LOCUS       DAOPJG010000001        81207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_278_length_81207_cov_7.770098, whole genome shotgun sequence.
ACCESSION   DAOPJG010000001 DAOPJG010000000
VERSION     DAOPJG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 81207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 81207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81207
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_81207_cov_7.770098"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1514
                     /locus_tag="PKU75_00005"
     CDS             144..1514
                     /locus_tag="PKU75_00005"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HPF79285.1"
                     /translation="MVAVDRSRLAVLLQQEADAYAAAHPRSRELYDASSNLFGHVPMT
                     WMNKWSGGFPLYLDHAQGARITDVDGHTYVDFALGDTGAMAGHSPDPTIKAVTARVGD
                     LGGLTTMLPTADAQWVGAELTARFTLPLWSFTLSATDANRWAIRLARLATGRSKILVF
                     GYCYHGSVDETFAVTNPIHGGTDSRPGNVAPPVPVDLTTRAAVWNDLESVEAALRHGD
                     VAAILTEPALTNIGIVLPEPGFLAGLRELATRYGALLMIDETHTFSAGWGGATTAWDL
                     EPDIFVIGKSIGGGIPCGAYGLSENVASQVTHHTDKGDADIIDVGGVGGTLAGNALSL
                     AAMRATLGEVLTPQAFEGMIDLATAFTQGVEAGIAEFDLPWSIAQLGARAEYRFAYPA
                     PRTGEEAAAAADDELDAYMHLAMCNRGILMTPFHNMALMCPATERADVDRHTDAFREV
                     VGALRG"
     gene            1520..1798
                     /locus_tag="PKU75_00010"
     CDS             1520..1798
                     /locus_tag="PKU75_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79286.1"
                     /translation="MTAPGLAEPFLAGAEVAAALLAREEVRVGWDAPSALPRMTIGML
                     ACHLGRQFIRSGQLLSAPATDGADLLGSAREHYRRPSRSRPHRSTIRP"
     gene            1795..2166
                     /locus_tag="PKU75_00015"
     CDS             1795..2166
                     /locus_tag="PKU75_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79287.1"
                     /translation="MTRDLDTEDASAGFDAMLERCLPALAEVTSLLRSGTAHDPVTIP
                     WQGWSRRQDDYLLTRLVEIVVHSDDLAHSIGAPTPEFPSAAYDPVLHLLADLAAERHG
                     QSALVSALTRRERMPGTISAF"
     gene            complement(2196..3137)
                     /locus_tag="PKU75_00020"
     CDS             complement(2196..3137)
                     /locus_tag="PKU75_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPF79288.1"
                     /translation="MTGIHDAITLRFLAAPTDAGQSGSVSAGRVLEWIDKAGYAAVAR
                     WSGTYAVTAYVGNVRFTRPVEVGHLVEVTARVVRTGRTSMDVMVEVFSGPPSTGELDP
                     STHCLMVFVSVDGDGKPRPVPALEPKGEAERVLRDWALRRAAVRHEVEEAMKRVVFTD
                     AGTAPRITMRFLAAPTDVNWGGKVHGGIVMRWIDEAAHVLATSWTGDPRNVAVFTGGV
                     RFYRPLRIGHLVEVEARLLHTGRTSMHIGVHVRSGDPATGEMEVTTYCRTVFVTIDEQ
                     RKALPVTPWTPVTDEDRRLDEHAVELVAIRARHEPGS"
     gene            complement(3134..3889)
                     /locus_tag="PKU75_00025"
     CDS             complement(3134..3889)
                     /locus_tag="PKU75_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPF79289.1"
                     /translation="MLLSGVPEGADALRRMKLAIAGLIAVTVFGTVGYMILAGQSATA
                     HLLDTGNQVALVDRDDTRFAGTDLPHVLGDASVNEVLQEAGIMQARALICALDTDAET
                     TYATLSARSMRPDLVIISRARTLDSKEKLVLAGATRAVNPQLIGGRRMASFALHADVA
                     EFLDEVLHDDDFGHRIQQVRLAEGSPFAGLTVADLDVPARFGCQQLAVRAPRKGAFVA
                     APPDSTSLVAGSTLIVFGTPDQVERVRAWAVPR"
     gene            3974..4408
                     /locus_tag="PKU75_00030"
     CDS             3974..4408
                     /locus_tag="PKU75_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79290.1"
                     /translation="MTTLSETLLTPERRPQTVDALVGVVEDEVASKSGLSGTAIKTAY
                     KAARKIDDRIVRRAIAGMLPEFLDQLEPFWAGRGEQPFGAHLAGQGERASEALLAVTD
                     ARAANPKHAAVAKVYGMLRGKAKDHVPAALPRVGTALERQMS"
     gene            4418..4984
                     /locus_tag="PKU75_00035"
     CDS             4418..4984
                     /locus_tag="PKU75_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HPF79291.1"
                     /translation="MDDVMEAFRAVPRAEFLRAGDRRRAAYDGPIAIGHGQTNSQPRT
                     VADMLRLLDVRAGHRVLDVGAGSGWTTALLAHLVGPQGLVIGVERVAEIAEFGATNLA
                     AAGMPWARLRLAEPDVLGAPDEGPYDRILVSAQPKVLPQSLIDQLRMGGVMVIPVGAR
                     MLRVVRTTDGHNISTHGYYRFVPLVTND"
     gene            complement(5009..5452)
                     /locus_tag="PKU75_00040"
     CDS             complement(5009..5452)
                     /locus_tag="PKU75_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79292.1"
                     /translation="MNSPQIAPITDADLPDVARLREHWTSGEPAGPDADFVAGIRQWW
                     ASENRVGWLARVDGYAVGMANAAVFTRMPRPGRPPARWLYVANVWVDPYHRRQGIGRA
                     LMAAAIDWARSEEMVRIVLAPSEMSIPLYASLGLRPATDLMRLDL"
     gene            complement(5449..7404)
                     /locus_tag="PKU75_00045"
     CDS             complement(5449..7404)
                     /locus_tag="PKU75_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPF79293.1"
                     /translation="MPGQSRFAALEPLLERVSKPIQYVGGELNSTVKDWDCGGHGPGG
                     EDLTVRWALMYPDAYEVGVPNQGVMILYEVLNERPDALAERTYAVWPDMEALMRAEGV
                     PQVTVDGHRAVREFDVFGISFSTELGYTNFLNALDLAGITLHAAERGEDEPIVIVGGH
                     AAFNPEPIADFIDAAIVGDGEQAVLQVTDVVTDWKAEGRSGGREELLRRLAHTHNVYV
                     PSFYDVTYLPDGRIRRVAPTEPGVPWRIAKNTVMDLDEWPYPKQPLVPLAEAVHERMS
                     VEIFRGCTRGCRFCQAGMITRPVRERSITGVGDMVERGLAATGFEEVGLLSLSSADHS
                     EIAGITKGLADRYQGEQVGLSLPSTRVDAFNIDLANELTRNGRRSGLTFAPEGGSERL
                     RKVINKMVTEQDLIDTVAAAYGAGWRQVKLYFMCGLPTETDEDVLQIADLAKKVIDTG
                     RKVSGRKDIRCTVSIGGFVPKPHTPFQWAAQLGADETDARLAKLREAIRAERKYASAI
                     GFRYHDGKPGIIEGLLSRGDRRVGRVIERVWREGGRFDGWREHFSYDNWIAACEAELP
                     AYGVDVAWYTTRERGQTEVLPWDHIDSGLDKDWLWADWQDALDETEQDDCRWTPCFDC
                     GVCPQLGTHIQIGPTGRELLPLSVTRS"
     gene            complement(7353..7826)
                     /locus_tag="PKU75_00050"
     CDS             complement(7353..7826)
                     /locus_tag="PKU75_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79294.1"
                     /translation="MRDRSSVVYEPFPGFVGWADGFDSTTVDAYANRLDEARAVATPE
                     AMAAAVQVATRYAAVDTGAIEGLYSTNRGFTRTIAEQTPTWQEALRQHGPHVERSINV
                     ALNGYEMVLDLATGRRSPSAAVWAMSRSAWKSGSPILVGCPDSPASPPSSRCSSA"
     gene            complement(7915..10173)
                     /locus_tag="PKU75_00055"
     CDS             complement(7915..10173)
                     /locus_tag="PKU75_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPF79295.1"
                     /translation="MTRFDDGTDAAVVLTQLRAGVTALQGLCARPSTAADRAEWLALV
                     EQSQAVVNAVTAAQDQAIVRTVCIDATMDEDGADVEYHRALGHISVDGPDLVGTRLCV
                     ARSFVTGRVDTAVRLAADRRPQYADDTPGPFEPDPEVGPGQPSGLSGLAAAMAAGRLD
                     GYRAARVAAELVDAPAEVAEQVIAELESRMQSGTAMQLARAARTVLNRIAPDLVRARA
                     ERARKARSLRRWPGEPGVDTWMGSFPAEQAMTAWAAVDALAHDYVAAGHPGGIEAARA
                     DALIDLVTGNATINVTLGLTVPADALHDDLSNDNPADDDPGDGLGSENPAAGDNFGDG
                     GEGDDDNNPGDGHSEHPAVGDNVGDGGKPDDDDSAGDDNSGDDDNSGDDDNYGDDDNP
                     GNGGEDGGDGGRPPADSPVPSCDTDDDEESPIPDSGGDSHEMPAADENPRPDDSTGEP
                     AATATAGERSGGLAFWRQPRPETRDASCAGIPDALVEVMGPGRGEPVLASRAWLTRNP
                     AVRFRTEPAACAPATGSLSDPDRRLDSAAYRPSAALTRLVKDRDGRCRFPGCTISVRY
                     CDLDHVRPWPTGQTSEVNLLALCRRHHRIKQAPGWRVRLGPGAVATWTDPTGRDHITY
                     PLDRLTPLILPAVPDSPAAGDPDPGEASSGAKAGAPAPGAAGPVEPAQPGPLADPYRD
                     RFSVLEYVFEHDLNTHVKTRPKSRRRRLRNRHCWYAVQHGPITYYHPCDEDTPRVIVR
                     TTALPTFDAPPF"
     gene            10606..10779
                     /locus_tag="PKU75_00060"
     CDS             10606..10779
                     /locus_tag="PKU75_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79296.1"
                     /translation="MARKVLLNTARSQRRRGAVAVRIASQPDAGVAGALDDLAVARAD
                     LTRAWAQLSADEQ"
     gene            10861..11091
                     /locus_tag="PKU75_00065"
     CDS             10861..11091
                     /locus_tag="PKU75_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79297.1"
                     /translation="MRELSLELASGRRIRVVVVGDAFYAWVADIAPADARDDAIAHIT
                     LVDGSARTPVLTEWAGSRGRAACPTTPGSSRQ"
     gene            11121..11615
                     /locus_tag="PKU75_00070"
     CDS             11121..11615
                     /locus_tag="PKU75_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79298.1"
                     /translation="MVCTTKFAESPEQPAIPKDAPALAVEKRDGLFMVLVGDESSAGL
                     CLFDKATLADPTKPAAWDPVQLTSGVTPPVQIGPRDIGDVSGAGYGRWGDPGEEMTWS
                     VAAVEGYAGAEVRGIAAVTPRGRLTALMIGRWFTIWWPSITHAGRLTYELLLADGSVV
                     TVTR"
     gene            complement(11722..13566)
                     /locus_tag="PKU75_00075"
     CDS             complement(11722..13566)
                     /locus_tag="PKU75_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPF79299.1"
                     /translation="MPTQVNHPTEEDIDSSLLENQAQSVGHLFRERVAKTPDAIGFWY
                     AEGDDYVKVPWREVKDHAYAMAAGLIALGVGEEERVAIASGTRYEWALADLANMCAGA
                     ATTTIYPTTIADDVAFIISDSDSKVVFAENEDQLEKLRSIRDVIPSVSKVVVFDGDGD
                     GDWVMSLEDLEILGRQELERNPGVVDGRIDNLTPDRLSTLIYTSGTTGRPKGVRLPHS
                     NWTYIGASVDAIRILGPDDLQYLWLPLSHVFGKMLLTIPLQIGFPTAIDGRIDKIVDN
                     LAVVKPTFMGAAPRIFEKAHGKIAMMMEEEGGLKLKLFNWAGSVGAQVSELREQGKSP
                     SALLGAQHKIADKLVMSKIRERFGGNIRFFISGSAALNKDVGRWFDGMGLKIAEGYGM
                     TESSAASTVNRMVAWQYGTVGWALPGMEFKIAEDGEVLMRGPGVMQGYHNNPDATAEA
                     LDPDGWLHTGDIGEIDERGFIRLTDRKKDLFKTSNGKYVAPSLIESQFKGMCPYISQI
                     VVHGNDRNFVSALVTLDPDAITGWAAQHGLEGKSYEEIASSDQAREMIQGYIDELNGT
                     LNRWEQIKKFTILGSDFSVESGELTPSLKVKRKVVGERYKAELDAHYN"
     gene            13690..14334
                     /locus_tag="PKU75_00080"
     CDS             13690..14334
                     /locus_tag="PKU75_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79300.1"
                     /translation="MAGGAFSSTRRRVSRTVPAGARRGRVLLPSLILVATLASAPFGG
                     LRATEAQPPAALKAGEVIATGPFDVTLGKVVTLDELSVNDLALPPASPGGRLMAIELT
                     VTNTTDEPLSPLRITDAIGGPRAAVEPWPGQDEPHGELVAVADGTRVRTVNPGLSVQA
                     VLVYPQPASWTADRIDLDVYDFRFQEEDPLSLDPDTWLLHSVVASGEFPIKESA"
     gene            14331..14924
                     /locus_tag="PKU75_00085"
     CDS             14331..14924
                     /locus_tag="PKU75_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79301.1"
                     /translation="MRRLTRIAAVVATLAAGHGITTLLPTDKVAAAPFVTAAAVGEHV
                     RLPYAEVRLAGIHLATKVQGQPTTATAYGRFLVLDLEVTALREPLLLAAPYLRDGEGR
                     RYAADTGAQCATAVTLPTGLPRYARMCIDVPRRALDGATVVIARGAIEIDGYRRDAVA
                     EIELSLDSTAAEQLWNTAAPVTGFLSGPTPPGDWARA"
     gene            14921..15544
                     /locus_tag="PKU75_00090"
     CDS             14921..15544
                     /locus_tag="PKU75_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79302.1"
                     /translation="MSGWWRANRAWLAALPVAAAVGAGASAYQVRTFWWESGMHRQVA
                     APAPGEPAGLTERYRDGQGETSRTLSVRLLGIDPAPPDPTPPDPAPPDPGPAGGAPGA
                     AAYRVRLEFSARPDQELRPCRIAVLGADGSRYEAEPGLSPCLPEDHPGPSASVIEGDP
                     RGHVEPGEERPATWQVAPVIQTAAAARIVAVRVWFGYPEYVELRAEP"
     gene            complement(15508..16794)
                     /locus_tag="PKU75_00095"
     CDS             complement(15508..16794)
                     /locus_tag="PKU75_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79303.1"
                     /translation="MEAAGRELGRIGRDTGRLLLRHWPALLTLGLLGAAWRAGAVWLA
                     VIVSDHSGIAAQWLLALAPVGYLLAVVGMLRVCRGTLPRLRAARDATAPRATTEGRPT
                     RLLDIAASVLIPFLGAYIAYGLFDQDLARFTNVAAFDEFNQFDLTREVDYDFMGRLGL
                     YAWPIALAIVVGAWVARWALGIAEARTGWVALGLLGALAEVYYTGQLAVQSAMLRTGG
                     LDWLATRRASSSPAQLWDWVTAHASWLADGLAVVGGLAGALLGSAEAVLLVPIAWLVV
                     ATIVLGHNVFPEHEAAEGQRARWLRWPVVRSFADDILERFGPFADAVRVLGAIGLIPM
                     LGLCLIMLLIVRIPTLVSYAVRAVVGPVKTDTWLAFSPIEGAAGLALSLTVLAPLLAA
                     TVAWVLDRAATTAAVQGGEVQGAEVQGSARNSTYSG"
     gene            complement(16842..17798)
                     /locus_tag="PKU75_00100"
     CDS             complement(16842..17798)
                     /locus_tag="PKU75_00100"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPF79304.1"
                     /translation="MLRWTDPAATPDALRPCVATFGNFDGVHRGHRVVIGTLLAEAKA
                     RGLPAVAVTFDPHPIQVLHPERAPELISPGSLHEQLLEETGIDGLVVVPFTVDYAQQS
                     PEEFILSTFVTGLGVKALIVGADTKGFGRGYTGDVDLIRELGQRHGFDVVVVPDQGNA
                     ARWSSSAARERLREGDVAGAAEILGRPHRVVGVVVHGFHRGRELGYPTANLERRSRGV
                     VPADGVYAGWFTRLDLPPDHPDHRLPAAISVGTNPTFDGTERTVEAYCLDRTDLDLYG
                     ETVAVDFVEHLRPTLRFDSIEELLDAMAGDVVAAHAVLDHRV"
     gene            complement(17809..18744)
                     /gene="truB"
                     /locus_tag="PKU75_00105"
     CDS             complement(17809..18744)
                     /gene="truB"
                     /locus_tag="PKU75_00105"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPF79305.1"
                     /translation="MGTDTRAPRREPAPDGLLIVDKPGGWTSHDVVAKVRYLAATRKV
                     GHAGTLDPMATGVLVLGIGRATKLLTFLVGCDKSYAATIRLGASTVTDDAEGEITEAT
                     PVDLQPGALEAAVAGLTGEIQQVPSAVSAIKIDGVRSYARVRGGETVALAARPITVSR
                     FEIRDRRSVEADGIPMLDVDVEVDVSSGTYVRALARDLGAALGVGGHLTALRRTRVGA
                     FTIDVAHPLSDLETHGPGELPIQPLDATAQAFTRRTLTADEARAIGYGQRIPSAQPGR
                     REPVAAVGPDGRFVAMLDESGPRAKPHVVFAPAAS"
     gene            complement(18751..19224)
                     /gene="rbfA"
                     /locus_tag="PKU75_00110"
     CDS             complement(18751..19224)
                     /gene="rbfA"
                     /locus_tag="PKU75_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPF79306.1"
                     /translation="MVDTARARKVAERIKDVIAGNLDATVKDPSLGFVTITDVRVTGD
                     LQHASIFYTVFGDENQAEQTAKLLQQNTGKLRSLVGKHLGIRLTPSLEFLADALPDSA
                     AHIDGLLREAKARDAEVAATAAGAAYAGESDPYKHGDEDDDDAADAAADAEISRS"
     gene            19693..20364
                     /locus_tag="PKU75_00115"
     CDS             19693..20364
                     /locus_tag="PKU75_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPF79307.1"
                     /translation="MSTITIAPRQVAIETKDGLVTRTLTSGKHRRIRGATYTIVDLRE
                     QLLTVAPQEVLTADGAQVRVSAAVRWAVGDPIAFHEKAVEPLSSVYLATQVALRDALG
                     AVGMDALAQRGARLPVAEITAAVAAVGLEVGIRVVEVIVKDVILPAELRAAATAVLAA
                     KQRGLAQLEAARAETAALRSLANGAQLLDRHPALATLRAIQSAPPGTQLVLRLGGGEE
                     RAPGQ"
     gene            20516..21154
                     /locus_tag="PKU75_00120"
     CDS             20516..21154
                     /locus_tag="PKU75_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79308.1"
                     /translation="MAGGVVGRSVRAAALTALALGVSGLAHSAGGRPLSLPGVLVALC
                     ALGPVTWAVSASRLRLGRLVVVLGAGQALAHISFLIAHGTSAASALSHAAAPMSGLPG
                     PALSGTALSGTASSGTAHGGHVAALSGPALLPSGRMLLAHGVATVVLALLLAGGEAVL
                     WRVLGALTRLPAAVSVPPFARLSGIPDRLHIAPPALWADARAMRGPPGRVTA"
     gene            21322..21972
                     /locus_tag="PKU75_00125"
     CDS             21322..21972
                     /locus_tag="PKU75_00125"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HPF79309.1"
                     /translation="MISRNRSRLAAASAALLLSTSLAACGSGDSSSSSSPGTSAASST
                     AAGSAATSASDSGTPASTESASSGSTGRIGPVTITDGWAKATDMEMSAVFGTLVNNSD
                     KAIHITGGSSDVAGSVETHEFVKDAGGNMVMQKAPNGFTIEAGKTFVLAPGANHIMLL
                     GLKDKLVAGDSLTVTLETDLGQVPLSVMIRTFDGANETYNPSHSMSGSASMSMSSS"
     gene            21969..23219
                     /locus_tag="PKU75_00130"
     CDS             21969..23219
                     /locus_tag="PKU75_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HPF79310.1"
                     /translation="MTPAAGCPAGRTTPAGPGRRGVLTTAVVAGLGLGGCSLTAGSPS
                     GTAESADLVASGATTAGAAPAEAVQPFRAARQPGIRGRVQPFAAFVAFDLTPGSSAPD
                     VRRILRIWSDDIERLMSGRPPLTDPEPELARHTAGLTVTVGVGPRIVELVRGADAVPA
                     WLGPLPAYPQIDKLEDRWSGGDLLLQICADSPTTLAHAQRRLTLGVRSMAETRWVQRG
                     FREPLDTGEHLIGMRNLFGQVDGTIQPADDEPVFIGAGGPDWLRDGSSIVIRRIRMDL
                     DKWEQVDRLGRENAMGRRLTDGAPLTGGAEIDIPDVDAKDALGFPVIDPGAHVRRARA
                     TTNTERFLRRPYSYDDGTGEQVDAGLIFVAYCADPLAQFDPVQKRLAAKDLMNIWTTP
                     IGSAVFAILPGPGDGESLGQALLA"
     gene            complement(23230..23376)
                     /locus_tag="PKU75_00135"
     CDS             complement(23230..23376)
                     /locus_tag="PKU75_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79311.1"
                     /translation="MIDTVRRLPVSPVPVYAVHRRHPSAPERIVLELLEILAGALLPE
                     PTSI"
     gene            complement(23794..26661)
                     /gene="infB"
                     /locus_tag="PKU75_00140"
     CDS             complement(23794..26661)
                     /gene="infB"
                     /locus_tag="PKU75_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPF79312.1"
                     /translation="MSKIRVSALAKEVGVPSKVLLERLNEMGEYVKAASSTIEAPVVR
                     RYHEKYPTEAAAPAPAPAASAPAGPKPGGPKPGPARPPRAAQPETDAAPTPAPAPAAP
                     AAAPAQAAPAAAKAPEAPADAPAPAAPPASAPSTPPAAEPGPAAGPAATPGPRPGPAK
                     PAPAAPAAREGGAAGPRPAAPRPGNNPFSSSQGMRQGREGGGGAGGHAPRPGNNPFAA
                     SQGMPRPQARPQRPGGAAAGPGGPRPGGPRPSMMPDRSAIARPGERPARGGAGRPGAG
                     AGRPGGGGFAGRGPGGGTGFGGGGGRPGGGGPGGRGGTQGAFGRGGGRAKQRKSKRAK
                     RQEFEQMSAPTIGGVQIPRGDGRIVQIRQGASLTDLAERIDANPASLVTVLFHLGEMA
                     TATQSLDEDTFALLGAELGYEIRMVSPEEEERELFESFDIDLEIGIDSEDEEDLQPRP
                     PVVTVMGHVDHGKTKLLDAIRNANVVAAEHGGITQHIGAYQIHKEHEGVDRAITFIDT
                     PGHEAFTAMRARGAKVTDIAILVVAADDGVMPQTIEALNHAQAADVPIVVAVNKMDVD
                     GANPAKVRQQLTEYNLIAEEYGGDTMFVDVSAKQMLHIDDLLEAVLLTADAALDLRAN
                     PDKDARGVAIEANLDRGRGATATVLIQSGTLHVGDAIVTGSSHGRVRAMLDEHGNTLD
                     EATPSRPVQVLGLSSVPRAGDTFVVAPDDRTARQIAERREAADRAASLAKARKRISLE
                     DLNAALAAGKVDTLNLILKGDVSGSVEALEDALLQIDVGDEVDLRIIDRGVGAITMNN
                     INLAMASNAIIIGFNVRAEGQNAEYAEREGVEIRYYSVIYQAIEEVEAALKGMLKPEF
                     EEVELGTAEIREIFRSSKFGNIAGSIVRSGEIKRGAKARITRQGVVIAENVEVAGLRR
                     FKDDVTEVREGYECGINLGSFNDLQLGDLIATYEMREKPRA"
     gene            complement(26721..27110)
                     /locus_tag="PKU75_00145"
     CDS             complement(26721..27110)
                     /locus_tag="PKU75_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HPF79313.1"
                     /translation="MGKTDRTGLRPSTSTAPAGPIRTCIGCRVTDSRSALLRIVAGEG
                     HDGHPAAVPDPAARTPGRGCWLHPDPECLALAVRRRAFPRALRMSAPLEVTLVAAYLE
                     ACAPEANLTEPDRKRVNRDEPPMSTQK"
     gene            complement(27112..28263)
                     /gene="nusA"
                     /locus_tag="PKU75_00150"
     CDS             complement(27112..28263)
                     /gene="nusA"
                     /locus_tag="PKU75_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPF79314.1"
                     /translation="MDIDPATLRALEAERGISMDVLVPALEKALLSAYERTDGHYRHA
                     RAELDRKSGHVTIWAQEEIELPEEELDAEAELGEEARPRRPRYELGPEFDHTPQDFGR
                     VAAATARHVILSRLREVEDDQILGEFKGREGDIVAGVIQQSPNPRHVTVSFGSVEGIL
                     PSAEQVPGESYKHGDRIRCYVVTAKRGLRGPQIELSRTHPNLVRKLFALEVPEVANGT
                     VEIVQLAREAGHRTKIAVHSKVPGLNAKGACIGPMGARVRAVMAELGGEKIDIVDWSE
                     DPREFVAAALSPARVQSVEIVDPKTQSARVVVPDYQLSLAIGREGQNARLAAKLTGWR
                     IDIRPDVASPAAGGGTHPHHGSGGQGEGQAPGRTPGQTQGEAPGAAPTR"
     gene            complement(28264..28773)
                     /locus_tag="PKU75_00155"
     CDS             complement(28264..28773)
                     /locus_tag="PKU75_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HPF79315.1"
                     /translation="MSAADRVREIAAAAVADPALVIDGVEVSAAGKRKLVRITLDRVP
                     AAGPDGWSDAPTPALSLDEIADHSRAIGDALDRDEPFGGAAYVLEISSPGVGKALREP
                     RHYRRNVGRLLKATTADGEVTGRIVRASEESVVLAGGEPPAETDLGYAAIERASIAVE
                     FTSTEEKDE"
     gene            28865..>29307
                     /locus_tag="PKU75_00160"
     CDS             28865..>29307
                     /locus_tag="PKU75_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79316.1"
                     /translation="MSSPRPSADAGRPRRAALRGGLLAGAGLALSACGIRLEDDAPKV
                     PLVPTRTPIPGEAELLALLHAVSALAASAPAAYAAGLGRQAAVLDSALRERGVPQSSL
                     SPSPSASGSGATSSVSTTVSSTTAVSGATSSASSTVSGATSSASST"
     assembly_gap    29308..29407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29408..29971
                     /locus_tag="PKU75_00165"
     CDS             <29408..29971
                     /locus_tag="PKU75_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4439 domain-containing protein"
                     /protein_id="HPF79317.1"
                     /translation="TSSASSTRAVVGLLLAALPVADAALRPVLGSMTASHVRLSTLLD
                     DAVPAPARGSVPKAAGLALLAATREAVYGTEVAAARFADAQRTKAIAALERLRGWATT
                     LEEDLGADAPPMPTAYALPLDATDNATAKRLIAHLTRSLVQSYAAVLPTVSGDAEATL
                     AATGWLSSAVTLDGSWGATWDPFPGLS"
     gene            30017..30718
                     /gene="msrA"
                     /locus_tag="PKU75_00170"
     CDS             30017..30718
                     /gene="msrA"
                     /locus_tag="PKU75_00170"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPF79318.1"
                     /translation="MIFGRQPKPMPTADTALPGRAQPVFAVPTTHEVLGTPLAGPWPD
                     GTEVIHVAMGCFWGAERIMWRLPGVVATAVGYQGGFTPNPTYEEVCTGETGHAEAVLV
                     AYDPARITPSRILKAFWENHDPTTPNRQGNDVGTQYRSAIYWTSQEQQAAVRATHAAF
                     QGELTKAGASDITTQELPAEQAGTFWYAEAYHQQYLHKNPGGYCNHGPNGLTCPVGIA
                     DLPAQVDVEPPAEHR"
     gene            30715..30903
                     /locus_tag="PKU75_00175"
     CDS             30715..30903
                     /locus_tag="PKU75_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79319.1"
                     /translation="MTENDIRGVAGLCGTCAHALIRPTNKGTVYLRCALAATDERFAR
                     YPRLPVHECPGFTRPGSA"
     gene            complement(30900..31100)
                     /locus_tag="PKU75_00180"
     CDS             complement(30900..31100)
                     /locus_tag="PKU75_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79320.1"
                     /translation="MSKDQRRGIVGLVAVLYGMGVAFGPSLVDNDALWKAWLGVGAFV
                     VGALWLALMVFPWAQGRRTETP"
     gene            complement(31097..31285)
                     /locus_tag="PKU75_00185"
     CDS             complement(31097..31285)
                     /locus_tag="PKU75_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79321.1"
                     /translation="MGIETGLAVTGVIAALFGVGFAFLPKFVDSPRVERGYTMAGSLL
                     VLACALALVVVPWMGDRA"
     gene            complement(31547..32356)
                     /locus_tag="PKU75_00190"
     CDS             complement(31547..32356)
                     /locus_tag="PKU75_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPF79322.1"
                     /translation="MTTTTLPLPTRPGPRAARAAQPAQPIQRRAFVEFIMGMPISLHV
                     KAIDTSLTDIDQAAGNVFATLRKVDEVFSLWRADSELRRLQAGAVLAGECHPWQAEVL
                     ELCLGAEERTGGLFTAWRAAPGEVPAYDPTGVVKGWAIEQAAALLRLVPGISFCLNAG
                     GDMVVGFGTGMRGVAPSWSIGIEDPHQAGRIAATVEVVEGAVATSGTAARGSHIADPR
                     TGEVVSRSGSTTVVGPDLVWADVWATAAFVDPAAVRDLMTERAPESSLTVL"
     gene            complement(32353..32898)
                     /locus_tag="PKU75_00195"
     CDS             complement(32353..32898)
                     /locus_tag="PKU75_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPF79323.1"
                     /translation="MSTLSALVLLFSYPTSRNAAASSSVAAAAPTTTESSSGTTDSTD
                     SATSQSPTGSATTDDTSSDATTESSTPSSSSTSSSSSDSSSSDAGTQTYAGDAVNTQW
                     GLVQVQITVENGKITKSEVLQVPWSNHKDQEINSYAVPILNQETVDAQNSSIDMVSGA
                     TVTSVGYIQSLQSAIDQAHLS"
     gene            complement(32937..34394)
                     /locus_tag="PKU75_00200"
     CDS             complement(32937..34394)
                     /locus_tag="PKU75_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HPF79324.1"
                     /translation="MTATAERTTTLPTDPPVRWVGSTPPTERPAAAAPLRRTRTWWRD
                     TSGAAVWLLLLGVTALWIHGGGLADVGSVGGFLSSFGRLTGLIASALLLVQVFLMARV
                     PWFEKAWGQDELVRSHRLIGFTSFNLMLAHILLITLGYAAGTSEGIWGTIVDFTVNYP
                     GMLLAIAGTACLVMVVVTSIKKARARLRYESWHLIHLYGYLGAGLALPHQLWTGQEFL
                     DSRAATVFWWTLYAVVAAAVLAFRVAMPLFRSRRSDLRVVAVTAEAPDVTSVTLRGRG
                     LNRLGAQGGQFFNWRFQDGPGWTRANPYSLSAAPTDDTLRITVKHLGAGSARLADLQV
                     GTKALIEGPYGRLHPGVRGNRKVLLLGAGIGITPMRALLESLPQSAGDVTIVHRASSR
                     DELVLGEEIEALAAHRGARYVVVDGHRIPGRDSWLPESAAHLGDAEALLQIAPDIADH
                     DVYICGSGPWMAAAEAAVKDAGAARKAIHIEHFEY"
     gene            complement(34518..35285)
                     /locus_tag="PKU75_00205"
     CDS             complement(34518..35285)
                     /locus_tag="PKU75_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HPF79325.1"
                     /translation="MNDLPPEVWLGLAALVAGWIDAVVGGGGLVQIPALLLGVPGATP
                     AQVLATNKLSSIAGTVSSTATYCRRVRPDLRTAVPMAAVALVGAMGGALIGVHIPKSA
                     FNPIVLVMLVLVGAYTLLKPDLGSVTALRHIGTRHTALAMLTGFAIGVYDGALGPGTG
                     SFLVFTLVGLLGYAFLPATAKAKIVNLATNIGALVVFIPGGHVMWRTGLIMAACNVIG
                     GYAGARTAVARGSGFVRAVFILLVGAFIIRLGWQIAG"
     gene            complement(35282..36049)
                     /locus_tag="PKU75_00210"
     CDS             complement(35282..36049)
                     /locus_tag="PKU75_00210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPF79326.1"
                     /translation="MSHERSQPGVEAVVFDWGGTLTPWHAVDIGEQWRVFAREVHGIP
                     VDSPEVSEADLRAAHDLADRIIAVEDDAWARGRADHTSAHLDAILEAAGVDPEHDRHL
                     LALAAYRRFWEPHTITDPQVRPLWEGLRENGIKIGVLSNTIWTRDYHRELFARDGVLD
                     LIDADAYSSELHVVKPHAEAFAAILRQLDVEPGAAVYVGDRIYEDVWGPQQIGMRAIW
                     IPHSDLPPRQRVAVDATPDATVHELREILGVVTGWTA"
     gene            36095..37591
                     /locus_tag="PKU75_00215"
     CDS             36095..37591
                     /locus_tag="PKU75_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HPF79327.1"
                     /translation="MTAPAPSGPVPVNPWPALWALVVGFFMILVDSTIVAVGTPALMA
                     AFDTDVTAVLWVTSAYLLAYAVPLLITGRLGDRLGPKRVYLAGLTLFTLASLWCGLSG
                     ELSDSIGMLVVARVFQGLGASLMTPQTMAVITRTFPPERRGSAMALWGAAAGVATLVG
                     PILGGVLLDSLGWEWMFFINVPVGIIGWLLAWRLVPRLETHSHSFDWVGVALSAVGLF
                     CGVFAIQEGHAYDWGQITGVISVPLLLVVGAVLLGLFVWWQSRIRREPLVPLGLFRDR
                     NFSVSNVAISTVGFAITAMMFPFMIYAQAVRGLSPTRAALLLAPQAVMSIVLAPIAGK
                     LTDRMHPRLLAGFGLLTLAAVTFALSRVMTPGSSTWSILLVALVLGVANSFVWGPLAT
                     SANRNLPPHLAGAGAGVYNTTRQIGAVLGSAAIAVLMEARIAALLPGMPGGASSMAES
                     GAVAMPEAVKEPFAQALAESMLLPAAVVLLGFAAALFFERPRHQQTSG"
     gene            complement(37604..38722)
                     /locus_tag="PKU75_00220"
     CDS             complement(37604..38722)
                     /locus_tag="PKU75_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79328.1"
                     /translation="MPGIGPGETGVDTMRRSLVAGLMMVTSAALAGPAAGASDPVPPR
                     TPSPRTAAPDTTTPAAAVLTPTVLPGLGGSSASAAAVNDSGVVAGTALATDGRYYAVQ
                     WTGGRITRLPALGGAECQARDIDNSGRIVGGCMTAAGTFKPVMWEGGRLTRIPAPGDF
                     GMAVATSKAGIVGTYNVPGDDPFQTRRAFLWRPGQASARTITAPIGASAEGINDSGRV
                     VGTMRWLWADPSPLGQYRAWTWSAGTQTQLGTLGGGTTPNDINNAGQVVGTSIVTGSR
                     LVPFIWSSGRLRALPTGGRNSLTAQALNDGGVIVGTDGSQERAIRWASPSSPVTYLPQ
                     PSGTMLSQGQDVNRAGTVVGMAGSYDGSAFRFVAVVWK"
     gene            complement(38803..39435)
                     /locus_tag="PKU75_00225"
     CDS             complement(38803..39435)
                     /locus_tag="PKU75_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF79329.1"
                     /translation="MSDTAGWWEKHAFPSAVDLVLSDRLVGEWRRGLTAGAHGIVLDL
                     GFGSGPNLPHYPVAVEQVYAVDPSDEGWQRAGAAIADFGRPVTRIGTDAAAISLPDNS
                     VDVVVAAWSLCSIPDVEAALTHARRVLRPGGTLRLVEHGLSPRPWLAAAQRAMQPAWG
                     RLASGCHVDRDIERLVAAAGFDTSGLTAQTAFGVPTPWTWFVAGSATTSP"
     gene            complement(39442..41208)
                     /locus_tag="PKU75_00230"
     CDS             complement(39442..41208)
                     /locus_tag="PKU75_00230"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPF79330.1"
                     /translation="MAMRMSSLFLRTLRENPADAEVPSHQLLVRAGYIRRVSPGIYTW
                     LPLGLKVLKRVEQIVREEMDEIGSQELLFPALLPREPYEATGRWSEYGANLFRLKDRR
                     EGDYLLGPTHEEMFTLLVKDMFSSYKDLPLALYQIQTKYRDEARPRAGILRGREFVMK
                     DSYSFDIDEAGLDKSYDLHRSAYVKIFDRLGFHYVIVQAMAGAMGGSKSEEFLATAEN
                     GEDTYVRCDNCGYAANVEAVQVTAPPAQPYDALPASHVEDTPDTPTIETLVAHLNSAF
                     PKADREWTAADTLKNVVVMLVHPDGTREPLAIGVPGDREVDEKRLGAQVEPATVTAFE
                     EKDFAAYPTLAKGYIGPGALGEKGTSGLRYLLDPRVVEGTAWVTGADQPGRHVLDLVA
                     GRDFSADGTIEAAEVRPGDACPSCGSPLLSARGIEMGHIFQLGTKYAEALDLKVLDEF
                     GKLQTVIMGSYGIGVSRAVAAIAEGNHDEFGLVWPRHISPADVHIVAAGKDDSILTYG
                     EELTRALEAGGLEVLFDDRRQASPGVKFKDAELIGIPTIVVVGKGLADGTIEIKDRRS
                     GERRTVPVADAIAEITREVTSA"
     gene            complement(41265..42338)
                     /locus_tag="PKU75_00235"
     CDS             complement(41265..42338)
                     /locus_tag="PKU75_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF79331.1"
                     /translation="MPPAFTPTRAERAQKRAFEAMLAVPDRLRRRLDLHPHVVDGARL
                     DPDTHVGLAIMTKLGEPELDQLPVPEARRQLALDSWMFAGKPARVATAADLTIPGPAG
                     GIPARLYRPHPPAGSPPGLKPLGLLVYFHGGGWVLGDVDTHDPTCRLLCGLGHVAVLN
                     VGYRLAPEHRFPAAVDDAVAAFRWAHEHAEQLGLDPERIAVGGDSAGGNLAAVVAQVT
                     HREGGPMPAYQLLGVPVTQLGATTRSRELFGEGYFLTKANMDWYEANYLGPDGDPSDV
                     RASPLLAHDLAGLPPAYVAVAGFDPLRDEGIAYAEKMRAAGVDVTVRVHEDAVHPMLT
                     MPAAPLGQRVLAETAGALKTALG"
     gene            complement(42343..43320)
                     /locus_tag="PKU75_00240"
     CDS             complement(42343..43320)
                     /locus_tag="PKU75_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF79332.1"
                     /translation="MLGASTVVLLVLLAFVWPSYLSKVKDLPLVVAGPPAALTQIQVG
                     LGKSGAFDITTVADRAAAESAIKQRDAYGAVVASPSGVEFLTASAASTVVSQALNQAA
                     AGITAQAAAAGQQPPKVTVTDIAPLSPDDPRGAGFGVAGLPLAMGGMLGGVLISLLVA
                     GHRRRLAAVTGYAVLAGLLLAAVLGPWLGILVGSFVMEWAIISAALFATGAFIVGMNG
                     LIGRAGIAPGAIVTLFIGNPLSSLTIPKEMLPWHWGEIGQFFVPGAAGTLLRLESYFP
                     DASTLRPWLVLAAWALVGVVTLWFGRHADEEVLHIDGATEPETAPALAS"
     gene            43515..44108
                     /locus_tag="PKU75_00245"
     CDS             43515..44108
                     /locus_tag="PKU75_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPF79333.1"
                     /translation="MPKVTEARRLAVRDRIRDAALSEFMDKGLATTSMADIVSASGMS
                     AGAIYGHFTNKAELTAEVARGVIASRVDIVADSAVAEQIPTPSALLRTLFDTMPQDVL
                     DSGIVLQIWGAAGPSGELRPIAQQAFSTMRSVFTEYLAAWLVSQGHSRRAALARARKL
                     APAFIGLAQGYLVHRSVLGRDLADTYLDSVQLLLADT"
     gene            complement(44165..46372)
                     /locus_tag="PKU75_00250"
     CDS             complement(44165..46372)
                     /locus_tag="PKU75_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPF79334.1"
                     /translation="MTTPKKKPRWSAAQKAAAKKKDAGKGKRGADDAPRAKKPRWVDG
                     KPARPESSDKGKRRRDDSYAAQSDPRDRRPSRDDRRDDRGSRPYGRDERRDDRGSRPY
                     GRDERRDDRGSRPYGRDERGRNDRAPYRDDRGSRPYGRDERGRNDREDRGARAFRDDR
                     GNRGYRRDRDDRPFREERDRRPYGEDRDGSRGRDDRRPFREDGERGSYRDDRGDFRVR
                     DERRPSRDDRGSRDDRASRENRSSRDDRGGERFGVRPWERDERPTRRPAGRAGEAARH
                     TPRPVREERARSRGIPDTTPPPAPASVDHEDGGPTFADLGLPEAIVTALASRGIRRPF
                     PIQAATIPDALAAKDLLGRGQTGSGKTMAFGLPTIARLSGQRATPNRPRALVLVPTRE
                     LALQVSDALEPISRAVGVSQKVVAGGMPYPPQLTALDRGVDLIVATPGRLQDLIERGA
                     ALLDDVEIVILDEADHMADLGFLPEVTEIMDLIPEGGQRLLFSATLDRGIDELAQKYL
                     VDPVTHSTDDPTAAVSTMEHRVLLIEPSMKKTLTAQISGRPGKTLVFVRTQLGADRVA
                     RQLRESGIMAGPIHGGLNQSMRNKVLEAFKDGRLQVLVATDVAARGIHVDDVSQVVQV
                     DPPADHKTYVHRAGRTARAGSTGAVVTLALPHQRKAMEKIIEEVALSIAPERLDNAGA
                     AAEGVEPSERPVPDEVWQSIIAPRPMRGRRGPGRPRPDGAPRRARPAYGSRSR"
     gene            complement(46561..47406)
                     /locus_tag="PKU75_00255"
     CDS             complement(46561..47406)
                     /locus_tag="PKU75_00255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79335.1"
                     /translation="MLRTRTPTRVLTPSDLDAALGLCARNPADNVFVAARLLEAGMAG
                     PGQSVVGYEEDGQLQSLCFAGANVVPVETTAYTRGPIADRVRKWNRRAASFLGPAEQV
                     LGMWELLESGWPNVRAVRSNQPHLATRTPPSRLGVVGDERVRRARPGEVDLVLPAAEH
                     MFTHEIGYPPYRGSSRGYRSLLQRLLDQGHTYVIVENGRVIFKADLGSVALDCAQIQG
                     VWVSPELRGHGLGTSAMAAVVEHVLADVAPLATLYVNDFNTAARTTYERIGMTQIGTF
                     ATVLM"
     gene            complement(47462..48592)
                     /gene="ispG"
                     /locus_tag="PKU75_00260"
                     /gene_synonym="gcpE"
     CDS             complement(47462..48592)
                     /gene="ispG"
                     /locus_tag="PKU75_00260"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPF79336.1"
                     /translation="MPPPVLAARRPTRKIKVGKVDVGGDAPISVQSMTTTLTADVNAT
                     LQQIAELTAAGCDIVRVACPSQDDADALPAIARKSQIPVIADIHFQPKYVFAAIDAGC
                     AAVRVNPGNIRQFDDQVGEIAKRAKEAGVSLRIGVNAGSLDKRLLAKYGKATPEALVE
                     SAVWEASLFEEHDFHDFKISVKHNDPVVMVRAYEMLSERGDWPLHLGVTEAGPAFQGT
                     IKSATAFGALLSRGIGDTIRVSLSAPPVEEVKVGIQILQSLNLKPRKLEIVSCPSCGR
                     AQVDVYKLAEEVTAGLEGMTVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVKG
                     EVIKTVPESKIVETLIEEAMRLAEEMGEPVDDLSGAPTVTVG"
     gene            48742..49044
                     /locus_tag="PKU75_00265"
     CDS             48742..49044
                     /locus_tag="PKU75_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79337.1"
                     /translation="MTDAGLSSVIDLPDPGVLRALCDDETGESAVALRTHILHAQDTA
                     RSAEALIAQGIQLIGQERVDALERLRTERIRRSPMPHDQRGKRWGKLLTRARVAAH"
     gene            49056..50729
                     /locus_tag="PKU75_00270"
     CDS             49056..50729
                     /locus_tag="PKU75_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HPF79338.1"
                     /translation="MAQGMQAMRGDAIETAGDRAGRNAGLGRRIWESTGAGLVRRGLL
                     KGSARALTGVYGLYKVHPAVWRWTARNYHPQMEKLARLHAWMTCQFAYLDVPAYKEHL
                     RHNDFQFKWFDLTSYPPTDKDGYVKKFTEEARCWEGHLDRVGTVVDESSGSSGTPFNW
                     VRGKRELDTIHRNVAGFTTQVYPRRRLFVINAYSMGAWATGTNTGIAMAKIAMVKNTG
                     PDLDKIVDTITHFGPTYNYLVTAYPPFVKDLRDKLDSVGFDWDSYKLYGMVGGEGMTE
                     ALRDYCEERFIRVLSGYGASDLTIGIGGESRLTVWLRDQIVRDEAFKEALLGEGESRI
                     PMIFQYNPLETYLETNDKDELLCTLNSTTVLSPKLRYNIGDEAKLLTFPGLEALIAKR
                     PDLESGFREAQSIDRMKLPILLLYGRADSTVSFMGANIYPQDVEYGLYEGNPRANEIE
                     AFMLTLEETTDLDARPVVNIATREGVHLDDGSKAELGKACETGIVEHLMKVSRDFRQS
                     VEEDPSSTQIAVRVHDFETGPFEGGQSKIKKVYIRKDQPADPAQAEAAS"
     gene            50726..51187
                     /locus_tag="PKU75_00275"
     CDS             50726..51187
                     /locus_tag="PKU75_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HPF79339.1"
                     /translation="MRTTDFSKAPRRAQAEAMFIGSTRYNSLKEWAILSMRWRTFVKR
                     MKAMPGYCWHKVYWEPPYTLGTIAYFDTMEDLLTIARSPEHRQLMRWVADGTDDQATA
                     GFIRLYRAEPHGYTNGIWRAEDTSMAHIEEYTPLPGDTGPKPVDDPASEIR"
     gene            51199..52317
                     /locus_tag="PKU75_00280"
     CDS             51199..52317
                     /locus_tag="PKU75_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79340.1"
                     /translation="MTQIAIERVVTEAQFQEFADLPVRLHGHGRHVPLLQPVLHAWWT
                     GHFPQAEHGPVGFFLARREGAAVGRICLHHNHLFDDKIGAEAQLFGLCEFADAEVLAA
                     LLGTVEREARAAARTCLVGPVALLPNQTGGVITSGFGERGFMDSPWNPDYYPTAYESL
                     GCTRIFAGATWICENFDNLDPATTFRFDDARLAAEGLNVRRASRWRLGRDLESMRAVL
                     NTAFAVLPYYTQISASDLAAQTDGLEVLLDEALFLQLFKHDEPVAFVLAVPDISEFVM
                     ARGGRLGMRDQLALVATRRRYRTDAILIIKGTHPDAQGKGYLTLLSRELLRGLQAGDY
                     RRLRSTFVEDANAGSAAQYERMGGRPLHETTFYRKEIR"
     gene            52314..53330
                     /locus_tag="PKU75_00285"
     CDS             52314..53330
                     /locus_tag="PKU75_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79341.1"
                     /translation="MSWVEEVLAARAVWQRAPSAHNTQPWVLTPDADRLVLSWDPDRE
                     LVVADPTRRDLWLSLGALVEAIAITEAGAGRQLDVDWQIDAHHHRAAVLTDGGPGSAS
                     DDSFAGQQFSTDELLRRRTARGPYAATPVAESTVDDIAVAARLASTGLGLALVPAPLV
                     EVLLPASDRWSYSSPAQVAELRGWLRLDRNHPAHRRDGLSYEALAMTALQARALRTAL
                     SPKGWAILRRLGGPALLAAAGGIDGLGTVVALHTTRDAAADPVHVAESGRALLRVWLA
                     AGRHGLSAHPLSQLIDAPATAEKLHAALPGRDRFALNVFRIGVPDRVLPPSSRLTRFN
                     QATS"
     gene            complement(53390..53800)
                     /locus_tag="PKU75_00290"
                     /pseudo
     CDS             complement(53390..53800)
                     /locus_tag="PKU75_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
     gene            complement(53805..55133)
                     /locus_tag="PKU75_00295"
     CDS             complement(53805..55133)
                     /locus_tag="PKU75_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPF79342.1"
                     /translation="MLYLIGVLIVVLGVGASIALHEIGHLVPAKRFGVRVPQYMVGFG
                     PKVWSTKRGETEYGIKAIPLGGYIRMIGMFPPRPGEGERVSSTGRFTQLRDEARGQSM
                     EEIRPGDEDRVFYKLSTPKKITVMLGGPLMNLLIAAIVLTGLATLHGISVTSAKLSSV
                     SQCVDTTQAAQSSKTECTAEMPIAPANAAGLKPGDTVVSMNGVALQTWDDARALIRGS
                     ADKPLTMVVERDGQRRTVTATPMATTLPVYDKYGIPKTNTDGTYKTETAGFLGAAGTP
                     VLVKQPVSAVPGIIGEQISQTAGVILNIPQKMVGVAKAAFGEGERDINGPISVVGVGR
                     VAGEVASADDFGSTSDKVITLVALLGSLNLALFVFNLIPLLPLDGGHVAGALWEGLKR
                     RAFKLARKADPGPVDVTKALPLAYAVASLLIVMSVLLMYADIVNPVRLRG"
     gene            55364..56311
                     /locus_tag="PKU75_00300"
     CDS             55364..56311
                     /locus_tag="PKU75_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79343.1"
                     /translation="MPCPGPPLPHRPLPERLLAALATATPELVVAADEPGAGHWAGAP
                     YAVRDGARIVLAYRVRKPEGHGRGLANIIAVSDDGLTFREVARVESGTFGAASLERPA
                     LARLADGGWRLYVSCSTPGSKHWWVEAVDAPTLEELPVGTRAVVLPGDDTEAWKDVVV
                     DRAPDGQWRIWACRHPLDGGPDAADRMSTWFGTSTDGLAWLMHGPAMTPTEGAWDQRG
                     ARLTAVLPAGTEAGLSEASGIVALYDGRASAEENFRERTGVALGIPDRLVAAGGPVPA
                     ASGRALRYVSLVQTPAGLRTYFEVTTDDGSHALVTTLVP"
     gene            complement(56314..57504)
                     /gene="dxr"
                     /locus_tag="PKU75_00305"
     CDS             complement(56314..57504)
                     /gene="dxr"
                     /locus_tag="PKU75_00305"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPF79344.1"
                     /translation="MASVTTTRSVALLGSTGSIGTQALDVIARNRERFTVAALSAGGN
                     VGLLAEQAATFRPALVAAATGDPDAIRAAIAARVSAAGDRAAYRPEVITGADAATRVA
                     ASGADVVLNGITGSIGLRPTLAALESGSTLALANKESLIIGGPLVKAAAAPGQLVPVD
                     SEHSAIAQSLRGGRHAEVRRLVVTASGGPFRGRSRDELREVSPEQALAHPNYAMGRVI
                     TTNSATLVNKGLEVIEAHLLFDIPFERIDVVVHPQQWIHSMVEFHDGSTIAQMGPPRM
                     LVPIALGLSWPERLADIDEPCDWTRAQTWDFHPLDDEAFPAVRLARQVGTAGGTYPAV
                     YNAANEVCVDAFHDGTIGFLDIVDTVARVVEAHDGSPVESVEHVLAADAWARAHATAL
                     IQLD"
     gene            complement(57556..57915)
                     /locus_tag="PKU75_00310"
     CDS             complement(57556..57915)
                     /locus_tag="PKU75_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HPF79345.1"
                     /translation="MSVNAVRRLGPDASVVAVVREEYAAGRAVSVVPAGVDKAESLRL
                     FAEILDFPDYFGGNLDALYDCLRQVATDRPDDWALVWDAAGALRAGDPAAYDGIVGVL
                     ADVAADAGQLHVTVLDR"
     gene            complement(57918..58451)
                     /locus_tag="PKU75_00315"
     CDS             complement(57918..58451)
                     /locus_tag="PKU75_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HPF79346.1"
                     /translation="MKKADRQLLMLLGVAVCIALVWWAVGRGGEPGGATTSGVTTGGV
                     TTSAIATGGGEATTPKLPTAPTTGPGPKDPVTAPVTPVSGLDTIAESQLPAQGRDTLA
                     LIRAGGPYPYSEDDGVFQNRERILQQQPRGYYREYTVETPGSDDRGARRIIGGAKGDR
                     YYTDDHYNSFRQILEGE"
     gene            58504..59736
                     /locus_tag="PKU75_00320"
     CDS             58504..59736
                     /locus_tag="PKU75_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HPF79347.1"
                     /translation="MTSALTRLADGTVKQINVFTGTEVWTVPGRAHRPLVQEERAKHP
                     IRASQARSLCAFCETRRLETPPEKARLVRAGGGWEVLRGIPAEEVDATQAEFRLVPNL
                     FEIVSLDYWRINHGFVLPPAETARRRAYLATPGGRAHLHRLVDAHRPQLAAGGDEAIV
                     ESSLFGGFHDVVIARRHLVDRARYDDQLASSGRLTPEEHERYLAFTFEAARNLYAANP
                     FARDVVIFQNWLRPAGASFDHLHKQLVTVDELGASRVADIEHLGRNPHLLADYHGVLA
                     GNGLILAETDSAQLVAGVGHRFPSLEVWNLRRESDLWNIADDELADLSALVHAGHVAM
                     GARLPANEEWHYRPPTMREHLPVRVILKWRISNPAGFEGGSGIYINTIDPWTVAERTR
                     HELAAKAHRLSTRVRLLG"
     gene            59934..60377
                     /locus_tag="PKU75_00325"
     CDS             59934..60377
                     /locus_tag="PKU75_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79348.1"
                     /translation="MAAIARLRPLVVSWREEEGHPTAVTYQGTSNVTSWLAAPAAIAL
                     HEELGWERVRAHGIRLAEQGGQIVADALGTKPIPGDPVPMRLVPFECEERFAAMAAIR
                     EAHPVELAITESPGDHFVRVSAHLYNTREDYVALARACAAYVTHN"
     gene            60565..61293
                     /locus_tag="PKU75_00330"
     CDS             60565..61293
                     /locus_tag="PKU75_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005506713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79349.1"
                     /translation="MGTFDALRGRGEEAESMPGTAGLASDPADEVTDAELRDRAADYV
                     LPGFLDREEATVALRDYFELADDDPRPGQVIDQLWAARLAEQETWTERSDYQRLESAF
                     DRLGSEGVLTRMNFTCCQNCGTTEIDDERTPAPAADYPWREWAYAFFHQQDAERLAEG
                     PTVLMLSFSAFRPAADLDPELLAAAAAGDDGARRKVASETDRRVGELVVGAFRDAGLK
                     VDWNGDPRTRIAVRIEDWRKPLEV"
     gene            complement(61323..61931)
                     /locus_tag="PKU75_00335"
     CDS             complement(61323..61931)
                     /locus_tag="PKU75_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF79350.1"
                     /translation="MSEIRPYHHGDLRDALIAAGLDLTRAGGPAALTVRAATRRAGVS
                     PNAAYRHFADRAALLQAVRVAIQQRMAAGMGTMDQEADTTETAIAMLRAVGLGYIRFA
                     LTEPGWFSVAFFGEGAAQRAFGDTAWPAPFVALEAALDALVTTASLAPADREGAEWPC
                     FAAVHGFAELALRGPLRGKDTDSLMTLAERTVDTTIAGLLGR"
     gene            62004..62543
                     /locus_tag="PKU75_00340"
     CDS             62004..62543
                     /locus_tag="PKU75_00340"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HPF79351.1"
                     /translation="MEPDVVIVMGASGTGKTTVATGISTRMGWTFAEGDAFHPQANVD
                     KMRAGVPLTDKDRAPWLRRIGDWISEQVAEGESCVVTCSALKRDYRDVLREGRPQVRF
                     LHLHDDMELIAARMAARKDHFMMPTLLPSQFATLQMLGPDEPGVVVTVRDDVPTVIRN
                     SMTALGLTPPDPAGEGVLA"
     gene            complement(62655..64118)
                     /locus_tag="PKU75_00345"
     CDS             complement(62655..64118)
                     /locus_tag="PKU75_00345"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HPF79352.1"
                     /translation="MAAPSQKALLESVPTSLFIGGDWRPAEGGATLDVHDPATGRVLT
                     QVADGSPADGLAALDAAVAAQADWARVPPRDRGELLRAAYEKLTERAEEFALLMTLEM
                     GKPLAEARGEVTYGAEFFRWFSEEAVRIHGRYAVAPNGATRLVTMKQPVGPTLMITPW
                     NFPLAMGTRKIGPALAAGCTMVVKPAHETPLSMLALAQLLDDLGLPKGVLNVVTTSHS
                     GAVMEPLIRDPRVRKLTFTGSTPVGKKLVEQSSEQLLRLSMELGGNAPFVVFEDADVD
                     AAVQGAMLAKMRNIGEACTAANRFFVHTSVAEEFASKFAAAMGALTVGKGTVKGIDVG
                     PLITAKARDGVDELVQDAVAKGARALTGGAPVAGKGFFYAPTVLTDVPAGARCMTEEI
                     FGPVAPICTFTTEADAIAKANATEYGLVGYVFTKDLSRAIRVAEALEFGMVGINQGIV
                     SNPAAPFGGVKHSGFGREGGFEGIEEYLETKYVGIAL"
     gene            complement(64168..65223)
                     /gene="speB"
                     /locus_tag="PKU75_00350"
     CDS             complement(64168..65223)
                     /gene="speB"
                     /locus_tag="PKU75_00350"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPF79353.1"
                     /translation="MGRYGAQFGPDITFLGVDRCDLDDPASYAGADIVILGAPFDGGT
                     SHRPGTRFGPQAIRMTDYLPHDGSRPSLALRTDGLQDLRVLDAGDVEMYSGDIETALP
                     ALEAAVTKVVAAGAIPIVLGGDHSIAFPDAKGCANAIGQGRVSMIHFDAHADTGDIEF
                     GSLWGHGQPMRRLIESGALRGDRFLQIGLRGYWPPPETLDWMAQQNMRSYEMTEIVHR
                     GLGECLTEAFAIATDECEGVFLSVDIDVCDPGHAPGTGTPEPGGLSARELLDAVRRIC
                     LELPVCGVDVVEVSPPYDHADITAALANRVVLESLSAIARKRKDERDGTTWDPALPLL
                     SGRAADIPEGHIGGHSH"
     gene            65370..65819
                     /locus_tag="PKU75_00355"
     CDS             65370..65819
                     /locus_tag="PKU75_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79354.1"
                     /translation="MNLSDVIRPNLDNIVVAAAAVVDAAGLPHYMSLDPQGVKLRLRE
                     LAEAMLMSVETGEPVHFASHAQSIAHDRFLAGVHIEELLTTFNALETALWENVVKVAP
                     TEDVVADLGLIGSIIGAGKDQVASSYVEMATQRHTHGLDMSHLHEAL"
     gene            complement(65924..67198)
                     /locus_tag="PKU75_00360"
     CDS             complement(65924..67198)
                     /locus_tag="PKU75_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPF79355.1"
                     /translation="MRVLMIGAGGVGDAAAKIAVERDFFDVFVVADYDVERAQRTVSA
                     VQERRPGERRFHAVGVDASDAENVAAMIRLTGATHVLNAVDPRFVMPVFDGCLAAGAN
                     YLDMAMSLSNRHPDKPFAMTHVKLGDEQFAKAHDWEQAGKLALVGIGVEPGLSDVFAR
                     YAQDNLFSEIDELGTRDGANLAYFGPDGSEAFAPGFSMWTIIEECLNPPVVWEKERAE
                     KGGSLEAGFFTLPPFSEPEVFDFPEGIGPVECVQVEHEEVLLMPRWLDAKRVTFKYGL
                     GEQMISILKALNALGLDSTDPITIKGVQVAPRDIVAAVLPDPATIGPQLTGKTCAGVH
                     VTGTGKDGRPRSTYLYHVADNEVTMREYGAQCIVWQTAINPIVALELLANGTWAGSGV
                     LGPEAFQAEPFLELLAAPQPEGYGSAWGIEEK"
     gene            complement(67281..68087)
                     /locus_tag="PKU75_00365"
     CDS             complement(67281..68087)
                     /locus_tag="PKU75_00365"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF79356.1"
                     /translation="MQAWFANRFALIVAVFVLLYLFVPVAYTFVFSFNNYRKSNITWN
                     PEGSPTLKHWQNPCGAPGVCDSLVKSLEIGAIATLAATVLGTLLAFALVRYKFVGRGT
                     GNLLTFIPMATPEIVLGASLLTIFVQGFSNVGLRLGFWTIVIAHIMFCISFVVVTVKA
                     RLQSLDPRLEEAAQDLYAGPGSTFWKVTFPLVLPGIVGAAMLAFSLSFDDFIITNFVS
                     GSETTFPKFVYVSYLRGIPAQANVIGFSMFVIAVLLVVLGQVIGNARKPK"
     gene            complement(68135..69043)
                     /locus_tag="PKU75_00370"
     CDS             complement(68135..69043)
                     /locus_tag="PKU75_00370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF79357.1"
                     /translation="MSALAHIGAGQGAPAGPDTAKRGSWMPYLLLLPGMLWLIVFFVV
                     PLVQLFTVSLQSKFPGYPGYYYRDLNFKNYWSALTEFAPHFGRSLLFAGLATFFAFCL
                     AYPLAYAMAFKAGRWRGLMMICIIAPFFTSFILRTIAWRQILADEGPVAKTLNFLHVL
                     PGGHITETWMAVVAGLTYNFLPFMVLPIYASLERADPRIIEAGGDLYANGFTTFRTVT
                     LPMSMPGVLAGTLLTFIPAAGDYVNAELLGSDRSTKMVGNVIESQFFKVPGGYPTAAA
                     LSFTLMALILALVFVYVRKFGTEELL"
     gene            complement(69040..70224)
                     /locus_tag="PKU75_00375"
     CDS             complement(69040..70224)
                     /locus_tag="PKU75_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79358.1"
                     /translation="MAGFREATGDLRIENVTKEFGDFTAVDDLTLSVPRGSFFALLGP
                     SGCGKTTTLRMVAGLEQPTRGRILIGDTDLTGSRPYERPVNTVFQSYALFPHLTVRDN
                     VAFGPKRRGQKDATRMADEALELVQLPHLAARKPAQLSGGQQQRVAVARALVNRPEVL
                     LLDEPLGALDLKLRRQMQVELKRIQTEVGLTFIHVTHDQEEAMTMADTVAVMNHGRIE
                     QMGPPAQMYDLPKTAFVANFVGQSNLGHGRITGRDGDFFVADVHGSTVKIPAARSAVD
                     SGDILFGVRPEKVHLARDSHDVIDRTRKVALGDDMKATVVDVSFIGVATQYLVQVASG
                     ATWSVYEQNLDVDPIDLKPGDPVWIEWDPAHAFAVAAEGAEIPAGPVLGDPVGMPRDT
                     VS"
     gene            complement(70224..71459)
                     /locus_tag="PKU75_00380"
     CDS             complement(70224..71459)
                     /locus_tag="PKU75_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HPF79359.1"
                     /translation="MPRRIPPSDPAVRALIRSARASSLSRRSLLSASAMGAGAAALTA
                     CAPPKPPAGGVAAVKLPTDVSATEKVVNWANWTAYLDMDDEGKTYPTLEAFIKETGIK
                     ATYTEDIDDNDSYVSKVRPQLEAKQDIGKDIITLTDWMANRMIRDQLVQPLELIQMPN
                     AGNLLPPLKEVSFDPGRNYSLTWQSGFAGIGYDKERVGKELKTLDDLFTPELRGKIVV
                     LSEMRDTLGLIMQWQGVDIAGNWGKPEYDKAIEKFNQLVSDGYIRRIKGNSYLNDLKS
                     GNALAGIVWSGDLFILRAETENDNWEFTIPESGGTLWSDNMMVPITSTHRRNAETLMN
                     YYYDPKVAAEVAAWVNYVCPVVGAKQEMEKIDEELAQSPFIFPSEDYLTKNNIQGFRA
                     LTAQEDADYSAEWAKVVGN"
     gene            complement(71488..72933)
                     /locus_tag="PKU75_00385"
     CDS             complement(71488..72933)
                     /locus_tag="PKU75_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HPF79360.1"
                     /translation="MSSPRDLKNFIGNAFVDARSDARSEVINPSTGAVVASAPVSSAE
                     DVDAAYRSAATAFEEWGQTTPSERQQALLKFADAIEARAEEFIALESENTGKPHALTA
                     SEEIPPMLDQLRFFAGAARVLEGKAAGEYMKGMTSWIRREPIGVVGQVTPWNYPMMMA
                     VWKIGPALAAGNTVVLKPSDTTPETTLLLAEIASEFLPAGTFNVITGDRETGKALVEH
                     PIPGLVAITGSVRAGMQVAESASQQVKRVHLELGGKAPVVVFDDADIEAAVEGIAIAG
                     YFNAGQDCTAATRVLVAPGIHDDFVAALADYAKSSAKVGMPDDADALFGPVNNARQLE
                     HVSGFIERLPGHANVAAGGNRISSMGDGYFLEPTVLAGLRQDDEAIQNEIFGPVITVQ
                     KFTDEAEAIAFANGVDYGLASSVWTKDFGRAMRMSKALDFGCVWINTHIPLVAEMPHG
                     GYKKSGYGKDLSMYGLEDYTRIKHVMANLDS"
     gene            73132..73605
                     /locus_tag="PKU75_00390"
     CDS             73132..73605
                     /locus_tag="PKU75_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPF79361.1"
                     /translation="MPRAARSGPQRLDDVSKRIIELLQDDGRASYATIAKAVGLSEAA
                     ARQRVQRLLESGVMQIVAVTDPLHVGFTRQAMIGIRVSGELAPAADALAGLAQVAYVV
                     TTAGSFDLLCEVVCEDDDELLDLVGREIRTLPGVVSTETFVYLKLNKQHYNWGTR"
     gene            73602..75041
                     /locus_tag="PKU75_00395"
     CDS             73602..75041
                     /locus_tag="PKU75_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPF79362.1"
                     /translation="MTIYPAEVWEPSSDRHTPTGALYSQAARDHLWMHFTRHSVFEDP
                     SDGGLGHTVPIIVRGEGHRIWDDRGNEYIDGLAGLFVTNVGHGRTEIADAMAAQAREL
                     AFFPLWSYAHPRAIELAERLASHAPGDLNRVFFTTGGGEAVETAWKLAKQYFKLVGKP
                     TKHKVISRSIAYHGTPQGALAITGIPAAKIPFEPLVPGGFRVPNTNIYRAPEHLRDDE
                     KAFGRWAADRIEEAILFEGPDTVAAVFLEPVQNSGGCFPPPAGYFERVREICDQYDVL
                     LVSDEVICAFGRIGSMFACTDFGYVPDIITTAKGVTSGYAPLGVMIAHDRLFEPFRSG
                     TTLFPHGYTWGGHPVSCAAAMANLDIFEREGLTDHVKRNAPAFRATLEKLLHLPLVGD
                     VRGAGYFYGIELVKDKATRQTFDDGEAERLLRGYLSKALFDAGIYCRADDRGDPVIQL
                     APPLTIGQAEFDDIESRLRSVLSEAPDHL"
     gene            75108..76493
                     /locus_tag="PKU75_00400"
     CDS             75108..76493
                     /locus_tag="PKU75_00400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPF79363.1"
                     /translation="MSELRPAGFPLWWEGYAAPSPAAVPPAEALPSQADVVIVGGGFT
                     GLWTAYYLLRDAAHLSVLVLEAEHVGFGASGRNGGWVSALFPLGADALAARHGASPTR
                     ALLSELRHTVDQVGDVIAAEGISEAGYAKGGTLCLARNAAQVARAKADVAAGAVWDTG
                     TLWLSSPEATERLAATQILGGTWNPHCARIQPRALVEGLAAAVRARGGRIAEGARVAS
                     AAPGVVTLSDGTRIRAGHVIRATEAYTAGLAGLRPRLAPVYSLMVATEPLPHSTWAEI
                     GLAQREVFSDYRHVIVYGQRTPDDRLAFGGRSAPYHWGSAIRPGFDQDATVFALLRAA
                     LRELLPQLGDVTFTHAWGGPLGIPRDWHPSVGYDASARVGWAGGYVGDGVAATNLAGR
                     TLADLVTNRCSPLTALPWVNHRSPSWEPEPLRWLGVNAGLRLAALADAEEARTGRPAR
                     LARLLSTLIGH"
     assembly_gap    76644..76743
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            76820..77899
                     /locus_tag="PKU75_00405"
     CDS             76820..77899
                     /locus_tag="PKU75_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HPF79364.1"
                     /translation="MEVESTEELDALLAQGISPRRLRLQDLDLTGYTGLDSRTDLDEL
                     VVLGGRLSPSLDAHLRAHGAIVFPDDPDATPVRPYRAHLYTAEELYAGLVDGYAETTD
                     ALAYDWYQNSQRHPDVFFALLQAIHDDSISDALAEFVDGHAVVGVMGGHALQRGTAAY
                     AAAARLGFHLAEHGFLVVTGGGPGAMEAANLGAAAYGADQLDAALAIVAEVPSFVPDI
                     GAWADAGMRAKAVLRPVSGQPVPRSLGIPTWFYGHEPPNVFAQGIAKYFSNAIREDWL
                     LAHAAAGVVVLPGAAGTVQEIFQFATRAYYASKTPPPLVIVDSGHWRDTLPVWPLIQA
                     LAAGRAMADRVTLVDSVEDAVAYLT"
     gene            complement(77903..78787)
                     /gene="ppk2"
                     /locus_tag="PKU75_00410"
     CDS             complement(77903..78787)
                     /gene="ppk2"
                     /locus_tag="PKU75_00410"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HPF79365.1"
                     /translation="MVKKHVDLPEGADDSGEFSHRVGKAGGTGNPEDYKTDGRLKNSV
                     YEAEMERLQEQLVLLQYWIQSQGLKVLVLFEGRGSAGKGGVIKRITERTSPRTVRVVA
                     LPKPTERERTQWYFQRYVEHLPAAGEMVLFDRSWYNRSNVEWVMGFCTENEHEEFLRS
                     CPEFERMLVRSGIVVIKYWFSVSFDEQRRRFEARNAEPLKRWKLSDMDLAEHELYVKY
                     SMAKDNTFQYTDIKQAPWYVVPSDDKKAARLNCINHLLSQFSYEDVAPEVVKLPDMKQ
                     MPYVRPPIQDQTFVDQRF"
     gene            complement(78825..80009)
                     /locus_tag="PKU75_00415"
     CDS             complement(78825..80009)
                     /locus_tag="PKU75_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79366.1"
                     /translation="MRLRSVFTALAAGLLLAGCGGDPAALPQRVDNHHTPAASPAAPT
                     SDATAQMAKEVALRPGERFVEVRMAAAYTPKAPAGEGTDDYRCFLVDPELATDAVITG
                     THFLPANAELVHHVILYKVEPQDLAKAEAKDASEPGDGWTCFGGTGIGGGGASLNLDE
                     APWLGAWAPGVSERVAGDGIGTKLPTGSRIVMQIHYNLLAGQGQDRSAARLRLAKDDG
                     SYDYLQTMLLPAPVELPCRPGKTGPLCAREKAMKDLNARFGSEAKVADLLHILCGSEP
                     IGPTQTCTRPVNGTMRVRATSGHMHLLGRAISIVANAGRPDERTLLDITNWDFDNQSA
                     TALPQPVTLTSSDTLTVKCTHDQSLRDRLPALAGVPERYVAWGEGTIDEMCLGIVMFT
                     KS"
     gene            80052..80537
                     /locus_tag="PKU75_00420"
     CDS             80052..80537
                     /locus_tag="PKU75_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPF79367.1"
                     /translation="MPRLLLVHHSPTRNLQALTESVLDGTRADGLEGVEVVVRAALEA
                     NAEDVLDADAYLLGTSANFGYMSGALKHFFDSTFLQIGGALDDSGGAGGSGGATAGRP
                     FGLYVHGRYDTTGAVRSVLSIVSALGWRQAYDVLEVLGDVGEEARESAYELGATLAAQ
                     L"
     gene            80612..80920
                     /locus_tag="PKU75_00425"
     CDS             80612..80920
                     /locus_tag="PKU75_00425"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HPF79368.1"
                     /translation="MQPDVLVARRADFSQRDLPVAPLLAVEVLSPSTRRFDLHPKRAR
                     YEAAGCANFWVVDPDAPSILAWELQDGVYVKVGRASGTELFTTDVPFPVELRPADLIA
                     "
     gene            complement(80923..>81207)
                     /locus_tag="PKU75_00430"
     CDS             complement(80923..>81207)
                     /locus_tag="PKU75_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79369.1"
                     /translation="PGAEIVPVALALATYVDETGQPYDNQLGVYAPLGHVEIQSAFAK
                     AVARHTDRRLAPVFVHDGAAALAGARLTDPRADAAIVLGAAIGSGLTIAA"
BASE COUNT        12161 a        27933 c        28334 g        12579 t
ORIGIN      
        1 ggagaagtcc cgggtggagg agaagcgcat ccggccgcga cgggcgtacc ggattcggta
       61 gcgcgcggcg acgggtgggg gcggggggcc gtcgggagtg ggactcatgg tgagccaatt
      121 atcactgccc ataggctgtc gccatggtcg ccgtcgatcg ctcccgtctc gccgtcctcc
      181 tccagcagga ggccgacgca tacgccgccg cccacccccg ctcccgagag ctgtatgacg
      241 cgtcgagcaa cctcttcggg cacgtcccga tgacgtggat gaacaagtgg tccggcgggt
      301 tcccgctcta tctcgatcac gcgcagggcg cccggatcac cgatgtcgac ggccatacct
      361 atgtcgactt cgccctcggc gacaccggcg cgatggccgg ccactccccc gacccgacga
      421 tcaaggccgt caccgcacgg gtgggggatc tcggcgggct gaccacgatg ctgcccaccg
      481 cggatgccca gtgggtgggc gccgagctca cggcccgctt cacgcttccc ctgtggagct
      541 tcacgctgtc cgccaccgac gccaatcgct gggcgatccg tctggcgcgc ttggcaaccg
      601 ggcgctcgaa gatcctggtc ttcggatact gctatcacgg cagcgtcgat gagaccttcg
      661 ccgtcaccaa ccccatccac ggcggcaccg actcccggcc gggcaatgtc gcaccacccg
      721 tgccggtcga tctgacgacc cgggcggcgg tgtggaacga cctcgagtcg gtcgaggcgg
      781 ctctgcgaca tggcgacgtc gcggcgatcc tgaccgagcc ggccctgacg aacatcggca
      841 tcgtgttgcc ggagccgggg ttcctagccg ggttgcggga gttggcgaca cggtatggcg
      901 cgttgctgat gatcgacgag acccacacct tctccgccgg ctggggtggg gccacgaccg
      961 cctgggacct ggagcccgac atcttcgtga tcggcaaatc gatcggcggc ggaatccctt
     1021 gtggtgctta tggattgagt gagaacgtcg cctcccaggt cacgcaccac acagacaagg
     1081 gggacgccga catcatcgac gtcggaggcg tgggcggcac gctggccggc aacgcgctct
     1141 cgctcgcggc gatgcgggcg acgctcgggg aggtgctcac gccgcaggcg ttcgagggga
     1201 tgatcgatct cgccacggcc ttcacccagg gcgtggaggc gggcatcgcc gagttcgatc
     1261 tgccgtggtc gatcgcgcag ctgggcgcgc gcgccgaata ccgattcgcc tatcccgcac
     1321 cgcgaaccgg cgaggaggcg gccgcggcgg ccgacgacga actcgacgcc tatatgcacc
     1381 tggcgatgtg caaccgcggc attctcatga cgcccttcca caatatggcg ctgatgtgcc
     1441 cggccaccga acgtgccgac gtggatcggc atacggacgc cttccgcgag gtggtcgggg
     1501 cgctgcgcgg atgaacgacg tgacagcccc cggactcgcc gaacccttcc ttgccggcgc
     1561 cgaagtcgct gcggcactgc tcgcgcgcga ggaggtccgg gtgggctggg atgcgccaag
     1621 cgcgttgccc cgcatgacga tcgggatgct tgcctgccat ctggggcggc agttcatccg
     1681 ctcgggtcaa cttctctcgg cgccagctac ggacggggcc gacctgctgg gcagcgctcg
     1741 cgagcactat cgccgtccct cccggtcaag gccacatcgc tcgacgatcc ggccatgacc
     1801 cgcgacctcg acaccgagga cgccagcgcc ggattcgacg cgatgctgga gcgttgtctg
     1861 cccgccctcg ccgaggtcac ctcgctgctg cgctcgggga cggcgcacga tccggtgacc
     1921 atcccctggc agggttggag ccggcggcag gatgactacc tgctgacccg tctcgtcgag
     1981 atcgtcgtgc actcggacga cctggcccac tcgatcggtg cgccgacccc tgaattcccg
     2041 tccgcggcat acgatcccgt gttgcacctg ctggcggacc tggccgccga acggcacggc
     2101 cagtccgccc tcgtcagcgc cttgacccgc cgggagcgca tgcctgggac gatctccgcc
     2161 ttctgatcgg ctgccgcggg gcatagcgcg cgggctcagc tgcccggctc atgtcgcgcc
     2221 cggatcgcga ccaactcgac ggcgtgctca tcgaggcgcc ggtcttcgtc ggtgacgggc
     2281 gtccacggcg tcacgggcag cgccttgcgt tgctcgtcga tcgtgacgaa caccgtgcga
     2341 cagtaggtcg tcacctccat ctcccccgtc gccgggtcgc ccgaacggac atgcacgccg
     2401 atgtgcatcg aggtgcgacc ggtgtgcagg aggcgtgcct cgacctcgac caggtgcccg
     2461 atgcgcagcg gacgatagaa ccggacgcca ccggtgaaaa ccgcgacatt gcgcgggtcg
     2521 ccggtccagg acgtggcgag cacatgagcg gcctcgtcga tccagcgcat gacgatgccg
     2581 ccgtgcacct tgccacccca gttgacgtcg gtgggcgcgg ccaggaaccg catcgtgatg
     2641 cggggggccg tgcctgcgtc ggtgaagacc acccgcttca tggcctcctc gacctcgtgc
     2701 cgcacggcag cccggcgcag cgcccagtcc cgcagcaccc gttcggcctc acccttgggt
     2761 tccagcgcag gcaccggcct cggcttgcca tcgccgtcga cggatacgaa gaccatgagg
     2821 cagtgggtcg aggggtccaa ctccccggtc gacggcgggc cgctgaagac ctcgaccatg
     2881 acgtccatcg acgtgcgacc cgtgcggacg acccgggccg tcacctcgac gaggtgaccc
     2941 acttcgaccg gccgggtgaa gcggacgttg ccgacgtagg cggtgacggc ataggtcccg
     3001 ctccaacggg cgacggcggc atacccggcc ttgtcgatcc actccaaaac ccttccggcg
     3061 gagaccgatc cggattgacc ggcgtctgtc ggcgcggcga ggaagcgcag ggtgatcgcg
     3121 tcgtgaatgc ccgtcatcgc ggcaccgccc acgcgcgcac gcgctcgacc tggtcgggcg
     3181 tcccgaagac gatgagcgtc gaacccgcca ccagtgacgt cgaatccgga ggcgccgcaa
     3241 cgaaggcacc ctttcggggt gcgcgcaccg cgagctgctg acacccgaag cgggccggga
     3301 cgtcgaggtc tgcgacggtg agtccggcga agggtgagcc ctcggcgagc cggacctgct
     3361 ggatgcggtg tccgaagtcg tcgtcatgca gcacctcgtc gaggaactcg gcgacgtcgg
     3421 cgtgcagggc gaagctggcc atccgccggc ctccgatcag ttgggggttg accgcccgtg
     3481 tggcacccgc gagcacgagc ttctccttgc tgtcgagcgt ccgggcgcgc gagatgatca
     3541 cgaggtcggg ccgcatactg cgggcggaca gggtcgcata ggtcgtctcg gcatcggtgt
     3601 ccagcgcaca gatcagcgcg cgcgcctgca tgatgcccgc ctcctggagc acctcgttca
     3661 cgctcgcgtc gcccagcacg tgcggcaggt ccgtgcccgc gaaacgcgtg tcgtcccggt
     3721 cgaccagcgc gacctgattg ccggtatcga ggagatgagc ggtcgcggac tggccggcga
     3781 gaatcatgta gcccacggtg ccgaagacgg tgacagcgat caggccggca atggcgagct
     3841 tcatccggcg cagggcgtcc gcgccttcag gcacgcctga caacagcatg cggcgcctgt
     3901 agctcgccga cgaccgtgtc acaggggcat ccttcaccac cgtgccccgg cacgtcacgt
     3961 agggtcctgg gatatgacga ccttgagcga gactctgctg actcccgagc gccgccccca
     4021 gaccgtcgat gccctcgtcg gcgtcgtgga ggacgaggtg gcgagcaagt ccggactgag
     4081 cgggacagcg atcaagacgg catacaaggc cgcccgcaag atcgacgacc ggatcgtgcg
     4141 ccgggcgatt gcggggatgc tgccggagtt cctcgaccag ctcgagccct tctgggccgg
     4201 gcgaggcgag cagccgttcg gcgcgcacct ggcgggccag ggcgagcggg cctccgaggc
     4261 cctgcttgcc gtcaccgacg ctcgcgcagc caaccccaag cacgcggcgg tcgccaaggt
     4321 ctacgggatg ctgcgcggca aggccaaaga ccatgtcccc gccgccctcc cccgcgtcgg
     4381 cacggctctc gagaggcaga tgtcctgacg gtctgtcatg gacgacgtca tggaggcgtt
     4441 ccgcgcggtc ccgcgcgcgg aattcctccg tgccggtgac cggcggcgcg cggcatacga
     4501 cgggccgatc gcgatcggcc atggccagac caacagccag ccccgcaccg tggcggacat
     4561 gctgcgtctg ctcgatgtgc gggccggtca tcgcgtcctc gacgttggcg ctggatcggg
     4621 ttggacgaca gcgctactcg cacatctcgt cgggcctcag ggtctcgtca tcggggtcga
     4681 gcgggtggcc gagatcgccg agttcggcgc gaccaacctc gcggccgccg gtatgccgtg
     4741 ggctcgcctc cgcctcgccg agccggatgt tctcggcgcg ccggacgagg ggccgtatga
     4801 ccgcatactc gtatcggcgc agccaaaagt gttgccgcag agtctgatcg accagctgcg
     4861 gatgggtggg gtcatggtga tccccgtggg cgcccggatg ctccgcgtgg tgcggacgac
     4921 cgatggccac aacatctcga cccacggcta ctaccgcttc gtgccgcttg tcacgaacga
     4981 ctgaactctc gccacgaccg agcggacctc agaggtcgag acgcatcaga tccgtcgcgg
     5041 gacgcagacc gagcgacgca tagagcggga tggacatctc gctgggggcc aatacgattc
     5101 ggaccatctc ctccgatcgg gcccagtcga tcgcggccgc catgagggct cgaccgatgc
     5161 cctggcggcg gtgatacggg tccacccaga cgttcgccac gtagagccag cgggccgggg
     5221 gccggccggg ccgcggcatc cgggtgaaga ccgcggcgtt ggccatcccc acggcatacc
     5281 cgtcgacgcg agccagccag ccgacccgat tctcgctcgc ccaccactgg cgaatgccgg
     5341 cgacgaagtc ggcatcgggg ccggccggct cgcccgacgt ccagtgctcg cgcaagcggg
     5401 cgacatcggg cagatcggcg tccgtgatcg gggcgatctg tggcgagttc atgagcgagt
     5461 gacgctgagc ggcaacagct ctcggcccgt gggcccgatc tggatgtggg tgccgagctg
     5521 tgggcagacg ccgcagtcga agcagggagt ccagcggcag tcgtcctgtt cggtctcgtc
     5581 gagggcgtcc tgccagtcgg cccagagcca gtccttgtcg aggccggagt cgatgtggtc
     5641 ccagggcagc acttcggtct gaccgcgttc gcgggtggtg taccaggcga catccacgcc
     5701 gtacgccggg agttcggcct cgcacgcggc gatccagttg tcgtacgaga agtgctcgcg
     5761 ccagccgtcg aagcggccgc cctcgcgcca cacgcgctcg atgacccggc cgacgcggcg
     5821 atcgccgcgg gaaagcaggc cttcgatgat gccgggcttg ccgtcgtggt agcggaagcc
     5881 gatggcgctg gcgtacttgc gctcggcgcg gatcgcctcg cgcagtttag cgagccgtgc
     5941 gtccgtctcg tcggcgccga gctgggcggc ccattggaag ggagtatgcg gcttcggcac
     6001 gaacccgccg atggagacgg tgcagcggat gtccttgcgc ccgctcacct ttcggccggt
     6061 gtcgatgacc ttcttggcga ggtcggcgat ctggaggacg tcctcgtcgg tctcggtcgg
     6121 cagcccgcac atgaagtaga gcttgacctg ccgccaccct gcgccgtatg cagcggcgac
     6181 ggtgtcgatg aggtcctgct cggtgaccat cttgttgatc accttgcgca ggcgctcgct
     6241 gccgccttcg ggggcgaagg tgagtccgga gcggcgcccg ttgcgggtca gctcgttggc
     6301 gaggtcgata ttgaacgcgt ccacgcgcgt cgaggggagg gacaggccga cctgctcccc
     6361 ctgataccga tcggccaagc ccttggtgat gccggcgatc tcggagtgat ccgcgctact
     6421 gagagagagc aggccgacct cctcgaaacc cgttgcggcc aaaccgcgtt cgaccatgtc
     6481 gccgacgccg gtgatcgagc gctcgcggac cgggcgggtg atcatcccgg cttggcagaa
     6541 ccggcagccg cgggtgcagc cgcggaagat ctccacgctc atgcgttcgt ggacggcctc
     6601 ggcgagcggg accagtggct gcttcgggta aggccactcg tcgaggtcca tgacggtgtt
     6661 cttcgcgatg cgccacggca cgcccggttc ggtgggcgcg acgcggcgga tgcggccgtc
     6721 gggcaggtag gtgacgtcgt agaagctcgg gacgtagacg ttgtgggtgt gggcgagccg
     6781 gcgcagcagc tcttcgcgac cgcccgaccg gccctcggcc ttccagtcgg tgacgacgtc
     6841 ggtgacctgc agcacggcct gctccccgtc gccgacgatc gcggcgtcga tgaagtcggc
     6901 gatcggttcg gggttgaagg ccgcgtgccc gccgacgatg acgatcggct cgtcctcgcc
     6961 gcgctcggcg gcgtggaggg tgatgccggc caggtcgagg gcgttgagga agttggtgta
     7021 gccgagttcg gtcgagaagg agatgccgaa gacgtcgaac tcgcgcacgg cgcggtggcc
     7081 gtccacggtg acttgcggca ccccctcggc ccgcatgagg gcctccatat cgggccacac
     7141 ggcatacgtc cgttcagcca gcgcgtccgg gcgttcgttc aggacttcgt agaggatcat
     7201 cacgccttgg ttgggcaccc cgacctcgta ggcgtcggga tacatcagtg cccagcggac
     7261 cgtcaggtcc tcgccccccg gcccgtgtcc gccgcagtcc cagtccttga ccgtggagtt
     7321 gagttcgccg ccgacatact ggatcggctt gctcacgcgc tcgagcaacg gctcgagggc
     7381 ggcgaagcgg gactgtccgg gcatccgaca aggataggtg accctgactt ccacgcagac
     7441 ctactcatcg cccagacggc cgcgctcggc gagcgccgcc ccgttgcgag atcaaggacc
     7501 atctcgtagc cattgagggc gacattgatc gaacgctcca catggggccc gtgctgacgt
     7561 aaagcctctt gccaggtcgg agtctgctcg gcgatggtgc gcgtgaaccc gcggttcgtc
     7621 gagtagagcc cctcgatggc gccggtgtcg accgcggcat accgggtggc gacctgcacg
     7681 gcggcggcca tcgcctcggg tgtcgcgaca gcccgtgcct cgtccaaccg gttggcgtac
     7741 gcatcgacgg tggtggagtc gaagccgtcc gcccagccga caaacccagg gaagggttca
     7801 tagacgaccg acgacctgtc acgcacggca cgagcgtcat cagccgttcc gacaaacgcg
     7861 ggcgcgaagg cacccgcggc gcgcgcctgg gattcacccc acgccccaag cgagctagaa
     7921 aggcggggcg tcgaaggtgg gtagggcggt ggttcggacg atcacgcgtg gggtgtcctc
     7981 gtcgcaaggg tggtagtaag taatcgggcc gtgctgaacg gcgtaccagc agtggcgatt
     8041 gcggaggcga cggcggcggg atttcggccg ggtcttcacg tgggtgttca ggtcgtgttc
     8101 gaagacgtat tcgagcacgc tgaaccggtc acgatagggg tccgcgagcg ggcctggctg
     8161 agcaggctcg accgggccgg ccgcaccggg tgcgggcgcg ccggccttcg ccccgctgct
     8221 ggcctcgcca gggtcgggat cgccggccgc gggggagtcg ggtacggcgg gcaggatcag
     8281 cggggtaagc cggtcgagtg ggtaggtgat gtggtcccgc ccggtcgggt cggtccaggt
     8341 cgctaccgct cccggtccga ggcgcacgcg ccagccgggg gcctgtttga tccggtggtg
     8401 gcgccggcac agggcgagca ggttgacttc gctggtctgg ccggtgggcc agggccggac
     8461 gtggtcgagg tcgcagtagc gcaccgagat ggtgcagccg gggaagcggc agcggccgtc
     8521 gcggtccttg accagccggg tcagggcggc cgagggccgg tatgcggcgc tgtccaggcg
     8581 ccggtcgggg tcgctcaagg aacccgtggc cggtgcgcag gcggcgggtt cggtccggaa
     8641 ccgcaccgcg gggttgcggg tcagccaggc tcgggaggcc aggaccggtt cgccccggcc
     8701 ggggcccatc acctccacca gcgcatccgg gatcccagca cagctcgcgt ccctggtctc
     8761 cggacgcggc tgccgccaga acgccagacc gcccgagcgc tcgccggccg tagctgtggc
     8821 ggccggctcg cccgttgagt catcggggcg ggggttctcg tccgccgcgg gcatctcgtg
     8881 cgagtcgcca ccgctgtccg ggattgggga ttcctcgtcg tcgtcggtgt cgcaggaggg
     8941 cacgggcgag tccgctggag gccggccccc gtcaccgcca tcctccccgc cgttgcctgg
     9001 gttgtcgtcg tcgccgtagt tgtcgtcgtc gccggagttg tcgtcgtcgc cggagttgtc
     9061 gtcgccggcg gaatcgtcgt cgtccggctt gccgccgtcg ccgacgttgt cgccaacggc
     9121 gggatgttcg ctgtggccgt cgccggggtt gttgtcgtcg tcgccctcgc cgccgtcgcc
     9181 gaagttgtcg ccagcggcgg ggttttcgct gccgaggccg tcgccggggt cgtcgtcggc
     9241 ggggttgtca ttgctgaggt cgtcatggag ggcgtcggcg gggacggtga gaccgagagt
     9301 gacgttgatg gtcgcgttgc cggtgacgag gtcgatcagg gcgtcggcgc gggcggcttc
     9361 aataccgcct gggtggccgg cggcgacata gtcgtgggcc aaggcgtcaa cggcggccca
     9421 cgcggtcatg gcctgctcgg cggggaagga ccccatccag gtgtccacgc cgggttcccc
     9481 gggccagcgc cgcagcgacc gcgccttccg ggcccgctcg gcccgagccc gcaccaggtc
     9541 cggcgcgatc cggttcaaca ccgtccgcgc ggcccgcgcc agctgcatgg ccgtgccgga
     9601 ttgcatacgc gactccagtt cggcgatcac ctgttccgcc acctctgccg gcgcgtcgac
     9661 cagttcggcc gcgacccggg cggcccggta gccgtccagc cgacccgcgg ccatcgccgc
     9721 ggccaatccc gacaaacccg acggttgccc cggccccacc tccgggtcgg gctcgaacgg
     9781 gccgggcgtg tcatcggcat actgcgggcg ccggtccgcg gcgagtcgca ccgcggtatc
     9841 gacccggccg gtcacgaagg accgcgccac acacaaccgg gtccccacca gatccggccc
     9901 atccacggag atgtgcccca acgcccggtg gtactccacg tccgcgccgt cctcatccat
     9961 ggtggcgtcg atacacaccg tccgcacgat cgcctggtcc tgcgccgcag tcaccgcgtt
    10021 caccacggcc tgggactgtt cgacaagggc cagccactca gcccggtcgg cggcggtcga
    10081 cgggcgcgca cacaacccct gcagcgcggt gacacccgcg cgcagctgcg tcagcacaac
    10141 tgcggcgtcc gtgccgtcgt cgaaccgtgt catcgctgcc gcgaccccct ttatctgctc
    10201 ccccagcata ccgaacacca gtgcgacaat tcaacccgta tcgcaagaaa aatcgttgct
    10261 agacaatagt ttttcttccg aaggcacgcc agcacgcgag gcaccgacga cccgtgtcga
    10321 cgtctcggca ctcaccctcc cagagcccac cgacaatccc tcccaggaac taccggccgc
    10381 ttgcgcgtta cacccccgcc gatgccggac aggtaggggt atgccggcga gccccgagga
    10441 gagtttccgc agtctgtatg cggcgaccta tcccgatgtg ctggcgttcg cgcgccggcg
    10501 tgtagggatc acgacggcca cgacatcgcc gccgaggtgt atgccatggc ctggcgccgc
    10561 ctgggcgagg ttccggtcga cgacgcacgc ccatagttgt tcgccgtggc ccgcaaggtg
    10621 ctgctcaaca ccgcgaggag ccagcgccgt aggggcgcgg tggccgtgcg gatcgcctcg
    10681 caaccggatg ccggcgtcgc cggcgcgttg gacgacctcg cggtagcccg agcggacctc
    10741 acccgggcct gggcacagct cagcgcggac gagcagtaaa ccctctcccc gacgatctgg
    10801 gagcggctcg acgccgtcgc gggcaggctt ctcgagattc agggactcgt cggtccgcgg
    10861 gtgagggaac tgtccctcga actcgcctcc ggccgccgca ttcgcgtcgt cgtcgtgggc
    10921 gacgccttct acgcctgggt cgccgacatc gctcccgccg acgcccgcga cgacgccatc
    10981 gcgcacatca ccctcgttga cgggtccgcc cgcacccccg tcctgacgga gtgggccgga
    11041 agccgcggcc gcgcggcctg ccctacgacc cccggttcgt cccggcagtg atcgaaggtg
    11101 cgcaagcgga gcgggcgcgc atggtctgca cgaccaagtt cgcggagagc ccggaacagc
    11161 cggccatccc caaggacgcg ccggcgctgg ccgtcgagaa gcgggacggg ctgttcatgg
    11221 tgctcgtcgg cgacgagtcg agcgctgggt tgtgcctgtt cgacaaggcg acgctggcag
    11281 accccaccaa gccggctgcc tgggatccgg tccagctcac gtccggcgtg accccgcccg
    11341 tgcagatcgg cccacgcgac atcggcgacg tcagcggtgc cggctacggc aggtggggcg
    11401 acccaggcga ggagatgacc tggtcggtcg ccgctgtcga ggggtatgcc ggggcggaag
    11461 tgcgcggcat cgcggccgtg acgccccgcg ggcgactgac cgcgctgatg atcggacgct
    11521 ggttcaccat ctggtggccg agcatcaccc acgctggccg gctgacctac gaactcctct
    11581 tggcggacgg ctcggtcgtc accgtcacga ggtagccggc gggagcaagc ctcgcggctg
    11641 ggacgcccca tcggctaggt ccggctgcgg acggtgcggc ggcggacgag tgcggctggg
    11701 agagccgcac ccggccacgc ctcagttgta gtgggcgtcc agctcggcct tgtagcgctc
    11761 gcccacgacc ttgcgcttga ccttcagcga aggcgtcagc tcgccgctct cgaccgagaa
    11821 gtccgacccc aggatggtga acttcttgat ctgctcccac cggttgaggg tcccgttgag
    11881 ttcgtcgata tagccctgga tcatctcccg ggcctgatcg gaggaagcga tctcctcgta
    11941 ggacttgccc tcgagcccgt gctgcgcggc ccagccggtg atggcgtccg ggtcgagcgt
    12001 caccagcgcg gagacaaagt tgcgatcgtt gccgtgcacg acgatctgcg agatgtaggg
    12061 gcacatgccc ttgaactgcg actcgatcag ggacggcgcg acatatttgc cgttggaggt
    12121 cttgaacagg tctttcttgc ggtccgtcag ccggatgaag ccgcgctcgt cgatctcgcc
    12181 gatgtcgccg gtgtgcagcc agccgtccgg gtccagggcc tcggccgtgg cgtcggggtt
    12241 gttgtggtag ccctgcatga cgcccgggcc gcgcatgagg acctcgccgt cctcggcgat
    12301 cttgaactcc atgcccggca gcgcccagcc gaccgtgccg tactgccatg ccaccatgcg
    12361 gttgaccgtc gaggctgccg aggactcggt catgccgtaa ccctcggcga tcttcaagcc
    12421 catgccgtcg aaccagcggc ccacgtcctt gttgagcgcg gcggagccgg agatgaagaa
    12481 gcggatattg ccgccgaagc gctcgcggat cttgctcatc accaacttgt ccgcgatctt
    12541 gtgctgggcg cccagaagcg ccgacggcga tttgccctgc tcgcgcagct cgctgacctg
    12601 tgcgccgacg ctgccggccc agttgaagag cttgagcttg agcccgccct cctcctccat
    12661 catcatcgcg atcttgccgt gcgccttctc gaagatgcgc ggcgcggcgc ccatgaaggt
    12721 aggtttgacg acggcgaggt tgtcgacgat cttgtcgatg cgcccgtcga tcgcggtggg
    12781 gaagccgatc tgcaacggga tggtcagcag catcttgccg aagacgtgcg acagcggcag
    12841 ccagaggtat tggaggtcat ccgggccgag gatccggatg gcgtcgaccg aggcgccgat
    12901 ataggtccag ttgctgtgcg gcagccgcac acccttgggc cgcccggtcg tgccggaggt
    12961 gtagatcaag gtcgagagcc ggtccggggt cagattgtcg atccgtccgt cgacgacccc
    13021 ggggttgcgc tccaactcct ggcggcccag gatctcgaga tcctccaggc tcatcaccca
    13081 gtcgccgtcg ccgtcgccgt cgaagaccac gaccttgctc acgctcggga tgacatcgcg
    13141 gatcgagcgc agcttctcga gctggtcctc gttctcggcg aagacgacct tgctgtcgct
    13201 gtcggagatg atgaaggcaa cgtcgtcggc gatcgtcgtg gggtagatcg tcgtcgtcgc
    13261 cgccccggcg cacatattcg ccaggtcggc cagcgcccac tcgtagcgcg ttccggaggc
    13321 gatcgcgacg cgttcctcct cgccgacacc cagcgcgatc aggccggccg ccatcgcata
    13381 cgcgtggtcc ttgacctcgc gccacgggac cttgacgtaa tcgtcgcctt cggcatacca
    13441 gaaaccgatg gcgtccggag tcttggcgac acgctcacgg aagaggtgcc cgacgctttg
    13501 cgcctggttc tccaggagtg agctgtcgat gtcttcttcg gtcgggtggt tcacctgggt
    13561 gggcatcctt gtccacatct ttcgttctct gccggtcgga aggtggcgcc atagtgacat
    13621 gcatcggcgc cgatgtctgc ccctgatgag tattcttccc gtcacgattc ggccttatca
    13681 tcggcgccta tggctggcgg tgcgttctcg agcacgcgcc gtcgggtttc ccggaccgtg
    13741 ccggcggggg cgcgcagggg ccgcgtcctg ctgccgtcgc tgatcctcgt ggccacgctt
    13801 gcctctgcgc ccttcggcgg gctgcgtgcg accgaggccc agccgccggc cgcgctcaag
    13861 gcgggcgagg tgatcgccac gggccccttc gatgtcacgc tcggcaaggt cgtcaccctc
    13921 gatgagctgt cggtcaacga cctggcactg ccccccgcga gccccggcgg ccgcctgatg
    13981 gccatcgaac tgacggtgac caacaccaca gacgagccgc tgtccccgct gcgcatcacg
    14041 gacgcgatcg gcgggccacg cgcggccgtg gagccgtggc cgggacagga cgaacctcac
    14101 ggcgaactcg tcgccgtggc cgacggcacc cgcgtacgca ccgtcaaccc cggcctgagt
    14161 gtgcaagccg tcctggtcta tccgcagccg gcctcctgga ccgccgaccg catcgacctg
    14221 gacgtctacg acttccgctt ccaggaagag gacccgctct ctctcgatcc cgacacctgg
    14281 ctcctgcact ctgtggtcgc gtccggcgag ttcccgatca aggagtcggc gtgagacgcc
    14341 tgacccggat cgccgccgtc gtcgccaccc tggccgccgg ccacggaatc accacgctgc
    14401 tgcccaccga caaggtcgca gcggctccgt tcgtcaccgc cgccgcggtg ggcgagcacg
    14461 tgcgcctgcc gtatgccgag gtccgcctcg caggcatcca cctcgccacg aaggtgcaag
    14521 ggcagcccac gaccgccact gcatacggcc gtttcctcgt gctggacctc gaagtgacgg
    14581 cgctgcggga gccgctgctg ctcgcggcgc cctacctgcg tgatggcgaa ggccgccggt
    14641 atgccgcgga taccggcgcg cagtgcgcca ccgctgtcac gctgcccacg gggctgccgc
    14701 gctacgcgcg catgtgcatc gacgtcccgc ggcgcgccct cgatggggcc acggtcgtga
    14761 tcgcgcgggg cgcgatcgag atcgacgggt atcgccgcga cgcggttgcc gagatcgagt
    14821 tgtcgctgga ttccactgcg gcggaacagc tctggaacac ggcggcaccg gtcaccggct
    14881 tcctgtccgg cccgacccca cccggcgact gggcacgagc atgagcggtt ggtggcgcgc
    14941 caaccgcgcc tggcttgcgg cgctgccggt cgccgccgcc gtcggggcgg gcgcttcggc
    15001 ttatcaggtg cgcaccttct ggtgggagtc cggaatgcac cggcaggtcg ccgcacccgc
    15061 gccgggcgag ccggccggcc tgacggagcg ctaccgcgac gggcaaggcg agaccagccg
    15121 caccctcagc gtccgtttgc tcgggatcga ccccgcgccc cccgacccca cgcctcccga
    15181 ccccgcgcct cccgacccag gacccgccgg cggggcaccg ggcgcggcgg cataccgggt
    15241 ccggctcgag ttctcggcgc gccccgacca ggaactgcgt ccctgccgca tcgcggtgct
    15301 gggggcggac ggctcccggt atgaggcgga gccgggcctc tcgccctgcc tcccggagga
    15361 tcatcccggg ccgtcggcgt cggtgatcga gggcgacccg cgcggacacg tcgagccagg
    15421 tgaggaacgg ccggccacct ggcaggtcgc gccggtgatc caaaccgcgg ccgcggcgcg
    15481 gatcgtcgcc gtgcgggtct ggttcggcta tccggagtat gtggagttgc gcgccgagcc
    15541 ctgaacctca gcgccctgaa cctcaccgcc ctgaaccgca gccgtcgtcg ctgcccgatc
    15601 cagtacccaa gccacggtgg ccgcgaggag cggcgccaac accgtgagcg agagcgcgag
    15661 gccggccgcc ccttcgatcg gggagaacgc gagccaggtg tcggtcttga ccggcccgac
    15721 gacggcgcgg acggcatacg agacgagcgt ggggatccgc acgatcagca acatgatcag
    15781 acacaggccc aacatgggga tcagcccgat cgccccgagc acgcgcaccg cgtccgcgaa
    15841 gggcccgaac cgttcgagga tgtcgtcggc gaaggaccgg acaacgggcc agcgcaacca
    15901 ccgcgcccgc tgcccctccg ccgcttcgtg ctcggggaat acgttgtgcc ccagcacgat
    15961 cgtggcaacg acgagccagg cgatggggac caggaggacc gcctccgcgg agccgaggag
    16021 cgcgccggcc agaccaccga cgacggcgag accgtcggcg agccaggagg cgtgggccgt
    16081 gacccagtcc cacaactgcg cgggcgagga tgaggcgcgg cgggtggcga gccagtcgag
    16141 tccgccggtg cgcagcatcg ccgactgcac cgcgagttgg ccggtgtagt agacctcggc
    16201 gagcgccccg agcagcccga gcgcgaccca ccccgtgcgc gcctccgcga tgccgagcgc
    16261 ccaccgggcc acccacgcac ccacgacgat cgcgagcgcg atgggccagg catacagccc
    16321 caggcggccc atgaagtcgt agtccacctc ccgcgtcagg tcgaactggt tgaactcgtc
    16381 gaaggctgcg acattggtga accgcgccaa gtcctgatcg aacagcccgt aggcgatata
    16441 ggccccgagg aaggggatga gcaccgaggc ggcgatatcg agcaggcggg tcgggcggcc
    16501 ttcggtcgtg gcccgcggag cagtggcgtc gcgggctgcg cgcaggcgcg ggagcgtgcc
    16561 acggcatacc cgcagcatcc cgacgaccgc gaggaggtaa ccgaccggcg ccagggcgag
    16621 aagccattgg gccgcaatcc cgctgtgatc gctgacgatg acggcgagcc acacggctcc
    16681 cgcccgccag gcggccccga gcagcccgag cgtcagcagc gcgggccagt ggcgcagcag
    16741 gagacgtccg gtgtcacggc cgatccggcc gagttctcgc cccgctgctt ccacgggcgg
    16801 tgagtctgcc agacagcggg ctctgccaga cagcgggcga ctcagacgcg gtggtcgagc
    16861 accgcgtggg cggcgacgac atcgccggcc atggcgtcga gcaactcctc gatcgaatcg
    16921 aaccgcagcg tcggacgcag gtgctcgacg aagtcgacgg ccacggtctc gccatagaga
    16981 tcgaggtcgg tgcgatcgag gcagtaggcc tcgacggtcc gttcggtgcc gtcgaaggtg
    17041 ggattggtgc ccacggagat ggccgcgggg aggcggtggt ccgggtgatc cggcgggagg
    17101 tccagccggg tgaaccagcc ggcatacaca ccgtcggccg gcacgacgcc gcgcgacctc
    17161 cgctcgagat tggctgtggg ataacccaat tcgcggcccc ggtggaagcc gtggacgacg
    17221 accccgacga cccggtgcgg ccggccgagg atctccgcgg ccccggcgac atcgccctca
    17281 cgcagccgct cgcgggccgc gctggaggac cagcgcgcgg cgttcccctg gtcggggacg
    17341 acgacgacgt cgaagccgtg ccgctggccg agttcgcgga tcaggtcgac atcaccggtg
    17401 taaccgcgcc cgaagccctt ggtgtcggcg ccgacgatca gagccttgac gccgagaccg
    17461 gtgacgaacg tcgagaggat gaactcctcg gggctctgct gggcgtagtc gacggtgaac
    17521 ggcacgacga ccaagccgtc gatgccggtc tcctccagca gttgttcgtg cagtgagccc
    17581 gggctgatca gctcgggcgc ccgctcgggg tgcagcacct ggatcgggtg cgggtcgaag
    17641 gtcacggcca cggccggcag accacgcgcc ttggcctcgg cgagcagggt gccgatgacg
    17701 acgcggtgac cgcgatgcac accatcgaag ttgccgaaag tggccacgca cggtcgcaag
    17761 gcgtcgggcg tggcggcggg atcggtccag cggagcacga gggtcactct acgaagccgc
    17821 gggcgcgaag acgacatgag gcttggcccg tggaccggat tcgtccagca tggcgacgaa
    17881 ccggccgtcc gggccgaccg ccgccaccgg ctcgcggcgg cccggctgcg ccgatgggat
    17941 gcgctggccg tagccgatgg cgcgcgcctc gtcggcggtc agggttcgcc tggtgaaggc
    18001 ctgggcggtg gcgtccagcg gctggatcgg cagctcgccc gggccgtggg tctccaggtc
    18061 ggagagcggg tgggcgacgt cgatggtgaa ggcgccgacg cgggtgcgac gcagcgcggt
    18121 cagatggccg ccgacgccga gtgcggcgcc gaggtcgcgc gccaacgcgc gcacataggt
    18181 gccggaggag acgtccacct cgacgtcgac gtcgagcatg ggtatcccgt ccgcctccac
    18241 cgaccgccgg tcgcgaatct cgaagcggga gaccgtgatc ggtcgtgcgg ccagcgcgac
    18301 cgtctcacca ccgcggaccc gggcatagga gcggacgccg tcgatcttga tcgcgctcac
    18361 agcgctcggc acctgctgga tctcgccggt gagaccggca accgccgcct ccaacgctcc
    18421 tggttgcagg tcgaccggtg tcgcctcggt gatctccccc tcggcgtcgt cggtgacggt
    18481 gctggcaccc aggcggatcg tcgcggcata cgacttgtcg cagccgacga ggaaggtgag
    18541 cagcttggtg gcgcggccga tgccgaggac gagcacgccg gtggccatcg ggtcgagggt
    18601 cccggcgtgc ccgaccttgc gagtggccgc caggtagcgc accttggcga cgacgtcgtg
    18661 gctggtccag ccgccgggct tgtcgacgat gagcaggccg tcgggggccg gctcgcggcg
    18721 tggcgcccgg gtgtctgtgc ccacgaggcg ctacgagcgg gagatctcgg cgtccgccgc
    18781 agcgtcggca gcgtcgtcgt cgtcctcgtc gccgtgcttg taggggtcgg actccccggc
    18841 gtatgccgca ccggccgccg tggcggcgac ctccgcgtcg cgggccttgg cctcacggag
    18901 cagaccgtcg atatgggcgg cgctgtcggg cagcgcgtcg gcgaggaact ccaagctcgg
    18961 cgtcagccgg atgccaaggt gtttgccgac gaggctgcgc agcttgccgg tgttctgctg
    19021 caacagtttt gcggtctgct ctgcctgatt ctcgtcgccg aagacggtgt agaagatgct
    19081 cgcatgctgc aggtcgccgg tcacccggac gtcggtgatg gtgacgaaac cgaggctcgg
    19141 gtccttgacg gtggcatcga ggttgcccgc gatgacgtcc ttgatccgtt cggcgacctt
    19201 gcgagctcgt gcggtgtcga ccacggtcgc gcctccttca ctctcggcgg gaaaagctgg
    19261 gcagcgccca ccatatgtca gggtcacccg tccggcgcgc gagagaatag gccggcagcc
    19321 acccgaataa tcactggcgg cgcccctctt gggacggcat cctggtcagc cggcgcggaa
    19381 gcggccgagg tggcgtgcca cgatctcggt tccgcgtatc gccgcccgac ccatgcactg
    19441 caaatgcatc caggtcgcca ctccaaaggt ggggttcgaa tccccaggac cggccgccgg
    19501 tggcacccgg cgcgcccggc gctctgtggt gccgcttcgc ccctcggggc cgggcggcga
    19561 accccgcgcc gggccgccgc caccggcgga ccggccaccc cggcgcacaa ctgcataccc
    19621 caaaccgctc cggcactgag tcgttccacc gaactgatcc atccaccgaa ctgatccgaa
    19681 ggaggtcagc ccatgtccac catcaccatc gctccccgcc aggtcgccat cgagaccaag
    19741 gacggcctgg tcacccgcac cctgacctcg ggaaagcacc gccggatccg cggcgcgacc
    19801 tacacgatcg tggatctgcg cgagcagctg ctcaccgtgg cgccgcagga ggtcctgacc
    19861 gccgacggcg cgcaggtccg ggtctccgca gccgtgcggt gggcggtcgg cgacccgatc
    19921 gcgttccacg agaaggccgt cgagccgctc tccagcgtct acctggccac ccaggtagcc
    19981 ctgcgggatg ccctcggcgc cgtcgggatg gacgccctgg cccagcgcgg tgcccgcctg
    20041 cccgtcgccg agatcaccgc cgcggtcgcg gccgtcggac tcgaggtcgg catccgcgtc
    20101 gtcgaggtga tcgtcaagga cgtgattctc cccgcggagc tgcgagcggc cgcgacggcc
    20161 gtgctcgcgg ccaagcagcg tggcctcgcc cagctcgagg ccgcccgcgc cgagacggcg
    20221 gcgctgcgct cgctggcgaa cggcgcccag ctgctcgacc ggcacccggc cctggcgacg
    20281 ctgcgcgcga tccagtcggc accgccgggc acccaactcg tgctgcgcct cggcgggggc
    20341 gaggagcggg cacccggaca gtgacatagc gcagtgacat agcgagtgac atagcgcagt
    20401 gacatcgccc ggcgggtccg ggctgcctcg ggtggggcgt ggtcgcctgg gccggcgacg
    20461 acgtcccacc cgacgcggac ccagataccc cgggcagtac cctggaggag tgatcgtggc
    20521 tgggggtgtc gtcgggcgga gcgtgcgtgc tgctgccctg acggccctcg ccctcggcgt
    20581 gtccggcctc gcgcacagcg ccggcggtag gccgctgagc ctgcccggcg tgctcgtcgc
    20641 cctgtgcgcg ctcggccccg tgacgtgggc ggtgagcgct tctcgcctgc ggctgggccg
    20701 actggtggtg gtgctcggcg cgggacaggc gctggcacac atctctttcc tcatcgcgca
    20761 cgggacgagc gctgcttccg ccctgagcca cgctgctgct ccgatgtccg ggctgccggg
    20821 cccggcgttg tcgggcacgg cgttgtcggg cacggcatcg tcgggcacgg cccacggtgg
    20881 ccacgtcgca gcgctgtccg ggccggcgct gctgccatcg ggccggatgc tgctcgcgca
    20941 tggcgtcgcc accgtggtcc tggcgcttct cctcgcgggg ggagaggcgg tgctgtggcg
    21001 ggtgctcggc gccctgaccc ggctgcccgc ggcggtctcg gtccccccgt tcgcccgcct
    21061 ctccggcata cctgaccgcc tgcacatcgc ccctccggcc ctgtgggccg acgcccgcgc
    21121 gatgcgcggg cccccgggac gcgtgaccgc ctgaccgtgc cctgggcgcg gtcacccggc
    21181 acctcactca cccggcacct cactcacgac cagcacgtgc tgctcggcct cgaccgcgct
    21241 ggaatcgttc cggcggggca cctccatacc tcacgtccct gacgcgccca cggcgcgacc
    21301 tcgaaaggca acaaccgaat catgatctct cgcaaccgtt cacgactcgc ggcggcgtcg
    21361 gccgccctgc tcctgtccac ctcactggct gcgtgcggct ccggcgacag ttccagttcg
    21421 tcctcccctg gcacatccgc ggcgtcctcg accgcagctg gttccgccgc gacgtcggcc
    21481 agtgactccg gcacgccggc ttccaccgaa tcggcatcct ccggatcgac ggggcggatc
    21541 ggcccggtca ccatcaccga cggatgggcc aaggccaccg acatggagat gagcgccgtc
    21601 ttcggcaccc tcgtcaacaa cagcgacaag gcgatccaca tcaccggcgg atcgtccgat
    21661 gtcgcgggat cggtcgagac gcacgagttc gtcaaggacg ccggcgggaa catggtcatg
    21721 cagaaggccc cgaacgggtt cacgatcgag gccggcaaga ccttcgtcct ggcacctggt
    21781 gccaaccaca tcatgctcct cgggctgaag gacaaactcg tcgccggaga ctcgctgacc
    21841 gtcaccttgg agaccgacct cggacaggtg cccctgtcgg tgatgatccg caccttcgac
    21901 ggcgccaacg agacctacaa cccctcccac tcgatgtcgg gctcggcatc gatgtcgatg
    21961 tcctcgtcat gacccccgct gccggatgtc cagccggccg gactactccg gccggcccgg
    22021 gccggcgcgg cgtgctgacc accgccgtcg tcgctggtct cgggctgggc ggatgctcgc
    22081 tcacggcggg atccccgtcg gggacagcgg aatcggcgga cctggtcgcc agcggcgcga
    22141 cgacggcggg agccgcaccg gcggaggctg tgcagccctt ccgggcggcc cggcaacccg
    22201 ggatccgcgg tcgcgtgcag cccttcgccg ctttcgtcgc gttcgacctg accccaggtt
    22261 cgtcggcgcc ggacgtccgg cgcatcctgc gcatctggtc cgacgacatc gagcgcctga
    22321 tgtccgggcg cccgcccctg accgaccccg aaccggaact ggcgcggcat accgctggcc
    22381 tgacggtgac cgtcggcgtc ggaccccgga tcgtcgaact tgtccgcggc gcggacgcgg
    22441 tgccggcctg gctcggccca ctcccggcat acccccagat cgacaaactc gaagaccggt
    22501 ggagtggcgg cgacctgctg ctgcagatct gcgccgactc ccccaccacc ctcgcacacg
    22561 cgcagcggcg actcaccttg ggcgtgcggt cgatggccga aacccgatgg gtgcaaaggg
    22621 gattccgcga gccgctcgac accggcgagc acctcatcgg aatgcgcaac ctcttcggcc
    22681 aggtcgacgg cacgatccaa ccggccgacg acgagccggt gttcatcggc gcgggcgggc
    22741 cggactggct gcgcgacggt tcgagcatcg tcatccgccg catccggatg gacctggaca
    22801 aatgggagca ggtggaccgg ctcgggcggg agaacgccat gggccgccgg ctcaccgacg
    22861 gcgcgccgct gaccggcggt gccgagatcg acatccccga cgtcgacgcc aaggacgcgc
    22921 tcgggttccc cgtcatcgac cccggcgcgc acgtccggcg cgcccgcgcg accacgaaca
    22981 ccgaacgctt cctgcgccgg ccctattcgt atgacgacgg gaccggcgag caggtcgacg
    23041 cgggtctgat cttcgttgcc tactgcgccg acccgctcgc ccaattcgat ccggtgcaga
    23101 agcggctggc cgccaaagac ctgatgaaca tctggaccac cccgatcggc tccgccgtct
    23161 tcgccatcct gcccggaccc ggcgacggag agagcctggg gcaggcgctc ctggcctgat
    23221 ccgacttcgt cagatcgacg tgggctccgg caacaaggcc ccggccagaa tctcgagcag
    23281 ttcgaggacg atgcgctcgg gggcgctcgg gtgccggcga tgcacggcgt acaccggcac
    23341 cgggctgacc ggtaagcgtc ggacggtgtc gatcacgata tctgcatcgc ccgccgacgc
    23401 gatatctgcc caagctgacc gacttcccgg gtcacgacaa gatccaattc gcgcgcccca
    23461 tcacgacgtg cggctttgtc gtccagacca agggctctta cctcgggggt ttgggcggca
    23521 tcgaatcccg aagctcatcg gtgctctaca tcacgacgac gcgcctggcc accaagtccc
    23581 ccaccggcgg tcagctggtc gccaagcagg agaacatttc cgtcaccctg atcggcgctt
    23641 gccaataggt cagctcgcct cggcactgca ggcagccgag gcgtcgatgc cgcggcatac
    23701 gcacaacgct gcgggcatag gcaaggcaaa gtgctcgtcc gcaacggaaa tgttccgttg
    23761 cggacgagca cttcgtggca tcgggcgggc acgttaggcc cgcggcttct cgcgcatctc
    23821 gtaggtggcg atcaggtcac cgagctgcag gtcgttgaag gacccgaggt tgataccgca
    23881 ctcgtagccc tcgcggacct cggtgacgtc gtccttgaag cggcgcaggc cggcgacttc
    23941 gacgttctcg gcgatgacga caccctgacg cgtgatccgc gccttggcgc cgcgcttgat
    24001 ctcgccgctg cgcacgatcg aaccggcgat attgccgaac ttggacgagc ggaagatctc
    24061 gcggatctcg gcggtgccga gttcgacctc ctcgaactcc ggcttgagca tgcccttcag
    24121 ggccgcctcg acctcttcga tggcctggta gatcaccgag tagtagcgga tctcgacgcc
    24181 ctcacgctcg gcatactccg cgttctgccc ttcggcgcgc acgttgaaac cgatgatgat
    24241 cgcgttggag gccatcgcga ggttgatgtt gttcatcgtg atggcaccga caccgcggtc
    24301 gatgatccgc aggtcgacct cgtcgcccac gtcgatctgg agcagggcgt cctcgagcgc
    24361 ttcgaccgaa cccgacacgt cacccttgag gatgaggttg agggtgtcga ccttgccggc
    24421 cgcgagggcg gcgttgaggt cttcgagcga gatgcgcttg cgggccttgg ccagcgacgc
    24481 ggcccggtcg gccgcctccc gccgctcggc gatctgccgg gcggtgcggt cgtcgggcgc
    24541 gacgacgaag gtgtcgccgg cgcgcgggac cgaggagaga ccgagcacct gcaccggacg
    24601 ggacggcgtc gcctcgtcca gggtgttgcc gtgctcgtcg agcatggcac gcacacggcc
    24661 gtggctggag ccggtgacga tcgcatcacc gacgtgcagc gtgccggact ggatgaggac
    24721 ggtcgcggtc gcaccgcggc cccggtcgag attggcctcg atcgccacgc cgcgggcgtc
    24781 cttgtcgggg ttggcgcgca ggtcgagcgc ggcatccgcc gtgagcagca cggcctcgag
    24841 caggtcgtcg atgtgcagca tctgcttggc ggagacgtcg acgaacatcg tgtcgccgcc
    24901 gtactcctcg gcgatgaggt tgtactcggt cagctgctgg cgcaccttgg ccgggttggc
    24961 gccgtcgacg tccatcttgt tgaccgccac cacgatcggc acatccgcgg cctgggcgtg
    25021 gttgagcgcc tcaatggtct gcggcatgac gccgtcgtcg gcggcgacca cgaggatcgc
    25081 gatgtcggtg accttggcgc cacgggcacg catggccgtg aaggcctcgt gaccgggagt
    25141 gtcgatgaag gtgatcgccc ggtcgacgcc ctcgtgttcc ttgtggatct ggtaggcgcc
    25201 gatgtgctgg gtgatgccgc cgtgctcggc cgcgacgacg ttcgcgttgc ggatggcatc
    25261 gagcagcttg gtcttgccgt ggtcgacgtg acccatgacg gtcacgaccg gcggccgcgg
    25321 ttgcaggtcc tcctcgtcct cggagtcgat gccgatctcg aggtcgatgt caaaggactc
    25381 gaagagctcg cgctcctcct cctccgggct gaccatgcga atctcgtagc cgagttcggc
    25441 gccgagcaac gcgaacgtgt cctcatcgag cgactgggtg gccgtggcca tctcaccgag
    25501 gtggaagagc acggtcacca gcgacgccgg gttggcgtcg atgcgctcgg ccagatcggt
    25561 caggctggcg ccctggcgga tctggacgat ccggccgtcg ccgcgcggga tctgcacccc
    25621 gccgatcgtc ggcgcggaca tctgctcgaa ctcttgccgc ttcgcgcgct tggacttgcg
    25681 ctgcttggcg cggccaccgc cgcggccgaa agcaccctgg gtgccgccac ggccaccggg
    25741 accaccgccg ccgggacggc caccgccgcc gccgaagcca gtgccgccgc cggggccacg
    25801 gccggcgaag ccgcccccac cggggcgtcc ggcgccggcg ccgggacgac cggcaccgcc
    25861 acgcgcggga cgctcgccgg gccgcgcgat cgcggaccgg tcgggcatca tcgacgggcg
    25921 cgggccgccg ggacgcggac cacccgggcc ggctgcagcg ccaccgggac gctgcggacg
    25981 ggcctggggc cgcggcatac cctgactcgc ggcgaacgga ttgttgcccg gacgcggcgc
    26041 gtgaccgccg gcgccaccgc caccttcgcg gccctggcgc atgccctggc tggaggagaa
    26101 ggggttgttg ccgggccggg gcgcggccgg acgcgggccg gcggcgccgc cctcgcgtgc
    26161 ggccggagcg gcaggtgccg gcttggccgg ccctggacgc gggccgggcg tggctgccgg
    26221 tcccgcggca gggccaggct cagcggccgg tggcgtggag ggcgccgatg caggcggcgc
    26281 ggccggcgcg ggtgcatcgg ccggcgcctc cggagccttc gctgcagcgg gggcagcctg
    26341 ggcaggagcc gcggccggcg cggccggcgc gggcgcgggc gtcggcgccg cgtcggtttc
    26401 cggctgcgcc gcacggggcg ggcgggccgg tccgggcttc gggccgccgg gcttgggacc
    26461 cgccggagcc gaagcggccg gggcgggcgc cggggccgct gcttcggtgg ggtacttctc
    26521 gtggtaacga cgcaccacgg gggcctcgat ggtcgaactc gctgccttga cgtattcgcc
    26581 catctcgttc aagcgctcca ggaggacctt gctgggtact ccgacctctt tggcgagggc
    26641 gctgacacgg atcttcgaca cttctctcct ctactcggtc cgcatcgagt tgaggcggac
    26701 cagttacagc cggggggaac tcatttctga gtgctcatcg gtggctcatc gcggttgacc
    26761 cgctttcggt ctggttcggt caggtttgct tccggcgcac atgcttcgag gtatgccgcc
    26821 acgagtgtga cctccagcgg cgcgctcatt cggagcgccc gcgggaaggc tcgtcgtcgg
    26881 acggccagcg cgaggcattc ggggtccggg tgcagccagc agccacgccc gggcgtccga
    26941 gcggcaggat cggggaccgc ggccgggtgg ccgtcgtgcc cctcccccgc cacgatgcgc
    27001 aagagagccg atcggctatc cgtcacccgg catccgatgc acgtgcggat cgggcccgca
    27061 ggtgccgtgg acgtcgaagg acgcagtcca gtccgatcgg tcttgcccat tctagcgggt
    27121 cggggcggcg cccggcgcct ccccctgcgt ctggcccggc gttcggccag gagcctggcc
    27181 ctcgccctgc ccgcccgatc cgtggtgcgg gtgggtgccg ccccccgctg ccggactggc
    27241 cacgtcgggc cggatgtcga tgcgccagcc ggtcagcttg gccgcgagcc gggcgttctg
    27301 gccctcccgg ccgatggcca ggctgagctg gtagtccggc acgacgactc gggcgctctg
    27361 cgtcttgggg tcgacgatct cgaccgactg cacccgggcc ggcgacaagg cggcggcgac
    27421 gaactcccgc gggtcctccg accagtcgac gatgtcgatc ttctcgcccc cgagttcggc
    27481 catgacggcc cgcacccgcg cgcccatcgg gccgatgcag gcgcccttgg cattcaagcc
    27541 cggcaccttg gagtggacgg cgatcttggt gcggtgcccc gcctcccggg ccagttgcac
    27601 gatctcgacc gtgccattgg cgacctccgg cacctcgagt gcgaaaagct tgcgcacgag
    27661 gttggggtgg gtgcgcgaga gctcgatctg cgggccacgc agcccccgtt tggccgtcac
    27721 gacataacac cggatgcggt cgccgtgttt gtagctctcg ccgggcacct gctcggcgct
    27781 ggggaggatc ccctccacgg agccgaagct caccgtcaca tgccgcggat tcgggctctg
    27841 ctggatgacg cccgcgacga tgtcaccctc gcggcccttg aactccccga ggatctgatc
    27901 gtcctcgacc tcgcgcagcc gcgacaggat gacgtgccgc gccgtggccg cggccacccg
    27961 gccgaagtcc tgtggcgtgt ggtcgaactc cgggccgagt tcgtagcgcg gccgccgcgg
    28021 gcgcgcctcc tcgccgagtt cggcctcggc gtcgagctcc tcctcgggca gctcgatctc
    28081 ctcctgcgcc cagatggtca cgtgcccact tttgcggtcc aattcggcgc gggcgtggcg
    28141 atagtggccg tcggttcgtt cgtatgccga gagcagcgcc ttctcgagcg ccgggacgag
    28201 cacgtccatg gagatcccac gctctgcctc gagggcgcgc aaggtggccg gatcgatatc
    28261 catttactcg tccttctcct cggtgctcgt gaactcgacg gcgatgctgg cgcgctcgat
    28321 cgcggcatac cccagatcgg tctcggccgg cggctcacca ccggccagca ccaccgactc
    28381 ctcgctcgcc cgcacgatgc ggccggtgac ctccccgtcg gccgtggtcg ccttgagcag
    28441 ccggccgaca ttgcggcgat agtgccgggg ttcgcgcagg gccttgccga cgcccggcga
    28501 actgatctcc aggacgtatg ccgcgccgcc gaacggctcg tcacggtcca gtgcgtcacc
    28561 gatcgcccgc gagtggtcgg cgatctcgtc caggctcagc gccggggtgg gggcatcgct
    28621 ccagccgtcg gggccggcag cgggcacgcg atcgagggtg atccgcacga gtttgcgctt
    28681 gcccgcggcc gagacctcga caccgtcgat cacgagggca gggtcggcca cagcggcggc
    28741 ggcgatctct cgcacgcggt cggcggcact catgaccggc tcctctcgtt aagttgtgac
    28801 aggtcactct atgcgaccca acggaccgcg ccctcctttg gtttccgcgt gacatcatcg
    28861 gcccatgtcg tctccccgac cctccgccga cgcgggccgg ccccgccgcg cggcgcttcg
    28921 cggaggcctt ctggccgggg ccggactggc cctcagcgcg tgcggcatcc ggctcgagga
    28981 cgacgccccg aaggtcccgc tcgtgccgac ccggaccccg attccgggtg aggccgaact
    29041 gctggcgctg ctgcacgccg tgagcgcgct ggccgcgtcc gcccccgctg cgtatgccgc
    29101 ggggctgggt cgccaggccg ccgtcctgga cagcgccctg cgcgagcgcg gcgtgcccca
    29161 gtcgtcgttg agcccgtcac cgagcgcgtc ggggtcgggc gccacgtcgt cggtgagcac
    29221 gaccgtctcg agcaccaccg ccgtctcggg cgccacgtcg tcggcgagca gcaccgtctc
    29281 gggcgcaacg tcgtcggcga gcagcacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29401 nnnnnnnacg tcgtcggcga gcagcacgcg cgcggtggtc ggcctgctgc tggccgcgct
    29461 ccccgtagcc gacgccgccc tccgcccagt gctcggctcg atgacggcct cgcacgtgcg
    29521 cctgagcacc ctcctggacg acgccgtccc ggcgccggcg cggggttcgg tcccgaaggc
    29581 ggccgggctg gccctgctcg ccgccacccg cgaggcggtc tacggcaccg aggtggcggc
    29641 agcgcggttc gcggacgccc agcgcaccaa ggcgatcgcg gcgctcgagc ggctacgcgg
    29701 ctgggccacc accttggagg aggacctggg cgcggacgcc ccaccgatgc cgacggcata
    29761 cgccctgccg ctcgacgcga ccgacaacgc gacggcgaag cgcctcatcg cccacctgac
    29821 ccgcagcctc gtgcagtcgt atgccgccgt cctccccacc gtgtccggtg acgccgaggc
    29881 cactctcgcc gcgaccgggt ggctgagcag cgccgtgacg ctcgacgggt cctggggggc
    29941 gacatgggat cccttccccg gattgtcctg agtgcgcgcg gggaatgccg ggtgtcatcg
    30001 tgggtttgaa tagagcatga tcttcggccg ccagcccaag cccatgccga ccgccgacac
    30061 agctctgccc ggccgcgcgc agcccgtgtt cgccgtgccg acgacgcacg aggtgctcgg
    30121 caccccgctc gcgggcccct ggcccgacgg caccgaggtc atccatgtcg cgatgggctg
    30181 tttctggggc gccgaacgga tcatgtggcg cctgcccgga gtggtggcca cagccgtggg
    30241 ctatcagggt gggttcacgc cgaacccgac ctacgaggag gtctgcaccg gggagacggg
    30301 ccatgcggag gccgtcctcg tggcctatga cccggcgcgg atcaccccat cgcgaatcct
    30361 gaaagccttc tgggagaacc acgatccgac caccccgaac cggcagggca atgacgtcgg
    30421 cacgcagtac cgttcggcca tctactggac gagccaggag cagcaggccg ccgtccgtgc
    30481 cacgcatgcg gccttccagg gtgagttgac caaggccggc gcaagcgaca tcacgaccca
    30541 ggagttgcca gccgagcagg ccggcacctt ctggtatgcg gaggcctacc accagcagta
    30601 cctccacaag aacccgggtg gctattgcaa ccacggtccc aacggactga cgtgtcccgt
    30661 cgggatcgcc gatctgccgg cgcaggtgga cgtcgaaccc cctgccgagc accggtgacc
    30721 gaaaacgaca tacgcggcgt ggccggtctc tgcggaacct gtgcccacgc gctgatccgg
    30781 ccgacgaaca agggcaccgt ctatctgcgg tgcgccttgg cggccaccga cgagcgcttc
    30841 gcccgctatc cccgcctgcc cgtccacgag tgcccggggt tcacccgtcc cggtagcgcc
    30901 tagggcgttt cggtgcgccg gccctgggcc cacgggaaga ccatcagcgc cagccacagg
    30961 gcgcccacga cgaaggcgcc gacgccgagc caggccttcc acagggcgtc gttgtcgacg
    31021 aggctggggc cgaaggccac gcccatgccg tagaggacgg cgacgagtcc gacgatgccg
    31081 cgacgctggt ccttgctcat gcccggtctc ccatccacgg aacgacgacg agggccaggg
    31141 cgcacgcgag cacgagcagc gaccccgcca tggtgtagcc gcgctcgacc cgcggcgaat
    31201 cgacgaattt gggaaggaag gcgaacccga cgccgaacag cgccgcgatc acgccggtga
    31261 cggcgagtcc tgtctcgatg cccatcgatt gcgccccttc tcgtctgcgc cctttctcgt
    31321 cgcggacggt ttcgcccgct gcgccacgag tctcaccggg cagcgatgac gaatcaacgc
    31381 gcgggcacac ggatcgggca gcgtcaatgc gtaatctgat cggccgcata cgtagaaccc
    31441 ccgcaaaccg gcgaccgggc ggggtctcgg cgggcgcggg ggcgacgcct gcgccacaat
    31501 tctccgccgg cgaaagcgcc cgttcgtggc tagagcatgg tgagcgctag agcacggtga
    31561 gcgaggactc cggtgcgcgc tcggtcatca ggtcgcgcac cgcggccggg tcgacgaagg
    31621 ccgccgtcgc ccacacatcg gcccagacga gatccggacc aaccacggtg gtcgaccccg
    31681 aacgcgaaac cacctctccc gtgcggggat cggcgatatg cgatcctcgg gcagccgtgc
    31741 cggacgtcgc cacggcccct tcgacgacct cgaccgtcgc tgcgatccgg ccggcctggt
    31801 gcgggtcctc gatcccgatc gaccagctgg gggcgacgcc ccgcattccg gtgccgaagc
    31861 cgacgaccat gtcgccgccg gcgttgaggc agaacgagat tccggggacc aggcgcaaga
    31921 gcgcggcggc ctgctcgatg gcccacccct tcaccacacc ggtggggtcg tatgccggga
    31981 cctcgcccgg cgccgcgcgc caggcggtga agagcccgcc cgtgcgctcc tcggccccga
    32041 ggcagagctc gagcacctcg gcctgccagg gatggcattc gccggcgagg acggcaccgg
    32101 cctgcagccg gcgcagctcg ctgtctgcac gccacaggct gaagacctcg tcgaccttgc
    32161 gcaaggtggc gaagacattg ccggcggcct ggtcgatgtc cgtcagggac gtgtcgatcg
    32221 ccttgacgtg caacgagatc ggcatgccca tgatgaattc gacgaaggct cggcgctgga
    32281 tcggctgggc aggctgggcg gcgcgggcgg ctcgcgggcc gggccgggtc ggcaggggaa
    32341 gggtggtggt cgtcatgaca ggtgcgcctg gtcgatcgcg gactgcagcg actggatgta
    32401 gccgacggag gtgaccgtcg cgccggagac catgtcgatc gacgagttct gcgcgtcgac
    32461 cgtctcctgg ttgaggatcg gcaccgcgta ggagttgatc tcctggtcct tgtggttgct
    32521 ccacggcacc tgcagcacct cggacttggt gatcttgccg ttctcgacgg tgatctggac
    32581 ctggaccagg ccccattggg tgttgacggc gtcgccggcg taggtctggg tgcccgcatc
    32641 ggagctggac gaatccgacg atgaactgct cgtggagctg ctgctggggg ttgagctctc
    32701 cgtggtcgcg tccgacgagg tgtcgtcggt ggtggcgcta ccggtcggtg actgcgacgt
    32761 agcactgtcc gtggaatccg ttgtgcccga gctgctttcg gtcgtcgtgg gagccgcggc
    32821 cgcgaccgag ctgctggcgg ccgcattgcg ggacgtggga tagctgaaca gcaggacaag
    32881 cgcgctgagc gtgctcatca gccagagggt gatcttgcgc attacgggcc tgactctcag
    32941 tactcgaagt gttcgatgtg gatggctttg cgggcggcgc ccgcgtcctt cacggccgcc
    33001 tcggcggcgg ccatccacgg ccccgagccg cagatgtaga cgtcgtggtc ggcgatatcg
    33061 ggtgcgatct ggagaagggc ctcggcgtcg cccagatggg cggccgactc ggggagccac
    33121 gagtcgcgac cgggtatgcg gtgcccgtcg acgacgacgt agcgcgcgcc gcggtgggcg
    33181 gccagggctt cgatctcctc accgaggacg agttcgtcgc gggagctggc ccggtggacg
    33241 atggtgacat cacccgccga ctgcgggagc gactccagca gggcgcgcat cggcgtgatt
    33301 ccgatgccgg caccgaggag cagcaccttg cggttgccgc ggactcccgg gtgcaagcgg
    33361 ccatacggcc cttcgatcag cgccttggtg ccgacctgga gatcggccag ccgggcgctc
    33421 ccggcaccaa gatgcttgac ggtgatgcgc agggtgtcgt cggtcggggc cgcggagagg
    33481 gagtaggggt tggcccgcgt ccagccgggt ccgtcctgaa agcgccagtt gaagaactga
    33541 ccgccctgcg cccccagccg gttcaggccg cgtccacgca gcgtcacgct ggtgacatcg
    33601 ggggcctcgg cggtcaccgc gacgacgcga aggtccgagc ggcgcgagcg aaagaggggc
    33661 attgcgacgc ggaaggccag cacggcggcg gccaccacgg catacaaggt ccaccagaac
    33721 accgtcgccg cacgcgagtc gaggaactcc tggccggtcc acagctggtg cggcagggcc
    33781 aggcccgcgc cgagatagcc atagaggtgg atcaggtgcc aggactcata gcgcagtctg
    33841 gcgcgggcct tcttgatcga ggtgacgacg accatgacca ggcaggcggt gccggcgatc
    33901 gccaggagca tcccgggata gttgacggtg aagtcgacga tggtgcccca gatcccctcg
    33961 gatgtccccg cggcataccc gagggtgatg agcaggatgt gggcgagcat caggttgaag
    34021 ctggtgaacc cgatcaggcg atgggaacgg accaattcgt cctggcccca agccttttcg
    34081 aaccacggca ctcgggccat caggaagacc tgcacgagca gcagggcgga ggcgatgagc
    34141 ccggtgaggc gtccgaagct gctcaggaat cccccgacgc tgccgacgtc ggccagtccg
    34201 ccgccgtgga tccacagcgc ggtcacgccc aggagcagca gccagaccgc ggcgcccgag
    34261 gtgtcgcgcc accaggtgcg ggtgcggcgc agcggcgcgg ccgccgccgg ccgctccgtc
    34321 ggtggggtgg agccgaccca gcgcacgggc ggatccgtgg gaagggtggt ggtgcgttcg
    34381 gctgtcgcgg tcatgacaag aaatgtcccg gcagaccctg tggcgaacct gtggaccttc
    34441 catggtcccg cccagacttc ctcagcccac gattcttccc tcagtccacg attcttccct
    34501 cagtccacga tctcccctca gcccgcgatc tgccagccca gccggatgat gaacgcgccg
    34561 acgaggagga tgaacaccgc ccgcacgaat ccggagccgc gcgcgaccgc ggtgcgggcg
    34621 ccggcatacc ccccgatgac attgcaggcg gccatgatca gcccggtgcg ccacatgacg
    34681 tggccaccgg gaatgaagac cacaagggcg ccgatattgg tggccagatt gacgatcttg
    34741 gccttcgccg tggccggcag gaaggcataa ccgagcagcc cgacgagcgt gaagacgagg
    34801 aaggatccgg tgccggggcc gagagcgccg tcatacaccc cgatggcgaa gccggtcagc
    34861 atcgcgaggg cggtatgccg agtcccgatg tgccgcaacg ccgtcactga tcccaggtcg
    34921 ggtttgagca gtgtgtaggc accgacgagg acgagcatca cgaggacgat cgggttgaac
    34981 gcggacttcg ggatgtgcac gccgatgagg gctccgccca tcgcgccgac gagggccacc
    35041 gccgccatcg gcacggccgt gcgcaggtcg ggccggaccc ggcggcagta cgtcgctgtg
    35101 ctcgagacgg tcccggcgat ggaggagagc ttgttggtcg cgagcacctg ggccggggtg
    35161 gcgccaggca cgcccaacag gagggcgggg atctggacga gtcccccgcc gccgaccacg
    35221 gcgtcgatcc agccggccac gagcgcggcc agcccgagcc acacctcggg cgggagatcg
    35281 ttcacgccgt ccagccggtg acgacgccga ggatctcgcg cagttcgtgc acggtcgcat
    35341 cgggagtggc gtcgacggcg acgcgttgcc tgggcggcag gtccgagtgc gggatccaga
    35401 tggctcgcat cccgatctgc tgcggccccc agacgtcctc gtagatccgg tcgccgacgt
    35461 agaccgccgc gcccggttcc acgtcgagtt gccggaggat ggccgcgaag gcctccgcgt
    35521 gcggtttgac cacgtgcagt tcggacgaat aggcgtccgc gtcgatcagg tcgaggacgc
    35581 cgtcccgggc gaagagctcc cggtggtagt cgcgggtcca gatcgtgttg gacaggacgc
    35641 ctatcttgat cccgttctct cgcaaccctt cccagagcgg ccgcacctgc gggtcggtga
    35701 tcgtgtgcgg ttcccagaag cgccggtagg ccgcgagcgc cagcaggtgc cggtcgtgct
    35761 ccgggtcgac gcccgcggcc tcgagaatgg catcgaggtg ggcgctcgtg tggtcggcgc
    35821 gtccgcgggc ccacgcgtcg tcctcgaccg cgatgatccg gtcggcgagg tcgtgcgccg
    35881 ccctcaggtc cgcctcgctc acctccggtg agtcgacggg tatgccgtgc acctcgcgcg
    35941 cgaaaacccg ccactgctcg ccgatgtcga cggcgtgcca aggagtcagc gtgccgcccc
    36001 agtcgaagac gacggcctcg accccgggtt gcgaacgctc gtggctcatg gagtcaggat
    36061 cctacgcggt ggttggcgtc gtatcctcgg cagagtgact gcacccgcgc cctcgggccc
    36121 tgtccctgtc aacccgtggc cggccctgtg ggcgctcgtg gtcggtttct tcatgatcct
    36181 cgtcgactcg acgatcgtcg ccgtcggcac cccggccctg atggccgcct tcgacaccga
    36241 tgtcaccgcg gtgctgtggg tcaccagtgc ctacctgctg gcgtatgccg tgccgctgct
    36301 gatcaccggc cggctcggcg accggttggg tcccaagcgg gtctacctcg ccggcttgac
    36361 gctgttcacc ctcgcctcgc tgtggtgcgg cctgagcggg gaactgtccg acagcatcgg
    36421 catgctcgtc gtggcccggg tgttccaggg cctgggggca tccctcatga ccccgcagac
    36481 gatggccgtc atcacccgga cctttccgcc ggagcgccgc ggcagcgcga tggcgttgtg
    36541 gggggcggcg gccggggtgg ccacgctcgt cggcccgatc ctcggaggcg tcctgctcga
    36601 cagcctcggc tgggagtgga tgttcttcat caacgtcccc gtcggcatca tcggctggct
    36661 gctcgcctgg cgcctggtgc cccgtttgga gacgcactcg cacagcttcg actgggtcgg
    36721 cgtcgctctg agcgctgtgg ggctcttctg cggcgtcttc gccatccagg agggccacgc
    36781 ctatgactgg gggcagatca ccggcgtgat ctcggtgccc ctgctgctcg tggtgggcgc
    36841 cgtgctcctc gggctgttcg tctggtggca gagccggatc cggcgcgaac ccctcgtgcc
    36901 gctcggcctg ttccgcgacc gcaatttctc ggtctccaat gtcgccatct cgaccgtggg
    36961 cttcgcgatc accgcgatga tgttcccgtt catgatctat gcccaggcag tgcgcggcct
    37021 ctccccgacc cgtgccgcac tgctcctggc gccgcaggcg gtgatgtcga tcgtccttgc
    37081 tcccatcgcc ggcaagctga ccgaccggat gcaccctcgc ctgctggcgg gcttcgggct
    37141 gctcacgctt gcggctgtca ccttcgccct gtcgcgggtg atgacgccgg gctcgtcgac
    37201 atggtcgatc ctgctcgtcg ccctggtcct cggcgtcgcc aactccttcg tctggggtcc
    37261 gctcgcgacg tccgcgaacc gcaacctgcc gccgcacctg gcgggggccg gtgccggggt
    37321 ctacaacacg acccgccaga tcggcgccgt actgggcagc gctgcgatcg ccgttctcat
    37381 ggaagctcgc atcgccgcgc tgcttcccgg tatgccgggt ggggcctcct caatggccga
    37441 gagtggcgct gtggccatgc cggaagcggt gaaggagccg ttcgcgcagg cgctggcgga
    37501 gtcgatgctg ctgccggcgg cggtggtgct cctcggcttt gcggccgctc tgttcttcga
    37561 gcgcccgcgt caccagcaga cttccggcta aggcgttccg aggctacttc cacacgacgg
    37621 cgacaaagcg gaacgccgag ccgtcgtagc tgccggccat gccgaccacg gtcccggccc
    37681 tgttgacatc ctggccttgg ctcaacatcg tgcccgacgg ctgcggcagg taggtcaccg
    37741 ggctgctcgg tgacgcccaa cggatggcgc gctcctgact gccgtccgtg ccgacgatga
    37801 ccccgccgtc gttgagggct tgggcggtaa gggagttgcg gccaccggtg ggaagagctc
    37861 ggaggcggcc cgaggaccag atgaagggga cgaggcggct cccggtcacg atcgaggtcc
    37921 ccacgacctg gcccgcgttg ttgatgtcgt tgggcgtggt gccgccgccc agggtgccga
    37981 gctgggtttg cgttccggcc gaccaggtcc atgcccggta ctgccccagt gggctcgggt
    38041 ccgcccacaa ccagcgcatc gtgccgacca cgcggccgga gtcgttgatg ccttcggcgc
    38101 tggccccaat cggggccgtg atcgtgcgag cgcttgcttg gccgggcctc cacaggaagg
    38161 ctcggcgggt ctggaagggg tcgtcgccgg ggacgttgta ggtcccgacg attcccgctt
    38221 tggaggtggc gacggccatg ccgaaatcgc cgggcgcggg gatccgcgtc aggcgcccgc
    38281 cttcccacat gaccggcttg aacgtcccgg ccgcggtcat gcagccgccg acgatgcgcc
    38341 cggagttgtc gatatccctg gcctgacact cggcgccgcc cagggcagga agccgggtga
    38401 tccgcccgcc cgtccactgc accgcgtagt agcgcccgtc cgtggccaag gcggtgccgg
    38461 ccacgacgcc ggagtcgttg acggcggcag cggaggcaga ggacccgccc agtcctggca
    38521 gtacggtcgg ggtcaggacg gcggccgccg gagtcgtcgt gtcgggcgcg gccgtccgag
    38581 gtgaaggcgt ccggggtggc accgggtccg acgccccggc cgccggtccg gcgagggcgg
    38641 cactcgtgac catcatgagg ccggcgacca gtgaccgacg catggtgtca actcccgtct
    38701 cgcccgggcc gatcccgggc acgtgcccac gctagggccg gcaccggcgc tcgagcgccg
    38761 cgcccgggtt acggagtcct tacgtccgag accgcaccga cctcacgggc tggtggtggc
    38821 cgagcccgcg acgaaccagg tccacggggt cggcacgccg aacgcggtct gcgcggtgag
    38881 cccggaggtg tcgaacccgg cggccgccac gagtctctcg atgtcccgat ccacatgaca
    38941 gcccgatgcg agccgccccc aggcgggctg catggcccgc tgcgccgcag cgagccacgg
    39001 ccgcggcgag agaccgtgtt cgacgaggcg cagggtgccg cccggccgca gcacccggcg
    39061 cgcgtgcgtc aacgccgcct cgacgtccgg gatggagcac aggctccagg cggcgacgac
    39121 gacgtcaacg ctgttgtcgg gcagcgagat cgcggcggcg tcggtgccga tccgagtcac
    39181 cggccggccg aagtcggcga tggctgcgcc ggcccgttgc caaccctcgt ccgagggatc
    39241 gacggcatac acctgctcga ccgccaccgg gtagtgcggc aggttcggcc ccgatccgaa
    39301 gccgagatcg agcacgatcc cgtgcgcccc cgctgtcagt ccgcgccgcc attcaccgac
    39361 gagccggtcg gagagcacga ggtcgacggc gctggggaag gcgtgcttct cccaccagcc
    39421 ggccgtgtcg ctcatcgtct gtcaggctga agtgacttct cgggtgatct ccgcgatcgc
    39481 gtcggcgacc gggacggtgc gccgctcccc ggagcgccgg tctttgatct cgatggtccc
    39541 gtccgcgagg cccttgccga cgacgacgat cgtcgggatg ccgatcaact ccgcgtcctt
    39601 gaacttcacg cccgggctgg cctgccggcg gtcgtcgaag aggacctcga ggcccccggc
    39661 ctccagcgcg cgggtcagct cctcgccgta ggtcaggatg ctgtcgtcct tcccggcggc
    39721 gacgatgtgc acatcggccg ggctgatatg ccggggccac accaacccga actcgtcgtg
    39781 gttgccctcg gcgatcgccg cgacggcgcg ggagacgccg atgccgtagc tgcccatgat
    39841 caccgtctgc agcttgccga actcgtcgag caccttcagg tccagcgcct cggcatactt
    39901 cgtgccgagc tggaagatgt ggcccatctc gatgcctcgc gccgacagca gcggcgaccc
    39961 gcagctgggg caggcatcac cgggacggac ctcggcggcc tcgatcgtgc cgtcggccga
    40021 aaagtcacgt cccgcaacga gatccaggac gtgacgaccg ggctggtcag cgccggtcac
    40081 ccaggcagtg ccctcgacga cacgcgggtc gagcaggtag cggagaccgc tggttccctt
    40141 ctcccccaac gcacccgggc cgatgtagcc cttggccagc gtcgggtatg ccgcgaagtc
    40201 cttctcctcg aacgccgtca ccgtcgcggg ttcgacctgc gcaccgagcc gcttctcgtc
    40261 gacctcccgg tcgcccggca cgccgatggc gagcggctcg cgggtcccgt cggggtgcac
    40321 gagcatgacg acgacgttct tgagcgtgtc ggccgccgtc cactcccggt cggccttggg
    40381 gaaagctgaa ttcaggtgtg cgacaagggt ttcgatggtc ggcgtgtcgg gggtgtcctc
    40441 gacgtgggaa gccggcagcg cgtcatacgg ctgcgccggc ggcgcggtga cctgcacggc
    40501 ctcgacgttc gcggcatacc cgcagttgtc gcagcgcaca taggtgtcct caccgttctc
    40561 ggcggtggcg aggaactcct ccgacttcga cccgcccatg gcgccggcca tcgcctggac
    40621 gatgacgtag tggaagccga gccggtcgaa gatcttgacg taggcgctgc ggtgcaggtc
    40681 gtagctcttg tcgagcccgg cctcgtcgat gtcgaaggaa taggagtcct tcatgacgaa
    40741 ctcgcgcccg cgcaggatgc ctgcgcgggg ccgggcttcg tcgcggtact tggtctggat
    40801 ctggtagagc gcgagcggca gatccttgta ggaggagaac atgtccttga ccagcagggt
    40861 gaacatctcc tcgtgggtcg ggccgaggag gtagtcgccc tcgcggcggt ccttgagccg
    40921 gaagagattg gcaccgtatt cgctccaccg tccggtcgcc tcgtagggct cgcgcggcag
    40981 cagggcgggg aagagcaact cctgggagcc gatctcgtcc atctcctcgc ggacgatctg
    41041 ctcgacccgc ttgaggacct tcagacccaa gggcaaccag gtgtagatgc cggggctgac
    41101 gcggcgaatg tagccggcgc ggaccagaag ctggtggctc gggacctccg cgtccgccgg
    41161 gttctctcgc agggtgcgaa ggaacaggga cgacatgcgc atggccacgg ggtaactcct
    41221 gagggggttg aggacaacgc ccacaggata cgtggccggg cggctcagcc gagcgccgtt
    41281 ttcagggcgc cggcggtctc ggcgagcacc cgctgcccga gcggggcggc cggcatcgtc
    41341 agcatcggat gcacggcgtc ctcgtggaca cgcacggtga catcgacccc ggcggcgcgc
    41401 atcttctcgg cataggcgat gccttcgtcg cgcagcgggt cgaagccggc gacggcgaca
    41461 taggccggcg gcaacccggc caggtcgtgg gcgagcagcg gcgaggcgcg cacatcgctc
    41521 gggtcaccgt cggggccgag gtagttcgcc tcataccagt ccatgttcgc cttggtgagg
    41581 aagtagccct cgccgaagag ttcgcgcgag cgagtggtcg cgcccagctg ggtgaccggg
    41641 accccgagca gttggtatgc cggcatgggc ccgccctcgc ggtgggtcac ctgggcgacg
    41701 accgcggcca ggttgccgcc ggcgctgtcg ccgccgacgg cgatccgctc cgggtccagg
    41761 ccgagctgct cggcgtgctc atgcgcccat cggaaggccg ccaccgcgtc atccaccgca
    41821 gccgggaagc ggtgctccgg ggcgagccgg tagccgacgt tgaggacggc gacgtgtccc
    41881 agcccgcaca gcagccggca ggtcgggtcg tgggtgtcga catcgccgag cacccatccg
    41941 ccgccgtgga aatagacaag cagcccaagg ggtttcaggc ctggcgggct gcccgcgggt
    42001 gggtgggggc gatagagccg ggccggtatg ccgcccgccg gcccggggat ggtcaggtcc
    42061 gcggccgtcg ccacccgagc cggcttgccg gcgaacatcc acgagtcgag cgcgagctgc
    42121 cgccgcgcct ccggcacggg caactggtcc agctccggct cgccgagctt cgtcatgatc
    42181 gccagcccga cgtgggtgtc cgggtcgagc cgggccccat cgacgacatg cgggtgcagg
    42241 tcgaggcggc ggcggagccg gtccgggacg gcgagcatcg cctcgaaggc gcgtttctgc
    42301 gcacgctcgg cgcgggtcgg ggtgaaggcg ggcggcatac ccctagcttg ccaaggccgg
    42361 ggcggtctcg ggctcggtgg ccccgtcgat gtggaggact tcctcgtcgg cgtgccggcc
    42421 gaaccacaag gtgacgacgc cgacgagcgc ccaggcggcc aggacgagcc aggggcgcaa
    42481 ggtcgaggcg tcggggaagt aggactccag ccgcagcagg gtgccggcgg cgccggggac
    42541 gaagaactgt ccgatctcgc cccagtgcca gggcagcatc tccttgggga tggtcagcga
    42601 ggagagcggg ttgccgatga agagggtgac gatggcgccg ggggcgatgc cggcgcggcc
    42661 gatcaggccg ttcatgccga cgatgaaggc gccggtggcg aagagggcgg cgctgatgat
    42721 cgcccactcc atcacgaagc tgccgacgag gatgccgagc cagggcccga ggaccgcggc
    42781 gagcaggagg ccggcgagca cggcataccc ggtcaccgcg gcgaggcgac ggcggtgtcc
    42841 ggcgacgagc agggagatca ggacgccgcc gagcatgcct cccatggcca gcggcaggcc
    42901 ggcgacgccg aagcccgcgc cacgagggtc gtcggggctg agcggggcga tatcggtgac
    42961 ggtgaccttg ggcggctgct gccctgccgc ggccgcctgg gccgtgatcc cggcggcagc
    43021 ctggttcagc gcctggctga cgacggtgct ggcggcgctc gcggtgagga actcgacccc
    43081 ggagggcgag gcgaccacgg cgccataggc atcgcgctgc ttgatggcgc tctcggcggc
    43141 ggcgcggtcg gcgaccgtgg tgatatcgaa ggcaccggac ttgccgagcc cgacctggat
    43201 ctgggtcagc gcggccggcg ggccggcgac gacgagcggc aggtccttga ccttgctcag
    43261 gtagctcggc cagacgaacg cgagcagcac gaggaggacg acggtggacg ctccgagcac
    43321 agtgcggagc gttgtcggcc aggtcgattg cgcggtttgg gtcgacggcg cggagtgggt
    43381 cgattcggcg gtgggagcgg ggtgggccat gactggcatc tcctggttct cattggagaa
    43441 tgatcgttcg ttttgtacct tgccactccg cgtccagaat tgcaaacaga acgttcgttt
    43501 tttatgatga gggtatgccg aaggtcaccg aagcccgccg cctggccgtc cgcgaccgga
    43561 ttcgcgatgc cgccctgagc gagttcatgg acaaggggct cgccacgacg tcgatggcgg
    43621 acatcgtcag cgcgtcgggg atgtccgccg gggcgatcta cggccacttc accaacaagg
    43681 ccgagctgac cgccgaggtc gcgcggggcg tgatcgccag ccgcgtcgac atcgtcgccg
    43741 actcggcggt ggcggagcag atccccaccc cgagcgccct gctgcgcacc cttttcgaca
    43801 cgatgcctca ggacgtcctc gacagcggaa tcgtcctgca gatctggggc gccgcgggtc
    43861 cgtcgggcga actgcgcccc atcgcgcagc aggccttctc caccatgcgc tcggtcttca
    43921 ccgaatattt ggcggcctgg cttgtctcgc aagggcattc gcggcgcgcg gccctggccc
    43981 gtgcgcgcaa gctggcgccg gccttcatcg gcctggcgca gggatatctc gtgcaccgct
    44041 cagtgctggg ccgggatctg gcggatacct atctcgacag cgtccaactc ctgttagccg
    44101 acacctgaat cgcccccgcc ctgcccgggc ttggggagca ctcttccggt cgtctcgggc
    44161 ggggtcagcg gctgcggctg ccgtatgccg gccgggcgcg gcgcggcgcg ccgtcggggc
    44221 ggggccgacc cgggccacgg cggccccgca tcgggcgcgg ggcgatgatc gactgccata
    44281 cctcgtccgg gaccgggcgc tcgctcggct cgacgccctc ggcggcggcg ccggcgttgt
    44341 cgaggcgctc gggggcgatc gacaaggcga cctcctcgat gatcttctcc atcgccttgc
    44401 gctgatgcgg cagggcgagg gtgacgacgg ccccggtcga gccggcgcgg gcggtgcggc
    44461 cggcgcggtg gacataggtc ttgtggtcgg cgggcgggtc gacctgcacg acctggctga
    44521 cgtcgtcgac gtggatgccg cgggcggcga catcggtggc gacgagcacc tggaggcggc
    44581 cgtccttgaa cgcttcgagg accttgttgc gcatcgactg attgaggccg ccgtggatcg
    44641 ggccggccat gatgccgctt tcgcgcagtt gtcgcgcaac gcgatccgcg cccagctggg
    44701 tgcgcacgaa gacgagcgtc ttgcccggcc ggccgctgat ctgggcggtg agcgtcttct
    44761 tcatcgacgg ctcgatgagc agcacccggt gctccatggt gctcacggcc gcggtcggat
    44821 cgtcggtcga gtgcgtcacc gggtcgacga gatacttctg cgcgagttcg tcgatcccgc
    44881 ggtccagggt cgccgagaag agcagccgct gaccaccctc ggggatcagg tccatgatct
    44941 cggtgacctc gggcaggaag ccgagatcgg ccatgtgatc ggcctcgtcg aggatgacga
    45001 tctcgacgtc gtcgagcagc gcagcaccgc gctcgatcag atcctgcaac cggcccggcg
    45061 tggcgacgat caggtcgact ccccggtcca gcgccgtgag ttgcggcggg tagggcatgc
    45121 cgcccgcgac gaccttctgg ctgacgccga cggcccgcga gatcggctcg agagcgtccg
    45181 aaacctgaag tgccagttca cgagtcggca ccagcaccag cgcccgcggg cggttcgggg
    45241 tggcacgctg accgctcaaa cgcgcaatcg tcggcagccc gaaggccatc gtcttgcccg
    45301 agccggtctg tccgcgaccg agcagatcct tggccgcgag cgcgtcggga atcgtggccg
    45361 cctggatggg gaacggccgg cgaatcccgc ggctcgccag cgccgtcacg atcgcctcgg
    45421 gaagcccgag gtcggcgaac gtcggcccac cgtcctcgtg gtcgaccgac gccggcgctg
    45481 gtggtggcgt ggtgtcgggt atgccgcgcg accgcgcccg ctcctcccgc accgggcggg
    45541 gggtatgccg cgccgcctcc cccgcacgcc ctgccggccg gcgagtgggg cgctcgtcgc
    45601 gttcccacgg ccggactccg aaccgttcgc cgccccggtc gtcgcgggaa gaccggttct
    45661 cccgggaggc ccggtcgtcg cgagagcccc ggtcgtcgcg ggacgggcgg cgctcatcgc
    45721 gaacccggaa gtcaccgcga tcgtcgcggt acgacccgcg ctcgccatcc tcccggaacg
    45781 gacgccggtc gtcacggccc cgggagccat cgcggtcctc accatacggg cgacggtcgc
    45841 gttcctcccg gaacggacgg tcgtcccggt cccgccgata gccccggttc ccccgatcat
    45901 cacggaaggc gcgggcaccc cggtcctccc ggtcgtttcg gcctcgctcg tcccggccgt
    45961 agggacgcga tccgcggtcg tcgcggtacg gtgcccggtc gtttcggccc cgctcgtccc
    46021 ggccgtaggg acgcgacccg cgatcatcac ggcgctcgtc ccggccgtag ggacgcgacc
    46081 cgcggtcatc acggcgctcg tccctgccgt agggacgcga gccgcggtca tcacggcggt
    46141 cgtcgcgaga ggggcggcga tcgcgcggat ctgattgcgc ggcatacgaa tcgtcgcggc
    46201 ggcgcttgcc cttgtcggat gactccggcc gcgcgggctt gccgtcgacc cagcgcggct
    46261 tcttcgcgcg tggcgcgtcg tcggcaccgc gcttgccctt gccggcgtcc ttcttcttgg
    46321 cggcggcctt ctgagcggcg ctccagcggg gtttcttctt gggggtggtc atgagtgcgc
    46381 cgtcgcgagg gctcggacga ggtggggcat ggtgttccgt tctttcaggc cgcgagacgc
    46441 cggatggatg cgccgggcaa acaatccatg ataccggagg tgagcaggct tcccgaccgg
    46501 tcaccgggac gcagcagcgt cacttcagga ccgtcacttc aggaccgtca catcagatcg
    46561 tcacatcagg accgtggcga aggtgccgat ctgcgtcatc ccgatgcgtt cataggtcgt
    46621 tcgggcggcg gtgttgaagt cgttgacata gagcgtcgcc aacggggcga cgtcggcgag
    46681 gacgtgctcg acgacggccg ccatggcgct ggtcccgagg ccgtgaccgc gcagttccgg
    46741 agagacccag acgccctgga tctgagcgca gtccagcgcg accgagccga ggtcggcctt
    46801 gaagatcacc cggccgttct cgacgatgac gtaggtgtgc ccctgatcca gcaggcgctg
    46861 caacagcgac cggtagccgc gtgagctgcc gcgatagggc gggtagccga tctcatgcgt
    46921 gaacatgtgt tcggccgcgg gcagcacgag atcgacctca ccgggccgcg cgcgccgcac
    46981 gcgctcgtct ccgacgacgc ccaatcgcga cggtggggtc cgggtcgcca gatgcggctg
    47041 gttcgaccgg acggcgcgga cattcggcca gccggactcg agcagctccc acatgcccag
    47101 cacctgttcg gcgggcccga ggaaggacgc ggcccgccgg ttccatttgc gcacccggtc
    47161 ggcgatcggc ccgcgggtgt atgccgtcgt ctcgacgggc acgacattgg caccggcgaa
    47221 gcacagcgac tgcagttgcc cgtcctcctc atacccgacc acgctctgcc ccggtccggc
    47281 catcccggcc tcgagcaggc gggcggcgac gaagacattg tcggcagggt tgcgtgcgca
    47341 cagccccaac gcggcatcca ggtcggacgg cgtgaggacc cgcgtcggtg tccgggtgcg
    47401 cagcacgtcc cctagcctgc cctgtccggc aggtctagtc acggattgcg atggtgacga
    47461 ttcagccgac ggtgaccgtc ggggcgcccg acaggtcgtc gacgggctcg cccatctcct
    47521 cggccaggcg catggcctcc tcgatcagcg tctcgacgat cttcgactcg gggacggtct
    47581 tgatgacctc gcccttgacg aagatctgcc ccttgccgtt gcccgaggcg acgcccaggt
    47641 cggcttcgcg cgcttccccc ggcccgttga cgacgcaccc catcacggcg acgcgcaggg
    47701 gcacggtcat accctcgagt ccggcggtga cctcctcggc cagcttgtag acatcgacct
    47761 gggcccgccc acacgacggg cacgagacga tctccagttt gcgcggcttg aggttgaggc
    47821 tctgcaggat ctggatgccg accttgacct cctcgaccgg cggcgccgac agcgagaccc
    47881 ggatggtgtc gccgatgccg cgcgagagca gcgccccgaa ggcggtggcc gacttgatgg
    47941 tgccctggaa cgccgggccg gcctcggtga cgccgaggtg cagcggccag tcgccgcgtt
    48001 cgctgagcat ttcgtaggcg cgcaccatga cgacggggtc gttgtgcttg acggagatct
    48061 tgaagtcgtg gaagtcgtgc tcctcgaaca ggctggcctc ccacacggcc gactcgacga
    48121 gcgcctccgg ggtcgccttg ccgtatttcg cgagcagccg cttgtcgagg gagccggcgt
    48181 tgacgccgat gcgcagcgag accccggcct ccttggcgcg cttggcgatt tcgccgacct
    48241 ggtcatcgaa ctggcggata ttgcccgggt tgacgcgcac ggcggcgcag ccggcgtcga
    48301 tggcggcgaa gacgtacttc ggctggaagt ggatgtcggc gatgacgggg atctggctct
    48361 tgcgcgcgat ggcgggcagc gcgtccgcgt cgtcctggct cgggcaggcc acccgcacga
    48421 tgtcgcaacc ggcggcggtc agttcggcga tctgctgcag ggtcgcgttg acgtcggccg
    48481 tcagggtggt cgtcatcgac tggaccgaga tcggggcgtc gccgccgaca tcgaccttgc
    48541 cgaccttgat cttgcgggtc ggccggcgcg cagccaggac gggcgggggc atcgcgggca
    48601 ggccgagaga gacgctcacg gggcacgatt atccctcacc aggatcggcg gtatgccgtc
    48661 cgcgcccgca ggtcagggaa ccggcaccgg atcggcgccc tgtctcgggc cgcggacgcc
    48721 gtcgccctct tctgcgatgg cgtgaccgac gccgggctct cgagcgtgat cgaccttccc
    48781 gacccgggcg tgctgcgtgc actctgcgac gacgaaacgg gagagtccgc ggttgcgctg
    48841 cgcacgcaca tcctgcacgc ccaggacacg gcccgcagcg ccgaggcgct gatcgcgcag
    48901 ggcatacagc tgatcgggca ggagcgggtc gatgcgctgg aacggctccg cacggagcgg
    48961 atccgccgca gcccgatgcc acacgaccag agggggaaaa gatggggcaa gttactcact
    49021 cgcgcccggg tggcggctca ctagagttcc agggcatggc gcaggggatg caggcgatgc
    49081 ggggagacgc gatcgagacg gctggtgacc gggccggccg gaacgccgga ttgggccggc
    49141 ggatatggga gtcgaccgga gcaggcctgg tgcggcgggg actgctcaag ggctcggcgc
    49201 gggcgctgac gggggtctac ggcctctaca aggtgcaccc ggcggtgtgg cgctggacgg
    49261 cgcggaacta ccacccgcag atggagaagc tcgcgcgact gcatgcctgg atgacctgcc
    49321 aattcgcgta tctggacgtc ccggcataca aggaacattt gcggcacaac gattttcagt
    49381 tcaagtggtt cgacctgacg agctacccgc cgaccgacaa ggacggctat gtcaagaagt
    49441 tcaccgagga ggcgcgctgc tgggagggcc acctcgaccg cgtcggcacc gtcgtcgacg
    49501 agtcctccgg ctcctccggc acgcctttca actgggtgcg gggcaagcgg gaactcgaca
    49561 ccatccaccg caatgtcgcc ggcttcacca cgcaggtcta cccgcggcga cggctgttcg
    49621 tcatcaacgc gtactcgatg ggcgcctggg cgaccggcac caacaccggc atcgccatgg
    49681 cgaagatcgc gatggtcaag aacaccgggc ccgatctgga caagatcgtc gacaccatca
    49741 cccacttcgg gccgacctac aactatctcg tcacggcata cccgccgttc gtgaaggacc
    49801 tgcgcgacaa actcgactcc gtcggattcg actgggacag ctacaagctc tacgggatgg
    49861 tcggcggcga aggcatgacc gaggccctgc gcgactactg cgaggaacgc ttcatccggg
    49921 tgctgtccgg ctacggcgcg tccgacctga cgatcggcat cggcggcgaa tcgcggctga
    49981 cggtgtggct gcgcgatcag atcgtcaggg acgaggcgtt caaggaagcc cttctgggag
    50041 aaggggaatc gcggatcccg atgatcttcc agtacaaccc cctcgagacc tatctcgaga
    50101 ccaacgacaa ggacgaactg ctctgcacgc tcaacagcac gaccgtcctg tcgccgaagc
    50161 tgcgatacaa catcggtgac gaggccaagc tgttgacctt cccggggctg gaggccctca
    50221 tcgccaagcg ccccgacctg gaatccggct tccgggaggc ccagagcatc gaccggatga
    50281 agctgcccat ccttcttctg tatggacgcg ccgactccac cgtgagcttc atgggcgcca
    50341 acatctatcc gcaggacgtc gaatacggtc tctacgaagg caatccgcgt gccaacgaga
    50401 tcgaggcgtt catgctgacg ctggaggaga cgacggatct cgatgcgcgc ccggtcgtca
    50461 atatcgccac gcgcgaaggg gtgcatctcg acgacggcag caaagccgaa ctcgggaagg
    50521 cctgcgagac gggcatcgtc gaacacctga tgaaggtctc gcgcgacttc cggcagtcgg
    50581 tcgaggagga tccgtcttcg acgcagatcg ccgtacgcgt ccacgacttc gagaccggac
    50641 cgttcgaggg cggtcagagc aagatcaaga aggtctacat ccgcaaggac cagccggcgg
    50701 acccggcgca ggcggaggcg gcgtcatgag gaccaccgac ttctcgaagg cgccgcggcg
    50761 cgcccaggcg gaggcgatgt tcatcgggtc cacgcgctac aactccctca aggagtgggc
    50821 catcctctcg atgcgctggc gcacgttcgt caagcggatg aaggcgatgc ccggctattg
    50881 ctggcacaag gtgtattggg agccgcctta cacgctcggg acgatcgcct acttcgacac
    50941 catggaagac ctgctgacga tcgcccgctc ccccgagcac cggcagctga tgcgatgggt
    51001 tgcggacggc acggacgacc aggcgaccgc cgggttcatc cgcctctatc gcgccgagcc
    51061 ccacggctac accaacggca tctggcgcgc cgaggacacg agcatggcgc acatcgagga
    51121 gtacaccccg ctgccgggcg acaccggtcc caaacccgtc gacgacccgg ccagcgagat
    51181 ccgctgacca tcgcgagcat gacgcagatc gcgatcgaac gggtcgtcac cgaggcgcag
    51241 ttccaggagt tcgccgacct gccggtgcgc ctgcacggcc acggtcggca cgtgccgctg
    51301 ctccagccgg tgctgcacgc ctggtggacg ggccacttcc cgcaagcgga gcacgggccc
    51361 gtcggattct tcctcgcccg ccgcgaaggg gctgctgtcg gccggatctg cttgcaccac
    51421 aaccaccttt tcgacgacaa gatcggtgcc gaggcgcagt tgttcggcct gtgcgagttc
    51481 gccgacgccg aggtgttggc cgccctgctg ggcaccgtcg aacgggaggc ccgcgcggcg
    51541 gcccgcacct gcctggtcgg gcccgtcgcc ctgctgccca accagaccgg cggcgtgatc
    51601 acctccggct tcggcgagcg cgggttcatg gactcgccgt ggaatcccga ctactacccc
    51661 accgcatacg aatccctcgg gtgcaccagg atcttcgcgg gcgcgacgtg gatctgcgag
    51721 aacttcgaca acctggaccc ggctaccacg ttccgcttcg acgacgcccg gctggcggcg
    51781 gaggggctga acgtgcggcg cgcctcccgc tggcggctcg gtcgcgacct ggagtcgatg
    51841 cgggcggtgc tcaacaccgc cttcgccgtg ctgccctact acacccagat ctccgcctcc
    51901 gacctcgcgg cccagaccga cgggctcgaa gtcctcctcg acgaggcgct cttcctccag
    51961 ttgttcaagc acgacgaacc ggtggcattc gtgctcgccg tccccgacat cagcgaattc
    52021 gtcatggccc gcggtggccg cctcgggatg cgcgaccagc ttgccctcgt agccacccgc
    52081 cggcgctatc gcaccgatgc cattctcatc atcaagggca cccacccgga cgcccagggg
    52141 aaggggtacc tgacgctgct ctcccgcgaa ctgctccgcg gtctgcaggc gggcgactac
    52201 cgccgcctgc gcagcacctt cgtcgaggac gccaacgcgg gctcggctgc gcagtacgaa
    52261 cgcatgggcg gccggccgtt gcacgagacg acgttctatc gcaaggagat ccggtgagct
    52321 gggtcgagga ggtcttggcg gcacgcgctg tctggcagcg ggcgccaagc gctcacaaca
    52381 cccagccgtg ggtgctgacc cccgacgccg atcgcctcgt gctgtcgtgg gacccggacc
    52441 gcgaactcgt ggtcgccgat ccgacccgcc gcgacctgtg gctctccctg ggcgcgctcg
    52501 tcgaggcgat cgccatcacc gaggccggcg ccggccggca gctcgacgtg gactggcaga
    52561 tcgatgccca ccaccaccgc gccgccgtgc tgaccgacgg tggaccagga tcggcgtccg
    52621 atgattcctt tgctgggcaa cagttctcga ccgacgagct gctgcgccga cggacggctc
    52681 gggggccgta tgccgcgact cccgtcgccg agtccactgt ggacgacatc gcggtcgccg
    52741 cccgtctcgc ctccaccggg ctcggcctcg cgttggtccc ggcaccactg gtcgaggtgc
    52801 tcttgcccgc gtcggatcgg tggagctact cctcgcccgc ccaggtggcc gagctgcggg
    52861 gctggctacg gctggaccgg aaccacccgg cacaccgccg ggacgggctc agctacgagg
    52921 ccctcgcgat gaccgccctc caggcccgtg cgctccgaac agccctctcc cccaagggct
    52981 gggccatcct gcgccgcctc ggcggcccgg cgttgctcgc agctgcggga ggcatcgacg
    53041 gtctcgggac ggtggtggcg ctgcatacga cccgggacgc agcggccgac cccgtgcacg
    53101 tcgcggagtc ggggcgcgcg ctgttgcgcg tctggctcgc cgccggccgc cacgggctgt
    53161 cagcccaccc gctcagccaa ctcatcgatg ctccggccac ggcggagaaa ctacatgcag
    53221 ccctcccggg ccgagatcgc ttcgcgctca acgttttccg gataggagtc ccagatcggg
    53281 tgctgccgcc ttccagccgg ctgacgaggt tcaatcaggc gacgagctga gcgctgtgtg
    53341 cgacctggtc aggagggtct cggcgggttg gccggccgtg cacccgctcc tacgctcgat
    53401 agagcacgcg catctctggg cggagggcgg cgacgggcac gatgtccttg ccgaacggga
    53461 cgcaggtgac gggcagcatc ttcaggttgc ccagggcgag cgggatgccg acgatggtca
    53521 gcgcgcaggc gatggcggtg gcgatgtggc ccagagccag ccaccagccg gccaggacga
    53581 accacaacac attcatcagc gccgacccgg cgccggcgtg cggtttggcg acgatgacat
    53641 tgccgaaggg ccacagcgcg aagatcgcca tgcggaaagc ggcgatcccg aaggggatcg
    53701 tcacgacgag caggcacatg atcacgccgg cggcgagata gccgagcgcg agccagaagc
    53761 cggcgaagac gagctagatc aggttgagca gcgtcttcac gcgatcaccc gcgcagccgg
    53821 acggggttga cgatgtcggc atacatcagc agcaccgaca tgacgatgag gagcgaggcg
    53881 acggcatacg ccagcggcag cgccttggtg acgtcgaccg ggcccgggtc agccttgcgg
    53941 gccagcttga aggcccggcg cttgaggccc tcccacaacg cgccggcgac atgcccgccg
    54001 tcgagcggca gcaatgggat gaggttgaag acgaagagtg cgaggttgag ggagccgagc
    54061 agcgcgacga gggtgatgac cttgtcggac gtgctgccga agtcgtccgc ggaggcgacc
    54121 tcgccggcga cgcgcccgac accgacgacc gagatcgggc cgttgatatc gcgttcgccc
    54181 tcgccgaagg cggccttggc gaccccgacc atcttctgcg gaatgttgag gatgacaccg
    54241 gccgtctgcg agatctgttc cccgatgatg cccggaacgg cgctgaccgg ctgcttgacg
    54301 agcacaggcg tgccggccgc gccgaggaag ccggccgtct cggtcttgta ggtgccgtcg
    54361 gtgttggtct tgggtatgcc gtacttgtcg tagaccggca gggtggtcgc catcggcgtg
    54421 gccgtgaccg tgcgacgctg accgtcgcgc tcgacgacca tggtcagcgg cttgtcggcg
    54481 ctgcctcgga tcagcgcgcg cgcgtcgtcc caggtctgca gtgcgacacc attcatcgag
    54541 acgaccgtgt cgccgggctt gagcccggcc gcattggcgg gggcgatcgg catctccgcc
    54601 gtgcactcgg tcttgctcga ctgggcagcc tgggtggtgt cgacgcactg cgacaccgag
    54661 gacagcttgg cgctcgtgac ggagatgccg tggagtgtgg cgaggccggt gaggacgatc
    54721 gccgcgataa ggaggttcat cagcggtccg cccagcatga cggtgatctt cttgggcgtg
    54781 gagagcttgt agaagacgcg gtcctcgtcg ccgggccgga tctcctccat gctctggccg
    54841 cgtgcctcgt cgcgcagctg ggtgaagcgg ccggtgctgg agacgcgctc gccctcgccg
    54901 ggccggggcg ggaacatgcc gatcatgcgg atatagccgc cgagcgggat ggctttgatg
    54961 ccgtattcgg tctcgccgcg cttggtcgac cagaccttgg gcccgaaacc gaccatgtat
    55021 tgcggcacgc gcaccccgaa gcgtttggcc gggacgaggt gcccgatctc gtggagcgcg
    55081 atggatgcgc ccacgccgag cacgacgatg agcaccccga tcagataaag cattgtccgt
    55141 cccttgaatc acgaaaccgg ttggatcacg acagcggttg gatcacgaca gcggtcgcgc
    55201 acacgttcaa cgccacaccc cggcgatttg atcccgtcag ggcccgggcc gcagcgtggc
    55261 gaagccaggc actgccacct gcgtcaagac taaacggtgc ctgcgacacc gcggccacgt
    55321 gccagactgc cgcatgtgcc ggaccacacg accctgcccg agcgtgccct gccccgggcc
    55381 tcccctgccc catcgtcccc tgcccgagag gcttctggcc gcgctggcca ccgcgacgcc
    55441 ggagctggtg gtcgccgccg acgaacccgg agccggccac tgggccggtg cgccgtatgc
    55501 cgtccgcgac ggcgctcgca tcgtgctggc ctaccgcgtg cgcaagccgg agggccacgg
    55561 acgcgggctg gcgaacatca tcgccgtctc ggacgacggc ctgaccttcc gtgaggtggc
    55621 ccgcgtcgag agcggcacct tcggggcggc ctccctcgaa cggccggcgc tggcccgtct
    55681 cgccgacggc ggctggcgtc tctatgtcag ctgctcgacc cccggctcga aacactggtg
    55741 ggtggaggcg gtggacgctc ccacgctcga ggagcttccg gtcggcaccc gcgcggtcgt
    55801 gctgcccggc gacgacaccg aagcgtggaa ggacgtcgtc gtcgaccgtg ctccggacgg
    55861 ccagtggcgg atctgggcct gccggcatcc gctcgacggc ggacccgacg cagccgaccg
    55921 gatgagcacg tggttcggca cgagcaccga cggtctcgcg tggctcatgc acggaccggc
    55981 catgacgccg accgaggggg cgtgggacca gcgcggtgcc cggctcacgg cagtgctgcc
    56041 ggccggcacc gaggcagggt tgtcggaggc ctcgggcatc gtggcgctct atgacggaag
    56101 agcgagcgcc gaggagaact tccgcgagcg caccggcgtc gcgctcggca taccggaccg
    56161 tctggtggcg gcgggcgggc cggtgcccgc ggcatccggc cgggccttgc ggtatgtcag
    56221 cctggtccag acaccggccg gcctgcggac ctacttcgag gtcaccaccg acgacggctc
    56281 ccacgccctg gtcaccacgc tggttccctg acctcagtcc agctggatga gggcggtggc
    56341 gtgggcccgg gcccaggcgt cggcggcgag cacgtgctcg acgctctcga ccggtgaccc
    56401 gtcgtgggct tcgacgacac gggcgaccgt gtcgacgatg tcgaggaagc cgatcgtgcc
    56461 gtcgtggaag gcgtccacgc acacctcgtt ggccgcgttg tagacggccg gataggtgcc
    56521 gccggcggtc ccgacctgcc gggccagccg gaccgccggg aatgcctcgt cgtcgagggg
    56581 gtggaagtcc caggtctgcg cgcgggtcca gtcgcagggc tcgtcgatgt ccgccagccg
    56641 ctccggccag gagagcccga gcgcgatcgg cacgagcatc cgcggcggcc ccatctgagc
    56701 gatggtcgag ccgtcgtgga actcgaccat cgagtggatc cactgctgcg ggtggacgac
    56761 gacgtcgatg cgttcgaagg ggatgtcgaa gagcagatgc gcctcgatga cttcgaggcc
    56821 cttgttgacc agggtcgcgg agttggtcgt gatcacgcgg cccatcgcgt aattggggtg
    56881 ggccagcgcc tgctccgggc tgacctcgcg cagttcgtcg cggctgcggc cgcggaacgg
    56941 tcccccgctc gcggtgacga cgagccggcg cacctccgcg tggcggccgc cgcgcaacga
    57001 ctgggcgatg gccgagtgct ccgagtcgac cggcacgagc tggccgggcg cggcggcggc
    57061 cttgaccagg gggccgccga tgatgaggga ttccttgttg gccagggcga gggtggaccc
    57121 ggactccagc gccgcgaggg tggggcgcag gccgatcgac ccggtgatcc cgttgaggac
    57181 gacgtcggcg ccggacgccg cgacgcgggt cgccgcgtcc gctcccgtga tgacctccgg
    57241 acggtatgcc gcacggtcac ctgctgcgct cacgcgcgcg gcgatcgccg cgcggatggc
    57301 gtcggggtcg ccggttgccg cagcgacgag cgccggccgg aacgtcgcag cctgctcggc
    57361 gagcaggccg acattgcctc cggcactgag ggcggcgacg gtgaagcgtt cgcggttgcg
    57421 ggcgatcacg tccagggcct gggtgccgat ggagccggtc gagccgagca gggcgacgga
    57481 gcgggtggtg gtcacgctcg ccattctccc cgtctctcga cattctcacc gtctctcggc
    57541 ggtcgaggtc gcctctcagc ggtcgaggac ggtcacgtgc agctggccgg cgtccgcggc
    57601 gacgtccgcg agcacgccga cgatgccgtc gtaagccgcc gggtcgccgg ctcgcagcgc
    57661 accggcggcg tcccagacga gcgcccaatc gtccggacgg tccgtggcga cctggcgcag
    57721 gcagtcgtac agcgcgtcga gattgccgcc gaagtagtcg gggaagtcga gaatctcggc
    57781 gaacagccgg agcgactcgg ccttgtccac acctgccggc acgacgctca cggcgcgccc
    57841 cgcggcatac tcctcgcgca ccaccgcgac aacgctggca tccggcccga ggcgacgaac
    57901 ggcgttgacg gacatggtca ttcaccctcc aggatctgcc ggaagctgtt gtagtggtcg
    57961 tcggtgtagt agcggtcgcc cttggctccg ccgatgatgc gtcgggcgcc gcggtcgtcc
    58021 gagccgggcg tctcgaccgt gtactcgcgg tagtagccac gaggttgctg ctgcaggatg
    58081 cgctcccggt tctggaagac cccgtcgtcc tccgaatagg ggtaggggcc gccggcccgg
    58141 atgagggcca gggtgtcgcg gccctgtgcg ggcagctgcg actcggcaat cgtgtccagc
    58201 ccggagaccg gcgtgaccgg ggcggtgacg gggtccttgg gccctggtcc ggtcgtcggg
    58261 gccgtcggga gcttcggcgt cgtcgcctcg ccgccaccgg tcgcgatggc actggtcgtc
    58321 acgccgccgg tcgtcacgcc gctggtggtc gcgccgccgg gctcgccacc ccggcccacc
    58381 gcccaccaca ccaaagctat gcacacggcg acgccgagca gcatgagcaa ctgccggtcg
    58441 gccttcttca tcggcaatcc ttgggtcgag ggggtcgttc gggctgcctt tagtgtgggg
    58501 catgtgacga gcgcgctcac ccgcctggcc gacggcacgg tcaagcagat caacgtcttc
    58561 accggcacgg aggtttggac cgtcccgggc cgggcgcacc gcccactcgt gcaggaggag
    58621 cgggccaagc acccgatccg ggcgtcccag gcccgatcgc tgtgcgcgtt ctgcgagacc
    58681 cggcgcctcg agacgccccc ggagaaggcg cgactcgtcc gcgccggcgg cggctgggag
    58741 gtactccgcg gcataccggc tgaggaggtc gacgcgacgc aggccgagtt ccggctcgtg
    58801 ccgaacctgt tcgagatcgt ctccctcgac tactggcgca tcaaccacgg cttcgtcctg
    58861 ccacccgccg agaccgcccg ccgccgggcc tacctcgcca cccccggcgg acgggcgcac
    58921 ctgcaccggc tcgtcgatgc ccaccgcccg cagctcgccg cgggcgggga cgaggcgatc
    58981 gtcgaaagca gcctgttcgg cgggttccac gacgtcgtca tcgcccgtcg ccacctcgtc
    59041 gaccgggccc ggtatgacga ccagctcgcc tcctccggcc ggctgacccc cgaggagcat
    59101 gagcgctacc tcgcgttcac cttcgaggcg gcccgaaacc tctacgccgc caaccccttc
    59161 gcgcgcgatg tcgtgatctt ccagaactgg ctacgcccgg ccggggcctc cttcgaccac
    59221 ctccacaagc aactggtcac cgtcgacgag ctcggcgcct ctcgggtcgc cgacatcgaa
    59281 cacctgggcc gcaacccgca cctgctggcc gactatcacg gcgtcctcgc gggcaacggc
    59341 ctcatcctcg ccgaaacgga cagcgcccag ctcgtcgcgg gcgtcggcca ccggttcccc
    59401 agcctcgagg tgtggaacct gcggcgcgaa agcgatctgt ggaatatcgc cgacgacgaa
    59461 ctggccgacc tgtccgcgct cgtgcacgcc ggccacgtcg ccatgggggc gcggctgccc
    59521 gccaacgagg aatggcatta ccgcccgccc acgatgcgcg agcacttgcc ggtccgcgtc
    59581 atcctcaagt ggcgcatctc caatccggcc ggcttcgagg gcggctcggg catctacatc
    59641 aacaccatcg acccgtggac agtcgccgag cgcacgcgac acgagctggc ggccaaggca
    59701 caccggctct cgacgcgggt gcgcctcctc ggctgaaatg tccgacatga ctcccgagtc
    59761 gcccccgctg ccgttcacca ccgccgaact ggcggccatc cgcgccctgt ggcccctcga
    59821 tcccggccgc gccttcacca accacggctc ctacggcgcg gtcccccagc ccgtcctcga
    59881 tgccgtcgcc gcccttgcgg cgcggatcgc ccacaacctc aacgcctttc gacgtggccg
    59941 cgatcgcccg gctgcgaccg ttggtcgtct cctggcggga ggaggagggt catcccaccg
    60001 ccgtgaccta tcagggcacc agcaatgtca cgagctggct cgcggcgccg gcggccatcg
    60061 cccttcacga ggagttgggg tgggagcggg tgcgggcgca cggcatacga ctcgcggagc
    60121 agggcggaca gatcgtcgcc gacgcactcg ggaccaagcc catccccggc gacccggtgc
    60181 cgatgcggct ggtcccgttc gagtgcgagg aacggttcgc cgcgatggcg gcgatccgcg
    60241 aggcgcaccc ggtcgaactg gcgatcaccg agtccccggg agaccacttc gtgcgcgtca
    60301 gcgcccacct gtacaacaca cgcgaggact acgtcgccct cgcgcgggcg tgcgccgcct
    60361 atgtcacgca caactgagag accaacaaaa ctgagagccc acacgacgga gagcccacac
    60421 gactgggggc cgctcagaac tgagcacacc atcagaactg agcacaccat cagaaccgag
    60481 cacaccatca gaactgagca caccatcaga actgagcaca ccacagactt gggcggaccc
    60541 gcgcgggagt gggaaggtaa cggcatgggg actttcgacg cgttgcgcgg ccgcggcgag
    60601 gaggcggaga gcatgccagg cacggcgggt ctggcgtcgg acccggccga cgaggtgacc
    60661 gacgccgaac tgcgcgaccg ggctgccgat tacgtgttgc ccggcttcct cgaccgcgag
    60721 gaggcgacgg tcgcgctgcg ggactacttc gaactggccg acgacgaccc ccggccgggg
    60781 caggtcatcg accagctgtg ggcggcgcgg ctggcggaac aggagacgtg gaccgagcgc
    60841 agcgactatc agcgtctgga atccgccttc gaccgcctcg gcagcgaggg ggtgctgacg
    60901 cggatgaact tcacgtgctg ccagaactgc ggcacgaccg agatcgacga cgaacgcacg
    60961 ccggctccgg ctgcggacta tccgtggcgc gagtgggcct acgccttctt ccaccagcag
    61021 gacgccgaac gccttgccga ggggccgacc gtgctgatgc tctccttcag cgcgttccgc
    61081 cccgccgctg acctggaccc cgaactgctg gcggcggccg ccgccggcga cgacggcgcc
    61141 cggcgcaagg ttgcgtcgga gaccgaccgc cgcgtcggcg aactcgttgt gggggccttc
    61201 cgcgacgccg gtctgaaggt cgactggaac ggcgatccgc gcactcggat tgcggtgcgg
    61261 atcgaggatt ggcgcaagcc gctggaggtg tgatcaaggc gggcgatatc gacgggactg
    61321 gttcagcggc cgagcagccc cgcgatcgtg gtgtcgacgg tccgttcggc gagcgtcatc
    61381 agcgagtcgg tgtccttgcc acgcaatggt cctcgcagcg ccagttcggc gaatccgtgc
    61441 accgcggcga aacacggcca ttcggcgccc tctcgatccg ctggtgcgag gctcgcggtc
    61501 gtgacgagcg cgtccagggc ggcctcgagc gcgacgaagg gcgcgggcca tgcggtatcg
    61561 ccgaacgcgc gttgcgcagc accctctccg aagaaggcga cggagaacca gcccggctcg
    61621 gtcagggcga agcggatgta gccgaggccg acggcccgga gcatggcgat cgcagtctcc
    61681 gtggtgtcgg cttcctggtc catggtcccc atcccggcgg ccatccgctg ctggatcgcg
    61741 acccgcacgg cctgaaggag cgctgccctg tcggcgaagt gccggtatgc cgcattggga
    61801 ctcactccgg cccgccgggt ggccgcgcgc accgtcaggg ctgccggacc gccggcccgg
    61861 gtcaggtcca gtcccgcggc gatgagggcg tctcgcaggt cgccgtggtg atagggacgg
    61921 atctccgaca cggtctcgtc atcctcaggg ctcgacgtgt atacgccgtc aacatatccc
    61981 accgatccgc tagagtcgca cggatggagc ccgacgtcgt gatcgtcatg ggagcctccg
    62041 gcacgggcaa aaccaccgtg gccacgggga tttcgacccg gatggggtgg accttcgccg
    62101 agggcgacgc cttccacccg caagcgaatg tcgacaagat gcgcgccgga gtgccgttga
    62161 ccgacaagga tcgcgcgccc tggctgcggc gcatcgggga ttggatcagc gagcaggtcg
    62221 ccgagggcga gagctgtgtc gtgacctgct cggcgttgaa acgggattat cgcgacgtgc
    62281 ttcgagaggg ccgcccgcag gtgcgcttcc tgcacttgca cgatgacatg gaactgatcg
    62341 cagcgcgaat ggcagcgcgc aaggatcact tcatgatgcc gaccctgctg ccgagccagt
    62401 tcgcgacgct tcagatgctc ggcccggacg agccaggcgt cgtggtgacg gtccgcgacg
    62461 atgtgcccac cgtcatccgc aactccatga cagccctcgg gctgacccct cccgaccccg
    62521 caggcgaagg agtccttgca tgagtccggc gggcggaccg ttcgccgtcc cactccggca
    62581 tttgatccgc tgagtcacgc agccgaagca cgacttagca gaggttacgc ggcggcggcg
    62641 cggattccgc tgcgctacaa ggcgattccg acgtacttgg tttcgaggta ctcctcgatg
    62701 ccctcgaatc cgccctcccg gccgaagccg gagtgcttga cgccgccgaa gggggcggcg
    62761 gggttggaga cgatgccctg gttgatcccg accatgccga actccagcgc ttcggcgact
    62821 cggatggcgc gggagaggtc cttggtgaag acgtagccga cgaggccgta ttcggtggcg
    62881 ttggccttgg cgatggcgtc ggcttcggtg gtgaaagtgc agatgggtgc gaccgggccg
    62941 aagatctcct ccgtcatgca gcgcgcgccg gcgggcacat ccgtcagcac ggtcggggca
    63001 tagaagaagc ccttgccggc gaccggtgca cccccggtca gtgcgcgggc gcccttggcc
    63061 acggcgtcct ggacgagctc gtcgacgccg tcgcgggcct tggccgtgat cagcgggccg
    63121 acgtcgatgc ccttgaccgt gcccttgccc acggtgagag cccccatggc ggcggcgaac
    63181 ttcgacgcga actcctcggc gaccgaggtg tgcacgaaga agcggttggc cgccgtgcac
    63241 gcctccccga tattccgcat cttggccagc atggccccct gcaccgccgc gtcgacgtcc
    63301 gcgtcctcga agacgacgaa cggtgcgttc ccgccgagtt ccatcgacag ccgcagcagc
    63361 tgttccgagg actgctcgac gagcttcttg cccaccggcg tcgagccggt gaaggtcagc
    63421 ttgcgcaccc gcgggtcccg gatgagcggc tccatgacag cgcccgaatg gctcgtcgtg
    63481 acgacattga ggaccccctt gggcagcccc agatcgtcga gcagctgcgc cagggcaagc
    63541 atcgacaacg gcgtctcgtg cgccggtttg acgaccatcg tgcaaccagc ggcgagggcg
    63601 gggccgatct tgcgcgtgcc catcgccagc gggaagttcc acggcgtgat catcagcgtc
    63661 ggcccgacgg gctgcttcat cgtgaccagg cgggtcgcac cgttgggggc cacggcatac
    63721 cgcccgtgaa tgcgcaccgc ctcctccgag aaccagcgga agaactcggc gccataggtc
    63781 acctcgccgc gcgcctcggc gagcggcttg cccatctcga gcgtcatgag gagggcgaac
    63841 tcctcggccc gctcggtgag cttctcgtat gccgcccgca acagttcgcc gcggtcgcgc
    63901 gggggcaccc tggcccaatc cgcttgcgcg gcaacggcag cgtcgagcgc cgctagtccg
    63961 tctgccggcg acccgtcggc gacctgggtg agcacacggc ccgttgccgg gtcgtgcacg
    64021 tcgagcgttg cgccgccctc ggccgggcgc cagtcacctc cgatgaagag ggaggtcggg
    64081 acggattcga gcagtgcttt ctgcgacggt gcagccatac ctgcgatcgt atgccgtcgc
    64141 cccccgcgcg cacacccgcc cgcgtggtca gtggctgtga ccgccgatgt ggccctccgg
    64201 gatgtcggcg gcccgaccgg acagcagcgg cagcgcagga tcccacgtgg tgccgtcgcg
    64261 ttcgtccttg cgcttgcgcg cgatggccga aagcgattcg aggacaacac gattcgccag
    64321 ggcagcagtg atgtcggcgt gatcgtaggg cgggctgacc tcgacgacgt cgacgccgca
    64381 gacgggcagt tcgaggcaga tgcgccgaac ggcgtcgagc aactcgcggg ccgagaggcc
    64441 accaggctcg ggcgtgccgg tgcccggcgc gtgcccggga tcgcagacgt cgatgtcgac
    64501 ggagaggaag acgccttcgc actcgtcggt cgcgatggcg aaggcctcgg tgaggcattc
    64561 cccgaggccg cggtggacga tctcggtcat ttcgtaggag cgcatgttct gctgtgccat
    64621 ccagtcgagc gtctccggtg gcggccagta gccgcgcaac ccgatctgca ggaagcggtc
    64681 gccgcgcaac gccccggact cgatgagccg gcgcatcggc tgcccgtgcc cccacaggga
    64741 gccgaactcg atgtcgccgg tgtccgcgtg ggcgtcgaag tggatcatcg acacccgtcc
    64801 ctgcccgatg gcgttcgcgc accccttggc atcggggaag gcgatcgagt ggtcgccgcc
    64861 gaggacgatc ggtatcgccc ctgccgcaac gactttggtc accgccgctt ccagggccgg
    64921 cagcgccgtc tcgatgtctc cggaatacat ctcgacatct cccgcatcca ggacacgcag
    64981 gtcctgcaga ccgtcggtgc gcagcgccaa cgagggacgc gacccgtcgt gcgggaggta
    65041 gtcggtcatc cggatcgcct gcgggccgaa gcgggtgcct ggccggtgcg acgtgccccc
    65101 gtcgaacggt gcgccgagaa tcacgatgtc ggccccggca taagacgcgg gatcgtccag
    65161 atcacaccga tccacgccga ggaaggtgat gtccggaccg aactgcgcgc catagcgacc
    65221 catggcggca tcctacggag gtggcccgcg tcactcagcc cgaccttggc tgatccggct
    65281 gtggcccgag agtaaaattg gtcatggccg gacaccccgg ccgtgggctc catcgaagta
    65341 gtcctgaaac ctgggacgaa aggccagtaa tgaacctcag tgacgtcatc cgcccgaatc
    65401 tcgacaatat cgtcgttgca gcggcagcag tggtggatgc cgcgggactg ccgcactaca
    65461 tgtcgctcga tccgcagggt gtgaagctgc gcctgcgcga gttggcggaa gccatgctca
    65521 tgagcgtcga aaccggcgag ccggtgcatt tcgcatcgca cgcccaatca atcgcccacg
    65581 accgcttcct cgcgggcgtt cacatcgaag aactgctgac caccttcaac gccctggaga
    65641 ccgccctctg ggagaacgtc gtcaaggtgg cccccaccga ggacgtcgtc gccgacctcg
    65701 gactgatcgg ctcgatcatc ggcgccggca aggaccaggt ggccagttcg tacgtcgaga
    65761 tggccactca gcggcatacc cacggcctcg acatgagtca cctccacgag gccctctagt
    65821 cactccgccg gtagtcactc caccggcagg cactccgcct gcgccgcaac agcgtagccc
    65881 ccgacacccg gtatgggtgg cgggggcttc ggcgtggtga cgactacttc tcctcgatgc
    65941 cccaggccga gccgtagccc tcgggctgcg gagcggccag gagttcgagg aagggttcgg
    66001 cctggaaggc ctcgggaccg aggacgccgg aaccggccca ggtgccgttc gcgaggagtt
    66061 ccagcgcgac gatcggattg atcgcggtct gccagacgat gcattgggcg ccgtactcgc
    66121 gcatcgtcac ctcgttgtcg gccacgtgat agaggtaggt cgagcgtggc cggccatcct
    66181 tgcccgtgcc cgtcacgtgg acgccggcgc aggtcttgcc cgtcagctgc gggccgatcg
    66241 tcgcggggtc gggcagcacg gcagccacga tgtcccgggg agcgacctgc acccccttga
    66301 tcgtgatcgg gtcggtcgag tcgagcccaa gtgcgttgag cgccttgagg atcgagatca
    66361 tctgctcgcc gaggccgtac ttgaacgtga cccgtttggc gtcgagccag cgcggcatga
    66421 gcagcacttc ctcgtgctcg acctgcacac actccaccgg cccgatgccc tcggggaagt
    66481 cgaacacctc gggctcgctg aagggcggca gcgtgaagaa gcccgcctcg agcgaaccgc
    66541 ccttctcggc gcgctccttc tcccacacca ccggcgggtt gaggcactcc tcgatgatgg
    66601 tccacatcga gaacccgggc gcgaacgctt cggagccgtc ggggccgaag taggcgaggt
    66661 tggcgccgtc gcgggtgccg agttcgtcga tctcggagaa gaggttgtcc tgggcgtagc
    66721 gcgcgaagac gtcggacagc cccggctcga caccgatccc gacgagcgcc agcttgcccg
    66781 cctgctccca gtcgtgcgcc ttggcgaact gctcgtcgcc gagcttgacg tgcgtcatcg
    66841 cgaacggctt gtccgggtgc cggttggaga gggacatggc catgtccaga tagttcgcgc
    66901 cggccgccaa acacccgtcg aagaccggca tgacgaaccg cgggtcgacc gcgttgagca
    66961 cgtgcgtcgc acctgtcagc cggatcatgg cggcgacatt ctcggcatcg gaggcatcga
    67021 cgccgacggc gtggaatcgt cgctcgccgg ggcggcgctc ttggaccgcc gagacggtgc
    67081 gctgggcacg ctccacgtcg tagtcggcga cgacgaagac atcgaagaag tcccgttcga
    67141 cggcgatctt tgctgcggcg tcaccgacgc ccccggcgcc gatcatcaac acccgcatgg
    67201 gttttcactt ccttgctcgg gctccggttc gcccttgtct ggatcacgtc gacgctctgg
    67261 gggccggccc ccaggggccg ctacttcggc ttgcgcgcgt tcccgatcac ctggccgagc
    67321 accacgagga gcacggcgat gacgaacatc gagaagccga tgacattggc ctgcgcgggg
    67381 atcccgcgca ggtaggagac atagacgaac ttggggaagg tggtctccga accggacacg
    67441 aagttggtga tgatgaagtc gtcgaaggac aaggagaagg cgagcatcgc cgcgccgacg
    67501 atgccgggca gcaccaacgg gaaggtgacc ttccagaagg tcgaacccgg accggcatac
    67561 agatcctgag ccgcctcctc caggcgcggg tcaaggctct gcaagcgggc cttgacggtc
    67621 acgacgacga agctgatgca gaacatgatg tgggcaatga cgatcgtcca aaagcccagt
    67681 cgcagaccga cattggagaa tccttggacg aagatcgtga gcaggctggc gccgaggacg
    67741 atctcgggcg tcgccatcgg gatgaacgtc agcagattgc ccgtgccacg cccgacgaac
    67801 ttgtatcgga ccagcgcgaa cgcgaggagc gtgcccagca cggtcgccgc caaagtggcg
    67861 atggcgccga tctccaggga cttgaccagc gagtcgcaga ccccgggcgc cccgcacgga
    67921 ttctgccagt gcttgagcgt cgggctgccc tcggggttcc acgtgatatt cgacttgcgg
    67981 tagttgttga acgagaagac gaaggtgtag gccaccggca cgaagagata gagcagcacg
    68041 aagaccgcga cgatcagcgc gaagcgattg gcgaaccacg cctggatccg ccgtccggtc
    68101 gaggggcggt atgccgtgcc ttgggtcgcg gcgctcacag cagttcctcc gtcccgaact
    68161 tgcggacata cacgaagacc agggccagga tcagcgccat caacgtgaag gagagcgcgg
    68221 ccgccgtggg gtacccaccg ggcaccttga agaactggct ctcgatgaca ttgccgacca
    68281 tcttggtgct acggtcggag ccgaggagtt cggcgttgac atagtcaccc gccgcgggga
    68341 tgaaggtcag cagcgtgccc gcgaggacgc ccggcatgct catcggcaag gtcacggtcc
    68401 ggaaggtcgt gaagccgttc gcgtacaggt cgccaccggc ctcgatgatg cgcggatcgg
    68461 cccgctcgag cgaggcatag atgggaagca ccatgaaggg caggaagttg taggtcagcc
    68521 ccgcgacgac ggccatccat gtctcggtga tatgcccacc aggcaggacg tggaggaagt
    68581 tcagcgtctt ggcgaccggg ccctcgtcgg cgaggatctg ccgccacgcg atggtgcgca
    68641 ggatgaagct ggtgaagaac ggcgcgatga tgcagatcat catcagaccg cgccagcggc
    68701 cggccttgaa cgccatcgca taggcgagcg gataagccag acagaaggcg aaaaaggttg
    68761 ccaggccggc aaatagcagc gatctgccga agtgcggggc gaactccgtc agcgccgacc
    68821 aatagttctt gaagttcaga tcgcggtagt agtagccggg ataacccggg aacttcgact
    68881 gcaggctgac ggtgaagagc tggaccagcg ggacgacgaa gaagacgatg agccacaaca
    68941 tgccgggcag cagcagcagg tacggcatcc agctgccgcg tttggccgta tccggaccgg
    69001 ccggggcgcc ctgccccgca ccgatgtggg cgagggcgct cacgagacgg tgtcccgcgg
    69061 cataccgacg ggatctccga ggaccggccc cgccgggatc tcggcaccct ccgcggctac
    69121 cgcgaaggcg tgcgccggat cccattcgat ccagaccggg tcgccgggct tgaggtcgat
    69181 cgggtcgacg tcgagattct gctcgtagac cgaccaggtc gcgccactgg cgacctggac
    69241 cagatactgc gtggccaccc cgatgaacga cacgtcgaca acggttgcct tcatgtcgtc
    69301 gccgagggca accttgcggg tgcggtcgat gacgtcgtga ctgtcacgcg ccaggtggac
    69361 cttctcggga cggaccccga agaggatgtc gccgctgtcg actgcagaac gcgcagcggg
    69421 gatcttcacg gtagagccgt gcacgtcggc gacgaagaag tcgccgtccc ggccggtgat
    69481 gcggccatgg ccgaggttgg actggccgac gaagttggcg acgaaggcgg tcttgggcag
    69541 gtcatacatc tgggccggcg ggcccatctg ctcgatgcgt ccgtggttca tgaccgcgac
    69601 cgtgtcggcc atcgtcatgg cctcctcctg gtcgtgggtg acgtggatga aggtcaggcc
    69661 gacctcggtc tggatgcgct tgagttcgac ctgcatctgc cggcgcaact tcaggtcgag
    69721 ggcaccgaga ggttcgtcga ggaggagcac ttcgggccgg ttgaccagcg cccgggccac
    69781 ggcgacccgc tgttgctgac cgccggacaa ctgcgccggc ttgcgcgccg cgaggtgggg
    69841 caactgcacc agttcgagcg cctcgtcggc catcctcgtc gcgtcctttt gaccgcgtcg
    69901 cttgggcccg aacgcgacat tgtcgcgcac cgtcaggtgc gggaagaggg cgtaggactg
    69961 gaagacggtg ttgaccggac gctcatacgg ccgcgacccc gtcaggtcgg tatcgccgat
    70021 gagaatcctg ccgcgcgtgg gctgttcgag cccggcgacc atccgcaggg tggtggtctt
    70081 gccacaaccc gatggcccga ggagtgcgaa gaaggacccg cgcgggacgc tgagggtcag
    70141 atcgtcgacg gcggtgaaat ccccgaactc cttggtgaca ttctcgatcc gcagatctcc
    70201 cgtggcctct cggaagccgg ccatcagttg cccaccacct ttgcccactc ggcgctgtaa
    70261 tcggcgtctt cttgcgccgt cagcgcgcgg aagccttgga tgttgttctt ggtcagatag
    70321 tcctcgctgg ggaagatgaa gggcgactgg gcgagttcct cgtcgatctt ctccatctcc
    70381 tgcttcgcgc ccacgacggg acacacgtag ttgacccacg cggcgacctc ggcggcgacc
    70441 ttggggtcgt agtagtagtt catcagggtc tcggcattgc gccggtgcgt ggaggtgatc
    70501 ggcaccatca tgttgtccga ccacaaggtg ccaccggatt cggggatggt gaactcccag
    70561 ttgtcgttct cggtctcggc ccgcaggatg aacaggtcgc cgctccagac gatcccggcc
    70621 agcgcgttgc cgctcttgag gtcgttcaag taggagttgc ccttgatccg gcggatgtac
    70681 ccgtcggaga cgagctggtt gaacttctcg atggccttgt cgtattcggg cttgccccag
    70741 ttgccggcga tgtcgacacc ctgccactgc atgatcagcc ccagagtgtc gcgcatctcg
    70801 gacagcacga cgatcttgcc cctcagctcg ggggtgaaaa gatcgtcgag agtcttcagc
    70861 tccttgccga cgcgttcctt gtcgtagccg atgccggcga agccggactg ccaggtcaag
    70921 gagtagttgc ggccggggtc gaaggagacc tccttcaacg ggggcaacag gttgccggcg
    70981 ttcggcatct ggatcagctc cagcggctgg accagctggt cgcggatcat ccggttggcc
    71041 atccagtcgg tcaaggtgat gatgtccttg ccgatgtcct gcttggcctc caactgcggc
    71101 cggaccttgc tcacgtagga gtcgttgtcg tcgatgtcct cggtataggt cgccttgatc
    71161 ccggtctcct tgatgaaggc ctccagggtc gggtaggtct tcccctcgtc gtccatgtcg
    71221 aggtatgcgg tccagttggc ccagttgacg accttctccg tggcggagac gtcggtcggc
    71281 agcttgaccg ccgcgacgcc gccggccggt ggcttcggtg gtgcgcaggc ggtcaaggcc
    71341 gcggcgccgg cgcccatagc gctggcggac agcagggacc gccggctcag ggaggaggcc
    71401 cgggcggagc ggatcagggc gcggacagca ggatccgacg ggggtatgcg gcgcggcaca
    71461 gggagccttt cgtgcggtgt tggtgcgtca ggagtcgagg ttcgccatga cgtgcttgat
    71521 gcgggtgtag tcctcgagtc cgtacatcga cagatccttg ccgtaacccg acttcttgta
    71581 gccgccgtgc ggcatctcgg caacgagggg aatgtgggtg ttgatccaca cacagccgaa
    71641 gtccagggcc ttgctcatgc gcatggcgcg cccgaagtcc ttggtccaca ccgacgacgc
    71701 cagaccgtag tcgacgccat tggcgaaggc gatcgcctcg gcttcgtcgg tgaacttctg
    71761 gaccgtgatg acgggtccga agatctcgtt ctggatggcc tcgtcgtcct gacgcagccc
    71821 ggcgaggacg gtgggttcga ggaagtagcc gtcgcccatg gaggagatcc ggttgccgcc
    71881 ggccgcgaca ttggcgtggc ccggcagtcg ctcgatgaaa ccgctgacgt gctcgagctg
    71941 gcgggcgttg ttgaccggcc cgaagagcgc gtccgcgtcg tccggcatac cgaccttggc
    72001 gctggacttg gcgtaatccg caagtgctgc aacgaaatcg tcgtggatgc ccggcgcgac
    72061 cagaacccgc gtggccgccg tgcagtcctg cccggcgttg aaatagccgg cgattgcgat
    72121 gccctcgacc gcggcctcga tgtcggcgtc gtcgaagacg acgaccggcg ccttgccgcc
    72181 caactcgagg tgcacccgct tgacctgttg gctggcgctc tcggcgacct gcataccggc
    72241 ccgcaccgat ccggtgatcg cgaccaggcc ggggatcggg tgctcgacga gtgccttgcc
    72301 cgtctcccgg tcgccggtga tgacgttgaa ggtgccggcc ggcaggaact cgctcgcgat
    72361 ctcggcgagc aacagggtcg tctccggagt ggtgtcactg ggcttgagga caaccgtgtt
    72421 gcccgcggcg agggcggggc cgatcttcca gaccgccatc atcatcgggt agttccacgg
    72481 ggtgacctgc ccgaccacgc cgatcggttc gcgccggatc cagctggtca tgcccttcat
    72541 gtactcaccg gcggccttgc cttcgaggac gcgggcggca ccggcgaaga agcgcaactg
    72601 gtcgagcatc ggcgggatct cttcgctcgc cgtcagggcg tgcggtttgc cggtgttctc
    72661 cgactcgagc gcgatgaact cctccgcccg ggcctcgatg gcgtcggcga acttcaggag
    72721 ggcctgctgg cgctcgctcg gagtcgtctg gccccactcc tcgaaggccg tggccgcgct
    72781 gcggtatgcc gcgtcgacgt cctccgcgga cgagacgggc gcgctggcca cgaccgcgcc
    72841 ggtgctcggg ttgatcacct cgctgcgggc atcactgcgg gcatcgacga aggcgttgcc
    72901 gatgaagttc ttcaggtcgc gcgggctgct cacgagctct ctctccggga gtcgacggat
    72961 cgtcaccatt gacggctgag cgcactctag ttcgtcgggc gcacgggcga acaggcccgc
    73021 agcccgagaa cggcgagatt taacgcagcc gatacagaag acccgcatgc acgacggatt
    73081 ccgtcgtaaa aaggggttga tcggcatcga atcgtgggca atgatgccgg tatgccacgt
    73141 gccgcccggt ccggaccgca gcggctggac gatgtctcca agcgcatcat cgaactgctc
    73201 caggacgacg ggcgcgcgtc ctacgccacc atcgccaagg cggtcggcct ctcggaggcc
    73261 gcggcccgtc agcgagtcca gcgcttgctc gagagcgggg tgatgcagat cgtggcggtc
    73321 accgatccgc tgcatgtggg attcacccgg caggcgatga tcggcatccg ggtcagcggc
    73381 gagctggcgc cggcggccga cgcgctggcc ggcctcgcgc aggtcgctta tgtcgtcacg
    73441 accgccggga gtttcgacct gctctgcgag gtcgtctgcg aggacgacga cgaactgctc
    73501 gacctggtcg gcagggagat ccgcaccctg cccggcgtcg tctccaccga gaccttcgtc
    73561 tatctcaaac tcaacaaaca gcactacaac tggggaaccc gatgaccatc tatcccgccg
    73621 aagtctggga gcccagctcc gaccggcata caccgaccgg tgccctctat tcgcaggcgg
    73681 cccgtgacca cctctggatg catttcacca ggcactcagt cttcgaggac ccctccgacg
    73741 gcgggctcgg gcatacggtc ccgatcatcg ttcgcggtga gggacatcgg atctgggacg
    73801 accgcggcaa cgaatacatc gacgggctcg ccggcctctt cgtcaccaac gtcggtcacg
    73861 gacgcaccga gatcgccgac gccatggccg cgcaggcgcg cgaactcgcc ttcttcccgc
    73921 tgtggtcgta tgcccacccg cgggccatcg agctggccga gcggctcgcc tcccacgccc
    73981 ccggcgacct caaccgcgtc ttcttcacca ccggcggcgg cgaggccgtc gagacagcgt
    74041 ggaagctggc caagcagtac ttcaagctcg tcggcaaacc gaccaagcac aaggtcatct
    74101 cccgctcgat cgcctatcac ggcaccccgc agggggccct ggcgatcacc ggcatacccg
    74161 cggccaagat tcccttcgag ccgctcgtgc ctggcggctt ccgggtgccc aacaccaata
    74221 tctatcgcgc gccggagcac ctgcgtgacg acgagaaggc gttcgggcgc tgggccgccg
    74281 accgcatcga ggaggcgatc ctcttcgagg ggccggacac ggtcgcggct gtcttcctcg
    74341 agcccgtgca gaactcgggc gggtgcttcc ccccaccggc cggatacttc gagcgggtgc
    74401 gcgagatctg cgatcagtac gacgtgctgc tcgtctccga cgaggtcatc tgcgcattcg
    74461 ggcgcatcgg gtcgatgttc gcgtgcaccg acttcggcta cgtccccgac atcatcacca
    74521 cggccaaggg cgtgacctcc ggctacgcgc cgctcggcgt gatgatcgcc cacgatcgcc
    74581 tgttcgagcc gttccgttcc ggcacaacac ttttcccgca tggctacacg tggggtggac
    74641 accctgtctc gtgcgcggcc gccatggcga acctcgacat cttcgaacgc gaggggctca
    74701 ccgatcacgt caagcggaac gccccggcct tccgggcgac gctggagaag ctgctccacc
    74761 tgcccctggt cggcgacgtc cgcggtgccg gctacttcta cggcatcgag ttggtcaagg
    74821 acaaggcgac ccggcagacc ttcgacgacg gcgaggccga gcggctgctg cgcggctacc
    74881 tgtccaaggc gctcttcgac gccgggatct attgccgcgc agacgatcgc ggcgacccgg
    74941 tcatccagct cgccccgccg ctgacgatcg ggcaggccga gttcgacgac atcgagtccc
    75001 gcctccgctc ggtcctgagc gaagcgccgg atcatctctg acctaccgcc actgacctgt
    75061 cagctgtgac ctggcacctg tgacctggca cctgtgacct ggcaccgatg agcgagctgc
    75121 gcccggcggg ctttcccttg tggtgggagg ggtatgccgc gccctcaccc gccgctgtcc
    75181 cgcctgccga ggcgttgcct tcgcaggcgg acgtggtcat cgtcggaggc ggcttcaccg
    75241 gcttgtggac cgcgtactac ctccttcgcg atgccgccca cctgtccgtg ctcgtgctcg
    75301 aggccgagca cgtcggcttc ggtgcgagcg gccgcaacgg cggttgggtc tctgcgctct
    75361 ttccgctcgg agccgacgcc ctggcggccc ggcacggggc gtcgccgacg cgtgccttgc
    75421 tgagcgagct gcggcatacg gtcgatcagg tcggcgatgt cattgccgca gaagggatct
    75481 ccgaggccgg ttatgccaag ggcggcaccc tctgtctcgc gcgcaacgcc gcgcaggtcg
    75541 cccgggcaaa ggccgacgtc gccgcgggag cggtgtggga caccggcacg ctctggttgt
    75601 cgagcccgga ggccacggaa cgtctcgccg ccacgcagat cctcggcggc acctggaacc
    75661 cgcactgcgc acgcatccag ccgcgggcct tggtcgaggg cctggcggcg gcggtgcgag
    75721 cccgcggcgg gcggatcgcc gaaggggcgc gagtcgcttc ggcggcgccg ggggtggtga
    75781 cgctgagcga tgggacgcgc atccgcgccg gccatgtcat ccgggcgacc gaggcctaca
    75841 cggccgggct tgccggcctg cggccgcggc tcgcgccggt ctattcgctg atggtcgcga
    75901 ccgagccgct gccgcacagc acgtgggctg agatcgggct cgcgcagcgc gaggtcttca
    75961 gcgactaccg gcacgtgatc gtctacggac agcgcacccc cgatgaccgt ctcgcttttg
    76021 gcggacggag tgcgccgtat cactggggtt cggcgattcg ccccggattc gaccaggacg
    76081 caacggtttt cgcactgctg cgcgcggccc tacgagagct cctcccccag ctgggcgatg
    76141 tgacgttcac gcacgcgtgg ggcgggccgc tcggcatacc gcgggactgg caccccagcg
    76201 tcgggtatga cgcgtccgcc cgcgtcgggt gggcgggcgg gtacgtcggc gacggcgtcg
    76261 ccgccaccaa cctcgccggc cgcactctgg ccgatctcgt gacgaaccgg tgtagcccgc
    76321 tcacggccct gccctgggtg aaccaccgct cgccctcctg ggaaccggag cccctgcgct
    76381 ggctcggcgt caacgccggc ctgcgcctgg ccgcccttgc cgacgccgag gaagcccgca
    76441 ccggccgccc cgcccgcttg gcccgcctcc tctccaccct catcggtcac tgacccggcc
    76501 caacccgtca ccacctacac ttcggcgcat ggtcgaccag gcggttctgc gcgcgtattg
    76561 gagtcaccgc caggggttgg acgggtccct ggccggcgcg gacagcgcca cggtgctcga
    76621 gcgcgtcgga tgggcccgct cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    76681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    76741 nnnccaagag ccgccagccg cggctcgctg cactgcggga gcactcccga tgatgagcag
    76801 ccgccatccc gagcccggga tcgaggtgga gtcgaccgaa gagttggatg cccttttggc
    76861 gcagggcatc tcgccgcgcc ggctgcgcct gcaagacctc gacctgacgg gctacaccgg
    76921 gctcgactcc cggaccgacc tcgacgagct ggtcgtcctc ggcggccgcc tctccccctc
    76981 gctcgacgca cacttacgcg cgcacggcgc catcgtcttc cccgacgacc ccgacgccac
    77041 accggtccgc ccctaccgcg cccacctcta tacggcagag gaactgtatg ccgggctcgt
    77101 ggacggctat gcggagacca cggatgcgct cgcctacgac tggtatcaga acagtcagcg
    77161 ccatcccgac gtctttttcg ctctgctgca agcgattcac gacgactcga tctcggatgc
    77221 cctggccgag ttcgtcgacg gtcacgcggt ggtcggggtg atgggcggcc acgcactcca
    77281 gcggggcacg gcggcatacg ctgccgcagc gcgcctcggc ttccaccttg ccgagcacgg
    77341 gttcctcgtc gtcaccggcg gtgggccggg ggcgatggag gcggcgaacc tgggggcggc
    77401 ggcatacggc gccgaccaac tcgacgcggc cctggcgatc gtcgccgagg tgccgagctt
    77461 cgtgcccgac atcggcgcgt gggcggatgc ggggatgcgg gcgaaagccg tgctgcggcc
    77521 ggtgagcgga cagcccgtgc cgcgcagcct cggcataccc acgtggttct acggccacga
    77581 accgccgaac gtcttcgccc aggggatcgc gaagtacttc tccaacgcca tccgcgagga
    77641 ttggctgctc gcgcacgccg cggcgggggt cgtcgtcctg cccggagccg cgggcacggt
    77701 ccaggagatc ttccagttcg cgacgcgcgc ctactatgcc agcaagaccc cgccaccgct
    77761 ggtcatcgtc gacagcgggc actggcgtga cacgctgccc gtctggccgt tgatccaggc
    77821 actcgcggcc ggccgggcga tggccgaccg cgtgacgctc gtcgactcgg tggaggacgc
    77881 cgtggcctac ctgacgtagg cgtcagaacc gctggtcgac gaaggtctgg tcctggatcg
    77941 gcggacggac atacggcatc tgcttcatgt cggggagctt gacgacctcg ggcgccacgt
    78001 cctcgtaact gaactggctc agcaggtggt tgatgcagtt gaggcgcgcg gccttcttgt
    78061 cgtcggacgg cacgacatac cagggggcct gcttgatgtc ggtgtactgg aaggtgttgt
    78121 ccttggccat ggagtacttg acatagagct cgtgctcggc gaggtccatg tcggagagct
    78181 tccagcgctt gaggggttcg gcgttgcggg cctcgaagcg gcgtcgctgc tcgtcgaagg
    78241 agaccgagaa ccagtacttg atgacgacga ttccggagcg cacgagcatc cgctcgaact
    78301 cggggcagct gcgcaggaac tcctcgtgct cgttctcggt gcagaagccc atgacccact
    78361 cgacgttgga gcggttgtac caggagcggt cgaagaggac catctcgccg gcggccggga
    78421 ggtgctcgac gtagcgctgg aagtaccact gggtgcgttc gcgctccgtg ggcttgggga
    78481 gagcgacgac tcgcacggtg cgggggctgg tgcgctcggt gatgcgcttg atgaccccgc
    78541 ccttgccggc gctgccgcgc ccctcgaaga gcacgagcac cttcaggccc tgggactgga
    78601 tccagtactg cagcaacacg agctgctcct ggagacgctc catctccgcc tcgtagaccg
    78661 agttcttgag gcggccgtcg gtcttgtagt cctccggatt gccggtgccg ccggccttgc
    78721 cgacccggtg actgaactca ccggagtcgt ccgcgccttc gggcagatcg acatgcttct
    78781 tgaccaactg gcacctgcct tcccccgccc cagggtgatc cgggtcagga cttggtgaac
    78841 atcacgatgc cgaggcacat ttcgtcgatc gtgccctcac cccaggcgac atagcgttcg
    78901 gggacaccgg ccagggccgg cagccggtca cgcagcgact ggtcgtgcgt gcacttcacg
    78961 gtgagcgtgt ctgacgaggt cagcgtaacc ggctggggga gggcagtcgc gctctggttg
    79021 tcgaagtccc agttggtgat atccagcagg gtgcgttcgt ccggccggcc cgcgttggcg
    79081 acgatgctga ttgcgcgacc gagcaggtgc atgtgtccgg acgtcgcccg gacccgcatg
    79141 gtgccattga ccggccgggt gcacgtctgg gtgggcccga tcggttccga accacacagg
    79201 atgtggagca ggtcggcgac cttcgcctcc gagccgaagc gggcgttcaa gtccttcatt
    79261 gccttctcgc gcgcacacaa cggccccgtt ttgcctgggc ggcacggcaa ttcgacgggg
    79321 gccgggagca gcatcgtctg caggtagtcg taggacccgt catccttcgc cagccgcagc
    79381 cgggcggcgc tgcggtcctg cccctgaccc gcgagcaggt tgtagtggat ctgcatgacg
    79441 atccggctgc ccgtgggcag cttggtgccg atgccgtcgc cggcgactcg ctcgctgacg
    79501 ccaggtgccc aggcgccgag ccagggcgcc tcgtccagat tcaggctggc gccgccgccg
    79561 ccgatccccg tgccgccgaa gcaggtccac ccgtcccccg gctcggaagc gtccttcgcc
    79621 tcggccttcg cgaggtcctg cggctcgacc ttgtagagga tgacgtggtg gacgagttcg
    79681 gcattggccg gcaggaagtg ggtgccggtg atgactgcgt ccgtggcgag ctcggggtcg
    79741 acgaggaagc agcggtagtc gtcggtgccc tcaccggccg gggctttcgg cgtgtatgcc
    79801 gcagccattc gcacttcgac gaagcgttcc cccgggcgca gggcgacctc cttggccatc
    79861 tgagcggtgg cgtcgctcgt gggagctgcg ggggacgccg ctggggtgtg gtggttgtcg
    79921 acgcgctggg gcagcgccgc cgggtcaccg ccgcagccgg cgagaagcag cccggccgcc
    79981 agagcggtga acacggaacg caaacgcatt actccagggt aaggggcact gacccgtgcc
    80041 aggctcggat catgccccgg ttgctgctcg tccaccattc cccgacccgg aacctccagg
    80101 cactcaccga gtccgtcctc gacggcaccc gcgcggacgg attggagggg gtcgaggtcg
    80161 ttgtccgggc ggcgttggag gcgaacgccg aggatgtgct cgacgcggat gcgtatctgt
    80221 tgggcacgag tgccaatttc ggttacatgt ccggtgcgtt gaagcatttc ttcgactcga
    80281 ccttcctcca gatcggcggc gccctggacg actctggcgg agcgggcggc tcaggcgggg
    80341 ccactgccgg ccggccgttc ggcctgtacg tgcacggtcg ctacgacacg acgggcgcgg
    80401 tccgctcggt cctctcgatc gtctccgcgc tcggttggcg gcaggcgtat gacgtgctgg
    80461 aagtcctcgg ggacgtcggc gaagaagcga gggagtcggc atacgagttg ggggccacgc
    80521 tcgctgctca gctctgacac tcctcggcac cagcggtcgg tgcgcctgtg aacgaacctc
    80581 ggggccccgg cgcgacgcct gcgacaccat cctgcagccc gatgtgctgg tcgcgcggcg
    80641 agcggatttc agtcagcgcg atctccccgt cgccccattg ctggccgtcg aagtgctttc
    80701 gcccagcacc cgccgcttcg acctgcatcc caagcgggcc cggtacgaag cggcagggtg
    80761 cgccaacttc tgggtcgtcg accccgacgc cccgtcgatc ctcgcctggg agcttcagga
    80821 cggcgtctac gtcaaggtgg gtcgagccag tggcactgag ctgttcacca ccgacgttcc
    80881 gttcccggtg gagttgcggc cggcggacct gatcgcctga gctcaagcgg ctatcgtcag
    80941 gccgctgccg atcgcggcgc cgaggacgat ggccgcgtct gcccgtggat cggtcaggcg
    81001 ggcgccggcc agggccgccg ccccgtcatg cacgaacacc ggcgccagcc gtcgatccgt
    81061 atgccgcgca acggctttcg cgaaggccga ctggatctcg acgtggccga gtggggcata
    81121 gacgccgagt tggttgtcgt atggctgtcc ggtctcgtcg acgtaggtgg cgagggctag
    81181 cgcgaccggc acgatctccg cgccggg
//
LOCUS       DAOPJG010000002        54129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_624_length_54129_cov_8.085106, whole genome shotgun sequence.
ACCESSION   DAOPJG010000002 DAOPJG010000000
VERSION     DAOPJG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 54129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54129
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_624_length_54129_cov_8.085106"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1079)
                     /locus_tag="PKU75_00435"
     CDS             complement(102..1079)
                     /locus_tag="PKU75_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HPF79370.1"
                     /translation="MRAITIPTPGDPEALVLAEVPDPQPAAGEVRLRVAAAGVNRADV
                     MQRKGFYPPPPRASAYPGLEVSGTIDSLGEGVKGWQVGDEVCALLSGGGYAELVTVPA
                     GQVLPVPPGVSLQHAAALPEVASTVWSNVFLTANLQPEETILVHGGSSGIGTMAIQLA
                     RAVGARVAVTAGSAEKLEVCRDLGAEILVDYKEEDFVERLKAATDGHGADVILDNMGA
                     KYLSRNVDTLAVNGRLVVIGLQGGVQGELNLSTLLTKRAAVIATSLRARPAAEKATIV
                     AAVREHVWPLIEAGEVRPVIQATYPLADAPRAHRDLEASQHIGKLLLLP"
     gene            1132..1593
                     /locus_tag="PKU75_00440"
     CDS             1132..1593
                     /locus_tag="PKU75_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79371.1"
                     /translation="MLSTWSARRSLVASVATLATGLFISLGTAMIANPVIGRSIPPTW
                     WSWPVMAVTALLSGLLFATYVREPGAVPAESQVNRRGAVGGALSFFAVGCPVCNKVAL
                     LLLGSAGAVQWFAPIQPYLGIAGIALLAWALRTRLAGERSCPLSVPGPGPA"
     gene            1682..1840
                     /locus_tag="PKU75_00445"
     CDS             1682..1840
                     /locus_tag="PKU75_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79372.1"
                     /translation="MDKTLISLGAALVAAGISAVPKARAAATLTAAGLVPLAAGIIAN
                     ARKAAQER"
     gene            complement(1877..2461)
                     /locus_tag="PKU75_00450"
     CDS             complement(1877..2461)
                     /locus_tag="PKU75_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF79373.1"
                     /translation="MPSRSAHTTSRVRLTPEERRRQLVGIGLRKFVERPALELSLDEV
                     AAEAGVSRGLLFHYFPSKTAYHRAVVEAAGRRVLRTVRPDAGLTGAPAVRQIVERLIA
                     QIVRRRASYLALVYGQAGPLHDQDDPGATATSLRAGIAELVCRALPGLDPGVTHAWVA
                     YVEDRALHAGADTAREAAHCVAALQALRALEAAG"
     gene            2502..3800
                     /locus_tag="PKU75_00455"
     CDS             2502..3800
                     /locus_tag="PKU75_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF79374.1"
                     /translation="MDFAPSPRAADLTARVRQFLDERIEPVEGELVADIEARRARGED
                     SWAVDPRIEELRAAARAEGLWNLFLPAGHEGPYAAKFGTDGGTGLTNLDYAPVAEQMG
                     RSFMAPLIFNCNAPDTGNMEVLLKYGSQEQKDTWLEPLLDGRIRSTFLMTEPAVASSD
                     ATNMAATAAVDGDEVVLNGRKWWSSGAGHPDCKVGIYMAVTDPNADRHHRHSMVVVPM
                     DHPGVKVERMLSTLGQFDEPLGHGEVSLTDVRLPVDHIIGGPGRAFEIAQGRLGPGRV
                     HHCMRLIGLSEKALELALERSTTRVAFGKPLANLGGNREKLAKARIAINQARLHVLHA
                     AWKLDTFGTAGALSEVAEIKAAVPSMACEVIDLAIQLHGGGGTGPDFPLAAAYAGARS
                     LRLADGPDEVHLGVIGRIMLAPYAARAAQRAGQSSGGVAR"
     gene            3797..4594
                     /locus_tag="PKU75_00460"
     CDS             3797..4594
                     /locus_tag="PKU75_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF79375.1"
                     /translation="MSRETRRVLITGGASGLGLALAKAFLARGDEVLVGDLADERPDG
                     VPAEAAYRKLDVRSQQDWDAALVEVKERWRGLDVLVNNAGVATGGRIDVESFADWQRV
                     IDINLMGVVRGCQTFVPLLKEQGEGRIVNVASLAGLVHAPAMSSYNTAKAGVVALSET
                     LRFELAPFGIRVNVVCPAFFRTNLHTSLQGKDTEVEESAVKLITRAGPDGDVIAAKVM
                     SGIDKDRKIILTDKTGYVASWAKRVARPAYDIAIGSSGIRLAKRGSL"
     gene            4594..5667
                     /locus_tag="PKU75_00465"
     CDS             4594..5667
                     /locus_tag="PKU75_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HPF79376.1"
                     /translation="MAEFVAGAGEVRAEDSFDAAVVAEWLRDNASDASGLDGAPQVQQ
                     FSGGASNLTYLLRWPSRDLIVRRPPAGTKAKGAHDMGREYRIQAALAPVYPLVPPMVG
                     YCADESVIGSEFYVMERLDGLILRRDIPAGIGLDTEAERGALCAALVGALADLHDADL
                     GATGLQELDRGPEYVARQVGGWSERYSRARTEDVGDFEAVMSWLSEHQPADRPHTMIH
                     NDFRFDNLVLDHAEPVRVVGVLDWELATVGDPLMDVGSALAYWAQADDPEAIQAIRLQ
                     PTNAPGMWTRDQVWAAYGARRGLAVSESERRFYELFGTFRLAVIAQQIYYRFFHGQTT
                     NPKAGMMGQFVRILDARCTELLP"
     gene            5664..6350
                     /locus_tag="PKU75_00470"
     CDS             5664..6350
                     /locus_tag="PKU75_00470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF79377.1"
                     /translation="MSRIFLVRHGEASWHSDDYDRLTDRGHAQARAVGVELVARRVVP
                     GVVMSGTLRRHRETAAGLLAGAQWPLTVVEDTRWNELDADDIIRVHDPEHDTMTGALA
                     HFAGRGAPQDFESFFDQAMDRWLGGDGGHVESFTQFVERVNAALDALVAGLAPQATAV
                     VVTSGGVQNAIASQILGGGSDTWRRIFGMFPNTGLMRLTHGERGLRLRSLGELSHLER
                     QPDLLSELIY"
     gene            6362..7114
                     /locus_tag="PKU75_00475"
     CDS             6362..7114
                     /locus_tag="PKU75_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF79378.1"
                     /translation="MTTILITGASSGLGAEMARQFAARGHDLALAARRLDRLAELRAE
                     IQARHPERRVEIRALDVTDDEQVFAVFRGFRDDFGAIGRVIVNAGLGKGAPIGTGRYD
                     ANKATAMTNFVAALAQCEAAMEIFRVQKHGHLVMISSMSAMRGMPKTITTYAATKAGV
                     AALAEGIRNEMAGEPALDIDVSVIYPGYIRSEMNERVKHTPFMVDTETGVKAIVEAVE
                     AKRAHARVPAWPWVPLGTAMRHAPLKVVRKLT"
     gene            7234..7356
                     /locus_tag="PKU75_00480"
     CDS             7234..7356
                     /locus_tag="PKU75_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79379.1"
                     /translation="MFPSERVSTHRVTYEFTAFGKVLDVAQCGPSVTPEPADIE"
     gene            complement(7414..7833)
                     /locus_tag="PKU75_00485"
     CDS             complement(7414..7833)
                     /locus_tag="PKU75_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPF79380.1"
                     /translation="MIVLDTNVISELVRSQPEARVVAWVQALSGDVAITAVTLAELLA
                     GVQRLPKGKRKTALSAAIDEALRPFRGTNSILAFDETAAAAYADVLAGRERAGLPIAT
                     ADAQIAAICRAHHATCATRNTSDFLHTGVDLVDPWSP"
     gene            complement(7864..8097)
                     /locus_tag="PKU75_00490"
     CDS             complement(7864..8097)
                     /locus_tag="PKU75_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPF79381.1"
                     /translation="MASIIVRGLDESVKQQLAEQAREHGRSMEAEVRDILTRAARGPN
                     IGIALMEAAQAVGGVDDLPVPARTDVARAVEFE"
     gene            8276..9862
                     /gene="hutH"
                     /locus_tag="PKU75_00495"
     CDS             8276..9862
                     /gene="hutH"
                     /locus_tag="PKU75_00495"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPF79382.1"
                     /translation="MGSVTSTVILGDEPLTIDQVEAVARSVPPGSDYLGEPIHVEVSE
                     AAWGRVRHTRSVVEGLADDTVAHYGISTGFGALATMHIPVDKRHQLQRSLIRSHAAGA
                     GPEVEREVVRALMLLRIQTLATGRTGIREETLRAYVAMLNAGITPVVHEYGSLGCSGD
                     LAPLAHCALALMGEGTVRDAAGDLRSAAEALAAAGIEPVDLAEKEGLALINGTDGMLG
                     MLCMAIADLRALVTVADIAAAMSVEGLLGTDDVFAADLQALRPQPGQALSAANMRKVL
                     AVSEIRDSHRDPDACTRVQDAYSLRCAPQVAGAVRDTIDHAARVAGWELASAVDNPVV
                     TLDDRVESNGNFHGAPVAYVLDFLAIAAADLASMSERRTDRFLDKARNHGLHAFLADD
                     PGVDSGLMIAQYTQAAMVSEMKRLAAPASVDSIPSSAMQEDHVSMGWGAARKLRRSVD
                     ALTHVLTIELLAAARGIQLRAPLRPSPVSAAVIAALDEVTRTAPGPDRILADDIAEAV
                     YAVADGSLLAAAEAAAGPLN"
     gene            complement(9881..10195)
                     /locus_tag="PKU75_00500"
     CDS             complement(9881..10195)
                     /locus_tag="PKU75_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79383.1"
                     /translation="MVQSFATRQELRSWLEREHSRSSGVWVRVAKAGSGIPSVTFHDL
                     LEEGLCFGWSESTRRAGDDTSYLQRFTPRRTRGTTSERNRRLVVELRTRGLMTPAGEA
                     AL"
     gene            complement(10295..10702)
                     /locus_tag="PKU75_00505"
     CDS             complement(10295..10702)
                     /locus_tag="PKU75_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF79384.1"
                     /translation="MTSTITTKLDLVILDCPDAPALARFYAQILGWQPEGDPDADFVV
                     IAPPEGGISTENPNGRTCLGFQQIPDFVAPTWPGGAHPQQFHLDLSCSGQIEAVEPAV
                     LAAGATVHEHQPAENGNFKVFLDPAGHPFCLIR"
     gene            10884..12539
                     /locus_tag="PKU75_00510"
     CDS             10884..12539
                     /locus_tag="PKU75_00510"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPF79385.1"
                     /translation="MDGARPVRAARGTQLTARQWSTEAPLRMLMNNLDPEVAERPDDL
                     VVYGGTGRAARDWASFDAMVRTLTTLGPDETMLVQSGRPVGVFRTHEWAPRVLIANSN
                     LVPDWANWPEFRRLEHLGLTMYGQMTAGSWIYIGTQGIVQGTYETFAAIAEKRYGGTL
                     AGTLTLTGGCGGMGGAQPLAVTLNEGACLIVDVDPQRLQRRVEHRYLDEVADDLDDAI
                     EKALRAKAERRGHSVGVVGNAAEVFPELLRRGVDIDIVTDQTSAHDPLSYLPIGIDLD
                     QWDEYATKKPEEFTDRARESMARHVEAMVGFLDGGAETFDYGNSIRDEARLGGYDRAF
                     AFPGFVPAYIRPLFCEGKGPFRWAALSGDPQDIAATDKAVLDLFPDNDRLHKWIRGAQ
                     ERIAWQGLPARICWLGYGERDKAGLAFNDLVASGAVSAPIVIGRDHLDCGSVASPYRE
                     TEAMADGSDAIADWPLLNALTATSSGATWVSIHHGGGVGIGRSIHAGQVSLADGTPLA
                     AQKLARVLTNDPGMGVIRHVDAGYERADEVAAERGVRVPMREA"
     assembly_gap    12540..12639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12744..13949
                     /locus_tag="PKU75_00515"
     CDS             12744..13949
                     /locus_tag="PKU75_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HPF79386.1"
                     /translation="MSPSTFQAMWAALSPVGRDAQTGGYRRFAWSPEDHQLREWFAQE
                     ATARGLELTEDRMGNQWAWWGDPDTAGPGLVLGSHLDSVPDGGAYDGPLGVVSALAAV
                     DALRGAGHRPNRPIGVANFVDEEGARFGIALAGSRVITGLLDADRARSLTDGDGITMA
                     EAMRAAGRDPDAIGPDPETLRRVGTYVELHVEQGRALADLDAPVAVGTDIWPHGRWRI
                     DVPGEANHAGTTRLADRRDAVLGLAGVIQGTREAAERRGCLATVGKVAIEPGGVNAIA
                     SHATGWLDARGANPEAVRGVIQDVTAMARAHGGAVHEESWTPVTEFTPDLVSRMREVL
                     GGIPALGTGAGHDAGVLAQYGIETAMLFVRNPTGVSHSPAEFAEEADCLAGVKALATV
                     AADLTGGER"
     gene            13946..15262
                     /locus_tag="PKU75_00520"
     CDS             13946..15262
                     /locus_tag="PKU75_00520"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HPF79387.1"
                     /translation="MTTIWARHAQLPDGMAAKVRITIEGDRIRAVGRGEPQPDDISLP
                     LVLPGIANGHSHAFHRALRGRVQDAAGNFWTWRDHMYAAASALTPESYFALAHGVFVE
                     MVTAGFTAVGEFHYLHHGAGGRPYENPNAMGEAVIAAAQAAGIRLTLLDTLYLAGGLG
                     GEGHLPLDPVQERFADKDIATWARRFGDLRDTAGCRIGVAAHSVRAVPREALLQLASV
                     AGPRVIHAHVSEQPAENAACTLVYGQTPTQLLDNSGLLSERFTAVHATHLSRDDIRRM
                     GRARAFICMCPTTERDLADGIGPARDLLDAGARLSIGTDQHVSIDPFIEVRGLEMHER
                     LHSGERGRFTLDELNLAMSHHGYASIGWPDGGLIAPGALADLVAVRRDSVRTVGSRAA
                     QVMFSAAAGDVTDVMIGGEFVVRGGVHRAGDPGALLGKALMKLRGA"
     gene            15273..16445
                     /gene="hutI"
                     /locus_tag="PKU75_00525"
     CDS             15273..16445
                     /gene="hutI"
                     /locus_tag="PKU75_00525"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPF79388.1"
                     /translation="MTSALVTGIGQLVTCAGPGSTPEDALGIRTNAALVVEDDRIAWI
                     GAAADAPPADRRIDCEGRAVLPGFVDSHTHLVYAGDRAAEFEARMAGRPYDGGGIATT
                     VTATRAAGDDELRELLSSRMTELRRQGSTTVEIKSGYGLDVDGEARLLRLAREVTTET
                     TYLGAHVVPPEYAGDRAAYVELVCGAMLSACAPHARWIDVFCEPNSAHAFDGDEARTV
                     LTAGMAAGLLPRVHGNQLGPGPGVQLAVELDAASVDHCTYLSDADVDALAGAPQTVAT
                     LLPGVEFSTRSPYPDARRLLDAGISVALASDCNPGTCNSSSMPFMIALAVREMRMTPA
                     EAVLGATRTAAKALRRNDIGAITVGMRADLTVLDAPSYAHLAYRPGVPIARAVDLA"
     gene            complement(16450..16740)
                     /locus_tag="PKU75_00530"
     CDS             complement(16450..16740)
                     /locus_tag="PKU75_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HPF79389.1"
                     /translation="MGGMVWKALAAGAAVVAGLVANKSVTAIWSKAGKDENVDPQNPN
                     TPLGEALALAAVSALAAAVAKTFFTRKAAQYYANSAGHLPEPMRKKEEEAQA"
     gene            16764..17426
                     /locus_tag="PKU75_00535"
     CDS             16764..17426
                     /locus_tag="PKU75_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HPF79390.1"
                     /translation="MRWRRTALALFIGAAASGLGILAGSNIWVARASAGRMYAVADVP
                     AKPVALVLGAGLTPAGEPTPYLAFRLDLAKQLYDARKVEVLLVSGDNRTHAYDEPTAM
                     MDYLVAQGVPATRIVRDFAGRDTYDSCARATRIFGVTEAIVVTQEYHLPRAVATCRAV
                     GVDAVGVGDTRGQQYGATWSQGRLRERAAVVKASWDISIRRDPVLGAQESGVRDALST
                     LR"
     gene            17522..19609
                     /locus_tag="PKU75_00540"
     CDS             17522..19609
                     /locus_tag="PKU75_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HPF79391.1"
                     /translation="MSRSSAVSAVPVPSSPAQIRNVVLVGPSGGGKSTLFDRLVSGES
                     ALRRKEDDSASTTLRVATVGVDDLAVTLLDTPGAPDFVGEVRAGLRAADAALFVVAAG
                     ASVDEASRMLWRECAAVGMPRAVALTKLEQCQDDYATIVEACRRAFGDAQPLQVPILE
                     GDKVTGLLDLLGQSVHGPDGEVSGTPTGDQAAIIEDLRGGVVEAIIEESEDETALDSY
                     LNGDELDADTLTKDLKAAIATARFFPIIPTHWASGVGVGELLDLFATGFPSPADAWLP
                     EVTTPDAGEFGEIECDPAGPLVAEVVRTSSDQYVGRLSLVRIFSGTLTPDQQVHVSGH
                     LEHLTGEEVEGHPGHDDTDKIGGVQTPTAVDPIVRTSAIAGEVVLLTKLAHAETSDTI
                     SATDKPGLVQPWLLPAPLLPVAIHAASKNDEDKLATALQRLVAEDVTMRLEHNVETHQ
                     LVLWALGPAHVDDLLARLKDRYHVNVEVEPLKTSLRETFVRECDLQGRLVKQSGGHGQ
                     YAVVKVKIEPLERGAGIEFVDKVVGGAVPRQFIPSVEKGARNQLEKGVLAGYPVVDVR
                     VTLYDGKAHSVDSSDMAFQTAAATALREAANESTVSMLEPVDQIVVTVADDNVGAVMA
                     DLRGRRGQVHGTEPADTEGFTKVTAEVPQHELSRYPIDLRSVSHGTGSFTRTFVRYDY
                     MPAAQAKEVIANA"
     gene            complement(19657..20379)
                     /locus_tag="PKU75_00545"
     CDS             complement(19657..20379)
                     /locus_tag="PKU75_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HPF79392.1"
                     /translation="MSTALDVSEAFRFVDLAGVLCNGMIGATIARGRRFDLVGMIVLG
                     IVSGLAGGALRDIMLNVGQPVALTDPAYLVTAIVGVLIALVFRIEGRRWRWPLLIADA
                     LALGCWAATGTFKAQVVGLGWLPSLLLGVVTAVGGGMVRDICIGQVPAVLGGNTLYAT
                     AAAAASGAMLLVPVQSRLTWGMAAGIVIGGGLTLLARRLGWSLPAADPDAGVVLSRSQ
                     LRQLIRRSERAGARKAGRPPPP"
     gene            complement(20405..21583)
                     /locus_tag="PKU75_00550"
     CDS             complement(20405..21583)
                     /locus_tag="PKU75_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF79393.1"
                     /translation="MRRGAGPATGTLADDRVGGVMGAAPSDAADPVALDARVEVVHQG
                     ARPFALQAHVAAGPGEVVAVLGPNGAGKTTLLRAIAGLTPLSGGRIRLGDRTLDDPAL
                     GTWVVPEQRRLGVVFQDHRLFPTMSVRENVAFPARARGMRHGAALANADAQLAHFGLT
                     EIADRRPARISGGQAQRVALVRALASKPDLLLLDEPLAALDAQRRDTTRAELRAFVSA
                     FAGPALLVTHDPLDALLLAGRIVVIEAGRVTQDADPATIARRPATAYVARLAGLNHYR
                     GRVSRGGIELDGGGRLVAVDLPAQSSSGSREVLIALAPSAITVHTTRPDHGSARNVWA
                     GRVRGIQQIADRARLDVAGPPDALVDVTPAAILDLGLTPGSAVWLSAKATEVTAYAAE
                     "
     gene            complement(21523..22308)
                     /locus_tag="PKU75_00555"
     CDS             complement(21523..22308)
                     /locus_tag="PKU75_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF79394.1"
                     /translation="MRTPWQLRVPAAVAAAFLVLPLVALLFRVPWLHLPDLFREPIVG
                     EALRLSLLCATIAALLAAVTGIPLAWLLARSTGRLTGLVRALVTVPLVLPPVVGGVAL
                     LSAFGRRGLLGKPIDVVFGFSLPFTPFGVIVAETFVALPFLVLTVEGAFRTLDDGYGE
                     AAATLGASPWFAFRRVVLPMMAPALLAGTVLAWARALGEFGATITFAGNSPGRTQTMP
                     LAVYLAMERDPDAASALSLVMLVVCVVVLALLRGRWLTTGSAA"
     gene            complement(22389..23207)
                     /gene="modA"
                     /locus_tag="PKU75_00560"
     CDS             complement(22389..23207)
                     /gene="modA"
                     /locus_tag="PKU75_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPF79395.1"
                     /translation="MRALARLTPVVILAAVALAGCGDSSSGSSSAGSTGAGSASGSGS
                     GPVASGSLTVLAAASLRDVFAKLGDQFEADHPGSTVEFSFGPSSGLATQIVEGAPADV
                     FASASAKTMKTAEDAGAVNAPVTFATNSMTIVVPKDNPGTVDDVSDLASKDTTVAVCA
                     KDVPCGAAALTVFEQADLDVTPVSEEVDVAAVLTKVSLGEVDAGLVYVTDAALARDKV
                     TQITLPADVSATTDYPIAVTTESANPTLAQQFVDLVTGTIGQDALKAAGFAPAP"
     gene            complement(23210..23620)
                     /locus_tag="PKU75_00565"
     CDS             complement(23210..23620)
                     /locus_tag="PKU75_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPF79396.1"
                     /translation="MPHFRFKEAADLLGVSDDTVRRWVDQGRLRASLDASGRQTVDGA
                     ELAAYARALAAEMPRPQPRPVVSESARNRFVGLVTRVVKDTVMAQVEIQSGPHRVVSL
                     MSREAADDLGLEPGVLAVASIKSTNVVIELPEAP"
     gene            23692..24615
                     /locus_tag="PKU75_00570"
     CDS             23692..24615
                     /locus_tag="PKU75_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPF79397.1"
                     /translation="MATVSFPSKGWVRDVGPIEGITPVVWHPDNPPPDGPLDVVVAPY
                     VGAAACIASVDKVPGVRLVQLLTAGFDGIFERVPDRIAIANAAGVHDDSTAELALAMI
                     LASQRGIPDFVRAQERGVWLRPGFLPSLADRRVLVLGYGGVGRAIVERLLPFRARVSV
                     VATTARDGDDLVKRVRGMDELDRLLKKSEVVILAVPLNDTTRGLADDAFLSKLPDNAL
                     VVNIARGPVLDTDAALHHTDRLRFALDVTDPEPLPPEHPLWQAPGVLITPHVGGPSTA
                     FRPRAVEMLREQLGRIGRGEAPDHIVRPAAP"
     gene            complement(24768..25820)
                     /locus_tag="PKU75_00575"
     CDS             complement(24768..25820)
                     /locus_tag="PKU75_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79398.1"
                     /translation="MSVPSLALSPLSLSRKGVRRAGVTVVAAAATLAVAAGPVAAAPT
                     PSDRLAGRTAAGWLNRQFVDGTHLQTDFGGTAYDDPGLTLDAVLAFAAAKTNKVTSDR
                     AMSWIAQPATLGAYVGTGGESYAGAHAKLALAIDVTGRDVRSFGGRNIIAELAALQTS
                     SGRLSDKSEWGDYSNAFGQSFGVLALQRAGLSGRAGWAAGYLQRQSCADGSVPIAFDQ
                     SPCAGDPDATALAIQAFAATGRSGAAARAGAWLAQYVKSPGVTNSNTAGLVAAGLDRT
                     AYGAAADTARRVVRSNQLGCAAPFSRRAAIAYTRPFDAAAAPRATAQAMLGLAGADLA
                     TLSAKGSIAYAPTMAC"
     regulatory      complement(25882..25953)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(25987..28452)
                     /locus_tag="PKU75_00580"
     CDS             complement(25987..28452)
                     /locus_tag="PKU75_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF79399.1"
                     /translation="MTTTAAPIAALPAPVDAPSWAAATWPLGAHFDANSSSTTFAVTA
                     PAATRVQLEIYARATGAGPVAVVDAQRGPDGTWRARLTGVGPGAHYGYRVWGPGWTVD
                     PAWTPGSAAGFVADIGPGGERFNPNKLLTDPYAREISHSPASPEVAAAGADAGIFGWG
                     PRDYRGRPLREWETAAYAPKAIVVADATPTGTKPKVAAQDAIIYEAHVRNLTSHPSAT
                     QLHTLLSGLPRFAGVGDVPAHLQGTYAGAALLAPYLKALGVTTLELLPVHETSGSELD
                     TAGSSNHWGYMTLGFFAPNRQYAADKSPGGPTREFKAMVRAFHDAGIEVYLDVVYNHT
                     GEGGNWGDGDTVGFTSFGGFATPDYYVLTDTGLLVDGATGCGNQTDLSSPVAQRLVLE
                     SLAYWCDEMGVDGFRFDLAPVLGRLPDAAPREAWGEQRRFHSEHPMLTAIRDLAAARD
                     IEVIAEAWDLWGYEVGNFPDGWGEWNGRYRDAVRRYLKGDGNTGTFMAMMNGDYQGFR
                     DQGGPQRSINFVTAHDGFCLLDLVSYDGKVNGQPWPFGPSDGGSDSNDSWGSGGNQTF
                     VRQRLRNFMVALILARGVPMMCSGDEFARTQNGNNNPYNLNTIGMWNNWAAAATNAPT
                     QAAVGVEGAAYHDTFGVAAAPAGVNPHLVFTTYLTALRRSHPGLRQRSYGDAELGGTD
                     VSYVFTQPQSADAPTDGDRAVRLWIDASPVGGSDLLVLINMADQAVRFGVPAAAEGVA
                     WHRIVDTASHFEAGYNCWALEKSDVIVGDYAAAPWSIVVLSEGAPGVESAANSPDGPP
                     ASSIVTEVADTIIDTVRDWLRRR"
     gene            complement(28498..28923)
                     /locus_tag="PKU75_00585"
     CDS             complement(28498..28923)
                     /locus_tag="PKU75_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPF79400.1"
                     /translation="MSRDIAELVSSVGVRLRERGERMTEARRAVLVALATTPGHLTAD
                     DIHARAGADLPASSVYRALEALSGVGVVQHIHLGHGATAYHLTDGDDAHVHLQCRSCG
                     AVLDLPAEILDPVAAYVARAGFELDPTHTALSGSCAACR"
     gene            complement(28920..30413)
                     /locus_tag="PKU75_00590"
     CDS             complement(28920..30413)
                     /locus_tag="PKU75_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79401.1"
                     /translation="MSPSPPPPPPPGWRRAAPAPTPQEPEWGDEDWGYEPDPEPVAPP
                     GPPTRTQRVGQAVRRGSQGAARVVGAGTRAGTRRYQAFATAHGADATGLARLIQLHTA
                     NVAGDAALTVSLAGTVFALPTDEARSQVALFLALTMVPFVLLAPLIGPLLDRFNHGRR
                     WAIGTTLALRAFLAWVLASAIVDDSVWLYPTALGALVASKSYAVTRAAAVPRLLPQGI
                     TLVKANSRLTIAGLLGMMAGGGIAGALSRIGPDWSLRAAFVLYIVATVLAIRLPSRVD
                     SDRGESDFAGDEAASPTRRLRALPVSVRYVLWLLTGARMLSGFLTLFLVFLMREHPIP
                     GISGSLVVGLVAGAAGVGNALGSFIGNRKHNPPPELLATILLGVAVAAAVTATFVYAV
                     WSLVALGLIAGLFGQLGKLNLDALIQRDVQDRLRGRVFAWSETLLQTFWVIGGAAGIV
                     VPLQPRLGFAVVSGAIILALLAALRSRSTGQGRASVGESAPDLAGGR"
     gene            complement(30707..32299)
                     /locus_tag="PKU75_00595"
     CDS             complement(30707..32299)
                     /locus_tag="PKU75_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79402.1"
                     /translation="MVALFAGLKLRLIRNRAMNRGDLGILWLILGVLAAIVVGGLVGL
                     GFATLRAAPDAAPLGLASFFFSLLLAWMVMPLVAFGVDETVDPRRFALLPIPLGRLQR
                     GLLVSSLVGYLPLANAIALTGAAIGLSRSWSLLPVALACVLGVLLMCVIGSRALAASM
                     ASLMGSRRGRDLGMAVTFALFVVYVVLSGAAGGARAGSFADSLAGSVGRVLGWTPPGA
                     LAVIPSDLDEGRWLSAAVRALIAVAFLVALWVWWGRALHKSLTTIGSTTESSAVAGGL
                     MDADAVATDTRSTAQLMVGRDATMTWRDPMRRLPLLMIVMLAVGWPFLVTGSERSGYA
                     VAFAAAMAGMQAATSYATEGSGLWLHLVAFADRARARGELWGHAIAAIVPGLVISLVG
                     VLIQVVVRDGWSHLPGMLGLCFGAVIASTAAALFASAYVPYAVPQSRTSLFASRVPGQ
                     GGRAFAASLMTMLGGAALCIPVIVLVVLGETVDSMYAWATLVLGPLYALVLLWFSVRF
                     SAERYLSRAPEILQTVSAGDRV"
     gene            complement(32275..>32985)
                     /locus_tag="PKU75_00600"
     CDS             complement(32275..>32985)
                     /locus_tag="PKU75_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79403.1"
                     /translation="VNGINLDVPAGSFYGLVGPNGAGKTTTLSMVTGLLRPDQGQVWV
                     HGVDVWADPVAAKKAIGVLPDGLRLFERLSGAELLSYLGRLRGIPTEVVTQRSGELLD
                     VLDLTGAGDKLVADYSTGMRKKITLAAALLHSPPLLLLDEPLEAVDPVSARIIREVLH
                     SYVRGGRTVVLSSHVMDLVEDNCTHVAVLNEGNIVAAGPLAQVRGDAASLDDAFFHIV
                     GGRDIAEGGLEWLRSSQD"
     assembly_gap    32986..33085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<33086..33861)
                     /locus_tag="PKU75_00605"
     CDS             complement(<33086..33861)
                     /locus_tag="PKU75_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPF79404.1"
                     /translation="MPAFRQIDVFTAVPLRGNPLAVVHDADGWSDAQMARFANWTNQS
                     ETTFLQQPTDPRADYRVRIFTIAGELPFAGHPTIGSAHAWREAGGIPQGEGRILQECA
                     VGLIEVSYAERLAFAAPPLLRSGPVEPERLRSLGEALGLTPDHILDAAWIDNGPGWIG
                     LEIDSAAHVLALDPDPTRMRGLKVGVLGRHDDSGPDGVEVEVRAFYSDGRDAGEDPVT
                     GSLNAGMAQWLIGEGRLPERYVARQGTALGRDGRVHVHTT"
     gene            complement(33886..35202)
                     /locus_tag="PKU75_00610"
     CDS             complement(33886..35202)
                     /locus_tag="PKU75_00610"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HPF79405.1"
                     /translation="MPTLPIAARLGAGLLTIPVFAAGTTATASIPEGGRLDGASIHVA
                     TTGSDANPGTAAKPLRTIQKAVDRSGPGAKILVHKGTYNQQISITHSGTARAPITLTA
                     AGDGPVKLTSVQPATKCSSMKPTSDRTILVKGGADYWTISGLQIHNGVYINGGGGFDA
                     FTWTDSLVDKRNWQERRRVPGTSTNDPGASKGAIAYVESKIGRTLNPADGIQLIGNTV
                     TGRGIHATLARSGLIQDNTITRVDCGTGPGIWVLTFSTFWRITGNDVSHIADASTNHY
                     MQEGIRLGTSSNYNRVDNNYVHDLGDDGRGFATDVDASFNIFENNLVRDVPVGYNDEM
                     SGWGNVWRNNRADRYTTIAFGFRMKDRELTLPSKDTSTHYSIVTNNVATNPKGSTAYG
                     LGIGAIAGSTFSGNRLGRVVLGKYLKGYWAKQGNTWNGTTRPPSNS"
     gene            35430..>36200
                     /locus_tag="PKU75_00615"
     CDS             35430..>36200
                     /locus_tag="PKU75_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79406.1"
                     /translation="MASSKLGKGALAASAAGLLIAGGGAIAYAATGDSTSTTTPTSTA
                     SSSTSSGLPSLDNGQGANNGQNNGQTGQQAPQGGMGRGGMGDGQGMGGHAHTAVTGDE
                     LTKVKDAIKAKDSAVTITQVMKDEDGSYDAFGTKDGQMVRVEVSADLKTVEVGRGGMG
                     RGGMGDGQGMGGHAHTAVTGDELTKVKDAIKAKDSAVTITQVMKDEDGSYDAFGTKDG
                     QMVRVEVSADLKTVEVGQGGPGMGRGGMGGHHGGGMPGQ"
     assembly_gap    36201..36300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            36359..37429
                     /locus_tag="PKU75_00620"
     CDS             36359..37429
                     /locus_tag="PKU75_00620"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HPF79407.1"
                     /translation="MPEPLADQFRGHFGHRQHLYGVLLDHLADDLEAGGPTAEVCRDQ
                     LDAVRADAIQLRLLAAVFRIVLRGEAPQLTPFYACLGGTAPAEQCWPVLRAVLPDHVA
                     ELRAGLTRPPQTNEVGRSAVLVAGLFEAVRRTGLNRVRLLELGASAGLNLHPDRYRVS
                     GPGWTWGEPDSPLALDTEAADIRPLDLTIVRRGGCDLDPVDVTTQEGRIYLTSFVWPW
                     QLERHARLAAALAVAQAHPVRVDRAPASEWVAGQLNRPPTPGVLTIVWESITRQYWPA
                     HESADVDAHLAEARRRMPLAHLSMEGVPPRQGTDGYDIRRDGPVLRLDGEPLARSHHH
                     GPPVLLENVISETFTGVRSIPP"
     gene            37446..38690
                     /locus_tag="PKU75_00625"
     CDS             37446..38690
                     /locus_tag="PKU75_00625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HPF79408.1"
                     /translation="MSTVVILGAGTAGTMLANKLRKALPRDWSITVVDKDDEHHYQPG
                     YLFVPFGTYTPERVVRSRKAQLDRGVDFVIDAVAHVDPVHQQVRLASGRQLSYQWLII
                     ASGTVPRADQTPGLLDERVWRTSAFDFYTLDGAAALQDALESFEGGRLLVHITEMPIK
                     CPVAPLEFAFLADDYFRKRRMRHKVDITYVTPLDGAFTKPVASAALGDLLEDKSIRVA
                     PDFAISHVDPEKQAIVGYDGRELPFDLLVTVPLNMGQQFIIDSGLGDDLGFVPVDKHT
                     LRSQISDRIFAIGDATNVPTSKAGAVAHFEVEHLVKNLEAAIAGAPLPSKFDGHANCF
                     VESGHGKALLLDFNYDTEPLRGTFPASKVGPMKLLGESRLNHIGKLAFEKIYWNMLLP
                     GKPLPVPTEMSMAGKIPAGHRS"
     gene            38696..39013
                     /locus_tag="PKU75_00630"
     CDS             38696..39013
                     /locus_tag="PKU75_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPF79409.1"
                     /translation="MPTNTIDGHTFAVNDEGFFTNREEWSEDLAVILADLVGIEQMTE
                     AHWAPLRFMREDSAKTGVTPTLRRMQAVGGFDVKDLFALYPGKPAKKMAYLAGLPKPV
                     GCV"
     gene            39035..39649
                     /locus_tag="PKU75_00635"
     CDS             39035..39649
                     /locus_tag="PKU75_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPF79410.1"
                     /translation="MRTLPEGFIIPDFGGSAAKATTTSPEPAAGGEATAYRGPRKLAL
                     ICSKGNLDMAYPGLILGNAAAGEGVEVHMFFTFWGLDIVNKKTHKNLKFTMLGNTAMH
                     MPELGYLRTGLEHASMPQLMGQLPGMTAMATKMMKKQMADLEIPDVPEFLDLLSAAGV
                     HMYACKLSFDMMKMIEADLHPGVEGVISAADFIEISEGAQTIFI"
     gene            39693..40796
                     /locus_tag="PKU75_00640"
     CDS             39693..40796
                     /locus_tag="PKU75_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF79411.1"
                     /translation="MDIRDLRPPVEDGPWPPLTQLPPMTFEPLARATSWQQLIYAQGG
                     GFRPLLMDVHVPATPRPAPVVLWVHGGGWGEGDRRLVPLQWGQQEFFQQFVDAGIAVA
                     TCDYRLIAEAPVEAMVQDIVAAIRYLRRYAGELALDADRVAVWGDSAGSHLSSLAALA
                     GSAPDPDPAILGSQGVGEGRADVTGIVWWYGASDLTVLPELCEFLWATATPEERTTLA
                     ERYSPVTYLRADSPPLLIMHGDQDTMAPFDQAVRLHEAAVAVGAPSRLLVVPGAEHAF
                     LGADIRAQWQTGLDFLAGLFAGGRAPAAARACSSLGKRSPDACPRRVVDHRNDAGSAH
                     DHPGRQAPGRVAPNTDQAYRVWRIAEHNGRQPP"
     gene            40777..40899
                     /locus_tag="PKU75_00645"
     CDS             40777..40899
                     /locus_tag="PKU75_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79412.1"
                     /translation="MVGSHHRLRTNDVLALRRMRLEQRRAAALELLDLEDKLGL"
     gene            41043..41933
                     /locus_tag="PKU75_00650"
     CDS             41043..41933
                     /locus_tag="PKU75_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79413.1"
                     /translation="MTSRHGFSGRRRHGAAAVVALLVLGGCGTATGRTSSPPVTSAAT
                     SATVVSVGGVTDVSTYATWADPRITESSSLTRVGDHLITVNDSGDPAVLYVSTGTSGQ
                     ITGTITYADAAPDDVEALATSPDGTIWVGDIGDNARARSTIVIYRLPPITQWDGDTTV
                     TAQRYEFAYPDDAHDAETLLVQPDSGDLFVVTKDASGGVVYAVPTLDATRTVTLEKVA
                     QVAATVTDGVFDASGEHVLLRTYGEVTAYGYPGFQQVATATLPAEPKGEGLTLGPDAG
                     TYLTATEGAGTPVLRWRWSV"
     gene            complement(41937..>42216)
                     /locus_tag="PKU75_00655"
     CDS             complement(41937..>42216)
                     /locus_tag="PKU75_00655"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPF79414.1"
                     /translation="GDPMEIAACIPQTEGLPLLPGTEHVELAAVPRAITVVHRGDVET
                     IGDTWQALQQYVGAQGLSNTGRCREVYIATPMDRPDDWVTELQQPVTA"
     assembly_gap    42217..42316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<42317..42538)
                     /locus_tag="PKU75_00660"
     CDS             complement(<42317..42538)
                     /locus_tag="PKU75_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPF79415.1"
                     /translation="MLSIGDFALHGQVSVRMLRHYDALGLLEPVHVDPSSGYRWYAAE
                     QLSRLNRLIALKELGFTLEQIGPILDASVS"
     gene            complement(42609..43343)
                     /locus_tag="PKU75_00665"
     CDS             complement(42609..43343)
                     /locus_tag="PKU75_00665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF79416.1"
                     /translation="MSAGALSGKTAAVTGAGAGIGRASAEAMIGAGARVAIIDRDENL
                     LNKMKDEHGEAVVPIQADLLDTAQCAGLIPRVLESFGGKLDIFHSNAGIYVGGDLTEA
                     SNEAIDRMLHLNVNVVMKNVHDVLPHMIEAGGGDILVTSSLAAHYPTPWEPVYASSKW
                     AINCFVQTTRRQYFKHGIRVGSVSPGPVASALLSDWPEDKLREAKESGSLMEPSDVAE
                     VILFMLTRPRNVTIRDVILMPTNFDL"
     gene            complement(43415..44758)
                     /locus_tag="PKU75_00670"
     CDS             complement(43415..44758)
                     /locus_tag="PKU75_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPF79417.1"
                     /translation="MTAPVSALPQARERILILGAAGRDFHDFNVIYRDDPAKEVVAFT
                     ATQIPDIEGRTYPPELAGSLYPGGIPIYEEARMEELIENERIDTVALAYSDLPHEQVM
                     HLASRAIAAGANFLLHGADSSMVESTKPVIAITAVRTGVGKSQTTRYLSKILADLDQR
                     VVAIRHPMPYGDLAKQACQRFATYEDLDLHECTIEEREEYEPHIDNGFVVYAGVDYEQ
                     ILRAAEDEADVILWDGGNNDIPFYKSDLHIVLTDPLRPGHEATYHPGEANVRMADVVI
                     VAKCDSAAPEDIEAVEASVRQMNPTATVLRCDSPVTVDDAEAIAGKRVVVVEDGPTLT
                     HGSMSFGAGVVGAKAAGVAEIVDPQPYAVGAIAATYAKYPNAAGILPAMGYGEGQVDD
                     LQATIEAMPVDAVVAGTPIDLSRVLTVEKPLVRARYSLREQEPGGLAEAVRAVVP"
     gene            44959..45450
                     /locus_tag="PKU75_00675"
     CDS             44959..45450
                     /locus_tag="PKU75_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF79418.1"
                     /translation="MAHTLLVLRHAKSDWAVPVSDRERPLAKRGVRQAPHTGRWIADN
                     DLVPDVVIVSPATRAIRTWELVCGAWTARPTEVRIDEAAYTFGAGGLLGLVRATPESV
                     ARLALVGHNPAVEDLVAVLTDESVRMPTSALAVVDLDRWQGAGMRDGCLRYAGRPADE
                     SGF"
     gene            complement(45473..46660)
                     /locus_tag="PKU75_00680"
     CDS             complement(45473..46660)
                     /locus_tag="PKU75_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HPF79419.1"
                     /translation="MSSLQADRRVVRQIRHDVSERPFIVIWEVTRACQLACAHCRAEA
                     IHQRNPLELSTTEGKALFDDIAGFGKPYPIVILTGGDPFEREDLAELTAYGHSLGLHM
                     ALSPSATPNVTAERLAAMREAGASAMSLSLDGATPAVHDAFRGFGGTWQRTIDSAGLI
                     NDAGFRLQVNSTVTSRTVAELPKLLALLLDLKVHLWSLFFLVPTGRGKALEALSPDQV
                     EDVLHWMVDISHTMPVKTTEAQHYRRVVVQRAAAEKEGHGAEAFATGQLYAALTQRTS
                     EVLAGRELPTRPARPPIDTNSGRGFVFVDHQGWVYPSGFLPQRAGNVRDEGTTFREIY
                     REAPILKALRDPDHFAGKCGVCEFNQVCGGSRSHAYAVTGDPLASDPTCAYLPPRWRE
                     AAV"
     gene            complement(46709..47104)
                     /locus_tag="PKU75_00685"
     CDS             complement(46709..47104)
                     /locus_tag="PKU75_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF79420.1"
                     /translation="MTDTPETDRWARKDFWGVVLDAPEVAPLLNFYSKLRGWPIYKAD
                     EEDGALDLGEGVAYLAVQRNPDYVRPVWPTPPGGQQMQSHLDFEVEDLDAEVERAIAL
                     GAELAAYQPQDDVRVLLDPAGHPFCLYVS"
     gene            complement(47101..48000)
                     /locus_tag="PKU75_00690"
     CDS             complement(47101..48000)
                     /locus_tag="PKU75_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPF79421.1"
                     /translation="MSGGHAHGGSAAAHHRWRLAISFALISGFFAVELIAGLVSGSLA
                     LISDAGHMAADVVTLGAALVATRIATRPDTTGRRTFGSYRAEVFASGIAVLVMLGVAV
                     YIVIEAVQRADHATAPALQAMLLVGALGLIVNLVCLALLHSVAGESLNVKGAYLEVLG
                     DTLGSVGVIAAALLIEATGDSRWDTVIALAIALFVAVRALLLGREVLAVLGQHAPAGM
                     EPREILAALEGLDGVAEVHDVHVWQLTSGMNVATAHLVRAGDAAGDPLAAATALLRDR
                     FGIAHATIQVETTPGTDCEGADW"
     gene            48162..49571
                     /locus_tag="PKU75_00695"
     CDS             48162..49571
                     /locus_tag="PKU75_00695"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HPF79422.1"
                     /translation="MTTLHTMGQQHSRRSWAEPYKIKVVEPLRTTTREERDRAIAEAG
                     WNTFLLHSRDVYIDLLTDSGTSAMSDRQWAGMMLGDEAYAGSVNFYRLEETVQKYYGY
                     KHLVPTHQGRGAENLLSQAAITPGDHVPGNMYFTTTRFHQEKAGATFHDVIIDEAHDT
                     ASLHPFKGNIDLDKLQALIDEVGADKIPYVCMAATVNMAGGQPISMENARAVRELTDR
                     HGIKVYLDATRAVENAYFIIEREAGYADKSLAEVLAEFCSYSDGATMSGKKDHLVNIG
                     GWFATNDDELFEEVRNLVVVYEGLHTYGGMAGRDMEAMAIGIEESLDMPYMRSRIGQI
                     QYLGELLEQWDIPFVKPVGGHAIFLDARAFYPHLDQEQFPAQTLAANLYADSGIRSME
                     RGIVSAGRDPETGEHRHPKLELTRLTIPRRVYTQAHLDLVAESVKACFDARDSATGLR
                     LVYEPKYLRFFQARFEPLP"
     gene            complement(49597..49893)
                     /locus_tag="PKU75_00700"
     CDS             complement(49597..49893)
                     /locus_tag="PKU75_00700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79423.1"
                     /translation="MSDVVVTNNPAQDRYEAHLDGELAGFAEYILSADLIVFTHTEVD
                     DKFEGQGVGSALARFALDEVREGTRRVKPQCEFIKSWIQRHPDYADLVARGEHH"
     gene            complement(50012..50465)
                     /locus_tag="PKU75_00705"
                     /pseudo
     CDS             complement(50012..50465)
                     /locus_tag="PKU75_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
     gene            50492..50761
                     /locus_tag="PKU75_00710"
     CDS             50492..50761
                     /locus_tag="PKU75_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79424.1"
                     /translation="MPLRPPRGYAGVAGSGGLDGLLADPGIQALGADATRLTLGLAVW
                     AMIMGAVTAEVFSQYGTQAISDPAAHFAEVVELAVGVIAGGQTGR"
     gene            50792..51154
                     /locus_tag="PKU75_00715"
     CDS             50792..51154
                     /locus_tag="PKU75_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79425.1"
                     /translation="MADSPEDYLAAAPEAGRPWLTEFWDHVRRRSPELELAMFRGVPM
                     FKFERSYQDGYVMFTAAKGHFSAHSLAFELIEETKAEIPGSFGGKGCVSVKYANEEAK
                     PALKAYVDEVLRAASAED"
     gene            51154..52590
                     /locus_tag="PKU75_00720"
     CDS             51154..52590
                     /locus_tag="PKU75_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF79426.1"
                     /translation="MPTAYVVGSGPNGLAGAVRLAEAGLDVTVLERADRIGGGMRTSE
                     NTLPGLLHDDCSAFHPTGAASPYLQSLGLERHGLQWLWPEIQYAHPLDDGSAGLAFRS
                     VESTAEGLGRDGASWARLFGPLARQYDALAAELFRPVLHVPRHPLALARFGPAALLPA
                     TVLARRWQDPQAKALYGGVAAHLLGRLDRPVSAAVGLMLGAAAHAYGWPVAKGGSQAI
                     ATALAARLTDLGGRIETRAEVTAYDQIGDADVVLFDLSPPAVARILGERLPQRAAAAY
                     RRYRFGPGAFKVDLAIEGEIPWRNPDVGRAGTVHLGGTLEETAAAEADVVAGRMPWRP
                     FVLLGQQYVADPTRSAGGLNPVYAYAHVPAGWVGDATDAVVGQIERFAPGFRHRIRAT
                     FTRGPAELEVYNPNYVGGDIATGRNSPVQVVMRPRISLHPYRTGAAGAYLCSAATPPG
                     AGVHGMGGFHAAETALADLEPRRERGNA"
     gene            complement(52604..53356)
                     /locus_tag="PKU75_00725"
     CDS             complement(52604..53356)
                     /locus_tag="PKU75_00725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPF79427.1"
                     /translation="MRQLIEIPVATERVEAIVATPDETDAHPGVLLFMDAFGLRPQIE
                     AMADRIASWGYVVLAPNTLYRSGRVAEVSPPADLRDPDTVAKVFARVMPMIGRLDGAA
                     AAVDVPAYVAALRALPQCAPGAIGVTGYCMGARLATRAAGLDASIAACGGFHGGRLAT
                     DDPDSPHRGLAHARAAFVYGHADEDAGMDAEAQSRLDAALAEAGVRHTTAVYPGARHG
                     FTMADTGAYDAQAAQRHYAELEALLRDTLERP"
     gene            53542..53943
                     /locus_tag="PKU75_00730"
     CDS             53542..53943
                     /locus_tag="PKU75_00730"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPF79428.1"
                     /translation="MPADLIRSGLLSDVAQYAYAAVVPEGTRLVFLAGSCPLDESGVT
                     VGTRDYLRQAEQCVANLRTVLGELLADITDLVSTRVLVASTDQQDLGAAWHGYRAAIA
                     PHDPPSTLLGVTVLGCDHQLVEIEAVAAIRC"
BASE COUNT         8290 a        18679 c        18744 g         8016 t
ORIGIN      
        1 gacactgggg aggtcatcgc ctcagcgccg gccgggatga gcgaactctc actctcccag
       61 tgggtcctgg acaagcgcta gagcccgcaa ccacccccgg ctcaggggag gagcaggagc
      121 ttgccgatgt gctggctggc ttcgaggtcg cggtgggccc ggggggcgtc ggcgaggggg
      181 taggtcgcct ggatgaccgg gcggacctcg ccggcctcga tcaggggcca gacgtgctcg
      241 cggacggcgg cgacgatcgt cgccttctcc gccgccgggc gggcgcgcag ggaggtggcg
      301 atgacggcgg cccgcttggt cagcagggtg gagaggttga gttccccctg gacgccgccc
      361 tgcaggccga tgacgacgag gcggccgttg acggcgagcg tgtcgacatt gcgggacagg
      421 tatttcgcgc ccatgttgtc gaggatgacg tcggcgccgt ggccgtctgt ggctgccttg
      481 agccgctcga cgaagtcctc ctccttgtag tcgacgagga tctcggcgcc gaggtcgcgg
      541 catacctcca acttctcggc ggagccggcg gtcacggcga cgcgcgcacc gacggcccgg
      601 gcgagctgga tggccatcgt cccgattccg gacgaaccgc catggaccaa aatggtctct
      661 tccggttgca gattcgcggt caggaagaca ttggaccaga cggtggacgc aacctccggg
      721 agggccgccg catgctgcag tgagacgccc ggcggcaccg ggagaacctg accagcgggg
      781 acggtgacca actcggcata cccgcctccg gacagcagcg cgcagacctc gtcgccgacc
      841 tgccagccct tgaccccctc gccgaggctg tcgatcgtgc cgctcacctc caggcctggg
      901 tatgccgagg cgcgcggcgg cgggggatag aaacccttgc gctgcatgac atccgctcgg
      961 ttgacgcccg ccgcggcgac gcggagccgc acctcgccag ccgcgggctg cgggtcgggg
     1021 acctcggcca ggacaagagc ctccgggtcg ccgggcgtgg ggatcgtgat cgcgcgcatg
     1081 gtgacgatcg tagaagggcg acgccggtgt gggtcggata ccctaggggg catgctgtcg
     1141 acctggtccg caaggcgctc gctcgtcgcg tccgtcgcga cgctggccac gggactgttc
     1201 atcagcctgg gcacggcgat gatcgcgaac cccgtgatcg gccgctcgat cccgccgacc
     1261 tggtggtcct ggccggtcat ggcggtcacg gccctcctga gcggactgct cttcgcgacc
     1321 tatgtccgtg agccgggtgc ggtgcccgcc gagtcgcagg tgaaccggcg cggagccgtg
     1381 ggcggggcct tgtccttctt cgccgtcggg tgcccggtct gcaacaaggt ggcgctcctc
     1441 ctgctgggct cggccggcgc ggtgcagtgg ttcgctccca tccagcccta cctcgggatc
     1501 gccggcatcg ccctcctcgc ctgggcattg cgcacccggc tggcgggtga gcgctcctgc
     1561 ccgctcagcg tccccggccc cgggccggcc tgagggcggg gcggcgggga cacgcggcat
     1621 atctacggcg ttccgagcgt gacgagcgtg caccgcaccc cgggggagca ggagaaccac
     1681 catggacaag accctcatca gcctcggcgc cgccctcgtc gccgccggga tttccgccgt
     1741 gcccaaggcg cgggccgccg cgaccctcac cgcggccggc ctggtgccgc tcgcggccgg
     1801 catcatcgcc aacgcccgca aggccgccca ggagcgctga cgcaccctca gcgtcatacc
     1861 gacgtcgggc ggtgactcag cccgcggcct cgagcgcccg gagggcctgc agcgcggcga
     1921 cacagtgcgc cgcctcccgc gcggtgtcgg ccccggcgtg cagggcgcgg tcctcgacat
     1981 aggcgaccca cgcgtgagtg acgcccgggt cgaggccggg gagcgcgcgg cataccagtt
     2041 cggcgatgcc ggcgcgcagc gaggtggccg tcgcgcccgg gtcgtcctgg tcgtgcagcg
     2101 gcccggcttg tccgtagacg agcgcgagat aggacgcgcg ccggcgcacg atctgggcga
     2161 tgagacgctc gacgatctgc cggaccgcgg gggcaccggt caggcccgcg tcgggccgca
     2221 ccgtgcgcag cacgcgtcgg ccggccgcct cgacgaccgc ccggtggtat gccgtcttgc
     2281 tggggaagta gtggaagagc aggccgcgcg agacgccggc ctccgccgcc acctcgtcga
     2341 gggagagttc gagcgccggg cgctcgacga acttgcgcag gccgatgccg accagttggc
     2401 ggcgccgctc ctcgggggtc agacgaaccc gactcgtcgt gtgagctgac cgactcggca
     2461 tgctattgag cattgctcaa taggacgcta aggtcaagca catggacttc gccccgtcac
     2521 cgcgcgccgc cgatctgacc gcccgcgtcc gtcagttcct cgacgagcgc atcgagccgg
     2581 tggaagggga gttggtcgcc gacatcgagg cgcgccgggc gcgcggcgag gattcgtggg
     2641 cggtcgatcc gcggatcgag gaactcaggg ccgccgcccg cgcggagggg ctgtggaacc
     2701 tcttcctccc cgccggccac gaggggccgt atgccgcgaa gttcggcacc gacggcggca
     2761 ccgggctgac caatctcgac tacgccccgg tcgccgagca gatgggccgc tccttcatgg
     2821 ctccgctgat cttcaactgc aacgcgccgg acaccggcaa tatggaagtg ctcttgaagt
     2881 acggctccca ggagcagaag gacacctggc tggagccgtt gctggacggg cggatccgct
     2941 cgaccttctt gatgaccgag cccgccgtcg cgtcctccga cgcgacgaat atggcggcga
     3001 cggcagccgt cgacggcgac gaggtcgtgc tcaacgggcg caagtggtgg tcctccggtg
     3061 cggggcatcc ggactgcaag gtcggcatct atatggcggt cacggacccg aatgccgatc
     3121 gccaccaccg gcattcgatg gtcgtagtgc cgatggatca ccccggggtg aaggtcgagc
     3181 ggatgctgtc gaccctcggg cagttcgacg agccgctcgg tcatggcgag gtgtcgctga
     3241 ccgatgtgcg cctgccggtc gaccacatca tcggcggacc gggacgggct ttcgagatcg
     3301 cccagggccg cctcgggccc ggccgggtgc accactgtat gcggctgatc ggcttgtcgg
     3361 agaaggcact tgagctcgcg ctcgagcggt cgacgacgcg cgtggcgttc ggcaagcctt
     3421 tggcgaatct cgggggcaac cgggagaagt tggcgaaggc gcgcatcgcg atcaaccagg
     3481 cgcgacttca tgtcctgcac gcggcatgga agctcgacac cttcggcacg gccggcgcgc
     3541 tctccgaggt cgccgagatc aaggccgccg tgccgtccat ggcgtgcgag gtgatcgacc
     3601 tggccatcca gttgcacggc ggcggtggca ccggtcccga cttccccctc gccgcggcgt
     3661 atgccggggc gcgttcgctg cggctcgcgg acgggccgga cgaggtgcat ctgggagtca
     3721 tcggccgaat catgttggcg ccgtatgccg ctcgcgccgc ccagcgtgcc gggcagagct
     3781 ccggcggggt cgcccggtga gtcgtgagac ccgacgcgtc ctgatcaccg gcggcgcgtc
     3841 cggactcggg ttggcgctcg ccaaggcctt cctcgcgcga ggtgacgagg tcctcgtcgg
     3901 cgacctggcg gacgagcggc cggacggcgt gccggcggag gcggcatacc gcaagctcga
     3961 cgtgcgttcg caacaggact gggacgcagc gcttgtggag gtcaaggagc gctggcgcgg
     4021 tctggacgtc ctcgtcaaca acgcgggtgt cgccaccggc gggcggatcg acgtcgagtc
     4081 gttcgccgac tggcagcggg tcatcgacat caacctgatg ggtgtcgtgc gcggctgcca
     4141 gacgttcgtg ccgctgctga aggaacaggg cgaggggcgg atcgtcaatg tcgcctcgct
     4201 cgccgggctc gtgcatgcgc cggcgatgtc gtcctacaac accgccaagg ccggggtggt
     4261 cgccctgtcg gagacgctgc ggttcgaatt ggcgcccttc gggattcgcg tcaacgtcgt
     4321 ctgcccggcg ttcttccgga ccaatctgca cacgtcactg cagggcaagg acaccgaggt
     4381 ggaggagtcg gccgtcaagc tcatcacgcg agcggggccg gacggcgacg tcattgcggc
     4441 gaaggtgatg tccggcatcg acaaggaccg caagatcatc ctcacggaca agactgggta
     4501 tgtcgcgtcg tgggccaagc gggtggcacg cccggcctat gacatcgcga tcgggtcgtc
     4561 gggcatccga ctggcgaaga gggggagcct ctgatggccg agttcgtcgc gggcgccggt
     4621 gaggtccgcg ccgaggactc gttcgatgcg gcggtggtgg cggaatggtt gcgcgacaac
     4681 gcatccgacg cgtcgggcct ggacggtgcg ccgcaggtgc agcagttcag cgggggcgcc
     4741 tccaatctca cgtatctgct gcgctggccc tctcgcgacc tgatcgtgcg tcgcccgccg
     4801 gcgggcacga aggccaaggg cgcccacgac atgggccgcg aataccgcat ccaagccgcc
     4861 ctggcgccgg tctatccgtt ggtgccgccg atggtcggct actgcgcgga cgagtccgtc
     4921 atcggcagcg agttctatgt gatggagcgg ctcgacgggc tgatcctgcg ccgcgacatc
     4981 ccggccggga tcgggctgga caccgaggcc gagcgcgggg ccttgtgcgc ggcactcgtg
     5041 ggcgccctcg cggacctgca cgatgccgac ctcggcgcga ccgggttgca ggagctggat
     5101 cgcggtccgg agtacgtcgc tcgacaggtc ggcgggtggt ccgagcgcta tagccgggcc
     5161 cggaccgagg acgtcggtga cttcgaagcg gtgatgtcct ggctgtccga gcaccagccc
     5221 gccgatcggc cgcacacgat gatccacaac gattttcgct tcgacaacct cgtcctggac
     5281 cacgccgaac cggtccgcgt cgtcggcgtt ctcgactggg agttggcgac cgtcggtgac
     5341 ccgttgatgg acgtcggctc cgccctcgcc tactgggcgc aggcggacga ccccgaggcc
     5401 atccaggcga tccggctgca gccgaccaac gccccaggga tgtggacacg cgatcaggtg
     5461 tgggcggcat acggtgcgcg gcgcgggctg gcggtgagcg agagcgagcg gcgcttctac
     5521 gaactcttcg gaaccttccg gctggccgtc atcgcccagc agatctacta ccgcttcttc
     5581 cacgggcaga ccaccaatcc caaggcgggc atgatgggcc agttcgtccg aatcctcgac
     5641 gcccgttgca cggagctgct gccatgagcc ggatcttcct cgtacgccac ggcgaagcgt
     5701 cctggcacag tgacgattac gaccggctca ccgaccgcgg acacgctcag gcgcgcgccg
     5761 tgggggtgga gctggtggcg cgtcgcgtcg tccccggcgt cgtgatgtcg gggacgctgc
     5821 gccggcatcg cgagacggcg gcggggctgt tggcaggagc gcagtggccg ctgaccgtcg
     5881 tcgaggacac ccgatggaac gaactcgacg ccgacgacat catccgggtg cacgaccccg
     5941 agcacgacac gatgaccggc gctctggcgc acttcgcggg acgcggtgcg ccgcaggatt
     6001 tcgagtcctt cttcgaccag gccatggatc gctggctcgg tggggacggc gggcacgtcg
     6061 agtccttcac ccagttcgtc gagcgggtga acgcggcact ggacgcgctc gtggccggcc
     6121 tcgcgccgca ggcaacagcg gtggtcgtca cctccggtgg cgtgcagaac gccatcgcgt
     6181 cgcagatcct cggtggtggc tccgacacgt ggcggcgcat cttcgggatg ttcccgaaca
     6241 ccggtctgat gcgcctgacc cacggcgagc gcgggttgcg cctgcgctca ctcggcgaac
     6301 tctcccatct ggagcgccag ccggacctgc tctccgaact catctattga gggagccacc
     6361 catgacgacg atcctcatca ccggcgcctc ctccggcctg ggtgccgaga tggcgcgcca
     6421 gttcgccgcc cgcgggcacg acctcgcctt ggcggcgcgc cgtctcgacc ggctcgcgga
     6481 gttgcgcgcc gagatccagg cgcggcatcc ggagcggcgg gtggagatcc gggcgctcga
     6541 tgtcacggac gacgagcagg tcttcgcggt cttccggggg ttccgcgacg acttcggcgc
     6601 catcggccgg gtcatcgtca acgccgggct cggcaagggt gccccgatcg gcaccggcag
     6661 gtatgacgcc aacaaggcca cagcgatgac gaatttcgtt gcggcactgg cgcaatgcga
     6721 ggcggcaatg gagatcttcc gcgtccagaa gcacggtcac ctggtgatga tctcgtcgat
     6781 gtccgccatg cgcgggatgc ccaagacgat cacgacgtat gccgcgacga aggccggtgt
     6841 cgccgccctc gccgagggca tccgcaacga gatggccggc gagcccgcgc tcgacatcga
     6901 cgtgtcggtg atctatcccg gctacatccg ctcggagatg aacgagcggg tcaagcacac
     6961 ccccttcatg gtcgacaccg agacgggcgt caaggccatc gtcgaggcgg tcgaggccaa
     7021 gcgcgcccac gctcgggtcc ccgcctggcc gtgggtgccc ctgggcaccg cgatgcggca
     7081 cgcgccgctg aaggtggtgc gcaagctgac ctgaggcgcc tttggcataa cctggcgacc
     7141 tcgcctttgc cttcgtgtcc ttcggcgagc ccgcacatcc cgagcgcgac gcgaatgggt
     7201 gtctggtggt gtcggcccag aggatctccg cggttgttcc cgtctgagcg ggtgtcgacc
     7261 caccgcgtca cctatgagtt cacggccttc ggcaaagtcc tcgacgtggc ccaatgcggg
     7321 ccgagcgtca ccccggagcc ggccgacatc gagtaggcac gatgaacgcg cggccgaggc
     7381 tcaggctgac gtcggccggg cgtttctgat ggctcacggt gaccacggat ccaccaagtc
     7441 gacgcccgtg tggagaaaat ccgaggtgtt ccgggtcgca cacgtggcgt gatgggcgcg
     7501 gcagatagcg gcgatctgag cgtcggccgt ggcgatcggc aggccggccc gttcgcgacc
     7561 agcgagtacg tccgcgtacg ctgcggcggc ggtctcgtcg aaggccagga tcgagttggt
     7621 gccgcgaaag gggcgcagag cctcgtcgat ggccgcactc aaagccgtct tccgtttgcc
     7681 cttcggaagg cgttgaactc ccgcgagcaa ctcggccagg gtgacagccg tgatggcgac
     7741 gtcgccgctc agagcctgca cccaggccac cacgcgcgcc tcgggctgcg acctgacgag
     7801 ctcggagatg acgttggtgt cgaggacgat cactcgaact ccacgatcac tcgaactcca
     7861 cgatcactcg aactccacgg cccgcgcgac atcggtgcgc gctggtacag gcaaatcgtc
     7921 gaccccgccg acggcttgcg cggcctccat caacgcaatc ccgatattgg gtcctcgggc
     7981 agcccgagtg aggatgtcac ggacctcggc ttccatggac cggccgtgtt cgcgggcctg
     8041 ctcggccagt tgctgcttca cggactcatc gagaccgcgc acgatgatcg acgccactcc
     8101 ccacctccca gttgatatca ccaatgatag cgttggtcct tcccgcgagc gctgagcgca
     8161 gcacccaccg agcggcgctt ccgagatctg tatgccgacg cacacgagga tctcgccgat
     8221 ggcacgggga tcaccgactc agtcgcgcct ccggaactcc acgtgaacgt gttggatagg
     8281 gtccgtgact tcaacggtga tcctcggtga cgaacccctg accatcgacc aggtcgaggc
     8341 ggtggcgcgg tcggtgccgc ccggcagcga ctatctgggc gagccgatcc acgtcgaggt
     8401 gagcgaggcg gcatggggga gggtgcggca tacccgctcc gtcgtcgaag ggctggccga
     8461 cgacacggtg gcgcactacg gcatctcgac ggggttcggc gctttggcga ccatgcacat
     8521 ccccgtcgat aagcgacatc agttgcagcg cagcctgatt cgctcccacg cggccggcgc
     8581 cggacccgag gtggagcgcg aagtcgtgcg cgcgctcatg ctgctgcgca tccagaccct
     8641 cgccaccggg cgcacgggca tccgggagga gaccctgcgc gcctacgtgg cgatgctcaa
     8701 cgcgggcatc acccccgtcg tccacgaata cggttccctc ggctgctccg gcgacctcgc
     8761 cccgctcgcc cactgcgcgc tcgcgctcat gggcgaaggc actgtccgcg acgcggccgg
     8821 cgacctgcgc tcggccgccg aggcgctcgc cgccgccggc atcgagccgg tcgacctcgc
     8881 cgagaaggag ggcctggccc tcatcaacgg caccgacggc atgctcggga tgctctgtat
     8941 ggcgatcgcg gacctgcgcg cgctcgtcac cgtcgccgac atcgcggccg ccatgagcgt
     9001 cgaaggcctg ctcggcaccg acgacgtctt cgccgccgat ctgcaggcgc tgcgcccgca
     9061 gccgggtcag gcgctcagcg ccgcgaacat gcggaaagtg ttggcggtca gcgagatccg
     9121 cgattcccac cgcgaccccg acgcctgcac ccgggtccag gacgcctact cgctgcggtg
     9181 cgcgccccag gtcgccggcg ccgtccggga caccatcgac catgcggcgc gcgtcgccgg
     9241 ctgggagctg gcgagtgccg tcgacaaccc cgtcgtcacc ctcgacgacc gagtcgagtc
     9301 caatggcaac ttccacggag ccccagtcgc gtatgtcctg gacttcctcg ccatcgccgc
     9361 cgcggacttg gcgagcatga gcgaacggcg caccgaccgc ttcctcgaca aggcccgaaa
     9421 ccacggcctg catgccttcc tcgccgacga ccccggcgtc gactccggcc tgatgatcgc
     9481 ccaatacacc caggccgcaa tggtttccga gatgaagcgc ctcgccgcgc cggcgagcgt
     9541 cgactccatc cccagcagcg ccatgcagga ggatcatgtc tcgatgggct ggggcgcggc
     9601 ccgcaagctg cgccgctccg tcgatgccct gacccacgtg ctgacgatcg aactgctcgc
     9661 cgccgcccgc ggcatacagc tacgcgcacc gctgcggccc tccccggtaa gcgcggcggt
     9721 catcgccgcg ctcgacgagg tcacgcggac ggctcctggg cccgaccgca tactggccga
     9781 cgacatcgcg gaagccgtgt atgccgtggc cgacggctcg ctcctcgctg ccgcggaggc
     9841 agccgccggt ccgctgaact aggcggcggt cggcgccggc ctacagcgct gcttcgcctg
     9901 cgggcgtcat cagcccgcga gtgcgcagct ccacgacgag ccggcggttg cgctcggagg
     9961 tcgttccgcg agtgcgtcgt ggggtgaagc gctgcaggta tgacgtgtcg tcgcccgccc
    10021 gtcgcgtcga ttcgctccag ccgaagcaga gcccctcctc gagcaggtcg tggaaggtca
    10081 cgctgggtat gccgctgccg gccttcgcca cgcgcaccca aactcctgag gagcggctgt
    10141 gctcgcgctc cagccacgac cgcaactcct ggcgcgtcgc gaacgactgg accatcagtc
    10201 catcgggcgg cacggacgag tccgcgtcgc cgatagcccg ggtcctcctc tgtgccatgg
    10261 gtcgtcacct cctctcgatg ctggttgggc ccggtcagcg gatgaggcag aaggggtggc
    10321 cggcgggatc gaggaagacc ttgaagttgc cgttctccgc cggctggtgt tcgtggaccg
    10381 tggcgccggc cgcgaggacg gcaggttcga cggcctcgat ctgacccgag cacgacaggt
    10441 cgaggtggaa ctgctgcggg tgggcgccgc cgggccatgt gggggcgaca aagtcgggga
    10501 tctgttggaa ccccagacag gttcgcccgt tcggattttc cgtcgagatg ccaccttccg
    10561 gcggggcaat gacgacgaag tcggcgtccg ggtcgccctc gggctgccag ccgaggatct
    10621 gcgcgtagaa gcgtgccaga gccggagcgt cggggcagtc gaggatgacg aggtcgagtt
    10681 tcgtcgtgat cgtgctggtc atggagccga cggtatgccg cgtagcggac gaaaagtgcc
    10741 cgtgtcctcc cactcgatgt gcgttacgtg ttcactcgac gggagatacg tgttcactcg
    10801 acggggggtg cgcggtggtc acccgactgg gggcggggtg agaggatgcc agcggcatac
    10861 gactcgacga tgaggagatc cggatggacg gggcacgacc agttcgcgcg gcgcggggga
    10921 cgcagctgac cgcgcggcag tggagcaccg aagcccccct gcgcatgctc atgaacaacc
    10981 tcgaccccga ggtggccgag cggcccgacg acctcgtcgt ctatggcggc accggccgcg
    11041 cggcgcgcga ctgggcgagc ttcgacgcca tggtgcgcac cctgaccacc ctcggacccg
    11101 acgagaccat gctcgtccag tccggcaggc ccgtcggcgt cttccgcacc cacgagtggg
    11161 cgccgcgggt cctcatcgcc aactccaacc tcgtcccgga ctgggccaac tggcccgagt
    11221 tccgccgcct ggaacacctc ggcctgacca tgtacgggca gatgaccgcc ggctcgtgga
    11281 tctacatcgg cacccagggc atcgtgcagg gcacctacga gaccttcgcc gcgatcgctg
    11341 agaagcggta tggcggcacg ctcgccggca ccttgaccct caccggaggc tgtggcggga
    11401 tgggcggggc gcagccgctg gccgtcacgc tcaacgaggg cgcctgcctg atcgtcgacg
    11461 tcgacccgca acggctgcag cggcgcgtcg agcaccgcta cctcgacgaa gtcgccgacg
    11521 atctcgacga cgctatcgaa aaagcgttgc gcgccaaggc agaacgccgc ggccactcgg
    11581 tgggcgtggt cggcaatgcc gccgaagtct tccccgaact gctgcgccgc ggcgtcgaca
    11641 tcgacatcgt caccgaccag accagcgctc acgacccgct gtcctatcta cccatcggca
    11701 tcgacctcga tcagtgggac gaatacgcga cgaagaagcc tgaggagttc accgaccggg
    11761 cccgcgagtc catggcccgg cacgtcgagg cgatggtcgg cttcctggac ggcggcgcgg
    11821 agaccttcga ctacggcaac agcatccgcg atgaggcgcg gctcggcggc tatgaccggg
    11881 ccttcgcctt ccccggcttc gtgccggcat acatccggcc gctcttctgc gagggcaagg
    11941 gcccgttccg gtgggccgca ctctccggcg acccgcagga catcgccgcc accgacaagg
    12001 ccgtcctcga cctcttcccc gacaacgacc ggctccacaa gtggatccgc ggggcgcagg
    12061 aacgcattgc gtggcaagga cttccggccc ggatctgctg gctcggctat ggcgagcgcg
    12121 acaaggccgg gctggccttc aacgacctcg tcgcctcggg cgccgtcagc gcgcccatcg
    12181 tcatcggccg cgaccacctc gactgcggct cggtcgccag tccctatcgc gagaccgagg
    12241 cgatggccga cggctccgac gccatcgccg actggccgct gctgaacgcg ctcacggcga
    12301 cgagttccgg ggcgacgtgg gtctcgatcc accacggcgg cggcgtcggc atcgggcgct
    12361 ccatccacgc cggccaggtc agcctcgccg acggcacccc gcttgcggcg cagaagctcg
    12421 cccgggtgct gaccaatgac ccgggcatgg gcgtcatccg ccacgtcgac gccggctacg
    12481 agcgggccga cgaggtcgcg gccgagcggg gcgtgcgggt ccccatgcgg gaggcgtgan
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cggcgaggca ctcgcctggg
    12661 ccgtccggcc tggtcaggtg accgggtcga aggtcaggac gccctggctg gcacaccggc
    12721 cggggacgtg attgcatgca ggcatgtcac cgtcgacgtt ccaggccatg tgggctgccc
    12781 tcagccccgt cggccgggac gcgcagaccg ggggatatcg acgtttcgcc tggtcacccg
    12841 aggaccacca gctgcgggaa tggttcgccc aggaggcgac cgcccgcggg ttggagctga
    12901 ccgaggaccg tatgggcaac cagtgggcct ggtggggcga ccccgacaca gcggggccgg
    12961 gtctggtgct cggcagtcac ctcgactcgg tgcccgatgg cggggcgtat gacggcccgc
    13021 tcggcgtcgt ctcagcgctc gccgccgtcg atgcgctccg gggtgccggg cataggccga
    13081 accgcccgat cggcgtcgcc aacttcgtcg acgaggaggg cgcccgcttc ggcatcgccc
    13141 tcgccggctc ccgggtgatc accggactgc tcgacgccga ccgggcccgc tccctgaccg
    13201 acggcgacgg catcacgatg gccgaggcca tgcgggccgc gggccgcgac cccgacgcga
    13261 tcggccccga ccccgagacc ctgcggcgcg tcgggaccta tgtcgaactg cacgtcgagc
    13321 agggccgcgc cctggccgac ctcgacgcgc ccgtggctgt gggcaccgac atctggcccc
    13381 acggccgctg gcggatcgac gtgcccggcg aggccaacca cgcgggcacg acgcggctgg
    13441 cggatcgccg cgatgccgtc ctcggcctgg ccggtgtcat ccagggcact cgcgaggcgg
    13501 ccgagcggcg cggctgcctg gcgaccgtcg gcaaggtcgc gatcgaaccc ggcggcgtca
    13561 acgcgatcgc cagccacgcc accggctggt tggacgcgcg aggtgccaac ccggaggccg
    13621 tccgcggcgt catacaagac gtgacagcga tggcgcgggc ccacggcggc gcggttcacg
    13681 aggaatcctg gaccccggtc accgagttca cccccgactt ggtaagccgg atgcgggagg
    13741 tgctgggcgg cataccggcc ctcggcaccg gggccggcca cgatgccgga gtgcttgcgc
    13801 aatacggcat cgagacggca atgctgttcg tgcgcaatcc gaccggcgtc tcgcactcgc
    13861 cggccgagtt cgccgaggag gccgactgcc tcgccggggt gaaggcactg gccaccgtgg
    13921 cggcggatct gacgggggga gaacgatgac gaccatctgg gcgcgtcacg cgcaactgcc
    13981 cgacggcatg gcggcgaagg tccgcatcac catcgagggc gaccgcattc gcgcggtcgg
    14041 gcgcggcgag ccgcaacccg acgacatcag cctcccgctc gtgctgccgg gcatcgccaa
    14101 cggccactcc cacgcctttc accgcgccct acgcggccgc gtgcaggacg cggcaggcaa
    14161 cttctggacg tggcgcgatc acatgtatgc cgccgcgtct gccttgaccc cggagagcta
    14221 tttcgctctg gcacatgggg ttttcgtcga gatggtcacg gcagggttca ccgccgtggg
    14281 ggagttccac tacctccacc atggagccgg cgggcgtccc tatgaaaacc cgaacgcgat
    14341 gggcgaggcc gtgatcgcgg cggcgcaggc tgccggcatc cggctcaccc tgctcgacac
    14401 tctctatctc gcgggcggtc tcggcggcga ggggcacctg ccgctcgacc ctgtccaaga
    14461 acgcttcgcg gacaaggaca ttgccacgtg ggcgcgccgg ttcggcgacc ttcgtgacac
    14521 cgcgggctgc cggatcgggg tcgccgcgca ctcggtgcgc gcggtgcccc gcgaggcgct
    14581 gctccagctg gcctcggtcg cggggccccg cgtcatccat gcccacgtca gcgaacagcc
    14641 cgccgagaac gccgcgtgca ccctggtcta cggccagacg ccgacgcaac tgctggacaa
    14701 ctccggcctc ctcagcgaac gcttcaccgc cgtgcacgcc acccacctct cacgtgacga
    14761 catccggcgc atgggccgcg cccgggcctt catctgcatg tgcccgacga ccgagcgcga
    14821 cctcgccgac ggcatcgggc ctgcccgtga cctcctcgac gcaggcgccc ggctgagcat
    14881 cggcaccgat cagcacgtct cgatcgaccc gttcatcgag gtgcgcggcc tggagatgca
    14941 cgagcgcctg cacagcggcg aacgcgggcg tttcactctc gacgaactca atctcgcgat
    15001 gagccaccac ggttacgcga gcatcggctg gcccgacggc ggtctcatcg cccccggtgc
    15061 gctcgcggat ctcgttgctg tgcgccgtga ttcggtgcgc acggtcggat cccgcgccgc
    15121 gcaggtcatg ttctcggccg ccgccggcga cgtcaccgac gtcatgatcg gtggcgagtt
    15181 cgtcgtccgt ggcggtgtcc atcgcgcagg cgatccgggc gcgctgctcg gcaaggcgct
    15241 gatgaagctg cgcggggcat gagcaggccc ctatgacgag cgcgctcgtc accgggatcg
    15301 gccaactcgt cacgtgcgct ggaccgggca gcactcccga ggacgccctc gggatccgca
    15361 ccaatgcggc tctcgtggtc gaggacgacc gcatcgcctg gatcggcgcc gccgccgacg
    15421 caccgcccgc cgaccgccgg atcgactgcg aggggcgggc ggtcctgccc ggcttcgtcg
    15481 acagccacac ccacctggtg tatgccggcg accgggcggc cgagttcgag gcgcggatgg
    15541 cggggcggcc gtatgacggc ggcggcatcg ccacgaccgt caccgccacc cgcgcggccg
    15601 gcgacgacga gctgcgggag ttgctgagca gccggatgac agagctgcgc aggcagggca
    15661 gcaccaccgt cgagatcaag tccggctacg gcctcgacgt cgacggcgag gcgcgcctgc
    15721 tccgcctggc tcgcgaggtg acgaccgaga cgacctacct cggcgcccac gtcgtgccac
    15781 cggaatacgc gggtgaccgc gcggcatacg tcgaactggt ttgtggggca atgctttcgg
    15841 cgtgcgcgcc gcacgcgcgg tggatcgatg tcttctgcga acccaactca gcgcacgcct
    15901 tcgacggcga cgaagcgcgg accgtcctga cagccggcat ggccgccggc ctgctcccgc
    15961 gcgtccacgg caaccagctc ggccccggcc ccggtgtgca gctggccgtc gaactcgacg
    16021 ccgcgagcgt cgaccactgc acttatctca gcgacgccga cgtcgacgcg ttggcgggtg
    16081 cgccccagac cgtcgccacg ctgctgcccg gcgtcgagtt ctcgacccgc tcgccctatc
    16141 ccgacgcccg ccggctgctc gatgccggca tcagcgtggc cctggccagc gactgcaacc
    16201 ccggcacgtg caactcctcg tcgatgccgt tcatgatcgc cctcgcggtg cgcgagatgc
    16261 gcatgactcc tgctgaggcc gtgctcggcg cgacgcgcac ggcggcgaaa gcattgcggc
    16321 gcaacgatat cggagccatc accgtgggga tgcgggccga cctgacggtg ctggacgcgc
    16381 cctcctatgc acacctggcg tatcgccccg gcgtccccat cgctcgcgcc gtcgatctcg
    16441 cctgagtgct caggcctgcg cctcttcttc cttcttgcgc atcggctcgg gcaggtgtcc
    16501 ggcggagttc gcgtagtact gagcggcctt gcgggtgaag aaggtcttgg cgacggccgc
    16561 tgccaatgcg ctcacggcgg cgagggccaa cgcctctccg aggggcgtgt tggggttttg
    16621 tgggtcgacg ttctcgtctt tgcccgcctt gctccagatc gccgtcaccg acttgttcgc
    16681 gacgagaccc gcgacgaccg ccgcgcccgc cgcaagtgct ttccagacca ttccgcccat
    16741 ggccacacgt tagcctgtcc atcatgcggt ggcggcggac agcccttgcc ttgttcatcg
    16801 gcgcggccgc gtccggactg ggcatcctcg ccggctccaa catctgggtc gcccgggcga
    16861 gcgcgggccg gatgtatgcc gtggcggacg tcccggccaa gccggtcgcc ctcgtcctgg
    16921 gtgccggtct gacgccggcc ggcgagccca cgccatacct cgccttccgg ctcgacctgg
    16981 ccaagcagct ctacgacgcc cggaaggtcg aggtcctcct cgtctcggga gacaaccgca
    17041 cccacgccta cgacgagccc acggcgatga tggactacct cgtcgctcag ggggtgccgg
    17101 caacccggat cgtgcgggac ttcgccggcc gcgacaccta tgactcgtgc gcgcgggcca
    17161 cgcgcatctt cggtgtcacc gaggcgatcg tcgtcacgca ggaataccac ctgccgcgcg
    17221 cggtcgcgac ctgtcgcgcg gtcggtgtcg acgcggtcgg cgtcggtgac acccgcggcc
    17281 agcagtatgg cgccacctgg agtcagggcc gcctgcgcga gcgggcggcg gtggtcaaag
    17341 cgagctggga catcagtatt cggcgcgacc ccgtcctcgg cgcccaggag agcggcgtgc
    17401 gcgatgctct gagcaccctt cgctgacgga tcagcgggcc ccgccgacgc gcggcagcga
    17461 ggcaggcgcg cggcatacgg cccggtcgat ggcgcgcacg cgcaggacta aggtttggtt
    17521 catgagtcgt tccagcgccg tttcggctgt ccccgttccc agttctcctg cccagatccg
    17581 caatgtcgtc ctcgtcgggc cgagcggcgg cggcaagagc acactgttcg accggttggt
    17641 cagcggggaa tcggcgttgc ggcgcaagga agacgactcc gcatcgacga cattgcgggt
    17701 cgcgaccgtc ggggtcgacg accttgccgt tacccttctc gacaccccgg gtgcgccgga
    17761 cttcgtcggc gaagtgcgcg cggggttgcg ggccgcggac gccgcgctgt tcgtcgtcgc
    17821 tgcgggcgcg tcggtcgacg aggcctcccg gatgttgtgg cgcgagtgcg ccgcggtggg
    17881 tatgccgcgc gccgtggccc tgacgaagct cgagcagtgc caggacgact acgcgacgat
    17941 cgtcgaggcg tgtcgtcgcg cgttcggcga tgcgcagccg ttgcaggtgc cgattctcga
    18001 aggggacaag gtcaccggtc tgctcgacct gctcggccag agcgttcacg gccccgacgg
    18061 cgaggtgtcc ggcaccccca cgggcgacca ggcggcgatc atcgaggacc tgcgcggcgg
    18121 agtcgtcgag gcgatcatcg aggagtccga ggacgagacg gccctcgaca gctatctcaa
    18181 tggcgacgaa ctcgacgccg acacgctgac caaggacctc aaggccgcca tcgcgacggc
    18241 ccggttcttc ccgatcatcc cgacccactg ggccagcggt gtcggggtcg gggaactcct
    18301 cgacctcttc gccaccggtt tcccctcacc ggcggatgcc tggctgcccg aggtcaccac
    18361 tcccgacgcc ggcgagttcg gcgagatcga gtgcgacccc gccggcccgc tcgtggccga
    18421 ggtcgtccgg accagcagcg accagtatgt cggccgcctc tccctcgtcc gcatcttctc
    18481 cggcaccctc accccggacc agcaggtcca cgtctccggc cacctcgagc acctcaccgg
    18541 ggaggaggtc gaaggccacc ccggccacga cgacaccgac aagatcggcg gggtgcagac
    18601 cccgaccgcc gtcgacccga ttgtccgcac ctccgccatt gccggcgagg tcgtcctgct
    18661 gaccaagctc gcgcacgcgg agacctccga caccatctcc gccaccgaca agccgggcct
    18721 cgtccagccg tggctgctgc ccgcgcccct gctgcccgtc gccattcatg cggcgtccaa
    18781 gaacgacgag gacaagctcg ccaccgccct gcagcgcctc gtcgccgagg acgtcacgat
    18841 gcggctggaa cacaacgtcg agacccacca actcgtgctg tgggcgctcg gcccggcgca
    18901 cgtggacgac ctgctcgcgc gcctgaagga ccgctaccac gtcaacgtcg aggtcgagcc
    18961 gctcaagacg tcgctgcgcg aaaccttcgt gcgcgaatgc gacctgcagg gccggctggt
    19021 caagcagtcc ggtggccacg ggcagtatgc cgtcgtcaag gtcaagatcg agccgctcga
    19081 gcggggcgcc ggcatcgagt tcgtcgacaa ggtggtcggg ggtgccgtgc cgcgccagtt
    19141 catcccctcg gtcgagaagg gcgcccgcaa ccagctcgag aagggtgtcc tggccggcta
    19201 tccggtcgtc gacgtccgcg tcacgctcta tgacggcaag gcccactcgg tcgactcctc
    19261 cgacatggct ttccagaccg ccgcggccac cgcgctgcgt gaggccgcca acgagtccac
    19321 cgtctcgatg ctcgagccgg tcgaccagat cgtcgtgacc gtcgccgacg acaacgtcgg
    19381 cgccgtcatg gccgacctgc gcggtcgccg cggccaggtg cacggcaccg agcccgccga
    19441 caccgagggc tttaccaagg tgacggccga ggtgccgcag cacgaactct cgcgctatcc
    19501 gatcgacctg cgctccgtct cgcacggcac ggggtccttc acccgcacct tcgtccgcta
    19561 cgactacatg cccgcagccc aggccaagga ggtcatcgcc aacgcctgaa accccttgcc
    19621 ccgcaaggaa cttctgctag gcccgccacc ctggcgtcag ggtggcggtg gtctgcccgc
    19681 cttgcgggcg ccggcgcgtt cgctgcggcg gatgagttgg cgcagttgtg agcgcgaaag
    19741 cacaactccc gcatcgggat ccgccgccgg gagcgaccag ccgaggcgtc gcgcgagcaa
    19801 ggtgagcccg ccgccgatga cgatgccggc cgccataccc cacgtcagcc gcgactgcac
    19861 cggcaccaac aacatcgccc ccgacgccgc agcggccgcc gtcgcataca gcgtgttgcc
    19921 gccgagcacc gcaggcacct gcccgatgca gatgtcccgc accatcccgc cgccgacggc
    19981 ggtgacgacg ccgagcagga gcgagggtag ccaaccgagt cccaccacct gcgccttgaa
    20041 cgtccccgtc gccgcccagc atccgagggc cagggcatcg gcgatcagga gcggccagcg
    20101 ccagcggcgc ccctcgatcc ggaagacgag cgcgatcagc acccccacga tcgccgtcac
    20161 caggtaggcg ggatccgtca gggccaccgg ctggccgacg ttgagcatga tgtcgcgcag
    20221 cgcgccccct gccaggccgc tgacgatgcc gagcacgatc atgccgacga ggtcgaagcg
    20281 ccgaccacgg gcgatggtcg cgccgatcat gccgttgcac agcacgccgg cgaggtcgac
    20341 gaaccggaac gcctcggaga catcgagcgc tgtggacacg tgcgccatta gagcaggcaa
    20401 gggctcactc cgccgcgtag gccgtgacct cggtcgcctt ggcgctcagc cacacggcgc
    20461 ttcccggcgt caggcccagg tcgagaatcg ctgccggtgt cacgtcgacg agggcgtcgg
    20521 gtgggccggc gacgtcgagg cgagcccgat cggcgatctg ctgtatgccg cgcacccgcc
    20581 ccgcccacac gttgcgggcc gatccgtggt cgggccgggt cgtgtggaca gtgatcgcgc
    20641 tgggcgccaa tgcaatcagt acctctcgcg accccgatga cgattgcgcc gggaggtcca
    20701 ccgccaccag tcgtccgcca ccgtccagct cgatcccgcc ccgcgatacg cgtccccgat
    20761 agtggttgag ccccgccaac cgggccacgt atgccgtcgc cggacgccgc gcgatcgtcg
    20821 ccgggtcggc gtcctgcgtg acgcgtcccg cctcgatgac cacgatgcgg ccggcgagga
    20881 ggagggcgtc gagcgggtcg tgcgtgacaa gcaatgccgg ccctgcgaag gcgctgacga
    20941 aagcacgcaa ttcggcgcgg gtggtgtccc gcctctgggc gtcgagggcg gcgagaggtt
    21001 cgtcgaggag caacaggtca ggtttgctgg ccagggcgcg cacgagcgcg acgcgctggg
    21061 cctgccctcc gctgatccgc gccggccgcc ggtcggcgat ctcggtgagt ccgaagtgcg
    21121 ccaactgcgc gtccgcgttc gcgagggccg cgccgtgccg cataccgcgg gctcgtgccg
    21181 ggaaggcgac attctcgcgg acgctcatcg tcgggaagag tcggtggtcc tggaagacga
    21241 cgccgagccg gcgctgctcc ggaacgaccc aggtgcccag cgctgggtcg tccagggtcc
    21301 ggtcgccaag ccgaatccgc ccgccggaca acggggtcag cccggcgatg gcccgcagca
    21361 gcgtcgtctt cccggcgccg ttcgggccga gcacggcgac gacttcacca gggccggcgg
    21421 ccacgtgggc ctgcagcgcg aacggccgcg ccccctgatg gaccacctcc acccgggcgt
    21481 ccagggcaac agggtcggcg gcgtccgaag gagcggcccc catcacgccg ccgacccggt
    21541 cgtcagccag cgtccccgta gcagggccag caccacgacg catacgacga gcatgaccag
    21601 cgacagggcg cttgccgcgt ccgggtcgcg ctccatcgcg agatagaccg cgagcggcat
    21661 ggtttgggtg cggccggggg agttgccggc gaaggtgatc gtcgccccga actccccgag
    21721 cgcccgcgcc caggccagca ccgtcccggc gagcagggcc ggggccatca tgggcagcac
    21781 gacccgacgg aaggcgaacc acgggctggc gcccagagtg gcggcggcct cgccatatcc
    21841 gtcgtcgagg gtgcggaagg cgccttcgac ggtcagcacg aggaagggga gcgcgacgaa
    21901 ggtctcggcg acgatgacgc cgaagggggt gaacggcagg ctgaatccga agaccacgtc
    21961 gatgggcttc ccgagcagtc cgcggcggcc gaaggcggac agcagcgcca cgccgcccac
    22021 caccgggggc aggaccaacg ggaccgtcac gagcgcccgc acgagcccgg tcaggcgtcc
    22081 ggtggatcgc gccagcagcc acgccagcgg tatgccggtc acggcagcca gcaacgccgc
    22141 gatcgtcgcg cagagcaggg agagccgaag tgcttctccg acaatgggtt cgcggaacag
    22201 gtccggcagg tgcaaccacg gtacgcgaaa cagcagcgcg accaacggca gcacgaggaa
    22261 cgccgcagct acggccgccg gcactcgcag ctgccacggc gtcctcatcg ccgccccagt
    22321 cgagggcaca ctccggggct gtcgagagcc acatccttga tcatcggggc acttcggtta
    22381 gtcgagggtc atggcgcggg cgcgaagccg gctgccttca gcgcatcctg cccgatcgtg
    22441 ccggtgacca ggtcgacgaa ctgctgagcc agcgtcgggt tggcgctctc cgtggtgacc
    22501 gcgatcgggt agtccgtcgt tgcactcacg tcagcaggca gcgtgatctg ggtgaccttg
    22561 tcgcgcgcga gcgcggcatc ggtgacatag acgagcccgg catcgacctc gccgaggctg
    22621 accttggtca gcacggccgc cacgtcgacc tcctcgctga ccggtgtgac gtccagatct
    22681 gcctgttcga aaaccgtgag cgccgccgct ccacacggaa cgtccttggc gcacaccgca
    22741 accgtcgtgt ccttgctcgc gagatcgctg acatcgtcca ccgtgccggg attgtcctta
    22801 ggcaccacga tcgtcatcga gttggttgcg aaggtgaccg gggcgttcac ggcgccggca
    22861 tcttcggctg tcttcatcgt cttcgcgctg gcgctcgcga agacatccgc gggagcacct
    22921 tcgacgatct gtgtcgcgag accggaggac gggccaaagc tgaactccac cgtggacccg
    22981 gggtggtctg cctcgaactg gtcgcccagc ttggcgaaaa cgtcacgtag cgaggccgcg
    23041 gcgaggacgg tgaggctccc gctggccacc ggtcccgagc cggacccgga ggccgatccg
    23101 gcgccagtcg agcccgcgct cgacgatccg gacgaggagt cgccgcagcc ggcgagagcc
    23161 acggccgcga gaatcacgac gggggtcaag cgggcgagag cacgcatggt cagggggcct
    23221 ccggcagttc gatgacgacg tttgtggact tgatggacgc gaccgccagc acacctggct
    23281 cgagtccgag gtcgtctgca gcctcgcgac tcatcaacga cacgacccga tgtggccccg
    23341 actggatttc gacctgcgcc atcacggtgt ccttgacaac ccgggtgacg agaccgacga
    23401 agcggttgcg ggccgactcc gagaccaccg gccggggctg cggccggggc atctcggccg
    23461 ccagcgcccg ggcataggcg gccagttcgg cgccgtcgac ggtctgccgg ccggaggcgt
    23521 ccaggctcgc ccgcagccgc ccttggtcga cccaccgccg gacggtgtcg tcggacaccc
    23581 caagtaggtc ggcagcctcc ttgaaccgaa aatgcggcat ggaaatgagg atacacacgc
    23641 agacacggat cggttggacg ggtgggggcg cacgcgcaat agggtctggt catggccacg
    23701 gtgtcctttc ccagcaaggg ctgggtccgt gacgtgggac ccatcgaggg aatcaccccc
    23761 gtcgtgtggc acccggacaa cccaccgccc gacggcccct tggatgtcgt cgtcgccccc
    23821 tatgtcggtg ccgccgcgtg catcgcctcg gtggacaagg tccccggtgt gcgcctggtc
    23881 caactcctca ccgccggctt cgacggcatc ttcgaacgtg tgccggatcg catcgcaatc
    23941 gcgaatgccg ctggcgtgca cgacgattcg acggccgagc tggcgctggc catgattctg
    24001 gcgtcgcagc gtggcatccc agacttcgtc cgggcgcagg agcggggggt atggctgcgg
    24061 cccggcttcc tgccctcgct cgccgaccgg cgggtgctgg tcctcggcta tggcggcgtc
    24121 ggccgggcca tcgtcgagcg gctcctcccc ttccgggcac gcgtgagcgt ggtcgcgacg
    24181 acggcacgcg acggcgacga cctggtcaag cgtgtgcggg gcatggacga actcgaccgg
    24241 ctgctcaaga agtccgaagt cgtcatcctc gccgtgcccc tcaacgacac cacccgcggc
    24301 ctggcagacg acgcgttcct gtcgaaactt cccgacaacg ccctcgtcgt caacatcgcc
    24361 cgcggcccgg tcctcgatac ggatgccgct ctgcaccata cggatcggtt gcgtttcgct
    24421 ctcgacgtca ccgacccgga gccgctgccc ccggagcacc cgctctggca ggcgcccggg
    24481 gtcctgatca ccccgcacgt cggcggcccg tccacggcct tccgcccccg agccgtggaa
    24541 atgctgcgcg agcagctcgg ccgcatcggc cgcggtgagg cgccggacca tatcgtgcgc
    24601 cccgcagccc cctagacagc ggccgcccaa gctgactcag cagccccgtg tgcaccgagc
    24661 ggggcgactt agcagaggtt ggtgaaatca gcacacctag ctaggtctgc tgatttcacc
    24721 aacctttgct agcagaagtt ccttgggggc aacgcagttg cccggcctca gcaggccatc
    24781 gtcggggcgt aggcgatgct gcccttggcg gagagggtgg ccaggtcggc gccggcgagg
    24841 ccgagcattg cctgggccgt ggcgcgcggt gccgcggcag cgtcgaaggg ccgggtgtag
    24901 gcgatcgcgg cgcggcggct gaagggggcc gcacacccga gttggttgga gcgcaccacc
    24961 cgccgggccg tgtccgccgc cgcgccgtat gccgtgcggt ccaggcccgc cgccaccagg
    25021 ccggccgtgt tggagttcgt caccccggga gacttcacat actgcgcgag ccacgctccc
    25081 gcgcgcgcgg ccgcgccgct gcgcccggtc gcggcgaacg cctggatcgc cagggcggtc
    25141 gcgtccgggt cgccggcgca cgggctctgg tcgaaggcga tcggaacgct gccgtcggcg
    25201 caggactgcc gctgcaggta acccgccgcc cacccggccc gacccgacag cccggcccgc
    25261 tgcagcgcga gcacgccgaa gctctgccca aaggcgttcg aatagtcacc ccactcagac
    25321 ttgtccgaca accgccccga actcgtttgc agcgcagcga gttccgcgat gatgttgcgc
    25381 cctccgaagg agcggacatc gcgcccggtc acgtcgatgg cgagggccag cttggcgtgc
    25441 gcgccggcat acgactcgcc gccggttccg acataggcac ccagggtggc gggctgggcg
    25501 atccaggaca tcgcccggtc actggtgacc ttgttggtct tcgccgcggc gaaggccagg
    25561 acggcatcga gcgtcagacc cgggtcgtcg tatgccgtgc cgccgaagtc ggtctgcagg
    25621 tgcgtgccgt cgacgaactg ccggttcagc cagccggcgg cggtgcggcc ggccagccgg
    25681 tcggacggag tgggtgccgc agcaaccggc ccggccgcca cggccagcgt ggccgcagcg
    25741 gcgacgacgg tcacgccggc gcggcgaacg cccttgcggg acagggaaag aggggacaga
    25801 gcaagagagg ggacagacac gagaggtcct tcctccgcat acctcggcgg ggagtcgatg
    25861 tgaatggaca tgtccgtcac aggcattcgg gctcatccgg ccagaggccg acatcaccgc
    25921 tgcgggtcag cgccggattc gcaccggctt cccccgcgag ggacaccccg aacctaccgc
    25981 acggtctcag cggcggcgca gccagtcccg gaccgtgtcg atgatggtgt cggcgacctc
    26041 cgtgacgatg ctcgacgccg gcgggccgtc gggggagttg gcggccgact ccacgcccgg
    26101 cgcgccctcc gacagcacga cgatcgacca cggcgcggcg gcatagtcgc ccacgatgac
    26161 gtcggacttc tccagcgccc agcagttgta gccggcctcg aagtgcgagg cggtgtcgac
    26221 gatccggtgc cacgccaccc cctcggcggc cgcgggcacc ccgaagcgca ccgcctggtc
    26281 ggccatattg atcaacacca acagatcgga tccgccgacc ggcgaggcgt cgatccacag
    26341 ccgcaccgcc cggtcaccgt ccgtcggcgc gtccgccgac tgcggctggg tgaagacata
    26401 gctcacgtcg gtgccgccga gttcggcatc gccgtacgac cgctgccgca accccgggtg
    26461 cgagcgccgc agcgccgtca gatacgtcgt gaacaccaag tgcggattga ccccggcggg
    26521 cgcggccgcg accccgaagg tgtcgtggta ggccgcgccc tcgaccccga ccgcagcctg
    26581 cgtcggcgcg ttggtcgctg cggcggccca gttgttccac atcccgatcg tgttgaggtt
    26641 gtagggattg ttgttgccgt tctgggtccg ggcgaactcg tcgccggagc acatcatcgg
    26701 cacgccacgg gccaggatca gcgcgaccat gaagttgcgc aggcgctgcc gcacgaaggt
    26761 ctggttgccg ccgctgcccc acgagtcgtt gctgtcactg ccgccgtcgg acggcccgaa
    26821 cggccacggc tggccgttga ctttgccgtc ataggacacc aggtcgagca ggcagaaacc
    26881 gtcgtgcgcg gtgacgaaat tgatcgaccg ctgcggcccg ccctggtcgc ggaagccctg
    26941 atagtcgccg ttcatcatcg ccatgaacgt gccggtgttg ccgtccccct tcaggtagcg
    27001 ccgcacggca tcgcgatagc ggccgttcca ctcaccccag ccgtcgggga agttgccgac
    27061 ctcgtatccc cacaggtccc acgcctcggc gatgacctcg atgtcgcggg ccgccgccag
    27121 gtcgcggatc gccgtcagca tcgggtgctc ggagtggaag cgccgctgct ccccccatgc
    27181 ctcccgcggc gccgcgtcgg gcagccggcc gagcacgggc gccaggtcga accggaaccc
    27241 gtcgacgccc atctcgtcac accagtacgc cagcgattcg agcacgaggc gctgcgccac
    27301 gggcgaggag aggtcggtct ggttgccgca gccggtcgcg ccgtcgacga gcagcccggt
    27361 gtcggtgagc acgtagtagt cgggcgtcgc gaatccgccg aaactcgtga acccgaccgt
    27421 gtcgccgtcg ccccagttgc cgccctcgcc ggtgtggttg tagacgacgt cgagatagac
    27481 ctcgatgccg gcgtcgtgga aggcgcggac catggccttg aactcccgcg tcggcccgcc
    27541 gggggatttg tcggcggcat actgccggtt cggcgcgaag aagccgagcg tcatatagcc
    27601 ccagtggttc gaggatccgg cggtgtcgag ttcgctgccg ctggtctcgt gcaccggcag
    27661 cagttccagc gtcgtgacgc cgagggcctt gaggtagggc gcgagcagcg ccgcaccggc
    27721 atacgtgccc tgcaggtgcg ccgggacgtc gcccacgccg gcgaagcgcg gcaggccgga
    27781 gagcagggta tgcagctgcg tcgccgaggg gtgcgaggtc aggttgcgca cgtgcgcctc
    27841 gtagatgatg gcgtcctgcg ccgccacctt cggcttggtg ccggtgggcg tcgcgtcggc
    27901 gaccacgatg gccttcggag cgtatgccgc ggtttcccac tcccgcagcg gccgcccgcg
    27961 gtagtcgcgc gggccccagc cgaagatccc cgcgtccgcg cccgccgccg ccacctccgg
    28021 gctcgccggg ctgtgggaga tctcgcgggc gtacgggtcg gtcagcagct tgttggggtt
    28081 gaaccgctcg ccgcccggtc cgatgtccgc gacgaaacca gcggccgaac cgggggtcca
    28141 ggccgggtcg acggtccagc ccggccccca cacgcgatag ccgtagtgcg cgcccggccc
    28201 gaccccggtc agccgcgccc gccacgtccc gtcggggccg cgctgcgcgt cgaccacggc
    28261 caccggaccc gcgccggtgg cgcgcgcata gatctccaac tgcacgcgcg tcgccgcggg
    28321 agcggtcacg gcgaaggtcg tgctcgagga gttcgcgtcg aaatgcgcgc cgagcggcca
    28381 ggtggcggcg gcccaggagg gcgcatcgac gggggcgggc agggcggcga tgggcgcagc
    28441 ggtggtggtc acggaaggag cttcccggga gtcggagcaa ggtcgaggtg cgtcccctca
    28501 gcggcaggcg gcacaactgc ccgagagggc ggtatgcgtg gggtcgagct cgaagccggc
    28561 ccgtgcgaca taggcggcca cgggatcgag gatctcggcc ggcaggtcga ggacggcccc
    28621 gcaactgcgg cactgaaggt gcacgtgggc gtcgtcgccg tcggtcaaat gataagcggt
    28681 cgcgccgtgc ccgaggtgaa tgtgctggac cacgcccacc ccggagaggg cctcgagcgc
    28741 ccggtagacg ctgctcgccg gcaggtcggc gccagcccgg gcgtggatgt cgtcggccgt
    28801 cagatgtcca ggggtggtcg ccagcgccac gagcaccgcc cggcgggcct cggtcatccg
    28861 ctcgccccgc tcgcggagcc ggacaccgac gctggacacc agctccgcga tgtcgcggct
    28921 catcgcccac cggcgaggtc gggcgccgac tcgcccacgg aggcccggcc ctggccggtg
    28981 ctgcgcgagc gcaacgcggc cagcaacgcc aggatgatcg caccgctgac cacggcgaac
    29041 ccgagccgcg gctgcaacgg cacgacgatg cccgctgccc cgccgatgac ccagaacgtc
    29101 tgcagcagcg tctccgacca cgcgaagacc cgcccgcgca ggcggtcctg gacgtcgcgc
    29161 tggatgagtg cgtccaggtt gagcttgccg agctgaccga acagcccggc gatcaggccg
    29221 agcgcgacga ggctccatac ggcatacacg aaggtcgccg tgacggccgc ggcgacggcc
    29281 accccgagca ggatcgtggc cagcagttcc ggcggcggat tgtgcttgcg gttgccgatg
    29341 aaactgccga gcgcgttgcc cacgccggcc gcccccgcga cgagcccgac gacgagcgat
    29401 ccgctgatgc cggggatcgg gtgctcgcgc atgaggaaga cgaggaagag ggtcaggaag
    29461 ccggacagca tccgcgcccc cgtgagcagc cacaggacat aacgcacgct caccggcagc
    29521 gcccgcagcc ggcgggtcgg ggaggcggcc tcgtcaccgg cgaagtcgga ttcgccgcgg
    29581 tcggagtcga cccgggacgg cagccggatc gccagcacgg tggcgacgat gtagaggacg
    29641 aaggcggccc gcagcgacca gtcgggaccg atccgcgaca acgccccggc gatgccgccg
    29701 ccggccatca tcccgagcag gccggcgatc gtgagccggg agttggcctt gaccagcgtg
    29761 atgccctggg ggagcaggcg cgggaccgcg gcggcccgcg tcacggcata cgacttgctg
    29821 gccacgagcg caccgagtgc ggtcggataa agccacaccg agtcgtcgac gatcgccgag
    29881 gcgagcaccc acgcgaggaa ggcgcgcagg gccagcgtgg tgccgatcgc ccaccgccgg
    29941 ccgtggttga accggtcgag cagcggcccg atgagcgggg cgagcagcac gaatggcacc
    30001 atcgtgaggg ccaggaagag agcgacctgg ctgcgggcct cgtcggtcgg gagggcgaag
    30061 acggtgccgg cgagcgagac ggtgagtgcg gcgtcgccgg cgacattggc cgtatgcagc
    30121 tggatcagcc gggcgagccc ggtcgcgtcc gcgccgtgcg ccgtcgcgaa ggcctgatag
    30181 cgccgcgtcc cggcgcgggt gccggccccg acgacccggg ccgccccctg gctcccgcgg
    30241 cgcacggcct ggcccacccg ctgcgtgcga gtcgggggtc cgggaggcgc gaccggctcg
    30301 gggtccggct cataacccca gtcctcgtcg ccccactccg gctcctgcgg cgtcggcgcc
    30361 ggcgcagcac gccgccaccc cggcgggggc ggcggcggtg gcgacgggct cacagaccaa
    30421 tcctgacaaa tcgcgccgac aaccccaagg gtttccgcgc gtccttctgt cttacttggg
    30481 cgggtcggag aacatcagcc acaggtagcc gtatggcgac gcccgccggt gggtcttggg
    30541 ggcgtaatcg gccacgtgct acctccgggt caggtgtcaa accgtaacgg agggccgggc
    30601 cgtccacggc acttagcgcc gaccccggat ctgcccagcg cccgagtgcc cggatcgccg
    30661 ctcgcgggcg gtcgccgctc agacgtctcg gctcagacgt cgcggctcag acgcggtcgc
    30721 cggcgctgac ggtctgaagg atctccgggg cacggctcag atagcgctcc gccgagaacc
    30781 ggaccgagaa ccagagcagg acgagggcat agagcgggcc gagcacgagc gtcgcccacg
    30841 cgtacatcga gtcgaccgtc tccccgagca cgaccagcac gatgacgggg atgcacagcg
    30901 ccgcgccgcc cagcatcgtc atgagcgacg ccgcgaacgc gcggccaccc tggcccggga
    30961 cgcgggaggc gaacagcgag gtccggctct ggggcacggc atacggcacg taggcggagg
    31021 cgaacagcgc cgcggcggtc gaggcgatca ccgcgccaaa gcacagcccg agcatgcccg
    31081 gcaggtgcga ccagccgtcc cggacgacca cctggatgag cacccccacg aggctgatga
    31141 ccagccccgg cacgatggcg gcgatcgcgt gcccccacag ctcgccccgg gcgcgggccc
    31201 ggtcggcgaa ggcgaccagg tgcagccaca gccccgaacc ctcggtggca taggacgtcg
    31261 cggcctgcat gccggccatc gctgcggcga aggcgacggc ataccccgac cgttcgcttc
    31321 cggtgaccag gaagggccac ccgaccgcca gcatgacgat catcagcagc ggcaggcgcc
    31381 gcatcgggtc gcgccaggtc atcgtcgcat cgcggccgac catcagctgc gcggtcgagc
    31441 gggtgtcggt ggcgaccgcg tcggcgtcca tgagcccgcc ggccacggcc gaggactcgg
    31501 tcgtcgagcc gatcgtcgtc aggctcttgt gcagggcccg gccccaccac acccacagcg
    31561 cgacgaggaa ggcgacggcg atgagggcgc ggacggcggc agacagccag cggccctcgt
    31621 cgagatccga cgggatgacg gccagcgctc cgggaggcgt ccaccccagg acgcggccga
    31681 cggagccggc cagagagtcg gcgaacgacc ccgcccgggc gccgcccgcc gcgccggaca
    31741 gcacgacgta gacgacgaac agggcgaagg tgacggccat gccgaggtcc cggccgcgtc
    31801 gcgaacccat gagggaggcc atcgaggcgg cgagcgcgcg cgacccgatg acgcacatca
    31861 gcagcacccc cagcacgcag gcgagggcga ccggcagcag cgaccagctc cggctcaggc
    31921 cgatggcggc gccggtcagc gcgattgcgt tggcgagcgg gagatagccg acgagcgagg
    31981 agacgagcag gccgcgctgc agccggccga ggggtatcgg gagcagcgcg aagcggcgcg
    32041 ggtcgaccgt ctcgtcgacg ccgaacgcca ccagcggcat caccatccac gcgagcagca
    32101 ggctgaagaa gaagctggcc agccccagag gggcggcgtc cggtgcggcg cgcaaggtgg
    32161 cgaaacccag gccgaccagg ccgcccacga cgatcgcggc gaggacgccg aggatcagcc
    32221 agaggatccc gaggtcgccg cggttcatcg cccggttgcg gatcaggcgc agcttcagtc
    32281 ctgcgaagag cgcaaccatt ccaggccccc ctcggcgatg tcccggccgc cgacgatgtg
    32341 gaagaaggcg tcgtcgagcg acgcggcgtc gccccgcacc tgggcaaggg gcccggccgc
    32401 gacgatattg ccctcgttga gcacggcgac gtgggtgcag ttgtcctcga cgaggtccat
    32461 gacgtgggag gacaggacga ccgtgcggcc gccgcggaca tacgagtgca gcacctcgcg
    32521 gatgatgcgc gccgagacgg gatcgaccgc ctcgagtggt tcgtctagca gcagcagcgg
    32581 cggcgagtgc agcagcgccg cggcgagggt gatcttcttg cgcatccccg tcgaatagtc
    32641 ggcgacgagc ttgtcgccgg cgccggtcag gtcgagcacg tcgagcagct cgccgctgcg
    32701 ttgggtcacg acctcggtgg gtatgccgcg cagccgcccg agataggaca gcaactcggc
    32761 gccggagagc cgttcgaaaa ggcgcagccc gtcgggcagc accccgatcg cctttttcgc
    32821 ggccaccggg tcggcccaga cgtcgacgcc gtgcacccac acctggccct ggtcgggccg
    32881 cagcaggccg gtgaccatgg agagggtggt cgtcttgccg gcgccattgg ggccgacgag
    32941 cccgtagaag gatccggcgg gcacgtcgag gttgatgccg ttgacnnnnn nnnnnnnnnn
    33001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33061 nnnnnnnnnn nnnnnnnnnn nnnnntcggt agtatggacg tgcactcgcc cgtcgcggcc
    33121 gagcgctgtc ccttgccggg cgacataccg ctccggcagg cggccctcgc cgatgagcca
    33181 ctgcgccata ccggcgttca gggaaccggt caccggatcc tcgcctgcgt cgcggccgtc
    33241 cgagtagaag gcgcgcacct cgacctccac gccgtcaggg ccgctgtcgt cgtgccggcc
    33301 gaggacaccg accttgagcc cacgcatcct cgtcggatcg gggtcgaggg cgagaacgtg
    33361 tgcggcgctg tcgatctcga gtccgatcca gcccggcccg ttgtcgatcc acgcggcgtc
    33421 gaggatgtga tcgggagtga gcccgagcgc ctcgccgagg ctccgaagcc gttcgggctc
    33481 caccggtccg ctgcgcagga gcgggggagc ggcgaaggcg agccgctcgg catacgacac
    33541 ctcgatcagc ccgaccgcac attcctggag gatccggccc tccccctgcg gtatgccgcc
    33601 cgcctcccgc cacgcgtgcg ccgacccgat cgtggggtgg ccggcgaacg gcagttcacc
    33661 cgcgatcgtg aagatccgca cccggtagtc cgcgcgcggg tcggtcggct gctgcaggaa
    33721 tgtggtctcc gactgattgg tccagttggc gaaccgggcc atctgcgcat cgctccagcc
    33781 gtcggcgtcg tgaaccacgg caagagggtt gccccgcaac gggaccgcgg tgaagacgtc
    33841 gatctgacgg aacgcgggca cagggcccag tctgtcagcg gccgatcagg agttgctcgg
    33901 cgggcgcgtg gtgccgttcc aggtgttgcc ctgcttggcc cagtagccct tgaggtactt
    33961 gccgaggacg acgcggccga gccggttgcc actgaaggtg ctgccggcga tggcaccgat
    34021 gcccagaccg taggccgtgg aacccttcgg gttggtggcg acattgttcg tcacgatgga
    34081 gtagtgggtg ctggtgtcct tcgacggcag cgtcagctcg cggtccttca tccggaagcc
    34141 gaaggcgatg gtggtgtagc ggtcggcgcg gttgttgcgc cagacgttgc cccatccgga
    34201 catctcgtcg ttgtagccga cgggcacgtc gcgcacgagg ttgttctcga agatgttgaa
    34261 ggaggcgtcg acgtcggtgg cgaagccgcg gccgtcgtca ccgaggtcgt ggacgtagtt
    34321 gttgtcgacg cggttgtaat tggaggaggt gcccagccgg atgccctcct gcatgtagtg
    34381 gttggtgctg gcgtcggcga tgtgcgagac gtcattgccg gtgatccgcc agaaggtgct
    34441 gaaggtcagc acccagatgc ccgggccggt gccgcagtcg acgcgggtga tcgtgttgtc
    34501 ctggatcagg cccgagcgcg cgagggtggc gtggattccg cggccggtga cggtgttgcc
    34561 gatcagctgt atgccgtcgg cggggttcag cgtccggccg atcttgctct cgacgtaggc
    34621 gatggcgccc ttgctggccc ccgggtcgtt cgtggaggtg ccggggaccc gacgcctttc
    34681 ctgccagttc ctcttgtcca ccaggctgtc ggtccacgtg aaggcgtcga agcccccgcc
    34741 gccgttgatg tagacgccgt tgtggatctg cagaccggag atcgtccagt agtccgcccc
    34801 gcccttgacg aggatcgtgc ggtccgaggt cggcttcatc gaggagcact tggtggccgg
    34861 ttgcacggag gtgagtttga cgggaccgtc gccggcggcg gtcagggtga tcggcgcccg
    34921 ggcggtgccg gagtgcgtga tgctgatctg ttggttgtag gtgcccttgt ggaccaggat
    34981 cttcgcgccc ggcccggacc ggtccacggc cttctggatc gtgcggagcg gcttggcggc
    35041 cgtgccgggg ttggcatcgc tgccggtggt ggccacgtgg atgctcgcgc cgtcgaggcg
    35101 gccgccctcc gggatcgacg cggtcgcggt ggtgccggcg gcgaacaccg gaatggtcaa
    35161 cagcccggcg cccaggcggg cagcgatcgg caatgtgggc atgggtcccc ctgtaagcag
    35221 cacgttgttt tgggatttgg gaggcaaact accgcgcgga cccccgtcgg caaagccgtg
    35281 tccgcgtgag gtgacctgcg ccgggggtcg gtgacgctga gcgaagactt atcccggcca
    35341 cagcttcatg acaggttcgc ggcccaatct gaggccatca gggagagcgg agatgccgct
    35401 ctcgacctcg cacaaggagc atgaagacta tggcttccag caagctcggc aaaggtgcgc
    35461 tcgccgcgag cgcggccggt ctgctcattg ccggcggcgg cgcgatcgcg tatgccgcga
    35521 cgggcgactc gacttccacg accactccca cgtccaccgc cagctcgtcg acgtcgagcg
    35581 gtctgcccag cctggacaac ggccagggcg cgaacaacgg ccagaacaac ggccagacag
    35641 gccagcaggc accccagggc ggcatgggcc gcggcggtat gggtgacggc cagggcatgg
    35701 gcggccacgc gcatacggcc gtgaccggcg atgagttgac caaggtcaag gacgccatca
    35761 aggccaagga ttcggccgtc accatcacgc aggtgatgaa ggacgaggac gggtcgtatg
    35821 acgcgttcgg caccaaggac gggcagatgg tgcgggtcga ggtttctgcc gatctcaaga
    35881 ctgtcgaggt cggccggggc ggcatgggcc gcgggggcat gggtgacggt cagggcatgg
    35941 gcggccacgc gcatacggcc gtgaccggcg atgagttgac caaggtcaag gacgccatca
    36001 aggccaagga ttcggccgtc accatcacgc aggtgatgaa ggacgaggac gggtcgtatg
    36061 acgcgttcgg caccaaggac gggcagatgg tgcgggtcga ggtttctgcc gatctcaaga
    36121 ccgtcgaggt cggccagggc gggccgggta tgggccgcgg cggcatgggt ggccaccacg
    36181 gcggcgggat gcccggccag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36301 tggccccgct tccgtgaaac ccggcccggc ttgtggtcga cgtgattcac tgccccgtat
    36361 gccggagcct ctcgccgacc agttccgggg ccacttcggc caccgccagc acctgtatgg
    36421 cgtgttgctc gatcacctcg cggacgacct ggaggcgggc gggccgacag cggaggtctg
    36481 ccgcgaccaa ctcgatgcgg tgcgggccga tgcgattcaa ttgcgtttgc tggcagcggt
    36541 tttccgcatc gttctgcgtg gcgaagcacc acagctgacg cccttctatg cctgcctggg
    36601 tggcactgcg ccggcggagc agtgctggcc ggtgctgcgc gccgtgctgc ctgaccacgt
    36661 cgccgaactc cgggccgggt tgacccggcc gccgcagacc aacgaggtgg gacgctcggc
    36721 ggtcctggtg gccggcttgt tcgaggcggt ccggcgcacc ggtctcaacc gggtccggct
    36781 cctggagctg ggggcctcag ccggattgaa cctgcatccc gaccgctatc gggtgtcggg
    36841 tccagggtgg acctgggggg agcccgattc gcccctcgcg ctcgataccg aagcggcgga
    36901 catccgcccc ctcgatctga caatcgtccg tcggggcggg tgtgacctcg acccggtgga
    36961 tgtgaccacc caggagggcc ggatctacct gacctctttc gtctggccct ggcagctcga
    37021 acggcatgcc cgccttgcgg cggcgttggc cgtggcgcag gcccaccccg ttcgggtcga
    37081 ccgggctccg gcgtcggaat gggttgccgg gcaactgaat cgaccgccga cgccaggtgt
    37141 gctgacgatc gtatgggagt cgatcacgcg gcaatactgg ccggctcacg agtccgctga
    37201 cgtcgacgcg cacctggccg aggcccgccg tcgtatgccg ctcgcccacc tgtcgatgga
    37261 aggcgtgcct ccgcgacagg ggaccgacgg ttatgacatc cggcgagacg gccccgtgct
    37321 gcgtctggac ggcgagccgc tagcgcgtag ccaccaccac gggccacccg tcctgctgga
    37381 aaacgtgatc tccgaaacat tcaccggcgt gcgcagcata cccccgtagg tatcattgtg
    37441 gagtcatgtc aacagtggtg atcctgggtg ccggaaccgc tggcacgatg ctcgccaaca
    37501 aactccgcaa ggccttgccg cgcgactggt cgatcaccgt cgtcgacaag gacgacgagc
    37561 accactacca gcccggctac ctcttcgttc cgttcggcac ctatacgccc gagcgtgtcg
    37621 tgcgttctcg caaagcccaa ctcgaccgtg gggtcgactt cgtcatcgac gcggtggcgc
    37681 acgtcgaccc ggtgcatcag caggtgcggc tggccagtgg ccggcagctg tcctatcaat
    37741 ggctgatcat cgccagcggc accgtgccgc gggccgatca gacgccgggc ctgctggacg
    37801 aacgagtttg gcgcacaagc gccttcgact tctacacgct cgacggagcg gcggcgttgc
    37861 aggatgcgct ggagtccttc gagggcggcc ggctgctggt gcacatcacc gagatgccga
    37921 tcaagtgccc ggtggctccg ttggagttcg ccttcctcgc cgacgactac ttccggaagc
    37981 gccggatgcg gcacaaggtc gacatcacct acgtgacgcc cctcgacggc gcgttcacca
    38041 agcccgttgc gtcggccgcg ctcggagatc tcttggagga caagagcatt cgcgtcgcgc
    38101 ctgacttcgc gatctcgcac gtcgacccgg agaagcaggc catcgtcggc tacgacggtc
    38161 gcgaactccc cttcgacctg ctcgtgaccg tgccgctgaa catgggccag cagttcatca
    38221 tcgactccgg gctgggcgac gacctcggct tcgtcccagt cgacaagcac accttgcgct
    38281 cgcagatcag cgaccgcatc ttcgccatcg gtgatgccac caatgtgccg acctccaagg
    38341 ccggcgccgt cgcccacttc gaagtcgagc atctcgtcaa gaacctcgaa gccgcgatcg
    38401 ccggcgcgcc gctgccgagc aagttcgacg gacacgccaa ctgcttcgtc gagtccggtc
    38461 acggcaaggc gttgctcctc gacttcaact acgacaccga gccgttgagg ggcaccttcc
    38521 cggcgtccaa ggtcgggccc atgaagctcc ttggcgagtc ccggctcaac cacatcggca
    38581 agctggcctt cgagaagatc tactggaaca tgcttttgcc cggcaagccg ctccccgttc
    38641 cgacggagat gtcgatggcc ggaaagatcc ctgcaggaca taggagttga cagcggtgcc
    38701 caccaacacc atcgacggcc acacgttcgc cgtcaacgac gaggggttct tcacgaaccg
    38761 cgaggagtgg agcgaggacc tcgccgtcat cctcgccgac ctcgtgggca tcgagcagat
    38821 gaccgaagcc cattgggcgc cgttgcggtt catgcgggag gactccgcga agaccggcgt
    38881 caccccgacg ctgcgccgca tgcaggccgt cggcggcttc gacgtcaagg acctcttcgc
    38941 tctctatccc ggcaagccgg ccaagaagat ggcctacctg gccggcctcc ccaagcccgt
    39001 cggctgcgtt taaccccacg aactgagtga cgaaatgcga actcttccag agggattcat
    39061 catccccgac ttcggcggca gcgccgccaa ggcgaccacg acgagccccg agccggcagc
    39121 agggggtgag gcgacggcat accgcggccc ccgcaagctg gctctcatct gctccaaggg
    39181 caacctcgac atggcctacc cgggactcat cctcggcaac gcggcggcgg gcgagggcgt
    39241 cgaggtgcac atgttcttca ccttctgggg gctggacatc gtcaacaaga agacccacaa
    39301 gaacctcaag ttcacgatgc tcggcaacac cgccatgcat atgcccgaac tgggttatct
    39361 gcgaacgggt ttggagcacg cgtcgatgcc ccagctcatg gggcagctgc ccggcatgac
    39421 ggccatggcg accaagatga tgaagaagca gatggccgac ctcgagatcc cggacgtgcc
    39481 cgaattcctc gacctgctct ccgccgcggg cgtgcacatg tatgcgtgca agctctcctt
    39541 cgacatgatg aagatgatcg aggccgacct gcatccgggc gtcgagggcg tcatcagcgc
    39601 cgccgacttc atcgagatca gcgagggcgc gcagaccatc ttcatctgac ctccggggaa
    39661 ggctgtcgga ccggtcggcc agactgctga gcatggacat tcgtgacctg cgcccgcccg
    39721 tcgaggacgg cccgtggccg cccctcaccc agctgccgcc gatgaccttc gagccgttgg
    39781 cccgggccac ctcgtggcag cagctgatct atgcgcaggg cggtggcttc cgtccgctgc
    39841 tgatggacgt gcacgtgccg gcgacgcccc ggccggcgcc ggtggtcttg tgggtgcacg
    39901 gcggcggctg gggtgagggc gatcggcgcc tggtgcctct gcagtggggg cagcaggagt
    39961 tcttccaaca gttcgtcgac gccggcatcg cggtggcgac gtgcgactac cggctgatcg
    40021 cggaggcgcc ggtcgaggcg atggtccagg acatcgtcgc tgccatccgc tatctccgcc
    40081 ggtatgccgg tgagctggcc ctggacgccg atcgcgttgc cgtgtgggga gattcggccg
    40141 gctcgcacct gagttctctc gcggcgctcg cgggcagcgc accggatccg gatccggcga
    40201 tcctcggctc gcagggcgta ggcgagggcc gcgccgatgt gaccggcatc gtctggtggt
    40261 atggcgcgtc cgatctgacc gtgctgcccg agctgtgcga attcctctgg gccacagcga
    40321 cacctgagga acggacaacc ctggccgagc gctattcgcc ggtcacctat ctgcgcgccg
    40381 actcgccgcc cctgttgatc atgcacggcg accaggacac gatggcgccg ttcgaccagg
    40441 ccgtgcgcct gcacgaggcg gccgtcgccg tgggtgcccc gagccggctc ctcgtcgtgc
    40501 ccggcgccga gcacgccttc ctcggcgccg acatccgcgc ccagtggcag accggcctcg
    40561 acttcctcgc cggcctcttc gccggtggcc gcgcgcccgc tgccgcccga gcttgctcaa
    40621 gtcttggaaa acgttctcct gacgcttgcc cgcggagggt cgttgaccat cgaaacgatg
    40681 ccggatctgc tcacgaccac ccaggccgcc aagctcctgg gcgagtcgcg cccaacactg
    40741 atcaagcgta tcgagtctgg agaattgccg agcacaatgg tcggcagcca ccataggttg
    40801 cgcaccaatg acgtgctcgc cctgcgcagg atgcggctgg aacagcgccg ggccgccgcc
    40861 cttgagttgc tcgacctgga ggacaagttg gggctctgac actcgcccgg atgcgagcag
    40921 tcacgctcaa gcctcaactg tcactttcat aacacgacct agggcccgtg aacgtgcaga
    40981 cctaccggtg ggtcactgcc ggggcacagc gagcgcacag cgggccggcg caccctggtc
    41041 gtatgaccag tcgacacggc ttctcgggaa ggcggcggca cggcgccgcc gcagtggtgg
    41101 cgctcctggt cctcggcggc tgcggcaccg cgactggccg cacgagttcg ccgccggtca
    41161 cgtccgcggc gacgtcggca accgtcgtct ccgtcggcgg ggtcaccgat gtctcgacct
    41221 atgcgacctg ggccgacccg cggatcaccg agtccagcag cctcacgcgt gtcggcgacc
    41281 acctgatcac cgtcaacgac agcggcgacc ccgcggtcct ctatgtctcg acgggcacga
    41341 gcggtcagat caccggcacg atcacctacg ccgacgccgc gccggacgac gtcgaggcgc
    41401 tcgcgacctc accggacggg acgatctggg tcggcgacat cggcgacaac gcccgcgccc
    41461 gctccacgat cgtcatctac cgcctgccgc ccatcaccca gtgggacggc gacacgacgg
    41521 tcaccgccca acgctacgaa ttcgcctatc ccgacgacgc ccacgacgcc gaaacccttc
    41581 tcgtgcaacc tgattccggt gatctcttcg tggtcaccaa ggacgcctcc ggcggtgtcg
    41641 tgtatgccgt gccgaccctc gacgcgaccc gaaccgtgac cttggagaag gtcgcgcagg
    41701 tcgcggcgac ggtgaccgac ggcgtcttcg acgcgagcgg cgagcacgtg ctgttgcgga
    41761 cctacggtga ggtcacggca tacggctatc ccggcttcca gcaggtcgcg acggcgacgc
    41821 tcccggccga gcccaagggc gagggactca ccctcggacc cgacgcgggc acctatctga
    41881 ccgccaccga aggcgccggg actccggttc tgcggtggcg gtggtcggtg tgacgctcag
    41941 gcagtgactg gctgctgcag ctcggtcacc cagtcgtccg ggcgatccat cggcgtcgcg
    42001 atatagacct cgcggcagcg gccggtgttg gacagaccct gcgcgccgac atactgctgc
    42061 agcgcctgcc acgtgtcccc gatggtctcc acgtcgcccc ggtggacgac ggtgatggcg
    42121 cgcgggacgg ccgcgagttc gacgtgctcg gtgccgggca ggagcggcag accctcggtc
    42181 tgcgggatgc aggcggcgat ctccatgggg tcgccgnnnn nnnnnnnnnn nnnnnnnnnn
    42241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42301 nnnnnnnnnn nnnnnnggag accgaggcgt cgaggatcgg gccgatctgt tccagggtga
    42361 agcccaactc cttgagagcg atcagccggt tgagccgcga aagctgttcc gcggcatacc
    42421 agcgatagcc cgaggacggg tccacgtgga caggctcgag caggccgagg gcgtcatagt
    42481 ggcgcagcat gcgcaccgac acctgaccgt gtagggcgaa gtctccgata ctcaacatgg
    42541 cgctgtcagc gaatcgcctg acaccgtgtc agggtcaagc gctccggaca cgtttctcga
    42601 aagtcttctc agagatcgaa gttcgtcggc atgaggatga cgtcgcggat ggtgacattc
    42661 cgcgggcggg tcaacatgaa gaggatgacc tcggccacat cgctgggctc catcagcgag
    42721 ccggactcct tcgcctcgcg caacttgtcc tcgggccagt ccgagagcag cgcgctggcc
    42781 acggggccgg gggagaccga gccgacgcgg atgccgtgct tgaagtactg acgccgtgtc
    42841 gtctgcacga agcagttgat cgcccacttg ctcgaggcgt agaccggctc ccacggcgtg
    42901 gggtaatgcg ctgcaagaga actcgtgacg aggatgtcgc cgccgcccgc ctcgatcatg
    42961 tggggcagga catcgtggac gttcttcatg acgacattga cgttgaggtg caacatccgg
    43021 tcgatggcct cgttgctggc ctcggtcaga tcgccgccga catagatccc ggcgttggag
    43081 tggaagatgt cgagcttgcc gccgaaggat tcgagcaccc gggggatcag tccggcgcac
    43141 tgcgccgtgt cgagcaggtc ggcctggatg ggcacgacgg cctcgccgtg ctcgtccttc
    43201 atcttgttga gcaggttctc gtcacgatcg atgatggcca cccgcgctcc ggccccgatc
    43261 atggcctcgg cgctggccct cccgatcccg gcgcccgccc ccgtgaccgc tgccgtcttg
    43321 ccgctcaatg cacctgctga catcgcctgc tccttcgggt ggtcgagatc atccgtgctt
    43381 catcgaagca cggatcacgc ccgcctgccc acccttacgg gacgacggcc cgcaccgcct
    43441 cggcgaggcc tccgggctcc tgctcacgca aggaataccg agcgcgcacg aggggctttt
    43501 cgaccgtcag cacgcggctg agatcgatcg gggtgcccgc caccacggca tccacgggca
    43561 tcgcttcgat cgtggcctgg aggtcgtcga cctgaccttc cccgtagccc atggccggca
    43621 ggatcccggc ggcgttcggg tacttggcgt aggtcgcggc gatggcgccc acggcatacg
    43681 gctgcgggtc gacgatctcg gcgacccccg cggccttcgc gccgacgaca cccgcgccga
    43741 agctcatcga cccgtgcgtg agggtcggac cgtcctcgac gaccacgacg cgtttgccgg
    43801 cgatcgcctc cgcgtcgtcg actgtcacgg gtgagtcgca gcgcagcacc gtcgccgtcg
    43861 gattcatctg ccgcacactg gcttccacgg cctcgatgtc ctcgggcgcg gcgctgtcgc
    43921 atttggcgac gatgacgaca tccgccattc gcacgttcgc ctcgccgggg tgataggtgg
    43981 cttcatgccc cgggcgcagc gggtccgtga gcacgatgtg gaggtcgctc ttgtagaacg
    44041 ggatgtcgtt gttgccgcca tcccagagga tgacatcggc ctcgtcctcg gcggcacgca
    44101 ggatctgctc gtagtccacc ccggcataga cgacgaaacc gttgtcgatg tgtggctcgt
    44161 actcctcacg ttcctcgatc gtgcactcgt gcaggtccag gtcctcatag gtcgcgaacc
    44221 gctggcaggc ctgcttggcc aggtcgccat agggcatcgg gtggcggatg gcgacgacgc
    44281 gctggtccag atcggcgagg atcttcgaca ggtaccgggt ggtctgggat ttgccgaccc
    44341 cggtgcgcac ggcggtgatg gcgatcacgg gtttcgtgga ctccaccatc gacgaatcgg
    44401 cgccgtgcag aaggaaattc gcgcctgccg cgatcgcccg cgaggcaagg tgcatcacct
    44461 gttcgtgagg caggtcgctg taggccagcg cgacggtgtc gatgcgttcg ttctcgatga
    44521 gttcttccat ccgcgcctct tcgtagatgg gtatgccgcc cggatacagc gaacccgcca
    44581 gttcaggcgg ataggtgcgg ccttcgatgt ccgggatctg ggtcgcggtg aacgcgacga
    44641 cctccttggc cgggtcgtcc cggtagatga cgttgaagtc gtggaagtcg cggccggcgg
    44701 cgccgaggat gaggatgcgt tcgcgggcct ggggcagtgc ggacactggg gcggtcatgg
    44761 tgatctccgt cgcggctgcg gtcgaccgcc ggaaccgact tgccctgact ttgggcagtc
    44821 ctggctgcgg gacccgcctg tccccagcct actcggcgct tttcggccag cccggacggg
    44881 gtcagcgggg ccgagaggtg cgtcagtcaa gccccgagtc ctatcgggga gcttcgtatg
    44941 gttggctaat ctgccgttat ggcacatacg ctgctcgtcc tgcgacatgc caagtcggac
    45001 tgggccgtgc cggtcagcga tcgcgaacgg ccactcgcga agcgtggtgt gcgccaagcg
    45061 ccgcataccg gccggtggat cgccgacaac gatcttgtgc cggatgtcgt catcgtctcc
    45121 ccggccacgc gcgcaatccg gacctgggaa ctcgtctgcg gcgcctggac cgcccggccg
    45181 acggaggtca ggatcgacga ggcggcctac accttcggcg cgggcggttt gctcggcctc
    45241 gtgcgcgcga cgccggaatc cgtcgcccgg ctggcattgg tcggccacaa cccggccgtc
    45301 gaagacctgg tcgccgttct caccgacgag tccgtgcgca tgccgacctc cgccctggcc
    45361 gtggtcgacc tcgatcgctg gcagggcgcc ggtatgcggg acggttgcct caggtatgcc
    45421 ggtcgccccg ccgacgaatc gggtttctga tccgaactgc caagtgccgt gactagacgg
    45481 ccgcttcgcg ccagcgcgga ggcaggtagg cgcacgtcgg gtcggaggcg agcggatcgc
    45541 cggtgacggc atacgcgtgg gaccgggagc cgccgcacac ctggttgaac tcgcacaccc
    45601 cgcacttgcc ggcgaagtga tcggggtcgc gcagcgcctt gaggatcggc gcctcacgat
    45661 agatctcgcg gaaggtcgtg ccctcgtcgc ggacgttgcc ggcacgctgc ggcaggaagc
    45721 cggaggggta gacccacccc tgatggtcga cgaagacgaa gccgcgtccg gagttggtgt
    45781 cgatcggcgg tcgtgccggc cgggtgggga gttcgcgtcc ggcaaggacc tcggatgtgc
    45841 gctgggtgag agcggcatac agctgtccgg tggcgaacgc ctcggcgccg tgaccctcct
    45901 tctcggctgc ggcgcgctgc acgacgacgc gccgatagtg ctgcgcctca gtggttttca
    45961 ccggcatggt gtgcgagatg tcgaccatcc agtgcaggac gtcctccacc tggtccggac
    46021 tcaacgcctc cagcgccttg ccccgcccgg tcgggacgag gaagaagaga ctccacaggt
    46081 ggaccttgag atcgagcagc agcgccaaca gcttgggaag ttctgcgacg gttcggctgg
    46141 tcacggtcga gttgacctgc agccggaagc ccgcgtcgtt gatgaggccg gccgagtcga
    46201 tcgtgcgctg ccacgtgccg ccgaaaccac ggaaggcgtc gtgcacggcc ggggtcgccc
    46261 cgtccaacga caaggacatc gccgacgccc ccgcctcccg catcgcagcg agccgctctg
    46321 cggtgacatt cggtgtggca gagggggaca gggccatgtg caggccgagg gaatggccgt
    46381 atgccgtcag ctcggccagg tcttcacgct cgaaggggtc accgcccgtg aggatgacga
    46441 tcgggtaggg cttgccgaag cccgcgatgt cgtcgaacag cgctttgccc tcggttgtgc
    46501 tcagctccaa cgggtttcgc tgatggatcg cctcagcacg acagtgcgca cacgcgagct
    46561 ggcaggcacg cgtgacttcc cagatgacga tgaacggccg ctcgctcaca tcgtggcgga
    46621 tctgccgcac gacccggcga tcggcctgga gactgctcac ccgtccatac catcatcgat
    46681 ctgcgttgcg cgcgtagtga ggggtgcctc agctgacgta gaggcagaac gggtggccgg
    46741 cggggtcgag cagcacgcgc acgtcgtctt gcggctggta tgccgcgagt tcggccccca
    46801 gggcgatcgc acgttcgact tcggcgtcga gatcctcgac ctcgaagtcg aggtgcgact
    46861 gcatctgttg gccacccggg ggtgtcggcc atacgggccg cacataatcc gggttgcgct
    46921 gcacggcgag ataggcgacc ccttcgccca ggtcgagagc gccgtcctcc tcatcggcct
    46981 tgtagatcgg ccagcctcgc agcttcgagt agaagttcag caggggtgca acttccgggg
    47041 cgtcgagcac cacgccccag aagtccttcc gtgcccaacg gtccgtctcg ggagtgtccg
    47101 tcaccagtcg gctccttcgc agtccgtgcc gggggtggtc tcgacctgga ttgtggcatg
    47161 cgcgatgccg aaccggtcgc gcaacagggc ggtcgcggcg gcgagcgggt ccccggctgc
    47221 gtcgccggcc cggaccagat gtgccgtggc gacattcatg cccgaggtca actgccagac
    47281 atgcacgtcg tggacctccg cgaccccgtc gaggccttcg agtgcggcga ggatctctcg
    47341 cggctccatc ccggcggggg cgtgttggcc gagcacggcg agcacttcac gaccgaggag
    47401 cagggcgcgc acggcgacga agagcgcgat ggcgagggcg atcacggtgt cccaccgcga
    47461 gtcgcccgtc gcctcgatga gcagggcagc ggcgatcacg ccgacggacc cgagcgtgtc
    47521 accgaggacc tcgaggtagg cacccttgac gttcaggctc tcgccggcga cggagtgcaa
    47581 caacgccaag cacacgaggt tgacgatcag gccgagcgcg cccacgagca gcatcgcctg
    47641 caaggccggc gccgtggcgt gatcggcgcg ctgcacggcc tcgatcacga tgtagacggc
    47701 cacgcccagc atgacgagca ccgcgatgcc cgacgcgaag acctcggccc gatacgaacc
    47761 gaacgtgcgc cgcccggtcg tgtccggccg tgtggcgatg cgggtcgcca cgagtgccgc
    47821 cccgagggtg acgacgtcgg cggccatatg gccggcgtcg ctgatcaggg cgagcgagcc
    47881 cgagaccaga ccagcgatca gttccaccgc gaagaagccg gagatcaggg cgaaggagat
    47941 cgcgagccgc caccggtgat gggccgccgc cgagcctcca tgggcgtgcc cgccgctcac
    48001 gtggacctct ccatggtcgc catcatcacc tgtggggcgt gtggatgggg gaatgcgcga
    48061 ggtctagcgt gagggttgga ccccacgagg aggctcgcag gggcggtgcc gccgcaacgt
    48121 cggaccgctt ggggtcgact ggacgaaagg gttcccgcgt catgaccacg ttgcacacca
    48181 tgggccagca gcattcccgc cgcagttggg ccgagcccta caagatcaag gtcgtcgagc
    48241 cgctccggac gaccacgcgc gaggagcgcg accgggcgat cgccgaggcg ggctggaaca
    48301 ccttcttgct gcactcgcgc gacgtctaca tcgacctgct gaccgactcc ggcacctccg
    48361 cgatgtcgga ccggcagtgg gccgggatga tgctcggcga cgaggcctac gccgggagcg
    48421 tcaacttcta ccgcctcgaa gagaccgtgc agaagtacta cggctacaaa cacctggttc
    48481 cgacgcacca gggtcgcggc gccgaaaacc tgttgtcgca ggcggcaatc acgccgggcg
    48541 atcacgtgcc gggcaatatg tacttcacga cgacgcgctt ccaccaagag aaggccggcg
    48601 cgaccttcca cgacgtgatc atcgacgagg cgcacgacac cgcctcgctc catcccttca
    48661 aaggcaatat cgacctcgac aagctccagg ccctcatcga cgaggtcggc gccgacaaga
    48721 tcccatacgt gtgcatggcg gccaccgtca atatggccgg gggacagccg atctcgatgg
    48781 aaaacgcgcg cgccgtgcgg gagttgaccg accgccacgg catcaaggtc tatctcgacg
    48841 cgacccgcgc ggtggagaac gcctacttca tcatcgagcg cgaagcgggg tatgccgaca
    48901 agtccctcgc cgaggtcctg gcggagttct gctcctacag cgacggcgcg acgatgtcgg
    48961 gcaagaagga ccacctcgtc aacatcggcg ggtggttcgc caccaacgac gacgagctct
    49021 tcgaggaggt ccgcaacctc gtcgtcgtct acgagggtct gcacacctac ggcggcatgg
    49081 ccggccggga catggaggcg atggccatcg gcatcgagga atcactcgac atgccctaca
    49141 tgcgctcacg catcggacag atccaatacc tcggtgagct gctcgaacag tgggacatcc
    49201 ccttcgtcaa acccgttggg ggacatgcga tcttcctcga cgcccgggcc ttctatcccc
    49261 acctggatca ggagcagttc ccagcgcaga cgctcgccgc caacctctac gccgactcgg
    49321 gcatccgctc gatggagcgc ggcatcgtct cggccggccg tgacccggaa acgggggagc
    49381 accggcaccc gaagctggaa ctgacccggc tgacgatccc gcggcgggtc tacacccagg
    49441 cccacctcga cctggtcgcc gagagtgtca aggcgtgctt cgacgcgcgc gattcggcga
    49501 cgggtctacg gctggtttac gagcccaagt atctgcgctt cttccaggcc cgcttcgaac
    49561 cgctgccctg acctcttccg cccgacacgg gcggggtcag tggtgttcgc cccgggcgac
    49621 gaggtccgcg tagtcagggt gacgttggat ccaactcttg atgaactcgc attgcggctt
    49681 gactcggcgg gtgccctcgc gcacttcgtc caaggcgaac cgtgccagcg cgctgccgac
    49741 gccctgcccc tcgaacttgt cgtcgacttc ggtgtgggtg aagacgatca ggtcggcaga
    49801 gaggatgtat tcggcgaagc cggcgagttc gccgtccagg tgcgcctcgt agcggtcctg
    49861 agcgggattg ttcgtgacga cgacatcgga catgcggacc tcctcggtga ggcctccatc
    49921 ctgacaggcg cacgtggggg aggcgcctgc tagagcgcgc ccgagtgctg cgctcgcggt
    49981 ggcgagggcc gggggcgcgt tgccgtcgac gtcagcaccg gccgaggatg gcggcgtcca
    50041 ggtcgcgatt cttcaggatg gtcgagaagc gcgccggccg gtccgcacag accttgctga
    50101 tcggtccctt gtactcgatg tgcaggaccg ccttgcccgc gttcagcatc ggacggtatc
    50161 gacagccctc gctgatgggc gagtgacagc agcgcccgca ggtatgccgt ggagtccgcc
    50221 ttcgtcagcg agaaaccggt tccgttctcg tagccgtcga cattgtcggg gtcgattgcc
    50281 tcgaatccct ttgccttgca cacatccatc cgcgtgccca tgatcggcag gagcactttg
    50341 agggcccgcg tatcgagcca gcgctcaccc ttccaccccg acaacggacg gcctgtgacg
    50401 cggtcgggga acgcgccggc gtcggagcgc cagtcctccc acgatcccgc gctgaggtag
    50461 cagatcgcgt gccggcccga ggcccgcagt gatgccgctg cggcccccgc gcgggtatgc
    50521 cggcgtggcc ggatccggtg gactcgacgg actcctcgcc gatccgggga tccaggcgtt
    50581 gggcgccgac gccacgcggc tgaccctggg cctcgcggtc tgggccatga tcatgggagc
    50641 cgtcacggcg gaagtgttct cccagtacgg cacccaggcg atcagcgacc cggcggccca
    50701 cttcgccgaa gtcgtggaac tggcggtcgg cgtcatcgcc ggcgggcaga ctggtcggtg
    50761 accacaccgg catacgacga aaggcaccgc catggctgac tcgccggagg actatctcgc
    50821 cgccgcaccc gaggccggcc ggccctggct gacggagttc tgggaccacg tccgccgccg
    50881 cagcccggag ttggagctgg cgatgttccg cggcgtgccg atgttcaagt tcgaacggag
    50941 ctatcaggac ggctatgtca tgttcaccgc ggccaagggc cacttctcgg cgcattccct
    51001 cgcgttcgag ctgatcgagg agaccaaggc cgagatcccc ggctccttcg gcggaaaggg
    51061 ttgtgtgagt gtgaaatacg ccaacgagga ggcgaagccg gcgctgaagg cgtatgtgga
    51121 cgaggtgctg cgggcggcgt cagcggagga ctgatgccga ccgcctatgt cgtcggcagc
    51181 ggtcccaacg ggctcgcggg tgccgtgcgg ctggccgagg ccggtctcga cgtcaccgtg
    51241 ctcgaacgcg ctgaccggat cggcggcggg atgcgcacct cggagaacac cctgccgggt
    51301 ttgctccacg acgactgctc cgcgttccac ccgacgggcg ccgcctcgcc ctatctgcag
    51361 tcactcggcc tcgaacgcca tgggctgcaa tggctgtggc cggagattca atacgcccac
    51421 ccgctcgacg acggctcggc cgggctggcc ttccggtccg tcgagtcgac ggccgagggt
    51481 ctgggtcgtg acggggcgtc gtgggcgcgg ctgttcgggc cgctggcccg gcagtatgac
    51541 gcgctggcgg ccgagctctt ccggcccgtc ctgcatgtcc cgcgccatcc gctcgccctg
    51601 gcccggttcg gtcccgccgc gctcctcccg gcgaccgtgc tcgcgcgccg gtggcaggac
    51661 ccgcaggcca aggcgctgta tggcggtgtc gccgcccatc tcctcggtcg cctcgaccgg
    51721 ccggtctcgg ccgcggtcgg gctgatgctc ggtgccgccg cgcacgcgta tggctggccg
    51781 gtcgccaaag gtggctcgca ggcgatcgcg accgccctcg ccgcccggct gaccgacctc
    51841 ggcggccgca tcgagacgag ggcggaggtc acggcatacg accagatcgg cgacgccgac
    51901 gtcgtcctct tcgacctctc gccaccggcg gtggcgcgga tcctgggcga gcggctgccg
    51961 cagcgggcgg cggcggcata ccggcggtat cgcttcggcc ccggcgcctt caaggtcgac
    52021 ctggccatcg agggcgagat cccgtggcgc aaccccgacg tcggccgcgc cggcaccgtc
    52081 catctcggcg ggacactcga ggagactgcg gcagcggagg cggacgtcgt cgcggggcgg
    52141 atgccgtggc ggccgttcgt cctgctgggg cagcagtatg tcgccgaccc cactcgctcg
    52201 gccggcgggc tcaatccggt gtatgcctac gcgcatgtgc cggccggctg ggtgggcgac
    52261 gcgaccgacg cggtcgtcgg ccagatcgag cggttcgcgc ccggcttccg ccatcgcatc
    52321 cgggccacct tcacgcgggg tcccgcggag ctggaggtgt acaaccccaa ctacgtcggt
    52381 ggtgacatcg cgaccggccg caactcgccc gtccaggtcg tcatgcgccc gcgcatctcg
    52441 ctgcacccct accgcaccgg ggccgccggt gcctacctgt gctcggcggc gaccccgccg
    52501 ggcgcgggtg tgcacggcat gggcggcttc cacgccgcag agacggcgct ggccgatctc
    52561 gagccccgtc gggaacgcgg aaacgcctga gcgccggcgc cggtcagggg cgctccaagg
    52621 tgtcgcgcag gagggcctcg agttcggcgt aatgccgttg cgccgcctgc gcgtcgtagg
    52681 caccggtgtc cgccatcgtg aagccgtggc gggcgccggg atacaccgcg gtcgtatgcc
    52741 gcacccccgc ctcagcgagc gccgcgtcca gccgggactg tgcctcggcg tccatgccgg
    52801 cgtcctcgtc ggcgtgaccg tagacgaagg cggcgcgggc atgcgcgagg ccccggtgcg
    52861 ggctgtccgg gtcgtccgtg gccaagcggc caccgtggaa cccgccgcag gccgcgatgc
    52921 tcgcatcgag gccggcggcg cgcgtggcga gccgggcgcc catgcaatag ccggtgacgc
    52981 cgatggcacc cggtgcgcac tgcggcaacg cacgcagcgc tgcgacgtat gccgggacgt
    53041 cgaccgccgc cgccgcgccg tcgagccgcc cgatcatcgg catgactcgt gcgaaaacct
    53101 ttgcgacagt gtccgggtcg cgcagatcgg cgggtgggga gacctccgcg acccggccag
    53161 aacgatagag agtgttgggt gccaggacga catatcccca cgacgcgatg cgatcggcca
    53221 tcgcctcgat ctgcgggcgt agcccgaagg cgtccatgaa gagcagcacc ccgggatggg
    53281 cgtctgtctc gtccggggtg gccacgatcg cctcgacccg ctccgtcgct accgggatct
    53341 cgatcagctg acgcatgcct cgaacctaac cgcccgatcc gggtctcgtc accgcggcaa
    53401 ggtcacggcc acggcgacgg cgacgcagcg cgcggacggt gtctcgtcgt cgccctgcgc
    53461 cccaggcaac ggcgccgtgt gttggcgcgt catgtcgcaa tgtgcgcaac agggcgctcc
    53521 ggcgacgcga tgctggcgga catgccggcc gacctgatcc gctccggtct tctgtccgat
    53581 gtcgcgcagt acgcgtatgc cgccgtcgtc cccgagggca ctcgcctcgt cttcctcgcc
    53641 gggtcatgcc cgctggacga gtccggtgtc acggtcggga cccgggacta cctgcgccag
    53701 gctgagcagt gtgtcgccaa cttgcgcacc gtcctgggag agctgttggc cgacatcacc
    53761 gacttggtca gtaccagggt gctggtcgcg agcacggacc agcaggatct cggcgccgcc
    53821 tggcacggat accgggctgc catcgcaccg cacgacccgc cgagcacact cctgggcgtc
    53881 accgtcctcg gctgcgacca ccagctcgtc gagatcgaag ccgtcgccgc gatccgctgc
    53941 tgaggagcga tgtcaccggt ctggcatcgc cggctgcagc gtcggctcgg tcacggggtc
    54001 gggtctgcaa catcgtcggg tgcttggcgc atcctcaccc cggtgcaggg ggtcgatgca
    54061 tcgagggccc gaggatatgc gtaacgacga cgcgagcagt cagcagggcg gcggtggtct
    54121 cacgaggag
//
LOCUS       DAOPJG010000003        47448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_791_length_47448_cov_7.318591, whole genome shotgun sequence.
ACCESSION   DAOPJG010000003 DAOPJG010000000
VERSION     DAOPJG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 47448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47448
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_47448_cov_7.318591"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1315)
                     /locus_tag="PKU75_00735"
     CDS             complement(68..1315)
                     /locus_tag="PKU75_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79429.1"
                     /translation="MTKHTYDDADLERMIRDGLRHHAGDAPSDLGAPLPGSGGSRSSG
                     SATAAAVPPSSPRWRWVLPAAAAVAALAVPGAILVANTLRDNDSGVTDPVAGASRSAS
                     TGADSSTTSESPKTAATADGRPAGIPADWRPESYGGVQLWVPPTWGWGGAPMAPDWGN
                     GEVLDCSEGRAFTVPGSSAYEFVPDGVPYVGRPVMMTDACVGSPDVHPKVDAVWFDAA
                     GVKAGTESYPDGVVRETRSVGEVTVTVFSKDAALRERILGSASSVTVDANGCPASPPD
                     VIRDSTPAGPTAPASLGICVYAAKGLLWSGAVGAKAAAAYGKAAGPHAPGATAGGPAN
                     RRGEDEVYLRLADAAGRMRWDRYDAAIGVLSPTDSGTVLVPATVANTAPWAKDNGGVK
                     AYVIGGGTALDAALQQYFRGILG"
     gene            complement(1312..1827)
                     /locus_tag="PKU75_00740"
     CDS             complement(1312..1827)
                     /locus_tag="PKU75_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HPF79430.1"
                     /translation="MVTTVDKAGVDRAGFETWVTPRLPGLLRFAYLVTGSRDAAQDAV
                     QGALAAAYETWDHVSTRAQPDQYVRRMIANSHVSQWRRIGRREFSVAEPRGGSAADST
                     EQIAEHDAVWRMCLGLPPQQRAAVVLRFYEDLDYREIARVLDISEATARSHIHRALIT
                     LRIQLTSEDLP"
     gene            complement(1884..2630)
                     /locus_tag="PKU75_00745"
     CDS             complement(1884..2630)
                     /locus_tag="PKU75_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HPF79431.1"
                     /translation="MLILLPPSETKARPSRGAPMRVDALSFPELTGRREEVIDAVINA
                     SGLEDAQRILYVPSTLGAEVEGNLAIRTRPARPALEIYQGVLYDALDAPSLSPGGRRR
                     AARRLVISSAVYGAVRAGDRIAPYRASMCAHLPEVGKLAHRWKESLDPVLSAAAGKGL
                     IVDCRSGEYAATWRPTGALAERWVAVRVPGASHMAKHTRGLVARVLCESDADPRRPAG
                     LIPLLENDFRIELHEPAKPGAGWTLDVLPH"
     gene            2672..4198
                     /locus_tag="PKU75_00750"
     CDS             2672..4198
                     /locus_tag="PKU75_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPF79432.1"
                     /translation="MAQPMRARYSDTGIAAVLADDRLLARFDAIRAEFTVPEAFPPEV
                     LAEADQIAGAPMEWPERDETAVPFLTIDPPGSMDLDQAVHIERAEGGFRVRYAIAYLP
                     AFVAPGGAIDGEAHRRGQTIYAPDERTPLHPPQVSENAASLLPDQVRAAYVWDMHVKD
                     DGEGDQKTVYRALVRSVDRFDYEQVQAMIDAGTDDDRVALLKEVGERRIAFEQRRGGA
                     SLPMPEQQVTQDEAGKFVLGFRPPLPAEEWNAQISLMTGMAAAELMVEARTGILRTLP
                     APDEQAVERFRRQAKTLGVQWQEGESYGAFLRRLDRTNPHHLALIYDATGLFRGSGYV
                     AFDGEVPPAPEHGALASTYAHVTAPLRRLVDRFGLAIAEALAAGRPVPEWVRSGIGVL
                     PEIMAATGRTASGVERACTDAVEAAVLSQYVGDTFEAVVVDQGQKGAWLLQLTDPAVV
                     AHAGTEVKVAVGDLVAARLTEADVTAGVVRFHITARKGAVPAPAGVPASPEDPLAPPA
                     "
     gene            4214..4597
                     /gene="rnpB"
                     /locus_tag="PKU75_00755"
     ncRNA           4214..4597
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKU75_00755"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4663..5319
                     /locus_tag="PKU75_00760"
     CDS             4663..5319
                     /locus_tag="PKU75_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF79433.1"
                     /translation="MKLTHLGHACVLVDMADTRILIDPGEFSDDFTAVRDLDAVLITH
                     QHPDHYDKERLPDLMRANPAARLICDPQTLQKIADLGLDATTHEAAGERIGQVEVTPV
                     GTKHAQIHEDLPIVDNVGVRLAAAGEPVLYHPGDTLAEDPGQIDLLLFPLTAPWQKSA
                     EMVTFLRRLAPRRAVPIHDAIVSAAGRGIYVGHARNLGPQETEVLDLLGAGEQVISRE
                     "
     gene            5316..>5817
                     /locus_tag="PKU75_00765"
     CDS             5316..>5817
                     /locus_tag="PKU75_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79434.1"
                     /translation="MSAAAPTLLTGLLPRRGRVLLIGAGTEALGVSLALRGHHCVGVL
                     PEPEAAALARDAEPLLRWVEGDADSLLLPGRAFDLAVLGEEAVLARSRRTLVPALARV
                     AWHLGASGRLAAGFAPTSTAYGDALPLRDYDRACAAADLAYEARYADWSAARFVAGGC
                     FAVSVHR"
     assembly_gap    5818..5917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6053..6256)
                     /locus_tag="PKU75_00770"
     CDS             complement(6053..6256)
                     /locus_tag="PKU75_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminopeptidase"
                     /protein_id="HPF79435.1"
                     /translation="MAFWYNVVTGAVENDDNKSQGDDVMGPYETEEEASQALALAAER
                     TKAWDDEDKEWEEKGAAATGDSW"
     gene            6312..7175
                     /gene="map"
                     /locus_tag="PKU75_00775"
     CDS             6312..7175
                     /gene="map"
                     /locus_tag="PKU75_00775"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPF79436.1"
                     /translation="MPLTYASVAPGVVSSRRAVPASIARPEYVDRPAPAPYRGPEVKD
                     AETIAKMRVAGRIAAQAMAAAADAIAPGVTTDELDVIAHEFLLDHGAYPSTLGYRAFP
                     KSICTSVNEVVCHGIPDSRRLEDGDIVNIDITAFIGGVHGDTDATYLVGEVDEESRLL
                     VERTHEAMMRGIKAAVAGRPINVIGRVIESYAKRFGYGVVRDFTGHGIGTAFHSGLII
                     PHYDAAPAYGTVIEPGMTFTIEPMLNLGTYEWEMWDDKWTVVTKDRRRSAQFEHTILV
                     TDHGNEILTLP"
     gene            complement(7263..8132)
                     /gene="panB"
                     /locus_tag="PKU75_00780"
     CDS             complement(7263..8132)
                     /gene="panB"
                     /locus_tag="PKU75_00780"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPF79437.1"
                     /translation="MSETPAPAPETTAPYGTGAQAAPQRRVRIPHLLTMKEKGEKWAM
                     LTAYDMYSAEIFDEAGIPVLLVGDSAGNNVYGFETTVPVTVDHLVPLCRAVTSAAKRA
                     MVVADLPFGSYQSSPEQALATATRFMKEGLAHAVKLEGGAPMVPEVELLVRAGIPVMG
                     HIGFTPQSEHVLGGYKVQGRGAGAEKLMDDALALQDAGCFAIVMEMVPAPAAAEVTKA
                     LRIPTVGIGAGPDCDAQVLVWQDMAGLRGGRAPRFVKKYADLRTTLGDAAKAYAADVA
                     AGTFPAEEHSFLE"
     gene            8509..10698
                     /locus_tag="PKU75_00785"
     CDS             8509..10698
                     /locus_tag="PKU75_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HPF79438.1"
                     /translation="MKTRRGGDSLPNPLADAQSKLREEAVAKLLSGEAKLEGSGASRT
                     IKIPGNTSPQARGTDAAKGRWVWYPVNREESIFSILTDFGSKTMAATGGTPGPLHNQI
                     PEPDRNWDGSATDDNSTYWKPDFNRSHYLNMMFGPAPSFRDFYQKQSQGRFLAKGDVS
                     NWVKVPYNQARYGSNEISEADGYWNYIKDTATAWFNSQKAMGKTDAQIKTYLRQFDKV
                     DRYDYDGDGVYLEPDGYIDHFQAIHAGEGEEAGASPDAIWSHRWYAFSTNYGVTGPSF
                     NKAGGVPLGNSGIWIGDYTTEPENGGLGVFTHEFGHDLGLRDYYDTADGTGMGNSTAF
                     WSLMSSGSWLNDGNPDIGTKPGYMGAHEKLMMGWLDYKLVQYGQNTDVKLGIANKDAL
                     HMPQAVAVLLPKRTHIENYNKPYAGNYEWWSGSGDDLQNTMSRALDLTGASTASLNMM
                     MWAEIEEDYDYLYAEVSTDGGSTWKSVWSYTGVNPEWVPVSINLNAYAGKKVDLRFRY
                     GTDGGLAEAGAFLDNIVVKKGSTTVLSDGAENGDNGWTLKGFKRTTGTEKVQSTNYYL
                     AENRIYNGYDHTLRDGPYNFGWANTRPNWVERFPFQNGMLVWYVDNYYADNNTYTHPG
                     EGFALPVDVHPELMAIAGSPLTNRRSSYDATFGVEKTDVVTLHKNGVAKTWGAKMGVR
                     TFDDTNPNRYWSAGNPQNSVKVAGTGTKITVKKSLSRGWVLDVAVRF"
     gene            10890..12227
                     /locus_tag="PKU75_00790"
     CDS             10890..12227
                     /locus_tag="PKU75_00790"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPF79439.1"
                     /translation="MDRQQEFVLRTIEERDIRFVRLWFTDVLGTLKSVAIAPAELEGA
                     FAEGIGFDGSVIEGFARVFESDMLAKPDPATFQVLPWRGDSPGTARMFCDITLPDGTP
                     SIADPRNVLKRALAKCSDMGYTFYTHPEIEFFLLKRGYEPGVKPEPIDNAGYFDHVHT
                     GAAQDFRRTAISMLESVGISVEFSHHEGAPGQNEIDLRYADALTTSDNIMTFRTILKE
                     VAYERDIYATFMPKVFADHPGSGMHTHMSLFDGDTNAFFEAGADYQLSAAARQFIAGI
                     LHHGAEITAVTNQWVNSYKRLWGGGEAPAHLTWGHNNTSAMVRVPMYKPDKGNSTRIE
                     VRTIDPACNPYLAYAVLLAAGLKGIEEGYELPPEAEEDVWSLTQGERQALGIAPLPGS
                     LHDAITIMERSDLVAETLGEHVFDFFLRNKQQEWADYRRQVTEFELARYLDRL"
     gene            12324..15359
                     /locus_tag="PKU75_00795"
     CDS             12324..15359
                     /locus_tag="PKU75_00795"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HPF79440.1"
                     /translation="MRERTESATAALARLGFADPGNAERLVADPALTGLIDPLDTDFD
                     DGVVEALGDVADPDLALLTLVRLMEALRTRIGSNPDDESLGKELPALIATLRRPGVGR
                     DRLLAVIGASIALGDHLVAHPEHWRSVAEARPRTPEQRVARIVGRVRNPDPGLSPDDA
                     LRVYYRRGLLGISALDLVAPDPLVKLPDTAAALADLAAAALEGALVIAQSELPDDAAQ
                     CRFAVIAMGKTGGRELNYISDVDVIFVAEPADGVSEQDAMAAAARLATRLMTACSAAT
                     AHGTLWPVDAALRPEGKAGPLVRTVASHKQYYERWAKTWEFQALLKARPVAGDKEVGA
                     AYLQAMAPMVWQAASRENFVDDVQAMRRRVEAHIPSAEADRQLKLGPGGLRDIEFSVQ
                     LLQLVHGRTDERIRSGTTLDALAALAAYGYVGRDDAAQLAAAYRLLRTLEHRIQLLRL
                     RRTHLMPTSPHELRRLGRALGHREDPAAAVVAEWKAEAREVRRLHERLFYRPLLGAVA
                     RLGTDDARLTPEAARERLSALGFRDPTGALRHLEALTEGLSRRAAIQRHLLPVMLGWF
                     AAEADPDGGLLAFRKVSESLDSSHWYLRLLRDEPAAAEQLARTLARSKFAAEVLVAAP
                     EAVQILGEPGGVRPHSRGDIERRMRTAAQHKTSLDDAIAAARQVRRLELFRIAVADLV
                     GDLPQPDVGRALSDLTAATIEVALQFVIREEEKRAGRPLVTDLLIVGMGRLGGNELGY
                     ASDADVLFVHDPHIGADEDAAQAQALAVVKSMQRVLSVPGPDPAIGLDADLRPEGKNG
                     PLVRSLDSYRAYYDRWALIWEAQALVRARPIAGKAELAARFEDLIDPIRWHGAGLSAQ
                     DLREIRRLKARMEAERLPRGADKRTHFKLGTGGLSDVEWVVQTLQMEHAHNYPTLRTT
                     STLPALTAATQARLISSADSAALAKAWEFAAHARNASMLYRGKAVESVPTDSREADGT
                     ARIMGLPPGSGQEFGEDYRRITRHARAVVDRVFYGREES"
     gene            15414..16751
                     /gene="hisD"
                     /locus_tag="PKU75_00800"
     CDS             15414..16751
                     /gene="hisD"
                     /locus_tag="PKU75_00800"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPF79441.1"
                     /translation="MMQRLDLRGRSLSLRELRESLPRAEFDVDAALHAVRPICEEVRG
                     GGAEAIKELGQRYDGVCPDRLRVPAGALAVALAQLDPDVRAALEVAIERARRVHADQA
                     RTDTTTQVSAGGTVTERWVPVDRVGLYVPGGRAVYPSSVVMNVVPAQIAGVASFAVAS
                     PPQKENTGDLVGYPHPTILAACALLGVEEVYAMGGAQAIAGFGYGFTDGDDVCEPVSL
                     VTGPGNIYVAAAKRLLKGLIGIDAEAGPTEIAVLADATADPEYVAADLISQAEHDPLA
                     ASVLVTDAPDLAEAVAAAVARRAAATKHSERVIEALSGRQSAIVLVDDLDQGLAVVDA
                     YAAEHLEVITADARERAARVRNAGAIFVGPYAPVSLGDYAAGSNHVLPTGGCACHSSG
                     LSVQSFLRGIHVVDYTREALAEVAGHVVTLAQAEDLPAHGEAVTARIPDAAGA"
     gene            16756..17901
                     /locus_tag="PKU75_00805"
     CDS             16756..17901
                     /locus_tag="PKU75_00805"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPF79442.1"
                     /translation="MAEQSPRSGLGALLREDLRGETAYGAPQLSVRAALNTNENSYPV
                     PDEVVADMLAELAAELPGLNRYPDREFTALREALAAYLSRGGTPVEAGQVWAGNGSNE
                     VLLHLLQAFGGTGRRAIGFTPSYSMHSIIATTTATQWIAGKRSDDPARLFTLDPESVA
                     AQIVSADPNIVFICSPNNPTGTAVGLDVVEAAYAAAPGAVVVVDEAYGEFARAGTPSA
                     LTLLAGRERLVVTRTMSKAFALAGGRLGYLAADPGLVDLLRLVRMPYHLSTQSQAVAR
                     AALRHADLMLSTVGVVKEQRDRLVTELTRLGYAPVPSDANFVLFGGLADAAVTWQALL
                     DRGVLVRDVGIPHYLRVTAGTPEETTDFLDVLAALGPEHLLDRGEAN"
     gene            17903..18502
                     /gene="hisB"
                     /locus_tag="PKU75_00810"
     CDS             17903..18502
                     /gene="hisB"
                     /locus_tag="PKU75_00810"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPF79443.1"
                     /translation="MARKATRARRTSESNVRVVVNLDGTGTSRITTGIGFYDHMLGAL
                     AKHALIDLEVEATGDLHIDAHHTVEDVAIVLGEALGEALGDKQGIARFGDATVPLDEA
                     LVQAVVDVAGRPYCVHTGEPEGQAYVVIGQQYVGALTRHFFETVAFRAQIALHVRVLS
                     GRDPHHLVEAQFKAFARALRAAIAPDPRVTGVPSTKGKL"
     gene            18509..19165
                     /locus_tag="PKU75_00815"
     CDS             18509..19165
                     /locus_tag="PKU75_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79444.1"
                     /translation="MPDSDAIAAGALVGNLLLVRARAGQVGAWVGKGLVAAHVTPLGD
                     WTAVTPARVQANTAPPYDDARTTLAARPVSTALRPAIGFFVHGRRGIVVVQPQGWRAV
                     KRWLVWTPEDGPVRAPRLTNARPADLATVAGIGASGIPAVVAAVKSAHAAPIPWLAGL
                     HAALHLPGQSLLTEPGSPRGALVEPSPKRVAAFDHLAREEAAHRAEVVDEPQNPGEQQ
                     "
     gene            19162..19806
                     /gene="hisH"
                     /locus_tag="PKU75_00820"
     CDS             19162..19806
                     /gene="hisH"
                     /locus_tag="PKU75_00820"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPF79445.1"
                     /translation="MSRNNVVVLDYGSGNVRSAVRALERVGASVELTADPAAALACDG
                     LFVPGVGNFHACMAGLRGADGPRIIDVRLSGGRPVLGVCVGMQVLFDGSSEPSAAPVE
                     GIGEWPGLVQRLPAPVVPHMGWNTVSAPAGSLLFDGVADQSFYFVHSYAALTWDLPVA
                     DGHLAAATPLVTWCDYGAPFVAAVENGPLFATQFHPEKSGDAGLHLLENWVRAL"
     gene            19832..20569
                     /gene="priA"
                     /locus_tag="PKU75_00825"
     CDS             19832..20569
                     /gene="priA"
                     /locus_tag="PKU75_00825"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HPF79446.1"
                     /translation="MTDLPRLELLPAVDVRDGRAVQLQQGVAGSGWEFGDPVEAALAW
                     QEQGAEWIHLVDLDAAFGTGSNADLLAEIVGRLDIKVEMSGGIRDEETLARALATGCR
                     RVNVGTAALEHPEWTRQAIASYGDQVAIGLDVRGETLAARGWTRDGGNLWETLARLDA
                     EGCSRYVVTDVNKDGMLAGPNLDLLKAVGQHTTAPLVASGGVSTLADIAAIRQLVASG
                     VEGAIIGSALYKGAFTLPEALDVAGRP"
     gene            20592..21356
                     /locus_tag="PKU75_00830"
     CDS             20592..21356
                     /locus_tag="PKU75_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HPF79447.1"
                     /translation="MSNPRDHAHHPHRVAPGDATDTAGVRWARRDLTPSGFETDMGDS
                     DPALVAALAGADEEAMMAAVAAGRFLVAVVAHAEETVTGADGLVRDASVDMALITLVG
                     PDGRRALPAFTGLAELAAWDPSARPVPVTADRLGQAAVAESCDVIVLNLGAPGERELR
                     SSQVWALAMRRGWTTPHTDPFVAAAVETGLAGSPDVQAHALYAAQPPGTLGVQLTLRP
                     GMTQAQVNGLLTSVGERLAADGEFRARIDGLAFRLG"
     gene            21732..22334
                     /gene="infC"
                     /locus_tag="PKU75_00835"
     CDS             21732..22334
                     /gene="infC"
                     /locus_tag="PKU75_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPF79448.1"
                     /translation="MRLVGPNGEQVGIVRVEDALRLAAEADLDLVEVAPMAKPPVAKL
                     MDYGKYKYEAAMKAREARKNQVNTVIKEIKLRPKIDDHDYGTKKGHVERFLKAGDKVK
                     VTIMFRGREQSRPELGFRLLQRLAADIADLGFVESSPKQDGRNMVMVIGPTKKKSEAR
                     AEQRRRQAEHDVPEATSEAEAPASAPAVSDTDVTDTPEAS"
     gene            22415..22609
                     /gene="rpmI"
                     /locus_tag="PKU75_00840"
     CDS             22415..22609
                     /gene="rpmI"
                     /locus_tag="PKU75_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPF79449.1"
                     /translation="MPKNKTHSGAKKRFRITGSGKIMRQRANHVHKFHEKSPQHARRL
                     ANDVEIAPADVKQVKRLLGR"
     gene            22661..23044
                     /gene="rplT"
                     /locus_tag="PKU75_00845"
     CDS             22661..23044
                     /gene="rplT"
                     /locus_tag="PKU75_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPF79450.1"
                     /translation="MARVKRAVNAHKKRRVVLERASGYRGQRGRLYRKAKEQLNHSLT
                     YSYRDRRAKKGDFRRLWIQRINAGARANGMTYNRFIQGLRAAEIEVDRRMLAELAVND
                     EAAFAALVEIAKANVPATGDEKASA"
     gene            23110..23880
                     /locus_tag="PKU75_00850"
     CDS             23110..23880
                     /locus_tag="PKU75_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPF79451.1"
                     /translation="MRAVAALARRSVRAGSGRFLVEGPQGVRELVRYAAPSVVDLYVT
                     AAAAQRYAALVESASAAGVFVHSVSDEVLAAMADAETPQGMLAVAQQPEPTLADVLAA
                     SPRLLVLLQEVRDPGNLGTVIRAADASGADAVLVTDSSVDVFSPKVVRSTAGSLFHLP
                     VVTGLDAEATLAEVASHGIRTLATEGEAALTLPHVDLAGRHCWVLGTEAQGLPDHLAA
                     ACAERVSIPIYGKAESLNLAMAATICLYASAAAQHGTQ"
     gene            23933..24331
                     /locus_tag="PKU75_00855"
     CDS             23933..24331
                     /locus_tag="PKU75_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79452.1"
                     /translation="MQIDIVEFVGYLLILGVLAALILSASTSARAARLESFRRRQGVH
                     LTEATAPLVSKALSRSRFWRRVGVLVTLLSGVAVTLPIGTWVRGDPATSADWLALLAV
                     GGWEAGTQPTSRGSQASTAMQLPHPSRAHS"
     gene            24328..24693
                     /locus_tag="PKU75_00860"
     CDS             24328..24693
                     /locus_tag="PKU75_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPF79453.1"
                     /translation="MNLEVDLASPTPPYEQVRSQLAGMIAAGQLAAGHRLPAIRQLAS
                     DLGLAPGTIGRVYKELEAEGLVASRVRTGTVVLAAAAVPAARNQQQLAVAARGYASTA
                     RHLGLDLDAAISRLRAEWI"
     gene            24761..26161
                     /locus_tag="PKU75_00865"
     CDS             24761..26161
                     /locus_tag="PKU75_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPF79454.1"
                     /translation="MTHHNAEPIGWDRHAAAVGRVRAQLAALPSGAPVRLAKRTSNLF
                     RPREDTGAQGLDVSCLAGVVEVDPASRVAQVQGMCTYETLVDATLAHGLMPYVVPQLK
                     TITLGGAVTGLGIESSSFRNGLPHESVRELDILTGSGEILTATDDNAHRDLFRAFPNS
                     YGSLGYSVRLMIDLEPVQPHVRLRHVRFTSVPSLVDTLEKILATREFEGHHVDFIDGV
                     VFSADESYLVIAHFTDSTPPYTSDYTGRRIYYRSIQSWHEDFLTIRDYIWRWDTDWFW
                     CSRGLFVQKPLVRRVIPKRYLRSDVYGKVIRFENKHGWYAALQQRRGALPQERVVQDV
                     EIPIERTAEFVDWFLTNVPIEPIWLCPIRLRDTSHGVRPWPHYPLEAGRDYVNVGFWS
                     AVPIVPSAADGDVNKAIEHKVHALDGHKSLYSDAYYDQDEFWSLYGGEEYARVKKLYD
                     PDGRLPDLYAKAVGRR"
     gene            26158..27435
                     /locus_tag="PKU75_00870"
     CDS             26158..27435
                     /locus_tag="PKU75_00870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HPF79455.1"
                     /translation="MTMTIGAIVDEIFGHDAAFGLKAYDGSSGGNQSAGILVDLKNER
                     GLRYLVSAPGELGLVRAYVQGDLDIVGLDEGDPYDLLDSVKDKLAPRIPEPRKVKDIL
                     TSLGPRMFVPPELPDLENPPGWRRLAEGLRHSKARDAQAISHHYDVSNAFYEKVLGPS
                     MAYTCACYPRDDATLEEAQANKFDLVCRKLDLKPGMRLLDVGCGWGGMVRHAVTNYGV
                     TALGVTLSREQAAYGQAWIEREGLTGRAEIRHDDYRHVTESDFDAISSIGLTEHIGIK
                     NYPGYFAFLHERLVAGGRLLNHCITRPNNHFSPIAKGGFTNRYVFPDGELAPVGTIIN
                     DIADQGFEVRHVEDLREHYARTCRAWAKNLSANWDACVGDAGQATARVWGLYLAGSSL
                     GFRRNEIQLHQVLAQKVGSGGVAPYPLRPDFGS"
     assembly_gap    27529..27628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27629..28337
                     /locus_tag="PKU75_00875"
     CDS             <27629..28337
                     /locus_tag="PKU75_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79456.1"
                     /translation="RVTADEDVHVVGGARPATPGRRRWGRAKIAASIGALVGGLLLVP
                     PVRATVLDWISLGGVRVQTPTTSATGSPGTSTPQSVSPRVTATSPVDEGESLSALAQA
                     RTRVGLDFAVPASLGGPTSIMVLHQGKVVEMTWGTGDSALIMDVLTGSLDYGFLKSAR
                     DVEWTDVRGNSALWLPGDHDLQWIDRSGATQTAPPRTARPTLVWVVPKDGADITYRLE
                     GAATKQAALALAAEVGP"
     gene            28428..29213
                     /locus_tag="PKU75_00880"
     CDS             28428..29213
                     /locus_tag="PKU75_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79457.1"
                     /translation="MMQTPMPLTPNSRSHCPQRPIGRLSILGLLLALLTLVGIGPAHA
                     GGPTSVLATNYTDGRAGAAITGTAIYDELVREIGVTGISGGQAPTSQTTQPAEVSDVP
                     AIRLTWFIHDNQPWRIDGIHEAGQNIWISTRESFDGGDLYEVKPMWHKAAHPKKLRSL
                     LIGMGVMVGDANESTSATPAAAPPSPAAVAPPADSGSAAATAAADSSASATPATPETG
                     TRLAWLAGAGALTALALGFLAGRRTIRRDAGPDQGDSAVVWNT"
     gene            complement(29242..30033)
                     /locus_tag="PKU75_00885"
     CDS             complement(29242..30033)
                     /locus_tag="PKU75_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPF79458.1"
                     /translation="MLPTTGYARAAGPLAIAHRGGAALAPENTLAAFARATALGFRYL
                     ETDVRLTRDGELVCFHDERLDRVTEASGPVGRFSLRQLQELRVGGEPICTLREALAAF
                     PAANFTVDLKDQAAIAPLAGVLADPGIAQRVCVAGAWDGWLGQLRDLAPHVHTALGWR
                     SLTGLISSSRTRLPIRWRGAPARYAHVPSRLGRVAIFADGLVARAHAAGVQVIVWTID
                     DPATMHRLLDVGVDGIITDRPDLLREVFIARGLWEPMAAEPISVG"
     gene            30371..31465
                     /gene="pheS"
                     /locus_tag="PKU75_00890"
     CDS             30371..31465
                     /gene="pheS"
                     /locus_tag="PKU75_00890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPF79459.1"
                     /translation="MSGPNTNYDPVEVAALAPEAVETARDAALAAIAAAASLEDLKAV
                     RSAHLGDKSPLALANREIGALPPSAKAAAGKLVGQAKGVVNQAVAARQSELETERDER
                     ILIEEAVDVTLPAARRPLGRRHPIELMSERIADMFVGMGWEIAEGPEVEAEWFNFDAL
                     NFDADHPARQMQDTFYVEPPDGGLVLRTHTSPVQARSLLERGVPIAIACIGRVFRTDE
                     LDATHMPAFHQVEGLCVDEGITMAHLKGTLDTLASHLFGDGITTRLRPSFFPFTEPSA
                     EMDLRCFACRGEDPNCRACKGTGWIEWGGCGMVNRNVLSACGIDPDRYSGFAFGMGID
                     RALMFRTGVSDMRDMIEGDVRFDAQFGMEI"
     gene            31465..34014
                     /gene="pheT"
                     /locus_tag="PKU75_00895"
     CDS             31465..34014
                     /gene="pheT"
                     /locus_tag="PKU75_00895"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPF79460.1"
                     /translation="MRVPVDWLREYVAVPADATGVQIAADLVKVGLEEEGLHTSGVTG
                     PLVVGRVLTKEPEPQKNGKTINWCSLDVGDANGTGEPQWVVCGAHNFEPGDLVVVILP
                     GGSVSTPQGPMVVTARKTYGHISSGMITSERELGIGEDHSGIIVLSRFFADRPEVLAG
                     LAPGQDAIPLLGLDRETVEVNVTPDRGYCFSIRGIAREYSHATGAAYTDPVLALADRA
                     PAANEQGYAVQLADDAPIRGREGCDRYVARIVRGVDVHAPSPAWMKARLTEAGMRPIS
                     LPVDVTNYVMLGLGQPLHAFDLAKLVGPIVVRRARPGERMTTLDDVDRALDPSDLLIT
                     DSESQRALAIAGVMGGADTEISDTTTDVLIEAAHFDPVSIARSARRHKLPSEASRRFE
                     RGVDPQLAAAAAQLAVELLVEHGGGVVDDGVTDVGSPVPAPDIDLDLSLPSRLVGVDY
                     PSERVVEILREIGCGVEVSGEHARVTPPSWRPDLRTEPDLAEEVARIDGYEKIPSVVP
                     TVAVGNGLTHDQRMRRLVANLLAAQGFSEVWNAPFVGDDRHVALGYDAAAARARTVRI
                     ANPLSDEAPLMRISILSTLVDSLRRNISRGFKDVALFEQGLVLALEGKQTAAPTEDVG
                     VRPSPQTMAAIHAAVPPQPRHLGFVLAGEIDRSGVWGAGRPADVADAIATALGITEAL
                     GLTARTVADDAMPFHPGRCARVELADGTVVGHVGELHPKVLTALGLPARVVAGELDLD
                     ALSAASGRPAQAVTLSTFPVATTDVALVVDESVKAADIEAALRTGAGEMLESVRLFDI
                     YRGDQTGAGKKSLAYRLSFRAPDRTLTTEEVSGIRDQAVAAAGQAVGAVQR"
     gene            34142..35185
                     /gene="argC"
                     /locus_tag="PKU75_00900"
     CDS             34142..35185
                     /gene="argC"
                     /locus_tag="PKU75_00900"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPF79461.1"
                     /translation="MSVRVAVAGASGYAGGELVRLLLAHPEVEIGALTASSNAGQTLG
                     SIHPHLTPLRDRLLEDTTAEVLAGHDVVFLALPHGHSAALAAQLPDEVVLVDCGADFR
                     LESEGDWTTFYDTPYAGSWPYGLPELPLAEGNQRDLLAGAKRIAVPGCYPTGISLALA
                     PGLRAGLLDPADIVIVAASGTSGAGKSRKPHLLGSEVMGAMSPYGVGGRHRHTPEIEQ
                     NLSRAAGAPASVSFTPTLAPMARGILATSTARLTAGASAGQLREAYAAAYEREPCVVL
                     LQDGVWPRTSDVLGANTVHLQVTVDPRQGRLIAVSAIDNLTKGTAGAAVQCLNLALGL
                     PELTGLSLVGVAP"
     gene            35208..36359
                     /gene="argJ"
                     /locus_tag="PKU75_00905"
     CDS             35208..36359
                     /gene="argJ"
                     /locus_tag="PKU75_00905"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPF79462.1"
                     /translation="MSVTTPTGFRAAGITAGLKASGRPDLALVVNDGPDRCAAAVFTT
                     NRVQAAPVIWSRQVLADQRVDAVILNSGGANACTGPQGFQDTHKTAEVVAELLGISAG
                     DVAVCSTGMIGELLPMDTLLAGCAQVAGELSGDGGPAAAEAIMTTDTVAKTAAYSGPG
                     WSVGGMAKGAGMLAPALATMLVVLTTDAQVAPADLDAALRAATAKTFDRIDSDGCMST
                     NDTVIALASGASAVPVAPADLEAALTEVCTDLALQLIRDAEGAAHDIRIEIDSAATES
                     DALEVGRAIARSNLFKCAIFGNDPNWGRILAAAGTTSAAFDPATIDVSINGVIVCRDS
                     GVGDDRALVDLTGRDVHIVVDLKAGEESATIWTNDLTHDYVHENSAYST"
     gene            36373..37329
                     /gene="argB"
                     /locus_tag="PKU75_00910"
     CDS             36373..37329
                     /gene="argB"
                     /locus_tag="PKU75_00910"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPF79463.1"
                     /translation="MTGTTLRATPDGAAIAAAQAKAATLTEALPWLERFRDALVVVKY
                     GGNAMTDRALQEAFAEDVAFLRYAGLRVVVVHGGGPQIKSMLGRLGMSSEFKGGLRVT
                     TPEVMDVVRMVLTGQVGRELVGLLNQHGPIAVGLSGEDAGLFGARRRGVVVDGIEEDI
                     GLVGEVVTVDPSAVHDIIAAGRIPVVSTIAPDIEVEGQVLNVNADTAAAALAAALGAR
                     KLVVLTDVEGVYAAWPDRSSLLSAMDLTAAKDLLTRVDEGMIPKLEACITAVEKGVPQ
                     AHVIDGRQPHSLLLEVFTSEGIGTMITRDDALDGPVCQGDPL"
     gene            37326..38549
                     /locus_tag="PKU75_00915"
     CDS             37326..38549
                     /locus_tag="PKU75_00915"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HPF79464.1"
                     /translation="MTAESPQPAPPTRSEQLLDRYEEALLGVFGRPQLVIERGEGCYI
                     WDADGKRYLDLLAGIAVNALGHNHPELVAAVSKQVGEVAHISNFFTSGAQIELAERLL
                     AIAGAPEGSRVFFTNSGTEALEGAVKLARRTGRTGIIASEGAFHGRSTGALALTAKPA
                     YREPFEPLIPGVSHIPYGDADAVEDALAAGDISAVVLEPIQGEGGVIVPPDDYLTRVR
                     ELTHAARALLILDEVQTGIGRTGDWFAFQRSGIQPDAITIAKGLGGGIPVGALVTFGP
                     EVSGLLQPGMHGSTFGGNPLASAAGLAVLSTIERDGLLRHTTEVGDGFAAAVLALGHP
                     QIREVRGRGLLRGIALSSPIAPAVMGIARADGFIVNAVAPDAIRIAPPLIITAEQLAS
                     FTAALPGILDRAVNS"
     gene            38568..39551
                     /gene="argF"
                     /locus_tag="PKU75_00920"
     CDS             38568..39551
                     /gene="argF"
                     /locus_tag="PKU75_00920"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPF79465.1"
                     /translation="MSDNVSIPAETVRHFLADDDLSPQEQAEVLARAAALKAAPFSDR
                     SLEGPQVVSILFDKPTLRTQVSFVGAVAHLGGHPMTVDANLAKVGERESVADVARVLG
                     RQSAVIVWRTYGQDRIEEMARYAGVPVVNALTDDYHPCQILADLLTMTEHKGTLAGLT
                     VGYVGDGGNNMAHSYLLGCALAGMHIRIGTPAAHQPKAEIVERAVAIAAQTGGSVLVT
                     DDPIAAVHGADVVITDTWVSMGMEGEKAERQGEHSPFEKYSVTPELMGLADPAAIFLH
                     CLPAYRGYEVDAAVIDGPQSVVWDEAENRLHAQKAVISFLLEKSGHAAGAV"
     gene            39548..40051
                     /locus_tag="PKU75_00925"
     CDS             39548..40051
                     /locus_tag="PKU75_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851441.1"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HPF79466.1"
                     /translation="MTVASSRGARQQRIVDLLGEHAVRSQTQLLELLAAEGIEVTQAT
                     LSRDLVELGAEKVRVGKSLIYAVPGEGGDRTVRPAPEAAEVSRRLASRCQELLVSAAC
                     SANLVVLRTPPGAASFLASAIDHGQVDGVLGSIAGDDTIMLITEGAAASESVAAHLLS
                     LSGKESA"
     gene            40048..41457
                     /gene="argH"
                     /locus_tag="PKU75_00930"
     CDS             40048..41457
                     /gene="argH"
                     /locus_tag="PKU75_00930"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPF79467.1"
                     /translation="MTEIVSLWGGRFSGGPADALAALSKSTHFDWRLARQDIAGSRAH
                     AQVLAAAGLLDEPTLHAMLTGLDELESDVLAGTFTPAEDDEDVHTALERGLIERAGAD
                     VGGRLRAGRSRNDQIATLFRMYLREQARHLAGRVLDVVDALLAQAERHHGVAMPGRTH
                     LQHAQPVLLSHHLLAHVWALLRDVDRFGDWDRRADASPYGSGALAGSSLGLDPEKVAA
                     ELGFARSVENSIDGTAARDFAAEFAFVCAMTAVDISRIAEEVVLWATKEFGFIRLEDA
                     YSTGSSIMPQKKNPDVAELARGKAGRLVGDLAGLLTTLKALPLAYNRDLQEDKEPVFD
                     AIDTLDVLLPAFAGMVATMQFDTERLESLAPQGFSLATDIAEWLVRQGVPFRIAHEVA
                     GGCVRTCEERGIELWDLSDDDLAAISPHLTSGVREVLSVQGSLASRDAKGGTAPVRVA
                     EQLTAARGAALRAREFATG"
     gene            41464..42105
                     /locus_tag="PKU75_00935"
     CDS             41464..42105
                     /locus_tag="PKU75_00935"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPF79468.1"
                     /translation="MTAARLPRSFFARDVLDVAPALLGAHLVHGGVTLRLTEVEAYAG
                     EKGDPGSHAFRGQTPRTAVMFGPPGHLYVYFTYGMHYCANVVTGADGLASAVLLRAGE
                     VIAGHELAAARRPGIPERDWARGPARLTVTMGLDRAHNGDDLCGRAAEPRIEPASAAI
                     DPARIRTGPRVGVSGPGGDGTAYPWRFWLDGEPTVSAYRPGVVRTRRPNAKKA"
     gene            complement(42115..42609)
                     /locus_tag="PKU75_00940"
     CDS             complement(42115..42609)
                     /locus_tag="PKU75_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79469.1"
                     /translation="MPRLREMTEADVADFAPLFREIVAAGETYAYPEGLSDAQIAALW
                     LERPPARTIVAEDEDATLLGSAKMGPNRPGRGAHIGTASFMVAAAARGRGIGRLLAEE
                     MIDWHRREGYAGIQFNAVVETNTAAVRLWQDLGFTIVGTVPAAFESASRGRVGLHVMF
                     LDLT"
     gene            complement(42593..43612)
                     /locus_tag="PKU75_00945"
     CDS             complement(42593..43612)
                     /locus_tag="PKU75_00945"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPF79470.1"
                     /translation="MLPSTANFIAGLPKAELHVHHVGSARPETVAELAARHPDSPVPK
                     DLDGLRRFFTFTDFAHFIEVYLSVVDLLRTPEDLRLLTYEVIEGLAAQQVRYAELTMT
                     PYTSVVRGIPIEAYTEAIEDARVAAERDHGTVVRWVYDIPGESGVPAADATIGYAVDH
                     APDALVAFGLGGPEIGVPRAQFAPHFARAQAAGLHSVPHAGESTGPETIWSAIRDLGA
                     ERIGHGVRAVDDPELVAYLVQHGIPLEISPTSNLATRVVERIEDHPVRALRDAGVTVT
                     INSDDPPMFGTTLNREYALAAEVLGLDAAGLAALARTAVDVSFAPEEVKADVREQIDG
                     YAASA"
     gene            complement(43624..44529)
                     /locus_tag="PKU75_00950"
     CDS             complement(43624..44529)
                     /locus_tag="PKU75_00950"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPF79471.1"
                     /translation="MHENEMRADAALVAALIGQQAPQWAELAVTPLPAEVEGTDHVLF
                     RVGPDLLARMPKIDWAVEQGAQEAAWLPVLAPHVPVALGVPVFAGRPGHGYPWPWTIV
                     RWIPGQTPPRLGLDDPGLARQVAAFARALHAVPAQGGPLCPPGSRGTAPRVQAERGRA
                     AIDGLRGKHDGLDLDAVARAWEQVVATPDYAGEPVWLHGDLQPGNLIVADGRLSGVID
                     FTPRRGDPAADLAPAWWTFTGDARTLYREELADYGPEAWWRARGWALLPSLTGIDYYR
                     NTFPRMAEHGRRTVTAVLQDIERFG"
     gene            44598..44951
                     /locus_tag="PKU75_00955"
     CDS             44598..44951
                     /locus_tag="PKU75_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HPF79472.1"
                     /translation="MAYDEVLAERIRGAISGVEGVQEMAMFGGLGFLVGGHMAVAAAG
                     KGWLMARVDPAETDSLITRPGVEPFEMRGRGMTGWLLVAEDALDDEADLREWVDRGVA
                     YVATLPPKPRKAKKG"
     gene            complement(44948..45412)
                     /locus_tag="PKU75_00960"
     CDS             complement(44948..45412)
                     /locus_tag="PKU75_00960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79473.1"
                     /translation="MATRPTIRRAQTADTDTVTRHRDAMFTEMGIDAGVVAAASGPAR
                     TWLAAALADGRYVGLLAESGGAVIGGIGITWLDLPPNMNTQLDRRGYLLNMFVEPVAR
                     SQGVARALLRAALDECRAQGVDCVSLHASDAGRSLYESSGFSPTDEMRLRLA"
     gene            complement(45513..46535)
                     /locus_tag="PKU75_00965"
     CDS             complement(45513..46535)
                     /locus_tag="PKU75_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF79474.1"
                     /translation="MRSLRWALLGASDIAATRMIPAIESTGGRAVTVLSGSPGHAEAF
                     ATSHGIERATTDLGAALEGVDAAYISSHNPKHAEQAQAALEAGVHVLCEKPITLDLGQ
                     AVDLIALADSRGLVFGVNHHLSCSAVIRKLAALVHEGTIGTVTGIRINHAVFLPERLR
                     GWRIEDVPGAGVILDITVHDAAVLGLLRDAPPATAAAQVARQGPWPKGAPDAVMTTLR
                     WTDEVLAQTHDAFTVPFNSSSVDVYGSEGTLHATDALTQDPVGDLTLRDEAGVRAVDV
                     GNREDLYVVSVRDFTAAVEGTGAVAVTGMAGTQAVAVALAVQEAAETGRTVDVVDVRG
                     LLSPAR"
     gene            46825..>47448
                     /gene="tyrS"
                     /locus_tag="PKU75_00970"
     CDS             46825..>47448
                     /gene="tyrS"
                     /locus_tag="PKU75_00970"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPF79475.1"
                     /translation="MSTILDELQWRGAVAQSTDETALREALASGPITVYCGFDPTAPS
                     LHFGNFVQLVSLRRMQRAGHKVICLVGGSTGLIGDPKPTSERVLKTKEQTAENVARIK
                     ELVRPFLDFEGTDPSCAYPAILVDNLDWTAPMSALDFLRDVGKHFRVNQMIKKEAISA
                     RLESQEGISYTEFSYQLLQALDFLELFRAHGCTMQIGGSDQWGNLTAG"
BASE COUNT         7294 a        17104 c        16021 g         6829 t
ORIGIN      
        1 gtttagcccg aactccctgg cacatatctg ccggagtccg ggctaagcca ggcagttcgc
       61 ggcatcttca gccgaggatg ccccggaagt attgctgcag agcggcatcc agagccgtgc
      121 cacccccgat gacataggcc ttgacgccgc cgttgtcctt ggcccagggg gccgtgttgg
      181 cgacggtggc cggcacgagg acggtcccgg aatcggtcgg ggagaggact ccgatggccg
      241 cgtcgtaccg gtcccagcgc atccggccgg ctgcgtcggc gagccgcaga taaacctcgt
      301 cctcaccgcg ccggttggct ggtccccccg cagtcgcgcc gggcgcgtgc gggcccgcgg
      361 ccttgccgta tgccgctgcc gccttggccc cgacggcacc agaccagagc agtcccttgg
      421 cggcgtagac gcagatcccg agcgacgccg gtgccgtcgg acctgccgga gtggagtcac
      481 ggatgacgtc gggtggcgag gcggggcagc cgttggcgtc caccgtgacg gacgaggccg
      541 aaccgaggat ccgctcgcgc agggcggcgt ccttgctgaa gaccgtcacc gtcacctcac
      601 cgactgagcg cgtctcgcgc acgacgccgt cgggatagct ctcggtgccg gccttgaccc
      661 ccgccgcatc gaaccacacc gcgtcgacct tgggatggac atcgggtgac ccaacgcagg
      721 cgtcggtcat catcacggga cggccgacgt aggggacacc gtcgggcacg aactcgtagg
      781 cagaactccc cgggacggtg aaggcgcggc cttcactgca gtcgagcacc tccccgttgc
      841 cccaatccgg agccatcggg gcaccgcccc agccccacgt cggcggaacc cacagctgca
      901 ccccgccata cgactccggc cgccagtcgg cgggtatgcc ggccggtcgc ccgtccgccg
      961 tggcggcggt cttcggtgac tcagaagtcg tcgaggagtc ggcgcctgtg gaggcgctcc
     1021 gggacgcgcc ggccacaggg tcggtgacgc cggaatcgtt gtcccgcaat gtgttcgcca
     1081 cgagaatggc accggggacg gcaagggcgg ccaccgcggc agcggccggg aggacccagc
     1141 gccagcgagg agaggacggc ggaacggcgg ctgcggtcgc gctgcctgaa gaccgcgatc
     1201 cgccggatcc gggcaggggg gcgccgaggt cgctcggcgc gtcgccggcg tggtggcgca
     1261 gtccgtcgcg gatcatccgt tcgaggtcgg cgtcgtcata ggtgtgcttg gtcatggcag
     1321 gtcctcggaa gtcagctgga tgcgcagcgt gatgagcgca cggtggatgt gcgagcgggc
     1381 ggtggcctcg gagatgtcga ggacgcgggc gatctcgcga tagtcgaggt cttcgtagaa
     1441 gcgcagcacg acggcggcgc gttgctgggg cggcaggcca aggcacatcc gccagacggc
     1501 gtcgtgctcg gcgatctgtt cggtgctgtc ggcggcggaa ccgccacgcg gttcagcgac
     1561 gctgaactca cggcgtccga tccggcgcca ctgcgagaca tgcgaattcg cgatcatgcg
     1621 ccgcacgtat tggtcgggtt gcgcccgggt ggatacgtga tcccacgtct cgtatgccgc
     1681 agcgagcgcg ccctgcaccg cgtcctgggc ggcgtcgcgc gagccggtga ccagataggc
     1741 gaagcggagc aggccgggaa gccgaggcgt cacccacgtc tcgaacccgg cgcggtccac
     1801 tccggccttg tccacggtcg tcaccatgcc ctactcacgc cgcagcagcc cgaggtgttg
     1861 catcggcggc gaaacgaaaa ttctcagtgc gggagcacgt ccagggtcca gcctgccccc
     1921 ggcttggccg gctcgtgcaa ctcgatgcgg aagtcgttct ccagcaacgg aatcaatccc
     1981 gcgggtcgcc gggggtccgc gtccgactca cacagcaccc gcgccaccag gccgcgcgtg
     2041 tgcttggcca tatggctcgc ccccggcacg cgcacggcga cccagcgctc ggcgagcgca
     2101 cccgtggggc gccacgtcgc ggcatactcg ccggaccggc agtcgacgat cagccccttg
     2161 ccagccgccg ccgacagcac cgggtcgagc gactccttcc accgatgcgc caacttgccc
     2221 acctcgggca ggtgcgcgca catcgacgcg cgatagggcg cgatccgatc gcccgcccgc
     2281 accgcgccat acaccgccga ggagatcacc agccgccgcg ccgcccgccg ccggccaccg
     2341 ggggagagcg agggggcgtc gagcgcgtca tagagcacgc cttgatagat ctcgagcgcc
     2401 gggcgcgcgg ggcgggtgcg gatggctagg ttgccctcca cctccgcgcc gagcgtcgac
     2461 gggacataga ggatgcgctg ggcgtcctcc agcccggacg cgttgatcac cgcgtcgatg
     2521 acctcctcgc gccgccccgt caactcgggg aaggagagag cgtcgacccg catcggcgcg
     2581 ccgcggctgg gccgggcctt ggtctccgac ggcggcaaca ggatcagcac ggcgtcactg
     2641 tatgaggtga ccgccccgtt aggtttgagg catggcgcaa cccatgcgag cccgctactc
     2701 cgacaccggc atcgccgccg tcctcgccga cgaccgattg ctcgcccgct tcgacgcgat
     2761 ccgagccgag ttcaccgtcc ccgaggcatt cccgccggag gtgctcgccg aggccgacca
     2821 gatcgccggt gccccgatgg agtggcccga gcgcgatgag accgccgtgc cgttcctgac
     2881 gatcgacccg cccggatcga tggacctcga ccaggccgtg cacatcgagc gcgccgaggg
     2941 cggcttccgg gtccgctacg cgatcgccta cctgcccgcc ttcgtcgccc ccggcggcgc
     3001 catcgacggc gaggcgcacc ggcgcggcca gacgatctac gccccggacg agcgcacccc
     3061 gctgcacccg ccccaggtga gcgagaacgc ggcgagcctg ctgccggacc aggtgcgggc
     3121 ggcatacgtc tgggacatgc acgtcaagga cgacggcgaa ggcgaccaga agaccgtcta
     3181 ccgcgcgctg gtgcgcagcg tcgaccggtt cgactacgag caggtgcagg cgatgatcga
     3241 cgccggcacc gacgacgacc gggtggcgct gctgaaggag gtcggcgagc gcaggatcgc
     3301 cttcgagcag cgccgtggcg gcgcgagctt gccgatgccg gaacagcagg tcacgcagga
     3361 cgaggcgggc aagttcgtcc tcgggttccg gccgccgctg cccgccgagg agtggaacgc
     3421 ccagatctcc ctgatgacgg gcatggccgc cgccgaactg atggtcgagg cccggaccgg
     3481 gatcctgcgc accctgcccg cgccggacga gcaggcagtc gagcggttcc gccggcaggc
     3541 caagacgctc ggcgtccagt ggcaggaggg ggagtcgtat ggcgcgttcc tgcgccgcct
     3601 cgatcgcacc aacccccacc acctcgccct gatctatgac gcgacggggc tcttccgcgg
     3661 ctcggggtat gtcgccttcg acggcgaggt gccgccggcg ccggagcacg gggcgctggc
     3721 ctcgacctat gcgcacgtca ccgcgccgct gcggcggctg gtcgatcgct tcgggctggc
     3781 gatcgccgag gcgctggccg ccggccggcc ggtgcccgag tgggtgcgat ccggcatcgg
     3841 cgtgctcccg gagatcatgg cggccaccgg ccgcaccgcg tccggtgtcg aacgcgcctg
     3901 caccgacgcg gtggaggcgg ccgtgctctc ccaatacgtc ggcgatacct tcgaggccgt
     3961 ggtcgtcgac caggggcaga agggggcgtg gctgctgcag ctgaccgacc ctgccgtggt
     4021 cgcgcatgcc ggcaccgagg tcaaagtggc ggtcggcgac ctcgtcgcgg cgcgcctgac
     4081 cgaggccgac gtcacggcag gcgtcgtgcg cttccacatc accgcgcgca agggcgccgt
     4141 ccccgcgccc gcgggagtgc ctgcgtcgcc ggaggacccg ctggcccctc cggcgtagac
     4201 tgggtggcga ggaagagccg gccagacggt cgcgtccggt atgccgcgcg gcataccggc
     4261 cgaggaaggt ccggactcca cagggcaagg tgatggctaa cggccacccg gggcgacccg
     4321 cgggacagtg ccacagagag cagaccgccc caccttcggg tggggtaagg gtgaaagggt
     4381 ggtgtaagag accaccagcg gcctgggcga ccagaccggc tcggtaaacc ccacctggag
     4441 caagcccaga tatgcgtgcg ttcgacggcg gcccgccgga gcacgcgggt agggcgctcg
     4501 agcccgccgg caacggcggg cctagatgga tgaccgtcac cgcacctgcg gcaacgcgga
     4561 tgcggcacag aatccggcct atcggccggc tcttcctcac tcaccgggaa acctgcaaag
     4621 cccgggaccc acgatcgggc aaccacgcct acattggcgg gtatgaagct gacccacctt
     4681 ggacatgcct gcgtcctggt cgacatggcg gacacgcgca tcctgatcga tccgggcgag
     4741 ttctccgacg acttcaccgc cgtacgcgac ctcgacgcgg tgctcatcac ccaccagcac
     4801 cctgatcact acgacaagga gcggctgccg gacctgatgc gcgccaaccc ggccgcgcgc
     4861 ctgatctgtg acccgcagac gctgcagaag atcgccgacc tcggcctgga cgccacgacc
     4921 cacgaggcag cgggggagcg gatcggtcag gtcgaggtca cccctgtcgg caccaaacac
     4981 gcccagatcc acgaggacct gccgatcgtc gacaacgtcg gggtgcgcct ggcggctgcc
     5041 ggtgaacccg tcctctacca ccccggcgac accctcgccg aagatcccgg gcagatcgac
     5101 ctcctcctct tccccctcac ggcgccgtgg cagaagtccg cggagatggt caccttcctg
     5161 cgccgcctgg ccccgcgccg ggccgtgccg atccacgacg cgatcgtctc cgctgccggc
     5221 cgcggcatct atgtcggcca cgcccgcaac ctcggccccc aggagaccga ggtcctcgac
     5281 ctgctcggcg ccggagagca ggtcatcagc cgcgagtgag cgccgccgcc ccgaccctgc
     5341 tgaccggcct gctcccgcgc cgcggcaggg tgctgctcat cggcgcgggc acggaggccc
     5401 tcggggtgtc gctggccctc cgcggccacc actgcgtcgg cgtgctgccc gaacccgagg
     5461 ccgcggcgct ggctcgcgac gccgagccgt tgctgcggtg ggtcgagggc gacgccgact
     5521 ccctgctcct gcccggtcgc gccttcgacc tggcggtgct cggggaggag gccgtgctgg
     5581 cgcgctcgcg ccgcacgctc gtccccgcgc tcgcgcgcgt tgcctggcat ctcggcgcgt
     5641 cgggccggct ggcggcggga ttcgcgccca cgtccacggc atacggtgac gccttgccgc
     5701 tgcgcgacta cgaccgcgcg tgcgccgcag ccgatctggc ctacgaggcg cggtatgccg
     5761 actggtccgc cgcgcgcttc gtcgccggcg ggtgcttcgc cgtcagcgtg caccgccnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc gctgcaagaa gtgcccgatc
     5941 gcgctcaagc ggctcgccga cgccggctat taccggcctg gacaaacgct cagacgaact
     6001 gctcgtgaag cccggcacgg aggcggtcaa gaccgccctg gcgaagtagg gactaccagg
     6061 agtcgccggt ggctgcggcc cccttctcct cccactcctt gtcctcgtcg tcccaggcct
     6121 tggtccgctc ggccgccagc gccaacgcct gcgaggcctc ttcctcggtc tcgtagggac
     6181 ccatcacgtc atcgccctgg gacttgttgt cgtcgttctc gaccgcgccg gtgacgacgt
     6241 tgtaccagaa agccatcggg tgtcctttcg tgtgggccgc acgccggcgt gcggtgctta
     6301 gactccaccg tatgccgttg acctacgcaa gcgtggctcc gggtgtggtc tcttcgcgcc
     6361 gcgccgtgcc cgcctcgatc gcccggccgg agtatgtcga ccggccggcc ccggctccct
     6421 accgcgggcc ggaggtcaag gacgcggaga cgatcgcgaa gatgcgggtc gccggccgca
     6481 tcgcggcgca ggcgatggct gccgcggccg acgcgatcgc gcccggggtg acgaccgacg
     6541 agttggacgt catcgcccac gagttcctgc tcgaccacgg cgcctacccc tcgacgctcg
     6601 gctatcgcgc ctttcccaag tcgatctgca cgtccgtcaa cgaggtggtg tgtcacggca
     6661 tacccgactc gcggcggctc gaggacggcg acatcgtcaa tatcgacatc accgccttca
     6721 tcggtggggt gcacggcgac accgacgcga cctatctggt gggggaggtc gacgaggagt
     6781 cgcggctgct cgtcgagcgc acccacgagg cgatgatgcg ggggatcaag gcggccgttg
     6841 ccgggcgacc gatcaacgtc atcggccgcg tcatcgagtc ctatgccaag cgtttcggct
     6901 acggcgtcgt gcgcgacttc accggccacg gcatcggcac ggccttccac agcggcctga
     6961 tcattcccca ctacgacgcg gctcccgcat acggcaccgt catcgagccc ggcatgacgt
     7021 tcacgatcga gcccatgctc aacctcggaa cttacgagtg ggagatgtgg gacgacaagt
     7081 ggacggtcgt cacgaaggac cggcgccgct ccgcccagtt cgagcacacg atcctcgtca
     7141 ccgaccacgg caacgagatc ctcaccctcc cgtagaggta gaacgttctc tcaacccgtc
     7201 gagggtgcac gacgcgccgt aaaggtcgac tcatgacggc gcgtcgtgcg ctttcgaccg
     7261 ggtcactcga ggaaggagtg ctcctcggcg gggaaggtgc cggcggcgac gtcggcggcg
     7321 taggccttgg cggcatcacc gagggtggtg cgtagatcgg cgtacttctt gacgaaccgg
     7381 ggtgcgcggc cgccgcgcag gccggccatg tcctgccaca cgagcacctg ggcatcgcag
     7441 tcggggccgg cgccgatgcc gaccgtcggg atccgcagcg ccttggtgac ctcggcagcg
     7501 gcgggggccg gcaccatctc catgacgatg gcgaaacagc cggcatcctg cagtgcgagc
     7561 gcgtcgtcca tcagcttctc ggcgccggcg ccgcggcctt gcaccttgta accgccgagc
     7621 acgtgctcgc tctgcggggt gaagccgatg tgccccatga cggggatgcc ggcccggacg
     7681 agcagttcga cctcgggcac catcggcgcc ccgccctcga gcttgacggc gtgggcgagg
     7741 ccctccttca tgaaccgggt ggcggtcgcg agggcctgct cgggggagga ctggtaggag
     7801 ccgaacggca ggtcggcgac gaccatggcg cgcttggcgg ccgaggtgac ggcccggcac
     7861 agcggcacga gatgatcgac cgtcaccggg accgtggtct cgaagccgta gacattgttg
     7921 cccgccgagt cgccaacgag gagaacggga atgcccgcct cgtcgaagat ctccgcggaa
     7981 tacatgtcgt atgccgtgag catcgcccac ttctcgccct tctccttcat cgtgagcaga
     8041 tgagggatcc ggacgcgacg ctgcggtgcg gcctgggcgc cggttccgta gggggcggtg
     8101 gtctcggggg cgggcgccgg ggtttcgctc ataacgccga tcgtatgccg cgcagacacg
     8161 acgcagggcc ccgcccacgg tggtgggcgg ggccggttgt cgccgtcgtt ttcgggccga
     8221 acggtccgcg ttatcaaatc gtgacgtgaa ttctacggtc gtgctgtctt cctgccggaa
     8281 aacgccggct cccgggggta gcacgccagg acctttcgtg cttgcctaac ccccgggtca
     8341 cccgaccggt gatccagacc agcacctggg aggaagtaac acgtgagaaa acgtcacgtt
     8401 ggagctgtgt ccgggatcgc cattgcggcg ctcgccctga ccattgctcc gagcacggcg
     8461 caggccgggc ccagccccgc gcccgcagcg ggctccgcgg atggagccat gaagacccgt
     8521 cggggcggcg acagcctgcc caacccgctc gccgacgcgc agtccaagct gcgtgaggaa
     8581 gcggtcgcca agctgctgtc cggcgaggcc aagctcgaag gcagcggcgc gagccggacc
     8641 atcaagatcc cgggcaacac cagcccgcag gcgcgcggca cggatgcagc gaagggccgc
     8701 tgggtctggt accccgtcaa ccgcgaggag agcatcttct cgatcctgac cgacttcggc
     8761 agcaagacca tggccgccac cggcggaacg cccggtccgt tgcacaacca gatccccgag
     8821 cccgaccgca actgggacgg cagcgccacg gatgacaaca gcacctactg gaagccggac
     8881 ttcaaccgtt cgcactacct gaacatgatg ttcggtccgg ctccgtcctt ccgggacttc
     8941 taccagaagc agtcccaggg ccgcttcctc gccaagggtg acgtctccaa ctgggtcaag
     9001 gtgccctaca accaggcccg ctacggcagc aacgagatca gcgaagccga cggctactgg
     9061 aactacatca aggacaccgc gaccgcctgg ttcaacagcc agaaggccat gggcaagacg
     9121 gacgcccaga tcaagaccta cctgcgccag ttcgacaagg tcgaccgcta cgactacgac
     9181 ggcgacgggg tctacctcga gccggacggt tacatcgacc acttccaggc catccacgcc
     9241 ggcgagggcg aagaggccgg cgcgagcccg gacgccatct ggagccaccg ctggtatgcc
     9301 ttctccacca actatggcgt gaccggcccc agcttcaaca aggccggcgg cgtccccctg
     9361 ggcaactccg gcatctggat cggcgactac accacggagc cggagaacgg cggcctcggc
     9421 gtcttcacgc acgagttcgg ccacgacctc ggcctgcgcg actactacga cacggccgac
     9481 ggcaccggca tgggcaacag cacggcgttc tggtcgctga tgtcctccgg ttcgtggctc
     9541 aacgacggca acccggacat cggcaccaag cccggctaca tgggcgccca cgagaagctc
     9601 atgatgggct ggctcgacta caagctggtg cagtacggcc agaacaccga cgtcaagctc
     9661 ggcatcgcca acaaggacgc cctgcacatg ccgcaggccg tcgcggtgct gctgccgaag
     9721 cgcacgcaca tcgagaacta caacaagccg tatgccggaa actacgagtg gtggagcggc
     9781 tcgggtgacg atctgcagaa caccatgtca cgcgcgctcg acctgaccgg cgcctccacc
     9841 gcatcgctga acatgatgat gtgggccgag atcgaggagg actacgacta cctctacgcc
     9901 gaggtctcca ccgacggtgg ctcgacgtgg aagtcggtct ggagctacac cggtgtcaac
     9961 ccggagtggg tcccggtcag catcaacctc aatgcttacg ccggtaagaa ggtcgacttg
    10021 cgcttccgtt acggcaccga cggcgggctc gccgaggccg gcgccttcct cgacaacatc
    10081 gtcgtcaaga agggctcgac gaccgtgctg tccgacggcg ccgagaacgg cgacaacggc
    10141 tggacgctca agggcttcaa gcgcaccacc ggcaccgaga aggtccagtc gacgaactac
    10201 tacctcgccg agaaccggat ctacaacggc tacgaccaca cgctgcgcga cggcccctac
    10261 aacttcggtt gggccaacac caggccgaac tgggtcgagc gcttcccctt ccagaacggc
    10321 atgctggtct ggtacgtcga caactactac gccgacaaca acacctacac ccacccgggt
    10381 gaaggcttcg cgcttcccgt cgacgtccac cccgagctga tggcgatcgc gggcagcccg
    10441 ctcaccaacc gccgcagctc ctatgacgcc accttcggcg tcgagaagac cgacgtcgtc
    10501 acgctgcaca agaacggcgt ggccaagacg tggggcgcca agatgggtgt tcgcaccttc
    10561 gatgacacca accccaaccg ctactggagc gcgggcaacc cgcagaactc cgtcaaggtc
    10621 gcgggcacgg gcacgaagat caccgtcaag aaatcgctca gccgcggctg ggtcctggac
    10681 gtggcggtcc gcttctgacc caccggccgc cacccgcagt gagcgggagg tgaccacctg
    10741 aggggcccgg gatgcgcatg catcccgggc ccttcgtcat agccgacgcc tcgtcatacc
    10801 ggacaccgcg tcagcccgac accgcgtcat acccgcgccg caggcacccg gttaacaggc
    10861 gcgtaacacg cctgcggttg gatggccgta tggatcgtca gcaggagttc gtcctgcgca
    10921 ccatcgaaga gcgggacatc cggttcgtgc gcctgtggtt cacggacgtc ctcggcacgc
    10981 tgaagtcggt ggccatcgcc ccggccgagc tggagggggc gttcgcggag ggcatcgggt
    11041 tcgacggctc ggtcatcgag gggttcgccc gcgtcttcga gtcggacatg ctcgccaagc
    11101 ccgaccccgc caccttccag gtgctcccgt ggcgcggcga ctcccccggc acggcccgca
    11161 tgttctgcga tatcaccttg cccgacggca ccccgagcat cgccgacccc cgcaatgtcc
    11221 tcaagcgagc cttggccaag tgctcggaca tgggctacac gttctacacc caccccgaga
    11281 tcgagttctt cctgctcaag cggggctacg agccgggggt caagcccgag ccgatcgaca
    11341 acgccggcta tttcgaccac gtgcacaccg gtgccgcgca ggacttccgg cgcaccgcga
    11401 tctcgatgct cgagtcggtc gggatctcgg tcgagttcag ccaccatgag ggcgccccgg
    11461 ggcagaacga gatcgacctg cggtatgccg atgcgctcac caccagcgac aacatcatga
    11521 ccttccgcac catcctcaaa gaggtcgcct acgaacgcga catctacgcg accttcatgc
    11581 cgaaggtgtt cgccgatcac cccggctcgg gcatgcacac acacatgtcg ctcttcgacg
    11641 gcgacaccaa cgccttcttc gaggccggcg ccgactatca actctccgct gctgcccggc
    11701 aattcatcgc cggcatcctg catcacggcg ccgagatcac cgcggtcacc aaccagtggg
    11761 tcaactccta caagcgactg tggggcgggg gcgaggcgcc cgcgcacctg acgtgggggc
    11821 acaacaacac ctccgcgatg gtgcgcgtgc cgatgtacaa gcccgacaag ggcaacagca
    11881 cccgcatcga agtgcgcacc atcgacccgg cctgcaaccc ctacctcgcg tatgccgtgc
    11941 tgctcgccgc cggcctgaag ggcatcgagg agggctacga gctgccaccg gaggccgaag
    12001 aggacgtctg gagcctgacc cagggcgagc ggcaggccct cggcatcgca cccctgcccg
    12061 ggtcgctgca cgacgcgatc acgatcatgg aacgcagcga cctcgtcgcc gagaccctcg
    12121 gcgagcacgt cttcgacttc ttcctgcgca acaagcagca ggagtgggcc gactaccgcc
    12181 gccaggtcac cgagttcgaa ctcgcgcgat acctcgaccg gttgtagttc tgtggtccgc
    12241 cggcccgggt tcgcccgggc cgatgtctct tcctcacccc gcttcgtgtt ggtcgcggcg
    12301 gtggccgcgg ataggtttgg cgtatgcggg agcgcaccga atccgccacg gccgcgttgg
    12361 cgcggctcgg gttcgccgac cccggaaatg ccgagcggct ggtcgccgat ccggcgctga
    12421 ccgggctgat cgacccgctc gacaccgact tcgacgacgg ggtcgtcgag gcgctcggcg
    12481 acgtcgccga tccggatttg gcgctgctga ccctcgtgcg gctcatggag gccctgcgca
    12541 cgcggatcgg ctccaacccc gacgacgaga gcctgggcaa agaactcccc gccctcatcg
    12601 cgacgctgcg ccggccgggt gtcggccggg accgcctgct cgccgtgatc ggcgcctcga
    12661 tcgcgctggg cgaccacctc gtcgcccacc cggagcactg gcgcagcgtc gccgaggccc
    12721 ggccgcgcac cccggagcag cgcgtcgcgc ggatcgtggg ccgggtccgg aacccggatc
    12781 cggggctgag ccccgacgac gccctgcgcg tgtattaccg ccgggggctg ctcggcatct
    12841 cggcgctcga cctggtggcg cccgacccgc tcgtcaagct gcccgacacg gccgctgccc
    12901 tggccgacct cgccgcggcc gcactggaag gcgccctcgt catcgcgcaa tcggagttgc
    12961 ccgatgacgc cgcccagtgc cgtttcgccg tgatagccat gggcaagacc ggtgggcgag
    13021 aactgaatta catctccgac gtcgacgtca tcttcgtcgc cgaaccggcg gacggagtca
    13081 gcgagcagga cgcgatggcg gcggccgcgc gcctggcgac ccggctcatg acggcctgtt
    13141 cggccgcgac cgcccacggc accctgtggc cggtcgatgc cgccttgcgc ccggagggca
    13201 aggccggccc gctcgtgcga accgtcgcct cacacaagca gtactacgag cggtgggcca
    13261 agacgtggga attccaggcg ctgctcaagg cgcgcccggt ggcgggggac aaggaggtcg
    13321 gcgccgccta tctgcaggcc atggccccaa tggtctggca ggccgcctcc cgcgagaact
    13381 tcgtcgacga cgtccaggcg atgcgccgac gagtggaggc ccacatcccc agcgccgagg
    13441 ccgaccggca actcaaactc ggcccgggcg gcctgcgtga catcgagttc agcgtgcaac
    13501 tgctccaact cgtgcacggc cgcaccgacg agcgcatccg ttccggcacg accctcgatg
    13561 cgctggcggc gctcgcggca tacggctatg tcgggcgcga cgacgcggct caactcgccg
    13621 cggcataccg tctgctgcgc accctcgaac accgcataca gctgctgcgg ctgcgccgca
    13681 cccatctgat gccgacctca ccgcacgagt tgcgccggct cgggcgagcg ctcgggcacc
    13741 gggaggaccc ggcggcggcc gtcgtggccg aatggaaggc ggaggcgcgc gaagtgcggc
    13801 ggctgcacga gcggctcttc taccgcccgc tgctcggcgc agtggcacgc ctcggcaccg
    13861 acgacgctcg cctcaccccc gaagcggcac gagaacgctt gagcgccttg ggttttcgcg
    13921 accccaccgg ggcgctgcgg cacctcgagg ccctcaccga agggctctcc cggcgcgccg
    13981 cgatccagcg gcacctgctg ccggtgatgc tcggctggtt cgcggccgag gctgacccgg
    14041 acggcgggtt gctcgccttc cgcaaggtga gcgagtccct ggactcctcg cactggtacc
    14101 tgcggctgct gcgcgacgaa ccggccgccg ccgagcaact cgcccgcacg ctcgcgcgca
    14161 gcaagttcgc cgccgaggtg ctcgtcgccg cccccgaggc ggtgcagatc ctcggcgaac
    14221 ccggcggggt gcgcccgcac tcgcgtgggg acatcgagcg ccggatgcgc acggcggcgc
    14281 agcacaagac ctcgctggac gacgcgatcg ccgcggcccg gcaggtccgg cgcctcgagc
    14341 tcttccgcat cgccgtcgcc gaccttgtcg gtgatctccc gcagccggac gtggggcgcg
    14401 ccctgtccga cctgacggcc gcaaccatcg aggtcgcgct gcaattcgtc atccgcgagg
    14461 aggagaagcg cgccggccgg ccgctggtga ccgatctgct catcgtcggc atgggccggc
    14521 tcggcggcaa cgaactcggt tacgcctccg acgccgacgt gctcttcgtc cacgacccgc
    14581 acatcggcgc cgacgaggac gcggcgcagg cgcaggccct ggctgtcgtc aagtcgatgc
    14641 agcgggtgct ctcggtgccc gggcccgacc ccgccatcgg cctcgacgcc gacctgcggc
    14701 ccgaaggcaa gaacggcccg ctcgtgcgca gcctcgactc ctatcgcgcc tactacgacc
    14761 ggtgggcgct gatctgggag gcgcaggcct tggtgcgggc gcggccgatc gccggcaagg
    14821 cagaactcgc ggcccggttc gaggacctca tcgaccccat ccgctggcac ggcgccggcc
    14881 tgagcgcaca ggacctgcgc gagatccgcc ggctcaaggc gcgcatggag gccgagcggc
    14941 tgccgcgcgg cgcggacaag cggacccact tcaaactcgg cacgggcggg ctgtccgatg
    15001 tcgagtgggt cgtgcagacc ctgcagatgg agcacgccca caactacccg acgctgcgca
    15061 ccacctccac cctgccggcg ctcacggccg cgactcaggc ccggctcatc tccagcgccg
    15121 actccgccgc tctcgccaag gcctgggagt tcgccgccca cgcccgcaac gcgtcgatgc
    15181 tctatcgggg caaggcggtc gaatcggtgc ccaccgactc ccgggaggcc gacggcaccg
    15241 cccggatcat gggcctgccg ccgggttccg gccaggagtt cggggaggac taccggcgca
    15301 tcacccgtca cgcccgcgcc gtcgtcgacc gcgtcttcta cgggcgcgag gagagctgag
    15361 ccagctttcg gacggcggcg gaagcacggc ataccggaat ctagacttgc gccatgatgc
    15421 agcgactgga tctgcgtggc cgatccctgt ccctgcgtga gcttcgcgag agcttgccca
    15481 gggcggagtt cgacgtggac gccgcgctgc atgccgtccg accgatctgc gaggaggtgc
    15541 gcggcggcgg cgccgaggcg atcaaggagt tggggcagcg gtatgacggg gtgtgccccg
    15601 atcgcctccg ggtcccggcc ggagcgctgg ccgtggcgtt ggcgcaactc gaccccgacg
    15661 tgcgggcggc cctggaggtg gccatcgagc gcgcccggcg ggtgcacgcc gaccaggcgc
    15721 gcaccgacac gacgacgcag gtcagcgcgg gcgggacggt caccgagcgg tgggtgccgg
    15781 tcgaccgggt gggcctgtat gtgcccggcg ggcgggcggt ctatcccagc tcggtcgtga
    15841 tgaacgtcgt gcccgcccag atcgccggcg tcgcctcgtt cgccgtggcc agccccccgc
    15901 agaaggagaa caccggcgac ctggtcggtt atccgcaccc gacgatcctc gccgcgtgcg
    15961 cgctgctcgg ggtcgaggag gtctacgcga tgggcggggc gcaggccatc gccggtttcg
    16021 gctatggctt caccgacggc gacgacgtgt gcgagccggt cagcctggtc accgggccgg
    16081 gcaatatcta tgtggcggcg gcgaagcggc tgctgaaggg gctcatcggc atcgacgccg
    16141 aggccggtcc gaccgagatc gccgtgctcg ccgacgccac cgccgacccg gagtatgtcg
    16201 ccgccgatct gatcagtcag gccgagcacg acccgctcgc ggcgtcggtg ctggtcaccg
    16261 atgcgccgga tctcgccgag gcggtcgccg ccgccgtcgc ccgccgggcc gccgcgacca
    16321 agcacagcga gcgcgtcatc gaggcgctgt cggggcggca gtcggcgatc gtcctcgtcg
    16381 acgatctcga ccagggcctg gccgtcgtcg atgcgtatgc cgcggagcac ctcgaggtca
    16441 tcacggccga cgcgcgggag cgggcagccc gggtgcgcaa cgcgggggcg atcttcgtcg
    16501 gaccctatgc gccggtctcg ctcggggatt atgcggccgg gtccaatcac gtgttgccga
    16561 cgggtgggtg cgcgtgccac tccagcggtc tgtcggtgca gtccttcctg cgcgggatcc
    16621 acgtcgtgga ctacacgcgc gaggcgctgg ccgaggtcgc cgggcacgtc gtgacgctcg
    16681 cccaggccga ggaccttccg gcgcacggtg aggccgtgac ggcgcgcatc cccgacgccg
    16741 cgggagcctg aaccgatggc tgagcagagc ccgaggagcg ggctgggagc actcctgcgc
    16801 gaggacctgc gcggcgaaac cgcatacggt gcaccgcaat tgagcgtgcg cgccgccctg
    16861 aacaccaacg agaactccta ccccgtcccc gacgaggtcg tcgccgacat gctcgccgag
    16921 ctcgctgcgg aactcccggg gctgaaccgc tatcccgacc gcgagttcac agcgctccgg
    16981 gaggcgctcg cggcatacct ctcgcgcggc ggcacgcccg tggaggcggg ccaggtgtgg
    17041 gccggcaacg gctccaacga ggtcctgctg cacctgctgc aggcgttcgg cggcaccggg
    17101 cgccgggcca tcgggttcac cccgtcctat tcgatgcact cgatcatcgc caccaccacg
    17161 gcgacgcagt ggatcgcggg caaacgctcc gacgacccgg ctcggctctt cacgctcgac
    17221 ccggaatccg ttgcggcgca gatcgtctcg gccgacccga acatcgtgtt catctgctcg
    17281 cccaacaacc cgacggggac ggcggtcggc ctcgacgtcg tcgaggcggc atatgccgcg
    17341 gcgccgggcg cggtggtcgt ggtcgacgag gcgtatggcg agttcgcccg cgccgggacg
    17401 ccgtcggcgc tgaccttgct ggccgggcgc gagcggctgg tcgtgacccg cacgatgtcg
    17461 aaggcgttcg cactcgccgg cgggcggctc ggctatctgg ccgccgatcc cggcctggtc
    17521 gacctgttgc ggctggtccg gatgccctat cacctctcca cgcagtcgca ggcggtcgcc
    17581 cgggctgcgc tgcggcacgc agacctgatg ctctcgacgg tcggcgtcgt caaggagcag
    17641 cgcgaccgcc tcgtgaccga actgacccgt ctcgggtatg caccggtgcc cagcgacgcg
    17701 aacttcgtgc tcttcggtgg cctggccgac gccgccgtga cgtggcaggc gctgctcgac
    17761 cgcggcgtac tggtgcgcga cgtcggcatt ccccactatc ttcgggtgac ggccgggacg
    17821 ccggaggaga ccaccgactt cctcgacgtt ctcgccgcgc tcggtcccga gcacctgctc
    17881 gatcgaggag aggcgaatta gtatggcgcg caaggcaact cgcgctcgac ggacgagtga
    17941 gtccaatgtc cgggtggtcg tcaacctcga cggcaccggc acgtcccgga tcacgaccgg
    18001 catcggcttc tatgaccaca tgctcggcgc gctcgccaag cacgccctga tcgacctcga
    18061 ggtcgaggcg accggcgatc tgcacatcga cgctcaccac accgtcgagg acgtggcgat
    18121 cgtgttgggt gaggcgctgg gggaggcgct gggggacaag caggggatcg cgcgcttcgg
    18181 cgacgcgacc gtcccgctcg acgaggcgct tgtccaggca gtcgtcgacg tggccggacg
    18241 gccttactgc gtgcacacag gcgagccgga ggggcaggcc tatgtcgtca tcggccagca
    18301 gtatgtcggc gccctcaccc gccacttctt cgagaccgtc gccttccgtg cccagatcgc
    18361 gctgcacgtc cgcgtcctgt ccgggcgcga cccgcaccac ctggtcgagg cccagttcaa
    18421 ggcgttcgcg cgggccctgc gggccgccat cgccccggac ccccgggtga ccggcgtgcc
    18481 cagcacgaag ggcaagctct gaccctccat gcctgactcc gacgccatcg ccgcaggcgc
    18541 cctcgtgggc aacctgctgc tggtgcgcgc ccgggccggc caggtcggcg cctgggtggg
    18601 caaaggcctg gtggcagccc acgtcacccc gctcggggac tggacggccg tgacgccggc
    18661 gcgggtgcag gccaacaccg ccccgccgta tgacgacgcc cgcaccaccc tcgccgcccg
    18721 cccggtcagc accgcgctgc gcccggccat cgggttcttc gtccacggcc gccggggcat
    18781 cgtcgtcgtc cagccccaag gctggcgggc cgtcaagcgg tggctggtct ggaccccgga
    18841 ggacggtccg gtgcgggcgc cccggttgac gaacgcgcga ccggcggatc tggcgacggt
    18901 cgcggggatc ggagcgagcg gcataccggc ggtcgtcgcg gcggtgaagt ccgcccacgc
    18961 ggcacccatc ccgtggctcg ccggcctcca cgccgccctg cacctgcccg gtcagagcct
    19021 gctcaccgag cccggctcac cgcgcggcgc cctcgtcgag ccgtcaccga agcgcgtggc
    19081 ggccttcgac catctggcac gcgaagaggc ggcccaccgg gccgaggtcg tcgacgagcc
    19141 gcagaatccc ggggagcagc agtgagtcgc aacaacgtcg tcgtcctcga ctacggcagc
    19201 ggcaatgtgc gctcggccgt gcgcgcgctc gagcgggtcg gcgcgagcgt ggaactgacc
    19261 gccgacccgg ccgcggccct cgcgtgcgac gggctgttcg tgccgggggt cggcaacttc
    19321 cacgcgtgca tggccggcct gcgcggcgcc gacggccccc ggatcatcga cgtgcggctg
    19381 tccggcgggc ggccggtgct cggtgtgtgt gtcgggatgc aggtgctctt cgacggctcg
    19441 agcgaaccgt cggccgcgcc ggtcgagggc atcggcgagt ggcccgggct ggtccagcga
    19501 ctgccggcgc ccgtggtccc gcacatgggc tggaacaccg tgtcggcgcc ggccggctcc
    19561 ctgctcttcg acggcgtcgc ggatcagtcg ttctacttcg tgcactcgta tgccgcgctg
    19621 acgtgggacc tgcccgtcgc cgacggtcat ctagccgccg cgacccccct ggtgacgtgg
    19681 tgcgactacg gtgccccctt cgtcgccgcc gtcgagaacg gtcccctgtt cgccacccag
    19741 ttccaccccg agaagtccgg cgatgccggc ctgcacctgc tggagaactg ggtgcgtgcc
    19801 ctatgaccgc ccctgtgtgg aagtgagagc catgaccgac ctgccccgcc tggaactgct
    19861 gcccgccgtc gacgtgcgcg acgggcgggc cgtgcaattg cagcaggggg tcgccgggag
    19921 cgggtgggag ttcggcgacc ccgtcgaggc ggcgctcgcg tggcaggagc agggcgccga
    19981 gtggatccac ctcgtcgacc tggacgccgc gttcgggacc gggtccaatg cggacctgct
    20041 ggccgagatc gtcgggcggc tggacatcaa ggtcgagatg agcggcggca tccgcgacga
    20101 ggagacgctc gcccgggcgc tggcgaccgg gtgccggcgc gtcaacgtcg gcacggccgc
    20161 gctggagcac ccggagtgga cccggcaggc catcgcatcg tatggcgatc aggtcgccat
    20221 cgggctcgac gtgcgcgggg agacactcgc ggcccgcggc tggacgcgcg acggcggaaa
    20281 cctgtgggag accctggcac ggctcgacgc cgagggctgc tcgcggtatg tcgtcacgga
    20341 cgtcaacaag gacggcatgc tcgccgggcc caatctcgat ttgctcaaag ccgttggcca
    20401 gcacacgacg gcgccgctcg tcgcctccgg tggcgtgtcg acgctcgccg acatcgccgc
    20461 gatccgtcag ctggtcgcct ccggggtcga aggggcgatc atcgggtccg cgctctacaa
    20521 gggcgccttc accctgcccg aagcgctcga cgtcgccggc cgcccctgac tcctgcgtcg
    20581 gacgaccgcc cgtgagcaat ccacgagatc atgcccacca cccgcaccgg gtggcgccgg
    20641 gcgacgccac cgacaccgcc ggcgtccggt gggcacggcg cgacctgacg ccctccggtt
    20701 tcgagaccga catgggggac agtgacccgg cgctcgtggc cgctctcgca ggtgcggacg
    20761 aggaggcgat gatggccgcc gtcgcggccg gccgcttcct cgtggccgtc gtggcccacg
    20821 ccgaggagac tgtcacgggg gcggacgggc tcgtccgcga cgcctcggtc gacatggccc
    20881 tgatcacctt ggtcgggccg gacggtcggc gcgccctgcc ggccttcacc gggctcgccg
    20941 aactcgccgc ctgggacccg tccgcccgcc ccgtcccggt gacggccgac cgtctcggcc
    21001 aggccgccgt cgccgagagc tgcgatgtca tcgtcctcaa cctcggcgcg cccggcgagc
    21061 gggagctgcg atccagtcag gtctgggcgc tggcgatgcg gcggggctgg accacaccgc
    21121 ataccgatcc cttcgtcgcc gccgccgtcg agaccggcct cgccggttcc cccgacgtcc
    21181 aggcccatgc cctatatgcc gcgcaacccc ccggaaccct tggggtccag ttgacgttga
    21241 ggccgggtat gacgcaggcg caggtcaacg gcctgctcac gagcgtgggg gagcggctgg
    21301 ccgccgacgg cgagttccgc gcccggatcg acggtctggc cttccggctg ggctgatcat
    21361 gtggtcggag tcggccggca aggctgctag cattgacgca actgattcgt cgtgcccggg
    21421 cccgcccgag cagggcggat gctcaagaga agtcgacggc agtcaacttc cacccgcgct
    21481 ctaccgcctc aggtagccgg gtccagggtt cccggctcga tcgggaccgg cgcattccgt
    21541 gaccgacacg ggcacggatg cgacggcata cccgccgctg tttcaccggc tgccgatcga
    21601 cctctcgcga gcctcgcggg aggtcttcgt cgtttatggc gcggaccgag cgcgagcgac
    21661 acccctgaca accgagcaag gagcaccaca tcagcgagcc tcgcatcaac gaccggatcc
    21721 gcgtccccga ggtgcgtctg gtcggcccca acggcgagca ggtcgggatc gtccgcgtcg
    21781 aggacgctct gcgcctggcc gccgaggccg atcttgacct cgtcgaagtc gcaccgatgg
    21841 ccaagccccc cgtggccaag ctcatggact acggcaagta caagtacgaa gccgccatga
    21901 aggcccggga ggcccgcaag aaccaagtca ataccgtcat caaggagatc aagctccgcc
    21961 cgaagatcga cgaccacgac tacggcacca agaagggcca cgtggagcga ttcctcaagg
    22021 cgggggacaa ggtcaaggtc acgatcatgt tccgtggtcg tgagcagtcc cgtcccgagc
    22081 tcggtttccg cctcctgcag cggctggccg ccgacatcgc cgacctcggc ttcgtcgagt
    22141 cctcgcccaa gcaggacggt cgcaacatgg tcatggtgat cggcccgacc aagaagaagt
    22201 ccgaggcccg cgccgagcag cgtcgccggc aggccgagca cgacgtcccg gaggccacct
    22261 ccgaggccga ggctccggcc agcgccccag cggtcagtga caccgacgtc accgacaccc
    22321 ccgaggcgtc ctgagacctg acgggacaca cccgtcgaca cgcacacccc ggcggcagcc
    22381 cgctgccgct accagcaaag gagatcggct caccatgccg aagaacaaga cccacagtgg
    22441 ggccaagaag cgcttccgca tcacgggcag cggcaagatc atgcgccagc gcgccaacca
    22501 cgtgcacaag ttccacgaga agagcccgca gcacgcgcgg cgcctcgcga acgacgtgga
    22561 gatcgcgccc gccgacgtca agcaggtcaa gcgtctcctc ggccgctgac ggccgctcaa
    22621 cccccatcgt ctgcatcaga gaagtaacaa ggagaatcac gtggcacgcg tgaagcgggc
    22681 agtcaatgcc cacaagaagc gccgggtcgt cctcgagcgc gccagcggct accgcggtca
    22741 gcgcggccgg ctctaccgca aggccaagga gcagctcaac cactccctga cctacagcta
    22801 ccgcgaccgg cgcgccaaga agggtgactt ccgtcgcctg tggatccagc ggatcaacgc
    22861 cggcgcccgc gccaacggca tgacctacaa ccggttcatc cagggcctgc gcgccgcgga
    22921 gatcgaggtc gaccgccgca tgctcgccga actcgccgtc aacgacgaag ctgctttcgc
    22981 cgcgctcgtc gagatcgcca aggccaatgt gccggcgacg ggcgacgaga aggcttctgc
    23041 ctgagcttgt ctcgacacca ctctgagcgc cgggccggcg ccccgctgac caacccccga
    23101 tccgatcggg tgcgtgcggt cgcggcactg gcccggcgct ctgtgcgtgc gggctcgggt
    23161 cgtttcctcg tggaggggcc gcaaggcgtg cgcgagctgg tgcggtatgc cgcgccgtcg
    23221 gtcgtcgatc tctacgtcac cgccgccgcg gcgcagcggt atgccgccct ggtcgagtcc
    23281 gcttccgccg ctggggtttt cgtgcactca gtgagcgacg aggtgcttgc ggcgatggcc
    23341 gatgccgaga caccgcaggg gatgctggcg gtggcgcagc agccggagcc gacgctggcg
    23401 gatgtcctgg cggcgtcgcc gcggctgctc gtgttgctgc aggaggtgcg cgaccctggc
    23461 aacctcggca cggtgatccg ggccgccgac gcctccggcg cggacgccgt gctggtaacc
    23521 gacagctcgg tggatgtctt ctctcccaag gtcgtccgct ccaccgccgg atcgctcttc
    23581 cacctgcccg tcgtcaccgg actcgacgcc gaggcgacgc tggccgaggt ggcgagtcac
    23641 ggcatacgca ccctcgccac cgagggcgaa gcggccctga ccctgccgca cgtcgacctg
    23701 gccggccggc actgctgggt gctgggcacc gaggcgcagg gactgcccga ccacctggcc
    23761 gccgcgtgtg cggagcgggt gtcgatcccg atctacggca aggccgaatc cttgaacctg
    23821 gcgatggcgg cgaccatctg tctctacgcc tcggccgccg cccagcacgg gacccaatga
    23881 gtcaatctat tgactcattg attcgttgac tcactggcga gagcatggcg atgtgcagat
    23941 cgacatcgtc gaattcgtcg ggtatctcct catcctcggc gtcctggcgg cgctgatcct
    24001 ctccgcttcg acgtccgcgc gggccgcgcg cctggagagc ttccggcgcc gacagggcgt
    24061 ccacctcacc gaggccaccg cgcctttggt gtcgaaagcc ctctcgcgca gtcggttctg
    24121 gcgtcgggtg ggggtcctcg tgacgctgct gagcggcgtg gccgtgactc tcccgatcgg
    24181 cacttgggtc cgtggcgatc cggccacgtc ggcggactgg cttgccctgc tcgcggtcgg
    24241 tgggtgggag gcgggcaccc agccgacgag ccggggaagc caagcgtcca cggcgatgca
    24301 gctcccgcac ccgtcgcgag cgcattcgtg aatctcgagg tcgaccttgc cagccccacg
    24361 ccaccatacg aacaggtccg cagtcagctt gccggcatga tcgccgccgg gcagcttgcg
    24421 gcgggacacc gcctcccggc cattcggcag ctcgcgtccg atctgggcct ggctccgggc
    24481 acgatcggcc gggtctacaa ggagctcgag gccgaggggc tcgtggcgtc ccgggtccgc
    24541 acgggcacgg tagtgctcgc cgccgccgcg gtcccggccg cgcgaaatca gcagcaactc
    24601 gccgtggccg cccgcggcta cgcgtccacc gcccgccatc tgggtctcga cctcgacgcc
    24661 gccatctccc gcctgcgcgc cgagtggatc tgagaccgac ttcactcgcg tggtcaatct
    24721 gactattgtg caggcttggt ggaataggaa gagcgagcac atgacacacc acaacgcgga
    24781 gccgatcgga tgggaccggc atgctgccgc cgtgggtcgg gtgcgcgcac agctcgcggc
    24841 cctgccgtcc ggggcgccgg tccgcctcgc caagcggacg tccaatctgt tccggccgcg
    24901 cgaggacacg ggggctcagg ggctcgatgt ctcgtgcctc gccggggtcg tcgaggtcga
    24961 cccggcctcc cgggtggctc aggtgcaggg catgtgcacc tacgagacgc tggtcgacgc
    25021 gacgctcgcg cacgggctga tgccgtatgt cgtgccccag ctgaagacga tcacgctcgg
    25081 gggagcggtc acgggcctgg gcatcgagtc gagttcgttc cgcaacggcc tgccgcacga
    25141 gtccgtgcgc gaactcgaca tcctcaccgg cagcggcgag atcctcacgg ccacggacga
    25201 caacgcgcac cgcgacctgt tccgggcctt ccccaactcc tacggctcgt tgggctacag
    25261 cgtccgcctg atgatcgacc tggagccggt ccagccgcat gtccggctcc gccacgtccg
    25321 cttcacctcg gtgccgtcgc tcgtcgacac tctcgaaaag atattggcga caagggaatt
    25381 cgaaggtcac catgtcgact tcatcgacgg ggtggtgttc tcggccgacg agtcctacct
    25441 ggttatcgcg cacttcaccg actcaacccc gccgtacacc tccgactaca ccggccggcg
    25501 gatctactac cgttcgatcc agagttggca cgaggacttc ctgacgatcc gcgactacat
    25561 ctggcgctgg gacaccgact ggttctggtg ctcccgcgga ctgttcgtgc agaaaccgct
    25621 cgtgcgccgc gtcataccca agcgctacct gcgcagcgac gtctacggca aggtcatccg
    25681 cttcgagaac aaacacgggt ggtatgccgc gctgcagcag cgccggggcg cgttgcctca
    25741 ggagcgggtc gtccaggacg tggagatccc gatcgagcgg accgccgagt tcgtcgactg
    25801 gttcttgacc aatgtgccga tcgaaccgat ttggttgtgc cccatacgtc ttcgcgacac
    25861 cagccacgga gtgcggccgt ggccccacta tccgctcgag gccggacgcg actacgtcaa
    25921 cgtcgggttc tggtccgcgg tccccatcgt ccccagcgcc gcggacggtg acgtcaacaa
    25981 ggccatcgag cacaaggtcc acgcgctcga cggccacaag tccctttatt ccgatgccta
    26041 ctacgaccag gacgagttct ggtcgctcta tggcggcgag gagtatgcgc gcgtgaaaaa
    26101 actctatgac cccgacggcc gactgcccga cctctatgcc aaggcggtgg gccggcgatg
    26161 accatgacca tcggcgcgat cgtggacgag atcttcggcc acgacgccgc cttcgggctg
    26221 aaggcgtacg acggatccag cggcggcaac cagagtgccg ggatcctcgt ggacctcaag
    26281 aacgagcgcg gcctgcgcta tctcgtcagc gcccccggcg aactcggcct cgtgcgggcc
    26341 tacgtccagg gggacctcga catcgtcggc ctcgacgagg gcgaccccta cgacctgctc
    26401 gacagcgtca aggacaagct ggccccccgg attcccgagc cccgcaaggt caaggacatc
    26461 ctcacctcgc tcggaccccg gatgttcgtg ccgccggagc tccccgatct cgagaatccg
    26521 cccggctggc gccggctcgc cgagggcctg cggcactcca aggcgcggga cgcccaggcg
    26581 atcagccacc actacgacgt gtcgaatgcc ttctacgaga aggtacttgg gccatccatg
    26641 gcctatacgt gcgcgtgcta cccccgtgac gacgcgacgc tcgaggaagc ccaggctaac
    26701 aagttcgacc tcgtgtgccg caagctcgac ctcaaacccg gcatgcggtt gctcgacgtc
    26761 gggtgcgggt ggggcggcat ggtgcgccac gccgtcacca actacggcgt caccgccctc
    26821 ggcgtgacgc tttcgcggga gcaggcggca tacggccaag cgtggatcga acgagagggg
    26881 ctgaccggcc gcgccgagat ccggcacgac gactaccgcc atgtcaccga gtcggacttc
    26941 gacgccatct cctccatcgg cctgaccgag cacatcggca tcaagaacta ccccggctac
    27001 ttcgccttcc tgcacgaacg gctcgtcgcc ggcggtcgcc tgctcaacca ctgcatcacc
    27061 cggccgaaca atcacttcag cccgatcgca aaaggcggct tcaccaaccg ctatgtcttc
    27121 cccgatgggg aactcgcccc ggtcggcacg atcatcaacg atatcgccga ccagggcttc
    27181 gaggtccggc acgtcgagga cctgcgcgag cactacgccc gcacctgtcg cgcctgggcc
    27241 aagaatcttt cggccaattg ggacgcctgc gtcggcgacg ccgggcaggc caccgctcgc
    27301 gtgtggggcc tctatctcgc gggcagctca ctcgggttca ggcgcaacga gattcagctg
    27361 caccaagtgc tggcgcagaa ggtcgggtcc ggcggagttg cgccgtatcc gctgcgaccg
    27421 gacttcggca gctgagcctg ccggaacctc ctgtcgtcct gcggtgtaat gcccgcagag
    27481 acacaggagg acggtgagcg aggacccatc cgccgaagcg gaggccatnn nnnnnnnnnn
    27541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27601 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gggtgaccgc cgacgaggac gtccacgttg
    27661 tcggcggagc ccgtccggcg acgcccggcc gtcggcgctg gggacgggcg aagatcgctg
    27721 cgagcattgg cgctctggtg ggcgggctcc tgctcgtgcc gccggtccgg gccacggtgt
    27781 tggactggat ctcgctcggt ggcgtgcgcg tgcagacgcc cacgacgtcg gccaccgggt
    27841 cccccggcac gagcacgccg cagtccgtgt cgccccgggt gacggcaacc agcccggtcg
    27901 acgagggcga gtcgctgtcc gcgctggcgc aagcgcgcac ccgagtcggc ctcgacttcg
    27961 cagtgccggc gtctttgggt ggcccgacct ccatcatggt gctccatcag ggcaaggttg
    28021 tggaaatgac ctgggggaca ggcgattctg cgctgatcat ggacgtgctc acaggctcac
    28081 tcgactatgg cttcctcaag tccgcccgcg acgtcgagtg gaccgacgtg cgcggcaaca
    28141 gcgccctatg gctacccggc gaccacgacc tgcaatggat cgatcgatcc ggtgccaccc
    28201 agacggcgcc accgcgcacg gcccgcccga ccctcgtgtg ggtcgtcccg aaggatggcg
    28261 ccgacatcac ctatcgactc gagggcgctg cgaccaagca agccgccctc gctctcgccg
    28321 ccgaggtcgg cccatgaatg agcgggccgg aacctcgacc gcggctgcgg tgtaactcac
    28381 ccaaccggcc gcacgacgtg gccgcggacc cggcgaggag gccacgcatg atgcagacac
    28441 cgatgccgct gacaccgaac agtcgcagcc attgccccca gcgccccatc gggcgcctgt
    28501 cgatcctcgg gctgctcctg gccctcctga ccctcgtcgg catcggcccg gcccacgctg
    28561 gcggcccgac gtcggtgctg gcgaccaact acaccgacgg gcgggccggt gccgccatca
    28621 ccggcacggc gatctacgac gagctcgttc gcgagatcgg cgtgaccggg atcagcggcg
    28681 gccaggcccc cacgagccag acgacgcagc cggcagaggt cagtgacgtc ccggcaattc
    28741 gcctgacgtg gttcatccac gacaaccagc cctggcgtat cgacggcatc cacgaggccg
    28801 gacagaacat ctggatctcc acccgagagt ccttcgacgg aggcgacctc tacgaggtga
    28861 agcccatgtg gcacaaggca gcccacccca agaagctccg ctcgctcctc atcgggatgg
    28921 gggtgatggt cggtgacgcg aacgagtcga cgagcgccac gccggctgcc gccccgccgt
    28981 caccggcggc cgtcgcgcct cccgctgatt cgggctcagc ggccgcgacc gccgctgccg
    29041 attcatccgc gtccgcgact cccgcgacgc ccgagacggg cacgcggctc gcgtggttgg
    29101 cgggagcggg tgccctgacg gccctggcgc tcggattcct cgccggtcga cgcaccatac
    29161 ggcgcgatgc cggcccggat cagggcgaca gcgcggtggt ctggaacacc tgacacgccc
    29221 ttcggccacg cgcgtgcggg gtcagccgac gctgatcggc tcggcggcca tcggctccca
    29281 cagcccgcgc gcgatgaaga cctcgcgcaa caggtcgggg cgatcggtga tgatgccgtc
    29341 cacgcccacg tccagcaggc ggtgcatggt cgccgggtcg tcgatggtcc agacgatgac
    29401 ctgcactccg gccgcgtgcg cgcgggcgac gagcccgtcg gcgaagatcg ccacccggcc
    29461 gaggcggctg ggcacgtgag cgtaacgggc cggcgctccg cgccagcgga tcggcaggcg
    29521 agtgcgggag gaggagatga gcccggtcag cgaacgccag ccgagggcgg tatgcacgtg
    29581 cggcgccagg tcccgcagct ggcccagcca gccgtcccag gcccccgcca cgcacacccg
    29641 ctgcgcaatg ccgggatcgg cgagcacgcc ggccagcggc gcgatcgcgg cctggtcctt
    29701 cagatcgacg gtgaaattcg ccgcgggaaa agccgccagc gcttctcgga gggtgcagat
    29761 cggctcaccg cccacgcgca gttcctggag ctgtcggagg ctgaagcggc ccaccggccc
    29821 gctcgcctcc gtgacgcggt cgaggcgctc gtcgtggaag cacaccaatt cgccgtcccg
    29881 ggtcaggcgc acgtccgtct ccaggtagcg aaagcccagc gccgtcgcgc gggcgaaagc
    29941 cgccagggtg ttctcaggcg ccagtgcggc gccgccgcgg tgcgcgatcg ccagcggtcc
    30001 ggcagcgcgg gcataccccg tggtgggcaa catgactcca tggtcgcggg gacggcgagg
    30061 gccaatgtcg cgtggacgcg cgttcgggtg cagagttcac gtcgggacag gcaagggttc
    30121 gccggatgac gaaaacacgt caccggttcg ccaccggccg gttacagtgc cggtatgacg
    30181 tcgcctgccc tgcaacgcgc actcaccgtg cgctgctggc gatttagcga gcccgcggac
    30241 gcgcgggcgc ggcgatagag ccgcggcata cccgcctcga cgaggtggtg gcgtccgggg
    30301 gccaaaccac gttcgagccc atccggcgcg cgacctagac tttgcgcgtc cccaaaccgt
    30361 ggaagagacg atgtccggac ccaatacgaa ctacgacccg gtcgaggtcg ctgccctcgc
    30421 acccgaggcc gtcgagaccg cgcgtgacgc cgccctcgcg gcgatcgcgg cggcagcctc
    30481 cctcgaggac ctcaaggccg tgcgcagcgc ccacctcggc gacaagtcgc ctttggcgct
    30541 ggccaaccgc gagatcggcg cgctgccgcc gagcgccaag gccgctgccg ggaagctcgt
    30601 cggccaggcc aagggcgtcg tcaaccaggc cgtcgccgcc cggcagagcg aactggagac
    30661 cgagcgggac gagcgcatcc tgatcgagga agccgtcgac gtcaccttgc cggcggcccg
    30721 ccggccgctg gggcgccgcc acccgatcga gctgatgagc gagcggatcg ccgacatgtt
    30781 cgtcgggatg ggctgggaga tcgccgaagg ccccgaggtc gaggcggagt ggttcaactt
    30841 cgacgctctc aacttcgatg ccgaccaccc ggcccggcag atgcaggaca ccttctatgt
    30901 cgagccgccg gatggcgggc tggtgctgcg cacgcacacc tcgccggtgc aggcgcgctc
    30961 gctgctcgag cggggcgtgc cgatcgcgat cgcctgcatc gggcgtgtct ttcgcaccga
    31021 cgaactcgat gcgacgcata tgccggcctt ccaccaggtc gagggtttgt gcgtcgacga
    31081 gggcatcacg atggcgcacc tgaagggcac cctcgacacc ctcgcgagcc acctcttcgg
    31141 ggatgggatc accacgcggt tgcggccgtc cttcttcccc ttcaccgagc cgagcgccga
    31201 gatggatctg cgctgcttcg cctgccgcgg cgaggacccg aactgtcgcg cgtgcaaggg
    31261 caccggctgg atcgagtggg gcggttgcgg catggtgaat cgaaatgtct tgtccgcgtg
    31321 cggaatcgac ccggaccgct atagcggctt tgctttcggt atggggatcg accgggcgtt
    31381 gatgttccgc accggcgtct cggacatgcg agacatgatc gaaggagatg tgcgcttcga
    31441 cgcacagttc gggatggaga tctgatgcgc gtccccgtgg attggctgcg cgagtatgtc
    31501 gcggtgcccg ccgacgcgac cggcgtccag atcgccgcgg acctggtcaa ggtcgggctc
    31561 gaggaggagg gactgcacac ctccggggtg accggtccgc tcgtcgtggg ccgcgtgctt
    31621 accaaggagc ccgagccgca gaagaacggc aagacgatca actggtgctc cctcgacgtc
    31681 ggcgacgcca atggcaccgg cgagccccaa tgggtcgtat gcggcgcgca caacttcgag
    31741 cccggcgacc tggtcgtcgt catcctcccg ggtggctcgg tgagcactcc gcaaggtccg
    31801 atggtggtca ccgctcgcaa gacctatggc cacatctcct cggggatgat cacctccgag
    31861 cgggagctcg gcatcggtga ggaccactcg gggatcatcg tgctctcgcg cttcttcgcc
    31921 gatcggcccg aggtcctggc ggggttggcc ccggggcagg acgcgatccc actgctcggc
    31981 ctggaccgcg agaccgtgga agtcaatgtc accccggacc gcggctactg cttctccatc
    32041 cgcggcatcg cgcgagagta ttcacacgcg accggcgcgg catacaccga tcccgttctg
    32101 gcacttgccg atcgggcccc ggcggccaac gagcaggggt atgccgtgca gctggccgac
    32161 gatgcgccga tccgcgggcg cgagggctgc gatcgctatg tcgcgcgcat cgtgcgcggg
    32221 gtcgacgtgc acgcgccgtc gccggcgtgg atgaaggcgc ggctgaccga ggccggtatg
    32281 cggcccatct cgctgccggt cgatgtcacc aactacgtca tgctcggcct cggccagcca
    32341 ctgcacgctt tcgacctggc caagctcgtg ggcccgattg tcgtgcgccg cgcccgcccg
    32401 ggcgagcgga tgacgacgct ggacgacgtc gatcgcgcgc tcgacccgag cgatctgctg
    32461 atcaccgaca gcgagtcgca gcgggcgctc gcgatcgcgg gcgtcatggg tggcgcggac
    32521 accgagatct cggacacgac caccgacgtc ctcatcgagg ccgcccactt cgacccggtc
    32581 tcgatcgccc ggtcggcgcg ccggcacaag ctgccgtcgg aggcctcccg ccggttcgag
    32641 cgtggcgtgg atccgcaatt ggccgctgcc gcagcgcaac tcgccgtcga gctgctcgtc
    32701 gagcatggtg gcggggtcgt cgatgacggc gtgaccgatg tcggttcccc cgtgccggca
    32761 cccgatatcg acctcgacct gagcctgccg agccggctcg tcggcgtcga ctacccgagc
    32821 gagcgggtcg tggagatcct ccgcgagatc ggctgcggcg tggaggtatc gggggagcac
    32881 gcccgggtta caccgccgtc ctggcgaccg gacctgcgca ccgagccgga cctcgccgaa
    32941 gaagtcgccc gtatcgacgg ctacgagaag atcccgtccg tggtgcccac ggtggcggtc
    33001 ggcaacggtc tcacccacga ccagcggatg cgccgcctgg tggcgaatct gctggccgcg
    33061 cagggattct cggaagtgtg gaatgcgccc ttcgtcggcg acgaccggca cgtggcgctg
    33121 ggctacgacg ccgcggcggc tcgcgcccgg accgtccgca tcgccaatcc actctcggac
    33181 gaggcgccgc tgatgcggat ctcgatcctt tcgacgctgg tggattcctt gcgccgcaat
    33241 atttctcgcg ggttcaagga tgtggcgctc ttcgagcagg gcctggtgct tgccctcgag
    33301 ggcaagcaaa ccgcggctcc gaccgaggac gtcggcgtgc ggccgtcccc gcagaccatg
    33361 gcggcgatcc acgcagccgt gccgccacag ccgcgtcatc tcggcttcgt cctggccggg
    33421 gagatcgacc gctccggcgt ctggggcgcg ggccggccgg ccgatgtcgc cgacgccatc
    33481 gccacggcgc tgggcatcac cgaagcgctc ggcctgacgg cccgcaccgt cgccgacgac
    33541 gccatgccct tccaccccgg ccgctgcgcc cgggtggagc tggccgatgg caccgtcgtc
    33601 ggtcatgtcg gtgagctgca ccccaaggtg ctgacggcgc tcgggctgcc cgcccgggtc
    33661 gtggccggcg aactcgacct ggacgccctc tcggcagcca gcggccggcc cgcgcaggct
    33721 gtcaccttga gcaccttccc ggtggcgacc accgatgtcg cgctcgtcgt cgacgagtcg
    33781 gtcaaggcgg ccgacatcga ggcggcgctg cgcacgggag cgggggagat gctcgaatcg
    33841 gtgcgcctgt tcgacatcta ccgcggcgac cagaccggcg ccggcaagaa gtcgctcgcc
    33901 tatcgcctga gcttccgcgc accggaccgg actttgacga ccgaggaggt cagcggcata
    33961 cgggaccagg ccgtcgcagc cgccggccag gcggtcggcg ccgtccagcg ctgactcagc
    34021 cccggcaccc ccacagtcga aagcgcacga cacgccgtaa agaattttct tcacggcgtg
    34081 tcgtgcactc ttgacctgag gggttttgca tgatcttgtc tttgaacgta tagtcatgca
    34141 catgtcggta cgggtcgcgg tcgcgggtgc gagtgggtat gcgggaggcg agctcgtccg
    34201 cctgctgctc gcgcaccccg aggtggagat cggggcgctg acggcgagca gcaacgccgg
    34261 gcagaccctg ggctcgatcc acccgcacct gacgccgctg cgcgaccgcc tgctcgagga
    34321 caccacggca gaggtgctgg ccgggcacga cgtcgtcttc ctcgccctcc cgcacggcca
    34381 cagcgcggcg ctggccgcgc agctgcccga tgaggtcgtg ctcgtcgact gcggcgccga
    34441 cttccgcctc gagagcgagg gggactggac caccttctac gacacgccgt atgccgggtc
    34501 ctggccctac ggccttccgg aactcccgct cgcagaaggc aaccaacgcg atctgctggc
    34561 cggcgcgaag cggatcgccg ttcccggctg ctatccgacc ggcatcagcc tggccctcgc
    34621 acccggcctg cgcgccggcc tcctcgaccc cgcggacatc gtcatcgtcg cagcctccgg
    34681 cacctcggga gcgggcaaat cccgcaaacc acatctcctc ggctccgagg tgatgggggc
    34741 catgtcgccc tacggcgtcg ggggtcggca ccggcatacg ccggagatcg agcagaacct
    34801 ctcgcgagcc gcaggggcgc ccgcgagtgt ctcgttcacg cccaccctcg cgcccatggc
    34861 ccggggcatc ctggccacct ccaccgcgcg actgaccgcc ggcgcgagcg ccgggcaact
    34921 gcgcgaggcc tatgccgcgg catacgaacg cgagccgtgc gtcgtcctgc tccaggacgg
    34981 cgtctggccg cgcaccagcg atgtcctcgg cgccaacacg gtgcacctgc aggtcaccgt
    35041 ggatcctcgg cagggtcggc tgatcgccgt ctcggcgatc gacaatctca ccaagggcac
    35101 cgccggcgcc gccgtgcaat gcctgaacct cgccctcggc ctgcccgaac tcaccggcct
    35161 gtcgctggtc ggtgtggccc cgtgagtctc gaccaggagc acgcccgatg tccgtgacca
    35221 cccccaccgg attccgcgcc gccggcatca ctgccggact gaaggccagt ggccgtcctg
    35281 atctcgcgct ggtcgtcaac gacggcccgg accgttgcgc cgcagcggtt ttcaccacga
    35341 accgcgtcca ggccgccccg gtgatctggt cgcggcaggt gctcgccgac cagcgcgtcg
    35401 acgccgtcat cctcaactcc ggcggcgcca acgcctgcac cgggccgcag ggattccagg
    35461 acacccacaa gaccgccgag gtcgtcgccg agttgctcgg catctccgcc ggagatgtcg
    35521 cggtctgctc caccggcatg atcggcgaac tgcttcccat ggacacactg ctggcggggt
    35581 gcgcccaggt cgccggcgag ctgtccggcg acggcggccc cgcggcggcg gaggcgatca
    35641 tgaccaccga cacggtcgcc aagaccgcgg catactccgg cccgggatgg agcgtcggcg
    35701 gcatggccaa gggtgccggc atgctcgccc cagccttggc gaccatgctc gtcgtgctca
    35761 cgaccgatgc ccaggtggcc ccggcggatc tggacgccgc gctgcgcgcg gccaccgcca
    35821 agaccttcga ccggatcgac tccgacggct gtatgtcgac caatgacacc gtgatcgcgc
    35881 tggcctcggg cgcgtccgcg gtcccggtcg cgccagccga tctggaggcc gcgttgacgg
    35941 aggtgtgcac cgacctggcg ctgcagctga tccgcgacgc cgaaggtgcc gcacacgaca
    36001 tccgcatcga gatcgactcc gccgcaaccg aatccgacgc cctggaggtc ggccgagcga
    36061 tcgcccgcag caacctcttc aagtgcgcca tcttcggcaa cgaccccaac tggggccgca
    36121 tcctggccgc cgccggcacc accagcgcgg ctttcgatcc cgcgacgatc gacgtctcga
    36181 tcaacggggt gatcgtatgc cgtgacagcg gcgtgggcga cgaccgcgcc ctcgtcgacc
    36241 tcaccggccg cgatgtgcac atcgtcgtgg atctgaaggc gggggaggag tcggcgacga
    36301 tctggaccaa cgacctcacg cacgactacg tccacgagaa ctccgcctac tccacctaga
    36361 aggacaggca cgatgaccgg caccaccctg cgcgccaccc ccgacggggc ggcgatcgcc
    36421 gccgcccagg ccaaggcggc caccctcacc gaggccctgc cgtggctcga gcgcttccgc
    36481 gacgccctgg tcgtcgtcaa atacggcggc aacgccatga ccgaccgggc gctgcaggag
    36541 gccttcgccg aagacgtcgc cttcctccgc tacgccggcc tgcgtgtcgt cgtcgtccac
    36601 ggcggcggcc cacagatcaa gtcgatgctc ggccggctcg gcatgagcag cgagttcaag
    36661 ggcggcctgc gcgtgacgac gccggaggtc atggatgtgg tgcggatggt gctcaccggc
    36721 caggtcggcc gcgaactcgt cggcctgctc aaccagcacg gcccgatcgc cgtcggcctc
    36781 tccggggagg acgccggcct cttcggggcg cgccggcgcg gggtcgtcgt cgacggcatc
    36841 gaggaggaca tcggcctcgt cggtgaggtc gtcaccgtgg acccgtccgc ggtgcacgac
    36901 atcatcgccg cgggccgcat cccggtcgtc tcgaccatcg cccccgacat cgaggtcgag
    36961 ggtcaagtgc tcaatgtcaa cgccgacacc gccgcggccg ctctggcggc cgcgctcggt
    37021 gcccgcaaac tcgtcgtgct caccgatgtc gagggcgtct atgccgcgtg gcccgaccgc
    37081 tcgagcctgc tctccgcgat ggacctcacc gccgccaagg acctcctcac ccgggtcgac
    37141 gaaggcatga tcccgaaact ggaggcgtgc atcaccgccg tcgaaaaggg cgtcccgcag
    37201 gcgcacgtca tcgacggccg gcagccccac tcgctgctgc tggaggtctt caccagcgaa
    37261 ggaatcggga cgatgatcac ccgagacgat gccctggacg gacctgtctg ccaaggagat
    37321 ccgctatgac cgctgagtca ccccagcccg caccacccac ccgcagcgag caactgctcg
    37381 accgatatga ggaggcgctg ctcggcgtct tcggccgccc gcagctggtg atcgaacgcg
    37441 gcgagggctg ctacatctgg gacgccgacg gcaaacgcta tctcgacctg ctggccggca
    37501 tcgccgtcaa cgccctcggc cacaaccacc cggaactcgt cgccgccgtc tccaagcagg
    37561 tcggcgaggt cgcccatatc tcgaacttct tcacctctgg tgcgcagatc gaactggccg
    37621 agcgcctgct cgccatcgcc ggcgcgccgg agggctcccg cgtcttcttc accaactccg
    37681 gcaccgaagc cctcgaaggg gcggtcaagc tcgcccgacg caccgggcgc accggcatca
    37741 tcgccagcga gggcgccttc cacggccgct cgaccggcgc tctcgccctg accgccaagc
    37801 ccgcctatcg cgaacccttc gagccgctca tccccggggt gtcgcacatc ccgtatggcg
    37861 acgccgacgc cgtggaggac gccctcgccg ccggtgacat cagcgccgtg gtgctggagc
    37921 ccatccaggg tgagggcggg gtcatcgtcc cgcccgacga ctacctgacc cgggtccgcg
    37981 agctgaccca tgccgcacgc gccctgctga tcctcgacga agtgcagacc gggatcggcc
    38041 gcaccggaga ctggttcgcc ttccagcgca gcggaatcca gcccgatgcc atcaccatcg
    38101 ccaagggtct cggcggcggc atcccggtcg gcgcgctggt caccttcggc cccgaggtct
    38161 ccggtctcct gcagccgggc atgcacggct ccaccttcgg cggcaacccg ctggcctccg
    38221 ccgccggcct ggcggtgctg agcacgatcg agcgggacgg gctgctgcgg cataccaccg
    38281 aggtcggtga cggcttcgcg gcggcggtcc tcgcgctggg ccacccgcag atccgcgagg
    38341 tccgcggccg cgggctgctg cgcggtatcg ccctgagctc accgatcgcg cccgcggtca
    38401 tggggatcgc ccgcgccgac ggcttcatcg tcaacgccgt cgccccggac gcgatccgca
    38461 tcgcgccacc gctgatcatc accgccgagc agctcgcgag cttcaccgcc gcactgcccg
    38521 gcatcctcga ccgcgccgtg aacagctgac ccgaaagccg aaaacgcatg tccgacaacg
    38581 tatccattcc agccgagacc gtccggcact tcctcgccga cgatgacctg agcccgcagg
    38641 aacaggccga ggtcctcgcc cgcgcggccg cgctcaaggc ggccccgttc agcgaccgca
    38701 gcctcgaagg cccccaagtg gtctcgatcc tcttcgacaa gccgacgctg cgcacccagg
    38761 tctccttcgt cggtgccgtc gcgcacctgg gcggccaccc gatgaccgtc gacgccaacc
    38821 tcgcgaaggt gggggagcgg gagtcggtcg ccgacgtggc gcgggttctc ggacggcaat
    38881 cggcggtcat cgtctggcgc acctacggcc aggaccgcat cgaggagatg gcgcgctatg
    38941 cgggcgtccc ggtcgtcaac gccctgaccg acgactacca cccgtgccag atcctcgccg
    39001 acctgctgac catgaccgag cacaagggca cgctcgccgg actgaccgtc ggctatgtcg
    39061 gcgacggcgg aaacaatatg gcgcactcct atctcctcgg ttgtgccctg gccggcatgc
    39121 acatccgcat cggaaccccg gccgcgcacc agcccaaggc cgagatcgtc gagcgcgccg
    39181 tcgccatcgc cgcccagacc ggcgggtcgg tcctcgtcac cgacgacccg atcgccgccg
    39241 tccacggcgc cgacgtggtc atcaccgaca cgtgggtgtc gatgggcatg gaaggcgaga
    39301 aggcggagcg gcagggcgag cactcaccct tcgaaaagta ttccgtgaca ccggaactca
    39361 tgggcctggc cgaccccgcc gccatcttcc tgcactgcct gccggcctat cgcggatatg
    39421 aggtggacgc cgccgtcatc gacggcccgc agtcggtggt ctgggacgag gccgagaacc
    39481 ggctgcacgc ccagaaggcc gtcatctcct tcctcctgga gaagtcgggc cacgccgcgg
    39541 gggcggtatg acggtcgcct cctcccgcgg tgcccgccag cagcgcatcg tcgacctgct
    39601 cggcgaacac gccgtccgct cgcagaccca gctgctggaa ctgcttgccg cagaagggat
    39661 cgaggtcacc caggcgaccc tgtcgcggga cctggtcgag ctcggcgccg agaaggtgcg
    39721 cgtcgggaag tccttgatct atgccgtgcc gggggagggc ggcgaccgga ccgtgcgccc
    39781 ggcgccggag gccgcggagg tcagtcgccg gctggcctcc cggtgccagg agttgctcgt
    39841 ctcggcggcc tgctcggcta acctcgtcgt cctgcgcacc ccgcccggcg ccgccagctt
    39901 cctcgcctcg gccatcgacc acggtcaggt cgacggcgtg ctcgggtcca tcgccgggga
    39961 tgacacgatc atgctcatca ccgaaggcgc ggcggcgagc gagtctgtcg ctgcccactt
    40021 gctcagcttg tccgggaagg aatccgcgtg accgagatag tctccctgtg gggtggccgc
    40081 ttcagcggtg gcccggccga tgcccttgcg gcactgagca agtcgaccca cttcgactgg
    40141 cggctcgcgc ggcaggatat cgccggcagc cgcgcccacg cccaggtgct cgctgccgcg
    40201 ggcctgctcg acgagccgac cctgcacgcc atgctcaccg gcctcgatga gttggagtcc
    40261 gacgtcctcg ccggcacgtt cacccccgcc gaagacgacg aggacgtgca caccgccctc
    40321 gaacgcggcc tcatcgagcg cgccggcgcc gacgtcgggg ggcggctgcg cgccggccgc
    40381 tcccgcaacg accagatcgc caccctcttc cggatgtact tacgcgagca ggcccgccac
    40441 ctcgccggcc gcgtcctgga cgtcgtcgat gcccttctcg cacaggccga acgccaccac
    40501 ggcgtcgcca tgcccggccg cacccaccta caacacgccc agccggtgct gctcagccac
    40561 cacctgctgg cgcatgtctg ggcactgctg cgtgatgtcg accgcttcgg cgactgggac
    40621 cggcgggccg acgcctcccc atacggctcc ggcgccctcg ccggttcctc cctcggcctc
    40681 gacccggaga aggtcgccgc cgagctgggg ttcgcccgct cggtcgagaa ctccatcgac
    40741 ggcaccgcgg cgcgcgattt cgccgccgag ttcgccttcg tctgcgcgat gacggcggtc
    40801 gacatctcgc gcatcgccga ggaggtcgtg ctctgggcga caaaggaatt cggcttcatc
    40861 cgcctcgaag acgcctattc gaccgggtcg agcatcatgc cgcagaagaa gaaccccgat
    40921 gtcgccgagc tggcccgcgg caaggcgggc cggctggtcg gcgacctcgc gggcctgttg
    40981 acgaccttga aggcgctgcc cctggcttac aaccgcgacc tgcaagagga caaggagccc
    41041 gtcttcgacg ccatcgacac gctcgatgtg ttgctgccgg cgttcgccgg catggtcgcg
    41101 acgatgcagt tcgacacgga acgcctggaa agccttgccc cgcaggggtt ctcgctggcc
    41161 actgatatcg ccgaatggct cgtgcggcaa ggcgtcccct tccggatcgc gcacgaggtc
    41221 gcgggcggct gtgtgcgcac ctgcgaggag cgcggtatcg agctgtggga tctcagcgat
    41281 gacgacctgg ccgcgatctc gccgcatctg acttccgggg tccgtgaggt gctctcggtg
    41341 cagggctcgc tcgcctcccg cgacgcgaaa gggggcaccg ccccggtgcg cgttgccgag
    41401 caactcaccg cggcccgcgg ggccgcgctg cgcgcccgcg agttcgcgac cggctgatcc
    41461 ctcgtgaccg ccgctcgcct gccgcgctcc ttcttcgccc gcgacgttct cgacgtcgcg
    41521 ccggcgcttc tcggcgcgca cctggtgcac ggcggagtca cgctgcggtt gaccgaggtg
    41581 gaggcgtatg ccggggagaa gggcgacccc ggatcgcacg ccttccgcgg ccaaaccccg
    41641 cgcaccgcag tgatgttcgg cccgcccggg catctctacg tctacttcac ctacggcatg
    41701 cactactgcg ccaatgtcgt caccggtgcg gacggcctcg cctccgcggt gctcctgcgc
    41761 gccggagagg tgatcgccgg gcacgaactc gccgccgccc gccgccccgg gatccccgag
    41821 cgcgactggg cccgcggccc cgcccgcctg accgtcacca tgggcctgga ccgagcgcac
    41881 aatggcgacg acctctgcgg ccgcgccgcc gagccccgga tcgaacctgc ctctgccgca
    41941 atcgatcccg cccgcatccg caccggacca cgcgtcggag tgagcggccc cgggggcgac
    42001 ggcacggcat acccctggcg gttctggctc gacggcgagc cgaccgtgag cgcgtatcgg
    42061 ccgggagtgg ttcgcacacg gcgccccaac gccaagaagg cttgaccgga gcgctcaggt
    42121 caggtcgagg aacatcacgt gcaggccgac ccggccgcga ctggccgact cgaacgccgc
    42181 cggcacggtg ccgacgatcg tgaagccgag atcctgccag aggcgcaccg cggccgtatt
    42241 ggtctcgacg acggcgttga actggatacc ggcgtagccc tcgcgccggt gccaatcgat
    42301 catctcctcg gcgaggagcc gcccgatgcc gcgccctcgc gccgcggccg cgaccatgaa
    42361 gctcgccgtg ccgatgtgcg caccgcgccc cggccggttc ggccccatct tcgcgctgcc
    42421 cagcagagtc gcgtcctcgt cctcggcgac gatggtccgg gccggcgggc gttccagcca
    42481 gagggcggcg atctgcgcgt cgctcagtcc ctcgggatag gcgtaggtct cgccggccgc
    42541 gacgatctcg cggaagagcg gcgcgaagtc cgcgacatcc gcttcggtca tctcacgcag
    42601 acgcggcata gccgtcgatc tgctctcgca catcggcttt gacctcctcg ggcgcgaacg
    42661 agacgtccac ggccgtccgt gccaatgcgg cgaggccggc cgcatcgagc ccgagaacct
    42721 ctgcggccag ggcatattcg cggttgagtg tcgtcccgaa catcggcgga tcgtcgctgt
    42781 tgatcgtcac ggtgactccc gcgtcgcgca gcgcccggac cggatggtcc tcgatgcgct
    42841 cgaccacgcg ggtggccagg ttggaggtgg gcgagatctc cagcggtatg ccgtgctgca
    42901 ccagataggc gaccagctcc gggtcgtcga cggcccggac gccgtgcccg atccgctcgg
    42961 caccgagatc gcggatcgcc gaccagatcg tctccgggcc cgtcgactcg cccgcgtgcg
    43021 gcaccgagtg caggccggcg gcctgggccc gcgcgaagtg cggagcgaac tgcgcccgcg
    43081 gcaccccgat ctccggcccg cccagaccga aggcgaccag cgcgtccggg gcgtggtcga
    43141 cggcataccc gatcgtcgca tcggcggcgg gaaccccgga ctcgccgggg atgtcgtaga
    43201 cccaccgcac caccgtgccg tggtcgcgct ccgcggccac ccgggcgtcc tctatggcct
    43261 cggtgtaggc ctcgatgggt atgccgcgca ccaccgaggt gtagggcgtc atcgtcagct
    43321 cggcataccg aacctgctgt gccgcaaggc cttcgatcac ctcgtaggtc agcagccgca
    43381 gatcctccgg cgtgcgcaac aagtccacca ccgacagata gacctcgatg aagtgcgcga
    43441 agtcggtgaa ggtgaagaag cgccgcagcc cgtcgagatc cttgggcacc gggctgtccg
    43501 gatgccgcgc cgcgagctcg gccaccgtct cgggccgtgc cgagccgaca tggtggacat
    43561 ggagttcggc cttgggtagg cccgcgatga agttcgccgt gctcgggagc atgaccgtcg
    43621 accctagccg aagcgctcga tgtcctgcag aaccgcggtg accgtccgcc gcccatgctc
    43681 ggccatccgc ggaaaagtgt tgcggtagta gtctattccg gtcaaagaag gcagcaaggc
    43741 ccaaccgcgg gcgcgccacc aggcctcggg cccgtagtcg gccagctcct cgcgatagag
    43801 cgtccgggcg tcgcccgtga aggtccacca ggccggggcg agatccgctg ccggatcccc
    43861 gcgccgagga gtgaagtcga tgaccccact cagccggccg tcggcgacga tcaggttgcc
    43921 gggctgtagg tctccgtgca gccataccgg ctcgccggca tagtccggcg tggccaccac
    43981 ctgctcccac gcccgcgcca cggcgtccag gtcgaggccg tcgtgcttgc cgcgcaagcc
    44041 gtcgatcgcc gcccgcccac gctcggcctg cactcttggg gccgtgccgc ggctgcccgg
    44101 cgggcaaagc ggcccaccct gtgccggcac cgcgtgcaac gcccgggcga acgccgcgac
    44161 ctgccgggcg agacccggat cgtcgaggcc caggcggggt ggggtctgcc cgggaatcca
    44221 tcgcacgatc gtccacggcc acggataccc atgccccggc ctaccggcga agaccggcac
    44281 ccccaacgcc accggcacat gcggcgccag caccggcagc caagccgcct cctgcgcgcc
    44341 ctgctcgacc gcccagtcga tcttcggcat ccgcgccagc aggtccgggc cgacgcgaaa
    44401 gagcacgtga tcggtgccct ccacctccgc cggcagcggc gtcaccgcca actccgccca
    44461 ctgcggcgcc tgctgcccga tcaacgccgc caccagcgcc gcgtccgcgc gcatctcgtt
    44521 ctcgtgcacc cgcgcactcc acccgtccgc ggccgagcgg gtcaagtcct tttcgcgcgc
    44581 gcgtaggctc tgaggccatg gcgtacgacg aggtgctggc cgagcggatc cgcggggcga
    44641 tcagcggggt cgagggcgtg caggagatgg cgatgttcgg cggcctcggt tttctcgtcg
    44701 gcggccacat ggccgtcgct gccgctggca agggctggct catggcgcgt gtggatcccg
    44761 ccgagacgga ctccctgatc acccgaccgg gcgtggaacc tttcgagatg cggggtcgcg
    44821 gcatgaccgg ctggttgctc gtggccgaag atgccctcga tgacgaggca gacctgcgcg
    44881 aatgggtcga ccggggcgtt gcctacgtcg ccaccctccc cccgaagccc cggaaagcga
    44941 agaagggtta agccagtcgc aggcgcatct cgtcggtggg ggagaacccg ctcgattcat
    45001 agagggaacg cccggcatcg gaggcatgca agctcacgca gtccacgcct tgtgcacggc
    45061 attcgtcgag ggcggccctg agcaacgcgc gggccacgcc ctgcgagcgg gcgacgggct
    45121 cgacgaacat attgagcagg tagccgcgcc ggtccagctg ggtgttcatg ttcggcggaa
    45181 ggtcgagcca ggtgatgccg atcccgccga tgaccgcacc acccgattca gcgagcaggc
    45241 cgacataccg cccgtccgcc agcgcggcgg ccagccaggt gcgggcgggc ccggaggcag
    45301 ccgcgacgac ccctgcgtcg atccccatct ccgtgaacat cgcgtcccgg tgcctggtga
    45361 cggtgtccgt gtcggcggtc tgggcacggc ggatcgtcgg tcttgttgcc acgatgcgca
    45421 gtctcacatg ggagggaacg tccgcgtgac cagccgactg ccgctcgcct cctttgccta
    45481 ccggcggggg tcgtgcgtcg gcggcacacg gactaacgag cgggcgagag gaggccacgc
    45541 acgtcgacga cgtcgacggt gcgtccggtc tcggcggcct cctggaccgc cagggccacg
    45601 gccaccgctt gggtgccggc catgccggtg accgcgacgg cgcccgtgcc ctcgaccgcg
    45661 gccgtgaagt cgcgcacgct gacgacgtag aggtcctcgc ggttgcccac gtcgacagcc
    45721 cggacacccg cctcgtcgcg cagggtcagg tcgccgacgg ggtcctgggt cagcgcatcg
    45781 gtcgcgtgga gcgttccctc gctgccgtag acgtcgaccg acgaggagtt gaaggggacg
    45841 gtgaaggcgt catgagtctg tgccaggact tcatccgtcc aacgcaaggt cgtcatcacc
    45901 gcgtcgggcg cccccttggg ccatggtccc tggcgcgcca cctgggctgc ggcggtggcc
    45961 ggaggagcgt cccgcagcag gccgagcacc gcggcgtcgt ggacggtgat atccaggatc
    46021 acgccggcgc cgggcacgtc ttcgatgcgc cagccgcgca ggcgctccgg caggaagacc
    46081 gcgtggttga tacggatgcc ggtgaccgtg ccgatcgtgc cctcgtggac aagtgcggcc
    46141 agcttgcgga tgacggccga gcacgacagg tggtggttca ccccgaacac gaggccgcgg
    46201 gagtcggcga gcgcgatgag gtcgacggcc tggccgaggt cgagggtgat cggcttctcg
    46261 cacaacacat gcacgccggc ctcgagggcg gcttgcgcct gttcggcatg cttggggttg
    46321 tgcgagctga tgtatgcggc gtcgaccccc tcgagggcgg cgcccaggtc agtggtggcg
    46381 cgctcgatgc cgtgcgatgt ggcgaacgct tcagcgtgac ccggcgaccc gctcagcaca
    46441 gtgacggcgc gtccgccggt ggactcgatc gccggaatca tccgcgtggc ggcgatatcg
    46501 ctcgcgccca acaacgccca gcgcaatgac ctcatgtcga caactccctt ctgcggatga
    46561 ggctatcgcc acgaccggtg ccggccaggt ctgcctgggc acgctcatcg aggtcgagca
    46621 gcgcggcagc gatcgcatga ccccggttcc tcgcccagcc ggaacgtccc ctgatgggcg
    46681 cctcggcttc ccgcggcaac gcgtgaccgg atgagcggcc atcccacatg ggagaaaccg
    46741 gtctcggcaa ccaagttgcg catggcaggc taggcgagcg cgcggcatac cgctgccgca
    46801 ccgaccgaag ggaaccagac catcgtgagc accatcctcg acgagttgca gtggcgcgga
    46861 gcggtggccc agtccaccga cgagaccgcg ctgcgcgaag cactcgcgag cggaccgatc
    46921 acggtctatt gcggtttcga cccgaccgcg ccgtcgctgc acttcggcaa cttcgtccag
    46981 ttggtgtcgc tgcgtcgtat gcagcgggca ggccacaagg tgatctgtct cgtcggtggg
    47041 tcgacgggtc tgatcggcga tcccaagccg accagcgaac gggtgctgaa gacgaaggag
    47101 cagacggccg agaacgtcgc ccggatcaag gagttggtgc ggcccttcct cgacttcgag
    47161 ggcaccgatc cctcgtgcgc ctacccggcc atcctcgtcg acaacctcga ctggaccgct
    47221 ccgatgtcgg cgttggactt cctgcgcgat gtcggcaagc acttccgcgt caaccagatg
    47281 atcaagaagg aagcgatctc ggcgcgcctg gagagccagg agggcatctc ctacaccgag
    47341 ttcagctacc agctgctcca ggcactcgac ttcctcgagc tcttccgcgc gcatgggtgc
    47401 acgatgcaga tcggcggctc ggaccagtgg ggcaacctca ccgccggc
//
LOCUS       DAOPJG010000004        44871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_864_length_44871_cov_7.586509, whole genome shotgun sequence.
ACCESSION   DAOPJG010000004 DAOPJG010000000
VERSION     DAOPJG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 44871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44871
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_864_length_44871_cov_7.586509"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKU75_00975"
     CDS             complement(<1..489)
                     /locus_tag="PKU75_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPF79476.1"
                     /translation="MLTGVLDTLRGDASVQGALTALGEPAAPDSTGIEISLPTGVRAP
                     LLALASAGRGTLLAVTATAREAEDLAAALAAYLPEDSIATFPSWETLPHERLSPRSDT
                     VGRRLAVLRRLAHPDPGDPALGEIAVLTCSVRALLQPIVKGLGELAPVRLHAGEERPL
                     ERV"
     gene            complement(493..1086)
                     /gene="pth"
                     /locus_tag="PKU75_00980"
     CDS             complement(493..1086)
                     /gene="pth"
                     /locus_tag="PKU75_00980"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPF79477.1"
                     /translation="MTDPWLVVGLGNPGARYAANRHNVGAHVIAELAGRQRLSLSAHK
                     GGASAVRTHLPGPGGIPGVLIVPSSYMNVSGPPVRELASFYKIPPERIVVIHDELDID
                     FGLVRLKKGGGEGGHNGLRSITAALGTKGYHRVRVGIGRPPGRMDPADYVLRDFTSQE
                     KPEIAVTVAEAGDAVELLLTEGLLAAQQRFHSPMDGR"
     gene            complement(1083..2255)
                     /locus_tag="PKU75_00985"
     CDS             complement(1083..2255)
                     /locus_tag="PKU75_00985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF79478.1"
                     /translation="MRIAMVGLRGLPATFGGIEKHVEELGSRLVAAGHEVTVYCRPSY
                     AADPATIRPEYGYVPPVGRTPGRYRGMILRNLPAPDGKGLEAFVHSGLAAGASVGRGY
                     DIVHFHALGPGLFTPIPKYLTRAGVVQTIHGLDDERSKWGKAAKLLLGTGRRFSARVP
                     DEVVVVSKDLQRTYREVHGRESTYIPNGGPQVRHIPPGATLAKHGLEAGRYAMFLGRL
                     VPEKDPGLVIEAFRAVETDVRLAIVGDSANTDDFTAGLKAAAARDPRVVMTGYVYGDA
                     LAEIMTSAALFLQPSKLEGLPITLLEAAAHEMACVVSDIPPHLEVVGESGPGHRVFPV
                     TDAAAFADAIQAELADRQASTAGAREWSREVAAHYDWDQATAQLIEVYRRAARRRS"
     gene            complement(2257..3501)
                     /locus_tag="PKU75_00990"
     CDS             complement(2257..3501)
                     /locus_tag="PKU75_00990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF79479.1"
                     /translation="MRILQVNKFLYRRGGAEGYLLDLAEQQQAAGHDVEYFGMAHPDN
                     LPMRYAEHFPSHVEFEPAPSDPRGRLELVGRMLWSKQAYQGISAVIKDFRPDVVHMHN
                     IYHQLSPSMVRACAKAGVPVVMTLHDYKLACPTYQFLDQGEVCTACVGGSLTNAVRRR
                     CKDGSLAASAIAMVEVGAHRALKAYDPVARFLCPSAFLRDQMVAAGLHTGKMLHLDNF
                     TDTDVPVRSHPGEGVLFAGRLSHEKGVDTLVDAAGILAREVDGPVLEIVGDGPDRAAL
                     EAQAQRVAPDAIRFHGRVTADQVRGFLRGARVSAVPSRWYENQPLSVLEAYASGVPVV
                     ASAMGGLNDLVTPGVDGALVPAEDPQVLADALRPYLQDPDLSMRHGAAARQRAEERHN
                     PAGHAARIEQIYAEAISGRKAA"
     gene            complement(3518..6670)
                     /locus_tag="PKU75_00995"
     CDS             complement(3518..6670)
                     /locus_tag="PKU75_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPF79480.1"
                     /translation="MSAPTTPALGSEAAEKAPKAVVRQSGITSIVAMLGVLSGLLLDL
                     SAVGVMGAIGARSDAWVVALRLPLAVTAIAMVVANQVLVPTFAAWATRLRAWERTGAT
                     SAILVATGIATVVLAGLMSAFARPLISVIAPDFTSGEIDMAASMTRIIAWYIPFVLMA
                     EVLRSWLNAHLVVGFPAAMTLVLNLSAVAFIIAFAQHRPLMVPTAYVAGSLVQVVAMA
                     VMAFFKGWRPAAPMMRHPEVAATARLLVRPSGAAALNPVVRTLEAALATFLPTGSNTL
                     LHFGNRLAAAVGGTIVFRSIMVSVLPRISRAFGRGDTGQFRMMTRLGMRLMFLLAVPM
                     TMLGVVLAPALTEAVFSIVPKFNGPGQAGKLHTLGILLALYSLSFVGAGVQRSLLAPF
                     YAMRDTRTPLRNTVYATVANVALLLLGFALLKDHPNAIYIFPIAYSLAQCVNVAHAWW
                     RMRTVPGLERMPLLIPLAGSLVAGGVGALVAWLILRFAPATPELLAPMLATLAGLGVA
                     FGLGGLAFRRGSRGTGTGAAQFADRSMPSDQIPSAQIPSAQTLVAREQEELPVTTTDE
                     QVVGAPQPPTRRSVFDVQRYGTLGTVLTALLGIALTVLTVVEVYNGTSRLTVALPIAL
                     LVGIALLMVAFYDLETFVLVALTMRASLDALRTPGAAPDPHILLGLLLLGLGTVWLLS
                     RRAHLGRRFPMSALGVAYLIFLAVAFIGVITSASAGTAITEWSRIANIVVVFLVVEQF
                     AARGSALFPFVAAIALSILVPAAVALVQLSSGEGLFVAGGYSRITGTFDHSNPLAYFS
                     CVVLVLMVAVFPRVKDLTRVAVGSVAVIAVILLGLTYTRSAWLVAALAVFLLLLRRKS
                     YGALAVLAALLMLGISTPQVQGRFADLETSAQLSGKPANSLAWRWAYWGEALDVAKPS
                     PIAGVGLRGVAQTTAEGKQPHNDIVRAYVELGIPGLLSYVFVLALMVWTTARAARDAA
                     RRRLGGVERAIAEAAYITAISLVLLSLVANLMSQVVVTVYAVTVIALASGTYLRRRRL
                     DAEADAAADLRARSARVART"
     gene            complement(6681..7685)
                     /locus_tag="PKU75_01000"
     CDS             complement(6681..7685)
                     /locus_tag="PKU75_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79481.1"
                     /translation="MSAPLPEDRSTLGRAKARLGRHVQWAREGGIGRLIEEDNLDPRT
                     RAKAALARRAWVKANPVVPGGARAVFVVGVQRSGTNMLTSGLDAMPEVEVHNENDKKV
                     FERFQLKSHQVVLDTVAASPHQVVLFKPICDSHDTDKLLDLFGGAANPARALWAYRAV
                     DDRVRSAVTKFGDINQQVMARIAKEGIRDGDIRGPREVPERSEDTSWGGWQTGGMTPE
                     VLEAVRSCDPASLDANSGAALFWWARNSLYFTTGLDTRADVMPVSYDQVVADPRGTLE
                     RVCRFAGLPYRPEVSAHVDSRAAARGHKAPLDLDPRVRTLTDELGARLDAAAADFQVS
                     "
     gene            complement(7812..11204)
                     /locus_tag="PKU75_01005"
     CDS             complement(7812..11204)
                     /locus_tag="PKU75_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HPF79482.1"
                     /translation="MEVAPDGSLIVADTGNDRILRMSTSGAILGQYGSDAGSGDDPEN
                     ARDIAVDSSGYVYVGDAALSRVIKLSPDLKTKVDEWSGPTGDKIGSPIGLTYSNDKIY
                     VADAVKRKIRVFDTTGRQLQAITENNGCSLAAVRDADADKDGNIYVANYTNDNILKFS
                     ASGSCVDSFGSPGTGSGQFKNPYGVRVADDPYWGESVYVADSNNNRIQIFKTDGTYRT
                     QLGRTGTSDEPGTFTTLRRVAVAKDGDVWGADLWGWRLVRFDRSSSAFTYAQTIGGNP
                     PPATPGAVFNEPRAVAVSSTGNLNIIDTVNQRIVEMAPDGSIIGTCGQRDSRPISLNW
                     PRGVALDPVTGDRWVADTKQSRLQIFPAKCSAEKIRVGTKGSGTGEMNWPYSVAIRAA
                     DRLAFVADTQNNRITVWDVASRKQIASYTGAAMKQPRSVTVVEGTNKIVVSDWGNNRV
                     LELNYTTSSGFALSRAIAPGLNGPEGAGVDVNGNMWIADTGNNRVLVIDKAGTRTVVT
                     SAAGQSLKAPAAISVAGGQVFVSDTGRDRVLRYTLKSTPPPGSGPTVEKTLYSGAFAS
                     MYPVDIATTNTHYYVVDPGRYRVVGVNRSTKAIDYSVGGSRGSGTDELAAARAVATDS
                     SGNVYVADTPNNRIVKYDKTLKFIKQWGTKGTAAGQFTAIYGVAVGPGLDAGGNTTEI
                     VYTIDADRVQTFTRDGAVINGRFASGFNQPRQIEVNHLNGDVYVVNARDHQVVGFTKS
                     GTKKWAFGSAGTGNGQFTGDPRGVTVSRDGKVIFVTDDGNRRVQAWVLDSLGRPSYKY
                     SIGSPTSSTFTDPRGIEVTPDGKLVVCDEWDYSLKEFNYTTSGATLIRELFGSAAPKT
                     GANSPRGLALDSDGHLFASDWWNQRIVRSNRDGVIDLVWGQRGTRDDPGSLNFQWGVA
                     VQPGTNRVFVANRESHEITVFDHNGKWLTKWGQRGSDTASTDMTFPQGLAFDSDGTLL
                     VSDTGNGRIKRFTIDSNGNGTYKAVYGSQGTGVGQMQMPTGIDTAADGTIWVADTKNN
                     RIQSYKNGVWTAFTKPTGSTRPFAVPWGVTVHPTDGSIWVADTGRGRLVKMSTSGAMS
                     LEVTGTSAGTSDFLGPFQVVFDSSGDLFMSDTWSNRVLRLGF"
     gene            11556..13370
                     /locus_tag="PKU75_01010"
     CDS             11556..13370
                     /locus_tag="PKU75_01010"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HPF79483.1"
                     /translation="MPVKRLAATAIAAATTLAIGPGLLSAPAAASTVSPVFERYIGGP
                     GHAGLYAWGMATLRDGTILVGDYWNYRVLRFNADGTPVGTGVFIKNNGNGATQYYAPY
                     GLGQDPTTGEVYLADTDKYQIDVYSETGTYLRSWGAKGTGPGKFLYPSRVVVNSEGTV
                     YTADTWDHNIVISDRNGNEIKQFGGFGVKDGQIKSPHGLALDANDNLYVVDTGNWRVS
                     VFDKNGTFLRKFGTKGSGPGRFAGDMRGIAVNKTKGWVYVVDGEGNKISKFTLDGTFI
                     NKWGSEGHGTGQFADGGREATVDAKGNLWVADMPNYRMQKFGPNGGFLSAYPNPPQPP
                     PTGGFNAPRGVDVNPADGAVAVSDTYNWRIQTFTNAGAFVDTWGERGRGNYEFNYARL
                     LKYSPANQNIVIVDTDNGRVKAYAKDGTFLWSTGSAGTGLGQFKNPQGLGIGKDGTIY
                     VADSNNRRIQVVSKDGAAQRTFGTIDQLGFPRDVAVDPVDGTLWVSDMTKRRVTHWKT
                     DGTFLGALPVPIGTADTQLNAPFGIAADAERLYIADTASHKVKIFTKNGAFVTLFGSR
                     GTGKGKFIQPDGVALYNGRLFVAEEGTERVSVWNLNIK"
     gene            13490..15298
                     /locus_tag="PKU75_01015"
     CDS             13490..15298
                     /locus_tag="PKU75_01015"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HPF79484.1"
                     /translation="MTMTRAAIAAAAIAGAASGIPAAAEPAPPTTISPVFERTIGKSG
                     HAGLYAWGMATLRDGSILVGDYWNYRVLRFSKAGALLGVFTANHGHGANQFYTPYGLG
                     QDPRTGEVYIADTDKYQIDVYSETGTYLRSWGGQGTSPGQYLYPSRVVVNGAGTVFVA
                     DTWNHTVVMSTTAGKEIKRIGGVGTADGRMKSPHGLALDAQDNLYVADQGNKRIDVFD
                     KNGKFLRKFGSQGTGPGQFRGDLRGVAVDKKKGWVYVVDGEGNRVVKFTLAGKYLTSW
                     GRNGHGPGEFADGGREITVDANSNVWVADMPNYRVQKFTSAGTYLGEYPSPAAPPPEG
                     GFNAPRGVGVNPVDGAVAVSDTYNWRIQTFTNAGTFVKAWGERGRGNYEFNYARGVAY
                     SPLNEDIVVTDTDNGRIKAYTNKGVFRWSTGTAGTAVGQFRNPQGLTVAPDGTIYVAD
                     SNASRIQALGPEGQVLGAFGRPGQLGYPRGIAVDPADGTLWVADMTKGRVTHWRTDGT
                     FLGALPTTVGTADDQLLRPFGVAADKERVYVADTPAHKVKVFTKAGRLVTVIGGRGKV
                     NGKLLEPSALAMHHGRLYICEEGNERISIFNLRQSG"
     gene            complement(15397..17160)
                     /locus_tag="PKU75_01020"
     CDS             complement(15397..17160)
                     /locus_tag="PKU75_01020"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPF79485.1"
                     /translation="MSALAGLSAAALIAQTAPAGAQEPVGAPKVLGGRGHALVYPSGM
                     EVAPDGTLVVADTGNDSVAKFTAAGVLKWRVSAGVGAGDEAENARDIAVDNQGFIYVA
                     DAAEFRIIKLDGATGKTLMTFNGPSGDRLGSPIGITATNNKIYVSDGAKKKVRVFDTS
                     GNQINAFTANGACDLSAVRDADADAAGNVYVANYKFNNIVKFRADGTCDKTWGTQGTA
                     NGQFKNPYGVRLAKDPKWGEVVYVADSNNNRVQVFSKTGVWKATVGRTGAYNETGTFT
                     TLRRVAVAKDGDIWGADLWGWRLVRFNRTATGYAYAQTIGGRKPPTTSTAVFNEPRAV
                     AVGKDGNLNIMDTVNQRIVKMTKAGAIVDTCGQRDYRPVSFNWPRGVAIDPVTGDRWV
                     ADTKQSRLQIYPNKCGVQTFRLGNVGTGLKQFTWPFAITIRPSDRLAFVADSWNNRLS
                     VWDAATREPVETYAGTFKSPRAISLDAATGNLVVADSANNKIVTLSYTRGGGFTELAT
                     LAPAGLSMPEGVARDANGNYWIGDTGNDRVLIVSPSGAVLQTITAAAGKALSEPTSIT
                     VDGRTVYVSDTNNDRVLVYRI"
     gene            17412..19229
                     /locus_tag="PKU75_01025"
     CDS             17412..19229
                     /locus_tag="PKU75_01025"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HPF79486.1"
                     /translation="MPTRPITMGLIAAGTTALMVATGATASATDGSARTTISPAFERV
                     IGKKGHAGLYAWGMATGKDGNILIGDYWNYRVQKYSPSGASLGTVINNSGNGSNQYYT
                     PYGLGVDPRNGDIYMADTDRYQIDVYSAGGSYLRSWGGKGAGAGKFLYPSRVVVSSTG
                     TVYTADTWDHNVVVSTTSGAEQRVIGGFGTTNGKFRSPHGLALDAQDNLYVVDAGNWR
                     VQVFDKNGNFLRKFGTKGSGLGQFQGDMRGIAINKAQGWVYVVDGEGNKVQRFDLNGK
                     PLNKWGSQGYGPGQFADGGREATVDNAGNLWVADMPNFRVQKFSPTGKFLAAYPNTPQ
                     PPPLGGFNAPRGVAVAKDGTVGVSDTYNWRIETFTATGNPIKQWGKRGRGPYQFNYAR
                     GIADNPVNNEWIITDTDNGRIVSYTKDGTYRWASGTTGRANGQFKNPQGLTVAANGSI
                     YVADSNNARVQVLNSSGGFVRTFGTTAQFGYPRGIAVDPKDQSIWVTDMTKAAVTHWS
                     PTGAFLGKLKITKGTADNQLASPFGIAVDNERVYVADVPVHRVKVFTKTGQFVTTIAQ
                     RGYTNGKLFQPAHMDIYNDRLYIVEEGNERVSVFKLNTR"
     gene            19602..20921
                     /locus_tag="PKU75_01030"
     CDS             19602..20921
                     /locus_tag="PKU75_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPF79487.1"
                     /translation="MSRSTRALTAAVALTTAAAPFVLTTTAHAAGDLFVATTGLDSNS
                     GAATSPLRTVQAAVNKATPGTTIRIMPGSYYGNVKIKTSGTASAPIRITAQTPGTVTL
                     TYNSRPETCSNSQPAGDRTITIGGGADYWQIDNLKIHNGIYISGQKANSAYSWLTNLV
                     NKLDWSTRRKVPGHGAYNPSALGGVVPHLRKVTGVADINAAEGNKIIGNTMTGRGIYG
                     ALASNGTISGNTISKIPCGIGPGIWMMTFSNNNKVFGNDISDIAISHAAHYMQEGIRV
                     GSAADYNEIYDNYVHDLPGDGRGINTDVDGSWNHIHHNRVANVAIGYNDQMSGWGNRW
                     EYNTVTNYRVYGMGFRLMDARLAQPSKASSSNGSIVRCNNIVGPVGDRAKALGVGAMM
                     NGKFQSNNMPLIWISKNMQTYWSAQGNTWNGSSALPSKTPTLSSAGC"
     gene            complement(21005..22294)
                     /locus_tag="PKU75_01035"
     CDS             complement(21005..22294)
                     /locus_tag="PKU75_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79488.1"
                     /translation="MTTPHKTLTLALGAAVTGAAVLAAAPAGAAGSEIHVSTRGSDGN
                     AGTATSPLRTVQAAADRAKPGTTIRVHGGTYVGEIRIKNSGTASAPITVTPAGDGAVT
                     LTSNQSPDSCYSSAPSPRRTIKMLSGADYWTFKGLNIHHGAYLSGKGSGKVFSWHAAL
                     VKKKIWQPRRAVPGAGSYNPTAARNAIPYLAKALNTALDPVVGVKFIDNTITGRGIFA
                     TLTTSGVVQGNRISKIICGSGPGVWIMNHSNFWTVTGNDVTDIAISRAAHYMQEGIRF
                     GSAANYNKITNNKVHDLQGDGRAFNTDVDSSYNTFEKNFATNVAIGYNDQMAGWNNRW
                     RNNTVTNYRQYGYGYRLMDASLSLPSMSTSTNGVVSSGNVALHPARSGAKAMGAGGMM
                     KGTFSGNNFNTFWISKNLTRYWSSYGNTWNGSPAVPK"
     gene            complement(22307..23632)
                     /locus_tag="PKU75_01040"
     CDS             complement(22307..23632)
                     /locus_tag="PKU75_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HPF79489.1"
                     /translation="MKTTPSVRLMSSGLAATVVLAGAVAAGAGPATAAGTVLEVSTTG
                     NDANSGAPGKPLKTVQAAIDKAAAGTTIKIHKGTYNQQLKIRKSGTASAPITVMPYGD
                     GAVTLTANLPMPACNNSQPDGQRTILIISGSDYWTFQGLNIVGGVYSTGKDAGSAYTW
                     HSRLVDQGNWQTRRKVPGAGSYNPTAARQVVPYLRTVTGDQDLDSNDGLKFLNNTVTK
                     KGLFFRMNSYGVASGNKISDLACGTGPGIWLITFSNGWQVTKNDVSRIAASTWKHFMQ
                     EGIRMGTASNYNKVTGNNVHDLPGDGRAYNTDVDSSWNTITGNRATNVAIGYNDQMSG
                     WGNVWKSNTVTSFRTYGYGVRLMDGKLTLPSYNSSANKINMSCNVASRPIGTAKSFGV
                     GGTMYGTYNGNAWTTAKVAKSVQRYWGKYGNTFNGKPTPPPATFAPSHC"
     gene            complement(23826..25310)
                     /locus_tag="PKU75_01045"
     CDS             complement(23826..25310)
                     /locus_tag="PKU75_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79490.1"
                     /translation="MVKKALATLAAATLVLSASACDDGAGPTGRATTPATMASGTMGV
                     VPPVLMETGKVDLGIKWNWNLKPPLAYAQQVGWGETFFEVEWCALRDRPEGTRFAQVE
                     RILGQAQALGYRLMLKIRVGNCGGGNKELDPAEGTRKSPSTFPDDPAAYQQFVTTMVK
                     LYSGRGVKSWAIENEVDANNFWSGTPEEYTRLVTMASEAIKAGDPDATILDAGISSTG
                     NGIELAGELMDAGKEQEALALYQQWFGRRNEGPQARFGMVDSVDALRSLLSDGRAKRI
                     RAMVAANWAAINSGAVTSYQLHFYENPDLLPDLLDYIRRHLTVRIPIGGWEIGTAWPG
                     DNYTEQIHGAETARLLGALMREQVSPIVYLPLAYTPGGATKVEIFRGLVTPEGASTPA
                     GEVYAAYAKYAKDSTDVAGVRTSGGTGILLVAPSGSLLVLWPDGEGSLRITGAGARSA
                     ADPAGSVPAGSTPRDPVLIELAATDREGALKAGEAVVGSPVTAG"
     gene            complement(25316..26260)
                     /locus_tag="PKU75_01050"
     CDS             complement(25316..26260)
                     /locus_tag="PKU75_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPF79491.1"
                     /translation="MTNGDSVRVVFVGGTGRSGSTVVANALGGLGDAVSVGELRYLWE
                     RGITENRLCGCGAAFADCPFWQEVLRTAYGAAVPDAQQMHQQLTAYTQLRTLPRLART
                     PAERSSAPEALAAVLAPLYEAIGTVSGKSVIVDSSKLPTYAALLAGVPGLALDVVHLV
                     RDPRAAAFSWQRKKEKNDSGGLMERRGVVKSATLWTAWNAGLERAWRGSPHYVRMRYE
                     SFATHPRESLQVLADALGLTGVDRLVDDEGRLDLPANHTVAGNPARMSIGPTAVRLDN
                     EWAGAMDRKEAAVVTALTTPLLTRYGYPRSLAAGSAEG"
     gene            complement(26274..27242)
                     /locus_tag="PKU75_01055"
     CDS             complement(26274..27242)
                     /locus_tag="PKU75_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79492.1"
                     /translation="MELRELLARLGRRLWLLVIIPLAALLLVLGLNAPSGTKYTSSVD
                     LRATNPVGDPTVASINLVAGSLVNATTSDEVLSKIAKTAGTPVKRIRDGISASRIADT
                     ELVHVTFADTDQAIVEKVMQAIPSAVQEQAFAPSLRQAQVAEDEAKNRLDTAMAELAK
                     VQKSTGLTNPDREYQTATSEVTSLKVALATAKGRPDTVTTKPIEDALKAAEKRVTEVA
                     DAQAEFTAPQFAVDNARAALAQRSAAKTDIESRQAASITESIGSEVTQLPWTTEILRR
                     AISAALIGLALAVLLLAIPEVLRRSREGSDDELIARERVRRETYEN"
     gene            27453..28364
                     /locus_tag="PKU75_01060"
     CDS             27453..28364
                     /locus_tag="PKU75_01060"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPF79493.1"
                     /translation="MTSDARPQPPLFIVGAPRSGTSLVYRALALHPDASWINNYQRRL
                     PGVSALAVLNRLGARRLDQRRAVWFGASGDNAYRYNTSRSLAERFFPQPVEGEPVFEH
                     CDVPEAWDGSPVTDKQRRLPGLLARTTGRSGGRVLLSKRIGHNRRIGLLHKLIPQARF
                     LDVTRDGRAVAYSLSRVDWWPEMPVWWWDDRTPQEWAAEGKDPLELCARHWVEETRVI
                     EEGLASVPAGSVLRIRYEDLVADPSRVLGEVAAFAGLDPDNAVWQAELSKISFPDKNA
                     AWNAEIPAEQRAALAVMDDQLNTMGYR"
     gene            28364..29242
                     /locus_tag="PKU75_01065"
     CDS             28364..29242
                     /locus_tag="PKU75_01065"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPF79494.1"
                     /translation="MTSLPPGFILGTGRCGSTLVHELIARHPATGFVTNLDDLGIRQG
                     NAQQLTLWRRLPAAVSEKGRARLAPSEGYRVLAREVSPMLVDPAQDVTSADLTPWLEK
                     RLHAFVEKRMSGLESAEIFLHKFTGWPRASLIAAAFPDARFVHIVRDGRAVANSWLQM
                     DWWKGHLGPAGWHFGPLSPELDEAWDAAGRTQPALAAIAWSILVDRHEECAAELGDRW
                     LTVRYEDVLEDSRTHLTRILEHLGLTWTDAFESGISRYSLSSGRKAAYLQDLTPAQVA
                     TMESLIGDQLARYGYR"
     gene            complement(29479..30966)
                     /locus_tag="PKU75_01070"
     CDS             complement(29479..30966)
                     /locus_tag="PKU75_01070"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPF79495.1"
                     /translation="MQTTVPRPHSQSVHWHTPDWMLPNVDKEGPGQGWRTRYRNRVIV
                     SDFLAALLACFVAALWRYQGVPNLRVALLTAVVPVVWVLVCGRSGAYNVNTLGIGLDE
                     YRSIGRALVILLALLSVTLVILNWRMSRLLFFVAVPLTFLLTVLARRILRGRLARART
                     RGHALQPTIVVGRGLAVHDMIASIQNDPMSTGMKVVGACASDLPDPKRVADVEVYGGP
                     ETALLAVRDTGAEAVVVASDASFSGKALRRLGWALSEIGVELFVHPGINEVAAPRLTL
                     RPAAGMSLLHVEGPTIGGFDAFAKRVIDVVCASLIALVSMPVWLFIAVSIKLHDRGPV
                     FFIQQRVGENGRTFPMVKFRTMVVDAEQRLLSGELSAHQQVNEMLFKHKQDPRITKPG
                     RILRRLSLDELPQLINVLRGEMSLVGPRPPLPREVDAYEPDALRRLRVRPGLTGMWQI
                     SGRSDLNWEESLRLDLWYVDNWSPVLDVMILMRTVRAVVKAEGAY"
     gene            complement(31230..31952)
                     /locus_tag="PKU75_01075"
     CDS             complement(31230..31952)
                     /locus_tag="PKU75_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPF79496.1"
                     /translation="MATSPKAATRLEEKQLSTENRLDVELRTEFGKGAARRVRRAGKV
                     PAVVYGHGTDPIHITMPGHDTMMALKHANALLTLVIDGNEQLALAKDVQRDPIKPVIE
                     HVDLVVVRKGEKVTVDVPVHTVGEAAAETVVNVESPTVQIEVEATNIPENIEVSIEGL
                     EAGAQILAAGLTMPAGATLATDPESLVVNVTAMISQEALDAELAEAEAGIEQEEPAEE
                     AAEAAEEAPAEAKAGAEADAEA"
     gene            complement(32209..33186)
                     /locus_tag="PKU75_01080"
     CDS             complement(32209..33186)
                     /locus_tag="PKU75_01080"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPF79497.1"
                     /translation="MTGITKKTRKNLMIFSGRVGRDLAEEVAELAGTELVPTQIYDFA
                     NGEIFVRYEESVRGCDAFVFQSHAAPINEAIMEQLIMVDALKRASAKRISVVLPFYGY
                     ARQDKKHRGREPISARLVADLFKTAGANRLMAVDLHTDQIQGFFNGPVDHLMALPVLS
                     AYVGEKYARQDLTIVSPDAGRIKVAEHWSRRLGNAPLAFIHKTRDIHRPNVSVANRVV
                     GDVAGRVCIVVDDMIDTGGTIVKAAEACMEAGAAGVVIAATHAIFSDPAVEKLAGSSA
                     QEVIVTNTLPLTPEQHFDKLTVLSVAPLIAEAVRAVFEDGSVTSLFDEL"
     gene            complement(33183..34679)
                     /gene="glmU"
                     /locus_tag="PKU75_01085"
     CDS             complement(33183..34679)
                     /gene="glmU"
                     /locus_tag="PKU75_01085"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPF79498.1"
                     /translation="MTSEAPAAVVILAAGEGTRMKSATPKVLHRIGGRSLLGHAIAAA
                     RATGPAYLSVVVRHQRDLVAEHIAQVDPDAIIADQDEVKGTGRAAECGLEPLPADLDG
                     TILVTYGDVPLLTGDTLNALLERHTQSDSVITVLTGDAPDPTGYGRIVRDADGHVAAI
                     VEHKDCTPEQLQIREINSGIYAFDAHVLRTNLARVGTENAQGEKYLTDVLALARGDGG
                     NVSAFQISDVIQTEGVNDRVQLATLGAVLSRRQTERWMRAGVTIVDPASTWIDVDVTI
                     GTDVTIQPGTQLLGATTIGEGATIGPEVTIRDSEIGAWAHVRRAEVEFSVVGEGATVG
                     PYSYLRAGTELGPQGKIGGFVETKNAKIGPGAKVPHLTYCGDATIGEGANIGAGTIFA
                     NYDGVAKHHSFVGRHSFVGSHTVIVSPVSVADGAYVAAGSALVNDVAPGEIAVARARQ
                     RNIPGWVERARGGTKTAAAAQTALDQNPAPAGDPGGHPGPREGEERQA"
     gene            34810..35511
                     /locus_tag="PKU75_01090"
     CDS             34810..35511
                     /locus_tag="PKU75_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPF79499.1"
                     /translation="MKHLTSRWGRIGISTAFAAQLALVAALCGCGTVGSAPGTQSSGT
                     QSYSTQESSPTQETATGTSSTPPSTASSTHPATTTVAGESVTIFLPNDKLAGAGDFCS
                     LVYPLQRPVPAGADRAEVALRQLLAGPTDAERAQGYGGWFSTATANDLIGVQVRGQTA
                     YVDLRDFSATIPNASTSCGSSILLHQLDATTSAATGADEVRYAFAGDPAAFWNWLQVG
                     CPAGGDDRCDPAPLR"
     gene            complement(35513..35591)
                     /locus_tag="PKU75_01095"
     tRNA            complement(35513..35591)
                     /locus_tag="PKU75_01095"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35554..35556),aa:Gln,seq:ttg)
     gene            35694..36290
                     /locus_tag="PKU75_01100"
     CDS             35694..36290
                     /locus_tag="PKU75_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF79500.1"
                     /translation="MTGVQRREQLIAVGRKSFAEKGFEGSSVEEIASRAKVSKPVVYE
                     HFGGKEGLYAVVVDREIADLTARVTDALSSEGSARTLIERAALALIGYVEEETDGFRI
                     LVRDSPPGQQTGSLASLMSDVATQVEHLLAAQFKARGLEAKTAPMYAQMLVGMVALTG
                     GWWMDSNRKFSREDVAAHVVNLAWNGLTGLEKNPTLHA"
     gene            complement(36295..37095)
                     /locus_tag="PKU75_01105"
     CDS             complement(36295..37095)
                     /locus_tag="PKU75_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPF79501.1"
                     /translation="MTTRWDPGQYAKFAYERSRPFHELAARVGAAEPGLVVDLGCGNG
                     PLTLALAQRWPAARIVGVDSSAQMLDAARALDADGRVEWIQGDLAQWDIGSLGAAPDV
                     VITNATLQWIPRHLELIPAWTAALAAGGWFAMQVPGNHDAASHRLMREVAITQPRAGE
                     LEAALKRGGSAAPATYLQVLAREGLSVDAWETTYLHVLDPEGAQENPVLEWVKGTGLR
                     PVIDLLTEEHERAAFVDAYAARLREAYPRTPAGVILPFRRIFAVGHKR"
     gene            37186..37704
                     /locus_tag="PKU75_01110"
     CDS             37186..37704
                     /locus_tag="PKU75_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPF79502.1"
                     /translation="MNVPPEPAPATDDVDAIVAAWHRERPDLDLTPLHVLSRVTRLAR
                     RLDLARREAFSAVALEPWEFDVLSALRRAGRPYRLSPGQLVEATLVTSGTMTNRVDRL
                     QERGLVRRLPSPTDRRGVLVELTREGAQTVDGALTALLEKERGLLTGLPAADQAQLAD
                     ALRALSTAIDPR"
     gene            complement(37759..39573)
                     /locus_tag="PKU75_01115"
     CDS             complement(37759..39573)
                     /locus_tag="PKU75_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPF79503.1"
                     /translation="MANLVNIERVTVHSGIVAVLDDISLGINTGDRVGVVGANGGGKS
                     TLLGVLAKTRRIDSGRITHNGSLRIAQVAQDDTLDPAATVRQVVLGELDEHEWASQPR
                     IREVLTGLLGDDRAGALGGLDAVVGSMSGGERRRLALAAALVADADLLLLDEPTNHLD
                     VEGVAWLAAYLAGTWARPDRALVVVTHDRWFLDAVATETWEVVGGRVESYEGGYAAYV
                     LARAERDRIAQVTAERRNNLLRKELAWLRRGAPARTSKPKFRLDAASALIDDEPPPRD
                     SVELMRFATARLGKDVIDLHDASLAFGDLPILARVTWQLAPGERIGLVGVNGAGKSTV
                     LNVLAGRQPLDSGKRKVGVTVRIGVVSQEVRELDKLAGLKVIEAIEQVRASTILDGKP
                     VSASSLAKRLGFPGPRQQARVGDLSGGERRRLQILRVLLDEPNVLLLDEPTNDLDIDT
                     LTALEDLLDGWAGTLVVVSHDRYLLERITDRQLALLGDGRLRDLPGGVDQYLELRHTL
                     PARPAAAGGKPPGPASAPAPAAGPSPAQVREARKEIARIEKQLQRLSEKADRLHEEMA
                     ARASDHQAMMTLAADLRAVADQVVDLEEAWLAAADIAG"
     gene            complement(39573..40505)
                     /locus_tag="PKU75_01120"
     CDS             complement(39573..40505)
                     /locus_tag="PKU75_01120"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPF79504.1"
                     /translation="MTSAPQLHTGVCVRVPAKVNLELRVGPPRPDGFHSLATVYQAVS
                     LFDTIEVSADRDWGIEVVGRTAAGVPADESNLALRAAHALADAAGIDRPVRIRVTKEI
                     PVAGGMAGGSADAAGALLACHRLWGLDLSPAELAAIAAGLGSDIPFLLAGGTALGGGR
                     GERVVPTATRGTYHWVFALSDEGLSTPAVYAECDRLRGAAPVPQPEPSADLLAALSFG
                     NAEAVADGMGNDLQEAALSMQPALAGVLEAGLSFGALAGIVSGSGPTLAFLCRDAESA
                     IDLAVSLTASGAAADVRKAHGPAAVGPVEVATCR"
     gene            complement(40520..41374)
                     /gene="rsmA"
                     /locus_tag="PKU75_01125"
     CDS             complement(40520..41374)
                     /gene="rsmA"
                     /locus_tag="PKU75_01125"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPF79505.1"
                     /translation="MTEAVHFLGAADIRRLAAEHGVRPTKQWGQNFVVDANTVRRIVK
                     TAAVQPDDVVVEVGPGLGSLTVGLLEAADQVLAVEIDPRLAAALPATMSAAAPERAGR
                     LRVIPADALRLTDLPVAPTKLVANLPYNVSVPVVLHLLETFPTIERVLVMVQLEVAER
                     LAADPGSRTYGAPSAKAAWYGEVRLAGTVARSIFWPVPNVDSGLVAIDRRDPPRADVR
                     REDVFAVIDAAFSQRRKMLRSALAGWAGSPAATSSVLTAAGIDPTLRGEQIDIAGYAA
                     IAAARAAI"
     gene            complement(41543..42724)
                     /locus_tag="PKU75_01130"
     CDS             complement(41543..42724)
                     /locus_tag="PKU75_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HPF79506.1"
                     /translation="MAQLGVVGVLAAGVAGVAHFDKSVALTVEGSSSNVHAFASTVGD
                     VLKDEGITVGPHDLVVPGVGTSVSDGQKIVVRYGRKLTVTIDGEKKEYWTTATTVGEA
                     LSDLGLRAEDAVLSASRSAAVGRAGLAMTMVTPKEVTVKVDGKSVTKTTTETDVKGLL
                     ALLKVKVGSKDIVKPRLDTPIQDGMKLQVTRVKTKTVTKSSTIAFETIRKNDSSLYQG
                     ETKVVTAGRKGEKVNYYTVVLHDGKEVKRSYVKTEIPTKVRNQVIAVGTKEKPAATPK
                     PSSSGSSSSGSSSSGSSSGGSSSGGSTGGSTSGAGINLANAAMWDRIAMCESGGNWHI
                     NTGNGYYGGLQFATASWLAMGGDDFAPRADLASREQQITIANRYYAIAGLSPWGCAHA
                     A"
     gene            complement(43095..43943)
                     /locus_tag="PKU75_01135"
     CDS             complement(43095..43943)
                     /locus_tag="PKU75_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPF79507.1"
                     /translation="MPHDSSRHSIPARPEPLPIPLVDNHTHLDIVRDDAPSLSLDDAL
                     AAAAAVGVTKLVQIGCDVEAAEISVRMAHEHPAIVAGVALHPNEAPRLAQSGGLAAAY
                     DRIGELARDPRVRTIGETGLDYYRTGPEGREVQHEAFRWHIALAKELGKPLQIHDRDS
                     HDDILAVLDDAGAPEQVVMHCFSGDAAFAQACLERGFYLSFAGPITFKNNHALRDALA
                     VTPLERVLLETDAPYLTPHPWRGAVNSPYLLALTARSAAAVLGIAVAHLCEAVGATAE
                     EFYGPW"
     gene            complement(43952..>44871)
                     /locus_tag="PKU75_01140"
     CDS             complement(43952..>44871)
                     /locus_tag="PKU75_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPF79508.1"
                     /translation="SYYFMGKDNITFHSQIWPAELLGYMGKGERGGEPGIYGQLNLPT
                     EVVSSEYLTMESQQFSTSRGHTILVRDVLTRYQPDALRYFICAAGPETSDADFTWREF
                     VQRTNSELVAGWGNLVNRTATMIHKNFGEIPVCGPLAEEDQAVLDVVAAGFETVGGLL
                     RRSRLRQATAEAMRVVGEVNAYISKTEPFKLKGEDQRERLGTVLHVLAQCVSDCNTLL
                     APMLPHSANAVDAVLGGTGDFMPMPRVEEVADLDDGSPYPIITGDYAATPPWARRPVE
                     SGTPIGKPAPVFVKLDESVIADELARMTS"
BASE COUNT         6946 a        14987 c        16037 g         6901 t
ORIGIN      
        1 gacccgctcc agggggcgtt cctcgccggc gtggaggcgg accggggcca gctcaccgag
       61 acccttgacg atgggctgca gcagggcacg caccgaacag gtcaggacgg cgatctcgcc
      121 gagtgccggg tcgccggggt cggggtgggc gagccggcgc agcaccgcca ggcgccggcc
      181 gacggtatcg ctgcgcggcg aaaggcgttc gtggggaagg gtttcccaac tggggaaggt
      241 cgcgatcgag tcctccggca ggtatgccgc gagcgccgcc gcgaggtcct ccgcctcacg
      301 cgccgtggcc gtgacggcca gcagggtccc ccgtccggcg ctcgcgagcg cgagcagcgg
      361 cgcccgcacg ccggtcggca gggaaatctc gatgcccgtc gagtcggggg cggccggctc
      421 accgagcgcc gtcagcgcac cctgcacgct cgcatcccca cgcagggtgt cgaggactcc
      481 ggtcagcatt gatcagcgcc cgtccatcgg tgagtggaac cgctgctgcg ccgcgagcag
      541 cccctcggtc aacaacagct cgacggcgtc accggcctcg gcgaccgtca ccgcgatctc
      601 cggcttctcc tgggaggtga agtcgcgcag cacatagtcg gccgggtcca tccgccccgg
      661 cggccggccg atgccgaccc gcacccggtg atagcccttc gtccccagtg cggcggtgat
      721 cgagcgcagc ccgttgtggc cgccctcgcc gccgcccttc ttcagccgca ccagcccgaa
      781 gtcgatgtcg agttcgtcgt ggatgacgac gatccgctcc ggcgggatct tgtagaaact
      841 cgcgagctcg cgcaccggcg ggccggagac gttcatatag ctgctcggca cgatcagaac
      901 ccccggtatg ccgcccggtc cgggcaagtg cgtgcgcacc gcgctcgctc cacccttgtg
      961 agcggacagc gaaagccgtt gccgcccagc caattccgcg atgacgtgcg cgccgacgtt
     1021 gtggcggttc gcggcatacc gagctccggg attgccgagt ccgacgacca gccaggggtc
     1081 ggtcatgacc ggcggcgggc cgcgcgccgg tagacctcga tcagctgcgc ggtggcctgg
     1141 tcccagtcgt agtgcgcggc gacctcgcgc gaccactcgc gggcgccggc cgtggaggcc
     1201 tgccggtcgg cgagttcggc ctggatcgcg tcggcgaagg ccgcggcgtc ggtgaccggg
     1261 aagacccggt gccccggacc cgactcgccg acgacctcca ggtgcggcgg gatatcgctg
     1321 acgacacacg ccatctcgtg tgccgccgcc tccagcaggg tgatcggcag gccttcgagt
     1381 ttggagggtt gcaggaagag cgcggccgag gtcatgatct cggcgagggc gtcgccgtag
     1441 acatatccgg tcatcaccac gcgcgggtcg cgggcggccg cggctttcag gccggcggtg
     1501 aagtcgtcgg tgttggcgga gtcgccgacg atggccaggc gcacgtcggt ctcgacggcg
     1561 cggaaggcct cgatcaccag gcccgggtcc ttttccggca ccaggcggcc gaggaacatc
     1621 gcatagcgcc cggcctcgag accgtgcttg gcgagtgtgg cgcccggggg gatatggcgc
     1681 acctgcggac cgccgttggg gatataggtg ctttcgcggc cgtggacctc gcgataggtg
     1741 cgctgcaggt ccttcgagac gacgacgacc tcgtcgggga cgcgggcgga gaagcggcgg
     1801 ccggtgccga gcagcagctt ggcggccttg ccccacttgc tgcgctcgtc gtcgaggccg
     1861 tggatggtct gcacgacgcc ggcgcgcgtc aggtatttcg ggatcggggt gaacaggccc
     1921 ggcccgaggg cgtggaagtg cacgatgtcg tagccgcggc cgacgctggc gccggcggcc
     1981 agcccggagt gcacgaaggc ctccaggccc ttgccgtcgg gggcgggcag attgcgcagg
     2041 atcatcccgc gataacggcc cggggtgcgc ccgaccgggg gcacatagcc gtattcgggg
     2101 cggatcgtcg ccgggtccgc ggcatacgac ggccggcaat agaccgtcac ctcgtgaccg
     2161 gcggccacca gccgggagcc cagctcctcg acatgcttct cgatgccacc gaacgtggcg
     2221 ggcagccctc gcaggcccac catcgcgatc cgcacctcag gccgcctttc gcccggagat
     2281 ggcctcggca tagatctgct cgatccgggc cgcgtgaccg gcggggttgt gccgctcctc
     2341 ggcgcgctgc cgcgccgcgg cgccgtgccg catcgacagg tccggatcct gcaggtaggg
     2401 ccgcagcgcg tcggcgagca cctgcgggtc ctcggcgggc accagggcgc cgtcgacgcc
     2461 gggggtgacc aggtcgttca ggccgcccat ggcgctggcg acgaccggga ccccactggc
     2521 atacgcctcg agcaccgaca ggggctggtt ctcataccag cgcgagggca ccgcgctgac
     2581 gcgggcgccg cgcaggaagc cgcgcacctg atcggcggtc acgcggccgt ggaagcggat
     2641 ggcgtccggg gccacccgct gcgcctgcgc ctccagggcg gcgcggtcgg ggccgtcgcc
     2701 gacgatctcc agcaccggcc cgtcgacctc tcgggcgagg atcccggccg cgtcgacgag
     2761 ggtgtcgacg cccttctcgt ggctcaggcg gccggcgaag agcacgccct cgccggggtg
     2821 ggagcgcacc ggcacgtcgg tgtcggtgaa gttgtccagg tgcagcatct tgccggtatg
     2881 cagtccggcc gcgaccatct gatcgcgcag gaaggcgctg gggcacagga agcgggcgac
     2941 gggatcgtag gccttcagcg cgcggtgagc gcccacctcg accatcgcga tggcgctggc
     3001 ggcgagcgag ccgtccttgc agcggcggcg caccgcattg gtcaacgagc cgccgacgca
     3061 ggcggtgcag acctccccct gatcgaggaa ctgataggtg gggcaggcca gcttgtagtc
     3121 gtgcagggtc atcacgaccg gcacgcccgc cttggcgcag gcgcgcacca tcgacgggga
     3181 gagctggtgg tagatgttgt gcatgtgcac cacatcaggg cggaagtcct tgatgacggc
     3241 gctgatgccc tggtaggcct gcttggacca caacatccgg ccgaccagtt cgaggcggcc
     3301 acgggggtcg gacggcgcgg gctcgaactc cacgtggctc gggaagtgct cggcgtagcg
     3361 catcggcagg ttgtcggggt gcgccatgcc gaagtattcg acgtcgtggc cggccgcctg
     3421 ctgctgctcg gcgaggtcga gcagatagcc ctcggcacca ccgcggcggt agaggaactt
     3481 gttgacctgc aggatccgca tcgctgctcc gccggcctca ggtgcgagcg acgcgggccg
     3541 agcgggcgcg caggtccgcg gccgcgtcgg cttcggcgtc cagccggcgg cggcgcagat
     3601 aggtcccgga ggccaaggcg atgaccgtca ccgcatagac ggtgacgacg acctgggaca
     3661 tcaggttggc gacgagcgag agcagcacga gggagatggc tgtgatgtaa gcggcctcgg
     3721 cgatggcccg ttcgacgccg cccagccggc gccgggccgc gtcccgggct gcgcgcgcgg
     3781 tggtccacac catcagggcg agcacgaaga cataactgag caggccgggg atgccgagtt
     3841 cgacataggc gcggacgatg tcgttgtgcg gctgcttgcc ctcggccgtg gtctgcgcga
     3901 cgccgcgcag gccgacgccc gcgatcgggc tcggcttcgc gacgtcaagc gcctcacccc
     3961 agtaggccca ccgccaggcg agggagttcg ccggcttgcc ggacagctga gcggaggtct
     4021 cgaggtcggc gaagcgaccc tgaacctgcg gcgtcgagat gccgagcatc aggagcgcgg
     4081 cgaggacggc cagcgcgccg taggacttgc ggcgcagcaa cagcaggaag accgcgagcg
     4141 cagcgacgag ccaggcgctg cgggtgtagg tcaggccgag caggatcacg gcgatgacgg
     4201 cgaccgaccc gacggcgacc cgggtcagat ccttgacccg ggggaagacc gccaccatca
     4261 gaacgaggac cacgcagctg aaataggcga gcgggttgga atggtcgaag gtgccggtga
     4321 tgcgggagta gccgccggcg acgaacaggc cctcaccgga gctgagctgc accagggcga
     4381 ccgcggcggg caccaggatc gacagggcga tcgcggcgac gaacgggaag agcgccgaac
     4441 cgcgcgcggc gaactgctcg acgacgagga agaccacgac gatattggcg atgcgtgacc
     4501 attccgtgat ggcggtgccg gccgaggccg aggtgatgac cccgatgaag gccaccgcga
     4561 ggaagatcag gtaggcgacg ccgagggcgg acatcgggaa gcggcggccg aggtgggcgc
     4621 ggcgggagag cagccacacg gtgcccaggc ccaggaggag cagaccgagg aggatgtgcg
     4681 ggtccggtgc ggccccgggc gtgcgcagcg cgtccaggga ggcacgcatc gtgagcgcga
     4741 cgaggacgaa ggtctccagg tcgtagaacg cgaccatgag caacgcgatg ccgaccagga
     4801 gagcgatcgg cagggcgacg gtcaggcggg aggtgccgtt gtagacctcg acgaccgtca
     4861 ggaccgtgag ggcgatgccg agcagcgccg tcaggacggt gccgagggtg ccgtagcgct
     4921 ggacgtcgaa gacactgcgg cgcgtcggcg gttgcggcgc gccgacgacc tgctcgtcgg
     4981 tggtggtcac gggcaattcc tcctgctcgc gggcgacgag cgtctgagct gacgggatct
     5041 gggctgacgg gatctgatct gacggcatgc tgcgatccgc aaactgtgcg gcgccggtgc
     5101 cggtgccgcg gctgccccgg cggaacgcca ggccaccgag accgaaggcc acgcccagac
     5161 cggccaacgt ggccagcatc ggggcgagga gttccggtgt cgccggggcg aaccgcagga
     5221 tcagccaggc gacgagcgcg ccgactcctc cggcgaccag actaccggca agggggatca
     5281 ggagcggcat ccgctccagg ccgggcaccg tgcgcatccg ccaccacgcg tgggcgacgt
     5341 tgacgcactg agcgaggctg taggcgatcg ggaagatgta gatcgcgttg gggtggtcct
     5401 tgagcagggc gaatccaagg agcagcaggg cgacattggc gaccgtggca taaacggtgt
     5461 tgcgcagcgg cgtcctggtg tcacgcatgg cgtagaacgg cgccaacaag gaccgttgca
     5521 cgcccgcgcc gacgaagctc agggagtaga gagcgagcag gatgccgagc gtatgcagtt
     5581 tcccggcttg gcccggcccg ttgaacttcg gaacgatcga gaagaccgcc tcggtcaacg
     5641 ccggcgccag gacgacaccc agcatggtca tcggcaccgc aagcaggaac atcagccgca
     5701 tgccgaggcg cgtcatcatc cggaactgcc cggtatcgcc gcgcccgaag gcccgcgaga
     5761 tgcgcggcaa cacgcttacc atgatcgagc ggaagacgat ggtgcccccc acggccgccg
     5821 ccaaccggtt gccgaagtgc agcagggtgt tggagccggt gggcaggaag gtcgccagcg
     5881 cggcttcgag cgtgcgcacc acggggttga gtgcggcagc cccgctggga cggaccaaga
     5941 gacgagcggt ggccgcaacc tccgggtgac gcatcatcgg cgccgccgga cgccacccct
     6001 tgaaaaaagc catcaccgcc atcgcgacga cctgcacgag cgagccggcg acgtaggcgg
     6061 tcggcaccat caggggccgg tgctgggcga aagcgatgat gaatgcgacg gcggagaggt
     6121 tgagcaccag ggtcatcgcg gccggaaaac cgacgacgag gtgcgcattg agccaggacc
     6181 gcaagacctc ggccatcagg acgaacggga tgtaccacgc gatgatccgc gtcatcgacg
     6241 ccgccatgtc gatctcaccg gaggtgaagt ccggggcgat caccgatatc agcgggcggg
     6301 cgaatgccga catcaggccg gcgagcacga cggtggcgat tccggtagcc acgaggatcg
     6361 cgctggttgc acccgtgcgc tcccacgcac gcagccgcgt ggcccaggcc gcaaaggtgg
     6421 gcacgagcac ctggttggcg accaccatgg cgatcgcggt caccgccagc ggcagccgca
     6481 gggcaaccac ccaggcgtcc gacctggcgc cgatcgcccc catgaccccg accgccgaca
     6541 ggtccagcag cagaccactc agcacgccga gcatggcgac gatcgaggtg atgccgcttt
     6601 gccggacgac ggccttcggg gccttctccg ccgcttcgga gccgagggcg ggggtggtgg
     6661 gagcgctcat gagtatgccg tcagctcacc tggaagtcgg cagccgcggc gtccaggcgg
     6721 gcgcctagtt cgtcggtcag ggttctcacc ctcgggtcga ggtcgagggg tgccttgtgg
     6781 ccgcgggcgg ccgcgcggga gtcgacgtgc gcgctgacct cgggccggta gggcaggccg
     6841 gcgaagcggc atacccgctc cagggtgcct cgggggtcgg cgaccacctg gtcgtaggag
     6901 accggcatga cgtcggcgcg ggtgtccagg ccggtggtga agtagaggga gttgcgcgcc
     6961 caccagaaca gggcggcgcc ggagttggcg tccagactcg cggggtcgca ggagcggacc
     7021 gcctcgagca cctcgggcgt cataccgccc gtctgccacc caccccacga agtgtcctcg
     7081 ctgcgctccg gtacttcgcg gggaccccgg atgtctccgt cacggattcc ctctttggcg
     7141 atgcgggcca tgacctgctg gttgatgtcg ccgaacttgg tcaccgccga gcgcacccga
     7201 tcatcgaccg cgcggtaggc ccacagggcg cgggccgggt tcgcggcgcc gccgaacagg
     7261 tcgagcagct tgtcggtgtc atggctgtcg cagatcggtt tgaagagcac gacctggtgc
     7321 gggctggcgg ccacagtgtc gaggacgacc tggtgggact tgagctggaa gcgctcgaag
     7381 acctttttgt cgttctcgtt gtggacctcg acctcgggca tcgcgtccag tccggaggtg
     7441 agcatgttgg tgccggagcg ttgcacgccg acgacgaaga cggcgcgggc gccgccgggc
     7501 acgacgggat tggccttaac ccaggcgcgg cgggccaggg cagccttggc gcgcgtccgg
     7561 gggtcgaggt tgtcctcctc gatcaggcga ccgatgcccc cctccctcgc ccattgcacg
     7621 tgccggccca ggcgggcctt ggcgcgcccc agggtggagc ggtcttcggg cagtggcgct
     7681 gacatacgcg agaggtcctt ttcgaggtcg gatggtgcgg acacggctac gccggccggt
     7741 cccccacgct cgtgggtgac cggccggcga tagcgggtca actcctcatt gtgccgctag
     7801 gggctgagga atcagaaacc gagtcgcagg acgcggttgc tccaggtgtc ggacatgaac
     7861 aggtcacccg aggagtcgaa gaccacctgg aacggcccga ggaagtcgga ggtgccggcg
     7921 gacgtgccgg tgacctcgag actcatggca ccggaggtgc tcatcttcac caggcgcccg
     7981 cggccggtgt cggcgaccca gatcgagccg tcggtcgggt gcacggtcac gccccacggc
     8041 acggcgaagg gccgggtgga cccggtcggc ttggtgaagg ccgtccagac gccgttcttg
     8101 tagctctgga tgcggttgtt cttggtgtcc gcgacccaga tggtgccgtc ggcggcggtg
     8161 tcgatgccgg tcggcatctg catctggccc acgccggtgc cctgggagcc gtagacggcc
     8221 ttgtaggtgc cattgccatt gctgtcaatg gtgaagcgct tgatccgccc gttgccggtg
     8281 tcgctgacca ggagggtgcc gtcgctgtcg aaggcgaggc cctgcgggaa ggtcatgtcc
     8341 gtgctggccg tgtccgaacc gcgctgtccc cacttggtca gccacttgcc gttgtggtcg
     8401 aagaccgtga tctcgtggct ctcgcggttg gcgacgaaga cccggttggt gccgggctgg
     8461 acagcgacgc cccactggaa gttgaggctg cccgggtcgt cgcgggtgcc gcgctggccc
     8521 cagaccaggt cgatcacgcc gtcgcggttg ctgcgcacga tccgctggtt ccaccagtcg
     8581 ctcgcgaata ggtggccgtc ggagtcgagg gcgaggccgc gggggctgtt ggcgccggtc
     8641 ttgggcgccg ccgacccgaa cagctcacgg atgagcgtgg ccccactggt ggtgtagttg
     8701 aactccttga gggagtagtc ccactcgtca cacacgacga gcttgccgtc gggcgtgacc
     8761 tcgatgccgc gggggtcggt gaaggtgctc gacgtcgggg aaccgatcga gtacttgtag
     8821 gacggccggc cgaggctgtc gaggacccac gcctggacgc ggcggttgcc gtcgtcggtg
     8881 acgaagatga ccttgccgtc gcggctcacg gtgacgccgc gggggtcgcc ggtgaactgg
     8941 ccgttgccgg tgccggcgct gccgaaggcc cacttcttgg tgcccgactt ggtgaagccg
     9001 acgacctggt ggtcacgggc gttgacgacg tagacgtcgc cgttgaggtg gttgacctcg
     9061 atctggcgcg gctggttgaa gccggaggcg aacctgccgt tgatgacggc accgtcacga
     9121 gtgaaggtct gcacccggtc ggcgtcgatc gtgtagacga tctcggtggt gttgccgccg
     9181 gcgtccaggc ccgggccgac agccacgccg tagatcgccg tgaactggcc ggcagccgtg
     9241 cccttggtgc cccactgctt gatgaacttc aaggtcttgt cgtacttgac gatccggttg
     9301 ttgggcgtgt cggcgacgta gacgttgccg gaggagtcgg tggcgacggc gcgggccgcg
     9361 gccagctcgt cggtgccgga gccgcgcgag ccgcccacgc tgtagtcgat cgccttggtc
     9421 gaccggttca ccccgacgac gcggtagcgg cccgggtcga cgacgtagta gtgcgtgttc
     9481 gtcgtcgcga tgtcgacggg gtacatgctc gcaaaggcgc cgctgtagag ggtcttctcc
     9541 accgtggggc cggagccggg gggcggcgtg ctcttgagcg tgtagcgcag cacccggtcg
     9601 cggccggtgt cagagacgaa gacctggccg ccggcgacgc tgatcgccgc gggggccttc
     9661 aggctctgcc cggccgccga ggtgacgacc gtgcgagtgc cggccttgtc gatgaccagg
     9721 acgcgattgt tgccggtgtc ggcgatccac atattgccgt tgacgtccac gccggcgccc
     9781 tcgggaccgt tcaggcccgg agcgatggcg cggctgagcg cgaacccgct gctggtggtg
     9841 tagttgagct cgaggacgcg attgttgccc cagtcggaaa ccacgatctt gttggtgccc
     9901 tcgaccacgg tgaccgagcg cggctgcttc atcgcggcgc cggtgtagga cgcgatctgc
     9961 ttgcgggagg cgacatccca gacggtgatg cggttgttct gggtgtccgc gacgaaggcc
    10021 agccggtcag cggcgcggat cgcgacgctg tagggccagt tcatctcgcc ggtgccggat
    10081 cccttggttc cgacccggat cttctccgcc gagcacttgg ccgggaagat ctgcagacgc
    10141 gactgcttgg tgtcggcgac ccaccggtca ccggtcacgg ggtccagggc cacgccacgc
    10201 ggccagttca gggagatcgg acggctgtcg cgctggccgc acgtgccgat gatcgagccg
    10261 tccggggcca tctcgacgat gcgctggttg acggtgtcga tgatgttgag gttgcccgtc
    10321 gagctgacgg ccacggcgcg gggctcgttg aagaccgcgc ccggggtcgc cggcggcggg
    10381 ttgcccccga tggtctgggc gtaggtgaag gcgctggagc tgcggtcgaa gcggaccagg
    10441 cgccagcccc acaggtcggc accccacacg tcgccgtcct tggccaccgc gacgcggcgc
    10501 agggtcgtga aagtgccggg ctcgtcggag gttccggtgc ggcccaattg cgtgcggtag
    10561 gtgccgtcgg tcttgaagat ctggatgcgg ttgttgttgg agtcggcgac gtagacgctc
    10621 tcgccccagt aggggtcgtc ggcgacgcgc accccgtagg ggttcttgaa ctgaccgctg
    10681 ccggtgccgg ggctgccgaa gctgtcgacg cagctgccgc tggcactgaa cttcaggatg
    10741 ttgtcgttgg tgtagttggc gacgtagatg ttgccgtcct tgtcggcgtc ggcgtcgcgc
    10801 acggcggcca ggctgcagcc gttgttctcg gtgatcgcct gcagctgccg gccggtggtg
    10861 tcgaagacac ggatcttgcg tttgacggcg tcagcgacat agatcttgtc gttgctgtag
    10921 gtcaggccga tgggtgagcc gatcttgtca ccggtggggc cggaccactc gtcgaccttc
    10981 gtcttcaggt ccggtgagag cttgatcaca cgggagaggg ccgcgtcacc gacgtagacg
    11041 taccccgagg agtcgaccgc gatgtcccgc gcgttctccg ggtcgtctcc gctgccggcg
    11101 tcggagccgt actgcccgag gatggccccg ctggtgctca tgcgcagaat gcggtcgttg
    11161 cccgtgtcag cgacgatcag gctgccgtcg ggggcgacct ccatgccaga ggcatagatg
    11221 agggcgtgcc ccggaccgcc caacacggtc ggtgtgggaa gtaccacagc cgaggccgat
    11281 ggggcggttg cggcgatcac accccctgtg agcacagcgg ccgtagcagc ggccaacagc
    11341 cgccggcgcg cgcgcgagtt cgtcatggtg caatgccacc cttcgcagtt ggaacgtgcg
    11401 ccgtgatttt ggcacacact ggcccccggc cagcaattac gggaagtgcg cccgcgcggg
    11461 ctaccatacc ctggggaagt ccgcaaacgc acgcgaccca tcggttgctc cagacaccct
    11521 gatcacaccg ttcacagcca gccccggggg aattcatgcc cgtcaagcgc ctcgccgcca
    11581 ctgccatcgc cgccgccacc actctcgcga tcggccccgg cctgctgagt gcaccggccg
    11641 ccgcgagcac cgtctccccc gtcttcgagc gctacatcgg agggcccggt cacgccggtc
    11701 tctacgcgtg gggcatggcc acgctgcgtg acggcaccat cctcgtcggc gactactgga
    11761 actaccgcgt actccggttc aacgccgacg gcactcccgt cggcacaggg gtgttcatca
    11821 agaacaacgg caacggcgcg acgcagtact acgcgccata cggcctgggt caggacccga
    11881 ccactggcga ggtctacctc gccgacaccg acaagtacca gatcgacgtc tacagcgaga
    11941 ccggcaccta ccttcgctcc tggggcgcca agggcaccgg cccgggcaaa ttcctctacc
    12001 ccagccgcgt ggtcgtcaac tccgagggca cggtctacac ggccgacacg tgggatcaca
    12061 acatcgtgat atcggaccgc aacggcaacg agatcaagca gttcggcggc ttcggcgtca
    12121 aggacgggca gatcaagtca ccgcacgggc tggcgctcga cgccaacgac aacctgtatg
    12181 tcgtcgacac cggcaactgg cgcgtcagcg tcttcgacaa gaacggcacc ttcctccgca
    12241 aattcggcac caagggctcc ggcccgggcc ggttcgccgg ggacatgcgc ggcatcgcgg
    12301 tcaacaagac caagggctgg gtctacgtcg tcgacggcga aggcaacaag atcagcaagt
    12361 tcaccctcga cggcaccttc atcaacaagt ggggttccga gggtcacggc accgggcagt
    12421 tcgcggacgg cggccgcgag gcaaccgtcg acgccaaggg gaacctgtgg gttgccgata
    12481 tgcccaacta ccggatgcag aagttcggcc ccaacggcgg cttcctgtcc gcttatccga
    12541 acccgcccca gccgccgccc accggcggtt tcaacgcgcc ccgcggcgtg gacgtcaatc
    12601 cggcggacgg cgcggtcgcc gtctccgaca cctacaactg gcgcatccag accttcacca
    12661 acgccggcgc cttcgtcgac acctggggtg agcgcggccg cggcaactac gagttcaact
    12721 acgcacggct gctcaagtac agcccggcca accagaacat cgtcatcgtg gacaccgaca
    12781 acggccgggt caaggcctac gccaaggacg gcacgttcct gtggtccaca ggttcggccg
    12841 gcaccggatt gggccagttc aagaacccgc agggcctcgg catcggcaag gacgggacga
    12901 tctacgtcgc cgactccaac aaccgccgca tccaggtggt gtccaaggac ggcgccgcgc
    12961 agcgcacctt cggcaccatc gatcagctcg gcttcccgcg cgatgtcgcc gtcgacccgg
    13021 tcgacggcac cctctgggtc agcgacatga ccaagcgccg cgtcacccac tggaagaccg
    13081 acgggacctt cctcggtgcg ctcccggtcc cgatcggcac cgcggacacc cagctcaacg
    13141 ccccgttcgg gatcgccgcg gacgccgagc ggctctacat cgccgacacc gccagccaca
    13201 aggtgaagat cttcaccaag aacggcgcct tcgtgaccct cttcggcagc cgtggcaccg
    13261 gcaaaggcaa gttcatccag cccgacggcg tcgccctgta caacgggcgg ctgttcgtcg
    13321 ccgaggaggg caccgagcgc gtctcggtct ggaacctcaa catcaaatag ccgcaccccc
    13381 ggcgcgtccg tttatcgcag gacttccgcg gccgaccacc acggcttagc atttccttac
    13441 ctgcccgtcc gggcgaccat ttcgggggag aaggaatgca atgaaggtca tgaccatgac
    13501 gcgcgccgcg atcgccgcgg ccgcgatcgc cggggccgcg agcggcatac cagcggcggc
    13561 cgaaccggcg ccgccgacca ccatctcgcc ggtcttcgag cgcacgatcg gcaaatccgg
    13621 gcacgccggg ctctatgcgt ggggcatggc cacgctgcgg gacggatcga tcctggtcgg
    13681 cgactactgg aactaccgcg tgctgcggtt cagcaaggcc ggcgcactgc tcggggtctt
    13741 cacggccaac cacggccacg gcgccaacca gttctacacg ccatacggtc tggggcagga
    13801 cccgcgcacc ggcgaggtct acatcgccga caccgacaaa taccagatcg acgtctacag
    13861 cgagaccggc acctatctgc ggtcgtgggg cgggcagggc acgagccccg ggcagtacct
    13921 ctacccctcg cgcgttgtcg tcaacggcgc cggcaccgtc ttcgtcgccg acacctggaa
    13981 ccacacggtg gtgatgtcga cgacggcggg caaggagatc aagcgcatcg gtggggtcgg
    14041 taccgcggac gggcggatga agagcccgca cgggctggca ctggacgccc aggacaacct
    14101 gtatgtcgcc gaccagggca acaagcgcat cgacgtcttc gacaagaacg gtaagttcct
    14161 ccgcaagttc ggcagccagg gcaccgggcc cggccagttc cgcggtgacc tgcgcggcgt
    14221 ggcggtcgac aagaagaaag gctgggtcta tgtcgtcgac ggtgagggca atcgcgtcgt
    14281 caagttcacc ctggcgggca aatacctgac cagttggggt cgcaacggcc acgggccggg
    14341 cgagttcgcc gacggcgggc gcgagatcac cgtggacgcc aacagcaatg tctgggtcgc
    14401 cgacatgccg aactatcgtg tgcagaagtt cacctcggcc ggcacatacc tcggggagta
    14461 cccgagcccg gccgctcccc cgcccgaggg cggcttcaac gccccccgcg gtgtcggcgt
    14521 caacccggtc gacggggccg tcgccgtatc ggacacctac aactggcgca tccagacgtt
    14581 caccaacgcc ggcaccttcg tcaaggcgtg gggcgagcgg ggccgcggca attacgagtt
    14641 caactacgcg cgcggcgtcg cctacagccc gctgaacgag gacatcgtcg tcaccgacac
    14701 cgacaacggg cggatcaagg cctacaccaa caagggcgtc ttccgctggt ccaccgggac
    14761 ggccggcaca gcggtcggac agttccgcaa cccccaaggc ctcacggtgg caccggacgg
    14821 cacgatctac gtcgccgact ccaacgccag ccgcatccag gcccttgggc ctgagggcca
    14881 ggtgctgggc gctttcggcc ggcccggcca actcggctat ccgcgcggca tcgccgtcga
    14941 cccggctgac ggcacgctct gggtggccga catgaccaag gggcgggtca cgcactggcg
    15001 gaccgacggc accttcctcg gcgcgctacc gacgaccgtt ggtaccgccg acgaccagtt
    15061 gctgcgcccc ttcggcgtgg ccgccgacaa ggagcgggtc tacgtcgccg ataccccggc
    15121 gcacaaggtc aaggtgttca ccaaggccgg ccggctcgtc acggtcatcg gtggccgcgg
    15181 caaggtcaac ggcaagctgc ttgagccatc cgccctcgcg atgcatcacg gccgcctcta
    15241 tatctgcgaa gagggcaacg agcggatctc gatcttcaac ctgcgacaga gcggctgacg
    15301 gcagcggggc ctggtttgcc gacgggggcc ggcttatagc ccggcccccg tcggcaaacc
    15361 cagccccgct tcatggatgg gtgccgcgtg ctcggctcag atcctgtaga cgagcacccg
    15421 gtcgttgttg gtgtcggaga cgtagacggt acggccgtcg acggtgatcg aggtcggctc
    15481 gctgagcgcc ttgccggcgg ccgcggtgat ggtctgcagg acagcaccgc tcgggctgac
    15541 gatcagcacc cggtcgttgc cggtgtcgcc gatccagtag ttgccgttgg cgtcgcgggc
    15601 gacgccctcg ggcatcgaca ggccggccgg ggcgagcgtg gccagttcgg tgaagccacc
    15661 gcctcgggtg tagctcaagg tgacgatctt gttgttggcc gagtcggcga cgacgaggtt
    15721 gccggtcgcg gcgtcgaggg agatcgcgcg cggcgacttg aaggtgccgg cgtaggtctc
    15781 cactggctcc cgcgtggcgg catcccagac cgagaggcgg ttgttccagc tgtccgcgac
    15841 gaacgccaag cggtcgctgg ggcgaatggt gatggcgaac ggccaggtga actgcttcaa
    15901 gccggtgccg acgttgccga ggcggaaggt ctggacgccg cacttgttgg gatagatctg
    15961 cagtcgcgac tgcttggtgt cggcgaccca ccggtcgccg gtcaccgggt cgatcgccac
    16021 cccgcgcggc cagttgaagg acacggggcg gtagtcccgc tgaccgcagg tgtcgacgat
    16081 ggcaccggcc ttggtcatct tgacgatccg ctggttgacg gtgtccatga tgttgaggtt
    16141 gccgtccttg ccgaccgcga cggcgcgcgg ctcgttgaag acggcggtgg acgtggtcgg
    16201 cggcttccgg ccaccgatcg tctgggcgta ggcgtagccg gtggcggtgc ggttgaagcg
    16261 aaccagacgc cagccccaca ggtcggcgcc ccagatgtcg ccatccttgg cgaccgccac
    16321 gcggcgcagc gtcgtgaagg tgccggtctc gttgtaggcg cccgtcctgc cgacggtggc
    16381 cttccacacc ccggtcttgc tgaagacctg gacccggttg ttgttggagt cggcgacata
    16441 gacgacctcg ccccacttcg ggtccttggc cagccgcacg ccgtaggggt tcttgaactg
    16501 tccgttggcc gtgccctggg tgccccaggt cttgtcacag gtgccgtccg cgcggaactt
    16561 gacgatgttg ttgaacttgt agttcgcgac gtagacattg ccggcggcat cggcgtcggc
    16621 gtcccggacg gccgacaggt cgcaggcgcc gttggcggtg aaggcgttga tctggttgcc
    16681 gctcgtgtcg aagacgcgga ccttcttctt cgcgccgtcg gagacgtaga tcttgttgtt
    16741 ggtggcggtg atgcctatcg ggctgccgag ccggtcaccc gaggggccgt tgaaggtcat
    16801 cagcgtcttg ccggtggctc cgtccagctt gatgatccgg aactcggctg cgtcggcgac
    16861 atagatgaag ccctggttgt cgacggcgat gtcgcgggcg ttctcggcct cgtcgccggc
    16921 gccgaccccc gccgagacgc gccacttcag cacgccggcc gcagtgaatt tggcgacgga
    16981 atcgttgccg gtgtcggcga cgacgagagt gccgtccggg gcgacctcca tccccgacgg
    17041 atagaccagg gcatgacccc ggcccccgag aaccttgggt gcgccgacgg gctcctgggc
    17101 ccctgcgggc gcggtctggg cgatcagtgc tgcggcgctc agcccagcga gcgcagacat
    17161 ggacatagtg agtttgcgca cgagtgaagc tccccctcgg aattcttggc cggggctgtg
    17221 acgccgcagc tgatggatgc ggcctcatct tcgcacgtcg cggccccgat ccgaagcccc
    17281 ttcctggtcc aaaccgtggc gatcgtcacc cctggttggt tacgatgtgt cgaccccgcg
    17341 ccaatctgcc ttcgtccgtg cgccgggtgt gactgagagc gaacggggaa cccccaatga
    17401 cattgcgaac catgccgacg cgcccgatca ccatggggtt gatcgccgcc gggacgacag
    17461 cattgatggt tgcgaccggc gctacggcgt ccgcgaccga cggcagtgca cgcacgacca
    17521 tcagcccggc cttcgagcgc gtgatcggca agaagggcca cgcggggctc tacgcctggg
    17581 gcatggccac cggcaaggac ggcaacatcc tcatcggtga ctattggaac taccgggtgc
    17641 agaagtacag cccgagcggc gccagcctgg gcaccgtcat caacaacagc ggcaacggca
    17701 gcaaccagta ctacacgccg tacggcctgg gcgtcgatcc ccgcaatggc gacatctaca
    17761 tggcggacac cgaccggtac cagatcgacg tctactccgc cggcggcagc tacctgcgca
    17821 gctggggcgg caagggcgcg ggcgcgggca agttcctcta tcccagccgc gtcgtcgtca
    17881 gcagcaccgg cacggtctat acggccgaca cgtgggatca caacgtcgtg gtctcgacga
    17941 cgagcggcgc cgaacagcgc gtcatcggcg gtttcggcac cacgaacggc aagttccgca
    18001 gcccgcacgg cctggccctg gacgcccagg acaacctgta tgtcgtcgac gcgggcaact
    18061 ggcgcgtgca ggtcttcgac aagaacggca acttcctgcg caagttcggg accaagggtt
    18121 ccggcctcgg acagttccag ggcgacatgc gaggcatcgc gatcaacaag gcacagggct
    18181 gggtctatgt ggtggacggc gagggcaaca aggtccagcg cttcgatctc aacgggaagc
    18241 cgctgaacaa gtggggctcg caaggctacg gacccggcca gttcgccgac ggtggccgcg
    18301 aggcgaccgt cgacaacgcc ggcaacctgt gggtcgccga catgccgaac ttccgggtgc
    18361 agaagttcag cccgaccggc aaattcctcg cggcctatcc caacacgccc cagcccccgc
    18421 cgctgggcgg cttcaacgct ccccgcgggg tggccgtggc caaagacggc acggtgggcg
    18481 tgagcgatac ctacaactgg cggatcgaga ccttcacggc gaccggcaac cccatcaagc
    18541 agtggggcaa gcgcggacgc gggccctacc agttcaacta cgcgcgcggg atcgccgaca
    18601 accccgtcaa caacgagtgg atcatcaccg acaccgacaa cggccggatc gtctcctaca
    18661 ccaaggacgg cacctaccgc tgggcctcgg gcacgaccgg ccgcgccaac ggccagttca
    18721 agaacccgca gggcctgacg gtcgcggcga atggttcgat ctatgtcgcc gactccaaca
    18781 atgcgcgggt ccaggtgctc aactccagcg gcgggttcgt gcgaaccttc gggacgaccg
    18841 cgcagttcgg ctatccgcgc ggcatcgccg tcgaccccaa ggaccagagc atctgggtca
    18901 ccgatatgac caaggccgcg gtcactcact ggagcccgac cggtgctttc ctgggcaagc
    18961 tgaagatcac caaggggacg gccgacaacc agctcgcgag ccccttcggg atcgccgtgg
    19021 acaatgagcg cgtctacgtc gccgacgtgc cggtgcaccg ggtcaaggtc ttcaccaaga
    19081 ccggccagtt cgtcaccacg atcgcgcagc gcggctacac caacggcaag ctcttccagc
    19141 cggcccacat ggacatctac aacgaccgtc tgtacatcgt cgaggagggc aacgagcgcg
    19201 tctcggtgtt caagctgaac acccgataac actccgacct cttagcgcag ccccggaccc
    19261 ctcgtgggga tccggggccg cgttttgccc aaatgtgatg cacctcacat tggacaggtg
    19321 cccgtgcgtg aatccccgcg tgcgaggcct attgacggct tcgtaaaccc accagtttcc
    19381 cctcgtcaca ctggtatatc gctgcggggc ccttgagccg tcaagcgggg cccggctagt
    19441 gtgcttccgc cctgatcggt cgatggcgcc gggtactcga cgcagccgcc gacgtggacc
    19501 atgacggttc ggatcagcgt gtccatgtcc ccccgccccc gcgaggcctg ccgtgcctcc
    19561 cgcactcccc cagtgcacca cgacgcccac ggagtagacc tgtgtcccga tccactcgag
    19621 ccctgaccgc tgcggtcgcg ctgaccaccg cagcggcccc cttcgtcctg accaccacgg
    19681 cccatgccgc cggcgacctg ttcgtggcca ccaccggcct cgacagcaac tccggcgcgg
    19741 cgacgtcacc gctgcggacc gtccaggcgg ccgtcaacaa ggccacgccc ggcaccacca
    19801 tccggatcat gcccggcagc tactacggca acgtcaagat caagacgtcc ggcaccgcca
    19861 gcgcccccat ccgcatcacc gcccagacgc ccggcaccgt gacgctgaca tacaactctc
    19921 gtccggagac ctgcagcaac agccagcccg ccggcgatcg gacgatcacg atcggtggcg
    19981 gcgccgacta ctggcagatc gacaacctaa agatccacaa cggcatctac atcagcgggc
    20041 agaaggccaa ctcggcatac agctggctca ccaacctggt caacaagctg gactggtcga
    20101 cccggcgcaa ggtgcccgga cacggcgcct acaacccgag tgcgctcggg ggcgtggtgc
    20161 cccacctgcg caaggtaacc ggcgttgccg acatcaacgc ggccgagggc aacaagatca
    20221 tcggcaacac gatgacgggc aggggcatct acggcgccct cgccagcaat ggcacgatca
    20281 gcggcaacac gatctccaag atcccctgcg gcatcggccc gggcatctgg atgatgacct
    20341 tttccaacaa caacaaggtc ttcggcaacg acatctccga tatcgccatc tcgcacgcgg
    20401 cgcactacat gcaggagggc atccgcgtcg gcagcgccgc cgactacaac gagatctacg
    20461 acaactacgt gcatgacctc cccggtgacg ggcgcggcat caacaccgac gtcgacggca
    20521 gctggaacca catccaccac aaccgggtcg ccaatgtcgc catcggctac aacgaccaga
    20581 tgtcgggttg gggcaaccgc tgggagtaca acacggtgac gaactatcgc gtctacggga
    20641 tgggcttccg gctcatggac gcccggctcg cccagccgtc caaggccagc agctccaacg
    20701 gctcgatcgt gcggtgcaac aacatcgtcg gtcccgtcgg cgaccgggcc aaggcgctcg
    20761 gtgtcggcgc gatgatgaac ggcaagttcc agagcaacaa catgcccctg atctggatca
    20821 gcaagaacat gcagacctac tggagcgccc agggcaacac gtggaacggc tccagcgcgt
    20881 tgccgagcaa gaccccgacg ctgtccagcg ccggctgctg agcgctcccg gcccacgagc
    20941 gaagccgccg ccccccgagg gggacggcgg cttcgctcgt gggccggggt gattcggcgg
    21001 cgggctactt ggggacggcc ggggaaccgt tccaggtgtt gccataggac gaccagtacc
    21061 gggtcaggtt cttgctgatc cagaaggtgt tgaagttgtt gccggagaag gtgcccttca
    21121 tcatgccgcc ggcgcccatc gccttggcac ccgagcgcgc cgggtgcaag gcgacattgc
    21181 cggaggagac aacgccgttg gtgctggtgc tcatcgaggg gagcgacaaa ctggcatcca
    21241 tcagccggta gccgtagccg tactggcggt agttggtgac ggtgttgttg cgccaccggt
    21301 tgttccagcc ggccatctgg tcgttgtagc cgatggcgac attggtcgcg aagttcttct
    21361 cgaaggtgtt gtagctggag tcgacatcgg tgttgaacgc ccggccgtca ccttgcaggt
    21421 cgtggacctt gttgttggtg atcttgttgt agttcgcggc cgatccgaac cggatgccct
    21481 cttgcatgta gtgggcggcg cgcgagatgg cgatatcggt gacgtcgttg ccggtcacgg
    21541 tccagaagtt gctgtggttc atgatccaca cgcccggacc ggagccgcag atgatcttcg
    21601 agatccggtt gccctgcacc acgcccgacg tggtcagcgt cgcgaagatc ccgcggccgg
    21661 tgatcgtgtt gtcgatgaac ttcactccga cgaccgggtc gagcgcggtg ttcagcgcct
    21721 tggcgaggta cgggatcgcg ttgcgggcgg ccgtcgggtt gtaggacccg gcgccgggca
    21781 ccgcgcgccg cggctgccag atcttcttct tcaccaaggc ggcatgccac gagaagacct
    21841 tgcccgagcc cttgcccgac aggtaggcgc cgtggtggat gttcaggccc ttgaaggtcc
    21901 agtagtcggc acccgagagc atcttgatcg tgcgccgcgg ggagggggcg ctgctgtagc
    21961 aggagtccgg cgactggttg gacgtcaagg tgacggcgcc gtcacccgcc ggcgtcaccg
    22021 tgatgggagc cgaggcggtg ccggagttct tgatccggat ctcaccgaca taggtgccgc
    22081 cgtgcacgcg gatcgtcgtg ccgggcttgg cccggtccgc cgccgcctgg acggtgcgca
    22141 gtggggacgt ggcggtgccg gcgttgccgt cggatccgcg ggtggagacg tggatctcgc
    22201 tgccggcggc gccggccggt gccgcggcga gaaccgcggc gccggtgacg gcagcgccca
    22261 gggcgagggt gagggtcttg tggggggtgg tcatcgattc tcctggtcag cagtggctcg
    22321 gggcgaaggt ggcgggcggc ggggtcggct tgccgttgaa ggtgttgccg tacttgcccc
    22381 agtacctctg caccgacttg gcgaccttgg cggtcgtcca ggcgtttccg ttgtaggtgc
    22441 cgtacatcgt gccgccgacg ccgaaggact tggccgtccc gatcgggcga ctcgcgacat
    22501 tgcaggacat gttgatcttg ttggcgctgg agttgtagga gggcagcgtg agcttgccgt
    22561 ccatcagccg cacgccgtag ccgtaggtgc ggaaggaggt gacggtgttg ctcttccaca
    22621 cattgcccca gccggacatc tggtcgttgt agccgatggc gacattggtg gcgcggttgc
    22681 cggtgatggt gttccagctg ctgtcgacgt cggtgttgta ggcgcggccg tcaccgggca
    22741 ggtcgtggac gttgttaccc gtgaccttgt tgtagttgga cgcggtgccc atgcggatgc
    22801 cttcctgcat gaagtgcttc cacgtcgagg cggcgatgcg ggacacgtcg ttcttggtga
    22861 cctgccagcc gttggagaag gtgatcagcc agatgccggg gccggtgccg caggccaggt
    22921 cgctgatctt gttgccggag gccacgccgt aggagttcat gcggaagaag agccccttct
    22981 tggtgaccgt gttgttcagg aacttcaggc catcgttgga atccaggtcc tggtcgcctg
    23041 tgacggtgcg caggtaggga accacctgcc tggccgcggt cgggttgtag gaaccggcgc
    23101 caggcacctt ccggcgcgtc tgccagttgc cctggtcgac gagcctgctg tgccaggtgt
    23161 aggccgaacc cgcgtccttg ccggtcgagt agacgccacc gacgatgttg agaccctgga
    23221 aggtccagta gtccgagccc gagatgatca ggattgtgcg ctgaccgtcg ggctggctgt
    23281 tgttgcacgc gggcatcggc agattggcgg tcaaggtgac ggcgccatcg ccatacggca
    23341 tgacggtgat cggcgcgctc gccgtgcccg acttgcggat cttcagctgc tggttgtagg
    23401 tgcccttgtg gatcttgatg gtcgtgcctg cggcagcctt gtcgatggcg gcctggacgg
    23461 tcttgagggg cttgcccggt gcgccgctgt tggcgtcgtt gccggtggtt gacacctcca
    23521 ggacggtgcc ggcggcggtg gccgggccgg ccccggcggc gaccgcaccc gcgagcacga
    23581 cggtggcggc cagcccggat gacatcaggc gaacagaggg ggtggttttc atggtggggt
    23641 tctcactcgt gcgacgacgg cggtcagtcg cgcccgcgat tttccacggc ccatactccg
    23701 cggcgaactg ccccgagaca ggggccagca ccaggcgtga cgtggcgagt gtagatcacc
    23761 attggataac gaggccaatt cgcccgggtc acggacgatt gggttggctg ccggcgcgcg
    23821 gcggttcagc cggcggtgac gggcgagccg accaccgctt cgccggcttt gagagcgccc
    23881 tcgcggtcgg tggcggccag ctcgatcaac accggatcgc gcggcgtcga gcccgcgggc
    23941 acgctccccg cggggtcggc ggccgagcgg gcaccggcgc cggtgatccg caggctgccc
    24001 tcgccgtcgg gccacagcac gagcaggctg ccgctcggcg cgacgagcag tatgccggtg
    24061 cccccgctcg tgcgtacgcc ggcgacgtcg gtgctgtcct tggcgtactt ggcgtaggcg
    24121 gcatacacct cccccgccgg agtcgacgca ccctcgggcg tgaccaggcc gcggaagatc
    24181 tccaccttcg tcgcgccgcc gggggtgtag gccagcggca ggtagacgat ggggctgacc
    24241 tgctcccgca tgagcgcgcc gagcaggcga gccgtctcgg cgccgtggat ctgctcggtg
    24301 tagttgtcgc ccggccaggc ggtgccgatc tcccagcccc cgatcgggat gcgcacggtc
    24361 aggtgccggc gtatgtagtc gagcaggtcg ggcagcaggt ccgggttctc gtagaaatgc
    24421 agctggtatg acgtgacggc gccggagttg atcgccgccc agttcgccgc gaccatggcg
    24481 cggatgcgct tggcccgccc gtcgctcagc aggctgcgca gggcgtcgac ggaatcgacc
    24541 atgccgaagc gggcctgcgg gccctcgttg cgacggccga accactgctg gtagagggcg
    24601 agggcttcct gctccttgcc ggcgtccatc agctcaccgg cgagctcgat gccgttgccg
    24661 gtgctggaga tacccgcgtc gaggatcgtc gcgtcggggt cgccggcctt gatcgcctcg
    24721 ctcgccatcg tgaccagccg ggtgtactcc tcgggggtgc ccgaccagaa gttgttggcg
    24781 tcgacctcgt tctcgatggc ccacgacttc acaccgcggc ccgaatagag cttcaccatc
    24841 gtcgtgacga actgctggta tgccgcgggg tcgtcgggga atgtgctcgg gctcttgcgc
    24901 gtgccctcgg ccgggtcgag ttccttgttg ccgccgccgc agttgccgac gcggatcttg
    24961 agcatcagcc ggtagcccag ggcctgggcc tggccgagga tgcgctcgac ctgggcgaag
    25021 cgggtgccct cgggccggtc gcgcagggcg caccattcga cctcgaagaa ggtctcaccc
    25081 cacccgacct gctgggcgta ggccagcggt ggcttgagat tccagttcca cttgatgccg
    25141 aggtcgacct tgccggtctc catcaacacc ggcgggacga cgcccatcgt gcccgaagcc
    25201 atcgtcgccg gcgtggtggc gcgaccggtc gggccggcgc cgtcgtcgca ggcgctcgcg
    25261 gacagcacca gtgtcgccgc ggccagggtg gccagggcct tcttgaccat gtcggtcagc
    25321 cctccgcgga gcccgcggcg agggagcggg ggtagccgta gcgcgtcagc agcggcgtgg
    25381 tcaaggccgt cacgacggcg gcctccttgc ggtccatcgc gccggcccat tcgttgtcga
    25441 gtcgcacagc agtaggaccg atcgacatgc gcgcagggtt cccggcgacg gtgtggttcg
    25501 cgggcaggtc gagccggccc tcgtcgtcga cgagacggtc gacacccgtc aggccgagcg
    25561 cgtcggcgag gacctgcagc gactcgcggg ggtgcgtcgc gaagctctcg tagcgcatcc
    25621 gcacgtagtg cggcgaacca cgccaggccc gctcgaggcc ggcgttccac gccgtccaca
    25681 aggtggccga cttcacgacg ccgcggcgct ccatcaaccc gccggagtcg ttcttctcct
    25741 tcttgcgctg ccacgagaag gcggcggcgc gcgggtcgcg cacgaggtgc acgacatcca
    25801 gggcgagccc gggcaccccg gcgagcaggg cggcatacgt cggaagcttg gaagagtcga
    25861 cgatcaccga cttgccggag acggtgccga tcgcctcata cagcggcgcg aggacggcgg
    25921 ccagcgcctc gggggcgctg gagcgctccg cgggggtgcg ggccaggcgc ggcagcgtgc
    25981 gcagctgggt gtacgccgtc agctgctggt gcatctgctg ggcgtcgggc accgcggcgc
    26041 cgtatgccgt gcgcagcacc tcctgccaaa aggggcagtc ggcgaaggcg gccccgcagc
    26101 cgcacagccg gttctcggtg atgccgcgct cccacaggta acgcaactcg ccgacgctga
    26161 ccgcatcgcc caggccgccg agagcgttgg cgacgacggt gctaccgctg cgcccggtcc
    26221 ccccgacgaa cacgacccgg accgaatcgc cgttcgtcat cggctttcgc ccttcagttc
    26281 tcgtaggtct cgcggcggac ccgctcacgc gcgatcagct cgtcgtcgct gccctcacgg
    26341 ctgcggcgca gcacctcggg gatggcgagg aggaggaccg ccagggcgag gccgatgagc
    26401 gcggccgaga tggcgcgacg caggatctcg gtggtccacg gcaactgggt gacctcggaa
    26461 ccgatcgact cggtgatgct cgcggcctga cgcgactcga tgtcggtctt ggctgccgag
    26521 cgctgggcga gggcggcgcg ggcgttgtcg acggcaaact gcggcgcggt gaactcggcc
    26581 tgcgcgtcgg cgacctcggt gacccgcttc tcggcggcct tgagggcgtc ctcgatcggc
    26641 ttggtcgtca ccgtgtcggg gcggcccttg gcagtggcga gcgcgacctt gagcgaggtc
    26701 acctcggagg tcgcggtctg gtattcgcgg tcggggttgg tcaggccggt ggacttctgc
    26761 accttggcca gctcggccat cgcggtgtcc aagcggttct tggcctcgtc ctcggcgacc
    26821 tgggcctggc gcagcgacgg cgcgaacgcc tgctcctgca cggccgaggg aatggcctgc
    26881 atgaccttct cgacgattgc ctggtcggtg tcggcgaagg tcacgtggac cagctcggtg
    26941 tcggcgatgc ggctggccga gatgccgtcg cggatgcgct tgaccggggt gccggccgtc
    27001 ttggcgatct tggacaggac ctcatcggag gtggtcgcgt tgacgagcga cccggcgacc
    27061 aggttgatcg aggcgacggt ggggtcgccg accgggttgg tcgcacgcag gtcgacggag
    27121 ctggtgtact tcgtgccgct cggggcgttc aggcccagga ccagcagcag cgccgcgagc
    27181 gggatgatca ccagcagcca caggcggcga ccaaggcggg cgagcagttc gcgaagttcc
    27241 atagtgaggt gtatccgact ctttcgacag gtacgggtgc agtaggcaac tgagtctcgg
    27301 cggcgccagc gggagctagc ctgcgggctg tcgcttgcgc gacggcgcgc caaggcccag
    27361 cggccacaag ggactacgat acaccccggc catcggggga cttccccgcc atgccgcttc
    27421 gcgcccgcac tgtggaaggt cgcctggtca tcgtgacgtc cgatgcacgc cctcagcccc
    27481 cgctcttcat cgtcggtgca ccgcgctccg gcacgagcct ggtctatcgg gcgctcgccc
    27541 tgcaccccga tgcctcgtgg atcaacaact accagcggcg gctgcccggt gtcagcgcat
    27601 tggcggtcct gaaccggctg ggcgcgcgcc ggctggatca gcggcgggcg gtgtggttcg
    27661 gcgcgtcggg cgacaacgcc taccgctaca acacctcgcg gtcgctggcc gagcgcttct
    27721 tcccgcagcc ggtcgagggt gagcccgtct tcgagcactg cgacgtgccg gaggcgtggg
    27781 acgggtcgcc ggtcaccgac aagcagcggc gcctgccggg cctgctggcg cgcacgacgg
    27841 ggcgcagcgg cgggcgggtg ctgctgagca agcggatcgg ccacaaccgc cggatcgggt
    27901 tgctgcacaa gctgattcct caggcacgct tcttggacgt gacccgcgac ggccgggctg
    27961 tcgcctattc gctgagccgg gtggactggt ggcccgagat gcccgtgtgg tggtgggacg
    28021 accgcacccc gcaggagtgg gcagccgagg gcaaggaccc gctcgagttg tgcgcgcggc
    28081 actgggtcga agagacgcgg gtgatcgagg aggggcttgc gtcggtgccc gccgggtcgg
    28141 tgctgcggat ccggtatgag gacctggtcg ccgatccgtc gcgtgtcctg ggcgaggtgg
    28201 ccgccttcgc cggtctcgat cccgacaacg ccgtctggca ggcggagctg tcgaagatct
    28261 ccttccccga caagaacgcc gcctggaacg cagagatccc ggccgagcaa cgggcggccc
    28321 tggccgtcat ggacgaccaa ctcaacacca tgggataccg ctgatgacct ccttgccccc
    28381 cggcttcatc ctcggcacgg gccgctgcgg ctccaccctc gtgcacgaac tcatcgcccg
    28441 gcatcccgcg accggcttcg tcaccaatct cgacgacctc ggcatccggc agggcaacgc
    28501 gcagcagctg accctgtggc gccggctccc ggccgcggtg tcggagaagg gccgagcccg
    28561 gctggccccg agcgagggct atcgcgtgct cgcccgcgag gtctccccca tgcttgtcga
    28621 cccggcccag gatgtgacgt ccgccgacct gacgccctgg ctcgagaagc ggctgcacgc
    28681 cttcgtcgag aagcggatgt ccggtctgga gtccgccgag atcttcctgc acaagttcac
    28741 cggctggccg cgggcgtcgc tgatcgccgc cgccttcccc gacgcccggt tcgtgcacat
    28801 cgtgcgggac gggcgggccg tcgcgaactc gtggctgcag atggactggt ggaagggcca
    28861 cctcggcccc gccggttggc acttcgggcc cctctcgccc gaactcgacg aggcctggga
    28921 cgccgccggg cgcacccagc ccgcgctcgc ggcgatcgcc tggtcgatcc tggtcgaccg
    28981 gcacgaggag tgcgccgccg aactgggaga tcgctggctc accgtgcgct acgaggatgt
    29041 cctcgaggac tcccggaccc acctgacccg catcctcgaa cacctcggcc tgacctggac
    29101 cgacgccttc gagtccggca tctcccgcta cagcctctcc tccggccgca aggcggccta
    29161 cctgcaggac ctcacccccg cccaggtcgc caccatggag tccctcatcg gcgaccagct
    29221 ggcccgctac ggctaccgct gagcccgcgc gccggcaaca catgacgagc ctccacaccc
    29281 actgctgggg gtgaccaccc cggtggcgcg ccgccacgac acctcgagga aaacgaaccg
    29341 gggcgaacca cggtaggacg ccacgagcag ccggccccta cggaactcga ccgcggcggg
    29401 gccgacgatg cccgcgagac gggggcgcga ggaacgagcg cacccgccga gggggaggag
    29461 gagaccccgc gaaagggctc aatacgcgcc ttcggccttg acgacggcgc ggacggtgcg
    29521 catgaggatc atgacgtcga ggacgggtga ccagttgtcg acgtaccaga ggtcgaggcg
    29581 cagggactct tcccagttca ggtcggagcg tccggagatc tgccacatgc cggtcaggcc
    29641 ggggcgcacg cgcagccggc gcagggcgtc gggctcgtag gcgtcgacct cgcgcggcag
    29701 cggcgggcgg ggcccgacga gggacatctc gccgcggagg acgttgatga gctgcgggag
    29761 ttcgtccagg gacaggcgac gcaggatgcg gccgggcttg gtgatccggg ggtcctgctt
    29821 gtgtttgaag agcatctcgt tgacctgctg gtgcgccgac agttcaccgg agagcaggcg
    29881 ctgctcggcg tcgacaacca tggtccggaa tttcaccatc gggaaggtgc ggccgttctc
    29941 gccgacccgc tgctggatga agaagaccgg cccgcggtcg tgcagcttga tcgacaccgc
    30001 gatgaacagc cataccggca tcgacaccag cgcgatcagg ctggcgcaca cgacgtcgat
    30061 gacccgtttg gcgaaggcgt cgaagccgcc gatggtcggg ccttcgacgt gcagcaggga
    30121 catcccggcg gcgggccgca gagtcaggcg aggggccgcg acctcgttga tgccggggtg
    30181 gacgaacagt tcgacgccga tctcggacag ggcccagccg aggcggcgca gcgccttccc
    30241 cgagaaagag gcgtcggagg cgacgacgac ggcctcggcg ccggtgtcgc ggaccgcgag
    30301 gagcgcggtc tcggggccgc cgtagacctc gacatcggcg acccgtttgg ggtcgggcag
    30361 gtcggatgcg caggcgccga cgaccttcat cccggtcgac atcgggtcgt tctgaatgga
    30421 ggcgatcatg tcgtggaccg ccaggccgcg cccgacgacg atggttggct gcagcgcgtg
    30481 gccgcgggtt cgcgctctgg cgagccggcc gcgaaggatg cgccgggcca ggacggtgag
    30541 caggaaggtc aggggcacgg cgacgaagaa cagcaggcgc gacatccgcc agttcaagat
    30601 gacgagggtg accgacagca acgcgagcag gatcaccaag gcccgcccga tgcttcggta
    30661 ctcatccagc ccgatgccca gggtgttgac gttgtaggca ccggagcgac cgcataccag
    30721 cacccacacg accgggacga cggcggtcag cagcgcgacc cgcagattgg ggaccccctg
    30781 atagcgccac agggccgcga cgaagcaggc cagcagggcc gcgaggaagt cactgacgat
    30841 gacccgattg cgataccggg tccgccaccc ctggcccggc ccttccttgt ccacattggg
    30901 gagcatccag tccggggtat gccagtgcac ggactggctg tgcggcctgg gtacggtcgt
    30961 ctgcaacgac ggattcgagt cagcgtcctg ttggtgctga tccgcctgtg ctgccccgct
    31021 cacactttcc tcccgactct cacctgcggt ctttcgcgcc gctcggtgat acccatgtga
    31081 ccagtggttc tgaggaacgg atttgttccc tgggggaagg taccgcaaga gaccccatta
    31141 ccacgagttt cccccaaact aagaccgcca cgccgcccca ctgaaccggc gcgcggttcg
    31201 cggagcgacg tggcggaaga ccatgtggat caggcctcgg catctgcctc ggcgccggcc
    31261 ttcgcctcgg cgggggcctc ctcggccgcc tcggctgcct cctcggccgg ctcttcctgc
    31321 tcgatgccgg cctcagcctc ggccagttcg gcgtccagcg cctcctggga gatcatcgcc
    31381 gtgacgttga ccacgaggct ctccgggtcg gtcgccaagg tggcgcccgc cggcatggtc
    31441 aagcccgcgg cgaggatctg ggcgccggcc tcgaggccct cgatggacac ctcgatgttc
    31501 tcggggatgt tggtggcctc gacctcgatc tgcacggtcg ggctctcgac gttgacgacg
    31561 gtctcggccg ccgcctcgcc gacggtgtgg accgggacgt cgacggtgac cttctcgccc
    31621 ttgcggacga caaccaggtc gacgtgctcg atgacgggct tgatcgggtc gcgctgcacg
    31681 tccttggcca gggccagctg ctcgtttccg tcgatgacga gggtgagcag ggcgttggcg
    31741 tgcttgaggg ccatcatcgt gtcgtggccg ggcatggtga tgtggatcgg gtcggtgccg
    31801 tggccgtaga cgacggcggg caccttgccg gcgcggcgga cgcggcgcgc cgcgcccttg
    31861 ccgaactcgg tgcggagctc gacgtcaaga cggttctcgg tggacaactg cttctcctca
    31921 aggcgggtgg cggccttggg ggaggtcgcc atggggatcg gtatgcggtg gccccgggtc
    31981 gctggtcgct gcgggggcgc gacatcatcg ggcaccaccg cgtcgatcac gggcggcgga
    32041 tcgccgcccc tcgccgaggc atcgttccta gagtagtcgc ctcatcccac cgggacgaaa
    32101 tccgcgagaa gcggacgcgg cgcggccgag acgggcggga cgggcgggac ggggacgcga
    32161 ggacgaagcg gccccgccga ggcgagaagg agacggtgcg cagagtgctt acagctcgtc
    32221 gaagaggctc gtgaccgagc cgtcctcgaa gaccgcgcgg actgcctcgg cgatgagcgg
    32281 ggcgacggac aggacggtca gcttgtcgaa gtgctgctcg ggggtcaggg gcagggtgtt
    32341 ggtgacgatg acttcctggg cgctggagcc ggcgagtttc tcgacggcgg ggtcggagaa
    32401 gatcgcgtgg gtggcggcga tgacgacgcc ggcggcgccg gcctccatgc aggcctcggc
    32461 ggccttcacg atggtgccgc cggtgtcgat catgtcgtcg acgacgatgc acacgcggcc
    32521 ggcaacgtcg ccgacgaccc ggttggcgac cgagacattg ggccggtgga tgtcgcgggt
    32581 cttgtggatg aaggccaggg gggcgttgcc gaggcggcgc gaccagtgtt cggcgacctt
    32641 gatgcggccg gcgtcgggcg agacgatcgt caggtcctgg cgggcatact tctcgccgac
    32701 atacgccgac agcaccggca gggccatcag gtggtcgacg gggccgttga agaagccctg
    32761 gatctggtcg gtgtgcaggt cgaccgccat cagccggttg gcgccggcgg tcttgaacag
    32821 gtcggcgacg aggcgggccg agatgggctc gcggccgcgg tgtttcttgt cctgccgggc
    32881 atacccgtag aacggcagca cgaccgagat ccgcttggcg gaggcccgct tcagcgcatc
    32941 gaccatgatc agctgctcca tgatcgcctc gttgatcggg gcggcgtgcg actggaagac
    33001 gaaggcgtcg cacccgcgga ccgactcctc ataacgcacg aagatctcgc cattggcgaa
    33061 gtcgtagatc tgtgtgggca ccagttcggt gccggcaagt tcggcgacct cctcggcgag
    33121 atcccggccg acccggccgc tgaagatcat caggttcttg cgggtcttct tggtgatgcc
    33181 ggtcatgcct ggcgctcctc cccctcgcgc gggccgggat gcccgccggg atccccggcc
    33241 ggcgccgggt tctggtccag ggcggtctgc gccgccgccg cggtcttggt gccgccgcgg
    33301 gcccgctcga cccagccggg gatattgcgc tggcgggcgc gggcgacggc gatctcgccg
    33361 ggcgcgacat cgttgacgag tgccgagccg gcggcgacat acgccccgtc ggccacgctg
    33421 accggcgaga cgatgacggt gtgcgagccg acgaagctgt gccggccgac gaaggagtgg
    33481 tgcttggcca cgccgtcgta gttggcgaag atggtgccgg cgccgatatt ggcgccctcc
    33541 ccgatcgtcg cgtcgccgca gtaggtcagg tgcggcacct tggcacccgg cccgatcttg
    33601 gcgttcttgg tctcgacgaa accaccgatc ttgccctggg ggccgagttc ggtgccggcg
    33661 cgcaggtagc tgtaggggcc cacggtcgcc ccctccccca cgacggagaa ctccacctcg
    33721 gcgcggcgca catgagccca ggcgccgatc tcgctgtcgc ggatggtcac ctccggcccg
    33781 atcgtcgccc cctcgccgat cgtggttgcg ccgagcagtt gcgtgcccgg ctggatcgtc
    33841 acatcggtgc cgatggtgac gtcgacgtcg atccacgtcg aggccgggtc gacgatcgtg
    33901 acgccggcac gcatccacct ctcagtctgg cggcggctga ggacggcgcc gagggtggcg
    33961 agctggacgc ggtcgttgac gccctcggtc tggatgacgt cggagatctg gaaggccgag
    34021 acattgccgc cgtcgccgcg cgcgagcgcg aggacgtcgg tgaggtattt ctcgccctgc
    34081 gcattctcgg tgcccacgcg cgcgagattg gtgcgcagca cgtgcgcgtc gaatgcatag
    34141 attcccgagt tgatctcgcg gatctgcagc tgctcgggcg tgcagtcctt gtgttcgacg
    34201 atggcggcga cgtgaccgtc ggcgtcgcgc acgatccggc cgtatccggt ggggtccggg
    34261 gcgtcgccgg tcagcaccgt gatgacgctg tcgctctggg tatgccgctc cagcagcgcg
    34321 ttcagcgtgt caccggtgag gaggggcacg tccccgtagg tgacgaggat tgtgccgtcg
    34381 aggtcggcgg gcagcggctc gaggccgcac tcggcggccc gcccggtgcc cttgacctcg
    34441 tcctggtccg cgatgatcgc gtcggggtcg acctgcgcta tgtgctcggc gacgagatcg
    34501 cgttggtggc gcacgacgac cgagaggtat gccggcccgg tcgcccgagc ggccgcgatc
    34561 gcgtgcccga gcaggctgcg cccgccgatg cggtgcagca ccttcggggt tgcggacttc
    34621 atccgggttc cctcaccggc ggcgagaatg acaacggcgg cgggggcttc ggaggtcacg
    34681 accacatcgt atgtggcgcc ctcgcctccc acgtccttcg ccgtgtcaac ccgggcgccg
    34741 gatcgcgcgt gctcgatgtg ccggacgcgt caaccggcgc cggccggacg ggcagtgacg
    34801 gaggagacca tgaagcacct gacatctcga tggggtcgaa tcggcatctc gacggctttc
    34861 gccgcgcaac tcgccctcgt cgccgcgttg tgcggctgcg gcaccgtcgg ctccgctccc
    34921 ggcacgcagt catccgggac gcagtcatac agcacgcagg agtcctcccc cactcaggag
    34981 acagcgaccg ggacttcgtc gaccccgccc agcaccgcct cctccacaca ccccgccacc
    35041 accacagtgg ccggggagag cgtgacgatc ttcctgccca acgacaagct cgccggcgcc
    35101 ggcgacttct gctcgctcgt gtatccgctg cagcgcccgg tgccggccgg ggcggatcgc
    35161 gccgaggtcg cccttcggca gctcttggcc ggacccaccg atgccgaacg cgcacaggga
    35221 tacggcggct ggttctcgac ggcgaccgcg aacgacctga tcggggtgca ggtgcggggg
    35281 cagacggcat acgtcgatct gcgcgacttc tccgcgacga tccccaacgc gagcacctcg
    35341 tgcggaagct cgatcctgct gcaccagttg gacgcgacga cgagcgccgc caccggtgcc
    35401 gacgaggtcc gatacgcctt cgcgggggat ccggcggcgt tctggaactg gctgcaggtc
    35461 ggctgccccg ccggtggcga cgaccgctgc gacccggcac cgttgcggtg agctccccgg
    35521 gtaggaatcg aacctacgtc gctaatcctg attcaaagtc aggcgggccc taccagcaga
    35581 ccaaccgggg atcgccgcgc cgtgagcggg caggacaagg gtaatcgagc ctgcacaatg
    35641 gaatggtgag tgccagcgag ccgcgcggtc ggcatccatc cgggcgcccc cggatgaccg
    35701 gggtgcagcg acgtgagcaa ctgatcgccg tgggccggaa gtcgttcgcg gagaagggtt
    35761 tcgaaggctc gagcgtcgag gagatcgcct cccgcgccaa ggtgtccaag cccgtggtct
    35821 acgagcactt cggcggcaag gaggggctgt atgccgtcgt cgtcgaccgg gagatcgccg
    35881 acctgaccgc acgggtcacc gacgcgttga gttccgaagg gtcggcccgc accctgatcg
    35941 agcgggccgc gctcgccctg atcggctatg tcgaggagga gaccgacggc ttccggatcc
    36001 tcgtgcgcga ctcgccgccc gggcagcaga cgggttcgct cgcgagcctg atgagcgatg
    36061 tcgcgacgca ggtcgagcat ctgctcgcgg cgcagttcaa ggcccgcggg ctggaggcca
    36121 agaccgcgcc catgtatgcg cagatgctcg tcggcatggt cgcgctgacc gggggctggt
    36181 ggatggattc caaccgcaag ttctcgcgtg aagatgtggc cgctcatgtc gtcaatctcg
    36241 cctggaacgg gttgacgggc ctggagaaaa acccgacgct ccacgcctga ggggttacct
    36301 cttgtggccg acggcgaaga tccgccggaa gggcaggatc accccggcgg gggtgcgggg
    36361 gtaggcctcg cgcaggcggg cggcatacgc gtcgacgaaa gcggcccgct cgtgctcctc
    36421 ggtcagcaga tcgatgaccg gccgcagacc ggtgcccttg acccattcga ggaccggatt
    36481 ctcctgtgcc ccttcgggat cgagcacatg cagataggtc gtctcccagg cgtccacgct
    36541 cagcccctcg cgcgccagca cctgcagata ggtcgccggc gcggccgaac cgccgcgttt
    36601 gagggcggcc tcgagttcgc cggcacgcgg ctgggtgatc gcgacctcgc gcatcaggcg
    36661 gtgactggcg gcgtcgtggt tgcccggcac ctgcatcgcg aaccagccgc ccgcggccag
    36721 cgcggctgtc caggcgggga tgagttcgag atgccgaggg atccactgca aggtggcatt
    36781 ggtgatcacg acatccgggg ccgcgcccag gctgccaatg tcccactgcg cgaggtcgcc
    36841 ctggatccat tcgacgcggc cgtcggcgtc cagcgcccgg gccgcgtcga gcatctgggc
    36901 cgaggagtcg acgccgacga ttcgcgcggc cggccagcgc tgcgccaggg cgagggtcag
    36961 gggcccgttg ccgcacccga ggtcgacgac caggccgggc tccgcggcgc cgacacgcgc
    37021 cgccaactcg tggaacggac gactgcgttc gtacgcgaac ttggcgtatt ggcccgggtc
    37081 ccagcgcgtg gtcatcggcc tctccctcgt atggcaagta tcttgacgtc aagatacttc
    37141 tcacatatct cgacgtcaag atacttgacg cctagagtgg acggcgtgaa cgtgccaccc
    37201 gagccggcgc cggccacgga cgacgtcgat gccatcgtcg ccgcctggca ccgggagcga
    37261 cccgacctcg acctgacgcc cctgcacgtg ctctcccgtg tcacccggct ggcccgccgc
    37321 ctcgacctgg cccggcgcga ggccttctcc gcggttgcgc tcgagccgtg ggagttcgac
    37381 gtgctcagcg cattgcggcg ggccgggcgg ccataccgcc tctcccccgg ccagctcgtc
    37441 gaggccaccc tcgtcaccag cggcaccatg accaaccgcg tcgaccgtct ccaagagcgc
    37501 ggcctcgtgc gccgcctgcc ctcgcccacc gaccgccgcg gcgtgctcgt ggagctgacg
    37561 cgcgaaggcg cccagaccgt cgacggcgca ctgacggcgt tgctggagaa ggaacgggga
    37621 ctgctcaccg gtctccccgc ggccgaccag gcgcagctcg ccgacgccct tcgcgccctc
    37681 tcgaccgcca tcgaccctcg ctgagtccgg catccggcga cctgtggctc gccggccgtc
    37741 ggcccgcttg cgaacgggtc agccggcgat gtccgccgcg gcgagccagg cctcctcgag
    37801 gtcgacgacc tggtcggcca cggcgcgcag gtcggcggcg agggtcatca tcgcctggtg
    37861 gtcgctggcc cgggctgcca tctcctcgtg gaggcggtcg gccttctccg acaggcgctg
    37921 caactgcttc tcgatgcggg cgatctcctt gcgcgcctcg cgcacctgcg cggggctggg
    37981 gccggcggcc ggggccggcg cgctcgcggg accgggcggc ttaccgccgg ccgcggcggg
    38041 gcgagcgggc agggtatgcc gcagctcgag gtactggtcc acgccaccgg gcaggtcgcg
    38101 cagccgcccg tcgccgagga gggcgagctg gcggtcggtg atccgctcca ggaggtaacg
    38161 gtcgtgggag acgacgacga gggtgccggc ccagccgtcg agcaggtcct ccagtgccgt
    38221 cagggtgtcg atgtcgaggt cgttggtcgg ctcgtcgagc agcaggacgt tgggttcgtc
    38281 gagcaggacg cgcaggatct gcaggcgccg ccgctcgccg ccggacaggt caccgacgcg
    38341 cgcctgctgc cgcgggccgg ggaagccgag gcgcttggcc aggctggagg cgctgaccgg
    38401 cttgccgtcg aggatggtcg aggcgcgcac ctgttcgatc gcctcgatga ccttcagccc
    38461 ggccagcttg tccagttcgc ggacctcctg gctgacgaca ccgatccgca cggtgacccc
    38521 gaccttgcgc ttgccggagt cgagcggctg gcggccggcg agcacattga ggacggtcga
    38581 cttgccggcg ccgttgacgc cgacgagccc gatccgctcc cccggcgcca gctgccaggt
    38641 cacccgcgcg aggatcggca ggtcgccgaa tgccagggac gcgtcgtgca ggtcgatgac
    38701 gtccttgccg agcctggcgg tcgcgaagcg catcagttcc accgaatcgc gcggcggcgg
    38761 ctcgtcgtcg atcagtgccg aggcggcgtc gaggcggaac ttcggtttgc tcgtccgggc
    38821 gggggcgccg cgccgcagcc aggcaagttc cttgcgcagc aggttgtttc gccgctccgc
    38881 ggtcacctgc gcgatgcggt cgcgctccgc tcgagccagg acgtatgcgg catacccacc
    38941 ctcgtaggac tccacccgtc cgccgacgac ctcccaggtc tcggtggcca ccgcgtcgag
    39001 gaaccaccga tcgtgggtga cgaccacgag ggcccggtcg gggcgcgccc aggtgcccgc
    39061 gaggtatgcc gcgagccacg ccacgccctc cacgtccagg tggttggtgg gttcgtcgag
    39121 gagcagcagg tcggcgtcgg cgacgagggc ggcggcgagg gcgaggcggc ggcgttcgcc
    39181 gccggacatc gacccgacga cggcgtccag tccgccgagc gcgccggcgc ggtcgtcacc
    39241 gagcaggccc gtgagcactt cgcggatgcg cggctggctc gcccactcgt gctcgtccag
    39301 ctcgccgagc acgacctggc gcaccgtggc ggcggggtcg agggtgtcgt cctgggcgac
    39361 ctgcgcgatg cgcagggagc cgttgtgcgt gatgcggccg gagtcgatgc ggcgggtctt
    39421 ggccagcacc ccgaggaggg tcgacttgcc tccgccgttg gcgccgacga ccccgacccg
    39481 gtcgccggtg ttgatgccga gcgagatgtc gtcgaggacg gcgacaatcc ccgagtgaac
    39541 ggtgacccgt tcgatattga cgagattggc catcagcggc aggtcgccac ctcgaccgga
    39601 cccacggcgg ccggcccatg agccttgcgc acatcggcgg ccgccccgga cgcggtcaag
    39661 gacacggcca ggtcgatggc cgactcggcg tcccggcaca agaaggccag cgtcggaccg
    39721 gagccggaca cgatgccggc cagcgcgccg aacgacaggc cggcctcgag gaccccggcc
    39781 agggcgggct gcatggacag ggcggcttct tgcaggtcgt tgcccatgcc gtccgcgact
    39841 gcctcggcat tgccgaagct cagggccgcg agcaggtcgg cgctcggctc gggctgcggc
    39901 accggcgcgg caccgcggag gcggtcgcac tcggcataca cggccggcgt ggacaacccc
    39961 tcgtcggaga gggcgaacac ccagtgatag gtgccccgcg tcgcggtcgg caccacgcgt
    40021 tcgccccggc cgccgcccaa ggcggtgccg ccggcgagga ggaaggggat atcgctgccc
    40081 aaaccggccg cgatcgcggc cagctcggcc ggcgacaggt cgagccccca cagccggtgg
    40141 cacgcgagga gggcaccggc agcgtcggcc gagccgccgg ccataccgcc ggcgaccggg
    40201 atctccttgg tgacccggat ccggaccggc cggtcgatgc cggcggcatc ggcgagcgcg
    40261 tgcgcggcgc gcagggcgag gttgctctcg tcggcgggca ctcccgcggc ggtccggccc
    40321 accacctcga tcccccagtc gcggtcggcg ctgacctcga tcgtgtcgaa gaggctgacc
    40381 gcctggtaga ccgtcgccag cgagtggaag ccgtcggggc ggggcggccc gacgcgtagc
    40441 tcgaggttga ctttcgcggg cacccgcacg catacccccg tatgcagctg cggcgccgac
    40501 gtcatgtccc ccaccctagt cagatcgcgg cgcgtgctgc agcgatggcg gcgtagccgg
    40561 cgatgtcgat ctgttcgccg cgcagggtgg ggtcgatccc cgcagcggtg agcacagagc
    40621 tggtcgcggc gggcgagccg gcccagccgg cgagggcgga gcggagcatc ttgcgccgct
    40681 gcgagaacgc ggcgtcgatg acggcaaaga cgtcctcgcg gcgcacgtcg gcgcggggcg
    40741 ggtcgcggcg gtcgatggcg accaggccgg aatcgacatt gggcacgggc cagaagatgc
    40801 tgcgggccac ggtcccggcc agccgcactt cgccatacca agccgccttg gcgctcgggg
    40861 caccgtaggt ccgcgagccg ggatcggcgg cgagccgctc ggccacctcg agctggacca
    40921 tcacgagcac ccgctcgatc gtcgggaagg tctcgagcag gtgcagcacg accggcaccg
    40981 agacgttgta aggcaggttg gcgacgagtt tggtcggggc gacgggcagg tcggtcaggc
    41041 gcagggcgtc ggcgggtatg acgcgcagtc gccccgcgcg ctcgggcgcg gcggcgctca
    41101 tggtcgccgg gagcgccgcg gcgaggcgcg ggtcgatctc gacggcgagc acctggtcgg
    41161 cggcctcgag caggccgacg gtgagggagc cgaggccggg gccgacctcg acgacgacgt
    41221 cgtcgggctg cacggcggcg gtcttgacga tgcgccgcac cgtgttggcg tcgacgacga
    41281 agttctgccc ccactgcttg gtcgggcgga cgccgtgctc ggcggccagg cgccggatgt
    41341 ccgccgcccc cagaaagtgc accgcctccg tcacgccgtt caccctatgg gtccgcgtga
    41401 acttttccgc ccagtgccac tttgtgtcgg tttgggcggg aaatattgcg aggcgaagcg
    41461 ctaggaactg gcactgggcg gaaaagttca cgcccccacc cggaacgaag atccgggcgg
    41521 gggcggggga tgttgcgagg cgtcaggccg cgtgcgcgca gccccagggg ctcaggccgg
    41581 cgatcgcgta gtagcggttg gcgatggtga tctgctgctc gcggctggcc aggtcggcgc
    41641 ggggggcgaa gtcgtcgccg cccatggcga gccagctggc cgtggcgaac tggaggccgc
    41701 cgtagtagcc gttgccggtg ttgatgtgcc agttgccgcc ggactcgcac atggcgatcc
    41761 ggtcccacat ggccgcgttg gccaggttga tgccggcgcc ggaggtggaa ccgccggtgg
    41821 acccgccgct cgaggagccg cccgaggacg agccggagct ggaggaaccg gaactcgacg
    41881 agccggaact ggacggcttc ggcgtggcgg ccggcttctc cttggtgccg acggcgatga
    41941 cctggttgcg gaccttcgtc gggatctcgg tcttgacgta ggaacgcttg acttccttac
    42001 cgtcgtgcag cacgaccgtg tagtagttga ccttctcgcc cttgcgaccg gccgtgacga
    42061 ccttggtttc accctggtag agcgaggagt cgttcttgcg gatcgtctcg aaggcgatcg
    42121 tgctggactt cgtgacggtc ttggtcttga cccgggtgac ctggagcttc ataccgtcct
    42181 ggatcggggt gtccagacgg ggcttgacga tgtccttgct gccgaccttg accttgagca
    42241 aggcgagcag gcccttgacg tcggtctcgg tggtggtctt cgtgacgctc ttgccgtcga
    42301 ccttcacggt gacctccttg ggggtcacca tggtcatcgc caggccggcg cggccgacgg
    42361 cggcggagcg ggaggcggat aggacggcgt cctcggcgcg caggccgagg tcggacaggg
    42421 cctcaccgac ggtggtcgcg gtggtccagt actccttctt ctcgccgtcg atggtgacgg
    42481 tgagcttgcg gccgtagcgg acgacgatct tctggccgtc gctgaccgag gtgccgacgc
    42541 ccggcacgac caggtcgtgg gggccgacgg tgatgccttc gtccttgagc acatcgccga
    42601 cggtgctggc gaaggcgtgg acattgctcg acgagccttc gacggtgagc gcaacggact
    42661 tgtcgaagtg cgcgacgccg gccactccgg cagccaggac gccgaccaca ccgagctggg
    42721 ccacgcgccg aaccggaaga aagttcactg aaactccaag tttgtaggtc cgatcccggc
    42781 gggtgaacgg cacagggcag ccgacatacc gcttcgaaga agacgtatcc cgcgctgctg
    42841 cgacgcctcg cggaccgggt ggggggaccc ccgggtcgtg ctgccgatga cggtcgtcgg
    42901 aatcggctga ccaaaccacc atgcccggat cccccacgaa aaatcaaatt ttgataacga
    42961 cggacgggac ccggggtagc cactcagatc acaaccgctg ccttgcgtca cacctgccac
    43021 acaggagcgc gccggcccgt gtggcatagg tcacgccacc gtcacgacgg ggtcaccacg
    43081 gcggccacgc agcgtcacca ggggccgtag aactcctccg cggtcgcccc cacggcctcg
    43141 cacaggtgcg ccacggcgat gcccagcacc gcggcggcgg agcgggcggt cagcgcgagc
    43201 agatagggcg agttgaccgc cccgcgccac gggtgcggcg tcagataggg cgcgtccgtc
    43261 tcgagcagca cccgctccag cggggtgacg gcgagcgcgt cgcgcagcgc gtggttgttc
    43321 ttgaacgtga tcgggccggc gaaggagaga tagaacccgc gctcgaggca cgcctgggcg
    43381 aaggcggcgt caccgctgaa gcagtgcatg acgacctgct cgggggcgcc ggcgtcgtcg
    43441 aggaccgcga ggatgtcgtc gtggctgtcc cggtcgtgga tctgcagcgg cttgccgagt
    43501 tccttggcca gcgcgatatg ccagcggaag gcctcgtgct gcacctcccg accctcgggc
    43561 ccggtgcggt agtagtcgag tccggtctcc ccgatcgtgc gcacgcgcgg gtcgcgcgcc
    43621 aactcgccga tccggtcgta tgccgcggcg agccccccgc tctgcgccag ccggggcgct
    43681 tcgttggggt gcagcgcgac gccggcgacg atcgccgggt gctcgtgcgc catccgcacg
    43741 ctgatctcgg cggcctcgac gtcgcacccg atctgcacca gtttcgttac ccccaccgca
    43801 gcggccgccg cgagggcgtc gtccaacgac agtgagggcg cgtcgtcgcg cacgatgtcg
    43861 aggtgagtgt ggttgtccac caacggtatc ggcagcggct ccgggcgcgc cgggatgctg
    43921 tgccgagagc tgtcgtgcgg cataccggct ctcagctcgt catccgggcg agctcgtcgg
    43981 cgatcaccga ttcgtcgagt ttgacgaaga ccggcgccgg cttgccgatg ggggtgccgg
    44041 actcaacggg acggcgcgcc cagggcggcg tcgccgcata gtcgccggtg atgatcggat
    44101 aggggctgcc gtcgtcgagg tcggcgacct cctcgacgcg gggcatcggc atgaagtcgc
    44161 cggtgccgcc gaggacggcg tcgacggcat tggccgagtg cggcagcatc ggcgccagca
    44221 gcgtgttgca gtccgaaacg cattgcgcga gaacatgaag caccgtgccg aggcgctcgc
    44281 gctggtcctc gcccttgagt ttgaagggct ccgtcttgga gatataggcg ttgacctcgc
    44341 cgacgacgcg catggcctcg gccgtggcct gccgcagccg ggagcggcgc agcagcccgc
    44401 cgacggtctc gaagccggcg gcgaccacgt cgagaacggc ctggtcctcc tcggcgagcg
    44461 gaccgcatac cggaatctcg ccgaagttct tgtggatcat cgtcgccgtg cggttgacca
    44521 gattgcccca tcccgcaacg agttcggagt tggtgcgctg gacgaactca cgccaggtga
    44581 agtccgcgtc ggaggtctcc gggccggccg cgcagatgaa atagcgcaat gcgtccggct
    44641 gatagcgcgt caggacatcg cgcacgagga tcgtatggcc gcggctggtg gagaactgct
    44701 ggctctccat cgtcagatat tcggaactga cgacctcggt gggcaggttg agttggccat
    44761 agatgccggg ctcaccgccg cgctccccct tgcccatgta gccgaggagt tcggcgggcc
    44821 agatctggga gtggaaggtg atgttgtcct tgcccatgaa gtagtaggac a
//
LOCUS       DAOPJG010000005        42879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_935_length_42879_cov_8.682468, whole genome shotgun sequence.
ACCESSION   DAOPJG010000005 DAOPJG010000000
VERSION     DAOPJG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 42879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42879
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_935_length_42879_cov_8.682468"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..2724)
                     /locus_tag="PKU75_01145"
     CDS             complement(268..2724)
                     /locus_tag="PKU75_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF79509.1"
                     /translation="MPAPLPARARVVIVGGGVIGTSIAYHLGHAGWSDVVVLERDRLT
                     SGTTWHAAGLMTTFGSTSETSTSLRRYSRDLYARLEAETGVATGFKPVGLIEAAADAD
                     RLEEYRRVAAFQRRFGLEIHEIGPAEMSELFPLAKTDDLLAGFYLPGDGRVNPVDLTM
                     SFARGARNLGVRIVEGVSVEKVLTTPQGRGSRVTGVQTSAGDIEAEVVVNCAGMWARE
                     LAAQNGVVVPNQAAEHYYLITEAIDGMSPDAPVFEDPAAYGYYREEGGGMMVGLFEPR
                     AAAWRVDGIPRDFSFGEIPPDWERMGPFVEKAMSRVPVAVEVGIRTFFCGPESFTPDL
                     LPAIGAAPGVGGYFVCAGMNSVGILSSGGWGRVMAHWITTGAPDVDVTAVNVDRFRGY
                     QLDERYRAERTAEILGTVYAAHTPGVQLASGRGIHRSAVHDRLVEAGGYLRDVSGWEG
                     AAWYAAPGQTPNAEPGWGHQPWFANWRAEHEAVRERAGLIDMSFMAKWRIRGPEAGPL
                     LDHLSAGAVDGAAETITYTQWLDDDARLQADLTVTKLTDGDFLLVASDTAYGHVEHLL
                     LAAAGDRDVAIEDVTTSWAQFAVQGPRSRDVLAGLVGADLGAEAMPFRSVRRTTLADK
                     EILLARITYAGELGYEVYVPAGDALAVWDTIVAAGQEHGLAFYGLNALASLRMEKGYR
                     DYGHDVDNTDGVYEAGLGFAVALDGGDFRGKAAAVTAKAAPATRRIVSVLLADPEPLL
                     FHAEPVLRDGEFVGYIRAASYGWTIGGAVGLAMVEAPEAVTKAWIDAGEWTVDVAGQL
                     VPARVSLRPFYDPTSARVRQ"
     gene            complement(2734..3771)
                     /locus_tag="PKU75_01150"
     CDS             complement(2734..3771)
                     /locus_tag="PKU75_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPF79510.1"
                     /translation="MTRTPFQLAALASAAVPGLDPVAAGAFPVDPGMAVDVALVEDTR
                     GRKWIVRASASPAAAVALDAGAAAAKLVAKHLDIRVPVTRGSVALKEGGRVHVHAAIP
                     GRGLRLAALDPAFPVVADLGRAIAAIHDLDPALFEDAGLESYSHDEYRQRRLAELDRA
                     AATGKVPAGLLTRWEAALENVALWQFVPVPVHGGLDGRHVLMALADDGTAKITGIVGW
                     RRAKVADPADDFAGLVRDAAAPAVIEVARAYARARHSRPDRHLLTRAKLVAELGYVTD
                     LFDALAAGYPQVVDAATARLQELAADVEGDELLPAPATPRDPAPAAAPTPDETATAPI
                     ETSERQDKTPG"
     gene            complement(3821..7141)
                     /locus_tag="PKU75_01155"
     CDS             complement(3821..7141)
                     /locus_tag="PKU75_01155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPF79511.1"
                     /translation="MTPSSTPPPAGPRISAAEIAAALRLPVPTEQQQEIIEHPPAPML
                     VVAGAGSGKTETMAARVVWLVANGYAEPEQVLGLTFTRKAAAELAGRVGTRLRALTAA
                     GVIDGAAGARPEETDGVVAAPPTISTYHAYAGRLVREHALRLGREPDARLLSEAAAWQ
                     YAHAVVSNDEGPLEEIGKAESTITNAVIDLAGEMAEHLVTPDQLDAWLAGFDERLEVI
                     EGAGRALLAESKAMRKALRAKRCLVPVLQRYAALKRDHGAMDFADQMAVAAELAMRFP
                     EVGRIERERFSSVLLDEFQDTSHAQLHLLAALFAPAGADGPGHCVTAVGDPHQSIYAW
                     RGASAATLAQFPAVFGAASVSVLPLSVTWRNDRAILDAANTVAEPLRAGAVVPVEPLS
                     AAPAAGDGRIEVARLETVDAEAALVADWIAQRRAEGAQSAAVLCRKRSQFPAVVDALE
                     AAGLPVEVVGLGGLLMTPEVQDVVALLTIAHDAGRGDRLMRLLTGPVCRLGAADLDAF
                     AAWARSRQHPPRRRGASSRGSDLSDAASDRVSLVEGLVDLAAASLTERAATGLSPAAV
                     RRLLDLAAALAGVRAVTGSGLVDIVAEAERALGLDIEILARPGYPVGVARAHLDAFTD
                     VAADFAAGGDRPTLGGFLAWLDAALAEERGLDRGVLDIVPGAVHVLTVHAAKGLEWDA
                     VAIPGLVESGFPAHGSATSKFDPDTGRWRSSEPGDGAWTIGLDGLPYDLRGDAASLPA
                     FAWRSAADAKELDDRRAAFRVASGAAGIAEERRLAYVALTRARRAMLLTCAVWGGPKT
                     PRVPSRFLLELREQRPDLLTTLGWCPDPPADATNPALAEPVSVSWPPHAPVADPVREG
                     AGAVAAAMGRPDLSDHVLPREVAVLVAERQARIDGSGSFPVQSAPGGPWRLLDALGGH
                     VSTSQLVSFAAEPDDFAVGVRRPMPQRPSVATRRGTAFHAWIERHYRRAALLEPDDLP
                     GSADPDTGEQVDLEDLIARFRASDWAEREPLDVEVMVETVVAGTAVRGRIDAVFLEPD
                     GSVTIVDWKSGAPPGGQEAHAAAVQLATYRIAYARLTGRDPDTVRAAFYYAATGETVY
                     PPLRSASELTALVATLRG"
     gene            complement(7138..10332)
                     /locus_tag="PKU75_01160"
     CDS             complement(7138..10332)
                     /locus_tag="PKU75_01160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPF79512.1"
                     /translation="MTATALRAAAAGTFAPDAQQQRALAHDGRLLRVLGGPGTGKTTL
                     AVELVADRVARGVAGPEQCLVLAPTRRAAAAVREQVTRRVAASTRTPLARTFASLAFG
                     ILRRAAIRDDLPTPFLLSGAEQDAILRELLAGHVAEPARAPDWPEHLGEAAGARGFRA
                     ELRDLLMRAVEHGLDAATLADLGRAHDRPEWTAAGAVLDEYDQVTALARPGGFDPAWL
                     LSGACDALESDPELLGELADQIRVVIVDDAQEVGAPGARLLGLLAGAGIAIVLIGDPD
                     AAVQTFRGADPRFLAEGWRDLGEGTTVVLGTAYRLPQEVAKADRRVTALIGALGGAHG
                     RRWVAGGPAGEVNVNVFRSAAQEATYIADAIRRAHLLDGVAWRDMAVLVRGQGRSDVV
                     RRVLAAAHVPVADAESDIPLGEEDAVRPLLQLLRAAAERVAVRADAAPEVLAADPDGG
                     WRLAPETAIDLLTSPYAGADAVTLRRVRRAVRRIAIDNGEGAPADELVARALARPGEL
                     SELGTEGAGARRIARMLDAATAALAAEAATPQDVLWAMWERSAVADTWRRAALAGGRV
                     GARADRDLDAVIAVLAAAADFADRRPGIGAAAFADHLAGQDVAADTIVTRGADRDRVS
                     ILTPHAAAGRSWEIVFVAGVQEGAWPDLRLRGSLLGSEDLLDALAGRERSIRAAQQVV
                     RHDEARLFHVALTRTRRRVDISAVRDGDDMPSPYLDVIAPPVVAPDGAVTERPLTEVA
                     TPLTLVGVVAALRRAVTAPADARAAPAERAAQAQQAATLARLAAVGVRGADPESWWAL
                     RALTDDRPRVPGSAFVPVSPSSLVTFASCRLRWLLTRAGGDGPAIGSAALGTLVHEVL
                     ADGGDADAAALGGRLDLSFGRLGLPAGWITRAKRAEAEAMLGRAAAYFEHAASLGWRR
                     LAAEQQFTATVGRAQISGIVDRLETDDDGRIRVIDYKTGSSKPAAADLARHPQLGAYQ
                     VAVAEGAFGPDAIGAGAVLVQLGKAATTKAATSVQAQTPLADDPEPGWAHDAVADAAH
                     AMAGSRFTATVGEGCRTCPVATSCPARPEGGMLT"
     gene            complement(10426..11595)
                     /gene="moeB"
                     /locus_tag="PKU75_01165"
     CDS             complement(10426..11595)
                     /gene="moeB"
                     /locus_tag="PKU75_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HPF79513.1"
                     /translation="MPFRTASYFGSAVTRPPDRPAPPELGPAERRRYARHLLLPEFGA
                     AGQQALAGSRALIIGAGGLGSVTATYLAAAGVGTIGIVDDDVVDESNLQRQVIHDTAA
                     IGRPKVDSAADRLTAINPLVRVERHPLRLTGANAVGLVSGYDVVVDGADNFATRYAVA
                     DACALTGRPHVWGSILRFDGQVSVWWPPHGPCYRCVFPAPPPAGSVASCAEAGVLGSL
                     CAAVGSIQATEAIKVLSGVGRPLTGRLLIHDALEGTWSTVPVARNPACRLCGPEATIT
                     APRDESAACETGGPRHTAEQLAQVLASDPGVVLVDVREPAEWAGGIIPGALLHPVGRI
                     EELAVDRDARVVLYCAAGGRSAAGAQRLRAAGFTDVTDLEGGISAWPGELADQPG"
     gene            11688..12332
                     /locus_tag="PKU75_01170"
     CDS             11688..12332
                     /locus_tag="PKU75_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF79514.1"
                     /translation="MAREFTAADTTRGQRLPRLERRAQLLEAAQSVFVDKGYHASGME
                     DIAERAGVSKPVLYQHFPGKLELYLALVDKHTAALVELVELVEAALNESDDHKDRTYA
                     SIRAFFDFVAREDEPFRIVFESDLNEVAAVRERIGWATEACVDAISRRIMLDTELPQP
                     VARLVAQGLVGMSQVSARSWLRDGTIPLDLAVEIIGRLAWRGVGGQRARLVAPA"
     gene            12462..12683
                     /locus_tag="PKU75_01175"
     CDS             12462..12683
                     /locus_tag="PKU75_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HPF79515.1"
                     /translation="MEVKIGIANVAREVSIEPEESASRVEELVRAAVTDGTPLRLEDA
                     KGRVVIVPAAAIGYVDIGESLKGRVGFGA"
     gene            12864..13136
                     /locus_tag="PKU75_01180"
     CDS             12864..13136
                     /locus_tag="PKU75_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPF79516.1"
                     /translation="MIGTIIGAIIAGAIIGGLARLILPGRQGISLPVTIILGILGSLI
                     ASWLVYNLGYDNENGGFQFIPFIAGVLVAAGLIVGYGAMTGNKQVR"
     gene            13279..13953
                     /locus_tag="PKU75_01185"
     CDS             13279..13953
                     /locus_tag="PKU75_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HPF79517.1"
                     /translation="MSDVSCPDSADRTATVDLLGMLALGELTAFSRLAADADMAPAVA
                     GREAFARLALVEFGHYELLLARLRDLTGAPEAAMAPYAPVFAAYHERTRPGTWLEAVV
                     KAYVGDGIAADFSREIARFVDAESRAAIEAAMDDRERSAVLVRLAAEGMQSDPHVRGR
                     LALWARRLHGEAVSQAQTVAAQRESLTTLLTGSGPQLDLVALGELFARLARRHTERMA
                     ALGLEA"
     gene            14156..15643
                     /locus_tag="PKU75_01190"
     CDS             14156..15643
                     /locus_tag="PKU75_01190"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPF79518.1"
                     /translation="MSDKTFADFGVHPDIVAALAEHGIVTPFPIQAMTLPVALGGHDI
                     IGQAKTGTGKTLGFGIPMLQRVVAPGDEGYAALPAPGAPQALAVAPTRELAVQVAKDL
                     ERAGAQRGVRVLTVYGGRAYEPQVEALRKGVEVVVGTPGRLLDLAGQGHLTLAHARIV
                     VLDEADEMLDLGFLPDVEKLLALTPAGRQTMLFSATMPGAVVALARRYMTQPTHIRAM
                     SEEGENQHTVKAIEQFVYRAHALDKTEMLARILQARGRGLTMIFSRTKRTAAKVADEL
                     TERGFAAASIHGDLGQGAREQALRAFRSGKVDVLVATDVAARGIDVDNVTHVVNFQCP
                     EDEKTYVHRVGRTGRAGNTGVAVTFVDWDDIPRWGLINKTLDLGIPEPQETYSSSEHL
                     YTDLDIPREAKGKLPKSAQTRAGLAAEEIEDIEGPSKKAGGRGGSRSGRGGRTGTRDG
                     AREGRSGQARAARDGAAPEGQAERTPRSRSRRRTRGGKPAAATTD"
     gene            complement(15773..16825)
                     /locus_tag="PKU75_01195"
     CDS             complement(15773..16825)
                     /locus_tag="PKU75_01195"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPF79519.1"
                     /translation="MTTQVPAILKDSADAPFRQGLVERRDLRPDDVRIDIAYAGICHS
                     DIHTVREEWGPCIFPIVTGHEIAGVVSEIGSEVTKFKVGDHVGVGCLVDSCGECDYCV
                     KGSENFCEKGAVGTYNSKNYDGEIAQGGYSQQIIVTERFVLRIPDAISLEVAAPLLCA
                     GITLYTPLKRWGAGPGKKVAIVGMGGLGHMGVKIAAAMGADVTVISQTMSKEEDGRAF
                     GAVDYRATEDGSVFKELRNSFDLIVCTVSADLPLDKYLGMVKPFGALVLVGLPEKPQN
                     FQVFSLIMGDKVLTGSNIGGIPITQEMLDFCAEHDIAATVETISADEVDAAYDRVVAS
                     KVRYRFVIDTSTITPE"
     gene            complement(16845..17627)
                     /locus_tag="PKU75_01200"
     CDS             complement(16845..17627)
                     /locus_tag="PKU75_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPF79520.1"
                     /translation="MPPRSRAKGATILAVANQKGGVAKTTSVASLGAAFAELGKRVLL
                     VDLDPQACLTFSLGIDPDQVEASVHDALTGAVPLADVIFECGDGVDLIPSSIELAGTE
                     SVLLGRPGREYILQELLAPVATAYDLILLDCSPSLGVLTLNALTAARGLIVPMPCEML
                     GHRGVGQLLDTVRDVKRILNKKLDVVGVLPTMYDGRSTHQQDVLADVGERYGIPVLAP
                     PIPRTVRFAEAPAVGRSILETSRTSKGAQAYRAVAKDILTRL"
     gene            complement(17736..18641)
                     /locus_tag="PKU75_01205"
     CDS             complement(17736..18641)
                     /locus_tag="PKU75_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPF79521.1"
                     /translation="METETLRWFQHVADGMTVTEVAETYAVSQPGVSRALARLEEELG
                     APLLRRSGRVLRLTAAGAVFKAHLDAMMAQYDDAVAAVEQLLDPGTGIVTLAYPLSLG
                     TWFVPGLIRDFRAAHPGVRVRVARSSVGAVGAISRLLATREVDLEITTGRVSGPGVEW
                     RQVAIEPLLLAVPLDHRLAGAEETELAHVAREPFIIRRAPSEMRAQVLQLCAAAGFEP
                     DIAFEVDDLPTVRGFVAAGLGVGVVPALGRSVPTELGPVRLIPLRDNDARREVGIAWL
                     SERRLLPAADLMRRFALAEAGRRGA"
     gene            18720..18962
                     /locus_tag="PKU75_01210"
     CDS             18720..18962
                     /locus_tag="PKU75_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79522.1"
                     /translation="MSIKNIRISLRHHRAAVSARQDMLRQLSVYTTPAEIEDMLAAVD
                     GQDSPDADLMREVLGDKLARAYRDSARPAFGMHVAA"
     gene            19050..21107
                     /gene="pta"
                     /locus_tag="PKU75_01215"
     CDS             19050..21107
                     /gene="pta"
                     /locus_tag="PKU75_01215"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPF79523.1"
                     /translation="MSTRVYIASGEAHSGKSAIAMGLVSALARHAGRVGIFRPVVTDP
                     EADPVLTLLASRLERPIDLRAASGVMLQDLHDDRDAAMAEVVARFHAAAADTDMMVIV
                     GSDYSGVPAPTEFAANASLAAHLDAPMILVVNGAGRTPEQIALAAELMAEGAHARHAQ
                     VLAVIVNRVDGPAEAIVAATQQATRGIPTYTVPNVPILDAPTVRELMGAVNGELLSGD
                     PARLDNESLGFVVAAMTMPHVLDRLLPAGAVITPGDREDVLLGVLLADRGKTFPSPSA
                     VFLNGGFTPSPQVQQLISGIDSSLPVISCAGGTLATTAALTGVKGSLNPASVRKIAAA
                     EKVLAESIDTDALLERLGAASHGAVTPLMFEQNLLTQARKAGRHIVLPEGDDDRILEA
                     AALILERQAARLTILGDPAQIAARAHALDLDLTGARLLSTTQEPNLERYAQEYARLRA
                     HKGVSIDIARDRVIDGAYFGTMMVLLGEADGMVSGANHTTAHTIRPALEVVKTVPGVS
                     VVSSVFFMCLADRVLVYGDCAVNPDPTAEQLADIAVSSAATARQFGIEPRVAMLSYST
                     GGSGSGLDVEKVRTATDLVRQRDPELAVEGPIQYDAAVDPDVAATKLSGSKVAGQATV
                     FIFPDLNTGNNTYKAVQRSAHAVAVGPVLQGLNRPVNDLSRGALVRDIVNTIAITAIQ
                     AGA"
     gene            21111..22310
                     /locus_tag="PKU75_01220"
     CDS             21111..22310
                     /locus_tag="PKU75_01220"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPF79524.1"
                     /translation="MTSPDGRVLVVNAGSSSVKYQLLQSDSGEVLAKGLIEQIGESSG
                     GKHRHTVAGTDNTRTGEIGDMTAALASMAAAFADFGPDLDAEPPMAVGHRVVHGGTRF
                     SATTRITAATTDAIRDLIPLAPLHNPANLAGIEAALAQFPDVPQFAVFDTAFHSTLPA
                     AAYTYAVPKVWREDYHVRRYGFHGTSHHYVSERVSALMGRADLATIVLHLGNGCSACA
                     VLDGESVETSMGMTPLEGLVMGTRCGDIDPAVPFHLARTEGLDVDQLDTALNKQSGLL
                     GLTGDNDFRTIAERAAAGDREAQAAIDVVVHRLVKYVGAYAAVLGRLDAIAFAGGIGE
                     HNAALRAAVLGRLGILGVEVDAAANEGADGEALLTTPASRVAAFVIPTNEELQIAREC
                     LEHLESP"
     gene            22307..23734
                     /locus_tag="PKU75_01225"
     CDS             22307..23734
                     /locus_tag="PKU75_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPF79525.1"
                     /translation="MSRNTWSGQRLPHPPGRRRFVGDIEREADPNTPVQNLMRRVHGL
                     PELVEFKAFREKFVFALSARVCAQVADDVHFDKALAPAVLALRRFSGDALFTAFTHEP
                     AWGVAHDLLVPAFTKPAMRGYHDTMNAVADDLVARWAASTTPVDVADDLTRMTLETIG
                     RTAFGHSFGTFAHQERDPYVAAMVTLLSDSGQAAGLSTLPLGQLITRRPSRRARAAYA
                     TQVEITEDIIEARLAGGGQHGGGQQRGGADLLGRMLFPAHAESGARLDRRNVINQVLT
                     FLTAGHETTSGALSFALWYLANDDRVRAAAQAETDDLLGPDPQSTPTFEMVPKFRYVR
                     RVLDEALRLWPTAPAIGRSPRAVTTVAGWTMTPQDWLLVFIPAVHRDPAVWGPDPDRF
                     DPDNFLPERVRARPAYSYLPFGVGTRACIGRQFALHEAVLVLARLLHRFDFAADPAYD
                     LRVTERLTLMPKGFRLTVRERSLVA"
     gene            complement(23813..24703)
                     /gene="purU"
                     /locus_tag="PKU75_01230"
     CDS             complement(23813..24703)
                     /gene="purU"
                     /locus_tag="PKU75_01230"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HPF79526.1"
                     /translation="MGVPVWGYYPAVPTRFVLTLSCPDRPGIVAAVASTLANLGANIL
                     ESQQFDDPRTGRFFMRVLVEPVAGETIEQADLHGAVAAVGKRFDMDFQLRRADAPYRT
                     ILMVSKFGHCLNDLLFRWRNGGINMDVAAVVSNHPDLEPLVASYGVAYYHIPVTPDTK
                     AEAESRLLSLVDELDVDLVVLARYMQILSDELTARMSGRIINIHHSFLPSFKGAKPYH
                     QAYERGVKLVGATAHYVTADLDEGPIIEQDAIRVDHRMTPEDLVRAGEECEERVLAHA
                     LRWHCESRVLLAGDRTVVFS"
     gene            24768..26024
                     /locus_tag="PKU75_01235"
     CDS             24768..26024
                     /locus_tag="PKU75_01235"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPF79527.1"
                     /translation="MDSREIAVAAFAVPVLLAVLGLWWFRRRRRDEIFEGVTPGELPA
                     LGQQVTRRRVPRGRQEYAGPVAVRFSPPDGLTPGLVGTVIDGQAHVIDVTATIIDLAV
                     RGHLRLVAEAADPDTPPPPPRGPAPKTPVRQSDHDWRLIRLPAPDGDRLLPFEHKLLD
                     AVFRYGPEVTVRELKARGFDLTMREAQIGLYREVVDRGWYTKHPRDRNRRLGCLGWLL
                     VVAAVLAALAGITARPWEPARTPMLALGAGLLFAAILLIWGGRGRTPRTAEGTAARIQ
                     ALGFREYLTKAEANQIRFEEARDLFSRYLPYAMVFGVAERWARTFADVAASARLQGVG
                     DAFFDLTWLGGFDVLGDTLGAGLQIADAVGSVADFSAGIDIPDLDLADGLGGIASSVG
                     DFADSAGGLLDFGDGCSGCDGCDFGI"
     gene            complement(26050..26391)
                     /locus_tag="PKU75_01240"
     CDS             complement(26050..26391)
                     /locus_tag="PKU75_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79528.1"
                     /translation="MAAAVVALEALALVGLAAGYAVGIAEETESSLSRTLASIGLFLV
                     GATILASMARAWLRGQEWQRMATLVLNALLVPVAFSVIRGNGALVGVPVLLLAVTGVT
                     AALLAGSSTPD"
     gene            26647..26895
                     /locus_tag="PKU75_01245"
     CDS             26647..26895
                     /locus_tag="PKU75_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPF79529.1"
                     /translation="MDWRDRAACLDEDPELFFPIGNTGPAILQIEEAKAVCRRCEVID
                     TCLKWAIESGQDSGVWGGLSEDERRALKRRNARARRAG"
     gene            complement(26944..28431)
                     /locus_tag="PKU75_01250"
     CDS             complement(26944..28431)
                     /locus_tag="PKU75_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPF79530.1"
                     /translation="MASVLAEQLNRETTLTESQIERIHVLLGEWQLLSDLSFADLVLW
                     VRTRAGDWRAVAHVRPNTGQMVFFEDIVGQHAVGDRSRLLERALDEGAILRRGGPILD
                     NDHAIREEAVPLRDGDQTIALLTRHTNLTALRTPSRLELSYQGLAAALVRMIAAGEWP
                     GASAPTGSRRGAPRVSDGVIHLGVDGIVLYASPNAMSAIHRLGHPGEVIGEQLATVFA
                     DVLRDQALVDEALAMVTMGRAAWRADATTRSGSVALRAIPLTEGGERVGAIVLLRDVT
                     DLRRREHELLSKEATVREIHHRVKNNLQTVAALLRLQARRLPDGAGRSALDEAVRRVG
                     TIALVHETLSHGFDETVDFDEIARRGLHAIVEVATTDVPIGSRVEGSFGRLRAEDAMS
                     LALVISELTQNAVEHGLRETGGTVTLWVERVEEDGGDGDVLQVAIEDDGTGIPAGFRP
                     GKAGLGTQIVNSLVQGLRGAIRWEDAQPHGTRVRFFARLRPLDPA"
     gene            complement(28512..33389)
                     /locus_tag="PKU75_01255"
     CDS             complement(28512..33389)
                     /locus_tag="PKU75_01255"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HPF79531.1"
                     /translation="MTATSEEIQQRSDRIAQACELASEEQGPPGEGELIEAYFRHAPT
                     EDLLERSPQDLLDIVRSHRELGTQRPAGTAKVACTTPPLGDGPSHTKIQIVTDDMPFL
                     VDSVRAQLLSMGRGIHTVVHPILRVERDATGALVRILPDDAVPSEGQSQSVGVLNESW
                     MHLEIDRESDAPDRDEIVARLRGVLSDVREAVEDWPKMRARARMIAAELPAVVPADIP
                     EAEVKQAVELLDWLADNHFTFLACRDYRLAPTEGGTGLIPVSGSGLGLLRADPPMGVP
                     RPALSDAVLAKATERRVLFVTKANSRSSVHRPVHLDYVGVKTFGPDGQITGERRILGL
                     FTASAYAESVGRVPYLREKVAAVMRKAGFQDESHSSKDLLGILEDYPRDELFQADVDQ
                     LVDVGTSVLTLAQKRRARVFLRPDDYGRFVSALVYIPRDRYTTAVRLRVEGILREALH
                     AKSIDYTARVSESPLARVHYVARLEPGAEMPDIDIDLLNDKIGAAIRTWDEDFRDAAI
                     REVGEEAAGPLVAAYGPAFSISYKDDFEVAQGLADLRRLELLEQPTDTEFALYKPRSG
                     DEAIRRFKLFRREPISLTRVLPLFTHMGVEVVDERPYELTRADGTPMYVYDFGLRAPA
                     DDLWDSMSHAELRELFEGAIDAVWNNRAESDPLGALVLRAKLTWRQVVILRAITKYVR
                     QTQSRFSENYIVSALVHNKDIAADLVALFEARFDPDRAHEGREEAEEEIAARIQAALD
                     DVSSLDEDRILRSCLGVIRAALRTNHFQLGPDGEGKDYFSIKLDPTKVPDLPQPRPAF
                     EIWVYSPRVEGVHLRFGPVARGGLRWSDRREDFRTEILGLVKAQMVKNAVIVPTGSKG
                     GFYAKQLPDPAVDRGAWLEEGKAAYRVFLSGLLDLSDNRVGTDIVPPERVVRHDGDDP
                     YLVVAADKGTATFSDIANGVAQSYGFWLDDAFASGGSAGYDHKAMGITARGAWESVKR
                     HFREMGIDTQSEDFTVVGVGDMSGDVFGNGMLLSEHIRLVAAFDHRHIFLDPAPDAAA
                     SYAERRRMFELPGSSWDDYDKGLLSEGGGVYPRSAKSVPVTPQVAEVLGIDSGVTSMT
                     PAELIHAALLAPVDLLWNGGIGTYVKASDETHGEIGDRANDAIRVDGNQLRCHVVGEG
                     GNLGLSQRGRIEAARSGVRLNTDAIDNSAGVATSDHEVNIKILLGQVMRDGALDLPAR
                     DELLRSMTDEVAADVLRDNYEQNVLLGNARAQQRIMLPVHMRFIRYLEEHGGLNRELE
                     FLPSETELGDRLAAGTGLVSPEFCVLVAYSKLTLKRDLLASGFPDDPWLESELRGYFP
                     PALPERFPEAVAAHPLRRDIIVTGVANDIVNRGGITFAYRATEETGASGEQIARAYVI
                     AKEIFGVADYLDRVEALDNVVSTQQQTELYLEVRRLLDRAVRWFITARPSTLDVGSEI
                     ERYQPIIGAWRPRLGELLVGSERERLEEATAARVAEGVPEDLARDCASLLDAFSLLDI
                     ADICIDKDLDPAVVAPLYYLNSERLGVDKLLGKITQLQREDHWEALARGALRDDLYGI
                     LDAITRAVLDGAPAGEQDPDKLLDGWLTAHSETVQRAQRSLAGIDRLDQPTIAALSVA
                     LRTLRSVVRAGASEG"
     gene            complement(33501..34013)
                     /locus_tag="PKU75_01260"
     CDS             complement(33501..34013)
                     /locus_tag="PKU75_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79532.1"
                     /translation="MGYAVNGAGDPASLAATATALQRVARDLVDAADSLDSRPAPGAP
                     RRSPANRRLLAELADVCRAAAAVTRTGGDELNRAAYSQARLGHQSAALADEAAAAGLV
                     TGPTGYAMRPGIVGVADPADSATRSAHLAGVRDRAAQLEQHMVREREQLIARLSAAAA
                     DAEALAGGLR"
     gene            complement(33998..34261)
                     /locus_tag="PKU75_01265"
     CDS             complement(33998..34261)
                     /locus_tag="PKU75_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79533.1"
                     /translation="MPAPPDPALTLGLLAERCDATVESLLLALPSTGDRLAQAAVDGY
                     LDAVMDLLRMLAAAAREGAGRLPTAPVSQAAPHVAAAPPWGMP"
     gene            complement(34327..34824)
                     /locus_tag="PKU75_01270"
     CDS             complement(34327..34824)
                     /locus_tag="PKU75_01270"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HPF79534.1"
                     /translation="MRITESADLAGTPDEVFALRATEAFQDEKCLRTSPREHTVAVDE
                     QGERTILRTTRAMPTHNLPDAARSMVGDELIVDEVQDWGPPAADGSRRAQVTITVHGA
                     PISLAGTLEVYPTADGSHQALEADLKAKVPFIGGKIEKAAAPAISHGFDLEAEIINDW
                     LAAER"
     gene            complement(34885..36513)
                     /gene="pruA"
                     /locus_tag="PKU75_01275"
     CDS             complement(34885..36513)
                     /gene="pruA"
                     /locus_tag="PKU75_01275"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPF79535.1"
                     /translation="MDAVTTVPIPVNEPVRDYAPGSSEREALLTELARIGGETRDLPH
                     VIDGAEVAGSGPRIPVRQPHAHAKIVGRMRNGGAKEAGAAIAAARAAAPGWRELSYDD
                     RAAIFLKAADLLAGPWRATLNAATMLGQSKTAYQAEIDSACELIDFWRFNVHFGRQIL
                     QEQPMVNAPGVWNRLDHRPLEGFVYAITPFNFTAIAGNLPTAPALMGNTVLWKPSPTQ
                     QLAASYTMELLQAAGLPPGVINMVTGDGIAVSTVALTDPGLAGIHFTGSTATFQHLWQ
                     QVGANLSGYHTYPRLVGETGGKDFILAHPSADPDVLRTAMIRGAFEYQGQKCSAASRA
                     YVPRSLWRVIKDDLVELTNGLSQGDVGDLRNFMGAVIDERSFARNAAAIDRAKATAGI
                     TVHAGGTYDDTDGYFVRPTVLVADDPVDEIFTTEYFGPILAVHVYPDSRFRSVMAQME
                     SVAPYALTGSIIAQDRRVIAEASAALRFAAGNFYINDKPTGAVVGQQPFGGSRASGTN
                     DKAGSMINLMRWTSPRAIKETFVPPTAHGYPHMA"
     gene            complement(36607..37011)
                     /locus_tag="PKU75_01280"
     CDS             complement(36607..37011)
                     /locus_tag="PKU75_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79536.1"
                     /translation="MALRRLYIPVTAADVTALIASGELPGPVRTAYGVSPALVAATPR
                     ADEEEREYAAFLDAVAAGAAAGDRVAVAALDLPDAEVDWPDTGHTVEVHRAVLRREIV
                     AFHVGEEPGEDADLLWFDITEAGLVAQHLTDS"
     gene            complement(37013..38413)
                     /locus_tag="PKU75_01285"
     CDS             complement(37013..38413)
                     /locus_tag="PKU75_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPF79537.1"
                     /translation="MIDRLSPADSSFLYLEEAHTPTHVGQVLIFDAPADYDTHALSMA
                     ISARVERMPRYRQRVHSLPGRVVSPVWVDDTEFDLDYHVRRSALPAPGDRAQLQDFVA
                     RVCARPLDRHRPLWEVYVVEGLDDGRIAVISKVHQALIESGDITGLSSEARAQDAPGA
                     GWTPTPGPSARELLSDTAISLVKRPGSVVGTVRGGLAELRATAGRVGRVAGTVAKAAA
                     RPASGRTFAVESAGARRYLMVDGSLADLREVRKAMSTGGHKRPSLHAVALTVVTGGLR
                     IWLQSRGEHVPAQRSVRAMVPVSVARDGTPYGSTVEALFIDLPVGESSPAMRLRQIQF
                     ALDHQLAQHSGVRAADLMGMAGFAPPALHSLGVRVGASLSTRLFDLVITNVPGPQRQM
                     YADGLPLVASYPVLPLTKGHAMAIGMTSYLGAMHYGVLADREAIPDLDQLRAAIAEAV
                     EELLHWVADPFGTEGN"
     gene            38485..38580
                     /locus_tag="PKU75_01290"
     tRNA            38485..38580
                     /locus_tag="PKU75_01290"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38520..38522,aa:Sec,seq:tca)
     gene            38606..39967
                     /gene="selA"
                     /locus_tag="PKU75_01295"
     CDS             38606..39967
                     /gene="selA"
                     /locus_tag="PKU75_01295"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPF79538.1"
                     /translation="MTGQRHGDPRRGIPRTDTLLADPQIASYAAALGAPRVRAVIDAV
                     TAAARAGAVAPDAVAAEVRARLARWAASATTLVPVLNATGVVVHTNLGRAPLTPAARE
                     ALVAAAGYVDVEFSLESGLRAGRGRGTLAALRERVPGAQAALVVNNGAAALSLAVTTC
                     AQGRELIVSRGELVEIGDGFRLPDLIRSTGVVIREVGTTNRTHPGDYEEAIGAQTGAV
                     LKVHPSNFVVRGFTAEVSVSAASRIARAHGVPLIVDTGSGLLEPDPLLPDEPDAATAL
                     AEGADLVVCSGDKMLGGPQAGIVLGRADLVEAMRRHPLARAFRADKLTHAALEATLRG
                     PRGLVSDYLHAPTEELRGRTEALAQRLRAARPDLMTDSRVVAVAGAVGGGGGAEVPLP
                     GWALALPEALALVLRAGRPAVVARQEGGRTLVDLRCIPVADEADLAEALLAGLERLAA
                     EGN"
     gene            39967..41799
                     /gene="selB"
                     /locus_tag="PKU75_01300"
     CDS             39967..41799
                     /gene="selB"
                     /locus_tag="PKU75_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPF79539.1"
                     /translation="MHVIATAGHVDHGKSTLVRALTGIEPDRWEAERRRGLTIDLGYA
                     WTTLPGAGEVAFVDVPGHRRFIGNMLTGLGPAPAVLVVVAADAGWSAQSEEHLRAIDA
                     LGLTHGLLVVTRADLADPAMAARVGAAARERISASSLGEVDSVIVSAVSGQGLDDVRD
                     ALGRLCATLPAPVRDGRVRLWVDRAFTIPGSGTVVTGTLGAGTIAVGDVLEVAGRSET
                     RRRVTVRGLQSLERSREAVTAVARVAVNLRGVAAGEVHRGDALLTPEGWRSTANVDVR
                     TAGPVADLPREVLCHTGTAAVPVRVRPLSAGAARLSFERPLPLANGDRLILRDPGTEG
                     ALTGAVVVDTAPAALTRRGDAARRGAEVEASDQVTLADEVRRRGHLSRADATLLLGHD
                     PGPPSREHFSPVESARRGHRVNDVVGVGDYFATRERVAAWTRAVLAVVDRRAASDPLD
                     PLVPMAAARDAAGVPDLVVLEQLCDAAGLQVERGRVCRPGTQPSLGPAQAGLDHLLAR
                     LTEDPFAAPEAPDLAAAGLGPRELAAAEALGRLVRLPDDIVLLPTGPAQAMRILAALA
                     QPFTTSQARQALGTTRRVAIPLLEHLDQRGWTRRLDAGHREVVR"
     gene            41840..42832
                     /gene="selD"
                     /locus_tag="PKU75_01305"
     CDS             41840..42832
                     /gene="selD"
                     /locus_tag="PKU75_01305"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPF79540.1"
                     /translation="MTETRLTQFAAGGGCACKIPAAQLEGIVAGLSGATHPDLVVGLD
                     TGDDAAVVRIAGGQAVISTADFFTPVVDSAYDWGRIAAANALSDVYAMGGKPVLAINL
                     LGWPVDVIDTGLIPDVLRGGADIAEGAGIPLAGGHSITAPEPIYGLAVTGVADPDRLL
                     RNDIAEPGLPISLTKPLGLGILGNRHKATGEIFPEAIATMTQLNDAASAAALAAGVRA
                     ATDVTGFGLLGHLFKMCRASGVSARIDRAAVPVLEAAVASLREGYVPGGSRRNLDWVR
                     PHLDAAPGVSEDDLLLLADAQTSGGLLIVGEIPGAPVIGETTRHTGSGPRITIP"
BASE COUNT         5992 a        15201 c        15595 g         6091 t
ORIGIN      
        1 gctcggggcc ctgctcgcca tccccatcgc ggccggtctg ctgctgctct atgaagaggt
       61 gctcgtccct cggcagcgca acgtctgacc tgccgccctc gtccgccgac ccgcctatgg
      121 agtgtgatct agatcacata ggcggtacgg caaccttttg gcccgctgtg acgtctgtca
      181 gggtgtcggc gcgctggggg gcgagccggc ccggcggtgg ggtgagctcg tggggacgcg
      241 agcccccgcc gccctctctt tttccgccta ctgccgaacc cgggcgctgg tggggtcgta
      301 gaacggtcgc agcgagacgc gggcgggcac gagctgcccg gcgacgtcga ccgtccactc
      361 cccggcgtcg atccaggcct tcgtcaccgc ctcgggcgcc tccaccatgg ccagcccgac
      421 ggcgccgccg atggtccagc catatgacgc cgcccggatg tagccgacga actcgccgtc
      481 gcgcagcacc ggctcggcat ggaagagcag cggctccggg tcggcgagga ggaccgagac
      541 gatccggcgc gttgccgggg ccgccttcgc tgtgaccgcg gccgccttgc cgcggaagtc
      601 gccgccgtcg agtgcgaccg cgaaacccag ccccgcctca tacaccccgt cggtgttgtc
      661 gacgtcgtgt ccgtagtcgc ggtagccctt ctccatccgc aggctggcca gggcgttgag
      721 gccgtagaac gccagcccgt gctcctggcc cgcggcgacg atggtgtccc acacggcgag
      781 ggcgtcgccg gccggcacat agacctcgta gccgagctcg ccggcgtagg tgatgcgggc
      841 gagcaggatc tccttgtccg ccaaggtggt tcgtcgcacc gaccggaagg gcatcgcctc
      901 ggcgccgagg tcggcaccga ccaggccggc gagcacgtcc cgggaccggg ggccctggac
      961 ggcgaactgg gcccaggacg tcgtgacgtc ctcgatcgcc acgtcccggt cgcccgcggc
     1021 ggccagcaag aggtgttcga cgtgcccgta tgccgtgtcg gaggcgacga ggaggaagtc
     1081 accgtccgtc agcttggtca ccgtcaggtc ggcctggagc cgggcgtcgt cgtcgagcca
     1141 ctgggtgtag gtgatcgtct cggccgcacc gtcgacggcc cccgcgctga ggtggtccag
     1201 taggggtccg gcctcggggc cgcggatgcg ccacttcgcc atgaaggaca tgtcgatcag
     1261 gccggcccgc tcgcgcaccg cctcgtgctc ggcgcgccag ttggcgaacc acggctggtg
     1321 accccagccg ggctcggcgt tcggggtctg ccccggcgcg gcataccacg cggcgccctc
     1381 ccagccactg acatcgcgca ggtacccgcc cgcctcgacc aggcggtcgt gcaccgccga
     1441 gcggtggatc ccgcggccgg aggcgagttg cacgccgggg gtgtgcgcgg catacaccgt
     1501 gccgaggatc tcggcggtcc gctcggcgcg gtagcgctcg tcgagctgat aaccccggaa
     1561 gcggtccacg ttgacagcgg tgacgtcgac gtccggggca cccgtggtga tccagtgcgc
     1621 catgacccgg ccccagccgc cggaggagag gatgccgacg gaattcattc cggcacagac
     1681 gaaatagccg ccgacgccgg gtgccgcgcc gatggccggc agcagatcgg gggtgaagga
     1741 ttcgggcccg cagaagaagg tgcggatgcc cacctccacg gcgaccggaa cccgggacat
     1801 ggccttctcc acgaacgggc ccatgcgctc ccagtcgggc gggatctcgc cgaagctgaa
     1861 atcgcgcggt atgccgtcca cccgccacgc cgccgcgcgc ggctcgaaga ggccgaccat
     1921 catgccgccg ccctcttcgc ggtaatagcc gtatgccgcg gggtcctcga agacgggggc
     1981 gtccgggctc atcccgtcga tggcttcggt gatgaggtag tagtgctcgg cggcctggtt
     2041 ggggacgacg acgccgttct gggcggcgag ttcgcgggcc cacatgccgg cgcagttgac
     2101 gacgacctcg gcctcgatgt cgccggcgct ggtctggacg ccggtgaccc gcgaaccgcg
     2161 tccttgtggg gtcgtcagca ccttctcgac cgacacgccc tcaacgatgc gcactccgag
     2221 gttgcgggcg ccgcgggcga aggacatcgt caggtcgacc gggttgaccc ggccgtcgcc
     2281 gggcagatag aagccggcga gcaggtcgtc ggtcttggcc agcgggaaca gctcgctcat
     2341 ctcggccggg ccgatctcgt ggatctccag gccgaaccgg cgctggaagg ccgccacccg
     2401 gcggtattcc tcgagccggt cggcgtcggc ggccgcctcg atcagtccca cgggcttgaa
     2461 gccggtggcg acacccgtct cggcctcgag ccgggcgtac aggtcgcggc tgtagcggcg
     2521 caggctcgtg gacgtctcac tcgtcgaccc gaaggtcgtc atcaatccgg cggcatgcca
     2581 ggtggtgccg ctggtgagcc gatcccgttc gaggacaacg acatcgctcc agccggcgtg
     2641 gccgaggtga taggcgatgg aggtgccgat caccccgcct ccgacgatga cgacacgggc
     2701 acgggcaggg agcggcgctg gcaccctccg agcctagccg ggcgtcttgt cctgccgttc
     2761 cgaggtctcg atgggggcgg tggcggtttc gtcgggagtc ggtgccgcgg ctggtgccgg
     2821 gtccctgggc gttgccgggg cggggaggag ttcgtcgccc tcgacgtcgg ccgcgagttc
     2881 ctgaagccgg gccgtggcgg catcgacgac ctggggatag ccggcggcca gggcgtcgaa
     2941 gaggtcggtg acatagccga gttcggcgac cagcttggcc ctggtgagca ggtggcggtc
     3001 gggacgggag tggcgggcgc gggcgtaggc ccgggcgacc tcgatcacgg cgggagcggc
     3061 ggcgtcgcgg accaggccgg cgaagtcgtc cgccggatcg gcgaccttgg cccggcgcca
     3121 gccgacgatc ccggtgatct tggccgtgcc gtcgtccgcg agcgccatca gcacatgccg
     3181 gccgtccagc ccgccgtgca ccggcaccgg cacgaactgc cagagggcga cattctccag
     3241 cgcggcctcc caccgggtga gcaggccggc cggcaccttg ccggtggcgg cggcgcggtc
     3301 gagttcggcg agccggcgct gccggtactc gtcatggctg taactctcca gtcccgcgtc
     3361 ctcgaagagg gcggggtcga ggtcgtggat ggcggcgatg gcgcgaccga ggtcggcgac
     3421 gacgggaaac gcggggtcga gcgcggcgag ccgcagtccg cggccgggga tggcggcgtg
     3481 cacgtgcacg cgcccgccct ccttgagtgc gacactcccc cgcgtcaccg gcacccggat
     3541 gtccaggtgt ttggcgacga gcttggcggc ggcggcgccg gcgtcgagtg ccacggcggc
     3601 agccggggaa gcactggccc gcacgatcca cttgcggccg cgggtgtcct cgacgagggc
     3661 gacgtcgacg gccatcccgg ggtcaaccgg gaaggcgccg gccgccacgg gatcaaggcc
     3721 gggcacggcg gcgctggcca gagcggcaag ctggaagggc gttcgcgtca cgatcccacc
     3781 gtatgccgtc cgcgcccgcg catccccgcg ccggccggac tcaaccccgg agtgtcgcga
     3841 ccagagcggt cagttcgctc gcgctgcgca gtggcgggta gacggtctca ccggtcgcgg
     3901 cgtagtagaa ggccgcgcgc acggtgtcgg ggtcgcggcc ggtcaggcgt gcgtaggcga
     3961 tgcgataggt cgcgagctgg acggcggcgg cgtgggcctc ctgcccccct ggcggggcgc
     4021 cggacttcca gtcgacgatc gtcaccgacc cgtcgggttc gaggaagacc gcgtcgatcc
     4081 gcccgcggac cgccgtgccg gccacgaccg tctcgaccat cacctcgacg tccaggggct
     4141 cccgctcggc ccagtcgctg gcgcggaagc gggcgatgag gtcctccagg tccacctgtt
     4201 cgccggtgtc ggggtcggcg ctgccgggca ggtcgtcggg ctcgagcagc gcggcgcggc
     4261 ggtagtgccg ttcgatccag gcgtggaatg cggtgccgcg gcgggtcgcc acgctcggcc
     4321 gttgcggcat cgggcggcgc acgccgacgg cgaagtcgtc gggttcggcg gcgaaggaga
     4381 ccagttggga ggtgctgacg tgcccgccga gggcgtcgag gagccgccag gggccgccgg
     4441 gtgcggactg gacggggaag gaaccgctgc cgtcgatccg ggcctgccgt tcggcgacga
     4501 ggacggccac ctcccggggc agcacgtggt cgctcaggtc gggtcggccc atggccgcgg
     4561 ccaccgcgcc cgctccctcc cggaccgggt cggcgacggg cgcgtgcggc ggccagctca
     4621 ccgagaccgg ttcggcgagt gcgggattgg tcgcatcggc cggcgggtcg gggcaccagc
     4681 cgagggtcgt gaggaggtcg gggcgctgtt cgcgcagttc gaggaggaat cgcgacggga
     4741 cgcgcggggt cttgggccca ccccagacgg cgcacgtcag gagcatcgcc cggcgcgcgc
     4801 gggtcagcgc gacataggcg agccgccgct cctccgctat gcccgccgcg ccactcgcga
     4861 cgcggaaggc cgcacgccgg tcgtcgagtt ccttggcgtc ggcggcgctg cgccaggcga
     4921 aggccggcaa cgaggcggcg tcaccgcgca ggtcatacgg cagcccgtcg agcccgatcg
     4981 tccacgcccc gtcgccgggc tcgctgctgc gccaccggcc ggtgtcgggg tcgaacttgc
     5041 tggtcgccga gccgtgggcg ggaaacccgg actcgaccag acccgggatc gccaccgcat
     5101 cccattcgag tcccttggcg gcgtgcacgg tcagcacgtg cacggccccc ggcacgatgt
     5161 cgaggacacc gcggtcgagg ccgcgttcct cggcgagggc ggcatcgagc caggccagga
     5221 agccgcccag ggtcggccgg tcgccgccgg cggcgaagtc ggccgcgaca tccgtgaagg
     5281 cgtccaggtg ggcccgcgcc accccgacgg ggtagcccgg ccgggccagg atctcgatgt
     5341 cgagaccgag agcccgctcc gcctcggcga cgatgtcgac caggccggac ccggtcaccg
     5401 cccgcacccc cgccagtgcg gcggccaggt ccaggagccg gcgcacggct gccgggctca
     5461 ggccggtggc ggcccgctcg gtcagcgatg ccgcggccag gtcgacgaga ccctcgacca
     5521 ggctcacccg gtcgctcgcc gcgtcggaaa gatcgctgcc gcgactgctg gccccccggc
     5581 gccggggcgg atgctggcgc gagcgcgccc acgcggcgaa ggcgtcgagg tcggcggcac
     5641 cgagccggca caccggcccg gtgagcagcc gcatcaaccg gtcgccgcgg ccggcgtcat
     5701 gggcgatcgt gagcagggcg acgacatcct ggacttcggg cgtcatcagc agcccgccga
     5761 ggccgacgac ctcgacgggc aggccggcag cctcgagggc gtcgacgacc gccgggaact
     5821 gggatcgttt gcggcacagc acggccgccg actgcgcccc ctcggcgcgg cgctgcgcga
     5881 tccagtccgc gacgagggcc gcctccgcgt ccaccgtctc gaggcgggcg acctcgatgc
     5941 ggccgtcccc ggcagcgggg gctgccgaga ggggttcgac ggggaccaca gccccggcgc
     6001 gcagcggctc ggcgacggtg ttggcggcgt cgaggatcgc gcgatcgttg cgccaggtca
     6061 ccgacagcgg cagcaccgag accgaggcgg caccgaagac cgcggggaac tgcgcgagtg
     6121 ttgccgcgct ggcgccgcgc cacgcgtaga tggactggtg cgggtcgccg accgcggtga
     6181 cgcagtggcc gggcccatcg gcgccggccg gggcgaagag ggcggcgagc aggtgcaact
     6241 gcgcgtgcga ggtgtcctgg aactcatcga gcagcactga actgaatcgt tcgcgctcga
     6301 tgcgtccgac ctcggggaag cgcatcgcga gttcggcggc caccgccatc tgatcggcga
     6361 aatccatggc gccgtggtcg cgcttgagcg cggcataccg ctgcagaacg gggaccaggc
     6421 atcgcttggc gcgcaacgct tttcgcatcg ccttgctctc ggcaaggagc gcccgtccgg
     6481 cgccctcgat gacctcgagc cgctcgtcga aaccggccag ccaggcatcg agctggtccg
     6541 gcgtgaccag gtgctcggcc atctcgccgg cgagatcgat cacggcattg gtgatggtcg
     6601 actcggcctt gccgatctcc tcgagcgggc cctcgtcgtt gctgacgacg gcgtgggcgt
     6661 actgccaggc cgccgcctcg gacagcaggc gggcgtcggg ctcgcggccg aggcgcaggg
     6721 cgtgctcgcg gaccagccgg ccggcatagg cgtgataggt ggagatcgtc ggcggggcgg
     6781 cgacgacccc gtccgtctcc tccggccggg ctccggcagc gccgtcgatc acccccgcgg
     6841 cggtcagcgc gcggaggcgg gtgccgaccc ggccggccag ttcggcagcg gccttgcggg
     6901 tgaaggtcag cccgaggacc tgctcgggct cggcgtagcc gttggcgacc agccacacga
     6961 cccgggcggc catcgtctcg gtcttgcccg agccggcgcc ggcgaccacg agcatcggcg
     7021 ccggcggatg ttcgatgatc tcctgctgtt gctcggtcgg gaccggcagg cgcagggcgg
     7081 cggcgatctc ggcagcgctg atccgcgggc ctgccggggg cggtgtggag gagggggtca
     7141 tgtgagcatc ccgccttcgg gtcgcgccgg gcaggacgtg gcgaccgggc aggtgcggca
     7201 gccctccccg acggtggcgg tgaatcgcga cccggccatg gcgtgcgccg cgtcggcgac
     7261 ggcgtcgtgg gcccagcccg gctcgggatc gtcggcgagc ggggtctggg cctggacgga
     7321 ggtcgcagcc ttggtcgtgg cggccttgcc gagctggacc agcacggcgc cggcaccgat
     7381 ggcgtcggga ccgaaggcgc cttcggccac tgcgacctga taggcgccga gctgcggatg
     7441 gcgggcgagg tcggcggcgg ccggtttgct gctgccggtc ttgtagtcga tgacgcggat
     7501 gcggccgtcg tcgtcggtct cgagccggtc gacgatcccg ctgatctgcg cccggcccac
     7561 ggtggccgtg aactgctgct cggcagcgag ccgccgccag ccgagggagg cggcgtgctc
     7621 gaagtatgcc gccgccctcc cgagcatcgc ctccgcctcg gcccgcttgg cccgggtgat
     7681 ccagcccgcc ggcaggccca gccgcccgaa ggagagatcg agccggccac cgagcgcggc
     7741 ggcgtcggcg tcgcccccgt cggccaggac ttcgtgcacc agggtgccga gcgcggccga
     7801 tccgatggcc gggccgtcac cgcccgcgcg ggtcagcagc caacgcaacc ggcacgaggc
     7861 gaaggtgacc agcgacgacg gtgagaccgg cacgaacgcc gaacccggca cccgcggtcg
     7921 gtcgtcggtg agcgcgcgca gcgcccacca cgactcgggg tcggcaccgc gaacgccgac
     7981 ggcggcgagc cgggcgagcg tggcggcctg ttgggcctgc gctgcccgct cggccggcgc
     8041 ggcccgcgcg tcggccggcg cggtgacggc gcgccgcagt gcggcgacca ccccgacgag
     8101 ggtcagcggc gtggcgacct cggtcagcgg tcgctcggtc acggcgccgt ccggagcgac
     8161 gaccgggggc gcgatgacgt cgagataggg cgagggcatg tcgtcgccgt cgcgcaccgc
     8221 gctgatatcg acccgccgcc gggtgcgggt caacgccaca tggaacagcc gggcctcgtc
     8281 gtgccgcacg acctgctggg cggcccggat cgagcgttcg cggcccgcga gcgcgtcgag
     8341 gagatcctcg gacccgagca gcgaaccgcg cagccgcagg tcgggccacg cgccctcctg
     8401 caccccggcg acgaagacga tctcccagct gcgcccggcg gccgcgtgcg gcgtgaggat
     8461 gctgacccgg tcacggtcgg cgccgcgggt gacgatggtg tcggccgcga catcctgccc
     8521 ggcgaggtgg tcggcgaagg ccgcggcccc gatgccgggt cgccggtcgg cgaagtctgc
     8581 tgcggccgcg agcacggcga tgacggcatc gaggtcccgg tcggcccggg cgccgacccg
     8641 gccaccggcc agcgccgcgc gccgccaggt gtcggcgacg gcggagcgct cccacatcgc
     8701 ccagagcaca tcctgggggg tcgccgcctc cgcggcgagc gccgccgtcg ctgcgtcgag
     8761 catgcgggcg atccgccgcg ccccggcccc ctcggtgccg agctcgctca gttcgccggg
     8821 tcgggccagg gcccgggcca ccagttcgtc ggcgggcgca ccctcgccgt tgtcgatagc
     8881 gatgcgccgc accgcccgcc gcacccgccg cagggtgacg gcgtcggcgc cggcataggg
     8941 gctggtcagc aggtcgatcg cggtctcggg cgcgagccgc cagccgccgt cggggtcggc
     9001 ggccagcacc tcgggcgcgg cgtccgcgcg gaccgccacc cgttccgcgg ccgcgcgcag
     9061 cagctgcagc agggggcgca ccgcatcctc ctcgccgagc gggatgtcgc tctcggcgtc
     9121 ggcgaccggc acgtgggccg cggcgagcac ccggcggacc acgtcggatc tgccctggcc
     9181 gcggacgagg accgccatat cgcgccaggc cacgccgtcg agcaggtgcg cccgccgaat
     9241 tgcgtccgcg atataggtgg cctcctgcgc cgccgaccgg aagacgttga cgttgacctc
     9301 gccggccggg ccgccggcca cccagcgccg cccgtgcgcg ccccccagag ccccgatcag
     9361 ggcggtcacc cgccggtcgg ctttcgccac ctcctggggc agccggtatg ccgtgccgag
     9421 gacgaccgtg gttccctccc ccaggtcgcg ccagccctcg gccaggaagc ggggatcggc
     9481 cccgcggaag gtctgcacgg cggcgtcggg gtcgccgatg agcacgatcg cgatgcccgc
     9541 gcccgcgagc agcccgagca gccgcgcgcc cggcgcaccg acctcttggg cgtcgtcgac
     9601 gatgacgacc cggatctggt cggcaagctc cccgagcagc tccgggtcgc tctccagcgc
     9661 atcgcacgcc cccgacagca gccacgccgg gtcgaaccct cccggccgcg ccagggcggt
     9721 gacctggtcg tattcgtcga gcaccgcccc tgcggccgtc cactccgggc gatcgtgtgc
     9781 gcggccgagg tcggccaagg tggccgcgtc gaggccgtgc tcgacggccc gcatgagcag
     9841 atcgcgcagt tcggcccgga agccgcgcgc cccggcggcc tcccccaagt gctccggcca
     9901 gtccggcgcc cgggccggct ccgcgacgtg gccggccagc aactctcgca ggatggcatc
     9961 ctgctcggcg ccgctgagca ggaagggcgt gggcaggtcg tcacggatcg ccgcccgccg
    10021 caggatcccg aacgccagcg acgcgaaggt ccgcgccagc ggggtgcggg tcgaggccgc
    10081 gacccgccgg gtcacctgct cccggactgc ggccgcggcc cgccgcgtcg gagccagcac
    10141 gaggcactgc tcgggcccgg cgaccccgcg tgccacccgg tcggcgacga gttccaccgc
    10201 gagcgtggtc ttgcctgtgc ccggaccccc cagcacccgc aacagccggc cgtcgtgggc
    10261 gagcgcacgc tgctgctgcg cgtcgggggc gaaagtgcct gcggcggcgg cgcgaagtgc
    10321 ggtcgctgtc atacgaaaca gtccaccatc gagcaccgac aacccccgtc aggcagcccc
    10381 ggcgtgtccc ccgtgcgcgg cgaatctaca tgtcaaccgc gctggtcagc cgggctggtc
    10441 agccaactcg ccgggccagg cgctgatccc accctcgagg tcggtgacgt cggtgaaacc
    10501 ggccgcccgc agccgctgcg cccccgcggc ggaccggcct ccggcggcgc agtacaacac
    10561 cacccgcgca tctcggtcca ccgccagttc ctcgatccgc ccgacggggt gcagcagggc
    10621 gccggggatg atcccgcccg cccattccgc cggctcgcgc acgtcgacga gcacgacgcc
    10681 cgggtcgctc gccagcacct gcgccagctg ctcggcggta tgccgcgggc ccccggtctc
    10741 gcaggccgcc gactcgtccc ggggtgcggt gatggtcgcc tccggcccgc agaggcggca
    10801 cgccgggttg cgggcgaccg ggacggtgga ccaggtcccc tcgagggcat cgtggatgag
    10861 cagccggccc gtcagcggac gcccgacgcc actgagcacc ttgatcgcct cggtggcctg
    10921 gatcgacccg accgccgcac acagcgaccc gagcaccccc gcctccgcgc acgaggccac
    10981 gctgcccgcg ggcggcggcg ccgggaagac gcagcgatag cacggaccgt ggggcggcca
    11041 ccagacgctc acctgcccgt cgaagcgcag gatcgacccc cacacgtgcg ggcggccggt
    11101 caaggcgcag gcgtcggcga cggcataccg ggtggcgaag ttgtcggcgc cgtcgacgac
    11161 gacgtcatag cccgacacca ggccgaccgc gttggcgccg gtgagccgca acgggtggcg
    11221 ctcgacccgg acgagcgggt tgatcgccgt gagccggtcc gcggcggagt cgaccttggg
    11281 ccgcccgatc gccgcggtgt cgtggatgac ctggcgttgc aggttcgact cgtcgacgac
    11341 atcgtcatcg acgatcccga tcgtgccgac acccgcggcc gcgagatagg tcgccgtgac
    11401 cgagccgagc ccgccggcgc cgatgatcag tgcccgcgat ccggccagcg cttgctgccc
    11461 ggccgcgccg aactccggca ggaggaggtg gcgggcgtac cgccgccgct ccgcgggccc
    11521 gagctcagga ggcgcgggcc gatccggcgg gcgggtcacg gcagacccga agtagctcgc
    11581 ggttcgaaat ggcacgtcac cgtccgagcc tactcactag tattggcgcc accgggtcag
    11641 tccgcgcgcg cggaccaacc acccggcaga cgaccggagg gcgatacatg gcacgcgaat
    11701 tcaccgcggc cgacaccacg cgcgggcaac gtctgccccg cctcgagcgc cgtgcccagc
    11761 tgctcgaggc cgcccagtcg gttttcgtcg acaagggcta ccacgcgtcg ggcatggaag
    11821 acatcgccga gcgggcaggg gtctccaaac cggtcctcta ccagcatttt ccgggcaagc
    11881 tggagctcta tctcgcgctc gtcgacaagc acacggccgc cctcgtcgag ctcgtcgagc
    11941 tcgtcgaggc ggccctcaac gagagcgacg accacaagga ccgcacctac gcgagcatcc
    12001 gggcgttctt cgacttcgtc gcgcgcgagg acgagccgtt ccgcatcgtc ttcgagtccg
    12061 acctcaacga ggtcgcagcg gtgcgcgaac gcatcgggtg ggcgaccgag gcctgcgtgg
    12121 acgccatctc ccggcgcatc atgctcgaca ccgagttgcc gcaaccggtg gcccggctgg
    12181 tggcgcaagg gctcgtcggc atgtcccagg tctcggcgcg gtcctggttg cgggacggga
    12241 cgatcccgct cgacctggcg gtcgagatca tcggccggct ggcgtggcgc ggcgtcggtg
    12301 gccaacgggc gcgcctcgtc gcccccgcct gacccgggcg tccaccccgg gtgggaatgc
    12361 cacggggcct ggtgtgtttg acttctggca accgcctgtc cgatcgcgtg ttcggggtcg
    12421 tccccggcag ccgggccggc caccgaccaa ggagcaaccg tatggaagtg aagatcggca
    12481 tcgcgaacgt cgcccgtgag gtgagcatcg agccggagga gagcgcgtcg cgcgtcgagg
    12541 aactggtccg ggccgccgtc accgacggca ccccgctgcg cctcgaggac gccaagggcc
    12601 gggtcgtcat cgtccccgcc gccgccatcg gctatgtcga catcggcgag tccctcaagg
    12661 gccgggtcgg gttcggggcc tagccaccgt cccgtcggtg gctttcaccc gtggcccttg
    12721 cgcgggcgcc acatcggtaa caaccgcacc gctaatccgc atggaacggt cctcgccatg
    12781 cgatggttga gcgtgaacgg atccgaatcc gcgggtccac gattgccccg accactgggc
    12841 acatttcgaa aggagcggtt cctgtgatcg gaaccatcat cggtgccatc atcgccggcg
    12901 ccatcatcgg cgggctcgcc cgcctcatcc tgcccgggcg ccaggggatc tccctccccg
    12961 tgacgatcat cttgggcatc ctcggctcgc tgatcgcgtc gtggctcgtc tacaacctcg
    13021 gctacgacaa cgagaacggc ggcttccagt tcatcccgtt catcgcgggc gtgctcgtcg
    13081 cggccggcct catcgtcggc tacggcgcca tgaccggcaa caagcaggtt cgctgaccag
    13141 ctgcactgat ccagccacac cgcatgagcg gccgggccct tcagggtccg gccgcttcgc
    13201 ttgtctgccg atcgccccga gccgatcacc ccgagccggt caccagggcg acatcccggg
    13261 tggctacgct cgtcatccgt gtccgacgtc tcctgccccg actccgccga ccggaccgcc
    13321 accgtcgacc tgctcggcat gctcgccctc ggcgagctga ccgccttctc gcggctggcg
    13381 gccgacgccg acatggcgcc cgccgtggcg ggacgggagg ccttcgcccg gctggcgctc
    13441 gtcgagttcg gtcactacga actcctcctc gcccgcctgc gcgatctcac cggcgcaccg
    13501 gaggcggcga tggcgccgta cgcccccgtg ttcgcggcat accacgaacg cacccggccg
    13561 ggcacctggc tggaggcggt ggtcaaggcc tacgtcggcg acggcatcgc ggccgatttc
    13621 tcgcgcgaga tcgcccgatt cgtcgatgcc gagtcgcggg cggcgatcga ggcggcgatg
    13681 gacgatcgag agcgcagcgc ggtgctcgtc cggctggccg cggagggtat gcagtccgac
    13741 ccgcacgtcc gcggccggct ggcgctgtgg gcgcgccggc tgcacggcga ggccgtgtcg
    13801 caggcgcaga ccgtcgctgc ccagcgggag tcgctgacca ccctgctcac cggctccggc
    13861 ccgcagctcg atctggtggc cctcggcgag ttgttcgcgc ggctggccag gcggcatacc
    13921 gaacggatgg ccgccctcgg cctggaggcg tgagcggccc cggacgtggc cgcgcccggc
    13981 taaggctaga cttagccgcg taagcacgtg gtgcccggtc ggcacgaatc atgcgctggc
    14041 acacggccgg cgaccccgcg cgagacgctt ccgtctcggg cacgaagacc tccgatcggc
    14101 tccttccccg gcccgccgcc tgcgcggcga ggtcccgatc gagagatgaa taacgttgtc
    14161 cgacaaaact ttcgccgatt tcggcgtgca ccccgatatc gtcgccgccc tggccgagca
    14221 cggcatcgtc acccccttcc ccatccaggc gatgacgctg ccggtcgccc tcggtggcca
    14281 cgacatcatc ggccaggcca agaccggcac gggcaagacg ctgggcttcg gcatcccgat
    14341 gctgcagcgc gtcgtcgccc cgggcgatga ggggtatgcc gccctgccgg cccccggcgc
    14401 cccgcaggcg ctggccgtcg cgcccacgcg tgaactggcc gtgcaggtcg ccaaggatct
    14461 cgagcgcgcc ggcgcccagc gcggggtgcg ggtgctgacc gtctacggcg ggcgggccta
    14521 cgaaccgcag gtcgaggccc tgcgcaaggg cgtcgaggtc gtcgtgggca ccccgggacg
    14581 gctgctcgac ctcgccggcc agggccacct caccctggcg cacgcgcgga tcgtcgtgct
    14641 cgacgaggcc gacgagatgc tcgacctcgg cttcctgccc gacgtcgaga agctcctcgc
    14701 cctgacccct gccggccggc agaccatgct cttctcggcg accatgcccg gggccgtcgt
    14761 cgcgctcgcg cgacgctata tgacccagcc gacgcacatc cgggcgatga gcgaggaggg
    14821 ggagaatcag catacggtca aggcgatcga gcagttcgtc tatcgcgccc acgcgctcga
    14881 caagaccgag atgctcgcgc ggatcctgca ggcccgcggg cgcggcctga cgatgatctt
    14941 cagccgcacc aagcgcaccg cggccaaggt cgccgacgaa ttgaccgagc gcggtttcgc
    15001 cgcggcgtcg atccatggcg acctcggcca gggcgcgcgc gagcaggcgc tgcgggcgtt
    15061 ccgctcgggc aaggtcgacg tgctcgtcgc gaccgatgtc gctgcgcgcg gcatcgacgt
    15121 cgacaatgtc actcacgtcg tgaacttcca gtgccccgag gacgagaaga cctacgtcca
    15181 ccgcgtcgga cgcaccggcc gggcgggcaa caccggcgtc gccgtcacct tcgtcgactg
    15241 ggacgacatc ccccgctggg gcctgatcaa caagaccctc gacctcggca tccccgagcc
    15301 gcaggagacc tactcctcct ccgagcacct ctacaccgac ctcgacatcc cccgcgaggc
    15361 caagggcaag ctgcccaagt cggcgcagac gcgcgccggc ctggccgccg aggagatcga
    15421 ggacatcgag ggtccgtcga agaaggccgg cggccggggc ggctcccgta gcggacgggg
    15481 cgggcgcacc gggacccgcg acggcgctcg cgagggccgc agcggtcagg cccgggctgc
    15541 gcgcgatggt gccgctcccg agggtcaggc cgaacggacg ccgcgctcgc gcagccggcg
    15601 gcgcacccgc ggcggcaagc cggccgccgc aacaaccgac tgaggcgact gacacgaggc
    15661 cgggagggag cgaggcggga ggggtagtca cggcccgagc gtcgctctgc gaccgagggt
    15721 cgtgtctcct gccccgacgc ctcgctcaca cccggccggc tttgggcacc gcctactcgg
    15781 gcgtgatcgt cgaggtgtcg atgacgaagc ggtagcggac cttggaggcg accacgcggt
    15841 catacgcagc atcgacctcg tcggcggaga tcgtctcgac ggtcgccgcg atgtcgtgct
    15901 cggcgcagaa gtcgagcatc tcctgggtga tcgggatgcc gccgatgttc gacccggtga
    15961 gcaccttgtc gcccatgatg agcgagaaca cctggaagtt ctgcggcttc tcgggcagcc
    16021 cgacgagcac gagcgcgccg aagggcttga ccatgccgag gtacttgtcc agcggcaggt
    16081 cggcgctgac ggtgcagacg atcaggtcga agctgttgcg cagttccttg aagacgctgc
    16141 cgtcctccgt cgcccgatag tcgacggcgc cgaacgctcg cccgtcctcc tccttcgaca
    16201 tcgtctggga gatgacggtc acgtcggcgc ccatggcggc ggcgatcttg acacccatgt
    16261 gccccaggcc gcccatcccg acgatggcga ccttcttgcc cggcccggca ccccagcgct
    16321 tcagcggggt atagagcgtg atgccggcgc acaacagcgg ggccgcgacc tcgagcgaga
    16381 tcgcgtcggg gatgcgcagc acgaaccgct cggtgacgat gatctgctgg ctgtagccgc
    16441 cctgggcgat ctcgccgtcg tagttcttgg agttgtaggt gccgacggcg cctttctcgc
    16501 agaagttctc gctgcccttg acgcagtagt cgcactcgcc gcacgagtcg acgaggcagc
    16561 cgacgccgac gtggtcgccg accttgaact tcgtgacctc ggaaccgatc tcgctgacca
    16621 ccccggcgat ctcgtgcccg gtgacgatgg ggaagatgca cggtccccac tcctcgcgca
    16681 ccgtgtggat gtcgctgtgg cagatgccgg cgtacgcgat gtcgatgcgg acatcgtccg
    16741 ggcgcaggtc gcgccgttcg accagcccct ggcggaaggg ggcgtcagcg gagtctttga
    16801 ggatggcggg gacctgggtc gtcataccct ccaacctacg cgactcacag acgggtgagg
    16861 atgtccttgg ccacggcccg gtaggcctgc gcccccttgg acgtgcgcga ggtctcgagg
    16921 atggaccgtc cgacggcggg cgcctcggcg aagcggaccg tgcgcgggat gggcggcgcg
    16981 agcacgggta tgccgtagcg ctcccccacg tcggcgagca cgtcctgctg gtgggtactg
    17041 cgcccgtcgt acatcgtcgg cagcacgccc acgacgtcga gcttcttgtt gaggatgcgc
    17101 ttgacgtcgc gcaccgtatc gagcagctgc cccacgccgc ggtggccgag catctcgcag
    17161 ggcatcggga cgatcaggcc gcgggcggcg gtgagggcgt tgagcgtcag caccccgagg
    17221 ctggggctgc agtcgagcag gatcaggtcg tatgccgtcg ccaccggcgc gaggagttcc
    17281 tgcaggatgt attcgcgccc gggccggccg aggaggaccg actcggtgcc ggcgagttcg
    17341 atggagctgg ggatcaggtc gacgccgtcg ccgcactcga agatgacgtc ggcgagcggc
    17401 accgcgccgg tcagggcgtc gtgcaccgac gcctcgacct ggtccgggtc gatgcccagg
    17461 ctgaacgtca ggcaggcctg cgggtcgagg tcgacgagca gcacccgctt gcccagttcg
    17521 gcgaaggcgg cgccgagcga ggcgaccgac gtcgtcttgg cgacgccgcc cttctgattg
    17581 gcgacggcca ggatggtcgc gcccttggcg cggctacggg gcggcatatc cccaagtctc
    17641 gcagcggcat acggctgcgc ggggcaggga cacggcatcc cggggatgtc gtaggcgtga
    17701 tccggcccac cacggggcgg gggtatgccg cgcgatcagg ccccacgtcg ccccgcctcg
    17761 gcgagcgcga atcggcgcat caggtcggcc gccggcagca gccggcgctc gctgagccag
    17821 gcgatcccga cctcgcgcct ggcgtcgttg tcccgcaacg gaatcaggcg caccgggccg
    17881 agttcggtcg gcacggagcg gcccagtgcc ggcacgacgc cgacgccgag cccggcggcg
    17941 acgaacccgc gcacggtcgg cagatcgtcc acttcgaagg cgatgtcggg ctcgaaaccc
    18001 gctgcggcac aaagctgcaa gacctgcgcg cgcatctccg acggcgcccg gcggatgatg
    18061 aacggctccc gcgcgacatg ggcgagctcg gtctcctcgg cgccggcgag gcggtggtcg
    18121 agcggcacgg cgagcagcag gggctcgatc gcgacctgac gccactccac accgggcccg
    18181 gacacccggc cggtcgtgat ctccaggtcc acctcccgcg tcgccaggag gcgcgagatg
    18241 gcgccgaccg cgccgacgga cgagcgcgcc acgcgcaccc gcaccccggg atgagcggcc
    18301 cggaagtcgc ggatcagccc gggcacgaac cacgtgccga ggctgagcgg gtaggccagg
    18361 gtgacgatgc cggtgccggg atcgaggagt tgttcgacgg cggcgacggc gtcgtcgtac
    18421 tgcgccatca tcgcgtcgag gtgcgccttg aagaccgcgc cggcggcggt caggcgcagc
    18481 acccggccgg accggcgcag caatggggcc cccagttcct cctcgagtcg cgcgagggca
    18541 cgcgagacgc cgggctggct cacggcatac gtctcggcca cctcggtgac cgtcatcccg
    18601 tcggcgacat gctgaaacca gcgcagcgtc tcggtctcca tgaccaatga cgttagcgca
    18661 tgggttctat gcatgcgagc tattggacgc attaattgaa agggcgcaca attgaggaca
    18721 tgagcatcaa gaacatccgg atcagcctcc ggcaccaccg tgcggccgtg tcggcccgtc
    18781 aggacatgct gcgccagctg tccgtctaca ccacgcccgc ggagatcgag gacatgctgg
    18841 ccgccgtcga cggccaggac agccccgacg ccgacctcat gcgcgaggtg ctcggcgaca
    18901 agctcgcccg cgcctaccgc gactccgccc gccccgcctt cgggatgcac gtcgccgcct
    18961 gatcggcgtc cgcccgcact cccccgacat cgcaccgcgc ccgacctggg ccggtgcgat
    19021 tgtcatgtgt cagcgcatag ggtgatcgca tgtccactcg ggtgtatatc gcgtccgggg
    19081 aggcgcattc cggcaagtcc gccatcgcca tgggattggt ctcggcgctg gcccgccatg
    19141 ccggccgggt ggggatcttc cgccccgtcg tgaccgatcc cgaggccgat ccggtcctga
    19201 ccctcctcgc ctcccggctc gaacggccga tcgacctgcg cgcggcgtcc ggcgtgatgc
    19261 ttcaggatct gcacgacgac cgcgatgctg ccatggcgga ggtcgtcgca cgcttccacg
    19321 cggcggcggc cgacaccgac atgatggtca tcgtcgggtc cgactacagc ggagtgcccg
    19381 cccccaccga gttcgccgcg aacgcctctc tcgcagcgca tctcgacgcc ccgatgatcc
    19441 tcgtggtcaa tggtgccggg cgcacgcccg agcagatcgc cctcgcggcg gagctgatgg
    19501 ccgagggcgc gcacgcccgg catgcccagg tgctggccgt catcgtcaac cgcgtcgacg
    19561 gcccggccga ggcgatcgtc gccgcgaccc agcaggcgac ccgcggcata ccgacctaca
    19621 ccgtccccaa cgtcccgatc ctcgacgcgc cgaccgtccg cgagctcatg ggcgcggtca
    19681 acggggaact gctctccggc gatcccgccc ggctcgacaa cgagtcgctc ggattcgtgg
    19741 tggcggcgat gaccatgccg cacgtgctcg accggctgtt gccggccgga gccgtgatca
    19801 cccccggcga ccgcgaggac gtcctgctcg gcgtgctgct ggccgaccgc ggcaagacct
    19861 tcccgagccc gtccgccgtc ttcctcaacg gtggttttac gccgagtccc caagtgcagc
    19921 agttgatctc gggtatcgac tcgagcctgc ccgtcatctc ctgcgcgggc gggacgctgg
    19981 ccacgaccgc ggcgctgacc ggggtcaagg gctcgctcaa ccccgcctcg gtgcgcaaga
    20041 tcgcggcggc cgagaaggtc ctcgcggaga gcatcgacac cgacgcactc ctcgagcgcc
    20101 tcggggccgc atcgcacggc gcggtcacgc cgctgatgtt cgagcagaac ctgctcaccc
    20161 aggcccggaa ggccggcaga catatcgtcc tgcccgaggg ggatgacgac cggatcctcg
    20221 aggctgccgc gctcatcctc gagcggcagg cggcccggct gaccatcctc ggtgatcccg
    20281 cccagatcgc cgctcgcgcg cacgctctcg atctcgacct gaccggtgcg cgcctgttgt
    20341 cgaccaccca ggagcccaat ctcgaacgct acgcgcagga gtacgcgcgc ctgcgtgccc
    20401 acaagggcgt ctcgatcgac atcgcccgcg accgggtcat cgacggcgcc tatttcggca
    20461 ccatgatggt gttgctcggc gaggccgacg gcatggtctc cggcgccaac cacaccaccg
    20521 cgcacacgat ccgcccggcg ctcgaagtgg tcaagaccgt gcccggtgtc tcggtcgtct
    20581 cgagtgtctt cttcatgtgc ctggccgatc gcgtgctggt ctacggcgac tgcgccgtca
    20641 accccgaccc gacggccgag caactcgccg acatcgcggt ctcctccgcc gcgaccgcgc
    20701 gccagttcgg catcgaaccc cgagtggcga tgctctctta cagcaccggc gggtccggca
    20761 gcggcctcga cgtggagaag gtgcgaaccg caactgacct tgtgcgacaa cgcgatccgg
    20821 agctggctgt cgaggggccc atccagtatg acgccgccgt cgaccccgac gtggcggcga
    20881 ccaagctgtc cggcagcaag gtcgccgggc aggccaccgt gttcatcttc cccgacctca
    20941 acaccggcaa caacacctac aaggccgtcc agcgctcggc ccacgccgtc gccgtcggtc
    21001 cggtgctcca aggattgaat cgcccggtca acgacctctc ccgcggggcg ctcgtgcgcg
    21061 acatcgtcaa cacgatcgcc atcaccgcga tccaggcagg agcttgagcc atgacctccc
    21121 ccgacggccg cgtcctcgtc gtcaacgccg gctcctcttc cgtgaaatac caactgctcc
    21181 agtccgattc gggcgaggtc ctggccaagg ggctgatcga gcagatcggc gaatccagtg
    21241 gcggcaagca ccggcatacg gtcgccggca ccgacaacac ccgcaccggc gaaatcggcg
    21301 acatgacagc cgccctcgcg tcgatggctg cggctttcgc cgacttcggg ccggacctgg
    21361 acgccgagcc gcccatggcg gtcggccacc gcgtggtcca tggcggcacc cgcttcagcg
    21421 cgacgacccg catcaccgca gcgacgacgg acgccatccg cgacctgatt cctcttgcgc
    21481 cactgcacaa tccggcaaac ctcgccggca tcgaggccgc gctggcgcag ttccccgacg
    21541 tgccgcagtt cgccgtcttc gacacggcgt tccactcgac cctgccggcg gcggcataca
    21601 cctacgcggt gccgaaggtg tggcgggagg actatcacgt gcgccgctac ggcttccacg
    21661 gcacctcgca tcactatgtc tcagaacggg tttcggcgct gatgggccgc gccgacctcg
    21721 ccacgatcgt gctgcatctg ggcaacgggt gcagcgcgtg cgccgtgctc gacggcgaga
    21781 gcgtcgagac ctcgatgggc atgacgccgc tcgaggggct ggtcatgggc acgcgctgcg
    21841 gcgacatcga ccccgccgtg cccttccacc tcgcccgcac cgagggcctg gacgtcgacc
    21901 agctcgacac cgcgctcaac aagcagagcg gcctgctcgg gctgaccggt gacaacgact
    21961 tccgcaccat cgccgaacgc gccgccgccg gtgaccgcga ggcgcaagcc gccatcgacg
    22021 tcgtcgtcca ccggctcgtc aaatacgtgg gtgcgtatgc cgcggtgctc ggccggctcg
    22081 acgcgatcgc cttcgcgggt ggcatcggag agcacaatgc ggccctgcga gccgcggtgc
    22141 tgggccgcct cggcatcctc ggggtcgagg tcgacgcggc ggccaacgag ggcgccgacg
    22201 gggaggcgct gctgacgacg ccggccagcc gggtcgcggc cttcgtcatc cccaccaacg
    22261 aggagttgca gatcgcccgc gagtgcctgg agcacctgga gagtccgtga gccgcaacac
    22321 ctggtcgggt cagcgcctgc cccatccccc ggggcggcgc cggttcgtcg gggacatcga
    22381 gcgcgaggcc gacccgaaca ccccggtgca gaacctcatg cgccgggtgc acgggctgcc
    22441 ggaactggtg gagttcaagg cttttcgtga gaagttcgtc tttgcgttga gcgcgcgcgt
    22501 gtgcgcccag gtcgcggacg acgtgcactt cgacaaggcg ctggcacccg ccgtgctcgc
    22561 actacgccgc ttcagcggcg acgccctgtt caccgccttc actcacgaac cggcctgggg
    22621 ggtcgcccac gacctgctcg tccccgcgtt caccaaaccc gcgatgcgcg gctatcacga
    22681 cacgatgaac gccgtcgccg acgatctcgt cgcacggtgg gccgcgagca ccacgcccgt
    22741 cgacgtcgcc gacgacctga cccggatgac gctggagacc atcgggcgca cggcgttcgg
    22801 ccattccttc ggcaccttcg cccaccagga gcgcgacccc tacgtcgcgg ccatggtcac
    22861 cctgctctcc gactccggcc aggcagcagg gttgagcact ctgccactcg gccaactgat
    22921 cacccgccgg ccctcgagac gggcgcgggc ggcatacgcg acgcaggtcg agatcaccga
    22981 ggacatcatc gaggcgcggt tggccggcgg cgggcagcac ggtggcgggc agcaacgtgg
    23041 cggcgcggac ctgctcgggc ggatgctctt tcctgcacat gccgagtccg gagcccggct
    23101 cgaccggcgc aatgtcatca accaggtgct gaccttcctc accgccggtc acgaaaccac
    23161 ctcgggggct ttgtcattcg ccttgtggta cctcgccaac gacgatcggg tgcgggccgc
    23221 ggcccaagcg gagacggacg acctgctcgg gccggacccg cagtccacgc cgacgttcga
    23281 gatggtcccc aagttccgct acgtgcgccg cgtgctcgac gaggcgctgc ggctctggcc
    23341 gactgcgccc gccatcggac gcagtccgcg agcggtgacc acggtcgccg gctggacgat
    23401 gaccccgcag gactggctgc tggtcttcat cccggccgtg catcgcgatc cggctgtctg
    23461 gggaccggac cccgaccgct tcgaccccga caacttcctg cccgagcggg tgcgcgcccg
    23521 gccggcatac agttacctgc ccttcggtgt cggcacccgg gcgtgcatcg gccggcagtt
    23581 cgccctgcac gaggcggtgc tggtcctcgc gcggctgctg caccgcttcg acttcgctgc
    23641 cgacccggca tacgacctgc gcgtcaccga gcgactgacg ttgatgccca agggttttcg
    23701 gctgacggtc cgcgagcgga gcctggtggc ctgacgactt cgcgggattt atgggtcctc
    23761 ggggtgcccg cgaagtctcg gagcgacgca ggagcggaga acttcgtggg gtttaggaga
    23821 acacgacggt gcggtcgccg gcgagcagga cccgcgattc gcagtgccag cgcagggcgt
    23881 gggcgagcac gcgttcctcg cactcctccc cggcgcgcac gaggtcttcg ggcgtcattc
    23941 ggtggtcgac ccggatcgcg tcctgctcga tgatcggtcc ctcgtcgagg tcggcggtca
    24001 catagtgggc ggtcgcgccg acgagtttca cgccgcgttc gtaggcctgg tgatagggct
    24061 tggcgccctt gaagctgggc aggaacgagt ggtgaatgtt gatgatccgg ccagacatcc
    24121 gcgcggtcaa ctcgtcggag aggatctgca tgtagcgggc caggacgacc aggtcgacgt
    24181 cgagttcgtc gacgagggac agcagccgcg actccgcctc ggccttggtg tccggcgtga
    24241 ccggaatgtg gtagtaggca acgccatacg acgccacgag cggttccaag tcggggtggt
    24301 tggacacgac ggcggcgacg tccatgttga tcccgccgtt gcgccagcgg aagagcaggt
    24361 cgttgaggca gtgcccgaac ttcgacacca tgaggatggt ccggtatggc gcgtcggcgc
    24421 gccggagctg gaagtccatg tcgaaccgtt tgccgacggc ggcgacggcg ccgtgcaggt
    24481 cggcctgctc gatggtctcc cccgccaccg gctcgacgag cacgcgcatg aagaagcggc
    24541 cggtgcgcgg gtcgtcgaac tgctgactct cgaggatgtt ggctccgaga ttggccagcg
    24601 tcgaggccac cgcggccacg atgccgggcc ggtcggggca ggagagggtc aggacgaagc
    24661 gggtgggcac cgctggatag taaccccaga ccgggacgcc catccgtggt gggtgaacgc
    24721 gtcctaggct gcccccgggc gatcgatcga ggagaggggc gggtcgagtg gacagcaggg
    24781 agatcgcggt cgcggcgttc gcggtgccgg tgctgctcgc tgtcctcggg ctgtggtggt
    24841 tccgccggcg ccgccgcgac gagatcttcg agggcgtcac cccgggtgag ctgccggccc
    24901 tggggcagca ggtgacgcgc cgccgggtgc cgcgcggcag gcaggagtat gccggtccgg
    24961 tcgccgtccg gttcagccca ccggacggat tgactcccgg gctggtcggc accgtgatcg
    25021 acggacaggc gcacgtcatc gacgtcaccg ccaccatcat cgatctcgcc gtccgcggcc
    25081 acctgcggct ggtggccgaa gcggccgacc ccgacactcc tcccccgcca ccgcgggggc
    25141 cggcaccgaa aactcctgtc cgccagtccg atcacgactg gcgcctcata cgactgccgg
    25201 cgccggatgg cgatcggctg ctgccgttcg agcacaaact gctggacgcc gtgttccggt
    25261 acggccccga ggtcacggtc cgggaactga aggcccgtgg cttcgacctg acgatgcgcg
    25321 aggcgcagat cgggctctat cgcgaggtcg tcgaccgggg ctggtacacc aagcatcccc
    25381 gcgaccggaa ccggcggctc ggctgcctgg ggtggctgct ggtcgtcgcc gccgtcctgg
    25441 ccgcgctggc cgggatcacg gcccggccct gggagccggc acgcacgccg atgctggcgt
    25501 tgggtgcggg gctgctgttc gccgcgatcc tgctcatctg gggtggccgc gggcgcaccc
    25561 cccgcaccgc cgaaggtacg gccgcccgga tccaggcgct cggcttccgc gaatacctca
    25621 ccaaggccga ggcgaaccag atccgcttcg aagaggcccg cgacctcttc agccgctatc
    25681 tgccatacgc aatggtcttc ggggtcgccg agcggtgggc gcgcaccttc gccgacgtgg
    25741 ccgcctcggc tcggctgcag ggcgtgggcg acgccttctt cgacctgacc tggctcggcg
    25801 gattcgacgt gctcggcgac acgctcggcg ccggcctaca gatcgccgac gccgtcggca
    25861 gcgtcgccga cttctcggcg ggcatcgaca tcccggacct ggatctcgcg gacggtctcg
    25921 gcggcatcgc cagcagcgtc ggcgatttcg ccgactcggc cggtggattg ctcgacttcg
    25981 gcgacggttg ctccggctgc gacgggtgcg acttcggcat ctagcccgtc ccgtcaccgc
    26041 ggccaggtct cagtccggcg tcgagctgcc ggccaggagg gcggcggtga cgcccgtgac
    26101 ggcgaggagc agcaccggca cgccgacgag ggcgccattg ccgcggatga cgctgaaggc
    26161 gaccggcaca agcagcgcgt tgagcaccag ggtggccatc cgttgccact cctgcccgcg
    26221 cagccaggcc cgcgccatcg aggcgaggat cgtcgccccc acgagaaaga gcccgatcga
    26281 ggccagcgtg cggctcaggc tggactcggt ctcttcggcg atccccacgg catacccggc
    26341 cgcgaggccc accagcgcca gcgcctccag agcgaccacc gcggcagcca cccgcggcca
    26401 tcggccgcga ctcgccggcg gaccgacact cgaacggtcc ggttgcgcgg atgcggcctg
    26461 gtttgccatg ccccaagcct aattattgaa tcgggaacaa aagatatggc ccaaagcatt
    26521 ggcatatcgg cggtcggtcc ctgacaatgg tgagtcgacc accgaaacca ccgagggcct
    26581 cgctgtggcg cgcgccgcaa cccgagggcc gcccgccgct ggccactgac ctaggagatc
    26641 cacttcatgg attggcgcga tcgcgctgcg tgtctggacg aagaccccga gctcttcttc
    26701 cccatcggca acactggacc ggcaatcctg cagatcgagg aggccaaggc cgtatgccgg
    26761 cggtgtgagg tcatcgacac gtgcctgaag tgggccatcg agtccggtca ggactccgga
    26821 gtctggggcg ggctgtccga ggacgagcgc cgcgccctca agcgccgcaa cgcccgcgcc
    26881 cgccgcgccg gctgacgatc ccccggcggg caggaacgcg gacccgtgct caggcacccg
    26941 tgctcaggcc gggtccaggg ggcgcaggcg agcgaagaag cgcacccgcg tgccgtgcgg
    27001 ctgggcgtcc tcccaccgga tcgccccacg cagcccttgc acgagagagt tgacgatctg
    27061 ggtcccgagg ccggccttgc cgggacggaa cccggccggg atccccgtgc cgtcgtcctc
    27121 gatcgcgacc tgcagcacat cgccgtcgcc cccgtcctcc tcgacccgtt ccacccatag
    27181 ggtgaccgtg cctccggtct cgcgcaggcc gtgctcgacg gcgttctggg tcaactcgct
    27241 gatcaccagc gcgagcgaca tggcgtcctc cgcccgcagc cggccgaacg acccctcgac
    27301 ccgcgagccg atgggcacat cggtcgtcgc cacctcgacg atggcgtgca ggccccggcg
    27361 ggcgatctcg tcgaaatcga cggtctcgtc gaagccgtgt gagagcgtct cgtgcaccag
    27421 cgcgatcgtg ccgacgcgcc ggaccgcctc gtcgagcgcc gatcgcccgg ccccgtcggg
    27481 cagccgccgc gcctgcagac gcaagagcgc cgcgacggtc tgcaggttgt tcttgacgcg
    27541 gtggtggatc tcccggaccg tggcttcctt gctcagcagt tcgtgctcgc ggcgccgcag
    27601 gtcggtgacg tcgcgcagca gcacgatggc gccgacccgt tcgccgccct cggtcagcgg
    27661 tatggcgcgc agcgccaccg atcccgagcg ggtggtcgcg tcggcgcgcc aggccgcccg
    27721 ccccatcgtc accatcgcga gagcctcgtc gaccagcgcc tgatcgcgca gcacgtcggc
    27781 gaacaccgtc gccagctgct ccccgatcac ctcgcccgga tgcccgaggc ggtggatggc
    27841 actcatcgcg ttcggactgg catacagcac gatgccgtcc acgccgaggt ggatcacccc
    27901 atcgctgacg cggggggcgc cgcgccggct gcccgtcggc gcgctggcgc ccggccactc
    27961 gccggcggcg atcatccgca ccaacgcggc ggcgaggcct tgatagctga gttcgagccg
    28021 gctgggggta cgcagcgcgg tgaggttggt atgccgcgtg aggagcgcga tggtctggtc
    28081 gccgtcgcgc aggggcaccg cctcctcccg gatggcgtgg tcgttgtcga ggatggggcc
    28141 gccgcggcgc aggatggccc cctcgtcgag ggcgcgctcg agcagccggc tgcggtcccc
    28201 gacggcgtgc tggccgacga tgtcctcgaa gaagaccatc tgcccggtgt tcgggcgcac
    28261 gtgcgccacc gcccgccagt cgccggcgcg ggtgcggacc cacaacacca ggtcggcgaa
    28321 ggagaggtcg gagagcaact gccactcgcc gagcagcacg tggatgcgct cgatctgcga
    28381 ttcggtcagg gtggtctctc ggttcaactg ctcggcgagg acgctggcca cgaaacgtca
    28441 gcctacccac cggcgcggca gcgccggccc gcggcggtga ccgccgcggg ccggcacctg
    28501 ccggtcgcga gtcagccctc cgaggcgccc gcccggacca cgctgcgcag cgtgcgcagg
    28561 gcgacggaca gggcggcgat cgtcggctgg tcgaggcgat cgatgccggc gagcgagcgc
    28621 tgggcgcgct gcactgtctc gctgtgcgct gtcagccagc cgtcgagcag cttgtccgga
    28681 tcctgctcgc cggccggtgc cccgtcgagc acggcccggg tgatcgcgtc gaggatgccg
    28741 tagaggtcgt cgcgcagcgc gccgcgggcc agggcctccc agtggtcctc ccgctgcaac
    28801 tgggtgatct tgccgagcag cttgtcgacg ccgagtcgtt cggagttgag gtagtagagc
    28861 ggtgccacga cggcggggtc gaggtccttg tcgatgcaga tgtcggcgat gtcgagcagt
    28921 gagaaggcgt cgagcaggct cgcgcagtcg cgggccaggt cctccggcac cccctccgcg
    28981 acccgggcgg cggtcgcctc ctcgagccgc tcgcgttcgc tgccgacgag caactcgccg
    29041 aggcgggggc gccaggcgcc gatgatcggc tggtagcgct cgatctcgga gccgacgtcg
    29101 agagtcgacg gccgcgcggt gatgaaccag cggaccgcgc ggtccagcag ccgccgcacc
    29161 tcgagataga gctcggtctg ctgctgcgtc gagacgacat tgtcgagcgc ctcgacccgg
    29221 tcgagatagt ccgcgacgcc gaagatctcc ttggcgatga cataggcccg ggcgatctgt
    29281 tcgccgctgg cgccggtctc ctcggtcgcc cggtaggcga aggtgatccc gccgcggttg
    29341 acgatatcgt tggcgacgcc ggtgacgatg atgtcgcggc gcagcggatg cgcggccacg
    29401 gcctccggga agcgctcggg cagcgccggt gggaaataac cccgcagttc cgactcgagc
    29461 caggggtcgt cggggaatcc cgaggccagc aggtcacgct tgagcgtcag cttcgaatag
    29521 gcgaccagca cgcagaactc gggcgagacc agcccggtgc cggccgcgag ccggtcgccg
    29581 agttcggttt cgctgggcag gaactccagc tcgcgattca ggccgccgtg ttcctccaga
    29641 tagcggatga agcgcatgtg caccggcagc atgatccgct gctgggctcg agcgttgccg
    29701 agcaggacgt tctgctcgta gttgtcgcgc aggacgtccg ctgcgacctc gtcggtcatc
    29761 gagcgcagca attcgtcgcg ggccggcagg tcgagggcgc cgtcgcgcat gacctggccg
    29821 agcaggatct tgatgttgac ctcgtggtcg gaggtggcga caccggctga gttgtcgatc
    29881 gcatcggtgt tcaggcggac gccgctgcgg gccgcctcga tccggccgcg ttgcgagaga
    29941 ccgagattgc cgccttcgcc gacgacgtgg caacgcagct ggttgccgtc gacgcggatg
    30001 gcgtcgttgg cccggtcacc gatctcgccg tgggtttcgt cgctggcctt gacataggtg
    30061 ccgatgccgc cgttccacag caggtcgacc ggcgcgagca gcgcagcgtg gatcagctcg
    30121 gcgggggtca tggacgtgac ccccgagtcg atcccgagca cctcggccac ctgcggggtg
    30181 accggcaccg acttcgccga ccgcgggtag accccgccgc cctcgctgag caagcccttg
    30241 tcgtagtcgt cccacgagga gccgggcagc tcgaacatcc gccgccgttc ggcatacgac
    30301 gcggcggcgt cgggggctgg gtcgaggaag atgtgccggt ggtcgaaggc ggcgacgagg
    30361 cggatgtgtt ccgagagcag catgccgttg ccgaagacgt caccgctcat gtcgccgacg
    30421 ccgacgacgg tgaagtcctc gctctgggtg tcgatgccca tctcgcggaa gtgccgcttg
    30481 accgactccc aggccccgcg ggccgtgatg cccatcgcct tgtggtcata gccggccgag
    30541 ccgcccgagg cgaacgcgtc gtcgagccag aagccgtagg actgcgccac gccgttggcg
    30601 atatcggaga aggtcgccgt gcccttgtcc gccgcgacga cgagataggg gtcgtcgccg
    30661 tcgtgacgca cgacccgctc cggtggcacg atgtcggtgc cgacgcggtt atcgctgagg
    30721 tcgagcaggc cggagaggaa gacccggtat gccgccttgc cctcctcgag ccaggcgccc
    30781 cggtcgacgg cggggtcggg cagctgcttg gcatagaagc cgcccttgga gccggtcggc
    30841 acgatgacgg cgttcttgac catttgtgcc ttgaccagac ccaggatctc ggtgcggaag
    30901 tcctcgcgcc gatcgctcca gcgcaggccc ccgcgggcga ccggcccgaa gcgcaggtgc
    30961 acaccctcga cccggggcga gtagacccag atctcgaacg ccggccgcgg ctgcggcaga
    31021 tcgggcacct tggtcgggtc gagcttgatc gagaagtagt ccttgccttc accgtccggg
    31081 ccgagctgga agtggttggt gcgcaaggcg gctcggatga cgccgaggca cgaacgcagg
    31141 atccggtcct catcgagcga gctgacgtcg tcgagggcgg cctggatccg ggcggcaatc
    31201 tcctcctcgg cctcctcacg cccctcgtgc gcacggtccg ggtcgaagcg ggcctcgaag
    31261 agcgcgacga ggtcggcggc gatgtccttg ttgtgcacca gcgccgagac gatgtagttc
    31321 tcggagaagc ggctctgggt ctggcggaca tacttggtga tcgcgcgcag gatgacgacc
    31381 tggcgccatg tcagcttggc ccgcagcacc agcgccccga gcgggtcgga ttcggcccgg
    31441 ttgttccaca ccgcatcgat ggcgccctcg aagagttcgc gcagttcggc gtgtgacatc
    31501 gagtcccaca ggtcgtcggc gggggcgcgc agcccgaagt cgtagacgta catgggggtg
    31561 ccgtcggccc gggtcagctc gtagggccgt tcgtcgacga cctcgacgcc catgtgcgtg
    31621 aacagcggca ggacacgggt gagggagatc ggctcgcggc ggaagagctt gaaccggcgg
    31681 atcgcctcat cgcccgatcg tggtttgtag agggcgaatt cggtgtcggt cggctgctcc
    31741 agcagctcca gccgccgcag gtccgccagg ccctgggcga cttcgaagtc gtccttgtag
    31801 gagatggaga atgccgggcc gtatgccgcg acgagaggtc cggcggcctc ctcaccgacc
    31861 tcgcggatgg cggcgtcgcg gaagtcctcg tcccaggtgc ggatcgccgc gccgatcttg
    31921 tcgttgagca ggtcgatgtc gatgtccggc atctcggcgc ccggctcgag gcgagcgaca
    31981 tagtgcaccc gcgcgagcgg tgactccgac acccgtgccg tgtagtcgat gctcttggcg
    32041 tgcagggctt ctcgcagaat gccttcgacc cgcagccgca cggccgtcgt gtaacgatcg
    32101 cgcgggatgt agaccaacgc cgagacgaag cggccgtagt cgtcgggccg caggaagacg
    32161 cgggcgcgcc gcttctgggc gagcgtcagc accgaggtcc cgacgtcgac gagctggtcg
    32221 acatcggcct ggaagagttc gtcgcgcggg tagtcctcga gaatcccgag caggtccttc
    32281 gacgagtgcg actcgtcttg gaagccggcc ttcctcatga ccgcggcgac cttctcgcgc
    32341 aggtagggca cccggccgac cgactccgcg taggcgctgg cggtgaacag cccgaggatg
    32401 cggcgctccc cggtgatctg gccgtcggga ccgaaggtct tgacgccgac atagtcgagg
    32461 tggacgggcc ggtgcacgct tgagcgggag ttggccttgg tgacgaacag cacccggcgt
    32521 tcggtggcct tggccagcac cgcgtcggac aatgccggcc tgggcacacc catgggcgga
    32581 tcggcccgga gcagccctaa accacttccg gacaccggga tcaggccggt gccgccctcg
    32641 gtcggcgcca gccggtagtc gcggcaggcc aggaacgtga agtggttgtc ggcgagccag
    32701 tcgagcagtt cgaccgcctg cttgacctcg gcctcgggaa tgtcggcggg gacgacggca
    32761 ggcaactcgg ccgcgatcat ccgcgcccgg gcgcgcatct tgggccagtc ctcgaccgcc
    32821 tcacgcacgt cggagagcac cccacgcagc cgggcgacga tctcgtcgcg gtcgggagcg
    32881 tcgctctcgc ggtcgatctc gaggtgcatc cacgactcgt tgagcacccc gacggactgg
    32941 ctctgtccct cgctcggaac ggcgtcgtca ggcaggatcc gcacgagcgc accggtcgcg
    33001 tcccgctcga cccgcaggat cgggtggacc accgtgtgga tgccgcgccc catcgaaagc
    33061 agttgggcgc gaaccgaatc gacgaggaaa ggcatgtcgt cggtgacgat ctggatcttg
    33121 gtgtggctgg ggccgtcacc cagtggcggg gtcgtgcacg cgaccttcgc ggtgcccgcc
    33181 ggccgctgcg tcccgagctc gcggtggctg cggacgatgt cgaggagatc ctgcggagag
    33241 cgttcgagga gatcttcggt gggtgcatgc cggaagtagg cctcgatgag ctcgccctcg
    33301 ccaggtggac cctgctcctc ggacgcaagc tcacatgcct gcgcgatccg gtcgcttcgc
    33361 tgctggattt cctcggatgt ggctgtcaca gagaaattct ttcgtcgatc gcggggggcg
    33421 gcgctgccct ctccgactgt atcgagtctc gtcccacgga tgccacgtgg ctccccggtt
    33481 ccggcccgat ccgcgtcggc tcagcgcaac ccgccggcca gggcctcggc gtcggcggcc
    33541 gcggcggaca ggcgggcgat cagctgctcc cgctcgcgca ccatgtgctg ctccagctgc
    33601 gcggcccggt cgcggacgcc ggcgaggtgt gccgagcgag tagcgctgtc ggccgggtcg
    33661 gcgaccccga cgatcccggg ccgcatcgcg tatccggtcg gaccggtgac cagaccggca
    33721 gccgccgcct cgtcggcgag cgccgcgctc tggtgtccca gccgggcctg ggagtatgcc
    33781 gcgcggttga gttcgtcgcc gccggtgcgc gtcacggccg cggcggcgcg gcatacgtcg
    33841 gcaagctcgg cgagcaggcg ccggttcgcc ggggagcggc gcggggcccc cggggcgggc
    33901 cggctgtcca gggagtccgc ggcgtcgacc agatcacgcg cgacccgctg caatgcggtc
    33961 gccgtggcgg ccagcgaggc gggatcgccg gcgccgttca cggcataccc cacggcggag
    34021 ccgcggcgac gtgcggcgcc gcctggctca ccggcgcggt cggcagccgg ccggcgccct
    34081 cgcgggccgc agcggcgagc atccgcagca gatccatcac ggcgtcgagg tagccgtcga
    34141 cggccgcctg cgcgagccgg tcaccggtcg acggcagcgc cagtagcaac gactcgaccg
    34201 tcgcatcgca gcgctcggcg agcaggccaa gggtcagggc cgggtccggg ggcgctggca
    34261 tacgaccacg ttaccggcca ccggcgccgg ccccgcgagg ttatccacag cctcccggcg
    34321 gggtcgtcag cgctcggcgg ccagccagtc gttgatgatc tccgcctcca ggtcgaatcc
    34381 gtgggagatc gccggggcgg cggccttctc gatcttgccg ccgatgaagg ggaccttggc
    34441 cttcaggtcg gcctccaggg cctggtggct gccgtccgcg gtcggataga cctcgagggt
    34501 gccggcgagc gagatcgggg caccgtgcac cgtgatcgtg acctgggctc ggcggctgcc
    34561 gtccgcggcc ggcgggcccc agtcctggac ctcgtcgacg atgagttcgt cgcccaccat
    34621 agagcgggcc gcgtcgggca ggttgtgcgt gggcatggcc cgtgtcgtgc gcaggatcgt
    34681 ccgctcgccc tgttcgtcga cggcgacggt gtgctcgcgg gggctggtgc gcaggcactt
    34741 ctcgtcctgg aaggcctcgg tggcccgcag ggcgaatacc tcgtctggtg tccccgcgag
    34801 atccgcggac tcggtgatcc tcatggcgga cgacactatc ggtccaggcg ccgcgatcga
    34861 cgcggcgcgt ccgtcccgct gctgtcacgc catgtgcggg tacccgtgcg ccgtcggggg
    34921 cacgaaggtc tccttgatgg cgcgcggaga ggtccaacgc atgaggttga tcatcgagcc
    34981 cgccttgtcg ttggtgccgg aggcgcgcga gccgccgaac ggctgctgcc cgacgacggc
    35041 gccggtgggc ttgtcgttga tatagaagtt gcccgccgcg aaccgcaggg cggcgctcgc
    35101 ctcggcgatg acccgccggt cctgggcgat gatcgagccg gtcagggcat agggcgcgac
    35161 cgactccatc tgcgccatga cggaccggaa tcggctgtcc gggtagacgt gcacggccag
    35221 gatgggcccg aagtactcgg tcgtgaagat ctcgtcgacc gggtcgtcgg cgacgagcac
    35281 ggtggggcgc acgaagtagc cgtcggtgtc gtcataggtg ccgccggcgt ggacggtgat
    35341 gcccgccgtg gccttggccc ggtcgatggc ggcggcgttg cgggcgaagg agcgctcgtc
    35401 gatgaccgca cccatgaagt tgcgcaggtc cccgacgtcg ccttggctca ggccgttggt
    35461 cagctcgacg aggtcgtcct tgatgaccct ccacagcgag cgcgggacgt aggcccgcga
    35521 agccgccgag cacttctgcc cctggtactc gaaggcgccg cggatcatgg cggtccgcaa
    35581 gacatccggg tcggcgctgg gatgggccag gatgaagtcc ttgccgccgg tctcgccgac
    35641 cagtcgcgga taggtgtgat agcccgacag attggccccc acctgctgcc acaggtgctg
    35701 gaaggtcgcc gtggatccgg tgaagtggat acccgccagc cccgggtcgg tcagggcgac
    35761 cgtggagacg gcgatgccgt cgccggtgac catattgatg acaccgggtg gcaggccggc
    35821 cgcctggagc aactccatcg tgtagctcgc ggcgagttgc tgggtcgggc tcggcttcca
    35881 cagcacggtg ttgcccatca gcgccggggc ggtcggcagg ttgccggcga tggcggtgaa
    35941 gttgaacggg gtgatcgcgt agacgaagcc ctcgagcggg cggtgatcga gccggttcca
    36001 gacaccgggc gcattgacca tcggctgctc ctgcaggatc tgccggccga agtggacgtt
    36061 gaaccgccag aagtcgatga gttcacaggc gctgtcgatc tcggcctggt atgcggtctt
    36121 gctctgcccc agcatcgtcg ccgcgttgag cgtggcgcgc cacggcccgg cgaggagatc
    36181 ggcggccttg aggaagatgg cggcccggtc gtcatacgac aactcccgcc atccgggggc
    36241 ggcggcccgt gcggcggcga tggcggcgcc cgcttccttg gctccgccgt tgcgcatccg
    36301 gccgacgatc ttggcgtggg cgtggggttg ccgcacgggg atgcgggggc ccgagccggc
    36361 cacctcggca ccgtcgatca cgtgcggcag gtcgcgggtc tcacccccga tgcgggccag
    36421 ctcggtgagc aacgcctcgc gttcggagga gccgggggcg tagtcgcgca ctggctcgtt
    36481 gaccgggatc ggcaccgtgg tgacggcgtc catttcgtct ccttcgaccg gggtggtggc
    36541 gtcgccaaac agcctagtca ccagccggtg acggtggccg cgagcgctgc cggccgtcga
    36601 tcctcgtcag gagtcggtga ggtgctgcgc gaccaggccg gcctcggtga tgtcgaacca
    36661 gagcaggtcg gcgtcttcgc cgggctcctc gccgacgtgg aaggcgacga tctcgcggcg
    36721 caacaccgct cggtgcacct cgacggtatg gccggtgtcg ggccagtcga cctccgcgtc
    36781 gggcaggtcg agggcggcca cggcgacgcg gtctccggcc gccgcgccgg ccgccaccgc
    36841 gtcgaggaag gccgcgtatt cgcgctcctc ctcgtccgcg cgcggagtag ccgcgaccag
    36901 ggccggcgag acgccgtatg ccgtgcgcac cgggccgggc agctcgccgg aggcgatcag
    36961 cgcggtgacg tcggcggcgg tcacggggat gtagagacgc cgtaacgcca tgtcaattcc
    37021 cttctgtccc aaagggatcg gcgacccagt gcaggagttc ctcgaccgcc tcggcgatcg
    37081 cggcacgcag ctggtccagg tcggggatcg cttcccggtc ggcgagcacg ccgtagtgca
    37141 tggcgccgag atagctcgtc atcccgatcg ccatcgcgtg ccccttggtc agcggcagca
    37201 ccggatagct ggcgacgagg ggtagcccgt cggcatacat ttggcgttgc ggcccaggga
    37261 cattggtgat cacgaggtcg aacagccggg tggacaggga tgcgccgacg cgcaccccca
    37321 gtgagtgcag tgcgggcggc gcgaagcccg ccatccccat caggtccgcg gcgcgcacac
    37381 cggagtgctg cgcgagctga tggtcgaggg cgaactggat ctggcgcagg cgcatcgccg
    37441 gcgaggattc gccgacgggc aggtcgatga agagggcctc gaccgtcgaa ccgtatggtg
    37501 tgccgtcccg cgccaccgag accggcacca tggcccgcac cgatcgttgg gcggggacgt
    37561 gttcaccccg gctctgcagc cagatccgca agccgccggt caccaccgtg agggcgaccg
    37621 cgtgcaggct cggccgtttg tgcccgcccg tggacatggc cttgcggacc tcgcgcaggt
    37681 cggcgagcga gccgtccacc atcaggtagc gccgggcgcc ggcactctcg accgcgaacg
    37741 tccgccccga ggccggacgc gcggccgcct tggccacggt gccggcgacg cgaccgaccc
    37801 ggccggccgt cgcgcgcagc tccgccaagc cgccgcggac ggtgccgacc acgctgccgg
    37861 gtcgcttgac gaggctgatt gcggtgtcgg acaacagttc tcgggcgcta ggaccgggcg
    37921 ttggcgtcca gcccgcgccc ggggcgtcct gcgcacgggc ttcggaggac aggccggtga
    37981 tgtcaccgga ctcgatgagg gcctggtgga ccttggagat gacggcgatg cgtccgtcgt
    38041 cgagcccttc gacgacatat acctcccaca gcgggcgatg ccggtcgagg gggcgggcgc
    38101 acacgcgggc aacgaaatcc tgaagctggg cgcggtcccc cggcgcaggc agggccgagc
    38161 ggcgcacgtg gtagtcgagg tcgaattcgg tgtcgtcgac ccagacgggc gagaccaccc
    38221 gcccgggcag ggagtgcacc cgctggcggt agcggggcat ccgctcgacg cgcgcgctga
    38281 tggccatgga cagcgcgtgt gtgtcatagt ccgcgggcgc atcgaagatg agcacctgcc
    38341 cgacgtgggt cggggtgtgg gcttcctcga gatagaggaa gctgctgtcg gcggggctca
    38401 aacggtcgat cacggggcca tcgtggcaga tcaccgcgtg gggcgcggcc ccgtgcagcc
    38461 ccgcgatccg catagggtgc ttccggaggt gtctgggctc ctggtggtcc ccccggtctt
    38521 caaaaccggt gtgcggcggt atccgtcgca ggcgggttcg attcccgtcc acctccgcca
    38581 cagcgatatc gagcggaggc ggcaggtgac tgggcagcga cacggggacc cgcgccgcgg
    38641 cataccgcgc accgatacgc tgctggccga tccgcagatc gcgtcgtatg ccgccgccct
    38701 cggcgccccg cgggtgcgcg cggtgatcga cgcggtgacg gccgccgcgc gagccggggc
    38761 ggtggcaccc gatgccgtcg ccgcggaggt gcgcgcgcgg ctggcacggt gggcggccag
    38821 cgcgacgacg ctggtgccgg tgctgaacgc gacgggcgtt gtcgtgcata ccaatctggg
    38881 gcgtgcgccg ctgacgccgg ccgcacgcga ggccctggtg gcggccgcgg gctatgtcga
    38941 tgtcgagttc tcgctcgaga gcgggctgcg ggccggccgc gggcgcggca cgctggccgc
    39001 actgcgggag cgggtgccgg gcgcgcaggc ggcgctcgtg gtcaacaacg gcgcggcggc
    39061 gctctccttg gcggtcacga cgtgcgctca gggtcgcgag ctgatcgtca gccgcggcga
    39121 gctggtcgag atcggcgacg gcttccggct gcccgacctg atccgctcga cgggcgtggt
    39181 catccgggag gtggggacga ccaatcgcac ccacccgggc gactacgagg aggcgatcgg
    39241 cgcgcagacg ggagccgtgt tgaaggtgca cccgagcaat ttcgtcgtcc ggggattcac
    39301 ggccgaggtg tcggtgagtg cggcgtcgcg gatcgcccgg gcgcacggcg taccgctcat
    39361 cgtggacacc ggatccggtc tgctggaacc cgatccgttg ctgcccgatg agccggatgc
    39421 ggcgacggcg ctggccgagg gcgcggacct ggtggtgtgc agcggggaca agatgctcgg
    39481 tgggccgcag gcggggatcg tcctgggccg ggccgatctg gtcgaggcga tgcgccggca
    39541 tccgttggcg cgggccttcc gggcggacaa gttgacgcac gccgccctgg aggcgacctt
    39601 gcgtgggccg cgcggtctcg tgtccgacta tctccacgcg cccacggagg agctgcgggg
    39661 ccggaccgag gccctcgcgc agcggctgcg ggcggcgcgg ccggacctga tgacggacag
    39721 tcgtgtggtg gccgtggccg gcgcggtggg cggtggcggc ggcgcggagg tgccgctgcc
    39781 gggctgggcg ctggcactcc ccgaggcgct ggcgctggtg ctgcgcgccg ggcggccggc
    39841 cgtcgtggcg cgacaagagg gcggccgcac gctggtggat ctgcggtgca ttccggtcgc
    39901 tgacgaggcc gacttggccg aggccctgct tgctgggctg gaacgccttg cggcagaggg
    39961 caactgatgc acgtcatcgc gacggccggg catgtcgatc acggcaagtc gacgctggtg
    40021 cgggcgctga ccgggatcga gcccgaccgg tgggaggcgg agcggcgacg cgggctgacg
    40081 atcgatctgg ggtatgcctg gacgacgctg ccgggtgccg gggaggtggc gttcgtcgac
    40141 gtgccggggc atcgccggtt catcggcaat atgctcaccg gcctgggtcc cgcgccggcg
    40201 gtgcttgtcg tcgtcgccgc ggatgccgga tggtcggcgc agagcgagga gcatctgcgg
    40261 gcgatcgacg cgctggggct gacgcacggg ctcctggtcg tcacgcgggc cgacctcgcc
    40321 gaccccgcca tggccgctcg cgtgggcgct gcggcgcgcg agcggatctc ggcttcctct
    40381 ctgggagagg tggattcggt gatcgtgtcg gccgtgtccg ggcaaggcct cgatgacgtg
    40441 cgggacgcct tggggcggct gtgtgccacc cttccggctc cggtgcgtga cgggcgggtg
    40501 cggctgtggg tggaccgggc gttcacgatc cccggcagcg gcaccgtcgt caccggcacc
    40561 ctcggcgcgg gcaccattgc ggtcggggac gtgctggagg tggccggtcg gagcgagacc
    40621 cggcggcggg tgaccgtgcg cgggctgcag tccctggaac gctcccggga ggcggtcacg
    40681 gcggtggcgc gggtcgcggt caacctgcgc ggcgtggccg ccggcgaggt gcaccgggga
    40741 gatgccctgc tcaccccgga gggttggcgg tcgacggcga atgtcgacgt ccgaacggcc
    40801 ggaccggtgg cggatctgcc gcgagaggtg ttgtgccaca ccgggaccgc cgccgtcccc
    40861 gtccgggtcc gtccgctctc ggcaggagcc gcacgcttgt cgttcgagcg gccgctgccc
    40921 ctggcgaacg gtgaccgcct gatcctgcgg gacccgggca ccgagggcgc gctgaccggc
    40981 gcggtggtcg tcgacacggc ccccgcggcc ctgacccgcc gcggcgacgc ggcccgccgc
    41041 ggcgccgaag tcgaggccag cgatcaggtc acgctcgccg acgaagtgcg ccgccgcggc
    41101 cacctctccc gagcggacgc caccctcctc ctcgggcacg accccggccc cccgtcgaga
    41161 gagcactttt cacccgtcga gagtgcacgt cgtggccatc gcgtgaacga cgtggtgggc
    41221 gtcggcgact atttcgccac gcgcgagcgg gtggccgcct ggactcgggc cgtgctggcg
    41281 gtcgtcgacc ggcgggccgc cagcgatccc ctcgatccgc tcgtgccgat ggcggcggcc
    41341 cgggacgcgg ctggagtgcc ggatctcgtt gtgctggagc agctttgcga cgccgcggga
    41401 ctgcaggtgg agcgtggccg ggtatgccgc ccgggcaccc agccgagcct ggggccggcc
    41461 caggccgggc tcgaccatct gctggcgcgg ctgaccgagg atcccttcgc ggcaccggag
    41521 gcacccgatc tggccgcggc cggcctcggg ccgcgtgagc tcgccgccgc ggaagcgttg
    41581 ggccgtctcg tgcggctgcc cgatgacatc gtgctgctcc cgaccgggcc ggcccaggcg
    41641 atgcgcatcc tcgccgccct tgcccagccg ttcacgacaa gccaggcgcg ccaggcgctg
    41701 ggcaccacgc gccgggtggc catccccctg ctggagcatc tcgaccagcg cggctggacc
    41761 cggcgcctgg acgccggcca tcgtgaggtc gtgcgatgag actgggccac aaccgtattc
    41821 accacgtaag gacgacgtca tgactgagac ccggctgacc cagttcgcgg ccggcggggg
    41881 ttgcgcgtgc aagatcccgg cggcccagct ggagggcatc gtcgccggac tgagcggcgc
    41941 gacgcacccc gacctggtcg tcggcctgga caccggcgac gacgccgcgg tggtgcgcat
    42001 cgccggcggc caggccgtca tctccaccgc cgacttcttc accccggtgg tcgactcggc
    42061 ctacgactgg ggacggatcg cggccgccaa tgccctctcg gatgtctatg cgatgggcgg
    42121 taagcccgtg ctggccatca acctgctcgg ctggcccgtc gacgtcatcg acaccggcct
    42181 catccctgac gtgctccgtg gcggcgccga catcgccgag ggggccggca taccgctcgc
    42241 cggggggcac agcatcaccg cgccggagcc catctacggg ctcgcggtca ccggcgtcgc
    42301 ggacccggac cggttgctgc gcaatgacat tgcggagccg ggcttgccga tcagtctgac
    42361 caaaccactc gggctcggga tcctgggcaa tcggcacaag gccaccggcg agatcttccc
    42421 cgaggcgatc gcgacgatga cccagctcaa cgacgccgcg tcggccgccg ccctggcggc
    42481 cggcgtgcgc gccgccaccg acgtcaccgg cttcgggctg ctggggcatc tgttcaagat
    42541 gtgccgcgcc tccggggtct ccgcgcggat cgaccgggcg gccgtgcccg tcctcgaggc
    42601 cgccgtggcg agcctgcgcg agggctatgt gccgggcggg tcgcggcgca acctcgactg
    42661 ggtccgcccc cacctcgacg ccgcccccgg ggtcagcgag gacgacctgc tgctgctcgc
    42721 cgacgcccag acctccggcg gcctgctgat cgtcggcgag atccccggcg cgcccgtcat
    42781 cggcgagacc acacggcata ccggctccgg gccacgcatc acgatcccct gaccggccac
    42841 cccgtcgcca ggagcccttg cgacgcgggc ggcggggcg
//
LOCUS       DAOPJG010000006        42218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_954_length_42218_cov_7.703864, whole genome shotgun sequence.
ACCESSION   DAOPJG010000006 DAOPJG010000000
VERSION     DAOPJG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 42218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42218
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_42218_cov_7.703864"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKU75_01310"
     CDS             complement(<1..662)
                     /locus_tag="PKU75_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPF79541.1"
                     /translation="MSTLPATEPARLVARTIAVPDETAAELLTLIPAQVPAEHVCSWV
                     RRGEGLVGWGRALDFETQGADRFAQAARWWRALCTDAVIRDEVAAPGTGPIAFGSMAY
                     AAHSPQRSRLVVPEVVVGRRAGRAWLTTITAGGQLPPTPHDLNPAPEPPSPGVIAFAD
                     GPKAAAEWASAVGEAVERITSGALDKVVLARSLVARAEAPIDPRFLLRRLADSYDNTW
                     VFA"
     gene            805..1500
                     /locus_tag="PKU75_01315"
     CDS             805..1500
                     /locus_tag="PKU75_01315"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HPF79542.1"
                     /translation="MSRASLEKDPAEVAAMFDDVAAKYDLTNDVLSLGQDRRWRKVVV
                     EAVAAAPGEAVLDIAAGTGTSSEPYADRGVHVVPADFSLGMLQVGKRRRADLAFTAAD
                     ALHLPFADGSFDAVTMSFGLRNVSDPSAALREFARVTKPGGRFVLCEFSAPTNGAFRT
                     VYTEYLMRALPPVARRVSSNPDSYVYLAESIRAWPAQREMAQVIADAGWGELRWRNLS
                     GGIVAVHHAVKPA"
     gene            complement(1487..3169)
                     /gene="menD"
                     /locus_tag="PKU75_01320"
     CDS             complement(1487..3169)
                     /gene="menD"
                     /locus_tag="PKU75_01320"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HPF79543.1"
                     /translation="MPAVDFATHLIDALVGMGVREAVLCPGSRSAPLAYAAYAAEQAG
                     RLRLHVRVDERSAAFLALGLAKTSRVPAAVITTSGTAVANLHPAVLEAHHGKVPLIVL
                     SADRPAALRGSGANQTTIQPGIFPGAVRLEADLDETAAPWDVAGEAWRAATGAAGDPG
                     PVHLNICLSEPLVPAQWPMPPAEPSADTPPQDGENTCPTTDLTLDGDRTLVILGDLVD
                     PRIRPAVVDWAARHSLPVIAEPFGEHGPEPGVIPHGPLLLTDQRFLDSFAPQRVLVAG
                     RLTLSRPVTALLRRPGLRVEALADTSAHADPVGVVNAGHELYAVLAAGLRAGTSAGQS
                     AWADAWRDSGVAIAEAVAADPPPWPSGLAVAATLVATLPERAILFTGPSNPVRDIDFG
                     VAQAPAGSTIVASRGLAGIDGCVSTAVGIALCAPGRPAYALLGDLTFLHDSNGLLIGP
                     DEPRPDLTIVVVNDAGGGIFGLLEPGRPPLRKPFERIFGTATGTDLGTLARAHGIPHA
                     LVAGPEELTAAIAEPPHGIRIVEVRVERDSHAAAHAHLREVAAAALRDVRPA"
     gene            complement(3199..3933)
                     /locus_tag="PKU75_01325"
     CDS             complement(3199..3933)
                     /locus_tag="PKU75_01325"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF79544.1"
                     /translation="MDVRWMWIFVDALAPAGPAWDFWQAATRSARSATRGDHDEFATL
                     LPAQGDPWVKVQEVRSGGGIHLDLDVPDPLAAAEDVVAAGAGEVFRYADGGCVILRSP
                     GGFVFCLTRWDGAAARARAGEPDLLDQLCLDIPAASYEVECAFWGRLLGADPVRGSLP
                     EFRAVPRASHLPVRILLQRKDSGDGPVTGHVDFACADRQATRRAHEGLGAAYRGSGPH
                     WTVMRAPGGQTYCLTDRDPETGTLRA"
     gene            complement(3981..>4494)
                     /locus_tag="PKU75_01330"
     CDS             complement(3981..>4494)
                     /locus_tag="PKU75_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPF79545.1"
                     /translation="ARWVEGVLDVHYGIATSPVSLRSLIGAWIEQAPLAAPAAAVALL
                     RRLVEAAPDDAGPRHLVHGDHRAANILVRDGDVVAVLDFEEARIDHPIAELARSTVLL
                     GTRFHDWGPMGPPTREAFRAGYEAVRPLTAAESAWWRPLILWWSLLFVPEGEDPTGWR
                     AAVLAQIDDR"
     assembly_gap    4495..4594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4595..4853
                     /locus_tag="PKU75_01335"
     CDS             <4595..4853
                     /locus_tag="PKU75_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79546.1"
                     /translation="RTPAEDKRQRTVSLTAAGAAVFRQAKREIRRVESALLEQAFTPA
                     EAASLRSQLERLAEACSGRPSRVHSERSDRKPGGGGGGGAA"
     gene            4863..5234
                     /locus_tag="PKU75_01340"
     CDS             4863..5234
                     /locus_tag="PKU75_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HPF79547.1"
                     /translation="MSETTILHNPRCSTSRAALEKADALGSDVAIRDYLKEPLTEQEL
                     RDLVAILQDPVEDLVRRDPHFLELGLDVEHVDDAESVIATLLAHPKLLQRPILIRDGV
                     AIIGRPKERVAAFLKGDVHAE"
     gene            5231..6352
                     /gene="menE"
                     /locus_tag="PKU75_01345"
     CDS             5231..6352
                     /gene="menE"
                     /locus_tag="PKU75_01345"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HPF79548.1"
                     /translation="MIELVDASDPPALLPRLRRALEGDAPLHPYAGTPLPLPAHDAAD
                     LPADLALVVGTSGSTGEPKLALHGAAGLLASANATYAVLDGPGQWVLAIPPHHIAGLQ
                     VLIRSLVAGTTPVVMDRTRGFTPAAFAQATQRLGEGTAYTSLVPTQVTRLLADKVGTA
                     ALQRFSAVLVGGAAVPLALREQTGATGVRLVATYGMSESAGGCVYDGRPLPGVEVAVD
                     ESGRIHLGGATIAHGYLGRPAEAFTRRGDVRWFATDDLGELTADGELRVHGRIDDVIT
                     TGGMKVHPRVVEEAALAAYPHLRAAVAVGIPDPEWGQLVALALVTDTPVTTDEARARL
                     RGLLPAYALPRLVRTSATPLPERGPGKPDRRAIAVWVGQ"
     gene            6357..6623
                     /locus_tag="PKU75_01350"
     CDS             6357..6623
                     /locus_tag="PKU75_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HPF79549.1"
                     /translation="MIRFLPGLLALVLAIYTLVDCVQTPEEAVRGLPKVVWVFVILLF
                     PFAGPAGWWFAGRPKTYALPGRANPNQPPRPKGPDDDPDFLRGL"
     gene            6663..7538
                     /locus_tag="PKU75_01355"
     CDS             6663..7538
                     /locus_tag="PKU75_01355"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HPF79550.1"
                     /translation="MATPAQWIAGARPRTLPAAVSPVLVGTGAAAAIDRADLGLALLA
                     LVVSLALQVGVNYSNDYSDGIRGTDHERVGPIRLVGQRLATAKNVKLMAFLFFGLAGL
                     AGLALCALSDTLGLIWVGVLCVLAAWFYTGGKNPYGYRGLGEVFVFVFFGLVATLGTL
                     YTQAHRLTAPGLLGAYGVGALATAILVANNLRDRPTDAKVGKRTLAVKLGDARTRALF
                     VFLTATAVAAGAACALWAPWALLCLGALALAIPQVMRVQAGATGRDLIPVLSGTGVTQ
                     LLYAVLLTVGLWLGR"
     assembly_gap    7571..7670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7816..8574)
                     /locus_tag="PKU75_01360"
     CDS             complement(7816..8574)
                     /locus_tag="PKU75_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF79551.1"
                     /translation="MDPFALTGKVALVTGGARGIGAASAALLARCGADIAIVDVLDTS
                     AAVAAVVAEGRRGVGYAADLRSEIAVGELVARVVADFGGLDIVHNNAGISRCSAAEDT
                     TFEQWREVLAIDLDAVFLVARAAGRALIAQGRGGSIVNTASMSGLIVNYPQEQVAYNA
                     AKAGVIQLTRSLACEWAEFGIRVNAVSPGYIATDMTPGTSPEGWYEAWLERSPTKRLG
                     TPEEVAGAVAYLASDLAGFTTGANLVVDGGYSCY"
     gene            complement(8582..8857)
                     /locus_tag="PKU75_01365"
     CDS             complement(8582..8857)
                     /locus_tag="PKU75_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HPF79552.1"
                     /translation="MVRYSLMRFLIFFGCLGLLWLLGLRERNEQPWLVVGAALLSMLI
                     SYFALRPLRQDTVDKLSHRVESRVASKRADSDEAVEDSLDGGANDYR"
     gene            complement(8864..9859)
                     /gene="ccsB"
                     /locus_tag="PKU75_01370"
     CDS             complement(8864..9859)
                     /gene="ccsB"
                     /locus_tag="PKU75_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPF79553.1"
                     /translation="MTRETLEQYSLISLYSAIVVLTLAMLGFVLYLSRSAAARAADAV
                     VPEPVAQPVTVGGGGGALTAEPGGTDIPASRDLSRARKAAGPARMFTWLATFLVGCSI
                     VLRGMSVHRPPLGNLHEFVAAASFVVLLGYCLWTLKRNVDWLGIFVTTPVLLMLGSAT
                     TFWYTEAAELMPSLKSGWLVIHVTVATLATGVFTLAAMMSLAYLLIDRAERNPDASQG
                     FVGRLRRIVPPASDLERIGYGLHVLAFPLWTFTLIAGAIWARQAWASYWNWDPKEVWT
                     FIIWVIYAAYLHARATTGVTRQKATYIALAGFACIIINYSIVNMYFVGQHSYSGV"
     gene            complement(9876..11441)
                     /locus_tag="PKU75_01375"
     CDS             complement(9876..11441)
                     /locus_tag="PKU75_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPF79554.1"
                     /translation="MTQTPEAITTKPDPVTQPTLGPLGWVRWAWRNLTSMRTALQLLL
                     LLAVAALPGSIWPQRSINATRTADYIAAHPTMGPVLDKLGAFEVYATPWFSAIYLLLL
                     VSLLGCVLPRTRIHLRHVRGRPPRVPAHLSRLAAYSSREVDADPAAAVADLRAALGRR
                     YRVDVRDGHTLAAETGFAKESGNLLFHLALIGIIVGVAIGHVFGWKGDVIVPVGSSFS
                     NTAINYDTISPGPRVDTAELPPFSLTVDRFDAVFEDRVRGDAQFGQPRDFTAYLRTVA
                     EPGAAEKAQVLKVNHPIEMDGATIFLLGNGYAANVTVKDSRGEVVYKGATVFLPRDNN
                     YTSSGAIKVPGAQPQELGFFGNLLPTAVITKDQGPISVFPDAREPAMALGLYEGELNP
                     GGASGSVYTLNTKEMTVVDDANGKPLRIWITPGETVQLPDGRGSITFESVVRWAGLSI
                     RHDPAKDLTLWSSLLALAGLLASLLVRRRRVFARVTPLAAGGSRVEIGALSKGDDPGL
                     QEHLDRLAARIAP"
     gene            complement(11438..12211)
                     /locus_tag="PKU75_01380"
     CDS             complement(11438..12211)
                     /locus_tag="PKU75_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPF79555.1"
                     /translation="MAGQLIASSASETVASGALPLALLVAALAGLVSFASPCVLPLVP
                     GFLGYVAGAGPEVAPGERGEPTRSRVVAGTLLFILGFSVVFVLITIFVTGIGRAFVEH
                     QDALRRIGGGLVILMALVFLGAGAQRTFTPSWRPRMGLLGAPALGAVFGLGWTPCSGP
                     TLGAVLAMATATDPSMGRALSLVAAYCLGLGLPFLAIATAWERAGRFNAFLRRHSRAI
                     HIGGGLLLLLVGVLLLTGAWTSIMTWLQIHAINGFEVIL"
     gene            complement(12211..12801)
                     /locus_tag="PKU75_01385"
     CDS             complement(12211..12801)
                     /locus_tag="PKU75_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPF79556.1"
                     /translation="MRPLPRRAALLAAAGLALAGCSGNTIADQAKEGSNKGFIAGDGT
                     VEMIAADQRGTPVTLAGTTLDGQAWTSAEHRAKVLVVNVWASWCGPCDKEAPDLVTVS
                     SDPAVTAVAEFVGVNFREEAATGSAQVEDWKLPYPSLSDPGGQAVLALQGKAAAMPST
                     LVLDHDGRIAARILGPVSTSTLTGMIEDVAAEAPAK"
     gene            complement(12798..13466)
                     /locus_tag="PKU75_01390"
     CDS             complement(12798..13466)
                     /locus_tag="PKU75_01390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF79557.1"
                     /translation="MRQSRYEEAEPVTERTLVHLVRHGEVHNPGKVLYGRLDGFHLSE
                     RGHAMAQVVADHLAGRDITYLVSSPLTRAQETIAPLAERLGLPVTLDDRVVEAANSFE
                     GEQVSLGNFLRPRNLIRMYDVTKPSWGEPYVEIVERMTAAVEAAREAAIGHEAVIVSH
                     QLPVWMMRAAVEGRRLWHDPRSRLCSLASVTSIGFHGTEIASLSYAEPAKHLIDAESA
                     SVGA"
     gene            complement(13447..14790)
                     /gene="hemL"
                     /locus_tag="PKU75_01395"
     CDS             complement(13447..14790)
                     /gene="hemL"
                     /locus_tag="PKU75_01395"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPF79558.1"
                     /translation="MARMPSAPDSAPASEALFARARSVVPGGVNSPVRAFRSVGGNPR
                     FVTSARGPYLTDADGREYVDLIGSWGPMILGHTHPDVLAAVTEAAGRGFSYGTPSENE
                     VALAEEIVARVEPVEQVRLVSSGTEATMSALRLARGITGRSRVVKFAGCYHGHVDALL
                     AAAGSGVATFGLPDSAGVPASAAAETIVLPYNDVAALEAAFDTDGEHIAAVITEAAPG
                     NMGVVPPLPGFTEALRRITREHGALLISDEVMTGFRCSRAGWYGLEGPYAAGAPDLFT
                     FGKVMGGGFPAAAFGGPAGLMAALAPDGPVYQAGTLSGNPVATAAGLATLRGCADAVY
                     AHLDHAAKTIADAAVSALTGAGVPHTIQWAGSMFSIFLTDRTVTNYDDAKTTNAPAYA
                     AFFHSMLDQGVHLPPSAYEAWFVSAAHDDAALDRVLAALPAAARAASESFATIAL"
     gene            complement(14816..15310)
                     /locus_tag="PKU75_01400"
     CDS             complement(14816..15310)
                     /locus_tag="PKU75_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPF79559.1"
                     /translation="MATDSTPETRWLTPDEQQAWRAYLRASRLLEVALDRDLAHHGTQ
                     LSEYEVVSMLSEAPAGRMRMSALADLVVQSRSRVTHTAARLEKRGWVRREACVDDRRG
                     VELVLTEQGRAHIDDMARCHVNSVREHLLDVLTPQEFRDLGHSMQKIRDALAEPGQAG
                     DAIR"
     gene            15441..15974
                     /locus_tag="PKU75_01405"
     CDS             15441..15974
                     /locus_tag="PKU75_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPF79560.1"
                     /translation="MPTITDLTPGTWTVDPAHTEVGFVARHLMVTKVRGTFADVEGTV
                     EVAPDVTKSTVDVTVQMASVDTRSADRDTHLKSADFFDVENYPTMRFVSTSFDGERLT
                     GDLTIKDVTKPVTFEVEFNGEQADPWGNTKAGFEAEAEVKRADWGLTWNAAIEGGGVL
                     VSDKIKIVLDVQLLKQA"
     gene            complement(16006..16995)
                     /gene="hemB"
                     /locus_tag="PKU75_01410"
     CDS             complement(16006..16995)
                     /gene="hemB"
                     /locus_tag="PKU75_01410"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPF79561.1"
                     /translation="MTLVPGGGFPAVRPRRLRVSAPMRRLVAETRVHPAQLVLPVFVR
                     EGIGSPQPIEAMPGVVQHTIESLVAEAHRCADAGLGGIMLFGVPVEKDEQGSGADDPD
                     GILNVATRAVVDAVGDRLVVMTDLCLDEFTSHGHCGVLDAAGRVDNDATLQRYAAMGL
                     AQARAGAHVLGLSGMMDGEVGHVRTALDANGFGDVTLLAYTAKYTSGLYGPFREAVAS
                     SLVGDRAAYQQDPANATEALRELAQDVAEGADIVMVKPAGPHLDIVAAVAAHSPVPVA
                     AYQISGEYSSIVAAAERGWIDRDRVMMESLTGIRRAGATVILTYFALEAAALL"
     gene            complement(16992..18620)
                     /locus_tag="PKU75_01415"
     CDS             complement(16992..18620)
                     /locus_tag="PKU75_01415"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPF79562.1"
                     /translation="MSPTDSAPSRTPNAPAAAVAFVGAGPGDPGLLTLAAADALERAD
                     VIITDQLDRADIIRCHVRPDTTIIEASHGSHGVPLTHASRAKLVVQSAQAHPGGLVVR
                     LMDGDPSIFNGLAEEATACRKAGIAVDIIPGVSAASAVPAYAGIPLTAPTTTGIHVIA
                     AGDPGCDFTAGAGASTTVVIFGDAAELGRAFASLLEAGRDPETVVAVTEHGTTTRQRT
                     HTVRLAQAAKIVGGLEFPTLAVLGPTVELRDRLSWFENKPLFGWRVLIPRTKDQAGPA
                     ISLLARHGATAEVVPTISVEPPRTPHQMERAIKGLVTGRYEWVGFTSVNAVKAVRERF
                     EEVGLDARAFAGLKVAAVGGVTADALRAWGIQPDLVPSGEQSARGMLEDWPPYDPDLD
                     PIDRVFLPRADIATDTLVAGLRDLGWEVEDATAYRTVRAAPPAPEIREAIKAGDFDAV
                     VFTSSSTVRNLVGIAGKPHASTVVACIGPATAKTAEEHGLRVDVLAPEASVPSLIDAL
                     AEFGQARAAEAHAHGEAVRRPSERRRAAGRRRAR"
     gene            complement(18617..19603)
                     /gene="hemC"
                     /locus_tag="PKU75_01420"
     CDS             complement(18617..19603)
                     /gene="hemC"
                     /locus_tag="PKU75_01420"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPF79563.1"
                     /translation="MTALRLGTRRSRLALTQSGTVADALRALGHEVETVEIVTEGDRS
                     TAALDQIGGTGVFASALRTALREKQIDFAVHSLKDLPTAAESGLTIAAVPVREDVRDV
                     LVARDGLTLGELPRGARVGTGSPRREAQLHALGLGLDVVPVRGNVETRLRLVESGAVD
                     AVVLARAGLLRLGLSDAATEVLDPIQILPAAGQGALAVECRADDADLRSLLLALEDAD
                     TRACVRAERAVLRELEAGCIAPVGVLAEVVEGFDAAELSIRAFVGARDGSLELRRSLT
                     GDLGDPEGAGIRLARLLIDDGADVVREVRPAPSHRPSESPSTAQTDTPETLQ"
     gene            complement(19600..20934)
                     /locus_tag="PKU75_01425"
     CDS             complement(19600..20934)
                     /locus_tag="PKU75_01425"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPF79564.1"
                     /translation="MSLLVLGLSHHNCPLDLLERISLDSAARRALGHELAGSEHVAEV
                     VVVSTCNRTEVYTEAVTFHGAVSAVTEALGSVTGVERHDLTERLAVHYEDRAIAHAFR
                     VAGGLDSMAVGESQIRAQLRASLHEAQEERVVGPALNGLFQQALRVGKRVHTETGLAN
                     VAGSLVGAGLAVAGAHVGPIEDTRVLVVGAGAMAALAAITARRAGAGRLTIANRTLAR
                     GQALAERAGGDAVRLSELPQALAAADIVIACVGSPGVVITEAMAAEALTHREGRPQAY
                     IDLALPHDVSHAVADLPGAIRVGLEHLAETLHTDGVDPEVAAATDIVTGEVAAFLTAR
                     SAQAAAPTIAALRARATEVLDTELARLAGRTPDVDDATRREVEIAMGRLVDKLLHGPT
                     LRVKELAASGRIEAYADALAELFALEPETVASVSVPPPFEPAADRDPAGGAR"
     gene            complement(20931..21599)
                     /locus_tag="PKU75_01430"
     CDS             complement(20931..21599)
                     /locus_tag="PKU75_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPF79565.1"
                     /translation="MPATDLPRRGIPDATVARLPAYLRVLGIMAEAGVRSTSSLELAQ
                     AVGVHPAKVRKDLSHLGSHGVRGVGYDVAHLTVEISRELGLTRDWPVVIVGLGNLGRA
                     LAGHAGLSSRGFSVAGLFDADPAVIGQSVSGLTISPMSAIADLPRAADTVGVIATPAA
                     AAQVVADQLVAAGITSILNCAPAAIVVPAPVDVRRVDIASELQILAFHKQRRTAAPAL
                     AVAP"
     gene            complement(21725..22000)
                     /locus_tag="PKU75_01435"
     CDS             complement(21725..22000)
                     /locus_tag="PKU75_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPF79566.1"
                     /translation="MAAAAPGPWTPTAPLTLIGKPGCHLCDTVRPVVLALAEEFGVGV
                     EEVSIRDHPDLAAAYGEMIPVTLLDGAVHDYWRIDADRLRAALVRRL"
     gene            complement(22000..23163)
                     /locus_tag="PKU75_01440"
     CDS             complement(22000..23163)
                     /locus_tag="PKU75_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPF79567.1"
                     /translation="MAASKSAASKTTPKAATRAKTAVQPTTSTAPARRRRRASVAAGA
                     ARASGERARHRTTPLLADAPEPPHLRVVGPDESAPQAAAPDLGAIVDAAQGVATTLAS
                     ALGIPREDAERKVAEGLAFLRRRLTGDFTVDAYGFDSDFTDHVLMPLLRPLFYKWFRT
                     EVRGVENIPADGGVLLVANHSGTIAVDSLMVGVAIRDQHPQHRALRELGADMVFSTPG
                     LATIARMGGSTLATNVDAERLLADGELVGVFPEGFKGVGKPFSERYKLQRFGRGGFVS
                     AAIRTGVPIVPCSIVGAEEIYPMLANLKPLARLTGLPYFPVTPLFPHFGLLGAIPLPS
                     KWIIEFGAPVETAHLGAGAADDPMIVFDLTDRIREGIQQSLYGLLTQRRSAFF"
     gene            complement(23165..24214)
                     /locus_tag="PKU75_01445"
     CDS             complement(23165..24214)
                     /locus_tag="PKU75_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPF79568.1"
                     /translation="MADVVLITGVSRYLGGAFARALSQVDPARTIVGVDVIPPPHDIG
                     RAEFVRADIRSPMIGKLIEQYAVDTVVHMGVIATPLGAGGRATQKEINVIGTMQLLAA
                     CQKAPRVRKLVVKSTAGVYGASHRDPALFTEEMAAKRLPKLGFGKDSVDVEAYVRGFA
                     RRRPDVGITTLRLANVIGPRVRTAMTDYFRLPVVPIPLGWDARLQFLHEDDVAAAMIA
                     ATLGEPADTVNVAGDGIITVSQAIALAGRPFVTIPTPLSTGLSTIFKRTGLIDFSPEQ
                     LDLLVYGRGLDTTRMKTVLGFEPAYSTKAAFEDFVAAAPRPVDTALTAIAGAVDDAGS
                     ALHSLVGAVPIGRNR"
     gene            complement(24242..24340)
                     /locus_tag="PKU75_01450"
     CDS             complement(24242..24340)
                     /locus_tag="PKU75_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HPF79569.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLRKTRHQRRNKK"
     gene            complement(24572..24772)
                     /locus_tag="PKU75_01455"
     CDS             complement(24572..24772)
                     /locus_tag="PKU75_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF79570.1"
                     /translation="MTADRQLSEVRFLTVAEVAAIMRVSKMTVYRMVHGGELPAIRVG
                     RSFRVPEDAVDAYLKSSYIETA"
     assembly_gap    25055..25154
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25220..26410)
                     /locus_tag="PKU75_01460"
     CDS             complement(25220..26410)
                     /locus_tag="PKU75_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HPF79571.1"
                     /translation="MVAQARVIWDPGFTAYNFGPGHPMGPLRLDLTARLCEALGVFDA
                     VEVISPPVASDEMLQTVHDPDYIAKVKEASDDPSSAEPAYGLGTEDDPAFTGMHEASA
                     RIVAGTVAMCEQVQAGEIAHGVNFCGGLHHAMRGNASGFCIYNDIAAGIQALLDDGVE
                     RVAYVDVDVHHGDGVEAIFADDPRVLTASIHESGRTLFPGTGWARDVGTGAGEGYAVN
                     VALPPGTTDSAWLRAIRASIVPILRAFRPQFLISQHGCDTHADDPLANLVMTVDAQRQ
                     AMLDIHDLAHNLCDGKWVALGGGGYELVDVVPRSWTHMTAIAAHRPIPVDTPVPEEWQ
                     EYVERTIGRRGPNRMGDLPVGEPAIWVQPWEMGYNPHSPVDRAIMATREATFSHHGLD
                     IWYD"
     gene            26479..27138
                     /locus_tag="PKU75_01465"
     CDS             26479..27138
                     /locus_tag="PKU75_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPF79572.1"
                     /translation="MGKRQHDVLVIGLGRFGGAAALELNRLGHRVTALEKDPVIAESF
                     LGKVFKVVHADASLPHVLAEQKCQNFPVAIVGIGFSVESSVLAAANLVDADVPDVWAK
                     ALSPEHARILQRIGVGHVILPEAETGRRVAHLVGGGLLDYMEFDDGFAMVKMAPPSEA
                     VGFTLAQSAIRSKYGVTIVGVKSPGRDFVHATPETKVTSHDTLIVSGPTALIERLAAR
                     P"
     gene            complement(27244..27756)
                     /locus_tag="PKU75_01470"
     CDS             complement(27244..27756)
                     /locus_tag="PKU75_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79573.1"
                     /translation="MTEIRVRPLTEADWGQFRAIRLAALEESPEAFVAKLADEQEYDE
                     DFWRARMRRAQRMVADVDGSDVGVLSVGVLDDAENTAEIFGLWVRPDWRGRSVAATLV
                     RSASQQAAEDGYDRIAYWVSTDNGRAVAFASSFGFRPTGRRRPMRVTNGADGEEEIAM
                     VLALAEDRAG"
     gene            complement(27811..28608)
                     /gene="proC"
                     /locus_tag="PKU75_01475"
     CDS             complement(27811..28608)
                     /gene="proC"
                     /locus_tag="PKU75_01475"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPF79574.1"
                     /translation="MTIAIFGAGVMGETLLSGLLRSGLAPGEVVITERRADRAAELEA
                     KYGVRALTNAEGAAVADVLVFVVKPQDMTALLAEIGDQIAPGNLVVSLAAGVPTQAIA
                     AGLPEGTPVVRVMPNTPALVDQGMAAFSRGAHVTDEQMERAMSLLRSCGRAIEVPEGY
                     QNAVTAISGSGPAYVFYVVEAMIEAGVVLGLPRETATELTVQTLFGAATMIRETGTHP
                     TVLREQVTSPGGTTAAALRTLDDHKVRAAFVSAIEAAARRSRELAET"
     gene            complement(28622..29572)
                     /locus_tag="PKU75_01480"
     CDS             complement(28622..29572)
                     /locus_tag="PKU75_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPF79575.1"
                     /translation="MGPSELLRKTLLTLAADTRVRDLIERAPVSRTVVRRYVPGAYDT
                     DAVAAVAELRATDRRATIDHLGEDTTDRAQAQAVTQAYVSLIDLLAAEEHTHGGWAEV
                     SVKLSAVGQLLPDEGAAIALENVRIICAAAERAGTTVTVDMEDHTTTDATLAAVRELR
                     RDYPWVGVAVQAYLHRTEADCRDLAAAGSRVRLCKGAYNEPETVAFQDDDEVDKSFVR
                     CLRILMDGAGYPMVASHDPRLIDIATALAESAGRAADSFEFQMLYGIRPEEQRRLADR
                     GHRMRVYIPYGEEWYGYLMRRMAERPTNAMLFVRSLLSRS"
     gene            29677..30414
                     /locus_tag="PKU75_01485"
     CDS             29677..30414
                     /locus_tag="PKU75_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79576.1"
                     /translation="MMNTAAIVIDDLLVRRGGVVVIPGLRVCVPAGQVVGLLGPSGSG
                     KSTLMRAVVGVQKIAGGTVTVLGQVAGSPALRSRVGYVTQSPSVYADLTVRANVAYFA
                     GLVGQGSDAVDKALAEVDLSAVADRKVERLSGGQRSRVSLAAALAGDPELLVLDEPTV
                     GLDPVLRRDLWNLFHRLAGAGRSLLVSSHVMDEATRCDRLLLMRDGSLLSDSTPDELL
                     ARTGALDAEAAFLHLIDTAADTAEEVV"
     gene            30411..31145
                     /locus_tag="PKU75_01490"
     CDS             30411..31145
                     /locus_tag="PKU75_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF79577.1"
                     /translation="MNPRLTLLTASRVLQQIRADRRTVALLLIVPCVLMGLLAWVFQD
                     TPVFDQVGPALLGVFPFVVMFIVTSIATLRERTSGTLERLLAGPLGKADLMFGYAIAF
                     GLVAIIQALVATAFAIYVCDLDVAGPVWVLVVIAVLDAVLGTALGLLASAFAHTEFQA
                     VQFMPAFILPQFLLCGLLVARDRLPDVLSTISDFLPLSYAVDAMSAVASQVEPLGDVG
                     PDLGIIAAWVVAALVLGALTLRRRTD"
     gene            complement(31176..31808)
                     /locus_tag="PKU75_01495"
     CDS             complement(31176..31808)
                     /locus_tag="PKU75_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPF79578.1"
                     /translation="MTPPRRRTASTPTGSRRGRRPAGTDARGDIVEAARAEFAERGYD
                     AASLRNIARRAGVDPALVHHYFEGKPALFAQVLTVPVNPGERVAMILAVPREQVGEAV
                     VRSFLFIWDAPGGRERFQALARSALGNAAVARQIREFLIAEIFVRVSRHHASAGESDE
                     DLELRASLAAGQMIGFGLMRYVLEFPAAEKVSAERAVAELGPTLQRYLAP"
     gene            complement(31814..32548)
                     /locus_tag="PKU75_01500"
     CDS             complement(31814..32548)
                     /locus_tag="PKU75_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPF79579.1"
                     /translation="MATAFALGSRLGYDGVEVMVMPDPVSRESGALAALSELHELPIV
                     SIHAPTLLLTQRIWGTDPWTKVDRSMELAQEVGASTVVVHPPFRWQKGYADSFIEGVR
                     ARESLSGIAIAIENMFPWRAGQREMLAYLPHWDPVGLDVDHVTLDLSHTATAGSDALD
                     MVEDLGSRLHHLHLADGSGAFRDEHLVPGRGSQPCGEVLETLADNGFDGAVVVEVSTR
                     KLDPTTRSGDLAEALAFARLHLATPH"
     gene            complement(32613..33602)
                     /locus_tag="PKU75_01505"
     CDS             complement(32613..33602)
                     /locus_tag="PKU75_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HPF79580.1"
                     /translation="MRLGVIDIGSNTVHMLVVDAHHGARPLPAVSHKIALRLSEHLED
                     GRVGVRAISTLTGFLDEARTLAEDQGVEQVLAFATSALREADNGEAVLTMLGEHAGTP
                     VHVLSGADEARMTFLAARRWCGWSAGTLLVLDIGGGSLEMATGMDEEPDAAISVPLGA
                     GRVTLDMLPGDPPSADTIRDARRAIRAQLASSVRPLLRAQEPDTVVATSKTFRSLARL
                     AGAAPSSAGPLVERVLTREALRGVVAQLSPLSAGQRADLPGVSVTRAPQVLGGALVAE
                     AAMDLLRVERLTICPWALREGLILRRLDTLSDAGDAADETDVGGSDTRWISVA"
     gene            33705..34508
                     /locus_tag="PKU75_01510"
     CDS             33705..34508
                     /locus_tag="PKU75_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79581.1"
                     /translation="MSETPLEIPRVWVEFDDPSDPDQRFRCDLTWLTSSWTCIFAGGC
                     QGIYADRPDDGCCTLGAHFTDEEDMGRVAAVVGDLDASTWQHRPGSTGTDAWTEPEGD
                     AIKTRVVDGACIFLNRPGFEAGPGCALHQQAVRSGLAPHTLKPDVCWQLPIRRTYRTV
                     ELPDDTSYLEVSIGEYDRRGWGPGGHDLDWYCSGNPSAHVGREPVFRSLRAELTELMG
                     EAGYAELVLRCEAHLLNIKVLRSTGARTLLPLLMHPATVEARSRRTRKR"
     gene            34521..35243
                     /locus_tag="PKU75_01515"
     CDS             34521..35243
                     /locus_tag="PKU75_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF79582.1"
                     /translation="MTPDPHSAPVDGGVRPSPNIWNHPELYEWENEAFDPDGLVEAAI
                     AALHPLAGAVVVDIGCGTGFHLLRYAALGAHVIGLEPHVPLTRRAAERVRATREGAAA
                     AIAGAAADLPFASSSVDVALARWAYFFGAGCEPGLAQVARVVRPGGIAAFLDNDATRS
                     TFGHWFTQSHPAYDPVAVQRFWSRQGFSAIGLDTRWECSDRERFEAIVRIEFEPPAAD
                     RILAEHAGTAVDYATTLWWRRY"
     gene            complement(35262..36662)
                     /gene="radA"
                     /locus_tag="PKU75_01520"
     CDS             complement(35262..36662)
                     /gene="radA"
                     /locus_tag="PKU75_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPF79583.1"
                     /translation="MAKTTRANSFRCTECGWSSVKWVGRCGECQAWGTVAEAGGATTR
                     TAAVLSPGRRPVPIGEVDPTKAAATPTGVEEFDRVLGGGLVAGGVVLVAGEPGIGKST
                     LLLDVAARAARGGRDVLYVSGEESAAQVRSRAERIEAMAKTLYLASETDLGTVLGQIE
                     QVRPALVVIDSVQTLASAQVEGAAGNVSQVREVAAALIAVAKTTGISMILVGHVTKEG
                     SIAGPRILEHLVDVVVGFEGERHSRLRLVRAVKNRFGPTDEVGCFDLSDVGIIGLPDP
                     SGLFLSTRAGSVPGTCVTATLEGRRPLLAEVQALVTPSSIPTPRRATHGVDGSRIAMI
                     TAVLEKRAGAPLTGRDIYLSTVGGVRLREPAADLAMAFAMASAILEVPIDPHLVAIGE
                     VGLAGEIRPVQGVERRLTEAARLRFSTALVPPGVRGSAALPSGIRVIEVPDIQAAVGH
                     LQRVAEQPRAELRPVR"
     gene            complement(36710..37327)
                     /locus_tag="PKU75_01525"
     CDS             complement(36710..37327)
                     /locus_tag="PKU75_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPF79584.1"
                     /translation="MTQDPQRFSSEQAKDMATGAVDKFGDQIGEMVDRAAAAIDERTG
                     SKYSEQIHAAGEKAKDALDGLDGQNDDIGAPPGGPAPPPTPAPPAEPTTPAQPAPPSE
                     PAPPTEPTQPGEPAQPSEPVPPAEPAQPTEPIQPSEPAQPTDPTPPTEPTQPTDPAFP
                     APNPPASPPDPSFPSEPSTPAIPGDPAIPSDPSIPAQPPNPSLPG"
     gene            37496..38575
                     /gene="disA"
                     /locus_tag="PKU75_01530"
     CDS             37496..38575
                     /gene="disA"
                     /locus_tag="PKU75_01530"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HPF79585.1"
                     /translation="MAQQSEEELYRSTLAVLAPGTELRDGLERILRGNTGALIVLGHD
                     RTVEQLCTGGFPLDIEFSATRLRELAKMDGAIVVDSDLSRIVRAATQLVPDPSIETME
                     SGTRHRTAERVAKQTGMPVVSVSQSMRIVQLYVGSRRHVLEGSSELLSHANQALATLE
                     RYRSRLDEVTATLSALEIEDLVTVRDVAAVLQRLEMVRRISGEVSGYVLELGVDGRLL
                     TLQLEELTTGLTDDLEWLIRDYLADGVVGDDLIRELIDGLTALSRTDLLDPASAARVI
                     GLGGPEGLDSSISPRGYRLLNKVPRLPGAVFERLVEHFGTLQRLLAATQDDLMLVDGV
                     GEGRARTIREGLSRLAESSILERYI"
     gene            38596..39453
                     /locus_tag="PKU75_01535"
     CDS             38596..39453
                     /locus_tag="PKU75_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPF79586.1"
                     /translation="MHRAVLQWYAVAGRDLPWREPSCSPWGVFVSEVMAQQTPIARIL
                     PAWQAWLRRWPTPADLAAAPVGEAIRAWDRLGYPRRAVNLHAAARAMVERHDGHVPSD
                     PEELRALPGVGDYTAAAVSSFAFGNPLVVIDTNVRRVLVRIALGQAQAAPSLTKAERE
                     LAATWQPRARSDANAWNVAAMELGALVCTARAPRCSDCPVAALCAWHQAGNPAYDGPA
                     RGGQGYEGTDRQLRGRILALLRAADHPLEPALIEALDPDPERVRRLVGALAREGLLEH
                     SRSGYHLPR"
     gene            39568..41067
                     /locus_tag="PKU75_01540"
     CDS             39568..41067
                     /locus_tag="PKU75_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPF79587.1"
                     /translation="MPATPSRPAPTRPAGALRHTAGLTILGTLIPGAGLLRTRARNLG
                     RLLLLIAGLVLAALVYKVLRDGVVGSALQVGVSRQTMLVLICAVLLGALVWMGSIVLT
                     SILARPRGMTRDERLASSIVAFLCCLAVAAPTAYAVRTLGVQRSLVAEVFGSEDGSPG
                     AGGTGTPDPGADNPWADIPRVNVLLLGSDAGTDRVGVRTDSMMVASINTRTGDTVLIG
                     VPRNLENAPIPKSNPLSTLYPDGYNCGDECLLNGIWTLAGDHADLFPGETNPGLRTIR
                     EVIGEILGLKIDNTAVIDLAGFADLVNAMGGVDINVKERVCIGCKALSDGTVVGTTDW
                     IETGPQHLDGYHALWYARSRATTDDFSRMRRQRCVMGALIKQVSPSTMLLRYPALAEV
                     LKTHVSVDIPQSQLQAWVPLIELVQGKGTLRSLPLTNKLVDVGNPDYPAIRELIADAT
                     SDNPVTSSTTASSTTSPGSAEPTGDPTTSTGTETTTEPADGLSDISTTC"
     gene            complement(41068..41361)
                     /locus_tag="PKU75_01545"
     CDS             complement(41068..41361)
                     /locus_tag="PKU75_01545"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HPF79588.1"
                     /translation="MSLPHAAVVVEDRYSSIFKLTHIRPAMVADMLAELQVQFATVPI
                     VFAENRALAQEWTYRFLGAAADESRSREARQGGGSGVELVSIVQHRTVTIQRT"
     gene            complement(41532..41768)
                     /locus_tag="PKU75_01550"
     CDS             complement(41532..41768)
                     /locus_tag="PKU75_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79589.1"
                     /translation="MEELPLTSCVPRGAAIDIVVARARESRSQFVITRARGREMIFWQ
                     SPRTSKTARPNVSLPTSRAAGIVDIAIIVDTRER"
BASE COUNT         6223 a        14657 c        15048 g         5990 t
ORIGIN      
        1 gcgaaaaccc acgtgttgtc gtaggagtcg gcgaggcggc gcagcaggaa gcgtggatcg
       61 atgggggctt cggcccgcgc gaccagcgag cgggcgagga cgaccttgtc gagggcgcca
      121 ctggtgatgc gctcgaccgc ctcccccacc gcactcgccc attcggcggc ggccttgggg
      181 ccgtcggcga aggcgatgac gcccggggac ggcggctcgg gggcggggtt caggtcgtgc
      241 ggggtcgggg ggagctggcc tccggcggtg atggtggtga gccaggcgcg gccggcccgg
      301 cgtccgacca cgacctccgg gacgaccagg cgcgagcgct ggggcgagtg cgcggcatac
      361 gccatcgagc cgaaggcgat cgggccggtg ccgggggcag cgacctcgtc gcggatgacc
      421 gcgtcggtgc acagggcgcg ccaccaccgc gcggcctggg cgaagcgatc ggcgccttgt
      481 gtctcgaagt caagagcgcg gccccagccg accagaccct cgccgcggcg cacccaggag
      541 cagacgtgct cggccgggac ctgcgcgggg atgagggtga gcaactcggc ggccgtctcg
      601 tcggggacgg cgatcgtgcg ggcgaccagg cgcgcgggct cggtggcggg cagggtggac
      661 atcgcaggga agcgtacgac gcgggccgcc gccggatcca agcgcgcacg tgagacacgc
      721 gccgcatcgg gtctatgcgt cgtggccgga ctgggagggg gcctcgccag gtcgacgacg
      781 tgacgcgtaa gggatgatgg gccggtgagc cgcgccagtc tggagaagga ccccgccgag
      841 gtcgccgcga tgttcgacga cgtcgccgcg aaatacgacc tcaccaatga cgtcctcagc
      901 ctcgggcagg accggcgctg gcggaaggtg gtcgtggagg cggtcgcggc ggcacccggt
      961 gaggcggtcc tggacatcgc ggccgggacc ggcacgagct cggagccgta tgccgatagg
     1021 ggcgtccacg tggtgcccgc ggacttctcg ctggggatgc tgcaggtggg caagcggcgg
     1081 cgcgccgacc tcgccttcac cgccgcggat gctctgcacc tgcccttcgc ggatgggtcg
     1141 ttcgatgcgg tgacgatgtc gttcgggttg cggaacgtct cggacccgag tgcggcgctg
     1201 cgggagttcg cgcgcgtgac caagccgggt gggcggttcg tgctgtgcga gttctcagcg
     1261 ccgaccaatg gggcctttcg gaccgtctac accgagtatc tgatgcgcgc gctgccaccg
     1321 gtcgcccgcc gggtgtcgtc gaatccggac tcgtatgtct atctggcaga gtcgattcgg
     1381 gcgtggccgg cgcagcggga gatggcgcag gtgatcgccg acgcgggctg gggcgagttg
     1441 cgctggcgca acctgtccgg cggcatcgtc gccgtgcacc acgcggtcaa gccggcctga
     1501 cgtcgcgtag cgcggcggcc gccacctcgc gcaggtgggc gtgggcggcc gcgtgtgagt
     1561 cgcgctcgac ccgtacctcg acgatccgga tgccgtgcgg cggctcggcg atcgcagcag
     1621 tgagctcttc ggggccggcc acgagagcgt ggggtatgcc gtgggctcgg gcgagcgtgc
     1681 ccagatcggt gcccgtggcg gtgccgaaga tccgttcgaa gggtttgcgc agcggcgggc
     1741 ggccgggctc gaggaggccg aagatgccgc cgccggcgtc gttgacgacc acgatggtca
     1801 ggtcggggcg cggctcgtcg gggccgatca gcaggccgtt gctgtcgtgc aggaaggtca
     1861 ggtcaccgag gagggcgtat gccggccggc cgggtgcgca gagggcgatg ccgacggccg
     1921 tggagacgca gccgtcgatg ccggccagtc cgcgggaggc gacgatggtg ctgcccgcgg
     1981 gcgcctgggc gacgccgaag tcgatgtccc gcaccgggtt cgagggtccg gtgaagagga
     2041 tcgctcgctc ggggagggtg gccacgagcg tggcggcgac cgcaagtcca ctgggccacg
     2101 gcggcgggtc ggcggcgacc gcttcggcga tggcgacgcc ggagtctcgc caggcgtcgg
     2161 cccacgcgct ctgaccggcg ctcgtgccgg cgcgcaggcc ggcggccagg accgcataca
     2221 gctcgtggcc ggcgttgacc acgcccacgg gatcagcgtg tgcgctggtg tccgccaagg
     2281 cctcgacccg cagtcctgga cgacgcagca gcgcagtcac cgggcgtgag agagtcagtc
     2341 gaccggccac cagaactcgt tgcggcgcaa aggaatccag gaaacgctgg tctgtcagca
     2401 ggagtggtcc gtgcgggatg actcccggct ccggaccgtg ctcgccgaag ggctcggcga
     2461 tgacgggcag gctgtgtcgg gcggcccagt cgacgacggc gggccggatg cgcgggtcta
     2521 cgagatcccc gaggatgacc agcgtgcgat caccgtcaag ggtgagatcc gttgtcgggc
     2581 aagtgttttc gccatcttgt ggcggcgtgt ccgcggaggg ttcggccggc ggcatcggcc
     2641 actgcgccgg caccagcggc tcgctgaggc agatattcag gtgcacgggt ccgggatcgc
     2701 cggccgcacc ggtggcggca cgccacgcct cccccgcgac atcccatggt gccgctgtct
     2761 catccaggtc ggcctcgagg cggacggctc cggggaagat gcccggctgg atcgtggtct
     2821 ggttcgcgcc cgaaccgcga agtgccgcag ggcgatccgc tgatagcacg atcaggggca
     2881 ccttgccgtg atgggcctcg aggacggccg ggtgcaggtt ggcgaccgcg gtcccggagg
     2941 tcgtgatgac ggccgccggg acccggctcg tcttggcgag cccgagggcc aggaaggcgg
     3001 cggaccgctc gtcgacacgc acgtgcagcc gcagccggcc ggcttgctcg gcggcatagg
     3061 cggcatacgc cagcggcgcg gaccgcgagc cggggcacag cacggcctcc cgcacaccca
     3121 tgccgacgag ggcgtcgatc aggtgggtcg cgaagtcgac ggcgggcacg ccccgatcat
     3181 gccagcgcgt gtcgcttctc aggcacgcag cgtgccggtc tcggggtcgc ggtcggtgag
     3241 gcagtaggtc tgcccgccgg gtgcccgcat gacggtccag tgcggaccag agccgcggta
     3301 tgccgcaccc aagccttcgt gcgcccgccg ggtcgcctgt cggtcggcgc aggcgaaatc
     3361 gacgtggccc gtgaccgggc cgtcaccgga gtctttgcgc tgcaacagga tccggaccgg
     3421 caggtgggag gcgcgaggga ccgcgcggaa ctccggtagg gagccgcgca ccgggtcggc
     3481 acccaggagt cgaccccaga aggcgcactc cacctcatac gaggcggcgg gaatgtccag
     3541 gcagagctgg tcgagcagat cgggctcccc cgcccgggcc cgcgcggcgg caccgtccca
     3601 ccgggtcagg cagaaaacga atcccccggg cgaacgcagg atgacgcacc cgccatcggc
     3661 atacctgaag acctcgccgg ctcccgcagc gaccacgtcc tcggcggcag ccagcggatc
     3721 ggggacgtcg aggtcgagat gaatcccgcc cccgctgcgg acctcctgca ccttgaccca
     3781 cggatccccc tgcgccggaa ggagagtcgc gaattcgtca tggtcaccac gcgtcgccga
     3841 ccgagcgctg cgcgtcgccg cctgccagaa gtcccacgcg ggtcccgcgg gcgcgagcgc
     3901 gtcgacgaag atccacatcc accgcacgtc catatgcgga ttgtgcccct cgcggggccg
     3961 ggcggcgcac gcgacatcgc tcaacgatcg tcgatctgcg ccaggacggc ggctcgccag
     4021 ccggtgggat cctcgccctc ggggacgaag agcagcgacc accacaggat cagcggccgc
     4081 caccaggccg actcggcggc cgtgagtggc cgcaccgcct catacccagc ccggaacgcc
     4141 tctctggtcg gaggccccat cggaccccaa tcgtggaacc gggtgccgag caagacggtc
     4201 gaccgcgcca gttcggcgat ggggtgatcg atgcgggcct cctcgaagtc gaggacggcc
     4261 accacgtcgc cgtctcggac caggatgttg gccgcgcggt gatcgccgtg cacgaggtgg
     4321 cgtgggccgg cgtcatcggg agcggcctcg acgagccgcc gcaggagcgc cacagctgct
     4381 gcgggagcgg ccaggggtgc ctgctcgatc caggcgccga tcagagatcg caacgacacc
     4441 ggcgaggttg cgatgccgta atgcacgtcg agaacgccct cgacccaccg ggcgnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgcac gccggccgaa gacaagcggc
     4621 agcgcacggt gtccctgact gcggcaggcg ccgccgtgtt ccggcaggcc aagcgcgaga
     4681 tccgccgcgt ggagtctgca ctcctggagc aggccttcac gccggccgag gccgccagcc
     4741 tgcgcagcca gttggagcgc ctggccgagg cctgcagcgg gcggccgagt cgcgtccaca
     4801 gcgagcgtag tgatcgcaag ccgggcggcg ggggaggcgg cggggcggca tagggtgagg
     4861 acgtgagcga gacgacgatc ctgcacaacc cgcgctgctc gacctcgcgg gccgcgctcg
     4921 agaaggccga cgccctcgga tccgatgtcg cgatccgcga ctacctcaag gagcccctca
     4981 ccgagcagga gctgcgcgac ctcgtcgcga tcctgcagga cccggtcgag gatctcgtgc
     5041 gccgcgaccc gcacttcctg gagctcggcc tggacgtcga gcacgtcgac gacgccgaat
     5101 cggtcatcgc gaccctgctc gcccacccca agctgctgca gcggccgatc ctcatccgcg
     5161 acggcgtcgc catcatcggc cgccccaagg agcgcgtcgc cgcctttctc aagggcgacg
     5221 tgcacgccga gtgatcgagc tcgtcgacgc gagcgatccg ccggcgctgc tcccgcgcct
     5281 gcgccgggcg ctcgaggggg atgcccccct gcacccgtat gccggcactc ccctccccct
     5341 gcccgcgcac gatgcggccg acctgcccgc ggacctcgcc ctcgtcgtcg gcacctccgg
     5401 gtcgacgggc gagccgaagc tcgccctgca cggcgccgcc ggactcctcg cgagcgcgaa
     5461 cgcgacgtat gcggtgctcg acggccccgg ccagtgggtg ctggccattc cgccgcacca
     5521 catcgccggc ctgcaagtgc tgatccgctc gctcgtcgcc ggcacgacgc cggtggtcat
     5581 ggaccgcacg cggggcttca ccccggcagc cttcgcgcag gcgacgcagc ggctgggtga
     5641 gggcacggca tacacctcgc tggtgccgac ccaggtgact cgtctgctgg ccgacaaggt
     5701 gggaacggct gcgctgcaac gcttttccgc ggttctcgtc ggcggcgccg ccgtgcctct
     5761 ggcactgcgg gagcagacgg gcgcgacggg cgtccggctc gtcgcgacct atgggatgag
     5821 cgagagcgcg ggcggctgcg tgtatgacgg ccgcccgctc cccggcgtcg aggtcgccgt
     5881 cgacgagagc gggcgcatcc acctcggcgg cgcgacgatc gcccacggct acctcggccg
     5941 gcccgccgag gcgttcaccc gccgcggcga cgtgcgctgg ttcgccaccg acgacctcgg
     6001 cgaactcacc gccgacgggg agttgcgcgt gcacgggcgg atcgacgacg tcatcacgac
     6061 cggcggtatg aaggtgcacc cgcgcgtggt cgaagaggcg gccctggcgg catacccgca
     6121 cctgcgcgcg gcggtggcgg tcggcatacc ggaccccgaa tggggccaac tcgtcgcgct
     6181 cgccctcgtc accgacacac cggtgaccac cgacgaagcc cgcgctcgcc tgcgcgggct
     6241 cctcccggca tacgccctgc cccgcctggt gcgcaccagc gcgaccccgc tgcccgaacg
     6301 cgggccgggc aagcccgacc gccgggccat cgcggtgtgg gtgggacaat aggggaatga
     6361 tccgcttcct gcccgggctg ctcgccctgg tcctggcgat ctatacgctc gtcgactgcg
     6421 tgcagacgcc ggaggaggcc gtgcgcggcc tgcccaaggt ggtgtgggtc ttcgtcatcc
     6481 tgctcttccc gttcgcgggg ccggccgggt ggtggttcgc ggggcggccg aagacctatg
     6541 ccctgcccgg gcgcgcgaac cccaaccagc cgccgcgccc caagggcccg gacgacgacc
     6601 ccgacttcct gcgcggcctg tgacccgccc tcatccctga cgaaggacgt tcgacacctc
     6661 ctatggccac gcctgcccaa tggatcgccg gtgcccgccc ccgcaccctg cccgccgccg
     6721 tctctcccgt cctggtcggc acgggtgcgg ccgccgccat cgaccgcgcc gacctcggcc
     6781 tcgcgctgct ggcgctggtc gtctcgctgg cgctgcaggt cggggtgaac tattccaacg
     6841 actactccga cggcatccgg ggcaccgacc acgaacgggt cggcccgatc cgactcgtgg
     6901 gccaaaggct cgcgacggcg aagaacgtca agctcatggc cttcctcttc ttcgggctgg
     6961 ccgggctcgc ggggctggcg ttgtgcgcgc tgtcggacac gctcgggctg atctgggtgg
     7021 gcgtgctgtg cgtgttggcc gcgtggttct acaccggcgg caagaacccc tacggctatc
     7081 gcggcctggg cgaggtcttc gtcttcgtct tcttcgggct ggtcgcgacc ctcgggacgc
     7141 tttacacgca ggcgcaccgc ctcaccgcgc cggggctgct cggggcgtac ggcgtgggcg
     7201 cgcttgcgac ggcgatcctc gtggccaaca acctgcgtga ccggccgacg gacgccaagg
     7261 tcggcaagcg cacgctcgcc gtgaagctgg gcgacgcgcg cacccgcgcc ctgttcgtct
     7321 tcttgacggc cacggccgtc gccgccggcg ccgcgtgcgc cctgtgggcg ccctgggcgc
     7381 tgctctgcct cggcgcactc gcgctggcga tcccgcaggt catgcgcgtc caggccggcg
     7441 ccaccggccg cgacctgatc cccgtcctgt ccggcaccgg cgtgacccaa ttgctgtatg
     7501 ccgtgctcct caccgtgggt ctctggctgg gccgctgacc agtgggcatg ctggcataca
     7561 tggtgcgcac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgggcacgg
     7681 tcgacgcctc ggtcctgacc tgtgccgagc gactgggcgt gatcgaggtg gcgaccttcg
     7741 atcgtcgcca tttcaccgtc gtccggcccg gccatgcgcc ggctttctcc ctgcttcctt
     7801 gagaatccgg caccgtcagt agcaggagta gccgccgtcg acgaccaggt tggcgccggt
     7861 ggtgaagccg gcgagatcgc tcgcgagata ggccacggca ccggcgacct cctcgggcgt
     7921 gccgagccgc ttggtgggac tgcgctccag ccacgcctca taccagccct cgggtgaggt
     7981 gccgggggtc atgtcggtgg cgatatagcc gggtgagacg gcgttgaccc ggatcccgaa
     8041 ctccgcccac tcgcacgcga gcgagcgggt gagctggatg acgcccgcct tggcggcgtt
     8101 gtaggcgacc tgctcctgcg ggtagttgac gatcagcccc gacatcgagg cagtgttgac
     8161 gatcgagccg ccacgcccct gggcgatcag ggcccgcccg gcggcccggg ccacgaggaa
     8221 gacagcgtcg aggtcgatcg ccagcacctc gcgccactgc tcgaacgtcg tgtcctcggc
     8281 cgccgagcac cgcgagatgc cggcgttgtt gtgcacgatg tccaggccac cgaagtccgc
     8341 gaccacccgg gccaccagtt caccgacggc gatctcggag cgcaggtccg cggcataccc
     8401 cacccctcgc cggccttcgg cgaccaccgc agccacggca gcggaggtgt cgagcacgtc
     8461 gacgatcgcg atgtccgccc cgcaccgggc cagcagcgcg gcggacgccg ccccgatgcc
     8521 gcgcgccccg ccggtgacca gcgcgacctt gccggtcagc gcgaacgggt ccatccgggc
     8581 gtcagcggta gtcgttggcg ccgccgtcga gggagtcctc gacggcctcg tcggagtcag
     8641 cgcgcttgga cgcgacccgc gactcgacgc gatggctcag cttgtcgacg gtgtcctgac
     8701 gcagcggccg cagggcgaaa taggagatca gcatcgacag caacgccgcg cccacgacca
     8761 gccacggctg ctcgttgcgc tcgcgcaggc cgagcaacca gagcagcccg aggcacccga
     8821 aaaagatgag gaaccgcatc agcgaatagc ggaccatgac tggtcagact cccgaatagc
     8881 tgtgctggcc gacgaaatac atgttgacga tggagtagtt gatgatgatg caggcgaagc
     8941 cggcgagcgc gatataggtc gccttctgcc gggtgacacc ggtcgtggcg cgagcgtgga
     9001 ggtatgccgc gtagatcacc cagatgatga aggtccagac ctccttggga tcccagttcc
     9061 agtaggaggc ccacgcctgc cgggcccaga tggcgccggc gatgagggtg aaggtccaca
     9121 acgggaaggc caggacgtgc agcccgtagc cgatccgctc gaggtccgac gcgggcggga
     9181 cgatccggcg cagccgtccc acgaaccctt ggctcgcgtc gggattgcgc tcggcccggt
     9241 cgatgagcaa gtaggccagc gacatcatgg ccgcgagtgt gaagacaccc gtggcaaggg
     9301 tcgcgaccgt cacgtggatg acgagccaac cggacttcag cgacggcatg agttcggcgg
     9361 cctcggtgta ccagaaggtg gtcgccgagc cgagcatcag cagcaccggg gtcgtgacga
     9421 agatgccgag ccagtcgacg ttgcgcttca gcgtccagag gcaataaccc agcaggacaa
     9481 cgaaactcgc cgccgcaacg aactcgtgaa ggttgccgag cgggggccgg tgcaccgaca
     9541 tcccccgcag gacgattgag caccccacga ggaaggtcgc gagccacgtg aacatccgcg
     9601 cgggccccgc ggccttgcgg gcacggctca gatcccggct cgccggtatg tcggtgccgc
     9661 cgggttccgc ggtcagcgcg cctcctccac cgcccaccgt gacgggctgg gccaccggct
     9721 ccggcactac cgcatcggcg gcgcgggccg cggccgagcg ggacaggtag aggacgaagc
     9781 ccagcatcgc cagggtgagc accacgatgg ccgaatagag gctgatcagc gagtactgct
     9841 cgagcgtttc cctcgtcata gtgttcaaaa tcctctcatg gcgcgattcg ggcggccagg
     9901 cgatccaggt gttcctgcag accggggtcg tcgcccttgg acagcgcccc gatctcgacg
     9961 cgcgagccgc ccgccgccag aggcgtgacc cgggcgaaga cccggcgccg cctgaccagc
    10021 agcgaggcga gcaagccggc gagcgccagc agcgaggacc acagggtcag gtccttggcc
    10081 ggatcgtgcc ggatcgacag gcccgcccag cgcacgacgg actcgaaggt gatcgacccg
    10141 cgtccgtccg gcagctgcac cgtctctccc ggcgtgatcc agatccgcag cggcttgccg
    10201 ttggcgtcgt cgaccacggt catctctttg gtgttcaggg tgtagaccga gccggaggcc
    10261 ccgccggggt tgagttcccc ctcatacagc cccagcgcca tcgccggctc gcgagcgtcg
    10321 gggaagaccg agatcggccc ctggtccttc gtgatgacgg cggtgggcaa caggttgccg
    10381 aagaacccga gctcctgcgg ctgcgccccc ggcaccttga tcgcgcccga ggaggtgtag
    10441 ttgttgtcgc gcggcaagaa gaccgtcgcg cccttgtaga cgacctcgcc gcgagagtcc
    10501 ttgacagtga cattcgcggc atacccgttg cccagcaaga agatcgtcgc cccgtccatc
    10561 tcgatggggt ggttgacctt gagcacctgc gccttctccg ccgcgcccgg ctcggcgacc
    10621 gtgcgcaggt aggcggtgaa gtcgcgcggc tgcccgaact gggcatcgcc gcgcacccgg
    10681 tcctcgaaga cggcatcgaa ccggtcgacg gtcaaggaga agggcggcag ctcggccgtg
    10741 tcgacgcggg ggccgggact gatggtgtcg tagttgatcg cggtgttgga gaagctgctc
    10801 ccgaccggga cgatgacatc gcccttccac ccgaacacgt gcccgatggc gacaccgacg
    10861 atgatcccga tgagcgcgag gtggaagagc aggttgccgc tctccttggc gaacccggtc
    10921 tcggcggcga gggtatggcc gtcgcgcacg tccacgcgat agcgccgccc gagcgctgcg
    10981 cgcaggtccg cgacggcggc ggccgggtcg gcgtcgacct cccgcgagct gtatgccgcg
    11041 agccggctca agtgcgccgg cacccggggc gggcgaccgc gcacgtggcg caggtggatg
    11101 cgggtgcgcg gcagcacgca gccgaggagg ctgacgagca gcagcaggta gatcgccgag
    11161 aaccacggcg tggcatagac ctcgaaggcg ccgagtttgt cgaggacggg tcccatggtc
    11221 gggtgggccg cgatgtagtc ggcggtgcgg gtggcgttga tggaacgctg cggccagatc
    11281 gaccccggca gggccgcgac ggcgagcagg agcagcagct gcagcgccgt gcgcatgctg
    11341 gtcaggttgc gccaagccca gcggacccag ccgaggggac cgagcgtggg ctgggtgacc
    11401 gggtcgggtt tggtcgtgat cgcctccggg gtctgggtca caggatcacc tcgaacccgt
    11461 tgatcgcgtg gatctgcagc cacgtcatga tcgaggtcca cgcgccggtg agcagcaaca
    11521 ccccgacgag gagcagcagc agaccgccgc cgatgtggat ggcgcggctg tgccggcgca
    11581 agaacgcgtt gaaccggccc gctcgctccc acgcagtcgc gatggcgagg aagggcagcc
    11641 cgaggcccag gcagtatgcc gcgacgagac tcagcgcccg gcccatgctc gggtcggtgg
    11701 ccgtcgccat cgcgagaacg gcaccgagcg tcgggcccga gcagggcgtc caccccaggc
    11761 cgaagaccgc cccgagcgcc ggggcgccga gcagccccat ccgcggccgc cagctcgggg
    11821 tgaaggtgcg ctgcgcgccg gccccgagaa agaccagcgc catgaggatc acgagcccac
    11881 cgccgatgcg gcgcagggcg tcctggtgct ccacgaacgc ccggccgatg cccgtgacga
    11941 agatcgtgat cagcacgaag acgacgctga agccgaggat gaacagcagc gtgcccgcga
    12001 ccacccggct gcgcgtgggc tccccgcgct cccccggcgc gacctcggga cccgcgcccg
    12061 cgacataccc caagaacccg gggacgagcg gcagcacgca cggcgaggcg aacgagacca
    12121 gcccggccag cgccgcgacg agcaacgcga ggggcaaggc accgctcgcg accgtctcgg
    12181 acgcactgct cgcaatcagc tggccggcga tcacttcgcg ggggcctccg cggcgacgtc
    12241 ctcgatcatg ccggtgaggg tggaggtgct cacggggccg aggatgcggg cggcaatcct
    12301 gccgtcgtgg tcgagcacca gcgtcgacgg catggccgcc gccttgccct gcagggccag
    12361 gacggcctgg ccgcccgggt cggagaggga ggggtagggc agcttccagt cctcgacctg
    12421 cgccgacccg gtcgcggctt cctcgcggaa gttgaccccg acgaactcgg ccaccgcggt
    12481 gacggccggg tcggaggaga ccgtgacgag gtcgggggcc tccttgtcgc acgggccgca
    12541 ccacgaggcc cagacattga cgaccaggac cttggcgcgg tgctcggccg aggtccacgc
    12601 ctgaccgtcg agggtcgtgc cggccagggt caccggtgtg ccgcgctggt cggcggcgat
    12661 catctcgacg gtgccgtcgc ccgcgatgaa acccttgttg ctgccctcct tggcctggtc
    12721 ggcgatggtg ttgccggagc agccggcgag agccagccca gctgcggcga gcagggcggc
    12781 gcggcgcggc agcggcctca cgcccccacg ctcgcggact cggcgtcgat caggtgtttg
    12841 gccggctcgg cataggacaa ggacgcgatc tcggtgccgt ggaaaccgat cgaggtcacc
    12901 gaggcgaggg agcacaggcg gctacgcggg tcgtgccaga gtcggcggcc ctcgaccgcc
    12961 gcccgcatca tccacacggg cagctggtgg gagacgatga ccgcttcgtg gccgatcgcg
    13021 gcctcgcggg ccgcctcgac ggcggccgtc atccgctcga cgatctcgac atagggctcg
    13081 ccccacgacg gcttggtgac gtcatacatg cggatcaggt tgcgggggcg caggaagttg
    13141 ccgaggctga cctgctcgcc ctcgaaggag ttggcggcct cgaccacccg gtcatcgagg
    13201 gtcacgggca gcccgagtcg ctccgccagc ggggcgatcg tctcctgggc ccgggtcagg
    13261 ggcgaggaca ccaggtaggt gatatcgcgc ccggcgagat ggtcggccac cacctgagcc
    13321 atcgcgtgcc cacgctcgga gaggtggaag ccgtcgagcc ggccatagag caccttgccg
    13381 gggttgtgca cctccccgtg gcgcacgagg tggaccaggg tgcgttcggt caccggttct
    13441 gcctcctcat agcgcgattg tcgcaaacga ttctgaggcg gcgcgcgcgg cggccgggag
    13501 ggccgcgagg acgcggtcga gggcggcgtc gtcgtgggcg gcggagacga accacgcctc
    13561 gtaggcgctc gggggcaggt ggacgccctg gtcgagcatg ctgtggaaga aggcggcgta
    13621 tgccggggcg ttggtggtct tcgcgtcgtc gtagttggtc accgtgcggt cggtgaggaa
    13681 gatggagaac atcgagcccg cccactggat cgtatgcggc acgcccgcgc cggtcagtgc
    13741 gctcaccgcg gcgtcggcga tcgtcttggc tgcgtggtcg aggtgggcat agacggcgtc
    13801 ggcgcagccg cgcagggtgg ccaggccggc cgcggtcgcg accgggttcc cggagagggt
    13861 gccggcttga tagacggggc cgtcgggggc gagggcagcc atcaggccgg cggggccgcc
    13921 gaaggcggcg gcggggaatc ccccacccat caccttgccg aacgtgaaca ggtcgggcgc
    13981 gcccgcggca tacgggccct cgaggccgta ccaccccgcc cgcgaacagc ggaagccggt
    14041 catgacctcg tcggagatca gcagggcgcc gtgctcgcgg gtgatgcggc gcagcgcctc
    14101 ggtgaagccg ggcagcggcg ggacgacgcc catgttgccg ggggcggcct cggtgatgac
    14161 ggcggcgatg tgctcgccgt cggtgtcgaa ggcagcctcg agcgctgcga catcgttgta
    14221 gggcaacacg atcgtctcgg cagcggcact ggccggcact ccggcggagt cgggcaggcc
    14281 gaaggtggcg acgcccgatc cggcggcggc caggagcgcg tcgacgtggc cgtggtagca
    14341 gccggcgaac ttcacgacgc gggagcggcc ggtgatgccg cgcgccagcc gcagggcgct
    14401 catcgtcgcc tcggtgccgg aggagaccag gcgcacctgc tcgaccggct cgacgcgggc
    14461 gacgatctcc tcggcgaggg cgacctcgtt ctcgctcggc gtgccatacg aaaagccgcg
    14521 gccggccgct tcggtgacgg cggcgaggac gtcggggtgg gtgtggccga ggatcatcgg
    14581 cccccacgag ccgatcaggt cgacatactc ccggccgtcg gcgtcggtca gatagggccc
    14641 gcgggcactc gtgacgaagc gggggttgcc gcccacggac cggaaggctc gcaccggcga
    14701 gttgacccct ccggggacca cggaccgggc gcgggcgaac agggcctcgg aggcgggggc
    14761 ggaatcgggt gcggacggca tacgggccat cctcgcaccc gcggccgaca ggccctcaac
    14821 ggatggcgtc gcccgcctgg ccgggctcgg cgagggcgtc gcggatcttc tgcatggagt
    14881 gcccgaggtc gcggaactcc tgcggggtga ggacgtcgag gaggtgctcc cgcaccgagt
    14941 tgacgtggca tctggccatg tcgtcgatgt gggcgcggcc ctgctcggtc aggacgagtt
    15001 cgacgccgcg ccggtcgtcg acacacgcct cgcggcgcac ccagccgcgc ttctcgaggc
    15061 gggcggccgt gtgggtgacc cggctacggg actgcacgac gaggtccgcg agggcggaca
    15121 tccgcatccg gcccgccggg gcctccgaga gcatcgagac cacttcgtat tccgagagct
    15181 gggtcccgtg gtgggccaga tcccggtcga gggcgacctc gaggagccgg ctcgcgcgca
    15241 ggtaggcgcg ccacgcctgc tgctcgtcgg gggtcagcca gcgtgtctcg ggagtcgagt
    15301 cggtcgccat tcctgctcat catgccagcg ccggacccgg ttgtggaaag tgaaagtgct
    15361 caacctcccg tggccgggaa tttgacacgg aggcatatag ttgaaatgtt aagcaccacc
    15421 acaaccaagg agaaatcacc atgcccacca tcaccgacct cacccccggc acctggaccg
    15481 tcgaccccgc ccacacggag gtcggcttcg tcgcccgcca cctgatggtc accaaggtcc
    15541 gcggcacttt cgccgacgtc gaaggcaccg tcgaggtcgc ccccgatgtg acgaagtcca
    15601 cagtcgacgt caccgtccag atggcctccg tcgacacccg cagcgccgac cgcgacaccc
    15661 acctcaagag cgccgacttc ttcgacgtcg agaactaccc gaccatgcgc ttcgtctcca
    15721 cctccttcga cggtgagcgg ctgaccggcg acctgacgat caaggacgtc accaagcccg
    15781 tcaccttcga ggtggagttc aacggcgagc aggccgaccc gtggggcaac accaaggccg
    15841 gcttcgaggc cgaggccgag gtcaagcgcg ccgactgggg cctgacctgg aacgccgcca
    15901 tcgaaggcgg cggcgtcctc gtctccgaca agatcaagat cgtcctcgac gtccagctgc
    15961 tgaagcaggc ctgacctggg cttttcgtaa ccttgggctc ccccgctaca ggagcgcggc
    16021 ggcctcgagc gcgaagtagg tcaagatgac ggtcgcgccg gcgcggcgga tgccggtcag
    16081 cgactccatc atcacccggt cgcggtcgat ccagccgcgc tcggcggcgg cgacgatcga
    16141 ggagtactcc cccgaaatct ggtatgccgc aacgggaacc gggctgtgcg ccgccaccgc
    16201 ggcgacgatg tccaggtgtg gcccggccgg cttgaccatg acgatgtcgg cgccttcggc
    16261 cacatcctgc gcgagttcgc gcagcgcctc ggtcgcgttc gccgggtcct gctggtatgc
    16321 cgcgcggtca cccacgagcg aactcgccac cgcctcccgg aacggcccgt aaaggcctga
    16381 ggtgtacttc gcggtgtacg cgagcagcgt cacgtcgcca aaaccgttgg cgtccaacgc
    16441 cgtccggacg tgaccgacct cgccgtccat catccccgac aacccgagca catgcgcgcc
    16501 cgcgcgcgcc tgggccagcc ccatcgcggc ataccgctgc agcgtcgcgt cgttgtcgac
    16561 ccgccccgcg gcgtcgagga ccccgcagtg cccgtgcgag gtgaactcgt ccaggcacag
    16621 atcggtcatc acgaccaacc ggtcgccgac cgcgtcgacg acggcccgcg tcgcgacgtt
    16681 caggatgccg tcgggatcgt cggcgccgct gccctgctcg tccttctcca ccggcacgcc
    16741 gaagagcatg atgccgccga gccccgcatc ggcgcaccga tgcgcctctg ccacaaggga
    16801 ttcgatcgta tgctgcacaa ccccgggcat ggcctcgatc ggctggggtg agccgatgcc
    16861 ctcgcgcacg aaaaccggca acaccagttg cgccgggtgc acccgggtct cggcgacgag
    16921 acgccgcatc ggcgcggaca cccgcagccg ccgcggccgg acggccggga agccgcctcc
    16981 cggcaccagc gtcatcgagc ccgccgccgt ccggctgcgc gacgccgctc gctaggccgg
    17041 cgaacggcct cgccgtgggc atgcgcctcg gcggcccggg cctggccgaa ttcggcaagg
    17101 gcgtcgatga gcgacggcac ggatgcctcg ggagccagga cgtcgacccg aagtccatgc
    17161 tcctcagcgg ttttcgcggt cgccggcccg atacacgcca ccaccgtgga cgcgtgcggc
    17221 ttgccggcga tgccgacgag gttgcgcacc gtcgatgacg aggtgaacac cacggcgtcg
    17281 aagtcgccgg ccttgatggc ttcgcggatc tccggcgcgg gtggtgccgc ccggaccgtg
    17341 cgataggccg tcgcgtcctc cacctcccac ccgaggtccc ggagcccggc gacgagggtg
    17401 tcggtggcga tatcggcccg gggcaggaag acccggtcga tcgggtccag gtcggggtcg
    17461 tagggcggcc agtcctcgag cataccccgg gcggactgct cgccgctggg cacgagatcg
    17521 ggctgaattc cccacgcgcg caacgcatcc gcggtcacgc cgccgacggc ggcgaccttc
    17581 agaccggcga aggcccgcgc gtcgagaccg acctcctcga agcgttcgcg gaccgccttg
    17641 accgcattga ccgaggtgaa gccgacccac tcgtagcgcc cggtcaccag gcccttgatc
    17701 gcgcgctcca tctggtgcgg cgtgcgcggc ggttcgacgg agatggtggg cacgacctcg
    17761 gcggtggcgc cgtgccgggc gagcaggctg atcgccggcc cggcctggtc cttcgtgcgc
    17821 gggatcagca cccgccaccc gaacagcggc ttgttctcga accacgacaa ccggtcacgc
    17881 aactcgacgg tgggaccaag cacagccaac gtcggaaact ccagtccccc aacgatcttc
    17941 gctgcctgcg ccagtcgcac cgtatgggtg cgctggcgtg tcgtcgtgcc atgctcggtc
    18001 accgcaacga ccgtctcggg gtcccggccg gcctccagca ggctcgcgaa ggcgcggccg
    18061 agttcggccg cgtcaccgaa gatgaccacc gtcgtcgacg cccccgcgcc ggctgtgaag
    18121 tcgcagccgg gatcgccggc cgcgatcacg tgaatgcccg tggtggtcgg cgcggtcaag
    18181 gggatcccgg cgtaggcggg gacagcgctg gcagccgaga ctccggggat gatgtcgacg
    18241 gcgatccccg ccttgcggca ggccgttgcc tcctcggcca gaccgttgaa gatcgacggg
    18301 tcaccatcca tcaggcgcac caccaggccg ccgggatggg cctgcgccga ctggaccacg
    18361 agcttggcgc gggaggcgtg cgtcagcggc accccatgcg agccgtgcga ggcctcgatg
    18421 atcgtggtgt ccggccggac atggcacctg atgatgtcgg cgcggtcgag ctggtcggtg
    18481 atgatcacgt ccgcccgctc gagggcgtcg gccgcagcga gcgtcagcag gccggggtcg
    18541 ccggggccgg cgccgacgaa ggcgaccgcg gccgccggcg cgttcggcgt ccggctcggg
    18601 gcggaatccg tggggctcac tggagggtct ccggggtgtc ggtctgggcg gtggacgggc
    18661 tctccgaggg ccgatgtgaa ggcgccgggc gcacctctcg gacgacatcg gcgccgtcgt
    18721 cgatgagcaa gcgcgccaat cggattcctg cgccttcggg gtcgccgagg tccccggtga
    18781 gggagcgtcg cagctctagg ctcccgtcac gcgcgccgac gaacgcgcgg atggacagtt
    18841 cggcggcgtc gaagccctcg acgacctcgg cgagaacccc gaccggcgcg atgcagccgg
    18901 cctccagttc gcgcagcacg gcccgttcgg cgcgcacaca cgcgcgggtg tcggcgtcct
    18961 ccagcgcgag gagaagcgac cggaggtcgg catcgtcggc acgacactcg accgccagcg
    19021 ccccctggcc ggcggcgggc aggatctgga tcgggtcgag cacctcggtc gcggcgtcgc
    19081 tcaggcccag gcgcagcaag cccgcccgcg ccaggacgac agcatcgacg gcaccggact
    19141 caaccagccg caatcgtgtc tcgacattgc cgcggaccgg cacgacgtcg agccccaggc
    19201 cgagcgcgtg cagctgcgcc tcgcgtctcg gtgagccggt gcccacgcgt gccccccggg
    19261 gcagctcacc cagcgtcaac ccgtcccgag cgaccaggac gtcacgcacg tcctcgcgca
    19321 ccggcactgc cgcgatcgtc aggcctgatt cggccgctgt cggcagatcc ttgagcgagt
    19381 gcacggcgaa gtcgatctgc ttctcgcgca acgcggtccg cagagcggac gcgaaaaccc
    19441 cggtgccgcc gatctgatcg agcgccgccg tcgaccggtc gccctcggtg acgatctcga
    19501 cggtctcgac ctcatgcccc agggcccgca gggcgtcggc aaccgtgccc gactgggtca
    19561 gggccagccg gctgcgtcgc gtgcccaacc gtagtgccgt catcgggcac ctccggccgg
    19621 gtcgcggtcc gcggcgggct cgaagggcgg gggcacgctg accgacgcga ccgtctcggg
    19681 ttcgagcgcg aacaactcgg cgagggcgtc ggcatacgct tcgatgcggc cgctggctgc
    19741 gagttctttg acgcgcaagg tcggcccgtg cagcaatttg tcgacgaggc gccccatcgc
    19801 gatctcgacc tcgcgccggg tggcgtcgtc gacgtcgggg gtgcggccgg cgagccgggc
    19861 cagttccgtg tcgaggacct cggtcgcccg ggcgcgcagg gcggcgatcg tcggcgcggc
    19921 ggcctgcgcc gagcgggccg tgaggaaggc cgccacctcg ccggtgacga tgtcggtcgc
    19981 cgcggcgacc tcggggtcga ccccgtcggt atgcagtgtc tccgcgagat gttcgaggcc
    20041 gacgcggatc gcgccgggca ggtcggcgac cgcatgggag acgtcatggg gcaacgccag
    20101 atcgatatat gcctgcggcc ggccctcgcg gtgggtgagc gcctcggcgg ccatcgcctc
    20161 ggtgatcacg acacccggtg acccgacgca ggcgatgacg atatcggcgg ccgcgagagc
    20221 ctgcgggagc tccgagagcc ggaccgcgtc gccgcccgcc cgctcggcca acgcctggcc
    20281 ccgggccagc gtgcggttcg cgatcgtcag ccgtcccgcg cccgcgcgcc gggctgtgat
    20341 cgcggccagg gctgccatcg cgccggcgcc gacgacgagc acccgagtgt cctcgatcgg
    20401 cccgacatgc gcgccggcaa ccgcgagtcc cgccccgacc agtgatcccg ccacgttggc
    20461 cagcccggtc tcggtgtgca cgcgtttgcc cacccgcaag gcctgctgga agaggccgtt
    20521 gagcgccggc ccgacgaccc gctcctcttg cgcctcatgc agcgacgccc gcagctgggc
    20581 gcgaatctgg ctctccccca ccgccatcga gtccaggccg cccgcgaccc ggaaggcgtg
    20641 cgcgatggcc cggtcctcgt agtgcaccgc caggcgctcg gtcaggtcgt ggcgttcgac
    20701 accggtgacc gaccccagtg cctccgtgac ggccgacacc gctccgtgga aggtcaccgc
    20761 ctcggtgtag acctcggtgc gattgcacgt cgagacgaca acgacctcgg cgacgtgctc
    20821 gctgccggcg agttcgtggc cgagggcgcg gcgagcggca ctgtcgaggc tgatgcgttc
    20881 gagcagatcg agggggcagt tgtggtggga caggcccaag acgagcaggc tcacggggcc
    20941 accgccaggg ccggcgcggc cgtgcggcgc tgcttgtgga aggcgaggat ctgcagctcg
    21001 ctggcgatat cgacgcggcg cacatccacc ggcgccggca cgacgatggc cgccggggcg
    21061 cagttgagga tcgaggtgat gcccgccgcg accagctggt ccgcgaccac ctgcgcggcg
    21121 gcggcgggcg tggcgatgac tcccaccgtg tcggcagccc ggggcaggtc ggcgatggcc
    21181 gacatgggcg agatcgtcag gcccgacacg ctctgcccga tcaccgccgg atcggcgtcg
    21241 aagaggccgg cgaccgagaa cccgcgagag gagagcccgg catgcccggc cagcgcccgg
    21301 ccgaggttgc cgagaccgac gatgacgacc ggccagtccc gggtgagccc cagctcacgg
    21361 gagatctcga cggtcaggtg cgccacgtca tacccgaccc cgcgcacgcc atggctgccg
    21421 aggtgcgaga ggtccttgcg caccttcgcc ggatgcacgc cgacggcttg ggccagttcg
    21481 agggaggagg tggaccggac gccggcttcg gccatgatcc ccagcacccg caggtatgcg
    21541 ggcaatcggg cgacggtggc gtcggggatt ccacgacgcg gcaggtcggt cgcaggcacg
    21601 ccgtcgcctc cagtcacctc gcgggtacat gtgtcggccc gtacggctag gcgccgggag
    21661 ccactacgga ggttacgcgc ttgtgaagga ctgcacaaag tccgatcggt ccgtgagtcg
    21721 ccgctcacag ccgccggacg agggccgcac gcagccggtc ggcgtcgatg cgccagtagt
    21781 cgtggacggc gccgtcgagg agtgtgaccg ggatcatttc gccgtatgcc gcggcgaggt
    21841 cagggtgatc gcggatcgag acctcctcga ccccgacccc gaactcttcg gccagggcga
    21901 gcacgaccgg tcgcacggtg tcgcacaagt ggcagcccgg cttgccgatg agcgtcaagg
    21961 gggctgtcgg tgtccacggg ccgggcgcgg cagcggccat cagaagaagg cggagcggcg
    22021 ctgcgtgagc aggccgtaga ggctctgctg gatgccctcg cggatgcggt cggtcaggtc
    22081 gaagacgatc atcgggtcgt cggccgcccc ggcacccagg tgggcggtct cgaccggcgc
    22141 cccgaactcg atgatccact tgctcggcag cggaatcgca cccagcaacc cgaagtgcgg
    22201 gaagagcggc gtgaccggga aatagggcag gccggtcagc cgggcgaggg gtttgagatt
    22261 ggccagcatc ggatagatct cctcggcgcc gacgatcgag cacggcacga tcggcacgcc
    22321 ggtgcgaatc gccgccgaca cgaacccgcc gcggccgaag cgctgcagct tgtagcgctc
    22381 ggagaacggc ttgccgactc ccttgaagcc ttcggggaag actccgacga gttccccgtc
    22441 cgccaggagc cgctcggcgt cgacattggt cgccagcgtc gagcctccca tgcgggcgat
    22501 cgtcgccagg cccggggtgg agaagaccat gtcggccccg agttcgcgca gcgcccggtg
    22561 ctgcggatgc tggtcgcgga tggcaactcc gaccatgagc gagtcgacgg cgatggtgcc
    22621 ggagtgattt gccactagca gcacgccacc gtcggcgggg atgttctcga ctccccggac
    22681 ctcggtgcgg aaccacttgt agaacagcgg ccgcagcagc ggcatgagga cgtgatcggt
    22741 gaagtcggag tcgaaaccgt aggcatcgac ggtgaaatcg ccggtcaggc ggcgccggag
    22801 gaaggccagc ccttcggcga ccttgcgttc ggcgtcttcg cgggggatgc ccagcgcgga
    22861 cgcgagggtg gtggcaaccc cctgggccgc gtcgacgatc gcgccgaggt cgggtgccgc
    22921 cgcctggggc gcgctctcgt cgggaccgac cactcgcagg tgcggcggtt cgggcgcgtc
    22981 cgccagcagg ggggtggtgc ggtggcgggc acgctccccg ctggcgcgcg cggcgccggc
    23041 agccacggag gcccgccgtc gccgccgggc aggagcggtc gaggtcgtcg gctgcaccgc
    23101 ggtcttggcg cgcgtggcgg cttttggcgt ggttttggac gcagcggact tggaggcggc
    23161 catctcagcg gttccgtccg atcggcaccg ctccgacgag cgagtgcagt gctgaaccgg
    23221 cgtcgtcgac ggcgccggca attgcggtga gtgcggtgtc gaccggccgc ggcgcggcgg
    23281 cgacgaagtc ctcgaaggca gccttggtgc tgtatgccgg ttcgaaaccg agcaccgtct
    23341 tcatccgggt cgtgtcgagg ccgcggccgt agacgagcag gtcgagttgc tcgggtgaga
    23401 agtcgatcag gccggtgcgt ttgaagatcg tcgagagccc ggtgctcagc ggggtcggaa
    23461 tcgtcacgaa aggtcgcccg gcaagggcga tcgcctggct gacggtgatg atcccgtcac
    23521 cggcaacgtt gaccgtgtcc gccggctcgc cgagtgtcgc cgcgatcatg gcggccgcca
    23581 catcgtcctc gtggaggaac tgcagccggg catcccaccc gagcgggatc ggcacgaccg
    23641 gcaggcggaa gtagtcggtc atggccgtgc gcacccgcgg gccgatgaca ttggccaggc
    23701 gcagcgtggt gatgccgacg tcaggccgcc ggcgtgcgaa cccccgcacg taggcctcga
    23761 cgtcgaccga gtccttgccg aagccgagtt tgggaagccg cttggcggcc atctcctcgg
    23821 tgaacagcgc cggatcgcgg tgcgaggcac cgtagacccc ggccgtcgac ttcacgacga
    23881 gcttgcgcac gcgcggtgcc ttttggcagg ccgcgagcaa ctgcatcgtg ccgatgacgt
    23941 tgatctcttt ttgggtggcc cggccacccg cgccgagcgg agtggcgatg acgcccatgt
    24001 gcacgacggt gtcgacggca tactgctcga tgagcttccc gatcatcggg ctgcggatgt
    24061 cggcgcgcac gaactcggca cgcccgatgt cgtggggtgg cggtatgacg tccaccccca
    24121 cgatcgtgcg cgccgggtcg acctgggaca gggcgcgagc aaaagcgcct ccgaggtatc
    24181 gagagactcc ggtgatcagg acgacatcag ccatcgcgcg cctcacccgc ttgagctggg
    24241 actacttctt gttgcgacgc tggtgacgcg tcttgcgcag cagcttgcgg tgcttcttct
    24301 tggccatccg cttgcgccgc ttcttgatga ctgaacccat atcgagcctt tgatcgaaag
    24361 aacaggaaaa gtggtcgtat gccgtgtgcc cgccaccggc gaacgctcga ctatcgaccc
    24421 cccagcttag ccgccgcgca gggggcaccc aaactgcgtg atggtgatgt ggcggacacc
    24481 gcgggttcgc gggcatacgc acgaccgccc gctcactcgg agcggacggc cgggatcgcc
    24541 gcggacggcg agagggtgat gtcggcggcg gctacgcagt ctcgatgtag gaggacttca
    24601 agtaggcgtc gacggcatcc tcgggaacac ggaaggagcg tccgacacgg atggcgggca
    24661 gctcaccgcc gtggaccatc cggtagacag tcatcttgga cacgcgcatg atggccgcca
    24721 cctcagcgac cgtgaggaag cgcacctcgg agagctgccg atcggcagtc atagatacct
    24781 cgtcattctc accgcgcatc gccggggttc ccgtccggcg gcctcctacg cggaactgtc
    24841 gagagcaacc ataggggcag ttgtgactcg tgggaagcgg gcagggcagg ctaagacaaa
    24901 tcggtacgaa acgcccccac cgacacgccc ttgtccccct ggttcttggc ctcctcgcgc
    24961 cccgccaccc gggcgacccg agcccgccgc ggaggggaac cacccaggtg gcgacagcag
    25021 cgacaaggcg gggcgacgca agcggcccca gtgannnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnatgcgg gcgaggatcg gccgcgccag cgggcgaacc gagctcgccg
    25201 aggaggcgaa ccagaggatt cagtcgtacc agatgtcgag gccgtggtgg ctgaaggtgg
    25261 cctcgcgggt ggccatgatg gcgcggtcga cggggctgtg ggggttgtat cccatctccc
    25321 acggctgcac ccagatggcg ggttcgccga cggggaggtc gcccatgcgg ttgggtccgc
    25381 ggcggccgat ggtgcgttcg acgtactcct gccactcctc ggggacgggg gtgtcgacgg
    25441 ggatgggccg gtgggcggcg atggcggtca tatgggtcca cgatcgcggc acgacgtcga
    25501 ccagctcata tcccccgccg ccgagggcga cccacttgcc gtcgcacagg ttgtgggcga
    25561 ggtcgtggat gtccagcatg gcctggcgtt gcgcgtcgac ggtcatgacg aggttggcca
    25621 gcgggtcgtc ggcgtgggtg tcgcagccgt gctgggagat gaggaattgg ggccggaagg
    25681 cgcgcaggat ggggacgatg ctggcgcgga tggcgcgtag ccacgccgag tcggtggtgc
    25741 cgggcgggag ggcgacgttg accgcatacc cttcgccggc tccggttccg acgtcgcgcg
    25801 cccacccggt gccggggaag agcgtccgcc cgctctcgtg gatgcttgcg gtgagcacgc
    25861 gggggtcgtc ggcgaagatc gcttcgacgc cgtcgccgtg gtggacatcg acgtcgacat
    25921 aggcgacgcg ttcgacgccg tcgtcgagca gcgcctggat gccggcggcg atgtcgttgt
    25981 agatgcagaa gccggaggcg ttgccgcgca tcgcatggtg caggccgccg cagaagttga
    26041 cgccgtgcgc gatctcgccg gcctggacct gctcgcacat cgccacggtg ccggcgacga
    26101 tgcgagcgct ggcctcgtgc atgccggtga aggcggggtc gtcctcggtg cccaggccgt
    26161 atgccggctc ggccgagctg ggatcgtccg aggcctcctt gaccttggcg atgtagtcgg
    26221 ggtcgtggac ggtctggagc atttcgtcgc tcgccacggg cggggagatc acctcgacgg
    26281 cgtcgaagac gccgagggct tcgcacaggc gggcggtcag gtccaggcgc agcggaccca
    26341 tcgggtggcc ggggccgaag ttgtatgccg tgaagcccgg atcccagatg acgcgcgcct
    26401 gtgcgaccat gcaacgcacg ctaccgcttg tgccgcaggt cggcatgctc aggtgacacg
    26461 acggaatgga gggctcgggt gggcaaaagg cagcacgacg tcctggtcat cggcctgggc
    26521 cggttcggcg gcgccgcggc cctggagttg aaccggctcg ggcaccgggt gacggcgctc
    26581 gagaaggacc cggtgatcgc cgagtccttc ctcggcaagg tcttcaaggt cgtgcacgcc
    26641 gacgccagcc tgccgcacgt gctggccgag cagaagtgcc agaacttccc ggtcgcgatc
    26701 gtcgggatcg ggttctcggt cgagtcgtcg gtgctggccg ctgccaacct cgtcgacgcc
    26761 gacgtccctg atgtctgggc caaggcgctc tcgccggaac atgcccggat cctgcagcgg
    26821 atcggcgtcg ggcacgtcat cctgccggag gccgagaccg gtcgccgggt cgcgcacctg
    26881 gtgggcggtg ggttgctgga ctacatggag ttcgacgacg gcttcgcgat ggtgaagatg
    26941 gcgccaccgt cggaggcggt gggttttacg ttggcgcaga gcgctattcg ttccaagtac
    27001 ggcgtcacga tcgtcggcgt caagtcgccg ggtcgcgatt tcgtgcacgc cacccccgaa
    27061 acgaaggtga cctcgcacga caccttgatc gtcagcggtc cgaccgctct gatcgagcgc
    27121 ctggccgccc ggccctagga cacccgggcc ctcggacacc ccacgacgtt tccgcactcc
    27181 ttcgtcgctc cggtgcttct cggggacccc gcactggtgg ggtttcgcaa cacggcctgg
    27241 ggttcagccc gcgcggtcct cggcgagcgc gaggaccatc gcgatctctt cttcgccatc
    27301 ggcaccgttg gtcacccgca tcgggcggcg gcgtccggtt ggccggaagc cgaagctgga
    27361 ggcgaaggcg acggcgcggc cgttgtcggt gctcacccag taggcgatcc ggtcgtagcc
    27421 gtcctcggcg gcctgctgcg aagccgaccg gaccagggtg gcggcgacgc tgcggccgcg
    27481 ccagtccggg cgcacccaca ggccgaagat ctcagcggtg ttctcggcgt catcgagcac
    27541 accgacgctc agcacgccga catcggatcc gtccacgtcg gcgaccatgc gctgggcccg
    27601 acgcatccgc gcgcgccaga agtcctcgtc atactcctgc tcgtcggcga gtttggcgac
    27661 gaaagcctcc ggggattcct cgagagcagc cagccggatg gcgcggaact gcccccagtc
    27721 cgcctcggtg agcggacgca cgcggatctc tgtcatgggc acatcctgac atgtgccgcc
    27781 gacatccgcc ccctctcggg aaaacggcga tcaggtctcc gcgagctcgc gcgatcggcg
    27841 ggccgcggcc tcgatggcgc tgacgaaggc ggccctgacc ttgtgatcgt cgagggtgcg
    27901 caaggcggcg gccgtggtgc caccgggcga ggtgacctgc tcacgcagca ccgtcgggtg
    27961 ggtgccggtc tcgcggatca tcgtcgccgc gccgaacagg gtctggaccg tcagctctgt
    28021 cgccgtctcg cggggcaggc cgagcacgac gccggcctcg atcatcgcct cgacgacgta
    28081 gaagacgtag gcggggcccg acccggagat cgccgtgacg gcgttctggt agccctccgg
    28141 cacctcgatg gcgcggccgc acgatcgcag cagcgacatc gcccgctcca tctgctcgtc
    28201 ggtgacgtgg gcaccgcggg aaaacgcggc cataccctgg tcgacgagag ccggggtgtt
    28261 gggcatgacc cgcaccaccg gagtgccctc gggcagcccc gcggcgatgg cctgggtcgg
    28321 gacgcccgcg gcgagcgaga cgacgaggtt gccaggtgcg atctggtcac cgatctcggc
    28381 gagcagagcg gtcatgtcct gcggcttgac cacgaagacg agaacgtcgg cgacggccgc
    28441 accctcggcg ttggtcaggg cgcgcacgcc gtacttggcc tcgagttcgg cggcccggtc
    28501 ggcacgccgc tcggtgatga cgacctcgcc gggggccaat ccggaccgca gcaggccgga
    28561 gagcagggtc tcccccatca caccggcacc gaagatggca atggtcacgg cgaaattcct
    28621 cctaggagcg cgacaacagg gagcggacga acagcatcgc attggtcggc cgttcagcca
    28681 tccgccgcat caaatagccg taccactcct cgccgtaggg gatgtagacc cgcatgcggt
    28741 ggccgcggtc ggcgaggcga cgctgctcct cggggcgaat gccgtagagc atctggaact
    28801 cgaagctgtc cgcggcccgg ccggcgctct cggcgagcgc ggtcgcgatg tcgatgagcc
    28861 gggggtcatg gctggcgacc atgggatagc cggcgccgtc catgaggatc cgcaggcacc
    28921 gcacgaacga cttgtccacc tcatcgtcgt cctggaaggc gacggtctcg ggctcgttgt
    28981 aggcaccctt gcacaaccgc actcggctgc cggccgccgc caagtcgcga cagtcggctt
    29041 cggtgcggtg caggtaggcc tgcaccgcca ccccgaccca cgggtagtcg cgccgcaact
    29101 cccgcacggc cgcgagggtc gcgtcggtcg tcgtgtggtc ctccatgtcg acggtgacag
    29161 tggtcccggc acgctcggcc gccgcgcaga taatccgaac gttctcgagg gcgatcgccg
    29221 cgccctcgtc gggcaagagc tgcccgacag cgctgagttt gacactcacc tcggcccagc
    29281 caccgtgcgt atgttcttct gccgcaagca gatcgatgag actgacgtat gcctgcgtca
    29341 ccgcctgggc ctgggcgcga tcggtggtgt cctcccccag gtgatcgatc gtcgcgcgcc
    29401 ggtcggtagc gcgcaactcg gcgacggcag caaccgcgtc ggtgtcgtag gcgccgggga
    29461 cgtagcggcg caccacggtg cggctgacgg gggcccgctc gatcagatca cgcacgcgcg
    29521 tgtcggcggc gagagtgagg agtgtcttgc ggagaagttc ggacggaccc atggtcgtca
    29581 ggctaacgac ccacgacata cagccaggca gtggtcgaag cggactttca gatcggacac
    29641 ctggcgcgtc gccctctctg gtggtatttt catcacatga tgaataccgc ggcgatcgtc
    29701 atcgacgatc ttctggtccg ccgtggcgga gtcgtcgtca tcccgggcct acgggtctgc
    29761 gtccccgccg gccaggtggt gggcctgctc ggccccagcg gcagcggcaa gtccacgctg
    29821 atgcgggccg tcgtcggcgt ccagaagatc gccggcggca ccgtcaccgt cctcgggcag
    29881 gtcgccgggt cccccgccct gcgctcccgc gtcggctatg tcacgcaaag cccgagcgtg
    29941 tatgcagacc tgaccgtccg cgccaatgtc gcctacttcg ccgggctcgt cggacagggt
    30001 tcggacgccg tcgacaaggc cctggccgag gtcgacctga gcgccgtcgc cgatcggaag
    30061 gtggagcgac tctccggtgg acagcgctcc cgcgtctcgc tcgccgccgc cctggccggc
    30121 gaccccgaac tgctcgtcct cgacgagccc acggtcggat tggacccggt gctgcggcgc
    30181 gacctgtgga acctgttcca ccgcctggcc ggcgccggcc gttcgctgct cgtgtccagt
    30241 cacgtgatgg acgaggcgac ccgctgcgat cggctgttac tgatgcggga cggctcgctg
    30301 ttgtccgact cgacgccgga tgaactgctt gcccgtaccg gtgcgctcga tgccgaggcc
    30361 gccttcctcc acctcatcga caccgccgcc gacaccgccg aggaggtcgt gtgaacccgc
    30421 gcctgacatt gctcaccgcc agccgggtgc tccagcagat ccgcgccgat cgccgaacgg
    30481 tcgccctgct gctcatcgtc ccgtgcgtgc tcatgggcct gcttgcctgg gtgttccaag
    30541 acaccccggt gttcgaccag gtgggaccgg cattactcgg tgtcttcccg ttcgtggtga
    30601 tgttcatcgt caccagcatc gcgacgctgc gcgaacgcac gagcggcacc ctcgagcgct
    30661 tgctcgccgg gccgctcggc aaggccgacc tgatgttcgg ctacgcgatc gcgttcggcc
    30721 tggtcgcgat catccaggcg ctcgtggcga ccgccttcgc gatctacgtc tgtgatctcg
    30781 acgtcgccgg gccggtgtgg gtgctcgtcg tcatcgccgt cctcgacgcc gtcctcggga
    30841 ccgccctcgg attgttggcc agtgcgttcg cgcacaccga attccaggcg gtccagttca
    30901 tgccggcctt catcctcccg cagttcctgc tgtgcgggct gctcgtcgcc cgggaccggc
    30961 tgcccgacgt gctctccacg atttcggact tcctgccgct gtcgtatgcc gtcgatgcga
    31021 tgtcggccgt cgcctcccag gtcgagcccc tcggtgacgt cggtcccgat ctcggcatca
    31081 tcgcggcgtg ggtggtggct gcgctggtcc tgggcgcgct caccttgcgg cgccgaaccg
    31141 actgagcacc cccggctgcg gacggccgcg gccggtcacg gggcgaggta gcgctgcagg
    31201 gtcgggccaa gttcggcgac cgcccgctcc gcggaaacct tctccgcggc cgggaattcg
    31261 aggacgtagc gcatcaggcc gaagccgatc atctggccgg cggcgaggct ggcccgcaac
    31321 tcgaggtctt cgtccgactc gccggcgctg gcgtggtggc gggaaacccg cacgaagatc
    31381 tcggcgatga gaaactcgcg gatctgtcgg gcgaccgccg cattaccgag ggcggaacgg
    31441 gccagcgcct ggaagcgttc gcgaccgccc ggggcatccc aaatgaaaag gaaggaccgg
    31501 accacggctt cgcccacctg ctcgcgaggc accgcgagga tcatggcaac gcgttcgccc
    31561 gggttgacgg gcacggtgag gacctgggcg aacagggccg gcttaccctc gaagtagtgg
    31621 tgcacgagcg ccggatcgac accggcccgg cgggcgatat tgcgcagcga cgccgcgtca
    31681 tacccccgct cggcgaactc ggcacgcgcg gcctcgacga tgtcgccacg ggcgtcggtg
    31741 ccggccggtc ggcggccgcg gcgcgaccct gtcggggtgc ttgctgtgcg tcgtcgtggg
    31801 ggcgtcatgg ccgtcagtga ggggtggcca gatgcagccg cgcgaacgcc agggcctcgg
    31861 ccagatcacc gctgcgggtg gtcggatcga gtttgcgggt cgagacttcg acgacgacgg
    31921 caccgtcgaa tccgttgtcc gccagggttt ccagcacttc accgcagggt tgcgagccgc
    31981 gtccggggac caggtgctca tcgcggaaag cgccggaccc gtcggccaag tgcaggtggt
    32041 gcaggcggga tccgagatcc tcgaccatgt cgagggcgtc ggagccggcg gtcgccgtgt
    32101 gcgacaggtc gagggtcacg tggtcgacgt caaggccgac ggggtcccag tgcggcaagt
    32161 aggcgagcat ctcgcgctgt ccggcccgcc acgggaacat attctcgatc gcgatcgcga
    32221 tcccggacag ggactcccgc gcccgcaccc cttcgatgaa gctgtcggca tagcccttct
    32281 gccagcgaaa cggcggatgc acgaccacgg tcgacgcgcc gacctcctgg gcaagttcca
    32341 tcgaccggtc gaccttcgtc cacggatcgg taccccaaat ccgttgcgtc agaagcagag
    32401 tcggggcgtg gatcgagacg atgggcagct cgtgcagctc ggacagggcc gccagggcgc
    32461 cgctctcgcg actgaccggg tcgggcatga ccatgacctc gacgccgtca taccccaggc
    32521 gcgagccgag cgcgaacgcg gtggccacgg ggtcgggata gaccgacgct gtcgagagcg
    32581 cgacaggggc agccgggacg cgtgcggctt gctcaggcaa cgctgatcca cctggtatcg
    32641 gaaccgccga catcggtctc gtccgcggcg tcgccggcgt cggacagggt gtcgaggcgg
    32701 cgcaggatga gcccttcgcg caacgcccac ggacagatcg tcagccgctc gacccgcagc
    32761 aggtccatcg ccgcctcggc gaccagggca ccaccgagca cctgcggcgc gcgggtcacc
    32821 gagaccccgg ggaggtcggc gcgctgcccg gcggacagcg ggctcaactg ggcaaccacc
    32881 ccgcgcaggg cctcccgcgt cagcacccgc tcgaccagcg gacccgcgct gctcggcgcc
    32941 gcgccggcga gccgggcgag cgagcggaag gtcttggagg tggccacaac ggtgtcgggc
    33001 tcctgggcgc gcagcaacgg gcggaccgag gaggccagct gggcacggat ggcgcggcgg
    33061 gcatcacgga tcgtgtccgc ggacggcggg tcgccgggga gcatgtcgag ggtgacccgc
    33121 cccgcgccga gagggacgga gatggcggcg tccggctcct cgtccatccc ggtcgccatc
    33181 tccagagaac ccccaccgat gtcgagcacc aacaaggtgc ctgccgacca gccgcaccag
    33241 cggcgggcgg cgaggaaggt catccgcgcc tcgtccgcgc cgctgagcac atggaccggc
    33301 gtgccggcgt gctcgcccag catcgtgagc acggcctcac cgttgtccgc ctcgcgcaac
    33361 gcactcgtcg cgaaggcgag cacctgctcg actccctggt cctcggcgag ggtgcgggcc
    33421 tcgtcgagga agccggtcag ggtgctgatc gcgcggaccc cgacgcggcc gtcttcgagg
    33481 tgttcggaca ggcgcagcgc gatcttgtgc gagaccgcgg ggagcgggcg cgctccgtgg
    33541 tgggcgtcga cgacgagcat atggacagtg ttggagccga tatcgatgac acccaggcgc
    33601 accacgccag actaaccgtc gccacaggcc ggcggtctcc gcgcgccggg cgcgtgccgc
    33661 gccggcgcgc catcacagcg ccgttgtgct tacagttggc catcgtgagt gagacgccgc
    33721 tggagatccc ccgggtgtgg gtggagttcg acgatcccag cgaccccgac caacgcttcc
    33781 ggtgcgacct gacctggttg acctcgagct ggacgtgcat cttcgccgga ggctgtcagg
    33841 gcatctacgc cgaccgcccc gacgacggct gctgcaccct cggcgcccac ttcaccgacg
    33901 aagaggacat gggccgcgtc gccgccgtcg tcggggacct ggacgcctcg acctggcaac
    33961 accggcccgg atccaccggc accgacgcgt ggaccgaacc ggaaggcgac gccatcaaga
    34021 cccgcgtcgt ggacggggcc tgcatcttcc tcaaccggcc cggcttcgag gccggcccgg
    34081 gctgtgcgct gcaccagcag gcggtccgca gcgggctggc accgcatacg ctcaagccgg
    34141 acgtgtgctg gcagctgccg atccggcgca cctaccgcac cgtcgaactg cccgacgaca
    34201 cctcgtatct cgaagtgagt atcggagagt acgaccggcg cggttggggg ccgggagggc
    34261 acgacctgga ctggtattgc tcgggcaatc cgtccgcgca cgtggggcgg gagccggtgt
    34321 tccggtcgtt gcgtgccgag ctgaccgagc tgatgggtga ggccggatat gccgagttgg
    34381 tgctgcgctg cgaggcgcac ctgctcaata tcaaggtgtt gcgttccacg ggcgcccgca
    34441 cgctgttgcc gctgctgatg cacccggcga cggtggaggc ccgctcccgc cgcacccgca
    34501 agcgttgagg ctgcccggcg gtgactccgg acccccatag cgcacccgtc gacggcgggg
    34561 tgcgacccag cccgaacatc tggaaccacc ccgagctcta cgagtgggag aacgaggcct
    34621 tcgaccccga cggcctggtc gaggcggcaa tcgcggcgct gcacccgctg gcgggggccg
    34681 tcgtggtgga catcggatgc ggcaccggtt tccaccttct ccggtatgcc gcgctcggcg
    34741 cccacgtgat cggtctcgaa ccgcatgtgc ccttgactcg ccgcgcggcc gagcgggtac
    34801 gtgccacccg ggagggtgcc gcagccgcaa tcgccggtgc cgctgccgac ctgccgttcg
    34861 cgtcgagcag cgtcgacgtg gcgctggcgc ggtgggccta cttcttcggt gccgggtgcg
    34921 agcccgggct ggcgcaggtg gcacgcgtcg tgcggcccgg cggcatcgcc gccttcctcg
    34981 acaacgacgc gacccgctcc accttcgggc actggttcac gcagagccac ccggcatacg
    35041 atcccgttgc ggtgcaacgg ttctggagcc gtcaagggtt cagcgccatc ggcctggaca
    35101 cgcgctggga gtgctctgac cgggagcggt tcgaagctat cgtgcgcatc gagttcgagc
    35161 cgcccgcggc agatcgcatc ctcgccgagc acgcgggcac ggcggtcgac tacgcgacca
    35221 ccctgtggtg gcgccgctac tgaccggccg gctgcggccg gtcagcggac cggccgcaac
    35281 tcggcgcggg gctgctcggc gacccgttgc aggtgcccga cagcggcttg gatgtcgggg
    35341 acctcgatga cccggatgcc cgacggcagg gcggcgctgc cgcggacccc cggcggcacg
    35401 agcgctgtgc tgaaccgcag ccgggcggcc tcggtcaggc gccgctcgac tccttggacc
    35461 gggcggatct cgccggcgag gccgacctca ccgatcgcga ccaggtgcgg atcgatgggg
    35521 acctcgagga tggcgctggc catggcgaac gccatggcca gatcggccgc cggctcgcgc
    35581 aaccgcaccc ctccgacggt ggagaggtag atgtcccggc cggtcagtgg tgcaccggca
    35641 cgcttctcga ggacggccgt gatcatcgcg atgcggctgc cgtcgacgcc gtgggtggcc
    35701 cgacggggtg tcgggatcga actcggggtg acgagcgcct gcacctccgc cagcagcggg
    35761 cgccggcctt cgagggtggc ggtgacacag gtgcccggca ccgaaccggc ccgggtggac
    35821 aggaacagcc cactcgggtc gggcagcccg atgatgccga catcgctcag gtcgaagcaa
    35881 ccgacctcgt cggtcgggcc gaagcggttc ttgaccgccc gcaccagccg cagccgcgag
    35941 tgccgctccc cctcgaaacc caccacgacg tcgacgagat gctcgaggat gcgcggcccg
    36001 gcaatcgacc cctccttggt gacatgcccg acgaggatca tcgagatgcc cgtcgtcttg
    36061 gcgacggcga tgagcgccgc ggccacctcg cgcacctggg agacgttgcc ggcggctccc
    36121 tccacctgcg cgctggccag cgtctggacc gagtcgatca cgaccagcgc cggacgcacc
    36181 tgctcgatct gccccaagac agtcccgagg tcggtctcgg aggcgagata gagcgtcttg
    36241 gccatcgcct cgatccgctc ggcccgggac cgcacctgag cggcggactc ctcaccactg
    36301 acatacagca cgtcgcgccc gccgcgcgcg gcccgggcgg cgacgtcgag gagcagggtc
    36361 gacttgccga tgccgggttc gccggcgacg aggacgacgc cgccggcgac cagcccaccc
    36421 ccgagcaccc ggtcgaactc ctccaccccg gtcggcgtgg cggccgcctt ggtgggatcg
    36481 acctcgccga tcggcaccgg ccgccggccc gggctgagca ccgcggccgt gcgggtcgtc
    36541 gccccgccgg cctcggccac cgtgccccag gcctggcact cgccgcaccg gccgacccac
    36601 ttcacgctgc tccagccgca ctcggtgcag cggaaggagt tcgcccgcgt cgtcttcgcc
    36661 atgacgcaga cgctagacgg cggcaccgac aacccgggcc gggcccgcct cagccgggta
    36721 gcgacgggtt cggcggctgc gcggggatcg acggatcgga cggtatggcc gggtcacccg
    36781 gtatggccgg cgtgctcggc tcgctcggga aggacgggtc cggcggcgag gccggcggat
    36841 tcggtgccgg gaacgccgga tcagtgggtt gggtcggttc agtgggtgga gtcggatcag
    36901 tgggctgagc cggctcgctg ggctggatcg gttcagtggg ctgggccggt tcagccggcg
    36961 gcactggctc gctgggctgg gctggctcgc cgggctgggt cggttcagtg ggcggcgccg
    37021 gctcgctggg cggcgccggc tgggcggggg tggtgggctc ggcgggaggc gcaggagtgg
    37081 gagggggcgc cggtccaccg ggcggtgcgc cgatgtcgtc gttctgtccg tcgaggccgt
    37141 cgagggcgtc cttggccttc tccccggctg cgtggatctg ctcgctgtac ttggaaccgg
    37201 tgcgctcgtc gatggcggcc gcggccctgt cgaccatctc gccgatctgg tcgccgaatt
    37261 tgtcgacggc gccggtggcc atgtccttgg cctgttcgct gctgaagcgc tgcgggtctt
    37321 gcgtcattgc ggggctcctc cctcgccgga acgtcggacc acagcaggtt ggcacgacat
    37381 cggcccgggc gggggcacag cagccaccgc tcgcccaact cagcagaggt tcctcagccc
    37441 accccgccac gtagactgtc gccgcccggc atcaggggtc gacggaggga cacgcgtggc
    37501 gcagcagtcg gaggaggagc tctaccgctc gaccctcgcc gtgctcgcac ccggcaccga
    37561 actgcgcgac gggctggagc ggatcctgcg cggcaacacc ggcgccctga tcgtcctcgg
    37621 ccacgaccgc acggtcgagc agctgtgcac gggcggcttc cccctcgaca tcgagttctc
    37681 ggcgacccgg ctccgagagt tggccaagat ggacggcgcc atcgtcgtgg actccgacct
    37741 gtcccggatc gtgcgcgccg ccacccagct cgtgcccgat ccgagcatcg agacgatgga
    37801 gtcgggcacc cggcatcgca ccgccgagcg ggtcgccaaa cagaccggca tgccggtggt
    37861 ctccgtcagc cagtcgatgc ggatcgtgca gctgtatgtc ggcagccgcc gccacgtcct
    37921 cgaaggctcc agcgagctgc tcagccacgc caaccaggcg ctggccaccc tggagcggta
    37981 ccggtcccgg ctcgacgagg tcacggcgac cttgtccgcg ctagagatcg aggacctggt
    38041 caccgtccgc gatgtcgccg ccgtgctgca acggctggag atggtgcgcc gcatcagcgg
    38101 cgaggtcagc ggttacgtcc tcgaactcgg tgtcgacggg cgactgctga ccctgcaact
    38161 ggaggagttg accaccggcc tgaccgacga cctcgagtgg ctgatccgcg actatctcgc
    38221 cgacggggtc gtcggcgatg acctcatccg ggaactcatc gacggcctga ccgcgctgag
    38281 ccgcaccgac ctgctcgatc ccgcctccgc cgcccgggtc atcgggctcg gtggccccga
    38341 gggcctcgat tcgagcatct ctccgcgcgg ctaccgcctc ctcaacaagg tcccccgcct
    38401 gccgggtgcc gtgttcgaac ggctcgtcga gcatttcggc accctgcaac gcctgctcgc
    38461 ggccacccag gacgacctca tgctcgtcga cggcgtcggc gaaggccggg ctcgcaccat
    38521 ccgcgaaggc ctgagccggc tggcggagtc gagcatcctg gagcggtaca tctgaacgcg
    38581 cgcgagcgca cgcgcgtgca ccgcgccgtg ctgcagtggt atgccgtggc cgggcgcgat
    38641 ctgccctggc gcgaaccgtc gtgcagcccg tggggggtgt tcgtcagcga ggtcatggcg
    38701 cagcagacgc ccatcgcccg catcctgccg gcctggcagg cgtggctgcg ccgctggccc
    38761 acccccgccg acctggcggc agccccggtc ggcgaggcca tccgcgcgtg ggaccggctg
    38821 ggctacccgc gccgggcggt caacctgcac gccgccgccc gcgccatggt cgagcggcac
    38881 gacggacacg ttccgtccga cccggaggag ctacgcgccc tgcccggcgt cggcgactac
    38941 acggccgcgg ccgtttccag cttcgccttc ggcaacccgc tcgtcgtcat cgacaccaac
    39001 gtccggcggg tgctcgtgcg catcgccctc gggcaggcgc aggccgcgcc gagcctgacc
    39061 aaggccgaac gcgagctggc ggccacctgg cagccgcggg cccgcagcga cgccaacgcc
    39121 tggaatgtgg cggcgatgga actcggcgcc ctcgtctgca ccgcccgggc cccgcgctgc
    39181 tccgactgcc ccgtcgccgc gctgtgcgca tggcaccagg ccggcaaccc ggcatacgac
    39241 ggaccggccc gcggcggtca gggttacgag ggcacggacc ggcagctgcg cggccggatc
    39301 ctggcgctgc tgcgcgcggc cgaccacccg ctcgagccgg ccctgatcga ggcgttggac
    39361 cccgacccgg aacgcgtgcg gcgactcgtg ggagcgctgg cccgagaggg gttactcgag
    39421 cacagccgat ccggctacca tctcccgcgg tgacctgcag aggttcacct ctacgtcgtc
    39481 tccccgtcaa ccggatgccg gtctggggcg tctgacctat gacggccgca gacggcggcc
    39541 gtccacccac gtcccgaggg ggcccgcttg ccggcgacac cgagccgccc cgcaccgacc
    39601 cggccggcgg gcgcgctgcg gcataccgcg ggcctgacca tcctcggcac cctgatcccc
    39661 ggcgccggcc tgctgcgcac ccgagcgcgc aacctggggc ggctcctgct cctgatcgcc
    39721 gggctcgtgc tggccgcctt ggtctacaag gtgctgcgcg acggcgtcgt cgggtccgcg
    39781 ctgcaggtcg gcgtgtcccg gcagaccatg ctcgtgctca tctgtgcggt cctgctcgga
    39841 gcgctcgtgt ggatggggtc gatcgtcttg acctcgatcc ttgcgcgccc gcgcggcatg
    39901 acgcgcgacg aacggctcgc cagctcgatc gtcgccttct tgtgctgcct cgcggtggcg
    39961 gcaccgacgg cgtatgcggt gcgcaccctc ggggtgcagc gctcgctcgt cgccgaggtc
    40021 ttcggcagcg aggacggcag ccccggggcc ggcggcaccg gcacgcccga cccgggcgcg
    40081 gacaacccgt gggccgacat cccccgggtc aacgtgctgc ttctcggctc cgacgccggc
    40141 accgaccggg tcggggtgcg caccgactcg atgatggtcg ccagcatcaa cacccgcacc
    40201 ggcgatacgg tcctgatcgg cgtcccccgc aacctggaga atgcgccgat ccccaagagc
    40261 aacccgctct cgacgctcta tcccgacggc tacaactgcg gcgacgaatg cctcctcaac
    40321 ggcatctgga ccctcgccgg cgaccacgcc gacctcttcc ccggcgagac caacccgggc
    40381 ctgcgcacca ttcgcgaggt gatcggcgag atcctcggcc tgaagatcga caacaccgcc
    40441 gtcatcgacc tggccggatt cgccgacctg gtcaacgcga tgggcggggt cgacatcaac
    40501 gtcaaggagc gggtctgcat cggctgcaag gccctctccg acggcaccgt cgtcgggacg
    40561 acggactgga tcgagaccgg tccgcagcat ctcgacggct accacgccct gtggtacgcc
    40621 cgttcgcgcg cgaccaccga cgacttctcc cggatgcgcc ggcaacgctg cgtcatgggc
    40681 gccctgatca aacaggtcag cccgagcacg atgctcctgc gctatccggc actcgccgaa
    40741 gtcctcaaga cccacgtgag tgtcgacatc ccccaatcgc agctgcaggc ctgggtgcct
    40801 ctcatcgagc tggtgcaggg caagggcacg ctgcgcagtc tcccgctgac caacaagctc
    40861 gtcgacgtcg gcaaccccga ttaccccgcg atccgcgagc tcatcgccga cgcgacctcg
    40921 gacaaccccg tcacctcctc gaccacggcg tccagcacca cgtcgccggg gtccgccgag
    40981 cccaccggag atcccaccac ctcgaccggc accgagacga ccaccgagcc ggccgacggc
    41041 ctcagcgaca tctccaccac gtgctgatca cgtgcgctgg atcgtcaccg tccggtgctg
    41101 gacaatgctg acgagctcga cgccggatcc cccgccctgg cgggcctccc ggctccggct
    41161 ttcgtcggcg gcagcaccga ggaagcgata ggtccactcc tgtgccagcg cccggttctc
    41221 ggcgaaaacg attggcacgg tggcaaattg cacctgcagt tcggcgagca tgtcggccac
    41281 catggccggg cggatgtgcg tgagtttgaa gatcgacgag tagcgatcct cgacgacgac
    41341 ggcggcatgc gggagcgaca ccagttcgac aacagacccc gcagcgtgcc gctgctgagg
    41401 ctgcccgcga ggtcgtcgag cgtcttgcgt tcgacggcgg cgacgatgtg cccgttgacc
    41461 tcgatggcgt agtcgcccag cggcagtgcc cgcttcgtgg tcgtggcctg ctggtgggtg
    41521 aacttccagg actagcgctc gcgggtgtcg acgatgatcg cgatatcgac gattccggcc
    41581 gccctgctcg tcggcaacga cacgttgggc cgtgccgtct tcgaggtcct cggtgactgc
    41641 cagaagatca tctctcggcc ccgggcccgc gtgatcacga actgcgagcg gctctcccgg
    41701 gcgcgagcga cgacgatgtc gatcgccgca ccccggggca cacatgaggt caggggcaac
    41761 tcctccaaga cctccgcatc gtccggccac ggcgctccgt cccggtggca atacactttc
    41821 gccgtccgtg gccacgtgtc ccgcgcccgc agcacgatcc cgtccccgag cgggatacga
    41881 agcagatagg gcaacgtcga gtccggctcg gggtccgcgc gatcagcagc gtccccgcca
    41941 ccaccccagc atacggaccc gtgagaaccc gcccccgccc cggcccctcc gtcgagtgaa
    42001 caccgataac gcctcgagtg aacaagttgt ccacaggagg ggcgatttgc ggtggccgga
    42061 ttctgtgatg acgcgcagca ttcgggggca tgagcgacct tgagataacc gactcgcccc
    42121 tgaccaagcc cggcttttcg cccccgccac cggatgatct gggcatcctt caacagatcg
    42181 cggcgtcgat cagcatgcct ttcgccttct cccactgc
//
LOCUS       DAOPJG010000007        40619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1022_length_40619_cov_7.840893, whole genome shotgun sequence.
ACCESSION   DAOPJG010000007 DAOPJG010000000
VERSION     DAOPJG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 40619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40619
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1022_length_40619_cov_7.840893"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKU75_01555"
     CDS             <1..402
                     /locus_tag="PKU75_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPF79590.1"
                     /translation="GLAALLELRLGESGKQALGFAVHVPHYLAQADFHDGALSALNAI
                     VDATGLNLPNDALVQAAAEGRAQIDAEVAGNPEIAGAVNALERQYDSFIEAADKPNLL
                     TPQANPIPSADELAADVEEFLKSVSDEPDAG"
     gene            complement(428..2077)
                     /locus_tag="PKU75_01560"
     CDS             complement(428..2077)
                     /locus_tag="PKU75_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPF79591.1"
                     /translation="MPSPKSLVALVAGPAGFAVTDGRQPRAFGPEQVAEVAAALADAP
                     RVVVWSAARDLGALVRAGTPPPRVLDLAECHRLERGRWRADPGRVWARLHGLAGDGIP
                     DLAPDDLFSALAEPVPPEALSTPAGYLRPDAVAGTWQTTPQRLHAWAAAAYDCGKRRW
                     DALGRNEVWRATAVSESSAAVLCLELERDGLPIDRPGMEALIARSAGSRPRDAADGLR
                     IRADRDARVLAHTPGHDGVDLRNPGAVKALLAAIGIDVPSTRKWLLEPYREAHPLVPA
                     LLEWRAQERIATTYGYHWLDTHVGPDDRLRGGWTACDGAAGRMTAENGLHNLPAVLRP
                     AVRAAPGHLFVRADLGQIEPRVLACVAQDESFAAATASDDLYAPVAERLGVERARAKV
                     AVLAAMYGQRSGAAGEALAGLQRAYPVAMGFLDRAYEAGVAARDLRTYGGRRIRTAGS
                     LDPGSRGRYARNALIQGAAAELFKAWASTVRVTTADLGAQVVLCLHDELLVHVPAEQA
                     AECAARVEQALTDAARRWSGGAPVRFVTDTSIVERWSQAKG"
     gene            2238..2975
                     /locus_tag="PKU75_01565"
     CDS             2238..2975
                     /locus_tag="PKU75_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HPF79592.1"
                     /translation="MGLFGFGKDDDVKEMEKDISVTGSALERAKKGDEGGMAGAATRL
                     MERLLDTGIDGRGPLDSAHKIATDALAKRRTTDEAVDEIVKDHTMMAATGGFLTSLGG
                     FITMPVAMPANIVEFYLLATRMVASIAKTRGYDIDKPEIRSAILLTLVGADADDLLKK
                     AGVVATGRLSNLAAQRLPGPILMVVNKAVAFRLLASAGRNTLARFGKGLPVAGGFVGA
                     GLDTYLIRKIADSARREFPPVTDRTVR"
     gene            3057..3485
                     /locus_tag="PKU75_01570"
     CDS             3057..3485
                     /locus_tag="PKU75_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HPF79593.1"
                     /translation="MAARSRLLLARSLMSAVRSATRPGAPSMAERAGSVPRLVRAVAA
                     GDYRGMTVGRLGMLAVAAAYVISPVDLLPEGFLAVFGLADDAMVLSWLAANLVTQTEE
                     FLEWERAGTPGSNATAADPQDAATSAYAGAGATVSGRVVR"
     gene            3588..4694
                     /locus_tag="PKU75_01575"
     CDS             3588..4694
                     /locus_tag="PKU75_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 family protein"
                     /protein_id="HPF79594.1"
                     /translation="MTVHMTSPGEFLTSLPYQIGFYPHRSLVVVMTRDKTVILVARMD
                     VPEHPVHADRALDAGCADLQRQIGAGNLDGAHLALFDDSLLGLAALVRVGDRLAESGV
                     TLGLAALVSDGMFRLLGAPDGNWDTLPRPEDCAIVAELVAQGRSVAPDRDAAIACLAP
                     CRPPTTAGTELTARECSVPVHAIGEWRALLALVPPDEPDPWQPSPATAIRLSRSLLDR
                     DFRDAMVAGLVPGTLPDSELVEPAATRAARILAPLHDELDRHRTARRHVEGGEQPPWH
                     GPDLQRARLREVLERILTVARLAPAPIAAGPYAVAAILAGRLGEGLVCTGAIEHALEA
                     DPDHVLANIMADMVAAGHVPGRPPGTVVQDSGAA"
     gene            4874..5263
                     /locus_tag="PKU75_01580"
     CDS             4874..5263
                     /locus_tag="PKU75_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPF79595.1"
                     /translation="MAIVVGYVPTKEGRAALTRAAEECKLRGTSLVVVNSARGGRDMV
                     DPEQRDGLDNLASDLKADGVEVEVRSPVRGLEPAEDLIAIAEEINADFIVIGLRRRTP
                     VGKLILGSNAQRILLDSPCPVLAVKAD"
     gene            5553..7262
                     /locus_tag="PKU75_01585"
     CDS             5553..7262
                     /locus_tag="PKU75_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPF79596.1"
                     /translation="MPRSAAPAPELPREFSHTALQDLVRLGHTAGAISANQLVAAMTA
                     ASVTERRRKAVVRAIEEQGIEVVLDMPTTAKTTKAAKAPAKSAAKSAPTKRAAKNEPA
                     DEAVETTSDATSAAKPARKTAAKAAAKAPAKTAAKAAAKTTTAKTTAKKASAKTATET
                     TATEKTATEKTAPGDAADVAEQPEGEEALADDAEPEDLEDAEEPDVEADAEAEAEAPE
                     SADTQDGEEADGEAAPAAAGEDDQDESAGFVIRDDDEDDAPAQQVVTAGATADPVKDY
                     LKQIGKVALLNAEQEVELAKRIEAGLFAEERLNSGEIADQKLKRELWWIAQDGKRAKN
                     HLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWI
                     RQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELARELDMTPEK
                     VVEVQKYGREPISLHTPLGEDGDSEFGDLIEDSEAVVPADAVSFTLLQEQLHSVLDTL
                     SEREAGVVAMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSKLRHPSRSQVLRD
                     YLD"
     gene            complement(7323..9701)
                     /locus_tag="PKU75_01590"
     CDS             complement(7323..9701)
                     /locus_tag="PKU75_01590"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HPF79597.1"
                     /translation="MRGQARWSDALLAAAASWAAMLPIQTLLRSLAWVNPALLITAIV
                     VVTGVLARTVLRNWLAVAAVQLLVGVESIVLMYARDETWYGLPHWGTVLALNEILVEA
                     RETIQAYAAPAPTTPGIVLTLSLLTLVVVLAVDALAVSRRSPGAAGLALLSAYLVGAA
                     NSSSTLRWIHFLLPAALWLLMLARAGTVGLRRWASVLPRTPHTQARPADPVRSFARTA
                     GVLGSVVAIGAIVLAAVVPHFPTRYLADGLGRGTIDRADGTVTLSTTLDLRASLENPS
                     SAPLLRYRTSVGSPSPLRVAVLEDYRPDGQFRQRPQYPARNVSKGEPLQQQEAISSTW
                     VRYLDDLPEEAKRAGTLSATGSRLAKPQIAIPYGATRLTMPEGVEAIEFGEGTVQVLS
                     RAESYDVEYIDIAPTPELLEQSESYVGPDPWAETVEDDSLLVEERARPAIAEALADAV
                     PEGANRFETAVAIQDWLRSPVFTYDTQLAPLPEGSDVDPITHFLQTRVGYCQQFATAM
                     IMMARERGIPARMVIGYLPGRLDRGEWEVRASDAHAWPELYFPPFGWIRFDPTPGSRS
                     GAAPSWANLPESTATTWSASTSASATRSSTRPVNPGQDDPGTQDVTPADRTLRQRIAA
                     ISARTWILLALGAGVLSMLVLPVTSMLARRRRIAAADDDSDVIEARWQDLVARIGDLG
                     IKTPPTLTPRQVHSHLTQAAVLDQDTASALRRVVSAVEVARYAAPGTPVADPQPDVDA
                     VFESVDASRSPSVRLRARLLPYAGRAAVRSLVQRVTNLPARLGDELRHRGDE"
     gene            complement(9712..11013)
                     /locus_tag="PKU75_01595"
     CDS             complement(9712..11013)
                     /locus_tag="PKU75_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPF79598.1"
                     /translation="MSALGGRSGIVRGSTAPRRRRRRLTDALTLRGRCFLAAGVALMV
                     CGLLLGFRDITRIGFLVAGLPLLMLLLARRQRLSMRTTRTVTPSIVPIDQWSDVNLRV
                     ENPGPATTPILMAEEQLDYALGDRPRFVVPRMAPGAEHLINYRVRSTVRGRHRLGPLG
                     LRVKDPFDMTLRLAVVRGTGDLIVLPRIVSLSGTTVAGGLGNEGTIPHMIALHGEDDV
                     SVREHREGDDLRRIHWPATARTGDLMVRQEDRPATRRIVLLLDDRATVERAPRGTASF
                     EWAVTAAASIANEVIGSGTNTFLALGGEIRSGHGGEHLRVEEALTELAVAELREPVQF
                     VTTMALAADAAASGALCIAVVAAMAERDTERLARVRPPGSPGMALVLPVSDSDDPVAA
                     SATATGLRERGWSAVVITPGTSLVDAWAAAAASPALQGGRR"
     gene            complement(11025..11939)
                     /locus_tag="PKU75_01600"
     CDS             complement(11025..11939)
                     /locus_tag="PKU75_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPF79599.1"
                     /translation="MANAMKTVIEGKPEVIQTALTALLAEGHLLLEDVPGVGKTMLAK
                     TLARSIDCTVRRIQFTPDLLPSDVTGVNMFNQDMRAFEFRPGAIFANVVVGDEINRAS
                     PKTQSALLECMEEGQVTVDGRTYTLPHPFIVMATQNPIEMEGTYPLPEAQRDRFMARV
                     SLGYPSSRSEMDMLESHGERDPLEELRPVTDAATVSGLIKAVRTVHTSDAIRQYIVDL
                     AAATRDNTSVRLGASPRATLHLLRATRAHAALAGRDYVLPDDVQDLVVPVWGHRLILT
                     GEAIHARRSPADVLRGLTERVRVPAPGF"
     gene            12341..12781
                     /gene="mraZ"
                     /locus_tag="PKU75_01605"
     CDS             12341..12781
                     /gene="mraZ"
                     /locus_tag="PKU75_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPF79600.1"
                     /translation="MDVEFLGHHSPRLDEKGRLFLPAKFRDDLGDKIVVTKGFERCLT
                     IYPHPVFKQRILDPLNAANTSSKTVRSVRRQLLGDASTENPDRQGRVTIPGVLRSYAG
                     LDKDCVVIGQGHYLEVWDSATYEAANATDGTTLEDLHDEGVVLL"
     gene            12937..13917
                     /gene="rsmH"
                     /locus_tag="PKU75_01610"
     CDS             12937..13917
                     /gene="rsmH"
                     /locus_tag="PKU75_01610"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPF79601.1"
                     /translation="MNRSAPADHRHVPVLLERVVEVLAPAVREPGSVVVDATLGLGGH
                     AQALLDACPEATLVGIDRDAQARDAAGERLAAYGDRVRIVDAVYDELPEVLAGLGIGK
                     VLGILFDLGVSSLQLDAAERGFAYRFDGPLDMRMDPSRGASAAQVLRDYDAAGLTRVL
                     RDYGEERYAAKIARAIVAERERTPFETSGQLVELLTRVIPAAAQRSGGHPAKRTFQAL
                     RIEVNDELRVWERALPAALDALAVGGRIVVLSYHSLEDRITKTNLAAGARSSAPVGLP
                     VELPEHRPYLKLLTRGAEEAGAAEQATNSRSASVRMRAAERIRDTSKGSR"
     gene            13914..14396
                     /locus_tag="PKU75_01615"
     CDS             13914..14396
                     /locus_tag="PKU75_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79602.1"
                     /translation="MSSTALKLSGAPAKAPGRPPLSVVRGGAGVRQAWFSLAMLAVLV
                     LGLLATLVINTALAEGSYERGKLVTESTVLADRQESLTTDLDNLRAPAALAKEAMSMG
                     MVPAQSAAFVRLADGAILGVAEAATADKRFTVITSPTLPKSTAKSSAAAPSSAPTSKD
                     "
     gene            14544..16478
                     /locus_tag="PKU75_01620"
     CDS             14544..16478
                     /locus_tag="PKU75_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPF79603.1"
                     /translation="MADRTEGPRAPRPAARAPRPDRAPRPDRTTRPQRTARGARPLAR
                     PGANPRVRARGILIGLLVVLSLFAAQLVRVQGLDAGAAAALAMQTRVYTETTPALRGT
                     IYDATGTILASSIEKRIVIADPKAVVEYKKKVDGKTTKVGAAGAAADLAPLLKMSEAD
                     VLKEITRPKTRYVEVNKNVSPLTWHTIAELGIPGIYSKRTSVRTYPQGSTAASLVGFT
                     GENQEGAGGIETYLNSSLRGVPGELKYEVGQDGIRLPMGASTESPAKTGRSVQLTIRN
                     DLQWYAQNVLAQKMIDVRASSGTVVVTNAKTGDLLAIASYPTFDPNTKIADNAQLTNL
                     AFTEVFEPGSTAKVITAAAALEEGKVTPSTPMIVPYSLRRSDGQISDSHSHPTEYLTF
                     AGALAQSSNTGIVLAGEQIPPATMESYFRKFGFGSKSAVNFPGESAGLLAPAGQWSGT
                     QRYTVMYGQGLSVTALQAAGVYQTIANGGVHIAPKLVTAMADANGKLQPTPESAKTRV
                     ISQDSARQLSQMLEGVVGADGTAPAAQIPGYRVAGKTGTALRYDSRVKGYAGYTASFI
                     GYAPAGDPELVVSVIVQNPKKEYYGGSVAAPVFHDVMLYALTSLDIPPTPGDTEPPKL
                     TLKLDETPDPDDPAVLRDRR"
     gene            16489..18021
                     /locus_tag="PKU75_01625"
     CDS             16489..18021
                     /locus_tag="PKU75_01625"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPF79604.1"
                     /translation="MIDIRPERRAPVPLARVAAVAGATGPTADAAAVTVTGVGLDSRT
                     IRPGDLYAALPGANVHGGRFVDGAVERGAVAVLTDSAGAALAAGTPVPVLIVADPRAA
                     LGQVAAEIYGTRPPGLDLIGVTGTNGKTTTAYLIDSALRALGRRTGLIGTVETRIGDQ
                     RVKSVRTTPEATDLHALLAAMREADVATCVMEVSSHALAMHRVDGVVYDVALFTNLSQ
                     DHLDYHGTMEDYFLAKAELFTPERATRGVVCVDDAWGARLAGMATIPVSTVSSTGAPA
                     DWRLDAAPDGAEFTLTGPHTQLALRSALPGDFNRVNTALATLALLALGHDAGAVREAV
                     LTDPHVPGRMEKVAPAAGSDAPLAVVDYAHTPEAVAAALAALRPQAVPGGLVVVLGAG
                     GDRDRGKRAAMGAAAAREADIVIVTDDNPRSEDPAVIRAAVVAGARESAPHKDIREIA
                     DRAEAIAAAVAAAVAAGRGAVVAVVGKGHETGQEIGGVVHPFDDREQVRAALDAAYGQ
                     AS"
     gene            18018..19430
                     /gene="murF"
                     /locus_tag="PKU75_01630"
     CDS             18018..19430
                     /gene="murF"
                     /locus_tag="PKU75_01630"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPF79605.1"
                     /translation="MIPTTLTELAEVTGGRIAGLGESEAGGLTITGAVVTDSREAEPG
                     SLYVARIGEHADGHGFIAGAAQRGAVAALVTQDTGPLPRVVVADEQAAFVALARHLVD
                     ARPQLNVVGITGSSGKTSTKDLLGQVLARDGETVAPVGSYNSEVGVPLTVCRITDSTR
                     HLVVEMGARGIGHVRYLTEIAPPRIGIVLNVGSAHVGEFGSQAAIAVAKSELVQALPS
                     DGVAILNADDPLVRAMAGRTTARVLLVGEAQDAEVRATGVTLGADGRARFRCSGPFGT
                     AEVHLAVAGRHQVGNALAVIAAAHESGMAIETIGAALRDAGPVSRYRMEVATRADGVT
                     IVNDAYNANPESMTAALDAVAAMAGGRRAVAVLGPMLELGAEAEAAHRAVGSAAAVHG
                     VHTVIVVGDQARGIGEGASSGGSHVEYAPDVESAYAMTVDIVEAGDIVLVKSSNGAGL
                     RFLGDRLRDTPVSAKEDLTC"
     gene            19424..20530
                     /gene="mraY"
                     /locus_tag="PKU75_01635"
     CDS             19424..20530
                     /gene="mraY"
                     /locus_tag="PKU75_01635"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPF79606.1"
                     /translation="MLAVLVSAVVALVIGLLGTPAYIKFLVKRGYGQFIRDDGPTSHH
                     TKRGTPTMGGTVIIAATVVGYFVAHFSTAVIGSTGYAAPTASGVLVLFLMAGLGLVGF
                     LDDYIKISRQRSLGLRSKQKLAGQALVAIIFAVLALRFPDGNGTHPASTAISFVRDTK
                     LDLAFGGTLVGIALFVIWANVMISGTSNGVNLTDGLDGLATGASTMVFAAYVLIGVWQ
                     SNQNCATSLNINNCYAVRDPLDLAVVAAGGMGACFGFLWWNAAPAKIFMGDTGSLALG
                     GALAGLAITSRTELLVIILGGLFVTITLSVIIQVASFKSTGKRVFRMAPLQHHFELKG
                     WQEVTIVIRFWIIAGLFVAFGLGIFYAEWVVGAL"
     gene            20502..22004
                     /gene="murD"
                     /locus_tag="PKU75_01640"
     CDS             20502..22004
                     /gene="murD"
                     /locus_tag="PKU75_01640"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPF79607.1"
                     /translation="MRSGSSVPSDRLQGLTHGGADWSGLRVLVAGLGISGFAAADALA
                     ERGARVVLVDGQTPAAGSELAQRATILQVLDVEVRTGPQATAALPADLDLDVIVTSPG
                     WRPDQPLLAAAAAAGIPVWGEVELAWRMRPPEGAAPWLTLTGTNGKTTTVQMLASILR
                     AAGLRATSAGNVGTPILEAVLHPEPYDVIAVELSSFQLHWSESISPLASACLNIAPDH
                     IDWHGSFEEYWRAKAKVYARTEVACIYNVADDETRRMVEEAEVVEGARAIGFTLGIPS
                     VSMVGLVEDVLADRAFVAERATSAAEMGTLADLSQGDVPPPPHVVANALAAAALARAY
                     GVPTGAVRDGLRAFVPEPHRVAEVATVGGVRFVDDSKATNPHAAAASLAAFPSVVWIA
                     GGLLKGADVDELVAAHAARLRGVVLIGADRARIGESLARHAPDLPVVDLSDTDTGAMD
                     RAVAAAADLAQPGDVVLLAPAAASMDMFANYGARGDAFADAVRRLPTTGE"
     gene            22008..23282
                     /gene="ftsW"
                     /locus_tag="PKU75_01645"
     CDS             22008..23282
                     /gene="ftsW"
                     /locus_tag="PKU75_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPF79608.1"
                     /translation="MSSTTASPLSSRAAGRRRLARLRELLDSPVATYYLLLSVTTLLT
                     VIGLVMVLSASMIVSIKDSDNPYAVFYTQAIFAAIGTVAMLIAMRLPLGFWKRIGGAA
                     LIGAIGLQALVFSPLGFDVQGNRNWIKLPGFSLQPSELAKIALILFGARVLTQKRRFL
                     GSFKESVFPFVFPAAAITLGFVMLGHDLGTAIVLAAIVGGMLLAGGVPLRWFGLAGLG
                     GLALVAAATMTSSNRMQRISIWLDPSQCVPSNERLYYDICRQPLHARYALADGGLTGI
                     GLGASKEKWQWLSEPHNDFIFAIIGEELGLIGALIVLALFALLGYAAYRLVVRTDDFF
                     VRIAASGVMVWILVQAIVNIGAVIGALPIIGVPLPFVSSGGSSLITTMLAMGILLAFA
                     RAEPGCAESLSVRPKTVRDSMAVLSRTRGKQR"
     gene            23279..24421
                     /locus_tag="PKU75_01650"
     CDS             23279..24421
                     /locus_tag="PKU75_01650"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPF79609.1"
                     /translation="MTDAGPAATTPASWPAAVLLAGGGSAGHVSPLLALADKLQQLRP
                     DMGIAALGTETGLEARLVPARGYPLHMAPKVPLPRRPGPDLLRVPGRLAGAIKAAEAA
                     IDATGAEVVVGFGGYVSTPAYLAARRRKLPIVIHEQNARPGLANRLGARMTPFVGTTF
                     ASTKLAHARRVGMPLRPEISTLDRPARRAEALQRFGLADHRPTVLVTGGSLGAKRLNE
                     AFAARVLELSEAGIQVLHVTGNGKAFAAPDTGAGARYVVLEYADAMELGYAAADLVVA
                     RSGANTVCELSAVGLPAVYVPLPIGNGEQELNAADVVAAGGGLLVPDAELTPTWVTGT
                     LIPLATDAGRLQSMSAAAAAVGERGGDQLLADLVAQAYAAGRAGGH"
     gene            24421..25887
                     /gene="murC"
                     /locus_tag="PKU75_01655"
     CDS             24421..25887
                     /gene="murC"
                     /locus_tag="PKU75_01655"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPF79610.1"
                     /translation="MAPGRLGVNPRFDFAAPTTPASELGATHFVAIGGSGMSGIAQLL
                     HDLGVEVSGCDGAASPAVERLRAAGMAVSIGHDPGHLDGVRTVVVSSAIPETNAELTA
                     ARAGGLRVLHRAQALASAMGDDVRVAVAGANGKTTTSAMLTAVLRAAGADPSFAIGSE
                     LADLGRNSLRGTGGVFVAEADESDGSFLSYRPHVAVVTNVQPDHLDFYGDFAHVQQAY
                     AAFARSVEPGGWLIAAADDPGSAALARLLRSEGRQVLTYGEDPASDVRIEEVELHGLT
                     STARVVSDRGAVPLRVAAPGRHNVHNAAAALAAGWLALGRDPAQLTAGLAAFPGVHRR
                     FERKGEVDGIVVVDDYAHNAPKVAALVAAARAVVGAGGAVRVIFQPHLYSRTRDFAAG
                     FAAGLQPADQIVLLPVYGARETPMAGIDSHLIVEPLRDAGADATLAAGFDEAIDLVAS
                     RAGPGDLLLTVGAGDVTRIGPRLLAALAARSHAGPDRA"
     gene            25880..26647
                     /locus_tag="PKU75_01660"
     CDS             25880..26647
                     /locus_tag="PKU75_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPF79611.1"
                     /translation="MRETPPSTLESERTEQRRVLSFADGWKRRARALRRRRAGKVVWL
                     IGAAVLVLVLAWLALWSPLLAVTEVQVSGTTPAVRSLGKSVGGQEVGRSLLRVDTAGV
                     EGRLEEDSRVADATVRRGFPRSLVIEVVARVPVLGLQKSQGKVELVDVSGVTIETVSE
                     APAGVPVVQGATGVASGDGVHATVQVMAALPESLRTRVRDLHIDDSGSVSFVISATRI
                     VWGPAEESELKARLVQILMSKKPKTIDVSAPETPVTT"
     gene            26924..28174
                     /gene="ftsZ"
                     /locus_tag="PKU75_01665"
     CDS             26924..28174
                     /gene="ftsZ"
                     /locus_tag="PKU75_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPF79612.1"
                     /translation="MAAPQNYLAVIKVVGIGGGGVNAINRMIEAGLKGVEFIAINTDA
                     QALLMSDADVKLDVGRDLTRGLGAGADPEVGRRAAEDHAEEIEEVLKGADMVFVTAGE
                     GGGTGTGGAPVVAKIAKGLGALTIGVVTRPFTFEGRRRANQAETGIGSLREEADTLIV
                     IPNDRLLSISDRNVSMLDAFRSADQVLLSGVQGITDLITTPGLINLDFADVKSVMSGA
                     GSALMGIGSSRGEERAVQAAEAAISSPLLEASIDGAHGVLLSVQGGSDLGLFEINEAA
                     RLVQEAAHPDANIIFGAVIDDALGDEVRVTVIAAGFDGGTPQKRTDERALGTVVSRPG
                     VPAPAAGARPAPAAPAESAGQAAPAPTPAASGAPAAEPADAPAAEPAPAQTAQPAPAA
                     EPVRPPKQVVFDDPEDLDVPEWLK"
     gene            28191..28949
                     /gene="pgeF"
                     /locus_tag="PKU75_01670"
     CDS             28191..28949
                     /gene="pgeF"
                     /locus_tag="PKU75_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPF79613.1"
                     /translation="MFAWLDEGSRVVRGFTDRHDGHSAGAHAGLNLGSRVADDPAAVA
                     RNRAAAAAALGVPAGNLVFLHQVHGAQVATVTEPWGPEDEPEADALVTTVPGLALVVL
                     AADCVPVLLADPVEGVIGAAHVGRPGLLAGTVAAVVERMRSLGAVDLDAAVGPAVCGS
                     CYEVPQQMHDDAVALVPAARAVSATGTPALDIATGVLAQLSAAGVSARQVLGCTRESG
                     SLYSYRRDGVTGRHAGIILLRPPAAHRPGRHHPG"
     gene            29118..29852
                     /locus_tag="PKU75_01675"
     CDS             29118..29852
                     /locus_tag="PKU75_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPF79614.1"
                     /translation="MSAARADAVAARPERVEEIRAGLAAVEERITAACAAAGRAREEV
                     TLIVVTKTFPASDIRILAGLGVRDVGENKVQEAHAKAEAASYDGLRLHLIGQLQRNKA
                     AEAARLADTVHSLDRPKLVAALAHGRERAGLAEPLRVLIQISLDGDPERGGAPRDTIP
                     ALAEAVLAQPLLRLGGVMAVPPLAADPAAAFADLAGLAAAVRRSAPEADWISAGMSAD
                     LEAAVAAGATHLRVGTAILGSRASLR"
     gene            29930..30442
                     /gene="sepF"
                     /locus_tag="PKU75_01680"
     CDS             29930..30442
                     /gene="sepF"
                     /locus_tag="PKU75_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPF79615.1"
                     /translation="MAGALKKTMVYLGFAEDDRYDDYYDAEAYDDEPTEAAPARSERH
                     EVRQDVRPERSAEVTPMPRRATLSQVVREERASTLSRITTIHPRTYNEAKNIGEAFRE
                     GVPVIMNLSDMGDADAKRLVDFAAGLVFGLHGSIERVTAKVFLLSPEHVEVATDESAI
                     EGPRELFNQS"
     gene            30535..30810
                     /locus_tag="PKU75_01685"
     CDS             30535..30810
                     /locus_tag="PKU75_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPF79616.1"
                     /translation="MSLVREILSTALFLYFLLLIGRLVFEWIQVFARDWRPRGPVLVV
                     AEGIYTATDPPLKALRRVLPTLRFGNVGIDLSFLVLMIGISVLMSIL"
     gene            30864..32096
                     /locus_tag="PKU75_01690"
     CDS             30864..32096
                     /locus_tag="PKU75_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPF79617.1"
                     /translation="MPLTPDDVLNKTFATTQFRRGYDEREVDDFLDEIVATLRRQEKE
                     LRECRGSEPVQDAASAVPVMDASDGRLEREVALREEAERALADLQKRMRKGDEERIAA
                     AAASGSPEEADLAQRILDANSQADAAEQDARARIEAANARADEAERAAQERIDRYTAD
                     ADQAEANAQERIATLSARAEEAQGSVTTPSTGDATGLIALAQRVHDEHVAEGEATRAR
                     LIDEGQSQHDELIATAQSQHDELVSSAQARHDELLATAQSQHDDLVSTAQARHDELLT
                     TGQSQHDTLIGDATTERDSILTGAREEAASLLAAANAERDQVIGDLDGQRSKIEELIA
                     SLRGHEQNYRLGLRTHIETALADLDAAPSVEEALAAPGAESLAAPEADGLDASEADGL
                     DTSEVDFEEESSKDAAAN"
     gene            32204..>32502
                     /locus_tag="PKU75_01695"
     CDS             32204..>32502
                     /locus_tag="PKU75_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79618.1"
                     /translation="MTPRQGTTTAAATSAPPRGGRTAATPPNGKAVPSGKKAAAKQST
                     ATTAAKAAATKAPASKAPAAKRAPATKAAAAKAAAGKEAAATKATGTKAAAAKAAA"
     assembly_gap    32503..32602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32603..33124
                     /locus_tag="PKU75_01700"
     CDS             <32603..33124
                     /locus_tag="PKU75_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPF79619.1"
                     /translation="ATKAATEKAPAKKAATNKAPAKRAAKAAPAAPPGGGSRHTGTGQ
                     RYAVKEGEQPWTAAEIAEIRATIEGDIATLRGEITEAEHEVAALMRQSGDGAGDDQAD
                     AGSKTLEREQEMSLANNAREMLLQDQHAIERIDDGTYGICENCGQPIGKLRLEANPRA
                     TLCVPCKSKQERR"
     gene            33097..33645
                     /gene="lspA"
                     /locus_tag="PKU75_01705"
     CDS             33097..33645
                     /gene="lspA"
                     /locus_tag="PKU75_01705"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPF79620.1"
                     /translation="MQEQTGTPLTHEDPTGEHPARPHTGRRRRAALVWVVALATLAAD
                     QATKLWALHTLTPGVSKPLIGDLIGLRLVRNAGAAFSSLDTATIVVTLVALLIVAGLL
                     YYVHTRALSRVSALCLGLIAGGALGNLIDRAAREPGFLRGHVVDFIDYFGWFVGNVAD
                     IAIVVAALVLVAVSWRSERSDG"
     gene            33638..34561
                     /locus_tag="PKU75_01710"
     CDS             33638..34561
                     /locus_tag="PKU75_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPF79621.1"
                     /translation="MAETKTVLVPEGLAGERVDAAVARLLGLSRSKAADLAGGGAILV
                     NSAAVAKSAKVVAGDLLEVTIPSPTGGPRVIPMDIPDLRIVHADDDLIVVDKPAGVAA
                     HPSVGWDGPDVLGGLLAAGYQITTSGAAERQGIVSRLDVGTSGLMVVAKSEYAYSRLK
                     QAFRTRTVDKTYHTLVQGLPDPLVGTIDAPIGRHPSYDYKFAVMDSGRASVTHYALIE
                     AFRAAALLEIHLETGRTHQIRVHFSALRHPCVGDLTYGADPVLAKRLGLERQWLHAVG
                     LGFEHPGTGEYVSFESPYAPDLELALEKLAG"
     gene            complement(34636..40488)
                     /locus_tag="PKU75_01715"
     CDS             complement(34636..40488)
                     /locus_tag="PKU75_01715"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPF79622.1"
                     /translation="MNLVLLGHQAWLSIDAIARTLTRLLVTHRHLLEWTTAAAAARRS
                     REGGAAYARQMAGGLVPAAAVLGTGLWRGPAAAAVAAVPALAWILAPAVAAAASRPHR
                     ELQLEASPVERAQLRRIARRTWLFFDTFVGQADNHLPPDNFQEDPEPVVAHRTSPTNI
                     GLYLLCIVSARDLGWIGTADATERLERTLGTLERLDRHRGHIVNWLETTTLRALEPRY
                     ISTVDSGNLAACLLTLSSATRAWSDTLLRAAPEPDPAGLTDALDVLSTTLTAIEDPAR
                     EAIAAAAKHVYAALATPPPAGQTQPAWAPVEAACAALVAACERLPDEPVYAETRLWAG
                     QVAASAHSLGADTALDPSSREKLAGRLRAVAAASRQLFDEMDFGFLRDDRRGLLSVGF
                     HIPTGRLDESCYDLLASEARLASYVAVIKRDVRTRHWFLLGRPVAAVGGGAALQSWSG
                     SMFEYLMPPLLLRSPYQGLLAKTNRLVVRRQMEYTSDSGIPWGISEAAYNARDVAYTY
                     QYSPFGVPGLGIMRGLADNLVIAPYATALAAMIDPGAALTNFGRLDGVGALGRFGFYE
                     SVDYTPARLPEGAGHAVVRCHMAHHQAMSILAIHNVVQQGALRDYFHAEPLVRSGDLL
                     LQEQAPTDVPVTHHRRETGQARVLRALTAPEERVFTGPAAYEPAVHHMSNGSLSLTLT
                     PAGGGHLRWQGVALTRWHPDPTSEEAGSHVFLRDEAEGDYWSATPLPILAGAHSVEVR
                     MADDKVTFTRNHHRFTTRLTYHLSPEADAAVRHLTIHNRQRKERRLTLLTYAEPVLAP
                     ARDDDAHPAFAKLFVATEFLPERGAVIAHRRRRNPSEPEVWFGEMIRVEGQAAIGALS
                     VETDRATFLGRGRTIREAGQLHRGGAASGRTGHVIDPVLSLQQRVRIPGDGEAQVTIW
                     SFAASTREDLLALMDRHSAPTAVPRLAMLAWTQSQVELRHLGISTREAGVFQTLAGRI
                     TYPIAGLRPSPADLARDAAPQSALWPLGVSGDLPIVVLRIPDTSHVPVVQQLIRAFEY
                     WRLRRFAVDVVLLNLEPTSYAQDLHLALESLAGSIRARTGSADSTGRIFVVRGDQTDP
                     ATLNALLASAAVVLDAGVGDVEDQLPRAAAPALGPSRTRPATPVPVHETPRPDLVAWN
                     GYGGFAPDGREYVTVIDGDALPPAPWSNVVAGPQFGFLATAEGAGTTWYRNARDNVLT
                     PWRNDTVTRTPSEAVYVRDDETGRIATPTALPIPTGRHLCRHGFGRTTYVHNTAYVHL
                     ELTQFVPDGETLKVGRLRVTNPTDRARRYTITTYAEAVLGQHRHGSAGRLISSRDAQT
                     GALFVRNPWSLQYADQVVFADLAGAQESVTADRGEFLGRQGTLAAPAAVLSRQPLSGR
                     TGPGLDPCLALRTGVHVPAGGTVEVCIVLGAAADADAARAAIARQRRERPEQQLATVE
                     AAWSRMLDQVEVRTPDPTFDLMVNGWLLYQTLAARVLGRTGFYQTSGAFGFRDQLQDG
                     MAVVLVDPALAREHLLRAAGRQFPEGDVQHWWLPEGGAGIRTRITDDVVWLATAVTRY
                     VAVTGDVGILDVEVPWLDGPALEPGQHENFFTPTVTTRTDPLYVHCTRALEHALLRTG
                     SHGLPLMGSGDWNDGMNRVGVHGRGESVWLGWFLLHTLDGFLPLARGRRDFAFVARCR
                     QAAEEVRQALEREAWDGQWYRRGYFDDGTPLGSVTRPECQIDSIAQSWATLSGQGDPD
                     RAAEALREVDRRLVMHEEGIARLFTPPFDTSEPDPGYVRSYPPGIRENGGQYTHGASW
                     LIFAYAEQGWEDKAGELFDLINPVWHGSGPERIETYRVEPYVVAADVYSVQPHVGRGG
                     WTWYTGSSAWLYRAAVEAILGLQRRGDRLLIRPCLPPSWTSAQADIRVGAAQYAVQFS
                     APPGAPGVRRRVATTTMDGVPVPDPQVLPLRDDGRRHTVIVTLARDDQPS"
BASE COUNT         5901 a        14717 c        14342 g         5559 t
ORIGIN      
        1 gggctggccg ccctgctcga gctgcgcctc ggcgagagcg ggaagcaggc cctcggcttc
       61 gccgtccacg tgccgcacta tctcgcccag gccgacttcc acgacggcgc cctgagcgca
      121 ctcaacgcca tcgtcgacgc cacgggcctg aacctgccca atgacgccct cgtccaggcc
      181 gccgccgagg gccgcgcgca gatcgacgcc gaggtggccg gcaaccccga gatcgcgggc
      241 gcggtcaatg cgctcgagcg ccagtacgac tccttcatcg aggcggccga caagccgaat
      301 ctcctgacgc cccaggcgaa tccgatcccc tctgcggacg agctcgcggc cgacgtcgag
      361 gagttcctca agtccgtctc cgacgagccg gacgccggct agtcccggtc acccgtcttc
      421 gcgcccctca gcccttggcc tgcgaccagc gttcgacgat cgaggtgtcg gtcacgaagc
      481 gcaccggcgc gccgccactc cagcggcgcg ccgcatcggt gagggcctgt tccacgcgtg
      541 ccgcgcactc cgcggcctgt tccgcgggca cgtgcacgag caactcgtcg tgcagacaca
      601 ggacgacctg ggcccccagg tcggccgtcg tcacccggac cgtcgacgcc caggccttga
      661 acagttccgc cgcggcgccc tggatgaggg cgttgcgggc ataacgcccg cgtgagcccg
      721 ggtcgagcga gcccgcggtg cggatgcgcc gcccgccgta ggtgcgcaga tcccgcgcgg
      781 ccacccccgc ctcgtatgcc cggtcgagga agcccatcgc caccggatag gcccgctgca
      841 gcccggccag cgcctccccg gcggcgccgg accgctgccc atacatcgcg gcgagcacgg
      901 ccaccttcgc ccgcgcccgc tccaccccca ggcgctccgc caccggcgcg tagagatcgt
      961 cggatgccgt tgcggcagca aaggattcgt cctgtgccac gcaggcgagc acccgcggct
     1021 cgatctgccc gagatcggcg cgcacgaaca gatgacccgg cgccgcccgc accgccggac
     1081 gcagcaccgc cggcagattg tgcaggccgt tctccgcggt catccgtccc gctgccccgt
     1141 cgcacgcggt ccaaccaccc cgcagccggt cgtccggccc gacatgcgtg tcgagccagt
     1201 gatagccgta tgtcgtcgcg atgcgttctt gggcccgcca ctccagcagc gccgggacga
     1261 gcgggtgcgc ctcgcggtag ggctccagca gccacttgcg cgtcgagggc acgtcgatcc
     1321 cgatcgcggc cagcagcgcc ttcaccgcgc cggggttgcg caggtcgaca ccgtcgtggc
     1381 cgggtgtgtg cgccagcacc cgggcgtcgc ggtccgcgcg gatgcgcagc ccgtcagccg
     1441 catcccgcgg ccgcgagccc gccgaccggg cgatgagcgc ctccatgccg ggccggtcga
     1501 tcggcaggcc gtcgcgctcc agttcgagac agagcacggc cgccgacgac tcggacaccg
     1561 ccgtcgcacg ccatacctcg ttgcgcccca aggcatccca gcgtcgcttg ccgcagtcgt
     1621 atgccgcggc cgcccacgcg tgcaggcgct gcggtgtggt ctgccaggtg ccggcgaccg
     1681 cgtccggccg caggtagccc gccggcgtcg acagcgcctc cggcggcacg ggctccgcga
     1741 gcgcgctgaa caggtcgtcg ggagccaggt ccggtatgcc gtcgccggcc agcccgtgca
     1801 ggcgcgccca cacccgcccc gggtcggccc gccaccggcc gcgctcgagc cggtgacact
     1861 cggcaaggtc gaggacccgc ggcggcggcg tcccggcacg cacgagtgcg cccaggtcgc
     1921 gcgccgccga ccagacgacg acccgtgggg catccgcgag cgccgcggcg acctccgcca
     1981 cctgctccgg gccgaacgct cgcggctgcc tgccgtcggt cacggcgaac ccggccggcc
     2041 cggcaacgag cgcgaccagg gacttcgggc tgggcacgag tgtgacccta tgccgcaggg
     2101 ccgacagcag ctgccgtccg ggaccaagct ggcgggcgcc aagcgccgtg gacgtgccga
     2161 cccatggcaa gatcgaccag ttcccgcata cccgaccacg cttgcgagac cacgcgtacg
     2221 agaccaaagg tgaccccatg ggactgttcg gcttcggcaa ggacgacgac gtcaaggaga
     2281 tggaaaagga catctccgtg accggctccg ccctggagcg tgccaagaag ggcgacgagg
     2341 gcgggatggc cggcgccgcg acgcggctga tggagcggct cctcgacacc ggcatcgacg
     2401 gccgcggtcc cctcgactcc gcccacaaga tcgccacgga cgcgctggcg aagcgcagga
     2461 ccaccgacga ggccgtcgac gagatcgtca aggaccacac gatgatggcc gccaccggcg
     2521 gcttcctgac cagcctcggc gggttcatca ccatgccggt ggcgatgccg gcaaatatcg
     2581 tcgagttcta tctcctcgcc acgcgcatgg tcgcgagcat cgccaagacg cgtggctacg
     2641 acatcgacaa gccggagatc cgctccgcga tcctgctgac cctcgtcggc gccgacgcgg
     2701 acgacctgct caagaaggcc ggcgtcgtgg ccaccggccg gctgtcgaac ctcgccgcgc
     2761 agcgccttcc ggggccgatc ctgatggtgg tcaacaaggc ggtggcgttc cggctgctcg
     2821 cgagcgccgg ccgcaacacc ctcgcccgct tcggcaaggg cctgcccgtc gcgggcggct
     2881 tcgtcggcgc cggtctggac acctacctga tccgcaagat cgccgactcg gcccgccggg
     2941 agttcccgcc ggtgacggat cgcaccgttc gctgacgcgg gaatcgcggc gcggcatacc
     3001 gcgttgactg aggcgatccg gtcacgccgt gtggccgcct cgaagaagga cccgccgtgg
     3061 ccgctcgttc ccgtctgctt ctggctcgct cgctgatgtc cgccgtgcgc tcggcgaccc
     3121 gccccggcgc cccctcgatg gccgagcgcg ccggttccgt gccgcgcctg gtgcgcgccg
     3181 tggccgccgg cgactaccgc ggtatgacgg tcggtcgcct cggcatgctc gcggtcgccg
     3241 ccgcatacgt catctcgccg gtcgatctgc tgccggaggg tttcctcgcg gtgttcgggc
     3301 tcgccgacga cgcgatggtt ctgagctggc tcgccgccaa cctggtcacg cagaccgagg
     3361 agttcctcga gtgggagcgc gccggcacgc cgggctcgaa cgccacggcg gccgacccgc
     3421 aggatgccgc caccagcgcg tatgccgggg ccggtgccac cgtctcgggc cgcgtcgtgc
     3481 gctgacgcgc cagcccgcaa tccacaggcg gagcggcaac ttcgcgcttt ccacaggggt
     3541 tttcgcgggt ctggcggccc gcgctcgcga cccgcgagcc tcgtcctatg acagtccata
     3601 tgaccagccc gggcgagttc ttgacctcgc tgccctatca gatcggcttc tatccgcacc
     3661 gcagcctcgt ggtggtgatg acgcgcgaca agacggtgat cctcgtcgcc cgcatggacg
     3721 tgccggagca tcccgtccat gccgatcgcg ccctcgacgc ggggtgcgcc gacctgcaac
     3781 gacagatcgg cgccggcaac ctcgacggcg cccatctcgc gctattcgac gactcgctgc
     3841 tgggcctggc cgccctcgtc cgggtcggtg accgactggc cgagagcggg gtgacgctcg
     3901 ggctggcagc tctcgtgagc gacgggatgt tccggctgct cggggccccc gacggaaact
     3961 gggacaccct gccgcggccg gaggactgtg caatcgtcgc cgagctggtg gcccaggggc
     4021 ggtcggtggc gcccgaccgg gacgcggcca tcgcgtgcct cgcgccctgt cgtccgccca
     4081 ccacggccgg caccgaactc accgcgcggg agtgcagcgt gcccgtgcac gcgatcgggg
     4141 agtggcgtgc cttgctcgcc ctggttccgc cggacgagcc ggacccgtgg cagcccagcc
     4201 ccgcgacggc gatccggctc tcgcgctcct tgctcgaccg cgacttccgc gatgccatgg
     4261 tggccgggct ggtgcccggc acgctgcccg actccgaact cgtcgagccg gctgccaccc
     4321 gcgccgcccg gatcctcgcc ccgctgcacg acgagttgga ccggcaccgg acggcacgcc
     4381 ggcacgtgga aggcggcgag cagccgccct ggcacggacc ggacctgcag cgggcccggt
     4441 tgcgcgaggt cctcgagcgc atcctcaccg tcgcgcgcct ggcccccgcc cccatcgccg
     4501 ccgggccgta tgccgtcgcc gccatcctgg ccggccggct cggcgaggga ttggtgtgca
     4561 ccggcgccat cgagcacgcg ttggaggccg accccgatca cgtgctcgcc aacatcatgg
     4621 ccgacatggt tgccgcaggt cacgtgcccg gccggccacc cggcacggtg gttcaggaca
     4681 gcggagcggc gtgatctcgg cgggcacgcg gcgagcgtca ggggaccacc ggcgcctcct
     4741 gcggcacccc tgccccgccc gtacctcgcc ttcggcggtg atcgggtgtg aactgcggcg
     4801 cgagtcgcgc gtgagggtgc gcgatctcgg gtagttttgg gggcaacggc cgcgaactca
     4861 aggaggagcc gcagtggcga tcgtcgttgg atatgtcccc accaaggaag ggcgggccgc
     4921 gctgacgcgc gccgccgagg aatgcaagtt gcgagggacc tcgctcgtcg tcgtgaactc
     4981 cgctcgcggt ggccgcgaca tggtcgatcc agagcagcgc gacggactgg acaacctggc
     5041 ctccgacctc aaggcggacg gcgtcgaggt cgaagtgcgc tcgcccgtgc gcggcctcga
     5101 gccggcggag gacctcatcg cgatcgcgga ggagatcaac gccgacttca tcgtcatcgg
     5161 cctgcggcgc cgcacgcccg tcggcaagct gatcctcggc tccaacgccc agcggatcct
     5221 gctcgattca ccctgcccgg tcctggccgt gaaggcggac tgaccagccg acgcgggcag
     5281 gcccggcacc cggccggacg gcgtggcagc gcgagccacc ctggcacggc acgaaatgca
     5341 gggtgggaat aacgctgtgc ggcctggcgc tttataatgt cgatgtcgat gtcgacgtgc
     5401 gcacctcggg ccaactgcgc aaggcataac aggcccgctg tccgacacgg tccacgcgac
     5461 gcctgtcacc agacaaccac ccccttaaga gtcgcttcgt cacgccctga cgaggcgact
     5521 cgatctcccc cgtgaaaggt cccgtgccgc tcgtgccccg ttccgcagcg ccagctcccg
     5581 agttgccccg tgagttcagc catacggcgc tgcaggacct ggtccggctc ggacatacag
     5641 cgggcgcgat ctcggcgaac cagctcgtcg cggccatgac cgcggccagc gtcaccgaac
     5701 gccggcgcaa agccgtcgtc cgggcgatcg aagaacaagg aatcgaggtc gtactcgaca
     5761 tgcccaccac cgccaagacc accaaggcag ccaaggcacc agcgaagtcc gcggcgaaga
     5821 gcgccccgac caagcgcgcg gccaagaacg aacccgcgga cgaggcggtc gagacgacct
     5881 cggatgcgac gagtgcggcc aagccggccc gcaagaccgc tgccaaggcc gccgccaagg
     5941 ctccggcgaa gactgccgcg aaggccgccg ccaagacgac cacagccaag acgaccgcga
     6001 agaaggccag cgcgaagacg gccactgaga cgacggccac tgagaagacg gccactgaga
     6061 agacggcccc gggcgacgcc gccgacgtcg ctgagcagcc tgaaggcgag gaggccctgg
     6121 ccgacgacgc cgagcccgag gacctcgagg atgccgaaga acccgacgtc gaggccgacg
     6181 ccgaggccga ggccgaggcg ccggagtccg cggacaccca ggacggcgag gaggcggacg
     6241 gcgaggccgc tcccgccgcg gccggcgagg acgaccagga cgagagcgcc ggcttcgtca
     6301 tccgggacga cgacgaggac gatgcgcccg cccagcaggt cgtcaccgcc ggggcgaccg
     6361 cagatccggt caaggactat ctgaagcaga tcggcaaggt cgccctcctc aacgccgagc
     6421 aggaggtcga actggccaag cgcatcgagg ccggcctgtt cgccgaggag cgcctgaact
     6481 ccggcgagat cgccgaccag aaactcaagc gcgaactgtg gtggatcgcc caggacggca
     6541 agagggccaa gaaccacctg ctcgaggcga acctgcgact ggtggtctcg ctcgccaagc
     6601 gctacaccgg ccgcggcatg ctcttcctcg acctgatcca ggagggcaac ctcggactga
     6661 tccgggcggt cgagaagttc gactacacca agggttacaa gttctcgacc tacgcgacct
     6721 ggtggatccg gcaggccatc acgcgcgcca tggccgacca ggcgcgcact atccgcatcc
     6781 cggtgcacat ggtcgaggtc atcaacaagc tggcccgggt ccagcggcag atgctgcagg
     6841 acctcggccg cgagcccacg ccggaggagc tggcccgcga gctcgacatg acgcccgaga
     6901 aggtcgtgga ggtgcagaag tacggccgcg agcccatctc cttgcacacc ccactcggtg
     6961 aggacggcga ctccgagttc ggtgacctca tcgaggactc cgaggcggtc gtcccggccg
     7021 atgccgtcag cttcaccctg ctgcaggagc agctgcattc cgtcctggac accttgtccg
     7081 agcgggaggc cggcgtcgtc gccatgcgtt tcggcctgac cgacggccag cccaagacgc
     7141 tggacgagat cggcaaggtc tacggcgtca cccgcgagcg gatccgccag atcgagtcca
     7201 agacgatgag caaactgcgt caccccagcc gcagtcaggt gctgcgcgac tacctggact
     7261 gaccccgagg cgctcgccgc tcgggcggcc ctgtttccgg tcccaccgcg aagggaacgc
     7321 ggctactcgt cgccgcggtg gcgcagctcg tcgccgaggc gggcgggcag gttcgtcacc
     7381 cgctgcacca gcgagcggac ggcggcccgg ccggcatacg gcagcaggcg ggcgcgcagg
     7441 cgcacgctgg ggctgcggct cgcgtcgacg ctctcgaaga ccgcatccac gtccggctgc
     7501 gggtcggcga cgggggtgcc tggggcggca taccgcgcca cctcgacggc gctgacgacg
     7561 cgccgcagcg ccgacgccgt gtcctggtcg agcaccgccg cctgggtgag atggctgtgg
     7621 acctgtcgcg gcgtgagcgt cggcggggtc ttgatgccca ggtcaccgat ccgggccacg
     7681 agatcctgcc atcgcgcctc gatgacgtca ctgtcgtcgt cggcggccgc gatccggcgc
     7741 cggcgggcga gcatcgaggt gaccggcagg acgagcatgc tgagcacccc cgctcccagg
     7801 gcgagcagga tccacgtgcg ggccgagatg gcggcgatcc gttgccgcag cgtgcgatcc
     7861 gccggcgtga catcctgcgt gcccgggtcg tcctggcccg ggttgaccgg ccgggtcgag
     7921 gaccgcgtgg cgctggcgga ggttgacgca ctccacgtcg tcgcggtgga ctcgggcagg
     7981 ttcgcccagc tcggcgccgc gccggagcgt gagcccgggg tcgggtcgaa gcggatccag
     8041 ccgaacggcg ggaagtacaa ctccggccag gcgtgcgcgt cgctggcccg cacctcccac
     8101 tcgccccggt cgagccggcc gggcaggtag ccgatcacca tgcgggccgg tatgccgcgt
     8161 tcgcgcgcca tcatgatcat cgccgtggcg aactgctggc agtagccgac ccgggtctgc
     8221 aggaagtggg tgatcgggtc gacgtcgctg ccttcgggca acggggccag ctgggtgtcg
     8281 taggtgaaga cgggggagcg cagccagtcc tggatggcga ccgccgtctc gaaccggttc
     8341 gccccctccg ggacggcgtc ggcgagggcc tcggcgatcg ccggtcgggc ccgctcctcg
     8401 acgagcaggc tgtcgtcttc cacggtctcg gcccacgggt cgggaccgac ataggactcg
     8461 gattgctcca gcagctccgg ggtgggggcg atgtcgatgt attcgacgtc atacgactcg
     8521 gcccggctga gcacctgcac cgtgccttcg ccgaactcga tggcctcgac gccctcgggc
     8581 atggtgagcc gggttgcgcc atacgggatc gcgatctgcg gcttggcgag ccgggatccg
     8641 gtggcgctca gcgtcccggc gcgcttggct tcctcgggca ggtcgtccag atagcgcacc
     8701 cacgtcgagg agatcgcctc ctgctgctgc agtggctcac ccttgctcac gttgcgtgcc
     8761 ggatactgcg gccgctgccg gaactgaccg tcgggccgat agtcctcgag gaccgccacc
     8821 cgcagcggcg acggcgagcc gacactcgtg cgataccgca gcagcggcgc gctcgacggg
     8881 ttctccaggc tggcccgcag gtcgagtgtc gtgctcaggg tgacggtgcc gtcggcgcgg
     8941 tcgatcgtgc cccgccccag cccgtcggcg agatagcggg tcgggaagtg cgggacgacg
     9001 gccgccagca cgatcgcgcc gatcgcgacg accgagccga gcacgccggc ggtacgggcg
     9061 aaggagcgca cggggtcggc cggtcgggcc tgggtatgcg gggtgcgcgg cagcaccgag
     9121 gcccaccgcc gcaggccgac ggtgcccgcg cgggccagca tcagcagcca cagggcggcc
     9181 ggcagcagga agtggatcca ccgcagcgtc gaactcgagt tcgccgcgcc gacgagatag
     9241 gcactgagga gggccaggcc ggcggcgccc ggtgaccggc ggctcacggc aagcgcgtcg
     9301 acggccagca ccacgacgag cgtgagcaac gagagcgtga ggacgatccc gggcgtggtg
     9361 ggggcggggg cggcatacgc ctgaatcgtc tcgcgcgcct cgacgaggat ctcgttgagc
     9421 gccaacaccg tgccccagtg gggcaggccg taccacgtct cgtcgcgggc atacatcagc
     9481 acgatggact cgacaccgac cagcagttgg accgctgcga cggccagcca gttgcgcagc
     9541 acggtccgcg ccagcacccc ggtgaccacg acgatcgcgg tgatgagcag cgccgggttc
     9601 acccacgcca ggctgcgcag cagcgtctgg atcggcagca tggccgccca cgacgcggcc
     9661 gcggcgagca gcgcatcgct ccagcgggcc tggcctctca tcgccggcct ctcatctccg
     9721 gccgccctgc agcgcggggg aggccgccgc ggccgcccag gcgtcgacga gcgaggtgcc
     9781 aggggtgatg accaccgcgc tccagccgcg ttcacgcagc ccggtcgcgg tggcggaggc
     9841 ggcgaccggg tcgtcgctgt cgctgaccgg aagcacgagc gccatcccgg gcgagccggg
     9901 tggacgcacc ctcgcgaggc gttcggtgtc gcgctcggcc atcgcggcga cgacggcgat
     9961 gcacaaggcg ccgctggccg cggcgtccgc tgcgagcgcc atcgtcgtga cgaactggac
    10021 gggttcgcgc aactcggcga cggccagttc ggtcaaggcc tcctcgaccc gcaggtgctc
    10081 gcccccatgg ccggagcgga tctcgccgcc caacgcgaga aaagtgttgg tgccgctgcc
    10141 gatgacctcg ttggcgatgg atgcggcggc cgtgaccgcc cactcgaagc tcgcggtgcc
    10201 ccggggggcc cgctcgacgg tggcccggtc gtcgagcagg agcacgatgc ggcgggtcgc
    10261 ggggcggtcc tcctggcgca ccatgagatc accggtgcgg gccgtcgccg gccagtggat
    10321 gcggcgcagg tcgtcaccct cgcggtgctc gcggacggac acatcgtcct cgccgtgcag
    10381 ggcgatcatg tgcgggatcg tgccctcgtt gccgaggccg ccggcgaccg tggtgccgga
    10441 cagggagacg atccgcggca gcacgatcaa gtcgccggtg ccccggacca ccgccagccg
    10501 cagcgtcatg tcgaaggggt ccttgacccg aaggccgagc ggtccgaggc gatggcgccc
    10561 ccgcacggtg gaccggacgc gatagttgat caggtgctcc gcgccggggg ccatccgggg
    10621 cacgacgaac cgggggcggt cccccagggc gtagtcgagt tgctcctccg ccatcaggat
    10681 cggggtcgtc gcggggccgg ggttctcgac gcgcaggttc acatcggacc actggtcgat
    10741 gggcacgatc gagggggtga cggtgcgggt ggtgcgcatc gagagccgtt ggcgccgggc
    10801 gaggagcagc atgagcagcg gcaggccggc gacgaggaag ccgatgcggg tgatatcgcg
    10861 gaaccccagc agcagaccgc acaccatgag cgcgactccc gcggcgagga agcagcggcc
    10921 gcgcagcgtg agggcgtcgg tcaggcggcg gcgacgtcgc cgaggcgccg tgctgccccg
    10981 cacgataccg gagcgaccac cgagtgcgga cacgccgaga gctttcagaa ccccggagcc
    11041 ggcacgcgga cccgctcggt cagcccgcgc aacacgtctg ccgggctgcg gcgggcgtgg
    11101 atcgcctcgc cggtgaggat gaggcggtgc ccccagaccg gcacgacgag gtcctggacg
    11161 tcgtcgggca gcacgtagtc gcgtcccgcc agcgcggcgt gcgcgcgggt cgcccgcagc
    11221 aggtgcagtg tcgcgcgcgg ggaggcgccg agccggacgg aggtgttgtc gcgggtggcc
    11281 gcggccaggt cgacgatgta ctggcggatg gcgtcggagg tatggacagt gcgcaccgcc
    11341 ttgatcagtc cgctcaccgt cgcggcgtcg gtcacgggcc gcagctcttc gagggggtcg
    11401 cgctcgccgt gggattcgag catgtccatc tcgctgcgcg atgacgggta gccgagggag
    11461 acccgcgcca tgaaccggtc ccgctgcgcc tcgggcagcg ggtaggtgcc ctccatctcg
    11521 atggggttct gggtcgccat gacgatgaac gggtggggca gggtgtaggt gcgcccgtcg
    11581 acggtgacct gcccctcctc catgcactcc agcagcgccg actgggtctt cggggaggcg
    11641 cggttgatct cgtcgccgac gacgacattg gcgaagatcg caccaggccg gaactcgaag
    11701 gcccgcatgt cctggttgaa catgttgacg ccggtgacat cgctgggcag caggtcgggc
    11761 gtgaactgga tgcgccggac ggtgcagtcg atggagcggg cgagggtctt ggcgagcatg
    11821 gtcttgccga cgccgggcac gtcctcgagc agcaggtgcc cctcggcgag cagcgccgtc
    11881 agggccgtct ggatgacctc gggcttgccc tcgatgacgg tcttcatggc gttggccatg
    11941 ctggtggtga tctcgaggac cgattcgagg gagcgcgccc ggtccggagc cgccgcctgc
    12001 tgccgcgtct gctgatcctc cacgatgctc cccaacgtcg ttcgtcatcc ggccttcgcg
    12061 gccggcccgg tctcgtgccg tcgtcgcatt ttgcctcagg ccgcgcgatt cggggctttt
    12121 gggaccggtt cgtgaccttc gcacctcggc gatctccact cgctggacca aacgggccaa
    12181 aatcgcccca ctttcctcca caacgtgtga cctgggaatt tgcctcgtac taggcgacaa
    12241 agtccgcaaa acgtcaccaa cttggtatgt aggtggggta gagtggggga aatcggaggc
    12301 acggggcgcg catgcggaga gggaaggcgg tggccggttc atggacgtcg agttcctcgg
    12361 tcaccacagc ccccggctcg acgagaaggg gcggttgttc ctgcccgcga agttccgcga
    12421 cgacctgggc gacaagatcg tcgtgaccaa gggtttcgag cgctgcctga ccatctatcc
    12481 gcaccccgtc ttcaaacagc ggatcctcga cccgctcaac gccgccaaca ccagcagcaa
    12541 gaccgtccgc tcggtgcgcc ggcagctgct cggcgacgcc tcgacggaga accccgaccg
    12601 ccagggccgc gtgacgatcc ccggcgtcct gcgcagctac gccggcctgg acaaggactg
    12661 cgtcgtcatc gggcagggcc actacctcga ggtctgggac agcgccacct acgaagccgc
    12721 caatgcaacc gacggcacca cgctcgagga cttgcacgac gagggggtcg tcctgctctg
    12781 acgttcggtg gtccccggcc tccagccgtc ctcccggccc gacttccccc ggatcggggg
    12841 cggcggatgg ggaccaggga ccaccgcccg cgctgctcag gccgaccacc ggcataccca
    12901 ccgcacgacc cgcaccgacc cgaagtgatg gcccgcatga acaggagcgc acccgccgac
    12961 caccggcacg tgcccgtcct gctcgaacgc gtcgtcgagg tgctggcccc cgccgtgcgc
    13021 gagccgggca gcgtcgtcgt cgacgcgacc ctgggcctcg gcggccacgc gcaggccctc
    13081 ctcgacgcct gccccgaggc gacgctcgtc ggcatcgacc gcgacgcgca ggcgcgcgac
    13141 gctgcggggg agcggctcgc ggcatacggc gatcgggtgc ggatcgtcga cgccgtctat
    13201 gacgaactgc ccgaggtgct ggccgggctc ggcatcggca aggtgctggg catcctgttc
    13261 gacctcggcg tctcctccct ccaactcgac gccgccgagc gcggcttcgc ctaccgcttc
    13321 gacgggccgc tggacatgcg gatggacccc agccgggggg cgagtgcggc gcaggtgctg
    13381 cgcgactacg acgccgccgg cctgacccgg gtgctgcgcg actacggcga ggagcggtat
    13441 gccgccaaga tcgcccgcgc gatcgtcgcc gagcgcgagc ggaccccctt cgagacctcc
    13501 ggccaactcg tcgagttgct cacccgggtt atccccgcgg cggcccaacg cagcggtggg
    13561 catccggcca agcgcacgtt ccaggcgctg cggatcgagg tcaacgacga gttacgggtg
    13621 tgggagcggg ccctgccggc cgccctggac gccctggccg tcgggggccg catcgtcgtg
    13681 ctgtcctacc actcgctcga ggaccgcatc accaagacca atctggccgc cggtgcccgc
    13741 agcagcgccc cggtcgggct gccggtcgaa ctgcccgaac accgccccta cctgaagctg
    13801 ctcacccgcg gggccgaaga agccggcgcc gccgagcagg ccaccaactc ccggtccgcc
    13861 tcggtgcgga tgcgggccgc cgaacgcatc cgggacacct cgaagggatc acgatgagct
    13921 ccaccgcact gaaactgagc ggcgccccgg ccaaggcccc cgggcgccca ccgctgtcgg
    13981 tcgtgcgcgg gggcgctggg gtgcgccagg cgtggttctc cctggcgatg ctcgccgtgc
    14041 tcgtcctcgg gctgctcgcg accctcgtca tcaacaccgc cctcgccgag ggcagctacg
    14101 agcgcgggaa gctcgtgacc gagtcgaccg tcctcgccga ccggcaggag tcactcacca
    14161 ccgacctgga caacctgcgc gcaccggcgg ccctggccaa ggaggcaatg tccatgggga
    14221 tggtcccggc ccagtccgcc gccttcgtcc ggctcgccga tggggccatc ctcggcgtgg
    14281 cggaggcagc aacggccgac aagcgcttta cggtgattac ctcaccgaca ctgcccaaat
    14341 cgaccgcgaa atcctccgcg gcggccccga gttcggcccc cacctcgaag gactgagacc
    14401 cagtgactcc ccgtagctcg ccaccgcagg tgcggcagcc ggcgaggagt cgcggcggtg
    14461 ctgcggcacc gccgcgcccg cgcgggtccc gcccggcagg agctggccgc ccggcagcgg
    14521 atcgcccggt ggcggatcgg cccgtggcgg atcggactga ggggccccga gcgccccgcc
    14581 ccgccgcccg cgcgccccgc cccgatcgcg ccccccgccc tgatcgcacg acccgccccc
    14641 agcggaccgc gcgcggcgcg cggcccctcg ctcggcccgg cgccaacccg cgggtgcgcg
    14701 cccgcggcat actcatcggc ctgctcgtcg tcctgagcct gttcgccgcc cagctggtgc
    14761 gcgtgcaggg cctcgacgcg ggcgcggcgg cggcgctggc catgcagacc cgcgtctaca
    14821 ccgagaccac gccggccctg cgcggcacga tctacgacgc gaccggcacg atcctggcct
    14881 cgagcatcga aaagcgcatc gtcatcgccg accccaaggc ggtcgtcgaa tacaagaaga
    14941 aggtcgacgg caaaaccacc aaggtcggcg ccgccggggc ggcggccgac ctggcaccgc
    15001 tgctgaagat gtccgaggcc gatgtcctga aggagatcac ccgtcccaag acccgctacg
    15061 tcgaggtcaa caagaacgtc tccccgctca cctggcacac gatcgccgaa ctcggcatcc
    15121 ccggcatcta cagcaaacgc acttccgtgc gcacctatcc ccagggctcg accgcggcca
    15181 gcctcgtcgg cttcaccggc gagaaccagg agggcgccgg cggcatcgag acctatctca
    15241 actccagcct gcgcggggtg ccgggcgagc tgaagtacga agtcggccag gacggcatac
    15301 ggctgcccat gggcgcctcg accgaatccc cggccaagac cggccgctcg gtgcagctga
    15361 ccatccgcaa cgacctgcag tggtacgccc agaacgtgct ggcccagaag atgatcgacg
    15421 tgcgggcatc ctccggcacc gtcgtcgtga cgaacgcgaa gaccggcgac ctgctggcca
    15481 tcgcgtccta cccgaccttc gaccccaaca cgaagatcgc cgacaacgcc cagttgacca
    15541 acctggcgtt caccgaggtc ttcgagcccg gttcgacggc caaggtcatc accgcggcgg
    15601 ccgcgctcga ggagggcaag gtcaccccct cgacgccgat gatcgtcccc tactcgctgc
    15661 gccgctccga cggacagatc agcgacagcc acagccaccc gaccgagtac ctcaccttcg
    15721 ccggcgccct ggcccagtcg agcaacaccg gcatcgtcct cgccggcgag cagatcccgc
    15781 cggcgacgat ggagagctac ttccgcaagt tcggcttcgg gtccaagtcg gccgtcaact
    15841 tccccggcga gagcgccgga ctgctcgcac cggccggcca gtggagcggg acccagcgct
    15901 acacggtcat gtacgggcag ggcttgtcgg tcacggcgct gcaggcggcc ggggtctatc
    15961 agacgatcgc caacgggggt gtgcacatcg ccccgaagct cgtcacggcg atggcggacg
    16021 cgaacggcaa gctgcagccg acaccggagt ccgccaagac ccgggtgatc tcgcaggatt
    16081 ccgcccgcca gctgtcccag atgctcgagg gcgtcgtcgg cgccgacggc accgcgccgg
    16141 ccgcgcagat ccccggctat cgcgtcgccg gcaagaccgg cacggcgttg cgttatgaca
    16201 gcagggtcaa ggggtatgcc gggtacaccg cgagcttcat cggctacgcc ccggccggcg
    16261 atcccgaact cgtcgtctcg gtgatcgtcc agaaccccaa gaaggagtat tacggtggct
    16321 ccgtggctgc accggtcttt cacgacgtca tgctctatgc gctgacgtcg ctggacattc
    16381 ccccgacgcc gggtgacacc gagccaccca agctgaccct gaaactggac gagacgcccg
    16441 acccggacga cccggccgtg ctgcgcgacc gacggtgacc cggacccgat gatcgacatc
    16501 cgtccagagc gtcgtgctcc ggtgccgctg gcacgcgtgg ccgccgtggc cggcgccacc
    16561 ggacccactg cggacgccgc ggccgtcacg gtcaccgggg tcggcctgga ctcgcgcacc
    16621 atccgccccg gggacctgta tgccgcgctg cccggcgcca atgtccacgg cgggcggttc
    16681 gtggacgggg cggtggagcg gggtgcggtg gccgtgctca ccgacagtgc gggcgccgct
    16741 ctcgccgctg gcacgccggt cccggtcctg atcgtcgccg acccgcgggc cgccctcggc
    16801 caggtggccg ccgagatcta cggcacccgc ccgccggggc tggacctgat cggggtgacc
    16861 ggcaccaacg gcaagacgac cacggcctat ctgatcgact cggccctgcg tgccctcggg
    16921 cggcgcaccg ggctgatcgg caccgtcgag acccggatcg gggatcagcg ggtcaagagc
    16981 gttcgcacca cacccgaggc gaccgacctc cacgccctcc tcgccgcgat gcgcgaggcc
    17041 gacgtggcca cgtgcgtgat ggaggtgtcc agccacgccc tcgcgatgca ccgcgtcgac
    17101 ggggtcgtct acgacgtcgc gctgttcacc aacctctccc aggaccacct cgactatcac
    17161 ggcacgatgg aggactactt cctggccaag gccgaactgt tcacgcccga gcgggcgacg
    17221 cggggcgtgg tgtgcgtcga cgacgcgtgg ggggcgcggc tggccggcat ggcgaccatt
    17281 cccgtcagca cggtgagcag caccggcgcc cccgccgact ggcggctcga cgcggcaccc
    17341 gacggcgccg agttcacctt gaccggtccg catacgcagc tcgccctgcg ctccgccctc
    17401 cccggagact tcaaccgggt caacacggcc ctggccacgc tggcgctgct cgctctcggc
    17461 cacgacgcgg gcgccgtgcg ggaggccgtg ctgaccgacc cgcatgtgcc cgggcggatg
    17521 gagaaggtcg cgcccgcagc cggcagcgat gccccgctcg ccgtcgtcga ctacgcccac
    17581 acccccgaag ccgtcgccgc ggcgctggcc gcgctgcgcc cgcaggcggt gccgggcggg
    17641 ctcgtcgtcg tcctcggcgc cggcggcgac cgggaccgcg gcaagcgcgc cgccatgggt
    17701 gccgccgcgg cacgcgaggc cgatatcgtc atcgtcaccg acgacaaccc gcgctcggag
    17761 gacccggcgg tgatccgggc ggcggtggtt gccggggccc gagaaagcgc gccgcacaag
    17821 gacattcgcg agatcgccga tcgcgccgag gccatcgccg ccgcagttgc ggctgcggtc
    17881 gcggcgggtc gtggcgccgt cgtcgcggtc gtcggcaagg gacacgagac gggtcaggag
    17941 atcggcggcg tggtgcatcc cttcgacgat cgcgagcagg tgcgagcagc cctcgacgcg
    18001 gcatacggtc aggcctcgtg atcccgacga ccctgaccga gctggcggag gtgaccggag
    18061 gccggatcgc cggactcggc gagagcgagg ccggcgggct gacgatcacc ggcgccgtcg
    18121 tcaccgactc ccgcgaagcc gagcctggca gcctgtatgt cgcccgcatc ggcgagcacg
    18181 ccgacgggca cggcttcatc gccggcgcgg cacagcgagg cgcggtcgcg gcactcgtca
    18241 cgcaggacac cggaccattg ccccgggtgg tggtcgccga cgagcaggcc gcattcgtcg
    18301 cgctcgcccg ccacctcgtc gacgcccggc cgcagctgaa cgtcgtcggc atcaccggct
    18361 cctccggcaa gacctcgacc aaggacctgc tcggccaggt gttggcccgc gacggcgaga
    18421 cggtggcgcc ggtcgggtcc tacaactccg aggtcggtgt gccgctgacc gtatgccgca
    18481 tcaccgactc cacccgccac ctcgtcgtcg agatgggcgc ccgcggcatc ggccacgtgc
    18541 gctatctgac cgagatcgcg ccgccgcgca tcggcatcgt cttgaacgtc ggctcggccc
    18601 acgtggggga gttcggatcc caggcggcca tcgccgtggc gaaatccgag ctggtgcagg
    18661 cgcttccgag cgacggcgtg gccatcctca acgccgacga cccgctcgtc cgggccatgg
    18721 ccgggcgcac cacggcgcgg gtcctgctcg tcggtgaggc gcaggacgcc gaggtgcggg
    18781 ccaccggcgt gacgctcggc gccgacggcc gggcgcggtt ccgctgctcg gggccgttcg
    18841 ggaccgccga ggtgcacctg gccgtcgccg gccggcatca ggtcggcaat gcgctggccg
    18901 tcatcgccgc cgcgcacgag tccggcatgg ccatcgagac gatcggcgcc gccctgcgtg
    18961 atgccgggcc ggtcagccgc taccggatgg aggtcgccac tcgggccgat ggggtcacca
    19021 tcgtcaacga cgcctacaac gccaatcccg agtcgatgac ggccgccctc gacgcggtgg
    19081 cggcgatggc gggcggtcgc cgggccgtgg ccgtgctggg gcccatgctc gagctgggcg
    19141 ctgaggccga ggcggcccac cgggcggtgg ggagcgccgc ggccgtgcat ggcgtccata
    19201 cggtcatcgt cgtcggcgac caggcccgcg gcatcggcga gggggcgagc agcggcggct
    19261 cgcacgtcga atatgcgccg gatgtggagt cggcttacgc tatgaccgtc gacatcgtcg
    19321 aagccggaga catcgtgctg gtcaagtcca gcaatggcgc cggcctgcgt ttccttgggg
    19381 accggctgcg cgacacgccc gtgtccgcta aggaggacct cacgtgctag ccgtactcgt
    19441 ctctgcggtg gtggcgctcg tcatcggcct gctcgggaca ccggcgtaca tcaagttcct
    19501 cgtcaagcgc ggctacggcc agttcatccg cgacgacggc ccgacctccc accacaccaa
    19561 gcgcggcacc ccgaccatgg gtggcacggt catcatcgcc gcgaccgtgg tcggctactt
    19621 cgtcgcccac ttctcgacgg ccgtcatcgg ctcgaccggg tatgccgcgc cgaccgcctc
    19681 cggtgtcctc gtgctctttc tgatggccgg gctcggcctg gtcggcttcc tcgacgacta
    19741 catcaagatc agccggcagc gctcgctcgg cctgcggagc aagcagaaac tcgccggcca
    19801 ggcactcgtc gcgatcatct tcgccgtgct cgccctgcgc tttcccgacg gcaatggcac
    19861 ccatcccgcc tcgacggcca tctccttcgt gcgcgacacc aaactcgacc tcgccttcgg
    19921 cggcaccctg gtcggcatcg ccctgttcgt catctgggcc aatgtgatga tctcgggcac
    19981 gtccaacggc gtgaacctca ccgacggcct cgacgggctg gccaccggcg cctcgacgat
    20041 ggtgttcgcg gcctacgtcc tgatcggggt ctggcagtcg aaccagaact gcgccacctc
    20101 gctgaacatc aacaactgct acgcggtgcg cgacccgctc gacctggccg tcgtcgccgc
    20161 ggggggcatg ggggcctgct tcggcttcct gtggtggaac gccgccccgg cgaagatctt
    20221 catgggcgac accggatcgc tcgccctcgg cggcgcgctg gccggcctgg ccatcaccag
    20281 ccgcaccgaa ctgctcgtca tcatcctcgg cgggcttttc gtcacgatca ccctctcggt
    20341 gatcatccag gtcgcctcct tcaagtcgac cggcaagcgg gtcttccgca tggcgccgct
    20401 gcagcaccac ttcgaactga aaggctggca ggaggtcacg atcgtcatcc ggttctggat
    20461 catcgccggc ctgttcgtcg ccttcggtct gggcatcttc tatgcggagt gggtcgtcgg
    20521 tgccctctga ccgactccag ggcctgaccc acggcggcgc cgactggtcg ggcctgcgcg
    20581 tcctcgtcgc cggcctcggc atcagcggct tcgccgccgc cgacgccctc gccgaacgcg
    20641 gcgcccgggt cgtcctcgtc gacggccaga cgcccgcagc aggcagcgaa ctcgctcagc
    20701 gagccacgat cctgcaggtc ctcgacgtcg aggtgcgcac cgggccgcag gcgaccgccg
    20761 ccctccccgc ggacctcgac ctcgacgtga tcgttacctc accgggctgg cgccccgacc
    20821 agcctcttct cgccgctgcc gcggcagcgg gcatccccgt ctggggggag gtggagctgg
    20881 cctggcggat gcggccgccc gagggcgcgg cgccctggct gacgctgacc gggaccaatg
    20941 gcaagacgac aacggtgcag atgctcgcga gcatcctgcg cgccgccggg ctgcgcgcga
    21001 ccagcgcggg caatgtcggc accccgatcc tcgaggccgt gctccacccc gagccgtatg
    21061 acgtcatcgc cgtcgaactc tccagcttcc agttgcactg gtcggagtcg atcagcccgc
    21121 tggcctcggc gtgcctgaac atcgcgcccg accacatcga ctggcacggc tcgttcgagg
    21181 agtactggcg cgccaaggcc aaggtctacg cccgcaccga ggtggcgtgc atctacaacg
    21241 tcgccgacga cgagacccgg cgcatggtcg aggaggccga ggtggtcgag ggggcgcgcg
    21301 cgatcggctt caccctcggc ataccgtcgg tctccatggt cgggctcgtc gaggacgtgc
    21361 tggccgaccg ggccttcgtc gccgaacggg ccacgagcgc ggccgagatg ggcaccctgg
    21421 ccgatctgtc ccagggtgat gtcccgccgc cgccccacgt cgtcgccaat gccctggccg
    21481 ccgccgccct ggcccgggcc tatggcgtgc cgaccggagc cgtccgcgac gggctgcggg
    21541 cctttgtccc cgaaccacac cgggtcgccg aggtcgccac cgtcggcggg gtgcgcttcg
    21601 tcgacgactc caaggccacc aacccgcacg cggccgcggc gtcgctggcc gccttcccct
    21661 ctgtcgtgtg gatcgccggc gggctgctca agggcgccga cgtcgacgaa ctggttgccg
    21721 cccacgccgc ccggctgcgc ggcgtcgtcc tcatcggtgc cgatcgcgcc cggatcggcg
    21781 aatccctcgc ccgacacgcg ccggatctcc ccgtcgtgga cctgtccgac actgacactg
    21841 gtgcgatgga tcgtgccgtg gcggccgccg ccgacctcgc ccagcccggc gatgtcgtgc
    21901 tgttggctcc cgcggccgcg tccatggaca tgttcgccaa ctacggcgcc cgcggcgacg
    21961 ccttcgccga tgcggtccgc cggctgccga ccaccgggga gtgatccgtg agcagcacca
    22021 ccgcgtcgcc gctgtccagc cgcgccgccg gccggcgacg gctggcgcgc ctgcgcgaac
    22081 tgcttgacag ccccgtcgcg acctactatc tgctgctgtc ggtcacgacc ttgctgacgg
    22141 tgatcgggct ggtcatggtg ctgtcggcgt cgatgatcgt ctccatcaag gacagcgaca
    22201 acccctacgc cgtcttctac acccaggcga tcttcgccgc gatcggcacg gtcgcgatgc
    22261 tcatcgcgat gcggctgccg ctgggcttct ggaaacgcat cggcggcgcc gccctgatcg
    22321 gcgcgatcgg gttgcaggcc ctcgtgttct cacctctcgg tttcgatgtc caaggcaacc
    22381 gcaactggat caagctgccc ggcttcagcc tgcagcccag cgagctggcc aagatcgcgc
    22441 tgatcctctt cggcgcccgg gtgctgaccc agaaacgtcg cttcctcggc tccttcaagg
    22501 agtcggtctt ccccttcgtg ttcccggccg cagcgatcac cctcggcttc gtcatgctcg
    22561 ggcacgacct cggcaccgcg atcgtgctcg cggcgatcgt cggagggatg ctgctggccg
    22621 gtggtgtgcc cttgcggtgg ttcgggctcg ccgggctcgg gggcctggcc ctggttgccg
    22681 cggcgacgat gaccagctcc aaccggatgc agcgcatcag catctggctc gaccccagcc
    22741 agtgtgtccc gagcaacgag cggctctact acgacatctg ccgccagccg ctgcacgccc
    22801 gctacgccct cgccgacggc ggcctgacgg gcatcggcct gggcgcgtcg aaggagaagt
    22861 ggcagtggct ctccgagccg cacaacgact tcatcttcgc gatcatcggc gaggaactcg
    22921 gcctgatcgg tgcgctcatc gtgctcgcgc tcttcgccct gctcgggtac gccgcatacc
    22981 gcctggtcgt gcgcaccgac gacttcttcg ttcgcatcgc cgcctccggt gtgatggtgt
    23041 ggatcctcgt gcaagccatc gtcaatatcg gcgccgtcat cggcgccctg ccgatcatcg
    23101 gcgtcccgct gcccttcgtc tcctcgggcg gctcctcgct catcacgacc atgctcgcca
    23161 tgggcatcct gctcgcgttc gcccgcgccg aacccggctg cgccgaatcg ctgtccgtgc
    23221 gccccaagac cgtgcgtgac tcgatggccg tgctcagccg cacccgcggg aagcagcgat
    23281 gaccgacgcc ggtccggccg ccaccacgcc ggcctcgtgg cccgctgccg tcctgctcgc
    23341 cggcggcgga tcggccggtc acgtctcccc gctcctcgcg ctcgccgaca aactccagca
    23401 gttgcgtccc gacatgggga tcgccgcgct gggcaccgag accggcctgg aggcgcggct
    23461 ggtgcccgcc cgcggctacc ccctgcacat ggcgcccaag gtgccgctgc cgcgccggcc
    23521 cgggcccgac ctgctgcggg tccccggcag gcttgcgggc gcgatcaagg ccgccgaggc
    23581 cgccatcgac gccacggggg cggaggtggt ggtcggcttc ggtggctacg tctccacgcc
    23641 ggcatacctt gccgcccgcc gccgcaagct gccgatcgtc atccacgaac agaacgcccg
    23701 cccgggcctg gccaatcgcc tcggcgcccg catgaccccc ttcgtcggca cgaccttcgc
    23761 cagcaccaag ctggcccacg cccggcgggt cgggatgccg ctgcgacccg agatctccac
    23821 gctcgaccgg cccgcccgcc gcgcggaggc gctgcagcgc ttcgggctgg cggaccaccg
    23881 ccccaccgtg ctggtgaccg gtggctcgct cggggccaaa cgtctcaacg aggccttcgc
    23941 cgcccgcgtc ctcgagctgt ccgaggccgg catccaggtg ctgcacgtca ccggcaacgg
    24001 caaggcgttc gccgcgcccg acaccggagc gggggcgcgc tatgtggtcc tcgagtatgc
    24061 cgacgcgatg gaactcgggt atgccgccgc cgacctcgtc gtcgcgcggt ccggagccaa
    24121 cacggtgtgc gagctgagtg ccgtcggcct gcccgcggtc tatgtgccgc tgccgatcgg
    24181 caacggggag caggaactca acgcggccga tgtcgtcgcg gccggcggcg gcctgctcgt
    24241 gcccgacgcc gaactcaccc cgacatgggt gaccggcacc ctcatcccgc tcgccaccga
    24301 cgccgggcgc ctgcagtcga tgtccgccgc cgctgcggcg gtgggggagc gcggcggcga
    24361 tcagctgctc gccgacctcg tcgcgcaggc ctacgccgcc gggcgagcgg gaggccactg
    24421 atggcccccg gccggctggg cgtcaacccg cggttcgact tcgccgcccc gacgaccccc
    24481 gcgagcgaat tgggcgccac ccatttcgtc gccatcggcg gcagcgggat gtccggcatc
    24541 gcccagctgc tgcacgacct gggggtcgag gtgagcgggt gcgacggggc cgcgtcgccg
    24601 gccgtggagc gcttgcgcgc cgccggtatg gcggtgtcca tcggtcacga cccggggcac
    24661 ctggacgggg tgcgaaccgt cgtggtctcc tcggcgatcc ccgagaccaa cgccgaactc
    24721 acggccgccc gggccggggg gctgcgggtg ctccatcgcg cgcaggcgct ggccagtgcc
    24781 atgggtgacg atgtccgggt cgcggtcgcc ggggccaacg gcaagaccac gacgtcggcg
    24841 atgctgacag cggtcctgcg ggcggcaggt gccgacccct ctttcgcgat cggctccgag
    24901 ctggccgacc tgggccgcaa ctccctccgc gggactggcg gcgtgttcgt cgccgaagcc
    24961 gacgaatccg acggatcctt cctcagctat cgaccgcacg tcgccgtcgt caccaatgtc
    25021 caacccgacc acctcgactt ctacggcgat ttcgcgcacg tccagcaggc gtatgccgcc
    25081 ttcgcccgct ccgtcgaacc cggtggctgg ctgatcgccg cggcggacga ccccggatcg
    25141 gctgccctgg cccggttgct gcggtcggag gggcggcagg tcctgaccta cggcgaggac
    25201 cccgccagtg acgtccggat cgaggaggtc gagctacacg gcctgacctc gaccgctcgc
    25261 gtcgtgtccg accgcggggc agtgccgctg cgggttgccg cgcccggtcg gcacaatgtg
    25321 cacaacgcgg ccgccgcgct cgccgccggc tggctggccc tgggccgcga ccccgcgcaa
    25381 ctcacggccg ggttggcagc cttcccgggc gtgcaccgcc gcttcgaacg caagggcgag
    25441 gtggacggca tcgtcgtcgt cgacgactac gcgcacaatg cgcccaaagt agccgcactg
    25501 gtcgccgccg cgcgggccgt ggtcggtgcc ggcggggcgg tccgcgtgat cttccaaccc
    25561 cacctgtact cccgcacccg cgactttgcc gccggtttcg ccgccgggct gcagcccgcc
    25621 gaccagatcg tgctgcttcc ggtctacggc gcccgagaga cccccatggc cgggatcgac
    25681 tcccacctga tcgtcgagcc gttgcgggac gcgggggccg acgccacgct ggcggccggc
    25741 ttcgacgagg cgatcgacct cgtggcgtcc cgggccgggc cgggcgacct gctgctgacg
    25801 gtcggtgcgg gcgatgtcac ccgcatcggg ccccgactgc tcgccgccct ggcggcccga
    25861 tcacacgcgg gaccggatcg tgcgtgagac ccctccgtcg acgctcgagt cggagcgcac
    25921 cgagcagcgt cgggtgctct ccttcgccga cgggtggaag cgccgggctc gagcactgcg
    25981 ccgccggcgc gcgggcaagg tggtgtggct gatcggtgct gcggtcctgg tgctggtcct
    26041 ggcctggctg gcgctctggt ccccgctgct cgcggtcacc gaggttcagg tctcggggac
    26101 cactccggcc gtccgctcac tcggcaaatc tgtcgggggg caggaggtgg ggcgctcgct
    26161 gttgcgcgtc gacacggcag gcgtcgaagg ccggctggag gaggactccc gggtcgcgga
    26221 tgccaccgtc cgccgcggat tcccgcggtc gctcgtcatc gaagtcgtcg cccgggtgcc
    26281 cgtgctcgga ctccagaagt ctcaaggtaa agtagagctt gtggatgtgt ccggcgtgac
    26341 catcgagacg gtgtcggagg cgccagccgg cgttccggtc gtgcaggggg ccaccggggt
    26401 ggcgagcggc gacggcgtgc atgcgaccgt gcaggtgatg gccgcccttc cggagtccct
    26461 gcgcactcgg gtgcgtgatc tgcacatcga cgactcgggc tcggtttcgt tcgtcatctc
    26521 cgcgacgcgc atcgtgtggg gcccggccga ggaatcggaa ctcaaggcgc gcctggtcca
    26581 gatcctgatg agcaagaagc ccaagacgat cgacgtcagc gcccccgaga cccccgtcac
    26641 gacctgacgc ggtcggcgcg gaccttcgac tcggacgcgg gacgggtgtg atggacgggg
    26701 ccggcgatac gcgtgaggag agcgcgcgca acttctttcc cgcgacacgc gcggacggcg
    26761 tttgcaccag tgccttccga cgcataccgt cgacccggaa ggcttgcagt gcaatctgaa
    26821 cctcgactag agggtgagga ttggcggtag gccgagccgc aggagaccgg gcctgcggtg
    26881 ccaaacggat atccgagaac gaaagctagg aaggccccac gaagtggctg ctccccagaa
    26941 ctatctggcc gtcatcaagg tcgtcggcat cggcggcggc ggtgtcaacg ccatcaaccg
    27001 gatgatcgaa gccgggttga agggcgtcga attcatcgcc atcaacaccg acgcgcaggc
    27061 gctgttgatg agcgacgccg acgtcaagct cgacgtgggt cgcgacctga cccgcgggct
    27121 gggcgcgggc gccgaccccg aggtcggccg gcgggcagcc gaggaccacg ccgaggagat
    27181 cgaagaggtc ctcaagggcg ccgacatggt cttcgtcacc gccggtgaag gcggcggcac
    27241 cggcaccggc ggcgcaccgg tcgtcgcgaa gatcgccaag gggctcggcg cactgaccat
    27301 cggtgtcgtc acccgcccct tcaccttcga gggccgccgc cgcgccaacc aggccgagac
    27361 cggcatcggc tcgctgcgcg aagaggccga caccctcatc gtcatcccca acgaccggct
    27421 gctgtcgatc agcgaccgca atgtcagcat gctcgacgcc ttccgcagcg ccgaccaggt
    27481 gctgctctcc ggtgtccagg gcatcaccga cctgatcacg acgccgggcc tgatcaacct
    27541 cgacttcgcc gacgtcaagt cggtcatgtc cggggccggt tcggccctca tgggcatcgg
    27601 gtcatcccgc ggcgaggagc gcgcggtcca ggccgccgaa gccgcgatct cctcgccgct
    27661 gctcgaggcc agcatcgacg gcgcgcacgg tgtcctgctc tcggtccaag gcggcagcga
    27721 cctgggtctg ttcgagatca acgaggccgc ccggctggtg caggaggcgg cacacccgga
    27781 cgccaacatc atcttcggtg cggtcatcga cgacgccctc ggcgacgagg tgcgcgtgac
    27841 ggtgatcgca gccggtttcg acggcggcac cccgcagaag cgcaccgacg agcgggccct
    27901 cggcaccgtc gtctcccgcc ccggcgtccc cgccccggcc gccggcgctc gccccgctcc
    27961 cgcggcccct gcggagtcgg ccggccaggc cgcgccggcc ccgacccccg ccgcatcggg
    28021 tgcccccgcg gccgagccgg ccgacgcgcc ggccgccgag cccgctcctg cccagaccgc
    28081 gcagccggcc cccgccgcag agccggtgcg cccgcccaag caggtcgtct tcgacgaccc
    28141 cgaggacctc gacgtccccg agtggctgaa gtaacccgtc aggccccggc gtgttcgcct
    28201 ggctcgacga gggctcccgg gtcgtgcggg ggttcaccga ccggcacgac ggccacagcg
    28261 ccggcgccca cgccggcctg aacctcggct cccgcgtcgc tgacgatccg gcggcggtgg
    28321 cgcgcaatcg cgccgccgcc gcggcggcgt tgggggtgcc cgccggcaac ctcgtcttcc
    28381 tccaccaggt ccacggcgcg caggtggcga ccgtgaccga accatgggga ccggaggacg
    28441 agccggaggc cgacgccctg gtcaccaccg tgcccggcct ggcgctcgtc gtcctcgcgg
    28501 cagactgcgt gcccgtgctg ctcgccgacc ctgtcgaggg ggtcatcggt gcggcccacg
    28561 tgggccgccc ggggctgctc gccgggaccg tggcggccgt ggtcgagcgc atgcgttccc
    28621 ttggtgccgt ggacctcgat gcggccgtcg gccctgcggt gtgcgggtcg tgttacgagg
    28681 tcccgcagca gatgcacgac gatgccgtcg cgctggtccc cgcggcgcgg gccgtcagtg
    28741 ccaccggcac cccggccctc gacatcgcca cgggggtgct ggcgcagctc tcggccgccg
    28801 gcgtcagcgc ccggcaggtg ctcggctgca cccgcgagtc cggcagcctc tactcctatc
    28861 gccgcgacgg cgtcaccggc cgccacgccg gcatcatcct gctgcggccg ccggctgccc
    28921 accgcccggg acgacaccac ccgggatagc gcgacgggct cgctccacgg aagcgttgcc
    28981 ccgacattcg gcgcaccgcg acattcggaa tacgagtcgg gccggttgac cggtcattcc
    29041 cggggcttcg agcgctcctc gtagttcgga tgtcgcagcg ctgtccgccg gccctcccat
    29101 ccaggaagat ggggggtatg tctgccgcgc gtgccgatgc tgttgccgcc cgtcccgagc
    29161 gggtcgagga gatccgggcc gggttggccg ccgtcgagga gcgcatcacg gccgcgtgcg
    29221 ccgcggccgg ccgggctcgg gaggaggtca cgctcatcgt cgtgaccaag accttccccg
    29281 cgagcgacat ccggatcctc gccgggctcg gggtgcgcga tgtgggggag aacaaggttc
    29341 aggaggcgca cgcgaaggcg gaggcggcgt catacgacgg cctgcggctg cacctgatcg
    29401 ggcagttgca gcgcaacaag gcggccgagg cggcgcgcct cgccgacacc gtccactccc
    29461 tcgaccggcc caaactcgtt gcggcacttg cccatgggcg cgagcgtgcc ggccttgcgg
    29521 agccgcttcg cgtgctcatc cagatcagcc tcgacggcga tcccgaacgc ggtggcgcgc
    29581 cccgcgacac catcccggcg ctggcggagg cagtcctggc ccagccactg ctgcgcctcg
    29641 gtggcgtgat ggcggtgccg ccactggcgg ccgacccggc cgcggccttc gccgacctcg
    29701 ccggcctggc cgcggcggtg cgccgcagcg ccccggaggc ggactggatc tccgccggga
    29761 tgagcgcaga tctcgaggcg gcggtggccg cgggggcgac acacctgcgt gtcgggaccg
    29821 caatcctcgg ttctcgggcg tcacttcgtt aacttcaatg gcgaagagtc tccggcgggc
    29881 gccaccgcaa gcacgtcggg aggaaataga gagtgaggga tcgacgggca tggccggagc
    29941 gctgaagaag acgatggtct acctcgggtt cgccgaggac gatcgatatg acgactacta
    30001 cgacgccgag gcttacgacg acgagcccac cgaggctgct ccggcgcgtt cggagcgcca
    30061 cgaggtccgg caggacgtgc ggccggagcg ctcggccgag gtgacaccca tgccgcggcg
    30121 ggccacgttg agccaggtcg tccgggagga gcgcgcgtcc accttgagcc gcatcacgac
    30181 gatccacccg cgcacctaca acgaggccaa gaacatcggc gaggccttcc gcgagggcgt
    30241 gccggtgatc atgaacctct ccgacatggg cgacgccgac gccaagcggt tggtcgactt
    30301 cgctgccgga ctggtcttcg gcctgcacgg gtccatcgaa cgcgtgaccg ccaaggtgtt
    30361 cctgctctca ccggagcacg tcgaggtggc cacggatgag agcgccatcg aaggccctcg
    30421 cgagctgttc aaccagagct agtccgaggc tggcgcggcg cgcgcggaat cattggccgc
    30481 gctcggacgt ctaatgccgt atgacgcttc gccctaccgc ttccggagcc tgacgtgagc
    30541 ttggtccgag agatcctctc caccgcactc ttcctctatt tcctgctcct gatcggccgg
    30601 ctcgtcttcg agtggatcca ggtgttcgcc cgcgactggc gcccgcgcgg cccggtgctg
    30661 gtggtcgccg agggcatcta taccgccacc gatccgccgt tgaaagccct gcggcgggtg
    30721 ctgccgaccc tgagattcgg caatgtcggg atcgacctgt cgttcctcgt gctcatgatc
    30781 gggatctccg tgctgatgtc gatcctctaa gctgaccgac tgatcttccg ttcgcttccc
    30841 gatcggccat gtcaggagac ccgatgccgc tcacacccga cgacgtcctc aacaagacct
    30901 tcgcgacgac ccagttccgc cgagggtatg acgagcgcga ggtcgacgac ttcctggacg
    30961 agatcgtggc caccctgcgg cgccaggaga aggaactacg agagtgccgg ggcagcgagc
    31021 cggtccagga cgcggccagt gccgtgccgg tcatggacgc ttccgacggc cggctcgagc
    31081 gcgaggtcgc actccgggaa gaggccgaac gcgcgcttgc cgacctccag aaacgcatgc
    31141 gcaagggtga cgaggagcgg atcgctgcgg cagccgcctc cggcagcccc gaggaggccg
    31201 acctggctca gcgcatcctc gacgcgaaca gccaggccga cgccgccgag caggacgccc
    31261 gggcccggat cgaggctgcc aacgcgcgcg ccgacgaagc cgagcgggcc gcccaggagc
    31321 ggatcgaccg ctacaccgcg gacgccgacc aggccgaagc caacgcccag gaacgcatcg
    31381 ccaccctctc cgcccgggcc gaggaggcgc agggcagcgt gaccacgccg agcaccggcg
    31441 acgcgaccgg cctgatcgcc ctggcgcagc gggtccacga cgagcacgtc gctgagggtg
    31501 aggccactcg ggcgcgcctc atcgacgaag ggcagtcgca gcacgacgag ttgatcgcga
    31561 ccgctcagtc gcaacacgac gagctcgtgt ccagcgcgca ggcacggcac gacgagctgc
    31621 ttgcgaccgc tcagtcgcag catgacgatc tcgtctcgac ggcccaggcc cggcacgacg
    31681 aactcctcac caccggccag tcgcagcacg acaccctcat cggtgacgcc acgacggagc
    31741 gggactcgat cctgaccggt gcgcgcgagg aggcagcgag cctgctcgcg gcggcgaacg
    31801 ccgagcggga ccaggtcatc ggcgacttgg acggccagcg cagcaagatc gaggagctga
    31861 tcgcttcgct gcgaggtcac gagcagaact accggctcgg gctgcgcacc cacatcgaaa
    31921 cggcgctggc cgacctcgac gccgcgccgt cggtggagga ggcacttgcg gcgccgggcg
    31981 cggagagtct cgcagccccc gaggcggacg gtctcgacgc gtccgaggcg gacggtctcg
    32041 acacgtcaga ggtggacttc gaggaggaga gctccaagga cgcggccgca aactgatccg
    32101 tcgcggcggg ccgcctgcgc cgtacggggc gtatgcccta ttctcagcca ccgagcgtcg
    32161 cgtcagcggc gcttcggaca tcgaggccga aaggggcctg cgcttgacgc cacgacaggg
    32221 cacgacgacg gccgcggcga ccagcgcgcc cccgcgtggc ggcaggacgg ccgcgacccc
    32281 tccgaacggt aaggccgtcc cgtccggcaa gaaggccgcg gcgaaacagt cgaccgccac
    32341 taccgcggcg aaggccgccg ccaccaaggc gcctgcttcc aaggctcctg ctgccaagcg
    32401 ggcgccggcg accaaggccg ctgccgccaa ggccgctgcg gggaaggagg ccgcggcgac
    32461 caaggcaacc ggaacaaagg ctgctgccgc caaggctgct gcnnnnnnnn nnnnnnnnnn
    32521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32581 nnnnnnnnnn nnnnnnnnnn nngcgaccaa ggctgcgacc gagaaggctc cggccaagaa
    32641 ggcagcgaca aacaaggctc cggccaagcg cgcggcgaaa gcagccccgg ctgcgccgcc
    32701 cgggggcggg tcgcggcata ccggcaccgg ccagcggtat gccgtcaagg agggtgagca
    32761 gccgtggacg gcggcggaga tcgccgagat ccgcgccacc atcgaaggcg atatcgcgac
    32821 cttgcggggc gagatcaccg aggccgagca cgaggtcgcc gcgctgatgc ggcagtccgg
    32881 cgacggcgcg ggtgacgacc aggccgacgc gggcagcaag acgctcgaac gcgagcagga
    32941 gatgagcctg gccaacaacg cccgcgagat gctcctgcag gaccagcacg cgatcgaacg
    33001 catcgacgac gggacctacg ggatctgcga gaactgcggg cagcccatcg gcaagctgcg
    33061 gctcgaagcc aatccacgtg cgaccctatg tgtgccatgc aagagcaaac aggaacgccg
    33121 ctgacgcacg aggacccgac cggcgagcac ccggcccgac cgcataccgg aaggagacgg
    33181 cgggccgccc tcgtctgggt ggtcgccctc gccaccctcg ccgccgacca ggcgacgaaa
    33241 ctgtgggcac tgcataccct gacgcccggc gtgagcaagc ccctcatcgg cgacctgatc
    33301 gggttgcggc tcgtccgcaa cgcgggggcc gccttctcga gcctcgacac cgccaccatc
    33361 gtcgtgacgc tggtcgcgct gctcatcgtc gccgggctcc tctactacgt gcacaccagg
    33421 gcgctgtcgc gtgtctcggc gctctgcctg gggctcatcg ccggcggggc gctcggcaac
    33481 ctgatcgacc gggcggcccg ggagccgggc ttcctgcggg gccacgtcgt cgacttcatc
    33541 gactacttcg gctggttcgt gggcaatgtc gccgatatcg ccatcgtcgt cgcggcgctg
    33601 gtcctggtgg ccgtgtcctg gcgctcggag cgctcggatg gctgagacga agacggtgct
    33661 ggtccccgag gggttggccg gcgagcgcgt ggacgccgcc gtcgcccgcc tgctcgggct
    33721 ctcgcgctcg aaggccgcgg acctcgcggg tggcggcgcg atcctcgtca actccgcggc
    33781 ggtggccaag tcggcgaagg tcgtcgcggg cgacctcctc gaggtgacga tcccgtcccc
    33841 gaccggggga ccacgcgtca taccgatgga catccccgac ctgcggatcg tccacgccga
    33901 cgacgacctg atcgtcgtcg acaagcccgc gggggtcgca gcgcacccga gcgtcgggtg
    33961 ggacggaccg gacgtgctcg gcgggctgct cgcggccggc tatcagatca ccacctccgg
    34021 cgcggcggag cggcagggca tcgtctcccg gctcgacgtc ggcacctccg ggctgatggt
    34081 ggtcgccaag agtgaatacg cctactcgcg gctgaaacag gccttccgca cgcgcacggt
    34141 cgacaagacc tatcacacgc tcgtccaggg cttgccggac ccgctcgtcg ggaccatcga
    34201 cgcacctatc gggcggcatc cgtcctacga ctacaagttc gcggtgatgg attccgggcg
    34261 cgcgtcggtg actcactacg cactgatcga ggccttccgc gcggcggcgc tcttggagat
    34321 ccatctcgag accggacgca cccaccagat ccgggtgcac ttctcggcgc tgcggcaccc
    34381 gtgcgtgggg gacctgacgt acggcgcgga ccccgtcctc gccaagcggc tcggcctgga
    34441 gcgccagtgg ctgcatgccg tggggctggg tttcgagcac cccggcacgg gggagtatgt
    34501 gagcttcgag agtccctacg cgcccgacct cgaactggcg ctggagaagc tggcgggctg
    34561 agatgggtgc tcggcccctc cgccgtcggg cagtccgccg gcgcgaggcg gacggctggc
    34621 gcctcgcccc cttcgtcagc tcggctggtc gtcccgggcc agggtgacga tgacggtatg
    34681 ccgtcgaccg tcgtcgcgca ggggcagcac ctgggggtcg ggcacgggca cgccgtccat
    34741 cgtcgtcgtg gccacccggc gccgcacgcc aggggcgccg ggcggggcgc tgaactgcac
    34801 ggcatactgc gccgcgccca cccggatgtc ggcctgcgcg ctcgtccacg acggcggcag
    34861 gcagggccgg atgagcaacc ggtcgccccg gcgctgcagc ccgaggatcg cctccacggc
    34921 ggcccggtag agccaggcgc tcgacccggt ataccaggtc cagccgccgc gccccacgtg
    34981 cggctggacg gagtagacgt cggcggccac gacatacggc tcgacgcggt aggtctcgat
    35041 ccgttccggg cccgagccgt gccagaccgg gttgatcagg tcgaacagct caccggcctt
    35101 gtcctcccaa ccctgctccg catacgcgaa gatcagccag ctcgccccgt gggtgtactg
    35161 gccgccgttc tcgcggatgc ccggcgggta ggagcggaca tatcccgggt ccggttcgga
    35221 ggtgtcgaac ggcggggtga acagccgggc gatgccctcc tcgtgcatga ccagacgacg
    35281 gtccacctcg cgcagcgcct ccgcggcccg gtccgggtcg ccctgcccgg acaaggtggc
    35341 ccagctctgg gcgatggagt cgatctggca ctcgggccgg gtcaccgagc ccagtggggt
    35401 gccgtcgtcg aaatagcccc gccggtacca ctgcccgtcc caggcctccc gctccagcgc
    35461 ctgccgcacc tcctcggcgg cctgccggca gcgggcgacg aaggcgaagt cgcgccgccc
    35521 ccgggccaac gggaggaaac cgtcgagggt atgcagcagg aaccagccca gccagacgct
    35581 ctcgccccgg ccgtgcacac ccacgcggtt catcccgtcg ttccagtcgc ccgagcccat
    35641 cagcggcaat ccgtggctgc cggtccgcag aagggcatgt tcgagggcgc gcgtgcagtg
    35701 gacgtagagg gggtcggtgc gtgtggtgac ggtgggggtg aagaagttct cgtgctggcc
    35761 cggttcgagg gccgggccgt ccagccaggg cacctcgaca tccaggatcc ccacgtcccc
    35821 ggtgacggcg acataccgcg tgacagcggt ggcgagccag acgacgtcgt cggtgatgcg
    35881 agtgcggatc cccgccccac cctccggcag ccaccagtgc tggacgtcgc cctcggggaa
    35941 ctgccggccg gcggcccgca gcaggtgctc gcgcgccagg gccgggtcga cgagcacgac
    36001 ggccatcccg tcctgcagct ggtcgcggaa cccgaacgcg ccgctggtct ggtagaagcc
    36061 ggtgcggccc agcacccgcg cggccagcgt ctggtagagc agccagccgt tgaccatcag
    36121 gtcgaaggtg gggtcggggg tgcggacctc gacctggtcc agcatccgcg accaggccgc
    36181 ctcgactgtg gccagctgct gctcggggcg ctcgcggcgc tgccgggcga tggcggcccg
    36241 tgccgcatcc gcgtcggccg cggcgccgag cacgatgcag acctcgaccg tcccgcccgc
    36301 cggcacgtgg acgccggtgc gcaacgccag gcacgggtcg agccccggcc cggtgcggcc
    36361 cgacaggggc tggcgggaca ggacggcggc cggcgcggcg agagtgccct gccgcccgag
    36421 gaactccccg cggtcggccg tgaccgactc ctgcgccccg gcgagatcgg cgaagacgac
    36481 ctgatcggca tactgcaggc tccacggatt gcgcacgaac agggcgccgg tctgggcatc
    36541 ccgggaggag atcagccggc cggcgctgcc gtgccggtgc tggccgagga ccgcctcggc
    36601 gtaggtcgtg atcgtgtacc tgcgggcccg gtcggtcggg ttggtcaccc gtagccggcc
    36661 caccttcagt gtctcgccgt cggggacgaa ctgggtcaac tccaggtgga cgtatgcggt
    36721 gttgtggacg taggtcgtgc ggccgaagcc gtgccggcag aggtgccggc cggtgggtat
    36781 gggcagcgcg gtcggcgtgg cgatccggcc ggtctcgtcg tcgcggacgt agaccgcctc
    36841 ggagggggtg cgggtcacgg tgtcgttgcg ccacggcgtc aacacgttgt cgcgcgcgtt
    36901 gcgataccac gtcgtgccgg ccccctcggc ggtggcgagg aaaccgaact gcggaccggc
    36961 gacgacattg ctccaggggg ccggtggcag ggcgtcgccg tcgatgacgg tgacatattc
    37021 gcggccgtcc ggggcgaatc cgccgtagcc gttccacgcc accagatccg ggcgcggcgt
    37081 ctcgtggacc gggaccggcg tcgccgggcg cgtgcggctc ggacccagcg ccggcgccgc
    37141 ggcgcggggc agctgatcct ccacgtcacc cacgccggcg tccagcacga cggccgcgct
    37201 cgccagcagg gcgttcagcg tggccggatc ggtctggtcg ccgcgcacca cgaagatccg
    37261 cccggtggag tcggccgatc cggtgcgggc ccggatcgag ccggccaggc tctccagggc
    37321 caggtgcaga tcctgggcgt aggaggtcgg ctccaggttg agcaggacca cgtcgacggc
    37381 gaatcggcgc aaccgccagt attcgaaggc ccggatgagt tgctgcacca ccgggacgtg
    37441 gctggtgtcc gggatgcgca ggacgacgat cggcaggtcc ccggacaccc cgagcggcca
    37501 gagggcggac tgcggggcgg cgtcgcgggc gaggtcggcc gggctgggac ggaggccggc
    37561 gatcgggtag gtgatgcgcc cggcgagagt ctggaacacc ccggcctcgc gggtgctgat
    37621 gcccaggtgc cgcaactcga cctggctctg ggtccacgcc agcatggcga gccgggggac
    37681 ggcggtcggg gccgaatgcc ggtccatgag ggccagcagg tcctcccggg tggatgcggc
    37741 gaagctccag atcgtgacct gggcctcgcc gtcgccgggt atgcggacgc gctgctggag
    37801 ggagaggacg ggatcgatca cgtgcccggt ccggccactg gcggcgccgc cccggtgcag
    37861 ttggccggcc tcccggatgg tgcggccgcg gccgaggaag gtggcgcgat cggtctcgac
    37921 gctcagcgcg ccgatcgcgg cctgcccctc gacacggatc atctccccga accacacctc
    37981 cggctcggac gggttgcgcc gccgccggtg ggcgatgacc gcgccccgtt cgggcaggaa
    38041 ttcggtcgcg acgaacagct tggcgaacgc cgggtgcgca tcgtcgtcgc gggccggggc
    38101 gaggacgggc tcggcgtagg tcagcagggt taggcggcgt tccttccgct gccggttgtg
    38161 gatcgtcagg tgccgcaccg cggcgtcggc ctccggggag aggtggtagg tcagccgggt
    38221 cgtgaagcgg tgatggttgc gggtgaaagt gaccttgtcg tcggccatcc ggacctcgac
    38281 gctgtgggca ccggccagga tcggcagcgg cgtcgccgac cagtagtcgc cctcggcctc
    38341 gtcgcgcagg aagacgtgcg agccggcttc ctccgacgtc gggtcggggt gccagcgggt
    38401 cagggccacg ccttgccagc gcaggtgccc gcccccggcg ggggtcaggg tcaggctcag
    38461 gctcccgttg gacatgtggt ggaccgcggg ttcgtatgcc gccgggccgg tgaagacgcg
    38521 ttcctccggg gcagtcaggg cccgcagcac gcgtgcctgg ccggtctcgc gccggtggtg
    38581 ggtgaccggg acatcggtcg gcgcctgctc ctgcaggagc aggtcaccgg agcgcaccag
    38641 cggctcggcg tggaaatagt cccgcagcgc accctgctgc acgacgttgt ggatcgccag
    38701 gatcgacatc gcctggtggt gggccatgtg gcagcgcacg accgcgtggc cggcgccctc
    38761 gggtagccgc gccggcgtgt agtccaccga ctcgtagaac ccgaaccggc ccagcgcccc
    38821 cacgccgtcc aaccgtccga agttcgtcag cgcggcaccg ggatcgatca tcgccgctag
    38881 ggccgtcgcg tagggcgcga tgacgaggtt gtcggcgagg ccgcgcatga tgccgagacc
    38941 ggggacgccg aagggggagt actgataggt ataggcgaca tcgcgcgcgt tgtaggcggc
    39001 ctcggagatt ccccacggga tccccgagtc ggaggtgtac tccatctgtc ggcgcaccac
    39061 gagccggttg gtcttggcca gcagtccctg atagggcgaa cgcagcagca gcggcggcat
    39121 gaggtactcg aacatcgagc ccgaccacga ctgcagggca gcaccacccc cgaccgcggc
    39181 gaccggccgc ccgaggagga accagtgccg ggtgcggaca tcgcgtttga tgaccgcgac
    39241 atagctcgcc agccgggcct cggaggccag caggtcgtag cacgactcgt ccagccgccc
    39301 ggtggggatg tggaagccga cggagagcag gccgcgccgg tcgtcgcgca ggaatccgaa
    39361 gtccatctcg tcgaagagtt gccgcgacgc cgcagccacg gcgcgaaggc ggccggcgag
    39421 cttctcccgg ctgctggggt ccaaggcggt gtccgcgccg agactgtgcg cgctcgccgc
    39481 cacctggccg gcccacaggc gggtctcggc atacaccggc tcgtcgggca accgctcgca
    39541 cgccgccacg agtgcggcgc acgcggcctc gaccggcgcc caggcgggct gggtctggcc
    39601 ggccggcggg ggtgtggcca gcgcggcata cacgtgcttg gccgcggcgg cgatggcctc
    39661 ccgcgccggg tcctcgatcg cggtcagcgt ggtggacagc acgtcgagcg cgtcggtcaa
    39721 tcccgcggga tccggctccg gcgctgcgcg caagagagtg tccgaccagg cgcgggtcgc
    39781 gctggagagc gtcagcaggc aggccgcgag gttgccgctg tcgaccgtgg agatatagcg
    39841 cggttcgagc gcgcgcaagg tcgtcgtctc cagccagttg acgatgtgtc cgcggtgccg
    39901 gtccaggcgt tcgagggtgc cgagagtgcg ttcgagacgt tcggtggcgt cggcggtgcc
    39961 gatccagccg agatcgcgcg cgctgacgat gcacagcagg tacaggccga tgttggtcgg
    40021 cgaggtccgg tgcgcgacaa cgggttcggg gtcctcctgg aagttgtcgg gcggcaggtg
    40081 gttgtccgcc tggccgacga aggtgtcgaa gaacagccag gtgcggcggg cgatccggcg
    40141 caactgggcc cgctcgacgg ggctggcctc gagttgcagt tctcggtgcg ggcggctggc
    40201 ggcggcggcg acggcggggg cgaggatcca cgccagggcg ggcacggcgg cgacggcggc
    40261 ggccgccggc ccgcgccaca ggccggtgcc cagcacggcg gcggcgggga cgaggccgcc
    40321 ggccatctgc cgggcgtatg ccgcgccccc ctcccggcta cggcgggcgg cggcggccgc
    40381 cgtcgtccat tcgagcaggt ggcggtgggt gacgaggagc cgggtcaggg tgcgggcgat
    40441 ggcgtcgatc gacagccagg cctgatggcc gagcaggacg aggttcatcc cgccgagcag
    40501 gacgccttcg ccggcctcgc ctagatgatt gattccaagg tgactggtgt ggcgtatgtg
    40561 actcgggggt gagggtgccc agactcggtg ttgcgaagca actcgagctc tggaggctc
//
LOCUS       DAOPJG010000008        39708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1061_length_39708_cov_7.737573, whole genome shotgun sequence.
ACCESSION   DAOPJG010000008 DAOPJG010000000
VERSION     DAOPJG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 39708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39708
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1061_length_39708_cov_7.737573"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..1057
                     /locus_tag="PKU75_01720"
     CDS             293..1057
                     /locus_tag="PKU75_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HPF79623.1"
                     /translation="MTLWIDPPRWPAHGRLWSHLISDASLEELHEFAAAQGIPRRGFE
                     GDHYDVPQERYAAIVAAGAREASGPDLLRHLQASGLRLRKRKGEKGIARIHGVVFPDG
                     SRADVDLVRSSHEPPDHSVFAATVYVCDAEGSWLVVWSPRRQEWSAPGGWREQGESPI
                     ETAVRETLEETEIRLRPDDLSMVAYERFYPLDGAPWAVPAGRFLTVYRTQLTVIRPEI
                     LAAQDEPARWVTTKEFMELARTAWWLPIAQAIANRE"
     gene            complement(1059..1376)
                     /locus_tag="PKU75_01725"
     CDS             complement(1059..1376)
                     /locus_tag="PKU75_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79624.1"
                     /translation="MQVELTGGATGRVSGYADQLVRAWGAGRKAEALKYGTSGVVNQL
                     WDFSHGAGGGGWIRTTTVGGMFETKVSYTCESAYVDFTIKPAAAAAGQPHSVVKVYTQ
                     GAD"
     gene            complement(1521..2171)
                     /locus_tag="PKU75_01730"
     CDS             complement(1521..2171)
                     /locus_tag="PKU75_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79625.1"
                     /translation="MPTVTPDRAFGHDVHELSDAPDGAAIGAAALRLWQRYRETHRHY
                     HDLRHLDEVLAAIGEIATAATEIDLARVRLAACYHDAVYDPAATDNEARSAALAGHEL
                     SILGVPDGHLDVICRLIIETADHALPPSGSPSAWLHDADLWILAAPPERFDQYCRAVR
                     AEYAAVPTDAYATGRSAILTEFLDRPALYAEPSARHDWEPLARSNIAREIARLDIR"
     gene            2418..3185
                     /locus_tag="PKU75_01735"
     CDS             2418..3185
                     /locus_tag="PKU75_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79626.1"
                     /translation="MTMPITPGAPAVVAARTEGLAKVYGRGDAQVVALGGVSVALNAH
                     EFTAIMGPSGSGKSTLMHCAAGLDAVTEGKVFVGDVELTALKEKELTALRRDKIGFIF
                     QAFNLIPTLTAEENILLPLSIAGRKPEQAWFDRVIDTVGLRERLGHRPSELSGGQQQR
                     VACARALVSRPEIIFADEPTGNLDSRSSREVLDFLRRSVTEFGQTIVMVTHDPVAAGF
                     TDRVLFLADGQVVDEMRNPSADRVLERMAHLDSAAGV"
     gene            3192..5732
                     /locus_tag="PKU75_01740"
     CDS             3192..5732
                     /locus_tag="PKU75_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPF79627.1"
                     /translation="MLRASIKSVLGRKLRLLMSAFAVILGVAFVAGSFIFTDTLGKSF
                     DGIMNSSVGDVIVRPAGTEDSFGSSQTLPGDLVAELKDVPGARAVDGNLTDYTTFVVS
                     KKGKLIGGQGAPGLGVNFNTAPSAGGESMFRLAEGAWPAKDGEIAVDSRTAKTGGYVV
                     GDTVQVVTAAAQAKVAPKLTGIMEPSGSLVGASVVVWTTHQAQALYQEGKDVYNDVWV
                     TAADGTSQDELQTAVAKVLPPDLEAVTGEKAAEAMRTSIQDSMKFITYFLLIFAAIAL
                     VVGTFIIVNTFSILVAQRMKELALFRAIGASRQQVARSVLVEAFIVGLVGSTVGLLVG
                     IGLAIAIKALFGSIGLDLSDASLVIAPRTVLASYLVGLIVTLLAAYLPARRAGKVPPV
                     AAMRDDVTLAETSMHRRVIAGAALAIAGAIAMGLGLFVDVPNAIVWIGIGILGVLLGV
                     AGTSPLVGRPLITGMGRLYRRMFGAVGVMAEENALRNPRRTAATASALMIGVTLVSMM
                     AVFGSSAKQSINGEIERNFKADYLVSNAIGSDFSPSVGDEIAKIPGVETVSRMKWGMG
                     KVKNSDEWIAAVDPATFGSVSTIDEKNDATVLKQLTAGGLLIDSEKASKLGVKAGDMV
                     PVTLQGKPFDVKVAGTFESSATAPSGLIVDLATWTQHGGTAADGQVFVKAAEGADMAA
                     VTSAIDKVVADLPTVSVKDMAGYAEEQSKPIDQMLMIIYALLGLAVIIAILGIINTLA
                     LSVIERTREIGLLRAVGLGRGQLRRMLRLESVVIAILGAVLGIVLGVVFGIAIQRALA
                     DQGFTALSIPWLQLIAFLLLAGLVGVLASVWPGRRAAKMQILDAISSE"
     gene            complement(5806..>7375)
                     /locus_tag="PKU75_01745"
     CDS             complement(5806..>7375)
                     /locus_tag="PKU75_01745"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HPF79628.1"
                     /translation="RASWWEREGMVVRLLGVAGAGVLLIGVALLVTYAIQHGYLGPVA
                     RVTGAGILAAGLIAFAGRVHRDPQRASGALAIASAGYATAYLDVMAVTTIYDWLPAPA
                     GLVLAAVIGASGIILARAWNSQFLALVTVGGVAALAPVIGSGRTLLTAAFLVVLAITA
                     YPAHIGHSWFVLHAVRVLPAVAALLAVLPRQDQHADVVALAAVLAGFELGTGLMATRR
                     PEPTLLAAVLPLSALPLFATADRLPVPAWLIFSAAALVYSLAAVAARGEHVRDVFRLA
                     PWAGAVGTAFAAAAVDAGTSPSWRTSAIALLALAYAASARGLGTATPSWHTMSAIVLT
                     TAAVVAALPTAMLMASRPYAVTTAFVDHVVTFAAVACAVAFLRGPLHRYAARLPHENA
                     RLLSLLTIGLGLAFAVATVVAGAVVLGRRIGTAEGGFVTGQALVTLGIAVGAAYLLMH
                     GLRRSGDAGLALRAGLALAAVAVGKLLFYDLAALDGIVRIAAFIGAGLALLAMGTGYA
                     KALERSRTAPTPLS"
     assembly_gap    7376..7475
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7476..7732)
                     /locus_tag="PKU75_01750"
     CDS             complement(<7476..7732)
                     /locus_tag="PKU75_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79629.1"
                     /translation="MAATQLDSVRRLEAEFAEAMTRMYAVGNGLAALRIDLTAESLTA
                     QSRRGPSPAAAVAPLGEATARPATTAPERRPTQQPVTPPRRP"
     gene            complement(7805..8845)
                     /locus_tag="PKU75_01755"
     CDS             complement(7805..8845)
                     /locus_tag="PKU75_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPF79630.1"
                     /translation="MRALQYTSVAGPVHVVDLPVPAPEQDGALISVTRTGLCRSDWHG
                     WQGHDSDTVLPCVPGHEFTGTVVEAPADPGLVGSRVVVPFVMACGRCPECVRGSAQIC
                     RAQRQPGFTDPGSFAEFVAVPAAAVNVVPLPDEVTDDAAAALGCRVATAWRALTAVAQ
                     VEPGESVLILGAGGVGLAAVQIAQALGATPIAADISAHALSIAESLGAQTVSLTPHDS
                     EEEIAGQVRTAYPEGVDVSVDALGSPALAAAGVLALRRGGRHVQVGLLDAPRTPLPLG
                     RIIAHEIALLGSHGMAASDYPALLDAVAAGRLDPAALVTATLDLAQAATALVTMTDPG
                     APRGIRLIDPTR"
     gene            complement(8903..10924)
                     /locus_tag="PKU75_01760"
     CDS             complement(8903..10924)
                     /locus_tag="PKU75_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF79631.1"
                     /translation="MPETELEHERAHLKAARTALTRMRERTEALDVAAADPIAAAYLA
                     RTLYYRAQALRDDPGTALFFGRLDTDGGERWYVGRRHVGDEHGDPLVIDWRAEVSGAF
                     YRATRTEPMGVVLRRRFGIAGGEITAYEDEHLTDTGESETKSAILAAEIERPRVGPMR
                     DIVATIQPEQDLIVRAGADTSVCVQGAPGTGKTAVGLHRAAWLLYAHRDRLSRQGVLV
                     VGPNRAFLDHIGAVLPALGEVRVGHTTVADMFATRPVKATDTPAVATLKGDPRMAEML
                     RRFVWSHVVAPTEPLVAPRGSRKWRVPAYEVGDLVDELRGRGARYHAAREMLPHRLAH
                     AVLVQMERAGDSPDDRVQDAVARSSAVKTYAAALWPVLDPAKLLHRLLSDSDTLAAAA
                     GDLLSPDEQAALLWERPAKTSGSAKWSVADLVLLDELRDLLDRTPSLGHVIVDEAQDL
                     SPMQLRAVGRRCTTGSVTVLGDIAQGTTPWATGSWNEALRHLGKTDAHIEVLDRGFRV
                     PASVIRYAARLLPRIAPGLGAPQSVRDNPGRLDVHAVTAGEELDRLVALAISVTAAPG
                     SVGLIVPDARVAEASAALGRAGLEHGVLGVDHGDVNHQIDIVPASVAKGLEFDRVVVL
                     EPAQIVAAEPDERTGLRRLYVALTRAVSELDVIHSTPLPADLTATSPTA"
     gene            complement(11113..12471)
                     /locus_tag="PKU75_01765"
     CDS             complement(11113..12471)
                     /locus_tag="PKU75_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPF79632.1"
                     /translation="MSEPKTPTETSPSPSSDPWAATSTPDPDATAPLWFGERTEQPRY
                     PSSQPAPAPASWSTPGPAPAVTQAPPSPPAGRKRTWPGLVAVSVLSAALASGGTYAAV
                     RAADDGGSSSATTSAASQNSSASASGSGTQSIRQVNPNAPDWTATAKAVTPSVVAISL
                     QVQGGEAQGSGVVIDDAGHVLTNNHVVDGAEQGTLQVTLSDGRIYEATVAGTDPSTDL
                     AVVTITNPPTELTPIALGDSSALAVGDPVMAVGNPLGLASTVTTGIVSALNRPVTTGD
                     QSTQFGQQSDPVYTNAIQTNAAINPGNSGGALVNADGQLVGINSSIATAGSNSGNIGI
                     GFAIPVNEAKAIADQLIEKGSATHAYLGVSLRDGEGKDGTATRAGALVAQVSPGTPAA
                     TAGLKDGDLIIGIKGVGVESMDSLIAHIRAEKVGNTVTLKVLRNGAAQDISVTLAAKP
                     AS"
     gene            complement(12545..13966)
                     /locus_tag="PKU75_01770"
     CDS             complement(12545..13966)
                     /locus_tag="PKU75_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPF79633.1"
                     /translation="MTTLAARLHRLPLRWRLIAILLTLLLAALTLTSAATAFLMKREL
                     ISRVDDNLRKSSASIANRAVDVLQGTTDRMPTGYAVVIIPTDGSRPTAIPATDVDAYP
                     AISDLSLEDSRVVTGTPFTLGTDGSTLQWRAVAKASQGGDAIAVVAQPLADIHKTVWR
                     LVQMAVLIGLLTIAVSAVLAWFAVARAFRPLTKIEDTAAAIAAGDLSQRAPESDAPHE
                     VASLSRSFNAMLTHVEESFDVRAASQERMRDFVADASHELRTPLATVRGYAELYRQGA
                     VPATAVPETMARIESEAARMSNLVDDLLLLARLDEARPVEFAPLDLTVVAAEAVAAAR
                     VRDPERRIVVQPLAGALSPTLVHGEEAGMHQVLGNLLTNAIRHTPPGTPVEVVVGRRD
                     DRAVVEVRDHGPGVEEATRTKVFERFFREDKARSRANGGTGLGLAIVAAIVGRHKGRV
                     GIANTPGGGATFVVELPAAPTSS"
     gene            complement(13963..14667)
                     /locus_tag="PKU75_01775"
     CDS             complement(13963..14667)
                     /locus_tag="PKU75_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF79634.1"
                     /translation="MTIAEPEARLLIVEDEPNIRELLATSLQFAGFEVVTAGDGHEAI
                     TAAAHHDPDLVVLDVMLPDMDGFEVTRKLRERGKRAPIVFVTARDSVDDKIKGLTVGG
                     DDYVTKPFSLEEVIARIRAVLRRTKGEIDDSAMLRFEDLELDEDSHDVRRAGRPIETS
                     PTEFKLLRYLMLNPNRVLSKAQILDHVWDYDFRGESGIVESYISYLRRKIDTEGLPPL
                     IHTKRGVGYVLRKPPA"
     gene            complement(14749..15120)
                     /locus_tag="PKU75_01780"
     CDS             complement(14749..15120)
                     /locus_tag="PKU75_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79635.1"
                     /translation="MIPASGVTGNGYHLVYDGRRPDVSPDGSKVLWAWADYDTPMTPG
                     WRRPTCPVGRCGSLPTACSEGWSGDPVFSPDGKQAISGYGWENSQIESFCVTTGCLNL
                     FYYDVPDDSQWVTSLDWQPLP"
     gene            complement(15412..15870)
                     /locus_tag="PKU75_01785"
     CDS             complement(15412..15870)
                     /locus_tag="PKU75_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79636.1"
                     /translation="MRRTPPPRTGLQPFPPRGMPRSRVQDAPAPDAYRRPGHHVRSAP
                     GRPVYAGPVTTGDPYGSSRYAAARPTVNGSAIALVVLSSLCLFPLFMLTQVPALVFGV
                     RALAAQSDPQRSARLARHGWWAFALGLAALVALFLIAAAALVGVLAAAVG"
     gene            16071..16466
                     /locus_tag="PKU75_01790"
     CDS             16071..16466
                     /locus_tag="PKU75_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPF79637.1"
                     /translation="MPTGKVKWYDAEKGFGFIDADEGEDVFLHANALPAGTTTIKNGT
                     RIEYGVAQGRRGVQAMGVHILDAPPSVQANRRERDRKPTEDMVVIVEDVMQLLDKVSE
                     GLRRGHYPDKKFATVVSHALRGVADNLES"
     gene            16466..17206
                     /locus_tag="PKU75_01795"
     CDS             16466..17206
                     /locus_tag="PKU75_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HPF79638.1"
                     /translation="MAAAVKSDSALLAAVDIALAELNESAEPGTVGDHLGAVMSGERL
                     ATHSFACLAPAYPGWVWTVSVARAPRSKTVTVCEVTLLPGADALLAPEWLPYADRLAP
                     GDIGPGDVTPYVADDPMLVQGFEATGDEDVDRTAFWELGLGRARVLSAEGREAAAARW
                     YEGENGPGAALALKAPAPCATCGFFVPIAGALRSMFGICAGEWSPSDGTVVSIDHGCG
                     AHSEVDMDRPDPEKVPAPIVDEYRLDLA"
     gene            17457..>17765
                     /locus_tag="PKU75_01800"
     CDS             17457..>17765
                     /locus_tag="PKU75_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79639.1"
                     /translation="MTLDIPCFDQDPGGDNFPCQTDSAGLVYYIQSSVDIGDSTQLGT
                     RRVVKTMTEQFDPTHLVVTRTSNPTYTGTGITDIIFQKSSVGFEGSTIGFTWCDDALD
                     G"
     assembly_gap    17766..17865
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17866..18499
                     /locus_tag="PKU75_01805"
     CDS             <17866..18499
                     /locus_tag="PKU75_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79640.1"
                     /translation="MLTLGGCSGQGAVTAEAQEFFPSSTAEDWVTYSDAIVNIHVERE
                     FEIPPSADELEADEGIIGRNVSATVKEVMWRQPSRSDLAIPLSLDLGAGGWVFHGSER
                     KELRFSGSPRLEPGHDYLIALAYTTLHPTGSGTPAWIPIGGQAIVPNDSATIGKGEDI
                     ARPGALAKSLQGRPTSDLVDKIWETAPDPKAKGLLDEPASVRRTAVQAAS"
     gene            complement(18510..18797)
                     /locus_tag="PKU75_01810"
     CDS             complement(18510..18797)
                     /locus_tag="PKU75_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HPF79641.1"
                     /translation="MSTLRRDNGIPAPRAPEPIGFRMDRIVLIGMAVWAVGLIVTLVV
                     PTLHEGSRSWWPWACVAGIVLGAIGYLYVRRGRGNAADAHPQEPANPQPQY"
     gene            18905..20404
                     /locus_tag="PKU75_01815"
     CDS             18905..20404
                     /locus_tag="PKU75_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPF79642.1"
                     /translation="MANSSRATTRITPQDNATGLDRFFQITARGSTLSREIRGGLATF
                     FTMAYIVVLNPLIIGTQEDSTGAFLGGGSAPNLAMVAATTALVAGVMTILMGVVANFP
                     LAMATGLGLNAFVTFGVAKLPEMTWADAMGLVVLEGIIITVLVLTGFRTAVFHAVPPQ
                     LKTAISVGIGLFIAIIGFVDAGFVRKSAGGPLGELGVGGFLAGWPLLVFVLGLFITVA
                     LLVRKVRGAILYGILAATALAIVVEAVGKIGAQTNAAGERVNPTGWGLNVPKLPDSVA
                     QTPDFSLLGDFNLLGSFQAIGFVSAALLIFTLLLADFFDTMGTMVAIGAEAGLNDEEG
                     VPPRSEAILLVDSVGAMAGGAASVSSNTSYIESASGVGEGARTGLASVVTGVMFLLAT
                     FLAPLVSVVPYEAATPALVVVGFLMMQQVKDIDWDDIEIALPAFLTIILMPFTYSIAV
                     GIGAGFVLYVLIKIIKGKAGQVHPLMWITAALFLLYFAIDPVKSVLGVS"
     gene            20512..20943
                     /locus_tag="PKU75_01820"
     CDS             20512..20943
                     /locus_tag="PKU75_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPF79643.1"
                     /translation="MPAASTTQITDLATTLRLACMRISRRVRFESTEAIAPHHFSVLM
                     HLSKGERMPGELAALEKVSAPSMTRTIGGLVDLGYAARSVDADDKRCVRVAITDAGRA
                     VLKDTLRSRNAWMATRVAGLTPREREVLAEASIILERLAAE"
     gene            20940..22223
                     /locus_tag="PKU75_01825"
     CDS             20940..22223
                     /locus_tag="PKU75_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79644.1"
                     /translation="MSPTFASLSVRNYRIWFFGALVSNVGTWMARVAQDWLVLTELTE
                     HSASALGIVTGLQFLPFLLLAPWAGAVVDHFPKRTLLMITQISLGLVSLVTGLLAVTG
                     VAQLWHVYILALMQGVATAFDNPARQAFVSEMVPNERLANAVSLNSASFNAGRLIGPA
                     VAGVIIAAWGTGIALLLNAVTFGAVLLALTSLDPRALTPAPLSKGRGSIREGFDYVRG
                     RPDLKLVMGLVFVLGTFGMNFQITMALMATKVFGKGPTEFGLLGSIMAVGSLAAALWS
                     ARRANPRLRHLLIALVAFTVSATAAALAPTYSLFALTLIPLGLAALSALTTANAMVQL
                     RTTPAMRGRVMALYMAIFMGGTPFGSPLIGWIGDHFGARWTIGIGPLAIGLTLAAVAV
                     YLWRHENVRVSFTRGARPHLRILSGNDVIVPEPAR"
     gene            complement(22398..23531)
                     /gene="serC"
                     /locus_tag="PKU75_01830"
     CDS             complement(22398..23531)
                     /gene="serC"
                     /locus_tag="PKU75_01830"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HPF79645.1"
                     /translation="MTTTALTDLRLPDDLLPADGRFGCGPSKVRPEQVAALSAIGASV
                     LGTSHRQAPVKNLVKAVREGLTTLFELPEGYEVVLGNGGSTAFWDIAAFGLVRERAQH
                     LAFGEFSAKFGSVTSKAPFLGESTIIKAEPGSLATPSAEAGIDVYAWPHNETSTGVMA
                     PVQRVEGADEGALVLIDATSGAGGLPVDIAQSDVYYFAPQKCFASDGGLWLALFSPAA
                     LARVEEITASGRWVPDFYSLPTAIDNSRKDQTYNTPAVATLAMMANQLEWMNGNGGME
                     FTTGRTADSSSRLYSWAEQSDVATPFVADPAARSQVVGTIDFADEIDASAIAKVLRAN
                     GIVDTEPYRKLGRNQLRIAMFPAVDPDDITRLTGAIDYVIERM"
     gene            complement(23604..24752)
                     /locus_tag="PKU75_01835"
     CDS             complement(23604..24752)
                     /locus_tag="PKU75_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HPF79646.1"
                     /translation="MSDSEVKHGLEGVVAFESSIAEPDKEGGALRYRGVDLKELVGRV
                     SFGQVWGLLVDNEMDPGLPPAEPFPLPVHTGDIRVDAQSALAQLAPVWGFKPLLDISP
                     EEARENLARASVMTLSFIAQSAHGQATPMVPQSRVDEGKTIVERFMIRWRGEPDPAHV
                     EAIDAYFISAAEHGMNASTFTARVIASTGADVAACLSGAIGALSGPLHGGAPSRAQHM
                     IEGVEQMGDADKYVADTLARGERLMGFGHRVYRAYDPRAEVLRDTCKRLGAPRYEVAL
                     ELERAAIAALAERYPERKMETNVDYWAAVLLDFADVPGEMMTPLFVSARTAGWAAHIL
                     EQKKTGRLIRPSAKYVGEGPRPLSSVKGYVGRDHNDLPIHALVPDPRV"
     gene            24862..25548
                     /gene="pdxH"
                     /locus_tag="PKU75_01840"
     CDS             24862..25548
                     /gene="pdxH"
                     /locus_tag="PKU75_01840"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HPF79647.1"
                     /translation="MTTPLAPRRDYPGDGLAEDQLAPTPYRQAQLWIEEAVARAETGD
                     DVPEPTSMAVATVDPSGRPDVRVVLMRFFAPEGPGFVTNLNSAKGQQLAANDGVAASL
                     TWPAMFRAIRFRGRAEVLPDELIRDYFASRPWGSRISALASEQSAPIASRAELEQRYA
                     ALAAQYPDTGSPGDVPVPDGWGGYRIVPDEVEFWAGRPSRLHDRIVFSRTGSGSMDHA
                     AAWSVSRRQP"
     gene            25687..26310
                     /locus_tag="PKU75_01845"
     CDS             25687..26310
                     /locus_tag="PKU75_01845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79648.1"
                     /translation="MRRARTPPRCSTCSARTRRTLLTLGLGASAISALRGIRLSVLPP
                     WADHIGLPAAQLSLLVALGALVEIAICYPAGWVMDRRGRVWVAVPTAVLLGLGLMLIP
                     VTGSFGELLAATVVMAVGNGLGSGIVMTLGADTAPRLHRAKYLGGWRLFADIGMSGGP
                     LTVSGLAALAGLASVILGVGGVLAAGWPWRWVGPVDRRRRADERMPA"
     gene            26307..27005
                     /locus_tag="PKU75_01850"
     CDS             26307..27005
                     /locus_tag="PKU75_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HPF79649.1"
                     /translation="MSDLIDTTEMYLKTIFELEEEGIPPLRARIAERLGHSGPTVSQT
                     IARMERDGLVSLAGDRHLEFTASGRERATRVMRKHRLAERLLVDVIGLEWEYVHDEAC
                     RWEHVMSERVERRIVELLRGDHTSPFGNPIPGLDELGATSVREGFRDGLQTLTSVAGP
                     NPTRVTVRRIGEPVQVDHEALNLLTDSGVIPGEAAEVHAADGRVIAVGAGRPPADGVS
                     LPPEISDHVFVALT"
     regulatory      27155..27314
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            27317..28105
                     /locus_tag="PKU75_01855"
     CDS             27317..28105
                     /locus_tag="PKU75_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPF79650.1"
                     /translation="MSQHAPGRHRARRRRRDGLRRTSAVLAATGGMITAFAVSADAVP
                     APAVTTPVHKAPAVKAAAPALTAPSAAAPVKTSYGVLGVKAVAKPKPKPKPAPVVTPV
                     ATTTSATAVVAARTQTAPASRSTTRTSTPSNSSSNSASSTPSTPTTPQVPANGGVLAI
                     AASLSGIPYVYGGSTPAGFDCSGFTSYIFAKVGISLPRTAAGQQAAATPVSNPQPGDL
                     VFFGYPAYHVGIYAGNGMMYDSPRTGLSSGLRAIWSSSATYGRP"
     gene            complement(28432..28965)
                     /locus_tag="PKU75_01860"
     CDS             complement(28432..28965)
                     /locus_tag="PKU75_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPF79651.1"
                     /translation="MGRIEIVEGDITKEKVDVIVNAANSSLLGGGGVDGAIHRAAGPG
                     LLEECKDLRSSTLPDGLPPGDAIATRAYNLKKVKWIVHTVGPNRHAGETDPEVLRRCF
                     ANSLSLARGLRSHSIAIPAIGAGVYGWDHLEVARIAMAEADMEKHKRGGIEKIRFVLF
                     SHDAKETWIDARMEMPD"
     gene            29066..29437
                     /locus_tag="PKU75_01865"
     CDS             29066..29437
                     /locus_tag="PKU75_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPF79652.1"
                     /translation="MADEIYYDKKDQLQIVQNSLLPDETVFAVYDGKEVGTGFIGLTD
                     RRVILQDNSFVGGRVAVTSVPYNKITAVSYASNASVFGRFTEETTVAIHTSHGTYEVL
                     LRGSEKAKHTHDVILHYVVHS"
     gene            complement(29446..30387)
                     /locus_tag="PKU75_01870"
     CDS             complement(29446..30387)
                     /locus_tag="PKU75_01870"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HPF79653.1"
                     /translation="MSAIEGVSQIFDADRWDGIPGFEDLTDLTYHRAKEHGTVRIAFD
                     RPDVLNAFRPHTVDELLRVLEHARTSADVGCVLLTGNGPSEKNGKWAFCTGGDQRIRG
                     RAGYQYETEHESTATGQAAPNPIDQARLGRLHILECQRLIRFMPKVVISLVNGWAAGG
                     GHSLHVVSDLTLASIEHGRFKQTDADVGSFDGGFGSAYLARQVGQKFAREIFFLGQEY
                     SAEDGVRMGTVNRAVPHADLEATGLEWGRLINGKSPTAQRMLKYSFNLIDDGLVGQQL
                     FAGETTRLAYMTDEAQEGRDQFLEKRDPDWSPYPWYY"
     gene            30712..30912
                     /locus_tag="PKU75_01875"
     CDS             30712..30912
                     /locus_tag="PKU75_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79654.1"
                     /translation="MRIASGTVIVLLVALILLGLGTAIVIASWDDKPVAISVLAIMGI
                     GTMAMVNGALQARRYDRDNPRR"
     gene            complement(30968..32617)
                     /locus_tag="PKU75_01880"
     CDS             complement(30968..32617)
                     /locus_tag="PKU75_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HPF79655.1"
                     /translation="MTTTSNPAETEFHLDATGRRIFIGLMLGMFVASISQTIVGPAMP
                     LIVAELGGMEHYSWVATAAMLVSAIVVPVVGKFSDLYGRRGFYIAGLIVFMLGSLVSG
                     LAPNFWTLVAGRAIQGLGMGTVMPLAQTIIGDIIPPRFRGKYQGLMGAVFGVTSVAGP
                     LAGGWITDNLGWRWLFWSSIPVGIAATAVVARFLHLPHQRRNAKIDALGIATLAPALV
                     AILLATSWGGTTYAWSSAVILGLYAVGAVLLAAFVWAESRAAEPILPLRLFKNSIFTL
                     SNVAAFGVAMVMFGAIIYIPVFAQGVIGVNATNSGLILLPLMAGLIIFGIVAGQIITK
                     TGRYKAVMLAGVATLAAGVWLLTRLTHEATSGQLTLAMVVLGVGLGLCMQQYTLLVQN
                     AADFTDMGVATASTQFFRNVGSTVGIAIFGTVMTTGLHDAILRHLSPAAARAAEASGQ
                     EIGAGSVLDPSVMAQLPAPVAEAVRMGLAERLHEVFVLGLPILAAVFVATALIKSVSL
                     RTTNHPSGNEEAQHELLDSMATLSPESIQDEEEERFAHTRR"
     gene            32682..33311
                     /locus_tag="PKU75_01885"
     CDS             32682..33311
                     /locus_tag="PKU75_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPF79656.1"
                     /translation="MTSVADHPVLVDEDANCGLRERKKRETRRAIHRAALELASDSGM
                     DALTVDAIAERAGVSPRTFFNYFPAKDDALVGADPDDDVDRLRRFVASRPDTESPREV
                     VHALALDRVAGLELDRELWDMRRELTQREPAIGLRVLGVYARADRAVVEALTERARAA
                     RALSLDDELAVTVETYAALGAFRAAVRAHLSGSHAATFAELVDRAFAIL"
     gene            33439..33511
                     /locus_tag="PKU75_01890"
     tRNA            33439..33511
                     /locus_tag="PKU75_01890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33472..33474,aa:Arg,seq:cct)
     gene            complement(33513..36515)
                     /locus_tag="PKU75_01895"
     CDS             complement(33513..36515)
                     /locus_tag="PKU75_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HPF79657.1"
                     /translation="MPELVDVRTGAYYDSVTLMQVSKRIATTPGITAAQVAMGTELNL
                     EVLAGMGFTLPDGIGVNDVVVALHGESQEALDSGVAAVDSALAELTAASRSAGTGFGA
                     APEPVTLGGAIAAGDADLALISVPGEHASAEALDAIEAGVSVMIFSDNVAVEDEVALK
                     DAAAARGVLVMGPDCGTAVVSGVALGFANVVAPGPVGIVAASGTGAQQVICLLDAAGV
                     GVSHVLGVGGRDLTSEVAGRSTKQALAMLAADEDTESILVVSKPPAEEVLADLESYAA
                     GLGHPVRWAILGAGRPDLTASVESALSASGVEVPAWPTWGSVEGVVPGGVSGGGSLRG
                     LFCGGTLADEAMLIAGEALGPIRSNIPLSPELALGSDMAADGGHVVIDFGDDGLTQGR
                     AHPMIDPALRHERILAEGSDPTCGVLLLDLVLGYGAHPDPAGELAEAIRAARATAADG
                     GRELPVIVSLVGTQGDPQGLERCASALAEAGATAYLSNAEATRAAVAYLTGTSTPRRD
                     VDSIPSRSQFDPVEAATAAGTGERPGAGSAVALPREPVVATVGVGLFSEALADQAVTV
                     TEARWQPPLAGSDTAALRSVLLDQRREAANKLAFDRMTAAGADFVDVRPASEAVGLEK
                     GTFLHAGPPLDWERASGPMKGALIGAMLFEGMADTPEEAEAKLAAGEGITLEPCHHRD
                     AVGPMAGVVSPSMWMYELHDPVHDNRSWCSLNEGLGKVLRYGAYGPEVIERLQWMNSV
                     LGPLLQQAIRKAERIDIKAIVAQMLQMGDEGHNRNRAGSLMLLRELLPNLITADGSPS
                     DIAEAVRFSGANEHFFLNLGMPACKLATLAAHGIPGSSIVTTMARNGTDFGIRVSGTG
                     EQWFTGPANTPEGLFLGSYGPEDANPDIGDSAITETGGIGGVAMAAAPAIVKFVGGDV
                     PFALQATQRMYAITQGEHPAYQIPILEFRGTPTGIDVTKVVRTGILPQINTGMAGRVA
                     GVGQVGAGLVNPPKECFTAALTALAATS"
     gene            complement(36508..37218)
                     /locus_tag="PKU75_01900"
     CDS             complement(36508..37218)
                     /locus_tag="PKU75_01900"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HPF79658.1"
                     /translation="MPFAARSPLAPPVGAEQLVWHVLAAFPTALYLHHPERGLLPLVT
                     AEALALPTAWRLPDSSTRMRWGVAAGDVIESLDHCILLPAGPVRAVRTWRPARVRRSG
                     SYAVELPPSWAADRLGGGRGLTPEGDDEICGALLVAFAGHDTSIADAVLPQLDRTTAL
                     SASLVRAAAQGYAVPGLVAYADARLSGNRAAAGAARHAVEDIGHTSGPALIRGVEIAI
                     AHRQIHQSPSLTKEPARA"
     gene            37255..38691
                     /locus_tag="PKU75_01905"
     CDS             37255..38691
                     /locus_tag="PKU75_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF79659.1"
                     /translation="MEKVANDSGLGDLDLPGRARTQSDFSLSLDVPDDLNKRGLRELA
                     DQLVAALRREDEVHRSLISTVVAGGSLADLCQALAGMMDAVVMVTTTDGRVIAEAGEA
                     EAVAAAYRCPAFDPTGRFLVERESVGERTGTADWVRRASVRIVGGSGAEGRIVAIADD
                     RPMTGNDIHLLERSATVAALALAREQAVLAVESKYRAEFLRDALSGRAGSSVDAVAHA
                     DSLGWTLGERLVVVVAETDEDDSATERSGEEIRALQQRFARAWTRAVGARDRRAPVMG
                     FSQEVVTLLAIPADATTEKVMATITDLARVVRGDGGGGRRPFSTGVSRPVTGVENLPA
                     AYAEALRAVAVGRQKHGPGAITHFDELGIYRLLSLVPDSDELRQFVDDALGELATDDN
                     PENAGLRTTLTVLIDTNLNVAEASRILFFHYNTLRYRIAKLERMLGPFTTDPQLRLTL
                     ALALKVLEMRGLSQNHQARPPARVSQQG"
     gene            38853..>39708
                     /locus_tag="PKU75_01910"
     CDS             38853..>39708
                     /locus_tag="PKU75_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPF79660.1"
                     /translation="MAFSWTLHGDGNLSGPDDVVAPDERLSWGKTIGLGAQHVVAMFG
                     ATFVFPLIMGLNPQLAIMMSGLATIIFLLIVGGRVPSYLGTSASFVGAVAAIRAQEGA
                     DSSRVTGAILVAGLVLAIIGVLIHFMGSGVLHRVLPPVVTGAVVMLIGFNLAPVVAGI
                     YWPQDQWVALLTMLFTIICAVAFKGFISRISVFLALIFGTLLSWILDKTAGPITSVLG
                     GATEATEHVRWNTSGIGDAAWFGLPAKTMVGPDGMEVAGWHMPSFNAAAILLVLPAVI
                     ALIAENTGH"
BASE COUNT         6004 a        13389 c        13930 g         6185 t
ORIGIN      
        1 catcatcggt cctgccatca tcggtcctgc catcatcggt cctgccattg cctgccaacg
       61 agccttcgat gacggactcg gtgatcgcct ggccggcgcc ctgctgaccg gcgatggtgg
      121 tcttgggtgc gtgtcgtgct cttcatgaag ccctgcgact ggagtgggtg aagctgtcaa
      181 tggcgaagac gatgcggccg gcgtctcggt tggtgtccac ggacggtccg ggagtcgccg
      241 tcatcagaaa ctcacgtggt aacgattggg tcgcagacgg caggatggcc ctgtgaccct
      301 ctggatcgac cccccgcgct ggcctgcgca cggccgcctg tggtcgcact tgatctcgga
      361 tgcttcgctc gaggagctgc acgaattcgc tgcagcgcaa ggcatcccac ggcgggggtt
      421 cgagggtgac cactatgacg tgcctcagga gcggtatgcc gcgatcgtcg ccgctggagc
      481 acgggaggcg agtggcccgg acctgctcag gcacctgcaa gcatcgggtc tgcggctgcg
      541 gaaacggaag ggggagaaag ggatcgctcg catccatggt gtggtgttcc cggacgggtc
      601 tcgcgccgac gtcgatctgg tgcgcagctc gcacgagcct ccggaccact cggtcttcgc
      661 cgcgaccgtc tatgtgtgtg atgccgaggg cagctggctg gtggtgtggt ccccgcgccg
      721 tcaggagtgg agcgcgccgg gaggctggcg cgagcagggg gagtcgccga tcgaaaccgc
      781 tgtgcgggag acgctggagg agacggagat tcggctccgg ccggacgacc tgtccatggt
      841 cgcgtacgag cggttctatc ccctcgatgg cgctccatgg gcggtgcccg ccggccgctt
      901 cctgaccgtc tatcgcacgc agctgaccgt gatccgaccg gaaatccttg cggcacagga
      961 tgaacccgcg cgctgggtca ccaccaagga gttcatggaa ctcgcccgaa ctgcctggtg
     1021 gttgcccatc gcgcaggcca tcgcgaaccg ggaatagctc agtcggcgcc ctgggtgtag
     1081 accttgacga cggaatgggg ttgacccgca gcggcagcgg ctggcttgat ggtgaagtcg
     1141 acgtaggccg actcgcacgt gtagctcacc ttcgtctcga acatcccgcc gaccgtcgtg
     1201 gtgcgaatcc agccgcctcc gccagcgcca tgggagaagt cccaaagctg gttgacgacc
     1261 cctgacgtgc cgtacttcag ggcctcggcc ttgcggcccg cgccccaggc ccggacgagt
     1321 tggtcggcgt acccgctcac ccggcccgtg gcaccgccgg tcagttcgac ctgcacgacc
     1381 gcgtcctgtt ggtactcctg ggactctcac ttctcttgga ctcgaggcgc cttggtgctc
     1441 cgcatgccct cccggacgcg ggatggtgat tcgaggttgt cccttctcat cacgatccgg
     1501 tcacgcaccg gttccagacg tcagcggatg tcgaggcggg cgatttcgcg agcgatattg
     1561 ctgcgcgcca aaggttccca atcatggcga gccgacggct cggcatacag cgcgggccgg
     1621 tccaggaatt cggtcaggat cgcggaccga cctgtggcgt aggcgtcggt cggcaccgcg
     1681 gcatactccg cacgcaccgc gcggcagtac tgatcgaagc gctccggcgg cgcggcgagg
     1741 atccacagat cggcatcgtg cagccaggcg ctcggcgacc ccgacggggg caacgcatgg
     1801 tccgccgtct cgatgatcag gcggcagatg acatcgagat gtccatcggg cacgcccaag
     1861 atggacaact catgacctgc cagcgccgcc gaccgtgctt cattgtcggt ggcggccggg
     1921 tcatagacgg cgtcgtgata gcacgctgcc aatctgactc gggccagatc gatttccgtt
     1981 gcggcggtgg cgatttcgcc gatagcggcc agaacttcgt cgaggtggcg taggtcgtga
     2041 tagtggcggt gggtctcgcg ataacgctgc cacaagcgga gcgcggccgc gccgatcgcc
     2101 gcgccgtcag gggcgtcgct caactcgtgc acatcgtgac cgaaggcacg gtcaggggtg
     2161 accgtcggca tacgatcatc atgccctgcg tgacctcgta gcgacaggct gtctccgaat
     2221 gtgttgcggg acagcatgat gtgcgatcta cggggctgat cgccagccgt gacagtggtc
     2281 gtgggtcaac cgataccctt ggtaccaacg gaaattcgag ggttgcttca gggtcccgtt
     2341 caggggaaag cagcatgcgg cgcaccggtt atcaggctag gttcgggagc atggttgaca
     2401 tagcaggggt aactgagatg accatgccga tcacccccgg agcgccggcc gtcgtggcgg
     2461 cgcggaccga gggcctggcc aaggtctacg gcagaggcga cgcccaagtc gtcgcactcg
     2521 gcggagtgtc ggtggcactc aacgcccacg aattcaccgc catcatgggg ccgtcaggct
     2581 ccggcaagtc gacgctcatg cactgcgccg ccggcctcga cgccgtcacc gaaggcaagg
     2641 tcttcgtcgg tgacgtcgaa ctgaccgcgc tgaaggagaa ggaactcacg gccctgcgcc
     2701 gcgacaagat cggcttcatc ttccaggcgt tcaacctcat accgaccttg accgccgagg
     2761 agaacatcct ccttccgctc tccatcgccg ggcgcaaacc ggagcaggca tggttcgacc
     2821 gggtcatcga caccgtcggg ctgcgcgagc gtctgggtca ccggccgtca gaactctccg
     2881 gcggccagca gcagcgcgtc gcctgcgccc gcgccttggt gagccgcccc gagatcatct
     2941 tcgccgacga gccgaccggc aacctcgact cgcgttccag ccgtgaggtg ctggacttcc
     3001 tgcgccgctc ggtcaccgag ttcggtcaga ccatcgtcat ggtcacccat gatcccgtcg
     3061 cggccggctt caccgaccgg gtgctcttcc tcgccgacgg ccaggtggtc gacgagatgc
     3121 gcaacccgag cgccgaccgg gtcctggagc ggatggccca cctcgactcc gccgcagggg
     3181 tctgatccgc catgctccgc gccagcatca agtcggtgct cggccgcaag ttgcgcctgc
     3241 tcatgagcgc cttcgcggtc atcctcggcg tcgccttcgt cgccgggtcg ttcatcttca
     3301 ccgacaccct cggcaagtcc ttcgacggca tcatgaactc cagcgtcggc gacgtcatcg
     3361 tccgcccggc cggtaccgag gacagcttcg ggtccagcca gacgctaccc ggcgaccttg
     3421 tcgcggaact caaagacgtc cccggcgccc gagctgtcga cggcaatctg accgactaca
     3481 cgaccttcgt cgtcagcaag aagggaaagc tgatcggcgg ccaaggcgcg ccgggactgg
     3541 gcgtgaactt caacaccgcc ccgtcggcgg gcggcgagag catgttcagg ctcgccgaag
     3601 gcgcgtggcc ggccaaggac ggcgagatcg ccgtcgactc gcgcaccgcc aagaccggcg
     3661 ggtacgtcgt cggcgacacg gtccaggtcg tgacggccgc ggcacaggcc aaggtcgccc
     3721 ccaaactgac cggcatcatg gagccgagcg gatcgctggt cggcgccagt gtggtcgtct
     3781 ggacgacaca ccaggcccag gccctctacc aggagggcaa ggacgtctac aacgacgtgt
     3841 gggtgaccgc ggcggacggc acctcgcagg acgagttgca gaccgccgtg gccaaggtgc
     3901 tcccaccgga cctcgaggcc gtcacgggcg agaaggcggc cgaagcgatg cgcacctcga
     3961 tccaggactc gatgaagttc atcacctact tcctgctgat cttcgcagcg atcgccctcg
     4021 tcgtcgggac cttcatcatc gtcaacacct tctccatcct cgtcgcccag cggatgaagg
     4081 agctcgcgct cttccgggcc atcggcgcca gccgtcagca agtggcgcgc tcggtgctcg
     4141 tcgaggcttt catcgtcggt ctcgtcggtt cgacggtcgg cctgctcgtc ggcatcggcc
     4201 tcgccatcgc catcaaggcg ctcttcggtt ccatcggcct ggacctgtcc gatgccagcc
     4261 tcgtcatcgc accccgcaca gtcctggcct cctacctggt ggggctgatc gtcaccctgc
     4321 tcgcggcata cctcccggcg cgacgcgccg gcaaggttcc gccggtggcc gcgatgcgcg
     4381 acgacgtcac cctcgccgag acctccatgc atcgccgcgt catcgccggc gccgcgctgg
     4441 ccatcgccgg cgcgatcgcg atgggcctgg gcctgttcgt cgacgttccc aacgcgatcg
     4501 tctggatcgg aatcggcatc ctcggggtgc tgctcggcgt cgccggcacc agcccgctgg
     4561 tcggccggcc cctcatcacg ggcatgggca ggctctaccg cagaatgttc ggtgcggtcg
     4621 gggtgatggc ggaggagaac gccctgcgca acccgcggcg caccgccgcc acggcgtcgg
     4681 ccctgatgat cggcgtcacg ctcgtgtcga tgatggccgt cttcggatcc tcggccaaac
     4741 aaagcatcaa cggcgagatc gaacgcaact tcaaggcgga ctatctcgtc tcgaacgcca
     4801 tcggctccga cttctcgccc agtgtcggcg acgagatcgc gaagattccc ggagtggaga
     4861 ccgtctcgcg gatgaagtgg ggcatgggca aggtcaagaa cagcgacgag tggatcgccg
     4921 ccgtcgatcc ggccaccttc ggctcggtct ccaccatcga cgaaaagaac gacgccacgg
     4981 tgctgaagca gttgaccgcc ggaggcctgc tcatcgacag cgagaaggcg agcaaattgg
     5041 gcgtcaaggc cggggacatg gtcccggtca cattgcaggg caagcccttc gacgtgaagg
     5101 tcgccggcac cttcgaatcg tcggccacgg cgccgagcgg cctcatcgtc gacttggcga
     5161 cgtggacgca gcacgggggc accgcggcgg acgggcaggt ctttgtcaag gccgccgagg
     5221 gcgccgacat ggctgcagtg acgagcgcca tcgacaaggt cgtcgcggac ctgccgaccg
     5281 tcagtgtcaa ggacatggcc ggctacgcgg aggagcagtc caagccgatc gaccagatgc
     5341 tgatgatcat ctacgccctc ctcgggctcg cggtgatcat cgccatcctc ggcatcatca
     5401 acaccttggc gctgtcggtg atcgagcgca cccgcgaaat cgggctgctg cgggccgtcg
     5461 gcctgggccg cggtcagctg cgccggatgc tccgcctgga gtcggtggtc atcgccatcc
     5521 tgggcgccgt gttggggatc gtccttgggg tggtcttcgg catcgcgatc cagcgggcgt
     5581 tggccgatca gggcttcacg gcattgagca tcccgtggct gcagctgatc gccttcctcc
     5641 tcctcgccgg gctggtcggg gtgcttgcct cggtgtggcc gggccgccgg gcagcgaaga
     5701 tgcagattct cgacgccatc tcctcggagt aggcgcccac caagagagca ccacacggat
     5761 cccccctccg gatccgcgtg gtgctctcgt ccatggggac cccggtcagc tgagcggggt
     5821 gggggcggtg cgcgagcgtt ccagtgcctt ggcgtatccg gtgcccatcg cgagcagggc
     5881 caggccggca ccgatgaacg cggcgatccg cacgatgccg tcgagggcgg ccaggtcgta
     5941 gaagagcaac ttgccgaccg ccacggccgc cagggcgagc ccggcgcgga gggcgaggcc
     6001 ggcgtcgccg gagcgtcgca ggccgtgcat cagcaggtat gccgcgccca ccgcgatgcc
     6061 gagggtcacc agcgcctggc cggtcacgaa accgccctcg gccgtcccga tgcggcgacc
     6121 gaggaccacg gcacccgcga ccaccgtggc gacggcgaag gccagcccga gtccgatcgt
     6181 gagcagactc aggaggcgag cgttctcgtg ggggaggcgg gcggcatacc ggtgcagcgg
     6241 accccgcaag aacgcgacgg cgcaggccac cgccgcgaac gtgacgacgt ggtcgacgaa
     6301 cgccgtcgta acggcgtagg gccggctggc catcagcatg gccgtcggga gcgccgccac
     6361 gacggcagcg gtggtgagga cgatcgcgga catggtgtgc cacgacgggg ttgcagtgcc
     6421 gagtccgcgt gccgaggcgg catacgccag cgcgaggagg gcgatggcac tcgtgcgcca
     6481 cgacggcgag gtgccggcat ccaccgcggc tgcggcgaag gccgtcccga cggcgccggc
     6541 ccagggcgcg aggcggaaga cgtcgcggac gtgttcgccg cgggctgcga cggctgccag
     6601 ggaatagacg agggccgcgg cggagaagat cagccacgcc ggcacgggca gccggtccgc
     6661 agtggcgaac aacgggagcg cgctgagcgg cagcacggcg gcgagcaggg tcggttcggg
     6721 acggcgggta gccatcaggc cggtgccgag ttcgaagccc gcgagcacgg ccgccagcgc
     6781 gacgacatcg gcgtgctggt cctggcgggg caggacggca agcaacgcgg cgacagcggg
     6841 gagcacgcga accgcgtgca ggacgaacca ggagtgcccg atgtgcgccg gataggcggt
     6901 gatcgccagc acgacgagga aggcggcggt cagcagcgtg cggcccgatc cgatgaccgg
     6961 agccagggcg gcgacgccgc cgacggtgac gagggcgagg aactggctgt tccaggcccg
     7021 ggcgagaatg atgccgctcg cgccgatgac ggcggcgagg accaggccgg caggtgccgg
     7081 gagccagtcg tagatcgtcg tcacggccat gacgtcgagg tatgccgtgg catagccggc
     7141 ggatgcgatt gccagggcac cgctcgcgcg ctgggggtcg cgatggaccc ggccggcgaa
     7201 agcgatcagc ccggcggcga ggatgccggc tccggtgacg cgggcgaccg ggccgaggta
     7261 gccgtgctgg atggcatagg tcacgagcag cgccacgccg atgagcagca cgccggcgcc
     7321 cgcgacgccg aggagccgga cgaccatgcc ctcgcgctcc caccacgatg cccgcnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggccg gcgcggtggg gtgaccggct
     7501 gttgtgtcgg acgacgctca ggagcagtgg tcgccggccg ggccgtcgcc tcccccagcg
     7561 gggcgacggc cgcggccggc gacgggccgc gtcgggactg agcggtcagc gactcggcag
     7621 tgaggtcgat gcgcagggcg gcgagcccgt tgcccacggc atacatccgc gtcatcgcct
     7681 cggcgaactc cgcctccagg cgccgtactg agtccaactg tgttgcggcc atggctcaac
     7741 tctggcggcg ccgccgcgcc gccgcctgag tgccgctact caactctcgg aaggcgatac
     7801 ccagtcagcg cgtggggtcg atcagtcgta tgccgcgcgg cgcgcccggg tcggtcatcg
     7861 tcaccagcgc ggtcgcggcc tgggcgaggt cgagggtcgc cgtgacgagg gcggcggggt
     7921 cgagccgccc ggccgcgacc gcgtcgagga gggcggggta gtcgctcgcg gccatgccgt
     7981 gcgagccgag cagggcgatc tcgtgcgcga tgatccggcc gaggggcagc ggcgtccggg
     8041 gggcatcgag caggccgacc tggacgtgcc ggccgccgcg ccggagcgcg aggacaccgg
     8101 cggcggccag ggccgggctc cccagggcgt cgacgctgac gtccacccct tccgggtatg
     8161 cggtccgcac ctgccccgcg atctcctcct cggaatcgtg aggcgtgagc gagacagttt
     8221 gcgccccaag ggattcggcg atgctgagcg catgtgccga gatgtcggcg gcgatgggtg
     8281 tggcgccgag cgcctgggcg atctggacgg cggcgaggcc cacgccgccg gcgccgagga
     8341 tcaggacgga ctctcccggc tcgacctggg cgacggcggt cagggcccgc catgccgtgg
     8401 cgacccggca gccgagggcc gcggccgcgt cgtcagtgac ctcgtccggc agcggcacga
     8461 cgttgacggc ggcggcgggc acggcgacga attcggcgaa cgatccgggg tcagtgaatc
     8521 ccggttgccg ctgggcgcga cagatctgtg ccgacccgcg gacacattct gggcagcgac
     8581 cacaggccat cacgaagggc acgacgaccc ggctgccgac caggccgggg tcggcgggcg
     8641 cttcgacgac ggtcccggtg aactcgtgac ccggcacgca cggcagcacg gtgtcgctgt
     8701 cgtgaccctg ccagccgtgc caatcgctgc ggcacaggcc ggttcgcgtc acgctgatga
     8761 gtgcgccgtc ctgctccggg gccggcaccg ggaggtcgac cacgtgcacc ggcccggcga
     8821 cggaggtgta ttgcagtgcc cgcatgcccg ctagtttcgc agccggaccg gctcggctac
     8881 gacgcccctg atccatcggt ggtcaggccg tcggcgacgt ggccgtgaga tctgcaggca
     8941 gcggcgtgct gtggatgaca tcgagttcgg agacggcgcg ggtcagcgcg acatacaggc
     9001 ggcgcaggcc cgtgcgctcg tcgggctcgg ccgcgacgat ctgcgccggt tcgaggacca
     9061 cgactctgtc gaactccaga cccttggcga ccgaggccgg gacgatgtcg atctggtgat
     9121 tgacatcgcc gtgatcgacg cccagtaccc cgtgctcgag tccggcccgc cccaacgccg
     9181 cagacgcctc ggcgacgcgg gcgtccggca cgatgagccc gaccgacccg ggcgcggcgg
     9241 tcaccgagat cgccagtgcc acaagccgat ccagctcttc gccggccgtc acggcgtgca
     9301 cgtcgaggcg tccgggattg tcacgaaccg actgcggcgc ccccagtccg ggggcgatcc
     9361 gcggcagcag gcgcgcggca tacctgatga ccgacgccgg cacccggaag ccccggtcga
     9421 ggacctcgat gtgggcgtcc gtcttgccga gatgcctcaa tgcctcgttc cacgacccgg
     9481 tggcccacgg cgtggtgccc tgggcgatgt ccccgagcac ggtcaccgaa ccggtggtgc
     9541 agcgccgccc gaccgcgcgc agctgcatcg gcgaaaggtc ttgtgcctca tcgacaatca
     9601 cgtgtccgag gctgggggtg cggtcgagca ggtctcgcaa ctcgtcgagc agcacgaggt
     9661 cggcgacgct ccacttcgcg ctcccggacg tctttgccgg gcgctcccag aggagcgccg
     9721 cctgttcatc gggcgagagc agatcgccgg ccgcggccgc gagcgtgtcg gagtcgctca
     9781 agagccggtg cagtagttta gccggatcga gcacgggcca gagggcggcg gcatacgtct
     9841 tgaccgcgct gctgcgcgcg accgcatcct gcacgcggtc gtcggggctg tcaccggccc
     9901 gctccatctg cacgagcacc gcgtgcgcca gacggtgcgg cagcatctcg cgggcggcgt
     9961 ggtagcgcgc accccgaccg cgcagctcgt cgacgagatc acccacctca tacgcgggca
    10021 cgcgccattt gcgcgaacca cgcggtgcca caaggggttc cgtcggtgcc acgacgtgcg
    10081 accagacgaa acggcgcagc atctcggcca tccggggatc gcccttgagg gtcgccacgg
    10141 ccggggtgtc ggtggccttg accgggcggg tggcgaacat gtccgcgacg gtcgtatgcc
    10201 cgacacgcac ctcgccgaga gccggcagca ccgcgccgat gtggtcgagg aaggcccggt
    10261 tgggtccgac gacgagcacc ccttggcggg aaagacggtc gcggtgggca tacagcagcc
    10321 aggccgcccg gtgcagcccg accgcggtct tgcccgtgcc cggcgctccc tggacgcaca
    10381 cgctggtgtc ggcaccggca cgcacgatga ggtcctgctc cggctggatg gtcgcgacga
    10441 tgtcgcgcat cgggccgacg cgtggccgct cgatctcggc ggcgaggatc gcgctcttgg
    10501 tctccgattc gcctgtgtcc gtgaggtgtt cgtcctcgta tgccgtgatc tcacccccgg
    10561 cgatgccgaa cctgcgacgc aggaccaccc ccatgggctc ggtgcgcgtc gcgcggtaga
    10621 aggcgccgga gacctcggcc cgccagtcga tgaccagcgg gtcgccgtgc tcgtcgccga
    10681 cgtgacggcg gccgacatac caccgctcac cgccgtcggt gtccagccgg ccgaagaaga
    10741 gggccgtgcc ggggtcgtcc cgcagcgcct gggcccggta gtacagggtc cgggcgaggt
    10801 atgccgcggc gatgggatcc gcggcggcga cgtcgagggc ctcggtgcgt tcgcgcatcc
    10861 gggtcaaggc ggtccgcgcc gccttgaggt gggcccgctc atgttctagc tcagtttcgg
    10921 gcacaaggtc accccacgaa gttgtccgct cgttcctcgc tccgatacgt ggcggggacc
    10981 caggcacaag ggtctacgtt gccacaccac ccggcgctcg cgaaagctca ttatcgtgcg
    11041 caaaagaggt gggggctggg catcccttcc gccaagagag atgcccagcc cccgagggag
    11101 ggaggaaacg tcttatgaag cgggcttcgc ggcgagggtg acggagatgt cttgcgcggc
    11161 gccgttgcgc aacaccttga gggtgacggt gttgccgacc ttctcggcgc ggatgtgggc
    11221 gatgagcgag tccatcgact ccacgccgac acccttgatg ccgatgatga gatcgccgtc
    11281 cttgagcccg gccgtggcgg ccggggtgcc gggggagacc tgggcgacca aggccccggc
    11341 gcgggtcgcc gtgccgtcct tgccttcgcc gtcacgcagg ctgacgccca aataggcatg
    11401 tgtcgcagag cccttctcga tgagctggtc ggcgattgcc ttggcctcgt tgaccgggat
    11461 ggcgaaaccg atcccgatgt tgccgctgtt ggagccggcc gtggcgatgg aggagttgat
    11521 gccgacgagt tggccgtcgg cgttgacgag ggctccgccg ctgttgccgg ggttgatcgc
    11581 ggcgttggtc tggatggcgt tggtgtagac cgggtcgctc tgctgcccga actgggtcga
    11641 ctggtcgccg gtcgtcacgg gccggttcaa ggcgctgacg atgccggtgg tgacggtgct
    11701 ggcgaggccc agcggattgc cgacggccat gaccgggtcg ccgacggcga gcgcactcga
    11761 gtcgccgagg gcgatcggcg tcagttccgt gggcgggttg gtgatcgtca cgaccgcgag
    11821 gtcggtggag gggtcggtgc cggccacggt cgcctcatag atccgtccgt ccgagagggt
    11881 gacctgcagg gtgccctgtt cggccccgtc gacgacgtgg ttgttggtca gcacgtgccc
    11941 ggcgtcgtcg atgacgaccc ccgagccctg tgcctcgccg ccttggacct gcagcgagat
    12001 cgcgaccacg ctcggggtga ccgccttggc ggtcgcggtc cagtccggcg cattggggtt
    12061 gacctgccgg atgctctggg tgcccgaccc ggaggcggag gccgaggagt tctgcgaggc
    12121 ggcggacgtg gtggcactcg aactgccacc atcgtccgcc gcccgcacgg cggcatacgt
    12181 cccgccactg gccagcgcgg ccgagaggac ggagacggcc accaggcccg gccaggtccg
    12241 cttgcggccg gcaggcgggg agggaggagc ctgcgtgaca gcgggagcgg gaccgggtgt
    12301 ggaccacgac gccggagccg gagccggctg ggaggagggg tacctgggct gctcggtgcg
    12361 ctcgccgaac cacagcggcg ctgtggcgtc ggggtcgggc gtcgacgtag cggcccaggg
    12421 gtccgacgaa ggcgaagggg aggtctccgt cggggttttc ggctcgctca ttggtggctc
    12481 cttcgtgcgt ggtgatcaca gtcaagtccg cgaagatcaa gcgatgctgg ggcggacctg
    12541 tgaatcagct ggatgtggga gccgcgggga gctcgacgac gaaggtcgcc ccgccgcccg
    12601 gggtattggc gatacccacc cggcccttgt gccggccgac gatcgcagcc acgatggcca
    12661 ggccgaggcc ggtgccgcca ttggcccggg accgggcctt gtcctcgcgg aagaatcgct
    12721 cgaaaacctt tgtgcgcgtt gcctcttcga caccgggccc gtggtcgcgc acctcgacga
    12781 cggcgcggtc gtcgcgccgt ccgacgacca cttcgacggg tgtcccgggc ggggtatgcc
    12841 ggatggcgtt ggtcagcagg ttgccgagga cttggtgcat cccggcttcc tctccgtgga
    12901 cgagcgtggg agacagggcg cccgcgagcg gctggacgac gatgcgtcgc tccgggtcac
    12961 gcacccgcgc cgccgcgacc gcctcggcgg ccacgacggt caggtccaag ggagcgaact
    13021 ccaccggccg ggcctcgtcg aggcgggcga gcagcaggag gtcgtcgacc aggttgctca
    13081 tccgggcggc ttcgctctcg atgcgggcca tcgtctccgg cacagcagtc gccggtacgg
    13141 ccccctgccg gtagagctcg gcatacccgc ggacggtcgc gagcggtgtg cgcaactcgt
    13201 gcgaggcgtc ggcaacgaag tcacgcatcc gctcctggga ggcagcgcgc acgtcgaagg
    13261 actcctcgac gtgggtgagc atggcgttga aagaccttga gagcgaagcg acttcatgcg
    13321 gcgcatcgct ctcgggcgcc cgctgcgaca ggtcgccggc cgcgatggca gcggccgtgt
    13381 cctcgatctt ggtcagcggg cggaaggcgc gggcaacggc gaaccaggcg agcacggccg
    13441 acacggcgat cgtcagcaat ccgatgagta cggccatctg gaccaggcgc catacggtct
    13501 tgtggatgtc cgcgagcggc tgcgcgacca ccgcgatggc gtcgccgccc tgcgacgcct
    13561 tggccacggc ccgccactgc agggtcgagc cgtccgtgcc gagcgtgaag ggggtgcccg
    13621 tgacgacgcg cgagtcctcc agcgacaggt cggagatcgc cgggtaggcg tcgacatcgg
    13681 tcgccgggat ggccgtgggc cggctgccgt ccgtcgggat gatcacgact gcgtatccgg
    13741 tcggcatccg gtcggtggtg ccctggagga catcgaccgc ccggttggcg atcgacgcgg
    13801 aactcttgcg gaggttgtcg tcgacgcggg agatgagctc gcgcttcatc aagaacgcgg
    13861 tcgccgccga cgtcagggtc agcgcagcga gcagcagagt gagcaggatg gcgatcagcc
    13921 gccaccgcaa cggaaggcgg tgcaggcggg cggcgagcgt ggtcacgcgg ggggcttgcg
    13981 caggacgtag ccgaccccgc gcttggtgtg gatcagcggc ggcagaccct cggtgtcgat
    14041 cttgcgccgc aagtaggaga tataggactc gacgatcccc gactcgcccc ggaagtcgta
    14101 gtcccacacg tgatcgagga tctgcgcctt ggacaacacc cggttggggt tgagcatcag
    14161 gtaacggagc agcttgaact ctgtggggga ggtctcgatg ggccgtccgg cgcgccgcac
    14221 gtcgtggctg tcctcgtcga gttcgagatc ctcgaagcgc agcatggccg agtcgtcgat
    14281 ctcgcccttg gtgcggcgca gcaccgcgcg gatgcgggcg atgacctcct cgaggctgaa
    14341 cggtttggtg acatagtcgt cgccgccgac ggtcaggccc ttgatcttgt cgtcgaccga
    14401 gtcacgggcc gtgacgaaga cgatgggtgc ccgcttgccg cgctcgcgca gcttgcgggt
    14461 gacctcgaag ccgtccatgt cgggcagcat cacgtcgagg acgacgagat cggggtcgtg
    14521 gtgggcggcc gccgtgatgg cctcgtgccc gtcccccgcg gtgacgacct cgaagccggc
    14581 gaactgcagt gaggtggcca gcagttcgcg gatgttcggc tcgtcctcga cgatcagcag
    14641 cctggcttcg ggttccgcga tggtcatacc tcccagcgtg ggcgccaacc ctgggagttt
    14701 cctgtatgcc gtccccactg tcgatggctg tcgaggcccg gtgggttcct agggcagcgg
    14761 ttgccagtcg agcgaggtga cccactgact gtcgtccggc acgtcgtaat agaagaggtt
    14821 gaggcagccg gtcgtcacgc agaagctctc gatctgggag ttttcccagc cgtacccgga
    14881 gatggcttgc ttgccgtcgg gtgagaagac gggatcgccg gaccaccctt ccgagcacgc
    14941 cgtcggcaat gatccgcagc gacccaccgg acaggttggt cgtcgccagc ccggcgtcat
    15001 cggggtgtcg tagtccgccc acgcccacag caccttcgac ccgtcggggc tgacatcggg
    15061 gcgcctcccg tcgtagacga ggtggtaacc gttcccggtg acgccggagg cagggatcac
    15121 ggtgtcgcgg acagggtgaa gatctgcttg ttgatctcga cggcgacccg gccgttgcgg
    15181 ccgggggagg cggcgtcggc aggtgcggtg accaagggca gggtcaaagc ggtggcgagg
    15241 gcggcgctcc cggtggcgag agtgcggtga cgtgcggtga acccgcctgg tcttcctccc
    15301 agatcgaacc ggctgtatgc cggggcctgc tgctaggagg ccgacgcggg gtttcgtgat
    15361 acctcgggac gccgactttt cgggagggtg cccgtcggca gctctcggac gtcagccgac
    15421 ggcggcggcg agcacgccga ccagggccgc ggcggcgatg aggaagagcg cgaccaaggc
    15481 cgcgagcccg agggcgaaag cccaccagcc gtggcgggcc agccgggccg aacgctgcgg
    15541 atcgctctgc gcggcgaggg cccgcacgcc gaagacgagg gcgggcacct gggtgagcat
    15601 gaacagcggg aagaggcaca acgacgacag cacgaccagc gcgatcgccg agccgttcac
    15661 ggtggggcga gcggcggcat accgtgaaga cccgtaggga tcaccggtgg tgaccggccc
    15721 ggcatacacc ggccggccag gtgcggagcg aacgtggtga cccggccgcc ggtaggcgtc
    15781 cggcgcgggg gcgtcctgga cgcgcgagcg gggcatgccg cggggcggga agggttgcag
    15841 cccagtgcgg ggagggggcg tgcggcgcat ggttccagcc tgtcgcgaga atggtgacgg
    15901 tgctatcggt gaaaccccga aacagccccg gatagttccg ggcgatgcgg ctgcgctgag
    15961 gtccgggggg atatcgacta gggcgcgggc gggagacgcg ttagccttgg ggccatcggg
    16021 tgcgctggcg cgtgcccgtt tcgaacgctt aatgagtaac gaggtgcacg gtgccgactg
    16081 gcaaggtcaa gtggtatgac gccgagaagg gcttcggctt catcgacgcc gacgagggcg
    16141 aggacgtctt cctccacgcc aacgcgctgc ccgcgggcac gaccacgatc aagaacggta
    16201 cgcggatcga gtacggcgtc gcccaggggc ggcgcggcgt gcaggcgatg ggcgtgcaca
    16261 tcctcgacgc gccgccgagc gtgcaggcca accgccgcga gcgtgaccgt aaacccaccg
    16321 aggacatggt cgtgatcgtc gaggacgtca tgcagttgct cgacaaggtg tccgaggggc
    16381 tgcgccgcgg acactatccc gacaagaagt tcgcgaccgt ggtgtcgcac gcgctgcgcg
    16441 gcgtcgcgga caacctggag tcgtgatggc ggcggccgtg aagtccgact cggccctgct
    16501 tgcggctgtc gacatcgcgc tggccgaact gaacgagagc gccgaaccgg gcaccgtggg
    16561 cgatcatctc ggcgccgtca tgagtggcga gcgcctggcg acccactcct tcgcctgcct
    16621 cgcgccggcc tatccgggct gggtctggac ggtgtcggtc gcccgcgctc cgcgcagcaa
    16681 gacggtcacg gtgtgtgagg tgaccttgct gccgggggcc gacgcgctcc tggctcccga
    16741 gtggttgccg tatgccgacc ggctggcccc gggcgatatc ggcccgggcg atgtcacccc
    16801 ctatgtcgcc gacgatccga tgctcgtgca gggcttcgag gcgaccggtg acgaggacgt
    16861 cgaccgcact gccttctggg aactcggtct cgggcgggcg cgcgtgctgt ccgccgaggg
    16921 gcgggaagcc gccgcggcgc gctggtatga gggggagaac gggcctggtg ccgcgctcgc
    16981 cctgaaggcg ccggctccct gtgcgacgtg cggcttcttc gtcccgatcg cgggggcgct
    17041 gcgctcgatg ttcggcatct gcgccggcga gtggtccccg tccgacggca ccgtggtcag
    17101 catcgatcac ggctgtggtg cccacagcga ggtcgacatg gaccggcccg acccggagaa
    17161 ggtgcccgcc ccgatcgtcg acgagtaccg cctcgacctt gcttgacggg ttctgctcgg
    17221 gggtcctcca ggccagcacc tcagctcagg tggcgccggc gtgttggtaa cagttctcac
    17281 tcaaaccagc tgagttcttg tgtgtgtgtg tgatccgtct agcctcatga gatggatcgt
    17341 cgaggcaaac gaaaggcact gggtctggtc tccgccgtgg tcctcactgc gtgggttcca
    17401 tatcgggctg ctgaggccga ggagctttcg gccccggtgg acaatctgtt ccgcacgtga
    17461 cgctggacat cccttgcttt gaccaagatc caggcggtga caatttcccg tgtcaaacag
    17521 acagtgccgg actggtctat tacattcaaa gtagcgttga cattggggac tcaacgcaac
    17581 tgggcacgcg gcgagttgtc aaaacgatga cggaacagtt cgacccgact caccttgttg
    17641 tcacccgcac gtcaaatcct acctacacag gtacaggaat tacggacatt atcttccaga
    17701 agagctcagt tggctttgag ggttctacca taggatttac ctggtgtgac gacgcgctgg
    17761 acggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaatgc tcacgctagg
    17881 cgggtgctcg ggccaggggg ccgtcactgc cgaggcccag gaatttttcc cgagctcaac
    17941 tgcagaggac tgggtcacct acagcgatgc gatcgtgaac attcacgtcg agcgggaatt
    18001 cgagattcct ccatcggcgg atgagctcga ggcggatgaa ggcattatcg ggcgcaatgt
    18061 ttcggctacg gtcaaggagg tcatgtggcg ccagccgtca cgcagcgatc tcgccatccc
    18121 gctgtctctc gatctgggag cgggcggctg ggtgtttcat ggctcggagc ggaaggagct
    18181 gcgcttctcc ggctcgccgc ggctggaacc ggggcacgac tatctgattg ccctggccta
    18241 caccacgctg catcccaccg gctccggcac gcccgcctgg atacccattg gcggccaagc
    18301 catcgtccct aatgattccg cgacgattgg caagggtgag gacatcgctc ggcccggcgc
    18361 gctcgccaag tcgctgcagg gccggcccac gtccgatctt gtcgataaga tctgggagac
    18421 agcgccggat ccaaaggcca agggcctgct tgacgagccg gcctccgtgc ggcgcactgc
    18481 ggtccaggca gcgagctgag catctcagct cagtattggg gctgggggtt ggccggctcc
    18541 tgcgggtgcg cgtcggcggc gttgccgcgg ccgcggcgga cgtataggta gccgatggcg
    18601 cccaggacga tcccggcgac gcacgcccag ggccaccacg agcggctgcc ttcgtgcaac
    18661 gtcggcacga cgagggtgac gatcaggccg acggcccaca ccgccatacc gatcaggacg
    18721 atccggtcca tccggaagcc gatcggctcc ggcgccctcg gggcaggtat gccgttgtcc
    18781 cggcgcagcg tgctcacggg ggatgagttt attctcgcgc cggtgtcgcg gggacattgg
    18841 cgttgcgtgg cctcacggcg cggtggtcct gggcactcgt ccagcgcaca tagggttggc
    18901 agtcatggcg aacagttcgc gggcaacgac ccgcattacc ccccaggaca acgccaccgg
    18961 cctcgatcgc ttcttccaga tcaccgcgcg cggctcgacg ctctcgcgcg agatccgcgg
    19021 tggcttggcg accttcttca cgatggccta catcgtcgtc ctcaaccccc tcatcatcgg
    19081 cacccaggaa gactcgacag gcgccttcct cggtggcggg tctgctccga acctcgccat
    19141 ggtcgcggcg acgaccgccc tcgtggcagg cgttatgacg atcctcatgg gggtcgtcgc
    19201 gaacttcccg ctggcgatgg caacgggctt ggggctcaac gctttcgtca cgttcggggt
    19261 ggcgaagctg ccggagatga cgtgggccga cgcgatgggt ctggtcgtcc tcgagggcat
    19321 catcatcacc gtcctcgtgc tcaccggctt ccggacggcc gtcttccacg cggtgccgcc
    19381 acagctgaag accgcgatct cggtgggcat cgggttgttc atcgcgatca tcgggttcgt
    19441 cgacgccggt ttcgtgcgca agagcgccgg cggccccctc ggtgaactcg gcgtcggcgg
    19501 attcctcgcc ggctggccgc tgctcgtgtt cgttctcggc ctcttcatca cggtcgcgct
    19561 gctggtgcgc aaggtgcgcg gcgccatcct ctacggcatc ctcgccgcca ccgcgctcgc
    19621 gatcgtcgtc gaggccgtcg gcaagatcgg cgcgcagacc aacgccgccg gcgagcgcgt
    19681 gaacccgacc ggctggggcc tgaacgtgcc gaagctgccg gactccgtcg cccagacgcc
    19741 ggacttctcc ctcctcggcg acttcaacct gctcggctcg ttccaggcga tcggcttcgt
    19801 ctccgccgcg ctgttgatct tcaccctgtt gctggccgac ttcttcgaca cgatgggcac
    19861 gatggtcgcc atcggggccg aagccggcct caacgacgaa gagggcgtcc cgccgcgctc
    19921 ggaggccatc ctgctcgtcg actcggtcgg cgcgatggcc ggcggcgcgg cctcggtctc
    19981 gtccaacacc tcatacatcg aatcggcctc cggtgtcggc gagggcgccc gcaccggtct
    20041 ggcgtcggtg gtcaccggcg tgatgttcct gctcgcgacc ttcctcgcac cgctggtctc
    20101 ggtggtgccg tatgaggccg ccacccccgc gctcgtcgtc gtcggcttcc tgatgatgca
    20161 gcaggtcaag gacatcgact gggacgacat cgagatcgcc ctgccggcct tcctgacgat
    20221 catcctgatg cccttcacct attcgatcgc cgtcggcatc ggggcgggtt tcgtgctcta
    20281 tgtgctgatc aagatcatca agggcaaggc cgggcaggtg catccgctga tgtggatcac
    20341 cgccgcgctc ttcctgctct acttcgccat cgacccggtc aagtcggtcc tcggcgtcag
    20401 ctgatcccta cttcgtcgcc cagcacgacg cctggcggtc atgaaccgct gggcgtcgtg
    20461 tcgtggttga gtgggaatac ttagctgaac taatgagtta ggctaagtat catgcctgct
    20521 gcctcgacga ctcagatcac cgatctggcg accacgctgc ggctggcctg catgcgcatc
    20581 tcgcgtcgcg tgcggttcga gagcaccgaa gccattgcgc cgcaccactt ctcggtgctc
    20641 atgcacctga gcaagggcga acgcatgccc ggtgaactcg ccgccctcga gaaggtgtcc
    20701 gcgccgagca tgacgcgcac gatcggtggt ctcgtcgatc tcgggtatgc cgcccgctcg
    20761 gtcgacgccg acgacaagcg gtgcgtccgg gtcgccatca ccgacgcggg ccgcgctgtc
    20821 ttgaaggaca cgctgcgttc gcgcaacgcg tggatggcca cccgcgtcgc cggcctcacc
    20881 ccgcgtgagc gcgaggtgct ggccgaggcc agcatcatcc tcgaacggct ggccgcggaa
    20941 tgagtcccac cttcgcctcg ttgtcggtgc gcaactaccg catctggttc ttcggcgcgc
    21001 tggtctccaa tgtgggcacg tggatggcgc gcgtcgcgca ggactggctc gtcctgacgg
    21061 agctgaccga gcactccgcg agtgctctcg gcatcgtcac cgggctgcag ttcctgccct
    21121 tcctgctgct cgcgccgtgg gccggtgccg tcgtcgacca cttcccgaag cgcacgctgc
    21181 tcatgatcac ccagatcagc ctgggcctgg tctccctcgt caccggcctg ctcgccgtga
    21241 ccggcgtggc ccagctctgg cacgtctaca tcctcgcgct catgcaaggc gtggcaacgg
    21301 ctttcgacaa tccggcccgg caggcattcg tctccgagat ggtgcccaac gagcgcctgg
    21361 ccaacgccgt ctcgctcaac tccgcctcgt tcaacgccgg ccgcctcatc ggtcccgccg
    21421 tggcgggcgt gatcatcgcc gcctggggca ccggcatcgc cctgctcctc aatgccgtca
    21481 ccttcggcgc cgtgctcctg gcgctgacat cgctcgatcc gcgcgccctc accccggcgc
    21541 cgctgtcgaa agggcgcgga tccatccgcg aaggcttcga ctacgtccgc ggccgccccg
    21601 acctgaagct ggtcatgggg ctggtgttcg tgctcggaac gttcgggatg aacttccaga
    21661 tcacgatggc gctgatggcg accaaggtct tcggcaaggg gccgaccgag ttcgggctgc
    21721 tcggctccat catggccgtc ggctctctcg ctgccgcgct gtggtccgcg cgccgggcca
    21781 acccacgcct gcgtcacctc ctcatcgccc tggtggcctt cacggtgagc gcgaccgcgg
    21841 cggcgctggc gccgacatac agcctgttcg ccctcacgct gatccccctc ggtctcgcag
    21901 cgctgagcgc gctcacgacg gccaatgcga tggtgcaact tcgcacgacg cccgccatgc
    21961 gcgggcgggt gatggcgctc tatatggcga tcttcatggg cggcacgccg ttcggtagcc
    22021 cgctgatcgg ctggatcggc gaccacttcg gtgcccggtg gacgatcggg atcggccccc
    22081 tcgcgatcgg cttgacgctg gccgcggtgg cggtctatct gtggcggcac gagaatgtcc
    22141 gggtgagctt cactcgcggc gcccggccgc acctgcgcat cctcagcggc aatgacgtca
    22201 tcgtcccgga gccggcgcga tgaccccgcg cttccgccgc tatgccacca tcggcgtcct
    22261 ggtgatcatg ctcgcctggg tcgtcctcgc cgctctcctg taaccggcaa aggcgccgcg
    22321 gactacccgc cggtaggtaa gacggttggg cggttataac cgccgaggtg tcttacctac
    22381 tcgcgggtca tgagcggcta catccgctcg atgacgtagt cgatggcgcc ggtcaggcgg
    22441 gtgatgtcgt cggggtcgac agcggggaac atcgcgatcc gcagctggtt gcggccgagt
    22501 ttgcggtagg gctcggtgtc gacgattccg ttggcgcgca acaccttcgc gatcgctgag
    22561 gcgtcgatct cgtcggcgaa atcgatggtg ccgacgacct gcgagcgggc agcggggtcg
    22621 gcgacgaagg gggtggcgac gtccgactgc tcggcccagg agtagaggcg cgaggaggag
    22681 tcggcagtgc ggccggtcgt gaactccatc ccgccgttgc cgttcatcca ctccagctga
    22741 ttggccatca tcgccagcgt cgcgacggcg ggagtgttgt aggtctggtc cttgcgcgaa
    22801 ttgtcgatcg cggtcggcag cgaatagaag tccggcaccc accggccgct cgccgtgatc
    22861 tcctcgaccc gggccagcgc ggcgggggag aagagcgcca gccacaggcc gccgtcggag
    22921 gcgaaacact tctgcggggc gaagtagtag acatcgctct gcgcgatatc gacgggcagg
    22981 ccgcccgcgc ccgaggtggc gtcgatcagc acgagcgcgc cctcgtcggc accctcgacc
    23041 cgctgcaccg gcgccatcac cccggtcgag gtctcgttgt gcggccacgc atagacatcg
    23101 atgccggcct cggccgaagg cgttgcgaga gaacccggct cggccttgat gatggtcgac
    23161 tcgccgagga acggggcctt cgaggtgact gagccgaact tcgcggagaa ctcgccgaag
    23221 gcgaggtgct gggcacgctc gcgaaccagg ccgaatgcgg cgatatccca gaacgcggtc
    23281 gatccgccgt tgccgaggac gacctcatac ccctcgggga gctcgaagag tgtggtcagg
    23341 ccctcgcgga cggccttgac gaggttcttg accggagcct gccggtgcga ggtcccgagg
    23401 accgaggcgc cgatggcgga cagggccgcc acctgctcgg ggcggacctt ggacgggccg
    23461 cagccgaatc ggccgtcggc ggggagcaga tcatcgggca ggcgcagatc ggtgagggca
    23521 gtcgtggtca cgaactccat acaagcaaag acaccccgcg cgaacgcggg gtgtccagca
    23581 tcggggcgat cacacgccgg cgatcacact ctcggatcgg gcaccagggc gtggatgggc
    23641 agatcgttgt ggtcgcggcc gacatagccc ttgaccgacg acaacgggcg gggaccctcg
    23701 ccgacatact tcgcgctcgg ccggatcagg cgaccggtct tcttctgctc gaggatgtgc
    23761 gcggcccagc cggcggtgcg cgcggagacg aacagggggg tcatcatctc gccgggcacg
    23821 tcggcgaagt cgagcagcac ggccgcccaa tagtcgacat tggtctccat cttgcgctcg
    23881 gggtagcgct cggcgagcgc cgcgatggcg gcccgctcga gttccagagc cacctcatac
    23941 cgcggggcgc ccaggcgctt gcaggtgtcg cggagcacct cggcgcgcgg gtcgtaggcc
    24001 cggtagaccc ggtgcccgaa gcccatcagc cgctcgcccc gcgcgagggt gtcggcgacg
    24061 tacttgtcgg catcgcccat ctgctccacg ccctcgatca tgtgctgggc gcgcgaggga
    24121 gccccgccgt gcagcgggcc ggacagggcg ccgatggctc cggagaggca ggcggccacg
    24181 tcagcgccgg tggaggcgat gacgcgggcg gtgaaggtcg aggcgttcat gccgtgttcg
    24241 gccgccgaga tgaagtaggc gtcgatggcc tcgacatgcg ccgggtccgg ctcgccgcgc
    24301 cagcggatca tgaagcgctc gacgatcgtc ttgccctcgt cgacccggct ctgcggaacc
    24361 atcggcgtgg cctggccgtg cgcggactgg gcgatgaagg acaaggtcat caccgaggcg
    24421 cgggcgaggt tttcgcgggc ctcctccgga gagatgtcca gcagcggctt gaaaccccac
    24481 accggggcca gctgcgcgag cgcggactgg gcgtcgacgc ggatgtcgcc ggtgtgaacg
    24541 ggcagcggga acggctctgc gggcggcagg cccgggtcca tctcgttgtc gacgagcagg
    24601 ccccatacct ggccgaagct gacgcgaccg acgagttcct tgaggtcgac gccgcgatag
    24661 cgcagcgcgc cgccttcctt gtcgggttct gcgatcgacg attcgaatgc gacgacccct
    24721 tcgaggccgt gcttgacttc gctgtcactc attggaggat tgccccttcg cctgatccgt
    24781 ttcgttcaag cgatcacctt aaggcaggga gcggacaact ccgacacctc cgctgtcggt
    24841 gggcgggcat aacctgcccg catgaccacc ccactcgctc cgcgccgcga ctatcccggc
    24901 gatggcctgg ccgaggacca gctcgcgccc acgccctatc gacaggccca gctgtggatc
    24961 gaggaggccg tcgcccgggc cgagacgggt gacgacgtcc ccgagccgac gtcgatggcc
    25021 gtcgcgaccg tcgacccgtc gggccggccc gacgtgcggg tggtcttgat gcgcttcttc
    25081 gcgcccgagg gtcccggttt cgtcaccaac ctgaactcgg cgaaggggca gcagctggcc
    25141 gccaacgacg gggtcgctgc cagtctgacc tggcctgcga tgttccgggc catccgcttc
    25201 cggggccggg ccgaggtgct gcccgacgaa ctgatccgtg actatttcgc ctcgcgaccg
    25261 tgggggtcgc gcatctccgc gttggcgtca gagcagtcgg cgcccatcgc ctctcgcgcc
    25321 gagctcgaac agcggtatgc cgcgctcgcc gcgcagtatc ccgacaccgg ttcccccggc
    25381 gatgtgcccg tgccggacgg atggggtgga tatcggatcg tgcctgacga ggtcgagttc
    25441 tgggccggcc ggccgtcgcg gctgcacgac cggatcgtct tctcccgcac cggatccgga
    25501 tcgatggacc acgccgccgc gtggtcggtg agccgccgcc agccgtgacg acgcttaccg
    25561 gcccggcgca gccgcaggac gggccaccct tccggctgcg gtcggcattc ctcttcagcg
    25621 ccgcgatgtc gccgctcgcc ttcgctctgg tgctgcgggt gcccgacctg ggcgccgacg
    25681 cgcgggatga ggcgcgcgag gaccccgcct cggtgctcga cgtgctcggc acgcacccgg
    25741 cgtacgctcc tcacgctggg cctgggcgcg agcgcgatct cggcgctgcg cggcatacgg
    25801 ctgagcgtgc tgcccccgtg ggccgaccac atcggcctgc cggcggcgca gctctcgctg
    25861 ctcgtcgccc tcggggcgct cgtcgagatc gcgatctgct atccggccgg gtgggtgatg
    25921 gatcggcgcg ggcgggtgtg ggtcgccgtc ccgacggcgg tgctgctggg gctcgggctg
    25981 atgctgatcc ccgtgaccgg gtcgttcggc gagttgctcg ccgcgaccgt cgtcatggcg
    26041 gtcggcaacg gcctcggctc gggcatcgtc atgacactcg gcgccgacac cgcaccgcgg
    26101 ctgcaccgag cgaagtatct cggtggctgg cgcctgttcg ctgacatcgg catgtccggc
    26161 ggcccgttga cggtcagtgg tctggccgcg ctggccgggc tcgcgagcgt gatcctgggc
    26221 gtgggtgggg tgcttgcggc cgggtggccg tggcgctggg tcggtcccgt ggaccggcgg
    26281 cgccgggcag atgagaggat gcccgcgtga gtgacctgat cgacaccacg gagatgtacc
    26341 tcaagacgat cttcgaactg gaagaagagg gcatcccgcc gctgcgtgcc cgcatcgccg
    26401 agcgcctcgg ccactccggg ccgaccgtgt cgcagacgat cgcccggatg gaacgcgacg
    26461 gcctggtgtc cctcgccggt gaccggcacc tggagttcac ggcgagcggg cgcgagcggg
    26521 ccacccgcgt catgcgcaag caccggctcg ccgagcggtt gctggtggac gtcatcggcc
    26581 tggagtggga gtacgtccac gacgaggcct gccgctggga gcacgtgatg tccgagcgcg
    26641 tcgagcggcg gatcgtcgag ttgctgcgcg gcgatcacac ctcacctttc ggcaatccga
    26701 tcccggggct ggacgagctc ggcgcgacct cggtgcggga gggctttcgc gacgggctgc
    26761 agacgctgac ctccgtcgcc ggtcccaacc cgacccgggt gaccgtccgg cggatcggcg
    26821 agccggtcca ggtcgatcac gaggcgctca acctgctcac cgattccggg gtcatccccg
    26881 gggaggccgc cgaggtgcat gccgcggacg gccgcgtgat cgcagtgggg gccgggcgac
    26941 cgcctgccga cggggtctcg ctaccgcccg agatctccga ccacgtcttc gtcgcgctca
    27001 cctgacacca caccgcccgc tcgtctcacg aatggcgccg cgccgtgacc tgaatgtgac
    27061 attttcgtgt cggatgtgta cggtcggtcc agtgcctctc aggcgggagg cgccagtcgc
    27121 ccggatcgcc gagtcctacc cccggatctg actgaacact cgaacaccgc ttggtaggag
    27181 cgggggaccc acttgcgggc accgactccg gtcggggtcc ttggggtgaa gccggcggat
    27241 ccgtgtgatc accggccggg cagcaatctc tcgcccgaac ccgacagctc acctcgtagg
    27301 cgtcgagagg tcacctgtgt cccagcacgc tcccgggcgc caccgcgctc gccgtcgtcg
    27361 tcgcgacggc ctccgtcgca ccagcgccgt cctcgctgcg accggcggca tgatcaccgc
    27421 cttcgccgtc tccgccgacg cggtccccgc tcccgctgtc accacccccg tgcacaaggc
    27481 gccggccgtc aaggcggccg ctcccgccct gaccgcgccc agcgccgccg cgcccgtgaa
    27541 gaccagctac ggcgttctcg gcgtcaaggc ggtcgccaag ccgaagccca agccgaagcc
    27601 cgccccggtg gtgaccccgg tcgcgaccac gacgtcggcc accgccgtgg tcgccgcgcg
    27661 cacccagacc gcccccgcga gccggtccac cacgcgcacc tcgaccccgt cgaacagctc
    27721 gagcaattcg gcgtcgagca ccccgagcac cccgaccacc ccgcaggtgc cggccaacgg
    27781 cggtgtcctc gcgatcgccg cgtcgctgtc cggcattccc tacgtctacg gcggatccac
    27841 cccggccggc ttcgactgct ccgggttcac cagttacatc ttcgccaagg tcggcatctc
    27901 cctgccccgc acggccgcgg gccagcaggc cgcggccacc ccggtgagca acccgcagcc
    27961 cggtgacctg gtcttcttcg gctacccggc ctaccacgtg ggcatctatg ccggcaacgg
    28021 aatgatgtac gacagcccgc gcaccggctt gtcctcgggc ctgcgcgcaa tctggtcctc
    28081 ctcggccacc tacggccgcc cctaagactc agtcgtcccg cagcgggcga gaggtgcgga
    28141 tcttgcacct ctcgcccgct tcgtcgtgct ccgggcagga tgggcgccgg gggtggtggc
    28201 tcacaggttg gcttggggcg atcgactgcc tcggcgcact gctgatggaa ggaccggcga
    28261 tgtccacatc ccacgagacc ccatcccggg cggcataccc gctgcccacc agccgccggg
    28321 aggagtccga tctgccggtg acccagcccg tcgcgctccg gccggcggcc tgggtccagg
    28381 acgccgcgcc gccgcccgaa cgctcgcagc gccgacggta tgccgcccgg ctcagtcggg
    28441 catttccatc cgcgcgtcga tccaggtctc cttcgcgtca tggctgaaga ggacgaagcg
    28501 gatcttctcg atgccgccgc gcttgtgctt ctccatatcg gcctccgcca tcgcaatgcg
    28561 ggccacctcg aggtggtccc acccgtagac cccggcgccg atggccggga tggcgatgct
    28621 gtgcgaacgc agcccgcgcg cgaggctgag gctgttggcg aagcagcggc gcaggacctc
    28681 cggatccgtt tctcccgcat ggcgattcgg acccacggtg tggacgatcc acttgacctt
    28741 cttgaggttg taggcccggg tggcgatggc gtccccgggc ggcagaccgt ctgggagggt
    28801 gctggagcgc aggtccttgc actcctccag cagccctggc ccggcggccc ggtggatggc
    28861 gccgtcgacg ccgccgccgc cgagcagcga ggagttggcg gcgttgacga tgacatcgac
    28921 cttttccttg gtgatatcgc cctcgacgat ctcgatccgt cccatggccc cacggtatgc
    28981 cgcgtggcag gcttggtggc gacagcgctc ccggccagct gcacggcggg gtgagcgggc
    29041 ggcatacgac ggaagggcac caacggtggc ggacgagatc tactacgaca agaaggacca
    29101 gctgcagatc gtccagaact cactgctgcc cgacgagacc gtcttcgccg tctacgacgg
    29161 caaagaggtc ggcaccgggt tcatcgggct gaccgaccgg cgagtcatct tgcaggacaa
    29221 cagcttcgtc ggcgggagag tggcggtgac gtccgtgccc tacaacaaga tcacggccgt
    29281 gtcgtatgcg tccaatgcgt ccgtcttcgg ccggttcacc gaggagacga cggtcgcgat
    29341 ccacacctca cacgggacct acgaggtgct gttgcgtggc tcggagaagg ccaagcacac
    29401 ccacgacgtg atcctgcact acgtcgtgca ttcctagcgt tccggtcagt agtaccaggg
    29461 gtagggcgac cagtcggggt cgcgcttctc gaggaactgg tcgcggccct cctgggcttc
    29521 gtcggtcata taggccaggc gcgtggtctc gccggcgaag agctgctggc cgacgaggcc
    29581 gtcgtcgatg aggttgaaag agtacttcag catccgctgc gccgtgggcg acttgccgtt
    29641 gatcagccgc ccccactcca gcccggtggc ctccagatcc gcatgcggca cagcacgatt
    29701 caccgtcccc atgcgcacgc cgtcctcggc ggaatactcc tggccgagga agaagatctc
    29761 gcgggcgaac ttctggccga cctgccgggc gagataggcc gaaccgaaac cgccgtcgaa
    29821 ggagccgacg tccgcgtcgg tctgcttgaa gcggccgtgc tcgatgctgg cgagggtgag
    29881 gtcggagacg acgtgcagcg agtggccacc gccggccgcc cagccgttga cgagggagat
    29941 gacgaccttg ggcatgaagc ggatcagccg ctggcactcg aggatgtgca ggcggcccag
    30001 gcgcgcctgg tcgatgggat tgggggcggc ctggccggtc gcggtcgact cgtgctcggt
    30061 ctcgtactgg tagcccgcgc gcccgcggat gcgttggtcg ccgccggtgc agaaggccca
    30121 cttgccgttc ttctcgctcg gcccgttgcc ggtgagcagg acgcagccga cgtcggcgga
    30181 ggtgcgggcg tgctccagga cccgcagcag ttcgtcgacc gtatgcgggc ggaaggcgtt
    30241 gaggacatcg gggcggtcga acgcgatccg gacggtgccg tgctccttgg ctcggtgata
    30301 ggtcaggtcg gtcaggtcct cgaagccggg tatgccgtcc caccggtcgg catcgaagat
    30361 ctggctgact ccctcgatcg cactcatgac gggcaggcta tccgggcggg atggtcagct
    30421 tgaatccaag gtgggtgggc tcgaatccga ggcgctcgta gaagcggtgg gcctcgatcg
    30481 ccggtcaacg cctcggcgag cactacggcc ggcacgctcg gcggccacct gccgcgttca
    30541 ccctggccat gctcataaag tacgcatgac gccatcattg ccagcaacag caggcccgcc
    30601 actctcgccg catacagccg gaatacgagg agcgatgctc ccgcccgtcc gccacgctca
    30661 gcgcggcccc gggttcctcg tattccgaat gtccggcgta gcctgctgcg catgcggatt
    30721 gcgtccggaa ccgtgatcgt cctgctcgtg gccctgatcc tgctggggct cggcacggcg
    30781 atcgtgatcg cctcgtggga cgacaagccg gtggcgatct cggtgctggc catcatgggg
    30841 atcgggacga tggcaatggt caacggggcg ctgcaggccc gccgctacga ccgggacaat
    30901 ccgcgccgct gaacgacgac agcgggcccc ccgagaggtc cagctgtcgc gccgagtcgg
    30961 atgccggtca gcgccgggtg tgggcgaagc gctcctcttc ctcgtcctgg atggactcgg
    31021 gggagagggt ggccatcgag tcgaggagtt cgtgctgcgc ctcctcgttg ccggacgggt
    31081 ggttggtggt gcgcagcgag accgacttga tcagcgcggt cgcgacgaac accgcggcga
    31141 ggatgggcag cccgaggacg aagacctcgt gcaagcgctc ggcgagcccc atccgcacgg
    31201 cctcggccac gggtgcgggc agctgggcca tgacgctcgg atcgagaacc gatccggctc
    31261 cgatctcctg accagaggcc tcggcggcac gcgccgcggc gggggacagg tggcgcagga
    31321 tcgcgtcgtg caggcccgtg gtcatcacgg tgccgaagat cgcgatgccg accgtggacc
    31381 cgacattgcg gaagaactgg gtggaggcgg tggcgacgcc catgtccgtg aagtccgctg
    31441 cgttctggac gagcagggtg tattgctgca tgcacagacc caggccgacg cccagcacaa
    31501 ccatcgccag cgtcagctga cctgacgtgg cctcgtgggt caggcgcgtg agcagccaga
    31561 ctcctgcggc gagcgtggcg acgccggcca gcatgacggc cttgtaacgg ccggtcttgg
    31621 tgatgatctg gcctgcgacg atgccgaaga tgatcaggcc cgccatcagc ggcagcagga
    31681 tcaggccgga gttggtggcg ttgacgccga tgacgccttg ggcgaacacg gggatgtaga
    31741 tgatcgcgcc gaacatgacc atggcgacgc cgaaggcggc gacattggac agtgtgaaga
    31801 tcgagttctt gaagaggcgg agcggcagga tcggttctgc ggcacgggat tcggcccaca
    31861 cgaacgcagc gagcagcacg gcgcccacgg catacagtcc gaggatgacg gcggaggacc
    31921 aggcgtaggt cgtgccgccc cagctggtgg cgagcaggat cgcgacgagc gcgggagcca
    31981 gcgtggcgat gccgagcgcg tcgatcttgg cgttccggcg ctggtgcggc aggtgcagga
    32041 agcgcgccac gacggcggtg gcggcgatgc cgacggggat ggaggaccag aaaagccagc
    32101 gccacccgag gttgtcggtg atccagccac ccgccagcgg gccggcgacg gaggtgacgc
    32161 cgaagacggc gcccatgagg ccctgatact tgccgcggaa gcgcggaggg atgatgtcgc
    32221 cgatgatggt ctgcgccaag ggcatcacgg tgcccatgcc gaggccttgg atggcgcggc
    32281 cggcgacgag ggtccagaag ttcggggcga gaccgctgac gagcgagccg agcatgaaga
    32341 cgatcaggcc ggcgatatag aagccgcggc ggccgtagag gtcggagaac ttgccgacca
    32401 ccggcacgac gatcgcggag acgagcatgg cggcggtcgc gacccaggag tagtgctcca
    32461 tgccgccgag ttcggcgacg atcaacggca tcgccgggcc gacgatggtc tggctgatcg
    32521 aggcgacgaa catgccgagc atgagcccga tgaagatgcg ccggccggtg gcgtcgagat
    32581 ggaactcggt ctcggccggg ttggaagtcg tggtcacggg tcaagtgtag gcgaaagttg
    32641 cagagtctgc aaagttgcag ttactgcagt atgatgggtg catgacttcc gtggcggacc
    32701 acccggtcct cgtcgacgag gacgccaact gcggtctgcg cgaacgcaag aagcgcgaga
    32761 cccggcgggc catccaccgc gccgcactgg aactggcctc ggacagcggt atggacgcgc
    32821 tgacggtcga tgccatcgcc gagcgcgcgg gggtctcgcc gcgcaccttc ttcaactact
    32881 tcccggccaa ggacgacgcc ctcgtcgggg ccgaccccga cgacgacgtg gaccggctgc
    32941 gccgcttcgt ggcgtcccgc ccggacaccg agtcgccgcg ggaggtggtg catgcgctgg
    33001 cgctcgaccg cgtcgccggc ctcgaactcg atcgggagct gtgggacatg cgccgggagc
    33061 tgacccagcg cgagccggcc atcgggctgc gcgtcctggg cgtctacgcc cgcgccgacc
    33121 gcgccgtcgt cgaggccctg accgagcggg cccgagccgc gcgtgcgttg tccctcgatg
    33181 acgagctcgc tgtcaccgtg gagacgtatg ccgctctggg cgccttccgc gccgccgtcc
    33241 gcgcccacct ctccggctcg catgccgcga ccttcgccga actcgtcgac cgcgccttcg
    33301 ccatcctctg acgccccctt caccccgtcg aaagtgcgcg acccgccgca atgcgtgagc
    33361 gatgacggcg tgtcgcgcac tttcgaccgg ggtctggggc tccaactaga ctgtcccggc
    33421 cggcataccc cggcccccgc cctcgtagct caggggatag agcaccgctc tcctaaagcg
    33481 ggtgtcggac gttcgaatcg tctcgggggc actcaggacg tggcggcgag agccgtcagg
    33541 gcggcggtga agcactcctt cggcgggttg accaggccgg cgcccacctg gccgacgccg
    33601 gcgacgcggc cggccatacc cgtgttgatc tgcgggagga tgccggtgcg cacgaccttg
    33661 gtgacgtcga ttcccgttgg ggtgcctcgg aattcgagga tcgggatctg gtatgccggg
    33721 tgctcgccct gagtgatggc gtacatccgc tgggtcgcct ggagagcgaa cggcacgtcg
    33781 ccaccgacga acttcacgat cgccggggcc gccgccatcg cgacgccgcc gatgccgccg
    33841 gtctcggtga tcgccgagtc accgatgtcg gggttggcat cctccgggcc gtacgagccg
    33901 aggaagagcc cctcgggcgt gttcgccggg ccggtgaacc actgctcgcc ggtcccggag
    33961 acgcggatgc cgaagtcggt gccgttgcgc gccatcgtcg tcacgatgga ggagccgggt
    34021 atgccgtggg ccgcgagggt cgcgagcttg caggcgggca ttccgaggtt gaggaagaag
    34081 tgctcgttgg cacccgagaa gcgcaccgcc tcagcgatat ccgacggtga gccgtccgcg
    34141 gtgatgaggt tcggcagcaa ctcccgcagc agcatcaggg acccggcgcg gttgcggttg
    34201 tggccctcgt cgcccatctg cagcatctgt gcgacgatcg ctttgatgtc gatccgctcg
    34261 gccttgcgga tggcctgctg cagcagcggc ccgaggaccg agttcatcca ctgcagtcgc
    34321 tcgatgacct cgggaccgta ggcgccgtag cgcagcacct tgcccaggcc ctcgttgagc
    34381 gagcaccacg agcggttgtc gtggaccggg tcgtgcagtt cgtacatcca catgctcggg
    34441 ctgacgacac cggccatcgg acccaccgca tcgcggtggt ggcacggttc gagtgtgatg
    34501 ccctctcccg cagcgagttt cgcctcggcc tcctcgggag tgtcggccat gccctcgaag
    34561 agcatcgccc cgatcagcgc acccttcatc ggcccgctcg cgcgctccca atccagcggc
    34621 ggccccgcgt ggaggaaggt gcccttctcc agcccgaccg cctccgacgc cggtcgcaca
    34681 tcgacgaagt ccgcgccggc ggcggtcatc cggtcgaagg caagcttgtt ggcggcctcg
    34741 cggcgctggt cgagcaacac cgagcgcagg gccgccgtgt ccgagccggc gagcggcggc
    34801 tgccagcgcg cctccgtcac cgtcaccgcc tggtcggcca gcgcctcgct gaacaggccg
    34861 acgccgaccg tcgcgaccac gggttcgcgc ggcagagcca cagccgagcc ggcgcccggc
    34921 cgctcgccgg tccccgcggc agttgctgcc tcgacggggt cgaattgact tctcgacggg
    34981 atcgaatcga catctcgacg gggggtggag gtgccggtca ggtaggcaac ggcggcgcgg
    35041 gtcgcctccg cgttggagag gtatgccgtg gcgccggctt ccgcgagcgc gctcgcgcag
    35101 cgctccaagc cctgcgggtc gccttgggtg ccaacgagac tcacgatgac cggcagctcg
    35161 cgcccaccgt cggcggctgt cgcccgggcc gcgcggatcg cctcggccaa ctcgccggcc
    35221 gggtccgggt gggcgccata gccgagcacg aggtcgagca ggagcacgcc gcacgtcggg
    35281 tcggatccct cggccagaat gcgctcgtgg cgcagcgccg gatcgatcat cggatgggcg
    35341 cgcccctgtg tcaggccgtc gtcgccgaaa tcgatgacga cgtggccgcc gtcggccgcc
    35401 atgtcggagc ccaacgcgag ttcgggcgag agggggatat tggagcggat gggaccgagg
    35461 gcctcgccgg cgatcagcat cgcctcatcg gcgagcgtgc ctccgcagaa gaggccgcgc
    35521 agcgaacccc cacccgaaac cccgcccggc acaacgcctt ccaccgatcc ccacgtcggc
    35581 caagccggca cctccacacc cgaggcggac agggccgatt ccaccgatgc cgtcaggtcg
    35641 ggccgccctg cgccgaggat cgcccagcgc accgggtggc cgagaccggc ggcatacgac
    35701 tcgagatcgg cgagcacctc ctcggccggc ggcttcgaga cgacgaggat cgattcggtg
    35761 tcctcgtcgg cggcgagcat cgcgagggcc tgcttcgtcg accggccggc gacctccgac
    35821 gtcagatccc gcccaccgac accgagcaca tgcgagacgc cgaccccggc ggcgtcgagc
    35881 aggcagatca cttgttgcgc accggttccg gaggccgcga cgatgccgac cggtccagga
    35941 gcgaccacgt tcgcgaagcc gagcgccacc ccactgacga ccgcggtccc gcagtcggga
    36001 cccatgacca gaacacctcg cgccgctgcc gcgtccttga gcgcgacctc gtcctcgacg
    36061 gcgacgttgt cgctgaagat catgacgctg accccggcct cgatcgcatc gagggcctcg
    36121 gcgctcgcgt gctcaccggg cacggagatc aaggcgaggt cggcatcacc cgcggcgatc
    36181 gcgccgccga gggtcacggg ttcgggcgcc gcgccgaagc cggtgcccgc cgagcgggac
    36241 gctgccgtca actcggccaa cgccgaatcc accgctgcca caccggaatc cagtgcctcc
    36301 tgcgactcgc cgtggagcgc cacgacgacg tcgttgacgc cgatcccgtc gggcaacgtg
    36361 aaccccatcc cggcgagcac ctcgaggttg agttcggtcc ccatcgccac ctgcgccgcc
    36421 gtgatcccgg gcgtggtcgc gatgcgcttg gagacctgca tcagggtcac ggagtcgtaa
    36481 taggcaccag tgcggacgtc gaccaattca ggcacgggcg ggctcctttg tcagagaagg
    36541 ggattggtgg atctgccggt gggcgatagc gatttcgacg ccccggatca gggccgggcc
    36601 ggacgtgtgg ccgatgtctt ccaccgcgtg ccgggccgcc ccggcggcag cgcgattccc
    36661 cgacaggcgg gcatcggcat acgcaacgag gccgggcacg gcatatccct gcgccgcggc
    36721 gcgcaccaat gacgccgaca gcgccgtcgt gcgatcgagc tgggggagaa cggcatccgc
    36781 gatggacgtg tcgtggccgg cgaacgcgac cagcagcgcc ccgcagatct cgtcgtcgcc
    36841 ctcgggggtc aacccccggc cgccgccgag gcggtcggcg gcccacgagg ggggtagttc
    36901 gacggcatac gacccgctgc gacgcacccg ggccggccgc cacgtgcgga cggcgcgcac
    36961 cggcccagcc ggcagcagta tgcagtggtc caggctctcg atcacgtcac cggccgcgac
    37021 gccccaccgc atccgggtgg aggagtccgg cagtcgccat gccgtcggga gggccagcgc
    37081 ttccgcggtc acgaggggga ggaggccccg ctcggggtga tggagataga gcgcggtggg
    37141 gaaggccgcc agcacgtgcc agaccaactg ctccgcgccg accggaggcg cgagcggaga
    37201 gcgggcggcg aacggcatgt ggtcgaacct agcgaccccg gctagtctcc gcgtgtggag
    37261 aaagttgcca acgattccgg gctcggggat ctcgacttgc cagggcgcgc acgcacgcaa
    37321 agtgacttct ccctgtcact ggacgtcccg gacgacctca acaagcgcgg cctgcgtgag
    37381 cttgccgacc agctcgtcgc cgccctgcgc cgcgaggacg aggtgcatcg ctccctcatc
    37441 agcaccgtgg tcgccggggg ctccctggcc gacctgtgcc aagccctggc cgggatgatg
    37501 gacgccgtcg tcatggtgac caccaccgac gggcgggtga tcgcggaggc cggcgaggcg
    37561 gaggcggtgg ccgcggcata ccgctgcccg gcgttcgacc cgaccggccg cttcctcgtc
    37621 gaacgcgaaa gcgtggggga gcgcaccggc acggcggact gggtgcggcg ggcgtcggtg
    37681 cgcatcgtcg gcggctccgg ggcggagggt cgcatcgtcg ccatcgccga cgaccgcccg
    37741 atgacgggca acgacattca tctgctcgaa cgctcggcca cggtcgccgc gctcgccctc
    37801 gcccgggagc aggcggtgct ggcggtggag agcaagtacc gcgccgagtt cctccgcgac
    37861 gcgctgtccg gccgggccgg ctccagcgtc gacgccgtcg cgcacgccga cagcctcggc
    37921 tggacactcg gggaacggct cgtggtcgtc gtcgccgaaa ccgacgagga cgactcggcg
    37981 accgagcggt ccggcgagga gatccgagcg ctgcagcagc ggttcgcccg cgcgtggacc
    38041 cgggccgtcg gcgctcgcga ccgtcgcgcg ccggtcatgg gtttctcgca ggaggtcgtc
    38101 accctgctcg cgatcccggc ggacgcgacc accgagaagg tgatggcgac gatcaccgat
    38161 ctggcgcggg tcgtccgcgg cgacggcggc ggtggccggc gcccgttctc caccggcgta
    38221 tcgcgaccgg tgacgggcgt ggagaacctc cccgcggcat acgccgaagc gctgcgggca
    38281 gtagcggtcg gacggcagaa gcacggcccg ggagcgatca cgcacttcga cgaacttggg
    38341 atctaccggc tgctctccct cgtgccggac agcgacgaac tgcgccagtt cgtcgacgac
    38401 gccctcggcg agctggccac cgacgacaac cccgagaacg cggggctgcg caccaccttg
    38461 accgtgctca tcgacaccaa tctcaatgtg gccgaggcca gccggatcct gttcttccac
    38521 tacaacacgc tgcggtatcg gatcgcgaaa ctggaacgga tgctcgggcc gttcacgacc
    38581 gatccgcagc tgcgcctgac actcgccctc gcgctgaaag tgctcgagat gcgcggcttg
    38641 tcccagaatc accaggcccg tccgcccgcg cgagtgtcac aacagggtta acacgctcgg
    38701 acacgcggtg aagaattgcc aacaaaaccg caggttggcc gtagtttttt gctcagtgcg
    38761 taaaacgcgc accaccacta ccgttccccc catccccaaa cgatggggtg agctgcgtca
    38821 tacagacgcc gattgaggtt ggagaagacc ccatggcatt cagttggact ctgcacgggg
    38881 acggcaacct gtccggcccc gatgacgtcg tcgcccccga cgaacgtctc tcctggggca
    38941 agaccatcgg tctcggtgcc caacacgtcg tcgccatgtt cggggcaacc ttcgtcttcc
    39001 cgctcatcat ggggctcaac ccgcagctcg cgatcatgat gagcggcctg gcgaccatca
    39061 tcttcctgct catcgtgggc ggccgggtgc cgtcctatct cggcacctcg gcctccttcg
    39121 tcggcgccgt cgcggccatt cgcgcgcagg agggcgccga ttcgtcccgg gtcaccggag
    39181 cgatcctcgt cgccggcctg gtcctcgcga tcatcggtgt cctgatccac ttcatgggtt
    39241 ccggagtgct gcaccgggtg ctgccgccgg tggtcaccgg tgcggtcgtc atgctcatcg
    39301 gcttcaacct ggcgccggtc gtcgccggca tctattggcc gcaggaccag tgggtcgcgc
    39361 tgctgacgat gctcttcacg atcatctgcg cggtcgcctt caagggcttc atcagccgga
    39421 tctcggtctt cctcgctctc atcttcggca ccctgctgtc ctggatcctc gacaagaccg
    39481 ccggcccgat cacctccgtc ctcggcggcg cgaccgaggc caccgagcac gtccgctgga
    39541 acacctccgg catcggcgat gccgcgtggt tcggcctgcc ggccaagacg atggtcggcc
    39601 cggacggcat ggaggtcgcc ggctggcaca tgccgagctt caacgcggcg gccatcctgc
    39661 tcgtcctgcc cgccgtcatc gccctcatcg ccgagaacac cggccacg
//
LOCUS       DAOPJG010000009        38752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1107_length_38752_cov_8.446546, whole genome shotgun sequence.
ACCESSION   DAOPJG010000009 DAOPJG010000000
VERSION     DAOPJG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 38752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38752
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1107_length_38752_cov_8.446546"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1035
                     /locus_tag="PKU75_01915"
     CDS             112..1035
                     /locus_tag="PKU75_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPF79661.1"
                     /translation="MRKLVLLALVGLAAQLVDGGLGMAYGVTTSTLLLAIGTNPAAAS
                     ATVHLAEIGTTLVSGLSHWRFGNVDWMVVVKVGIPGAVGAFLGATALSNLDADTAKPV
                     MSAILLVLGLYVLLRFTFKGVRTDKLGQRLRARFLAPLGLFAGFLDATGGGGWGPVGT
                     PALLASGRIEPRRVIGSISASEFLVTVAASLGFLLALGSEGINFTWVAALLLGGAIAA
                     PIAAWLARHVPARILGSAVGGLIILTNTRTILRSDWVDPSSATTWTIYGLIWAGWASA
                     IAWSVMQHRLDRTGGVTTDEENAALVGHSTH"
     gene            complement(1075..2163)
                     /locus_tag="PKU75_01920"
     CDS             complement(1075..2163)
                     /locus_tag="PKU75_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPF79662.1"
                     /translation="MGTALHLSGPVLVGPDEIRDEIWVIDGRISFTAPAAEPAGRIDG
                     WVLPGLVDAHCHVGLDKDGAVDAETAEAQITRDRDHGTLLIRDAGSPADTRWIDDRED
                     LPKLIRAGRHIARTRRYIRNYANEVEPDQLADAVREQARRGDGWVKIVGDWIDRDTGD
                     LTPCWPREAVAAALAVAHEEGARMTAHCFGPDVLHDLAAIGIDCVEHASGLEPETIES
                     FAAQGISIVPTLINIDNFPAIAEPAREKFPDYHRRMIDIYERRDQTIVAAYDAGVPIR
                     LGTDAGGTMSHGLAAREAQRLVVAGVPHTAALDAGTWGAREWLGRPGIEDGADADLLV
                     LPADPRADLAVLTAPTAVILRGVVVAGG"
     gene            complement(2202..3371)
                     /locus_tag="PKU75_01925"
     CDS             complement(2202..3371)
                     /locus_tag="PKU75_01925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPF79663.1"
                     /translation="MRVAIVTESFLPMINGVTTSVCRVAEQLAALGHDVVIVAPEPAP
                     AWFGPIPVHTVRSVPVRQFPTGVPSGRVEYLLAEIAPDVVHVASPFVIGARALTAAGE
                     LGLPSVAIYQTDMPRYVEQHSPGPVAPQVARLAWRWVRRIHALADVTLAPSHAAVQAL
                     QEHGVERVALWGRGVDTALFTPAWRSHPDTHTLRADLAPGGEVLVGYVGRLAPEKEAD
                     RLAELHGIPGIRLVIVGDGPSRPALEAEFRTLKWAGPAPVFLGFRTGEALAQAYAALD
                     LFVHPGTTETFGQTLQEAAASGLPVVAAAVGGPLDLVDHGRTGLLFDPARPGALGACV
                     GHLAGAPEERIAMGGRASEAVAARTWPALTDELVGHYRRAVSQAQERRLALDAAP"
     gene            complement(3604..4443)
                     /locus_tag="PKU75_01930"
     CDS             complement(3604..4443)
                     /locus_tag="PKU75_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPF79664.1"
                     /translation="MTSAGPLNTLAVGSAGPRIAFVHGLFGQGRNWNQIAKSVAWPNG
                     TQARALLIDLPDHGRSPWSEEFSYAAYADAVAATLRAAAPGEEWTLVGHSLGGKTSML
                     AALQHPDLIARLCVVDIAPKSYGDLHRFSAYIDGMQRLPLGEIATREEADDWFTAVEP
                     DPGVRAFLLQNLRRDHDGWSWRANLDLIAADAARGEQSRIADFPDTSGLAPYEGPVVW
                     LVGAESRYVTDDDSAPMRALFPQVRKVTVKGAGHWVHTDAPAVVIETLRRLLRTGGPP
                     PTS"
     gene            complement(4440..6041)
                     /gene="ffh"
                     /locus_tag="PKU75_01935"
     CDS             complement(4440..6041)
                     /gene="ffh"
                     /locus_tag="PKU75_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPF79665.1"
                     /translation="MFTSLSDRLTATFKSLRGKGKLSESDVNKTVRDIRMALLEADVA
                     LPVVKDFTQAVRERALSAEVSEALNPAQQVVKIVNDELVDILGQHTRPLRLAKTAPTV
                     IMLAGLQGSGKTTLAGKLAKWFKDQGDTPLLVAADLQRPNAVTQLEVVGQRAGVPVFA
                     PERGNVGGHDAVGPTGGGTRSYGDPVEVAEDGIQHAISKLYNVVIVDTAGRLAVDEEL
                     MEQAADIKDAIEPDEVLFVIDAMIGQAAVETAQAFQEGIDFSGVVLTKLDGDARGGAA
                     LSVAKITGRPIMFASTGEGLKEFEIFHPDRMASRILDMGDMLTLIEQAERAFDAKQAA
                     EMERKFLAAEDFTFDDFLQQMAAIKKMGSLKSMLKMMPGMNQIRDQLDAFDEREFDRI
                     EAMIRSMTPFERNHPKQINGSRRARIAAGSGVTVSQVNDLLVRFSEAQKMMKALAKGG
                     SIPGMPGMPGLAGGGKRGKQVQRKKGKSGNPAKRAEQERAAAQAASNRAPAGSAFGST
                     SSDPYANANLDPSQLPKGFEKFLGT"
     gene            complement(6100..8448)
                     /locus_tag="PKU75_01940"
     CDS             complement(6100..8448)
                     /locus_tag="PKU75_01940"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HPF79666.1"
                     /translation="MSRTTTDVSGARLDLAGTRAFTAPGAGQARRAAIAEFTTSWLRD
                     IWAEATGGRSLHGVALAAVGSLGRGDAGPLSDLDLVLLHNGRTLREKEINGLADRLWY
                     PVWDAKVRLDHSVRTLNQCRSVASDDLSAAVGLLDVAPVAGDPEVVSAIRATLSHDWR
                     ANARQRLPQLLENLAERHTRHGELAQLIEPDLKEARGGLRDMTVLRALAAAWLTDRPH
                     GAVDEAYARLLDVRDALQVVTGRGRDRLGREDHDAVAALLGYTDADDLLTMVSSSART
                     LAYALDATTRRAGQSQRARTLRVGPRAPHLVALGFGVFEHDGEAVLGRHAVSDALLPL
                     RAAVVAARSKLPLAPQTLANLAELPDLPTPWPEAARDLFTDLLATGEGLPGVWAGLTQ
                     AGLVRRWLPEWDGVDSRPQRNPVHRHTVDRHLIETVVQAGAMVREVARPDLLLLGALL
                     HDIGKVAGARDHSHAGARIAREVLARMGLPATDAAVVERLVAEHLTLIELATRRDIAD
                     PATAAAAWAAAGEDPDAFDLLRVLTIADAKAAGPLAWSDWRAALFANLVAAVGDQDRD
                     RALPPPEGRAELAAPVHEAIAQGQPYVEVSAVEGAFRVVVHDRDRVGLFADTAGLLAA
                     QGLTVRTAALRTVDGVAADEWVVEAVTGDAPERARLVHGLAALSRGERGPLAALERRR
                     TFARPRAAAVAEGMATRAFVVPGAAEGATVLEVRSRDRVGLLYDLGRCLAAAGLSVRS
                     AHIATYAGQTLDTFYLTEPRGGQLSPGRVAATVAAVIDCCDG"
     gene            complement(8685..9023)
                     /locus_tag="PKU75_01945"
     CDS             complement(8685..9023)
                     /locus_tag="PKU75_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPF79667.1"
                     /translation="MKLITAIIKPHQLEEVKAALEAYGIAGMTISEASGYGRQHGHSE
                     VYRGAEYTVDFVPKIRLEVLVEDMDSSSVVDVIVKSAATGRIGDGKVWVTHVEDVARV
                     RTGERGSDAI"
     gene            complement(9020..10420)
                     /locus_tag="PKU75_01950"
     CDS             complement(9020..10420)
                     /locus_tag="PKU75_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPF79668.1"
                     /translation="MTALHTLAAADEPTIDLASTGFVIICASLVLLMTVPGLALFYGG
                     MNRAKSVLNMMMMSFGAAGLVGVLYVLFGYSMSFGPSDIGGIFANPFDKLGLKDTAVL
                     VNPFGYEGYGNIPELAFVGFQLTFAIITVALISGAIADRVKFSTWLVFSGLWVTLAYF
                     PIAHMVWGGGLLSASESGLSARIFGSTDGAADVVPIDFAGGTVVHINAGMAGLVLALI
                     VGKRLGFGKTIMRPHNVPLVMIGAGLLWFGWFGFNAGSELAPDETAAMVWVNTVVATC
                     AAMLGWLLVEKIRDGHGTSIGAASGVVAGLVAITPACGALSPIGSIILGLIAGGLCAL
                     AVGLKYRFGYDDSLDVVGVHLVAGLWGTIGAGLLSTATGLFYGGGVQQTVIQIVIALA
                     SLLICGIVTAALGFALKATMGWRINEDDETAGIDQSEHAETAYDLSPRGGRMAGALGL
                     GSHAREEAPIAEGANA"
     gene            10578..10964
                     /locus_tag="PKU75_01955"
     CDS             10578..10964
                     /locus_tag="PKU75_01955"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HPF79669.1"
                     /translation="MTTRPTPVLVFDGDCAICSTLARFTARRVRRHDADFAIAPWQEL
                     DLASLGLTEAQCIEALQWIGADGKISAAHNAVAQALWNGHAWFKPAAAVIWAPGINQL
                     AAVVYRWVADNRHRLPGGTPACSIPR"
     gene            complement(10961..11815)
                     /locus_tag="PKU75_01960"
     CDS             complement(10961..11815)
                     /locus_tag="PKU75_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79670.1"
                     /translation="MNWLFPAVPLRRLALFRTMVYLYVVIDVLWLQTSGWYHGYADPV
                     WYEPLRMGQLLHLPAATVPLVNTLKWGSVAAALVAATGRLPRLAGSVVAICWIWYMYI
                     AYAYGKVDHDRGDFVIALLLLPTVGSAFAQARWGRTADGRDILSQAGGFVLRAVQIFA
                     IATYFLSAWAKVRFGGWDWVNSATMARAVVRRGTPWVKWLLDAPWLLQATQWALFAAE
                     MASPLIFLASERWRRRIVYAWFAFHAVTYASITIAFWPHLVMLLAFLPLEQYADRGKP
                     SSAASVVL"
     gene            complement(11812..12258)
                     /locus_tag="PKU75_01965"
     CDS             complement(11812..12258)
                     /locus_tag="PKU75_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79671.1"
                     /translation="MPLTLPQKAWRVAVVLLAAVLFLGGTFVGDDDWWPFSPWRMFST
                     STDPNGAVRSTFIEVRTAAAPATWVEAPISPDSVGLNRAEIEGNLDRIAKDPAMLKTL
                     ADSHTALRPDDPSWVGIRVVVRNYLLRDGGPTGQHRDDLVAEWRAS"
     gene            complement(12295..13482)
                     /gene="ftsY"
                     /locus_tag="PKU75_01970"
     CDS             complement(12295..13482)
                     /gene="ftsY"
                     /locus_tag="PKU75_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPF79672.1"
                     /translation="MDQLWEYLVVAGVLLVAIVGGLFAFLRPSRRRRPPAVPPTRPTT
                     PIPPATSDTEEPAGGGVDTLPAPPVEPEEDVLLEEPATPTLERPETARGRLQRLRARL
                     ARSNTALGKGLLALLTRGGLDEAAWEEVEDTLLAADLGVEASMELVESLRTQVQVDGT
                     TDEATVRTWLRTDLLALVDPGMDRSVASSRVEGRPAVVLVVGVNGTGKTTTVGKLARV
                     LVAEDKDVLLGAADTFRAAAADQLETWGARVGVPTVRSHKEGADPAAVAFDAVKAGIE
                     LEADVVLLDTAGRLHNKTNLMDELGKIKRVVEKQSPIDEVLLVLDATTGQNGLRQAEV
                     FSQVVGITGIVLTKLDGTAKGGIVVAVQRKLGVPVKLVGLGEGPDDLAPFDPEAFVDA
                     ILQ"
     gene            complement(13623..13901)
                     /locus_tag="PKU75_01975"
     CDS             complement(13623..13901)
                     /locus_tag="PKU75_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79673.1"
                     /translation="MLRVLLPLILCVLISLVVLALVAIPARRQGRDLLTARGEEVFVK
                     VKAGTDAAATKTTGLVTSAAARIRPTGGEEATPVSEAPHSSTESAPQR"
     gene            complement(14013..14468)
                     /locus_tag="PKU75_01980"
     CDS             complement(14013..14468)
                     /locus_tag="PKU75_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79674.1"
                     /translation="MKSMTRAAVAVASAGLMALGGASAANAAPDTGAVSDAKVTCYGK
                     ANKPFIIGGVGNTSRQVGVIYCAGGGAYIRGRVTIQQLVNGYWYDRGNATTQTKYVGY
                     NNVQFPDSHAARPGVWRTKATFTFAGGGASRTFYSASVRYVPARHGVTM"
     gene            complement(14630..18178)
                     /gene="smc"
                     /locus_tag="PKU75_01985"
     CDS             complement(14630..18178)
                     /gene="smc"
                     /locus_tag="PKU75_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPF79675.1"
                     /translation="MYVKTLTLKGFKSFASATTFRLEPGVTCIVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGKMEDVIFAGTSGRAPLGRAEVTMTIDNTDGALPIDYTEVTISR
                     TMFRGGGSEYAINGTHCRLLDIQELLSDSGIGREMHVIVGQGQLDTVLRATPEERRGF
                     IEEAAGVLKHRKRKEKALRKLETMEANLTRVADLTSEIRRQLGPLGRQAEAARKAAVI
                     QAEARDARLRLLADELAQLTSAMEQEAADETELLRRRAEVESALATARAAVADSEQQL
                     AAGGPVLAAERERWIALRTTAERLQSVHSLAEERLRLLRADDEDETTTGRDPEELLAQ
                     AQQAREQERDLLAEVAAAKDALVIARDAREAAEGDAAAEQRRVAAHARAVADRREGLA
                     KLAGQVGARRSRLEAAEAEIGRLTEAAAASLARAIAADQEFAALEGSIADEEEGEEGL
                     DAAYEAAAQRQEAARADLDRWVEEERTADRERTALTARLDALRLGLRRKDGAATLLGS
                     EEVATSGTLAQSLRVAPGDEVAIAAALGYAAEAVTVADLTAASAAVAHLRDQEGGRAT
                     ILVQGAPAPADPAPALPAGARWARASVTVPDALDGALTRLLDGVVLVADDAAARALVE
                     QSGDLTAVTPAGDVVTAAYVRGGSADAPSLIETQAAVDETEAELRSAATRLDQARFHV
                     AGARTALEQATAAADGHLERLHESDARMAAVAEQLGSLGSASRAARAEVERLERAREQ
                     AAAAMADDQAALAELTARFEAASAEPEESEEDFPDRTGELEAAATAARAGETEALLAL
                     RTREERARALSGRAASLEGAARNEIAARERLKARRERRRREAAVADSVGAAAAYGLLR
                     VSAATGRAEGAVAALDARAREQQTQLAALRVQLSAAQDDLAALTDDIHRDEVARAAQQ
                     ARVEQLQQKAIEELGIDPDVLVEDFGPHQLVPVVVAAGEEPGEPQPYVREVQEKRLRR
                     AERGLRELGRINPLALEEFAALEERHSFLTKQMEDLKASKRDLLDIVREVDQRVEEVF
                     TAAFHDTAEQFERVFARLFPGGEGRIFLTDPSDMLTTGIEVEARPPGKKVKRLSLLSG
                     GERSLVAVALLVAIFKARPSPFYIMDEVEAALDDTNLGRLITLFEELRDSSQLIVITH
                     QKRTMEVADALYGVSMRGDGVTNVVSQRLRSLDEPA"
     gene            complement(18282..18605)
                     /locus_tag="PKU75_01990"
     CDS             complement(18282..18605)
                     /locus_tag="PKU75_01990"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPF79676.1"
                     /translation="MTRGEPSGTDAGMPPELIRLRSSIDNIDAALVHLLAERFKATQQ
                     VGELKAAQGLPPADAAREERQIARLRALAHDAGLDPQFAEKFLNFIIAEVIRHHEAIA
                     ADSTD"
     gene            complement(18715..19032)
                     /locus_tag="PKU75_01995"
     CDS             complement(18715..19032)
                     /locus_tag="PKU75_01995"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPF79677.1"
                     /translation="MPDHHDPSAPEDAERVVVHVSGRVQGVGFRWWTRARALELGLNG
                     YARNTDDGRVEVCAEGAPDAVARLLALLSEYPSTTRRPGAVRAVSTPVRGAPRGYEGF
                     AER"
     gene            19143..21068
                     /locus_tag="PKU75_02000"
     CDS             19143..21068
                     /locus_tag="PKU75_02000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPF79678.1"
                     /translation="MPSDLEVAMTATVSPPGSATASSRPAPRAPSRVRSSAPSTGRLP
                     GLDGLRTVAIVAVLIFHLNAAWLPGGFIGVDIFFVVSGFLITTLLVRERASTGRVDLA
                     GFWGRRARRLLPALLLAIPVSVLLARLTEADLLVGVGSQVLGALTFAFNWVQIAAGAD
                     YFHATTPQLFMNYWSLAVEEQFYLIWPLATLVLLRLVRSGRARAGIAGGLAVLSAVLM
                     ALRFDPDAGATRVYYGTDTHVFGLMIGAALAFWYADGGAARLAAPAWVARRRPVAGIA
                     GAVLLGSMWWLEESRALTFRGGLAIASLATAALIAVAVGGGTVARLLDLPPMRWIGER
                     SYGIYLWHWPVILVVNADITSVPGSAGFLSSRLWAVLVTFAIADLSYRLVERPVRRFG
                     WRGAFTRLAACVPGRADGARVLAGAITVLVVATVAVIATAPDATSTEKLIAANAKAAE
                     RTATSTATAPALTTATFTMPKGNQIDAYGDSMMIGSVPALKYYFPGIRIDAKSNRRWS
                     AGLAAVTASGTDIRRAVVLSFGTNAGVDEADVRATLDKLGPKRMVVLVNIHLNEARAT
                     ADNTTLAKVAADYPNAIVADWNAEVTKQRGLLQPDGIHPSMTGQHRYAALVRQALADL
                     SYQHTGKVVKLKPLPIP"
     gene            21079..21321
                     /locus_tag="PKU75_02005"
                     /pseudo
     CDS             21079..21321
                     /locus_tag="PKU75_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit A"
     gene            complement(21571..22428)
                     /gene="mutM"
                     /locus_tag="PKU75_02010"
     CDS             complement(21571..22428)
                     /gene="mutM"
                     /locus_tag="PKU75_02010"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPF79679.1"
                     /translation="MPELPEVEVVRRGLAAHVTGARITDVETHGARVLRRHEPGAADF
                     AARLRGRTIREVARRGKYLWLDVGDEAVVVHLGMSGQMLLARPGDPDPRHLHARFGVT
                     TGEGDPIEVHFVDQRTFGGLALTAVDQDGIPEPVAHIARDPFDPLFDPLAVARRITKR
                     PSAIKRQLLDQTIVSGIGNIYADEALWRAKLHGERPGDRLTKPAVVRLLGHAHDVMSD
                     ALGEGGTSFDSLYVNVNGESGYFERSLNAYGRGGQPCRRCDTPMRREHFMNRSSFSCP
                     RCQPRPRRG"
     gene            complement(22434..23255)
                     /gene="rnc"
                     /locus_tag="PKU75_02015"
     CDS             complement(22434..23255)
                     /gene="rnc"
                     /locus_tag="PKU75_02015"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPF79680.1"
                     /translation="MQRPIDELRDHILGVCGVAVEGPLLTLALTHRSYAYESGAIPHN
                     ERLEFLGDSVLGIVVTDHLYRTYPDYTEGHLAKLRAATVNMRALADVGRTIGLGDFLF
                     LGRGEETTGGRDKTSILADTTEAVIGAVYLAGGMAAATVFVHHLVDPVLTASARLGAG
                     LDWKTSLQELSVTRDLGPIQYTIVGEGPEHEKVFTASALVGGTVHGVGVGRTKKDAEQ
                     QAAEQAWRALSAGPVIDAPEAVPVDGHVVEAQAVQAQAVQAHAGDVLTPQPHPAT"
     gene            complement(23272..23466)
                     /gene="rpmF"
                     /locus_tag="PKU75_02020"
     CDS             complement(23272..23466)
                     /gene="rpmF"
                     /locus_tag="PKU75_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPF79681.1"
                     /translation="MAVPKRKTSRSNTRSRRANWKATAVATTTCPQCKSPMVPHVACP
                     SCGTYKGRHYGAAERTEHQA"
     gene            complement(23468..24055)
                     /locus_tag="PKU75_02025"
     CDS             complement(23468..24055)
                     /locus_tag="PKU75_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HPF79682.1"
                     /translation="MNRLDPRSPMVFSTVELGRRPGSMVQVTREIESSEPIGTDVLAV
                     PAGAPLDVELRMESVLEGVLASGSVRATAVGACVRCLEPVTVDVDATFQELFAYPERA
                     AHNRKVGATDEETQEYEVQDDYLDLEPVLVDAVVPALPFQPVCRDDCPGLCPQCGFRL
                     ADDPAHAHELLDPRWSALADLATGADAADPTEKRN"
     gene            complement(24161..24655)
                     /gene="coaD"
                     /locus_tag="PKU75_02030"
     CDS             complement(24161..24655)
                     /gene="coaD"
                     /locus_tag="PKU75_02030"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPF79683.1"
                     /translation="MTSRVVCPGSFDPVTNGHLDVIIRAAALFDEVVVAVMHNPAKSG
                     TFPVEQRIAFIEEATAGTSGVRVLAFTPRLIVDVCRDLEATALLKGLRGEVDFAYEVP
                     MASMNRHLTGVETLFLPGDPAVAHISSSLVKEVCALGGDVTGLVPDPVRDALVARRDG
                     SRSS"
     gene            24705..>24966
                     /locus_tag="PKU75_02035"
     CDS             24705..>24966
                     /locus_tag="PKU75_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPF79684.1"
                     /translation="MARRTLDLLTGAARADELAAVLDDLVAAARQILGDRFVGAYLHG
                     SLAQGDADLESDADFLIITDGPVDEEQFVALARLHHRLAHGES"
     assembly_gap    24967..25066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25067..25482
                     /locus_tag="PKU75_02040"
     CDS             <25067..25482
                     /locus_tag="PKU75_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HPF79685.1"
                     /translation="DTIPARVILHENGIALAGPSATTVVPPVPGDLVVADAAARLAEW
                     LPDLDEWAFTDAWSQRYSVLNLARMVAAHETGQVLSKRAGAGWAATLRGGEFAPLLEA
                     MVAARGATAFDDPADPAWIPQQHAFFAAVAARSGS"
     gene            complement(25678..26232)
                     /gene="rsmD"
                     /locus_tag="PKU75_02045"
     CDS             complement(25678..26232)
                     /gene="rsmD"
                     /locus_tag="PKU75_02045"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPF79686.1"
                     /translation="MTRIIGGRVGGRTIAVPRGAATRPTSDRVREALFSMLVARDVIA
                     GARVLDLYAGSGALGLEAVSRGAASADLVEADRPTARLIERNARDLGIPARVHAMTVR
                     AFARRPVVAPADLVLADPPYPLAEADLAADLAALVDAGWLAPGALLVLERSSRSPEPC
                     WPDGMTATGSRRYGETTLWLAEAG"
     gene            complement(26229..28427)
                     /locus_tag="PKU75_02050"
     CDS             complement(26229..28427)
                     /locus_tag="PKU75_02050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPF79687.1"
                     /translation="MAHLDSNLAPLIASAARHLRNARGVVTVGDLLRMRPTRYLSYDS
                     EVTELRDGEFVVLIGTIKSAHTRSMSTRKGSMLTAVLTDGHQDVDMTFFNARAHENKL
                     LPGVRVIAGGQVGTYNHRRQLAHPGYTLLDEAAEAALPGLYPVYPAIPKLHNWNLVQA
                     VGLVLDALGPLPDPLPAPLRSRHRLPGLGETLRMLHRPETRAEVARARERLRYEEALV
                     LQTVLAQRRVQRAQEEAALAIAEGGPLLTAFTDRLPFTLTAGQEQVAGEIAADLASTV
                     PMNRLLQGEVGSGKTVLALRAMLSAVDGGAQAALLAPTEVLATQHHRSITAMLGDLAE
                     GGLLGGSEIGTRVVLLTGSQGSASRRRALADVVSGDAGIVIGTHALLQENVAFYNLGL
                     VVVDEQHRFGVEQRDVLRGKASGTPHLLVMTATPIPRTIAMTVFGDMETSTLTELPAG
                     RSPIQTFVVDEHRPGWLERTWARIGEECRAGRQAYVVCPRIGPGASGGESGGESGGDE
                     EGEVDWFSDGFGQGEQAPARPEMIGVLETFEELSTEASLAGLRIGLLHGKLPTEEKDA
                     VMRAFAAGELDVLVATTVIEVGVDVPNATVMVVRDADRFGVSQLHQLRGRVGRGGHPG
                     LCLLATRAADGPAGERLTAVAATTDGFELAQLDLRQRREGDILGARQSGGRGRLQFLS
                     LAHDEETIATARADAAELIAQDPDLSGHPELAGVIEEWLRREQADFLEKT"
     gene            28628..28822
                     /gene="rpmB"
                     /locus_tag="PKU75_02055"
     CDS             28628..28822
                     /gene="rpmB"
                     /locus_tag="PKU75_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPF79688.1"
                     /translation="MAATCDVCGKGPGFGHSISHSHRRTKRRWNPNIQRVRAMVGEAG
                     KTPKRLNVCTSCLKAGKVSR"
     gene            complement(28893..29888)
                     /gene="thiL"
                     /locus_tag="PKU75_02060"
     CDS             complement(28893..29888)
                     /gene="thiL"
                     /locus_tag="PKU75_02060"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPF79689.1"
                     /translation="MSSSPQGVVADLTEDRLLAQVLPLLAGSRTERVGPGDDAAVLDV
                     ASPLIATTDTMVLGRDWRDDWSNAEQVAAKAVVQNLADIAAMGAVPTTLLLTLVLDPQ
                     TPLAWVLDFARALGEQCRRYAVSVAGGDLSSAPAGVKMVTITALGELAGAEPVLRSGA
                     QPGDVLAISGPLGRSAVGLALLERAASGTALDAREEAALAHHRSPSAPLSQGPIAARA
                     GASAMLDVSDGLSRDAARIARASGCLIEIDSATLAPDLNWAEGLVPRDQARACVLNGG
                     EEHSLLATFPDRASVPEYWRILGRVEAPAAGEDPGVHLDGRPLTEAGWDHFHPAR"
     gene            29981..30214
                     /locus_tag="PKU75_02065"
     CDS             29981..30214
                     /locus_tag="PKU75_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPF79690.1"
                     /translation="MVQAYILIQTEVGKSASVTEAVSGISGVTLAEDVTGPYDCIARV
                     EAGTVDDLGKLVIAKIQEVPGITRTLTCTVVHV"
     gene            30201..30761
                     /locus_tag="PKU75_02070"
     CDS             30201..30761
                     /locus_tag="PKU75_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79691.1"
                     /translation="MSTSEPNPGYAARPPERWGELLLTSPGRWGLAGLVAAPLLLAGC
                     SPGGVDVSPAAQAEAAACQSVAWPDRLGELARTASPDDPSLAAWGDPPVIARCGIPAL
                     TPTTDECIAVNDVDWVLRKLDDGYAFTTYGTDPGLEVLVPDDYAPETMVLVDFTEIAK
                     GLPRSGRACLAVRAVGASVPSGAPSG"
     gene            complement(30768..31901)
                     /locus_tag="PKU75_02075"
     CDS             complement(30768..31901)
                     /locus_tag="PKU75_02075"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPF79692.1"
                     /translation="MTSTSDPSGKLRVVLLFGGRSSEHVVSCATAAGVMRAIDPQRYE
                     IVPIGIAPDGKWVLLDNDPTPLELTRDHTPQVPEDAATVLVPTSTRDRGLSVHDAGEV
                     PRELGEVDVVFPLLHGPYGEDGTVQGLLELADIRYVGSGVLASAAGMDKHFMKVVFAG
                     AGLPVGPHTVITDAQWRHDPALARDAVAGLGMPVFVKPCRAGSSMGISKVTSMDQLDA
                     AVEEARRHDLKVIVEAGMDGREIECAVLQGHDGAPPRTSEVGEIVVREGHGHDFYDFD
                     AKYLDADAVELSCPADVPPEVSDRIRELAVRAFMALGCEGLARVDCFYTRAGDVIVNE
                     INTMPGFTPSSMYPRMWQASGVSYPELIDELIALAMERPVGLR"
     gene            31983..33062
                     /locus_tag="PKU75_02080"
     CDS             31983..33062
                     /locus_tag="PKU75_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPF79693.1"
                     /translation="MPAARAPRRPATAVVAAGRPPAEAGAGLTVPPHFTSTYTAGGPV
                     VYARQGNPTWSAFEEVVATLEGGRALVFASGMAAVDAALSALPHGAVVVAPAGAYNGV
                     DSVLREGEAAGRWQVRWVDVTDTAAIAGAVAGADLLWLESPTNPLLDVADVPAAIAAG
                     RAAGALVVVDNTFATPLLQRPLESGADIVLHSATKYLSGHSDLILGVLVVAVEDEQRY
                     AALARRRTLGGGIPGPMDVWLATRGIRTLSVRFERSCRNAAILAARLDRHEAIERVRY
                     PGFGAMVSVEIRGGSGAADAVCAAASLISNSTSLGGVETQWERRRAIPNEPETTPVNL
                     VRISVGIEDVEDIWADVEQALTRAG"
     gene            complement(33117..33872)
                     /locus_tag="PKU75_02085"
     CDS             complement(33117..33872)
                     /locus_tag="PKU75_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPF79694.1"
                     /translation="MIRTPDQRRLPGAYRFVVRVLRPVLQVLTKRDWRGVANIPAEGG
                     FVVCPNHLSHIDVLILGHFMYDAGRPVYFLGKESVFRVPVVGWIVTRAGQIPVHRGTH
                     RVADAYRSAVAAVRAGKAVGVYPEGTLTRDPDLWPMRGKTGAARIALETRCPVIPVAM
                     WGPNELLAPYTKLPKLLPRKTMGVIAQPPVDLSDLYDAPDRRAAVAEATRRIMASLTN
                     GVEQLRGQVAPVERYDPAHYGESEFGHPDKKAG"
     gene            33911..34555
                     /gene="cofC"
                     /locus_tag="PKU75_02090"
     CDS             33911..34555
                     /gene="cofC"
                     /locus_tag="PKU75_02090"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HPF79695.1"
                     /translation="MDEDLECVVIVPVQGAPHGKSRLTVPGRAGLSRAFAADTIDAVM
                     RVPSAHLVVVTPDPVLTRYARAAGALVVLDTGAGLNAAVAAGMAAGVPFGALPMAAML
                     GDLPAVTPEAVRSGLMQCAGHERAFVPDLAGTGTVLLWSRDGRLQPAFGPGSAAAHEA
                     LGYVRCERVPSHLRLDVDVRADLAVAIELGCGRHTAAALAAGALSHPVSHEGLG"
     gene            34561..34782
                     /locus_tag="PKU75_02095"
     CDS             34561..34782
                     /locus_tag="PKU75_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79696.1"
                     /translation="MQASVHRYDAADASGAVILDNGVVLPFTSDALRDSGLRGLRVGQ
                     RLSMEVTDADPPTVTRLWIVGIGEGQSIG"
     gene            complement(34873..35391)
                     /locus_tag="PKU75_02100"
     CDS             complement(34873..35391)
                     /locus_tag="PKU75_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPF79697.1"
                     /translation="MNKADLVQAIEGKLGSRKAATDAVETILETIVAEVSKGGKVALT
                     GFGTFEKAARAARTGRNPRTGETVKIRKTSVPRFTASAKFKDYVKNPKSLPKSAPLAR
                     ASSAAAKTAAAPAKSTARKAAPANAAATKAAPAKAAAKTATKAAAKKTATTKAAATKA
                     PAKKAPARKSAR"
     gene            complement(35560..36153)
                     /gene="leuD"
                     /locus_tag="PKU75_02105"
     CDS             complement(35560..36153)
                     /gene="leuD"
                     /locus_tag="PKU75_02105"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPF79698.1"
                     /translation="MEAFTSHTGIGVPLRRSNVDTDQIIPAEYLKRVTKSGFEDGLFA
                     AWRHDDSFILNNADYAGGSVLVAGPDFGTGSSREHAVWALMNYGFRVVISSRFADIFR
                     GNSGKQGLLTAVVSQDDVELLWKYLENEPGASVTVDLASRTITAGDIVAGFEVDDYTR
                     WRLLEGLDDIGLTLRHEPDITAYEGNRPAFKPVVAPG"
     gene            complement(36155..37579)
                     /gene="leuC"
                     /locus_tag="PKU75_02110"
     CDS             complement(36155..37579)
                     /gene="leuC"
                     /locus_tag="PKU75_02110"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPF79699.1"
                     /translation="MAGTLAEKIWADHVVRSAEGEPDILYIDLHLIHEVTSPQAFDGL
                     RLAGRPVRRPDLTLATEDHNVPTTPGPITDPVSLTQVETLRRNCEEFGVTIYPMGDAE
                     QGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPLKP
                     FKTMAVNVDGDLADGVTAKDIILAVIAKIGTGGGQGYVLEYRGEAIRNLSMEARMTVC
                     NMSIEAGARAGLIAPDRTTYDYLQGRDHAPTGAEWEAAVGYWETLRTHDDAVFDAVVD
                     IDAATLTPFVTWGTNPGQGAPLGASVPDPALIPDETEARAAEAALAYMGLTAGTPLRE
                     IAVDTVFVGSCTNGRIEDLRAAAAVLKGRKVADGVRMLVVPGSARVRLEAEAEGLGEV
                     FEAAGAEWRLPGCSMCLAMNPDKLAPQERSASTSNRNFEGRQGKGGRTHLVSPLVAAA
                     TAVRGTLSSPADLAPLATATGEEH"
     gene            37717..38472
                     /locus_tag="PKU75_02115"
     CDS             37717..38472
                     /locus_tag="PKU75_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPF79700.1"
                     /translation="MDNSSGVGVLDKAAVVLGALESGPATLAQLVTATGLARPTAHRL
                     AVALEHHRLVARDLQGRFVLGPRLGELAASAGEDRLLAAAGPVLGALRDHTNESAQLF
                     RRQGDYRVCVSAAERPVGLRDSIPVGATLSMHAGSAAQVLLAWEEPDRLHRGLHGAAF
                     NATTLSGVRRRGWAQSVGEREPGVASVSAPVRSPSGRVIAAVSISGPIERLSRQPGRL
                     HAATVIAGANKLTEVLARHAAAQEAAAARAAAH"
     gene            complement(38556..38628)
                     /locus_tag="PKU75_02120"
     tRNA            complement(38556..38628)
                     /locus_tag="PKU75_02120"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38592..38594),aa:Glu,seq:ctc)
BASE COUNT         5404 a        13775 c        13750 g         5723 t
ORIGIN      
        1 cccgcccgcg gcagcccgtc gccacgggga cttgcgcgca acccggaccg gagttaaagt
       61 catgccgctt tcctattaaa ccaatgggat ttacctttct aggggatgat tatgcgcaag
      121 ctcgtcctgc tggccctggt gggcctggcc gcccaactcg tcgacggcgg cctcggcatg
      181 gcctacggcg tcacgacctc gacactgctg ctcgcgatcg gcaccaaccc ggccgccgcc
      241 tcggccacgg tccacctcgc cgagatcggc accaccctcg tctccggact cagtcactgg
      301 cgcttcggca atgtcgactg gatggtcgtc gtcaaggtcg gcatccccgg cgcggtcggc
      361 gccttcctcg gcgcgacggc cctgtcgaac ctcgatgccg acacggccaa gccggtcatg
      421 tccgcgatcc tgctggtcct gggcctgtac gtgctgctgc gcttcacgtt caagggcgtg
      481 cgcaccgaca aactcggcca gcggctgcgc gcccgcttcc tggccccgct cggcctgttc
      541 gccggcttcc tcgacgccac gggcggcggc ggctggggcc cggtcggcac gccggccctg
      601 ctcgcgagcg gccgcatcga gccgcgccgg gtcatcggct ccatctcggc gagcgagttc
      661 ctcgtgaccg tcgccgccag cctgggcttc ctgctcgcgc tcggatcgga gggcatcaac
      721 ttcacctggg tggctgccct cctcctcggt ggcgccatcg ccgccccgat cgccgcctgg
      781 ctagccaggc atgtccccgc ccgcatcctc ggctccgccg tcggtggcct gatcatcctg
      841 accaacaccc gcacgatcct gcgatcggac tgggtcgacc cgtcatcggc gacgacgtgg
      901 acgatctacg gcctcatctg ggccggctgg gccagcgcca tcgcctggtc ggtgatgcag
      961 caccgcctcg accgcaccgg gggcgtcacc accgacgagg agaacgcggc gctcgtcgga
     1021 cactccaccc actgacatac gacccggctg accggcgagt ggcgatcccg ccggtcagcc
     1081 gcccgcgacc accacgccgc gcaggatcac cgcggtgggc gcggtgagga ccgcaaggtc
     1141 ggcgcggggg tctgcgggca gcacgagcag gtcggcgtcg gcgccgtcct cgatccccgg
     1201 ccgcccgagc cactcccgcg ccccccaggt gcccgcgtcg agcgcagccg tatgcggcac
     1261 ccccgccacc acgagccgct gcgcctcccg cgcggcgaga ccgtggctca tcgtcccgcc
     1321 ggcgtcggtg ccgagccgga tcggcacccc cgcgtcgtat gccgcgacga tcgtctgatc
     1381 gcgccgctcg tagatgtcga tcatccgccg gtggtagtcg gggaacttct ctcgcgccgg
     1441 ctcggcgatc gcggggaagt tgtcgatatt gatcagcgtg gggacgatcg aaatcccttg
     1501 ggccgcaaag gactcgatgg tctccggctc cagcccgctc gcgtgctcca cgcagtcgat
     1561 gccgatcgca gcgaggtcat ggaggacatc cgggccgaag cagtgcgccg tcatccgcgc
     1621 accttcctcg tgcgccacgg ccagggcagc ggccactgcc tcccgaggcc agcacggcgt
     1681 cagatccccg gtgtcgcgat cgatccagtc gccgacgatc ttgacccaac cgtccccgcg
     1741 acgggcctgc tcgcggacgg cgtcggccag ctggtcgggc tcgacctcgt tggcgtagtt
     1801 gcggatgtaa cgccgggtcc gggcgatatg ccggccggcg cggatgagct tgggcaggtc
     1861 ctcccggtcg tcgatccacc gggtgtcggc cggcgacccg gcgtcgcgga tcaacagcgt
     1921 cccgtggtct cggtccctgg tgatctgcgc ctcggccgtc tccgcgtcga cggccccgtc
     1981 cttgtcgaga ccgacgtggc agtgcgcgtc caccagtccc ggcaggaccc agccgtcgat
     2041 gcgcccggcc ggttccgccg ccggcgccgt gaagctgatc cgcccgtcga tgacccagat
     2101 ctcgtcccgt atctcgtccg gcccgacgag gaccggtccg ctcaggtgca gcgctgttcc
     2161 catgacgcag agcctagagc ggcccgtcac cggggcggat ctcagggcgc ggcatccagg
     2221 gccagtcgcc gctcctgcgc ctggctgacg gcccggcggt agtgcccgac cagctcatcc
     2281 gtcagggccg gccaggtccg ggcggcgacc gcctcgctcg cgcgcccgcc catggcgatg
     2341 cgctcctcgg gcgcccccgc gagatgaccg acgcacgcgc ccagagcccc gggccgggcc
     2401 gggtcgaaga gcaggccggt ccggccgtgg tcgaccaggt ccagcggccc gccgaccgct
     2461 gccgcgacga ccggcagccc cgacgcggcc gcctcctgca gcgtctggcc gaaggtctcc
     2521 gtggtgcccg gatgcacgaa gaggtccagc gcggcatacg cctgcgccaa cgcctcaccg
     2581 gtgcggaagc cgaggaagac cggcgccggc cccgcccact taagagtgcg gaactcggct
     2641 tccagggccg gccgggaggg tccgtcgccg acgatgacga ggcggatccc gggtatgccg
     2701 tgcagttcgg cgagccggtc ggcctccttc tcgggagcga ggcgaccgac atacccgacg
     2761 aggacctcgc cgccgggggc gaggtcggcg cgcagcgtgt gcgtgtccgg gtggctgcgc
     2821 cacgccgggg tgaacagcgc cgtgtcgacc ccgcgccccc acagcgcgac ccgctcgacg
     2881 ccgtgctcct gcaaggcctg cacggcggca tgcgagggcg ccaaggtgac atccgcgagc
     2941 gcatgtatcc ggcgcaccca ccgccaggcc agccgcgcga cctgcggcgc gacggggccg
     3001 gggctgtgct gctcgacata ccgcggcatg tcggtctgat agatcgccac gctcggcaga
     3061 cccagttcgc cggccgcggt cagggcccgg gcaccgatga cgaaggggct ggccacgtgc
     3121 acgacatcgg gagcgatctc ggcgagcagg tattcgacgc gcccgctcgg cacgccggtc
     3181 gggaactggc gcaccgggac ggaccggacc gtgtggacgg ggatcgggcc gaaccacgcc
     3241 ggcgccggct caggcgcgac gatgacgacg tcgtggccga gcgcggccag ttgttcggca
     3301 acccggcaca cgctcgtggt cacgccattg atcatcggga ggaaggactc ggtcacgatg
     3361 gcgactcgca catttccagg gtcgccgggt cagtgcatcc gcttcggggc cggcgcgggt
     3421 gtggcaggtg tgaaacaggt gaacttcagg acaccccgcg aggtttccgc atacgcagca
     3481 ggcgggaccc gactccgtcg ccgttccggg acttcgcggg gaccctgtcc cggggtctcg
     3541 ccccaggacc ttaggactcg cgtggccctg ggtatacgca aagccctagg aacacgcagt
     3601 gccttaggaa gtcgggggcg gtccgccggt gcgcagcagc cggcgcaggg tctcgatgac
     3661 gacggcgggg gcgtcggtgt gcacccagtg gccggcgcct ttgacggtca ccttgcgcac
     3721 ctgcggaaac aaggcccgca tcggtgcgct gtcgtcgtcc gtgacatagc gggactcggc
     3781 gccgacgagc cagacgaccg gtccttcgta gggcgcgagg ccggaggtgt cggggaagtc
     3841 cgcgatccgc gactgctcgc cgcgggcagc gtccgccgcg atgaggtcga ggttggcccg
     3901 ccaggaccaa ccgtcgtgat cgcggcggag gttctgcagc aagaaagccc gcacccccgg
     3961 gtcgggctcg acagcggtga accagtcgtc cgcctcctcc cgggtggcga tctctcccaa
     4021 gggaagtcgc tgcataccgt cgatataggc ggagaagcgg tgcaggtcgc cgtagctctt
     4081 gggcgcgatg tcgacgacgc acagcctggc gatcaggtcg ggatgctgca gcgcggcgag
     4141 catcgacgtc ttgcccccga gcgagtgccc gaccagcgtc cactcctccc ccggcgcggc
     4201 ggcgcggagc gtggcggcga cggcgtcggc gtatgcggca tacgagaact cctccgacca
     4261 cggcgagcgg ccatggtcgg gcaggtcgat cagcagcgcc cgggcctgcg tgccattggg
     4321 ccaggcgacc gacttggcaa tctggttcca gttgcggccc tgcccgaaca gcccgtgcac
     4381 gaacgcgatc cgcggccccg ccgacccgac ggccagggtg ttgaggggac cggcggacgt
     4441 cacgtgccga ggaacttctc gaagcccttg ggcagctggc tcgggtcgag gttggcgttg
     4501 gcataggggt cggaactggt ggagccgaag gcgctgcccg ccggcgcccg gttggacgcc
     4561 gcctgggccg cggcccgctc ctgctcggcg cgcttggccg ggttgcccga cttgcccttc
     4621 ttgcgctgga cctgcttgcc gcgcttgccc ccgcccgcga gcccgggcat acccggcatc
     4681 ccggggatgg agccaccctt ggcgagcgcc ttcatcatct tctgggcctc gctgaagcgg
     4741 acgagcaggt cgttgacctg gctgaccgtg accccggaac cggcggcgat gcgggcgcgg
     4801 cgcgagccgt tgatctgctt ggggtggttg cgctcgaaag gcgtcatcga gcggatcatc
     4861 gcctcgatcc ggtcgaactc gcgctcgtcg aaagcgtcga gctggtcgcg gatctggttc
     4921 atgccgggca tcatcttgag catcgacttc agcgagccca tcttcttgat ggcggccatc
     4981 tgctgcagga agtcgtcgaa cgtgaagtct tctgccgcaa ggaacttgcg ttccatctcg
     5041 gcggcctgct tggcgtcgaa cgcccgctcg gcctgctcga tgagggtgag catgtcgccc
     5101 atgtcgagga tgcgcgaagc catccggtcg gggtggaaga tctcgaattc cttcagaccc
     5161 tccccggtgg aggcgaacat gatcggccgg ccggtgatct tggcgaccga cagggccgca
     5221 ccaccgcggg cgtcaccgtc gagtttggtc agcacgaccc cggagaagtc gatgccctcc
     5281 tggaaggcct gggcggtctc gacggccgcc tggccgatca tcgcgtcgat gacgaagagg
     5341 acctcgtcgg gctcgatggc gtccttgatg tccgccgcct gctccatcag ctcttcgtcg
     5401 acggcgagcc ggccggcggt gtcgacgatg acgacgttgt acagcttgga gatggcgtgc
     5461 tggatgccgt cctcggcgac ctcgaccggg tcgccatagg agcgcgtccc cccgcccgtc
     5521 ggcccgacgg cgtcgtgacc gccgacattg ccgcgctccg gcgcgaacac cggcacgccg
     5581 gcccgctggc cgacgacctc gagctgggtg acggcgttgg gacgctgcag gtcggcggcg
     5641 acgagcagcg gggtgtcgcc ctggtccttg aaccacttgg cgagcttgcc ggcgagcgtg
     5701 gtcttgcccg aaccctgcag gccggcgagc atgatgacgg tcggtgccgt cttggccagg
     5761 cgcaacggcc gggtgtgctg gccgaggatg tcgacgagtt cgtcgttgac gatcttgacg
     5821 acctgctggg ccgggttgag cgcctcggac acctcggcgg acagcgcccg ctcgcggact
     5881 gcttgggtga agtccttgac gaccggcagc gcgacatcgg cctcgagcaa cgccatccgg
     5941 atgtcgcgca cggtcttgtt gacatcggac tcgctgagct tgcccttgcc gcgcagggac
     6001 ttgaaggtcg cggtcaggcg atcggagagc gaggtgaaca ccgtaaaagc ctaactgcgc
     6061 atcccgtgtg ccgcccattc gtgcggtcgc gaggccggct cagccgtcac agcaatcgat
     6121 gacggccgcc accgtggcag ccacccggcc cggagacaac tgcccaccgc ggggttcggt
     6181 cagatagaag gtgtcgagag tctggccggc atacgtggcg atgtgcgccg accgcacgga
     6241 caaccccgcc gccgcgagac accgccccag gtcgtacagc aaccccacgc ggtcgcgcga
     6301 gcgcacctcc agaaccgtcg ccccctccgc ggctcccggc acgacgaagg cgcgagtcgc
     6361 cataccctcg gccaccgcag cggcgcgcgg ccgcgcgaag gtgcgccgac gctccagcgc
     6421 cgcgagcgga ccacgctccc cccgggagag cgcggccaac ccgtggacca ggcgggcccg
     6481 ctcgggcgca tcgcccgtga cggcctcgac gacccattcg tccgccgcga caccgtccac
     6541 ggtgcgcagc gccgccgtcc gcaccgtcag tccctgcgcc gcgagcaggc cggccgtgtc
     6601 ggcgaacagg cccacgcggt cgcggtcatg aaccacgacg cggaaggcgc cctccacggc
     6661 cgacacctcg acatacggct gcccctgcgc gatcgcctcg tgcaccggcg ccgcgagctc
     6721 cgcccgcccc tccgggggcg gcagtgcccg atcgcggtcc tgatcgccaa cagccgcaac
     6781 gagattcgcg aagagggctg cacgccagtc ggaccaggcc agcggcccgg ccgctttggc
     6841 gtccgcgatc gtgaggaccc gcagcaggtc gaaggcgtcc gggtcctccc ccgccgccgc
     6901 ccaggccgcg gccgcggtgg cggggtccgc gatgtcccgc cgcgtagcga gctcgatcag
     6961 cgtcaggtgc tcggcgacca gccgttccac caccgccgca tcggtggccg gcagccccat
     7021 ccgggccagc acctcgcggg cgatgcgcgc acccgcgtga ctgtggtcgc gagcgcccgc
     7081 gaccttgccg atgtcgtgca gcagggcacc gaggagcaac aggtcgggcc gcgccacctc
     7141 acgcaccatc gcccccgcct gcacgaccgt ctcgatcagg tgccggtcga cggtatgccg
     7201 gtgcaccggg ttgcgctgcg ggcgcgagtc cacgccgtcc cactcgggca gccaccggcg
     7261 gaccaggccg gcctgagtca gcccggccca gactcccgga agcccctcgc cggtggcgag
     7321 caggtcggtg aaaaggtcac gcgcggcctc cggccagggc gtgggcaggt cgggcagttc
     7381 ggcgagattg gccagggtct gtggagcgag gggcagcttg ctgcgggccg cgacgacggc
     7441 cgcccgcagc ggcagcaggg catcggagac ggcgtgccgg ccgaggaccg cctcgccgtc
     7501 gtgctcgaaa acgccgaacc cgagggccac caggtgcggc gctcgcggtc cgacccgcag
     7561 ggtccgggcc cgttggctct ggccggcccg ccgggtcgtg gcgtccagcg cgtaggcgag
     7621 ggtgcgcgcg ctgctgctga ccatggtcag gagatcgtcg gcgtcggtgt agccgagcag
     7681 cgccgcgacg gcgtcgtggt cctcccggcc gagccggtcg cggccgcggc cggtgacgac
     7741 ctgcagggcg tcgcggacgt cgagcagccg cgcatacgcc tcgtcgacgg ctccgtgggg
     7801 gcggtcggtc agccaggcgg cggcgagcgc ccgcaggacc gtcatgtccc gcaggccccc
     7861 gcgggcctcc ttcaggtccg gctcgatgag ctgcgccagt tcgccgtgcc gggtatgccg
     7921 ctcggccagg ttctcgagca gctgcggcaa ccgttggcgg gcgttggcgc gccagtcgtg
     7981 gctgagggtg gcacggatcg ccgagaccac ctcggggtcg cccgcgacgg gcgccacgtc
     8041 gaggagcccg acggcggcgc tcagatcgtc ggaggcgacg gaccggcact ggttcagggt
     8101 gcgcacgctg tggtcgaggc ggaccttggc gtcccagacc ggataccaca gccggtcggc
     8161 gaggccgttg atctccttct cccgcaacgt ccttccgttg tggaggagga cgaggtcgag
     8221 atcgctgaga gggccggcat ccccgcggcc gagggacccg acggccgcca gggcgacccc
     8281 gtgcaagctc cgcccgcccg tcgcctcggc ccagatgtca cgcagccacg acgtcgtgaa
     8341 ctccgcgatg gcggcccggc gcgcctggcc ggcacccggc gccgtgaagg cgcgggtgcc
     8401 ggccagatcg agccgggccc cgctgacatc ggtggtggtg cgagacatgg tgacggcctg
     8461 tcctgtctgt gagctgtcgc gtgggcggga tggcgcgtcc ggagcgtggg cggcgggccg
     8521 acaaccgcgg cgcccaacgg tggcgtccgg gccttcggcg gcgcttccca cgcggttacc
     8581 catcgggccg tggggtgaac acccccactc ccgatggata gccacgtcgt tgacctcgga
     8641 ccggcgcgac ggccaggcca cggggccgtg gtgggatggg ccgatcagat ggcgtcggag
     8701 ccgcgttcgc cggtgcggac ccgggcgacg tcctccacgt gggtcaccca gaccttgccg
     8761 tcgccgatcc ggccggtcgc ggcggacttg acgatcacgt cgacgacgga cgaagagtcc
     8821 atgtcctcca cgagcacctc gagccggatc ttcgggacga agtcgacggt gtattcggcg
     8881 ccgcggtaga cctcggagtg gccgtgctgg cggccgtagc cgctggcctc gctgatcgtc
     8941 atacccgcga tcccgtaggc ctcgagcgct gccttgacct cctcgagctg gtggggcttg
     9001 atgatggcgg tgatcagctt catgcgttcg ctccttcggc gatcggggct tcctcgcgag
     9061 cgtgggagcc gaggccgagg gccccggcca tccggccgcc gcgcggagac aggtcgtatg
     9121 cggtttcggc gtgctcgctc tggtcgatgc cggcggtctc gtcgtcctcg ttgatgcgcc
     9181 agcccatggt ggccttgagc gcgaatccga gggccgcggt cacgatgcca cagatgagca
     9241 gcgaggccag ggcgatgacg atctggatga cggtctgctg caccccgccg ccgtagaaga
     9301 gaccggttgc cgtggacagc agcccggccc cgatggtgcc ccacaggccc gcgacgaggt
     9361 ggacgccgac gacgtcgagg gagtcgtcat acccgaagcg gtatttcagg cccaccgcga
     9421 gggcgcagag gccaccggcg atcaggccga ggatgatcga accgatcggc gagagggcac
     9481 cgcacgccgg ggtgatggcg accaggccgg cgaccacacc ggaggcggcg ccgatcgagg
     9541 tgccgtgccc gtcgcggatc ttctccacca gcagccagcc gagcatcgcc gcgcaggtgg
     9601 cgacgacggt gttgacccac accatggccg cggtctcgtc cggcgcgagt tcggatccgg
     9661 cgttgaaccc gaaccagccg aaccacagca gcccggcgcc gatcatcacg agcggcacat
     9721 tgtgcggacg catgatcgtc ttgccgaagc cgagacgctt cccgacgatc agggcgagga
     9781 ccaggccggc cataccggcg ttgatgtgca cgacggtgcc gccggcgaag tcgatcggca
     9841 ccacatcggc cgcgccatcg gtcgacccga agatcctggc cgacaggccg ctctcggagg
     9901 ccgacagcag gccgccgccc cagaccatgt gggcgatcgg gaagtaggcg agggtgaccc
     9961 acaggccgga gaagaccagc caggtggaga acttgacgcg gtcggcgatg gcgccgctga
    10021 tgagcgcgac ggtgatgatc gcgaacgtca gctggaagcc gacgaaggcc agctccggga
    10081 tattgccata gccctcatac ccgaacgggt tgaccaggac ggcggtgtcc ttcagcccca
    10141 acttgtcgaa gggattggcg aagatgccgc cgatatcgct ggggccgaac gacatcgagt
    10201 agccgaagag gacgtagagg acgcccacga gaccggcggc accgaaggac atcatcatca
    10261 tgttgaggac ggacttggcc cggttcatgc caccgtagaa gagggccaga ccaggcacgg
    10321 tcatcagaag gacgagtgag gcgcagatga tgacgaagcc ggtcgacgcc aggtcgatgg
    10381 tcggctcgtc ggctgcagcc agcgtgtgga gtgctgtcat gccggctacc caacgcccgc
    10441 ccggtttcgc cggatgcgtc gtcgtgttac gcgtatgttg cactctcccc catcgtggaa
    10501 acgtcgcgtt acatgccgcg cgaggtcccg gatgctgaga cgtcgtgtcc gccgacgacg
    10561 ggtgagagaa tgacgaggtg accacacgcc cgaccccggt cctcgtcttc gacggcgact
    10621 gcgccatctg ctccaccctg gcccggttca ccgcccggcg ggtgcgccgc cacgacgccg
    10681 acttcgcgat cgccccgtgg caggaactgg acctggcgtc gctcggcctc accgaagccc
    10741 agtgcatcga ggcgctgcag tggatcgggg ccgacggcaa gatctccgcc gcgcacaacg
    10801 ccgtcgccca ggccctctgg aacggccacg cctggttcaa gcccgccgcc gccgtgatct
    10861 gggcccccgg gatcaaccag ctcgccgcgg tcgtctaccg ctgggtcgcc gacaaccgcc
    10921 accgcctccc cggcggcacc cccgcctgct cgatcccccg ctaaagcaca acgctcgctg
    10981 cgctcgacgg tttccctcgg tcggcatact gctcgagcgg gaggaaggcg agcagcatca
    11041 ccaggtgggg ccagaaggcg atggtgatcg aggcataggt caccgcgtgg aaggcgaacc
    11101 aggcatagac gatgcggcgg cgccagcgct cggaggccag aaagatcagc ggagatgcca
    11161 tctcggcggc gaagagggcc cactgggtgg cctgcagcaa ccacggcgcg tcgaggagcc
    11221 acttcaccca gggtgtgccg cggcggacaa cggcccgggc catggtcgcc gagttgaccc
    11281 agtcccagcc gccgaagcgc actttcgccc acgccgagag gaagtaggtg gcgatggcga
    11341 agatctgcac cgcccgcagc acgaacccgc ccgcctggct gaggatgtcg cgcccgtcgg
    11401 ccgtccggcc ccaccgcgcc tgggcgaagg cgctgcccac ggtcggcagc aggaggaggg
    11461 cgatcacgaa gtcaccgcga tcgtggtcga ccttgccgta ggcataggcg atgtacatgt
    11521 accagatcca gcagatggcc acgacgctgc cggccaggcg gggcagccgg ccagtcgccg
    11581 cgacgagggc agccgcgacc gagccccact tgagcgtgtt gaccagcggg acggtcgcgg
    11641 cgggcaggtg caacagctgc cccattcgca agggttcgta ccagacgggg tcggcatagc
    11701 cgtggtacca gccgctggtc tgcagccaga ggacgtcgat gacgacgtac agatagacca
    11761 tggtgcgaaa gagcgccagg cgccgcagcg ggacggccgg gaagagccag ttcatgacgc
    11821 ccgccactcg gccaccaggt cgtcgcggtg ctggccggtc gggccgccgt cgcgcaacag
    11881 atagttgcgc acgacgacgc ggataccgac ccaggacggg tcgtcgggcc gcagggccgt
    11941 atggctgtcg gcaagggtct tgagcatcgc cgggtccttc gcgatgcggt cgaggttgcc
    12001 ttcgatctcc gcccggttca ggccgaccga atcggggctg atcggcgcct cgacccaggt
    12061 ggccggcgcg gccgccgtcc gcacttcgat gaacgtcgag cgcaccgcac cgttcgggtc
    12121 ggtggaggtg gagaacattc gccacggcga gaagggccac cagtcgtcat ccccgacgaa
    12181 ggtcccgccg aggaagagca ccgcggccag cagcacgacg gcgacgcgcc atgccttctg
    12241 cggcaaggtc agtggcacga tcaccggacc gtcgacctcc gcgggagccc cgccttactg
    12301 gaggatggca tccacgaatg cctcggggtc gaacggggcc aggtcgtccg gaccctcgcc
    12361 gagtccgacg agcttgaccg gcacgccgag cttgcgctgg acggcgacga cgatgccgcc
    12421 cttggcggtg ccgtcgagct tggtcaagac gatgccggtg atgccgacga cctgggagaa
    12481 gacctcggcc tggcgcagcc cgttctgccc ggtggtggcg tcgaggacga gcagcacctc
    12541 gtcgatgggc gactgcttct cgacgacgcg cttgatcttg ccgagctcgt ccatgaggtt
    12601 ggtcttgttg tgcaaccggc cggcggtgtc gagcagcacg acgtcggcct cgagttcgat
    12661 gccggccttg accgcatcga aggcgaccgc ggccgggtcg gcgccttcct tgtgcgagcg
    12721 caccgtcggc accccgacgc gggcgcccca cgtctcgagc tggtcggccg ccgcggcgcg
    12781 gaaggtgtcg gcggcgccga ggaggacgtc cttgtcctcg gcgacgagca cccgggccag
    12841 cttgccgacg gtcgtcgtct tgccggtgcc gttgacgccg acgacgagca cgaccgcggg
    12901 acgaccctcg acccggctgg aggcgactga gcgatccata ccgggatcga ccagcgcgag
    12961 caggtcggtg cgcaaccacg tgcgcacggt ggcctcgtcg gtcgtgccgt cgacctggac
    13021 ctgcgtgcgc agactctcga cgagttccat gctcgcctcg acgcccaggt cggcggcgag
    13081 gagggtgtcc tcgacctcct cccacgcggc ctcgtccagg ccgccgcggg tcagcagcgc
    13141 cagcaggccc ttgccgaggg cggtgttgct gcgggccagc cgggcgcgca ggcgctgcaa
    13201 gcgtccgcgg gccgtctcgg gccgttcgag cgtgggggtg gccggctcct cgaggaggac
    13261 gtcctcttcc ggctcgaccg gcggggcggg cagggtgtcg acgccgcccc ctgcgggttc
    13321 ctccgtgtcc gaggtcgccg gggggatggg cgtcgtcggc cgggtcggcg gcaccgccgg
    13381 tggccggcgg cgccgggagg gccggaggaa ggcgaacagt ccaccgacga tggcgaccag
    13441 caggacgccg gcgacaacga ggtactccca caactggtcg atcacaggcc catcctcaca
    13501 ctgcggagca ttggggcgtt aactctgtcc gctctcgaac tctgcccggt ctcggcctct
    13561 cgaactctgc ccgctctcgg gtccgtcgcc cggtatcggc ttcctcggct cctggccccc
    13621 gctcagcgct gcggggcgct ctccgtcgag gagtgcgggg cctcactcac cggcgtggcc
    13681 tcctcgccac ccgtcgggcg gatcctggcc gcggccgaag tcaccagccc ggtggtcttg
    13741 gtggccgctg cgtccgtgcc ggccttgacc ttgacgaaga cctcctcccc gcgcgccgtc
    13801 agcaggtccc gaccctgccg ccgcgccggg atcgccacca gcgccaggac gacgagggag
    13861 atcaggacgc acaggatcag gggaagaagg acgcggagca tggctccagg ttctcaaatg
    13921 tctacacgtg tcccaaatgc gacacgcaca acgcagcaac ggccgggtgg caagtgccac
    13981 ccggccgttg cgggaacccg ttagggctta tgtcacatgg tcacgccgtg gcgggccggg
    14041 acatagcgca cggaggcgct gtagaaggtg cgcgacgccc cgccaccggc gaaggtgaag
    14101 gtggccttgg tccgccacac gcccgggcgc gccgcgtggc tgtcggggaa ctggacgttg
    14161 ttgtagccga cgtacttggt ctgggtcgta gcgttgccac ggtcgtacca gtagccgttg
    14221 accagctgct ggatcgtgac gcgaccgcgg atgtaggccc cgccgccggc gcagtagatg
    14281 acgccgacct gccggctggt gttgccgacg ccgccgatga tgaacggctt gttggccttg
    14341 ccgtagcagg tgaccttggc gtcgctgacg gcaccggtgt cgggcgcggc gttggcggcg
    14401 ctggcgccac cgagagccat caggccggcg gacgcgacgg caacggcggc gcgagtcatg
    14461 gacttcacga ttgtttctcc cctccagcca ccgaccctgc gtcggcggca tgagcagaaa
    14521 ccttagaacg actccggggg cattggcagc ataacggacc ggtaacgctg tggcgggtta
    14581 ccgacagccc tggtgacttg ccgccaaagc cgtcgctgac ctgcagactt caggcgggct
    14641 cgtcgagcga acgtagccgc tgggacacga cattagtcac gccgtcaccg cgcatcgaca
    14701 cgccgtacag cgcgtcggcg acctccatcg tccgcttctg gtgggtgatc acgatgagtt
    14761 gcgaggagtc gcgcagctcc tcgaacagcg tgatcaaccg tcccaggttg gtgtcatcca
    14821 gggccgcctc gacctcgtcc atgatgtaga acgggctcgg ccgggccttg aagatcgcga
    14881 ccagcaaggc gacggcgacg agcgagcgtt ccccgccgga gagcagcgag agccgcttga
    14941 ccttcttgcc cggggggcgc gcctccacct cgatacccgt cgtgagcatg tccgacggat
    15001 cggtgaggaa gatccgcccc tcgccgcccg ggaagagccg ggcgaagacc cgctcgaact
    15061 gctcggccgt gtcgtggaag gcggccgtga agacctcctc cacccgctgg tcgacctcgc
    15121 ggacgatgtc gagcagatca cgcttcgagg ccttgagatc ctccatctgc ttcgtcagga
    15181 agctgtgccg ctcctccagc gccgcgaatt cctcgagcgc cagcgggttg atccgcccca
    15241 gctcacgcaa cccccgctcg gcccggcgca accgcttctc ctggacctca cggacatacg
    15301 gctgcggctc ccccggctcc tcgcccgctg ccacgacgac cggaaccagc tggtgcggcc
    15361 cgaagtcctc gacgaggacg tccgggtcga tgcccagctc ctcgatcgcc ttctgctgca
    15421 actgttccac gcgcgcctgc tgggcggccc gggccacctc gtcgcgatgg atgtcgtcgg
    15481 tgagtgcggc caggtcgtcc tgggcggcgc tcagctgcac ccgcagcgcg gccagctgcg
    15541 tctgctgttc tcgcgcccgc gcgtcgaggg ccgcgacggc accctccgcc cggccggtgg
    15601 cggcgctcac gcgcagcagg ccgtatgccg ccgccgcacc gaccgaatcg gccaccgccg
    15661 cctcgcgccg gcggcgttcg cggcgggctt tcagccgctc gcgggcggcg atctcgttgc
    15721 gggccgcgcc ttcgagggaa gcggcgcgcc ccgagagggc gcgggcacgt tcctcgcggg
    15781 tccgcagagc caacagggcc tcggtctcgc ccgcgcgggc ggcggtggcc gcggcctcga
    15841 gctcgccggt gcggtcgggg aagtcctcct cgctctcctc cggttcggcg ctcgccgcct
    15901 cgaaccgggc ggtcagctcg gcgagcgcgg cctggtcgtc ggccatcgcc gcggccgcct
    15961 gctcgcgggc gcgttcgagg cgctcgacct cggcccgcgc cgcccgggac gccgacccga
    16021 ggctgccgag ttgctcggcc acggcggcca tgcgggcatc ggattcgtgg aggcgttcca
    16081 ggtggccgtc ggcggcggcc gtggcctgct ccagcgcggt ccgggcgccg gcgacgtgaa
    16141 agcgcgcctg atccaatcgc gttgccgcgg aacgcagttc ggcctccgtc tcgtcgaccg
    16201 cggcctgggt ctcgatgagg ctgggggcgt cggcgctgcc gccccggacg tatgccgctg
    16261 tgacgacgtc accggcgggc gtcaccgcgg tcaggtcgcc cgattgctcg acgagggcgc
    16321 gggcagcggc gtcatccgcc accaggacga ccccgtcgag gagacgcgtg agggcgccgt
    16381 cgagggcgtc gggcacggtg accgaggcgc gggcccagcg ggcaccggcc ggcagcgcag
    16441 gggcagggtc ggccggcgcc ggcgctccct ggacgaggat ggtggcgcgc ccgccctcct
    16501 ggtcgcgcag atgcgcgacg gcagcactgg ccgcggtgag atcggcgacg gtgacggcct
    16561 cggcggcata ccccaacgcc gccgcgatgg cgacctcgtc acccggggcg acccgcagcg
    16621 actgcgcgag agtgccactc gtggcgacct cctcgctgcc gagcagggtc gcggcaccgt
    16681 ccttgcggcg caggcccagg cgcagcgcgt ccagccgagc ggtcagcgcg gtccgctccc
    16741 ggtcggcggt gcgctcctct tcgacccagc ggtcgaggtc ggcgcgggcc gcctcctgcc
    16801 gctgcgcggc tgcttcgtat gccgcgtcca ggccctcctc gccctcctcc tcgtcggcga
    16861 tcgagccttc gagggcggcg aattcctggt cggcggcgat ggcccgcgcg agcgaggcgg
    16921 cggcggcctc ggtcaggcgc ccgatctcgg cctcggcggc ctcgagccgg ctgcggcggg
    16981 cgccgacctg cccggcgagc ttggccagcc cctcgcggcg gtcggcgacg gcgcgggcgt
    17041 gggcggcgac gcgccgttgt tcggcggccg catcgccctc cgcggcctcg cgggcgtcgc
    17101 gggcgatgac gagcgcgtct ttcgcggccg cgacctcggc gagcaggtcg cgctcctgtt
    17161 cgcgggcctg ctgggcctgg gcgaggagtt cctccggatc ccggcccgtc gtcgtctcgt
    17221 cctcgtcgtc ggcgcgcagc aggcgcagcc gctcctcggc cagcgagtgc acgctctgca
    17281 ggcgttctgc ggtggtgcgc agggcgatcc agcgttcgcg ctcggcggcg agcaccggcc
    17341 cgcccgcggc gagttgttgc tcagagtcgg cgacggcggc ccgggcggtg gcgagcgcgc
    17401 tctcgacctc ggcccgtcgg cgcagcagct cggtctcgtc ggcggcttcc tgctccatcg
    17461 cggaggtgag ctgggcgagc tcgtcggcga gcaaccgcag tcgggcgtcg cgggcctcgg
    17521 cctggatgac ggcggccttg cgggcggcct cggcctgccg gccgaggggc ccgagctggc
    17581 ggcggatctc gctggtcagg tcggccaccc gggtgaggtt ggcctccatg gtctcgagct
    17641 tgcggagcgc cttctccttg cgcttgcggt gcttcaggac gccggcggcc tcctcgatga
    17701 acccgcggcg ctcctccggg gtggcgcgca gcacggtgtc gagctggccc tgcccgacga
    17761 tgacgtgcat ctcgcggccg atgccgctgt cggagagcag ttcctggatg tcgagcaggc
    17821 ggcagtgggt gccgttgatg gcgtattcgg agccgccgcc gcggaacatc gtgcggctga
    17881 tcgtcacctc ggtgtagtcg atcggcaggg caccgtcggt gttgtcgatg gtcatcgtga
    17941 cctcggcgcg cccgagcggc gcgcgcccgg aggtgccggc gaagatgacg tcctccatct
    18001 tgccgccgcg caggctcttg gcgccctgct cgcccatgac ccaggcgagc gcgtcgacga
    18061 cattggactt gcccgagcca ttgggcccga cgatgcaggt cacgcccggt tcgaggcgga
    18121 aggtcgtggc cgacgcgaag gacttgaagc ccttcagggt cagcgtcttg acatacaact
    18181 cggcggcgtc cttgcgtggt gggatcggcc cgcccactgt aaccgcggca tacccgcccc
    18241 gccggcaggc gcagcgtgag ctgtgccgat cggcccgtcg ctcagtcggt gctgtcggcg
    18301 gcgatcgcct cgtgatggcg gatcacctcg gcgatgatga agttcaggaa cttctccgcg
    18361 aactgcgggt ccaggcccgc gtcgtgggcg agggcgcgca gccgggcgat ctgccgctcc
    18421 tcacgggccg cgtccgccgg cggcagcccc tgggccgcct tgagttcccc gacctgttgg
    18481 gtggccttga accgctcggc cagcaggtgc accagcgccg cgtcgatatt gtcgatgctc
    18541 gatcgcagac ggatcagctc cgggggcatc cccgcgtcgg ttccgctcgg ctcgccccgc
    18601 gtcatacccg cgatggtatg aggcccgctc ccctccccgt gccccctgtc cagtccgcgg
    18661 cgggccggtc cgcggcgggc cggcataccg tgggcgcagc tcttgtagcc gaggtcagcg
    18721 ttcggcgaag ccctcgtagc cgcgtggggc cccgcgcacc ggcgtcgaga cggcccgcac
    18781 ggcacccggg cggcgcgtgg tggaggggta ttcgctgagc agggcgagca gccgcgccac
    18841 cgcgtcgggg gcgccttcgg cgcagacctc gacgcgcccg tcgtcggtgt tgcgggcata
    18901 gccgttcagg ccgagttcga gggcgcgggc ccgggtccac caccgaaaac ccaccccttg
    18961 cacacggccg ctgacgtgga cgacgacccg ctcggcatcc tcgggcgcgc tggggtcgtg
    19021 gtgatccggc attcggccag cttagggcgc gttcccgcgt gcctcccgca cgctgtcggt
    19081 gccggtccct acggtgtggc atggaggagg tgatctcgtc tcttccccgt gaccgacggc
    19141 gcgtgccgtc cgacctggag gtggccatga cggcgacggt ttcgccgccc ggctccgcga
    19201 ccgcgtcgtc gcggcccgcg ccgcgcgctc catcgcgggt ccgctccagc gcgccatcga
    19261 ccggtcgcct gcctggcctg gacgggctgc gcacggtcgc catcgtcgcg gtgctgatct
    19321 tccatctcaa cgccgcgtgg ctgcccggcg gcttcatcgg cgtcgacatc ttcttcgtcg
    19381 tctcgggctt tctcatcacg accctgctcg tgcgtgaacg ggccagcacc gggcgcgtcg
    19441 atctggccgg tttctggggc cggcgggcgc gccgcctgct cccggcgctg ctgctggcca
    19501 tcccggtcag cgtgctgctg gcgcgcctga ccgaggccga cctgctcgtc ggggtcggca
    19561 gtcaggtgct cggtgcgctc accttcgcgt tcaactgggt ccagatcgcc gcgggcgcgg
    19621 actacttcca cgcgacgacg ccgcagctgt tcatgaacta ttggtccctg gccgtcgagg
    19681 agcagttcta tctgatctgg cccctggcga ccctggtcct gctgagactg gtccgcagcg
    19741 gccgtgcgcg ggccggcatc gccggcggac tcgcggtgct ctccgccgtt ctcatggcgc
    19801 tccggttcga tcccgacgcc ggtgccaccc gcgtctacta cggcaccgac acccatgtct
    19861 tcggcctgat gatcggcgcc gcgctggcgt tctggtatgc cgacggtggg gccgcccggc
    19921 tggccgcgcc cgcctgggtg gcccgccggc gtccggtggc cgggatcgcc ggtgccgtgc
    19981 tgctgggcag tatgtggtgg ctcgaggagt cccgcgctct gaccttccgc ggcgggttgg
    20041 cgatcgcgag cctggccacc gcggcactca tcgcggtggc cgtcggcggc ggcacggtgg
    20101 cccgccttct ggacctgccc ccgatgcggt ggatcggcga gcgctcctac ggcatctatc
    20161 tgtggcattg gccggtcatc ttggtcgtca acgccgacat cacgtccgtg ccgggctccg
    20221 ccggattcct cagttcgcgc ctctgggcgg tcttggtcac cttcgccatt gccgatctct
    20281 cctaccgcct cgtcgaacgg cccgtgcgtc gcttcggctg gcgaggtgcg ttcacccggc
    20341 tcgccgcctg tgtcccgggg cgggccgacg gcgcgcgggt gctggccggg gcgatcacgg
    20401 tgctcgtcgt ggcgacggtg gccgtcatcg ccacggcgcc cgacgccacc tcgaccgaga
    20461 aactgatcgc ggccaacgcg aaggccgccg agcgcacggc cacctcgacc gccaccgcgc
    20521 ccgccctgac aacggcgacc ttcacgatgc ccaagggcaa ccagatcgac gcctacggcg
    20581 actccatgat gatcgggtcc gtgcccgcac tgaagtacta cttccccggc atccgcatcg
    20641 acgccaagtc caaccgccgc tggtcggccg ggctggccgc cgtgaccgcc tcgggcaccg
    20701 atatccgccg agccgtggtc ctgtcgttcg gcaccaatgc cggtgtcgac gaggccgatg
    20761 tccgagccac cttggacaaa ctcggcccca aacgcatggt cgtgctcgtc aacatccacc
    20821 tcaatgaggc gcgcgccacc gcggacaaca ccacgctcgc caaggtcgcg gccgactacc
    20881 ccaacgccat cgtcgccgac tggaacgccg aggtgaccaa gcaacgcggc ctgctccagc
    20941 ccgacggcat ccatccgagc atgaccggcc agcaccggta tgccgcgctc gtgcgccagg
    21001 ccctcgccga cctgtcctac cagcacaccg gcaaggtcgt gaagctcaag cccctcccca
    21061 tcccgtgacc cgcgtgccat gacgacaccg cccgccgcct gcgaggtcct caacctgccg
    21121 ctcgcggacc tagccgccgc gctgcgcacc ggctcgcatt cggtcgccga cgtgatcgcc
    21181 gcgtgcctgg agcggatcgc ccagcgcaac ggacccctga atgccgtcga ggcagtcctt
    21241 gccgaacccg tcctggccga cgccgcccgc ctcgatgccc tcgacccggc cgaccgcgcg
    21301 accctgccgc tgttcggtgt gcccgtcgcc atcaagtcgg agatcgacgt cgcgggcgtg
    21361 gtcacgacct tcggcgggca gggcaatacc cgcccggcgg ccgccgtgga acctcaccgg
    21421 ccacccggcg atcgtgctgc cgctcgggcg cacgagcgcc ggcatcccgc tcgccgtgca
    21481 ggtggtgggc cgcttcggcc gcgacctgga cctgatcgac gtcgcgaccc gcctcgaggc
    21541 gctcgtcacc gaggctccct cgcctcgcag tcagccccgg cgtggacgcg gctggcagcg
    21601 cgggcagctg aaggagctgc ggttcatgaa gtgctcgcgg cgcatcggcg tgtcgcagcg
    21661 gcggcacggc tggccacctc gcccataggc attcaaggat cgctcgaagt agcccgactc
    21721 gccgttgacg ttgacataga ggctgtcgaa gctcgtcccg ccctcgccga gggcgtcaga
    21781 catcacgtcg tgcgcgtgcc cgagcagccg cacgaccgcg ggtttggtca ggcggtcgcc
    21841 cggccgctcg ccgtgtagct tggcgcgcca gagcgcctcg tcggcataga tattgccgat
    21901 ccccgagacg atcgtctgat cgagcagctg ccgcttgatc gcgctggggc gcttggtgat
    21961 ccgtcgcgcg accgcgaggg ggtcgaagag cgggtcgaac gggtcgcggg cgatgtgggc
    22021 gacgggttcg ggtatgccgt cctggtccac cgcggtcagc gccagcccgc cgaaggtgcg
    22081 ctggtcgacg aaatgcacct cgatcgggtc gccctcaccg gtggtcacgc cgaagcgggc
    22141 gtgcaggtgg cgcgggtcgg ggtcgccggg gcgggccagg agcatctgcc cgctcatccc
    22201 gaggtggacc acgacggcct cgtcaccgac gtcgagccac aggtacttcc cgcggcgagc
    22261 cacctcgcgg atcgtgcgcc cgcgcaggcg ggcggcgaag tcggcggcgc cgggttcgtg
    22321 gcggcgcagc acccgggcgc cgtgggtctc gacgtcggtg atccgagcgc cggtgacgtg
    22381 cgcggcgagg ccgcggcgga ccacctcgac ttcggggagt tccggcaccg gggtcaggtc
    22441 gccgggtgcg gctgcggggt gaggacatcg cctgcgtggg cctggacagc ctgggcctgg
    22501 accgcctggg cctcgaccac gtgcccgtcg acggggacgg cctccggagc gtcgatcacg
    22561 gggccggcgg acagcgcccg ccaggcctgc tcggcggcct gctgctcggc gtccttcttg
    22621 gtgcgcccga cgccgacgcc gtgcacggtg ccgccgacga gagcggaggc ggtgaagacc
    22681 ttctcgtgtt cgggaccttc gccgacgatc gtgtactgga tgggtccgag gtcgcgggtg
    22741 acgctgagtt cctgcagcga ggtcttccag tccaggccgg caccgaggcg cgcggacgcg
    22801 gtgagcacgg ggtcgacgag atggtggacg aagacggtgg ccgcggccat accgcccgcg
    22861 aggtagacgg caccgatgac ggcctcggtg gtgtcggcga ggatcgaggt cttgtcgcgg
    22921 ccgccggtgg tctcttcgcc gcggccgagg aagaggaagt cgccgaggcc gatggtgcgc
    22981 ccgacgtcgg cgagcgcgcg catgttcacg gtggcggcac gcagcttggc caggtgcccc
    23041 tcggtgtaat cggggtaggt gcgatagagg tggtcggtga ccacgatccc gagcaccgag
    23101 tcgccgagga actccagccg ctcgttgtgt gggatggccc cgctctcata cgcgtaggag
    23161 cggtgggtca gcgccaacgt caagagcggc ccctcgacgg cgaccccgca gaccccaaga
    23221 atgtggtcgc ggagctcgtc gatgggccgc tgcatgaggg acggcctggg ttcaggcctg
    23281 gtgctcggtg cgctcggcgg cgccgtagtg gcgccccttg taggtgccgc aggacgggca
    23341 tgcgacgtgc ggcaccatcg gtgacttgca ctgcgggcag gtcgtggtgg cgaccgccgt
    23401 ggccttccag ttggcgcggc gcgaacgggt gttggagcgc gaggtcttcc gcttcgggac
    23461 agccacgtca gttccttttc tcagtagggt cggccgcgtc ggcgcccgtg gcgaggtccg
    23521 ccagggccga ccaccgcggg tcgagcagtt catgggcgtg cgccgggtcg tccgcgagac
    23581 ggaacccgca ttgcgggcac agccccgggc agtcgtcccg gcacaccggc tggaagggca
    23641 gtgctggcac caccgcatcc acgagcacgg gttccaggtc gaggtagtcg tcctggactt
    23701 cgtactcctg tgtttcctcg tccgtggcac ccaccttgcg gttgtgggcg gcccgctcgg
    23761 gatacgcgaa cagttcctgg aaggtggcgt cgacatcgac ggtcaccggc tccaagcacc
    23821 gcacgcatgc cccgaccgcc gtggcccgga ccgaaccgct cgccagcacg ccctcgagaa
    23881 ccgattccat ccgcaactcg acgtccagcg gcgcccccgc ggggacggcc aggacatcgg
    23941 tgccgatcgg ttccgaggac tcgatctcgc gcgtgacctg caccatcgac cccggacggc
    24001 ggcccagctc cacggtgctg aacaccatgg gtgagcgggg gtcgagacgg ttcatggctc
    24061 cacgcaagga ctgatggtcg gatcgggccg cgtcacgcga ggacacagac cgaaagtcaa
    24121 gcttaccggc gaggcccggc ggaccccaaa ttgccgttcg tcacgaggag cgcgagccgt
    24181 cccggcgggc caccagggcg tcgcggaccg ggtcgggcac gagcccggtg acatcgccgc
    24241 cgagcgcaca cacctccttg accagtgaac tcgagatgtg cgcgacggcc gggtcgcccg
    24301 gcaggaacag ggtctcgact cctgtgaggt gacggttcat ggaggccatc ggcacctcat
    24361 acgcgaaatc cacctcgccc cgcagcccct tcagcagcgc cgttgcctcg agatcccgac
    24421 acacgtcgac gatcagccgg ggcgtgaagg ccagcacccg caccccggac gtgccggccg
    24481 ttgcctcctc gatgaaggcg atccgttgct cgaccgggaa ggtgccgctc ttcgccgggt
    24541 tgtgcatgac cgcgaccacg acctcgtcga agagggcggc tgcgcggatg atgacatcga
    24601 gatgcccgtt cgtcaccggg tcgaaggagc cggggcacac cacgcgcgac gtcattgaaa
    24661 aaacctagca atcccgcgcc cggacaggca tacgatcggc ctccgtggca cgacggactc
    24721 tcgatctgct gaccggcgcc gcccgcgcgg acgagctcgc agcggtgctc gacgacctcg
    24781 tcgccgcggc gcggcaaatc ctcggtgacc gcttcgtcgg tgcctatctg cacggttccc
    24841 tcgcccaggg cgacgccgac ctggagagcg atgccgactt cctcatcatc accgacggcc
    24901 cggtcgatga ggaacagttc gtcgcgctcg cgcggctgca ccaccggctc gcgcacggcg
    24961 agagccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtga caccatcccg
    25081 gcccgagtca tcctgcacga gaacgggatt gccctggctg ggccgtcggc gaccaccgtc
    25141 gtgccgccgg tgccgggcga ccttgtcgtc gccgacgcgg ccgcacgatt ggctgaatgg
    25201 ctgcccgacc tggacgagtg ggcgttcacc gatgcgtgga gccagcgcta cagcgtgctg
    25261 aacctggccc ggatggtcgc ggcccacgag accgggcagg tcctgtccaa gcgcgcggga
    25321 gccggatggg cggcgacact gcggggcgga gagttcgccc cgctcctgga ggcaatggtc
    25381 gccgcccgcg gcgccacagc cttcgacgat cccgccgacc ccgcctggat cccccagcaa
    25441 cacgcgttct tcgcggccgt cgcggcgcga tccggcagct agggcgtggc tctcaattgg
    25501 cgacgcgtac gccacctacc ggtctgcggg cttctgccac tcaacgccgg gacggtgctc
    25561 gcaccggcca ctcgaccgca ggacggaccc gtgagcgcgg gagcagtcga ggcccgagac
    25621 ctcggttcgc gtcggcacca ttggccgacg agatgcccat gtcatggcaa tgggtcgcta
    25681 tccggcctcg gcgagccaga gtgtcgtctc gccgtagcgc cgggagccgg tggccgtcat
    25741 accgtcgggc cagcacggct cgggggagcg gctcgaacgc tccagcacca gcagcgcgcc
    25801 gggcgcgagc cagccggcgt cgacaagggc cgcgaggtcc gcggcgaggt cggcttcggc
    25861 gagggggtag ggcggatcgg cgagcaccag gtcggcgggc gcaacaacgg gacggcgggc
    25921 gaaggcgcgc accgtcatcg cgtggacgcg agccggtatg ccgaggtcgc gggcgttgcg
    25981 ctcgatcagc cgggcggtgg ggcggtcggc ttcgacgagg tcggccgagg ccgcgccccg
    26041 gctcaccgcc tccaacccga gggcgccgga cccggcatac aggtcgagca cgcgggctcc
    26101 cgcgatcacg tcgcgggcga cgagcatgga gaagagcgcc tcccgcaccc ggtccgaggt
    26161 gggccgggtc gcggctccgc gggggacggc gatggtccgg ccaccgaccc ggccgccgat
    26221 gattcgggtc atgtcttctc caggaagtcg gcctgctcgc ggcgcagcca ctcctcgatg
    26281 acgccggcga gttcgggatg gccgctgaga tcgggatcct gcgcgatcaa ttcggcggcg
    26341 tcggcgcgcg cggtggcgat ggtctcctcg tcgtgagcca gggagaggaa ctgcagccgg
    26401 ccccgtcccc cgctctgccg ggccccgagg atgtcgccct cgcgacgctg tcgcaggtcg
    26461 agctgggcga gttcgaagcc gtcggtggtc gccgcgacgg cggtcaggcg ctcgcctgcg
    26521 gggccgtcgg cagcccgggt ggccagcaag cacaggccgg ggtgtccgcc ccggccgacg
    26581 cggccgcgca actggtgcag ttgggagacc ccgaaccggt cggcgtcgcg caccaccatg
    26641 accgtggcat tggggacgtc caccccgact tcgatgaccg tcgtcgccac gagcacgtcg
    26701 agctcgcccg ccgcgaaggc ccgcatcacg gcgtccttct cctcggtcgg cagcttgccg
    26761 tgcaacaacc cgatgcgcag ccccgccagg ctggcctcgg tgctgagctc ttcgaaggtc
    26821 tccaggactc cgatcatctc cggcctcgcc ggagcctgct caccctggcc gaagccgtcg
    26881 ctgaaccagt cgacctcgcc ctcctcgtcg ccgcccgatt cgccgcccga ttcacccccc
    26941 gacgcgccgg gcccgatgcg cgggcacacg acgtaggcct gccggccggc gcggcactcc
    27001 tcaccgatcc gcgcccacgt gcgctcgagc catccgggcc ggtgttcgtc gacgacgaag
    27061 gtctggatcg ggctgcgtcc ggcaggcaat tcggtcagcg tggaggtctc catgtcgccg
    27121 aagaccgtca tggcgatggt ccgcgggatc ggcgtggcag tcatgaccag caggtgcggg
    27181 gtgccggatg ccttgccgcg caagacatct cgctgttcga ctccgaaccg gtgttgctcg
    27241 tccacgacga ccaggccgag gttgtagaag gcgacattct cctgcagcag cgcatgggtg
    27301 ccgatgacga tgccggcatc accggagacg acgtcagcca gggcccgccg ccgcgacgcc
    27361 gaaccctgcg acccggtcag cagcaccacg cgggtgccga tctcggaccc gccgagcaac
    27421 ccgccctcgg ccaggtcgcc gagcatcgcg gtgatcgagc ggtgatgctg ggtcgccagc
    27481 acctcggtcg gggcgagcag ggccgcctgc gccccgccgt cgacggcgct gagcatggcg
    27541 cgcaaggcga gcaccgtctt gccggacccg acctcgccct gcagcagccg gttcatcggc
    27601 acggtcgacg ccaggtcggc cgcgatctcg ccggccacct gctcttgacc tgcggtcagc
    27661 gtgaacggca gccggtcggt gaaggccgtg agcagcgggc cgccctcggc gatcgcgagc
    27721 gccgcctcct cctgggctcg ctggacccgc cgctgcgcca gcacggtctg caggaccagc
    27781 gcctcctcat accgcaggcg ctcgcgcgcc cgagcgacct cggcgcgggt ctcgggacga
    27841 tgcaacatcc gcaacgtctc gcccagcccg ggcaaccggt gccgcgaacg caacggcgcc
    27901 ggcagcgggt cgggcagcgg cccgagggca tcgaggacga gcccgacggc ctggacgagg
    27961 ttccagttgt gcagcttcgg gatcgccgga tagaccgggt agagccccgg cagcgccgcc
    28021 tccgccgcct cgtcgagcag ggtgtaaccg gggtgcgcca gctgccggcg atggttgtag
    28081 gtgccgacct ggccgccggc gatcacccgc acaccgggaa gcagcttgtt ctcgtgcgcg
    28141 cgggcgttga agaaggtcat gtcgacgtct tgatgcccgt cggtgaggac cgccgtcagc
    28201 atcgaaccct tgcgcgtcga catcgagcgc gtgtgcgcgc tcttgatcgt gccgatgagc
    28261 acgacgaact cgccgtcgcg cagttcggtg acctcgctgt catacgacaa gtagcgggtg
    28321 gggcgcatcc gcagcaggtc gccgacggtg acgacgccgc gggcgttgcg caggtgccgg
    28381 gcggcggagg cgatcagcgg cgccagattg gagtccaggt gcgccatcgc cccccctcac
    28441 ctcgccggtc gcctgtcgtg tgggacccta gccgagccgg ccgacaacct cgggcgccgg
    28501 gcgcgttttg cccgcgggga gggctctcgg ctaacctatg gaagttgcct gtatgccgcg
    28561 cgccgggcag ttgcctaaaa ctgttgtgcg ccgcgcacac ccgaaccact cacgaggaga
    28621 aacccccatg gctgccacct gcgatgtctg cggtaagggc cctggcttcg gtcactccat
    28681 ctcgcactcc caccgccgca ccaagcgtcg ctggaacccg aacattcagc gggtgcgcgc
    28741 gatggtcggc gaagccggca agaccccgaa gcgcctcaac gtctgcacct cctgcctgaa
    28801 ggccggcaag gtcagccgct gacccagaag caccacacgg aagcggccgg agcctcgtgc
    28861 tcgggccgct tttcggatgg ccgcgagacc ccttatcgcg ccgggtggaa gtggtcccag
    28921 cccgcctccg tcaggggtcg gccgtccagg tggacgcctg ggtcctcccc cgccgccggc
    28981 gcctccaccc gtcccaggat ccgccagtat tccggcacgc tcgcccggtc cgggaaggtc
    29041 gcgagcaggc tgtgctcctc accgccgttc agcacgcagg cgcgggcctg atccctcggc
    29101 accagcccct cggcccagtt caggtcgggt gccagcgtcg ccgagtcgat ctcgataagg
    29161 cagccgctcg cccgggcgat ccgggccgca tcgcgggaga gcccgtcgga gacatcgagc
    29221 atggcgctcg cgccggcgcg ggcggcgatc gggccctggc tcagcggggc gctcgggctg
    29281 cggtggtgcg cgagggcggc ctcctcacgg gcgtcgagcg cggtcccgga cgccgcccgt
    29341 tccagcagcg ccagcccgac cgccgagcgg ccgagcggac cggagatcgc cagcacgtcc
    29401 cccggctgcg cccccgaccg cagcaccggc tcggcgccgg ccagctcacc cagcgcggtg
    29461 atcgtcacca tcttgacccc cgcgggagcg gacgagagat cgcctccggc gaccgagacg
    29521 gcataccgcc gacactgctc tcccagcgcc cgcgcgaagt cgagcaccca cgccagcggc
    29581 gtctgcgggt ccagcaccag cgtcagcagc agcgtcgtcg gcaccgcacc catcgctgcg
    29641 atatcggcga ggttctgcac cactgccttg gccgcgacct gctccgcgtt cgaccagtcg
    29701 tcgcgccagt cccgaccgag gaccatcgtg tctgtcgtcg cgatcagcgg ggaggcgacg
    29761 tcgaggaccg ccgcatcgtc cccggggccg acgcgctcgg tgcgcgatcc ggcgagcagc
    29821 gggagcacct gggccagcaa ccggtcctcg gtgaggtcgg caacgacacc ctggggagac
    29881 gatgacacgt cccgcagggt agcgttctgc cggacatccc caatccgggg gcacccgatc
    29941 cttccgccgg ttcaccggcg ctacccgaca gggggacacc gtggttcagg cctacatcct
    30001 catccagacc gaggtcggca agtcagcgtc cgtcacggag gctgtctccg ggatctccgg
    30061 cgtcaccctc gccgaggacg tcaccgggcc ctacgactgc atcgcgcgtg tcgaggccgg
    30121 cacggtcgac gatctcggca aactcgtcat cgccaagatc caggaagtgc cgggcatcac
    30181 ccgcaccctg acctgcacgg ttgtccacgt ctgagccgaa ccctgggtat gccgcccgcc
    30241 cacccgagcg gtggggcgaa ctcctcctga cctcacccgg gcggtggggg ctggctgggc
    30301 tcgtcgccgc gccgttgctg ttggccgggt gctcccccgg cggcgtggac gtctcgccgg
    30361 cggcccaggc cgaggccgcg gcctgccagt ccgtcgcgtg gcccgaccgg ctcggcgaac
    30421 tcgcgcgcac ggcatccccg gacgacccgt ccctcgccgc ctggggtgac ccgccggtga
    30481 tcgcgcgctg cggcataccg gccctgacgc cgacgacgga cgagtgcatc gccgtcaacg
    30541 acgtcgactg ggtgctgcgc aagctcgacg acggctacgc cttcacgacc tacggcacgg
    30601 acccggggct cgaggtgctc gtgcccgacg actacgcgcc ggagacgatg gtcctggtcg
    30661 acttcaccga gatcgcgaag gggttgccgc gctcgggccg tgcctgcctc gccgtccgtg
    30721 ccgtcggggc gtccgtgccg tcgggggcgc cgtcggggtg aggccgccta gcgcagcccg
    30781 acggggcgct ccatcgccag ggcgatgagt tcgtcgatca gctccggata ggacaccccg
    30841 gaggcctgcc acatccgcgg atacatcgaa ctcggcgtga acccgggcat ggtgttgatc
    30901 tcgttgacga tcacatcgcc ggcgcgcgtg tagaaacagt cgacccgggc cagaccctca
    30961 cacccgagcg ccatgaaggc ccgcacggcg agttcgcgaa tccggtcgct gacctccggc
    31021 ggcacatcgg ccgggcagct gagttcgacc gcgtcggcgt cgaggtattt cgcgtcgaag
    31081 tcgtagaagt cgtgaccgtg accctcgcgc acgacgatct cgccgacctc ggaggtccgc
    31141 ggcggggcac cgtcatgtcc ttgcaggaca gcacattcga tctcgcgacc gtccatgccg
    31201 gcctcgacga tgaccttcag gtcgtggcgg cgggcctcct cgacggcggc gtccagctgg
    31261 tccatcgacg tgaccttgct gatgcccatc gagctgccgg cccggcacgg cttgacaaac
    31321 accggcatcc ccaggccggc gacggcgtcg cgggcgaggg cgggatcgtg gcgccattgg
    31381 gcgtcggtga tgaccgtatg cggtccgacc ggcagcccgg caccggcgaa gacgaccttc
    31441 atgaagtgct tgtccatccc ggccgccgag gcgaggacac cggatccgac gtagcggatg
    31501 tcggcgagtt cgagcaggcc ctggaccgtg ccgtcctcgc cgtaggggcc gtgcaacagg
    31561 gggaagacga cgtcgacctc gccgagttcg cggggcacct ctccggcgtc atgcacgctc
    31621 aggccgcggt ctcgggtgct cgtcggcacc agcacggtgg ccgcgtcctc gggcacctgc
    31681 ggcgtgtggt cgcgcgtcag ctccaacggc gtggggtcgt tgtcgagcag cacccacttc
    31741 ccgtccggcg caatgccgat cggcacgatc tcgtagcgct gcgggtcgat cgcgcgcatg
    31801 acgcccgctg cggtggcgca ggagacgacg tgctcggagg accggccgcc gaacaacagg
    31861 acgacacgga gctttccgct ggggtctgag gtgctggtca tcgcctccga cgatacctgc
    31921 gcgcggtgtc gcggaccggg cgcgcaacgc ccacgcaaca ccgggcgcct attgtcggcc
    31981 gtatgccagc cgcccgcgct ccgcgccgtc ccgccaccgc tgtcgttgcc gccgggcgtc
    32041 cgccggccga ggccggcgcc ggcctgaccg tgccgcccca cttcacctcg acctacacgg
    32101 ccggagggcc ggttgtatat gcgcgccagg gcaatccgac gtggtcggcg ttcgaggagg
    32161 tcgtcgcaac cttggagggc ggacgtgctc tggtcttcgc gtcgggtatg gcggcggtgg
    32221 acgccgcctt gtccgcgctg cctcatggcg ccgtcgtggt cgcccctgcc ggggcctaca
    32281 acggggtgga ctcggtgttg cgcgagggtg aagcggccgg gcgttggcag gtgcgttggg
    32341 tggatgtcac cgacaccgcg gcgatcgcgg gcgctgtcgc cggggccgac ctgctgtggc
    32401 tggagtcgcc gaccaatcct ctgctcgacg tcgccgacgt gccggccgcg atcgctgccg
    32461 ggcgggcggc cggtgctctg gtggtcgtgg acaacacctt cgccacgccg ctgctgcagc
    32521 ggccgctcga gtccggtgcc gacatcgtgc tgcattcagc gacgaagtac ctctccgggc
    32581 actccgacct gatcctcggt gtcctggtcg tcgccgtcga ggacgagcag cggtatgccg
    32641 cgctggcccg ccgccgcacg ctcggcggcg gcatacccgg gccgatggac gtctggctcg
    32701 ccacgcgtgg aatccgcact ctcagtgtgc gtttcgagcg gtcctgccgc aacgcggcga
    32761 tcctcgcggc ccggttggat cgccacgaag cgatcgagcg ggtgcggtat ccggggttcg
    32821 gggcgatggt gtccgtcgag atccgcgggg gcagcggcgc agccgacgcc gtgtgcgccg
    32881 cagcgtcatt gatcagcaat tccacgtcgc tcggcggggt cgagacgcag tgggaacgcc
    32941 ggcgggccat ccccaacgag cccgagacca cgccggtcaa cctcgtgcgg atcagcgtcg
    33001 gcatcgagga cgtcgaggac atctgggccg acgtcgagca ggccctcacc cgggcgggct
    33061 gacgcggccg gaccggcccg gcctgtgcac ccgctcggtg cacccgctcg gtaggttcag
    33121 ccggccttct tgtcggggtg cccgaattcg ctctcgccgt agtgcgccgg gtcgtagcgc
    33181 tcgacggggg cgacctgtcc gcgcaactgc tccacgccat tggtcaggga ggccatgatc
    33241 cggcgcgtcg cctcggcgac ggcggcccgg cggtcgggcg cgtcatacag atcggacagg
    33301 tcgaccggcg gttgcgcgat cacacccatc gtcttgcgcg gcaacagctt cgggagcttc
    33361 gtgtagggcg ccagcaactc gttcggaccc cacatcgcca cgggaatgac cgggcagcgg
    33421 gtctccagag cgatccgggc cgcgccggtc ttgccgcgca tcggccacag gtccgggtcg
    33481 cgtgtcaacg tgccctcggg atagacgccg acggccttgc ccgcgcgcac cgcggcgacg
    33541 gccgagcggt aggcatcggc gacgcgatgc gtgccgcggt gcaccgggat ctgaccggcc
    33601 ctcgtgacga tccagccgac gaccggcacg cggaagacac tctccttgcc gaggaagtac
    33661 accggccggc ctgcgtcata catgaagtgg cccaagatga gaacgtcgat gtgcgacagg
    33721 tgattcggac acacgacgaa accgccctcg gcggggatat tggccacgcc tcgccagtcc
    33781 cgtttcgtca ggacctgcag gaccggacgc agcacccgga cgacgaaacg gtaggcgccg
    33841 gggaggcgcc gctggtcggg agtgcggatc acccgtgcag cgtatccgcg ccgtggcagg
    33901 attcctggca atggacgagg acctggagtg cgtcgtcatc gtgccggtcc agggcgcgcc
    33961 gcacggcaag agccggctta cggtgcccgg ccgcgccggc ctcagccgcg ccttcgccgc
    34021 cgacaccatc gacgcggtca tgcgggtgcc cagcgcgcat ctggtcgtcg tcactcccga
    34081 tcccgtcctc acccgatatg cccgcgccgc aggggcactc gtcgtcctgg acaccggagc
    34141 cggtctcaac gccgccgttg cggccgggat ggcggcaggc gtcccgttcg gcgcactgcc
    34201 catggcggcg atgctcggcg acctgccggc ggtcacgccc gaagcggtgc gttccgggct
    34261 gatgcagtgc gccggccacg agcgggcgtt cgtccccgat ctcgcgggca ccggcacggt
    34321 tttgctgtgg tcgcgtgacg gacggctgca gccggccttc ggaccggggt cggccgccgc
    34381 tcacgaagcc ctcgggtatg tccggtgcga gcgggtcccg tcgcacctgc gcctcgacgt
    34441 cgacgtccgc gcggatctcg ccgtcgccat cgagctgggg tgcgggcggc atacggctgc
    34501 cgccctcgcg gcgggcgcgc tgtcgcaccc cgtctcgcac gagggcctag ggtgagtgcc
    34561 atgcaggcga gcgtgcaccg gtacgacgcg gccgatgcct cgggcgccgt catcctcgat
    34621 aacggggtgg tcctcccctt cacgtcggat gccttgcgcg acagcggtct tcgcggcctg
    34681 cgcgtcgggc agcggctgag catggaggtc acggacgccg atccgccgac cgtcacgcgg
    34741 ctgtggatcg tcggcatcgg cgagggccag agcatcggct gaccgcaccg cagagcccgc
    34801 gacaccacac cacgccccga cagcacaaaa cccccggctc accggagcga gccaggggtt
    34861 gtgcggaaca gatcagcggg cgctcttgcg cgcgggcgcc ttcttggccg gggccttcgt
    34921 cgcggcggcc ttcgtcgtcg ccgtcttctt ggcggcggcc ttcgtcgcgg tcttggcggc
    34981 ggccttggcc ggagcggcct tcgtcgcggc ggcgttggcc ggcgcagcct tacgggccgt
    35041 cgacttcgcc ggcgcggcgg cggtcttggc ggcggcgctc gaggcgcgcg cgagcggcgc
    35101 ggacttgggg agggacttcg ggttcttgac gtagtccttg aacttcgcgc tggcggtgaa
    35161 gcgcgggacg gaggtcttgc ggatcttgac cgtctcgccg gtgcgcgggt tgcggccggt
    35221 tcgcgctgcg cgagcggcct tttcgaaggt gccgaagccg gtcagggcaa ccttgccgcc
    35281 cttggaaacc tcggcgacga tcgtttcgag aatggtctcg accgcgtcag tggcggcctt
    35341 gcggctgccg agcttgccct cgatcgcctg aaccagatct gccttgttca cgagtctttc
    35401 tccatcgtgt caatgccgga ggggccggcg acccaacgag accgccgctg ttcgaaaaac
    35461 ggtctcgctg ttgacggtag gactcaatgg cccgttagtc cagaatcacc gcacggtgag
    35521 tttcgccgtg tcgcaacgga ttagcgtcaa atcccgctct cagccagggg ccacgacggg
    35581 cttgaacgcc ggccgattcc cctcgtacgc ggtgatatcc ggctcgtggc gcagcgtcag
    35641 gccgatgtcg tcgagaccct cgaggaggcg ccagcgggtg taatcgtcga cctcgaagcc
    35701 ggccacgatg tctccggcgg tgatggtgcg cgacgccaaa tcgactgtga cagaagcacc
    35761 tggctcgttc tcgaggtact tccagagcaa ctcgacatcg tcctgactca cgacggcggt
    35821 gagcaggccc tgcttgccgg agttgccgcg gaagatgtcg gcgaagcggc tggagatgac
    35881 gacgcggaag ccgtagttca tcagcgccca caccgcatgc tcgcgcgagg agccggtgcc
    35941 gaagtcgggg ccggcgacga gaaccgagcc gccggcgtag tcggcgttgt tgagaatgaa
    36001 ggagtcgtca tggcgccagg cggcgaacag cccgtcctcg aaaccggact tggtaacccg
    36061 cttgagatat tcggccggga tgatctggtc ggtgtcgaca ttgctgcggc gcaacggaac
    36121 cccgatgcca gtgtggctgg tgaaggcctc catgtcagtg ttcctctccg gtggcagtgg
    36181 cgagcggcgc gaggtcggcg gggctggaca gcgttccgcg gacggcggtg gcggcggcga
    36241 cgaggggact gacgagatgg gtgcgcccgc ccttgccctg ccggccttcg aagttgcggt
    36301 tggacgtgga ggcactgcgc tcctgcggtg ccagcttgtc gggattcatc gcgaggcaca
    36361 tcgagcagcc cggcaaccgc cattcggcgc ccgcggcctc gaagacctct ccgagtccct
    36421 cggcctcggc ctccagccgg acgcgagcgg accccgggac gacgagcatc cgcacgccgt
    36481 cggccacctt gcggcccttg aggacggcgg ctgcggcgcg caggtcctcg atgcggccgt
    36541 tggtgcacga gccgacgaag acggtgtcaa cggcgatctc ccgcaacggt gttcccgctg
    36601 tcaggcccat ataggcgagt gcggcctcgg cggcgcgggc ctcggtctcg tcggggatca
    36661 gcgcggggtc gggcacgctc gccccgagcg gcgcgccctg cccggggttg gtgccccacg
    36721 tgacgaaggg ggtcaaggta gccgcgtcga tgtcgacgac cgcgtcgaag acggcatcgt
    36781 cgtgggtgcg caacgtctcc cagtagccga ccgcggcctc ccactccgcg cccgtcggcg
    36841 cgtggtcgcg gccctgcaga tagtcgtatg tcgtccggtc cggggcgatg agaccggcgc
    36901 gggcaccggc ctcgatcgac atattgcaca ccgtcattcg cgcctccatg gagagattgc
    36961 gaatcgcctc gccgcggtat tcgaggacat agccctggcc gccgccggtg ccgatcttgg
    37021 cgatgacggc gaggatgatg tccttggcgg tgaccccgtc ggcgaggtcg ccgtcgacat
    37081 tgacggccat cgtcttgaac ggtttcagcg gcagcgtctg ggtggcgagc acgtgctcga
    37141 cctcggaggt gccgatgccg aaggcgaggg ccccgaatgc gccgtgggtg gaggtatggc
    37201 tgtcaccgca gacgatggtc atcccgggct gggtcaggcc gagctgcggc ccgacgacgt
    37261 ggacgatccc ctgctcggcg tcgcccatcg ggtagatcgt gacgccgaac tcctcgcagt
    37321 tgcggcgcaa ggtctccacc tgcgtcaggc tgaccgggtc ggtgatcgga cccggcgtgg
    37381 tggggacgtt gtgatcctcg gtcgcgaggg tgaggtccgg gcgccggacg ggcctgccgg
    37441 cgagccggag cccgtcgaag gcctggggcg aggtgacctc gtggatcagg tgcaggtcga
    37501 tgtagagaat gtcgggttcg ccctcggcgc tgcggacgac gtggtccgcc cagatcttct
    37561 ctgccagtgt tcctgccatc attgcctcca acgccacgtc cgacggcccc tggtgggcgc
    37621 ggattcgggt cacgtgctgc gatcttcctc gaaggtttcc acgcgcgcga aggcccagga
    37681 cttgcgtctc aggcaatgag acggcaatat caatgcatgg acaactcgag tggggtcggc
    37741 gtcctcgaca aggccgctgt cgttttgggt gctctggagt ccggcccggc gaccctggcc
    37801 cagttggtga cggcgaccgg attggcccgc ccgacggcgc atcgtctggc cgtcgcgctg
    37861 gagcaccacc gcctcgtcgc gcgtgacctg caggggcgtt tcgtgctcgg cccgcgcctg
    37921 ggcgaactgg ccgcctcggc cggtgaggac cggctgctcg cggcggccgg cccggtcctc
    37981 ggcgccctgc gcgatcacac caacgagtcc gcccagctct tccgccgcca gggcgattac
    38041 cgcgtctgcg tctcggccgc ggagcgaccc gtcgggctgc gtgactccat acccgtcggg
    38101 gcgaccttgt cgatgcacgc cggctcggcc gcccaggtgc tgctcgcctg ggaggagccg
    38161 gaccgactac accgcggcct gcacggggcg gccttcaatg ccacgaccct ctccggcgtg
    38221 cgtcgccggg gctgggccca gagcgtcggc gagcgcgaac ccggcgtcgc gtcggtctcg
    38281 gcgcccgtgc gatccccctc cggccgcgtg atcgccgccg tgtcgatctc cggccccatc
    38341 gagcgtctct cccgccagcc cggccgcctg cacgcggcca ccgtcatcgc cggcgccaac
    38401 aagctcaccg aggtcctcgc ccggcacgcc gccgcccagg aggccgccgc ggccagggcc
    38461 gccgcgcact gagcccccga atacgccccc tccgcgagag cgccggaggc gcagaaaggc
    38521 cccctgtcgt gaggacaggg ggcctttctc tcgcgtagcc ccgacggggt tcgaacccgc
    38581 gctaccgcct tgagagggcg gcgtgctaga ccgctacaca acggggccgt gctacagcgg
    38641 agcaaccata cccgggtgga taggcaatct cccaatcagc gcgaggcgcc gaactgcgct
    38701 ggggtaccag gactcgaacc tagaatgagt gaaccagaat cactagtgtt gc
//
LOCUS       DAOPJG010000010        37359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1172_length_37359_cov_8.272947, whole genome shotgun sequence.
ACCESSION   DAOPJG010000010 DAOPJG010000000
VERSION     DAOPJG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 37359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37359
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1172_length_37359_cov_8.272947"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PKU75_02125"
     CDS             complement(<1..1093)
                     /locus_tag="PKU75_02125"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HPF79701.1"
                     /translation="MRIVVPKETRPGESRVAATPRTVEQLIKLGYDVAVETGAGRASA
                     YEDSAYTAAGAAIVPPEQAYAGDIVFKINAPDAEEIERLRSGQILMSLMAPALNPQLV
                     EALRAKGVTAMAMDAVPRISRAQALDVLSSMANLGGYRAIIEAANEYGGMFTGQVTAA
                     GKMPPATVLVVGAGVAGLAAIGTANSLGAVVKAFDARQEVADQVESMGGQFLRPNLEG
                     LDTGPSADGYAKEMGEEFNKRTTEMYREEVPKADIIVTTALIPGRPAPRIIDEAMVAS
                     MKPGSVIVDMAASNGGNVAGAQADQVVTTANGVKILGYTDLPGRLPTQASQLFGQNLV
                     NLMKLVTPEKDGQAVLDMDDTVVRGMTVAQ"
     gene            complement(1228..1614)
                     /locus_tag="PKU75_02130"
     CDS             complement(1228..1614)
                     /locus_tag="PKU75_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79702.1"
                     /translation="MTLAVRMLEGREPYDIYTWAQELFEGREYIEAGQVLEYLLEHHG
                     SEPGLGEARELLARSYFHSAQLMRAVDTAHEILQGDPGNSYALLILVRSLQRAGRTDE
                     ALAAERLAAAYGVDVSRPGGSAAETG"
     gene            complement(1690..2352)
                     /locus_tag="PKU75_02135"
     CDS             complement(1690..2352)
                     /locus_tag="PKU75_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPF79703.1"
                     /translation="MPELRRDDSLWILDFGSDENRFSPDWLDAVNGHLDELEAATEPG
                     ALVTTATGKFFSNGLDLDWVQAHPDQFFPYVKRVEEFLSRVLTLPVPTVCAINGHAFG
                     GGALIAFSHDYRIMRGDRGFFCLPEADIRIPFSVGMSALVQSKLTPRSAIDAMTTARR
                     YGGAEALSAGIVDAVASEEEVLPTAIELARSLVGKDRGTLGTIKQRMFASVTAALAEP
                     LA"
     gene            2386..3204
                     /locus_tag="PKU75_02140"
     CDS             2386..3204
                     /locus_tag="PKU75_02140"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPF79704.1"
                     /translation="MTGSHPLDVLFDAAAAGSEVEIPEGWGQGRAVYGGLVAGTLQAH
                     ALGAFDLEPDALRATTTSFVAPTECGPARLEAVLLRRGSSATQTEVKLWQRDSRSGEE
                     TVRSVQLSAYGADRESSVDLAPAPPRHTIPDPDPLMVVPVVPGITPEFFAKVQLRPAH
                     GRFPFSGSTTGDMAGFMRLTETPRALALPHFIALVDAWPPAPGQLLRSPAAMSTLTWT
                     LEMLTLPTGPADQHWWYEVDTDAAHDGYAHTHAHVFTRDGAPVAISRQTITIFG"
     gene            3231..3404
                     /locus_tag="PKU75_02145"
     CDS             3231..3404
                     /locus_tag="PKU75_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79705.1"
                     /translation="MNSERRRHTVAALLAAGLWGTTGTVAHHAPAGSNQALVGLATFD
                     ITAAERRATAPTP"
     gene            3418..4395
                     /locus_tag="PKU75_02150"
     CDS             3418..4395
                     /locus_tag="PKU75_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79706.1"
                     /translation="MTPEVVAAARVPWTSPAIRSWYSAPSLLAQLADGVRQEVAWVGD
                     EEFGAELRGHVDSASPMPPLAWANRVLDLPDGGWALCGIRFRQRDNARPFVDVVASTA
                     PPTPDGLATIAAAVAPAYDPWHPLALRANLPDPDAIIAAVRGDSRFVGMSVDMYVMAG
                     LVHRLRTRKRDIDDRLRLVPGSPGELAARNAGIYGELYRRDPERARWATPEDADSLAD
                     CADEGLLFEVRVHDEPAGVVAAMRWDAHGMCGFSVEELALDAEHREKGLGPVVLQRLL
                     RKLPADDGDALWGTIHHNNIPSLRNALRVGREIVGANMWITPAGWSGMP"
     gene            complement(4392..4739)
                     /locus_tag="PKU75_02155"
     CDS             complement(4392..4739)
                     /locus_tag="PKU75_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPF79707.1"
                     /translation="MTNSAPAGAQPAQTCCALTKIPVSAADAERLAPMFKALGDPVRL
                     RMASLIATQPEVCVCEITPAFDLSSGTISHHLKSLRDAGLVDSERRGTFVYYWIRPEA
                     LAALSALLSVDCC"
     gene            4819..5700
                     /gene="arsM"
                     /locus_tag="PKU75_02160"
     CDS             4819..5700
                     /gene="arsM"
                     /locus_tag="PKU75_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HPF79708.1"
                     /translation="MSSNLTDDQVRDAVRERYAASAHLSLSGKVDSCCGGTVPAPGES
                     SCCGEAGKARGLDPITGGLYDETDVPFDAALAASLGCGNPTALAELAPGEDVLDLGSG
                     GGLDVLLSARRVGPEGTAYGLDMTHEMLALAERHKTEAGIENAQFLLGTIEQVPLPDA
                     SVDVVISNCVINLSPDKDAVLREAFRVLRPGGRFAVSDIVLLREIPQELRGVLALWTG
                     CISGSLRDEEYVAKLAAAGFHDAGVEVTRAYSRADLEDLADQMDLTALPEGMDPAGAI
                     DALDGAFASAFIRATKP"
     gene            complement(5708..5842)
                     /locus_tag="PKU75_02165"
     CDS             complement(5708..5842)
                     /locus_tag="PKU75_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79709.1"
                     /translation="MSDKSQKLVMQWRPVTGRDGRTRMEARWVTPAADRHPAQDRRAA
                     "
     gene            5955..6923
                     /locus_tag="PKU75_02170"
     CDS             5955..6923
                     /locus_tag="PKU75_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79710.1"
                     /translation="MPPTAATTRRIVRSPAREACSLLPHEPGVYRFRDERDRVMYVGR
                     ASDLRSRTRSYWGDLHGRRHLRHMVGQIARVEALVCQSVHEAAWLERNLLTRSLARWN
                     RTRGGQEVPVWFVLDPGPRAPSLSLSYGPPPDPELGFGPYLGSTRARLARSGALRAWP
                     VHLTGTHLDAGTRSIGEARGVDPSQRAAVIAGLVGLLRGVEDDVAATRARLVESRSRA
                     AAQLGYEVAGQIQEELAALEWSVAPQTVAGCTPPDLRVAAWHDGLLLTLRARDGFVDV
                     WRTRDCPARQGAQLIAATPPGWQQFADRNVRLLVALRDAQAGANRA"
     gene            complement(6956..7591)
                     /locus_tag="PKU75_02175"
     CDS             complement(6956..7591)
                     /locus_tag="PKU75_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPF79711.1"
                     /translation="MSADSQPAPTIESGELRRAVTLVAALNFAYFFVEFAVAQVAGSV
                     SLLADSVDFLEDTAVNLLILIALGWPLARRAVAGKAMAVVVLVPAVVACWEAIQRFTD
                     PAAPDPPPIVLASLGAIVVNGTSAWLLARVRHHGGSLSKGAFLSARNDVLVNLAIIAM
                     AVVTVWNATGWPDLILGVFIIALALRAAHEVWEVSEEERLAAKALAGEDLD"
     gene            complement(7688..9082)
                     /locus_tag="PKU75_02180"
     CDS             complement(7688..9082)
                     /locus_tag="PKU75_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79712.1"
                     /translation="MSTAPTRTGGEWLQAWDPEDEARWDKGLAWRTLWITTFCLTLAF
                     IAWFLPSAIIPKLNSLGYSWSKNQLYWMAAMPGLAAGLFRLVWMVLPPIMGTRKMVSL
                     STLLLIASTLGWGVRVQHPTAPYWELMVLAFLAGIGGGLFSGFMPSTSFFFPRRMQGT
                     ALGLQAGLGNFGVSVVQLLTPYLIAMSGLAIFGGSQVLKTAGKPDKNVYFQNAAFVWI
                     PLMIIGAVLAWTMLKSVPVKANIRQQFDIFSNQDTWWMTLIYIMTFGTFSGLAAQFGL
                     LMANLYGSGNDKVVSGTGAAAKVLIEGYAVPDPVKYVFLGPLIGAGARVLFSPLTDKM
                     GGAIWSLISGIGLIASIIYTIPALTPDFSSAAALKGDFNHFLWGMLAIFLFSGIGNAS
                     TFKQMPMIFERRQAGGVIGWTAAIAAFGPFFFGVGVSSLGPTAFYWIGVAWAIMCTII
                     VWMRYARPGAPKPS"
     gene            complement(9161..10525)
                     /locus_tag="PKU75_02185"
     CDS             complement(9161..10525)
                     /locus_tag="PKU75_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HPF79713.1"
                     /translation="MARPDAPTGATPVDADTPERHAGASRVLWMSTAAFTLMFAVWLM
                     FGILGVPIQKELKLTDPQLAWLSAVAVLNGSIWRLPAGMLADRIGGRKVTIAMLLLTA
                     IPAFLLTRAHGYTQLLILAFLVGFAGNLFSVGIAWNSAWTPKQHQGFALGVFGAGNVG
                     ASVTKLLLVLFPAIIAGTVGHTYFGIFEGGWRIFPAIYCVALVTMALLVYLVVPRQDR
                     VAGAGKPIGEMLAPLGHIRVWRFSLYYAAVFGAYVALSAWLPKYYVDNFDIELKTAAL
                     LTAIFIFPASLLRPVGGWFSDRFGARRVMYWTFGVMLLVTGILMMPNGHIVIAHADGS
                     QSDHLGYHISLPFFVALVFLLGCAMGIGKAAVYKHIPEYFPDNVGSVGGLVGMFGGLG
                     GFVLPPLFAYTKQWSGFPTSTFFVLFLLTAVCAVWMHWTVVHMLHKENPELARHLEAP
                     AATR"
     gene            10701..11513
                     /locus_tag="PKU75_02190"
     CDS             10701..11513
                     /locus_tag="PKU75_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF79714.1"
                     /translation="MSDPALAAALLSSPVRRQIVDAIAGAQAARVPGTGAPALGAEGL
                     SAAQVAKAVDLHVTTARFHLDQLVAAGVLVASFHKHPGAGRPRKVYAVTRRPVSAENP
                     EVPTGLLMEVLAGTLSAGRDEGRVVEPDEAGRRWALENVPAHDASPAHSAGEFVGKVG
                     GLIDVLQHWGYEPELSTRDQGRSVQIDLPHCPFRGLARRHTDVVCGIHRGLIAGTMDR
                     LGEPNTTVTLLPFAEGGTCIAHISRTTPFDPAEPQPRPTDPDRKHTRKHADD"
     gene            11506..15192
                     /locus_tag="PKU75_02195"
     CDS             11506..15192
                     /locus_tag="PKU75_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HPF79715.1"
                     /translation="MTDTGFDSPLADALVGTRRFFTKAQVSDDKRSMYVAGGRSGDSF
                     YRDRWSHDKVVRSTHGVNCTGSCSWKVYVKDGIITWEAQQTDYPSAGADKPEYEPRGC
                     PRGASFSWYTYSPTRIRYPYVRGVLLEMYRAAKQAVGGDPVVAWASIVQDPDKAARYK
                     SARGKGGLVRSSWEEVVELVAAAHVYTVKRWGPDRIAGFSPIPAMSPVSYASGARFLE
                     LVGAPMLSFYDWYADLPNASPQMFGDQTDVPESGDWWDAGYLMMWGSNVPLTRTPDAH
                     WMTEARYRGQKVIAVSPDYAENVKFADEWVACNPGADGALAMAMGHVLLKEFFADSQV
                     DFFVDYNRKFTDLPMLVTLEEGENGWEQGKYVVAGDLDVPEKDSENAMWKTAVLDQRT
                     GQVAIPNGSVGYRYGEEGWGKWNLDLGDIEPALSVYDTRDDAVLVRFPRFDADGGSVV
                     FETRGVPVKRIGDRVVTTVLDLMLAHYNIAREGLPGDWATSYDDADAVNTPAWAERIT
                     AVPASQIIRLAREWTHNAIDTGGRGMILFGAGINHWFHADQIYRAMLVLTTITGCQGR
                     NGGGWAHYVGQEKIRPIMGFQHMAFALDWHRPPRHMNQTAYWYVNTSQYRYDTFTADD
                     LDMGTGVFAGKGVMDLLALSVRLGWTPSYPTFNRSSLALADEAKAAGMEPVPYVVEQL
                     KSGELKFAVEDPEAEENFPRILSLWRSNLFGSSAKGNEYFLKHLLGTDNAVTASQAPA
                     DKRPDGIVWPEQVPEGKLDLLTTLDFRMTSSTLFSDVVLPAATWYEKHDLSTTDMHPF
                     VHSFNPAIAPPWQSKSDWDSWKEIAKKFSELAVDHLGTRTDVVAKPLWHDTPEALATV
                     HGSVRDWRTGEVEPVPGKTLPVLAVAERDYTAVYDKMTSVGPLMEKVGMLTKGVPYDV
                     QRETEILRQRNGTQRGGAGDGQPKLDTAIHVADAILHFSGTANGHLATLGFKNVEKRT
                     GTQLHDLSAEHEGKQITFADTQIQPVPVITSPEWSGSESGGRRYAPFTINIERLKPFH
                     TVSGRQQFYLDHDWMQGMGETLPVYRPPLNMTMLFGETPLGEKTELGVSVRYLTPHNK
                     WSIHSEYQDNLFMLSLSRGGQTIWMSDRDADKVGIKDNDWVEAVNRNGVVAARAIVSH
                     RMPEGTVYMHHAQDRLIDVPLTETNGRRGGIHNSLTRIMIKPSHIIGGYGQFAYFFNY
                     IGPTGNNRDEVTMIRKRTAPVRYN"
     gene            15236..16906
                     /gene="narH"
                     /locus_tag="PKU75_02200"
     CDS             15236..16906
                     /gene="narH"
                     /locus_tag="PKU75_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HPF79716.1"
                     /translation="MRVMAQLAMVMNLDKCIGCHTCSVTCKQAWTNRTGMEYVWFNNV
                     ETRPGLGYPRRWQDQAEWEGGWVRKANGRLTLAAGGRMKKLLNIFSNPKLPSIDDYYE
                     PWTYDYETLLNSPQQQQFPVARPLSLISGKPMNVTWSANWDDDLGGAREYGPKDPMIK
                     GIEDKVKFEFEQTFMFYLPRICEHCLNPACAASCPSGAIYKRSEDGIVLVDQERCRGW
                     RMCISGCPYKKVYFNHKTGKAEKCTFCYPRVEVGIPTVCSETCVGRLRYIGLMLYDAD
                     AVLDAASVENDQDLFAAQRGVFLDPEDPAVQREAERAGIPGDWIEAARKSPVKRLIMD
                     YQVALPLHPEYRTMPMVWYIPPLSPVVDVVKETGHDAESQANLFAAIDTLRIPVEYLA
                     NLFTAGDVAPVNEVLKKLAAMRSYMRDINLGRDGNADIPATVGMGEEEMYEMFRLLAI
                     AKYADRYVIPTAHAEQAHALEELATDCAVSEYGGGQSAAFGEGSGRGGLTPVAVENLR
                     VLKETQTADSLEELPGENPLRGRVNLLNWDGKGMPDGIFPGDKTGTPQ"
     gene            16903..17616
                     /gene="narJ"
                     /locus_tag="PKU75_02205"
     CDS             16903..17616
                     /gene="narJ"
                     /locus_tag="PKU75_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HPF79717.1"
                     /translation="MIWRRHRKSAPALSRTDIRTLWQVTSLLLDYPTEQLRAQAPTLR
                     AALAQVPADARAGLTAYLDHLQATSLASLQSDYIDTFDITRKCALHLSYYTCGDTRKR
                     GVELIRFKQAYRAAGVEMSGDELPDHLCVVLEFGAVHDVDIAWKLLCDNRVGIELLHR
                     ALRAKDSAWLPVVQSLRATLPELDGSDEEALAKLIAQGPPSEQVGLDLPGYAMDPRLN
                     PRPLPIDLGDAIPVGAQQR"
     gene            17675..18418
                     /gene="narI"
                     /locus_tag="PKU75_02210"
     CDS             17675..18418
                     /gene="narI"
                     /locus_tag="PKU75_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HPF79718.1"
                     /translation="MNRFFFGIFPYLCLAVFVVGHIWRYRYDKFGWTTRSSQLYEDKL
                     LRIGSPLFHFGMLGVVGGHILGLLIPQSWTDKIGLSRHAYHVLALAGGIPAGVAAVVG
                     LIILVYRRRTTGPVFSATTVNDKVMYVVLGAVIVLGIWNTIAGSWLQFGGEYNYRDGV
                     SPWFRSIFEMRPDVDLMAAAPWGFKIHAVLAFLLFAMWPFTRLVHVFSAPVGYLNRPY
                     VVYRSRDARARGPQVRTPQGTWDKPGVKN"
     gene            18419..18880
                     /locus_tag="PKU75_02215"
     CDS             18419..18880
                     /locus_tag="PKU75_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPF79719.1"
                     /translation="MCSYCGCENIALIGRFMAEHVEIVNALTALRAACHDADAAAVGA
                     AVDAMEALLHPHTGAEEVGLFAQLRLQDEFADTIDSLCAEHVTLDAQLMAIRGGKHTA
                     YEQFEHDLRRHIDHEDNGLFPAAAIAMSGPDWEEALARTPGRGLDGRWDAG"
     gene            19108..20142
                     /locus_tag="PKU75_02220"
     CDS             19108..20142
                     /locus_tag="PKU75_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HPF79720.1"
                     /translation="MTDHTFRPTRRMAALLACAAIGLSACGGGEETASAGASGGGDAS
                     GVKIAAIFSGPVNDADYNALGLKALEAAKAKGAETSYSEKVAVPDVEARLTEAVGDGA
                     TIVWTHGSQFYDNTAKVAADNPDVTFIAEYDGTPKDQPANVWTIDRQFHLGFYGMGVL
                     ASKLSKTGTVGYVGGLSLPFSFAEVHAMEQAISDTGASTTIKPVWTGDFNDAAKAQQF
                     TQQLLGQSADVIVGSLNQGATGTFQAFEGKAAGDGWVTAKYTDKSAAAGDHYAGSLEY
                     DFVAPLAAILEKIAAGTKTGRYPLGFDTGVTIAMSDKVPAEAKTAVEDAISKVKSGAT
                     TVKLDVTKIG"
     gene            20150..21715
                     /locus_tag="PKU75_02225"
     CDS             20150..21715
                     /locus_tag="PKU75_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79721.1"
                     /translation="MSAPDAAPGGAAPPLLRMRGIRKTFGPVVALKDVDFTVGPREVH
                     GLLGGNGAGKTTLMNVLYGLYAPDAGTIEIDGAPVVIASPKDAIAAGVGMVHQTFLQV
                     DVFTVAENVVLGTDTSGVDVNGRIRELSERFGLAVDPAARIGDLPVGVRQRVEILKAL
                     YRGAKILVLDEPTTNLTPQEVDALFGSMRAIVDEGMSVVLITHKIRETMAACDRMTIM
                     RDGAWIATVDAADTSPEDLAARMVGAAPASDETADVALGGTEIGDATLDVPEVTGPVR
                     VVGSRLTVPGTGGKAVVEDVSIELREGEILGIAGVAGNGQVELAETLTGCRPAHGSLT
                     LDGTELVGRPTEAWLRAGVVYVPEDRQRDGILPGADITENLVLGAHRSRAKAGLIDWG
                     RARREAVEAISEFAVKTPSAATACGQLSGGNIQRVILARAFAHNPKMLLLHNPTRGLD
                     IASTQFVYDRIRAAAAGGCSVIVISEDLDEVIALADRIRVAYSGRLVGDFARGHADPY
                     ELGRLMTGVSGAA"
     gene            21712..22797
                     /locus_tag="PKU75_02230"
     CDS             21712..22797
                     /locus_tag="PKU75_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF79722.1"
                     /translation="MSTVTVAARPSVGERAKAAGVAAIPYILAVLVAFVVVAIIIALL
                     GHDPAKAFRAVLTNSFRNRTGMVQTLHKWVPLTLLALAFAIPLGAGRFNIGGEGQMIL
                     GATGAVAMGILLSDLPTVVLLPLLIVAGVAAGALWAGISAWLMQQFKVNEILSTVLLN
                     FVSFEILDYLASVVWSDPGAGGAVTTAIGAGGELPRIGKPPMHIGVILVALLAIAAAF
                     YARRSIAGFELRAVGLNERAAKLHGIRTGKVAVMSMIVAGALAGLAGAIEVAGVHHRM
                     LEGIQSNFLILGIIIGLIARGSLTAVPFVAFGIAVLEVGAGSMQRTSGAPVEMVLILE
                     GLILLFLLMSDWVTDRLRLRASRKAAA"
     gene            22794..23672
                     /locus_tag="PKU75_02235"
     CDS             22794..23672
                     /locus_tag="PKU75_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF79723.1"
                     /translation="MSDFITLTITAMVPFLFVAQGTMLSGRAGIFNVSQEGLMLLGAA
                     IGYLVSLKWGGNLVGLLVAALAGAAFGAILGWATTYLRMDQFVVGLALFFLSGGTASL
                     LYRVVIGADEPPRIRPLPDIVFGQDVLVFVAIGLSALLWWWLYRTDSGMRLRSVGENP
                     KAADSLGINVTRTRMLAASLGGGLMAVGGAYLPMAFSGVYADGIVAGRGWLAIALAFF
                     GGWRPQLILAGSLFFAAMDVLAQRAQVSGIGIPHQFVSMLPYVATLLVMIFAFRWALV
                     PQFLGKNYDRESRITG"
     gene            complement(23804..24781)
                     /locus_tag="PKU75_02240"
     CDS             complement(23804..24781)
                     /locus_tag="PKU75_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPF79724.1"
                     /translation="MPAITAADFSVLPRVAPVAPEAVSRPVLSVTSAPTGYEGEGFPV
                     KRAFAGIDLAHLDPFIMMDEMGEVNYAPGEPKGTPWHPHRGFETVTYIMDGTFVHQDS
                     HGGGGTITNGDTQWMTAGSGLLHIETPPESLVMSGGLFHGLQLWVNLPRADKLVTPRY
                     QDLRAESTALLSSEDGASIIRLIAGDVAGHQGPGSTHTPMTMIHATVAPGAELALPWR
                     RDFNALVYVMSGVGVVGPHSTPIRAGQAAVLGRGDALRVAAGAAADGQGQRGLEVILL
                     GGKPIREPIAWAGPFVMNTKSEVMQAFEDYQRGAFGQELPHTAASALAG"
     gene            complement(24969..25187)
                     /locus_tag="PKU75_02245"
     CDS             complement(24969..25187)
                     /locus_tag="PKU75_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79725.1"
                     /translation="MARYDINTTTMGALLDDPEVAAIMEKHAPGVTSNPMIGMVRGMS
                     AAQAIGMAGAMIGADKIEAIKADIDALA"
     gene            complement(25306..27072)
                     /locus_tag="PKU75_02250"
     CDS             complement(25306..27072)
                     /locus_tag="PKU75_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79726.1"
                     /translation="MPEGRNLILLLIFLLLLGVILWLLLTSRRRPETIVTREESRHDL
                     GGVDGLGRESAADAAVAAGAAGAVGAGTMGAAAWSAARGEDANEGAEALVGDFEAAEA
                     EADVAAGRTPHVWEETHPDRGSEATLTSAQADVEASDADADPEPGSAPELVADPDLET
                     DPDFVVGEDGVIPGSRLAGDAEVDGLAAAEAEARAATADTEMPSLDLPDLSADADDFD
                     LPEDGTLPGMTPDGAAADAAVAAGAAAPFDAPDVDASESAAGHVDAAGMDSESTAAAH
                     AEIDAAGLDEAGSLPEVDATGFDVAGDGADGPYVDAAGLDDEAAEPQNAAPVPAQDTT
                     DAEDIADAWAADDAGTPSSDIGMQAESAEAPMPVIPAPEEAEAEEIAAAWSGDDADAA
                     ADAPGDASVSEPSASHRFGLGESAGFDAQGGDLQADLSLEGERASAVDAANAAAEEHL
                     ATGAEVYGEEPEADRPDWAGPAAAGVAGARGAAAVSPARSYVSQPDPFHSQGAPEDSG
                     DAGSHEVIVHDHPVVDGGWSVGSAAPLEDGCMPLGHPIKGVYSLGIYQVPGSDWYDMT
                     VPDVWFMDEESAQRAGFRRGDG"
     gene            complement(27201..28829)
                     /locus_tag="PKU75_02255"
     CDS             complement(27201..28829)
                     /locus_tag="PKU75_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPF79727.1"
                     /translation="MDAPTLSHAKGEETPPLLRTTIDANLAETIARFPDRDAVVDVAA
                     GIRMTYAEFGAEVDRVARALRAAGYAVGERVGIWAPNSAEWVVVQYATARVGLILVNI
                     NPAYRTHELEYVLRQAGISLLVATPAFKTSDYQAMVEQVRGKCPDLRDVVFFGTESWD
                     RLLSRADEVSTEEVQAISDSLTCDEPINIQYTSGTTGFPKGATLSHFNILNNGYFVGE
                     ICRYTEEDRICIPVPFYHCFGMVMGNLAATTHGAAMIIPAPAFDPAATLAAVQQEKCT
                     SLYGVPTMFIAEWALPDFESYDVSSVRTGIMAGSPCPAEMMKKVIAAGIEEMTICYGM
                     TETSPVSTQTRTDDSFDHKVGTVGRVGPHLEIRVADALTGETLPRGVTGEFCTKGYSV
                     MLGYWQEEAKTAEAVVDGWMKTGDLAVMDEDGYVRITGRIKDMVIRGGENIYPREIEE
                     FLYTHPDVVDAQVVGVPDAKYGEELCAWIQMRAGAEQLTAETVRAFCTGNLAHYKIPR
                     YVMCVEEFPMTVTGKIRKVEMRARSTEELGLGTV"
     gene            complement(28931..30343)
                     /locus_tag="PKU75_02260"
     CDS             complement(28931..30343)
                     /locus_tag="PKU75_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPF79728.1"
                     /translation="MTSERPLSPDGLPEPDGADVLADLTQLLAPQFVIADPDRMAAYR
                     HDWSKGAGAGTPLAVVRPGTVEEVQAVVRWAAQRRIGIVPRGAGSGLSGGSLAVDGAI
                     TLSLDRMTRVEIDPAMRVAVVEPGALNITVKQAARELGLHYPPDPSSYEICSIGGNLA
                     TNAGGLCCVKYGVTTDYVLGLDVVLPDGTLVKLGGSTIKDVAGLSLLKLFVGSEGTLG
                     VIVGGTLRLIPAPSPAATLVATFPSVAAAANAVVALGNRLRPSLVEFMDNYSINAVED
                     LREMGLDRDAAALLLVQCDSPGAARAAEVLVVEEICQVAGASEVFSTTDPDEGEMFLA
                     ARRSSLPALERKGSMLLEDVGVPVPRLPALLARIEEISLRHGLPIPVVAHAGDGNTHP
                     NVIYDPAEPGALERAEAAFADVMAAAIELGGTITGEHGVGRLKKAALPAQLGPDVMDL
                     SRRIKAAIDPLGIMNPGAVL"
     gene            30505..30777
                     /locus_tag="PKU75_02265"
     CDS             30505..30777
                     /locus_tag="PKU75_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79729.1"
                     /translation="MSMLAEMQAAGGPHWNFERELNRAVGAALPGEPYATITRQAISS
                     YPSKDGLRVAVDQCAAYACAAGVVNATIPWDRLIAPDADMSNQWGH"
     gene            complement(30844..32271)
                     /locus_tag="PKU75_02270"
     CDS             complement(30844..32271)
                     /locus_tag="PKU75_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPF79730.1"
                     /translation="MTRADDILTALEALRANDVATHGGRTLSYVYDSGLADADEIGRR
                     ALASFAGTNGLDPTAFPSLLAMENDLVRLAGHLLRAPDGFAGTVTSGGTESILLAVLA
                     ARDDPKNSAAQRDSPSMVLPTTVHAAFHKAAHYFGVRPVFVNVDPVTFRADPAAMAAA
                     VDGTTVLLVGSAPSYAHGVIDPITALADVAGEHGIRLHVDACIGGWVLPFLDGAPPWS
                     FEIEGVSSISVDLHKYGYTPKGVSILLHRNASLRKSHFFAAADWPGYTMLNTTMQSTK
                     SGGPLAAAWAVVRYLGLDGYRELAQVAREATVGLAEQIAERGLGLRPLVAPDSTLLAV
                     TTTQDAGYDVFTLADEASSRGWLLSPQLPFRGGPPSLHLSLCAATAPRVEELLEVLAE
                     SAAAARAAGPVSVDPAIAGVLAHLDPAALDEATFDGILALAGLGGGCALPDRMAEINA
                     MLAQAPPRLREALLLAFIDRLTRPT"
     gene            complement(32268..33623)
                     /locus_tag="PKU75_02275"
     CDS             complement(32268..33623)
                     /locus_tag="PKU75_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79731.1"
                     /translation="MTQSRSVAPDALPRRVRIGYGLGGVATGSFGTVPGLLLLPYLTD
                     HIGVGAAIAGLIVLLPKAWDVVLNPIAGRLSDRSAHPDGRRRPFLLRAGLVLAGCFVL
                     LFAGPSAPTWLGGGWVALAFLACATAYAFFQVPYVAMPAELTSDYAERTRLMTWRVAI
                     LALAILLSGGLSPAIRDAVGGAWGYRAVGIFVALLIAAGAYAAWRGTAGVRLHDVAAP
                     VAGLGEQLRLVAATPDFRRLLTAYVLQALATGCMLAGVDYVARWVVGGGGAATVIFVC
                     FVAPALVVMPLWQAVGDRLGKKGGFLLASLVLLTGALAIFVVREGSLALLAAAVAVVG
                     IGYAGTQLFPLAMLPDVAAVDAARTGENRVGVFTGVWTGAETLGLALGPGLYAAVLAL
                     GGYVSSSGDSMRQPAGAIDAIQLGFSLVPAVVIALSLLALARYRLDESAVRAASEEGI
                     V"
     gene            33746..35404
                     /locus_tag="PKU75_02280"
     CDS             33746..35404
                     /locus_tag="PKU75_02280"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HPF79732.1"
                     /translation="MTLPSVAADPDATAGAAAVAAAARAGVRTMFTLSGAHVFPLYDA
                     AHRGAAPLRMIDVRHEASAVFAAEAVGKLTRTPGFAVVTAGPGVTNAVSPVTQAHFSG
                     APVVVFGGRAPELTWGRGALQEMDQAPLFTTITKSSATAHTPDEVGALTAAAFTAAST
                     AHRGPAFVEAGIDVLFAPAGDVGTAVPGPGGRPAPDEESVAEIARLLREARRPIVILG
                     SDVWADGAEQAALDFVTALDLPAIGNGMGRGILPPTHPGCVTRARRAALTQCDVAIVV
                     GAPLDFRIGYGTFGPAGTDTPVIHIADSTDQIATHRTLAASAAGDLGGILRTLCDLVG
                     GPGGWREWRSGLREQADAAGAADAPLLTAGGDLIHPARIYGELLPRLGDDAIVIGDGG
                     DFVSWAGRFVQPARPGRWLDPGPFGCLGAGLGAAIGARAADPDAPIVLLLGDGAAGMS
                     LMDLDTLVRHDLPVVIVVGNNSAWGLEKGPMQLLYGYDILADLAPGTRYDKVASALGG
                     ASELVDRPGDIGPALDRALASGTPYLLNVLTDPAAAYPRSTTGL"
     gene            35485..36705
                     /locus_tag="PKU75_02285"
     CDS             35485..36705
                     /locus_tag="PKU75_02285"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPF79733.1"
                     /translation="MKPIVQSRKLQGVRYDVRGPILVEAQRLEAEGHKILKLNIGNTA
                     PFGFEAPEAILADMIHNLPEAQGYSDSRGIYSARTAVAQYYQSKGFKDTLVDDVFIGN
                     GVSELISMVLTAFIDDGNEILVPAPDYPLWTGAVTLAGGIPVHYRCDEDNEWNPDLAD
                     IESKITENTHAIVIINPNNPTGAVYSEGIVRGLVDIARRHDLVVMADEIYEKIIFDDA
                     VHHHAATFCGDDVLCLTFSGLSKAYRVCGYRAGWVMVSGPKHMATDFLEGLTLLANMR
                     MCANVPAQHAIQTALGGYQSITELVVPGGRFYEQIKLASKLLNDIPGVSCVEPKGALY
                     CFPRLDPKVYPIEDDQQFVIDLLRAKKILVTHGTGFNWFEPDHFRLVTLPDTEVLTEA
                     IHRIAAFLETQRAG"
     gene            36766..37290
                     /locus_tag="PKU75_02290"
     CDS             36766..37290
                     /locus_tag="PKU75_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79734.1"
                     /translation="MSTRPGPESRAVNTSGKWILTAFCLAALAVFALLVPSVREWLAR
                     DDDSQLSAIELTISPATSKEVDACAPVSQDALDRATTAVTGTYEGISDGAATFVADHW
                     FRGGPAERVVISSSSSANLDRALEQAQLNTGRVLIASRSGEVLLCGASGPYTSELASF
                     YVQTYEQRTASPTS"
BASE COUNT         5938 a        13087 c        12660 g         5674 t
ORIGIN      
        1 cctgcgccac ggtcataccg cgcaccacgg tgtcgtccat gtcgaggacg gcctgaccgt
       61 ccttctcggg ggtgaccagc ttcatcaggt tgacgaggtt ctgcccgaag agctgggagg
      121 cctgggtcgg gaggcggccg ggcaggtcgg tgtagccgag gatcttcacg ccattggccg
      181 ttgttacgac ctgatcggct tgtgccccag cgacattgcc gccattcgac gctgccatgt
      241 cgacgatgac cgagccgggc ttcatcgagg cgaccatggc ctcgtcgatg atccgcggcg
      301 cgggacgccc ggggatgagc gcggtggtga cgatgatgtc ggccttcggc acctcctcgc
      361 gatacatctc ggtggtccgc ttgttgaact cctcgcccat ctccttggcg tagccgtcgg
      421 cggacgggcc ggtgtcgagg ccctcgaggt tgggccgcaa gaactgcccg cccatcgact
      481 cgacctggtc ggcgacctcc tggcgggcat cgaacgcctt gacgacagca ccgaggctgt
      541 tggccgtgcc gatcgccgcg agcccggcca cgccggcgcc gacgacgagc acggtcgccg
      601 gcggcatctt gccggccgcc gtgacctgcc cggtgaacat gccgccatac tcgttggccg
      661 cctcgatgat ggcgcggtag ccgccgaggt tggccatcga cgacagcacg tcgagggcct
      721 gcgcgcgcga gatacgcggc accgcgtcca tggccatcgc cgtgacgccc ttggcgcgca
      781 acgcttccac gagttggggg ttgagcgccg gcgccatcag cgacatcagg atctgaccgg
      841 agcgcaaccg ctcgatctcc tccgcgtccg gcgcgttgat cttgaagacg atgtcgccgg
      901 catacgcctg ctcgggcggc acgatcgccg ctccggcagc ggtataggcg ctgtcctcgt
      961 aggccgaggc gcgcccggct ccggtctcga cggccacgtc gtaaccgagc ttgatcagct
     1021 gctcgaccgt cctgggcgtc gcggcgaccc gggactcgcc gggtcgggtt tccttgggca
     1081 caacgatgcg cacgagcgtc ccctcatctg tgggtttcct gctgcatcga acctactcgc
     1141 gtgccctccc cgggtgccaa ttctggacgc ccgccggggg tatgccgttc gccacctttt
     1201 cgggtgccgt gccgggtggt atgccgctca gccggtttcg gcggcgctcc cgccggggcg
     1261 gctgacgtcg acgccgtatg ccgcggcgag ccgctcggcg gcgagcgcct cgtccgtgcg
     1321 cccggcgcgt tgcaacgagc ggacgaggat gagcaacgca taggaattgc ccgggtcgcc
     1381 ctgcaggatc tcgtgtgccg tgtccaccgc gcgcatcagc tgcgcagagt ggaagtagct
     1441 gcgcgccaac aactctcgcg cctcgccgag cccgggctcg gagccatggt gttcgagcag
     1501 gtactcgagg acctgccccg cctcgatgta ctcgcgaccc tcgaagagct cctgcgccca
     1561 ggtgtagatg tcatagggct cgcgcccttc cagcatgcgc acagccaagg tcatgttcgg
     1621 ctccctccga ttcccattgg gcatgggtat gccgcctgaa acaccagcgg ccgcgccggt
     1681 gttcccgagt caggcgagcg gctcggcgag cgcggcagtc accgacgcga acatccgctg
     1741 cttgatcgtc ccgagcgtgc cgcggtcctt gccgaccagc gagcgggcca gctcgatcgc
     1801 tgtgggcagc acctcctcct cgctcgccac cgcgtccacg atcccggccg acaaggcctc
     1861 cgcaccgcca taccgccggg cggtcgtcat cgcatcgatc gccgaccgcg gtgtcagctt
     1921 cgactgcacc aacgcgctca tgccgacact gaacgggatc cggatgtcgg cttcgggcag
     1981 gcagaagaag ccgcggtcgc cgcgcatgat gcggtagtcg tggctgaagg cgatcagggc
     2041 cccgccgccg aaggcgtggc cgttgatcgc gcacacggtc ggcaccggga gtgtcagcac
     2101 ccgtgacagg aactcctcca cccgcttgac ataggggaag aactgatcgg gatgcgcctg
     2161 cacccaatcc agatccaggc cgttggagaa gaacttgccg gttgccgtgg tgaccagcgc
     2221 gccgggctcc gtggcggcct cgagttcgtc gagatggccg ttgacggcat cgagccagtc
     2281 cggcgagaaa cggttctcgt cggaaccgaa gtcgaggatc cacagcgagt cgtcgcgacg
     2341 cagttcgggc atagcgcgat cctgccacgg catacgatcg gcgccatgac cggctcccac
     2401 cccctcgatg tgcttttcga cgcagcggcc gcggggagtg aggtggagat tcccgagggc
     2461 tgggggcagg gccgagccgt gtatggcggc ctggtcgccg gcacgctgca agcgcacgcc
     2521 ctcggggcct tcgacctgga gccggatgcc ttgcgggcga ccacgacatc cttcgtcgcg
     2581 ccgaccgagt gcggtccggc ccgcctggaa gcggtgctcc tgcgccgtgg ctcgtcggcc
     2641 acgcaaacgg aagtcaaact gtggcaacgc gattcgcgca gcggcgagga gacggtgcgc
     2701 tcggtgcagc tgtcggcgta tggcgctgac cgggagtcga gcgtcgacct ggctccggcc
     2761 ccgccgcggc atacgatccc cgaccccgac ccgctgatgg tggttccggt cgttcccggg
     2821 atcacgccgg agttcttcgc caaggtgcag ttgcgcccgg cgcacggccg attccccttt
     2881 tccggcagca cgactggcga catggccggc ttcatgcgcc tgaccgagac cccgcgcgcc
     2941 ctcgcgctac cgcacttcat cgccctcgtc gacgcctggc cacccgcccc cggacagctg
     3001 ctgcgctccc ccgcggcgat gagcacactc acgtggacgc tcgaaatgct cactctccca
     3061 accggtcccg cggaccaaca ctggtggtat gaagtggaca ccgacgccgc ccacgacggc
     3121 tacgcgcaca cgcacgcaca cgtcttcacc cgcgacggcg cgccggtcgc catctcccgg
     3181 cagacgatca cgatcttcgg ctgagcatcg ggcgtggcac cctcggcggc atgaacagcg
     3241 agcgccggcg gcataccgtc gccgcgctgc tcgccgccgg actgtggggc acgacgggga
     3301 cggtggcgca tcatgcgccg gccggcagca accaggctct ggtcgggctg gcgacgttcg
     3361 acatcaccgc cgccgaacgg cgggccacag cgcccacccc ttgattacga tcgtcgagtg
     3421 acaccggagg tcgtggccgc cgcgcgcgtg ccgtggacaa gccccgccat ccgctcctgg
     3481 tattccgcgc cgagcctcct cgcgcagctc gccgatggcg tgcgccagga ggtcgcgtgg
     3541 gtcggggacg aggagttcgg ggccgagttg cgaggccacg tcgactcggc ctcaccgatg
     3601 ccgccactgg cttgggcgaa ccgagtcctt gacctacccg acggcgggtg ggccttgtgc
     3661 ggcatacgtt ttcgccagcg cgacaacgcc cgccccttcg tggacgttgt cgccagcact
     3721 gctcccccga cccctgacgg actggcaacg atcgccgccg cggtggcgcc ggcatacgac
     3781 ccgtggcacc cgctcgccct gcgcgcgaac ctgcccgatc cggacgcgat cattgctgcg
     3841 gtgcggggag attcacgctt cgtggggatg tcggtcgata tgtatgtgat ggccggtctc
     3901 gtgcaccggc tccgcactcg gaagcgggac atcgacgacc ggctccggct ggtccccggc
     3961 agcccgggtg aactcgccgc gcgcaacgcc gggatctacg gcgagctcta tcggcgggac
     4021 ccggagcgcg cccgctgggc cacacccgag gacgcggact ccctggccga ctgcgccgac
     4081 gagggcttgc tcttcgaggt gcgggtgcac gacgagcccg ccggagtggt cgcggccatg
     4141 cgatgggacg cgcacggcat gtgcggcttc tctgtggagg aactcgctct ggatgccgag
     4201 catcgcgaaa agggactggg gcccgttgtg ctgcaacgac ttctgcgcaa actacccgcg
     4261 gacgacgggg atgcgctgtg gggcacgatc caccacaaca acatcccgtc cctgcgcaac
     4321 gccctgcgcg tcggccgcga gatcgtcggc gccaacatgt ggatcactcc cgccggctgg
     4381 tccggcatgc cttagcagca gtcgacggac agcagggcgg agagggcagc cagggcttcg
     4441 gggcggatcc agtagtagac gaaggtgccg cgacgttcgc tgtcgaccag cccggcatcg
     4501 cgcagcgact tcaagtgatg gctgatcgtg ccggacgaca gatcgaaggc cggggtgatc
     4561 tcacagacgc agacctcggg ctgcgtggcg atgagggacg ccatccgcag gcggaccggg
     4621 tcgccgagag ccttgaacat cggcgcgagg cgttcggcgt cggccgcgct caccgggatc
     4681 ttggtgagtg cgcagcaggt ctgggccggc tgcgcgccgg ccggggccga attcgtcacg
     4741 ccgtctatct tgacagatat cgaatcagag gtccacgatg gattttgatt cgataatcat
     4801 caagacaagg agcaatcgat gtccagcaat ctcaccgacg atcaggtccg cgacgccgtc
     4861 cgggagcggt atgccgcgtc ggctcacctg tccctgtcgg gcaaggtcga ctcctgttgc
     4921 ggcggaacgg ttcccgcgcc tggcgagtcc agttgctgcg gcgaagccgg caaggcgcgc
     4981 ggcctcgacc ccatcacggg cgggctgtat gacgagacgg atgtcccctt cgacgccgcg
     5041 ctcgcggcgt cgctcggctg cggcaacccg acggcgctcg ccgagctggc gcccggcgag
     5101 gacgtcctcg acctcggctc gggcggcggc ctcgacgtgc tgctctcggc acgccgggtc
     5161 gggccggagg gcacggcata cggcctggac atgacgcacg agatgctcgc gctcgcggag
     5221 agacacaaga cggaggccgg catcgagaat gctcagttcc tgctgggcac gatcgagcag
     5281 gtgcccctgc cggacgcgag cgtcgacgtc gtcatctcca actgcgtcat caatctctcc
     5341 cccgacaagg acgcggtgct gcgcgaggcc ttccgcgtgc tgcgccccgg cggccggttc
     5401 gccgtctccg acatcgtgct cctgcgcgag atcccgcagg agttgcgcgg ggtgctggcc
     5461 ctgtggaccg gctgcatctc cgggtccctg agggacgagg agtatgtcgc caagctcgcc
     5521 gccgcgggct tccacgacgc gggcgtcgag gtgacccggg cctattcacg ggccgacctc
     5581 gaagacctgg ccgatcagat ggacctgacc gcactcccgg agggaatgga cccggccggc
     5641 gcgatagacg ccctcgatgg cgcctttgcc agcgccttca tccgggccac caagccctga
     5701 cagcccgtca ggcggcgcgc cggtcctggg cggggtggcg gtcggcggcg ggggtgaccc
     5761 accgcgcttc catacgggtg cgtccatccc gtcccgtgac cggacgccat tgcatgacca
     5821 gtttctgact cttgtcgctc atagtgtgtt caccttccgt tcactttcag ttcacccacc
     5881 gtacgcggtc aaccccctgt tgaccaaatc gactcgcaac cccgcgtgtc cggaccgcgt
     5941 gggacgctgg cctggtgccg cctaccgccg cgacgactcg ccgaatcgtg cggtcaccgg
     6001 cccgcgaggc gtgctcgctg ctcccccacg agcccggtgt ctatcgcttt cgagacgaac
     6061 gcgaccgggt catgtatgtc ggtcgcgcca gcgatctgcg ctcacgcacc cgctcgtact
     6121 ggggcgacct gcacggccga cggcatctgc gccacatggt ggggcagatc gcgcgcgtcg
     6181 aggccttggt atgccagtcg gtgcatgagg ccgcgtggct ggagcgcaac ctgctgacac
     6241 gctctctggc tcgctggaat cggacccgcg gcgggcagga agtgccggtc tggttcgtgc
     6301 tcgatcccgg gccccgggca ccgtccctgt cgctcagcta cgggccgccg cccgacccgg
     6361 agctcggttt cgggccctac ctgggcagca cgcgagcgcg cctcgcccgc tccggcgcgc
     6421 tgcgggcatg gccggtgcac ctgacgggaa cgcacctgga cgccggcacc cgctcgatcg
     6481 gtgaggcccg cggcgtcgat ccgtcccagc gcgcagcggt gatcgccggg ctcgtcgggt
     6541 tgctgagggg cgtcgaggac gatgtcgcag ccacccgcgc ccgcctcgtc gagtcgcgct
     6601 cgcgcgccgc cgcccagctc ggttacgagg tggccgggca gatccaggaa gaactcgccg
     6661 cactcgagtg gagcgtggcg ccacagaccg tggcggggtg cacgcctcct gacctgcgtg
     6721 tcgcggcctg gcacgacggc ctgctgctca cgttgcgggc acgcgacggt ttcgtggacg
     6781 tgtggcgcac gcgcgactgc ccggcccgtc aaggtgccca actgatcgcc gccacacccc
     6841 ccggctggca gcagttcgcg gaccgcaacg tccgcctcct ggttgctcta cgcgatgccc
     6901 aggccggcgc aaaccgggcc tgatccctcg aatgcggttg cgggccaaga cgaggtcagt
     6961 cgaggtcctc gccggccagc gccttggctg ccagccgctc ctcctcgctg acctcccaga
     7021 cctcatgagc cgcgcgcagg gccagcgcga tgatgaagac gccgaggatc aggtcgggcc
     7081 agccggtggc gttccacacg gtgacgaccg ccatggctat gatcgccaga ttcaccagca
     7141 cgtcgttgcg ggcggacagg aacgcaccct tgctgagcga accaccgtga tgccgcacgc
     7201 gagccagcag ccaggcgctg gtgccgttga ccacgatggc tccgagggag gccagcacga
     7261 tcggtggtgg gtccggcgcg gccgggtcgg tgaagcgttg gatcgcctcc cagcaggcga
     7321 cgactgccgg gacgaggacg acgaccgcca tcgccttgcc ggccaccgcg cgcctggcca
     7381 gcggccagcc cagggcgatg aggatcagca ggttgacggc ggtgtcctca aggaagtcga
     7441 cgctgtcggc gagcagcgag accgagccgg cgacctgggc gaccgcgaac tcgacgaaga
     7501 agtaggcgaa gttgagcgcc gccaccagcg tcaccgcccg gcgcaactca cccgactcga
     7561 tcgtgggtgc gggctgactg tcagcgctca tcggtggttc cctccgcgga cgtgtgcgac
     7621 ccgggacagt ccggcgccgg ccgtgactcg aggtcacagc cggcgcggga cgtcgccggg
     7681 atgaccccta cgacggcttc ggagcacccg gacgggcgta gcgcatccac acgatgatcg
     7741 tgcacatgat cgcccaggcg acaccgatcc agtagaacgc cgtcgggccg agcgagctga
     7801 cgcccacacc gaagaagaag ggcccgaagg cggcgatggc ggccgtccag ccgatcacgc
     7861 cgccggcctg gcggcgctcg aagatcatcg gcatctgctt gaaggtcgag gcgttgccga
     7921 tccccgagaa gaggaagatc gcgagcatcc cccacaggaa gtggttgaag tcgcccttga
     7981 gcgccgcggc cgaggagaag tcgggcgtga gggccgggat cgtgtagatg atcgaggcga
     8041 tgaggccgat gccggagatg agcgaccaga tcgcaccgcc catcttgtcg gtcagcggcg
     8101 agaagagcac gcgggcgccg gcgccgatca acgggccgag gaagacgtac ttgaccgggt
     8161 cgggcacggc atacccctcg atcaaaacct tggcggcagc gccggttccg gacacgacct
     8221 tgtcattgcc cgagccgtag aggttggcca tgagcaggcc gaactgcgca gccaggccgg
     8281 agaaggtgcc gaaggtcatg atgtagatca gcgtcatcca ccaggtgtcc tggttggaga
     8341 agatgtcgaa ctgctgcctg atattcgcct tgaccggcac cgacttcagc atcgtccacg
     8401 cgagcacggc gccgatgatc atcagcggaa tccacacgaa ggcggcgttc tggaagtaga
     8461 cgttcttgtc gggcttgccc gcagtcttga gcacctggga gccgccgaag atggccagcc
     8521 cggacatggc gatcagatag ggcgtgagga gttggacgac cgagacaccg aaattgccca
     8581 ggccggcctg cagaccgagc gcggtgccct gcatccggcg cgggaagaag aaggacgtcg
     8641 agggcatgaa gccggagaag aggccgccgc cgatgccggc gaggaaggcc agcaccatca
     8701 gttcccaata gggcgcggtc gggtgctgca cccgcacgcc ccacccgagg gtggaggcga
     8761 tcagcaacaa cgtgctgagc gagaccatct ttcgcgtgcc catgatgggc ggcagcacca
     8821 tccagacgag ccggaacagg ccggcggcga ggccgggcat ggcggccatc cagtagagct
     8881 ggttcttcga ccatgagtag cccagggagt tcagcttggg gatgatcgcg ctgggcagga
     8941 accaggcgat gaacgcgagg gtgaggcaga aggtggtgat ccagagggtc cgccacgcca
     9001 ggcctttgtc ccagcgtgcc tcgtcttccg gatcccaggc ctgcagccac tccccgccgg
     9061 tgcgggtcgg tgcggtgctc atggtgggga ttctcctcgt gttgcagggt ttttgggggt
     9121 ggtatgccgc gcggccgcct tcgcgtgtcg cgcgagcctg tcaccgggtc gcggctggcg
     9181 cttccaggtg gcgggccagc tcggggttct ccttgtgcag catgtgcacg acggtccagt
     9241 gcatccagac ggcgcagacc gcggtcagca ggaagaggac gaagaaggtg ctggtcggga
     9301 aaccgctcca ctgcttggtg taggcgaaca gcggcggcag gacgaagccg ccgaggccgc
     9361 cgaacatgcc gacgagcccg ccgactgagc cgacgttgtc ggggaagtat tccggaatgt
     9421 gtttgtagac agcggctttg ccgatgccca tggcgcaccc gagcaggaag acgagggcga
     9481 cgaagaacgg caggctgatg tggtagccga ggtggtcgct ctggctgccg tcggcgtggg
     9541 cgatgacgat gtggccgttg ggcatcatca ggatgccggt gacgagcagc atgacgccga
     9601 aggtccagta catgacgcgc cgggcgccga agcggtcgga gaaccagccg ccgacgggcc
     9661 gcagcaggga ggccgggaag atgaagatcg ccgttagcag ggcagcggtc ttcagttcga
     9721 tgtcgaagtt atcgacgtag tacttcggca gccaggcgga gagggcgaca taggcgccga
     9781 agacagcggc gtagtagagg ctgaaccgcc agacgcgaat gtgcccgagg ggagccaaca
     9841 tttccccgat cggcttgccg gcgccggcga cccggtcctg gcgcggcacg acgaggtaga
     9901 cgagcaacgc catcgtcacc agggccacgc aatagatcgc ggggaagatg cgccagccgc
     9961 cctcgaagat gccgaagtag gtgtggccga ccgtgccggc gatgatggcg gggaagagga
    10021 cgagcaacag cttggtcacc gacgccccga cattgcctgc gccgaagacc ccgagcgcga
    10081 agccctggtg ctgcttcggt gtccacgccg agttccaggc gatgccgacg ctgaagaggt
    10141 tgccggcgaa gccgacgagg aaggcgagga tcagcagctg ggtatagccg tgggcccggg
    10201 tgagcaggaa cgccgggatc gccgtcagca gcagcatggc gatcgtgacc ttgcggccgc
    10261 cgatccggtc ggcgagcatg cccgccggca gccgccagat cgacccgttc agcacggcga
    10321 cggccgagag ccacgccagc tgcgggtcgg tgagcttcag ctccttctgg atcggcacac
    10381 cgaggatgcc gaacatcagc cagacggcga acatcaatgt gaacgcggcc gtcgacatcc
    10441 acagcacgcg ggaggcgccc gcgtgccgct cgggggtgtc ggcgtcgacg ggggtggcac
    10501 cggtgggggc gtccgggcga gccatgaggc ggtctcctcg agagtctctt ttataggagt
    10561 taagtccgtt aaatcggacc cgaagagacg ctaccccgca caacgaattt cgcctacgaa
    10621 ggcgcaccta accgcgtgtc ggggggccga cgtgcttgaa taaacaggaa gtcgatccgg
    10681 ctatctgtca ggaggcgagg atgagcgacc cggcactggc cgctgcactg ctgtcttcgc
    10741 ccgtgcgccg gcagatcgtc gacgcgatcg ccggcgccca agccgcgcgg gtgcccggca
    10801 ccggtgcccc cgctctcggc gccgagggcc tgagcgctgc ccaggtcgcc aaggccgtcg
    10861 acctgcacgt gacgaccgcg cgcttccacc ttgaccagct cgtcgccgcc ggcgtcctgg
    10921 tcgcgtcctt ccacaaacat cccggagccg ggcgcccccg caaggtgtat gccgtgacac
    10981 gacgcccggt gagcgccgaa aaccccgagg ttccgaccgg cttgctcatg gaggtcctcg
    11041 ccggcacgct cagcgccggc cgcgatgagg gccgggtcgt cgagcccgac gaagccgggc
    11101 gccggtgggc gctggagaac gtgccggccc acgacgcgtc gccggcgcac tcggccggcg
    11161 agttcgtcgg caaggtcggc ggcctgatcg acgtcctgca gcactggggc tacgaaccag
    11221 agttgtccac gcgtgaccaa ggccgatccg tgcagatcga tctgccccac tgcccgttcc
    11281 gggggctggc ccgacggcat actgacgtgg tgtgcggcat ccatcgtggg ttgatcgcgg
    11341 gcaccatgga ccggctcggc gagccgaata cgacggtgac cctgctcccc ttcgccgagg
    11401 gcggcacgtg catcgcgcac atttcgcgca ccaccccttt cgacccggct gagccccagc
    11461 cgcgcccgac cgaccccgac cggaagcaca ccaggaagca cgccgatgac tgacactggt
    11521 ttcgactccc cgctcgccga cgccctcgtg ggcacccggc ggttcttcac caaggcgcag
    11581 gtctccgacg acaaacgcag catgtacgtc gcggggggcc gcagcggcga cagcttctat
    11641 cgcgaccgct ggagccacga caaggtggtg cgctccacgc acggggtcaa ctgcaccggc
    11701 tcctgctcgt ggaaggtcta cgtcaaggac gggatcatca cctgggaggc gcagcagacg
    11761 gactatccga gtgcgggcgc ggacaagccc gagtatgagc cgcgcggctg cccccgcggg
    11821 gcctccttct cctggtacac ctactccccc accaggattc gctacccgta tgtccgtggg
    11881 gtcctgctgg agatgtaccg cgcggccaag caggcggtcg gcggcgaccc ggtggtcgcc
    11941 tgggcctcga tcgtccagga ccccgataag gccgcgcgct acaagagcgc ccgcggcaag
    12001 ggcggcctgg tgcgctcctc ctgggaggag gtcgtcgaac tcgtcgccgc cgcgcacgtc
    12061 tatacggtca agcgctgggg cccggaccgg atcgccggct tctccccgat ccccgcgatg
    12121 tcgccggtgt cgtatgccag tggcgcccgc ttcctcgaac ttgtcggcgc gccgatgctg
    12181 tcgttctacg actggtatgc cgacctgccc aacgcctcgc cgcagatgtt cggcgaccag
    12241 accgatgtgc ccgagtcggg cgactggtgg gacgccggct acctgatgat gtggggctcc
    12301 aatgtcccgc tgacgcggac cccggacgcg cactggatga ccgaggcgcg ttatcgcggc
    12361 cagaaggtca tcgcggtctc ccccgactac gccgagaacg tcaaattcgc cgacgagtgg
    12421 gtggcctgca accccggcgc cgacggcgcg ctcgccatgg cgatggggca tgtcctgctc
    12481 aaggagttct tcgccgattc ccaggtcgac ttcttcgtcg actacaaccg caagttcacc
    12541 gacctgccga tgctcgtgac gctcgaggag ggcgaaaacg gctgggagca agggaaatac
    12601 gtcgtcgccg gtgatctcga tgtcccggag aaggactccg agaacgcgat gtggaagacc
    12661 gccgtgctcg accagcgcac cggccaggtc gcgatcccga acggctcggt cggctaccgc
    12721 tatggcgagg agggctgggg caagtggaac ctcgacctcg gtgacatcga gccggccctg
    12781 tccgtctatg acacccgcga cgacgccgtc ctcgtgcgct tcccgcgctt cgacgccgac
    12841 ggcggcagcg tcgtcttcga gacccgcggc gtgccggtga agcgcatcgg ggaccgggtc
    12901 gtcaccacgg tgctggacct catgctcgcg cactacaaca ttgcgcgcga agggcttccg
    12961 ggcgactggg ccacctcata cgatgacgcg gatgccgtca acacgccggc ctgggccgag
    13021 cggatcacgg ccgtcccggc gagccagatc atccggctcg cgcgcgaatg gacgcacaac
    13081 gcgatcgaca ccggcggccg cggcatgatc ctcttcggcg ccggcatcaa ccactggttc
    13141 cacgccgacc agatctatcg cgccatgctc gtgctgacga cgatcaccgg ctgccagggc
    13201 cgcaatggcg gcggctgggc ccactatgtc gggcaggaga agatccgccc gatcatgggc
    13261 ttccagcaca tggccttcgc cctcgactgg caccgcccgc cgcggcatat gaaccagacg
    13321 gcctattggt atgtgaacac cagccagtac cgctacgaca ccttcaccgc cgacgacctc
    13381 gacatgggca ctggcgtctt cgctggcaag ggggtcatgg acctgctcgc gctgtcggtg
    13441 cgcctgggct ggacgcccag ctatccgacc ttcaaccgca gcagcctggc cctcgccgac
    13501 gaggcgaaag ccgctgggat ggaaccggtt ccgtatgtcg tggagcagct gaagtccggt
    13561 gagctgaagt tcgcggtcga ggacccggag gccgaggaga acttcccgcg cattctgagc
    13621 ctgtggcgct ccaacctctt cggttcgtcc gccaagggca acgagtactt cctcaagcac
    13681 ctgctcggca ccgacaacgc cgtcaccgcc tcgcaggcgc cggccgacaa gcggcccgac
    13741 ggcatcgtct ggcccgagca ggtcccggag ggcaagctcg acctgctgac cacgctcgac
    13801 ttccggatga cgagttcgac gctcttctcc gacgtcgtgc tgccggcggc cacctggtac
    13861 gagaagcatg acctgtcgac gaccgatatg caccccttcg tccactcctt caacccggcc
    13921 atcgcgccgc cgtggcagtc caagtcggac tgggacagct ggaaggagat cgcgaagaag
    13981 ttctccgagc tggccgtcga tcatctcggc acgcgcaccg atgtcgtggc gaagccgctg
    14041 tggcacgaca cgccggaagc ccttgccaca gtgcacggtt cggtgcgaga ctggcgcacc
    14101 ggcgaggtgg agccggtgcc cggcaaaacc ctcccggtgc tcgcggtcgc cgagcgcgat
    14161 tacaccgccg tctacgacaa gatgacctcg gtgggcccgc tcatggagaa ggtcggcatg
    14221 ctgaccaagg gcgtgcccta tgacgtgcag cgagagaccg agatcctgcg ccagcgcaac
    14281 ggaactcagc gcggcggagc cggtgacggg cagcccaaac tcgacaccgc gatccacgtc
    14341 gccgacgcga tcctgcactt ctccggcacg gccaacggcc atctcgcgac cctggggttc
    14401 aagaacgtcg agaagcgcac cgggacccaa ctgcacgacc tctcggccga gcacgagggc
    14461 aagcagatca ccttcgccga cacccagatc cagccggtgc ccgtcatcac ctccccggag
    14521 tggtcgggct cggagtccgg tggccgccgc tatgccccct tcaccatcaa tatcgagcgg
    14581 ctcaagccct tccacaccgt ctccggccgg cagcagttct atctcgacca cgactggatg
    14641 cagggcatgg gcgagacgct gccggtctat cgaccgccgc tgaacatgac catgctcttc
    14701 ggcgaaacgc cgctcggcga gaagacggaa ctcggtgtct ccgtgcgtta tctgaccccg
    14761 cacaacaagt ggtcgatcca ctccgaatac caggacaacc tcttcatgct ctccctctcc
    14821 cggggcgggc agacgatctg gatgtcggac cgggacgccg acaaggtcgg gatcaaggac
    14881 aacgactggg tcgaagcggt caaccgcaat ggcgtcgtcg ccgcgcgggc gatcgtcagt
    14941 caccggatgc ccgagggcac ggtctatatg catcacgccc aggaccggct catcgacgtg
    15001 ccgctcacgg agaccaacgg ccggcgcggc ggcatccaca actcgctgac ccgcatcatg
    15061 atcaagccga gtcacatcat cggcgggtat gggcagttcg cctacttctt caactacatc
    15121 gggccgacgg gcaacaaccg cgacgaggtc acgatgatcc gcaaacgcac cgcgccggtt
    15181 cggtacaact gatcgcggcc acacggcata cgagaaccta agggagagaa cgacaatgcg
    15241 cgtgatggca cagctcgcga tggtgatgaa cctcgacaag tgcatcggct gtcatacctg
    15301 ctcggtgacc tgcaagcagg cctggaccaa ccgcaccggc atggaatacg tgtggttcaa
    15361 caatgtcgag acccggcccg gtctgggcta tccccgacgc tggcaggacc aggccgagtg
    15421 ggaaggcggc tgggtccgca aggccaacgg ccggctcacg ctcgccgccg gcggccggat
    15481 gaagaagctg ctgaacatct tctccaatcc caagctgccg agcatcgacg actactacga
    15541 gccgtggacc tatgactacg agacgctgct gaattcgcct cagcagcaac agttcccggt
    15601 tgcccggccg ctgtcgctga tcagcggcaa gccgatgaat gtcacgtggt cggccaactg
    15661 ggacgacgac ctcggtggcg cccgggagta cggtccgaag gacccgatga tcaagggcat
    15721 cgaggacaag gtcaagttcg aattcgagca gaccttcatg ttctatctgc cgcgcatctg
    15781 cgagcactgc ctcaacccgg cgtgcgcagc atcctgcccg agcggcgcga tctacaagcg
    15841 ctccgaggac ggcatcgtgc tcgtcgatca ggagcgctgc cgcggctggc ggatgtgcat
    15901 ctccggctgc ccctacaaga aggtctactt caaccacaag accggcaagg ccgagaagtg
    15961 caccttctgc tatccgcggg tcgaggtggg catcccgacc gtgtgctccg agacctgcgt
    16021 gggccggctg cgctatatcg gcctgatgct ctatgacgcg gacgccgtcc tcgacgccgc
    16081 cagtgtcgag aacgaccagg acctcttcgc ggcccagcgc ggcgtcttcc tcgacccgga
    16141 agaccctgcg gtgcaacgag aagccgagcg cgccggcata ccgggcgact ggatcgaggc
    16201 ggcccgcaag tcgccggtca agcggctgat catggactac caggtggcat tgccgctgca
    16261 cccggaatac cgcacgatgc cgatggtctg gtacatcccg ccgctgtcgc cggtcgtcga
    16321 tgtcgtcaag gagaccggcc acgacgccga gagccaggcc aacctcttcg ccgccatcga
    16381 cactctgcgc attcccgtcg aatatctcgc caacctcttc acggccggcg atgtcgcgcc
    16441 ggtcaacgag gtcctcaaga agctcgccgc catgcgctcc tatatgcgcg atatcaacct
    16501 cggccgggac ggcaatgccg acatcccggc gaccgtcggg atgggcgagg aggagatgta
    16561 cgagatgttc cggctgctcg cgatcgcgaa gtatgccgac cgttatgtca tcccgaccgc
    16621 ccacgccgag caggcgcacg ccctcgaaga actcgcgacc gattgtgcgg tctccgaata
    16681 cggtggcggc cagtccgcgg cattcggtga gggctccggc cgcggcggcc tgacgccggt
    16741 cgccgtcgag aacctgcggg tgctcaagga gacccagacc gccgactccc tcgaggaatt
    16801 gccgggcgag aacccgctgc gcgggcgcgt caacctgctc aactgggacg gcaaggggat
    16861 gccggacggc atcttccccg gcgacaagac ggggacgccg caatgatctg gcgccggcac
    16921 cgcaagagcg ctccggcgtt gtcccgcacg gatattcgaa cgctctggca ggtcacctcg
    16981 ctgctgctcg actacccgac cgagcagctg cgggcccagg cgccgacgct gcgcgcggcc
    17041 ctcgcccaag tcccggcgga cgcgcgggcc gggctcaccg catacctcga tcatctccag
    17101 gcgacgtcgc tggcgagcct gcagagcgac tacatcgaca ccttcgacat cacccgcaag
    17161 tgcgcgctgc acctgagcta ttacacgtgc ggcgacaccc gcaagcgtgg ggtcgagttg
    17221 atccggttca agcaggccta ccgcgcggcg ggcgtcgaga tgagtggcga cgaactgccc
    17281 gaccacctgt gcgtcgtcct ggagttcggc gccgtgcacg acgtcgatat cgcctggaaa
    17341 ctgttgtgcg acaaccgggt cggcatcgaa ctgttgcacc gggcgctgcg tgccaaggac
    17401 tcggcgtggc tgccggtcgt ccagtcactg cgggcaaccc tccccgaact cgacggttcc
    17461 gacgaggaag cactcgccaa gctcatcgcg caaggcccgc ccagcgaaca ggtcggcctc
    17521 gacctccccg gctacgccat ggacccgcgc ctgaaccccc gccccctccc gatcgacttg
    17581 ggggacgcga tccccgtagg agctcaacag cgatgaacct ggccccacga agttctccgt
    17641 tccttcgtca ctctgatacg tcgcgaggtc cccgatgaac cgcttcttct tcggcatctt
    17701 cccctacctg tgcctggcgg tcttcgtcgt cggccacatc tggcgctacc gctacgacaa
    17761 gttcgggtgg accacccgca gcagccagct ctatgaggac aagctgctgc gcatcggcag
    17821 cccgctcttc cacttcggca tgctcggcgt cgtcggcggg cacatcctcg gcctgctgat
    17881 cccgcagtcg tggaccgaca agatcggcct gtcccgccac gcctaccacg tcctcgccct
    17941 ggccggcggc ataccggccg gggtcgcagc ggtggtggga ctgatcatcc tggtctatcg
    18001 gcgccgcacc accggcccgg tcttcagcgc caccaccgtc aacgacaagg tcatgtatgt
    18061 cgtgctcggt gccgtcatcg tgctcggcat ctggaacacc atcgccggca gctggctgca
    18121 gttcggcggc gagtacaact accgcgacgg cgtctccccc tggttccgct cgatcttcga
    18181 gatgcgcccc gacgtcgacc tgatggccgc tgccccctgg ggtttcaaga tccacgcggt
    18241 gctggccttc ctcctcttcg cgatgtggcc cttcacccgc ctcgtgcacg tcttcagcgc
    18301 cccggtcggc tacctcaacc gcccgtatgt cgtctatcgc agccgggacg ctcgcgcccg
    18361 cggcccgcag gtccgcaccc cgcagggcac ctgggacaag cccggtgtca agaactgaat
    18421 gtgttcctac tgcggctgcg agaacatcgc gctgatcggc cggttcatgg ccgagcatgt
    18481 cgagatcgtc aacgcgctca cggcgctgcg ggcggcgtgc cacgacgctg acgcagccgc
    18541 cgtcggcgcg gcggtcgacg cgatggaggc gctgcttcat ccgcacaccg gcgccgagga
    18601 ggtcggcctc ttcgcgcagc tgcgcctcca ggacgagttc gccgacacca tcgactcgct
    18661 gtgcgccgag cacgtcactc tggacgcgca actgatggcg atccgcgggg gcaagcacac
    18721 ggcatacgag caattcgagc acgacctgcg ccgccatatc gaccacgagg acaacggcct
    18781 cttccccgcc gccgccatcg ccatgtccgg ccccgactgg gaagaagccc tcgcccgcac
    18841 tcccggccga ggcctcgacg gacgctggga cgcgggctag gtttccaggc gcgcgtgagt
    18901 tgggactgtc gtccgccaga cggcgggcca gctttgccaa tgttgaccgg tccttatggg
    18961 caggtgctgc cagtgagcca gcccgcgctc gggacaaaac tcccatgctc aaaggggctg
    19021 ccggcggacc cgcacgggat tccggccgta tcaccgtcga tcatggctcc ccttgtccgg
    19081 acacagctga gggagagggc tcctgccatg accgaccaca ccttccgtcc cacgcgccga
    19141 atggcagcct tgctggcctg tgccgcgatc gggctgagcg cctgcggcgg cggcgaggaa
    19201 acagcctccg ccggcgcctc cggcggcggg gacgcctccg gcgtcaagat cgccgcgatc
    19261 ttctccggcc cggtcaacga cgccgactac aacgccctcg gcctgaaggc gctcgaggcc
    19321 gccaaggcga agggcgccga gacgtcatac agcgagaagg tcgccgtccc ggacgtcgag
    19381 gcgcgcctga ccgaggccgt cggtgacgga gcgacgatcg tgtggactca cggatcgcag
    19441 ttctacgaca acaccgccaa ggtcgccgcc gacaaccccg atgtcacctt catcgccgaa
    19501 tacgacggca caccgaagga ccagccggcc aacgtctgga ccatcgaccg gcagttccac
    19561 ctcggcttct acggcatggg cgtgctcgcc tccaagctga gcaagaccgg caccgtcggg
    19621 tatgtcgggg ggctctcgtt gccgttctcc ttcgccgagg tccacgcgat ggaacaggcg
    19681 atcagcgaca cgggcgccag caccaccatc aagcccgtgt ggaccggtga cttcaacgac
    19741 gccgcgaagg cccagcagtt cacccagcaa ctcctcggcc agagcgccga cgtcatcgtc
    19801 ggctccctca accagggcgc gaccggcacc ttccaggcct tcgagggcaa ggcggccggc
    19861 gacggctggg tcaccgcgaa atacaccgac aagtcggccg ccgccggcga ccactatgcc
    19921 gggtcgctcg aatacgactt cgtcgccccg ctggctgcaa tcctcgagaa gatcgccgcc
    19981 ggcaccaaga ccgggcgtta ccccctcggc ttcgacaccg gcgtgacgat cgccatgtcc
    20041 gacaaggtgc ctgcagaggc caagacggcc gtcgaggacg cgatatccaa ggtcaagtcc
    20101 ggcgcgacca cggtcaagct cgacgtcacg aagatcgggt gacccgacgg tgagcgcccc
    20161 cgacgccgcg ccgggaggcg cggcaccgcc actgctgcgc atgcgcggca tccgcaagac
    20221 gttcgggccg gtcgtggccc tcaaggacgt cgacttcacg gtcggccctc gtgaggtcca
    20281 cggtcttctc ggcggcaacg gcgccggcaa gacgacgctg atgaacgtcc tctacggcct
    20341 ctacgcccca gacgccggca ccatcgagat cgacggcgcc ccggtggtga tcgcctcccc
    20401 caaggacgcg atcgccgccg gcgtcggcat ggttcaccag accttcctcc aggtcgacgt
    20461 cttcaccgtc gccgagaacg tcgtgctcgg caccgacacc tccggggtcg acgtgaacgg
    20521 ccggatccgc gaactgtcgg agcgcttcgg cctcgccgtc gacccggccg cccgcatcgg
    20581 tgacctgccc gtcggcgtgc gccaacgcgt cgagatcctg aaagcgctct atcgcggcgc
    20641 caagatcctc gtcctcgacg agccgacgac caacctcacc ccgcaagagg tcgacgcgct
    20701 cttcgggtcg atgcgcgcca tcgtcgacga gggcatgtcg gtcgtgctga tcacccacaa
    20761 gatccgcgag acgatggccg cctgcgaccg gatgacgatc atgcgagacg gggcgtggat
    20821 cgcgacagtc gacgccgccg acacctctcc cgaagacctc gccgcccgca tggtcggcgc
    20881 cgccccggcc agcgacgaga cggccgacgt ggccctcggc ggcaccgaga tcggcgacgc
    20941 caccctcgat gtgccagagg tgaccgggcc ggtacgcgtc gtgggcagcc ggctgaccgt
    21001 gcccggcacc ggcggcaagg ccgtcgtcga ggacgtctcc atcgaactgc gcgagggcga
    21061 gatcctcggg atcgccgggg tcgccggcaa cggtcaggtc gaattggccg agaccttgac
    21121 cggctgccgc ccggcccacg gctctctcac cctcgacggc accgaactcg tcggccggcc
    21181 gaccgaagcc tggctgcgcg cgggtgtcgt ctacgtgccc gaggaccggc agcgcgacgg
    21241 catactgccc ggcgccgata tcaccgagaa cctcgtcctc ggcgcccacc ggagccgcgc
    21301 caaggccggc ctgatcgact ggggccgcgc ccgccgggag gcggtcgaag ccatctcgga
    21361 gttcgccgtc aaaaccccca gcgccgccac ggcctgcggg caactgtccg gcggcaacat
    21421 ccagcgcgtc atcctcgccc gcgccttcgc ccacaacccc aaaatgttgt tgctgcacaa
    21481 cccgactcgc ggtctcgaca tcgcctcgac ccagttcgtc tatgaccgca tccgcgccgc
    21541 ggccgcgggc gggtgctcgg tcatcgtcat ttccgaggac ctcgacgagg tcatcgccct
    21601 ggccgaccgg atccgcgtcg cctactccgg ccggctcgtc ggcgacttcg cccgcggtca
    21661 cgccgacccc tatgaactcg gccggctgat gaccggcgtt tcaggagccg catgagcacc
    21721 gtcaccgtcg ccgcccgccc cagcgtcggc gagcgcgcca aagcggcggg cgtcgccgcc
    21781 attccctaca tcctcgcggt cctcgtcgcc ttcgtcgtcg tcgcgatcat catcgccctc
    21841 ctcggccacg accccgccaa ggctttccgc gcggtcttga ccaactcctt ccgcaaccgc
    21901 accggcatgg tgcagaccct ccacaagtgg gtgccgctga ccctgctcgc cctggctttt
    21961 gccatccccc tcggcgccgg ccgcttcaac atcggtggtg agggccagat gatcctcggc
    22021 gcgaccggtg cggtcgccat gggcatcctg ctctccgatc tcccgaccgt cgtgctgctg
    22081 ccgctgctga tcgtcgccgg cgtcgccgcc ggggcgctct gggccgggat atcggcgtgg
    22141 ctgatgcaac agttcaaggt caacgagatc ctctcgacgg tgctactcaa cttcgtctcc
    22201 ttcgagatcc tcgactatct cgcctcggtc gtctggtccg acccgggcgc gggtggcgcg
    22261 gtcaccacag cgatcggcgc gggcggcgag ttgccgcgaa tcggcaagcc ccccatgcat
    22321 atcggcgtca tcctcgtcgc gctgctcgcg atcgccgccg ccttctatgc ccgccgtagc
    22381 atcgccggat tcgagttgcg cgcggtcggc ctgaacgagc gggcggcgaa gctgcacggc
    22441 atacgcaccg gcaaggtggc agtcatgtcg atgatcgtcg ccggcgccct ggcggggctg
    22501 gccggcgcga tcgaggtcgc cggcgtgcac catcggatgc tcgagggcat ccagtccaac
    22561 ttcctcatcc tcggcatcat catcggcttg atcgcccgcg gctccctgac cgcggtgccc
    22621 ttcgtcgcgt tcggcatcgc ggtgctcgag gtgggcgcgg gaagtatgca gcggacctcc
    22681 ggcgcccccg tcgagatggt gctcatcctc gaagggctga tcctgctctt cctcctgatg
    22741 tcggactggg tcaccgaccg attgcgcctg cgcgcgtcac gaaaggccgc ggcatgagcg
    22801 acttcatcac cttgaccatc accgcgatgg tgcccttcct cttcgtcgcc cagggcacga
    22861 tgctctccgg ccgggccggc atcttcaacg tctcgcaaga gggcctgatg ctgctcggcg
    22921 ccgccatcgg ctacctcgtc agcctcaagt ggggcggcaa tctcgtcggc ctgctcgtcg
    22981 ccgccctcgc cggcgcggcc ttcggcgcca tcctggggtg ggccacgacc tacctgcgga
    23041 tggaccagtt cgtcgtcggc ctcgccctgt tcttcctcag cggcggcacc gccagcctgc
    23101 tctatcgcgt cgtgatcggc gccgacgagc cgccgcgcat ccgcccgctg cccgacatcg
    23161 tcttcggcca ggacgtgctg gtcttcgtcg cgatcgggtt gtcggcgctg ttgtggtggt
    23221 ggctctatcg caccgattcc ggtatgcggt tgcgctcggt cggtgagaac cccaaggccg
    23281 ccgactccct cggcatcaat gtcacccgca cccggatgct cgccgcctcc ctcggcgggg
    23341 ggctgatggc cgtcggcggc gcctatctgc cgatggcgtt ctccggcgtg tatgccgatg
    23401 gcatcgtcgc cggccgcggc tggctggcca tcgccctggc cttcttcggc gggtggcgcc
    23461 cgcaactcat cctcgccgga tcgctgttct tcgccgccat ggacgtgctc gcccagcgcg
    23521 cccaggtgag cgggatcggc ataccgcacc agttcgtctc gatgctgccg tatgtcgcga
    23581 ccctgctggt gatgatcttc gccttccggt gggccctggt cccgcagttc ctcggcaaga
    23641 actacgaccg cgagtcgcgg atcaccggct gaaccgcctt ctcccccaag gaatctctgc
    23701 tagcagacgt tagtgaaatc cgcacaccta ggacggtgtg cggatttcac tagcctctgc
    23761 tgagttggcg cgcagccctg cttgggtatg ccgcgggcga gcgtcagccc gccagcgccg
    23821 aggcggccgt atgcggcagc tcctggccga aggcgccgcg ctggtagtcc tcgaaggcct
    23881 gcatgacctc ggacttggtg ttcatgacga acggaccggc ccaggcgatg ggctcgcgga
    23941 tcggcttgcc gccgaggagg atcacctcga ggccacgctg accctgcccg tcggcggccg
    24001 cgccggcggc cacgcgcagc gcgtcgccgc ggccgaggac ggcggcctgc cccgcgcgga
    24061 tgggggtgct gtgggggccg accacgccga caccgctcat cacatagacg agggcgttga
    24121 agtcgcgccg ccacggcagc gccagctcgg cgcccggcgc gacggtggcg tggatcatcg
    24181 tcatcggcgt gtgcgtggag cctgggccct ggtggccggc cacatcaccg gcgatgagcc
    24241 ggatgatgct cgccccgtct tcggaggaca gcagggcggt ggactcggcg cgcaggtcct
    24301 gatagcgcgg agtgacgagc ttgtcggcgc gcggcaggtt cacccacagc tgcaggccgt
    24361 ggaacaggcc gccgctcatg acgagcgatt cgggcggggt ctcgatgtgc agcaaaccgg
    24421 acccggcggt catccactgg gtgtcgccat tggtgatggt ccctccgcca ccgtggctgt
    24481 cctggtgcac gaaggtgccg tccatgatgt aggtgacggt ctcgaagccg cggtgcgggt
    24541 gccacggggt gcccttgggc tcccccggcg cgtagttcac ctcgcccatc tcgtccatca
    24601 tgatgaacgg atcgaggtgc gccaggtcga tgccggcaaa agcccgcttg accgggaagc
    24661 cctcgccctc gtaaccggtc ggggcgctgg tgacgcttag cacgggtcgc gagacggcct
    24721 ccggggcgac cggggcgacg cggggcagca cgctgaagtc agcggcggtg atggctggca
    24781 tgaggacctc ctgatcgttt cgttttcaac tatatcgtcg aaacgccgcc gtgccaagat
    24841 ccattccccg ggaaaagccg gcgccgccgc accctccccg gtcgggaagg cacggcggcg
    24901 ccagtggtgc agctgccagc cgtccgcttg tggcgggcgg gacaggtcgt gcggtgggtg
    24961 gttcgccgtc aggcgagcgc gtcgatgtct gccttgatcg cctcgatctt gtcggcaccg
    25021 atcatcgcac cggccatgcc gatggcctgt gcggcggaca tgccgcggac catgccgatc
    25081 atggggttgg acgtgacgcc gggggcgtgc ttctccatga tggccgcgac ctcggggtcg
    25141 tcgagcagag cgcccatggt ggtggtgttg atgtcgtaac gagccatgcg gtgggttctc
    25201 cttcgggtac cagccagtaa ctctgcggtc agcctatgtc acggcgggcg gtatgccgtc
    25261 gccgcaccgc gcgagacaag ggtcactgca cggcgtgggc gcgcttcacc cgtcgccgcg
    25321 gcggaagccg gcgcgctgag ccgactcttc gtccatgaac cagacgtccg ggaccgtcat
    25381 gtcgtaccag tccgagccgg gcacctggta gatgccgagc gagtacaccc ccttgatggg
    25441 gtgaccgagc ggcatacaac cgtcctcgag cggagccgca gacccgacgc tccagccgcc
    25501 gtcgaccacg ggatgatcgt ggacgatgac ctcgtggctg ccggcatcgc cagagtcctc
    25561 gggcgcaccc tgggagtgga acggatcggg ctgtgagaca tagctgcgcg ccgggctgac
    25621 cgcagcggcg ccccgggctc cagccacgcc ggcggccgcc gggccggccc agtcgggccg
    25681 gtcggcctcg ggctcctcgc cgtagacctc ggcgcccgtg gccaggtgct cctcggctgc
    25741 ggcgttggca gcatcgactg cgctggcccg ctcgccttcg aggctcaggt cggcctgcag
    25801 gtcgccgcct tgcgcgtcga acccggcgga ttcgccgagg ccgaagcggt gcgacgccga
    25861 gggctcggag acggaggcat cgcccggggc atcggcggcg gcgtcggcgt catcgcccga
    25921 ccacgcggcg gcgatctcct cggcctcggc ctcctccggt gcggggatga ccggcatcgg
    25981 cgcctcggcg gactcggcct gcatgccgat gtcgctgctc ggggtgccgg cgtcgtccgc
    26041 cgcccaggcg tcggcaatgt cctcggcatc cgtggtgtcc tgcgccggga cgggggcggc
    26101 gttctgcggt tcggccgcct cgtcgtcgag gccggcggcg tcgacatagg gaccgtcggc
    26161 gccgtcgccg gccacgtcga agccggtggc gtccacctcg ggcaacgagc cggcctcgtc
    26221 gaggccggcg gcgtcgatct cggcgtgggc cgccgcggtg gactcgctgt ccatgccggc
    26281 ggcatcgacg tgaccggcag ccgattcgga cgcgtcgacg tcaggtgcgt cgaacggcgc
    26341 agctgctccg gctgcgaccg cggcatccgc tgcggcgcca tcgggggtca tgccgggaag
    26401 cgtgccgtcc tcgggcaggt cgaagtcgtc ggcgtcggcg ctcaggtcgg gcaggtccag
    26461 gctcggcatc tcggtgtccg ccgtcgcggc gcgggcctcg gcctcggcag ccgcgaggcc
    26521 gtccacctcg gcatcccccg cgagacggga accggggatg accccgtcct cgcccaccac
    26581 gaagtccggg tccgtctcga ggtccgggtc cgccacgagc tccggcgcgc tgcccggctc
    26641 ggggtcggca tcggcatccg acgcctcgac gtcagcctgc gcactcgtca gcgtcgcctc
    26701 cgagccgcgg tcggggtggg tctcttccca gacatggggg gtgcggccgg cggccacgtc
    26761 ggcctcggcc tcggcggcct cgaagtcgcc gacgagcgcc tcggccccct cgttggcgtc
    26821 ctcgccgcgc gcggcggacc aggcggccgc gcccatggtg cccgcaccca cggccccggc
    26881 agcgccggcg gccacggccg cgtccgcggc ggactcgcgg cccagcccgt cgacaccgcc
    26941 caagtcatgg cgtgactcct cacgggtcac gatcgtctcg ggccggcggc gcgaggtgag
    27001 caacagccag aggatcaccc ccagcaggag cagaaagatc agcagcaaga tgagattgcg
    27061 gccttcgggc attgagactc ctgtctggac ccggcgtgcg gctcgcgcgc gacggaagcg
    27121 gcggaatggg gcgaaccatc cagtcatgca agcgagccta ttcttcccca gatcgcccgt
    27181 ggggcgagag gccccgccgg ctagaccgtc ccgaggccga gttcctccgt ggaacgggcg
    27241 cgcatctcga ccttgcggat cttgcccgtc acggtcatcg ggaactcctc gacgcacatg
    27301 acgtagcgcg ggatcttgta gtgcgcgaga ttgcccgtgc agaacgctcg cacggtctcg
    27361 gcggtcagct gttcggcgcc ggcgcgcatc tggatccagg cgcacaactc ctcgccgtac
    27421 ttcgcgtcgg gcaccccgac cacttgcgca tcgacgacgt cagggtgggt gtagaggaac
    27481 tcctcgatct cacgcgggta gatgttctcg ccgccgcgga tcaccatgtc cttgatccgg
    27541 ccggtgatgc ggacataccc gtcctcgtcc atcacggcca ggtcaccggt cttcatccat
    27601 ccgtccacga cggcctcggc cgtcttggcc tcctcctgcc agtagccgag catcaccgaa
    27661 taccccttgg tgcagaactc gcccgtgacg ccccgcggca gagtttcgcc cgtcagggca
    27721 tcggcgaccc ggatctccag atggggcccg acgcgcccca ccgtgccgac cttgtgatcg
    27781 aaggagtcgt cggtgcgggt ctgggtggag acgggggagg tctccgtcat cccgtaacag
    27841 atggtcatct cctcgatgcc ggcggcgatg accttcttca tcatctccgc cgggcacggc
    27901 gacccggcca tgatgccggt gcgcacgctg ctgacgtcat aggactcgaa gtcgggcagc
    27961 gcccactcgg cgatgaacat cgtcggcacg ccatagagcg aggtgcactt ctcctgctgc
    28021 accgccgcca gggtcgcggc cgggtcgaag gcgggtgcgg ggatgatcat cgcggcgccg
    28081 tgagtggtcg cggccaggtt gcccatgacc atgccgaagc agtgatagaa gggcaccggt
    28141 atgcagatcc gatcctcctc ggtatagcgg cagatctcgc cgacgaagta gccgttgttg
    28201 aggatgttga agtgcgacag cgtcgcaccc tttgggaatc ctgttgtgcc agaggtgtat
    28261 tggatgttga tgggttcgtc gcacgtgagg ctgtccgaga tggcctggac ttcctcggtg
    28321 ctcacctcgt ccgccctgga gagcaggcgg tcccaggatt cggtgccgaa gaagacgaca
    28381 tcgcgcaggt cggggcattt gcctcggacc tgttcgacca tcgcctggta gtcgctcgtc
    28441 ttgaaggccg gcgtcgcgac gaggaggctg atgcccgcct gccgcaggac gtactcgagt
    28501 tcgtgcgtgc ggtatgccgg gttgatgttc accaggatca ggccgacgcg ggccgtggcg
    28561 tactggacaa ccacccactc cgcgctgttc ggggcccaga tcccgacccg ctcacccacg
    28621 gcatacccgg ccgcccgcag ggcccgggcg acccggtcga cctccgcgcc gaactcggca
    28681 tacgtcatcc ggatcccggc ggcgacgtcg acgaccgcgt cgcggtcggg aaagcgcgca
    28741 atcgtctccg ccaggttggc gtcgatggtg gtgcgcagca gcgggggtgt ttcctcgccc
    28801 ttggcgtggg agagagtggg agcgtccatc gaatcctcct ggcgtcgtcg cgatctccac
    28861 tattgcgcgc ctgccggcgc gccgctaccc aggggtcaac aagcggtcgt tccggggcgc
    28921 cgagtggcca tcagaggaca gctcccgggt tcatgatccc gagggggtcg atggcggcct
    28981 tgatccggcg ggagaggtcc atgacgtccg ggccgagctg ggcggggagg gcggccttct
    29041 tgagccggcc gacgccgtgc tcgccggtga tcgtgccgcc caactcgatc gccgcggcca
    29101 tgacgtcggc gaacgcggcc tcggcgcgct cgagagcgcc aggctcggcc gggtcgtaga
    29161 tgacattggg gtgggtattg ccgtcgcccg catgcgcgac gaccggtatg ggcagtccgt
    29221 gccggaggga gatctcctcg atccgcgcga gcagcgccgg cagtctcggg accgggaccc
    29281 cgacgtcctc cagcagcatc gagcccttgc gctccagggc gggcagggag gagcggcggg
    29341 cggcgaggaa catctcgccc tcgtcgggat cggtcgtcga gaagacctca ctcgcgccgg
    29401 caacttggca aatctcttcc acgacaagga cttccgcggc acgggcagcg ccgggcgagt
    29461 cgcactggac gagcagcaga gcggccgcgt cgcgatcgag acccatctcg cgcaagtcct
    29521 cgaccgcgtt gatcgagtag ttgtccatga attccacgag cgacgggcga agccgattac
    29581 ccagtgcgac aacggcgttg gccgcggcag cgacgctcgg aaaagtggcg acgagggtcg
    29641 cggcggggct gggcgcgggg atcagccgca gcgtcccacc gacgatgacg ccgagggtgc
    29701 cctcactccc gacgaagagt ttgagcagcg acagaccggc gacgtccttg atcgtgctgc
    29761 cgccgagctt gaccaacgtc ccgtccggga ggacgacgtc gagcccgagg acgtagtcgg
    29821 tcgtcacgcc atacttcaca cagcacaggc cgcccgcgtt ggtcgcgaga ttccctccga
    29881 tagaacagat ttcgtatgac gacgggtccg gcgggtagtg caggccgagt tctcgcgccg
    29941 cctgcttgac ggtgatattc aaggccccgg gctcgacgac cgcgacccgc atcgcgggat
    30001 cgatctccac tcgcgtcatg cgatccaggc tgagggtgat ggcgccgtcg acggcgagcg
    30061 acccgccgga cagacccgac ccggcaccac gcggcacgat gccgatgcgg cgctgcgccg
    30121 cccagcggac cacggcctgg acctcctcga cggtgcccgg gcgcaccacg gccaatggcg
    30181 tccccgcccc cgcgcccttg gaccagtcgt ggcgataggc cgccatccgg tcggggtcgg
    30241 cgatgacgaa ctgcggggcg agcaactgcg taaggtcggc cagcacgtcc gcgccgtctg
    30301 gctccggcag gccgtccggc gacagtgggc gttccgaagt catggtctct gtatagcaag
    30361 gtgcccggtc cacccgctcc cggacgccga tatcgagtcg tgaccaaccg gcggaccggg
    30421 gccgccgtca tacgggtgtc aacgccggct gggcccggct cgctcactgc cgcaagggcg
    30481 ctctccggtg ccctcatcac ccaaatgtcg atgctggccg agatgcaggc ggcgggcggc
    30541 ccccattgga acttcgaacg ggagctgaac cgagccgtcg gtgccgcgct gcccggcgag
    30601 ccgtacgcga cgatcacccg acaggccatc tcgtcctatc cctccaagga cggcctgcgg
    30661 gtcgcggtcg accaatgcgc cgcatacgcg tgcgccgcgg gcgtcgtcaa cgccaccatc
    30721 ccctgggacc gcctgatcgc cccggatgcg gatatgtcca accagtgggg ccactgacgc
    30781 tccgacgggc cggcgatcgg ctcccctcgc actcgacgac cttgaagtgc actctcgacg
    30841 ccgtcacgtg gggcgggtca gacggtcgat gaaggccagg agtagggcct cgcgcaggcg
    30901 gggcggcgcc tgcgccaaca tggcgttgat ctccgccatc cggtcgggca gggcgcagcc
    30961 accgccgagg ccggccagtg cgagtatgcc gtcgaaggtc gcctcgtcga gggcggcggg
    31021 gtcgaggtgc gcgagcaccc cggcgatcgc cgggtcgacc gacacgggcc cagcggctcg
    31081 ggcggcggcg gcgctctcgg cgaggacctc gagcagttct tcgacgcggg gcgccgtcgc
    31141 cgcgcacaga ctcaggtgca ggctcggcgg gccgccgcgg aagggcagtt gcgggctgag
    31201 cagccacccg cgggaggacg cctcgtcggc gagggtgaac acgtcatacc cggcgtcctg
    31261 ggtcgtcgtg accgccagca gcgtgctgtc gggagcgacg agcgggcgca gcccgaggcc
    31321 gcgttccgcg atctgctcgg ccaagcccac cgtcgcctcg cgggcgacct gggcgagttc
    31381 gcgatagccg tcgaggccga gatagcgcac gacggcccac gccgccgcga gcgggccacc
    31441 cgatttcgtc gattgcatcg tcgtgttcag catcgtgtag cccggccagt ccgccgccgc
    31501 gaagaaatgg ctctttcgca aagatgcgtt gcggtgcaac aggatcgaga cacccttggg
    31561 cgtgtagccg tatttgtgca ggtccaccga gatgctgctc acgccctcga tctcgaagga
    31621 ccacggcggc gcaccgtcga ggaagggcag cacccacccg ccgatgcagg cgtcgacgtg
    31681 cagccgtatg ccgtgctccc ccgccacgtc ggccagcgcc gtgatcgggt cgatcacccc
    31741 gtgggcgtag gagggtgccg agcccacgag gagcaccgtg gtcccgtcga ccgccgccgc
    31801 catcgcggcc gggtccgcgc ggaaggtcac cgggtcgacg ttgacgaaga ccggacgcac
    31861 gccgaagtag tgcgccgcct tgtggaaggc cgcgtggacg gtggtcggca gcaccattga
    31921 cggcgaatcg cgctgggctg cagagttttt cgggtcgtcg cgggccgcga ggacagccag
    31981 caggatcgac tccgtgccgc ctgacgtcac cgtcccggcg aacccgtcag gagcccgcag
    32041 cagatgtccg gcgagccgca ccaggtcgtt ctccatcgcc agcagcgacg ggaaggctgt
    32101 cggatcgaga ccgttggtgc cggcgaacga cgcgagcgcg cggcggccga tctcgtcggc
    32161 gtcggccagc cccgagtcgt agacatagga cagcgtccgc ccgccgtgcg tggcaacatc
    32221 gttggcgcgc aacgcctcca gcgccgtgag gatgtcgtcg gcgcgcgtca tacgattcct
    32281 tcctcggatg cggcccgcac ggccgactcg tcaagacgat agcgagcgag cgccagcaac
    32341 gacagtgcga tgacgacggc cggcacgagc gagaacccga gttggatcgc gtcgatcgcc
    32401 cccgccggct ggcgcatcga atcacccgag gacgagacgt agccgcccag ggcgaggacg
    32461 gcggcataca gcccggggcc gagcgcgagg ccgagggttt cggcgcccgt ccagaccccg
    32521 gtgaagacgc cgacccgatt ctcgcccgtc cgcgcggcgt cgacggccgc cacgtcgggc
    32581 agcatggcga gcgggaagag ttgcgtgccg gcatacccga tcccgacgac cgccacggcg
    32641 gcggcgagga gggccaaaga cccttctcgc acaacgaaaa tcgccagagc cccggtgagc
    32701 aggaccagcg acgcgaggag gaagccgccc ttcttgccca accggtcgcc gacggcctgc
    32761 cacagcggca tcacgacgag cgccggcgcg acgaagcaga cgaagatgac cgtcgcggcg
    32821 ccgcccccgc cgacgaccca ccgggcgacg tagtcgacgc cggcgagcat gcagccggtc
    32881 gccaaggcct gcaggacgta tgccgtcagc aaccgccgga agtccggcgt cgccgcgacg
    32941 agccgcagtt gctcccccag gcccgcgacg ggggcggcga cgtcgtgcag gcgcacgccc
    33001 gcggtgccgc gccatgcggc atacgcaccg gcggcgatga ggagggcgac gaagatgccc
    33061 accgcccgat agccccacgc accgccgacg gcgtcccgga tcgccggcga cagcccgccg
    33121 ctgagcagga tggcgagcgc gaggatcgcc acccgccacg tcatcaggcg agtccgttcg
    33181 gcgtagtcgg aggtcagctc ggccggcatc gcgacatagg gcacctggaa gaaggcgtat
    33241 gccgtcgcgc aggcgaggaa ggccagcgcg acccaaccgc cgccgagcca ggtcggcgcg
    33301 ctcgggcccg cgaagagcag gacgaagcag ccggccagga ccagcccggc acgcagcaga
    33361 aagggccggc ggcggccgtc ggggtgggcc gatcggtcgc tgaggcggcc ggcgatgggg
    33421 ttgaggacga cgtcccacgc cttgggcagc aacacgatca ggcccgcgat cgccgcgccg
    33481 acgccgatgt ggtcggtcag gtagggcagc aggagcaggc cggggacggt gccgaacgac
    33541 ccggtggcga cgccgccgag gccatagccg atgcgcaccc ggcgcggcag ggcgtccggc
    33601 gcaacgctgc gggactgggt cacgcgggca aatctaggcc ttggtgggca gggcgcatag
    33661 ggccaatggc ggcccggcgc aaccgctgtt cggctgcgcg acgtgccggc ccccgtccta
    33721 ggcaactggc gagtaagtta gcgctgtgac gcttccgtcg gtcgctgccg accccgacgc
    33781 caccgccggc gccgccgcgg tggcggctgc agcgcgggcc ggggtgagga cgatgttcac
    33841 cctctccggg gcgcacgtct ttccgctgta tgacgccgcc caccgaggcg cggccccgtt
    33901 gcggatgatc gacgtgcgcc acgaagcgag tgccgtcttc gccgccgaag cggtcggaaa
    33961 gctcacgcgc acaccgggtt tcgctgtcgt caccgcaggt ccgggagtga ccaacgctgt
    34021 cagcccggtg acacaggcac acttcagcgg tgctcccgtg gtcgtcttcg gcggccgggc
    34081 gccggaactg acctggggtc gcggcgcttt gcaggagatg gatcaggcgc cgctcttcac
    34141 cacgatcacc aagagcagcg cgaccgcgca cactcctgat gaggtgggtg ccctcacggc
    34201 ggcggccttc acggcggcga gcacggcgca tcgcgggccg gccttcgtcg aggccgggat
    34261 cgacgtgctg ttcgcgcccg ccggtgacgt gggtactgcc gtacccgggc ccggcggccg
    34321 cccggcgccc gacgaggagt ccgttgccga gatcgcgcgc ctgctccgcg aagcacggcg
    34381 gcccatcgtc atcctcggca gcgacgtgtg ggcagatggc gcggagcagg ccgcgctcga
    34441 cttcgtcacg gcactcgacc tgcccgcgat cggcaacggg atgggccgtg gcatcctccc
    34501 gccgacccac cccggctgcg tgacccgggc ccggcgggcc gcgttgacac agtgcgatgt
    34561 ggcgatcgtc gtcggcgccc cgctcgactt ccggatcggc tacggcacct tcgggccggc
    34621 cggcacggac acgcccgtca tccacattgc cgacagcacc gaccagatcg cgacccaccg
    34681 cacactggcg gcgagcgcgg cgggcgacct gggcggcata ctgcggacgc tctgcgacct
    34741 cgtcggcggc cccggtgggt ggcgcgagtg gcgcagcggc ctgcgcgagc aggcggacgc
    34801 ggccggcgcg gccgacgccc cgctcctgac cgccggaggc gacctgatcc accccgctcg
    34861 gatctacggc gagttgcttc cccgcctcgg cgacgatgcg atcgtcatcg gcgacggcgg
    34921 cgatttcgtt tcctgggcag ggcgattcgt ccaacccgcc cggccgggac gctggctgga
    34981 cccgggcccg ttcggctgcc tcggcgccgg tctcggcgcg gcgatcggtg cccgtgcggc
    35041 cgacccggac gcgccgatcg tgttgctcct cggtgacggt gcggcgggga tgtcgctgat
    35101 ggatctcgac actctcgtgc gccacgacct gccggtcgtc atcgtcgtcg gcaacaactc
    35161 ggcgtggggc ctggagaagg gcccgatgca gctgctctac ggctacgaca tcctcgccga
    35221 cctggccccg gggacccgct acgacaaggt ggcgagcgct ctcggcgggg cgagcgaact
    35281 cgtcgacaga cccggcgaca tcggcccggc gctcgatcgc gccctggcca gcggcacgcc
    35341 atacctgctg aacgtcctga ccgacccggc cgccgcctat ccccgctcca cgaccggcct
    35401 gtagcgcccg ctcggtgggt gtgtgaccgg acgcgcagca aaatgggttt cgccccggtg
    35461 gagggcggca ggcagaatcg ccgcatgaag ccgatcgtcc agagccgcaa gctgcagggt
    35521 gtccgttatg acgtgcgagg gccgatcctc gtcgaggccc agcggctcga ggccgaggga
    35581 cacaagatcc tcaagctcaa catcggcaat accgccccct tcggcttcga ggcacccgag
    35641 gcgatcctcg ccgacatgat ccacaacctg cccgaggcgc agggctacag cgactctcgc
    35701 ggcatctact ccgcccgcac ggcggtggcg cagtactacc agtccaaggg gttcaaggac
    35761 actctcgtcg acgacgtctt catcggaaac ggtgtctccg aactgatttc gatggtcttg
    35821 acggcattca tcgacgacgg caacgagatc ctcgtcccgg cgcccgacta cccgttgtgg
    35881 acgggagcgg tcaccctcgc gggcggcata ccggtgcact atcgctgcga cgaggacaac
    35941 gagtggaatc ccgatctggc ggacatcgag tccaagatca cggagaacac gcacgcgatc
    36001 gtcatcatca accccaacaa cccgaccggc gcggtctaca gcgaggggat cgtgcgcggc
    36061 ctggtcgaca tcgctcgccg gcacgacctc gtcgtcatgg cggacgagat ctacgagaag
    36121 atcatcttcg acgacgccgt ccaccaccac gcggcgacct tctgcggcga cgacgtgctc
    36181 tgcctgacct tctccgggct gtccaaggcg tatcgggtat gcggttatcg cgccgggtgg
    36241 gtgatggtca gcggcccgaa gcacatggcg accgacttcc tcgagggcct gacgctgctg
    36301 gcgaacatgc gcatgtgcgc gaatgtgcct gcgcagcatg cgatccagac cgcgctcggg
    36361 ggctaccagt cgatcacgga gctcgtcgtg cccggcgggc gcttctacga acagatcaaa
    36421 ctggcgagca agttgctcaa cgacatcccc ggcgtctcgt gtgtcgaacc caagggcgcg
    36481 ctctattgct tcccgcggct ggatccgaag gtctatccga tcgaggacga ccagcagttc
    36541 gtcatcgatc tgttgcgcgc caagaagatc ctggtcacgc acggcacggg gttcaactgg
    36601 ttcgagccgg accacttccg gctcgtgacc ctcccggaca ccgaggtgct caccgaggcc
    36661 atccaccgca tcgccgcctt cctcgagacc cagcgggccg gctgacgcgc tccgcatcgg
    36721 tcaaggggcg tggctcgcga ggtgccgact aagctgggcc gcgttatgag cactcgtccc
    36781 ggccccgaga gccgcgcggt gaacacgtcc ggcaaatgga tcctgaccgc cttctgcctc
    36841 gccgcgctcg ccgtcttcgc actgctcgtc ccctccgtcc gggagtggct cgctcgcgac
    36901 gacgactccc agctgtccgc gatcgaactc accatctccc ccgcgacgag caaggaggtc
    36961 gatgcgtgcg caccggtcag ccaggacgcc ctcgaccggg ccacgacggc cgtgacgggg
    37021 acctatgagg ggatcagcga cggcgcggcg acattcgtcg ccgatcactg gttccggggc
    37081 gggcccgccg agcgggtggt catcagctcc agttcgagcg cgaacctcga ccgcgcgctg
    37141 gagcaggcgc agctcaacac cggccgcgtg ctgatcgcct cccgctccgg cgaggtgctg
    37201 ctctgcggcg cgtccggccc gtacacctcg gaactggcgt cgttctacgt gcagacgtat
    37261 gagcagcgca ccgcctcgcc gacctcctga cccgagacgc gtacgcgatc tgcgtgcccc
    37321 gcggcacgaa tcggcccggc acgtgtcggg ccggcacgt
//
LOCUS       DAOPJG010000011        34441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1330_length_34441_cov_7.723172, whole genome shotgun sequence.
ACCESSION   DAOPJG010000011 DAOPJG010000000
VERSION     DAOPJG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 34441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34441
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1330_length_34441_cov_7.723172"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1107)
                     /locus_tag="PKU75_02295"
     CDS             complement(172..1107)
                     /locus_tag="PKU75_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPF79735.1"
                     /translation="MHPLVRTAGSLAAAGVAGLGYASLIERNAFVLRRFTMPILPAGA
                     DPIRIVQMSDMHLVPRQTRKIEWVRRLADLEPDLVVNTGDNLAHLQAVPAAVRALEPL
                     YGIPGVFALGSNDYYAPKVKNPARYLRKDSRVALDEERTLPVGELIDSFTREAGWLDL
                     NNARGKLHVAGLDIEFVGVDDPHLDRDRYAEVEGERAAGSDVFLGVCHAPYTRVLDAM
                     ARDGVDAIIAGHTHGGQLALPLYGALVTNCDLDRSRAKGLGRWWPGANGIPSAQAPDD
                     AAWLHVSAGLGTSPYAPVRFACRPEASLITLTPRT"
     gene            1168..1623
                     /locus_tag="PKU75_02300"
     CDS             1168..1623
                     /locus_tag="PKU75_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPF79736.1"
                     /translation="MTVSKASLQSDLTDAMRAQDKVRAGTLRMALTAITMEESAGAHH
                     ELSDDEVLKVLAKEAKKRREAATAYTDAGRPELAEVENAELAILQAYLPEQLDEAAVA
                     GIIEEVIAETGATGMAQMGPVMKAVQAKVAGRADGKAVAAAVRAALAKS"
     gene            complement(1674..4169)
                     /locus_tag="PKU75_02305"
     CDS             complement(1674..4169)
                     /locus_tag="PKU75_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPF79737.1"
                     /translation="MPVRPDRASMPHLMSLVAAFLAVSVAMGLLAAGLLMPAVGAMGG
                     SSNKAIDAFENIDKTFTTTALSQQSEIRDANGKLLATPYDQNRIVVPLSKVAPIMQKA
                     QIAIEDSRFYEHGALDVRGTARAFFNNAAGNATQGGSSLTQQYVKLTLQAEALKRGDE
                     AAAEEAVDVNYGRKLREMALAMQVEKQMTKKQILAGYLNLAYYGDQAYGVEAAARHYF
                     NVKAKELNLTQAATLAGVVQSPGSSDPHNNPEAAQKRRDIVLDRMAALGIITAKEAAS
                     AKKPSVKSLLRIRDSEGGTCDRSVEPYFCNYVIAYLKTLKVLGKNVDERTLAINRGGL
                     RIKTTLRSDWNQQILKDLRSRVPAGDPSGVGAAAAVIEPGTGKVLAMAQTSEYGLSKD
                     KKSTVGRTTQSWVVPTAYGGTLGFPIGSTAKVFTLLTALEAGIPVNGSVPAPHAGVGK
                     TNAHIFPNDVYIDECRTYQQGGWSVNNDYQTTGETQTFRKATADSINTAFAATMIKLG
                     GCKVRDMWNRLSAFDGNGQEISPDPSNLVLGSGSMPPLTLANVYATLAANGKWCPVSP
                     IESITTSDGKKLKLPALKCKQVLDKDIVAGADELLKGVITGGTGTTGIRSQAGFFKTA
                     AGKTGTHEGHQQSWFVGYTTRLALAVYVGTPIRQLEMDGITIGGRYYGNVFGGTIAAP
                     LWGIIMKQITADMPDEAFPAPSSKVLYGIQTPVPGVIGMATANAKATLEAAGFTVAVG
                     SPVSSTMPAGTIAAQSPQGSAAQGSIISISPSTGYVPPPVVHQPPPNTNTPSTNSSTK
                     PPGNTSTTPATNTSTKPSGNTVTKPPGNTGTKG"
     gene            4325..4606
                     /locus_tag="PKU75_02310"
     CDS             4325..4606
                     /locus_tag="PKU75_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016663301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPF79738.1"
                     /translation="MSVLAQIEAGHGPQNWAAFGACAQADPDVLFVRGKAQHEAKQVC
                     RECPVALQCLADALDNRVEFGVWGGLTERERRAILRAHPDVTSWQAVLL"
     gene            complement(4619..5809)
                     /locus_tag="PKU75_02315"
     CDS             complement(4619..5809)
                     /locus_tag="PKU75_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HPF79739.1"
                     /translation="MARATTQAGSTKPGSTRPAGPALDIDALLADPGKRIIVCCGSGG
                     VGKTTTAAALGLRAAESGRRVVVLTIDPALRLAQSLGLTALDNTPRPVSGIDTSAGGS
                     MDAMMLDMKRTFDEVVEQHATPEKAQQILSNPFYVAVSSSFAGTQEYMAMEKLGQLRA
                     QSAADARWDLIVVDTPPSRSALDFLDAPKRLGSFLDGRFIRILMAPAKAGGRAYLKAA
                     TATISLAMTVMTRVLGGELLRDVQTFVAALDTMFGGFRERADQTYALLQDDETAFVVV
                     ASPDRDALREAAFFVERLATERMPLAGVVVNRVQMVAGALSRGRAEDAADALDAGSAA
                     SSASADLLRLHADLATSADRHQGAIARFRTANPGVALTQVPTAARDIHDVEGLRTVGA
                     DLAG"
     gene            complement(5814..6821)
                     /locus_tag="PKU75_02320"
     CDS             complement(5814..6821)
                     /locus_tag="PKU75_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPF79740.1"
                     /translation="MSDYPGAQGVRLHIVTGKGGTGKTTVAAALGIALAGQGRKVLLA
                     EVEDRQGISQTFDVPPLSHTETLIFDDPSGGSLWGMAVEPKSALLEYLHKFYKLGRAG
                     SLLEKIGAIDFATTIAPGLRDVLLIGKVYEAVGRREGRGRHGKTPTYDAVVLDAPPTG
                     RVARFLGVNEEVAEVARMGPIKGQADSITAMLKSRITAVHIVTLLEEMPVQECLDAVH
                     DLTANGLPVGAIIVNQVRDPILEDKDLEALSGGASARLRADVAADLAGAGVPDPKGMT
                     DVLLDEAAAHLERLDLQGEQDQRIQEQGRAVFYLPALADGLEAGGIRVLADELHVQGM
                     V"
     gene            6874..7029
                     /locus_tag="PKU75_02325"
     CDS             6874..7029
                     /locus_tag="PKU75_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HPF79741.1"
                     /translation="MTRWEYATVPLIVHATKQILDQWGSDGWELVQVVPGPDGNGLVA
                     YLKRPLD"
     gene            7122..7592
                     /locus_tag="PKU75_02330"
     CDS             7122..7592
                     /locus_tag="PKU75_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPF79742.1"
                     /translation="MSAVEERLAELGLTVPDVVPPVASYVPAVRQGDLVFTSGQLPMV
                     AGSMPATGKVGEGEGLVSPEQAKELAQACALNAIAAVKALIGDLDQVTRVVKVVGFVA
                     SDPAFTGQPVVVNGASDLLGKAFGDAGIHARSAVGVVALPLDAPVEVEIIVSLA"
     gene            7614..8429
                     /locus_tag="PKU75_02335"
     CDS             7614..8429
                     /locus_tag="PKU75_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF79743.1"
                     /translation="MRDFPFPVSAATAAHFEAWTAAPGEPPPPKLAATVLLLRDADAG
                     AVEVFMQRRVASMAFAARKAVFPGGSVDPGDREELPWSGPPAADWAAALGIDRADAAA
                     LIAAAIRELFEEAGVLLAGPAGGGDGRPAAEWLAQERGRILAREATIASVLRDGDLVA
                     RTDLLGFHAHWITPEVEPRRYDTFFFTALCPPGQHADDQTPEAESAEWIDPAAMLRDE
                     RPALMPPTIVCLEDVAAATSAAGLVRMRRDVQVIQPVPVRHGDGWAMRMQVRP"
     gene            8426..9229
                     /locus_tag="PKU75_02340"
     CDS             8426..9229
                     /locus_tag="PKU75_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF79744.1"
                     /translation="MTTPDPRGVLPAWSGGEITTHATCVLAPNPGPMTLDGTNTWVVM
                     APGAGEAMVIDPGPLDEDHLQAVCRVVERRGARVTETILTHAHLDHSEGAARFAELTG
                     CRVRPVGPGHDDLADGSVVSAGGVALRVVATPGHTADSLSFVLEADHALLTGDTILGR
                     GTTVVAHPDGELAAYLDSLERIRALTGGGEVVTILPGHGPVIPDAHGLVGYYRTHRRE
                     RLDQVRQALADGATDVEGVLRRVYADVPEAVWPAARLSIQAQLDYLAAR"
     gene            complement(9269..9949)
                     /locus_tag="PKU75_02345"
     CDS             complement(9269..9949)
                     /locus_tag="PKU75_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPF79745.1"
                     /translation="MDLDVLRRSPLFAGLDEQATSDLLSSMIPIRMERGDILFNEGDQ
                     GDRLYVIAEGKVKLGRTSYDGRENLLAILGPGEMFGELSLFDPGPRTATATAVAETQL
                     VGLGTDALHAYLSARPQVSFTLLAALARRLRRTNESIADLVFTDVPGRVAKALLELSQ
                     RFGRPVEEGVLVAHDLTQEELAQLVGASRETVNKALADFATRGWIRLEARAVLLMNVE
                     RLHKRAGV"
     gene            complement(10017..10469)
                     /locus_tag="PKU75_02350"
     CDS             complement(10017..10469)
                     /locus_tag="PKU75_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79746.1"
                     /translation="MEWLALAVLALRGAVVVRRGGSRGRGAADPFGADSGEVADRLAR
                     IVADAGSTQRRLLSDRLASLRSHRVPLRAIHHSPVPKVARLLFADGTVILARSLVQGE
                     VTRLGLMREAGSVVIADFGSHPDGIEVALSGVRGDACRVLAVGLDQSD"
     gene            10590..11273
                     /gene="nth"
                     /locus_tag="PKU75_02355"
     CDS             10590..11273
                     /gene="nth"
                     /locus_tag="PKU75_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPF79747.1"
                     /translation="MYRELADFYPYAHCELDFESPLQLLVATILSAQTTDVGVNKVTP
                     VLFARYPDAAALAGADRTEMEEIVKPTGFFRAKTDALIKLGAALEERYGGVVPGRLKD
                     LVTLPGVGRKTANVVLGNAFGVPGITVDTHFGRLVRRFGWTEEEDPVKVETAVGALIP
                     RKDWTMLSHVLIFHGRRICHAKRPACGACPVAKLCPSFGIGETDPGKALTLLKYELAP
                     GRVAAEANR"
     gene            11270..11914
                     /locus_tag="PKU75_02360"
     CDS             11270..11914
                     /locus_tag="PKU75_02360"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPF79748.1"
                     /translation="MSEAPAWLRGIPQRVAELDPAYFRDFTPPPDATRRSAVLMLFGP
                     DPDGGEDVLLTQRGTNLRSHAGQVSFPGGRVDPQDAGAVGAALREAEEEVGLDRAGVE
                     VIDTLPPLYLSPSANVVTPILGWWHSPSEVWAASEIEVERVVRVKVEDLVNPANRFLV
                     DTPVGYVSPGWEVHGLFVWGFTAKLLDVTLQLAGLMEPWDEAVRRPLPPHLWRR"
     gene            complement(11931..12377)
                     /locus_tag="PKU75_02365"
     CDS             complement(11931..12377)
                     /locus_tag="PKU75_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPF79749.1"
                     /translation="MGSTRYDAVENRPVDEAGRLLDTDGSRTIGQLVADASREMSTLV
                     RSEIALAKAEVTGGLKSGGKGAGLLGAAAFLGLLGLIFLFHTLARVLDIWLPISASYG
                     IVTALLFLVAAVLGLMGKKAISQAKPVPEQAITEAKETVEAVKSSV"
     gene            complement(12451..13779)
                     /gene="nhaA"
                     /locus_tag="PKU75_02370"
     CDS             complement(12451..13779)
                     /gene="nhaA"
                     /locus_tag="PKU75_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPF79750.1"
                     /translation="MTIPPVRGRIFSRPSWPEATALKAALRTETVGGALLLLAALLAI
                     AWANSPWHESYAALRDLSVGVDWGHLKLTLGHWAADGLLAIFFFVAGLELKREFLVGD
                     LRDPRRAAVPIVAAVCGVIVPAATYLIVTRATGGTGDALRGWAIPSATDIAFALAVLA
                     VLGSHLPAALRAFLLTLAVVDDLIAIVIIAIGYTSDFAGMPLLLAVAPLAAFTFLVQR
                     RITYPLLLVPLALLTWGLVHASGVHATVAGVLLGLVVPVRPRKGRMPAVSVHGPKTDV
                     AHVFEHRLRPLSAALAVPVFAFFAAGVRVVGGGFGEAISDPVALGVMAGLVIGKVVGV
                     FGGTYLTARFTKADLDPGLGWADVVGLSFLTGIGFTVSLLIGELAFGAGSDRDEHVKL
                     AVLLGSLAAAALAALVLVPRNARYRAAEAREEQDDDGDGIPDVYEYEQTR"
     gene            14036..15493
                     /locus_tag="PKU75_02375"
     CDS             14036..15493
                     /locus_tag="PKU75_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF79751.1"
                     /translation="MTTGDERTALLDQLKQLWELVGGHEVRAGGPALVEELTGHFGSA
                     PHEMAVLTEEVANHRFVDFDIALEVVAARDAGHRAIGIGGGESRYHHNFSDMIQPPGV
                     GFGGGRFPLAQVDYANVATGPDTERAAIAFGIRLFRYAGVPVAVLLRKANPYRGRGSA
                     TLEVLCPDQPTAARLLDELREISLQRSVLRGQVVGLANSGYEQAEEGLTFIARPNLTA
                     AQVILPEGVLERVVDHVVGIAAHAEMLAAHGQHLKRGVLLFGPPGSGKTHTVRHLLSI
                     TPGHTVVLLAGDTLRFVGLAAKLARAMQPAIVVLEDCDLIAEDRDMHHGAKPLLFEVL
                     DAMDGLDADADVTFLLTTNRVEAMERALAQRPGRVDLAVEVPLPDEAGRLALLRLYAP
                     VGAFGEEVLAETAARTAGTTASFSKELIRRAVLAAAKEGLEPSDAHLRGANDVILADG
                     ERLSRALLGVQDSGVPGNGADPRATPDGSGSFHLI"
     gene            complement(15501..16394)
                     /locus_tag="PKU75_02380"
     CDS             complement(15501..16394)
                     /locus_tag="PKU75_02380"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HPF79752.1"
                     /translation="MFEEGQLYSPVTVGADGSVEVHLGADHPGLNDPAYLARRGEIAA
                     AALAWEPGTPVPLIGYTAEENEVWRIAGAELAPKHQRHAHSEYLRGKAALGLPADRVP
                     QLAMVTEHLFPLTGWSYAAAPGLVPLREFYGALGERVFHSTQYLRHPSQPLYTPEPDV
                     IHEVIGHANQLASPRFARLTEAAGAATRRLETDAAVKAVADVFWFSMEFGVIWEEGIL
                     KAYGAGILSSFGEMDAYSHMEIRPLDLHQMATIPYDITTYQSVLFAAENLDQLEDVVG
                     GFFDTVDDVSAHRLLGEHAHA"
     gene            complement(16577..18532)
                     /gene="acs"
                     /locus_tag="PKU75_02385"
     CDS             complement(16577..18532)
                     /gene="acs"
                     /locus_tag="PKU75_02385"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPF79753.1"
                     /translation="MSASHPTFPPDAAFAAQANGTAEMYDAAAADHEGFWAEQARTRI
                     TWAKDFEQTLDWSDAPFAKWYAGGELNAAYNCVDRHVEEGRGDKVAIHFEGEPGDTRT
                     ITYADLQREVCKAANALEALGLGKGDTVAIYLPMIPEAAIAMLACARIGAPHSVVFGG
                     FSAEALNTRINDAACKLVITADGGYRRGAPSALKPAVDAALEHAETTVEKVLVVKRTG
                     QDTPWTEGRDIWWSDVVDEASETHEAQPHDSEHPLFILYTSGTTGKPKGIFHTTGGYL
                     TQCAYTNAVVHDVHRDTDVYWCTADIGWVTGHSYIVYGPLANGATQVMYEGTPDTPHQ
                     GRWWEIVEKYKVTILYTAPTAVRTFMKWGHEIPEKFDMSSLRLLGSVGEPINPEAWLW
                     YRKWIGGDRCPIVDTWWQTETGAIMISPLPGVTTLEPGSAQKPIPGISAEILDDEGRP
                     FTEAEKVGYLVLTKPWPAMLRGIWGDPERYKQTYWSRFGPKYYFAGDGAKYDEKGNIW
                     LLGRVDDVMNVSGHRLSTAEIESALVSHPKVAEAAVVGANDETTGQAVVAFVILREEH
                     IEEADEVGEGGDLVKELRDHVAKEIGPIAKPRTIMIVPELPKTRSGKIMRRLLKDVAE
                     GREIGDVTTLADSSVMNSIAQGLGSGG"
     assembly_gap    18671..18770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18830..19216
                     /locus_tag="PKU75_02390"
     CDS             18830..19216
                     /locus_tag="PKU75_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79754.1"
                     /translation="MDPRRTRHVRHWHKYTTQALPEDLWFTFTEPSGKVIGHASTIAE
                     FVRGVRSVDAGVIWWHLSRGDFSRWLAISLGDPDPAALAATTERDAIARHAADIHSAR
                     RRLLALIEDLYRLDPAPEADAEPEPA"
     gene            complement(19316..20986)
                     /locus_tag="PKU75_02395"
     CDS             complement(19316..20986)
                     /locus_tag="PKU75_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HPF79755.1"
                     /translation="MAALTSLAAATTVGNAKLNIAIFVIFVLVTLTVVVKVASGKKTA
                     EQYYTAGGAFSGRQNGIAIAGDYLSAASFLGIAGAIALQGMDGFLYSIGFLVAWLVAL
                     LLVAELMRNTGRFTMADVLSYRLKQRPVRIATSVSVLCVSLFYLLAQMAGAGGLVSLL
                     LGVSGETAQNLVIAVVGAVMVTYVLIGGMKGTTWVQIIKAVLLISAAAIMTVWVLGKY
                     GFSLSGLFTDSVDVAKTSTNPKTAAGAENLLHYGTKYGVTTLAKIDFISLSMALVLGT
                     AGLPHVLQRFYTVPTSKAARKSVEWAIWLIGGFYLLTLVIGFGAGALVGPDAINAAPG
                     KQNSAAPLLAFELGGSWMLGIVSGIAFATILAVVAGLTITASATFAHDLYKEVFKRGQ
                     STQEQEVRIARISAIAIGLVAIFGGMLAKSQNIAFLVALAFAVAASANLPTILYSLFW
                     KRFNTRGALWSIYGGLIASIGLIIFSPVVSGKAADPKTGLSPSMVTNPDIDFHWFPLD
                     NPGLVSIPLAFFLGWLGSVTSKEFDAEKYAEMEVRSLTGHGVGKALEH"
     gene            complement(20990..21325)
                     /locus_tag="PKU75_02400"
     CDS             complement(20990..21325)
                     /locus_tag="PKU75_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HPF79756.1"
                     /translation="MSAQQPTTSEAYQAVQSSEEFQTLRKKFRGFVFPVTAFFLVWYF
                     LYVLLSIFAQDFMNRFLFGKITIGLVFGFLQFLTTFAITFIYARWANRELDPLADAIG
                     SRLDGVKEH"
     gene            complement(21520..23016)
                     /locus_tag="PKU75_02405"
     CDS             complement(21520..23016)
                     /locus_tag="PKU75_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HPF79757.1"
                     /translation="MRPASLAAIALVCVLTVTVGTLGLRMSRATSDFYVAGRTVTPRR
                     NASAIAGEYLSAASFLGVAGLMYQRGIDILWFPVGWTVGYLVLLVLVAAPLRRSGAYT
                     LSDFAELRLESRQIRGFASLLTVGIGFLYLLPQFQGAGLALRQATGWPAWIGALLVSV
                     VVLAAVAVGGMRSITLVQAMQYWIKLTAIAIPAFVLLAIWSQHSSGPVELGDWAVPST
                     DPAGLYTTYSTMLALCLGTMGLPHVLVRFYTNPDGRDARRTTVGVLALLGGFYIFPPV
                     YAVLGHIHLPHLAPGTRVDTMALQLPEAMTDGRSATLLTAVLAGGAFAAFLSTASGLV
                     ISVAGVVDQDVLRPLVARRTGEDAHNSVSYRMAATVAVIVPALLSRVVEPLGLAATVT
                     LAFAVAASTFAPLLVLGVWWHRLTTTGALAGLAVGTLSALVTLGAAFLHVEPHGWPGL
                     LLAQPAAWSVPLAFVTMVVVSRLTPGKVPPHTNRTLSRLHTPESLLPP"
     gene            complement(23016..23399)
                     /locus_tag="PKU75_02410"
     CDS             complement(23016..23399)
                     /locus_tag="PKU75_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79758.1"
                     /translation="MSPAQRPPSERVRVTASRRSAPRHTARPVADELSDQTVLGEIYV
                     RGLMRAQLRLALSLLLLAVLLLGGVPVLFAMLPQTRTVTLGPLPLPWVTLGLVVYPAI
                     VISARYYVRASERLEAAFVDVVGRG"
     gene            complement(23396..24136)
                     /locus_tag="PKU75_02415"
     CDS             complement(23396..24136)
                     /locus_tag="PKU75_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPF79759.1"
                     /translation="MISSPALVALVVDDEAPARSELRYLLEQDERIAHVLVAASGTDA
                     LRVLEDEKVDVVFSDISMPGLDGMELARVLARFATRPAVVFVTAHDQHAVDAFAIDAI
                     DYVMKPVRPERLAEAVRRVAAAAISAPAEPQDDDETIPVELGGVTTFVSRKDIRYVQA
                     HGDYARLHTEIGSHLVRISLNTLEERWAAAGFVRIHRSTLVSLKHVTQVRMDRGRATV
                     RVGEADLQVSRRHMRDLRQQLLARPSAP"
     gene            complement(24133..25353)
                     /locus_tag="PKU75_02420"
     CDS             complement(24133..25353)
                     /locus_tag="PKU75_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPF79760.1"
                     /translation="MRGFDLPTAMVTVLIVFLIWLGIRLVVTVRGRSRGFLSDANRAT
                     YETLHTAARAARHLGSGLTPAGGAKAARHLRTLLDTPALSLIDQSGTVTWEGAHNHHA
                     GAVAKLAEPVLRDGHTAVLDHGEVACTDVDCAIRAAVIAPIVCEGRVVGALAAYGPHA
                     NAGLARATAEVAGWLSAQVELAELSKERSRATEAELAALRAQISPHFIYNSLGAIASF
                     VRTDPARARDLLLEFADFTRYALRKGGAFTTLADELRNVERYLVLEQARFGERLQVSL
                     LIAPEVLSVTVPYLAIQPLVENAVRHGIANLEGAHTVSITAADRGAEAEIVVEDDGVG
                     ADPAHIQAVLDGTADTDSHGLGNVDARLRTIYGDERGLVVETAPGAGMRVSFRVPKFL
                     PAAITAGDNRLAGS"
     assembly_gap    25437..25536
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25899..26756
                     /locus_tag="PKU75_02425"
     CDS             25899..26756
                     /locus_tag="PKU75_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPF79761.1"
                     /translation="MSDALAALQSLAADERVTAASDRAREACTQLRWHQALRRRIPEA
                     AAESRVRGAWASGELDGARSSIEIVRDLMRGAREPSPDPDPAERVLHGAIAATAATEG
                     LVASVRSAPRQAYARLHLAAASHLVDSGDLGRPRTSAECAEFSLLGPAPDAAEAAARL
                     FALDDVIAADDLPTLLVVAIAHAEIVHARPFARGNGLVARALNRVLLQACGLDPTGVV
                     VPEAGHGAQGGVAYHGALQAYGSGSLAGLMLWIDHCADAVVAGAREGGEIADAVLAGR
                     FSATPTLPG"
     gene            26824..27588
                     /locus_tag="PKU75_02430"
     CDS             26824..27588
                     /locus_tag="PKU75_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79762.1"
                     /translation="MTILGVVSGSGGAGCSTLAAALGVQAAALGGTAVVVDGDPVCGG
                     LQVTTGVEHLPGHRWAELAEVDGVVDGSRLRERLPQADGCAVLSGGRVSSTLTALSPE
                     PVPPTAFDAVLDGLERSCDLVVVDWGRQLRPGQGASLYLLPLTARGIADAQAWLAQFD
                     AEQLAGVVTRSRRGRRKLAEAAAAGLGLPLLGEVVDDRRVAVAERRGGMPGAGRGPLA
                     RLARNIVGAVVQAEQARTGRADRVPRGRRRVATGRA"
     gene            27588..28613
                     /locus_tag="PKU75_02435"
     CDS             27588..28613
                     /locus_tag="PKU75_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HPF79763.1"
                     /translation="MRFDDGIDAAVRAGRVPDRRSIRAVVDTAAERLGARGVTRTEAV
                     LEAELAGLGPLAGLARAAGVTDILVNGDAQTWADRGAGLERVPEHDFESPEHVRRFAV
                     RLAGVAGRRLDDAQPWVDGLLPGAVRLHAILPPLSSGGTCISLRMLRRDAVRLTDLER
                     VEMCTPDQGARLRAMIANRDAFVVTGGTGAGKTTLLAALLGEVDPSDRILVVEDVLEI
                     PLNSGHVVRLQARPPNVEGQGSVTLVDLVRQALRMRPDRVVVGEVRGAEVRELLQALN
                     TGHEGGCATMHANKPADVPARLEALGALAGMSPAAVRAQVATALKTVVHVARTPVGRR
                     ITAIEDL"
     gene            28617..29735
                     /locus_tag="PKU75_02440"
     CDS             28617..29735
                     /locus_tag="PKU75_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPF79764.1"
                     /translation="MGRILAALLLLLGGYAVWPRRRGMRRRREAATSDTRVDSLAAVL
                     ESVALCLQAGLTPVQAVDVAVQQRRDSAAEQDDAIGSVLAEVQRALHRGAPGGPAWAR
                     HSDQIPELRVVAGAWTLTEASGSSLHPAVMWSVAQLREKRAAKERLSAVTAGTTSSMM
                     LMLALPLAGVPIGLLVGVPPGELYGSFPAGVALVVGLSLAASGAVMSKRLLRKALQPK
                     KISAEEGLPDATLDDVADAALMLQLALSSGAGIVESIEQVAVVSPHRIRTELRRVVAG
                     YRWGLGHRTAWSYADVSWEPVSAALALSLEHGAAPAASVKAAGQRLAESEKSRLQAAA
                     GRAETFLLIPLVVFFLPAFALTTVIPLVIALVPRGGLF"
     gene            30008..30226
                     /locus_tag="PKU75_02445"
     CDS             30008..30226
                     /locus_tag="PKU75_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HPF79765.1"
                     /translation="MSHKLAARFTATKATLLDRATAKNDQEGLTTIEWALVTLMVCAF
                     AVTALGILSSFGVAEIGKLIKNAFGGKA"
     gene            30351..30677
                     /locus_tag="PKU75_02450"
     CDS             30351..30677
                     /locus_tag="PKU75_02450"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HPF79766.1"
                     /translation="MTIELAFGMIVFALLLLLTLTAIATGLDHIRVSEASRVGARLAA
                     RSEPVSHVIAAAKDQAPDGSVVSVTGDGGSVVVTVRAPERAPFARLGWDVAAVARSVA
                     PRELRP"
     gene            30674..31084
                     /locus_tag="PKU75_02455"
     CDS             30674..31084
                     /locus_tag="PKU75_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79767.1"
                     /translation="MTARWSPSPRRPSPAPSRHHEAGSGTILTIVLVAALIILLPATL
                     GLVTAVQAKQRARAAADLGAIAAVSNFMAGLDAATSCRRAAGFVTANAAQPTGCYITP
                     EGAATVTARVTARLPVVGMRTSTTSARAGPVRQR"
     gene            complement(31330..31752)
                     /locus_tag="PKU75_02460"
     CDS             complement(31330..31752)
                     /locus_tag="PKU75_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79768.1"
                     /translation="MIVLGLLFILLAALAGTWLYLALMDMVGDHTVDFTALGVTIGFS
                     PMALLLIGFGLACALGIGLYLIRAGISAGARRRRERKELEREAAENQRASEAAAAERL
                     RAERAAADERGRSITDPVNRTDSVNRNDAGWTPGDGRR"
     gene            complement(32027..34375)
                     /locus_tag="PKU75_02465"
     CDS             complement(32027..34375)
                     /locus_tag="PKU75_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HPF79769.1"
                     /translation="MTSAPHDTAGHAPIAHSGQAPVDILSTLSGVAPHGPLLHVRSFP
                     SRTGATSAMPEWINPALAAALTGAGIDALWSHQSLAAELVRSGRHTVLATGTASGKSL
                     AYLLPGLTAVLDGTRAPNGRGATMLYLAPTKALAADQADHITRLALPGVRAATYDGDT
                     APEERRWIRDHANIVLTNPDLVHHSLLPGHAGWASFLRALSIVVIDESHAYRGIFGSH
                     VALVLRRLRRVLERYRSAPVFVFASATAGEPGAHASDLVGLPVAEVTHDGSPRGALTF
                     ALWEPQEYVDEHGRPHRPAASTESARLLSRLVGGGVQTLAFARSRAGVEAVAAAANRS
                     LAAAESSHRVAAYRGGYLPEERRALERDLRAGTLRGLAATNALELGIDIHGLDAVVMA
                     GWPGRLSSLWQQAGRAGRRGSAALAVLVAADDPLDAYLMKHPEAIFGEPVERTVVNPG
                     NPRLLPPHLIAAAYERRITEADEGVFGPGMWPALDELVAAGQLRRRPGGWFWPQDTRP
                     DAVGSLRTAGEVVAIVEGASGRIIGTIDSASADLQVHPGAVHVHQGQTYVVTHLDLER
                     ASATVAAGDPGWSTQAQSRSEFTFATPDAAQLSGSLTVCRGQVTVHTQVVSFMRRLPG
                     GEIIGRHELDLPVREFVTEGVWWILPADLVAEALPAQVVPGALHAAEHAAIGMLPLFA
                     TCDRWDIGGVSSACHPATGEPTILVYDGYAGGAGYAARGFEILPQWLSATRALIAECP
                     CQRGCPRCVVSPKCGSGNEPLDKPGAITVLDLVLGELARGPG"
BASE COUNT         5007 a        11709 c        12504 g         5021 t
ORIGIN      
        1 gcgacctctt cgtcccgaac gaagcgcgct accaagctgc gccacacccc gtggctgtcc
       61 gcgactcgcg tcacggatcg tcgccacccg ctggatggcg acgccacagg ataaccccag
      121 cggggcggcg ggcagaaatc ggttccgcct gtggcggatc gtccgctgct gtcaggtgcg
      181 cggggtcagg gtgatcaggc tcgcctcggg gcggcaggcg aagcgcacgg gggcatacgg
      241 cgaggtgccc aggccggcgg agacgtgcag ccaggcggcg tcgtcgggtg cctgcgccga
      301 cggtatgccg ttcgcccccg gccaccagcg cccgagcccc ttcgcgcggg accggtccag
      361 atcgcagttg gtgaccaggg cgccatacag ggggagcgcg agctgcccgc cgtgcgtgtg
      421 gccggcgatg atggcgtcca ccccgtctcg cgccattgcg tcgaggaccc gcgtgtacgg
      481 cgcgtggcac accccgagga agacatcgct gccggccgcc cgctcaccct cgacctcggc
      541 ataccgatcg cggtccaggt gcgggtcgtc gacgccgacg aactcgatgt cgagcccggc
      601 cacgtgcagc ttgccgcggg cgttgttgag gtcgagccag ccggcctccc gggtgaagga
      661 gtcgatcaac tcgccgacgg gcagtgtccg ctcctcgtcg agggcgacgc gggaatcctt
      721 gcgcagatag cgcgcgggat tcttcacctt gggcgcgtag tagtcgttgg agccgagggc
      781 gaagacgccg ggtatgccgt agagcggctc gagcgcccgc acggcggcgg gcacggcctg
      841 caggtgggcg aggttgtcgc cggtgttgac gacgaggtcg ggttcgaggt cggcgagccg
      901 gcgcacccac tcgatcttgc gggtctggcg gggcaccagg tgcatgtccg acatctgcac
      961 gatccggatc gggtcggcgc cggcgggcag gatcggcatc gtgaagcgcc gcaggacgaa
     1021 ggcgttgcgc tcgatgaggg aggcgtagcc gagtccggcg actcccgctg cggcgagcga
     1081 gccggcggtc cggaccagag ggtgcatcgc tccagtctcc cagaggtcac gcgaaatcgt
     1141 gttgcagcgg attgggacaa tgggtgcatg accgtttcca aggcttcgct gcagtccgac
     1201 ctcaccgacg ccatgcgcgc gcaggacaag gtgcgcgccg gcaccctgcg gatggcgctg
     1261 accgcgatca cgatggagga gtcggcgggt gcgcaccacg agctgagcga cgacgaggtg
     1321 ctcaaggtgc tggccaagga ggccaagaag cggcgcgaag cggcgacggc ctacaccgat
     1381 gccgggcgcc ccgaactcgc cgaggtggag aacgccgagc tggcgattct gcaggcctac
     1441 ctgcccgaac aactcgacga ggcggccgtg gccggcatca tcgaggaggt gatcgccgag
     1501 acgggtgcca ccggtatggc gcagatgggc ccggtcatga aggccgtcca ggccaaggtg
     1561 gccggccgcg cggacggcaa ggcggtcgcc gcggcggtgc gggcggcgct ggccaagagt
     1621 taggtaagac gatctggcgg tcatgacagc cagatcgtct tacctaaagt ccgctacccc
     1681 ttggtgccgg tgttgccggg cggtttggtg acggtgttgc cgctcggttt ggtggacgtg
     1741 ttggtcgccg gcgtcgtgct ggtgttgccg ggcggtttcg tgctcgaatt cgtgctcggc
     1801 gtgttggtgt tcgggggcgg ctggtggacg acggggggcg gcacatagcc cgtcgacggc
     1861 gagatgctga tgatgctgcc ctgggcggcg ctgccttggg gtgactgcgc agcgatggtt
     1921 ccggcgggca tcgtgctcga cacgggagac ccgacggcga cagtgaaacc cgctgcttcg
     1981 agcgtcgcct tggcgttggc cgtcgccatc ccgatcacgc ccgggacggg agtttgaatg
     2041 ccgtagagga ccttcgaact cggggcaggg aaggcttcat cgggcatgtc cgcggtgatc
     2101 tgcttcatga tgatgcccca gagcggggcg gcaatcgtcc caccgaacac gttgccgtag
     2161 tagcgcccgc cgatcgtgat gccgtccatc tcgagttgcc tgatcggggt ccccacgtag
     2221 accgcgaggg ccagacgcgt ggtgtagccg acgaaccagg actgctggtg gccttcgtgc
     2281 gtgccggtct tgccggccgc ggtcttgaag aatcccgcct gacttcgaat gccggtggtt
     2341 cccgtgccgc cggtgatcac gcccttgagc agttcatcgg ctccggcgac gatgtccttg
     2401 tccaggacct gcttgcactt cagtgcgggc agcttcagct tcttgccgtc ggaggtcgtg
     2461 atcgattcga tggggctgac ggggcaccat ttgccgttcg ccgccagcgt cgcgtacaca
     2521 tttgccaggg tcaggggagg catcgagccg gacccgagga cgaggttgga cggatccggt
     2581 gagatctcct ggccgttgcc gtcgaaggcc gagagccggt tccacatgtc ccggaccttg
     2641 cagccgccga gcttgatcat cgttgccgcg aaggcggtgt tgatcgagtc tgccgtcgcc
     2701 ttgcggaacg tctgggtctc gccggtggtc tggtagtcgt tgttgaccga ccagccgccc
     2761 tgctgataag tgcggcattc gtcgatgtag acgtcgttgg ggaagatgtg cgcgttcgtc
     2821 ttgcccactc ccgcgtgggg cgcgggcacc gaaccgttga ccgggatgcc ggcctcgagc
     2881 gcggtcagca gcgtgaagac cttcgcggtc gacccgatcg ggaagccgag cgttcccccg
     2941 tatgccgtgg gcaccaccca gctctgggtg gtcctgccca cggtcgactt cttgtccttg
     3001 ctcagcccgt attcggacgt ctgcgccatc gccagcacct tgcccgtgcc cggctcgatg
     3061 acggcagccg cggctccgac gccggacggg tcgccggcgg gcaccctgga ccgaaggtcc
     3121 ttgaggatct gctggttcca gtcgctgcgc agggtcgtct tgatccgcag gccaccccga
     3181 ttgatcgcca gggtgcgctc gtcgacattc ttgccgagca ccttgagggt cttcagatag
     3241 gcgatgacgt agttgcagaa gtagggctcg acgctgcggt cgcaggtgcc gccctcggag
     3301 tcgcggatcc gcagcaggct cttgacggac ggcttcttgg cgctggccgc ctccttggcg
     3361 gtgatgatgc caagggcagc catccggtcg agcacgatgt cgcggcgctt ctgggcggcc
     3421 tcggggttgt tgtgcgggtc ggaactgccg ggactctgca cgacaccggc cagcgtcgcg
     3481 gcttgcgtga ggttcagctc cttggccttg acgttgaaat agtgccgagc cgccgcctcc
     3541 acgccatagg cctggtcgcc gtaatacgcc agattcagat agccggccag gatctgcttc
     3601 ttggtcatct gcttctcgac ctgcatggcc agcgccatct cgcgcagctt gcggccgtag
     3661 ttgacgtcga cagcctcttc ggcagccgct tcgtcgccgc gcttgagggc ctcggcctgc
     3721 agggtcagct tgacgtattg ctgggtcagc gacgagccgc cctgggtcgc attgccggcc
     3781 gcgttgttga agaacgcgcg cgccgtgccg cgcacatcga gcgcgccgtg ctcgtagaag
     3841 cggctgtcct cgatcgcgat ctgcgccttt tgcatgatgg gcgccacctt gctcagcggc
     3901 accacgatgc ggttctggtc gtagggcgtg gcaagcagtt tgccgttggc gtcgcggatc
     3961 tccgactgct gcgagagcgc agtggtcgtg aaggtcttgt cgatattctc gaaggcgtcg
     4021 atcgccttgt tgctcgaacc gcccatcgcc cccacggcag gcatgagcag gcccgcagcg
     4081 agcaggccca tggccacgct caccgcgagg aaggcggcca ccaaggacat caggtgtggc
     4141 atcgacgcgc gatccgggcg gacaggcatg aggttcaggg taactgccgg acctgacaac
     4201 gccgttcacg ccggcacgcg cgcccgaccg gagttcgtca ggaaagaatc tccaatccgg
     4261 atgtatcagc acgtgcccaa ccccctcagt cccccgtagc gggtaaagag aatgggagtt
     4321 gtggatgagc gttctggcgc agatcgaggc cggccacggc ccgcagaact gggccgcatt
     4381 cggcgcgtgc gcccaggccg acccggacgt gctcttcgtg cggggcaagg cccaacacga
     4441 ggcgaaacag gtatgccgcg agtgcccggt cgcgctgcaa tgtctcgccg acgcgctgga
     4501 caaccgggtc gagttcggcg tctggggcgg gctgaccgag cgcgagcgcc gcgccatcct
     4561 gcgcgcccac cccgatgtca cctcctggca ggcggtgctc ctctgaggcg aacgccggtc
     4621 aacccgcgag atcggcgccg acggtgcgca agccttcgac gtcgtggatg tcgcgggcag
     4681 cggtgggcac ctgcgtcagc gcgaccccgg gattcgccgt ccggaaccgg gcgatcgcgc
     4741 cctggtgccg gtcggcgctc gtggcgaggt cggcgtgcag gcgcagcagg tcggcgctcg
     4801 cgctggacgc cgccgacccg gcgtcaaggg cgtcggccgc gtcctcggcc cgcccgcgcg
     4861 acagcgcccc ggcgaccatc tggacgcggt tgacgacgac cccggcgagc ggcatccgct
     4921 ccgtcgccag ccgttcgacg aagaacgcgg cctcccgcag cgcatcccgg tcgggggagg
     4981 cgacgacgac gaacgcggtc tcgtcgtcct gcaacagggc atacgtctgg tcggcgcgct
     5041 cgcggaagcc gccgaacatc gtgtccagcg cggcgacgaa ggtctgcaca tcacgcaaca
     5101 gttctccacc gagaacgcgc gtcatcacag tcatcgccag cgagatggtg gccgtcgctg
     5161 ctttgaggta tgcccgcccg cccgccttcg ccggcgccat caggatgcgg atgaagcggc
     5221 cgtcgaggaa ggacccgagg cgcttggggg cgtcgaggaa gtccagggcg gagcgcgacg
     5281 gcggggtgtc gacgacgatc aggtcccacc gggcgtccgc cgcgctctgc gcgcgcagct
     5341 gcccgagctt ctccatcgcc atgtattcct gcgtgcccgc gaaggaggag gagaccgcga
     5401 catagaacgg gttggacagg atctgctgag ccttctccgg ggtcgcgtgc tgctcgacga
     5461 cctcgtcgaa ggtccgcttc atgtcgagca tcatcgcgtc catcgagccg ccggccgagg
     5521 tgtcgatgcc ggacaccggc cggggcgtgt tgtccagcgc cgtcagcccg agggattggg
     5581 ccagccgcag cgccgggtcg atcgtcagga cgacgacgcg gcgcccggac tcggccgccc
     5641 gcagcccgag cgcggccgcg gtcgtcgtct tgccgacgcc gccggagccg cagcagacga
     5701 tgatccgctt gcccggatcg gcgagcaacg cgtcgatgtc cagcgccggc ccggccggtc
     5761 tcgtcgagcc cggtttcgtc gagcccgctt gcgtcgtggc acgcgccatg tcgtcacacc
     5821 atcccttgca cgtgcagttc gtcggcgagc acccgtatgc cgcccgcctc cagcccgtcc
     5881 gccagcgccg ggaggtagaa gacggcccgg ccctgctcct gaatccgctg gtcctgttcc
     5941 ccttggaggt cgaggcgttc gaggtgggcc gccgcctcgt ccaggaggac gtcggtcatg
     6001 cccttggggt cgggcacgcc ggcgccggcg aggtcggcgg cgacgtcggc gcgcagccgg
     6061 gcgctggccc cgccggagag ggcctcgagg tccttgtcct ccaggatcgg gtcgcgcacc
     6121 tggttgacga tgatcgcgcc gaccggcagg ccgttggcgg tcaggtcgtg gacggcgtcg
     6181 aggcactcct ggaccggcat ctcctcgagg agggtgacga tgtgcacggc ggtgatgcgg
     6241 ctcttgagca tcgcggtgat cgagtcggcc tgccccttga tcggccccat ccgggccacc
     6301 tcggcgacct cctcgttgac cccgaggaag cgggccaccc gcccggtggg cggggcgtcg
     6361 aggactaccg cgtcatacgt cggggtcttg ccgtgccgac cgcggccctc gcggcgaccc
     6421 accgcctcat acaccttgcc gatcagcagc acatcgcgca gcccaggcgc gatggtggtg
     6481 gcgaagtcga tcgcgccgat cttctccagc agcgacccgg cccggcccag cttgtagaac
     6541 ttgtgcaggt attcgagcag cgccgacttg ggttcgacgg ccatccccca caggctcccg
     6601 cccgacgggt cgtcgaagat cagcgtctcg gtgtgcgaca gcgggggcac atcgaaggtc
     6661 tggctgatgc cctggcggtc ctcgacctcg gcgagcagca ccttgcggcc ctgacccgcc
     6721 agggcgatgc cgagcgcggc cgcgaccgtg gtcttcccgg tccccccttt gccggtcacg
     6781 atatgcagtc gaacgccctg agcgccgggg taatcactca ccccgaaacc atacgtcggc
     6841 ccggtgtcgg caaccggcga tagggtcggg tccatgactc gatgggagta tgcaacggtc
     6901 ccgctgatcg tccacgccac caagcagatc ctcgaccagt ggggctccga cggctgggaa
     6961 ctggtccagg tcgtgcccgg cccggacggc aacggcctgg tcgcctatct caaacggccg
     7021 ctcgactgaa cccacacaac gccttcgctc ctgcgtcgct ccgctatgtc gcggggatcc
     7081 cggcgcaagc ggactgaacc ctacaacccg gaaggtatgc cgtgtctgct gtagaagaac
     7141 gtctcgccga actcggactc accgtccccg acgtcgtgcc gccggtcgcg tcgtacgtcc
     7201 cggccgtgcg tcagggcgac ctcgtcttca cctccgggca gctgccgatg gtggccgggt
     7261 cgatgcccgc cacgggcaag gtcggcgagg gcgaggggct ggtcagcccc gagcaggcca
     7321 aggaactcgc ccaggcgtgc gccctgaacg ccatcgcggc cgtcaaggcc ctgatcggcg
     7381 atctcgacca ggtcacccgc gtcgtcaagg tcgtcgggtt cgtcgcgagc gaccccgcgt
     7441 tcaccgggca gccggtcgtc gtcaacggcg caagtgactt gctgggcaag gcattcggcg
     7501 acgcgggcat ccacgcccgc tcggccgtcg gtgtcgtcgc gctgccgctc gacgctcccg
     7561 tcgaggtcga gatcatcgtc tcgctcgcct gatcccgacg accacgcgcc cacatgcgtg
     7621 acttcccctt ccccgtgagc gccgcgaccg ccgcgcactt cgaggcatgg acggcggcgc
     7681 cgggggagcc gccgccgccc aagctcgccg cgacggtcct gctgctgcgc gacgcagatg
     7741 ccggcgcggt cgaggtgttc atgcagcgcc gggtcgccag catggcgttc gcggcccgca
     7801 aggccgtctt ccccggcggc agcgtcgacc cgggcgaccg ggaggagttg ccgtggtccg
     7861 ggccgccggc cgctgactgg gcggccgcgc tggggatcga tcgggccgat gctgccgccc
     7921 tgatcgctgc ggcgatccgc gagctcttcg aggaggcggg cgtgcttctc gcgggcccgg
     7981 ccggcggcgg tgacggccgg cccgcggccg agtggctcgc gcaggagcgc gggcgcatcc
     8041 tggccagaga ggcgaccatc gcctcggtgc tgcgggacgg cgatctggtc gcccggaccg
     8101 acctgctcgg ctttcacgcc cactggatca cccccgaggt cgagccgcgc cgctacgaca
     8161 cgttcttctt caccgcgctc tgcccgccgg gacagcacgc cgacgaccag acccccgagg
     8221 ccgagagcgc agagtggatc gacccggcag ccatgctccg cgacgaacgc cccgccctca
     8281 tgccgccgac gatcgtctgc ctggaagacg tggccgccgc caccagtgcc gccggcctgg
     8341 tccgcatgcg ccgcgacgtc caggtcatcc aacccgtccc ggtgcggcac ggggacgggt
     8401 gggcgatgcg gatgcaggtg cggccatgac gactcccgac ccgcgcgggg tgctgccggc
     8461 ctggtcgggc ggcgagatca cgacgcacgc cacctgtgtg ctcgcgccca atccgggtcc
     8521 gatgaccctc gacggcacga acacctgggt cgtcatggca cccggcgccg gcgaggcgat
     8581 ggtcatcgac cccgggcccc tcgacgagga ccacctgcag gcggtatgcc gcgtggtcga
     8641 gcgccgcggc gcccgcgtca cggagacgat cctcacccac gcccacctcg accacagcga
     8701 gggcgcagcc cggttcgccg agctgaccgg ctgccgcgtg cgccccgtcg gccccggaca
     8761 cgacgacctg gccgacgggt ccgtggtgag cgcgggtggg gtggcgctgc gggtggtggc
     8821 gacacccggt cataccgccg actccctctc cttcgtcctc gaggcggacc acgccctcct
     8881 caccggcgac accatcctcg gccgcgggac gaccgtggtg gcgcaccccg acggcgaact
     8941 cgcggcatac ctcgactcgc tggagcgcat cagggcgctc actggcggcg gcgaggtggt
     9001 gacgatcctg cccggtcacg gccccgtcat acccgacgcc cacggcctgg tcggctacta
     9061 ccgcacccac cgccgcgagc ggctcgacca ggtgcgccag gcgctcgccg acggcgccac
     9121 cgatgtcgag ggcgtgctgc ggcgggtcta tgccgacgtg cccgaagcgg tctggcccgc
     9181 agcccggttg agcatccagg cccaactcga ctacctcgcc gcccgatagc gcaggcgcgg
     9241 ggtccccgcg agatatcgca gcgacgtcct agacgcccgc gcgcttgtgc aggcgctcga
     9301 cgttcatcag cagcaccgcg cgggcctcca ggcggatcca gccgcgcgtg gcgaagtcgg
     9361 ccagcgcctt gttgacggtc tcgcgggagg cgccgacgag ctgggcgagt tcctcctggg
     9421 tcaggtcgtg ggcgacgagc acgccctcct cgaccggacg gccgaaacgc tgggagagct
     9481 cgagtagggc cttggcgacg cggccgggca cgtcggtgaa gacgagatcg gcgatggatt
     9541 cgttggtgcg gcgcagccgg cgggccagcg ccgcgagcag ggtgaaggag acctgcgggc
     9601 gggcgctgag ataggcgtgc aaggcgtcgg tgccgagccc gacgagctgg gtctcggcga
     9661 cggcggtggc ggtggcggtg cgggggccgg gatcgaacag cgacagctcg ccgaacatct
     9721 cgccggggcc gaggatggcc agcagattct cccgcccgtc gtatgacgtg cggcccagct
     9781 tgaccttgcc ctcggcaatc acgtagagcc ggtcgccctg gtcgccctcg ttgaagagga
     9841 tgtcgccgcg ctccatgcgg atcgggatca tcgacgacag cagatcactg gttgcctgct
     9901 catcgagtcc ggcgaacagg ggcgagcgtc gtagcacgtc aagatccacg gtgcagagtt
     9961 tgccatgcac gcggtctccg cgtctcacct aggcccgcca acgcgccgac ggagggtcag
    10021 tcgctctggt ccaggcccac ggcgagcacc cggcaggcgt cgccgcgaac accgctcagg
    10081 gccacctcga tcccgtcggg gtggctgccg aagtcggcga tgaccaccga gccagcctcc
    10141 cgcatcagcc cgagccgggt cacctccccc tgcaccagcg agcgggccag gatcacggtg
    10201 ccgtcggcga agagcagccg ggccaccttg gggacggggg agtggtggat ggcccggagg
    10261 ggcacccggt ggctgcgcag gctggcgagc cggtcgctca ggaggcgccg ctgcgtgctg
    10321 ccggcgtcgg cgacgatccg ggccagccgg tcggccacct cgccgctgtc cgcgccgaac
    10381 ggatccgccg cgccccgccc gcggctcccg ccgcgacgga cgacgacggc gccgcgcaga
    10441 gcgagcacgg cgagggcgag ccactccata ccctcacctc ctcacgcgtc gccggcccgc
    10501 cggccgcggc gggcgcgtcg tcggtcccgg tctctaaggt atgaggtgtg cccgagtcct
    10561 cactagcccg cacccgccgc gcgcggcgga tgtatcgcga actcgcggac ttctatccct
    10621 acgcccactg cgagctggac ttcgagagcc cgctgcagtt gctcgtcgcg acgatcctgt
    10681 ccgcccagac caccgacgtc ggcgtcaaca aggtcacgcc ggtcctcttc gcccgctatc
    10741 ccgacgccgc ggcgctcgcc ggggccgacc gcaccgagat ggaggagatc gtcaagccga
    10801 ccgggttctt ccgggccaag acggacgcgt tgatcaagct cggggcggca ctggaggagc
    10861 ggtatggcgg cgtggtcccg ggccgcctga aggacctcgt cacgctgccc ggagtcgggc
    10921 gcaagacggc gaatgtggtg ttgggcaatg ctttcggtgt gccgggcatc acggtcgaca
    10981 cccacttcgg ccggctggtg cgccgcttcg ggtggaccga ggaggaggac ccggtcaagg
    11041 tcgaaacggc ggtcggcgcg ctcatccccc gcaaggactg gacgatgctc tcgcacgtgc
    11101 tgatcttcca cggccggcgc atctgccacg ccaaacgccc cgcctgcggc gcctgcccgg
    11161 tcgccaagct ctgcccgtcc ttcggcatcg gcgagaccga ccctggcaag gcgctgacgc
    11221 tcctgaagta cgaactggct cccggtcgcg ttgccgcaga ggcgaaccgg tgagcgaggc
    11281 gccggcttgg ctgcgcggga taccgcagcg cgtggcggag ctggacccgg cctacttccg
    11341 cgacttcacc ccaccgcccg acgcgacccg gcgctcggcg gtgctgatgc tcttcggccc
    11401 cgacccggac ggcggcgagg acgtcctgct cacccagcgc ggcacgaacc tgcgttcgca
    11461 cgccggccag gtgtccttcc ccggaggccg ggtcgacccg caggatgcgg gagccgtggg
    11521 cgccgccttg cgtgaagcgg aggaggaggt cggcctcgac cgcgccggcg tcgaggtcat
    11581 cgacacgctg ccgccgctct atctgtcgcc gagcgcgaat gtcgtgacgc ccatcctcgg
    11641 ctggtggcac agcccgtccg aggtgtgggc ggccagcgag atcgaggtcg aacgggtggt
    11701 gcgggtcaag gtcgaggacc tggtcaaccc ggcgaaccgc ttcctcgtgg acacgcccgt
    11761 cggctatgtc agtcccggct gggaggtgca cgggctgttc gtgtggggct tcacggccaa
    11821 gctgctcgac gtcaccttgc agctggcggg cctgatggag ccgtgggacg aggccgtccg
    11881 gcgcccgctg ccgccgcacc tgtggcggcg ctgaccgctg tcggctgtgc tcagacggag
    11941 gacttgaccg cctcgacggt ttccttggcc tcggtgatcg cctgctccgg caccggcttg
    12001 gcctggctga tggccttctt gcccatcagg ccgaggacgg cagctacgag gaagagcagg
    12061 gcggtgacga tgccgtagga ggcggagatc ggcagccaga tgtcgagcac ccgggccagg
    12121 gtgtggaaga ggaagatcag gccgagcagc ccgaggaagg cggcggcacc gagcagaccc
    12181 gcgcccttgc cgccgctctt caggccgccg gtcacctcgg ccttggcgag cgcgatctcc
    12241 gaacggacga gggtcgacat ctcccggctg gcgtcggcca ccagctgccc gatcgtgcgt
    12301 gacccgtccg tgtcgagcaa ccggcctgcc tcgtcgacgg gccggttctc aacagcgtca
    12361 taccgtgtgc ttcccacgct gccacgatag cgggtggatc gggcgttccg ggcgctcaac
    12421 ccagggtcgg ctgggctgca cccgccgcgg tcacctggtc tgctcgtact cgtagacgtc
    12481 gggtatgccg tcgccgtcgt cgtcctgctc ctcccgcgcc tcggccgcgc ggtaacgcgc
    12541 attgcggggc acgaggacca gggcggccag agcggctgcg gcgagcgagc cgagcaggac
    12601 cgcgagcttg acgtgctcgt cgcggtcgct gccggcgccg aacgcgagtt cgccgatgag
    12661 gagggagacg gtgaatccga tgccggtgag gaaactcagc ccgacgacgt cggcccagcc
    12721 caggccggga tcgaggtcgg ccttggtgaa ccgcgccgtc agataggtgc cgccgaagac
    12781 gccgacgacc ttgccgatca ccagcccggc catcacgccg agcgcgaccg ggtcggagat
    12841 cgcctcgccg aacccgccgc cgaccacccg cacaccggcg gcgaagaagg cgaagaccgg
    12901 cacggcgagg gctgcggaca gcgggcgcag gcggtgctcg aagacatggg cgacatcggt
    12961 tttcgggccg tgcacactga cggcgggcat ccgccccttc cgcgggcgga ccgggaccac
    13021 gagcccgagc agcaccccgg cgacggtggc gtggaccccg gacgcgtgga ccagccccca
    13081 cgtgagcagg gccagcggga cgagcagcag cgggtaggtg atccggcgct ggaccaggaa
    13141 ggtgaacgcc gccagcggcg cgacggcgag cagcagcggc atacccgcga agtcgctcgt
    13201 gtagccgatg gcgatgatga cgatggcgat gagatcgtcg accacggcga gggtgagcag
    13261 gaaggcgcgc agcgcggcag gcaggtggga gccaaggacg gcgagcacgg cgagcgcgaa
    13321 cgcgatatcg gtcgcggacg ggatcgccca gccgcgcagc gcatcgccgg tgccaccggt
    13381 cgcgcgcgtg acgatcaggt aggtcgccgc ggggacgatc accccgcaca cggcggcgac
    13441 gatcggcacg gcggcgcgcc tgggatcacg caggtcgccg acgaggaact cgcgtttcag
    13501 ttccagcccc gcgacgaaga agaagatcgc gagcaggccg tccgccgccc agtgaccgag
    13561 cgtgagcttc aggtgccccc agtccacgcc gaccgacagg tcgcgcaggg cggcgtagga
    13621 ctcgtgccac gggctgttgg cccaggcgat ggcgaggagg gcggcgagca gcagcagggc
    13681 gccgcccacg gtctcggtgc gcagcgccgc tttgagcgcg gtggcctcgg gccaggacgg
    13741 acgcgagaaa atgcggccgc ggacaggagg gatcgtcatg acggtcacct tcgggtcggg
    13801 tcgttcgggg taaatgccga ccagacttcc cggcgctccg tcacctattt tacccgcttc
    13861 gggtatgccg tccgcccacc tcagcgggcg gcatccggtg ggtcgtcggg tggggcgggc
    13921 gcggcccaac gcgatccggc ggtcgaggtg ctcggcggtt tgcgattcca ggtcgcagtc
    13981 ggctccgggg tgcccccgct ggcggctgtc ggaggccgtg tctacagtgc cggacatgac
    14041 gacgggggat gagcgcacgg cgctcttgga tcagttgaag cagctgtggg agctcgtggg
    14101 tggccacgag gtgcgggccg gtgggcctgc attggtcgag gagctgaccg ggcacttcgg
    14161 cagtgcgccg cacgagatgg cggtgctcac cgaagaggtg gcgaaccacc gcttcgtcga
    14221 cttcgacatc gccctcgagg ttgttgcggc gcgcgacgcc ggacatcggg cgatcggcat
    14281 cggcggcggc gagtctcgct accaccacaa cttctccgac atgatccagc cgccgggcgt
    14341 cggcttcggc ggggggcgct tcccgctcgc ccaggtcgac tacgccaatg tcgccaccgg
    14401 ccccgacacc gagcgtgccg cgattgcgtt cgggatccgc ctcttccggt atgccggcgt
    14461 gccggtcgcc gtgctgctgc gcaaggcgaa tccctatcgc ggacgcggtt ccgcgacgct
    14521 cgaggtcctc tgtcccgacc agccgacggc cgcccgcctg ctcgacgagc tgcgcgagat
    14581 ctcgttgcag cgcagcgtgc ttcgcggtca ggtcgtcggc ctcgccaact cgggatacga
    14641 gcaggccgaa gaggggctga cgttcattgc gcgccccaac ctgacggccg ctcaggtgat
    14701 cctccccgag ggcgtcctgg agcgggtggt cgatcacgtg gtcggcatcg ccgcccacgc
    14761 cgagatgctc gcggcgcacg gccagcacct caagcgcggg gtgctgctct tcggcccgcc
    14821 cggctccggc aagacgcaca ccgtgcggca cctgctcagc atcacgcccg gacacacggt
    14881 cgtgctcctc gcgggggaca cgctgcggtt cgtcggcctc gccgccaagc tcgcccgcgc
    14941 catgcagccg gcgatcgtcg tgctcgagga ctgcgacctg atcgccgagg accgcgacat
    15001 gcaccacggc gccaagcccc tgttgttcga ggtgctcgac gcgatggacg gcctcgacgc
    15061 cgatgccgat gtcaccttcc tgctgacgac caaccgggtc gaggcgatgg agcgggcgct
    15121 cgcgcagcgt cccggccggg tcgacctggc cgtcgaggtg ccgctgcccg acgaggccgg
    15181 ccggctcgcg ctgctccggc tctatgcccc ggtcggcgcc ttcggtgagg aggtgctcgc
    15241 cgagacggcg gcccgcaccg cgggcacgac cgcgtcattc agcaaggaac tgatccgccg
    15301 ggccgtgctc gccgccgcca aggagggact ggagccctcg gatgcgcacc tgcgcggcgc
    15361 gaacgatgtc atcctcgccg acggcgagcg tctctcccgg gcgctgctcg gtgtccagga
    15421 ttccggggtg cccggcaacg gcgccgatcc gcgcgccact cccgacgggt cgggcagctt
    15481 tcacctgatc tgaccggccg tcaggcgtgg gcgtgctcgc cgaggagccg gtgcgccgag
    15541 acgtcgtcga cggtgtcgaa gaagccgccg acgacgtcct cgagttggtc gaggttctcg
    15601 gcggcgaaga gcaccgactg gtaggtcgtg atgtcgtagg ggatcgtggc catctggtgc
    15661 aggtcgagcg gacggatctc catgtgggag taggcgtcca tctccccgaa cgaggacagg
    15721 ataccggcgc cgtaggcctt gaggatgccc tcctcccaga tcacgccgaa ctccatcgag
    15781 aaccagaaga cgtcggcgac ggccttgact gcggcgtcgg tctccagccg gcgggtggca
    15841 gccccggcgg cctcggtcag ccgggcgaaa cgcggcgacg cgagctggtt ggcgtggccg
    15901 atgacctcgt ggatgacgtc gggttcgggg gtgtagagcg gctggctggg gtggcgcagg
    15961 tactgcgtgg agtggaacac gcgctcaccc agcgccccgt agaactcgcg cagcggcacc
    16021 aggccggggg ccgcggcata cgaccatccg gtgagcggga agaggtgctc ggtcaccatc
    16081 gccagctgcg gcacccgatc ggcgggcagc ccgagggcgg ccttgccgcg caggtattcg
    16141 gagtgggcgt ggcgttggtg cttgggcgcg agttcggcgc cggcgatccg ccagacctcg
    16201 ttctcctcgg ccgtgtagcc gatcagcggc acgggggtgc cgggctccca ggcgagggcg
    16261 gcggccgcga tctcgccgcg gcgggccagg tatgccgggt cgttcagtcc cggatgatcg
    16321 gcgcccaagt gcacctcgac ggagccgtcg gcgccgacgg tcacggggga gtagagctgg
    16381 ccttcctcga acatggcggc cccttttcgt gcgtgcgcga ccacctcgtc gcgcggttgg
    16441 cccagggaac cagatcggga gcgagctggc tagagtgtct tccgagggcg ggcagttagc
    16501 gcactattgc ctagcaatca gcgggccatg ctggacgaaa tgtccagtat ggcccgctga
    16561 cgggtcggcg gtcgggtcag cctccggagc cgagcccctg ggcgatggag ttcatcaccg
    16621 aggagtcggc gagggtggtg acatcgccga tctcgcggcc ctcggcgacg tccttgagca
    16681 accggcgcat gatcttgcca gagcgggtct tgggcagttc gggcacgatc atgatcgtgc
    16741 gcggcttggc gatcgggccg atctccttgg ccacgtggtc gcgcaactcc ttgaccagat
    16801 ccccgccctc cccgacctcg tcggcctcct cgatgtgctc ctcgcgcagg atgacgaagg
    16861 ccacgaccgc ctggccggtc gtctcgtcgt tggcaccgac gacggccgcc tcggcgacct
    16921 tcgggtggct gacgagcgcc gactcgatct cggcggtgga caggcggtgc ccggagacgt
    16981 tcatgacgtc gtcgacccgg cccagcagcc agatgttgcc cttctcgtca tatttcgccc
    17041 cgtcgccggc gaagtagtac ttcgggccga accggctcca gtaggtctgc ttgtaacgct
    17101 ccgggtcgcc ccagatgccg cgcagcatcg ccggccacgg cttggtgagc accaggtagc
    17161 cgaccttctc ggcctcggtg aacggccggc cctcgtcgtc gaggatctcg gcgctgatgc
    17221 cggggatggg cttctgcgcc gacccgggct cgagcgtggt gacgcccggc agcggcgaga
    17281 tcatgatcgc cccggtctcg gtctgccacc acgtgtcgac gatcgggcac cggtcgccgc
    17341 cgatccactt gcgataccag agccaggcct cggggttgat cggctcgccg acggagccga
    17401 gcaggcgcag cgaggacatg tcgaacttct cggggatctc gtggccccac ttcatgaacg
    17461 tgcgcaccgc ggttggggcg gtgtagagga tcgtcacctt gtacttctcg acgatctccc
    17521 accagcggcc ctggtgcggg gtgtcggggg tgccctcgta catcacctgc gtcgccccat
    17581 tggccagcgg tccgtagacg atgtaggagt ggccggtcac ccagccgatg tcggcggtgc
    17641 accaatagac atcggtgtcg cggtgcacgt cgtgcacgac ggcgttggtg taggcgcact
    17701 gggtcaggta gccgccggtc gtgtggaaga tgcccttcgg cttcccggtc gtgccggagg
    17761 tgtagaggat gaacagcggg tgctcgctgt cgtggggctg ggcctcgtgg gtctcgctcg
    17821 cctcgtccac cacgtccgac caccagatgt cacggccctc ggtccagggg gtgtcctggc
    17881 cggtgcgctt gacgacgagg accttctcga cggtcgtctc ggcgtgctcg agtgcggcgt
    17941 cgacggccgg cttcagagcg ctcggggcgc cgcggcgata gccgccgtcg gcggtgatga
    18001 ccagcttgca cgccgcgtcg ttgatgcggg tgttgagcgc ctccgcggag aagccgccga
    18061 agacgaccga gtgcggtgcg ccgatccgcg cgcacgcgag catcgcgatg gccgcctcgg
    18121 ggatcatcgg cagatagatg gcgacggtgt cgcccttgcc gagtccgaga gcctcgagcg
    18181 cgttagcggc cttgcagacc tcccgttgca gatcggcata cgtgatggtg cgggtgtcgc
    18241 ccggttcgcc ctcgaagtgg atggcgacct tgtcgccgcg gccctcctcg acatgccggt
    18301 ccacgcagtt gtatgccgcg ttgagctcgc cgccggcata ccacttggcg aacggggcgt
    18361 ccgaccagtc gagggtctgc tcgaaatcct tcgcccacgt gatccgggtg cgggcctgct
    18421 cggcccagaa gccctcatgg tcggcggccg cggcgtcata catctccgct gtgccattgg
    18481 cctgcgccgc gaacgcggcg tccggcggga acgtggggtg gctggccgac aggctctctt
    18541 ccgtggacac gggtggctcc ttcgcgaaac gacgatgttg gcacccaccc tggcaggcgg
    18601 acgccaacac ctcaaccgaa ccttcgcggc ggcgcgttgg gcgggcacct ggctcgccca
    18661 gcggtcgggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agacgccaac
    18781 atcctgatca ccggggagag cgggtcgggc aagtcgcacc tggcccgtca tggatccccg
    18841 ccgcacccgg cacgtccggc actggcacaa gtacacgacc caagcgctgc cggaggatct
    18901 gtggttcacc ttcaccgagc cgtccggcaa ggtcatcggc cacgcctcga ccatcgccga
    18961 gttcgtgcgt ggcgtccggt ccgtggacgc gggtgtgatc tggtggcatc tttcgcgcgg
    19021 cgacttctcc cggtggctcg cgatcagcct cggtgatccg gaccccgccg ccctggccgc
    19081 cactaccgag cgcgacgcga tcgcccgcca cgccgccgac attcactccg cccggcgccg
    19141 gctgctcgcg ctgatcgagg acttgtaccg actcgatccc gctcccgagg ccgacgccga
    19201 gcccgagccg gcctgaccga cccggcccgc actccgcata cccggaaaaa agagcgaagc
    19261 gggcggcata ccgacgatcg gtatgtcgcc cgcttgcgtt gcgggagaat cgggctcagt
    19321 gctccagcgc cttcccgacg ccgtggccgg tgagggagcg cacctccatc tccgcatact
    19381 tttccgcgtc gaactccttg gacgtgaccg agccgagcca gccgaggaag aaggccagcg
    19441 ggatcgagac cagacccggg ttgtcgagcg ggaaccagtg gaagtcgatg tccgggttgg
    19501 tgaccatcga cggggacagg ccggtcttgg ggtccgccgc cttgcccgag acgaccgggc
    19561 tgaagatgat cagcccgatg gaggcgatca ggccgccgta gatcgaccag agtgcgccgc
    19621 gggtgttgaa gcgcttccag aacagcgagt agaggatcgt cggcagattc gccgacgccg
    19681 cgacggcgaa cgcgagcgcc acgaggaagg cgatgttctg gctcttggcg agcatcccac
    19741 cgaagatggc gaccaggccg atggcgatcg ccgagatgcg cgcgatgcgc acctcctgct
    19801 cctgcgtcga ctggccccgc ttgaagacct ccttgtagag gtcgtgcgcg aacgtcgccg
    19861 acgcggtgat cgtcaggccg gcgacgaccg cgaggatggt cgcgaaggcg atgccggaga
    19921 cgatgccgag catccacgag ccgccgagtt cgaaggcgag cagcggtgcc gcggagttct
    19981 gcttgccggg ggccgcgttg atcgcgtccg ggccgacaag ggcgccggcg ccgaagccga
    20041 tgaccagggt cagcagatag aagccgccga tgagccagat ggcccactcg accgacttgc
    20101 gggcggcctt gctcgtcggg acggtgtaga agcgctgcag cacgtgcggc agacccgcgg
    20161 tgccgagaac caaggccatc gacaaggaga tgaagtcgat cttcgccaac gtggtcacgc
    20221 cgtacttggt gccgtagtgc agcaggttct ccgcgcccgc ggcggtcttc gggttggtcg
    20281 aggtcttcgc gacatcgacg gagtcggtga agaggccgga caggctgaag ccgtacttgc
    20341 ccagcaccca gacggtcatg atcgccgcgg ccgagatcag cagcacagcc ttgatgatct
    20401 gcacccacgt ggtgcccttc atgcccccga tgagcacgta ggtgaccatg accgcgccga
    20461 cgacggcgat gacgaggttc tgcgccgtct caccggacac gccgagcagg agcgagacca
    20521 gcccgcccgc gccggccatc tgagccagca ggtagaagag cgagacacac agcaccgaca
    20581 ccgaggtcgc gatgcggacc ggacgctgct tgaggcggta ggagaggacg tcggccatcg
    20641 tgaaccggcc ggtgttgcgc atcagctcgg cgacgagcag gagcgcgacg agccaggcga
    20701 cgaggaagcc gatggagtag aggaagccgt ccatgccctg cagggcgatg gcgccggcga
    20761 tgccgaggaa ggaggccgcc gacagatagt cgccggcgat cgcgatgccg ttctgccggc
    20821 cggagaaggc gccgccggcc gtgtagtact gctcggcggt cttcttgccg gaggccacct
    20881 tcacgacgac cgtcaaggtg acgagcacga agatgacgaa aatggcgata ttgagcttgg
    20941 cgttgccgac tgtcgtggcg gcggcgagag aggtcagtgc ggccatgggt cagtgctcct
    21001 tgacgccgtc gagtcgggag ccgatcgcgt cggccagggg gtcgagttcg cggttggccc
    21061 accgtgcgta gatgaacgtg atcgcgaagg tcgtgaggaa ctgcaggaac ccgaagacca
    21121 ggccgatcgt gatcttgccg aacaagaatc ggttcatgaa gtcctgggcg aagatggaca
    21181 agaggacgta gaggaagtac cagacaagga aaaaggccgt caccgggaag acgaagccgc
    21241 ggaacttctt gcgcagcgtc tggaactctt cactggactg gaccgcctga taggcctcgc
    21301 tggtggtggg ctgttgggcg ctcattgcgg ccacctccat gctgacgcgg cgtgcctcgg
    21361 tgcgcgccgt ctgtggggca cagctttctg ggtatgccgc ggccccggct agcaggcacg
    21421 cgcggtgtcg cgccgggcgg gtgccggggt cgccggatgg gtccgcgtca cgcgccgagc
    21481 ggttggtttc gcgcgatgag cggccggatc acggggtgat cacggtggca ggagcgattc
    21541 gggggtatgc agtcggctga gggtccggtt ggtgtgcggg gggactttgc cgggggtgag
    21601 ccgggagacg acgaccatcg tcacgaacgc gagcggcacg gaccaggccg ccggttgggc
    21661 gagcagcagc ccgggccagc cgtgcggttc gacgtggagg aaggccgcgc ccagggtgac
    21721 cagggcactg agggtgccga ccgccaggcc cgccagggcg ccggtggtgg tgaggcggtg
    21781 ccaccacacg ccgagcacga ggaggggggc gaaggtcgag gccgccacgg cgaaggcgag
    21841 ggtgacggtc gcggcgaggc cgagcggctc gacgacgcgg gagagcaggg cggggacgat
    21901 gacggcgacg gtggccgcca tgcggtagct caccgaattg tgcgcgtcct ccccggtgcg
    21961 ccgcgccacg agcggccgca ggacgtcctg atcgacgacg ccggcgaccg agatgaccag
    22021 accggacgcg gtgctgagga aggcggcgaa ggcgccgccg gcgagcaccg cggtcagcag
    22081 cgtcgcgctg cggccgtcgg tcatcgcctc cggcagctgc agcgccatcg tgtccacgcg
    22141 ggtgccgggg gcgaggtgcg gcagatggat gtggccgagc acggcataca ccggagggaa
    22201 gatgtagaac ccgcccagca gcgcgaggac accgacggtc gtgcggcggg cgtcgcggcc
    22261 gtcggggttg gtgtagaagc ggacgagcac gtggggcagc cccatcgtcc cgaggcacag
    22321 tgcaagcatc gtgctgtatg tcgtgtagag gcccgccggg tcggtgctcg gcaccgccca
    22381 gtcgccgagt tccaccggcc cggaggagtg ttggctccag atcgccagca gcacgaaggc
    22441 ggggatcgcg atggcggtca gcttgatcca gtactgcatc gcctgcacca gcgtgatcga
    22501 gcgcatgccg ccgaccgcga ccgcggcgag cacgacgacg gagacgagca acgcgccgat
    22561 ccacgcgggc cagccggtgg cctgccgcag cgccaggccc gccccctgga actggggaag
    22621 cagataaagg aacccgatcc cgaccgtgag cagggaggcg aagccgcgga tctgccggga
    22681 ctccaggcgc aactcggcga agtcggagag ggtgtaggcg ccggagcggc gcagcggggc
    22741 ggcgacgagg acgagcagca ccaggtagcc gacggtccac ccgacgggga accagaggat
    22801 gtcgatcccc cgctgataca tcagtcccgc aaccccgaga aaggatgcgg cagaaagata
    22861 ttcgcccgcg atggcgctcg cgttgcggcg cggcgtgacc gtgcgtccgg cgacgtagaa
    22921 gtcggaggtg gcccgcgaca tacgcagccc caaggtgccg accgtgacgg tgaggacgca
    22981 gacgagcgcg atggccgcca ggctcgccgg acgcatcagc cgcgcccgac cacgtcgacg
    23041 aaggccgcct cgagacgctc gctcgcgcgc acgtagtagc gggcgctgat gacgatcgcg
    23101 ggatagacga ccagaccgag ggtgacccac ggcaggggga gcgggccgag ggtgacggtg
    23161 cgcgtctgcg gcagcatcgc aaagaggacc gggacgccgc ccaggagcag gaccgcgagc
    23221 agcagcagac tcagcgcgag ccgcagttgg gcgcgcatca acccgcggac gtagatctcg
    23281 ccgaggacgg tctggtcgct cagttcgtcc gcaacgggcc gggcggtatg ccgtggggcc
    23341 gagcgccgcg acgccgtcac gcgaacgcgc tccgacggcg gccgctgcgc gggactcatg
    23401 gcgccgacgg gcgggcgagc agttgctggc gcaggtcgcg catgtgccgc cggctcacct
    23461 gcaggtcggc ctcgcccacg cggaccgtcg cgcggccgcg gtccatccgc acctgggtca
    23521 catgcttgag cgacaccagg gtgctgcggt ggatccgaac gaatccggct gccgcccaac
    23581 gctcttcgag ggtgttgagg gagatgcgca cgaggtggga gccgatctcg gtatgcagcc
    23641 gggcgtagtc gccgtgagcc tggacgtagc ggatgtcctt gcgggagacg aaggtcgtca
    23701 ccccgccgag ttcgacggga atggtctcat cgtcgtcctg gggttcggcc ggtgcgctga
    23761 tggcggcggc cgcgacccgg cggaccgcct cggccagccg ctccgggcgc accggcttca
    23821 tgacgtagtc gatggcgtcg atcgcgaagg cgtcgacggc gtgctggtcg tgggcggtga
    23881 cgaagacgac cgccggacgg gtggcgaagc gcgcgaggac gcgagccaac tccataccgt
    23941 caagtcccgg catggagatg tcggagaaga cgacatcgac cttttcgtcc tcgaggacgc
    24001 ggagggcatc ggtgccggac gcggcgacga ggacatgcgc aatgcgctcg tcctgctcga
    24061 gcaggtatcg caactcggat cgcgcggggg cctcgtcgtc gacgacgaga gcaacgaggg
    24121 caggactgct gatcatgatc cggccagcct attgtcgccg gccgtgatcg cggcagggag
    24181 gaacttgggg acgcggaaac tgacccgcat gcccgccccg ggagcggtct cgacgaccag
    24241 gccgcgttcg tcgccgtaga tcgtgcgcag gcgtgcatcg acattgccca gtccgtgcga
    24301 gtcggtgtcg gcggtcccgt cgaggacggc ctggatgtgg gccgggtcgg ctccgacacc
    24361 gtcgtcctcg acgacgatct cggcctccgc gccgcggtcg gccgcggtga tcgaaaccgt
    24421 atgggcgcct tcgagattgg cgatgccatg acgcaccgca ttctcgacga gcggctggat
    24481 ggcgaggtag ggcacggtca ccgacagcac ctccggcgcg atgagcagcg agacctgcag
    24541 gcgctcaccg aaacgcgcct gctccaggac caggtagcgc tccacattgc gcagctcgtc
    24601 ggcaagcgtc gtgaacgcgc cccccttgcg gagggcgtag cgggtgaagt cggcgaactc
    24661 gagcagcagg tcgcgggcac gggccgggtc ggtgcgcacg aaggaggcga tcgcaccgag
    24721 cgagttgtag atgaagtgcg gtgagatctg cgcgcgcagg gcggccagct cggcctcggt
    24781 ggcgcgagaa cgttccttgc tcagctcggc cagttcgacc tgcgccgaca gccagccggc
    24841 gacctcggcc gtcgcgcggg ccaggccggc gttggcatgc gggccgtatg ccgccagcgc
    24901 gccgaccacc cgcccctcgc agacgatcgg ggcgatgacg gcagcccgga tggcgcagtc
    24961 cacatcggtg caggcgacct cgccgtggtc gaggacggcg gtatggccgt cccgcagcac
    25021 cggttcggcg agcttggcga cggcgccggc gtggtggttg tgggcgccct cccaggtgac
    25081 ggttccgctc tggtcgatca gggaaagggc cggggtgtcg aggagggtcc gcaggtggcg
    25141 tgcggccttg gccccgccgg ccggcgtcag ccctgatccg aggtggcgtg ccgcccgggc
    25201 cgccgtatgg agcgtctcgt aggtcgcccg gttggcgtcg ctgaggaatc cccgggaccg
    25261 gccgcgcacc gtgaccacca ggcggatccc cagccagatg aggaagacga tcagcacggt
    25321 caccatcgcc gtgggcagat cgaaacctcg catcccgcca gggtagggct cacctcgcag
    25381 gtcgcgcagg aagactcgga tgccgccgcc gggcggcccc ggaggggaac ggtgcgnnnn
    25441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccc cggctggggg ggaacaggtg
    25561 cggggtgcgg gtgccgccgg gcggccccgg cggggaacgg tgcggccccg gctggggggg
    25621 aacaggtgcg gggtgcgggt gccgccgggc ggccccggcc gggaacggtg cggggaacgg
    25681 tgcggccccg gctggggggg aacagccggg gccgctgcgg gcgatgccct cggggggtag
    25741 gcaccacacg caggttgctc aacccgaagg gagcgacctg cctcaatcct tgggcaaatt
    25801 ggcggatcat gcaacagcaa cgccgcatgt ggcgccgaca acttttcgtg ggacgagata
    25861 aatgcggcgt acgcccgccc accgcatagg gtgccggggt gtccgatgcg ctcgccgctc
    25921 tccagtccct cgccgctgac gagcgggtca ccgcggcgtc ggaccgggcg cgggaggcgt
    25981 gcacgcagtt gcgctggcac caggcgctgc gccgccgcat accggaggcg gcagccgaat
    26041 cgcgcgtgcg gggagcgtgg gccagcggcg aactggacgg cgcgcgcagc agcatcgaga
    26101 tcgtccggga cctgatgcgc ggggcccgcg aaccgtcgcc cgacccggac cccgcagagc
    26161 gggtgctgca cggggcgatc gcggcgacgg cggcgacgga gggtctggtg gcgtcggtgc
    26221 gttcggctcc gcgacaggca tacgcccggc tccacctcgc cgctgcctct cacctggtgg
    26281 acagcggcga cctcggccgg ccgcggacga gcgccgagtg cgcggagttc tccctcctgg
    26341 ggccggcccc ggatgccgcc gaagcggccg cccggctgtt cgcgctcgat gacgtcatcg
    26401 cagccgacga cctgcccacc ctgctcgtgg tcgccatcgc gcacgctgag atcgtccatg
    26461 cccgcccgtt cgcccgcggc aacggtcttg tcgcccgggc gctgaatcgc gttctgctgc
    26521 aggcgtgcgg actcgacccc acgggggtgg tggtcccgga agccggccac ggcgcccagg
    26581 ggggtgtcgc ctatcacgga gccttacagg catacggttc gggctccctc gcgggcctga
    26641 tgctgtggat cgaccactgc gccgacgccg tcgtggcggg tgcgcgggag ggcggcgaga
    26701 tcgccgatgc cgttctcgcg ggccggtttt cggcgacacc cacgctgccg ggctgacgaa
    26761 gttatccaca gccttgctgg gtgtcggtag cccgatcggc cggttgtcgg cacgctgagg
    26821 ggcatgacga ttcttggggt ggtgagtggg tccggcggcg ccggctgctc gacattggcg
    26881 gccgcgctgg gggtgcaggc ggcggcgctc gggggcacgg ccgtcgtcgt cgacggcgac
    26941 ccggtctgcg gcggcctgca ggtcacgaca ggggtggagc acctgcccgg ccaccgctgg
    27001 gccgaactcg ccgaggtcga cggggtggtc gatggctcgc ggctgcgcga acggcttccg
    27061 caggcggacg gctgcgcggt gttgtcgggt ggccgggtca gttccaccct gactgcgctg
    27121 agcccggaac cggtgccgcc caccgctttc gacgccgttc tcgacggcct ggagcgctcc
    27181 tgcgatctcg tcgtcgtcga ttggggccgg cagctgcggc cggggcaggg cgcgagtctc
    27241 tatctgctcc cgctcaccgc tcgcggcatc gccgatgcgc aggcatggct tgcgcagttc
    27301 gacgccgagc agttggccgg cgtcgtcacc cggtcccgtc gtggccggcg caagctggcc
    27361 gaggccgctg ctgcaggtct cgggctgccc ctgctcggcg aggtggtcga cgatcggcgg
    27421 gtcgccgtgg ccgagcggcg gggtggtatg ccgggtgccg gccgcggacc gctcgcccgg
    27481 ctggcgcgca acatcgtcgg ggcggtggtg caggccgagc aggcacgcac cggtcgcgct
    27541 gatcgggtgc cgcgcggtcg gcggcgggtg gcgacgggcc gggcgtgatg cgtttcgacg
    27601 acgggatcga tgccgcggtc cgggccggcc gggtgcccga ccggcgcagc atccgcgctg
    27661 tcgtggacac ggccgccgaa cggctgggcg ctcgcggcgt cacccgcacc gaggccgtgc
    27721 tcgaagccga attggccggc ctggggccac tggccggcct ggcccgcgcg gcgggagtga
    27781 ccgacatcct ggtcaacggt gacgcgcaga cgtgggccga ccgcggcgcc ggcctggaac
    27841 gggtgcccga gcacgatttc gagtcgcccg aacacgtgcg ccgtttcgcc gttcgcctcg
    27901 ccggcgtggc gggccgacgg ctcgacgacg cgcagccgtg ggtggacggc ctgctgcccg
    27961 gcgccgtccg gttgcacgcg atcctgccgc cgctctcctc gggtggcacg tgcatcagcc
    28021 tgcggatgtt gcggcgcgac gccgtgcggc tgaccgacct ggagcgggtg gagatgtgca
    28081 ccccggacca gggtgcccgg ctgcgcgcca tgatcgccaa ccgcgacgcc ttcgtcgtca
    28141 cgggtggcac cggggcgggc aagacgaccc tgctcgcggc gttgctcggc gaagtcgacc
    28201 ccagtgaccg gatcctggtg gtcgaggacg tcctggagat tcccctcaac agcggtcacg
    28261 tcgtgcgcct gcaggcgcgc ccgcccaatg tcgagggcca gggatcggtc acgctcgtcg
    28321 atctggtgcg tcaggcgctg cgtatgcgcc ccgaccgggt cgtcgtcggc gaggtccggg
    28381 gcgccgaggt ccgagaactg ttgcaggcgt tgaacaccgg ccacgaaggc gggtgcgcga
    28441 cgatgcatgc caacaaaccg gccgacgttc cggcccggct cgaagcgctg ggggcgctcg
    28501 ccgggatgtc gcccgccgcc gttcgcgcac aggtcgctac ggccctgaaa accgttgtgc
    28561 acgtggcccg tacgcccgtc ggccgccgga tcaccgccat cgaggatctg tgagccgtgg
    28621 gccggatcct cgctgccttg ttgctgctgc tcggcgggta tgccgtgtgg ccccgccgcc
    28681 gcgggatgcg ccggcgccgg gaggcggcga catccgacac acgcgtggat tcccttgcgg
    28741 cagtgcttga gtcggtcgcc ctgtgcctgc aggccggcct caccccggtg caggctgtgg
    28801 acgtcgccgt gcagcagcgc cgcgactccg ccgccgagca ggacgacgcg atcggctcgg
    28861 tgctggccga ggtgcagcgc gccctgcacc gtggcgcgcc gggaggcccc gcctgggccc
    28921 ggcacagcga ccagatcccc gaactgcggg tcgtggccgg ggcctggacc ctgaccgagg
    28981 ccagcggatc ctcactccac ccggcggtca tgtggtcggt cgcgcaactg cgtgagaagc
    29041 gcgcggcaaa ggagcggctc tcggccgtca ccgcgggcac gacctcgtcc atgatgctga
    29101 tgctggccct gcccctggcg ggagttccga tcggcctgct cgtcggcgtc cctccgggtg
    29161 aactctacgg ctccttcccg gccggggtcg cgctggtggt cgggctgtcc ctggcggcct
    29221 ccggagccgt gatgtcgaag cggctgctcc gtaaagccct gcagcccaag aagatcagtg
    29281 ccgaagaggg attgcccgac gcgacactcg acgacgtcgc cgatgccgcg ctgatgctgc
    29341 agctcgccct gagctccggc gccggaatcg tcgagagcat cgaacaggtc gccgtcgtct
    29401 cgccgcatcg gatccgcacc gagttgcggc gcgtcgtggc cggctatcgc tggggcctgg
    29461 ggcatcgcac cgcgtggtcg tatgccgatg tctcctggga gccggtctcc gccgccctcg
    29521 cgctgtcgct ggagcatggc gccgcgccgg ccgcgagtgt caaggccgcc gggcagcggc
    29581 tggccgagtc ggagaagtcg cgcctgcagg cggcggccgg tcgggccgag accttcctcc
    29641 tcatcccgct cgtggtcttc ttcctgcccg cgttcgcgct gaccacggtc atcccgctcg
    29701 tcattgctct cgtcccccgc ggtggcctgt tctgaacgag ccaacgccgc aggcgcagtc
    29761 gagtggtggc tgtcggcacg ctttcggccc ggaaccgtac cggcactcac cactcgaatc
    29821 cgacgctgct ggtcttgtcg agaaggtcgt tcctcggggc cgcggacagg gccggttttc
    29881 cacaagcgga acgccaactt tcgttgatcc acagggtttt tcgcgcctct ggcggcggcc
    29941 tttcgggcac agaaaagtag aggcatcgac cggcgcgggg ccggtcacct tccgaaagga
    30001 acagcacatg tcgcacaagc tcgctgcacg tttcaccgcc accaaggcca cgctgctgga
    30061 ccgggccacg gccaagaacg accaggaggg tctgaccacc atcgagtggg cgctggtcac
    30121 cctcatggtc tgcgccttcg ccgttaccgc cctgggcatc ctcagctcgt tcggcgtcgc
    30181 ggagatcggg aagctgatca agaacgcctt cggcggcaag gcctgacgag atgagtccgt
    30241 cgtgctgcag acccacggtg gcttgggcgc cggccgcggg gcgcggcggg caccgtcacc
    30301 ctggcgccgg cgcagcccgt gaccggctgg ttcgacgaga gtcgggtctg gtgacgatcg
    30361 aattggcgtt cgggatgatc gtgttcgcgc tcctcctgct gctcacgctg accgcgatcg
    30421 ccaccggcct cgaccacatc cgggtctcgg aggccagccg ggtcggagcc cggctggccg
    30481 cccggtccga gccggtctcg cacgtcatcg ccgccgccaa ggaccaggca ccggatgggt
    30541 cggtggtcag cgtcaccggc gacggcgggt cggtcgtcgt caccgtccgg gcgcccgagc
    30601 gagcgccctt cgcccgcctg ggctgggatg tggctgccgt cgcccgatcc gtcgcccccc
    30661 gggagctgcg gccatgaccg cccgatggtc gcccagcccc cggaggccgt cgccggcccc
    30721 cagcaggcac cacgaggcgg gttccggcac catcctgacg atcgtgctgg tggccgcgtt
    30781 gatcatcctg ctgccggcca cccttgggct ggtgacggcg gtgcaggcca agcagcgcgc
    30841 gcgggcagcg gccgacctcg gggcgatcgc tgcggtgtcg aacttcatgg ccgggctgga
    30901 tgccgccaca tcgtgccggc gtgctgccgg cttcgtcacg gccaatgcgg cccagcccac
    30961 cgggtgttac atcacgccgg agggcgcggc gaccgtcacg gcgcgggtga ccgcgcgcct
    31021 gccggtcgtc gggatgcgca cctccacgac ctcggctcgc gccggcccgg tccggcaacg
    31081 gtgacgcgcc tcggacaggc ctccggcccc gtccaccgaa gcggacgggg ccggaagggg
    31141 gtcgagaaga cgcgcgggcc cccgagacgg ccagccgggt cagcggacca gcagggtcag
    31201 gcggcagtcc ccctgggggg ccggcccggg tcagcggcgg cagtccccct ggggggccag
    31261 ccgggtcagc ggcagtgccc ctggggggcc agccggttcg gcggcagccc ccctgggggg
    31321 ccagccgggt cagcggcggc cgtctccggg ggtccagccg gcgtcattgc gattgaccga
    31381 gtcggtgcgg ttgacggggt cggtgatgct gcggccacgc tcgtcggcag cagcacgttc
    31441 ggcgcgcagg cgctcggcgg cggcggcttc cgaggcgcgc tggttctcgg cggcctcccg
    31501 ctcgagttcc ttgcgctcac gacgacggcg ggcaccggcg ctgatgccgg cccggatcag
    31561 atagaggccg attcccaggg cgcaggccag accgaagccg atgagcagca acgccatcgg
    31621 cgagaacccg atggtgacac cgagcgcggt gaagtcgacg gtgtgatcgc ccaccatgtc
    31681 catcagggcc agatagagcc acgtgccagc cagggccgcg agaaggatga aaagcaaacc
    31741 gagcacgatc attgggatcg agcactcctt cgtgtctgcc gacccaggag cccgggttcg
    31801 actggtgtcc cggcgtgctg ccggtcggtc caccggttgc aggaccgtgc caacaacggt
    31861 agccgggcgc gggcgattcc cggctcctgg cgtccggacg agtcacgtct cccgactcac
    31921 gtcacggacg cgggactccc tccgcccggg gaccaccacc cggctccggc gggaccccgc
    31981 tccgcggatc ccgcgcgctc agccgggacc tctcacccgg gcctgctcag cccggaccgc
    32041 gcgccaactc gccgagcacc aggtcgagca cggtgatcgc cccgggcttg tccaacggct
    32101 cgttgccgct gccgcacttc ggcgacacca cgcaccgggg gcagccgcgc tggcacgggc
    32161 actcggcgat caacgcgcgg gtcgcactca gccactgcgg caggatctcg aacccccggg
    32221 cggcatagcc cgcgccgccg gcatacccgt catagacgag gatcgtcggt tcgcccgtcg
    32281 ccgggtggca cgcgctcgac accccgccga tgtcccaccg gtcgcacgtg gcgaagagcg
    32341 ggagcatgcc gatcgccgca tgctctgcgg cgtgcaacgc gccgggcacg acctgggccg
    32401 gcaacgcctc ggccaccagg tcggcgggca ggatccacca caccccctcc gtcacgaact
    32461 cccgcaccgg caggtccagt tcgtgccggc cgatgatctc gccgcccggc agccgccgca
    32521 tgaaggagac gacctgggta tgcaccgtga cctgtccgcg gcatacggtc aggctccccg
    32581 acaactgcgc cgcatcgggg gtcgcgaagg tgaactccga gcgcgactgc gcttgcgtcg
    32641 accagcccgg gtccccggcg gcgacggtcg cgctcgcccg ttccaggtcg aggtgggtca
    32701 cgacatacgt ctgtccttgg tgcacgtgaa ctgcgcccgg gtggacctgc aggtcggcgg
    32761 aggcagagtc gatcgtgccg atgatccgcc cgcttgctcc ttcgacgatg gcaaccacct
    32821 cgcctgccgt gcgcagcgac ccgaccgcgt ccgggcgggt gtcctgcggc cagaaccagc
    32881 cgcccgggcg gcggcgcagc tgtccggcgg cgacgagctc gtcgagcgcc ggccacatcc
    32941 ccggcccgaa gaccccctcg tccgcctcgg tgatgcgccg ctcgtacgcc gccgcgatca
    33001 ggtggggcgg cagcaagcgc gggtttcccg ggttgacgac ggtccgttcc accggttcgc
    33061 cgaagatcgc ctccggatgc ttcatgagat aggcatcgag cgggtcgtcg gcggccacga
    33121 ggacggccag cgccgcggac ccgcggcgcc ccgcccgtcc ggcttgctgc cacaacgacg
    33181 acaaccgtcc gggccacccg gccatgacca ccgcgtccag gccgtggata tcgatcccca
    33241 gctcgagagc attggtcgcc gccaggcccc gcagagtccc ggcgcgcagg tcccgttcga
    33301 gcgcccgccg ctcctccggc aggtagccgc cgcggtaggc cgcgacccga tgcgacgact
    33361 ccgccgccgc gagcgaccgg ttcgccgccg ccgcaaccgc ctcgaccccg gcgcgcgagc
    33421 gggcgaacgc cagcgtctgc acgccgccgc cgacgagccg ggacagaagg cgggcgctct
    33481 cggtcgaggc cgccggccgg tgcgggcggc cgtgctcgtc gacatactcc tgcggctccc
    33541 acagggcaaa ggtcaacgca ccacggggcg aaccgtcgtg ggtgacctcg gccaccggca
    33601 ggcccaccaa gtcgctcgcg tgcgcccccg gctcgcccgc ggtcgccgag gcgaacacga
    33661 acacgggcgc actgcgatag cgctccagca ctcgccgcag ccggcgcagc acgagcgcca
    33721 cgtgcgagcc gaagatcccg cgataggcgt gcgattcgtc gatgaccacg atcgacaggg
    33781 ccctcaggaa ggaggcccag ccggcgtgcc ctggcagcag agagtggtgg accaggtccg
    33841 ggttggtcag cacgatgttg gcgtggtcgc ggatccaccg ccgctcctcg ggcgccgtgt
    33901 caccgtcata ggtggcggcc cgcacgccgg gcagcgcgag ccgcgtgatg tggtcggcct
    33961 ggtcggcggc gagcgccttg gtcggcgcga ggtagagcat cgtcgccccg cggccgttgg
    34021 gtgcccgggt gccgtcgagg acggcggtca gcccgggcag gaggtacgcg agtgacttgc
    34081 cggacgccgt gccggtggcg agcaccgtat gccgcccgga gcgcaccagt tccgcggcaa
    34141 gagactgatg gctccacaac gcgtcgatcc cggcgccggt caacgcggcg gccagtgccg
    34201 ggttgatcca ctcgggcatt gcgctcgtcg caccggtccg actcgggaag gagcggacgt
    34261 gcagaagcgg cccatggggc gctacacccg agagggtgga caaaatgtcc acgggggcct
    34321 gccccgaatg ggcaataggg gcgtgacctg cggtgtcgtg tggtgctgat gtcatctgtc
    34381 gtctacggta agcgcggcgg tgaccacgcg gtcaccagac tcccagctaa ggaagtgcta
    34441 g
//
LOCUS       DAOPJG010000012        34379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1335_length_34379_cov_7.321553, whole genome shotgun sequence.
ACCESSION   DAOPJG010000012 DAOPJG010000000
VERSION     DAOPJG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 34379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34379
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1335_length_34379_cov_7.321553"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKU75_02470"
     CDS             <1..402
                     /locus_tag="PKU75_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79770.1"
                     /translation="PGTGAVAMCTSRTQNVRDKYGTPAELPSIAHQNEYDATSATEPN
                     YRITCVFVDKRFRRQGVTELAITGALDLIAQAGGGRVESYPHDLSEQTKKMSSSFLYN
                     GTRRLYERLGFTYDRPKGLKNFVMVKAVEPR"
     gene            complement(419..1000)
                     /locus_tag="PKU75_02475"
     CDS             complement(419..1000)
                     /locus_tag="PKU75_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPF79771.1"
                     /translation="MPARFVYWMNVSLDLKIERRANEQGGGDWLRIGESLHRDFNERA
                     GTLSMMVQGRRIYETMEGFWPTARKNDGLPDYLREYGTIWTDIPKILVSRTRTTAGHN
                     TEIYGDDAMEQLAALRAGGEGDIGVGGASLASQLVTSGLLDELVLYVHPVVLGEGRPL
                     FDEGTPSVECDVLEQRSFDGGVTLHRYAVRTRQ"
     gene            1074..1595
                     /locus_tag="PKU75_02480"
     CDS             1074..1595
                     /locus_tag="PKU75_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF79772.1"
                     /translation="MSTPRSHCPINAAVEVFGDPWALLVLRDIMFGNRRHFRELQTGS
                     DEGIASNILSSRLKTLTQAGLLTREEAGRGRRAAYSLTEAGIQALPVMVALGTWGLAH
                     REGERQWRIRAEIMRDGGPAMTTALMDELREAHLGIPRPDPNAPRASEQLQAAFDDAI
                     KDLAATGPRVTRR"
     gene            complement(1582..3402)
                     /gene="glmS"
                     /locus_tag="PKU75_02485"
     CDS             complement(1582..3402)
                     /gene="glmS"
                     /locus_tag="PKU75_02485"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPF79773.1"
                     /translation="MCGIVAYIGDQPAAPFLIEGLKTLEYRGYDSAGLAVLGAKGTTA
                     VRSVGRIRDLEAALPKRLAGKVGIGHTRWATHGPATEANAHPHRSEDGRISVVHNGII
                     DNSGMLRDRLTDVGVTLASDTDTEVIAHLVARSTADTLEAAVLDALGQISGTYALAVM
                     DEAHPDRIVLARNGSPLIIGVGEKETLVASDAAALVRHTSQVIHLDDGELATVTAGGF
                     HTFDRNAEATGRQPVEIEVAAAEWELTGHQHYMYKEMLEQPDKAAAVIRGRLDERFAT
                     ARLNGLNLSPKELRAFRRVKILGCGSAYYVGQIGAQLIEDLARIPADAEAASEFRYRN
                     PIIEPETLYVVVSQSGETADTAFAVSEIKRKGGTVVAVVNAVGSTIAREVHGGIYLHA
                     GPEIAVASTKALTNMAIAFAMLALHMGRLRDLSVADGERILHALKRIPEQIQQVLDNE
                     PAIAEVAKALAQAQNAFYIGRVRQYPVAREGAQKLKEISYVHAEAYPTSELKHGPLAL
                     IDESMITVAIVPDDDLTERNVGAVQQVLARGGQVVALAHESVDLQGLDVERIDVPKSE
                     PELDPILMVIPTQLLAYHAALTLGYDIDKPRNLAKSVTVE"
     gene            complement(3468..3728)
                     /locus_tag="PKU75_02490"
     CDS             complement(3468..3728)
                     /locus_tag="PKU75_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79774.1"
                     /translation="MPDRKLVERVAASTGLSPGEAARVVDDVLAWYGEPVEAFVRRRH
                     ADLQLHGVRNAEAFARIAAELRRRVVAAPELTERQLRRLVYG"
     gene            3867..4571
                     /locus_tag="PKU75_02495"
     CDS             3867..4571
                     /locus_tag="PKU75_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79775.1"
                     /translation="MSRFLGGVAKVLGWFMALVVGGYVVVNGALVVKAQFKRNDVGGR
                     LTDKLAAAVPAATSHRDALTAAAGTAPKHAWIVQVCDFPTTDSGWMVNSYNQECELRS
                     AVAFAVATPEAAEALARQLPDELGGAVVEDIDTDGACRTIRTSGRPYSEEVQVRTLAI
                     TRRTGSEGWCGRLSTTGQHRVVSGEVRPLDANSDWVVVLRDQPLDRTDIGCLRWSVLF
                     CDNPFFDKPAWGDLPK"
     gene            complement(4578..6200)
                     /locus_tag="PKU75_02500"
     CDS             complement(4578..6200)
                     /locus_tag="PKU75_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF79776.1"
                     /translation="MRTIVVGGVAGGMSAATRLRRLEPEMEIVVLERSGHVSFANCGL
                     PYYIGGVIEKRSALLLQTPSSLAARFGLDVRVRHEVVAIDRAARTVTVRDLDGGTEDS
                     LTYDWLVLSPGARPVRPPIPGIERALSLRDIEDTDALAAAAQGATRAVVIGGGFIGVE
                     MGENLVHAGVEVSLIEATAQCMAPLDPEMVEPVHARMRSAGVDLRLQGRVVSIEEEGV
                     TLDGGERVAADLVVAAIGVQPDTQLARMAGLAVGERGGIVVDEHCRTSDSAIFGVGDA
                     VEKRDAGDGSSVLVPLANTANLQGRRVADVIAGRAVEVRPVQGTAIVGVFGLQVASTG
                     WNEKRLRSAGRAYRAIHTHPADHAGYYPGASTMALKLLVDAETDAILGAQGVGQAGVD
                     KRIDVIATAMRGGLGASDLADLELAYAPQFGSAKDPVNMLGYVAENLATGLTENIQWH
                     ELQDALDAGATLIDVRTAKEHAAGAIPGAVLIPVDELRDRACEIVSDDVVVHCAVGVR
                     GHVAAMMLARPGRRLRNLDGGYMTWKAGETTQ"
     gene            6287..8518
                     /gene="treS"
                     /locus_tag="PKU75_02505"
     CDS             6287..8518
                     /gene="treS"
                     /locus_tag="PKU75_02505"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009269 - response to desiccation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HPF79777.1"
                     /translation="MTDTTLPPDEILDPSDAVPEEVSFEGHSFPARPTRLRPRARLRD
                     ILWQRKAKQPGTASGDNPEYVAWLESESMLADARQFARKFAGQGSMWQNPYAAPDPRA
                     AIEKAPVWFTAYPISMITPAGATFLGTLGSTQLWDAFQQIGITAIHTGPVKRAGGVTG
                     WDTTPSVDGHFDRISTQFDDAFGTEDEFRAMCEVATDHGGLVIDDIVPGHTGKGADFR
                     LAEMKIGDYPGIYHMVEIPREDWHLLPKVPKGRDSANLDAETEKSLTDAGYIVGEMQR
                     VIFFEPGVKETNWSATDAVLGPDGEEHRWVYLHYFKEGQPSLNWLDPTFAGMRLVIGD
                     ALHSLGDLGSGALRLDANGFLGVEKSATGGPAWSEGHPLSEAANQLIASMVRKVGGFT
                     FQELNLTIDDIHSTSERGADLSYDFVNRPAYHHALAMEDAEFLRLTMRTAREMGVDTV
                     SLVHALQNHDEMTYELVHFATLHAGDTFMFRRKPIRGADLATLIRAELTEKIAGETGT
                     YNALFTTNGIASTLASVISASLGMADLSALTNDDVEAIKRAHLLLAMYNAWQPGVFAL
                     SGWDLVGALPVERKQVAALIRDGDTRWIHRGAYDLLDFQPNASESTSKMPKARALYGP
                     LNVQLEDPNSFVSRLREILAVRDRHGLATAQQVDIPAVSNKAMLVLVHRLAEADQVTV
                     LNFSAQTISGSVRSDELQPGSKVIDMVTGAPVGEVDDLHSLYVVLQPHEGRSLLVTPP
                     VEH"
     gene            complement(8496..8879)
                     /locus_tag="PKU75_02510"
     CDS             complement(8496..8879)
                     /locus_tag="PKU75_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPF79778.1"
                     /translation="MTAKTTPKATLPLTEPAAAGSCNTVLLRGRVSAVEETRVMPSGD
                     ELRTLRLVVPRVPVKGRKATVDTIDIACWTAATRRIAGRLGPGDEVAVTGSLHRRFFK
                     TPAGAASRYEVEAAEIRRVSAPPGE"
     gene            9118..10401
                     /locus_tag="PKU75_02515"
     CDS             9118..10401
                     /locus_tag="PKU75_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPF79779.1"
                     /translation="MRFLAGPGLIAASLTLALAGPASAAETSPGTESPSVSVTGSMTI
                     TIPPTGSTEAGLTKEELQRQIARAEALRAEILKGGADIAAAMTKLDKATAKANAALEA
                     YAKAKTEGRDARLKAKLQSETASNLQERLDKAREELREWAVSAYTQGGPLAEQLGYLD
                     ALSKDADKASNPLSDLNYLTDNRIRSVEDIREIAVAQKRASMDADAERDKAVAAEAKA
                     AKAKKIATDLVKDQQASVDEVRRLHQERIAEVAPIAGLLLGSGDEDAISTSERLTDAL
                     SEYDIAIKDLSTKPCTENTGVFPNGQIPPSHLCPMIGSSDEFLVPDAAAAFNAMSKAY
                     AEETGSLLCVTDGYRSYAEQVAVKASRGPWAATPGTSEHGLGRAVDLCGGVQDYSAPA
                     HLWLKQNAALFGWFHPSWAEQGGTLPEPWHWEYAG"
     gene            10483..11871
                     /locus_tag="PKU75_02520"
     CDS             10483..11871
                     /locus_tag="PKU75_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79780.1"
                     /translation="MTSAPAHVDSPDYAANAKYVRPWYWYDWANSAFVTTIGTVLYGP
                     YITTIAKQAACPGQDTDLACHTNLSLLGIPIAPGSLAPYTTTFATIVSAIFLIFIGAV
                     ADRSPRPARLLGIFAWLGALAAAGLFFVEGTRWQLGAILMVLATICLGSSLVIYDAIL
                     NDIAHPNDRDKVSSRGWALGYLGGGLLLALNLVLVTMKPFGLSTGMAVRISMLSAAVW
                     WGIFTLIPVLGMWNLRGVERPVTAVGEGAVGGSLKQLRNTFGDLRRHHPQTMLFLLAY
                     LFYNDGIQTVISSSSLYGAEELKFEQSQLIAVILLVQFVAFFGALLFGRLAGRHGAWR
                     TVLWSLALWTLIVIVAYFVPEKKFIPFIVLAVGIGLVLGGSQALSRSLYSQLVPKKRE
                     AEYFSLYQAMERGTSWFGTLLFGLVYQLTHTYRLAIIALIVFFVLGGILLSRVDVREG
                     IRQAGNEQPVLI"
     gene            complement(11999..12355)
                     /locus_tag="PKU75_02525"
     CDS             complement(11999..12355)
                     /locus_tag="PKU75_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HPF79781.1"
                     /translation="MADRTLRGSRMVSFSMETSENVVPSERQTVVYVCPQDHRIELPF
                     SIEAEIPATWECRCGAEALLLDGAAPDRKPVKPARTHWDMLLERRSIPELEELLEERL
                     TLLRQSRGEKPSKRSA"
     gene            complement(12549..13067)
                     /locus_tag="PKU75_02530"
     CDS             complement(12549..13067)
                     /locus_tag="PKU75_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HPF79782.1"
                     /translation="MSGSTPYAAGAAGSRRRPWRLWLLLALLIVPVLEIAAIIAVGKV
                     IGGWPTVALLLFESFLGAWLVRREGARTWKALNTALTTGAMPSRQLADAALVLIGGTL
                     LLTPGFLTDIVGFFFILPVTRPITRRLLEQAVRARLLGGAAAAAGAKFPGSRPGHRPA
                     SPDTVEGRVVDR"
     gene            complement(13095..13880)
                     /locus_tag="PKU75_02535"
     CDS             complement(13095..13880)
                     /locus_tag="PKU75_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPF79783.1"
                     /translation="MSDATGTREPVLRVLICIPTYNERENLPLVVAGVREHAPEADIL
                     VLDDNSPDGTGALADELAAADEHVHVLHRAGKEGLGMAYLAGFDWGLERGYDAIMEMD
                     ADGSHQPKYLPAMIAAIADADVVIGSRYVRGGSVVNWPASRKALSMGGNLYIRMILGM
                     GVRDATAGFRVYRASALRTIGLEQVASQGYCFQTDLTVRAVRAGLKIVEVPIEFVERE
                     IGASKMNNDIVKESMARVTRWGIEHRTGQVKKLVKREAQWEKL"
     gene            complement(13873..15501)
                     /gene="lnt"
                     /locus_tag="PKU75_02540"
     CDS             complement(13873..15501)
                     /gene="lnt"
                     /locus_tag="PKU75_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPF79784.1"
                     /translation="MSRDVPAPILPARLLLALAGGGAIALAFPDHNQWYAAWLGCGLI
                     AAAGWVARWRTGFLVGLVAGLAYFVPTLSWSGVFVGKLPWIALATLEALYVAAMVAVI
                     GPIQRRLVERRLGWAASATVPLGWVVGEWARSTTPFGGFPWARLAFSQADSPLRNLMS
                     LGGAPLLTAAVATLGVLLYAVARAALATRSRALTSRRRVLTSSRRVLVPAVAAVALAT
                     LPLAIPLAGTSSTTARVAIVQGNVPKAGLDFNAERRAVLDNHVRGTEALAATAGALDL
                     VIWPENAADIDPLRNTDAAAVITDARNAVKAPLLLGAILEEPAPLVSNASLLYRPGVL
                     DPERYVKQHPVPFAEYVPYKDFFRRFNSNVDLVRDGMATGTQVGYFALQTRSGAPFAA
                     LPTICFEVAYDGLIREAVTHSPQVPSVLVVQTNNATFGYTAESEQQFAISRLRAIEHN
                     RPVAHVSTVGVSGFIRPDGSYADKSSLYTAYRAVADLPLGQGTTIADRLGRIPEYTAL
                     AGLSLAWGLGRGPRRRDNGPSTTRSSSAKEATRV"
     gene            15621..15983
                     /locus_tag="PKU75_02545"
     CDS             15621..15983
                     /locus_tag="PKU75_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79785.1"
                     /translation="MLMLFITQAVQLLLVSLLVFGFFVLFGSLVIRPSVIEAWTGSAP
                     HYTGPLHLISDALFSVAVFLSGFAGLYFTVQAVIDQNYRKEFFRRIEDDLQQAIGVRK
                     VYTTLRTHLAPGAGTSRF"
     gene            15993..16679
                     /locus_tag="PKU75_02550"
     CDS             15993..16679
                     /locus_tag="PKU75_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF79786.1"
                     /translation="MSDAVDVLMVDDESALVASTLEYLQAFGLTTAGVGDAEAALAWL
                     TRHTVRVLLLDVNLPGMSGFDLCRRLRSTSQTPILFLSARAADDDEILALSLGGDDYL
                     RKPYSLGVLLAKIRRILQRSSAATDVPGYDDGHLRFDPAADRFWVDGVDLVLSAMEHR
                     LLRYLVLNRERIVPKDELFEQVWGDAITGDGTLSVHIRRLRTHIEPDPAQPRYIQTLW
                     GRGYSFRDLS"
     gene            16676..17959
                     /locus_tag="PKU75_02555"
     CDS             16676..17959
                     /locus_tag="PKU75_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPF79787.1"
                     /translation="MRRWLALLLLWAALGAAGLAVARASVPAAPPADVARANDLAQIA
                     AEHWPSLARNDFPSDAPPFAVVAADGRVVLDAGAGVRDDVSAYAARALGLDVVTDGGS
                     VARIYVVDPYADLLADRARRARDIALWSLAAAGLAATGWLWWLDRRILRPFRQLRGLA
                     GQVARGRLDAPLAVHRGDAFGAFSESFDILRRELARSREREAQAQQARKTLVSQLSHD
                     IRTPLATIHATSEVMQLSDPDPRLQVIMDKAGQIDALTRDLLAANATDAEDLGVTLTA
                     IGTPELRELIAAADAAGWVSECHLPDVLIVADRARLAQVLDNVFGNARKYAAPPVRVA
                     GALEDDLLTVQITDHGPGVPEGELALVTQRHYRAANAAGVQGQGLGLHTAYTLMDAMG
                     GALVVANAADGFSVALTLACAERQHDPRRHAFKTI"
     gene            18006..18788
                     /locus_tag="PKU75_02560"
     CDS             18006..18788
                     /locus_tag="PKU75_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79788.1"
                     /translation="MTQIQQQGTVLKASQISKTYAHDGLQQHILVNLDLDITRGEYVV
                     VMGPSGAGKSTLMHVLSGMDSPTLGEIEFDGAAIGALNPRRLTDFRRDHCGFVFQQIH
                     LLDSLSVLDNVLAVGLLGRRRRRAEVVRRAGELLDRVGIEEADRGKFPAMLSGGEAQR
                     VAVVRALINSPAVVFADEPTGQLNSESSSAVLDLLGEVSAGGQTVLMVTHDLRSALRG
                     DRVLYLRDGAIRGECVLGRRRDAEHDHDRLSRLTGFLDEMGW"
     gene            18791..>20717
                     /locus_tag="PKU75_02565"
     CDS             18791..>20717
                     /locus_tag="PKU75_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPF79789.1"
                     /translation="MSIRPASSFGVLALRNLRRARVQLAVVALLSLVAGALLNLGALC
                     GIRYPQLIRGEAERLDAPQIVLLTPTTEAARTQAYLRSDARVSQVDSMTVLSERASLR
                     LRSGSTAADVVYVDLDQQPALGRTKILERGDATVQDPVYLPHLFHATGDYGIGDPFTV
                     TTPAGLTRTFHVAGFFENVYLGFITMGATGLGLPHDEYVALGNASTPPDRLAFLQAKV
                     PDRKQITPVTADLTARLSAPSGAGHRSWVWNWDGIQIVTMTGPMLYALSLVLFALVVT
                     LIVLMAIGFWIRGAIDQDLPAIGMLETFGFRSGQIVRGLALPAALVVLLGATVGVVAS
                     YAAVPLIAESLAGQTGMPWHPRPDVVSAVVIVLMLTLAALVPGMLAARSLRRLEPVAA
                     LRNRIASRTFRRNWLPLSAARGNLQMHLGLKQALANRTQSILVAAVCLVVGLGSIFSA
                     SLFTNVLSNKENFMHMLVGDYGQVAARIAPDADPDAVLAEVRAIPGVQKAVLKDFLQG
                     SAANTQSLAVVMDDFEGQTFSSVAEGREPRHANEIAVGAVLARSTGKSVGQTISVRVG
                     STTADFLIVGTLSTVQYGGDRMDLTTAGYRRLVPNFQHEALDIAVQPGTSPTAVIDAM
                     SRRLDGRLSSVTNQQQDL"
     assembly_gap    20718..20817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20818..21095
                     /locus_tag="PKU75_02570"
     CDS             <20818..21095
                     /locus_tag="PKU75_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79790.1"
                     /translation="LQLMGQLIAAYVPLVTAGTAAGCLAGLVVVPRLLGSFLATVGVA
                     RASLDTSAAIVGSIGGGLVALAAMMVLLGAGPLLRREPVTLLAQRSG"
     gene            complement(21123..21506)
                     /locus_tag="PKU75_02575"
     CDS             complement(21123..21506)
                     /locus_tag="PKU75_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPF79791.1"
                     /translation="MSAPEIGMEVRHSRYVPYSHAHYAGNLVDGAYSLAAFGDVATEM
                     CIRTDGDEGLFASYSDVQFKAPVRAGDCIEIIARLVRAGTRSREMEFEVRVIARGNPE
                     RSESAADLLDPPIVATTARGVVVVP"
     gene            complement(21503..22363)
                     /locus_tag="PKU75_02580"
     CDS             complement(21503..22363)
                     /locus_tag="PKU75_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HPF79792.1"
                     /translation="MNPPPRTEAQETRNKSRQHVGQRRMEDALPGELSLHEESPLVRP
                     YGDTTGDGMVQISFTLPVPHDKRAEGAALQLAAKMGMSPAILVHAKSIAPSFTFFVVY
                     GAVTHLVDLREVEVVERDFDVLSARDTNALIRKRLRRKLVVVGACIGTDAHTVGIDAI
                     LNIKGFAGEKGLEYYREMAVHNLGAQVPIPELVNAAHDLRADAVLVSQVVTQKDAHIH
                     NTTAMSAAFREAFPAGKVPLLIVGGPRFEEGSAKELGVDRIFGRGTTPGEVASYLVHA
                     MIERPKGKSA"
     gene            complement(22360..23958)
                     /locus_tag="PKU75_02585"
     CDS             complement(22360..23958)
                     /locus_tag="PKU75_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HPF79793.1"
                     /translation="MVLRQRSVLELDPAEVKRARALARQAGRPVVNLAKRHTTVSVER
                     ATLRLAGLSGADHERVPWVNHLVDRVRQDVGLEHGVALPVWDALRRGDAPDLTTLAQK
                     AAGGAVTFRLPEGKDSTAAGKAARTAISRGVRTIDKQRATRDKLIARHGDPKQKPWIY
                     LIVATGDIYEDIPQAQAAAREGADIIAVIRSTGQSLLDFVPEGATREGYAGTYATQEN
                     FRLMRAALDETSKELGRYVRLTNYASGLCMPEIATLAGLERLDMMLNDSMYGILFRDI
                     NPIRTFVDQRFSRQIHARAGIIINTGEDNYLTTADAVEAAHTVTVSQLLNEYFAKEAG
                     LADWQLGLGHAFEIDPTVPDSFRLELAHALLARTLFPDAPLKWMPPTKHMTGDIFRGY
                     LLDGFFNLVGAMTGQGILLVGMMTEAVVTPWLSDRDLALQNVRYVLDAAGGLTEDFHP
                     PREGLIQTRARQVLREAIDLLQEIVDDAEETGDPALLAAIADGTFGAMKRPPTKGKGL
                     DGVARKAPGYLNPATDLLSEGSDR"
     gene            24195..24830
                     /locus_tag="PKU75_02590"
     CDS             24195..24830
                     /locus_tag="PKU75_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79794.1"
                     /translation="MPLLANVAASTLAAELTTLESTLSTPKLIAARVLSVSTRSAMLA
                     QLRQLRSAVGQVQAAAQHKVDTTRAALAGARAQAEAAARQSTQGAKDVARAAKDHQRA
                     EAKKAEAKRKAEATRKAEAKRKAEAKKQAVAEAKRKAEAEAAAKRAAEAKKAQVPATP
                     RSSTPAAPRTQQPTTPNVPANPGLTYTGCRAYGPNGIWLDAKGRRFSPIPC"
     gene            complement(24835..25128)
                     /locus_tag="PKU75_02595"
     CDS             complement(24835..25128)
                     /locus_tag="PKU75_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HPF79795.1"
                     /translation="MIVAFSVAPMTGGDSVSEQVAAAVRVVRESGLPNRTSSMFTEIE
                     GEWDEVMDVVKRATEAAGAGAPRVSLVLKADIRPGHTGELDGKVERVERRLAD"
     gene            25228..26946
                     /locus_tag="PKU75_02600"
     CDS             25228..26946
                     /locus_tag="PKU75_02600"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HPF79796.1"
                     /translation="MPTSDYEQDTSTGFGPLRIRARGRAVMTNPTINRGTAFTQPQRK
                     QLGLVGLVPSSVTTLENQVRRVYEQYRRTTTRLGKYLHLNGVRDRNEVLFYRLLTSNI
                     EEMLPIIYTPTIGEAIERFSHEFSRPRGVFLSIDHPEMMEESFLNFGIEPDEVDLVVV
                     TDSEGILGIGDQGIGGIQIAIGKLNVYTAAAGIHPRRCIPVVLDVGTDNLGLLSSEMY
                     LGERHARVRGEKYDQFLDRFVHTVTELFPNAMLHWEDFGTSNAHRLLTKYREEICTFN
                     DDIQGTAAVVLAAILAAVDVTGLPLDDHRVCVFGAGSAGIGIANLIRDTMLRTGASET
                     EEEAYARFYPIGINGLYVDDQPELLAFQRPYARTRAEVEHWAVADPAHITLEDVIRNV
                     KPTILIGTSTRGGAFTRGIVEEMSSYCQRPIIFPLSNPTSRAEATPADVLTWSDGRAL
                     VATGSPFGPVDYDGTRYTIAQANNALIFPGLGLGVATCRATRISEGMIAAAAEALASM
                     VNAYRQGAPLLPSISALRQVSATVAVAVAKQAEKEGLAREPLTDPIQQIYQRMWQPIY
                     PEIEAV"
     gene            27019..28086
                     /gene="mgrA"
                     /locus_tag="PKU75_02605"
     CDS             27019..28086
                     /gene="mgrA"
                     /locus_tag="PKU75_02605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HPF79797.1"
                     /translation="MTQLGIDYLADADRYEGRMPYRPVGRSGLRLPAVSLGLWHNFGD
                     DVPMERQRAILRRAFDLGVTHFDLANNYGPPYGSAERNFGEHLAADFRPYRDELIISS
                     KAGYDMWPGPYGQGGGMRKYLIASCDQSLRRMGLDYVDIFYSHRFDPDTPLEETMGAL
                     DYIVRSGRALYAGISSYSGPRTSEAVRILRELGTPLLIHQPSYSMLNRWIEEEPAEGG
                     EPLLDVLGMSGVGCIAFSPLAQGMLTNKYLKGIPADSRAAQDKSLSESLLTVDALKHV
                     AALNRIAKKRGQTLAQMAIAWVLRDPRVTSALIGASSVSQLEDSVAAVKNLDFSDAEL
                     KSIDRHAVDSGINLWAASSAG"
     assembly_gap    28135..28234
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28368..28592)
                     /locus_tag="PKU75_02610"
                     /pseudo
     CDS             complement(28368..28592)
                     /locus_tag="PKU75_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
     gene            complement(28770..29834)
                     /locus_tag="PKU75_02615"
     CDS             complement(28770..29834)
                     /locus_tag="PKU75_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HPF79798.1"
                     /translation="MSRPVSSPVGLHRVLEPAGESLPQAACRLDATSPLWPDEVRVAV
                     ETLNLDAASYRQLAESHTADGVLDAQALKAQVLDIVATRGKMQNPVTGSGGMLIGTVE
                     EVGPESTLGLAVGDRVATLVSLSLTPLVITDGLASWDGRSERVPAHGHAILFGRSIAA
                     KLPEDLSPELALMVMDVCGAPALVRRVVESYAARGAAPTVLVLGGAGKSGSLALAAAA
                     ASGGGRRIAVVPNEVEAERLRATRLADAVVIADARSPLGLSQAVAAAGGPADLTVVCV
                     DVPGCEQPAILSTAQGGTVIFFSMATNFAAAALGAEGLAADVTMLVGNGYVPGHAAYA
                     LDLLRADADVRAMFEARLSE"
     gene            29923..31383
                     /locus_tag="PKU75_02620"
     CDS             29923..31383
                     /locus_tag="PKU75_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPF79799.1"
                     /translation="MTAHVEQPYVYTQRELVEPDWTRFPGWRDVTPAQWEDAQWQRVN
                     CVKNIKQLRDLMGDLLQERFYADLERDQAERATMSMLVPPQMMNTMVAATADPMPAAG
                     ADFTAAFYADPVRLYMLPVFSDRRTDWPSHPYATRDSLHEHDMWAVEGLTHRYPTKVL
                     AEMLPTCPQYCGHCTRMDLVGNSTPQVAKLKLAGKPVDRYAAMLDYLQRTPQVRDVVV
                     SGGDVANMPWKNLEAFLDQLLRVDNIRDIRLATKALMGLPQHWLQPDVVEGIERVAKT
                     AHERGVSLAIHTHVNNTASVTPLVAKASRTMLEVGVRDVRNQGVLMRGVNDSADQLLD
                     LCFALQDGAMITPYYFYMCDMIPFSEHWRVSLAEAQHLQHAIMGYLPGFATPRIVCDV
                     PFVGKRWVHQVESYDRELGMSFWRKNYRTSIEAADTEAISRDYVYYDPIYSLPESGQQ
                     WWRSQGDHGADLEIAMARAAASRDAASRAADLLAVH"
     gene            31577..32152
                     /locus_tag="PKU75_02625"
     CDS             31577..32152
                     /locus_tag="PKU75_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPF79800.1"
                     /translation="MTAIVERPRVLADVIPGGLVRDAALVLGGAGFIGLLAQWAIPLG
                     FTPIPLSLGTFAVLLTGAALGAGRGLASVGIYVLAGMAGMPWFAEHRSGWGFPSFGYL
                     IGFILAATLVGALASRGGDRTVLKTIGLMVLGNLVIYAVGVPYLAANTGMDLSTAIEK
                     GLTPFLLGDGLKIAAAAGLLPGAWALVRRVR"
     gene            32158..32658
                     /gene="ybaK"
                     /locus_tag="PKU75_02630"
     CDS             32158..32658
                     /gene="ybaK"
                     /locus_tag="PKU75_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HPF79801.1"
                     /translation="MAKKSTHTSGTPATVALTAAGVRFTQHTYPHDPSAPSYGLEAAE
                     ALGVPPAQVFKTLLVDLEGGPDRTAVGIVPVTQQLDLKAMATACAAKRATMCDPVAAQ
                     RITGYVVGGISPVGQKRALPTLLDESAAAQAVVYVSGGRRGFDIGLAPAELLRVIVGR
                     YAAIAR"
     gene            complement(32667..33089)
                     /locus_tag="PKU75_02635"
     CDS             complement(32667..33089)
                     /locus_tag="PKU75_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPF79802.1"
                     /translation="MEDVDRRLVQLLRQDGRMSYTDLGKAVGLSTSAVHQRVRRLEER
                     KVLLGYGARVAHDQLGDPLTAFIAVTPFDLSAPDDIPERLRGISEIEACHSVAGDSNY
                     LLKVRVATPGALESLISRIRAAANVSTHTTIVLSTPWE"
     gene            33170..34102
                     /locus_tag="PKU75_02640"
     CDS             33170..34102
                     /locus_tag="PKU75_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HPF79803.1"
                     /translation="MTEPRLMLLDSASLYFRAFFGVPDDRPDDAPGPATNAVRGFLDF
                     IATLVREHHPTHLIACWDNDWRPQFRVDAIPSYKTHRVADGSVEEEEVPDALTPQVPI
                     IVDALAALGITRLGADGYEADDAIGTLVARHKGTMPIDVVTGDRDLFQLVDDAAHVRV
                     LYTARGGVRNADVVDEAFLMEKYGVANGEQYADMAVLRGDTSDGLPGVAGVGEKTAAK
                     LITAYGSLTALREAIDGGDPAIKGAQRRNLEAGSAYLDVGPLVVRVAQDAPVPDESGE
                     LPTEIADLAGMSRLASEYGLTSPINRVIDALGLG"
     gene            complement(34149..34286)
                     /locus_tag="PKU75_02645"
     CDS             complement(34149..34286)
                     /locus_tag="PKU75_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79804.1"
                     /translation="MLESTDLDVETIARRCGFGSSALLRHHFAPHVGVPPTDYRRTFA
                     R"
BASE COUNT         5372 a        11913 c        11461 g         5433 t
ORIGIN      
        1 cctgggactg gcgctgtagc gatgtgcacg tcacggacgc agaacgtccg ggacaagtac
       61 gggaccccgg ccgagctgcc atcgatcgcg caccagaacg aatacgacgc cacaagtgcg
      121 acggagccca actatcggat cacctgtgtc ttcgtcgaca agcgattccg ccgccaaggc
      181 gtgaccgaac tcgcgatcac cggggcgctg gatttgattg cgcaggccgg tggtggccgg
      241 gtcgagagct atccgcacga cctgagcgag cagacgaaga agatgtcatc ctcgttcctc
      301 tacaacggaa cccgccggct ctatgagcgt ctcggcttca cctacgaccg gcccaagggg
      361 ctcaagaact tcgtgatggt caaggccgtc gagccccgct gagacccggc cggctggatc
      421 actgacgagt gcggacggca taccggtgca gagtgacgcc accgtcgaag gaccgctgct
      481 cgagcacgtc gcactcgacg gacggcgtgc cctcgtcgaa cagcggcctg ccctccccca
      541 gcacgaccgg atgcacatag agcaccaact cgtcgaggag accggacgtc acgagctggg
      601 acgccagcga cgctcccccg acgccgatgt ccccctctcc cccggcccgc aatgccgcca
      661 gctgctccat cgcgtcatcc ccgtagatct ccgtgttgtg cccggctgtc gttcgcgtcc
      721 gggagacaag gattttgggt atgtcggtcc agatcgtgcc gtactcgcgc aagtagtcgg
      781 gaaggccgtc attcttgcga gcggtcggcc agaacccttc catggtctcg tagatgcgcc
      841 ggccctggac catcatcgac aacgtgccgg cgcgctcgtt gaagtcgcga tgcagcgact
      901 ccccgatccg cagccagtcg ccgccacctt gctcgttggc gcggcgttcg atcttcaggt
      961 cgagcgagac attcatccag tagacgaatc gtgcgggcat gatccctcct gatgtggttg
     1021 cgagttgcaa gcactgtcac cctatgcact actggtggta aattgcaagc actatgagca
     1081 cgccgcgttc gcactgcccg atcaacgccg ccgtggaggt cttcggcgat ccgtgggcgc
     1141 tgttggtgtt gcgcgacatc atgttcggca accgccgcca ctttcgggag ttgcagaccg
     1201 gcagcgacga gggcatcgcg agcaacatcc tcagcagccg tctcaagacg ctgacccagg
     1261 ccggcttgct gacgcgtgag gaggccgggc gagggcgccg ggcggcctat tcgttgacgg
     1321 aggccggaat tcaggccctg cctgtcatgg tggccctcgg gacctgggga ctcgcgcacc
     1381 gggagggcga gcgccagtgg cgtatccgcg ccgagatcat gcgcgacggc gggccggcca
     1441 tgacgacggc tctgatggat gagttgcgcg aggcccacct cggcatcccc cgcccggacc
     1501 cgaacgcccc gcgcgccagt gagcaactcc aggcggcctt cgacgatgcc ataaaagacc
     1561 tcgcggcaac cgggccaagg gtcactcgac ggtgacggac ttggcgagat tgcgcggctt
     1621 gtcgatgtcg tagccgaggg tcagtgccgc gtgataggcc agcagttgcg tgggaatcac
     1681 catgaggatc gggtcgagtt ccggctccga cttcggcaca tcgatgcgct cgacatcaag
     1741 gccctgcagg tcgacggact cgtgcgccag ggcaaccacc tgccccccgc gcgccagaac
     1801 ctgttgcacc gcaccgacat tgcgctcggt cagatcgtcg tcgggcacga tggccacggt
     1861 gatcatcgac tcgtcgatca gtgcaagcgg tccgtgcttg agttcgctgg tcggataggc
     1921 ctccgcgtgc acatagctga tctccttgag cttctgcgcg ccctcacgag cgaccggata
     1981 ctgccgcacg cggccgatat agaaagcgtt ctgcgcctga gccagcgcct tggcgacctc
     2041 ggcgatcgcc ggctcgttgt cgagcacctg ctggatctgc tcgggaatcc gcttgagcgc
     2101 gtgcaggatt cgttcgccat cggcgaccga gaggtcacgc agccggccca tgtgcagtgc
     2161 cagcatcgcg aaggcgatgg ccatattcgt caaggccttc gtggacgcga ccgcgatctc
     2221 ggggccggcg tgcaggtaga tcccgccgtg cacctctcgc gcgatggtcg acccgacggc
     2281 gttgaccacg gccacgaccg tcccgccctt gcgcttgatc tccgacacgg cgaaggccgt
     2341 gtcggccgtc tcgccggact gggagacgac gacatacagc gtctcgggct cgatgatcgg
     2401 gttgcgatag cgaaactccg aggcggcctc ggcgtccgcc gggatccggg cgaggtcttc
     2461 gatcaactgg gcaccgatct gcccgacgta ataggccgac ccgcagccga ggatcttgac
     2521 tcgccggaag gcgcgcagtt ccttgggcga gaggttgagc ccgttgagcc gggccgtggc
     2581 gaagcgttcg tcgagccggc cgcggatgac ggccgcggcc ttgtccggct gctcgagcat
     2641 ctccttgtac atatagtgct ggtgcccggt cagctcccac tcggccgccg ccacctcgat
     2701 ctcgaccggt tgccggccgg tcgcctcggc gttccggtcg aaggtgtgga acccgccggc
     2761 ggtgacggtc gccagttcgc cgtcgtcgag gtgaatgacc tgcgaggtat gccgcaccag
     2821 cgccgcggcg tcgctcgcga cgagcgtctc cttctctcca accccgatga tgagcggaga
     2881 accgttgcgg gccagcacga tccgatccgg gtgcgcctcg tccatcaccg ccagcgcata
     2941 ggtgcccgag atctgcccga gcgcatcgag gaccgcggcc tcgagcgtgt cggccgtgga
     3001 gcgcgccacg agatgcgcga tgacctcggt gtcggtgtcg ctggcgagtg tcacgcccac
     3061 atcggtcagc cggtcacgga gcatcccgga gttgtcgatg atcccgttgt gcaccacaga
     3121 gatccgcccg tcctcgctgc ggtgcgggtg ggcgttggcc tccgtggccg ggccgtgcgt
     3181 ggcccatcgg gtgtgcccga tgccgacctt cccggcgagt cgcttcggga gggctgcctc
     3241 caggtcgcgg atgcgtccca ccgaccgcac ggctgtcgtg cccttggcgc cgagcacggc
     3301 gaggcccgcg ctgtcgtagc cgcgatactc cagcgtcttc agcccctcga tgaggaaggg
     3361 ggcggccggc tggtcgccga tataggcaac gattccgcac atggggttgg actcctcgtg
     3421 atcagtggtg gtggtgatgg tcgggggcgg tcagcgggta tgccgcgtca gccgtagacg
     3481 aggcggcgca gttgtcgttc cgtcagttcg ggcgcggcga cgacgcgtcg ccgcaattcc
     3541 gcggcgatgc gggcgaaggc ctcggcgtta cgcactccgt gcaactgcag atccgcgtgc
     3601 cggcggcgca cgaaggcctc caccggctcg ccataccagg ccaggacgtc gtcgacgacc
     3661 cgcgccgcct cccccggact caggcccgtc gaggcggcga cccgctcgac caacttccga
     3721 tccggcatac ccccaatcat cctgccgcgg agcccgaaaa ccaaaaatct gcccgaattc
     3781 gggcaaatca cgctacttgt ctctccacgc gagatgatta cagcgcctca tgccgcgcag
     3841 gcggaatgtc ccctagcgtc caggacatgt ctcgttttct cgggggtgtc gccaaggtcc
     3901 tcggctggtt catggcactc gtggtcgggg gctacgtggt cgtcaacggc gccctggtcg
     3961 tcaaggccca gttcaagcgc aatgacgtcg gcggccggct caccgacaag ctcgccgccg
     4021 cagtgccggc cgcgacgagc caccgcgacg ccttgaccgc ggcggccggc acggcaccga
     4081 agcacgcgtg gatcgtccag gtctgcgact tcccgaccac ggacagcggc tggatggtca
     4141 attcgtacaa ccaggagtgc gaactgcggt ccgccgtggc gttcgcggtc gcgacacccg
     4201 aggccgcgga ggcgctggcc cgccagttgc ccgacgagct tggcggagcc gtcgtcgagg
     4261 acatcgacac cgatggcgcg tgtcgcacca tccgcacgtc gggtcgcccg tacagcgaag
     4321 aagtccaggt gcggaccctt gccatcactc gacgcaccgg ctccgaaggc tggtgcggcc
     4381 ggctgtcgac gaccgggcag caccgcgtcg tctccggtga ggttagaccc ctcgacgcga
     4441 actcggactg ggtggtcgtg cttcgggacc agccgctcga ccgcaccgac atcggctgcc
     4501 tgcgctggtc ggtcctgttc tgtgacaacc cctttttcga caagcccgcc tggggcgacc
     4561 tgccgaagta agggcgctta ctgcgttgtc tcgccggcct tccaggtcat gtagccgccg
     4621 tccaggttgc gcagccggcg ccccggacgc gcaagcatca tcgcggccac gtggccgcgg
     4681 acgccgaccg cgcaatgcac cacgacgtca tccgagacga tctcgcaagc gcgatcgcgc
     4741 agttcgtcca cggggatcag gaccgcgccg gggatggcgc cggccgcgtg ctccttcgcc
     4801 gtgcggacat cgatcagtgt cgcgcccgcg tcgagggcgt cctggagctc gtgccattgg
     4861 atgttctcgg tcaggccggt ggcgagattc tccgcgacat agccgagcat gttcaccgga
     4921 tccttggccg acccgaactg cggggcgtag gcgagttcca gatcggcgag gtcactcgcc
     4981 ccgaggcccc cgcgcatcgc cgtcgcgatc acgtcgatcc gcttgtcgac gccggcctgc
     5041 cccacgccct gcgcgccgag gatggcgtcg gtctcggcat ccaccagcaa cttcagggcc
     5101 atcgtcgacg cgccgggata gtagccggcg tggtcggcgg gatgggtgtg gatggcccga
     5161 taggcccggc cggcggaccg cagccgcttc tcgttccagc ccgtgctcgc cacttgcaga
     5221 ccgaagaccc cgacgatcgc cgtgccctgc accggacgca cctccacggc ccgccccgcg
     5281 atgacgtcgg cgacgcgccg cccctgcagg ttcgcggtgt tggccagggg cacgagcacc
     5341 gacgagccgt cgccggcatc gcgcttctcc accgcgtcac cgacgccgaa gattgcggaa
     5401 tcgcttgtgc ggcaatgctc gtcgacgacg atgccgcccc gctcccccac cgcgagcccg
     5461 gccatcctgg ccagttgcgt gtcgggttgc acgccgatcg ccgccaccac gagatcggcc
     5521 gccacgcgct caccgccgtc gagggtcacg ccctcctcct cgatcgacac cactcgcccc
     5581 tgaaggcgca gatccactcc ggcagaacgc attcgggcat gcaccggctc gaccatctct
     5641 gggtcgagcg gcgccatgca ttgagctgtc gcctcgatga ggctcacctc gacacccgcg
     5701 tgcacgaggt tctcccccat ctccacgccg atgaacccgc ccccgatgac caccgcccgg
     5761 gtcgccccct gggcagcggc ggcgagcgcg tcggtgtcct cgatgtcccg cagactcagc
     5821 gcccgttcga tgcccgggat cggcgggcgt accggccgcg cacccggaga gaggaccagc
     5881 cagtcatacg tcagcgagtc ctcggtcccg ccgtcgaggt cgcgcaccgt gaccgtgcgc
     5941 gctgcgcggt cgatggcgac gacctcgtgg cgcacgcgca cgtcgagccc aaagcgcgcc
     6001 gccagcgacg acggcgtctg cagcaggagc gccgaccgct tctcgatcac accgccgatg
     6061 tagtacggca gaccgcagtt cgcgaacgac acatggccgc tgcgctcgag gacgacgatc
     6121 tccatctcgg gttcgagacg gcgcaaccgg gtcgcggcgg acatgccacc cgcgacccct
     6181 ccgacgacga tggttcgcat gtccccaaaa taccccgctg ggtatggtgt ctgcgccggt
     6241 cgggatgtga cacggccaag cgcgcggatc cgtaccatag tccctcgtga ccgacaccac
     6301 actgcctccc gacgagatcc tcgaccccag cgacgccgtg ccggaagagg tcagtttcga
     6361 aggtcacagc ttcccggccc gccccacccg gctgcgtccg cgcgcgagat tgcgcgacat
     6421 cctctggcag cgcaaggcca aacagcccgg caccgcgagc ggagacaacc cggagtatgt
     6481 cgcgtggctg gagtcggagt cgatgctcgc cgacgcccgg cagttcgcac gcaagttcgc
     6541 cggccagggc agcatgtggc agaacccgta tgccgccccg gatccccgcg ccgccatcga
     6601 gaaggcgccg gtctggttca cggcctaccc gatctcgatg atcaccccgg ccggggcgac
     6661 cttcctcggg acgctcggca gcacgcagct gtgggatgcg ttccagcaga tcggcatcac
     6721 cgccatccac accggcccgg tcaagcgcgc cggcggcgtc accggctggg ataccacccc
     6781 cagcgtcgac ggtcacttcg accgcatctc gacccagttc gacgacgcct tcggcaccga
     6841 agacgagttc cgggcgatgt gcgaagtggc cactgatcac ggcgggctgg tcatcgacga
     6901 catcgtccct ggccacaccg gcaagggggc ggacttccgg ctcgccgaga tgaagatcgg
     6961 cgactacccc ggcatctatc acatggtcga gatcccgcgg gaggactggc acctgctgcc
     7021 caaggtgccg aagggccgcg actcggccaa cctcgatgcc gagaccgaaa agtccctcac
     7081 cgatgccggc tacatcgtcg gggagatgca gcgggtgatc ttcttcgaac ccggggtcaa
     7141 ggagacgaac tggtcggcca ccgacgcggt cctcgggccc gacggcgagg aacatcggtg
     7201 ggtctacctg cactacttca aggagggcca gccgtcactg aactggctgg accccacctt
     7261 cgccggcatg cggctggtca tcggtgacgc gttgcactcg ctcggcgacc tcggctccgg
     7321 cgcgctgcgt ctggacgcca acggcttcct cggcgtcgag aagtccgcga ccggtggccc
     7381 ggcgtggtcg gagggccacc cgctctccga ggcggccaac cagctgatcg ccagcatggt
     7441 ccgcaaggtc ggcggcttca ccttccagga actcaacctg accatcgatg acatccactc
     7501 cacgtccgag cgcggcgccg acttgtccta cgacttcgtc aaccgaccgg cataccatca
     7561 cgccctcgcg atggaggatg cggaattcct ccggctgacg atgcgcaccg cccgcgagat
     7621 gggcgtcgac accgtctcgc tcgtgcacgc cctgcagaac cacgacgaga tgacctacga
     7681 gctggtgcat ttcgccaccc tgcatgccgg cgacaccttc atgttccggc gcaagccgat
     7741 ccgcggcgcc gaccttgcga ccctcatccg ggccgaactc accgagaaga tcgccggcga
     7801 gaccggcacc tacaacgccc tgttcaccac caacggcatc gcctccaccc tcgcttcggt
     7861 catctccgcc tccctcggca tggccgacct gagcgccctg accaacgacg acgtcgaggc
     7921 catcaaacgc gctcacctgc tgctcgcgat gtacaacgcg tggcagccgg gtgtcttcgc
     7981 cctctccggc tgggacctcg tcggcgccct gccggtcgag cgcaagcagg tggcagcgct
     8041 gatccgcgac ggtgacaccc ggtggatcca ccgcggcgcc tacgacctcc tcgatttcca
     8101 gccgaatgcg agcgagtcga cctcgaagat gccgaaggcc cgcgccctgt acggcccgct
     8161 gaacgtgcag ctcgaggacc cgaactcctt cgtctcgcgg ctgcgcgaga tcctcgcggt
     8221 ccgcgaccgg catggtctcg ccactgccca gcaggtcgac atccccgccg tctcgaacaa
     8281 ggcgatgctc gtgctggtcc accgtctcgc cgaagcggac caggtcaccg tgctgaactt
     8341 ctcggcgcag acgatctccg gcagcgtccg ctccgacgag ctgcagccgg gcagcaaggt
     8401 catcgacatg gtcacgggtg cgccggtggg cgaggtcgac gatctgcatt cgctgtatgt
     8461 cgttctgcag ccccacgagg gccggtcgct gctcgtcact cccccggtgg agcactgacc
     8521 cggcgaatct ccgccgcctc cacctcatac cgactcgcgg cgcccgcagg cgtcttgaag
     8581 aaccgccggt gcagcgaacc cgtcacggca acctcgtcgc ccggccccag gcggccggcg
     8641 atccgccggg tcgccgcggt ccagcaggcg atgtcgatgg tgtcgacggt ggccttgcgg
     8701 cccttgaccg ggacacgcgg cacgaccaga cgcagggtgc gcaattcgtc gccgctgggc
     8761 atgacccggg tctcttcgac cgcgctgacg cggccgcgga gcaggacggt gttgcagctc
     8821 ccggccgccg ccggttcggt cagcgggagg gtggctttgg gcgtggtctt ggctgtcatg
     8881 accccacgct cccccgccgg cccgctgctg ccaagacccc tcgcgtgcac ctgtggacgg
     8941 cgcgaattcg ccctttcggc tgtggacaac cggccgtgtc aacaaccaca ggccgggggg
     9001 ttgggccgct tggaagatca cgatacgatt gcggccggat cagtgtttct gggccgtggc
     9061 ctaggcatgt gtccgcaggt gcgcaaccgg tgcgcttgaa cggatttcgg agttgatgtg
     9121 cgcttcctgg ctggccccgg tctcatcgcg gcgtccctca cgctcgccct ggccggtccc
     9181 gcctccgccg ccgagaccag ccccggcacc gagagcccat ccgtgtccgt caccgggtcg
     9241 atgacgatca cgatccctcc gaccggcagc accgaggccg gcctgacgaa ggaagaactc
     9301 caacgccaga tcgctcgtgc cgaagcgctg cgggccgaga tcctcaaggg cggagccgac
     9361 atcgccgccg ccatgaccaa gctggacaag gcgacggcca aggccaacgc ggcactcgag
     9421 gcctacgcca aggccaagac cgagggccgc gatgctcgcc tgaaggccaa gctgcagtcc
     9481 gagaccgcca gcaacctcca ggagcgtctc gacaaggccc gcgaagagct tcgggaatgg
     9541 gccgtcagcg cctacaccca aggcggaccg ctcgccgaac aactcggcta cctcgacgcc
     9601 ctctccaagg acgccgacaa ggcctccaac cccctctccg acctcaacta cctcaccgac
     9661 aaccgcatcc gctcggtcga ggacatccgc gagatcgcgg tcgcgcagaa gcgcgcctcc
     9721 atggatgccg acgccgagcg cgacaaggcg gtggccgccg aggccaaggc ggcgaaggcc
     9781 aagaagatcg ccaccgacct ggtcaaggac cagcaggcta gcgtcgatga ggtgcgccgg
     9841 ctgcaccaag aacgcatcgc cgaggttgcg cccatcgccg gcctgctgct gggcagcggc
     9901 gacgaggacg ccatcagcac cagcgagcgc ctcaccgacg ccctcagcga atacgacatc
     9961 gcgatcaagg acctgagcac caagccgtgc accgagaaca ccggtgtctt ccccaacggg
    10021 cagatccccc cgtcacacct gtgcccgatg atcggctcca gtgacgagtt cctggtgccc
    10081 gatgctgccg cggccttcaa tgcgatgtcc aaggcctacg ccgaagagac cggctcgctg
    10141 ctgtgcgtca ccgacggtta ccgctcgtat gccgagcagg tcgccgtcaa ggccagccgc
    10201 ggcccgtggg cggccacccc gggcaccagc gagcacgggc tcggccgcgc cgtcgacctg
    10261 tgcggtggcg tccaggacta cagcgccccg gcgcacctgt ggctgaagca gaacgccgca
    10321 ctcttcggct ggttccaccc ctcctgggcc gaacagggtg gcacccttcc cgagccctgg
    10381 cactgggaat acgcaggctg accgcggccg ttccgcaccc ggcttactgc tgagtaggtg
    10441 cgcctatgcc gctcggggcg cgcggtgtgc cagagttccg gcatgacttc cgcgccggcg
    10501 cacgtcgact caccggacta cgcggcgaac gccaagtacg tccggccgtg gtactggtac
    10561 gactgggcca actccgcctt cgtcaccacc atcggcaccg tcctctacgg cccctacatc
    10621 acgaccatcg ccaagcaggc ggcctgcccg ggacaggaca ccgacctggc gtgccacacc
    10681 aacttgagcc tgctcggcat accaatcgcc cccggctcgc tggcgccgta cacgacgacc
    10741 ttcgccacga tcgtctcggc gatcttcctc atcttcatcg gcgcggtcgc ggaccgctcc
    10801 ccacgcccgg cgcgcctgct cgggatcttc gcgtggctgg gcgcgctggc tgccgccggg
    10861 ctcttcttcg tcgagggcac ccgctggcag ctgggcgcca tactcatggt gttggcgacc
    10921 atctgcctgg gctcctcgct ggtcatctac gacgcgatcc tcaacgacat agcccatccc
    10981 aacgaccggg acaaggtctc ctcacgcggg tgggcgctcg gctatctggg cggcgggctg
    11041 ctgctcgcgc tgaacctggt cctggtgacg atgaaaccct tcgggctgag caccggtatg
    11101 gccgtgcgga tcagcatgct gtccgccgcc gtgtggtggg gcatcttcac gctcatcccg
    11161 gtgctgggga tgtggaacct gcgcggcgtc gagcgcccgg tcaccgcggt cggcgagggc
    11221 gccgtcggcg gcagcctcaa acagttgcgc aacaccttcg gggacctgcg ccgccaccat
    11281 ccgcagacga tgctcttcct gctcgcctac ctcttctaca acgacggcat ccagaccgtc
    11341 atctcctcct cgagcctcta cggcgccgag gagctgaagt tcgaacagtc ccagctgatc
    11401 gccgtcatcc tgctcgtgca attcgtcgcc ttcttcggcg cgctgctctt cggcagactg
    11461 gcgggccggc acggcgcctg gcgcaccgtc ttgtggtccc tcgcgctgtg gaccctcatc
    11521 gtgatcgtgg cctacttcgt cccggagaag aagttcatcc cgttcatcgt gctggccgtc
    11581 ggcatcggcc tcgtcctcgg cggctcccag gcgctctcgc gcagcctcta cagccagctg
    11641 gtccccaaga agcgcgaagc cgagtacttc agcctctacc aggcgatgga gcgcggcacc
    11701 agctggttcg gcaccctgct cttcgggctc gtctaccagc tgacccacac ctaccggctg
    11761 gcgatcatcg ccctgatcgt cttcttcgtc ctcggcggca tcctgctctc gcgggtcgat
    11821 gtccgtgaag gcatccgcca ggccggcaac gagcaacctg tgctgatcta ggacacgcga
    11881 agccggtcca ggacgcgcga agccggccca tcttctccgg agaaggtggg ccggccacgt
    11941 cgtgcctcga ggtgcgccgg tgcatcgtca gccgctgcgc cgggacggcg cgagcggctc
    12001 aggccgagcg cttgctcggc ttctcgccgc gggactgacg cagcagcgtc agccgctcct
    12061 cgaggagttc ctcgagttcg gggatggagc ggcgctcgag cagcatgtcc cagtgggtgc
    12121 gggccggctt gaccggcttg cggtcgggcg cggcgccgtc gagaagcagc gcctcggcgc
    12181 cgcaacggca ctcccaggtc gcggggatct cggcttcgat ggagaacggc aactcgatac
    12241 ggtggtcctg cgggcagacg tagacgacgg tttggcgctc gctggggacg acgttctcgg
    12301 acgtctccat gctgaaggac accatgcggc taccccgcaa tgtgcggtcg gccatgggtt
    12361 gaactcccta aataactcgg tgctggtctc gcggtgggtg caggccgcga gggtctatgg
    12421 atatgaacgc gcgagcgcgg cggcattgtt ccgagacgga tatggtgcgc cgattcgcgc
    12481 ccggcggacg gggagcgctc gcgccggggg tgtcttgacc ctccgcggtg agggccagac
    12541 attacagtct accggtcgac gacacgtcct tcgaccgtgt ccgggctcgc aggcctgtga
    12601 cctgggcgac ttccgggaaa cttggctccg gcggcggctg cagcgccccc gagaaggcgg
    12661 gcgcgcaccg cctgttcgag caggcgccga gtgatcgggc gggtgacggg caggatgaag
    12721 aagaacccga cgatgtccgt gaggaagccg ggcgtcagca gcagggtccc gccgatcagg
    12781 accagcgccg cgtcggccag ctgacgtgac ggcatggcgc cggtggtcag ggcggtgttg
    12841 agggccttcc acgtccgggc gccttcgcgg cggaccaacc aggccccgag gaaggactcg
    12901 aagagcagga gcgcgacggt cggccagccg ccgatcacct tgcccaccgc gatgatggcg
    12961 gcgatctcga ggaccggcac gatcagcagt gcgaggagga gccacagccg ccacggccgg
    13021 cggcgcgacc ccgcggcccc cgccgcatac ggtgtggacc cgctcaccct aggcatccgc
    13081 ggactccgtc gtgactacaa cttctcccat tgcgcttccc gcttcacgag cttcttgacc
    13141 tggccggttc ggtgttcgat tccccaacga gtgacgcgcg ccatggattc cttgacgatg
    13201 tcgttgttca tcttcgaggc cccgatctca cgctcgacga actcgatcgg cacctcaacg
    13261 atcttcaggc cggcccgcac ggcgcgcacc gtcaggtcgg tctggaagca atagccctgc
    13321 gaggcgacct gctccaggcc gatggtgcgc agcgcgctcg cgcggtagac ccggaatccg
    13381 gcggtggcat cgcgcacacc catcccgagg atcatgcgga tgtagaggtt gccgcccatc
    13441 gacaaggctt tgcgggaggc cggccagttc accacggagc cgccgcggac ataccgcgac
    13501 ccgatgacca cgtcggcatc ggcgatggcg gcaatcatcg ccgggaggta tttcggctgg
    13561 tgagagccgt cggcatccat ctccatgatg gcgtcgtagc cacgctcgag cccccagtcg
    13621 aagccggcga gataggccat gccgagcccc tccttgccgg cgcggtgcaa cacgtgcaca
    13681 tgctcgtcgg ccgcggcgag ctcgtcggcg agggcgccgg tgccgtccgg cgagttgtcg
    13741 tccaggacga ggatgtccgc ctcgggggcg tgctcgcgca cgccggccac gacgagcggc
    13801 aggttctccc gctcgttgta ggtcgggata cagatcagga cccggaggac gggttcgcgg
    13861 gtgccagtgg cgtcagacac gtgttgcttc tttcgcgctg ctcgagcggg tggttgacgg
    13921 gccattatcg cggcgccggg ggccgcgacc caatccccac gccagtgaca gcccagctag
    13981 ggcggtgtat tccggtatgc ggccgagccg gtccgcgatg gtcgtcccct ggccgagggg
    14041 caggtcggcg acggcacggt atgcggtgta caacgacgac ttgtcggcat agctcccgtc
    14101 ggggcggatg aagccgctca cccccaccgt ggagacgtgg gcgacgggcc ggttgtgttc
    14161 gatggcgcgc agccgggaga tcgcgaactg ttgctcggac tcggcggtgt agccgaaggt
    14221 ggcgttgttg gtctggacca ccaacacgct gggcacctgc ggactgtgcg tgactgcctc
    14281 ccggatcaga ccgtcatagg ccacctcgaa gcagatcgtc ggcagcgcgg cgaacggggc
    14341 gccggagcgg gtctgcaacg cgaagtaacc cacctgcgtc cctgtggcca tgccgtcgcg
    14401 gacgaggtcg acattggagt tgaagcggcg gaagaagtcc ttgtatggaa cgtattcggc
    14461 gaacggcacg ggatgctgtt tgacgtagcg ctccggatcg aggacgccgg ggcgatagag
    14521 cagcgaggcg ttggagacca agggcgccgg ctcctcgagg atggcgccga gcaggagcgg
    14581 ggctttgacg gcgttgcggg cgtcggtgat gacggcggcg gcgtcggtgt tgcgcagcgg
    14641 gtcgatgtcg gcggcgttct cgggccagat caccaggtcg agagcgccgg cggtggcggc
    14701 cagggcctcg gttccgcgga cgtggttgtc gaggaccgcc cggcgttcgg cgttgaagtc
    14761 cagtccggcc ttggggacat tgccctgcac gatcgccact cgtgcggtgg tgctcgacgt
    14821 cccagcgagc ggtatggcga ggggcagcgt cgccagcgcg acggccgcga cggcgggcac
    14881 caggacgcgg cgacttgacg tcaggacgcg gcgacgagac gtcagggcgc ggctgcgagt
    14941 ggccagggcg gcgcgagcca cggcatacag cagcaccccg agcgtggcga cagcggccgt
    15001 gagcagcggc gcaccaccaa ggctcatcag gttgcgcagc ggcgagtcgg cctggctgaa
    15061 ggccagtcgc gcccaaggga atccgccgaa gggggtggtg ctgcgggccc actcccccac
    15121 gacccagccg agaggcacgg tcgcggaggc cgcccacccg agccggcgct cgaccaaccg
    15181 tcgctggatc gggccgatga cggcgaccat cgccgcgaca tacagcgcct ccagcgtcgc
    15241 cagagcgatc cacggcagct tgccgacgaa cacccccgac cacgagagcg tcgggacgaa
    15301 ataggccagg cccgccacga gaccgacgag gaatccggtg cgccagcgtg cgacccagcc
    15361 ggccgcggcg atcaagccgc agccgagcca cgcggcatac cactgattgt ggtcggggaa
    15421 ggcgagcgcg atcgcgccgc cgccggcgag ggcgaggagc aggcgcgccg gcaggatcgg
    15481 ggcaggcacg tcgcgactca cggagcaacg gtacgtcctg cggggtcgca gctttgccat
    15541 gctgaccgcc aacgacgtcg cccggtgcga gcggtggttc gacgagaacg ggctgccgca
    15601 cttcgtcgag cagcaacctg atgctgatgc tgttcatcac ccaggcggtg cagctgttgt
    15661 tggtctcgct gctggtgttc ggcttcttcg tgctcttcgg ctcgctcgtc atccggccgt
    15721 cggtgatcga ggcgtggacg gggtcggctc cgcactacac cgggccactg cacctgatca
    15781 gtgacgcact gttctcggtt gccgtctttc tctccgggtt cgccggtctg tatttcacgg
    15841 tgcaggcggt catcgaccag aactaccgca aggagttctt ccgccgcatc gaggacgatc
    15901 tgcagcaggc gatcggggtg cgcaaggtct atacgacgct gcggacccat ctggcgcctg
    15961 gggcggggac ctcccggttc taggctggag gcatgtccga cgcggtcgac gtcctcatgg
    16021 tcgacgacga gtccgcgctg gtcgccagca cgctcgaata tctgcaggcg ttcgggctca
    16081 cgacggcagg ggtgggagat gccgaggcgg cgctcgcgtg gctgacgcgg cataccgtcc
    16141 gggtgctgct cctcgacgtc aacctgccgg ggatgagcgg cttcgatctg tgccgccggc
    16201 tgcgctccac ctcccagacg ccgattctct tcctcagtgc ccgcgcggcc gacgacgacg
    16261 agatcctcgc cctgagcctc ggcggcgacg actatctgcg caagccgtat tcgctcgggg
    16321 tgctgctggc caagatccgc cggatcctgc aacgcagctc ggccgccacc gacgtgcccg
    16381 ggtatgacga tgggcacctg cgcttcgatc cggccgccga ccgcttctgg gtcgacgggg
    16441 tcgatctcgt gctgtcggcc atggaacacc ggctgctgcg ctatctggtg ctcaaccggg
    16501 agcggatcgt gcccaaggac gagctgttcg agcaggtgtg gggcgacgcg atcacgggcg
    16561 acggcacgtt gagtgtgcac atccggcggc tgcgcaccca catcgagccg gatccggcgc
    16621 agccgcgcta tatccagacc ctgtgggggc gaggctattc gttccgggat ctgtcgtgag
    16681 gcgctggctg gcgctgctgc tgttgtgggc ggcgctcggg gcggccggtc tggccgtcgc
    16741 tcgggcgagc gtgccggccg ctccgccggc ggatgttgcc cgcgcgaacg acctcgcgca
    16801 gatcgcggcc gagcactggc cgtcgttggc acgcaacgac ttcccgtcgg atgcaccgcc
    16861 cttcgcggtg gtggccgcgg acggccgcgt ggtgctcgac gcgggtgcgg gggtgcgtga
    16921 cgacgtctcc gcgtatgccg ctcgagcgct gggtctcgat gtggtgaccg atggcgggag
    16981 cgtcgcgcgg atctatgtcg tcgatccgta tgccgacctg ctggccgacc gcgcccggcg
    17041 ggcccgcgac atcgcgctct ggtcactggc ggcggccggc ctcgcggcca ccgggtggct
    17101 gtggtggctc gaccgccgga tcttgcggcc cttccggcag cttcggggtc tcgccggcca
    17161 ggtggcgcgg ggccgtctgg atgcgccgct cgccgtgcac cgtggggacg ccttcggtgc
    17221 cttcagtgag agtttcgaca tcctgcgccg tgagctggcg cgctcgcgag agcgggaggc
    17281 gcaagcccag caggcccgca aaacccttgt ctcccaattg agtcacgaca tccggacccc
    17341 gctggcgacg atccacgcga ccagtgaggt aatgcagctg tcggacccgg atccgcggtt
    17401 gcaggtgatc atggacaagg ccggtcagat cgatgccttg acgcgagatc tgttggcggc
    17461 caacgcaacc gacgccgagg acctcggtgt gacgctgacg gccatcggca ctccggagct
    17521 gcgggagctc atcgcggcag ccgacgccgc cggttgggtg agcgaatgcc acctgcccga
    17581 cgtgctgatc gtcgccgacc gggcgcggct ggcgcaggtg ctcgacaatg tcttcggcaa
    17641 tgcgcgcaag tacgccgctc ccccggtccg cgtcgccggg gccctggagg acgatttgct
    17701 gacggtccag atcacggatc acggaccagg agtccccgag ggcgagctgg cgctggtgac
    17761 ccagcggcac tatcgcgcgg ccaacgccgc gggagtccag ggacagggtc tcggcctgca
    17821 cacggcatac acgctgatgg acgccatggg tggcgcgctg gtcgtcgcca atgcggccga
    17881 cgggttctcg gtggcgctca cgctcgcctg cgcggagcgg cagcacgacc cgcggcggca
    17941 cgcgttcaaa accatttaag agtctcgaaa ggactcggcg gcaccggctt tcctaccgtc
    18001 gaggtatgac gcagatccag cagcagggga cggtcctgaa ggcgtcgcag atctccaaga
    18061 cctatgcgca cgacggcctc caacaacaca tcctggtcaa cctcgacctg gacatcaccc
    18121 ggggcgagta cgtggtggtc atgggtccgt ccggtgccgg caagtccacg ctcatgcatg
    18181 tgctcagcgg gatggactcc ccgacgctgg gtgagatcga gttcgacggt gcggcgatcg
    18241 gcgcgttgaa cccgcggcgc ctgaccgact tccggcgcga ccattgcggt ttcgtcttcc
    18301 agcagattca tctgctcgac tcgctctcag tcctcgacaa tgtgctggcc gtcggtctgc
    18361 tcgggcgccg ccgccgccgc gccgaggtcg tccggcgcgc cggcgaattg ctcgaccggg
    18421 tggggatcga ggaggcggac cgcggcaagt tcccggccat gctgtccggc ggcgaagccc
    18481 aacgcgtcgc cgtcgttcgg gcgctgatca actctcccgc agtggttttc gcggacgagc
    18541 cgaccgggca gctcaacagc gagtcgtcca gcgccgtgct cgacctcctc ggggaggtga
    18601 gcgcgggcgg acaaaccgtc ctcatggtca cccacgacct gcgttcggcg ctgcgtggag
    18661 accgggtgct ctatctgcgc gacggagcga tccgtggcga gtgtgtcctc ggccggcgcc
    18721 gggacgcgga gcacgatcac gatcgtctct cccggctcac cgggtttctc gacgagatgg
    18781 ggtggtgacg atgtcgatcc ggccggcgtc ctccttcggg gtgctcgcgc tgcgcaatct
    18841 gcgccgcgca cgagtgcaac tggctgtcgt agcgctcttg agccttgtcg ccggcgcgct
    18901 gctcaacctc ggcgcactgt gcggtatccg ctatccccag ctgatccgcg gtgaggccga
    18961 acgtctcgac gcaccccaga ttgtgctgct gacgcccacg accgaggcgg cgcgaacgca
    19021 ggcatacttg cgatcggatg cccgtgtctc gcaggtcgat tcgatgacgg tgctctccga
    19081 gcgagcctcc ctccgcttgc ggtcgggctc caccgcggcg gacgtggtct atgtcgatct
    19141 tgatcagcag cccgccttgg ggcgcaccaa gatcctcgag cgtggtgatg ccacggtcca
    19201 ggacccggtc tatctgcccc acctttttca tgcgaccggc gactatggca tcggcgaccc
    19261 cttcacggtg acaacgccgg ccggactgac ccgcaccttc catgtcgccg gcttcttcga
    19321 aaacgtctac ctgggattca tcacgatggg tgccaccggg ctcggcctcc cgcacgacga
    19381 gtacgttgcc ctgggcaacg ccagcacgcc gcccgaccgg ctggccttcc tccaggccaa
    19441 ggttcccgat cgcaaacaga tcacgccggt cacggcggac ctgaccgcgc gactgagcgc
    19501 tccctccggc gccggccacc gcagctgggt gtggaactgg gatggaattc agatcgtgac
    19561 catgaccggc ccgatgctct acgccctcag tctggtgctc ttcgcccttg tggtgaccct
    19621 gatcgttctc atggcgatcg gattctggat ccgcggggcg atcgaccagg acctcccggc
    19681 catcgggatg ctggagacct tcggcttccg gtccggccag atcgttcgag gtctggcgct
    19741 gcccgcggcg ctcgtcgtcc ttctcggcgc cacagtcggg gtcgtcgcgt cctatgcggc
    19801 cgtgccgctg atcgccgaat cccttgcggg acagaccggt atgccgtggc accctcgacc
    19861 cgacgtcgtc tctgccgtcg tgatcgtgct catgctcacg ctggcggcgc tggtgcccgg
    19921 catgctcgcg gcgcgctcgc tccgtcgcct ggagccggtc gccgcgttgc ggaacaggat
    19981 tgcctcacgc accttccggc gcaactggct gccgctgtcc gcggcgcgcg gcaacctgca
    20041 gatgcacctg ggcctcaaac aggccctggc caatcgcacc cagagcattc tggtcgctgc
    20101 cgtctgcctc gtggtcggcc tcggctcgat cttctccgcc agcttgttca ccaacgtgct
    20161 gagcaacaag gagaacttca tgcacatgct cgtcggcgac tacgggcaag tggcggcgcg
    20221 cattgcgccg gacgccgacc cggacgctgt gctggctgaa gtgcgcgcca ttccgggggt
    20281 gcagaaagcg gtcctgaagg acttcctgca ggggtcggcc gccaacacgc aatccctcgc
    20341 tgtggtcatg gacgacttcg aggggcagac cttctcatcc gtggccgagg gacgagagcc
    20401 gcggcacgcc aacgaaatcg ccgtcggggc cgtcctggcg cgcagcaccg gcaagagtgt
    20461 gggccagacc atctccgtgc gggtcggatc gacgaccgcg gatttcctca tcgtcggaac
    20521 cctttccacg gtgcagtacg gcggcgaccg catggacttg acgacggcgg gctatcgccg
    20581 actggtgccc aatttccagc acgaagcatt ggacatcgcc gtccagccgg gcacctcgcc
    20641 gacggccgtg atcgacgcca tgtcacgacg cctggacggc cggctgagca gcgtgacgaa
    20701 tcagcagcag gacctcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc
    20821 tgcagttgat gggccagctc atcgcggcat atgtgccgct cgtcaccgca ggcacagccg
    20881 cgggatgcct cgctggcctg gtcgtcgtcc ctcgactgct cggctccttc ctggccaccg
    20941 tcggcgtcgc ccgggcatcg ctcgacacgt ccgcggcgat cgtcggaagc atcggcggcg
    21001 ggctggtggc gctggccgcg atgatggtcc tcctgggtgc cggcccgttg ttgcgccgcg
    21061 aaccggtcac gcttctcgcc cagcgatccg ggtgagaggc gcctcaacgt ggcgagtccg
    21121 cgtcagggga cgacgacgac gccgcgcgcg gtggtggcga cgatcggtgg gtcgagcagg
    21181 tcggcggcgg actcggaacg ctccgggttg ccgcgcgcga tgacccggac ctcgaactcc
    21241 atctcccggc tgcgggtgcc cgcccgcacg agccgggcga tgatctcgat gcaatcgccg
    21301 gctcgcacag gggctttgaa ctgcacatcg ctgtaggagg cgaacaggcc ctcgtcgccg
    21361 tcggtgcgga tgcacatctc ggtcgcgaca tcaccgaagg cggcgagcga ataggcgccg
    21421 tcgacgaggt tgccggcgta gtgggcgtga ctgtagggga cataccggct gtgccggact
    21481 tccataccga tctcgggtgc gctcatgcgc tctttccctt cgggcgctcg atcatggcgt
    21541 gcacgaggta tgacgcgacc tcaccaggcg tggtgccgcg gccgaagatc cggtccacgc
    21601 cgagctcctt ggcggagccc tcctcgaagc gcggaccacc gacgatgagc aagggcacct
    21661 tgccggcggg gaacgcctca cggaaggcgg cgctcatcgc ggtcgtgttg tggatgtggg
    21721 cgtccttctg ggtgacgacc tgggagacga ggacggcgtc ggcacgcagg tcgtgggcgg
    21781 cgttgacgag ctcggggatc ggcacctgcg cgcccaggtt gtgcacggcc atctcccggt
    21841 agtactccag gcccttctcc ccagcgaatc ccttgatgtt gaggatggcg tcgatgccga
    21901 ccgtgtgggc gtcggtgccg atgcaggcgc cgacgacgac gagcttgcgg cgcaagcgtt
    21961 tccggatcag ggcgttggtg tcacgcgcgg agaggacgtc gaagtcgcgt tcgaccacct
    22021 cgacctcgcg caggtccacc aggtgggtga cagcgccgta gacgacgaag aaggtgaagc
    22081 tgggggcgat cgacttggcg tggaccagga tcgccgggct catgcccatc ttggcggcca
    22141 gctggagggc ggcgccctcg gcccgcttgt cgtgcgggac gggcagggtg aaggagatct
    22201 gcaccatgcc gtcgccggtg gtgtcgccgt agggacgcac cagcggcgat tcctcgtgca
    22261 ggctcaactc gccggggagg gcgtcttcca tgcggcgttg cccgacgtgc tggcgggact
    22321 tgttgcgggt ctcttgagcc tcggtgcggg gtggggggtt catcggtcgc tcccctccga
    22381 aagcaggtcg gtggcggggt tgaggtagcc gggtgccttg cgcgcgacgc cgtcgagacc
    22441 cttgcccttg gtgggcgggc gtttcatggc gccgaacgtg ccgtcggcga tggcggccaa
    22501 cagtgccggg tcgccggtct cctcggcgtc gtcaacgatc tcttggagca ggtcgatcgc
    22561 ctcccggagc acctggcgcg ccctggtctg gatgaggccc tcgcggggcg gatggaagtc
    22621 ctcggtcagt ccaccggcgg cgtcgaggac gtagcgcacg ttctgcaggg ccaggtcccg
    22681 gtccgaaagc cacggtgtca caacggcttc ggtcatcatg cccacgagca ggatgccctg
    22741 cccggtcatc gcgccgacga gattgaagaa gccgtcgagc aggtagccac ggaagatatc
    22801 gccggtcatg tgcttggtcg gcggcatcca cttcagcggc gcgtcgggga agagcgtgcg
    22861 ggcgagcagc gcgtgcgcga gctcgagccg gaagctgtcg gggacggtgg ggtcgatctc
    22921 gaaggcgtgc ccgaggccga gttgccagtc ggccaggccg gcctccttgg cgaagtactc
    22981 gttgagcagc tggctgacgg tgacggtgtg cgcggcctcg acagcgtcgg cggtggtgag
    23041 gtaattgtcc tcgccggtgt tgatgatgat gccggcgcgg gcgtggatct gccgcgaaaa
    23101 gcgttggtcg acaaaggttc tgatcgggtt gatgtcgcgg aagaggatgc cgtacatcga
    23161 gtcgttgagc atcatgtcga gccgctcgag cccggcgagg gtggcgatct ccggcataca
    23221 caacccgctg gcgtagttcg tcagccgcac ataccgcccg agttccttgc tcgtctcgtc
    23281 caaggcggcc cgcatcaggc ggaagttctc ctgggtggcg taggtgccgg catacccctc
    23341 gcgggtcgca ccctccggca cgaagtcgag caggctctgc cccgtgctgc ggatgacggc
    23401 gatgatgtcg gcgccctcgc gcgccgccgc ctgcgcctgc ggaatgtcct cgtagatgtc
    23461 gcccgtggcg acgatgaggt agatccacgg cttctgcttc ggatccccgt gccgggcaat
    23521 gagtttgtcg cgggtcgccc gctgcttgtc gatggtccgg acgccgcggc tgatggccgt
    23581 gcgggccgcc ttgcccgccg cagtggagtc cttgccctcg gggagccgga aggtcaccgc
    23641 tcccccggcg gccttctgcg ccaaggtcgt caggtccggc gcgtcgcccc ggcgcagggc
    23701 atcccacacc ggaagcgcga cgccatgctc caggcccacg tcctggcgga cccggtcgac
    23761 caggtggttg acccacggca cccgctcgtg gtcggccccg ctcagccccg cgagccgcag
    23821 cgtggcccgc tccaccgaga cggtcgtatg ccgcttggcc aggttcacca cgggccgccc
    23881 cgcctggcgc gccaaagcac gcgcccgttt cacctcagcc gggtcgagtt cgaggacgct
    23941 ccgttgccgc aaaaccaccc gtccagcata tcgccggtcg taaattatcc gctacatgcg
    24001 gccaaatgcc gcatgtcttg gcgcactagt cccgctgcag tgcagtttct gcaggccgcc
    24061 ggcgtatccc tggcgccccc gcgggagtag acaagcgccg ctgacgcccc aagtggtcgt
    24121 cggtgttgcc gccgggacct gtgatgacga gggctgcgga gctcgcggct tcacgatctg
    24181 ccgaggaatg cccaatgccg ttgttggcga acgtcgccgc cagtacgtta gccgcagagt
    24241 taacgacttt ggaatcgacc ctctctacgc cgaaactcat agcagcgcgc gtgctttcgg
    24301 tcagcacccg cagcgccatg ctcgcccaac tccgtcaact gcgaagcgcg gtcggccagg
    24361 ttcaggccgc cgcccagcac aaggtcgaca ccactcgcgc ggctctggcc ggtgcacgag
    24421 cccaagcgga agcggcggcg cggcagtcga cgcagggggc caaggatgtc gcgcgcgcgg
    24481 ccaaggatca ccaacgtgcc gaagccaaga aggcggaggc caagcgcaaa gcggaggcga
    24541 cgcgaaaggc ggaagcgaaa cgcaaggccg aagcaaagaa gcaggcggtc gctgaagcca
    24601 agcgcaaggc cgaggccgaa gcggcggcca agcgcgccgc ggaggctaag aaggctcagg
    24661 tgcccgcaac acctcgatcg tccactcctg ctgcgccgcg gacacagcag ccgaccacac
    24721 ccaacgtccc ggccaatccg ggattgacat acaccggctg ccgcgcctac gggccaaacg
    24781 gcatttggct cgacgcgaag gggcggcggt tctcgccaat cccctgctga cgattcagtc
    24841 agcgaggcga cgctccacgc gctcgacctt gccgtcgagt tcgccagtat ggccggggcg
    24901 gatgtcggcc ttgaggacga gtgagacccg cggggcgccg gcgccggcgg cctcggtggc
    24961 gcgcttgacg acgtccatca cctcgtccca ctccccttcg atctcggtga acatcgagct
    25021 ggtgcgattc ggcaggccgg actcccggac gacgcggacg gcggcggcga cctgctcgga
    25081 caccgagtct cccccggtca tgggcgcaac tgagaaggcg acgatcatgg ctccactatg
    25141 ccgtggctcg ggacgcggtc acgccagatg taggtgggtc ctggccggca cgcccggcca
    25201 cggctttgcg tgtgggaagg ttgggcaatg ccgacgagcg actacgagca ggacacctcc
    25261 accggcttcg gtccgcttcg catccgcgcc cgcggccgag cggtgatgac caatccgacc
    25321 atcaaccgcg gcacggcatt cacgcagccc cagcgcaagc aactcggtct cgtcgggctc
    25381 gtcccctcca gcgtgacgac tctggagaac caggtgcggc gcgtctacga gcagtaccgt
    25441 cgcaccacca cgcgcctggg caagtacctc cacctcaacg gcgtccggga ccgcaacgag
    25501 gtcctcttct accgcctcct caccagcaat atcgaggaga tgctcccgat catctacacc
    25561 ccgacgatcg gtgaggcgat cgagcggttc agccacgaat tctcccgtcc ccgtggggtt
    25621 ttcctctcca tcgaccaccc cgagatgatg gaggagtcct tcctcaactt cggtatcgag
    25681 cccgacgagg tcgacctcgt cgtcgtcacc gactccgaag gcatcctcgg catcggcgac
    25741 cagggcatcg gcggcatcca gatcgccatc ggcaagctca acgtctatac cgccgctgcc
    25801 gggatccacc cgcgccgctg catacccgtg gttctcgacg tcggcaccga caacctcggc
    25861 ctgctgtcga gcgagatgta tctcggggag cggcacgcgc gcgtgcgcgg ggagaagtac
    25921 gaccagttcc tcgaccgctt cgtccatacg gtcaccgagc tcttccccaa cgccatgctg
    25981 cactgggagg acttcggcac cagcaacgcg caccgcctgc tgacgaagta ccgcgaggag
    26041 atctgcacct tcaacgacga catccagggc accgccgccg tcgtgctcgc cgcgatcctt
    26101 gccgctgtcg acgtcacggg cctgccgctg gacgaccacc gcgtgtgcgt cttcggcgcc
    26161 ggcagcgccg gcatcggcat cgccaacctc atccgcgaca cgatgctgcg caccggagcg
    26221 tcggagaccg aggaggaggc ctacgcccgg ttctatccca tcgggatcaa cggcctgtat
    26281 gtcgatgacc agccggagtt gctcgccttc cagcgaccgt atgcccgcac ccgagcggag
    26341 gtcgagcact gggcggtggc tgaccccgcg cacatcacgc tcgaagatgt gatccgcaat
    26401 gtgaagccga cgatcctcat cggaacctcc acgcgcggag gggctttcac gcgcgggatc
    26461 gtcgaggaga tgtcgtcgta ctgccagcga ccgatcatct tccccctgtc gaatccgacg
    26521 agccgcgccg aggcgacgcc ggcggatgtc ctgacgtgga gtgacgggcg cgcgctcgtc
    26581 gcgacgggca gtccgttcgg tccggtggac tacgacggca cccgctacac catcgcgcag
    26641 gccaacaacg ccctcatctt ccctggcctc ggcctgggtg tcgcgacctg ccgggccacg
    26701 cggatcagcg aggggatgat cgccgcagcg gccgaggcgc tcgccagcat ggtcaacgcc
    26761 taccggcaag gagcgccgct gctgccgagc atcagcgcgc tgcggcaggt gtcggccacg
    26821 gtcgcggtgg ccgtcgccaa gcaggccgag aaggaggggc tggcgcgaga gccgctgacc
    26881 gacccgatcc agcagatcta ccagcggatg tggcagccga tctacccgga gatcgaagcc
    26941 gtctgaagcc gcacagcccc aacggatttc acgctcggaa tccatttcac gcaccgaatg
    27001 actgaccaag gagttgccat gacccaactg ggcatcgact atctcgccga cgccgaccgc
    27061 tacgaggggc ggatgcccta tcggccggtg ggccgctccg ggctgcggct gcccgcggtg
    27121 agcctcgggc tgtggcacaa cttcggcgac gatgtcccga tggagcgcca gcgggcgatc
    27181 ctgcgccggg ccttcgatct cggtgtcacg catttcgacc tggcgaacaa ctacggcccg
    27241 ccctatggct ccgccgaacg caacttcggc gagcacctgg ccgcggattt ccgcccttat
    27301 cgcgacgagc tgatcatctc ctccaaggcc ggctatgaca tgtggccggg gccctacggg
    27361 cagggcgggg gcatgcgcaa atacctcatc gccagctgcg atcagtccct gcggcgcatg
    27421 ggcctggact atgtcgacat cttctacagc caccgcttcg accccgacac tcccctcgag
    27481 gagacgatgg gggcgctgga ctacatcgtg cgctcgggcc gggccctgta tgccggcatc
    27541 tcctcctaca gcggtccgcg cacctcggag gccgtacgca tcctgcggga gctcggcacc
    27601 ccgctgctga tccaccagcc gtcctattcg atgctcaacc ggtggatcga ggaagagccc
    27661 gccgaaggcg gcgagcccct cctcgatgtg ctcggcatga gcggcgtcgg ctgcatcgcg
    27721 ttcagtcccc tcgcccaggg gatgctcacg aacaagtacc tcaagggcat tcccgcggat
    27781 tcccgtgcgg cacaggacaa gagcctgagc gagtcgctgc tcaccgtgga cgccctcaaa
    27841 cacgtcgcgg ccctgaaccg catcgccaag aaacgcggcc agacgctggc gcagatggcg
    27901 atcgcgtggg tgttgcggga cccgcgcgtc acctccgccc tcatcggcgc atccagcgtc
    27961 agccaactgg aggacagcgt ggcggcggtg aagaacctcg acttctccga cgccgaactg
    28021 aagtcgatcg accggcacgc cgtcgactcc ggcatcaacc tctgggccgc ttcctcggcc
    28081 ggctagctcg tgggcttggc gacctgctgc tgctcagggt cgaccgcctc cgccnnnnnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggctg acgcgcacgg tcagagcccg
    28261 ctcgctgcag ccgaggacag ctgcggcgtc cgcatacgtc aggccgtccc acgcgatgag
    28321 caagagggcc tcgcgctcgg cggggctgca cgccgccagt gccgcgatca tcgcgtcacg
    28381 tcggcgacgg cctcgtcggg cggcagcggc gcaggcgttc gccagtattt cgaaaccgct
    28441 tgtcgccaaa gggattccgc gcgccgctgg ccgcgcgtgc ggttgtggat cgtatgtcgc
    28501 gcggtcacca ggagccacgg cagcggctcc gcggggagtt ctgcgcggcg gcgccaggcg
    28561 tgcaggaagg tctcggagac cacgtcctca cactgtcgat ggcgatgtgc cggcgggcgt
    28621 aggcatagac ccgcggcgcg tgctcccgga agagctgggc gaaggccagg tcacccaccg
    28681 tggccccctc ggtgtcgtgg tcgcatgccg ggtaatgtcc gcccggtcca cgatgtcaca
    28741 acacccgcgt cacgacggcc aggccttact cattccgaga ggcgggcctc gaacatggcg
    28801 cgtacgtcgg cgtcggcccg cagcaggtcg agggcgtatg ccgcgtggcc gggcacataa
    28861 ccgttgccga cgagcatcgt cacgtcggca gcgagtccct cggccccgag cgccgcggcg
    28921 gcgaagttgg tcgccatcga gaagaagatg acggtgccgc cttgggcggt ggacaggatg
    28981 gccggctgct cacaccccgg gacgtcgacg cagaccaccg tcaggtcggc cggcccaccg
    29041 gcggccgcga cggcctgcga gagccccagc ggagagcggg catcggcgat gacgacggca
    29101 tcggccagcc tggtcgcacg caggcgctct gcctcaacct cgttgggcac cacggcgatt
    29161 cgccgcccac cccccgacgc ggccgcggcg gcgagcgcca gcgagccgga cttgcccgcc
    29221 ccaccgagga cgagcacggt cggggcggcg ccccgggcgg catacgactc gacgacccgg
    29281 cggacgaggg cgggggcgcc gcatacgtcc atgaccatca gcgcgagctc gggcgaaagg
    29341 tcctccggca gcttcgccgc gatcgagcga ccgaagagga tcgcgtgccc gtgcgcgggg
    29401 acgcgctcgc tgcgtccgtc ccaggacgcc aggccgtcgg tgatgaccag cggcgtcaac
    29461 gacagcgaga cgagggtggc tacgcggtcg ccgaccgcga ggccgagcgt cgactccggc
    29521 ccgacctcct cgaccgtgcc gatgagcatg ccgccggagc cggtgaccgg gttctgcatc
    29581 ttgccccgcg tcgcgacgat gtcgagcacc tgcgccttga gcgcctgcgc gtcgaggact
    29641 ccgtcggcgg tgtggctctc ggcgagctgg cgataggacg cggcgtcgag attcagggtc
    29701 tcgacggcga cgcgcacctc gtccggccac agcggcgagg tggcgtcgag ccggcacgcg
    29761 gcctgcggca ggctctcccc cgcgggctcg aggacgcgat gcagaccaac gggcgacgaa
    29821 acgggacggg acacggaaag atctccttgc tatccggcat accaccgcaa ctcttgcgtc
    29881 gagacttcac gaacacagga gttcttgtct atcctgtcat gtatgacagc gcacgtcgag
    29941 cagccctacg tctataccca gcgggagttg gtcgagccgg actggacccg cttccccggc
    30001 tggcgcgatg tcacccccgc gcagtgggag gacgcgcagt ggcagcgcgt caactgcgtc
    30061 aagaacatca agcagctgcg cgacctgatg ggcgatctgc tgcaggaacg cttctatgcc
    30121 gacctcgagc gcgaccaagc cgagcgcgcg acgatgtcga tgctggtgcc gccgcagatg
    30181 atgaacacga tggtggcggc caccgccgat ccgatgccgg ccgccggcgc cgacttcacc
    30241 gccgccttct acgccgaccc ggtgcgcctc tacatgctcc cggtcttctc cgaccgccgc
    30301 accgactggc cgagccaccc ctacgccacc cgggactcgc tgcacgagca cgacatgtgg
    30361 gcggtcgagg gtctgacgca ccgctatccg accaaggtgc ttgccgagat gctcccgacg
    30421 tgcccgcaat actgcggcca ctgcacgcgc atggacctcg tcggcaactc caccccgcag
    30481 gtggccaagc tcaagctcgc cggcaagccg gtcgatcggt atgccgcgat gctcgactac
    30541 ctgcagcgca ccccccaggt gcgtgacgtg gtcgtctccg gcggcgacgt cgcgaacatg
    30601 ccgtggaaga acctcgaggc cttcctcgac cagctgctgc gcgtcgacaa cattcgcgac
    30661 atccgcctcg ccaccaaggc cctcatgggc ctgccgcagc actggctaca gcccgatgtc
    30721 gtcgagggca tcgagcgggt cgccaagacg gcccacgagc gcggcgtctc cctcgccatc
    30781 cacacgcacg tcaacaacac cgcctccgtc actccgctgg tcgccaaggc atcccgcacg
    30841 atgctcgagg tcggcgtgcg cgacgtccgc aaccagggcg tgctgatgcg tggtgtcaac
    30901 gacagcgccg accagctgct ggacctgtgc ttcgcgctcc aggacggcgc gatgatcacg
    30961 ccctactact tctacatgtg cgacatgatc cccttctccg agcactggcg cgtctcgctg
    31021 gccgaggcgc agcacctcca gcacgcgatc atgggatacc tgcccggctt tgccacgccg
    31081 cgcatcgtct gcgatgtgcc gttcgtcggc aagcggtggg tgcaccaggt cgagtcgtat
    31141 gaccgtgagc tcgggatgtc gttctggcgc aagaactatc gcacctccat cgaggccgcc
    31201 gacaccgaag cgatctcgcg cgactatgtc tactacgacc cgatctactc cctccccgag
    31261 tccgggcagc agtggtggcg ctcgcagggc gaccacggcg ccgacctcga gatcgccatg
    31321 gcccgcgccg cggcgtcgcg cgacgccgcc tcccgcgccg cggacctgct cgccgtccac
    31381 tagccctcgc gggcggtggg tcgtcggctt tgggcggctt tctagcggtt cgcagcgcga
    31441 atgttgcgat gcgtaccgca agaaagtggc actgggcgga aaagtgcgtc atggcccgcc
    31501 cgcggtgacc gggaccacgt ccagaatgcg accccatggg gtgtgttgcg gcgggcgcgc
    31561 cgataggctc acgatcgtga ccgccatcgt cgaacgtccc cgcgtccttg ccgatgtcat
    31621 ccccggaggg ctggtgcgcg atgccgcgct cgtcctcggt ggcgccggct tcatcggcct
    31681 gctggcgcag tgggccattc cgctcggctt caccccgatc ccgctctcgc tcggcacctt
    31741 cgcggtgctg ctgacgggtg ccgccctcgg cgccggccgc ggcctggcat cggtcggcat
    31801 ctatgtcctg gccgggatgg ccggtatgcc gtggttcgcc gagcaccgca gcggctgggg
    31861 cttcccgagc ttcggctacc tgatcgggtt catcctcgcc gccacgctcg tcggagcgct
    31921 cgcctcccgc ggcggcgacc ggaccgtgct gaagaccatc ggcctgatgg tcctgggcaa
    31981 cctcgtcatc tacgccgtcg gcgtgcccta tctcgccgcc aacaccggca tggatctgag
    32041 cacggcgatc gagaagggcc tgaccccctt cctcctcggt gacggcctca agatcgcggc
    32101 cgccgccggt ctcctgcccg gcgcctgggc cctggtccgc cgcgtgcgct gaggcacgtg
    32161 gccaagaagt cgacgcacac ctccgggacc ccggcaacgg tcgccttgac cgcggccggg
    32221 gttcgcttta cgcaacacac ctacccgcac gaccccagcg caccgtccta cggactggaa
    32281 gccgcggaag cactgggagt gccgcccgcc caggtcttca agacgcttct cgtcgacctc
    32341 gagggcggac ccgaccggac cgccgtcggc atcgtgccgg tcacccagca actggacctc
    32401 aaagccatgg cgaccgcctg cgccgccaag cgcgcaacga tgtgcgatcc cgttgccgca
    32461 caacgcatca ccggctacgt cgtcggcggc atctcccccg tcgggcagaa gcgcgcgctg
    32521 ccgaccctgc tcgacgagag tgctgcggcg caggccgtgg tgtatgtcag cggtggccga
    32581 aggggattcg acatcggcct tgccccggcc gaattgctgc gcgtcatcgt cggacggtat
    32641 gccgccatcg cgcgttgatc tgcgccttac tcccaggggg tggagagcac gatcgtcgtg
    32701 tgggtcgaga cgttggcggc ggcgcggatc cggctgatga gtgactcgag cgcgccgggc
    32761 gtggcgacgc ggaccttcag caggtagttc gaatccccgg cgaccgagtg gcaggcctcg
    32821 atctcggaga tcccgcgcag ccgctcgggg atgtcgtcgg gcgcggagag gtcgaacggg
    32881 gtcacggcga tgaaggcggt gagcgggtca ccgagctggt catgcgcgac acgggcgccg
    32941 tatccgagta gcaccttgcg ctcctccagc cgccgaaccc gttggtggac agcggatgtc
    33001 gacaagccga cggccttgcc caggtcggta taactcatcc ggccgtcctg gcgaagcagc
    33061 tgcacgagtc ttcgatcgac gtcttccacg ggacaagctt tactgcgcgc ggcgcgaagc
    33121 gcaatcggac ttttcggcgg gctcgtgagc aggcggcatg atgtgccgta tgaccgagcc
    33181 ccgcttgatg ctgctggact ccgcgagcct gtacttccgg gccttcttcg gcgtcccgga
    33241 cgaccggccg gatgatgccc cgggcccggc cacgaacgcc gtgcggggtt tcctcgactt
    33301 catcgccacc ttggtgcgcg agcatcaccc gacccacttg atcgcgtgct gggacaacga
    33361 ctggcgcccg cagttccggg tggacgcgat cccctcctac aagacccatc gggtggcgga
    33421 cggctcggtc gaggaggagg aagtgcccga cgccctgact ccgcaggtgc cgatcatcgt
    33481 cgatgccctt gcggcactgg gcatcacgcg cctcggcgcc gacggctacg aggccgacga
    33541 cgcgatcggc acccttgtcg cccggcacaa gggcacgatg ccgatcgacg tcgtcaccgg
    33601 cgaccgggac ctcttccaac tcgtcgacga cgctgctcac gtacgcgtgc tgtacaccgc
    33661 ccgcggcggg gttcgcaacg ccgatgtcgt cgacgaggcg ttcctgatgg agaagtacgg
    33721 cgtcgccaac ggcgagcagt atgccgatat ggcggtcctg cgcggcgaca cgagcgacgg
    33781 cctgcccggc gttgccgggg tcggcgagaa gaccgcggcg aagctcatca cggcatacgg
    33841 ctcgctgacg gccctccggg aagcgatcga cggcggcgac ccggcgatca agggggcgca
    33901 acgccgcaat ctcgaggcgg gctcggccta tctggatgtc ggtcccctcg tcgtgcgcgt
    33961 cgcccaggac gcgccggtgc ccgacgagtc gggcgagttg ccgaccgaga tcgccgatct
    34021 tgcgggtatg tcgcggctcg cctcggagta cgggctcacc tcgcccatca accgggtgat
    34081 cgacgccctc ggcctcgggt gaacgggccg gcgggctgca ctcggtgcga ctgggccacc
    34141 cgggcgggtc agcgggcgaa cgttcggcgg tagtcggtcg gtgggacccc gacgtgcggg
    34201 gcgaagtggt ggcgcagcag cgccgagctg ccaaaaccac accggcgggc gatggtctcc
    34261 acgtcgaggt cagtgctctc cagcatacga cccgacccca taggcgcaac cctcccaccg
    34321 aatgcggtat atcgcgcgaa aacgtggcac catcggtgga gcacgtccgc accacaatc
//
LOCUS       DAOPJG010000013        32581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1446_length_32581_cov_8.133217, whole genome shotgun sequence.
ACCESSION   DAOPJG010000013 DAOPJG010000000
VERSION     DAOPJG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 32581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32581
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1446_length_32581_cov_8.133217"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKU75_02650"
     CDS             <1..519
                     /locus_tag="PKU75_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HPF79805.1"
                     /translation="QAVLDMDDTVVRGMTVAQAGETLWPPPPVQVSAAPAAAPAGGAT
                     PVADKPVKKPMSQQRRFGLIVGLGLLFVLLSAWMPGAILGNFLIFMLSVVIGFYVIGN
                     VHHALHTPLMSVTNAISGIIVVGALLQVGHGHLWVSILAFLGVLVASINIFGGFTVTD
                     RMLDMFKREEPA"
     gene            519..1925
                     /gene="pntB"
                     /locus_tag="PKU75_02655"
     CDS             519..1925
                     /gene="pntB"
                     /locus_tag="PKU75_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HPF79806.1"
                     /translation="MDVSTTLVSGAYLIAGLFFILALSGLSKHETARMGNRYGIAGMV
                     IALVVTVLLAGDRTDWNGLFWILPAMVVAAVLGMSVARKVEMTQMPELIAILHSFVGL
                     AAVLVGWNSSYEEHAAIAYPTIHDVEVFVGVFIGAVTFTGSIVAYLKLSAKIKSAPKM
                     LPAHNLVNLGIIAVFVVLTIIYCLVDPENMFLRQLLLGIMTLLALFLGWHLVSSIGGG
                     DMPVVVSMLNSYSGWAAAAAGFLLNNALLIITGALVGSSGAFLSYIMCKGMNRNFLAV
                     ISGGFGVEGGTVASDTDYGEHREITAEGAAELLREAKSVVITPGYGMATAQAQHAVAE
                     ITKVLRGKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDLADTDVVL
                     VIGANDTVNPAAMDDPNSPIAGMPVLHVWEAGDVIVFKRSMATGYAGVQNPLFFKENT
                     QMLFGDAKERVEDILKAL"
     gene            complement(2077..2991)
                     /locus_tag="PKU75_02660"
     CDS             complement(2077..2991)
                     /locus_tag="PKU75_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HPF79807.1"
                     /translation="MKSLPDDIKDLAASQHGCFTTRQSLLLGVDARTLVELKRADVID
                     NPMRGLYRTTEMIDGDSVANHRQLMHGTRLLYPDAVFASVSGVIAHEVTTWGCALAKP
                     VIRRPINRGRGNQGVIVRRRARDTVMTPFGAAVPLATALVELAIDAGTQVGIVSANSA
                     LHEGKLTDADLADAIAEMTDWPRAGRAKAMGKMAEQKCESVAESRAMFHFLTHGIPVQ
                     PQVEIFDKGRLVGRADFQIVGTKVLIEVDGKVKYADGASSVLWREKLREDALRALGYR
                     VVRVTWEDIEKPVRLLAKIRQALEFDAA"
     gene            complement(3152..4645)
                     /locus_tag="PKU75_02665"
     CDS             complement(3152..4645)
                     /locus_tag="PKU75_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPF79808.1"
                     /translation="MSVMRTKSIEQTIREGDEPEYQLKKKLTALDLTVFGVGVVIGAG
                     IFTVAGRAANQVAGPAVILSFIIAALCCGLAALCYAEFASAIPVSGSAYTFSYASLGE
                     IVAWIIGWDLLLELLLGASVVAQGWSAYLGIFLDHLGITIPESIGYGSSFDLPAFLLV
                     AVLTVLVSVGIKESMRVNMALVALKVFIVLFVIFAGIGYINRDNYSPFIPPAQASESK
                     STMESPLIEWALGYTPSHFGALGIMAGASLVFFAYIGFDVVATTAEEAKNPARDLPRG
                     IIGSLIICTILYCATALVLTGMVKYTELEAAGSLAGAFEQVGRPGYATLISAGAVAGL
                     TTVVMTLIIGASRVVFAMSRDWLLPASIGKVNPKTGTPLKITVALGTLIALTAALTPV
                     GKLEYMINIGTLSAFFLVSIAVPVLRKRRPDFHRPFKVPFSPFLPWLSAAICGYLMLT
                     LPVETWLRFLIWLALGFLIYFLYSYKNSRLAGRTDPQDSGLPSMTST"
     gene            complement(4764..5696)
                     /locus_tag="PKU75_02670"
     CDS             complement(4764..5696)
                     /locus_tag="PKU75_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPF79809.1"
                     /translation="MAAGGPTGDDPLIAPAGRDLQRTLTGDEELRLNVHQHPMVLWKP
                     IAIMLGALAVFVIIISGDQLPFLVLVLVFAAAAYLAWKMYERRHNSFAATDRRVLRID
                     GVINKSVPMMRLQKITDMRLDQPLIGRLFGYGTITIESAGQDQTLRELRYAPNPRQVF
                     RRLNSTIFGEEWAGPDQPITAPPAKRAASAAARSARRAAGATRRRAEQAAERRRPSNS
                     SGPAQSPASPIGDRASQNFFGAGADPSAAPARRRRGKESRANPPVTRRAATSTHRTTA
                     APPPAAPPASARHTAKSAQRDADTDEIPTLRPPP"
     gene            complement(5712..5888)
                     /locus_tag="PKU75_02675"
     CDS             complement(5712..5888)
                     /locus_tag="PKU75_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79810.1"
                     /translation="MSMPPIKKIVLWLLVIFLLYAILTSPDSAADIVDSAWDIVRNGV
                     RNIFEFFDALLRQN"
     gene            complement(6107..6745)
                     /gene="upp"
                     /locus_tag="PKU75_02680"
     CDS             complement(6107..6745)
                     /gene="upp"
                     /locus_tag="PKU75_02680"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPF79811.1"
                     /translation="MRVVKPDHPLIAHKLTYLRDKRTDSPTFRRLAEELMTLLAYEAT
                     RDVRVEPFAIETPVAPTTGIKLSNPKPLVVPILRAGLGMLEGMVRLLPSAEVGFLGMQ
                     RDEATLAITTYANRLPSDLSGRQVYVLDPMLATGGSMSDAIHFLIERGADDITAVCLL
                     AAPEGVARLEKNLAEVSPPVTLVVGAIDEKLNANGYIVPGLGDAGDRLYGLV"
     gene            6763..7233
                     /gene="tadA"
                     /locus_tag="PKU75_02685"
     CDS             6763..7233
                     /gene="tadA"
                     /locus_tag="PKU75_02685"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPF79812.1"
                     /translation="MATDDEQYAAWLGHALTAARAALATGDVPVGAVVVDPHGQIIGT
                     GWNAREADGDPTAHAEVLALREAARHTGEWRLTGCTLVVTLEPCPMCAGALMLARIAR
                     VVFGAWDPKAGACGSVWDIPRDRRATHRCEIVGGVREAECAELLLDFFAAHREA"
     gene            7424..7513
                     /locus_tag="PKU75_02690"
     tRNA            7424..7513
                     /locus_tag="PKU75_02690"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7458..7460,aa:Ser,seq:cga)
     gene            complement(7571..7945)
                     /locus_tag="PKU75_02695"
     CDS             complement(7571..7945)
                     /locus_tag="PKU75_02695"
                     /inference="COORDINATES: protein motif:HMM:NF022158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HPF79813.1"
                     /translation="MPRAFGIVFYLIFAMVLAVFVLVAVTIAKAGVQKRREDAANAAA
                     PVLNRPARVVAKRTAVTGGEKSTRTTYYATFEFPDGQRAEFQVDGQPYGMLSEGDTGM
                     LTNQGTWYQGFARAGRIQDRPA"
     gene            8133..8774
                     /locus_tag="PKU75_02700"
     CDS             8133..8774
                     /locus_tag="PKU75_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HPF79814.1"
                     /translation="MDLAPPLDDLIERVRALRSEPGERVILGIVGAPGAGKSTLARAL
                     TVALNESAPGTAVQVPMDGFHLADVQLERLGLRRVKGSPPTFDDVGYAVLLERLRRDR
                     QRTVYAPDFEHAVEQPIAGSINVEPSVEMVITEGNYLLLPDGAWPRVRAACHEVWFVA
                     VEDAIRVPRLIARHIQVGKAPEAAQEWVMRNDEANARLVLASAAGADLTVTMQ"
     gene            complement(8838..11117)
                     /locus_tag="PKU75_02705"
     CDS             complement(8838..11117)
                     /locus_tag="PKU75_02705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF79815.1"
                     /translation="MAPQRAGGPVDRLIAQIGSDDDVAFVASVLRTRSVAGRNVLARK
                     ATGGTLDDDGLRALGAAARLGHRGEGPSPALPRGIDKAVLAAYAHVLAVQVGRQEDRD
                     AALGIFGLLAAAGHRFTGREVRTYAQLLLFDGQLDRAVAVSQDEEVKDLVRGQIATDR
                     RNPRFAPDTHSEAEWLAAMNASLIADGLAPLALLPDGPTAFDRLTTTATTPGSVRGPL
                     IAIIMSAFNPDAKLITAVRSCVEQTYADWELLIIDDASPAPMDGVLAAAEAMDPRVRV
                     IRKAVNGGTYRARNTALRQTNAEFFTCLDSDDWAHPQRLERGVAPLLADPSLMVTRSM
                     CVRADEDLVLTRPGHHSLILNAPSLMVRMHPAVARVGFFDTVRKAADTEYAVRLEAAF
                     GRRVRDLKKDVLTMARHEAGSLSFGEFLPGWRHEARNAYRQAYGPWHQAIRDGADPFL
                     DPDGPRRFVAPARWQSPVDPRLAQPTHFDVVLLDDFRDGRPGVASSVATLRACLDAGL
                     SVGVLHTEDLWEFAPKESALDPRVLDLVNGGAAERIYYEDTVDVDSVVVPSPSILQFP
                     PWVAGCFTPARVLVASSAPASTGSSPASTLPEPSAGGAAPPPAPSTQTVTDPGRFERA
                     EVERHTVELFGVAATWVAAEELHGERLAGVLAKVARRGKPLAAERDSADSSETAWLTI
                     RTRRPADGEHADALAVRYVAGPAGHTAAVDTARALLEVLEPGVPVTAELAASAARPGV
                     QLIAQNLSGQVVQVPVPPT"
     gene            complement(11137..11748)
                     /locus_tag="PKU75_02710"
     CDS             complement(11137..11748)
                     /locus_tag="PKU75_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79816.1"
                     /translation="MTNKRVLMGLAALGGLGALAFIGAIASWWWLAVAGLAAMLGVIG
                     VVALNTNLLVRAQRSDAAKLRSAVAGGPRSAQGSAHPGEHDLTGTIRLLQAQYVGRLD
                     RAQSSLERATADLEARIGTGAAHDDPYAALPHGSVVLLHRLDEKGVSAAKTALSHGLE
                     VRAVVADSADRERLEAAGIGDAVTVVTDASDAPYALTVALPES"
     gene            11825..13762
                     /locus_tag="PKU75_02715"
     CDS             11825..13762
                     /locus_tag="PKU75_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF79817.1"
                     /translation="MPHPAAELGLARSWLVSRDLTDREIATLGETALRCATPGLRDVL
                     ALRATGGGMTADEVGAHLDSHGRWPPGALDRPAAALALACQWAVGGEQIRNQPRARVA
                     FADLLATCGPGVFAQMKRSEQLAVQLPWFAGDFTEARDLMRALPLAPEAAADLETDLA
                     NPWATGVEPTPDRVRQWLALFNRRVRDAGLAPVSVAADADNPFDALTAPTGPSVDGPL
                     VTVIMPSYAPDAGLLNSIRSMAAQTYGNLEVLLVDDASGPAYGGLYDEACALDPRVRL
                     VRMARNGGSYLGRNAALAQAKGRYITTQDADDWSHPDRMAHQVALLEANPLAPASRSD
                     AIRALDDLSLQWLGYDSQRRNASSLMLRRSTLAAIGGFDTTRKGADSEMHERLERRLG
                     PTLDTMTPLAITRLRLGSLSRSDFRYQWMSPDRVLYSTSYRAYHRSLTPQTPPLNPAE
                     QVRHRAFPAPRAYQRALPGGATLPERYDVVYLLDLSLPNALDGAAGTNLLGRGLRAAM
                     IHQEAAGVGPRRRPMTFGTAIQHALDRRVDLISTVDAVPCELLIVLTPEVLELPSEDR
                     PQVRPQHLAVLATADSETQIDQLAVLEGAREVFDLPVEFLVGNLREAEEWQQVTGEPT
                     RTVRGALGELAAAKRSATETP"
     gene            13759..>14793
                     /locus_tag="PKU75_02720"
     CDS             13759..>14793
                     /locus_tag="PKU75_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79818.1"
                     /translation="MTTAPQAPAVETRAPAVANPADANPAGATGAVGAPTPRTVMVLG
                     EEDAALRHLVTRWALVHPDRDRDRLVAVHFGKTPAKLPPGVVTIAVAGGGAILDGARR
                     AGRAKRLLDALRSLPAGRSALQDVDTLVVPDAIVDDARALADGRTVQAWSAFAAHLRA
                     DHLAALLTASAAGPSQGTGTAVLVADAVDALADTGADPSGIAAAAARALSRLLRAGDT
                     EVVASVADSVRALPHATRDRVGLDAMLRIAEISMGGPADPAADAAAAAACFAAAEQRT
                     VDGDVSDVPMLVTAGLRLIFHGDLHSDGPHSPLIDDPVGQLAPWRQSAFAARLRPKPP
                     AADADPADPAQ"
     assembly_gap    14794..14893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14894..16060
                     /locus_tag="PKU75_02725"
     CDS             <14894..16060
                     /locus_tag="PKU75_02725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF79819.1"
                     /translation="DPADPAQPAARPKIVVLGGAFGAFYQPIVDLLREAGHHPTVITP
                     DRLGVAFTRHNLTDAVVGAWLRRVDPQLFGDLPLDPGVASAIAALETAISGADVVFAD
                     WCDAEAAFASWLLPPHARLVVRVHRVDAVRAWHQLVNWPRVAALMFVSDHVKSVVERQ
                     LTDPGSPSPVPMTVLRNVIDVHRYQRPKTPGAHRRIALVGWGRRVKDPIFALDVLADL
                     VAEDDTWQLHLIGRDFAAATSPVLADYIRSFRERALDPAIRDNIRWVGFTGRLEEALD
                     ECGYALSTSNVEGWPVGVTEAAASGCIPVIRDWPQVAALGGAREIYRDHLDWVVATPA
                     EAAARIRAAADPDAWAAASAAARAAAGELCSVGSTREDYLAVILGPEPLPPPAG"
     gene            complement(16020..17174)
                     /locus_tag="PKU75_02730"
     CDS             complement(16020..17174)
                     /locus_tag="PKU75_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79820.1"
                     /translation="MTSVLVSSWAFSPWRDEAATTAAARLLSDGATRLDVVRADLDDT
                     RPREQPRPEVDDLIGRSISLPGHPVAPSTWAGIESYAAAAAGAVRAWQAAGVRWDGAY
                     SGPAGLADHLVLALLRERAVVPGWTAHLTDPPNHLASGRALTATVSPGPLRTACRALL
                     DADPVSDFLGDWALAVVAARADRVVVATAALGEHLLARAGAGSRDRLADRMEVWSRPA
                     CPVTAAPGGAGESGDVRVTARVDRDRLAQLQPVLTAYGLLPEAARPRLHLLTNVPADV
                     AVLLRESGLEGLVSLAPDLGPAASAATAAAGDYALVLDRPRPAGLPYALGDLPEIGDH
                     AAYGTPVILLVPQDSPLTRLPVAHHVPDQHPSAVAALFSRLAGAAVPARG"
     gene            17249..19510
                     /locus_tag="PKU75_02735"
     CDS             17249..19510
                     /locus_tag="PKU75_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HPF79821.1"
                     /translation="MPLRVPATLPAPPRRRNPRRGALIAAGAVAVIVSPVLASAGPAA
                     ADGERTAGPVQAAALTWTKPTFVRTIGARGSAGLYAWGVAYNPVTNQTLVGDYRNFQI
                     RRFSSGGTLLGSFYRPASSRRGVPEGLAVDPRDGSVYVSDHSKDNRGYVAKFSKDGKF
                     ICEFKLTSTYQAWMAMDANGYLYVSDSHVWHNSTNPPQVRKYSLNDTTATATEVAHFG
                     SYGSGVGEIRQITGLAIDDKTGKIAVADTINKKVNVYDGSGRFQYTFAGGVFKGDLRG
                     VAINASSRVAYVVDASAGQVERFNVDTGASLGHFGSLGTGAGQFADGGRQIAIDGSGN
                     VWVADYGNTRVLKFTATGGFIGAYPNPPQNAPKGSLALVRDVAIQPSTRQIFTIEQDN
                     HRMQAFRPDGTPAGMWGRRGTTPPLGFNYPRGLGIQPGSERFWISNTTEHQMRVLNKD
                     KSLAFNLGLPSGSPANHYNEPIDVEFGNGQVYVGDDADRNVKILNAQTGQEEGRLNVA
                     GAGVAVDPATGDIFVSSWRDRRIYHYTKNGGQGSPAVIGSAGTGAGQFQKPWDIDIVG
                     GIIYVTDATRNVLVAYERDGTFLGELGSAGTRPGQFNNPSGLTHDAAGKIYVADAGND
                     RLQVFDTKGWVKSDANPPSVSIAAPGAGATVGFPVTITGSVTDNLRNGTVEVAVQDRK
                     TKLWWDGRIATWSATKVYINAGIKGGDIRNQQWWFPLIGAQARAGYAVSVRGVDGAGN
                     IGSVVTRSFNTGS"
     gene            complement(19532..20044)
                     /locus_tag="PKU75_02740"
     CDS             complement(19532..20044)
                     /locus_tag="PKU75_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPF79822.1"
                     /translation="MAAQLTAGTPRRVRQIRAAVVLLLALAFLGGAFYKAYSYIQEDT
                     SPESAKASDCMTGTAQKPIAKVSDAKVNVYNATSTPGLAAKVAGQLRERGYSVVEIDN
                     DPLKKKVKGTGEIRYGTPGVPFARAVRLALNDAKFVNDKRTDSTIDLVIGDKFTELSP
                     LPTCSSATAG"
     gene            20288..21946
                     /locus_tag="PKU75_02745"
     CDS             20288..21946
                     /locus_tag="PKU75_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPF79823.1"
                     /translation="MASEIRDGYAFEGPAIQFGAAVVDGQVYADAPVRIPLAVMNRHG
                     LVAGATGTGKTKTLQLMAEQLVEQGVSVFLADIKGDLSGMASPGQPNDKITARAQDVG
                     MAWTPTAYPVEFFSLGGLGKGIPIRASVSTFGPVLLSKVLGLNATQESSLGLVFHYAD
                     TKGLWLDTIEDLRAVIQFLTSDAGKADLKELGGISSATAGVILRELINFSAQGADVFF
                     GLPEFDTNDLLRVASDGRGVLSMLELPAVQDRPDLFSTFLMWLLADLFHDLPEEGDLD
                     KPKLVFFFDEAHLLFNEASKDFLDAIEQTVRLIRSKGVGIFFVTQSPKDVPADVLAQL
                     GNRVQHALRAFTPDDQKALRAAVKTYPHTQYDLEELLTSLGIGEAVITVLSERGAPTP
                     VAWTRMRAPQSLMDPSPTELIDAAVNASPLTPKYLTATERDSASEMLGKKVAELDAAA
                     AAAAQEAENAKVREAAARQAEKDRLAAEKQAEKDREAAAKAAAKEAAAAEKAARADER
                     RREEDPGVVASVVKSGAFKSMLRSAGTVIGREITRSIFGTARRR"
     gene            22310..24475
                     /locus_tag="PKU75_02750"
     CDS             22310..24475
                     /locus_tag="PKU75_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79824.1"
                     /translation="MSRRSAPARSVLALLLTALTAFAVAAGAGPATGSASGSVSGSFS
                     GEARAGSVTSYVAERIIGAPSFPGVAAWGLAYNPVSDELIVGDYVSRQVRRYTRGGIY
                     LGDLRNAGKTIGGVASALGVDPRDGAIYVAVTGEGRSSLDVRKYDKEGNFLFDLDMPG
                     AATWLTVDHDGNLWVPEGFSGSVVRKFTVDDATKSAQLQLLIDKAGPGTTLKVLTGAA
                     ADAAGNIYVADVGNKVIHSWTSTGEWRFDIGPDPIRGDLRGVAIDDELGRIYLSDAVY
                     GGIRYWDMDGNYQGVISGLGLQDGEFTDGARQIAVTPDHHVYGADYGSFRVQEFLPDG
                     TFVKVFPDPVMRPDPAGISQARGLDVDPVTGDVVTVDAFGQRVNRYSAQGELLDQYGR
                     RGSSPPEGMNYPKAVAVDPATRDLWVSSFEGPPGVVGYTFDFSSVVGRPDIPRWVGDL
                     EFFGGKLYALERRPGAVRVIDPKNSKVERTWLTSVGLLHGLAVDPNNGNMWITHDSKP
                     VMYVVTPAGRIIRTITLPGVGWGVAIKGDEVVVADTTTGYLDVVSRTTYAVKGKIGKG
                     NGFGFGQLKTPSGLQFGPDGKLYVMEQVSSRVTVFAPGPAPAAETVKPVVKIDAGPMV
                     TGGQIVLTGKATDASGIAFVEARVKDLATGRFYDGKAATYLATSTWSPGVVWGDLKAA
                     SWRYTVPATLPSRSYAVTIRATDRRGNISLLLNRTVAVP"
     gene            complement(24548..26443)
                     /locus_tag="PKU75_02755"
     CDS             complement(24548..26443)
                     /locus_tag="PKU75_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HPF79825.1"
                     /translation="MSNGAYAAAYEQSISDPSAFWGKAAESIEWITPPEKVLDDSNPP
                     FYRWFAGGTLNTCFNALDRHVRDGRGGQIALFYDSPVTGYRSEYTYGELLEQVSRFAG
                     GLRNLGIEKGDRVVIYMPMVPEAVIAMLACARLGAIHSVVFGGFAPAELSARIEDAAP
                     KVVVSASCGIEPNRTIPYKPMLDAGIERSTHKPERVVMLQRPQAEAEMTARDIDWNEL
                     MAGADPVDCVEVAATDPLYILYTSGTTGRPKGIVRDNGGHAVALRWSMENVYDVKPGE
                     VWFTASDVGWVVGHSYIVYAPLLTGATTILYEGKPVGTPDAGAFWRIIAEYGAVAMFT
                     APTAYRAIKRDDPKAALLEGHDISHLRNVFLAGERLDPDTYHWANDVLHCEVVDNWWQ
                     TETGWPIAANLRGLDPMPIKPGSPSVRVPGYDVQVLDEYGQDAPRGQDGAIAIKLPLP
                     PGTLPTLWQDDERYVTSYLSAYPGYYLTGDGGMIDEDGYVFVMGRTDDVLNVAGHRLS
                     TGSIEAALAGHSAVAECAVIGVHDDFKGQIPRGLVVLKGGYDPDLEGEKIKKELIQRV
                     RDEVGAVASLKQVDIVAALPKTRSGKILRKTMREIADGKVPSIPGTIEDVGVLDALTD
                     TLKATQH"
     gene            27005..28201
                     /gene="lhgO"
                     /locus_tag="PKU75_02760"
     CDS             27005..28201
                     /gene="lhgO"
                     /locus_tag="PKU75_02760"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HPF79826.1"
                     /translation="MAEATVGVVGGGIVGLAVARALTRRRPGIRVVVYEKEDRLAAHQ
                     TGHNSGVVHAGIYYAPGSAKARLCTRGRGLLEDYLAEHDLPYRRCGKLVVALDNEELG
                     RFDELARRAGENGVPGLRRIDAAEIREIEPYAVGRAALHSPRTGITDYVAVAEALAAE
                     VRAAGGAVHLATPVRAIRARASGVEVAVPGARHGLDGLIVCAGLQSDRVARLAGGAPD
                     PHIVPFRGEYLTVATEKQERVRGMIYPVPDPRYPFLGVHFTRRVSGELEVGPNAVLAL
                     AREGYGWTTASLRDLGELAAYRGMWGLAKAHWRTGFEEMRGSLSLAAYMRAASRYVPT
                     ITRADVCRGGAGVRAQAVGADGTLVDDFVVEQEDAITLVRNAPSPAATSSLAIAEYVV
                     DGLPGR"
     gene            complement(28254..28343)
                     /locus_tag="PKU75_02765"
     tRNA            complement(28254..28343)
                     /locus_tag="PKU75_02765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28307..28309),aa:Ser,seq:gga)
     gene            complement(28428..28524)
                     /gene="ffs"
                     /locus_tag="PKU75_02770"
     ncRNA           complement(28428..28524)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKU75_02770"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(28579..29601)
                     /locus_tag="PKU75_02775"
     CDS             complement(28579..29601)
                     /locus_tag="PKU75_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HPF79827.1"
                     /translation="MNEVSGRHSAVAAFWDWWGQRGRGLAESVIGGGPKDDLVAEMSA
                     HIAAIGAGLQWEFAPGPAGGHVLVVTAAGDPELRAPARAWLRAAVPAPDWSYADLRQP
                     LPDAADTELEFAGRRLRLGDFVVAAHRGNTAIDVAVQHPVFMDIGEEEAAQLTYLALD
                     SFLGEEMVETWIGEVSWPGEPPLDAFPLQHLRTVVADFAAGFRTAEGEPQWVVLQGTG
                     PSGSPVLALAQVPLRQITFPLFDTHVAVTVPYADRTPDGLPGPGSLEALRGLEDRLSE
                     VLGADGRVVAHQSSDGVRLLHAYVDGTSEAADRVAATAAEWDQGDAQTVVTPDPGWAL
                     VNHLAG"
     gene            complement(29598..31619)
                     /locus_tag="PKU75_02780"
     CDS             complement(29598..31619)
                     /locus_tag="PKU75_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79828.1"
                     /translation="MTTTSSSSRVEPATTSGAIDRRLVLSAALMLFVELALIRWLGAN
                     VVHLSYFSNFVLLGSFLGIGCGFLISNKKWSIWPVSLPLLAALVVAVREFPVTIERQG
                     SDVIYFTALHVSGPPAWQALPVVFVLVAIILAGPAELVGRCFGQLAPLTAYRYDLIGS
                     LCGITAFSVLAFLRAPSIVWGVIAGLACLALARGLWQRILNLLAAGVLVWMLLAETLT
                     AGVSWSPYYKIQTENTSAPSSGFLLINANGVPHQLMAPAKWKVEQGERIYATPYLRLP
                     DNPLGKVLIVGAGSGSDVAIALSRGAQEVDAVDIDPRIMELGAERNVDRPYQDPRVTR
                     HVNDGRAFLENTDKKYDLILFALPDSLTLVSGASQIRLESFLFTEEAMTSVREHLTDQ
                     GAFAMYNYYRESWLIDRLAGSAKAAFGHDPCLDTFRGAQAVVAVAVDEANQSCEGAWQ
                     PAGEVIAPATDNAPFLYFKGGTFPRLYLITLGGILLASLIAIRALGGPFRGMRPYADL
                     FFMGAAFMLLETKNIASFALLFGTTWFVNALVFAGVLLTVLCAVETTRRFRTPPLKVV
                     YALIAAALALAWVVQPGRLLSLPVVPRLILATALAFLPIFLANVAFAKRFAESSESQS
                     AFAFNLLGALVGGCLEYAALVTGYNNLLLVTGALYLLAFLLVPKMARSI"
     gene            complement(31750..32529)
                     /locus_tag="PKU75_02785"
     CDS             complement(31750..32529)
                     /locus_tag="PKU75_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HPF79829.1"
                     /translation="MRIDVWSDIVCPFCHVGRRHLDLALAYFEHRDDVDVIWHSYELD
                     RNAPAVGTGSNVDRIAQKYGVTREQMVAQHEQMAAGAAEVGLDFQWDRVVPSNSYDAH
                     RLIHAAREAGVEGPVTERIMRGWYTEGAAIGDADTLRRLAAEGGLDAETIERVLSSDD
                     HGIDVRTDEAVAGQIGITAVPTFVLDQKYAVTGAQPPQVLLEAIRHAWADRANRPEPV
                     AAGGCGGGCCGGADGDGCGDEAAGAAGAACGTGGCGTCAGA"
BASE COUNT         4963 a        11467 c        11272 g         4779 t
ORIGIN      
        1 caggccgtcc tcgacatgga cgacaccgtg gtgcgcggta tgaccgtggc gcaggccggc
       61 gagaccctgt ggccgccgcc gcccgtccag gtctcggcgg cgcccgcggc cgccccggcc
      121 ggaggcgcca ctccggtcgc ggacaagccc gtcaagaagc cgatgagcca gcaacgccgg
      181 ttcgggctga tcgtcgggtt gggcctgctg ttcgtgctgt tgtcggcgtg gatgccaggg
      241 gcgatcctcg gcaacttcct catcttcatg ttgtccgtcg tgatcgggtt ctacgtcatc
      301 ggcaatgtgc accacgcgct gcataccccg ttgatgtcgg tcaccaacgc gatcagcggc
      361 atcatcgtcg tcggcgctct gctgcaggtc ggccacggac acctgtgggt ctcgatcctc
      421 gccttcctcg gcgtgctcgt cgccagtatc aacatcttcg gtggcttcac cgtcaccgac
      481 cgaatgctcg acatgttcaa gcgggaggag ccggcctgat ggacgtctcg acgaccctcg
      541 tgagcggcgc ctatctgatc gccggtctct tcttcatcct cgcgctgtcc gggctgtcca
      601 agcacgagac cgcccgcatg ggcaaccgct acggcatcgc cggcatggtg atcgccctcg
      661 tcgtgacggt gctgctcgcc ggcgaccgca ccgactggaa cggactcttc tggatcctgc
      721 cggcgatggt cgtcgcggcc gtgctgggca tgtccgtcgc ccgcaaggtc gagatgaccc
      781 agatgccgga gctgatcgcc atcctgcaca gcttcgtcgg tctggccgcg gtgctcgtgg
      841 gctggaactc ctcatacgag gagcacgccg cgatcgccta tccgacgatt cacgacgtcg
      901 aggtcttcgt cggcgtcttc atcggtgcgg tgaccttcac cggctcgatc gtggcctacc
      961 tgaaactgtc ggcgaagatc aagagcgccc cgaagatgtt gccggcgcac aatctggtca
     1021 atctcggcat catcgctgtc ttcgtcgtcc tgacgatcat ctactgcctc gtcgacccgg
     1081 agaacatgtt cctgcgccag ctgctcctgg gcatcatgac gctgctcgcg ctcttcctcg
     1141 gctggcacct ggtctcctcg atcggcggcg gcgatatgcc ggtcgtggtc tcgatgctca
     1201 acagctattc cggatgggct gccgcggctg ccggcttcct gctcaacaac gcgctgctca
     1261 tcatcaccgg cgcgctcgtc ggctcctccg gtgccttcct gtcctacatc atgtgcaagg
     1321 gcatgaaccg caacttcctt gcggtcatct ccggcggctt cggcgtcgag ggcggcacgg
     1381 tggcctccga caccgactac ggcgagcacc gtgagatcac tgccgagggc gccgccgaac
     1441 tgctgcgcga ggccaagtcc gtcgtcatca cgcccggata cggcatggcg accgcccagg
     1501 cgcagcacgc cgtcgccgag atcaccaagg tgttgcgggg caagggcgtc aacgtgcgct
     1561 tcggcatcca cccggtcgcc gggcgcctgc cgggccacat gaacgtgctg ctcgccgagg
     1621 ccaaggtgcc ctacgacatc gtcctggaga tggacgagat caacgacgac ctggccgaca
     1681 ccgatgtcgt gctcgtcatc ggcgccaatg acacggtcaa cccggccgcg atggacgacc
     1741 cgaactcccc gatcgccggt atgccggtgc tgcacgtctg ggaggccggc gacgtgatcg
     1801 tcttcaagcg ttcgatggcc accggctacg ccggcgtcca gaacccgctc ttcttcaagg
     1861 agaacaccca gatgctcttc ggcgacgcca aggagcgcgt cgaggacatc ctcaaggccc
     1921 tctaaccccg ccccccgccg accgccccgg tcgatagggc gccggcggtc ggaacttttc
     1981 cgcccagtgc cacttcatag cagtgcgcct cgcaacagtt gcgaggcgca ctgctatgaa
     2041 gccacccaga cgatcggacg atccacccgg acgacctcag gctgcgtcga attccaaggc
     2101 ttggcggatc tttgcgagca gtcgcaccgg cttctcgatg tcctcccagg tcacccgcac
     2161 cacgcggtag cccagggctc gcaacgcatc ctcccggagc ttttcgcgcc acaggacgct
     2221 cgacgccccg tccgcatact tcactttgcc gtcaacctcg atgaggacct ttgtgcccac
     2281 aatctggaag tcggctcgac ccaccaaacg acccttgtcg aagatctcca cctgcggttg
     2341 cacaggtatg ccgtgggtga ggaagtggaa catggcccgc gactcggcca ccgactcgca
     2401 tttctgctcg gccatcttgc ccatcgcctt ggctcgtcca gcacgtggcc agtcggtcat
     2461 ctccgcgatc gcatcggcga ggtcggcgtc cgtcaacttg ccttcgtgca gcgcgctgtt
     2521 ggcgctcacg atgcccacct gggttccggc gtcgatcgcc agttccacca aggccgtggc
     2581 gagaggcacg gccgccccga aaggcgtcat caccgtgtca cgggcgcgcc ggcgtacgat
     2641 cacgccctga ttgccccgcc cgcggttgat cggccgcctg atgacaggct tcgccagggc
     2701 gcagccccac gtggtgacct cgtgcgcgat gaccccgctc acgctggcga agaccgcatc
     2761 gggatagagc agcctcgtcc cgtgcatcaa ctggcgatgg tttgcgactg agtcaccatc
     2821 gatcatttcg gtcgttcgat agagaccacg catcgggttg tcgatgacgt ccgccctctt
     2881 gagttcgacc agcgtgcgcg catccacgcc cagaagcagc gattgccgcg tcgtgaagca
     2941 cccgtgctga gaggcggcca agtccttgat gtcgtccgga agagatttca tggtccggat
     3001 cattcgcgcc cgcaggtgtc gtggccagac ccgagaagac ccttgtggac aaccttgggt
     3061 gggttcatag cggtacgcct cgcgaatgtt gcgaggcgca ccggtatgaa gtggcactgg
     3121 gcggaaaagt gcgggtcgcc gcccagcccc gctacgtgga ggtcatggag gggaggccgg
     3181 agtcctgcgg gtcggtgcgg ccggcgaggc gggagttctt gtagctgtag aggaagtaga
     3241 tcaagaagcc gagggccagc cagatcagga agcgtagcca ggtctccacc ggcagggtga
     3301 gcatcaggta gccgcagatg gccgcggaca gccacggcag gaaggggctg aagggcactt
     3361 tgaaggggcg gtggaagtcg ggacgccgct tgcgcaggac cggcacggcg atggagacca
     3421 ggaagaacgc cgacaaggtc ccgatgttga tcatgtactc cagcttgccg acgggcgtca
     3481 gtgccgcggt gagggcgatc aacgtcccga gcgcgaccgt gatcttcagc ggggtgcccg
     3541 tcttgggatt gaccttgccg atggaggccg ggagcagcca gtcccggctc atcgcgaaca
     3601 ccacacggct cgcgccgatg atcagcgtca tgaccacggt ggtcagaccg gccaccgcgc
     3661 cggccgagat caatgtggca tagccgggcc tgccgacctg ctcgaaggcg ccggcgagtg
     3721 agccggccgc ctccaactcg gtgtacttga ccatgccggt cagcaccagc gccgtggcgc
     3781 aatacaggat cgtgcagatg atgagcgagc cgatgatgcc gcgcggcagg tcgcgggcgg
     3841 ggttcttggc ctcttcggcg gtggtcgcga cgacgtcgaa accgatgtag gcgaagaaga
     3901 ccagcgacgc accggccatg atgcccaggg ccccgaagtg cgatggggtg tagccgagcg
     3961 cccactcgat caacggcgac tccatcgtcg acttgctctc gctcgcctgc gccggcggga
     4021 tgaacgggga gtagttgtcc cggttgatgt agccgatacc ggcgaagatg acgaacagga
     4081 cgatgaagac cttcagggcc accagagcca tgttgacccg catcgactcc ttgatgccga
     4141 cgctgaccag gaccgtcagg acggccacca gcaagaaggc cggcaggtcg aagctggagc
     4201 cgtagccgat cgattcgggg atcgtgatcc cgaggtgatc gaggaagatc ccgaggtagg
     4261 ccgaccagcc ctgagccacg acggaggccc cgagcagcag ctccagcagc aggtcccagc
     4321 cgatgatcca cgcgacgatc tcaccgaggc tggcgtacga gaaggtgtaa gccgacccgc
     4381 tgaccgggat cgcggacgcg aattcggcat agcagagcgc cgccaagcca caacagaggg
     4441 ccgcgatgat gaatgacagg atgacggccg gtccggcgac ctgattcgcg gcccggccgg
     4501 ccacggtgaa gatgccggcg ccgatcacga cgcccacgcc gaagacggtc aggtcgagag
     4561 cggtcagctt cttcttcagc tggtactcgg gttcgtcacc ttcacggatc gtctgctcga
     4621 tggacttggt ccgcatgacg ctcatcgggt cactccttcc ggtcggtttg gactggcggt
     4681 gaatctgcct gaacctagcg tgttttcgcg tgtcgtgcgt cacgccgccc ggagattggt
     4741 cgggccaggg atcagggacg gtgtcagggc gggggccgga gcgtcgggat ctcgtcggtg
     4801 tcggcgtcgc gctgcgcgct cttggcggta tgccgcgcgc tcgccggcgg tgcggccggc
     4861 ggcggagcgg cggtggtcct gtgggtcgag gtggccgccc gccgcgtcac gggcggattc
     4921 gcgcgtgact ccttcccgcg gcggcgccgc gccggcgcgg cgctcgggtc cgccccggct
     4981 ccgaagaagt tctggctggc ccggtccccg atgggggagg ccgggctctg ggccggacca
     5041 gaggagttcg aggggcgccg ccgctcggcc gcctgctcgg cccggcggcg cgtggcgccg
     5101 gcggcccggc gagcgctgcg ggcggcggcg gaggcggctc gcttggccgg cggggccgtg
     5161 atcggctggt ccggaccggc ccactcctcg ccgaagatgg tcgagttcag tcggcggaag
     5221 acctgccggg ggttcggggc gtagcgcaac tcccgcagtg tctggtcctg cccggcgctc
     5281 tcgatcgtga tcgtgccgta gccgaagaga cgtccgatga gcggctggtc cagccgcatg
     5341 tcggtgatct tctgcaggcg catcatcggc accgacttgt tgatcacgcc gtcgatccgc
     5401 agcacccggc ggtccgtcgc cgcgaaggag ttgtgccgcc gctcgtacat cttccacgcc
     5461 aggtacgccg cggcggcaaa aaccaggacg aggacgagga aggggagctg gtcaccggag
     5521 atgatgatga cgaacaccgc cagggccccg agcatgatcg cgatcggctt ccacaacacc
     5581 atcgggtgtt gatggacgtt cagccgcagt tcctcatcgc cggtcagggt ccgttggagg
     5641 tcgcggccgg ccggggcgat gaggggatca tcgccggtcg ggccacccgc agccatgcgt
     5701 ggaacggcct gtcagttctg gcgcagcagc gcgtcgaaga actcgaagat attgcggaca
     5761 ccgttgcgca cgatgtccca ggccgagtcg acgatgtcgg ccgcgctgtc gggggaggtc
     5821 aagatcgcat acagcaggaa gatcacgagc agccacagca cgatcttctt gatcgggggc
     5881 atggacacga ctcactcctc ttccgcgtca cccgggcggt ccggggcgca ccacaaccac
     5941 gcctcatgtg gggcaggtgt gaccagtgtg ccggaagtga ctcatgttgc ggaaataccg
     6001 ggcgaaattg ttaccagcgc aaaactaccc gccggtaggt aagacgcttc ggcggttata
     6061 accgccagtc cgtcttacct tcccagcgga gcctccgccg ggtgggctac accaggccgt
     6121 acagccggtc gccggcgtcg ccgagcccgg gcacgatgta gccgttcgcg ttgagcttct
     6181 cgtcgatcgc gccgacgacg agggtgaccg gggggctcac ctcggcgaga ttcttctcga
     6241 gccgcgcgac gccctcgggc gcggcgagca gacacaccgc cgtgatgtcg tcggcccccc
     6301 gctcgatgag gaagtggatc gcatcgctca tcgacccgcc ggtcgcgagc atcggatcga
     6361 gcacatacac ctgccggccc gacagatccg acggcagccg gttggcgtag gtggtgatgg
     6421 ccagcgtcgc ctcgtcgcgc tgcataccca ggaagccgac ctcggcgctc ggcagcagcc
     6481 gcaccatacc ctcgagcatc cccaacccgg cccgcaggat cggcacgacc agcggcttgg
     6541 ggttggacag cttgatcccc gtcgtcggcg ccaccggcgt ctcgatcgcg aacggctcga
     6601 cgcgcacatc gcgggtcgcc tcataggcca gcagcgtcat cagctcctcg gcgagccgcc
     6661 ggaacgtcgg ggagtcggtg cgcttgtcgc gcaggtaggt cagcttgtgg gcgatgagcg
     6721 ggtgatccgg cttgacgaca cgcatacggg aaacttaccc ccatggcgac cgacgacgag
     6781 cagtatgccg cgtggctggg ccacgcgctc acggcggccc gcgctgcgct cgcgacgggc
     6841 gatgtaccgg tgggggcggt cgtggtcgac ccgcacggac agatcatcgg gaccggctgg
     6901 aacgcgcggg aggccgacgg cgacccgacc gcccacgccg aggtgctggc gctgcgggag
     6961 gcggcgcggc ataccgggga atggcgcctg accggctgca ccctcgtcgt caccctagaa
     7021 ccgtgcccga tgtgcgccgg cgcgctcatg ctcgcccgga tcgcgcgggt cgtcttcggt
     7081 gcgtgggacc ccaaggccgg cgcctgcggc tcggtgtggg acatccctcg cgaccggcgc
     7141 gccacccacc gctgcgagat cgtcggggga gtccgcgagg ccgagtgcgc cgagttgctg
     7201 ctcgacttct tcgcggctca ccgcgaggcc tgagcggcct ctcagtcgtc gcgcaggatc
     7261 gcccaggtcg taccgtcgag ccagccgcgg gtgcggtgca gcgactcctt gactagcgcc
     7321 agtcctcccg catgcccgcc gtcatacggc ccagcccggg gtggcgctgt ctgcggtgac
     7381 ggggatttct gccgggcgcc ggccctcggg taccctgctc gacggtggcg tgtccgagcg
     7441 gcctaaggag cgcgcctcga aagcgcgtgt gggggcgact ccacccaggg ttcgaatccc
     7501 tgcgccaccg ccagtcgccg ggggcgaacc cagccgggtt cgccctcggc gccttttcgc
     7561 gtatgcccgc tcaggccggc cggtcctgga tgcgcccggc ccgcgcgaac ccctggtacc
     7621 acgtgccctg attggtcaac atcccggtgt cgccctcgct gagcatcccg tagggctgcc
     7681 cgtccacctg gaactccgcc cgctggccgt cggggaactc gaaggtcgcg taataggtgg
     7741 tccgggtgga cttctcgccg ccggtgacgg cggttcgctt ggccacgacg cgggccggcc
     7801 ggttgaggac cggggctgcg gcgttcgcgg cgtcctccct gcgcttctgg acaccggcct
     7861 tcgcgatggt caccgcgacg aggacgaaga cggcgagcac cattgcgaag atgaggtaaa
     7921 agacgatccc gaaggcgcgg ggcatggcga ctccctagtc gattggggcc ctcgcacggt
     7981 accgcaggcc agcgaggacg ctgcgtgttg ggcaccagtg ctgggtcggc cccagcgggg
     8041 aggccgagtc gacctgccac gcgctgtaac ccggactcct gtgacccgga cgcctgctgc
     8101 gcggttcccg gcgcgaggtg tacaacgctg gtatggatct cgccccaccg cttgacgacc
     8161 tcatcgagcg cgtccgcgcg ctgcgttccg agcctgggga gcgggtgatt ctcgggatcg
     8221 tcggcgcacc cggggccggc aagtccacac tcgcccgggc gctgaccgtc gcgctcaacg
     8281 agagcgcccc cggcaccgcg gtgcaggtcc cgatggacgg ctttcacctg gccgacgttc
     8341 aactggagcg cctcgggttg cgccgggtca agggctcccc gcccaccttc gacgatgtcg
     8401 ggtatgccgt gctcctcgag cgcctgcgcc gcgaccggca gcgcacggtg tacgcccccg
     8461 atttcgagca cgccgtcgag cagcccatcg cggggtcgat caacgtcgag ccgagtgtcg
     8521 agatggtcat caccgagggc aactacctcc tccttcccga tggcgcgtgg ccgcgggtcc
     8581 gggccgcctg ccacgaagtc tggttcgtcg cggtcgagga cgcgatccgc gtgccgcgcc
     8641 tgatcgcccg ccacatccag gtcggcaagg ccccggaggc cgcccaggaa tgggtcatgc
     8701 gcaatgacga ggccaacgcc cggctcgtcc tcgccagcgc cgccggcgcc gacctcacgg
     8761 tgacgatgca ataacggccg tcggccacga cagcaagccc ccgttcagct caccggcagg
     8821 gggcacacgg tgaccggtca cgtcggcggc accgggacct ggaccacctg tcccgagagg
     8881 ttctgcgcaa tcagctgcac cccgggccgg gctgcggacg cagcaagctc cgccgtcacc
     8941 ggcacgccgg gttcgaggac ctcgagcaac gcccgcgccg tgtcgaccgc cgccgtatgg
     9001 ccggccgggc ccgccacgta gcgcaccgcg agcgcgtccg cgtgctcccc gtccgccggc
     9061 cgccgtgtcc gaatcgtcaa ccacgctgtc tccgacgagt ccgccgaatc cctctctgcg
     9121 gcaagcggtt tgccccggcg agcgactttg gccaggacgc cggccaggcg ctccccgtgg
     9181 agttcctccg ccgccaccca cgtcgccgca acgccgaaca gctcaaccgt atgccgctcc
     9241 acctccgcgc gttcaaaccg cccgggatcg gtaacagtct gcgtcgaagg agccgggggc
     9301 ggcgctgccc cgccggccga gggctcgggc aaagtcgacg ccggcgacga gcccgtcgaa
     9361 gcgggggccg acgacgccac cagcacgcgg gcaggcgtga agcagccggc cacccacggc
     9421 gggaactgaa ggatggacgg ggaggggacg acgacggagt cgacgtcgac ggtgtcctcg
     9481 tagtagatcc gttccgccgc accaccattg acgaggtcga gcacacgggg gtcgagtgcg
     9541 gactccttgg gcgcgaactc ccagaggtct tcggtatgca gcacgccgac ggacagcccg
     9601 gcatcgaggc aggcgcgcag ggtggcgacg gacgacgcga ctccgggccg gccgtcgcgg
     9661 aagtcgtcga gcaggacgac gtcgaagtgg gtgggctggg ccaggcgcgg gtcgacgggg
     9721 gattgccagc gggcgggtgc cacgaagcgg cgcggcccgt cggggtcgag gaaggggtcc
     9781 gcgccgtcgc ggatcgcttg gtgccagggc ccatacgcct gccggtaggc gttgcgcgcc
     9841 tcgtgccgcc acccgggcag gaactcgccg aaagagaggg agccggcctc gtgccgggcc
     9901 atcgtcagca cgtccttctt caggtcgcgc acccgccggc cgaaggcggc ctcgaggcgc
     9961 acggcgtact cggtgtcggc ggccttgcgg acggtgtcga agaagccgac ccgcgcgaca
    10021 gccggatgca tccgcaccat cagggacggg gcgttgagga tgagcgagtg gtgcccgggc
    10081 cgggtgagga cgaggtcttc gtcggcacgc acgcacatcg agcgggtgac catcagcgag
    10141 gggtcggcga gcagcggagc gacgccgcgt tcgaggcgct gcgggtgggc ccagtcgtcg
    10201 gagtcgaggc aggtgaagaa ctccgcattc gtctgtcgca gagcggtatt gcgggcgcgg
    10261 taggtgccac cgttgacggc tttgcggatg acgcgcacgc gtgggtccat ggcctcggcc
    10321 gccgcgagaa ctccgtccat cggcgccggg gaggcgtcgt cgatgatgag cagttcccag
    10381 tcggcgtagg tctgctcgac gcaggagcgg acggcagtga tgagcttcgc gtccggattg
    10441 aaggcgctca tgatgatggc gatcaagggc ccgcgaacgc tgcccggagt cgtagcggtc
    10501 gtcgtgaggc ggtcgaaagc cgttggcccg tcgggcaata gcgcgagtgg cgccaggccg
    10561 tcggcgatca gagacgcgtt catcgcggcc agccactcgg cctcggagtg ggtgtcgggg
    10621 gcgaaccgcg gattgcgccg gtcggtcgcg atctggccgc gcacgaggtc cttgacctcc
    10681 tcgtcctggc tcacggcgac cgcccggtcg agctggccgt cgaagagcag gagttgggcg
    10741 taggttcgca cctcgcgccc ggtgaaccgg tggccggccg cggcgagcaa cccgaagatc
    10801 cccagcgccg cgtcgcggtc ctcctgccgg ccgacctgga ccgcgaggac atgggcgtat
    10861 gccgcgagca ccgccttgtc gatgccgcgc ggcaaagccg gggagggccc ttcgccgcga
    10921 tggccgagcc gggccgcggc ccccagggca cgcaacccgt catcgtcgag cgtgccgccg
    10981 gttgccttac gcgccaacac attgcgtccc gcgacggacc gggtgcgcaa gacgctggcg
    11041 acgaaagcca catcgtcatc cgaaccgatc tgggcgatca gccgatcgac cgggccacct
    11101 gcccgctgcg gagccatcag ttccaggacc ccggcctcag gactcgggca gcgcgacggt
    11161 cagcgcgtag ggcgcgtcgc tcgcgtcggt cacgaccgtc accgcgtcac cgatcccggc
    11221 cgcttccaac cgctcgcgat cggcgctgtc ggcgacgaca gcgcggacct caagaccatg
    11281 ggagagagca gttttggcgg cgctgacccc cttttcgtca agccggtgaa ggagcacgac
    11341 cgagccgtgc gggagggcgg catacggatc gtcgtgggcc gccccggtgc cgatgcgcgc
    11401 ctcaaggtcg gccgtcgccc gctccagcga gctctgcgcc cggtcgagcc ggccgacata
    11461 ctgcgcctgc aacagccgga tggtcccggt cagatcgtgc tcgcccgggt gggcagaccc
    11521 ctgggccgaa cggggaccgc cggccaccgc cgagcggagc ttggcggcat cgctgcgctg
    11581 agcgcgcacg agcaggttgg tattgagggc gacgacgccg atgacgccga gcatcgcggc
    11641 cagtccggcc accgccagcc accaccacga cgcgatcgcc ccgatgaatg ccagggcccc
    11701 gagcccacca agggccgcca gtcccatcag gacgcgcttg ttggtcatct cgtggctgct
    11761 ccttgggggt gggcctggca tctagacttt cgaagtattt cacggcccct gcagaagagg
    11821 acgtatgccg caccccgcag ccgaactcgg gctggcccgc tcctggctgg tctcgcgaga
    11881 cctcaccgac cgcgagatcg caaccctcgg tgagacggcg ttgcgttgcg ccacacccgg
    11941 attgcgcgac gtgctcgcgt tgcgcgcaac cggtggcggt atgacggccg atgaggtcgg
    12001 cgcccatctc gacagccacg gccggtggcc gccgggagcg ctggaccggc cggccgcagc
    12061 ccttgccctg gcctgccaat gggcggtcgg gggcgaacag atccgcaacc agccgcgcgc
    12121 ccgggttgcc ttcgccgacc tgctggccac ctgcgggccc ggcgtgttcg cgcagatgaa
    12181 acgcagcgag caactcgccg tccaattgcc ttggttcgca ggggatttca ccgaggcgcg
    12241 cgatctgatg cgcgctcttc cgctggcgcc ggaggccgca gccgacctcg agaccgatct
    12301 cgccaacccg tgggcgacgg gtgtggagcc gacaccggac cgcgtgcgcc agtggctcgc
    12361 attgttcaac cgccgcgtcc gcgacgccgg cctggccccg gtcagcgtcg ccgccgacgc
    12421 cgacaatccg ttcgatgcgc tcacggcgcc gaccgggccc agcgtcgacg ggcccctggt
    12481 gaccgtcatc atgccgagct acgcccccga cgccgggctg ctcaactcca tccgatccat
    12541 ggccgcccag acctacggca acctcgaggt gctgctcgtc gatgacgcca gcggaccggc
    12601 atacggcggc ctgtatgacg aggcgtgcgc cctcgatccg cgggtgcggc tggtgcggat
    12661 ggcccgcaac ggcggcagct atctgggccg caacgccgct ctggcgcagg ccaagggccg
    12721 ctacatcacg acccaggacg ccgacgactg gtcgcacccg gaccgcatgg cccatcaggt
    12781 cgccctcctc gaggcgaacc cgctggcccc cgcgagccgc agcgacgcga tccgggccct
    12841 cgacgatctt tccctgcagt ggctcggcta cgactcgcag cgccgcaacg cctcgtcgct
    12901 gatgctgcgg cgctcgaccc tcgccgcgat cggcggtttc gacaccacgc gcaagggcgc
    12961 cgactccgag atgcacgagc ggctcgagcg gcggctcggc cccaccctgg acacgatgac
    13021 gccgctggcg atcacccgat tgcggctggg gtcgttgtcc cgcagcgatt ttcgctatca
    13081 atggatgtcg ccggaccgcg tgctgtacag cacgtcatac cgtgcctacc atcgctcgct
    13141 gaccccgcag acgcccccgc tcaacccggc cgagcaggtc cggcaccgag cgttcccggc
    13201 gccccgggcc taccagcggg ccctgcccgg cggagccacg ctccccgagc ggtatgacgt
    13261 cgtctacctg ctcgacctca gcctccccaa cgccctcgac ggtgcggcgg ggaccaacct
    13321 gctcgggcgc gggttgcgcg ccgcgatgat ccaccaggag gccgccggtg tcggcccgcg
    13381 ccggcgaccg atgacgttcg gcacggcgat ccagcacgcc ctcgaccggc gggtcgacct
    13441 gatcagcacc gtcgacgcgg tgccgtgcga actgctcatc gtgctgacac cagaagtcct
    13501 cgaactgccg agcgaggatc ggccccaggt ccggcctcag cacctggctg tcctggccac
    13561 cgccgacagt gagacccaga tcgaccagct ggccgtgctc gaaggcgccc gtgaggtgtt
    13621 cgacctgccc gtggagttcc tggtcggcaa cctgcgcgag gccgaggagt ggcagcaggt
    13681 gaccggcgag ccgacccgca cggtccgcgg agccctcggc gagctggccg cggccaaacg
    13741 gtccgccacg gagacgccat gacgaccgcg ccccaggctc ccgccgtcga gacccgggct
    13801 cccgccgtcg ccaaccccgc cgacgccaac cccgccggcg caaccggcgc cgtcggggcg
    13861 ccgaccccgc gcaccgtcat ggtgctcggc gaggaggacg cggcgctgcg gcacctcgtc
    13921 acccgctggg cgctggtgca tcccgaccgg gaccgcgacc gcctcgtcgc cgttcatttc
    13981 ggcaagacgc ccgccaagct gccgccgggc gtcgtgacca tcgccgtcgc cggcgggggc
    14041 gcgatcctcg acggcgcccg gcgcgccggc cgcgccaaac ggctcctcga cgccctccgg
    14101 agcctgcctg cgggacgctc cgctctgcag gacgtcgaca cgctcgtcgt ccccgacgcc
    14161 atcgtcgacg acgcgcgcgc cctcgcggac ggccgcaccg tgcaggcgtg gtcggccttc
    14221 gcggcccacc tgcgcgccga ccatctggcc gctctgttga cagcttcggc cgccggtccc
    14281 agccagggca ccggcactgc cgtcctcgtc gccgacgccg tcgacgccct cgccgacacc
    14341 ggtgccgacc cctccggcat cgctgcggct gccgcccgcg ccctctcccg cctcctgcgc
    14401 gccggagaca ccgaggtggt cgcgagcgtc gccgattcgg tgcgcgccct gccgcacgcg
    14461 acccgcgatc gtgtcggcct ggacgcgatg ctccgtatcg ccgagatctc gatgggcggg
    14521 ccggccgacc ccgccgccga cgccgctgcg gccgccgcct gcttcgcggc cgcggagcag
    14581 cgcaccgtcg atggtgacgt cagcgacgtg ccgatgctcg tcaccgccgg cctgcgcctg
    14641 atcttccacg gcgatctgca cagcgacgga ccgcattccc ccttgatcga cgaccccgtc
    14701 gggcagcttg cgccctggcg ccagtcggcc ttcgccgccc gcctccggcc gaagccaccc
    14761 gccgctgacg ccgacccggc cgacccggcc cagnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnngacccgg ccgacccggc ccagccggcc gcccggccga agatcgtcgt
    14941 cctcggcggg gccttcggcg ccttctatca acccatcgtc gacctgctgc gcgaggccgg
    15001 tcaccacccg acggtcatca cgcccgaccg cctcggcgtc gccttcaccc gccacaacct
    15061 gaccgacgcg gtcgtcgggg cctggctgcg gcgcgtggac ccgcagctgt tcggggacct
    15121 gccgctggac ccgggcgtcg cctcggcgat cgccgccctc gagaccgcga tcagcggggc
    15181 cgacgtcgtc ttcgccgact ggtgcgacgc ggaggcggcc ttcgccagct ggctcctgcc
    15241 accgcacgcc cggctcgtcg tgcgcgtcca ccgcgtcgac gcggtccgcg catggcacca
    15301 actcgtcaac tggccccgcg tcgccgccct gatgttcgtc agcgatcacg tcaaatccgt
    15361 cgtggagcgg cagctgacgg acccgggcag cccgtcaccg gtgccgatga cggtgctgcg
    15421 caacgtcatc gacgtccacc gctaccagcg cccgaagacg cccggagccc accggcgcat
    15481 cgccctcgtc gggtggggcc ggcgcgtgaa ggacccgatc ttcgctctcg acgtgctggc
    15541 cgacctcgtg gccgaggacg acacctggca gctccacctc atcggacgcg atttcgcggc
    15601 agccacctcc ccggtgctcg ccgactacat ccgcagcttc cgcgagcgcg ccctcgaccc
    15661 ggccatccgc gacaacatcc ggtgggtcgg gttcaccgga cggctcgagg aggccctcga
    15721 cgagtgcggc tacgccctat cgacgagcaa tgtcgagggc tggccggtcg gcgtcaccga
    15781 ggccgctgcc tccggctgta tcccggtgat ccgcgactgg ccgcaggtgg ccgcgctcgg
    15841 cggggcccgc gagatctatc gcgaccacct cgactgggtc gtcgccaccc ccgccgaagc
    15901 cgcggcccgg atccgcgccg ccgccgaccc ggacgcctgg gccgccgcgt ccgcggccgc
    15961 gcgcgccgcc gccggtgagc tgtgctcggt cggctcgacc cgcgaggact acctcgcggt
    16021 catcctcggg ccggaaccgc tgcccccgcc agccggctga acagcgccgc cacggcgctg
    16081 gggtgctggt ccggcacgtg gtgcgccacc gggagccggg tcaggggcga gtcctgtggg
    16141 acgagcagga tcaccggggt gccgtatgcc gcgtggtcgc cgatctccgg caggtcaccg
    16201 agcgcgtagg gcagccccgc cggccggggc cgatccagca ccagcgcgta gtcgcccgcc
    16261 gctgccgttg cggcgctcgc ggccgggccc aggtccggag cgaggctgac gagtccctcc
    16321 agcccgctct cgcgcagcag caccgccacg tccgccggga cgttggtgag caggtgcagc
    16381 cgcggccgcg ctgcctcggg aagcaggccg tatgccgtca ggaccggctg cagctgcgcc
    16441 aaccggtccc ggtccacccg ggccgtcacc ctgacatccc cggattcccc ggccccgccc
    16501 ggtgcggcgg tgacggggca ggcagggcgg gaccacacct ccatacggtc cgccagccga
    16561 tcccgcgacc cggcgccggc tcgcgcgagc aggtgctcgc ccagggccgc ggtggcgacg
    16621 acgacccggt cggcgcgcgc ggccaccacg gcaagcgccc aatccccgag gaagtcggag
    16681 accgggtcgg cgtcgaggag ggcgcggcag gccgtgcgca gcggccccgg cgagaccgtg
    16741 gccgtcagcg cccggcccga ggccagatgg ttcgggggat ccgtcagatg ggccgtccac
    16801 cccggcacca ccgcgcgctc ccggagcagc gcgagcacga gatggtccgc gagtcccgcg
    16861 ggacccgaat acgcgccatc ccacctcacg cccgccgcct gccaggcccg caccgcaccg
    16921 gcggcggccg cggcatacga ctcgattccg gcccaggtgc tcggcgcgac gggatggccg
    16981 ggcaggctga tcgagcgtcc gatcaggtcg tcgacctccg ggcgcggctg ctcgcgcgga
    17041 cgcgtgtcgt cgaggtcggc ccgcacgaca tccaaccggg tggcgccgtc ggagaggagt
    17101 cgcgctgccg cggtcgtggc cgcctcgtcc cgccacgggg aaaaggccca cgaactcacc
    17161 aataccgagg tcatgccgct gattgtcccc gctgcgggcg cccggtcggg gacaggggcg
    17221 gtcaggaccg cgtgtcacaa tgcccgttgt gcctttgcgt gtccctgcta cgctccccgc
    17281 ccctccccgc cgccgaaacc cccgtcgcgg cgccctgatc gccgccggcg ccgttgcggt
    17341 catcgtcagc cccgtgttgg cgagcgcagg cccggccgcc gcggacggcg agcggactgc
    17401 cggcccggtg caggcggccg cgctgacgtg gacgaagccg accttcgtgc gcacgatcgg
    17461 cgctcgtggg tccgcggggc tgtatgcgtg gggcgtggcc tacaacccgg tcaccaacca
    17521 gaccctcgtc ggcgactacc gcaacttcca gatccggcgc ttcagcagcg gcggcaccct
    17581 gctcggctcg ttctaccggc ccgcctcctc ccggcgcggg gtgcccgagg gactggccgt
    17641 cgacccgcgc gacggctcgg tctatgtctc cgaccactcc aaggacaacc gcggctatgt
    17701 cgcgaagttc tccaaggacg gcaagttcat ctgcgagttc aaactgacct ccacctatca
    17761 ggcgtggatg gcgatggacg ccaacggcta cctctacgtc agcgattccc acgtctggca
    17821 caactccacc aacccgcccc aggtacgcaa atactccctc aacgacacca ccgccaccgc
    17881 aaccgaggtc gcccacttcg gttcctacgg ctccggcgtc ggcgagatcc ggcagatcac
    17941 cggcctggcg atcgacgaca agaccggcaa aatcgccgtc gccgacacga tcaacaagaa
    18001 ggtcaacgtc tacgacggca gcggccgctt ccaatacacc ttcgccggcg gggtcttcaa
    18061 gggcgacctg cgcggggtgg ctatcaatgc gagttcgcgg gtggcctatg tcgtcgacgc
    18121 cagcgccgga caggtcgagc gcttcaatgt cgacaccggc gcctcgctcg ggcatttcgg
    18181 gtcgctcggc accggcgcgg gccagttcgc cgacggaggc cgccagatcg ccatcgacgg
    18241 cagcggcaat gtgtgggtgg ctgactacgg caacacccgc gtcctgaagt tcaccgccac
    18301 cggcgggttc atcggtgctt accccaaccc gccccagaac gcgcccaagg ggtcgttggc
    18361 cctcgtgcgc gatgtggcga tccagccctc gacgagacag atcttcacga tcgagcagga
    18421 caaccacagg atgcaggcct tccggcccga cggcaccccg gccgggatgt ggggccgacg
    18481 cggcaccacc ccgccgctcg ggttcaacta cccccgcggc ctcggcatcc agcccggcag
    18541 tgagcggttc tggatctcca acaccaccga gcaccagatg cgcgtgctca acaaggacaa
    18601 gtccctcgcc ttcaacctcg ggctgccgtc gggcagcccc gcgaaccact acaacgagcc
    18661 gatcgacgtc gagttcggca acggccaggt gtatgtcggc gacgacgccg accggaatgt
    18721 caagatcctc aacgcccaga ccgggcagga ggagggccgg ctcaatgtcg cgggggcggg
    18781 ggtggccgtc gacccggcca cgggcgacat cttcgtctcg tcgtggcgcg accggcgcat
    18841 ctaccactac accaagaacg gtgggcaggg ctcacctgcg gtgatcggtt cggcgggcac
    18901 gggcgccggg cagttccaga agccctggga catcgacatc gtcggcggga tcatctacgt
    18961 caccgacgcg acccgcaatg tgctcgtggc gtatgagcgt gacggcacct tcctcggcga
    19021 gctggggtcc gccggcactc ggccgggcca gttcaacaac ccctccggcc tgacccacga
    19081 cgcggcgggc aagatctatg tcgccgacgc cggcaacgac cggttgcagg tcttcgacac
    19141 caagggctgg gtcaagtccg acgcgaaccc gccgtcggtg tcgatcgcgg cgcccggagc
    19201 gggcgctacg gtcggattcc ccgtcacgat caccggttcg gtcaccgaca acctgcgcaa
    19261 tggcacggtc gaggtcgcgg tccaggaccg caagaccaag ctgtggtggg acgggcgcat
    19321 cgccacgtgg agtgcgacga aggtctacat caacgccggc atcaagggcg gggacatcag
    19381 gaaccagcag tggtggttcc cactgatcgg cgcccaggcg cgtgccggct atgccgtgtc
    19441 ggtgcgcggc gtcgacgggg ccggcaatat cggctcggtc gtcacccgct ccttcaacac
    19501 cggcagctga ccccgctgct ccggcgcggc ctcagccggc cgtggcggat gagcacgtcg
    19561 gcagcggcga gagttcggtg aacttgtcgc cgatgacgag gtcgatcgtg ctgtcggtcc
    19621 gcttgtcgtt gacgaacttc gcgtcgttga gggcgagccg cacggcccgg gcgaagggga
    19681 cgcccggcgt gccgtagcgg atctcgcccg tgcccttgac cttcttcttg agggggtcgt
    19741 tgtcgatctc gacgaccgag tagccgcgtt cgcgtagttg accggccact ttcgcggcca
    19801 acccgggcgt cgacgtggcg ttgtagacgt tgaccttggc gtcggagacc ttggcgatcg
    19861 gcttctgcgc ggtgcccgtc atacagtcgc tggccttggc ggactccggg ctggtgtcct
    19921 cctggatgta ggagtaggcc ttgtagaagg cgccaccgag gaaggccagg gccaacagga
    19981 gcaccacggc ggcgcggatc tgccggaccc gccggggagt tccggcggtg agctgagcgg
    20041 ccattgctct gcgggcttcc tctcaggagc gtgcgggccg ggtcggacca cggactacat
    20101 tgactgcgtg actgaaacac cttcccagac cccgcccgcg gatgcgaacg gagaccagga
    20161 gacggctacc gccccttccg acgccgcatc ggcgccgcag gttgggtccc cggctcagcc
    20221 atccgtcaag ggtagcgtcg ccgagaacgt ggatgcgccg gcggacggcg gcgcggcggg
    20281 taacgctctg gcatcggaga tccgcgacgg ctacgccttc gaaggcccgg ccatccagtt
    20341 cggtgcggcg gtcgtcgacg ggcaggtgta tgccgacgct cccgtccgca tccccctcgc
    20401 cgtgatgaac cggcacgggc tggtcgcggg ggcgaccggc accggcaaga ccaagacgct
    20461 gcagctgatg gccgagcagc tggtcgagca gggggtgtcg gtcttcctcg ccgacatcaa
    20521 gggtgacctg tcgggtatgg ccagtcccgg tcagcccaac gacaagatca ccgcccgcgc
    20581 acaggacgtg gggatggcgt ggacccccac cgcatacccc gtcgagttct tctccctcgg
    20641 cggcttgggc aaagggatcc cgatccgcgc ctcggtgtcg accttcgggc cggttctgct
    20701 gtcgaaggtg ttggggctca acgccaccca ggagtccagc ctcgggctcg tcttccacta
    20761 cgccgacacc aagggcctgt ggctcgacac gatcgaagac ctgcgggccg tcatccagtt
    20821 tctcaccagc gacgccggca aggccgatct gaaggagttg ggcggcatct cctccgcgac
    20881 ggcgggggtg atcctgcggg agctgatcaa cttctccgct cagggcgcgg acgtcttctt
    20941 cggcctgccg gagttcgaca ccaacgacct gctgcgggtg gcctcggacg gccgcggcgt
    21001 gctgtcgatg ctcgaattgc cggctgtgca ggaccggccg gacctcttct ccaccttctt
    21061 gatgtggctg ctggccgacc tcttccacga cctgcccgag gagggcgacc tcgacaagcc
    21121 gaagctggtc ttcttcttcg acgaggcgca cctgctcttc aacgaggcga gcaaggactt
    21181 cctcgacgcg atcgagcaga cggtccgact gatccggtcc aagggtgtcg gcatcttctt
    21241 cgtcacccag tcgcccaagg atgtgcccgc cgatgtgctg gcccagctcg gcaaccgggt
    21301 ccagcacgcc ctgcgggcct tcacgcccga cgaccagaag gcgctgcggg cggccgtcaa
    21361 gacctacccg cacacccaat acgacctcga ggaactgctg acgagcctcg gcatcggcga
    21421 ggccgtcatc accgtgttgt ccgagcgggg cgcgccgacg ccggtggcgt ggactcgcat
    21481 gcgggccccg cagtccctga tggacccctc gcccaccgag ctgatcgatg cggcggtgaa
    21541 cgcctcgccg ctgacgccga agtacctcac cgcgaccgag cgcgactcgg cgtcggagat
    21601 gctcggcaag aaggtcgccg aactggacgc cgcggccgcg gcggccgccc aggaggccga
    21661 gaacgccaag gtccgcgagg ctgccgcccg gcaggcggag aaggaccggc tggccgccga
    21721 gaaacaggcc gagaaggacc gggaggcggc cgcgaaggcc gccgccaagg aggccgccgc
    21781 agcggagaag gcggccaggg cggacgagcg gcgccgtgag gaggaccccg gcgtggtggc
    21841 ctccgtggtc aagtcgggtg ccttcaaatc gatgctgcgt tcggccggca ccgtcatcgg
    21901 acgcgagatc acccgctcga tcttcggcac cgcgcgccgg cgctgacgcg ccgcccgctc
    21961 gcccgaccac tcaggccagg agtgcggtga tgagcgcgat gaccggaacg ctgcccaacg
    22021 tggtgaccag aatggtgtcg cggcaactcg ggctcggctt tcgttctcgg gcctggaaat
    22081 aggctggccc ggggacgaaa acctggcccg tgtcaccgac aaccaggccc ggcaacgaca
    22141 gccaggcccg acagtgcgcg acacgtccag ttcatcgaag gactggaccc cgttcgtccc
    22201 tgacgcatca ctacgcttac cgcagttgcc catcggcagg gggtgctgca ccgagcgccg
    22261 acgcaacagg ccagcccaac gccagcaaga cagtgacggt gcccgagaga tgtcccgacg
    22321 ttccgccccc gcgcgctccg tgctcgcgct gcttctcacc gcgctgacgg cattcgccgt
    22381 cgcggccggc gccggcccgg ccacgggctc ggcctccggc tcggtctccg gctcattctc
    22441 cggcgaggcc cgagcggggt cggtcacctc ctatgtggcg gagcgcatca tcggggcgcc
    22501 gtcgttcccc ggggtggccg cgtgggggct cgcctacaac ccggtcagcg acgaactcat
    22561 cgtcggcgac tacgtgtcgc gacaggtgcg ccgctacacc cgcggcggga tctacctcgg
    22621 cgacctgcgc aacgccggca agaccatcgg cggcgtcgcg tcggccctcg gcgtcgaccc
    22681 gcgcgacggg gcgatctacg tcgccgtcac cggggagggg cgctcgtcgc tcgatgtgcg
    22741 taaatacgac aaggaaggca acttcctctt cgacctcgac atgcccgggg cggccacctg
    22801 gctgaccgtc gaccacgacg gcaacctgtg ggtgccggag gggttctccg gttcggtcgt
    22861 gcgcaagttc accgtggacg acgccaccaa gtcggcgcag ctacaactgc tgatcgacaa
    22921 ggccggtccc ggcacgacgc tgaaggtgct caccggtgcg gccgcagacg cggcgggcaa
    22981 tatctatgtc gccgacgtcg gcaacaaggt gatccactcg tggacctcga ccggggagtg
    23041 gcgtttcgac atcggcccgg acccgatccg cggcgacctg cgcggcgtgg ccatcgacga
    23101 cgaactgggt cgcatctacc tctccgacgc cgtctacggc ggcatccgct actgggacat
    23161 ggacggcaac taccagggcg tcatctccgg cctcggcctg caggacggcg aattcaccga
    23221 cggtgcgcgg cagatcgccg tcacccccga ccaccacgtc tacggcgcgg actacggctc
    23281 gttccgggtg caggagttcc tgccggacgg cacattcgtc aaggtgttcc cggaccccgt
    23341 catgcggccc gacccggccg gcatctcgca ggcgcgcggc ctggacgtcg acccggtcac
    23401 cggcgatgtc gtgaccgtgg acgccttcgg gcagcgcgtg aaccggtatt cggcccaggg
    23461 cgagctgctc gaccagtacg gccgccgcgg ctccagcccg ccggagggca tgaactaccc
    23521 caaggccgtc gccgtcgatc cggcgacccg cgacctttgg gtgtcgagct tcgaaggacc
    23581 tccgggcgtg gtcggctaca ccttcgactt ctcgtccgtc gtcggccggc cggacatccc
    23641 ccggtgggtc ggtgacctgg agttcttcgg cggcaagctc tatgcgctcg aacgccgccc
    23701 gggtgcggtg cgggtgatcg acccgaagaa ctcgaaggtc gaacggacgt ggctgacgag
    23761 cgtcggcctg ctacacggcc tcgccgtcga tcccaacaac ggcaatatgt ggatcaccca
    23821 cgactccaaa cccgtgatgt atgtcgtcac gccggccggg cggatcatca ggacgatcac
    23881 gctgcccggc gtgggctggg gagtggcgat caagggcgac gaggtcgtcg tcgccgacac
    23941 caccacgggg tacctcgacg tcgtcagccg cacgacctac gccgtcaagg gcaagatcgg
    24001 caagggcaac ggcttcggtt tcgggcagct caagacgccc tccgggctgc agttcggacc
    24061 cgacggcaag ctctatgtca tggagcaggt cagctcccgc gtgaccgtct tcgctcccgg
    24121 ccccgcaccg gccgcggaga ccgtcaaacc ggtcgtcaag atcgacgccg ggccgatggt
    24181 gaccggaggg cagatcgtcc tcaccggcaa ggccacggac gcatcgggca tcgcgttcgt
    24241 cgaggcgcgt gtcaaggacc tcgccaccgg ccgcttctac gacgggaagg ctgcgaccta
    24301 cctggcgacc tcgacctggt cacccggcgt ggtctggggc gacctgaagg cggcgtcgtg
    24361 gcgctacacc gtccccgcca cgctgccgag ccgctcgtat gccgtgacca tccgcgccac
    24421 cgatcggcgc ggcaatatct ctctgctgct caaccgcacc gtcgccgtcc cgtaatcacc
    24481 ccggcaccgt aatcaccccg gcgccgcttc ggcgggtggg aaccggccgg cccgatcggc
    24541 gaccggctca gtgctgagtg gccttcaggg tgtcggtcag ggcatcgagg acgccgacgt
    24601 cctcgatcgt cccggggatc gagggcacct tgccgtcggc gatctcacgc atcgtcttgc
    24661 gcaggatctt gcccgaacgc gtcttgggca gggcggcgac gatgtcgacc tgcttcagcg
    24721 acgccaccgc accgacctcg tcacgaaccc gctggatcag ctccttcttg atcttctcgc
    24781 cctcgagatc ggggtcgtag ccgcccttga gcaccaccag cccgcggggg atctggccct
    24841 tgaagtcgtc gtgcaccccg atgacggcac attcggcgac ggccgagtgc ccggcgaggg
    24901 cggcctcgat cgagccggtg gagagccggt gcccggcgac attgaggacg tcgtcggtgc
    24961 ggcccatgac gaacacatag ccgtcctcgt cgatcatgcc gccgtcgccg gtgaggtaat
    25021 agccggggta ggccgacaga tagctcgtga cataccgctc gtcgtcctgc cacagcgtgg
    25081 gcagcgtgcc gggcggcagc ggcagcttga tcgcgatggc gccgtcctgg ccgcgcgggg
    25141 cgtcctggcc gtactcgtcg aggacttgca cgtcataccc cggcactcgc accgatgggg
    25201 acccgggctt gatcggcatc gggtccagac cgcgcaggtt ggccgcgatc ggccagccgg
    25261 tctcggtctg ccaccagttg tcgacgacct cgcagtgcag cacgtcgttg gcccagtggt
    25321 aggtgtccgg gtcgaggcgc tcgccggcga ggaagacatt gcgcaggtgg gagatgtcgt
    25381 gcccctcgag cagcgccgcc ttggggtcgt cgcgcttgat ggcccggtat gccgtgggcg
    25441 cggtgaacat cgccaccgca ccgtattcgg cgatgatccg ccagaaggcg cctgcgtcgg
    25501 gggtcccgac gggcttgcct tcgtagagga tcgtcgtcgc gccggtgagc agcggggcgt
    25561 agacgatgta ggagtggccg accacccagc cgacgtcgga ggcggtgaac cacacctcgc
    25621 ccggcttgac gtcgtagacg ttctccatcg accagcgcag ggcgaccgcg tgcccgccgt
    25681 tgtcgcgcac gatgcccttg ggccggcccg tcgtgccgga ggtgtagagg atgtagagcg
    25741 ggtcggtcgc cgcgacctcg acgcagtcga ccgggtcggc gcccgccatc agttcgttcc
    25801 agtcgatgtc gcgggcggtc atctcggcct cggcctgcgg gcgctggagc atgacgaccc
    25861 gctcgggctt gtgcgtcgag cgctcgatgc cggcgtcgag catcggcttg tagggaatgg
    25921 tccggttggg ctcgatgccg cagctggccg agacgacgac cttgggggcg gcgtcctcga
    25981 tccgggccga cagctcggcc ggcgcgaacc caccgaacac gaccgagtgg atggcgccga
    26041 ggcgggcgca ggcgagcatg gcgatgacgg cctcgggcac catcggcata tagatgacga
    26101 cccggtcgcc cttctcgatg ccgaggttgc gcaggccacc ggcgaaccgc gaaacctgtt
    26161 ccagcagttc accgtacgtg tactcggagc ggtagcccgt cacgggcgag tcgtagaaca
    26221 gtgcgatctg cccgcccctc ccgtcgcgca cgtgccggtc gagcgcgttg aagcaggtgt
    26281 tgagggtgcc accggcgaac caccgataga acggcgggtt ggagtcgtcg agcaccttct
    26341 ccggcggcgt gatccactcg atcgactcgg ccgccttgcc ccagaaggcg ctcgggtcgc
    26401 tgatgctctg ctcgtatgcc gccgcgtagg ctccattgct cataccgcca tcgtggccct
    26461 cccctctcgc tcgcgccacc ccggggtgga tgacttaggt cagcggccgg tatgccgcgc
    26521 ccagcggggc gcagtccggt gacccgagcc ggttgtacgg gcgccggccc agctcgacgc
    26581 cagcgactgg gggagcagcc gtggtcgccg gcgccgagcc cccctgctaa gggccgccgg
    26641 ctccggcatc gggccgtgac ccggggcgtc gcggcgacgc gatgctcgca tcgcgttgcg
    26701 cggcaacggc ttcccgcacg gcgggagtct gcgcggcgac cccggctgcg gtcatcgtga
    26761 cgacctccca gtggtcgggc atctcgagtg ttcggctcag gccctctcgc tgagcacgtg
    26821 atcgggcgcg aactcgcggg gactccaacg ccgcagcagg acgggcaaca gtgcggatgc
    26881 gtccgccgag gaatcgccgc ctgtcgcatc gctgtcggca acatcgtcag gatggggagt
    26941 ctgcacgacc tgagacagta gccgggcatg tcggctctag cctggacgtg gaaggaggcg
    27001 gacgatggct gaggcgaccg tcggggtggt cggcggcggg atcgtcgggc tggccgtggc
    27061 gcgggcgctg acgaggcgcc ggccggggat ccgagtggtg gtctacgaga aggaggatcg
    27121 cctcgccgcg caccagacgg gccacaactc cggggtcgtg catgcgggca tctactatgc
    27181 gccggggtcc gccaaggccc gcctgtgcac gcgcggccgg gggctgctcg aggactacct
    27241 cgccgagcac gatctgccct atcgccgctg cggcaagctc gtcgtggcgc tcgacaacga
    27301 ggagctgggc cgcttcgacg aactggcgcg tcgcgccggc gagaacgggg tccccgggct
    27361 acgccggatc gacgccgccg agatccgcga gatcgagccg tatgccgtgg gccgggccgc
    27421 cctgcactcg ccccggaccg ggatcaccga ctatgtcgcg gtggccgaag ccctcgccgc
    27481 cgaggtccgc gccgccgggg gagcggtcca cctggcgacc ccggtccgcg cgatccgggc
    27541 ccgggcgagc ggcgtcgagg tcgccgtccc cggcgcccgg cacggcctcg acggcctcat
    27601 cgtgtgcgcg ggtctgcagt ccgatcgcgt ggcccgtctc gccggcggcg cccccgatcc
    27661 acacatcgtg cccttccgag gcgaatacct cactgtcgca acggaaaagc aggagcgcgt
    27721 ccgcggcatg atctatcccg tcccggaccc gcgctacccc ttccttggtg tccatttcac
    27781 ccgccgcgtc tccggggaac tcgaggtcgg ccccaacgcc gtcctcgcgc tagcgcgcga
    27841 aggctacggc tggaccacgg cgagcctgcg cgacctgggt gagctggccg cataccgcgg
    27901 catgtgggga ctggcgaagg cgcactggcg caccggtttc gaggagatgc gggggagcct
    27961 gtcgttggcg gcatacatgc gggcggccag ccgctacgtc cccaccatca cgcgcgccga
    28021 cgtatgccgc ggcggcgcgg gggtgcgcgc ccaggccgtc ggcgcggacg gcacgctcgt
    28081 cgacgacttc gtcgtcgagc aggaggacgc gatcaccttg gtgcgcaacg ccccctctcc
    28141 cgccgccacc tccagtctcg ccatcgccga gtatgtcgtc gacggcctgc ctggccgctg
    28201 acacatgcga aagggcgagc cgggatccgg ctcgcccttc tcggccatgc ggctggcgga
    28261 ggatgcggga tttgaacccg cgaggggttt catccccaac acgatttcca atcgtgcgca
    28321 ctaggccact atgcgaatcc tccgccggag agggtacctg agggcggggc cgcggccgaa
    28381 atcggcgggg ccgggtgcgg gggccggtcg ccccaggcgg gcgtggaggc aaggacaccc
    28441 cgcgtacctg gaagagctca cttacccttg ctgcattcct gccctggggg agttgggtga
    28501 ggtaccacca cgcggggtgc ctgccgcaag tataggtgcc tgctcgacgc agccgcgcat
    28561 caccgacgag tgcgggcctc aaccggcgag gtggttgacc agggcccagc cggggtcggg
    28621 ggtgaccacg gtctgcgcgt cgccctggtc ccactccgcg gccgtcgcgg cgacccggtc
    28681 ggcggcctcg gacgtgccgt cgacataggc atgcagcagg cgcacgccgt cgctgctctg
    28741 atgcgcgacc acccgcccgt ccgccccgag cacctcgctg agacggtcct cgagcccgcg
    28801 cagggcctcg agcgagcccg gcccgggcag cccgtcgggg gtgcgatcgg catacggcac
    28861 ggtgacggcc acgtgcgtgt cgaacaaggg aaaggtgatc tgccgcaacg gaacctgcgc
    28921 cagcgcgagg acgggcgaac cgctcgggcc ggtgccctgg agcaccaccc actgcggctc
    28981 gccctccgcg gtgcgaaagc ccgccgcgaa gtcggccacg acggtgcgca ggtgctgcag
    29041 cgggaaggca tcgagcggcg gctcgccggg ccacgacacc tcgccgatcc acgtctcgac
    29101 catctcctcg ccgaggaagg agtcgagcgc gagataggtc agctgagcgg cctcctcctc
    29161 accgatgtcc atgaacaccg ggtgctgcac ggccacatcg atcgccgtgt tgccccggtg
    29221 ggccgcgacg acgaagtccc cgaggcgcaa gcggcgcccg gcgaactcga gctccgtgtc
    29281 ggcggcgtcc gggagcggtt ggcgcagatc ggcatacgac caatcggggg cggggacggc
    29341 ggcgcgcaac cacgcccgcg ccggcgcccg caactcggga tccccggccg ccgtgacgac
    29401 gagcacgtgc cccccggccg gacccggggc gaactcccac tgcaggcccg cgccgatggc
    29461 ggcgatgtgc gccgacatct cagcgaccag gtcgtctttc gggccgccgc cgatgacgga
    29521 ctcggcgagg ccgcgcccgc gctggcccca ccagtcccag aacgccgcga cggcgctatg
    29581 ccgcccgctc acctcgttca tatcgagcgc gccatcttgg gcacgaggag gaaggcgagg
    29641 aggtagagcg cgcccgtcac caacagcagg ttgttgtagc cggtgaccag ggctgcatac
    29701 tccaggcacc cgccgacgag ggcgccgagc agattgaacg cgaacgccga ctgcgactcg
    29761 gacgattcgg cgaagcgctt ggcgaaggcg acattggcca ggaagatcgg caggaacgcc
    29821 agcgccgtcg ccaggatcag ccgcggcacg acgggaagcg acagcagccg cccgggctgg
    29881 accacccagg cgagggcgag agcggcggcg atgagggcgt agacgacctt caggggcggg
    29941 gtgcggaagc ggcgggtggt ctcgaccgcg cacaggaccg tcaggagcac ccccgcgaag
    30001 acgagcgcgt tgacgaacca ggtggtgccg aagagcaggg cgaagctggc gatgttcttg
    30061 gtctcgagca gcatgaacgc cgcgcccatg aagaacaggt cggcataggg ccgcatcccg
    30121 cggaaggggc cgccgagcgc gcggatggcg atgagcgagg cgaggaggat gccgccgagg
    30181 gtgatgaggt agagccgcgg gaaggtgccg cccttgaagt agaggaacgg cgcgttgtcg
    30241 gtcgccggcg cgatgacctc gcccgcgggt tgccaggccc cctcacagct ctggttcgcc
    30301 tcgtcgacgg cgacggcgac cacggcctga gcgccgcgga aggtgtccag acacgggtcg
    30361 tggccgaagg ccgcctttgc cgagcccgcg agccggtcga tgagccagct ctcgcggtag
    30421 tagttgtaca tcgcgaacgc gccctggtcg gtcaggtgct cgcgcaccga cgtcatcgcc
    30481 tcctcggtga acaggaagga ctcgagccgg atctgggagg cgccgctgac cagggtgagg
    30541 gagtcgggca gggcgaagag gatgagatcg tatttcttgt cggtgttctc gaggaaggcg
    30601 cgcccgtcgt tgacatgccg ggtgacgcgc gggtcctggt agggccggtc cacattgcgt
    30661 tcggccccga gctccatgat ccgcgggtcg atgtcgacgg cgtcgacctc ctgggcgcca
    30721 cgggagaggg cgatggcgac gtcggagccg gagccggcgc cgacgatcag caccttgccg
    30781 agggggttgt cgggcagccg gaggtatggg gtggcgtaga tccgctcgcc ctgctccact
    30841 ttccatttgg ccggggccat caactggtgt gggacgccgt tggcgttgat cagcaggaac
    30901 ccgctgctgg gggcgctggt gttttcggtc tggatcttgt agtagggcga ccagctcacc
    30961 cccgcggtga gggtctcggc gagcagcatc cagacgagca cgccggccgc gaggaggttg
    31021 aggatccgtt gccagagccc ccgggccagc gcgagacagg cgagcccagc gatgacgccc
    31081 cagacgatgg acggcgcgcg caggaacgcc aggacgctga aggccgtaat gccgcagagc
    31141 gagccgatca ggtcatagcg gtatgccgtc agcggcgcga gctggccgaa gcagcggccg
    31201 accagctcgg ccggaccggc caggatgatc gccacgagca cgaagacgac gggcagggcc
    31261 tgccatgcgg gcggcccgga gacgtgcagc gcggtgaagt agatgacatc ggatccttgt
    31321 cgttcgatcg tcaccgggaa ctcgcgcacg gcgacgacga gggccgccag gagcggcaac
    31381 gagaccggcc agatcgacca cttcttgttg ctgatcagga agccgcagcc gatgccgagg
    31441 aaggagccga gcagcacgaa gttggagaaa tagctcaggt gcacgacgtt cgcgccgagc
    31501 cagcggatca aggccagctc gacgaagagc atgagcgcgg ccgagagcac cagccggcgg
    31561 tcgatcgccc ctgacgtggt cgcaggctcg acccgcgagg aggacgacgt cgtagtcacg
    31621 ggcgagatgc tttcacacgg ccggcttggg gctagggcgg gcagtacgaa cttttccgcc
    31681 cgatgccact ttgtagcggt gcgcctcgcg acattcgcga tgcgtaccga tagaaacccg
    31741 cccgaagcgt caggcgccgg cgcaggtgcc gcacccgccc gtgccacagg cggcaccggc
    31801 ggctccggcg gcctcgtcac cgcagccgtc cccgtcggcg ccgccgcaac acccgccgcc
    31861 gcacccgccg gccgcgacgg gctcggggcg gttggcgcgg tccgcccagg cgtgccggat
    31921 ggcctcgagc aggacctgcg ggggctgagc cccggtcacg gcatacttct ggtcgagcac
    31981 gaaggtgggg accgcggtga tgccgatctg gccggcgacc gcctcgtcgg tgcgcacgtc
    32041 gatgccgtga tcgtcgctgc tcagcacccg ctcgatcgtc tcggcgtcga ggccgccctc
    32101 ggcggccagc cggcggaggg tgtcggcgtc gccgatcgcc gcgccctcgg tgtaccaccc
    32161 gcgcatgatc cgctcggtga ccgggccctc gaccccggcc tcgcgcgcgg cgtggatgag
    32221 gcggtgcgcg tcatacgaat tgctcggcac gactcggtcc cactggaagt ccagcccgac
    32281 ctccgctgcc cccgccgcca tctgttcgtg ctgagcgacc atctgctcgc gcgtcacgcc
    32341 gtacttctgg gcgatccgat cgacattgga tccggtgccg accgcggggg cgttgcggtc
    32401 cagctcgtag ctgtgccaga tgacgtcgac gtcgtcacga tgctcgaaat acgcgagggc
    32461 caggtcgagg tggcgccggc ccacgtggca gaacgggcag acgatgtcgg accagacgtc
    32521 gatacgcatg cgtttctcct atgtcaagcc gctgccgggg cggcaggggt ttgacggttg
    32581 t
//
LOCUS       DAOPJG010000014        31503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1518_length_31503_cov_7.813056, whole genome shotgun sequence.
ACCESSION   DAOPJG010000014 DAOPJG010000000
VERSION     DAOPJG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 31503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31503
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1518_length_31503_cov_7.813056"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PKU75_02790"
     CDS             complement(<1..297)
                     /locus_tag="PKU75_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79830.1"
                     /translation="MAISETQKNEFIDAYTKALLASWSNDEYAARLESDPRAAFAEVG
                     LDLPAGAEIVVTRGSANEATGDEPKARLDNQVALYEAGLESGRFEFHMPSTPQID"
     gene            complement(558..950)
                     /locus_tag="PKU75_02795"
     CDS             complement(558..950)
                     /locus_tag="PKU75_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79831.1"
                     /translation="MAISEKQKGDFVDAYTKALLASWSDEDYASRLESDPRTALAEVG
                     LFLPAGASVHVETGAPASTTVETVEPQARLQRQVDLYEAGFASGHFEFHMPATPQIDT
                     AELDIEDLASVSGGITCCSCCCPCCCCG"
     gene            complement(1043..1426)
                     /locus_tag="PKU75_02800"
     CDS             complement(1043..1426)
                     /locus_tag="PKU75_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79832.1"
                     /translation="MALNENQKNEFVDAYTKALLASWSSDEYAARLESDPRAALAEVG
                     LDLPADATIVVTRGAAEASSEGDAQGRLDNQVALYEAGLESGRFEFHMPSTPQIDTSE
                     LDVDELAGIAGGGIYCCCCPCCCCG"
     gene            complement(1670..4579)
                     /locus_tag="PKU75_02805"
     CDS             complement(1670..4579)
                     /locus_tag="PKU75_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF79833.1"
                     /translation="MANEIGPSGSWAADLPESFPVIWSQRDMHLVLGADDLFIVEAGE
                     VDLFAVQVRDDQPVGPWHGVGRAGPGDVIAGPVRGPRHRVLCRRVGDAELRIAPLNSV
                     HALLRDPETPEQVVSGLVSGIESTLRKITRGVQRELPPRDFRVLAGNAVNEFEDGDIV
                     RPIVDVLWVEVTQGRVFHGMDEREYGVGETVCVTRQDWLRVTQESRLRTWTTSQVHER
                     DGLWRALVSHWSRFLYFIDRLVERDELTSLEAVQRATDRDAEATMLVRRGNDTLLLRD
                     SLDVRGVTSGHVDAVTRVLAEMKADPAVPETISSRRDIGDYDQLSSGGWVRTRAIKLE
                     GRWWTKDMGPVAGYWGPDHLPSAFVFRDGGYVVQARWLDDPVRITSDNQFSAGRHVWA
                     IYPRLPLSVTSVKSLLGHSLRGRSGDFWLFGTMAALIGILGLLTPVLSGRVLGDYVAS
                     ANRSMIVQAGLAIVLSGLVGAAFAIVQNLTVLRLQGSVTASSQTGVWGRLLDLPVTFF
                     QRYSTGRLCTIVLSVKAAQEALSGVVVAATLGLVVVVANLILVFFFNTILALVALALA
                     VVPTVASWLLGKQVLTHERRRYEAEQQLTGMGYEVLSAMSKIRASAAEERAFLRWSDQ
                     QRVVQVHALASRRIQDRVTALNAVYPIFALAVLFIVATRLDPQPGLSTLLSFLTSATL
                     LINALLQFTGSVLTAAAIIPMLGSMEPILQAEPEAGVGKAHPGDLSGSVSLRGVSFRY
                     GLDGPLVLDDVSIDVNPGEFVAIVGPSGSGKSTVVRMLLGFAKPVSGAVLYDGQDLGE
                     LDLSAVRGQCGVVLQAGALMAGDMRANISAGGNYSDDDLWEAAEMAGLADDIRAMPMG
                     LATVVNESSQGLSGGQVQRLMIARALVNRPRFVIFDEATSALDNPTQRIVADATRALN
                     ATRIVVAHRMSTIIGADRIYVMDGGTIVQQGTHEELMADEDGLFAQLARRQEVDA"
     gene            complement(4579..6813)
                     /locus_tag="PKU75_02810"
     CDS             complement(4579..6813)
                     /locus_tag="PKU75_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="HPF79834.1"
                     /translation="MPTLKRGTRPRIERECAGLAPRRARVRRTPSVIQMEAAECGAAS
                     LSILLSHYGRFVPLEELRIACGISRDGSSAKNVVEAAQSYGLVGVGRRMEIQPMMALD
                     HPVIIFWAFQHFMVVNGFERRGGQTMVLINDPASGPRRMPLTEFDQGFTGIVLDLVPG
                     DDFITGGAPTKTLSMLRERISGTGRALTLALVASVLLVIPGVVVPVFTQYYIDRVFGT
                     EGTSLIPMLLGLTACAAATLILTRVQTHNLRLAESRSALVSAGRFLYRLLRLPLGFFL
                     QRRAGELGRRLSSNRQVAQTLTRDLVVTTVNLLLIVVYGGALLFQDLLLGTIAIGIAM
                     LNLIVLRAVMRRRVDSATALEAEESRLAVATLHTISAIETVKSCGTEHASFGRWSGSM
                     AKAMSESQKLGVPTALLTVMPPLLATVNSGIVILIGGLRVVDGAMTVGVLFAFQALLT
                     TFTRPLTQLTNQAGQIQELGAQLQRLRDAEKYELDLAFTTTDAGQTRVLTGNVTFDRV
                     DFTFGPLQAPLVVDFNLDLEPGMRVALVGASGSGKSTIAKLAAGLLQPSAGRVLYDGE
                     PRQAWSRQSMARSVAYVDQRSSLFEGTIRDNVCLWDDEISEATILRALSDAEIDGVIA
                     HRAGGLSSRIAEGGTNFSGGQRQRLELARALVSNPTLLILDEATSALDTSTERLVMDN
                     LRRRGCALMLIAHRLSTVRDADVILVIDQGRVVESGRHEDLMALDGHYRSLVESDASN
                     QEKQ"
     gene            complement(6817..7593)
                     /locus_tag="PKU75_02815"
     CDS             complement(6817..7593)
                     /locus_tag="PKU75_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPF79835.1"
                     /translation="MKFRFKALERQRHPDQLDSPLLLASPQGWVAVFTVLITTVMIGL
                     WAFLGSLPRSISVDGMVSYSGGVESVEAAVAGSVTRLAVAPGQVVSSGDVLATVRDRF
                     GNDTQVTSLADGRVIAIPSGLGSYVAAGDVVASVERIDNAEQPLSATLLVSGDLVPFV
                     HIDQQVNMSVPGVNPRAFGRLKGVVTAVSDYPATAPIEGSQESPVYAVDVQLLPDAAT
                     VSGYAWTSASGPPAELRSRTPVHAELSLGGVRPVSYLLGL"
     gene            complement(7590..8963)
                     /locus_tag="PKU75_02820"
     CDS             complement(7590..8963)
                     /locus_tag="PKU75_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HPF79836.1"
                     /translation="MLTMTEKLTLAVRPGVSLRAEPEGLVIDAPGWSQTLPVTEAGVS
                     ASLAGLTTDRSAEEIARDAVVASGSFGAAMTVQVVLARLQSLGVLEHRVVADGNLRAW
                     LRTAGAMPCAVSRMPDDGQVRTLSPLLIATLADGFVQLESGATHLKVGLTPELFGRLV
                     EEGHGSLPGPVADLLFSAGLLLTPDEAADRRVRQWDPSDAWFHRRVSESRGNDGYGGT
                     YHLAAEFDPLPYDRPAPGASIALPVPDESEPKGAPVIDAIEKRRSFRSFQAGKTTLAQ
                     LSQVLYRTVRARQTFPDDHGLDVVDRPYPSGGSIHELDTYLVINDVAGLDPGIYRYAP
                     LRHELDLISADPLARTRLSADAQIPLRAEVPAPIVMIISARFGRLMWKYQGMPYALLT
                     KHVGVLYQTTYLAGEAAGLGVCGIGGTSSTLFAQVTGEHPLDEGPVGMMVLGVADRTE
                     ADPWGRP"
     gene            complement(8993..11257)
                     /locus_tag="PKU75_02825"
     CDS             complement(8993..11257)
                     /locus_tag="PKU75_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HPF79837.1"
                     /translation="MNPLRFRDSLAVDVIDGTKLFLLNDETSVIVENPNTAAVAALIN
                     GERAIPQLMNELAGTLSPEQVFLEVAKLQRAGHVISRDSADDPATTYLESFGKATDDF
                     AGNQRTFTVGVLDLVDCALGESLSGALAAYPQLAVSTVDLSGDIDADLVVVVAGDYLD
                     PALREINKRFLDGQHWILTKPMGREVYAGPRILPGETACWESLADRIQANRQVERYVT
                     GKVGRSYPAVKPIGVSPGGVALATGILANEIFAVAAGMPGELAGSMRSIDVKTYEMRE
                     HTVVKNPQFSVDDPASLRRTSEVILGNYPALHLEDGGYRVCTPKETFERLEHHISHVC
                     GAVSRLVTLNVDEEGITHSYAAGHNFGMMGDNMDLLRNNMRGQSGGKGRTETQAKVSG
                     ICEAIERYSGVWTPDIPETLTTWNSLSTRAIHPRAYLNYSDAQYDSRDEWNRDPRNRL
                     QKIPMIFDEDRPVHFTAGRSLTTGEEVLVPSAMVWFGHPDLDQRDHLYTMTDSNGGAS
                     GNVIDEAVLQGLCEVYERDAVALWWFNRIARPGIDLASVQDPYVKQMQDFYATMDRDI
                     WVLDLSNDLSVPTFAAFSKRHHEVQDLMVGFGAHPDPHIAFFRALTELNQFLPFIRQR
                     DADGNTIYSVDDAATLEWCQTRTVESDPWVAPDPNVPLKTLESLERDLPTTLGDIVRF
                     CVDDLAAAGMETVVVNQTRPDIDLAVAKVLVPGMRHFWRRTGPGRIYDVPVALGWRDT
                     PLTEAEVNPIGVFF"
     gene            complement(11349..11534)
                     /locus_tag="PKU75_02830"
     CDS             complement(11349..11534)
                     /locus_tag="PKU75_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79838.1"
                     /translation="MNSNLKVFLAIFCFIWGIVGVVLAAVNLQAEPAGTTSAIAFAAV
                     GMVGFVAGWLLMRRPRY"
     gene            complement(11565..12365)
                     /locus_tag="PKU75_02835"
     CDS             complement(11565..12365)
                     /locus_tag="PKU75_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPF79839.1"
                     /translation="MTSSYSVGTGYARDIEFDRLDRQAALTFPRELQSLRAFGLTTDG
                     VVVDFGSGNGAVTERLRAELPDAVVYGADNDLSLLATVPPPTLAVKDGRIPLPDNAAD
                     DVLMRFVAQHLTPRQRMDVFAELWRVLRPGGRIHVIDVDDSDVGRSSGMVTRGLVEIF
                     GELQRIQGENGGDRLVVKKIPAELDNAGFEEVTPVAATVTTEDHPVSAFSVHMGPDRY
                     IPLVSAGVLSVEQLAVVGWSWERLRRDPNAFISINVHSVHARKPNAAV"
     gene            complement(12457..13704)
                     /locus_tag="PKU75_02840"
     CDS             complement(12457..13704)
                     /locus_tag="PKU75_02840"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HPF79840.1"
                     /translation="MALAILTVAVLLLWFVVKGAVGLIGGLFGGDDKAATVSAPVTKA
                     PAAAPTVAPDDTLPKVVRVTPTTTPAMGAAGPADKTTMVQKFHLASSAMAPKSIVASP
                     SGVLFAQNMMYKHSVSVFKSDGTMIKSIPDAVDLAEFGISGHPGLSQGAPVEVAFTPD
                     GKYAWVSNYSMYGKGFGPEGLDSCTAGDGTDTSYVYRIDVASLTIDKVVPVGAVPKYV
                     AITPDGKNVLVTNWCSWNLSVIDAAAAKETARIDLGGRYPRGIVAGPDNKTAYVALMG
                     SNKVVTVDLASKAVADFAKPGNGPRHIVASPDGKFLYVTNNSSGTVVKLDRATGATLA
                     TATVGSEPRSMAISSDGLAVYVVNYGEGSMTKIATATMTAMQTVKTDASPIGITYEPT
                     TKSVWVACYGGSLLAFDDAKLKG"
     gene            14131..15837
                     /locus_tag="PKU75_02845"
     CDS             14131..15837
                     /locus_tag="PKU75_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79841.1"
                     /translation="MKASVLHPPAPPTSTPRDFLLWGARLQWRTLAGGLVFGVAWMTS
                     LALVPWAIGRGLDAGIVADDRQALLRWALILVALSAVIGITGAIRHWFAVQNWLDGAF
                     RTIVVTDEAVTSRGQEIARQIPVGDVVSVSSTDMERIGGVYDTFARFCGAIVSYVVVA
                     VLLLRQSVTTGLVVLIGAPVLLGLLSLIIKPLQRRQAEQREESGRLTTLGTDTVAGLR
                     ILRGIGGEAEFVGRYAAQSQHVRAAGVRVAGFQAALDASQTLLPGLFVVIVVWTGARS
                     VLSGELSVGELVAFYGYATFMVFPLRTVTEMVEKSIRFRVAAGKVMSILRIQSDHPAG
                     KADQVVDPSADLSDPASGARIPGGRLTAVVSARPEDSAELAGRLGRLGTESTVALGEI
                     SLAELPIRQVRDLVLVNDPDSRLFSGTLREELSTGQAIDDDRLLKALDDAWARDVLLA
                     LPDGLDGHVEEKGRGFSGGQRQRLGLARALAADPHVLVLIEPTSAVDAHTEARIGASL
                     AQSRRGRTTVVATVSPLLLQHTDHVVLLESGRAIASGRHAHLVRDNAAYRHIVLRGGD
                     SE"
     gene            16002..17687
                     /locus_tag="PKU75_02850"
     CDS             16002..17687
                     /locus_tag="PKU75_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79842.1"
                     /translation="MLLSRHRGTLALVIGLHALAALASLALPLLVGRLIDDLRAGTTR
                     GQVDRFVLLLLATVIVQTALMWFARRAAFVFGEGLFADLRERFIDRASRLPLPVLERA
                     GTGDLVARTTNDVESLSWAARFALPSIFVALVTVVITVAATVVVSPLGSLAVLAVLPL
                     ALPATWWYLRRARTGYLWERAAWAQLNGVAAESADSARTIDALDLGDVRHGRLLESVR
                     RAYRAEYYTRWLRMWWFSPTEFSYVVPVAVALVWCGWLVADGAMTLGAATTVILYLRQ
                     LIDPLDELISWLDEIQVAATSLARIIGIENVPDDRRPSGATPADDAISARDVHFTYRA
                     DNPVLHGVSLDLIPGERLAIVGPSGAGKSTLGRLIAGIDAPGSGTVTVGAVPLMELSM
                     EDLRGEVALVTQEHHVFVGTLRDNLLLAKPRASDADLRTALASVDALEWATGLPTGLD
                     TEVGSGGHELTHPQSQQLALARLVLADPHTLVLDEATSLLDPRAARHLERSLAKVVDG
                     RTVVAIAHRLHTAHDADRVAVVEDGRISELGSHDELLAADGPYAALWRSWRAE"
     gene            complement(17799..19535)
                     /locus_tag="PKU75_02855"
     CDS             complement(17799..19535)
                     /locus_tag="PKU75_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HPF79843.1"
                     /translation="MLRAKAVRRGVAPATSGDHYDVVVIGSGFGGSVAALRAAEKGYR
                     VLVLEAGRRFSDEDFAKTNWDLRNYLWAPQLGCFGIQRIHKLPDVLVLAGAGVGGGSL
                     NYACTLYVPPSPFFQDRQWAGITDWQAELTPHYETATRMLAVVTNPCEGPVEAAMRDA
                     AESMGVGDTFRKTPVGIYFGTPGERVADPYFGGAGPDRTGCTQCGNCMVGCRVGAKNT
                     LMKNYLALAERLGVVIEPMRQVTELNPPAGEGGRWRVVTQRSGAKRPDRHTWTADQVV
                     LAAGTWGTQTLLHAMKDRGIMPRLSPRLGELTRTNSEALLGAQLAKYTPDADLSHGIA
                     ITSSFHPDHETHIENVRYGPGSNSMGLLKSLLVPGDTGRPRMLEMFRVMAKDPGQLLA
                     LNNYKWSQRAVIALVMQTRDNSVTVSGRRGRLGGRTLTSRQGHGEANPSWIPAGHAGT
                     RALAEALGKRVGVRAIPGGSWAELLGFPMTAHFLGGAVIGATAQDGVIDAYHRVWGYP
                     TLHITDGSAISANLGVNPSLTITAQAERAMSLWPKAGEADQRPPQGSAYVRLAPIAPA
                     LGDPAAHPAVVA"
     gene            complement(19547..21130)
                     /locus_tag="PKU75_02860"
     CDS             complement(19547..21130)
                     /locus_tag="PKU75_02860"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HPF79844.1"
                     /translation="MTATMTPAQATPGFALDPERVRTWAELVVCSREPEHFEVRTPMS
                     GEVIATLPLSTPEDAERAVTAARSSQKRWAATTFEARRDILLRFHDLLLDGQSELLDL
                     VQLESGKARSQAHEEVADTAIVARHYALTAEKTLRPRRRAGAYPVLTQSIESYLPKGV
                     VGIVSPWNYPLSLAITDAIPALMAGNAVVLRPDMQGSLTALAVVHLLHEAGLPDGALE
                     VVLGRGSTVGNAVLEHADYVCFTGSTATGRRIAQAAGERLVGCSMELGGKNSLYVAAD
                     ADLDRAVPGAVRACFASAGQLCISTERVIVHEKVYDAFVPRFAAAAAALRMGSALTYG
                     YDLGSLASAEQLEAVTRHVDDAVAKGATVLSGGRPRPDLGPFAYEATVLSGVTAEMAC
                     RNEETFGPVVSVYRVASDDEAVALANDTEYGLNASVWTRDVRRGRALAARIRTGTVNI
                     NEGYAAAWGSVAAPMGGMKASGIGRRHGDEGILRFTESQNVTAQRLLPIAPVFGMDDE
                     KFAKTLTSALRALKAARRA"
     assembly_gap    21324..21423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21524..22645)
                     /locus_tag="PKU75_02865"
     CDS             complement(21524..22645)
                     /locus_tag="PKU75_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HPF79845.1"
                     /translation="MAEIEIGRGKRGRRAYSFDEIAVVPSRRTRDPEDVSTDWQIDAY
                     RFEIPVLAAPMDSVMSPASAIAFGQAGGLPVLDLEGLWTRYEDPEPLLAEIATLEPAL
                     ATARMQEIYAEDIKPDLIGARLKEIRDAGVTVAGALSPQRTQQFWNSVVESHVDIFVI
                     RGTTVSAEHVSSRAEPLNLKRFIYELEVPVIVGGVASYTAALHLMRTGAAGVLVGFGG
                     GAAHTTRTALGIHAPMASAVADVAGARRDYLDESGGRYVHVIADGGMGVSGDIVKAMA
                     CGADAVMLGAALARATEAPGRGFHWGPEAHHRDLPRGELVEVGTAGSLQEILFGPSRS
                     ADGTTNLMGALRRAMATTGYLELKEFQRIDVVVSPHVRS"
     gene            complement(22760..24289)
                     /gene="guaB"
                     /locus_tag="PKU75_02870"
     CDS             complement(22760..24289)
                     /gene="guaB"
                     /locus_tag="PKU75_02870"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPF79846.1"
                     /translation="MTSEAPIDAIDHGGPFGLLGLTYDDVLLLPGETDVIPSEVDTTS
                     RLTRELSLRIPLVSAAMDTVTEARMAIAMARQGGIGVLHRNLSIEDQAYQVDLVKRTQ
                     TGIISNPITIGPDATLEDLDTVCGEYRVSGLPVVGVDNQLLGIITNRDLRFTPVAEWA
                     STKVADVMTPMPLFTAPVGIERDAAIALLRQNKRERLPIVDDEGRLAGLITVKDFVKS
                     EQFPNASKDADGRLLVAAAVGFFGDAWQRTTTLVDAGVDVLVADTANGHARALLEMVR
                     RIKSDPGMRHVQVIGGNVATREGAQALVDAGVDAVKVGVGPGSICTTRVVAGVGVPQI
                     TAIHQAAQACKPAGVPVIGDGGLQFSGDIAKALVAGADTVMIGGLFAGCEETPGETVY
                     INGKQFKAYRGMGSIGAMSSRGKKSYSKDRYFQADVESDDKIVPEGIEGQVAYRGPVA
                     QVTHQLIGGLHQSMFYVGARTVPELQARGRFVRITAAGLKESHPHDVQMVAEAPNYRG
                     R"
     gene            24490..24789
                     /locus_tag="PKU75_02875"
     CDS             24490..24789
                     /locus_tag="PKU75_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPF79847.1"
                     /translation="MPDLMTVPSPLATEWDWQHRGSCRSLSPEIFFHPEGERGSARRV
                     RDRAAKEVCAECPVLARCREHALRAREPYGVWGGMSEDERLAFYEAHGLPVRRAS"
     gene            complement(24803..26149)
                     /locus_tag="PKU75_02880"
     CDS             complement(24803..26149)
                     /locus_tag="PKU75_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79848.1"
                     /translation="MTARADRPSHLGGVRAWVTFGLGVVGYGTAVLGRTTLGVAGLSA
                     VEHFHARASIIATFVVLQLLVYAAMQIPAGVLLDRYGSRRMLTLGLATMGIGQIVMAF
                     ADSVTIAIPGRMILGAGDAFVFSSVIRLLPSWFPPRRVPIVTQFVGLSGQLGQIVSAL
                     PFAWLLDARGWRTAFLAVTLSALAAAALSWTFLRDRPPGIPAPVRPAVGMRANLRAAA
                     RHPGTLLGMWTHWTTCFAPMVFGMMWGFPYLTQGEGVSKPTASLLIGLLAVVGAIVGP
                     MLGVLVQRHPLRRSNLVLAMVAAGLVPWLLVLLWPGPAPLWLLVALCAGLAVGGPGSA
                     IGFDFARTFNPGHRLGAANAVVNLGGFTACVIATQLIGLVLDAAGDGPDYTLGQFRLA
                     MAVQLPLYAVGIVGMYLTRGRARRVLAAQGQVVPPWREALRREWRARTRGLNRPRV"
     gene            26243..26377
                     /locus_tag="PKU75_02885"
     CDS             26243..26377
                     /locus_tag="PKU75_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79849.1"
                     /translation="MRVRVATAGDVEALVEMKRQLIESFAAPDGVVCRLARAGGSRTA
                     "
     gene            26476..26766
                     /locus_tag="PKU75_02890"
     CDS             26476..26766
                     /locus_tag="PKU75_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79850.1"
                     /translation="MPTPRPPRIIVVTGVIMAGVEDGRVLETEHGVGYALVDLPPQVP
                     TGSRVHVRGWVDHERRTTLNQWPVLVVSRVEAMPEDVSADMTGPPPEGGGPA"
     gene            complement(26793..29771)
                     /locus_tag="PKU75_02895"
     CDS             complement(26793..29771)
                     /locus_tag="PKU75_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPF79851.1"
                     /translation="MKRIIAFAGVAATAVAFAGASAGGANATTPPSGKGVGGSYIVVM
                     KADPLLRSTKQSDLRTSGAQSKRAALKASHDTVLKGAGIAVSRKVNTYTNALNGFSVL
                     TDAKGAAALAKQPGVAAVLKDEMRQPTAIAAAVNEAKAAPSGVNDLQKFLGLDTGAYA
                     KGIKGNGVIVGVIDTGIWPEHPMLRDNGTFPAAPTLDESERSACAFGNQAWNPNDKPF
                     TCNNKLIGAREFLDVYKSQTGLEHDEFDSARDNEGHGTHTATTAAGNANVQSRIFGVN
                     KDIVSGIAPRAQIIAYKALGDAGGYGSDLTAAIDQAVADGVDVINYSIGGGAEVVSAD
                     TLAFLFAADAGVFAAVSAGNDGPGPETVGGPANVPWVTGVGATTFPRNFAGRVKLGDG
                     RVFKGTSVTKGTGVKPIIDGTHAGGTDSRYCVEGSLSAAKVRGKIVLCERGVNGRVAK
                     SAEVKRAGGVGMVLFNSTDGDTMFSDNFFVPTVMMDYTNGARIRRYANNTPGATARIT
                     NLGMDTLWHYSPTVTYFSSRGPTVPNSDTIKPDISAPGAQIMAGNTPMPSGGSQPAGQ
                     LWQAIAGTSMSSPVVAGSYALLKQAHPEWSAAAAKSALMTQSYAAVRKNDRVTPADPF
                     DTGSGMAKLGSPGAKSTSFQPGLVYDAGFYDYLGFLCDEGREAFANPDATCGGLANAG
                     YATTAENLNYPSIGMSAVPGTMTVKRTVTNVSSQAITATASVRAPQGYRVTVSPATIT
                     VAPGASKSFEVSVTNTGAPIGAWRFGSLTWQGSGYAVRSPIAVNATRIASPAEITGTG
                     TSGQATITSTVGVAGTYTATAHGLVPAEHHNDTVAQDPDQTYPSPDDGAGVDRIPYTL
                     NGVDHARWKLDVGGDVDLDIYLLDSSGKVVAQSTNGGTDEQIDLVRPADGTYTMIVHG
                     WAVGATPVAYDLRSWRVPATTGGSLSVVSGSPQIVAVGDKVDVVVGWSGLTAGVEYLG
                     SVTHRIGGTIEADTVVAVNP"
     gene            complement(30095..>31503)
                     /gene="groL"
                     /locus_tag="PKU75_02900"
     CDS             complement(30095..>31503)
                     /gene="groL"
                     /locus_tag="PKU75_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144865.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPF79852.1"
                     /translation="AQLAKEVATKTNDIAGDGTTTATVLAQAMVREGLRNVAAGASPA
                     SLKRGMDAAVEALNARLLENAREVDGKDDIASVAALSAQDDGIGSIIADAFDKVGKDG
                     VITVEESSTAETVLEFTEGMQFDKGYISPYMVSDPERMEAVLEDCYLLINQGKISTIA
                     DLLPVLEKVMKSGKPLAIIAEDVDGEALSTLVVNKLRGTFNAVAVKAPGFGDRRKAML
                     QDMAILTGGQVISEEVGLKLDQAGLQLLGQARRVVVTKDTTTIVDGAGSADEVAGRVN
                     TLKAEIDKTDSDWDREKLQERLAKLAGGVCVIKVGAHTEVELKEKKHRIEDAISATRA
                     AIEEGIVAGGGTALVHALPALDDLGLTGDELTGANIVRKAAAEPLRWIAENAGMEGYV
                     AVSKVGELPIGQGLNAATGEYVDLAGVGVVDPVKVTRSALRNAASIASMVLTTDALVV
                     EKKEEEPAAPAAGHGHGH"
BASE COUNT         4768 a        10549 c        11188 g         4898 t
ORIGIN      
        1 gtcgatctgg ggggtcgacg gcatgtggaa ctcgaaacgc ccggactcca gcccggcctc
       61 gtagagtgcc acctggttgt ccagccgcgc cttgggctca tccccggtgg cctcgttcgc
      121 cgagccgcgc gtgacgacga tctctgcccc cgcggggagg tccaggccga cctcggcgaa
      181 ggcggcccgg gggtcgctct cgaggcgggc ggcatactcg tcgttggacc agctcgcgag
      241 gagggccttg gtgtaggcgt cgatgaactc gttcttctgg gtctcgctga tcgccatgat
      301 tgtggccttt ctctgattcg atagggggcc cctgcattca ggcctgcccc actgtcgggc
      361 ggcgacccgt acgcggggga agagcgcaac gggcttaacg cgcacgtagc gcccgcggcg
      421 gccgttcgta tgccgctgca ccgcatacgc agcgcgtccc cgcgccctcg ggtccacagt
      481 cgacagcgca cgactcgccg taatggcact tccattacgg cgagtcgtgc gctttcgacg
      541 ggccccagga gggatggtca gccgcagcag cagcaggggc agcagcacga acagcacgtg
      601 atgccgccgg aaaccgacgc caggtcctcg atgtcgagct cggcggtgtc gatctgcggt
      661 gtcgccggca tgtggaactc gaagtgaccg gaggcgaacc cggcctcgta caggtcgacc
      721 tgtcgttgca ggcgagcctg gggttcgacg gtttcgaccg tcgtgcttgc cggcgcgccg
      781 gtttcgacgt gaacgcttgc gccggcgggc aggaacagac ccacctcggc cagcgcggtc
      841 cgcgggtcac tctcgagtcg agaggcatag tcctcgtccg accaactggc gagcaacgcc
      901 ttggtgtagg cgtcgacgaa gtcgcccttc tgcttctcag aaattgccat gcggtccttc
      961 tcctttcagc ggttcggttg cgtcgcggga aaagcgacgt ggccgggtcc agcggacccg
     1021 gccacgtcgc tctgggcgag ggtcagccgc agcagcagca ggggcagcag cagcagtaga
     1081 tgccaccacc ggcgatgccg gcgagctcgt cgacgtcgag ctcggaggtg tcgatctggg
     1141 gggtggacgg catgtggaat tcgaaccgcc cggactccaa gccggcctcg tacagggcga
     1201 cctggttgtc cagtcggccc tgcgcgtctc cttcgctgga cgcctccgcc gcgccgcggg
     1261 tcacaacaat cgtcgcgtcg gcgggcaggt ccaggccgac ctcggccagc gccgcgcgcg
     1321 ggtcgctctc gaggcgggcg gcgtactcgt cgctcgacca gctggccagg agggccttgg
     1381 tgtacgcgtc gacgaactcg ttcttctggt tctcattgag cgccatggct tgtgcctttc
     1441 ttccttatgt gggtgtcggc cggggctgcc ctgtgcctcc ctggcactgc ccatcctgtg
     1501 cggcgatccc ccgcgcaccg gaagccttct tgacgccgaa tacccaccta ggacaacact
     1561 tttcgcttcg tttggtaccc agcccatacg caaggggtac gtagccggca gccgcggaca
     1621 cgaatgcggg gcctccggtc cggaggcccc gcggcatctg tgcgaagcgt caggcgtcga
     1681 cctcctgacg gcgggcgagt tgggcgaaca gaccgtcctc gtcggccatc aactcctcgt
     1741 gcgtgccctg ctgcacgatc gtcccgccat ccatgacgta gatgcggtcg gcaccgatga
     1801 tggtggacat ccgatgggcc acaacgattc tcgtggcgtt gagggcgcgc gtcgcatcgg
     1861 cgacgatccg ctgtgtcgga ttgtccaggg cgctggtggc ttcgtcgaag atgacgaatc
     1921 ggggacgatt gacgagcgct cgggcgatca tgagtcgctg cacctggccg cctgacaggc
     1981 cctgagagga ttcgttcacg accgttgcca agcccatcgg catggcccgg atgtcgtccg
     2041 cgagaccagc catctcggcg gcctcccaga gatcgtcgtc cgaatagttg cctcccgcag
     2101 agatattcgc tcgcatgtcc ccggccatca gggccccggc ctgcagcacc acgccgcatt
     2161 gaccgcgcac cgcggacagg tcgagttcgc cgaggtcctg gccgtcgtag agcacggccc
     2221 cactcaccgg tttggcgaac cccagcagca tccggacgac cgtggacttg ccgcttccgg
     2281 acggcccgac gatcgcgacg aactcgccgg ggttgacgtc gatggagaca tcgtcgagca
     2341 cgagcggccc gtccaggccg tagcggaagg agacgccccg caggctgacg ctgccggaca
     2401 gatcgcccgg gtgggccttg ccgacgccgg cctccggttc ggcctggagg atgggctcca
     2461 tcgatccgag catcgggatg atcgccgcgg ccgtcaggac actcccggtg aactgcagga
     2521 gcgcattgat cagcaaggtg gccgaggtga ggaatgacag cagggtcgat aggccgggtt
     2581 gcggatcgag gcgcgtcgcg acgatgaaca ggacggccag ggcgaagatc ggatagaccg
     2641 cgttgagcgc ggtgacccgg tcctggatac gccggctcgc cagcgcgtgg acctgcacga
     2701 cccgctgctg atcggaccag cgcaggaagg cgcgttcctc cgcggcggaa gcgcggatct
     2761 tcgacatcgc cgacaacacc tcatacccca tgcccgtcag ctgctgctct gcctcgtagc
     2821 gccgccgctc gtgggtgagc acctgcttgc cgaggagcca cgacgcgacg gtcgggacga
     2881 ccgccagcgc cagcgcgacg agggccagga tcgtgttgaa gaagaagacg aggatgaggt
     2941 tcgccacgac gacgaccagg ccgagggtgg cggcgacgac gacccccgag agcgcctctt
     3001 gggcggcctt gacactcaag acgatggtgc agagccggcc ggtcgaatag cgctggaaga
     3061 aggtcacagg caggtcgagc agccggcccc agacgcctgt ctgcgaactc gcggtgacgg
     3121 atccctgcag gcgcaacacg gtcaggttct ggacgatcgc gaacgccgcg ccgaccagac
     3181 cgctgagcac gatcgccaat cccgcttgca cgatcatcga ccggttcgcg gaggcgacat
     3241 agtcgcccaa gactcgtccg ctgagaaccg gggtcagcag accgaggatc ccgatgaggg
     3301 cggccatggt cccgaacagc cagaagtctc cggagcgtcc gcgcaggctg tggccgagca
     3361 agctcttgac cgacgtaacg gagagcggca ggcgaggata gatggcccac acgtggcgcc
     3421 cggcgctgaa ctggttgtcc gacgtgatcc gcacgggatc gtcgagccag cgggcctgga
     3481 cgacgtaacc gccgtcgcgg aagacgaaag cactcggcag atggtccggc ccccagtagc
     3541 cggcaaccgg ccccatgtcc ttggtccacc agcggccctc gagcttgatc gcccgcgtcc
     3601 ggacccagcc gccggaggac agctggtcgt agtcgccgat atcgcgacgc gacgagatgg
     3661 tctccggcac agccggatcc gccttcatct ccgccaggac acgggtgacg gcgtcgacat
     3721 ggccggaagt gacaccgcgc acgtcgaggc tgtcgcgcag caggagggtg tcattgccgc
     3781 ggcgcacgag catcgtggcc tcggcgtcgc gatccgtcgc gcgctgcacc gcttcgaggg
     3841 aagtcagttc gtcgcgctcg acgaggcggt cgatgaaata gaggaagcgg gaccagtggg
     3901 agaccaacgc ccgccagagg ccgtcgcgtt cgtgcacctg agacgtggtc caggtccgca
     3961 gccggctttc ctgggtcacc cgcagccagt cctgccgggt gacacacacc gtctcgccga
     4021 cgccgtactc gcgttcgtcc atgccgtgga agacccttcc ctgggtgact tccacccaca
     4081 ggacatcgac gatgggacgc acgatgtcgc cgtcctcgaa ttcgttgacg gcattcccgg
     4141 cgaggacccg gaagtcccgg ggtggcagtt cgcgctgcac gccgcgcgtg atcttgcgca
     4201 gggtcgattc gatgccgctg acgaggccgg agaccacctg ctcgggtgtc tccgggtcgc
     4261 gcagcagcgc gtgcaccgaa ttcagcggcg cgatccgcaa ctcggcatcc ccgacccgac
     4321 gacacagcac ccggtgccgg gggccgcgca ccgggccggc gatgacgtca cccgggccgg
     4381 cccggcccac gccatgccag ggccccaccg gctgatcgtc gcggacctgc acggcgaaga
     4441 gatcgacctc accggcctcg acgatgaaca ggtcgtcggc accgaggacc agatgcatgt
     4501 cccgctgcga ccagatcacc gggaagctct ccggaaggtc cgcagcccat gaccccgacg
     4561 ggccgatctc gttggccatc actgcttctc ctggttagag gcgtcggact cgacgagcga
     4621 tcgatagtgc ccgtcgaggg ccatgaggtc ctcatgccgg cccgactcca cgacgcgtcc
     4681 ctggtcgatc acgaggatca cgtcggcatc ccgcaccgtc gagagacggt gcgcgatgag
     4741 catcagggca cacccgcggc ggcgcagatt gtccatgacg agacgttcgg tcgaggtgtc
     4801 gagggcactg gtcgcctcgt cgaggatgag caacgtcgga ttgctcacca gggcgcgcgc
     4861 cagctcgagc cgttgccgtt gcccgccaga gaagttcgtg ccgccctcgg cgatccggga
     4921 actgaggcca ccggcccggt gcgcgatcac cccgtcgatc tcggcgtccg acaacgctcg
     4981 caggatcgtc gcctcactga tctcgtcgtc ccacagacac acgttgtcgc ggatcgtccc
     5041 ctcgaagaga ctgctgcgct gatcgacata cgccaccgac cgggccatgg actgtcggct
     5101 ccaggcctgc cgcggctccc cgtcatacag caccctcccg gcactcggct gcagcagccc
     5161 ggccgccagt ttggcgatcg tgctcttgcc cgacccggac gcgccgacga gcgccacgcg
     5221 catccccggc tcgagatcga ggttgaagtc gacgacgagc ggcgcctgca gcggcccgaa
     5281 ggtgaagtcg acacggtcga aggtgacatt gccggtcagc acccgggttt gccccgcatc
     5341 agtggtggtg aaggccaggt cgagctcgta cttctccgcg tcgcgcagcc gctgcagttg
     5401 ggcgcccaac tcctggatct gcccagcctg gttggtcagc tgggtcagcg ggcgggtgaa
     5461 ggtggtcagc agcgcctgga aggcgaacag gacgcccacc gtcatcgcgc cgtcgacaac
     5521 ccgcagcccg ccgatcagga tgacgatccc ggagttgacg gttgccagca gcggcggcat
     5581 gaccgtgagc aaggcggtgg gcacgccgag cttctgcgac tcgctcatcg ccttcgccat
     5641 cgaccccgac caccggccaa atgaggcgtg ctcggtgccg caggacttca ccgtctcgat
     5701 cgcggagatg gtatgcagcg tggccacggc gagccgggat tcctcggctt cgagtgcggt
     5761 cgcgctgtcg acgcgacgac gcatcaccgc gcgcaacacg atgaggttga gcatggcgat
     5821 gccgatggcg atcgtgccca acaggaggtc ctggaagagc agcgccccgc catagacgac
     5881 gatcagcagc aggttgacgg tcgtgacgac caggtcgcgg gtcagggtct gggcgacctg
     5941 cctgttgctc gacaggcggc ggcccagttc gcctgcgcgg cgttgcagga agaagccgag
     6001 cggcagccgc agcagccgat acaggaagcg gccggcggag acgagcgcgc tgcgtgattc
     6061 ggccaggcgc aggttgtggg tctgcacgcg agtcaggatc aatgtcgctg cggcgcaggc
     6121 ggtcaggccg agcagcatgg ggatcaggga ggtcccctcg gtgccgaaga cccggtcgat
     6181 gtagtactgc gtgaagaccg gcacgaccac gccgggaatc acgagcagga cactcgcgac
     6241 gagagcgagc gttagagccc gcccggtgcc ggaaatacgc tcccggagca tgctcaacgt
     6301 cttggtaggg gcaccgccgg taatgaagtc gtcgccgggc accaggtcca ggacgatgcc
     6361 ggtgaatccc tggtcgaact ccgtgagcgg catccgccgc ggaccggagg ccgggtcgtt
     6421 gatgaggacc atcgtctgcc cgccgcggcg ttcgaacccg ttgacgacca tgaagtgctg
     6481 gaaggcccag aagatgatga ccggatgatc cagcgccatc atgggctgga tctccatgcg
     6541 ccggccgacc ccgaccaggc cgtagctctg agccgcctcg acgacattct tggcggagga
     6601 gccgtcacgg gagatgccgc acgcgatccg cagttcttcc agcgggacga accggccgta
     6661 gtgcgacagc aggatgctca acgacgccgc cccgcactcg gcggcctcca tctggatgac
     6721 cgacggagtc cggcggaccc gggcccgccg cggcgccagc ccggcacatt cgcgttcgat
     6781 ccgcgggcgg gtgccgcgct tgagggtggg catggctcac agccccagca gatacgagac
     6841 cggacgcaca ccaccgagtg acaattcggc gtgcaccggt gtgcgagaac gtaactcagc
     6901 gggcgggccg gaggcgctgg tccaggcgta gccgctgacc gtggcggcgt ccggcagcag
     6961 ctggacgtcg acggcataca ccggcgactc ctgcgacccc tcgatgggag ccgtcgccgg
     7021 atagtcgctg accgcggtca cgaccccctt gaggcgaccg aacgcgcgcg ggttgactcc
     7081 gggcacggac atgttcacct gctgatcgat gtggacgaag ggcacgaggt cgccgctgac
     7141 gaggagggtg gccgacaacg gctgctcggc gttgtcgatg cgctcgacgc tcgcgacgac
     7201 gtccccggct gcgacataac tgccgagccc gctcgggatc gcgatcacgc ggccgtcggc
     7261 gagggaggtg acctgggtgt cattgccgaa ccggtcccgg acggtggcga gcacgtcgcc
     7321 gctgctcacc acctggccgg gcgcgaccgc caggcgggtg acgctgccgg cgacggccgc
     7381 ctcgaccgac tccaccccgc cggaatagga gaccatgccg tcgacggaga tcgaccgggg
     7441 cagcgagccg aggaaggccc acaacccgat catgaccgtc gtgatcagga cggtgaacac
     7501 ggcgacccag ccttgcggag acgcgagcag cagcggtgag tcgagctggt ctggatgtcg
     7561 ttgccgctca agggctttga accggaactt catggccggc cccaggggtc cgcttcggtg
     7621 cggtccgcca ccccgagcac catcatgccg accggtccct catccagcgg gtgctcaccg
     7681 gtgacctggg cgaagagcgt cgacgacgtg ccgccgatgc cgcatacgcc caggcccgcc
     7741 gcctcaccgg cgagataggt cgtctggtag agcaccccga catgcttggt cagcagggca
     7801 tacggcatac cctggtattt ccacatgagt cgtccgaagc gggccgagat gatcatgacg
     7861 atcggggccg ggacctcagc ccgcaacggg atctgggcgt cggcgctcag ccgcgtgcgc
     7921 gccaacgggt cggcgctgat caggtccaac tcgtgccgca gcggggcata ccggtagatg
     7981 cccgggtcga gtcccgcgac gtcgttgatg acgagatagg tgtcgagttc gtggatcgaa
     8041 ccgccgctgg ggtaggggcg atcgacgacg tcgagcccgt gatcgtcggg gaaggtctgg
     8101 cgggcgcgca ccgtgcgata gagcacctgc gacagctggg cgagcgtggt cttgccggcc
     8161 tggaagctgc ggaagctgcg gcgcttctcg atggcgtcga tgaccggcgc acccttgggc
     8221 tcgctctcgt cggggacggg cagggcgatg ctggccccgg gggcgggccg gtcgtatggc
     8281 agtgggtcga actccgccgc gaggtgatag gtcccgccgt aaccgtcgtt gccccgggat
     8341 tcggagaccc gccggtggaa ccacgcgtca ctcggatccc attgccgcac acgacgatcg
     8401 gcggcctcgt caggcgtgag aaggagaccc gcggagaaga gcagatcggc cacggggccc
     8461 ggcaggcttc cgtgtccctc ctcgacgagg cgtccgaaga gctccggagt cagcccgacc
     8521 ttcaggtgcg tcgcgcccga ctccagctgg acgaagccgt cggcgagcgt ggcgatcaac
     8581 agcgggctga gggtgcgcac ctgcccgtcg tccggcatac ggctcacggc gcagggcatc
     8641 gccccggccg tgcgcagcca cgcgcggagg tttccgtcgg cgaccacccg atgctccagg
     8701 acgcccaagg actgcagccg ggccagcacg acctggacgg tcatcgcggc gccgaaggac
     8761 ccggaggcga cgacggcgtc gcgggcgatc tcctccgccg aacggtcggt cgtcagtccc
     8821 gccaggcttg cgctcacccc ggcctcggtg acgggaaggg tctggctcca cccgggggcg
     8881 tcgatgacga gcccctccgg ctcggcccgc agcgacaccc cgggccgcac ggccagcgtg
     8941 agcttctcgg tcatggtcag catggcaact cctccttcgg gtcggatgca ggtcagaaga
     9001 agacgccgat ggggttgacc tcggcctcgg tcagcggagt gtcccgccag ccgagagcga
     9061 ccgggacgtc atagatgcgt cccgggcccg tgcggcgcca gaagtggcgc atgcccggca
     9121 cgaggacctt cgcgacggcg aggtcgatgt ccggccgggt ctggttgacc acgacggtct
     9181 ccatgccggc ggcggcgagg tcgtcgacgc agaagcgcac gatgtcgccc agggtagtcg
     9241 ggaggtcccg ctcgagggac tcgagggtct tcaggggcac attggggtcc ggggccaccc
     9301 aggggtcgga ctccacggta cgggtctggc accactccag ggtggccgcg tcgtcgaccg
     9361 agtagatcgt gttgccgtcg gcgtcccgtt ggcggatgaa cggcaggaac tggttgagct
     9421 cggtcagggc ccggaagaac gcgatgtgcg ggtcggggtg ggcgccgaag ccgaccatga
     9481 ggtcctgcac ctcgtggtgc cgcttggaga aggcggcgaa cgtcgggacg ctgaggtcgt
     9541 tggaaaggtc gaggacccag atgtcgcggt ccatggtcgc gtagaagtcc tgcatctgct
     9601 tgacgtaggg gtcctgcacg ctcgcgaggt cgataccggg acgggcgatc cggttgaacc
     9661 accacagcgc caccgcgtcg cgctcgtaga cttcgcacaa gccctgcagg acggcctcgt
     9721 cgatgacatt gccggaggct ccgccgttgc tgtcggtcat cgtgtagagg tggtcgcgct
     9781 ggtccaggtc ggggtgaccg aaccacacca tcgccgaggg gacgaggacc tcctcgccgg
     9841 tggtcagcga ccggccggcg gtgaagtgca ccgggcggtc ctcgtcgaaa atcatcggga
     9901 tcttctgcaa ccggttgcgc gggtccctgt tccactcgtc gcggctgtcg tattgcgcgt
     9961 cggagtagtt cagatacgcc cgggggtgga ttgccctggt gctcaacgag ttccacgtcg
    10021 tcagggtctc ggggatgtcc ggggtccaca cgccggagta gcgctcgatg gcctcgcaga
    10081 tgcccgacac cttggcctgt gtctcggtgc ggcccttgcc cccggactgg ccgcgcatat
    10141 tgttgcgcag caggtccatg ttgtcgccca tcatcccgaa gttgtgaccg gcggcgtagg
    10201 agtgggtgat cccttcctcg tcgacgttga gggtcaccag gcgggagacg gcgccgcaca
    10261 cgtggctgat gtggtgctcc agacgctcga acgtctcctt cggtgtgcag acccggtagc
    10321 cgccgtcctc gaggtgcaag gcgggatagt tgccgaggat gacctcgctg gtgcgccgca
    10381 ggctcgcggg gtcgtcgacg ctgaactgcg ggttcttgac gacggtgtgt tcgcgcatct
    10441 cgtaggtctt gacgtcgatc gatcgcatcg agcccgcgag ttcgcccggc atcccggcgg
    10501 ccacggcgaa gatctcgttg gccaggatgc cggtggccag ggcgacgccg ccgggtgaga
    10561 caccgatggg cttgacggcc ggatagctgc gccccacctt gcccgtgaca taacgctcca
    10621 cctgccggtt ggcctggatg cggtcggcga gcgactccca gcaggcggtc tcgcccggga
    10681 ggatgcgcgg accggcgtag acctcccggc ccatcggctt ggtcaggatc cagtgctggc
    10741 cgtcgaggaa ccgcttgttg atctcccgca gggccgggtc gaggtagtca ccggccacga
    10801 ccacgacgag atccgcgtcg atatcgcccg acaggtcgac cgtcgacaca gccagctggg
    10861 ggtatgccgc gagggccccc gaaaggctct cgcccagagc acaatcgaca aggtcgagca
    10921 cgccgacggt gaaggtgcgc tggttgccgg cgaagtcgtc cgtcgccttg ccgaagctct
    10981 cgagataggt cgtggccgga tcgtccgcgc tgtcccggga gatgacatgt ccggcccgct
    11041 gcagcttcgc gacctcgagg aagacctgct cggggcttaa ggtcccggcg agttcgttca
    11101 tcagttgcgg gatggcgcgt tcgccgttga tcagggcggc cacggcggcc gtgttcgggt
    11161 tctcgacgat cacgctcgtc tcgtcgttga gcaggaagag cttcgtgcca tcgatgacgt
    11221 cgacggcaag gctgtcacga aaacgcaagg gattcacgcc tggtcctttc acggccacgg
    11281 tgctgggaag tgttgtgcgg gaagcgaagc tggtcccacg cgctgcgtgg gcggaccctg
    11341 agcggggcct agtagcgggg gcggcgcatg agcagccagc cggcgacgaa gccgaccatg
    11401 ccgacagcgg cgaaggcgat ggccgaggtg gtgccggccg gttcggcctg gaggttcacc
    11461 gcggcgagaa cgacgccgac gatgccccag atgaagcaga agatggcgag gaagactttc
    11521 agattgctgt tcaccggagg gtccctttct gctggcgcgg cacgtcagac cgcggcgttg
    11581 ggcttgcggg cgtggacact gtggacattg atggagatga aggcgttggg gtcgcggcgt
    11641 agtcgttccc acgaccagcc cacgacggcg agctgttcga cggagagcac tccggcggag
    11701 acgaggggga tatagcggtc cggtcccatg tgcacgctga acgccgagac ggggtggtcc
    11761 tccgtcgtca ccgtggccgc gaccggggtg acctcctcga agccggcgtt gtcgagttcg
    11821 gccgggatct tcttgacgac cagccggtcg ccgccgttct cgccctggat ccgttgcagt
    11881 tcgccgaaga tctcgacgag gccgcgcgtg accatgcccg agctgcgacc gacgtcgctg
    11941 tcgtcgacgt cgatgacgtg gatccggccg ccgggacgca gcacgcgcca cagttcggcg
    12001 aagacgtcca tccgctgccg cggcgtgaga tgttgcgcga caaagcgcat gagcacgtcg
    12061 tcggcagcgt tgtccggcaa cgggatccgg ccgtccttca cggcgagcgt cggcggcggc
    12121 acggtggcca gcaggctgag gtcgttatcg gcgccataga cgacggcatc cggcaactcg
    12181 gcacgcaggc gctcggtgac ggcgccgttg ccgctgccga agtcgacgac cacaccgtcg
    12241 gtggtgagcc cgaacgccct gaggctctgc agttctcgag ggaaggtgag cgccgcctga
    12301 cgatccagtc ggtcgaactc gatgtcccgt gcgtatccgg taccgacgct gtagctggaa
    12361 gtcatggccc tacccaaatg ggctcaggcc ttgcggctca acgcctcccg gggctaatac
    12421 gtactccccc gcgggcgggt cagcatcgtc tcggtatcag cccttgagct tcgcgtcgtc
    12481 gaaggcgagc aggctgccgc cgtagcaggc gacccagacc gacttggtgg tcggctcgta
    12541 ggtgataccg atcggcgagg cgtcggtctt aacggtctgc atcgccgtca tcgtggcggt
    12601 ggcgatcttg gtcatcgagc cctcgccgta gttcacgaca tagacggcga gaccgtcgct
    12661 gctgatcgcc atgctccgcg gctcggagcc gaccgtggcg gtggcgaggg tggcgccggt
    12721 ggcgcgatcg agcttgacga cggtgcccga ggagttgttg gtcacataga ggaacttgcc
    12781 gtcggggctg gcgacgatgt gccggggccc gttgcccggt ttggcgaagt cggcgaccgc
    12841 cttgctcgcg aggtcgacgg tgacgacctt gttggacccc atcagcgcga catacgcggt
    12901 cttgttgtcc ggtccggcga cgatcccgcg gggatatctg ccgcccaagt cgatccgtgc
    12961 cgtctccttg gcggcggcgg cgtcgatcac cgagaggttc caactgcacc agttggtcac
    13021 caggacgttc ttgccgtccg gggtgatcgc gacatacttc ggcacggccc cgacggggac
    13081 gaccttgtcg atggtgagcg aggcgacgtc gatgcgatag acgtatgagg tgtcggtgcc
    13141 gtcgccggct gtgcacgagt cgagcccttc agggccaaaa cccttgccgt acatagaata
    13201 attggacacc caggcgtact tgccgtcggg cgtgaaagcg acctcgacgg gggcgccctg
    13261 cgagagaccg gggtgcccgc tgatgccgaa ctcggcgagg tccaccgcat ccgggatgga
    13321 cttgatcatc gtgccgtcgg acttgaacac cgacaccgag tgcttgtaca tcatgttctg
    13381 cgcgaacagc acgccgctcg gactggcgac gatcgacttc ggcgccatgg ctgacgaagc
    13441 caggtggaac ttctgcacca tggtcgtctt gtcggccgga ccggcggccc ccatcgccgg
    13501 cgtcgtggtc ggtgtcaccc gcacgacttt gggcagagtg tcgtcgggtg cgaccgtcgg
    13561 cgcggcggcc ggggctttcg tgaccggcgc cgagaccgtg gccgccttgt cgtcacctcc
    13621 gaacagcccg ccgatcaggc cgacggcccc cttgacgacg aaccacagga ggagcaccgc
    13681 gaccgtcagg atcgccagcg ccatggcccg ccgcagggca aagtttggct tcttgcgcgg
    13741 cggccccggc cgtggtccgc gaggccttcc cggcccgccc ggtcccccgg gcccaccgcc
    13801 ccgccggggt tgcacgcgtg ccggcatcgc cgcgggcgcc tcgcgcgggg gcagggtcgg
    13861 cacggtgaag gtggcgcgct cgccgccatg ggcgtgagga tcgttgtggg cgctaggttc
    13921 gttgtgggcg cgaggttcgt tgtgagcgcg aggctcgttg taggcgcgcg cctgcggcgc
    13981 gtcctcggcc gctgggtact cctcctcgcg gcgcgccatc gtcgtcacga ggccaggcta
    14041 agcgccgaac ccccggccgc cgtggcgcca caccgagcac cgaaaatcgc tcgtgtcccc
    14101 agcggggggt ccgtagcctg gatcaccgtc atgaaagcgt cagttctcca cccaccggcg
    14161 ccgcccacct ccacaccgcg cgatttcctg ctatggggtg cgcggctgca gtggcgcacc
    14221 ctcgcgggcg gcttggtctt cggggtggcg tggatgacct cgttggcgct ggtgccgtgg
    14281 gcgatcgggc gcggtctcga cgccggcatc gtcgccgacg accggcaggc gctgctgcgg
    14341 tgggccctga ttctcgtggc gctgagcgcg gtcatcggga tcaccggcgc gatccggcac
    14401 tggttcgccg tgcagaactg gctcgacggc gccttccgca cgatcgtcgt gaccgacgag
    14461 gccgtcacga gccgcgggca ggagatcgcg cggcagatcc cggtcgggga tgtcgtcagc
    14521 gtgtcgtcga cggacatgga gcggatcggc ggcgtctatg acaccttcgc ccgcttctgc
    14581 ggcgccatcg tgtcgtatgt cgtggtcgcc gtcctgctgt tgcgccagtc ggtcacgacg
    14641 ggactcgtcg tcctcatcgg cgcgccggtg ctgctggggc tgctgtcgtt gatcatcaag
    14701 ccgctgcagc ggcgccaggc cgaacagcgt gaagagtcgg gccggctgac gaccctgggc
    14761 accgacaccg ttgcgggact tcgcattctg cgcgggatcg gcggcgaggc ggagttcgtc
    14821 ggacggtatg ccgcgcaatc ccagcacgtg cgcgcggcgg gcgtgcgcgt ggccggcttc
    14881 caggccgctc tcgatgcgag ccagacgctg ttacccgggc tgttcgtcgt catcgtcgtg
    14941 tggaccgggg cccggtcggt gctctccggg gaattgagcg tcggcgaact cgtcgccttc
    15001 tacggctacg cgacattcat ggtcttcccg ctgcgcaccg tcacggagat ggtcgagaag
    15061 tccatccgat tccgcgtcgc cgcgggcaag gtgatgtcga tcctgcgcat tcagagcgac
    15121 catcccgcgg ggaaggcgga ccaggtcgtg gacccgtccg ccgacctgtc cgacccggcc
    15181 agtggcgccc gcatccccgg cggacggctg accgcggtgg tctcggcacg gccggaggac
    15241 tcggccgagc tggccggtcg cctcggccgg ctgggcaccg aatcaacggt cgcgctcggt
    15301 gagatttcct tggcggagtt gccgatccgt caggtgcgcg acctggtgct ggtcaacgat
    15361 ccggacagcc gccttttcag cggcaccctg cgtgaggagt tgtccacggg ccaggccatc
    15421 gacgacgacc ggctgctgaa ggcgctcgac gacgcctggg ctcgtgacgt cctgctggcc
    15481 ctgcccgacg gcttggacgg ccacgtggag gagaagggca ggggtttctc cggcgggcag
    15541 cgccaacgcc tgggcctggc cagggcgctc gcggcggacc cgcatgtgct cgtgctcatc
    15601 gaaccgacga gcgctgtcga cgctcacacc gaggcccgga tcggcgcctc cctggcgcaa
    15661 tcccggcgcg gccggacgac cgtcgtcgcc accgtcagcc cactactgct gcagcacacc
    15721 gaccacgtcg tgttgctcga gtccgggcgg gcgatcgcgt ccggccggca cgcccacctg
    15781 gtgcgcgaca acgcggcata ccggcacatc gtcctgcgag gaggtgacag cgagtgacca
    15841 ccaccgaaac ccgcacgcgg accggcacgc ccccggctcc cccgcggcaa cctgcgcggc
    15901 agagttggct gcgccgcgcc gaacccactg cggcgcagat cgatccgttt cgtcgctcgc
    15961 tcccggtcgc cgagccgagc caggtgtggg cgcacacgaa gatgctgctg tcccgtcacc
    16021 ggggcaccct cgcgctggtg atcgggctgc acgccctggc ggcgctcgcg tcgttggcgc
    16081 tgccgctgct cgtcgggcgg ctcatcgacg acctgcgcgc gggcacgacg cgcggccagg
    16141 tggaccggtt cgtcctgctc ctgctcgcca cggtgatcgt ccagacggcc ttgatgtggt
    16201 tcgctcggcg ggcagccttc gtcttcggcg aggggctctt cgccgacctc cgggagcggt
    16261 tcatcgaccg agcctcacgg ctcccgctgc ccgtgctcga gcgggccggc accggcgacc
    16321 tcgtggcgcg gacgacaaat gatgtcgaat ccctttcgtg ggcagcccga ttcgcccttc
    16381 cgtccatatt cgtcgcgctc gtgacggtcg tcatcacggt tgccgcgacg gttgtcgtct
    16441 cgccgctcgg cagtctcgcc gtgctcgccg tgctgccgct cgcgctgccc gccacgtggt
    16501 ggtacttgcg ccgtgcccgg accggctacc tgtgggagcg agcagcgtgg gcgcagctca
    16561 acggagtagc cgccgagtcg gccgactccg cccgcaccat cgacgccctc gacctcggcg
    16621 acgtccggca cgggcggctg ctcgagtcgg tgcgcagggc ctatcgtgcg gagtactaca
    16681 cgcgctggct gcgcatgtgg tggttctccc ccaccgagtt ctcgtatgtt gtgccggttg
    16741 ccgtcgcgct cgtgtggtgc ggctggctcg tcgcggacgg cgcgatgacc ctcggtgcgg
    16801 cgacgacggt catcctctat ctccgccagc tcatcgaccc gctcgacgag ctgatcagct
    16861 ggctcgacga gatccaggtc gcggcgacct cgctggcccg gatcatcggc atcgagaatg
    16921 tgcccgacga tcgtcggccc tccggcgcga ccccggcgga cgacgcgatc tcggctcgcg
    16981 acgtgcactt cacctatcgc gcggacaacc ctgtcctgca cggcgtttcg cttgatctca
    17041 tcccgggtga gcggctcgcc atcgtcggcc cgtcgggtgc gggcaagtcg accctcggcc
    17101 ggctcatcgc cggcatcgac gcgccgggat cgggcacggt gaccgtcggg gcggtgccgc
    17161 tgatggagtt gtcgatggag gacctgcggg gcgaggtggc cctggtgacg caggagcatc
    17221 acgtcttcgt cggcaccctg cgcgacaacc ttctgctggc gaagccgcgc gcgagcgacg
    17281 ccgacctgcg caccgcactg gcctccgtcg acgcgctgga gtgggcgacc ggcctgccca
    17341 cgggcctcga caccgaggtc ggctccggcg gtcacgagtt gacgcacccg cagagccagc
    17401 aactcgcgct ggcgcggctc gtcctggccg atccgcatac ccttgtcctg gacgaggcga
    17461 cctcgctgct cgacccccgc gccgcacggc acctggagcg ttcgctcgcc aaggtcgtcg
    17521 acggccggac ggttgtcgcg atcgcccacc ggctgcatac ggcccacgac gccgaccgcg
    17581 tcgccgtggt cgaggacggc cggatcagcg aactcggctc ccacgacgaa ctgctcgccg
    17641 ccgacggccc ctacgccgcc ctctggcgct cctggcgcgc cgagtaacct cctgcactct
    17701 cgacccgagt tacgtgttca ctcgacgggg agggccttcg tcgagtgaac acgtaacgct
    17761 cgtcgagtga acacgtaacg ctcgtcgaga ggacagtcct aggcgacgac ggcagggtgg
    17821 gcggcggggt cgccgagggc cggggcgatg ggagccaagc ggacgtaagc ggacccttgc
    17881 ggcgggcgct gatcggcttc tccggccttg ggccacaacg acattgcgcg ttcggcctgg
    17941 gcggtgatgg tcagcgacgg gttcacgccg aggttggcgg agatggccga gccgtcggtg
    18001 atgtggaggg tcgggtagcc ccatacgcgg tggtaggcgt cgatcacgcc gtcctgcgcc
    18061 gtggcgccga tgacggcacc gccgaggaag tgcgcggtca tcgggaagcc gagcagttcc
    18121 gcccacgagc cgccgggtat ggcgcgcacc cccacccgct tgcccagcgc ctcggcgagg
    18181 gcgcgggtgc cggcgtggcc ggcggggatc caggacgggt tcgcctcgcc atggccctgc
    18241 cgggaggtca gcgtgcgccc cccgagccgg ccgcggcgtc cggagacggt gaccgagttg
    18301 tcgcgggtct gcatcacgag ggcgatgacg gcgcgctggg accacttgta gttgttgagc
    18361 gccagcagct gaccggggtc cttggccatg acgcggaaca tctccagcat ccgtggccgg
    18421 cccgtatctc ccggcacgag aaggcttttc agcaagccca tggagttgga cccggggccg
    18481 tagcggacgt tctcgatgtg cgtctcgtgg tcgggatgga aggaggaggt gatggcgatg
    18541 ccgtggctga ggtcggcgtc gggcgtgtac ttcgccagct gcgcgccgag cagcgcctcg
    18601 gagttggtgc gggtcagctc gcccaggcgc ggcgagagcc ggggcatgat gccgcggtcc
    18661 ttcatcgcgt ggagcagggt ctgggtgccc caggtgccgg cggccaacac gacctggtcg
    18721 gcggtccagg tatgccggtc gggccgcttg gccccggagc gctgggtcac aacgcgccac
    18781 cggccgccct ctcccgcagg cggattcagt tcggtcacct gacgcatggg ctcgatgacg
    18841 acaccgagcc gctcggccag cgcgaggtag ttcttcatca acgtgttctt ggcaccgacg
    18901 cggcagccga ccatgcagtt gccgcactgc gtgcacccgg tgcggtcggg tccggccccc
    18961 ccgaagtagg gatcggcgac ccgctcccct ggcgttccga agtagatccc gaccggggtc
    19021 ttgcggaagg tgtcgccgac acccatcgac tcagccgcgt cacgcatcgc cgcctcgacc
    19081 gggccctcgc acggattggt gacgaccgcg agcatacggg tggccgtctc gtaatgtggc
    19141 gtcagctcgg cctgccagtc ggtgatgccc gcccactgcc ggtcctggaa gaagggcgac
    19201 ggcggcacat agagcgtgca cgcgtagttg agcgacccgc cgccgacccc cgcgccggcc
    19261 aggaccagga cgtcgggcag cttgtggatg cgctggatcc cgaagcaccc cagctgcggc
    19321 gcccacaagt agttgcgcag atcccagttc gtcttggcga agtcctcgtc ggagaaccgg
    19381 cgtccggcct cgagcacgag cacccgatag cccttctcgg ccgccctcag ggcggccacc
    19441 gacccgccga agccggatcc gatgacgacg acgtcatagt gatcgccgct cgtcgccgga
    19501 gccacgccgc gacgaactgc cttggcacgc aacatcgtca gcttgctcat gctcggcgcg
    19561 ccgccttcag tgcccgcagc gccgaggtca gcgtcttggc gaacttctcg tcgtccatcc
    19621 cgaagaccgg cgcgatcggc agcagtcgct gagcggtgac attctgcgat tcggtgaacc
    19681 gcaggatgcc ctcatcgccg tggcggcgcc cgatcccgct cgccttcatc ccacccatcg
    19741 gggcggccac actgccccag gcggcggcat acccctcgtt gatattgact gtgcccgtgc
    19801 ggatccgggc ggccagcgcc ctccctcgcc gcacgtcgcg ggtccagacc gaggcgttga
    19861 gaccgtattc ggtgtcattc gccagcgcga ccgcctcgtc gtcgctcgcc acgcgataga
    19921 ccgacaccac ggggccgaag gtctcctcgt tgcggcacgc catctcggcc gtgacaccgg
    19981 agaggacggt cgcctcatac gcaaatggcc cgaggtcggg gcgaggccgc ccgccggaga
    20041 gcaccgtggc gcccttggcg acggcgtcgt cgacgtgccg ggtcacggcc tcgagttgct
    20101 cggccgaggc gagggaaccc aggtcatagc cgtatgtcag cgcggacccc atccgcagcg
    20161 cagcggcggc cgcggcgaag cggggcacga aggcgtcgta caccttctcg tgcacgatga
    20221 cccgctcggt ggagatgcac aactgtccgg ccgaggcgaa gcaggcgcgc acggccccgg
    20281 gcacggcccg gtcgaggtcg gcgtccgccg cgacatacag agagttcttg ccgcccagct
    20341 ccatcgagca gccgacgagc cgctcccccg ccgcctgcgc gatgcgccgg ccggtggccg
    20401 tggacccggt gaagcagacg tagtcggcgt gctcgaggac cgcgttgccg acagtcgagc
    20461 cgcgtccgag cacgacctcg agggcgccgt cgggcaggcc ggcctcgtga agcaggtgca
    20521 cgaccgcgag cgcggtgagc gagccctgca tgtcggggcg caggacgacg gcattgccgg
    20581 ccatcagcgc cgggatggcg tcggtgatcg cgaggctgag cggatagttc cagggcgaga
    20641 cgatgccgac gacgcccttg ggcagatagg actcgatgct ctgggtgagc accggatagg
    20701 cgccggcgcg gcggcgcggc cgcagcgtct tctcggcggt gagagcgtag tggcgggcga
    20761 cgatggcggt gtcggcgacc tcctcgtgcg cctggctgcg cgccttgccc gactcgagct
    20821 ggacgaggtc gagcagctcg gactggccgt ccaggagcag gtcgtggaaa cgcagcagta
    20881 tgtcgcggcg tgcctcgaag gtcgttgccg cccagcgctt ctgggagctg cgggccgcgg
    20941 tcaccgcacg ctcggcgtcc tccggcgtcg acagcggcaa ggtggcgatc acctcgccgg
    21001 acatcggggt gcgcacctcg aagtgctccg gctcccggct gcatacgacc aattcggccc
    21061 aggtccggac ccgctccgga tcgagggcga agccgggggt cgcctgagca ggggtcatcg
    21121 tcgccgtcat acctcgcagg ttactgccag caggtggccc cgccgagcgc cgagtagccc
    21181 ctgtcacacc ccgggatggc cggggcggtg cggccgagcg acccgagccg cccgagaagc
    21241 ggggcctggt ttccgtacac gggcctggtt atagcccggc ccgcgaacga gaacccggcc
    21301 ccgcttcatc cgaacccggc ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21421 nnnttatagc ccggcccgcg aacgagaacc cggccccgct tcatccgaac ccggcccggc
    21481 gcggtgatga cgcaggtgga cccgtccggg tgcggcgcca ggctcagctg cgcacgtgcg
    21541 gggagacgac gacgtcgatg cgctggaact ccttcagttc cagatagccg gtcgtcgcca
    21601 tcgcgcggcg cagagcaccc atcaggttgg tcgtgccgtc ggccgaacgg ctcgggccga
    21661 agaggatctc ctgcaggctc ccggccgtgc ccacctcgac cagctctccg cgcggcaaat
    21721 cccggtggtg cgcctccggg ccccagtgga aaccgcggcc gggcgcctcg gtggcacgag
    21781 ccagggcagc gccgagcatg actgcgtcgg cgccgcaggc catcgccttg acgatatcgc
    21841 cgctgacgcc catgcccccg tcggcgatga catggacgta gcggccgccg gactcgtcga
    21901 ggtagtcgcg tcgggcgccg gcgacgtcgg cgacggccga cgccatgggc gcgtggatgc
    21961 cgagggcggt gcgagtcgta tgggcagcgc cgccgccgaa accgacgagc acgccggccg
    22021 cgccggtgcg catcaggtgg agggcggccg tgtagctcgc cacaccgccg acgatgacgg
    22081 gcacctcgag ttcgtagatg aaccgtttga gattcagcgg ttcggcgcgc gaggaaacat
    22141 gctccgcgga gacggtggtg ccccggatga cgaagatgtc gacgtggctc tcgacgacgg
    22201 agttccagaa ctgctgggtg cgctgcggcg acaaggcccc ggcgacagtc acccccgcgt
    22261 cgcggatctc cttcagccgc gcgccgatca ggtcgggctt gatgtcctcg gcgtagatct
    22321 cctgcatgcg cgccgtggcc agcgccggct cgagcgtggc gatctccgcg aggaggggct
    22381 cgggatcctc ataccgggtc cacagtccct cgaggtcgag caccggcagg ccgccggcct
    22441 ggccgaaggc gatggccgag gcgggcgaca tgaccgagtc catcggtgcg gcgagcaccg
    22501 ggatctcgaa ccggtaggcg tcgatctgcc agtcggtgga gacgtcctcg gggtcgcggg
    22561 tgcgccgcga gggcaccacg gcgatctcgt cgaaggagta tgcccgccgc ccgcgcttgc
    22621 cgcgcccgat ctcgatctca gccacccgcc aaggctatcc gccgccgaca ccgcataccg
    22681 tcaccgtcca ctcccctgcc gtgacatctc gacggggccg aatcgacatc tcgacggggg
    22741 gcgtcgagat gtcgccaggt cagcggccgc ggtagttggg ggcctcggcg accatctgca
    22801 cgtcgtgcgg gtgggattcc ttcaggccgg cggcggtgat gcgcacgaaa cggcccctgg
    22861 cctgcagctc cgggaccgtg cgggcgccga catagaacat cgactggtgc agccccccga
    22921 tgagctggtg ggtgacctgg gcgaccgggc cgcggtaggc gacctggccc tcgatgccct
    22981 cgggcacgat cttgtcgtcg gattcgacgt cggcctggaa gtagcggtcc ttggagtagg
    23041 acttcttgcc gcgcgagctc atggcgccga tcgagcccat gccgcgatag gccttgaact
    23101 gcttgccgtt gatatagacg gtctcgccgg gggtctcctc gcagccggcg aacaggccgc
    23161 cgatcatgac ggtgtccgcg ccggcgacca gcgccttggc gatatcgccg gagaactgca
    23221 ggccgccgtc gccgatgacc ggcaccccgg ccggcttgca cgcctgggcg gcctgatgga
    23281 tagcggtgat ctgcggcacc cccacgccgg ccacgacccg ggtggtgcag atcgatcccg
    23341 gtcccacgcc gaccttgacg gcatccacgc ccgcgtccac gagcgcctgg gcgccctcgc
    23401 gggtggcgac attgcccccg atgacctgca catgccgcat acccgggtcg gacttgatcc
    23461 gccggaccat ctccagcagc gcccgggcgt ggccgttggc ggtgtcggcg accaggacat
    23521 cgacgccggc gtcgacgagc gtcgtcgtgc gctgccaggc gtcgccgaag aagccgacgg
    23581 ccgcggcgac gagcagccgc ccgtcggcgt ccttgctggc gttggggaac tgctcggact
    23641 tcacgaagtc cttgaccgtg atcagcccgg ccaggcgacc ctcgtcgtcg acgatcggca
    23701 ggcgctcacg cttgttctgc cgcagcaacg cgatggcggc gtcgcgctcg atgccgaccg
    23761 gggcggtgaa cagcggcatc ggcgtcatca cgtcggcgac cttggtgctc gcccactcgg
    23821 cgaccggggt gaagcgcagg tcgcggttgg tgatgatgcc gaggagctgg ttgtcgacgc
    23881 cgacgaccgg caaacccgag acgcggtact ccccgcacac ggtgtcgagg tcctcgaggg
    23941 tggcgtccgg gccgatggtg atcgggttgg agatgatccc ggtctgggtg cgcttgacca
    24001 ggtcgacctg gtaggcctgg tcctcgatcg agaggttgcg gtgcagcacg ccgatgccgc
    24061 cctggcgggc catggcgatg gccatccgcg cttcggtgac cgtgtccatg gcggccgaga
    24121 ccagcgggat ccggagcgag agctcgcggg tcagccggct ggtggtgtcg acctcgctgg
    24181 ggatgacgtc cgtctcacca ggcagcagca ggacatcgtc atatgtgagt ccgaggagcc
    24241 cgaacggccc cccgtggtcg atcgcatcga tgggagcctc gctcgtcatt ccccaatggt
    24301 acggggtgct ctcggccctg caccggcggc gaaagctgcc acggcgccgg acatataacc
    24361 aatcctttgc cattttctaa cttgcggctt gcacaacgga ctggacatcc gtcatacggt
    24421 ccggttccag acgttgccga ggcagcagtg ggaccccggc gacccgagcc gaaaggggtg
    24481 tccgcgcaca tgcccgacct gatgaccgtc ccctcgcccc ttgccaccga atgggattgg
    24541 caacaccgcg ggtcctgccg gagcctgagc ccggagatct tcttccaccc cgagggtgag
    24601 cgcggctcgg cccgccgggt ccgggaccgg gcggccaagg aggtgtgcgc cgagtgcccc
    24661 gtgctggctc gatgccgcga gcacgccctg cgggcccgcg agccgtatgg agtgtggggc
    24721 ggcatgagcg aagacgagcg tctcgccttc tacgaggccc acgggcttcc ggtccgccgg
    24781 gccagctgag caccggaaag ccttacaccc gtgggcggtt cagcccgcgg gtgcgggcgc
    24841 gccattcgcg gcgcagcgcc tcccgccacg gcgggaccac ctgcccctgg gccgccagga
    24901 cccggcgggc gcggccgcgg gtgaggtaca tgccgacgat gcccacggca tacagcggaa
    24961 gttgcacggc catcgcgagg cggaactggc cgagcgtgta gtccggcccg tcgccggcgg
    25021 cgtccaggac caggccgatc agctgcgtcg cgatgacgca cgcggtgaac ccgccgaggt
    25081 tgaccaccgc gttggcggcg ccgaggcgat gaccggggtt gaaggtgcgg gcgaagtcga
    25141 acccgatcgc cgaacccggt cccccgacgg caagcccggc gcacaacgcg acgagcagcc
    25201 acagcggggc cgggcccggc cagagcagca ccagcagcca cggcaccagc ccggcggcca
    25261 ccatcgcgag cacgagattg ctgcgccgca gcggatggcg ctgcaccagg acccccagca
    25321 tcgggccgac gatcgcaccc acgacggcca ggaggccgat gagcaggctg gcggtcggtt
    25381 tggacacgcc ttccccttgg gtcagatagg ggaagcccca catcatgccg aagaccatgg
    25441 gggcgaagca ggtggtccag tgtgtccaca tccccagcag agtgcccgga tgccgcgctg
    25501 ccgcacgcag attcgcgcgc atgccgacgg caggccggac gggggcgggt atgccgggtg
    25561 gccggtcgcg caggaaggtc caggagagcg ccgccgcggc gagcgccgag agcgtgacgg
    25621 cgaggaaggc ggtgcgccag ccgcgggcgt cgagcagcca cgcgaacggc agggcgctga
    25681 cgatctgccc gagctgcccg gacagcccga cgaactgggt gacgatcggc acccgccgcg
    25741 gcgggaacca ggacggcagc agccggatga cggaggagaa gacgaaggcg tcgccggcgc
    25801 cgaggatcat ccggccgggg atggcgatgg tcaccgagtc ggcgaacgcc atgacgatct
    25861 gcccgatgcc catcgtggcc aacccgagcg tgagcatccg gcgcgagccg tagcgatcga
    25921 gcagcacacc cgccgggatc tgcatcgcgg catacaccag caactgcagc acgacgaagg
    25981 tggcgatgat gctcgcccgg gcgtggaagt gctcaaccgc cgacagcccg gcgacgccga
    26041 gcgtcgtgcg gccgagtacc gccgtgccgt agccgacgac gccgaggccg aaggtgaccc
    26101 acgcccgcac gccccccagg tgcgaaggtc ggtcggcgcg ggcggtcacg agggatcagt
    26161 ctccccgatc gtgcgcggcg ggcggaaatc cagccgggga cgcggcggct gcggcggcgc
    26221 gacggcgtga tctgatgtcg gagtgcgagt gcgggtggcg acggcgggtg atgtcgaggc
    26281 gctcgtggag atgaagcggc agctcatcga atccttcgcg gcacccgacg gagtcgtatg
    26341 ccggctggca cgagcgggcg gctcgcgcac tgcgtgagat gttggggcgc gacacgatcc
    26401 ggtatgccgt ggcggaggtc gacgggcttg tgtcatgagc gagcctccac tgtcgatcgg
    26461 ccggacgacc tcatcgtgcc caccccacgc ccgccgcgga tcatcgtggt caccggcgtg
    26521 atcatggcgg gcgtcgagga cggtcgggtg ctcgagaccg aacacggtgt cggttatgcc
    26581 ttggtcgacc ttccgccgca ggtgccgacc ggcagccggg tgcacgtgcg ggggtgggtg
    26641 gaccacgagc ggcgtacgac gctcaaccag tggccggtgc tcgtggtctc gcgggtagag
    26701 gccatgcccg aggacgtgtc cgcggacatg acggggcccc cacctgaagg tgggggcccc
    26761 gcctaaccga tcgtgtcagc cgatggcgcc cgtcaggggt tgacggccac cacggtgtcg
    26821 gcttcgatcg tgccgccgat gcggtgggtg acgctgccga ggtattccac gccggcggtc
    26881 aggccactcc aaccgacgac cacgtcgacc ttgtcaccga cggccacgat ctgggggctg
    26941 cccgagacca ccgagaggct gccaccggtg gtggcgggga cccgccagct gcgcaggtcg
    27001 taggcgaccg gcgtggcgcc gacggcccag ccgtgcacga tcatcgtgta ggtgccatcg
    27061 gccggacgga ccaggtcgat ctgctcatcc gtgccgccat tggtcgactg cgccacgacc
    27121 ttgccgctcg agtcgagcag gtagatgtcg aggtcgacgt cgccgccgac gtcgagcttc
    27181 caccgggcgt ggtcgacgcc gttcagcgtg taggggatcc ggtcgactcc cgcgccgtcg
    27241 tcagggctcg ggtaggtctg gtccgggtcc tgcgccacgg tgtcgttgtg gtgttcggcc
    27301 ggcaccaggc cgtgtgcggt ggcggtgtag gtgcccgcca cgccgacggt cgaggtgatg
    27361 gtcgcctgac cggaggtgcc ggtgccggtg atctcggccg gtgacgcgat gcgggtggcg
    27421 ttcaccgcga tcggggatcg cacggcatac ccgctgccct gccacgtcag cgagccgaaa
    27481 cgccacgcgc cgatcggggc accggtgttg gtcaccgaca cctcgaagga cttgctggca
    27541 cccggagcga cggtgatcgt ggccgggctg acggtgacgc ggtagccctg cggagcccgc
    27601 acggacgcgg tcgcggtgat cgcctgcgag gagacgttcg tgacggtccg cttgacggtc
    27661 atcgtgcccg ggaccgcgga cataccgatg gacgggtagt tgaggttctc ggccgttgtc
    27721 gcatagccgg cgttggccag cccgccacac gtggcgtcgg ggttggcgaa ggcttcccgg
    27781 ccctcgtcgc acaggaaacc gaggtagtcg tagaagccgg cgtcatagac cagtccaggc
    27841 tggaaggagg tgctcttcgc gcccggggaa cccagcttcg ccatcccgga accggtgtcg
    27901 aacgggtcgg ccggcgtgac gcggtcgttc ttgcggaccg cggcgtagga ctgggtcatc
    27961 agggcggact tcgccgccgc ggcagaccac tcggggtggg cctgcttcag cagcgcgtag
    28021 gagcccgcga cgaccggcga cgacatcgac gtgccggcga tggcctgcca cagctgcccg
    28081 gcaggctggc tgccgccgct cggcatgggg gtgttgcccg ccatgatctg cgcaccgggg
    28141 gccgagatgt cgggcttgat ggtgtcgctg ttgggaactg tcgggccgcg gctggagaag
    28201 taggtcacgg tcgggctgta gtgccagagg gtgtccatgc ccaggttcgt aatccgcgcc
    28261 gtggcaccgg gggtgttgtt ggcgtaccgg cggatccgtg cgccgttggt gtagtccatc
    28321 atcaccgtcg ggacgaagaa gttgtccgag aacatcgtgt cgccatcggt ggagttgaac
    28381 agcaccatgc cgacgccacc ggcccgcttg acctcggcgc tcttggcgac gcgaccgttg
    28441 acaccgcgct cacacagcac gatcttgccg cgcaccttcg ccgcggacag gctgccctcg
    28501 acgcagtagc gcgagtccgt cccgcccgcg tgcgtgccgt cgatgatcgg cttgacgccg
    28561 gtgcccttgg tgacggatgt gcccttgaag acccggccgt cgccgagctt gacgcggccg
    28621 gcgaagttgc gggggaaggt ggtggcgccc acgccggtca cccaggggac attggcgggg
    28681 ccgccgaccg tctccgggcc ggggccgtcg ttgccggcgg acacggcggc gaagacgccg
    28741 gcgtccgcgg cgaacaggaa ggcgagggtg tcggcgctga cgacttcggc accgccgccg
    28801 atggagtagt tgatgacatc cacgccgtcg gcgaccgctt ggtcgatggc ggctgtcagg
    28861 tcggacccgt agccaccggc atcgccgagg gccttgtagg cgatgatctg ggcgcgcggc
    28921 gcgatgcccg agacgatgtc cttgttgacg ccgaagatcc gggactggac gttggcgttg
    28981 ccggccgcgg tcgtcgcggt gtgggtgccg tgcccctcgt tgtcccgggc ggagtcgaac
    29041 tcatcgtgct cgaggccggt ctgggacttg tagacgtcga ggaattcccg ggcgccgatg
    29101 agcttgttgt tgcaggtgaa cggcttgtcg ttggggttcc aggcctggtt gccgaaggca
    29161 cacgcggagc gctcggactc atcgagcgtc ggtgcggcgg ggaaggtgcc gttgtccctg
    29221 agcatggggt gctcgggcca gatcccggtg tcgatcacac cgacgatcac gccgttgccc
    29281 ttgatcccct tggcgtaggc accggtgtcg agcccgagga acttctgcag gtcgttgacc
    29341 ccggacggcg cggccttggc ctcgttgact gccgcggcga tcgccgtggg ctggcgcatc
    29401 tcgtccttga ggaccgcagc gacgcccggt tgcttggcga gcgcggcggc acccttggcg
    29461 tcggtcagca ccgagaagcc gttgagggcg ttggtatagg tgttgacctt ccgggagacc
    29521 gcaattcctg cgcccttgag gaccgtgtca tggctcgcct tcaacgcggc ccgcttgctt
    29581 tgggccccgc tggtgcgcag gtcgctctgc ttggtcgacc gcagcaatgg gtcggccttc
    29641 atgacgacga tatagctgcc gccgacaccc ttgccggacg gcggggtcgt ggcattcgcc
    29701 ccgcctgcgc tcgcgccggc gaacgcgacg gcggtggccg cgactccggc gaacgcgatg
    29761 attcgcttca cagtgtggat ctcccttcgg gaacaacggc ttcgccggcc cacgagcgaa
    29821 gccccccgac cgcatcgacg cgaccggacg agcgaaggct aacgcccggt agcgcacgtc
    29881 ggctaggcct tcccccgcgt gtctcgccga acccggcgga gcccacggct gcacataacc
    29941 cgtcacgagt cggggaatgt ctccggccac atcccccaac tcgtgacaac acagcagatc
    30001 agcacggcgg atctgacgac cacggccccc ggatggaccg cgcgagcggg ccatccgggg
    30061 gccatgagga gtcagatcga gaagacgacg agcatcagtg cccgtggccg tgaccggccg
    30121 cgggagcggc gggctcctct tccttcttct ccaccacgag ggcgtcggtg gtcagcacca
    30181 tcgaggcgat cgacgcggcg ttgcgcagcg ccgaccgggt gaccttgacc gggtcgacga
    30241 caccgacgcc ggcgaggtcg acatactcgc ccgtggcggc gttcaggccc tggccgatcg
    30301 ggagctcgcc gaccttggag acggcgacat agccctccat accggcgttc tcggcgatcc
    30361 agcgcagcgg ctcggcggcg gccttgcgga cgatattggc gccggtgagc tcgtcaccgg
    30421 tcaggcccag gtcgtccagg gcgggcagcg cgtggacgag ggcggtgccg ccaccggcga
    30481 cgatgccttc ctcgatcgcc gcgcgggtgg cggagatcgc gtcctcgatg cggtgcttct
    30541 tctccttcag ctccacctcg gtgtgcgcgc cgaccttgat gacgcagacg ccgccggcga
    30601 gcttggcgag gcgctcctgg agcttctcgc ggtcccagtc ggagtcggtc ttgtcgatct
    30661 cggccttgag ggtgttgacc cggccggcga cctcgtcggc ggacccggca ccgtcgacga
    30721 tggtcgtggt gtccttggtg acgacgacgc ggcgggcctg gccgagcagc tggaggccgg
    30781 cctggtcgag cttgaggccg acttcctcgg agatgacctg gccaccggtg aggatggcca
    30841 tgtcctgcag catcgccttg cggcggtcac cgaagccggg cgccttgacg gcgacggcgt
    30901 tgaaggtgcc gcgcagcttg ttgaccacga gcgtggacag cgcctcgccg tcgacgtcct
    30961 cggcgatgat ggccagcggc ttgccgctct tcatgacctt ctcgagcacc ggcagcaggt
    31021 cggcgatcgt ggagatcttg ccctggttga tcagcaggta gcagtcctcg aggacggctt
    31081 ccatgcgctc ggggtcggag accatgtagg ggctgatgta gcccttgtcg aactgcatac
    31141 cctcggtgaa ctccagcacc gtctcggcgg tcgaggactc ctcgacggtg atcacgccgt
    31201 ccttgccgac cttgtcgaag gcgtcggcga tgatcgagcc gatgccgtcg tcctgcgccg
    31261 aaagcgctgc gacagaagcg atatcgtcct tgccgtcgac ctcgcgggcg ttctcgagga
    31321 ggcgggcgtt cagtgcctcg acggcagcgt ccataccgcg cttgaggctc gcgggcgagg
    31381 caccggccgc gacattgcgc aggccctccc tgaccatcgc ctgggcgagg acggtcgccg
    31441 tggtggtgcc gtcaccggcg atgtcgttgg tcttggtggc gacctccttg gcgagctggg
    31501 cgc
//
LOCUS       DAOPJG010000015        31042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1552_length_31042_cov_8.012425, whole genome shotgun sequence.
ACCESSION   DAOPJG010000015 DAOPJG010000000
VERSION     DAOPJG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 31042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31042
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1552_length_31042_cov_8.012425"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKU75_02905"
     CDS             <1..777
                     /locus_tag="PKU75_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HPF79853.1"
                     /translation="NAFMVLACLMDLRRPRPRLLRALPPGALVGFAMAIKLTPGVFVI
                     HYVLSRRWREAFTAVGAAALAWLTAFLILPEASWAFWGGALQDPARLGPNMGTSNQSL
                     RGVLMRIGPDGALGTGIWLVCVLAVGAYGFGIAQRAHRAGDSITEVAAVGLMACLLSP
                     VAWIHHFHWMVVVILAVLGAAPWRDRRRLVAAGVLLVWFTCRLPWWGISWLGNGWSPD
                     FVGRLLQNADLIGGLLALWLLHWSLARDLDEAATRERGQV"
     gene            complement(859..1470)
                     /locus_tag="PKU75_02910"
     CDS             complement(859..1470)
                     /locus_tag="PKU75_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HPF79854.1"
                     /translation="MTFSIATAAIVFALIFPVELPDKTFIATLVLATRYRPLLVWIGV
                     ASAFAVQTVVAVTLGGLLAKLPHQPVQILAACMFALGAVILFRGASAADEEEHEVEEE
                     FAAKVAVPETGFRAVVASFLILFLAEWGDLSQLLTASLAAKYQDPVSVGVGAFLALAT
                     ISALGAALGRQLLKRMKLATIRRVGASVCLLLSALTVVELVRG"
     gene            1506..2435
                     /gene="pheA"
                     /locus_tag="PKU75_02915"
     CDS             1506..2435
                     /gene="pheA"
                     /locus_tag="PKU75_02915"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPF79855.1"
                     /translation="MTRYGYFGPSGTFTEMALLDWPPARDAERVPFASVDATLAAVRG
                     GDVDAAMVPIENSVEGGVSATLDALATGSPLIVIAERLVKIRFVLAAREGMTLERVAT
                     IGTHSHAWAQVRGWVGANLPEVTYVPTLSTASAATDLAPGGEGRHDAAICAPVAAAQA
                     GLQVLADDIGDIKRAVTRFVLVARPNRIPAPTGADKTTVVLYQRKDMAGGLLRLLDQF
                     ATRGINMTRLESRPTKHSMGDYCFSVDIEGHVLDERVGEALTGLKRVCAEVVFLGSYA
                     RADNGRAQVAPFNGDDDFRAAARWLDAIRDLVQ"
     gene            2586..3422
                     /locus_tag="PKU75_02920"
     CDS             2586..3422
                     /locus_tag="PKU75_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HPF79856.1"
                     /translation="MLVSNALVGLREGLEASLVVVILIAFLVKTDRRWALKWVWAGVA
                     TAVVLSVALAGFLTYGTRQLSFKTQELIGGGASILAVAFVTAMIFWMRRAARTISGEL
                     KGSMDRALSAGGVWVALVGFLGVGREGLETAIFFYATAQAAGAGTSQPLIGWVLGILG
                     AVVIGLLMYRGAIRFDIGKFFRYTGIFLIVVAAGILAYGIHDLQEAAFLPGLNSHAFD
                     VSSIIEPGGWVATLLKGIFNFTPATTWLQAIAWTAYIAVVLPLFLRPVAAPAARPVPS
                     AA"
     gene            3456..4613
                     /locus_tag="PKU75_02925"
     CDS             3456..4613
                     /locus_tag="PKU75_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M75 family protein"
                     /protein_id="HPF79857.1"
                     /translation="MQSRSPLRSALLLAGAASLTLTACGGGASGDAGSGSAGAGGPIT
                     VTATDTECTVSANTAPAGKVTFTVTNKGTKINEFYVYAEGDRIVGEVENITPGLSRDF
                     IVEVSEPGSYETACKPGMVGNGIRSAFTVTGDAPAAKTDDQDLQAAVAGYSRWVNSQG
                     DAFLTRTTEFVELVKAGKVEEAKALYPVARSYWERIEPVAESFGDLDPKIDGREDVVE
                     EGMAFTGYHRLEKDLWVDGLQKDTSAIADQLLADVTELVTKAKSVELNPLQLANGSKA
                     LLDEIATGKITGEEERYSHTDLWDFEANFEGSKQAIAELRPYLTKKDAALVGKIDATG
                     KALGDLLETYRQGDGFVLYTDLTEDDVKKLTQALDAFSENVAQVAGVVAKA"
     gene            4610..5929
                     /gene="efeB"
                     /locus_tag="PKU75_02930"
     CDS             4610..5929
                     /gene="efeB"
                     /locus_tag="PKU75_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HPF79858.1"
                     /translation="MTDTPAPAPGADGAGASRRAAVGSALGAAAGVAAAGALYAANRA
                     DAATSPGQASTPHTAPPRGGDPIAFRGGHQAGITTPAQDRMHFVALDVTTESADELRE
                     MLTAWTRAAERMTYGAEAAPGGAVGLGPYGVPTDTGEALDLPPSNLTITIGYGPTLFE
                     RFGLEARRPDALEELPGFMGDDLDPATSGGDICIQACADDPQVAVHAVRNLIRLGFGV
                     VSVRWSQLGFGRTSSTSSSQATPRNLFGFKDGTANIMAEDTAALDEHVWVAPSDVSGE
                     AAWMAGGSYLVARRIRMHIEVWDRTNLQEQQDIFGRGKLKGEPLGGTDEFETIDFAAK
                     RMDGEPAIPMVAHVRLAHSSNLGGVRILRRGYNFTDGSDGQGHLNAGLFFIAFMRDAH
                     KQFVPMQRALAAKDVLNEYIEHTGSALFACPPGLGPDEDWGTQLFGR"
     gene            complement(5985..6989)
                     /locus_tag="PKU75_02935"
     CDS             complement(5985..6989)
                     /locus_tag="PKU75_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPF79859.1"
                     /translation="MGMRSIQPPPVLVRRALRVVWPLLALTITILGLPVLVAGALASV
                     VDRRARLFRATGLLILVVWLDVQLLIGCWRLRLADPRGASPSWHADHEQLLSDVLDKA
                     MALGRKWVGFEVRLDDVMDLGDPGKPLIAFARHAGPADSLAVAWLLSRTGGRLPRIVL
                     ADALRWDPGIDLILTRLGSSFVPSASGAGDDRLAGVRRLADSLARKDALLLFPEGENW
                     TPSRRGRLITRLREAGQHARARQAERLRNVLPPRTKGAIATLSARPDADVMIVAHAGM
                     GRLTGAREIYAAIPFERPFLVHTWTYPAETVPREPAAIESWLSARWAEIDAWVERQGE
                     "
     gene            complement(7005..7838)
                     /locus_tag="PKU75_02940"
     CDS             complement(7005..7838)
                     /locus_tag="PKU75_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPF79860.1"
                     /translation="MTERTAFVLGGGGVLGASQVGMMRALAEAGITPDLVVGTSIGAL
                     NGAFIASDPTVWGAERLASVWEEVVREGVLWERPVRKAAKIARHVTHLLTHAPLATLI
                     DRYLPVHEFEDLAVPFQCVAARIEDSSAAWFDAGPIAPAVLASCSVPGLFPPVRIDGA
                     HYLDGGLVHSIPVGRALELGATRVYVLQVGRVEQPLAVPRAPWDVATVAFEISRRHRY
                     VEELTRIPESVAVHVLPSGTSSAPTVSLSQARGRRVAERIEQAYAASTAYLAGDPVEP
                     D"
     gene            complement(7880..8548)
                     /locus_tag="PKU75_02945"
     CDS             complement(7880..8548)
                     /locus_tag="PKU75_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF79861.1"
                     /translation="MNSHEDKNTHEDKNTHIVIVGAGYAGVLAANRAAAKGHQVTLVN
                     DRDSFVDRIRLHEVIPGIRTPEAAQIPLEKAVRPRVRLVIAPAARIRSRTVELATGET
                     VRGDHIVVATGSGAGPDGLEWAEKNHAAVAGLPPGASVAVAGAGLTGVEVASEIAYAR
                     PDLRLTLADPIPVGTSYSDSGRRHLLPALERLGVRIASQTPDDTDLVVDCTGFATPPL
                     AGAR"
     gene            8752..9780
                     /locus_tag="PKU75_02950"
     CDS             8752..9780
                     /locus_tag="PKU75_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79862.1"
                     /translation="MSEPAFPMRTDRLVLRFVADEDIAPLTAYRNDPQVAALQDWDLP
                     YSEEKARALVAKHEGLTDLVAGRGHQIAIVRDGQLVGDVYVGIDEHGGVADIGFSLIP
                     AAQGQGIAHEATTALIDHVIERHGVHRITAELSTQNLASARLLERLGLTFESLTRQSF
                     WWRGVWDDNLYYSMTADERRAWRDRPTGPPADVRLVPITDDNRWAYSRLRVHRSQERF
                     VASVQDSYADAFFHGDRHGLPLIPQLRGIEADGEPVGFVMYSEATPYLWRFLIDRRHQ
                     GRGIGRRALSQWIDGMRTNGHTEIETSWVEGKGSPAPFYRALGFEPTGEFDEGETLAL
                     LRLGPTPR"
     gene            complement(9903..10364)
                     /locus_tag="PKU75_02955"
     CDS             complement(9903..10364)
                     /locus_tag="PKU75_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPF79863.1"
                     /translation="MPRPRRNRDDVRPRGHVALAAGIVAGSPDAAVGRESQDRDRGCA
                     YPGCTATRFVEIHHLQRGEDGGGTDYDNLICLCPHHHDAHHAGVFTIAASHNAVIPFT
                     FAARGGWAIHATPPEADDPGRGRPTDAGSYTPPLGERADYGWITYRRNDTA"
     gene            10546..11973
                     /gene="zwf"
                     /locus_tag="PKU75_02960"
     CDS             10546..11973
                     /gene="zwf"
                     /locus_tag="PKU75_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPF79864.1"
                     /translation="MAAPLTVILFGATGDLSKRKLIPGMLHLFQSRLLDDVRVVGTSL
                     DDISLEEFRDLALTAIKEHSTRSLNDEDWNAFAQRLDYVPLSAGPEALRAAVLRSEEL
                     LPGDHELRVHYLSVPPKAALAAVSTIAAAGLVERSRIIMEKPFGIDHASSVKLNAQLH
                     EVFDESQIFRIDHFLGKEAAQNILAFRFANGLFEPIWHRNHIDHIQIDVPETLGLTQR
                     GDFYESTGAYRDMVVTHLFQVLAFTAMEPPTALEPAAINREKTKVFRSMLPIEPSNVV
                     RGQYVGYKDEPGVKPESETETFVALKCYVDNWRWAGVPFYLRTGKRMAQGARIISIAF
                     KEPPRSMFPAGSGVGDHGPDHLTFDLSDQPKMSLSFYGKHPGPGYRLDKLSMQFSMEE
                     TSWAGAVLEAYERLIYDAARGDSTLFTSATGVERLWEVSTPLIENPPIVRPYDQGSWG
                     PNQIHQLIAPFSWRLPFERKWRDGT"
     gene            complement(12023..13189)
                     /locus_tag="PKU75_02965"
     CDS             complement(12023..13189)
                     /locus_tag="PKU75_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPF79865.1"
                     /translation="MVPNALMLAAAVALNVTLLAVAIVLALNGRPGRHAKPRPHRDAF
                     RGAATTPPRRKRAAVIVNPTKFTTLTPVRSALGSVAAELDWDQPLILETTREDPGYGQ
                     ARSALDAGVDLICSLGGDGTVRVVASVLAGTSTPMGLLPGGTGNLLARNLELPLTDIG
                     EAFRVACAGRNRAVDVAYAALAPPPSPAPHPGEQEAGGQGAGDSTEAADAAAEHAFLV
                     MAGVGLDATIMSETSEESKAKLGWTAYAATGLKNFLGTRIRADVSVDGGPARSVTAST
                     VLIGNCGRITGGINLMPDARIDDGVLDVVVLSPKGFPGWVDVTARVLTQHRSNRTERL
                     QRHTATEVVIATDKAQAVQLDGDVIGETTRLSARIVPKALLVRVAALGEPGPAG"
     gene            13264..13830
                     /locus_tag="PKU75_02970"
     CDS             13264..13830
                     /locus_tag="PKU75_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPF79866.1"
                     /translation="MPDPTSQTPPTPAPDAKDWTWVIEQPCPDCGFAGALIAPGEFAI
                     RIAAATAPWQEVLRRSDVRDRPAPTTWSPLEYSCHVRDVCRIFDERTRLMLTEIDPGF
                     ANWDQDATALEERYWEQDPAQVAADLATAATQLSRTYAAVPADAWGRTGRRSNGSVFT
                     VTTLGRYLLHDLVHHLWDVGAAPLAREK"
     gene            13836..14492
                     /locus_tag="PKU75_02975"
     CDS             13836..14492
                     /locus_tag="PKU75_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HPF79867.1"
                     /translation="MPPAHLWFSFLIAGLVLCLIPGPSLLFTIGRALAEGRRTALLSV
                     LGNGLGILTQILAIAVGLGPLVAKSGALYTTLKLAGAAYLVSLGVQTIRHRRDAESAL
                     AILDAPRRPAHHAVRDGFVVGVTNPKTIVFFIALLPSFVDPAGAPVPLQMAVLGVTFF
                     LIGITCDGLVATAAGTARQWFARQPNRMRQLSAAGGVMMIGLALALALTGRPGEAHAR
                     "
     gene            14514..15761
                     /locus_tag="PKU75_02980"
     CDS             14514..15761
                     /locus_tag="PKU75_02980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF79868.1"
                     /translation="MEPPGALRVVFVSMHTSPVADPGSGDVGGMNVVESALADALADR
                     GHQVEMITRRADTESPDIRELRPGVTLRQVPAGPPTPLPKSVIDDHIDEFAAGLRAVA
                     RPDIVHSHHWMSGVAALAWAREMGVPHVQSFHSVAAPPNRPLSDGEPPESPRRVDGER
                     VVARLSDTIVAISVAEARTVVDRCGADPDRVVIVRPGVDLDLFHPADTRTPDANPTEG
                     NGAASAGATGDNGYVMFIARLQPLKGPDLALRALSHIEQWQRPHLLVTGDVSEDFAEY
                     AAELHELVRRLGLSDFVTFTGAVDRPRLAQLLRDAELLLVPSHSETFGLVALEAAASG
                     VPVLAAAAGGLREAVAHQETGQLIDSREPEDWGRAMRHVLHTPGLRASMGRAARIHAR
                     RFEWRWSAARLEDVYGDLLGRST"
     gene            15758..16528
                     /locus_tag="PKU75_02985"
     CDS             15758..16528
                     /locus_tag="PKU75_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPF79869.1"
                     /translation="MSLLLDLRGQNVVVAHAHPDDETLATGALLAEIASAPGEVHVVT
                     ATRGERGELMPGLPGRAADLVPGSWSYVAYREGELAAALSALGIRHHAFLGQPPARAE
                     GLPSRSYADSGMRWVSPGVAGPAADVGADAFTAAGVGEAAGDLAAYLRAADADLVISY
                     DAGGGYHHPDHVRMHEVAKTAARECGLTFLEVIPPGRDDIPGDLDTIEWTDLSRHLPT
                     VQAALRAHASQVQVEGGDVVHVGGQREPIVTRVGLRRA"
     gene            16572..17840
                     /gene="serS"
                     /locus_tag="PKU75_02990"
     CDS             16572..17840
                     /gene="serS"
                     /locus_tag="PKU75_02990"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPF79870.1"
                     /translation="MIDIKLLADDPDRVKASQAARGDDPALVDQVLDADAKRRAALTE
                     FEQLRARQKSMGKEIASAQGEEKQKLLHETKSIAAEVKRLQATADEMVATRDELLMSI
                     GNVIIDGVPAGGEDDYVVRETIGTPRDFAREGFEPKDHLDLAEGLGAIDMARGARVSG
                     SRFYFMTGPGARLELGLLNAGMAMALEHGFVPMITPTLVLPEVMKGAGFIDRHADEVY
                     RLEADDLYLTGTSEVALAGYHTGEVIDLSGGPKRYAGWSTCYRREAGSYGKDTRGIIR
                     VHQFNKIEMFVYCDPAEAEAEHDRLLEMERAMLAKIEVPYRIIDTAAGDLGQSAARKF
                     DSEAWIPTQGRYRELTSTSNCTTFQARRLGVKMKGEKGNEVVATLNGTLATTRFLVAI
                     LENHQQPDGSVIVPEALRPYVGLDVLTPVA"
     gene            17854..18681
                     /locus_tag="PKU75_02995"
     CDS             17854..18681
                     /locus_tag="PKU75_02995"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPF79871.1"
                     /translation="MTTSHLVGLDVDGTILTHEGELREAVREAVHATAAAGHHVVIST
                     GRSVLGTLPVLADLGIDSGYAVCSNGAVTIALDPAHPSGYEILEAVTFDPRPALELLR
                     GAWSDAVVAVEDLGVGFKVSAPFPDRLEGVVTVVPWEELMADPVTRVTFHSPTGTSED
                     FTKLAERIGLHGVNYAVGFSAWLDINPEGVSKGSALEQIRRRLDVQPMHTVTVGDQRN
                     DVEMLTWAARGVAMGNAPDEVKAIAKEVAADVYSDGLAQVLRTLPGVGPSHQELIAP"
     gene            18678..19556
                     /locus_tag="PKU75_03000"
     CDS             18678..19556
                     /locus_tag="PKU75_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPF79872.1"
                     /translation="MTGLVISSPANPRIKAVVALRNRRDRDRAGVTRVEGREELGLAL
                     AAGARPRTLFYCPELMNANYSTAAQGPGMDLVAQVRALGTETIECTRAVFEKASYREG
                     PDGFLAVIDAPGRSLTDLDVPDGSLMLIAQGIEKPGNLGAMLRTADAAGVAAVIAADP
                     GTDWGNPNVIRSSKGAVFAVPVASATTAETLAWLGERGYALVATTPDTDLRHTDSDLT
                     GAVAIAVGTEKYGLDEEMFDAATVKVRIPMAGRIDSLNAATAAAIVLFEAVRQRLSVT
                     RTTQDPESTWGESWGP"
     gene            complement(19614..20249)
                     /locus_tag="PKU75_03005"
     CDS             complement(19614..20249)
                     /locus_tag="PKU75_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF79873.1"
                     /translation="MRVDHVSYAAEPDGLHATAERLAAKLGVTPVEGGVHPRFGTRNI
                     ILPLAHERYVEVVEVLDHPSADKAPFGQAVRQRSEIGGGWLGWVVRVDALSDLEERLG
                     RSAVDGNRRMPDGREFKWRQLGVRGLQSDPQLPFFVEWDADSPHPSDEAHTQVTMKSM
                     QIAGDPDRVKDWLGLPAETTSTVIHFDFVAPHGTPGLMSVTFNTPDGDVTV"
     gene            complement(20396..22735)
                     /locus_tag="PKU75_03010"
     CDS             complement(20396..22735)
                     /locus_tag="PKU75_03010"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPF79874.1"
                     /translation="MTMTETHTAPAETEEVDLAITGMTCASCSARIERKLGKLEGVEA
                     SVNLATEKAHVRFAAPHTIDEILGTVRATGYGAEIIAPRQPSRGGSTRHTAGPAGATS
                     HDALDHNAMDHNTMDHDGVHDRAVDHLDHDVAPHAVLKRRALISAIFTLPVVLLAMVP
                     ALGDVPGNAWWQFILTTPVVLWAAWPFHRAAAINARHRASTMDTLVSIGVLAAYLWSA
                     VGVLTGQAMTDMGMADKGVHLYFETAAVVTTFLLLGRWLEARAKAQGKSALTTLLDLG
                     AKDVAVERRNAVTGVVEEVRIPIDQLGVGEQFVVRPGEKVATDGVVLNGRGTIDAALV
                     TGESMPVDVAAGSEVTGGTINGAGRLLVRATRVGADTTLAGIARLVETAQTGKAGVER
                     LADRISAIFVPVVLGLALLTFVGWWIGSGSATKALAAAIAVLIIACPCALGLATPTAI
                     LVGTGRGAQLGILIKGPQVLEDTRRVDTVVLDKTGTLTQGKPTLIDVTPHGNLTKEQA
                     LRAAAAVEAGSEHPIAHAITSAATARNLQVPRASDFHALAGDGAKAVIAGQEVIVGRS
                     RLFTKMPADLPAHPEGTTVWVGWNGVAHASLTVADALRPDAEEAVRELEDLGLTAYLL
                     TGDAEGTARAVAQQVGIPAERVIAEVRPEDKHAMIERLQQDGRTVAMVGDGVNDAAAL
                     AQADLGIAMGSGTDVAAASADLVLMRSEVGSVAEGIGLSRATLRIIKQNLAWAFGYNT
                     AAIPLAMAGLLNPMIAGAAMAMSSVLVVTNSLRLRRFGR"
     gene            complement(22769..23050)
                     /locus_tag="PKU75_03015"
     CDS             complement(22769..23050)
                     /locus_tag="PKU75_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPF79875.1"
                     /translation="MAGYSGDKDDYLRRLKRIEGQVRGIARMVEDDTYCIDILTQVSA
                     VTRALQAVSLGLLEDHIGHCVVNAARESDEAADAKVTEAAAAIARLVRS"
     gene            complement(23142..24545)
                     /gene="purB"
                     /locus_tag="PKU75_03020"
     CDS             complement(23142..24545)
                     /gene="purB"
                     /locus_tag="PKU75_03020"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPF79876.1"
                     /translation="MPSLADTDAALGALDGRYRSAVAPLIDHLSEPALNRERLRVEIA
                     WLIFLTERGVIPGSGTLNEDEKSRLRAIVDEFGAADIEALAEIERVTQHDVKAIEYFL
                     KERLTAIAPAQAETMSELIHFGCTSEDVNNLSYALMIRGATRAVWLPKAQALLDQLEA
                     MARELRETPMLAHTHGQPATPTTMGKELAVLAWRLRRQVRRIDAAEFLGKFNGATGTY
                     GAHLAAVPDADWETISREFVESLGLTWNPLTTQIESHDWQAELYADLARFNRILHNLC
                     TDVWTYISFGYFSQVRGQGTIGSSTMPHKVNPIRFENAEANLEVSNALLDVLASTLVT
                     SRQQRDLTDSSMQRNIGTAMGHSLLALDNVSRGLAGLDANPEAMAADLDANWEVLAEP
                     IQSAMRALAAQGVPGMSNPYERLKELTRGRRIGQADLREFIGGLGLPDDVAARLRDLT
                     PASYVGLAATLVDHLGE"
     gene            24606..25070
                     /locus_tag="PKU75_03025"
     CDS             24606..25070
                     /locus_tag="PKU75_03025"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPF79877.1"
                     /translation="MGHPVRLLSASVDIAAPPATVWRIVTNVRRTGEWSPECRKVLAP
                     KTIGAGSRFVGINQRRLMLWPTNAKVVRFTPERELAYRIAENGSTWSYSLEPIDHGTR
                     LTEAREAADGITGFAHVFTKLFLGGEAHTSELDDGIRASLDRIKVIAERERP"
     gene            complement(25085..25615)
                     /locus_tag="PKU75_03030"
     CDS             complement(25085..25615)
                     /locus_tag="PKU75_03030"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HPF79878.1"
                     /translation="MTDNGVVTYRVCVICTGNICRSPMLEFLLREAFDREGLSDAVTV
                     DSAGTSSEESGNAADYRTLDVLQRNGHDDWGRAAHRARRIEKDWLAERDLVLAADSGH
                     LRALQRLAGGSPEHIRLIRSFDPAAVEAGELDVDDPWYAWNDKAFDRTYEEMQAAVPG
                     IVEHVKAELAQRSRTV"
     gene            complement(25626..26399)
                     /locus_tag="PKU75_03035"
     CDS             complement(25626..26399)
                     /locus_tag="PKU75_03035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPF79879.1"
                     /translation="MGITRRHFLGGAGACLLTATGGGAWAAHRQSAGPHTDSLARRLR
                     VEPGAIGRVETDEREVFLTFDDGPDPAYTPAVLDRLDQAGVSATFFLIGRNALAHPHL
                     VHEIASRGHVIGNHTQDHLWLDGLPQQQVHSQITACERSFASVAVATTGLFRSPRGWT
                     SDAVRASVDDIGLRSTFWTDCVEAFVRDHGPRGAAERIAGRMRPGSVILTHDGGALDG
                     PNAQRIDRSGSVAALPYLIDAIRDRRFTIRPLAGRSPVR"
     gene            complement(26494..28188)
                     /gene="cydC"
                     /locus_tag="PKU75_03040"
     CDS             complement(26494..28188)
                     /gene="cydC"
                     /locus_tag="PKU75_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HPF79880.1"
                     /translation="MTPDLRTVRLTRQLVAAGVIGGLALASGVALTATSGWLIVKASE
                     RPVILTLMVAIVGVRAFGMARPAFRYWERLITHDAALRDLAQRRVDTYAALIPLTPAR
                     LGRRGRADLLTGVVDDLDDVVGAQIRVTVPGLSALIAWGITTAVTTAFDARVGLCIAV
                     LGLVVTALSLIAFRVESAGNAALGAARADVTRVAETLTAQAGEIAAIGAGPAMLADLD
                     RVQQGWHRVALRQARGRALAAGGILLAVGATTWAVAHTLFVEQRHTAPVAALLVLAPV
                     AVADALTPLAEAARALARARGSATRLAAVLDQQPAVAGALDTPADAGSPPPSATDPGA
                     RIALRDISARWSPERPLALDPLDLDLPVGEHLAIVGPNGSGKSTLLAVLARHLDPETG
                     SYAIGEADALSTDLAATRRHYAVVDDTPHIFASTLRENLRFAAPGGNDAGDPRSAGVA
                     VPDDELRDALRLAGLGEWLAGLPDGLGTRLGTGGRGISGGERARLGLARALVSGRPAI
                     LLDEPVAHLDSATAREVINDLVQASSTRTLVMVSHRDDGRDGFQHTVSLESPTRSV"
     gene            complement(28185..29771)
                     /gene="cydD"
                     /locus_tag="PKU75_03045"
     CDS             complement(28185..29771)
                     /gene="cydD"
                     /locus_tag="PKU75_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HPF79881.1"
                     /translation="MRPFDPRLFRTVPEARGPFVLLAAVGAAGGAATIATAFALTGVV
                     VAIARHTDLSRPLTWLAVLFVARGLLAAVTEYAAAHAGARVSTSLRRHVLTAWTSRPP
                     SDEADPSRRLTLATAGCTSVEPYVARYLPALIAAAVVPLAAIVTLALVDWASALVVVL
                     TLPLLPLFAALIGRTTAEQTQRRWRSLADLAGHFLDVVRGLPTLVNYGRAEKQLTTIA
                     AVSDRHRLATMGTLRLAFMSTGALELLATISVAIVAVLNGVRLAHGSMPLDTAMVAIL
                     LAPEAYWPIRRVGQEFHSAADGVTALEEITSFAESGDPAPPQRCPGPDARIVVDDVTY
                     SYPGAGRPVLTGVSLRAGPGLTAITGASGAGKTTLLELIAGLRRPDAGTITAPACHLV
                     TQRPFLPTGPLSRILALGNGSGAEEQRAAIRAVGLESVVAGLDQGLDSIVGDDGFGLS
                     AGQRARVALARALLAPESVILLDEPTAHLDDASATIAHRAIAQLATRRCVIAVTHRDE
                     LVAEAATHVDLASVAQEVRA"
     gene            complement(29789..30835)
                     /gene="cydB"
                     /locus_tag="PKU75_03050"
     CDS             complement(29789..30835)
                     /gene="cydB"
                     /locus_tag="PKU75_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPF79882.1"
                     /translation="MDLAQLWFLLIAVLWTGYFVLEGFDFGVGMLLPILGKGRDAEDT
                     EKRKRVLLNTIGPVWDGNEVWVLTAGGATFAAFPHWYATLFSGFYLALLLILVALILR
                     NMGLDYRGKRASAAWKAGWDKAIIVCSFLAALLWGVGLTNLVKGVPIDKDMEYTGTLF
                     TLLNPVGLLGGLVFVALFLTHGAYFVALKTDGPIRADARAFATKAGLAAAALAVILLL
                     ALGIQKGSVWSWVTTAIAALALLFSIYANTKGKEGQAFVGTALTIAMAVATYFFLLYP
                     DVMPSSTDPAYSLTIENASSTDYTLKIMTIAAVIFTPIVLLYQGWTYWVFRKRIRVEH
                     IPAAQDPLIHAESR"
     gene            complement(30850..>31042)
                     /locus_tag="PKU75_03055"
     CDS             complement(30850..>31042)
                     /locus_tag="PKU75_03055"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPF79883.1"
                     /translation="GVSPEVSSAEVWISMIVYTLLYAVLAVIEVKLFTDYVKKGAEPF
                     EQPKTPDDMSDDTELQFAY"
BASE COUNT         4751 a        10704 c        10868 g         4719 t
ORIGIN      
        1 aacgccttca tggtgctggc gtgcttgatg gacctgcgcc ggccgcggcc gcggctgctg
       61 cgcgcgctcc cgcccggggc gctggtgggc ttcgcgatgg cgatcaagct gacgccgggg
      121 gtgttcgtca tccactacgt gctcagccgg cggtggcggg aggcgttcac ggccgtcggg
      181 gccgctgctc tggcgtggct gaccgcgttc ttgatcctgc cggaggcgtc gtgggcgttc
      241 tggggcgggg cgttgcagga ccccgcccgg ctcggcccca atatgggcac ctcgaaccag
      301 tccctgcgag gcgtcctgat gcgcatcggc cccgacggag cgctcgggac ggggatctgg
      361 ctggtgtgcg tgctggccgt cggcgcctac ggcttcggga tcgcccagcg ggcacaccgg
      421 gccggcgact cgatcaccga ggtggcggcg gtcgggctga tggcgtgtct gctctcaccc
      481 gtggcgtgga tccaccactt ccactggatg gtcgtggtca tcctggccgt tctgggcgcg
      541 gcgccctggc gggaccggcg ccggctggtg gccgcgggag tgctcctcgt ctggttcacc
      601 tgccggctcc cgtggtgggg gatctcctgg ctcggcaacg gctggtcacc cgacttcgtg
      661 ggccggttgc tccagaacgc cgacctcatc ggcggcctgc tcgccctgtg gctgctgcac
      721 tggtccctcg cgcgggacct cgacgaggcc gccacccgcg agcggggcca ggtttgaccg
      781 gagcggggtc ggcgaacccg gccccgctcg gccccggccc ccgctcgcac tggccagggt
      841 gacggatggc accgaggctc agccgcggac cagctcgacg acggtgagcg ccgacagcag
      901 caggcagacc gaagcgccga cgcggcggat ggtggccagc ttcatccgct tgagcagctg
      961 ccggccgagg gcggcgccga gcgcggaaat ggtggccagc gcgaggaagg cgccgacgcc
     1021 gaccgagacc gggtcctggt acttcgcggc gagcgaggcc gtcagcagct gcgacaaatc
     1081 gccccactcg gcgaggaaga ggatgaggaa agacgccacg acggcgcgga agccggtctc
     1141 ggggacggcg accttggcgg cgaactcctc ctcgacctcg tgctcctcct cgtcggcggc
     1201 gctcgcgccc cggaagagga tgacggcccc gagggcgaac atgcacgccg cgagaatctg
     1261 caccggctgg tggggcagct tggcgagcag gccgccgagc gtcacggcga cgacggtctg
     1321 caccgcgaag gcggaggcga caccgatcca caccaacagc ggccggtagc gcgtcgccag
     1381 cacgagcgtg gcgatgaagg tcttgtcggg gagttcgacg gggaagatca acgcgaacac
     1441 gatcgccgct gtcgcaatgc tgaacgtcat cgcggcccat tctacgggcg tcataccctg
     1501 ccgttgtgac ccgatatggc tacttcggtc cctccgggac cttcacggag atggcgctcc
     1561 tcgactggcc gccggcccgc gacgccgaac gggtcccctt cgcgtcggtc gatgccaccc
     1621 tcgccgcggt gcgcggcggc gatgtcgacg ccgccatggt gccgatcgag aactccgtcg
     1681 agggcggggt gagcgcgacg ctcgacgcgc tcgcgaccgg cagcccgctg atcgtcattg
     1741 ccgaacggct ggtcaagatc cgcttcgtcc tcgccgcgcg ggagggtatg acgctcgaga
     1801 gggtcgcgac gatcggcacc cactcgcatg cctgggcgca ggtgcggggc tgggtgggcg
     1861 cgaacctgcc cgaggtcacc tacgtcccca ccctctcgac cgcctcggcc gccaccgatc
     1921 tcgctcccgg cggcgagggc cgccacgacg cggcgatctg tgcaccggtc gcggcggcgc
     1981 aggcggggct gcaggtcctc gccgacgaca tcggcgacat caagcgcgcg gtgacccgct
     2041 tcgtcctcgt ggcgcgaccc aaccgcatac ccgcccccac cggcgccgac aagaccactg
     2101 tcgtgctcta ccagcgcaag gacatggccg gcggcctgct gcggctgctc gaccagttcg
     2161 ccacccgcgg gatcaacatg acgcggctgg agtcgcggcc gaccaagcac tcgatggggg
     2221 actactgctt ctccgtcgac atcgagggcc acgtcctcga cgagcgggtc ggcgaggcgc
     2281 tgaccggcct gaagcgggtc tgcgccgagg tcgtcttcct cgggtcgtat gcccgcgccg
     2341 acaacggccg cgcccaggtc gccccgttca acggcgacga cgacttccgc gccgccgccc
     2401 gctggctgga cgccatccgc gacctcgtcc agtaacccgc ccccggggct cccgtcgaga
     2461 tgtcgagtcg accccgtcga gatgtcaggc ccccatggca cgcgcaatct ccgggacgca
     2521 tttgacacgc tgggttaggt aaggcttacc ttaagtccgt cggatcgccg gatgaaggac
     2581 cgttcatgct cgtcagcaac gccctggtcg ggctgcgtga aggcctcgaa gcgtccctgg
     2641 tggtcgtcat cctcatcgcg ttcctcgtca agaccgatcg gcgctgggca ctgaaatggg
     2701 tctgggctgg agtggcgacg gctgtcgtcc tctccgttgc cctcgcgggg ttcttgacct
     2761 acggcacccg tcagctgtcg ttcaagaccc aggaactcat cggcgggggt gcctccatcc
     2821 tcgccgtcgc cttcgtgacc gcgatgatct tctggatgcg tcgtgcggcc cgcaccatct
     2881 cgggggagct gaagggctcg atggaccgcg ccctgtccgc gggtggcgtg tgggtggcac
     2941 tggtcggctt cctcggcgtg gggcgcgaag gcctcgagac cgcgatcttc ttctacgcca
     3001 ccgctcaggc agcgggcgcc ggcaccagcc agccactcat cgggtgggtc ctcggcatcc
     3061 tcggcgcagt ggtcatcgga ctgctgatgt atcgcggcgc gatccgcttc gacatcggca
     3121 agttcttccg ctacaccggg atcttcctca tcgtcgtcgc cgccgggatc ctcgcctatg
     3181 gcatccacga cctgcaggag gcggccttcc tgccgggcct gaactcccac gccttcgacg
     3241 tcagctcgat catcgaaccc ggcggctggg tcgccacgct gctcaagggc atcttcaact
     3301 tcaccccagc gacgacgtgg ctgcaggcga tcgcgtggac cgcctatatc gccgtcgtcc
     3361 tcccgctgtt cctgcggcct gtggccgccc ccgcggcacg ccccgtgcca tcggccgcct
     3421 gacccgattc tgttatccca caaggagatc tccgtatgca gtcccgctcg cccctgcgct
     3481 ccgccctcct tctcgcgggg gctgccagcc tcaccctgac cgcctgtggc gggggtgcct
     3541 ccggcgacgc cgggtcgggt tccgccggtg ccggtggccc catcacggtg accgcgaccg
     3601 acaccgaatg caccgtctcg gcgaataccg cccccgccgg caaggtgacc ttcaccgtca
     3661 cgaacaaggg caccaagatc aacgagttct atgtgtatgc cgagggcgac cggatcgtcg
     3721 gcgaggtcga gaacatcact cccggcctgt cgcgcgactt catcgtcgag gtgagcgagc
     3781 cgggctccta cgagacggcg tgcaagccgg ggatggtggg caacgggata cgcagcgctt
     3841 tcaccgtgac cggtgacgcc ccggcggcca agaccgacga ccaagacctg caggccgccg
     3901 tcgcaggcta ctcccgctgg gtgaacagcc agggagatgc attcctcacg cgcacaacgg
     3961 aattcgtcga gctcgtcaag gcgggcaagg tcgaggaggc caaggcgctc tatccggtcg
     4021 cgcgctccta ctgggagcgc atcgagcccg tcgccgagtc cttcggcgat ctcgacccca
     4081 agatcgacgg ccgcgaggat gtcgtcgagg agggcatggc gttcaccggc taccaccggc
     4141 tggagaagga cctgtgggtc gacggactgc agaaggacac ctccgccatc gccgaccagc
     4201 tgctcgccga tgtcaccgaa ctcgtcacca aggccaagtc cgtcgaactc aacccgcttc
     4261 agctcgccaa cgggtccaag gcgctgctcg acgagatcgc gaccggcaag atcaccggcg
     4321 aggaagagcg ctacagccac accgacctgt gggacttcga ggccaatttc gaaggcagca
     4381 agcaggccat cgccgagctg cggccctatc tgaccaagaa ggacgcggcg ctcgtcggca
     4441 agatcgacgc gaccggcaag gccctcggcg acctgctcga gacctatcgc cagggcgacg
     4501 gtttcgtgct ctacaccgac ctgaccgagg acgacgtcaa gaagctgacc caggccctgg
     4561 atgcgttctc cgagaacgtc gctcaggtcg cgggcgtcgt cgcgaaggca tgaccgacac
     4621 gcctgctccc gcccctggcg ccgatggcgc cggggcgagc cggcgcgccg ccgtgggttc
     4681 ggcgctcggc gcggcagccg gggtcgccgc ggccggggcg ctgtatgccg ccaaccgcgc
     4741 cgacgcggcg acgtcgccgg ggcaggcgag cacgccgcat accgcacctc ctcgcggggg
     4801 cgaccccatc gccttccgcg gcggccacca ggcgggcatc accacgccgg ctcaggaccg
     4861 catgcacttc gtcgccctcg acgtgacgac cgagtccgcg gacgagttgc gcgagatgct
     4921 caccgcatgg acgcgtgcgg ccgaacggat gacctacggc gccgaggcag cgccgggcgg
     4981 tgccgtgggg ttggggccgt atggcgtgcc caccgacacg ggtgaggcgc tcgacctgcc
     5041 gccctcgaac ctgacgatca cgatcggtta tgggccaacc cttttcgagc gtttcggcct
     5101 cgaggcacgg cggccggacg ccctggaaga gttgccgggt ttcatgggcg acgacctcga
     5161 tcctgcgacc tcgggcggcg acatctgcat ccaggcctgc gccgacgacc cgcaggtcgc
     5221 cgtgcacgcc gtgcgcaacc tgatccgcct cggctttggc gtcgtgtcgg tgcggtggag
     5281 ccaattgggt ttcggcagaa cgagttcgac gtcatcgagc caggcgacgc cgcggaatct
     5341 gttcgggttc aaggacggca ccgccaacat catggccgag gacaccgcgg cgctcgatga
     5401 gcacgtgtgg gtcgcgccgt ccgatgtgtc cggcgaggcg gcgtggatgg ccggcgggtc
     5461 ctatctcgtc gcgcggcgca tccggatgca catcgaggtc tgggatcgga ccaatctcca
     5521 agaacagcaa gacattttcg gccgcggcaa gctcaagggc gagccgctgg gtggcacgga
     5581 cgagttcgag acgatcgact tcgcggccaa gcgcatggat ggcgagcccg ccatcccgat
     5641 ggtcgcgcac gtgcggctgg cccactccag caacctcggc ggagtgcgga tccttcggag
     5701 gggctacaac ttcacggacg gctccgacgg tcaaggccac ttgaacgccg gcctcttctt
     5761 catcgcgttc atgcgggacg cccacaagca gttcgtcccg atgcagcgtg ccctcgccgc
     5821 caaggacgtg ctcaacgaat acatcgagca caccggctcc gccctcttcg cctgcccgcc
     5881 cggcctcggc ccggacgagg actggggcac ccagctcttc ggtcgctaag tccgacgttt
     5941 gacgagtcag gacccgccaa acgtcggact ttgcgagggg gccctcattc gccctgccgc
     6001 tcgacccagg cgtcgatctc ggcccagcgc gcagacagcc aggactcgat cgcggcgggt
     6061 tcgcgaggga ccgtctcggc ggggtaggtc caggtgtgca cgaggaaggg ccgctcgaac
     6121 gggatcgcgg catagatctc ccgtgccccg gtcagccggc ccatgccggc atgcgcgacg
     6181 atcatgacgt cagcgtcggg tcgcgccgac aaggtcgcga tggcgccttt ggtcctgggc
     6241 ggcaacacat tccgcaaacg ctcggcctgt cgggcccggg cgtgctgacc tgcttcccgc
     6301 agtcgggtga tcaggcggcc gcggcgcgac ggcgtccagt tctccccctc gggaaagagc
     6361 agcagagcat ccttgcgcgc gagcgaatcg gcgaggcggc gcaccccggc cagtcggtcg
     6421 tcacccgctc cggacgcgga ggggacgaag gaggagccca gccgggtgag gatgaggtcg
     6481 atgccggggt cccagcgcaa ggcgtcggcc aggacgatgc ggggcagccg gccgccggtg
     6541 cgcgagagca gccaggcgac cgcgagggag tcggccggtc cggcatggcg ggcaaaggcg
     6601 atcagcggct tgcccgggtc gccgaggtcc atgacgtcgt caagtcgcac ctcgaatccg
     6661 acccacttgc gccccaacgc catcgccttg tccaggacgt cggagagcaa ctgctcgtgg
     6721 tcggcatgcc aggagggcga cgcgccgcgc gggtcggcca gccgcagccg ccagcagccg
     6781 atcagcagtt gcacgtcgag ccagacgacc aggatgagca ggccggtcgc gcggaagagc
     6841 cgcgcgcggc gatccacgac cgacgccagc gcaccggcca cgagaacggg cagccccagg
     6901 atcgtgatcg tgagagccag gagcggccac acgacacgca aggcgcggcg caccagcacc
     6961 ggcggcggct ggatcgaccg catccccatg tcccacaacc cctttcagtc tggctcgacc
     7021 ggatcgccgg ccagataggc ggtgctggcg gcatacgcct gctcgatccg ttcggcgacc
     7081 cggcgcccgc gcgcctggct cagcgacacc gtcggtgccg aggaggtccc gctcggaagc
     7141 acatgcacgg caacggattc ggggatgcgc gtgagttcct cgacgtagcg atgccggcgc
     7201 gagatctcga aggccacggt cgcgacgtcc cacggcgcgc ggggcaccgc gaggggctgt
     7261 tcgacgcggc cgacctggag cacatagacg cgggtggcgc cgagttccaa cgcccgtccg
     7321 accgggatgg agtggacgag accgccgtcg aggtagtggg ccccgtcgat gcgcacaggc
     7381 gggaagaggc ccggcaccga gcacgacgcg agcactgccg gcgcgatcgg ccctgcgtcg
     7441 aaccacgccg cgctgctgtc ctcgatccgc gcggccacgc actggaacgg aacggccagg
     7501 tcctcgaact cgtgcaccgg caggtagcga tcgatgagcg tcgcgagcgg ggcgtgggtg
     7561 agcaggtggg tgacatggcg cgcgatcttg gccgccttgc gcaccggccg ctcccacaac
     7621 acgccctcgc gcaccacctc ctcccacacc gacgccagcc gttcggcgcc ccagacggtc
     7681 gggtcggagg cgatgaaggc gccgttgagg gcgccgatgc tggtgccgac caccaggtcg
     7741 ggtgtgatgc cggcctccgc gagggcgcgc atcatgccga cctgggacgc cccgagcacg
     7801 ccgccgccgc cgaggacgaa ggcggtccgc tcggtcatca ccccagccta gtttcgtccc
     7861 gccggaccgg cgcgcattct cacctggccc ctgccagggg tggggtggcg aatcccgtgc
     7921 agtccacgac gaggtcggtg tcgtcgggag tctgcgacgc gatccggaca ccgagccgct
     7981 ccagtgctgg gaggaggtgc cgcctccctg aatcggagta gctggtgccg accgggatgg
     8041 ggtcggcgag agtgaggcgc aggtcggggc gcgcatacgc gatctcactc gccacctcga
     8101 caccggtcag cccggcgccc gccaccgcga cgcttgcgcc cgggggaaga ccggcgacgg
     8161 cggcgtggtt cttctccgcc cactccaacc cgtctggccc ggcgccgctg ccggtggcga
     8221 cgacgatgtg gtcgcctctg accgtctcgc cggtggccag ttcgacggtg cgcgagcgga
     8281 ttcgggctgc cggggcgatg acgaggcgca cgcgcgggcg gaccgccttt tccagcggga
     8341 tctgggcggc ctccggggtc ctgatgccgg ggatgacctc gtgcagccgg atccggtcca
     8401 cgaagctgtc gcggtcgttg acgagcgtga cctggtggcc tttggcggcg gctcgattgg
     8461 ccgccaacac acccgcgtag cctgccccga cgatgacgat gtgagtgttc ttgtcttcgt
     8521 gagtgttctt gtcttcgtga ctgttcatgc ccatgtgaca gagcaactgc gccgagtgtg
     8581 acatcgccct gcgatcgggc agcggatcag gccgaggtcg ggtcccgaga tcaggcatat
     8641 gtggacctca cccgccgcga ccacctcggg cggcccgggg ccttgttgcg tgatccgtcg
     8701 cgactcagaa aagcgcttgc gcggaactcc cggcgtcgtc accctttggg ggtgagcgag
     8761 ccagcctttc cgatgcgcac cgatcgcctc gtcctgcgct tcgtcgccga cgaggacatc
     8821 gcgccgctga cggcataccg gaacgaccct caggtggcgg cgctccagga ctgggatctc
     8881 ccctattccg aggagaaggc gcgcgcgctc gtggccaagc atgaggggct aaccgatctc
     8941 gtggccgggc gcggtcatca gatcgccatc gtccgggacg gccaactcgt cggggacgtc
     9001 tatgtcggga tcgacgagca cgggggagtc gccgacatcg ggttcagtct gatccccgcc
     9061 gcccaaggcc agggcatcgc tcacgaggcc acgaccgccc tcatcgatca cgtcatcgag
     9121 cggcacggcg tgcaccggat cacagcggag ctgtcgacgc agaacctggc ctcggcccgg
     9181 ctgttggagc ggctcggtct caccttcgaa tccttgacgc gccagtcgtt ctggtggcga
     9241 ggggtctggg acgacaacct ctactactcg atgacggccg acgaacggcg ggcttggcgc
     9301 gaccgcccga ccggaccgcc cgcggatgtc cggctggtcc cgatcaccga cgacaaccgc
     9361 tgggcctatt cgcggctgcg cgtgcatcgc tcgcaggagc gcttcgtggc gagcgtgcag
     9421 gattcgtatg ccgacgcctt cttccacggc gaccgtcacg gcctcccgct catcccgcaa
     9481 ctgcgcggaa tcgaggccga cggggagccg gtcggcttcg tcatgtattc cgaggccacg
     9541 ccttaccttt ggcggttcct gatcgaccgg cgtcaccagg ggcgcgggat cgggcgccgg
     9601 gcgctgagtc agtggatcga cggaatgcgg accaacgggc ataccgagat cgagaccagt
     9661 tgggtggagg gcaagggcag cccggcgccg ttctatcggg cgctcggttt cgagccgaca
     9721 ggcgagttcg acgagggaga aacgctggcc ctgctcaggc tcgggccaac gccacggtga
     9781 acaacgccac gatggggcaa cgcaacggtg aacaacgccg tcgtgaactc gtgcagtccc
     9841 tcgcttcctt cggacgggtg cggcgagacg cgtcaatgcg gattccgggc gagctggacc
     9901 aactatgcag tgtcgttgcg gcggtaggtg atccagccgt agtcggcgcg ttcgccgagt
     9961 ggtggagtgt aggaacccgc gtcggtcggt cggccgcgac cagggtcgtc ggcttcgggc
    10021 ggcgtcgcgt ggatggccca ccccccgcgg gcagcgaacg tgaacggtat gaccgcgttg
    10081 tggctcgccg cgatggtgaa gactccggcg tggtgggcat cgtggtggtg cgggcacagg
    10141 cagatcaggt tgtcgtagtc cgtgcctccg ccgtcctccc cacgctggag gtggtggatc
    10201 tccacgaatc gggtcgcggt gcagccgggg taggcgcagc cgcggtcgcg gtcctgggac
    10261 tcacgtccga cggccgcgtc cgggctgccg gcgacgatac ctgcggccaa tgcgacgtgg
    10321 ccgcgtggac ggacatcgtc gcgattgcgg cggggtctcg gcacaccgtg ccatccggcc
    10381 ggatggcacg gcgatcgccg caggatgcaa tgacgacggg cagtgcgatg tcgggacgtg
    10441 ggccggcctg cgcccaggtt gaccttcccc ttcatcggcg gcgtgcaccg agatcgggcg
    10501 ggctatccgt ggcgccctcg gccccgcagg cgccaagatg agcgcatggc tgctccgctt
    10561 accgtcatcc tgttcggtgc caccggcgat ctgtccaagc gcaagctcat ccccgggatg
    10621 ctccatctct tccagagccg gctgctcgac gacgtgcgcg tcgtcggcac ctcgctcgac
    10681 gacatcagct tggaggagtt ccgcgacctg gccttgactg ccatcaagga gcacagcacc
    10741 cgcagcctca acgacgagga ctggaatgcc ttcgcccaac gcttggacta tgtgccgctc
    10801 tccgcaggcc cggaggccct gcgtgccgcg gtgctgcgct cggaggaact cctgcccggt
    10861 gaccacgagc tgcgggtgca ctatctctcc gtcccgccca aggccgcgct cgcggcggtg
    10921 tcgacgatcg ccgcggcggg gctcgtcgag cgctcgcgca tcatcatgga gaagccgttc
    10981 ggcatcgacc acgcgtcctc ggtcaagctc aacgctcaac tgcacgaggt gttcgacgag
    11041 tcccagatct tccggatcga ccacttcctc ggcaaggaag cggcgcagaa catcctggcc
    11101 ttccgcttcg ccaacgggct cttcgagccg atctggcacc gcaaccacat cgaccacatc
    11161 cagatcgatg tgcccgagac gctcggcctg acccagcggg gcgacttcta cgagtcgacc
    11221 ggcgcctatc gcgacatggt cgtcacccac ctgttccagg tgctcgcctt cacggcgatg
    11281 gagccaccca ccgcgttgga gccagcggcg atcaaccgcg agaagacgaa ggtcttccga
    11341 tccatgctgc cgatcgaacc gtccaatgtc gtgcgcgggc agtatgtcgg ctacaaggac
    11401 gagcccggtg tcaagcccga atccgagacc gagaccttcg tcgccttgaa gtgctatgtc
    11461 gacaactggc gctgggcggg cgtccccttc tatctgcgca ccggcaagcg catggcccag
    11521 ggggcgcgca tcatctccat cgcgttcaag gagccgccgc gctcgatgtt cccggcgggc
    11581 tccggcgtcg gtgaccacgg ccccgatcac ctgaccttcg acctgtccga ccagccgaag
    11641 atgtcgctgt ccttctacgg caaacacccg ggtcccggtt atcgcctcga caagctgtcc
    11701 atgcagttct cgatggagga gaccagctgg gccggggcgg tcctcgaggc ctacgaacgc
    11761 ctcatctacg acgctgcccg cggtgacagc accctgttca cctccgcgac cggggtcgag
    11821 cgcctatggg aggtcagcac cccgctcatc gagaacccgc cgatcgtgcg gccgtacgac
    11881 caagggtcct ggggtccgaa ccagatccac cagctgatcg ccccgttcag ctggcgcctg
    11941 cccttcgaac gcaagtggcg cgacggcacc tgacgactgg atctcgagtt cgaaccgggc
    12001 ggggtggatc cgggaccagc cctcacccgg ccgggccggg ttcgcccaag gctgccacgc
    12061 gcaccaggag ggccttgggc acgatgcgag ccgagagccg tgtcgtctcg ccgatcacgt
    12121 ctccgtccaa ttgcactgcc tgtgccttgt cggtggcgat gacgacctcg gtggccgtat
    12181 gccgttgcag gcgctccgtg cgattcgacc ggtgctgggt cagcacccgc gcggtcacat
    12241 cgacccatcc cggaaaaccc ttgggggaca ggacgacaac gtcgaggaca ccgtcatcga
    12301 tgcgcgcgtc gggcatcagg ttgatgccgc ccgtgatccg tccgcagttg ccgatgagca
    12361 ccgtcgacgc cgtgacactc cgggccggac caccatcgac ggacacgtcg gcgcgaatgc
    12421 gggtgccgag gaagttcttc aaacccgttg ccgcgtatgc cgtccagccc agtttggcct
    12481 tggactcctc gctggtctcg gacatgatcg tggcgtccaa gccgacgccc gccatcacca
    12541 ggaacgcgtg ctccgccgcc gcgtccgccg cctccgtcga gtcgcccgct ccctggccgc
    12601 cggcttcctg ctcgccggga tgaggggctg gggaaggcgg aggagcgagg gcggcatacg
    12661 cgacgtccac cgcccggttc cggccggcgc aggcgacgcg gaacgcctcg ccgatatccg
    12721 tcagcggcaa ctcgagattg cgggcgagca ggttcccggt gccgccgggc agcagaccca
    12781 tcggcgtcga ggtgccggcc agcacgctcg cgacaacgcg cacggtgccg tcgccaccga
    12841 gggagcagat caggtcgacc cccgcatcga gggcgctgcg ggcctgccca tagccgggat
    12901 cttcgcgggt ggtctccagg atcagcggct ggtcccaatc gagttctgcc gcaacggaac
    12961 cgagcgccga ccgcacaggc gtcagcgtgg tgaacttggt tgggttgacg atgacggcgg
    13021 cgcgtttgcg gcgcggcggt gtcgtcgcgg cgccacgaaa ggcgtcgcgg tgcgggcggg
    13081 gtttcgcgtg acgtcccggt cgtccgttga gtgccagcac gatcgcgacg gccaagagcg
    13141 tcacgttcag tgccaccgcg gcagcgagca tcagggcgtt gggcaccatc cgaaactatc
    13201 gtggccgtgc tccggcgtga aatgccctac gccgaacggg ccgacatacg tcaccatgac
    13261 cccatgcccg acccgacgag ccagacgcct cccacccccg cgcccgacgc gaaggactgg
    13321 acgtgggtca tcgagcagcc ctgccccgac tgcggcttcg ccggggccct gatcgcgccc
    13381 ggcgagttcg cgatccgcat cgccgccgcg accgcgccct ggcaggaggt gctccggcgt
    13441 tccgacgtcc gcgaccgccc tgccccgacg acgtggagtc cgctcgaata cagctgccat
    13501 gtccgggacg tctgccgcat cttcgacgag cgcacccggc tgatgctcac cgagatcgac
    13561 cccggattcg ccaactggga tcaggacgcg acagcactcg aggagcgcta ctgggagcag
    13621 gatcccgccc aggtcgcggc cgatctcgcc accgcggcaa cccagttgag ccggacgtat
    13681 gccgccgtcc ccgccgatgc gtgggggcgc accgggcgcc gcagcaatgg gtccgttttc
    13741 acggtgacga ccctggggcg ctacctgctg cacgatctgg tccaccatct ctgggatgtc
    13801 ggtgccgcgc cgctcgccag ggagaagtga tccgtatgcc gcccgcccac ttgtggttca
    13861 gcttcctgat cgcggggctg gtcctgtgcc tgatccccgg gcccagcctg ttgttcacca
    13921 tcggccgggc gttggctgaa ggccggcgca ccgcgctgct gtccgtcctc ggcaacggct
    13981 tgggcatcct gacccagatc ctcgccatcg ccgtcggcct ggggcccctg gtggcgaaga
    14041 gcggggcgct ctacaccacc ctcaaactcg cgggtgcggc atatctggtc tctctcgggg
    14101 tgcagaccat ccggcatcgt cgcgacgccg agtccgcgct cgcgattctc gatgcgcccc
    14161 ggcgtccggc gcaccacgcg gtgcgggacg gattcgtcgt gggcgtgacc aatcccaaga
    14221 ccatcgtctt cttcatcgcc ttgttgccga gcttcgtcga cccggccggt gcacctgtgc
    14281 cgctgcagat ggccgtcctc ggcgtcacct tcttcctgat cggcatcacg tgcgacggcc
    14341 tcgtcgccac cgccgccggc accgcccggc aatggttcgc ccgccagccc aaccggatgc
    14401 gccagctgag cgccgcgggc ggggtgatga tgatcggcct cgcgctcgcg ctggccctga
    14461 ccggccgtcc aggtgaggcc cacgcccgct gacgctcgtg gtaagcatgg ggcatggagc
    14521 cgcccggagc gttgcgcgtc gtcttcgtct cgatgcacac ctcgcccgtc gccgaccctg
    14581 gttccggtga tgtcgggggc atgaacgtcg tggagtccgc cctcgcggac gcgctcgccg
    14641 atcgcggaca ccaggtcgag atgatcaccc ggcgagccga caccgaatcc ccggacatcc
    14701 gcgagttgcg acccggcgtc accctgcggc aggtgcccgc cggaccgcca acgcccttgc
    14761 ccaagagcgt catcgacgac cacatcgacg aattcgcggc tggcctgcgc gctgtggcgc
    14821 ggccggacat cgtccactcc catcactgga tgtccggcgt cgccgccctg gcctgggcac
    14881 gcgagatggg agtcccgcac gtgcagagct tccactcggt ggccgcgcca ccgaaccgcc
    14941 cgctctccga cggagaacca cccgagagcc cgcggcgcgt cgacggcgaa cgggtcgtgg
    15001 cgaggctctc ggacaccatc gtcgccatca gcgtggccga ggcccgcacc gtcgtcgacc
    15061 ggtgcggcgc cgaccccgac cgggtcgtca tcgtgcgtcc cggcgtcgac ctcgacctct
    15121 tccaccccgc cgacacccga acccccgacg ccaaccccac cgaaggcaac ggtgcagcga
    15181 gtgcgggagc cacgggtgac aacggctacg tcatgttcat cgcccgcctg caaccgctca
    15241 agggccccga cctggccctg cgcgccctga gccacatcga acagtggcag cgcccacacc
    15301 tgctcgtcac cggcgacgtc tcggaggact tcgcggagta tgccgcggag cttcacgagt
    15361 tggtccgtcg gctgggtctc tccgacttcg tgaccttcac cggggccgtc gaccggcccc
    15421 gcctggcgca gctgctccgg gacgcagaac tgttgctcgt gccgtcccac tccgagacgt
    15481 tcgggttggt cgccctcgag gcggcggcct ccggcgttcc cgtgctcgcc gccgcggccg
    15541 gcgggctgcg cgaggcggtg gctcaccagg agaccgggca gctcatcgac agccgcgaac
    15601 cggaggactg gggtcgcgcc atgcgccacg tcctgcatac ccccggcctg cgcgcgtcga
    15661 tggggcgggc ggcccgcatc cacgcccgtc gcttcgagtg gcgatggtcg gcggcgcggc
    15721 tcgaggatgt ctacggcgac ctgctcggac gttccacgtg agcctgctcc tggaccttcg
    15781 tgggcagaat gtcgttgtcg cccatgccca tcccgatgac gagactctcg ccacgggggc
    15841 attgctcgcc gagatcgcgt ccgccccggg ggaggtgcac gtcgtcaccg ccacccgcgg
    15901 cgagcgcggt gaactcatgc cggggctacc cggccgcgcc gcggatctgg tgccgggcag
    15961 ttggtcgtat gtcgcctatc gcgagggcga gctggccgcc gcgctctcgg cgctcggcat
    16021 acggcaccat gcctttctcg gtcagccacc ggcgcgtgcg gagggcctgc cgagccggtc
    16081 gtatgccgat tccgggatgc gctgggtgag ccccggtgtc gcggggccgg ctgcggacgt
    16141 gggggcggat gccttcacgg cggccggcgt cggggaagcg gcaggggacc tcgcggcata
    16201 cctgcgagcg gccgacgccg acctcgtgat ctcctacgac gcgggcggcg gttatcacca
    16261 tcccgaccac gtgcgcatgc acgaagtggc gaaaacggct gcgcgagagt gcggactgac
    16321 cttcctcgaa gtcatcccgc cggggcgcga cgacatcccc ggcgacctcg acacgatcga
    16381 gtggaccgat ctgtcgcggc acttgcccac ggtccaggca gcgctgcggg cccacgcctc
    16441 tcaggtgcag gtcgagggcg gggacgtcgt gcacgtcggc ggccagcgcg agccgatcgt
    16501 gacgcgagtc ggactgcgcc gggcctgaat tggtcggcgg tcgcccctgg cccgcagata
    16561 aactcgcttt cgtgatcgat atcaagctcc ttgccgacga ccccgaccgg gtcaaggcca
    16621 gccaggccgc gcgtggggac gaccctgcgc tcgtcgacca ggtcctcgac gccgacgcca
    16681 agcgacgggc ggcgctgacc gagttcgagc agttgcgggc ccggcagaag tccatgggca
    16741 aggagatcgc gtcggcgcaa ggtgaggaaa agcagaagct cctgcacgag accaagtcga
    16801 tcgccgcaga ggtcaagcgg ctacaggcga cggccgacga gatggtcgcc acccgcgacg
    16861 agctgttgat gtcgatcggc aatgtcatca tcgacggcgt gcccgccggc ggtgaggacg
    16921 actatgtggt gcgcgaaacc atcggcaccc caagggattt cgctagagag ggtttcgaac
    16981 ccaaggacca cctcgacctg gcggagggcc tcggtgccat cgacatggcg cgcggcgctc
    17041 gggtctcggg ttcgcgtttc tacttcatga ccgggccggg cgcgcgcctc gaactcggcc
    17101 tgctcaatgc cggcatggcc atggcactcg agcacggctt cgtcccgatg atcaccccga
    17161 ccctggtgct gcccgaggtc atgaaaggtg ccggcttcat cgaccgccac gccgacgagg
    17221 tctaccggct cgaggccgac gacctctatc tgaccggcac ctcggaggtc gccctcgccg
    17281 gataccacac cggcgaggtc atcgacctct cgggcggccc gaagcggtat gccggctggt
    17341 cgacctgtta tcggcgggag gccggcagct acggcaagga cacgcggggg atcatccgcg
    17401 tgcaccagtt caacaagatc gagatgttcg tctactgcga cccggccgag gccgaggccg
    17461 aacacgatcg cctgctcgaa atggagcgtg ccatgctcgc caagatcgag gtgccctacc
    17521 gcatcatcga caccgcggcc ggcgacctcg gccagagtgc ggcgcgcaag ttcgacagcg
    17581 aggcgtggat cccgacccag ggtcgctatc gcgagctgac gtcgacctcg aactgcacca
    17641 ccttccaggc ccgccgtctc ggcgtgaaga tgaaggggga gaagggcaat gaggtcgtcg
    17701 ccaccctcaa cggcaccctc gccacgacgc gcttcctcgt cgcgatcctc gagaaccacc
    17761 agcaacccga cggctcggtc atcgtgcccg aggccttgcg gccgtatgtc ggtctcgacg
    17821 tcctgacccc ggtcgcctga cgcggctgac accgtgacga catcacacct cgtcggcctc
    17881 gacgtcgacg ggacgatcct cactcatgaa ggggagctgc gtgaggccgt ccgcgaagcc
    17941 gtccatgcca cggcagccgc cgggcaccac gtcgtgatct ccaccggccg ctcggtgctg
    18001 ggcaccttgc cggtgctcgc cgacctgggc atcgacagcg ggtatgccgt gtgctccaac
    18061 ggcgccgtca cgatcgcctt ggacccggcg cacccgagcg gctacgagat tctcgaagcg
    18121 gtcaccttcg acccgcggcc ggcactggag ctgctgcgag gcgcttggtc ggatgccgtt
    18181 gtcgcggtgg aggatctcgg cgtcggcttc aaggtcagcg ccccgttccc cgaccggctg
    18241 gagggggtgg tcacggtcgt gccctgggag gagttgatgg ccgatcccgt cactcgtgtc
    18301 accttccaca gccccacggg gacgagcgag gatttcacca aactcgccga acgcatcggc
    18361 ctgcacggcg tcaattacgc ggtcggcttc tccgcctggc tcgacatcaa cccggagggg
    18421 gtgtccaagg ggagtgcgct ggagcagatc cggcgccggc tcgacgtgca gcccatgcac
    18481 accgtgaccg tcggcgacca gcgcaatgac gtcgagatgc tgacatgggc cgcccgtggg
    18541 gtcgccatgg gcaacgcacc tgacgaggtg aaagccatcg ccaaggaggt cgcggcggac
    18601 gtctactccg acggcctggc gcaggtattg cggactttgc ccggagtcgg tccgagccac
    18661 caggaactga tcgccccgtg accggcctcg tcatcagctc gccggccaac ccgcgcatca
    18721 aagcggttgt cgcgctgcgc aatcgccgcg atcgggatcg ggcaggggtg acgcgtgttg
    18781 agggccggga agaactcggc ctcgcgctcg cggccggcgc ccgaccccgc acgctgttct
    18841 attgcccgga gctgatgaac gccaactact cgacggctgc gcagggcccc ggcatggatc
    18901 tggtggcgca ggtccgcgcg ctcggcaccg agacgatcga gtgcacccgg gcggtcttcg
    18961 agaaggcctc gtatcgcgaa gggccggacg ggtttctcgc ggtcatcgac gcacccgggc
    19021 gatccttgac cgacctcgac gtgccggacg gttcgctgat gctcatcgcg cagggcatcg
    19081 agaagccggg caatctcggc gccatgctgc gcactgccga tgccgccggc gtggccgcgg
    19141 tcatcgccgc cgatcccggc accgactggg gcaatcccaa tgtcatccgc tccagcaagg
    19201 gcgcggtctt cgccgtcccc gtggcatcgg cgacgaccgc cgagacgctg gcctggttgg
    19261 gcgagcgcgg ttatgcgctg gtggcgacga cgccggacac cgatctgcgg catacggatt
    19321 cggacctgac cggggcggtc gccatcgccg tcggcaccga gaaatacggc ctcgacgagg
    19381 agatgttcga cgcggcaacg gtcaaggtgc gcatcccgat ggccggccgg atcgactcgc
    19441 tcaacgcggc caccgccgcc gcgatcgtgc tgttcgaggc agttcgccag cggctgagcg
    19501 tcaccagaac gacgcaggac ccggagagca cctggggtga atcgtggggt ccctgacgcg
    19561 gtccccgcga agtatcggcg cgacgcagga gcggagaact ttggtgggcg tttctagacc
    19621 gtgacgtcgc cgtcgggtgt gttgaaggtg accgacatca ggcccggagt gccgtggggc
    19681 gcaacgaaat cgaagtggat gaccgtgctc gtggtctcgg ccggcaggcc gagccagtcc
    19741 ttgacccggt cggggtcgcc ggcgatctgc atcgacttca tcgtgacctg ggtgtgcgct
    19801 tcgtcggagg ggtgggggga gtccgcgtcc cattcgacga agaagggcag ctggggatct
    19861 gactgcaggc cccggacgcc gagctggcgc cacttgaact cgcgcccgtc gggcatccgg
    19921 cggttgccgt cgacggccga gcgacccagg cgctcctcca ggtcggagag cgcgtcgacg
    19981 cgcacgaccc atccgagcca acccccgccg atttcgctgc gctggcgcac cgcctgcccg
    20041 aagggtgcct tgtcggccga cgggtgatcg agcacctcga cgacctcgac atagcgttcg
    20101 tgggccaacg gcaggatgat gttgcgcgtc ccgaaccgcg ggtggacgcc cccttcgacc
    20161 ggggtgaccc ccagcttcgc ggccagccgc tcggcggtcg cgtggagacc atccggctcg
    20221 gcggcatacg acacgtgatc cactcgcata cggccaatcg ttccacacct aatggggtgc
    20281 tcttgaccac gcccccgctc atgcgctagc accgcatcgg ctggtgagac aacttgaacg
    20341 gtcaaacgtg cacctgctgg aatgcgtgct ctcccaacgg tctttcgtgc cggcgtcagc
    20401 gaccgaaacg gcgcagccgc aggctgttgg tgaccaccag gacgctcgac atcgccatcg
    20461 ctgcgccggc gatcatcggg ttgagcaggc cggccatcgc caagggaatc gcggcggtgt
    20521 tgtatccgaa ggcccatgcg aggttctgct tgatgatgcg caatgtcgcg cgcgacagcc
    20581 cgatgccctc ggcgaccgat ccgacctccg agcgcatgag caccaggtcg gcactggcgg
    20641 ccgcgacatc ggtgcccgat cccatcgcga tgccgagatc ggcctgcgcg agcgccgcgg
    20701 cgtcgttgac cccatcgccg accatggcga cggtgcggcc gtcctgctgc agcctctcga
    20761 tcatcgcgtg cttgtcctcg gggcgcactt cggcgatgac ccgctcggca ggaatcccga
    20821 cctgttgcgc gacggcgcgc gctgtgccct cggcatcgcc cgtcagcagg taggccgtca
    20881 ggcccaggtc ctcgagctcg cgcaccgcct cctcggcgtc gggccgcagg gcatcggcga
    20941 cggtcaggct ggcgtgggcg acaccgttcc accccaccca tacggtcgtg ccctcggggt
    21001 gggcgggcag atcggcgggc atcttggtga agagacgtga gcggccgacg atgacctcct
    21061 gccccgcgat gacggccttc gcgccgtcgc ccgcaagggc gtggaagtcg ctcgcccgcg
    21121 ggacctgcag gttgcgcgcc gtggcggcgg acgtgatcgc gtgggcgatc gggtgctcac
    21181 tcccggcctc cacggcggcg gcggcacgca gcgcctgctc cttggtgaga ttgccgtgcg
    21241 gtgtcacgtc gatgagcgtc ggcttgccct gggtgagggt tccggtcttg tcgagcacga
    21301 cggtgtcgac gcggcgcgtg tcctccagga cctgcgggcc cttgatcagg atgcccaact
    21361 gggcgccccg gccggtgccg acgaggatgg cggtgggcgt cgccagcccg agcgcgcagg
    21421 ggcacgcgat gatgagcacg gcgatggcgg cggccagcgc cttggtggcg gacccgcttc
    21481 cgatccacca gccgacgaag gtcagcagcg cgagcccgag cacgaccggg acgaagatcg
    21541 ccgagatccg gtcggccagg cgttcgacac ccgccttgcc ggtctgggcg gtctcgacga
    21601 gccgggcgat cccggcaagg gtggtgtccg cgccgacgcg ggtcgcacgc acgagcagcc
    21661 ggccggcacc gttgatggtg ccgccggtga cttccgaccc cgctgccaca tccaccggca
    21721 tggactcacc ggtgaccagg gcggcgtcga tcgtgccccg gccgttcagg accacgccgt
    21781 cggttgcgac cttctcgccg ggccggacga cgaactgctc gccgacgccg agctggtcga
    21841 tcgggatgcg cacctcctcg accacgccgg tgacggcgtt gcgccgttcg acggcgacat
    21901 ccttggcacc gagatcgagc agggtggtca gcgcggactt gccctgcgcc ttggcgcgcg
    21961 cctccagcca ccgaccgagg aggaggaagg tggtcacgac ggcggcggtc tcgaagtaga
    22021 ggtgaactcc cttgtctgcc atacccatat cagtcatggc ctgaccggtg agcacaccca
    22081 cggccgacca cagatacgcc gcgagcaccc cgatcgagac gagagtgtcc atcgtcgagg
    22141 ctcggtggcg ggcgttgatc gctgccgcac gatggaacgg ccaggcggcc cagagcacga
    22201 ctggcgtggt caggatgaac tgccaccatg cgttgccggg cacatcaccg agggccggca
    22261 ccatggccag caagacgacg gggagggtga aaatcgcgct gatcagcgcc cgccgcttga
    22321 ggacggcgtg cggtgcaacg tcgtggtcga ggtggtcgac cgcccggtcg tgcacgccat
    22381 cgtggtccat ggtgttgtgg tccatggcgt tgtggtccag agcgtcgtgt gacgtcgcgc
    22441 cggcgggtcc cgccgtatgc cgtgtgctcc cgccccggct cggctgccgg ggcgcgatga
    22501 tctcagcgcc gtagccggtg gctcggacgg tgccgaggat ctcgtcgatc gtgtgcgggg
    22561 cggcgaagcg cacgtgcgcc ttctcggtgg cgaggttgac gctcgcctcg acgccctcga
    22621 gtttgccgag cttgcgctcg atccgggccg agcaggaggc gcacgtcatc ccggtgatcg
    22681 cgagatcgac ctcttcggtt tcggctgggg cagtgtgggt ttcagtcatt gtcatcaact
    22741 cgatgccgtg tctgtgattc gattcaagtc agctgcgcac caggcgcgcg atggccgcgg
    22801 ccgcctcggt gaccttggcg tcggcggctt cgtcggattc gcgggcggcg ttgacgacac
    22861 agtgcccgat gtgatcctcc agcagcccga ggctgaccgc ctggagggcc cgagtgactg
    22921 cggagacctg ggtgaggatg tcgatgcaat atgtgtcgtc ctcgaccatg cgggcgatgc
    22981 cgcggacctg cccctcgatg cgcttcaggc gtcgcaggta gtcgtccttg tcgccgctgt
    23041 acccggccat gctgcctccc gacagttggt tcatgcctct gacaagagca tacccccatg
    23101 gggtattcct gcggacatct cgaggttcct cgggaggcgg gtcactcgcc caggtggtcc
    23161 acgagggtcg cggcgaggcc gacatatgac gcgggcgtca ggtcccgtag tcgcgccgcg
    23221 acgtcgtccg gcaggccgag gccgccgatg aactcgcgca gatccgcctg tccgattcgc
    23281 cggccccggg tcagctcctt cagtcgttcg tagggattgg acatccccgg aaccccttgt
    23341 gccgcaaggg cgcgcatcgc cgactggatg ggttcggcaa gcacctccca gttggcgtcg
    23401 aggtcggcgg ccatcgcctc ggggttggca tcgaggccgg ccaggccgcg gctgacgttg
    23461 tccagcgcga gcaacgagtg gcccatcgcc gtgccgatat tgcgctgcat cgaggagtcg
    23521 gtcaggtccc gctgctgacg ggaggtgacg agcgtcgagg ccagcacgtc gagcagcgca
    23581 ttgctcacct cgaggttcgc ctcggcgttc tcgaagcgaa tcgggttgac cttgtgcggc
    23641 atcgtgctcg acccgatcgt gccctgaccg cgcacctgcg agaagtagcc gaaggagata
    23701 taggtccaca cgtcggtgca caggttgtgc aggatccggt tgaatcgcgc caggtcggca
    23761 tacagctccg cctgccagtc gtggctctcg atctgcgtgg tgagcggatt ccacgtcaag
    23821 ccgagggact cgacgaactc gcgactgatc gtctcccagt ccgcgtccgg caccgccgcc
    23881 aggtgcgcgc cgtaggtccc ggtcgcgccg ttgaacttgc cgaggaactc cgccgcgtcg
    23941 atcctgcgca cctgacggcg cagccgccag gccaggacag cgagctcttt gcccatggtc
    24001 gtcggggtgg cgggttggcc gtgggtatgg gcgagcatcg gcgtctcgcg caactcacgg
    24061 gccatcgcct cgagctggtc gagcagggcc tgagccttgg ggagccacac cgcacgcgtc
    24121 gccccgcgga tcatgagcgc ataggagagg ttgttgacat cctcggacgt gcagccgaag
    24181 tggatgagtt cactcatcgt ctccgcctgt gccggggcga tcgcggtgag gcgctccttg
    24241 aggaagtact cgatcgcctt gacgtcgtgc tgggtgaccc gctcgatctc ggcgagtgcc
    24301 tcgatgtctg ctgcgccgaa ctcgtcgacg atcgcccgaa ggcggctctt ctcgtcctcg
    24361 ttgagcgtgc ccgagccggg tatgacgccg cgctcggtga ggaagatcag ccacgcgatc
    24421 tcgacccgca agcgctcccg gttgagggcg ggctcggaga ggtggtcgat cagcggtgcg
    24481 acggcactgc ggtagcggcc atccaatgcg cccagggcgg cgtcggtgtc ggcgagactc
    24541 ggcatgccga caatcctgcc agccacccac tccgatgccg agcgcgtcca cgggagaatg
    24601 gctccatggg tcaccctgtt cgcctgctgt ccgcgtccgt cgacatcgcc gcgcccccgg
    24661 caaccgtatg gcgcatcgtc accaacgtgc gccggaccgg tgagtggtcg cccgagtgcc
    24721 gaaaggtatt ggcgcccaag acaatcggtg ccgggtcacg tttcgtcggg atcaaccagc
    24781 gcaggctcat gctctggccc accaacgcca aggtggtgcg cttcactccc gagcgcgaac
    24841 tggcataccg catcgccgag aacggctcga cctggagcta ctctctggaa ccgatagacc
    24901 acggcacccg actgaccgag gcgcgcgagg cagccgacgg catcacgggc ttcgcccacg
    24961 tgttcaccaa gctgttcctg ggcggtgagg cgcacaccag cgagctcgac gacggcatcc
    25021 gcgctagtct cgatcgcatc aaggtgatcg ccgagcgcga gcggccctga acggtccggc
    25081 gccgtcacac ggtccgggag cgctgtgcca gttcggcttt gacgtgctcg acgatgccgg
    25141 gcacggcggc ttgcatctcc tcgtaggtgc ggtcgaacgc cttgtcgttc caggcatacc
    25201 agggatcgtc gacatcgagt tcgccggcct cgacggcggc cgggtcgaag ctgcggatca
    25261 accggatgtg ctccggcgac ccgcccgcga ggcgctgcaa cgcccgcagg tggccggaat
    25321 cggcagccag gaccaggtcg cgctcagcga gccagtcctt ctcgatccgc cgtgcccggt
    25381 gggcggctcg gccccagtcg tcgtggccgt tgcgctgcaa cacatccagg gtccggtagt
    25441 cggcggcatt gcccgactcc tcggatgacg tgccggccga gtcgacggtc acggcgtccg
    25501 acagcccctc gcggtcgaag gcctcgcgga gcaggaactc gagcatgggg gagcggcaga
    25561 tgttgccggt gcagatgacg cagacgcggt aggtcacgac cccattgtcg gtcaccagac
    25621 ggacctcacc tgacgggtga ccggccggcg agggggcgga tcgtgaatct gcggtcgcgg
    25681 atcgcgtcga tcaggtaggg cagcgcggcc acacttccgg agcggtcgat gcgttgcgcg
    25741 ttcggtccgt cgagggcgcc gccgtcgtgg gtgaggatga ccgatccggg gcgcatgcgg
    25801 ccggcgattc gttccgccgc accgcgcggg ccgtggtcgc gcacaaaggc ttcgacacag
    25861 tcggtccaga acgtcgaccg cagcccgatg tcgtcgacgc tcgcgcgcac cgcgtctgac
    25921 gtccagccgc gcggcgaacg gaacaggccg gtcgtggcca ccgcgacgct cgcgaaagag
    25981 cgctcgcacg cggtgatctg gctgtgcacc tgctgctgcg gcaggccatc gagccacagg
    26041 tgatcctggg tgtggttgcc gatgacatgc ccgcgcgacg cgatctcgtg aaccaggtgg
    26101 gggtgggcga gggcgttgcg tccgatgagg aagaacgtgg cgctgacgcc ggcttgatca
    26161 agccggtcga ggacggcggg ggtgtatgcc ggatcggggc cgtcgtcgaa ggtcaagaag
    26221 acctcgcgct cgtcggtctc gacgcgtccg atggcgccgg gttcgacccg caagcgtcgc
    26281 gcgagggagt ccgtatgcgg acccgccgac tgccggtggg cggcccacgc gcctccgccg
    26341 gtcgccgtga gcaggcaggc gccggcaccg ccgaggaagt gcctccgggt gatccccacg
    26401 ctgccctccc ctgcctcgaa cgacccagga atacccggta tgcggcgatc gtatgtccgg
    26461 atcgccgcga cgccaagcgt cgtgactgat tcgtcacacc gagcgcgtcg gcgactccag
    26521 cgagacggtg tgctggaatc cgtcgcgacc gtcatcgcga tggctgacca tcaccagcgt
    26581 ccgggttgac gaagcctgca ccaggtcgtt gatcacctct cgtgctgtcg ccgaatccag
    26641 gtgtgccaca ggctcatcca gcaagatggc cggccgtccg gagacgagcg cccgggccag
    26701 gccgagtcgc gctcgctccc cgcccgatat gccccgaccg cccgtgccga gccgggtgcc
    26761 caggccgtcc gggaggccgg cgagccattc gccgaggccg gcgagccgca gcgcgtcgcg
    26821 cagctcgtcg tcgggcacgg cgaccccggc cgaacgcggg tctcccgcgt cgtttccgcc
    26881 cggagcggcg aagcgcaggt tctcgcgcag cgtcgaggcg aagatgtgcg gtgtgtcgtc
    26941 gacgaccgcg tagtgccgcc gggtcgccgc gaggtcggtg ctcagggcgt cggcctcccc
    27001 gatggcatag ctgccggttt ccgggtcgag gtggcgggct agcacggcga gcagtgtcga
    27061 cttgccgctg ccgttgggcc cgacgatcgc gagatgctcg cccacgggca ggtccaggtc
    27121 gaggggatcg agggcgaggg ggcgctccgg tgaccatcga gccgagatgt cccgcagggc
    27181 gattcgggca ccggggtccg tggcggacgg gggaggggag cccgcgtcgg ccggggtgtc
    27241 gagtgcgccg gccaccgccg gctgctggtc gaggacggcc gcgaggcggg tggcgctgcc
    27301 gcgggcgcgg gcgagtgccc gggccgcttc ggcgagcggg gtcagggcgt ccgcgacggc
    27361 caccggcgca agcacgagca gggccgcgac gggggcggta tgccgttgct ccacgaagag
    27421 cgtgtgcgcc accgcccacg tggtggcccc gacggccagc aggatcccgc ccgcggcgag
    27481 ggcccgccca cgggcctgcc gcagggctac ccggtgccac ccctgctgca cccggtcgag
    27541 gtcggccagc atggccgggc cggccccgat cgcggcgatc tcaccggcct gcgccgtgag
    27601 ggtttcggcg acgcgggtta cgtcggcgcg ggccgccccc agcgccgcat tgcccgctga
    27661 ctcgacgcgg aaagcgatga gggacaacgc cgtcaccacc agaccgagca ccgcgatgca
    27721 cagtccgacg cgcgcatcga aggccgtcgt gaccgctgtc gtgatccccc aggcgatgag
    27781 tgccgacagc cccggcacgg tcacgcggat ctgggcgccg acgacgtcgt cgaggtcgtc
    27841 cacgaccccg gtgagcaggt cagcccggcc gcgccggccg aggcgcgccg gcgtgagagg
    27901 gatcagcgcg gcatacgtgt cgacacgccg ctgggcgaga tcccgcagcg cggcgtcatg
    27961 ggtgatcagc cgctcccagt agcggaaggc ggggcgcgcc ataccgaacg cccggacccc
    28021 gacgatggcg accatcaggg tgaggatgac cgggcgctcg ctggccttga cgatgagcca
    28081 acccgaggtc gcggtgaggg cgacaccact ggcgagggcg aggccgccga tgaccccggc
    28141 ggccacgagc tgccgagtga gccggacggt gcgcagatca ggtgtcatgc gcgcacctcc
    28201 tgcgcgaccg acgcgaggtc gacgtgggtg gcggcctccg cgaccagctc gtcgcggtgc
    28261 gtgacggcga tgacgcagcg ccgcgttgcc agctgcgcga tggcgcggtg cgcgatcgtc
    28321 gcgctcgcgt cgtcgaggtg cgcggtcggc tcgtcgagga ggatgacgct ctcgggggcg
    28381 agcagcgcgc gggccagcgc cacccgggcg cgctgccctg cggacagacc gaaaccgtcg
    28441 tcgccgacga tgctgtcgag gccctggtcg agaccggcca ccacgctctc caggccgacg
    28501 gcccggatgg ccgcccgctg ctcctcggcc ccggatccgt tgcccagggc gaggattcgt
    28561 gacagcggcc cggtcggcag gaaggggcgc tgcgtgacca gatgacacgc cggcgcggtg
    28621 atcgtgcccg cgtcgggccg gcggagaccg gcgatgagtt cgaggagggt cgtcttgccg
    28681 gcgcccgacg ctccggtgat cgcggtcagc ccgggcccgg cccgcaacga caccccggtc
    28741 aacacgggcc gccccgcccc cgggtaggag taggtgacgt cgtcgacgac gatgcgtgcg
    28801 tccggtccgg ggcagcgctg cggcggcgca gggtcgccgc tctcggcgaa gctggtgatc
    28861 tcctcgaggg cggtgacacc gtcggccgcg ctgtggaact cctgcccgac gcggcggatc
    28921 ggccaatagg cttccggcgc caggaggatc gccaccatcg cggtgtccag cggcatactg
    28981 ccgtgcgcca gccgcacccc gttgagcacg gccacgatcg cgacggagat ggtggcgagc
    29041 agttcgaggg cgccggtcga catgaacgcg agacgcaatg tgcccatcgt ggccagccga
    29101 tggcggtcgc tgaccgccgc gatggtcgtc agttgcttct cggcgcggcc gtagttgacc
    29161 agtgtcggca ggccgcggac gacgtcgagg aagtgccccg cgagatccgc gagggagcgc
    29221 cagcgccgct gcgtctgctc tgcggtcgtg cgtccgatga gggcggcgaa gagcggcagc
    29281 agcggcaggg tgagcacgac gaccagcgca ctggcccagt ccaccaaagc cagcgtcacg
    29341 atcgccgcca ggggcacgac ggcggcggcg atcagcgcgg ggaggtagcg ggcgacatac
    29401 ggttcgacac tcgtgcagcc ggcggtcgcg agtgtcagcc gccggctcgg gtccgcctcg
    29461 tccgacggag gacgcgacgt ccaggcggtg agcacgtgac gccgcagcga cgtcgagacc
    29521 cgggcgcccg catgcgcggc ggcatactcc gtcaccgcgg cgaggaggcc acgcgcgacg
    29581 aagagcaccg cgagccacgt cagcggacgc gaaaggtccg tatgacgtgc gatcgcgacc
    29641 accacgccgg tcagggcgaa ggcggtcgcg atcgtcgctg cgccgccggc tgccccgacc
    29701 gccgcgagga gcacgaaggg gccgcgtgcc tcggggacgg tgcggaacag ccgggggtcg
    29761 aaaggcctca cgcgttaatg gtcggccatc aacgcgattc ggcgtgaatc agcgggtcct
    29821 gcgccgccgg gatgtgctcg acacggatgc gcttgcggaa gacccagtag gtccagccct
    29881 gatagagcag gacgatcggg gtgaagatca cggcggcgat cgtcatgatc ttcagcgtgt
    29941 agtcggtgct ggaggcgttc tcgatcgtca ggctgtatgc cgggtcggtc gaactcggca
    30001 tcacgtcggg gtagaggagg aagaagtagg tcgcgacggc catcgcgatc gtcagggcgg
    30061 tgccgacgaa ggcctgcccc tccttgccct tggtgttggc gtagatcgag aacagcagtg
    30121 ccagagcggc aatcgccgtc gtcacccagg accagaccga acccttctgg atgcccaggg
    30181 caagcagcag tatgacggcc agggccgcgg cggcgaggcc cgccttggtg gcgaaggcgc
    30241 gggcgtccgc ccggatcggg ccgtcggtct tgagggcgac gaagtaggca ccgtgggtga
    30301 ggaagagggc gacgaacacc agaccaccga gcagcccgac cgggttgagc agggtgaaga
    30361 gggtgccggt gtattccatg tccttgtcga tcggcacgcc cttgaccagg ttggtcaggc
    30421 cgacacccca cagcagcgcg gcgaggaagg aacagacgat gatcgccttg tcccagcccg
    30481 ccttccacgc ggccgaggcc cgtttgccgc ggtagtccag gcccatattg cgcaggatga
    30541 gagcgacgag gatgaggagc agggcgaggt agaagccgct gaacagcgtt gcgtaccagt
    30601 gcgggaaggc cgcgaaggtg gcaccgccgg cggtgagcac ccacacctcg ttgccgtccc
    30661 agaccgggcc gatcgtgttg agcagcacac gcttgcgctt ctcggtgtcc tcggcgtcgc
    30721 ggcccttgcc gaggatcggc aggagcatcc cgacgccgaa gtcgaagccc tcgaggacga
    30781 agtagccggt ccacaggacg gcgatgagaa ggaaccagag ctgtgcgaga tccatgactg
    30841 tctcctttct cagtacgcga actgcagttc ggtgtcgtcg gacatgtcgt cgggtgtctt
    30901 gggctgctcg aagggttcgg cgcccttctt gacgtagtcg gtgaacagct tgacctcgat
    30961 gacggcgagg acggcataga gcagggtgta gacgatcatc gagatccaca cctcggccga
    31021 ggagacctcg ggggagacgc cg
//
LOCUS       DAOPJG010000016        28713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1744_length_28713_cov_7.316317, whole genome shotgun sequence.
ACCESSION   DAOPJG010000016 DAOPJG010000000
VERSION     DAOPJG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 28713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28713
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1744_length_28713_cov_7.316317"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1355)
                     /gene="glgA"
                     /locus_tag="PKU75_03060"
     CDS             complement(162..1355)
                     /gene="glgA"
                     /locus_tag="PKU75_03060"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HPF79884.1"
                     /translation="MRVDILSKEYPPAIYGGAGVHVAELVRALRARGDMTVQVRCFGE
                     PRDEADTTAYPDLAELASANAAIQTMGVDLSMVAGTEGADLVHSHTWYANFGGHVASL
                     LGGMPHVITAHSLEPLRPWKAEQLGGGYRVSSWVEKTAYEAAAAVIAVSAGMREDILR
                     SYPSIDPEKVKVVHNGIDSQLWQPNPDLDTVRRHGVDPDKKSVIFVGRITRQKGLPYL
                     LRAAANLPPDVQLVLLAGAPDTPEIMAEVEGLMADLREQRGDGVIWVPEMLPRAQVTA
                     MLSAATVFACPSVYEPLGIVNLEAMACELPVVATATGGIPEVVVDGETGWLVPIEQVQ
                     DGTGTPTDPDKFVADLSAALNEAVSDPDRARQFGLAGRARAVESFSWASIGDRTLEVY
                     RSILG"
     gene            1469..2719
                     /gene="glgC"
                     /locus_tag="PKU75_03065"
     CDS             1469..2719
                     /gene="glgC"
                     /locus_tag="PKU75_03065"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPF79885.1"
                     /translation="MAPRKEAKVLVIVLAGGEGKRLMPLTADRAKPAVPFGGIYRLID
                     FALSNVVNSGYRQVVVLTQYKSHSLDRHITKTWRMSTMLGGYVAAIPAQQRLGKSWYV
                     GSADAIYQSLNTIRDEKPDIVAVLGADHVYRMDFSQMVDQHRETGAGITVSAIRQPIE
                     LADQFGVIEVDAKDTTKIARFREKPTDAVGLPDAPHEVLASMGNYVFDADVLVDAVTR
                     DAEDENSTHDMGGDIVPSFVDQDRAYVYDFKNNAVDGATERDRGYWRDVGTLDSYFDA
                     NMDLVNINPVFNLYNYDWPLLTSYGALPPAKFVHGHHNRFGEALNSIVSPGSIISGAR
                     VTGSVVGPKVRVHSYSEVADSVIMDNVTINRSCQIRRTIIDKDVQIPEGTTIGFDLEH
                     DKARGFTVTESGLVVIGKGQTVTP"
     gene            2716..3387
                     /gene="serB"
                     /locus_tag="PKU75_03070"
     CDS             2716..3387
                     /gene="serB"
                     /locus_tag="PKU75_03070"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HPF79886.1"
                     /translation="MTDPFAALAGYPRLLIVSDVDSTFIGQEVIELVARRAGTEEEVA
                     AVTEAAMRGELDFAASLRARVATLAGLPARVLDEVRADVTLTPGVHELLAWAAEEGHA
                     VALVSGGFHEIVDPIAHSLGIRYVRANRLAVADGRFTGEVSGPIIDAAGKAVALRQFA
                     AREDIPLGATIAIGDGANDLDMMALAGLSVAFAAKPVVAQSADIALPGPRIDDLVPLL
                     RLARG"
     gene            3481..4347
                     /locus_tag="PKU75_03075"
     CDS             3481..4347
                     /locus_tag="PKU75_03075"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HPF79887.1"
                     /translation="MPDRAAPATKLLIIGGAEDRVGKAKILKRFVKLAGGKSARIVII
                     PTASSFQDEVVTAYTEVFTRHGAESCAVVNPRTRIEADDAALARVIDEATGVFMSGGN
                     QLKLAQVFAGTAAGDAILRAHQRGAVVGGTSAGASIQSEFMISLGDEGITPRQRSTQL
                     THGLGLLSGVIIDQHFAQRQRYGRLMSIVASSPDLIGIGIDEDTAIEVTDSREFTVLG
                     RGGAYVLDCRRALTDAPEARTGAPLLVSGAVVHALPAGSTFDIVKLMLVDFVEQHPDL
                     GVSVTTAASSRP"
     gene            4373..7165
                     /gene="cphA"
                     /locus_tag="PKU75_03080"
     CDS             4373..7165
                     /gene="cphA"
                     /locus_tag="PKU75_03080"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPF79888.1"
                     /translation="MSIDRPAPAGPPAPDLRIIEERVYRGGNIWSYRPAVHLVVDLGV
                     LEGYPTNTIPGFTDGLLELLPRVGGHTCSRGHEGGFGERLREGTWVGHVAEHVALQLQ
                     QEAGHDERRGKTRMVKDRPGVYNVVYGYTDEGVGLSAGRLAVRLINHLIEPEDEFDFA
                     EELELFLRRAERTAFGPSTAAILEEAASRDIPFIRLNSASLVQLGQGKYAQRIRATMT
                     SHTGALAVDIASDKDMTIKLLGSAGLPVPKQETCRTEDGAVEAARRVGYPVVVKPLDG
                     NHGRGVGLNLDNPDDVRAAFAAAKEQSRNGWVIVESFITGKDYRCLIVGGRMRAIAER
                     VPAHVIGDGTNTIEQLVAITNADPRRGVGHEKVLTRIAVDDAAIERVKDQGYAMDDVP
                     PEGTMVKLALTGNMSTGGISIDRTFDAHPDNVEIAEEAARVIGLDVAGIDFICPDITA
                     PVRETGGAICEVNAAPGFRMHTHPTVGEPQFIAKPVVDLLFPPGAPSRVPIVAVTGTN
                     GKTTTSRMIAHIFKETGKKVGMTSTDGIVIDERLVIKSDASGPRSARMVLQNPRVEVA
                     VMEVARGGILREGLGYDRNDIAVVTNIAPDHLGMKGIDTLEQLADVKAVIVEAVPRDG
                     FAILNADDPHVRRMRNRCSGNIVWYSLKPEDSKERAFIDARLRRGGRAVVLDRTERGD
                     MIVIKHGSRSMQLAWTHLLPATFGGTAMFNVANAMAAAGAAFAAGIGLHEIRQGLRTF
                     TTSYYLSPGRMNLINVRGMEVIVDYCHNAPGMEVLGQFVDRYADRKAATSEWGKISRI
                     GMIATAGDRRDEDLRELGRVAARYFDVLVVREDQRLRGRQPGEAAELIREGIREADAA
                     GGVRVRAAETVLDEVDAVRHCVARANPGDIVVLCVDNHAKVVAELETITHSAQAGSSG
                     DEPGDPDFDARTYPAG"
     gene            complement(7162..9354)
                     /locus_tag="PKU75_03085"
     CDS             complement(7162..9354)
                     /locus_tag="PKU75_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF79889.1"
                     /translation="MSEPVVATEVRLLEGPNLYFTRPAVKVMLSLPGYLDAPLAQVQA
                     VADGLGMRARVGAPGSVQRQAFLGRVAKHLVRALARAAGSQRVGVRVRAGAGPADVVV
                     AAVWRRRARARILGESLAPLLSQLLSGADAEPLIAGIGAQMRETAPGSAATALSPKVP
                     VASITGTNGKTTTTRMLAHMAMTAGKKTAWSSTDGVVVMGEVMEPGDYSGPAGARGVL
                     ETPGLEIGVLETARGGLLLKGMGVTHNDVSVVTNVSADHLGMQGVDTLDQLAEVKAIV
                     TTVTRPDGWTVLNGEDPRVWAMHAHSPGRPWAFALDPTTPALREAIDRKGRGLTVIDG
                     DITILLPGAVTQKVLPLTEIPATLAGLSQHNTANVLAATAAGLGLGLPMEAVVEGLRS
                     FNPDDRLNPGRMNIYSLPAPDGEASVILDLAHNEAGLVALLDVARGLAAPGGRVFLAL
                     GTAGDRTDEIMEALGQIAGQRADQVVIAHKDHYLRGRSTHDFEVLFGAGLARSGVGDV
                     AAYPSELVALQALSEHARAGDVIAVMTHEQRAEMVAWLAERGARADAPEDIRRKVKLA
                     QGKHGREEDFENLWDMTDASARIAAAAALHRALPGDARAAYEYAGTLDAAGREADAVP
                     LYEEALDSGLPEPHRHRARIQLASSLRNLGRPGEAVTLLDALVAERPESAAAIAFRAL
                     ACLDAGQAETGVADLIDALLARATDDDSFAYRRALTAYADAVRARAEG"
     gene            complement(9355..10113)
                     /gene="fabI"
                     /locus_tag="PKU75_03090"
     CDS             complement(9355..10113)
                     /gene="fabI"
                     /locus_tag="PKU75_03090"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HPF79890.1"
                     /translation="MLLEGKTLLITGVLMDSSIAFHVAKLAQEEGAKVILTSFGRTMK
                     ITQTIAKRLPELPPVVELDVSDQSHLDSLAERLREHTDSLDGVLHSIGFAPQGAFNFL
                     EGTWEDVATAIQISSYSLKSLGAACLPLMSEGGSIVGLTFDAKFAWPVYDWMGVAKAA
                     FESTNRYLARDLGPKGIRCNLVAAGPIRTTAAKSIPGFEQFERVWDERAPLGWDVGDP
                     VPTAKACTVLLSDWFPATTGEIVHVDGGVHAMGQ"
     gene            complement(10163..10873)
                     /gene="fabG"
                     /locus_tag="PKU75_03095"
     CDS             complement(10163..10873)
                     /gene="fabG"
                     /locus_tag="PKU75_03095"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPF79891.1"
                     /translation="MTARNVLVTGGNRGIGKSIAEALAAAGHNVVVTTRSGDAPEGLR
                     AVRGDVTDSASVDAAFAAAEELLGGPVEVVVANAGITKDGLLMRMSDADFDAVVDTNL
                     AGSFRCARRASVGMIKKRFGRIIFISSVVGLYGGPGQTNYSASKSGLVGLARSITREL
                     GGRGITANVVAPGFIETDMTAELPEDRQKEYKAAIPAGRFATPAEVAAAVLFLASDEA
                     AYISGAVIPVDGGLGMGH"
     gene            10970..11296
                     /locus_tag="PKU75_03100"
     CDS             10970..11296
                     /locus_tag="PKU75_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HPF79892.1"
                     /translation="MTRPQPSPQSVTTAPESLREEQGSRIRRYLVMMAIRTICFVAAV
                     PLQGWMRWTAVAAAVVLPYVAVVFANAVKPRAEGTITPVTPVVPMLSHEAETLEGHVV
                     HHPRDP"
     gene            complement(11303..12334)
                     /gene="moaA"
                     /locus_tag="PKU75_03105"
     CDS             complement(11303..12334)
                     /gene="moaA"
                     /locus_tag="PKU75_03105"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPF79893.1"
                     /translation="MTDLPMPTVRSDAPGSGPLVDSFGRIGRDLRVSVTDRCNLRCTY
                     CMPAEGLPWLPKPEMLTDDELIRLIGLFVRLGVRQIRLTGGEPMLRKSLVSVVERIAD
                     LDPRPTIAMTTNGIGLDRLAAPLRAAGLDRVNVSLDTIDPQEFVALTRRDRLGDVEAG
                     LKAAQDAGLTPVKVNAVAMRGINDEDVADLLAWCLDRGYELRFIEQMPLDAGHEWQRG
                     TMVTAGEIRSELEKTYRLTPLDGRGSAPAERYLIDDGPHTVGIIASVSAPFCAACDRV
                     RLTADGQLRNCLFANSESDLRGPLRDGADDADLEAIIRGEMWRKARGHGIGRPDFHQP
                     DRPMSAIGG"
     gene            12450..13787
                     /locus_tag="PKU75_03110"
     CDS             12450..13787
                     /locus_tag="PKU75_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPF79894.1"
                     /translation="MPRELLAGIDAGTTSVKVALLTPEGEQVALGRAPTTWERTTYGE
                     ETDAAALTRTARVALADALAQSPDCRIIGIGIASMAEAGVLVDEAGRVLAPVIAWHDH
                     RDEHELQELIAELGGETYSATTGLPLWTQWSVTKHKWLTRHVPAVAAATKRFSVAEWL
                     AYDLGAGHASEPSLASRTGMLDLRTATWWEPALHWAGAEASLFGPLVPAGTGIGAARG
                     QGALAALDGAVVTVAGHDHQVAAIGMGAHRPGDEFDSAGTAEAILRTSAPDIATDAVR
                     TLTAAGVTVGHHAAPGRWCLLGATQGGLILGKVMAALGVDRDGLPQLDQAAAAADDVP
                     TAVTLGPGSGDVTIDPDAAPGEIWRAATRGITAQAAALSDRLTAASGPRRDLVVAGGW
                     THSAAVRDAKAVAFGPYRLSAVAEAGCRGAALLAGVAAGIYADLESAPSPETA"
     gene            13816..14661
                     /locus_tag="PKU75_03115"
     CDS             13816..14661
                     /locus_tag="PKU75_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPF79895.1"
                     /translation="MPLVPTAQLVAAAREAGHGVAAFNVITLEHAEGIVAGAEQAGVG
                     VILQISENAVKFHGGLLRPIAAGSLEIARAATVDVSVHLDHVEDEGLLHQTADAGLSS
                     VMFDASHLEYEDNVAATRAAADWGHANGLFVEAELGKVGGKDGAHAPGVRTDPGEAAA
                     YVEATGVDALAVAVGSSHAMVERTAALDLDLIREIAAAVPVPLVLHGSSGVADDNLRA
                     AVEAGIVKVNIGTILNVAFTGAVREVLDSDPKIVDPRKYVRPGREQIAAVAARLAGLV
                     SGPRT"
     gene            14658..15611
                     /locus_tag="PKU75_03120"
     CDS             14658..15611
                     /locus_tag="PKU75_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPF79896.1"
                     /translation="MIITLTLNPALDLTYHLADDTLGAVDVHRSTSQTLEASGKGINV
                     SRDLHAAGLATAAVFPSAGATGRHLAELLVAEGVRHAAPASSGETRINSTVLLPHGQT
                     IKINGPGAALTPVDVGAVLDAVDQELTQAGGEEETWLVISGSLAPGAGPEVVTAFVEL
                     AHRHTARCAVDISGPALAAALAAGADLLAPNEIELADLLGVPKPTSVDETAALARRVA
                     REYKAQLLVSMGKAGAIHTDGFSVIHGGGPALVPVNTAGAGDAFLAGWLAAAGTPSER
                     MARAMQWGRSACLCPTTVDPRPGSAGTEGITVSQIETAERP"
     gene            15614..16636
                     /locus_tag="PKU75_03125"
     CDS             15614..16636
                     /locus_tag="PKU75_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPF79897.1"
                     /translation="MNDIRRIRVSAIDTVEVLTEPPAPIGPGLVRVRSVTVGVCGSDT
                     HALHGRHPLVPPPYFPGHEATGVVTELGPDVTGVAVGDRVVVEPTLPCWTCKQCAQGQ
                     ENLCENLQFFGCGFEQGGMAEEFVVRADRLHHIPDDLDDTAASLIEPLSTPVHAGRIA
                     GPLKDKAVVIMGAGAIGLLMLAVAKYDGAKRIVQTDPLPGKRELAGKLGADVVVDAAA
                     PDMVEQVRAALGESADVVFDCVAIQPTITAAIALATKGGTVAIVGVPSADVTIPTITV
                     QDNQIRLQGCATYVPQDYAKSIEILQSGAIDPSMYVTKEFPLEQARDAFDASLSGSEI
                     KVLIRP"
     gene            16633..17424
                     /locus_tag="PKU75_03130"
     CDS             16633..17424
                     /locus_tag="PKU75_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPF79898.1"
                     /translation="MTAAAGVPENQLAAQRHQFILDTLRTRGQVGAKAVADSLGVTHE
                     TIRKDLVLLQERGLLRRVHGGAVPVESLAYEPPVTSRTARGEEKARIAAAAREFVPAT
                     GVVLFDSGTTTTGLAEIMPIAPELMAFTNSLPIAETLLGRVGTLSILGGRVRRETQAS
                     VDNWALRALESLRADVAFLGANAFSLEHGLSTPDEAEAAIKGAMVRAARMRVLLADSS
                     KFGREAIYRYAEIADVDVLVTDSGLDPSDADRLTRECGVEVVRVP"
     gene            17429..18274
                     /locus_tag="PKU75_03135"
     CDS             17429..18274
                     /locus_tag="PKU75_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HPF79899.1"
                     /translation="MLRALLTPRWLGALLLSALFAVAAYHLGHWQYGKHVAKVERNER
                     ISAHFGAAPVPVGSVLTSSPVPIAQEWTHVSATGTYGQAKLVARGRPLDKQVGYEVLA
                     PFRLASGATVLIDRGWIPLGDKGAAEVPAYAAPPPGEVTLTGWVRPGEKARHSSATAG
                     TIASINLPEAAQKFGTPLLGGYVQLEADTPAATAGDALVPQPLGKPDRSLGPHQAYAY
                     QWWTSMPLGFILMFFGLRREAAEGRAERGAGEAPSGAGAGAARTPAPKPPRPKKTRIW
                     DEEDA"
     gene            complement(18297..20174)
                     /locus_tag="PKU75_03140"
     CDS             complement(18297..20174)
                     /locus_tag="PKU75_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79900.1"
                     /translation="MSMPAWRVMHSLTQDSDVADAKLPPGTTRRVLSYAAPFRRAIAG
                     FLVLVVVDSLLIVAAPLLLARLVDNGIMPGDRRVVITLALIVAAIAVVAAVVAVVSRW
                     FSARIGEGLIYHLRSEVFSHVVRQPIAFFTRAQTGALVTRLNSDVIGAQQAFTSVLSS
                     VVSNVVSLALILTAMFTLSWKLTLAALALVPFFLLPARWMGHRLAALAHEQMSLNADL
                     GSRMTERFNVGGALLVKLFGDLEREDREYAERASRVRDIGVKIAMNRTVLFVALTFVA
                     SLATAMVYGFGGVMAIDGTLTIGTLIALTALLARLYGPLTALSNARVDVMTALVSFTR
                     VFEILDLKPSITQSPSAMPLPGGAVDVVFDHVGFAYPGADEVSLRSLEAGASGDRGRG
                     STVLQDISFHIAPGELVALVGPSGGGKTTITNLVARLYDPTSGAVRLQGLDLRDATLA
                     SVTGAVGVVTQESHLFHDTIRANLLYARADATEDAMREALRAAQILPLIDALPQGLDT
                     VVGDRGYRLSGGEKQRIAIARLLLKGPGLLVLDEATAHLDSESELAVQSALDSALAGR
                     TALVIAHRLSTIRRADQILVVDKGRIVERGTHHELLGKGGLYEVLYRTQFQDDSAPVG
                     G"
     gene            complement(20171..20911)
                     /locus_tag="PKU75_03145"
     CDS             complement(20171..20911)
                     /locus_tag="PKU75_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HPF79901.1"
                     /translation="MTTGERRRPDVVLLAHGSPDPRHTRDIAALAHRVAVAAPHVVVH
                     TAYLDHHPPRIADVADAVGDTGSGPIGLVPLLLTQAFHARVDIPAAARELGLRTGRSV
                     VVSAALGPHPLLGSAVGELLDQRGENRAVVLLGGSSRTAAVDDLVAQLRSTLPPVRTY
                     AFATLDDHLPIDAALTALGRAEGVVAVTGILADGVLRDRMVRACAQRGVGVAAGVLAD
                     TDAVTRLVLQRMAGLRESGWDAGGSPQL"
     gene            21014..21361
                     /locus_tag="PKU75_03150"
     CDS             21014..21361
                     /locus_tag="PKU75_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPF79902.1"
                     /translation="MLIRIDETSSTPIYAQIAAAIRREIGAQKLGPGDRLPTARDLAS
                     GLGVNVHTVLRAYQDLRDEGLVDLRRRRGAIVVGDARRAAHLDALKAYVDAASRAGMS
                     AEEAAELVKGRMK"
     gene            21391..22365
                     /locus_tag="PKU75_03155"
     CDS             21391..22365
                     /locus_tag="PKU75_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HPF79903.1"
                     /translation="MTRAWLIVVLVPVLVAATTALVLRSWRGQLPDPVATHWGSGSTP
                     DGFTSLADLPWLGLLALIALPAGCLPLALGGRQGFNRRFAAGFAAGMAVFLGLVVLLS
                     VNAQRGLTDAASAAGDIDGSVALSVLAAVAAGALAAWLVPGSTADAAAALEPIPPDAP
                     RVALAPGERAAWSKRTRSSRIAYALLGGVLALFIAMAIVSGLWLTFAAMGILLAVALA
                     TTLVFRVSVGEDGLVVRGLFGVPRFQTPLSDVAVAQTVSVDPMGEFGGWGFRIGLDGR
                     SGVVVRKGEAIEVTRGDGQRFVVTVDDAARGAALLNTLADRHRTAASR"
     gene            complement(22387..23706)
                     /gene="cobA"
                     /locus_tag="PKU75_03160"
     CDS             complement(22387..23706)
                     /gene="cobA"
                     /locus_tag="PKU75_03160"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPF79904.1"
                     /translation="MSTPAYPITLDVRGRRVLVVGAGPVAERRVRALLASGARVDVIA
                     PRATEGVRHLAEDDAVTWHQRAFDPADLTAPARAWLVHTATGRDEVDQHVCESAAAEA
                     IWSVRAGDSTRSAAWMPAVGAGRGDADGIQVAVTGGGDPRRSTALRDAILIAMETGEL
                     PVARHRHPAQAPVEMSIGPRRALDSAPSTEQSAGRVVLVGGGPGADDLITVRGRRLLT
                     QADVVVTDRLGATGLLDQLPQRTKIIDVGKTPGHHPVPQWEINEILVREAKEGQLVVR
                     LKGGDPFVLGRGGEEMLHCAAHGIPVDVVPGVTSAISVPAAAGIPVTQRGITASFVMA
                     SAHEGPEHVLAAARSAAPDATLVLLMGVSHLDETARGLMAAGRSPQTPVAIIESGWTE
                     RQRTTTTTLESAAADARAAGVRPPAVVVIGEVVGVREELLALEALPG"
     gene            complement(23703..24425)
                     /locus_tag="PKU75_03165"
     CDS             complement(23703..24425)
                     /locus_tag="PKU75_03165"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HPF79905.1"
                     /translation="MSAIPAESLTAEIEAFLVRESGATTYEEKVQLGHDVLAWAHETY
                     GAGLTIASSMGDEILLHLAGTTAPGFDVFFLDTGYHFAETLGTRDAYQVMLPLQIRTV
                     LPRETVASQDIRFGPRLHARNPDLCCRMRKVAPLNAALEGYAAWVTGVRRADAATRTD
                     SQLIEWDAKRSMIKINPVVGWSHDEAERYAAEHGVFMNPLRELGFASIGCEPCTRPVG
                     TGEDPRAGRWANSDKVECGLHI"
     gene            complement(24422..26092)
                     /locus_tag="PKU75_03170"
     CDS             complement(24422..26092)
                     /locus_tag="PKU75_03170"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HPF79906.1"
                     /translation="MTDTVKRPTRTTATGAWADGDRTPLNGNERFKQEDDALNVRRRI
                     IDVYAKEGFAAIPGDDLRGRMRWMGLYTQRKEGIDGGQTATLAPEELDAEYFMMRVRS
                     DGGILSTEQLRTIGQISTDFARDTADISDRQNIQLHWIRIEDVPTIWERLEAVGLHTL
                     EACGDAPRVLLGSPVAGIDAEEIVDGTPAIEEIRRRFIGSPEFSNLPRKFKTAISGSP
                     KLDVVHEINDVSFVGVVHPDLGPGFDVWVGGGLSTNPIFAQRLGAFVTLEQVPDVWAG
                     VIGIFRDYGYRRLRNRARLKFLMADLGPVRFREILETEYLGHALPDGPEPVLPEGVRR
                     DHVGIHAQRDGKVWVGAAPIAGRVSGTVLRGVADLAERHGSHRVRLTPHQKLLVLDID
                     PADADAVAAALLAYDLDARPSEFRRNVLACTGIEYCKLALTETKERARTVVVQLERRR
                     PDFDVPLGIHVNGCPNACARTQVGDIGLKGMVHRTDDGDLEEVFQVHLGGGLGATPSV
                     ARKTRALKVAADDLPDYIERVTGHYLEQRATGESFAAWAHRAEEDDLR"
     gene            26176..26337
                     /locus_tag="PKU75_03175"
                     /pseudo
     CDS             26176..26337
                     /locus_tag="PKU75_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26360..26617
                     /locus_tag="PKU75_03180"
     CDS             26360..26617
                     /locus_tag="PKU75_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79907.1"
                     /translation="MLDLAERLGLDLPHAGDEDAPALREALPPFVGVSNPLDMTAQGL
                     VDPDRYYRTLAAAFADDRFGSVLLGVIQTDPDPAASPGHVE"
     gene            27038..27976
                     /gene="ligD"
                     /locus_tag="PKU75_03185"
     CDS             27038..27976
                     /gene="ligD"
                     /locus_tag="PKU75_03185"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HPF79908.1"
                     /translation="MLDVDGRSVRLSSPNRVYFPEHGWTKEDVARYYLAVGPGILRAL
                     RDRPTMLHRFPDGLDGQKVYQKRLPSGAPDWVPTVRLDFPRYNLHADELAPAHLADVI
                     WACQMSTVEFHPWNVRAADVNRPDEWRIDLDPMPEAGFDAVREVAAVAHEVLDELGIT
                     GYPKTSGGSGLHVYVRIRAEWEFADVRRAAWGFASEVAARMPDQATVTWWRKDRDPKH
                     VFIDYNQNARDHTLAAAYSLRGLPSGTVSSPIEWDELADVDPRDLTLATVPRRFAEIG
                     DPHEKIDDTAYGLETLLEWADRSEASAGPGLGEPES"
     gene            complement(27973..28416)
                     /locus_tag="PKU75_03190"
     CDS             complement(27973..28416)
                     /locus_tag="PKU75_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPF79909.1"
                     /translation="MSDHPRGIIDISVVLSLADLPTQALPAEPVITAITLAELSVGPL
                     VARTAAERAARQAHLQQAEADFEPLPFDAASARAFGRVAASLREAGRKPAARAYDALI
                     AATALANDLPVYTANPRDFAGIDGLTVRDVHGIPGDASPVPGHRA"
     gene            complement(28413..28649)
                     /locus_tag="PKU75_03195"
     CDS             complement(28413..28649)
                     /locus_tag="PKU75_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPF79910.1"
                     /translation="MNTVSIRELRNSGGEVIARVAGGETLLVTRDGEPVARVTPLPRR
                     PASAEVLQERRRCLPRVDADALQADIDDVLDPAL"
BASE COUNT         4412 a        10110 c         9918 g         4273 t
ORIGIN      
        1 cacctacgac ccgacccggg cctccctcgt cctcctcctc gaccgcccct gaccccgccc
       61 cccaaggaac ttctgctagc agaggttagt gaaatcagca gacctcccta ggtgtgctga
      121 tttgactttc ctctgctagc agaggttcct cgggtccggg gtcagccgag gatggagcgg
      181 tagacctcga gggtgcggtc gccgatggag gcccaggaga aggactcgac ggcgcgggcg
      241 cggccggcga ggccgaactg gcgggcgcgg tcggggtcgg agacggcctc gttgagggcg
      301 gcggagaggt cggcgacgaa cttgtccggg tcggtcgggg tgccggtgcc gtcctggacc
      361 tgctcgatcg ggacgagcca gccggtctcg ccgtcgacca cgacctcggg gatgccgccg
      421 gtcgccgtag ccacgaccgg cagttcgcac gccatcgcct cgaggttgac gatgccgagc
      481 ggctcataga cagacgggca cgcgaaaacc gttgcggcag agagcatcgc cgtcacctga
      541 gcgcgcggca acatctccgg gacccagatc acgccgtccc cccgctgctc ccgcaggtcc
      601 gccatcagcc cctcgacctc cgccatgatc tcgggcgtgt cgggggcccc ggccagcagc
      661 acgagctgga cgtccggcgg caggttcgcc gccgcccgca gcagataggg cagccccttc
      721 tggcgggtga tacgaccgac gaagatgacc gacttcttgt cggggtcgac gccgtgacgc
      781 cggacggtgt cgagatccgg attgggttgc cacagttgcg aatcgatgcc gttgtggacg
      841 accttgacct tctccgggtc gatcgacggg tagctgcgca ggatgtcctc gcgcatcccc
      901 gcgctcaccg cgatgaccgc agccgccgcc tcatacgccg tcttctccac ccacgacgac
      961 acgcggtagc cgccccccag ctgctcggcc ttccacgggc gcagcggctc gagcgagtgg
     1021 gcggtgatga catgcggcat accgccgagc aatgacgcga cgtgcccgcc gaagttggcg
     1081 taccacgtgt gcgagtgcac caggtcggcg ccttcggtgc cggcgaccat cgacaggtcc
     1141 acccccatcg tctggatggc ggcgttggcg gaggcgagtt cggccaggtc cgggtatgcc
     1201 gtggtgtcgg cctcgtcccg cggctcgccg aagcagcgga cctggaccgt catgtcgccc
     1261 cgggctcgca gggcccggac cagttcggcg acgtgcaccc ccgctccccc gtagatggcc
     1321 ggcgggtact ccttgctcag aatgtcgacg cgcacatctg caacgtagtc ccgtcggcga
     1381 cgttccggct accgaagggc gaacactctc gggcaatgtg agcggttctc ctcgccgcaa
     1441 cggtcgccga accactaggt tcggatacat ggcaccgcgt aaagaagcga aggttctcgt
     1501 catcgtcctc gccggaggcg aaggaaagcg cttgatgccg ctgaccgcgg accgtgccaa
     1561 acccgcggtg ccgttcggcg ggatctaccg gctcatcgac ttcgccttgt ccaatgtcgt
     1621 caattccggc taccggcagg tcgtggtgct gacgcagtac aagtcgcaca gcctcgaccg
     1681 gcacatcacc aagacgtggc ggatgtcgac gatgctcggc ggttatgtcg ccgcgatccc
     1741 ggcgcagcag cggctgggca agagttggta tgtcggcagc gccgacgcga tctatcagtc
     1801 cctcaacacg attcgcgacg agaagcccga cattgtcgcc gtcctcggtg ccgaccacgt
     1861 ctatcggatg gacttctcgc agatggtcga ccagcaccgc gagaccggtg ccggcatcac
     1921 cgtcagcgcg atccgccagc ccatcgagct ggccgaccag ttcggcgtca tcgaggtcga
     1981 cgccaaggac acgaccaaga tcgcccgctt ccgcgagaag cccaccgacg ccgtgggcct
     2041 gcccgacgcg ccgcacgagg tgctcgcctc gatgggcaac tacgtcttcg acgccgacgt
     2101 gctcgtcgac gccgtcaccc gcgacgccga ggacgagaac agcacgcacg acatgggcgg
     2161 cgacatcgtg ccctccttcg tcgaccagga ccgcgcctat gtctacgact tcaagaacaa
     2221 cgccgtcgac ggcgccaccg aacgcgaccg cggctactgg cgtgatgtcg ggacgctcga
     2281 ctcctacttc gacgcgaaca tggacctggt caacatcaac ccggtcttca acctctacaa
     2341 ctacgactgg ccgttgctga cctcctatgg cgcgctgccg ccggccaagt tcgtccacgg
     2401 ccaccacaac cgcttcggcg aagcgctcaa ctccatcgtc tcccccggct cgatcatctc
     2461 cggcgcccgc gtcaccggct ccgtcgtcgg accgaaggtg cgtgtccaca gctattcgga
     2521 ggtggccgat tcggtgatca tggacaatgt gacgatcaac cgcagttgcc agatccggcg
     2581 caccatcatc gacaaggacg tccagatccc cgagggcacg acgatcggct tcgacctcga
     2641 gcacgacaag gcacgtggct tcaccgtgac cgagagtggc ttggtcgtca tcggcaaggg
     2701 gcagactgtc accccgtgac ggatcctttt gccgcgctgg ccggttatcc ccgcctgctc
     2761 atcgtcagtg acgtcgactc gaccttcatc ggtcaggagg tcatcgagct ggtcgcccgg
     2821 cgggccggga ccgaggagga agtggccgcc gtgacggaag cggccatgcg gggtgaattg
     2881 gacttcgccg cctccttgcg ggcccgcgtc gccacgctcg ccgggctgcc ggcgcgcgtc
     2941 ctcgacgagg tgcgggccga tgtcaccctg accccggggg tgcacgagtt gctggcgtgg
     3001 gcggccgagg agggccatgc ggtggccctc gtctcgggcg ggttccacga gatcgtcgac
     3061 ccgatcgcgc acagcctggg gatccggtat gtccgcgcca atcgcctcgc cgtcgccgac
     3121 ggacggttca ccggcgaggt gagtggtccg atcatcgatg ccgccggcaa ggcggtcgcg
     3181 ctgcggcagt tcgcggctcg cgaggacatc ccgctcgggg cgacgatcgc catcggcgac
     3241 ggcgccaacg acctggacat gatggcgctc gcgggtctca gcgtcgcgtt cgccgcgaag
     3301 cccgtcgtcg cgcagagcgc ggacatcgcg ttgccgggcc cgcggatcga cgacctggtg
     3361 ccgctgctgc ggctggcgcg cggttaggcc gcacgcgcca cacccacggt ctggcggtat
     3421 gccgcgagcc ggcctggatg tcgagccctc accatcgcct gcgccgacta gggtgggcca
     3481 atgcccgatc gtgccgctcc cgcgaccaag ctgctgatca tcggtggcgc cgaggatcga
     3541 gtgggcaagg ccaagatcct caagcgtttc gtcaagctcg ccggcggcaa gtccgcgcgg
     3601 atcgtcatca tcccgacggc gtcgtccttc caggacgagg tggtcacggc gtacaccgag
     3661 gtcttcaccc ggcacggcgc cgagtcctgc gccgtggtca atccgcgcac ccgcatcgag
     3721 gcggacgacg cggccctcgc tcgggtgatc gacgaggcga cgggtgtctt catgagtggc
     3781 ggcaaccagc tcaagctcgc ccaggtcttc gccggcacgg cggccggcga cgccatcctg
     3841 cgcgcgcatc agcgcggcgc cgtcgtgggc ggcacgtcag ccggcgcgtc gatccagagc
     3901 gagttcatga tctccctcgg cgacgagggc atcacgccac ggcaacgctc aacccaactc
     3961 acccatggcc tcggcctgct gtccggggtg atcatcgacc agcatttcgc gcaacggcag
     4021 cggtatggcc ggttgatgtc gatcgtggcc agctcacctg acctgatcgg gatcgggatc
     4081 gacgaggaca ccgcgatcga ggtgaccgat tcgcgcgagt tcaccgtgct gggccgtggc
     4141 ggcgcctacg tcctggattg ccgccgggcc ctgaccgacg cacccgaggc ccgcaccggg
     4201 gcaccgctgc tggtttccgg cgcggtcgtg catgccctcc ccgcaggctc gaccttcgac
     4261 atcgtcaagc tgatgctggt cgacttcgtc gaacaacacc ccgacctcgg cgtcagcgtg
     4321 accactgccg cctcgtcccg cccctgaccc cccacccccc aaggagtctg ccgtgagcat
     4381 cgatcgtccc gcccccgccg gacccccggc tcccgacctg cggatcatcg aagagcgggt
     4441 ctaccgcggc ggcaatatct ggtcctatcg ccccgcggtc catctggtcg tggatctcgg
     4501 tgtcctggag ggctacccga ccaacaccat tcccggattc accgacggac tgctcgagct
     4561 gctgccccgc gtcggcggcc acacctgctc tcgcgggcac gagggcggct tcggtgagcg
     4621 gctgcgcgag ggcacctggg tggggcacgt cgccgagcac gtcgctctcc agttacaaca
     4681 ggaggccggc cacgacgagc gccgcggcaa gacccggatg gtcaaggacc gtccgggggt
     4741 ctacaacgtg gtctacggct acaccgacga gggcgtgggc ctctcggccg gccggctggc
     4801 ggtgcgcctg atcaatcacc tgatcgagcc tgaggacgag ttcgacttcg ccgaggaact
     4861 cgaactcttc ctgcgccgcg ccgaacgcac cgccttcgga ccctcgacgg cagcgatcct
     4921 cgaggaggcg gcgagccggg acatcccctt catccggctc aactccgcgt ccctggtgca
     4981 actcggtcag ggcaagtacg cccagcgcat ccgggcgacg atgacctcgc acaccggtgc
     5041 cttggcggtc gacatcgcct ccgacaagga catgacgatc aagctcctcg gatccgcggg
     5101 cctccccgtg ccgaagcagg agacctgtcg caccgaggac ggagcggtcg aggccgcccg
     5161 ccgcgtcggc tatccggtgg tcgtcaagcc cttggacggc aaccacgggc gaggggtcgg
     5221 gctgaacctg gacaaccccg acgacgtccg cgccgccttc gccgccgcca aggagcagtc
     5281 ccgcaacggc tgggtcatcg tcgagtcgtt catcaccggc aaggactacc ggtgcctgat
     5341 cgtcggcggc cggatgcggg ccatcgccga acgcgtgccc gcccatgtca tcggcgacgg
     5401 caccaacacc atcgagcaac tggtcgccat caccaacgcc gacccgcgcc gcggcgtcgg
     5461 ccacgaaaag gtgttgaccc gcatcgccgt tgacgacgca gcgatcgagc gggtcaagga
     5521 tcagggctac gcgatggacg acgtgccacc cgagggcacg atggtcaaac tggccctcac
     5581 gggcaatatg tcgaccggcg ggatctccat cgaccgcacc ttcgacgccc accccgacaa
     5641 cgtcgagatc gccgaggaag cagcccgggt gatcggcctg gacgtcgccg gcatcgactt
     5701 catctgcccc gacatcaccg ctccggtgcg cgagaccggg ggcgcgatct gcgaggtcaa
     5761 tgccgcgccc ggcttccgga tgcacaccca ccccacggtg ggcgaaccgc agttcatcgc
     5821 caagcccgtc gtcgacctgc tcttcccgcc gggggcgccg tcacgcgtgc cgatcgtcgc
     5881 cgtcaccggc accaacggca agacgacgac ctcgcggatg atcgcccaca tcttcaagga
     5941 aacgggcaag aaggtcggca tgacctccac cgacggcatc gtcatcgacg agcgcctcgt
     6001 catcaagtcg gacgcctccg ggccccgctc ggcccgcatg gtcctccaga acccgcgtgt
     6061 cgaggtcgcc gtgatggagg tcgcccgcgg cggcatcctg cgcgaggggc tcggctacga
     6121 tcgaaacgac atcgcggtcg tcaccaatat cgctcccgat catctcggga tgaagggcat
     6181 cgacacactc gaacaactcg cggacgtcaa ggccgtgatc gtcgaggcgg tcccccgcga
     6241 cggcttcgcc atcctcaacg ccgacgaccc gcacgtgcgc cggatgcgca accgctgctc
     6301 gggaaacatc gtctggtact ccctcaagcc cgaggactcc aaagagcggg cgttcatcga
     6361 tgcccggctg cgccgcggtg gccgggccgt cgtcctcgat cgcaccgaac gcggcgacat
     6421 gatcgtcatc aaacatggtt ctcgctcaat gcaattggcg tggacccacc tgctgccggc
     6481 aaccttcggg ggcacggcga tgttcaatgt cgccaacgcg atggccgctg ccggggcggc
     6541 cttcgccgcg ggcatcggac tgcacgagat ccggcagggg ctgcgcacct tcaccacgag
     6601 ctactacctc tcgccgggcc ggatgaacct catcaacgtg cgcggcatgg aggtcatcgt
     6661 cgactactgc cacaacgctc cgggcatgga ggtgctgggc cagttcgtcg atcgttatgc
     6721 cgaccgcaag gccgcgacct ccgagtgggg caagatcagc cggatcggca tgatcgccac
     6781 cgcgggcgac cgccgcgacg aggacctgcg cgagctcggc agggtcgcgg cacggtactt
     6841 cgacgtgctc gtcgtccgtg aggaccagcg gcttcgtggc cgtcagccgg gtgaggccgc
     6901 cgaactcatc cgcgagggca tccgcgaagc cgacgctgcc ggcggcgtgc gcgtccgcgc
     6961 ggccgagacc gtgctcgacg aggtcgacgc agtgcggcat tgcgtcgccc gtgccaaccc
     7021 gggcgacatc gtcgtgttgt gcgtcgacaa ccacgccaag gtggtcgcgg agctggagac
     7081 gatcacccat tccgcccagg ccggttcgag cggagacgag ccgggcgacc cggacttcga
     7141 cgcgcgcacc tacccggcgg gctagccctc ggcgcgtgcg cgcaccgcgt ccgcgtatgc
     7201 cgtcagtgcg cgccggtagg cgaagctgtc gtcgtcggtg gcccgggcaa gcagtgcgtc
     7261 gatcaggtcg gcgacgcccg tctccgcctg cccggcgtcg aggcaggcca gcgcccggaa
     7321 tgcgatggct gccgcactct cgggtcgttc cgcgacgagg gcgtccaaca gggtgacggc
     7381 ttcccccggc cggccgagat tgcgcagact cgacgcgagc tggatgcggg cccggtgccg
     7441 atgtggctcc ggaaggccgc tatcgagcgc ctcctcatag aggggcaccg cgtcggcttc
     7501 ccgccctgcg gcatccagcg tgccggcgta ttcgtaggcg gctcgggcgt cgccgggcaa
     7561 tgcgcgatgc agtgccgctg ctgcagcgat ccgtgccgac gcgtcggtca tgtcccagag
     7621 attctcgaag tcctcctcgc gcccgtgctt gccctgggcg agcttgacct tgcggcggat
     7681 gtcctcgggc gcgtcggccc gtgcaccccg ctcggccagc caggcgacca tctcggcacg
     7741 ctgctcgtga gtcatgacgg cgatcacgtc gccggcccgc gcgtgctccg aaagcgcttg
     7801 cagggcaacg agttcacttg ggtatgccgc gacgtcgccc acccccgagc gcgccagacc
     7861 ggcgccgaag agcacctcga agtcgtgggt gctgcgaccg cgcagatagt ggtccttgtg
     7921 ggcgatgacg acctgatcgg ctcgctggcc ggcgatctgc ccgagcgcct ccatgatctc
     7981 gtcggtgcgg tcccccgccg tccccagggc gagaaagacg cgacctcccg gggcggccag
     8041 ccctcgggcg acgtcgagca gcgccaccag gcccgcctcg ttgtgggcca ggtcgaggat
     8101 gacggaggcc tcgccgtcgg gtgcggggag cgagtagata ttcattcgcc ccggattgag
     8161 ccggtcgtcc gggttgaagc tgcgcaaccc ctcgacaacc gcctccatgg gcagcccgag
     8221 gcccaggccg gcggccgtcg ccgccagcac attggcggtg ttgtgctgcg agaggccggc
     8281 gagtgtcgcc ggaatctcgg tgagtggcag cactttctga gtcacagcac cgggcaggag
     8341 gatggtgatg tcgccatcga tgaccgtcag cccgcgcccc ttgcggtcga tggcttcgcg
     8401 cagcgccggc gtggtcggat cgagcgcaaa cgcccacgga cgacccggag agtgcgcgtg
     8461 catggcccac acccgggggt cctcgccgtt gagcacggtc cagccgtccg ggcgggtgac
     8521 cgtggtgacg atggccttga cctccgcgag ctgatcgagg gtgtcgacgc cctgcatgcc
     8581 caggtggtcg gcggacacat tcgtgacgac actgacgtcg ttgtgggtga cgcccatccc
     8641 cttgagcagc aggcccccgc gcgcggtctc caagacaccg atctccagac ccggagtctc
     8701 gagaaccccc cgcgccccgg ccggacccga atagtccccc ggctccatga cctcgcccat
     8761 aacgacgacg ccgtctgtcg aactccatgc cgtcttcttg cccgcggtca tagccatgtg
     8821 cgcgagcatt cgcgtcgtcg tcgtcttgcc gttggtcccg gtgatcgacg cgaccggcac
     8881 cttcggactc aatgcggtcg cggcgcttcc gggcgcggtc tcgcgcatct gcgcgccgat
     8941 tcccgcgatg agtggctccg catcggcccc cgaaagcagt tgggagagaa ggggagccaa
     9001 cgactcgccg aggatgcgtg cgcgggcgcg gcggcgccat acggctgcca cgacgacatc
     9061 ggcgggaccc gctccggcgc gcactcgcac accgacccgc tgcgacccgg cggcccgggc
     9121 cagcgcccgg accaggtgct tggccacgcg cccgaggaag gcctgccgct gcaccgaccc
     9181 aggcgcaccc acccgggccc gcatccccag accgtcggcg acggcctgca cctgcgcgag
     9241 cggcgcgtcg agatagccgg gcaaggacag catgaccttc accgccgggc gggtgaaata
     9301 gaggttcgga ccctccagga gacggacctc ggtggcgacg accggctcgg acacctactg
     9361 tcccatcgcg tgcacgccgc cgtcgacgtg cacgatctca cccgtcgtcg ccgggaacca
     9421 gtcgctgagc aagacggtgc aggccttcgc ggtcggcacc gggtcgccca catcccagcc
     9481 caggggcgcc cgctcgtccc agacccgctc gaactgctcg aagccgggga tcgacttcgc
     9541 ggcggtcgtc cggatcgggc cggcggcgac gaggttgcac cgaattccct tggggcccaa
     9601 gtctcttgcc agatagcggt tggtcgactc gaaggcggct ttggccaccc ccatccagtc
     9661 gtagaccggc cacgcgaact tcgcgtcgaa ggtcagaccg acgatggacc cgccctcgct
     9721 catcagcggc agacacgcgg cgccgagcga cttgaggctg taggagctga tctggatcgc
     9781 cgtcgcgacg tcctcccacg tgccctcgag aaagttgaag gcgccctgcg gcgcgaaccc
     9841 gatggagtgc agcaccccgt cgagggagtc ggtgtgctcg cgcagccgct cggccaggga
     9901 gtccagatgc gactgatcac tgacgtcgag ttcgacgacg ggtggcagct ccgggagccg
     9961 cttggcgatc gtctgggtga tcttcatcgt ccgcccgaag gaggtgagga tgaccttggc
    10021 gccctcctcc tgcgccagct tggcgacgtg gaaggcgatg gaggagtcca tgaggacgcc
    10081 ggtgatcagc agggtcttgc cctcgagcag catcagttcg tcctttcggg tatgcggtgt
    10141 ggtcgggtcg gcgagtccgc gctcagtggc ccatgccgag gccgccgtcg acgggaatga
    10201 cggccccgga gatataggcg gcctcgtccg aggcgaggaa gagcaccgcc gcggccacct
    10261 cggccggggt ggcgaaccgg ccggcaggga tcgccgcctt gtactccttc tgccggtcct
    10321 cgggcagttc ggccgtcata tcggtctcga tgaaaccggg agcgacgaca ttggcggtga
    10381 tcccccggcc gccgagctca cgggtgatcg aacgggccag cccgaccaga ccggacttgc
    10441 tggcgctgta gttggtctgg ccgggaccgc catacagccc gaccacgctg gagatgaaga
    10501 tgatccggcc gaaccgcttc ttgatcatcc cgacgctcgc gcgtcgcgcg caccggaacg
    10561 acccggcgag gttggtgtcg acgacggcgt cgaagtcggc gtcactcatc cgcatgagca
    10621 gcccgtcctt ggtgatcccg gcgttggcca ccaccacctc gacgggtccg cccaggagtt
    10681 cttcggcggc cgcgaaggcg gcgtcgaccg acgcggaatc ggtgacatcg ccgcggacgg
    10741 cacgcaggcc ctcgggtgcg tcaccggaac gcgtcgtgac gaccacgttg tgtccggccg
    10801 cggccagggc ctcggcgatc gacttgccga tccctcgatt gccaccggtc accaggacat
    10861 tgcgtgcggt catcgatcgc ctctcacgtt cggggtgctg caggtcacag acgaacctag
    10921 tggacggcgc acctcgatgc acacgccgct cgcggcatac cctgcttatg tgactcggcc
    10981 gcagccctcc ccgcagtcgg tgacgactgc cccggagagc ctgcgcgagg agcagggttc
    11041 gcggatccgc aggtacctcg tcatgatggc gatccgcacg atctgcttcg tcgcggccgt
    11101 gccgctgcag gggtggatgc gctggaccgc cgtcgcagcg gccgtagtgc tgccgtatgt
    11161 cgcggtcgtc ttcgccaacg ccgtcaagcc gcgggcagag ggcaccatca cgccggtcac
    11221 ccctgtcgtg cccatgctct cccacgaagc cgagactctc gaaggccacg tcgttcatca
    11281 cccgagggac ccgtagccgc tctcagcccc cgatggcgct catggggcgg tcgggctggt
    11341 ggaagtccgg tcgtccgatg ccgtggccac gggccttgcg ccacatctcg ccgcggatga
    11401 tggcctccag gtcggcgtcg tccgcgccgt cgcgcaacgg cccgcgcagg tcggattcgc
    11461 tgttggcgaa gaggcagttg cgcaactggc cgtcggccgt caggcgcacc cgatcgcagg
    11521 cggcgcagaa gggcgcgctg acgctcgcga tgatgccgac ggtatgcggt ccgtcgtcga
    11581 tcaggtagcg ctcggcgggt gcgctgccac gaccgtcgag gggggtgagc cggtaggtct
    11641 tctccagttc gctgcggatc tcgccggcgg tgaccatggt gccgcgctgc cattcgtggc
    11701 ccgcgtcgag gggcatctgc tcgatgaagc gcaactcgta gccgcgatcg agacaccacg
    11761 cgagcaggtc ggcgacgtcc tcgtcgttga ttccgcgcat ggcgacggcg ttgaccttga
    11821 ccggcgtcag gccggcgtcc tgagcggcct tgagcccggc ctcgacatcg ccgagccggt
    11881 cccgccgcgt cagcgcgacg aactcctgcg ggtcgatcgt gtcgagggag acgttgaccc
    11941 ggtccagccc ggccgccctg agtggagcgg ccagccggtc caacccgatt ccgttggtgg
    12001 tcatggcgat cgtcggccgc gggtcgaggt cggcgatgcg ctcgaccacg ctcaccagtg
    12061 acttgcgcag catcggctcg ccgccggtga gacggatctg gcggaccccg aggcgcacga
    12121 agagcccgat gagccggatc agctcgtcgt ccgtgagcat ctcgggcttg ggcagccagg
    12181 gaagtccctc ggccggcatg caataggtgc accgcaggtt gcaccggtcg gtcacggaga
    12241 cgcgcaggtc acggccgatc cggccgaagc tgtcgaccag cgggcccgag ccaggcgcgt
    12301 cggagcgcac cgtgggcatc ggcaggtcgg tcatgagaga ccaccctatg cgcgcccccg
    12361 gacaacccgt gattcaagac aaccccttga cacaagcatt cccaaccaac tacaactatt
    12421 cccaacacca cgacgacgga gacgcaggta tgccgcggga actgctggcc gggatcgacg
    12481 ccgggaccac ctcggtcaag gtcgcgctgc tgaccccgga gggggagcag gtcgccctcg
    12541 gccgcgcgcc gaccacgtgg gagcgcacga catacggtga ggagaccgac gccgccgccc
    12601 tgacccgcac cgcccgggtc gccctcgccg acgctctcgc ccagtctccc gattgccgga
    12661 tcatcggcat cggcatcgcg agcatggccg aggcgggggt cctcgtcgac gaggccggcc
    12721 gcgtcctggc accggtgatc gcctggcacg accaccgcga cgagcacgaa ctgcaggagc
    12781 tgatcgccga actcgggggt gagacctact ccgccacgac ggggctgccg ttgtggacgc
    12841 aatggagtgt caccaagcac aagtggctga cccgccacgt gcctgccgtc gccgcggcca
    12901 ccaaacggtt cagcgtggcc gagtggctcg cctacgatct cggcgcgggt cacgccagtg
    12961 agccctcgct cgcctcgcgg actgggatgc tcgacctgcg caccgcgacg tggtgggaac
    13021 cggcgctgca ctgggccggc gccgaggcct cgctgttcgg gccgctcgtc ccggccggca
    13081 ccggcatcgg cgccgcccgc gggcagggtg cgctcgcggc actcgacggc gcggtcgtca
    13141 cggtcgcggg ccacgaccat caggtcgcgg cgatcggcat gggtgcccat cgtcctggtg
    13201 acgaattcga ttctgcggga acggcagagg ccattctgcg cacctccgca ccggacatcg
    13261 ccaccgacgc cgtgcgcacg ctcacggctg ccggcgtgac cgtcggccac cacgcggccc
    13321 cgggccgctg gtgcctgctc ggcgcgaccc agggcggcct catcctcggc aaggtgatgg
    13381 cagcgttggg tgtcgaccgg gacggtttgc cgcaactcga ccaggcggcc gcagcagccg
    13441 acgatgtgcc tacggccgtc accctcggcc ccggctccgg cgatgtcacc atcgaccccg
    13501 acgccgcgcc gggcgagatc tggcgcgcgg cgacgcgcgg catcacggcc caggccgcgg
    13561 ccctgagcga tcggctcacc gccgccagcg gaccccggcg cgacctggtc gtggcgggcg
    13621 gctggaccca cagcgccgcg gtccgggacg caaaggccgt cgccttcggg ccctatcgac
    13681 tctccgccgt cgccgaggcc ggctgccgcg gcgccgccct gctcgccgga gtcgccgccg
    13741 gcatctacgc cgacctcgaa tcggcaccat cccccgaaac cgcttgagtc ccgaacagat
    13801 cgaactggag aaccgatgcc cctcgtcccc acggcccaac tcgtcgccgc cgccagggag
    13861 gccggtcatg gagttgccgc tttcaatgtg atcactctcg agcatgccga aggcatcgtc
    13921 gccggcgccg agcaggcggg ggtcggggtg atcctgcaga tcagcgagaa cgccgtgaag
    13981 ttccacggtg gactgttgcg cccgatcgcc gccggctccc tcgagatcgc ccgtgccgcc
    14041 acggtcgatg tgtctgtgca cctcgaccac gtcgaggacg aaggcctgct gcatcagacc
    14101 gctgacgctg gcctgagttc ggtgatgttc gacgcctcgc acctggaata cgaggacaat
    14161 gtggccgcca cccgcgcggc ggccgactgg ggccacgcca acgggctctt cgtcgaggcc
    14221 gaactcggca aggtgggcgg caaggacggc gcgcacgcac caggtgtgcg caccgacccc
    14281 ggtgaggcgg cggcatacgt cgaagccacc ggtgtggacg ctctcgctgt ggcagtgggc
    14341 agttcgcacg ccatggtcga acgcaccgcg gccctggacc tcgacctgat ccgggagatc
    14401 gcggccgcag tgccggtgcc actggtgctg cacggcagct cgggcgtggc cgacgacaac
    14461 ctgcgcgccg ccgtcgaggc cggcatcgtc aaggtcaata tcggcaccat cctcaatgtt
    14521 gccttcaccg gcgccgtgcg cgaggtcctc gattccgacc ccaagatcgt cgacccgcgc
    14581 aagtatgtcc ggcccggtcg cgagcagatc gccgcagttg ccgcgcgact cgccggcctg
    14641 gtctccgggc cacgcacatg atcatcaccc tcaccctcaa cccggcgctc gacctgacct
    14701 accacctggc cgacgacacg ctcggcgccg tcgacgtgca ccgctccacg agccagacac
    14761 tcgaggccag cggcaagggg atcaacgtct ctcgcgacct gcacgcggcg ggtctcgcga
    14821 cggcggccgt ctttcccagt gccggggcga ccggccggca cttggccgaa ctccttgtcg
    14881 cagaaggcgt ccggcacgcg gctccggcga gctccggcga gacccggatc aactccaccg
    14941 tcctgctccc ccatggccag accatcaaga tcaacggccc gggtgccgct ctcacccccg
    15001 tcgacgtcgg cgccgtgctc gacgccgtgg atcaggaact cacccaggcc ggcggagagg
    15061 aggagacctg gttggtcatc tccggatccc tggcgccggg cgccgggccc gaggtggtga
    15121 cggccttcgt cgagctggcc caccggcata cggcccgctg tgccgtcgac atctccggac
    15181 ccgcccttgc ggccgcattg gctgcgggcg cggatctctt ggcacccaac gagatcgagc
    15241 tggccgacct gctcggtgtg ccgaagccca ccagcgtcga cgagaccgcc gcgctcgccc
    15301 gacgcgtcgc ccgcgagtat aaggcgcagc tgctcgtcag catgggaaag gcgggcgcga
    15361 tccataccga tggcttctcg gtcatccacg gcggcggccc cgcactcgtg cccgtcaaca
    15421 ccgcgggcgc cggcgacgcc ttccttgccg gctggctggc ggcggcgggc acgccgtccg
    15481 aacgcatggc gcgggccatg cagtggggcc gctccgcctg tctgtgcccc accaccgtcg
    15541 accctcggcc gggatccgcc ggcaccgagg gcatcaccgt ctcccagatc gaaaccgctg
    15601 aaaggccttg accatgaacg acatccgccg tatccgtgtt tccgcgatcg ataccgtgga
    15661 ggtgctgacc gagcccccgg cgcccatcgg cccgggtctg gtgcgcgtcc ggtccgtgac
    15721 cgtcggcgtc tgcggttcgg atacgcacgc tctccacggg cgccacccgc tcgtgccgcc
    15781 gccctacttt cccggccacg aagccaccgg cgtggtcacc gaactcggcc cggacgtgac
    15841 cggcgtggcc gtcggcgacc gggtggtggt cgagccgacc ctgccctgtt ggacgtgcaa
    15901 gcagtgcgcg cagggtcagg agaacctgtg cgagaacctc cagttcttcg gctgtgggtt
    15961 cgagcagggc gggatggcgg aggagttcgt cgtgcgggct gatcggctgc accacatacc
    16021 cgacgatctc gacgacaccg ccgcctcact catcgagccg ctgtcgacgc ccgtgcacgc
    16081 gggccggatc gccgggcccc tcaaggacaa ggcggtcgtc atcatgggcg cgggcgccat
    16141 cgggctgctc atgctcgccg tggccaagta tgacggggcc aagcgcatcg tgcagaccga
    16201 tccgctgccg ggcaaacgcg aactcgccgg caagctcggc gcggacgtcg tcgtggacgc
    16261 ggcggccccc gacatggtcg agcaagtcag ggcggcactt ggcgagagcg ccgatgtcgt
    16321 cttcgactgc gtggcgatcc agccgacgat caccgccgcc atcgcactgg ccaccaaggg
    16381 cggcaccgtc gccatcgtcg gcgtgccgtc cgcggacgtg acgattccca cgatcacggt
    16441 gcaggacaac cagatccgcc ttcagggttg cgccacctat gtgccgcagg actacgccaa
    16501 gtccatcgag atcctgcagt ccggcgcgat cgacccaagc atgtacgtga ccaaggagtt
    16561 cccgctggag caggctcgcg acgccttcga cgcctcgctc agcgggtccg agatcaaagt
    16621 gctgatccgg ccgtgacggc cgcggccggc gtcccggaga accaactcgc ggcgcagcgt
    16681 caccagttca ttctcgacac cctgcgcacc cgcggccagg tgggagccaa ggcggtcgcc
    16741 gacagcctcg gcgtgacaca cgagacgatc cgcaaggacc tcgtcctgct gcaggaacgc
    16801 ggactgctgc gccgcgtcca cggcggggcg gtgcccgtcg agtcgctcgc ctacgaaccg
    16861 ccggtgacct cacgaaccgc tcgtggtgag gagaaggcgc ggatcgcggc ggcggccagg
    16921 gagttcgtgc cggccaccgg agttgtgctc ttcgactccg gcaccaccac caccgggctc
    16981 gcggaaatca tgccgatcgc cccggaactg atggccttca cgaactcgct tcccatcgcc
    17041 gagacccttc tcgggcgggt cggcacgctg tccatactcg gcggtcgcgt ccgccgcgag
    17101 acgcaggcca gcgtggacaa ttgggccttg cgcgccttgg aatcgctccg cgccgacgtg
    17161 gccttcctcg gcgccaacgc gttctccctc gagcacgggc tctccacgcc ggacgaggcg
    17221 gaggcggcca tcaagggcgc gatggtgcgg gcggcgcgca tgcgggtgtt gctcgcggac
    17281 agttcgaagt tcgggcggga ggccatctat cgctatgccg agatcgccga tgtcgacgtc
    17341 ctcgtgaccg actcgggtct ggaccccagc gacgccgatc gcttgacccg cgagtgcggc
    17401 gtggaggtcg tgcgggtacc gtgacggggt gttgcgcgcc ctgctgaccc ctcgctggct
    17461 gggcgccctc ctcctgtcgg ccctgttcgc cgtcgccgcc tatcacctcg gccactggca
    17521 atacggcaag cacgtcgcga aggtcgagcg caacgagcgg atcagcgcgc acttcggtgc
    17581 cgcaccggtg ccggtgggct cggtgctcac ctcctccccc gtgcccatcg cgcaggagtg
    17641 gacgcacgtc agcgcgacag gcacctacgg acaagccaag ctggtcgccc ggggccggcc
    17701 gctggacaag caggtggggt atgaggtgct ggcccccttc cgcctggcct ccggggccac
    17761 ggtgctcatc gaccgcggct ggatcccgtt gggcgacaag ggagctgccg aggtccccgc
    17821 gtatgccgcg ccgccgcccg gcgaggtgac gctcaccggc tgggtacgcc cgggcgagaa
    17881 ggcacgccat agctccgcga cggccggcac catcgccagc atcaacctgc cggaggctgc
    17941 gcagaagttc ggcactcccc tcctgggcgg gtatgtccag ctggaggcgg acactcccgc
    18001 tgccacggcg ggcgacgccc tggtgccgca gccgctcggc aagccggacc gcagtctcgg
    18061 cccgcaccag gcctacgcct accagtggtg gacctcgatg ccgctcggct tcatcctgat
    18121 gttcttcggc ctgcgccgcg aggccgccga aggtcgtgcc gagcgtgggg ccggcgaggc
    18181 gccgtccggg gccggggcgg gggcggcccg gaccccggca ccgaagccgc cgcggccgaa
    18241 gaagacccgc atctgggacg aggaggacgc ctgatctccc gttgccaggt aggccctcag
    18301 ccccctactg gcgcggaatc gtcctggaac tgggtgcggt agaggacctc atacagcccg
    18361 cccttgccca gcaactcgtg gtgggtcccc cgctccacga tccgcccctt gtcgacgacg
    18421 aggatctggt cggcccggcg gatggtcgag aggcgatggg cgatcaccaa ggcggtgcgg
    18481 cccgccaggg cgctgtccag cgccgactgc acggccagct cggactcgct gtccaggtgg
    18541 gcggtggctt cgtcgagcac gagcagcccc ggccccttca gcagcagccg ggcgatggcg
    18601 atgcgctgct tctccccgcc ggagagccgg tagccccggt cgccgacaac cgtgtcgagg
    18661 ccctggggca acgcatcgat cagcggcagg atctgggccg cgcgcagggc ctcccgcatc
    18721 gcgtcctcgg tggcgtcggc acgggcatac agcaggttgg cgcggatcgt gtcgtggaag
    18781 aggtggctct cctgggtcac gacgccgacc gcgccggtaa ccgaggcgag cgtggcgtca
    18841 cgcaggtcca gcccctgcag gcggacggcc ccggaggtcg ggtcatacag gcgcgcgacg
    18901 agattggtga tcgtcgtctt gccgccgcca ctcggcccga ctaacgcgac cagttctccg
    18961 ggggcgatgt gaaagctgat gtcctgcaac acggtcgacc ctcgaccccg gtcaccggag
    19021 gccccggcct ccaacgaccg caaggagacc tcgtcggcac cgggataggc gaagccgacg
    19081 tgatcgaaga cgacgtcgac cgcgccgccg ggcaggggca tggctgacgg cgactgcgtg
    19141 atcgacggtt tcaggtcgag gatctcgaag acccgggtga aggagaccag tgcggtcatg
    19201 acatcgacgc gagcattgct cagcgccgtc aacgggccat acagccgcgc gagcagcgcc
    19261 gtcaacgcga tgagcgtgcc gatcgtcagg gtgccgtcga tggccatcac gccaccgaac
    19321 ccatagacca tggccgtggc gagcgaggcc acgaaggtca gcgccacgaa gaggacggtg
    19381 cggttcatcg cgatcttgac cccgatgtca cgcacccggg aggcgcgctc ggcatactcg
    19441 cggtcctcac gctcgagatc cccgaaaagc ttcaccaaca gggccccacc gacgttgaac
    19501 cgctccgtca tccgcgagcc gagatcggcg ttcaggctca tctgttcgtg cgccagggca
    19561 gccaggcgat gacccatcca gcgggccggc agcaggaaga agggcacgag cgcgagcgct
    19621 gccagcgtga gcttccacga cagcgtgaac atggcggtga ggatcagcgc gagcgagacg
    19681 acattgctga ccacgctcga caggacgctg gtgaaggcct gttgcgcgcc gatgacgtcg
    19741 ctgttgagcc gggtgacgag cgcgccggtc tgtgcgcgtg tgaagaacgc aatcggttgc
    19801 cgcacaacgt gactgaatac ttcactgcgc aagtgataga tgaggccctc accgatgcgg
    19861 gcggagaacc agcgcgacac cacggcaaca acggcggcca cgacggcgat cgccgcgacg
    19921 atgagcgcga gcgtgatgac cactcgccgg tcccccggca tgatgccgtt gtcgaccagt
    19981 ctcgccagca gcagcggggc ggcgacgatg agcaacgagt cgacgacgac gagcacgagg
    20041 aagccggcga tggcgcggcg gaagggcgcg gcatacgaca gcacccggcg ggtggtcccg
    20101 ggcgggagct tcgcgtcggc gacgtccgag tcctgagtga ggctgtgcat cactctccag
    20161 gcgggcatgc tcatagttgt ggtgaacctc ctgcgtccca gcctgattcc cgaaggccgg
    20221 ccatccgctg gaggacgaga cgcgtgacgg cgtcggtgtc tgcgagcacg ccggcggcga
    20281 ccccgacgcc ccgctgggcg caggcccgca ccatccggtc ccgcagcacc ccgtcggcga
    20341 ggatgccggt gactgcgacg actccctccg cccgcccgag tgccgtgagg gcggcgtcga
    20401 tcggcagatg atcatcgagg gtggcgaagg cgtaggtccg gaccgggggg agcgtcgacc
    20461 gcagctgcgc gaccaggtcg tccacggcgg cagtgcgcga cgaaccgccg aggagcacca
    20521 cggcccgatt ctcgccgcgc tggtcgagca gttcgccgac cgcggagccg agcagcggat
    20581 gcggcccgag cgccgcgctg accacgacag atcgtccggt gcgcagtccc aactcccgag
    20641 cggcggcggg gatgtcgacc cgggcgtgga aggcctgggt gagcagcagc gggacgaggc
    20701 cgatcggtcc ggacccggtg tcgcccacgg cgtcggcgac gtccgcgatc cgtggtgggt
    20761 ggtgatccag gtatgccgtg tgcaccacga cgtgcggcgc ggcgacggcg acgcggtgag
    20821 ccagggcggc gatgtcgcgg gtatgccgcg gatccggcga cccatgagcg agcaggacga
    20881 catcgggtcg ccgccgctcg ccagtggtca tggccgaacg atagatgccg cggcagcggc
    20941 gcacctgatc ggtgtttgcc gcgccggtcc gctcgttagt ttgttctatc gcaattagag
    21001 atatgctcga ggcatgctga tccggatcga cgagacctcg tcgacgccga tctatgccca
    21061 gatcgccgcc gccatccgcc gcgagatcgg cgcccagaag ctcgggcctg gcgaccggct
    21121 ccccacggct cgtgacctgg cgtccggtct cggcgtgaac gtccataccg tgcttcgggc
    21181 ctatcaggac ctgcgcgacg agggcctggt cgacctgcgc cggcgccggg gcgccatcgt
    21241 cgtcggcgac gcccggcggg cggcgcacct ggatgcactc aaggcctatg tggatgcggc
    21301 gtcgcgggcg gggatgtccg cggaggaggc cgcagagttg gtgaaaggga gaatgaaatg
    21361 acccgcacgt ccgcttccac tcctcggagc atgacgcgcg cctggctcat cgtcgtgctc
    21421 gtgcccgtcc tcgtggcggc caccaccgcc ctcgtcctcc ggagctggcg cggacaactg
    21481 cccgatcccg ttgcgacaca ctggggatcg ggcagcacgc ccgacggctt cacatcattg
    21541 gcggatcttc cgtggctcgg cctgttggct ctcatcgccc tcccggccgg ctgcctgccc
    21601 ctcgccctcg ggggacgcca gggattcaac cgccgcttcg cggcgggttt cgcggcgggt
    21661 atggccgtct tcctcggcct cgtcgtgctg ttgagcgtca atgcccagcg cggcctgaca
    21721 gacgcagcgt cggccgcagg cgacatcgac gggtccgtcg ctctctcggt gctcgccgcg
    21781 gtggccgccg gcgctctcgc agcgtggctg gtgccgggat cgaccgcgga tgccgcggcc
    21841 gcgctcgaac cgattccacc ggacgcgccc cgagtcgcct tggcacccgg cgagcgggcc
    21901 gcctggtcga aacgcacccg gtcctcccgg atcgcctatg cgctgctcgg cggagtcctg
    21961 gcactcttca tcgccatggc gatcgtgagc ggcctgtggc tcacgttcgc cgccatgggg
    22021 atcctgctcg ccgtcgccct ggccacgaca ctggtcttcc gggtgagcgt cggcgaagac
    22081 ggactggttg tgcggggcct gttcggcgtc ccgcgattcc agacgccctt gtcggatgtc
    22141 gctgtggcac aaaccgtctc ggtcgacccc atgggcgagt tcgggggctg gggtttccgc
    22201 atcggtctcg acggacgcag tggcgtcgtc gtccgcaagg gcgaggccat cgaggtcacg
    22261 cgcggtgacg gtcagcgctt cgtcgtcacg gtcgacgacg cggcccgagg cgcggccctg
    22321 ctcaacaccc tggccgatcg gcaccgcacg gccgcgtcgc gctgacgcaa cgtccggcca
    22381 cgcccctcag cccggcaacg cctccagcgc cagcagctcc tcccggaccc ccacgacctc
    22441 accgatgacg acaacggccg gcggccgcac cccagcagcg cgcgcatcgg ccgcggccga
    22501 ctccagcgtc gtcgtcgtgg tgcgctgacg ctcggtccag ccggactcga tgatggcgac
    22561 gggcgtctgc ggggagcggc ccgccgccat gagcccgcga gccgtttcgt cgaggtgcga
    22621 gacccccatg aggaggacca gggtcgcatc cggggcagcc gaacgggcag ctgccagaac
    22681 gtgttccggc ccctcgtgcg ccgaagccat gacgaaggat gcggtgatgc cccgctgcgt
    22741 gacggggatg ccggcggcag cgggcacgga gatcgccgac gtgacaccag gcacgacgtc
    22801 gaccggtatg ccgtgtgccg cgcagtgcaa catctcctcg ccgccgcggc cgaggacgaa
    22861 ggggtctccg cctttgagcc ggacgacgag ctggccctcc ttggcctcgc gcacgaggat
    22921 ctcgttgatc tcccactgcg gcaccgggtg atgcccgggc gtcttgccga catcgatgat
    22981 cttcgtgcgc tgcggcagct ggtcgagcaa gccggtcgcg ccgagccggt cggtcaccac
    23041 gacgtcggcc tgcgtcagca ggcgccggcc acggaccgtg atcaggtcat cggcacccgg
    23101 accgccgccg accaggacga cccggccggc cgattgctct gtcgacgggg cggaatcgag
    23161 agctcgacgg ggtccgatcg acatctcgac gggggcttgg gcggggtggc ggtggcgggc
    23221 gacggggagt tcgccggtct ccatagcgat gaggatggcg tcgcgcaggg cggtggagcg
    23281 gcgggggtcg cccccgccgg tgacggcgac ctggatgccg tcggcgtcac ccctgccggc
    23341 gccgaccgcc ggcatccagg ccgcagatcg cgtggagtct cccgcccgca cactccagat
    23401 ggcttccgcc gcggcggatt cgcagacgtg ctgatcgacc tcgtcgcgac cggtggcggt
    23461 gtgcacgagc cacgcgcgcg ccggtgcggt caggtccgcc gggtcgaagg cccgctggtg
    23521 ccaggtgacg gcgtcgtcct cggcgaggtg ccgcacgccc tcggtcgccc ggggggcgat
    23581 gacgtcgacg cgggcgcccg aggcgagcaa ggcccggacg cggcgctcgg ccaccgggcc
    23641 ggcgccgacg acgagcacgc ggcggccgcg cacgtcgagg gtgatcgggt aggcgggggt
    23701 gctcatatgt gcagcccgca ttcgaccttg tcggagttgg cccagcgccc ggcgcgcggg
    23761 tcctcgccgg tgccgaccgg gcgggtgcag ggctcgcacc cgatggacgc aaagcccagc
    23821 tcccgcaacg ggttcatgaa gacgccgtgc tccgcggcat accgctcggc ctcgtcgtgc
    23881 gaccacccga ccactgggtt gatcttgatc atcgagcgct tggcgtccca ctcgatcagc
    23941 tgcgagtcgg tgcgggtcgc ggcgtcggcg cgacgcacgc cggtgaccca ggcggcatac
    24001 ccctccaggg cggcgttgag cggcgcgacc ttgcgcatcc ggcagcacag gtcggggttg
    24061 cgggcgtgca gccgcgggcc gaagcgaatg tcttggctcg caacggtttc gcgcggcagg
    24121 accgtgcgga tctgcagggg gagcatgacc tgataggcat cgcgggtgcc cagtgtctcg
    24181 gcgaagtgat agccggtgtc gaggaagaag acatcgaagc ccggcgcggt ggtgcctgcc
    24241 aggtggagga ggatctcatc gcccatcgaa ctggcgatgg tcaggccggc gccgtaggtc
    24301 tcgtgtgccc aggccagcac gtcgtgaccg agctgcacct tctcctcgta ggtcgtcgca
    24361 cccgactcgc ggacgaggaa ggcttcgatc tcggccgtca ggctctcggc ggggatagcg
    24421 ctcatcgcag gtcgtcctcc tccgcgcggt gcgcccaggc ggcgaaggac tcccccgtcg
    24481 cgcgctgctc cagatagtgg ccggtgacgc gttcgatgta gtcgggcaga tcgtcggcag
    24541 cgaccttgag cgcccgggtc ttgcgggcca ccgacggggt ggcgcccagc ccgccgccga
    24601 ggtggacctg gaagacctct tccagatccc cgtcgtcggt gcgatgcacc atgcccttca
    24661 ggccgatgtc tccgacctgg gtccgggcgc acgcgttggg gcagccgttg acgtggatcc
    24721 cgagggggac gtcgaagtcg gggcggcggc gctcgagctg aacgacgacc gtccgcgcgc
    24781 gctccttggt ctcggtcagc gccagcttgc aatactcgat cccggtgcag gccaacacat
    24841 tccggcgaaa ctccgacggg cgggcatcga ggtcgtaggc cagcagggca gctgcgacgg
    24901 cgtccgcgtc cgcggggtcg atgtcgagga cgagcagttt ttgatgcggg gtcaggcgca
    24961 cgcggtgcga cccatggcgc tcggccaggt cggcgacccc acgcaacacc gtgccgctca
    25021 cgcggccggc gatcggagcg gcaccgaccc acaccttgcc gtcccgctgg gcatggatgc
    25081 cgacatggtc gcggcgcaca ccctcgggca gcacgggctc cgggccgtcg ggcagggcgt
    25141 gcccgaggta ttcggtctcg aggatctcgc ggaagcgcac cggacccagg tcggccatga
    25201 ggaacttcag ccgggcccgg ttgcgcagtc gccgatagcc gtagtcgcgg aagataccga
    25261 tgacgccggc ccagacatcg ggcacctgct cgagagtgac gaacgcgccg agtcgctggg
    25321 cgaagatcgg gttggtggac agcccgccgc ccacccagac atcgaagccc ggccccaggt
    25381 cggggtggac gacgccgacg aaggagacgt cgttgatctc gtggacgacg tcgagcttgg
    25441 gcgagccgga gatggcggtc ttgaacttgc gcggcaggtt cgagaactcg gggctgccga
    25501 tgaagcggcg ccggatctcc tcgatcgcgg gggtgccgtc gacgatctcc tcggcgtcga
    25561 tgcccgcaac gggagagccg aggagcacgc ggggggcgtc gccgcaggcc tcgagcgtat
    25621 ggagtccgac agcctcgagc cgctcccaga tcgtcggcac atcctcgatc cggatccagt
    25681 gcaactggat gttctgccgg tcggagatgt cggcggtgtc ccgggcgaag tcggtgctga
    25741 tctgcccgat cgtgcgcagc tgctcggtgg acaggatccc gccgtcgctg cgcacccgca
    25801 tcatgaagta ctcggcgtcc agttcctcgg gggcgagcgt cgccgtctgg ccgccgtcga
    25861 tgccctcttt gcgctgggtg tacaggccca tccaccgcat acggccgcgc aggtcgtcgc
    25921 cggggatggc ggcgaagccc tctttggcat agacgtcgat gatgcgccgg cgcacgttga
    25981 gcgcgtcgtc ctcctgcttg aagcgctcgt tgccgttcag cggggtgcgg tccccgtcgg
    26041 cccaggcacc ggtcgcggtg gtccgggtcg ggcgtttgac cgtgtctgtc acgccgcaaa
    26101 tctacaacac caaattatga cgtacataac gcgttcttat gctgaacgcc ttacgttctc
    26161 tgcgcccggc aggcggccac acacacgggc gcgatggccg gcgattacga cgtgatgcgc
    26221 gtgcaggtgg agcgggcggg tgtgttgctc atcggctccc tcgaggagct gggtgatgtg
    26281 gcggagatcg ccgtccgctc accgcgtccg ggctccggcg gcccccatcg tcggtgagtc
    26341 cggggccttc aaggccttga tgctcgacct ggccgaacgc cttggcctgg atctgccgca
    26401 tgccggcgac gaggatgccc ccgcgttgcg cgaggcgttg ccgcccttcg tcggcgtgag
    26461 caatccgctg gacatgacgg cgcaggggct ggtcgatccg gaccgctact accgcacgct
    26521 cgcggccgcc tttgccgacg accgcttcgg cagcgtgctc ctcggcgtca tccagaccga
    26581 cccggacccc gccgcgagcc cgggccacgt cgagtgagcg cgacacgccc acgatccggc
    26641 acgaacctgg gcgtgtcgcg ctcactcgag gattctgtcc ccggtcgtgg gccaccatgg
    26701 gcgggtgagc gaggacaagc actggcccga gagtctcagc gcgtccgcgc gacgagccct
    26761 cgaggcgcgc ggctggggag gacttgccga cctggcgggc gcgacgatgg tcgaggtcgc
    26821 ctccgtcaag ggcgtcgggc ccacggtgat catggtgttg gcggcagcgc tggacgaggc
    26881 gggcacgccg ttcgccgatg cgagcaaggg gccacgtgcg tggaaacagg ctgcgcaaga
    26941 ggcgggttgg ggtcggaggg ggtcggagaa gtgggacggc gccgcagcgc cggcgaagcg
    27001 ttcgacggcc aagaaggagc ccgccggcga gccgatcatg ctcgacgtcg acgggcgctc
    27061 agtgcggctc tccagcccga accgcgtcta cttcccggag cacgggtgga ccaaggaaga
    27121 cgtcgcgcgc tactacctcg cggtgggccc cggcatactg cgcgctcttc gcgaccggcc
    27181 gacgatgctg caccgcttcc ccgacgggct cgacgggcag aaggtctatc agaagcgtct
    27241 gccctccggc gctcccgact gggtgccgac ggtgcggctg gacttcccgc gctacaacct
    27301 gcacgccgac gaactcgccc cggcgcacct ggcagatgtc atctgggcgt gccagatgtc
    27361 cacggtcgag ttccacccgt ggaatgtgcg cgccgccgat gtgaaccgcc ccgacgaatg
    27421 gcgcatcgac ctcgacccga tgcccgaggc cggtttcgac gcggtgcggg aggtagccgc
    27481 cgtcgcccac gaggtgctgg acgagttggg gatcaccggc tatccgaaga cctcgggcgg
    27541 atcggggtta cacgtctatg tgcggatccg tgcggagtgg gagttcgccg acgtccggcg
    27601 ggcggcgtgg gggttcgcct ccgaggtcgc cgcgcgcatg ccggatcagg cgaccgtgac
    27661 ttggtggcgc aaggaccgcg acccgaagca cgtcttcatc gactacaacc agaacgcgcg
    27721 cgaccacacc ctcgccgcgg catacagcct gcggggcctg ccctccggca ccgtctcgtc
    27781 gcccatcgag tgggacgaac tcgccgacgt cgacccccgc gacctgaccc tggccaccgt
    27841 gccgcgccgg ttcgccgaga taggcgaccc gcacgagaag atcgacgaca cggcatacgg
    27901 gctggaaacc ctgctcgagt gggccgaccg ctcggaagcg tcagccggcc caggcctggg
    27961 agagccggaa agctaggcgc gatgccctgg cacagggctg gcatcgcccg gtatgccgtg
    28021 cacgtcgcgc acggtgagcc catcgatgcc tgcgaaatca cgcgggttgg cggtatagac
    28081 cggcaggtcg ttcgccaggg ccgtcgcggc gatcagggcg tcgtatgccc gggccgcagg
    28141 ctttcgccca gcctcgcgta gcgacgcggc gacccggccg aaggctcgcg cggaggcggc
    28201 gtcgaagggc agcggctcga agtccgcctc ggcctgctgc agatgcgcct ggcgagcggc
    28261 ccgctcggcc gcggtcctgg cgacgagggg tccgacgctc aattccgcaa gagtgatcgc
    28321 cgtgatgacg ggttccgccg gaagtgcctg tgtcggcagg tcggcgagac tgagcacgac
    28381 agagatgtcg atgatgccgc gcgggtgatc actcacagcg ccggatccag cacgtcgtcg
    28441 atatcggcct gcaatgcatc agcatcgacc ctcggcaagc agcggcggcg ctcctgcaag
    28501 acctccgccg acgcggggcg acgcggcagc ggagtgacac gggcgaccgg ctcaccgtct
    28561 cgggtgacga gcaaggtctc cccgccggca acgcgggcaa tgacctctcc cccgctgttg
    28621 cgcagctcac gaatgctcac cgtgttcacc cagcgaatgt atcccggtga gacgtggctt
    28681 caggcgagcg cgatgaggtc gaggtagtcg ggt
//
LOCUS       DAOPJG010000017        26455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_1953_length_26455_cov_7.724583, whole genome shotgun sequence.
ACCESSION   DAOPJG010000017 DAOPJG010000000
VERSION     DAOPJG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 26455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26455
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1953_length_26455_cov_7.724583"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(836..1564)
                     /locus_tag="PKU75_03200"
     CDS             complement(836..1564)
                     /locus_tag="PKU75_03200"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF79911.1"
                     /translation="MRLILIRHGQSGNNLIWEQTGGSVGRHPDTPLTDRGHEQARKLA
                     AAVADGVLPWRIGALYCSLMTRAVQTAAPLADALDLPLRHLDIFESDGPYDEDPATGA
                     RTPHAGAGRTALLGLSRRLELADDVDESGWWRGDVENADGRLARATRVLEEVRQRHTS
                     LWPTDSGTAGGSAARDAVALVTHGHFTQYLLRAFLSIDTIGGWIDIDNTSVTLLEEQA
                     AYDNTPVAVRINWTPHLTEAERTT"
     gene            complement(1602..1964)
                     /locus_tag="PKU75_03205"
     CDS             complement(1602..1964)
                     /locus_tag="PKU75_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79912.1"
                     /translation="MTSPTAPVRARLRAALGVAMKARDRERVSALRSALARIENAEAV
                     PLTEDFRAGAVENAALGVGAADAARRDLSEREQRSLVQAEADEADAAAAQVESLDPDR
                     ADRLRRTAADLRALVCAG"
     regulatory      2156..2286
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2406..3170
                     /locus_tag="PKU75_03210"
     CDS             2406..3170
                     /locus_tag="PKU75_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HPF79913.1"
                     /translation="MSSLVMAAAADQNIFQRLIEAAIPIGKYQLHYLELIGVLIGVVS
                     AYFGMKRRVWAWPVGITANIMLFFVYIGALVAADERIPLFGQAGRQIFFIATSIYGWW
                     RWNAIRKARGAASDAPAITPRWATTRERIALVVGWLLATVVAHQVFVWLWNLSPNPYW
                     TPQWWYYWCDAWIFVGSFIATFAMARGWNEFWLAWIGVDLVGVPLGFATNYVPTAVMY
                     SFYGLFVIYGFTQWVKASRQEAPTAQDREAVPTAAG"
     gene            complement(3210..3641)
                     /locus_tag="PKU75_03215"
     CDS             complement(3210..3641)
                     /locus_tag="PKU75_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPF79914.1"
                     /translation="MRASAHPEPAPPGGFAGATPDRRRGCRTACASARSEVTPGMAAF
                     DEETFGPVAAITVARDDDHAVELANSTDYGLGLSVWSGATEHAVAVARRITSGAAFVN
                     AIVASDPRVPFGGTKRSGYGRELADAGLLAFTNTRTFWVAS"
     gene            3548..4204
                     /locus_tag="PKU75_03220"
     CDS             3548..4204
                     /locus_tag="PKU75_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPF79915.1"
                     /translation="MRRPSGSHAVYQELRRRILQEELAPGERLHESALAADLGVSRTP
                     YREAVRLLMAEGLLDQLPTGGVVVRGVSARDIEELYAVRAALEGLMAACAATRVDAAA
                     VTRLESLVERNARLVDLPQEAAEAGHGLHLAIAEIAGNDWATKLHSQVDAQMSRYRHY
                     TNETQKRREAALDEHRRIVEALRTGNAATARSAAESHVLAARDTALASIAGRLRAQRL
                     "
     gene            4254..4997
                     /locus_tag="PKU75_03225"
     CDS             4254..4997
                     /locus_tag="PKU75_03225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF79916.1"
                     /translation="MAVELILVRHGQSEGNVAAEAADRDRLEQIAAPARDPDVMLSET
                     GRTQAAAVGAYLSALPADQRPGIVWTSPYQRARETAEIALHAYDAELDFRVDERLRDR
                     DMGITDMLTGAGIRAKYPEEAKRRAWIGKFYYRPPGGESWADVAFRVRSVLTDLANSE
                     KHDRVLVTAHDVVILLFCYVAEGMDEKAVLERATTNGLRNAAICRIRRDEDSATGWTV
                     TAYNEDSHLRDAGVAVTSQPGASDEVGAT"
     gene            4994..5920
                     /locus_tag="PKU75_03230"
     CDS             4994..5920
                     /locus_tag="PKU75_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPF79917.1"
                     /translation="MSGSPRGIPEHASAGTADAPRLVTPALLREWPLPEPTGTKLGRG
                     RVVVVGGARKTPGGAMLGGLAALRMGAGRLSLAVAESVAVQVAVAIPESGVHGLRESA
                     SGSVTGAGAAGILAPELEHADGLLVGPGLDDPAGTIRLLEEVVPVYAADRAIVLDAFG
                     ATVLPDVADGCRKALAGRTLLTPNTNELARLARRTELDEDDVPAAALEVGEEFGAIVS
                     CGGWITADGGVWRITTGDTGLGTSGSGDVLAGAVVGLVARGVAPLPAMLWATYIHASA
                     GDRLATEFGRVGFLAGELLPQLPRALRLLRGD"
     gene            complement(5947..6183)
                     /locus_tag="PKU75_03235"
     CDS             complement(5947..6183)
                     /locus_tag="PKU75_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79918.1"
                     /translation="MILSPTDRARLGVIRPGDEFDIDADAAGLRPGDDVALTVRRAAG
                     DVIQPALTAAVETTEEATLLRAGGAIPFILRELS"
     gene            6436..6834
                     /locus_tag="PKU75_03240"
     CDS             6436..6834
                     /locus_tag="PKU75_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPF79919.1"
                     /translation="MAVVVGYVPTPEGRAAIDFAIEEARRRDTRLVVLNTSSGNSLVD
                     RRYAGDADLDELNTTLAASGVEFEISQSVTGGKDASDAVIDVAEQVNAELIVIGLRRR
                     TPVGKLILGSQAQRILLEADCPVVSVKATR"
     gene            6940..7203
                     /locus_tag="PKU75_03245"
     CDS             6940..7203
                     /locus_tag="PKU75_03245"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HPF79920.1"
                     /translation="MKTFDQLYAELAAKAVERPEGSGTVAALDAGVHAIGKKIVEEAA
                     EVWMAAEYEGRDKTAEEISQLLYHLQVLMLATGVTPDDVYTYL"
     gene            7217..8062
                     /gene="hisG"
                     /locus_tag="PKU75_03250"
     CDS             7217..8062
                     /gene="hisG"
                     /locus_tag="PKU75_03250"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPF79921.1"
                     /translation="MLRFAVPNKGSLSESAVEMLREAGYRTRRDSKELVVSDVDNDVE
                     FFYLRPRDIAVYVGSGTVDAGITGRDLLLDAGSGAEEVMPLGFGASTFRYAARAGDVT
                     DVAAIAGRRVATSYPGLVTKHLAQAGVQADVVRLDGAVETAITLGVADVIADVVETGT
                     TLRAQGLAPFGEPILKSEAVLIRRPGTSEAQQFEVVRRRLVGVVTARHYVMLDYDCPA
                     ALVDAACRLTPGLESPTVSPMHNPDWLAVRAMVPRAQTNRVMDALYDLGARAILVTEI
                     SACRL"
     gene            8059..8529
                     /locus_tag="PKU75_03255"
     CDS             8059..8529
                     /locus_tag="PKU75_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPF79922.1"
                     /translation="MNAHDTTPAPDAPFVPRRGRRMALSAAVAAIVLFGVIALLLPGP
                     DSGGRWGPLDKLMVWGLGLAIAALMYRYTRIAAWPQEDGLKVRNLMLTQTLPWSHIED
                     VRFGGGEPWVQAEMVEGETIAVMAIQRADGEFGVAEAHRLAALVNRRQGGQAPG"
     gene            complement(8729..9940)
                     /locus_tag="PKU75_03260"
     CDS             complement(8729..9940)
                     /locus_tag="PKU75_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79923.1"
                     /translation="MSTPAPTSRPLAARGLLLLAVLGVAANLRGVIASVPPLAGQIGA
                     DLGLGSGWVGALTALPVLCMGVFAPLAHRLAARLGSATPISAGVLAIAVGSALRAYGT
                     HTWPLYAGTLLAGVGIAIAGTLLPGLVKHLFPPHRAGVVTGAYMLAMMAAAGLASAVS
                     VPLADALDSWALALAVWALPALSGLLLWLPLSRRVHHTAPRVDADDEAAVTHRLPLRH
                     ATAVTLTAYLALQSWQFYSALAWLAPTYVDHDWTRARAGYLLSVFTATQMVAGFVAPA
                     LADRVRDRRLLLLPAVAFALAGELGVLLAPDAAPWVWSALLGLGQGAAFALGLVLLVS
                     YAASPAASARLSAMGLGIGYAVAAFGPTVMGVVRDAAGGFRAVWLVLSVLLIVQVVVT
                     TRLGPDLEPVR"
     gene            10129..11079
                     /locus_tag="PKU75_03265"
     CDS             10129..11079
                     /locus_tag="PKU75_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79924.1"
                     /translation="MRRSSTMLVAGAVTALACGVVAAPAGAESAPPSRPGTQVPVYAG
                     PLDGTTSLIADDTSGKAARTARITVTYHGFNATSKAAFQRAVDMWSTKISSPVPITID
                     ATWAPLGSNILGSAGSNYIWKVGGVWQGDAIANKKAGRQLNSAPDIVARFSSSFTNWH
                     FGTGPAPAGKYDFTSVVAHEIGHGLGFWGVGRVNGALGTVRYNGANGVYDRYTKLGSS
                     TGSPLLWKMPDNSTKLGSALRSNNLYFDSPAVRNANGGKAARLYAPGTWRQGSSYSHL
                     NESTYPKGNPNSLMTYAIGGGETIRTPGNITMAIFKTIGW"
     gene            11181..11954
                     /gene="hisF"
                     /locus_tag="PKU75_03270"
     CDS             11181..11954
                     /gene="hisF"
                     /locus_tag="PKU75_03270"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPF79925.1"
                     /translation="MALAARVIPCLDVDAGRVVKGVNFEDLRDAGDPVEKAAAYGAAG
                     ADELTFLDVTASSAGRDTTYDIVRRTAEQVFIPLTVGGGVRTVDDVDKLLRAGADKVG
                     INTAAIHRPELIAEVADRFGAQVLVLSADVRRLRGPDGQLLGGFEVTTHGGRRSAGLE
                     AIEWCARAAQLGAGEILLNSMDADGTTAGFDIELLKLVRAEVSIPIIASGGAGTPEHF
                     AEALDAGADAVLAASVFHFGTLTIGEVKDAIRAHGHPVR"
     gene            12009..12662
                     /locus_tag="PKU75_03275"
     CDS             12009..12662
                     /locus_tag="PKU75_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HPF79926.1"
                     /translation="MTSSSTPRFAQVERGALCDTFLSVGPDAPTLCEGWTTADLATHL
                     VVRDGRPDLIVGPMLPVIGGVAKGQVRAIKHQPWPDLVEAVRSGPPRLSPVRIGKVDE
                     LVNHVEYFIHHEDVLRAQPGALRREIPDAEQRALWRALARMAKLMFRKSAVGAVLIAP
                     QGRVVAKAPTDLGSVDIAGRPGELLLAAYGRHAQADVTTTGGDQAVAALWSSKLGLA"
     gene            12672..14591
                     /locus_tag="PKU75_03280"
     CDS             12672..14591
                     /locus_tag="PKU75_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79927.1"
                     /translation="MPVDDLGAGAGSDRSLRRALSVIALGVRAEPRLFAIAVTGSVLY
                     GVLTAGMAQAVGWVTAHQITSAIDEGGLSRGRALIIGSVIGGVVLLTTVGVLLRRIGG
                     GFTVFNVGADYRRKVTRQYLRLPLAWHHRHPSGQLLSNAHADVEATWNIFMPLPMSLG
                     VLVMLLAGAVQMVLIDPVLAGVGLVIFPLLFAANAAFQRRMSERVTRAQQLRAEVAEV
                     AHESFEGALLVKVMGREQRETSRFAEVSDRLRTAAIRAGRTRGTFDPVIEAIPTFGTL
                     AVLGVGVGRVASGAVTTAELVQLAYLISVLAFPVRAFGWVLGEIPRTLVGWERVNAVL
                     QARGSMSHGAADLGQGSPGARLTMDGVTYSYEAASDAALTGSGATAGSADLPASGALG
                     APIALRDITLDVAPGETIAIVGPTGAGKSTLAGLIVRLIDPTSGRVSIDGVDVRDLAP
                     GALAADAALVPQSTFIFDDTVRGNVTLGAPVPDAQVWAALRTAHADGFIARLPDGIDT
                     KVGERGATLSGGQRQRIALARALVRHPRLLVLDDATSAVDPTVEQAILAALGAGTGAM
                     TVVVIAYRMSTIALADEVAFLDEGRLVDRGTHAELMSRCQGYADLVTAYAREAAERAA
                     VAADEEPSDPVEVTS"
     gene            14618..16387
                     /locus_tag="PKU75_03285"
     CDS             14618..16387
                     /locus_tag="PKU75_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF79928.1"
                     /translation="MQTKPAGGAWATLKRGVSLSPELTKGIGVTLLLAIVTTAGRVVV
                     PVVVQRVTDDGLLAAGGPDAGIVYRYLAIAAVVVVITAICSYAVNVRLFTASEGGLAT
                     LRVKAFRHIHDLSVLTQNAERRGSMVARVTTDVDTISQFVQFGGIVLIVSLGQITLAT
                     VLMVIYSPVLAAVTWGCFIPLVIAVRRMQKSLGAAYGDVRERVGDLLAAVSESVVGAA
                     TIRSFGVADRTAERLDAAIEGHRKAATTAHIRSTIAFSAGQFVGGITLAVVLVVGTWL
                     AARGDMTLGELLAYLFLVGLFTQPVQQATEILNELQNAVACWRRVIQVMDTPADVADP
                     GTDGIDLPRGPITVDFDDVGFAYPGGPPVLHDITEHVAPRSRIAIVGETGSGKSTFAR
                     LLTRLMDPSSGIVRLDGVDIRDVRFGSLRSRVVMVPQEGFLFDDTVLENIRFGGEHSR
                     ADVELAVTELGLDAWLSGLPAGLDTQAGQRGESLSAGERQLVALARAYLADPDLLVLD
                     EATSAVDPATEVRIQRALDSLARGRTSIAIAHRISTAQAADEVLVMDAGRVVQRGPHA
                     RLVTEDGPYAALYASWVANQGAH"
     gene            16485..16859
                     /gene="hisI"
                     /locus_tag="PKU75_03290"
     CDS             16485..16859
                     /gene="hisI"
                     /locus_tag="PKU75_03290"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPF79929.1"
                     /translation="MSLDPAIAARLKRDAQGLVAAIAQQHDTREVLMLGWMDDEALHR
                     TLTSGRVTFWSRSRGEYWRKGDTSGHAQIVKSVALDCDGDALLVTVDQIGAACHTGDR
                     TCFDAGDLGATISPDAPAGGEA"
     gene            16856..18409
                     /locus_tag="PKU75_03295"
     CDS             16856..18409
                     /locus_tag="PKU75_03295"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HPF79930.1"
                     /translation="MSAGPATLPYGSTWPALETFTVLAQDRRVVPVVRRLLADGETPL
                     ALYRKLAQGKPGTFLLESAEHGGVWSRYSIIGAASRATLTERDGEAVWIGEPPVGVPA
                     GGSPVDAVRDTVAALATEALAGLPPLTGGLVGAISYDAVRRWERLPEKAIGDLDLPEV
                     AMVLATDVAVLDHSDGSVLLIANAVNYDDTDERVEWAWTDAVERLDAMTAALATPTDS
                     TVAVLDAARADTALENVRSNLTQEHFEDLVEQCKEDIRAGEAFQIVLSQRFDIDCPAD
                     GLDVYRVLRSSNPSPYMYFFRLDHPASGSFDIVGSSPEVHLKVTGREVILHPIAGTRP
                     RGKNPEHDLLLEEELLADPKERAEHVMLVDLGRNDLQRVCYAGTVDVVEFLNIRHYSH
                     VMHIESTIVGQLAREATAYDALAATFPAGTLSGAPKPRAMALIEEYEPTRRGIYGGVV
                     GYFDFHGDMDVAIAIRTAVITQGKAFVQAGAGIVADSVPLSEYEETRNKAAAVLRAVA
                     AAGTLGPPR"
     gene            18406..19026
                     /locus_tag="PKU75_03300"
     CDS             18406..19026
                     /locus_tag="PKU75_03300"
                     /inference="COORDINATES: protein motif:HMM:NF021079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HPF79931.1"
                     /translation="MSRMRWTGPRVVVPVALLAAGLAAISGRRVWLTGRVEDAVVGSS
                     TVTGTGTQVVPGLVALALVLAAAVLAAATAGAVMRRLALVLGAVAGIALAVLAVRASS
                     SGASLLGGLAAEASSRTGTIPVTGVSVGSWPWLVAAAATAGATACLAGLAGASQWRGL
                     SQRYDAPAAEPAGAGPRGQRTTSAWNDLDDGVDPTQAPGTPPGDPL"
     gene            complement(19098..19511)
                     /gene="arfB"
                     /locus_tag="PKU75_03305"
     CDS             complement(19098..19511)
                     /gene="arfB"
                     /locus_tag="PKU75_03305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HPF79932.1"
                     /translation="MPPGPGLPAGLVVPGELLRERFSRSRGPGGQGVNTTDSRVEVEL
                     SLADASWLTETQRSRAVAALGDVVTVAASEHRAQRRNRVAARERLAALLREALAPPPP
                     ARRATTPTRGSRERRIAAKRRRAQVKAQRTRPAPD"
     gene            19663..19893
                     /locus_tag="PKU75_03310"
     CDS             19663..19893
                     /locus_tag="PKU75_03310"
                     /inference="COORDINATES: protein motif:HMM:NF041681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HPF79933.1"
                     /translation="MSTHEDEHDSHGNSTAAWVSTMIMLVAAAVICWAIIQANTSLAI
                     AGGVAFLIGALLWPILAKMGYGEKNPVRSPYA"
     gene            19967..20776
                     /gene="trpC"
                     /locus_tag="PKU75_03315"
     CDS             19967..20776
                     /gene="trpC"
                     /locus_tag="PKU75_03315"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPF79934.1"
                     /translation="MPANATVLDSIIAGVRDDVARREDRMPLREVVAAAAAAPPARPV
                     LPRLTRSTLGVIAEVKRSSPSKGALAEITDPGSLAAAYREGGAAAISVLTEQRRFGGS
                     LADLDAVRAAVEVPVLRKDFMVCDYQFHEARAHGADLVLLIVAALTDDELRRFHDLTH
                     ELGMTALVEVHDEAEIDRALAVAPRLLGVNARNLKTLDIDADAFARLVTQVPDDVVAV
                     AESGIAGPADAQRYAREGARAVLVGEALVKDGDPRAAVAAMAEIPTGATHV"
     gene            20814..22019
                     /gene="trpB"
                     /locus_tag="PKU75_03320"
     CDS             20814..22019
                     /gene="trpB"
                     /locus_tag="PKU75_03320"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPF79935.1"
                     /translation="MSDAPGRFGRFGGRYVPEALIPALEQLDEVRQKAMVDPDFQAEL
                     DHLHKTYTGRPSIITEVPRFAAHAGGARVILKREDLNHTGSHKINNVLGQALLTRRMG
                     KRRIIAETGAGQHGVATATAAALMGLDCTVYMGKVDTERQALNVARMKILGAEVIPVE
                     HGSATLKDAINEALRDWVTNVHDTHYCIGTVTGPHPFPEMVRDFHKIIGIEARQQVLE
                     LTGRLPDAVIACVGGGSNAMGIFHRFVEDEGVRLIGVEAGGEGVESGRHAARFASAEP
                     GVLHGAMSYLMQDDDGQTLESHSVSAGLDYPSVGPEHSHLKDIGRAEYRYATDEETME
                     AFRLLCRTEGIIPALESSHALAGALDVGRELGPDGLILVNLSGRGDKDMHTAAAWFGL
                     IDAQGEGTV"
     gene            22016..22834
                     /gene="trpA"
                     /locus_tag="PKU75_03325"
     CDS             22016..22834
                     /gene="trpA"
                     /locus_tag="PKU75_03325"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPF79936.1"
                     /translation="MTPATAVGSAAVFETCRQEGRAALVGYLPVGFPDVATSIRAMVT
                     LVENGCDIVEIGIPYSDPVMDGPAIQRAASAALAGGTRVEDVFAAARAVRDAGAVPLV
                     MTYWNLVLRRGVDRFADDLAAAGGGGLITPDLIPDEAGDWIAASDRTGLDRVFLVAPS
                     STPERLATVTGACRGFVYAASTMGVTGVRSSVGTAADELVERTRAATDLPVCVGLGVS
                     TGEQAAQVGSFADGVIVGSALVTTLLDVEPEAGLARLAEVTRELAAGVRESKRS"
     gene            22966..23763
                     /locus_tag="PKU75_03330"
     CDS             22966..23763
                     /locus_tag="PKU75_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPF79937.1"
                     /translation="MSKKPTAQRGSRAGATPQDSRQAKIQAAQKASGSSANKITIAGI
                     VAILAIIAVVGGVIWNEKNNEKEAGSGNTAPPAGATVDAGYRSFADVTLASGAPVVTV
                     FEDFQCPACKQYEAAMGSTLAQLAKDGKIDLRYHIMNFLDDKTGAKQSTPVANGAFCA
                     ADQGKFQEFHDAAYTAQHPEGDSVTTADVSKFATTAGVGDMDAWTKCVDAGKYTSYIN
                     ASNESAGTAGVTGTPTLKIGDKLYSGETLGEVATADGLTKVVEAATK"
     gene            23834..24718
                     /locus_tag="PKU75_03335"
     CDS             23834..24718
                     /locus_tag="PKU75_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPF79938.1"
                     /translation="MAVTPPPPQDRSPAGRGAEPRPPAGASRGAVAAIVALVTVVAVA
                     AGVIWSQRSGQDQAGPGASVPASGTASRTEEPVPTASAAEATAPTAGNTTSTASASAS
                     GPQSTPEGAGVDEGYRSFAGVDLRPGAPVVTVFEDFQCPACAQLEGAIGATLNELAAD
                     GKIQLNYRIMNFLDEMTGGTQSTPVANGAFCAADQGKWQEFHDAAFAEQYPEGTSVSV
                     EQIAAFARTAQVPDLDAWTACTESAPYSTFVADSNAAATTDGVTGTPTVWIDGKPVAL
                     RDIITPAGLRAAIVDATE"
     gene            24725..25591
                     /gene="lgt"
                     /locus_tag="PKU75_03340"
     CDS             24725..25591
                     /gene="lgt"
                     /locus_tag="PKU75_03340"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPF79939.1"
                     /translation="MITYIPSPTVGALHLGPLAIRGYALCILTGIVVAVWLAGRRLEH
                     RGGKRTDALDIATWAVPFGIVGARIYHVLSSPEAYFGADGRLIDALKIWQGGLSVWGA
                     IALGALGAWIGCRRAGVGFLDFADSAAPGIAIAQALGRWGNWFNNEVYGERTDVPWAV
                     QIHQWDTATGSAVKDASGDPVVLGTFHPTFLYEAIFCLLLAIVLLMLDRRRAFAPGQL
                     FALYVAGYPTFRIVLEKMRTDEATIILGQRINVWTSIAVFLLGVGLYAWTGRRSRRST
                     PVSQGEKSLTTS"
     gene            25676..>26455
                     /locus_tag="PKU75_03345"
     CDS             25676..>26455
                     /locus_tag="PKU75_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HPF79940.1"
                     /translation="MASALFSAQPADHGLYRREFEHDACGVAMVATLRGHAGHDIVVQ
                     ALTALTNLDHRGATGADPLVGDGAGILTQVPDAFLREVAGFTLPPRGSYAVGAAFLPA
                     DPEARSEAVHRIEDLAHEEGLEVIGWRDVPVTPDLVGTAARDCMPHFAQLFLAASDGA
                     VRGIHLDRRAYCLRKRAEREADVYFASLSARTLVYKGMLTTGQLEPFFPDLSDERFVT
                     ELALVHSRFSTNTFPSWPLAHPYRLIAHNGEINTVKGNRNWM"
BASE COUNT         3925 a         9590 c         9147 g         3793 t
ORIGIN      
        1 acaacgccga cgacgcagcc gccaatatcg cccacctgcg cgaacttgtt gcggcacacc
       61 cacattgaca gcgagcctga tccgcggtcg tgtgctaggc caggggcagg tgcggtgtcc
      121 catcgacgtg cgcggcgacg gggatggatg ggtatgccgc ccggtcccct cgacgtgttc
      181 gggatcgagg ttctcgccga ggacgcacgc cgatgcctag ggccgtaggg actttacgaa
      241 acccatgacc tgagatcgga ctttggacgg cccgtcgtcc acgtcataga gcagctgttg
      301 cgcgtggccc tctccggggg ccgacacgta cgtgaccagg gtgggagcct catcgacggc
      361 ggcccggacc cggtcctcat cggccatcca ctcgactcgg cggcccaggt gcacagcccg
      421 cgtcgtcccg atccgtggat gaggacgacc ccgagcgcgc cgcgtcccgc tgcgacgccg
      481 taaaggcggt ccggcccgct gcccatccac aggggcgtcg acgcgggcag cggggtgccg
      541 cagcggtcgc cgagatccgg agtctccgtg gcctgcgtgg cacttcccgg cgccggctgg
      601 gtgggtgcag tgctgtcggc gagcgtcgac gcggaggttg acaccggcga aggtgtcgat
      661 gacgaccccg aatccggcga cccgcacccg gccaccagca ccagcgcgcc acagcagaca
      721 atgacgtgcc agtagcggtg agcccgattg ggttgcacct gtccatcctg cccggaccgg
      781 cagccattgg cgcgggctcg tcgtggactg ctccaccaac cggccaagag cggcatcacg
      841 tggtgcgctc ggcctcggtc aggtgcgggg tccagttgat ccgcacggca acgggtgtgt
      901 tgtcgtaggc cgcctgctct tcgagcaggg tcaccgaggt gttgtcgatg tcgatccagc
      961 cgccgatcgt gtcgatgctc aggaaggcgc gcaggagata ctgcgtgaag tggccgtggg
     1021 tcacgagcgc gacggcgtcg cgagccgcgg accctccggc agtgcccgag tccgtcggcc
     1081 agaggctggt atgccgctgg cgcacctcct ccagaacgcg cgtcgcgcgg gccaaccgtc
     1141 cgtcagcatt ctcgacatcc cctctccacc aaccgctttc gtcgacatcg tcggcgagtt
     1201 ccagtctccg cgagaggccg agcagcgcgg tgcgaccggc cccggcatgg ggtgtgcggg
     1261 cgccggtggc ggggtcctcg tcatacggcc cgtcggactc gaagatgtcg agatggcgca
     1321 agggcaggtc cagggcatcg gcgagcggcg cggcggtctg cacggcgcgg gtcatcaggc
     1381 tgcagtagag cgccccgatc cgccagggca agacaccgtc cgcgacggcc gcagccagct
     1441 ttcgcgcctg ctcatgtcca cggtcggtga gcggggtatc cgggtgccgg cccacggagc
     1501 cgccggtttg ctcccagatg aggttgttgc cggactgccc gtgacggatc aggatcagac
     1561 gcacgattca catcccacca catgccgtcg cggacaccgc ctcatccggc gcagacgaga
     1621 gcgcgcaagt ccgccgcagt ccggcgcagc cggtcggccc ggtccggatc gagcgactcc
     1681 acttgcgccg cagcggcatc cgcttcgtcg gcctccgcct ggacgaggga gcgctgctct
     1741 cgctcggaca ggtcgcgccg agctgcatca gccgccccga cgccgagagc ggcgttctcc
     1801 accgcacccg cccggaagtc ctccgtgagc ggcaccgctt cggcgttctc gatccgtgcg
     1861 agcgccgatc gcagcgcact cacccgctct cggtcgcggg ccttcatcgc cacgccgagt
     1921 gcggcgcgca gccgggcgcg gaccggagcc gtcggggaag tcacccgagg gaatctacgg
     1981 cgtgatgacg accttgtgca cgctcttatc ggaccgcgga gcctccacgc acctgctgtt
     2041 ggggttgacg ggccaccgaa ccttccggcg tcgaatcgtg accggaccag ggagaggtga
     2101 ggctcgccat acgggcaggt gaggacgggt gggcgcaagg gtggtattag taggaccacg
     2161 tgctccgggg tcggtgtaat tccgaaccgg tggtgatagt ccacgacccg ttggcagcca
     2221 gtcagcggtg gacccggtgt aattccggga ccgacggtga aagtccggat ggtaggcagc
     2281 acgcaaccga aggcgggtcc gccccgtcgg cgtcagtagg cgtcgtcgcg cggctcccgc
     2341 ctgaaggtgc aggtcccccg gagttcgtcc agcgatagca ccgaacaccg aaggacgaac
     2401 accacatgtc atcgctcgtc atggccgccg ccgcggacca gaacatcttc cagcggctca
     2461 tcgaggccgc catccccatc gggaaatacc aactgcatta cctcgagttg atcggggtcc
     2521 tcatcggcgt cgtctccgcc tatttcggca tgaaacgccg cgtctgggcg tggccggtcg
     2581 ggatcaccgc caacatcatg ctcttcttcg tctacatcgg ggccctggtc gccgcggacg
     2641 aacgcatccc gctgttcggg caggccgggc ggcagatctt cttcatcgcg accagcatct
     2701 atggctggtg gcggtggaac gccatccgca aggcacgggg tgcggccagc gacgccccgg
     2761 ccatcacgcc tcgctgggcg accacccgtg agcgcatcgc gctggtcgtc ggttggctgc
     2821 tcgccaccgt cgtcgcccac caggtcttcg tctggctgtg gaacctctcg cccaacccgt
     2881 actggacccc gcagtggtgg tactactggt gcgacgcctg gatcttcgtc ggcagcttca
     2941 tcgcgacctt cgccatggcc cgcggctgga acgagttctg gctggcgtgg atcggtgtcg
     3001 atctggtcgg cgtgccgctc gggttcgcga cgaactacgt cccgaccgcg gtcatgtact
     3061 ccttctacgg gctcttcgtg atctatggct tcacccagtg ggtcaaggcc tcccgccagg
     3121 aggccccgac cgcccaggac cgcgaggccg tccccaccgc cgctggttag ccgccacgtc
     3181 gaggagggtg aggtcgaagg acgccgaggt cagctcgcta cccagaaggt gcgggtgttg
     3241 gtgaaggcga gcaggccggc gtcggcgagt tcgcggccat accctgatcg cttggtgccc
     3301 ccgaagggca cgcgtgggtc gctggcgacg atcgcgttga cgaaggccgc accgctggtg
     3361 atgcggcggg ccacggcaac cgcatgttcg gtcgcgccgc tccacaccga caggccgagg
     3421 ccgtagtcgg tggagttggc gagttcgacg gcgtggtcgt cgtcacgggc cacggtgatg
     3481 gctgcgacgg ggccgaaggt ctcctcgtcg aaggcggcca tgccaggagt gacttccgac
     3541 cgggccgatg cgcaggccgt ccggcagcca cgccgtctat caggagttgc gccggcgaat
     3601 cctccaggag gagctggctc cgggtgagcg cttgcacgaa tcggcgctcg cggccgatct
     3661 cggggtcagc cgcaccccct atcgggaggc cgtgcgcctg ctcatggccg aggggttgct
     3721 cgaccagctc ccgacaggcg gagtcgtggt ccggggggtc tcggcccgtg acatcgagga
     3781 gctctatgcc gtgcgtgccg cattggaggg gctgatggcc gcctgcgccg ccactcgcgt
     3841 cgacgccgcc gcggtcaccc gcctcgaatc gcttgtggaa cgcaatgcgc gcctcgtcga
     3901 tctcccgcag gaggccgcgg aagcgggcca cggactgcat ctggccatcg ccgagatcgc
     3961 cggaaacgat tgggccacga agctgcacag ccaggtcgat gcccagatgt cgcggtatcg
     4021 ccactacacc aacgagaccc agaaacgccg cgaagccgct ctggatgagc accggcggat
     4081 cgtggaggcg ctgcgcacag gcaacgccgc gactgcccga tccgcggccg agtcccatgt
     4141 tctcgccgct cgggacaccg ccctcgcctc gatcgccgga cggctgcgcg cccagcgact
     4201 ctgaggcgac gttcccgccc cacgcgccga caccgggtac ctatgatccg ggcatggcag
     4261 tagagctgat cctggtccga cacggccagt ccgagggcaa tgtcgcggcc gaggcggcag
     4321 atcgcgaccg gctcgagcag atcgcggcgc cggcccgcga cccggacgtc atgttgtccg
     4381 agaccggccg cacccaggcc gcggccgtcg gcgcctatct cagcgccctg cccgcggacc
     4441 agcggcccgg catcgtgtgg accagccctt atcagcgagc gcgggagacg gccgagatcg
     4501 cgctgcatgc gtatgacgcc gaactcgact tccgcgtcga cgagcggctg cgcgaccgcg
     4561 acatgggcat caccgacatg ctgaccgggg caggcatccg ggcgaagtat ccggaggagg
     4621 ccaagcgccg ggcgtggatc ggcaagttct actaccgccc gccgggtggt gagtcatggg
     4681 cggacgtggc cttccgggtg cgctcggtcc tgaccgatct cgccaacagc gagaagcacg
     4741 atcgcgtcct cgtcaccgcc cacgatgtcg tcatcctgct cttctgttat gtcgccgaag
     4801 gcatggacga gaaggcggtg ctcgagcgcg ccaccaccaa tgggttgcgc aatgcggcca
     4861 tctgccggat ccgccgcgac gaggactccg cgaccggctg gaccgtcacg gcatacaacg
     4921 aggactccca cctccgcgac gccggggtgg cggtcaccag ccagcccggc gcctccgacg
     4981 aggtgggggc gacgtgagcg ggtccccgag gggtatcccc gagcacgcga gcgctgggac
     5041 ggcagacgca ccgcgtctgg tcaccccagc gctgctgcgc gaatggccgc tgccggagcc
     5101 gacggggacc aagctcggcc gcggccgggt ggtggtcgtc ggtggcgccc gcaagacgcc
     5161 cggcggcgcg atgctcggcg gcctggcggc gttgcgtatg ggggccggcc gcctcagcct
     5221 cgctgtcgcc gaaagcgttg cagtgcaggt ggctgtggcg atcccggagt cgggcgtgca
     5281 tgggctgcgc gagtcggcgt ccgggtccgt caccggggcg ggtgcagccg gcatcctcgc
     5341 gccggaactc gagcacgccg atgggctgct cgtcggcccg gggctggacg atccggccgg
     5401 caccatccgc ctgctcgagg aggtggtgcc ggtgtatgcc gccgaccggg ccatcgtcct
     5461 ggacgccttc ggcgcgacgg ttctccccga tgtcgccgac ggctgcagga aggcgttggc
     5521 gggtcgaaca ttgttgaccc ccaacacgaa tgagctcgcc cggctggcga ggcgcacgga
     5581 gttggacgag gacgatgtgc ccgctgctgc gctggaggtg ggggaggagt tcggcgcgat
     5641 cgtcagttgc ggcggctgga tcaccgcgga cggtggtgtc tggcgcatca ccaccggcga
     5701 cacgggactc gggacctccg gcagcggtga cgtgctcgcc ggtgccgtcg tcgggctggt
     5761 ggcccgcggc gtggccccct tgccggcgat gctgtgggct acctacatcc acgcttcagc
     5821 gggtgaccgg ttggcgacgg agttcggtcg cgtgggtttc cttgcaggag aactgctccc
     5881 gcaactgccg cgggcgttgc ggttgctgcg cggggactga gtcggcaggc tcctccgacg
     5941 gcggtgtcag gataactcac gcaggatgaa ggggatggcg ccgccggccc gcagcagggt
     6001 ggcctcctcg gtcgtctcca ccgccgccgt tagggcgggc tggatgacgt cacccgcagc
     6061 acggcgaact gtcagcgcga catcgtcgcc cgggcgaaga ccggccgcgt cggcgtcgat
     6121 gtcgaattcg tcgcccggcc ggatgacgcc gaggcgggcg cggtcggtgg gggagaggat
     6181 caacggggca ggatcctcac cacgtcatcg acggggatgc tgaggcggcc gaggaggcaa
     6241 tgcgccgcca catcgctggc agccgcggag ccaaggcgct cacgcgccgg cgctgggaca
     6301 gggaccgggg tcagagcgga ctgtagcgcc gctcggggcg gccggtcgtg ccgatcgtca
     6361 tcgagcgccg ccgcgccgcc catggccagg ctccgctcga ggtcagccac gccaatatcg
     6421 aaggaggcaa ctgatatggc cgtcgtcgtg ggctatgtgc ccacgcccga agggcgcgcc
     6481 gcaatcgatt tcgcgatcga ggaggcccgc cgccgcgaca cccggctggt ggtcctcaac
     6541 accagcagcg gcaactcgct ggtcgatcgc cgctacgccg gtgacgcgga cctggatgag
     6601 ctgaacacca cgctcgccgc ctccggcgtc gagttcgaga tcagtcagtc ggtgacgggc
     6661 ggcaaggacg cctcggacgc cgtgatcgat gtcgccgaac aggtcaacgc cgagctcatc
     6721 gtcatcggcc tgcgccggcg caccccggtc ggcaagctca tcctcggcag ccaggcccag
     6781 cgcatcctgc tcgaggccga ctgtcccgtc gtgagcgtga aggccacgcg ctgaccacgc
     6841 ggcagagcgc cgcaccaaaa aaacctaaca gagcgccgtc gtccacctcg tgggcggcga
     6901 cgttcggcat gtcggtggtc gcgcttaccc ttggtgcccg tgaagacctt cgatcagctg
     6961 tatgccgagt tggccgccaa agcggtcgag cgtccggagg gttcgggcac cgtcgccgcc
     7021 ctcgacgccg gcgtgcatgc gatcggcaag aagatcgtcg aagaggcagc cgaggtctgg
     7081 atggccgcgg aatacgaggg tcgggacaag acggccgaag agatcagcca gctgctctat
     7141 cacctgcagg tcctcatgct cgccaccggc gtcacccccg acgacgtcta cacctatctc
     7201 taggacgggt tttcgtatgc tgcgcttcgc cgtccccaac aagggttccc tttcggagtc
     7261 cgcggtcgag atgctgcgcg aggcgggata tcgcacccgc cgcgacagca aagaactggt
     7321 cgtctccgac gtcgacaacg acgtcgagtt cttctacctc cgcccccgcg acatcgccgt
     7381 ctatgtcggc tcgggcaccg tcgacgccgg gatcaccggc cgcgatctgc tgctcgacgc
     7441 cgggtcgggg gccgaggagg tcatgccgct gggcttcggt gcctcgacct tccggtatgc
     7501 cgcccgcgcc ggcgatgtca ccgacgtcgc cgcgatcgcg ggccggcggg tcgccacgtc
     7561 atacccgggg ctggtcacca aacacctggc gcaggcgggg gtgcaggcgg acgtggtgcg
     7621 cctcgacggg gcggtcgaga ccgccatcac gctcggcgtg gccgacgtca tcgccgacgt
     7681 cgtggagacc ggcaccacac tgcgcgcgca ggggctggcg ccgttcggcg agccgatcct
     7741 gaagtccgag gccgtcctga tccgccggcc cggcaccagc gaagcgcaac agttcgaggt
     7801 cgtccgacgc cggctcgtcg gcgtcgtgac ggcgcgccac tacgtcatgc tcgattacga
     7861 ctgccccgcc gccctcgtcg acgcggcctg ccgactgacc ccgggcctgg agagcccgac
     7921 ggtctcgccg atgcacaatc ccgactggct ggccgtgcgt gccatggtcc cgcgggccca
     7981 gaccaaccgg gtcatggatg ccctctacga cctcggtgcc cgggccatcc tggtcaccga
     8041 gatcagcgcc tgccggctat gaacgcgcac gacacgaccc ctgcccccga cgctccgttc
     8101 gtcccgcggc gcggccgccg catggcgttg tccgccgccg ttgccgcgat cgtgctgttc
     8161 ggtgtcatcg cgctcctcct gcccggcccc gactccggcg gccggtgggg gccgttggac
     8221 aagctcatgg tctgggggct cgggctggcc atcgcggcct tgatgtatcg ctatacccgc
     8281 atcgccgcct ggccccagga ggacgggctc aaggtgcgca acctgatgct cacccagacg
     8341 ctgccctggt cgcacatcga ggatgtccgc ttcggcgggg gcgagccgtg ggtgcaggcc
     8401 gagatggtcg agggcgagac gatcgccgtc atggcgatcc agcgcgccga cggggaattc
     8461 ggtgtggcag aagcccatcg gctcgccgcc ctggtcaacc gccgacaggg cgggcaggcg
     8521 ccgggctagg cgggctagtc ggcggcgtgc ccgcttgtcc gcggggtgtc acgagatggg
     8581 gtatccaagc ggccgcatcc cccgactccg gacacgagga ccgcgtcggt gtcacgacgt
     8641 gggcgatcgc ggcgggatca tcgcccatct tgtgacagac gggcggttcg gccgccgagc
     8701 ggcgcgacaa cccgccgcgc agcctgggtc agcggaccgg ctcgaggtcc gggcccaggc
     8761 gcgtggtgac gaccacttgc acgatcagga gaacgctcag caccagccat accgcgcgga
     8821 acccgcccgc ggcgtcgcgc acgacaccca tgaccgtcgg gccgaaggcg gccacggcat
     8881 acccgatccc gagacccatc gccgacagcc gcgcgctcgc ggcgggactc gcggcgtagc
     8941 tcaccaggag caccagcccg agcgcgaacg cggcaccctg gccgagaccg agcaacgcgc
     9001 tccataccca gggcgcggcg tccggggcga gcagcacccc cagttcgccc gcgagggcga
     9061 acgcgacggc cggcaggagc agcagccggc gatcgcggac ccggtcggcg agcgccggcg
     9121 cgacgaatcc cgccaccatc tgcgtggcgg tgaacaccga cagcagatag cccgcgcggg
     9181 cccgggtcca gtcgtggtcg acatatgtcg gggcgagcca ggcgagcgcg gagtagaact
     9241 gccacgactg cagcgcgagg tatgccgtca gggtcaccgc ggtcgcgtgg cgcaggggga
     9301 ggcggtgtgt caccgccgcc tcgtcgtcgg cgtccacccg aggagccgta tggtgcacgc
     9361 gccgggacag cggtagccac agcagcagcc cggagagcgc gggcagggcc cacacggcga
     9421 gggcgagggc ccaggagtcg agggcgtcgg cgagcggcac gctgaccgcg gaggcgagac
     9481 cggcggcggc catcatcgcg agcatgtacg cgccggtgac cacgcccgcc cggtgcggcg
     9541 ggaacaggtg cttgaccagg ccgggcagga gggtcccggc gatggcgatg ccgaccccgg
     9601 cgaggagggt cccggcatac agtggccagg tgtgggttcc gtaggcgcgc agcgcggagc
     9661 cgacggcgat ggcgagcacc ccggcggaga tcggcgtcgc cgacccgagg cgcgcggcga
     9721 gccggtgggc gagcggcgcg aacactccca tacacaacac cggcagcgcc gtcagcgcac
     9781 ccacccaccc cgagcccagc cccaggtcgg cgccgatctg acccgccagc ggcggcacgc
     9841 tcgcgatgac cccgcgcagg ttcgcggcga ctccgagcac cgcgagcagc agcagcccgc
     9901 gggcggccag cggccggctg gtgggggcgg gggtggacat gggccccatt gtggccggag
     9961 cagcaggggt cgccggtcgc gcggggcagt ggcggacggc tgcacctcgg gtgttagcct
    10021 cgacgggcac gcccggcaag ggatccggtg cggtgcaagc ccgccgtcgg gcagggtttt
    10081 gccagcccgc ggcagaggct gcggcagatc gaggggaaaa gatacccaat gcgtcgttca
    10141 tccaccatgc tcgtggccgg agccgtcacc gcgctcgcct gtggcgtcgt cgccgccccg
    10201 gccggcgccg agagcgcgcc gccgtcgcgc ccgggcacgc aggtcccggt ctatgccggc
    10261 ccgctggacg gcaccacgtc gctgatcgcc gacgacacct cgggcaaggc ggcccggacc
    10321 gcccggatca ctgtcaccta tcacggcttc aacgccacct cgaaggccgc cttccagcgc
    10381 gccgtcgaca tgtggtcgac caagatctcc tcgccggtgc cgatcaccat cgacgccacc
    10441 tgggcgccgc tcggctccaa catcctcggc tccgccgggt cgaactacat ctggaaggtg
    10501 ggcggcgtct ggcagggcga cgcgatcgcc aacaagaagg ccggccgcca gctcaactcg
    10561 gcgcccgaca tcgtcgcccg cttcagcagc agcttcacca actggcactt cggcaccggc
    10621 ccggcccccg ccggcaagta cgacttcacc agtgtcgtcg cgcacgagat cggccacggc
    10681 ctcggtttct ggggtgtggg ccgggtcaac ggggccctgg gcaccgtgcg ctacaacggc
    10741 gccaacgggg tctacgaccg ctacaccaag ctcggttcct ccacgggcag cccgctgctg
    10801 tggaagatgc ccgacaactc cacgaagctc ggctccgcct tgcgcagcaa caatctgtac
    10861 ttcgacagcc ccgccgtccg caatgccaac ggcggcaagg cggcccggct ctacgcgccc
    10921 ggcacctggc ggcagggcag cagctacagc cacctcaacg agtcgaccta tcccaagggc
    10981 aaccccaact cgctgatgac ctacgcgatc ggcggcggcg agacgatccg cacgcccggc
    11041 aacatcacga tggcgatctt caagaccatc ggctggtaac cagagggctc agcatcggtc
    11101 gagaggggaa cgtcggcacg gtttcgtcca ggatccgtgc cgatcgtccc ctctcgaccg
    11161 tcgctgtcta ggctcctgcc atggctttgg ctgcgcgcgt catcccgtgc ctcgacgtcg
    11221 acgcggggcg ggtggtcaag ggggtcaact tcgaagacct ccgcgacgcc ggcgaccccg
    11281 tcgagaaggc ggcggcgtat ggcgcggcgg gcgccgacga gttgaccttc ctggacgtga
    11341 cggccagcag cgccggacgc gacacgacat acgacatcgt ccgccggacg gcggaacagg
    11401 tcttcatccc gctcaccgtg ggcgggggag tgcgcacggt cgacgacgtc gacaagctgc
    11461 tgcgcgccgg cgccgacaag gtggggatca acacggccgc catccaccgg cccgaactca
    11521 tcgcggaggt ggcggaccgg ttcggggccc aggtgctggt gctgtcggcc gacgtgcggc
    11581 ggctgcgtgg gccggacggt cagctgctcg gcgggttcga ggtgaccacg cacggggggc
    11641 gtcggagcgc ggggttggaa gcgatcgaat ggtgcgctcg cgcagcgcaa ttgggcgccg
    11701 gcgagatcct gctcaactcg atggacgccg acggcacgac cgccggtttc gacatcgaac
    11761 tgctcaaact cgttcgtgcc gaggtgtcga tcccgatcat cgccagcggc ggcgccggga
    11821 ctcccgagca tttcgccgag gccctggatg ccggcgccga cgccgtcctc gcggcgtccg
    11881 tcttccactt cggcacgctg acgatcggcg aggtcaagga cgccatccgc gcccacggcc
    11941 accccgtccg ctgacagctg actcggccgc ccatggcctt gtcggaggga ggaggcagga
    12001 taggcggtat gacgtcttcc tccactcccc gcttcgccca ggtcgagcgc ggcgccctgt
    12061 gcgacacctt cctgtcggtc gggcccgacg ctccgacgct gtgcgagggt tggacgaccg
    12121 ccgacctggc cacgcatctc gtcgtccgcg acggccggcc cgacctgatc gtcgggccga
    12181 tgctgccggt gatcggcggc gtcgccaagg gccaggtgcg cgcgatcaag caccaaccgt
    12241 ggccggacct ggtcgaggcg gtgcgctcgg gaccgccgcg gctctcgccc gtgcggatcg
    12301 gcaaggtcga tgagctggtc aaccacgtcg aatacttcat ccatcacgag gacgtgctgc
    12361 gcgcccaacc gggtgccctg cgccgcgaga tccccgatgc cgagcagcgg gccctgtggc
    12421 gcgcgctggc gcgcatggcc aagctgatgt tccgcaagtc cgccgtcggg gcggtcctga
    12481 tcgccccgca gggccgggtc gtcgccaagg cgcccaccga cctggggagc gtcgacatcg
    12541 cgggccggcc cggcgaactg ttgctcgcgg catacggccg gcatgcccag gccgacgtca
    12601 ccacgaccgg cggcgaccag gccgtggccg cgttgtggtc gagcaagctg gggctcgcgt
    12661 gatcgcacgt catgcccgtt gatgatctcg gggccggcgc cgggagcgat cgctcgctgc
    12721 ggcgggccct gtccgtcatc gccctcggcg tgcgcgccga gccgcggctg ttcgccatcg
    12781 ccgtcacggg aagcgtgctg tatggcgtgc tcaccgccgg catggcgcag gccgtcggct
    12841 gggtgacagc gcatcagatc acgtcggcga tcgacgaggg cgggctcagc cgcgggcggg
    12901 cgctgatcat cggatccgtg atcggcggcg tggtcctcct caccacggtc ggggtgctgc
    12961 tgcggcggat cgggggcggc ttcacggtgt tcaacgtcgg cgccgactac cgccgcaaag
    13021 tcacccgcca atatctgcgc ctgccactgg cctggcacca ccgtcacccg tcgggccagt
    13081 tgctgtccaa cgcccatgcc gacgtcgagg cgacgtggaa tatcttcatg ccgctgccga
    13141 tgtcgctcgg cgtgctcgtc atgctgctcg ccggggccgt ccagatggtg ctgatcgatc
    13201 cggtgctggc gggtgtcggg ctggtcatct tcccgctgct cttcgcggcc aatgccgctt
    13261 tccagcggcg gatgagcgag cgggtcacgc gcgcccagca gttgcgcgcc gaggtcgcgg
    13321 aggtggctca cgaatccttc gagggtgcgc tgctggtcaa ggtgatgggg cgcgagcaac
    13381 gggagacctc ccggttcgcc gaggtcagcg accggctgcg cacggccgcg atccgggccg
    13441 gccgcacacg gggcactttc gacccggtga tcgaggcgat cccgacgttc ggcactctcg
    13501 ccgtcctggg cgtcggggtg gggcgcgtcg cgagcggagc cgtgacgacg gccgagctgg
    13561 tgcaactggc ttatctgatc tccgtcctcg ccttccccgt gcgcgcgttc ggctgggtgc
    13621 tgggcgagat cccgcgcacc ctcgtcgggt gggagcgggt caacgccgtc ctgcaggcgc
    13681 gcggctcgat gagtcacggt gccgcggact tgggccaggg gagcccgggc gcacggctga
    13741 ccatggacgg cgtcacctac tcctacgagg ccgcctcgga tgccgccctg accgggtccg
    13801 gtgctaccgc tgggtccgct gatctccctg ccagtggagc cctcggcgcg ccgatcgcct
    13861 tgcgcgacat cactcttgac gtggcaccgg gcgagacgat cgccatcgtc gggcccaccg
    13921 gggcgggcaa gtccacgctc gcggggctga tcgtgcgctt gatcgatccg accagtggcc
    13981 gcgtgtccat cgacggggtc gacgtgcgcg acctcgctcc cggtgcgctg gccgccgatg
    14041 cggcgctggt cccgcagagc accttcatct tcgacgacac cgtccggggc aatgtcaccc
    14101 tcggtgcgcc ggtgcccgac gcgcaggtct gggcggcgct gcgcacggct catgccgatg
    14161 gcttcatcgc ccggctgccc gacgggatcg acaccaaggt cggcgaacgc ggcgccacgc
    14221 tgagtggcgg ccagcggcaa cggatcgcgt tggcgcgggc actcgttcgc cacccgcgtc
    14281 tcttggtgct cgacgacgcg acgagcgcgg tggacccgac ggtggagcaa gcgatcctgg
    14341 ctgccttggg ggccggcacc ggtgccatga ccgtcgtcgt catcgcctac cggatgtcga
    14401 cgatcgcgct cgccgacgag gtggccttcc tcgacgaggg ccgtcttgtc gaccggggca
    14461 cgcacgccga actgatgtcg cgctgccagg gctatgccga cctggtgacg gcgtatgccc
    14521 gagaagcggc cgagcgggcg gccgtcgccg ccgacgagga accgtcggat cccgtggagg
    14581 tgacgtcgtg agcaccgccc tccccgccaa ctccgccatg cagaccaaac ccgccggggg
    14641 agcctgggca acgctgaagc gcggtgtctc gctctcgccc gagctgacca agggcatcgg
    14701 tgtcaccctg ctgctggcga ttgtgacgac cgccggacga gtcgtcgtgc cggtcgtcgt
    14761 ccagcgcgtc accgacgacg gcctgctcgc cgccgggggt cccgacgccg ggatcgtcta
    14821 tcgctacctc gcgatcgccg ccgtcgtggt ggtcatcacg gcgatctgct cgtatgccgt
    14881 caacgtgcgc ctgttcacgg cgtccgaggg cgggctggcg acgttgcggg tcaaggcgtt
    14941 ccggcacatc cacgatctgt cggtgctgac gcagaacgcc gagcgccgcg gatcgatggt
    15001 ggcgcgggtg acgaccgatg tcgacacgat cagccagttc gtccagttcg gcgggatcgt
    15061 cctgatcgtc agcttgggcc aaatcacgct ggcgacggtg ttgatggtga tctacagccc
    15121 ggtgctggcg gccgtgacct ggggctgctt catccccctc gtcatcgccg tccggcgcat
    15181 gcagaagtcg ctcggcgcgg catacggcga tgtccgcgag cgtgtcggcg acctgctcgc
    15241 cgcggtctcc gaatccgtcg tgggtgctgc gacgatccgc agcttcggcg tcgcggaccg
    15301 caccgccgag cggctcgacg cggcgatcga aggccaccgc aaggccgcga cgaccgcgca
    15361 catccgctcg acgatcgctt tctccgcggg ccagttcgtc ggcggtatca ccctggcggt
    15421 cgtcctcgtc gtcggcacct ggctggccgc gcgcggggac atgaccctcg gtgagctgct
    15481 ggcctatctc ttcctcgtcg gcctcttcac ccagccggtc cagcaggcca cggagatcct
    15541 caacgagttg cagaacgccg tcgcgtgctg gcgccgcgtc atccaggtca tggacacccc
    15601 cgccgatgtg gctgaccccg ggactgacgg gatcgacctg ccgcgggggc cgatcaccgt
    15661 cgacttcgac gatgtcggct tcgcctatcc gggcggccct ccggtgctcc acgacatcac
    15721 cgagcacgtc gcgccgcgca gccggatcgc cattgtggga gagaccggtt cgggcaagtc
    15781 gacgttcgcc cgcctgctga cgcggctgat ggacccgtcg tcggggatcg tgcgcctcga
    15841 cggcgtcgac atccgcgacg tgcgtttcgg ctcgctccgc tcccgggtcg tcatggtgcc
    15901 gcaagagggc ttcctcttcg acgacaccgt gctcgagaac atccgcttcg gcggcgagca
    15961 ctcgcgcgcc gacgtcgagc tcgccgtgac cgagctcggg ctggatgcct ggctgtccgg
    16021 cctgcccgcc ggcctcgaca cccaggccgg ccagcgcggc gagtcgctca gcgcgggcga
    16081 gcggcagctg gtcgcgctgg cccgggccta cctggccgac ccggacctcc tggtgctcga
    16141 cgaggccacc tccgcggtcg acccggccac cgaggtccgt atccagcgcg ccctggactc
    16201 gctcgcccgg ggccgcacct cgatcgccat cgcccaccgc atctccaccg ctcaggccgc
    16261 cgacgaggtg ctcgtcatgg acgccggccg agtcgtccag cgcggcccgc acgcccgcct
    16321 ggtcaccgag gacggaccgt atgccgcgct ctacgcctcg tgggtcgcca accagggcgc
    16381 tcactgaggt gccggcctcg cgttccacgc tgctccgcac ttcccgggcc aggcgccatg
    16441 gacgccgagc tcgagcggcg ccggccagtt ggcacaatga cccggtgagt ctcgaccccg
    16501 ccatcgccgc ccgcctcaag cgggacgccc aggggctggt cgccgcgatc gcccaacagc
    16561 acgacacccg tgaggtgctc atgctcggct ggatggacga cgaggccctg caccggaccc
    16621 tcacctccgg ccgggtgacg ttctggtcgc gcagccgcgg ggagtactgg cgcaagggcg
    16681 acacgagcgg gcacgcccag atcgtcaaga gcgtcgccct cgactgcgac ggcgacgccc
    16741 tcctcgtcac ggtcgaccag atcggcgccg cctgccacac cggcgatcgc acctgcttcg
    16801 acgcgggcga cctcggcgcc accatctcgc ccgatgcgcc cgcgggaggc gaggcatgag
    16861 cgcggggccg gccacactgc catacggcag cacgtggccc gctctcgaga ccttcaccgt
    16921 cctggcccaa gaccggcgcg tcgtgccggt cgtccgccgc ctgctcgccg acggcgaaac
    16981 ccccctcgcc ctctatcgca agctggcaca aggcaaaccg ggcaccttcc tgctcgagtc
    17041 ggccgaacac gggggcgtct ggagccgcta ctcgatcatc ggcgccgcga gccgcgccac
    17101 cctgaccgag cgggacggcg aggccgtctg gatcggcgaa ccgcccgtcg gggtgcccgc
    17161 cggcggcagc cccgtcgatg ccgtccgcga caccgtcgcc gccctcgcca cggaggccct
    17221 cgccggtctg ccgccgctga ccggcgggct cgtcggcgcc atctcctacg acgccgtccg
    17281 gcgctgggaa cgcctgccgg agaaggcgat cggcgacctc gacctgcctg aggtcgcgat
    17341 ggtcctggcc accgacgtcg ccgtcctcga ccacagcgac ggctccgtgc tcctcatcgc
    17401 caacgccgtc aactacgacg acaccgacga gcgtgtcgaa tgggcctgga ccgacgccgt
    17461 cgaacgcctc gacgccatga ccgctgccct cgcgacgccg accgacagca ccgtggccgt
    17521 cctcgacgcc gcccgcgccg acaccgctct cgagaacgtc cggtccaatc tcacccagga
    17581 gcacttcgag gacctcgtcg agcagtgcaa ggaggacatc cgcgcgggcg aggccttcca
    17641 gatcgtcctg tcacagcgat tcgacatcga ctgtcccgca gacggtctcg acgtctatcg
    17701 ggtcctccgc tccagcaacc cgagccccta tatgtacttc ttccggctcg accacccggc
    17761 cagcggatca ttcgacatcg tcggctcatc gcccgaagtg cacctcaagg tcaccggccg
    17821 cgaggtgatc ctgcacccca tcgccggcac ccgaccccgc ggcaagaacc ccgagcacga
    17881 cctgctgctc gaagaggaac tcctcgccga ccccaaggaa cgcgccgagc acgtcatgct
    17941 cgtcgacctc ggccgcaacg acctgcagcg cgtctgctac gccggcaccg tcgacgtcgt
    18001 cgagtttctc aacatccgcc attacagcca cgtcatgcat atcgagtcga cgatcgtcgg
    18061 ccaattggcc agggaggcga cggcatacga cgccctggcc gcgaccttcc ccgccggcac
    18121 cctctccggc gcgcccaagc cgcgggcgat ggcgctcatc gaggaatacg agccgacgcg
    18181 gcgcgggatc tacgggggag tcgtcggcta cttcgacttc cacggcgaca tggacgtcgc
    18241 catcgcgatc cggaccgccg tgatcacgca gggcaaggcc ttcgtccagg ccggggcggg
    18301 catcgtcgcc gacagcgtgc ccctgtccga atacgaagag acccgcaaca aggccgccgc
    18361 ggtgctgcgc gcggtcgccg cggccggcac cctgggaccg ccgcgatgag ccgtatgcgg
    18421 tggaccggcc cccgcgtcgt cgtcccggtg gcgctgctcg cggccggcct cgcggcgatc
    18481 tcgggacgac gggtctggct gacgggccgg gtcgaggatg ccgtcgtcgg gtcgagcacc
    18541 gtcaccggca ccggcaccca ggtggtgccc ggcctcgtcg ccctggcgct cgtgctcgcg
    18601 gccgcggtcc tggcggcagc gacggccggt gccgtcatgc gccggctggc cctcgtgctc
    18661 ggcgccgtcg ccggaatcgc cctggcggtg ctggcggtgc gggcctcgtc gtccggcgcg
    18721 agcctgttgg gcggcctcgc cgccgaggcc tccagccgca ccgggaccat ccccgtcacc
    18781 ggagtgtccg tcggcagctg gccgtggctg gtcgccgcgg ccgcgacggc cggcgccaca
    18841 gcctgcctgg ccggcctcgc cggcgcgtca cagtggcgcg gcctgtcgca acggtatgac
    18901 gcccccgccg ccgagcccgc cggcgccggg ccgcgcggcc agcggaccac gagcgcctgg
    18961 aacgacctcg acgacggcgt cgaccccacc caagccccag gcacaccccc tggcgatccc
    19021 ctgtagacgc ccgtagacgc cagagcgggt gccgagtcgg gggggagggc cgggtgcgct
    19081 gcgcctcagg gcggtgacta gtcgggggcg ggccgggtgc gctgcgcctt gacctgggcg
    19141 cgccggcgct tggccgcgat ccggcgctcc cgcgaccccc gcgtcggcgt ggtggcccgg
    19201 cgcgcaggcg gtggcggcgc cagggcctcc cgcaagagcg ccgcgagccg ctcgcgggcg
    19261 gcgacccggt tgcggcgctg ggcgcggtgc tccgaggccg ccaccgtcac cacatccccg
    19321 agggcggcca cggcgcgtga ccgttgggtc tcggtcagcc acgaggcgtc ggccaggctc
    19381 agttcgacct cgacccggct gtcggtcgtg ttgacgccct ggccgccggg acccctcgag
    19441 cgcgagaacc gttcgcgcag gagctcaccg ggcaccacga gcccggccgg caggcccggc
    19501 cccggaggca cgacaagatc cacatcccca ggttcccacg agaccctccg tgcgacccgg
    19561 cgtggtcgaa tcgtcacacc catgccgtcc caggcgacgc cttctgccac aatcgcttcc
    19621 tgacggaacg gcgccggcca tccaccgcac gaaaggcagg acatgagcac ccacgaggac
    19681 gagcacgaca gccacggcaa cagcacggcc gcctgggtga gcaccatgat catgctcgtg
    19741 gccgccgcgg tcatctgttg ggcgatcatc caggccaaca ccagcctggc catcgccggc
    19801 ggggtcgcct tcctcatcgg tgccctgctg tggccgatcc tggccaagat gggttacggc
    19861 gagaagaacc cggtccgctc gccctacgcc tgaccgaccg cggcccaggc atccgggatc
    19921 cggcctcgaa tcggcagtcc ccgtcgcacc ggttacggtg atcggcatgc ccgccaacgc
    19981 caccgtgctc gattcgatca tcgcgggtgt ccgtgacgac gtggcgcgcc gcgaggaccg
    20041 tatgccgctg cgcgaggtcg tcgcggccgc cgccgccgcg ccacccgccc gaccggtcct
    20101 gccccggctg accaggtcga ccctcggcgt gatcgcggag gtcaagcggt cctcaccgag
    20161 caagggcgca ctcgccgaga tcacggaccc ggggagtctc gccgcggcat accgagaagg
    20221 cggcgcagcc gcgatctccg tcctgaccga gcagcgtcgc ttcggcggca gcctggccga
    20281 cctcgacgcg gtccgcgcgg cggtcgaggt gcccgtgctg cgcaaggact tcatggtctg
    20341 cgactaccag ttccacgagg cgcgcgccca cggcgccgat ctcgtgctgc tgatcgtcgc
    20401 cgccctgacc gacgacgaac tccgccgctt ccacgacctg acccacgaac tcggcatgac
    20461 ggctctcgtc gaagtccacg acgaggccga gatcgaccgc gccctcgccg tcgcgccgcg
    20521 gctgctcggc gtcaacgcgc gcaacctgaa gaccctcgac atcgacgccg acgccttcgc
    20581 gcgcctggtc acccaggtcc ccgacgacgt cgtcgccgtc gccgagagcg gcatcgccgg
    20641 ccccgccgac gcgcagcgct acgcccgcga aggggcgcgc gccgtcctcg tcggcgaagc
    20701 cctcgtcaag gacggcgacc cgcgcgccgc cgtcgccgct atggccgaaa tccccacggg
    20761 agcaacccat gtctgagcaa catacagctg agcaacccaa gtctgagcac ctaatgtccg
    20821 acgccccagg ccgcttcggc cgcttcggcg gacgctatgt gcccgaggcg ctcatccccg
    20881 cgctcgaaca gctcgacgag gtccggcaga aggccatggt cgacccggac ttccaggccg
    20941 aactcgacca cctgcacaag acctacaccg gccggccgag catcatcacc gaggtgcctc
    21001 ggttcgccgc tcacgccggg ggggcccggg tcatcctcaa acgcgaggac ctcaaccaca
    21061 ccggctccca caagatcaac aacgtcctcg gccaggccct gctgaccagg cggatgggca
    21121 agcgccgcat catcgccgag accggcgcgg gccagcacgg cgtcgccacc gccaccgccg
    21181 ccgccctcat gggcctggac tgcaccgtct acatgggcaa ggtcgacacc gagcgccaag
    21241 ctctcaatgt cgcgcgcatg aagatcctcg gcgccgaggt gatccccgtc gagcacggca
    21301 gcgccaccct caaggacgcc atcaacgagg ccctgcgcga ctgggtcacc aacgtccacg
    21361 acacccacta ctgcatcggc accgtcaccg gaccgcaccc cttccccgag atggtccgcg
    21421 acttccacaa gatcatcggc atcgaggcgc gccaacaggt cctcgagctg accgggcggc
    21481 tgcccgacgc cgtcatcgcg tgcgtcggcg gtgggtccaa cgccatgggc atcttccacc
    21541 ggttcgtcga ggacgagggc gtgcggctga tcggcgtcga ggccggggga gagggcgtcg
    21601 agtccggacg gcatgccgcc cgtttcgcct cggccgagcc gggcgtcctg cacggcgcca
    21661 tgtcctatct gatgcaggac gacgacggtc agaccctcga atcccactct gtcagtgccg
    21721 gtttggacta cccgagcgtc gggcccgagc actcgcacct gaaggacatc ggccgcgccg
    21781 aatatcgcta cgccaccgat gaggagacca tggaggcctt ccggctgctg tgccgcaccg
    21841 agggcatcat ccccgcgctt gagtcgtcgc acgcgctcgc cggcgcgctc gacgtgggcc
    21901 gcgagctggg ccccgatggc ctgatcctcg tcaatctctc cggccgcggc gacaaggaca
    21961 tgcacaccgc cgctgcgtgg ttcggtctca tcgacgccca aggggagggc acggtatgac
    22021 gcccgcgacc gccgtcgggt ccgctgcggt tttcgagacc tgccgccagg aggggagggc
    22081 cgccctggtc ggctatctcc cggtcggctt cccggatgtg gcgacctcca tccgcgccat
    22141 ggtcacgctc gtcgagaacg gctgcgacat cgtcgagatc ggcatcccct acagcgaccc
    22201 ggtcatggac gggccggcca tccagcgggc cgcctcggcg gcgctcgccg gcggcacccg
    22261 cgtggaggac gtcttcgccg ccgcgcgggc cgtgcgcgac gcgggagcgg tgcccctggt
    22321 gatgacgtac tggaacctcg tgctacgccg cggcgtcgac cgcttcgccg acgacctcgc
    22381 cgccgccggc ggcggcgggt tgatcacgcc cgacctcatc cccgacgagg ccggcgactg
    22441 gatcgccgcg agcgaccgga cgggcctgga ccgggtcttc ctcgtcgcgc cctcgtctac
    22501 cccagagcgg ctcgccaccg tcaccggtgc ctgccgcggg ttcgtgtatg ccgcctccac
    22561 catgggcgtc accggcgtcc ggtcgtcggt gggcaccgcg gccgatgaac tcgtcgagcg
    22621 cacccgcgcc gcgaccgacc tgcccgtgtg cgtgggattg ggggtcagca ccggggaaca
    22681 agcggcgcag gtcggttcgt tcgccgatgg ggtcatcgtt ggctccgcgc tggtgaccac
    22741 cttgctcgac gtcgagcccg aggccggcct ggcgcgtctg gcagaggtga cccgcgaact
    22801 ggcggccggc gtgagggaga gcaagcgctc atgagcaggc tcagcaactc gacgcgaagc
    22861 gctggaaaat gtggtcgcgg aggccgtcaa ataggtccct gtcggccgtc ccggttagcc
    22921 tgaccaccgc ataccgcacc acccaccgaa ggaagccact cgcagatgtc gaagaagccc
    22981 acggcgcagc gcgggtcccg cgccggcgcg accccgcagg acagccggca ggcgaagatc
    23041 caggccgccc agaaggcgag cgggagcagc gccaacaaga tcaccatcgc cggcatcgtc
    23101 gcgatcctcg cgatcatcgc cgtcgtcggg ggcgtgatct ggaacgagaa gaacaacgag
    23161 aaggaggccg ggtccggcaa caccgcgccg ccggccggcg cgacggtcga tgccggctac
    23221 cgctccttcg ccgacgtgac gctcgcgtcc ggcgcgccgg tggtcaccgt cttcgaggac
    23281 ttccagtgcc ccgcgtgcaa gcagtacgaa gctgcgatgg gcagcacgct ggcccagttg
    23341 gccaaggacg gcaagatcga cctgcgctac cacatcatga acttcctcga cgacaagacc
    23401 ggggccaagc agtccacgcc ggtcgccaat ggcgccttct gcgcggctga ccagggcaag
    23461 ttccaggagt tccacgacgc cgcctacacc gcgcagcacc ccgaggggga ctcggtcacc
    23521 acggcagatg tctcgaagtt cgccacgacc gccggtgtcg gcgatatgga cgcctggacc
    23581 aagtgcgtcg atgccggcaa atacaccagc tacatcaacg cctccaacga gtccgccggc
    23641 accgccggtg tcacgggcac cccgacgctg aagatcggcg acaagctcta ctccggcgag
    23701 acgctgggcg aggtggccac cgccgacggc ctgaccaagg tcgtcgaggc cgccaccaag
    23761 tagcccgggc caggcaggcc gggccaggag cccgtcgggt cacatcgtgg cctgacgagg
    23821 gagggaactg ggtatggcag tgacaccgcc accaccgcag gaccggtcgc cggcgggccg
    23881 cggtgccgag ccgcggccgc cggccggcgc cagccggggc gccgtcgccg ccatcgtggc
    23941 gctggtgacc gtcgtcgccg tcgccgcggg tgtgatctgg tcccagcgat cgggccagga
    24001 ccaggctggc ccgggcgcgt cggtgccggc gagtggcacg gcgagccgga ccgaggagcc
    24061 cgtcccgacg gcgagcgcgg ccgaggcgac ggcgccgaca gccggcaaca cgacgagcac
    24121 ggcgtccgcg tccgcctccg gtccgcagtc gacacccgag ggcgccggcg tggacgaggg
    24181 ctaccgctcg ttcgcggggg tggatctgcg gcccggcgct cccgtggtga ccgtgttcga
    24241 ggacttccag tgcccggcgt gcgcccagtt ggagggagcg atcggggcca ccctgaacga
    24301 gttggccgcg gacggcaaga tccagctcaa ctaccggatc atgaacttcc tcgacgagat
    24361 gaccggcggc acgcagtcga cgcccgtcgc caatggcgcc ttctgtgcgg cggaccaagg
    24421 caaatggcag gaattccacg acgccgcctt cgccgagcag taccccgaag gcacgtcggt
    24481 gagcgtggag cagattgccg ctttcgccag gactgcacag gtcccagacc tcgacgcgtg
    24541 gaccgcgtgc accgagtcgg ccccctacag cacgttcgtc gccgactcca acgccgcggc
    24601 gaccaccgac ggcgtcaccg gcacccccac ggtgtggatc gacgggaagc cggtggcttt
    24661 gcgggacatc atcacgcccg ccgggctgag ggcggcgatc gtggatgcga ccgagtaggc
    24721 ggccgtgatc acctacatcc cgagcccgac ggtgggtgcg ctacacctcg gcccgctggc
    24781 gatccgcggc tacgccctgt gcatcctcac cggcatcgtc gtcgccgtgt ggctcgccgg
    24841 gcgccgcctc gagcaccgcg gcggcaaacg caccgatgcc ctcgacatcg ccacctgggc
    24901 ggtgcccttc ggcatcgtcg gcgcccgcat ctatcacgtc ctcagctcac cggaggccta
    24961 cttcggcgcc gacggccgtc tcatcgacgc cctgaagatc tggcagggcg gactcagcgt
    25021 gtggggggcc atcgcgctcg gggcgctggg ggcgtggatc ggctgccgcc gggccggcgt
    25081 gggtttcctc gacttcgccg attccgccgc cccgggcatc gccatcgccc aggcgctcgg
    25141 ccgctggggc aactggttca acaacgaggt ctacggcgag cgcaccgatg tgccctgggc
    25201 ggtgcagatc caccagtggg ataccgccac cgggtccgcg gtcaaggacg caagcggcga
    25261 cccggtcgtg ctcggcacct tccaccccac cttcctgtac gaggcgatct tctgcctgct
    25321 gctggcgatc gtcctgctga tgctggaccg gcgtcgcgcc ttcgccccgg gtcagctgtt
    25381 cgcgctctat gtcgccggct acccaacctt ccggatcgtc ctggagaaga tgcgcaccga
    25441 cgaggcgacg atcatcctcg ggcagcggat caatgtctgg acctcgatcg ccgtctttct
    25501 cctgggcgtg gggctctacg cgtggacggg gcgccgctcc cggcgcagca cgcccgtctc
    25561 acagggtgag aaatccctga ccacatcgta ggacggcctg atgatagggt gatctcgacg
    25621 tggccgttgc cgtccacgca ggttccctca caagtttgga aaaggacggt gcccgatggc
    25681 ctctgccctc ttctcggccc agcccgccga ccacgggctg tatcgccgcg agttcgagca
    25741 tgacgcgtgc ggcgtggcca tggtggcaac gctgcgcggg catgccggcc acgacatcgt
    25801 cgtgcaggcc ctgacggcac tgaccaacct cgaccaccgc ggcgccaccg gggccgaccc
    25861 gctcgtcggc gacggcgccg gcatcctgac ccaggtgccc gacgccttcc tgcgcgaggt
    25921 cgccggattc accctgccgc cgcgtgggtc gtatgccgtg ggcgccgcct tcctgcccgc
    25981 cgaccctgag gcccgcagcg aggccgtcca ccgcatcgag gacctcgccc acgaggaggg
    26041 cctggaagtc atcggctggc gcgatgttcc cgtcactccc gacctggtcg gcaccgccgc
    26101 ccgcgactgt atgccgcact tcgcccagct cttcctggcg gccagcgacg gggcggtgcg
    26161 cggcatacac ctggaccggc gtgcctactg cctgcgcaag cgcgccgaac gggaggccga
    26221 cgtctacttc gcctcgttgt ccgcgcggac cctggtctac aagggcatgc tgaccaccgg
    26281 tcagctcgag cccttcttcc ccgacctgtc cgatgagcgc ttcgtcaccg agctcgcgct
    26341 ggtgcacagc cgcttctcga ccaacacctt cccgtcgtgg ccgctggccc acccctaccg
    26401 gctgatcgcc cacaacggcg agatcaacac cgtcaagggc aaccgcaact ggatg
//
LOCUS       DAOPJG010000018        24800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2116_length_24800_cov_7.570216, whole genome shotgun sequence.
ACCESSION   DAOPJG010000018 DAOPJG010000000
VERSION     DAOPJG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 24800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24800
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2116_length_24800_cov_7.570216"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..2345)
                     /gene="helR"
                     /locus_tag="PKU75_03350"
     CDS             complement(210..2345)
                     /gene="helR"
                     /locus_tag="PKU75_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor ATPase HelR"
                     /protein_id="HPF79941.1"
                     /translation="MNPLSTNVFDLPDRLAAKADPALIGSDGHHLAAVAGCLAQQRAD
                     LAARLEETRTSPVRMGQEALDRDLQTHRLSARLALLQRFGVDICLGRIDRPDGSVYVG
                     RIGLIDDAGRQLLVDWRSPAAKPFFAATLANPMGLTSRRRYRWTGERITDYWDETFTT
                     DSGATSAALDDQSAFIASLGASRSPRMRDVLGTIAADQDAVVRAGSQGPLVVDGGPGT
                     GKTVVALHRAAYLLYADPRLDPRRGGVLVVGPHQPYLAYVEDVLPSLGEEGVRLCTLR
                     DLVAEGAAARPEGDPAVARLKSARRMVDAIEVAVRFYEDLPAAAMTIDCDGTPVRINP
                     QDWAEAADAPEDGTAHNDARAEIWEALLDILVDRFDAGIPLTQRRQALQRNEELTEAM
                     NHAWPLIDAADLVGDLWSVPAYLRHCGPWLSPEEVLMLQRADASEWTVADLPLIDAAR
                     HRLGDPHASRRRQALAAATAARREEMAKVVDHLIAADDSTLQVMSMLRGADLQHVLVD
                     DALPPAADADALGGPFAHVVVDEAQELTDAEWQMVLRRCPSRSLTIVGDRAQARHGFT
                     ESWMDRLARVGLPTARLATLGVNYRTPVEVMTEAEPVIRSVLPDANVPLSIRSTGIPV
                     RHGSRRELPAILDEWVATHDDGIACVIGDSGYAASPRVRSLSPELAKGLEFDLVVLVD
                     PDGFGEGIEGAVDRYVAMTRATGRLVVLT"
     gene            complement(2589..3620)
                     /locus_tag="PKU75_03355"
     CDS             complement(2589..3620)
                     /locus_tag="PKU75_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HPF79942.1"
                     /translation="MTTKMQAAVATRFRSPLAVTTVPVPAPGPGQVLVRVHACGVCHT
                     DLHTVNGDWPVKPSLPRVPGHEAAGEVVEVGDLVREVAVGDRVGVPWLHSACGRCEQC
                     LTGWETLCDKQTRTGYDVDGGFAEYVLADAAYVAHLPAAMDYLHAAPLMCAGLTVYKG
                     MRMVDAAPGDWVVVSGIGGLGHLAVQYARAMGYQVVAVDIDDSKLDLAARLGASMTVN
                     AKTTDPVSYLQRQLGGVHGALVTAVSPAAFSQAVGMMRPGGTVVLNGVPPGDFALNIY
                     DIVMRAITMRGSIVGTRADLARALELAVQARIETVIHPAHLEDVNEVLDKMQKWPIPG
                     RTVLEFGPR"
     gene            complement(3617..5680)
                     /gene="pta"
                     /locus_tag="PKU75_03360"
     CDS             complement(3617..5680)
                     /gene="pta"
                     /locus_tag="PKU75_03360"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPF79943.1"
                     /translation="MTGAPAHPAIYLCPTEHCPHLLQFAVGLVPEMQRRYGSVGVFRP
                     IVANPATDPVLPTLLRQVANVADLPHWGVTADDVLHNPTTAMADVIARYAECALRYDA
                     MLVLGSEYDCSLAPIEFSWNTRVSANIDVPMAFVVPSAGDAERVIARVDVAIDEATRN
                     HAQVLAVLVPRSEGQPLDAAELEQLPVPAFVIDEITGADSGLEGALASVAADVVAGAR
                     GLAGAPVGDVVVADMGLRHLLPLIGAGSVVLFAGDRSEIPLGMLVGCASKQFPAPAAA
                     IAAGPWPLALHVTTLWDRLAPSVPLLRTTQPIEEVYLNLDQYKRLPALVNPDQLLEAG
                     RVFARAVDSTALFDRPRPIGQADVVTPLMFEHQLLERARTAGRHIVLPEGEEERILRA
                     AARLLALGTCRLTLLGDPATIRAHAQRLGLDIGGAALIDASTSDLRPRFAERYADLRA
                     HKGVTLDQASERVTDPSYFGTMMVLEGQADGMVSGAVNTTAHTIRPALEVIKTKPGVS
                     VVSSVFLMCLPNEVLVFGDCAVNPNPTPDQVADIAISSAATARQFGIEPRVALLSYST
                     GTSGSGPDVEATAEATRLVRERAPELPVDGPLQYDAAVDPGVAGVKRPDSPVAGRATV
                     LIFPDLRTGNNTYKAVQRSSGALAIGPVLQGLNKPVNDLSRGAHVSDIVNTVAITAIQ
                     AGADR"
     gene            complement(5677..6033)
                     /locus_tag="PKU75_03365"
     CDS             complement(5677..6033)
                     /locus_tag="PKU75_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79944.1"
                     /translation="MPRRVLTKSSIDDFLAAGTTEVLLEPADIVTALAKEYAQQRGVR
                     LVPAANAAGGMPRAQQPAAAPGPAEPPVTPAEPASPADARVDAEQVRKAVIAAIGDEP
                     DGLGAIISRVLRSGPS"
     gene            complement(6043..6669)
                     /locus_tag="PKU75_03370"
     CDS             complement(6043..6669)
                     /locus_tag="PKU75_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79945.1"
                     /translation="MAIDEKFQLRAYVHLDRLQPQFAAFVGSISGGAPPVEGMSALYV
                     EMAPGNWVFRVVDKVLKATDVLPGAQLVERQFGMFEVHSWSQADVLRAGEVVLETLEL
                     PKDARIAPQVASEQVITNVHPNQAQVLNRMNRGTMIVGGQTMLVLECEPAAYINLAAN
                     EAEKAAPVFLNHLTSVGVFGRMWMSGTESDILAAQQAAITAITEVTGS"
     gene            complement(6659..7531)
                     /gene="eutJ"
                     /locus_tag="PKU75_03375"
     CDS             complement(6659..7531)
                     /gene="eutJ"
                     /locus_tag="PKU75_03375"
                     /inference="COORDINATES: protein motif:HMM:NF011660.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HPF79946.1"
                     /translation="MTKATSVEKRMRAAATAVSRPKGIDPGRPIKVGVDLGTAFTVMM
                     VTDDGDKPLAGAMQFADVVRDGIVWDFAGAQKVVAGLKERLELATGRALDAGAVTIPP
                     AVSAADHRAHRYVIEGAGISCSAVIDETTAANAVLGVRDGAVVDIGGGTTGVAIIRGG
                     QVVKNVDEPTGGTHLSLVIAGALKMPVEKAEKIKVDARNHERLLSIVRPTLEKVATIV
                     GRAIAGEHVEHIHLVGGTSDFTGIADIMTEITGVPASVAPEPMLVTPLGVAQWAEPIT
                     GAPRNDREGQWYGY"
     gene            complement(7528..8310)
                     /locus_tag="PKU75_03380"
     CDS             complement(7528..8310)
                     /locus_tag="PKU75_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79947.1"
                     /translation="MIVTEADLRDQLRRPTAGARVTVPAGARLSPSATDFVKQWGLVV
                     SHDGAVTATSGPSSPAPRERDWDTASTFPVHLAGEVPKCVSCGSPVEHKADALTQLNA
                     AHYALKTHPRIRLRGQVDSLHAQVLLIQRLARQHDEPDLAQDLGTIAAYCRELTSAEY
                     NERPVADLHLRDWDAERIHQATHDPRGVLGVEHLTIDQDAPELQHWLNLARADSRQIE
                     ITALEAFPSPHHPYGASICHAFNRLSSTFYFLQLRLFASERA"
     gene            complement(8307..8930)
                     /locus_tag="PKU75_03385"
     CDS             complement(8307..8930)
                     /locus_tag="PKU75_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPF79948.1"
                     /translation="MAKRVISAADVTEARASGTLVVPPDAIITPLARDRARDLGVTIT
                     QGAAGAPAPRGEGDELTAKVRAIVTSLLGDGGGGSPAPTSSRARRPVKLARAAEVKPE
                     RFPYPGPPPDMQVKTVDVVTTQDGSPVAAGYMTMTAGSFPWTLTYDEVQIVLEGELHL
                     GGDAGGKVARPGDVLFVPNGSSITFGTPEWVKFVYVTFPANWEEQIR"
     gene            complement(9017..9292)
                     /locus_tag="PKU75_03390"
     CDS             complement(9017..9292)
                     /locus_tag="PKU75_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HPF79949.1"
                     /translation="MIALGMVETKGLIGAIEAADAMVKAANVQLIGKEQIGAAYVTVM
                     VRGDVGAVKAATDAGAAAAGRVGELISVHVIPRPHGDVEMILPQARA"
     gene            complement(9549..11060)
                     /locus_tag="PKU75_03395"
     CDS             complement(9549..11060)
                     /locus_tag="PKU75_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPF79950.1"
                     /translation="MTTANLDADLRSVQQARDLAVGAREAMRSFQFASQQQVDSICSA
                     MVAAAMREAGRLGQLAHDETGFGYAAHKRIKNEFAAQGVWDSIKDIPTCGVLRRDEDR
                     GIVEIGWPVGVLAALTPSTNPTSTAIYKVLIAVKARNAIIVAPHPSAKECTAEAVRVM
                     VEAGEAAGMPKGLVSCMTELSLQGTNELMNHYATSMIIATGGPGMVKAAHSCGKPALG
                     VGPGNVPVYVDRSADIPLAASMIVESKSFDCSTICATEQCVIADRPIAEQLKREMQSR
                     GAHWLTAEESERLAGMMFRASGLMVADYVGRTPQALGTAAGISVPDSARILVADLGGV
                     GPAYPLSREKLTTVLGFMVEDGWRAGCDRAVQLLTFGGDGHSVVIHARDEEAILAFGI
                     EKPAFRILVNTWGSLGGVGYTTGLRPSMTLAPGGIGGAAVSDNITVEHVLNVKRIAYQ
                     VLEPPPAARAHGGVLHAAAPAVGSYAAGSSSDDSASAALIEKLVRQILLELDK"
     gene            complement(11070..11729)
                     /gene="eutL"
                     /locus_tag="PKU75_03400"
     CDS             complement(11070..11729)
                     /gene="eutL"
                     /locus_tag="PKU75_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046336 - ethanolamine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HPF79951.1"
                     /translation="MGILDPVKPSVLAVRLIPNVDRGFAEKLKLDSDRHRALGLITCN
                     IDDALYVSLDEATKFAEVDVVYAKSFYAGSGYPSGPLSGEIIGMLAGPTPAEVRSGID
                     ACIAYCEESAWFYSANDEGTLNFFPHLISSTGTYLSDAASIPAGQPLAYLIAPPLEAT
                     YALDLAMKAADVTMQVYFEPPSETNFAGGLLTGSQSACRAACAAFQDAVLDVAAAPQK
                     Y"
     gene            complement(11750..12652)
                     /gene="eutC"
                     /locus_tag="PKU75_03405"
     CDS             complement(11750..12652)
                     /gene="eutC"
                     /locus_tag="PKU75_03405"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HPF79952.1"
                     /translation="MSQSLEEIVARVVAELRNQGMNPKPAAAQASSVISVDGGDLVID
                     LADPTQDEARHAMLVDNPKDANGLRNLMATTTARLGVGRAGPRPKTQTMLLFQADHGV
                     TQDAIYGVVSEEVKDAFDLFTVTTQVADRQEYLLRPDLGRLLNDVAKATIAERCVKNP
                     DVQICVVDGLSAAAIDNNLRDILPVIQQGLTSAGLTLGTPFFIENGRVGVMNDVNSVV
                     HAKVVVLLIGERPGLGIADAMSAYMGFDPQPGKSDADRDLICMITTHGGTNPLEAGAY
                     IVEFIKRMIKYAASGIKLREVAGD"
     gene            complement(12658..14025)
                     /locus_tag="PKU75_03410"
     CDS             complement(12658..14025)
                     /locus_tag="PKU75_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HPF79953.1"
                     /translation="MILRTKLFGHTYEFPDIKDLLAKANEEKSGDQQAGIAAASAAER
                     VAARHILAEVPLRVLRENPVVPYDDDEVTRAIDDAVNERIYDEIKGWTVGDFREWILS
                     NKTTGADIRRVSNGLTGEMVAAVTKLMGNMDLVLAAKKIPVVTHCGNTMGLKGTLASR
                     LQPNHPTDSVDGIRAATYEGLSFGSGDSVIGINPSDDSVSSVSRLLDMTYEIIDTWQI
                     PTQNCVLAHVTTQMECLKRGSPAGLIFQSIAGSQKAMESFGVSVGLLDEAYELAKKHC
                     FTAGPNYMYFETGQGSALSADANFGADQLVLEARNYAIARRWQPYQVNTVVGFIGPEY
                     LYDSVQIIRAGLEDHFMGKLTGISMGVDVCYTNHAKADQNTNDNLAVLLAAAGCNYIM
                     GIPMGDDAMLSYQTTSFHDAPALRQALDLRPLPEFEAWMVDLGLWKDGQLTDIAGDAS
                     FFLQR"
     gene            complement(14025..15542)
                     /locus_tag="PKU75_03415"
     CDS             complement(14025..15542)
                     /locus_tag="PKU75_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HPF79954.1"
                     /translation="MTSRELLSVGIDVGTTTTQLVLSELTVTNQARAGLVPRLDINAR
                     TVLYQSPAHLTPLAGPDEVDVEALVALIRDEYRTAGVAPAQVETGAVIITGETARTRN
                     AEALLDGLGDLAGDFVVTVAGPNLESQIAGRGSGAADWSARHFASVVNVDIGGGSANA
                     ALFRNGSHVASAAAMVGGRQVTIDPQSGVLTHLAPSGRLIVDELGLDLVLGRVADVRA
                     LRSLTDAMAQITVDLVSGVQSPLGARVALSPPLAIVGPATAYFVSGGVGQLLYDGAPA
                     TTLAEITRWGDVGPLFAASLRDNPQWRSLRVEQPQQTLRATVLGAASQQVTLSGSTIW
                     AEDSHLPIRNAPVIEPALQDTAPGLTDPVAIAQALTRGVERWDRGADNQGDFVVALDL
                     PHRLNYEQISALASGTCDFANSHLPQGRPLVIVTEADYAQVLGQTIKQRLPSVPLVVV
                     DQIHLSEGDFIDIGEPMFDGRVVPVSVKTLVFYEPTTNLSRKDRDDVARPEQEVH"
     gene            complement(15580..15870)
                     /locus_tag="PKU75_03420"
     CDS             complement(15580..15870)
                     /locus_tag="PKU75_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HPF79955.1"
                     /translation="MRIARVVGSAVSTIKADQLRGAKLLIVRPASVADELTGEPFVAV
                     DSVGAGEGELVLVSEGSAGRVTDLTADAPVDAVIMAILDSLEVAGQTTFRKG"
     gene            complement(15875..17116)
                     /locus_tag="PKU75_03425"
     CDS             complement(15875..17116)
                     /locus_tag="PKU75_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HPF79956.1"
                     /translation="MSINEVILWILMICMALGAIDRAIGNRFGLGEKFEDGFNAMGPL
                     ALSMVGVYALSPILAKILRPVIGPVYNALGADPAMFAGTLLANDMGGWPLAQAMTDDP
                     QIQKLSGLVLGAMMGATIVFTIPVALGITQKEDRQFLSRGVLAGIVTIPLGVITSGLI
                     AGIGLGTVLRNTVPILIVAVAIALGLYFKPEAMTRGFELFGKGLVFMISIGLGLGVFE
                     TMTGHQILTGEWALLPVAEGFATVALIAMALAGAFPAVHLIVKFGSKPLSAAGRLLGV
                     NDTAAGGFIATLANNIAMFEIMKKMDARGKILNCAFAVSAAFALGDHLGFTAGVMPDM
                     IAAVIVGKLVAGITAVVVAYLLFARAFPAYLAGDEGQLATVGAGPDMTGNPSPAGARA
                     DDVISPHGPAVETPDPGKPEA"
     gene            17331..18557
                     /locus_tag="PKU75_03430"
     CDS             17331..18557
                     /locus_tag="PKU75_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HPF79957.1"
                     /translation="MDDALLPRNTAALRALLDKGVTMPVPLTVEIGDEVDVEHVSGDG
                     VVIHPGTRIRGASTVISAGCELGREGPVTIEDCRLGRGVALKGGYAARATFLDGANLG
                     LGHHVREGCLLEEESGGAHCVGLKQTILMPFVTLGSLINFCDCLMAGGTSRTEHSEVG
                     SSYIHFNFTPGGDKTTPSLFGDVPRGVLLREPPIFLGGQGGAVGPVRTAYGAVVAAGS
                     VLRSDIREERSLTIVGPPPAMTQRDFTGREYRGINRLVAKNLDYLASLDALEAWYAEV
                     RLPFFAAQGALGVLVGEGAREAIAAGRRERITRLLALAGRMDPGQAPQAAFAAHAQAL
                     TEIFGGSYAAVPESLSTHAAATAAAGAPYVEAIRALPDDLVNAGQHWLAGIIEDRWAD
                     VEALLPGVARLRHREG"
     assembly_gap    18681..18780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19094..20425)
                     /locus_tag="PKU75_03435"
     CDS             complement(19094..20425)
                     /locus_tag="PKU75_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79958.1"
                     /translation="MSTYPPEPTPGGDASAYRGTAAPTIPNRLLSVGDLVSGAFRTLT
                     ADPRLFLLVPVAVAAALLLPVLILVGVGVALASTSDGSAAAAVTALLAAVLAVAVAVV
                     LIRISAALVIAAYRVALGERPALRALWNETRGIIWNVLGLYLLIVGAAVVVGVLAILL
                     IGGSVAADEGFGIGVMLLLLFLAYPVMIWLSVKCAFIFQTVAIEKAGPIEAIRRTFVL
                     TKGMWWVTLGRQLMLSLLALPLMFVFSLASAPFSALTRDSGDASSVAGGIVAAIVMGI
                     GAIALYIFQLYSVVYSTLMYIDARRRESLGGQAHAYGSPQQDYVRPAYQPGGYPYGPA
                     ASGSAAGAVAGSQWNSPAYPGPAPVPSSPYPQQPYGQYPQSTTEAPAYPESGGSLWSG
                     SPSSSDPTAPMPQTGSDAGGSDSGGGSWGGDSSGGGDSGGGGGDSGGSSSD"
     gene            complement(20525..21166)
                     /locus_tag="PKU75_03440"
     CDS             complement(20525..21166)
                     /locus_tag="PKU75_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF79959.1"
                     /translation="MPPPPPTEPQTTPPETPDHARVVLLAGPSGAGKSRLAERLRAAY
                     GWPIVRLDDFYKDFDAPGLPKHPALDVADWDHPDSWDGAAACAALEALITTGCSAMPV
                     YDISRSKRVGEGDITARPTDLVLAEGIFAAELIAPLRERGLLHSAWCVRNRPALTFIR
                     RLARDLKERRKPPLILIKRGVALLRAEPDVVARASQKGAQPATAKDVEAALRA"
     gene            complement(21167..22075)
                     /locus_tag="PKU75_03445"
     CDS             complement(21167..22075)
                     /locus_tag="PKU75_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPF79960.1"
                     /translation="MAARLDVRKTYKLYIGGTFPRSESGRSYEVFAAPARAGATPRFL
                     ANAAQGSRKDARDAVVAARAAQPGWAGATAYNRGQVLYRVAEVMEGRRAQFAEEVRVG
                     EGISPSRADSLVSQAIDRWVWYAGWTDKIAQVAGNPNPVAGPFFNLSVPEPCGVVAIL
                     APQESSLLGLVSVIAPAIATGNTAVVVSSKDRPLPAVTLSECLATSDVPGGVVNIITG
                     DAAEIAPWLASHRDVNGLDLTGTAGVEGLTWGELEASAAETLKLIVRPGGAGARAEEP
                     DWAIEPAISRMTTFLETKTVWHPKGR"
     gene            complement(22078..23517)
                     /locus_tag="PKU75_03450"
     CDS             complement(22078..23517)
                     /locus_tag="PKU75_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPF79961.1"
                     /translation="MPTFEYAPAPESRAVVDIASSYGLFINGEWVDGHGTTFKTVNPA
                     SEEVLADIHEGDASDVDLAVKAARTAYSRVWSRMSGADRGKYLFRIARLLQERAREFA
                     VLESLDNGKPIKETRDVDIPLAAAHFFYHAGWADKLGHAGLGPDPQPYGVVGQVIPWN
                     FPLLMLSWKIAPALATGNTVVLKPAETTPLTALLFAEVCQQAELPPGVVNIVTGARET
                     GQAIVDHPDIDKIAFTGSTGVGKMIARSIAGTRKGVTLELGGKGANIVFDDAPIDQTV
                     EGIVRGIFFNQGHVCCAGSRLLVQESIAEEVTRRLQRRLATLRVGDPLDKNTDVGAIN
                     SRAQLDKITQLSEAGEAEGAQRWDNGCPLPDKGFWFRPTVFSGVTQTHRIAQEEIFGP
                     VLSVLTFRTPQEAVAKANNTPYGLSAGIWTDKGSRILWTADQLKAGVVWANTFNQFDP
                     TSPFGGYKESGYGREGGRHGLAGYLKGSN"
     gene            complement(23532..24470)
                     /gene="deoC"
                     /locus_tag="PKU75_03455"
     CDS             complement(23532..24470)
                     /gene="deoC"
                     /locus_tag="PKU75_03455"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPF79962.1"
                     /translation="MTRLTNAGLTTWLHGLPGVDQVGCEARAAGLGTRSIKNDSKRWA
                     IDLAISMIDLTTLEGADTVGRVRGLAAKALTPDPTDLSTPRPAAVCVYGDMVAAAKEA
                     LGDSGVKVAAVATAFPSGRASVPVKLLDTKDAVAAGADEIDMVIDRGAFLSGNYLEVF
                     NQIAKVKAVCGAARLKVIMETGELVTYDNVRRASWLSMLAGGDFIKTSTGKISPAATL
                     PVTLIMLEAVRDWRDVTGEQIGVKPAGGIRTSKDAVKFLVAVNEVAGEDWLDNHYFRF
                     GASSLLNDLLLQRQKLRTGVYSSAVYIADDSPSTAY"
BASE COUNT         3761 a         8112 c         8883 g         3944 t
ORIGIN      
        1 tttcgcgtat tctcgggctg acggtccacc tcgggatccc gaccgcactg ccgatgtggt
       61 tgctgaccgc cgggggcatc ctggggatgc tcggcgtcct tgcggcggcg gggcggaggc
      121 ggcaaccagg cgtcgaggtg ggtggagctg aactcgatgg ccgttgagaa ctgcccgggg
      181 tccactcgac gggggtgggg gcgcgggtct caggtgagga ccacgaggcg accggtggcg
      241 cgggtcatgg cgacatagcg gtcgaccgcg ccctcgatgc cctcgccgaa gccgtccgga
      301 tcgacgagga cgacgaggtc gaactccagt cctttggcca gctccggtga caaggaccgt
      361 acgcgaggcg acgcggcata cccggaatca ccgatgacac aagcgattcc gtcatcgtgg
      421 gtggccaccc actcatcgag gatcgccggc agctcgcggc gcgacccatg gcgcaccggg
      481 atgccggtgc tgcggatcga cagcgggacg ttcgcgtccg gcaggaccga gcggatcacc
      541 ggctcggcct cggtcatcac ctcgaccggg gtgcggtagt tgacgccgag ggttgccagg
      601 cgagccgtcg gcagccccac gcgcgccagc cggtccatcc aggactcggt gaacccgtgc
      661 cgggcctggg cgcgatcgcc gacgatcgtc aggctgcgcg atgggcagcg acgcaacacc
      721 atctgccact cggcgtcggt gagttcctgc gcctcgtcga cgaccacgtg ggcgaagggc
      781 ccgcccagcg cgtcggcgtc ggccgcggga ggcagcgcgt cgtcgacgag cacgtgctgc
      841 aggtccgcgc cgcgcagcat cgacatcacc tgcagggtcg agtcatcggc ggcgatcagg
      901 tgatcgacga ccttcgccat ctcctcccgg cgggccgcgg tggcagcggc gagcgcctgc
      961 cggcggcggg aggcgtgggg gtcgccgagc cggtgccgcg cggcgtcgat gagtggcagg
     1021 tccgcgaccg tccactcgct cgcgtcggcg cgctgcagca taaggacctc ctccggcgac
     1081 agccacggcc cgcaatgacg cagataggcc ggcaccgacc acaggtcccc gacgaggtcg
     1141 gcggcgtcga tcagcggcca ggcgtggttc atcgcctcgg tcaattcctc gttgcgctgc
     1201 aacgcttgtc gccgctgcgt cagcggtatg ccggcatcga accggtcgac gaggatgtcg
     1261 agcagcgcct cccagatctc ggcgcgggcg tcattgtggg cagtcccgtc ctcgggtgcg
     1321 tcggcggctt cggcccagtc ctgtgggttg atccggaccg gcgtcccgtc gcaatcgatc
     1381 gtcatcgccg cggccggcag gtcctcgtag aagcgcaccg cgacctcgat cgcgtcgacc
     1441 atccggcgcg cggacttcag gcgggcgacg gctgggtcgc cctccgggcg cgccgcagcg
     1501 ccttccgcga cgagatcgcg cagggtgcac aggcgcacgc cctcctcgcc gaggctcggc
     1561 agcacgtcct cgacgtaggc gagatagggc tggtgggggc cgaccacgag cacgccgccg
     1621 cgtcgcggat cgagccgggg gtcggcatac agcaggtatg ccgcgcggtg gagggcgacg
     1681 accgtcttgc ccgtccccgg gccgccgtcg acgacgagcg gaccctggga gccggcgcgg
     1741 acgacggcgt cctgatccgc ggcgatggtg ccgagcacgt cccgcatccg gggcgagcgg
     1801 ctcgcgccaa ggctggcgat gaaggccgac tggtcgtcca gcgcggcgga tgtggcgccg
     1861 ctgtcggtgg tgaaggtctc gtcccagtag tcggtgatcc gctcgccggt ccagcggtag
     1921 cggcgccggc tggtcagccc catcgggttg gcgagggtgg cggcgaagaa gggcttcgcc
     1981 gcgggggagc gccagtcgac gagcaattgc cggccggcgt cgtcgatgag gccgatgcgg
     2041 ccgacataga ccgagccgtc ggggcgatcg atccgcccaa gacagatgtc gacgccgaag
     2101 cgctgcagga gtgcgagccg ggcgctgagg cgatgggtct gcaggtcccg gtcgagcgcc
     2161 tcctggccca tgcggaccgg ggaggtgcgg gtctcctcga ggcgcgccgc gaggtcggcg
     2221 cgctgctgcg caaggcagcc ggccacggcg gccaagtggt gtccgtcgct cccgatgagg
     2281 gccgggtcgg ctttggcggc caggcggtcg ggcaggtcga aaacattggt gctcaaggga
     2341 ttcatgggtg tcgtgtccga tccgggccac ctcaggcggc ggtcctgccg gagcattgtg
     2401 cgcctcgccg ggggccttgc cacaagcccc ccggtggggt atagcttggt aggggaggag
     2461 ggacactgtg ccctgaccgg cgtcgccttg ccgggccgcc ctggactagg tcgacgggag
     2521 gcccccggcg cgatggcggg ccgccgaccc cactcgaggg agcagagccg ggcgtgggcg
     2581 ccgcgccctc accgtgggcc gaactccagg accgtgcggc cggggatggg ccacttctgc
     2641 atcttgtcga gcacctcgtt gacgtcttcg aggtgggcgg gatgaatgac ggtctcgatc
     2701 ctggcttgca cggccagttc gagggcgcgg gccaggtcgg cgcgcgtgcc gacgatcgat
     2761 ccgcgcatcg tgatcgcgcg catgacgatg tcatagatgt tgagggcgaa gtcccctgga
     2821 gggacgccgt tgaggaccac ggtcccgccc ggccgcatca tcccgacggc ctggctgaag
     2881 gccgccggcg acacggccgt cacgagggcg ccgtggacgc cgccgagttg ccgctgcaga
     2941 tagctgaccg ggtcggtggt cttggcgttg acggtcatcg acgcgccgag ccgggcggcg
     3001 aggtccagtt tggagtcgtc gatgtcgacg gcgacgacct ggtagcccat cgcgcgggcg
     3061 tactggaccg cgaggtgccc cagcccgccg atgcccgaga ccacgaccca gtcaccgggg
     3121 gccgcgtcga ccatccgcat ccccttgtag acggtcagcc ccgcgcacat caggggggcc
     3181 gcgtgcagat agtccatggc cgccggcagg tgcgcgacat atgccgcgtc agccaggacg
     3241 tattcggcga acccgccgtc gacgtcgtat cccgtgcgag tctgcttgtc gcagagggtt
     3301 tcccaccccg tcaggcactg ctcgcaccgg ccgcaggcgg agtgcagcca cgggacgccc
     3361 acccggtcgc cgacggcgac ctcacgcacc aggtcaccga cctcgaccac ctcgccagcc
     3421 gcctcgtggc ccggcacccg cggcaacgac ggcttgaccg gccagtcgcc gttgacggtg
     3481 tgcaggtccg tgtgacagac cccgcacgcg tgcacccgca ccagcacctg cccaggccct
     3541 ggcgccggga ccggcaccgt cgtgacggcc aagggtgagc ggaaccgcgt tgccaccgca
     3601 gcctgcatct tcgtggtcat cgatcggctc ctgcctggat cgcggtgatc gcgacggtgt
     3661 tgacgatgtc cgagacatgg gcgccgcggg agagatcgtt gacgggcttg ttcagcccct
     3721 gcagcaccgg gccgatggcg agggcgccgg agctgcgctg gaccgccttg taggtgttgt
     3781 tgccggtgcg caggtccggg aagatcagca ccgtggcccg gcccgccacg ggcgagtccg
     3841 gccgtttgac gcccgcgacg ccggggtcga cggccgcgtc atactgcagc gggccgtcca
     3901 ccgggagctc gggggcgcgt tctcggacca ggcgagtggc ctcggcggtg gcctcgacat
     3961 cgggtccgct gccggatgtg ccggtcgaat aggacagcaa cgcgacccgg ggttcgatac
     4021 cgaactgtcg agcagtggcc gcggaactga tggcgatatc ggcgacctga tccggcgtgg
     4081 ggttcgggtt gacggcgcag tcgccgaaga cgagcacctc attgggcagg cacatgagga
     4141 agaccgagga gacgaccgag accccgggct tggtcttgat gacttcgagg gcagggcgga
     4201 tcgtatgggc agtggtgttg actgccccgg acaccatccc gtcggcctgg ccctccagca
     4261 ccatcatggt gccgaagtag ctgggatcgg tcacgcgttc gctcgcctgg tcgagggtga
     4321 cgcccttgtg ggcacgcagg tcggcgtagc gctcggcgaa acgtggacgc aggtcgctgg
     4381 tgctggcgtc gatcagcgcg gcgccgccga tgtcgagccc gaggcgctgc gcgtgcgcac
     4441 ggatcgtcgc cggatcacca agcagcgtca gccggcaggt cccgagggcg agcagccgtg
     4501 ccgccgcgcg caggatgcgc tcctcctccc cttcgggcag gacgatatgg cgaccggccg
     4561 tgcgggcccg ctccaggagc tgatgctcga acatcaacgg ggtgaccaca tcggcctgac
     4621 cgatcggacg gggccggtcg aagagcgcag tgctgtccac cgcgcgcgcg aaaacccggc
     4681 cggcctcgag cagctggtcg gggttgacca gggccggcag gcgcttgtac tggtcgaggt
     4741 tgagatagac ctcttcgatc ggctgcgtcg tgcggagcaa gggcacggat ggcgccagcc
     4801 ggtcccagag cgtggtgacg tgcaaggcca agggccaggg gccggccgcg atagccgcgg
     4861 cgggggcagg gaactgcttg ctggcgcacc cgacaagcat cccgagcggg atctcgctgc
     4921 ggtcgccagc gaagagcacg acggagccgg cgccgatcag cggcagcagg tgccgcagtc
     4981 ccatgtcggc cacgacgaca tcgcccaccg gtgcgccggc cagtccacga gcgcccgcca
     5041 cgacgtcggc ggcgaccgag gcaagtgcgc cctccaggcc gctgtcggcc ccggtgatct
     5101 cgtcgatgac gaacgccggc accggcagct gctccagctc ggcggcgtcg aggggctgcc
     5161 cctcggatcg ggggaccagc accgccagca cctgcgcgtg attgcgcgtc gcctcgtcga
     5221 tcgcgacgtc gacccgggcg atgactcgct cggcgtcgcc ggccgagggc accacgaaag
     5281 ccatggggac gtcgatattc gccgagacgc gggtgttcca cgagaactcg atcggcgcca
     5341 ggctgcagtc gtattcgctg ccgaggacga gcatcgcgtc atagcgaagc gcacactcgg
     5401 cataccgggc gatgacgtcg gccatcgccg tcgtcgggtt gtgaagcaca tcgtccgcgg
     5461 tcacccccca atggggcagg tccgcaacgt tggccacctg tcgcagcagc gtcggcaaca
     5521 ccgggtcggt ggcgggattg gcgacgatcg gccggaagac gcccaccgac ccgtagcgcc
     5581 gctgcatctc gggcaccagc ccgaccgcga actggagcag gtgtgggcag tgctcggtcg
     5641 ggcacagata gattgccgga tgcgcggggg cgccggtcac gaaggtcccg agcggagcac
     5701 gcgcgagatg atcgcgccca gcccgtcggg ttcatctccg atcgccgcga tgacagcctt
     5761 gcgcacctgc tcggcatcca cccgcgcatc tgccggcgag gccggctcag ccggagtgac
     5821 cggaggctcg gcaggaccag gtgcggcggc cggctgctgc gcgcgcggca taccgccggc
     5881 cgcattggcg gcagggacca gccgcacccc ccgctgctgc gcgtattcct tcgcgagggc
     5941 ggtgacgata tcggcaggct ccaggaggac ttctgtggtc ccggcggcga ggaagtcgtc
     6001 gatggaggac ttggtgagga cacggcgcgg cacggtgagc tcctagcttc cggtgacttc
     6061 ggtgatcgcg gtgatggccg cctgctgggc agcgaggatg tcggactcgg tcccggacat
     6121 ccacatgcgg ccgaagaccc cgacggaggt gaggtggttg aggaacacgg gcgcggcttt
     6181 ctcggcctcg ttggcggcga ggttgatata cgccgccggc tcgcactcga ggaccagcat
     6241 ggtctggcca ccgacgatca tcgtcccccg gttcatccgg ttgagcacct gagcctggtt
     6301 ggggtgcaca ttggtgatga cctgctcgga ggccacctgc ggcgcgatcc tggcatcctt
     6361 gggcaactcc agcgtctcga ggacgacctc accggctcgc agcacatcgg cttgcgacca
     6421 cgagtgcacc tcgaacatcc cgaactgccg ctcgaccaac tgcgccccgg gcagcacgtc
     6481 ggtggccttg agcaccttgt cgacgacccg gaacacccag ttgcccggcg ccatctcgac
     6541 atacagcgca ctcatcccct cgacgggtgg cgcgccgccg gagatcgagc cgacgaaggc
     6601 ggcgaactgc ggttgcagcc ggtcgaggtg cacataggct cgtagctgga acttctcgtc
     6661 aatagccata ccactgtccc tctcggtcgt tcctcggtgc gccggtgatc ggctcggccc
     6721 attgcgccac cccgagtggg gtcacgagca tgggctcagg tgccacactg gccggcaccc
     6781 cggtgatctc ggtcatgatg tcggcgatgc cggtgaagtc gctggtgccg ccgacgaggt
     6841 ggatgtgctc gacgtgctcg ccggcgatgg cgcgcccgac gatcgttgcg accttctcca
     6901 gcgtcggccg gacgatcgac aacagccgct cgtgattgcg ggcgtcgacc ttgatcttct
     6961 cggccttctc gacgggcatc ttcagcgcgc cggcgatcac gagggacagg tgcgtccccc
     7021 cggtcggttc gtcgacattc ttgacgacct gcccgcctcg gatgatcgcg accccggtcg
     7081 tgcccccacc gatgtcgacg accgccccat cacgaacccc gaggacggca ttggcggcgg
     7141 tggtctcgtc gatgacggcc gagcaggata tgccggcgcc ctcgatgaca tagcggtgcg
     7201 cgcggtgatc cgccgcggag accgcgggcg ggatcgtcac cgcgccggcg tcgagggccc
     7261 ggcccgtcgc gagctcaagg cgttccttca gccccgcgac gaccttctgg gcgcccgcga
     7321 agtcccaaac gatcccgtcg cggaccacgt cggcgaactg catcgccccg gccagcggct
     7381 tgtcgccgtc gtcggtgacc atcatgacgg tgaacgccgt gcccaggtcg acgccgacct
     7441 tgatcggccg gccggggtcg atccccttgg gccgggagac ggccgtggcg gcggcgcgca
     7501 tccgcttctc gacgctggtg gccttggtca tgcgcgctcg ctcgcgaaca gccgcagctg
     7561 gaggaagtag aaggtgctgg agagccggtt gaacgcgtgg cagatactcg cgccgtatgg
     7621 gtggtggggc gaggggaatg cctcgagcgc ggtgatctcg atctgccgcg agtcggcgcg
     7681 cgccaggttg agccagtgct gcagctcggg ggcgtcctgg tcgatggtca ggtgttcgac
     7741 gccgagaacc ccgcgggggt cgtgggtcgc ctggtggatg cgctcggcgt cccagtcgcg
     7801 caggtggagg tccgcgaccg ggcgctcgtt gtattcggcg gaggtaagtt cccggcagta
     7861 tgccgcgatc gtccccagat cttgcgccag gtccggctca tcgtgctgcc gcgcgaggcg
     7921 ttggatgagc aggacctggg cgtgcaagga gtccacctgc ccgcgcaggc ggatccgagg
     7981 gtgggtcttg agggcatagt gcgcggcgtt gagttgcgtc agcgcgtcgg ctttgtgctc
     8041 gacgggggag ccacaggaga cgcatttcgg gacctccccg gccaaatgaa ccgggaaggt
     8101 ggatgccgtg tcccaatcgc gctccctggg ggccggggac gatgggccgg aggtggccgt
     8161 cacggcgccg tcgtggctga cgaccagacc ccactgcttt acgaaatctg ttgcggaggg
     8221 cgacaatcgg gcaccggccg gcaccgtgac gcgtgcgccg gcggtggggc ggcgcagctg
     8281 gtcacgcagg tcggcctcgg tgacgatcat cggatctgct cctcccaatt cgccgggaag
     8341 gtgacataga cgaatttcac ccattccggc gtgccgaagg tgatcgagga gccgttgggg
     8401 acgaagagca cgtcgcccgg cctggcgacc ttgccgccgg catccccgcc cagatgcagt
     8461 tcgccttcga ggacgatctg gacctcgtca taggtgaggg tccaggggaa gctgcccgcc
     8521 gtcatcgtca tatagcccgc ggcgacaggg gagccgtcct gcgtggtgac gacatcgacg
     8581 gtcttgacct gcatgtccgg cggcggcccc ggatagggga agcgctcggg cttgacctcg
     8641 gcggcgcgcg cgagtttgac cggccgccgg gcccggctcg aggtgggggc ggggctaccg
     8701 cccccaccgt cacccagcaa tgacgtcacg atcgcccgca cctttgccgt cagctcatcg
     8761 ccctcgccgc gcggggcggg tgccccagcg gcgccctggg tgatcgtgac ccccaagtcg
     8821 cgggcccggt ctcgggcaag cggggtgatg atggcgtcgg gcgggacgac cagcgttccg
     8881 gaagcgcgcg cctccgtcac atcggccgcc gaaatgactc tcttggccat ggtgtgctcc
     8941 gtgaactggc gggccggccc aggtgggcct gcccgcagcg attgttaatc gggtgctgtg
     9001 actcgggtgc cgtggatcag gcgcgagcct gcggcaggat catctcgacg tcgccatgcg
     9061 ggcgcgggat gacgtgcacc gagatcagct cgccgacgcg gccggcggct gcggcaccgg
     9121 cgtccgtggc ggccttgacg gcgccgacat cgccgcggac catcacggtc acataggcgg
     9181 cgccgatctg ctccttgccg atcagctgca cgttggcggc cttgaccatc gcatcggcgg
     9241 cctcgatcgc cccgatgaga cctttggtct cgaccatgcc gagcgcgatc atctggacat
     9301 ctgccatggg agtactcctc ttgggttgtg tggacggggt gtcgctgggt tgtgttgtgc
     9361 tgggtggttc tggcgtgctg ggtgtttcgg ctgtgctggg tgtctcgggt gtgctgggtg
     9421 tttcgggtgt gctgggtggt tccggtgtgc tggccgtggc cgtctcgtcc ggcgcgaccg
     9481 cgcgcggtct gcgctggcgg gtggtcttgg ttgtcgtctc gctcatgtgc ctatcccttc
     9541 gggccccctc acttgtctag ttccagcagg atctggcgca ccagcttctc gatcagcgcg
     9601 gcactcgcgc tgtcatcgct gctggagccc gcggcatacg acccgaccgc gggcgcggct
     9661 gcgtgcagca ccccgccgtg cgccctggcc gccgggggcg gctcgagcac ctggtaggcg
     9721 atgcgtttga cgttcaggac gtgttcgacg gtgatgttgt cgctcacggc ggcgccgccg
     9781 atcccgccag gggccaaggt catcgagggg cgcaggccgg tcgtgtagcc gacaccgccg
     9841 agggagcccc aggtgttgac gaggatccgg aaggcaggct tttcgatccc gaacgcgagg
     9901 atggcctcct cgtcccgggc gtggatgacg accgagtgcc cgtccccgcc gaatgtgagc
     9961 agttgcacgg cgcgatcgca tcccgctcgc cagccgtcct cgaccatgaa gccgaggacc
    10021 gtggtcagct tctcccggga gagcgggtat gccggcccca cgccaccgag gtcggcgacc
    10081 aggatccgcg cgctgtccgg caccgagatc ccggccgccg tgcccagcgc ctggggggtg
    10141 cgccccacgt agtcggcgac catcagcccg ctggcgcgga acatcatgcc ggcgagccgc
    10201 tcggactcct ccgcggtgag ccaatgggct ccgcgagatt gcatctcgcg cttgagctgt
    10261 tcggcgatcg gccggtcggc gatcacgcac tgttcggtgg cgcagatcgt cgagcagtcg
    10321 aaggacttcg actccacgat catcgacgcg gcgagcggga tgtccgccga ccgatcgaca
    10381 tacaccggga cattgcctgg gcccacgccc agggccggct tgccgcagct gtgcgcggcc
    10441 ttgaccatgc ccgggccccc cgtggcgatg atcatcgacg tggcgtagtg gttcatcaac
    10501 tcgttggtcc cctgcaggct cagctccgtc atgcagctga ccaagccctt gggcatcccg
    10561 gcagcctcgc cggcttcgac catcacccgc acggcctcgg ccgtgcattc cttggcggac
    10621 gggtgcggcg ccacgatgat cgcattgcgg gccttgacgg cgatgagcac cttgtagatc
    10681 gccgtcgacg tcggattggt cgagggggtc agggcggcga gaacgcccac gggccaaccg
    10741 atctcgacga tcccgcggtc ctcatcgcgt cgcagcaccc cgcacgtggg gatgtccttg
    10801 atcgaatccc agacaccctg ggcagcgaac tcgttcttga tccgcttgtg ggcggcatac
    10861 ccgaagccgg tctcgtcgtg cgcgagttgc ccgagccgcc cggcctcccg catcgccgcg
    10921 gcgaccattg ccgagcagat gctgtcgacc tgctgttgcg aggcgaactg gaaggaccgc
    10981 atcgcctccc gggccccgac ggcgagatcc ctggcctgct ggaccgaccg taggtcagca
    11041 tccagattgg cggtagtcat cgcgtgtcct cagtacttct ggggagcggc ggcgacgtcg
    11101 agcacggcgt cctggaaggc tgcgcacgcg gcccggcagg cactctggct gccggtcagc
    11161 aggccgccgg cgaagttggt ctccgagggc ggttcgaaat agacctgcat cgtgacgtca
    11221 gcggccttca tcgccaggtc gagggcatag gtcgcctcca ggggaggggc gatcagatag
    11281 gccagcggct gcccggccgg gatgctcgcg gcgtcggaca gataggtgcc ggtgctgctg
    11341 atcaagtgcg ggaagaagtt gagcgtgccc tcgtcgttcg ccgagtagaa ccacgccgac
    11401 tcctcgcaat aggcgatgca cgcgtcgatg cccgagcgaa cctcggccgg ggtcggcccg
    11461 gcgagcatgc cgatgatctc gccggacaac gggccggagg gatagccgga gccggcgtag
    11521 aagctcttcg catagaccac gtcgacctcg gcgaacttgg tcgcctcgtc caaggagaca
    11581 tagagcgcgt cgtcgatatt gcaggtgatc aggcctagcg cgcggtggcg gtcggagtcc
    11641 agcttgagct tctcagcgaa gcctctgtcg acattgggga tgagacgaac ggcgagcacg
    11701 gacggcttga ccggatccag gattcccatg atttgacctc tctcggctct cagtcgccgg
    11761 cgacttcgcg cagcttgatc ccgctggcgg cgtacttgat catgcgtttg atgaattcga
    11821 cgatgtaggc gcccgcctcg agcgggttgg tgccgccgtg cgtggtgatc atgcagatca
    11881 ggtcacggtc ggcgtccgac ttgcccggtt gcgggtcgaa gcccatgtag gcgctcatcg
    11941 cgtcggcgat cccgaggccc gggcgttcgc cgatgagcag gacgacgacc ttcgcgtgca
    12001 cgacggaatt gacgtcgttc atgaccccga cccggccgtt ctcgatgaag aacggtgtcc
    12061 ccagggtgag gcccgccgat gtcagcccct gctggatgac cggcaggatg tcgcgcaggt
    12121 tgttgtcgat ggccgcggcg gagaggccgt cgaccacgca gatctggacg tcggggttct
    12181 tcacgcagcg ttcggcgatg gtcgccttcg ccacgtcgtt gagcaggcgc ccgagatcgg
    12241 ggcgcaggag atactcctgg cggtcggcga cctgggtcgt gaccgtgaag aggtcgaagg
    12301 cgtccttgac ctcctccgag acgacgccat agatcgcgtc ctgcgtgacg ccgtgatcgg
    12361 cttggaacaa caacatcgtc tgggtcttcg ggcgcgggcc ggcgcgcccg accccgagcc
    12421 tggcggtcgt ggtcgccatc aggttgcgca gcccgttggc gtccttggga ttgtcgacga
    12481 gcatggcgtg ccgggcctcg tcctgggtcg ggtcggcgag gtcgatgacg agatcgccac
    12541 cgtcgacgga gatgaccgag gatgcttgcg ccgcagccgg tttcgggttc atcccctggt
    12601 tgcgcagctc cgcgacgacg cgggcgacga tctcttcaag gctctggctc atggtgttca
    12661 cctctggagg aagaaggacg cgtcgccggc gatgtctgtg agctgcccgt ctttccacag
    12721 cccgagatcg accatccagg cttcgaactc gggcagtggc cgcaggtcca gagcctgtcg
    12781 cagggcaggc gcatcatgga agctggtggt ctggtaactc agcatcgcgt cgtcacccat
    12841 ggggatgccc atgatgtagt tgcagccggc ggccgcgagg aggactgcga ggttgtcgtt
    12901 ggtgttctgg tcggccttgg cgtggttggt gtagcagacg tcgacgccca tcgagatgcc
    12961 cgtcagcttg cccatgaagt ggtcctccag gccggcgcgg atgatctgca ccgagtcgta
    13021 gaggtattcc ggcccgatga agccgaccac cgtgttcacc tgatagggct gccaccgccg
    13081 ggcgatggcg tagttgcggg cctcgagcac cagctggtcg gcgccgaagt tggcgtccgc
    13141 cgacagggcc gagccttggc cggtctcgaa gtacatatag ttcggccccg cggtgaagca
    13201 gtgcttcttg gccagctcgt aggcctcgtc caggagcccg acggacacgc cgaagctctc
    13261 catcgccttt tgcgatccgg cgatcgactg gaagatcaac ccagccggtg atccccgctt
    13321 gagacattcc atctgggtgg tcacgtgcgc caggacgcag ttttgtgtgg ggatctgcca
    13381 ggtgtcgatg atctcgtagg tcatatccag caaccggctg acggagctga ccgagtcgtc
    13441 cgaggggttg atgccgatga ccgagtcgcc ggagccgaag gagaggccct cgtaggtcgc
    13501 agcccggatg ccgtcgacgg agtccgtcgg gtggttgggc tgcagccggg aggcgagggt
    13561 gcccttgagc cccatcgtgt tgccgcagtg ggtgacaacc gggatcttct tcgcggccag
    13621 gaccaggtcc atattgccca tgagcttggt gaccgccgcc accatctcac cggtcaggcc
    13681 attgctcacg cgtcgtatgt cggcgcctgt ggtcttgttg gacaggatcc actcgcggaa
    13741 gtcgccgaca gtccacccct tgatctcgtc gtaaatccgc tcgttgaccg cgtcgtcgat
    13801 cgcgcgggtc acctcgtcgt cgtcataggg gacgacgggg ttctcgcgga gcacccgcag
    13861 cggcacctcg gccaggatat ggcgggccgc cacgcgttcc gcggcactgg cggcggcgat
    13921 gcccgcctgc tggtcacccg acttctcctc gttggccttg gcgagcaagt ccttgatgtc
    13981 ggggaactcg tatgtgtgcc caaacaactt ggttcgcaaa atcatcagtg cacctcctgc
    14041 tcgggacggg ccacgtcgtc tcgatccttt ctcgacagat tggtggtggg ttcatagaac
    14101 acaagggtct tgaccgacac cgggaccacg cggccgtcga acatgggctc gccgatgtcg
    14161 atgaagtcgc cctcgctgag atggatctgg tccacgacga cgagcgggac gctgggcaac
    14221 cgctgtttga tggtctgtcc caggacctgc gcataatcgg cctccgtgac gatcaccaac
    14281 ggacgccctt gcgggagatg ggaattggcg aagtcgcagg tgccggaggc gagggcggag
    14341 atctgctcgt aattgagccg atgcggcagg tccaaggcca cgacgaagtc gccctggtta
    14401 tccgcgcctc ggtcccaccg ctcgacgcca cgcgtcaagg cctgggcgat ggcgacggga
    14461 tcagtcaggc ccggggcggt gtcctgcagc gcgggctcga tcaccggtgc gttgcggatc
    14521 ggcaggtggc tgtcctcggc ccagatggtc gaaccggaca gggtcacctg ttgggaggcg
    14581 gcaccgagga cggtggcgcg cagggtctgc tggggctgct cgacccgcag cgaacgccac
    14641 tgcggattgt cccgcagcga ggcggcgaac agcggcccga catcgcccca ccgggtgatc
    14701 tcggcgagcg tcgtcgcggg cgccccgtca tacagcagct ggcccacgcc gccggagacg
    14761 aagtaggccg tcgccgggcc gacgatcgcc agaggcggac tgagggccac ccgcgcgccg
    14821 agcggtgact ggacaccgga gaccaggtcg acggtgatct gcgccatggc atcggtcagg
    14881 gaccgcaggg cccgcacgtc ggccaccctc ccgagaacca gatccaagcc cagctcatcg
    14941 acgatgagac ggccggaggg ggcgaggtgg gtcaggaccc cggactgcgg gtcgatcgtc
    15001 acctgtcggc caccgaccat ggccgcggct gacgcgacat gggagccatt gcgaaagagc
    15061 gcggcattgg ccgagccgcc gccgatgtcg acattgacca cgctcgcgaa gtgccgggcc
    15121 gaccagtcgg ccgcgcccga accgcggccg gcgatctgcg attcgagatt gggtccggcg
    15181 accgtgacga cgaaatcgcc ggcgagatcg cccaggccgt cgaggagcgc ctccgcgttg
    15241 cgggtgcgcg cggtctcgcc ggtgatgatg acggcgccgg tctcgacctg cgccggtgcc
    15301 acgccggccg tgcggtattc gtcgcggatc agcgcgacca aggcctcgac atccacctcg
    15361 tcgggcccgg ccaagggggt cagatgcgcg ggcgactgat agagaacggt ccgggcattg
    15421 atgtccagcc ggggcacgag gccggcccgc gcctggttgg tcaccgtgag ttcggagagc
    15481 acgagttgcg ttgtcgtcgt gccgacgtcg atcccgacac tcagcagttc tctgctggtc
    15541 acttcggccc tccttccggg gctgcccgtt gggtgctgtt cagcccttgc ggaaggtggt
    15601 ctgcccggcc acctcgagag aatccaggat cgccatgatg acggcatcga ccggtgcgtc
    15661 ggctgtcagg tcggtgaccc ggccggcgct gccttcgctc acgaggacca gttcaccctc
    15721 accggcgccg acggagtcaa cggcgacgaa cggctcgccg gtgagttcgt cggcgaccga
    15781 tgcgggtcgc acgatcagca gcttggcgcc gcggagctgg tcggccttga tggtggagac
    15841 tgccgatccg acgacacgcg cgatccgcat gccctcaggc ctccggcttg cccgggtcgg
    15901 gggtctcgac ggccggcccg tggggggaga tgacatcgtc ggcgcgggcc ccggcggggg
    15961 agggattgcc cgtcatgtct gggcctgctc cgaccgttgc gagctggccc tcgtcgccgg
    16021 cgagatatgc cgggaaggcg cgggcgaaca gcaggtaggc cacgacgacc gcggtgatgc
    16081 cggccacgag tttgccgacg atgacggcgg cgatcatgtc gggcatgacg ccggcggtga
    16141 agccgaggtg gtctccgagg gcgaaggcgg cgctcaccgc gaacgcgcag ttgagaatct
    16201 tgccccgcgc atccatcttt ttcatgatct cgaacatcgc aatgttgttg gccaaggtag
    16261 caatgaaccc accggcagcg gtgtcattga cgcccaagag ccgccctgcc gcggaaagcg
    16321 gtttggagcc gaacttgacg atcaagtgca ccgccgggaa agcgccggcc aacgccatcg
    16381 cgatcagggc aactgtcgca aaaccttcgg cgacaggcag gagcgcccac tcgcctgtga
    16441 gaatctgatg tcccgtcatg gtctcgaaca cgccgaggcc cagtccgatg gaaatcatga
    16501 acaccaggcc cttgccgaag agttcgaatc ccctcgtcat ggcctccggc ttgaaataaa
    16561 ggccgagggc gatagcgacg gccacgatga ggatggggac ggtgttgcgc aggaccgtgc
    16621 cgagaccgat gccggcgatg aggccggacg tgatgacgcc caaggggatc gtcacgatgc
    16681 cggcgagtac gccgcgggag aggaattgcc gatcctcttt ctgggtgatc cccaaggcga
    16741 ccgggatggt gaacacgatc gtcgcgccca tcatcgcgcc cagcacgagg ccggagagct
    16801 tctggatctg cggatcgtcc gtcatggctt gggcaagtgg ccaaccaccc atgtcgttgg
    16861 cgagcagcgt gccggcgaac atcgcgggat cggctcccag ggcgttgtat accgggccaa
    16921 tcaccggtcg caggattttg gcgaggatcg gcgataaagc atagacgcca accatggaca
    16981 gtgccagcgg gcccatcgca ttgaatccgt cttcgaactt ttcgcccagt ccgaaacgat
    17041 tcccgatagc tcgatcaatg gcaccaagcg ccatgcagat catcaggatc cacagaataa
    17101 cttcattgat actcatgagg gctccacaac gcctcagaag ccccctgtgg cctcccgttc
    17161 gacgttgagt cccagtatgc caataccccg gcgggcttcg caagagccaa tgtgtggttg
    17221 tgatcaaaat cgccttggcc aattggctag gacgtgtgga gggactattt tcgacatccg
    17281 gtccaccgtc gaaagggcac tctttcgacg gggtgaggca cgatgggggc gtggacgatg
    17341 ccttgctgcc gcgcaacacc gcagccctca gagccctgct cgacaagggc gtgacgatgc
    17401 ccgtgccgtt gaccgtcgag atcggcgacg aggtcgatgt cgagcacgtc agcggcgacg
    17461 gcgtcgtcat ccaccccggc acccggatcc gtggcgcctc gaccgtcatc tccgccggat
    17521 gcgagttggg tcgggagggg ccggtgacga tcgaggactg ccgcctcggt cgcggggtcg
    17581 cgctcaaggg tgggtatgcc gcccgcgcta ccttcctcga tggcgcgaac ctcggtctgg
    17641 gacaccacgt ccgggagggt tgcctgctgg aggaggagtc cggcggagcg cactgcgtcg
    17701 ggctgaagca gacgattctc atgcccttcg tcacgctggg gtcgctgatc aacttctgtg
    17761 actgcctgat ggccggcggc acgagccgca cagagcacag cgaggtcggc tcgtcctata
    17821 tccacttcaa cttcaccccg ggcggcgaca agacgacgcc gagcctcttc ggcgatgtcc
    17881 cgcgcggcgt gctgctgcgc gagccgccga tcttcctcgg cggccagggc ggtgcggtcg
    17941 ggccggtgcg cacggcatac ggcgcggtgg tcgcggcagg atcggtgttg cgcagcgata
    18001 ttcgcgagga gcgctcgttg acgatcgtcg gcccgccacc cgcgatgaca cagcgcgatt
    18061 tcaccggccg ggagtatcgc ggcatcaacc ggctcgtcgc caagaacctc gactacctcg
    18121 ccagcctcga cgccctcgag gcctggtatg ccgaggtgcg cctccccttc ttcgccgccc
    18181 agggggcctt gggggtgctc gtgggggagg gggctcggga ggcgatcgcc gccgggcgcc
    18241 gcgagcggat cacccggctg ctcgcgctgg ccgggcggat ggatcccggg caagcgcccc
    18301 aggctgcctt cgccgcgcac gcgcaggcac tcaccgagat ctttggtggg tcgtatgccg
    18361 ctgtgcccga gtcactttcg acccacgccg cagccaccgc agcggcgggg gcgccctatg
    18421 tcgaggccat ccgcgcgctc ccggacgacc tcgtcaatgc cggccagcac tggctggccg
    18481 gcatcatcga ggaccgctgg gccgacgtcg aggcactcct gccgggcgtt gcccgactgc
    18541 ggcaccgcga aggatgagcg tcggcgacga cgatgtggcc gccgccctgt ctcgcgtcca
    18601 ccgcaccgag tggggccggg tcgtcggggc cctggtcggc gtttcggcga tctcgatatc
    18661 gcggaagacg ctgcggcaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 ggcgcatcac gcgcgccaag gcgaagatca aggatgcggg gattccgttc gcggtgccgt
    18841 cggaggccga cgtgcgcgag cgcctcgacg gcgttcttgc cgtggtcctc ctcatcttca
    18901 acgagggcta tctcgccagt gacggcgcgg acccggtgcg cgtggggctg acggatgagg
    18961 cgatccgccc gcgagcgggg cgcgtggggg aggcgcgggc ggcatacgac cgggcaatcg
    19021 cgctcgccgg caaccccgcc gaacgcgacc ttctcgctcg ccgccgcgac cagctcagcg
    19081 gcgaacgacc ggctcagtcc gacgacgacc cgccggagtc tccgccgcca ccgcccgagt
    19141 ccccaccacc actcgagtcc ccgccccagg acccaccacc ggagtcggac cccccggcat
    19201 cagagccggt ctggggcatc ggggctgtcg gatccgagga gctcggcgac cccgaccaca
    19261 ggctgccacc ggactccgga taggccggcg cctcggtggt ggactgggga tactgcccat
    19321 acggctgctg gggatagggc gacgacggga ccggggccgg ccctggatag gcgggggagt
    19381 tccactgact gcccgcgacc gcgccggccg ccgacccgga tgccgccggg ccataggggt
    19441 agccgccggg ctggtaggcc ggccgcacat agtcctgctg ggggctgcca taagcgtgcg
    19501 cctgcccgcc gaggctctcc cggcgccggg cgtcgatgta catcagcgtc gaatagacca
    19561 ccgaatagag ctggaagata tagagcgcga tggcgccgat gcccatcacg atggcggcga
    19621 cgatgccgcc cgccacggag gaggcgtcgc cggaatcgcg cgtcaacgcg ctgaacggag
    19681 cggaggcgag gctgaagacg aacatcagcg gcagcgccag cagcgacagc atcagctgcc
    19741 ggcccagggt gacccaccac atgcccttgg tgaggacgaa ggtgcgccgg atcgcctcga
    19801 tggggccggc cttctcgatc gcgacggtct gaaagatgaa cgcgcacttg accgaaagcc
    19861 agatcatcac cgggtaggcc aggaagagca gcaacagcat gacgccaatg ccgaagccct
    19921 cgtccgcagc gaccgaacca ccgatgagca ggatcgcgag cacgccgacc accaccgcgg
    19981 caccgacgat cagcagatag aggccgagga cattccagat gatgccgcgg gtctcgttcc
    20041 acagggctcg caaggccggc cgttcgccga gggccacgcg ataggcggcg atgaccagcg
    20101 ccgcgctgat ccggatcagc accacggcga ccgcgaccgc cagcacggcc gcgaggaggg
    20161 ccgtcaccgc cgctgccgcg ctgccgtcgg atgtgctcgc cagcgccacg ccgacgccga
    20221 cgaggatcag caccggcagc agcagggccg cggccacggc caccgggacg agcaggaaca
    20281 ggcgcgggtc cgccgtcagg gtgcgaaagg cgccgctgac gagatcgccg acgctgagca
    20341 gccggttggg gatcgtcggt gcggcggtgc ctcggtatgc cgacgcgtcg ccccccggcg
    20401 tcggctcggg cggatatgtg ctcatgtcgt gcttccctcc atcgtgtcgc ggcacctcgt
    20461 tcgtgccgct gacgtgcgtc atcgtagggc gcgccggcgg tgatcgggac ggcagcgctg
    20521 tgcctcacgc gcgcagtgct gcctcgacat ctttggcggt ggccggctgg gcgcccttct
    20581 gggacgcgcg ggccacgaca tcgggttccg cacgcagcag cgcgacgccg cgtttgatga
    20641 ggataagcgg cggtttgcgg cgctccttca gatcccgcgc cagccgccgg atgaaggtca
    20701 gcgccggccg gttgcgcacg caccaggccg agtgcagcag gccgcgctcc cggagcggag
    20761 cgatgagttc ggcagcgaag atgccttcgg cgagaaccag atccgtcggc cgggccgtaa
    20821 tatcaccctc gccgacgcgc ttggaacggc tgatgtcata gaccggcatc gcgctacagc
    20881 cggtcgtgat gagcgcctcg agcgctgcgc acgccgccgc cccgtcccag ctgtccgggt
    20941 ggtcccagtc ggcgacgtcg agggctgggt gcttcggcag gccgggcgcg tcgaagtcct
    21001 tgtagaagtc gtcgagccgc acgatgggcc agccgtatgc cgcgcggagg cgctccgcga
    21061 gccgggactt gccggctccc gacgggccgg cgagcaggac gacgcgcgcg tggtcgggtg
    21121 tctccggtgg tgtcgtctga ggttcggtgg gcggcggtgg tggcactcag cgacccttgg
    21181 gatgccagac ggtcttggtc tccaaaaagg ttgtcatgcg ggagattgcc ggctcgatgg
    21241 cccagtcggg ttcttcggcg cgcgccccgg cgccgcccgg ccggacgatc agcttgaggg
    21301 tctcggccgc gctggcctcg agttcacccc aggtcaggcc ctcgacgccg gcggtgccgg
    21361 tgaggtcgag gccgttgacg tcgcggtgcg acgcgagcca gggcgcgatc tcggcggcat
    21421 cgccggtgat gatattgacc acgccgccgg gcacatcgga ggtggcgagg cattccgaga
    21481 gcgtcacggc gggcagcggc cggtccttcg aggagacgac gacggcggtg ttgcccgtcg
    21541 cgatcgccgg ggcgatcacg ctgacgaggc cgagcaggct cgactcctgc ggggcgagaa
    21601 tcgccacgac accgcacggc tccggcaccg acagattgaa gaaggggcct gcgaccggat
    21661 tggggttgcc ggcgacctgg gcgatcttgt cggtccaccc ggcataccag acccagcggt
    21721 cgatcgcttg ggagacaagg gaatcggcgc ggctggggct gatcccctcg ccaacgcgga
    21781 cctcctcggc gaattgggcg cggcggccct ccatcacctc ggcgacgcga tagagcacct
    21841 gtccccggtt gtatgccgtg gcacccgccc acccgggctg cgcggcccgc gcggcgacga
    21901 ccgcgtcgcg ggcgtccttg cgcgagccct gcgcagcatt cgccaggaac cggggagtgg
    21961 caccggcgcg cgcgggggcg gcgaacacct catacgaccg gccggactcg ctgcggggga
    22021 acgtgccgcc gatatagagc ttgtaggtct tgcggacatc gagtcgcgcg gccatcgtca
    22081 gttgcttccc ttcaggtagc ccgcgaggcc gtgacggcct ccctcgcgtc cgtagccgga
    22141 ctccttgtag ccgccgaagg ggctcgtcgg atcgaattga ttgaacgtat tggcccacac
    22201 cacaccggct ttgagctggt cggcggtcca caggatgcgg ctgcccttgt cggtccagat
    22261 gccggccgag aggccgtatg gcgtgttgtt ggccttggcg accgcctctt gcggggtgcg
    22321 gaaggtcaag acggagagca ccggtccgaa gatctcctcc tgagcgatgc ggtgagtctg
    22381 ggtgacgccg ctgaagaccg tggggcggaa ccagaatccc ttgtccggca acgggcatcc
    22441 gttgtcccag cgctgcgcgc cctcggcctc accggcctcc gagagttgcg tgatcttgtc
    22501 cagctgggcg cgcgagttga tcgcgccgac gtcggtgttc ttgtcgagcg ggtcaccgac
    22561 tcggagggtg gccaggcgcc gctgcaaccg tcgggtcacc tcctcggcga tcgactcctg
    22621 gacgagcaat cggcttccgg cgcaacagac atggccctga ttgaagaaga tgccgcggac
    22681 gatgccttcg acggtctggt cgatgggtgc gtcgtcgaag acgatattcg cacccttgcc
    22741 gccgagttcg agggtcacgc ccttgcgggt gccggcgatg gagcgggcga tcatcttgcc
    22801 cacccccgtg gagccggtga aggcgatctt gtcgatgtcg gggtggtcga cgatggcctg
    22861 gccggtctcg cgggcgccgg tgacgatatt gacgactccg ggaggcagct cggcctgctg
    22921 gcagacctcg gcgaagagca gcgccgtcag gggcgtggtc tcggccggct tgaggacgac
    22981 ggtgttgccg gttgcgaggg cgggggcgat cttccacgac agcatcaaca gtgggaagtt
    23041 ccatggaatg acttgcccga caacgccata cggctgcggg tctgggccga ggccggcgtg
    23101 gccgagtttg tcggcccacc ccgcgtgata gaagaagtgc gccgcggcga gcgggatgtc
    23161 gacgtcgcgg gtctccttga tcggcttgcc gttgtcgagg ctctcgagga ccgcgaactc
    23221 gcgggcgcgc tcctgcagta agcgggcgat gcggaagagg tacttgccgc ggtcggcgcc
    23281 actcatccgc gaccagaccc ggctgtatgc cgtgcgcgcg gccttgacgg cgaggtcgac
    23341 gtcggaggcg tcgccttcgt ggatgtcggc gaggacctcc tcggaggccg ggttgacggt
    23401 cttgaaggtc gtgccgtggc cgtcgaccca ttcgccgttg atgaacaggc cgtagctgct
    23461 ggcgatgtcg acgacggcgc gggactcggg cgcgggcgcg tattcgaagg tgggcatcgt
    23521 tggtcgcgtc ctcagtaggc ggtcgagggg gaatcgtcgg cgatgtagac ggcgctggaa
    23581 tagacgccgg tccggagctt ttgtcgttgc agcagtaggt cgttgagcaa cgagctggcc
    23641 ccgaagcgga agtagtggtt gtcgagccag tcctcgccgg ccacttcatt gacggcgacg
    23701 aggaacttca ccgcgtcctt gcttgtccgt atgccgccgg ccggcttgac gccgatctgc
    23761 tcgccggtca catcgcgcca gtcgcgcacg gcctcgagca tgatgagcgt gacgggcagg
    23821 gtggcggcgg gggagatctt gccggtgctc gtcttgatga agtcgccgcc ggcgagcatg
    23881 gacagccagc tggcgcggcg cacgttgtca taggtgacca gctcgccggt ctccatgatc
    23941 accttcagcc gcgcggcgcc gcatacggcc ttgaccttgg cgatctggtt gaacacctcg
    24001 agatagttgc cggagaggaa ggcgccccgg tcgatgacca tgtcgatctc gtccgcgccg
    24061 gctgcgacgg cgtccttggt gtcgagcagc ttgacgggca cgctcgcgcg gccactgggg
    24121 aaggcggtgg cgaccgcggc aaccttgacg cccgaatcgc cgagggcttc cttggccgcg
    24181 gcgaccatgt cgccgtagac gcagaccgcc gccgggcgcg gggtgctcag gtcggtcggg
    24241 tcaggggtca gggctttggc cgccagcccc cggacccggc cgacggtgtc ggcgccctca
    24301 agggtggtca ggtcgatcat cgagatcgcc aggtcgattg cccagcgctt gctgtcgttc
    24361 ttgatggagc gagtgcccag ccctgccgcc cgcgcctcgc agcccacctg gtcgacgccc
    24421 ggcaagccat gcagccaggt cgtcaggccg gcattggtca gccgcgtcac cccgaggaag
    24481 tcgcgtgctc gggcggtggc gtcgacggca gcgtcggctg gggcagaact caccgtggga
    24541 gtctatccgc ggcgcgtggc ggcgagccct cgttgcgccc cccggacatg acgaagcccc
    24601 cgggaggggg gccgggggct tcgtcgggac agaaggctca gcgcaggacg ccgttgccga
    24661 ccttgcggcc ccaccacttg ccgatgccgt ctcgccggcg agaccggagg cgcacagcag
    24721 gagcagcgcg gcctcgtgcc agtgcgagtc agtgatgggg ttggtggcgt ggaagtcagc
    24781 aggctgaccc tgcgggaact
//
LOCUS       DAOPJG010000019        24658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2137_length_24658_cov_7.654514, whole genome shotgun sequence.
ACCESSION   DAOPJG010000019 DAOPJG010000000
VERSION     DAOPJG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 24658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24658
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2137_length_24658_cov_7.654514"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PKU75_03460"
     CDS             complement(<1..807)
                     /locus_tag="PKU75_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF79963.1"
                     /translation="MSDFDVVVLGAGPGGYVAAIRASQLGLKAAVVEKKYWGGVCLNV
                     GCIPSKALIKNAELAHTLTHDKAKYGIEGEVTMSFGPTHARSRQVSAGIVKGVHFLMK
                     KNKIEEIDGWGTLTSPTSMSVALNSGETREITFDNLIIAAGAVTRMLPGVQVSQNVVT
                     YEEQILSDQLPGSIIIAGSGAIGVEFAYVMKNFGVDVTIVEFLDRMVPTEDADISKEL
                     LKHYKKLGVKVLLGTKVESVEDTGSGVRVTVSPAAGGEQQILEADKLLSAI"
     gene            complement(861..2111)
                     /locus_tag="PKU75_03465"
     CDS             complement(861..2111)
                     /locus_tag="PKU75_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79964.1"
                     /translation="MRARLLILSSAVAMLGWGAVLPYQYAYAASARGWGGLVAAAAST
                     LFSVGALVAAPVGGRLADRVDPVAVAVVAKLAAAVAMAALVAADAPATFLFGMFAFGL
                     GVTAAMPAQSVLALRWTGDADRRRVFAWVFTAGSLGMAIGAFVAGYLVDLDRPDGMTG
                     AFLIAAAGFALSGLLIAVAGHRAPAPTAYDLGDDPARPLGGLHALRAIWAVPALRWTA
                     VVTVTLALGFYAQFESGLPAYALTSLDVAERTVGLAAAVNCVVIIALQMVVVRLTAGH
                     SGAGLLMIVGGIWTLSWVLLSAALFTPAVAGAVFVTSFAVFAVGETMYAPILNPLTAS
                     LAPEGMVGTTLGVFTAVQTGASALGPLVAGVLLGAGMTGGFLGLHLLISLGAIVAAAR
                     LRAVIRRPAETGAVPEPEVLTLAA"
     gene            complement(2198..2692)
                     /locus_tag="PKU75_03470"
     CDS             complement(2198..2692)
                     /locus_tag="PKU75_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPF79965.1"
                     /translation="MSGARAEYQAAVDAYVAAGGDASVQRAITAISQVTKKLDQWYDR
                     QLADLGISHGDWSVLSQLAKADGGPLTPSLLADAANLAPSSMTSRLDRMCERGLVVRE
                     PDQVKRTRVLVTLTEAGWELFAQAIREANVVESDVLADLRPEQRTTLADLLEVVLEGL
                     DKHT"
     gene            complement(2704..3123)
                     /gene="ndk"
                     /locus_tag="PKU75_03475"
     CDS             complement(2704..3123)
                     /gene="ndk"
                     /locus_tag="PKU75_03475"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPF79966.1"
                     /translation="MTERSLVLVKPDGFKRGLTGEVLARIERKGYRLVALQILTPTRE
                     ALAEHYAEHVGKPFYEPLVEFMSSGPVTAVVIEGEQCIAGFRSLAGATDPTKAAPGTI
                     RGDFGRDWGTPAQENIVHGSDGPESATREIGIWFPAL"
     gene            complement(3148..3540)
                     /locus_tag="PKU75_03480"
     CDS             complement(3148..3540)
                     /locus_tag="PKU75_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HPF79967.1"
                     /translation="MSWLRAPLGKFTWRMLATVLFGQGLSVLLGATLARSVAATTGEG
                     DPGGQLWAGLALGLLCFAAAGGMRRGWGLPLGWVAQVLTLASALIVPLMLWVGLMFLA
                     LWIYCLRKGPQIDAAVAARDAEAATPSA"
     gene            complement(3604..4563)
                     /locus_tag="PKU75_03485"
     CDS             complement(3604..4563)
                     /locus_tag="PKU75_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPF79968.1"
                     /translation="MEERQLGNGGPSVSVIGLGCNNFGRVGRATQSLEGTKAVLDAAL
                     DAGITLLDTADMYGSPATGSETLMGEALAGRRDQIVLATKFGHADVTLPGTQEWGPKG
                     GRTYIRNACEASLTRLRTDHIDLYQQHTPDPSVPIEETLGALTELVQEGKVRYIGHSN
                     FSAAEAVAAEAASAGAGGARFVSAQNEFNLLARTAERDLLPTLRRLGVGLLPYFPLAN
                     GLLTGKYSRDHRPEGTRIVDDKPQLLESVDWDRLAAYERICQEAGAPMSQVTFAWLLS
                     RPGLVSVIAGATSPGQVAENAAAASVRLPADLIAAIDQLFPIA"
     gene            complement(4581..5969)
                     /locus_tag="PKU75_03490"
     CDS             complement(4581..5969)
                     /locus_tag="PKU75_03490"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPF79969.1"
                     /translation="MTPAPDAQAREAAYQLDLTKRMREAEQAILTRAPEHDLEPDLTR
                     ISAVMELLGDPQHSFPVIHITGTNGKTSTARIITQLLTTSGLTVGRFTSPHLHTLRER
                     ITIGGEPISVEAFLATYDEVLPLIEIVDARSQADGGPRMTYFEVLVAIGYAAFADAPV
                     DVAVVEVGMGGGWDATNVADGQVAVVTPIAMDHAHFLGNSLEAIAGEKAGIIKPDALA
                     VIGFQEPEVVPNLAERAEEVGARMAFEGNDFGVLSRDVAVGGQQVSLRGLAGDYNDLF
                     LNLHGAHQAHNAAISLAAVEAFLGGGQQPLNSDVVREAFAAVTSPGRLEIVRRSPTVI
                     VDAAHNPAGAQVLATALAEEFNFTKRVGVLAILADKDAEHMLEALEPVLDEVVVTRST
                     SPRAMAPEKLGELAREIYGDHRVVVVNDLPAALDQAAELADEGGVGGGVIATGSVTTA
                     ADVRLLLGAKDA"
     gene            complement(6006..6314)
                     /locus_tag="PKU75_03495"
     CDS             complement(6006..6314)
                     /locus_tag="PKU75_03495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPF79970.1"
                     /translation="MDENTGPVALIAEFTTTQERLDTVRGLIAAYGEVVRAEPGNRAF
                     DVYTHVAEPTKFWVHEIYADRAAFEAHIGDPAGTEFNGKLVPLIVEPASVLTFLTPIA
                     "
     gene            6477..8756
                     /locus_tag="PKU75_03500"
     CDS             6477..8756
                     /locus_tag="PKU75_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HPF79971.1"
                     /translation="MSTQVEERGDGVRVYRNDPNLPPIAVEEPHTITPKGKLIFGLIG
                     LLGAIGWAMIALVRGENVNAVWFVVAAICTYIIAFRFYARLIEYKVLQPRDTRMTPAE
                     EFENGKDFMPTDRRILFGHHFAAVAGAGPLVGPVLAAQMGYLPGTMWIIIGVVFAGAV
                     QDYMVMHMSIRRRGRSLGQMARDELGPVGGWAAILGVLTIMIILIAVLGIVVIGALAE
                     SPWGVFSVGLTIPIALFMGVYLRYIRPGAVNEVSLIGVALLILAILAGGWVHEHDTLS
                     QTFTLSKETLAWLLIAYGFCAAVLPVWLLLAPRDYLSTFMKVGTIALLALGILIARPV
                     VKMPAVTEFASTGTGPAFAGSLFPFLFITIACGALSGFHALISSGTTPKLIEKESQAR
                     MIGYGSMLTESFVAVMAMVAAVSIDQHIYFAMNAPAGLTGGTPETAAAYTNGLGLVGP
                     GGAALANIDPALLSQAATDVGEKSIISRTGGAPTLAFGMSEVMSAFGGAGMKAFWYHF
                     AVMFEALFILTTVDAGTRVARFMFSDAIGNLPGLGKFKDPSWRVGAWICSLIVVAGWG
                     SILLMGVRDPLGGIYTLFPLFGIANQLLAAIALAVVTTILIKQGKAKWAWIPGIPLAW
                     DLLVTLTASYQKIFSDVPAIGYFANHDKFVAAKEGGKILAPAKTMEDMDKVIRNTTIQ
                     GWLSIIYAVLVIIVVLAALAVMLKALRSGGLPTSEAEDVPSKIFAPRLFLPTKAEQDV
                     LDEWKEAGLDPTPVGAHHG"
     gene            8768..8941
                     /locus_tag="PKU75_03505"
     CDS             8768..8941
                     /locus_tag="PKU75_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HPF79972.1"
                     /translation="MSRSLRWFWSGVTGADAYDRYCAHLQRVHPGCPLPTKSDFWREK
                     YAEMERNPKSRCC"
     assembly_gap    8971..9070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9071..10067)
                     /locus_tag="PKU75_03510"
     CDS             complement(<9071..10067)
                     /locus_tag="PKU75_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPF79973.1"
                     /translation="MAFAVADALGQVRLSEQRHPDAITYSVLEELIAEQLATDELIRG
                     IALGIPGVARDGVVEDCDVDALVGVDVAGGVRDRFGINPEVRNDMDFIATGVYTSTPH
                     NGGSLAAMLFPEDGYVGCGFVIDGAVLQGHSGFAGELYRIAEGFGIDRQAQLNALQDR
                     HDFRELAAKAALIVCCTFDPEVIVLLGHGMDTDDLQAIRLLISNAVTESHVRHARRDR
                     VDLRRSHLRLGDDRQPCRGQPASSGCGVRAVSRSTAVPFDWSHEAPWPAVLEGANAIY
                     VIVDESPHGVDRLGRFLDLSNARGVQRIVLLSARDWIDAASCTRPGTAGVPSSTRA"
     gene            complement(10426..13749)
                     /gene="ileS"
                     /locus_tag="PKU75_03515"
     CDS             complement(10426..13749)
                     /gene="ileS"
                     /locus_tag="PKU75_03515"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPF79974.1"
                     /translation="MAYPKASTTGAGAVPASPRFPAIEEAVLKYWDADGTFQASIENR
                     PAGENGDNEFVFYDGPPFANGLPHYGHLLTGYVKDIVPRYKTMRGMRVERRFGWDTHG
                     LPAELEAMSQLGIKTKDEILDLGIEAFNAKCRESVMKYTHEWQDYVTRQARWVDFDND
                     YKTLNPTFMESVLWAFKSLYDKGYVYEGFRILPYCWNDQTPLSNHELRMDDEVYKIRK
                     DPAVTVGYRLASGPYAGALALIWTTTPWTLPSNMGIAVGPDVDYVVVESEATGRTERY
                     VLAAARLASYANDLWPGKAEDAVATRKDPSSRIVARLKGSDLVGSTYTPPFPYYVGYA
                     GAHQVLGEDFVTTEDGTGIVHMSPGYGEEDKAALDKVGVETVLTVGPDGTFIFPVTAY
                     EGVHVFDANPLITEHLKARTFVDGAGGQADPGPVTEGTVLLRKETYEHSYPHCWRCKQ
                     PLLYMAVSSWFVEVTKFKQRMMELNQEIDWTPDNVKDGQFGKWLENARDWSISRNRFW
                     GSPIPVWRSDNPEYPRLDVYGSFAELEADFGVLPRNEKGEPDLHRPFIDELTRPNPDD
                     PTGQSTMRRVEDVLDVWFDSGSMSFAQVHYPFENREWFEHHFPGDFIVEYIGQTRGWF
                     YTLHILATALFDRPAFQSCISHGIVLGDDGQKMSKSLRNYPDVSEVFDRDGADAMRWF
                     LMASPILRGGNLIVTEEGIRAGVRQALIPLWNAWYFFALYAGAAGYDAQWRTDSTDGL
                     DRYILAKLREYVADSGAAQDVYDIPRACELTRSFLDVLSNWYIRRSRDRFWAAGGTVT
                     QSSKDAFDTLYTVLEVVCRVAAPLLPLVTEEIWRGLTGGRSVHLTDWPNIYDLPEDHT
                     LVAAMDRVHTVCSVGSSLRKAERLRTRLPLSSLTVVTADAAALRPFSAVIADELNVKS
                     VDVRDVADAHQSDFGISQKLTVNARAAGPRLGKDVQVAIKGSKSGDWSIGDDGTVIAG
                     GLPLVEGEYALETVVDTDGGGSVATGLLPGGGFVVLDTDVTLELELEGVARDLIRAVQ
                     GARRDAGLDVSDRIALIVTGGDFVNRAMEEHRDLIKGETLTSILEMSGDLDALTPGNP
                     GVVEATVGDRYPVRLLIRR"
     gene            14105..14710
                     /locus_tag="PKU75_03520"
     CDS             14105..14710
                     /locus_tag="PKU75_03520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF79975.1"
                     /translation="MGDLTIRPANETTPGDIQVVFGTRGSACDCQCQRYKLARGEAFG
                     KFPVEERRRRLEEQTGAGDPDAEATSGLLAYLDREPVGWCAVEPRANYHGMARNQKVP
                     WAGRTEDKSDPSIWAITCVFTRTGFRGKGIAHALVAAAVEHARAGGAAAVEAYPIVTT
                     PGARITWDEIHPGTPQMYAAAGLVEVAHPFPRRLVMRRDFP"
     gene            complement(14707..15045)
                     /locus_tag="PKU75_03525"
     CDS             complement(14707..15045)
                     /locus_tag="PKU75_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPF79976.1"
                     /translation="MDEAAVERVLRVVELIPRGEIAAYGEVGQIAGVGPRQVGAIMSR
                     YGGGVAWWRVTNAYGDPPMHLRDEAFAHWREEGIAIKPNGLGCPIAEFGTDQRVLEQR
                     YAAHSPPPAG"
     gene            15104..16180
                     /locus_tag="PKU75_03530"
     CDS             15104..16180
                     /locus_tag="PKU75_03530"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPF79977.1"
                     /translation="MRVAVLGAGSWGTALGYALATKGYDVAMWDIDMPVLQSITAHEN
                     TRYLPGITLPSSMHGEPELKAALARAQLVVFAVPSQAMRSVGTQAAPLIRDKALLCSV
                     TKGIEVDTLMTMSEVLQDVLPQRLHSRLTFLSGPSFAVEVAREFPTAVTIAGEDAEVT
                     SQVQRAFHTPFLRPYVSDDVVGVEIAGCAKNVIAIACGAGDGLGYDANTRAATITRGL
                     AEITRLAVARGANPLTLLGLAGVGDLVLTCSSVKSRNYRVGFGLGQGRRLDEVQAELG
                     QVAEGVLNAKSVKALAERAGVEMPLCGMVYAVLYEGRDMADLRSFFAERPLKSEPEHH
                     FGRWTRRFRRRGRRVAAGPASPDA"
     regulatory      16343..16417
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            16512..18077
                     /locus_tag="PKU75_03535"
     CDS             16512..18077
                     /locus_tag="PKU75_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79978.1"
                     /translation="MHRPPLTVAASAVALACALTIGAAPGAVADPSAGASDGAAFLAR
                     ELAASGDVLDYPGTDQDDIGVTIDALLGMLVVRSGKTQVERSSDAVAANLDDYLGPTF
                     GATELYAGPVAKSMLLAAATGRDVHAFGGHDLVADLGGLEAADGRYADRTAYGDTSNT
                     FTQAFGVLAMTRAGGGASEKAVNFLVAQQCPDGGLRMVPGTDPCVSDPDATSLAVAAL
                     LGAGGREAEAASALDYLSGAQTSAGGLGGGTGATGVNSNSTGLAGMAFRLGGREAEWA
                     LANDYLGGLQLGCDAPESVRGAVAYDQAGYDALVAEGGGAVAGDQERRATAQAVLSFG
                     PDGLLDASVDLATDAAGDCSDSPSSSSTSSTSSTSSPPTSGTPSQTTTPTTTTPAPTA
                     ATVTATVTVTTTASRPAPPAPPATRTVTVTPDHERAPTRAATSSSSPAASLSSTEAAT
                     SPTGTSTPPPASATASPAAPSADPRAAVTAAATSGAATTSSTGPAIAAAGGLVLAGAI
                     GAAAVLRRGRKSP"
     gene            18074..18541
                     /locus_tag="PKU75_03540"
     CDS             18074..18541
                     /locus_tag="PKU75_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79979.1"
                     /translation="MKSRLLTPLAALTAAAPLAFGTAPSAHATSGTALAICPRGSGVT
                     VIVDFGSWDKRSCAPGDPTTGLAALRGAGFTVTMVQRFPGAVCRINGRPSATVDKCVV
                     MPPTTGYWSYWHAVRGGKWTYSTSGPGSYNPKPGTVEGWSYGAGKPPTAPPPA"
     gene            complement(18907..19278)
                     /locus_tag="PKU75_03545"
     CDS             complement(18907..19278)
                     /locus_tag="PKU75_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79980.1"
                     /translation="MAELDELVDRLPLVDVAEVDGREVVADAEEVVRVPAGPAAPVEE
                     PGVDEPGSAAEVCCAPAVSECADVVPDEVGALVVDVVAGAVVLGGDTTGGAMPGCRPA
                     PKATPTELPGAGFRLAAPVLL"
     gene            19473..20564
                     /locus_tag="PKU75_03550"
     CDS             19473..20564
                     /locus_tag="PKU75_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HPF79981.1"
                     /translation="MIRHLHPAAWWLWALALAAGASMTTNPVLLAAHVGVLVAVVLAR
                     RDGSPWGRAFRLYAVLGVVIVLVRLVLHALVGLKFGDIPLLPLPEAHLPSWAAGIQLG
                     GTVYLEGALGAGLEGARLATMILAIGAANALASPKRLLKVFPAALHEIGAALVVAVTV
                     APQLAGSVQRVARARQLRGEPALRLKGFRSIAMPVLQDTLDRSLALAAAMDGRGYGRR
                     PESTNGRATGALTLLGLLGSCVGIYGLLDTTSPAALGLPTLVVGLVLCVAGLWSGGRS
                     VPRTIYRRDPWSAPEWLTIACGAAALVAMLVTVRRDPAALGMPLAPLGLPALPVLPLA
                     GLALAAVPAVATPVPPLVSARRAPREVAA"
     gene            20561..22264
                     /locus_tag="PKU75_03555"
     CDS             20561..22264
                     /locus_tag="PKU75_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF79982.1"
                     /translation="MTAYDPAISLSHVTIHYPDAAEAALRDVDLEIEEGELVLVIGRT
                     GSGKSTLLGTLNGHVPHFTGGRLSGRVRVLGRDTATHRPRDLADVIGVVGQDPLAGFV
                     TDTVEEELAYGMEQLALPPATMRKRVEETLDLLGIADLRAAPLRDLSGGQQQRVAIGS
                     VLAAHPRILVLDEPTSALDPTGAEEVLATLTRLVHDLGVTVVVAEHRLERVVQYADRV
                     VEVVDGTVRDGIPSAIFETASVAPPVLDLGRYAGWSPLPVTIRDARRAAAPLRARLGD
                     QGTSASRGWSNQQTSLGVQISLTSASRGSSASPGVRARGVRVDYGGVRAVADVDLDLP
                     AGRVTALMGRNGAGKSSLLWALHGAGTRTAGRVSLADGRDPAGLKPGVRRGAIGLVPQ
                     TATDLLYLDTVAAECRQADTESSRDSGTCARLLEELAPGIPGDRHPRDLSEGQKLALV
                     LAVQLTAGPQVLLLDEPTRGLDYPAKARLADTLRGLADHGHTVVVSTHDVEFVAESAD
                     EVVVMAEGEVVAQGPTAEIVAASPAFAPQVAKILAPVPLLTVAQVRAALAERAAPQDT
                     V"
     gene            22261..23100
                     /locus_tag="PKU75_03560"
     CDS             22261..23100
                     /locus_tag="PKU75_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPF79983.1"
                     /translation="MTPASAVRLRPRSVLAIAVVSVAGLLAFGWPLIIDPGSGIAHGG
                     DAPFVFALLLPLLLAVVLAEISEGGLDVKAVAMLGVLSAVGAVLRPLGAGSAGVELVF
                     FLLVLAGRVFGAGFGFVLGATTLFASALITGGVGPWLPYQMLGAAWVGLGAGLLPRAT
                     GRAEVALLAAYGALAGLAYGWLLNFSFWPFAVGPGTGLSFVAGDPATANLHRFVLFSI
                     ATSLGWDLGRAITNAVLIAVTGRAVLGALRRVAHKAAFDATPEFSRAPEASAMVETRD
                     EPS"
     gene            23087..23626
                     /locus_tag="PKU75_03565"
     CDS             23087..23626
                     /locus_tag="PKU75_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase family protein"
                     /protein_id="HPF79984.1"
                     /translation="MSPADVERQESWLSQEDLATVRGRMPILYVEAVPVRVAADGSVT
                     AVGLLLRASDNGRIQRELVSGRVLFHERIRDALLRHIEKDLGAMALPQIPPSPVPFTV
                     AEYFPTPGITAFHDSRQHAVALAFIVPVAGDCAPQQDALDLAWLTPQQAADPAIIADM
                     DGGHGVLLRHALAHLGHAI"
     gene            23765..24304
                     /locus_tag="PKU75_03570"
     CDS             23765..24304
                     /locus_tag="PKU75_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HPF79985.1"
                     /translation="MTTLPRSRPLTRADLAAMPEDGHRYELIDGVLIVTPGPVTHHQR
                     ASRQLLLALSRDVPADLEVFHAPFDVVLADDTVLQPDIVVARQADLTAANLPAAPVLA
                     IEILSPSTRHIDLHLKKSRLEEAGCAAYWVVDPGEPSITAWELRGGRYVEVGHAVGTE
                     VLSVRQPYAVTIRPADLVS"
     gene            complement(24358..>24658)
                     /locus_tag="PKU75_03575"
     CDS             complement(24358..>24658)
                     /locus_tag="PKU75_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79986.1"
                     /translation="MKPAHHIDESPWPERAWLENLLGPRTFVDVVRRLHPDVAGPYTW
                     WSWLGTTYAADAGWRIDFHFASPGLARTARRTWVDREVDGIRMSDHAAVTVDYDC"
BASE COUNT         3664 a         8674 c         8362 g         3858 t
ORIGIN      
        1 gatggccgag agaagcttgt cggcctcgag gatctgctgc tcccccccgg ccgcgggcga
       61 aaccgtcacg cgcacaccgg atccggtgtc ctcgaccgat tcgaccttgg tgcccagcag
      121 gaccttgacg ccgagcttct tgtagtgctt gagcagctcc ttggagatgt cggcgtcctc
      181 ggtcggcacc atgcggtcga ggaactcgac gatcgtcacg tcgacgccga agttcttcat
      241 gacataggcg aactcgaccc cgatggcacc ggagccggcg atgatgatcg agccgggcag
      301 ctggtcggag aggatctgct cctcataggt gacgacgttc tgcgagacct ggaccccggg
      361 cagcatccgg gtgacggcgc cggcggcgat gatcaggttg tcgaaggtga tctcgcgggt
      421 ctcgccggag ttgagcgcga ccgacatgga cgtcggcgag gtgagggtgc cccacccgtc
      481 gatctcctcg atcttgttct tcttcatcag gaagtggacg cccttgacga tgccggccga
      541 gacctgtcgc gagcgggcgt gggtcgggcc gaaggacatc gtcacctccc cttcgatgcc
      601 gtatttcgcc ttgtcgtggg tgagggtgtg cgcgagttcg gcgttcttga tcagcgcctt
      661 gctcgggatg cacccgacgt tgaggcagac accgccccag tacttcttct cgacgacggc
      721 ggccttcagg cccagctgtg aggcgcgaat cgccgcgaca tatccaccag gaccggcacc
      781 gaggacgaca acatcgaagt cagacatggg tccaacccta gtgcggatcg gagccggccg
      841 tggtccggcg tccaggcccg tcaggccgcg agcgtgagca cttccggctc cggcacggcg
      901 cccgtctcgg cggggcgccg gatgacggca cgcaagcgcg ccgcggccac gatcgccccg
      961 agcgagatca gcaggtgcag accgaggaag ccgcccgtca tacccgcccc cagcagcaca
     1021 ccggcgacca gcggccccag cgcggaggca cccgtctgga cggccgtgaa gacaccgagc
     1081 gtcgtgccga ccattccttc cggtgccagg gaggcggtga gcgggttgag gatgggggca
     1141 tacatcgtct cccccacggc gaagacggcg aagctggtca cgaagacggc gccggccacc
     1201 gccggagtga agagcgcggc ggacagcagc acccagctca gggtccagat gcccccgacg
     1261 atcatcagca ggccggcacc gctgtggccg gcggtgagcc ggacgacgac catttgcagg
     1321 gcgatgatga cgacgcagtt gacggcggcc gcgaggccga cggtgcgctc ggccacgtcg
     1381 aggctggtca gggcgtaggc cggcaggccg ctctcgaact gcgcatagaa gccgagcgcg
     1441 agggtcacgg tcaccactgc ggtccagcgc agggcgggca cggcccagat ggcccgcagg
     1501 gcgtggaggc cgccgagggg gcgggccggg tcgtccccca ggtcgtatgc cgtgggcgcc
     1561 ggcgcccggt gacccgccac ggcgatcagc agccccgata gcgcgaatcc ggctgccgca
     1621 atcaggaaag cacccgtcat accgtcaggg cggtcgaggt cgacgaggta gccggcgacg
     1681 aaagcgccga tcgccatgcc cagcgaaccg gcggtgaaga cccacgcgaa gacccggcgg
     1741 cggtcggcgt cgccggtcca gcgcaacgcg aggaccgatt gcgccggcat ggccgccgtg
     1801 acaccgagac cgaaggcgaa catcccgaac aggaaggtcg cgggtgcatc ggcggcgacg
     1861 agggcggcca tcgccacggc cgccgcgagc ttggcgacga ccgcaaccgc gacagggtcg
     1921 acccggtcgg ccaaccgacc gccgacgggc gcggcgacga gggcaccgac ggagaagagg
     1981 gtcgaggccg cggccgccac cagcccaccc cacccgcgcg ccgaggcggc atacgcgtac
     2041 tggtagggca gcacagctcc ccagccgagc atggctacgg cggaggagag gatgagcagg
     2101 cgggcacgca tatctggatc aacaatcgtt cgatactaaa tatttcggac tcgaaatctt
     2161 agctatcgaa ccttgcagtg gccaaacccg ttccggctca ggtgtgcttg tccaggccct
     2221 ccagcacgac ttccagcagg tcagccagcg ttgtccgctg ctcggggcgc aggtcggcaa
     2281 ggacgtcgga ttcgacgaca ttggcctccc ggatcgcctg ggcgaacagc tcccagcccg
     2341 cctcggtgag cgtgacgagg acccgggtgc gcttgacctg gtcgggctcc cgcaccacca
     2401 gtccgcgttc gcacatccgg tccagacggc tggtcatcga cgacggggcg agattggccg
     2461 cgtcggccaa cagggacggc gtcaggggcc cgccgtcggc cttggccaat tgcgagagca
     2521 cggaccagtc accgtgcgag atcccgagat cggcgagttg gcggtcatac cattggtcga
     2581 gcttcttggt gacctggctg atcgcggtga tggcgcgttg caccgacgcg tcgcccccgg
     2641 cggccacata ggcatcgacg gccgcctgat actcggcgcg ggccccgctc atgaccgcct
     2701 gccctagaga gccgggaacc agattccgat ctcgcgcgtg gccgactcgg gaccgtccga
     2761 gccgtgcacg atgttctcct gcgccggtgt gccccagtcg cgcccgaagt cgccacgaat
     2821 ggtgccgggc gccgccttgg tcgggtcggt cgcccccgcc aggctgcgga agccggcgat
     2881 gcactgctcc ccctcgatca cgaccgccgt cacgggaccc gaggacatga attccaccag
     2941 cggctcgtag aagggcttgc cgacatgctc ggcgtagtgc tcggccagag cctcccgcgt
     3001 cggggtgaga atctgcaggg cgacaagccg atagcccttg cgctcgatgc gggccagcac
     3061 ctcaccggtg agaccccgct tgaaaccgtc gggcttgacg aggacgagag agcgttcggt
     3121 cacgcccctc agactagacc cgcgcggtca agcggacgga gtggccgcct cggcatcccg
     3181 cgcggcgacg gccgcgtcga tctgcggccc cttgcgcagg cagtagatcc acagcgccag
     3241 gaacatcaga cccacccaca gcatcagcgg cacgatcagc gcgctggcga gggtgagcac
     3301 ctgcgcgacc cagccgagcg ggaggcccca gccgcgccgc ataccacccg cggcagcgaa
     3361 gcagaggagg ccgagcgcga ggcccgccca cagctggcca ccggggtcgc cctcccccgt
     3421 cgtcgcggcg acggagcgcg ccagcgtcgc cccgagcagc acgctcaggc cctggccgaa
     3481 gagcaccgtc gcgagcatcc gccaggtgaa cttgccgagg ggcgcgcgaa gccaggacat
     3541 gggcgacagg gtatgccgcg aaaccccgcg acgtgtcccc acccgtggtg tgtggtggcc
     3601 cgatcaggcg atagggaaca gctggtcgat cgcggcgatc aggtcggccg ggaggcgcac
     3661 gctcgccgcg gcggcgttct ccgccacctg cccgggcgac gtggcgccgg cgatgacgct
     3721 caccagaccg ggccgcgaca gcagccaggc gaaggtcacc tgactcatcg gcgcgccggc
     3781 ctcctggcag atccgctcgt aggcggcgag gcggtcccag tccaccgact ccagcagctg
     3841 cggcttgtcg tcgacgatgc gagtcccctc gggccggtga tcacgcgagt acttgccggt
     3901 gagcagaccg ttggccagcg gaaagtatgg gagcaggccc actccgaggc ggcgcagggt
     3961 gggcagcagg tcgcgttcgg cggtgcgcgc caacaggttg aactcgttct gcgccgagac
     4021 gaagcgtgct ccgcccgcgc cggcgctcgc tgcctccgcg gccaccgcct cggcggccga
     4081 gaagttgctg tgcccgatgt aacgaacctt gccctcctgc accagctcgg tcaacgcccc
     4141 gagggtctcc tcgatcggga cgctcgggtc gggtgtgtgc tgctggtaca ggtcgatgtg
     4201 gtcggtgcgc agccgggtca aggacgcctc gcaggcgttg cggatgtaag tccgcccacc
     4261 cttggggccc cactcctgcg tgccgggcag ggtgacgtcc gcgtgcccga acttcgtcgc
     4321 caggacgatc tggtcgcggc gcccggccag tgcctccccc atcagggtct cgctgccggt
     4381 ggcgggcgag ccgtacatgt cggcggtgtc caagagcgtg atgccggcgt cgagggcggc
     4441 gtcgagcacc gccttggtgc cctcgaggga ttgcgtcgcg cggcccacgc ggccgaagtt
     4501 gttgcagccc aggccgatca cgctgaccga cgggccgcca ttaccgagtt ggcgttcctc
     4561 catgcgacga ccctatgcag ttatgcgtct ttcgcaccca gaagcaggcg gacgtccgcg
     4621 gcagtggtca ccgacccggt ggcgatgacg ccgccgccga caccgccttc gtcggcgagt
     4681 tcggcggcct ggtcgagtgc ggcgggcaga tcgttgacca cgacgacgcg gtggtcacca
     4741 tagatttccc gtgccaactc cccgagcttc tccggcgcca tggcccgcgg cgacgtcgag
     4801 cgggtgacga cgacctcgtc gagcaccggt tcgagggctt cgagcatgtg ctcggcgtcc
     4861 ttgtcagcga ggatggccag caccccgacg cgcttggtga agttgaactc ttcggcgagc
     4921 gcggtggcga gcacctgcgc gccggccggg ttgtgcgcgg cgtcgacgat gaccgtgggg
     4981 ctgcggcgca cgatctccag ccggcccggg gaggtcaccg cggcgaaggc ctcgcggacg
     5041 acgtcactgt tgagcggttg ctggccgccg ccgaggaagg cctcgaccgc cgcgagtgaa
     5101 atagcggcgt tgtgggcctg gtgggcccca tgcagattga ggaagaggtc gttgtagtca
     5161 cctgcgaggc cgcgcaggct cacctgctga ccgccgacgg cgacgtcgcg agataagacc
     5221 ccgaagtcgt tgccctcgaa cgccattcgt gcgccgacct cctcggcccg ctcggcgagg
     5281 ttgggcacga cctcgggctc ttggaacccg atgaccgcga gtgcgtccgg cttgatgatc
     5341 ccggctttct cccccgcgat cgcctcgagg gagttgccga ggaagtgcgc gtggtccatc
     5401 gcgatcgggg tgacgaccgc gacctgcccg tcggcgacat tcgtcgcgtc ccagccaccc
     5461 cccataccga cctcgacgac ggcgacatcg acgggggcat cggcgaatgc ggcatacccg
     5521 atcgcaacca gcacttcgaa gtacgtcatc cgcggaccgc cgtccgcctg gctgcgcgcg
     5581 tccacgatct cgatcagcgg cagcacttcg tcgtacgtcg cgaggaaggc ctcgacggag
     5641 atcggctcac cgccgatggt gatgcgctcg cggagggtat gcaggtgcgg cgaggtgaac
     5701 cggccgaccg tcaggccgct ggtggtcagc agctgcgtga tgatccgcgc cgtcgaggtc
     5761 ttgccgttgg tgccggtgat gtggatgacc gggaagctgt gctggggatc gccgagcagc
     5821 tccatgaccg cgctgatgcg ggtcaggtcg ggctcgaggt cgtgttcggg cgcgcgcgtc
     5881 aagatggcct gctcggcctc gcgcatgcgc ttggtcaggt cgagttggta tgcggcctcg
     5941 cgggcctggg cgtcgggtgc cggagtcacg gggccgattc tctcagcccg cgggcgggtg
     6001 gccggtcagg cgatcggtgt gaggaaggtc agtaccgacg cgggctcgac gatcagcggc
     6061 acgagcttgc cgttgaactc cgtgccggcc gggtcgccga tgtgggcctc gaaggcggcc
     6121 cgatcggcat agatctcgtg cacccagaac ttcgtcggct ccgcgacgtg ggtgtagacg
     6181 tcgaaggcac gattgccggg ctcggcccgc acgacctcgc cgtaggccgc gatcagcccc
     6241 cgcaccgtgt cgaggcgctc ctgggtggtg gtgaattccg cgatcagggc aaccggtccc
     6301 gtgttctcgt ccacgtcgcc acgctattcg tccccgcggc atccggccca ccagcgtgac
     6361 ggataggtca caaagtagaa acgggtgtga caggtgtgcc tgaaggtgcg tgacggggag
     6421 gccgggccga gagtaggttg cggccattgg cgcacgtaag gacagggagg tccgcgatga
     6481 gtacgcaggt ggaggaacgc ggcgacggag tgcgggtcta tcgcaatgac ccgaacctgc
     6541 cgccgatcgc ggtcgaggag ccgcacacca tcacccccaa gggcaagctg atcttcggcc
     6601 tcattggcct gctcggcgcg atcggctggg cgatgatcgc tctggtccgt ggagagaacg
     6661 tcaacgccgt gtggttcgtc gtcgcggcga tctgcaccta catcatcgcc ttccgcttct
     6721 atgcccgcct catcgaatac aaggtgctgc agccacgcga cacccggatg acaccggccg
     6781 aggagttcga gaacggcaag gacttcatgc cgaccgaccg gcgcatcctc ttcggtcacc
     6841 acttcgcggc cgtcgccggt gccggccccc tcgtcggccc ggtgctcgcg gcccagatgg
     6901 gctatctgcc cggcacgatg tggatcatca tcggcgtcgt cttcgccggt gccgtgcagg
     6961 actacatggt catgcacatg tcgatccgcc gccgcggccg ctcactcggc cagatggccc
     7021 gggacgagtt gggcccggtc ggcggctggg cggccattct cggcgtgctg acgatcatga
     7081 tcatcctcat cgctgtgctc ggcatcgtcg tcatcggcgc cctggccgaa tccccctggg
     7141 gcgtcttctc cgtcggcctg accatcccga tcgccctgtt catgggcgtc tatctgcgct
     7201 acatccgccc gggcgcggtc aacgaggtct cgctcatcgg cgtcgccctg ctcatcctcg
     7261 ccatcctcgc cggtgggtgg gtgcacgagc acgacacgct ctcgcagacg ttcaccctga
     7321 gcaaggaaac cctggcgtgg ttgctcatcg cctacggctt ctgcgccgcg gtgctgccgg
     7381 tatggctgtt gctcgccccg cgtgactacc tctccacctt catgaaggtc ggcacgatcg
     7441 cgttgctggc gctgggcatt ctcatcgctc ggcccgtcgt gaagatgccg gccgtaaccg
     7501 agttcgcgag caccggcacc gggccggcct tcgccggctc gttgttcccc ttcttgttca
     7561 tcacgatcgc ctgcggtgcg ctctccggct tccacgccct gatctcttct ggcacaacgc
     7621 caaagctgat cgagaaggag agccaagccc ggatgatcgg ctacggctcg atgctgaccg
     7681 aatccttcgt ggccgtcatg gcgatggtcg cggccgtgtc gatcgaccag cacatctact
     7741 tcgccatgaa cgctcccgcc ggcctgaccg gcggcacccc ggagaccgcc gcggcataca
     7801 ccaacgggct cgggctcgtc ggacccggcg gcgccgcgct ggcgaacatc gaccccgccc
     7861 tgctctcgca ggcagccacg gatgtcggtg agaagtcgat catcagccgc accggcggcg
     7921 caccgacgct ggcgttcggc atgtcggagg tcatgtcggc cttcggtgga gccgggatga
     7981 aggcgttctg gtaccacttc gccgtcatgt tcgaggcgct gttcatcctc accaccgtcg
     8041 acgccggcac ccgggtcgcc cgcttcatgt tctccgacgc gatcggcaac ctgcccggcc
     8101 tgggcaagtt caaggacccg tcgtggcgcg tcggcgcgtg gatctgctcg ctgatcgtcg
     8161 tcgccggctg gggctccatc ctgctgatgg gcgtgcgtga cccgctgggc ggcatctaca
     8221 ccctcttccc gctcttcggc atcgccaacc agttgctggc ggccatcgcc ctggccgtgg
     8281 tgacgaccat tctgatcaag cagggcaagg cgaaatgggc gtggatcccc ggcataccgc
     8341 tggcctggga cctgctggtg acgctcaccg cgagctatca gaagatcttc agcgacgtcc
     8401 ccgccatcgg ctacttcgcc aaccacgaca agttcgtcgc ggcgaaggaa ggcggaaaga
     8461 tcctcgcccc cgccaagacg atggaggaca tggacaaggt catccgcaac acgacgatcc
     8521 agggctggct gtcgatcatc tacgccgtcc tggtgatcat cgtcgttctc gcggccctcg
     8581 ccgtcatgct gaaagcgcta cgttccggcg gcctgccgac cagcgaggcc gaggacgtgc
     8641 cgtccaagat cttcgcgcca cgcctgttcc tgccgaccaa agcggagcag gatgtcctcg
     8701 acgaatggaa ggaagccggc ctggatccga caccggtcgg cgctcatcac ggatgacatt
     8761 gctcgacatg agtcgttcgc tccggtggtt ctggtcagga gtcaccggag cggacgccta
     8821 cgaccgctac tgcgctcacc tgcagcgcgt ccaccccgga tgtccactgc cgacgaagtc
     8881 ggacttctgg cgggagaagt atgccgagat ggagcgcaac cccaagagcc ggtgctgctg
     8941 agccttctcc ccgcgacacg ccacatgacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn tcgcgcgcgt cgacgaagga acaccggccg tccccggacg cgtgcaggat
     9121 gcggcgtcga tccagtcgcg cgcggacaac aacacgattc gctgcacccc ccgggcgttg
     9181 ctgaggtcca ggaaccgccc cagccggtcg accccgtgcg ggctttcgtc cacgatcaca
     9241 tagatcgcgt tcgcgccctc gagcaccgcg ggccaggggg cttcgtggct ccagtcgaac
     9301 ggcacggcgg tagaacgtga cactgcgcgc actccacacc ccgaactcgc aggttggccg
     9361 cgacacggct gccggtcgtc cccgagccgc agatgacttc gtcgaagatc aacacgatcc
     9421 cttcgcgcgt gccgaacgtg cgactcggtc acggcgtttg agatcagcag ccggatcgcc
     9481 tgcagatcgt ccgtgtccat cccgtgtccc aggagcacga tcacctcggg gtcgaaagtg
     9541 caacacacga taagtgcggc cttcgccgcc aactcccgga agtcgtgccg gtcctggagc
     9601 gcgttcagct gcgcctggcg gtcgatgccg aacccttcgg cgatgcgata gagctccccg
     9661 gcgaagccgg agtggccctg gaggaccgcg ccgtcgatca cgaagccgca cccgacatag
     9721 ccgtcctcgg ggaacagcat ggccgcgaga cttccgccgt tgtgcggggt ggaggtatag
     9781 acgcccgtgg cgatgaagtc catgtcattg cggacctccg ggttgatgcc gaacctatcg
     9841 cgcactcccc cggccacgtc cacgccgacc agagcatcga catcgcagtc ttcgacgaca
     9901 ccgtcacgtg ccactccggg gatgccgagg gcgatgccgc ggatcagttc gtcggtcgcc
     9961 agttgctccg cgatcagctc ctcgaggacg gaataggtga tggcgtccgg atgacgttgt
    10021 tcgctcagac gaacctgccc gagggcgtcc gccaccgcga acgccacggt gttctcaccg
    10081 tgctcggtga cgacatagac gaccaggaca tgaaggtagt ccgcgttgta ttcgaagcgg
    10141 cttgccggac ggccgacata gccgtcctcc tgggacgtgc ggatgatctc gccgtcggcg
    10201 cgcatctcgt tcaggatggt gttgcacgtc gagacgctca ggcccgtcca cgccgccatc
    10261 tcggccctgg tgcatgtctt gcgaacgtgc aactctctgc ggatccgctc gcgattgagc
    10321 tgccgcactt cgggcgtgct gctcaccatg tgcggcctct ccccgtcgac gttggccgga
    10381 tgctaccaat cagccgcatt tcacggccga tccgtgcccg gatcatcagc gtcggatgag
    10441 aagtcgcacg gggtagcggt cgccgacggt tgcctcgacc acgcccgggt tcccgggcgt
    10501 cagggcgtcg agatcgccgg acatctccag aatgctcgtc agggtctcgc ccttgatcag
    10561 gtcgcggtgt tcctccatcg cgcgattgac gaagtccccg cccgtcacga tcagcgcgat
    10621 gcggtcggag acgtccaggc cggcatcgcg ccgggcgccc tggacggctc ggattaggtc
    10681 ccgggccacg ccctcgagct ccaattccag tgtgacgtcg gtgtcgagga cgacgaagcc
    10741 gccgccggga agcaaacccg ttgcaacgct gccgcccccg tcggtgtcga caacggtctc
    10801 gagggcgtac tccccctcga ccagcggcag cccgcccgcg atgaccgtgc cgtcgtcgcc
    10861 gatggaccag tcgccgctct tgctgccctt gatggccacc tgcacgtcct tgccgagccg
    10921 cgggccggcg gcacgggcgt tgacggtcag cttctggctg atgccgaagt cggactggtg
    10981 tgcgtcggcg acatcgcgaa cgtctaccga cttgacattg agttcgtcgg cgatgacagc
    11041 cgagaagggc cgcagcgcag cggcatccgc ggtcacgacg gtgagtgatg acagaggcag
    11101 ccgggtgcgc agccgctcgg ccttgcgcag cgacgagccc accgagcaca cggtgtgaac
    11161 ccggtccatg gccgcgacga gggtgtggtc ctcgggcagg tcatagatgt tgggccagtc
    11221 ggtcaggtgc accgagcgcc cgcccgtcaa cccgcgccag atctcctcgg tcacgagcgg
    11281 cagcaggggg gcggcgacgc ggcataccac ctccagcacc gtgtagaggg tgtcaaaggc
    11341 gtccttgctc gactgcgtca ccgtgccacc ggcggcccag aaccggtcgc gcgagcggcg
    11401 gatgtaccag ttgctgagca cgtcgaggaa ggaccgcgtc agttcgcacg cgcgcgggat
    11461 gtcgtagacg tcctgtgccg caccggaatc cgcgacatac tcccgcagct tcgcgaggat
    11521 gtagcggtcg aggccgtcgg tggaatcggt ccgccactgg gcgtcatagc cggcggcccc
    11581 ggcgtagagc gcgaagaagt accacgcatt ccacagcggg atcagcgcct gccggacccc
    11641 ggcgcggatg ccctcctcgg tgacgatgag gttgccgccg cgcaggatcg ggctcgccat
    11701 gaggaaccag cgcatggcgt cggcgccgtc gcggtcgaag acctcgctga catccggata
    11761 gttgcgcagc gacttgctca tcttctggcc gtcgtcgccg aggacgatcc cgtgactgat
    11821 gcacgactgg aatgccggcc ggtcgaagag cgccgtcgcg aggatgtgca aggtgtagaa
    11881 ccagccgcgg gtctgcccga tgtactcgac gatgaagtca cccgggaagt gatgctcgaa
    11941 ccactcccgg ttctcgaacg gatagtgcac ctgggcgaaa ctcatggagc cggagtcgaa
    12001 ccagacatcc agcacgtcct cgacgcgccg catcgtcgac tgtccggtcg ggtcgtccgg
    12061 gttgggccgg gtcagctcgt cgatgaacgg ccggtgcaga tccggctcgc ccttctcgtt
    12121 gcgcgggagc accccgaagt ccgcttcgag ctcggcgaag gagccataga cgtcaagccg
    12181 cggatactcg gggttgtccg atcgccacac gggaatcggg ctgccccaga agcgattacg
    12241 cgagatcgac cagtctcggg cattctcgag ccacttgccg aactggccgt ccttgacgtt
    12301 gtcgggggtc cagtcgatct cctggttcag ctccatcatc cgctgcttga acttcgtcac
    12361 ctcgacgaac caggacgaga cagccatata gagcagcggc tgcttgcagc gccagcagtg
    12421 cggataggag tgttcgtagg tctccttgcg cagcagcacg gtgccctcgg tgacgggacc
    12481 cggatcggcc tgtcccccag cgccatccac gaacgtgcga gccttgaggt gttcggtgat
    12541 gagcgggttc gcgtcgaaga cgtgcacgcc ttcgtaggcg gtgaccggga agatgaaggt
    12601 gccgtccggt cccaccgtca gcaccgtctc gacgccgacc ttgtccagcg ctgccttgtc
    12661 ctcctcgccg tagccgggcg acatgtgcac gatacccgtg ccgtcctcgg tcgtgacgaa
    12721 gtcctccccc agcacctggt gggcgcccgc gtagccgacg tagtagggaa acggcggggt
    12781 gtaggtcgag ccgaccaggt cagaaccctt gaggcgagcc acgatccggc tgctgggatc
    12841 cttgcgcgtc gccacagcgt cctcggcctt gccgggccac agatcgttgg cgtaagaggc
    12901 gaggcgggcg gcggccagca catagcgctc ggtgcggccg gtcgcctccg actcgacgac
    12961 gacatagtcg acgtcgggcc cgacggcgat gcccatattc gacgggagcg tccacggggt
    13021 cgtcgtccag atcagggcga gggcgccggc atacggccca ctggccaggc gatagccgac
    13081 ggtgaccgcc gggtccttgc ggatcttgta gacctcgtcg tccatgcgca attcgtggtt
    13141 ggacagcgga gtctggtcgt tccagcagta gggcaggatg cggaagccct cgtagacata
    13201 tcccttgtcg tacaacgact tgaaagccca cagcaccgac tccatgaagg tcgggttgag
    13261 cgtcttgtag tcgttgtcga agtcgaccca gcgggcctgg cgggtgacat agtcctgcca
    13321 ctcgtgggtg tatttcatga ccgattcgcg gcacttggcg ttgaaggcct cgatgccgag
    13381 gtcgaggatc tcgtccttgg tcttgatgcc gagctggctc atcgcctcga gctcggcggg
    13441 caggccgtgg gtgtcccagc cgaagcggcg ctcgacgcgc atcccgcgca tggtcttgta
    13501 gcgcggcacg atgtccttga catagccggt gagcagatgg ccgtagtgcg gcaggccgtt
    13561 ggcgaagggc gggccgtcgt agaagacgaa ctcgttgtcg ccgttctcgc cggccgggcg
    13621 gttctcgatg gaggcctgga aggtcccgtc cgcgtcccag tacttcagca cggcttcctc
    13681 gatcgccggg aagcgggggc tggcggggac ggcgccggcg ccggtggtgc tcgccttggg
    13741 ataggccatc ggtatgcggt gctccatgct cggccggccc tcgtgaggcc ggttgtggtc
    13801 cggtcacgag gacgacgccg gccccggtcg gggccgccac cgcggtacca cctcgcttgc
    13861 cgcaggtcgc gcaccctcgc ggggacacgg catacgaccg ctcgtgccgg ctgtgacggg
    13921 ccactcccgt ccgggtctag tgagcaagag ggcggtatgc cgccgcctcg ccgttcttcc
    13981 ggaggctcgc cgctgatgac ggctcgaacg cctgtggtgc cgagtttacc tgccggcccg
    14041 ggtgggtccg cacgcgtatt tgcgggacgt tttccgtccg ggattgcccc tagcgtgggg
    14101 cgctatgggt gacttgacga tccggccggc taacgagaca actccagggg acatccaggt
    14161 cgtgttcggg accagaggtt cggcatgcga ttgccagtgc cagcgttaca aactcgcccg
    14221 cggcgaggcg ttcggcaagt tccccgtcga ggaacggcgc cggcggctgg aggaacagac
    14281 gggggcgggc gacccggacg ccgaggcgac gagcgggctg ctcgcgtatc tcgaccgaga
    14341 gcccgtgggc tggtgcgccg tcgagccgcg agcgaactac cacgggatgg cacgcaatca
    14401 gaaggtgccg tgggccgggc gcacggaaga caagagcgac ccgagcatct gggccatcac
    14461 gtgtgtcttc acgcgaaccg gcttccgcgg caagggaatc gcccacgcgt tggtagcggc
    14521 cgcggtcgaa catgcccgag cgggtggcgc ggcggcggtc gaggcctatc ccatcgtgac
    14581 gacaccaggc gcacggatca cgtgggacga gatccacccc ggcaccccgc agatgtacgc
    14641 cgcggcgggc ctggtggagg tggcgcaccc gtttccccgc cgcctggtca tgcgccgaga
    14701 cttcccctag ccggcgggag gaggcgagtg ggcggcatac cgctgctcca agacccgctg
    14761 gtcggttccg aactcggcga tcgggcaccc caatccgttg ggtttgatcg cgatcccctc
    14821 ctcgcgccag tgcgcgaagg cctcgtcgcg cagatgcatg ggcgggtccc cgtaggcgtt
    14881 ggtgacgcgc caccaggcga ccccaccgcc ataccggctc atgatcgccc cgacctggcg
    14941 gggaccgacg ccggcgatct gaccgacctc gccgtatgcc gcgatctcgc ctcgggggat
    15001 cagctcgacg acgcgcagga cacgctcgac cgcggcctcg tccatgccgc gcgacattaa
    15061 cggacgcggt tagcattgcg cgaccggccc taaggagatt ggcgtgcgtg tagcggtgct
    15121 cggtgcgggc tcctggggaa cggccctggg ttatgccctg gcgaccaagg gctacgacgt
    15181 ggccatgtgg gacatcgata tgcctgtgct gcagtcgatt acggcgcacg agaacacccg
    15241 ctacctgccc ggcatcaccc tgccgtcatc catgcacgga gagcccgaac tcaaggcggc
    15301 gctcgcccgc gcccagttgg tggtgttcgc cgtgccctcc caggcgatgc gctccgtggg
    15361 cacgcaggcg gccccgctga tccgggacaa ggcactgttg tgctcggtga ccaagggcat
    15421 cgaggtcgac accctgatga cgatgagcga ggtactccag gacgtcctgc cgcagcgcct
    15481 gcactcgcgg ctgacgttcc tatcggggcc gtccttcgcg gtggaggtgg cgcgggagtt
    15541 cccgacggcg gtcaccatcg ccggcgagga cgctgaggtg acctcccagg tgcagcgcgc
    15601 cttccacacc cccttcctac ggccctacgt gtccgacgat gtcgtcggcg tcgagatcgc
    15661 cggctgtgcc aagaacgtca tcgcgatcgc ctgcggcgcc ggcgacggat tggggtatga
    15721 cgccaacacc cgggccgcga cgatcacccg ggggctcgcg gagatcaccc ggctcgccgt
    15781 ggcccggggg gcgaacccgc tcaccttgct agggctggcg ggggtcggcg atctcgtgct
    15841 gacctgcagc tcggtcaagt cgcgcaacta ccgggtgggt ttcgggctcg gtcagggccg
    15901 ccggctcgac gaggtgcagg ccgagctcgg gcaggtggcc gaaggcgtgc tgaacgccaa
    15961 gtcggtcaag gcgctcgccg agcgggccgg ggtggagatg ccgctgtgcg gcatggtgta
    16021 tgccgtgctc tacgaaggcc gggacatggc cgatctgcgc tccttcttcg ccgagcgccc
    16081 gctcaagtcc gagccggagc accacttcgg ccgctggaca cgtcgcttcc gccgccgtgg
    16141 ccgccgcgtg gccgccggcc cggccagccc agatgcatga cgtggcctgt ggacagacag
    16201 cgctgacgtc ctcacggtcg catccctgtc ggatcgcccg tgtgacctcc agcaccgtgc
    16261 caggcgcggg cctcgtcgtc gaggcccgcg ccggaggcta gactcgactc cggtgccgct
    16321 ttcgcggtgc cccgcacgtg cgaggggaag ccggtcgaag tccggcgctg acccgcagcc
    16381 gtaggccgcc cacaccgggt ggcgagccgg aatgcctcgt ccgctgcggt cgtcctgtcc
    16441 accgccgagg catgcggtac gcagggccgg gcaggaccgg ctcacgtatc gcgcgaggag
    16501 ccggagtcat cgtgcatcgc cctcccctga ctgttgccgc gtccgcggtg gcgctggcgt
    16561 gtgccctgac gatcggggcg gcgccgggag ctgtggcgga tccgagcgcc ggcgcatccg
    16621 acggggcagc gttcctggcg cgcgaactgg ccgcgtccgg tgacgtgctc gactaccccg
    16681 gcaccgacca ggacgacatc ggcgtcacga tcgacgcgct gttggggatg ctcgtcgtcc
    16741 gctccggcaa gacgcaggtg gagcgctcga gcgacgccgt cgcggcgaac ctcgacgact
    16801 acctcggtcc cacattcggc gccaccgagc tgtatgccgg gccggtcgcc aagtcgatgc
    16861 tcctcgccgc cgcgacgggt cgcgacgtgc acgcgttcgg cgggcacgat ctcgtcgcgg
    16921 acctgggtgg gctggaagcg gccgacggac ggtatgccga ccggacggca tacggcgaca
    16981 cgagcaacac cttcactcag gccttcggtg tgctggcgat gactcgcgcc ggcggcgggg
    17041 catccgagaa ggcagtgaac ttccttgtgg cccaacagtg tccggacggc ggcttgcgga
    17101 tggtgcccgg cactgacccc tgcgtcagcg atccggatgc cacctcgctc gccgtggcgg
    17161 ccctgctcgg agccggtggc cgcgaggccg aggcggcatc cgcgctcgac tacctctccg
    17221 gcgcacagac ctcggccggc ggtctcggcg gcggcacggg cgccacgggg gtcaacagca
    17281 acagcacggg cctggccggt atggccttcc ggctcggggg gcgcgaggcg gaatgggcgc
    17341 tggcgaacga ctacctcggt ggtctccaac tcggctgtga cgcaccggaa tccgtgcggg
    17401 gggccgtggc ctacgaccag gccgggtatg acgcgctcgt ggccgagggc ggcggggctg
    17461 tggccggcga tcaggagcgc cgggcgaccg ctcaggcggt cctgagcttc ggccccgacg
    17521 ggctgctgga cgcctccgtc gatctggcga ccgacgccgc cggcgactgc tcagactcac
    17581 cctcgtcgtc gagcacgtcc agcacgtcga gcacgtccag cccgccgacg agcggcacac
    17641 cctcgcagac cacgacgccg accacgacca cgcctgcgcc gactgccgcc accgtgaccg
    17701 cgacggtcac ggtcaccaca accgcgtcga ggccggcgcc gcccgctccc ccggcgaccc
    17761 ggacggtcac cgtcactccg gaccacgaac gcgccccgac tcgtgccgcg acgtcgtcgt
    17821 cctcgcctgc ggcgtccctc tccagcaccg aggcggccac ctcgccgacg ggcaccagca
    17881 cgccaccccc ggcatcagcc actgcgtcac cggccgcccc cagcgccgac cccagggccg
    17941 ccgtcacggc ggcggccacg agcggggctg ccacaaccag ttccaccggg ccggccatcg
    18001 cggctgccgg tggcctcgtc ctggccggcg ccatcggcgc ggccgctgtc ctccgtcgcg
    18061 gaaggaagtc cccatgaaat cccgcctcct cacccctctc gctgctctta cggccgccgc
    18121 accgctggcg ttcggcaccg ccccgtcagc gcacgcgacg tccggcacgg cgctcgcgat
    18181 ctgcccgcgc ggatcgggcg tgacggtcat cgtcgacttc ggctcatggg acaagaggtc
    18241 ctgtgccccc ggcgacccca cgaccggact cgcggccttg cggggtgccg ggttcacggt
    18301 gacgatggtc cagcgcttcc ccggggccgt gtgccggatc aacggccgtc cctcggccac
    18361 cgtcgacaag tgcgtcgtca tgccgcccac cacggggtac tggtcctact ggcacgccgt
    18421 ccggggcggc aagtggacct actccacgtc cggccccggg agctacaacc ccaagcccgg
    18481 caccgtcgag ggttggtcct atggcgccgg caagcccccc accgctcccc ctcccgccta
    18541 ggacgtcatg acgcagctgc gccacgctcg gctcggtccg cccctcgcta tcgccgcgct
    18601 cctcgcggcc ggtggcgtca tcgccacccc ggccgccgct gccacctgtt cctcgaccga
    18661 cggggtgagc gtcgtcgtcg actcgggcaa cggcatacaa accggttgtg ccccagggga
    18721 tcccgcgtcg gcctgggcgg ctttggagtc cgccggtttc cggctggaag gcacgcagcg
    18781 attcccggcg agcttcatct gtcgcatcaa cggctatccg gcgccggccg atgagccctg
    18841 tgtccagact cccccggcca cggcgtattg ggcgctgtgg agtgccgcgg ccgggggtgg
    18901 atggacctac agcagcaccg gcgcggccag cctgaacccg gcaccgggca gctccgtggg
    18961 ggtcgccttc ggcgccggcc ggcagccggg catggcgcca ccagtcgtgt cgccgccgag
    19021 caccacggcg cctgccacga catccacgac cagagcaccg acctcatccg gcacgacatc
    19081 ggcgcactcc gaaaccgctg gggcgcaaca cacctcggct gccgacccag gctcgtccac
    19141 gccaggctcc tccacgggcg cggcgggccc cgcgggcaca cgcaccacct cctcggcgtc
    19201 agcgaccacc tcgcggccgt ccacctcggc cacgtcgacg agtggcagcc ggtcgacgag
    19261 ttcatccagt tcggccaccg tccccaccgt caccgccacg accgagccac cgagcgcggc
    19321 agcgagtccg caagccggcg aatcccccag cccggcgctc atcgtcgccg gcggggccgc
    19381 gctcggcctg ctcctcgtgc tcggcgcctt tgtcgttcgc cggcaacgcg atcgagccgc
    19441 ggcgttgtga acctcggttt cgtgaacctc tcgtgatccg tcacctgcac cccgcggcct
    19501 ggtggctgtg ggcgctggcc ctggcggccg gcgcgagcat gacgacgaat cccgtcctcc
    19561 tcgccgccca tgtcggagtc ctcgtggccg tcgtgctggc ccgtcgggac ggatcgccgt
    19621 ggggacgcgc cttccggctg tatgccgtgc tcggcgtcgt catcgttctc gtccgcctcg
    19681 tcctgcacgc cctggtcggg ctgaagttcg gcgacatccc gctccttccg ctgcccgagg
    19741 cgcacctgcc ctcctgggcg gccggcatcc aactcggagg aaccgtctat ctggaagggg
    19801 ccctcggcgc gggtctggag ggggctcgtc tcgcgacgat gatcctggcc atcggcgcgg
    19861 ccaacgcgct cgccagtccg aagcggctgc tcaaggtctt ccccgcggcg ctgcacgaga
    19921 tcggggccgc gttggtcgtg gcggtcacgg tcgcgccaca actggccggg agcgtgcaac
    19981 gtgtcgcgcg cgcccggcag ttgcgcggcg aacccgcctt gcggctcaag gggtttcgca
    20041 gcatcgccat gccggtcctg caggatacgc tcgatcgctc gctggcactg gccgcagcca
    20101 tggacggccg tggttacggg cggcgaccgg agtccacgaa tggccgggca accggtgcct
    20161 tgacgctgct cggactgctc ggctcgtgcg tcggcatcta cggactcctc gacaccacat
    20221 cccccgccgc gttgggcttg ccgaccctgg tcgtcgggtt ggtgctctgc gttgccggcc
    20281 tctggagcgg cggacggtcc gtgccgcgaa cgatctatcg ccgcgatccg tggagtgcac
    20341 cggagtggtt gacgatcgcc tgcggcgccg cggcgctggt cgcgatgctc gtgaccgtcc
    20401 gccgtgaccc ggctgcgctg ggtatgccgc tcgctcccct cggcctgccc gccctccccg
    20461 tgctgccgct ggccggtctc gctctcgccg cggtgcccgc ggtcgccact cccgtgccac
    20521 cccttgtgtc cgcccgccgg gcacctcggg aggtggcggc gtgacggcat acgacccggc
    20581 gatctcgttg tcccatgtga cgatccacta tcccgacgcc gccgaggcgg ctctgcgcga
    20641 tgtcgacctc gagatcgagg agggcgaact cgtgctcgtc atcgggcgca ccggttcggg
    20701 caagtcgacc ctcctcggca ccctcaacgg ccacgtcccc cacttcaccg gcggacggct
    20761 gtctgggagg gtgcgcgtgc tcgggcgcga caccgcgacc caccgcccgc gcgatctcgc
    20821 cgacgtcatc ggtgtcgtcg ggcaggaccc actcgccggt ttcgtcaccg acaccgtcga
    20881 ggaggaactg gcctacggca tggagcaact cgccctgccg ccggccacga tgcgcaaacg
    20941 ggtcgaagag accctcgacc tgctgggcat cgccgacctg cgcgccgctc ccctgcgcga
    21001 tctgtccggc ggtcagcagc agcgcgtggc gatcggctcc gtcctggccg cgcacccgcg
    21061 catcctcgtc ctcgacgagc ccacgtcagc gctggatccc actggggcag aagaggttct
    21121 ggccactctg acccggctgg tccacgacct cggcgtcacc gtcgtcgttg ccgaacaccg
    21181 gctggagcgc gtggtgcagt atgccgaccg cgtcgtcgag gtcgttgacg gcacggtgcg
    21241 cgacggcata ccgtcggcga tcttcgagac ggcctcggtc gccccgcccg tcctcgatct
    21301 gggccggtat gccggctggt cccccctccc cgtcaccatc cgcgacgccc gccgcgccgc
    21361 cgcccccctc cgcgcccggc tgggtgatca aggaacctct gctagcagag gctggtcaaa
    21421 tcagcagacc tccctaggtg tgcagatttc actaacctct gctagcagag gttcctcggc
    21481 ttccccgggg gtgcgggcgc ggggggtgcg cgtcgactac gggggcgtgc gggcggtggc
    21541 ggacgtcgac ctcgacctgc cggccgggcg ggtgaccgcg ctgatggggc gcaacggggc
    21601 gggcaagtcg tcactcctgt gggcgttgca cggcgcgggt acgcgcacgg ccggccgggt
    21661 cagcctcgcc gacggccggg atccggccgg cctcaagccg ggcgtacgac ggggcgccat
    21721 cggcctggtc ccgcagacgg cgaccgacct tctctacctg gataccgttg ccgcagaatg
    21781 ccgtcaggcc gacaccgagt ccagccgaga ctcgggcaca tgcgcgcgcc tgctcgagga
    21841 actggctccc ggcatacccg gcgatcgcca cccgcgcgac ctgtccgagg ggcagaagct
    21901 cgccctggtc cttgccgtcc aactcaccgc cggcccccag gtgctgctgc tcgacgagcc
    21961 gacgcgcggg ctcgactacc ccgccaaggc ccgcctcgcc gacaccctgc gcgggctcgc
    22021 tgatcacggg cacaccgtcg tcgtctccac gcacgatgtc gagttcgtcg ccgagagcgc
    22081 cgacgaggtc gtggtcatgg ccgaaggcga ggtcgtcgcc cagggcccga ccgccgaaat
    22141 cgtcgccgcg tcaccggcat tcgcgccgca ggtcgccaag atcctggcgc ccgtcccgct
    22201 gctgacggtc gcgcaggtgc gcgccgcgct cgccgagcgg gccgcgccgc aggacacggt
    22261 atgacgcccg cctccgccgt ccgcctgcgg ccgcggtcgg tgctcgccat cgccgtggtg
    22321 tcggtcgcgg gactgctcgc cttcggctgg cctttgatca tcgaccccgg gtccgggatc
    22381 gcgcacggcg gggacgcgcc gttcgtcttc gcgctgctgc tgccgctctt gctcgccgtc
    22441 gtcctggcgg agatctccga gggcgggctc gacgtcaagg ccgtcgcgat gctcggcgtc
    22501 ctgtcggcgg tcggggcggt gctgcggccc ctcggggccg gcagcgccgg ggtcgaactc
    22561 gtcttcttcc tgctcgtcct cgcgggccgg gtcttcgggg ccggcttcgg cttcgtcctc
    22621 ggcgccacaa cgcttttcgc ctccgcactg atcaccggcg gcgtgggccc gtggctgccc
    22681 taccagatgc tcggcgccgc gtgggtgggg ctcggcgcag ggctcctccc ccgcgccacc
    22741 ggacgcgccg aggtggccct gctcgcggca tacggcgcgc tggccggact ggcatacggc
    22801 tggctgctca acttctcctt ctggcccttc gccgtcggcc ccggcaccgg cctgtcgttc
    22861 gttgccggcg acccggcgac ggccaacctg caccgcttcg tcctcttctc catcgccacg
    22921 agtcttggct gggatctcgg gcgcgccatc accaatgccg tgctcatcgc cgtgaccggg
    22981 cgagccgtcc tcggcgcctt gcgccgcgtg gcccacaagg cggcattcga tgcgacgccg
    23041 gagttttcga gggccccgga ggcctcggcg atggtagaaa cacgggatga gcccagctga
    23101 cgtcgagcgc caggaatcct ggctcagcca ggaggacctc gccacggtcc gtggccggat
    23161 gccgatcctc tatgtcgagg ccgttccggt gcgcgtcgcg gccgacgggt ccgtcaccgc
    23221 tgtcgggttg ctgctgcggg cctcggacaa cggtcggatc cagcgcgaac tcgtctcggg
    23281 ccgcgtgctg tttcacgagc ggatccgcga cgcgctgcta cggcacatcg aaaaggatct
    23341 cggcgcgatg gcacttcccc agatcccgcc cagccccgtg cccttcaccg tcgccgaata
    23401 cttccccact cccgggatca ccgccttcca cgacagccgc cagcacgcgg tcgcgctcgc
    23461 gttcatcgtg cccgtcgccg gcgactgcgc gccccagcag gacgccctcg acctcgcctg
    23521 gctgacgccg cagcaggcgg ccgacccggc gatcatcgcc gacatggacg gcggccacgg
    23581 cgtgctgctg cgccacgccc tcgcccacct cggccacgcc atctgagccg gcccgcgacg
    23641 gaaatgaagc gcgacgacgg gccgagacgt gcccagcctt gtggataact cgggccggac
    23701 cggacgagca tcggccattc tcaggagatg gcagagctcg aggaagtggg gcaccatgac
    23761 tgctatgacc acgttgccca ggagccggcc gttgacgcgg gcagacctgg ccgcgatgcc
    23821 cgaggacggg caccgctatg aactcatcga cggagtcctg atcgtgacac cgggccccgt
    23881 aacccatcac caacgagcat cccggcaact gctcctggcg ctcagccggg atgtccccgc
    23941 ggacctcgag gtctttcacg cgccgttcga cgtcgtcctc gccgacgaca cggtgctgca
    24001 gcccgacatc gtcgtcgcgc gccaggccga cctgaccgcc gccaatctgc cggcggcccc
    24061 ggtgctcgct atcgagatcc tctcgccgag cacgcgacac atcgacctgc acctcaagaa
    24121 gagccgtctc gaagaggccg gatgtgccgc ctattgggtc gtcgaccctg gcgagccgag
    24181 catcaccgcc tgggagttgc gtggtggtcg gtatgtcgag gtgggtcatg ccgtcggcac
    24241 cgaggtcctc tccgtccggc agccgtatgc cgtgaccatc cgcccggcag acctcgtcag
    24301 ctgacgccgt ggtccacgcc cagcagggcg cccgcgcatc gtcatccgtg acagtgccta
    24361 acagtcatag tccacggtga ccgcggcgtg gtcgctcatc cgtatgccgt cgacctcccg
    24421 gtccacccac gtccgccggg ccgtgcgcgc gaggccggga ctcgcgaagt ggaagtcgat
    24481 ccgccagccg gcgtccgcgg catacgtggt cccgagccaa ctccaccacg tgtacggccc
    24541 cgcaacatcg ggatgaagcc ggcgcaccac atcgacgaaa gtccttggcc cgagcaggtt
    24601 ttcgagccac gcccgctcgg gccaaggact ttcgtcgatg tggtgcgccg gcttcatc
//
LOCUS       DAOPJG010000020        24015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2228_length_24015_cov_7.582304, whole genome shotgun sequence.
ACCESSION   DAOPJG010000020 DAOPJG010000000
VERSION     DAOPJG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 24015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24015
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2228_length_24015_cov_7.582304"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PKU75_03580"
     CDS             complement(<1..770)
                     /locus_tag="PKU75_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPF79987.1"
                     /translation="MTTKVLAAVAWPYANGPRHLGHVAGFGVPSDVFARFMRMSGNEV
                     LMVSGSDEHGTPILVLADKQGVTAREFVDVNHAIIAHELADLGCSYDLYTRTTTANHY
                     AVAQELFSQVWKNGYMIERTQKGAISPSTGRTLPDRYIEGTCPICGYTEARGDQCDNC
                     GNQLEPEDLIDPKSKINGETPDFVETQHFFLDLPALAGALRTYLEEREASGTWRPNVI
                     KFSLNILDDIKPRAMTRDIDWGIPVPLHGWRDLPTKKL"
     gene            1104..1457
                     /locus_tag="PKU75_03585"
     CDS             1104..1457
                     /locus_tag="PKU75_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79988.1"
                     /translation="MTSIVGGLALAGSLGVGTATSANASTDSIRAAGVSVSAGDAKTA
                     VSCPVRYITRGIRVTCPASIPGYAFRAVVKCKRANGTTFLRKDAWRVQGTGYSQARCP
                     LGTKRVYLPYAFEYK"
     gene            complement(1552..2166)
                     /locus_tag="PKU75_03590"
     CDS             complement(1552..2166)
                     /locus_tag="PKU75_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPF79989.1"
                     /translation="MIAALTGIGLSAAAGLNAYVPFLIVALIARFTDVLTLPSSFSWM
                     ESWWAIGIGSLLLLTEVAVDKIPAVDTINDAVQSFIRPSMGGLVFAATSAAETIDKSQ
                     WMQDNQWVGIVLGIIVSGLVHSGKMVARPAINAGTLGAGAPVVSTMEDGASIGLSLIA
                     IFIPILVILALLLLFGMMFWVWRKVRKWRQRRRQDLDDAGVSYA"
     gene            complement(2276..2890)
                     /locus_tag="PKU75_03595"
     CDS             complement(2276..2890)
                     /locus_tag="PKU75_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79990.1"
                     /translation="MPRDVAPAGLPAPVTGRRKDLLDAAAAVVARSGLRGLTHRAVDA
                     EAGVPEGSTSGYYRTRLALLSALTAHVSGQLAAAVDAARLRIAALDLADPGQDERIED
                     EIVALVVGQAARADLVRVQVELGVESSRSPQLGEFFDSWRTKLVGLVMGIACEVAPDH
                     ANERAEITVAAFQGVLMSSLLRPEGERAAYVEGATRQLIALLSA"
     gene            2982..3173
                     /locus_tag="PKU75_03600"
     CDS             2982..3173
                     /locus_tag="PKU75_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF79991.1"
                     /translation="MSMILSHAAAVATFLTVVLAIAGALVALALLAAAGDFLARNHTM
                     RVRRHQSVPVYYRHLVTGH"
     gene            3253..4047
                     /locus_tag="PKU75_03605"
     CDS             3253..4047
                     /locus_tag="PKU75_03605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPF79992.1"
                     /translation="MPSLTAAEPGPLPWTGLSRRHLVLETWLVLGVSLGASAIWSLLR
                     IIDRLTVHVALNQQTTSMNNSVTPDRPWLDLAYQLTGVILGVVPALLALHLLRRDDAG
                     AWRAIGWDRQAPGRDLLAGVGLTALIGIPGLGLYAAARALNLNTTIAAANLTDHWWTI
                     PVLVLAAAQNAVLEEVVMVGYLLTRWRQAGWPAPLAIAISALVRGSYHLYQGFGGFAG
                     NLVMGLVFGYLYTRTRRVLPLVIAHTLLDVFAFVGYTLLKDHLSWL"
     gene            4088..5338
                     /locus_tag="PKU75_03610"
     CDS             4088..5338
                     /locus_tag="PKU75_03610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF79993.1"
                     /translation="MLRRLGFPDFSHHKALVTGVGIDALGSGVFMPVSILYFLMTTEV
                     GKAQVGLAMSIAAALAVPFVLAVGGLVDRHGAKTIILAGNVLQAVAFVGYLWAQDFWT
                     IVAVQALASLGQSAFWGAYSPLVAASTPPDQREVWFGFLGALRNVGFAIGGLASGIVV
                     TIGTSAAYHAVVLVNAFSYILAFAALAKVPGGRAASDGPQDADAPARGGWGVLARDGR
                     YLGLVGANAAYALCTVSLNIAIPVYAIDVLKLPGWVSGALFVVNTLMVGFGQGLIVRR
                     MDGHRRHQIVILGFVLYAAGFLALAASGTLPVALATAAIPVSVAIYTLGELLGGPPLS
                     AAAVDGAPERLRGRYLSAYQLSWVASGIIAPALYLALLAHDRFSVWATLIGIAALGCV
                     GLRALAPRMPAVSAVVTSAQVTHA"
     gene            complement(5357..6304)
                     /locus_tag="PKU75_03615"
     CDS             complement(5357..6304)
                     /locus_tag="PKU75_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF79994.1"
                     /translation="MATQIIETRDLVREYKKLRAVDHISFTVEPGEAVGYIGANGAGK
                     STTIKMLTGILRPTSGLVRTCGRDPLRERIALAREIGVVFGQRSQLWWDLPLQESFTI
                     LGAIHRLDPAVARVRSGELVDLLEMGGFLSTPVRQLSLGQRMRAEIAASLIHRPRLLI
                     LDEPTIGLDVLSKQRLRDFLGAERREHGTTLLLTTHDMGDIERLCDRVLVVDHGRLAY
                     DGTLSGLAKTVGAERVMVVDLAPQAGHEGVAELRDIPGAALVGVEGEGKRQRLAFDPQ
                     ATSAAAVLAAVSARADVLDLAIEEPDIEDVVRRIYAASR"
     gene            complement(6304..7140)
                     /locus_tag="PKU75_03620"
     CDS             complement(6304..7140)
                     /locus_tag="PKU75_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPF79995.1"
                     /translation="MAEPAGRPTLSVGERLRPYRVILGARMRAQRQYRASFVTDIVGT
                     LLVGVTEFAEVWGVFHNVPVFGGLTYGQAIVVFALSNLCFSLADMLVGHLDTLPQVIR
                     AGQVDAYYVRPLPVLAQFMTSDISLRRIGRLAVALVLLVVGLAMAQVAWSPAKGALLV
                     LSIVSGTVIFSALFTVAGGAQFFLINGPEFTNGFTYGGSYSAQQPQPIFPDPVRIFWT
                     FVIPTAFVAYLPTIVLLDLPGDGFVPRWLAWASPAAALASCALAGLVWRFGTRHYQGG
                     GG"
     gene            complement(7133..7939)
                     /locus_tag="PKU75_03625"
     CDS             complement(7133..7939)
                     /locus_tag="PKU75_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPF79996.1"
                     /translation="MTRVLRTTALLVRSGFRQQTTYRLALLAGISTNSVFGIIRAAIA
                     LATVASAGAAVGGYDARTAAAFVWWGQALLGTINLWGFSLVADRVRSGDIAVDFLRPV
                     NPMLVYLAGDVGRAGVGLVARAIPTLAIGALLFDLAWPPGMPSAALGLLSILLAVVVA
                     FLGAFSLNLSALWFTEIRGLRLAWMVVGGVLCGLYLPLPWFPGWVQAVASCTPFPSML
                     QHPLDILSGRIAGADAVSAVVSQLFWVLVLTVVGQWVLARGRRRVEVQGG"
     gene            complement(7986..8522)
                     /locus_tag="PKU75_03630"
     CDS             complement(7986..8522)
                     /locus_tag="PKU75_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HPF79997.1"
                     /translation="MTAEQPPASEGPIVDPDELAQFWEIAKVKLRLNPVATYTGETAA
                     STLIPPAWAFGATPEHADDLLALVLAGTKTGTAGALADYEADGDEPERPGDLAIILDG
                     RAHPRAVIETTAVSIVPFDQVDAEHARSEGEGDLTLESWREIHERFFTAYSSAGFTSD
                     MPVVCQRFRVLYPTARNA"
     gene            complement(8760..10721)
                     /locus_tag="PKU75_03635"
     CDS             complement(8760..10721)
                     /locus_tag="PKU75_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HPF79998.1"
                     /translation="MKKIPCVVSAAAFALVVAPTAGIAGTSSGDGRVTADEARSLNAG
                     VQVAPKSSSVRSGSAAATNPYLGMVLDQSSVDYSYWKSVLRKGAAERARSSRLEENKR
                     AASGGNLRAPAIYDEKEIDGTQSNDSRAEAERIGVFGTAAGKNNRVRILGSQQQYSLS
                     ETTLAPVPEDNGSIPLAGDSGLGPREKTTTTGTIGDGPHGSAGDGTGDVDFYSITAVA
                     GEQLILDTSGGTLDTLIGLYSADGTLLAVDDDAGSGLASLLTYDVTESGTYYAAVVGY
                     KSGGSFPADPMDSGSGNGVGREGDYPLAMTRTTVDKDFYAVWLRPGDVLGGSGLSGAD
                     SLTVFRPDGTEMVGAEEMDASFLYPPNSPLPGAGTTALAYVAEQAGWYALQVDGAQGD
                     YQVALEAYRPGGQLAPGPVQRIFLDFNGQRVNTAIWGGSGVRTLSPFNAFLSSWGLSK
                     SQERELINKITTEVNENLKWDLRAKGLNPNVQVTIVNSQNSADIYGKARVSRVIVGGT
                     IAESGISTIGIAQYIDPGNYAMDDQALVLLDAMSEPSGPAYSLNTYMNESSDRVAFVS
                     QAVGNVVAHEVGHLIGNYHTNNTNETANLMDAGGTGFVDLFGVGPDGVGGTADDTDTD
                     FGTDEYIPAEGFTGLENTLNVSAWAFTKR"
     gene            complement(10899..11642)
                     /locus_tag="PKU75_03640"
     CDS             complement(10899..11642)
                     /locus_tag="PKU75_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPF79999.1"
                     /translation="MNLTLKIWRQDGPTARGELVTYQVPDISPDMSFLEMLDVLNERL
                     NAEGKEPVAFDSDCREGICGMCGLMISGDAHGPEKTTTCQLHMRSFKDGETITIEPWR
                     ATAFPVLRDLVVDRTAFDRIIQAGGFISANTGAAPKAEAILVPKENADRAFMAAECIG
                     CGACVAACPNASGMLFMGAKVTHLGELPQGQPERAQRVAAMVATHDAEGFGGCTNIGE
                     CSHACPKGIPLDVISQLNADMRKAKSGRL"
     gene            complement(11653..13656)
                     /locus_tag="PKU75_03645"
     CDS             complement(11653..13656)
                     /locus_tag="PKU75_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPF80000.1"
                     /translation="MTETLNKPEGVDADYYSVGAPIADTKAPAGPIENKWTSRKFDAS
                     LVNPANRRKLSVIIVGTGLAGAAAGATLGEAGYHVKSFCFQDSPRRAHSIAAQGGINA
                     AKNYKEDGDSIYRLFYDTVKGGDYRARETNVYRLAEVSANIIDQCVAQGVPFAREYGG
                     LLDNRSFGGVQVSRTFYARGQTGQQLLIGAYQALERQIAAGTVEMYARHEMLELIVAD
                     GQARGIIARNLVTGEIETHLADAVVLASGGYGNVFFLSTNAMGSNVTANWRAHRKGAY
                     FGNPCYTQIHPTCIPVSGDHQSKLTLMSESLRNDGRIWVPKRKEDCEKDPRQIPEEDR
                     DYYLERIYPAFGNLVPRDIASRQAKNMCDEGRGVGPMVGDFRRGVYLDFADAIARLGK
                     PAVEAKYGNLFDMYARITGENPYETPMRIYPAVHYTMGGLWVDYDLQSSIPGLFVTGE
                     ANFSDHGANRLGASALMQGLADGYFVLPNTIRDYLAKGPYPTLDENDPAVAEARTSVE
                     DRTRRLLAINGERSVDSFHKELGHIMWEYCGMERTETGLMKAIDLIRSLRAEFWRNVR
                     VLGAADTLNQSLEKAGRVADFLELGELMCIDALHRRESCGGHFRAESQTEDGEALRHD
                     DEFLYVAAWEYAGGSGEGEMGAPVLHKEDLAYEFIQLKQRSYK"
     gene            complement(13661..14356)
                     /locus_tag="PKU75_03650"
     CDS             complement(13661..14356)
                     /locus_tag="PKU75_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPF80001.1"
                     /translation="MATSTLPASTPAVRSSIGLKLLMAGSGLLFVFYVLAHAYGNLMV
                     LGGPDAFNNYAHHLRTLGEPMLPYSGFLWVFRLVLIAALALHVYSALTLWGRANGARS
                     TKYAVKKAAASSLASRTMRFGGIALLLFVIFHLLHLTTHTIRPQGEQPTPYANVINSF
                     DKWWLVLIYAVAVIALAFHLHHGIWSAAQTLGWTSTTAARRNAKFLAHFIGSLVATAF
                     LIPPLCILFGIVD"
     gene            complement(14506..15762)
                     /locus_tag="PKU75_03655"
     CDS             complement(14506..15762)
                     /locus_tag="PKU75_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF80002.1"
                     /translation="MSAPANDPTLALPADRQDERFIADEGLSGWLKGSLARLRSGDLG
                     SMPVVVGLIIIWGVFYALNPAFLSSRNLVNLTLQMVPTGTIAIGIVLVLLLGEIDLSV
                     GSVSGLAGAVLAVGFVHHGWTSGLAIGVALLVGVVIGVLYGLLFNRYGVPSFVITLAG
                     LLAFLGLQIKVLGKDGTINLSFNSDIVQFASQKFVPDVVAYLLVLLAVGASLAAKMAQ
                     GRKRVAAGLSATPTSVVLARAGILAVILLAAVYILGRDRGVSYMVLVFIALVVVMDLV
                     IRRTRWGRSVMAVGGNVEAARRAGINVKRIYLTVFALCSVFAALGGVFAAARLASVSQ
                     SSGGADTNLNAIAAAVIGGTSLFGGRGSAYSALLGILVIQSISNGLGLLSLDTSYKFM
                     VTGGVLLLAVIIDSLSRRSRAAHGRA"
     gene            complement(15759..16580)
                     /locus_tag="PKU75_03660"
     CDS             complement(15759..16580)
                     /locus_tag="PKU75_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF80003.1"
                     /translation="MTTTSPAAPVLSMRGISKRFGAVQALKDIEFDVSPGEVVALVGD
                     NGAGKSTLVKAIAGVYQPDEGEVTFDGSKVDITSPAEAQRLGIATVFQDLALCDNLDV
                     VANLFLGSEVTGAGMLDEVAMEKESWRLLRELSAKIPSVRIPVASLSGGQRQTVAIAR
                     SLLGKPKVVMLDEPTAALGVAQTAEVLNLVERLRENGLGVILISHNMSDVMAVADRVV
                     VLRLGRNNGEFRVADTTSQEIIAAITGATNNAVSERAARRDPDATPTQDTIEEQL"
     gene            complement(16610..17761)
                     /locus_tag="PKU75_03665"
     CDS             complement(16610..17761)
                     /locus_tag="PKU75_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPF80004.1"
                     /translation="MHTLRAPRAASLAIVAAMSVTAIAACGTNSSTTSSTSSGSAAAT
                     SGSGSASASASESASSAGAGGGKIALLLPESKTTRYETFDRPLFEAAVKAACADCEIL
                     YSNADQDAAKQQQQAEAALTQGAKVLVLDAVDGKSAQTIVANAKGQGVKVIAYDRFVA
                     GSDYYVSFDNKKVGELQGTGLVDALKAAGKTSGDIVMINGSPTDANAADFKAGAHSVL
                     DKSGFTIKAEYDTPDWSPDKAQTWMESQLSAVKSGLVGVYAANDGTAGGAIAALKGGG
                     VDPLPPVTGQDAELAAIQRVISGDQALTIYKAIKPEAEDAAKNAVALAGGGAVTSSTD
                     KDGVPSTLLEPVVVTKDNIMDTIVADGFYTVTDICTGEFAAACTAAGIK"
     gene            complement(17912..19099)
                     /locus_tag="PKU75_03670"
     CDS             complement(17912..19099)
                     /locus_tag="PKU75_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPF80005.1"
                     /translation="MNSASPAPGSQSSLRERNLLRVLDTVTSLGSLTQVEIAEQTGLS
                     AATVSNMVRDLHAAGVVSLTPGTRNGRRAVHVALATPDGVIGAMVFGDRDVRVAVAAS
                     PERILSSTRMPLPAEHAADEGLNRAAILIADLLEQLGHEPSELLAVGVGIPAPIDSVS
                     GQVGSSGILPGWRGVDIEVAAGDALRTPTQVDNTANLAAIGELNAGALQGVQDAIHVK
                     MSYGVGAGLIIDGSLYRGSAGTAGEIGHVTIDDRGAVCRCGNRGCLDTVVGSAAILDA
                     LRPTHGGVTLKDVISRALAGDTACQRVLGDAGDALGSALSAVVNLLNPQMISVGGQMA
                     RVGELLLGPARRTIERCALPSAGQSVTIVPGVLGDEADVIGALHLAHEMRETAQRAGL
                     ALA"
     gene            19266..20609
                     /locus_tag="PKU75_03675"
     CDS             19266..20609
                     /locus_tag="PKU75_03675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPF80006.1"
                     /translation="MAQFVVMPKAGNSVESCILLSWQVAVGDAVEEASILCEAETDKS
                     TIEVPAGVSGTVLALIADPGDEIPVMDVIAVVGEPGESFELPESAGPPVPEQAAPPTA
                     SAPAAASAASDAPAPALEAAAGESSVAASPYARSLAQDRGVDWRLVPGSGPHGRVIAR
                     DVETAPATTRGARGLTGAAAGMPEGTGLGGRLTRADLAASPPAAAGLAAADGEFPGAV
                     TESRLTGVRKIVAERMMHSVSSTATVTYTSSAPASALLALRARFKASDPAMGVDAVTL
                     GDLVNFAAVRAATRHPVFSAHLEDGILRTFEDVHLGIAVDTPRGLLVPTVRFASRMGL
                     RELAAHTKSLIGEAQSGGINPDLLSGATFTVSNLGAFGIESFSPIINAPQTAILGVDA
                     ISTRAAIGAAGELVAEQRIGFSLTADHRVIDGADAARFLRDLTTVIAEIDLAIVG"
     gene            20621..21994
                     /gene="lpdA"
                     /locus_tag="PKU75_03680"
     CDS             20621..21994
                     /gene="lpdA"
                     /locus_tag="PKU75_03680"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPF80007.1"
                     /translation="MSDYDVIIIGGGPGGYLAAERLGHAGMRVLLAEADTVGGTCLNV
                     GCIPTKSLLHSAKTYRHALDGAMFGVNVTGVQADWSAMQAWKAKTVGTLVGGVQANLR
                     RLGVTIVHEHARVVGAGRVEVAGSVHKADHVIVATGSEPVMPPIPGADGRVVDSTGLL
                     AVEVIPERLVVIGGGVIGVEFASLFAALGSQVDVVELLPEILPFMDTDIASRVRAGIE
                     GVTFHLGCRVESLDGEPGSTTVRFTDADGSEQALTADTVLMAVGRRPRIADSGAAEAG
                     LDLVRGAIRVDDTMRTNLPGVWAVGDVTGRSLLAHAAYRMAEIATANILSLGRVPPGE
                     RLREDTIPWAVYAIPESAGVGLSESQAEARGLDVTSAVVPLYLSGRFVAENGVGAPGA
                     VKLVAETGSRVVRGIHMLGTYAPEMIWGAVSVLENELTIDDLRQIVFPHPTVSEGIRE
                     ASWAIFS"
     gene            22029..>24015
                     /locus_tag="PKU75_03685"
     CDS             22029..>24015
                     /locus_tag="PKU75_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPF80008.1"
                     /translation="MTKSLIVDPSVVRRHDVVALRELPVNAYEMNWAAEVERLGKDGM
                     RDVLRDMVTIREFESMLDSLKKTGSWLGLEYNHRGPAHLSMGQEAAAVGQAMALEPVD
                     FVFGSHRSHGEILAKCLSAIRKTDADQLAATMEGFLDGDTLRYAEKLPSQGLEEVALN
                     FVLFGTLAEIFARQSGFNRGMGGSMHAFFTPFGSMPNNAIVGGSAPIALGSALFKRIN
                     RQPGIVIANIGDASMGCGPVWEAMTFAAMDQFRNLWREEDGGNPPIIFNFFNNFYGMG
                     GQTSGETMGFGTLARVGAGVNPEQMHAERVDGYNPLAVADAIRRKKEILLEGRGPVLL
                     DTITYRFAGHSPSDASSYRTRDEVELWEHADPIHAFGELLVSKKVMTQGAIDTMREEV
                     RDRLLEVLRIATDEDLCPRVTDRFIESVMLSNGNEPALAEGEPELSEPVSENARTKSL
                     ATKVRAAHDSDGKAVSKIKMFTFRDALFEAMAHGFATDPTMAAWGEENRDWGGAFAVY
                     RGLTEMLPYRRIFNSPISEAAIVGAGVGYALSGGRAVVELMYCDFLGRAGDEIFNQAA
                     KWQSMSAGLLKLPLVIRVSVGSKYGAQHSQDWSALVAHMPGLKVYFPATPTDAKGMLN
                     LALRGTDPVVFLESQLLYDKGEEFEACGVPEGYYETPE"
BASE COUNT         3777 a         8066 c         8310 g         3862 t
ORIGIN      
        1 tagagcttct tggtgggcag gtcgcgccaa ccgtggagcg gcacgggaat gccccagtcg
       61 atgtcgcggg tcatggcgcg cggcttgatg tcgtcgagga tgttgaggct gaacttgatg
      121 acattggggc gccaggtgcc ggaggcctcg cgctcctcga gataggtgcg cagggcgccg
      181 gcgagcgcgg gcaggtcgag gaagaagtgc tgggtctcga cgaaatccgg tgtctcgccg
      241 ttgatcttcg acttcgggtc gatcaggtcc tcgggttcga gctggttgcc gcagttgtcg
      301 cactggtcgc cgcgggcctc tgtgtagcca cagatcgggc aggtgccctc gatatagcgg
      361 tcgggcaggg tgcgcccggt cgaggggctg atggcgccct tctgggtgcg ctcgatcatg
      421 tagccgttct tccacacctg cgaaaagagt tcttgcgcaa cggcatagtg gttggccgtg
      481 gtcgtgcggg tgtagagatc gtaggagcag ccgaggtcgg ccagctcgtg ggcgatgatc
      541 gcgtggttga cgtccacgaa ttcccgtgcg gtgacgccct gcttgtcggc gagcaccagg
      601 atcggggtgc cgtgctcgtc ggagccgctg accatcagga cctcgttgcc gctcatccgc
      661 atgaagcggg cgaagacatc ggagggcacg ccgaagccgg cgacatgccc caggtgacgc
      721 gggccgttgg catacggcca cgcgacggcg gcgagaacct tggtggtcat ggaaccaaat
      781 tttaggttcc ccggccgacg gttctgaccg gttttcgccg gtgctctcga actctgctgg
      841 gatccctgtc gaatccgttc tctcccaacg gttttagggt atgccgtggc cccccgccct
      901 ggtcccgcag ggtgcctctt cgccctggcc ccacgcgccg ggtagttgaa gaattgacga
      961 cgccgcggat ttgccccgcc cccgaaaagt ggtgttgggt cgttactcgg cagggcacgt
     1021 aaccatccgt gaaaccaggg cccggaccgg ccaacctgcc acggatccgg ggctaccaga
     1081 ggggatcaac cagcatgaag cgcatgacca gcatcgtcgg cggactcgcc cttgcgggga
     1141 gcctgggggt cggcaccgcc acctcggcga acgccagcac cgactccatc cgcgccgccg
     1201 gcgtcagcgt ctcggcgggc gacgccaaga ccgccgtcag ctgcccggtt cgctacatca
     1261 cccgcggcat ccgcgtgacc tgtccggcct ccattccggg ctacgccttc cgggccgtcg
     1321 tgaagtgcaa gcgcgccaat ggcaccacct tcctgcgcaa ggacgcctgg cgcgtgcagg
     1381 gcaccggcta cagccaggcc cgctgcccgc tgggcaccaa gcgggtctac ctgccgtacg
     1441 ccttcgagta caagtaagcc gagtattcgc gtacaagtaa gccgagtaga tgaaagggcc
     1501 ggcaactctc gcgagttgcc ggccctttcg tattgccccc ttacggggca ctcaggcgta
     1561 ggacacgccc gcgtcgtcca agtcctgacg ccgtcgttgt cgccacttgc gcaccttgcg
     1621 ccagacccag aacatcatcc cgaagagcaa cagcagcgcc aagatcacca ggatcgggat
     1681 gaagatcgcg atgagcgaca gcccgatcga ggcgccgtcc tccatggtgg agacgaccgg
     1741 agccccggca ccgagggtgc cggcgttgat ggccggccgg gcgaccatct tgcccgagtg
     1801 caccagcccc gagacgatga tcccgaggac gatgccgacc cactggttgt cctgcatcca
     1861 ctgggatttg tcgatcgtct ccgcggcgga ggtggcagca aagacgagcc cgcccatgct
     1921 gggccggata aagctctgca ccgcgtcatt gatggtgtcg acggcgggaa tcttgtcgac
     1981 ggccacctcg gtgagcagca gcaggctgcc gatgccgatc gcccaccagc tctccatcca
     2041 gctgaacgag ctgggcagcg tgagcacgtc ggtgaagcgc gcgatcagcg cgacgatgag
     2101 aaaaggtacg taggcgttga ggccggctgc ggccgagagc ccgatgcccg tcaaggcggc
     2161 gatcatgacc cgagagtagc tttctgggag cgggaaagac gtcgcgacgc ccaacgccac
     2221 accctgcggg tcctattccg ccatcccggc gagcgcccgc tcgtggcgcc tcggatcacg
     2281 ccgacagcag ggcgatgagc tggcgcgtgg cgccttcgac gtaggccgcg cgctcgccct
     2341 cgggccgcag cagcgacgac atgagcaccc cttggaaagc ggcgacggtg atctcggccc
     2401 gctcgttcgc gtggtccggc gccacctcgc aggcgatccc catgaccagc cccaccagct
     2461 tggtccgcca ggagtcgaag aactccccca gctgcggcga gcgcgaggac tcgacgccga
     2521 gttcgacctg cacgcgcacc aggtcggccc gcgcggcctg ccccaccacg agcgcgacga
     2581 tctcgtcctc tatgcgctcg tcctgccccg gatccgccag gtccagggcc gcgatccgca
     2641 gccgggcggc gtcgaccgcg gcggccagct ggcccgagac gtgcgcggtc agcgccgaca
     2701 gcagggccag ccgggtgcgg tagtagccgc tggtcgagcc ctcgggcacg cccgcctccg
     2761 cgtcgacggc ccggtgcgtc agcccgcgta ggccgctgcg cgcgacgacc gccgcggccg
     2821 cgtcgagcag atccttgcgc cgcccggtga cgggggcggg cagaccggct ggggcgacgt
     2881 cgcgaggcac tcggccattg tgccctcgtc cagcggttgc ggaatattca ctacatctgt
     2941 agtgttgtca tcagtaccac tacaaatgta gaagaacagg agtgagcatg atcctcagcc
     3001 acgcagccgc cgtcgctact ttcctcaccg tcgtcctcgc gatcgccggc gccctcgtcg
     3061 cgctcgccct cctcgcggcc gccggcgact tcctcgcccg caaccacacc atgcgggtgc
     3121 gccgccacca gagcgtcccg gtctactacc gccacctcgt caccggccac tgatccggcc
     3181 actggtcgac gagccggccg cccagcgcgg catacagctc gcatccagct tcggcgaggc
     3241 acacttgccc gtatgccgtc gctcaccgcc gccgagcccg gtccactgcc gtggaccggg
     3301 ctttcgcgtc gccacctggt cctcgagacc tggctggtgc tcggggtctc gctcggtgcg
     3361 tcggcgatct ggtcgctgct gcggatcatc gaccggctga ccgtgcacgt cgcgctgaac
     3421 cagcagacga cgagcatgaa caactcggtc acgcccgacc gcccctggct cgacctggcc
     3481 taccagctga ccggcgtcat cctcggggtg gtgcccgccc tcctcgccct gcacctgctg
     3541 cgccgcgacg acgccggcgc ctggcgcgcg atcggctggg accggcaagc gccgggccgc
     3601 gacctgctgg ccggtgtcgg gctgaccgca ctgatcggca tacccggctt gggtctgtat
     3661 gccgcggccc gggccctgaa cctcaacacc acgatcgcgg cggcgaacct caccgaccac
     3721 tggtggacga tccccgtgct cgtcctggcc gccgcccaga atgccgtcct cgaagaggtc
     3781 gtcatggtcg gctatctgct gacgcgctgg cggcaggccg gctggccggc cccgctcgcg
     3841 atcgccatct cggcgctggt gcgcggcagc tatcacctct atcagggctt cggcgggttc
     3901 gccggcaatc tcgtcatggg cctggtcttc ggctacctct acacccgcac ccgcagagtg
     3961 ttgcccctcg tcatcgcgca caccctgctc gacgtcttcg ccttcgtcgg ctacacgctg
     4021 ctgaaggatc acttgtcctg gctgtaaatc ccccgccctt gtcggcgccg actggcagac
     4081 ttcccgcgtg ctgcggcgac tgggcttccc cgacttctcc caccacaagg cgctggtgac
     4141 cggagtcggc atcgacgcgc tcggcagcgg cgtgttcatg ccggtctcca tcctctactt
     4201 cctgatgacc accgaggtcg gcaaggccca ggtcggcctg gcgatgagca tcgctgcggc
     4261 gctggccgtc cccttcgtcc tcgcggtggg cgggctcgtc gaccggcacg gtgccaagac
     4321 gatcatcctg gcgggcaacg tgctgcaggc ggtggccttt gtcggctacc tgtgggcgca
     4381 ggacttttgg accatcgtcg ccgtccaggc gttggcgagc ctggggcaga gcgccttctg
     4441 gggcgcctac tccccgctcg tggccgcctc gactccaccg gatcagcggg aagtgtggtt
     4501 cggcttcctc ggggcgctgc gcaatgtcgg gttcgccatc ggcggcctgg cgtcgggcat
     4561 cgtcgtcacg atcggcacga gcgcggccta tcacgccgtc gtgctcgtca acgccttcag
     4621 ctatatcctc gccttcgccg cgctcgcgaa ggtcccgggc gggcgggcgg cgagcgacgg
     4681 cccccaggac gccgatgcgc cggcgcgcgg cggctggggc gtcctggccc gcgacggacg
     4741 ctatctcggg ctggtcggcg ccaacgcggc ctacgcgctg tgcacggtct cgctcaatat
     4801 cgccatcccg gtctacgcca tcgacgtgct caagctgccc ggctgggtca gcggggcgct
     4861 gttcgtcgtc aacacgctca tggtcggctt cggccagggc ctgatcgtgc gccggatgga
     4921 cgggcatcgc cgtcaccaga tcgtcatcct cggttttgtc ctgtatgccg cgggcttcct
     4981 cgccctcgcc gcgagcggca ccctgccggt cgcgctggcc accgccgcga tcccggtctc
     5041 ggtcgccatc tacaccctcg gtgaactgct cggcgggccg ccgctgtccg ctgcggcggt
     5101 cgacggcgct cccgagcggc tacgcggacg ctacctgtcg gcataccagc tgtcctgggt
     5161 cgcctccggc atcatcgcgc cggccctcta tctcgccctg ctcgcccacg accgtttcag
     5221 cgtgtgggcc accctgatcg gcatcgccgc gctcggctgc gtgggtctgc gagcgcttgc
     5281 tcctcggatg ccggccgtga gcgccgtcgt caccagtgct caggtgaccc acgcctgaga
     5341 accgaagcga cactgatcat cgggacgccg catagatgcg gcgcacgacg tcctcgatgt
     5401 cgggctcttc gatggccagg tcgagcacgt cggcgcgggc cgagacggcg gcgagcacgg
     5461 ccgcggccga ggtcgcctgc gggtcgaacg cgaggcgctg ccgtttgccc tcgccctcga
     5521 cccccaccag ggccgcaccg gggatgtcgc gcaactccgc gaccccctcg tgccccgcct
     5581 gcggcgcgag gtcgacgacc atgacgcgtt cggccccgac ggttttcgcg agtccggaga
     5641 gggtgccgtc ataggccagg cgcccgtggt cgacgacgag cacccggtcg cagaggcgtt
     5701 cgatgtcgcc catgtcgtgg gtcgtcagga gcagggtcgt gccgtgttcg cgccgctcgg
     5761 caccgaggaa gtcgcgcagc cgctgcttgc tgagcacgtc cagtccgatg gtgggttcgt
     5821 cgaggatgag caggcgcggc cggtggatca acgaggccgc gatctcggcg cgcatccgct
     5881 gcccgaggga gagctgccgc accggggtgg acaggaagcc gcccatctcc agcaggtcca
     5941 ccagttcacc ggagcgcacg cgcgcgactg ctggatccag gcggtgaatg gcgccgagga
     6001 tcgtgaacga ctcctggaga ggcaggtccc accacagctg gctgcgctgg ccgaagacga
     6061 ccccgatctc gcgggccagg gcgatccgct cacgcagggg gtcgcgcccg caggtgcgca
     6121 ccagtcccga cgtgggccgc aggatgccgg tgagcatctt gatcgtggtc gacttgccgg
     6181 caccattggc cccgatatag ccgacggcct cgcccggttc gacggtgaag ctgatgtgat
     6241 cgacggctcg cagcttcttg tactcccgca cgaggtcccg ggtctcgatg atctgcgtgg
     6301 ccatcagcca cctcctcctt ggtaatgccg tgtgccgaat cgccagacga gccccgccag
     6361 cgcacagctg gccagggctg ccgccggcga ggcccacgcc agccagcgcg gcacgaagcc
     6421 gtcgccgggc aggtcgagca ggacgatcgt cggcaggtag gcgacgaagg ccgtggggat
     6481 gacgaaggtc cagaagatcc gcaccggatc ggggaagatc ggctgcggct gctgcgccga
     6541 ataggacccg ccataggtga agccgttggt gaactccggc ccgttgatga ggaagaactg
     6601 cgcgcccccg gccaccgtga acagggcgga gaagatcact gtcccgctga cgatcgacag
     6661 cacgaggagg gcccccttgg cgggtgacca ggcgacctgc gccatggcca gaccgacgac
     6721 gagcagcacg agagcgaccg ccagccggcc gatgcgtcgc aaggagatat cgctggtcat
     6781 gaactgcgcc agcaccggca acggccgcac gtagtaggcg tccacctgtc cggcccggat
     6841 cacctggggc agggtgtcga ggtgaccgac gagcatgtcg gcgagggaga aacagaggtt
     6901 ggacagggcg aagacgacga tcgcctgccc gtaggtcaac ccgccgaaga ccggcacatt
     6961 gtgaaagacc ccccacacct ccgcgaactc ggtcacgccg acgaggagcg tgccgacgat
     7021 atcggtgacg aaagacgccc ggtactgccg ctgggcccgc atgcgggccc cgagtatgac
     7081 gcggtagggc cgcaaccgtt caccgacgga gagcgtgggc cggccggccg gttcagccac
     7141 cctgcacctc cacccggcgc cggccgcggg cgagcaccca ttgcccgacg accgtgagga
     7201 cgagcaccca gaagagttgg gagacaacgg cgctcaccgc gtccgcgccg gcgatgcgcc
     7261 cgctgaggat gtcgagggga tgctggagca tcgacgggaa cggggtgcac gacgcgacgg
     7321 cctgcaccca cccggggaac cagggcaacg ggagatagag gccgcacagc accccgccca
     7381 ccaccatcca cgccagccgc aacccgcgga tctcggtgaa ccacaaggca ctgaggttga
     7441 gggagaaggc accgaggaag gcgacgacaa cggcgagaag gatcgacagc aaccccagcg
     7501 cagccgacgg catacccggc ggccacgcga ggtcgaacag gagggcgccg atcgccaacg
     7561 tcgggatcgc tcgggccaca aggccgacgc cggcgcgccc gacgtcgccg gccaggtaga
     7621 ccagcatcgg gttgaccggc cggaggaagt cgacggcgat gtcgccgctg cgcacgcggt
     7681 cggcgacgag cgagaagccc cacaggttga tcgtgccgag cagcgcctga ccccaccaga
     7741 cgaacgcggc ggcggtccgc gcgtcatacc ccccgacggc cgcaccggcc gacgcgacgg
     7801 tggccagggc gatggccgcc cggatgatcc cgaagacgct gttcgtcgag atgcccgcca
     7861 gcagcgccag ccggtatgtc gtctgctggc ggaaccccga ccgcaccagc agcgcggtgg
     7921 tgcgcaggac gcgcgtcatc gcggggaggg gaggccaggc acgatccccg agcttatggg
     7981 atcggtcagg cgttgcgcgc ggtcgggtag aggacccgga agcgctggca gaccaccggc
     8041 atgtcgctgg tgaagcccgc gctgctgtat gccgtgaaga agcgctcgtg gatctcgcgc
     8101 cacgattcca gggtcaggtc gccctcaccc tcggacctgg cgtgctcggc gtcgacctga
     8161 tcgaagggca cgatcgacac tgcggtggtc tcgatgacgg cacgcggatg tgccctgccg
     8221 tcgaggatga tcgccaggtc gccgggccgc tccggttcgt cgccgtcggc ctcgtagtcc
     8281 gcgagggcgc ccgcggtgcc ggtcttggtc ccggccagca cgagcgcgag caggtcgtcg
     8341 gcgtgctccg gggtcgcccc gaaggcccac gcgggcggga tgagcgtgct cgcggcagtt
     8401 tcgccggtgt atgtcgccac cgggttcagt cgcagtttga ccttggcgat ctcccagaac
     8461 tgggccagct cgtcagggtc gacgatcggg ccctcgctcg cgggaggctg ttcagctgtc
     8521 atgggacgtc ggcctccgcg tgggatcggt cgaaaaacgg cgtgaaggtg aggcgtcgca
     8581 gcgacgcagg gggaaccggt tgcgtaccac acactcgcgc acctcgatgt cggccatgac
     8641 cccaccatgc cacacgagcg ggattgagcg cggaagcggc caccgtcacg acgacgtgcg
     8701 ctcccgacgt ctttccgtcg agagcgcacg tcgttacgag tgacgctgga ggtccgggat
     8761 cagcgcttcg tgaatgccca cgccgagacg ttcagcgtgt tctccaggcc ggtgaagcct
     8821 tcggcgggga tgtactcgtc ggtgccgaag tcggtgtccg tgtcatcggc ggtgccgccg
     8881 actccgtcgg ggccgacgcc gaagaggtcc acgaagccgg tgccgccggc atccatcagg
     8941 ttggccgttt cgttggtgtt gttggtgtgg tagttgccga tgagatgccc cacctcgtgg
     9001 gccacgacat tgccgaccgc ctgcgagacg aaggcgaccc ggtcgctcga ctcgttcatg
     9061 taggtgttca gcgagtacgc cggtccactc ggctccgaca tcgcgtcgag cagcaccagc
     9121 gcctggtcgt ccatggcgta gttgcccggg tcgatgtact gggcgatgcc gatcgtgctg
     9181 atgccggact cggcgatggt gccgccgacg atgacccggc tgacgcgggc cttgccgtag
     9241 atgtcggcac tgttctgcga gttgacgatc gtgacctgga cgttggggtt gaggcccttg
     9301 gccctcaggt cccacttgag gttctcgttg acctcggtgg tgatcttgtt gatcaactcg
     9361 cgttcctggg acttgctcag accccagctg gagaggaagg cgttgaacgg cgaaagcgtg
     9421 cggacgcccg agccgcccca gatggccgtg ttgacccgct gtccgttgaa gtccaggaag
     9481 atccgctgga ccggccccgg agcgagttgg cccccgggcc gataggcctc gagggcgacc
     9541 tggtagtcac cttgcgcgcc gtcgacctgg agggcgtacc agccggcctg ctcagcgaca
     9601 taggcgagtg cggtcgtgcc cgcaccagga agcggcgagt tcggcgggta gaggaaggag
     9661 gcgtccatct cctccgcgcc caccatctcg gtgccgtcgg ggcggaagac ggtcaggctg
     9721 tcggcacccg agaggccaga cccgccgagc acgtcaccgg gacgcagcca gacggcgtag
     9781 aagtccttgt cgacagtcgt gcgggtcatc gccagcgggt agtccccctc gcgtccaacg
     9841 ccgttgccgg acccggagtc catggggtcg gcggggaagg agccgccgga cttgtacccg
     9901 accacggccg cgtagtaggt gccactctcg gtcacgtcgt acgtcagcag actcgccagc
     9961 cccgatcccg cgtcgtcgtc caccgcgagg agggtgccgt cggcgctgta gaggccgatg
    10021 agggtgtcca gcgtcccgcc ggaggtgtcg aggatcaact gctccccggc aacggcggtg
    10081 atggagtaga agtcgacgtc acccgtgccg tcgccggcgg agccgtgggg cccgtcgccg
    10141 atggtgcccg tcgtggtggt cttctcgcgg ggtcccaagc cggagtcgcc ggcgagcggg
    10201 atcgacccgt tgtcctcggg aaccggagcc agggtggttt cgctgaggct gtactgctgc
    10261 tggcttccca ggatccgcac cctgttgttc ttgccggctg cggtgccgaa gacgccgatc
    10321 cgctccgctt ccgctctgct gtcgttggac tgcgtcccgt cgatctcctt ctcgtcgtag
    10381 atcgcgggag cccgcaggtt ccctccggac gccgcgcgct tgttctcctc gagccgggac
    10441 gaccttgccc tctcggcggc tcccttgcgg agcaccgact tccaatagga gtagtcgacg
    10501 ctggactggt cgaggaccat gccgagatac gggttggttg ccgcagcact gccgctgcgg
    10561 acgctcgacg atttcggcgc gacctgtact ccggcattca gcgatcgagc ctcgtccgcg
    10621 gtcacgcggc catcaccgct cgaggtgccg gcgatgcccg cggtaggggc gacgaccagc
    10681 gcgaatgcgg cggccgagac gacacatgga atcttcttca caaaccctcc ccagggctgc
    10741 tcggttggcg ccgggccacg gggctgtgac ccggaccacc atcggtctgc cggacgttac
    10801 cgctgaccgg cgggggccgc atccggagaa aacactgcat ccggccctgc ggaccggatg
    10861 cattgcggca gaacgcgatt acggaaggta aattcttcct ataggcgtcc tgacttggcc
    10921 ttccgcatgt cggcgttgag ttgcgagatg acatcgagag ggatcccctt ggggcaggcg
    10981 tggctgcatt cgccgatgtt cgtgcatcca ccgaagccct cggcgtcgtg ggtggcgacc
    11041 atggccgcaa cccgctgggc ccgttccggc tgaccctggg ggagttcgcc gagatgggtg
    11101 accttggcgc ccatgaagag catgccggag gcgttagggc aggcggcgac gcaggcgcca
    11161 cagccgatgc actcggcggc catgaacgct cggtccgcgt tctccttggg caccaggatt
    11221 gcctcggcct tgggggcggc accggtattg gcggagatga agccgccggc ctggatgatc
    11281 cggtcgaagg cggtgcggtc gacgaccagg tcccgcagga ccgggaaggc ggtggcgcgc
    11341 cacggctcga tggtgatcgt ctcgccgtcc ttgaagctgc gcatgtgcag ttggcaggtc
    11401 gtcgtcttct cggggccatg cgcgtcgccg gagatcatca ggccgcacat gccgcaaatg
    11461 ccctcgcggc agtcggagtc gaaggcgacc ggctccttgc cctcggcgtt gaggcgctcg
    11521 ttgagaacat cgagcatctc caggaaggac atgtccgggg agatgtccgg gacctggtag
    11581 gtgacgagtt caccccgtgc ggtggggccg tcctggcgcc agatcttcag tgtcagattc
    11641 acgacggtcc ttctacttgt acgagcgctg cttgagctgg atgaattcgt aggcgaggtc
    11701 ttccttgtgc aggacgggcg cgcccatctc gccctcaccc gagccgccgg catactccca
    11761 ggcggcgacg tagaggaatt cgtcgtcgtg gcgcagggct tcgccgtcct cggtctggga
    11821 ctcggcgcgg aagtggccac cgcacgactc gcgccggtgc agcgcgtcga tgcacatcaa
    11881 ctcgccgagc tcgaggaagt cggcgacccg gccggccttc tccaggctct ggttcagcgt
    11941 gtcggcggcg ccgagcacac gcacattgcg ccagaactcc gcccgcaggg agcggatcag
    12001 atcgatcgct ttcatcaggc cggtctcggt gcgctccatc ccgcagtact cccacatgat
    12061 gtggccgagt tccttgtgga aggagtcgac ggaccgttcg ccgttgatcg ccagcaggcg
    12121 ccgcgtgcgg tcctcgaccg acgtgcgcgc ctcggccacc gccgggtcgt tctcgtcgag
    12181 cgtcggatag ggccctttgg cgaggtagtc gcggatggtg ttgggcagca cgaaatagcc
    12241 gtccgcgagg ccctgcatca gggccgaggc gccgagacgg ttggcgccgt ggtcggagaa
    12301 gttcgcctca ccggtgacga acaggcccgg gatcgaggac tggaggtcgt agtcgaccca
    12361 caggccgccc atggtgtagt gcacggcggg gtagatccgc atcggcgtct cgtacgggtt
    12421 ctcgcccgtg atccgggcgt acatgtcgaa gaggttgccg tatttcgcct cgacggccgg
    12481 cttgcccagc cgggcgatcg cgtcggcgaa gtcgaggtag acaccccgcc ggaagtcgcc
    12541 gaccatcggg ccgacgccgc ggccctcgtc gcacatgttc ttcgcctggc gggaggcgat
    12601 gtcccgcggg acgaggttgc cgaaggccgg gtagatccgc tcgaggtagt agtcgcggtc
    12661 ctcttcgggg atctggcgcg gatccttctc gcagtcctcc ttgcgcttgg gcacccagat
    12721 ccgcccgtcg ttgcggaggg attcggacat cagcgtcagc ttggactggt ggtcgccgga
    12781 gacgggtatg caggtcgggt ggatctgcgt gtagcacggg ttgccgaagt aggcaccctt
    12841 gcggtgggca cgccagttcg ccgtgacatt ggagcccatg gcgttggtcg agaggaagaa
    12901 gacgttgccg tagccaccgg acgcgagcac gaccgcgtcg gcgaggtggg tctcgatctc
    12961 accggtgacg aggttgcggg cgatgatccc gcgggcctgg ccgtccgcca cgatcagttc
    13021 gagcatctcg tgccgggcgt acatctcgac ggtgccggcg gcgatctgcc gctccagcgc
    13081 ctggtaggcg ccgatgagca gctgctggcc ggtctggccg cgggcgtaga aggtccggga
    13141 tacctgcacg ccaccgaacg agcggttgtc gagcaggccg ccgtactcgc gggcgaaggg
    13201 cacgccctgc gcgacgcact ggtcgatgat gttggcgctg acctcggcga gccggtagac
    13261 gttggtctcg cgggcgcggt agtcgccgcc cttgaccgtg tcgtagaaga ggcggtagat
    13321 cgagtcgccg tcctccttgt agttcttcgc ggcgttgatg ccgccctggg cggcgatcga
    13381 gtgcgcccgg cgggggctgt cctggaagca gaaggacttc acgtggtagc cggcctcgcc
    13441 gagcgtggca ccggcggccg cgccggccag gccggtgccg acgatgatca ccgacagctt
    13501 gcgccggttg gccgggttga ccagggaagc gtcgaacttg cgcgaggtcc acttgttctc
    13561 gatcggaccg gcgggggcct tggtgtccgc gatcggggcg ccgacagagt agtagtcggc
    13621 gtcgacgcct tcgggcttgt tcagggtctc agtcatgtga tcagtccacg attccgaaga
    13681 ggatgcacag cggcgggatg aggaaggcgg tggcaacgag ggacccgatg aagtgcgcga
    13741 ggaacttcgc gttgcggcgc gccgcggtcg tcgaggtcca cccgagggtc tgggcggcac
    13801 tccagatgcc gtggtggaga tggaatgcga gcgcgatcac ggccaccgcg tagatcagga
    13861 cgagccacca cttgtcgaag ctgttgatga cattggcgta cggcgtcggc tgctcgccct
    13921 gcgggcggat ggtgtgcgtg gtcaggtgca ggaggtggaa gatcacgaag agcagcaacg
    13981 cgatgccgcc gaagcgcatc gtgcgcgagg cgagcgagga cgccgcggcc ttcttcaccg
    14041 catacttcgt gctccgcgcg ccgttggccc ggccccacag agtcagcgcc gagtagacgt
    14101 gcagggcgag cgcggcgatc aacaccagcc ggaagaccca caggaagccc gagtagggca
    14161 gcatcggctc gccgagcgtg cgcaggtgat gggcgtagtt gttgaaggcg tcgggaccgc
    14221 cgagcaccat caagttgccg taggcatgcg caagcacgta gaagacgaag agcaggccgc
    14281 ttccggccat caggagtttg agcccgatgg aggaccgcac ggccggcgtg gacgcaggga
    14341 gggtcgatgt tgccacgggg gaaacctatc gccagagggg ctggtgcgtc tgctcaggtg
    14401 gcccttcgcg agtgtgaaat tgtccctact tttgggtatt tcgcgcccca gctcctcgcg
    14461 acggtcaccg gcgcaggggg ctaggcccga atccgggccg gccggctagg cgcggccgtg
    14521 ggcggcgcgg ctgcggcggg agaggctgtc gatgataacg gcgagcagga ggacgccccc
    14581 ggtgaccatg aacttgtagc tcgtgtccag actgagcagt ccgaggccgt tggagatgga
    14641 ctggatcacc aagatgccca gcagggcgga atatgccgat ccgcgcccgc cgaagaggct
    14701 ggtgccgccg atcacggctg cggcgatggc gttgaggttg gtgtcggcgc cgccgctgct
    14761 ctgactgacc gaggccagtc gggcggcggc gaagacgcca ccgagagcag cgaagacgga
    14821 gcagagggcg aagaccgtca gatagatgcg cttgacgttg atgcccgcgc ggcgggcggc
    14881 ttcgacattg ccgccgacgg ccatgaccga gcggccccac cgggtgcggc ggatgaccag
    14941 gtccatgacg acgaccaggg cgatgaagac gagcaccatg taggagacgc cgcggtcgcg
    15001 gccgaggatg tagacggccg ccagcaggat caccgcgaga atcccggccc gggccagcac
    15061 gacgctggtc ggcgtggccg agagcccggc cgcaacacgc ttgcggccct gagccatctt
    15121 cgcggcgagc gaagcaccga cggccagcaa cacgagcaga taggcgacga catcggggac
    15181 gaacttctgc gaggcgaact gcacgatgtc ggagttgaag gagagattga tcgtgccgtc
    15241 cttgccgagc accttgatct gcaatccgag gaaggccagg agcccggcaa gggtgatgac
    15301 gaaactcggc accccgtagc ggttgaacag caggccatac agcacaccga tgacgacacc
    15361 gacgagcagt gcgactccta tggcgagtcc tgaggtccag ccatggtgga cgaatccgac
    15421 ggcgagcacc gcgccggcca gaccactgac cgacccgacg gacagatcga tctcgccgag
    15481 cagcaggacg agcacgatcc caatggcgat agtgccggtc ggaaccatct gcagggtcag
    15541 gttgacgaga ttgcggctgg agaggaaggc agggttgagg gcatagaaga ccccccagat
    15601 gatgatgagt ccgacgacca cgggcatgga gccgagatca ccggatcgca gtcgcgcaag
    15661 tgagcccttg agccaccccg agaggccttc gtcggcgatg aagcgttcgt cctgccggtc
    15721 agcgggcagc gcgagggtgg ggtcgttggc cggggcgctc acagctgctc ctcgatagtg
    15781 tcctgggtgg gggtcgcgtc ggggtcgcgg cgggccgccc gctccgagac ggcgttgttg
    15841 gtcgccccgg tgatggcggc gatgatctct tggctcgtcg tgtcggccac ccggaactcg
    15901 ccgttgttgc gaccgagccg cagcacgacg acgcggtcgg cgaccgccat cacgtcagac
    15961 atattgtgcg agatcaagat gaccccaagt ccgttctccc gcaggcgttc cacgagattg
    16021 agcacctcgg ctgtctgggc aacaccgagc gcggccgtcg gctcgtcgag catgacgact
    16081 ttgggtttgc cgagcaatga ccgcgcgatc gcgacggtct gccgctggcc accggacagc
    16141 gaggccacgg ggatccgcac gctggggatc ttggcggaca gctctcgcag aagccgccag
    16201 gactccttct ccatggcgac ctcgtcgagc atcccggccc cggtcacctc gctgccgagg
    16261 aagaggttgg cgacgacgtc gaggttgtcg cacagggcga gatcctggaa cacggtggcg
    16321 atgccgagac gctgggcctc ggccggggag gtgatgtcga ccttggatcc gtcgaacgtc
    16381 acctccccct cgtcgggctg gtagaccccg gcgatggcct tcaccagggt ggacttgccc
    16441 gcaccgttgt cgccgacgag agccaccacc tctccgggac tcacgtcgaa ttcgatgtcc
    16501 ttgagggcct ggacggcgcc gaagcgcttg gagatccccc gcatggacaa gaccggcgcg
    16561 gccggagagg tggtggtcat ccgtgactca cttcgtgttg gtcgaagaac tacttgatgc
    16621 cggcggcggt gcaggcggcg gcaaactcac cggtgcagat gtcggtgacc gtgtagaagc
    16681 cgtcggccac gatcgtgtcc atgatgttgt ccttggtgac gacgaccggc tcgagcaggg
    16741 tcgatgggac accatccttg tcggtcgagg acgtcacggc cccgcctccg gcaagggcca
    16801 cggcgttctt cgccgcgtcc tcggcctcgg gcttgatcgc cttgtagatc gtcagcgcct
    16861 ggtcgccgct gatgacccgc tggatcgcgg ccagttcggc gtcctggccc gtgaccggtg
    16921 gcagcgggtc gaccccgccg cccttgaggg cggcgatggc gccgccggcg gtgccgtcgt
    16981 tggcggcata cacaccgacg aggccggact tgaccgcgga cagctgggac tccatccacg
    17041 tctgtgcctt gtcggggctc cagtccgggg tgtcgtattc ggccttgatc gtgaacccgc
    17101 tcttgtcgag aacgctgtgt gcgccggcct tgaagtcggc cgcattggcg tcggtgggcg
    17161 agccgttgat catgacgatg tcgccgctgg tcttgccggc cgccttgagg gcatcgacca
    17221 aaccggtgcc ctgcaactcg ccgaccttct tgttgtcgaa ggagacgtag tagtcgctgc
    17281 ccgcgacgaa gcggtcatag gcgatgactt tgacgccctg tcccttggcg ttggcgacga
    17341 tggtctgggc ggacttgccg tcgacggcgt cgaggacgag caccttggcg ccctgggtca
    17401 gcgcggcctc ggcctgttgc tgctgtttgg cggcgtcctg gtcggcattg gagtagagga
    17461 tctcgcagtc ggcgcaggcg gccttgaccg ccgcctcgaa cagcgggcgg tcgaaggtct
    17521 catagcgggt ggtcttcgac tccggcagca gcagggcgat cttgcccccg ccggcgccgg
    17581 cgctggatgc cgattcggag gccgatgcgg acgccgaccc cgaaccagag gtggccgccg
    17641 ccgaccccga actggtcgag gaggtggtgg aggaattcgt gccgcaggcg gcgatggccg
    17701 tcacggacat cgccgcgacg atggctaggg atgcggcgcg cggggcgcgg agagtgtgca
    17761 tcgggtctcc tgatcggtgt cggttgcgac ggctgtgtca caactcagcg gaattcaact
    17821 ccggacactc tttgtcgtca agaagtgaag acaaagctgc ccgaactgtg accgacggat
    17881 cactcggcgg cacgatcacc gcgggacccg ttcaggccag ggcgagccct gcgcgctgcg
    17941 ccgtttcccg catctcgtgg gccagatgga gtgcgccgat gacatccgcc tcgtcgccga
    18001 ggactccggg gacgatcgtc accgattgcc cggcactcgg caaggcgcag cgttcgatcg
    18061 tgcgccgtgc cggcccgaga agcaactcgc cgacgcgcgc catctggcca ccgaccgaga
    18121 tcatctgtgg gttgaggaga ttgaccacgg ccgacagggc agaccccagc gcgtcaccgg
    18181 cgtcaccgag aacccgttga cacgcggtgt cccccgccag ggcccgggag atcacgtcct
    18241 tgagggtgac accgccgtgg gtcggccgca gggcatcgag gattgcggcc gacccgacca
    18301 ccgtgtcgag acagccccgg ttgccacagc ggcatacagc tccgcggtcg tcgatcgtca
    18361 cgtgaccgat ctcgccggct gtgcctgcgc tcccccgata caacgagccg tcgatgatca
    18421 ggccggctcc gacgccatag ctcatcttca cgtgaatggc atcttgaacc ccttgcaggg
    18481 caccggcatt cagttcgccg atggccgcca ggttcgccgt gttgtccacc tgcgtggggg
    18541 ttcgcagcgc atccccggcc gcgacttcga tgtccactcc acgccatccc ggcaggatcc
    18601 ccgagctgcc gacctgaccg gagacggagt cgatcggcgc cgggatcccg acaccgaccg
    18661 ccaacaactc cgagggctcg tgcccgagtt gctcgagcag gtcggcgatg aggatggccg
    18721 cgcggttgag accctcgtcg gcggcgtgct cggcgggcag gggcatccgc gtgctactga
    18781 ggatccgctc cggcgacgcg gccaccgcga cccgcacatc gcgatccccg aagaccatcg
    18841 cgccgatgac cccgtcgggc gtcgccagcg ccacgtgcac cgcgcgccgg ccgttgcggg
    18901 tgccgggcgt gagtgagaca accccggctg catgcagatc gcgcaccata ttggagacgg
    18961 tggccgcgct caggccggtc tgttcggcga tttcgacctg ggtgagcgag cccaagctgg
    19021 tcaccgtgtc gagcaccctc aacaggttgc gttcgcgcag cgaactctgg gagccggggg
    19081 cgggactcgc tgagttcacg tcctaaagct aagcagaagg tcaggtgcct ggggaagggt
    19141 cccccgcggc acatacgatc gaggtatcgc ttccgagtca cggcatccgg cctgacttca
    19201 cgaggttcgg caggcccgca atgggcctcg atgcgtacgt cgagggtcga agagaggacg
    19261 ctttcatggc gcagttcgtg gtcatgccga aggccggcaa ttcggtcgag tcctgcatcc
    19321 tgctgtcctg gcaggttgcc gtgggcgatg ctgtcgagga ggcctcgatc ctgtgcgagg
    19381 ccgagaccga caagtcgacg atcgaggtgc cagccggcgt cagcggcacg gttctcgcgc
    19441 tgatcgccga tccgggcgac gagatcccgg tcatggacgt gatcgcggtc gtcggtgaac
    19501 ccggcgagag cttcgagctg cccgaatcag ccggcccccc tgtgcccgag caggccgctc
    19561 ccccaacggc atccgcaccg gccgccgctt cggccgcctc ggacgcgccg gctcccgcgc
    19621 tggaggcggc ggccggtgag tccagcgtcg ccgcgagccc gtatgcccga tcgctcgcgc
    19681 aggatcgcgg cgtcgactgg cgactcgtcc cgggcagcgg cccgcacggc cgcgtcatcg
    19741 cccgggatgt cgagacggcc ccggccacca cccgcggggc gcgcggtctc accggtgctg
    19801 ccgcgggtat gccggagggc accggtctcg gcgggcgtct cacccgggcg gatctcgccg
    19861 cgtcgccacc ggctgccgcg ggcctcgccg cagccgacgg cgagttcccg ggagcggtca
    19921 ccgagtcccg gctcacggga gtgcgcaaga tcgtcgccga gcggatgatg cactcggtct
    19981 cctcgaccgc gaccgtcacc tatacgtcat cggcaccggc gtcggcgttg ctcgccctgc
    20041 gggcccggtt caaggcgtcc gaccccgcga tgggtgtgga cgcggtgacc ctgggcgacc
    20101 tggtcaactt cgcggccgtt cgcgcggcga ctcggcaccc ggtgttcagc gcacacctcg
    20161 aggacggcat cctgcgcacc ttcgaggacg tgcacctcgg catcgccgtg gacacccctc
    20221 gcgggctgct cgtgccgacg gtccggttcg cgagccgaat ggggctgcgc gagctggcgg
    20281 ctcacaccaa gtcgttgatc ggcgaggccc agtcgggcgg aatcaacccc gacctgttgt
    20341 ccggggcaac cttcaccgtc tccaacctcg gtgccttcgg catcgagagc ttctccccga
    20401 tcatcaacgc gccccagacc gccatcctcg gtgtcgatgc gatctccacc cgtgccgcga
    20461 tcggcgcggc cggcgagctg gtcgccgagc agcggatcgg gttctccctc accgccgatc
    20521 accgagtcat cgacggagcg gacgcggccc gcttcctgcg ggacctcacg acggtgatcg
    20581 ccgagatcga cctcgcgatc gtcgggtgag caggcgagca atgagcgact acgacgtcat
    20641 catcatcggt ggtggccccg gcggctacct ggccgccgag cgtctgggcc atgccgggat
    20701 gcgggtgctg ctggccgagg ccgacaccgt cgggggcacc tgcctcaatg tcggctgcat
    20761 cccgaccaag agcttgctgc actcggccaa gacctatcgc catgccctcg acggcgccat
    20821 gttcggcgtc aatgtcactg gcgtgcaagc cgattggtcg gccatgcagg cctggaaagc
    20881 caagaccgtc gggaccctcg tcggcggtgt gcaggcgaac ctgcgccgcc tcggcgtgac
    20941 gatcgtccat gagcacgccc gggtcgtcgg agccgggcgg gtcgaggtcg caggcagcgt
    21001 ccacaaggcc gaccatgtca tcgtggccac gggatccgag ccggtcatgc cccccatccc
    21061 cggggccgac ggccgcgtgg tcgattcgac cgggttgctc gccgtcgagg tcatccccga
    21121 gcgcctcgtc gtcatcggcg gcggggtcat cggggtcgaa ttcgccagcc tcttcgccgc
    21181 gctcggctca caggtcgacg tcgtcgaact cctccccgag atcctgccgt tcatggacac
    21241 cgacatcgcc tcgcgggtgc gggccggcat cgagggcgtc accttccacc tgggctgccg
    21301 tgtcgaatca cttgacggag agccgggttc gacgacggtg cgcttcacgg acgccgacgg
    21361 gagcgaacag gccctgaccg ccgacacggt gctgatggcc gtcggtcggc gtccccggat
    21421 cgccgactcc ggcgcggccg aggccggcct ggacctggtc cgtggcgcga tccgtgtcga
    21481 cgacacgatg cgcaccaacc tcccgggcgt atgggccgtc ggggatgtga ccgggcgcag
    21541 tctcctcgcg cacgcggcat accggatggc cgagatcgcc acggccaaca tcctcagcct
    21601 cgggcgggtg ccgccgggcg aacggctgcg cgaggacacc atcccgtggg ccgtctacgc
    21661 cattcccgag tccgcgggcg tcggcctgag cgagtcccag gccgaagcca gagggctcga
    21721 cgtcacctcc gctgtcgtgc cgctctatct ctcggggcgc ttcgtcgccg agaacggcgt
    21781 cggggccccg ggcgccgtca agctcgtcgc cgagaccggc tcgcgggtgg tgcgcggaat
    21841 ccacatgctc ggaacctatg cccccgagat gatttggggc gccgtgagcg tgctcgaaaa
    21901 cgaattgacc atcgacgacc tgcgccagat cgtcttccct caccccaccg tctccgaggg
    21961 catccgcgag gcgtcatggg ccatcttcag ctaagccgtc cgcccctgcc accgaaggta
    22021 gatacgaaat gaccaagtcc ctgatcgtcg acccgagcgt cgtacgccgg cacgatgtcg
    22081 tcgcgctgcg cgagctcccc gtcaacgcct atgagatgaa ctgggccgcc gaggtcgagc
    22141 gactcggcaa ggacgggatg cgcgatgtgc tgcgggacat ggtcactatc cgcgagttcg
    22201 agtcgatgct ggattcgctg aagaagaccg gcagctggct cggcctcgaa tacaaccacc
    22261 gcggccccgc ccacctgtcg atgggtcagg aggcggcggc ggtcggccaa gccatggcgc
    22321 tcgaacccgt cgacttcgtc ttcggctccc accgcagcca tggggagatc ctcgccaagt
    22381 gcctgtcggc gatccgcaag accgacgcgg accagctagc cgcgaccatg gagggattcc
    22441 tcgacgggga cacgctgcgg tatgccgaga agctcccgag ccagggcttg gaggaggtag
    22501 ctctcaactt cgtcctcttc ggcacgctcg ccgagatctt cgcccggcag tcgggcttca
    22561 accgcgggat gggcggctcc atgcacgcct tcttcacccc gttcgggtcg atgcccaaca
    22621 acgcgatcgt gggtggctca gcaccgatcg cgctcggctc ggcgctgttc aaacggatca
    22681 accggcaacc ggggatcgtc atcgccaata tcggcgacgc ctccatgggc tgcggccccg
    22741 tctgggaggc gatgaccttc gcggcgatgg accagttccg aaacctgtgg cgcgaggagg
    22801 acggtgggaa cccgccgatc atcttcaact tcttcaacaa cttctacggc atgggcggac
    22861 agacctccgg cgagaccatg ggcttcggca cccttgctcg cgtcggcgcc ggcgtcaacc
    22921 ccgagcagat gcacgccgaa cgcgtcgacg gctacaaccc cctcgccgtg gccgacgcca
    22981 tccgccgcaa gaaggaaatc ctcctcgagg gccgcggacc ggtgctgctc gacaccatca
    23041 cctaccggtt cgccggccat tccccgtccg acgcctccag ctatcgcacc cgcgacgagg
    23101 tcgagctgtg ggagcacgcc gacccgatcc atgccttcgg cgagctgctg gtgagcaaga
    23161 aggtcatgac gcagggcgcc atcgacacga tgcgtgagga ggtgcgcgac cgcttgctcg
    23221 aggtgctgcg catcgccacc gacgaggacc tgtgcccgcg ggtgaccgac cggttcatcg
    23281 agtcggtcat gctctccaat ggcaacgagc ctgccctcgc cgagggcgaa cccgaactct
    23341 ccgagccggt ttcggagaac gcgcgcacca agtcgctggc gaccaaggtc cgcgcggccc
    23401 acgattccga cggcaaggcc gtgtcgaaga tcaagatgtt caccttccgc gacgccctct
    23461 tcgaggcgat ggcgcatggc ttcgcgaccg atccaaccat ggccgcgtgg ggcgaggaga
    23521 accgggactg gggtggcgcc ttcgccgtct atcgcgggct gacggagatg ctgccctatc
    23581 ggcgcatctt caactccccc atcagcgagg ccgccatcgt cggagccggt gtcggctatg
    23641 cgctttccgg cggtcgcgcg gtcgtcgagc tgatgtactg cgacttcctc ggtcgcgccg
    23701 gcgacgagat tttcaaccag gccgccaagt ggcagtcgat gtcggctggg ctgctgaagc
    23761 tgccgctcgt tatccgcgtg tccgtgggta gcaagtacgg cgcccagcac tcgcaggact
    23821 ggtcggcgct ggtggctcac atgccgggtc tcaaggtcta ctttcccgcc accccgaccg
    23881 acgccaaggg catgctcaat ctggcgctgc gcggcaccga ccccgtcgtc ttcctcgaaa
    23941 gccagttgct ctacgacaag ggcgaggagt tcgaggcctg cggggtgccg gagggctact
    24001 acgagacccc cgagg
//
LOCUS       DAOPJG010000021        23987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2231_length_23987_cov_7.542997, whole genome shotgun sequence.
ACCESSION   DAOPJG010000021 DAOPJG010000000
VERSION     DAOPJG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 23987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23987
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2231_length_23987_cov_7.542997"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PKU75_03690"
     CDS             complement(<1..1013)
                     /locus_tag="PKU75_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPF80009.1"
                     /translation="MVPWLLLLAAIVLTVGTALFVATEFSLVALDRPTVQRAVDQGDH
                     EAQTVLTSLRGLSTQLSAAQVGITLTTLVLGFIAEPSIATILRPAIDAVGVPEGALDT
                     VSSAVALLLATLFSMVFGELVPQFLGISAPLRVGKKVAGPTRVFALVAKPLIAVLNGS
                     ANAFLRAVGVEPQEELSAARTPQELASLVGRSVQAGTLDEGTGRLVAASLRFGAQTAE
                     DVMTPRGRAWNIERTASAADVVELARQTGHSRFPVTAGDWDDVDGIVHVKAAISVPRG
                     RRAAVPVSALMTPPVVVPETLRLDPLLDLLRQSGPQFAVVVDEYGGTAGVVTLEDVVE
                     EIVG"
     gene            1125..2111
                     /locus_tag="PKU75_03695"
     CDS             1125..2111
                     /locus_tag="PKU75_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF80010.1"
                     /translation="MSVAATTTGTSIEIDGLSKRFGDFLAVDNLTFRVQPGRVTGFLG
                     PNGAGKTTTLRMLLGLVRPTGGTATIGGRAYQTIPEPIRTVGAALEATNFHPGRTGRD
                     HLRVLAATAGIPDTRVDELLELVGIPAAARKRAGGYSMGMRQRLGLAVALLGDPHVLV
                     LDEPANGLDPEGIRWLRGFMRHLAGQGKTILVSSHMLQEVEQTVDDVVIISNGRLIRS
                     GTMAELHGPATSLVRTSDPGALAGALRVADVTSHTADDGGLIVETDDLRLIGDVALRA
                     GLPLWQLQPQVSDLEDMFFALTEGQNRNLGATDAAAPHEMDAIAGRDGGNEK"
     gene            2108..2950
                     /locus_tag="PKU75_03700"
     CDS             2108..2950
                     /locus_tag="PKU75_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF80011.1"
                     /translation="MIGAIRSEIRKVFTTRLWWGMGLGMAVLAALISMGFAALLGNAA
                     MTGGDNNGQGNPFANMTVGTAQLIYNAGIIQQMTILFPLALGVLLITSEYRHKTISAT
                     FLSTPNRWVVLLSKMVAVAVTGALYAVVHAIASVAGGASIIKFVKNQPTMLGETEVWN
                     SLGIGLIAFAIWMLFGFGVGMLIRNQIAAVLLAVGATFVVQIALNIIFSVKEWYSAMK
                     WIPGNLTANMVITSDPTAGQTVDPATQSQYFAHWWQAGLVLAAYAAVLAVVGAWLTTR
                     RDVA"
     gene            3118..6996
                     /locus_tag="PKU75_03705"
     CDS             3118..6996
                     /locus_tag="PKU75_03705"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HPF80012.1"
                     /translation="MADPTPANPMEAFGPNEWLVDELYESYLKDRNSVDRAWWDFFST
                     YKLEHPHGAGGNGTQPAAPAARTSTAPQAPKSAPPAPTTNPAATENPQSTTAETGPTR
                     PGASGPGSAAQAPVPADAPAPKPEMRAPERPTPTGPNATPRDAAKPAAAKATEEPTVS
                     PIRGASARVVTNMEASLEIPTATSVRSVPAKLLMDNRIVINNHLRRTRGGKVSFTHII
                     GYALVKALAQMPEMNNSFTEENGKPALSVPAHVNLGIAIDLAKPDGTRQLLVPSIKAA
                     ETMDFGRFWSSYEDIIKKARGGKLVVEDFMGTTISLTNPGTIGTVLSVPRLMKGQGAI
                     IGVGALEYPAEWQGASTTVLHRHGVSKILTLTSTYDHRIIQGAQSGDLLRIIHNLLLG
                     QDGFYDEIFESVRIPYEPVRWVQDIDASHDDDINKVARVQELIHSYRVRGHLMADTDP
                     LEYRQRRHPDLDVTTHGLTLWDLDRHFATGGFGGVPFLPLRKILGILRDSYCRGIGIE
                     YMHITDPEQREWIQKKVEVGYAKTSRDEQLRILRRLNAAEAFETFLQTKFVGQKRFSL
                     EGGESVIALLDRVLSRAAQDGLDEVCIGMPHRGRLNVLANIAGKSSGQIFREFEGRQD
                     PRSVQGSGDVKYHLGTEGVFTAESGDTTKVYLAANPSHLEAVNPVLEGIARAKQDRIN
                     LGGEDFTVLPILLHGDAAFAGQGVVAETLNLSQLRGYRTGGTVHVVINNQVGFTTAPS
                     SSRSSMYCTDVAKMVQAPIFHVNGDDPEACVRVAELAYEFRKTFQKDVVVDMVCYRRR
                     GHNEGDDPSMTQPLMYKLIEAKRSVRKLYTESLIGRGDISQDEAEAALRDYQQQLEKV
                     FVETKEALKEADKPAEPTLAGTDAEGHSGLEPPTAQTSDATTHSATHTAISVEALQRI
                     GDVHLEAPPGFTVHPKLAQLMERRSAMVREGGIDWGMAEIIAFGSLLMDGTPVRMSGQ
                     DSRRGTFVQRHAVLIDKQNAGEWTPLRYLSDNQAKLWIYDSLLSEFAAMGFEYGYSVE
                     RPDALVLWEAQFGDFVNGAQTIIDEFVSSSEQKWAQRSSVCILLPHGYEGQGPDHSSA
                     RVERFLTLCAEDNMTVTYPSSPANYFHLLRRQAYARPRRPLVVFTPKSMLRLKAASSM
                     PDEFTTGTFRPVMPDRPAATPDKHAVTRVLLASGKVVYELEAEREKRGDTSTAILRLE
                     QLYPLAAAEIAAALTEYPNAELVLVQGEPKNQGAWPFLGLNLPPALAERGYTKPLRVV
                     ARAASASPATGSSKKHAVEQQALLDAAFGR"
     gene            7015..7197
                     /locus_tag="PKU75_03710"
     CDS             7015..7197
                     /locus_tag="PKU75_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HPF80013.1"
                     /translation="MYFTDRGIEELEARRGEEEVTLAWLADQLRTFVDEHPEFEVPVE
                     RLAAWLARLDDPFDDE"
     gene            complement(7246..7611)
                     /locus_tag="PKU75_03715"
     CDS             complement(7246..7611)
                     /locus_tag="PKU75_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HPF80014.1"
                     /translation="MSLRIDPRHSMFLEERPWGRFEQFCVNEPTTVKIITVDPGQRLS
                     LQTHAGRSEFWRVLDGVLDVTVGERTWQAQPGESAWIPVGELHRLGNSGTIPGRILEI
                     AFGDFDESDIERIQDDYTR"
     gene            complement(7726..8904)
                     /locus_tag="PKU75_03720"
     CDS             complement(7726..8904)
                     /locus_tag="PKU75_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPF80015.1"
                     /translation="MPTAEATARAQSEIPAELERDPVFVAHEGGKTAMVATKPLVDRD
                     DLARLYTPGVAKVSSAIAADPHLADAYTARRNTVAVVSDGTAVLGLGDIGPLAALPVM
                     EGKAVLFKQFAGVDAVPLCIETGSVDEIVDAVARLAPSFGGINLEDISAPRCFDIEER
                     LGRRLGIPVFHDDQHGTAIVVLAGLLNAARVLSRDLSSMRMVISGAGAAGVAIAKLLI
                     AVGVEDLIVCDSRGILCRGRVDLTGHKLKLARATNPRRVQGSLADALAGADVFVGVSG
                     GMVPETDIATMALQGIIFALANPNPEVHPEVAGRYAGVVATGRSDYPNQINNVLAFPG
                     IFRGALDSGAADITEPMKLAAAHAIADLVDIPTPSEIVPCVFDVRVAPAVAEAVARHV
                     "
     gene            9023..9379
                     /locus_tag="PKU75_03725"
     CDS             9023..9379
                     /locus_tag="PKU75_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HPF80016.1"
                     /translation="MVSQLRPRLDVVRVTGRSMAPTLRDGDLVLVRRRPPRVGALAVV
                     RLPPDADGMPRPLAIKRVTGRDPGDPQRWWVERDNPREGVDSWLVGSLATADILAVAV
                     LRLWPRPGRLPGPPAG"
     gene            9443..9841
                     /gene="sodN"
                     /locus_tag="PKU75_03730"
     CDS             9443..9841
                     /gene="sodN"
                     /locus_tag="PKU75_03730"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HPF80017.1"
                     /translation="MFRFPFSPTPVSAHCDLPCGVYDPAQARIEAESLKAICKKVAEN
                     DDPDFRTRAVVIKEQRSELVKHHLWVLWTDYFKPPHFEKYPNLHTLFNEATKLAGASG
                     TKGAFDEAKADELLAKIDEIDKIFKETKKA"
     gene            complement(9962..11260)
                     /locus_tag="PKU75_03735"
     CDS             complement(9962..11260)
                     /locus_tag="PKU75_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPF80018.1"
                     /translation="MSGLGRRRAVLAFAVLIVLVGLITWLPSRADLSNLNGFVVSSMV
                     LIVIGELVLVMSHGRQSAPISTATAMGLALAPVGGSGLSLAQCLVAVAVAMTLGALLR
                     AAVGMRTDVFELATRLIGVSVTAACARVEFFGTSLMDWWQQPSTPRWLGGLALIGAAL
                     VGVASDLILWSAGRARTLSVPWAALIHDDATRFGAIGAVMTNAGALIALATPSLGAFA
                     IPLFEWPMVLVLIGLRREERTAATHRQFVRALARLSDETGHTAKGHPRRVARVAVAIG
                     EDLGLSPTELADLRDAALLHDLGQVTLAEPIPDGATVYAAPPAQSWVVQQSVALAAHA
                     GARQPVLDAIAGSAAQFRQQREFGEAIPLVGRIIKVASAYDDLTHGRSKARGPALERI
                     HLGLGYEYDPTVVDALSRVTQDPEPGVSGPLSPTTGPRPR"
     gene            complement(11257..12531)
                     /locus_tag="PKU75_03740"
     CDS             complement(11257..12531)
                     /locus_tag="PKU75_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80019.1"
                     /translation="MTEEASRAYSRSDRTERRYATRTLVVIVVVCVAAVGLCVLTRLL
                     FGPVRSWTNVAILAGVAAATNLPSRLSSRRVNLSLLGVITLAATPLGVGAGIVVIVLA
                     STLVQLGRVPKRTLAFNAGMSTCLGATGVLIYDAIGAPVLPHPTPAGTREVLHVAAGL
                     LLADLVQLLMNAVLIGAIFLASRGVAIRETIGRLLRTGSGAYLASGLLALLIFVAWVP
                     AEGGPLTLLAVGPSLWAARWALNREHEEDTAHLRVVDTLNAAMEIRHPGAGTHAANAA
                     RVAEWLAAEVGTGGVRKLDLALAGALLRLDSLAAEGPDPAPVAGAATQRPARALAGVD
                     FLQHLLPVIEHRDDDPSVQEATQIVVVADAYAEARERLGDDASSRTAAYRSLLAAADG
                     TYDPGVLAALGRLLERVDPIPQPMPTGPGAGS"
     gene            complement(12541..12747)
                     /locus_tag="PKU75_03745"
     CDS             complement(12541..12747)
                     /locus_tag="PKU75_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80020.1"
                     /translation="MSSSRALRVVAAAAIAFAVPAGAAHAGSAGNASGGGSQHTTQAS
                     QQGDQISQDQCKDHWEWRRDCRNG"
     gene            complement(13122..13385)
                     /gene="rsrA"
                     /locus_tag="PKU75_03750"
     CDS             complement(13122..13385)
                     /gene="rsrA"
                     /locus_tag="PKU75_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HPF80021.1"
                     /translation="MNAELNETDCSEALHRVFEYLDGELEPAERAKIAAHLAHCAACL
                     QQYDLDTMVKALVKRSCAQEAAPAHLRGMIVQQITSIEVRYLD"
     gene            complement(13382..14044)
                     /locus_tag="PKU75_03755"
     CDS             complement(13382..14044)
                     /locus_tag="PKU75_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF80022.1"
                     /translation="MTATPPTDELAGDALAADAGTVDVSAEAPEERRARFEELALPLL
                     DQLYSAALRTTRNPADAEDLVQETYAKAFAAFHQFKPGTNLKAWMYRILTNTYINSYR
                     KKQREPIQSDAAEIEDYQLARAESHTSSGLRSAEIEALDHLPDSEVKRALQELPEEFR
                     LAVYFADVEGYAYKEIAEIMGTPIGTVMSRLHRGRRLLRELLTEYAAERGFGTKGGGD
                     QE"
     gene            14224..16335
                     /gene="malQ"
                     /locus_tag="PKU75_03760"
     CDS             14224..16335
                     /gene="malQ"
                     /locus_tag="PKU75_03760"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPF80023.1"
                     /translation="MTAPNTDLADLAKAYGVATDFWDWQGVHTIVSAETITRVLTALG
                     VDVTSPEALAQARDEVWRSSWRRTLPPTIVSREGWTPWVPVHLPHGAAVRVELALEDG
                     GTIAVPQVDNWVEPRLVDGVLTGEATFQLPAEVPLGWHLLWAHVEGREPAYATVVVTP
                     QRLELPAGLEHRRMTGLMSQVYQLRSANSYGVGDLADLADLATWGAADLGCDWVLVNP
                     LHAAQACPPMEASPYLPTTRRFVNPLYIRVEDIPEVAYLSVEVRAAVAAQARTGQALN
                     TSETIDRNASWAAKRAALELVYAAGLSPRRARAFEGFCEREGQGLVDFATWCALADMH
                     GEDWRSWPAGLQSPTSPQVDEFRAEQAAEIDFFRWLQWVLEEQLAATQRLAKEAGMAL
                     GVIHDLAVGVHGSGADSWGLGDALARGIEVGAPPDQFNQIGQNWSQPPWRPDRLAELG
                     YAPYRDMLRTVLRDSGGIRVDHIIGLFRLWWIPQGMSPGEGTYVHYDHEALIGILCLE
                     AHRAGAVVVGEDLGVVQPFARDFMKERGILGTSILWFEWRDGKALAPEGYRELCLASV
                     TTHDLAPTAGYLELVHVALREKLGLLTRDAAVEEAEERAAIARVAEALEARGLPHDPS
                     DVEEFVISLHRYLALSPAKLLGISVPDLIGDRRVVNQPGTDKEYPNWCVPLTGPSGDL
                     VSLEQLTALPYPRRLARSVTG"
     gene            complement(16364..17116)
                     /locus_tag="PKU75_03765"
     CDS             complement(16364..17116)
                     /locus_tag="PKU75_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HPF80024.1"
                     /translation="MRDNATPSETATAASPHEGEPTQHVANTASQLNWLRAGVLGAND
                     GIVSTAGLVIGVAAATANRGAIFTAGLAGLAAGAMSMAVGEYVSVSTQRDTEKAMLAK
                     ERRELHEKPQDELDELAGIYESKGLQPDLAREVAVALTHHDALTAHAEAELGIDPSEL
                     TNPWHAAFASMVSFTLGALLPLIAVLNPSHTWRLPGTVVTVLVALALTGYVSARLGGA
                     RIGRAVARVVAGGIIAMAVTYAIGRLFGTAIG"
     gene            complement(17151..18311)
                     /locus_tag="PKU75_03770"
     CDS             complement(17151..18311)
                     /locus_tag="PKU75_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80025.1"
                     /translation="MTAAAEQTHAQPGDVLVDVQGIKVHFPIKKGVIFDKTIGYVYAV
                     DGVDLQIRRGETYGLVGESGCGKSTLGRAILNLEPPTEGKVVFDGTDIASLKGEALRR
                     KRQHMQMVFQDPLSSLDPRQSVESLLLEGMKAHGLVKDTKAANARLKELLKAVGLPAA
                     SLQKYPHEFSGGQRQRIGIARALAVDPQLIVADEPVSALDVSVQAQVINLLEDLQDRF
                     GLTYLVVAHDLAVVRHISDRVGVMYLGALVEEAAADDLYAAPMHPYTRALMSAVPVPD
                     PVVEDRRERILLNGDLPSPANPPKGCRFHTRCPWRQPTQCDTDRPALRVVSIDGVPET
                     HRVACHFAEGIASGELQPHKVEAEVVDPHLGESDAHGSLIGPATTVEVTDTP"
     gene            complement(18308..19363)
                     /locus_tag="PKU75_03775"
     CDS             complement(18308..19363)
                     /locus_tag="PKU75_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80026.1"
                     /translation="MTTRAYAADTRSGEALLSVRDLSVVFQRHGEQPFAAVDGVSFEV
                     RPGQTVGLVGESGSGKSVTSLAIMGLLPARGNRVTGVADFDGTDLLKLSSGDMRDRRG
                     KDLGMIFQDPLSSLNPVVPIGVQVTEVLERHKGMSRKAAMPKAREMLDKVGIPDPDRR
                     LKDYPHQLSGGMRQRALIAMALACEPRLLIADEPTTALDVTIQAQILQLLGDLVRDSG
                     TALIMITHDLGVVAGLCDEVNVMYGGRIVERGLRHDLFAAPRHPYTHGLLGSIPRLDG
                     QRGEPLIPIPGSVADNLPWDHACAFAPRCFQPIDVCRSQTPGFVEDGASALRCHNPVG
                     SPHGHTAQPDHAEVNDQ"
     gene            complement(19369..20352)
                     /locus_tag="PKU75_03780"
     CDS             complement(19369..20352)
                     /locus_tag="PKU75_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF80027.1"
                     /translation="MTDNPKGWKPGARRKAKIDALAQAAGQVQDAGTVADTPGVSLIA
                     SAWRRLRRDRVFLIGLTITVLFLLVALFAPLLAPHDPTARLLLDKVRPQSNPIPGPEP
                     GFPLGADNVGRDLLSRLIVGSRQTLLVCVFATLFSLIGGLILGTLAGAFGGWIDSVVM
                     RFVDIMLSIPSLLLAFSVAAMAARPSQWTVILAISIAYLPIFARLLRGSMLAQRHSDH
                     VLAARALGVKQAPIIFRHMLPNSLGPVIVQATLVLATAVIDAAALSFLGLGNPDDRQP
                     EWGQMLGAAQKYIYDYPHLAFYPAACIILVAFGFTLMGESLREALDPKSRR"
     gene            complement(20360..21367)
                     /locus_tag="PKU75_03785"
     CDS             complement(20360..21367)
                     /locus_tag="PKU75_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF80028.1"
                     /translation="MLRFIVRRLLQMVGVLFVLSLLLFIWLRMLPGGTVSAILGDRAT
                     PESRERLTKALGLDEPLYVQYWRYLKRALTGNFGASTGVVPGRDAFQIFIDRFPATIE
                     LSFFAILIAILLGIPLGYLAARRRASLFDNASVIVSLIGVAVPVFFLAFLLKYFFAVK
                     LGWLPVSGRQSNIDATRVTNFFVVDGLMTREWDAAWDAFKHLILPAITLSTIPFAVIF
                     RITRASVLEVLDEDFVRTAESKGLTNRIIRGRHVLRNALLPVITTVGLQVGGLLAGAI
                     LTESVFSFPGIGEALASAFSHRDYAVLQVLIMASAATFVIVNLIVDVLYAVVDPRVRT
                     R"
     gene            complement(21468..>23047)
                     /locus_tag="PKU75_03790"
     CDS             complement(21468..>23047)
                     /locus_tag="PKU75_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPF80029.1"
                     /translation="GTAAAEGGSDTFTFGAAGAPEVFDPYYASDGETFRITRQIFQGL
                     LGIKPGTADAQPELAESWEPSDDGLTWTFKLRQGVKFTDGEAFNAAAVCYNFERMFEQ
                     NEVAQAGPAEYWAYTMLGFKGGETESLYKSCEAKDDATAVITLNRYTSDFPVMLTLES
                     FSMQSPKALKDGDANNVQKEGEGFKYPAYASAPVGTGPYKLEKYDEANKTITLVRNDD
                     FWGEKAKTANIVFKIIPDESTRRQELEAGSINAYDLPNPADWKGLEDAGNQVLVRPAF
                     NILYMGLNPEKNAKLQDVRVRKALYMALNRDQLVATQLPEGAKVATQFMPDTVAGYNT
                     GLEATKYDVEGAKALLKEAGAEGMTLQFAYPSEVSRPYMPDPQKIWDALSADLEAIGV
                     KVEAVTKPWNGGYLDGVDNGLFDAWLLGWTGDYNSANNFIGTFFGADSNDFHTWSTDF
                     GKTLSKELDETNGIVDEGERTTAYEALNQKIMEDYLPGLPISHSPPALVVGKGVEGLT
                     ASPLTAEMFDTVTVSKS"
     assembly_gap    23048..23057
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23329..>23987)
                     /locus_tag="PKU75_03795"
     CDS             complement(23329..>23987)
                     /locus_tag="PKU75_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPF80030.1"
                     /translation="AAAVAGLAALLAFALLTVAVLAPWAALIAADREVSTPAMRFADD
                     AGLRGLLVAWQAASMPSCAHGALTVIAALAWWRHRQTRRVLLALAAAYLTWWLTPILK
                     NAVDRPRPQVPDPLWGIGGYSFPSGHASNVTAAALALGIVLWPVMPASGRRWVAAGGG
                     AVVVLTCADRVLLGVHYLTDVLAGVLLGAGIGCLALAGTRFHRITNREALPAGIPRAA
                     "
BASE COUNT         3843 a         8248 c         8325 g         3561 t
ORIGIN      
        1 ccgacgatct cctcgacgac gtcttcgagg gtgacgacgc ccgcggtgcc gccgtactcg
       61 tccacgacga cggcgaactg cgggcccgac tgtcgcaaca ggtcgagcag ggggtcgagc
      121 cgcagcgtct ccggcaccac gaccggcggc gtcatcagcg ccgacaccgg caccgccgcc
      181 cgccgcccgc gcggcaccga gatggccgcc ttgacgtgca cgatcccgtc cacatcgtcc
      241 cagtcgccgg cggtgacggg gaagcgggag tgcccggtct gccgggccaa ctccacgaca
      301 tcggcggcgc tcgcggtgcg ctcgatgttc cacgcccggc cacgcggcgt catgacgtcc
      361 tcggccgtct gcgccccgaa acgcagcgac gccgccacca gccgccccgt gccctcatcc
      421 agcgtcccgg cctggaccga gcgacccacg agcgaggcca gctcctgcgg cgtccgggcc
      481 gccgacaact cctcctgcgg ctcgacgccc acggcccgca ggaaggcgtt ggccgagccg
      541 ttgaggacgg cgatcagcgg cttggccacg agcgcgaaca cccgcgtcgg cccagcgacc
      601 ttcttgccca cccgcagcgg cgcgctgatg ccgaggaact gcggcaccaa ctcgccgaac
      661 accatcgaga acaaggtggc tagcagaagc gcgaccgcgc tgctgaccgt gtccagcgcc
      721 ccctccggca ccccgaccgc atcgatcgcc ggccgcagaa tggtcgcgat cgacggctcg
      781 gcgatgaagc ccagcacgag ggtggtcagg gtgatcccca cctgcgcggc cgacagctgg
      841 gtcgacaggc cacgcaacga cgtgagcacc gtctgcgcct cgtgatcgcc ttggtcgacc
      901 gcccgctgca ccgtcggccg gtcgagcgcg acgagcgaga actcggtcgc gacgaagagg
      961 gcggtgccca cggtcaggac gatcgccgcg agcagcagca gccagggcac catagtggcg
     1021 tcatcgtaat ggcccgcccg ccacgcaacc gtgctcacgc gacgttcacc ggcacctgat
     1081 tgaatgcccc cgacaggaga acgccagcag gaggggaacc gcgaatgagc gtggcggcaa
     1141 caacgacggg cacgtcgatc gagatcgacg gcctgagcaa acgtttcggc gatttcctcg
     1201 ccgtcgacaa cctcaccttc cgggtccagc cgggtcgggt caccggcttc ctcggcccca
     1261 acggggccgg aaagaccacg acgctgcgta tgctgctcgg cctggtccgt cccaccggtg
     1321 gcacggcgac gatcggcggg cgtgcctacc agacgatccc cgagccgatc cgcacggtcg
     1381 gcgcggccct ggaggccacc aacttccacc ccggccgcac cggccgcgac cacctgcgcg
     1441 tgcttgccgc cacggccggc atacccgaca cccgggtcga cgaactgctc gagctggtcg
     1501 gcatacccgc ggcggcccgc aaacgcgccg gcggctactc gatgggcatg cgacagcggc
     1561 tcggcctcgc cgtcgccctg ctgggcgacc cgcacgtcct cgtcctcgac gagcccgcca
     1621 acggcctgga ccctgaaggc atccggtggc tgcgcggctt catgcgccac ctcgccggcc
     1681 agggcaagac gatcctcgtc tccagccaca tgctccaaga ggtggagcag accgtcgacg
     1741 acgtcgtcat catctccaac ggtcgcctga tccgctccgg cacgatggcc gaactccacg
     1801 gcccggcaac ctcgctcgtg cggacctcgg acccgggcgc gctggccggt gccctgcggg
     1861 tcgccgacgt caccagtcat accgccgacg acggcggcct gatcgtcgag accgacgacc
     1921 tgcgcctcat cggtgacgtc gcgctgcgcg ccggactgcc cctatggcag ttgcagccgc
     1981 aggtcagcga cctcgaagac atgttcttcg ccctcaccga gggccagaac cgcaacctcg
     2041 gcgccaccga tgccgccgct ccacacgaaa tggacgccat cgccggacgc gacggaggga
     2101 acgagaagtg atcggggcca tcagatccga gatccgcaag gtcttcacca cccggctgtg
     2161 gtggggcatg ggcctgggta tggccgtgct ggccgccctg atctccatgg gtttcgccgc
     2221 cctgctcggc aacgccgcga tgaccggcgg cgacaacaac ggccagggca atcccttcgc
     2281 gaacatgacc gtcgggaccg ctcagctcat ctacaacgcc ggcatcatcc agcagatgac
     2341 gatcctcttc ccgctagcgc tcggcgtgct gctcatcacc agcgagtacc ggcacaagac
     2401 gatctccgcc acgttcttgt ccacccccaa ccggtgggtg gtcctgctct cgaagatggt
     2461 cgcggtcgcg gtcaccgggg cgctgtatgc cgtggtgcac gccatcgcgt ccgtcgccgg
     2521 tggcgcctcg atcatcaagt tcgtgaagaa ccagccgacg atgctgggcg agacggaggt
     2581 ctggaactcc ctcggcatcg ggctgatcgc cttcgcgatc tggatgctgt tcggtttcgg
     2641 ggtcggcatg ctgatccgca accagatcgc cgcggtgctg ctggccgtgg gcgccacctt
     2701 cgtggtccag atcgccttga acatcatctt ctccgtcaag gagtggtact ccgcgatgaa
     2761 gtggatccca ggcaatctca ccgccaatat ggtcatcacg agtgacccca ccgccgggca
     2821 gacggtggat ccggcgacgc agtcccaata cttcgcccac tggtggcagg cgggtctggt
     2881 cctggctgcg tatgccgccg tgctcgccgt cgtcggagcc tggctcacca cgcgccgcga
     2941 cgtcgcctga ggccgcggcc gggtcccgac gggtcgctgc ccgtcgcacc atgaccaccg
     3001 ctcgccccgc cgttctcgct cctgggacgg cggggccatc gggcgaggga gcggggtaac
     3061 ctggtgaggt tcgttttttg tccgtcaccg atgacgatgt gaaagaggcg tgcggccgtg
     3121 gctgacccga cccccgcgaa tcccatggag gccttcggcc cgaatgaatg gctcgtcgac
     3181 gagctctacg agagctacct taaggatcgc aactccgtcg accgtgcctg gtgggatttc
     3241 ttctcgacct acaagctcga gcacccgcac ggcgccggtg gcaacggcac gcagcccgcc
     3301 gccccggcgg cccgcacgtc gaccgcgccg caggccccca agtccgcccc gccggcgccg
     3361 accaccaacc cggcggccac cgagaacccc cagtccacga ccgccgagac cggcccgacg
     3421 cgtcccggtg ccagcggccc gggctcggcg gcgcaggccc ccgtcccggc cgatgccccg
     3481 gcacccaagc cggagatgcg cgccccggaa cggcccaccc cgacggggcc gaacgccacc
     3541 ccgcgtgatg ccgccaagcc cgccgccgcc aaggccaccg aagagcccac cgtctccccc
     3601 atccgcggtg ccagcgcccg ggtcgtcacc aatatggagg cctcgctcga gatcccgacg
     3661 gcgacgagtg tccgcagcgt cccggcaaag ctgctgatgg acaaccggat cgtcatcaac
     3721 aaccacctgc ggcgcacccg cggcggcaag gtgtccttca cccacatcat cggctacgcc
     3781 ctggtcaagg ccctggcgca gatgccggag atgaacaaca gcttcaccga ggagaacggc
     3841 aagcccgcgc tctcggtccc cgcgcacgtc aacctcggca tcgccatcga tctggccaag
     3901 ccagacggca cccggcagct gctcgtgccc tccatcaagg ccgccgagac gatggacttc
     3961 ggccggttct ggtcgagcta cgaggacatc atcaagaagg cccgcggcgg caaactcgtc
     4021 gtcgaggact tcatgggcac gacgatctcg ctgaccaacc ccggcacgat cggcaccgtc
     4081 ctctcggtgc cgcggctcat gaagggccag ggcgccatca tcggtgtcgg cgcgctcgaa
     4141 tacccggccg aatggcaggg cgcgagcacc accgtcctcc accgccatgg cgtcagcaag
     4201 atcctgaccc tcacctcgac atacgaccac cgcatcatcc agggcgccca gtcgggcgac
     4261 ctgttgcgga tcatccacaa cctcctgctc gggcaggacg gcttctacga cgagatcttc
     4321 gagtcggtcc ggatccccta cgagccggtc cgctgggtcc aggacatcga cgcctcccac
     4381 gacgacgaca tcaacaaggt cgcgcgggtc caggaactca tccactccta ccgggtgcgc
     4441 ggccacctga tggccgacac cgacccgctt gaataccgcc agcggcgcca ccccgacctc
     4501 gacgtcacca cgcacggcct gaccttgtgg gacctggacc ggcacttcgc gaccggcggc
     4561 ttcggcggcg ttcccttcct gccgttgcgc aagattctcg gcatcctgcg tgactcctac
     4621 tgccgcggca tcggcatcga gtacatgcac atcaccgatc ccgagcagcg cgagtggatc
     4681 cagaagaagg tcgaggtcgg ctacgccaag acctcccgcg acgagcagct gcggatcctg
     4741 cgccgcctca acgccgcgga ggccttcgag accttcctgc agaccaagtt cgtcgggcag
     4801 aagcgcttct ccctcgaggg cggcgagtcc gtcatcgcct tgctcgaccg cgtgctgtcg
     4861 cgggccgcgc aggacggtct ggacgaggtg tgcatcggta tgccgcaccg cggccgcctc
     4921 aacgtgctcg ccaatatcgc gggcaagtcc tccgggcaga tcttccgcga gttcgagggc
     4981 cggcaggacc cccggtcggt gcagggctcg ggcgatgtga agtaccacct cggcaccgag
     5041 ggcgtcttca ccgccgaatc gggcgacacc accaaggtct acctggccgc caacccctcc
     5101 cacctcgagg ccgtcaaccc ggtgctggag ggcatcgccc gcgccaagca ggaccgcatc
     5161 aacctcggcg gcgaggactt cacggtgctg ccgatcctgc tgcacggtga tgcggccttc
     5221 gccggccagg gcgtggtcgc cgagaccctc aacctctcgc agctgcgcgg ctaccgcacc
     5281 ggcggcaccg tgcacgtcgt catcaacaac caggtcggct tcaccaccgc gccgagcagt
     5341 tcgcgcagct cgatgtactg cacggacgtc gccaagatgg tgcaggcgcc gatcttccac
     5401 gtcaacggtg acgaccccga ggcctgcgtg cgggtggccg aactcgccta cgagttccgc
     5461 aagaccttcc agaaggacgt cgtcgtcgac atggtgtgct accgccgccg cggacacaac
     5521 gagggcgacg acccgtcgat gacgcagccg ctgatgtaca agctcatcga ggccaagcgc
     5581 agcgtccgca agctctacac cgagtccctc atcggccgtg gcgacatctc ccaggacgag
     5641 gccgaggccg cgttgcggga ttaccagcag cagctggaga aggtcttcgt cgagaccaag
     5701 gaggcgctca aggaggcgga caagccggcc gagcccaccc tcgccggcac cgacgccgag
     5761 ggccactccg ggctcgaacc tccgaccgcc cagacctccg acgcgacgac ccactccgcg
     5821 acgcataccg ccatctccgt cgaggcgctg cagcggatcg gcgacgtcca cttggaggct
     5881 ccgcccgggt tcaccgtcca ccccaagctc gcgcagctca tggagcgccg ttcggcgatg
     5941 gtccgcgagg gcggcatcga ctggggcatg gccgagatca tcgccttcgg gtccctcctc
     6001 atggacggca ccccggtgcg gatgtccggc caggacagcc gccgcggaac cttcgtgcag
     6061 cggcacgcgg tgctgatcga caagcagaac gccggcgagt ggaccccgct gcgctacctg
     6121 agcgacaacc aggccaagct gtggatctac gactcgctgc tgagtgagtt cgccgcgatg
     6181 ggcttcgaat acggctactc cgtcgaacgg cccgacgccc tcgtgctgtg ggaagcccag
     6241 ttcggcgact tcgtcaacgg cgcccagacg atcatcgacg aattcgtctc ctcctccgag
     6301 cagaagtggg cccagcgcag ttccgtctgc atcctgctgc cgcacggcta cgagggccag
     6361 ggcccggacc actcgtcggc acgggtcgag cgtttcctga cgctgtgcgc cgaggacaat
     6421 atgacggtca cctacccgag ttcgccggcg aactacttcc acctgctgcg ccgccaggcc
     6481 tatgcccggc cgcgccggcc gttggtcgtg ttcaccccga agtcgatgct gcgtctgaag
     6541 gccgccagct cgatgccgga cgagttcacg accggcacct tccggccggt catgccggac
     6601 cgccccgcgg ccacgcccga caagcacgcc gtcacccggg tgctgctcgc ctcgggcaag
     6661 gtcgtctacg agttggaggc cgagcgcgag aagcgcggcg acaccagcac ggccatcctg
     6721 cggctggagc agctgtatcc gctggccgct gccgagatcg ccgcggcgct cacggagtac
     6781 ccgaacgccg agctggtgct cgtgcagggc gagccgaaga accagggcgc gtggcctttc
     6841 ctcggtctca acctgccgcc ggcgctggcc gagcggggct acaccaagcc gctgcgggtg
     6901 gtcgcccggg ccgcatcggc gtcgccggcg accggcagct ccaagaagca cgccgtcgag
     6961 cagcaggcgc tgctcgacgc cgccttcggg cgctgaggtc cgaacgccgg ccgcatgtac
     7021 ttcaccgatc ggggcatcga ggaactggag gcccgccggg gcgaggaaga ggtcaccctc
     7081 gcctggctgg ccgaccagtt gcgcaccttc gtcgacgagc acccggagtt cgaggtgccg
     7141 gtggagcgcc tcgccgcctg gctggcgcgc ctcgacgacc ccttcgacga cgaataggcc
     7201 ggctcgaatt cgccgccggc agataataca gcgaaatccg ccggctcagc gggtgtagtc
     7261 gtcctggatg cgttcgatat ccgactcgtc gaaatcgccg aaagcgatct ccaggatccg
     7321 gccgggaatg gtgccggaat tgccgaggcg gtgcaattca cccaccggga tccacgccga
     7381 ttcgcccggc tgagcctgcc aggtccgctc gcccacggtc acatcgagca ccccgtcgag
     7441 gacccgccag aactcggaac ggccggcgtg ggtctgcagg gacaaccgct ggcccgggtc
     7501 gaccgtgatg atcttgacgg tcgtgggctc gttgacacag aactgctcga agcgccccca
     7561 cggtcgttct tcgaggaaca tcgagtgccg cggatcgatc ctcagactca tgacaacctt
     7621 tgcgatggcg gcgtgaccgg tgaaatgcca gcggaaaagg acccgcggtg gtggcctgcg
     7681 attctatcag ccggcccatg acggtcagtc ccggccgagg acctgtcaga cgtgccgggc
     7741 caccgcctcc gcgaccgccg gagccactcg cacatcgaag acgcacggca cgatctccga
     7801 cggcgtcggg atgtcgacga ggtcggcgat cgcatgggct gcggccagct tcatcggctc
     7861 ggtgatatct gcggcaccac tgtccaaggc tccccggaag atccccggga acgcgaggac
     7921 attgttgatc tgattcggat aatccgaacg ccccgtggcg acgaccccgg cataccggcc
     7981 ggcgacttcg gggtgcactt ccggattcgg attcgcgagg gcgaaaatga tcccctgcag
     8041 cgccatcgtc gcgatatccg tctcgggcac cataccgccg ctgacaccga cgaagacatc
     8101 ggcgccggcg agggcatcgg cgagcgaacc ctggacgcgg cgggggttcg tggcccgggc
     8161 cagcttgagt ttgtgaccgg tcaggtcgac gcgcccccgg cacaggatgc cgcgcgagtc
     8221 gcacacgatc aggtcctcca caccgaccgc gatgagcagc ttggcgatgg ccacacccgc
     8281 ggctccggct ccgctgatca ccatgcgcat gctcgacagg tcccggctga gcactcgcgc
     8341 ggcgttgagc agcccggcga gcacgacgat ggccgtgccg tgctgatcgt catggaagac
     8401 cgggatcccg aggcggcggc ccaggcgttc ctcgatgtcg aaacaccggg gcgccgaaat
     8461 gtcctccaag ttgatgccgc cgaagctcgg cgccagccgc gccacggcat cgacgatctc
     8521 gtcgaccgag ccggtctcga tgcacagcgg cacggcgtcg acaccggcga actgcttgaa
     8581 gagcaccgcc ttgccctcca tgacgggcaa cgccgccagc gggccgatgt ccccgagccc
     8641 gagcaccgcg gtgccgtcgc tgacgacggc gaccgtgttg cgccgggcgg tataggcatc
     8701 ggccaggtgc gggtccgccg cgatggccga actcaccttg gcgaccccag gcgtgtagag
     8761 ccgcgccaga tcgtcgcggt cgaccagcgg cttggtcgcc accatcgcgg tctttccacc
     8821 ctcgtgggcg acgaagacgg gatcgcgttc cagctcggcg gggatctcgg actgggcgcg
     8881 cgcggtcgcc tcggcggtgg gcatcgactt cctcttcctt cggtcctacg cccgcggcgc
     8941 gggcggcgcc gacgggtggc gttcgggcag aggtggggcc cagtgacaag cctgccgccg
     9001 gcggtggccg tacggttgga gcatcgtttc acaacttcgc ccccgtcttg atgttgtccg
     9061 cgtcacgggc cgttcgatgg ccccgacgct gcgcgacggc gacctggttc tggtgcggcg
     9121 ccgtccgccg cgcgtcggcg ccctcgccgt cgtgcgtctg cccccggacg ccgacggtat
     9181 gccgcgaccc ctcgcgatca aacgcgtcac cggccgcgac ccgggggatc cgcagcggtg
     9241 gtgggtggag cgggacaatc cgcgcgaggg agtggacagt tggctggtcg gctccctcgc
     9301 gacggcggac atcctggccg tggcggtcct gcggctttgg ccgcgcccag gacgcctgcc
     9361 cggacctcca gcggggtagg ttggacgaag cgaccatccc agccgtggcc gcaatgccat
     9421 cgacgagaga ggttccaccc cgatgttccg cttccccttc tcccccaccc cggtcagcgc
     9481 gcactgcgac ctgccgtgcg gtgtctatga cccggcgcag gcccggatcg aggccgagtc
     9541 gctcaaggcg atctgcaaga aggtcgccga gaacgacgac cccgacttcc gcacccgcgc
     9601 ggtcgtaatc aaggagcagc gctcggagtt ggtcaagcac cacctgtggg tgctgtggac
     9661 cgactacttc aagcccccgc acttcgagaa gtaccccaac ctgcacacgc tgttcaacga
     9721 ggccaccaaa ctggccggcg caagcggcac caagggcgcc ttcgacgagg ccaaggcgga
     9781 cgagttgctc gcgaagatcg acgagatcga caagatcttc aaggagacca agaaggcctg
     9841 agccttcgcg tcacctgccg cggcgggcgg cccccacacg tcgggggccg cccgccgctg
     9901 tcgtgcagct gggatgtgtg cgctggggtg tgtgcgctgg ggggttggcg gtgggcggag
     9961 gtcagcgcgg acgcgggccg gtcgtcgggc tcagcgggcc gctcacgccg ggttcggggt
    10021 cctgcgtgac ccgcgagagc gcgtcgacga ccgtcgggtc gtactcgtag cccagcccga
    10081 ggtggatccg ctccagcgcg gggccgcgtg ccttgctgcg gccgtgggtc aggtcgtcat
    10141 aggcactggc gaccttgatg atccgcccga cgagggggat ggcctcgccg aactctcgct
    10201 gctgacggaa ctgagcggcg ctgccggcga tcgcgtccag caccggctgt cgcgcgccgg
    10261 cgtgcgcggc gagcgcgacg gactgctgga cgacccaact ctgggcgggc ggggcggcat
    10321 acaccgtcgc cccgtcgggg atgggctcgg ccagggtcac ctgaccgaga tcgtgcaaca
    10381 gtgcggcatc gcgcaggtcg gccagctcgg tcggcgatag gccgaggtcc tcaccgatcg
    10441 cgaccgccac ccgcgcaacg cgccgcgggt ggcccttggc cgtgtgcccg gtctcgtcgc
    10501 tgagacgcgc cagcgcccgg acgaactggc ggtgcgtcgc agcagtgcgc tcctcccggc
    10561 gcagcccgat gagcaccagc accatcggcc attcgaagag cgggatggcg aacgccccaa
    10621 gggagggggt ggccagcgcg atgagagccc cggcgttcgt catcacggca ccgatcgcgc
    10681 cgaaccgtgt ggcgtcgtcg tgaatcaggg ccgcccacgg caccgacagg gtgcgggccc
    10741 gtccggcact ccacaggatg agatcacttg ccaccccgac gagggcggcc ccgatgagag
    10801 cgaggccacc gagccatcta ggggtgctcg gttgctgcca ccagtccatg agcgaggtgc
    10861 cgaagaactc gacgcgggcg caggccgccg tcacgctcac gccgatgagc cgcgtcgcca
    10921 gctcgaagac atcggtgcgc atgccgaccg cggcccgcag cagcgccccc agggtcatgg
    10981 cgacggcaac cgcgacgagg cactgggcaa gggagagccc ggacccgccg acgggcgcga
    11041 gcgcgagtcc catcgccgtt gcggtgctga tgggcgcgct ctggcgcccg tgcgacatga
    11101 ccaggaccag ctccccgatc acgatcaaca ccatcgacga cacgacgaac ccgttgaggt
    11161 tgctgaggtc ggcgcgcgac ggcagccagg tgatcagacc gacgaggacg atcaggaccg
    11221 cgaacgccaa gaccgcgcgc cgtcggccca ggccgctcat gagccggctc cgggaccggt
    11281 gggcatcggc tgcgggatcg ggtcgacgcg ctcgagcagg cgtccgagtg cggcgagcac
    11341 gccggggtcg taggtgccgt ccgccgccgc cagcaagctg cggtatgccg ccgtccggga
    11401 gctcgcatcg tcgcccagac gttcgcgggc ctcggcatac gcgtccgcga ccacgacgat
    11461 ctgggtcgcc tcctgcacgg acgggtcgtc gtcacggtgt tcgatcaccg gcagcagatg
    11521 ctggaggaag tcgactcccg cgagcgcccg cgcgggtcgc tgcgtcgccg cgccggcaac
    11581 cggggcaggg tcgggcccct cggccgccag ggaatccagc cgcagcaagg cgccggccag
    11641 ggccaggtcc agcttgcgca cgcccccggt gccgacttcg gccgccagcc actcggccac
    11701 ccgagccgcg ttggcggcat gggtgcccgc gccgggatgg cggatctcca tggcagcgtt
    11761 gagggtgtcg acgacgcgca gatgcgccgt gtcctcctcg tgttcgcggt tgagcgccca
    11821 gcgcgcggcc catagcgacg ggcccacggc gagcagggtc agtggcccgc cctccgcggg
    11881 cacccaggcg acgaagatca ggagcgcgag cagcccggac gcaaggtagg cgccgctgcc
    11941 ggtgcgcagc agccggccga tcgtctcgcg gatggcaacg cctctggagg ccaagaagat
    12001 ggcgccgatg aggacggcat tcatcagcaa ctggacgagg tcggccagca gcagcccggc
    12061 ggcgacgtgc aacacctcgc gggtgccggc gggtgtggga tggggcagca cgggggcacc
    12121 gatggcgtcg tagatcagga caccggtcgc gcccaggcag gtcgacattc cggcgttgaa
    12181 cgccaacgtc ctcttcggga cgcggccgag ctggacgagg gtggacgcga ggacgatgac
    12241 cacgatgccg gcgccgaccc cgaggggggt ggcagccagc gtgatcacgc cgaggaggga
    12301 gaggttgacg cgacgggagg acaggcgcga cggcaggttg gtcgcggcgg cgacgcccgc
    12361 gaggatggcg acattggtcc agctgcggac cggaccgaac agcagacgcg tcaggacaca
    12421 cagccccacg gccgcaacgc acacgacgac gatgacgacg agtgttcgcg ttgcgtatcg
    12481 gcgttcggtc cgatcggacc gtgaataggc gcgggatgcc tcttcagtca ccgggctccg
    12541 tcagccgttg cggcagtcgc ggcgccactc ccaatggtcc ttgcactggt cttggctgat
    12601 ctggtcgccc tgctgggaag cctgagtggt gtgttgcgag ccaccgccgg aggcgttgcc
    12661 cgccgagccg gcgtgggcgg cgccggccgg gacggcgaag gcgatggcgg ccgcggcgac
    12721 gacacgaagc gcacgtgaac tggacatttt acgactccta tcagggaaga ttctacgcgg
    12781 cccgttccgc tccggaccgc ctttagccgg aattgtgtgg catggcccag acgaacgcaa
    12841 tcgtcctgct ccgcctgctg ggcgcacctc ggatcggatg gggcacgtgt tgcgatatgg
    12901 gacaatgact acgtacgtcg gtatcccctg cggagccgaa accccggacc tggagttgtc
    12961 tcatgagcaa gcgtgcccgc aagcgccgcg accgtaagaa gaagggcgcc aaccacggcc
    13021 gcaagccgaa cgcctgaccc accgcacaga gaccggggtc ccgccagcgc gggaccccgg
    13081 tctatgtgtg cgtaaaagcg gtgccgcgta cctagctgcg gctagtcgag gtagcggacc
    13141 tcgatgctcg tgatctgttg cacgatcatc cctcgcaggt gggccggagc cgcctcctgg
    13201 gcacaggatc gcttgacgag ggccttgacc atcgtgtcga ggtcgtactg ctgcaggcac
    13261 gcggcacaat gcgcgaggtg agcggcgatc ttggcgcgct cggctggctc cagctcgccg
    13321 tcgaggtatt cgaagacccg gtgtagggcc tcggaacagt cggtctcgtt gagttcggcg
    13381 ttcactcctg gtcaccgccg cccttggtgc cgaagccgcg ctcggcggca tactccgtga
    13441 gcaactcacg cagcaggcgg cggccccggt gcaggcggga catcaccgtg ccgatgggcg
    13501 ttcccatgat ctcggcgatc tctttgtagg cgtagccctc gacgtcggcg aagtagaccg
    13561 cgaggcggaa ctcctcgggc aactcctgca gggcgcgctt gacctcgctg tcgggcaggt
    13621 ggtcgagcgc ctcgatctcg gcgctgcgca acccgctgga ggtgtgggac tcggcgcggg
    13681 cgagttggta gtcctcgatc tccgccgcat cggactggat cggctcgcgc tgcttcttgc
    13741 ggtagctgtt gatataggtg ttggtgagga tgcgatacat ccacgccttg aggttggtgc
    13801 ccggcttgaa ctgatgaaag gccgcgaacg ccttcgcgta ggtctcctgg accaggtctt
    13861 cggcgtcggc cgggttgcgg gtggttcgca gggccgcgga atatagctgg tcgagcagcg
    13921 gcagcgcgag ttcctcgaaa cgagcccggc gttcctcggg cgcctcggcg ctcacgtcga
    13981 cggtcccggc atccgcggcc aaggcgtcgc cggccagttc gtcggtaggc ggagtagcag
    14041 tcatcgcgga ccaccctaac gcgggcatcg ccggcgacgg tgtttgcagg acgagcacaa
    14101 gacctccgga agcgggcgtc aggaatttct caaaacaacg aacgccgtgg cggggctatt
    14161 ccgtgcggcg tgggacacta ggtcgccccc gctcgacgac gccccgaagg agctgcgccg
    14221 cccatgaccg cgcccaacac cgatctcgcc gacctcgcga aggcctacgg ggtcgccacg
    14281 gacttctggg actggcaggg cgtgcacacg atcgtcagcg cggagacgat cacccgggtc
    14341 ctcaccgcac tcggcgtgga cgtcacttcc cccgaggcgc tggcgcaggc gcgcgacgag
    14401 gtatggcgaa gcagctggcg acgcacgctg ccgccgacga tcgtctcccg ggagggttgg
    14461 acaccgtggg tgcctgtgca cctgccgcac ggggccgcgg tgcgcgtgga acttgcgctc
    14521 gaggacggcg gcacgatcgc ggtgccgcag gtcgacaact gggtggagcc acggctggtc
    14581 gacggggttc tcaccggcga ggcgacgttc cagttgccgg cggaggtgcc gctcgggtgg
    14641 cacctgctgt gggcgcacgt cgagggccgc gaacccgcat acgcgaccgt cgtcgtcacg
    14701 ccgcagcggc tcgaactgcc ggcgggcctg gaacaccgcc ggatgacggg gctgatgtcg
    14761 caggtctacc agctgcggtc ggccaattcc tatggcgtcg gcgacctggc cgacctcgcc
    14821 gacctggcca cgtggggagc cgccgacctc ggctgcgact gggttttggt caatccgctg
    14881 cacgccgctc aggcctgccc gccgatggag gcctccccct atcttccgac gacccggcgg
    14941 ttcgtcaacc ccctctatat ccgggtcgag gacatccccg aagtggccta cctgtcggtc
    15001 gaggtgcggg ccgccgtcgc cgcgcaggcg cgcaccggcc aggcactgaa tacctcggag
    15061 accatcgacc gcaacgcgtc gtgggcagcc aagcgtgccg cgttggagct ggtgtatgcc
    15121 gcgggcctgt cacctcgtcg cgcccgggcc ttcgagggct tctgcgaacg cgagggtcaa
    15181 ggtctggtcg acttcgccac ctggtgcgcg ctcgccgata tgcacggcga agactggcga
    15241 tcctggcccg caggcctgca gagcccgacg agcccgcagg tcgacgagtt ccgcgccgaa
    15301 caggccgccg agatcgactt cttccgctgg ctgcagtggg tgctcgagga gcaactcgcc
    15361 gcgacccaac ggctggccaa ggaagccggc atggcgctgg gcgtcatcca cgacctggcc
    15421 gtcggtgtcc acggctccgg cgccgactcc tggggcctgg gcgacgcatt ggcccgcggt
    15481 atcgaggtcg gcgccccgcc ggaccagttc aaccagatcg ggcagaactg gagccagccg
    15541 ccctggcgcc cggaccggct cgccgaactc ggctacgcgc cataccgcga catgctgcgc
    15601 acggtgctgc gcgactccgg cggcatccgc gtcgaccaca tcatcgggct gttccggctc
    15661 tggtggatcc cgcagggcat gtcgccgggc gagggcacct acgtccacta cgaccacgag
    15721 gcgctcatcg gcatcctgtg cctggaggca caccgggcgg gcgccgtcgt cgtcggcgaa
    15781 gacctcgggg tcgtccagcc gttcgcccgc gacttcatga aggagcgggg aatcctcggc
    15841 acctcgatcc tgtggttcga gtggcgcgac ggcaaagccc ttgcgccgga aggctatcgg
    15901 gagctctgcc tggcgtccgt cacgacccac gacctggccc cgaccgccgg ctacctcgag
    15961 ctggtgcacg tggcccttcg cgagaagctc ggcctgttga cgcgcgacgc cgcggtcgag
    16021 gaggccgagg agcgggcggc gatcgcccgg gtcgccgagg ccctcgaggc gcgtgggctg
    16081 ccgcacgacc cgagcgatgt cgaggagttc gtcatctcgc tgcaccgcta tctggcgctg
    16141 tcgccggcca agctgctcgg gatcagcgtc cctgacctga tcggtgaccg gcgcgtcgtc
    16201 aaccagcccg gcacggacaa ggagtatccc aactggtgcg tgccgttgac cgggcccagt
    16261 ggtgacctgg tgtcgctgga acagctgacc gcgctgccct acccgcgccg gctcgcccgc
    16321 tccgtcaccg gctgaccccg tccttcgggt ctggcgccgc tcgtcagccg atggcggtgc
    16381 cgaagagccg gccgatggcg taggtgacag ccatcgcgat gatgccgccg gcgacgacgc
    16441 gggctacggc ccggccgatg cgggcgccgc cgagacgcgc gctgacatac ccggtcaggg
    16501 cgagggcgac gaggaccgtg acgaccgtgc cggggaggcg ccacgtgtgc gacgggttga
    16561 gcacggcgat caggggcagc agggccccga gggtgaagga gaccatcgag gcgaacgcgg
    16621 cgtgccacgg attcgtcagc tcgctcgggt cgatgccgag ttcggcctcc gcgtgcgccg
    16681 tcagggcgtc gtggtgtgtc agggccaccg cgacctcgcg cgcgagatcg ggctgaaggc
    16741 ctttcgattc gtagatgccg gcgagttcgt cgagttcgtc ctgcggcttc tcgtgcagtt
    16801 cgcgacgctc cttggcgagc atcgccttct cggtgtcgcg ttgcgtgctg accgagacgt
    16861 actcccccac cgccatgctc atcgccccag ccgcgaggcc ggcgagaccg gcggtgaaga
    16921 ttgcgccccg gttggcggtc gcggcggcga caccgatgac cagaccggcc gtcgagacga
    16981 tgccgtcgtt ggccccgagc acgccggccc gcaaccagtt gagctggctc gccgtgttgg
    17041 cgacgtgctg cgtgggctcg ccctcgtgcg ggctcgccgc cgtggccgtt tcggacggag
    17101 tcgcgttgtc gcgcatgagc tttccagtat gccgtttccg gtgggggcgg tcagggggtg
    17161 tcggtcacct cgaccgtggt ggccggaccg atcaaggatc cgtgcgcgtc cgactccccg
    17221 aggtgggggt cgacgacctc ggcctcgacc ttgtgcggtt gcagttcccc ggacgcgatc
    17281 ccttcggcga aatggcatgc gacacggtgg gtttcgggga cgccgtcgat gctgacgacc
    17341 cgcagcgccg ggcggtcggt gtcacattgc gtgggctgtc gccacgggca ccgggtatgg
    17401 aagcggcatc ccttcggcgg gttcgccggc gagggcagat cgccgttgag caggattcgc
    17461 tcacgccggt cctcgacgac agggtcgggg accggcacgg ccgacatcag cgcacgggtg
    17521 taggggtgca tcggcgcggc atacagatcg tccgcagccg cctcctcgac cagggcaccg
    17581 aggtacatca ccccgacccg gtcgctgata tgccgcacca ccgcaaggtc atgcgccaca
    17641 acgagatagg tcagaccgaa gcggtcttgc aggtcctcga gcaggttgat cacctgggcc
    17701 tgcacggaga cgtccagagc ggagacgggc tcgtccgcga cgatgagttg cggatcgacg
    17761 gccagggcgc gggcgatgcc gatgcgctgg cgctgcccgc cggagaactc gtgcggatac
    17821 ttctgcaagg aggcggcggg caggccgacg gccttgagca gctccttgag ccgggcgttg
    17881 gcggccttgg tgtccttgac gaggccgtgc gccttcatgc cttccagcag aagggattcc
    17941 accgattggc gcgggtcgag gctggagagc ggatcctgga agaccatctg catgtgctgg
    18001 cgtttgcggc gcagcgcctc gcccttgagc gaggcgatgt cggtgccgtc gaagacgacc
    18061 ttgccctcgg tcggcggctc gagattgagg atcgcccgac ccaatgtcga cttgccgcaa
    18121 ccggattcgc cgacgagacc gtaggtctca ccacgccgga tctgcaggtc gacgccgtcg
    18181 acggcataca catagccgat cgtcttgtcg aagatgacgc ccttcttgat ggggaagtgc
    18241 accttgatgc cttgcacgtc aaccagcaca tcgcccggtt gggcgtgcgt ctgctcagct
    18301 gccgccgtca ctggtcgttc acctccgcat ggtcaggttg cgccgtatgc ccgtggggcg
    18361 acccgaccgg gttgtgacac cgcagggcgg acgcgccgtc ctcgacgaac cccggtgtct
    18421 gcgaacggca tacgtcaatg ggctggaaac aacgtggggc gaaagcgcac gcgtgatccc
    18481 acggcaggtt gtcggcgacc gagccgggta tggggatcag cggctccccg cgctggccgt
    18541 cgaggcgggg gatggagccg agtagcccgt gggtgtaggg gtggcggggg gcggcgaaga
    18601 ggtcgtgacg cagtccgcgc tcgacgatgc ggccgccata catgacgttg acctcgtcgc
    18661 acaggccggc gacgacgccg aggtcgtggg tgatcatgat cagcgccgtg ccgctgtcgc
    18721 ggaccaggtc ccccagcagc tgcagaatct gcgcctggat cgtcacgtcg agggcggtcg
    18781 tcggctcgtc ggcgatgagc agacgcggct cgcaggccag tgccatcgcg atcaacgcgc
    18841 gctggcgcat gcctccggag agctggtgcg ggtagtcctt caggcgccgg tcggggtcgg
    18901 ggatgccgac cttgtcgagc atctctcggg ccttgggcat cgccgccttg cggctcatcc
    18961 ccttgtggcg ttcgaggacc tcggtgacct gaacaccgat gggcaccacc gggttcagcg
    19021 agctgagcgg gtcctggaag atcatgccca ggtccttgcc gcgccggtcg cgcatgtctc
    19081 ccgagctcag cttgagcagg tccgtgccgt cgaagtcggc gaccccggtg acgcggttgc
    19141 cgcgggcggg cagcagcccc atgatcgcca gcgaggtcac cgacttgccc gagccggact
    19201 ctcccaccag tccgaccgtc tgccccgggc gcacctcgaa actgacgccg tcgacggcgg
    19261 cgaagggctg ctcgccgtgg cgctggaaga cgacgctgag gtcccggacg gagagcaggg
    19321 cttcgccgct gcgggtgtcc gcggcatacg cgcgtgtggt catggtgctc accgccggct
    19381 cttggggtcg agggcttccc gcagcgactc gcccatgagc gtgaagccga aggcgacgag
    19441 gatgatgcac gccgcgggat agaacgccag gtgcggatag tcgtagatgt acttctgcgc
    19501 cgcgccgagc atctggcccc attcgggctg gcggtcatcg gggttgccga ggccgaggaa
    19561 ggacagggcc gcggcgtcga tgacggcggt cgccaggacc agggtggcct gcacgatcac
    19621 cggcccgagg gagttgggga gcatgtgccg gaagatgatc ggcgcctgct tgacgccgag
    19681 ggcccgggcg gccagcacgt gatcgctatg tcgctgggcg agcatcgaac cacgcagcag
    19741 gcgggcgaag atcggcagat aggcgatcga gatcgcgagg atgacggtcc actggctcgg
    19801 ccgggccgcc atcgccgcca cggagaaggc gagcagcagc gacgggatgg acagcatgat
    19861 gtcgacgaag cgcatgacca cggagtcgat ccacccgccg aaggccccgg cgagcgtgcc
    19921 gaggatcagg ccaccgatga gggagaagag ggtggcgaag acgcagacga gcagggtctg
    19981 gcggctgccg acgatgagcc gcgagagcag gtcgcgaccg acattgtccg cacccagcgg
    20041 gaagcccggc tcggggccgg ggatcggatt gctctgggga cgcaccttgt ccagcagcag
    20101 ccgggcggtc gggtcgtgcg gggccagcaa tggtgcgaag agtgcgacga gcaggaagag
    20161 cacggtgatc gtcaggccga tgaggaagac gcggtcgcgg cgcaggcgcc gccacgcgct
    20221 ggcgatcagg gatacgcctg gcgtgtccgc gacggtgccg gcgtcctgca cctgacccgc
    20281 tgcctgcgcg agggcgtcga tcttggcctt gcggcgggcg ccgggcttcc agcccttggg
    20341 gttgtcggtc atggccgcct cacctcgtcc tcacgcgcgg atcgacgacg gcatacagga
    20401 cgtcgacgat gaggttgacg atgacgaagg tcgctgccga ggccatgatc agcacctgca
    20461 gcacggcgta gtcgcggtgt gagaaggccg acgccaacgc ctcgccgatg ccggggaagg
    20521 agaagacgct ctcggtgagg atggcgccgg cgagcagccc gccgacctgc aggccgacgg
    20581 tcgtgatcac cggaagcaac gcgttgcgca gcacgtgccg gccgcggatg atcctgttgg
    20641 tcagcccctt ggattcggcc gtgcggacga agtcctcgtc cagcacttcg aggacggagg
    20701 cccgggtgat ccggaagatc accgcgaagg ggatcgtcga caaggtgatg gcggggagga
    20761 tcaggtgctt gaaggcgtcc catgcggcgt cccactcgcg cgtcatcagg ccgtcgacca
    20821 cgaagaaatt ggtgacccgg gtggcgtcga tattgctctg gcgacccgag accggcagcc
    20881 agccgagctt gaccgcgaag aagtacttca ggaggaaggc gaggaagaag acggggaccg
    20941 ccacaccgat cagcgagacg atgacgctcg cattgtcgaa cagtgaggcg cgccggcgcg
    21001 cggccaggta gcccaacggg ataccgagga ggatcgcgat gaggatcgcg aagaagctca
    21061 actcgatcgt cgcggggaat cggtcgatga agatctggaa ggcgtcgcgg ccggggacga
    21121 cacccgtcga ggcgccgaag ttgccggtca gggcccgctt gagatagcgc cagtactgca
    21181 catacagcgg ttcgtccagg cccagggcct tggtcagccg ctcgcgtgac tcgggtgtcg
    21241 cgcgatcacc gaggatcgcc gagaccgtgc cgccgggcag catccgcagc cagatgaaca
    21301 acagcagcga cagcacgaag agcacgccga ccatctggag caatcgccgg acgatgaacc
    21361 ggagcaccgc gcctcacctc ccacacgaat cgggacggct gacggccgcc tcggcatcgt
    21421 tgccgaggcg gccatcaccg tctagggcgt gttgccagcc gacgagatca gctcttggag
    21481 accgtgaccg tgtcgaacat ctccgcggtg agcggggatg cggtcaggcc ctcgacgccc
    21541 ttgccgacga cgagcgcggg cgggctgtgg ctgatcggca gaccggggag gtagtcctcc
    21601 atgatcttct ggttgagggc ttcgtatgcc gtggtgcgct cgccctcgtc gacgatcccg
    21661 ttggtctcgt cgagttcctt cgagagggtc ttgccgaagt cggtcgacca cgtgtggaag
    21721 tcgttgctgt cggcgccgaa gaaggtgccg atgaagttgt tggcggagtt gtagtcaccg
    21781 gtccacccga gcagccaggc gtcgaacagg ccgttgtcga cgccgtcgag gtaaccaccg
    21841 ttccacggct tggtgaccgc ctcgaccttg acgccgatgg cctcgaggtc cgcgctgagg
    21901 gcgtcccaga tcttctgggg gtccggcatg tagggccggg agacctcact cgggtaggcg
    21961 aactgcagcg tcataccctc ggcgcccgcc tccttgagca gggccttggc gccctcgacg
    22021 tcgtacttcg tcgcctccag cccggtgttg tatccggcga ccgtgtcggg catgaactgg
    22081 gtggcgacct tggcgccctc gggcaactgg gtcgcgacga gctggtcgcg gttcagcgcc
    22141 atgtagagcg ccttgcgcac ccgcacgtcc tgcagcttgg cgttcttctc cgggttcagg
    22201 cccatgtaga ggatgttgaa cgcggggcgg acgaggacct ggttgccggc gtcctcgagg
    22261 cccttccagt cggccgggtt gggcaggtcg taggcgttga tgctgccggc ctcgagttcc
    22321 tgacggcggg tcgactcgtc ggggatgatc ttgaagacga tgttggcggt cttggccttc
    22381 tcgccccaga agtcgtcatt gcggaccagg gtgatggtct tgttggcctc gtcgtacttc
    22441 tccagcttgt aggggccggt gccgacggga gcgctggcgt aggcggggta cttgaagccc
    22501 tcgccctcct tctggacgtt gttggcgtcg ccgtccttga gcgccttggg gctctgcatc
    22561 gagaacgact cgagggtcag catgaccggg aagtcggagg tgtagcggtt gagggtgatg
    22621 acggcggtcg cgtcgtcctt ggcctcgcag ctcttgtaga gcgactcggt ctcgccgccc
    22681 ttgaagccga gcatcgtgta ggcccagtat tcggccgggc cggcctgcgc gacctcgttc
    22741 tgctcgaaca tccgctcgaa gttgtagcag acggcggcag cgttgaaggc ctcaccgtcg
    22801 gtgaacttca cgccctgacg cagcttgaag gtccaggtca ggccgtcgtc cgagggctcc
    22861 cagctctcgg cgagctcggg ctgggcgtcg gccgtgccgg gcttgatgcc gagcaggccc
    22921 tggaagatct gccgggtgat gcggaaggtc tcgccgtcgg aggcgtagta ggggtcgaac
    22981 acctcggggg caccggcggc gccgaaggtg aacgtgtcgc taccgccctc ggcggccgcc
    23041 gtgccggnnn nnnnnnnggg tcgccgaaac ggtgttcacc ggccgggagt cggtggtgcc
    23101 cgaactggtg gactcgcggt cggactgggc gcaacccgcg agccccaggg agaccgcggc
    23161 ggtcaggacg acggtggtcc tcagtcgcgt gagcttcatt cgctacctca ctaattcatg
    23221 tgcgtccggt cccggggtga ccgaggcgga cctggcgtgg ccttcggcat gccgcgtgcc
    23281 cgggcaggtc tcgcccgcgc ccccggatga ccgaatgctg agcgcacact atgccgcgcg
    23341 gggtatgccc gcgggaaggg cctcgcggtt cgttatgcga tggaaacgtg tcccggcgag
    23401 ggcgaggcac ccgattccgg cgccgagaag caccccggcg aggacatccg tgaggtagtg
    23461 gacgccgagc aggacccgat cggcgcaggt gagcacgacg acggcgccac cgcctgccgc
    23521 cacccaccgg cggcctgacg ccggcatgac cggccacagg acgatgccca gggccagtgc
    23581 cgccgccgtc acgttggagg cgtgcccgga ggggaaggag tagccgccga tcccccacag
    23641 cggatccggc acctgcggcc gtggccggtc gacggcgttc ttcaggatcg gagtgagcca
    23701 ccacgtcagg tatgccgcgg cgagcgccag cagcactcgc cgggtctgac ggtgccgcca
    23761 ccaggccaag gcggcgatga ccgtcagcgc gccgtgggca cagctcggca tgctcgcggc
    23821 ctgccaggcg accagcaggc cgcgcagccc cgcgtcatcg gcaaagcgca ttgccggcgt
    23881 ggagacctca cggtctgcgg cgatcagggc cgcccagggt gcgaggaccg cgaccgtcag
    23941 cagggcgaaa gcgagtaagg cagccagccc agccaccgcc gccgccc
//
LOCUS       DAOPJG010000022        23278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2322_length_23278_cov_7.291780, whole genome shotgun sequence.
ACCESSION   DAOPJG010000022 DAOPJG010000000
VERSION     DAOPJG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 23278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23278
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_23278_cov_7.291780"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKU75_03800"
     CDS             complement(<1..669)
                     /locus_tag="PKU75_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPF80031.1"
                     /translation="MAIFLTENSKVIVQGMTCSEGMKHTARMLASGTNVVGGVNPKKA
                     GTTVDFPEGKQVPVFGGVKEAMDATGADVTVIFVPAAFTKSAVVEAVDAGIPLAVVIT
                     EGVAVRDTAEFFNYSLGKGTRIIGPNCPGLISPGKSNAGIIPADITGPGRIGLVSKSG
                     TLTYQMMFELNDFGFSTGVGIGGDPIIGTTHIDCLQAFQDDPDTDAIVMIGEIGGDAE
                     ERAAA"
     gene            complement(708..1901)
                     /gene="sucC"
                     /locus_tag="PKU75_03805"
     CDS             complement(708..1901)
                     /gene="sucC"
                     /locus_tag="PKU75_03805"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPF80032.1"
                     /translation="MDLYEYQARDLFEKHGVPVLGGAVATTPAEARAAAETIGAKSGG
                     VTVVKAQVKTGGRGKAGGVKVAKSADEAEQYAEQILGMDIKGHTVKKVMIAQGAQIAE
                     EYYFSLLLDRANRTYLALCSKEGGMDIETLAVERPEALARIPVDPNVGIDDAKAAEII
                     QAAGFPEADHAALANVLKQLWEVYHDEDATLVEVNPLVKTEDGQILALDGKVSIDDNS
                     AFRHPEHAEFVDHSKTDPLEVLAIEKGLNYVKLDGNVGIIGNGAGLVMSTLDVVAYAG
                     EEFTGGAAKPANFLDIGGGANAEVMANGLDVIFEDAQVKAVFVNVFGGITACDAVADG
                     IVKAYDILASHGKDPKPLVVRLDGNNADLGRQILDEAKLPLLERVDTMDGAARRVAEL
                     AAAAN"
     gene            complement(2054..3547)
                     /locus_tag="PKU75_03810"
     CDS             complement(2054..3547)
                     /locus_tag="PKU75_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80033.1"
                     /translation="MNERDFVEEFFAREREDITPLEGDDGRWESIVVEARGRRGGRGS
                     RRWVGYAAGLTAAVTLAGIGGWLLRGGLPGSDPNDPLASATSSVSLPTNTQRSSASTS
                     APVVPPPGSSSSSASTQATTGSDATQSSTSGSSSTTNPTNLPVGQDFSLMSVSYAGTD
                     TLLAIGSGTCSGQACPVIIKSTDDGLTWHSAAAIPGADSPGRGAMSYVGNDRAFTGIR
                     MANPSVGWIFGGNIMKSTDGGGHWAAYDHRGAAVVDLATDGKQVVLTSVTGGCDGAKC
                     TGDVLIEGAAIDAAGSTEIKRIPIAVPIDSADVEFTRSGIAVVQVTTVPTAEQPTPLT
                     MTYAVDGGQVSEFGVGCETGYWRYLAAAGGSDDFAACLMPGEGQWTLAKTSGFTGGDP
                     GWAKIEGDQIGLGPGVFNSFAATDAQNLVAASGGSEGQGTIRVSHDGGRTWREPKQAP
                     PLPDRGWRWVASPGADWFYAVPADGFRGFWRSTDNGETWTRVTLGPR"
     gene            complement(3544..4044)
                     /locus_tag="PKU75_03815"
     CDS             complement(3544..4044)
                     /locus_tag="PKU75_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPF80034.1"
                     /translation="MPYGADVISADEAAVREVYRAHYGMLAGWCNKLVGDRDLAHDVA
                     TEAFVRLLAHWGDVAEPRAWLYTAAANIIRDHWRKRGREQVAYGKVGHDIETGAEPDL
                     ARDLSVRAAVQALPDRLRVSVMLHYFADLSIAQVATQLGKSEGAVKRDLWDARQRLAA
                     SLEGAR"
     gene            complement(4217..4423)
                     /locus_tag="PKU75_03820"
     CDS             complement(4217..4423)
                     /locus_tag="PKU75_03820"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HPF80035.1"
                     /translation="MKAQVISGTTTTTLATYSNLSATGGAFVQKSFDLTAYKGKTVTV
                     KFLSQEDSSLQTSFVVDDVAVTTS"
     gene            4407..4589
                     /locus_tag="PKU75_03825"
     CDS             4407..4589
                     /locus_tag="PKU75_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80036.1"
                     /translation="MTCAFIVSYAVLLVVSAVSIRTQKVKVAVVAEAGIVTDWNAFSL
                     TVRPLPPSHSFQLSNS"
     gene            4778..5587
                     /locus_tag="PKU75_03830"
     CDS             4778..5587
                     /locus_tag="PKU75_03830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPF80037.1"
                     /translation="MSRYTGRHAAARPGTLRRLGDPRIAAPIAAAGLVLAATSGSLGA
                     DAASAAAAPGTQASVSSAAVQALADRSGTREAAVAAAASIDTAGELAADATREQSTVR
                     AQRTAERQALVVKKAKEAAKKKAEAKKKAAAAARIKAAHAWVSPIKNPRLTSGFGARW
                     GRLHAGLDFGAVVGTPLRSLSTGTVTEAGWGGGYGQKVEITYWDGTVSYFAHMSVISV
                     TKGQKVTPGMIVGKSGNTGHSTGPHLHLEIHPGGGGPVDPRGWLAKHGLKL"
     gene            complement(5749..8172)
                     /gene="pcrA"
                     /locus_tag="PKU75_03835"
     CDS             complement(5749..8172)
                     /gene="pcrA"
                     /locus_tag="PKU75_03835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HPF80038.1"
                     /translation="MTTLFDESPEWAGAVTDAGIPTWAMTQEGPAKRHRDTEAGEREA
                     SGRHTDPEALLGGLNTQQREAVIHEGGPLLIVAGAGSGKTRVLAHRIAYLLGHRGVLP
                     GQILAITFTNKAAGEMRERVAGLVGPRAKAMWVMTFHSACVRILRREASKVGLKSNFS
                     IYDATDSQRLMSLVLRDMDLDPKRYNPRAFSHQVSNLKNELVDEETYAARVGSEETGT
                     HQERLLAEAYRTYQRRLRQANALDFDDLIMTTVNILQAFPDVAEHYRRRFRHILVDEY
                     QDTNHAQYVLVRELVGGPGTSGYAADTPSVVPPGELVVVGDADQSIYAFRGATIRNIE
                     EFEKDYPDARVILLEQNYRSTQTILKAANAVIRTNSGRREKNLWSEAGDGDPIIGYVG
                     DSEHDEAAFVARRIDTLGDDHEVKPKDVAVFYRTNAQSRAIEEVFVRVGLPYKVVGGT
                     RFYERKEIKDALAYLRVLANPEDTINLRRILNVPKRGIGDRAEACVAALAERERIPFV
                     AALGRAADAPGIATRSVAAIEGFTSLLESLRQVRDDDGAGVADLLEAVLDRSGYVAEL
                     KASRDPQDETRLENLHELLSVAREFDEVRIETGEVISLDDFLEQVSLVADADEIPDAE
                     ADGGVVTLMTLHTAKGLEFPVVFLTGLEDGTFPHQRSLGGDPAELEEERRLAYVGITR
                     ARERLHISRASVRSAWGAPQYNPPSRFLDEIPDGLITWERTDSSPGQRTAAPSLARLA
                     ARPGVRSPGNRPMLALKVGDRVSHDTFGLGTVTRVDGADERTQVHVDFGGEAGVKRLL
                     LRFAPLEKL"
     assembly_gap    8225..8324
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8325..9356
                     /locus_tag="PKU75_03840"
     CDS             <8325..9356
                     /locus_tag="PKU75_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80039.1"
                     /translation="ANGRYVVFASDASNLVAGDTNGKRDIFIRDLQSQTTRLVSKGRS
                     GPSNGMSTTPKVSDDGRYVSFLSTASNLVARDSNGKADVFRVDTTTGTIIRVSVGAGG
                     VQANGASEGPRISANGNSIVFGSAASNLVRGDTNKVNDIFLRDVSTGQTRRLSMTPQG
                     NQNLAASDMSSISPNGSIASWRGPVGLHHAVFSWDRASNTVRNVAESNSGDYRAIRTS
                     NAGPSWLLESYVYPSMYWEYKITAAPKPASFRWFAQDPEQSHLVDFDVPMWGAMGVFA
                     RSDSYDPDNHGVVVADTTGELAVLRPTPWNALGFSSDGNHVVGADESTGQIKRWSWRT
                     GVVTIVSVN"
     gene            9372..>9559
                     /locus_tag="PKU75_03845"
     CDS             9372..>9559
                     /locus_tag="PKU75_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80040.1"
                     /translation="MIRRIGTITITLAAAAACAAAATGTAGAAAATASGNKGSYGPDV
                     SATGRYVVFASDATNLVAG"
     assembly_gap    9560..9659
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9660..10311
                     /locus_tag="PKU75_03850"
     CDS             <9660..10311
                     /locus_tag="PKU75_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80041.1"
                     /translation="NHATDIFLRDLPGTIRRVSLMPTGDQLSNAASQATISNDGARVG
                     FMGLSGSSDAIFQWSRRLQRTTILSEEASDHQVGSPVTSNGGVSSAGEETNFDCWNSG
                     ISVTASPKSESFYIDDDCGSTVDYGYDVSRWGAMASIAYFDYSINATLLYVVDDYFEL
                     SYPDRYPPLARKRVNASAVSMAADGSRVGYATTIGGISQIQLWNWKTGATATVSTS"
     gene            10390..11220
                     /locus_tag="PKU75_03855"
     CDS             10390..11220
                     /locus_tag="PKU75_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HPF80042.1"
                     /translation="MTFSIVAREGRAFGVAVASKFLAVGGVVPAARLGTGAIATQSYA
                     RVSFKADLLSHLARGNSASRALAAVTADDLEAMKRQVGVVGSKDAATFTGSGCHPWAG
                     GTSGEGSNGDDSAYAIQGNVLTGPEVIAEMERAFLDSAGQSFTRRLLASLMAGDAAGG
                     DSRGRQSAALYAVAPKAGYDRSGVLADLRVDDNPDAINELARIHDLHELYFGAPEDVQ
                     PLIGDLELEVRSRLDGLGYRGRPLADDLALWAGEANLETRLTRDGIDGRVLEELRKAG
                     "
     gene            complement(11445..11627)
                     /locus_tag="PKU75_03860"
     CDS             complement(11445..11627)
                     /locus_tag="PKU75_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80043.1"
                     /translation="MHLERRQWRKDLRRREALEADGWRVIVVIAADLHDVPALNRRVA
                     EALLARGMRWPAVSAG"
     gene            12135..12683
                     /locus_tag="PKU75_03865"
     CDS             12135..12683
                     /locus_tag="PKU75_03865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80044.1"
                     /translation="MRLIPPTTRLHRSYLGGVDEFAGARRDGDGLWQWVEDGVTHEIN
                     REEMESEDGFARFVARRTDPDRDLLPGWVPCTFFWMEQDGEYAGSLALRHELNEFLAA
                     EGGHIGYSVRPSARRRGHATEALRQSLPHARALGLDRVLVTCDETNIASARTIEANGG
                     VLAKTVEAASDGRLVRHYWIAL"
     gene            12694..13176
                     /locus_tag="PKU75_03870"
     CDS             12694..13176
                     /locus_tag="PKU75_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80045.1"
                     /translation="MPLIREARPADVPRILALVRDLAHYEKEPDAVEATEADFTHALF
                     PTEGAPTAYGHVAEVDGEMVAMAIWFLTFSTWTGQAGIHLEDLYVAPAQRGSGLGTAL
                     LTRLAQVCVERGYRRLEWAVLTWNTPAIEFYDALGAEPMTAWTDYRLDGAALQRVGAH
                     "
     gene            13217..14194
                     /locus_tag="PKU75_03875"
     CDS             13217..14194
                     /locus_tag="PKU75_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HPF80046.1"
                     /translation="MTTTRALTVTANGGSAVLAVQHVDVPDPGPGQAQVKVAAAGVNF
                     IDVYQRQGVYPIPTPFTLGMEGAGEVVAVGEGVDDVAVGDRVAWAMHQGTSAGLANIA
                     AAQLIPVPDGLDFKVAAAAALQGMTAHYLVNSTYAVQPGDVALVHAAAGGVGQLLVQM
                     ITAKGGTVIATAGGPEKLAIAERLGASTLIDYRSEDVAAAARAATDGTGVHVAYDGVG
                     KDTFDASLASLRPRGMVVLFGGASGQVPPFDLQRLNAAGSAYVTRPSLAHYLLTRDEL
                     LWRGREVFAALADGSLELEVGGEWPLEEAAAAYDALEGRQSTGKLLLTP"
     gene            complement(14226..14591)
                     /locus_tag="PKU75_03880"
     CDS             complement(14226..14591)
                     /locus_tag="PKU75_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80047.1"
                     /translation="MEILINVLVLVHLIGMACIVGGWIALRARGVESGRGLSVVVWGA
                     RFQILTGLALVGIHESQNDPLNHAKVGVKLTVALACAAAAEMAAAKARKGSGDSNLID
                     YAGVLGILNTAIAALWETS"
     gene            complement(14601..15821)
                     /locus_tag="PKU75_03885"
     CDS             complement(14601..15821)
                     /locus_tag="PKU75_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HPF80048.1"
                     /translation="MLSSYHLRPASGWINDPNGLTFHDGRWHVFYQHNPAGPVHGDIH
                     WGHASSADLVVWEHHPVAFGPQTGGPDAGGCWSGVFVPGLDRPAVVYSGVVASAADAT
                     VCLRWGSPDLLSWGAPQVVAEPPPGVREMRDPFVFDWEARRFALVGAGLHDGTAALLL
                     FDASEITAWRYVGVVARADAPVCRTLPPSDVWECPQLCSVDGVTVLILSLCTHGAPPG
                     PVVWVAVDIDAASYGADRRVRVEAGPQLAAIAWGLVDTGPNFYAPQVMTDDEEPMLIG
                     WIPSRTPHDPDVPVAGCLTLPRRLAWQDGALVSRIDSVAAQALTGSGAGQEVGSGRHT
                     LPGAARADVPAGRLTSSSDPALSLQLTDAEVWVDADVVEVYPAGGPAWTARGTGEWLL
                     EIPEDLTGTLQLLV"
     gene            complement(15832..16545)
                     /locus_tag="PKU75_03890"
     CDS             complement(15832..16545)
                     /locus_tag="PKU75_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF80049.1"
                     /translation="MTATTTPKPATPKPPVRVVIVDDHKMFRTGVRAELGEVAGTALE
                     IVGEAPDVDEAIKVIHATHPDVVLLDVHLPGGNGRGGVDVLAGCVAQDSLHGQPVRFL
                     ALSVSDAADDVIAVIRAGARGYVTKTITGTDLATAIRRVADGDAVFSPRLAGFVLDAF
                     GASAGEIAEVDEELDRLSQREREVMRLIARGYQYKEVARELFISVKTVETHVSSVLRK
                     LQLSSRHELTTWAMGRKLI"
     gene            complement(16542..17795)
                     /locus_tag="PKU75_03895"
     CDS             complement(16542..17795)
                     /locus_tag="PKU75_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPF80050.1"
                     /translation="MPMPYAPATAAPAATRPRMVRPSGERWIAGVCAGLAEHLHLPVV
                     WVRIGMAALGFSGPGLVAYVLLWALTPQEDTRVQTVDPAAASGVADPALSPASRPMRR
                     GAVLFGLGLMALGMMTGITRAAGPRLSLIVPLAAIGAIAVLGLTSADAAQRDRWLGRR
                     SGWPALARLGAGALAVVVGAIVVLSRGQSVGAASDIAVATIVVLAGMLLLLAPLGLRL
                     LSDSAEARSAQIRADERADIAAHLHDSVLQTLALIQRQSGDPARVATLARVQERELRA
                     YLYGADGDPETSLSGAIARVVDEIEVAHGIPIDVVVTGDREVDDHVLALVRALREALN
                     NAARHGAPPIAVYVEAGPGEVEAFVRDHGPGLDLDAIEEDRLGVRESIIGRMTRAGGT
                     AKLRRLDPGTEWTLTLPTPETGGPR"
     gene            17920..>18738
                     /locus_tag="PKU75_03900"
     CDS             17920..>18738
                     /locus_tag="PKU75_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPF80051.1"
                     /translation="MTTPPPPAPAPGPSSSSAPGREPIVDEFTSAVARLGIRRTTEGW
                     VGGVCDGIALRWSIDPVVVRVACIVLALFGGFGIAVYAAGWLLLPAPDGPSLLSRISA
                     SAGHTVALLALAGLALVVIVFSGSGLYDPQGWGLSQLLLLLVIGGVGYAALRSRPGPG
                     TAPPPPAQQAPPGAAGSPGVAGSSGATGSSGATGLTGAAGSSVAAGSSGGAGSPGGAP
                     ETHPTPLWPPVHRVPATPGGAGASDVEHTLPDPVWPDPVWPDHGSPGPGSPGPGS"
     assembly_gap    18739..18838
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19268..20002)
                     /locus_tag="PKU75_03905"
     CDS             complement(19268..20002)
                     /locus_tag="PKU75_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF80052.1"
                     /translation="MSRGIRIVGVTLAGDEVVDVVLPHGADPSDELHRRGWSMTGVRQ
                     AFTPSDEEGVTIQVSVVAEVGEERHTTWVPATSGERPPEGVDAVVVQRLAAYAVVVRD
                     GLVLATRLAADVRGAAHRWTLPGGGVDPGEDPATGLRREVWEETGQYLGEIRLLDLLT
                     SHWVGQAPDGTWEDYQVVRLVYAATVPEPGPLVVHDQGGTTSEAAWLDVDALAPHQRA
                     PLLAADRWLTWRAAAGDQGLRGPRQP"
     gene            complement(19999..21141)
                     /locus_tag="PKU75_03910"
     CDS             complement(19999..21141)
                     /locus_tag="PKU75_03910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF80053.1"
                     /translation="MKIALLSDCYPPRLGGIETQVRDLGRQLAAAGHEVHVYTATPES
                     RPRLRLRNVTVAADSGVTVHRVTLPLPFALPVNPVAPSIIRDELRTFDVAHVHMGVVS
                     PFAADMANLALDLAVPTAITWHCVLGEAAIAAHRRLGTAARWRARGAALSGVSRMAGR
                     LVGRAAGVDDGVGGDAIAVLPNGIDAASWRLDGMPPARDSGRVRVVAAMRFAPRKRVL
                     ALLAMLDEVHDADPDRVEATVYGDGPLLAPARLRPSSRDRGWLHLPGRVTRPALAAAY
                     AAADVYLAPTRMEAFGIAALEARTAGLPVVAIGSSGVADFITHDVNGLLAEDDAGLVA
                     ATRRLVAHDALRQRIRAHNLATPPAEDWSHIVECVLAEYRRAGATR"
     gene            21210..22049
                     /locus_tag="PKU75_03915"
     CDS             21210..22049
                     /locus_tag="PKU75_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPF80054.1"
                     /translation="MTTSRPSGGRRTLVAFHAHPDDEALLTAGTMAKAAAAGHRVVLV
                     LATNGDLGLTDSATRARDLGAQRLAEARRSADELGVARVEWLGYADSGMGPELYADPP
                     GRTRFVRAPVAEAAQRLAVLLREESADVLLSYDARGGYGHRDHVRVHEVAAAAARLAG
                     TPRVLEATIPRDLLAAAVRLSARVYRYPPEFDIEGFCSAFSARRDITHRIDVLRFAAA
                     KRAAMRAHASQALADGADRTLAAFLRIPRPAYDLIFGREWFRNPAHPAGAGVARDIFA
                     GLP"
     gene            22046..23134
                     /locus_tag="PKU75_03920"
     CDS             22046..23134
                     /locus_tag="PKU75_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPF80055.1"
                     /translation="MSTARETARDSAARPHFGRTQLLQAAAGLALSAALLIWGLPRFA
                     QTSWHEVWAVLTTITVGNALIFLGLVLLGLWSYTLTLSASMPGLSHPRALVMNVCGSS
                     VSNLLPGGGAVGLAATYAIARSWGISRRVVSTSAIVTGVWNTLARIALPLIAIAALTI
                     GRVGMTPVLRDAAIAAMISGTVVVVLFVGALASERVARRVGRGLDRLLAPLTRRTKRT
                     MTIDALVTDLRSRILHIVAKGWLPMTLGLVGYFGFYYLTFVAIMRNTGVELFHGELFA
                     AFAIGRLLTAVGITPGGMGVTETGTAAALVAWGAAPAEAAAGVVLFSIFTHLMEVPLG
                     ALGWLAWSVIPKATEPDLTDPDPPPDLP"
BASE COUNT         3377 a         8119 c         8017 g         3465 t
ORIGIN      
        1 ggccgcggcc cgctcctcgg cgtcgccgcc gatctcgccg atcatgacga tcgcgtcggt
       61 gtcggggtcg tcctggaagg cctggaggca gtcgatgtgg gtggtgccga tgatcgggtc
      121 accgccgatg ccgacgccgg tggagaagcc gaagtcgttg agctcgaaca tcatctggta
      181 ggtcagcgtg ccggacttgc tgacgaggcc gatgcggccg gggccggtga tgtcggcggg
      241 gatgatgccg gcgttggact tgccggggct gatcaggccg gggcagttgg ggccgatgat
      301 gcgggtgccc ttgccgaggc tgtagttgaa gaactcggcg gtgtccctga ccgccacgcc
      361 ctcggtaatg acgaccgcga gcggtatgcc ggcgtcgacc gcctcgacga ccgccgactt
      421 ggtgaaggcg gccgggacga agatgacggt cacgtcggcg cccgtcgcgt ccatcgcctc
      481 cttgacgccg ccgaagacgg ggacctgctt gccctcgggg aagtcgaccg tggtgccggc
      541 cttcttgggg ttgacgccgc cgacgacgtt ggtgcccgag gccagcatcc gggcggtgtg
      601 cttcataccc tcggagcaag tcatgccctg aacgatgacc ttggagttct cggtgaggaa
      661 gattgccatg gctgctgtat gccgtcctgt tccgtagtct cttgcgttca gttcgcggcc
      721 gcagccagtt cggcgacccg gcgggctgca ccgtccatcg tgtccacccg ctcgagcagg
      781 ggcagcttcg cctcgtcgag gatctgccgg ccgaggtcgg cgttgttgcc gtcgagccgg
      841 accacgagcg gcttggggtc cttgccgtgg ctcgccagga tgtcgtaggc cttcacgatg
      901 ccgtcggcga ccgcgtcgca ggcggtgatg ccgccgaaga cgttgacgaa gacggccttg
      961 acctgggcgt cctcgaagat gacgtcgagg ccgttggcca tcacctcggc gttggcgccg
     1021 ccgccgatgt cgaggaagtt ggcgggcttg gccgcgcccc cggtgaactc ctcaccggcg
     1081 taggcgacga cgtcgagggt ggacatgacc agtccggcac cgttgccgat gatgccgacg
     1141 ttgccgtcga gcttgacgta gttgaggccc ttctcgatgg caagcacctc gagtgggtcg
     1201 gtcttggagt ggtcgacgaa ctcggcgtgc tccgggtggc ggaacgcgga gttgtcgtcg
     1261 atggagacct tgccgtcgag ggcgaggatc tgaccgtcct cggtcttgac gagcgggttg
     1321 acctcgacga gcgtggcgtc ttcgtcgtgg tagacctccc acagctgctt gagcacattg
     1381 gcgagcgcgg cgtggtcggc ctcggggaag cccgccgcct ggatgatctc ggccgccttg
     1441 gcgtcgtcga tgccgacatt ggggtcaacg gggatgcggg ccagcgcctc gggacgctcg
     1501 acggcgagcg tctcgatgtc catgccgccc tccttggagc acagggcgag gtaggtgcgg
     1561 ttggcgcggt cgagcagcag ggagaagtag tattcctcgg cgatctgcgc gccctgggcg
     1621 atcatgacct tcttgaccgt gtggcccttg atgtccatgc cgagaatctg ctcggcatac
     1681 tgctcggcct cgtcggcgga cttggcgacc ttgacgccgc cggccttgcc gcggccgccg
     1741 gtcttgacct gcgccttgac gacggtcacc ccgccgctct tggcgccgat ggtctcagcc
     1801 gccgcgcgcg cctcggccgg ggtcgtcgcg accgcgccgc ccaggacggg tacgccgtgc
     1861 ttctcgaaaa ggtcgcgagc ttggtactcg taaagatcca cgctgatggc cgtccttcgc
     1921 tccggtgcgc tgttcgggga tgccttacga gcctagaggg tcagcctcga tgcttccgac
     1981 cacaccacgc atcgagcacg tcacaaccgc ccccgctcgg cgcacagtcc gaccgctcac
     2041 ccccacccgg cccctagcgc ggcccgaggg tgacgcgggt ccaggtctcg ccattgtcgg
     2101 tggagcgcca gaagccgcgg aagccgtcgg cggggacggc atagaaccag tcggcgcccg
     2161 gcgaggccac ccagcgccag ccccggtcgg gcagtggcgg ggcttgtttg ggctcgcgcc
     2221 aggtccggcc gccgtcgtgc gagacgcgga tggtgccctg cccctcgctg ccgccggagg
     2281 ccgccacgag gttctgcgca tcggtggcgg cgaaggagtt gaagacaccg ggcccgaggc
     2341 cgatctggtc gccctcgatc ttggcccagc cggggtcgcc gccggtgaag ccgctggtct
     2401 tggcgagtgt ccactggccc tcacccggca tcaggcatgc ggcgaagtcg tcgctgcccc
     2461 cagccgcggc caggtagcgc cagtagccgg tctcgcaccc gaccccgaac tcgctcacct
     2521 ggccgccgtc gacggcatag gtcatggtca ggggcgtggg ctgctcggcc gtcggcacgg
     2581 tcgtgacctg gacgacggca atgccgctgc gggtgaactc gacatcggcg gagtcgatcg
     2641 gcaccgcgat cgggatgcgc ttgatctctg tgctccccgc ggcgtcgatg gccgcgcctt
     2701 cgatgagcac gtcgccggtg catttggccc cgtcgcaccc cccggtcacc gacgtgagca
     2761 cgacctgctt gccgtcggtg gcgaggtcca cgaccgccgc gccccggtgg tcgtatgccg
     2821 cccagtggcc gccgccgtcg gtgctcttca tgatgttgcc accgaagatc cacccgacgg
     2881 aggggttggc catccggatg ccggtgaacg cgcggtcgtt gccgacatag ctcatcgccc
     2941 cgcggcccgg gctgtccgcc ccggggatgg cggcggcgga atgccacgtc agcccgtcgt
     3001 cggtcgactt gatgatgacc gggcacgcct gccccgagca ggtgccggac ccgatggcca
     3061 gcaacgtgtc cgtgccggcg tagctgacgg acatcagcga gaagtcttgg cccacaggca
     3121 gattcgtcgg gttggtcgta gacgagctac ccgaggtcga cgactgagtg gcgtcggacc
     3181 cggtggtcgc ctgcgtgctt gcactgctgg aggaggaccc gggcggcgga accacgggcg
     3241 cactcgtcga ggcgctcgag cgctgcgtat tggtgggcag gctcaccgaa ctggtcgcgc
     3301 tggccagcgg gtcgttggga tccgatccgg gcaggccacc gcgcagcagc cagccaccta
     3361 tccccgcgag cgtgaccgcg gcggtcaggc ccgcggcata cccgacccag cggcgcgagc
     3421 cgcgaccgcc ccggcgtccc cgcgcctcga cgacgatcga ctcccagcgt ccgtcatcgc
     3481 cctcgagcgg ggtgatgtcc tcgcgttctc gggcgaagaa ctcttcgacg aagtcgcgct
     3541 cgttcatcgg gcaccttcca aactggcggc caggcgttgc cgggcatccc acagatcacg
     3601 tttgacggcc ccctcggact tgccgagctg cgtcgccacc tgggcgatcg acaggtcggc
     3661 gaagtaatgc agcatcacgc tgacgcgcaa ccggtccggc agcgcctgca cagcggcccg
     3721 caccgacaga tcgcgggcga gatccggctc cgccccggtc tcgatgtcgt gcccgacctt
     3781 gccgtaggcc acctgctcgc ggcccctctt gcgccagtgg tcgcggatga tgttggcggc
     3841 ggcggtgtag agccacgcgc gcggttcggc gacgtccccc cagtgcgcca ggagccggac
     3901 gaaggcctcg gtcgcgacgt cgtgagcaag atcccggtcg ccgacgagct tgttgcacca
     3961 gccggcgagc atgccgtagt gggcgcggta gacctcccgg accgctgcct cgtcggcgct
     4021 gatgacgtca gctccgtagg gcatgctcac cccccagggg cggacgcgag gcagcgcgat
     4081 cgttgtcatg cctcattgac gccgcactct ccccctgggt tggtccccaa tttttctcgg
     4141 tgcatcgtac ttgtcgcgca cgcgcgaggc agggaggccc ggttggcacc tccccgcctc
     4201 gggtatgcgg cgaacgtcag ctcgtcgtga ccgccacgtc gtcgacgacg aaggaggtct
     4261 gcagcgaaga atcctcctgc gagaggaact tcaccgtgac cgtcttgccc ttgtatgccg
     4321 tcaggtcgaa cgacttctgg acgaaggccc caccggtcgc cgacaggttg gagtaggtcg
     4381 ccagggtcgt cgttgtggtg ccggagatga cctgcgcctt catcgtgtcg tatgccgtgc
     4441 tgctcgtcgt ctcggcggtg tcgatccgca ctcagaaggt gaaggtcgcc gtggtcgccg
     4501 aggcggggat cgtcaccgac tggaacgcgt tctcgctgac ggtgcggccg ttgccgccga
     4561 gccacagctt ccagctgagt aactcttaac ctcccgcgcc ttgacaaggt caaaggtgct
     4621 ggtcatcagg cgtcttggcg cccgcatacc gcagctacgg cgtcggacgt gtccagtgac
     4681 acatgcttga attttggata acgaaacgat cacgctaccg tggtgggagc gtccccctcc
     4741 cccgggctcc ccccagcccc catccgaagg accatctgtg agccgttaca ccggccggca
     4801 cgctgcggcg cgcccgggca ctctgcgccg cctcggcgac ccgcggattg ccgctcccat
     4861 cgccgccgcg ggcctcgtcc tcgccgccac ctccgggtct ctcggtgccg acgcggccag
     4921 cgccgccgcc gcccccggca cccaggccag cgtgtccagt gccgcggtcc aggccctcgc
     4981 ggatcgctcc ggcacccggg aggccgccgt tgctgcggcc gcgtccatcg acaccgccgg
     5041 cgaactggcc gcggacgcaa cgcgtgagca gagcaccgtc cgcgcgcagc ggaccgccga
     5101 gcgccaggca ctggtcgtga agaaggccaa ggaggcggcc aagaagaagg ccgaggcgaa
     5161 gaagaaggcc gcggctgccg cccggatcaa ggccgcgcac gcctgggtct cccccatcaa
     5221 gaacccgcgc ctgacctccg gcttcggcgc ccgctggggc cgcctgcacg ccgggctcga
     5281 cttcggtgcc gtagtcggca cccccctgcg gtccctctcg accggcaccg tgaccgaggc
     5341 cggctggggc ggcggctacg gtcagaaggt cgagatcacc tactgggacg gcaccgtttc
     5401 ctacttcgcc cacatgagcg tcatctccgt gaccaagggc cagaaggtca cgcccgggat
     5461 gatcgtcggc aagtctggca acaccggcca ctccaccggc ccgcacctgc accttgagat
     5521 ccaccccggc ggcggcggcc ccgtcgaccc ccgcggttgg ctcgccaagc atggcctgaa
     5581 gctctgacgc tccggccagt cccggccccc cccaagtaac ctctgctagc agactttact
     5641 caaatcagca cactttccta ggtgtgctga tttgactagc ctctgctagc agaggctagg
     5701 tggagagctc ccgacggccg tggcgcgcaa caggtttggc gcggtgggtc agagcttctc
     5761 gaggggggcg aaccggagga ggaggcgctt gacgccggcc tcgccgccga agtcgacgtg
     5821 cacctgggtg cgctcgtcgg cgccgtcgac gcgggtgacg gtgccgagac cgaacgtgtc
     5881 gtggctgacg cggtcgccga ccttgagcgc cagcatcgga cgattgccgg gtgagcggac
     5941 cccggggcgg gccgccaggc gcgcgaggga cggggccgcg gtgcgctggc cggggctgga
     6001 gtcggtgcgc tcccacgtga tcagcccgtc ggggatctcg tcgaggaacc gcgagggcgg
     6061 gttgtattgc ggtgcgcccc aggcagatcg cacgctcgcc cggctgatgt gcaggcgctc
     6121 gcgggcccgg gtgatgccga cataggccag gcgccgctcc tcctccagct cggccgggtc
     6181 accgccgagg gagcgctgat gggggaaggt gccgtcctcc aacccggtga ggaagacgac
     6241 ggggaactcc aaccccttgg ccgtatgcag tgtcatcagg gtgacgacgc cgccgtcggc
     6301 ttcggcgtcg gggatctcgt cggcgtcggc gacgagggag acctgctcga ggaagtcgtc
     6361 gagggagatg acctctccgg tctcgatccg aacctcgtcg aattcccttg caacggacaa
     6421 gagttcgtgg agattctcga gacgggtctc gtcctgcggg tcacggctcg ccttcagctc
     6481 cgccacgtag ccggacctgt cgagcaccgc ctccaacagg tcggcgacac ctgcgccgtc
     6541 gtcgtcgcgc acctgccgca gcgattccaa caaggatgtg aagccctcga tcgccgccac
     6601 cgaccgggta gcaatgccgg gcgcgtcggc cgcccggccc agggcggcga cgaaggggat
     6661 ccgctcccgc tcggcgagcg cggccacgca cgcctcggcc cggtcaccga tgccgcgctt
     6721 ggggacgttg aggatgcgcc gcaggttgat cgtgtcctcg ggattggcga gcacccgcag
     6781 ataggcgagc gcatccttga tctccttgcg ctcgtagaag cgcgtgccgc cgacgacctt
     6841 gtagggcagg ccgacgcgga cgaacacctc ttcgatggcg cgcgactggg cattggtgcg
     6901 atagaagacc gcgacgtcct tgggtttgac ctcgtggtcg tcgccgaggg tgtcgatgcg
     6961 ccgggcgacg aaggccgcct cgtcgtgctc ggagtcgccg acataaccga tgatcggatc
     7021 gccgtcgccg gcctcggacc agaggttctt ctcgcgccgt cccgagttgg tgcggatgac
     7081 cgcattggcg gccttgagga tggtctgcgt ggagcgataa ttctgttcca gcagaatgac
     7141 tctcgcgtcg ggatagtcct tctcgaactc ttcgatattg cggatcgtcg ccccgcggaa
     7201 ggcatagatc gactggtcgg cgtccccgac gaccacgagc tcacccggcg gcacaacaga
     7261 tggtgtgtcc gcggcatacc ctgacgtgcc gggaccaccc accaactcgc gcaccaggac
     7321 gtactgggcg tgattggtgt cctggtactc gtccaccagg atgtgccgaa agcggcgccg
     7381 atagtgttct gcgacatcgg gaaaggcctg caggatgttg accgtcgtca tgatcaggtc
     7441 atcgaagtcg agtgcgttgg cctgccgcag ccgccgctga taggtgcgat acgcctccgc
     7501 gagcaaccgc tcctggtgag tgcccgtctc ctcgctcccc acccgggcgg cgtaggtctc
     7561 ctcgtcgacc agctcgttct tgaggttgga gacctggtgg gagaacgcgc gcgggttata
     7621 gcgcttggga tcgaggtcca tgtcgcgcag cacgagcgac atcagccgct gactgtcggt
     7681 ggcgtcgtag atggagaagt tggacttcag cccgaccttg ctggcctcac gacgcaggat
     7741 ccgcacgcac gcggagtgaa aagtcatgac ccacatggct tttgcccgcg gcccgacgag
     7801 cccggcgacc cgctcgcgca tctcgccggc ggccttgttg gtgaaggtga tcgcgaggat
     7861 ctgccccggc agcacgccgc ggtggccgag caggtaggcg atgcggtggg cgagcacgcg
     7921 ggtcttgccg gacccggcac cggcgacgat caggagtggt ccgccctcgt ggatcaccgc
     7981 ctcgcgctgc tgggtgttga gtccgccgag cagggcctcg gggtcggtat gccgcccgct
     8041 cgcctcccgc tcccccgcct cggtgtcgcg gtggcgcttg gccggtccct cctgggtcat
     8101 cgcccacgtg ggtatgccgg cgtcggtcac cgcgccggcc cactcggggg attcgtcgaa
     8161 caaagttgtc atcgcgtgct ccagcctacg tggcaccgcc gacaggtctt gcccactacg
     8221 cgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccaat ggacgctatg
     8341 tcgtattcgc gtcggacgcc agcaacctgg tggccggtga cacaaacggc aagcgcgaca
     8401 tcttcatccg cgacctgcag tcgcagacta cacgcctcgt ctcgaagggc cggagcgggc
     8461 cgtcgaacgg catgagcacc acaccgaagg tgtccgacga cggacgttat gtctcgttcc
     8521 tgtcaacggc aagcaatctc gtggcacgcg acagcaacgg caaagccgac gtcttccggg
     8581 tcgacacgac gacgggcacc atcatccgcg tcagcgtggg ggcaggagga gtgcaggcca
     8641 atggcgcatc ggagggtccg cggatctcgg ccaacggcaa ttcgatcgtc ttcggctccg
     8701 ccgcatccaa cctcgtcagg ggcgacacca acaaggtcaa tgacatcttc ctgcgcgacg
     8761 tgtccaccgg tcagacccgt cggctgtcga tgaccccgca gggcaatcag aacctcgccg
     8821 cctccgacat gagttcgatc agccccaacg ggtccatcgc ctcatggcgc ggtcccgtgg
     8881 gcctgcacca cgccgtcttc agctgggacc gggcgagcaa caccgtgcgg aatgtcgcgg
     8941 agtccaactc cggggactat cgggcgatcc gcaccagcaa cgcggggccg agctggctgc
     9001 tcgagtccta cgtctaccca tcgatgtatt gggaatacaa gatcaccgct gcaccgaaac
     9061 ctgcgagctt caggtggttc gcgcaggacc cggagcaatc ccatctggtc gatttcgacg
     9121 tgccgatgtg gggcgccatg ggcgtcttcg cccgctccga ctcctacgat cccgacaacc
     9181 acggggtcgt cgtcgccgac acaaccggcg agttggccgt gctgcgtcca accccgtgga
     9241 acgcgctggg cttctcgagc gatggaaacc acgtcgtcgg agctgacgag tcgaccggac
     9301 agatcaagcg ctggtcctgg cgcaccgggg tcgtcaccat cgtgagcgtc aactgatcca
     9361 ggggaccgac catgattcgc cgaatcggca ctatcacgat caccctcgcg gccgctgccg
     9421 cctgcgcagc cgccgcgacc ggcacggcgg gagcagccgc cgcaactgcc tccggcaaca
     9481 aaggctctta tggtcccgac gtctcggcca ccgggcgcta cgtcgtcttc gcctccgatg
     9541 ccacgaattt agttgcgggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     9661 aaccacgcta cggacatctt cctgcgcgac ctgcccggca ccatccgacg cgtttccctc
     9721 atgcccacag gtgaccaact cagcaacgcc gccagccagg cgacgatcag caacgacggt
     9781 gcgcgtgtgg gcttcatggg cctgtccggc tcgagcgacg cgatcttcca gtggtcccgc
     9841 cgcctccagc gcacgacgat cctcagcgag gaagcatccg accatcaagt cgggtcacct
     9901 gtcacgagca atgggggggt cagctccgcc ggcgaggaga ccaacttcga ctgttggaac
     9961 agtgggatct ccgtgacggc gtcgccgaag tccgagtcgt tctacatcga cgacgattgt
    10021 ggcagcacgg tggattacgg ctacgacgtc agccgctggg gcgcgatggc aagcatcgcc
    10081 tatttcgact actcgatcaa cgccacgctg ctttacgtcg tagacgacta cttcgagctg
    10141 tcgtaccccg accggtatcc cccgctcgct cgcaaacggg tcaacgcgag cgcagtgtcg
    10201 atggcggccg acggatcgcg cgttgggtat gcgacgacga tcggtggcat ctcacagatc
    10261 cagctgtgga actggaagac cggcgccacc gcgacggtca gcaccagctg atgggcagcg
    10321 cggcacgccg agcgtgattc cgatgacttc cgcgtgccgg aaaggacggg ccactctagg
    10381 ctcgaacgta tgacgttctc catcgttgcc cgcgagggcc gcgccttcgg ggtggccgtg
    10441 gccagcaagt tcctcgccgt gggcggggtc gtcccggcgg cgcgcctggg caccggggcg
    10501 atcgcgaccc agtcgtatgc gcgggtgtcg ttcaaggccg acctgctcag ccatctggca
    10561 cgcggcaaca gcgcgagccg cgcgttggcg gccgtgacgg cggacgatct cgaggcgatg
    10621 aagcggcagg tgggcgtcgt cggcagcaag gacgccgcga ccttcaccgg cagcggctgc
    10681 cacccgtggg ccggcggcac gagcggcgag ggcagcaatg gcgatgactc ggcctacgcg
    10741 atccagggca atgtcctgac cggtccagaa gtcatcgccg agatggagcg cgccttcctc
    10801 gacagcgccg ggcagtcctt cacccgccgc ctgctcgcct cactcatggc aggggacgcg
    10861 gcgggcggcg actcccgggg tcgccagagc gccgcgctgt atgccgtagc ccccaaggcg
    10921 ggctacgacc gctccggcgt gctcgccgat ctgcgggtcg acgacaaccc ggacgcgatc
    10981 aacgaactgg cccgcatcca cgacctgcac gaactgtatt tcggtgcgcc ggaagacgtt
    11041 caaccgctca tcggtgatct cgaactcgag gtgcgctccc gcctcgacgg cctcggctac
    11101 cgcggccggc ccctcgcgga cgacctcgcc ctgtgggccg gcgaggcgaa cctcgagacc
    11161 cgcctcacgc gtgacggcat cgacggccgc gtcctggagg aactgcgcaa ggccggctga
    11221 ccccgggcag cccccctgtg gcgcgcgttt cgtgttctca gctggggtcg gatgtcacaa
    11281 gatgggcgat cacggcgggg tcatacccca actcgtgacg ccagcagggg gtcctcgtcg
    11341 caaagcgggg tatccggcgg cgtgcgtacc ccgacttgtg acagccgccg tcgtgggtcg
    11401 cgtcgatcgg cgtcagccgg cgcgcagggc ggctcagccg gcagtcagcc agcgctcaca
    11461 gccggccacc gcatacctcg ggcgagcagc gcttcggcga ctcggcggtt gagtgcaggg
    11521 acgtcgtgca ggtccgcggc gatgacgacg atgacgcgcc agccatccgc ctccagcgcc
    11581 tcccggcggc gcaggtcctt gcgccactgg cgccgctcga ggtgcacctc ggcgccggcg
    11641 tggcgcgagg gccgtgcgca gatcggccgg cgacgcgggt ccgtcgctga ggctcatcag
    11701 agcgtcggcg atgatcagcg catcgatttc gtgcagtcgc gggtcgctca gcgcggtggc
    11761 ccaggcggcg accggtgaga ccacgcgaaa ctctcccagg ggccaattgc ccgagccggt
    11821 gacgtgtcgg ccggatcccg cggacccggc accggatggc cgacgtgccc gccacgagcg
    11881 gatgcaacgg gtcgacggcg agcggcagcg gcaagtcgtc gtacccgcac gcgctgactc
    11941 cttcgagcac caggtccggg acgagccagg ctgccgcagc agcggtcgtg gccacgtcgt
    12001 cgggcaggtg cgccggcacc cgcaccccgc gaaacggcgc ccgcacctgt gacgaacgca
    12061 accggtcgca cgtcgggctg tctgcatcgc atgatcgccc acctcgggac acacccttgc
    12121 tttacagtcg ccgaatgcgc ttgatcccgc cgaccacccg cctgcaccgc tcctatctgg
    12181 gcggcgtcga tgagttcgcg ggggctcgcc gcgacgggga cggcctgtgg cagtgggtcg
    12241 aggacggagt gacccacgag atcaaccgcg aggagatgga gtccgaggac gggttcgccc
    12301 ggttcgtcgc ccgccgcacc gaccccgacc gggatctgct gcccggatgg gtgccgtgca
    12361 ccttcttctg gatggagcag gacggcgagt acgccggctc gctcgccttg cgccacgaac
    12421 tcaacgagtt ccttgcggcc gaaggcggtc acatcggcta cagcgtccgt ccgtcggcgc
    12481 gccggcgcgg tcacgcgacc gaggcactgc gccagtcctt gccccatgca cgcgctctcg
    12541 gcctcgaccg ggtcctggtc acctgcgacg agaccaatat cgcctccgcc cgcaccatcg
    12601 aggccaatgg cggagtcctg gcgaagaccg tcgaggccgc cagcgacgga cgcctcgtcc
    12661 gccactactg gatcgcccta taacgtcgcc ggtatgccgc tcatccgtga ggcccgcccc
    12721 gccgacgtcc cgcgcatcct cgccctcgtg cgcgatctcg cgcactacga gaaggaaccc
    12781 gacgccgtcg aggccaccga ggcggacttc acgcacgccc tcttccccac cgagggcgcg
    12841 cccacggcat acggccatgt cgccgaagtg gacggcgaga tggtggcgat ggcgatctgg
    12901 ttcctcacgt tctcgacgtg gaccggccaa gcgggcatcc acctcgaaga cctgtatgtc
    12961 gctccggcac aacgcggttc gggtctcggc acggcactgc tcacccggct cgcacaggtg
    13021 tgcgtcgaac ggggctatcg gcggctggag tgggcggtgc tgacgtggaa caccccggcc
    13081 atcgagttct acgacgcgct cggggcggag ccgatgacag cgtggaccga ctaccgtctc
    13141 gacggggccg ccctgcagcg cgtcggcgcg cactaaggtc gaagaagcag cacccgatcc
    13201 acgcaggagg cccacgatga ccacgacccg cgccctgacc gtgaccgcca atggcggcag
    13261 cgcggtcctc gctgtgcagc atgtcgacgt ccccgacccg gggccgggtc aggcccaggt
    13321 caaggtcgcg gccgccggcg tcaacttcat cgacgtctac caacgacagg gtgtctatcc
    13381 gatcccgacg ccgttcacgc tcgggatgga gggcgccggt gaagtggtgg ccgtcggcga
    13441 gggtgtcgac gacgtcgccg tcggcgatcg tgtcgcgtgg gcgatgcacc agggcacaag
    13501 cgcaggattg gcgaatatcg ctgctgccca actcattccg gtgcccgatg ggctggactt
    13561 caaggtcgct gccgcggccg ccctgcaggg gatgaccgcg cactatctcg tcaattccac
    13621 gtatgccgtc cagcccggcg atgtcgcgct cgtccacgcc gccgcgggcg gggtcggcca
    13681 actcctggtg cagatgatca ccgccaaggg cggcacggtc atcgccaccg ccggcggccc
    13741 ggagaagctc gccatcgccg agaggctcgg cgcgagcacg ctgatcgact accgcagcga
    13801 ggacgtggcc gcggcggcca gggcagccac cgatggcacg ggtgtgcatg tggcgtatga
    13861 cggcgtcggc aaggacacct tcgacgcctc cctggcctcg ctgcgccccc gcggcatggt
    13921 cgtgctcttc ggcggcgcga gcggccaggt gccgcccttc gacctgcagc ggctcaacgc
    13981 cgccggttcg gcctatgtca cccgcccctc actcgcgcac tacttgctca cgcgcgacga
    14041 actgctgtgg cgcgggcgcg aggtcttcgc ggccctggcc gacggctcac tggagctgga
    14101 ggtcggcggc gagtggcccc tcgaggaggc cgccgcggca tacgacgccc tcgagggacg
    14161 gcagagcacc ggcaagctcc tcctcacccc gtgacccgct accgcgatcg acatctcgac
    14221 gggggtcagc tggtctccca gagggcggcg atggcggtgt tgaggatgcc gaggacgccg
    14281 gcgtagtcga tgaggttgct gtcgcccgag cccttgcggg ccttcgcggc ggccatctcg
    14341 gcggcggcgg cgcaggcgag ggcgacggtg agtttgacgc cgaccttggc gtggttgagc
    14401 gggtcgttct gcgactcgtg gatgccgacc agggcgaggc cggtgaggat ctggaaacgg
    14461 gcaccccata ccaccaccga gagcccgcgg cctgactcga cgcccctggc gcgcaacgcg
    14521 atccagccac cgacgatgca ggccatgccg atgaggtgga cgagcacgag gacgttgatc
    14581 aggatctcca tgacggcact ctaaacgagc aactgcaggg ttccggtcag gtcctcgggt
    14641 atctcgagca gccactcccc cgtgccgcgc gcggtccacg cgggcccgcc ggccgggtat
    14701 acctccacga cgtcggcgtc gacccacacc tcggcgtcgg tgagttggag actcagcgca
    14761 gggtcgctgc tgctcgtcag tcgcccggcc gggacgtcgg cgcgcgccgc gccgggcagg
    14821 gtatgccgcc cgctccccac ctcctgcccc gcaccggatc cggtgagggc ttgcgccgcg
    14881 acggagtcga tccgactgac cagggccccg tcctgccagg cgagccggcg gggcagcgtc
    14941 aggcagccgg ccaccggtac gtcggggtcg tgcggcgtcc gggacgggat ccacccgatg
    15001 agcatcggct cctcgtcgtc ggtcatcact tgcggcgcat agaaattggg cccggtgtcg
    15061 acaagccccc acgcgatggc agccagttgc ggtccggcct cgacccggac ccgccggtcg
    15121 gccccgtagc tcgccgcgtc gatgtcgacc gcgacccaga ccaccggccc gggaggggcg
    15181 ccatgggtgc agagcgacag gatgagcacc gtcactccgt cgacggagca cagctgcggg
    15241 cactcccaga cgtcggacgg cggcagtgtg cggcagacgg gcgcgtcggc gcgggcgacc
    15301 acaccgacat accgccacgc cgtgatctcg ctggcgtcga agaggagcag ggccgcggtg
    15361 ccgtcgtgca gaccggcacc cacgagcgcg aaccgccttg cctcccagtc gaagacgaat
    15421 gggtcgcgca tctctcggac accgggcggc ggctcggcga cgacctgtgg agcgccccag
    15481 ctgagcaggt ccggggagcc ccagcgcagg catacggtgg cgtcggcagc cgaggcgacg
    15541 accccggaat agacgaccgc cgggcggtcc agtccgggca cgaagacccc cgaccagcac
    15601 cccccggcgt ccggacctcc cgtctgcggg ccgaaggcga ccggatgatg ttcccagaca
    15661 acgagatccg cgcttgacgc gtggccccag tggatatcgc cgtgcaccgg gccggcggga
    15721 ttgtgctgat agaagacgtg ccagcgtccg tcgtgaaagg tcagtccgtt ggggtcgttg
    15781 atccagccgg acgccggccg caggtgatag ctcgagagca cccgcgacag cctagatgag
    15841 cttgcggccc atggcccacg tggtcagctc gtgccgggag gagagctgga gcttgcgcag
    15901 caccgaagac acgtgcgtct caacggtttt gaccgagatg aacagttcgc gcgcgacttc
    15961 cttgtattgg tagccgcggg cgatcaggcg catgacctcg cgctcgcgct gggagagccg
    16021 gtccaattcc tcgtcgacct cggcgatctc accggcggag gcgccgaagg cgtccagcac
    16081 gaaaccggcc aggcgcggcg aaaagaccgc gtccccgtct gcgacgcgcc ggatcgccgt
    16141 ggccaggtcg gtgccggtga tggtcttggt gacatagccg cgggcaccgg cgcggatgac
    16201 ggcgatgacg tcgtcggcgg cgtcggagac ggacagcgcg aggaagcgga ccggctggcc
    16261 gtgcaggctg tcctgggcga cgcagccggc gagcacgtcg acacccccgc ggccgttgcc
    16321 gccgggcagg tggacatcga gcagcacgac atcggggtgg gtcgcgtgga tgactttgat
    16381 cgcctcgtcg acgtccgggg cctcgccgac gatctccagc gccgtgccgg cgacctcgcc
    16441 gagttcggcc cgcacgcccg tgcggaacat cttgtggtcg tcgacgatca ccacgcggac
    16501 agggggtttc ggcgtggcgg gcttaggcgt cgtggtcgcg gtcatcgcgg gcctccggtc
    16561 tcgggtgtcg gcagggtcag ggtccattcg gtgccggggt cgaggcgacg caacttggcc
    16621 gtgccgccgg cgcgggtcat ccggccgatg atcgactcgc gcacgccgag ccggtcctcc
    16681 tcgatggcgt cgaggtcgag tcccggcccg tggtcgcgca cgaacgcctc gacctcgccc
    16741 ggcccggcct cgacatagac ggcgatcggc ggagcgccgt gtcgcgcagc gttgttgagc
    16801 gcctcgcgca gcgcccgcac gagagcgagc acgtggtcgt cgacctcgcg gtcgccggtg
    16861 acgacgacgt cgatcggtat gccgtgcgcc acctcgatct cgtcgaccac ccgggcgatc
    16921 gctcccgaga gggaggtctc cgggtcaccg tccgcgccgt agagataggc ccgcagctcg
    16981 cgctcctgga cgcgtgcgag ggtcgcgacg cgggcagggt cgcccgattg gcgttggatg
    17041 agcgccagcg tctgcagaac cgaatcgtga aggtgcgcag caatatccgc gcgctcgtcg
    17101 gcgcggatct gggcgctgcg agcctcggcg ctgtcgctga gcaggcgcag ccccagcggc
    17161 gcgagcagca gcagcatgcc ggcgagcacg acgatggtcg cgacagcgat gtcggacgcc
    17221 gcgccgacgg actggcctcg gctcagcacg acgatggcgc cgacgacgac ggcaagggcc
    17281 ccggcaccca ggcgggcgag cgcgggccac cccgagcgcc ggccgagcca ccgatcacgt
    17341 tgtgccgcat cggcgctcgt caggccgagc acggcgatcg cgccgattgc cgcgagtggc
    17401 acgatcaggc tcagccgcgg gccggccgcc cgcgtgatgc cggtcatcat cccgagagcc
    17461 atgaggccga gcccgaagag cacggcaccg cgccgcatcg gtcgcgatgc gggtgacagg
    17521 gcaggatcgg cgacccccga cgccgccgcc gggtcgacgg tctgcacccg cgtgtcctcc
    17581 tgcggcgtga gcgcccacag cagcacatag gcgaccaggc ccggccccga gaagccgagc
    17641 gccgccatac cgatccgcac ccacaccacg gggaggtgca ggtgctcggc cagcccggcg
    17701 cagacaccgg ctatccatcg ctcaccggac gggcgcacca tccgcggccg cgtcgccgcc
    17761 ggggcggcgg tcgcgggggc gtacggcatc ggcataccag aagtctgacc catccggcgg
    17821 gcgagatggc gcacccagcc accgaatggg gcccgcctca gggacacgcc agggccgacc
    17881 cgcatcgcgc ccccgcgcgc gcgagggcag gctgggacca tgaccacacc gccgccccct
    17941 gcccccgcgc cgggaccgtc gtcgtcctcc gcgccggggc gcgagccgat cgtcgacgag
    18001 ttcacgtcgg cagtcgcccg gctcggcatc cggcgcacca cggagggctg ggtgggcggc
    18061 gtgtgcgacg gcatcgcgtt gcgctggagc atcgacccgg tcgtggtccg ggtggcgtgc
    18121 atcgtgctgg cgctcttcgg cggtttcggg atcgccgtgt atgccgcggg ctggctcctc
    18181 ctgcccgcgc cggacggccc gagcctgctg agccggatct ccgcgtccgc cgggcacacg
    18241 gtcgcgctcc tggccctggc cggcctcgcc ctggtcgtga tcgtcttcag cggctcgggc
    18301 ctgtacgacc cgcaggggtg ggggctcagt cagctgctgc tcctgctggt catcggcggg
    18361 gtcgggtatg ccgcgctgcg ctcccgcccc gggccgggca ccgctccgcc gcccccggcc
    18421 cagcaggccc cgcccggcgc ggctggatca cctggcgtgg ctggatcatc cggcgcgact
    18481 ggatcgtccg gtgcgactgg gttaactggt gcagctggat catctgttgc ggctggatcg
    18541 agcggcgggg ctggatcacc cggcggggca ccggaaacgc acccgacgcc cctgtggccg
    18601 ccggtgcacc gcgtcccggc aacccccggc ggcgccggag catccgacgt cgagcacacc
    18661 ctgcccgacc cggtctggcc cgacccggtc tggccggacc atggttcgcc gggtcctggt
    18721 tcgccgggtc ctggttcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
    18841 cttcccacga ccgccagccc gcccacaaca cccagttccc ccacaacacc cagctccccc
    18901 acaacaccca gctcccccac aacacccagc tcccccacaa cacccatcgc gcgcggccca
    18961 ctggcgaccg agggcagccc gcctgctccg gcgctgccgc gcagttggct cgaggcaccg
    19021 gccggggaga acggttcggc gacatccggt tcggggcggg gaagttccgc cccgacgcac
    19081 ccggaggggc agaccgaaac cgttgccgca cagcgacttt acaagcgtgg acccgcggtc
    19141 ttcaccatgt tctccggggc gctgctcctc ggtgttgcgg tcctgacgat catcgcccaa
    19201 gtgcgccgcg aaccgatcgc gttcgcgaca agcgcgccct acgtcctcgt cgggatcggc
    19261 ctgttcatca cggctgtcgg ggccctcgca gcccttggtc gccggcagcc gcccgccagg
    19321 tgagccaccg gtcggcggcc agcaacggcg cacgctggtg aggggcgagg gcgtcgacgt
    19381 cgagccacgc cgcttcgctc gtggtgccgc cctggtcgtg gacgacgagc gggcccggct
    19441 ccgggacggt cgcggcatac accagccgca ccacctgata gtcctcccag gtcccgtccg
    19501 gcgcctggcc gacccagtgc gaggtgagga gatcgagcag ccggatctcc cccaggtact
    19561 ggccggtctc ctcccacacc tcgcggcgca gcccggtggc cgggtcctca cccgggtcga
    19621 ccccaccacc gggcagggtc cagcggtggg ccgccccccg cacgtccgcc gcaagtcgcg
    19681 tggcgagcac gaggccgtcg cggacgacca cggcatacgc cgcaagtcgt tgcacgacaa
    19741 cggcatcgac gccctccggc ggccgctcgc cgctcgtcgc gggcacccag gtcgtatgcc
    19801 gctcctcccc cacctcggcg acgacgctca cctggatggt gacgccctcc tcatcggaag
    19861 gcgtgaacgc ctgccggacg cccgtcatgc tccagccgcg ccggtgcagc tcgtcactgg
    19921 ggtccgcgcc gtgcgggaga acgacgtcga cgacctcgtc tccggccagg gtgacgccga
    19981 cgatacggat gccgcggctc atcgcgttgc tcccgcccga cggtattccg cgagaacgca
    20041 ttcgacgata tgcgaccagt cctcggccgg cggagtggcg agattgtgag cgcggatccg
    20101 ttggcgcagc gcgtcgtgcg cgaccaggcg gcgggtcgcg gcgaccagac cggcatcgtc
    20161 ctccgccagc agtccgttga cgtcgtgggt aatgaagtcg gccacgccgg aggagccgat
    20221 cgccacgacc ggcagcccgg cggtgcgggc ctcgagggcg gcgatgccga aggcctccat
    20281 ccgcgtcggc gcgaggtaga cgtccgccgc ggcatacgcc gccgcgagcg cggggcgggt
    20341 cacgcgaccg ggcaggtgca gccagccgcg gtcgcgggag gacgggcgca accgggcggg
    20401 ggcgaggagc gggccgtcgc catagacggt cgcctccacc cggtcggggt cggcgtcatg
    20461 gacctcgtcg agcatcgcca acagggcgag cacccgcttg cgcggggcaa atcgcatcgc
    20521 ggccaccacc cgcacgcgcc cgctgtcgcg cgcgggcggc ataccgtcga gacgccacga
    20581 cgcagcgtcg attccgttgg gcagcacggc gatcgcgtcc ccaccaactc cgtcgtccac
    20641 tccggcggcg cggcccacga gccggcccgc catgcgcgac acgcccgaga gcgcggcacc
    20701 gcgggcccgc caccgggccg ccgtgcccag ccgccggtgc gcggcgatcg ccgcttcgcc
    20761 gaggacgcag tgccacgtga tcgccgtcgg caccgcgaga tcgagggcca ggttcgccat
    20821 gtcggcggcg aacggcgaga ccacgcccat gtgcacgtgg gcgacgtcga acgtgcgcag
    20881 ttcgtcgcga atgatgctgg gcgccaccgg attcaccggc agcgcgaacg gcaagggcag
    20941 cgtgacccgg tgcacggtca cccccgagtc cgccgcgacc gtgacattgc gcagccgcag
    21001 gcggggccgg ctctccgggg ttgccgtata gacgtgtacc tcgtgccccg ccgcagccag
    21061 ctgccggccg aggtcgcgca cctgggtctc gatcccgccc agccgcggcg ggtagcaatc
    21121 ggagagcagc gcgatcttca tggcccccac agcttgccac gaggcaccga ttgcgagcgg
    21181 gagcgcgcgg catacgggag gatgggggca tgaccacgag ccggccgtcc ggggggaggc
    21241 gcacgctcgt ggcctttcac gcccaccccg acgacgaggc gctgctcacc gccggcacga
    21301 tggccaaggc ggctgcggca gggcaccggg tggtgctcgt gctcgccacc aatggtgatc
    21361 tgggactgac ggattcggcg actcgggcgc gcgacctggg cgcgcaacgg ctggccgagg
    21421 cgcgacggag cgcagacgag ctcggggtgg cgcgcgtgga gtggctcggc tacgccgact
    21481 cggggatggg ccccgagctg tatgccgatc cgcccggccg gacccgcttc gtgcgggcgc
    21541 ccgtggccga ggcggcgcag cggctcgccg tgctgctgcg cgaggaatcg gccgatgtgc
    21601 tgttgtcgta tgacgcgcgc ggcggctacg ggcaccgtga ccacgtgcgg gttcacgagg
    21661 tcgctgcggc ggcggcacgc ctcgccggca cgccccgggt gctggaggcg acgatcccgc
    21721 gagatctgct cgccgcggcg gtgcgcctgt ccgccagggt ctatcgctat ccgcccgaat
    21781 tcgacattga gggtttctgc tcggccttca gtgcccgccg tgacatcacc catcggatcg
    21841 acgtgctgcg ctttgcggca gccaagcgcg cggcgatgcg cgcgcacgca tcgcaggcgc
    21901 tggccgacgg ggccgaccgc acgctggcgg cgttcctgcg gatcccgcgg ccggcatacg
    21961 acctgatctt cggccgggag tggttccgca acccggcgca cccggccggc gcgggcgtcg
    22021 cacgcgacat cttcgcgggg ctgccatgag caccgctcgc gagacggctc gcgattccgc
    22081 ggcccggccc cacttcggcc gcacgcaact cttgcaggcc gctgcgggcc tggccctctc
    22141 ggccgccctg ctgatctggg gcctgccgcg cttcgcccag acgagttggc acgaggtctg
    22201 ggccgtgctg accaccatca ccgtgggcaa cgccctgatc ttcctcgggc tggtgctcct
    22261 cgggttgtgg agctacaccc tgaccctgtc ggcgtcgatg ccggggctga gccatccgcg
    22321 ggccctggtc atgaatgtct gcggatcctc ggtgagcaac ctgctccccg ggggcggggc
    22381 ggtgggactg gcggcgacct acgcgatcgc ccgatcgtgg ggcatctcgc ggcgcgtggt
    22441 gtcgacgtcc gcgatcgtga ccggggtgtg gaacacgctg gcccggatcg cgttgccgct
    22501 catcgccatt gccgcgctga ccatcggccg ggtcgggatg acaccggtgc tgcgcgatgc
    22561 cgcgatcgcg gcgatgatca gcggcaccgt cgtcgtggtc ctcttcgtgg gggcgctggc
    22621 ttcggagcgg gtggcgcggc gggtcgggcg cggcctcgac cggctgttgg cgccgttgac
    22681 caggcggacc aagcgcacga tgacgatcga cgcgctggtg accgacctgc gctcgcgcat
    22741 cctgcacatc gtcgccaagg gctggctgcc catgacgctg ggcctcgtcg gctacttcgg
    22801 cttctactac ctcaccttcg tcgcgatcat gcgcaatacc ggtgtcgagc tcttccacgg
    22861 cgaactgttc gccgcgttcg ccatcggccg gctgctcacc gccgtgggga tcacccccgg
    22921 cgggatgggc gtgaccgaga ccggcaccgc cgccgccctc gtggcctggg gcgccgcccc
    22981 cgccgaggcg gcggccggcg tcgtcctctt ctcgatcttc acccacctga tggaagtccc
    23041 cctcggcgcc ctcggctggc tggcctggag cgtcataccc aaagccaccg agcccgacct
    23101 gaccgacccc gaccccccgc ccgacctccc ctgatcccgg tgcacaccaa cgggatccga
    23161 gtaacctctg ctagcagagg ttccttgggc ttggtccggg gttcggggcc ggggcgggta
    23221 tggcgcgggg gtcagccgac gagccggcgg acctggcggg tgcgggcctg gtcggcga
//
LOCUS       DAOPJG010000023        23229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2327_length_23229_cov_7.305774, whole genome shotgun sequence.
ACCESSION   DAOPJG010000023 DAOPJG010000000
VERSION     DAOPJG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 23229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23229
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_23229_cov_7.305774"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKU75_03925"
     CDS             complement(<1..603)
                     /locus_tag="PKU75_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HPF80056.1"
                     /translation="MTAIEADLRRGNFRIGDRVQLTDPKGRMHTITLEAGKEFHTHRG
                     FIRHDDLIGAPDGSTLTNTVGIEYLAIRPLLADYVMSMPRGAAVVYPKDAGQIVMMGD
                     IFPGAVVVEAGVGSGALSMSLLRAVGESGRVLSFERRTDFATIAQANARAFFGSDHPA
                     WTVTVGDLVESLPAAVEPGTVDRVVLDMLAPWECLDVVGDA"
     gene            complement(624..1721)
                     /locus_tag="PKU75_03930"
     CDS             complement(624..1721)
                     /locus_tag="PKU75_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPF80057.1"
                     /translation="MTSQPGQPPAPGIKVATIAGVPVYIGRSWGLLALIIVALIGPQL
                     QPDLGGRAYLVAIGYALLLLLAVMVHEGAHALGARSFGFPVHRVVADLWGGHTALDVS
                     RSTPVRSATIAVVGPLSNLVLSAVGYGLAAVLDPGIAHGLAGLFGYLNLLLALFNLLP
                     GLPLDGGQLVESLVWKLTGDRHKGRVAAGYCGMAVTLGVLYWFVGRPLLRGDQPSLFS
                     IGWAFLICMFLWQGARSAIASGQVGGALSRVRVVDVMTPVAPVPYDAALTQLPTGVPP
                     VVVDQSGTPVALVDAAALQAVPPQAAPHTSVSSVSRPQPPGWVLDLDPNAPVTELFAP
                     LRDLGTGVVAIRQNGRLLGVVTVDAVNNSLS"
     gene            1794..5510
                     /gene="metH"
                     /locus_tag="PKU75_03935"
     CDS             1794..5510
                     /gene="metH"
                     /locus_tag="PKU75_03935"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPF80058.1"
                     /translation="MPFSPDATSTLTELLRRRILFLDGAMGTMLQRHRLGEADFRGER
                     FADWGSDVIGNNDLLSLTQPHLIREVHDQYLAAGANLISTNTFNAQRISMADYGMQEL
                     SAELNLASARLAREAVDAALATDPHTPRFVIGSLGPTNRTASISPDVSDPGARNVAYD
                     ELVAAYHEQAAALVEGGADVLAIETIFDTLNAKAAIFAVETLFEEQGRRWPVIISGTI
                     TDASGRTLSGQVAEAFWNSVRHVRPIAVGFNCALGAAQMRPFIADLSRVADTFVSCYP
                     NAGLPNAFGEYDETAGETAAALIEFAQAGLVNLVGGCCGTTPEHIAAIAQGVGGIAAR
                     PVPQVPVAMRLSGLEPLTITEDSLFVNVGERTNITGSARFRKLIQAEDYPAALSVARQ
                     QVEAGAQVIDVNMDEGMIDGVAAMDRFLKLIASEPDISTVPLMIDSSKWEVIEAGLKC
                     VQGKPIVNSISMKEGVEPFLEQARLCRKYGAAVVVMAFDETGQADTLERRKEISKRAY
                     ELLTQEVGFPAEDIIIDPNIFAVATGIEEHAAYGTDFIEATRWIKQNLPGVLVSGGVS
                     NVSFSFRGNNPVREAIHAVFLYHAINAGMDMGIVNAGQLVVYDQIDPELRERIEDVVL
                     NRRADATDRLLEIAEKFNVAGAEDDSSAPEWRTLPVAERITHALVKGIDDFVEADTEE
                     LRTEIAARGGRPLEVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAYLIPF
                     IEAEKQPGDASHAKGRIVTATVKGDVHDIGKNIVGVVLQCNNYEVHDLGVMVPAQKIL
                     DTAKELEADAIGLSGLITPSLDEMVNVASEMERQGFEIPLLIGGATTSKAHTAVKVDQ
                     RYHGPVVWVKDASRSVPVVSQLLSDDHRPKLVASIADEYDTIRTRHAAKSGERPLLSL
                     EQARAAAPEIEISAETVATPKQAGIHRFTDYDLAELREYIDWTPFFAAWEMRGRFPDI
                     LNNPASAAAARKLYDDAQAMLDQIVEQKWLRANGITGIFPAARVGDDTVVYTDDTRTE
                     VRMTLHHLRQQGQHREGVPNRSLADFIAPQDSGIPDWIGAFAVTAGLGSQERVMAFKA
                     EHDDYNAILLEALADRLAEAFAERLHERVRTELWAYAPDETLDNQALIEERYRGIRPA
                     PGYPACPDHTEKAVIWELLDVQEETGIELTEHMAMWPGAAVSGLIFAHPQAQYFVVGR
                     LGRDQVEEYAGRKGWDLRTAERWLSPNLGYHPEE"
     gene            complement(5554..6918)
                     /locus_tag="PKU75_03940"
     CDS             complement(5554..6918)
                     /locus_tag="PKU75_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HPF80059.1"
                     /translation="MLSRAAHAAPPLGAPLFEPDIISSRKGVLEAELVAQAATRVVGG
                     IPRPVLTFNGTVPGPTLFVEPGDLLRIRLVNQLPEGTNIHTHGLHVSGMGNADNMMIH
                     VMPGESFDYEIPLRSNATEGMNWYHPHVHHHTGQQIFSGMFGTLFTQPKGEGRIPKDI
                     SRDRVITLSTSFWTPDGDIASGTRTSQARQIRLVNGEINPTIDIAPGETQRWRFVNTS
                     MNHVFRLTLDGHTMTRVAADGMPYTAALELTELRLAAGQRADVLVTGGEAGMHALRVL
                     PFDFGFGVVSVEDTLATVVCGGEGVTPTPVNLNKLLAPRTDLRKFKVDKRRTLTFTTA
                     GGFAIDGKPFDPNRIDQVCEIGALEQWTIKNPTGLTHPFHIHVNPFQVVSVNGTSVDT
                     RSYEDTVLVDKLGGEVTFLTRFEHFLGRVIYHCHFGNHSDLGMMGQAWVVPRGCGTSP
                     IVPV"
     gene            7180..9672
                     /gene="treY"
                     /locus_tag="PKU75_03945"
     CDS             7180..9672
                     /gene="treY"
                     /locus_tag="PKU75_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HPF80060.1"
                     /translation="MSSTPSRNPLPRRDHRPGLTRATPISTYRLQINGEFGFDAAAAV
                     VPYLAELGVSHLYLSPILEPEPGSTHGYDVVDHDALNSEAGGREAFERLSDAARAAGL
                     GIIVDIVPNHMTVPQETYLNSQLWDVLRHGRASQYADWFDVDWEAEDFKILMPILGAD
                     LDTALTNGELAVAHDGGADSTETVLRYYDHELPVRPGTESLDLPALVDAQAYRLAHWE
                     DGQTDLNYRRFFDVTTLQAVRVEEDDIFDATHELLLELYREGRIDGFRIDHPDGLADP
                     GGYLGRLAHRTGNAWVVVEKILEGHEELMPQWACAGTTGYDALLRVQQVLTDPTGTET
                     LDAMWAEIAAPERVDLGDVIIAAKLQVVEEVQAAEVNRLLRLVERVLPDTDLEAARRA
                     LTALLVSMDRYRAYLVAGQRPARSQLVVLAQAEGRARDRLDAGDHPVLGQITQLARGG
                     RPAGGLSDPDAHEAIAEFVTRFQQTCGPVMAKGIEDTAFYRWLRQTGANEVGGDPGTL
                     SIEVDEFHAYAEERLEHWPLTMTTLSTHDTKRSEDVRARLAVLSERAQDWQDWFAEAR
                     RMSARLRNGLVDPETEYLIWQTLVGTWPMTPERMSTYLLKATREAKTHTAWVDGDEPY
                     EEAIVAFATALIEDPDVGAHIQRWVDQTAESSRAFSLAQKLIQLTMPGVPDLYQGSEY
                     ALLTLVDPDNRGAVDYDEHRIRLEALSEGALPQDLSEAKLRVVTTALRLRREHPEWFV
                     GRTADYLALDTGSPNAIAFARGGTDGPAAVTVAAIAPHRLRNSGGWQDEHLDLPAGDW
                     RDLLSGNEFTSVDGIPTGDVLGEQPVALLVRR"
     gene            9669..11507
                     /gene="treZ"
                     /locus_tag="PKU75_03950"
     CDS             9669..11507
                     /gene="treZ"
                     /locus_tag="PKU75_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HPF80061.1"
                     /translation="MTGEPVRIAVWAPRAGAMEIALGETPQTEPMQTEPGQTEPRQTE
                     PMQAGAGGWWSWEGDPAAYGEHLDYAFIVDGAQPPLPDPRSAWQPHGVHGPSRHFDTT
                     VFEWTDDAWRGTRSGAGVLGGVVYELHIGTFTPEGTLDAAVEHLDHLVELGVDLVQLL
                     PVAAFPGRWGWGYDGVALYAVHDGYGGPAALQRFVDAAHGKGLGVCLDVVYNHLGPSG
                     NYLSRFGPYFTDRHHTPWGEAVNLDGPDSRTVRDFVIGNATRWMRDFHIDALRLDAVH
                     ELRDDSDMHLLAELSDAVGRLAAELGRPLELIAESDLNDTVMVTSTALGGLGMTAQWD
                     DDVHHALHTVLTGEIHGYYSDFGGGAQGHEEQDALAVLAKVFTRGFLHDGTFSTFRGG
                     PWGKPVATESFDARRLLGYLQTHDQVGNRALGERITALLPPGRQHIGAALYLLGPFTP
                     MIFAGEEWATSSPWLFFTSFEDADLAEAVRLGRREEFAGHGWAAETIPDPQAVGTRDE
                     SVLRWGELHGTGGSMFQWYRDLIALRRKHIDGPTHLADVVIEADETARLVRMQHAGLS
                     VIANLGEEEASFEMAEDPGVELLSNGAVTVEGRQLTLAPDAVVILG"
     gene            complement(11515..12177)
                     /locus_tag="PKU75_03955"
     CDS             complement(11515..12177)
                     /locus_tag="PKU75_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPF80062.1"
                     /translation="MTDRPALPAAVLWDMDGTLVDTEPYWIAAEHEIVAEHGGQWSEE
                     LAHQLVGNPLLVSARFILDNSPVTMSPEELVEELLRRVVLQVRDHIPWRPGAKELLFE
                     LVDLGVPCALVTMSWQSLAHAVTAALPDGVFAAVVTGDVVTHGKPHPEPYLVACAALD
                     AAPGECVAIEDSATGVRSAVAAGVPTLAVPHVIALPDIDGAVHVASLEGMRVGELLRL
                     FG"
     gene            12274..13131
                     /locus_tag="PKU75_03960"
     CDS             12274..13131
                     /locus_tag="PKU75_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPF80063.1"
                     /translation="MIELEDLPPLNDPIMIVACEGWNDAGEAATGAVDHLAELWDAEP
                     VAALDPEDYYDFQVNRPRVLLDKGRRRIQWRTTRILLAQPEDIDRSIILVSGIEPSFH
                     WRGYTVEIMEFAQAAGATTFVTLGALMADVAHTRAIPVTATSEDDATMHRFDLEPSGY
                     EGPTGIIGVLSDAATQSGLTAISCWAAVPHYAGHAPSPKATLALLTTLASLFDLPIDT
                     TDLPELAQQWEAGINSLAESDEEIAEYVEALEKAQDTAELPEASGDAIAREFERYLRG
                     HGPDDQSEG"
     gene            complement(13247..14491)
                     /gene="mshC"
                     /locus_tag="PKU75_03965"
     CDS             complement(13247..14491)
                     /gene="mshC"
                     /locus_tag="PKU75_03965"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HPF80064.1"
                     /translation="MKTWPEVTLPAIPGTGRPLRVHDTASDALVTIEGSDGVARLYVC
                     GITPYDATHLGHAATYVTFDLINRAMRDSGLQVRYVQNVTDVDDPLLERAARDNRDWR
                     ELAGAEIGLFREDMTALGVLPPDELVGVVESIDEIAQAVRQLVAAGAAYDLPNDGLDS
                     GDIYLDLTRLDEFNDVSGFSRDQMMEVIVDRGGDPDRPGKRDPLDPLLWRAERAGEPA
                     WHVDGLPAGRPGWHIECTAIARDHLGLPIDIQGGGVDLVFPHHDMSATQACAVGEHFA
                     RAFVHQAMVGLHGEKMSKSKGNLVLVSRLRADGVDPMAIRLLLLAQRYDTPWDYTDDL
                     LEAATARLATWRAALSVNEAPDSAPVIAAVRDALADNLDTPAALAAVDAWASAVAADA
                     AGMPHAPGILARTLDALLGVRV"
     gene            complement(14515..15369)
                     /locus_tag="PKU75_03970"
     CDS             complement(14515..15369)
                     /locus_tag="PKU75_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HPF80065.1"
                     /translation="MPVLRGAARRDRAHLPPGERLPALSEDPLTTAELHVIGQLTEAS
                     NATWLTRLGDPERGPLAVYKPVAGERPLWDFPDGTLAARERAAYLVSELGGWHVIPRT
                     VLRDGPAGPGMVQEWIGSIESENEGVIHLIGIGSDPAGLRPVLRATDDRGMPVLLAHE
                     DTADVRSLAVLDAVLNNTDRKGAHAFRCAGQLYAVDHGVTFHHEPKLRTVLWGWGGKR
                     LPENEIQRLRSLLIALVEPGEQAEEIRSHLTVREHEALLARVDHLIGTGRHPRPDGAW
                     HAIPWPPI"
     gene            complement(15297..15833)
                     /locus_tag="PKU75_03975"
     CDS             complement(15297..15833)
                     /locus_tag="PKU75_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HPF80066.1"
                     /translation="MSLAQYDPPERFVAGTVGPPGNRTFFLQAREGIRITTVSVEKVH
                     IQAIADRISDLLEATASLPTQTPDADNEPLETPFEDEFRVTTMSLAWDTDRDVVVLEC
                     HDRDPDGDEEEQAQARAAGLPADIQSMRVILSSPMAAEFVRRCKALVSAGRPPCPFCG
                     GPLDATGHICPRANGYRR"
     gene            15930..16616
                     /locus_tag="PKU75_03980"
     CDS             15930..16616
                     /locus_tag="PKU75_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF80067.1"
                     /translation="MVRLLLVEDDETVRETTASYLRDAGHEVAAVADGDGALASYAQS
                     PADLVLLDLMLPGTPGLDLCRHLRAMRADLPVIMVTARSQEHERVQGLALGADDYVTK
                     PFSLRELDLRIRSVLRRSAPPAPEPARRELVDGDLRLDLAARTALRRGDPLPLTAREF
                     DLLAWFIGHPARAWAREELMANVWGWEYGDASTVTVHVRRLREKVEPDPGAPVRLVTV
                     FGRGYRWDPA"
     gene            16613..17623
                     /locus_tag="PKU75_03985"
     CDS             16613..17623
                     /locus_tag="PKU75_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPF80068.1"
                     /translation="MSPAGSAIAISAAVTGVVALLGALVLLTVSRRRAAWAALAAPLV
                     VVISLAAGVAAATRAMAIGIHDYRTLIFVLLAGAPMAVLVGAAVARRIFVQERAAAAE
                     RAERDRAASVEADRRELIRWLGHDLRTPLSGIRVLAESLREQESGATPAAAALDGILR
                     ESDRLDDMVADIAELSRLQGEMSATVRREAVHLADLVSDAAACVRPLAEAARVELTPG
                     ALTDVRVTVDTAALTRAVTNLLRNAVQHTPPGGRVLISVQSPAQVLVADGCGGVPEAD
                     LAHVFEPGWRADAARTSGIAGMGLGLAITREIARSHGGDVDAANLPDGSGCVFTLRLP
                     AA"
     gene            complement(17585..18319)
                     /locus_tag="PKU75_03990"
     CDS             complement(17585..18319)
                     /locus_tag="PKU75_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HPF80069.1"
                     /translation="MPTLLLIRHGRTTANAAGTLAGWSPGIGLDEVGRDQAARLAERL
                     AALPVCRVVASPLQRTLETAAPLAAAWGLEVVQDEGVGECRYGAWTGRPLKDLAQEEL
                     WKVIQRAPSAAVFPAGDDYPHESLAAMSARAVETVRRADADVLAAYGPTAVWAAVSHG
                     DVIKAIVADALGMHLDQFQRIVVDPASVSVIRYPVGQAMVLGVNGSGTDLEGIVPTQQ
                     EAQSDEGQVGGGAGSGGGQPQGEDAP"
     gene            18344..19321
                     /gene="corA"
                     /locus_tag="PKU75_03995"
     CDS             18344..19321
                     /gene="corA"
                     /locus_tag="PKU75_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPF80070.1"
                     /translation="MIVDQAKYVDGVRQPCGDLSDELAALRSASDRPDFLWIGLKNPT
                     DAEFELVREELDLHPLAVEDVLKGNQRAKIERYDGTTFVVLKTLRYVEETSDVETGEV
                     MAFVGDRFLLTIRHGEAAALQGVRHRLEADPEMLALGPQGALHAVIDQVVDGYTAIDD
                     ELAKDLETIEEAVFAGDRSVHSQTVYNLKREVLEFKRAAVPLHKAISVHFSPASTMAD
                     SEIRLLYRDVLDHLSQSIDHIESYERLLTDVLHAYLARVGVRQNEDMRKISAWVAIAA
                     MPTMIAGIYGMNFEHMPELGWRFGYAFALAIMFTVCLLLYRAFRRSGWL"
     assembly_gap    19341..19440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19568..20419)
                     /locus_tag="PKU75_04000"
     CDS             complement(19568..20419)
                     /locus_tag="PKU75_04000"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPF80071.1"
                     /translation="MLSYFDAVVLGIVEGLTEFLPVSSTGHLTVAEKMLGLRVDDKAV
                     TAYTVIIQFGAIVATFIYFAKKIARLFMAWVRGLTNADARGHDYTLAWAVIVGSIPVG
                     IAGFLGKDLIAGPLRNLWVVAAALVLWSAVMVWAEKRHDALEAAGDQRGEGQVTMRDG
                     IMIGLMQCFSLIPGVSRSGATISAGLLRGIDRVTATELSFFMAIPALTAGGVFEALQE
                     RDTLGNLGFGQMAVGMLVAFVVAYASIAWLLKFVATNSLMAFVSYRVVAGLALAAALG
                     VGWIAAT"
     assembly_gap    20688..20787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20788..20993)
                     /locus_tag="PKU75_04005"
     CDS             complement(<20788..20993)
                     /locus_tag="PKU75_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80072.1"
                     /translation="MADPRAALAALTAAFERHLEACAQRRGEDDPAVVAAYDDLADAF
                     EEYDDALLEAYGEMTPLDVYAGPD"
     gene            complement(21043..22350)
                     /locus_tag="PKU75_04010"
     CDS             complement(21043..22350)
                     /locus_tag="PKU75_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF80073.1"
                     /translation="MNAADEVARICQELIRIDSSNYGDGSGPGEAEAAAYVVAQLREV
                     GLDPQVVESEPGRASVFVRIPGSDSALPALCVHGHLDVVPAHAADWQVEPFSGEERDG
                     CIWGRGAVDMKGMDAMMLAVVRDLVRTGTSPRRDLVIGFFADEEAGGVYGSQWVVANH
                     PEFFDGVTEAISEVGGYSVTLRRADGSQQRAYLLQTAEKGILWLRLTARGRAGHGSVP
                     NDENPIVRLAQAIARIDAHEWPQEFIPSVQELLDGLSRITGIPNTGDLEPLLQHLGGA
                     AGFVRGTLQDTANLTMLDAGYKHNVIPQDAHASVDCRFLPGHDETVMKTLKELCGDHV
                     DIDIHHEDISLDSPFEGGLVEAMIAALQAEDPGAEVLPYCLSGGTDNKALSRLGITGY
                     GFAPLQLPADLDFAPMFHGIDERVPVESLRFGTRVLHRLLTSY"
     gene            complement(22385..22867)
                     /locus_tag="PKU75_04015"
     CDS             complement(22385..22867)
                     /locus_tag="PKU75_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80074.1"
                     /translation="MTTRRTKLAAIAMAGMLALAACGGGDSESSSTTPAAAGSTSASA
                     PASTSAASSSSAGSASGSGSYDAAGCAAVGQSLMDIANVALDSLTGDFSQADFDKAFP
                     GGVVDKFPKDLQAGYTELETLAKDLIGKSQTEAMEASQAFSTKISDYTNQVSKACIPS
                     "
     gene            complement(22974..>23229)
                     /locus_tag="PKU75_04020"
     CDS             complement(22974..>23229)
                     /locus_tag="PKU75_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPF80075.1"
                     /translation="QWMIANPDRVPGTGSRWSAGKDLSFVPLRPERVLEVGYDHMEGR
                     RFRHTAQFKRWRPDRDPASCTYDQLEEVARYDLAAILRPE"
BASE COUNT         3615 a         7955 c         7998 g         3461 t
ORIGIN      
        1 ggcgtcaccg acgacgtcca ggcactccca cggcgcgagc atgtcgagga cgacccggtc
       61 gacggtgccc ggctcgaccg ccgccggcag gctctcgacg aggtcgccga cggtgaccgt
      121 ccacgccgga tggtcgctgc cgaagaacgc gcgcgcattc gcctgggcga tcgtggcgaa
      181 gtcggtccgc cgctcgaagg agagcacccg ccccgactcc cccaccgcgc gcagcaggga
      241 catcgacagc gccccggatc cgacgccggc ctcgacgacc acggcgccgg ggaagatgtc
      301 gcccatcatc acgatctgcc cggcgtcctt gggatagacg accgccgcgc cgcgcggcat
      361 cgacatcaca tagtcggcga gcagtgggcg gatcgcgaga tattcgatgc cgaccgtgtt
      421 ggtcagggtc gagccgtcgg gcgcgccgat caggtcgtcg tggcggatga acccgcggtg
      481 ggtgtggaac tccttgccgg cttccagcgt gatcgtgtgc atccgcccct tcgggtcggt
      541 caactgcacc cggtcgccga tgcggaagtt gccgcggcgc aggtccgcct cgatcgccgt
      601 catggggcga aatgctacgt cggctacgac aggctgttgt tcaccgcgtc gacggtgacc
      661 acgccgagga gtcggccgtt ctggcggatc gcgacgacgc cggtgccgag gtcgcgcagg
      721 ggcgcgaaga gttcggtcac gggagcattg gggtcgaggt cgaggaccca gccgggtggc
      781 tgcggccggc tgacggagga gacggaggta tgcggtgcgg cttggggcgg cacggcctgc
      841 agggcggcgg cgtccaccaa ggcgaccgga gtgccgctct ggtcgacgac caccggcggg
      901 accccggtgg gcaactgggt gagggcggcg tcgtacggca ccggggcgac gggggtcatg
      961 acgtcgacca cccgcactct cgacagcgcc ccgcccacct ggccggacgc gatcgccgag
     1021 cgcgcgccct gccacaggaa catgcagatc aggaacgccc acccgatcga gaacaacgac
     1081 ggctggtcgc cgcgcagcag gggccggccg acgaaccagt acagcacgcc gagcgtgacg
     1141 gccataccgc agtagcccgc cgccacgcgg cccttgtgcc ggtcaccggt cagcttccac
     1201 accagcgact ccacgagctg gccaccgtcg aggggcaacc caggcagcag gttgaacagg
     1261 gcgagcagca ggttgagata gccgaacagg ccggccaacc cgtgcgcgat tcccggatcg
     1321 aggacggcgg cgaggccgta accgacggcg ctgaggacca ggttggagag cgggcccacg
     1381 acggcgatcg tcgcgctgcg cacgggggtg gagcgactga cgtcgagcgc ggtgtgcccg
     1441 ccccacaggt cggccaccac gcggtgcacg gggaagccga acgaccgcgc ccccaaggcg
     1501 tgggcaccct cgtgcaccat caccgccagc agcagtaaca aggcataccc gatggcgacg
     1561 aggtaggcgc ggccgccgag gtcgggctgc agctgcgggc cgatgagggc gacgatgatc
     1621 agcgcaagca ggccccagga cctgccgatg tagaccggca ccccggcgat cgtcgccacc
     1681 ttgatgccgg gggcgggcgg ttggccgggt tgcgaggtca cgcgccgaac ctatctggcc
     1741 gcgccgggca aagttcgcgc gagcaggcgc cggcgtgacg gatactgcta cccatgccgt
     1801 tctcccccga cgccacgtcg acgctcaccg aactgctcag gcggcgcatt ctcttcctcg
     1861 acggcgcgat gggcacgatg ctgcagcgtc accgcctcgg tgaggccgac ttccggggcg
     1921 agcgcttcgc cgactggggc agcgacgtca tcggcaacaa cgacctgctg tcgctgaccc
     1981 agccacacct gatccgcgag gtccatgacc aatacctcgc cgccggggcc aatctgatct
     2041 ccaccaacac cttcaacgcc cagcgcatct cgatggccga ctacggcatg caggagctga
     2101 gcgcggaact caatctcgcc tcggcgcgcc tggcgcgcga ggcggtggac gccgcgctgg
     2161 cgaccgatcc gcatacccct cgcttcgtga tcggctccct cggcccgacg aaccgcaccg
     2221 cgtcgatctc ccccgacgtg tccgatccgg gcgcgcgcaa tgtggcgtat gacgagttgg
     2281 tggcggcgta tcacgagcag gcggcggcgc tcgtcgaggg cggcgccgac gtcctcgcga
     2341 tcgagacgat cttcgacacc ctcaacgcca aggcggcgat cttcgccgtc gagacgctct
     2401 tcgaggagca gggccggcgc tggccggtca tcatctccgg gaccatcacc gacgcctccg
     2461 ggcgcaccct ctcgggtcag gtcgccgagg cgttctggaa ctccgtgcgc catgtgcgcc
     2521 cgatcgccgt ggggttcaac tgcgcgctcg gggcggcgca gatgcggccg ttcatcgccg
     2581 acctgtcgcg ggtcgcggac accttcgtct cgtgttaccc gaatgcgggg ctgcccaacg
     2641 ccttcgggga gtatgacgag acggccggtg agacggcggc cgcgctgatc gagttcgctc
     2701 aggcgggcct ggtcaacctg gtcggggggt gctgcggtac gacgcccgag cacatcgccg
     2761 cgatcgccca gggcgtcggt ggcatcgctg cgcgtccggt cccgcaggtg ccggtggcga
     2821 tgcggctgtc cggcctggaa ccgttgacga tcaccgagga cagcctcttc gtcaacgtcg
     2881 gcgagcggac gaatatcacc gggtcggcgc gcttccgcaa gctcatccag gccgaggact
     2941 atccggccgc cctctcggtg gcgcgccagc aggtcgaggc cggtgcccag gtcatcgacg
     3001 tcaacatgga cgagggcatg atcgacggcg tcgccgcgat ggaccgcttc ctcaaactca
     3061 ttgcctccga accggatatc tccaccgtgc cgctgatgat cgactcctcc aagtgggaag
     3121 tcatcgaggc cgggctgaag tgcgtgcagg gcaagccgat cgtcaactcc atctcgatga
     3181 aggagggcgt cgagcccttc ctggagcagg cgcggctgtg ccggaagtat ggcgcggcgg
     3241 tggtcgtcat ggccttcgac gagaccgggc aggccgacac cctcgagcgg cgcaaggaga
     3301 tcagcaagcg ggcctacgag ttgctgacgc aggaggtcgg tttcccggcc gaggacatca
     3361 tcatcgaccc caacatcttc gcggtggcga cggggatcga ggagcacgcg gcatacggca
     3421 ccgacttcat cgaggcgacc cggtggatca agcagaacct gcccggcgtg ctcgtctccg
     3481 gcggtgtgtc gaatgtgtcc ttcagcttcc gcggcaacaa cccggtccgc gaggcgatcc
     3541 atgccgtctt cctctatcac gcgatcaacg ccggcatgga catgggcatc gtcaacgccg
     3601 gccagctggt cgtctacgat cagatcgacc ccgagctgcg cgagcggatc gaggacgtcg
     3661 tgctcaaccg ccgggcagac gcgacggacc ggctgctgga gatcgccgag aagttcaacg
     3721 tcgctggggc cgaggacgat tcgtcggcac cggagtggcg caccctcccc gttgccgagc
     3781 ggatcacgca cgctctcgtc aaggggatcg acgacttcgt cgaggccgac accgaggagt
     3841 tgcgcaccga gatcgcggct cgcggcggcc ggccgttgga ggtcatcgaa ggtccgctga
     3901 tggacggcat gaatgtcgtc ggcgacctct tcggcgccgg caagatgttc ctgccgcagg
     3961 tcgtgaagtc ggcccgggtg atgaagaagg ccgtcgccta tctcatcccg ttcatcgagg
     4021 ccgagaagca gccaggagac gcctcgcacg ccaaggggcg catcgtcacc gcgacggtca
     4081 agggcgacgt ccatgacatc ggcaagaaca tcgtcggcgt tgtcttgcag tgcaacaact
     4141 acgaggtgca cgacctcggg gtgatggtgc cggcgcagaa gatcctcgac acggccaagg
     4201 aactggaggc cgacgcgatc ggcctgtccg gcctgatcac cccctcgctc gacgagatgg
     4261 tcaatgtggc gtccgagatg gaacgccagg gcttcgagat ccccctgctc atcgggggcg
     4321 cgacgacgtc gaaagcgcac accgccgtca aggtcgacca gcgctatcac ggcccggtgg
     4381 tctgggtgaa ggatgcctcg cgctccgttc ccgttgtctc ccaactgctt tcggacgacc
     4441 accgcccgaa gctcgtggcg tccatcgccg acgagtacga cacgatccgc acccggcacg
     4501 cggccaagtc cggagagcgg ccactcctga gcctggaaca ggcgcgcgcc gcggcacccg
     4561 agatcgagat ctccgccgag accgtggcga cgccgaagca ggccggcatc caccgcttca
     4621 ccgactacga cctggccgag ttgcgcgagt acatcgactg gacgccgttc ttcgccgcgt
     4681 gggagatgcg cggccggttc ccggacatcc tcaacaaccc tgcttcggcc gcggccgcgc
     4741 gcaagttgta tgacgacgct caggcgatgc tcgaccagat cgtcgagcag aaatggttgc
     4801 gcgccaacgg gatcaccggg atcttcccgg cagcccgggt cggcgacgac acggtcgtct
     4861 ataccgacga cacccgcacc gaggtgcgca tgacgctgca ccacctgcgc cagcaggggc
     4921 agcaccgcga gggcgtgccc aaccgctcac ttgccgattt catcgcaccc caggacagcg
     4981 gcataccgga ctggatcggc gccttcgccg tcacggcggg tctgggttcg caggaacgcg
     5041 tgatggcgtt caaggccgag cacgacgact acaacgccat cctgctcgag gccctcgccg
     5101 accggctcgc cgaggcgttt gccgagcgcc tgcacgagcg ggtccgcacc gagctgtggg
     5161 cctacgcgcc cgacgagacc ctcgacaacc aggccctgat cgaggagcgc taccgcggca
     5221 tacgacccgc ccccggctat cccgcctgcc ctgaccacac cgagaaggcg gtcatctggg
     5281 agctgctcga cgtccaagag gagacgggca tcgagttgac cgagcacatg gcgatgtggc
     5341 ccggcgccgc tgtgagcggg ctgatcttcg cccacccgca ggcacagtac ttcgtcgtcg
     5401 gtcgcctcgg gcgcgaccag gtcgaggagt atgccggccg taagggctgg gatctccgca
     5461 ccgccgagcg ctggctctcc cccaacctcg gctaccaccc ggaggagtga aacgcgcggc
     5521 cctgacgcac ggttgggccg cgcgcccaga gcctcagacc gggacgattg ggctcgtgcc
     5581 gcacccgcgt ggcacgaccc aggcctgacc catcatgccg aggtcggagt gattgccgaa
     5641 gtggcagtgg tagatcaccc ggccgaggaa gtgctcgaac cgggtcaaga aggtgacctc
     5701 gccgccgagc ttgtcgacca gcacggtgtc ttcataggag cgggtgtcga ccgacgtgcc
     5761 gttgacggag accacctgga aggggttgac gtggatgtgg aaggggtgcg tcaggccggt
     5821 cgggttcttg atcgtccact gctcgagcgc gccgatctcg cagacctggt cgatccggtt
     5881 ggggtcgaac ggcttgccat cgatggcgaa ccctcccgcg gtggtgaacg tcagcgtgcg
     5941 ccgcttgtcg accttgaact tgcgtaggtc cgtccgcggg gcgaggagtt tgttcaggtt
     6001 caccggtgtc ggggtcaccc cctcgccgcc gcagaccacc gtcgccaggg tgtcctcgac
     6061 cgagacgacc ccgaagccga agtcgaaggg caggacgcgc agggcgtgca tgcccgcttc
     6121 gccaccggtc accaggacat cggcacgctg accggcggcg agccgcaact cggtcaactc
     6181 aagtgcggcg gtgtagggca ttccgtcggc cgccacgcgc gtcatcgtgt gaccgtcgag
     6241 ggtgagccgg aagacgtggt tcatcgaggt gttcacgaac cgccaccgct gcgtctcacc
     6301 gggggcgatg tcgatcgtcg ggttgatctc gccgttgacc agccggatct gacgagcctg
     6361 ggaggtgcgg gtgcccgagg cgatgtcgcc atcgggggtc cagaaactcg tcgacagcgt
     6421 gatgacgcga tcccgactga tgtccttcgg gatgcggccc tcccccttgg gttgagtgaa
     6481 gagcgtcccg aacatgccac tgaagatctg ctgaccggtg tggtgatgga cgtgcgggtg
     6541 gtaccagttc atgccctcgg tggcgttgct ccgcaagggg atttcgtagt cgaagctctc
     6601 ccccggcatg acatggatca tcatattgtc ggcattgccc atgccggaga cgtgcaggcc
     6661 gtgggtgtgg atattggtgc cctccggcag ttggttgacc agccggatcc ggagcaggtc
     6721 acccggttcg acgaagagcg taggaccggg cacggtgccg ttgaaggtga gcacgggacg
     6781 cggtatgccg cccacgactc gcgtggccgc ctgcgccacg agctcggcct ccagcacgcc
     6841 cttccgcgag gagatgatgt ccggctcgaa gagcggtgcg cccaagggcg gtgccgcgtg
     6901 ggcagccctg ctgagcatcg ggacgcctcc ggctcccagg gcgaccccgc cggccgcgct
     6961 caggaggaca cggcgagaaa cgccagagcg ggtcggatca atcgttggca tcgggacctt
     7021 cctcatcatc ggttagctga accgatgacc cactccgccc gggaatacct caattacgca
     7081 aatgtgacat gccaaacacg ggatggcgcg cgccggccgt ccggccggct atcggcgctg
     7141 gacggtcgag acccgccgcg ttggtagagt cgggcagcga tgtcatcgac accttcgcgc
     7201 aatccgctgc cccgccgaga ccaccgcccg ggcctgaccc gggcgacccc gatctccacc
     7261 tatcgacttc agatcaacgg agagttcggg ttcgacgccg cggcggcggt ggtcccctat
     7321 ctcgccgaac tcggtgtcag ccacctctac ctgagcccca tcctcgagcc ggagccgggc
     7381 agcacccacg gctatgacgt cgtcgatcac gacgcactga actccgaggc gggcggccgc
     7441 gaggcgttcg agcgcctttc ggacgcagcc cgggccgccg ggctcgggat cattgtcgac
     7501 atcgtgccca accacatgac ggtgccacag gagacctatc tcaacagcca gctgtgggac
     7561 gtcctgcgcc acggccgcgc ctcgcagtat gccgactggt tcgacgtcga ctgggaggca
     7621 gaggatttca agatcctcat gcccatcctc ggcgccgacc tcgacaccgc cttgaccaac
     7681 ggcgaactgg ccgtcgctca cgatgggggc gcggacagca ccgagacggt gctgcgctac
     7741 tacgaccacg agctgcccgt ccggcccggc acggagagcc tcgaccttcc ggcgctcgtg
     7801 gacgcacagg cataccggct ggcccactgg gaggacggcc agaccgacct gaactaccgc
     7861 cgcttcttcg acgtcaccac cttgcaggcg gtgcgcgtcg aggaggacga catcttcgac
     7921 gccacccacg aactgctcct cgagctctat cgcgagggcc ggatcgacgg cttccggatc
     7981 gatcatccgg acggcctcgc cgatccgggc ggctatctcg ggcgcctcgc ccaccggacc
     8041 ggcaacgcct gggtggtcgt cgagaagatc ctcgagggcc acgaggagct gatgccccag
     8101 tgggcgtgcg caggcacgac cgggtatgac gccctgttgc gcgtgcagca ggtcctcacc
     8161 gacccgaccg gcaccgagac gctcgacgcc atgtgggccg agattgccgc gcccgagcgg
     8221 gtggacctcg gggacgtcat cattgccgcc aaactgcagg tcgtcgagga ggtccaggcc
     8281 gccgaggtca accgactgct gcgcctggtc gagcgggtcc tgcccgacac cgatctcgag
     8341 gccgcgcgcc gcgccctgac cgcgctgctc gtctcgatgg accgctaccg cgcctatctc
     8401 gttgcgggac aacgtccggc gcgctctcag ctcgtcgtgc tcgcccaggc cgagggccgg
     8461 gctcgggacc ggctcgacgc cggcgaccac ccggtgctcg gccagatcac ccagctcgcc
     8521 cggggcggcc gccccgccgg cggcctgtcc gaccccgacg cccacgaggc catcgccgag
     8581 ttcgtcaccc gcttccagca gacgtgcggc ccggtcatgg ccaagggcat cgaggacacc
     8641 gcgttctatc gctggctgcg ccagaccggc gccaacgagg tcggcggcga cccgggcacg
     8701 ctctcgatcg aggtcgacga gttccacgcg tatgccgagg agcggctcga gcactggccg
     8761 ctgacgatga cgacgttgtc cacccacgac acgaagcggt cggaggacgt gcgcgcccgg
     8821 ctggccgtgc tgtccgaacg ggcccaggac tggcaggact ggttcgccga agctcgccgg
     8881 atgtcggcgc ggctgcgcaa cggattggtc gaccccgaga ccgaatacct catctggcag
     8941 accctcgtcg gcacgtggcc gatgacgccc gagcggatgt ccacctatct gctcaaggcg
     9001 acccgcgagg cgaagacgca cacggcctgg gtcgacggcg acgagccgta tgaagaggcg
     9061 atcgtcgcct ttgccacggc gctcatcgag gaccccgacg tcggggcgca catccagcgc
     9121 tgggtcgacc agaccgctga atcatcgcgc gctttctcgt tggcgcagaa gctcattcag
     9181 ttgacgatgc ctggggtacc cgacctctac cagggcagcg aatatgccct gttgaccctc
     9241 gtcgatcccg acaaccgggg agcagtcgac tacgacgagc accgcatccg gctggaggcg
     9301 ctttccgagg gggcgctgcc tcaggatctg tccgaggcca agctgcgcgt cgtcacgaca
     9361 gcactgcggc tgcggcgcga gcaccccgag tggttcgtcg ggcgcacggc ggactatctg
     9421 gccctggaca ccgggagccc gaatgcgatc gccttcgccc gaggtggcac cgacgggccg
     9481 gcagccgtga cggtcgccgc gatcgctccc catcgactcc gcaactccgg cggctggcag
     9541 gacgagcacc tcgacctgcc tgcaggtgac tggcgggatc tgttgtccgg caacgagttc
     9601 acctcggtcg acggcatacc caccggggac gtcctgggcg agcagcccgt cgcgctgctg
     9661 gtgcgccgat gaccggtgag ccggtccgga tcgccgtctg ggcgccgcgg gccggggcaa
     9721 tggagatcgc cctcggggag acaccgcaga ccgagccgat gcagaccgag ccggggcaga
     9781 ccgagccgag gcagaccgag ccgatgcagg ccggcgccgg cggctggtgg tcctgggaag
     9841 gcgaccccgc ggcatacggc gagcacctcg actacgcctt cattgtcgac ggtgcgcagc
     9901 caccgctgcc cgacccacgc agcgcgtggc agccgcacgg tgtgcacggc ccgagccgcc
     9961 acttcgacac gaccgtcttc gagtggaccg acgacgcctg gcgcggaacc cgcagcggcg
    10021 caggcgttct cggcggcgtg gtctatgaac tgcacatcgg caccttcacc cccgagggca
    10081 ccctcgacgc tgcggtcgag cacctcgacc acctcgtcga gctgggggtc gacctcgtcc
    10141 agctccttcc ggtcgcggcc ttccccggcc gctggggctg gggctacgac ggcgtcgcgc
    10201 tgtatgccgt gcacgacggc tacggcggac cggcggccct gcagcgtttc gtcgacgccg
    10261 cccacggcaa aggtctcggc gtgtgcctcg acgtcgtcta caaccacctc ggaccgagtg
    10321 gcaactacct gagccggttc ggcccctatt tcaccgaccg gcaccacacg ccgtggggcg
    10381 aagcggtcaa cctcgacggc ccggacagcc ggaccgtccg ggacttcgtc atcggcaatg
    10441 cgacgcggtg gatgcgcgac ttccacatcg acgccctgcg cctcgacgcg gtccacgaac
    10501 tccgggacga ctccgatatg cacctgctcg cggaactgtc cgatgcggtg ggccgtctcg
    10561 ccgccgagct gggcaggccg ctcgagctga tcgccgagag cgacctcaac gacacggtca
    10621 tggtgacctc gaccgccctc ggcgggctgg gcatgacggc ccagtgggat gacgatgtcc
    10681 accacgcgct gcacacggtc ctgaccggcg agatccacgg ctactacagc gatttcggcg
    10741 gcggggcgca aggccacgaa gagcaggacg ccttggccgt cctcgccaag gtcttcacgc
    10801 gcgggttcct ccacgacggc accttctcca ccttccgtgg cgggccgtgg ggcaagccgg
    10861 tggccaccga atcgttcgac gcccggcgcc tgctcggcta cctgcaaacc cacgaccagg
    10921 tcggcaatcg cgctctggga gaacgcatca cggcgctgct gcccccgggg cggcagcaca
    10981 tcggggcggc gctctatctg ctcggaccat tcacgccgat gatcttcgcc ggcgaggagt
    11041 gggcgacctc gtcgccatgg ctgttcttca ccagcttcga ggacgccgac ctggcggaag
    11101 cggtccggct gggccggcgt gaggagttcg ccggtcacgg gtgggcggcc gagacgatcc
    11161 ccgacccgca ggcggtcggc acccgcgacg aatcggtctt gcgatggggt gaactccacg
    11221 gcaccggcgg cagcatgttc cagtggtatc gcgacttgat cgcgttgcgc cgcaagcaca
    11281 tcgacgggcc gactcatctc gccgacgtcg tcatcgaggc cgacgagacc gcacggctgg
    11341 tgcgtatgca gcacgctgga ctctcggtca tcgccaacct cggcgaggag gaagccagtt
    11401 tcgagatggc tgaggatccg ggggtcgagt tgttgtccaa tggtgctgtc accgtcgagg
    11461 ggcggcagct gacgctggcc ccggacgccg tcgtcatcct cggctagaac cgcctcagcc
    11521 gaacagcctc agcaactcgc cgacccgcat accctcgagt gacgcgacgt gaacggcgcc
    11581 atcgatgtcc ggcagcgcga tgacgtgcgg cacggccagg gtggggacac ccgcggcgac
    11641 cgctgaccgg acccccgtgg ccgaatcctc gatcgccacg cactcccccg gcgccgcgtc
    11701 gagggcggca cacgcgacga gatagggctc ggggtgcggt ttgccgtggg tgacgacgtc
    11761 gccggtgacc acggccgcga agactccgtc gggcagcgcc gccgtgacgg cgtgcgcgag
    11821 cgactgccag gacatcgtca ccagggcgca cggcaccccg agatcgacga gttcgaagag
    11881 caactccttg gcgccgggcc gccacgggat gtgatcgcgc acctgcagca cgacccgccg
    11941 cagcaattcc tcgaccagtt cctcggggct catcgtcacc ggcgagttgt cgaggatgaa
    12001 ccgtgccgaa accagcaacg ggttgcccac gagttgatgg gcgagctctt cgctccactg
    12061 gcccccgtgt tcggcgacga tctcgtgctc cgctgcgatc caatatggct cggtgtcgac
    12121 gagggtcccg tccatatccc acagcacggc ggcgggcagg gccgggcggt cggtcacgaa
    12181 aggtcctcat cggagacggc aggatcggtc cgagtgtacg gcgacggcag ggctctgacc
    12241 gtaatgccca tccgcacgcg atagcctgcc gacgtgatcg agctcgagga cctgccgccg
    12301 ctgaacgacc cgatcatgat cgtcgcctgc gaggggtgga acgacgcggg cgaggccgcc
    12361 accggggcgg tggaccacct cgccgagctc tgggacgccg agccggtcgc cgccttggac
    12421 ccggaggact actacgactt ccaggtcaac cggccccggg tgctgctcga caagggccgc
    12481 cgccggatcc agtggcgcac cacccgcatc ctgctcgccc agccggagga catcgaccgc
    12541 agcatcatcc tggtcagcgg gatcgagccg agcttccact ggcgcggcta cacggtcgag
    12601 atcatggagt tcgcgcaggc ggccggggcg accaccttcg tcaccctggg cgctctgatg
    12661 gccgacgtcg ctcacacccg cgccatcccg gtcaccgcca cctccgagga tgacgcgacg
    12721 atgcatcgct tcgatctcga acccagcggc tacgagggcc cgaccggcat catcggtgtc
    12781 ctgtccgacg ccgcgaccca gagcggtctg accgccatct cgtgctgggc cgccgtgccg
    12841 cactacgccg gtcacgcccc ctccccgaag gccaccctgg cgctgctgac gaccctcgcc
    12901 tcgctcttcg acctgccgat cgacaccacc gacctgcccg aactcgccca gcagtgggag
    12961 gccggcatca actccctcgc cgagtccgac gaggagatcg ccgaatacgt cgaggccctc
    13021 gagaaggccc aggacaccgc cgaacttccc gaggccagcg gcgacgccat cgcccgagag
    13081 ttcgagcgct acctacgcgg ccacggcccc gacgaccagt cggagggctg acccagcgaa
    13141 gtatcgacgc gacgaaggag cggagaactt cgtggcattt gctgacactt ttggggtccc
    13201 cgcgaagtat cgacgcgacg aaggagcgga gaacttcgtg gggtgtttag acgcggacgc
    13261 cgaggagagc gtcgagggtg cgggcgagga tgcccggggc gtgcggcata ccggcggcgt
    13321 cggcggccac ggctgacgcc cacgcatcga ccgcggccag cgcggcgggc gtgtcgagat
    13381 tgtccgcgag cgcgtcccgg accgcggcga tcaccggcgc gctgtccggg gcctcgttga
    13441 ccgagagcgc ggcgcgccag gtggccagcc gcgcggtggc ggcttcgagg aggtcgtcgg
    13501 tgtagtccca cggcgtgtcg tatcgctgcg cgagcagcag cagccggatc gccatcgggt
    13561 ccaccccgtc cgcgcgcagg cgcgagacga gcacgaggtt gcccttggac ttgctcatct
    13621 tctcgccgtg caggccgacc atcgcctggt ggacgaaggc gcgcgcgaag tgctcgccga
    13681 ccgcacacgc ctgggtcgcc gacatgtcgt ggtgcgggaa gaccaggtcc actccaccac
    13741 cctggatgtc gatcggcagg ccgagatggt cgcgcgcgat cgcggtgcac tcgatgtgcc
    13801 acccggggcg gccggcgggc aggccgtcga cgtgccacgc gggctccccg gcccgctcgg
    13861 cacgccagag cagcgggtcg agggggtcgc gtttgcccgg gcggtccggg tcgccgcccc
    13921 ggtcgacgat gacctccatc atctggtcgc gggagaaacc gctgacgtcg ttgaactcgt
    13981 ccagccgggt caggtcgagg tagatgtcgc cggagtcgag cccgtcgttc ggcaggtcgt
    14041 atgccgcccc ggccgccacg agctgccgca cggcttgggc gatctcgtcg atggattcga
    14101 cgacgccgac gagttcgtcg gggggcagga cgccgagggc ggtcatgtcc tcgcggaaga
    14161 ggccgatctc ggcgccggcg agctcccgcc agtcgcggtt gtcgcgggcg gcgcgctcga
    14221 gcagcgggtc gtcgacgtcg gtgacgttct ggacgtaacg cacctgcaga ccgctgtcgc
    14281 gcatcgcgcg gttgatcagg tcgaaggtga cataggtagc ggcgtggccc aagtgggtgg
    14341 cgtcataggg cgtgatgcca cagacataca gccgcgccac gccgtcgctg ccctcgatcg
    14401 tcaccaatgc gtcggaggcg gtgtcgtgca cgcgcagcgg gcgtccagtt ccggggatgg
    14461 cgggcagggt cacctcgggc cacgtcttca cgggccaact gtatgcggag gggtctagat
    14521 cggtggccag gggatggcgt gccacgcacc gtcggggcgg gggtggcggc cggtgccgat
    14581 caggtgatcg acgcgcgcca gcagtgcctc gtgttcgcgg acggtcaggt gggagcggat
    14641 ctcctccgcc tgctcccccg gctcgacgag ggcgatcaac agggaccgca ggcgctggat
    14701 ctcgttctcg ggcaggcgtt tgccgcccca tccccacagg accgtgcgca gtttgggctc
    14761 gtggtggaag gtcacgccgt gatcgacggc atacagctgc cccgcgcagc ggaaggcgtg
    14821 ggcacccttg cggtcggtgt tgttgagcac cgcgtcgagg acggcgaggg agcgcacgtc
    14881 cgcggtgtcc tcgtgcgcga gcaacaccgg cataccgcga tcgtccgtgg cacgcagcac
    14941 cggccgcagt cccgcgggat cgctgccgat cccgatgagg tggataacgc cctcgttctc
    15001 gctctcgatg ctgccgatcc actcctgcac catgcccggg cccgccggcc cgtcgcgcag
    15061 gacggtccgg ggtatgacgt gccagccacc gagttcgctc acgaggtatg ccgcccgctc
    15121 ccgggccgcg agcgtgccgt cggggaagtc ccacagcggc cgctcccccg ccacgggttt
    15181 gtagaccgcg agcgggccgc gctccgggtc accgagccgg gtgagccatg tcgcgttgga
    15241 ggcctcggtg agctggccga tgacgtgcag ctcggccgtg gtgagcgggt cctcgctcag
    15301 cgccggtagc cgttcgcccg ggggcagatg tgcccggtcg cgtcgagcgg ccccccgcag
    15361 aacgggcacg gcggccggcc ggcggacacc agcgccttgc aacggcgcac gaactcggcc
    15421 gccatcggcg agctgaggat cacgcgcatc gactggatgt cggcgggcag ccccgcggcc
    15481 cgcgcctgcg cctgctcctc ctcgtcgccg tcggggtcgc gatcgtggca ctccaggacg
    15541 acgacatccc ggtcggtgtc ccacgccagc gacatcgtcg tgacgcggaa ctcgtcctcg
    15601 aagggtgtct cgagcggctc gttgtcggcg tccggggtct gcgtgggcag cgacgcggtg
    15661 gcctcgagca ggtcgctgat ccggtcggcg atggcctgaa tgtgcacttt ctcgaccgac
    15721 acggtcgtga tgcgaatgcc ctcgcgggcc tggaggaaga aggtgcgatt gcccggcggg
    15781 ccgacggtgc cggcgacgaa gcgctccggc gggtcgtact gcgcaaggct cataccccga
    15841 ccctaacccg cgagagagtt cttaacagcg ccttacggcc ggtatgccgc gggcgggctc
    15901 gcgcgcgtca gcgccctact cttggggccg tggtgcgctt gttgctggtc gaggacgacg
    15961 agaccgtccg ggagacgacg gcgagctact tgcgcgacgc cggtcacgag gtggcggcgg
    16021 tggccgacgg cgacggcgcg ctggcgtcgt atgcgcagtc gcccgccgac ctcgtgctgc
    16081 tcgacctgat gctgccgggc acgccaggcc tggatctgtg ccgccacctg cgagccatgc
    16141 gcgccgatct gccggtcatc atggtcaccg cgcggagcca ggagcacgaa cgtgtccagg
    16201 gcctggcgct cggggcggat gactatgtca ccaaaccgtt ttcgctgcgc gagttggacc
    16261 ttcggatccg ttcggtgctg cgccggtcgg ccccgccggc acccgaaccc gctcggcggg
    16321 aactggtcga cggcgacctg cgtctcgacc tggcggcgcg cacggcgttg cgtcgtgggg
    16381 atccgctgcc cctgaccgcg cgagagttcg atctgctcgc ctggttcatc ggacatcccg
    16441 ctcgcgcgtg ggcgcgcgag gagctgatgg ccaacgtctg gggctgggag tacggcgacg
    16501 cctccacggt gacggtgcat gtgcgacggc tgcgggagaa ggtcgagccg gatccgggcg
    16561 caccggtccg gctcgtgacg gtgttcgggc gcggctatcg ctgggatcct gcgtgagtcc
    16621 cgccggctcg gcgatcgcca tctccgcggc ggtcacgggc gtcgtcgcgc tcctcggcgc
    16681 cctggtcctg ctgaccgtca gccggcgccg ggccgcgtgg gcggccttgg cggcacccct
    16741 cgtcgtcgtc atctcgctgg cggcgggagt ggcggccgca acgcgcgcca tggcgatcgg
    16801 catccacgac taccggaccc tgatcttcgt gctgttggcc ggtgcgccga tggcggtcct
    16861 tgtcggcgcg gccgtggccc ggcgcatttt cgtgcaggag cgggcggccg cggcggaacg
    16921 cgccgagcgg gatcgggccg ccagcgtcga ggccgaccga cgggagctga tccggtggct
    16981 cggtcacgac ctgcgcaccc cgctgtccgg catccgagtc ctggcggaat cattgcggga
    17041 gcaggaatcc ggggcgacac cggccgccgc ggcgctcgac ggcatcctcc gcgagagcga
    17101 ccggctcgac gacatggtcg ccgatatcgc cgaactgtcc cggctccagg gcgagatgtc
    17161 cgcgaccgtg cggcgcgagg cggtgcacct ggccgatctg gtctccgacg ccgcggcctg
    17221 cgtccggccc ctcgccgagg cggcgcgggt ggagttgacg ccgggcgccc tcaccgacgt
    17281 gcgcgtcacc gtcgacacgg ctgccctcac ccgggcggtg acgaacctgc tgcgcaacgc
    17341 cgtgcaacac actccacccg gcgggcgcgt cctcatcagc gtgcagtccc cggcgcaggt
    17401 tctcgtggcg gacgggtgcg gcggcgtacc ggaagccgac ctcgcccatg tcttcgagcc
    17461 gggctggcgg gccgacgccg cccgcacgag cggcatcgcc ggcatggggc tgggactggc
    17521 catcacgcgc gagatcgccc ggtcccacgg cggggacgtc gacgccgcga acctgccgga
    17581 cgggtcaggg tgcgtcttca ccctgcggct gcccgccgcc tgacccggct cccccaccga
    17641 cctgcccctc gtcgctctgc gcctcctgct gcgtcggcac gatcccctcg aggtcggtcc
    17701 cggatccgtt gacgcccaac accattgcct gtccgacggg ataccggatg acactcaccg
    17761 acgccgggtc gacgacgatc cgctggaact ggtcgaggtg catccccagg gcgtcggcca
    17821 cgatcgcctt gatcacgtcg ccgtggctga ccgctgccca caccgccgtc gggccgtatg
    17881 ccgcgagcac gtccgcgtca gcgcggcgca ccgtctcgac ggcgcgggcc gacatcgccg
    17941 ccaggctctc gtgcgggtag tcgtccccgg cggggaagac cgccgccgag ggggcgcgct
    18001 ggatgacctt ccacagctcc tcctgcgcga ggtccttcag cggccgaccg gtccacgcgc
    18061 cataccggca ctcccccacg ccctcgtcct gcacgacctc gagaccccac gcggcggcga
    18121 gcggcgccgc cgtctccagg gtgcgctgca gcggggaggc gacgacgcgg cataccggca
    18181 gcgctgcgag gcgctcggcc agccgtgccg cctgatcgcg gcccacctcg tccaggccga
    18241 tgcccggcga ccagcccgcg agcgtgcccg cggcgttggc ggtcgtgcgt ccatggcgga
    18301 tcaacaggag cgtaggcacg cggcaagtat gacaatggag cccgtgatcg tggatcaggc
    18361 gaagtatgtc gacggggtgc ggcagccctg cggggacctc agcgacgaac tcgcggcgtt
    18421 gcgcagcgcg agcgaccgtc ccgacttctt gtggatcggc ctgaagaacc ccacggacgc
    18481 ggagttcgaa ctggtgcgag aggaactcga cctgcacccg ctcgccgtcg aggacgtcct
    18541 gaaaggcaac cagcgcgcca agatcgagcg ctatgacggg accaccttcg tggtcctgaa
    18601 gaccctgcgc tacgtcgagg agacctcgga tgtcgagacg ggcgaggtca tggcgttcgt
    18661 cggcgaccgc ttcctgctga cgatccggca cggggaggcg gcggcgctgc agggcgtgcg
    18721 acaccggctg gaggccgacc cggagatgct cgcgctcggc ccgcagggcg ccttgcacgc
    18781 cgtgatcgac caggtggtcg acggctacac ggccatcgac gatgagttgg ccaaggacct
    18841 cgagaccatc gaggaggcgg tcttcgccgg tgaccggtcg gtgcattcgc agacggtcta
    18901 caacctcaag cgtgaggtgc tcgaattcaa acgggcagcg gtgccgctgc acaaggccat
    18961 ctccgtgcac ttcagcccgg caagcaccat ggcggacagc gaaattcgcc tgctctatcg
    19021 cgacgtcctc gaccacctgt cgcagtcgat cgaccacatc gagtcgtatg aacggctact
    19081 cacggatgtc ctgcacgcct acctggcgcg ggtcggcgtg cgccagaacg aggacatgcg
    19141 caagatctcc gcctgggtcg cgatcgcggc gatgccgacg atgatcgccg ggatctatgg
    19201 catgaacttc gagcacatgc cggagctggg ctggcgcttc ggctacgcct tcgccctggc
    19261 catcatgttc acggtctgcc tgctgctcta tcgggcgttc cgtcgctccg gctggctctg
    19321 agtcacccca caaagttctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 cgctcgcacg ctcgctccga tactttgcgg ggaccccgac atagtcaccc cacgaagttc
    19501 tccgctcgca cgctcgctcc gatactttgc ggggaccccg acatagtcac cccacgacgt
    19561 tctccgctca ggtggcggcg atccagccga cgccgagcgc ggcggccagg gcgaggccgg
    19621 cgacgacgcg atagctgacg aaggccatca gggagttggt ggcgacgaac ttcagcagcc
    19681 acgcgatcga ggcatacgcg acgacgaagg cgacgagcat acccaccgcc atctgcccga
    19741 aaccgaggtt gccgagggtg tcgcgctcct gcaacgcctc gaagacaccg ccggccgtca
    19801 gcgccgggat ggccatgaag aagctcagct cggtggcggt gacccggtcg atgccccgca
    19861 gcagacccgc cgagatggtt gcccccgacc gggagacgcc ggggatcaac gagaagcatt
    19921 gcatcaggcc gatcatgatt ccgtcgcgca tcgtgacctg gccctcgccg cgctggtcgc
    19981 cggcggcctc cagcgcgtcg tgacgtttct cggcccagac catgaccgcg ctccagagca
    20041 ccagcgcggc cgcgacgacc cacaggttgc gcagcgggcc tgcgatcagg tccttgccga
    20101 ggaagccggc gatcccgacc gggatcgatc cgacgatcac ggcccacgcg agcgtgtagt
    20161 cgtgaccccg cgcgtcagcg ttggtcaggc cgcgcaccca ggccatgaag agccgagcga
    20221 tcttcttcgc gaagtagatg aaggtcgcca cgatcgcacc gaactggatg atcacggtgt
    20281 atgccgtgac cgccttgtcg tcgacccgca ggcccagcat cttctcggcg accgtgaggt
    20341 gacctgttga ggagacgggc aggaattcgg tcaagccttc gacgatgccg agcacgacgg
    20401 catcgaaata gctgagcatg aagcagcaac ctcttggggc aggggacgac ggggatcgcc
    20461 gatggcgacc ggtggagctc cggcctcacc aggagaccag aggaccctga gagccgactg
    20521 agaattctac cggctcgggc gtcaagcgct ggcgacgctc agttcgggcg ggtctcgccg
    20581 tcgtcctcga tctcgtcgaa atcctcgtcg tcgagcccga ggtagcgctg gtcggcgtcg
    20641 gcgtcgtcgt cctcttccgc cgcgtcgtcc tcctcctcgt cgtcctcnnn nnnnnnnnnn
    20701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnntcg tcggggccgg cgtagacgtc gaggggcgtc
    20821 atctcgccgt aggcctcgag cagcgcgtcg tcatactcct cgaaggcgtc cgccaggtcg
    20881 tcgtatgcgg cgaccaccgc cgggtcgtcc tctccgcgcc gctgagcgca tgcttccaag
    20941 tgacgttcga aggcggcggt gagggccgcg agggcggcgc gggggtcagc catggcccaa
    21001 ccgtagcgcc tccgacgggc ccgctgacta cgcggtggcg cgctagtagg acgtcaggag
    21061 ccggtgcagc acgcgggtgc cgaaacgcaa cgattccact ggcactctct cgtcgatgcc
    21121 gtggaacatc ggcgcgaaat ccaggtcagc aggcaattgc agaggggcga atccatagcc
    21181 ggtgatcccg agccgggaca gggccttgtt gtcggtgccg ccggagaggc aatagggcag
    21241 gacctcggcg ccggggtcct cggcctgcag cgcggcgatc atggcctcga ccagcccacc
    21301 ttcgaagggg ctgtcgaggg agatgtcctc gtggtggatg tcgatgtcga cgtgatcacc
    21361 gcagagctct ttgagggtct tcatgacggt ctcgtcgtga ccgggcagga agcggcagtc
    21421 cacggaggcg tgggcgtcct gcgggatgac gttgtgcttg tagccggcgt cgagcatcgt
    21481 taggttcgcg gtgtcctgca acgtacctcg gacgaatccg gcggccccgc cgaggtgctg
    21541 taggaggggc tcgaggtcac cggtattcgg aatgccggtg atgcgcgaga gcccgtcgag
    21601 gagttcctgg acggagggga tgaactcctg cggccactca tgcgcgtcga tgcgggcgat
    21661 ggcctgggcg agccggacga tggggttctc gtcattgggc accgacccgt ggccggcgcg
    21721 accgcgcgcg gtcaggcgca gccagaggat gcccttctcg gcggtctgca gcaggtaggc
    21781 gcgctgttgg ctcccgtcgg ctcggcgcag ggtgacactg tagccgccaa cctcgctgat
    21841 ggcttcggtg acgccgtcga agaactccgg gtggttggcc acgacccact gtgagccgta
    21901 gacgccgccg gcctcctcgt cggcgaagaa gccgatgacc aggtcgcgcc gggggctggt
    21961 gccggtgcgc accaggtccc ggacgacggc cagcatcatc gcgtccatgc ccttcatgtc
    22021 gacggcgccc cgtccccaga tgcagccgtc gcgttcctcg cctgagaagg gttcgacctg
    22081 ccagtcggcg gcatgggcgg gcacgacgtc gaggtgcccg tgcacgcaca acgccggcag
    22141 ggccgagtcc gaaccgggga tccgcacgaa gacactcgcg cggcccggct cggactcgac
    22201 cacctgcgga tcgagaccga cctcgcgaag ctgggccacg acatacgcag ccgcttcggc
    22261 ctccccaggc ccgctgccgt cgccgtagtt gctggagtcg atccggatca gttcctgaca
    22321 gatccgggcg acctcatcgg ctgcgttcat gctggctcct gggtcgaggt ggtcatcgat
    22381 gtcgtcagga cgggatgcag gccttgctca cctggttggt gtagtcgctg atcttcgtcg
    22441 agaaggcctg gctggcctcc atggcttcgg tctgcgactt gccgatgagg tccttggcga
    22501 gggtctccag ttcggtgtag ccggcctgca ggtccttggg gaatttgtcg acgacgccgc
    22561 cggggaaggc cttgtcgaag tcggcctggc tgaaatcccc ggtcagcgaa tcgagcgcga
    22621 cattggcgat gtccatgagg gactgcccga cggccgcgca ccctgccgcg tcatacgatc
    22681 ccgagccgct cgccgaaccg gccgaactcg aggatgccgc ggaggtggac gccggcgcgc
    22741 tcgcgctcgt ggagccggcg gcggcgggcg tggtgctgga cgattcactg tcgccgcctc
    22801 cacaggcagc gagggcgagc atgcccgcca tcgcgatggc ggccagcttg gtgcgtcggg
    22861 tggtcattct tctccccctg agatctctgt cgggccgccg ttcggcccgc accgcaaccc
    22921 tagacggcgc ggcgggcagt ccggttcccg gcccggtttg tccgcgcgtc gcgtcactcc
    22981 ggccgcagga tcgcggcgag gtcgtagcgg gcgacttcct cgagctggtc gtaggtgcag
    23041 ctggccgggt cccggtccgg gcgccaccgc ttgaactggg cggtatgccg gaaccgtcgc
    23101 ccttccatgt ggtcgtagcc gacttcgagg acgcgctccg gacgcagcgg cacgaaggag
    23161 aggtccttgc cggcgctcca ccggctgcca gtgccgggca cgcggtcggg gttggcgatc
    23221 atccactgg
//
LOCUS       DAOPJG010000024        23162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2335_length_23162_cov_8.086381, whole genome shotgun sequence.
ACCESSION   DAOPJG010000024 DAOPJG010000000
VERSION     DAOPJG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 23162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23162
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2335_length_23162_cov_8.086381"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PKU75_04025"
     CDS             complement(<1..1163)
                     /locus_tag="PKU75_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPF80076.1"
                     /translation="MNERRRGLGKGLGALIPPAPTNGAGDGTDRPRPVDVFFPDGSPS
                     VDSRPESTVPQRDPARAGTVANRAGTVANRAGTVADSSGTVADSVGIVVDTAPAFAPP
                     ATSTTTPSDLVDVPGASFAEVAITDIRPNPRQPRSVFDEDELAELTASIREIGVLQPV
                     VVRPAQDGYELVMGERRWRAAGAAGLEQVPAIIRAVDDDDLLRDALLENLHRSQLNAL
                     EEAAAYQQLLDDFSCTQEELSARIGRSRPQISNTLRLLKLPPLVQRRVAAGVLSAGHA
                     RALLGLSDGGAMERLAQRVVAEGLSVRTVEEIVALGDIGNDEKPSHTRRPVAARPELV
                     EIESRLSDFLDTRAKIAMGAKKGRVTIEFATLEDLGRILSAMGVDPADRLPDDA"
     gene            complement(1160..2089)
                     /locus_tag="PKU75_04030"
     CDS             complement(1160..2089)
                     /locus_tag="PKU75_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF80077.1"
                     /translation="MVPIDVSRETEPERVSLFRVSREKPPVGDQQRSPKPATPRIVAV
                     SNQKGGVGKTTTAVNLAAALAQDGLGVLLIDMDPQGNASTALSIPHDEDDLGIYDVII
                     DGRPLIRVVKECPDITGLWCAPSTMDLAGAEIELVDMPHRERRLRQVIEQLYAALDRS
                     RRPAPDYVVIDCPPSLGLLTLNALVAADEVFIPIQCEYYALEGLTALMRTIGLVQGAL
                     NPTLTVGAILLTMVDGRTNLAAQVADEVRAHFPAQVLRNTIPRSVRLSESPSFGQTVM
                     TYDPTGTGALSYRAAAREYAHRQGGTHHKESAQ"
     gene            complement(2185..3582)
                     /gene="fumC"
                     /locus_tag="PKU75_04035"
     CDS             complement(2185..3582)
                     /gene="fumC"
                     /locus_tag="PKU75_04035"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPF80078.1"
                     /translation="MTRTEHDSMGAIEVPDDRYWGAQTERSLHHFDIGRDVFVWGRPM
                     IRALGVLKKAAAQANCELGELPAATGELIGRAADEVIAGTLDDHFPLVVFQTGSGTQS
                     NMNANEVIANRAAELAGGVLGSKQPVHPNDDVNRGQSSNDTFPTAMHIAVVSELRERL
                     YPGVTHLRETLAAKSAAYSDVVMVGRTHLQDATPVTLGQVIGGWVAQIDQAIAAVQRA
                     EDDARGLAIGGTAVGTGLNAHPDFGPRCAERISALTGIPFHQAPDLFAALSAHDAMLG
                     VSAALRTLAMALMKIANDVRWYASGPRNGLGELVIPENEPGSSIMPGKVNPTQCEALT
                     MVAAKVFGNDATVALADSQGNFQLNVFKPVIAWCVLESIRIIADACVSFTDHCAVGLE
                     PNRDRIAENLSTNLMLVTALNRHIGYDQAAAIAKRAHAAGLTLREAALESGAVTAEQF
                     DTWVVPADMTRPSGA"
     gene            complement(3639..3962)
                     /gene="trxA"
                     /locus_tag="PKU75_04040"
     CDS             complement(3639..3962)
                     /gene="trxA"
                     /locus_tag="PKU75_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPF80079.1"
                     /translation="MATLTTTDSTYELDVLQSDTPVLVDFWAPWCGPCRAVAPVLEEI
                     ARDNDGKIKVVKLNTDENPDVTRRYGITSIPTMHVVVGGEVVKTLVGAMPKPKLMREL
                     EPYLA"
     gene            complement(4010..4966)
                     /gene="trxB"
                     /locus_tag="PKU75_04045"
     CDS             complement(4010..4966)
                     /gene="trxB"
                     /locus_tag="PKU75_04045"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPF80080.1"
                     /translation="MITVSSEPTKAPVRSVIIIGSGPAGYTAAIYAARAALEPLVFEG
                     AVTAGGALMNTTEVENFPGFTDGIMGPDLMMNMRGQAERFGAEIITDDVAAVDLTGEI
                     KYVTDGEGNVHRARAVILAMGSAYRELGLPDEKRLSGHGVSWCATCDGFFFRDQDIVV
                     VGGGDSAIEEATFLTKFAKSVTIVHRRDELRASKIMADRAFADPKISFAWNSKVTAIH
                     GGDKLTGITLEDVNTGESRELPVTGLFIAIGHDPRNELVKGVVDLDDEGYVLAQERTS
                     LTNVAGVFACGDLVDKRYRQAITAAGSGCAAALDAEKFLSHN"
     gene            complement(5067..5909)
                     /locus_tag="PKU75_04050"
     CDS             complement(5067..5909)
                     /locus_tag="PKU75_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80081.1"
                     /translation="MNTPELGNVPIEDDPTGMRALLSSLPDPGPMPEELSARILAALE
                     REARYAEAGSIWDEEPPPEVPDVPERPANHPPAHGAHSTITPLVRKRPRWQPLLAAAA
                     AAGVLAFGGGAIFRLLNTGANSNNSAVGGAQNDASAKRENVEPAAPAATAHESSAAAS
                     ASSSAEVGGYAAGFRVLASGTAYSATDFAAQAGRVLSAPAPGSSTTPAGGLAVESAAR
                     ACAKAIGAPESSLLVVDFGTFDDSPAVLIVAEGTTGDPQAYVVTPECSATDDTVLAGP
                     AAVG"
     gene            complement(6001..6636)
                     /gene="sigM"
                     /locus_tag="PKU75_04055"
     CDS             complement(6001..6636)
                     /gene="sigM"
                     /locus_tag="PKU75_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HPF80082.1"
                     /translation="MSDIAGDATRPATPDGLLDLRECSDKGLMARHNDGDTDAFGELF
                     RRHKDRMWGVALRTCGNPDLAADAVQDAFISALRRSDSYRGDAAVTTWLHRITVNACL
                     DRLRRQKPTSELPEYDLTDGSDAPRDVDIRLTVQEALAKIPEQQRAALVLVDMYAVPV
                     AEAAEILGVAEGTVKSRCFRGRAALAEILADKAPETSGKSVENSMEPDGEA"
     gene            complement(6633..8741)
                     /locus_tag="PKU75_04060"
     CDS             complement(6633..8741)
                     /locus_tag="PKU75_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HPF80083.1"
                     /translation="MSGLQPGTVVGDQRYVTVRALFDHGDAEVWQAQDNSLEELVTLV
                     VVPMSSPTALAVLDAASRSAGLNNPRLVRVLDVGREYDYGYFVEESLDGAVTMTQLAA
                     GVGMEADEVRRMTGETALALDAAQRRGLHHLGLTPDNVVRMPDGSVKLAGLATQAALV
                     GQDHVGGKRALRRDAKGLVALAYAGLTGRWPFPGDVGLPPAPKVVSGVPRPSEIAVGV
                     PNDLDTICQLTLNQDLGPISPGDYAAQIAPWPDLQAPEQAALAIPAGSVEDYLAASPL
                     ASDAPAPDAAASDSAAAVTQALPVPAGDAVGQTRWLPSDDDAARRDAADARSAASDPD
                     AGAAAAEFGGEGGASATSAAVGGAGGSAAGMAAGASSAAGAGGAATGAGTAAGAAGAA
                     AGVGAVAGATAALSGALGSAGEAGRKLAGRLRQGASSMGAAARSATARAEGAVAEKRE
                     LKAAIRRVEADSEVTIDQTPQQPVLEPPVPLLPPESGAQPSRAQSRFVLASFAAATLA
                     AGLFAGAVAFRPPQSDLEAILGSDATAPVSTSTSSQSASPTSEDSAAPGTGEQLAIVG
                     ATGYDPEGDNLEYNDLAARVYDEDKASFWQSEGYQGPKFGGLKSGVGLIVDFGQDVTP
                     KQITLTLPNPSDLELYLGTSPDRAGANRIGTVSGEQGDVTLDVTDGAAGRYLIVWFTD
                     SPLVSDGRYRAMLAEVSATG"
     gene            complement(8812..10533)
                     /gene="murJ"
                     /locus_tag="PKU75_04065"
     CDS             complement(8812..10533)
                     /gene="murJ"
                     /locus_tag="PKU75_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPF80084.1"
                     /translation="MSQDAAHTPKAAQPPAPPATPPTGGGPESQRGQSLGRASALMAS
                     GTMVSRVLGMVRAALLVVVIGGTGLSADAFSVANTLPNQINLLVAGGMLGAVLVPQIV
                     KAAGRPDGGEDYVNRLLTLSLIVMAVTTAVGLAAAPLLVGLFSNAKDPAAIRLAVTFA
                     YLCLPQIFFYGLYTLLGNVLNARGRFTAFMWAPVLANVVSIIGLLIFTFSGYPRQAAP
                     GDWSSGMIWLLAGTMTLSIVVQAIFLVIPLRRSGFRYRPRFGFRGAGLRSASGVALWS
                     FAAVAVGQLGFIVTSRVLTRASDLAERDRIVAAGKASYDNAFLLFMLPHSLITVSLTT
                     ALFVRLATAAQHGNTRAVVADLNRGLRMPAPLTVPISLAGVLFAPLVVKLFFQQDQGT
                     TDAIAGVLVAMLLGVLPFGWLYLINRVYYAFEDGRTPFYFQVLVTAVATGFNLYAFTV
                     PVTQTGIWVGIGQTVSNAVGALLGFLALRRKLGLLGLSRTIRMHVRLVLASAVALALG
                     WLALAWIPSSWTDSVLGAAAVLLVLGPAYLVLAWGLGHLMRVREVDDLLAPVTRRLPR
                     VGTPRNE"
     gene            complement(10541..12646)
                     /locus_tag="PKU75_04070"
     CDS             complement(10541..12646)
                     /locus_tag="PKU75_04070"
                     /inference="COORDINATES: protein motif:HMM:NF039636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HPF80085.1"
                     /translation="MTSRRNPIRAFLRAPARVIGPALVVAGATAWVPAPATAASEAQI
                     VITINSLAPAVATGNEDVEVAGTVTNRGGADADIPEITLQGADTPLAGRAAVAAWASG
                     TTSGVPPASLRTYDTAAGPDRLSEGESHGFRLTLPKAAARTTAAYGALPLLVRAGDTV
                     RTTFAAVHNRKEYEQLRVAVVTPVTLDPEPGLFESYGAGRLASWRTELADDGRINRLL
                     DATKDLPVTWAFDPTLLAPPEAIAQERPTDPEAGTAWDRIGPEQREETALRIAFDAAL
                     RDRLPGRSVAVLPYADPDLAAVLADPGLTDRFAALRAKGEAVADGIPGARSDIAWPAD
                     GLLATSRVAAITELYGEGRLGAVIGSEAALGADNTLAAPHPGGADRAGAGVAALAYDD
                     GLSNALLDLESDPSGAITTQRLLADSLAALAEYPGTPRSLLIATPRTLDARPGALHRA
                     LSGLLDAPWITRGDLDDLIDEAPGGTPLPADPAAELALPSGIPADPSTERLALTASRA
                     RTVASALKAIDATSQVRDDGAVNGVFWDEVADQLLSSRWRADRGALRIVLGELRDAGI
                     QADTAVAVVPERINFFADSGLLQVTVTNDLDVPVRQVRVHLTPLAPSFRLTEMPEPVD
                     IGAHSRATVRVPATALAAGTVPVKVSLYSPQGQHIGVDNTVQVRAYPTGSWFYWVIGA
                     VAAALVAAGVLRSRRRRKA"
     gene            complement(12643..13119)
                     /locus_tag="PKU75_04075"
     CDS             complement(12643..13119)
                     /locus_tag="PKU75_04075"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPF80086.1"
                     /translation="MSQPVGRAPRRLPAVEERSAGGVVVDVQGGVARIAVIARRNRAG
                     RLEWCLPKGHIEGEETLSETAAREVAEETGIEARVLIKLGTIDYWFATGDRRVHKFVH
                     HYLLEAIGGFLTIENDPDQEAVDVAWLPLKDAHRHLTFPNERRIAREAWQRLAGSE"
     gene            13253..14713
                     /locus_tag="PKU75_04080"
     CDS             13253..14713
                     /locus_tag="PKU75_04080"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPF80087.1"
                     /translation="MDNPSPDAMRRAVAHLGPVLPLLSELGERFAAAGHELALVGGPV
                     RDAFLGRTSPDLDFTTSARPEEIERILGPWADTTWDIGKEFGTIGAVKGDATVEVTTY
                     RADAYDGVTRKPVVAFGDSLEDDLRRRDFTVNAMALRLPQLTFVDPYDGLDALVKRRL
                     RTPASPEVSFGDDPLRMMRAARFAAQLGFDLAADVAEAMQEMAGTIAIVSAERVRDEL
                     SKTLLAPAPRRGLVMLVDSGVADHVLPELPALQLEIDEHHRHKDVYEHTLSVLDQAIG
                     LEGPPAAPADAVPGPDLILRLAALLHDIGKPATRRFESGGGVSFHHHEVVGAKMTRAR
                     LRALRYDKETVKAVTRLVELHLRFHGYGDGAWTDSAVRRYVTDAGDLLPRLHLLTRAD
                     CTTRNQRKARRLAQTYDELESRIEELLAAEELAAVRPELNGNEIAEILGIPPGPVLGR
                     AYKFLLAVRLDDGPIGPEAAAERLRAWWAEQPESQA"
     gene            14819..>16969
                     /locus_tag="PKU75_04085"
     CDS             14819..>16969
                     /locus_tag="PKU75_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HPF80088.1"
                     /translation="MRVMAMGIHRSSDTRVRTRRRVAVLAATTTAAVTTALAGTTALA
                     GTTALAGGAAGAGASGIPQSGDLRLPGDTGARGQLGQHKIYIVPAYWQGDTPAQVSNA
                     AVQEAISEANDYWTDATDGKIMVASGWNVPKWQKLNLTSQQAASCDRKAIEAGVRAVV
                     GAAGERDHLVIVLNNERACDFTDIEQVGLTSRGDGLSFVNGTFDTTVALRVIAHNGGV
                     PGAGSLNCTDDSTPVPLSGTCTTKDYNNPWDPASGHPYGNVGTPIADTLATLGVLSGA
                     DYPEVTPGGVRTLTLDPLTASSGRRGFFVDLGRYRYSVDYRVPTGLDSWIDDKTWTSA
                     TGPLADPGGGVTVHRQDLKADKADRTRLLLDFHPDGSDSDTARHPGLAEGESWTSPDG
                     LWALKVGSATSSQASVTISFPGLGPVERWAGADRYETSAKISRNTYGTGVDVAYIASG
                     LVYTDALSGAPVAGMNDGPVLLVSDDSIPDPIAEELNRLKPKKIVIFGGTATISPQVE
                     EDLGSYTTGTVARWSGADRFATSAAISRESYQTGVDTAYIASGQVFTDALSGAPVAGK
                     KDGPVLLVDTNRLPGEIATELQRLNPTHIVILGGTATISENVEGALRPYSVNAIERWW
                     GADRFSTSAQIVHNSYSSTGGTVFVASGRVFSDALSGAPAAGTLEDPILLVDTDSVPD
                     AVRAELTRLKPAKIIILGGSATISYEVQAELAPFI"
     assembly_gap    16970..17069
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17319..19553
                     /locus_tag="PKU75_04090"
     CDS             17319..19553
                     /locus_tag="PKU75_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HPF80089.1"
                     /translation="MRHTGPRSARFEQVNSRHTAAPGAATGRRRYATATALVAALAAA
                     GAGVANAAPTPTHSTPTHSTPAHAVRADATAPAIPKIVQYADPAASARGVGERGALGE
                     HRVLVQPVYWTGSEPGALDTTAVAEAIGSANTYYRTSTNSAMSVTLAQTRPWKQITLT
                     AEEASSCDTEAIERETRKVAPDTPGVRKHLDIVFPETSACKFGALFSRGLTEAGDGVA
                     FLNGQQQVAWNLIAYGIGSNSGLGMANSISCWTDAAHTTPVPLSDYCKAEPGGDPWDL
                     MGWWHYGKVGKISAANLRRMGVLSDADFPEVTPGSGQYTFIRPLSAYRGQRGFAITVG
                     DTRYTVEYRTPTDLDSWIDDATWTDPTGVVRTDPGGGVIVRMQDLASETPADTTVLDF
                     HPDGKDVPTDRHPGLEPGEKWTSPDEVVRLEVVSATAKGASIKVDFPSLEKVERWSGA
                     DRYAASAAMSAKSFDPGVAVAYIASGEVYPDALSGAPVAGKDRGPVLLVEDDRLPGSI
                     QAELRRLTPGRIVILGGPATVGTAVADKLEDYTSGGVSRLFGEDRFATSAAISRDAFD
                     PGVPTVYIASGRIYTDALSGAPVAGKTATPVLLVDTDAIPASIAAELTRLKPGRIIVM
                     GGTSTITAKVETALRRYTSGGVLRYSGADRFDTSAAIAHENYNPGLAVVYVASGRVFP
                     DALSGAPIAGMTRGPVLLVDTDAVPPAIDVELERLKPRRIVVLGGPATVSERVRAVLG
                     SYLP"
     gene            19654..20361
                     /locus_tag="PKU75_04095"
     CDS             19654..20361
                     /locus_tag="PKU75_04095"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPF80090.1"
                     /translation="MIEVGVASHPGHYREQNEDCALAAYPIFAVADGMGGHAAGEVAS
                     ALAISVLRGLEGARLGEVEPVISAVEQANERIWAAGSEPGQRGMGTTLAGCAALTDGA
                     WVVFNVGDSRVLHIHEGEIRQVTIDHSEVQDMVALGILAPEEAQVHPMRHIVTRSLGA
                     RPDVDVAHERLEVTSGDRLLVCSDGLTDEVSPDDIFWVLDTARDPQDAADALVQAALA
                     GGGHDNVTVVVIDIRDA"
     gene            20506..21531
                     /locus_tag="PKU75_04100"
     CDS             20506..21531
                     /locus_tag="PKU75_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HPF80091.1"
                     /translation="MSSAVVRRGIVALVASGGLVVAPLAASTAFGADSGRAEGVERIA
                     GADRYETSAEISRRSFDSGVGTAFIASGEIFTDALSGAPVAAQRKGPLLLVKAGEIPS
                     AIAAELTRLRPRRIVVLGGPATISTSVESQLGSYTSGGVDRWMGKDRYEASAQISQET
                     FDPGVSKVYLASGEVFPDALSGAPVAGMDGAPMLLTADDRLPSAIAEELDRLNPKTIY
                     VLGGPNSVSDEVSAGLTDYTDGFVERLWGADRYDASANIAKESFAPGVSVAYVAYGQV
                     FSDALSGAPVGGIRRGPMLLVDDTDIPDSTREALGYLQPKKIVVLGGTASVNTAVEEE
                     LAGYVSP"
     gene            21622..22635
                     /locus_tag="PKU75_04105"
     CDS             21622..22635
                     /locus_tag="PKU75_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HPF80092.1"
                     /translation="MKSTITRRAALAAATLTGALALSPVAGANAADGGVYRIAGADRY
                     EASANIAHQSFLPGLDVAYVASGEVFTDALTGAPVAGMERAPVLLTKAESLPESVEAE
                     LMNLKPKKIIIFGGRASVSGAVANKLKKIATTGEVERIAGPTRYSTSAKISLENYEAG
                     VNTTYIASGEVFPDALSGAPVAAKTNGPLLLVEHDAVPGVVKNELKRLQTQHIVILGG
                     PATVSEEVESFLGKFTVGGTVTRQYGADRFDTSAAIAEANYAPGVETVYVASGRVFPD
                     ALSGAPVAGMKGSPVLLVDTDSIPASIDEQLKKLNPTNIVVLGGTATITKNVEDQLAA
                     YLS"
     gene            complement(22714..>23162)
                     /locus_tag="PKU75_04110"
     CDS             complement(22714..>23162)
                     /locus_tag="PKU75_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPF80093.1"
                     /translation="LNVGGNMDFKNMLERERLESKKISKTQAVTSNLNGPLAGKKEDR
                     NVHIGPSDYVAWLDDRKWAYVRLEGRAFGDVPLNLEYKLEVWDSPNSAGIIIDAIRAA
                     KIAKDRGVGGPLLSAAAYLMKSPPEQREDTEGRARLEAFIAGTEPR"
BASE COUNT         3420 a         7997 c         8208 g         3437 t
ORIGIN      
        1 gcgtcgtcgg ggaggcggtc ggcggggtcg acgcccatgg cagagaggat gcggccgagg
       61 tcttccaggg tcgcgaactc gatcgtgacc cggcccttct tggctcccat ggcgatcttg
      121 gcgcgggtgt cgaggaagtc cgagagacgg gactcgatct ccacgagctc gggccgagcg
      181 gcgacgggtc tccttgtgtg agagggcttt tcgtcattcc cgatatctcc gagagcgacg
      241 atctcctcca ccgtgcgcac cgacagtccc tctgccacga cgcgttgggc gaggcgctcc
      301 atggcgcctc catcggagag gccgaggagg gcgcgcgcgt ggccggccga caagacaccg
      361 gcggctaccc gtcgctgcac gagcggcggc agcttcagca gacggagcgt gttgctgatc
      421 tggggccggg agcggccgat gcgggcggag agttcctcct gcgtgcagga gaagtcgtcc
      481 agaagctgct ggtatgccgc ggcctcctcc agcgcattca gctggctgcg gtgcaaattc
      541 tccaggaggg catcgcgcag caggtcgtcg tcgtcgaccg cgcgaatgat tgcgggcacc
      601 tgttccaggc cggcggcgcc ggcggcccgc cagcgacgct cacccatgac gagttcgtag
      661 ccgtcctgtg cagggcgcac gaccaccggc tgcaggacgc cgatctcacg gatgctggcc
      721 gtcagttcgg ccagttcgtc ctcgtcgaac accgaacggg gctggcgcgg gttgggccgg
      781 atgtcagtga tcgcgacctc ggcgaagctt gcgccgggga cgtcgacgag gtcgcttggc
      841 gtcgtcgtcg aggtcgccgg cggggcgaag gcgggcgcgg tgtccaccac gattcccacg
      901 ctgtccgcca cagtgcccga gctgtccgct acagtacccg cgcggttcgc cacggtgcct
      961 gcgcggttcg ccacagtgcc cgcgcgtgcc ggatcgcgtt gcggcacagt ggattccggc
     1021 cgtgagtcga cggaaggcga gccgtcgggg aagaagacgt caaccggccg cggccgatcc
     1081 gtcccgtccc cggctccgtt ggtgggggcc ggcgggatga gggcgcccag gcccttgccg
     1141 agtccacggc gacgttcgtt cattgagcgc tctccttgtg atgggtgccg ccctggcgat
     1201 gggcgtattc gcgagccgcg gcgcgatagc tcaaggcgcc ggtgccggtc gggtcgtacg
     1261 tcatgacggt ctggccgaag ctcggtgact ctgacaggcg caccgagcgc gggatggtgt
     1321 tgcgcaggac ctgggccgga aagtgggcgc gcacctcgtc ggccacctgg gctgccaggt
     1381 tggtgcgccc gtccaccatc gtcagcagga tggcacccac cgtgagcgtg ggattcaagg
     1441 ccccctggac caatccgatc gtgcgcatca gcgccgtcag cccttccagg gcgtagtact
     1501 cgcactggat agggatgaag acctcatccg cggcaaccag cgcattgagc gtgaggagac
     1561 cgagactggg cggacagtcg atgaccacat agtcgggtgc ggggcgacga gaacggtcga
     1621 gggcggcata cagctgctcg atgacctgcc tcaaccggcg ctcccgatgc ggcatgtcca
     1681 cgagttcgat ctctgccccg gcgaggtcca tcgtcgaggg cgcgcaccac aatccggtga
     1741 tgtcggggca ctccttgacc acccggatca agggccggcc gtcgatgatg acgtcgtaga
     1801 tgccgaggtc gtcctcgtcg tgggggatgg acagcgccgt actggcatta ccttgcggat
     1861 ccatgtcgat gagcaggacg ccgagcccgt cctgggccaa ggcagcggcc aggttcacag
     1921 cggtcgtggt cttgccgacg ccgcccttct ggttggacac cgcgacgatc cgcggggtgg
     1981 cgggtttggg gctgcgctgc tggtctccga caggtggctt ttcacgtgaa acgcggaaga
     2041 gcgagacacg ttcgggctcg gtttcacgtg aaacatcgat cggcaccact ccaccctaac
     2101 cgccgatcgt cacgtgctcg tgcaccggcc gtgtcaggcg ccgctgcgtg tcagccgcat
     2161 gatggggttt caggctttgg gccctcaggc gcccgatggg cgagtcatgt ccgccgggac
     2221 cacccaggtg tcgaactgtt cggcggtgac agcacccgac tccagcgccg cctcgcgcag
     2281 agtcagcccg gcagcgtgcg cccgcttggc gatggcggcc gcctggtcat agccgatatg
     2341 ccggttcagc gccgtgacca gcatcaggtt cgtgctgagg ttctcggcga tgcggtcgcg
     2401 gttgggctcg aggccgacgg cgcagtggtc ggtgaacgag acgcacgcgt cggcgatgat
     2461 ccgtatcgac tcgagcacac accaggcgat gaccggcttg aagacattga gctggaagtt
     2521 gccctggctg tcggcgaggg caacggtggc atcgttgccg aataccttgg cagccaccat
     2581 cgtcagcgcc tcgcactggg tggggttgac cttgccgggc atgatggagc tgcccggctc
     2641 attctcggga ataactagct ctcccaaacc atttcgcggt cctgaggcgt accagcggac
     2701 gtcgttcgcg atcttcatca acgccatcgc cagcgtccgc agtgccgctg acacccccag
     2761 catggcgtcg tgggcggaca aggcggcgaa gaggtcgggt gcctggtgga agggtatgcc
     2821 ggtcagagcg gagatgcgtt cggcgcaccg agggccgaag tcgggatggg cgttgagtcc
     2881 cgtgcccacc gctgtgcctc cgatggccag tccacgtgcg tcgtcctcgg cccgctgcac
     2941 cgcggcgatc gcctggtcga tctgggccac ccagccgccg atgacctggc cgagggtgac
     3001 cggggtggcg tcctgcagat gggtgcggcc gaccatcacg acgtcgctgt aggcggcgga
     3061 cttggcggcg agcgtctcgc gcaggtgcgt gacaccggga tagaggcgct cgcgaagctc
     3121 gctgacgact gcgatgtgca tcgccgtggg gaaggtgtca ttggaggact gaccacggtt
     3181 gacgtcgtcg ttgggatgaa ccggctgttt cgatccgagg acaccgccgg ccagttcggc
     3241 agcccgattg gcgatgacct cgttcgcgtt catgttggac tgagtgcccg aaccggtctg
     3301 gaagaccacg agggggaagt ggtcgtccag agtgccggcg atgacctcgt cagccgcccg
     3361 cccgatcagc tcaccggtgg ctgcgggaag ctcaccgagc tcgcaattcg cctgtgctgc
     3421 agcctttttc aggacaccga gggctcggat catcggccgg ccccagacga agacatcccg
     3481 gccgatatcg aagtggtgga ggctgcgttc ggtctgggcg ccccaatagc gatcgtcggg
     3541 cacctcgatg gcgcccatgg agtcgtgttc ggtgcgagtc atggctccag tctcgcccgg
     3601 cctgcggccc gaaagcggca ggcgggaagc acggcaagct aggccaggta gggctcgagt
     3661 tcgcgcatga gcttgggctt gggcatggcg ccgacgaggg tcttgacgac ctcgccgccg
     3721 acgacgacgt gcatggtcgg gatggaggtg atgccgtagc ggcgggtgac gtcggggttc
     3781 tcgtcggtgt tcagcttgac gaccttgatc ttgccgtcgt tgtcgcgggc gatctcctcg
     3841 agcaccgggg cgacggcgcg gcaggggccg caccaggggg cccagaagtc gacgagcacg
     3901 ggggtgtcgc tctggaggac gtcgagttcg taggtggagt cggtggtggt cagggtcgcc
     3961 attgcggcat acctctcagt cggttattgg tggtctagaa gagacgaatt cagttgtggg
     4021 acaagaactt ttcggcatcg agtgccgcgg cgcagccgga gccggccgcg gtgatggcct
     4081 ggcggtaacg cttgtcgacc aggtcgccgc aggcgaagac gccggccaca ttggtcaggc
     4141 tggtgcgctc ctgggccaag acgtagccct cgtcgtcgag gtcgacgacg cccttgacga
     4201 gttcgttgcg cgggtcgtgc ccgatggcga tgaacaggcc ggtgacgggc agttcgcggg
     4261 actcgccggt gttgacgtcc tccagcgtga tgccggtcag cttgtcgccg ccgtggatgg
     4321 cggtgacctt gctgttccag gcgaaggaga tcttggggtc agcgaaggcg cgatcggcca
     4381 tgatcttgct ggcgcgcagc tcatctcgcc ggtggacgat ggtgaccgac ttggcgaact
     4441 tggtcaggaa ggtcgcctcc tcgatggccg agtcgccgcc gccgacgacg acgatgtcct
     4501 ggtcgcggaa gaagaagccg tcacaggtcg cgcaccacga gaccccgtgc ccggagaggc
     4561 gcttctcgtc gggcaggccg agctcacggt atgccgatcc catcgccagg atgaccgcgc
     4621 gggcccggtg cacattgccc tcgccgtcgg tgacgtactt gatctcgccg gtcaggtcga
     4681 ccgcggcgac gtcatcggtg atgatctcgg caccgaaccg ctcggcctgg ccgcgcatgt
     4741 tcatcatcag gtcgggcccc atgatgccgt cggtgaaccc ggggaagttc tcgacctccg
     4801 tggtgttcat cagcgcgccg ccggcggtga cggcaccctc gaagaccagc ggctccaagg
     4861 cggcgcgggc ggcatagatc gcggccgtat agccggcggg gccggagccg atgatgatga
     4921 cggagcgcac gggggccttg gtgggctcgc tcgacacggt gatcattcct tcgcggacgc
     4981 aagcaggtgg gtcgtctcgg gtcgccgccc gatgccgaag cgcgggcgat gcggtcttga
     5041 acagatccta ggcgctgctt gttccctcag ccgacggcgg ccgggccggc gagcacagtg
     5101 tcgtcggtcg ccgagcattc aggggtgacg acatacgcct gcgggtcgcc cgtggtgccc
     5161 tcggcgacga tcagcacggc cggggagtcg tcgaaggtgc cgaagtcgac gacgagcagg
     5221 ctggattcgg gtgctccgat cgctttcgcg cacgcgcggg ccgcggattc gacggcgagc
     5281 ccgccggcgg gggtcgtcga gctgcccggt gcgggtgcgg acaggacgcg acccgcttgc
     5341 gcggcgaagt ccgtggcgct gtatgccgtg ccgctggcca gaacgcggaa gcccgccgca
     5401 tagccgccga cctcggcgct cgaggaggcc gacgccgcgg ccgagctctc gtgtgcggtg
     5461 gccgccggtg ccgcgggttc cacgttctcc ctcttggccg aggcatcgtt ttgcgcgcca
     5521 ccgacagccg agttgttgct gttggccccg gtgttgagga gccggaagat cgccccacct
     5581 ccgaaggcga ggacgccggc ggccgcggcc gcggcgagca gtggttgcca cctcgggcgc
     5641 ttgcggacca gcggcgtgat cgtcgagtgg gcgccgtgag cgggcggatg gttcgccggg
     5701 cgctcgggca cgtcggggac ctcgggcggc ggctcctcgt cccagatgct gccggcctcg
     5761 gcgtatcgcg cctctcgctc cagggcggcc aggatgcggg cggacaattc ctcgggcatc
     5821 ggcccgggat cgggcaggga ggacagcaga gcgcgcatgc ccgtggggtc gtcctcgatg
     5881 gggacgttgc ccagctcggg ggtgttcacc gcctgctccc tcggtcgatc gaatgcgcgc
     5941 ggaggcggcc cgggcggggt ctccggtcgc gatacgcggg attggtaccc ggttgtgacg
     6001 tcatgcctcg ccgtccggtt ccatcgaatt ttcaacggat tttccggatg tctccggcgc
     6061 cttgtcggcg aggatctcgg ccagggccgc ccggccgcgg aagcagcggg acttgaccgt
     6121 cccctcggcg acgccgagga tctcggccgc ctccgcgacg ggcacggcat acatgtcgac
     6181 gagcaccaag gcggcgcgct gctgctcggg gatcttggcc agcgcctcct ggaccgtcag
     6241 cctgatgtcg acatcacgtg gcgcgtcgct gccgtcggtg aggtcgtatt cgggcaactc
     6301 gctggtcggc ttctgccggc ggagccggtc gaggcaggcg ttgacggtga tccggtgcag
     6361 ccacgtcgtg acggccgcat cgccgcggta gctgtcgctg cggcggagcg cggagatgaa
     6421 ggcgtcctgg acggcatcgg cggccaggtc ggggttgccg caggtgcgca gcgccacccc
     6481 ccacatgcgg tccttgtgcc ggcggaagag ttcgccgaac gcgtcggtgt cgccgtcgtt
     6541 gtgccgcgcc atgagcccct tgtcggagca ttcgcgcagg tcaagcagcc cgtccggggt
     6601 cgccggccgg gtggcatccc ctgcaatgtc gctcatccgg tcgcactgac ctcggcgagc
     6661 atggcgcgat agcggccgtc ggacacgagc ggtgagtcgg tgaaccacac gatgagatag
     6721 cggccggccg cgccgtcggt gacatcgagg gtgacatcgc cctgctcgcc actgaccgtc
     6781 ccgatgcggt tggctccggc gcggtcgggc gaggtgccga gatagagctc gaggtcggag
     6841 gggttcggca acgtcagggt gatctgcttg ggcgtgacat cctgaccgaa gtcgacgatg
     6901 agcccgacac cgctcttgag gccgccgaac ttcggtcctt gatagccctc ggactgccag
     6961 aaactcgcct tgtcctcgtc gtagacgcgc gcggccaggt cgttgtattc gaggttgtcg
     7021 ccctccgggt cgtatccggt ggcgccgacg atggcgagct gctcgcccgt gccgggggcg
     7081 gcggagtcct cgctggtcgg gctggcactc tggctgctgg tagacgtgct cacgggcgcc
     7141 gtcgcgtcgg atccgaggat ggcctcgagg tcggactgcg gcggccggaa cgcgactgcc
     7201 ccggcgaaga ggccggcggc aagggtcgcc gcggcgaagg acgcgaggac gaagcggctc
     7261 tgggcgcggc tcggctgcgc accggactcg ggcgggagca gcgggaccgg gggttcgagg
     7321 acaggctgct gcggcgtctg gtcgatcgtg acctcggagt cggcctcgac gcgccggatc
     7381 gcggccttga gctcacgctt ctcggccacc gcaccctcgg ctcgcgcggt ggccgaccgg
     7441 gccgcagccc ccatcgacga tgcgccctgg cgcagccttc cggcgagttt tcgcccggcc
     7501 tcgcccgccg aacccagcgc gccggacagg gcggcggtgg cccccgccac ggcgcctacg
     7561 cctgctgcgg cgccggccgc accagctgcg gtgcctgccc cagttgcggc cccgcccgca
     7621 ccggctgcgg aactcgcgcc tgctgccata ccggcagcac tgcctccggc accgccgaca
     7681 gcagcagagg tagccgatgc gccgccttcg ccgccgaact cggcggcggc cgcgcccgcg
     7741 tcgggatcgg aggcggccga ccgggcgtcg gccgcgtcgc ggcgggccgc atcgtcatcg
     7801 ctcggcaacc accgggtctg gccgaccgcg tcgccggccg ggaccggcag ggcctgcgtg
     7861 accgccgctg cggagtcgga ggcggcggca tcgggcgcgg gcgcatcgga cgcgagcggc
     7921 gacgccgcga ggtagtcctc gacggaaccg gccgggatgg ccagggccgc ctgctcgggc
     7981 gcctgcaggt ccggccacgg ggcgatctgc gcggcatagt cgcccggcga gatcgggccg
     8041 aggtcctggt tcagggtgag ctggcagatg gtgtccaggt cgttggggac gccgaccgcg
     8101 atctccgacg ggcgcggcac gcccgagacg accttgggcg cgggcgggag acccacgtcc
     8161 cccgggaacg gccagcgccc ggtcagcccg gcatacgcca gcgcgaccag gcccttggcg
     8221 tcgcgccgca acgcgcgctt gccgccgacg tgatcctggc ccacgagggc cgcctgcgtg
     8281 gccagcccgg cgagcttgac cgagccgtcc ggcatccgca cgacattgtc cggcgtcagg
     8341 ccgaggtgat gcaggccgcg ccgctgcgcg gcgtcgaggg ccagcgctgt ctcgccggtc
     8401 atccggcgca cctcgtcggc ctccatgccg accccggcgg ccagctgcgt catcgtcacg
     8461 gcgccgtcga gcgactcctc gacgaaatag ccgtagtcgt attcgcggcc cacgtccagg
     8521 acccgcacca ggcgcgggtt gttcaggccc gcgctgcggc tcgcggcatc gagcacggcc
     8581 agcgccgtcg ggctggacat cggcacgacg acgagggtga ccagctcctc cagggagttg
     8641 tcctgggcct gccagacctc ggcgtcgcca tggtcgaaca gcgcacgcac ggtgacatat
     8701 cgctgatcac cgacgacggt tccgggttgc aggcccgaca cattggctcc ttctgctgct
     8761 cggccatgga cgcgatcgac ccgccacagg ctggcccatc ctaattgtcc gctactcgtt
     8821 gcggggcgtg ccaacgcgcg gcaaccggcg ggtcaccggc gcgagcaggt cgtccacctc
     8881 gcgcacccgc atcagatgcc ccagacccca cgcgaggacg agataggccg gtcccagcac
     8941 gagcaggaca gccgccgccc cgagcaccga gtcggtccac gaggagggga tccacgccag
     9001 cgcgagccac cccagcgcga gggccacggc gctggcgagg acgaggcgca catgcatacg
     9061 aatggtcctg gacaggccga gcagcccgag cttgcgccgc agcgcgagaa aacccagcag
     9121 cgcccccacg gcgttgctca cggtctgtcc gatgccgacc cagatgccgg tctgcgtcac
     9181 cggcaccgtg aacgcgtaga ggttgaagcc ggtcgccacc gccgtgacga gcacctggaa
     9241 gtagaacggc gtgcgcccgt cctcgaaggc gtaatagacc cggttgatca gatagagcca
     9301 gccgaacggc aacaccccga gcagcatggc gacgagcact cccgcgatgg catcggtcgt
     9361 gccctgatcc tgctggaaga agagcttgac gaccaggggt gcgaagagca ccccggccag
     9421 ggagatcggg accgtcaggg gggccggcat ccgcaggccg cggttcaggt cggcgacgac
     9481 cgcgcgggtg ttgccatgct gggccgccgt ggcgagccgc acgaagagcg ccgtcgtgag
     9541 cgagacggtg atcagggagt gcgggagcat gaacagcagg aaggcgttgt cgtatgacgc
     9601 cttgccggcc gcgacgatcc ggtcccgctc cgccagatcg ctggcgcgcg tcaggacgcg
     9661 gctggtgacg atgaagccga gctggccgac cgcgacggcg gcgaaggacc acagcgccac
     9721 acccgaggcc gaccgcaagc cggcgccgcg gaaaccgaac cgggggcgat agcgaaaacc
     9781 gctgcgccgc aacggaatca ccaggaagat cgcctgcacc acgatcgaga gcgtcatcgt
     9841 cccggcgagg agccagatca tccccgacga ccagtcgccc ggtgccgcct gccgcggata
     9901 gccggagaag gtgaagatca gcagaccgat gatcgagacc acattggcca gcaccggcgc
     9961 ccacatgaac gccgtgaacc ggccgcgcgc gttgaggaca ttgccgagca gcgtgtagag
    10021 cccgtagaag aagatctgcg gcagacacag ataggcgaag gtcaccgcga gccggatcgc
    10081 ggccgggtcc ttggcgttgc tgaacaggcc cacgagcagc ggcgccgccg ccagcccgac
    10141 agcggtcgtg acggccatga cgatgaggga gagcgtcagg agccggttga catagtcctc
    10201 gccgccgtcg ggccggccgg ccgccttgac gatctgcggc accaggaccg ccccgagcat
    10261 gccccccgcg acgagcaggt tgatctggtt gggcagggtg ttcgccaccg agaaggcgtc
    10321 ggcggacagc ccggtgccgc cgatgacgac gacgagcagc gccgcccgca ccatgccgag
    10381 cacgcgcgag accatcgtcc cggacgccat cagcgcgctg gcgcggccca ggctctggcc
    10441 gcgctggctt tcggggccgc ccccggtggg cggggtggcc ggcggcgcag gcggctgcgc
    10501 cgctttgggg gtatgagcag cgtcctggct cacgagtagg tcaggccttc cgacggcggc
    10561 gggagcgcag cacaccggcg gcgacgagtg ccgcggcgac cgcgccgatc acccaataga
    10621 accaggagcc ggtgggatag gcgcgcacct ggacggtgtt gtcgacgccg atgtgctgac
    10681 cttgtgggct gtacaggctc accttgacgg gcacggtgcc cgcggccagc gcggtggccg
    10741 gcacccgcac ggtggcccgc gagtgcgccc cgatgtcgac cggctcgggc atctcggtga
    10801 gccggaagct cggcgcgagc ggcgtcagat gcacccggac ctgccgcacc ggcacgtcga
    10861 ggtcgttggt gaccgtcacc tggagcaggc ccgagtcggc gaagaagttg atgcgctcgg
    10921 gcacgaccgc gacggcggtg tcggcctgga tgcccgcgtc gcggagttcg ccgagcacga
    10981 tccgcaaggc gccccggtcg gcccgccacc ggctggagag cagctggtcg gcgacttcgt
    11041 cccagaagac gccgttgacg gccccgtcgt cacgaacctg cgaggtcgcg tcgatcgcct
    11101 tgagggcact ggccaccgtg cgggcccggc ttgccgtcag cgccagccgt tcggtgctcg
    11161 ggtcggccgg gatcccgctc gggagcgcga gttccgccgc cggatccgcg ggcagcgggg
    11221 tgccgcccgg ggcctcgtcg atcaggtcat cgaggtcgcc gcgggtgatc cacggcgcgt
    11281 cgagtaggcc ggacagtgcc cggtgcaggg cgcccgggcg ggcgtcgagc gtgcgcgggg
    11341 tcgcgatcag caggctccgc ggggtgccgg ggtattccgc cagcgcggcg agcgagtccg
    11401 cgagcagccg ttgcgtggtg attgccccgg aggggtcgct ctcgaggtcg aggagggcat
    11461 tgctcagacc gtcgtcatag gccagcgcgg ccacacccgc gccggcccgg tccgcgccac
    11521 ccgggtgcgg ggcggcgaga gtgttgtccg cgccgagggc ggcctccgag ccgatgacgg
    11581 cacccagccg gccctcgccg taaagctcgg tgatcgccgc gacccgcgat gtcgcgagca
    11641 gcccgtcggc cggccacgcg atatccgatc gcgcccccgg gatcccgtcc gcgaccgcct
    11701 cccccttcgc ccgcagggcg gcgaaccggt cggtaagccc cggatcggcg agcaccgcgg
    11761 cgaggtccgg atcggcatac ggcagcacgg cgacggaccg gcccggcaac cggtcgcgca
    11821 gcgccgcgtc gaaggcgatg cgcagcgcgg tctcctctcg ctgctccgga ccgatccggt
    11881 cccacgcggt gccggcctcg ggatcggtcg gccgctcctg cgcgatcgcc tcgggcggcg
    11941 ccaggagtgt cgggtcgaac gcccaggtca ccggcagatc cttggtggcg tcgaggaggc
    12001 ggttgatgcg gccgtcgtcg gcgagttcgg tgcgccacga cgcgagccgg cccgcgccat
    12061 acgactcgaa caggcccggc tccgggtcga gggtgaccgg agtgaccacg gcgacccgca
    12121 gctgctcgta ctccttgcgg ttgtgcaccg cggcgaacgt ggtgcgcacc gtatcgcccg
    12181 cgcgcaccaa cagcggcagg gcgccgtatg ccgcggtcgt ccgggcggcc gccttgggca
    12241 gcgtcagccg gaagccgtgg ctctcgccct cggagaggcg gtcggggccg gcggccgtgt
    12301 cataggtccg cagggacgcc gggggaaccc cgctcgtcgt gccgctcgcc caggcggcaa
    12361 ccgcggcgcg gccggcgagc ggggtgtcgg cgccctggag ggtgatttcg ggtatgtcgg
    12421 cgtccgcgcc cccgcggttg gtgacggtgc cggcgacctc gacgtcctcg ttcccggtcg
    12481 cgacggccgg ggcgagggag ttgatggtga tgacgatctg agcctcggac gccgcggtgg
    12541 cgggggccgg cacccacgcc gtcgcgccgg ccacgacgag tgccggtcca atgacccggg
    12601 ctggtgcgcg gaggaaggcg cggatcgggt tgcggcggct ggtcattcgc tcccggcaag
    12661 gcgctgccat gcctcccggg cgatgcgccg ttcgttgggg aaggtcaggt gccggtgggc
    12721 gtccttcagc ggcagccacg cgacatcgac ggcctcttgg tcggggtcgt tctcgatggt
    12781 gagaaaaccg ccgatggcct ccagcagata gtggtggacg aacttgtgca cgcggcggtc
    12841 gcccgtggcg aaccaatagt cgatcgtgcc gagcttgatc agcacgcgtg cctcgatgcc
    12901 ggtctcctcg gcgacctccc gggccgccgt ctccgacagc gtctcctcgc cctcgatgtg
    12961 gcccttgggc aggcaccact cgagacgtcc ggcgcggttg cggcgggcga tgacggcgat
    13021 gcgcgccacc cctccctgga cgtcgacgac gacaccgccg gccgaccgct cctccacggc
    13081 gggcaaccgc cgcggcgcgc gtccgacggg ctgactcatg gggtcactct aaaggggcgt
    13141 cctggccgga tgcccgaagt tcgatgcgcg acaccgggac ccgcaaaggt ggcaccacgc
    13201 ccgcggtggg cggttcgtgc ggaggtgggc acgcagcata cggttggacg tcatggacaa
    13261 ccccagcccc gacgcgatgc ggcgtgccgt cgcgcacctc gggccggtgc tgcccctgtt
    13321 gagcgagctc ggcgagcgtt tcgcggccgc cggccacgaa ctggcactcg tcggcgggcc
    13381 ggtgcgcgac gccttcctcg gccgcacctc ccccgacctg gacttcacga ccagcgcgcg
    13441 gcccgaggag atcgaacgga tcctggggcc gtgggccgac acgacgtggg acatcggcaa
    13501 ggagttcggc acgatcgggg cggtcaaggg ggacgcgacg gtagaggtga cgacctatcg
    13561 cgccgacgcc tacgacgggg tgacacgcaa gccggtggtc gcgttcggcg actcgctcga
    13621 ggacgatctg cggcggcgcg acttcaccgt caacgcgatg gcgttgcggc tgccacagct
    13681 gaccttcgtc gatccgtatg acggtcttga cgccctcgtg aaacggcgcc tgcgcacccc
    13741 ggcctcgccc gaggtgtcct tcggcgacga cccgctgcgg atgatgcggg cggcccggtt
    13801 cgcggcgcag ctggggttcg acctggcggc cgatgtcgcc gaggcgatgc aggagatggc
    13861 cggcacgatc gcgatcgtct cggccgagcg ggtgcgcgac gaactctcca agaccctcct
    13921 ggcaccggcg ccgcggcgcg ggctggtcat gctcgtcgac agcggtgtgg ccgaccatgt
    13981 cctgccggag ttgccggcgc tgcagctcga gatcgacgaa caccaccggc acaaggacgt
    14041 ctacgagcac acgctcagcg tgctcgacca ggccatcggg ctggaggggc cccccgcggc
    14101 tcccgcggac gcggtgcccg gacccgacct gatcctgcgg ctcgcggcgc tgctgcacga
    14161 catcggcaag cccgcgaccc ggcgcttcga gagcggcggc ggggtctcct tccaccacca
    14221 cgaggtggtc ggggccaaga tgacccgggc gcggctgcgg gctttgcgct acgacaagga
    14281 gacggtcaag gcggtcaccc gcctcgtcga actccacctg cgcttccacg gctacggcga
    14341 cggggcgtgg accgactcgg ccgtgcgtcg ctatgtcacc gacgccggcg acctgttgcc
    14401 ccggctgcac ctgttgaccc gcgccgactg cacgacccgc aaccagcgca aggcgcgccg
    14461 gctcgcgcag acctatgacg agctcgagtc ccggatcgag gagttgctcg ccgccgagga
    14521 gctggcggcc gtgcgtccgg aactcaacgg caacgagatc gccgagatcc tcgggatccc
    14581 accgggaccg gtcctgggcc gggcctacaa gttcctgctc gcggtgcgcc tggacgacgg
    14641 ccccatcggg cccgaggccg cggccgagcg gttgcgcgcc tggtgggccg agcagcccga
    14701 aagccaggcg tgaggcaccc acgcagccgg tcctcccatc cggcgtcgtg gttctcgatt
    14761 cggcaacggc ccggcgcgga ccagccgctt tgtcgtttgc agccggtagc gtcagcgcat
    14821 gcgcgttatg gctatgggga ttcaccgcag cagcgacacg cgagtgcgca cccggcgacg
    14881 ggtggctgtc ctggcggcca cgaccacggc agcggtgacc acggcgctgg ctgggacgac
    14941 ggcgctcgcc gggaccacgg cgctggcggg cggggcagcg ggggcgggag cgagcggcat
    15001 accgcaaagc ggtgacctgc gactgcccgg cgacaccggc gcacgcggcc agctcggcca
    15061 gcacaagatc tatatcgtgc cggcctactg gcagggcgac accccggcac aggtgtcgaa
    15121 tgccgctgtg caggaagcca tttcggaggc caacgactac tggaccgatg cgaccgacgg
    15181 caagatcatg gtcgcgtccg gctggaatgt gcccaagtgg cagaagctca acctcacgtc
    15241 ccagcaggcc gccagctgcg accggaaagc catcgaggcc ggtgtgcggg cggtggtcgg
    15301 ggccgcgggc gagcgcgacc acctcgtcat cgtgttgaac aacgagcgcg cgtgcgattt
    15361 caccgatatc gagcaggtcg gcctcaccag ccggggcgac ggtctctcgt tcgtcaacgg
    15421 cactttcgac acgaccgtgg cgctgcgggt catcgcgcac aacggcggcg tgccgggcgc
    15481 gggctcgctc aactgcaccg acgactccac gcccgtgccg ctgtcgggca catgcacgac
    15541 caaggactac aacaacccgt gggatccggc gagcggtcat ccctacggaa acgtcggcac
    15601 cccgatcgcc gacaccctcg ccacgctcgg ggtgctctcc ggcgcggact accccgaagt
    15661 cacccccggc ggcgtgcgca ccttgaccct cgacccgctc acggcgagca gtggccggcg
    15721 tggtttcttc gtggacctgg gtcgctaccg ctactcggtc gactatcgcg tgccgaccgg
    15781 actcgacagc tggatcgacg acaagacctg gaccagcgcg accgggccat tggccgaccc
    15841 gggtggcggg gtgaccgtcc accggcagga cctcaaggcc gacaaggcgg accggacgcg
    15901 gttgctcctc gacttccacc cggacggctc cgactccgac acggcccgcc atcccggtct
    15961 cgcggagggc gagagttgga ccagccccga cgggctgtgg gcgctgaagg tgggctcggc
    16021 cacgtcgagc caggcgagcg tgacgatctc cttccccggt ctgggcccgg tcgaacgctg
    16081 ggcgggtgcg gaccgctacg agacgtccgc caagatctcg cgaaacacct acggcaccgg
    16141 cgtcgatgtc gcctatatcg cctcggggct ggtctacacc gacgcgctgt cgggcgcccc
    16201 ggtcgccggg atgaatgacg gcccggtgct gctcgtcagc gacgactcca tccccgaccc
    16261 catcgccgag gagctgaacc ggctcaagcc gaagaagatc gtcatcttcg gtggcaccgc
    16321 gaccatctcg ccacaagtgg aggaggacct cgggagctac accacgggca ccgtcgcgcg
    16381 ttggtcgggt gcggaccgct tcgccacctc ggccgccatc tcccgggaga gttaccagac
    16441 cggcgtcgac accgcctata tcgcctccgg ccaggtcttc accgacgccc tgtccggggc
    16501 accggtcgcc ggcaagaagg acggcccggt gctgctcgtc gacaccaacc ggctgcccgg
    16561 agagatcgcc accgaactgc agcggctcaa cccgacacac atcgtcatcc tcgggggcac
    16621 cgcgacgatc agcgagaacg tcgagggcgc cctgcggccc tactcggtca acgccataga
    16681 acgctggtgg ggtgcggacc ggttctcgac ctccgcccag atcgtccaca actcctacag
    16741 cagcaccggc gggaccgtct tcgtcgcgtc cggtcgcgtc ttctccgatg ccctctccgg
    16801 cgcgcctgct gccggcacgc tcgaggaccc gatcctgctc gtcgacaccg actccgttcc
    16861 cgacgccgtc cgcgccgaac tcactcggct caagcccgcc aagatcatca tcctcggcgg
    16921 ctcggcgacg atctcctacg aggtgcaggc cgagctggcg cccttcatcn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggcggctcgg cgacgatctc ctacgaggtg
    17101 caggccgagc tggcgccctt catccactag gccacccacg aagggtccgc gctccttcgt
    17161 cgctccggta cttcgcgggg accccgccct gtcggtggat cgtcgtcata cgtcctagcc
    17221 ctggcatagg tcggacgcac acggtttccc ggtctcgatc cggcaagtcg ccagcggaat
    17281 ccatgacacc cgtggcaccc acgggtaccg tgagaaacat gcgtcacacg gggcccaggt
    17341 cggccaggtt cgagcaggtc aactcacgtc acacggctgc gccgggcgcg gccaccggcc
    17401 ggcgccgcta cgccacagcg actgcgctgg tcgcggccct cgcggcggcg ggcgcgggcg
    17461 tcgccaacgc ggcaccgaca ccaacgcact ccacgccaac gcactcgacg ccggcgcacg
    17521 cggtccgcgc cgacgcgacc gcaccggcca tacccaagat cgtgcagtat gccgaccctg
    17581 cggcgtccgc tcgcggcgtc ggcgagcgcg gcgcgctggg tgagcatcga gtgctggtgc
    17641 agccggtcta ctggaccggg tccgagccgg gggcgctcga caccacggcg gtggcggagg
    17701 ccatcgggtc ggccaacacc tactaccgca cctccaccaa ctcggccatg tcggtcaccc
    17761 tcgcgcagac ccggccgtgg aagcagatca ccctcaccgc cgaggaggcc tccagctgcg
    17821 acaccgaggc catcgagcgc gagacccgca aggtggcgcc cgacaccccg ggcgtgcgca
    17881 aacacctcga catcgtcttc ccggagacct ccgcttgcaa gttcggtgcc ctcttctcgc
    17941 gcggcttgac cgaagccggt gatggtgtcg ccttcctcaa cggccagcag caggtcgcct
    18001 ggaacctgat cgcctacggg atcggcagca actccggtct cggtatggcg aattcgatca
    18061 gctgctggac cgatgcggcg cacaccactc ccgtcccgct gtcggactac tgcaaggccg
    18121 aacccggcgg cgacccctgg gacctgatgg gctggtggca ctacggcaag gtcggcaaga
    18181 tctccgccgc caacctgcgc cggatggggg tgctcagcga cgccgacttc cccgaggtga
    18241 cgcccggcag cgggcagtac accttcatcc ggccgctgtc ggcctaccgg ggccagcgcg
    18301 gcttcgcgat cacggtcggc gacacccgct acaccgtcga ataccgcacg cccaccgacc
    18361 tcgactcgtg gatcgacgac gcgacgtgga ccgatccgac gggcgtcgtg cgcaccgatc
    18421 ccggtggcgg cgtcatcgtg cgcatgcagg acctggccag cgagacgccc gccgacacga
    18481 ccgtcctcga cttccacccg gacggcaagg acgtccccac cgaccggcac cccggcctag
    18541 agcccggtga gaaatggacc agtcccgacg aggtcgtcag gctcgaggtc gtctcggcca
    18601 ccgccaaggg cgcgagcatc aaggtcgact tcccgtcgct cgagaaggtg gagcggtggt
    18661 ccggcgccga ccggtatgcc gcgtccgccg ccatgtcggc caagagcttc gaccccgggg
    18721 tcgcggtcgc ctatatcgcc tccggtgagg tctaccccga cgccctctcg ggcgcgccgg
    18781 tcgccggcaa ggacagggga ccggttctgc tcgtcgagga cgaccgctta ccgggaagca
    18841 ttcaggcgga gctgcggcgc ctgacgcccg ggcgcatcgt catcctcggc ggcccggcca
    18901 ccgtcggcac ggcggtggcg gacaagctcg aggactacac ctccggcggc gtgagccggc
    18961 tcttcggcga ggaccggttc gcgacctcgg ccgcgatctc ccgggatgcc ttcgaccccg
    19021 gcgtgccgac cgtctacatc gcctcgggcc ggatctacac cgacgccctc tcgggtgcgc
    19081 cggtcgccgg caagaccgcc acgccggtcc tgctcgtcga caccgacgcg atccccgcca
    19141 gcatcgccgc cgaactgacc cggctcaagc cgggccgcat catcgtgatg ggcggcacca
    19201 gcaccatcac cgcgaaggtc gagaccgcac tgcggcgcta caccagcggg ggggtgctgc
    19261 gctactccgg cgccgatcgc ttcgacacct ccgcggccat cgcgcacgag aactacaacc
    19321 ccggcctcgc cgtcgtctac gtcgcgtcgg gccgggtctt ccccgacgcc ctgtcgggag
    19381 ccccgatcgc cggtatgacg cgtggtcccg tcctgctggt cgacaccgat gcggtgccgc
    19441 cggcgatcga cgtggaattg gagcgcctga agccgcgccg gatcgtcgtc ctcggcggcc
    19501 cggcgacggt cagcgagcgg gtgcgcgcgg tcctcgggtc ctacctgccc tgaccaggat
    19561 ctaccctcct cacggcgcca cgacagacgt ggcgccgtga ggtattttgg gtgggcttgc
    19621 caggtggtcg gtccggtgtg ggacattagc gccgtgatcg aggtcggcgt ggcgtcccac
    19681 ccggggcatt accgggagca gaacgaggat tgtgccctcg ctgcctaccc cattttcgct
    19741 gtcgccgacg ggatgggcgg acatgccgcc ggcgaggtcg cgagcgcgct cgccatctcg
    19801 gtcctgcgcg gtctggaggg cgcccggctg ggcgaggtcg agccggtgat ctcggccgtg
    19861 gagcaggcca acgagcggat ctgggcggcc gggtcggagc cgggtcagcg cggcatgggc
    19921 accacgctcg ccggctgcgc ggcgctcacc gacggcgcct gggtggtctt caacgtcggc
    19981 gactcgcggg tgctgcacat ccacgagggc gagatccggc aggtcacgat cgaccactcc
    20041 gaggttcagg acatggtggc cctcggcatc ctggcgcccg aggaggccca ggtgcacccg
    20101 atgcgccaca tcgtcacccg ctcgctcggc gcccggcccg atgtcgacgt tgcccacgag
    20161 cggctcgagg tcaccagcgg tgaccggttg ctcgtctgct ccgacggcct gaccgacgag
    20221 gtgagtccgg acgatatctt ctgggtcctc gacacggccc gggatcccca ggacgccgcc
    20281 gacgccctgg tgcaggccgc cctcgcaggt ggtgggcacg acaatgtcac cgtcgtcgtg
    20341 atcgacatcc gggacgcgtg atctgcgcct gatacgcatg cttcacgtgt gaaatgtccg
    20401 tcctccgggc atcaggtgtg tcctcacccg ggcgaattcg ttaccctcct ggggacgacc
    20461 gacccatttg ggtcgtcggg gcagtcgttt cgaggagatt cacacatgag cagtgcagtt
    20521 gtccggcggg gaatcgttgc gctcgtggcg tccggagggc tcgtcgtcgc tccgctagcc
    20581 gcgtccaccg ccttcggggc ggattctggc cgggccgaag gggtcgaacg cattgcgggc
    20641 gccgaccgct acgagacgtc ggcggagatc tcccgacgca gcttcgactc gggggtgggc
    20701 acggccttca tcgccagtgg tgagatcttc accgacgccc tgtcgggtgc accggtggcg
    20761 gcccagcgca agggccccct gctgctggtc aaggcgggcg agatcccctc ggccatcgcc
    20821 gccgagctga cccggctgcg gccgcggcgc atcgtggtcc tcggcggtcc ggccaccatc
    20881 agcacctcgg tggagtccca gctgggcagc tacaccagcg gcggggtgga ccgctggatg
    20941 gggaaggacc ggtatgaggc gtccgcccag atctcgcagg agaccttcga cccgggcgtg
    21001 tccaaggtct acctcgcgtc cggcgaggtc ttcccggacg ccctctccgg cgcccccgtc
    21061 gccggcatgg acggcgcgcc catgcttctg actgctgacg accggctgcc cagcgcgatc
    21121 gccgaggaac tcgaccggct caaccccaag accatctacg tcctcggcgg acccaacagc
    21181 gtttccgacg aggtctcggc cgggctgacg gactacacgg acggcttcgt cgagcggctc
    21241 tggggcgcgg accgctacga cgcctcggcg aacatcgcca aggagtcgtt cgcccctggc
    21301 gtctctgtcg cctatgtcgc ctacggccag gtcttctccg atgcgctgtc cggcgcgccg
    21361 gtcggcggca tccgccgcgg cccgatgctg ctcgtcgacg acaccgacat ccccgactcc
    21421 acccgcgagg cgctcggcta cctgcagccc aagaagatcg tcgtgctcgg tggcaccgct
    21481 tcggtcaaca ccgccgtcga ggaggaactg gccggctacg tctctccgtg aggccggtcc
    21541 ggcccggggg ccggctcggt ttcgccccgt cggctcgcgt catacggcgt gggtcacccc
    21601 gcaaaggaag tgaatcagtt catgaagagc accatcaccc ggcgcgccgc gctcgcggcc
    21661 gccacgctga ccggcgccct cgccctcagc cccgtcgccg gcgccaatgc cgccgacggc
    21721 ggcgtgtacc ggatcgccgg cgccgaccgc tacgaggcct ccgccaatat cgcccaccag
    21781 agcttcttgc cgggcctcga tgtcgcctat gtcgcctccg gtgaggtctt caccgacgcc
    21841 ctcaccggtg cgcccgtggc gggcatggag cgcgctccgg tgttgctgac caaggcggag
    21901 agcctgccgg agtcggtcga ggccgaactg atgaacctga agccgaagaa gatcatcatc
    21961 ttcggcggcc gcgcctccgt ctcgggcgcg gtggccaaca agctcaagaa gatcgccacg
    22021 accggcgagg tcgagcgcat tgccggcccc actcgctaca gcacgtcggc gaagatcagc
    22081 ctggagaact acgaggccgg cgtcaacacg acctatatcg cctccggcga ggtcttcccc
    22141 gacgcgctct ccggtgctcc ggtcgcggcc aagaccaacg gtccgctgct gctcgtcgag
    22201 cacgacgccg tcccgggcgt cgtcaagaac gaactgaagc gcctccagac ccagcacatc
    22261 gtcatcctgg gcggtcccgc gaccgtcagc gaggaagtcg agagcttcct cggcaagttc
    22321 accgtcggcg gcacggtcac ccgccagtac ggcgcggacc gcttcgacac ctctgcagcg
    22381 atcgcggagg ccaactacgc cccgggcgtc gagacggtct acgtcgcctc cggccgcgtc
    22441 ttccccgacg cgctctccgg cgccccggtc gccggcatga agggctcgcc ggtgctgctc
    22501 gtcgacaccg attcgatccc ggcgtcgatc gatgagcaac tgaagaagct caacccgacg
    22561 aatatcgtcg tcctcggcgg caccgccacc atcaccaaga acgtcgagga tcagctcgcc
    22621 gcatacctca gctgagttcg cttccgcgac aatgcaagcg ggcggcatac ccaaccctgg
    22681 gtatgccgcc cgctcgcgtt tgcgagcccc gcctcagcgg ggctcggtgc cggcgatgaa
    22741 cgcctccagg cgggcgcggc cctcggtgtc ctcgcgctgc tccggcgggg acttcatcag
    22801 gtaggccgcg gcggagagca gcgggccgcc gacgccgcgg tccttggcga tcttcgccgc
    22861 gcggatggcg tcgatgatga tgccggcgct gttgggcgag tcccagacct cgagcttgta
    22921 ttcgaggttg aggggcacgt cgccgaaggc gcggccctcg aggcggacgt aggcccactt
    22981 gcgatcgtcg agccaggcga cgtagtcgga cgggccgatg tggacgttgc ggtcctcctt
    23041 cttgccggcc agcggaccgt tgaggttgga ggtgacggcc tgggtcttgg agatcttctt
    23101 cgactccagg cgctcgcgct cgagcatgtt cttgaagtcc atgttgccgc cgacgttgag
    23161 ct
//
LOCUS       DAOPJG010000025        22787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2397_length_22787_cov_7.431330, whole genome shotgun sequence.
ACCESSION   DAOPJG010000025 DAOPJG010000000
VERSION     DAOPJG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 22787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22787
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2397_length_22787_cov_7.431330"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..683)
                     /locus_tag="PKU75_04115"
     CDS             complement(198..683)
                     /locus_tag="PKU75_04115"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HPF80094.1"
                     /translation="MQFRTEMRYPVPPAAVYAMLADPGFREQVCVDQDVISADVDITS
                     NGTGLSVRIDQVQPTQGVPSFAKPVVGETSRAIQIEEWPDESSASLEIKTPGKPATMK
                     GTITLSPEGKGTLEVVELNVKVSVPLVGGRIEKILADLVRRSTAAEQETGLRWLGAGG
                     S"
     gene            748..2259
                     /locus_tag="PKU75_04120"
     CDS             748..2259
                     /locus_tag="PKU75_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HPF80095.1"
                     /translation="MSEPPARVPIPVHDQTWLFMDKPNNLMYINALLWMRSTPDFADL
                     VEVIEERVVGKHPVMGCRAVQDGGAWFWEPDPDFDISRHVTQRRMPKGSTKADVQDHL
                     STCFGTPFPSGRPLWSMELLTGIGDGHESVLFIRIHHGITDGIRSTQLLLNMCDHDAG
                     EHSVARVGDRHAPAGPLDLFEFVGRHVVHDAGDLARATSPVGAPDAMKQLTRLPHAVS
                     ETARRVIRRPGTIRQVPGEVVEGLASFTAEDNTFVNTSRAVFRLVFEPRSPKLAWSGT
                     PGLEKKVGWVDDIPLDEVKALAHDRHATVNDVLMSSVSRALTTYLAEEGTPVTGGALN
                     WLVPISLAPLDAELPPDLGNHFSLVMFRMPVGINDPIDALRATRSMMNRTKHSLEPHV
                     AYTILQTLALGPRMLSAGVRNYLADKCVGQLTNVPGPQSPLNLAGARVESVLGWVPLS
                     GDQTLGLCIFTYAGRIGIGISADAALVRDPQRIADLVAESLDDLTNGSLAGRY"
     gene            complement(2256..2813)
                     /locus_tag="PKU75_04125"
     CDS             complement(2256..2813)
                     /locus_tag="PKU75_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF80096.1"
                     /translation="MALQDAILVTLANGESSGYDLAKSFDVTVANYWTATAQQLYREL
                     DKMAAEGLVSARVVEQERRPNKRVFALTEAGWTALREMTTRTPKPTAIRDELLVQVEA
                     IDHGSEDTVRAAITAKRDASHEKLARYLRRRAAMLDGRDEAAYLRQARRLGPYLTLAR
                     GIAFEEENIAWCDWVLAVTDPGDGA"
     gene            complement(2818..3231)
                     /locus_tag="PKU75_04130"
     CDS             complement(2818..3231)
                     /locus_tag="PKU75_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPF80097.1"
                     /translation="MKTFREAVESRDLDAVEALLADDIVFRSPVVHTPYPGKAITAAI
                     LRHVIEVFEDFHYVRELSDGPNHALIFQAKVDGLDITGCDFLVMNEEGKIADFMVMTR
                     PFKATQALMAHMGARFDQIKADALSAMAIGRSGEG"
     gene            complement(3260..5284)
                     /locus_tag="PKU75_04135"
     CDS             complement(3260..5284)
                     /locus_tag="PKU75_04135"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HPF80098.1"
                     /translation="MTSSTAYPHLLAPLDLGFTSLPNRVIMGSMHVGLEEAPGGFERM
                     AAFYAERARGGVGLIVTGGISPNEAGRPYPGGAMLTTPDEAEKHRLITAAVHAEGGKI
                     AMQILHFGRYAYHPDLVGPSPIQAPISPFPPHELTGAEIEQTIRDYARCAQLAREAGY
                     DGVEIMASEGYFINEFIAAETNKRTDEWGGSYENRMRLPVEIVKAVRAAVGEDFIIIF
                     RLSMLDLVPGGSTFEEVVRLGKEIEAAGVTIINTGIGWHEARIPTIATPVPRGAFAWV
                     TKKLMGSVSVPLVTTNRINTPERAEELLAGGYADLVSMARPLLADPDFVAKTAAGAPE
                     TINTCIGCNQACLDHTFAMKITSCLVNPRACHETELTLAPTRLRKRVAVVGAGPAGLA
                     AATTAAARGHAVTLYDAADRIGGQLNVAREVPGKQEFDETIRYFRNRLEALRVDVRLG
                     AMASAADLAEYDEVVIATGVTPRVPDLQGVDHPKVLGYLDVLAGKAPVGEKVAILGAG
                     GIGFDVAEYLTDSGADTSRDIPAFLAAWGVDADHLTPGGLTEPARHTSPRKVTLLQRK
                     GSKVGANLGKTTGWIHRTELKNRGVEMIPGATYDRVDDAGLHFTVMGHEHVLDVDNVV
                     LCTGQEPNRRLYDELAAAGVTTHLIGGADVAAELDAKRAIDQGTRVAAEI"
     gene            5366..6142
                     /locus_tag="PKU75_04140"
     CDS             5366..6142
                     /locus_tag="PKU75_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPF80099.1"
                     /translation="MVAYFERTGPTSFCATEHVSGAWEVSEQHIAPSLGLIAHCIERD
                     RDARRNDGLVIGRLSYDILGTVPVADVEVETRVLRPGRTIELAEATMTHAGRAIVVAR
                     AWLMATRDTSSVAASPLPGIPGPDQTPVWPASEVWPGGFIESVEVRRAQDEPGRAHFW
                     VRTGLPLVDGEEVSPLARVAGLLDISNGMTVRHDPRQVAFPNVDLTAHFFADPDPDWI
                     GFDTSVSFGASGIGVTTSVLHAPAGPIGVSAQFLTVRPLG"
     gene            complement(6105..6821)
                     /locus_tag="PKU75_04145"
     CDS             complement(6105..6821)
                     /locus_tag="PKU75_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPF80100.1"
                     /translation="MDIILAGGLWLDGRAWDAVLPGLEARGHRGVPVTLPGQGDGNPT
                     ATLDDQLEAILAKVDAAADPPLVVGHSAACTLAGLTADRRPDAVAAIALIGGFPSTAG
                     DQYANFFPMENGGMAFPGWEPFEGPDSGDLSTEMKAEIEVRAVPVPEGVATAVVTYGD
                     ERRHTVPTFLICPEFSPKQAQEWMDSGEVPELAAQAPTLVDIDSGHWPMFSAPEALAE
                     VLAGIADSLSPAGAPSGTAR"
     gene            6916..7878
                     /locus_tag="PKU75_04150"
     CDS             6916..7878
                     /locus_tag="PKU75_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPF80101.1"
                     /translation="MPSANPTARALRALDAISSRPGITATELGAALDVTERAARRYVQ
                     TLRDADIPIESVRGPYGGYRLGRGLRLPPLVFSATEALGLVMATLDGHHAAADPDDPV
                     GSALGKIIRALPEAVGRPAATMRANAVAAPDRHRPRPDPAITAALVAAVAASQRVTVD
                     YHARPDRSWRTDVDPWAVVVRHGRWYLLCHSHAADAIRTYRVDRIASVEAGGETFSPP
                     ADLDPVAFLEEHLGSGWEFETRVVFDAPIEELRHWVGGPMGRLEAIDADHTRLTGSTS
                     NALAYAGEWLAGIPFPFHVEGGPELITATRELAAKLAAAAHTAQ"
     gene            8026..9234
                     /locus_tag="PKU75_04155"
     CDS             8026..9234
                     /locus_tag="PKU75_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80102.1"
                     /translation="MTRFRFGVVASPLHDPADVERIVAPVTERLAGLGGVRDDDPSAG
                     PDQPYAVVVATGGTEAEVLHLLARRPLTTPREPVILVTHRLHNSLPASLEALARLRQD
                     GRHGRIVQSEDLDDLTTMIADIVALQRLRRVRLGLVGKPSEWLVASVPDRVGVRERWG
                     IDLVDVDIAPVIADHQAAPRAGGPVAVKFSRRRPEPSDELEKASALHPALLTAIDDAQ
                     VDAVTVRCFDFLGDLATSGCVALAELNDTGTVAGCEGDIAGAVAMLIARHLLDQASWI
                     ANPSVIDRAAGRLVLAHCTVAPSLVEDLELHTHFESGIGIGLRGTFAPGPVTLLRIGG
                     RELEGHWFADGEILAAGDAPDLCRTQVTLDVDPERLELLLTAPLGNHIVMVRGHHRDR
                     LERWWHLAQA"
     gene            9273..10751
                     /locus_tag="PKU75_04160"
     CDS             9273..10751
                     /locus_tag="PKU75_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HPF80103.1"
                     /translation="MTAPLLLADYATALPELVRPWQPLPEPTQPGAQPPEHVAVNEAL
                     ASKLGVDLGWVRSREGLNVLGARAILPGSTPVAQAYAGHQFGGFSPLLGDGRAVLLGE
                     LRDGAGRLRDLALKGSGRTTFARGGDGRAVLGPVLREYLMGEAMHALGIPTTRALTAI
                     TTGDRVFRDGIMQPGAVLARVAASHLRVGTFEVVARSMEPEASAALLPRLVTYAVRRH
                     YPDLAGSDAEALGLLDAVVAAQAELIARWSSVGFVHGVMNTDNTTISGETIDYGPCAW
                     LETYDEDAVFSSIDTGGRYRFGYQASIGLWNLSRLGECLLPLIDDDPERAVAGATGVL
                     ETFAGRYRDHYLALMADKLGLARDSAQAGPVVMTLLESMAAARADYTLTLRRLADYLR
                     GDEAPDIPGVEHDWLGRWTAILPAGRAETAAAMDAVNPLYVPRNHLVDAALTAAGDGD
                     LAPFERLLAGVSEPYAVRPGLDDLAAPAPPGFTERHVTYCGT"
     assembly_gap    10762..10861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10887..11255)
                     /locus_tag="PKU75_04165"
     CDS             complement(10887..11255)
                     /locus_tag="PKU75_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPF80104.1"
                     /translation="MQKFGEPVEPVARGADPGGPAATGGIAGLHVAVTTDRARLAGHP
                     MLAHLAVESGRAQHVDIAPVERIVGTTGAGDTATAGFLDALLGAVSAATAGERPAALA
                     ARHISGRPLIWPTGVTCRSR"
     gene            complement(11453..12403)
                     /locus_tag="PKU75_04170"
     CDS             complement(11453..12403)
                     /locus_tag="PKU75_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPF80105.1"
                     /translation="MPVRESFPIGGVRVRAGSARELSLPITRMVTGAEVSVPVRIVHG
                     REDGPLVWLTAATHGDEVVGVEVIREVLAGLSPKTLRGTLVAVPIVNVLGFMAGDRYL
                     PDRRDLNRSFPGSARGSLASRIAHLLMSEVIRPGSVGIDLHTGAFGRTNLPQIRCDLD
                     DESTRRLALAFGAPVAFHAKLRDGSLRFAARERSATVLLYEGGEAWRLDDWAVSAGVR
                     GVRRVLADLDMTDPLEPVAPEPVQECRSSGWVRARHTGILRLDVALGQHVTVGERLGA
                     LADSYGKTLRLVRADRAGIVIGRALTPLVNRGDALVHIAQ"
     gene            complement(12406..12882)
                     /locus_tag="PKU75_04175"
     CDS             complement(12406..12882)
                     /locus_tag="PKU75_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HPF80106.1"
                     /translation="MHEPDHPPIVAGWREWVTLPSLKVRWIKAKLDTGAKSSSLHAFD
                     IEEFEKNGRPWVRFAIHPWQRSQAESVIVTSRVIDRRIVRSSSGHAEERIAVPMRVRL
                     VGRVITVEMTVCNRDEMGFRMLLGREALAQGYLVDAAQSYVGGLPRRSTRRKNWGR"
     gene            12908..14087
                     /locus_tag="PKU75_04180"
                     /pseudo
     CDS             12908..14087
                     /locus_tag="PKU75_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
     gene            complement(14188..15639)
                     /locus_tag="PKU75_04185"
     CDS             complement(14188..15639)
                     /locus_tag="PKU75_04185"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF80107.1"
                     /translation="MTAQPSPTTVRPAARPRVVIVGSGFGGLFAARALRRADVDVTLI
                     AKTTHHLFQPLLYQVATGILSQGEIAPSTREVLARQQNATVLLGEVTGIDLEARTVTA
                     HALEESFTQPYDHLIVAAGAAQSWFGHDEFAEFAPGMKSIDDALELRGRIFGAFELAE
                     VAAAEGRHADVEALMTFVVVGAGPTGVEMAGQLVELSKRTLAHDFRHITTKDARIILL
                     DAAPAVLGSFHPSLQSKTKASLEKLGVDVRLGAMVTDVDELGITVKSTGRDGDPFEER
                     IPAVTKVWAAGVSASPLAKLLADQVGVEVDRAGRIPVQDDLTLPGHPEVHVVGDMASL
                     HNLPGVAQVAIQGARHSVDEIEKALAGKPTGAKFEYFDKGSMATISRFRAIAQIKSIR
                     LTGFIAWLAWLAVHLVYIIGFKSRLTTLISWFVSFIGRGRSERTITQQQVFGRLALER
                     ARREGDQGTSARTPHEVSSLLRRSGTSRGPRPD"
     gene            complement(15720..17351)
                     /locus_tag="PKU75_04190"
     CDS             complement(15720..17351)
                     /locus_tag="PKU75_04190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF80108.1"
                     /translation="MPVATMTFLGAAQTVTGSKYLLEIGGRRILVDAGMFQGEKQWRL
                     KNWEDFPVPPDSISDIILTHAHLDHCGYLPALVKHGFDGPVWCTAGTKDLAAIVLRDA
                     GFLAEREARDAAQGGWSKHNPPLPIYTPADVERTLPLLTPVDFDADADLGDDIWVRFT
                     RAGHILGSSSVTVRMPAPDGMPAPSVLFSGDLGRHDHPVLRPRDIPPGSPVVVIESTY
                     GDREHPEPVNLPHEALADVIRRTVQRGGSVLIPAFAIDRTEVVMRILSGMREDKRIPD
                     VPIYINSPMATAGLEVYRRDGGELRADLDLSDFARMSNVHEVRSAEESMELTRGRGKG
                     PSIIISSSGMVNGGRVLHHLERMLPDDRNAIVLTGYQSVGTRGRALMDGATQLKMHGQ
                     YIPVRAEIVVDREFSVHADASDLIDWLRDLQPQPGIVYVTHGEEESSEKLAARIHAEL
                     GLVAVAPGYAERVVLDAVAAPPVAAPAAATPSAPAAPEGAAEEVAAVAVPTPAALAGL
                     DLTGARITSDLTASSGQDGEIVLDGTITIRLRPRT"
     gene            17556..17816
                     /locus_tag="PKU75_04195"
     CDS             17556..17816
                     /locus_tag="PKU75_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPF80109.1"
                     /translation="MELDPTEMDAILKRLKRAQGQIGGIIKMIEDGRECQDIVTQMAA
                     VSKAIDRAGFAVIALGLRQCIADPEGHQMDVASMEKLFLSLA"
     gene            17918..18082
                     /locus_tag="PKU75_04200"
     CDS             17918..18082
                     /locus_tag="PKU75_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80110.1"
                     /translation="MCRAVVCKKCGKTTWAGCGQHVDQVMRGVPKAQQCQGHADEPSQ
                     GGFLGKLFGR"
     gene            complement(18233..18409)
                     /locus_tag="PKU75_04205"
     CDS             complement(18233..18409)
                     /locus_tag="PKU75_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80111.1"
                     /translation="MTDMTSTPAETPVPVADLSRAPLPTAGTLRKRKSVPIQLTRFAV
                     FNTRIMRMVLKGDH"
     gene            complement(18415..19872)
                     /locus_tag="PKU75_04210"
     CDS             complement(18415..19872)
                     /locus_tag="PKU75_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF80112.1"
                     /translation="MADIVILGAGVSGHTAALHLSRLLPKGHTVTVVSPNSQWNWIPS
                     NIWVGVGKMSKKDVVFPLAPVYAKKGVQFRQAKAVAIHPRGDAGSQRPAVDIVHTSPA
                     RDGETERIRYDYLVNATGPQLKFQMTEGLGPHGGHTVSVCTADHAIEAAAALEASIAK
                     LKSGQRQTLVVGMGHGTCTCEGAAFEYVFNVDHELREAGVRDLARVVYLTNEAELGDF
                     GVGGMTFDDMGFETSSELWTGSLFRERGVEAVLGVHVTTVEPGVVHFETLDGQMHSLA
                     FDFAMLLPPFGGVPLAAYDRDGSDITSELFAPSGFMKVDADYSGKPYEQWKASDWPKT
                     YLAQGYDNIWAVGIAFAPPHQISKPRKTPNGTMIAPSPPRTGMPSGVMGKTAALSIVD
                     RIKHGPGAKAHEASMANMGAACIASAGAGLRTGSAAAMTMMPIVPDYDRYATGRDLKD
                     TRGELGLSGHWVKLMLHYLFIHKAKGRLGWQFIPE"
     gene            complement(19977..20732)
                     /locus_tag="PKU75_04215"
     CDS             complement(19977..20732)
                     /locus_tag="PKU75_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPF80113.1"
                     /translation="MLLLTLTLALLVGVTLGLMGGGGSILMVPLLVYVARLAPGTAIA
                     ASLFVIGVTAIAAVLPHARGGRVQWRAGAVLGVAAMAGAYAGGRLAAYVPEWVLLATF
                     ALLMLATAIAMIRGRRDETGEAASGALAVGPVLLAGVGVGLLTGFVGAGGGFLIVPAL
                     TLFARLPMRQAIGTSLLVIAMNSFAGLAGHLASVSVPWATVLAVTAAAVVGSVVGGRL
                     TGRVHPAHLRRGFGWFVLAMGLCVLTETVLTHA"
     gene            complement(20743..22149)
                     /locus_tag="PKU75_04220"
     CDS             complement(20743..22149)
                     /locus_tag="PKU75_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPF80114.1"
                     /translation="MILTQYYLDCLSQASYLVADETTRLAVVVDPRRDIDDYLADAKE
                     ADLTIVGILNTHFHADFVSGHLELAKATGAWIGYGDQAKADFDIRAFADGERLSLGDV
                     TLEIMHTPGHTPESISVLVYEHADDEVAYAVLTGDALFIGDVGRPDLLASIGFTADEL
                     GSMLYDSIHTKLMGLPDPVRVFPAHGAGSACGKNLSTEKQSTIGAQRATNYACQPMTR
                     EQFLDVVTEGQPSAPGYFVYNATVNRQERSILDVDHHVSALSENEIRDALAGGAIVTD
                     ARPNAEYAVAHLRGSLNVPSDGRLAETVGMVLGPQDRVILIAPDGQEQEVATRFHRIG
                     YDNVLGFIPQPEAYFLAHPDDMAVASRLSVAEADAALESVPGLQLLDIRNPGEVAGGM
                     IPGASHIPLAELGRRAAELDKERPVLAYCAGGWRSSVGSSLLRAKGFADVSDILGGYN
                     AWRSAHDLEPASCATTTA"
     gene            22245..22724
                     /locus_tag="PKU75_04225"
     CDS             22245..22724
                     /locus_tag="PKU75_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPF80115.1"
                     /translation="MTRHLTSTAAALTLAIAALAGCSGNDPAPSASAASPSASSASGA
                     GAASATAAPANGATLDAAAFAAALKAPHTTILDVRTPAEFASGHLEGAVNADIEGDFA
                     AAIADLDKAAPYAVYCRSGNRSAIAVQAMQQAGFTATYHLDGGITAWQAAGGTVVTG"
BASE COUNT         3473 a         7706 c         7902 g         3606 t
ORIGIN      
        1 tgcgcttggc gatctggctc agggattggc tgggccgttc tcgcgtgaac gtgccgagca
       61 cggccagggt cttgcccgct gccgtcagct gatccgccac ccggccagtg tggcatcgtc
      121 gacgtccgac gggtgcgatc tgtgacgggg ccccagtgca tcggtcccct gcccggaccg
      181 atcccgctca tccgggctca ggagccgcca gcccctagcc agcgcagacc cgtctcctgc
      241 tcggcagcag tgctgcggcg cacgaggtcg gccaggatct tctcgatccg gccgccgacg
      301 agcggcacgg ataccttgac gttgagttcg acgacctcga gggtgccttt gccttcgggg
      361 ctgagggtga tggtgccctt catggtggcg ggtttgccgg gggtcttgat ctccagggac
      421 gccgacgatt cgtccggcca ttcctcgatc tggatcgcgc gggaggtctc tccgacaacg
      481 ggtttggcga aggacgggac gccctgggtc ggctgcacct ggtcgatgcg cacgctcaat
      541 cccgtcccat tggaggtgat gtcgacatcc gccgagatga cgtcctggtc gacacaaacc
      601 tgttcgcgga accccgggtc ggcgagcatc gcatagaccg ctgcgggcgg aacgggatag
      661 cgcatctcgg tgcggaactg cataccctcc actgtgccgc cccgcgcgca ctttcgcagg
      721 cccggccgga cgtgatctag tggacatgtg agtgaacccc cggcacgggt gccgatccct
      781 gtccacgacc agacctggct gttcatggac aagcccaaca acctgatgta catcaacgcg
      841 ctgctctgga tgcgctcgac gcccgacttc gcggacctcg tcgaggtgat cgaggagcgg
      901 gtcgtcggca agcatccggt gatgggctgc cgggcggtgc aggacggcgg cgcctggttc
      961 tgggagcccg accccgactt cgacatctct cggcacgtca cgcagcgccg gatgccgaag
     1021 ggcagcacga aggccgatgt gcaagaccac ctgtcgacgt gcttcggcac cccgttcccg
     1081 tccgggcggc cgttgtggag catggaactg ctcaccggca tcggcgacgg ccacgagtcg
     1141 gtgctgttca tccggatcca ccacggcatc accgacggca tccgctcgac gcagctgctg
     1201 ctgaacatgt gcgaccacga cgccggcgag cacagtgtcg cccgggtcgg cgaccggcac
     1261 gcgccagctg gcccgctcga tctgttcgag ttcgtcggcc ggcacgtcgt gcacgacgcc
     1321 ggcgacctgg cgcgcgcgac gagcccggtc ggggcgccgg acgccatgaa acagctcacg
     1381 cggctcccgc acgcggtcag cgagaccgcc cgccgggtga tccggcgccc cgggaccatc
     1441 cgccaggtgc cgggtgaggt cgtcgagggg ctggcctcct tcaccgccga ggacaacacc
     1501 ttcgtcaaca ccagccgcgc cgtcttccgg ctggtcttcg agccgcgcag tcccaaactc
     1561 gcctggtccg gcacgcctgg cctggagaag aaagtgggct gggtcgacga catccccctc
     1621 gatgaggtca aggccctggc acacgaccgg cacgcgaccg tcaacgacgt gctcatgagc
     1681 agcgtctccc gcgccctgac gacctatctg gccgaagagg gcacccccgt gaccggcggc
     1741 gctctcaact ggctcgtgcc gatctccttg gcccccctcg acgccgagtt gccgcccgac
     1801 ctcggcaacc acttcagcct cgtgatgttc cggatgccgg tcgggatcaa cgacccgatc
     1861 gatgccctgc gcgccacccg ctcgatgatg aaccggacca agcattcgct cgagccgcac
     1921 gtcgcctaca ccatcctgca gacgttggcg ctcggccccc ggatgttgtc agccggtgtc
     1981 cgcaactatc tcgccgacaa gtgcgtcggc cagctgacca atgtgccggg cccgcaatca
     2041 ccgctcaacc tcgccggggc gcgcgtggag tcggtcctcg ggtgggtgcc cctctccggg
     2101 gatcagaccc tcggtctgtg catcttcacg tatgccgggc gcatcggcat cgggatcagc
     2161 gccgacgccg ccttggtccg cgacccgcaa cggatcgccg acctggtcgc cgagtcgctc
     2221 gacgatctca ccaacggctc cctggccggc cggtactagg caccgtcgcc ggggtcggtc
     2281 acggcgagca cccagtcgca ccaggcgatg ttctcctcct cgaaggcgat gcctcgcgcg
     2341 agcgtgagat aggggccgag gcgccgggct tgccgcaggt atgccgcctc gtcccgcccg
     2401 tcgagcatgg cggcgcggcg gcgaagatag cgcgcaagtt tctcatgtga cgcatcacgt
     2461 ttcgccgtga tggccgctcg gacggtgtcc tcgctgccgt ggtcgatcgc ctcgacctgg
     2521 accaggagtt cgtcacggat ggcggtgggc ttcggtgtcc gggtcgtcat ctcgcgcagc
     2581 gccgtccagc cggcctcggt cagggcgaag acgcgcttgt tcggccggcg ctcctgctcg
     2641 acgacgcgtg ccgacaccaa tccctcggca gccatcttgt ccagctcgcg gtagagctgc
     2701 tgggcggtgg ccgtccaata gttcgcgacc gtgacgtcga aggacttggc caggtcatag
     2761 cccgacgact cgccattggc cagagtcacc aggatcgcgt cctgcagtgc cacccgatca
     2821 gccctcgccg ctgcggccga tggccatcgc ggacagggcg tcggccttga tctgatcgaa
     2881 ccgggctccc atgtgcgcca tcagggcctg ggtcgccttg aaggggcggg tcatcaccat
     2941 gaagtcggcg atctttccct cctcgttcat gacgaggaag tcgcacccgg tgatgtcgag
     3001 accgtcgact ttcgcctgga agatcagggc gtggttcgga ccgtcggaca gctcgcgcac
     3061 gtagtggaag tcctcgaaga cctcgatgac gtggcgcagg atggccgcgg tgatcgcctt
     3121 gcccgggtag ggcgtgtgca ccaccgggct gcggaagacg atgtcgtcgg cgagcagcgc
     3181 ctcgaccgcg tccaggtcgc ggctctcgac ggcctctcgg aaggttttca tggtgaaact
     3241 ccttgtcctg gtgcctatct cagatctcgg ccgcgacgcg agtgccctga tcgatggcgc
     3301 gtttggcgtc gagttcggcg gcgacgtcgg cgccgccgat gaggtgggtg gtcacaccgg
     3361 ccgcggccag ttcgtcatac agccggcggt tgggttcctg cccggtgcac aggacgacgt
     3421 tgtcgacatc gagcacgtgc tcatgcccca tgaccgtgaa gtgcaggccg gcgtcgtcga
     3481 cgcggtcgta tgtcgcgccg gggatcatct ccacgccgcg gttcttcagt tccgtccggt
     3541 ggatccagcc cgttgtcttg ccgagattgg cgcccacctt ggaacccttg cgctgcagca
     3601 gtgtcacctt ccggggggag gtatgccgcg ccggctcggt gagcccgccg ggcgtcaggt
     3661 ggtccgcatc gacaccccat gcggcgagga aggcgggaat gtcccggctg gtgtcggcgc
     3721 cggagtcggt gaggtactcg gcgacgtcga atccgatgcc gccggcgccg aggatggcga
     3781 ccttctcgcc gacgggcgcc ttgccggcca gcacgtcgag atagccgagg accttcgggt
     3841 ggtcgactcc ctgcaggtcc gggacgcgcg gcgtgacccc ggtcgcgatg acgacctcgt
     3901 cgtattccgc aaggtcggcc gcgctggcca tggcgcccag gcgcacgtcg accctcagcg
     3961 cttccagccg gttgcggaaa tagcggatcg tttcgtcgaa ctcctgcttg cccggcacct
     4021 cccgcgcgac attgagctgc ccgccgatcc ggtcggccgc gtcatacagg gtcaccgcgt
     4081 gaccccgcgc ggccgccgtc gtcgcggcgg ccaacccggc cgggcccgcg ccgacgaccg
     4141 ccacgcgctt gcgcaatcgt gttggcgcca aggtcaattc ggtctcgtgg caggcgcgcg
     4201 gattgaccag gcaggaggtg atcttcatgg cgaaggtgtg gtcgaggcag gcctggttgc
     4261 agccgatgca cgtgttgatc gtctcgggcg caccggcggc agtcttggcg acgaagtcgg
     4321 ggtcggcgag cagcgggcgc gccatcgaga ccaggtcggc atacccgccc gcgagcagtt
     4381 cttcggcgcg ttcgggtgtg ttgatccggt tcgtcgtgac cagcggcacc gataccgaac
     4441 ccatgagctt cttcgtgacc cacgcgaagg cacctcgcgg gactggggtg gcgatggtgg
     4501 ggatgcgcgc ctcgtgccag ccgatgcccg tgttgatgat cgtgacgcct gcggcctcga
     4561 tctccttgcc gagccgcacg acctcctcga aagtcgaacc gcccggcacc aggtcgagca
     4621 tcgagagccg gaagatgatg atgaagtctt cgcccacggc tgcgcggacg gccttgacga
     4681 tctcgacggg cagccgcatc cggttctcgt aggagccgcc ccactcgtcc gtgcgtttgt
     4741 tggtctcggc ggcgatgaac tcgttgatga agtagccctc cgaggccatg atctccacgc
     4801 cgtcgtagcc ggcctcccgg gccagctgcg cgcaccgggc gtagtcgcgg atcgtctgct
     4861 cgatctccgc gccggtcagt tcgtgcggcg ggaaggggct gatcggcgcc tgaatcgggc
     4921 tgggccccac cagatccggg tgataggcat agcggccgaa gtgcaggatc tgcatggcga
     4981 tcttgccgcc ctcggcgtgc accgccgcgg tgatgaggcg gtgtttctcg gcctcgtccg
     5041 gcgtggtcag catggccccg cccgggtagg gacgccctgc ttcgttgggg gagatgccgc
     5101 cggtgacgat gagcccgacg ccacctcggg cccgctcggc atagaaggcc gccatcctct
     5161 cgaatccgcc aggagcctcc tccaggccca cgtgcatcga ccccatgatc acccgattgg
     5221 ggaggctggt gaagcccagg tcgagcgggg cgaggaggtg ggggtaggcc gtcgagctag
     5281 tcatgtttgc cagtatgcaa caaatttaat attcggggcc agagggtggc cactatggcc
     5341 tgtgccactg ccgccatacc ctgatgtggt ggcctatttc gagcgcaccg gaccgacgag
     5401 cttctgcgcg accgagcatg tcagcggcgc gtgggaggtc agcgagcagc acatcgcccc
     5461 gtcgctgggg ctgatcgcgc attgcatcga acgggaccgg gacgctcggc gcaacgacgg
     5521 ccttgtcatc ggccgcctct cctacgacat cctcggcacg gtgcccgtcg ccgatgtcga
     5581 ggtggagacc cgcgtgctgc gccccggtcg caccatcgaa ctggccgagg caacgatgac
     5641 ccacgccggc cgcgcgatcg tcgtcgcgcg agcctggctg atggcgaccc gcgacacgag
     5701 ctcggtggcg gcgagcccgc tgcccggcat acccgggcct gaccagacgc cggtgtggcc
     5761 cgcgagcgag gtgtggcccg gtggcttcat cgagtcggtg gaggtgcggc gcgcccagga
     5821 tgagccgggc cgcgcccact tctgggtccg caccggcctg ccgctcgtcg acggtgagga
     5881 ggtgagccca ctcgctcgcg ttgcgggttt gctggacatc tccaacggca tgacggtgcg
     5941 tcacgacccg cgccaggttg ccttccccaa tgtcgacctc accgcgcact tcttcgccga
     6001 ccccgatccg gactggatcg gcttcgacac ctcagtctct ttcggcgcca gcggaatcgg
     6061 cgtgacgacg agtgtcctgc acgcaccggc cggcccgatc ggcgtcagcg cgcagttcct
     6121 gacggtgcgc ccgctgggct gagactgtcg gcgattccgg cgagcacctc ggcgagcgct
     6181 tccggcgcgc tgaacatcgg ccagtgcccc gagtcgatgt cgacgagggt cggcgcctgg
     6241 gcggccagtt ccgggacctc gccgctgtcc atccactcct gtgcctgctt gggactgaac
     6301 tccgggcaga tgaggaatgt ggggacggta tgccgtcgct cgtcgccgta ggtgaccacg
     6361 gccgtcgcga ctccctccgg caccggcacc gcgcgcacct cgatctccgc cttcatttcc
     6421 gtgctcaggt cgccggagtc ggggccctcg aacggttccc acccggggaa tgccatgccg
     6481 ccgttctcca tggggaagaa attcgcatac tgatcgccgg ccgtgctggg gaatccgccg
     6541 atgagggcga tcgcggccac ggcgtcggga cgccggtccg ccgtgaggcc cgcgagcgta
     6601 caggcggccg agtggccgac gacgagcggc gggtcggccg cggcatcgac cttggcaagg
     6661 atcgcctcga gctgatcgtc gagggtggct gtggggttgc cgtcgccctg acccggcagg
     6721 gtcacgggca cgccgcggtg gccacgggcc tcgagccccg gaagcaccgc gtcccaggcg
     6781 cgaccgtcga gccacagtcc gccggcgaga atgatgtcca tggttggtcc ttctccgtcg
     6841 ttgaggtgat gacgtcgagg accctagaga cgattccgga cagaaacgtt ccgcattgtc
     6901 cgtaacctgg tgggtatgcc gtccgccaac cccaccgcac gcgcgctgcg cgcgctggac
     6961 gcgatctcct cccgcccggg catcacggca accgaactcg gtgccgcgct ggacgtgacc
     7021 gagcgcgccg cccgccgcta cgtgcagacc ctccgcgacg ccgatatccc tatcgagtcg
     7081 gtgcgcggcc cttatggcgg ctaccgactg gggcgcggat tgcggcttcc cccactggtt
     7141 ttcagtgcca ccgaagcgct cggcctcgtc atggccaccc tcgacggaca ccatgcggcc
     7201 gccgatcccg acgaccctgt cggttcggcg ctgggcaaga tcatccgggc gctgcccgag
     7261 gccgtcggcc ggccggcggc cacgatgcgc gccaatgccg tcgccgcccc cgaccggcac
     7321 cggccccggc ccgacccggc gatcacggcg gccctcgtgg cagccgtggc cgcctcccag
     7381 cgcgtcacgg tggactatca cgcgcggccg gatcgttcct ggcggaccga cgtcgacccg
     7441 tgggccgtcg tcgtgcgtca tggccggtgg tatctcctgt gccactccca tgccgccgac
     7501 gccatccgca cctaccgcgt cgatcgcatc gcatccgtgg aggccggcgg cgagacgttc
     7561 agcccgccgg ctgatctcga ccctgtcgcc ttcctggagg agcacctcgg ctccggctgg
     7621 gaattcgaga cccgcgtggt cttcgacgcg cccatcgagg agctgcggca ctgggtcggg
     7681 ggacccatgg gccgcctgga ggcgatcgac gccgaccaca cccgcctcac cgggagcacc
     7741 agcaacgccc tggcgtatgc cggggagtgg ctggccggca tacccttccc cttccacgtg
     7801 gagggtggtc ccgaactaat cactgccaca agagaactgg cggccaaact cgccgcggca
     7861 gctcacaccg cccagtagtt gtcgtgttcg ctcatgaagt cgaagtactc ctgcggcgac
     7921 atcgcgtcga gatcttcacg cgcaagtctc tcaggatgac gacgtgagcc gatgcactgc
     7981 gatcctggct tacgccacac ccgcagtcgg tggtggggtg gaggtatgac gcgtttccga
     8041 ttcggtgttg tcgcttcgcc tctgcacgat cccgcggacg tggagcggat cgtcgcgccc
     8101 gtcaccgagc gactggcggg cctgggaggc gtgcgtgacg atgacccgtc ggccggaccc
     8161 gatcagccgt atgcggtcgt cgtcgccacc gggggcaccg aggcggaggt gctccatctg
     8221 ctcgcacgcc ggccgctgac tacgccacga gagccggtga tcctggtgac gcatcgcctg
     8281 cacaacagtc tgccggcgtc gctggaggcg ttggcgcggt tgcgtcaaga cggtcggcac
     8341 ggtcgaatcg tccagagcga ggacctcgac gacctcacga cgatgatcgc cgatatcgtt
     8401 gcgctgcaac gcctccggcg ggtccggctg ggcttggtgg gcaagccgtc ggagtggctc
     8461 gtcgccagcg tgccggatcg ggtcggcgtc cgcgagcgct ggggcatcga cctggtcgac
     8521 gtcgacatcg cgccggtgat cgccgatcac caggctgcgc cgcgcgcggg tgggccagtt
     8581 gccgtgaagt tctcgcgccg ccgccccgag ccctccgacg aactcgagaa ggcctccgcg
     8641 ctgcatccgg ctctcctgac ggcgatcgac gacgctcagg tcgacgccgt caccgttcgg
     8701 tgcttcgact tcctgggcga cctcgccacc tccggctgcg tcgccctcgc cgaactcaac
     8761 gacaccggaa ctgtcgccgg ctgcgaaggc gatatcgccg gggccgtcgc gatgctcatt
     8821 gcccggcatc tgctcgacca ggcctcgtgg atcgccaatc cgtcggtcat cgaccgcgcg
     8881 gccgggcggc tggtcctcgc ccactgcacg gtcgcaccct cgctcgtcga ggatctggaa
     8941 ctgcacaccc acttcgagag cggcatcggc atcggcctgc gcggcacgtt cgcgcccggc
     9001 cccgtgaccc tgctgcgcat cggcggccga gagttggagg ggcattggtt cgccgacggc
     9061 gagatcctcg ccgccggtga tgcaccggat ctgtgccgca cgcaggtcac gctggacgtg
     9121 gaccccgagc ggctggagtt gctgctcacc gcgcctctcg gcaatcacat cgtcatggtt
     9181 cgcggccacc accgcgaccg gctcgaacgc tggtggcacc tggctcaggc ctgaacagca
     9241 gtttcgtgac agccgcgcgt tagcgtggtc gagtgacagc gccccttctg ctcgccgact
     9301 atgccacggc actcccggag ttggtccggc cgtggcagcc gctgcccgag cccacccaac
     9361 ccggggcgca gccgccggag cacgtggcgg tcaacgaggc gctggcatcg aagctcggcg
     9421 tggacctggg atgggtccga agccgggagg ggctgaatgt gctcggtgcc cgggcaatcc
     9481 tgcccggctc gaccccggtg gcgcaggcgt acgccgggca tcagttcggg ggcttctcac
     9541 cgctcctcgg ggacggccgt gcagtgctgc tcggtgaact ccgcgacggc gcaggccggc
     9601 tgcgggatct cgcgctcaag ggctccggcc gcacgacgtt cgctcgagga ggcgacggcc
     9661 gagccgtgct cgggccggtg ctgcgcgaat atctcatggg tgaggcgatg cacgccctcg
     9721 ggatcccgac aacccgggcg ctgaccgcaa tcaccacggg agacagggta tttcgcgacg
     9781 gaatcatgca gcccggcgct gtgctcgccc gcgtcgccgc gtcccacctg cgcgtcggca
     9841 ccttcgaggt cgtggcccgg tcgatggagc cggaggccag cgcggccctg ttgccgcgcc
     9901 tggtgacgta tgccgtgcgc cggcactatc ccgacctggc cggctccgat gccgaggccc
     9961 tcgggctgct cgacgcggtc gttgctgcgc aagcggagtt gatcgcccgg tggagcagtg
    10021 tcggcttcgt ccacggcgtg atgaacaccg acaacacgac gatctccggc gagaccatcg
    10081 actacggccc gtgcgcgtgg ctggaaacct acgacgaaga tgccgttttc tcctcgatcg
    10141 acaccggcgg ccgctaccgc ttcggctatc aggccagcat cggcctgtgg aacctcagcc
    10201 ggctcggtga gtgcctgctg ccgctcatcg acgacgaccc ggaacgagcc gtggcgggtg
    10261 ccaccggcgt cctggagacc ttcgccgggc gctatcgcga ccactacctc gcgttgatgg
    10321 cggacaaact ggggctcgcg cgcgactcgg cgcaggccgg cccagtcgtg atgacgctgc
    10381 tggagtccat ggccgcggcc cgggccgact acacgctgac tctccgccgg ctggctgact
    10441 acctccgggg cgacgaagcc ccggatatcc ctggcgtgga acacgattgg ctgggccgat
    10501 ggactgccat actgcctgcg ggccgcgccg agaccgctgc cgcgatggac gccgtcaacc
    10561 cgctctacgt cccgcgcaac cacctcgtcg acgcggcact caccgccgcg ggcgacggtg
    10621 atctggcacc tttcgagcgg ctgctcgccg gggtcagcga gccgtatgcc gtccgccccg
    10681 gtctcgacga tctcgccgct cccgccccgc ccggcttcac cgagcggcac gtcacctact
    10741 gcggcacgtg acgcccgtgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nccgcccggc ttcaccgagc ggcacgtcac ctactgcggc acgtgacgcc cgtgggccag
    10921 atcagcggcc ggccggagat gtggcgagcc gccagtgcag cagggcgttc gccggccgtc
    10981 gcggccgaga cggcaccgag cagcgcgtcg aggaagccgg ccgttgccgt gtcacccgcc
    11041 ccggtcgtcc cgacgatccg ttcgacggga gcaatgtcga cgtgctgcgc gcggccgctc
    11101 tcgaccgcaa ggtgcgcgag catcggatga ccggcgagtc gggcccggtc cgtggtgacg
    11161 gcgacgtgta gaccggcgat gccgccggtg gccgccgggc cgccggggtc cgcaccgcgt
    11221 gcgaccggct cgaccggctc accgaacttc tgcatacgga ccctcatccg gccatcaccc
    11281 acatcccgac ctccgacgcc ccctccgtca tcggcccgga gtggtatcaa agctgacacc
    11341 ggggtgaagg atcgtccgcg ggctcaggag agggtggtcg aatcagcagg cctagcacgg
    11401 tgtgcagatt gaattacgct ctgccagcgc agattacgtg gccccggcag ggctactggg
    11461 cgatgtgtac gagggcgtcc ccgcggttga cgagcggggt cagggcgcgg ccgatgacga
    11521 tgccggcgcg gtcggcgcgc accagccgca aggtcttgcc gtaggagtcg gcgagggcgc
    11581 cgagccgctc gccgacggtg acgtgctgac cgagggcgac gtcgagccgg aggatcccgg
    11641 tatgccgggc ccgcacccat ccgctggagc ggcactcctg aaccggttcc ggcgcaacgg
    11701 gttccagcgg gtcggtcatg tccaggtcgg cgagcacccg gcgcaccccg cggacgccgg
    11761 cggacaccgc ccagtcgtcg aggcgccagg cctcgccgcc ttcgtacaac agcactgtgg
    11821 cgctccgctc gcgggcggcg aagcgcagcg atccgtctcg gagcttggca tggaacgcga
    11881 cgggcgcgcc gaaggcgagg gcaagacgcc gggtcgactc gtcgtcgagg tcgcatcgga
    11941 tctgcggcag gttggtgcgc ccgaaggcac ccgtatgcag gtcgatcccg acgcttcccg
    12001 gccggatcac ctcgctcatg aggaggtggg cgatgcggct ggcgaggctg ccgcgggcgg
    12061 acccggggaa gctgcggttc aggtcgcgcc ggtcgggcag gtagcggtcg ccggccatga
    12121 agccgagcac gttgacgatc ggcacggcga caagggtgcc gcgtaatgtc ttgggggaca
    12181 acccagccag cacttcgcga atgacctcga cgccgacgac ctcgtcgccg tgagttgccg
    12241 cggtgagcca caccaagggc ccgtcctcgc ggccgtggac gatgcgcacg ggcacgctga
    12301 cctcggcgcc ggtgaccatc cgggtgatcg ggaggctgag ctctcgggcg ctcccggccc
    12361 gcacccggac gcccccgatg gggaaggact cgcggaccgg catcgtcagc gcccccagtt
    12421 cttccggcgc gtggagcggc gggggaggcc gccgacatac gactgcgctg cgtcgacgag
    12481 gtaaccctgc gcgagcgcct cgcgcccgag caacatgcgg aagcccatct cgtcgcgatt
    12541 gcacacggtc atctcgacgg tgatgacccg gccgacgagt cgcacccgca tgggcaccgc
    12601 gatgcgctcc tcggcgtgac cggacgaact gcgcacgatg cgccggtcga tgacccggga
    12661 ggtgacgatc accgattcgg cctgcgagcg ctgccacggg tggatggcga aacgcaccca
    12721 cgggcgtccg ttcttctcga actcctcgat gtcgaaggcg tgcagcgagg acgacttcgc
    12781 cccggtgtcg agtttcgcct tgatccaccg gactttcaga ctcggcaggg tgacccattc
    12841 ccgccacccg gccacgatgg gaggatggtc tggctcgtgc acccgtccat cctcgcagga
    12901 atccggtatg aagctggcca tcttggcgcg tgccccgagg tgttatacga ctgggcggct
    12961 gcgccgcggc cgaccgcggc catacggtca aggtgctcaa caccttgcgc ttcgggatcg
    13021 acctgtccgg gccggaaccg gacctgcagt tccgcggcaa gccgctgtcc cactatgacg
    13081 cgatcctgcc gcggatcggc aactcggtga cctacttcgg cacggcagtg gtgcggcagt
    13141 tcgagcagat ggacgtctac acgccgaaca ccgccaacgg catcgccaac tcgcgagaca
    13201 agttgcgcgc cacgcagatc ctctcgcgcc acaacatcgg tatgccgccg accaccttcg
    13261 tccgcgaccg cgccgacgtg atcccggcga tcgagcgggt cggcggggca ccggtggtca
    13321 tcaagcttct cgaaaggacg caggggatcg gggtgatcct cgcaccggag ctcaaggtgg
    13381 ccgaggcgat catcgagacc ttgcagagca cccgacagaa cgtgctggtc cagcgcttcg
    13441 tcgccgagag caagggccgt gacatccggg ccctcgtcgt cggggatcgc gtggtcgcag
    13501 cgatgcgccg cgtggcgaag ggcgacgagt tccgctccaa cctccaccgc ggcggaagcg
    13561 tcgaaaaggt ctctctggag ccggctttcg aggaaacggc cgtccgcgcg gcacagatca
    13621 tgggactgcg ggtcgccggg gtcgacatgt tggagggcgc ggacggcccg ctggtgatgg
    13681 aggtgaattc ctcacccggg ctcgagggca tcgaggccgc gaccaagctc gacgtggccg
    13741 gcgccatcat cgactacatc gacaaccagg ttgattttcc ggaaatcgat gtgcgccaac
    13801 gccttacggt gtccagaggg tatggcgtgg cggagatcgt cgtgcacgcg gcctccgacc
    13861 tggtgggccg gcccctcgcc gaatccggcc tgcgggacaa agacatctcc gtgctgactc
    13921 tgcaccgggg tgcacgcgtc atacccaacc cgggcaaggg tcacgtgctc gaggacggcg
    13981 accggctgct gtgcttcggc aagctcgagg agatgcgctc gatgatcccc gcccggcgcg
    14041 cccgacgcgt caagaagcta ccgaagcagc cgacccacga gatctgaatt cggccgtctc
    14101 ccaacgactt agcagagcct agtcaaatct gcacactttc ctaggtgtgc tgatttgact
    14161 agcttctgct aggccctgtt gcgaaactca atcgggacgg ggtccccgcg aagtaccgga
    14221 gcgacgaagg agtgaggaca cttcgtgggg tgtcctagca gaggttcctt ggtctccctc
    14281 gcgccgggcg cgctcgagag ccaggcggcc gaagacctgc tgctgggtga tcgtgcgctc
    14341 ggagcggccg cggccgatga agctcacgaa ccagctgatg agggtggtca ggcgcgactt
    14401 gaagccgatg atgtagacga ggtggacggc cagccaggcg agccacgcga tgaagccggt
    14461 cagccggatc gacttgatct gggcgatggc gcggaagcgc gagatcgtcg ccatcgagcc
    14521 cttgtcgaag tactcgaact tcgcgccggt cggtttgccc gccaacgcct tttcgatctc
    14581 atcgacgctg tgccgcgcac cctggatggc gacctgggcg acgccgggga ggttgtgcag
    14641 gctcgccatg tcgccgacga cgtgcacctc cgggtggccg ggcaacgtca gatcgtcctg
    14701 caccggtatg cggcccgccc ggtccacctc gacgcccacc tggtcggcga gcagcttggc
    14761 gagggggctg gccgagacgc cggcggccca caccttggtg accgccggga tgcgttcctc
    14821 gaaggggtca ccgtcgcgtc cggtgctctt gacggtgatt ccgagttcgt cgacgtcggt
    14881 gaccatggcg ccaagccgga cgtccacccc gagcttctcg agcgatgcct tggtcttgga
    14941 ctgcaggctc gggtggaaac tgccgaggac ggcgggcgcg gcgtcgagca ggatgatgcg
    15001 cgcatccttc gtggtgatgt gccggaagtc atgggcgaga gtacgtttgg agagttcgac
    15061 gagttggccg gccatctcca ctcctgtcgg cccggcgccg acgacgacga aggtcatcag
    15121 cgcctcgacg tcggcgtgcc ggccttcggc cgcggcgacc tcggccagct cgaacgcccc
    15181 gaagatccgg ccgcgcagtt cgagggcgtc gtcgatcgac ttcatgcccg gggcgaactc
    15241 ggcgaactca tcgtggccga accacgactg cgccgcgccg gccgccacga tgagatggtc
    15301 gtatggctgc gtgaacgact cctcgagcgc gtgtgcggtg accgtccggg cctccaggtc
    15361 gatgccggtc acctcgccga gcagcacggt ggcgttctgc tgccgtgcga ggacctctcg
    15421 ggtcgacggg gcgatctcgc cctgggacag gatgccggtc gccacctgat agaggagcgg
    15481 ctgaaagagg tggtgggtcg tcttggcgat gagcgtcacg tcgacatcgg cccggcgcag
    15541 cgcacgggcg gcgaagaggc cgccgaagcc ggacccgacg atcaccaccc gcgggcgggc
    15601 ggcggggcgg acagtggtcg gcgagggctg cgcggtcacg tcaggtccaa catcccaggt
    15661 gaggcgagac ttccctctgt cgcgaagatg tcgcgccgat cacagcgtct ccctccttct
    15721 caggtgcggg ggcgcaggcg gatggtgatg gtgccgtcga ggacgatctc gccgtcctgt
    15781 ccggatgacg cggtcaggtc actcgtgatg cgggctccgg tcaggtccag gccggcgagg
    15841 gcggccgggg tgggaaccgc gacggctgcc acctcctcgg ccgcgccctc cggcgcggcg
    15901 ggtgcggagg gagttgccgc agcgggcgcg gccaccggag gagccgccac ggcgtcgagg
    15961 acgacccgct cggcataccc cggagcgacg gcgaccagcc ccaactccgc gtgaatgcgc
    16021 gcggcgagct tctcggagga ctcttcctcg ccgtgggtga catagacgat ccccggctgc
    16081 ggctgcaggt cgcgcagcca gtcgatgagg tcggaggcgt cggcgtggac ggagaactcc
    16141 cggtcgacga cgatctcggc gcgcacgggg atgtactggc cgtgcatctt cagctgcgtg
    16201 gcgccgtcca tcagggcccg gccacgagtg ccgacggact ggtatccggt cagcacgatc
    16261 gcgttgcggt cgtcgggcag catccgctcc aggtggtgca gcacccgccc gccgttgacc
    16321 atgccggagg agctgatgat gatcgacggg cccttcccgc ggccgcgcgt cagctccatc
    16381 gactcctcgg cggatcggac ctcgtggacg ttcgacatac gagcgaaatc ggacaggtcg
    16441 aggtcggcgc gcagctcgcc accgtcgcgg cgatagacct cgaggccggc ggtggccatg
    16501 gggctgttga tatagatcgg cacgtcgggg atccgcttgt cctcgcgcat gccggacagg
    16561 atgcgcatca ccacttcggt gcggtcgatg gcgaaggcgg gtatgaggac cgagccgccc
    16621 cgctggaccg tgcgccggat gacgtcggcg agggcttcgt ggggcaggtt gaccggttcg
    16681 gggtgctcgc ggtcgccata ggtcgactcg atgaccacca ccggtgagcc cggcgggatg
    16741 tcccgggggc gcagcaccgg gtggtcgtgg cgccccaggt cacccgagaa gaggaccgac
    16801 ggcgccggca taccgtcggg tgccggcatc cggaccgtga ccgacgacga gccgaggatg
    16861 tggccggcgc gggtgaagcg cacccagatg tcgtcgccga ggtcggcgtc ggcgtcgaag
    16921 tcgacgggcg tcagcaatgg cagggtgcgc tcgacgtcgg cgggggtgta gatcggcagc
    16981 ggcgggttgt gcttcgacca gccgccctgg gccgcgtccc gagcctcgcg ttcggcgaga
    17041 aaacccgcat cccgcaacac gatcgccgcc aggtccttgg tgccggccgt gcaccagact
    17101 ggtccgtcga agccgtgttt gacgagggcg ggcagatagc cgcagtgatc caggtgggcg
    17161 tgggtgagga tgatgtcgct gatgctgtcg ggcggcacgg ggaagtcttc ccagttcttc
    17221 aggcgccact gcttctcgcc ctggaacatg cccgcgtcaa cgaggatgcg gcggccgccg
    17281 atctccaaga gatacttcga gcccgtaacg gtctgggcgg cgcccaggaa ggtcatggtt
    17341 gcgacaggca cggcgactcg ctcctcatcg atgacgttgc ctctccaccc taacgagccg
    17401 accgggacct ggagcgatgg actctggatc gcataccccc gtgggtataa agtgacgata
    17461 tggcaagtga tccgtcgacg atttccggcg agcagggcaa tacctgcggg ggtagcattg
    17521 cgccccacat cggcaacggt tcaggaaggc cattcatgga actcgacccc accgagatgg
    17581 atgcgatcct caagcgtctc aagcgcgccc aggggcagat cggcggcatc atcaagatga
    17641 tcgaggacgg gcgcgagtgt caggacatcg tcacccagat ggcggcggtg tccaaggcga
    17701 tcgaccgcgc cgggttcgcc gtcatcgccc tcggcctgcg ccagtgcatc gccgaccccg
    17761 aggggcatca gatggacgtc gcgtcgatgg agaaactgtt cctgtccctg gcgtgaggcg
    17821 ttggcggcat acatacccca tggggtatgc tgtgaccgag gcgagagggt gacctccagc
    17881 cgtcccaacc tcccccgaac tcaggagcag agaacacatg tgccgagcag tcgtgtgcaa
    17941 gaagtgcggc aagaccacgt gggccggctg tggtcagcac gtcgatcagg tcatgcgcgg
    18001 agtcccgaag gcccagcagt gccagggtca cgccgacgaa ccgagccagg gcgggttcct
    18061 cggcaagctc ttcggccgct gaatctccct gcgcgccaac tgcatcgcgc caacgccagc
    18121 aacctctagc ggtctctgga acccagcaga ggctagtcaa atctgcacac cgtgctaggt
    18181 gtgcagattt gactagcctc tgctagcaga ggttgctggg tctgggcccg ggctagtggt
    18241 cgcccttgag gaccatccgc atgatccgag tgttgaagac ggcgaagcgg gtgagctgaa
    18301 tcgggacgct cttgcgcttg cgcagggtcc cggccgtggg caggggcgcg cgcgacaggt
    18361 cggcgacggg gaccggtgtc tccgccgggg tggatgtcat atcggtcatg tcgattactc
    18421 cgggatgaac tgccagccga gccggccctt ggccttgtgg atgaagaggt agtgcagcat
    18481 cagcttgacc cagtggcccg agagcccgag ttcgccgcgc gtgtccttca ggtcgcgacc
    18541 ggtggcatag cggtcgtagt cgggcacgat gggcatcatc gtcatcgcgg cggccgaccc
    18601 ggtgcgcagg cccgcgccgg cggaggcgat gcaggcggcg cccatattgg ccatagaggc
    18661 ctcatgcgcc ttggcccccg gcccgtgctt gatgcggtcg acgatcgaga gggcggcggt
    18721 cttgcccatg acacccgacg gcatcccggt gcgcggcggt gagggcgcga tcatcgtgcc
    18781 gttcggcgtc ttgcgcggct tggagatctg gtggggcggg gcgaaggcga tgccgacggc
    18841 ccagatgttg tcgtagccct gggcgagata ggtcttcggc cagtcgctcg ccttccactg
    18901 ctcatacggc ttgccgctgt agtcggcgtc gaccttcatg aagccgctcg gcgcgaagag
    18961 ttcgctcgtg atgtcgctgc cgtcacggtc gtaggcggcg agcggcacgc cgccgaaggg
    19021 cggcagcagc atcgcgaaat cgaatgcgag agagtgcatc tgcccgtcga gcgtctcgaa
    19081 gtgcacgacg cccggctcga ccgtggtcac gtgcacgccg aggaccgcct cgacaccgcg
    19141 ctcgcggaag agcgatccgg tccacagctc ggagctggtc tcgaagccca tgtcgtcgaa
    19201 ggtcatgccc ccgaccccga agtcgccgag ctcggcctca ttggtcagat agacgacgcg
    19261 cgccagatcc cgcacgcctg cctcgcgcag ttcgtggtcg acgttgaaga catactcgaa
    19321 tgccgcgccc tcgcacgtgc acgtgccgtg gcccatgccg acgacgaggg tttgccgctg
    19381 accggacttg agtttggcga tgcttgcctc gagggcagcg gccgcctcga tcgcgtgatc
    19441 ggccgtgcag accgagacgg tgtgaccacc atgcggtccg aggccctcgg tcatctggaa
    19501 tttcagttgc ggcccagtgg cattgacgag gtagtcgtag cggatacgtt ccgtctcgcc
    19561 gtcccgggcg gggctcgtgt gcacgatgtc gacggcaggc cgctgactcc cggcgtcgcc
    19621 gcgcgggtgg atcgcgaccg ccttggcctg ccggaactgc acccccttct tcgcataaac
    19681 cggcgcgagc gggaagacga cgtccttctt ggacatcttc cccacaccga cccagatatt
    19741 gctcgggatc cagttccact gcgagttggg cgacaccacg gtgacggtgt gacccttggg
    19801 cagcagccgg ctcaggtgaa gggcggcggt gtgccccgaa acccctgcgc cgaggatgac
    19861 gatgtcggcc atgcctactt ctcctctgaa tggcggctgt ctcgcagcgt acccctaggg
    19921 gtataaagta agcaaacccc caggggtatg tcttgctcga acgtaaggtg acttcctcac
    19981 gcgtgggtga gcaccgtctc ggtcagcacg cagaggccca tggccaggac gaaccagccg
    20041 aacccgcgtc gcaggtgcgc agggtggacc cggccggtca gccgcccgcc gacgacagag
    20101 ccgaccacgg cggcagcggt cacggcgagc accgtcgccc acggcaccga gaccgacgcc
    20161 aggtgaccgg cgagcccggc gaagctgttc atggcgatga cgaggagcga ggtgccgatg
    20221 gcctgacgca tcgggagccg ggcgaacaac gtcagggcgg ggacgatgag gaagccgccg
    20281 ccggcgccga cgaaaccggt gagcaagccc acgccgacac ccgcgagcag gaccggcccg
    20341 acggccagcg cgccggatgc cgcctcgccc gtctcgtcgc ggcggccgcg gatcatggcg
    20401 atcgccgtcg cgagcatgag gagggcgaag gtcgccagca acacccattc cggaacgtat
    20461 gccgcgagcc ggcccccggc atacgcaccg gccatcgcag cgactcccag gacggcgccg
    20521 gcgcgccact gcacccggcc gccgcgagcg tgcgggagga ccgcggcgat cgccgtcacg
    20581 ccgatgacga agagggaggc ggcgatcgcc gtgccgggag cgagccgggc gacgtagacg
    20641 agcagcggca ccatgaggat cgatccgccg ccgcccatca gccccagggt gacgccgacg
    20701 agcagcgcca gggtcagggt gagcaggagc atcgggacgc ggtcaggccg tcgtggtcgc
    20761 gcagctcgcc ggctcgaggt cgtgcgccga ccgccacgcg ttgtaaccgc cgaggatgtc
    20821 gctgacgtcg gcaaaaccct tggcgcgcaa caggctcgag ccgacgctcg agcgccagcc
    20881 gccggcgcag taggccagca ccggacgttc cttgtcgagt tcggcggcgc gccggccgag
    20941 ttcggcgagc ggaatgtggc tggcgccggg gatcatcccg ccggccacct cgccggggtt
    21001 gcggatatcg agcagttgca gccccggcac cgactcgagt gccgcgtcgg cctcggccac
    21061 gctcagccgg ctcgccaccg ccatgtcgtc gggatgcgcc aggaagtacg cctctggctg
    21121 cgggatgaac ccgaggacgt tgtcgtagcc gatccggtgg aagcgggtgg cgacctcctg
    21181 ctcctgcccg tcgggcgcga tgaggatcac gcgatcctgg gggccgagga ccatgcccac
    21241 cgtctccgcg agccgcccgt ccgaggggac gttgagcgac ccgcggaggt gggcgacggc
    21301 atactcggcg ttgggtcgag cgtcggtgac gatcgcgccg ccggcgagcg cgtcgcgaat
    21361 ctcgttctcg gacaatgcag aaacgtggtg gtcgacatcc aggatcgagc gctcctgccg
    21421 attgaccgtc gcgttgtaga cgaagtatcc cggggcgctg ggctgcccct cggtcacgac
    21481 gtcgaggaac tgctcacgcg tcatcggctg gcacgcgtag ttcgtcgcgc gctgagcgcc
    21541 gatcgtggac tgcttctcgg tcgagagatt cttgccgcac gcggacccgg cgccgtgcgc
    21601 ggggaagacg cgcaccgggt cgggcaggcc catcagtttg gtgtggatcg agtcatagag
    21661 catcgagccg agttcgtcgg cggtgaagcc gatggaggcg agcaggtccg gccggccgac
    21721 gtcgccgatg aacagcgcgt caccggtgag gacggcgtac gccacctcgt cgtccgcgtg
    21781 ctcgtagacg agcaccgaga tcgactcggg ggtatggccg ggcgtgtgca tgatctcgag
    21841 cgtcacgtcg ccgagggaga gccgctcgcc gtcggcgaag gcgcggatgt cgaagtcggc
    21901 tttggcctgg tcgccgtagc cgatccaggc gccggtggcc ttggccagct cgaggtggcc
    21961 ggagacgaag tcggcgtgga agtgggtgtt gaggatgccg acgatcgtca ggtcggcttc
    22021 ctttgcgtca gcgaggtagt cgtcgatgtc gcggcgcggg tcgacgacca cggccaggcg
    22081 ggtggtctcg tccgcgacga ggtaggaggc ctgcgagagg cagtcgaggt agtactgggt
    22141 cagaatcatg gtggtgccct ctctcgtgtc gtgggaggcg tctggtacat accctagggg
    22201 gtatattgac gagagacaac tccacccgtc agaaagccat tcccatgacg cgtcacctga
    22261 cctccaccgc tgccgccctg acgctcgcga tcgccgccct cgccggctgc tcgggcaacg
    22321 acccggcgcc ctccgcttcg gcagcatccc cctccgcctc ctcggcgtcg ggcgcgggag
    22381 ccgcgtccgc gactgccgcc ccggccaacg gggcgaccct cgacgccgcg gccttcgccg
    22441 ccgccctgaa ggcaccgcat acgaccatcc tcgacgtgcg cacgccggcc gagttcgcct
    22501 ccggccacct ggaaggggcc gtcaatgccg acatcgaggg cgacttcgcg gcggccatcg
    22561 ccgatctcga caaggccgcg ccgtatgccg tctactgccg ttccggcaac cgctcggcga
    22621 tcgctgtgca ggcgatgcag caggccggct tcacggcgac ctatcacctc gacggcggaa
    22681 tcaccgcgtg gcaggcggcc ggtgggacgg tcgtcaccgg ctaacgtcgg aggcatgacc
    22741 gagttcgagc gcaagttcgt cgtcaccgat ccgggtccgg cgacggc
//
LOCUS       DAOPJG010000026        21966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2526_length_21966_cov_7.978778, whole genome shotgun sequence.
ACCESSION   DAOPJG010000026 DAOPJG010000000
VERSION     DAOPJG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 21966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21966
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2526_length_21966_cov_7.978778"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKU75_04230"
     CDS             complement(<1..432)
                     /locus_tag="PKU75_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF80116.1"
                     /translation="MTFQETERQSRSALTAAGLTFGYPNGATLLDRLNLNVLPSSLTC
                     VTGPSGAGKSTLLYCLAGVLPAQGHIEIMGHRLSPDPAQRAAIRMAHCGFIFQRGELL
                     PEMTVLENVALPLRLTGTGRHDAINAAQHWLDRLGIAECAPR"
     gene            complement(449..739)
                     /locus_tag="PKU75_04235"
     CDS             complement(449..739)
                     /locus_tag="PKU75_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80117.1"
                     /translation="MAALAGLAVSGGVAQAASVSTTGSKAWNSGTHLAVQDTKADGAN
                     AYANWNQSTGNRIETSGGYGSTNEVFLTTLTSFRACRNLSANPDNCSSWVSP"
     gene            2271..6215
                     /locus_tag="PKU75_04240"
     CDS             2271..6215
                     /locus_tag="PKU75_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPF80118.1"
                     /translation="MSVPSQIPTADPLRRGPLAPPPRTLVDILRTTADAWPDSPAIDN
                     GETVLTYAEFLTAAEEFALELAVHGVGLGDRVGIRITSGTADLYVAIAATLLAGAAYV
                     PVDADDPDERARTVFGEAGVAAIIGNALTITSTDPGRPPVAREEPGVEDDAWIIFTSG
                     STGKPKGVAVTHRNAAAFVDAESRMFLQDNPIGPDDRVMAGLSVAFDASCEEMWLAWR
                     YGACLVPAPRSLVRSGTDLGPWLIANQITVVSTVPTLVLLWPAAALDQVRLLILGGEA
                     CPPEIGLRFATPEREVWNTYGPTEATVVACGARVRPGEPVRIGLPLDGWDLAVVDPNT
                     GIPVPGGELGELIIGGVGLARYLDPAKDAEKYAPMPTLGWERAYRSGDLVRLDPDSPA
                     AGLLFQGRADDQVKVGGRRIELGEIDATLTALPGVKGAAAAVRKTKTGNSLLVGYVAV
                     DDTFDRAAAIAILRRDQPAALVPRLAVVDDLPTRTSGKIDRDALPWPVHETVEQRSEP
                     SRSVTEQWIAELWRDVLGADDVSADADFFDLGGGSLSAAQAVGLIRARHPGFTVAEIY
                     EHPTLAGLAAYLDELEGPTGPRNRPTERVPRKTQIAQVLATVGLRTIAGLRWLTLVAT
                     ASTLAHRSGMPIANVASWWWLALGWLVLITPPGRMLLAAGGIRVILRGVGPGNYPRGG
                     RTHLRIWLAERWAAECGAVRISGAPLLPWYARLLGARIGTGVDLHTAPPVTGLLRIGD
                     GASVEPEVDLMTHWLDGDRLFVGSIRLGPRCRIGARTTIAPDTYVHAEAEIAAGSGVV
                     GEVGRGEYWAGSPSRKIAPARGPWESRPAPIRPSWRVAYAATAVLLDILPFLAGLVGF
                     GVLWPHVRAADSGSAAARAALVWLAPATLAAAATLVAAVWILVRLLGIGLAEGHHPIH
                     SRPAWQAWTTFRILDEARWWLYPLYASSLTPAWFRSLGASIGADAEVSTAVLIPKLTA
                     VGEGAFLADDTLIGGYELGGGWLRVAPVKIGKRAFVGNSGMAAPGRKVPKASLVAVLS
                     AAPRRGRAKAGTSWVGSPPERLRRQGDEVDAARTHRPALRLRILRGAVEVCRLLAVLA
                     HTALLVLVTVALLALAGRGWWLAAVLAGVVFIVAGLVAAAVAVLAKWLLVGRVRPGEH
                     PLWSSFVWRNELADAFVESLAAPWFAEPATGTTVLVHWLRAMGARIGHGAWIDTYWLP
                     EPDLIDLREGATVNAGCVVQTHLFHDRLLRMDTVTLDRGATLGPNSVVLPAAVIGRHA
                     TIGPASLVMRGEVVPDRTRWIGNPIGPWLEPTVRQDRAFDPHDTNRDDPDEE"
     gene            6218..7531
                     /locus_tag="PKU75_04245"
     CDS             6218..7531
                     /locus_tag="PKU75_04245"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPF80119.1"
                     /translation="MPRTGTDTYVPGHGDRRWSATAYTLELDYKVATNHLSGWAHVDV
                     QLAEAGRDIELDLYGLRVSSVLIDGQKAKNYSHNGSRLKVTLRPDGGDRIRLSVAYAG
                     KPRPMPGPDGRAGWEELTDGVLVGSQPHGAPSWFPCNDRADDKAPYRIAMSVESGYHL
                     VGNGALVDKRTRSGRTTWTYEQREPMAPYLACVQIGRYAVTHLAAQPVPVDVVAPAGT
                     RIGAGSAFEHQGAMVTAMSAWFGPYPFGAYRAVVAADTFEIPLEAQGLASFGINHVTP
                     AWENERLVAHELAHSWFGNSLTARSWADIWLHEGFACYSEWLWAEHRGMRSVEDQARE
                     QWKRLSRLPQDLVIGAPGADRMFDDRVYKRGALTVHALRLALGDESFFAAVRAWTAAH
                     AYGVVTTPDLIAHFGSVEAPADRAVPQVDSIIQTWVYAAPLPELG"
     gene            complement(7485..7991)
                     /locus_tag="PKU75_04250"
     CDS             complement(7485..7991)
                     /locus_tag="PKU75_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80120.1"
                     /translation="MRIVVLGATPQGSGVALVRDALASPVARVTRLCPRCGSSRHGPV
                     IATGVTPRLWVSVSHSPQATVVVLDTAGPVGVDIEPLDRADAAVLGQWTATEAALKAT
                     GRGLADDPDSVRLLEGDWAGDCLLEVPGPATPYRVRVTRVDVAGHAARVAQLIPALAA
                     GRHTPRSG"
     gene            complement(8002..8676)
                     /locus_tag="PKU75_04255"
     CDS             complement(8002..8676)
                     /locus_tag="PKU75_04255"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HPF80121.1"
                     /translation="MTDPKQQLTTAEIDDSGLGDWRLVCNRLKARFRTGDFATGLALV
                     DRIGAAAEAADHHPDVTLTYPEVIVALSSHDVRGITSRDIELARKISEFAAEMGVSAD
                     VAGLTQLELGLDTARGDQLAGFYAALLGGEVKDGEPVDASGQVPTVWWQETDREHDEF
                     ALPAAGHEQRWHFDVWVPADDAERRVQAVLEAGGTLVSDAAAPAYWVVADADGNRSCI
                     CTPAGR"
     gene            complement(8741..8962)
                     /locus_tag="PKU75_04260"
                     /pseudo
     CDS             complement(8741..8962)
                     /locus_tag="PKU75_04260"
                     /inference="COORDINATES: protein motif:HMM:NF020839.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
     gene            complement(9026..9649)
                     /locus_tag="PKU75_04265"
     CDS             complement(9026..9649)
                     /locus_tag="PKU75_04265"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPF80122.1"
                     /translation="MARLLEVHPVDPQPRAIQQAVALLREGGLIAYPTDSGYALGATM
                     GNQEAKDRIHAIRRLDDKHHFTLVCKDFAQLGQFVFIDNSVFRAVKAATPGPYTFILP
                     GSKDVPRKLLHTKKKTVGVRIPDSRVVQALVTELGEPLMSSTLILPGESEPMTEGWLV
                     KDELDHAVDIVIDTGECGSEPTTVVDFSSDEPEVVRVGAGDPHRFAW"
     gene            complement(9681..9950)
                     /locus_tag="PKU75_04270"
     CDS             complement(9681..9950)
                     /locus_tag="PKU75_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPF80123.1"
                     /translation="MQIRVRVKPGASRTRVGGRYGEDHLVVAVSAPAVDGRANEAVCR
                     ALAAAFGLRPRQVTIVSGVTARNKLVSLDGDAEVLARRVTDLLEA"
     gene            9981..11147
                     /locus_tag="PKU75_04275"
     CDS             9981..11147
                     /locus_tag="PKU75_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPF80124.1"
                     /translation="MDCHYYDAGVCRSCTRMGMPYADQLRDKQSAAAAVLAAHVAPAA
                     WRDPFAGTESGFRNKAKLVTGGAPGEVTVGILDARGRGVDLRDCGLYEAPLQAAMTPV
                     VRIVEDLRLLPYDVPKRRGELKHLLLTTSPAGELMVRFVLRSTGQLARIRDALPRLLS
                     EVPGTRVVSANIQPEHKAVIEGADEIVLTDDDTLPMDLDGIRLHLRPNSFFQTNTSVT
                     KGLYRQAREWISDLDPTVVWDLYCGVGGFALNAAGPGRRVFGSEVSAEAIASARISAA
                     ERGGEVHFEAGDSARVLAESGWPAPDLVIVNPPRRGLDADLAAQIENGPAAYLLYSSC
                     NPQTLARDLDRMPSFQVRAARLFDMFPQTTHAEVLILAGRVPTTGQPRAATAAS"
     gene            complement(11117..11614)
                     /gene="tpx"
                     /locus_tag="PKU75_04280"
     CDS             complement(11117..11614)
                     /gene="tpx"
                     /locus_tag="PKU75_04280"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPF80125.1"
                     /translation="MAETAFKGNPVNTVGDLPAPGAAAPELNLTGTDLGDITLAGLAG
                     RRVVLNIFPSVDTGVCAASVRKFNELAAGLHNTTVVCASADLPFALGRFCGAEGIENV
                     VSGSSFRSDFGDDYGVTMTDGPLRGLLARSVVVVDTDGKVLHTELVPEITTEPDYDAA
                     VAALG"
     gene            11808..12791
                     /locus_tag="PKU75_04285"
     CDS             11808..12791
                     /locus_tag="PKU75_04285"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPF80126.1"
                     /translation="MQNPAAKQNSEANDSARSTAGHWASAFPIVEREDISAVTFSLTV
                     RHPLMAKAARPGQFVIVMSHAEGERIPLTIADFDAAAGTITLVIQAVGKSTREMQRLC
                     QVGTSLHGIAGPLGIPTPIEASTRSGAPPTIVCVGGGLGVAPVYPQARAWHDAGARVI
                     GVLGFRNRNLVFWENKFRDVCDELILCTDDGSAGTQGMVTDGLRLAIEAHPETSEVIA
                     IGPPVMMRACAEVTRPHGIRTSVSLNPIMVDGTGMCGGCRVRVGGEVKFACVDGPDFD
                     AHLVDFDDLASRLRRYGEEEAEAMAQWETVCRRAEHTQAASLASALPDARG"
     gene            12794..14299
                     /gene="gltA"
                     /locus_tag="PKU75_04290"
     CDS             12794..14299
                     /gene="gltA"
                     /locus_tag="PKU75_04290"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPF80127.1"
                     /translation="MAAKRTIRSIPQERTPVREQPPLERARNFVEVNLGYDASEAARE
                     AQRCLFCADQPCVRGCPVSIDIPGFIRQICDEDHQGAYDTIARTNLLPAVCGRVCPQE
                     NQCEGVCTVGESLEPVAIGRLERYVGDLAIANDWARRPYISPTAYRVGIVGSGPAGMA
                     CAADLAKAGCQVTVFEALHLPGGVLRYGIPDFRLPGEVIDAEVANLEKLGVRFECNTV
                     VGRLFTIEQMLEDLGFDAVFIGTGAGYPTMLGIPGDSLGGVLSANELLTRANLMQARD
                     FPVHDTPIPLGKQVAVVGAGNTAMDAMRVCLRLGAEQVHCIYRRSRTEAPARAEEVHH
                     AEQEGIEFHWLTNPVAVLDDGAGNVRAMRCVHMELGEPDDSGRRRPVPIPGSEFEFPC
                     DQVVFAIGTNANPIIGQTSKLTLNRWGYLQTDDDLATSIDGVFAGGDIVTGAATVIEA
                     MGAGRTAAAGIVRHLGIRDVRRRYDDGEERIFGIAAHERGHARVREVAALP"
     gene            14392..16206
                     /locus_tag="PKU75_04295"
     CDS             14392..16206
                     /locus_tag="PKU75_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPF80128.1"
                     /translation="MEIISDSGEGAQRCGQSFGAIAARAGNGVWTVEIIPAEIQPPAR
                     SVAGASGNRIRLGSQRITNGGDEADLVVAFNEQVLLGRVHMKELKPGCIILLEDMWRE
                     HRDPRIRQAYGETYDALEAAGYRLIEIPMERECRKLVADPRRGKNMFALGMLCAIYQF
                     DLGLAREQVATTFRKKSAALIESNTNLLVAGYTWARSNLDLAFHIPAVAAGGRQVVTN
                     GNTALALGVVASGMEICAMYPITPATSASHYLSEIFPKVGGIVHQAEDEIAAATFAIG
                     ASFVGKCAVTITSGPGYSLKQEALGLAVMGEIPLVVVNVQRGGPSTGQPTKVEQGDLL
                     TTIFGSHGDAPKVVIAPSTIEDCFYSVIAARRIAETFNVVVVVLSDASLATAQQPFAR
                     PEFDPAWLAPPIDQSQVPEAMQPYAWDATTGLSRRFIPGQPGGMYTLTGLAHDASAHV
                     AYEPAANEEGIRARSLKLAALQRSLKPPTIYGDDSGELLVVGWGSTKGAIEEAVDRAR
                     AEGRQVSALHLRFLNPLPPGLREIMERFDAVMTVEGNWSDRSDDSLIDESNRRYGALA
                     MLLRSRYLLDVDTWSEVGGQPLKPGRVHAAMLRKLDQR"
     gene            16203..17216
                     /locus_tag="PKU75_04300"
     CDS             16203..17216
                     /locus_tag="PKU75_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPF80129.1"
                     /translation="MTDLDHLDLTHASAAECLLRLYDERVEIEGFQKGVPRWCSGCGD
                     NAVLAALQRLLRDEGLRPEKTVFVSGIGCSSRLPHYMNTYGFHGIHGRALPVAEGVRI
                     ARPDLNVFVNSGDGDCFSIGAAHWIHAIRYNMKMVVMVHDNQIYGLTKKQASPTSPRG
                     TRSNTTPQGSFLEGLNPLTVTLGVQNVSFVAQAADWIPEVLYAILQAAFHHDGLSFIR
                     IVQRCPEWMPDNFEPWLHDPSRVQLLHHEGMPAFSSGLASLYPTRLDHDPSDIHRARE
                     LASQHDPIPIGVLFHDPTVPRYDELVRAETTHSPAAVRQLLDEEFDRFAVVSDGEGGG
                     VDD"
     gene            17209..19476
                     /locus_tag="PKU75_04305"
     CDS             17209..19476
                     /locus_tag="PKU75_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80130.1"
                     /translation="MTDTRHLAAAAQTPTTGVRNGQQNRASFYLTGHRMGTGLGDVDG
                     LGLRSAHFAAYGDLDRLRYDYPLVLTDDRDATNALQSLSGLVDALLAEMGDAPDSDRI
                     RHHAMALETEIRARSGHGPQRLSDVWAAAATSLVAAGGDEHLADSLARLTAALRIDGQ
                     LVACTADSSRQVVGHLWRLVQQERTDRLAARIERLRQQLGDILAAEQANSVAGLRPDR
                     LAQSLGPVVGAELDADALSRLLTGNRPATLLSDARRERVQRLLGVLDAQRFVPDEDDI
                     DGDGDLFEFVYTDPVAAVTAYQERFAATAELAKALVIAEMEADGRYREEVHDALFAKW
                     DVEDLESDILDLFPKYLVEIDAEAMTAEQSVVLLDALAAGIPVKVLVQVNDLLRGTSS
                     KERSPGGGVPARKLGRYVLAAGEAFVLQTASAGLFHVRERIVAGSRLPGAALFVVYSG
                     GGQWLGDLPPYLASAAATEARVFPAFCYDPSAGETWADRLSLGCNPQTDLDWPVHRVS
                     YADQQLQRVTLELPFTAADFWAGDARMQPHLAIAGPDRSEGTIAPFAEHFASNPGIWT
                     DELPYVLMVDRDDRLSRVFVDRPLVHRAQRDVEQWRRLQELGGVHNSHVARELARQRA
                     LFEAELDAAREAPAPAPAPGPVATTTPPTGEPGEPGAPDPDDHEPVARDPYLPWIETL
                     RCSTCNECTLINDRMFAYNENRQAFIADPDAGTYAELVQAAENCQVAIIHPAKPRNPA
                     EAGLEELVKRAEQFA"
     gene            19586..19783
                     /locus_tag="PKU75_04310"
     CDS             19586..19783
                     /locus_tag="PKU75_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80131.1"
                     /translation="MIATTAAATDATTAVTLRAEECTLLHGTGYAARAGSAHVTAPDR
                     VAVVAALSGVGAADLPTLVFV"
     gene            complement(19809..21749)
                     /locus_tag="PKU75_04315"
     CDS             complement(19809..21749)
                     /locus_tag="PKU75_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HPF80132.1"
                     /translation="MPGAPTAVHAAIDRLLSRLDAEHRDEFADRVDRWWPDVESGVTA
                     LYGQTHDIETLCVDLISRAARAFADRDPELHRLDRIRMLTPDWLQQPGMVGYACYTER
                     FADDLQGVATHLDYLGELGVSYVHLMPLLRPREGDSDGGYAVADYRSVRPDLGTTEDL
                     RNLARQMRAHGMSLVMDLVLNHVAREHVWAEKARAGEKEYRDYFYIYPDRTKPDEYEA
                     TLPEVFPDFAPGNFTWDEDVEGWVWTTFNEWQWDVNWSNPAVLGEYADIILFLANLGV
                     EVMRLDAIAFTWKRVGTNCQNQPEVHDLTQVLHALSRMAAPAVAFKAEAIVGPRDLIQ
                     YLGTGTHVGKVSDLAYHNSLMVQAWSMLAVSDTQLTRQALRALPSTPSTGTWITYVRC
                     HDDIGWAIDDRDAEAVGLNGFLHRRFLADWYAGDVAESWGRGMVFQFNEETGDKRTSG
                     TAASLAGLEAARNDDEIQHALARVFLIHALAYGWGGIPVIWSGDELGTLNDPIWAAEP
                     GHAEDNRWAHRPRLDWQAAERRGDLRTREGKIFQGLAHLAKVRASLPQLHASAESRIY
                     GEHIDRGLLATLRNHPSGPMLCIYNVTEDWRPFPGMEFEPIGFTNPVNALGNNHPVAI
                     GEDGYIWLSPFAAWWVVDQPVG"
BASE COUNT         3396 a         7765 c         7379 g         3426 t
ORIGIN      
        1 tcggggcgcg cactcagcga tgccgaggcg atcgagccag tgctgcgccg cgttgatagc
       61 gtcgtgccgg cccgtgcccg tcaaccgtag aggtagggcg acgttctcga gaaccgtcat
      121 ttccggcagg agttctccgc gctgaaaaat gaagccgcag tgagccatgc ggatcgcggc
      181 ccgctgcgcg ggatcagggc tgaggcgatg acccatgatc tcgatatgac cttgggcagg
      241 tagcacgccg gcaaggcaat acaacagtgt ggattttcca gctccggagg gtcctgtcac
      301 gcaggttaag gacgagggca gcacgttaag gttgaggcgg tctagcaggg tggccccatt
      361 ggggtaccca aacgtcagcc ccgccgccgt cagggcggag cgggactgac gctcggtttc
      421 ttggaaggtc acaggtgatc gagaagggtc aggggctgac ccagcttgag cagttatccg
      481 ggttagctga caagttccgg caagcccgga acgacgtcag agtggtcagg aagacttcgt
      541 tggtggagcc gtagccgccg ctggtttcga tccggttgcc ggtgctctgg ttccagtttg
      601 cgtacgcgtt agcgccatcg gccttggtgt cctggacggc aagatgggta ccgctgttcc
      661 acgccttcga accggtagtt gagacgctcg ccgcctgagc gacgccgcca gagaccgcaa
      721 gccctgcgag ggccgccacg ataatgcgtc gaccgttaag tctaaacatg ttccgttgca
      781 acgtttttcc cctactcttc gttgagttgg gctgactgac gcagccctgg tgtctggacc
      841 atacaaccat ccgggaggtc ccataaaggg aacgagtccc ggtcttcaaa gtgctatcgg
      901 cggccacgag aaactaccca ctgatggccg ggcggtctgt ccagtcctga gtgttcgtgt
      961 tcggcaccgg ccgatggaca cgaccctccc ggttggtctc gagcctatcc ttgatgtcgg
     1021 tgctgtccgg gctgggcgac cctcgggctc ggcgtggacg ccgctacgac ctggttccgt
     1081 tggttgctgc ggcggtcgcg gccactttgg ccggggcgtc gtcgttcgtg gcgatcgcgt
     1141 cctggttggc cgaccaggac gccgccacgg ctaccgcgtt ggggttctgc cagcgtgcgt
     1201 cggacgccgg aggcctcggt gttccggcgc ctgttccaac ggttggaccc agcgtccctg
     1261 gatgcggtca tctcggtgtg gatgtggacc acgaccgtca cggtgggcgg tcgccgagtt
     1321 agcgcgttgg acggccagac catggctgtc gtcgccgctg cccggatggt cttgggcgcg
     1381 gcggtgacca gctctatcat cttggcgctc caggcgtgcg caagctgagg gtcgtggaac
     1441 aagtcgagcg ggtcagcgtc gctgttggta ttgctgcgac gcgacgggtg gacgcgctgg
     1501 aggaccttct gcgggtcgac gagtggaaga tcgggccccc acatgggcac actcgcgcta
     1561 atccagcgca cgcggatgcg tgaccctgct ttcacgccgt tggacgccaa atcatgcttt
     1621 acggcccctg cgggggcggc acctaacgtc cggtagcgct ccggtagcgc gtcgaagtcg
     1681 ctgacttgga ctgtgacatc gtgcggtaaa ggtcgcgcga actcgagccg gacgaggccc
     1741 ccccgtcagc agcattgcgg cgatgagaag ggggccagaa cgtatggctg acttatgggt
     1801 cgtatcttcc gggttgagga tgcggactgt cgggagggat tgcacggtca cggtgtcgcc
     1861 ggtcccggga aagacgacag gtgtgtcgcg ggtgatgacg ccgagttcgc agttgccggt
     1921 ttgcggaaga cgcccaagga gagcggccgc agtgaggttt gagctgcccg tcaggctcca
     1981 tctggatgcg tcttcgcagc gggtgtgaag gggcgcacct tcccgaggaa gatctgaagt
     2041 ccaggtagct ctgatcagga ccggcgttga ccggcaatcc cactgagtaa tagtctactt
     2101 gacataatgt cgcttatcgg cggttaagaa cgggaggtgc atctaatgca aagactcccg
     2161 gatgatgctc aggtcgcggc aattgcgtgc gttcaagcta tggcgaactc ggcgagacac
     2221 gcgagaacct gcggcgggaa ggcgcactgg tccgggagac tgatcccacc atgtccgtgc
     2281 cgagccagat cccgacggcc gacccgctgc gccgcgggcc tcttgcgccg cctccacgca
     2341 ccctcgtcga catcctgcgg accaccgctg acgcatggcc agactcccct gccatcgaca
     2401 atggcgagac ggtgctgaca tacgctgaat tcctcaccgc cgcagaggag ttcgcactcg
     2461 agctcgccgt ccacggtgtc gggctcggcg accgggtggg gatccggatc acgtcgggca
     2521 ccgccgatct ctatgtcgcc atcgccgcca cgctgctggc cggcgcggct tatgtgccgg
     2581 tcgacgcaga cgacccggac gagcgcgcac gaaccgtgtt cggcgaggcc ggcgttgccg
     2641 ccatcatcgg caacgcgctg acgatcacgt ccaccgaccc cgggcggcca cccgttgcgc
     2701 gggaggagcc aggtgtcgag gacgacgcgt ggatcatctt cacctccggc tccaccggca
     2761 agcccaaggg cgtcgccgtc acgcaccgca acgccgccgc gttcgtcgac gccgagtccc
     2821 gaatgttcct gcaggacaac ccgatcggcc ccgacgaccg cgtcatggcc ggcctctcgg
     2881 tcgccttcga cgcgtcctgc gaagagatgt ggctcgcgtg gcgctacggc gcgtgtctgg
     2941 tgcccgcgcc ccgttcgctg gtccgcagtg gcaccgatct cggcccgtgg ctcatcgcca
     3001 accagatcac cgtcgtctcc accgtcccga ccttggtcct gctctggccc gccgcggccc
     3061 tggaccaggt gcgcctgctc atcctcggcg gcgaagcctg cccgccggag atcggactgc
     3121 gtttcgccac ccccgaacgg gaggtctgga acacctacgg ccctaccgaa gccaccgtcg
     3181 tcgcctgcgg cgcccgggtc cgccccggcg agcccgtccg catcgggctg cccctcgacg
     3241 gctgggatct cgccgtcgtc gacccgaaca ccggcatacc cgtgccgggt ggcgaactcg
     3301 gcgaactgat catcggcggc gtcggcttgg cccgctatct cgacccggcc aaggacgccg
     3361 agaaatacgc cccgatgccc accctcggat gggaacgtgc ctaccgcagc ggggacctcg
     3421 tccgcctcga ccccgactcc cccgccgccg gactgctctt ccaaggccgc gcggacgacc
     3481 aggtcaaggt cggcgggcgc cgcatcgaac tgggcgagat cgacgccacc ttgaccgcgc
     3541 tccccggtgt caaaggggcc gccgccgcgg tgcgcaagac caagaccggc aacagtctcc
     3601 tcgtcggcta tgtcgccgtc gacgacacct tcgaccgggc cgccgccatc gcgatcctgc
     3661 gccgcgacca gcccgccgcg cttgtcccac ggctggccgt cgtcgacgac ctgccgaccc
     3721 gcacctccgg caagatcgac cgtgacgcac tcccctggcc cgtgcacgaa accgtcgaac
     3781 agcgttccga accaagccga tccgtcaccg aacagtggat cgccgaactc tggcgcgacg
     3841 tcctcggcgc ggacgacgtg agcgccgacg ccgacttctt cgacctgggc ggcgggtcgc
     3901 tcagcgccgc ccaggccgtg ggcctgatcc gtgcccggca ccccggattc acggtcgccg
     3961 agatctacga gcatcccacc ctcgccggac tggcggcata cctcgatgaa ctcgagggcc
     4021 ccaccggccc gcgcaacagg cccaccgaaa gggtcccgcg caagacccag atcgcgcagg
     4081 tgctcgcgac cgtcgggttg cggactatcg ccgggttgcg ctggctcacc ctcgtcgcca
     4141 ccgcctcgac cctcgcccac cgatccggga tgccgatcgc caatgtcgcc tcctggtggt
     4201 ggctcgccct cggctggctc gtgctcatca ctcccccggg ccgcatgctg ctcgcggccg
     4261 gcggcatccg ggtgatcctg cggggcgtcg gacctggcaa ctacccccgc gggggccgga
     4321 cccacctgcg gatctggctg gcggaacgct gggcggccga atgcggcgcc gtgcgcatct
     4381 ccggagcccc gttgctcccg tggtatgccc gccttctcgg cgcccgcatc ggcaccgggg
     4441 tcgacctgca tactgctccc ccggtcaccg gcctgctgcg catcggcgac ggcgcgtccg
     4501 tcgaaccgga agtggacttg atgacccact ggctcgacgg cgaccggctc ttcgtcggat
     4561 cgatccgcct gggtcctcgc tgccggatcg gtgcccgcac caccatcgcg cccgacacct
     4621 acgtgcacgc ggaagccgag atcgcggccg gctcgggcgt cgtcggcgag gtcggccgtg
     4681 gcgaatactg ggccggttca ccgagccgaa agatcgcccc ggcccgcggg ccgtgggaaa
     4741 gccggcccgc ccccatcagg ccgagttggc gcgtcgcgta tgccgccacc gccgtgctcc
     4801 tcgacatcct ccccttcctc gccggcctgg tcgggttcgg tgtgctgtgg ccccacgtgc
     4861 gggcggccga ctccggtagc gcggcggcac gggccgctct ggtctggctc gcccccgcga
     4921 ccctcgctgc tgcagccacc ctcgtcgcag ccgtctggat cctcgtgcgg ctgctcggga
     4981 tcggtcttgc cgagggccac cacccgatcc attcacgccc ggcctggcag gcgtggacga
     5041 ccttccggat cctcgacgag gcccggtggt ggctctaccc gctgtatgcc agcagcctca
     5101 ccccggcctg gtttcgctcc ctcggcgcga gcatcggcgc ggacgccgaa gtctccacag
     5161 ccgtcctgat cccgaagctc actgccgtcg gcgagggtgc cttcctcgcc gacgacaccc
     5221 tgatcggcgg ctacgaactc ggcggcggct ggctgcgggt cgcgccggtc aagatcggca
     5281 aacgcgcctt cgtgggcaac tcggggatgg ctgccccggg ccgcaaggtg cccaaggcga
     5341 gcctcgtcgc ggtgttgtcc gccgcgccgc gacgtggacg cgccaaggcc ggcacctcgt
     5401 gggtcgggag tccgccggag cggctgcgcc gccaaggcga cgaggtggac gccgcccgca
     5461 cccaccggcc cgcgttgcgg ctgcggatcc tgcgcggcgc cgtcgaggta tgccgcctgc
     5521 tcgccgtcct cgcgcacacc gcactcctcg tcctggtcac cgtcgcgttg ctcgccctcg
     5581 ccggccgcgg gtggtggctg gcagcggtcc tggctggggt ggtgttcatc gtcgcggggc
     5641 tggtggccgc cgcggtcgcc gtgctcgcca agtggctgct ggtgggccgt gtgcgcccgg
     5701 gcgagcaccc gctgtggagc tcgttcgtgt ggcgcaacga actcgccgac gcattcgtcg
     5761 aaagcctcgc cgcgccatgg tttgccgaac cggccaccgg gacaacagtt ctcgtgcact
     5821 ggttgcgggc catgggcgcc cggatcgggc acggcgcctg gatcgacacc tactggctgc
     5881 ccgagcccga tctgatcgac ctgagggaag gggcgaccgt caacgccggc tgcgtggtgc
     5941 agacccacct cttccacgac cggttgctgc gcatggacac cgtcactctc gaccgcggcg
     6001 ccacgctcgg ccccaacagt gtcgtcctgc cggccgccgt catagggcgc cacgccacga
     6061 tcggtccggc gtccctcgtg atgcgcggcg aggtcgtgcc cgaccgcacg cgctggatcg
     6121 gcaaccccat cggcccctgg ttggagccga ccgtcaggca agatagggcg ttcgaccccc
     6181 acgacaccaa ccgcgacgac cccgacgagg agtgacggtg ccacgcaccg gaaccgatac
     6241 ctatgtgccc ggccacggcg accgccgctg gtccgccacc gcctatacgc tcgagctgga
     6301 ctacaaggtg gcgaccaatc acctgtccgg atgggcgcat gtcgacgtcc agctcgccga
     6361 ggccggccgg gacatcgaac ttgacctcta cgggctccgc gtctcttcgg tcctgatcga
     6421 cggccagaag gccaagaact actcccacaa tggttctcgg ctcaaggtga ccctgcggcc
     6481 cgacgggggc gaccgcatac gactctcggt cgcctatgcc ggcaagcccc ggccgatgcc
     6541 cggccccgac gggcgcgcgg gctgggagga gttgaccgac ggcgtgctcg tcggctccca
     6601 gccgcacggt gcgccgtcgt ggttcccgtg caacgaccgt gccgacgaca aggcgccgta
     6661 ccggatcgcg atgagtgtcg agagcggcta ccacctcgtc ggcaacggcg ccctggtcga
     6721 caagcggaca cgatcgggcc ggacgacatg gacgtatgag cagcgggagc cgatggcccc
     6781 gtatctcgcg tgcgtgcaga tcgggcggta tgccgtcact cacctggccg ctcaaccggt
     6841 gccggtcgac gtggtcgcgc cggcggggac gcggatcggg gccgggtccg cgttcgagca
     6901 tcagggcgcg atggtgacgg cgatgagcgc gtggttcggg ccctacccgt tcggcgccta
     6961 tcgtgccgtc gtcgccgctg acaccttcga aatcccgctc gaggcacagg gattggcgag
     7021 tttcgggatc aaccacgtca cgccggcctg ggagaacgaa cggctcgtcg cccacgaact
     7081 ggcgcactcg tggttcggca actctctgac cgcacggtcg tgggcggaca tctggctcca
     7141 cgagggattc gcctgttaca gcgagtggct ctgggcggag caccgcggga tgcgcagcgt
     7201 cgaggaccag gcacgcgaac agtggaagcg gctctcgcga ctcccccagg acctcgtcat
     7261 cggcgcgccg ggagcggacc ggatgttcga cgaccgcgtc tacaagcgcg gcgcgctgac
     7321 cgtccatgcc ctgcggctcg cgctcggaga cgagtcgttc ttcgccgccg tgcgcgcgtg
     7381 gacggcagcc catgcctacg gagtcgtcac gacacccgac ctgatcgccc acttcggctc
     7441 cgtggaggca ccggcggatc gtgctgtgcc gcaggtggat tcgatcatcc agacctgggt
     7501 gtatgccgcc ccgctgccag agctgggatg agctgggcca ctcgcgcggc gtggccggcc
     7561 acgtcgaccc gggtcacgcg cacgcgatac ggagtggccg ggcccggcac ctcgagcagg
     7621 caatcgccgg cccagtcccc ctcgagcagg cgcaccgagt ccgggtcgtc ggccaggccc
     7681 cggcccgtcg ccttgagcgc cgcctcggta gccgtccact gcccgagcac cgccgcgtcg
     7741 gcgcggtcga ggggctcgat gtcgacgccg acggggccgg cggtgtcgag gacgaccacg
     7801 gtggcctgcg ggctgtggct gaccgacacc cagagccgcg gagtgactcc cgttgcgatg
     7861 accggcccgt gacgcgaact cccgcagcgc gggcacaacc gggtgactcg cgccacgggc
     7921 gacgcgaggg cgtcccggac gagtgcgacc ccgctgccct gcggcgtcgc accgaggacg
     7981 acgatccgca ccggccgcgg ttcagcgacc ggcgggcgtg cagatgcagg agcggttgcc
     8041 gtcggcgtcg gcgaccaccc aataggccgg tgcggccgca tcgctgacga gtgtcccacc
     8101 ggcctccagg acggcctgga cccggcgctc ggcgtcgtca gcggggaccc acacgtcgaa
     8161 gtgccagcgt tgctcgtgac cggcggccgg caatgcgaac tcgtcgtgct cgcgatccgt
     8221 ctcctgccac cacaccgtcg gcacttggcc gctggcatcg accggttcgc cgtccttgac
     8281 ctcgccgccg agcagcgcgg catagaagcc cgccagctga tcgccgcggg cggtgtcgag
     8341 cccgagttcg agctgtgtca ggccggccac gtccgcggac acgcccatct cggcggcgaa
     8401 ctcgctgatt ttgcgggcca gctcgatatc gcggctcgtg atcccccgca cgtcatggct
     8461 ggagagggcg acgatgacct ccggataggt gagcgtgacg tccgggtgat ggtccgcagc
     8521 ctccgcggcg gccccgatcc ggtcgaccaa cgcgagtccc gtggcgaaat ccccggtccg
     8581 gaaccgcgcc ttgagccgat tgcacaccaa tcgccagtca cccaggcccg agtcgtcgat
     8641 ttcggccgtc gtgagctgct gcttcggatc cgtcatgcca cgacagtccc acatctgccg
     8701 gacgaattgc gtccggtccc gccgcgaatc ccccgatgcg tcaaccggct tcggtcgcgc
     8761 gcaggtatgc cggccacttg gccgcaccat cgggcacgag gtcggccaag gtgtcatccg
     8821 gattgaaggt cttgcagcgt tgcagcgaca ggcagccgca gccgatgcac gagtccaggc
     8881 cgtcgcgcag cgtctcgagg gccgcgatgc gctcctcgat ccgctcgccc cagccccggg
     8941 agatccgttg ccagtcggcc ttggtcggtc ccggccgagc ctacaagagc cggtccagaa
     9001 cgtgaagccg gattgaactt ccgcatcacc aggcgaagcg gtgcgggtcg ccggcgccca
     9061 cccgcaccac ctcaggctcg tccgaggaga agtcgaccac ggtcgtgggc tcgctgccgc
     9121 actcccccgt gtcgatgacg atgtcgaccg catggtccaa ctcgtccttg acgagccagc
     9181 cctcggtcat cggctcgctc tcgccgggca ggatcagcgt gctgctcatc aagggctcgc
     9241 cgagctcggt gacgagggcc tgcaccaccc ggctgtccgg gatgcgcacg ccgacggtct
     9301 tcttcttggt gtgcaacagt tttcgcggca cgtccttgct gccgggcagg atgaaggtat
     9361 acggcccggg cgttgccgcc ttgaccgccc ggaagaccga gttgtctatg aagacgaact
     9421 gtcccaactg ggcaaagtcc ttgcacacca atgtgaaatg gtgtttgtcg tccagcctgc
     9481 ggatcgcgtg gatgcgatcc ttggcctcct gattgcccat cgtcgccccg agggcatagc
     9541 cggagtcggt cggataggcg atcaacccgc cctcgcgaag cagcgcgacg gcctgctgga
     9601 tcgcgcgcgg ctgcggatcg acaggatgga cttcgagcag gcgggccatt cgccaactgt
     9661 atgccgggga cggcgccccg ctatgcctcg aggagatccg tgacgcggcg cgccaggacc
     9721 tcggcgtcac cgtcgagcga caccagcttg ttgcgcgccg tcactccaga gacgatcgtg
     9781 acctgacgcg ggcggagccc gaaagccgcg gcgagagcac ggcataccgc ttcgttggcc
     9841 cggccgtcga cagccggcgc cgacacggcg acgacgaggt ggtcctcgcc gtatcggccg
     9901 ccgacgcggg tgcgggaagc cccgggcttg acgcggacgc ggatctgcac acgccgattc
     9961 tcgcctaccc tgggcctgcc atggactgtc actactacga cgccggcgtg tgccgctcgt
    10021 gcacccgcat gggaatgccg tatgccgatc agctccgcga caagcagtcc gcggccgccg
    10081 ccgttctcgc cgcacatgtc gcaccggcgg cctggcgaga tcccttcgcc ggcaccgaat
    10141 ccggctttcg caacaaggcc aagctcgtca ccggcggcgc gcccggcgag gtgacggtcg
    10201 gcatcctcga cgcccgcggc cgaggtgtgg acctgcgcga ctgcgggctc tatgaagcgc
    10261 cgctgcaggc cgccatgact cctgtggtcc ggatcgtcga ggacctgcgg ctgctgccgt
    10321 atgacgtgcc caaacgccgc ggcgaactca agcacctcct gctcaccacc tcgcccgccg
    10381 gtgagctgat ggtccggttc gtgctccgca gcaccggcca gctcgcgcgg atccgggatg
    10441 cgttgccgcg tctcctcagt gaggtgcccg ggacgcgggt ggtgtcggcc aacatccagc
    10501 ccgaacacaa agcggtcatc gaaggcgccg acgagatcgt cctcaccgac gacgacaccc
    10561 tgcccatgga cctcgacggg atccgcctgc acctgcgacc caacagcttc ttccagacca
    10621 atacgtccgt cacgaagggt ctgtatcgcc aagcccggga atggatttcg gacctcgacc
    10681 cgactgtcgt gtgggatttg tattgcggcg tgggcggatt cgcgctcaac gcggccggac
    10741 cggggcggcg tgtcttcggc tcggaggtct ccgccgaggc gatcgcctcg gctcgcatca
    10801 gcgcggccga gcgcggaggc gaggtgcact tcgaagccgg tgactccgcc cgcgtgctgg
    10861 ccgaatccgg ctggccggca ccggatctcg tgatcgtgaa cccgcccagg cgcggcctcg
    10921 acgccgacct ggccgcgcag atcgagaacg ggcccgcggc atacctgctc tactcgagct
    10981 gcaacccgca gacgctggcg cgcgacctgg accggatgcc gagcttccag gtccgggccg
    11041 cgcgcctgtt cgacatgttc ccgcagacga cgcacgccga ggtgctgatc ctcgcggggc
    11101 gggtgccaac gaccggtcag ccgagggcgg cgaccgccgc gtcgtagtcg ggttcggtgg
    11161 tgatctcggg caccagctcg gtgtgcagca ccttgccgtc ggtgtcgacg acgacgaccg
    11221 agcgagcgag gaggccacgc agcggaccgt ccgtcatcgt cacgccgtag tcatcgccga
    11281 agtcgctgcg gaagctcgac ccggacacga cgttctcgat gccctcggcc ccgcagaagc
    11341 ggcccagcgc gaacggcaga tcggccgagg cgcacacgac ggtggtgttg tggaggccgg
    11401 cggcgagttc gttgaacttg cgcacgctgg cggcgcacac gccggtgtcg acgctcggga
    11461 agatgttcag cacgacccgg cgcccggcca gcccggccag ggtgatgtcg ccgaggtcgg
    11521 tgccggtgag gttcagttcg ggagcggcgg ctccgggcgc ggggaggtcg ccgacggtgt
    11581 tgacgggatt gcccttgaat gctgtttcag ccatggctca gtgtctagca cgtcggcccg
    11641 caccgaggac agggcgtctt gtcggtcgcg gtcgaagcgc cgtcgatcac ccctgcccag
    11701 gagtaggctg cacgggagat agcaagggcg agcccggtgt cggccaggcg gcctcgcttc
    11761 gggtgtcccc ccgcaccggc ccgcgcgatg tgacggacgg tgccaccatg cagaaccccg
    11821 cagcgaagca gaactccgaa gcgaacgact ccgcgcgttc gaccgccggt cactgggcca
    11881 gcgccttccc gatcgtcgag cgtgaggaca tctccgcggt caccttcagc ctgacggtgc
    11941 gccacccgct catggccaag gccgcccgtc ccggacagtt cgtcatcgtc atgtcgcacg
    12001 ccgagggtga gcgcatcccg ctgacgatcg ccgacttcga cgccgccgcc ggcacgatca
    12061 ccctcgtcat ccaggcggtc ggcaagtcaa cccgggagat gcaacgactc tgccaggtcg
    12121 gcaccagcct gcacggcatc gccggaccac tcggcatacc cacccctatc gaggcgagca
    12181 cccgttccgg tgccccaccg accatcgtct gtgtcggtgg cggtctcggg gtcgcgccgg
    12241 tctatccgca ggcacgtgcc tggcacgacg cgggcgcccg ggtcatcggc gtcctcggct
    12301 tccgcaaccg caatttggtg ttctgggaga acaagttccg cgacgtgtgc gacgagttga
    12361 tcctctgcac cgacgacggt tcggcgggca cccaggggat ggtgaccgac ggcctgcggc
    12421 tggcgatcga agcccacccg gagaccagcg aagtgatcgc catcgggccg ccggtgatga
    12481 tgcgagcgtg cgccgaagtg acccggccgc acggcatacg caccagcgtc agcctcaacc
    12541 cgatcatggt cgacggcacg gggatgtgcg gcggctgccg cgtgcgcgtg ggtggtgagg
    12601 tgaagttcgc gtgcgtcgac gggccggact tcgacgccca cctcgtcgac ttcgacgacc
    12661 tggcctcgcg gctgcgccgc tacggcgagg aggaggccga ggcgatggcg cagtgggaga
    12721 cggtatgccg ccgcgccgag cacacacagg ccgccagctt ggcctccgcc ctccccgacg
    12781 cgcggggctg accgtggccg cgaaacgcac catccgcagc atcccccagg agcgcactcc
    12841 ggtgcgcgag caaccgccgc tggaacgcgc gaggaacttc gtcgaggtca atctcgggta
    12901 tgacgcgtcg gaggccgccc gcgaggccca gcgttgtctc ttctgcgctg accagccgtg
    12961 tgtgcgcgga tgcccggtct cgatcgacat cccgggcttc atccggcaga tctgcgacga
    13021 ggaccaccag ggcgcctacg acacgatcgc ccggaccaac ctgctgccgg ccgtgtgcgg
    13081 acgcgtctgc ccgcaggaga accaatgcga aggggtctgc acggtcggtg agagcctcga
    13141 gccggtcgcg atcgggcggc tggagcggta tgtcggcgac ctggccattg cgaacgactg
    13201 ggcgcgccgc ccttacatct cccccacggc ataccgggtc ggcatcgtcg gctccgggcc
    13261 ggcgggcatg gcgtgcgcag ccgacctggc caaggcgggc tgccaggtga cggtcttcga
    13321 ggcgctgcac ctgcccgggg gcgtgctgcg ctacggcatc cccgacttcc ggctgcccgg
    13381 ggaggtcatc gacgccgagg tcgccaacct cgagaagctc ggggtgcgct tcgagtgcaa
    13441 caccgtcgtc ggacggctct tcaccatcga gcagatgctc gaggacctcg ggttcgacgc
    13501 cgtcttcatc ggcaccggtg ccggctaccc gacgatgctc ggcatccccg gcgactcgct
    13561 cggcggtgtg ctgtcggcga acgaactcct cacccgcgcc aacctgatgc aggcccgcga
    13621 cttcccggtc cacgacaccc cgattcccct cggcaagcag gtcgccgtcg tcggggccgg
    13681 caacaccgcc atggacgcga tgcgcgtctg tctgcgcctc ggcgccgagc aggtgcactg
    13741 catctatcgc cgttcgcgca ccgaggcacc cgcccgggcc gaagaggtgc atcacgcgga
    13801 gcaggagggc atcgagttcc actggctcac caacccggtc gccgtgctcg acgacggcgc
    13861 gggcaatgtc cgggcgatgc gttgcgtgca catggaactc ggcgagccgg acgactccgg
    13921 ccgccgccgg cccgtgccca tccccggcag tgagttcgaa ttcccttgcg accaagtcgt
    13981 tttcgcgatc ggcaccaacg ccaacccgat catcgggcag acctcgaagc tgacgctgaa
    14041 ccggtggggc tatctgcaga ccgacgacga cctggccacg tccatcgacg gcgtcttcgc
    14101 cggtggcgat atcgtcaccg gcgccgcgac cgtcatcgaa gcgatgggcg ccggccgcac
    14161 ggccgcggcc ggcatcgtgc gccatctcgg catccgcgac gtgcgccggc gctacgacga
    14221 cggcgaagaa cggatcttcg gcattgccgc gcacgagcgc ggccacgccc gcgtgcgaga
    14281 ggtggcggca ctcccatgac cctttccctc gacgatcacc tcaggcggga cggcgaccca
    14341 gcaaccggca gaccggcggc tgaggagata acggagatct cgcaacacgt cgtggagatc
    14401 atcagcgact ccggcgaggg cgcgcagcgg tgcgggcagt cgttcggggc catcgccgcg
    14461 cgcgcgggca atggcgtctg gacggtggag atcatccccg ccgagatcca gcccccggcc
    14521 cgcagcgtcg ccggcgcgag cggcaaccgc attcgactgg gatcgcagcg catcaccaac
    14581 ggcggcgacg aggccgacct ggtcgtcgcc ttcaacgagc aggtgctgct cggccgggtg
    14641 cacatgaagg aactcaagcc cggttgcatc atcctgctcg aagacatgtg gcgcgagcat
    14701 cgcgatccgc gcatccggca ggcatacggg gagacctatg acgcgctgga ggcggccggc
    14761 tatcgtctca tcgagatccc gatggaacgc gagtgccgca agctcgtcgc cgacccgcgg
    14821 cgcggcaaga acatgttcgc gctcggaatg ctctgtgcca tctatcaatt cgacctcggc
    14881 ctcgcacgcg agcaggtcgc aacgaccttc cgcaagaagt cggcggcgct gatcgagtcc
    14941 aacaccaacc tgctggtcgc cggctacacc tgggcccgct ccaacctcga cctggccttc
    15001 cacatcccgg ccgtcgcggc cggcgggcgc caggtcgtca ccaatggcaa cacggccctg
    15061 gccctcgggg tcgtcgcatc gggcatggag atctgcgcca tgtacccgat cacgccggcc
    15121 acctctgcct cgcactatct gagcgagatc ttccccaagg tcggcggcat cgtccaccag
    15181 gccgaggacg agatcgccgc cgccacgttc gccatcggcg cctccttcgt cggcaagtgc
    15241 gcggtgacga tcacctccgg ccccggatac tccctcaagc aggaggccct ggggttggcc
    15301 gtcatgggcg agatcccgct cgtcgtcgtc aacgtccaac gcggcggccc cagcaccgga
    15361 cagccgacga aggtcgagca gggcgacctg ttgacgacga tcttcggcag ccacggcgac
    15421 gccccgaagg tcgtcatcgc ccccagcacg atcgaggact gcttctattc ggtcatcgcg
    15481 gcccggcgca tcgcggagac attcaatgtc gtggtcgtcg tgctgtccga cgccagcctg
    15541 gccacggccc agcagccgtt cgcccgtccc gagttcgacc cggcctggct cgctcccccg
    15601 atcgatcaga gccaggtgcc ggaggcgatg cagccgtatg cctgggacgc caccaccggg
    15661 ctgtcgcgcc ggttcatccc gggccagccg ggcggcatgt acacgctcac cggactcgcg
    15721 cacgacgcgt ccgcacacgt cgcatacgaa ccagcggcca acgaggaggg catccgggcg
    15781 cgcagcctga aactcgctgc gctgcaacgg agcctgaagc ctccaacgat ctacggtgac
    15841 gactccggag agttgctcgt cgtcgggtgg ggctcgacca agggcgctat cgaggaagcc
    15901 gtcgaccggg cccgcgccga gggccggcag gtgtccgcgc tgcacctgcg cttcctcaac
    15961 ccgctgccac ccgggctgcg ggagatcatg gaacgcttcg acgcggtcat gaccgtcgag
    16021 ggcaactgga gtgaccggag cgacgactcc ctcatcgacg agtcgaaccg ccgctatggc
    16081 gcgctggcga tgctcctgcg ctcgcgctat ctcctcgacg tggacacctg gagcgaagtg
    16141 ggcgggcaac cgctcaaacc gggccgcgtg cacgccgcca tgctcaggaa gctggaccag
    16201 cgatgaccga tctcgaccac ctcgacctca cccacgccag cgccgcggaa tgcctgctac
    16261 gcctctacga cgagcgcgtc gagatcgagg gcttccagaa gggcgtgccg cggtggtgct
    16321 ccggatgcgg agacaatgcg gtcctcgccg cgttgcagcg cctgctgcgc gacgaagggc
    16381 tgcggccgga gaagacggtc ttcgtctccg gcatcggctg ctcgagccgg ttgccgcact
    16441 atatgaacac ctacggattc cacggcatcc acgggcgcgc gctgccggtc gccgagggcg
    16501 tgcggatcgc gcgcccggac ctgaacgtct tcgtcaattc cggcgacggc gactgcttca
    16561 gtatcggtgc ggcacactgg atccacgcca tccgttacaa catgaagatg gtcgtgatgg
    16621 tgcacgacaa ccagatctac gggctgacca agaagcaggc gtccccgacc tcaccacgcg
    16681 gcacccgcag caatacgacc ccgcaggggt ccttcctcga agggctcaat cccctgaccg
    16741 tcaccctcgg cgtccagaac gtctccttcg tggcccaggc cgccgactgg atccctgagg
    16801 tgctctacgc catcctgcaa gcggccttcc accacgacgg actctccttc atccgcatcg
    16861 tccagcgctg ccccgaatgg atgcccgaca acttcgagcc ctggctgcac gacccttccc
    16921 gagtgcagct gctgcatcac gagggcatgc cggcattctc gtcgggcctc gcctcgctct
    16981 atccgacccg cctcgatcac gacccgagcg atattcaccg ggccagagag cttgcctcgc
    17041 aacacgatcc gatcccgata ggggttctct tccacgatcc cacggtgccc cgctacgacg
    17101 aactcgttcg ggccgagacc acccactcgc ccgcggcggt gcgtcagctg ctcgatgagg
    17161 agttcgaccg gttcgccgtg gtcagcgacg gcgaaggtgg gggcgtcgat gactgacaca
    17221 cggcatctcg cggcagcggc ccaaacgccc accaccggag tgcgcaacgg ccagcagaac
    17281 cgcgcctcct tctatctgac cggacaccgg atggggaccg gtctcggtga cgtcgacggc
    17341 ttggggctgc ggtcggccca ctttgcggca tacggcgatc tcgaccggct ccgctacgac
    17401 tatccgctgg tgctgaccga cgaccgcgac gccaccaacg cgctgcagtc gctgtcggga
    17461 ctcgtcgacg ccctgctcgc cgagatgggc gacgcccccg actccgaccg gatccgccac
    17521 cacgccatgg cgttggagac cgagatccgg gcccggtccg gccacgggcc ccaacggctt
    17581 tcggacgtct gggccgcagc tgccacgtca ctcgtcgctg cgggcggcga cgagcacctt
    17641 gcggacagcc tggcccgcct gacggcagcc ctgcgcatcg acgggcaact cgtcgcctgc
    17701 actgcagaca gctcccgaca ggtcgtcggc cacctctggc ggctcgtgca gcaggagcgg
    17761 accgaccggc tggccgcgcg gatcgagcgg ctccgccagc agctcggcga catcctcgcc
    17821 gccgaacagg ccaactcggt ggcgggcctg cggcccgacc ggctcgccca gtccctgggg
    17881 cccgtggtcg gcgccgaact ggacgccgac gcgctgtcac ggctgctgac cggcaaccgg
    17941 ccggcgaccc tgctctcgga tgcgcgccgc gagcgggtcc aacggctcct gggggtgctc
    18001 gacgcccagc ggttcgtgcc ggacgaagac gacatcgacg gcgacgggga cctcttcgaa
    18061 ttcgtctaca ccgatccggt ggctgcggtc acggcatacc aggagcggtt tgctgccacc
    18121 gccgaactgg ccaaggcgct ggtcatcgcg gagatggagg ccgacggccg ctaccgcgag
    18181 gaggtgcacg acgcgctttt cgcgaagtgg gatgtcgagg acctcgagag cgacatcctc
    18241 gacctgttcc cgaaatatct cgtcgagatc gacgccgagg ccatgacggc cgagcagagc
    18301 gtggtcctcc tcgacgccct ggccgccggc atacccgtca aggttctcgt ccaggtcaac
    18361 gacctgctgc gcggcacctc ctccaaggag cgatcgcccg gcggcggcgt gccggcacgc
    18421 aagctcgggc gctacgtcct cgcagccggc gaggcgttcg tgctgcagac cgccagcgcg
    18481 ggcctgttcc acgtgcgcga gcggatcgtc gccggctctc gcctgccggg tgccgccctc
    18541 ttcgtcgtct attccggtgg agggcagtgg ctcggcgacc tgccgcccta tcttgcgtcg
    18601 gccgccgcca ccgaggcccg cgtcttcccc gccttctgtt acgacccgtc tgcgggcgag
    18661 acctgggcgg accgtctctc gctcggatgc aacccgcaga ccgatctcga ctggccggtg
    18721 catcgcgtga gctacgcgga ccagcagctg caacgcgtca cgctggaact cccgttcacg
    18781 gcagcggatt tctgggccgg cgacgcccgt atgcagccgc acctggccat cgccgggccg
    18841 gaccggtcgg aggggacgat cgcgcccttt gccgagcact tcgcgtccaa ccccggcatc
    18901 tggaccgacg aactgcccta tgtgctcatg gtcgaccgcg acgaccggct gagtagagtg
    18961 ttcgtggacc ggcccctggt gcaccgggcc cagcgcgacg tcgaacagtg gcggcgcctg
    19021 caggaactcg gcggcgtcca caactcccac gtcgcacgcg aactcgcccg ccaacgtgcc
    19081 ctttttgagg ccgagctcga tgcggcgcga gaggcaccgg cgccggcacc ggctcccggt
    19141 ccggtggcca cgaccacccc gcccaccgga gagccgggag agccgggagc gccggatccc
    19201 gacgatcacg aaccggtcgc ccgcgacccc tacctgccgt ggatcgagac cctgcgctgc
    19261 tcgacgtgca acgagtgcac gctgatcaac gacaggatgt tcgcctacaa cgagaaccgg
    19321 caggcattca tcgccgaccc cgacgccggc acctacgccg aactggtgca ggccgcggag
    19381 aactgtcagg tcgcgatcat ccacccggcc aagccccgca accccgcgga ggccggcttg
    19441 gaggagttgg tcaagcgcgc ggagcagttc gcgtaagcgg ctgatctctc tctcaggcaa
    19501 gttctggccg agtcacagcc cggtcacagg aaggcccgcg attctcagat ggacgcgaaa
    19561 aaccacctga gagaacggaa tccagatgat cgccacgact gccgccgcca ctgacgccac
    19621 caccgctgtc accctgcgcg ccgaggagtg cacgctgctg cacggcacgg ggtatgccgc
    19681 ccgggccggt tcggcgcacg tgaccgctcc cgaccgggtc gccgtcgtgg ctgcgctgtc
    19741 cggggtcggc gccgctgacc tgccgacgct cgtcttcgtc tgaccgggtc cgaacggatg
    19801 cgggtcggtc agcccaccgg ctgatcgacg acccaccacg cagcgaaggg cgacagccag
    19861 atgtagccgt cctcgccgat ggccaccggg tggttgttgc ccagggcgtt caccgggttg
    19921 gtgaagccga tcggctcgaa ctccatgccc gggaagggac gccagtcctc ggtgacgttg
    19981 tagatgcaca gcattggtcc ggaggggtga ttgcgcaggg tcgcgagcag ccctcgatcg
    20041 atgtgctcac cgtagatccg ggactcggcg gaggcgtgca actgcggcag ggaagcgcgg
    20101 accttggcca ggtgcgccag gccctggaag atcttcccct cccgggtgcg cagatcgccg
    20161 cgtcgctcgg cggcctgcca gtccagtcgc ggccggtgcg cccaccggtt gtcctcggcg
    20221 tgacctggtt ccgcggccca gatggggtcg ttgagcgtgc cgagttcgtc tccggaccag
    20281 atcacgggga tgccgcccca gccgtaggcg agcgcgtgga tcaggaagac gcgcgccagc
    20341 gcatgctgga tctcgtcgtc gttgcgggcc gcctccagac cggccaggga ggccgcggtg
    20401 ccactcgtgc gcttgtcgcc ggtctcttcg ttgaactgga agaccatgcc ccggccccac
    20461 gactcggcga cgtccccggc ataccagtcg gcgaggaagc gccggtgcag aaaaccgttg
    20521 agccccacgg cttccgcgtc gcggtcgtcg atcgcccagc cgatgtcgtc gtggcagcgg
    20581 acgtaggtga tccaggtgcc ggtgctgggt gtcgacggga gcgcccggag cgcctgtcgg
    20641 gtcagttggg tgtcgctgac cgcgagcatc gaccacgcct gcaccatgag ggagttgtgg
    20701 tatgccagat cgctcacctt gccgacgtgg gtgccggtgc cgaggtattg gatcaggtcg
    20761 cgagggccga cgatcgcctc ggccttgaag gccaccgccg gtgcggccat gcgcgagagg
    20821 gcgtggagga cctgcgtcag gtcgtgcacc tcgggctggt tctggcagtt ggtgcccacc
    20881 cgcttccacg tgaacgcgat ggcgtccagg cgcatgacct cgacgccgag gttggcgagg
    20941 aagaggatga tgtcggcgta ttcgccgagg accgcggggt tgctccagtt gacgtcccac
    21001 tgccattcgt tgaaggtggt ccacacccag ccctcgacgt cctcgtccca tgtgaagttg
    21061 ccgggggcga agtcggggaa gacctcgggc agggtcgctt cgtactcgtc cggtttggtg
    21121 cggtcggggt agatatagaa gtagtcgcga tactccttct cccccgcgcg ggccttctcc
    21181 gcccagacat gttcgcgtgc aacgtgattc agcacgagat ccatgacgag tgacatgccg
    21241 tgcgcgcgca tctggcgagc gaggttgcgc aggtcctcgg tggtgccgag gtcgggccgg
    21301 accgagcgat agtccgccac ggcatacccg ccgtcgctgt caccctcccg aggacgcagc
    21361 agcggcatca ggtggacata ggagacaccg agctctccga gatagtccaa gtgcgtggcg
    21421 accccttgca ggtcgtccgc gaaccgctct gtgtagcagg cgtatccgac catcccgggc
    21481 tgttggagcc agtcgggtgt gagcatcctg atgcggtcga gccggtgcag ctcggggtcg
    21541 cgatcggcga aggctcgcgc ggcacgcgag atcaggtcga cgcacaaggt ctcgatgtcg
    21601 tgggtctggc catagagggc cgtgacaccc gactcgacat ccggccacca ccggtccacg
    21661 cgatcggcga actcgtcacg atgctcggcg tcgaggcgag agaggagccg atcgatggcg
    21721 gcatgaacag cggtcggggc accgggcatg gcgcccattc tgacactcgc gagcagcttc
    21781 gccatggtgg tcagccaacc cccgccgatg tgtccctcat cgtcaccggc gtgccgatct
    21841 gcaccgtccg ggagacggtg cacagccggt cgtgcgacat ctcgatcgct ctcggcagca
    21901 tctcccgggc acggtcgccg gcctccccct cggggaactc gacggtgaag ctgatcgtga
    21961 tgtcct
//
LOCUS       DAOPJG010000027        21937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2530_length_21937_cov_7.726579, whole genome shotgun sequence.
ACCESSION   DAOPJG010000027 DAOPJG010000000
VERSION     DAOPJG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 21937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21937
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2530_length_21937_cov_7.726579"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1182)
                     /locus_tag="PKU75_04320"
     CDS             complement(61..1182)
                     /locus_tag="PKU75_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPF80133.1"
                     /translation="MVRSQQAARRITATVGALGLGLALAACGGGSDPTSSGAASGSGS
                     ATGGGSSGEKVGVALITKDSTNPFFVAMQKGAQADAAKNNVELTIASGKQEGDDQGQI
                     EAIEQAIARGDKGILITPMSEGVNAAIKNARDAGLYVIALDTPPDPADTVDITFATDN
                     RLAGQLIGKWAAAQLKGEKATIALLDLFNDKIVSVDYNRDQGFLEGMGIDVADGKKNG
                     DEAPTGKYSGGDYEIVCNEPGNGAEDGGRTAMEKCLAKNPNVNVVYTINEPTAVGANA
                     ALQAEGKKAMIVSIDGGCAGVQSVKDGVIGATAQQYPLKMATLGMEAIAKIARGGEKP
                     AVTEGLDFYNTGVALVTDTAADGVESITADEGAKICWGN"
     gene            1408..2403
                     /locus_tag="PKU75_04325"
     CDS             1408..2403
                     /locus_tag="PKU75_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF80134.1"
                     /translation="MRDVAAAAGVSLKTVSRVVNNEPGVTGAVRERVQAAAERLDYRH
                     NLAASNLRRSGSRTGVIGVLVQDVANSYSAGLLRALEDAMRVHDVMLVAGSLDETTAR
                     ERAMVTNLISRRVDGLVIVPASPRQDYLEPEIRAGLPIIFADRAPRGIDTDSVIVDNA
                     LGAYQAVDHLLARGHRRIAVLTDSASIATADERVRGARAAFAKHGLELPGDLLVRGHA
                     DQPGAAATVHRLRELPAPPTAIFAGRNAISKGTLQALSERGERESVAVVGFDDFPMAD
                     LLQVTVIRQDVAKLGALVAARLLERIAGDTTPPIRIVLPPTLIPRGSGEIPPPAA"
     gene            complement(2458..3084)
                     /locus_tag="PKU75_04330"
     CDS             complement(2458..3084)
                     /locus_tag="PKU75_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80135.1"
                     /translation="MSNYDPNNPQEQGGMPPPPPAYGNQGAYPQGQAPVPPAYGGMNA
                     MPGSGYTPPAPMARPSAMDTAVKLMQAGGVLSILSLLATFLMMDTLRDEVTKQMKEND
                     AGVSQSTIDAAVNVGLGVGILFGLLGAGLWFWMASANGKGKSWARIVATVFFVISLLS
                     FLFSFTQTQPLISRVLSVLSILIGAAAMAMMWKKESSAYYNSMSAPKY"
     gene            complement(3195..4172)
                     /gene="rlmB"
                     /locus_tag="PKU75_04335"
     CDS             complement(3195..4172)
                     /gene="rlmB"
                     /locus_tag="PKU75_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPF80136.1"
                     /translation="MPGNSSRRGATRKPGSKKGALVGSGGQRRKGLEGKGPTPKAADR
                     PNHKAHKAAQKAGAARPGTGTRPAGGSRSPQRKAKGGTEIVAGRNSVLEALRTDIPAS
                     TLYVASRIDSDDRVREVIKIAGERGIPMLETPRTELDRITDDAVHQGLALQVPAYSYA
                     HPGDLIDPEKPGVPVIVALDGITDPRNLGAIIRSVAAFGGHGVLVPSRRSVGMTASAW
                     KTSAGAAARIPVAQATNLTRALQDYRKQGFFVVGLDMDGDVDLPELALASEPLVIVVG
                     SEGKGLSRLVRETCDQIVSIPMTSAVESLNAGMAVGVTLYEIARSRRPA"
     gene            complement(4172..5611)
                     /gene="cysS"
                     /locus_tag="PKU75_04340"
     CDS             complement(4172..5611)
                     /gene="cysS"
                     /locus_tag="PKU75_04340"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPF80137.1"
                     /translation="MTLRLFDSAAREIRDFVPVTPGQVGIYICGLTTQAPPHIGHVRF
                     AVAFDVLRRWLMRGHGYDVTLIRNVTDIDDKILAKSAEAGEDWFALSYRNERLTSEAL
                     ELLGVLPASYEPRATGHVPEMIELMRVLIEKGHAYPAPDGSGDVYFDVRSWPAYGELT
                     RQKVEDMAAAEDADPRGKHDPRDFTLWKGSKPTEPESASWPTPYGVGRPGWHLECSAM
                     ARKYLGDTFDIHGGGVDLRFPHHENEQAQSRAAGMGFANYWLHNAWVTISGEKMSKSL
                     GNSLLIAEVTKHARPLAVRYYLTAAHYRSTIEFHAGSLAEAEAAVDRIEGFLRRALPG
                     EHTAQPTGTEILPEDFVAAMDDDLGVSGALAVVHEHVRTGNIALDEDEAEVAAQEARL
                     VIAMTDILGVNPLAPNWSGSDTAGQGEAADALDALVQAQLAARAGARAARDYATADAI
                     RDQLHAVGIQIDDTPTGARWSLVKHGGEN"
     gene            5789..7309
                     /locus_tag="PKU75_04345"
     CDS             5789..7309
                     /locus_tag="PKU75_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPF80138.1"
                     /translation="MSDVSRPSSLDAEQVGYKQTLGRRHVQMIAIGGAIGTGLFLGSA
                     SRLNSTGPALLFSYAFVGVIAYFLMRALGELVLHRATSGAFVSYMREFYGEFWAYLTG
                     WMYWINWALTGIAELSAVGLYIQHWWPDFATWKTVLISLAVVLIINLLSARAFGEFEF
                     WASVAKVAAILVFLAVGLVVVIGRMDIGDHRAGFSNLWSNPGGFWPHTDGFNWYGPIM
                     VMSGVVFAYAAIEMVAVAAGEMEDAEREVPKAVNAVIMRIAVFYCGSILLLVSMLPTS
                     EYKAGISPFVTVFGRMGLDWMGSVIQAVLIVAAMSSLNSGLYSTGRVLRSLGVAKQAP
                     AFTLKMSKSGVPWAGIVMTSIVYLFGAILNALTPHAFEIALEAAALGVLFTWGTIFAC
                     QLRLRKLVNKGVIPPSPFQAPGYPWTSYVGLAFLVYVVLAMAISGWQSSPYFWGKTNF
                     IVVVIGIPLIGALLLLGWMIVRPKVVANTGNRLGPVWSDDGPTYADARPVEPAQHP"
     gene            7328..8161
                     /locus_tag="PKU75_04350"
     CDS             7328..8161
                     /locus_tag="PKU75_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPF80139.1"
                     /translation="MNSWNRRTVTLLAGLAVVAGAGACDSGSSPTTSTTPTASGTGTI
                     KIGVSTDEPGVSLKEGNTYSGFDIKTAEYVAGKLGLTPQWVPIAQADRVSALQSGEVE
                     MVVATFSITDERKKQVDFAGPYFVAHQDLLIRLNDQTITRPERLDGKILCSVTGTTSA
                     AYVQSHYDKAKITLREVPSFSECVRNLANGDVDAVTTDDLILAGFAATPEYKGILKVL
                     GFGFTDETYGIGIKKGNTDLVTKVNGALKEYISSGAWKQALEETVAPSGYKIPNPPTV
                     G"
     gene            8161..10020
                     /locus_tag="PKU75_04355"
     CDS             8161..10020
                     /locus_tag="PKU75_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HPF80140.1"
                     /translation="MHDRDDLRAVLETHEPFSSASPEVRERVIASAEIAQYAAGTLVL
                     DAFSDPSSAVYLILDGQVGLWNDARKRPLEPDEILGPGRVFGFSAMITERSVGPKAIA
                     LTQARVARLPGEHAAAAFVTRQGARFLADNLLPHHEDTLPTRATGYQRLGDLIVESPL
                     QVPASASIAEVAKAMTDQGRTCAVVTLDDGFGLVTDASLRERVLVDGLPPTAPARDAL
                     DRTALHTPEDTSAAESLVGLIEADAEAIVVTTQDGRLAGVVTMREFALSPTAADLAVH
                     QQLRRAPTSADLTRHAQEIPSLLRHLLRRGLGSGRVIAVYSSLIDAIIHRAIEIELAK
                     RPDLPADGFTWYALGSNGRREAVLSSDVDSGVAFADGTSPELMSAYREVFAQVDVVLM
                     DAGFTGDAHGVSARHEILSRTDSEWLAAARGWLADPVADKGAIMTSLLVDSRPITGAQ
                     RRVAAEFVASELRSHPGTMRLLLLDALDRRAKWRSMLEGWRGPVRYDLKDHAILPLVN
                     LARWGALAVRSTALSTPDRLRACGGTSILPQAQANSLIEVFEVLQRLRLRYQLLQVAD
                     GARASDAVLIDQLSPIDRSVVAQAVREIATAQKRLANFSAYVDTDEWTRPAQG"
     gene            10026..10856
                     /locus_tag="PKU75_04360"
     CDS             10026..10856
                     /locus_tag="PKU75_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPF80141.1"
                     /translation="MTIERRYAVIGDVAGHYADLTQELRRLGADPEAGTLPPDLIVVQ
                     VGDLIHRGPQSDAVVALVDHFISTAPQQWIQLIGNHEAHYARAQVFEWHEQISARAIS
                     AMRAWWHEGIMQVATVVPSATGDLLVTHAGVTEPFWREDLGALPDPYAVAERLNQMGR
                     LGRKAVFRPGEMLTDRVAPRVGPLWASAGSELVASWVGHRLPFGQVHGHSSCYDWQRS
                     AWRPSVPSGAHIHLDHDAGHETVTLDGGVIIGVDPGHGARAHTAWRAWVSQGTSASRG
                     "
     gene            complement(11072..11611)
                     /locus_tag="PKU75_04365"
     CDS             complement(11072..11611)
                     /locus_tag="PKU75_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPF80142.1"
                     /translation="MRARILVAALAIGLTAPASGAPTAPPVVVPAEGTPAQLPVLDIT
                     AEVQTLVLPTANLDGSLTDFDGKRFRLDANVLFRFDESDITPKGEAALAELVGKLEAA
                     KATRVRIDGYTDDVGETAYNLRLSQRRADAVRDRLEAALGGKVTVTSAGHGEQNPVAS
                     NETKAGQALNRRVTVVVEK"
     gene            complement(11608..12267)
                     /locus_tag="PKU75_04370"
     CDS             complement(11608..12267)
                     /locus_tag="PKU75_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80143.1"
                     /translation="MAALGAIAGMSACTGSSDTATAPVTVTATDQGSAAGSAAADTAS
                     TGSAATGDVSVPQPASQSASATGPAAEPLPVIGTRKGAKDGNGNGMEVSLNSVTVNAD
                     LMTVTFSARSDSAESWMLGNYFYAGNPDEGKLDKTLEAGAGIVDGVYVLDAAAKKRYL
                     PARDKDGHCVCSSLGAQVIGAGSDVVLEAVFAAPPDSTTTVDVSIPNVGTFAKVPVTR
                     A"
     gene            complement(12443..13102)
                     /locus_tag="PKU75_04375"
     CDS             complement(12443..13102)
                     /locus_tag="PKU75_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF80144.1"
                     /translation="MSSEAGRAEVVATSPGGVRVERVITSGIFALDGGEWEVDNNVWI
                     VGDDAECFVIDAAHDAAAILAAVGDRSVRAVLCTHAHNDHITAAPAVREATGAPIALH
                     PADQVLWELTYPGTEPDEQLADGESLSIGDVELEVLHTPGHAPGAVCLYAASLGLVFT
                     GDTLFQGGPGATGRSYSDKPTILASIRDSLLVLPPETVVLTGHGDSSTIGAEATGSDF
                     A"
     gene            complement(13099..14181)
                     /locus_tag="PKU75_04380"
     CDS             complement(13099..14181)
                     /locus_tag="PKU75_04380"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HPF80145.1"
                     /translation="MPQTVKGVIARAKGAPVEVTDIVIPDPGPGEAVVQVQACGVCHT
                     DLHYREGGINDDFPFLLGHEAAGIVEAVGEGVTEVAAGDFVILNWRAVCGECRACHKG
                     KPQYCFDTHNAAQKMTLTDGTVLSPALGIGAFAEKTLVAAGQCTKVATSDPAVVGLLG
                     CGVMAGFGAAVNTGGVGLGDSIAVIGCGGVGNAAIAGAKVVGATTIIAVDVDDRKLQW
                     AKEFGATHTINSTSEDAVEKIRELTGSYGADVVVEAVGRPETYQQAFFARDLAGTVVL
                     VGVPTPDLKLELPLIEIFGRGGSLKSSWYGDCLPTRDFPTLVSLYQQGRFPLDAFVSE
                     RIGLGDIESAFNKMHKGEVLRSVVEL"
     gene            14329..14658
                     /locus_tag="PKU75_04385"
     CDS             14329..14658
                     /locus_tag="PKU75_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPF80146.1"
                     /translation="MPHPVFDPLIHEPTRLQLCGLLARVEESDFATLREELAVADSVL
                     SKHLSALKAAGYVTVERRFGAGHGRGYAVLTDAGRHAFCGHIAELQRLARVASPRGRR
                     DRVRSVP"
     gene            14655..15536
                     /locus_tag="PKU75_04390"
     CDS             14655..15536
                     /locus_tag="PKU75_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPF80147.1"
                     /translation="MTIIAAADGSALGNPGPAGWGWFIDKDTWASGGWRHGTNNIGEL
                     TAVLDLLQQTAGTDEDLMIWCDSTYVINSITKWMPGWKAKGWVKKDGKPVQNLEIMKA
                     LDAAMAGRRVQFKWVKGHTGHELNEEADRLATAAALACQQGRTPDAGPGFTVHPGRKW
                     AHAQETLDAPDTPAVTGDAPTSPEAEVIELERALLTPAVRSNAKAMSSLLHPQWSEIG
                     SSGRHWTREEALTLGPLPGPVSLDVISAERLSDELILLIWRSTSESGSRLRSSLWQRT
                     PAGWQQRFHQGTPEPQE"
     assembly_gap    15737..15836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15912..16685
                     /locus_tag="PKU75_04395"
     CDS             15912..16685
                     /locus_tag="PKU75_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPF80148.1"
                     /translation="MTETTQDLLTVGQVAETFAVTVRTLHHYDEIGLLTPRERTYAGY
                     RLYTASDLERLATIVVYRRLGFGLEEIAELLAGEGDLVEHLRRQRAAVTTRLTELREL
                     VTALDHALEAEMGNRPATTEELKDLFGDGFDESYAAEAQERWGDTPAWTQSQARTATY
                     TKADWESVKAEMDAVNAAFVAALESGEPPTSAAAMDAAEAHRRHINDRFYDVSYAMHQ
                     GLGEMYVTDARFTQTYEDLAPGLAAYVKAAIDANAARQQ"
     gene            complement(16695..17186)
                     /gene="ispF"
                     /locus_tag="PKU75_04400"
     CDS             complement(16695..17186)
                     /gene="ispF"
                     /locus_tag="PKU75_04400"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPF80149.1"
                     /translation="MTGIPRVGIGVDVHAFAPEGSDRPLWVAGLHWPGELGLDGHSDA
                     DVAAHAACDALFSAAGIGDLGAHFGTSRPEFAGASGVTLLAEAARLVREGGYEIGNIA
                     VQVIGNRPKIGTRRDEAQTALSAAAGAPVSVSGTTTDGLGLTGRGEGIAALATALVWP
                     TAD"
     gene            complement(17183..18433)
                     /gene="ispD"
                     /locus_tag="PKU75_04405"
     CDS             complement(17183..18433)
                     /gene="ispD"
                     /locus_tag="PKU75_04405"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPF80150.1"
                     /translation="MSDAGTAPRGVGVVVVAAGSGTRLGAGIPKAFVDVGGRRIVDWA
                     VDGARAVPGVVHVVVVVPPAYVEMPIPPRRDVDVEPDPELTEALYAGAAADRVPVTVV
                     AGGAERSDSVRAGLAALGTDIDIVLVHDAARCFTPAAVYERVIAAVVAGDRAVVPGLA
                     VVDTMKVVGPDGLVVYTPDRSRVRAIQTPQGFARVALEQAHASGVHASDDAMLAEECG
                     IPVRVVDGDPLAFKVTTPADLDQAHKLLASAPTLWVIGGLPGTGKTTAGRVLAAEEGA
                     THLRVDSIEVALLGAGIDEVTSEGYAALYAVARDQLRLGGSVVADLVNPIPLTREALR
                     AVARDAGASIREIQLVCSDAAEHRRRVENRAADIPGHRLPTWADVEARQFEAWPESVT
                     IDTAGLDVEAIVRQIRGRVPEGRG"
     gene            complement(18454..18936)
                     /locus_tag="PKU75_04410"
     CDS             complement(18454..18936)
                     /locus_tag="PKU75_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPF80151.1"
                     /translation="MVFKVGETVVYPHHGAALIEEIKTRVIKGEEKLYLKLKVAQGDL
                     TIEVPAENCDLVGVRDVVGQEGLDRVFEVLRAPYTEEPTNWSRRYKANLEKLASGDVI
                     KVAEVVRDLWRRDKERGLSAGEKRMLAKARQILVSELALAEKTDEVEAESILDGVLAS
                     "
     gene            19117..19596
                     /locus_tag="PKU75_04415"
     CDS             19117..19596
                     /locus_tag="PKU75_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80152.1"
                     /translation="MNSPSTSRRGRAMLATVAVVAGTGLLGGCQLISPRQTDEMYDAA
                     DGVSVDAGDVQVRNLVVVRDRGAASGVLSGAIGNPGAEPVSVTFATADGGSAKVDVPA
                     YTTLDMSTGDSAKVSLPVKDEPGSMTALTVSTPAAGNSPVSVPVLPATGWYADYKAS"
     gene            complement(19695..20381)
                     /locus_tag="PKU75_04420"
     CDS             complement(19695..20381)
                     /locus_tag="PKU75_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF80153.1"
                     /translation="MTPARILVVEDEPSYSEPLTYLLRKEGYQVQVAETGTEGLAAFD
                     RTGADLVLLDVMLPGMSGVDVCRNLRAKSNVPVIMLTAKDSEIDKVVGLELGADDYVT
                     KPYSSRELLARIKAVLRRGAEPEELLPSTVSAGPVSIDVERHVVSIRGDRISLPLKEF
                     ELLEFLVRNNGRVLTRGQLIDRIWGSDYVGDTKTLDVHIKRLRKKVEFTPKEPVHILT
                     VRGLGYKFEA"
     gene            complement(20378..21535)
                     /locus_tag="PKU75_04425"
     CDS             complement(20378..21535)
                     /locus_tag="PKU75_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF80154.1"
                     /translation="MDPTNAALLGGLAGVVIGSMSGFAVRASEKSEVRMTQVPVAPRI
                     PPGASAVLAVLRSSALVLDTAERVVNTSPSAVAMGLVDEDRLVQPQLLELVRQVHRDG
                     EIRETELSIGAGRGRPPAWLHVRAAPLMDAHVLLLVDDRTSAHRVEEVRRDFVVNVSH
                     ELKTPVGGLSLLAEAIADAKDDPEAVERFASRMQLESARLGKLIKEIVDLSRLQASDA
                     LQEPKRVRLAEVIREAVDLSLVEADAKQIRIVQRADPAAIAYGDHGLLVTAVRNLIGN
                     AIAYSPEQTEVGVAGQVSAEGVTITVADRGLGIPVAEQERIFERFYRVDSARSRSTGG
                     TGLGLAIVKHICANHGGSVTVWSREGEGSTFTIRLPHPPASASTSAEGSAA"
     gene            21700..>21937
                     /locus_tag="PKU75_04430"
     CDS             21700..>21937
                     /locus_tag="PKU75_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPF80155.1"
                     /translation="MRTAYHEDLDRISEQLVEMTRLAGSAMSRASRALLDADVQIAET
                     VISADQEIDQAREDLDTLAIDLLARQAPVATELRQ"
BASE COUNT         3230 a         7544 c         7587 g         3476 t
ORIGIN      
        1 ctgccgccgc ccagagaact gggcggcagc atcagaggag gcagtcgaca tcgatcagcc
       61 tcagttgccc cagcagatct tggcgccctc gtccgcggtg atgctctcga caccgtccgc
      121 ggcggtgtcg gtcaccagcg ccacgccggt gttgtagaag tccaggccct cggtgacggc
      181 cggcttctcg ccgccgcggg cgatcttggc gatcgcctcc atgccgaggg tggccatctt
      241 gagcgggtac tgctgggcgg tggcgccgat gacgccgtcc ttgaccgact gcacgccggc
      301 gcagccaccg tcgatggaga cgatcatggc cttcttgccc tcggcctgca gcgcggcgtt
      361 ggcgccgacg gccgtcggtt cgttgatcgt gtagacgacg ttgacattgg ggttcttggc
      421 gaggcacttc tccatcgcgg tgcggccgcc gtcctcggcg ccgttgccgg gctcgttgca
      481 gacgatctcg tagtcgccgc cggagtactt gccggtcggt gcctcgtcgc cgttcttctt
      541 gccgtcggcg acgtcgatgc ccatgccctc gaggaagccc tggtcgcggt tgtagtcgac
      601 ggagacgatc ttgtcgttga agaggtcgag caaggcgatg gtggccttct cgcccttcag
      661 ctgcgcggcc gcccacttgc cgataagctg gccggcgagg cgattgtcgg tggcgaaggt
      721 gatgtcgacg gtgtcggccg ggtccggcgg ggtgtcgagc gcgatgacgt agagccccgc
      781 gtcgcgggcg ttcttgatcg cggcgttcac gccctccgac atgggggtga tcaggatgcc
      841 cttgtcgccg cgggcgatgg cctgttcgat ggcctcgatc tggccctggt cgtcgccctc
      901 ctgcttgccg gaggcaatgg tcagctcgac gttgttcttg gcggcgtcgg cctgcgcacc
      961 cttctgcatg gcgacgaaga aggggttggt ggagtccttg gtgatgagcg cgacgccgac
     1021 cttctcgccg gagctgccgc caccggtggc cgagccggag cccgaggcgg ctcccgagct
     1081 ggtggggtcg gatccgccgc cgcaggcggc gagcgctagt cctagaccga gggctccgac
     1141 ggtggcggtg atgcggcgcg cggcctgctg ggaacggacc atcttcattg atgcgacctc
     1201 tcagttgggc gcggtgaccg cgccttccgg tgacgcgaac ccctgtgccc gcgcctgaca
     1261 acgttgtcga gaggttttct accgcgtgct cttgcacggc gtcaaccgat cgagtacaaa
     1321 atgtgatgtg gacgtgacaa cgttgtcagg tggccagggg tgaatgaagc acctcggccc
     1381 cggcgcgcag ccccgggacg ctcgaccatg cgggatgtcg cggcggccgc gggggtcagc
     1441 ctcaagacgg tctcccgggt cgtcaacaac gagccgggcg tcaccggggc ggtgcgcgag
     1501 cgggtccagg ccgcggcgga gcgtctcgac tatcgtcaca acctcgcggc gagcaatctg
     1561 cgcaggtccg gttctcgcac cggggtgatc ggggtgctgg tccaagacgt cgccaacagc
     1621 tattccgccg gcctgctgcg agccctcgag gatgccatgc gggtccacga cgtgatgctc
     1681 gtcgccggca gcctcgacga gaccaccgcc cgcgagcgag ccatggtcac gaacctgatc
     1741 agccgccgcg tcgacgggct cgtgatcgtg ccggccagtc cccgtcagga ctacctcgaa
     1801 ccagagatcc gcgccggttt gcccatcatc ttcgccgacc gggcgccacg cgggatcgac
     1861 accgactcgg tgatcgtcga caacgccctg ggcgcctacc aggcggtcga ccatctgctg
     1921 gcacgcggcc accggcggat cgccgtgctg accgattcgg cgagcatcgc gaccgccgac
     1981 gaacgcgtcc gcggagcccg cgccgcgttc gcgaaacacg gcctggaact cccgggcgac
     2041 ctgctcgtgc gtggccacgc ggaccagccg ggcgccgccg cgaccgtgca ccggctgcgc
     2101 gaactgcccg ccccgccgac ggcgatcttc gccgggcgca acgcgatcag caagggcacg
     2161 ctgcaggcgt tgtccgagcg gggggagcgc gagagcgtcg ccgtcgtcgg cttcgacgac
     2221 ttcccgatgg ccgacctgct ccaggtcacg gtgatccgcc aggatgtcgc caagctcggt
     2281 gcgctcgtgg cggcccggct gctggagcgc atcgccggcg acacgacacc gccgatccgc
     2341 atcgtcctgc cccccaccct gatcccccgc ggctcggggg agatcccccc gcccgctgcg
     2401 taagaacgcc gaagggccac cccctgcacg ggagtggccc tcggcgtcga agcggcctca
     2461 gtacttcggg gcgctcatgg agttgtagta ggcggaggat tccttcttcc acatcatcgc
     2521 catcgcggcg gcaccgatga ggatgctcag gacgctgagc acgcgcgaga tcagcggctg
     2581 cgtctgggtg aaggagaaga ggaagctcag cagtgagatg acgaaaaaga ccgtcgcgac
     2641 gatgcgcgcc cacgacttgc ccttgccgtt ggccgacgcc atccagaacc acagaccggc
     2701 accgagcagg ccgaagagga tgccgacgcc gaggccgacg ttgacggcag catcgatcgt
     2761 ggactgcgac accccggcgt cgttctcttt catctgcttg gtcacctcgt cgcggagggt
     2821 gtccatcatc aggaacgtgg ccagcaggct gaggatcgag agcacaccgc cggcctgcat
     2881 gagcttgacc gccgtgtcca tcgcactcgg gcgggccatg ggcgccggcg gggtgtagcc
     2941 ggagccgggc atcgcgttca taccgccgta tgccgggggc accggcgcct gcccctgcgg
     3001 gtaggcgccc tggttgccat acgccggcgg cgggggcggc ataccaccct gctcctgcgg
     3061 gttgttcggg tcgtagttcg acatcgcgtt tccttgcgtt ctgccggccc ccaaggtggc
     3121 gaccgttcgg acgtgacgca tcgatcgtat gccgaccggc gtggcgaatc cgggaaccgt
     3181 ggcggctcgc gccgtcaggc cggtcgtcgg gagcgggcga tctcgtacag ggtcacgccc
     3241 acggccatcc ccgcattgag cgactcgacg gcgctcgtca tcgggatgga gacgatctga
     3301 tcgcaggtct cgcggaccag ccgcgagagg cccttgccct cggaaccgac gacgatgacc
     3361 agcggctcgc tggcgagcgc cagctcgggc aggtcgacgt cgccgtccat gtcgaggccg
     3421 acgacgaaga agccctgctt gcgatagtcc tgtagcgccc gggtcaggtt ggtcgcctgg
     3481 gcgacgggga tgcgggcggc cgcgccggcg ctggtcttcc aggccgaggc ggtcatcccg
     3541 accgaacggc gcgacggcac gaggactccg tgccctccga atgccgcgac ggagcggatg
     3601 atcgcgccga ggttgcgcgg gtcggtgatg ccgtcgaggg cgacgatgac cggcacgccc
     3661 ggcttttcgg ggtcgatcag gtcgccgggg tgggcgtagc tgtatgccgg gacctgcagt
     3721 gccagcccct ggtggacggc atcgtcggtg atccggtcga gttcggtgcg cggggtctcg
     3781 agcatgggta tgccgcgctc ccccgcgatc ttgatgacct cgcgaacgcg gtcgtcggag
     3841 tcgatgcggc tggcgacgta gagggtcgag gccgggatgt cggtgcgcag cgcctcgagc
     3901 accgagttgc ggcccgccac gatctcggtg ccgcccttgg cttttcgctg cggggagcgg
     3961 gatccgccgg cgggtcgggt gccggtgccc gggcgcgctg ccccggcctt ctgcgcggcc
     4021 ttgtgggcct tgtggttggg ccggtcggcg gccttgggcg tggggccttt gccttcgagg
     4081 cccttgcggc gctgcccgcc ggagccgacg agggcgccct tcttggaccc tggcttgcgg
     4141 gtggcgccgc gtcgggaact gttgccgggc atcagttctc gcccccgtgc ttgaccaggc
     4201 tccaccgggc cccggtcggg gtgtcgtcga tctggatgcc gacggcgtgc agctggtcgc
     4261 ggatcgcatc ggcggtggcg tagtcgcggg cggcccgggc gccggcgcgg gcggcgagct
     4321 gggcctgcac gagggcgtcg agagcatcgg cggcctcgcc ctggccggcg gtgtccgagc
     4381 cggaccagtt cggcgccagc ggattgacac cgaggatgtc ggtcatggcg atgacgagcc
     4441 gtgcctcctg ggcggcgacc tccgcctcgt cctcgtccag cgcgatattg ccggtgcgca
     4501 cgtgctcgtg gacgaccgcg agggccccgg agacgccgag gtcgtcatcc atggcggcga
     4561 cgaaatcctc cggcaggatc tccgtgcccg tcggctgtgc ggtgtgctcg ccgggcagcg
     4621 cgcggcgcag gaatccctcg atccggtcga ccgccgcctc ggcctcggcg agggagcccg
     4681 cgtggaactc gatggtcgag cggtagtggg cggcggtgag gtagtagcgc acggcgagcg
     4741 ggcgggcgtg cttggtcacc tcggcgatca ggagcgagtt gccgagcgac ttgctcatct
     4801 tctcgccgct gatcgtcacc cacgcgttgt gtagccagta gttggcgaaa cccattccgg
     4861 cggcacggga ttgggcctgc tcgttctcgt ggtgcgggaa gcgcaggtcg acgccgccgc
     4921 cgtggatgtc gaaggtgtcg ccgagatatt tgcgcgccat cgccgagcac tccaggtgcc
     4981 agccggggcg gcccaccccg tagggcgtcg gccagctcgc gctctccggc tcggtcggct
     5041 tgctgccctt ccagagggtg aagtcgcggg gatcgtgctt gccgcggggg tcggcgtcct
     5101 ccgccgccgc catgtcctcg accttctgcc gcgtcagttc gccgtaggcc ggccagctgc
     5161 gcacgtcgaa gtagacgtcc ccgctgccgt ccggggccgg ataggcatgc cccttctcga
     5221 tcagcacccg catcagctcg atcatctcgg gaacgtggcc cgtggcgcgg ggctcgtagg
     5281 acgcgggcag caccccgagg agctccagcg cctcgctcgt gagtcgctcg ttgcggtagg
     5341 acagcgcgaa ccagtcctcg ccggcctcgg cggacttggc gaggatcttg tcgtcgatgt
     5401 cggtgacatt gcggatcaac gtcacgtcgt agccgtgccc gcgcatcagc cagcggcgca
     5461 gcacgtcgaa ggccacggcg aagcgaacgt gcccgatgtg cggcggcgcc tgcgtcgtca
     5521 ggccacagat gtagatgccg acctggccgg gggtcaccgg gacgaaatcc cggatctcgc
     5581 gggcggcgga gtcgaacagg cgcaaagtca ccggtcaagg ctacctgcgc cccgtccctc
     5641 cccgcctccg cctattcatg cgtagcccgg gcggaagatc acattctgga cactcgtcca
     5701 gtttcttgcc cgttggagat gcgtaatccg ccccgtggtg ggacgctgag ccgatcgggc
     5761 agtactcccg cgcggaatgg ggattcccat gagtgatgtc agccgtccgt ccagtctgga
     5821 cgcggagcag gtgggctaca agcagaccct cggacgccgg cacgtccaga tgatcgccat
     5881 cggcggcgca atcgggaccg gcctcttcct ggggtctgcc tcccggctca acagcaccgg
     5941 tccggccctg ctcttcagtt acgccttcgt cggcgtcatc gcctacttcc tcatgcgcgc
     6001 cctcggtgaa ctcgtgctgc accgcgccac ctctggcgcc ttcgtctcct atatgcgcga
     6061 gttctatggc gagttctggg cctacctgac cggctggatg tactggatca actgggccct
     6121 gaccggcatc gccgaactgt ccgccgtcgg cctctacatc cagcactggt ggccggactt
     6181 cgcgacgtgg aagaccgtgc tgatctccct ggccgtggtg ctcatcatca acctgctctc
     6241 ggcccgggcg ttcggggagt tcgagttctg ggcatcggtg gccaaggtcg ccgcgatcct
     6301 ggtcttcctc gccgtcggtc tcgtcgtcgt gatcggccgg atggacatcg gtgaccacag
     6361 ggcgggtttc tccaacttgt ggtccaaccc cggcggcttc tggccgcata ccgacggctt
     6421 caactggtac ggcccgatca tggtgatgtc cggtgtcgtc ttcgcgtatg ccgcgatcga
     6481 gatggtcgcc gtcgccgcgg gcgagatgga ggatgccgag cgcgaggtgc ccaaggccgt
     6541 caacgccgtc atcatgcgga tcgccgtctt ctactgcggc tcgatcctgc tcctggtctc
     6601 gatgctgccg acgagtgaat acaaggcggg catcagcccg ttcgtcaccg tcttcggccg
     6661 gatgggcctg gactggatgg gcagcgtcat ccaagcggtg ctgatcgtcg ccgcaatgtc
     6721 gtcgctgaac tccgggctct actcgaccgg ccgggtgctg cgcagcctcg gcgtcgccaa
     6781 gcaggcaccg gccttcacgt tgaagatgag caagtccggt gtcccgtggg ccggcatcgt
     6841 catgacctcg atcgtctacc tgttcggtgc catcctgaac gccctcaccc cgcacgcctt
     6901 cgagatcgcg ctggaggcgg ccgcgctcgg cgtgctgttc acgtggggga cgatcttcgc
     6961 ctgccaactg cggctgcgca aactcgtcaa caagggggtc atcccaccga gtcccttcca
     7021 ggcgcccggc tatccatgga cgagctatgt cggcctggcc ttcctcgtgt acgtcgtcct
     7081 cgcgatggcg atcagcggat ggcagtccag tccctacttc tggggcaaga ccaacttcat
     7141 cgtcgttgtc atcggcatcc cgctcatcgg tgcgctgctc ctcctcgggt ggatgatcgt
     7201 gcgccccaag gtggttgcca ataccggcaa ccggctcggc cccgtttggt ccgacgacgg
     7261 cccgacgtat gcggatgccc gccccgtcga acccgcccaa cacccctgag cgagaagggt
     7321 aaccgacatg aactcctgga accgacgcac cgtcaccctc ctcgcggggc tagcggtcgt
     7381 tgccggcgcg ggcgcctgcg actccggctc gtccccgacc acatccacga cgcccacggc
     7441 cagcggcacc gggacgatca agatcggcgt ctcgaccgac gagccgggtg tctcgctgaa
     7501 ggagggcaac acctacagcg ggttcgacat caagaccgcc gagtatgtcg ccggcaagct
     7561 cggcctgacg ccccaatggg tgccgatcgc ccaggcagac cgcgtcagtg cgctgcagag
     7621 cggtgaggtc gagatggtcg tcgcgacgtt ctcgatcacc gacgagcgca aaaagcaggt
     7681 cgacttcgcc ggtccctact tcgtcgccca ccaggacctg ctgatccggc tcaacgatca
     7741 aacgatcacc cggcccgagc gcctcgacgg caagatcttg tgctcggtca cggggacgac
     7801 ctcggccgca tacgtccagt cacactatga caaggccaag atcacgctgc gcgaagtgcc
     7861 gtccttctcc gagtgcgtac gcaacctggc caatggcgac gtggacgcgg tgaccaccga
     7921 cgacctcatc ctcgcgggct tcgccgccac ccccgaatac aagggcatct tgaaggtgct
     7981 cggcttcggc ttcaccgacg agacctacgg catcggcatc aagaagggca acaccgacct
     8041 ggtcaccaag gtcaacgggg cactgaagga gtacatctcc tccggcgcgt ggaagcaggc
     8101 gctcgaggag acggtcgccc cgtccgggta caagatcccc aatccgccta cggtgggcta
     8161 gtgcacgacc gcgacgacct gagggcagtt ctcgagacgc acgagccgtt cagttcggcc
     8221 tcgcccgagg tccgcgagcg tgtgatcgcc agtgccgaga ttgcacagta tgccgctggc
     8281 acgctcgtcc tcgacgcgtt ctccgacccg tcctcggcgg tgtatctcat cctcgacggg
     8341 caggtgggcc tgtggaatga cgcccgcaaa cgacccctcg aaccggacga aatcctcggc
     8401 ccgggaaggg ttttcggctt ctcggcgatg atcaccgaac gatcggtggg accgaaggcc
     8461 atcgcgttga cgcaggcgcg ggtggcacgg ttgccgggcg agcacgccgc cgccgctttc
     8521 gtcacccggc aaggtgctcg cttcctcgcg gacaacctgc tgccgcatca cgaggacacc
     8581 ttgccgacgc gggcgacggg gtaccagcgc ctcggcgacc tgatcgtcga gtcgcccctg
     8641 caggtccccg cctcggcgtc catcgcggag gtggccaagg cgatgaccga tcagggccgc
     8701 acctgcgccg tcgtgaccct cgatgacggc ttcggcctgg tcaccgacgc ctcgctgcgt
     8761 gaacgggtcc tcgtcgacgg cctgccgccg actgctccgg cgcgcgacgc tctggaccgg
     8821 accgcgctgc atacccccga ggacacctcg gccgccgagt cactcgtcgg cctgatcgag
     8881 gccgacgccg aggcgatcgt tgtcactacc caggacggcc ggctcgcagg ggtcgtcacg
     8941 atgcgcgaat tcgcgttgtc tcctactgcc gccgatctgg ctgtgcacca acagcttcgg
     9001 cgggcaccga cgagcgcgga cctgacccgc cacgcccagg agatcccgtc gctgctgcgc
     9061 catctgctgc gccgcgggct cggctccggg cgcgtgatcg cggtctattc gagcctgatc
     9121 gacgcgatca tccaccgggc catcgagatc gaactcgcca agcgccccga cctgcctgcc
     9181 gacggcttca cgtggtacgc cctgggcagc aacggccgcc gcgaagcggt gttgtcctcg
     9241 gacgtcgatt ccggggtggc cttcgccgac ggcacgagcc cggagttgat gtcggcctat
     9301 cgcgaggtct ttgcccaggt cgacgtcgtg ctcatggacg ctggcttcac cggagacgcc
     9361 catggcgtga gcgcccgaca cgagatcctg tcgcgcacgg actcggagtg gctggccgcg
     9421 gcgcgtggct ggctcgccga cccggtcgcc gacaaggggg cgatcatgac ctccttgctc
     9481 gtcgacagcc ggcccatcac gggggcccag cggcgggtgg cggccgagtt cgttgcgagc
     9541 gagctgcggt cgcatccggg cacgatgcgg ctgctgctgc tggatgccct cgaccggcgg
     9601 gcgaagtggc gctcgatgct ggagggctgg cgcgggccgg tgcgctacga cctgaaggat
     9661 cacgcgattc tgccgctggt gaacttggcg aggtggggcg cgttggcggt ccgctcgacg
     9721 gccctgtcga cgccggatcg gctgcgagcc tgcggcggga cctcgattct gcctcaggct
     9781 caagcgaatt cgctgatcga ggtgttcgag gtgctgcaac ggctgcggct gcgctatcag
     9841 ttgttgcagg tcgccgacgg cgcgcgggcg agcgacgcgg tgctcatcga ccagctctcc
     9901 ccgatcgatc gcagcgtcgt cgcgcaggcc gtgcgggaga tcgcgaccgc gcagaagcgg
     9961 ctggcgaact tctccgccta cgtcgacacc gacgagtgga cccgcccagc ccaggggtga
    10021 gcccgatgac gatcgagcgc cggtatgccg tcatcggcga tgtcgcgggg cattatgcgg
    10081 acctcacgca ggagttgcgc cggctcggcg ccgacccgga ggcggggacc ctgccccccg
    10141 atctgatcgt cgtccaggtc ggcgatctga tccaccgcgg gccgcagtcg gacgccgtgg
    10201 tggcgctggt cgaccacttc ataagcaccg cgccgcaaca gtggatccaa ctcatcggca
    10261 atcacgaggc gcactacgcg cgcgcccagg tcttcgagtg gcacgagcag atctcggcac
    10321 gcgctatctc cgcaatgcgc gcctggtggc acgagggcat catgcaggtt gcgacggtcg
    10381 tgccgagcgc gacgggagac ctgctcgtca cccacgccgg ggtgaccgag ccgttctggc
    10441 gggaggacct cggtgctctt cccgacccgt atgccgtggc cgagcgcctc aaccagatgg
    10501 ggcgcttggg ccggaaggcg gtcttccggc ccggcgagat gctcaccgac cgcgttgcgc
    10561 cacgggtcgg tccgctgtgg gcctccgcgg gcagcgaact ggtggccagc tgggtcggcc
    10621 accgcctccc cttcggccag gtgcacggcc actcatcctg ttacgactgg cagcgcagcg
    10681 cctggcgccc gtccgtgccc tccggtgccc acatccacct cgaccacgac gccggccacg
    10741 aaaccgtcac cctcgacggc ggcgtcatca tcggcgtcga ccccggccac ggcgcccgcg
    10801 cccacaccgc ctggcgcgcc tgggtgagcc aaggaacctc tgctagcaga ggctagtcac
    10861 atcagcagac cttgctaggt gtgcggattt gagcaacctc tgctgagttg gcgctctgct
    10921 gagtcggcgc cgatcgccgt gcgcgacagc ggcgttggcc cgcgccacat tcaacgacag
    10981 ccctttcttg gcccagccac tccggcggac cactcacagg ccgcgccccg cggcgctccc
    11041 cggcgcaggc cgacggcggc ggccctcggc gctacttctc gacgaccacc gtcacccggc
    11101 ggttgagagc ctggcccgcc ttggtctcgt tgctggcgac cgggttctgc tcgccatggc
    11161 cggccgaggt cacggtgacc ttgccgccga gcgccgcctc gaggcggtcg cggacggcgt
    11221 cggcccggcg ctgcgagagc cgcaggttgt atgccgtctc tcccacgtcg tcggtgtagc
    11281 cgtcgatccg cacgcgcgtc gccttggccg cctcgagctt gccgaccaat tcggcgagcg
    11341 ccgcctcgcc tttgggcgtg atgtcggact cgtcgaaccg gaacaggacg ttggcgtcga
    11401 gccggaaccg cttgccgtcg aagtcggtca gcgagccgtc gagattggcc gtcggcagca
    11461 ccagcgtctg gacctcggcg gtgatgtcga ggacgggcag ttgcgcgggc gtgccctccg
    11521 cggggacgac caccggcggt gcggtcgggg cgccggatgc gggcgccgtc agcccgatcg
    11581 ccagcgccgc aaccaggatt cgcgccctca tgcgcgcgtc accggcacct tggcaaacgt
    11641 gcccacgttg gggatggaca cgtcgaccgt ggtggtgctg tccggcgggg ccgcgaagac
    11701 cgcctccagc acgacgtcgc tgccggcccc gattacctgt gccccaaggg agctgcacac
    11761 gcagtggccg tccttgtctc gcgccggcag gtagcgcttc ttggcggcgg catcgagcac
    11821 atagacgccg tcgacgattc ctgctcccgc ttccagcgtc ttgtccagct tgccctcgtc
    11881 gggattgccg gcgtagaagt agttccccaa catccagctc tccgcggagt cgctgcgtgc
    11941 ggagaatgtg acggtcatca ggtcggcgtt gaccgtcacc gagttcaacg acacctccat
    12001 cccgttgccg ttgccatcct tcgcgccctt gcgggtgccg atgacgggca ggggttcggc
    12061 agccggcccg gtggccgagg cggattggct cgccggctgg gggacgctca catcgcccgt
    12121 ggccgcgctg ccggtcgacg ccgtgtcggc cgccgcggag cccgccgcag agccctggtc
    12181 ggtcgcggtg acggtcaccg gtgcggtggc cgtgtccgat gatccggtgc acgccgacat
    12241 accggcgatg gcacccaacg ctgccacgag ggtcacggtt cgagatgtgc gggaacgggt
    12301 tgcgagagaa gaggaacggt tcacgaggcg acctccggtg cgggcgcggc gagcaccccc
    12361 tgcggtgccg ccgtcgcctc gaacctaggg cggattcgcc gatcccgcac ccggaacgca
    12421 gccggtgtgt ggataactcg ggtcaggcga agtcggagcc ggtggcctcg gcgccgatcg
    12481 tgctcgaatc gccgtgaccg gtcagcacga ccgtttccgg cggcagcacc aggaggctgt
    12541 cgcggatgga ggcgaggatc gtcggcttgt cgctgtagga gcggcccgtc gcgcccggcc
    12601 caccctggaa gagggtgtcg ccggtgaaga ccaggccgag cgaggcggca tacaggcaga
    12661 ccgcgccggg cgcgtgaccg ggcgtatgca gcacctccag ctccacgtcc ccgatcgaga
    12721 ggctctcgcc gtcggcgagc tgttcgtcgg gctcggttcc cggataggtc agctcccaga
    12781 gcacctgatc ggccgggtgc agcgcgatcg gggcgccggt ggcctcgcgc acggccgggg
    12841 cggccgtgat gtggtcattg tgggcgtgcg tgcacaagac cgcccgcacc gaccgatcgc
    12901 ctactgccgc aaggatcgct gcggcatcgt gggcggcgtc gatgacgaaa cactcggcat
    12961 cgtcaccgac gatccagacg ttgttgtcga cctcccactc cccgccgtcc agggcgaaga
    13021 tgccggaggt gatcacccgc tcgacccgca ctccgccggg cgaggtggcc acgacctcgg
    13081 ccctgcccgc ctccgaactc atagctccac caccgagcgc agcacctcgc ccttgtgcat
    13141 cttgttgaac gcagactcga tgtcccccaa cccgattcgc tcgctgacga aggcgtcgag
    13201 cgggaagcgg ccctgctgat agaggctgac gagggtcggg aagtcacgcg tcgggaggca
    13261 gtcgccatac cagctgctct tgagcgaccc gccgcgcccg aagatctcga tgaggggcag
    13321 ctcgagcttc aggtcagggg tcggaacccc gacgagcacc accgtgccgg ccaggtcgcg
    13381 ggcgaagaac gcctgctggt aggtctccgg ccggccgacg gcctcgacga cgacgtccgc
    13441 gccatacgac ccggtcagct cgcggatctt ctcgaccgcg tcctcactcg tggagttgat
    13501 cgtgtgcgtg gcgccgaatt ccttggccca ctgcagtttt cggtcgtcca cgtcgacggc
    13561 gatgatggtc gtcgcgccca cgaccttggc tcccgcgatg gcggcattgc ccaccccgcc
    13621 gcagccgatg acggcgatcg aatcgcccag cccgacgcct ccggtgttga ccgccgcgcc
    13681 gaagccggcc atcacgccgc acccgagcag gccgacgacc gccgggtcgg aggtggcgac
    13741 cttggtgcac tgaccggcgg cgacgagggt cttctccgcg aaggcgccga tgccgagggc
    13801 cggcgagagc acggtgccgt cggtgagcgt catcttctgg gcggcgttgt gggtgtcgaa
    13861 gcagtactgc ggcttgccct tgtggcacgc gcggcactca ccgcatacgg cccgccagtt
    13921 gaggatgacg aaatcccccg ctgccacctc ggtcactccc tcaccgaccg cctcgacgat
    13981 gccggcggcc tcgtgaccga ggaggaaggg gaaatcgtcg ttgatgccgc cctcgcgata
    14041 gtgcaggtcg gtgtggcaga cgccgcaggc ctgcacctgc accaccgcct cgccgggacc
    14101 cgggtcgggg atgacgatgt cggtgacctc gacgggtgcg cccttggcgc gggcgatgac
    14161 gcctttgacg gtctgcggca tgtgcgtact ccttcagtcg gctcccgcct actctcgccg
    14221 cgaccgggcc cgcgtgcaag ggacggcggg cgacggcata ccaaaatcgt tgcgggagtg
    14281 gggaaagccg actttccgca acggaaagac tcgtcgtatg ctgacgttgt gccccatccc
    14341 gtgttcgacc cgctcatcca cgagccgacg cgcctgcagt tgtgcgggct gctcgcgcgg
    14401 gtcgaggaga gcgacttcgc gacgctgcgg gaggagttgg ccgtcgccga ctcggtgctc
    14461 agcaagcacc tgtcggccct caaggccgcc ggatatgtca ccgtcgagcg gcggttcggg
    14521 gcaggtcacg gtcgcgggta tgcggtgctg acagacgccg ggcggcacgc cttctgcggg
    14581 catatcgccg agttgcagcg gctcgcgcgg gtcgcctcac cgcgcgggcg ccgggaccgg
    14641 gttagatcag tcccgtgacg atcattgcgg cggccgacgg ctcggccctg ggcaaccccg
    14701 gtccggcggg ctggggctgg ttcatcgaca aggacacctg ggccagcggg gggtggcggc
    14761 acgggaccaa caacatcggc gagctgaccg ctgtgctcga cctgttgcag cagacggccg
    14821 gcaccgacga ggacctgatg atctggtgcg actcgaccta tgtcatcaac tcgatcacca
    14881 agtggatgcc cggctggaag gccaaggggt gggtcaagaa ggacggaaaa cccgtccaga
    14941 acctcgagat catgaaggcg ctcgatgcgg ccatggccgg gcggcgggtg cagttcaagt
    15001 gggtcaaggg gcacaccggt cacgagctga acgaagaggc cgaccggctc gcgaccgccg
    15061 ccgcgctggc gtgccagcag gggcgcactc ccgacgccgg gcctggattc accgtgcatc
    15121 cggggcggaa gtgggcgcac gcccaggaga ccctcgacgc cccggacact ccggcggtga
    15181 cgggtgacgc gcccaccagc cccgaggcag aggtgatcga actcgaacgc gccctgctga
    15241 ccccggccgt gcggtccaac gccaaggcca tgagctcgct gctgcacccg cagtggagcg
    15301 agatcggttc gtccggacgg cactggaccc gcgaggaggc gctcaccctg gggccgctgc
    15361 ccgggccggt gtcgctggac gtcatctccg ccgagcggct cagcgacgag ttgatcctgc
    15421 tgatctggcg cagcacgagc gaatccggtt cgcggctgcg cagctcactg tggcagcgca
    15481 cccccgccgg ctggcagcag cgcttccatc agggcacccc cgagccgcag gagtgatcga
    15541 ggcgaactcc gtcggttagc gcgaagtcct tcggttagcg cgaacagcgt cggttagcgc
    15601 gaactccggc agatatttct gacgccgttc gcggtttccc acgccgttcg cggtttctca
    15661 cgcagttcgc gtcttcccca ggtgggcacc gaggtgcgcc acgaggacga gagcggcgag
    15721 ggcgagggcc cacgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgc
    15841 gaggaaggcc atccccatgt ccacgattat gcgcttgacc ctcacgcgac gtcagcccgc
    15901 agagtagaca cgtgaccgag acgacacagg acctgctcac ggtcgggcag gtggccgaga
    15961 ccttcgccgt gacggtgcgc acgctgcacc attacgacga gatcgggttg ctcactcccc
    16021 gtgagcggac ctatgccggc taccggcttt acaccgcgag cgacctggag cggctggcga
    16081 ccatcgtggt ctatcgccgc ctcgggttcg ggctcgaaga gatcgccgaa ctccttgccg
    16141 gagaaggcga tctggtcgag cacctgcgtc gtcaacgcgc agcggtcacc acccgcctga
    16201 cggagttgcg cgaactcgtg acggcactcg accatgcatt ggaggcagag atgggcaacc
    16261 ggcccgcaac cacggaagaa ctcaaagacc tcttcggcga cggcttcgac gagtcgtatg
    16321 ccgctgaggc ccaggagcgc tggggcgaca ccccggcgtg gacccagtcg caggcgcgca
    16381 ccgccacata caccaaggcc gactgggagt cagtgaaggc agagatggac gcggtgaacg
    16441 ccgcgttcgt cgccgccctg gagtccggtg agccgccgac aagcgcggcg gcgatggacg
    16501 ccgcggaggc gcaccgccgg cacatcaacg accggttcta cgacgtgtcc tatgcgatgc
    16561 atcaagggct gggtgagatg tacgtgaccg acgcgcggtt cacgcagacg tatgaggatc
    16621 tggcccccgg gctcgcggcc tacgtcaagg ccgccatcga cgcgaacgcc gctcgtcagc
    16681 agtgacgtct cgcctcagtc ggccgtcggc cacaccaggg ccgtggcgag ggcagcgatg
    16741 ccctcgccac ggccggtgag gccgaggccg tcggtggtgg tgccgctgac ggacacgggc
    16801 gcgccggcgg cggcggagag ggcggtctgg gcctcgtcgc ggcgggtgcc gatcttgggg
    16861 cggttgccga tgacctggac ggcgatattg ccgatctcgt agccgccctc gcggaccagc
    16921 cgcgccgcct cggcgagcag ggtcaccccg gaggcgccgg cgaactcggg acgcgaggtg
    16981 ccgaagtggg cgccgaggtc gccgatcccg gcggcggaga agagcgcgtc gcacgcggca
    17041 tgggccgcga cgtcggcgtc ggagtggccg tcgaggccga gctcccccgg ccagtgcagc
    17101 ccggccaccc acagcggacg gtcgctgccc tcgggcgcga acgcgtgcac gtccaccccg
    17161 atcccgacgc gcggtatgcc ggtcatccgc gcccctccgg cacgcgaccg cggatctggc
    17221 gcacgatcgc ttccacgtcc aggcccgccg tgtcgatcgt aaccgactcc ggccacgcct
    17281 cgaactgccg cgcctcgacg tcggcccagg tgggcaatcg gtggccgggg atgtcggctg
    17341 cgcggttctc gacccggcgg cgatgctcgg cggcgtcgga gcagacgagc tgaatctccc
    17401 ggatactcgc gccggcatcg cgggcgaccg cccgcagggc ctcgcgggtc agcgggatgg
    17461 gattgacgag gtcggcgacc accgacccac cgaggcgcaa ctggtcgcga gccacggcat
    17521 acagcgcggc gtaaccctcg ctcgtcactt cgtcgatccc cgcaccgagc agggcgacct
    17581 cgatcgagtc cacgcgcagg tgcgtcgccc cctcctcggc agcgagcacc cggccggccg
    17641 tcgtcttgcc ggtccccggc aggccgccga tcacccacag cgtcggggcc gaggccagca
    17701 gcttgtgggc ctggtcgaga tcggcggggg tggtcacctt gaaggcgagc gggtcgccgt
    17761 cgacgacgcg caccggtatg ccgcattcct cggcgagcat cgcatcgtcg ctggcgtgca
    17821 caccgctggc gtgtgcctgc tccagcgcca cccgcgcgaa cccctgcggg gtctggatgg
    17881 cccgcacccg cgaccggtcg ggggtgtaga cgaccagccc gtccggaccg accactttca
    17941 tcgtgtcgac caccgccagg ccggggacga cggcgcgatc ccctgcgacg acggccgcga
    18001 tgacccgctc atagaccgcg gcgggggtga agcagcgggc ggcgtcgtgg acgaggacga
    18061 tgtcgatatc ggtgccgagc gcggccaggc ccgcgcggac ggagtccgag cgctcggctc
    18121 ccccggcgac gacggtgacc ggcacccgat cggccgccgc accggcatac agcgcctcgg
    18181 tcagctccgg atcgggctcg acatcgacat ctcgacgggg cggaatcggc atctcgacgt
    18241 aggccggggg gacgacgacg acgacgtgga cgactcccgg gacggcccgg gcaccatcga
    18301 ccgcccagtc gacgatgcgc cggccgccga cgtcgacgaa cgccttgggg atccccgcgc
    18361 ccagccgggt gccggagccg gccgcaacga cgacgacgcc gacaccgcgg ggagcggtgc
    18421 cggcgtcgga cacgttggat ggtgcgggag gaatcaggag gcgagcacgc cgtcgaggat
    18481 cgactcggcc tcgacctcgt cggtcttctc ggccagcgcg agttcggaga ccaggatctg
    18541 gcgggccttg gcgagcatgc gcttctcgcc ggcggacagg ccccgctcct tgtcgcggcg
    18601 ccacaggtcg cgcacgacct cggcgacctt gatgacatcg cccgaggcga gcttctcgag
    18661 gttggccttg tagcggcgcg accagttggt cggctcctcg gtgtaggggg cccgcagcac
    18721 ctcgaacacc cggtcgaggc cctcctggcc gaccacatcg cgaacgccga cgagatcaca
    18781 gttctcggcc ggcacctcga tggtcagatc gccttgcgcg accttgagct tgaggtagag
    18841 cttctcctcg cccttgatga cccgggtctt gatctcttcg atgagtgcgg ccccgtggtg
    18901 ggggtacacg accgtctcgc cgaccttgaa aaccatatgt atttggcccc tttcgcgacc
    18961 tcaagaataa cacggggttc cgtcggcgtg tctggctcgt cacacaggag ggcattggtc
    19021 ggcgaagcgg tgccatcaga cgtgtccggc gcggtcgtga ggcaattgtg ccgcctgccg
    19081 cgccgaggca ccggcggcac cgctacgctg gttcgcgtga actcaccctc cacctcgcgc
    19141 cgcgggcgcg ccatgctcgc caccgtggcc gtcgtcgccg gcacgggtct gctcggcggg
    19201 tgccagttga tctccccccg ccagaccgac gagatgtatg acgccgccga cggggtttcc
    19261 gtcgacgccg gcgatgtcca ggtccgcaac ctggtcgtcg tccgcgaccg cggggccgcc
    19321 agcggggtcc tgagcggtgc catcggcaac ccgggtgccg agccggtcag cgtgacgttc
    19381 gcgaccgccg acggcggcag cgccaaggtg gatgtcccgg cctacaccac cctcgacatg
    19441 tcgaccggcg acagcgccaa ggtgtcgctg ccggtcaagg acgagccggg ttcgatgacc
    19501 gccttgacgg tctccacgcc ggccgcgggc aactcacccg tgtccgtgcc ggtcctgccg
    19561 gccaccggct ggtacgccga ctacaaagcc tcgtagggca ggcctccctg gcgctcgggc
    19621 agctcgtctg tgccgggagc ccgagctcgc atcgttcgac ggcttgcctg ggcttggttc
    19681 gacggcttgc ctggtcaggc ctcgaatttg tagccgaggc cgcgcacggt caggatgtgc
    19741 accggctcct tcggcgtgaa ctccaccttc ttgcggagcc gcttgatgtg gacatcgaga
    19801 gtcttggtgt cgccgacata gtcgctgccc cagatccggt cgatcaactg gcctcgcgtc
    19861 agcacccggc cgttgttgcg caccaagaac tccaacagct cgaactcctt gagcggcagc
    19921 gagatccggt cgccgcggat ggagaccacg tggcgttcga cgtcgatcga caccgggcct
    19981 gcgctgaccg tgctcggcag gagctcctcc ggttcggcgc cccgccgcag caccgccttg
    20041 atgcgcgcga gcagctcgcg tgagctgtag ggcttggtga catagtcgtc ggcgccgagc
    20101 tcgaggccga cgaccttgtc gatctcgctg tccttggccg tcagcatgat gaccgggaca
    20161 ttggacttgg cgcgcaggtt gcggcagacg tcgacgccgc tcatcccggg caacatgacg
    20221 tcgaggagga ccaggtccgc gcccgtacgg tcgaaggcgg ccagcccctc cgtgccggtc
    20281 tcggcgacct ggacctggta gccctccttg cgcaacagat aggtcagcgg ttccgaatag
    20341 gacggctcgt cctcgacgac gaggatccgg gcgggggtca cgcggcgctc ccttcagcgg
    20401 aggtggaggc ggaggccggc gggtgcggca gccggatggt gaaggtcgat ccttcaccct
    20461 cgcgactcca gaccgtgacc gaaccaccat ggttggcgca gatgtgcttg acgatagcga
    20521 gccccaggcc cgtcccgccg gtgctgcggg agcgggcgga gtcgacgcga tagaagcgtt
    20581 cgaagatgcg ttcctgttcc gctaccggta tgccgaggcc ccggtcggcg acggtgatgg
    20641 tcaccccttc ggcgctcacc tggccggcga cccccacctc ggtctgttcc ggcgagtagg
    20701 cgatggcatt gccgatgagg ttgcgcacgg ccgtcacgag gagaccatgg tcgccgtagg
    20761 cgatcgccgc cgggtcggcc cgctggacga tgcggatctg cttcgcgtcg gcctcgacca
    20821 ggctgaggtc cacggcctcc cggatcacct cggccagccg gacgcgtttg ggctcttgca
    20881 gggcgtcgct ggcctgcaga cgggagaggt cgacgatctc tttgatgagc ttgcccagcc
    20941 gcgccgactc cagctgcatc cgggacgcga agcgttcgac ggcctccgga tcgtccttgg
    21001 cgtccgcgat ggcctcggcc agcagcgaca gcccgcccac gggtgtcttc agttcgtgcg
    21061 agacgttcac gacgaagtcc cggcgcacct cttcgacgcg gtgggccgaa gtgcggtcgt
    21121 cgacgagcag cagaacgtgc gcatccatca ggggcgccgc ccgcacgtgc agccacgcgg
    21181 gaggccgccc gcgccccgcc ccgatcgaca gctcggtctc gcggatctcc ccgtcgcgat
    21241 gcacctggcg caccagctcc agcagctggg gctggaccag gcggtcctca tcgaccagcc
    21301 ccatcgccac cgcgctcggg ctggtgttga cgacgcgctc ggccgtgtcg aggaccagcg
    21361 cactcgagcg gagcaccgcg agcacggcgg aggctccggg agggatgcgc ggcgcgaccg
    21421 gcacctgcgt catccgtacc tcggatttct cggaggcccg gacggcgaaa ccggacatgg
    21481 atccgatgac caccccggcg aggccgccga gcagggcagc gttcgtcggg tccacggcac
    21541 ccaagcgtac ggtgctagtc ggacctgttc gggaccgcgg acaaggatcg ccgattcggc
    21601 tcccgccgtt cacctgagag acggaatacg ttcaccggtg cctgatcaga tgctcatcgc
    21661 gggcgctggc cgtccgctcg tcgacgccaa aggacaccga tgcgcaccgc ataccacgag
    21721 gatcttgacc gcatcagcga gcagctggtg gagatgacgc gtctcgccgg ctccgccatg
    21781 tcgcgagcca gcagggcgct gctggacgcc gacgtgcaga tcgccgagac ggtcatctcc
    21841 gccgaccagg agatcgacca ggcccgcgag gacctcgaca ccctcgcgat cgatctgctg
    21901 gcgcggcagg cgccggtggc gaccgagttg cgtcagg
//
LOCUS       DAOPJG010000028        21643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2579_length_21643_cov_7.786131, whole genome shotgun sequence.
ACCESSION   DAOPJG010000028 DAOPJG010000000
VERSION     DAOPJG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_21643_cov_7.786131"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PKU75_04435"
     CDS             <1..822
                     /locus_tag="PKU75_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPF80156.1"
                     /translation="RIIAVINDQMPYTYGDAQVPVDLVDYAIEVSVPLPEITPATPDA
                     DSNRIGELVAARIPAGATLQAGIGAIPDATIAQLHTHKGLRIWTETFSDGVLALERGG
                     SMDPNHPLTTSFLMGTRELYDWVDGNPRVRMMRTERTNDPALIALQRHMTSINTALEI
                     DLFGQANASRINARIYSGFGGQTDFIVGALHSPGGAAYMALRSWHPKANVSTIVPLVD
                     EPVTSFQQSAVVTENGVAELWGYTQQAQARHLIRDAAHPDVREELWEEARELGLA"
     gene            1139..2665
                     /locus_tag="PKU75_04440"
     CDS             1139..2665
                     /locus_tag="PKU75_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF80157.1"
                     /translation="MSTSAPEDFAQALAQRLGGQSVRQLAAVSGWGRTTVSDIRAGRH
                     IPSEAQLRDLLMSVGADESEIASWQERRSAIITPPAAPAAPPTLPVTPPAPPVTPPPV
                     PRRRLARAAVAAAGAISLLGIGFAGGYAARDRAGAHAQQVSAAANPGRISGTRSAVVE
                     VSQGASHLCVRTAGGAIECMGRNKVGQLGDGLRVDSAVPVPVGGLLAGAKRISAGGDF
                     ACVINFDDQAKCWGHNAYGQAGNGQETMLLTPTLVEDLPDVKEIAVGNLHACALTLAG
                     DAYCWGNGEHGQLGIGRFLRNAARPVLVQGIEAELAGITAGRDFTCAWSTRSEVYCWG
                     AGSYGQLGIGTRQDEPTPVRLEKAYPLGVRAMAAGYDHVCAIAPGGGVDCWGRGDSGQ
                     LGTGSTEDALTPRPVVGLDAAEALAASIRHTCAVTPTGAVACWGDGASGQLGPQSDGS
                     ARPVIVPGVSGIRKVSAGQHSSCGISADALWCWGASRYGEIGAGSRENLAQPHRVAIS
                     "
     gene            2800..5055
                     /locus_tag="PKU75_04445"
     CDS             2800..5055
                     /locus_tag="PKU75_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80158.1"
                     /translation="MSSPPASASATPPAPPARGVSAWARLRAWVTAEPEYAAQPDFTR
                     QLTRAGALGLLLSLLVSLTLLPRSAQYFPGRVMGISLAVFAVATLVAVTQAVRNPERL
                     GGLRLAGVTSYLLLGVYAVWGDPLAPTTAYAPVTTPLMIGAAAFTVGFGPRRGAAASI
                     LLGLAVAHQRALAVGVGQGALEAVMFTGTQMAGAVLIQALLDESRAVIAGYRSLTEMT
                     MREKLANADLAAGSYLDSLVHNHVLATLLTASVASSPAEHDQAAAQARRALALMNRPS
                     PLRRPQEWRDLVQEQADRLGLTLHIAVAGTPRDPASAQAILDATTAALTNVARHSGQR
                     EVHVQASFTPRRSTVTVRDEGAGFLPDRISSRRHGVRHGIVARIEDLGGTVAIDSRPG
                     AGTVIRMSLSKTPSVLPVVEFPPVVRNVLFFTICLFVQAAFLAIGWRFREAYPWWLMP
                     LVVLTFGVGLTVLWTIPATSRAWIPVAAAAVVVPTLALAVTDDVTTGDWRLWFVGFFD
                     PVFATVAFRFSARAATLLALAQFPLYVGVIRLTAGSWAPAQAIEATVQLVLWAVVGGH
                     LAWAFSSSTERTLALAAATRRRDDAARLAQANTEALDVRLADLRTRAKGLLEHIAAAA
                     GDDAGLADRARVAEAGLRDRLRFPALVDGETLDALGAARARGVQVSLHVDDRAPSIGP
                     ALRTAIAAVLEAAEPGDQVTVTAYPTSGAARIVVANPHRAVPVPRCLEVAPVLRLVDH
                     DDGLLVLDVVE"
     gene            complement(5075..5740)
                     /locus_tag="PKU75_04450"
     CDS             complement(5075..5740)
                     /locus_tag="PKU75_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF80159.1"
                     /translation="MTAQLVARLAVVDDHPVVAEGIRRILEEHAPGVTWVGVATSYAG
                     LLDALREWDPLPDVVLYDLDLRDGSTTEDGIAQLHSRGVTVVICTGEFRPIPIRDAVH
                     AGAMGVMLKGDPPERMVDVISSAAAGDFAVSGDVAHLLISDEALTPKLAPREIEVLQL
                     LASGIPRKVIGKRMSPPVAETTVITYFKRAVQKYRDLGRQVETPQDAVRAAVHDGFLR
                     PIE"
     gene            5812..6087
                     /locus_tag="PKU75_04455"
     CDS             5812..6087
                     /locus_tag="PKU75_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HPF80160.1"
                     /translation="MCRNIRPLNNFEPPATSDEVHAAALQFVRKVAGTTKPSAANQEV
                     FDRAVAEIAHVTAHLLDDLVTTAPPKNREEEAAKARARAAVRYGRPA"
     gene            6071..6781
                     /locus_tag="PKU75_04460"
     CDS             6071..6781
                     /locus_tag="PKU75_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPF80161.1"
                     /translation="MAVPPDPPIVAAVRSGLAPHARAANAVAQQRYMKSAMPYRGITS
                     PDLKQILRGIFAAGPLPASRAEWERAVLALYDGASFREERYAALALAAHRSARGWQDA
                     STLPLYRHLISTGAWWDLVDTCTSSCVSPILLAHRDLATPVMREWSVADDMWLRRASI
                     ISQLPHKAGTDRSLLTEVIEANVEGTAYGSQFFIRKAIGWALRQYARIDPQWVRGFVD
                     THAEQLSGLSVREATKHL"
     gene            6944..9049
                     /locus_tag="PKU75_04465"
     CDS             6944..9049
                     /locus_tag="PKU75_04465"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HPF80162.1"
                     /translation="MPQTPKKSTAKGTAKREVPAPRDRAGNGGETHQLPVGDEVMTTA
                     QGAPLSDGQNSLRAGDRGPTLLEDFALRDKIFHFDHERIPERVVHARGVGAHGYLETL
                     EAIPELTRAALFQEKGTRTEAFVRFSTVAGNLGSADLARDVRGFAVKLYTSEGNWDLV
                     GNNIPVFFIQDATKFPDLVHSVKEEQDRGWPQAASAHDTFWDFISLMPEATHMVMWTM
                     SDRAIPRSLRFMEGFGVHSFRMVNAAGESRFVKFHWKPRQGMQSVLWDEAVKINGADP
                     DFHRRDLHAAITKGDLPQWDLGIQVFDDAFADQFDFDILDPTKLIPEEILPVRVIARL
                     TLDRVVDNIFAETEQVAFCTQNIVPGIDFSNDPLLQGRNFSYLDTQLSRLGSTNFTHI
                     PVNAPRCPVAHFQRDGHMQTMPQAGRANYEPNSFTGADRGPRADPDSGFVSAPVPESG
                     DKRRIRAESFADHYSQANQFWRSQTPVEQEHIVGGFAFELGKCEIEGIRTRMLANLVN
                     VDPDLATRVATKLGMDLPEASESAVPPRHDLPESPALSILKSTPQIFAGRKIGVVVTD
                     GTPTESLAGLRKAAEAAGATVELIGPTAGGVQLGRRKVPVDHAIAGAPSVLFDAVALL
                     PSADGAAELAKRKCAKDFVADAFGHQKFVAHSSDAQPLLTAAGVEADGGVFDLGDRSA
                     DEFVDACTALRFWERDVQP"
     gene            9098..9730
                     /gene="idi"
                     /locus_tag="PKU75_04470"
     CDS             9098..9730
                     /gene="idi"
                     /locus_tag="PKU75_04470"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HPF80163.1"
                     /translation="MTVSTGPSGRPAPEELVVLVDESGHATGTAAKTEIHDEATPLHL
                     AFSCYVFDAAGRLLVTTRARSKRTFPGVVTNTVCGHPGPGESLDTAVRRRADAELGMT
                     LGAVRLVLPRFRYRAEMAGVVENELCPVLIATAEESAGTTAEVTPDHREVEAVEWVPW
                     RDFVAEVSQGHRQVSPWCALQVAQLDRMGPDPAAWPAGDPSLLPPAARLA"
     gene            complement(9863..10660)
                     /locus_tag="PKU75_04475"
     CDS             complement(9863..10660)
                     /locus_tag="PKU75_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPF80164.1"
                     /translation="MFSLAWVLLGSCHPVPTLIVSALTGLLSQAMGLGTGATILVILA
                     VLTGQLSIGWSNDVFDADRDAAAGRGDKPLASDHGHRPVVRAAAALALAATIALSLAL
                     GARAGLTHLVFGVASGWAYNAWLKATMWSWLPFLLAFGTLPQVVALTAGRLAPAWVSV
                     TAGLLGVAAHAVNVLPDIADDRLTGLRGLGPRLGERGVRLLATAALIAAITTVLLSAD
                     LPTGTALATAAAAAGGGVAALFGSGKVPFLALGSLALVVAALMLSYL"
     gene            10661..11803
                     /locus_tag="PKU75_04480"
     CDS             10661..11803
                     /locus_tag="PKU75_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HPF80165.1"
                     /translation="MHTVWPRASRILAAHGVVPEHKYHQGDLTAGFAQIAGLTGARAQ
                     LLRRLHANSGVQTRHLAMPLAELTALRTFGEANDAFIEHGVALGARALSEAAETAGIP
                     LDRIDLIVSATVTGLAVPSLDARIASLLGLRPDVRRMPLVGLGCVAGAAGIARVHDYL
                     LGHPGHVAAFVSVELCSLTVQRDDASTANLVASGLFGDGAACVLMAGAEAPGAGSGAA
                     ETAGAAGTAKTTGRPRPSLAVLGTRSRLYPDSARLMGWDVRETGLRIVLGADVPDLVE
                     ANVRADVDEFLAGHEMVRGDVAFWVAHPGGPKVLEALHSSLDVDREALRLTWNSLERV
                     GNLSSASVLHVLADTLRERPPAAGSRGVLLAMGPGFCLECVLLEAR"
     gene            11800..12348
                     /locus_tag="PKU75_04485"
     CDS             11800..12348
                     /locus_tag="PKU75_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPF80166.1"
                     /translation="MTPVLFTVIVALVGLERLAELVVSNRNLAWARERGGQEYGAGHY
                     PAMVLLHTGFLAAMLAEVWIMRPPAPAWWGVALAGAVSAQALRWWCIRTLGHRWNTRV
                     VVVPGLPLVDSGPYRWFAHPNYVAVVLEGVALPLIHGAWWTAVGFTLLNAVLLRTRLR
                     VENAALQQAPSSPGARPAARGQ"
     gene            12359..13426
                     /locus_tag="PKU75_04490"
     CDS             12359..13426
                     /locus_tag="PKU75_04490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF80167.1"
                     /translation="MKDVIVIGGGPVGLVAAIHAARRGLSVTVLEPRGAPIDKACGEG
                     IMPHGVRELDAIGVRPPGMPIHGIVYADERRSVRAPMRSGPGLGVRRTALHLALTEAA
                     RGAGVETLAAASSGVAIHEGRVEVSLGRTGELLRSRYVVAADGLHSPTRRGLGVDRPV
                     GLRHKRFGLVTHARTSEVPTDVEVHWARDCELYVTPVSEDVVGLAVLSEARAPLSAKL
                     PEFPSVLARVDRGSVEDRVLGAGPFRQRSARRVVGGRVLLVGDAAGYVDALTGEGLSL
                     GFAQARLAIDSLVSGRVQGYEKAWRRTVREPTALTGGVLLASRWGPVRSGIVPAAVAL
                     PTVFEAIVNRLAGAPAHPSAR"
     gene            complement(13383..14066)
                     /locus_tag="PKU75_04495"
     CDS             complement(13383..14066)
                     /locus_tag="PKU75_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HPF80168.1"
                     /translation="MNPAFSRYVGKLTPRTPESGGGDRERIPAQRGAYVRLETGDQLT
                     LIDPVGEQVSDLYLVDVEDVEDHFSAGRTTDYGNTIYPSTGTILYSSTSRRLAAVTRD
                     TVGVHDMTLTPCSQDTFDILYPHLGGAAHPSCHANLVAALAPVGVDARHIGTTLNVFM
                     NVWTDPEGELHIDPPPTVAGDLFTIRALRPIHVGITACSAEGSNNGHCTPIDYLIERS
                     DAPAPRPAG"
     gene            complement(14123..14596)
                     /locus_tag="PKU75_04500"
     CDS             complement(14123..14596)
                     /locus_tag="PKU75_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPF80169.1"
                     /translation="MSDRETIAEVMKATRIAILTYEDEQGRLVSTPMGTQDFDDPATV
                     WFITEADSDKVAAISRQPRVNVSYASDKGWVSLSGTASLNQDRAKLEELWDPSASAFM
                     QGGPDDPNSALLEVSGDTAQLWESPGKLGMLVQVAKGALGKEDPAKDSDAPVVDL"
     gene            14955..16283
                     /locus_tag="PKU75_04505"
     CDS             14955..16283
                     /locus_tag="PKU75_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPF80170.1"
                     /translation="MESDVSPSRQVLQLAIPAFLALIAEPLFLLADTAIVGHLGTAPL
                     AGLGLASSALLTAVGVFVFLAYGTTAVVARQLGAGNVRAAIAAGVDGTWLAVGLGLVV
                     AAPLALAARPVCRWLGGEGAALTNAVGYLRIAAFGIPPMLVVMAVTGVLRGLLDTRTP
                     LVVNVVGAVVNVVLNVLFVYGFRWGIAGSAIGTLLVQAGMASALVLVLLRHARADDAP
                     LRPHPGRVLAAAIDGISLLVRTLALRAILLLTTWVAAGLGVVPLAAHQVTATIWSFLV
                     FALDAIAIAAQALTGRALGAGDARSARELTSLMIRWGIWSGLAVGVVVLALHRVLPAL
                     FTDDPGVRAAVAAGLVVVAVGQVLSGFVFVIDGVLIGAGDARWLSMSMCLVLAAYLPM
                     VLAVRAFAPTDPPLAITWLWVAFTGFMLVRAVFLGWRVRSDRWMVLGAGR"
     gene            complement(16721..18247)
                     /locus_tag="PKU75_04510"
     CDS             complement(16721..18247)
                     /locus_tag="PKU75_04510"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPF80171.1"
                     /translation="MTDHVAVAATGPAAVDAGLTAVRAGGNAVDAAIAAMCVAMTTEP
                     GVVSPMGGAFVTIWPAGGEPETIDGNVEMPGRGLDPARFGQGLREFHLNYGGGITVFG
                     GYGSVAIPGALSALQLAHDRHGSGAWAQAIAPAEKAAREGFRLGSAAASYLALTVDNL
                     FGWDADTHQALTREDGSALEPGDLLIDTDMADSWARIAEHGAADVYTGEIGRRIAAAC
                     TAEGGLITAADLAAYRPVVREPLRQRLGAWDIATNPPPSVGGPMLAVMLSEMARRGSL
                     SWVDTIEVQRHVLTYRSEVHDFSADLEEDGYNMLEEVRRAGVAGLTTSQSTAHVSAVD
                     SDGLGCAITASSGYSSGATVPGTGLLLNNCLGEPELNRLGLHVLAPGTRLASNMAPSV
                     AHHDSGDVLAIGSPGADRITTALMQVLARHCLIGMPLGEAIAAPRAHVRVVRGEPTRV
                     EFERDDDIRAAIAACGLPANEHEPHAMFFGGVGGAARSATGVLFAAGDPRREAATGVA
                     "
     gene            18346..18534
                     /locus_tag="PKU75_04515"
     CDS             18346..18534
                     /locus_tag="PKU75_04515"
                     /inference="COORDINATES: protein motif:HMM:NF017530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HPF80172.1"
                     /translation="MLLGEEVLLLVTDDTTGRAPALSQHLDVVIGGSLFAELTLRERI
                     TLTPHELGWRARDRVQVL"
     gene            complement(19083..19646)
                     /gene="thpR"
                     /locus_tag="PKU75_04520"
     CDS             complement(19083..19646)
                     /gene="thpR"
                     /locus_tag="PKU75_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPF80173.1"
                     /translation="MRLFVAIIPPVEVVENLTDFLRPRRDAGAELRWTDPEHLHLTLA
                     FLPDVGEAGLDRLTEALTERCAALPAAAMRLSGGNAFPNPYAARVLFAAAEPEPWLGK
                     VARAARNAAGHAGVVVDGGRFHPHVTVARLRRPTEATRWIRILQGYESPGWVAESACL
                     VQSRLGQGRQGRPLYDVLAEFPFAPAP"
     gene            complement(19797..21527)
                     /locus_tag="PKU75_04525"
     CDS             complement(19797..21527)
                     /locus_tag="PKU75_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPF80174.1"
                     /translation="MQRTRFYAAGAAAAIAASGLAVAATAGTASAAPTAERAAAGSYV
                     VLAREGADAQAIADRLAAGGARITSVNTDIGLISLDTTSSTFVDDTSRMDGVQGVARD
                     RAIGRSPGVAPRPSDVEKENRVTPAAERGTSDSDRAGAKYSGDPLDRWLWGMDMIKAG
                     AAHQKTLGDKRVRVGILDTGVDGRHPDLAANFDRRLSRNFTTDMTDVDGECEYTSCKD
                     PNNVDDNGHGTHVAGTIAAAMNGMGLSGVAPKATIVNIRGGQDSGYFFLGPVSNALTY
                     AANTGLDVVNMSFYVDPWLYNCTGGAPEDTPAQAREQETVIATMNRALKYATSKNVTL
                     VAALGNQHDDLAKPRQDVSSPDYGAEPHRRTIFNSRCVTLPTEGPNVLGISSLGPSGT
                     KSDFSNYTTEVYSTEIEFSAPGGWYRDGFGTASYRTNENMILSSAPRRVLQAEGAVDP
                     KGNITPLGKELGVKKACTANPAPGADRCGFYQYLQGTSMAAPHATGVAALAVSAHGHK
                     SADGLTMKPGAVRWLMAQTATDHACPAGGVQEYVNEDRDETYTAICRGTTDRNGFNGF
                     GIVNANGVVR"
BASE COUNT         3047 a         7659 c         7607 g         3330 t
ORIGIN      
        1 cgcatcatcg cggtcatcaa cgaccagatg ccctacacct acggcgacgc ccaggtgccg
       61 gtcgacctgg tcgactacgc catcgaggtc agcgtcccgc tgcccgagat caccccggcc
      121 acccccgatg ccgacagcaa ccgcatcggc gaactcgtcg ccgcccgcat acccgccggc
      181 gccaccctgc aggccggcat cggcgccatc cccgacgcca ccatcgctca actgcatacc
      241 cacaagggcc tgcggatctg gaccgaaacc ttctccgacg gcgtcctggc cctcgaacgc
      301 ggcggatcca tggatcccaa ccacccgctc accaccagct tcctgatggg cactcgcgaa
      361 ctctacgact gggtcgacgg caacccgcgc gtgcggatga tgcgcaccga gcgcaccaac
      421 gacccggccc tgatcgccct ccagcggcac atgacctcga tcaacaccgc cctcgagatc
      481 gacctgttcg ggcaggccaa cgccagccgc atcaacgcgc ggatctattc cggcttcggc
      541 ggccagaccg acttcatcgt cggcgccctc cattccccgg ggggcgcggc atacatggcg
      601 ctgcgctcct ggcaccccaa ggcgaacgtg tccacgatcg tccccctcgt cgacgagccg
      661 gtcacctcct tccagcagtc ggccgtcgtc accgagaacg gcgtcgccga actgtggggt
      721 tatacccagc aggcccaggc ccgccacctg atccgcgacg ccgcccaccc cgacgtgcgc
      781 gaggagctct gggaggaagc ccgcgaactc ggcctcgcct gatcgcgcac acaagcaatc
      841 acgcacacac gtaatcgcgc acgccggaac cgatccgccg cccctgcgct ctaaggggag
      901 tcgcgcgcgc cgccggaggg gaagcgagcg cggcacgatt gcttccccgt gccgtatgcg
      961 gtcgccgtgc cgtatgccgt cgcgatgacg gtatgccgtc gcccacccgt ccgggaccgg
     1021 acgcggcggc ctttcttacc tggtacgccg gggaccgaca tcactccctc gtaacggctt
     1081 tgctccaatg atccacttca catgccgttc aggcggtttg ggaggcaata tctaaggtgt
     1141 gagcacgtcg gccccggagg acttcgcgca ggcgctcgcc cagcgtctcg gtgggcaatc
     1201 ggtccggcag ctcgcggcgg tttcgggatg gggccggacc acggtttccg acatccgcgc
     1261 gggccggcac atcccgagcg aggcacagtt gcgcgacctg ctcatgtcgg tcggcgccga
     1321 cgagtcggag atcgccagct ggcaggagcg ccgctcggcc atcatcaccc cgccggcggc
     1381 gccggccgcg cctcccacac tgcccgtcac gcctcccgca ccgcccgtca ccccgccgcc
     1441 cgttccgcgg cgccgtcttg cgcgggccgc ggtcgccgcc gccggtgcga tctccctgct
     1501 ggggatcggc ttcgccggag ggtatgccgc acgcgaccgg gcaggcgcgc acgcccagca
     1561 ggtctcggcg gcagcgaacc cgggccggat ctccggaacg cgcagcgccg tggtcgaagt
     1621 cagccagggc gcctcccacc tgtgcgttcg cacggcgggc ggggccatcg agtgcatggg
     1681 ccgcaacaag gtcggtcagc tcggggacgg gttgcgcgtc gactccgcgg ttccggtgcc
     1741 cgtcggcggt ctgctcgccg gtgccaaacg gatctccgcc ggcggcgatt tcgcctgcgt
     1801 catcaacttc gacgaccagg ccaaatgctg gggccacaac gcctacgggc aggcgggcaa
     1861 cggccaggag acgatgctcc tcaccccgac gctggtcgag gatctccccg acgtcaagga
     1921 gattgccgtc ggcaacctgc acgcctgcgc cctcaccctg gccggcgacg cctactgctg
     1981 gggcaacggc gagcacggcc agctcggcat cggcaggttc ctgcgcaacg ccgcccggcc
     2041 cgtgctcgtc cagggcatcg aggccgaact tgccggcatc acagccgggc gtgatttcac
     2101 ctgcgcctgg tcgacccgct ccgaggtcta ttgctggggc gccggttcat acggtcagct
     2161 cggcatcggc acccgccagg acgagccgac ccctgtccgg ctcgagaagg cctacccctt
     2221 gggagtgcgc gccatggctg cggggtatga ccacgtctgt gccatcgcgc cgggtggcgg
     2281 cgtcgactgc tggggtcgtg gcgacagcgg ccagctcggg accgggtcga ccgaggatgc
     2341 cctgactccc cggccggtgg tgggactcga cgcagccgag gccctggccg cgagcatccg
     2401 gcatacctgc gccgtgacac cgaccggcgc ggtggcgtgc tggggcgacg gcgcatccgg
     2461 gcagctcggg ccgcagagcg acggctcggc caggccggtc atcgtccccg gtgtcagtgg
     2521 catccgcaag gtgagcgccg gccagcactc gagttgtggc atcagcgccg atgcgctctg
     2581 gtgctggggc gcgagccgct acggcgagat cggcgcaggc agccgcgaga acctcgccca
     2641 gccgcaccgc gtcgcgatca gctgacacgg cgctgacctg cgctttaggg tgtccgctgc
     2701 ccgaccggac acccccggac gccgatcggc cgcgcggacg ccgagggctt tggcatcctc
     2761 cgaagtgagc tccccccacc ccacccccgc cttgactcca tgtcatctcc gcctgcctcc
     2821 gcgtccgcca ccccgcccgc cccgccggct cggggcgttt cggcgtgggc acggttgcgc
     2881 gcgtgggtga cggccgagcc ggagtatgcc gcgcagccgg acttcacccg ccagctcacc
     2941 cgggccggcg ctctcgggtt gctgctgtcg ctgctcgtct cgctgaccct gctgccgcgc
     3001 tcggcgcagt acttcccggg ccgggtcatg ggcatcagcc tggccgtctt cgccgtcgcc
     3061 acgctcgtcg ccgtcaccca ggccgtccgc aacccggagc gcctcggcgg cctgcgcctg
     3121 gccggcgtca cgtcatacct cctcctcggc gtatacgcgg tgtggggcga cccgctggcg
     3181 ccgaccacgg catacgcacc ggtgacgacc cccctgatga tcggggccgc cgccttcacg
     3241 gtcgggttcg ggccacgtcg cggcgccgcg gcctcgatcc tcctcgggct cgccgtggcc
     3301 caccagcgcg ccctggcggt cggggtgggt cagggcgcgc tggaagcggt catgttcacg
     3361 ggcacccaga tggcgggtgc cgtgctcatc caggcgctgc tcgacgagag ccgggcggtg
     3421 atcgccggct accggtcgct gaccgagatg acgatgcgcg agaagctggc caacgccgac
     3481 ctcgctgccg ggtcctatct ggacagcctc gtccacaacc acgtgctggc caccttgctg
     3541 accgccagcg tggccagttc accggccgag cacgaccagg ccgccgccca ggcccgccgc
     3601 gccctcgcgc tgatgaaccg tccctccccg ttgcgacgac cccaggaatg gcgtgacctg
     3661 gtgcaggagc aggccgaccg gctgggtctg accctgcaca tcgccgtcgc cgggacaccc
     3721 cgggacccgg ccagcgccca ggcaatcctc gatgcgacga ccgcggcgct gaccaatgtg
     3781 gcccgccact ccggccagcg cgaggtgcac gtgcaggcga gcttcacgcc ccggcgcagc
     3841 acggtgaccg tgcgcgacga gggcgcgggc ttcctcccgg accggatttc ctcgcgacgg
     3901 cacggagtcc gccacggaat cgtcgcgcgc atcgaggacc tcggcggcac ggtcgcgatc
     3961 gacagccggc cgggcgccgg cacagtcatc cgcatgtcgc tgtccaagac gccgtcggtt
     4021 ctgcccgtcg tggagttccc tccggtggtc cgcaacgtcc tcttcttcac gatctgcctg
     4081 ttcgtccagg ccgcctttct cgccatcggg tggcgcttcc gagaggccta tccctggtgg
     4141 ctcatgcctc tggtcgtcct cacgttcggc gtcggcctca ccgtgctgtg gacgatcccg
     4201 gcgacctccc gcgcctggat ccccgtggcc gccgcggccg tggtcgtgcc cactctcgcg
     4261 ctcgccgtca ccgacgatgt cacgaccggt gactggcgtc tgtggttcgt cggattcttc
     4321 gaccccgtct tcgccactgt ggccttccgc ttcagcgccc gcgccgccac gctcctggcg
     4381 ctggcccagt tcccgctcta cgtcggcgtc atccggctga ccgccggcag ctgggcgccc
     4441 gcccaggcca tcgaagccac cgtgcagctg gtgctgtggg ccgtggtggg aggccacctc
     4501 gcgtgggcct tctcctccag caccgaacgg accctcgcgc tggccgccgc gacccggcgg
     4561 cgtgacgacg cggcccgcct ggcgcaggcg aacaccgaag ccctcgacgt ccggctcgcg
     4621 gatctgcgta cccgcgccaa aggcctgctc gaacacatcg ccgccgccgc cggcgacgac
     4681 gccggcctgg cggaccgggc gcgcgtggcc gaggccgggc tgcgcgaccg gctgcgcttt
     4741 cctgccctcg tcgacggcga gaccctcgac gccctcggcg cggcccgggc ccggggtgtg
     4801 caggtcagcc tgcacgtcga cgaccgtgcc ccgtcgatcg gccccgccct gcggacggcg
     4861 atcgcggccg tgctcgaggc cgcggaaccg ggcgatcagg tgacggttac ggcatacccg
     4921 acatcgggcg cggcccgcat cgtcgtcgcc aacccgcacc gggcggtgcc ggtaccgagg
     4981 tgcctcgagg tcgcgccggt gctgcggctc gtcgaccacg acgacggact gctcgtcctc
     5041 gacgtcgtgg agtgatctct gcgcgccgtg ccgatcactc gatgggacgc aggaatccgt
     5101 cgtggacggc ggcgcgcacc gcgtcctgcg gggtctcgac ctgccggccg aggtcgcggt
     5161 acttctgcac cgcacgcttg aaataggtga tcaccgtcgt ctcggcgacc ggcggcgaca
     5221 tgcgtttgcc gatgaccttg cgggggatgc cgctggcgag cagttgcagc acctcgatct
     5281 cgcgaggcgc gagcttgggg gtgagcgcct cgtcggagat cagcagatgc gcgacgtccc
     5341 cgctgacggc gaaatcgccg gcggcggccg agctgatgac gtcgaccatc cgctccggcg
     5401 ggtcgccctt gagcatgacg cccatcgcgc cggcgtggac ggcgtcgcgg atcgggatgg
     5461 gccggaactc gcccgtgcag atcacgacgg tgacgccccg gctgtgcagt tgcgcgatgc
     5521 cgtcctcggt ggtggagccg tcgcgcaggt ccaggtcgta gagcacgacg tcgggcagcg
     5581 gatcccactc ccggagagcg tcgaggagcc ccgcgtatga cgtggccacc ccgacccacg
     5641 tcacgccggg ggcgtgctct tcgagaatgc gccggatgcc ctccgcgacg accgggtggt
     5701 cgtcgacgac ggcaagccgg gccaccaact gcgctgtcat gcgtccatga tgctccccgc
     5761 gggtaatgcc gcacctgtcg cccattggga gggccgacct acgatcgtca catgtgtcgc
     5821 aacatccggc ccctgaacaa cttcgagcca cccgccacgt cggatgaggt gcacgccgcg
     5881 gcgttgcagt tcgtgcgcaa ggtggccggg acgaccaagc cgtccgccgc caaccaggag
     5941 gtcttcgacc gcgcagtcgc cgagatcgcg cacgtcaccg cgcacctgct cgacgatctc
     6001 gtcaccacgg cgccgccgaa gaaccgcgag gaggaggccg cgaaggcgcg cgctcgcgcg
     6061 gcggtgcggt atggccgtcc cgcctgaccc gccgatcgtc gccgccgtcc ggtccggact
     6121 cgcgccgcac gcgcgagccg ccaatgccgt tgcgcagcaa cggtatatga agtccgcgat
     6181 gccctatcgg ggcatcacga gcccggacct gaagcagatc ctgcggggca tcttcgccgc
     6241 cgggccgctg ccggcgtcgc gggcggagtg ggagcgggcg gtgctggcgc tgtatgacgg
     6301 ggcgagcttc cgcgaggagc ggtatgccgc gctcgccctg gccgcgcacc gctcggctcg
     6361 cgggtggcag gacgcgtcca cgctcccgct ctatcggcac ctgatctcca ccggcgcgtg
     6421 gtgggacctc gtcgacacct gcacctcctc gtgcgtgtcg ccgatcctgt tggcgcacag
     6481 ggatcttgcg actcctgtca tgcgggagtg gtcggtggcg gacgacatgt ggctgcggcg
     6541 agcgagcatc atcagccagc tgccccacaa ggcgggcacc gaccggtcct tgctgaccga
     6601 ggtgatcgag gcgaacgtgg agggcacggc atacggctcg cagttcttca tccgcaaggc
     6661 gatcggttgg gcgctgcggc agtatgcgcg catcgatccg cagtgggtgc gcggcttcgt
     6721 cgacacccat gccgagcagt tgtcgggact cagtgtgcgc gaggcgacga agcacctgtg
     6781 agccggccag ccacccttag gggtggtttc ctctccgcgg ccgtcctggg ccggtgctta
     6841 gatctggtgc gcgcaccgtc gcgctatagc gtgaaaactg ccgcccgtga cccggtcgcc
     6901 cgtgcggcac acctccatct cgggccggtc atcaggagag gtcatgccac agacgcccaa
     6961 gaagtcaacc gccaagggga cggcaaagcg agaggtcccc gcgccacggg atcgcgccgg
     7021 caacggtggc gaaacgcacc agctgccggt cggcgacgag gtcatgacga ccgcgcaggg
     7081 tgcaccgctc tccgacggcc agaactccct gcgcgccggc gatcgcggac cgacgctgct
     7141 ggaggatttt gcgctgcgcg acaagatctt tcacttcgac cacgagcgca tccccgagcg
     7201 ggtcgtgcac gcgcgtgggg tgggggcgca cggctatctc gagaccctgg aagccattcc
     7261 ggagctgacg cgcgctgccc tcttccagga gaagggcacc cgcaccgagg cgttcgtgcg
     7321 gttctcgacc gtcgccggca acctcggctc cgccgacctg gcgcgcgatg tgcgcggttt
     7381 cgcggtcaag ctctacacca gcgaaggcaa ctgggacctg gtgggcaaca acatcccggt
     7441 cttcttcatc caggacgcga cgaagttccc cgacctggtg cactcggtca aggaggagca
     7501 ggaccgcggc tggccgcagg ccgcgagcgc ccacgacacc ttctgggact tcatctccct
     7561 gatgcccgag gcgacccaca tggtcatgtg gaccatgtcg gaccgggcga tcccgcggtc
     7621 gctgcggttc atggagggat tcggcgtcca ctccttccgg atggtcaacg ccgccggcga
     7681 gtcgaggttc gtgaagttcc actggaagcc gcggcagggg atgcagtcgg tgctctggga
     7741 cgaggcggtc aagatcaacg gcgccgaccc cgacttccac cggcgggatc tgcacgcggc
     7801 catcaccaag ggcgatctcc cgcaatggga tctgggcatc caggtcttcg acgacgcctt
     7861 cgccgaccag ttcgacttcg acatcctcga ccccaccaag ctgatccccg aggagattct
     7921 gccggtgcgc gtcatcgccc ggctcaccct ggaccgggtc gtcgacaaca tcttcgccga
     7981 gaccgaacag gtcgccttct gcacccagaa catcgtgccg gggatcgact tcagcaacga
     8041 cccgctgttg cagggccgca acttctccta cctcgacacg cagctctccc ggctcgggag
     8101 caccaacttc acccacatcc cggtcaatgc accccgctgc ccggtcgcgc acttccagcg
     8161 cgacggacac atgcagacga tgccgcaggc cggccgggcg aactacgagc cgaactcgtt
     8221 caccggggcc gaccgcgggc cgcgggcaga tccggactcg ggtttcgtct ccgcgccggt
     8281 gccggagagt ggggacaagc ggcggatccg cgccgagtcg ttcgccgacc actacagcca
     8341 ggcgaatcag ttctggcgca gtcagactcc cgtcgagcag gagcacatcg tcggcggttt
     8401 cgccttcgag ctcggcaagt gtgagatcga gggcatccgc acccggatgc tcgccaacct
     8461 cgtcaacgtc gacccggacc tggcgacgcg ggtcgcgacg aaactgggca tggatctccc
     8521 ggaggcgtcc gagagcgcgg tgcccccgcg gcacgacctg cccgagtcgc ccgccctgtc
     8581 gatcctcaag agcacgccgc agatcttcgc cggacgcaag atcggagtcg tcgtcaccga
     8641 cggcacgccg accgagtcgt tggcggggct gcgcaaggcc gcggaagcgg ccggggcgac
     8701 cgtcgaactc atcggcccga cggccggcgg cgtccagttg ggacgacgca aggtcccggt
     8761 cgaccacgcc atcgccggcg caccgtcggt gctgttcgac gccgtggcgc tgctgccgag
     8821 cgccgacgga gccgcggagt tggcaaaacg caagtgcgcc aaggattttg tggccgacgc
     8881 gttcggccac cagaagttcg tggcccactc gtccgacgct cagccgttgt tgactgctgc
     8941 cggcgtcgag gctgacggcg gcgtgttcga cctcggtgac cggtccgcgg acgagttcgt
     9001 cgacgcctgc accgctttgc ggttctggga gcgcgacgtc cagccctgat cacgcctcgc
     9061 gggcgtggct gagctccacg tggtcgatga tgggccggtg actgtcagca ccggcccatc
     9121 gggtcgcccg gcgcccgagg agctcgtggt cctggtggac gaatccggtc acgccacggg
     9181 caccgccgcg aagaccgaga tccacgacga ggcgacgcct ctgcatctgg ccttctcctg
     9241 ctacgttttc gacgccgcgg gccggctgct ggtgacgaca cgggcgcgca gcaagcgcac
     9301 cttcccggga gtcgtgacga acaccgtatg cggtcacccc gggcctgggg agtcgctcga
     9361 cacggccgtg cggcggcgcg ctgacgccga gctgggtatg acgctcggcg cggtccgcct
     9421 ggtcctgccg cgctttcgct atcgcgccga gatggccggg gtcgtggaga acgagttgtg
     9481 ccccgtcctc atcgccacgg cggaggagtc ggcgggtacg accgcggagg tgacgccgga
     9541 ccaccgggag gtcgaggcgg tcgagtgggt gccgtggcgc gacttcgtcg ccgaggtcag
     9601 tcaggggcat cggcaggtgt cgccgtggtg cgcgctgcag gtggcgcaac tcgatcgaat
     9661 gggaccggat ccggcggcgt ggcccgccgg cgacccgtcg ctcctgccgc ccgccgcccg
     9721 cctggcctag cgcgtgtatc gaaaaggacg tacaacgaag agccaccgac ggggtggggt
     9781 ccccgcgaag taccggagcg acgacggagt tgggtcccac gctctgcgtg ggcggacacg
     9841 tcgtgggggt ggccgagcgg cctcagagat agctcagcat gagcgcggca acgacgaggg
     9901 cgagactgcc cagcgccagg aacgggacct tgccggatcc gaagagggcc gcgacgccgc
     9961 caccggcggc ggccgcagcg gtcgccaagg cggtccctgt gggcaggtct gcgctgagca
    10021 gcaccgtcgt gattgcggcg atcagggccg ccgtggcgag caggcgcacg ccccgctcac
    10081 ccagtcgggg tcccaggccc cggagcccgg tgagccggtc gtccgcgatg tccggcagca
    10141 cgttcacggc gtgcgcggcg acgccgagca ggccggccgt cacgctcacc cacgccggcg
    10201 cgagcctgcc cgcagtgagg gccaccacct gcggcagcgt cccgaacgcc aggaggaacg
    10261 gcagccacga ccacatcgtc gccttcaacc acgcgttgta ggcccagccg gacgccacgc
    10321 cgaacacgag gtgggtcagt ccggctcgag ccccgagcgc cagcgaaagg gcgatcgtgg
    10381 cggccagggc cagcgcggca gccgcccgca cgaccggccg gtggccatgg tcgctcgcca
    10441 acggtttgtc gccgcgaccc gccgccgcat cgcgatcggc gtcgaagacg tcgttggacc
    10501 agccgatcga caactgcccg gtgagcaccg cgagaatcac gagaatcgtc gccccggtcc
    10561 ccagccccat cgcctgtgac aaaagtccgg tcagcgccga aacgatcagc gtgggaaccg
    10621 gatggcatga tccgaggagc acccaagcga gggagaacac gtgcataccg tatggccgcg
    10681 agccagccgc atccttgcgg cgcacggggt ggtcccggag cacaaatacc accaaggtga
    10741 cctgacggcg ggcttcgcgc agatcgccgg gctgaccgga gcgcgggcgc aactgttgcg
    10801 gcgcctgcac gccaacagcg gtgtccagac ccggcacctg gccatgccgc tggcggagct
    10861 gaccgcgttg cggaccttcg gcgaggccaa cgacgccttc atcgagcacg gggtcgcgct
    10921 cggcgcccgg gcgttgtccg aggccgccga gacagccggc ataccgctcg accggatcga
    10981 cctcatcgtg agcgcgacgg tcaccggcct cgccgtgccc tccctcgacg cccgcatcgc
    11041 cagtctgctg gggctgcgtc cggacgtgcg tcgtatgccg ctcgtcgggc tcggatgcgt
    11101 cgccggcgcc gcggggatcg cgcgggtgca cgactatctg ctgggccatc cgggccacgt
    11161 cgcggcgttc gtatccgtgg agttgtgcag cctgaccgtg cagcgcgacg acgcctcgac
    11221 ggccaacctg gtggcgtccg gactcttcgg ggacggcgcc gcgtgcgtcc tcatggccgg
    11281 ggcggaggca cccggtgccg ggagcggcgc ggcggagacg gccggcgcgg ccgggacggc
    11341 gaagacgacc ggccggccgc ggccgtcatt ggccgtcctc ggcacccgca gccgcctcta
    11401 tcccgacagc gcccgcctga tgggctggga cgtgcgggag acgggtctgc gcatcgtgct
    11461 gggcgccgac gtgcccgatc tggtggaggc caacgtccgg gccgacgtcg acgagttcct
    11521 cgccggccac gagatggtgc gtggcgacgt cgcgttctgg gtggcccacc cgggcggccc
    11581 gaaggtgctg gaggccctcc acagctcgct cgatgtcgac cgcgaggcgc tgcgcctgac
    11641 gtggaactca ctggagcgcg tggggaatct gtcgtcggcg tcggtcctgc acgtgctcgc
    11701 cgacaccctg cgggagcggc cgcctgcggc gggcagccgc ggcgtgctgc tggcgatggg
    11761 tccgggcttc tgcctcgagt gcgtgctcct ggaggccagg tgaccccggt cctcttcacg
    11821 gtgatcgtgg ccctggtcgg tcttgagcgc ctggccgaac tcgtggtgtc caaccgcaac
    11881 ctggcctggg cccgcgagcg cggcggccag gaatacggcg ccggtcacta cccggccatg
    11941 gtgctgctgc ataccggctt cttggcggca atgctcgccg aggtctggat catgcggccc
    12001 cccgcgccgg cgtggtgggg ggtcgcgttg gcgggcgcgg tatcggcaca ggcactgcgg
    12061 tggtggtgca tccgcacctt ggggcatcgt tggaacaccc gcgtcgtcgt cgtgccggga
    12121 ctgcccctcg tcgactccgg cccgtaccgt tggttcgccc accccaatta cgtggccgtc
    12181 gtgctcgagg gagtggcgct gccgctcatc catggcgcgt ggtggacggc cgtcggattc
    12241 acgctcctca acgcggtcct gttgcgcacg cgcctgcggg tcgagaacgc tgccctgcag
    12301 caggcgccgt cgtcgcccgg cgcccggccg gccgcccgcg gtcaatgacg ccacggcggt
    12361 gaaggacgtc atcgtcatcg gcggcgggcc ggtgggactg gtcgcggcga tccacgcggc
    12421 ccgtcgcggc ttgtccgtca ccgtgctcga gccgcggggc gccccgatcg acaaggcgtg
    12481 cggcgagggc atcatgccgc acggcgttcg ggaactcgac gccatcggcg tgcgaccacc
    12541 cggtatgccg atccacggca tcgtctatgc cgacgagcgc cgctcggtgc gggcaccgat
    12601 gcgttcgggt ccggggctcg gggtgcggcg cacggcgttg cacctggccc tcaccgaggc
    12661 tgctcggggg gccggtgtcg agaccctggc cgccgcctcg tcgggggtgg cgattcacga
    12721 gggccgggtt gaggtgagcc tggggcgaac cggggagctg ctgcggtccc gctatgtggt
    12781 cgcggcggac ggactgcact caccgacccg tcgtggcctg ggggtggacc ggccggttgg
    12841 attgcggcac aagcgtttcg gactggtcac gcacgcgagg acgtccgagg tgcccaccga
    12901 cgtcgaggtc cattgggcgc gcgactgcga gctctatgtc acccccgtgt ccgaggatgt
    12961 ggtgggtctg gccgtgctgt ccgaggcccg agccccgctg agtgccaaac tgccggaatt
    13021 tccgagtgtg ctggcacggg tggatcgagg cagcgtggag gaccgggtgc tgggcgccgg
    13081 gcctttccgg cagcggtccg cccggcgggt cgtcggcggc cgcgtgctac tcgtcgggga
    13141 cgccgccggt tatgtcgacg ccttgaccgg ggaaggattg tcgctcgggt tcgcccaggc
    13201 gcgcttggcg atcgactcgc tcgtttccgg acgggtgcag ggctacgaga aggcgtggcg
    13261 ccgcaccgtc cgcgagccca ccgcactgac cggcggggtg ctcctggcct ctcgctgggg
    13321 tccggtgcgg tccggcatcg tgcctgcagc cgtcgccctg cccaccgtgt tcgaggccat
    13381 cgtcaaccgg ctggccgggg cgccggcgca tccgagcgct cgatgaggta atcgatcggg
    13441 gtgcaatgtc cgttgttcga gccctcggcc gagcaggcgg tgatccccac gtggatcggc
    13501 cgcaatgccc tgatggtgaa caggtcgccc gcgaccgtgg gcggcgggtc gatgtgcaac
    13561 tcgccctcgg ggtcggtcca cacgttcatg aagacgttga gcgtggtgcc gatgtggcgc
    13621 gcgtcgacgc cgacaggcgc cagcgcggca accagattcg cgtgacacga ggggtgggct
    13681 gcgccgccca ggtgcgggta gaggatgtcg aaggtgtcct gactgcacgg cgtcagcgtc
    13741 atgtcgtgga ctccgaccgt gtcgcgcgtg acagccgcca atcgccggct cgtggagctg
    13801 tagaggatgg tgccggtgct gggatagatc gtgttgccgt agtcggtcgt gcgaccggcc
    13861 gagaagtgat cctcgacgtc ctcgacgtcc accaggtaga ggtcggagac ctgctcgccg
    13921 acggggtcga tgagcgtgag ctggtcaccc gtctcgagac ggacgtaggc accccgctgg
    13981 gcagggatcc gttcgcggtc ccctccgccg gactccggcg tccggggcgt cagcttcccg
    14041 acgtagcggc tgaaggctgg attcatggtt ctctctgttg tcccgatccc ggcgacgatc
    14101 gtcgtccgga tggcggagcg gtctagaggt cgaccaccgg tgcatctgag tccttggccg
    14161 ggtcttcctt gcccagcgcg cccttggcca cctgcacgag cattcccagc ttgcccgggg
    14221 actcccaaag ctgagcggtg tcgccgctaa cctcgagcaa cgcgctattg ggatcgtccg
    14281 gaccgccctg catgaacgcc gaggccgagg ggtcccacaa ctcctccagc tttgcgcgat
    14341 cctggttgag gctcgcagtc ccggagagcg agacccagcc cttgtcgctg gcatacgaca
    14401 cattgacgcg cggctgccgg gagatggccg cgaccttgtc gctgtcggcc tcggtgatga
    14461 accacaccgt cgccgggtcg tcgaagtcct gtgtgcccat gggcgtgctc accaagcgtc
    14521 cctgctcgtc ctcataggtc aaaatggcga tccgcgtggc cttcatgacc tcggcgatgg
    14581 tttctcgatc cgacatgaca gtcctttcat cgggcgaccc tcgcacggcc gcgcgtcagg
    14641 ttgtgtgcac gctaccgcgc gcgctcggca acgagactcg ttgtgggcca acgaatccac
    14701 gatcggtcgc cgttcgctgt cgaatcggcg gacgtggtcg gaatcccttc cctagggatg
    14761 taccgcgaag cgactcgagg tgctttggtg ggcaggacac ccgtgtccac cgcagcgacg
    14821 tcacgatggg gacgggacgg ccataccgtg tcggcacctc atgtcgtgcc gacccgcaga
    14881 ccacccgacc accagacctg ccgcgggcgt gggcaccccc gacacagtca tctcgatctg
    14941 ggcacactgg cgccgtggag tccgatgtgt cgccgagccg gcaggtgctg cagctggcga
    15001 tcccggcgtt cctcgcactg atcgcggagc cgctcttcct gctcgcggac acggcgatcg
    15061 tgggtcacct cgggacggcg ccgctggcgg gactggggct ggcaagttcg gcgctcctga
    15121 ccgcggtcgg tgtcttcgtc ttcctcgcct acggcacaac cgcggtcgtc gcccggcagt
    15181 tgggtgccgg caacgtgcgg gcggcgatcg ccgccggcgt cgacggcacc tggctcgccg
    15241 tggggttggg actggtcgtc gcggcgcctc tcgcgcttgc ggcgcggccg gtatgccggt
    15301 ggctcggcgg cgagggggcg gcgctcacca acgccgtcgg ctacttgcgg atcgccgcct
    15361 tcggcatccc gccgatgctc gtcgtcatgg ccgtcaccgg ggtgctgcgc ggactgctgg
    15421 acacgcgcac gccgctggtg gtcaatgttg tcggggccgt ggtcaatgtg gtcctcaacg
    15481 tgctcttcgt ctacggattc cgttggggca tagcgggttc ggcgatcggc acactcctcg
    15541 tccaggccgg tatggcgagt gccctcgtcc tcgtgctgct gcggcatgcc cgcgcggacg
    15601 acgctcccct acgcccccac ccggggaggg tgctcgcggc cgcgatcgac ggcatatcgc
    15661 tgctcgtgcg caccctggcg ctacgcgcca tcctcctgct gaccacctgg gtggccgcgg
    15721 gcctcggcgt ggtgccgctt gccgcccatc aggtgacggc gaccatatgg tccttcctcg
    15781 tcttcgcgct cgacgccatc gccatcgcgg cgcaggcgct caccggcagg gccctcggtg
    15841 cgggcgatgc gcgctcggcg cgggagctga cctcgctgat gatccgatgg ggtatctgga
    15901 gcgggctggc tgtcggtgtg gtggtgctgg cattgcaccg ggtgctgccc gcgctcttca
    15961 cagacgaccc gggggtgcgc gcggccgtcg cggccgggct ggtcgttgtc gctgtgggac
    16021 aggtgctttc gggctttgtc ttcgtcatcg acggggtcct catcggggcc ggggacgccc
    16081 gctggctgtc gatgtcgatg tgcctggtgc tggcggcata ccttccgatg gtgctggccg
    16141 tccgcgcctt cgcccccacc gatccacccc tcgcgatcac gtggctgtgg gtcgcgttca
    16201 ccggcttcat gctggtgcgc gcggtgttcc tcggctggcg cgtgcgctcc gatcgctgga
    16261 tggtgctcgg ggccggccgc tgatcccaca cgccggccgg ccaacggagg tgagaacggc
    16321 gatcgtccag gtctgcacgg acagggggcg ctcgcggaca accgcggcgg acgggtgctg
    16381 agccaccggt tcgcgcacgc gtgtggctca gccggccgcg cggaaggacg tgggcgagac
    16441 accgccctgc gccgcaggca acctgcactg gggcgacccc tcagtccttg cgcgccgcgc
    16501 ggatctggcg gatgcgctct ggcgggacag cgccatgttg gcccaactgt ccggcgcagg
    16561 aggtcggtga gcgcatctcc gcccgcacca ggcccagtcc agggggtcga agccgtgggc
    16621 cggcccaggt tttcggatcg ctgacatcgt cgtggacgac gagcagtatg ccgcccggcg
    16681 cccggcaatt cccccgcgcc ggaccctggg cggcagcacg tcaggcgacg ccggtggccg
    16741 cttcgcgacg gggatccccg gcggcgaaga ggacaccggt cgcgctgcgc gccgccccgc
    16801 ccacaccgcc gaagaacatc gcgtgcggct catgctcgtt cgccggcagt ccacacgcgg
    16861 cgatggccgc ccggatgtcg tcgtccctct cgaactccac ccgcgtcggc tcgccgcgga
    16921 ccacccgcac gtgggcccgc ggggcagcga tggcctcgcc gagcggcata ccgatcaagc
    16981 agtggcgggc caggacctgc atgagagccg tcgtgatccg gtcggcaccc ggactgccga
    17041 ttgcgaggac gtcgccgctg tcgtggtggg cgaccgacgg cgccatattg gacgcaagcc
    17101 gggtgccggg ggcgaggaca tgcagcccga gccggttgag ttcgggctcg ccgagacagt
    17161 tgttgaggag cagtcccgtg ccaggaaccg ttgcgccaga ggagtatccg ctcgacgccg
    17221 tgatggcgca gccgagcccg tcgctgtcga cggcggacac gtgcgccgtc gattgcgacg
    17281 tcgtcaggcc ggccaccccc gcgcgcctga cctcctcgag catgttgtag ccgtcctcct
    17341 cgaggtcggc ggagaagtcg tggacctcgc tgcgataggt caggacgtgc ctttgcacct
    17401 cgatcgtgtc cacccacgag aggctgccgc ggcgcgccat ttcgctcagc atgacggcga
    17461 gcatggggcc gccgacgctc gggggcgggt tggtcgcgat gtcccaggcg cccaggcgct
    17521 gacgcagcgg ttcgcgcacc accggccggt atgccgcgag gtcggctgcc gtgatcagcc
    17581 caccctctgc ggtgcacgcc gccgcgatcc tgcggccgat ctcgccggta tagacgtccg
    17641 cggctccatg ctcggcgatc cgggcccagc tgtcggccat gtcggtgtcg atgagcaggt
    17701 cgccgggctc gagcgcactc ccgtcctccc gggtgagcgc ctggtgcgta tcggcgtccc
    17761 agccgaagag attgtcgacc gtcagagcga gatagctggc tgccgccgag ccgagtcgaa
    17821 aaccttctcg cgcagccttt tccgcgggtg cgatggcctg agcccaggcg ccggacccgt
    17881 gccggtcatg ggcgagttgc agggccgaca aggcgccagg gatcgcgacc gaaccatatc
    17941 caccgaagac ggtgatgccg cccccgtagt tcaggtggaa ctcacgcagc ccctggccga
    18001 accgggctgg gtcgaggccc cggccgggca tctccacgtt gccgtcgatg gtctccggct
    18061 cgccgccggc gggccagatc gtcacgaacg ccccgcccat cgggctgacg acgcccggct
    18121 cggtggtcat ggcaacgcac atagcggcga tggccgcgtc caccgcattg ccgccggccc
    18181 gcaccgcggt caggcccgca tcgacagcag cgggtccggt ggcggcaacg gcgacatggt
    18241 cggtcatagg gcgattctgc ccgaccggcg cggatcgaag ctgtcgcccg tcacgagctg
    18301 ggcctcccgc atcccgggcc gtgaccgcgg cataccctga gatgcatgct gctcggggag
    18361 gaagtgctct tgctcgtcac cgacgacacg acgggtcggg cgccggcgct gtcgcagcat
    18421 ctcgatgtcg tcattggcgg gtcgctattc gccgaactca ctctgcgcga acggatcaca
    18481 ctcacgccgc acgagctcgg gtggcgcgca cgtgaccgag tccaggtgct gtaggagcgg
    18541 acgacccagg accgcatcct cgacgaggcg ctcatcgaca tcagcgcacg agcgggcaag
    18601 aagccaaggg acatattgac ccactggggc atgatcggac gcaccaggcg gtggcgcaag
    18661 gagccgtatg cccggctggt cgccgtggaa ttctcggcga aggccacgac aaggcgctcg
    18721 gcatcttcag acgcacgacg catgtcgaag tcaatcccgg ccccgagcgc gacgtccggg
    18781 ctcggctcaa cgcggccctc ttgaccgacg aggtgcccga tccgcgcacc gccaccttga
    18841 tcgccttgct ggtggcgggc gacctgctct ccaaggtgct ctcacccgag accgacacga
    18901 aggccgccaa gcggcgcgcg cacgagatcg ccgaatctga tgggccgcaa aggaagttat
    18961 gcgcacgatc cagcagatgc ggggcgccgc ggcagccggc ggcgcgggcg gcggctgacg
    19021 agctcccccc tcgagatgtc gattcgaccc cgtcgagacg tgaatgggtg cgacgcggac
    19081 ggtcaagggg ccggcgcgaa ggggaactcg gccagcacgt catacagcgg acggccctgc
    19141 cgaccctggc ccaggcgcga ctggaccagg caggcggact cggcgaccca gccgggcgac
    19201 tcatacccct gcaggatgcg gatccaacgc gtcgcctccg tcggacggcg caggcgggcg
    19261 acggtgacat gcgggtggaa ccggccgccg tcgacgacca cacccgcgtg accggcggcg
    19321 ttgcgcgcgg cccgcgccac tttgccgagc cacggctcgg gctcggcggc ggcgaacagg
    19381 acgcgggcgg catacggatt ggggaaggcg ttgccgccgc tcagccgcat cgctgccgcc
    19441 gggagggccg cgcagcgctc cgtcagcgcc tcggtcaacc ggtccagccc ggcctccccg
    19501 acgtcgggga ggaaggcgag cgtcaggtgc aggtgctccg gatcggtcca ccgcaactcg
    19561 gcaccggcgt ctcggcgcgg ccgcaggaag tcggtcaggt tctcgaccac ctcaacaggc
    19621 gggatgatgg ccacgaagag acgcatgtcc ccagtgcacc atcccgccac gaggcgtggt
    19681 ggccagggcc ggcacttttc cgcccagtgc cggttcctat cggttcgcag cggaaatgtt
    19741 gcgatgcgaa ccgcaatgaa gtggcactgg gcggaaaagt tcagctgcgg acgcgactac
    19801 cgcacgaccc cgttcgcgtt cacgatgccg aaaccgttga agccgttgcg gtcggtggta
    19861 ccgcggcaga tggcggtgta ggtctcgtca cggtcctcgt tgacgtactc ctggacgccg
    19921 ccggccgggc aggcgtggtc ggtggcggtc tgcgccatca gccagcggac cgcgccgggc
    19981 ttcatcgtca gcccgtccgc cgacttgtgg ccgtgcgcgc tgacggccag ggcggcgaca
    20041 ccggtggcgt gcggggccgc catcgacgtg ccctgcaggt actggtagaa accacaccgg
    20101 tcagcgcccg gggccggatt ggccgtgcag gccttcttga cgccgagttc cttgccgagc
    20161 ggggtgatat tccccttcgg gtccaccgcg ccctcggcct gcagcacccg cctcggggcc
    20221 gaggagagga tcatgttctc gttggtgcgg taggacgcgg tgccgaagcc gtcgcggtac
    20281 cagccgcccg gagccgagaa ctcgatctcg gtcgagtaga cctcggtcgt gtagttggag
    20341 aagtcggact tcgtgcccga tgggccgagc gaggagatgc ccagcacgtt cgggccctcg
    20401 gtcggcaagg tgacgcagcg gctgttgaag atggtgcgcc ggtgcggctc ggcgccgtag
    20461 tcggggctcg agacgtcctg gcgtggtttg gccaggtcgt cgtgctggtt gcccagggcc
    20521 gccaccagcg tgacgttctt gctggtcgcg tacttcagcg cccggttcat cgtcgcgatg
    20581 accgtctcct gctcccgcgc ctgcgcaggc gtgtcctccg gagcaccgcc ggtgcagttg
    20641 tagagccacg ggtcgacgta gaacgacata ttgacgacgt cgagtccggt gttcgcggca
    20701 tacgtcaacg cgttggatac cgggccgagg aagaagtagc cgctgtcctg cccgccgcgg
    20761 atgttgacga tcgtcgcctt gggcgcgacg ccgctcaggc ccatgccgtt catcgccgcg
    20821 gcgatggtgc cggcgacatg cgtgccgtgg ccgttgtcat cgacgttgtt ggggtccttg
    20881 cacgaggtgt actcgcactc tccgtcgaca tcggtcatgt ctgtggtgaa gttgcgagag
    20941 aggcgccggt cgaaattggc ggccaggtcg gggtggcggc cgtcgacgcc ggtgtcgagg
    21001 atcccgacac gaacccgctt gtcccccagc gtcttctggt gcgcagcgcc tgccttgatc
    21061 atgtccatgc cccatagcca gcggtccagc ggatcgccgc tgtacttcgc gcctgcacgg
    21121 tcggaatcgc tggtgccccg ttcggcggcg ggggttacgc ggttctcctt ctcgacgtcc
    21181 gacggccggg gtgcgacacc gggggagcgg ccgatggccc ggtcgcgggc tacgccctgg
    21241 accccgtcca tccggctcgt gtcgtcgacg aacgtgctgc tggtcgtgtc gaggctgatc
    21301 agcccgatat cggtgttgac actggtgatc cgcgcgccgc cggctgcgag gcggtcggcg
    21361 atggcctggg cgtcggcgcc ctcgcgcgcg agcacgacat acgatccggc ggcagcgcgc
    21421 tcggcggtcg gggcggcgga ggcggttccg gcggtggcgg cgacggcgag gccggaggcc
    21481 gcgatggcgg ccgcggcacc ggcggcgtag aaacgggtgc gttgcatgtg aagactccct
    21541 cgtgggttgt gccgtggatc acacatccgc ggccaagccc accgtagggg gaaccgccgc
    21601 ccacctgcga tctaacgaga tggatgaatc cgcaccgaac ccg
//
LOCUS       DAOPJG010000029        21427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2612_length_21427_cov_7.247660, whole genome shotgun sequence.
ACCESSION   DAOPJG010000029 DAOPJG010000000
VERSION     DAOPJG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 21427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21427
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2612_length_21427_cov_7.247660"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1386)
                     /gene="argA"
                     /locus_tag="PKU75_04530"
     CDS             complement(4..1386)
                     /gene="argA"
                     /locus_tag="PKU75_04530"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HPF80175.1"
                     /translation="MTDHPVPPRAVAPQDAASLDAETFVAALRGAAPYVHAHHGRTVV
                     IVIPGEVCARDDIDRLLGDIALLHSLGVRLVLVHGARPQIDRELAIRGQAPAYSGDLR
                     ITDEAAMEAVKAAVGVLRMDLEARLSASRSVTVKEESQPRVVGGSWVTAKPVGVRGGV
                     DLQLTGEVRRVDIAQIRRAVEQDQIVLLSPIGYSPTGETFNLRNADIAESVAIGLGAD
                     KLIFVMESEPGRWRAALGSGDAGQLSLASAEPLVSQSDRELSAEDRNCIRAGIAAVTG
                     GVGRVHLIGTQGHSPLLRELYTRDGSGLMISDDDDYESIRPARVDDARAIAELIAPLE
                     AAGILRPRSREQLELDIDTFSVITRDGMVIACNAFIDYPDDHAAEFACVVVHPAYRRR
                     DLAAHLLRRARTTARARGYRRLFALTTATPHWFLEHGFRPGTPADLPAEKRRTYDPTR
                     ASLVLLLDRP"
     gene            1458..2246
                     /locus_tag="PKU75_04535"
     CDS             1458..2246
                     /locus_tag="PKU75_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80176.1"
                     /translation="MSDVLAFAGVSVVRGGKALVKDIDWEVEEGQRWVVLGPNGAGKT
                     TLIQLASGRMHPSGGVAGILGEVLGAVDVFELRPRIGLASAALAERIPGRERVLDVVV
                     TASYGVVGRWHETYERMDEGRALELLAALGADHLAERTYGTLSEGERKRVQIARALMT
                     DPELLLLDEPGAGLDLGGREDLVARLGEIAGDVEAPALILITHHVEEIPPNFTDVLLL
                     KDGEIVAQGPLEITLTEDNLSKTFGMPLILDQFGNRYTARAGKR"
     gene            2251..2715
                     /locus_tag="PKU75_04540"
     CDS             2251..2715
                     /locus_tag="PKU75_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPF80177.1"
                     /translation="MNWLQDNAWLGWLGLAIALAAVEVATVDFVFVMLAGGALAGSLA
                     AALGAPFGAQVIVAAVVGALLVFLVRPLVRSRFLEGVSDHGIGASSLVGRSAQVVQAV
                     TEYDGRVKLGGELWSARLADGARVCEAGEEVRVVAIHGATAIVSAEKAITDR"
     gene            2833..4038
                     /locus_tag="PKU75_04545"
     CDS             2833..4038
                     /locus_tag="PKU75_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPF80178.1"
                     /translation="MEPGIIVLLLFLLLVVIVVVRTVRIVPQQTSLIIERLGRYSRTL
                     EGGIHFLVPFVDKVRANIDLREQVVSFPPQPVITSDNLVVNIDTVIYYSVIDAKSAVY
                     EIANFIQGIEQLTVTTLRNVIGSLDLEQTLTSRDQINGQLRGVLDEATGKWGIRVNRV
                     ELKAIDPPMSVQESMEKQMKAERDRRAAILNAEGVKASQILTAEGEKQSSILRAEGSA
                     QARILEAQGQARAIQQVFDAIHRGKPTQKLLAYQYLQTLPQLARGDANKMWIIPSELT
                     DALRGIGNTLGGVNTAPGPEEDEEWVDPGEELSNPFAGTELEDSTKALAEARGQAGQV
                     ENEANEHAAPAPRVQPAPDTQPQLPPPATQPMQFPYGEPQLPQVAEPQVGEPGQSVAP
                     QPPTAPPKG"
     gene            4112..4885
                     /locus_tag="PKU75_04550"
     CDS             4112..4885
                     /locus_tag="PKU75_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPF80179.1"
                     /translation="MSFWESVLVVLAGVGAGTINTIVGSGSLITFPILLLLGVPPVSA
                     NVSNNLGMLPGGIAGAYGYRRELRGRWGPLLKLLPWSVVGAAVGAALLLVLPAEAFRK
                     IVPILIGFGLLMVIFGPRLQRWSAAHHQETGAMPRWQAPALVVGLILAAMYGGYFGAA
                     QGVILMGLLSTLAHGTLQELNGWKNVLASTANFVAAVVFVSAAPEQVDWGVAALIGVG
                     SFIGGFIGAGVGRRLPPAILRAIIIAIGVVGIVKIIFFP"
     gene            4908..5720
                     /locus_tag="PKU75_04555"
     CDS             4908..5720
                     /locus_tag="PKU75_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPF80180.1"
                     /translation="MPIEVTSLDDPALRDYVGLTDVALRTRAEPERGLFIAESEKVIR
                     RALAAGYRPRSYLMGERWLTDLGDLVAEAEAGGVPVYWGPAQTLEQLTGFHLHRGALA
                     AMQRRPLPGLSEVLATARRVFILEDIVDHTNVGAIFRSAAALGVDAVLVTPRCADPLY
                     RRAIRVSMGTVFQVPWTRIDPWPGGLAVLRDLGFVTAAMALGDESLSLDELAAHAPQR
                     LALVLGTEGDGLHPRTLTHVDQVVRIPMAGGVDSLNVAAAAAVAAWALRTQP"
     gene            6275..6520
                     /locus_tag="PKU75_04560"
     CDS             6275..6520
                     /locus_tag="PKU75_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80181.1"
                     /translation="MLIRTGLGLITAIGAIGAALFLGSVSSTVMSAVLAFGAAALLYL
                     VVEELLVEAYEERESVTLGAMFFLGFILIYALSAAGG"
     gene            complement(6531..6920)
                     /locus_tag="PKU75_04565"
     CDS             complement(6531..6920)
                     /locus_tag="PKU75_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HPF80182.1"
                     /translation="MIPYQYVTLRVVPRVEREEFVNVGVVLFADEVGFLDAAFEVDED
                     RLGALFPALDLGAVEASLAVVCDVCRGVTGRGRPELPTAGKRFGWLAAPRSTVVQPGP
                     RHGGLAADPAVALEKLLDRLVRRAPIR"
     gene            complement(6917..7696)
                     /locus_tag="PKU75_04570"
     CDS             complement(6917..7696)
                     /locus_tag="PKU75_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HPF80183.1"
                     /translation="MTAQLRLDHVRATRYVTPLREGGSLPGIVEADDLGTYVVKFRGA
                     GQGLKVLVAEVIVGELARALDIPTPRLVTVDVAKDIAKYEADEEVQDLLNASLGDNLG
                     VDFLPGAFGYDGSRPAAPELAADIIWLDAFTANVDRTWNNPNLLVWHGQTFTIDHGAA
                     LYFHHSWPRRAPDAGRFASQPFDASGHVLADIAAPVPEAHARIAPRVPDLIDAVIGAV
                     PEAWLEPADGVPDPAGVRAAYAAHLRARLAGAAAWLPGGAR"
     gene            7733..8152
                     /locus_tag="PKU75_04575"
     CDS             7733..8152
                     /locus_tag="PKU75_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF80184.1"
                     /translation="MAKLNPYLTFDGTARAAIEFYAAVFGGHVKVTTFDEFGDAGNHG
                     VMHAQLDTLEGFTLMASDLPPGGGADHVVGTNVAVSLSGMEADLLREYWHRLSSGATV
                     TLPLEKQMWGDEFGMLIDRFGVHWMVNIAAAPAAAAP"
     gene            complement(8291..8758)
                     /locus_tag="PKU75_04580"
     CDS             complement(8291..8758)
                     /locus_tag="PKU75_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HPF80185.1"
                     /translation="MTDTTRVTVERVIPASTAAIFDILSNPLRHADLDGSGFIRSDAG
                     AQRIQAVGDVFTMNMEGEHMGGEYQTDNHVTGYADNALLAWQTAPAGTEPPGWEWVWE
                     LEPQGPDSTLVRHTYDWSKVTDKELLQKVKFPLVTEAQLEDTLVKLDEAATGS"
     gene            complement(8870..10036)
                     /locus_tag="PKU75_04585"
     CDS             complement(8870..10036)
                     /locus_tag="PKU75_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPF80186.1"
                     /translation="MPEVTWEDLAAHALRRQFPEPPSRTPGVADVVDLLQRVGPLQTQ
                     NARAAFIGIAARLPGVTHEVITAAYEEHRILRGSNLRGTVHTSTAADHPLLEATTRIG
                     QRALYHRHLRPVATSLDEIFAGIEDYARDEWRTPAQLREHLGAWMSEHDPQGQPQLET
                     TAGRYFGFGHGGLVRRPLSGPWSGQGAPGYRTASALLAPADIGERTRLLADSDAAVEA
                     MIRRHLTAYGPVSRNDIAWWSGVGLTPIDAGLQRLAAELHSMTGPDGRTYVTLTTPAA
                     PSDADIPGIALLPEFDALLCGFDPPARGRFVDPRHYDILWRQENGLLLAPLLVRGRLT
                     GYWTLAGTGGRRELTVSYFRGTPRPTKSLLTAPVAGLERALGVRVGTVAIARHS"
     gene            complement(10029..11852)
                     /locus_tag="PKU75_04590"
     CDS             complement(10029..11852)
                     /locus_tag="PKU75_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80187.1"
                     /translation="MSRSEPTATTAEQELEPMPAGWPSMLRIVRHGYRAEPKLLSASL
                     AMTVLEALPDVLVAVWLALITRGIVNHDRDDLLLGALGLAASATATWALQVTLGRTKR
                     RLGDRLNIYFQGYVATLQAKVATLEHHERPGYLDRIAVLRTGVFALDHLFASILTMVG
                     WFVRLAFVSVLLATIHPALLLLLLAALPLLVVATWRPKVEKAAEEAVAPHGRLGRHLF
                     LLGTSAGPGKEIRVTGNQDDLRARRRRAWESWFAPQRAASLVTAAWLALTWAIFGVAF
                     VVAVAWIAHRPGVTAASIVLVLAAGSRLTAYVGSAVGELGFLRGIWLDSAKRLAWLED
                     FAQSTAGTGTQAPPDRLTDGIRFDGVGFAYPGTTRRVLDGVDLHLPAGSVVAVVGENG
                     AGKSTLVKLLARMYEPTAGRITVDGTDLSALDLRGWRERISGAFQDFARLEFRAHTTV
                     GLGDLPRLDDRPAVAAAVERAGATPVVDQLAGGLETQLGPSWDDGVDVSFGQWQKLAL
                     ARGYMRHEPLLLMLDEPTAALDAETEHAIFEQFTAGARSENDTGRVTILVSHRFSTVR
                     MADLIVVLDGSRVAEVGSHEALMARGGLYAELYTIQAGAYA"
     gene            complement(11839..13662)
                     /locus_tag="PKU75_04595"
     CDS             complement(11839..13662)
                     /locus_tag="PKU75_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80188.1"
                     /translation="MARTQLTQRPSWRFFVTMTRAVPGLSAVWWALLALRSLLPAALA
                     VCTGWLVGAVAAGSDLTTPLTAFGILFVAAQVAPPLHQVIGTLLGHRLSNELNERLLT
                     ATLEPDGIGHLERGDLSNDLTTARDFDLGMTGPPMFINMDFVAGSVVLLLAGVASALV
                     LFGYSWWAPLLLLAAWGSTHWLLRESGVWRDRNTDEVKSARRHTDYAYTLATEPAAAK
                     EVRLFGFDDWLLERFRTNRLRLHELQYAATRLRERSLLGALVIVLCANGFLLFMIARD
                     AYAGALTLQQTVTFAGAAFGASLIAFGGLSWAMDVASAPVMALEALEPEMHAAGSLVT
                     PPPRPGDRSPPPRRGAPEIRFEKVTFAYPGTDRPVLRDFDLTIPAGSSFAIVGINGAG
                     KTTIAKLLCRLYDPQAGAIRIDGVDLRELPIAPWRSELAAVFQDFVRYELPLRDNVMP
                     LGGEDAAASAALAAAGADRLAGLDTPMAKGYPGGIDVSGGQWQRIALARAIAKVEAGA
                     DVVLLDEPTAMLDVRGEAEIFSRVLDATAHATTILISHRFSTVRQVDHICVVEEGRAV
                     EVGSHDELVALGGRYRTMYDVQASRFVELDDEGEEVVYESL"
     gene            complement(13733..15256)
                     /locus_tag="PKU75_04600"
     CDS             complement(13733..15256)
                     /locus_tag="PKU75_04600"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPF80189.1"
                     /translation="MQLSHLVATSNALVATSARNAKIALVAEALQAAGPEEVGLAAAY
                     LSNVLPQRRLGVGWRGLSDLPAPAADASLTLVDVDAAFDALATAGGSGSVASRRAVVS
                     HLLARATGPEQEYLRGLMLGGVRQGAQDGVMLAAIAKAADVPLAAVQRAVMLSGFAAP
                     VAQAALSGGVDALAAMTLEVLRPLRPMLAGSSPDIAGAFRDGADVFIERKIDGIRLQA
                     HKAGADVRLFTRSLDEITDRLPEVVELVAALPCDRVVLDGETIAMRPDGRPAPFQVTG
                     ARTASSADPEALRRTAPLATSFFDLLHLDGEDLLDHRLAERISRLDALLPEQARMVRI
                     TTDSPADAESFFAAQVAAGHEGVMVKDPASPYAAGRRGAGWVKVKPRHTLDLVVLAVE
                     WGSGRRRGTLSNIHLGARDPDTGGFVMLGKTFKGMTDAMLAWQTERFLELETHRDSYT
                     VHVRPEQVVEIAFDGIQKSSRYPGGMALRFARVLRYRDDKSADQADTIETVRSLRGW"
     gene            15677..>16055
                     /locus_tag="PKU75_04605"
     CDS             15677..>16055
                     /locus_tag="PKU75_04605"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80190.1"
                     /translation="MAFISASINVFSAATRGLLVLLVTGPLGVPPEMFGLFIAVPAAG
                     AIVGSLAAERVVPAVGGGPITWLAALVPAASFAALGLTSNVPVILIAEFLAAVASSLN
                     QIVVSTLRQAAVPDALLGRVTAAY"
     assembly_gap    16056..16155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16265..16348)
                     /locus_tag="PKU75_04610"
     tRNA            complement(16265..16348)
                     /locus_tag="PKU75_04610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16312..16314),aa:Leu,seq:gag)
     gene            16467..17387
                     /locus_tag="PKU75_04615"
     CDS             16467..17387
                     /locus_tag="PKU75_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPF80191.1"
                     /translation="MQVLEPREVPLGGLRAMLVRRTLPHRDRSFIGAWCFADHYGPTR
                     VGADGPGMDVAPHPHTGLQTVSWLFDGEIEHRDSGSFHALVRPGEINLMTAGRGVAHS
                     EVSTPTTEVLHGVQLWVALPEEARHVERTFEHHAAPVMELPAGAGAARVFLGELRDIA
                     ASPVHTFTPLLGAQLDLTPGAEVTITLDPAYEHGVLLDTGDLRVDGAELHTGSLACVD
                     AGPTSLRLVTDQGARLILLGGKPFEEEIVMWWNFVGRSHEEVATLRGEWEAGSERFGT
                     VQGYAGARPRIPAPPLPGGRLRPRGRLRRA"
     gene            17401..20067
                     /locus_tag="PKU75_04620"
     CDS             17401..20067
                     /locus_tag="PKU75_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HPF80192.1"
                     /translation="MPAPLRDIATAANADEVLERFEEWASGRGIRLYPAQEEAVLTLL
                     TGANVILATPTGSGKSLVAVAAHATAMAAGERSWYTAPIKALVSEKFFDLIQIFGADN
                     VGMLTGDAAVNADAPIICATAEVLANQALRERDRADIGLVVMDEFHFYAEPDRGWAWQ
                     VPLLLLPRTQFLLMSATLGDTTFFEEDLTRRNGRDTVAITSAERPVPLHFDWVLTPIH
                     ETIEDLLHEGRAPIYVVHFTQQAAVERAQALMSVNVSTSAEKEEIKRLVGGFRFGKGF
                     GMTLKRLVLHGIGVHHAGMLPKYRRLVETLAQAGLLKVVCGTDTLGVGINVPIRSVLL
                     TSLTKYDGRRQRVLRAREFHQIAGRAGRAGYDTEGFVVVQAPEHLIENHRALLKAGDD
                     PKKRRKVQRKGPPEGQLSYTEGTYDRLVAAPPEPLRSRMRVTHGLVLNLLDEVEGQGE
                     AMRIVLEDNHEEAVSQSRLVVRAAQIIASLEKADVVAPVPGAEGFEVTVPVPDAAEVL
                     DEPDEQRVAELEIEAGAAADGVDDPDVEEVTSADPLDVRGTLATAYEIVDDLQDGFAL
                     NQPLSAFALAVLDVLDETEPDYALDVVSVIEATLEDPRQVLMAQQFAARGEAVAQMKA
                     DGLEYEERLELLEDVSWPKPLQELLTDAFAIYRQSHPWVYEVDLSPKSVVREMYEHAM
                     TFGEYVGRYQLGRAEGILLRYLSDAYRALRQTVPLGYRDDELDDVIEWLGELIRQTDS
                     SLVDEWESLMNPADAGPEVRPETSRALSANPRALRVMVRKAMFRRVELLALQRYAALA
                     ALAGPLTEAQWREAGEDYFAEYDHLGTGADARGPAYLRIEEEPEEWVVTQILEDPDGD
                     RDWRLTATLDLAETDAAGEPVWTRMDLGPATR"
     gene            20592..>21427
                     /locus_tag="PKU75_04625"
     CDS             20592..>21427
                     /locus_tag="PKU75_04625"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPF80193.1"
                     /translation="MDLPVMPPVQPMLAKSAGGIPDPAKFDGGLSFEPKWDGFRCLVF
                     KDGDEVELASRNTKSLARYFPEVVAALRDCLPEKVVLDGEIFVAVGDRLEFEVLQERI
                     HPAASRIKLLSEQTPAQYVAFDLLALAEEDLMAKPFAERRAELVDVLTAPSDTVHITR
                     TTTDPAIAQEWFETFEGAGLDGVVAKPLGAPYVPNARSMIKIKQARTADVVLAGYRLH
                     KNSTAAEPLLGSLLLGLYDDEGHLAHIGVSASFTAAKRKSLLAELKPLEEGVDDHPWG
                     EWA"
BASE COUNT         3253 a         7308 c         7564 g         3202 t
ORIGIN      
        1 gggtcagggg cggtcgagga ggaggacgag ggaggcccgg gtcgggtcgt aggtgcggcg
       61 cttctcggcg ggcaggtcgg cgggggtgcc gggacggaaa ccgtgctcga ggaaccagtg
      121 cggcgtggca gtggtcaagg cgaacaggcg ccggtacccc cgcgcccggg ccgtcgtgcg
      181 cgctcggcgc agcaggtgcg cggcgaggtc gcgccggcgg tatgccgggt gcaccaccac
      241 gcacgcgaac tcggctgcgt ggtcgtcggg gtagtcgatg aaggcgttgc aggcgatgac
      301 catcccgtcc cgggtgatga ccgagaaggt gtcgatgtcc agctcgagtt gctcgcggct
      361 gcgtgggcgc aggatccccg ccgcctccag cggtgcgatg agttccgcga tggcgcgggc
      421 gtcgtcgacc cgcgccggcc ggatcgactc gtagtcgtcg tcgtcggaga tcatcagccc
      481 cgacccgtcc cgcgtataga gctcacgcaa caacggcgaa tgcccttgtg tcccgatcag
      541 gtggacgcgc ccgacgccgc cggtcactgc ggcgatgccc gcccgtatgc agttgcggtc
      601 ctccgcgctc agctcgcgat cgctctggct caccaggggt tcggccgacg ccagggacag
      661 ctgccccgcg tcgccgctgc cgagggcggc gcgccagcgc ccgggttcgg actccatgac
      721 gaagatgagt ttgtcggcgc cgagcccgat ggcgaccgac tcggcgatgt cggcattgcg
      781 caggttgaac gtctcgcccg tgggggagta gccgatcggg gagagcagca cgatctggtc
      841 ctgctcgacg gcacgccgga tctgggcgat gtcgacgcgc cgcacctcgc cggtgagctg
      901 aagatcgacg ccgccgcgca caccgacagg cttggccgtc acccacgacc cgccgacgac
      961 ccgtggttgt gactcctcct tgaccgtgac gctccggctc gccgagagcc gcgcctccag
     1021 atccatccgc agcaccccaa ccgcggcctt gacagcctcc atcgcggcct cgtcggtgat
     1081 ccgcaggtcc ccgctgtatg ccggcgcctg cccccggatc gccaactccc ggtcgatctg
     1141 gggccgggcg ccgtgaacga gaaccagacg cacgccgagg gagtgcagca gggcgatatc
     1201 accgaggagc cggtcgatgt cgtcgcgggc acacacctcg ccggggatga cgatgaccac
     1261 ggtgcgccca tggtgggcgt gcacataggg cgcggcgcca cgcaacgccg cgacgaacgt
     1321 ctcagcgtcc aaggacgcgg cgtcctgcgg agcgaccgcg cgcggcggga cggggtgatc
     1381 ggtcacagcc cgaagcgtag ccacgccgca ctcgcacgcg ttcgggagga caccggacgg
     1441 caggtagggt cgagagcatg agtgacgtcc tcgccttcgc cggcgtgtcg gtcgtgcgcg
     1501 ggggcaaagc cctggtcaag gacatcgact gggaggtcga ggaggggcaa cgctgggtgg
     1561 tcttgggccc caacggtgcc ggcaagacga cgctgatcca gctcgcctcc gggcgcatgc
     1621 accccagcgg cggcgtcgcg ggcatcctcg gtgaggtact gggcgccgtc gacgtcttcg
     1681 agttgcgtcc ccgcatcggc ctcgcctcgg ccgctctcgc cgaacgcatc cccggccgcg
     1741 agcgcgtcct ggatgtggtg gtgaccgcgt cctacggcgt cgtcggccgc tggcacgaaa
     1801 cctacgaacg gatggacgag gggcgtgccc tcgagttgct cgccgcgctc ggcgccgacc
     1861 acctggccga gcggacatac ggcaccctgt ccgaaggcga gcgcaagcgc gtccagatcg
     1921 cgcgcgccct gatgaccgac cccgaactgc tgctcctcga cgagcccggc gccgggctcg
     1981 acctcggtgg gcgcgaggat ctcgtggccc gcctcggcga gatcgccggc gatgtcgagg
     2041 cccccgccct gatcctcatc acccaccacg tcgaggagat cccgccgaac ttcacggacg
     2101 tgctgctgct caaggacgga gaaatcgtcg cgcagggtcc cctcgagatc acgctcacgg
     2161 aggacaatct cagcaagacc ttcggtatgc cgttgatcct cgatcagttc ggcaaccgct
     2221 acaccgctcg cgcaggaaag aggtgacggc gtgaactggt tgcaggacaa cgcctggttg
     2281 gggtggctgg gtctggcgat cgcgctcgcc gcggtggaag tcgcgacggt cgatttcgtc
     2341 ttcgtcatgc tcgccggcgg cgccctcgcc ggttcgctgg ctgccgccct cggtgcaccg
     2401 ttcggtgctc aggtcatcgt cgccgcggtc gtaggtgccc tcctggtctt cctcgtccgt
     2461 ccgctggtgc gctcccggtt cctcgagggc gtcagcgatc acgggatcgg ggcgtcctcg
     2521 ctcgtgggtc gcagtgccca ggtggtccag gcggtcacgg agtatgacgg ccgggtcaag
     2581 ctcggcggtg aactctggtc ggcgcgtctc gccgacggcg cgcgagtgtg cgaggcgggc
     2641 gaagaggtgc gcgtggtggc gatccacggc gccaccgcga tcgtcagtgc cgagaaggcg
     2701 atcaccgatc gctaggcgtc cgcgcgactc cggtgacacc ccgagggaac cggagcggcg
     2761 gcataccact caaatcgtgt gaagggggcg gccaccgggc cgttcacatc agcagaacag
     2821 aaagtagtca ccatggaacc gggcatcatc gtcctcctcc tgttcctcct cctcgtcgtc
     2881 atcgtcgtcg tgcgcacggt gcggatcgtg ccgcagcaga cgtcgctgat catcgagcgg
     2941 ctgggtcgct attcacgcac cctcgaaggc ggcatccact tcctggtgcc cttcgtggac
     3001 aaggtgcgcg ccaatatcga cctgcgcgag caagtcgtct cgttcccgcc gcagccggtg
     3061 atcacctccg acaacctggt cgtgaacatc gacaccgtca tctactactc ggtgatcgac
     3121 gcgaagtcgg ccgtctacga gatcgccaac ttcatccagg gcatcgagca gttgaccgtc
     3181 accacgctcc gcaacgtcat cggatcgctc gaccttgagc agacgttgac ctcccgcgac
     3241 cagatcaacg ggcagctgcg gggcgttctc gacgaggcga ccggcaagtg gggcatccgc
     3301 gtcaaccgcg tcgagctgaa ggccatcgac ccgccgatgt cggtgcagga gtcgatggag
     3361 aagcagatga aggcggagcg ggaccggcgc gccgccatcc tcaacgccga gggtgtcaag
     3421 gccagccaga tcctcaccgc ggaaggcgag aagcagtcct cgatcctgcg ggcggagggc
     3481 tcggctcaag cccgcatcct cgaagcgcag ggccaggcgc gtgccatcca gcaggtcttc
     3541 gacgcgatcc accgtggcaa gccgacccag aagctgctcg cctaccagta ccttcagacc
     3601 ctcccgcagc tggcccgcgg cgacgcgaac aagatgtgga tcatcccctc ggagctgact
     3661 gacgccctgc gcggcatcgg caacaccctc ggcggagtca acaccgcccc ggggccggag
     3721 gaggacgagg agtgggtcga cccgggcgag gagctgagca accccttcgc gggcaccgag
     3781 ctcgaggact ccacaaaggc gctggccgag gcgcggggcc aggccgggca ggtcgagaac
     3841 gaggccaacg agcacgccgc gcccgccccg cgcgtgcagc ccgccccgga cacgcagccg
     3901 cagctgccgc ccccggcgac ccagccgatg caattcccgt atggcgagcc gcagctcccg
     3961 caggtcgcgg aaccgcaggt gggcgagccc gggcagagcg tggcgccgca gccgcccacc
     4021 gcgccaccga agggctgaag cgcaccggac cgcaccgcat acccgcggac gcgaccttcg
     4081 ccacccgcac acccttgatt agggtgtgcg ggtgtcgttc tgggagtcgg tgctcgtggt
     4141 gctcgcgggc gtgggggccg gcaccatcaa caccatcgtc ggctcgggtt ccctcatcac
     4201 gttcccgatc ctgctgctgc tcggggtgcc ccccgtctcg gcgaacgtct cgaacaacct
     4261 cggcatgctg cccggcggga tcgccggcgc ctacggatac cggcgggaac tgcgcggccg
     4321 ctggggtccg ctgctgaagc tgctgccgtg gtcggtggtc ggtgcggcgg tgggtgcggc
     4381 gctgctgctc gtgctgccgg ccgaggcctt ccgcaagatc gtgccgatcc tgatcgggtt
     4441 cggcctgttg atggtgatct tcgggccgcg gttgcagcgg tggagcgcag cgcaccatca
     4501 ggagacgggt gcgatgccgc gttggcaggc gcccgctttg gtcgtcggac tcatcctcgc
     4561 cgcaatgtat gggggctact tcggggcggc gcagggtgtc atcctcatgg ggctgctctc
     4621 cacgctcgcg cacgggaccc tgcaggaact caacggctgg aagaacgtgc tggcgtccac
     4681 ggcgaacttc gtggccgcgg tcgtcttcgt cagcgccgcg cccgagcagg tcgactgggg
     4741 ggtcgccgcc ctgatcggcg tcggcagctt catcggcgga ttcatcggcg ccggggtggg
     4801 gcgccggctg ccgccggcca tcctgcgcgc catcatcatc gcgatcggtg tggtcggcat
     4861 cgtcaagatc atcttcttcc cctgaccacc gcctgcgaag gacccgcgtg cccatcgaag
     4921 tgacctcgct cgacgatccc gcgctgcgcg actacgtcgg gctgaccgat gtcgccctcc
     4981 gcacgcgcgc cgagccggag cgcggtctgt tcatcgcgga gtcggagaag gtgatccgtc
     5041 gggcgctcgc ggcgggctac cggccacggt cctatttgat gggggagcgg tggctcaccg
     5101 acctgggtga tctcgtcgcc gaggcggagg cgggcggagt gccggtctat tgggggccgg
     5161 cacagacgct ggagcagctg accggcttcc acctgcaccg cggggcgctg gcggccatgc
     5221 agcgccggcc gctgccgggg ctcagcgagg tcctggccac ggcccgtcgg gtgttcatcc
     5281 tcgaagatat cgtcgaccac accaatgtcg gggcgatctt ccggtcggcc gcggcgctgg
     5341 gcgtggatgc ggtgctcgtg acgcccaggt gcgcggatcc gctctaccgc agggcgattc
     5401 gcgtgtccat gggcaccgtc ttccaggtgc cctggacccg aatcgacccg tggccaggtg
     5461 gcctcgccgt cctgcgtgac ctcggcttcg tcacggccgc aatggcgctg ggggatgagt
     5521 cgctttcgct cgacgagctt gcggcgcacg cgccccagcg gctggcgctc gtgctcggca
     5581 ccgagggcga cgggttgcac ccgcgcacgc tgacgcacgt cgatcaggtc gtgcggatcc
     5641 ccatggccgg cggcgtcgac tccctcaacg tcgctgccgc tgcggcggtc gcggcgtggg
     5701 ccctgcgcac acagccctga gcagtgttca catgagaaca taagtctcgt tgccacggcc
     5761 gccggtctcg ttgccttccc cgtcgccgca tccacgatcg gcgcactggt ggccgccgtg
     5821 agaccacccg gcccgcgggt ggtcagcggc gtgcagcact tcgcggcggg ggttgtgaat
     5881 gccgccttgg tcggcgagat catgcccgaa ctgcgtgacg aggggcacgt gctgtggacg
     5941 atcgtcggct tcgtcgccgg ggcggtcctt gtgcgggcgc tcggggagta cggccggcat
     6001 agcgaggaag cggccgccgt ggccgccgca gcccatccga agggatcgct tcggcggcag
     6061 gcggtgccgc cggcgtggcg aagaccttgc cgatcggcgt gctcggggcg atcgcggtcg
     6121 atctgctcct cgatggcgtg ctcgtcgggc tcgggtccgc ctcggctcga ctcaaggcat
     6181 catcctgacc atcgccctga ccctggagat cctcttcctc ggcctctcgg tcgtctccga
     6241 gttgaccgag gcgggggcca gcagacagcg ggctgtgctc atccgtaccg gactggggct
     6301 gatcaccgcg atcggcgcca tcggcgctgc actcttcctc ggttcggtga gcagcacggt
     6361 gatgagcgca gtgcttgcct tcggcgcggc cgcactgctc tatctcgtgg tcgaggaact
     6421 gctggtcgaa gcctatgagg agcgagagtc ggtcaccctc ggggcgatgt tcttcctcgg
     6481 gttcatcctc atttacgccc tctccgcagc ggggggctga cggcgcggcg ctagcggatc
     6541 ggcgcccgcc ggaccaggcg gtccagcagc ttctcgagtg caacggcagg atcggcggcg
     6601 agaccgccgt gccgcggccc gggctgcacg acagtgctgc gcggagcggc gagccagccg
     6661 aatcgttttc cggcagtggg gagttcggga cgtccacggc cggtgacacc gcggcatacg
     6721 tcgcaaacca cggcgaggga cgcctcgacc gcgcccaggt cgagcgcggg gaagagcgcg
     6781 cccagccggt cttcgtcgac ctcgaacgcc gcatcgagga agcccacctc gtcggcgaag
     6841 agcaccaccc ccacgttgac gaattcctcc cgctcgacgc gggggaccac gcgcagcgtc
     6901 acgtattggt aggggatcac cgggcacctc ccggcagcca cgccgcggcg ccggcgaggc
     6961 gggcccgcag atgcgctgcg tatgccgcgc gcacgcccgc ggggtcgggg accccgtcgg
     7021 ccggctccag ccaggcctcg ggcaccgcgc cgatgacggc gtcgatcagg tccggcacgc
     7081 gcggggcgat ccgtgcgtgc gcttcgggga ccggtgcggc gatatcggcc agcacatgac
     7141 cggaggcgtc gaagggctgc gaggcgaacc ggccggcgtc cggagcccgc cggggccatg
     7201 aatggtggaa gtaaagcgcg gcgccatggt cgatggtgaa cgtctgccca tgccagacaa
     7261 gcagattcgg gttgttccac gtgcgatcga cgttggcggt gaaggcgtcg agccagatga
     7321 tgtcggcggc gagttcggga gcagcggggc gcgacccgtc atacccgaaa gcgcccggca
     7381 ggaagtcgac gccgaggttg tcgccgaggc tggcgttgag gaggtcctgg acctcctcgt
     7441 ccgcctcgta cttggcgatg tccttggcga cgtcgacggt cacgagccga ggggtcggga
     7501 tgtcgagcgc tcgggcgagc tcaccgacga tgacctcggc cacgaggacc ttcagcccct
     7561 gcccggcgcc gcggaacttc acgacatagg tgccgagatc gtccgcctcg acgatgcccg
     7621 gcaggctgcc cccctcgcgc aggggcgtga cgtagcgggt ggcacgcacg tggtcgaggc
     7681 gaagctgggc agtcatcgcg ccagagtatg ccgcgcgcat agggttgatc gcatggcgaa
     7741 actcaatccc tatctgacct tcgatggcac ggcgcgggcc gcgatcgagt tctacgccgc
     7801 cgtcttcggg ggacatgtca aggtcacgac cttcgacgag ttcggcgacg cgggcaacca
     7861 cggcgtcatg cacgcccagt tggacacgct cgagggcttc accctcatgg cgtccgatct
     7921 gccaccgggc gggggcgccg accacgtggt cggcaccaat gtcgcggtca gcctgagcgg
     7981 gatggaggcc gacctgctgc gcgagtactg gcatcgcctg tcatcgggcg cgacggtgac
     8041 tttgccgttg gagaagcaga tgtggggcga tgagttcggg atgctcatcg accgcttcgg
     8101 cgtgcactgg atggtcaaca tcgctgccgc acccgcagcc gccgcgccct gatcccgcat
     8161 tcctcactcc ggacaacctg actccgcagg accttgactc ccgacataac gcgagggccc
     8221 gagaccgcag ccggtcggtg acctgcgctg ccggcctcgg gcccgcccgc gacgaaactc
     8281 gatggagggg ttacgagcct gtcgcggcct cgtcgagctt gacgagggtg tcttcgagct
     8341 gggcctcggt gacgagcggg aacttcacct tctgaaggag ctccttgtca gtcaccttgg
     8401 accagtcgta ggtatgccgg accaacgtgc tgtcggggcc ctggggttcc agctcccaga
     8461 cccattccca cccgggcggc tcggtgccgg ccggcgcggt ttgccaggcg agcagtgcgt
     8521 tatcggcgta gccggtgacg tgattgtccg tctggtactc accgcccatg tgctcgcctt
     8581 ccatgttcat ggtgaagacg tcgccgacgg cctggatgcg ttgggcgccg gcgtcggagc
     8641 ggatgaagcc ggatccgtcg aggtcggcgt gccgcagcgg gttgctcagg atgtcgaaga
     8701 tggcggcggt ggacgcgggg ataactcgct ccaccgtgac tcgtgtggtg tctgtcatcc
     8761 ctcgaatcta cgaacgatcc gccccgaaat gggccgcagc ggcctgtgga tcggtggggg
     8821 acacccctgc gggtgacccg ggatgccggc ctgcggctgg cgcaggcggc taggagtggc
     8881 gggcgatcgc gacggtgccg acccgcaccc cgagcgcccg ctcgaggccg gcgaccggag
     8941 ccgtgagcag gctcttggtg ggtcggggcg tgccgcggaa ataactgacg gtcagctcac
     9001 gccggccacc ggtcccggcg agggtccagt aaccggtcag gcgcccgcga acgagcagcg
     9061 gcgcgaggag gaggccgttc tcctgccgcc acaggatgtc gtaatggcgc gggtcgacga
     9121 atcggccccg ggccggtggg tcgaacccgc acaaaagcgc gtcgaactcg ggcagcaggg
     9181 cgatcccggg gatgtcagcg tcgctggggg cggcgggggt cgtgagggtg acgtaggtgc
     9241 gcccgtctgg accggtcatg ctgtgcagtt ctgcagccag ccgctgcagg ccggcgtcga
     9301 tgggggtcag tccgaccccc gaccaccagg cgatgtcgtt gcggctcacc ggaccgtatg
     9361 ccgtcaggtg gcgccggatc atcgcctcga cagccgcatc ggagtcggcg agcagccggg
     9421 tgcgctcgcc gatgtcggcc ggcgccagca gcgcggacgc ggtccggtat cccggggcgc
     9481 cctgccccga ccacggaccc gacaggggcc ggcgcaccag gcccccgtga ccgaagccga
     9541 aatagcgtcc cgcggtggtc tccagctgcg gctgcccctg ggggtcgtgc tcggacatcc
     9601 acgcgccgag gtgttcgcgc agctgcgccg gggtgcgcca ctcgtcgcgg gcgtagtctt
     9661 cgatgccggc gaagatctcg tcgagactcg tcgcgacggg gcgcagatgg cggtgataga
     9721 gcgcccgttg cccgatgcgg gtcgtggcct cgagcagcgg atggtcggca gcggtggagg
     9781 tgtgcaccgt gccgcgcagg ttgctgccac gcagaatccg gtgctcctcg tatgccgcgg
     9841 tgatcacttc gtgggtcacg ccgggcaggc gggccgcgat gccgatgaag gcggcccgag
     9901 cgttctgcgt ctgtagcggt ccgacccgct gcagcaggtc gacgacgtcg gcgacacccg
     9961 gtgtccggct gggtggctcg ggaaactgcc gccgcaaggc gtgcgcggcc aggtcctccc
    10021 aggtgacctc aggcataggc gccggcctgg atggtgtaga gctcggcata caacccgccc
    10081 cgggccatca gggcctcgtg actgccgacc tcagccactc tgctgccgtc gaggacgacg
    10141 atcaggtcgg ccatcctgac cgtcgagaag cggtgtgaga caaggatggt gacacgcccc
    10201 gtgtcgttct cactgcgcgc tccggccgtg aactgttcga agatcgcgtg ctcggtctcg
    10261 gcgtcgaggg cggcggtcgg ctcgtcgagc atcagcagca gcggttcgtg acgcatatag
    10321 cccctggcga gggcgagctt ctgccactga ccgaagctga cgtcgacacc gtcgtcccag
    10381 gacggaccca actgggtctc gagtccgccg gcgagttggt ccacgaccgg cgtggcaccg
    10441 gcccgctcga cggctgccgc gacggccggc cgatcgtcga ggcgggggag gtcaccgagc
    10501 cctacggtcg tgtgagcacg gaactccagt cgggcgaagt cctggaaggc gccgctgatc
    10561 cgctcgcgcc agccgcgcag gtcgagagcg gacaggtcag tgccgtcgac cgtgatccgc
    10621 ccggcggtgg gctcgtacat gcgggccaac agcttgacca gggtcgactt gccggcaccg
    10681 ttctcgccga cgaccgcgac cacgctgccg gcgggcaggt gcaggtcgac gccgtccagg
    10741 acccggcggg tggtgccggg ataggcgaag cccaccccgt cgaaccggat gccgtcggtg
    10801 agccggtcgg gcggggcctg cgtgcccgtg cctgcggtgc tctgcgcgaa gtcctccagc
    10861 cacgccagcc gcttggccga gtcgagccag atcccgcgca ggaagccgag ttcgccgacg
    10921 gccgagccga cgtatgccgt cagccggctc cccgcggcga gcaccagcac gatggacgcg
    10981 gcggtgacgc cgggccggtg ggcgatccag gccacggcca cgacgaaggc gacgccgaag
    11041 attgcccagg tcagagcaag ccatgcggcc gtgacgaggc tggccgcgcg ctgcggcgcg
    11101 aaccacgatt cccaggcccg gcgacgccgg gcccgcagat cgtcctggtt gccggtcacc
    11161 cggatctcct tgccgggccc ggcgctggtg cccaggagga agaggtgacg gccgaggcgt
    11221 ccgtgggggg cgaccgcttc ctcggctgcc ttctcgacct ttggacgcca ggtggccacg
    11281 acgagcaggg gtagcgcggc caggagcagg agcagcagtg ctgggtggat cgtggcgagg
    11341 aggaccgaga cgaaggccag ccggacgaac cagcccacca tggtgaggat cgaggcgaag
    11401 aggtgatcaa gggcgaagac gccggtgcgc aacacggcga tccggtcgag atagccgggc
    11461 cgctcatggt gttcgagggt ggcgaccttg gcctgcaagg tcgcgacgta accctggaaa
    11521 tagatattga gccggtcacc gagacggcgt ttggtgcgcc cgagcgtgac ctgcagcgcc
    11581 caggtggcgg tggccgaggc agccaggccg agggcgccga gcaggaggtc gtcgcggtcg
    11641 tggttgacga tgccgcgcgt gatcagggcc agccagacgg cgacgaggac atccggcagg
    11701 gcctcgagca cggtcatcgc gagcgaggcg ctgagcaact tcggctccgc ccggtagccg
    11761 tgccgcacga tgcgcagcat cgacggccag ccggccggca tcggctcgag ttcctgttcc
    11821 gcggtcgtgg cggtgggctc agagcgactc atagacgacc tcttctccct cgtcgtcgag
    11881 ttcgacgaag cggctggcct gcacgtcgta catcgtccgg tagcgaccgc cgagcgcaac
    11941 cagttcgtcg tggctgccga cctcgaccgc gcggccctcc tcgacgacgc agatgtggtc
    12001 gacctgccgg acggtggaga accggtgcga gatgaggatc gtcgtggcgt gcgccgtggc
    12061 atccaggacc cgggagaaga tctccgcctc gccgcggacg tcgagcatcg cggtcggctc
    12121 gtcgaggagc acgacatccg cgcccgcctc gaccttggcg atcgcgcgcg ccagggcgat
    12181 ccgttgccat tgcccgccgg agacgtcgat tccgccggga tagcccttgg ccatgggcgt
    12241 gtccagcccg gccagcctgt cggcgcctgc cgcggcgagg gcggcgctcg cggcggcgtc
    12301 ctcgccaccg agcggcatga cattgtcgcg cagcggtaac tcgtagcgca cgaaatcctg
    12361 gaagacggcc gccagttcgc tgcgccacgg cgcgatcggt agctcccgca ggtccacccc
    12421 gtcgatgcgg atggcgcccg cctgcgggtc atagaggcgg cacaggagtt tggcgatcgt
    12481 cgtcttgccg gccccattga tccccacgat cgcgaagctg gacccggcgg ggatggtcag
    12541 gtcgaagtcg cgcagcaccg gccggtcggt gccgggatag gcgaacgtga ccttctcgaa
    12601 gcggatctcc ggggcaccgc gccggggagg tggcgaccgg tcgccgggcc gcgggggcgg
    12661 ggtgacgagg gacccagcgg cgtgcatctc gggttcgagg gcctccaatg ccatgaccgg
    12721 cgcggacgcc acgtccatcg cccacgacaa cccgccgaag gcgatcaggc tcgcgccgaa
    12781 cgcagccccg gcgaaggtga ccgtctgctg gagagtcaga gcgccggcat acgcatctcg
    12841 ggcgatcatg aaaagcagga aaccgttggc gcacaacaca atcaccagcg ctccgaggag
    12901 cgagcgttcg cggaggcggg tggcggcata ctgcaactcg tgcaaccgca accggttggt
    12961 gcggaagcgc tccagcaacc agtcgtcgaa gccgaacaac cgcacctcct tcgccgccgc
    13021 aggctcggtg gccaaggtgt aggcatagtc ggtatgccgg cgggccgact tgacctcgtc
    13081 ggtgttgcga tcgcgccaga cgccgctctc gcgcagcagc cagtgcgtcg atccccacgc
    13141 ggcgaggagc agcaggggcg cccaccacga atagccgaac agcaccagcg ccgacgcgac
    13201 gccggccagc aagagcacga cactgccggc gacgaagtcc atgttgatga acatcggcgg
    13261 cccggtcatg cccaggtcga agtcgcgcgc cgtggtcagg tcgttgctca agtcgccgcg
    13321 ctcgagatgg ccgatgccgt cgggctcgag ggtggccgtg agcagccgtt cgttgagttc
    13381 gttgctcagg cgatggccga gcagggtgcc gatcacctga tgcagcggtg gggccacctg
    13441 ggcggcgacg aagaggatcc cgaaggcggt cagcggcgtg gtcagatcgg aaccggcggc
    13501 gacggccccg accaaccagc cggtgcagac ggccagcgcc gccggcagca gggagcgcag
    13561 cgcgagcagt gcccaccaca cggcggagag gccgggcacg gctcgggtca tcgtgacgaa
    13621 gaagcgccac gacggccgct gcgtcaactg ggtccgtgcc atgtcgatga ccgtagggca
    13681 cggcgccgac aaccctctag aacatttggg aagcgcggac cggtcggcgt cgtcaccagc
    13741 cgcgtagtga ccgcaccgtc tcgatggtgt cggcctggtc ggcgctcttg tcatcccggt
    13801 agcgcagcac cctggcgaac cgcagcgcca taccgccggg gtagcgcgaa ctcttctgga
    13861 tgccgtcgaa agcgatctcg accacctgct cgggccggac gtggacggtg taggagtcgc
    13921 ggtgggtctc caactccagg aagcgctcgg tctgccaggc cagcatcgcg tcggtcatcc
    13981 ccttgaacgt cttgccgagc atgacgaatc cgccggtgtc cgggtcgcgc gcgccgaggt
    14041 ggatgttcga cagggtgccg cgccggcggc cgctgcccca ctcgacggcc aggacgacga
    14101 ggtcgagggt atgccgcggt ttgaccttga cccagcccgc gccgcgccgg cccgcggcat
    14161 acgggctggc ggggtccttg accatgacac cttcgtggcc ggccgcgact tgcgcggcga
    14221 agaaggactc ggcgtccgcg ggggagtcgg tggtgatccg gaccatgcgt gcttgctcgg
    14281 gcaagagcgc atcgaggcgc gagatccttt cggccagacg gtgatcgagc agatcctcgc
    14341 cgtcgaggtg gagcaggtcg aagaaggatg tcgcgagcgg ggcggtgcgg cgcagggcct
    14401 cggggtcggc gctggaggcg gtgcgggcgc cggttacctg gaagggcgcg gggcggccgt
    14461 cggggcgcat cgcgatggtc tcgccgtcga ggaccacccg gtcgcagggg agcgcggcga
    14521 cgagctcgac gacctcgggc agccggtcgg tgatctcgtc gagggagcgg gtgaagaggc
    14581 gcacgtcggc gccggccttg tgcgcctgca gccgtatgcc gtcgatcttc cgttcgatga
    14641 agacgtccgc cccatcccgg aatgcgcccg cgatgtccgg cgaactgccc gcgagcatcg
    14701 gacggagcgg gcgcaggacc tcgagggtca tcgccgcgag cgcgtcgaca ccgcccgaca
    14761 gggcggcttg cgcaaccggt gccgcgaagc cgctgagcat gaccgcccgc tgcacggccg
    14821 ccagcggcac gtccgcggcc ttggcgatgg ccgcgagcat cacgccgtcc tgcgcgccct
    14881 gccgcacgcc accgagcatg agtccgcgca ggtattcctg ttcgggcccc gtggcccggg
    14941 cgagcaggtg cgacaccact gcgcgtcgac tggcaaccga gcccgagccc cccgcggtgg
    15001 ccagggcgtc aaaggcggca tcgacatcca cgagggtcaa tgacgcgtcg gccgccggcg
    15061 ccggaaggtc cgacaatccg cgccagccca ccccgaggcg acgttgcggc agcacattgg
    15121 agaggtatgc ggccgccaga cccacctcct ccggaccggc ggcctggaga gcctcggcga
    15181 ccagggcgat cttggcgttg cgcgccgatg tggcgacgag ggcgttcgag gtcgcgacaa
    15241 ggtgagacaa ctgcataccg ccgattgtgt cctgtgccgc cgacacgcgc ccgagccatt
    15301 ccggcgtgag catcggggtg gcagtggtca gtcagcccgc tgcgccccgt cgcgaccccg
    15361 ctggttatgg gcccggactc gattgcggtt gccgcaatcg tcggcccggc accagcaacg
    15421 gttgcgctgc cgcgaagtgt cgacgaagac atctcgtggg cccacccttt ggcgccttcc
    15481 tgttcgctgc cgccgcctcg gtcgcctcct tcaccagcgc gggggctttc gctctggcgg
    15541 gtctggcggc gttcctgctt ccccagctcc tgcccactgc ggccgagcgg ggccacgagc
    15601 acgggaccgt cgccggtgtg ggccgtagca tcgccggcgg tggtcccact tctggcacca
    15661 cgacttgctg cgccgcgtgg cgttcatctc cgcgtcgatc aacgtcttca gcgccgcgac
    15721 cagggggttg ctggtcctgc tcgtgaccgg gccgctcggg gtgccgccgg aaatgttcgg
    15781 tctcttcatc gcggttcccg ccgcgggggc gatcgtcggg tccctcgcgg ccgagcgagt
    15841 cgtgcccgct gtcggtgggg gacccatcac ctggcttgcc gcactggtcc ccgccgccag
    15901 tttcgccgcc ctcgggctga cctcgaacgt gcccgtgatc ctcatcgccg agttcctggc
    15961 cgcggtcgcc agttcgctca accagatcgt ggtcagcacc ctgcgccaag ccgccgtccc
    16021 cgacgcactg ctcgggcgag tgacggccgc ctaccnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnctgcg ccaagccgcc gtccccgacg cactgctcgg gcgagtgacg
    16201 gccgcctacc agccgtcacc tcgacatttc gatgcgtccg gtctcgtcga agctgtcacg
    16261 cgagtgtccg aggggggact tgaaccccca tccccttgcg aggactagca cctcaagcta
    16321 gcgcgtctgc caattccgcc acccggacga ggtgtgatga ggcgctgggg agcgccgcac
    16381 cgaagggaga ggttagcacg cggttcgggt gcttccctaa ccggcgaggg agcacccgtg
    16441 taccgggcgt gggtaccctc gctgtcatgc aggtcctgga gccacgggag gtgccgctcg
    16501 gcgggctgcg ggcgatgctc gtgcgccgca cgctgccgca ccgcgaccgg tcgttcatcg
    16561 gtgcctggtg cttcgccgac cactacggcc cgacccgcgt cggcgcggac gggccgggca
    16621 tggacgtggc gccccatccg cataccggcc tgcaaaccgt ctcctggctc ttcgacgggg
    16681 agatcgagca ccgcgacagc ggcagcttcc atgcgctcgt gcgtccgggc gagatcaact
    16741 tgatgaccgc tggtcgcggc gtcgcgcact cggaggtctc gaccccgacg accgaggtgc
    16801 tgcacggcgt ccagctctgg gtcgccctgc ccgaggaggc ccgccacgtc gagcggacct
    16861 tcgagcacca cgccgccccg gtcatggagc tgcccgccgg cgccggcgcc gcccgcgtct
    16921 tcctcggcga actgcgcgac atcgccgcct cacccgtcca caccttcacg ccgctcctcg
    16981 gcgcgcaact cgacctcacc cccggcgccg aggtcacgat caccctcgac ccggcatacg
    17041 agcacggcgt gctcctcgac accggcgacc tgcgggtcga cggagccgaa ctgcataccg
    17101 gctcgctcgc gtgcgtcgac gccgggccta ccagcctgcg cctcgtcacc gaccagggtg
    17161 cgcggctgat cctgttgggc ggcaagccgt tcgaagaaga gatcgtcatg tggtggaact
    17221 tcgtcggccg cagccacgag gaggtcgcga ccctgcgggg ggagtgggag gcgggcagcg
    17281 aacgcttcgg cacagtgcag gggtatgccg gtgctcggcc ccgcatccca gccccaccgc
    17341 tgcccggtgg ccggctgcgg ccgcgtggcc gcctccgccg cgcgtgatgc aataggcggc
    17401 atgcctgcac ccttgcgcga tatcgccacc gcggcgaacg ccgacgaggt gctcgagcgc
    17461 ttcgaggagt gggcgagcgg gcgcggcatc cggctgtatc cggcgcagga ggaggccgtc
    17521 ctcaccctcc tcaccggggc caacgtcatc ctggccacgc cgaccgggtc ggggaagtcg
    17581 ctcgtcgcgg tcgccgcgca cgccacggcg atggcggcgg gggagcgcag ctggtacacc
    17641 gccccgatca aagcccttgt gtcggagaag ttcttcgacc tgatccagat cttcggtgcc
    17701 gacaacgtcg ggatgctcac gggcgacgcc gccgtcaacg ccgatgcgcc catcatctgc
    17761 gccacggccg aggtactcgc gaatcaagct ctgcgcgaac gcgatcgcgc cgacatcggt
    17821 ctcgtcgtca tggacgagtt ccacttctat gccgagccgg accgcgggtg ggcgtggcag
    17881 gtgccattgc tgctgctccc gcgcacccag ttcctcctga tgtccgccac cctgggcgac
    17941 acgaccttct tcgaggagga tctgacgcgc cgcaacggcc gcgacactgt cgcgatcacg
    18001 tcggccgagc gtccggtgcc gctgcacttc gactgggttc tcaccccgat ccacgagacg
    18061 atcgaggacc tgctgcacga agggcgcgcg ccgatctacg ttgtgcactt cacccagcag
    18121 gcggccgtcg agcgcgccca ggcgctgatg tcggtcaacg tctcgacgag cgccgagaag
    18181 gaggagatca agcggctcgt cggtggattc cggttcggca aggggttcgg tatgacgctc
    18241 aaacgccttg tcctgcatgg catcggcgtc caccacgcgg ggatgttgcc gaaataccgg
    18301 cgcctcgtcg agaccctcgc ccaggccggc ctgctcaagg tcgtgtgcgg caccgacacg
    18361 ctcggcgtcg ggatcaacgt cccgatccgc agcgtcctgc tcacgtcgct gaccaagtac
    18421 gacgggcgcc gccaacgggt gctccgcgcg cgggagttcc atcagatcgc cgggcgggcc
    18481 gggcgggccg gctacgacac ggaaggtttc gtcgtcgtcc aagcacccga gcacctcatc
    18541 gaaaaccatc gagccctgct caaggccggc gacgacccga agaagcgtcg caaagtgcag
    18601 cgcaagggtc cgccggaggg gcagctcagc tacaccgagg ggacatatga ccggctcgtc
    18661 gccgccccgc ccgaaccgct gcgctcccgc atgcgcgtca cgcacggact tgttctcaac
    18721 ctcctcgacg aggtcgaggg tcagggtgag gcgatgcgaa tcgtgttgga ggacaaccac
    18781 gaagaggctg tctcgcagtc ccggctcgtg gtccgcgcag cgcagatcat cgcctccctg
    18841 gaaaaggcgg acgtcgttgc gccggtgccc ggggccgagg gcttcgaggt caccgtgccg
    18901 gtgcccgacg cagccgaggt cctggacgag ccggacgaac aacgcgtcgc cgagctcgag
    18961 atcgaggccg gcgccgcggc cgacggcgtc gacgatcccg atgtcgagga ggtgaccagc
    19021 gccgacccgc tcgacgtgcg cgggacgctc gccaccgcat acgaaatcgt cgacgacctc
    19081 caggacggct ttgccctcaa ccagcccctc tcggccttcg ccctcgccgt cctggacgtc
    19141 ctcgacgaga ccgaacccga ctacgccctc gacgtcgtca gcgtcatcga ggcgaccctc
    19201 gaggacccac ggcaggtgct gatggcgcag cagttcgcgg cacggggcga ggcggtcgcc
    19261 cagatgaagg cggacggcct ggaatacgaa gaacggctcg agttgctcga agacgtcagc
    19321 tggccgaaac cgttgcagga gttgctgacc gacgcctttg cgatctatcg ccagagccac
    19381 ccgtgggtct acgaggtcga cctgtcgccc aagtccgtcg tccgcgagat gtacgagcac
    19441 gcgatgacct tcggcgagta cgtcgggcgc taccaactgg gccgggccga aggcatcctg
    19501 ttgcgttatc tctcggacgc ctatcgcgcg ctgcggcaga cggttccgct cggctatcgc
    19561 gatgacgagc tggacgacgt catcgaatgg ctcggcgaac tgatccgcca gaccgactcg
    19621 agtctggtcg atgagtggga gtccctgatg aaccccgccg acgcgggccc ggaggtgcga
    19681 ccggagacca gccgggcgct gtcggcgaac ccccgcgcgc tgcgcgtcat ggtccgcaag
    19741 gcgatgttcc ggcgcgtcga actccttgcg ctgcagcggt atgccgcgct ggctgccttg
    19801 gctggtccgc tcaccgaagc gcaatggcgc gaggcgggcg aggactattt cgccgaatac
    19861 gaccacctgg gcaccggggc ggacgcgcgt ggcccggcat acctgcggat cgaggaggaa
    19921 ccggaggagt gggtcgtgac ccagatcctc gaggaccccg acggcgatcg cgactggcgc
    19981 ctcacggcca ccctggacct ggccgagacc gacgccgccg gcgaaccggt ctggacacgc
    20041 atggacctcg ggcctgccac gaggtagggc caccgggcca tgccgtcaac ggccttcatt
    20101 ccctccggac gacccgcccg actgggctgg ctggagaacg gcgcatggtg ccagtccgcc
    20161 ccggccgtgt gaacccaacg ccttcatagg ctgcgcccaa gcgacttgtc cgggggtagg
    20221 cgtacagaac cgttccacgg gcgtctgcgt ccgcaaggat cgcgcgggca agcgggcgga
    20281 agtgaccctc gtggcgcggc cgggcaaggg cgtcgccgag cttcgcccca cctcgatagt
    20341 ccgtcatctt gcacgcggcg aacgatgagt gcctcgcact gacccagtag gaaatccccc
    20401 ggcgcgcacg ccggaagttg aggcagagcg ccgtgggtag atagccagcc aggcaagggc
    20461 atagcggtgg gcgagccctg cgggaagacg ctggaagtgc tggggtccgt cggttgtcat
    20521 catgacgtga gcatgaccat ggccactgac agcctccgcg cactgacccc ggcgccgcct
    20581 atggtttcgc catggaccta cccgtcatgc cgcccgtgca gccgatgctc gccaagtccg
    20641 cgggcggcat acccgacccg gcgaagttcg acggtggctt gagcttcgag ccgaagtggg
    20701 acgggttccg gtgcctggtc ttcaaggacg gcgacgaggt cgagctggcc tctcgcaaca
    20761 ccaagtccct agcccgctac ttcccggaag tcgttgccgc actgcgggat tgcctgcccg
    20821 agaaggtggt gctggacggt gagatcttcg tggccgtcgg ggatcggctg gagttcgagg
    20881 tgctgcagga gcgcatccac cccgccgcct cgcggatcaa gctgctcagc gagcagaccc
    20941 cggcgcaata cgtcgccttc gacctgctcg ccctcgccga ggaggacctc atggcgaagc
    21001 ccttcgccga gcgtcgcgcc gaactggtcg atgtcctgac cgcccccagc gacaccgtcc
    21061 acatcacccg gacgaccacc gaccccgcca tcgcgcagga atggttcgag accttcgagg
    21121 gcgccgggct cgacggggtg gtcgccaagc cgctgggcgc gccctatgtc cccaacgccc
    21181 gctcgatgat caagatcaag caggcccgca cggccgatgt cgtcctcgcc ggttatcggc
    21241 tgcacaagaa ctccaccgcg gccgagccac tgctgggctc actgctgctc gggctgtatg
    21301 acgacgaggg ccacctcgcg cacatcggtg tcagcgcgtc cttcaccgcc gccaaacgca
    21361 aaagccttct cgcagaactg aaaccactgg aggagggtgt cgacgatcat ccatggggcg
    21421 agtgggc
//
LOCUS       DAOPJG010000030        21332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2623_length_21332_cov_7.907506, whole genome shotgun sequence.
ACCESSION   DAOPJG010000030 DAOPJG010000000
VERSION     DAOPJG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 21332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21332
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2623_length_21332_cov_7.907506"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKU75_04630"
     CDS             complement(<1..551)
                     /locus_tag="PKU75_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPF80194.1"
                     /translation="MKRNAGTAIALALAAVATPGLVTSSAAAGATGPVVVDGAAQPVY
                     ASAPRDWIVKEAWVTVPVDSDRDGRPDRVHVNYARANDAPDRVPVVMAASPYFTGVNE
                     VPNHNVDHDLYYPDTRWAQRTDALARQGNSANGLHDPWTAAYWVPRGFGWVEVESLGT
                     GTSTGCPTTGGTNETLGPKAVID"
     gene            735..1043
                     /locus_tag="PKU75_04635"
     CDS             735..1043
                     /locus_tag="PKU75_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80195.1"
                     /translation="MSHISFRHASALTAVALIGAFGSGMGAQAEVAPADRAAVGGIIS
                     ITSPEPEVVIGHLRDQLGAVGLPIGPDDGTVFHFDGAGWSGIVTQSEATTTVNWSVAR
                     "
     gene            complement(1080..2852)
                     /locus_tag="PKU75_04640"
     CDS             complement(1080..2852)
                     /locus_tag="PKU75_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPF80196.1"
                     /translation="MPISKVLIANRGEIAVRIARACTDFGIGSVAVYAEPDRDALHVK
                     VADEAYALGGSTPGDSYLLQDKLIEIAQESGADAIHPGYGFLAENGPFAQKVIEAGLV
                     WIGPGPEAIDSLGDKVKARHIALEADAPLVPGTKDPVSGPDEVVEFAKEHGLPVAIKA
                     AYGGGGRGLKVARTMEEIPELYESAVREAVTAFGRGECFVERFLDRPRHVETQCLADQ
                     HGNVVVVSTRDCSLQRRNQKLVEEAPAPFLTEEQHTELLRASKAILKGAGYVGAGTCE
                     FLVGPEGDISFLEVNTRLQVEHPVTEEVTGVDLVREQFKIANGEALSFTDPEPHAHSF
                     EFRINGEDAGRGFLPQPGTVTKMTVPHGPGVRWDAGIVEGDTVAGAFDSMIAKLIVTG
                     ATRQQAIERSRRALAELHIEGMPTVTPFHRKVVADPDFAPADPQEKFKIHTRWIETEF
                     DNDIEPYAGPSGEVPEAEERHNVVVEVGGKRIEVSLPGGLSLGGGGTVGGAKKKAPRR
                     SSAAKSGQAASGDSLTAPMQGTIVKIAVEEGQEVAAGELILVLEAMKMEQPINAHKAG
                     TITGLAAQVGATVTNGQVLAEIKD"
     gene            2935..3780
                     /locus_tag="PKU75_04645"
     CDS             2935..3780
                     /locus_tag="PKU75_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPF80197.1"
                     /translation="MPVPTVDLSAPDGPVAIPQLGFGVWQVPDDEARTAIEIALRDGY
                     RHIDTARLYHNEEGTGAAIAASGIPRDQVFVTTKLWNDDQGRARTEAAFEGSLGRLGM
                     DYVDLFLIHWPAPAHGLYAETWEILLELRDSGRTRAAGVSNFQPAHLEAAHARTGEWP
                     ALNQIELHPYLQQKELRAFHAEHGIMTEAWSPLASGKKVMDDPVIRGIAEKHGCTPAQ
                     AIIAWHLAIGNVVIPKSVTPARIAENFAALDVTLDADDLAAIDGLDRGMRTGPHPDKF
                     SGDPQ"
     gene            3777..3923
                     /locus_tag="PKU75_04650"
     CDS             3777..3923
                     /locus_tag="PKU75_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80198.1"
                     /translation="MSAPPPPPPPPRRGMDRRKYPAYVSAAVAASSLVAFIVVAWLVY
                     AALT"
     gene            complement(3949..5403)
                     /locus_tag="PKU75_04655"
     CDS             complement(3949..5403)
                     /locus_tag="PKU75_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF80199.1"
                     /translation="MAAPPADAPTARLVAFGRALRAHGISTGTSDLVDAAAAAQVLGY
                     DDRERLRAGFASALLRRSSERRLFDDLFDVFFPSALGTRSVVAQDDPLPPAEDAAGRR
                     ARAAALRDELATALARRDRRAREEVAARTVAILGRLPNDSTLGAYSAAQALDLLAPQT
                     AIAAALERAQDAGIDVPGGSGDGSGGSGGAGRGLASRFTRDEMRSEIAEFRRAVETET
                     RRRNAEARGSERIARYAVRAPVDQTSFVLAGPDELAELRRAITPLTRKLATRLAARRR
                     SGRGAPIDIRRTLRRAMGTGGVPMRPAYQKRSTSRVDLVLLCDMSSSVAGFSRFTILL
                     MQALASQFRRVRIFGFVNVVDELTETIATAAPGADLSGNFSDTARMTKWHSNSDYGTA
                     FHDFADQHLDAVGHRTVVLILGDARTNNTDPGLDALHTINERAKAVFFLNPEPARQWS
                     SGDSVAHLYSDIVDMHECRNLAQLRHFVARLLPV"
     gene            complement(5411..6271)
                     /locus_tag="PKU75_04660"
     CDS             complement(5411..6271)
                     /locus_tag="PKU75_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPF80200.1"
                     /translation="MPEPRFASVDDVAARLTGVGYIASDAIATTVFLADRLGKPLLVE
                     GPAGVGKTELAKAVTEAVAGELIRLQCYEGVDEARALYEWNHAKQLLRITAEQGQAWD
                     ELRTDIFTEEFLLPRPLLKAVRARGETILLVDELDKADQEIEGLLLEVLGDFQVTVPE
                     LGTIRAERPPFVVLTSNATRELSEALRRRCLFLHIDYPDAELEQRIVRQHVPDLSADL
                     AESVVRLVNRLRMLKLRKSPSVAETVDWARTLIALGTDDMTPEHVRDNLGVLLKHQDD
                     IERASHALEL"
     gene            complement(6264..6518)
                     /locus_tag="PKU75_04665"
     CDS             complement(6264..6518)
                     /locus_tag="PKU75_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80201.1"
                     /translation="MSLQRLIAACGDGPLVIFFALPPAVTMKCCELLRHIELPDVTRL
                     ALEKPFAPTSPPRSATGHQPGTLLTGVVAPARPVTDWVRA"
     gene            6909..8669
                     /locus_tag="PKU75_04670"
     CDS             6909..8669
                     /locus_tag="PKU75_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF80202.1"
                     /translation="MADGMGAIPIEGGTAFRVWAPHADTVSVVGSFNDWDPAVNPLSA
                     EGNGHWYGEVPGAHAGHEYKFQIRNGQSELLRIDPYARQVTSSVGNGVIYDHGAFDWQ
                     GDDFRLPSHDDLVIYEMHIGSFYRPDGNHVGTLELAMERFDHLGALGVDLIQLMPVAE
                     FAGDFSWGYNPAHIFAVESAYGGPDAFKTFVREAHRRGIGVVLDVVYNHFGPSDLDLW
                     QFDGWSENDKGGIYFYNDDRSQTPWGDSRPDYGREEVRRFIHDNAMAWLRDYRLDGLR
                     FDMTPFISSVNGGEVDLPDGWSLMRWINTTVREQFPDCITIAEDMHDDPSITRTEEPG
                     AGFHAQWDPAFVHPVREAVTADWDGDRSLESVWGSITSSFNDDAFQRVIFTESHDEVA
                     NGRARVPEEIDENDPTGWNARKRSTLGAALVFTAPGIPMLFQGQEFLEGGWFRDDVPL
                     DWDQSKEFVGISRLYRDLIRLRRNGDGQSRGLRGRGVGLLVGDETAKVLAFHRWADGG
                     PGDDVVVAVNLSAHPAVGLAVPFPRPGGWSLRLSSDAQVYCSDFGDGGIGALEVGGDG
                     VAHVDIPAYSVLIYSQDQAG"
     gene            8675..10087
                     /locus_tag="PKU75_04675"
     CDS             8675..10087
                     /locus_tag="PKU75_04675"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HPF80203.1"
                     /translation="MGDAAQHYDLVVLGAGSGRVVLGPDLAHLRAAIVDHGPYGGTCL
                     NRGCIPSKMFLHVARAAAGVPDLARLGVDARVERVRWDDIRDRVFGRIDPNAAAGPAE
                     VRSAGVDAYVGTARFVAPGRLVIEEGDGVTEVTAERVVLATGARPVVPDVVANSGVPF
                     VTSDDVMRLPALPERLLVLGGGVIGVEMATLFCGMGVRVTVVDSVEALVPTLGADIAR
                     RFTDMARTRWALHLGAEVTAVEQGRDGIHLRLDDGTALTGDALLVATGRRPNSDGFGL
                     ETIGVDIDPDSGRVLVDDFGRTSADGVWALGDVCTERQLKHLANDQARTLRHNLLTPD
                     DLKPFRTKAIPSAIFSQPELAQVGYLLEEAQKEGHDAVAYTQRLSDTAYGWALEDTTS
                     VCTVVAERSTGVVLGAQLMAPNASSLIQPLIDACARGRPAREIATTQMWIHPAATELI
                     ENALLGVADAAEGDSSHGDL"
     gene            10074..10430
                     /locus_tag="PKU75_04680"
     CDS             10074..10430
                     /locus_tag="PKU75_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80204.1"
                     /translation="MATYDVNPAGVKKCKELIAKKRYVLDSEWGDAAPDADAENAFLD
                     KHSWDDYSGWHLGLTEGPGDETKARYAFGFGDFTKVHRSALIACVYRAAQWRHKKVEL
                     AAHQLLQEFDKAAGIE"
     gene            complement(10438..10860)
                     /locus_tag="PKU75_04685"
     CDS             complement(10438..10860)
                     /locus_tag="PKU75_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF80205.1"
                     /translation="MASEATPGDTWTIAQIADEFAITHRAVRHYEDLGLISPERRGTQ
                     RVYHRRDRTRLNLILRGKRLGFPLEEIRTIIDLYDAPRGKRSQLEYVLGQIDERRADL
                     EARRRDLEAALDELDTFESRCRADLGALKPTQRTSPTG"
     gene            10922..12106
                     /locus_tag="PKU75_04690"
     CDS             10922..12106
                     /locus_tag="PKU75_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF80206.1"
                     /translation="MFELSQHHEDFRAMVRDFAEREVAPYVAQWDREHHFPTDLVPKM
                     GELGLFGLVVPEEFGGSADPESGPFTALCVAIEELGRVDQSIGITLSAGVGLGINPIL
                     TYGSAAQKERFLPDLVAGRALAAFGLTEPDAGSDAGASRTRATLDGEEWVVNGAKAFI
                     TNSGTDITSVVTVTARTGDTKDGRPEISAIMIPSGTPGFEVAPAYDKLGWNISDTHGL
                     NFTNARVPQENLLGERGSGFKQFLKTLDDGRIAISALALGLITRMREEATAYANTRIA
                     FGRPIGANQGVSFQIADLAMMEEAGRLLTYKAAHLKGEQEQGRRSVAEVKQAAAIAKL
                     YTSEAAVTATRIATQIFGGNGFMEEYPVARFYRDAKILEIGEGTSQVQRMVIARNLGL
                     PS"
     gene            12103..13683
                     /locus_tag="PKU75_04695"
     CDS             12103..13683
                     /locus_tag="PKU75_04695"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPF80207.1"
                     /translation="MSTDATPDLGVDPNADPKVADVRARLHAAYDASATPPPAAADKL
                     AKQNKLYVRDRIALLVDEGSFVEDGRYANALAKGLPADGVVTGRGLVEGRPVVVIAND
                     PTVKAGSWGSRTVEKIVRATEMALREELPVFWFVDSAGARITDQVEMFPGRRGAGRIF
                     HNQVALSGKVPQICCLFGPSAAGGAYIPSFTDLIIMVEGNASMYLGSPRMAEMVVGEK
                     VSLEEMGGARMHCTVSGVGDLLAHDDTEAIEMARHYFSYVPQNWHTPAPSYHPEQPVE
                     PLTRHTVPERESVPFDIHDVIDGLVDEESFFEIKPLFAPELVVGFGRIEGETVGIVAN
                     NSAVKGGVLFTDSADKATRFIWMCDAYSIPLIYLADVPGFMIGSEVERGGIIRHGAKM
                     VSAVSSATVPQFCVVVRKAYGAGLYAMGGPGFGPDATIALPTARIAVMGPEAAVNAVY
                     ANKIAEIADPAEREAYVAERRREYEEDVDLERLAADLVLDQVVEPEDLRAELAHRLAY
                     AAHRDRHFATKRRDIPPV"
     gene            13830..15500
                     /locus_tag="PKU75_04700"
     CDS             13830..15500
                     /locus_tag="PKU75_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPF80208.1"
                     /translation="MAEQERDLTPLSPLGTGRKRSGPVRAQRPVYVDLLPPCNAGCPA
                     GENIQAWLALAKAGDTHGAWHQLTQDNPFPAIHGRVCYHPCETVCNRGLMDGSVSIHA
                     VERHLGDTAIAEGWSYAEPRMSTGKHVLVVGAGPSGLSAAYHLTRLGHRVTVRDNSQL
                     PGGMMRYGIPAYRLPRDVIDAEIDRLRRYGVAFEQNHTVTDLQEEMTGFDAAFVAVGA
                     HLSKRVDIPNGDASRVLDAVSFLRDVEEGGRSAITGRVAVYGGGNTAMDAARVARRLG
                     ADDTIIVYRRTREQMPAHEEEAADAEEEGIRINWLRTISEMGEDDLTVEIQELDENGR
                     PQPTGRFEKLEADTVILALGQESDTGFLKNIAGLTFDGDVVQVDPVTLMTGAPGIFAG
                     GDAVPSERTVTVGVGHGKKAANAIDAYLGGGKFLPAHKHPVADMDKLHLWYFGDHGRR
                     QQGELDPSARAEVFTEVVAGLTSEEARYEAGRCLSCGNCFECDGCLGSCPDDAIIKLG
                     KGHRYRFDYSKCTGCGTCFRQCPVHAIEMIGEHDRLPGNVFLAPGQEA"
     gene            15501..19082
                     /gene="nifJ"
                     /locus_tag="PKU75_04705"
     CDS             15501..19082
                     /gene="nifJ"
                     /locus_tag="PKU75_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPF80209.1"
                     /translation="MATADRVTIDGNEATVNVAYRLSELCAIYPITPSSTMAELADDW
                     AAKKRTNIFGTIPTVIEMQSEGGAAGALHGSLQGGALGTTFTASQGLLLMIPNMYKIA
                     GELTSAVIHVAARSLAAQGLSIFGDHQDVMAVRQTGFALLSSGSVQEAHDLALVAHAA
                     TLRTRVPFVHFFDGFRTSHELNTIDLLDDEVLKALIPESLVHEHRMRSLSPERPFVRG
                     TAQNPDVYFQARETVNPFYARTPGIVQECMDLLAEQVGRAYHLVEYAGDPEAERVVVV
                     MGSGGETVEETVNHLNASGEKVGVIRVRLYRPFPVAELVAALPRTAKVVGVLDRTKEP
                     GSNGEPLYLDAVAALSGAFADGTLESMPRIVGGRYGLSSKEFTPAMVAGVYAALAQER
                     PPTGFTIGIHDDVGGTSLDYDPAFDIEDDQTLRAVFFGLGSDGTVGANKNTIKILGED
                     PNRFAQAYFVYDSKKSGGSTISHLRFGPHPIKAPYLVNTAGFVGCHHFGLIDKLDVLG
                     LARPGGVLLLDAPMAAGEVWDALSRQAQEQIVAKDLQVYAIDAGTVARNAGLPGRTNT
                     VLQTCFFAISGVLPRDEAIEEIKKSIRKSYGRRGQEVVRRNEAAVDAALEHLHRIEVP
                     DEASSQRNPRPIVADDAPEFVKSVTAAMMGGRGDALPVSAMPVDGTYPSGTTKYEKRR
                     ISDLVAEWDPKGCIQCGNCAFVCPHSVIRSKFYPAVALDAAPESFESAPLNAAGLPES
                     RYTLQVYAEDCTGCGLCVEACPVTPIPGSSRKAINLTDVTDSLDQRIENVGFFETIPH
                     NERARVDFGTVRGAQFLEPLFEFSGACNGCGETPYLKLLSQLFGDRLTVANATGCSSI
                     YGGNLPTTPWARNAQGRGPAWSNSLFEDNAEFGLGLQMASDLHTQLAQQRLSAMREEI
                     GAALVDEILTAPQIRESELAAQRARVDEMLRRLEGVEGPMAEDLRSVADHLVRRSVWI
                     VGGDGWAYDIGSGGLDHVLASGRNVNILVMDTEVYSNTGGQSSKATPIAAVAKFASAG
                     KTIAKKDLALQAVAYGSVYVARVAFGADPQQTLRAFREAEAYDGTSIVIAYSHCIAHG
                     IEMSKGLEQQKRAVNSAHWPLMRYDPKVRERGQNPFFLDSPRPTIPLSDYIYKELRYR
                     MLKASAPEEAERLLEMAQQAVDQRWATYEEMATREAAQFLPDSRKKD"
     gene            19082..20101
                     /locus_tag="PKU75_04710"
     CDS             19082..20101
                     /locus_tag="PKU75_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPF80210.1"
                     /translation="MDLHTSYLGLPLRSPLLASASPLSQTADGVRELADAGVGAVVLY
                     SLFEEQIRLQELRDFEVSAMHEDSFGEATSYFPEVELGTDGATPYLKHLENCVAAVDI
                     PVIASLNGSSLDGWSDMAKRMESAGAAAIEVNPYAVPGNLNVSSSGIEERHVEIVQTV
                     KAAVGVPVAMKLSPFFSATGAMCRQLDEAGVDGLVLFNRFLQPDIDIETLEVHPAVSL
                     SAPNEAGLARTWVAILRGRVQASLAATTGVETGADVAKYLLAGADVVMTASALLRHGP
                     GYAAVLLAELAAWMERKQYATVDAFRGLLAVGTGVDASGYERAGYVAALTHAKETYGD
                     FLIRD"
     gene            complement(20172..20819)
                     /locus_tag="PKU75_04715"
     CDS             complement(20172..20819)
                     /locus_tag="PKU75_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HPF80211.1"
                     /translation="MPTTRLILASASPARRNLLRQVGIEASVLPSAVDEDALIADLRS
                     RFGDLAPEDLVMGLARAKAEDVASGRDALVLGCDSMLELDGEVHGKPADAAEATDRWR
                     RMRGKAGTLHTGHWLIDDRDDGSGGLVGATASTRVHFADLTDAEIAAYVATGEPLRVA
                     GAFTVDGLGGAFVTGIEGDHHNVVGLSLPLLRDLLAELGIAWYDLAPQGTSASRG"
     gene            20932..>21332
                     /locus_tag="PKU75_04720"
     CDS             20932..>21332
                     /locus_tag="PKU75_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80212.1"
                     /translation="MSSAADPSATAPRRWWHAARAFPRDHPWAWDLLTAALFWLVLGF
                     FVLLSLLTGPREGNDSLVPGLVVSSLHTAGVALRRRRPVTAFWLVVAGCVVQLVASDN
                     LFPTDAAVLVVAYALTRYSDDVRMRRVGLAV"
BASE COUNT         3317 a         7213 c         7470 g         3332 t
ORIGIN      
        1 cagtcgatga ccgctttggg gccgagggtc tcgttggtgc cgccggtcgt cgggcagccg
       61 gtcgacgtcc cggtgcccag cgactccacc tcgacccacc cgaaaccgcg ggggacccag
      121 tatgcggcgg tccaggggtc gtgcaagccg ttggccgaat tgccttggcg cgctagcgca
      181 tccgtgcgtt gtgcccaccg cgtgtcgggg tagtagaggt cgtggtcgac gttgtggttg
      241 ggcacctcgt tgaccccggt gaagtagggg ctggccgcca tgacgaccgg cacccggtcg
      301 ggggcgtcat tggcccgcgc gtagttgacg tgcacgcggt ccgggcggcc atcgcggtcg
      361 gagtccaccg gaaccgtcac ccaggcctcc ttgacgatcc agtcgcgcgg agcactggca
      421 tacaccggct gcgccgcgcc atccacaacc accggccccg tcgctcccgc cgcggccgac
      481 gacgtgacaa gtccgggcgt cgcgacggct gccagcgcga gggcgatggc ggtcccggcg
      541 ttgcgtttca tgacttcccc ttcacgcccg cccaccctaa cggctggccc ccgtgggccg
      601 cgccggcccg agtatgacga cggcagtccg tcgatggatt catcttggcg gaagggccaa
      661 ctcttggacg agctccggcg tctacaaggt gtccgcgcgc ctgcgtcggg caggcgcctc
      721 agggaggcgg aaccatgtcg cacatcagtt ttcgtcacgc ttcggcactc acagcggtcg
      781 cgctgatcgg ggcattcggc tccggtatgg gcgcccaggc cgaggtcgcc cccgctgacc
      841 gcgcggcggt gggcgggatc atctcgataa ccagccccga accggaggtc gtgatcggcc
      901 acctgcgtga ccaactcggc gcggtgggtc tgcccatcgg gcccgatgac ggcaccgtct
      961 tccacttcga tggtgctggc tggagcggca tcgtcactca gagcgaggcg acgacgaccg
     1021 tcaactggtc cgtcgcgcgc tgaggtgacc ggccagcacg ccagggcgcg agaagcggat
     1081 cagtccttga tctcggcgag cacctggccg ttggtgaccg tggcgccgac ctgagcagcc
     1141 aggccggtga tggtgccggc cttgtgggcg ttgatcggct gttccatctt catggcctcg
     1201 aggacgagga tcagttcgcc ggcggcgacc tcctggccct cctcgacggc gatcttgacg
     1261 atcgtgccct gcatcggcgc ggtgagggag tcgcccgagg cggcctgccc gctcttggcc
     1321 gccgacgagc ggcgcggagc cttcttcttg gcaccaccga ccgtgccgcc accaccgagg
     1381 gagagcccac ccggcagcga gacctcgatg cgcttgccgc cgacttcgac gacgacgttg
     1441 tgccgctcct ccgcctcggg gacctcgccg gacggcccgg catacggctc gatgtcgttg
     1501 tcgaactcgg tctcgatcca gcgggtgtgg atcttgaact tctcctgcgg gtcggccggg
     1561 gcgaagtcgg ggtcggcgac gaccttgcgg tggaagggcg tgacagtggg catcccctcg
     1621 atgtgcagtt cggccagcgc ccggcgggac cgctcgatgg cctgctgccg ggtggcgccg
     1681 gtcacgatga gcttggcgat catcgagtcg aacgcgccgg cgaccgtgtc accctcgacg
     1741 atgcccgcgt cccagcgcac tccgggaccg tgcggcaccg tcatcttcgt cacggtgccc
     1801 ggctgcggca ggaagccgcg gccggcgtcc tcgccgttga tccggaactc gaaggagtgg
     1861 gcgtgcggct cggggtcggt gaaggagagg gcctcgccat tggcgatctt gaactgctcg
     1921 cggaccaggt cgacgccggt gacctcctcg gtgaccgggt gctcgacctg caggcgggtg
     1981 ttgacctcga ggaaggagat gtcgccttcg gggccgacga ggaactcaca ggtgccggca
     2041 ccgacatacc cagcgccctt gaggatcgcc ttcgaggcgc gcagcagttc ggtgtgctgc
     2101 tcctcggtga ggaagggtgc gggggcctcc tcgacgagct tctggttgcg ccgctgcagc
     2161 gagcagtcgc gggtggagac gaccacgacg ttgccgtgct ggtctgcgag acactgggtt
     2221 tcgacgtgcc gtggacggtc gaggaagcgc tcgacgaagc actcgccgcg gccgaacgcc
     2281 gtcacggcct cgcggaccgc gctctcgtag agctcgggga tctcctccat ggtgcgggcg
     2341 accttcagac cgcggccgcc accgccatac gcagccttga tcgcgaccgg cagcccgtgc
     2401 tccttcgcga actcgacgac ctcgtccggt ccgctcacgg ggtccttggt gccgggcacg
     2461 aggggagcgt cggcctcgag ggcgatgtgc cgggccttga ccttgtcgcc gagagaatcg
     2521 atggcctcgg ggccggggcc gatccagacg aggccggctt cgatcacctt ctgtgcgaac
     2581 gggccgttct cggccaggaa gccgtagccg gggtggatcg catcggcgcc cgactcctgg
     2641 gcgatctcga tcagcttgtc ctgcagcagg taggagtcgc cgggggtgct gccgccgagg
     2701 gcgtaggcct catccgccac cttgacgtgc aaggcgtcgc ggtccggctc ggcgtatacg
     2761 gcgaccgatc cgatcccgaa gtccgtgcag gcgcgggcga tgcggacggc gatctcgccg
     2821 cggttggcga tgaggacttt gctgatcggc atacgagcga gcctagcgct ccgcccgaat
     2881 ccggccaccg ctcgttccgg cctggggagg tgaggtcggt cataaggtgg tcctatgccg
     2941 gttcccacag tcgacctgtc cgcccccgat ggccccgtcg ccatcccgca actcggcttc
     3001 ggtgtctggc aggtgcccga cgacgaggcg cgcaccgcga tcgagatcgc cctgcgcgac
     3061 gggtatcgcc acatcgacac cgcgcggctc tatcacaacg aagagggcac gggagcagcg
     3121 atcgcggcga gcggcatacc gcgcgatcag gtcttcgtga ccaccaagct gtggaacgac
     3181 gatcagggcc gcgcgcgcac cgaggccgcc ttcgaaggct cgctgggccg gctcggcatg
     3241 gactacgtcg acctcttcct catccactgg cccgcgccgg cccacgggtt gtatgccgag
     3301 acgtgggaga tcctgctgga gttgcgcgac agcgggcgca cccgggccgc cggggtgtcg
     3361 aacttccagc ccgctcatct cgaggccgcg cacgcgcgca cgggggagtg gccggcgctc
     3421 aaccagatcg agttgcaccc ctatctgcag cagaaggagc tgcgggcctt ccacgccgag
     3481 cacggcatca tgacggaggc gtggagcccg ctggcttccg gcaagaaggt catggatgac
     3541 ccggtcatcc ggggcatcgc cgagaagcac gggtgcactc ccgcgcaggc aatcatcgcc
     3601 tggcatctgg cgatcggcaa tgtcgtcatc cccaagtcgg tgacgcccgc ccggatcgcg
     3661 gagaacttcg cggccctgga cgtcacgctc gacgcggacg acctggcggc catcgacggc
     3721 ctcgaccggg ggatgcgcac cgggccgcac cccgacaagt tcagcggcga cccgcaatga
     3781 gcgcgcctcc gcccccgcca cccccgcccc ggcgcgggat ggaccgccgc aagtatccgg
     3841 cctatgtgtc cgccgccgtc gccgcctcgt cgctggtggc cttcatcgtc gtcgcctggc
     3901 tggtgtatgc cgcgctgacc tagacgcccg cccccggact gggttagttc agaccggtag
     3961 cagcctggcg acgaagtggc gcagctgggc gaggttgcgg cactcgtgca tgtcgacgat
     4021 gtcgctgtag agatgcgcca ccgagtcgcc ggacgaccac tgacgggccg gctcggggtt
     4081 gaggaagaag acggccttgg cgcgctcgtt gatggtgtgc agcgcgtcca ggccggggtc
     4141 ggtgttgttg gtgcgggcgt cgccgaggat gagcacaacg gtgcggtggc cgacggcgtc
     4201 gaggtgctgg tcggcaaagt cgtggaaggc ggtgccgtag tcggagttgc tgtgccactt
     4261 ggtcattcgg gcggtgtcgg agaaattgcc ggagagatcg gcgccgggcg cggcggtggc
     4321 gatcgtctcc gtcaactcgt cgacgacgtt gacgaacccg aagatccgga cccggcggaa
     4381 ctggctcgcc aaggcctgca tgagcaggat cgtgaagcgg gagaagcccg cgaccgagct
     4441 ggacatgtcg cacagcagca ccaggtcgac ccggctcgtg ctgcgtttct ggtatgccgg
     4501 tcgcatcggc accccgccgg tccccatcgc gcggcgcagg gtgcgccgga tgtcgatggg
     4561 ggcgccgcgt ccggaccggc ggcgggcggc gagccgggtc gcgagtttgc gggtaagggg
     4621 agtgatggcg cgccgcagtt cggcgagttc gtcggggccg gcgaggacga acgaggtctg
     4681 gtcgacgggg gcgcgcacgg cgtagcgggc gattcgttcg gagccgcggg cctcggcatt
     4741 gcgccgacgg gtctcggtct cgacggcacg acggaattcg gctatctccg aacgcatttc
     4801 gtcacgcgtg aagcggctgg ccaggccgcg tccggccccg ccgctgccgc cggagccgtc
     4861 gcccgaacct ccgggcacgt cgatgccggc gtcctgggcg cgctcgagcg cggcggcgat
     4921 cgcggtctgg ggcgcgagca ggtccagggc ttgcgctgcg gagtaggcgc cgagggtcga
     4981 gtcgttcggc aaccgtccca ggatggcgac ggtgcgcgcc gctacctcct cacgtgcccg
     5041 ccggtcgcgg cgagcgagag cggttgccag ttcgtcgcgg agggcggccg cccgggcgcg
     5101 ccgaccggcg gcatcctcag caggcggcag cggatcgtct tgcgccacaa cggatctcgt
     5161 gccgagggcg gaggggaaga agacgtcgaa gaggtcgtcg aagagccggc gctcgctgct
     5221 gcgacgcagc aacgcggagg cgaacccggc ccgcaggcgc tcccggtcgt cataccccag
     5281 cacctgcgcg gctgcggcgg cgtcgacgag atcggaggtt ccggtcgaga tgccgtgcgc
     5341 gcgcaacgcc cgcccgaacg cgaccagtcg cgcggtcggc gcatcggcgg ggggcgcggc
     5401 cataccgccc tcacaactcc agggcgtggc tcgcccgctc gatgtcgtcc tgatgcttga
     5461 ggaggacacc gaggttgtcg cggacatgct ccggcgtcat gtcgtcggtg ccgagggcga
     5521 tcagggtgcg ggcccagtcg acggtctcgg cgaccgacgg cgacttgcgc agcttgagca
     5581 tgcgaagccg attgacaagg cggacaacgg attcggcgag gtcggcggag aggtccggca
     5641 cgtgctggcg cacgatgcgc tgctcgagtt cggcgtcggg atagtcgatg tgcaggaaga
     5701 ggcagcggcg gcgcagagcc tcggagagct cgcgggtcgc attggaggtc aggacgacga
     5761 agggcggccg ctcggcgcgg atcgtgccca gctcgggcac ggtcacctgg aagtcgccga
     5821 gcacctccag cagcaggccc tcgatctcct ggtcggcctt gtcgagttcg tcgacgagca
     5881 ggattgtctc gccccgggcg cgcacggcct tgagcagggg ccgggggagg aggaactcct
     5941 cggtgaagat gtcggtgcgc aactcatccc aggcctgacc ctgttcggcg gtgatccgca
     6001 gcagctgctt ggcgtggttc cactcgtaga gcgcgcgggc ttcatcgacg ccctcgtagc
     6061 actggagccg gatcagttct cccgcaacgg cttcggtgac cgccttggca agttcggtct
     6121 ttcccacccc cgccggaccc tcgacgagca ggggcttgcc gagccggtcg gcgaggaaga
     6181 cggtcgtggc aatggcgtcg gaggcgatgt aaccgacacc cgtgaggcgg gcggcgacat
     6241 catcgacaga ggcgaagcgt ggctcaggca cgcacccagt ctgtcaccgg cctcgcgggc
     6301 gcaacgacgc cagtcagtag cgttcccggc tggtggccag tcgcgcttcg cggtggcgag
     6361 gtcggtgcga acggcttctc gagcgccagt cgcgtgacgt cgggaagttc gatgtgccgc
     6421 aacagttcgc agcacttcat cgtcaccgcc ggaggcagcg cgaagaagat gacgagcggc
     6481 ccgtcgccgc aggcggcgat aaggcgctgc agggacaccg ggtcgaggag tcggccttga
     6541 tgtatgccgt gtccttgctc gccgcgcggg tgaccgtctg cggggcctgg aggtggcgaa
     6601 ggagtccttg atgcgggact tccattcggc aggggtcatc tcgacccggt ccgatccgac
     6661 gatgcgcaac gtccggtccc gctcggccat gagcaaggtg gcgataccgg gcagcagcag
     6721 ccgatgggtc agttcaccgc ccgcgccgag gatgagcaac gtggtcgccg gtttcgtcgg
     6781 cgtggccatg gtgtcgatcg ttgcacgaac aaggcacccg ggggaccttc ccggctcctc
     6841 cgagtgggtt gtgcggttac agtgcaaacg gcccgatcgc cgggtcgatg gcgatccgag
     6901 gagtgcgaat ggccgacggc atgggtgcga tcccgatcga ggggggcact gccttccgcg
     6961 tgtgggcgcc gcacgcggac accgtgtccg tggtggggtc cttcaacgac tgggacccgg
     7021 cggtcaaccc gctcagcgcc gagggcaacg ggcattggta cggggaggtg ccgggtgctc
     7081 acgcaggcca cgaatacaag ttccagatcc gcaacgggca gagcgagctc ctgcggatcg
     7141 acccgtatgc ccggcaggtc accagctctg tcggcaatgg cgtgatctac gaccacggcg
     7201 ctttcgattg gcagggcgac gactttcgcc tcccctccca cgacgatctc gtgatctacg
     7261 agatgcacat cggatcgttc tatcgtcccg acggcaacca cgtcggcacc ctcgagttgg
     7321 cgatggaacg cttcgaccat ctcggcgccc tcggggtcga cctgatccag ttgatgcccg
     7381 tcgccgagtt cgccggcgac ttctcgtggg gctacaaccc ggcgcacatc ttcgccgtgg
     7441 agtccgccta cggcggcccc gacgcgttca agacctttgt gcgagaagcg catcggcgtg
     7501 gaataggtgt cgtcctggac gtggtctaca accacttcgg cccgagcgac ctcgacctgt
     7561 ggcagttcga cgggtggagc gagaacgaca agggcggcat ctacttctac aacgacgatc
     7621 ggtcacagac cccgtggggc gacagccggc cggattacgg ccgcgaggag gtgcggcgct
     7681 tcatccacga caacgcgatg gcctggctgc gggactaccg tctcgacggg ctgcgtttcg
     7741 acatgactcc gttcatcagc tccgtgaacg gcggcgaggt cgacctgccg gacgggtgga
     7801 gtctgatgcg ctggatcaac acgactgtgc gcgaacagtt tccggattgc atcacgatcg
     7861 ccgaagacat gcacgacgat ccgagcatca cgcggaccga agaacccgga gcgggcttcc
     7921 acgcgcaatg ggatccggcg ttcgtccacc ccgtgcggga ggccgtcacg gccgactggg
     7981 acggggaccg gtcgctggag tccgtgtggg gatcgatcac gtcgagtttc aacgacgacg
     8041 ccttccagcg ggtcatcttc accgagtccc acgacgaagt cgccaacggc cgggccagag
     8101 tgccggagga gatcgacgag aacgatccga ccgggtggaa cgcccggaag cggtccacgc
     8161 tcggtgcggc gctcgtcttc acagcgcccg gaatcccgat gctgttccag ggccaggagt
     8221 tcctcgaggg cggctggttc cgcgacgatg tgccactgga ctgggatcag agcaaggagt
     8281 tcgtcggcat cagccgcttg tatcgcgatc tgatccgcct gcggcgcaac ggcgacgggc
     8341 agtcccgcgg gctgagggga cgcggcgtgg gtctgctcgt cggtgacgag accgccaagg
     8401 tgctggcctt ccaccgctgg gcggacggcg gcccgggcga cgacgtcgtc gtcgccgtga
     8461 acctgtcggc gcacccggcc gtcggcctcg cggtgccctt cccgcggccg ggcgggtgga
     8521 gtctgcggct gagcagtgac gcccaggtct actgcagcga cttcggcgac ggcgggatcg
     8581 gtgcgctgga ggtcggcggc gacggcgtcg cgcacgtgga tattccggcc tattcggtgc
     8641 tcatctacag ccaggaccag gcaggctgag ccccatgggg gacgccgcgc agcactacga
     8701 cctcgtcgtc ctgggcgcgg ggtccgggcg cgtggtgctc gggccggatc ttgcgcacct
     8761 gcgggccgcc atcgtcgatc acgggccgta tggcggcacc tgcctcaaca ggggctgcat
     8821 acccagcaag atgttcttgc acgtcgcgcg ggccgcggcc ggcgtgcccg atctcgcccg
     8881 gctcggagtc gacgcccgag tcgagagggt gcgctgggac gacatccggg accgggtttt
     8941 cggccggatc gatccgaatg ccgctgccgg ccccgccgag gtgcgctcgg ccggcgttga
     9001 tgcgtatgtc ggcacggccc gcttcgtcgc gcccggtcgc ctcgtcatcg aggaggggga
     9061 tggtgtcacg gaggtcacgg ccgagcgggt cgtcctggcc accggcgctc ggcccgtcgt
     9121 cccggacgtc gtggcgaaca gcggcgtgcc cttcgtgacg tcggacgacg tgatgcggct
     9181 cccggccctg ccggagcgtc tcctcgtcct cggcggggga gtcatcggcg tcgagatggc
     9241 aactctcttc tgcggcatgg gagtccgggt cacggtcgtc gactccgtcg aggccctggt
     9301 ccccacgttg ggggcggaca tcgcgcgccg gttcacggac atggcccgca cccggtgggc
     9361 cctccatctc ggcgccgagg tgacggcggt ggagcaggga cgcgacggca tccacctgcg
     9421 cctcgacgac gggacggcgt tgaccggtga cgccctgctg gtcgccaccg gccgtcggcc
     9481 gaacagcgac ggcttcgggc tcgaaacgat cggggtcgac atcgatccgg actcgggccg
     9541 ggtgctcgtc gacgacttcg ggcgcacgag cgcggacggc gtgtgggccc tgggcgacgt
     9601 ctgcaccgaa cgtcagctga agcacctggc gaacgaccag gcacgcacgc tacgtcacaa
     9661 cctgcttacg cccgacgatc tgaaaccctt ccgcaccaag gcaattccct cggcgatctt
     9721 ctctcagccc gagctcgccc aggtgggcta cctgctcgag gaggcccaga aggagggtca
     9781 cgacgcggtg gcgtacacac agcggctgag cgacacggca tacggctggg ccctggagga
     9841 cacgacatcg gtgtgcaccg tcgtcgcgga gcggagcacc ggcgtcgtgt tgggcgccca
     9901 actcatggcg cccaacgcat catcgctgat ccaaccgctg atcgacgcct gcgcgcgggg
     9961 acggccggcg cgcgagatcg cgacgacaca gatgtggatc cacccggccg cgacggaact
    10021 gatcgagaac gctctgctcg gtgttgcgga tgcggcagaa ggagattcga gccatggcga
    10081 cctatgacgt caacccggcc ggggtgaaga agtgcaagga actcatcgcg aagaagcgct
    10141 atgtcctcga ctccgaatgg ggcgacgccg cacccgacgc agacgctgag aacgcctttc
    10201 tcgacaagca ctcatgggac gactacagcg ggtggcacct cggcctgacc gaagggcccg
    10261 gcgacgagac caaggcccgc tacgcgttcg gcttcggcga cttcaccaag gtgcatcgct
    10321 ccgccctgat cgcctgcgtg tatcgcgcgg cgcaatggcg gcacaagaag gtcgaactcg
    10381 ccgcccacca gctgctccag gagttcgaca aggcagccgg gatcgagtaa ccgcccatca
    10441 ccctgttggc gaggtccgct gcgtgggctt gagggcgccg aggtcggccc ggcatcggga
    10501 ttcgaaggtg tcgagctcgt cgagagccgc ctccaggtcg cggcggcggg cttccaggtc
    10561 ggcgcgacgc tcgtcgatct gccccaggac gtactcgagt tgactgcgct tgccgcgcgg
    10621 ggcgtcatac aggtcgatga tcgtgcggat ctcctcgagc gggaagccga gccgcttgcc
    10681 gcgcaggatg agattgagcc gcgtgcgatc ccggcggtgg tagacccgct gcgtgccgcg
    10741 gcgttcgggg gagatcaggc cgaggtcctc gtagtgacgc accgcgcgat gggtgatcgc
    10801 gaactcgtcg gcgatctgcg cgatcgtcca cgtgtcaccc ggtgtcgcct cgcttgccat
    10861 gacccgatat cctgctttac gtttacgtca acgtcaagca cgcgacccgg ggagcccctg
    10921 catgttcgag ttgagccagc accacgagga tttccgcgcc atggtgcgcg atttcgcgga
    10981 gcgcgaggtc gcaccgtatg tcgcgcaatg ggatcgcgag caccacttcc cgaccgatct
    11041 ggtccccaag atgggggagc tcggtctgtt cggactggtc gtgccggagg agttcggggg
    11101 gagcgccgac ccggagtcgg ggccgttcac cgcgttgtgc gtcgccatcg aggaactcgg
    11161 ccgcgtcgac cagtcgatcg gcatcacgct ctcggctggg gtgggcctgg gcatcaaccc
    11221 gatcctgacg tatggcagtg cggcccagaa ggagcgcttc ctgccggatc tcgtggcggg
    11281 ccgggcgctg gcggccttcg gcctgaccga gccggacgcc ggttctgacg ccggggcctc
    11341 ccgcacccgc gccaccctcg acggcgagga gtgggtggtc aacggcgcca aggcgttcat
    11401 caccaactcc ggcaccgaca tcacctccgt cgtcacggtg acggcccgca cgggcgacac
    11461 caaggacggc aggccggaga tctcggccat catgatccct tccggcacac cgggattcga
    11521 ggtggcaccg gcatacgaca agctcggctg gaatatctcc gacacccacg ggctcaactt
    11581 caccaatgcc cgcgtgccgc aggagaacct cctcggtgag cgcggctccg ggttcaagca
    11641 gttcctcaag acgctcgacg acgggcggat cgccatcagc gccctggccc tcggactgat
    11701 cacgcggatg cgggaagagg ccacggccta cgccaacacc cgcatcgcct tcgggcggcc
    11761 gatcggtgcc aaccagggcg tctccttcca gatcgccgac ctggcgatga tggaggaggc
    11821 cggacggctg ctgacctaca aggcggcgca cctgaaaggc gagcaggagc agggccggcg
    11881 ctcggttgcc gaggtcaagc aggcggcggc gatcgccaag ctctacacct ccgaggccgc
    11941 ggtgaccgcc acccgcatcg cgacgcagat cttcggcggc aacgggttca tggaggaata
    12001 ccccgtcgcg cggttctatc gcgatgccaa gatcctcgag atcggtgagg ggacctcgca
    12061 ggtccagcgc atggtcatcg cgcgcaacct aggcttgccc tcgtgagcac cgacgccacg
    12121 cccgacctgg gcgtcgatcc caacgccgac cccaaggtcg cggacgtccg cgcccggctg
    12181 cacgcggcat acgacgcctc ggcgacgccg ccccccgctg cggcggacaa gctggccaag
    12241 cagaacaagc tgtatgtccg ggaccgcatc gccctgctgg tcgacgaggg cagcttcgtc
    12301 gaggacgggc gctacgccaa tgcgctcgcc aagggtctgc cggccgacgg tgtcgtgacc
    12361 gggcgcgggc tcgtggaggg ccggccggtc gtcgtcatcg ccaacgaccc gacggtcaag
    12421 gccggttcgt ggggctcgcg gacggtcgag aagatcgtgc gcgccacgga gatggcgctg
    12481 cgcgaagaat tgccggtctt ctggttcgtc gacagcgcgg gggcgcgcat caccgaccag
    12541 gtcgagatgt tcccggggcg ccgcggcgcc ggccgcatct tccacaacca ggtggccctc
    12601 tcgggcaagg tcccgcagat ctgctgcctc ttcggcccca gtgctgccgg gggtgcctac
    12661 atcccgagct tcaccgacct gatcatcatg gtcgagggca atgcctcgat gtatctcggc
    12721 agcccgcgta tggcggaaat ggttgtgggg gagaaggttt cacttgagga gatgggcggt
    12781 gcgcggatgc actgcaccgt ctccggtgtc ggtgacctgc tcgcgcacga cgacaccgag
    12841 gccatcgaaa tggcgcgcca ctacttctcg tatgtgccgc agaactggca caccccggcg
    12901 cccagctacc acccggagca gccggtcgag ccgctcacgc ggcataccgt ccccgaacgc
    12961 gaatcggtgc ccttcgacat ccacgacgtc atcgacgggc tcgtcgacga ggagtccttc
    13021 ttcgagatca agccgctgtt cgcgcccgaa ctggtcgtcg gattcggccg gatcgagggc
    13081 gagacggtgg gcatcgtcgc caacaactcc gcggtcaagg gcggggtgct cttcaccgac
    13141 agcgccgaca aggccacgcg cttcatctgg atgtgcgatg cctactcgat cccgctgatc
    13201 tatctcgccg acgtgccggg cttcatgatc ggctcggagg tcgagcgcgg aggcatcatc
    13261 cggcacggcg ccaagatggt ctcggccgtg tcctccgcca ccgtcccgca gttctgcgtc
    13321 gtcgtccgca aggcctacgg cgcaggcctg tatgcgatgg gtggccccgg cttcgggccc
    13381 gacgcgacca ttgcgctgcc caccgcgcgg atcgcggtca tgggcccgga ggcggccgtg
    13441 aatgcggtct atgcgaacaa gatcgcggag atcgccgatc cggccgagcg ggaggcctat
    13501 gtcgccgagc gccgccggga gtacgaggag gacgtagacc tcgagcgcct cgccgccgac
    13561 ctcgtcctcg accaggtcgt cgagcccgag gacctgcgag ccgaactcgc ccaccggctc
    13621 gcgtatgccg cccatcggga ccggcacttc gccaccaagc gccgcgacat cccgcccgtc
    13681 tgagcggcgc accgcccgca gctgtgccgc gagggaccgg ggcgttaggg ctgcctatcc
    13741 tgtgccgacc ggccgttttg actagggtcg taggcagtgg ggccgccagg ggcagtttcc
    13801 ccggcagcgg cggccggatg gaggcagcaa tggccgagca ggaacgcgat ctcactccgc
    13861 tatcgcccct gggcaccggg cgcaaacgct ccggcccggt gcgcgcgcag cggcccgtct
    13921 atgtcgatct gctcccgccc tgcaacgccg gctgccccgc cggtgagaac atccaggcct
    13981 ggctggcgct cgccaaggcc ggcgacaccc atggcgcctg gcatcagctc acccaggaca
    14041 accccttccc cgccatccac ggccgggttt gctatcaccc gtgcgaaacc gtgtgcaacc
    14101 gcgggctcat ggacggctcg gtgtccatcc acgccgtcga gcggcatctc ggtgacacgg
    14161 cgatcgcgga gggctggtcg tatgccgagc cacgcatgtc cacgggcaag cacgtcctcg
    14221 tcgtcggcgc gggaccgtcg ggcctgtccg cggcatacca cttgacccgg ctggggcacc
    14281 gagtcacggt gcgggacaac agccaactgc ccggcgggat gatgcgctac ggcatcccgg
    14341 cataccgcct gccgcgcgac gtcatcgacg ccgagatcga ccggctgcgg cgctacgggg
    14401 tcgccttcga gcagaaccac accgtcaccg atctccagga ggagatgacg ggcttcgacg
    14461 ccgcgttcgt cgccgtcggc gcgcacctgt ccaagcgcgt cgacatcccc aacggggacg
    14521 cgagccgggt gctcgacgcc gtctcgttcc tgcgcgatgt cgaggagggc gggcgcagcg
    14581 cgatcaccgg acgcgtggcg gtgtatggcg gtggcaacac ggccatggac gccgcgcgcg
    14641 tcgcccggcg ccttggcgcc gacgacacga tcatcgtcta tcgccgcact cgcgagcaga
    14701 tgccggcgca cgaagaggaa gcggccgacg cggaggagga gggcatccgc atcaactggt
    14761 tgcggaccat ctccgagatg ggggaggacg acctgaccgt cgagatccag gaactggacg
    14821 agaacggccg gccgcaaccc accgggcgat tcgagaagct cgaggccgac accgtcatcc
    14881 tcgccctggg ccaggagagc gacaccggct tcctgaagaa catcgcaggc ctgaccttcg
    14941 acggcgacgt ggtgcaggtc gacccggtca ccttgatgac cggggcgccg ggcatcttcg
    15001 ccggcggtga cgccgtgccg agcgagcgca ccgtcaccgt cggtgtgggg cacggcaaga
    15061 aggccgccaa cgccatcgac gcctatctcg ggggtggcaa attccttccg gcacacaagc
    15121 atccggttgc cgacatggac aagctgcact tgtggtactt cggtgaccac ggccggcggc
    15181 agcagggcga gctcgatccc tcggcccggg cggaggtgtt caccgaagtg gtggccgggc
    15241 tcacctccga ggaggcccgg tacgaggcgg gccgctgcct gtcgtgcggc aactgcttcg
    15301 aatgcgacgg ctgcctgggc tcgtgccccg atgacgcgat catcaagctg ggcaagggcc
    15361 accgctaccg cttcgactat tccaagtgca ccggctgcgg cacgtgcttc cgccagtgcc
    15421 cggtccacgc catcgagatg atcggcgagc acgatcgcct gccggggaac gtcttcctcg
    15481 caccgggcca ggaggcataa atggcaaccg ctgaccgcgt caccatcgac ggcaacgagg
    15541 ccaccgtcaa cgtcgcctat cggttgagcg agctgtgcgc gatctacccg atcacgccaa
    15601 gctcgacgat ggccgaactc gccgacgact gggcggccaa gaagcgcacc aacatcttcg
    15661 gcacgatccc gaccgtcatc gagatgcaga gcgagggcgg cgccgccggg gcactgcacg
    15721 ggtcgctgca gggtggggca ctggggacga ccttcacggc gtcgcagggc ctgctgttga
    15781 tgatcccgaa catgtacaag atcgccggtg agctcacgtc ggcggtgatc catgttgcgg
    15841 cgcgatccct tgcggcccaa gggctctcga tcttcggtga ccatcaggac gtcatggccg
    15901 tgcggcaaac cggctttgcg ctgctgtcgt ccgggtccgt ccaggaggcg catgatctcg
    15961 cgctcgtcgc gcacgcggcg accctgcgca cgcgggtgcc gttcgtccac ttcttcgacg
    16021 gtttccgcac ctcccacgaa ctgaacacga tcgacctgct cgacgatgag gtgctcaagg
    16081 cgctcatccc cgagtcgctc gtgcacgagc accgcatgcg ttcgctgtcg ccggagcggc
    16141 cgttcgtccg gggcaccgcc cagaatcccg acgtctactt ccaggcgcgc gagaccgtca
    16201 accccttcta tgcccgcact ccgggcatcg tccaggagtg catggatctg cttgccgagc
    16261 aggtcggccg tgcctatcac ctcgtcgagt acgccggtga ccccgaggcc gagcgggtcg
    16321 tcgtggtgat gggctccggg ggagagacgg tcgaggagac cgtcaaccac ctcaacgcca
    16381 gcggcgagaa ggtcggcgtc atccgggtcc gtctctatcg gcccttcccg gtcgctgaac
    16441 tggttgccgc cctaccccgc accgccaagg tcgtcggggt gctcgaccgg accaaggagc
    16501 ccggcagcaa cggcgaaccg ctctatctcg acgccgtcgc cgctctgtcg ggcgctttcg
    16561 ccgacggcac gctggagtcg atgccacgca tcgtcggcgg ccggtatggg ttgtccagca
    16621 aggaattcac gccggcgatg gtcgcggggg tgtatgccgc gctggcccag gagcgtccgc
    16681 cgacgggctt caccatcggt atccacgacg acgtgggcgg cacctcactc gactacgatc
    16741 cggcgttcga catcgaggac gaccagaccc tgcgcgcggt cttcttcggc ctcggctccg
    16801 acggcacggt gggagcgaac aagaacacca tcaagatcct cggcgaggac cccaaccgct
    16861 tcgcccaggc ctacttcgtc tacgactcca agaagtccgg cggcagcacg atctcgcatc
    16921 tgcgcttcgg gccgcacccg atcaaggcgc cctatctggt caacacggcc ggcttcgtcg
    16981 ggtgtcacca cttcgggctg atcgacaagc tcgacgtgct cgggctggcc cggcccggcg
    17041 gtgtcctgct gctcgatgcg ccgatggcgg ccggcgaggt ctgggacgcc ctgtcacggc
    17101 aggcgcagga gcagatcgtc gccaaggacc tgcaggtcta cgccatcgac gccggcaccg
    17161 tcgcccgcaa cgccgggctg cccggacgca ccaacaccgt gctgcagacc tgcttcttcg
    17221 ccatctccgg cgtgctgccc cgggacgagg cgatcgagga gatcaagaag tcgatccgca
    17281 agtcgtatgg ccggcgcggc caggaagtgg tgcgccgcaa cgaagccgct gtcgacgccg
    17341 cgctggagca cctgcaccgc atcgaggtgc ccgacgaggc gagttcgcag cgcaacccgc
    17401 gcccgatcgt cgccgacgac gccccggagt tcgtcaagtc ggtcactgcg gccatgatgg
    17461 gcggacgcgg cgacgccctg cccgtcagcg cgatgcctgt cgacggcacc tatccgagcg
    17521 gcacgaccaa atacgagaag cggcgcatct ccgaccttgt cgccgagtgg gaccccaagg
    17581 ggtgcatcca gtgcggcaac tgcgcattcg tgtgcccgca cagcgtgatt cgctcgaagt
    17641 tctatccggc ggtcgcactg gacgcggcgc ctgagagttt cgagtccgca ccgctcaatg
    17701 cggcgggcct gcccgagagc cgctacacgc tgcaggttta tgcggaggac tgcaccgggt
    17761 gcgggctgtg cgtcgaggcg tgcccggtca cgccgatacc cggctcgagc cgcaaggcga
    17821 tcaacctcac cgacgtgacc gactccctcg atcagcggat cgagaatgtc ggcttcttcg
    17881 agacgatccc gcacaacgag cgcgctcgcg tcgacttcgg cacggtccgc ggcgcgcagt
    17941 tcctcgaacc gctcttcgag ttctccggcg cctgcaacgg gtgcggtgag acgccatacc
    18001 tgaagctgct cagccaactc ttcggcgacc ggctcaccgt cgccaacgcc accggctgtt
    18061 cctccatcta cggcggcaac ctgccgacga ctccctgggc ccgcaacgcc caggggcgcg
    18121 ggccggcgtg gtccaacagc ctgttcgagg acaacgctga gttcgggctc ggcctgcaga
    18181 tggcgagcga cctgcatacg cagcttgcgc aacagcgcct gagcgccatg cgcgaggaga
    18241 tcggcgccgc actggtcgac gagatcctca cggcgccgca gatccgggag tccgagctcg
    18301 ccgcgcagcg cgcgcgggtc gacgagatgc tccggcggct ggagggcgtc gagggcccca
    18361 tggccgagga cctgcgcagc gtcgccgacc acctggtccg gcgcagcgtg tggatcgtcg
    18421 gcggcgacgg gtgggcctac gacatcgggt ccggcggcct ggaccacgtc ctcgccagcg
    18481 gccgcaatgt caacatcctc gtcatggaca ccgaggtcta ctccaacacc ggcggccagt
    18541 cctcaaaggc gactcccatt gcggccgtag cgaaattcgc ctcagccggc aagacgatcg
    18601 cgaagaagga cctcgcgctg caggccgtcg cctatggcag cgtctatgtc gcccgggtgg
    18661 ccttcggcgc cgacccgcag cagacgctgc gcgcgttccg cgaggccgag gcctatgacg
    18721 gcacgtcgat cgtcatcgcc tacagccact gcatcgcgca tggcatcgag atgagcaagg
    18781 ggttggagca gcagaagcgg gcggtcaact ccgcgcactg gccgttgatg cgatacgacc
    18841 cgaaggtccg tgagcgcggg cagaacccgt tcttcctcga cagcccgcgc ccgacgatcc
    18901 cgctctcgga ttacatctac aaggagctgc gctaccggat gctcaaggcg tcggcacctg
    18961 aggaggccga gcggctcctg gagatggcgc agcaggccgt cgatcagcgc tgggcgacct
    19021 acgaggagat ggcgacccgc gaggccgccc agttcctccc cgactcgcgg aagaaggact
    19081 gatggatctg cacacgtcct acctcggact gccgttgcgc agccccctgc tcgcttccgc
    19141 ctcgccgctg tcacagacgg cggacggggt tcgcgagctg gccgacgcag gcgtgggggc
    19201 agtggtgctg tattcgctct tcgaggagca gatccggctg caggagttgc gcgacttcga
    19261 ggtcagcgcg atgcacgagg actccttcgg ggaggcgacg tcctatttcc ccgaggtgga
    19321 actcgggacg gacggcgcga cgccctattt gaagcacctc gagaattgcg ttgcggccgt
    19381 ggacattccg gtcatcgcga gcctcaacgg gtcgagcctc gatggctggt ccgatatggc
    19441 caagcggatg gagtcggccg gcgcggcggc catcgaggtc aatccctatg ccgtgccggg
    19501 caatctcaac gtctccagtt caggcatcga ggagcgccac gtcgagatcg tccagacggt
    19561 gaaggcggcg gtcggcgtgc cggtggcgat gaagctgtcg cccttcttct ccgccacggg
    19621 ggcgatgtgc cggcagctgg acgaggcggg cgtcgacggg ctggtgctgt tcaaccgctt
    19681 cctgcagccc gatatcgaca tcgagaccct cgaggtccat ccggcggtgt cgctgtcggc
    19741 gccgaacgag gccggcctcg cgcgcacgtg ggtcgccatc ctgcgtggtc gcgtgcaggc
    19801 gtcgctggcg gcgaccactg gtgtcgagac gggtgccgac gtcgcgaagt acctcctcgc
    19861 cggcgcggac gtcgtcatga cggcctcagc cttgctgcgg cacggaccgg gttatgccgc
    19921 agtgcttctc gccgaactgg ccgcgtggat ggagcgcaaa cagtacgcca cggtcgacgc
    19981 cttccgcggc ctgctcgccg tcgggaccgg tgtcgacgcg tccggctacg agcgcgccgg
    20041 ctatgtcgcc gccctcactc acgccaagga gacctacggc gacttcctca tccgggactg
    20101 acaccaccgg agagccccaa ctcagcagag gttagtcaaa tcagcagacc tccctaggtg
    20161 tgctgatttg actaacctct gctagcagag gttccttggg gggccaggtc gtaccaggct
    20221 atgcctagct ctgccaacag gtctcgcagg agggggaggg agaggccgac gacattgtgg
    20281 tggtcgccct cgatgccggt gacgaaggcg ccgccgaggc cgtcgacggt gaaggccccg
    20341 gcgacccgca gcggctcgcc ggtggcgacg taggcggcga tctcggcgtc ggtcaggtcg
    20401 gcgaagtgca cgcgggtcga ggcggtggcg ccgacgagcc cgccggagcc gtcgtcacgg
    20461 tcgtcgatca gccagtgtcc ggtatgcagt gtccccgcct tcccgcgcat ccgccgccac
    20521 cggtcggtgg cctccgcggc gtcggcgggc ttgccgtgca cctcgccgtc gagctcgagc
    20581 atcgagtcgc agcccaggac aagggcgtcg cgcccgctcg ccacgtcctc ggccttcgcg
    20641 cgcgcgagcc ccatgaccag gtcctcgggc gcgaggtcac cgaagcggga ccgcaggtcg
    20701 gcgatgagcg cgtcctcgtc gaccgcgctg ggcaggacac tcgcctcgat cccgacctgg
    20761 cgcaacaggt tccgccgtgc gggcgaggcg gacgcgagga tgaggcgagt ggtcggcata
    20821 cgagaaatct atgccgagcg ccatccgtcc ccggacaacc ggggcaaccg acgcgccaac
    20881 tgcgaccgtg gtcggatgtg cgaggggtct gcgccccata ttgtcgagtg cgtgagttcc
    20941 gcggccgatc cctccgcgac ggcgccccga cgctggtggc acgccgctcg ggcgttcccg
    21001 cgcgaccacc cgtgggcgtg ggatctcctg acggccgcgc tcttctggct ggtcctgggc
    21061 ttcttcgtgc ttctgagcct gctgaccggg ccgcgggagg ggaacgactc gcttgtcccg
    21121 ggtctggtgg tctcgtccct gcacaccgcc ggcgtggccc tgcggcgtcg ccggccggtg
    21181 acggcgttct ggttggtcgt agccggatgc gtggtccagc tcgtcgcgtc ggacaacctc
    21241 ttcccgaccg acgcggcggt cctcgtcgtc gcgtacgcac tgacgcgcta ttcggacgat
    21301 gtgcggatgc gccgggtggg tctggccgta tg
//
LOCUS       DAOPJG010000031        21328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2625_length_21328_cov_8.238142, whole genome shotgun sequence.
ACCESSION   DAOPJG010000031 DAOPJG010000000
VERSION     DAOPJG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 21328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21328
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2625_length_21328_cov_8.238142"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKU75_04725"
     CDS             complement(<1..260)
                     /locus_tag="PKU75_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80213.1"
                     /translation="MSVLRTIRTRVLAVVLGAFAAVILIGLGALTFVLLSTLRAQAGS
                     TAQATAEWIAEVRSISCSPRRTRFLVVSTTRSPHSRATDRGTP"
     gene            complement(307..1197)
                     /locus_tag="PKU75_04730"
     CDS             complement(307..1197)
                     /locus_tag="PKU75_04730"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPF80214.1"
                     /translation="MTAPATPATAATESPAVKSQPVVMPPDATVGDAFRASLEACLAH
                     LDANEPLFLEGRHRDNLHQTRVSWRRARAAMSLFRPLFEHDEESMELKVRMREIVLPL
                     GPARDADVLLKQAEDEGWPYRTRRTLRGRRRRTYAVAVPLLRSPQWLEVKADLRAWLA
                     DPQWLDFASGLRDAPARDLTDAALQKRYARVAAAGPDLLTLQPHELHKVRIEGKKLRY
                     GCEFFADLYADAPGSLPNEFSTALGLMQDAFGEANDHATALHMLRAHGLPTDVLGPVH
                     GRAECVTTWAGVMALEPFWR"
     gene            complement(1206..1523)
                     /locus_tag="PKU75_04735"
     CDS             complement(1206..1523)
                     /locus_tag="PKU75_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80215.1"
                     /translation="MGLTPAAAAVYSAIRSTFGITNIGGVRPGDPGDHGTGRAVDVMI
                     SSSGQGDAVASYAIANMGSLGISYVIWQQRIWLAGSGGWRAMEDRGSPTANHMDHVHI
                     SVN"
     gene            1546..1833
                     /locus_tag="PKU75_04740"
     CDS             1546..1833
                     /locus_tag="PKU75_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80216.1"
                     /translation="MDELPPDELPPDDEFDELDEPDEEEEDVDDCVRAVVPDPVAGAC
                     AATGSGAGLGFGLATALTPSAPYAAPELSDTGARRSVVAARSARVVEREAR"
     gene            2136..2708
                     /locus_tag="PKU75_04745"
     CDS             2136..2708
                     /locus_tag="PKU75_04745"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HPF80217.1"
                     /translation="MGFTRAQLQGFRDHEVPDLIGPGLVLLFVGINPGLWTAATQTHF
                     ARPGNRFYPALLRAGIIERSIDPTAGMTDADRAYLLQRGIGITNIVRRATARADELSA
                     QEYAAGGRTLRATVREHRPRVVAVAGITAYRAAFGERRARPGRQPGDLVGAQLWVVPN
                     PSGLNAHETIDSLAQAYAAPARAAGLLPPT"
     gene            2772..3461
                     /locus_tag="PKU75_04750"
     CDS             2772..3461
                     /locus_tag="PKU75_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HPF80218.1"
                     /translation="MHVPDGFLDATTSLATAATAVAGVGLSLRGARRELSDFAAPLAG
                     LVAVFVFAAQMLNFPVGAGTSGHLLGGTLAAVLVGPATATLCLTVVLLIQALAFADGG
                     LSALGTNVTLMALVGVWAGYAVFLLARRVLPRRVASVPVAAAVGAFVSVPAAALTFTG
                     LYAVGGAAPIDLSSLATAMLGWHSLIGLGEAAITFLAVGAITATRPDLVHGARALAGV
                     QDLEIREGVRA"
     gene            3458..3793
                     /locus_tag="PKU75_04755"
     CDS             3458..3793
                     /locus_tag="PKU75_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HPF80219.1"
                     /translation="MNRDVSTRKRISTRTLLLWGLAVTLVLAGVVSFYASGHPDGLEF
                     VAEKLGFDSTAAGHAGGDSPVADYGVRGVENSRLSGGLAGLIGVLLVGIVMTGLVALL
                     RRRTPAKRD"
     gene            3795..4562
                     /gene="cbiQ"
                     /locus_tag="PKU75_04760"
     CDS             3795..4562
                     /gene="cbiQ"
                     /locus_tag="PKU75_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HPF80220.1"
                     /translation="MGAGHGHYLHYHGHSPVHRLPAHTKLVALLVFVGAVVATPRTAV
                     WAFAGYAALLLLAMARSGVPAAYLAKRMVVEVPFLVFALLMPFVATGPRVEVGPLRLS
                     EAGLWGAWGLLAKGTLGVGASVLLGSSTEPRDLVRALDRLRLPQPLVQIASFMVRYLE
                     VVLGELDRMRVARASRGFTARSVRHWPVLAATIGALFIRSYERGERVHLAMLSRGYAG
                     RLPFAAELTATRVQWVRALALPLIAAAGCVAAWMMAS"
     gene            4570..5325
                     /locus_tag="PKU75_04765"
     CDS             4570..5325
                     /locus_tag="PKU75_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80221.1"
                     /translation="MSTPVLDLRGVAFAYPDGHQALFGVDLHVHPGERVALLGPNGAG
                     KTTLVLHLNGILTAGAGSVTISGLPVTKDNLLEIRRRVGIVFQDPDDQLFMPTVREDV
                     AFGPANLGLAREAIDERVESALRSVGMLDFADRPPHHLSFGQRRRVAVATVLAMEPEI
                     LVLDEPSSNLDPASRRELAEIITALGVTVLMVTHDLPYALQLCERSVVLSDGVIAADG
                     ATADLLTDAELLARHRLELPYGFDPSVLVRSER"
     gene            5439..5621
                     /locus_tag="PKU75_04770"
     CDS             5439..5621
                     /locus_tag="PKU75_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80222.1"
                     /translation="MNTFAAIATYLLAAAGVALMLALATVPLVLDRDAERSDLAPAVP
                     LRPRPAAAPTATPRAA"
     gene            complement(5627..6016)
                     /locus_tag="PKU75_04775"
     CDS             complement(5627..6016)
                     /locus_tag="PKU75_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF80223.1"
                     /translation="MINAVHALIYSDDASATRAFFKDVLQLPFVTDDDNEGSDWLIFS
                     AGPCEIGVHPTGGEFEGESFSAPRHHQVSLMTTDVGATAQEITARGANILTGPSDMGF
                     GIGIEVEVPGADAILIYQPKHATAYDK"
     gene            complement(6013..6966)
                     /locus_tag="PKU75_04780"
     CDS             complement(6013..6966)
                     /locus_tag="PKU75_04780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPF80224.1"
                     /translation="MELRIFVEPQQGASYDDQLAVARRAEDCGYAAFFRSDHYLAMGG
                     SDGLPGPTDSWVTLAGLARETTTIRLGTLVSAATFRHPGLLAISVAQVDRMSGGRVDF
                     GLGAGWFEDEHAAYGIPFPPVAERFERLEEQLEIITGLWGTAIDEPYSFAGKHYSLSD
                     APALPKPATPQGPPIILGGKGVHKTPHLAARFADEFNVPFSPVAEVTQMYDRVADACA
                     QAGRDPGEIVRSAALVLACGRTDDELCGRATAIGRSIEDLTENGVAGSPAQCVDKLGT
                     YAEAGVQRVYLQVLDLSDLDHLDLVAERVVPQLGSQVGSET"
     gene            7086..7778
                     /locus_tag="PKU75_04785"
     CDS             7086..7778
                     /locus_tag="PKU75_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPF80225.1"
                     /translation="MDGGAALRERLEPLSGPVPLDRAGLVIAAVLAGVIIGWPPLWRR
                     VRLAVTLVHEAGHALVGVLCGRRFTGFVVSGDASGHAVTVGPVRGLGRIATTWAGYPA
                     PALAGCGLVWAAAHGYAAPLLALLLLGEVVLLPRIRSVMTGVVLIVALAATASAWWWR
                     DDTVQVHALTILGLVLLAGAWRGLAAVWQRGRAGDDHAVLRRLTGVPGVVWLLSWALV
                     CAAATLVAAGEL"
     gene            complement(7939..8958)
                     /locus_tag="PKU75_04790"
     CDS             complement(7939..8958)
                     /locus_tag="PKU75_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80226.1"
                     /translation="MSAAGLSVRDIVVRFGPVTAVDHIDLDVAPGEIIALLGASGSGK
                     SSLLRGIAGLEPIAGGSVAWDGLDLSRTPVHQRGFGVMFQDGQLFPHRDVGGNVAYGI
                     AGLPRAERQARVTQLLHLVGLAGYEHRAITTLSGGQAQRVALARSLAPRPRLLLLDEP
                     LSALDRALRERLVGMLRTTLKATETTAVYVTHDQDEAFAVADRVAVLIDGRIAQIGSP
                     GEVWRNPARADVAHFLGYSPLLPAGVAAGLGYPDPLGDKLVAVGPGGLVTDPAGRRLE
                     IVEQVDHRGYAAIRVRIPGDGTAVVRAPAQVPETAIAVRLDPAQCAVVAASPDGADGS
                     GAPTM"
     gene            complement(8955..10655)
                     /locus_tag="PKU75_04795"
     CDS             complement(8955..10655)
                     /locus_tag="PKU75_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPF80227.1"
                     /translation="MTTSTAPARADRRARGGPSWVWRCGWLLAAAIPLAFLAIFFAWP
                     VATLVGRGFASEDGSTLGAVAEVLTSARTWRILRTTLTLAVLGTVVSVALGVPGAYLL
                     YAVRFPGRGLLRALVTVPFVLPSVVVGVAFLSLLGTNGPLGFLGWEESLPAIVAALVF
                     FNYSVVVRTVGGLWSRLDPRAVEAARALEASPSRAFRTVTLPALLPAIASATTLVFLF
                     CASAFGIVMVLGGVRYSTIETEIWYQTTQLLDLPAAAALSITQLVVVTLALLATSWTS
                     ARQERALRLRGDASGEHTWSWRRDWAATLVTGATVLGVLALPMATMLLRSLRDREGWT
                     LDHYRALGTTVGRSLPVPVWTAIRNSLVVALEATAIALVLGTLVSLVVSRRPRDPLAR
                     KGLRALDAAFMLPLGVSAVTVGFGFLITLNRPPLDLRSSMLLIPVAQAIVALPLVVRS
                     ILPVLRAIDPRQREAAATLGAGPGRVLRTVDGPFLVRGLGLAAGFAMATSLGEFGATS
                     FLARLDRPTLPVVIFRLIGRPGEANFGAALAASVVLTTLTAVVMLLAERLRPAEASEL
                     "
     gene            complement(10664..11818)
                     /locus_tag="PKU75_04800"
     CDS             complement(10664..11818)
                     /locus_tag="PKU75_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HPF80228.1"
                     /translation="MTTLITSRRTRMPRAACAGAVAVALLAACSDGGSAGTSSSSSAA
                     SQSGSASASESASESASAELTRSDSLTVVTHDSFNLSDEVKKQFESDTGIKVTYVAPG
                     DAGTVVNQLVLTKDAPLGDVVFGVDNTFASRVETAGVLSAYPGAALPESAKAMLADDS
                     GMLTPIDYGDVCVNADKGWFADKKLDVPATLDDLAKPAYQDLLVVENPASSSPGLAFL
                     AATVGAKGENGYLDYWKSLKDNGVKVAKGWTEAYTGDFSGSSGKGPRPLVVSYATSPA
                     FEVDEGAKESRTVALLDTCFRQVEYAGILKGSQNEWGAQKFIDYLLTAGVQADIPGQM
                     YMYPVDSSVELPAEWVKFAPLATKPIAVDAATIGANRETWIKDWTAEVLG"
     regulatory      complement(11856..11960)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12027..12548)
                     /locus_tag="PKU75_04805"
     CDS             complement(12027..12548)
                     /locus_tag="PKU75_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPF80229.1"
                     /translation="MTEASSGNEASGGGRDESRVTAFVETFGATLTAAGMQRLPARVI
                     AVLLADDDGRMTSAEICEALHVSRAAVSGAITFLQAYGFVTKERERGTRRDVYVLHTD
                     TWHDVTLQKAAVIGYLVDQLQRGAEAVGLETAAGRRLRYSADFYAFVTEEMDRIIANW
                     EIRREQIRAQYLD"
     gene            12639..13541
                     /locus_tag="PKU75_04810"
     CDS             12639..13541
                     /locus_tag="PKU75_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80230.1"
                     /translation="MAHVIDIAGLVKTFGATRALDGLDLQVESGQVHGFLGPNGSGKS
                     TTIRVLLGLLRPDSGTARLLGGDPWADAPALHRRLAYVPGDVSLWPNLTGGEIIDLLG
                     RARGGLDQAKRADLIERFDLDPTKKGRSYSKGNRQKVALVAALASDVELLLLDEPTSG
                     LDPLMERVFTDIVRERRDQGVSALLSSHILSEVEKLCDHVSIIRAGRIVESGTLSQLR
                     HLTRTHISAELASPPTDLTAFPGAHDVAVDGDRFSCLVDSAQVGDLLARLTAHGVRSL
                     TATPPTLEELFLEHYDLPEEASSR"
     gene            13538..15178
                     /locus_tag="PKU75_04815"
     CDS             13538..15178
                     /locus_tag="PKU75_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80231.1"
                     /translation="MTTATLPAPATVRDAGPAAGTRVVLRRILRADRWFWLVWLVMLW
                     VTIPATASAYRETITDDAAGREVAAGLATNPTMRAMLGPPYDLLHVGGFTSWRVGGFV
                     AMMAAVMAGLGVIRATRADEEAGRTELLRAGSIGRHAPLLAALLVASGVCGLLALLVI
                     VSMIGLGTPASGAVALGLGIGVTGVAFAGVGAVAAQLTASARTARGIAMGSVGAAYLL
                     RAVADGTSEDAGMRWLGWLSPLEWSPYVRPYAGERWWVLALPIALAAGLIGFAFALEA
                     RRDHGAGLRPARPGPEHAAPALGSASALARRLDRGTAIWTLVGMGLFALVMGSMSNVF
                     DSIAEDPTLADRFRRMGAGATDLTDAFYVAMIGILVVLFALIGISLVERLRREEESGR
                     GELLLAGATSRSRVLSAFVLPALIVPIGVLILSGLLMALPAALRGDPQQLARIGGGAA
                     AQAPGIALIVGLAVAVHGLAPRLRLLPWLVTGWSLVVNWIGAVLGFPQRVLDATPFAY
                     LPRPPAEPMDWVPVVVESLLALGLLAAGWWGYARRDVT"
     gene            15210..15908
                     /locus_tag="PKU75_04820"
     CDS             15210..15908
                     /locus_tag="PKU75_04820"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPF80232.1"
                     /translation="MPQNEFGQPVGEPLPGWQARPPVIPVTLTGRHVRLEPLTAAHAG
                     ILYAPMVLESDPRIWTYYPYSPTMTRAEFDACIAVQLRNPAGAAHAIMAPDGAGLGFA
                     SYLRIDPPNGSVEVGGIVYSSALQRTTAATEAMILMARHVFHDLGYRRYEWKCDSCNE
                     PSKAAATRLGFTYEGRHRNAMVYKGRNRDTDWFSITGAEWPALSAAYDAWLAPGNFDA
                     AGAQRRSLGDLTRS"
     gene            complement(16001..16138)
                     /locus_tag="PKU75_04825"
     CDS             complement(16001..16138)
                     /locus_tag="PKU75_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80233.1"
                     /translation="MRRFTATLAFLLLGGLGLAPAAHADIAGPNSTSDGACIACWVTG
                     H"
     gene            complement(16138..16623)
                     /locus_tag="PKU75_04830"
     CDS             complement(16138..16623)
                     /locus_tag="PKU75_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80234.1"
                     /translation="MATMIRMSLTTEGSIMDIYPFRLAWKYPENLRLADESEVVLLEI
                     RSAIAAFNDSGSAGADGVLQHVSIQPDPADGFIDGLIFAVGTPDTVRRAATRLLRGIL
                     AQFTEAGELLEIQVPPPMATESAAMAVCASPRPLGAGEPGRLTSPRSGRQPGHDTKGL
                     T"
     gene            16667..17692
                     /locus_tag="PKU75_04835"
     CDS             16667..17692
                     /locus_tag="PKU75_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80235.1"
                     /translation="MTGSDGSGARPPVDGLAVLGLEPVTLDVYRQLLLRPEADLTTLA
                     EACGVGEPQARDALDALAATALLRPSQSHAGTWRPISPEVALRRLVAELEEAHAQQAV
                     RLARVRDDVLAFVTDYREEREARLARTVERLDGRDAVVSMLEELSAASTTEILSMSTS
                     RPAPEAVEQARDSDTATLARGVRSRTIYLEGVVDKAFRQSLFSFVSEGAEVRLAPTLP
                     VRMLIFDRAVAVIATDPDDIGAGAVVLRGGGVLIALVALFEMQWEAARAPFEVPTPEN
                     DVEVLSPMDTQVLRLLARGLKDEAVAHHLGVSVRTARRFIADLHERAGAGSRFELGVR
                     AKELGWL"
     gene            17876..18622
                     /locus_tag="PKU75_04840"
     CDS             17876..18622
                     /locus_tag="PKU75_04840"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HPF80236.1"
                     /translation="MRTSVRRPAATSIAVLAAVSLSATAALAASGTVNTSGLPLTVRS
                     GPGTGYSAVGSLADGTGVTISCQTIGSTVTGTYGTSAIWNKIGTGRYIADAYTYTGSD
                     GFVAPLCTDGSTTHIGNDYPYSGATGGVDPWNFYKGQCTSFAAWRVTRTLGLPFHNYY
                     KGVHWGNAINWDNAARSAGISVTSTPRVGDIAVRNSGTWGHVAYVEKVNADGSFVVED
                     YNHVRAYTYGYWTATRGTGSNQFDQFIHFK"
     gene            18766..19596
                     /locus_tag="PKU75_04845"
     CDS             18766..19596
                     /locus_tag="PKU75_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPF80237.1"
                     /translation="MTTRRALLTATLFAAPTSLLLSEPARAALPTVDMEALLKAAQWD
                     PVKLDTGITAGAGASVLQVEKALVAKGLLAASYADGHYGSRTVAAYKAWQESLGYSGI
                     GANGIPGPASLAALATNRFTVVRPLAPGAKVAFRGVTINSRTRAMLLAAEGKLGFTFV
                     MEQGSYNPGGDVTSAGTHDGGGVVDLDVVALTSSQRTAAATALRQAGFAAWVRSPAMG
                     NWDWHIHAVAISDTDLAPQAQKQVGAYYEGRNGLANNAVDDGPKVTKVTWEEYQRSLA
                     "
     gene            complement(19641..20360)
                     /locus_tag="PKU75_04850"
     CDS             complement(19641..20360)
                     /locus_tag="PKU75_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80238.1"
                     /translation="MTTQPLPHSTPQDGQDPVERYQYLLRTATPDNIEKAHEQAFASM
                     TPAERAEVLEALSRTAETPADDSPAALARSATRLEVAQPGALQQALDGVQSSTGKTLL
                     TSVAGGFLGAALQKGLFGDSGSGDGLIGRLRTGSLGGGLFSGGLFGRLFGRSNTSSTN
                     GNWNDNQNTWNNQGDNQAPPPPPQQDQQGGWGGGHQGGFGPGFSGGGRRGPGGHGGPG
                     GRGGGPGGHGGPGGGPGGGRR"
     gene            complement(20760..21128)
                     /locus_tag="PKU75_04855"
     CDS             complement(20760..21128)
                     /locus_tag="PKU75_04855"
                     /inference="COORDINATES: protein motif:HMM:NF022431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HPF80239.1"
                     /translation="MGRSTTRSFAVLVLTSLVAAGDTDRGAFERVAHWYPHEHWYPHE
                     HWYPHEHWYPHEHDVQAYDAQLGWLHAVPHGADHLGTAAAAGLASPEEVLGILARRIA
                     APAEMWQQLEEARIGVAILE"
     gene            21115..21249
                     /locus_tag="PKU75_04860"
     CDS             21115..21249
                     /locus_tag="PKU75_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80240.1"
                     /translation="MDRPTRQPVEHLGPDPAGLASEPAGLDPVRQLGIRPAVPDQRVR
                     "
BASE COUNT         3068 a         7657 c         7390 g         3213 t
ORIGIN      
        1 ggcgttccgc gatccgtcgc cctggaatgc ggcgacctgg tcgtcgagac gaccaggaaa
       61 cgcgtgcgcc gcggcgaaca cgagatcgat ctcacctccg cgatccactc tgccgtcgcc
      121 tgggcggtcg agccggcctg cgcccgtagc gtgctgagca ggacgaacgt caacgccccg
      181 agcccgatga ggatcaccgc ggcgaacgct ccgagcacca cagccaggac gcgagtgcgg
      241 atcgtgcgca ggacgctcac gtccggcaca ctacgccccg cccgggggcg tgcccggtcc
      301 tcgcgctcag cgccagaacg gctcgagcgc catcacgccg gcccaggtcg tcacgcactc
      361 ggcccggccg tgcacgggcc cgaggacgtc ggtgggcagt ccgtgggcgc gcagcatgtg
      421 cagggcggtg gcgtgatcgt tggcctcgcc gaaggcgtcc tgcatcagac ccagcgccgt
      481 cgagaactcg ttcggcagcg aaccaggggc atcggcatac agatcggcga agaactcaca
      541 cccgtacctc agcttcttgc cctcgatgcg caccttgtgg agttcgtgcg gttgcagggt
      601 gagcaggtcg ggtccggctg cggcgacgcg ggcgtagcgc ttctgcagcg ccgcgtcggt
      661 gaggtcccgg gccggcgcat cgcgcaggcc ggacgcgaag tccagccact gcgggtcggc
      721 cagccaggcc cgcagatcgg ccttgacctc cagccactgc ggcgaccgca ggagcgggac
      781 ggccacggca taggtccggc gccgccgccc ccgcagggtg cgtcgggtgc gatagggcca
      841 gccctcgtcc tcggcctgct tgagcagcac gtcggcgtcc cgagcgggtc cgagagggag
      901 cacgatctcg cgcatccgca ccttgagttc catcgactcc tcgtcgtgct cgaagagtgg
      961 ccggaacaac gacatcgcgg cccgggcccg gcgccacgag acccgcgtct ggtgcaggtt
     1021 gtcgcggtgc cggccctcga ggaagagcgg ctcgttggcg tccaggtgtg ccagacaggc
     1081 ctccaacgac gcgcggaaag cgtcgccgac cgtggcgtcc ggcggcatga caaccggttg
     1141 ggacttgacc gccggcgact ccgtcgcggc tgttgcgggg gtggccggcg cggtcatgtg
     1201 ccggctcagt tcacggagat gtgcacgtgg tccatgtggt tggccgtggg tgacccgcgg
     1261 tcctccatcg cgcgccagcc cccggacccg gcaagccaga tgcgttgctg ccaaatgaca
     1321 tacgagatgc cgaggctgcc catgttcgcg atcgcgtaac tggcgacggc atcgccctgg
     1381 ccggaactgg agatcatcac gtcgaccgcg cgcccggtgc cgtggtcgcc ggggtcgccc
     1441 ggacggacac cgccgatgtt cgtgatgccg aaggtcgagc ggatggccga gtagaccgcc
     1501 gctgccgcgg gagtcagacc catcgccgct ccggcgcccg cgtaggtgga cgaactgcca
     1561 cccgacgaac tgccccctga cgacgaattc gacgaactcg acgaaccgga cgaagaggag
     1621 gaggacgtgg acgactgcgt gcgcgcggtc gtgccggacc cggtcgccgg cgcctgtgcc
     1681 gcgacgggct cgggggccgg cttgggcttc ggcttggcga ccgccttgac gccgagcgca
     1741 ccgtatgccg cgccggaact cagtgacacc ggtgcccgca ggtcggtggt ggcggcgagg
     1801 tcggcgcggg tggtcgagcg ggaggcccgc tgatccgcgg cgcggccggc caccgcggcg
     1861 tcgctgcggg tgacgtctgc gacggcaagg cggcccctgg ggcgggagcg gcggtgcgcg
     1921 aggcgtatgc ggcggcgccg gcgccggcga cggcgagggc ggccgcggcg gtcagggcca
     1981 gcgcgccggg agcgcggcgc gtgcgggcgt gggcgccaga atgggacaac acgatgacgt
     2041 cgggcctttc cggggattcc gtccacggta ccgagccgca ccggcaaatg tcacatcgag
     2101 ataacggtgg tggcgcgggt tgagaggatg cggcgatggg attcacccgc gcccaacttc
     2161 agggtttccg cgatcacgag gtgcccgacc tcatcgggcc gggcctggtg ctgctcttcg
     2221 tcggcatcaa ccccggcctg tggaccgcgg ccacccagac tcacttcgcc cgccccggca
     2281 accgcttcta tcccgccctg ctgcgcgccg gcatcatcga gcggagcatc gaccctaccg
     2341 ccgggatgac cgacgcggac cgcgcctacc tgctgcagcg ggggatcggc attaccaaca
     2401 tcgtccgccg cgccaccgcg cgagccgacg aactgagcgc gcaggagtat gccgccggcg
     2461 ggcgcaccct ccgcgcgacc gttcgcgaac accggccgcg ggtcgtcgcg gtggccggga
     2521 tcacggcata ccgtgccgcc ttcggcgaac gccgggcccg ccccggccgg caacccggcg
     2581 acctcgtggg cgcgcaattg tgggtggttc ccaaccccag cggcctgaac gcccacgaga
     2641 caatcgattc cctcgcgcag gcctatgccg cgccggcccg cgccgccggc ctcctcccgc
     2701 ccacctgaac gccggtttcg ggcgctcacc ggccggccct tattgcgaac gaattgcagt
     2761 agcgtggagc catgcacgtg cccgacggct tcctcgacgc aaccacctcc ctggcgacgg
     2821 ccgcgacagc cgtcgcggga gtcggcctgt ccctgcgggg cgcgcggcgc gagttgagcg
     2881 acttcgccgc ccccctcgcc gggctcgtcg ccgtcttcgt cttcgccgcc cagatgctca
     2941 acttcccggt cggcgccggc acctccgggc atttgctcgg cggcaccctg gcggccgtcc
     3001 tcgtcgggcc ggcgaccgcc accttgtgcc tgaccgtcgt cctgctgatc caagcgctgg
     3061 cgttcgccga cggcgggctg agcgcgctcg gcaccaatgt cacgctcatg gcgctggtcg
     3121 gggtgtgggc cggctatgcc gtcttcctcc tcgcccgccg ggtgctgccg cgccgggtcg
     3181 cgagcgtccc ggtggcggcc gcggtcgggg cgttcgtctc cgtgccggcg gcggccctca
     3241 cgttcaccgg actgtatgcc gtcggcggcg ccgccccgat cgacctcagt tcgctcgcca
     3301 ccgcgatgct cggctggcac tccctcatcg ggctcggcga ggccgccatc accttcctcg
     3361 ccgtcggggc catcacggcc acccgtcccg acctggtcca cggggcccgg gcgctcgcgg
     3421 gagtccagga cctcgagatc cgcgagggcg tgcgcgcatg aaccgggacg tcagcacgcg
     3481 caagcgcatc agcacccgca cgctcctgct gtgggggctg gccgtcacgc tcgtcctggc
     3541 aggcgtcgtc agcttctacg cctccggtca ccccgacggc ctggaattcg tcgctgagaa
     3601 gctcggcttc gacagcacgg ccgccggcca cgccggcggc gactcgcccg tcgccgacta
     3661 cggcgtccgc ggcgtcgaga actcccgcct gtccgggggg ctggcgggcc tgatcggagt
     3721 cctcctcgtg gggatcgtga tgaccgggct ggtggcgctg ctgcgccgcc gcacgcccgc
     3781 gaaacgcgac tgacgtgggc gcggggcacg gccactacct gcactaccac gggcactcgc
     3841 cggtgcaccg gctgccggcg cacaccaagc tcgtcgcatt gctggtcttc gtcggcgcgg
     3901 tggtggccac cccgcggacg gcggtctggg ccttcgccgg gtatgccgcg ctgctcctcc
     3961 tcgcgatggc ccgctcgggc gtgcccgcgg cctacctcgc gaagcgcatg gtcgtcgagg
     4021 tgcccttcct cgtcttcgcc ctgctgatgc cgttcgtggc caccgggccg cgggtggagg
     4081 tgggtccgct gcgcctgtcc gaggccggcc tgtggggggc ctgggggctg ctcgccaagg
     4141 gcacgctggg tgtcggcgcg tcggtgctgc tggggtcgag caccgagccg cgcgacctgg
     4201 tccgcgcgct cgatcggctg cggctgccgc agccgctggt gcagatcgcc tcgttcatgg
     4261 tgcgctatct ggaagtcgtt ctcggagaac tcgatcggat gcgggtcgcg cgcgcatccc
     4321 gagggttcac cgcgcgctcc gtcaggcact ggccggtgct ggccgcgact atcggtgccc
     4381 tcttcatccg ctcctacgag cggggggagc gggtgcatct ggccatgctg tcccgcgggt
     4441 atgccggccg gttgcccttc gccgccgagc tcaccgccac gcgggtccag tgggtccgcg
     4501 cgctcgccct gccgctgatc gcggcggccg ggtgcgtggc ggcgtggatg atggcctcgt
     4561 gatcgcggca tgagcacccc cgtcctcgac ctccgcggcg tcgccttcgc ctatcccgac
     4621 gggcaccagg ccctcttcgg cgtcgacctg cacgtccacc ccggcgaacg cgtcgccctc
     4681 ctcggtccca acggcgccgg caagacgacc ctcgtgctgc atctcaacgg catactcacc
     4741 gcgggcgcgg gctcggtgac gatctccggg ctgccggtga ccaaggacaa cctgctggag
     4801 atccggcgcc gcgtcggcat cgtcttccag gaccccgacg accagctgtt catgccgacg
     4861 gtccgcgagg acgtcgcctt cggcccggcc aacctcgggc tcgcgcgcga ggcgatcgac
     4921 gaacgcgtcg aatcggcgct gcggtcggtg ggcatgctcg acttcgccga ccggcccccg
     4981 caccacctct ccttcgggca gcggcgccgg gtcgcggtgg cgacggtgct cgcgatggag
     5041 ccggagatcc tcgtcctgga cgaaccctcc tccaacctcg acccggcgtc ccggcgcgaa
     5101 ctggccgaga tcatcacggc gctcggcgtg acggtcctga tggtcaccca cgacctgccc
     5161 tacgcgctgc agttgtgcga gcgctcggtc gtcctgtccg acggggtcat cgccgccgac
     5221 ggggccacag ccgacctgct gaccgacgcc gagctgctcg cgcgtcaccg gctggagttg
     5281 ccctacggct tcgacccgag cgtcctggtt cgatccgagc gttgacggag ctcaccctcg
     5341 aggctgagcg acgtcatacc ggggtctgac gatattcatc ccgggtcgag acccagggtt
     5401 gaggacgcgg cactgcgcgc cgcgaaatcc tttgaggcat gaacacattc gccgccatcg
     5461 ccacctacct cctcgccgcc gccggcgtgg ccctgatgct ggcgctcgcc acggtcccgc
     5521 tcgtcctcga ccgcgacgcg gagcgttcgg acctcgcccc ggccgtcccg ctgcgccccc
     5581 gcccggccgc cgccccgacg gccaccccgc gcgccgcctg accggtctac ttgtcgtagg
     5641 ccgtcgcgtg cttgggctga tagatcagga tcgcgtcggc gccgggcacc tcgacctcga
     5701 taccgatgcc gaaccccatg tcggacgggc cggtgaggat gttcgcgccg cgcgcggtga
     5761 tctcctgcgc cgtggcgccg acatcggtgg tcatcaggct cacctggtga tggcgcgggg
     5821 cgctgaacga ctcgccctcg aactcgccac cggtcgggtg gacgccgatc tcgcacggac
     5881 cagcggagaa gatgagccag tcggagcctt cgttgtcgtc gtcggtgacg aagggcagct
     5941 gcagcacatc cttgaagaac gcgcgcgtcg ccgacgcgtc gtcgctgtag atgagtgcgt
     6001 gaacggcatt gatcatgttt ccgaccctac ttgagagccg agctggggga cgacccgttc
     6061 ggcgaccagg tcgaggtggt cgaggtcgct gaggtcgagc acctgcaggt agacccgttg
     6121 caccccggcc tcggcgtagg tgcccagctt gtccacgcac tgcgccgggc taccggcgac
     6181 accgttctcc gtgaggtcct cgatggaccg tccgatggcg gtggcccgtc cgcacagctc
     6241 gtcgtcggtg cggccgcacg cgaggacgag cgccgccgag cgcacgatct cgccggggtc
     6301 acggcccgcc tgcgcgcagg cgtcggcgac gcggtcgtac atctgcgtga cctcggcgac
     6361 cggcgagaag gggacattga actcgtcggc gaagcgggcc gcgagatgag gtgtcttgtg
     6421 aacgcccttg ccgcccagga tgatcggggg gccctgggga gtggcgggct tgggcagcgc
     6481 cggggcatcg gagagcgaat agtgtttgcc ggcaaaggaa tagggctcgt cgatggccgt
     6541 gccccacagg ccggtgatga tctcgagctg ctcctcgagc cgctcgaagc gttcggcgac
     6601 cggggggaag gggatcccgt aggccgcgtg ctcgtcctcg aaccagcccg cgccgagtcc
     6661 gaagtccact ctgccaccgg acatccggtc cacctgcgcg accgagatgg cgagcaggcc
     6721 ggggtgccgg aaggtcgctg cggacacgag tgtgccgagc cggatcgtcg tcgtctcccg
     6781 cgccaacccg gcgagtgtga cccacgaatc cgtcggcccc ggcagcccgt ccgaaccacc
     6841 catggcgagg tagtgatcgg accggaagaa cgcggcatac ccgcaatcct cggcacgtcg
     6901 cgcgacggcc agctggtcgt cgtaggaggc cccttgctga ggctcgacga agatccgcag
     6961 ttccacggcc gtcatcctgg caggcccgcc cagtcaggga tatgacccgc ctgccgcccg
     7021 ggcacgaatt catctgttgt ccatattccg gaggggccca cagcatcgtg gcctacggtc
     7081 gcggcatgga cgggggagca gcgctgcggg agcggctgga gccgctctcg gggcccgtcc
     7141 cgctcgaccg ggccggcctg gtgatcgccg cggtgctggc gggcgtgatc atcggctggc
     7201 cgccgctgtg gcgccgggtg cgtctcgccg tgacgctggt gcacgaggcc gggcatgcgc
     7261 tggtgggagt gctgtgcggg cgccgcttca ccggcttcgt cgtctcgggt gatgcctcgg
     7321 gccacgccgt gaccgtcggg cccgtgcgcg gcctcggccg gatcgccacc acgtgggcgg
     7381 gctatcccgc accggcgctc gccggctgcg gcctggtctg ggcggccgcg cacgggtatg
     7441 ccgcgcccct cctcgccctg ttgctgctcg gggaggtggt gctgctgccg cgcatccggt
     7501 ccgtgatgac cggtgtggtc ctgatcgtcg ccctggcggc gacggcgtcc gcctggtggt
     7561 ggcgcgacga caccgtccag gtccacgcct tgacgatcct cgggctggtg ctgctcgccg
     7621 gcgcctggcg tggtttggcg gcggtatggc agcgcggtcg cgccggcgac gaccacgccg
     7681 tcctgcgccg cctcaccggg gtcccgggcg tcgtatggct gctcagctgg gctctcgtgt
     7741 gcgccgctgc cacgctcgtc gccgccggag aactctgatc cgactacgca cgtgggtggg
     7801 agctcgatat cgcgcacatc ggcccggtag aaggcttcga cggcatcggg attccggccg
     7861 agagtccgca ccggctcggt cgtcagcacc gtcatacctc cttgtcccta cccccttgtc
     7921 gacgcagacg gccggacgtt acatcgtcgg agcaccggag ccgtcggcgc cgtccggcga
     7981 cgccgccacg acggcgcact gggccgggtc gagccggacg gcgatggccg tctcgggcac
     8041 ctgcgcgggg gcccgcacca cggcggtgcc gtcgccgggg atccggaccc ggatcgcggc
     8101 atacccgcga tggtcgacct gctcgacgat ctcgagccgc cgcccggccg ggtcggtcac
     8161 caggccaccg gggccgaccg cgacgagctt gtcccccaac ggatccgggt agccgagtcc
     8221 ggcggccacc ccggccggca acagcgggct gtagccgagg aagtgggcca cgtccgcgcg
     8281 tgccgggttg cgccagacct cacccggcga gccgatctgc gcgatgcgtc cgtcgatgag
     8341 cacggccacc cggtcggcga cggcgaaggc ttcgtcctgg tcgtgcgtga catagaccgc
     8401 cgtcgtctcg gtcgccttca aggttgtgcg cagcatgccg acgaggcgct cgcgcagggc
     8461 acgatcgagg gccgacagcg gttcgtcgag aagcagcagc cggggccggg gagcgagcga
     8521 gcgggccagg gcgacccgtt gcgcctgtcc acccgagagg gtcgtgatcg cccggtgctc
     8581 gtaaccggcg agcccgacca gatggagcaa ctgggtcacc cgggcctgac gttcggcgcg
     8641 cggcaggccg gcgatgccgt aggcgacatt gccgccgaca tcgcggtggg ggaagagttg
     8701 cccgtcctga aacatcacgc cgaagccgcg ctggtgcacc ggggtgcggc tcagatccag
     8761 tccgtcccac gcgacactgc cgccggcaat aggttcgagg ccggcgatcc cgcgcagcag
     8821 ggaggacttg ccggacccgg aggcgccgag cagggcgatg atctcgcccg gtgcgacgtc
     8881 gaggtcgatg tggtcgaccg cggtcaccgg cccgaagcga acgacgatgt cgcgcaccga
     8941 caggccggcc gcgctcacag ttcgctcgct tccgcgggcc ggagccgttc ggccagcagc
     9001 atgaccaccg cggtgagtgt ggtcagcacc accgaagcgg ccagtgccgc accgaaattc
     9061 gcctcgcccg gtctgccgat gagacggaag atcaccacgg gcagtgtggg gcggtcgagc
     9121 cgggcgagga atgacgtggc gccgaattca ccgagcgagg tggccatcgc gaagcccgcc
     9181 gcgagaccga ggccacgcac caggaagggt ccgtccacgg tgcgcagcac tcggccggga
     9241 ccggccccga gcgtcgcggc ggcttcccgc tggcgcggat cgatggcccg cagcacgggc
     9301 aggatcgagc gcaccacgag cggcagcgcc acgatcgcct gggcgacggg gatgagcagc
     9361 atcgaggacc gcaggtcaag gggcggccgg ttcaaggtga tcaggaagcc gaaacccacg
     9421 gtcacggcgg acactccgag cggcaacatg aacgcggcgt ccagggcgcg taaacccttg
     9481 cgcgccaacg gatcacgcgg ccgacgactg acgacgaggc tgaccagggt gccgagcacc
     9541 agcgcgatgg ccgtcgcctc gagggcgacg acgagggagt tgcggatcgc cgtccacacc
     9601 ggcaccggga gcgaccggcc cacggtcgtg ccgagcgctc gatagtgatc gagggtccag
     9661 ccctcacgat cacgcaacga acgcaggagc atcgtcgcca tgggcagcgc gaggacaccg
     9721 agcaccgtcg cgccggtgac cagtgtggcg gcccagtcgc ggcgccacga ccaggtgtgc
     9781 tcgccactcg cgtccccccg caatcgcagc gcgcgctcct gccgggcact cgtccacgac
     9841 gtcgccagca gcgcgagcgt cacgacgacc agctgagtga tcgacagcgc cgccgcagcg
     9901 ggcagatcga gcaattgcgt tgtctgatac cagatctcgg tctcgatggt gctgtagcgg
     9961 acgccgccga ggaccatgac gatcccgaac gcggacgcgc agaagaggaa gaccagcgtc
    10021 gtcgcggagg cgatcgccgg caggagcgcc ggcagggtca ccgtgcggaa agcccgggac
    10081 gggctcgcct ccagcgcccg ggcagcctcg accgcccgcg gatcgaggcg tgaccacaat
    10141 ccccccacgg tccgcaccac caccgagtag ttgaagaaga cgagcgcggc gacgatcgcc
    10201 ggcagcgact cctcccaccc caagaatccc aacgggccgt tcgtcccgag cagcgacagg
    10261 aaggccacgc ccacgacgac actcggcagc acgaagggaa ccgtcacgag cgcccgcagc
    10321 agcccacggc cggggaagcg aacggcatac agcaggtagg cccccggcac tcccagcgcg
    10381 accgacacca cggtgccgag cacggccagg gtcagtgtgg tgcgcaggat ccgccacgtg
    10441 cgcgcggacg tcagcacctc ggccaccgcg ccgagcgtcg atccgtcctc cgaggcgaac
    10501 ccgcgcccga ccagcgtcgc gacgggccac gcgaagaaga tcgcgaggaa ggccaacggg
    10561 atcgcggccg cgaggagcca gccgcaccgc catacccacg acggtccgcc ccgggcgcgc
    10621 cgatcggccc gggcgggtgc ggtcgaggtc gtcacgtcag cggtcagccc aagacctcgg
    10681 cggtccagtc cttgatccag gtctcacggt tcgcgccgat cgtggctgcg tcgaccgcga
    10741 tgggcttcgt ggcgagcggg gcgaacttga cccactcggc cggcagctcg accgaggagt
    10801 ccacgggata catgtacatc tgaccgggaa tgtccgcctg gacacccgcc gtgagcaggt
    10861 aatcgatgaa cttctgtgcg ccccactcgt tctggctgcc cttgagaatg cctgcgtact
    10921 cgacctggcg gaagcacgtg tcgagcagtg ccaccgtgcg cgactccttc gcaccctcgt
    10981 ccacctcgaa cgcgggggac gtcgcgtagc tgacgaccag ggggcgcggg cccttgccgg
    11041 acgaaccgga gaagtccccg gtgtaggcct cggtccagcc cttggccacc ttgacaccgt
    11101 tgtccttcaa cgacttccag tagtcgaggt agccgttctc gcccttggcg ccgacggtgg
    11161 cggcgaggaa ggccaggccg ggagaggagg aagcgggatt ctcgacgacg aggagatcct
    11221 ggtatgccgg cttcgccagg tcgtcgaggg tggccgggac gtccagcttc ttgtcggcga
    11281 accagccctt gtcggcgttg acgcacacgt cgccgtagtc gatcggggtg agcatccccg
    11341 agtcgtccgc cagcatcgcc ttggcggact cgggcagggc ggcgcccggg tacgcactga
    11401 ggaccccggc ggtctcgacc cggctcgcga aggtgttgtc caccccgaag acgacatcgc
    11461 cgaggggtgc gtccttggtg agcaccagct ggttgaccac cgtgcccgcg tcgcccggcg
    11521 ccacataggt gaccttgatc ccggtgtcgg actcgaactg cttcttgacc tcgtcggaga
    11581 ggttgaacga gtcatgggtg acgacggtga ggctgtccga gcgggtcagt tccgcgctcg
    11641 cgctctccga cgccgactcc gacgcgctag ctgacccgct ctgggaggcg gcggaactgc
    11701 tgctcgaggt gcccgcgctc ccgccgtcag aacacgcggc cagcaacgcg acggcgacgg
    11761 ctccggcgca ggcggcccgc ggcatacggg tgcgtcgtga cgtgatcagt gtggtcatga
    11821 tgactcctcc atcaatctgg agggctcggc atgtgccgag gagaatcccg actccctacg
    11881 ccggtgcgaa ccggatcagg tccgagggtc tgcgctcacg cgcactctca gcgcccggtg
    11941 gcgctcccct gtctgtgggg tgaacgctag caccctcggc tgctgttccc gcaggtaggc
    12001 gggaacggcc ggggcaggcg agcggatcag tcgagatact gcgcccggat ctgctcgcgc
    12061 cggatctccc aattcgcgat gatccggtcc atctcctcag tcacgaacgc atagaagtcc
    12121 gccgaatagc ggagccggcg ccctgccgcc gtctcgaggc ccacagcctc ggctccgcgc
    12181 tggagctgat cgaccagata gccgatgaca gcggccttct gcagcgtcac gtcgtgccag
    12241 gtatcggtat gcaggacata gacatcgcgc cgcgtgccgc gttcgcgttc cttggtcacg
    12301 aagccgtagg cctgcaggaa tgtgatggcc cccgacactg cggcccggct gacgtgcagc
    12361 gcctcgcaga tctccgccga cgtcatccgg ccgtcgtcgt cggcgagcag gaccgcgatc
    12421 accctggcgg gcagccgttg catccccgca gcggtcagcg tggcaccgaa ggtctcgacg
    12481 aaggcggtca cccgtgactc gtcgcgaccg ccgccgctcg cctcgttccc gctgctcgcc
    12541 tccgtcatgt caccgcctcc gtcactgccg cccggccacg taatgacagc agattagcaa
    12601 cattcacaaa ttcctgacac cagtggcact atggaggggt ggcacatgtc atcgatatcg
    12661 ccggcctcgt caagaccttc ggggcgaccc gggcgctgga cggcctcgac ctgcaggtcg
    12721 aatccggtca ggtgcatggc ttcctcggac ccaatggttc gggcaagtcc acgacgatcc
    12781 gggtcctgct cggtctgctc cgcccggaca gcgggaccgc ccgcctgctc gggggcgacc
    12841 cgtgggccga cgcgccggcg ctgcaccgcc gtctcgcgta cgtcccgggc gacgtctccc
    12901 tctggcccaa tctcaccggc ggcgagatca tcgacctgct cggccgggcc cgcggcggac
    12961 tggaccaggc caagcgcgcc gatctgatcg aacgcttcga cctcgacccg acgaagaagg
    13021 gccgctccta ttccaagggc aaccggcaga aggtcgccct cgtggccgcc ctcgcctccg
    13081 acgtcgaact gctcctgctc gacgagccca cgtcgggcct ggacccactc atggagcgcg
    13141 tcttcaccga catcgtccgc gagcggcgcg atcagggcgt cagtgcactc ctgtcgagcc
    13201 acatcctctc cgaggtcgag aagctctgcg atcacgtgag catcatccgc gccggacgca
    13261 tcgtcgagtc gggcacgctc tcccagctgc gacatctgac ccgcacccat atctcggccg
    13321 aattggcttc gccaccaacg gatctgacgg ccttccccgg cgcccacgat gtcgccgtcg
    13381 acggggaccg gttctcctgc ctcgtcgaca gcgcgcaggt cggcgacctc ctcgcccggc
    13441 tcacggcgca cggggtgcgc tccctcaccg ccactccgcc gaccctcgag gaactcttcc
    13501 tcgagcacta cgacctgccg gaggaggcgt cgtcgcgatg acgaccgcca ccctccccgc
    13561 ccccgccacc gtgcgcgacg cgggcccggc cgccggcacc cgggtggtgc tgcgccgtat
    13621 cctgcgagcc gaccggtggt tctggctggt ctggctcgtc atgttgtggg tcacgatccc
    13681 ggcgaccgcg agcgcctatc gcgagacgat caccgacgac gcggccggtc gcgaggtcgc
    13741 cgcgggcctg gccaccaatc cgacgatgcg ggcgatgctc ggcccgccct acgacctgtt
    13801 gcacgtcggc gggttcacgt cctggcgtgt cggcggcttc gtcgcgatga tggccgcggt
    13861 catggcgggg ctcggcgtca tccgcgccac tcgcgcggac gaggaggcgg gccgcaccga
    13921 actgttgcgc gccgggtcga tcgggcggca cgcgcccctg cttgccgccc tgctcgtcgc
    13981 ctccggggta tgcggtctgc tcgccctcct cgtcatcgtc tcgatgatcg gcctgggcac
    14041 cccggcatcc ggcgccgtcg cgctggggct gggcatcggc gtgaccggtg tcgccttcgc
    14101 cggcgtgggc gccgtcgccg cccaactcac cgcgtcggcc cggaccgccc ggggcatcgc
    14161 gatgggatcg gtcggcgcgg catacctcct gcgcgccgtc gccgacggga cctcggagga
    14221 cgccggtatg cggtggctcg gctggctctc gccgctcgag tggtcaccgt acgtgcggcc
    14281 gtatgccggg gagcgctggt gggtgctcgc cctcccgatc gcattggcgg ccgggctgat
    14341 tgggttcgcc ttcgcactgg aggcccgccg tgatcacggc gcgggcctgc gccccgcccg
    14401 ccccggcccg gagcacgccg caccggccct gggatcggcg agcgctctcg cgcggcggct
    14461 cgatcggggc acggcgatct ggacactcgt cgggatgggc cttttcgcgc tcgtcatggg
    14521 ctcgatgtcg aatgtcttcg actcgatcgc cgaagatccc acgctggccg atcgcttccg
    14581 ccggatgggt gccggcgcga ccgacctgac cgacgccttc tatgtggcga tgatcggcat
    14641 cctcgtcgtt ctcttcgccc tgatcggcat ctccctcgtg gagcgactcc ggcgcgagga
    14701 ggagtccggg cgcggtgagc tgctgctcgc cggcgcgacg tctcgatccc gggtgttgag
    14761 tgccttcgtc ctgccggcgc tgatcgtgcc gatcggggtg ctgatcctca gtggtctctt
    14821 gatggcgctg ccggcggcgc tgcgtggcga cccgcagcaa ctcgcacgga tcggcggcgg
    14881 cgccgcggcc caggcacccg ggatcgcact catcgtcggc ctcgcggttg ccgtgcacgg
    14941 gttggcgcca cggctgcggc tactgccctg gctcgtcacc ggctggtcgc tcgtcgtcaa
    15001 ctggatcggc gcggtgctgg gcttccccca gcgagtcctc gacgccaccc cgttcgccta
    15061 tctgccacga cctccggccg agccaatgga ctgggtcccc gtggtcgtcg aatcgctcct
    15121 ggctctggga ttgctcgcgg ccggctggtg gggctacgcc cgccgcgacg tcacctgagc
    15181 gggccgggcc gttccgtagg atcggccgcg tgcctcagaa cgagttcggc caacccgtcg
    15241 gcgagccgct gcccggctgg caggcccggc cacccgtcat acccgtgacc ctgaccggaa
    15301 ggcacgtgcg gttggaaccg ctcacggcgg cacacgccgg aattctctac gcgccgatgg
    15361 ttctcgaatc cgacccgcgg atctggacct actaccccta ctcgccgacg atgacccgtg
    15421 ccgagttcga cgcctgcatc gccgtccagc tgcgcaaccc ggccggcgcg gcgcacgcca
    15481 tcatggcgcc ggacggtgcc ggtctggggt tcgcgagtta cttgcggatc gacccgccga
    15541 acggcagcgt cgaggtcggc gggatcgtct attcctctgc gctgcaacgc accacggccg
    15601 ccacggaagc gatgatcctg atggcccggc atgtcttcca cgacctcggc taccgccgat
    15661 acgagtggaa gtgcgactcg tgcaacgaac cgtccaaggc ggccgcgacc cggctggggt
    15721 tcacctacga agggcggcat cgcaacgcga tggtctacaa gggccgcaac cgcgacaccg
    15781 actggttctc gatcaccggt gcggaatggc cggcgctgtc cgcggcatac gacgcgtggc
    15841 tggcccccgg caacttcgat gccgcaggag cgcaacgccg ctcgctcggt gatctcaccc
    15901 gttcctgacg gggcgcagcg gaaaggcggt gacccggtcc gggtcaccgc cttcgctgcg
    15961 tcggtcgctg tcgaggctcg accggggtgg gatcgaccga tcagtgaccc gtcacccagc
    16021 aggcgatgca ggccccgtcg gacgtggagt tcgggccggc gatgtcggcg tgcgccgccg
    16081 gggcgagacc gaggccgccg agaaggagga aggcgagggt tgcggtgaaa cggcgcatca
    16141 ggtcagtcct ttcgtgtcgt ggcccggctg gcgtccggat ctgggggaag tcaaccggcc
    16201 cggctcgccc gcgccaaggg gtctgggcga cgcacaaact gccatggcag cactttcggt
    16261 agccatcggc ggcggcacct ggatctccag cagctcgccg gcctcggtga actgggcgag
    16321 tattccccgc aacaggcggg tcgccgcacg acgaacggtg tcgggggtgc ccacggcgaa
    16381 gatcagtccg tcgatgaacc cgtccgccgg atccggctgg atgctcacgt gttgcagcac
    16441 gccgtccgct cccgcactac cactgtcatt gaatgccgcg atcgcggagc ggatctctag
    16501 gaggacgacc tcgctctcgt cggcaagtcg caaattctct ggatatttcc aggcaaggcg
    16561 aaatgggtaa atatccatga tcgacccttc cgtcgttaaa gacatccgga tcatcgtcgc
    16621 cacaattggt cgtgtagtgt gcgagatatg cgggggagaa cctgacatga caggaagtga
    16681 cggctccggg gcccggccgc ccgtggatgg cctcgcggtt ctcgggctcg agccggtcac
    16741 cttggacgtc tatcgccagt tgctgctgcg tcccgaggcc gacctcacga ccctcgccga
    16801 ggcctgcggt gtcggcgagc cgcaggcacg cgacgctctc gatgcccttg ccgccaccgc
    16861 cctgctgcgt cccagccaga gccacgccgg cacctggcgg cccatctctc ccgaagtggc
    16921 cctgcgccgg ctcgtcgccg agctcgagga agcgcacgcc cagcaggccg tccggctcgc
    16981 ccgggttcgc gacgacgtgc tcgccttcgt caccgactat cgcgaagaac gcgaagcccg
    17041 gctcgcccgc accgtcgaac gactcgacgg tcgtgacgcc gtggtgtcga tgctcgaaga
    17101 actcagcgcc gccagcacga ccgagatcct gtcgatgtcg acgtcccgac ccgcgcccga
    17161 ggccgtcgag caggcacgcg actcggacac ggcgacgctg gcgcgcgggg tgcgctcccg
    17221 cacgatctat ctcgagggtg tcgtcgacaa ggcattccgg cagtcgctct tcagcttcgt
    17281 ctccgagggg gccgaagtgc gcctggcccc gactctgccg gtgcgcatgc tcatcttcga
    17341 tcgtgcggtc gccgtcatcg cgaccgatcc ggacgacatc ggcgccggcg ccgtcgtgct
    17401 gcgcggaggc ggcgttctca tcgccctcgt tgccctcttc gagatgcaat gggaggccgc
    17461 ccgcgccccc ttcgaggtcc cgacgcccga gaacgatgtc gaggtgttgt ccccgatgga
    17521 cacgcaagtg ctgagactgc tcgcccgggg tctcaaggac gaggccgtcg cgcaccacct
    17581 cggtgtctcc gtccgcacgg cccgtcgttt catcgccgat ctgcacgagc gggccggtgc
    17641 cggcagtcgc ttcgaactcg gtgtccgcgc caaggaactc ggctggctct gacctgatcc
    17701 gaacaggtcg cggccgcaca aactgccatg acagtttgtg ccgcacaaaa actcttccct
    17761 tgccgtggct gggctgatga agtctctttc ggcagccagg agagatcctg acagaaaacg
    17821 ccgaagcccc ttctccgcct tcatttcatt tcgtcattcc tgaaaggaat tcgccatgcg
    17881 cacgtccgtt cgtcgtcccg ccgccacctc gatcgccgtc ctcgcagccg tctcgctcag
    17941 cgcgaccgca gccctggccg cgagcggcac cgtcaacacc tctggtctgc cgctgaccgt
    18001 ccgcagcggc ccgggaaccg gctactccgc cgtcggcagc ctggccgacg ggacgggcgt
    18061 gaccatcagc tgccagacga tcggcagcac ggtcaccggc acctacggca cctcggcgat
    18121 ctggaacaag atcggcaccg gtcgctatat cgccgacgcc tacacctaca ccggttccga
    18181 cggcttcgtc gcccccctct gcaccgacgg ctcgaccacc cacatcggca acgactatcc
    18241 ctacagcgga gccaccggcg gggtcgaccc gtggaacttc tacaagggcc agtgcacaag
    18301 cttcgccgcc tggcgcgtga ccaggaccct cgggctgccc ttccacaact actacaaggg
    18361 cgtccactgg ggcaacgcga tcaactggga caacgcggca cgctcggccg gcatctcggt
    18421 cacgtccacc ccacgcgtcg gtgacatcgc cgtgcgcaac tccggcacct ggggccacgt
    18481 ggcctacgtc gagaaggtga acgccgacgg ctccttcgtc gtcgaggact ataaccacgt
    18541 ccgcgcgtac acctacggct actggaccgc gacccgcggc acgggcagca accagttcga
    18601 ccagttcatc cacttcaagt gaccaccccg cgcagctgac tggcgcacct gccggggtga
    18661 cgccgccgcc cggtcccctc cgccgggcgg cggcgcttcg cgcccgccac accgcatacc
    18721 cgaccccaca accgtcaacc ggcaattcac gaagagaaca ggaccatgac gacccgacgc
    18781 gccctgctga ccgcgaccct gttcgcggcg cccacgagcc tcctgctgtc cgaacccgcc
    18841 cgcgcggcac tgcccaccgt cgacatggag gcgctgctca aagcggccca gtgggatccg
    18901 gtcaagctgg acaccggcat cactgcaggt gccggcgcga gtgtgctcca ggtcgaaaag
    18961 gccttggtgg ccaagggttt actggccgca agctatgccg atgggcacta cggaagccgc
    19021 accgtcgcgg cctacaaggc gtggcaagaa tccttggggt acagcggaat cggagccaac
    19081 ggcatacccg gcccagcgtc cttggccgcc ctggcgacga atcgcttcac cgtcgtcagg
    19141 ccactggcac cgggcgcgaa ggttgccttc cgcggggtga cgatcaactc gcgcacccgg
    19201 gccatgctcc ttgccgccga gggcaaactg ggcttcacct tcgtcatgga gcagggctcc
    19261 tacaaccccg gtggggacgt gacatcggcg ggcacgcacg acggcggcgg ggtcgtcgac
    19321 ctcgacgtgg tcgcgctgac gagctcccag cggaccgcgg cggccacggc gctgcgccaa
    19381 gccggctttg ccgcatgggt gcgctcaccg gcaatgggca actgggactg gcacattcac
    19441 gcggtcgcga tcagcgacac ggacctggcg ccgcaggcgc agaagcaggt cggtgcctac
    19501 tacgagggtc gaaatgggtt ggccaacaac gctgtcgacg acgggccgaa ggtcacaaag
    19561 gtcacgtggg aggagtatca acgcagcctg gcctgatcgc ggcccacggg gggttgtggt
    19621 gcgttgcgat ccgtgccgag tcagcggcgc ccgccgccag gtccaccgcc gggaccgcca
    19681 tgtccaccgg ggccgccgcc gcgtccgccc ggcccgccgt gcccgccggg tccgcgccgg
    19741 ccgccgccgg agaagcctgg accgaacccg ccttggtgtc cgccgcccca gccgccctgc
    19801 tgatcctgct gcggaggcgg cggcggggcc tggttgtcac cctggttgtt ccaggtgttc
    19861 tggttgtcgt tccaattgcc gtttgtggat gaggtgttcg agcggccgaa caggcgcccg
    19921 aacagccccc cgctgaacag gccgccaccg agcgagccgg tgcgcagccg gccgatcagg
    19981 ccgtcgccgc tgccgctgtc gccgaacagt cccttctgca gagcggcgcc gaggaagccg
    20041 ccggccacgg aggtgagcag cgtcttgccc gtcgaactct gcacgccgtc gagggcctgc
    20101 tgcagcgcgc cgggctgggc cacctcgaga cgggtcgccg atcgggcgag ggcggcgggc
    20161 gagtcgtcgg ccggggtctc ggcggtgcgc gacagagcct cgagcacctc ggcgcgctcg
    20221 gccggtgtca tcgacgcgaa ggcctgctcg tgggccttct cgatgttgtc cggggtggcg
    20281 gtgcgcagca ggtactggta ccgctcgacc ggatcctggc catcctgagg ggtgctgtgg
    20341 ggcagtggct gagtggtcat cggtggctcc tgggtgatgg tgtctgctgt gctgtcggtt
    20401 tgaagttcat cgcccccgac ggtgacgagc ctgaggtatg gctgtgtgcc tcctgtgagc
    20461 cggggccgtc cgctgcagtg gtggccggcg tcccgtggcc ggggctgaat gaaccatccc
    20521 tcgacgcgcg cgagcacgcg cgcgcacgcc gagcgcaccg cgtcggcgtg cgccacctcg
    20581 accgaatggt cgcccatcat cggctgctcc gcgagcgcga catacaggct cgtgagggtc
    20641 atcgtgacat tccgcagcca cgccggcggc ggcacgacgc ccgcgacatc ggcgagcgcg
    20701 gcctcgacga tgtccagcca gtccgtcgca ccgcccgcgg tcagatccgg ctgggtgagt
    20761 cattcgagga tggccacgcc gatgcgtgcc tcctcgagtt gctgccacat ctcggcgggg
    20821 gcggcgatcc gccgggcgag gatcccgagg acctcctccg gtgacgcgag tcccgccgcg
    20881 gcggccgtgc cgaggtggtc ggcgccgtgg gggacggcgt gaagccagcc gagctgggcg
    20941 tcatacgcct gcacgtcgtg ctcgtgggga taccagtgct cgtggggata ccagtgctcg
    21001 tggggatacc agtgctcgtg gggataccag tgcgcgacgc gctcgaaggc cccccggtcg
    21061 gtgtcgccgg cggcgacaag cgaggtcagg accagcacgg cgaagctgcg cgtcgtggat
    21121 cgccccaccc gccagccggt cgagcatctc ggtccggatc cggccggtct ggcgagcgaa
    21181 ccggccggac tcgatcccgt ccgccagctc ggcatacgcc cagccgtccc ggaccagcgg
    21241 gtccggtgat ccgagtccgg cgagcaggtc gtcgagggaa tgcgtcacct ctgcggcgct
    21301 agcaagcgga tgactggtcg agcggtcc
//
LOCUS       DAOPJG010000032        20852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2701_length_20852_cov_7.922345, whole genome shotgun sequence.
ACCESSION   DAOPJG010000032 DAOPJG010000000
VERSION     DAOPJG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 20852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20852
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2701_length_20852_cov_7.922345"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..660
                     /locus_tag="PKU75_04865"
     CDS             112..660
                     /locus_tag="PKU75_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPF80241.1"
                     /translation="MDTIGLLAEQLDWHWTNQLRPRLAGLTDQEYFWEPAPGAWTVRP
                     RDPAASGPQPGSGAMTIDWAYPEPVPPPVTTIAWRLGHIIVGCLGARNVAHFGGPACS
                     CDTFLYAATADEALAQLDSAYAGWLAGVRGLDDTGLAQPCGPAEGPWADLPMAALVLH
                     INREVIHHGAEIALLRDLWRAR"
     gene            657..1631
                     /locus_tag="PKU75_04870"
     CDS             657..1631
                     /locus_tag="PKU75_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPF80242.1"
                     /translation="MTRIAVVGGGIAGLSLAAALDPDATETTVYESQPERSALGAGLV
                     IWPAALRALTRIGAWDLLRADATPVPPAAIRALSGRILLRTRETGMVIVPRPALLAAL
                     HAVLPDSVQVVAREVTDARALDADIVVGADGVRSVVRGLVAPRVAERRATPYAALRGT
                     RSTVPVEVAGEYWGRGGLFGIVPMGAGAYWFTTHRSGLGPEPLDAGALIGEAQAVFAG
                     AAPVIAETLAAAAPDTLATRLWITPPLPRYRRDRYVVIGDAAHAMTPNLGRGACDAIV
                     DAVTLAGALRTGHVAAWEARRLPVTQAARALSGGIMRMALSGIPSRRR"
     gene            1756..1926
                     /locus_tag="PKU75_04875"
     CDS             1756..1926
                     /locus_tag="PKU75_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80243.1"
                     /translation="MTEDNRATAPRTCRDCGQLLNATPDGSETADDAADLDWTDFCPS
                     PDCPVKLGEAAG"
     gene            2032..3189
                     /locus_tag="PKU75_04880"
     CDS             2032..3189
                     /locus_tag="PKU75_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF80244.1"
                     /translation="MFELSDEELAVVELVRDWVDREVKPVASELEHADRYPEALIEQM
                     KQMGIFGLAIGEPWNEAGVSAACYAAVTEELARGWMSLAGAMGGHSVVAKLISTFGTE
                     QQQEHYLPRMATGEVRATMALTEPGGGSDLQALRTTARAEGDHYVVNGAKTWITNARR
                     SQLIALLCRTDPQADPPHRGISILLVEHGAGLAVSRDLPKLGYKGVESCELAFTDFRA
                     PRSALLGAEEGRGFAQMMRGLEIGRIQVASRALGVGRAALEDALRYAQERQSFGRQIW
                     EHQSVGNYLADMATALTAARQLVQYAARCYDSGRRCDMEAGMAKLFASETAMTIALNA
                     IRIHGGYGYSTEFDVERYFRDAPLMIVGEGTNEIQRNVIVKELVRRNLVAS"
     gene            3186..4043
                     /locus_tag="PKU75_04885"
     CDS             3186..4043
                     /locus_tag="PKU75_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mesaconyl-C4 CoA hydratase"
                     /protein_id="HPF80245.1"
                     /translation="MTAAEPGSPPIVVERNEILLPGPAAALAGLLDVADPGLEAGGRL
                     PMLWHWLYTLDRPPRKDLGTDGHPVRGALPAPPEPGRRRMWAGGRVTTHAPLHAHVPV
                     RRHTRLLSEVTKEGRTGTLTFATVLHEISQDDRVAVREEQDIVYRAAPTAPTADGRPP
                     GDPEPPLPVAADEWAIDVDPVLLFRYSALTYNGHRIHYDRDYARDVEGYPGLVTHGPL
                     QAVCMAEAARRGGIDASVGYHLEYRLLAPLFDHQGFVVRCAPASNGADLSVRDRWGRQ
                     TAAGVLARL"
     gene            complement(4118..4798)
                     /locus_tag="PKU75_04890"
     CDS             complement(4118..4798)
                     /locus_tag="PKU75_04890"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HPF80246.1"
                     /translation="MAAAALGLTGAGAAGARSIAFDEASRTASTSTADAQLAAILSFN
                     REEERMARDLYRLFSNTYNGAAPFSRIVLSEQRHFEAIGRLLTTYRIPDPSAGRPAGS
                     YADPALQKLYTTWKAQGLISRTEAAKVGVTLEKRDIADLKKSIAATTKTDVKNVLSQL
                     LAASQQHLAAFTAAVNGTTMGGGMEPGPMQGGRGANGPLGQGHRAGSGSDTMVCDGTG
                     PRMQRGRP"
     gene            4835..5137
                     /locus_tag="PKU75_04895"
     CDS             4835..5137
                     /locus_tag="PKU75_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF80247.1"
                     /translation="MRAVAPRGRIVVRSIRSGASAMLTVTDDGEGLDSADLERIFERF
                     YRVPGRRSVEGESGHGIGLTISRALARGHGGDLTAASPGAGQGATFTMTIPLRDDA"
     gene            complement(5186..5704)
                     /locus_tag="PKU75_04900"
     CDS             complement(5186..5704)
                     /locus_tag="PKU75_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HPF80248.1"
                     /translation="MSSTPTLGAATAPPRLPPAPVIKSAWRIHRLLYRVSGGRFLWTP
                     ANKRGWGALRLTTTGRRSGADRSVILAYLTHGDGWSVVAMNGWLPGHPAWLLNLRAQP
                     SATIRLKGGPARDVVAREAKGAEREAIWARWLAVDPNLGAWAAARSAPTPVVVLEPSA
                     ASAGAWRDELRG"
     gene            complement(5838..8141)
                     /locus_tag="PKU75_04905"
     CDS             complement(5838..8141)
                     /locus_tag="PKU75_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HPF80249.1"
                     /translation="MRKTPAISAAALALTMVAGATAAGGSTDGETARTGPFARMATYP
                     VYQNVPAGVDPLEETVAEISTVSKDGHTLIYTDAAGKRIGFVDISNASAPNGLGTLSM
                     EQLGDADAEPTSVHVVGGFVLVVVDTSASYVEPSGKLVVVRLSDRKVVRTIALGGQPD
                     SIDVSADERRAIIAIENERDEDVNDGELPQLPAGFLQVVDMPTASPSSWVATKVPLTT
                     ATGAPIPMLAAAGLDTPRDPEPEYVTINSRGVAAVTLQENNGIVLVDLATKKVTKAWS
                     AGIVTRTRVDTLEDGNISLTGTITVPREPDAIAWVDDRYLATANEGDWKGGSRGWTVF
                     DSATKGIAWDSGVSLERLAVKYGLHNEGRAENKGTEPEGLAVATMGGKRYAFVASERS
                     NFVAAYDMTKPMSPVFRQLLPTTNGPEGVLPIPSRGLLAVSSEVDDSDAQVRSSVNLF
                     KLGGPDNRLEFPTIVSAYAGGAPIGWGALSGLSAIPGTTNRLAAVSDSAYATGRIYQI
                     NTAKRPALITSAVDVTDESGVAVGLDLEGISARAAGGYWLGVEGATGAENALVRTTSA
                     GRIQQRVSLPADIAAHVGKWGIEGVSAIGTGADEVVYVALQRPLWADPAGADGPIDGE
                     NVARIGRYSVATGTWSWFGYRLTTTTTDGDWMGLSEITALDANTVAVIERDKLNGPNA
                     RVKRVYAVDVPAKGAPAVNLPILPKRLVVDVLPALRSTRGWTQEKLEGFTVSGGTMVA
                     VTDNDGLDDATGETVFLRLGALPDGAN"
     gene            8420..>9325
                     /locus_tag="PKU75_04910"
     CDS             8420..>9325
                     /locus_tag="PKU75_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HPF80250.1"
                     /translation="MLPPLLLLSLAAPSAIAASDDEDRLPRGAVDTRVETPTLYDDDA
                     GGNASGDDPAIWVHPSDSRRSIVIVTAKEGGLRVYDLRGRQLQKLAATPAPRPDGVPG
                     RFNNVDIARGLMVGGKRTDVAVVSDRYNDQLRFFAINPAGAAATRQLREVTAPGVPFV
                     FQHTRAGVDTEKTAYGLAVWQPTGGPTYVALTQEGTTRIATVRLTISPNGIGYTDSRH
                     LPLPASFPLPNGTTWSVCNEPGTRPQLEGMAVDNARNVLYAAQEDVGLWRIPLPLYSG
                     QPKLIDKVTDFGITDRWDEESEECVP"
     assembly_gap    9326..9425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9426..9861
                     /locus_tag="PKU75_04915"
     CDS             <9426..9861
                     /locus_tag="PKU75_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HPF80251.1"
                     /translation="TDFGITDRWDEESEECVPVDPDQPATYAGHLLTADAEGVAIAHA
                     GAGGQIVVSSQGDDRFAVYNLTGANRPLGSFRIRGIDGADNVNGSDGLDVVTVPVAGY
                     PRGLLVSHDEPDSGPGVDPERDPTNFSYVQWGAVVDALALPR"
     gene            complement(10132..10839)
                     /locus_tag="PKU75_04920"
     CDS             complement(10132..10839)
                     /locus_tag="PKU75_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF80252.1"
                     /translation="MNPTIARLSAQALLGRRRGVVLLVIPALLIGLAVLLRVLTDADG
                     GYDAVLTLGYTLALPLVALLAASAVLGPEIDDGSIVYLLAKPVSRHVIALSKFVVAWL
                     ATLALGALPLVVAGLIMDSGNPRRALAWGAAGAVAGTTYSALFLALAAVTRHAVVAGL
                     LFVLMWEGLLGGLLTGIKWVSIGAWGRQVGYRFSARLDDAFLPLSYAVLAALVVTLGA
                     LWLTGDRLRSFTLRGDE"
     gene            complement(10836..11756)
                     /locus_tag="PKU75_04925"
     CDS             complement(10836..11756)
                     /locus_tag="PKU75_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80253.1"
                     /translation="MSVLTLDAASRWFGNVVAVNGISMTVGPGITGLLGPNGAGKTTL
                     IAMMAGFLAPSAGTVTLDGKPIWRHTDAYRTIGLVPERELSFGYLTGRQFVRANAELH
                     GLPDPGAATERALATVEMTDPADRRIDTYSKGMRQRAKLASALVHDPAVLLLDEPFNG
                     VDPRQRMHLMALLRRMGEEGRTLLFSSHILEEVEQVARHIEVVVSGRHAASGDFGTIR
                     RLMTDRPMQYAVASSDDRRLASALMTEPSVLGVTLRRQGGLDVSVADLGAFAVRLPHL
                     AREGGIRILELTPSDESLESVFAYLVGGPA"
     gene            complement(11753..12655)
                     /locus_tag="PKU75_04930"
     CDS             complement(11753..12655)
                     /locus_tag="PKU75_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF80254.1"
                     /translation="MSQPGGIPANPNSASVIHDIGYRPYAGPRHGEGAIARALVTTGF
                     RNAFGLGRSGRSKILPFVLLAFNLAPALAIAGAIMLANLKEMPIGYARYAATTQVLLG
                     IFVAAQAPVLFSRDQRHGTLALYLARPLPAPAYAIARWLALLGATLVFLILPIVILYA
                     VALLGEFDLTDETRKAAVAVGLALLLTAAITGIAGLIASVSTRRGFGVMATIAFLLLG
                     DGIVAAIQGISSQEGNERIGEIAGLFSPYSLYRGLVTAWADGSSMTPPSSTAMEWLYA
                     AVLLAIALACVAGLVWRYRKLATK"
     gene            complement(12652..13599)
                     /locus_tag="PKU75_04935"
     CDS             complement(12652..13599)
                     /locus_tag="PKU75_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80255.1"
                     /translation="MDQVVVTEGLTKDYGAVLALDGLTVQVAPGVTGLVGANGAGKST
                     LIKILLGLIPPTAGRARVLGHDIATEGPQIRAAVGYLPEHDCLPPDVSASDFAVHMAM
                     MSGLPRVAARERAADVLRHVGLAEERYRPMGGYSTGMKQKAKLAQALVHDPKLVFLDE
                     PTNGLDPAARDEMLGLIQRIGHDFGISIIVTSHLLGELERVSDQVVVLDGGRLLRASR
                     TDDFLDATGALLVEVLGDDAQARMLDALTRAGLTAARRGSMLTVDASHPAGVQDGGAD
                     ADVHDVIRDTAGALGLGLVRLQADHRRIEDVFADTSGPR"
     gene            13981..16254
                     /locus_tag="PKU75_04940"
     CDS             13981..16254
                     /locus_tag="PKU75_04940"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HPF80256.1"
                     /translation="MSIAPKDEMARLLAGERPTVRGHGQLRVDLDDLRVEVAGLGELR
                     QPVTASTAKKLAALGKPAAFGLGTQTVLDVSVRDTTQVPTDAVAVDWGGQLDHVLEAA
                     RETLGLGPRTRLTAELHSMLVYAKDQFFASHQDSEKHDDMIASLVVTLPSAHTGGELV
                     VHGKGGSTSYRGSRQEPIAVVLYADLRHEVQPVRSGHRISLTYNLIRHQSDEPDAAAG
                     PVGDVALLLERHFTAPVPARWRGDDVTPPTRLVYLLDHEYTPRSLTWKALKGADITRV
                     ATLRAAGTRAGCEVVLALADVHETWQDDVYFDDDFGGRRSRRRGGGVDPDPHQLIDSE
                     VTLTHWRGAWARGTEEISDYVDGREVCASTPTVRLTPYESEHEGYMGNYGNTVDRWYH
                     RAAVLVWPVRLQFVNRAQVSMAWAVADLQDHVDKGEADIARDDLVSMMPFWHSQIGGL
                     DPAPKLVDEALTLAADLDEPDLARTFLGTFRIDVLTTAVAPRLLRLAETYGDVWAKDL
                     VTAWSTNSRRRGTGTADPVWLAGLPSLAMALCDSEILPRAMVELAWDGVRPRITPLLA
                     ADLTSRVRGQLETLAGSLTSVLHAAAVCRLDAVADEVLASCRQGEPALPLLIPLLDAA
                     ANWPAEDRREARVGEAAAYAAQLLAQRLERPARRLDDWSVPAPSACDCDLCGQLAAFL
                     SAPDERTLEWPLAGPNRRHIHSRIEASELPVTHTTRRQGRPFTLVLTKTAALFEREID
                     ARGDDELALARVQLLMD"
     assembly_gap    16300..16399
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16554..16811
                     /locus_tag="PKU75_04945"
     CDS             16554..16811
                     /locus_tag="PKU75_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPF80257.1"
                     /translation="MTASEASRNFSAVLDAAEEGETIVVTRAGRRVATIAPAPTVNAP
                     ELRAVFSRWQGDPAFDDSLEARIEAARSSVLADLDNDPWLD"
     gene            16799..17206
                     /locus_tag="PKU75_04950"
     CDS             16799..17206
                     /locus_tag="PKU75_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPF80258.1"
                     /translation="MARLILDTGVLIAGVRGRLDLGALADADDVAIPAIAVAEYLAGV
                     FLDPPSDRASAQRAFLADVLAVVPVKGYDIDIAEQHAWLLAHTHRTGTRRGTHDLIVA
                     ATARATDRIVLTTDERARFADLPEVRVRLASRT"
     gene            complement(17390..19183)
                     /locus_tag="PKU75_04955"
     CDS             complement(17390..19183)
                     /locus_tag="PKU75_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80259.1"
                     /translation="MAKGKGKGRRTPHRSATRGRAAVRRHPAAPAPEDLELARGLREA
                     MRGAPLSTATLVSQMIEYTEAGDDEGAPPLAAAPTLANLVESLIGIPIAETTAALHVM
                     AILLTDEVMAARIRRELAGRKHPMPIDVAELASLRITRAVTMTIPGDVGEDLMLELTG
                     PGVRDVTLMTYVDFRGGPFLKDAFLMPSSLEQAKAQMREIAERDGFAPDYPEVSLADA
                     RARLEAAMATYGSLEHMLEPQEMWPGLRPLLRFLLAHLPTGGYGYPGDAGIPMDLDDV
                     GLDPLDEEFEEEVHDLAHAFLAAYLGSEEAEAIGDDDLIHDVAHEFAAIIVGEDEQYS
                     WETIDIERVMTRVLPPLMPAELKEYCRAPEVLQAFVRHCHAVAGVEEAVDFRVGMEIA
                     RWVPDYLRMREDPGVVAIRNALETAALAADSIGLSYEDPAVEILGSVEALATLDDEPL
                     PDEPLDVSGVPADIVERVSRMSALADGAIARLYDDVELRTAARRLIAKVAAGDPAIFR
                     RKSGDEMGAAALVWLVAKANDQIGESWTLTVGELMDHFGLKGSPSQRAQPMLAAIGGS
                     TDYDGRVRLADPLLLTSETRAELLDWFGDPH"
     gene            19317..20315
                     /locus_tag="PKU75_04960"
     CDS             19317..20315
                     /locus_tag="PKU75_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HPF80260.1"
                     /translation="MKTGRGRAAGAPAELRQVLWQAPGGALGEILGQFGELALIADAA
                     EVAVIAETLQRGEHTSGEAPLAIGDWVCSHSRRYPTGATCARSVKLARHIAKKVLPDR
                     LAEAVLSGQAPVAPAAVAADEMQRLLPDLQPGFVAAGWDAYTELALAGDVGEVRKLRP
                     ALIAKYGDGDKGKDEERDRSRACLSGGASDGGALVDYRLSLDAESAAVLEGALGSLTR
                     PVPGPDGEPDPRPWTNRRAHALIDLLNRATKKSDGDVVLTRSSTHTTVIVDAPSPAAP
                     GLRTGPMHTTRSTGPTVGIPISTTQPLLCRMHHGVVHSRHLAATVTTGPPGSTNND"
     gene            20668..>20852
                     /locus_tag="PKU75_04965"
     CDS             20668..>20852
                     /locus_tag="PKU75_04965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80261.1"
                     /translation="MPDAVWSFRRVTREDFPLVASWLAQPEVHRWWQHEWSAAAVERD
                     FGSSVDGAEPNEDWLALG"
BASE COUNT         3074 a         7135 c         7323 g         3120 t
ORIGIN      
        1 ctgtcacccc atcgctctgt caccccatcg ctctgtcacc ccatcgctct gtcaccccat
       61 cgctctgtca ctccatcgct ctgtcaccca tcgctctgtc aggctgacga catggacacc
      121 atcggcctgc tcgccgagca actcgactgg cactggacca accagctgcg tccgcgattg
      181 gccggcctca ccgaccagga gtacttctgg gagcccgcac ccggggcctg gaccgtgcgc
      241 ccgcgcgacc cggcggccag cggcccgcaa cccggctcgg gcgcgatgac catcgactgg
      301 gcctaccccg agccggtgcc gccgccggtc accacgatcg cctggcggct cggccacatc
      361 atcgtcggct gcctcggcgc ccggaatgtc gcgcacttcg gcggccccgc ctgcagttgc
      421 gacacttttc tgtatgccgc gaccgcggat gaggctcttg cgcaactgga ttcggcgtat
      481 gccgggtggc tggccggcgt gcgcggcctc gacgacactg ggctggccca gccgtgcgga
      541 cccgccgagg ggccctgggc cgacctgccc atggccgcgc tggtgctgca catcaaccgc
      601 gaggtcatcc accacggcgc ggagatcgcc ctgcttcggg acctgtggcg ggcgcgatga
      661 cgcggatcgc cgtcgtcggg ggcgggatcg ccggactgag cctggcggct gccctcgacc
      721 cggacgcgac cgagaccacg gtgtacgagt cgcagccgga gcgttccgcg ctgggcgccg
      781 gactggtgat ctggccggcc gcccttcggg cgctcacgcg gatcggcgcc tgggacctgc
      841 tgcgtgccga cgccacgccc gtgcctcccg cggcgatccg cgcgctctcc ggccggatcc
      901 tgctacgcac ccgcgagacc ggcatggtca tcgtgccgcg gccggcactg ctcgccgccc
      961 tgcacgccgt cctgccggac tcggtgcagg tggtggcccg ggaggtgacc gacgcccgag
     1021 cgctggacgc cgacatcgtc gtcggcgccg atggcgtgcg tagcgtcgtg cgtgggctcg
     1081 tggcaccgcg ggtggccgag cgccgagcca cgccgtatgc cgccctgcgc ggcacccgca
     1141 gcacggtgcc tgtcgaggta gctggcgaat actggggcag gggaggactt ttcggcatcg
     1201 tgcccatggg cgctggcgcc tactggttca cgacgcaccg cagcggtctc gggccggagc
     1261 ccctggacgc cggggcgctg atcggcgagg cccaggcggt tttcgccggg gccgcgccgg
     1321 tcatcgcaga gaccctcgcg gcggcggcgc ccgacacgct ggccacccgc ctgtggatca
     1381 cgccgccgct gccgcgctac cggcgtgacc gctatgtcgt catcggcgac gccgcacacg
     1441 ctatgacacc caacctcggt cgcggcgcct gtgacgcgat cgtcgacgcg gtcaccctgg
     1501 ccggcgccct gcgcaccggt catgtggccg cctgggaggc tcgccgcctg cccgtgaccc
     1561 aggcggcccg agcgctctcc ggcggcatca tgcggatggc gttgtccggc ataccatctc
     1621 gccgccgctg acccacgagg actcgtcaat ccgcggtgac cgcccggtgc ccggacggag
     1681 cccggccaca cgaatgcgcg agagtcacgt cacgatcagc gttcacgggc cccggccagt
     1741 ggcactgtga tgaatgtgac tgaggacaat cgagcgacgg caccgcggac ctgccgggac
     1801 tgcggtcagc tcctgaacgc cacacccgac ggctccgaga cggccgacga cgcggccgat
     1861 ctggactgga ccgacttctg tccgagtccg gactgccccg tcaagctggg cgaggccgcc
     1921 ggataggcct cctgagtgca gcgtgggcgg tcgctggcct cagcggcccc cgtccgtcag
     1981 gatgaacgcc gggatcccgg acgggtcccg ctgcggacgg aaggcgagcc ggtgttcgag
     2041 ttgagcgacg aagagctggc cgttgtcgag ttggtgcgcg actgggtcga ccgggaggtc
     2101 aagccggtcg cgtccgaact ggagcatgcc gatcgctacc ccgaggcact catcgagcag
     2161 atgaagcaga tggggatctt cgggctggcg atcggcgagc cgtggaacga ggcgggtgtc
     2221 tcggcggcgt gctatgccgc cgtcaccgag gaattggccc gtggctggat gagcctggcc
     2281 ggcgcgatgg gcgggcacag cgtcgtcgcg aaactcatct ccacgttcgg caccgagcag
     2341 cagcaggagc actacctgcc gcggatggcg accggggagg tgcgcgcgac gatggccctc
     2401 accgagcccg gcggtgggtc ggacctgcag gccctgcgca ccacggcccg ggccgagggc
     2461 gaccactatg tcgtcaacgg cgccaagacg tggatcacga acgcgcgccg ctcccagctg
     2521 atcgcacttc tgtgccgcac cgatccgcag gcggacccgc cccaccgggg aatcagcatc
     2581 ctgctcgtgg agcacggcgc tggactggcg gtctcgcgcg acctgcccaa gctggggtac
     2641 aagggggtcg agagttgtga gctggccttc accgatttcc gtgcgccgcg gtcggctctg
     2701 ctcggcgccg aggagggccg cggtttcgcg cagatgatgc gagggctgga gatcggccgc
     2761 atccaggtcg cctcgcgggc gctcggcgtg ggccgggcgg cgttggagga cgcgctgcgc
     2821 tatgcccagg agcgccagtc gttcggcaga cagatctggg agcaccagtc ggtcggcaac
     2881 tatctcgccg acatggccac cgcactgacg gccgcgcgcc aactggtgca gtatgccgcc
     2941 cgctgctacg actccggacg ccgctgcgac atggaggccg ggatggccaa gctcttcgcg
     3001 tccgagacgg cgatgacgat tgccctgaat gcgatcagaa tccatggggg atacggctat
     3061 tcgacggaat tcgacgtgga gcgctacttc cgggacgcgc cactgatgat cgtcggggag
     3121 ggcaccaacg agatccagcg caatgtcatc gtcaaggaac tcgtccggcg caaccttgtg
     3181 gcatcgtgac cgccgctgaa cccggatcgc ccccgatcgt ggtggagcgg aacgagatcc
     3241 tcctgcccgg tccagcggcc gcgctcgccg ggctcctcga tgtcgccgac cccggcctcg
     3301 aggccggcgg ccggctgccg atgctgtggc attggctcta caccctcgac cggccgcccc
     3361 ggaaggacct cggcaccgac ggccaccccg tccgcggcgc cctccccgcc ccgccggaac
     3421 cgggacgccg gcgaatgtgg gccggcggcc gggtgaccac ccacgcgccg ttacacgcgc
     3481 acgtgccggt gcgacggcat acccgcctcc tctcggaggt gacgaaggaa ggccgcacgg
     3541 ggacactgac attcgccacg gtcctgcacg agatcagcca ggacgaccgc gtcgcggtgc
     3601 gggaggagca ggacatcgtc taccgggccg cgcccactgc ccccaccgcc gatgggcgac
     3661 cgcccggcga cccggagccc ccgctccccg ttgcggccga cgagtgggcg atcgacgtcg
     3721 acccggtgct gctcttccgc tactcggcgc tcacctacaa cggacaccgg atccactacg
     3781 accgcgacta cgcgcgggac gtcgagggct atccgggtct ggtcacgcac ggacccttgc
     3841 aagcggtctg tatggcggag gctgcccgcc gcggcggcat cgacgccagc gtgggttacc
     3901 acctcgaata ccgtttattg gcaccgcttt tcgaccacca aggcttcgtc gtccggtgcg
     3961 cgccggcctc gaacggggcg gacctgagcg tccgcgaccg ctggggccgc cagaccgccg
     4021 cgggagtcct cgcccgtctc tgacgtgcac caggtaggtc gtgaccatcc tccgtcgggt
     4081 gaccaggcgt cagctggcaa ccgccgaccg gacggggtca gggcctgccc cgctgcatgc
     4141 gcggcccggt cccgtcgcac accatggtgt cgctgccgct gccggcccga tggccttgtc
     4201 ccagtgggcc attggcgcca cgcccgccct gcatgggccc cggctccatg ccgccgccca
     4261 tcgtggttcc gttgacggcg gcggtgaagg cagcgaggtg ctgttgcgac gcggccagca
     4321 gctggctcag gacgttcttg acgtcggtct tggtcgtggc cgcgatgctc ttcttcaggt
     4381 ccgcgatgtc ccgcttctcg agggtgacac cgaccttcgc cgcctcggtc cgcgaaatca
     4441 gcccttgcgc cttccaggtg gtgtagagct tctggagggc agggtcggcg tagcttccgg
     4501 caggacgccc cgcggagggg tccgggatcc ggtatgtggt gaggaggcgg ccgatcgcct
     4561 cgaagtgccg ctgctcggag agcacgatcc gggagaacgg ggcggcgccg ttgtacgtgt
     4621 tgctgaagag ccggtataga tcgcgcgcca tccgctcctc ttcgcggttg aaggagagta
     4681 tggcggcgag ctgggcatcg gcggtcgatg tggatgcggt cctgctcgcc tcgtcaaatg
     4741 cgatgctccg ggcgccggcg gcgccggccc cggtgagtcc cagggccgcg gcggccacga
     4801 tcgcgacgac agtggttcgg gtgctggcaa cgccctgcgt gccgtcgcgc cgcggggcag
     4861 gatcgtggtg cgcagcatac ggtccggcgc ctcggcgatg ctgactgtca ccgacgacgg
     4921 cgaaggcctc gacagcgccg atctcgagcg gatcttcgag cgcttctacc gggttcctgg
     4981 gcgccgaagc gtcgagggtg aatccggaca cgggatcggg ctgacgatct ctcgggcctt
     5041 ggctcgcggg cacggcggcg atctcacggc agcctcgcct ggcgccgggc agggagcgac
     5101 cttcacgatg accattccgc tgcgcgacga cgcgtgaccc acggctttgc gagagcctgg
     5161 cacggctcgt catcagcggg aatggtcacc ctcgcaactc atcccgccat gcgcccgcgc
     5221 tcgccgcgga cggttcgagc acgacgacgg gtgtgggggc ggagcgggcg gccgcccagg
     5281 cgccgaggtt ggggtcaacg gcgagccagc gcgcccagat cgcctcccgc tctgcgcctt
     5341 tcgcctctcg cgcaacaaca tctcgtgccg gtccgccctt gagacggatg gtggcggagg
     5401 gctgagcgcg caggttgagc agccaggcgg ggtggccggg tagccacccg ttcatggcga
     5461 cgaccgacca gccgtcgccg tgcgtcaggt acgcgaggat gacgctgcga tcggccccgg
     5521 accgacgccc ggtggtcgtc aaccgcaagg caccccaccc gcgtttgttg gccggcgtcc
     5581 agaggaaccg gccgccgctg acacgataga gcaggcgatg gatccgccaa gcgctcttga
     5641 tgaccggtgc cggaggcagg cggggcgggg cagtggcagc accgagggtc ggagtcgaag
     5701 acatacccac agcttcgcga tgccccggcc gccgggccat acgggtcagc cctgaccgag
     5761 cccgcctcgc caccgtccag gccgccgcgc cgcccaatcg gtgagccacc gagctgctgg
     5821 tcgtggggct tcctcggtca gttggcgccg tcgggaaggg cgccgaggcg caggaagacg
     5881 gtctcgccag tggcgtcgtc gagaccgtcg ttgtcggtga cggcgaccat cgtgccgccg
     5941 gagacggtga aaccttcgag cttctcctgg gtccacccgc gcgtcgagcg caaggccggc
     6001 agcacgtcga cgacgaggcg cttgggcaga atcggcaggt tcaccgcggg agcgcccttc
     6061 gcggggacgt cgacggcata tacgcgcttg acacgggcgt tcgggccatt gagcttgtcg
     6121 cgctcgatga ccgcgacggt gttcgcgtcg agggcggtga tctccgacag gcccatccag
     6181 tcgccgtcgg tggtcgtggt ggtcagccgg taaccgaacc acgaccaggt gccggtagcc
     6241 accgaatagc gcccgatgcg ggcgacgttc tcgccgtcga tgggaccgtc ggcgccggcc
     6301 gggtcggccc acagcggccg ctgcagcgcc acgtagacaa cctcgtcggc gccggtgccg
     6361 atcgcactga caccctcgat gccccacttg ccgacgtgcg cggcgatatc ggccggcagg
     6421 ctcacgcgtt gctggatgcg gccggcgctg gtggtgcgca ccaacgcgtt ctctgcgccc
     6481 gtcgccccct cgacgccgag ccagtagcca ccggccgcac gggccgaaat gccttccagg
     6541 tcaaggccga cggccacgcc ggactcgtcc gtgacatcga ccgcggaggt gatcagcgcg
     6601 ggccgtttcg cggtgttgat ctggtagatc cgaccggtcg cgtatgccga atcgctcacc
     6661 gcagcaaggc gattcgtcgt gcccgggatg gcgctcagcc cggagagcgc gccccagccg
     6721 atgggtgccc ctccggcata cgcggacacg atcgtcggga actccagccg gttgtccgga
     6781 ccgccaagtt tgaagaggtt gaccgaggag cgcacctggg cgtccgagtc gtccacctcg
     6841 gaggagacgg cgagcagtcc ccgcgacggg atcggcagga cgccctcagg gccgttggtc
     6901 gtcggcagca gctgccggaa caccggcgac atgggtttcg tcatgtcgta tgccgcaacg
     6961 aaattcgagc gctcagaggc gacgaaggca taccgcttgc cgcccatggt cgccaccgcc
     7021 aagccctccg gttcggtgcc cttgttctcc gcccggccct cgttgtgcag gccgtacttc
     7081 acggccaggc gctccaggct gacaccggag tcccaggcga tgcccttggt cgcgctgtcg
     7141 aagacggtcc agccccggct gccgcccttc cagtcgccct cgttggcggt cgcgaggtag
     7201 cgatcgtcca cccaggcaat ggcatcgggc tcgcggggca cggtgatcgt gccggtgagc
     7261 gagatattgc cgtcctcgag ggtgtcgaca cgcgtgcgcg tcacgatgcc ggccgaccag
     7321 gccttggtga ccttcttcgt cgccaggtcc accaggacga tgccgttgtt ctcctgcaac
     7381 gtgaccgccg cgacgccgcg cgagttgatc gtcacgtatt ccggctccgg gtcccgcggt
     7441 gtgtccaggc cggcggcggc aagcatcgga atcggcgcac cggtcgctgt cgtcaacggc
     7501 accttcgtcg ccacccacga cgacgggctc gcggtcggca tgtcgacgac ctgaaggaac
     7561 ccggcgggca actgaggcag ctcaccgtcg ttgacgtcct cgtcgcgctc gttctcgatg
     7621 gcaatgatcg cgcggcgctc gtcggcgctg acgtcgatcg aatcaggttg tcccccaaga
     7681 gcaatggttc gcaccacctt gcggtccgac aaccggacga cgacgagctt gccggacggc
     7741 tcgacatacg aggcgctcgt gtcgaccacc acgaggacga agcccccgac gacgtgcacg
     7801 ctggtcggct ctgcgtctgc gtcacccaac tgctccatcg acagcgtccc gaggccgttg
     7861 ggtgcactcg cgttgctgat gtcgacgaac ccgatccgtt tgcccgcggc atcggtgtag
     7921 atcagcgtgt gcccgtcctt gctcaccgtc gagatctccg caacggtctc ctccagcggg
     7981 tcgacgccgg cgggcacgtt ctgatacacg gggtaggtcg ccatccgcgc gaacggcccc
     8041 gtgcgggccg tctcgccgtc ggtgcttccc ccggcggcgg tcgcgccggc gaccatcgtc
     8101 aaggccaggg cggcagcgga aatggcgggg gtcttgcgca tgtcgtctcc tcgggcacca
     8161 acggttggtg cccggaagct agggtgcccg ggtggccgtg gccggaacgc cggaccaacg
     8221 gcgggggtat gccgtgcgtc gccttccccc agatcccgcc cgggtggcca ccggcgctga
     8281 cctggtcggt tgtccagttc tcgccgctcc gagccacgtc tctcggagac cggctggccg
     8341 gcatgtgctg gtcatcgtgc ggccatctgg atgccacgtg tgagggccac actgggccgg
     8401 catgacgacg ctgcgccgta tgctgccgcc cctcctcctg ctctccctcg ccgccccctc
     8461 cgcgatcgct gcctccgatg atgaggaccg tctcccgcgg ggggcggtgg atacccgtgt
     8521 cgagacgccc accctctatg acgacgatgc cggtggcaac gcctccggtg acgacccggc
     8581 gatctgggtg cacccgagtg actcgcgccg ctcgatcgtc atcgtcaccg cgaaggaggg
     8641 cggactgcgg gtgtacgacc tgcgtggccg ccaactccag aagcttgccg ccacccccgc
     8701 gccgcggccg gacggcgtgc ccggtcgctt caacaatgtc gacatcgccc gcgggctgat
     8761 ggtcggcggc aagcgcaccg acgtcgccgt ggtcagcgac cgctacaacg accaactccg
     8821 cttcttcgcg atcaacccgg ccggcgccgc agcgacccgg caacttcgcg aggtcaccgc
     8881 ccccggcgtg cccttcgtct tccagcacac gcgcgcgggc gtggacacgg agaagacggc
     8941 atacggcctg gcggtgtggc aacccacagg tggaccgacc tatgtcgccc tcacccagga
     9001 gggcaccacc cggatcgcga ccgtgcgatt gacgatctct cccaacggaa tcggctacac
     9061 cgactcccgg cacctgcctc tgccggccag cttcccgctg cccaatggca cgacctggag
     9121 cgtatgcaac gagccgggca cccggccgca gctggagggc atggccgtcg acaacgcccg
     9181 caatgtcctc tatgccgcgc aggaggatgt cggcctgtgg cggatcccgc tgccgctcta
     9241 ttcggggcag ccgaagctca tcgacaaggt caccgacttc ggcatcaccg accgctggga
     9301 cgaggagagc gaggagtgcg taccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnncaccg acttcggcat caccgaccgc tgggacgagg agagcgagga gtgcgtaccg
     9481 gtcgaccccg accagcctgc gacctacgcc ggacacctgc tgacagccga cgccgaaggc
     9541 gtggcgatcg cccatgccgg ggcgggcggg cagatcgtcg tctccagcca gggcgacgac
     9601 cggttcgccg tctacaacct caccggagca aatagaccat tgggcagctt ccggattcgc
     9661 ggcatcgacg gcgccgacaa tgtcaacggc tccgacggcc tggacgtcgt caccgtcccc
     9721 gtggctggct atcctcgcgg acttctggtc agccacgacg aacccgacag cggtcccggt
     9781 gtcgaccccg agcgggaccc caccaacttc tcgtacgtgc aatggggtgc cgttgtcgac
     9841 gccctcgccc tcccacgctg agcgacatcc gcggccgtgg tcagccgatc accggcgcgc
     9901 gctgcagatc gttcaggtcg gggttgacgg cggcgaggag atccatgaag gcgcccggcc
     9961 catgcgcgtc gatgaagacg gcctcgctcg cgctgatcga ggggtccccg gcggcgaaac
    10021 ggacgagctg gaacggtcac gggtcccgtc gagcagctca tggcggcgcc cgtcaacggg
    10081 catcgcatcg agatcggccg agtgaactcg cggtccgacg tcaccacgcc gctattcgtc
    10141 gccgcgcagg gtgaacgagc gcagccggtc gccggtgagc cagagggcgc cgagcgtcac
    10201 gaccagggcg gcgaggacgg catacgacaa ggggaggaag gcgtcgtcga ggcgggcgct
    10261 gaaccggtag ccgacctgtc gcccccaggc gccgatcgag acccacttga tgccggtgag
    10321 caggccgccg agcaggccct cccacatcag cacgaacagc agccccgcca cgacggcgtg
    10381 ccgggttacc gccgccagcg ccagaaacag ggccgagtat gtcgtgcccg ccaccgcacc
    10441 cgccgcgccc cacgcgagcg cgcgccgcgg gttgcccgag tccatgatca gccctgccac
    10501 gaccagcggc agtgccccca gcgcgagagt ggcgagccac gccacgacga acttgctcag
    10561 cgcgatcacg tgccgggaca ccggtttcgc cagcagatag acgatcgagc cgtcgtcgat
    10621 ctccggcccg agcaccgcgc tcgcggcgag caacgcgacc agcggcagcg cgagcgtgta
    10681 gccgagggtg aggacggcgt catacccgcc gtcggcgtcg gtcaggacgc gcagcaacac
    10741 cgccagcccg atcaggagag cggggatgac gagcagcacc acgccgcggc gtcggccgag
    10801 gagggcctga gcggagagtc gggcgatggt ggggttcatg cggggccgcc gacgaggtag
    10861 gcgaacacgc tctcgaggga ttcgtcgctg ggtgtcagtt cgaggatccg tatgccgccc
    10921 tcccgggcga ggtggggcag gcggacggcg aacgcgccca ggtcggcgac ggagacgtcg
    10981 agcccgccct gccgacgcag ggtgacgccg aggacggagg gctcggtcat gagcgcggag
    11041 gcgagacggc gatcgtcgga ggaggccacg gcatactgca tcggccggtc ggtcatcagc
    11101 cggcggatgg tgccgaagtc gccggaggcg gcgtgccgcc cggagacgac gacctcgatg
    11161 tggcgggcga cctgctcgac ttcttcgagg atgtgggagc tgaacagcag cgtgcggccc
    11221 tcctcgccca tccgccgcag cagtgccatc aggtgcatgc gttggcgcgg gtcgacgccg
    11281 ttgaacggtt cgtcgaggag aaggacggcc gggtcgtgga cgagggcgga ggcgagcttg
    11341 gcgcgctggc gcatgccctt ggaataggtg tcgatgcgcc ggtcggcggg gtcggtcatc
    11401 tcgacggtcg cgagggcgcg ttcggtggcg gcgccggggt cgggcaggcc gtgcagttcg
    11461 gcgttggcgc gcacgaactg gcggccggtg agatagccga agctgagttc gcgttcgggc
    11521 acgagcccga tggtgcgata ggcgtcggta tgccgccaga tcggcttccc gtccaaggtc
    11581 accgtgcctg cggagggggc gaggaagccg gccatcatcg cgatgagggt ggtcttgccg
    11641 gcgccgttgg ggccgagcag gcccgtgatg ccggggccga cggtcatcga gatcccgttc
    11701 accgcaacga cattcccgaa ccagcgggag gcggcgtcga gagtcaggac actcacttcg
    11761 tcgccagctt acgatagcgc cagactagcc cggcgacgca ggcgagggcg atggcgagga
    11821 ggacggcggc atacagccac tccatcgcgg tcgagctcgg cggggtcatc gacgacccgt
    11881 cggcccacgc ggtgacgagc ccgcgataga gggagtaggg ggagaacagg ccggcgatct
    11941 cgccgatgcg ctcgttgccc tcctgcgacg agatcccctg gatggccgcg acgatgccgt
    12001 cgccgaggag gaggaaggcg atggtcgcca tcaccccgaa cccgcggcgg gtcgagaccg
    12061 acgcgatgag tccggcgatg ccggtgatgg cggcggtcag cagcagcgca agcccgacgg
    12121 cgacggcggc tttgcgggtc tcgtcggtga ggtcgaattc cccaagcagc gcaacggcat
    12181 acaggatcac gatcggcagg atgaggaaga cgagggtggc accgagcagg gcgagccagc
    12241 gggcgatcgc ataggcgggc gcggggagtg gccgggcgag atagagcgcg agggtgccgt
    12301 ggcgctggtc gcgggagaag agcacgggcg cctgggcggc gacgaagatg ccgagaagga
    12361 cctgggtggt cgcggcatac cgcgcgtagc cgatcggcat ctccttgagg ttggcgagca
    12421 tgatggcgcc cgcgatcgcg agggccgggg cgagattgaa ggcgagcagc acgaagggga
    12481 ggattttgct gcggccggag cggccgaggc cgaaggcgtt gcggaagccg gtggtcacga
    12541 gcgcgcgggc gattgcgccc tcgccgtggc ggggcccggc atacggtcgg tagccgatgt
    12601 cgtggatgac gctggcgctg ttggggtttg cgggtatgcc gcccggctgg ctcatcgtgg
    12661 tccgctcgtg tcggcgaaga cgtcttcgat gcggcggtgg tcggcctgga ggcggacgag
    12721 gccgaggccg agcgcgccgg cggtgtcgcg gatgacgtcg tgcacgtccg cgtccgcccc
    12781 accgtcctgg acgccggcgg gatgggaggc gtcgacggtg agcatgctgc cgcggcgtgc
    12841 agcggtcagg ccggcgcggg tgagggcatc cagcatccgg gcctgagcgt cgtcgccgag
    12901 gacttcgacg agcagggcgc cggtggcgtc gaggaagtcg tcggtgcgcg aggcccgcag
    12961 cagccgcccg ccgtcgagga cgacgacctg gtcgctgacg cgttcgagtt cgccgagcag
    13021 gtgcgaggtg acgatgatgg agatgccgaa gtcgtgaccg atgcgctgga tgaggccgag
    13081 catctcgtct cgggcggcgg ggtcgaggcc gttggtgggt tcgtcgagga agacgagctt
    13141 ggggtcgtgg acgagggcct gggcgagctt ggccttctgc ttcatgccgg tggagtagcc
    13201 gcccatgggt cggtagcgct cctcggcgag acccacgtgc cgcagcacgt cggcggcgcg
    13261 ctcgcgggcc gcgacgcggg ggaggccgga catcatggcc atgtggacgg cgaagtcgct
    13321 ggcgctgaca tccggtggga gacagtcgtg ttcgggcagg tagccgacgg cggcgcggat
    13381 ctgcgggccc tcggtagcga tgtcatggcc taggacgcgt gcccgcccgg cggtcggcgg
    13441 gataagaccg aggaggatct tgatgagggt cgacttgcct gccccgttcg cgcccaccag
    13501 cccggtcacg ccgggagcga cctgcaccgt gaggccgtcg agcgcgagca ccgcgccgta
    13561 atccttggtc agcccctccg tgaccaccac ctggtccatg ggggcaatgt atctggtggg
    13621 tctcgatcgc ggtgctccgg cttggacccg ggggacgtgt tggcgcgctc gccggacctc
    13681 cattacccgt ccacgtcacc gtcggcggac gaagggcgag gctgacgcgc cgtcaatccg
    13741 ataggcacgc gagtggcacc accgtgacgc cgtcgggcct ggtgaagagg tggggtcccc
    13801 gagccctggt agtagccctc ctgccgaata tctactctac ctcttgccgg atatctaatc
    13861 gacctggcgt cgagaagcca actcgacctt ctgccgatcg gggcccgcgt tcgaccgcca
    13921 ccgatccagg agtcgtgcac acggttgagg ctctcccgtc catctccgac aatcgtctgt
    13981 atgtcgattg cgcccaaaga cgagatggca cgactgctcg cgggcgaacg tcccacggtg
    14041 cgtgggcacg ggcagctgcg agtcgacctc gacgacctcc gcgtcgaggt cgccgggcta
    14101 ggagagttgc gtcagccggt gacggcgtcg acggccaaga agcttgcggc gctcggcaaa
    14161 cccgcggctt tcgggctcgg cacgcagacc gtactggatg tcagcgtccg cgacacgacc
    14221 caagtcccca ccgacgcggt cgccgtcgac tggggcggtc agcttgatca tgtcctcgag
    14281 gccgcccggg agacgctcgg gttagggccg cggacgcggc tgactgccga gctgcactcg
    14341 atgctcgtct acgcgaagga ccagttcttc gcctcccacc aagactccga gaagcacgac
    14401 gacatgatcg cctctctcgt ggtcaccctc cccagcgcgc acacgggggg cgaactggtc
    14461 gtgcacggca agggcgggag cacgtcatac cgcgggagcc gccaggaacc gatcgctgtg
    14521 gtgctgtacg ccgacctgcg ccacgaggtg caaccggtcc gctccggtca tcgaatctcc
    14581 ttgacctaca acctgattcg tcatcagtcc gacgagccgg atgctgcggc gggcccggtg
    14641 ggcgacgtgg cgctcctgct ggagcgtcac ttcaccgctc ccgtgccggc gcggtggagg
    14701 ggcgacgacg tgaccccacc cacgcgcctg gtgtatctgc tcgaccacga gtacacaccg
    14761 cgcagcctca cctggaaggc cctgaaaggc gcggacatca cacgagtcgc cacgctgcgg
    14821 gcagcgggga cgcgggccgg gtgcgaggtc gtcctcgcgc tggccgacgt ccacgagacc
    14881 tggcaggacg atgtctactt cgacgacgat ttcgggggcc gccggtcgcg gcggcgcggc
    14941 ggtggggtgg atccagaccc gcaccaactg atcgattccg aggtcacgct cacccattgg
    15001 cgcggcgcct gggctcgcgg aactgaagag atcagcgact atgtcgatgg tcgcgaggtg
    15061 tgcgcctcga cgcccaccgt gcgcctgacg ccctacgagt ccgaacacga gggctacatg
    15121 ggcaactatg gcaacacggt cgaccggtgg tatcaccggg ccgcggtcct cgtctggccg
    15181 gtccggctgc agttcgtcaa ccgtgcccag gtctcgatgg cgtgggcggt cgccgacctg
    15241 caagaccacg tcgacaaggg cgaggccgac atagcccgcg acgacctcgt gtcgatgatg
    15301 ccgttctggc acagccagat tggagggctt gaccctgcac cgaagttggt cgatgaggct
    15361 ctgacgctgg ccgcagacct ggacgagcct gacctggccc gcaccttcct cgggacgttc
    15421 cggatcgatg tgctcacgac agccgttgca ccgcgcctgt tgcgactcgc cgagacgtat
    15481 ggcgacgttt gggcgaaaga cctggtcacc gcgtggtcca ccaactcgcg gcgccggggc
    15541 acggggacgg ccgatccggt ctggctggcg ggcttgccgt cactggctat ggcgctgtgc
    15601 gacagcgaga ttctcccccg agccatggtc gagctggcct gggacggcgt gcggccgcgg
    15661 atcacgccgc tgctcgccgc ggacctcacg tcacgggttc gtggccaact cgagaccctc
    15721 gccggctcgc tcacgtccgt cctccacgct gcagccgtat gccgtctgga cgccgtcgcc
    15781 gatgaagtcc tggcctcgtg tcgccaagga gaacccgcgc tcccgctgct tatccctctc
    15841 ctcgacgctg cggcgaactg gccggcggaa gaccggcggg aggcccgggt gggtgaggcg
    15901 gcggcatacg cagcgcagtt gctcgcccag cgcctggagc ggcccgcccg ccgcctcgac
    15961 gactggtcgg tccccgcccc gtccgcctgc gactgtgacc tgtgtggcca actggccgcc
    16021 ttcctctccg cgccggatga acgcacgctg gagtggccgc tcgccgggcc gaatcggcga
    16081 cacattcact cccgcatcga ggcctcggaa ctgccagtga cgcacacgac ccgtcggcag
    16141 gggcgaccgt tcacgctcgt cctcaccaag accgccgccc tcttcgagcg ggagatcgac
    16201 gcccggggcg acgatgaact cgcccttgcc agggtgcagc tcctcatgga ctgagagcga
    16261 gtccgccgcc aagcttcgac gagcttgtgc gggcgggatn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnc atggactgag agcgagtccg ccgccaagct tcgacgagct
    16441 tgtgcgggcg ggattgcctt gtgccgaagc cggcgatgca cgcccatgtt gtcgaacaga
    16501 gccgtttcgt cgcgttgcgg gtgtcgatca gatattctga tcttgtgcgg gagatgacag
    16561 catccgaggc ctcgcgcaac ttctccgcgg ttctcgacgc cgccgaggag ggcgagacca
    16621 tcgtggtcac gagggcgggc cgacgggtgg cgacgatcgc ccctgctccg accgtcaacg
    16681 cgcccgaatt gcgcgcggtc ttctcccggt ggcagggcga tccagccttc gacgactccc
    16741 tggaggcgcg catcgaggcc gcccggtcga gcgtgcttgc cgacctggat aacgacccgt
    16801 ggctcgactg atccttgaca cgggtgtgtt gatcgccggg gtccgcggtc gtctcgacct
    16861 cggggcgctc gccgacgccg atgacgtggc gatcccggcg atcgccgtgg ccgaatacct
    16921 cgccggtgtc ttcctcgacc caccctcgga cagggcgagc gcgcagcgcg cctttctggc
    16981 cgatgtcctg gccgtcgtgc ccgtcaaggg ctacgacatc gacatcgccg aacagcatgc
    17041 ctggctcctg gcgcacacgc accggacggg cacacggcgc ggtacgcacg atctgatcgt
    17101 cgccgcaacc gctcgtgcca cggatcggat cgtgctcacc accgacgagc gggcgcgctt
    17161 cgcagacctt ccggaggttc gagtgcgact cgcctcgaga acctgacccg atccaaatcg
    17221 aggacgtcag ccaacccgcc gaaggaaaag cgacaaaccg tcgcctctag gatcactgat
    17281 cctgttcccg cgcccaccaa gtaacgcgct ggtggggaac tggcatgggc gccgccctgg
    17341 cgccgcagtg gcgacccgtc acccccgcct tgccgcgcct atgggtacgt cagtgggggt
    17401 cgccgaacca gtccaggagt tcggcgcggg tctccgaggt gaggaggagg gggtcggcga
    17461 ggcggacgcg cccgtcgtag tccgtgctgc ccccgatcgc ggcgagcatc ggttgcgcgc
    17521 gctgggacgg gctgcccttg agtccgaagt ggtccatcaa ctcgcccacg gtcagggtcc
    17581 agctctcccc gatctgatcg ttcgccttcg cgacgagcca caccagggcg gcggccccca
    17641 tctcatcgcc cgacttgcgc cggaagatcg cgggatcacc ggcggcgacc ttcgcgatga
    17701 gccgccgcgc agccgtacgc agctcgacat cgtcatacag ccgcgcgatc gccccgtcgg
    17761 cgagcgccga catacggctg actcgctcca cgatgtccgc cggcaccccg ctgacgtcga
    17821 gcggctcgtc gggcagcggc tcgtcgtcaa gggtggcgag cgcctccacc gacccgagga
    17881 tctcgacagc cgggtcctca tacgacaagc cgatgctgtc ggcggccagc gcggccgtct
    17941 ccagggcgtt ccggatggcc acgacgcccg ggtcctcgcg catccgcagg tagtccggca
    18001 cccaccgcgc gatctccatg ccgacccgga agtccaccgc ctcctcgacc cccgccacgg
    18061 cgtgacagtg ccggacgaac gcctgcaaca cctcgggcgc ccggcagtac tccttcagct
    18121 ccgccggcat caacggcggc agcacccgcg tcatcacgcg ctcgatatcg attgtctccc
    18181 aagagtattg ctcatcctcc ccgacaatga tcgcggcgaa ctcgtgcgcg acgtcatgga
    18241 tgagatcgtc gtccccgatc gcctcggcct cctcgctgcc gaggtatgcg gcgaggaagg
    18301 cgtgggcgag atcgtgcacc tcctcctcga attcttcgtc gagggggtcg aggccgacat
    18361 cgtcgagatc catgggtatg cccgcgtcgc cgggatatcc gtagcctccc gtcggcaggt
    18421 gggcgagcag gaaacgcagc agcggccgca ggccgggcca catctcctgt ggttcgagca
    18481 tgtgctccag cgagccgtat gtggccatgg cggcttcgag ccgcgcgcgg gcgtcggcga
    18541 ggctcacctc cgggtagtcc ggggcaaagc cgtcgcgctc ggcgatctcg cgcatctggg
    18601 ctttggcctg ctcgagtgag gacggcatga ggaaggcgtc cttgagaaag ggaccgccgc
    18661 ggaagtcgac ataggtcatg agggtcacgt ctcgcacgcc cggccctgtc agttcgagca
    18721 tcaggtcctc gccgacgtcg ccgggaatgg tcatcgtgac ggcgcgggtg atccgcaggc
    18781 tagccagttc ggcgacgtcg atgggcatcg ggtgctttcg gccggcgagc tcgcgccgga
    18841 tgcgcgcggc catcacctcg tcagtgagga ggatggccat gacgtgcagc gccgcggtgg
    18901 tctcggcgat gggtatgccg atgaggctct cgaccaggtt ggcgagggtc ggcgcggcgg
    18961 cgaggggtgg cgcgccctcg tcgtctccgg cttccgtgta ctcgatcatc tgggagacga
    19021 gcgtcgctgt ggacaggggc gctccgcgca tggcctcccg caggccccgg gcgagttcca
    19081 gatcctcggg ggcgggggct gccgggtggc ggcggactgc ggcccgtcct cgcgtggccg
    19141 atcggtgagg tgtgcggcgt cccttgcctt tgcccttggc catgtctcca tcccaccacg
    19201 ccgcagggct cgctcgctcg gatccggcgt ttcgcgggga cggtatgccg tccggtgcgg
    19261 gggtttgtgc aggtcggggg cgttgtcggt gggctgtgct tcactcgtgg ttgcacgtga
    19321 agacaggccg gggacgtgcc gcgggcgctc cggccgagtt gcgccaggtg ctgtggcagg
    19381 cccccggagg tgcgctcggc gagatcctcg gccaattcgg tgagctggcg ttgatcgctg
    19441 acgccgccga ggtcgctgtg atcgccgaga ccctccagcg cggcgaacac accagcggcg
    19501 aggcccccct ggccatcggc gactgggtgt gcagccactc gcgccgctac cccacgggcg
    19561 ctacgtgcgc gaggtccgtc aagctcgccc ggcacatcgc caagaaggtg ctgcccgatc
    19621 gacttgcgga ggcggtgctg agcggacagg cacccgtggc gcctgcggcg gtcgcggccg
    19681 acgagatgca gcggctgctg cccgacctgc agcccggctt cgtcgcggcg ggctgggacg
    19741 cctacaccga gcttgccttg gctggtgacg tcggtgaggt gcggaagttg cgacccgcgc
    19801 tcatcgcgaa atacggcgac ggcgacaagg gcaaggacga ggaacgcgac cgttcccggg
    19861 cctgcctatc gggcggcgcc agtgacgggg gcgcgctggt cgactaccgc ctgagcctcg
    19921 acgccgaatc cgcggccgtc ctcgaagggg cgctcgggtc gttgacccgg ccggtgcccg
    19981 ggccggacgg cgaaccggac ccgcggccgt ggacgaaccg tcgcgcgcac gccctgatcg
    20041 acctgctcaa ccgggcgacc aagaagagcg atggcgacgt cgtcctcacc cgctcttcca
    20101 cccacaccac tgtcatcgtc gatgcacctt ccccggctgc tcccggcctc cggactggac
    20161 cgatgcacac cacccgatcc actgggccga cggtgggcat accgatctcg acaacgcagc
    20221 ccctcctgtg ccggatgcac cacggcgtcg tccactcccg acatctcgcc gcgacggtca
    20281 cgaccgggcc accgggctcg acgaacaacg actgagagtg gtctgggact gacccccggc
    20341 agttatcaac acgccgtcgc cgcctaccga gcgggcccga ctgtcgcctg acacggctcg
    20401 ccggccaggg cttctccctg cccctgttgg cctgcctccc ttccgtccgg cgcacgggcc
    20461 tggctccctt ccggggtgcc tgtcagccgg cgggcctgtc ctgcccggcc ctgactcttc
    20521 cgtcccgcgg ccgggcgggc ggcctcccga cgaactcgcg gtgcgcccgc cctgcgccgg
    20581 ccggggtcga cagcgcgcat ccggcgcgtg atagctccgg atccggcaga cttccgggtg
    20641 gtgggtgtcg atgggggagg agctgagatg ccggatgcgg tgtggagttt ccgccgggtg
    20701 acgcgggagg acttcccgct ggtggcgtcg tggctggcgc agccggaggt gcaccggtgg
    20761 tggcagcacg aatggtcggc cgcggcggtg gagcgcgatt tcgggtcgag tgtcgacggg
    20821 gccgagccga acgaggactg gctggcccta gg
//
LOCUS       DAOPJG010000033        20796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2713_length_20796_cov_7.541777, whole genome shotgun sequence.
ACCESSION   DAOPJG010000033 DAOPJG010000000
VERSION     DAOPJG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 20796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20796
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_20796_cov_7.541777"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKU75_04970"
     CDS             complement(<1..661)
                     /locus_tag="PKU75_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPF80262.1"
                     /translation="MSDATKQTRYITCVLCEASCGLEVVTDGPAVVSIRGHEADPLSR
                     GHICPKAIALQDIHTDPDRLRTPVRRVGEPGADATWEPIGWDEAFDLVAEKIVATQKA
                     HGPDAVAVYQGNPNVHSLGALTHGVSLVRTIKSRNTFSATSVDQLPHQLIAMLLYGHQ
                     FLLPVPDIDRTDLLVLVGNNPMASNGSIMTVPDFAGRLRELKARGGRLVVIDPRRTET
                     AK"
     gene            745..1644
                     /locus_tag="PKU75_04975"
     CDS             745..1644
                     /locus_tag="PKU75_04975"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF80263.1"
                     /translation="MAGMDPFAAYTDHRLTRPDGRVVAWTQWGEPGGMPLLRVPGTPG
                     CRWAVRADTTPWAERGLRVITTERPGFGASTPLPGRGFLEHADDLAAILDHLGIAQVH
                     MIGGSGSAPHELAFAQQYPDRVRAATILVGAAPLEENEIDSMIPLNQQSHRLCRAGDR
                     EGEAALLGPIREAILADPLAAFRGIMEHAPADDQAVMRDPDWQAVFGRAQTEALGATL
                     DGWLDESMAMSQKWEEIVPEAITTSITWQHAAADANCPQSAARRLVDRLPNGRFIAWP
                     DDVGHLYGYHHEPEILDELLARA"
     gene            complement(1714..1866)
                     /locus_tag="PKU75_04980"
     CDS             complement(1714..1866)
                     /locus_tag="PKU75_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80264.1"
                     /translation="MVWILRKCVWWVPGFTSAAYEPQLRALDERLRSGEPFVAHSTRH
                     LIIAHR"
     gene            2034..2957
                     /locus_tag="PKU75_04985"
     CDS             2034..2957
                     /locus_tag="PKU75_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPF80265.1"
                     /translation="MTRRELHVVPVPGPGAEDVLIGHDGTAYTGCADGAIYAVRADGG
                     EPWRVGATGGRPLGLERLPEGRILICDAHRGLLDMALDSGEVRVLADRVDGIPMRFCN
                     NAAVSDDGTIWFTDSSTKWGIEEWKSDLIDDTCTGRLLKLLPGGQPEVVLDGLSFANG
                     VALASDQSFVVVAETGHRRLRRYYRTGPLAGNADVFAADLPAHPDNIAGGSDGLLWVT
                     YASPADATLARLQKSPKAIRTVVRRLPERLKPTPQRTVRVAAYDDLGQVVHDVETDAT
                     HWHMATGVREHDGRVWLGSLVEPALAWFDLA"
     gene            complement(2998..4191)
                     /locus_tag="PKU75_04990"
     CDS             complement(2998..4191)
                     /locus_tag="PKU75_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF80266.1"
                     /translation="MTERRVPSGLALGLVAIVLVSLNLRPGASSPGPLLEEIRAGLDM
                     SSALAGVFTGLPGLCFGLVGMVAVTLARKVGTTGGITIGLAAAALGLILRAGTGNEWI
                     FLGLSVIALGGMALGNVLVPAWIKTHERGQVLLQTIYGTGLMVGGALGAAFAAPAADA
                     LGGWRPSLRLWGWAILAAIPFWAYLALREHNLPDDHRRPMVAPNLGLLASPTTVALTL
                     MFGIQSMHAYVQFGWLPQIYRDAGLSAGYAGALMSSISGIGLLGALAMPSIIARGHHL
                     RAIFASFGISLIAGYAGLLLAPATLPWVWSTLLALSGFAFPTAIALITARTRTPAVTA
                     QVSGFVQPVGYLLAAIGPVLVGLLHAATGDWRLVLIVLAATGIPLSWASVKLAAPAYV
                     DDELR"
     gene            complement(4215..5102)
                     /locus_tag="PKU75_04995"
     CDS             complement(4215..5102)
                     /locus_tag="PKU75_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPF80267.1"
                     /translation="MTRRHQVGAAGLLVAAGAGAAAGALAGARMISGPRRPWPDYRFS
                     TFEVGVPSEDVTFAADDGTRLSGWWLDRPGSERVVIVSHGHRGNKSDMLGIGPGLWRA
                     GNSVLLFDFRGNGDSDDGPQSLAHYEQADLRAAIEYAVARRPDAALVLVGFSMGASVS
                     ILVAADDPRVRGLVLDSPFADMHGVIANAVTRLRLPAVPLVWLTDRATRWRYGYGFHQ
                     VAPIEAIGRLAPRPILLMHGDQDRVIPLEHAHRLFAAAGEPKHLKVFEGADHCGGYFQ
                     ERAAYIRLVADFVDGIRRA"
     gene            complement(5170..6189)
                     /gene="galE"
                     /locus_tag="PKU75_05000"
     CDS             complement(5170..6189)
                     /gene="galE"
                     /locus_tag="PKU75_05000"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPF80268.1"
                     /translation="MRVLVSGGAGYIGSHTVVQLIAAGHDVVIVDSFANSKPAVIGRL
                     EALTGEAIPVHAFDLTDRDKTERLFADEPFDAVIHFAGLKAVGESAEIPLEYYENNLG
                     TTFSLVRAMRRHGVKMLVFSSSATVYGEEAPLPYREDYAPLQASSPYGRTKVMIEHVL
                     TDVAAADPSLRIGILRYFNPVGAHPSGTIGEDPQGIPNNLMPFIAQVAVGRRDKLVVF
                     GDDYPTADGTCERDYIHVEDLAAGHVAALNTLATSEKAVSVWNLGTGQGTSVLEMVHA
                     FERAVGRELPYEIGPRRAGDQPRSWADPARANDELGWRTVKTIDDMCADTWRWQSSNP
                     TGYED"
     gene            complement(6262..6807)
                     /locus_tag="PKU75_05005"
     CDS             complement(6262..6807)
                     /locus_tag="PKU75_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HPF80269.1"
                     /translation="MKFGHDTHLALGAAVALANSALAPDTLDSIAALDDFYGRWEYTG
                     TRRGTQAELDEVRALRPRLRALLLASEDEAVAMVNSMLAEARALPQLTRHQGFDWHIH
                     AIASDAPLVDRISVETAMAMIDVIRAGELARFGVCADDTCTGIVLDLSRNRSRRFCST
                     ACGNRNAVAAYRARQAERTAG"
     gene            6847..7836
                     /locus_tag="PKU75_05010"
     CDS             6847..7836
                     /locus_tag="PKU75_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPF80270.1"
                     /translation="MPAVTDDVAAVQGASATSSTMGAGLLFAVGGALSFALSGPIARG
                     LLDTGWNAVAVVLIRMGLAALVLLPFGLRALRADWQVLARTWRTITAYGLLAVAGAQF
                     CYFSAVARMDVGPALLIEYTAPAAVVLWLWLRGGQRPGLVTIAGAMVAAVGLVFVLDL
                     LSGGARVDPIGVLWSLGAMIGAAAFFIMSADTDNGLPPLSLAAGGLLVGALALALLAA
                     TGVLPVAYAGASATYAGHEFPWWAPLLALGVVTAATAYVLGIVGTRILGSRLASFVAL
                     SEVVAAVIFAWVLLGQLPSRMQLLGGVLILAGVVLVKLGEPRPLGPELELDVA"
     gene            8264..8590
                     /locus_tag="PKU75_05015"
     CDS             8264..8590
                     /locus_tag="PKU75_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80271.1"
                     /translation="MAFWTIVGLAGLRLLAPTALRTAGPALLLVTIPGAIALATNAWT
                     EPLLFAAFGWWAALMRRGHPNAAMVPLALACATNSTPRSSCSSSPCGRASDCAEASRP
                     APAPWR"
     gene            8598..>8779
                     /locus_tag="PKU75_05020"
     CDS             8598..>8779
                     /locus_tag="PKU75_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80272.1"
                     /translation="MAHRRPRDLIRDTVTYLIDVPPLPYGNSSYLWFLLHRHVTLPTW
                     VTTGALLLALAGAAWVA"
     assembly_gap    8780..8879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8880..9142
                     /locus_tag="PKU75_05025"
     CDS             <8880..9142
                     /locus_tag="PKU75_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80273.1"
                     /translation="WVTTGALLLALAGAAWVARQRHTGLPRILYAAAAVLLVANLVNK
                     QAFFNQYWLVAALVVIALAATPERGPEPDTERDWESAVPGAV"
     gene            complement(9119..9946)
                     /locus_tag="PKU75_05030"
     CDS             complement(9119..9946)
                     /locus_tag="PKU75_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPF80274.1"
                     /translation="MADMPLVHAVNVTKSFGDNQVLKGIDMDVSKGEVVCFLGPSGSG
                     KTTFLRCINQLETIDGGRIWVDGELMGYQDKGGQLYRLHDKEVARQRREIGMVFQRFN
                     LFPHKTVLENIIEAPIQVKKENKAEATARANELLDRVGLSDKPAAYPAQLSGGQQQRV
                     AIARALAMRPKLMLFDEPTSALDPELVGEVLTVMRELADEGMTMIVVTHEMAFARDVA
                     DRVVFMDGGVVVEQGDPRQVISNPQHERTKTFLRRMQTEHEQIVAAVADPLHGPRDS"
     gene            complement(9946..10869)
                     /locus_tag="PKU75_05035"
     CDS             complement(9946..10869)
                     /locus_tag="PKU75_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPF80275.1"
                     /translation="MTTETTDRPGTIDARPVRHPGRWVAVAFIGLLTAMLVNSFVTNE
                     KWDWGYTFEIMQQKPVIEGLIKGTLLGTVLAMVIGVVLGIVLAIMRMSENVVLRGVAF
                     AYTWFFRSIPRYVLLGLFAVGLGYLYNTFEIGIPFTDIRFFTLDYQNYSSTVWVGGLA
                     LGLSEAAYMAEIARAGILSVDNGQGEAAQALGMSPRQTMRRIVLPQAMRVIVPPTGNE
                     TIAMVKDTSLFIAAQIIVELYYQATLFGSRSFKVMPGLMAATLWYLIVCTILMIGQSY
                     LEKRFGRGFGVQAPTKRERKVTALLARTGDH"
     gene            complement(10866..11834)
                     /locus_tag="PKU75_05040"
     CDS             complement(10866..11834)
                     /locus_tag="PKU75_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPF80276.1"
                     /translation="MFRRSPAFALAGLACAALTLTACGSDSLSNTGSSSAASSSDTAS
                     ASASASGSASASGSESAGSGGMDFAAALPEKIKSAGVIKIGTDATYQPNEYLDADGKT
                     VIGMDVELFDAIAAKFGVKTEWIPTGFDSIILGVSSGKYDVGVSSFTVNADRMKEVTM
                     VSYFKAGTQWVTQKGNPKGVDPQNACGMKVAVQKGTVQVDDLDARSKACTDAGKEAIT
                     PLVDPDQAKVTAMVQSGKADAMLVDLPPAIAAVDLTGGAVELLGEQYDSAPYGYVLPK
                     DQTAFGEGIVEALKALEADGTYKQILTKWKTEGGAITDFAVNPSVG"
     gene            12007..12393
                     /locus_tag="PKU75_05045"
     CDS             12007..12393
                     /locus_tag="PKU75_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPF80277.1"
                     /translation="MQNMLIKVGVNALALWIAAAVVSGIHLATGSTSWTSKVVTVVLV
                     AVVFGIVNAVVKPIAKVLSFPAIMLTLGLFTFIVNAFMLQITEWISEALDLSFRIDDF
                     FWDAVMAALVITIVSMVLNFVLPDGE"
     gene            12401..13483
                     /gene="hisC"
                     /locus_tag="PKU75_05050"
     CDS             12401..13483
                     /gene="hisC"
                     /locus_tag="PKU75_05050"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPF80278.1"
                     /translation="MSDAPEPRPLLDDLPAYKPGLPPAPVPGMTAYKISSNENPYPPL
                     PSVLDTVRSAAERMNRYPDASNGALRQALSRFLDVPEDWIACGTGSVAVLGQIITAMC
                     DPDDEVVFAWRSFEAYPIVVTLAAAKGVPVPLTADHRHDLSAMAAAITPRTKVVLVCT
                     PNNPTGTVVTDAELRMFLDRVPRRVLVVIDEAYVEFGADPTAPDAMELLREHPNVCVL
                     RTFSKAYGLAGLRVGYAVAHPPIAVALRMTATPFGVNLLAQEAAVSSLEAGDELDVRV
                     KDLVAERDRVEAALAEQGWRLPPSQANFVWFPLGADTPDFARVCEEQALTVRPYDHDG
                     VRATIGEPEASDRLIEIAATWRAAHT"
     gene            13726..17508
                     /locus_tag="PKU75_05055"
     CDS             13726..17508
                     /locus_tag="PKU75_05055"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HPF80279.1"
                     /translation="MPHVRKTPARRALAALTSAATALLGMAVLAPAAHAAGQASLTAL
                     GTAYTQDFDSLLATGTGTEADLPAGWTFVESSGNTTYSAGTGSSTSGDTYSFGSAGAT
                     ERAFGSLNSSSNSPTVGVVLTNSTGATITSLDVAFTAEQWRHGGATTARTDRLTFGYS
                     TDATGLTGGTWLAAPAMDAASSVTSGTAGPLDGNAAANRNAVSGTVSGLSVPDGGTIV
                     LRWASADVSGSDDGLAIDDLSITPHGTPGGGPTNPSGAGSADPASVVQGGTTTLTVDT
                     TPGTNPDSTGLAVTADLTSIGGSATATLADDGVAPDVTAGDGTFTATATVAASTAPGA
                     KSLPFTVTDAQDRSGTGNIAVTVTALEPCAASDQTIGSVQTSGAAGITGTVTVQGVVV
                     ADYEGASPKLRGFYVQDSGDGDAATSDGIFVFEGDNADRVAVGDVVQVSGTAGENQGQ
                     TQISSTTGIAACGTTGTVPTTEVTLPLTAADDLERYEGMLVSFPQELSVTEHYQLGRF
                     GQVTLSQGGRLQQPTNVVAPGGAANDLQAANNLRRIIVDDDTQAQNPDPIQFGRGGQP
                     LSASNTLRGGDTITGLHGVMTYTWGGNSASPNAYRVRPIGSLGGGAPDFQPDNPRPSA
                     APSVGGSLTVVGMNLLNYFNTFGANACTGGVGGAPTDCRGADNQAEFDRQWRKTVAAI
                     EKMNPDIIGVNEIENDGYGSTSALADLAGHLNAEMGAGTYAYLDVDARTGQVDALGSD
                     AIKVGLLYKPATVTPVGTTAVLNSVAFVNGGDSAPRNRATVAQAFEENATGERLIVNA
                     NHLKSKGSACDAPDAGDGQGNCNQVRVNAVNTLTEWLASDPTETGDTDILLVGDYNSY
                     AMEDPITALQDAGYTHLIKTRLGPDAYSYAFDGQWGYLDHALASESLDSQVTGVADYH
                     INADEPAVLDYNTNFKSANLISSLYAPDEFRVSDHDPVIVGLDLLTPVNEPPVANDQS
                     VTTPEDTPVGITLTGSDPESDPLTYTVTSGPSHGTLSGTAPDLTYTPDPNYNGPDSFT
                     FTVSDGESTSAPATVSINVTAVNDAPRLSILTGGTCLNDTAGRMNLSTGDLDGDAVRV
                     TGTSSNTAVVPNSNIVVSGNGATRTVAVTGKAGKSGRAVITLTASDGTTTVKRSITVW
                     IGNKAANTYTGSNGSDMMLGGGGNDRLTGGGGIDLLCGAGGNDRLTGGEGDDTLNGGD
                     GTDVLIGGGGDDILMGGNGADTMTGNAGADFFDGGPGADRATDFRAAQGDRKVNTP"
     gene            17531..17719
                     /locus_tag="PKU75_05060"
     CDS             17531..17719
                     /locus_tag="PKU75_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80280.1"
                     /translation="MGEIGRPVDFPIWHRGASRRDRARVGTAYRAEELLRQPVISGPM
                     LDGYAARAGRRTPAGCGA"
     gene            complement(17724..18950)
                     /locus_tag="PKU75_05065"
     CDS             complement(17724..18950)
                     /locus_tag="PKU75_05065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPF80281.1"
                     /translation="MGRPLTCRMAGGTVVAIAAGLMATAAPGVAADRASEPTPADLAA
                     RLDQRLPRPALPPITGGQRIPSVQEALDALVAEGAVGTVGAIQRGARHREWASGKKRI
                     WHDAPVGAYAPFRVASNTKTMVATLVMQLVERGEWRLSDRVNDLAPGLLAHHGNITLE
                     QLLSHTSGMPDGVTQALIAHMDEPTWDGFFAALGDPYTDREIITAALAQPWLAAPGTQ
                     FNYSNAGYVVLGQLLQRHTGRSLAALMRERIFVPSGMTKAEFATRPGMPSAALHDAAF
                     TKWRWYSLKNFNPALFSAAGATVASSRDMVKFANALNNGRLVRKATLANMRTPRTPDG
                     YGLGMYRIPDPCRPGRLVYGHDGLSFGTASYALSSADGTRQVVIGLTGRAYVNGVRPI
                     PWDVNTPLLALFAETC"
     gene            19205..19855
                     /locus_tag="PKU75_05070"
     CDS             19205..19855
                     /locus_tag="PKU75_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80282.1"
                     /translation="MPSPASSLPLADAVDLTRTGDIWIFRGTSLADRAIQTMTNAPVN
                     HVGMAVVLDDLPPLMWHAELGKSLVDMWTGDHHRGVQLHDLRAAVEQWQVTYGQRAWL
                     RQLSPAVDRAQEDALLRAIARLDGVAFPNTARLAARWLRGRDAYVSRRERARRGTRPE
                     LAYCAEVVAATMIDMGLLTGDFSLRTYDPGAFWSGDDLPLEPGWSLGDEIAVDPAP"
     gene            20218..>20796
                     /locus_tag="PKU75_05075"
     CDS             20218..>20796
                     /locus_tag="PKU75_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPF80283.1"
                     /translation="MAEQKGPAVGPVIESRDGGPDMVQLVTPEGERVSHPEYDAIADQ
                     VSQEELQGFYRDMVIVRRFDNEATALQRQGELGLWVPCLGQEAAQIGSGRALRRQDFA
                     FPTYREHGVAWCRGVDPLNMLGMFRGVHNGGWDSFEKNFHLYTIIIGAQTLHATGYAM
                     GVLRDDAVGTGDPERDTAVPEVGDDGGVALRVA"
BASE COUNT         3176 a         7381 c         7030 g         3109 t
ORIGIN      
        1 ccttggccgt ctcggtgcgg cgcgggtcga tgaccaccaa acgcccgccg cgcgccttga
       61 gttcgcgcag ccgcccggcg aagtccggca cggtcatgat cgagccattg ctcgccatcg
      121 gattgttgcc gaccaggacg agcaggtcgg tccggtcgat gtccggcacc ggcagcagga
      181 actggtggcc gtagagcagc atggcgatca gctgatgggg gagctggtcg acggacgtgg
      241 cgctgaaggt gttgcgggac ttgatggttc gcacgaggct cacgccatgg gtcagggcgc
      301 cgaggctgtg cacattgggg ttgccctgat agacggcgac ggcgtcgggg ccgtgtgcct
      361 tctgggtcgc gacgatcttt tcggcgacca ggtcgaaggc ctcgtcccag ccgatcggct
      421 cccatgtggc gtcggcgccc ggctccccga cccggcgcac cggcgtgcgc agccggtccg
      481 ggtcggtgtg aatgtcttgc agcgcaatgg ctttcgggca aatgtgcccc cgtgacagcg
      541 gatcggcctc gtggccgcgg atgctgacca cggccgggcc gtcggtcacg acctccaggc
      601 cgcagctggc ttcacacagg acgcaggtga tgtagcgggt ctgcttcgtc gcgtcgctca
      661 cccgcccagt gtgccagcgc ctcggtggcg tgtccgccgc gggcgaacgc ataccgggcc
      721 cacggcaaag gatcggcggg caccatggcg ggcatggacc cgttcgcggc atataccgat
      781 caccggctca cccgccccga cgggcgggtg gtcgcctgga ctcaatgggg tgagccaggc
      841 ggtatgccgt tgctccgggt ccccggcacg cccggctgcc gctgggcggt ccgtgcggac
      901 acgactccgt gggccgaacg cgggctgcgg gtgatcacga ccgaacggcc ggggttcggc
      961 gcctcgaccc cgctgccggg acgcggtttc ctcgagcacg ccgacgatct ggcggccatc
     1021 ctcgaccatc tcggcatcgc ccaggtgcac atgatcggtg gcagcgggtc cgcaccccac
     1081 gagctggcct tcgctcagca atatcccgac cgggtgcggg ccgccacgat ccttgtcggc
     1141 gcagctccct tggaggagaa cgagatcgac agcatgatcc cgctcaacca gcagtcccac
     1201 cgcctgtgcc gggcgggtga tcgcgagggc gaagccgccc tgctcgggcc gatccgcgag
     1261 gccatcctcg ccgaccccct ggccgccttc cgggggatca tggagcacgc gcccgccgac
     1321 gaccaggccg tcatgcgaga cccggactgg caggcggtct tcggccgcgc ccagaccgag
     1381 gcgctcggcg ccaccctgga cggctggctg gatgagtcga tggcgatgag ccagaagtgg
     1441 gaggagatcg tgccggaggc catcacgacg tcgatcacct ggcagcacgc cgccgccgac
     1501 gccaactgcc cccaatcggc cgcccggcgc ctggtcgacc ggctccccaa cggccgcttc
     1561 atcgcctggc ccgacgacgt cggccacctc tacggctacc accacgagcc cgagatcctc
     1621 gacgagttgc tcgcccgcgc ctgacgcccc agcgcaccgg acgacgggtg ggcggcatac
     1681 gacccgccga ccgtcatacc tggcgaggga cggtcagcgg tgcgcgatga tcaggtggcg
     1741 ggtggaatgg gcgacgaagg gttcaccgga gcgcagcctc tcatcgaggg cccgcaactg
     1801 cggttcgtat gcggcggagg tgaaccccgg gacccaccag acgcacttgc gcaggatcca
     1861 gaccacccga agccccagcc ggtcacatcg gccgcatccg cctcggcgac caactcctcg
     1921 tatgaacgca ccagcccact ccatcagggc gcgggccctg cgccggacgc acgacgacgt
     1981 cggccgatgc ccgcccgatg tccgacgtgg tcaaggggcc tatgttgggt ggtatgacgc
     2041 gacgcgaact ccacgtggtg cccgtcccgg gaccgggagc cgaggacgtg ctcatcgggc
     2101 acgacggcac ggcatacacc gggtgcgccg acggcgcgat ctacgcggtg cgtgccgacg
     2161 gcggcgaacc gtggcgggtc ggcgccaccg ggggccggcc actcggcctc gaacggctgc
     2221 ccgagggccg catcctgatc tgcgacgcgc accgcggcct cctcgacatg gccctcgaca
     2281 gcggcgaggt gcgggtcctc gccgaccggg tcgacggcat accgatgcgc ttctgcaaca
     2341 acgccgccgt cagtgacgac ggcacgatct ggttcaccga ctcctcgacg aagtggggca
     2401 tcgaggagtg gaagtcggac ctgatcgacg acacctgcac cggccggctg ctcaaactcc
     2461 tccccggcgg gcagcccgag gtggtgctcg acgggctcag cttcgccaat ggcgttgcgc
     2521 tggcgtcgga tcagtcgttc gtcgtcgtcg ccgagacagg tcaccggcgg ctgcgccgct
     2581 actaccgcac cggtccgctg gccggcaacg ccgacgtctt cgccgcggac ctgcccgctc
     2641 accccgacaa catcgcggga ggctccgacg gcctgctctg ggtcacctac gcctccccgg
     2701 cggacgccac cctcgcccgc ctgcagaagt cgccgaaggc gatccgcacg gttgtccggc
     2761 ggctgccgga gcggctcaag ccgacgccgc agcggacggt gcgggtcgcg gcatacgacg
     2821 acctgggcca ggtggtccac gacgtcgaga ccgacgcaac ccactggcac atggccaccg
     2881 gtgtgcgcga acacgacggc cgggtctggc tgggcagcct tgtcgaaccc gcgctcgcct
     2941 ggttcgactt ggcttgatga cgacgtccgc cggcctatct gtgccggaac gccggactca
     3001 ccgcagttcg tcgtcgacgt aggcgggagc ggcgagtttc accgaggccc aggacagcgg
     3061 tatgccggtg gccgccagca cgatcagcac caaccgccag tcgccggttg ccgcgtgcag
     3121 caggccgacc agcacggggc cgatcgcggc gaggaggtag ccgaccggct gcacgaagcc
     3181 ggacacctgg gccgtgacgg cgggtgtgcg ggtgcgggcg gtgatgagcg cgatcgccgt
     3241 cgggaaggca aagccggaga gggccagcag tgtcgaccac acccagggga gggtggccgg
     3301 ggccaggagc aggccggcat agccggcgat cagcgagatg ccgaaggagg cgaagatcgc
     3361 gcggaggtgg tgtccgcgcg cgatgatgct cggcatcgcc aaagccccga ggagaccgat
     3421 gccggagatg gacgacatga gcgccccggc atacccggcg gacaggcccg cgtcgcggta
     3481 gatctggggg agccagccga actgcacata cgcatgcatc gactggatgc cgaacatcag
     3541 cgtcaatgcg acggtggtgg gggatgcgag cagtccgagg ttcggcgcga ccatcggccg
     3601 gcggtgatcg tcgggcagat tgtgttcccg cagagccaga tacgcccaga acgggatcgc
     3661 tgccaggatc gcccagcccc acaggcgcag cgagggtcgc cacccgccga gcgcgtcggc
     3721 agccggtgcg gcgaaggcgg cgccgagggc tccgccgacc atcagcccgg tgccgtagat
     3781 cgtctgcagc agcacctgcc cgcgctcgtg ggtcttgatc cacgcgggca cgaggacgtt
     3841 gccgagcgcc ataccgccga gcgcgatcac gctgagcccc aggaagatcc actcgttgcc
     3901 ggtgccggcg cgcaggatca gcccgagcgc cgcggcggcc aggccgatgg tgatgccgcc
     3961 ggtcgtcccg accttgcgcg cgagcgttac cgcgaccatg ccgaccagcc cgaagcacag
     4021 tccgggcaac ccggtgaaga cgccggccag cgccgacgac atgtccagcc cggcgcggat
     4081 ctcctcgagc agcggcccgg gcgaggacgc gccgggccgc aggttgagcg agacgagcac
     4141 gatggcgacc aggccgagcg ccagcccgct cggcacccgc cgctcagtca tctcgcggct
     4201 cagtatccga cgggtcaggc acgccggatg ccatcgacga agtcggcgac cagccggatg
     4261 taggccgccc gctcctggaa gtagccgccg cagtgatcgg cgccctcgaa gaccttcagg
     4321 tgcttcggct cgccggcggc ggcgaagagc cggtgcgcgt gctccagcgg tatgacgcgg
     4381 tcctggtccc cgtgcatcag caggatcggc cggggcgcca gcctgccgat cgcctcgatc
     4441 ggcgcgacct ggtggaagcc gtagccgtag cgccagcggg tggcccggtc cgtgagccac
     4501 accagtggca ccgcggggag ccgcaacctc gtgacggcgt tggcgattac cccatgcatg
     4561 tcggcgaacg gcgagtcgag gacgagcccc cgcacgcgcg gatcgtctgc tgccacaagg
     4621 atgctcaccg aggcacccat gctgaacccg acgaggacga gagcggcgtc ggggcggcgg
     4681 gccacggcat actcgatggc ggcccgcagg tcggcctgct cgtagtgcgc cagcgactgc
     4741 gggccgtcgt cggagtcgcc gttgccgcgg aagtcgaaca gcagcaccga gtttccggcc
     4801 cgccacagtc cgggcccgat gccgagcatg tcggacttgt tgccgcggtg accgtggctg
     4861 acgatgacga cgcgctcgct gcccggccgg tcgagccacc acccggacag gcgcgtcccg
     4921 tcgtccgcgg cgaaggtgac gtcctcgctc gggacgccaa cctcgaaggt gctgaagcga
     4981 tagtccggcc aggggcgccg gggcccgctg atcatgcggg cgccggcgag cgccccagca
     5041 gctgcgcccg cgccggccgc cacgagcaga ccggcggccc cgacctgatg gcgacgcgtc
     5101 atagacctca cgacgtttct ttgcttcgcc gcggtgcctc gcgaggaccc cgagagcttc
     5161 ggcatccggc tagtcctcgt atcccgtcgg attggacgac tgccagcgcc aggtgtcggc
     5221 gcacatgtcg tcgatggttt tgactgtgcg ccaacccaat tcgtcattag cacgggcggg
     5281 gtcagcccac gagcgcggct ggtcaccggc gcgccgcgga ccgatctcgt aaggcagctc
     5341 ccggcccacc gcgcgctcga aggcgtgcac catctccagc acgcttgtgc cctggccggt
     5401 gccgagattc cacacggaca ccgccttctc ggatgtggcg agcgtgttga gcgcggccac
     5461 gtggccggcc gcgaggtcct cgacgtggat gtagtcgcgc tcgcaggtgc cgtcggcggt
     5521 ggggtagtcg tcgccgaaga cgacgagctt gtcgcgccgg cccacggcca cctgcgcgat
     5581 gaacggcatg aggttgttgg ggatgccctg ggggtcctcg ccgatcgtgc ccgacggatg
     5641 cgcgccgacg gggttgaaat agcgcaggat gccgatgcgc aggctcgggt ccgcagcggc
     5701 gacatcggtg aggacgtgct cgatcatcac cttggtccgg ccgtagggcg aactcgcctg
     5761 cagcggagcg taatcctcgc gatagggcag cggcgcctcc tcaccgtaga cggtcgcgga
     5821 ggaggagaag acgagcatct tgaccccgtg ccggcgcatc gcacgcacga gcgagaaggt
     5881 cgtgccgagg ttgttctcgt agtactccaa cgggatctcg gcgctctcgc cgacggcttt
     5941 caggccggcg aagtggatga cggcgtcgaa gggctcgtcc gcgaagagcc gctccgtctt
     6001 gtcgcggtcg gtcaggtcga aagcgtgcac cgggatcgcc tcgcccgtga gggcctcgag
     6061 gcgcccgatc accgcgggct tgctgttggc gaagctgtcg acgatcacca cgtcgtggcc
     6121 ggcggcgatg agctggacga ccgtgtggga gccgatgtag ccggcgccgc cggagacgag
     6181 aacgcgcatg aggccacgct atgcgctcgg cccgctgcgg ccgaatccgc cgactgggtc
     6241 agccgcgagc gccccacctt gtcagccggc ggtgcgctcg gcctgacggg cgcgataggc
     6301 cgcgacagcg ttgcggttgc cacaggcggt ggagcagaac cggcgcgacc ggttgcgtga
     6361 caggtcgagc acgatcccgg tgcaggtgtc atcggcacag accccgaacc gggccagttc
     6421 gccggcccgg atgacgtcga tcatggccat ggccgtctcg acgcttatgc ggtcgaccag
     6481 cggtgcatcg ctcgcgatgg cgtgaatgtg ccagtcgaag ccctgatggc gcgtcagctg
     6541 tggcagggcg cgcgcctcgg cgagcatcga gttgaccatg gcgaccgcct cgtcctccga
     6601 ggcgagcagg agcgcgcgca accgcggccg cagcgcgcgc acttcatcca gttcggcctg
     6661 ggtgccccgc cgcgtgccgg tgtactccca gcggccgtag aagtcgtcca acgcggcaat
     6721 ggagtccagc gtgtccggcg ccagcgcaga gttggccagg gcaacggcag cgcccagagc
     6781 caaatgcgtg tcatgaccga atttcacttt gacatattac cgctgacatc ggtaccgtca
     6841 tgcgtcatgc cagccgtgac cgatgacgtg gcggccgtgc agggggcgtc cgccacttca
     6901 tcgaccatgg gtgccggcct gcttttcgcg gtcggcggcg cgctcagctt cgccttgtcg
     6961 ggtccgatcg cccggggcct gctcgacacc ggctggaacg ccgtggctgt cgtgctgatc
     7021 cgcatgggcc tggcggcgct cgtgctcctg cccttcggac tgcgtgcact gcgcgccgac
     7081 tggcaggtgc tcgcccgcac ctggcgcacg atcaccgcat acggcctgct cgccgtcgcc
     7141 ggtgcccagt tctgctattt ctccgcggtc gcgcggatgg atgtcgggcc cgccctgctg
     7201 atcgaataca ccgctccggc cgccgtggtc ctctggctct ggctgcgcgg gggccagcgc
     7261 cccggtctgg tgaccatcgc gggagcgatg gtggccgcag tgggcctggt gttcgtgctc
     7321 gacctcctct ccggcggcgc ccgggtcgac ccgatcgggg tcttgtggtc actcggcgcc
     7381 atgatcggcg ccgccgcctt cttcatcatg tcggccgaca ccgacaacgg cctcccgccg
     7441 ctctcactcg ccgccggcgg cctgctcgtc ggcgcgctcg ccctcgcgct tctggccgcc
     7501 accggcgtcc tccccgtggc ctacgccggc gcgtcggcca cctatgccgg ccacgaattc
     7561 ccttggtggg caccgctgct ggcgctgggc gtcgtcaccg ccgccacggc atacgtgctc
     7621 ggcatcgtcg gcacccgcat cctcggctcg cgcctggcct cgttcgtcgc gctgagcgag
     7681 gtcgtggccg ccgtcatctt cgcctgggtc ctcctcggcc aactcccgag ccgtatgcag
     7741 ttgctcggcg gcgtcctcat cctcgccggc gtcgtcctgg tcaagctggg cgagccgcgc
     7801 cctctcggcc ccgaactgga actcgacgtc gcctgagccg tatcttgtgc gccgggggct
     7861 cgcgcaagaa ggactgagga tcgtggtgcg gagcggcttc tgacgcgact ggtgccgtgg
     7921 gtcttcgtcg gtgcgtggat cgcctacggc gccgccctgc agcgcagtgt ccgcggcaac
     7981 cggccctggg tggtgctcgt cctcaccctc gctgccgcgc tcctggccgg cctcgtcgcc
     8041 gccaacgccg ccgtccgcct gcgccgcatc cacgtcacgg cggcggtcgc cggcaatctg
     8101 gtggcgatgc ttactcgacg gccgcaactt ctatcagatg acctgggccg attcttcgcg
     8161 gcagcggggg atgttcgtcg tcaaggacgc cttcacctag tgcccgtgga ccgtgctgct
     8221 gctcgggccg ggcgaatggc tccctgggga catccgctgg gccatggcct tctggacgat
     8281 cgtcggcctg gccgggctgc gcctgctcgc gccgaccgcc ctgcgcaccg ccggacccgc
     8341 cctgctgctc gtgaccatcc ccggcgccat cgccctggcc acgaatgcct ggaccgaacc
     8401 cctgctcttc gccgccttcg ggtggtgggc cgcgctcatg cgacgcggtc acccgaacgc
     8461 cgcgatggtg ccgctggcct tggcgtgcgc gaccaacagc acgccccgat cgtcttgttc
     8521 gtcctcgccc tgtggccgcg cttcggattg cgccgaggca tcgcgaccgg cgccggcgcc
     8581 ctggcgctga tggcgccgtg gctcatcgac gcccccgcga cctcatccgg gacacggtga
     8641 cctatctgat cgacgtgccc ccactgccat acggcaacag cagctatctg tggttcctcc
     8701 tgcaccggca cgtgaccctc cccacctggg tgaccacggg ggcgctgctg ctcgcgctcg
     8761 ccggcgcggc ctgggtggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     8881 ctgggtgacc acgggggcgc tgctgctcgc gctcgccggc gcggcctggg tggcccggca
     8941 acggcatacc ggcctcccga ggatcctgta tgccgcggcc gccgtcctcc tggtcgcgaa
     9001 cctggtcaac aagcaggcgt tcttcaacca gtactggctg gtcgccgcac tcgtggtcat
     9061 cgcgctcgcc gcgaccccgg agcggggacc cgaaccggac accgaacgcg actgggagtc
     9121 agctgtccct ggggccgtgt agcgggtcgg cgaccgccgc cacgatctgc tcgtgctcgg
     9181 tctgcatgcg gcgcaggaag gtcttggtgc gctcgtgctg cgggttgctg atgacctgcc
     9241 gcgggtcgcc ctgctcgacg acgacgccgc cgtccatgaa gaccacccgg tcggcgacgt
     9301 cgcgggcgaa ggccatctcg tgggtgacga cgatcatcgt catcccctcg tcggcgagtt
     9361 cacgcatgac cgtgaggacc tcgccgacca actcggggtc gagcgctgag gtcggctcgt
     9421 cgaagagcat cagcttgggt cgcatcgcca gggcgcgcgc gatcgcgacc cgctgctgct
     9481 ggccgccgga gagctgcgcg ggatatgccg cgggcttgtc ggagagaccg acgcggtcca
     9541 gcagttcatt ggcccgagcg gtcgcctccg ccttgttctc cttcttgacc tggatcggtg
     9601 cctcgatgat gttctcgagg acggtcttgt gcgggaagag gttgaaccgc tggaaaacca
     9661 tgccgatctc gcggcgctgg cgggcgacct ccttgtcgtg cagccgatag agctggccgc
     9721 ccttgtcctg ataacccatg agctcgccgt cgacccagat gcggccgccg tcgatggtct
     9781 cgagctggtt gatgcatcgc aggaacgtcg tcttgcccga gccggacggg ccgaggaaac
     9841 acaccacctc gcccttgctg acgtccatgt cgatgccctt gagcacctgg ttgtccccga
     9901 aggacttggt cacattgacc gcgtggacca gcggcatgtc cgccatcagt gatcacccgt
     9961 tcgagccagg agtgccgtga ccttacgttc gcgcttggtg ggcgcctgca cgccgaagcc
    10021 acggccgaag cgcttctcga ggtagctctg gccgatcatc aggatcgtgc agacgatgag
    10081 gtaccacaag gtggccgcca tcaggccggg catgaccttg aaagaccgcg acccgaagag
    10141 ggtcgcttgg tagtagagct cgacgatgat ctgcgcggcg atgaacagcg aggtgtcctt
    10201 gaccatcgcg atggtctcgt tgccggtcgg gggcacgatc acccgcatcg cctgcggcag
    10261 gacgatccgg cgcatcgtct gtcggggtga catgccgagc gcctgcgcgg cttccccttg
    10321 gccgttgtcc acggacagga tgcccgcgcg ggcgatctcc gccatgtagg cggcctcgga
    10381 caggccgaga gcgagtccac ccacccagac cgtgctcgag tagttctgat agtcgagagt
    10441 gaagaagcgg atgtcggtga acgggatacc gatctcgaag gtgttgtaga ggtagcccag
    10501 accgacggcg aacaggccga gcagcacata gcgcgggatc gaccggaaga accaggtgta
    10561 cgcgaaggcc accccgcgca gcaccacgtt ctccgacatg cgcatgatgg ccagcacgat
    10621 gccgagaacc acgccgatga ccatggcgag gacggttccg aggagggtgc ccttgatcag
    10681 cccctcgatg accggcttct gctgcatgat ctcgaaggtg tagccccagt cccacttctc
    10741 gttggtgacg aaggagttga ccagcatcgc ggtgagcagg ccgatgaatg cgaccgcgac
    10801 ccaccggccc ggatgccgaa ccggccgggc gtcgatcgtg cccggccggt ccgtggtctc
    10861 ggtcgtcatc cgacgctggg gttaaccgcg aagtcggtga tggcgccgcc ctcggtcttc
    10921 cacttggtga ggatctgctt gtaggtgccg tcggcttcca gggccttgag ggcctcgacg
    10981 atgccctcac cgaacgcggt ctggtccttg gggaggacat agccgtaggg cgcggagtca
    11041 tattgctcgc cgaggagttc gacggctccg ccggtcaggt cgacggcggc gatggccggc
    11101 ggcaggtcga cgagcatcgc atcggccttg ccggactgca ccatcgcggt caccttggcc
    11161 tggtccgggt cgaccagcgg cgtgatggcc tccttgccgg cgtcggtgca cgccttgctg
    11221 cgggcatcga ggtcgtcgac ctgaaccgtg cccttctgga cggcgacctt catgccgcac
    11281 gcattctgcg ggtcgacgcc cttggggttg cccttctgcg tcacccactg ggtgccggcc
    11341 ttgaagtagg agaccatcgt gacctccttc atccggtcgg cgttgaccgt gaaggaggag
    11401 acgcccacgt cgtacttgcc gctgctgacg ccgaggatga tcgagtcgaa gccggtgggg
    11461 atccactcgg tcttgacgcc gaacttggcg gcgatggcgt cgaacaactc gacatccatg
    11521 ccgatgaccg tcttgccgtc cgcgtcgagg tactcgttgg gctgataggt cgcatccgtg
    11581 ccgatcttga tcacgcccgc gctcttgatc ttctcgggca acgcggccgc gaagtccatg
    11641 ccgccggagc cggcgctctc gctgccgctc gcggacgccg aaccactcgc ggacgccgaa
    11701 gcgctcgcgg tgtccgagct acttgcggcg gagctgcttc cggtgttgga caacgaatcg
    11761 gagccgcacg cggtgagcgt cagggcggcg caagcgaggc cggcgagggc gaaagcgggg
    11821 gaacggcgga acacgagatg cctccagtga agcgacgatg cgtggccgcg gggtgcggcc
    11881 ccttcgtggg gagagtagcc cgtccccgaa gtcctgccca gcacgccgac gtcacgatcg
    11941 agtttcccgg cacgaaccgg gcaggtgctg aagccaattg tcagccgggc ctgtcaggat
    12001 gggctcatgc agaacatgct gatcaaagtc ggcgtcaacg ccctcgcgct gtggatcgcg
    12061 gccgccgtcg tcagcggcat ccacctggcc accggctcca cgagctggac gtccaaggtc
    12121 gtcaccgttg tcctggtcgc cgtcgtcttc ggcatcgtca acgccgtcgt caagcccatt
    12181 gccaaggtgc tgtccttccc ggcgatcatg ctgaccctcg gtctgttcac gttcatcgtc
    12241 aacgcgttca tgctgcagat caccgagtgg atctccgagg ccctcgacct gtccttccgg
    12301 atcgacgact tcttctggga cgccgtcatg gccgcattgg tgatcacgat cgtgtcgatg
    12361 gtgctcaact tcgtcctgcc ggacggcgag tgaacgcgcc atgtccgatg cgccagagcc
    12421 ccgcccgctg ctggacgatc tgccggccta caagccggga ctcccgcccg cgccggtgcc
    12481 gggcatgacg gcatacaaga tctcctccaa cgagaacccc taccccccgc tcccgtccgt
    12541 gctcgacacg gtccgctcgg cggccgagcg gatgaaccgc tatcccgacg ccagcaatgg
    12601 cgctctgcgg caagcccttt cgcgtttcct cgatgtcccc gaggactgga tcgcctgcgg
    12661 gacgggcagt gtggccgtgc tcggccagat catcaccgcc atgtgcgatc ccgatgacga
    12721 ggtcgtcttc gcctggcgat ccttcgaggc ctatccgatc gtcgtgaccc tcgccgccgc
    12781 caagggcgtc ccggtgccgc tgacggccga ccaccgtcac gacctgtccg ccatggccgc
    12841 agccatcacc ccccgcacca aggtcgtcct cgtctgcacc cccaacaacc ccacgggcac
    12901 ggtggtcacg gacgccgagt tgcggatgtt cctcgaccgg gtgccgcggc gcgtgctggt
    12961 cgtcatcgac gaggcgtatg tcgagttcgg cgccgacccc accgcccccg atgccatgga
    13021 gttgctgcgc gagcacccca acgtgtgcgt gctgcgcacc ttctccaagg cctacggtct
    13081 ggcgggcctg cgggtcgggt atgccgtggc ccaccccccg atcgccgtcg ccctgcggat
    13141 gacggccact cccttcgggg tcaacttgct cgcgcaagag gcggccgtct cctccctgga
    13201 ggcgggcgac gagttggacg tgcgtgtcaa ggatctcgtc gccgagcgcg atcgggtcga
    13261 ggccgcactg gccgagcagg gctggcgact accgccctcc caggccaact tcgtgtggtt
    13321 ccccctcggc gccgacaccc cggacttcgc ccgggtatgc gaggagcagg cgctgacggt
    13381 gcggccctac gaccatgacg gggtgcgcgc caccatcggc gaaccggagg cctccgaccg
    13441 gctcatcgag atcgccgcca cctggcgggc cgcgcacacc tgaccctgca gacacctccc
    13501 caagggtgag gcgacgaccg gcggccagag cgaagattcg gggaacgccc ggtggttcct
    13561 cggggaacca cggtgtcatt gtccccgaat tccgttgtgt cgcaatcata tttggaagtg
    13621 gcttccgctg atgcgcgtga cgcaggatat cgctgactac cttgacatcg atgcagacga
    13681 cgtgttgatt gggcgcgcaa acaatccgcc tagcaggggt cttccatgcc acatgtccga
    13741 aagacgcccg cgcgccgggc gctcgccgct ctcaccagtg ccgccactgc attgctcggt
    13801 atggccgtgc tcgcccccgc cgctcacgcc gccggccagg cgtcgctgac ggctctcggc
    13861 acggcctaca cgcaggactt cgactcgctg ctcgccacgg gcaccggcac cgaagccgac
    13921 ctgcccgccg ggtggacctt cgtcgaatct tccggcaata cgacctactc cgcaggtacg
    13981 ggatcgagca ccagcggcga cacctattcc ttcggcagcg cgggcgccac ggagcgagcc
    14041 ttcggcagcc tgaacagctc cagcaactcc cccaccgtcg gggtggtgct gaccaactcc
    14101 acgggcgcca cgatcacctc cctcgacgtc gcgttcaccg ccgagcagtg gcggcacggc
    14161 ggggccacca ccgcccgcac cgaccggctc acgttcgggt acagcaccga cgccacgggc
    14221 ctgaccggcg gcacgtggct ggccgcgccc gccatggacg cggcctcgtc ggtcaccagc
    14281 ggcaccgcag gccccttgga cggcaatgct gccgcgaacc ggaatgccgt ctcgggcacc
    14341 gtcagcggcc tgtccgtgcc cgacggtggc acgatcgtcc tgcgctgggc gagcgcagac
    14401 gtctccggct ccgacgacgg actcgcgatc gacgacctgt ccatcacgcc gcacggcacc
    14461 ccgggaggcg ggccgaccaa cccctccggc gccggcagcg ccgaccccgc ctcggtggtt
    14521 cagggcggca cgaccacgct cacggtcgac accacgccgg gcaccaaccc ggacagcacc
    14581 ggactggccg tcaccgcgga cctgacctcg atcggcggct ctgcgaccgc gactctcgcc
    14641 gacgacggcg tcgccccgga tgtcacagcg ggcgacggca ccttcaccgc caccgcgacg
    14701 gtggcggcca gcaccgcacc cggtgcgaag tcgctgccgt tcaccgtcac agatgcccag
    14761 gatcgcagcg gcaccggcaa catcgccgtg acggtgaccg cgctcgagcc gtgcgccgcc
    14821 tccgaccaga ccatcggctc ggtgcagacc tccggagccg ccggcatcac cggaaccgtc
    14881 accgtgcagg gtgtcgtcgt cgcggattat gagggcgcct cgcccaagct gcggggcttc
    14941 tatgtccaag acagcggcga cggggacgcg gcgacctccg acggcatctt cgtcttcgag
    15001 ggggacaacg ccgaccgtgt cgccgtcggc gacgtcgtcc aggtgagcgg caccgccggc
    15061 gagaaccagg gtcagaccca gatctcgtcg acgaccggga tcgccgcgtg cggcacgacg
    15121 ggcacggtcc ccacgactga ggtcacgctg cccctgaccg cggccgacga cctggaacgc
    15181 tacgagggca tgctggtcag cttcccccag gagctgtctg tcaccgagca ctaccagctc
    15241 ggccgcttcg gccaagtcac gctctcccag ggcggccggc tgcagcagcc caccaacgtc
    15301 gtcgcgccgg gcggggccgc gaacgacctg caggccgcga acaatctgcg ccgcatcatc
    15361 gtcgacgacg acacccaggc ccagaacccc gacccgatcc agttcgggcg cgggggccag
    15421 cccctctcgg caagcaacac tctccggggc ggcgacacca tcaccggtct gcatggcgtc
    15481 atgacctaca cctggggcgg caacagcgcc agccccaacg cctatcgcgt gcggcccatc
    15541 ggctcgctcg gcggcggcgc tcccgacttt cagccggaca acccgcgccc gagcgctgcc
    15601 cccagcgtcg gcggttcgct cacggtcgtg ggcatgaacc tgctcaacta cttcaacacc
    15661 ttcggcgcta atgcctgcac cggcggggtc gggggcgcgc ccaccgactg ccgcggggcg
    15721 gacaaccagg ccgagttcga ccggcaatgg cgcaagaccg tcgccgcgat cgagaagatg
    15781 aacccggaca tcatcggggt caacgagatc gagaacgacg gctacggcag cacgagcgcc
    15841 ctcgccgatc tggccggcca cctcaatgcc gagatgggcg ccggcaccta cgcctacctc
    15901 gacgtggacg cccgcacggg tcaggtcgac gcgctgggca gtgacgccat caaggtcggg
    15961 ctgctctaca agccggccac ggtgacgccg gtcgggacca ccgccgtgct caactcggtc
    16021 gccttcgtca acggcggtga ctccgccccg cgcaaccgcg caaccgtcgc gcaggccttc
    16081 gaggagaacg cgaccggcga acggctgatc gtcaacgcca accacctgaa gtcaaagggc
    16141 agcgcctgcg acgctcccga cgccggagac ggacagggca actgcaacca ggtgcgtgtc
    16201 aacgccgtca acaccctcac cgagtggctg gcgagcgacc cgacggaaac cggggacacc
    16261 gacatactgc tggtcggcga ctacaactcc tacgcgatgg aggacccgat cacggcgctt
    16321 caggacgcgg gctacaccca cctgatcaag acgcgcttgg gtccggatgc ctactcctac
    16381 gccttcgacg ggcagtgggg ttacctcgac cacgcgctcg cctccgagag cctcgacagc
    16441 caggtcacag gcgtcgcgga ctaccacatc aacgcggacg agccagccgt gctggactac
    16501 aacaccaact tcaagtcggc gaacctcatc tcctcgctgt atgcgcccga cgagttccgc
    16561 gtctcggacc acgacccggt catcgtcggg ctcgacctgc tcacccccgt caacgaacct
    16621 ccggtcgcga acgaccagag cgtgacgacg cccgaggaca ccccggtggg gatcacgctc
    16681 accggttcgg acccggagag cgatccgctc acctacaccg tcaccagcgg accgagccac
    16741 ggcaccctgt ccggcaccgc gccggacctg acctacaccc ccgacccgaa ctacaacggt
    16801 cccgactcct tcaccttcac ggtgagcgac ggtgagtcca cctcggcgcc cgcgacggtc
    16861 agcatcaacg tcacggcggt caacgacgcc ccgcgcctgt ccatcctcac cgggggaacg
    16921 tgtctcaacg acacggccgg ccggatgaac ctgagtacgg gtgacctgga cggtgacgcg
    16981 gtgcgcgtga ccggaacgtc gtccaacacc gccgtggtcc ccaacagcaa catcgtcgtc
    17041 agcggcaacg gcgccaccag gaccgtggcc gtgaccggaa aggcaggcaa gtccgggcga
    17101 gcggtcatca ccctcaccgc gagcgacggc acgaccacgg tcaagcgctc gatcacggtc
    17161 tggatcggca acaaggccgc caacacctac accggcagca acggcagcga catgatgctc
    17221 ggcggcggcg gcaacgaccg gctcaccggt ggcggcggaa tcgacctgct gtgtggcgcc
    17281 ggcggcaacg accggctcac cggcggcgag ggcgatgaca ccctcaacgg cggcgacggc
    17341 accgacgttc tcatcggtgg tggcggcgac gacatcctca tgggtggcaa cggtgccgac
    17401 accatgaccg gcaacgccgg cgccgacttc ttcgacggcg gccctggcgc cgaccgggcc
    17461 acggacttcc gtgccgcaca gggcgaccgc aaggtcaaca ccccctgagc cggcggccct
    17521 gtctcgtcag gtgggggaaa tcggccgtcc ggtcgatttc cccatctggc accggggagc
    17581 cagccggcgc gatcgcgccc gcgtcgggac ggcataccgt gctgaggagt tgctgcgtca
    17641 acccgtgatc tccggcccca tgctggatgg gtatgccgcc cgcgccggtc gccgcacccc
    17701 ggcggggtgc ggcgcgtgag cggtcagcag gtctcggcga acagcgcgag caggggggtg
    17761 ttgacgtccc acgggatcgg gcgaaccccg ttcacgtagg cgcgtccggt caagccgatc
    17821 acgacctgac gggtgccgtc ggccgagctg agcgcatagc tcgcggtgcc gaacgacagc
    17881 ccgtcgtgcc catacacgag acgaccgggc cggcacgggt ccgggatccg atacatgccg
    17941 agtccatagc catcgggtgt ccgcggcgtg cgcatattgg cgagcgtcgc cttgcgcacc
    18001 aggcggccgt tgttgagggc gttcgcgaac ttgaccatgt cccgggagga ggcgacggtc
    18061 gcgccggcgg cggagaacag agcggggttg aagttcttca gcgaatacca acgccatttc
    18121 gtgaaggcgg catcgtggag ggccgcactc ggcattcccg gccgggtggc gaactcggcc
    18181 ttggtcatcc cggagggcac gaagatccgc tcccgcatca gcgcggccaa ggaccggccg
    18241 gtatgccgct gcagcagctg cccgagcacg acatacccgg cgttggagta gttgaactgc
    18301 gtacccggcg cggccagcca cggctgcgcc agcgccgccg tgatgatctc gcgatccgtg
    18361 tacgggtcgc ccagcgcggc gaagaaaccg tcccaggtcg gttcgtccat gtgcgcgatg
    18421 agcgcctgcg tcaccccgtc cggcatgccc gaggtgtggc tgagcagctg ctccagggtg
    18481 atattgccgt ggtgggcgag cagcccgggc gcgaggtcgt tcacgcggtc gctcaggcgc
    18541 cactcgcccc gctccaccag ctgcatgacg agggtggcga ccatcgtctt ggtgttgctg
    18601 gcgacccgga agggcgcgta agccccgacg ggcgcgtcat gccagatccg cttcttgccg
    18661 gacgcccatt cgcggtggcg tgccccgcgc tggatggcgc cgacggtgcc gaccgccccc
    18721 tccgcgacca gggcgtcgag agcctcctgc accgacggga tcctttggcc gccggtgatc
    18781 ggcggcaggg cgggcctcgg cagacgctgg tcaagccgag cggccaggtc cgccggggtc
    18841 ggctcgctcg cgcggtccgc ggcgacgcct ggcgcagccg tcgccatgag tccggcggcg
    18901 atggcgacga cggtgcctcc ggccatacgg cacgtgagtg gtcgacccat gactgtcccc
    18961 ttcagtagtg gtccggccca gcctgccatg gcccgcggcg aaagtcagcg ggatgtggcc
    19021 tcggcgtagc gctcgcgaag ccggtccctg acctgctccg gcgtatacgc gcgccgcttg
    19081 cgctcctctc gggcgacgac cgcgcccgtc gcaaccacac cggccatacc cgcaagcccg
    19141 atccaccgcc accacctgcg tctgccagcc acggcacgag ggtagttccg caagactgtc
    19201 cggtatgccg tcgcccgcct cctcgttgcc gctcgccgac gccgtggacc tgacccgcac
    19261 gggggacatc tggatcttcc ggggcacatc gctcgccgac cgggccatcc agacgatgac
    19321 gaatgcgccg gtcaaccacg tcgggatggc cgtcgtcctc gacgacctcc ccccgctgat
    19381 gtggcacgcc gaactcggaa agtccctggt ggacatgtgg accggcgatc atcaccgagg
    19441 ggtgcagttg cacgatctgc gggcggcggt cgagcagtgg caggtgacgt atgggcaacg
    19501 cgcttggctg cgccagctca gcccagccgt cgatcgcgcc caggaggatg ccttgttgcg
    19561 ggccattgcg cgcctggacg gggtcgcctt cccgaacacg gcccggctgg ccgcccgctg
    19621 gctgcgcggt cgcgatgcct acgtcagccg ccgcgaacgc gcccgccgcg gcacgcgccc
    19681 cgaactcgcc tactgcgccg aagtcgtcgc cgcgacgatg atcgacatgg gcctgttgac
    19741 cggcgacttc tccctgcgca cctacgaccc cggcgccttc tggtccggag acgatctccc
    19801 cctcgaaccc ggctggtccc tcggcgacga gattgcggtc gacccggcgc cctgatccgt
    19861 ctccgctgtc cccccggcga agcggggcca ggttctccga cgcgggcctg gctataaggc
    19921 ggcccgcgta tgacaacccg gccccgctcc cgcggaaccc ggccccgctc ccgccggtac
    19981 ggacctggac gtgcgaccaa cgcagaaccc gcccggccag gtcggcgcgg cataccggag
    20041 gcgctagcct ggggcgtgaa cgcgcaagcg acaactcgcg gtcacgacga cgtgaccgtg
    20101 ccgcctgcca cgacgagcct cgccaggccg gtcaccccgg cagcgcggcg ttcgcgcgtg
    20161 gcgcattgga cacccgggat cgcggtcatc cccgcagcgg gtggggaacg gagcgagatg
    20221 gctgagcaga aggggccggc cgtggggccg gtcatcgagt ctcgtgacgg tgggccggac
    20281 atggttcaac tcgtcacgcc ggagggcgag cgggtctcgc atccggagta tgacgcgatc
    20341 gcggaccagg tctcccagga ggagctgcag ggcttctatc gcgacatggt gatcgtgcga
    20401 cgcttcgaca acgaggccac cgcgcttcag cgccagggcg aactcggact gtgggtgccc
    20461 tgcctcggac aggaggccgc ccagatcggc tccggccggg ccctgcgccg ccaggacttc
    20521 gctttcccga cctatcgcga gcacggcgtc gcctggtgcc gcggcgtcga cccgctcaac
    20581 atgctcggca tgttccgcgg cgtgcacaac ggcgggtggg actcgttcga gaagaacttc
    20641 cacctctaca cgatcatcat cggcgcccag acgctgcatg cgacgggcta tgcgatgggg
    20701 gtgctgcggg acgacgccgt cggcaccggc gacccggagc gcgacaccgc cgtccccgaa
    20761 gtaggcgatg acggcggtgt cgcgctccgg gtcgcc
//
LOCUS       DAOPJG010000034        20775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2716_length_20775_cov_7.078137, whole genome shotgun sequence.
ACCESSION   DAOPJG010000034 DAOPJG010000000
VERSION     DAOPJG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 20775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20775
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_20775_cov_7.078137"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..748
                     /locus_tag="PKU75_05080"
     CDS             173..748
                     /locus_tag="PKU75_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HPF80284.1"
                     /translation="MFGRKDKTEAPQAAAPVHEERPGAKNRPTPKRRDQEAARKQPLV
                     VADRKAAKKAERAKRMESNTRMRSAMVTGDDRYLPPRDKGPVRRFVRDSVDARWNIAE
                     FLLPLMVMTLALQFFRATWAAYVFLAVYALILIAVLDGFFAWRGIKKKLRAKFPDENL
                     KGLGPYAGMRMMQMRRLRMPKPMVERGEYPS"
     gene            753..959
                     /locus_tag="PKU75_05085"
     CDS             753..959
                     /locus_tag="PKU75_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80285.1"
                     /translation="MDDFRWSIQGRDGQPMSDLASPTFPTQTDAEAWLSETWGELADA
                     GAAAVTLVHFEDVVYGPMSLDPPA"
     gene            complement(989..1456)
                     /locus_tag="PKU75_05090"
     CDS             complement(989..1456)
                     /locus_tag="PKU75_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80286.1"
                     /translation="MTFTARGAFLRGFDHECALSPWGRDGGQVDARILKGEPMTLRPL
                     VNEPAFSAGGVPNITLAVWWPAGADAWHCAPDLDPRYDGGVWLFDVIDGQPETYAEWA
                     RHMYDVDIDADALAKIFRHEELREDDILALNPDADVGAVRHRLDEVAYPVPSA"
     gene            complement(1511..1645)
                     /locus_tag="PKU75_05095"
     CDS             complement(1511..1645)
                     /locus_tag="PKU75_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80287.1"
                     /translation="MNPAVSALAARLPTPATVARWWVSVTASDAILGGEWASFASDPE
                     "
     gene            complement(1642..2751)
                     /gene="gcvT"
                     /locus_tag="PKU75_05100"
     CDS             complement(1642..2751)
                     /gene="gcvT"
                     /locus_tag="PKU75_05100"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPF80288.1"
                     /translation="MSTDGLLTSPLHERHVALGAKMADFGGWLMPIEYPGGGVIAEHT
                     AVRERVGMFDVSHLGKARVSGPGAAAYVNACLTNDLGRIQPGKAQYTLCCNESGGVVD
                     DLIAYLRSDDDVFLIPNAANTATVVDLLQASAPVGISVENLHTAYGVIAVQGTLSDEV
                     LGSLGLPVGHEYMSFVEAEWEGLPVIVCRTGYTGERGYELVPMWENAGPLWDTLAVAI
                     TERGGMPCGLGARDTLRTEMGYPLHGNDLSLDITPVMAGSAWAVGWDKPAFWGKEALT
                     EQRAAKTSRLMRGLLVAGRGIPRSHCVVKDASGAEVGEVTSGTFSPTLKQGIALAQLE
                     RSVAMGDTVTIDVRGRGLEAQVVKPPFVDVQTKEA"
     gene            2836..4377
                     /locus_tag="PKU75_05105"
     CDS             2836..4377
                     /locus_tag="PKU75_05105"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPF80289.1"
                     /translation="MTILTLTDQPVAAHKADALVLATQPDGDGATLAPGHGLQRAAAT
                     HLKGALAAFGATGRVDDVHTVAMVPGVAARKVVLTGIAPKSGDGKPTADTLRLGTGAA
                     LRAAGTADTVIVDVPAGTEALLAAAAEGAYLGTYAFTAHKTTGTSAKGGRKTKSATGN
                     VGAIRIQVADAKDRAAKATLKRAETLSAATAYARDLVNTPANILYPQTFADSVAARVA
                     ERKLPVEITVHDEKKLASLGCGGIVGVGQGSARPPRIVELHYTPKRPAGRIAFVGKGI
                     TFDTGGVCIKPAASMLTMKCDMAGAAAVAAAVMAAAEIGLPVAVSGYLCLAENMPGGN
                     AQRPGDVVTMRSGTTVEIIDTDAEGRMVLADGISLAVESGADAVVDVATLTGACMVAL
                     GTRVAGVMANDEPLSRRVLASAARAGEPMWPLPLPEELRAKLDSSVADLQHKGDMYGG
                     ALTAGLFLREFAKDAEGTAIPWAHIDIAGPAFQTEAPFGHVGKGGTGYAVATLLDLAA
                     SWGAK"
     gene            4374..5744
                     /gene="lpdA"
                     /locus_tag="PKU75_05110"
     CDS             4374..5744
                     /gene="lpdA"
                     /locus_tag="PKU75_05110"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPF80290.1"
                     /translation="MSEPDEADVLILGAGSGGYACALRAAQLGLRVTLVEKDKVGGTC
                     LHAGCIPTKAMLHAAEIADTARHSASVGVDLGEPRIDMGRVAAYRQGVVDRLFKGLTG
                     LIAGRGIDVVAGEGRVVDAHTVAVGDRMLRGRHLVLATGSVAKTIPSIPLSDKVITSD
                     GALALTAVPRRVAVLGGGVIGVEFASVLRSFGAEVTIVEALPRLVAAEDEALSAGLQR
                     AFRQRGIATRLGASVSGVSESGDGIRLEVDGDEPVEADLLLVAVGRGPAPVPGIENLA
                     VETDRGYVPVDERCRTTVEGVYAVGDLVPGLQLAHRGFAQGILVAEVLAGLGPAPIVE
                     SGIPRVTYCDPEVASVGLTEAAAREKYGEVETVEYNLAGNGKSQILQTKGFVKLVRAK
                     DGPVVGVHMLGARVGEQIGEAQLIVGWQAHPEDVAPLVHAHPTQNEALGEAHLALAGK
                     PLHAHA"
     gene            5828..>6283
                     /locus_tag="PKU75_05115"
     CDS             5828..>6283
                     /locus_tag="PKU75_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HPF80291.1"
                     /translation="MSERVTMPALGESVTEGTVTRWLKSVGDQVAVDEPLLEVSTDKV
                     DTEIPSPVAGVLLEILAEEDETVAVGADLAVVGDASEGGGSAQEAPAAQQEPAAQEAP
                     APQEAPAAREAPAAQPAPQAAPQQEAPQQQAPADQQAPAEPSGGGSVEGG"
     assembly_gap    6284..6383
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6451..7914
                     /gene="sucB"
                     /locus_tag="PKU75_05120"
     CDS             6451..7914
                     /gene="sucB"
                     /locus_tag="PKU75_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HPF80292.1"
                     /translation="MPALGESVTEGTVTRWLKAEGDDVAVDEPLLEVSTDKVDTEIPS
                     PAAGKLTKILAQEDETVAVGADLAVIGGTVSGGGDAPAAAAQQAAPAEQAAPAAEPEA
                     PAAVEDAATAQPEPSTEAEAPKGESEAPKDEAAPGEPAPQEAAPQQAALQEATPAAPA
                     PAAPAASGAAAAAGDAAAYVTPLVRKLAAENDIDLASVTGTGIGGRIRKQDILDAAEK
                     AKAAVAPATPQPAAQATAPAAAPDAAPAVADSRRGTREKMSRLRKIIAQRMVESLQVS
                     AQLTTVIEVDMTKVARLRDRAKGEFEKREGTKLSFLPFIALASVEALKTHPGVNASIE
                     GEEIVYHGSENLAIAVDTPKGLIVPVIKNAGDLNIAGLARSIADVAARTRDNKITPDE
                     LSGGTFTITNTGSRGALFDTPILNQPQVAMLGSGAIVRRPVVVEVDGAETIAIRSMMF
                     LALSYDHRIVDGADAARFLTTVKERLEEGRFEASLGL"
     gene            8017..8946
                     /locus_tag="PKU75_05125"
     CDS             8017..8946
                     /locus_tag="PKU75_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPF80293.1"
                     /translation="MQQGSARPLTVAVTGASGLIGRALVAALRERGDEVITLVRREPV
                     SAGERRWDPATGHLDPQALAGVDAVVNLAGPGVGDKRWTPAYKARVLRDRVSATEAVA
                     EAVAARLASDDRAIHLVNASAVGYYGDRGEELLTEDSPGGLGFLAEVCRAWEGATQAA
                     SAAGAPVTLARTGLVMSGAGGALAPLVRATRLGAGGPMGSGRQFWPVISRRDEIAALL
                     FLLDHPDLTGPVNLVGVEPPRQREVAGALGRLLHRPAILPAPGAALRAVIGEFAGEIM
                     ASQRVLPDRLLAAGFVHQDRALTAVLASALAEE"
     gene            8968..10314
                     /locus_tag="PKU75_05130"
     CDS             8968..10314
                     /locus_tag="PKU75_05130"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPF80294.1"
                     /translation="MSHPTCSRSHAARRLALLGAAVIPVSIAAGAAGASGATGPTVTL
                     TTPTAGSTNTDGRVTVTGTYSGPNKVTSVTVSGCRPDGSRCKDYLLANTNGAFSGKWR
                     GFPARLTPTSSDGKSGTFTVVLSGLPGTRLRISAFASDAVTPRGGSATVNVAVKAPED
                     PAFISVVFGRASWKAASGEGCTNQPNTARTLEQDAKDMALLGLMGVAQTVTSRVDETE
                     RLCPDNFAVEASWVDLARMRDAYGWSLTSQSKTYPDLTKLTTTQVIDETAGTLPIFQA
                     HGHTRAWGSFAYPNNKQNSTVQRIATNYFAFGRKYANDLNSRQNSMVFPYEMRTLSVN
                     GGRCINVNLPCSTMPMVNDRVTTSPNNIGRILKPGPGHWGVVQFYRIVEGVEGKLGQS
                     FAWDCTSSDWRNRWTSQPELYCRNTMIEGLEKRNKSATSVDPAAVAIAWGRKPFGS"
     gene            complement(10408..>11180)
                     /locus_tag="PKU75_05135"
     CDS             complement(10408..>11180)
                     /locus_tag="PKU75_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80295.1"
                     /translation="RQTAPDNDPFGLAHDEGLGERALAAWDWLCASPIRYLAASLAVL
                     LVSAGAVLALRPDNPQTLAPAPFAASSATTQSGPGPSEREPVPSASPQSTPAQSTPAQ
                     SPTARTTPDPGSRTAAPADPVRARSGALDEPKAVVQALADLRAQAWTDLDARALVRLN
                     AGGSPAADADDAALARARRDDLAYEGLRFTVRSATARAIDGADRRVRVRAVLDASEFT
                     ITDANGRTTTVPAAPHTQIVFDLAWSGSRWQVRDLREA"
     assembly_gap    11181..11280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11281..12205)
                     /locus_tag="PKU75_05140"
     CDS             complement(<11281..12205)
                     /locus_tag="PKU75_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80296.1"
                     /translation="MDVTLTGYRLRPATTAPDGSTRWIGTRESDGTRVAVLVRPGEED
                     DRAGYAGLHLPGVLTLAEVHAVPGGCALLYADPPAHTLEDLIADGPICASGVVEVLLT
                     LHAALAGLAGRGQGHPVLDAGGVWVDADGSVLLGDVGGWPADPDPVAAQRCLLQLGTT
                     LLADVAPLDAGVARLGLDAVEEVRRELADALVRWQHQVETSGQPLDDLEQRLRAVCEP
                     APPRIPVEWGRQASDLAGMLRALARADDPDPGSRSRFSSARFTIRSPRAGATTTAGEG
                     PPVHSATAAPARRGRRAGPPARGRLALRRTGG"
     gene            complement(12301..13179)
                     /locus_tag="PKU75_05145"
     CDS             complement(12301..13179)
                     /locus_tag="PKU75_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPF80297.1"
                     /translation="MTRPIGVGVCILPDRPWAQARQDWLSADELGFDHAWTYDHLTWG
                     GLPDSPWFAAVPTLAAAALETRRIRLGTFVSSPNTHHPAQLVRESLSLHDLSGGRLLL
                     GVGAGGDTDARLTSPHASAGARSARFREFVTVVDRMLREDGISHHGPCYDLDGAGSLP
                     GPPNAGEQHGIPLLVAANGPRAIALAAERGDGWITYGGKADDDAAWWALVAGAAGRMD
                     AEVSRAGRNRPLRRYLGLDSQPTYPFSSADCFEDAAGRAAALGFTDVVCSWPRPEPPY
                     AGDIRVVEEVASRRRA"
     gene            complement(13176..15401)
                     /locus_tag="PKU75_05150"
     CDS             complement(13176..15401)
                     /locus_tag="PKU75_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPF80298.1"
                     /translation="MSGRVGSGGAHAIPQQRRTRRPRLVAALVVLLWLAVSAVGGPLV
                     GRLAEVQKNDNASFVPATAESTEVADFVQKASANNDLPYLLVLESPTAITPAQQAAAA
                     ALVQKLPAAPLDKLGPGRTIGEFIGSPTSAPIPSQDGKALLVVLNLDNAKAEDTINKT
                     TPLYETALELRTLLDTDLKGTGLTGHVAGPGGLLSDFVTAFEGIDGVLLLVALVVVLL
                     ILLVVYRSPILPFAVLISAVFGLALAALAVFPLAKAGAIDLSGQSQGIMFILVVGAAT
                     DYALLLVSRFREALHDTDDTWDALRSAWRGAVEPILASGATVILGLLCLMLSDLGSTA
                     GLGPVGALGIAGAMAASLTFLPAVLLLVGRRIFWPVAPKLDHRHLEDALETRGVWGRV
                     AKLVGTHPRRTWVITLAGLLAFAAFIPTFKADGLSADELFLTEVPSVEAGEALARHYP
                     AGAGSPVQIVVPQPQAEAVVERLRTEADVQDPYVGLVPGAPPVAVDGKHIVQATLAVP
                     ADSPEATEVVRRLRADLDAVTPDALVGGSTAVSLDVLDATERDLRVIIPAILIVIFIV
                     LALLLRSLVAPVLLVVANVVSFAATVGVAALVFNHILDFPNSDPATPLYGFVFLVALG
                     IDYSIFLMTRVREEAQRRGTRPGILTGLAVTGGVITSAGIVLAATFSALAVLPLIFLV
                     QIGFIVAFGVLLDTLIVRSLLVPALAYDIGPRIWAPSRLAREDAAVSASTGEAAHPDR
                     S"
     gene            complement(15398..16183)
                     /locus_tag="PKU75_05155"
     CDS             complement(15398..16183)
                     /locus_tag="PKU75_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPF80299.1"
                     /translation="MAVATINCFSDVLEVGVTFTVVLPQVTQAQVGVESVERVGDPPV
                     LYLLHGLSDDHSAWTRYTSVERYADARGLAVVMPAVSRSFYADEAGGHRYWTFVSEEL
                     PQLVRTFFRVSTDPAQTFVAGLSMGGYGALRLALTYPERYAAVASLSGAVDVRALMRE
                     PGRARLNEVIFAGEVPPEADLFSLVRSGRSLPPMWIGCGTEDILYPMVRDFEAAAQGV
                     GADVTTLYEPGDHVWELWDAQLPVVLDFLLSHGDSARSSVAAR"
     gene            16284..16922
                     /gene="lipB"
                     /locus_tag="PKU75_05160"
     CDS             16284..16922
                     /gene="lipB"
                     /locus_tag="PKU75_05160"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPF80300.1"
                     /translation="MRFEHLGFDPDFVDYLAAWDHQREVHAQVVDGSLPDTVLLLEHA
                     AVFTAGKRTLPLERPFDGTPVIDVDRGGKITWHGPGQLVGYPIVRLPNPVDVVAHVRR
                     LEEVMIRTCADFGVEVTRVDGRSGVWIVGTGIGDPDRKLGAIGVRVSQNVTMHGFALN
                     ANPDLGWASVIVPCGIADAGVSSLSIEAGREITVADVLPYTEKHLAEVLTLS"
     gene            complement(17255..17872)
                     /locus_tag="PKU75_05165"
     CDS             complement(17255..17872)
                     /locus_tag="PKU75_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80301.1"
                     /translation="MARKSTIITSAVGAAAGLVLAGGVVLAANADDTTTTAPSTSTSQ
                     SANTDSGLPGLNQQGTTDQGTTDLGASGQQGQMGPGGHGGQPGDGQMGGSQDTAVTGD
                     EADKVVAAVQAKDSAASITEVRKDPDGSYDALGTKDGSPVFFEVSADLATITENTGGG
                     MGGHGGQLGGQPGQAPDGTSQQGSSLDPNTSGSATSDSTAANGTT"
     gene            18090..19112
                     /gene="lipA"
                     /locus_tag="PKU75_05170"
     CDS             18090..19112
                     /gene="lipA"
                     /locus_tag="PKU75_05170"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPF80302.1"
                     /translation="MTIAPEGRKLLRVEARNAETPIERKPSWIRTTAKMGPQFTEINA
                     LVKGAGTHTVCQEAGCPNIFECWEDREATFLIGGDTCTRRCDFCDIATGKPQPLDRDE
                     PRRVAESVATMGLRYSTVTGVARDDQPDGAAWLYAETVRQIHALNPNTGVEILPPDFG
                     AQPELVGQVFDARPEVFAHNLETVPRIFKRIRPAFTYEKSLRVLTMAREADLVTKSNL
                     ILGMGEQEAEIAQAIEDLYAAGCDILTITQYLRPSPRHHPIDRWVKPEEFVHWSGYAE
                     GLGFKGVMAGPLVRSSYRAGRLWASAMTKSGRGIPPHLAHLGEAGEPARQEAATLLAG
                     GAKAGA"
     gene            19132..19851
                     /locus_tag="PKU75_05175"
     CDS             19132..19851
                     /locus_tag="PKU75_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HPF80303.1"
                     /translation="MAKDPAAPKKQRSNPFKQIASVYKAVKSMDPSITWWLLGVFIGV
                     LALFVIIGFLVDHPIYFVFIGLPIAALAAMITLSRRGEKAAFGAMQGKAGQSVGALSM
                     LRRGWYYDQEPVAADVARPQEIANAAMIFRALGAPGVVLIGEGPLPRAKKLMEKEVKK
                     VARVAPGVPIHQLYVGDDDGQVSARKLTRTMTKLKGTLTKEEMSAVNKRLKSIPGIRQ
                     GIPAGVDPTKARMDRRQLRGR"
     gene            20040..>20775
                     /locus_tag="PKU75_05180"
     CDS             20040..>20775
                     /locus_tag="PKU75_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HPF80304.1"
                     /translation="MFSSADEVLAFIKDQDVKFVDIRFCDLPGVMQHFNVPAESLTED
                     FFTEGQAFDGSSIRGFQAIHESDMKLVPDVATAYLDPFRAEKTLIMNFSIVDPFTDQP
                     YSRDPRNIAIKAEAYLKSTGIADTAYFGAEAEFFVFDDVRFETKPQAGYYFIDSVEAA
                     WNTGREEEGGNRGYKTRVKGGYFPVPPVDHFGDLRDKICLNMKEVGLTVERSHHEVGS
                     AGQQEINYKFNTLLHSGDDMMKFKYVV"
BASE COUNT         3191 a         7311 c         7219 g         2854 t
ORIGIN      
        1 ggcctggtcg aaggcgtcga gcgagacgct cgggatgcgg gtgagggcct cgaggtcgcg
       61 gcggatgtcg ggcatcagct cggaaagtcg ggcgcgcagc tggtcgagat cgatgacgtc
      121 ggtgctcacg cgtcgacact atccgtctcg tcgcgggccg tatccttgcc gggtgttcgg
      181 acgcaaggac aagaccgagg ccccgcaggc tgccgccccg gtccatgagg aacgccccgg
      241 ggccaagaac cggcccacgc ccaagcggcg cgatcaggag gcggcgcgca agcagccgct
      301 cgtcgtcgcc gatcgcaagg ccgccaagaa ggccgagcgc gccaagcgta tggagtccaa
      361 cacccggatg cgatcggcca tggtcaccgg cgacgaccgc tacctgccgc cgcgcgacaa
      421 gggtccggtg cgccggttcg tgcgcgacag cgtcgacgcc cgctggaata tcgccgagtt
      481 cctgctgccg ctgatggtca tgacgctggc gctgcagttc ttccgcgcca cctgggccgc
      541 ctatgtcttc ctcgcggtct atgcgctgat cctcatcgcc gttctcgacg gtttcttcgc
      601 ctggcggggc atcaagaaga agctgcgggc caagttcccg gacgagaacc tcaagggcct
      661 cggcccgtat gcgggtatgc ggatgatgca gatgcgtcgc ctccgcatgc ccaagccgat
      721 ggtcgagcgc ggcgaatacc cgagctagcg ccatggacga cttccgctgg agcatccagg
      781 gccgcgacgg gcagccgatg tccgacctgg cctcccccac cttccccacg cagaccgacg
      841 ccgaggcctg gctctcggag acctggggcg aactcgccga cgccggcgcg gcggcggtga
      901 ccctggtgca cttcgaggac gtcgtctacg ggccgatgtc cctcgacccg cccgcctgac
      961 cccacctgac cctgcccgtg ggcggggatt acgccgacgg caccgggtag gcgacctcgt
     1021 cgagccggtg ccgcactgcg ccgacatcgg cgtcggggtt gagcgcgagg atgtcgtcct
     1081 cgcgcagttc ctcgtgccgg aagatcttcg ccagggcgtc ggcgtcgatg tcgacgtcgt
     1141 acatatggcg tgcccattcg gcgtaggtct cgggctgccc gtcgatcacg tcgaagagcc
     1201 agacgccgcc gtcatagcgg ggatccaagt ctggcgcgca gtgccaggcg tccgctccag
     1261 cgggccacca cacggccagc gtgatgttgg ggaccccacc cgcgctgaag gccggctcgt
     1321 tgacgagcgg ccgcagcgtc atcggctcgc ccttgagaat ccgggcgtcg acctgacccc
     1381 cgtcgcggcc ccacggcgac aacgcgcact catggtcgaa gccgcgcaag aacgcaccgc
     1441 gggccgtgaa ggtcaccgag gcgtcgtcac cactgccgct gcgatacgtg gcacagtgct
     1501 cgttctcgcc tcactccggg tcggacgcga agctcgccca ctcgccaccg aggatcgcgt
     1561 cggaggccgt gaccgacacc caccagcggg cgacagtggc gggggtgggc agtcgcgcgg
     1621 cgagcgcgct gaccgccggg ttcatgcttc cttggtctgc acgtcgacga atggcggctt
     1681 gacgacctgg gcctcgaggc cgcggccgcg cacgtcgatc gtgacggtgt cgcccatggc
     1741 caccgagcgc tccagctgcg ccagcgcgat gccctgcttc agcgtcggcg agaaggtccc
     1801 cgaggtcacc tcccccacct cggcgcccga cgcgtccttg acgacgcagt gcgagcgcgg
     1861 gatcccgcgg cccgccacca ggaggccgcg catcagccgc gaggtcttgg ctgcccgctg
     1921 ctcggtcagc gcctccttgc cccagaacgc cggcttgtcc cagccgaccg cccacgcgga
     1981 cccggccatg accggggtga tgtcgagcga aagatcgttg ccgtgcaacg gatatcccat
     2041 ctcggtgcgg agggtgtccc gggcgccgag accacacggc ataccgcccc gctcggtgat
     2101 cgcgacggcg agggtgtccc acagcggccc ggcgttctcc cacatcggca ccagttcgta
     2161 gccgcgctcg ccggtgtatc cggtgcgaca gacgatgacc ggcagcccct cccactcggc
     2221 ttcgacgaag gacatgtact cgtgcccgac gggcaggccg agggagccga gtacctcgtc
     2281 ggagagggtg ccctggacgg cgatgacacc gtatgccgtg tgcaggttct cgaccgagat
     2341 ccccacgggc gcgctcgcct gcagcaggtc gacgaccgtg gccgtgttgg cggcgttggg
     2401 gatgaggaag acgtcgtcgt cgctgcgcag gtaggcgatc aggtcgtcga cgacaccgcc
     2461 ggattcgttg cagcacaacg tatattgcgc cttgcccggc tggatgcggc cgagatcgtt
     2521 ggtcaggcag gcgttgacat atgccgccgc tcccggcccg ctcacgcgcg ccttgccgag
     2581 gtgcgagacg tcgaacatcc ccacccgctc gcgcaccgcg gtgtgttcgg cgatgacgcc
     2641 gccgccggga tattcgatcg gcatcagcca gccgccgaag tcggccatct tggcgccgag
     2701 tgctacgtgg cgctcatgca ggggcgaggt cagcaagccg tcggtgctca tggcacgaag
     2761 gtagcgcgtc ggctcacccg cggtgcgctc gaagcccttc cccccaaccg cgatacccac
     2821 cggtagattg ccctggtgac catcctgacc ttgaccgacc agcccgttgc tgcccacaag
     2881 gccgacgccc tggtacttgc cacccaaccc gacggcgatg gggccacgct ggcccccgga
     2941 catgggctgc agcgcgccgc cgccacccat ctcaagggcg ccctcgccgc cttcggcgcg
     3001 accggccggg tcgacgacgt gcacacagtg gcgatggtgc cgggcgtggc cgcccgcaag
     3061 gtcgtgctga ccggcatcgc acccaagtcc ggcgacggca agccgaccgc ggacacgctg
     3121 cgactgggca ccggtgccgc gctgcgtgcc gccggcacgg ccgacaccgt gatcgtcgat
     3181 gtccccgccg gcaccgaagc actgctcgcg gctgcggccg agggcgccta cctcggcacc
     3241 tacgcgttca cggcgcacaa gacgacgggg acgtcggcca agggcggccg gaagaccaag
     3301 tccgccaccg gcaacgtcgg ggcgatccgc atccaggtcg ccgacgccaa ggaccgcgcc
     3361 gccaaggcga ccctcaagcg cgccgaaacg ctcagtgcgg caacggcata cgcccgcgac
     3421 ctggtgaaca cccccgccaa catcctctat ccgcagacct tcgccgactc cgtcgccgcc
     3481 cgggtcgccg agcgcaagct gcccgtcgag atcaccgtcc acgacgagaa gaagctcgcc
     3541 agcctcggct gcggcggcat cgtcggcgtc ggccaggggt cggcccgacc gccacggatc
     3601 gtcgaactgc actacacccc caagcgccct gccggacgga tcgcgttcgt gggcaaggga
     3661 atcaccttcg acaccggcgg tgtgtgcatc aaacccgccg cctcgatgct gacgatgaag
     3721 tgcgacatgg ccggtgctgc ggcggtcgcc gccgcggtca tggccgccgc cgagatcggt
     3781 ctgccggtgg ccgtcagcgg ttacctgtgc ctcgccgaga atatgccggg cggcaacgcc
     3841 cagcgccccg gcgacgtcgt cacgatgcgc tcgggcacca cagtcgagat catcgacacc
     3901 gacgccgagg gccgcatggt cctcgccgac gggatctcgc tggccgtcga gtccggcgcg
     3961 gacgccgtcg tcgacgtcgc caccctcacc ggtgcctgca tggtggccct cggcacgcgg
     4021 gtcgcgggcg tgatggccaa cgacgagccg ctctcgcggc gggtgctcgc cagcgccgcc
     4081 cgggccgggg agccgatgtg gccgctgccg ctgcccgagg agttgcgcgc caagctcgac
     4141 tcgagcgtcg ccgacctgca gcacaagggc gatatgtacg gcggcgccct gaccgccggc
     4201 ctgttcctgc gcgagttcgc caaggatgcc gagggcacgg cgatcccgtg ggcgcacatc
     4261 gacatcgccg ggccggcctt tcagaccgag gcgccgttcg gccacgtcgg caagggcggc
     4321 acggggtatg ccgttgccac cctcctcgac ctcgccgcct cctggggcgc gaagtgagtg
     4381 agcctgacga ggccgatgtc ctgatcctcg gggcgggtag cggcggctat gcgtgcgccc
     4441 tgcgcgccgc gcaactcggc ctgcgcgtga ccctcgtcga aaaggacaag gtcggcggca
     4501 cctgtctgca cgccggctgc atcccgacga aggcgatgct gcacgccgcg gagatcgccg
     4561 acaccgcccg gcacagcgcg tcggtcgggg tcgacctcgg cgagccgcgg atcgacatgg
     4621 ggcgggtcgc ggcataccgc caaggcgtcg tggaccggct cttcaagggc ctgacgggcc
     4681 tgatcgcggg acgcggcatc gacgtcgtcg cgggtgaggg ccgcgtcgtc gacgcccaca
     4741 cggtcgcggt gggggaccgt atgctgcgcg gtcgccacct cgtgctcgcc accgggtcgg
     4801 tggccaagac aatcccgtcg atcccgttgt cggacaaggt gatcaccagc gatggggcgc
     4861 tggcactgac ggccgttccg cgacgggtgg ccgtgctcgg cggcggggtc atcggggtcg
     4921 agttcgcgtc ggtcctccgt tccttcggcg ccgaggtgac catcgtcgag gccctccccc
     4981 ggttggtcgc tgccgaggac gaggccctct cggcgggcct gcagcgggcc ttccggcaac
     5041 gcggcatcgc aacccggctc ggcgccagcg tgagcggcgt gagcgagtcc ggcgacggca
     5101 tacgactcga ggtggacggc gacgagccgg tcgaggccga cctgctcctc gtggccgtcg
     5161 ggcgtggccc ggcgccggtg cccggcatcg agaacctcgc ggtcgagacc gatcgcggct
     5221 acgtccccgt cgacgagcgg tgccgcacca cggtcgaggg cgtgtatgcc gtcggcgacc
     5281 tcgtccccgg cctgcagctg gcgcatcgcg gcttcgccca gggcatcctc gtcgccgagg
     5341 tcctcgccgg gctgggcccg gcgccgatcg tcgagagcgg catcccgcgc gtgacctact
     5401 gcgacccgga ggtggcgtcg gtcggtctga cggaggcggc ggcgcgcgag aagtatggcg
     5461 aggtcgagac cgtcgaatac aacttggccg gaaacggcaa gagccagatc ctgcagacca
     5521 agggcttcgt caagctcgtg cgcgcgaagg acggcccggt cgtcggcgtg cacatgctcg
     5581 gggcgcgcgt cggcgagcag atcggcgagg cccagctgat cgtcggctgg caggcccacc
     5641 cggaggatgt cgcgccgctc gtccatgccc acccgaccca gaacgaggcc ctcggcgagg
     5701 cccatctcgc gttggccggc aaaccgctgc acgctcatgc gtgaacgccg gtggcatatc
     5761 cgccccgaat gcgtgagagt gtggtctgca caacatctca atcgagcccc aaggagatcg
     5821 aggactcatg tctgaacgcg tgaccatgcc cgcacttggc gaatccgtca ccgaaggcac
     5881 cgtgacgcgc tggctgaagt cggtcggtga tcaggtcgcg gtcgacgagc cgctgctgga
     5941 ggtctccacc gacaaggtcg acaccgagat cccgtccccg gtcgccgggg tgctgctcga
     6001 gatcctcgcc gaggaggacg agacggtcgc tgtgggcgcc gatctcgccg tggtcggcga
     6061 tgcctccgag ggcggcgggt ccgcccagga ggcacccgct gcccagcagg agccggcggc
     6121 ccaagaagcc cctgctcccc aggaagcgcc ggccgctcgg gaagcgcccg cagcgcagcc
     6181 cgcgccccag gcagcaccgc agcaagaggc tccgcaacag caagcccctg ccgatcaaca
     6241 agcccccgcc gaaccgtccg gcggcgggtc cgtcgagggc ggcnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnntgccgat caacaagccc ccgccgaacc gtccggcggc
     6421 gggtccgtcg agggcggcac gaccgtgacg atgccggcgt tgggcgagtc cgtcaccgag
     6481 ggcaccgtca cccgctggct gaaggctgag ggcgacgacg tcgccgtcga cgagccgctg
     6541 ctggaggtct ccaccgacaa ggtcgacacc gagatcccct ccccggccgc cggcaaactg
     6601 accaagatcc tcgctcagga ggacgagacc gtcgcggtcg gcgccgacct ggccgtcatc
     6661 ggcggcaccg tctcgggtgg cggtgacgcc ccggccgccg ctgcgcagca ggctgccccc
     6721 gcagagcagg ccgcccccgc cgcggagccg gaggcgcccg ccgccgtcga ggatgccgcg
     6781 acggcccagc ccgagccctc gaccgaggcc gaggcaccca agggcgaatc ggaagctccc
     6841 aaggacgaag ccgcccctgg cgagcccgcc cctcaggaag ccgcgcccca gcaggccgcc
     6901 ctccaggaag ccacgccagc agccccggcc ccggccgccc ccgccgcgtc gggtgcggca
     6961 gcggcggcag gcgacgccgc ggcatacgtc accccgctcg tgcgcaagct cgccgccgag
     7021 aacgacatcg acctggcgag cgtcaccggc accggcatcg gtggccggat ccggaagcag
     7081 gacatcctcg acgccgccga gaaggccaag gccgccgtcg ctccggcgac cccccaaccg
     7141 gccgcacagg caacggcacc ggccgcggcg cccgacgccg cccccgccgt tgccgacagc
     7201 cgtcgcggga cccgcgagaa gatgagccgc ctgcgcaaga tcatcgccca gcgcatggtc
     7261 gagtcgctgc aggtctcggc tcagctgacc acggtgatcg aggtcgacat gaccaaggtc
     7321 gcccggctgc gcgaccgggc caagggcgag ttcgagaagc gggagggcac caagctgtcc
     7381 ttcctgccgt tcatcgcgct cgcctcggtc gaagcgctca agacccaccc gggcgtcaac
     7441 gccagcatcg agggcgagga gatcgtctac cacggcagcg agaacctcgc gatcgcggtc
     7501 gacaccccca agggcctgat cgtgccggtc atcaagaacg ccggcgacct caatatcgcc
     7561 gggctcgccc ggtcgatcgc cgatgtcgcc gcccgcaccc gggacaacaa gatcacgccc
     7621 gacgaactgt ccggtggcac cttcaccatc accaacaccg gcagccgcgg ggcgctcttc
     7681 gacaccccga tcctcaacca gccgcaggtc gcgatgctcg gctccggcgc catcgtgcgc
     7741 cggcccgtcg tcgtcgaggt cgacggcgcc gagacgatcg cgatccgctc gatgatgttc
     7801 ctcgcgctct cctacgacca ccggatcgtc gacggcgcgg acgccgcgcg gttcctcacc
     7861 acggtcaagg agcgcctcga agagggccgc ttcgaagcct cgctggggtt gtaggcaacc
     7921 cacggcattc ggggctcgtg cgccgctaac gattcgtcgc agcaccccgg atcgcagcgg
     7981 cccaggatca ctgaccagga cgaagggacg aattccgtgc agcagggttc ggcgaggccg
     8041 ctcaccgtcg ccgtcaccgg cgcgagcggg ttgatcggcc gagcccttgt tgctgccctg
     8101 cgcgagcgtg gagacgaagt catcactctc gtgcgccggg agcccgtgag cgcgggcgaa
     8161 cgccgatggg atccggcgac cgggcacctg gacccgcagg cgctggccgg ggtggatgcg
     8221 gtggtcaatc tcgcgggccc gggcgtcggg gacaagcggt ggacaccggc atacaaggct
     8281 cgggtgctac gcgaccgggt gagcgccacc gaggcggtgg ccgaagccgt ggccgcgcgg
     8341 ctggcgagcg acgatcgagc gatccacctc gtcaacgcct ccgcggtggg ctactacggc
     8401 gaccgcgggg aggaactgct caccgaggac agccccggag gcttgggatt tctggcggag
     8461 gtatgccgcg cctgggaggg cgcgacccag gccgcgtccg cggccggtgc ccccgtgacg
     8521 ctggctcgca ccggcctggt catgtctggc gccggtggcg cgctcgcgcc gctggtgcgc
     8581 gcgacgcggc tcggcgcggg tggcccgatg ggttccgggc gccagttctg gccggtgatc
     8641 tcgcgtcgcg acgagatcgc tgccctgctc ttcctcctgg atcacccgga cctgaccggc
     8701 ccggtcaatc tcgtgggtgt cgagccgccc cgccagcgcg aagtcgccgg ggcgcttggc
     8761 cgactcctgc atcgacccgc tatccttccg gcaccgggcg ctgccctgcg ggctgtgatc
     8821 ggcgagttcg caggtgagat catggccagc cagcgggtgc tgcccgatcg gctgctcgcc
     8881 gcgggtttcg tccaccaaga ccgcgcactg accgccgtcc tcgcctcggc actggccgag
     8941 gagtgagccg agagggtttc ttacaccatg tcgcacccca cgtgttcccg ttctcatgct
     9001 gcccgccgcc tggcattgct cggcgccgcc gtcatcccgg tctcgatagc cgccggggcc
     9061 gccggcgcca gcggcgccac cgggccgacc gtcaccttga cgacgccgac cgcgggatca
     9121 accaacacgg acggccgggt gacggtcacc ggcacctatt ccggacccaa caaggtcacg
     9181 agcgtcaccg tctccgggtg ccggccggac ggttcacgat gcaaggacta cctgctcgcc
     9241 aacaccaatg gcgccttctc cggaaaatgg cgcggcttcc ccgcgcggtt gacgccgacg
     9301 tcgagcgacg gcaagtcggg caccttcacc gtggtgctga gcggcctgcc gggcacccga
     9361 ctgcggatca gcgccttcgc gagcgacgcg gtcacccctc gtggcggatc ggccacggtc
     9421 aacgtggcgg tcaaggcgcc ggaggacccg gcgttcatca gcgtcgtctt cggccgggcc
     9481 agttggaagg ccgcctccgg cgagggctgc accaatcagc cgaacaccgc gcgcaccctc
     9541 gagcaggacg ccaaggacat ggccctgctc gggctgatgg gtgtggcgca gaccgtgacc
     9601 tcacgtgtcg acgagaccga gcggctctgc cccgacaact tcgccgtcga agcgtcgtgg
     9661 gtcgacctgg cccggatgcg cgacgcctac ggctggtcgt tgacctcgca gagcaagacc
     9721 tatccggacc tgacgaagct cacgacgact caggtcatcg acgagactgc cgggacgctg
     9781 ccgatcttcc aggcacacgg tcatacccgg gcctggggtt ccttcgccta ccccaacaac
     9841 aagcagaact ccaccgtgca gcgcatcgcg accaactact tcgccttcgg gcgcaagtac
     9901 gccaacgacc tcaacagccg ccagaactcc atggtcttcc cctacgagat gcgcacgttg
     9961 tcggtcaacg ggggccgctg catcaacgtc aacctgccgt gttcgacgat gccgatggtc
    10021 aacgaccggg tgaccaccag ccccaacaac atcgggcgca tcctcaagcc cggccccggc
    10081 cactggggcg tcgtccagtt ctatcggatc gtcgagggcg tcgagggcaa gctggggcag
    10141 agcttcgcct gggactgcac ctcctcggac tggcgtaacc gctggacctc ccagcccgag
    10201 ctctactgcc gcaacacgat gatcgagggc ctcgagaagc gcaacaagag cgcaacctcg
    10261 gtcgacccgg ccgctgtcgc catcgcgtgg ggccgcaagc cgttcgggtc ctgacccgcc
    10321 accccagtcg aaagcccacg acgcgccgtc atgaattttc attacggcgc gtcgtgcact
    10381 ttcgacgtgt gggtgcccgc gtgtggacta cgcctcccgc aggtcgcgca cctgccatcg
    10441 cgaaccggac caggccaggt cgaagacgat ctgggtatgc ggtgcagccg gcaccgtcgt
    10501 cgtccgcccg ttggcgtcgg tgatggtgaa ttcgctggcg tcgaggacgg cgcggacccg
    10561 gacccggcgg tcggccccgt cgatcgcccg cgcggtggcc gaccgcaccg tgaagcgcag
    10621 accttcgtag gccaggtcgt cgcggcgggc ccgcgccagt gccgcgtcgt cggcgtccgc
    10681 ggccggtgat cccccggcgt tcagccgcac gagcgcacgc gcgtcgagat cggtccaggc
    10741 ctgcgcacgc aggtccgcga gcgcctgcac gacggccttc ggctcatcca gggcacccga
    10801 gcgggcccgg accggatcgg ccggtgccgc cgtacgcgaa cccgggtccg gagtggtccg
    10861 cgctgtcggg ctctgcgccg gcgtgctctg cgccggcgtg ctctgcggcg acgcgctcgg
    10921 gaccggctcg cgctcggacg ggccgggccc ggattgcgtg gtcgcgctgg atgccgcgaa
    10981 cggggcgggc gccagagtct gggggttatc gggtcgaagc gccagcaccg cgccggcgga
    11041 gacgagcaag accgccagcg atgccgcaag gtagcggatc gggctcgcgc acaaccaatc
    11101 ccaggcagcg agggcacgct ctccgagtcc ctcgtcgtgg gccaggccga agggatcgtt
    11161 gtcgggggcc gtctgccggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 gtccgccggt gcgcctcagt gcgagccggc ctcgggccgg cgggccggct cgccggccac
    11341 gacgggcggg tgccgcagtt gccgagtgga cgggtggacc ctccccggcg gtcgtggtcg
    11401 cgccggcgcg tggtgatcgg atcgtgaacc gggccgaaga gaaacggctc cgcgatccgg
    11461 ggtccgggtc atctgcgcgg gccagtgccc gcaacatgcc ggccaggtcg ctcgcctgcc
    11521 ggccccactc cacaggtatg cggggcgggg ccggctcgca caccgcccgg aggcgctgct
    11581 cgagatcatc cagtggctgc cccgaggtct ccacctggtg ctgccagcga accagcgcat
    11641 cggccagctc gcgccggacc tcctcgacgg cgtcgagtcc gagccgggcc acgccggcgt
    11701 cgagcggcgc gacgtcggcc aggagggtcg tccccaactg cagcagacag cgttgtgccg
    11761 caacgggatc cgggtcggcc ggccagccgc cgacatcgcc gagcaggacc gacccgtccg
    11821 cgtccaccca gaccccgccg gcgtccagca ccgggtgtcc ctgcccgcga ccggccaagc
    11881 cggccagggc ggcgtgcagg gtcaagagca cctccaccac cccactggcg cagatcggtc
    11941 cgtcggcgat caggtcctcg agagtgtggg cgggtgggtc ggcatacagc agcgcgcacc
    12001 cgcccggcac ggcgtgcacc tccgcgaggg tgagcacccc gggaaggtgt agcccggcat
    12061 acccggctcg gtcgtcctcc tcccccggac gcaccagaac ggcgacgcga gtgccatcac
    12121 tctcgcgggt gccgatccag cgggtcgatc cgtcgggtgc cgtggtcgcc ggccgcaacc
    12181 ggtaccccgt gagcgtgacg tccatcccct gccccttccg cgatccgccg gctgcgtccc
    12241 ctcaagcctc tcgcaccggc cgctcgaccc ccggaagtta tccacaacct cctggtgagg
    12301 tcacgcccgc cgtcgcgagg cgacctcctc gacaacccgg atgtcgccgg catagggggg
    12361 ctcgggccgc ggccaggagc acacgacgtc ggtgaacccc agggcggcgg cccgcccggc
    12421 ggcgtcctcg aagcagtcgg cgctgctgaa ggggtaggtc ggttgggagt ccagccccag
    12481 gtaacggcgc agcggccggt tgcggccggc gcggctcacc tccgcgtcca tgcggccggc
    12541 cgcgccggcc acgagggccc accacgctgc atcgtcatcg gccttgccgc cgtaggtgat
    12601 ccagccgtcg ccgcgctcgg ccgccagggc gattgcgcgg ggcccgttgg cggcgacgag
    12661 cagcggtatg ccgtgctgct cccccgcgtt gggtggcccg ggcaggctgc cggcaccgtc
    12721 gaggtcgtag caggggccgt ggtggctgat cccgtcctcg cggagcatcc ggtcgaccac
    12781 ggtgacgaac tcgcggaagc gggcgctgcg ggcgccggcg ctcgcgtgcg gggaggtgag
    12841 gcgcgcatcg gtgtcgccac cggcgccgac accgagcagc agccgcccac ccgagagatc
    12901 gtgcaggctc aggctctcgc ggaccagttg cgccggatgg tgggtgttgg gcgaggagac
    12961 gaaggtgccg aggcggatgc ggcgggtttc gagtgcagcg gcggcgagtg tggggacggc
    13021 ggcgaaccag ggcgagtcgg gcagcccgcc ccaggtcagg tggtcgtagg tccaggcgtg
    13081 gtcgaagccg agttcgtccg ccgacagcca gtcctggcgg gcctgcgccc acgggcgatc
    13141 ggggaggatg cagaccccga cgccgatggg ccgcgtcatg atcggtcggg atgcgcggct
    13201 tcgcccgttg acgcactcac ggcggcgtcc tcacgggcca gacgcgaggg ggcccagatg
    13261 cgcgggccga tgtcgtaggc caacgccggc accagcagcg agcgcacgat gagggtgtcg
    13321 agcaggacgc cgaaggcgac gatgaacccg atctgcacca ggaagatcag cggcagtacg
    13381 gcgagtgcgg agaaggtcgc ggccagcacg atgccggcgg acgtgatcac cccgccggtg
    13441 accgccaggc cggtgaggat gccgggccgc gtgccgcgcc gctgggcctc ctcgcggact
    13501 cgggtcatga ggaagatcga gtagtcgata ccgagggcga ccaggaagac gaacccgtag
    13561 agcggcgtcg ccgggtcgga gttggggaag tcgaggatgt ggttgaacac cagggccgcc
    13621 accccgacgg tggccgcgaa ggagaccaca ttggccacga ccaacaggac gggggccacc
    13681 agcgagcgca gcagcagggc gagcacgatg aagatgacga tcaggatcgc cgggatgatg
    13741 acccgcaggt cgcgttcggt ggcgtcgagg acgtcgaggc tgaccgccgt gctgccgccg
    13801 accagcgcgt ccggggtgac ggcgtccagg tcggcccgca gacgcctgac gacctcggtc
    13861 gcttcgggcg agtcggcggg gaccgccagg gtggcctgca cgatgtgctt gccgtcgacg
    13921 gcgaccggcg gggcgccggg cacgagaccg acgtacgggt cctggacgtc cgcctcggtg
    13981 cgaagacgct cgacgaccgc ctccgcctgc ggctgcggca cgacgatctg gaccggcgat
    14041 ccggcaccgg cggggtagtg ccgcgccagc gcctcccccg cctccaccga cggcacctcg
    14101 gtcaggaaga gttcgtcggc ggacaggccg tcggccttga aggtcgggat gaaggcggcg
    14161 aaggcaagca ggccggccag ggtgatcacc cacgtgcggc gcgggtgggt gccgaccagt
    14221 ttggccaccc ggccccacac gccgcgggtc tcgagcgcgt cttcgaggtg gcggtgatcc
    14281 agcttgggcg ccaccggcca gaagatgcgg cggccgacga gcaacaggac cgccgggagg
    14341 aaggtcaggg aggcagccat ggccccggcg atgcccaggg cgccgaccgg acccagccct
    14401 gccgtgctgc ccaggtcgga gagcatgagg cagagcaggc cgaggatgac cgtcgcgcca
    14461 ctggcgagga tcggctcgac cgcaccccgc caggccgagc gcagtgcgtc ccaggtgtcg
    14521 tcggtgtcgt gcagggcctc ccggaaccgg gacacgagca gcaatgcgta gtcggtcgcg
    14581 gcgccgacga cgaggatgaa catgatgccc tgggactggc cggacaggtc gatcgccccg
    14641 gccttggcga gcgggaagac ggccagggcg gcaagggcca aaccgaagac ggcactgatc
    14701 agcaccgcga acggcaggat cgggctgcga tagacgacga gcaggatgag caggacgacg
    14761 accagggcca cgaggagaag gacgccgtca atgccctcga aggccgtcac gaagtcgctg
    14821 agcagcccac ccggaccggc gacgtgaccg gtcaggccgg tccccttgag gtccgtgtcc
    14881 aggagcgtgc gcagttcgag tgcggtctcg tagagcgggg tggtcttgtt gatcgtgtcc
    14941 tcggccttgg cgttgtcgag gttgagcacg acgaggagtg ccttgccgtc ctggctcggg
    15001 atgggcgcgc tggtggggga gccgatgaac tcgccgatcg tgcgccctgg cccgagcttg
    15061 tcaaggggtg ccgcgggcag cttctgcact agggcggcgg cggccgcctg ctgggcgggt
    15121 gtgatggccg tgggtgactc gaggacgagg aggtagggca ggtcgttgtt cgcgctcgcc
    15181 ttctggacga agtcggccac ctcggtcgac tcggccgtgg ccggcacgaa cgaggcgttg
    15241 tcgttcttct ggacctcggc caagcggccg accagcggcc cgccgacggc ggagacggcc
    15301 agccacagca ggacgacgag ggccgcgacg agccgcggcc gccgggtgcg tcgttgctgc
    15361 ggtatggcgt gcgctccccc gctcccgacc cgcccgctca tcgcgcagcg accgaggaac
    15421 gcgccgaatc cccatgggag agcaggaagt cgagaacgac cggcagctga gcgtcccata
    15481 actcccagac gtgatcaccc ggttcgtaga gggtcgtgac gtcggcgccc acgccctgcg
    15541 ccgcggcctc gaagtcgcgc accatgggat agaggatgtc ctcggtgccg caaccgatcc
    15601 acatgggcgg caacgatctt ccgctgcgga cgagagaaaa gagatccgcc tccggcggca
    15661 cctctccggc gaagatcacc tcgttgaggc gagctcggcc gggctcgcgc atgagggccc
    15721 gcacgtccac agcccccgag agggaggcga ccgcggcata ccgctcgggg taggtcaacg
    15781 ccagccgcag cgcaccgtag ccacccatcg acagcccggc gacgaacgtc tgcgccggat
    15841 cggtgctgac ccggaagaag gtccgcacca gctgcggcag ctcctcgctg acgaaggtcc
    15901 agtagcggtg tcccccggcc tcgtcggcat agaagctgcg cgagaccgcc ggcatcacga
    15961 cggcgagccc acgggcatcg gcataccgct cgacactcgt gtagcgcgtc cacgccgagt
    16021 ggtcgtccga aagcccgtgc agcagataga gaaccggcgg gtcgcccacc cgctcgacgg
    16081 actcgactcc gacctgggcc tgcgtgacct ggggcaggac gacggtgaag gtgactccca
    16141 cctcgaggac atcggagaag cagttgatcg tcgcgacggc cacgggcggc tccttgtcct
    16201 cggtgagttc acgtgtcagc aggtgagttc accctaacct ggcgggcaga caacccgggc
    16261 cgtgacggca tacggtggct gtcatgcgct tcgagcacct gggcttcgac cccgacttcg
    16321 tggattacct ggccgcctgg gatcaccagc gagaggtcca cgcgcaggtc gtcgacgggt
    16381 cgctgcccga cacagtgttg ctgctcgaac acgccgccgt gttcaccgcc ggcaagcgca
    16441 cgctgccgct ggaacggccc ttcgacggca ctccggtgat cgatgtcgac cggggcggca
    16501 agatcacgtg gcatggtccg gggcagctgg tcggctaccc gatcgtccgg ctgccgaacc
    16561 cggtcgacgt ggtggcccac gtccgacggc tggaggaggt catgatccgc acgtgcgccg
    16621 acttcggcgt cgaggtcacc cgggtcgacg ggcgcagcgg cgtgtggatc gtcggcaccg
    16681 gcatcggcga cccagaccgc aagctcgggg ccatcggcgt gcgggtcagc cagaacgtca
    16741 ccatgcatgg cttcgccctc aacgccaacc ccgacctggg ctgggccagc gtgatcgtcc
    16801 cgtgcggcat cgctgacgcg ggcgtctcct cgctgtccat cgaggccggc cgcgagatca
    16861 cggttgcgga cgtgctgccg tataccgaga aacacctcgc cgaggtcctc accctctcct
    16921 gactcccggc ggcggcggga tgcggctcgg cgtgggggcc gcgagctcgc gcacgccgca
    16981 cgcgctgcgg tatgccgcgc gcacggccgc cccggtcaga agttggggtg tctcggggcc
    17041 cggaacgccc aggttctgac gcgagacagt cgctgctgac gcgacatggg cgatcgctcg
    17101 atgtcgatcg cccatgtcgt gacgggatgg tcgtgccggg agggttgtgt cgtgtcggaa
    17161 gggttgtcgc gtcggaaacg gttggcgtgt cggaaacggt tggcgtgtcg gaaacggttg
    17221 tcgtgtcgga agggtggtgg ggggaggggc gcggtcaggt cgtgccgttg gcggcggtgg
    17281 agtcgctggt cgccgatccg gaagtgttcg ggtcgaggct gcttccctgt tgcgaggtgc
    17341 cgtcgggagc ctgacccggc tgaccgccca gctggccgcc gtgaccgccc atgccgccgc
    17401 cggtgttctc ggtgatcgtg gccaggtcgg cgctcacctc gaagaagacg ggcgacccgt
    17461 ccttggtgcc gagcgcgtca tacgacccgt cggggtcctt gcgcacctcg gtgatcgagg
    17521 cggcggaatc cttggcctgg acggcggcga cgaccttgtc ggcctcgtcg ccggtgaccg
    17581 cggtgtcctg cgaaccgccc atctgaccat cgcccggctg gccgccgtga ccgcccgggc
    17641 ccatctgccc ctgctggccg gatgccccca ggtcggtcgt gccctggtca gtcgtgccct
    17701 gctggttcag ccccgggagc ccggagtcgg tgttcgcgct ctgcgaggtg ctcgtgctgg
    17761 gagcggtggt cgtggtgtcg tcggcgttgg cggccaggac gacgccaccc gcgaggacca
    17821 gcccggcggc ggcgccgacg gcgctggtga tgatcgtgct cttgcgtgcc atggaggcaa
    17881 ctcctgtcgt gttcggccgg cccggtgccg acctctggcc caagagactg ggcgccggac
    17941 ctaggggtgg cctgaccacg agccttcggc gggctgtgga ttcgggtccg gccctgtgaa
    18001 catcctttgg gcccaacgcc acgcggcgga accagcccac cccaatttcg ccgcgcccag
    18061 gtcgaccccg ggcgacgtag gctaagcgag tgacgatcgc ccccgaagga cgcaagctgc
    18121 tgcgggtcga ggcccgcaac gccgagacgc ccatcgagcg caaaccgagc tggatccgca
    18181 cgaccgcgaa gatgggcccg cagttcaccg agatcaacgc cctcgtcaag ggcgccggca
    18241 cgcacacggt ctgccaggaa gccggctgcc ccaacatctt cgagtgctgg gaggaccgcg
    18301 aggcgacctt cctcatcggc ggcgacacct gcacccgccg gtgcgacttc tgcgatatcg
    18361 ccaccggcaa gccgcagccg ctcgatcgcg acgagccgcg ccgggtggcg gaatcggtgg
    18421 ccaccatggg gctgcgctat tcgacggtca ccggggtggc ccgcgacgac cagcccgacg
    18481 gcgcggcgtg gctgtatgcc gagaccgtgc gccagattca cgcgctcaac cccaacaccg
    18541 gcgtcgagat cctgccgccc gacttcggcg cccaacccga gctcgtcggg caggtcttcg
    18601 acgcccgccc cgaggtcttc gcccacaacc tcgagaccgt gccgcggatc ttcaagcgga
    18661 tccgcccggc cttcacctac gagaagtccc tgcgcgtgct gacgatggcc cgcgaggccg
    18721 atctggtcac caagtccaac ctcatcctcg gcatgggtga gcaggaggcg gagatcgccc
    18781 aggccatcga ggatctgtat gccgcgggct gcgacatcct gaccatcacc caatacctgc
    18841 gcccctcccc ccgccaccac ccgatcgacc ggtgggtcaa gccggaggag ttcgtgcact
    18901 ggagcgggta tgccgagggg ctgggtttca agggcgtcat ggccggcccg ctggtccgtt
    18961 cctcctaccg ggccgggcgg ctgtgggcga gcgcgatgac caagtccggc cgcggcatac
    19021 cgccgcactt ggcgcacctg ggcgaggcgg gcgagccggc gcgccaggag gcggcgacgc
    19081 tgctcgccgg aggtgcgaag gccggcgcct aggaccgtat ccttgggagc tatggccaag
    19141 gatccggcgg cgccgaagaa gcagcgcagc aaccccttca agcagatcgc ctccgtctac
    19201 aaggcggtca agtcgatgga tccgtccatc acctggtggt tgctcggcgt cttcatcggg
    19261 gtgctggcgc tcttcgtgat catcggcttc ctcgtcgacc acccgatcta tttcgtcttc
    19321 atcggcctgc cgatcgccgc ccttgccgcg atgatcaccc tctcccggcg cggcgagaag
    19381 gccgccttcg gggcgatgca gggcaaggcc gggcagagcg tcggagcgct gtcgatgctg
    19441 cggcgcggct ggtactacga ccaggaaccc gtcgccgccg acgtcgcccg cccgcaggag
    19501 atcgccaacg ccgcgatgat cttccgcgcc ctcggcgccc cgggtgtcgt gctcatcggc
    19561 gagggcccgc tgccgcgggc gaagaagctg atggagaaag aggtcaagaa ggtcgcccgg
    19621 gtcgcccccg gcgtcccgat ccaccagctg tatgtcggtg acgacgacgg ccaggtcagc
    19681 gcccgcaagc tcacgcgcac catgaccaag ctcaagggca cgctgaccaa ggaggagatg
    19741 tccgcggtca acaaacgcct gaagtcgatc ccgggcatcc gccagggcat ccctgccggt
    19801 gtcgacccca ccaaggcccg catggaccga cgccaactgc gcgggcggta ggtcaccacg
    19861 gcactctgcg agccctgctg cccggcatac gaccgcctca tctcactctt cgggatgggg
    19921 cggtccgcgt aacacgccgg aaacaactgg gtgaaattcc attaatgcgg ccctcgtagg
    19981 gtggcgagca acgcacgggc cggcgagacg cgcccatcca ccctcacagg aggtccctct
    20041 tgttcagctc cgctgacgaa gtcctcgcgt tcatcaagga ccaggacgtc aagttcgtcg
    20101 acattcgctt ctgcgacctg cccggcgtga tgcagcactt caatgtgccc gccgagtcac
    20161 tcaccgaaga cttcttcacc gagggccagg ccttcgacgg ctcctcgatc cgtggcttcc
    20221 aggcgatcca cgagtccgac atgaagctcg tgccggacgt ggcgacggca tacctcgacc
    20281 cgttccgggc cgagaagacc ctcatcatga acttctcgat cgtcgacccc ttcaccgacc
    20341 agccctacag ccgcgacccg cgcaatatcg cgatcaaggc cgaggcctac ctgaagtcca
    20401 cggggatcgc cgacaccgca tacttcggcg cggaagccga gttcttcgtc ttcgacgacg
    20461 tgcgcttcga gaccaagccg caggcgggct actacttcat cgactccgtc gaggccgcgt
    20521 ggaacaccgg ccgcgaggag gagggcggca accgcggcta caagacccgg gtcaagggcg
    20581 gctacttccc cgtcccgccg gtcgaccact tcggcgacct gcgcgacaag atctgcctca
    20641 acatgaagga ggtcggcctg accgtcgagc gcagccacca cgaggtcggc tcggcgggcc
    20701 agcaggagat caactacaag ttcaacacgc tgctgcactc cggcgacgac atgatgaagt
    20761 tcaagtacgt cgtca
//
LOCUS       DAOPJG010000035        20075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2838_length_20075_cov_8.018182, whole genome shotgun sequence.
ACCESSION   DAOPJG010000035 DAOPJG010000000
VERSION     DAOPJG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 20075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20075
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_20075_cov_8.018182"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..747)
                     /locus_tag="PKU75_05185"
     CDS             complement(22..747)
                     /locus_tag="PKU75_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPF80305.1"
                     /translation="MPITTTTSESVATLTIDRQERRNALNHDALDELDAAVAAAVTGG
                     ARALVLTGAGGHFCAGADLTELEDVAFTRHLGAVLKRLAEVPIVSVAAIAGSCMGLGM
                     QLALACDVRVVTDDAKFAVPIAKLGLMVDHWTLDRLTRFWGEGAARQMTLTAAVLTAE
                     DAWRLGFAQVRGDLAAAQSLAAAAATLAPLTQSGTKSGFGITDPLQLSRYEAAFATAW
                     ASDDLREGRTAFTERRAPHFEGH"
     gene            complement(757..1224)
                     /locus_tag="PKU75_05190"
     CDS             complement(757..1224)
                     /locus_tag="PKU75_05190"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPF80306.1"
                     /translation="MTSITLIAAVADNRVIGNGPDIPWALPGEQARFKATTMGHTLIM
                     GRATFDSIGRPLPGRRTIVISRNPHWHHPGVETATSLEAALALAGPADEVFVAGGAQI
                     YALALPFAQRMILTEIPLSPDGDAFFPEFDAVDWAESDRETHPAYEIVTYVRR"
     gene            complement(1238..2029)
                     /locus_tag="PKU75_05195"
     CDS             complement(1238..2029)
                     /locus_tag="PKU75_05195"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPF80307.1"
                     /translation="MRPYLDLMQRVLDEGVERPDRTGTGTLGVFGHQMRFDLADGFPV
                     VTTKKLHLRSIFGELLWFLRGDTNVRWLQERGITIWDEWADENGDLGPVYGHQWRSWP
                     TPDGRSVDQIAAVIESIRTNPSSRRHIVSAWNVADVDKMALPPCHTMFQFHVAQGRLS
                     CQLYQRSADIFLGVPFNIASYALLTHLVAQQTGLEVGDFVHTLGDAHLYLNHLDQARE
                     QLAREPFPLPTLRLTPRESIDAYELEDIELVGYQAHPSIKAPIAV"
     gene            2109..2993
                     /gene="dapA"
                     /locus_tag="PKU75_05200"
     CDS             2109..2993
                     /gene="dapA"
                     /locus_tag="PKU75_05200"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPF80308.1"
                     /translation="MNLPFGRVLTAMVTPMTPDGELDATGAKAVVKHLLDTGHDGIVV
                     NGTTGESATLTSAESIEMVEWAGEAAGEAAKIVAGVGSNDTRHAVEMAKLAANAGADA
                     LLLVSPYYNKPTQAGLLAHCRAVADATDLPVLLYDIPGRTGIPFAVDTLIALAEHPNI
                     QGVKDAKGDFWAASQVMAATALQWFSGADEVNLVHLAQGATGVVSVVGHVAGKRYAEM
                     VAAVEAGDLATARRIHLALIPLVEAIMNTSQGAIMAKAALKELGVIEHASVRLPYVEG
                     PPEHLEKLRAALASTDLS"
     gene            2990..4675
                     /locus_tag="PKU75_05205"
     CDS             2990..4675
                     /locus_tag="PKU75_05205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPF80309.1"
                     /translation="MIHPHPELGLPGPLADGAVRVLPLGGLGEIGRNMTVIEHDGQLL
                     IIDCGVLFPDEHHPGIDLILPDFGPIEDRLDDVQAVVLTHGHEDHIGGVPFLLRLKPD
                     IPLLGSQLTLALVEAKLREHRIQPYTLAVTEGQRERLGVFDCEFVAVNHSIPDALAVA
                     VRTPGGTLLHTGDFKMDQLPLDGRLTDLRAFARLGEEGIDLVLTDSTNAEVPGFVTPE
                     REISPAIDKVFRHAERRIIVACFASHVHRVQQVLDAAEKAGRKVAMVGRSMVRNMGIA
                     ADLGYLHVPDGILVDFKKLGDLPDNQVVLICTGSQGEPMAALSRMANREHPIDVGPGD
                     TVLLASSLIPGNENAVYRVINGLMRLGAHVVHKGNARVHVSGHAAAGELLYVYNILSP
                     SNVMPVHGEWRHLVANGELAIATGVARDRVAVVEDGVAVDLVDGRVRVAGAFDCGYVY
                     VDGASVGAADEALLKDRRILRDEGFISIVVVVDSVSGKVVAGPDISARGFVEDAVVFD
                     DIRPALGAALDDATGNGVRDTYELQQIIRRQVGSWVGRKIRRRPMIIPIVIEA"
     gene            4870..7578
                     /locus_tag="PKU75_05210"
     CDS             4870..7578
                     /locus_tag="PKU75_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPF80310.1"
                     /translation="MATRPTSPPQRAARPAATRAGATPAKRQPPKSRPPARKPAGRAS
                     GPSLAGRAARGTGKVLGGGARLVGMGVRKVSGVDLEPEHRRDGIGLLLIALAIIVAAR
                     EWWGMPGVFGDAVHAVVAGTVGQVAFAVPLVLLWLGIRFMRATANEAANTRLAVGLSF
                     LTLASTGLVHISHGIPTPQMGVTEMRNAGGVIGFLASSPLTAGITVYGAVALLLLAGL
                     FSLLVLSATPVRQIPARLSSARAHLVRSRDTLNGEDSEHVAAGDGSPADDASPGGAGP
                     RRRRARGATAIGATAAGDGEGYEGDTAYDQAAVVAKEGRRRPRRGEPAPEPVSEPHTA
                     DASETRPATRALPTESAKDPAPAPIVPETGKPDLLPPPTAPISGRPEQLALAGDVTYT
                     LPEPTTLKEGTPPKARSAANDQVVAALTNVLDEFGIDAQVTGFTRGPTVTRYEVELGP
                     GVKVERVTSMSKNIAYAVASADVRILSPIPGKSAIGIEIPNADKEIVSLGDVLRSRTA
                     HNSTHPMTMGVGKDVEGGYVVANLAKMPHLLVAGATGAGKSSFVNSMIVSILMRSTPE
                     EVRMILVDPKRVELTAYDGIPHLITPIITNPKKAAEGLQWVVKEMDHRYDDLAAFGFK
                     HVDDFNKAVRSGAAKPLPGSERVLEPYPYLLVIVDELADLMMVAPRDVEDSIVRITQL
                     ARAAGIHLVLATQRPSVDVVTGLIKANVPSRMAFATSSLADSRVVLDQPGAEKLLGQG
                     DALFLPMGASKPIRVQGAWVTESEIHAVVDHVKTQLQANYREDVAVEAAKKQIDDDIG
                     DDLDLLLAAAELVVTTQFGSTSMLQRKLRVGFAKAGRLMDLLESRNVVGPSEGSKARD
                     VLVRPDDLAATLAMMRGEEPPAGGDVDYADEQDEGGEDAWELTGRE"
     gene            complement(7629..8675)
                     /locus_tag="PKU75_05215"
     CDS             complement(7629..8675)
                     /locus_tag="PKU75_05215"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HPF80311.1"
                     /translation="MPDASPLILATLLIVAVLIVSAVAKLREPAASASAFQMLELPKI
                     LTRLRGPRLLPLGELVLAAGLLLAPPPLRLVCAVVAAVLMLAYTAVIARALRFPYPVT
                     CGCFGTLGMGLVTVRTLWRNLLLVAVALLAIVDAVRHDRSLTAQLADVGATGLGWLAM
                     AVVTAAVAWLILDRGPAPRHTPAQPHTSAPAHHHANTPPPSTESDYIREAVPYGVLET
                     PDGRLANVREMAKSQARLLIFLSLHCHACITLREELPEFAEANPEIGVHPIYHSAVET
                     DQIPGGMDWLLDPRGGLQQSFGVYGSPCAVLLGTDGLLAGGPVSGSSDVRNFLEDIGA
                     ELAAAREAHILPQA"
     gene            8722..9417
                     /locus_tag="PKU75_05220"
     CDS             8722..9417
                     /locus_tag="PKU75_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HPF80312.1"
                     /translation="MSQLWPTPAPGRVRTNPVVRAFRERPSAILLTAQVIAMLGLAFV
                     GPSQRPVFSLVGLVILLLAIVVVRSTPALTWISGLIALPALVLEVWSIIDPEHQVVFI
                     LAHSLLAAFYGYVGYALVAYVFADHWVTRDELFAVGAAFTVICWAFAYVFFVIQELKP
                     GSFTDYSGVGQRTFHELLYLSVANFTSVGLSDVTPVQPIARGVGMIEQLTGVMYVAMV
                     ISRLVALSVIRRG"
     gene            complement(9433..10557)
                     /locus_tag="PKU75_05225"
     CDS             complement(9433..10557)
                     /locus_tag="PKU75_05225"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPF80313.1"
                     /translation="MSPLDLASLLAADPIVDGHNDLPWELRDLEGYDFDQLDIAVRGT
                     RTHTDLVRLREGGVGGQFWSVFAPGTYAPVDAVTATFEQLDCVHRMVARYADQLALAT
                     TAEQVRAAWADGRIACLLGAEGGHCIANSVGTLRMMRRLGVRYMTLTHNQNNDWADSA
                     TDEPRHGGLTDFGRDVVREMNRIGMLVDISHVAVTTMHAALDTSTVPVIASHSSARAI
                     TDHPRNVPDEVLSRLAAQGGVCMATFVPYFVNQDAADWTTAAKAAVRAAGLDPDDESS
                     RAFRESYAAAHPAPGATLADVVAHVEHLREVAGIDHIGLGGDYDGVDQLPQGLEDVST
                     YPRLLAVLAERGWSAADLAKLTSRNILRVLQAADDATAGT"
     gene            10605..12038
                     /gene="rimO"
                     /locus_tag="PKU75_05230"
     CDS             10605..12038
                     /gene="rimO"
                     /locus_tag="PKU75_05230"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPF80314.1"
                     /translation="MTVPAQRRVALVTLGCTRNEVDSEELAGRLAAEGWELVDDAAEA
                     DVAVVNTCGFIEQAKKDSIDTLLEASELKQTGRTQAVVAVGCLAERYGRELAEELPEA
                     DAVLGFDSYADLSGHLRSILAGARPESHVPADRRQLLPLTPAARREAVSSVAVPGLAQ
                     ADLAAVAPASGPPVLRRRLDDRPWAPLKIASGCDRRCAFCAIPMFRGAFVSRHPDEVV
                     AEARWLAGQGVKEVFLVSENSTSYGKDLGDLGALEKLLPRLSAVDGLDWVRVSYLQPA
                     EIRPGLLEAMLATEKVVPWFDISFQHASGPLLRRMRRFGDGDSFLELIARVRVLAPEA
                     GVRSNVIVGFPGETEEDVAELESFLTAARLDVVGVFGYSDEDGTEGLGMPGKVDEDEI
                     AARVARLQDLAELLTAQRAEERIGTVADVLIEGVEDGMPFGRTAFQGPEIDGQTSVES
                     VPDLVPGQIVAAEIVASAGVDLQAVAR"
     gene            12035..12670
                     /gene="pgsA"
                     /locus_tag="PKU75_05235"
     CDS             12035..12670
                     /gene="pgsA"
                     /locus_tag="PKU75_05235"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPF80315.1"
                     /translation="MTLPAAKVSAWNLPNTLTVMRILVVPFFGWLLLVDHGADVGLRW
                     WALALFVGAVLTDRIDGDIARRRGLVTDFGKVADPIADKAITGMAFVGLSVLGELPWW
                     ITIPVLVREWGITAMRFFIIRHSVMPAGQGGKTKTALQALALGFFIAPLWTLPEPDVW
                     MWVAWIIMAAAVILTLVTGIDIALKGRRLRQTSERTRRKRAERLARRAGGR"
     assembly_gap    12759..12858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12861..13361
                     /locus_tag="PKU75_05240"
     CDS             12861..13361
                     /locus_tag="PKU75_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HPF80316.1"
                     /translation="MTNDDAVTNDLAARVIARARHTATTLGTAESLTGGGVAHALTGV
                     PGASTVVRGGIVSYATQVKASVLGVDSRLLAERGAVDPDVALAMARGARRVLGCALAV
                     ATTGVAGPDPADGKPVGTVFVATTGPRGERVRELALSGSRTQIRSDTVAAALQAVLDT
                     LEDPPS"
     gene            13421..13726
                     /locus_tag="PKU75_05245"
     CDS             13421..13726
                     /locus_tag="PKU75_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF80317.1"
                     /translation="MILLRRELGEALREERTAQGRSLREVSAAASVSLGYLSEIERGQ
                     KEASSELLTSVCGALGVPLSDVLSDVSLRVARAEAEAAGVPTLPVTPARRGVSASAA"
     gene            complement(13892..14761)
                     /locus_tag="PKU75_05250"
     CDS             complement(13892..14761)
                     /locus_tag="PKU75_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPF80318.1"
                     /translation="MPEGDTVWRTAERLHRALAGQPLTALELRWGDLGALDLLPATTT
                     AVRARGKHLLHELDSGVTIHSHLKMEGSWRIAATAEVTRRTRANPQLRILAETAAWSA
                     LGLRLGLVEVWPSSESATRLAYLGPDILGPDWDPESAVANLHRQPATAIGVALLDQRN
                     LAGIGTFWAAEALFRYRRNPWTAAAESTRDDLESLVEWTQGMMRRSAATGHQTSTGRT
                     ERDATAYVHGRSGRPCRRCGTTVRVAPLGQAPRARVFFYCPSCQGGLAPTDEGTTQVP
                     LGASGVKSPRRRR"
     gene            complement(14840..19573)
                     /locus_tag="PKU75_05255"
     CDS             complement(14840..19573)
                     /locus_tag="PKU75_05255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPF80319.1"
                     /translation="MTADVLDRFSPATQAWFRGSFPAPTPAQQGAWEAISSGKDALVV
                     APTGSGKTLSAFLWAIDGLVREPVPDDPLARCRVVYISPMKALAVDVERNLRAPLVGI
                     GHAADRLGLPRPDVSVAVRSGDTPANERRAFAKRPSDILITTPESLFLLLTSNARDAL
                     AGVETVILDEVHAVAATKRGAHLAVSLERLDALLPKPAQRIGLSATVRPIEEVARFLS
                     GGRPVEVVAPPSAKEWDLQVVVPVPDMGSLGEPTGDLAGAAAGPERRASIWPHVEERI
                     VDLVEGERSTLVFANSRRLAERLTARLNEIWDERLLAAEQDASILAEDASLEAVEGMR
                     SRPARTPAQVMAQAGGSAGAPPVLARAHHGSVSKEQRLDIEEALKAGRLPAVVATSSL
                     ELGIDMGSVDAVVQVESPPSVAAGLQRVGRAGHQVGAISRGVFFPKYRGDLVQTAVVV
                     ERMRAGAIEALAVPANPLDVLAQQVVAMTAMDTWEVSALHTLLRRAAPYAGLSRTVLE
                     SVLDMLAGRYPSEEFAELRPRITWDRLADTVIARKGGQHLAVTSGGTIPDRGLFGVFL
                     ASDGPGRRVGELDEEMVYESRVGDVFTLGTSSWRIEEITRDQVLVTPAPGEPGKLPFW
                     KGETQGRPVELGQAVGSFVRELSGLTPKKALARASAAGLDPWAADNLLAYLGEQKEAT
                     GVVPSDTTIVVERFRDEIGDWRVAVHSPFGMPVHAPWALAIGARMRERFGVEVQAMAA
                     DDGIVLRLPDIEFDDGSDAGAELGELLFLHPDEVDALVTAQLPGSALFASRFRECAAR
                     ALLLPRRRPDQRQPLWQQRQRAAQLLTVAANYPDFPIVLETVRECLQDVYDVPGLVDL
                     MRSVAARRITTVDVHSTQPSPFARSLMFGYVAQYLYEGDSPLAERRAAALSLDPALLA
                     ELLGRGEALALRELLDVGAITTTSAQLQRLTPERAARNADEVTDLLRILGPLRRDDLA
                     SRSDPELAAILDATLTALVAQRRVIPVRIAGDERYAVAEDAGRLRDALGTALPVGLPA
                     AFLEPVADPLGELVARYARTHTPFLLTDLAAEFGIGTAVAHDALRRLAAAGRIAEGEF
                     LPAGAGTEFCDAEVLRLLRRRSLASLRAEVEAVDARQYARFLPGWQGVGSGRMRGSEG
                     VLRVVEQLSGALLPASAIESLVLPARVTDYQPAFLDELMSAGDVLWQGHGSLPGDDGW
                     VSLHLAESAPWTLHPAAADGADPKKTATESDSAASGPSLAGSADAVALREVFAAGGAH
                     FFRGLVSAAEVDSDRDALDALWELVWRGEVTGDTFAPVRALLAGGRGAHKQRPKPAPR
                     STRYGRRGSLAGIPRSSGLARAGGMSPSGPPTAVGRWSLLPSVDLDPTTRTLTTAEVL
                     LDRYGVLTRSIVAAEEVPGGFAGIYRVLSAAEETGRLRRGYFIESLGAAQFATAGVID
                     ALRSAPADAPALVLAATDPANPYGAALPWPARPEAGGHQPGRKAGAVVVLVDGELILY
                     VERGGKTMLSWPAADEATGEATGDATVQAAADALALAVRAGALGRLTVEKADGTSVLA
                     AGNPVAEALARSGFTLTPRGLRLRR"
     gene            complement(19802..>20075)
                     /locus_tag="PKU75_05260"
     CDS             complement(19802..>20075)
                     /locus_tag="PKU75_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80320.1"
                     /translation="HPTSYMSDCSRPAGPGSGTRALAAAVEMVDTSRLVNLNDVICPG
                     GTCWPVIGNVLVYRSGSHITATFVNTLTDTLATRLDIALTDLGVRH"
BASE COUNT         2916 a         6903 c         7275 g         2881 t
ORIGIN      
        1 catggtgatg ggtctcgggc attagtggcc ctcgaagtgg ggggcgcggc gttcggtgaa
       61 ggcagttcgg ccttcgcgca ggtcatcgct cgcccaggca gtggcgaacg cggcctcgta
      121 gcgggagagc tggagcgggt cggtgatgcc gaagccggac ttggtgccgg actgggtgag
      181 cggcgccagg gtcgcggcgg cggccgcgag cgactgcgcg gccgccaggt cgcctcggac
      241 ctgcgcgaat cccagccgcc acgcgtcctc cgccgtgagt accgccgccg tgagcgtcat
      301 ctggcgcgcg gcgccttcgc cccagaaacg ggtgaggcgg tcgagggtcc agtggtcgac
      361 catgagaccg agcttggcga tcggcaccgc gaacttcgca tcgtcggtga ccacgcgcac
      421 atcgcaggcg agggcgagct gcatacccag tcccatgcac gatccggcga tcgccgccac
      481 cgacacgatc ggcacctcgg cgagccgctt gagcacggcg ccgaggtggc gggtgaaggc
      541 gacgtcttcg agttcggtca ggtcggctcc ggcgcagaag tgcccgcccg cgccggtgag
      601 cacgagcgcc cgggcgcccc cggtcaccgc cgcggccacc gccgcatcca gttcgtccag
      661 cgcgtcgtgg ttgagcgcgt tgcgccgctc ctggcggtcg atcgtgaggg tcgcgacgga
      721 ctcggacgtg gtcgtggtga tcggcaccct gcgagcctaa cgccgcacgt aggtgacgat
      781 ttcgtatgcc gggtgcgtct cccggtcgct ctcggcccag tcgacggcgt cgaactcggg
      841 gaagaaggcg tcgccgtcgg gggagagggg gatctcggtg aggatcatcc gctgggcgaa
      901 cggcagggcg agggcataga tctgggcgcc gccggcgacg aagacctcat ctgccgggcc
      961 cgcgagggcc agggcggcct cgagactggt ggccgtctcg acgccggggt ggtgccagtg
     1021 cgggttgcgg gagatgacga tggtgcgccg cccgggcaac ggccgcccga tggagtcgaa
     1081 ggtggcgcgg cccatgatca gggtgtggcc catcgtcgtg gccttgaagc gcgcttgctc
     1141 gccgggcagc gcccagggga tgtcgggccc gttgccgatg acgcggttgt cggcgacggc
     1201 ggcgatgagg gtgatcgacg tcatcgggtt gcgggcgtca tacggcgatc ggcgccttga
     1261 tcgacgggtg ggcctggtag ccgaccagct cgatgtcctc caactcgtag gcgtcgatgg
     1321 actcccgcgg cgtcaatcgc agggtcggca gcgggaaggg ctcgcgggcc agctgctcgc
     1381 gggcctgatc gaggtggttg agatagaggt gggcatcgcc gagggtgtgc acgaagtcgc
     1441 cgacctcaag cccggtctgc tgggcgacga ggtgggtgag cagggcatac gaggcgatat
     1501 tgaacgggac accgaggaag atgtcggcgc tgcgctgata gagctggcac gacagtcggc
     1561 cctgcgcgac gtggaactgg aacatcgtgt ggcacggcgg caaggccatc ttgtcgacat
     1621 cggcgacatt ccacgccgag acgatgtgcc gccggctgct ggggttggtg cggatcgact
     1681 cgatcacggc agcgatctgg tcgacgctgc gcccgtccgg ggtcggccag ctgcgccact
     1741 gatggccata gaccgggccg agatctccgt tctcgtccgc ccactcgtcc cagatcgtga
     1801 tgccccgctc ttgcagccag cgcacattgg tatcgccgcg caggaaccac aacagctccc
     1861 cgaagatcga gcgcaggtgc agcttcttgg tggtcacgac ggggaacccg tcggcgaggt
     1921 cgaagcgcat ctggtggccg aagaccccga gcgtgcccgt gccggtgcgg tccgggcgct
     1981 ccacaccctc gtcgaggact cgttgcatca ggtcgaggta ggggcgcatg gcggcagcct
     2041 agattccggg tccgacaacg cgcgagatct cccgcccact gtcggggcca gcgcataccc
     2101 ttgagcgcat gaacctgccc ttcggacgcg tcctgaccgc gatggtgacc ccgatgaccc
     2161 ccgatgggga gctcgacgcc accggagcca aggcggtcgt gaagcacctg ctcgacaccg
     2221 gtcacgacgg catcgtcgtc aacggcacga cgggtgaatc ggccaccctc acctcggccg
     2281 agtcgatcga gatggtcgag tgggcgggag aggccgccgg agaggcggcc aagatcgtcg
     2341 ccggggtggg cagcaatgac acccggcatg cggtggagat ggccaagctc gccgccaacg
     2401 ccggcgccga tgcgctgttg ctcgtctcgc cctactacaa caagcccacc caagccggcc
     2461 tccttgccca ctgccgcgcc gtcgccgacg ccaccgacct gccggtcctc ctctatgaca
     2521 tccccggccg cacgggcata ccgttcgccg tcgacaccct gatcgcgctc gcggagcacc
     2581 ccaacatcca gggcgtcaag gacgccaagg gcgacttctg ggccgcgtcc caggtgatgg
     2641 cggcgacggc cctgcaatgg ttctccggcg ccgacgaggt caacctcgtc cacctcgcgc
     2701 agggggcgac gggtgtggtc agcgtcgtcg ggcatgtcgc cggcaagcgg tatgccgaga
     2761 tggttgccgc cgtcgaggcc ggtgacctcg cgacggcgcg gcggatccac ctggccctca
     2821 tcccgctcgt cgaggcgatc atgaacacca gccagggcgc gatcatggcc aaggcggcgc
     2881 tgaaggagtt gggtgtcatc gagcacgcca gcgttcgact gccgtacgtc gagggcccgc
     2941 ccgaacacct cgagaagctg cgcgccgctc tcgcgtcgac cgacctgtcg tgatccaccc
     3001 gcaccccgaa ctcggtctgc ccgggccgct cgccgacggc gccgtgcgcg tcctgcctct
     3061 cggtgggctc ggcgagatcg gccgcaatat gacggtcatc gagcacgacg gccagctgct
     3121 gatcatcgac tgcggtgtgc tcttccccga cgagcaccac ccgggcatcg acctgatcct
     3181 gcccgacttc gggccgatcg aggaccgcct cgacgacgtc caggcggtcg tgttgacgca
     3241 cgggcacgag gaccacatcg gcggcgtgcc cttcctgctg cggctcaagc ccgacatacc
     3301 gctgctcggg tcccagctga cgctggcgct ggtcgaggcc aagctcaggg aacaccgcat
     3361 ccagccctac acgctggcgg tgaccgaggg gcagcgggag cggctcggcg tcttcgactg
     3421 cgagttcgtc gccgtcaacc actccatccc cgacgcgctc gccgtggcgg tgcgcacccc
     3481 gggcgggacc ctgctgcata ccggcgattt caagatggac cagctgccgc tcgacgggcg
     3541 gctgaccgac ctgcgggcgt tcgcgcggct gggagaggaa ggcatcgacc tggtgctgac
     3601 cgactcgacc aacgccgagg ttccggggtt cgtcacgccc gagcgggaga tctcaccggc
     3661 gatcgacaag gtcttccggc acgccgagcg acggatcatc gtcgcgtgct tcgcctcgca
     3721 cgtccaccgg gtccagcagg tgctcgacgc ggccgagaag gccgggcgca aggtggcgat
     3781 ggtgggccgg tcgatggtgc gaaacatggg gatcgccgcc gacctgggct atctgcacgt
     3841 gcccgacggg atcctggtcg acttcaagaa gctcggcgat ctgccggaca accaggtggt
     3901 gctcatctgt accggcagcc agggtgagcc gatggcggcg ctgtcgcgga tggccaaccg
     3961 ggagcacccg atcgatgtcg ggcctggcga caccgtgttg ctcgcctcct ccctcatccc
     4021 cggcaacgag aacgccgtct atcgggtcat caacggcctg atgcgcctcg gcgcccatgt
     4081 cgtgcacaag ggcaacgccc gagtccacgt ctccgggcac gcggccgccg gggaattgct
     4141 ctacgtctac aacatcctct cgccgagcaa tgtcatgccg gtgcacgggg agtggcgcca
     4201 cctggtcgcc aacggcgagc tggccattgc caccggcgtg gcccgagacc gggtcgccgt
     4261 cgtcgaggac ggcgtagccg tcgacctggt cgacgggcgg gtacgggtgg cgggcgcatt
     4321 cgactgcggc tacgtctacg tcgacggcgc ctcggtcggc gcggccgacg aggcgctgct
     4381 gaaggaccgg cgcatcctgc gcgacgaggg tttcatctcc atcgtcgtgg tcgtcgactc
     4441 cgtcagcggc aaagtcgtcg ccgggcccga tatctccgcg cgcgggttcg tcgaggatgc
     4501 tgtggtcttc gacgacatcc gcccggcgct gggggccgcc ctcgacgatg ccaccggcaa
     4561 cggggtgcgc gacacctacg agctgcagca gatcatccgc cgccaggtcg gcagctgggt
     4621 cggccgcaag atccgtcgcc gcccgatgat catccccatc gtcatcgagg cctgatcgac
     4681 gtcccctcag gacgcgaggg gtggacgcga gcacggggag ggtcccgccg atccagtcga
     4741 gtggggattc gcggcacgct ttgtcgtcga aagcgtaccg acgttcccct ctcgaccggt
     4801 cgacccaacc tgggagcgct ccgcgtgtga cgggtgggaa tcctgaggcg atcgcgtacc
     4861 tttaggacca tggcgacccg tccgacctca ccgccgcagc gtgctgcgcg cccggccgcg
     4921 acccgcgccg gcgcgacccc cgccaagcgg cagcctccca agagccgccc gccggctcgc
     4981 aagcccgccg gccgcgcgag cgggccgtca ctcgccggcc gcgccgcccg cggcaccggc
     5041 aaggtgctcg gtgggggcgc gcgcctggtc gggatggggg tccgcaaggt cagcggggtc
     5101 gacctggagc cggagcaccg ccgcgacggc atcgggctgc tgctcatcgc cctcgcgatc
     5161 atcgtcgcgg cccgcgaatg gtggggtatg ccgggtgtct tcggcgacgc cgtgcacgcc
     5221 gtggtcgccg gcaccgtcgg ccaggtcgcc ttcgccgtgc ccctggtgct gctgtggctc
     5281 ggcatccgat tcatgcgcgc aaccgccaac gaggccgcca acacccgcct cgcggtcggc
     5341 ctgagcttcc tcaccctggc cagcaccggc ctggtgcaca tctcgcacgg catccccacc
     5401 ccgcagatgg gtgtcaccga gatgcgcaac gccggcggcg tcatcgggtt cctcgcctcc
     5461 agccccctca ccgcgggcat cacggtgtac ggcgcggtcg ccctgctcct gctcgccgga
     5521 ctcttctcgc tcctcgtcct gtcggccacg cccgtccggc agatcccggc ccgcctcagc
     5581 agtgctcgcg cccacttggt gcgctctcga gacaccctca acggtgagga ctccgagcac
     5641 gtcgccgctg gggatggctc gcccgccgat gacgcctccc cgggcggcgc ggggccgcgc
     5701 cggcgccgag cccgcggcgc caccgcaatc ggcgccaccg cagccggtga cggcgagggc
     5761 tatgaggggg acacggcata cgaccaggcg gccgtggtgg cgaaggaagg acggcgccgc
     5821 ccccgccgcg gcgagccggc acccgaaccg gtgagcgagc cgcataccgc agacgcatcc
     5881 gagacccgcc ccgccacccg cgccctgccg accgagtctg ccaaggaccc ggcaccggcc
     5941 cccatcgtcc cggagacggg caaacccgac ctgctgccgc cgcccaccgc accgatctcc
     6001 ggccgaccgg agcagctcgc gcttgccggg gacgtcacct acaccctgcc cgagccgacc
     6061 accctcaagg agggcacgcc gccgaaggcg cgctcggccg ccaacgacca ggtcgtcgcc
     6121 gcactcacga atgtgctgga cgagttcggc atcgacgcgc aggtcaccgg cttcacccgc
     6181 ggcccgacag tcacccgcta cgaggtcgag ctcggccccg gcgtcaaggt cgagcgggtc
     6241 acctcgatgt ccaagaacat cgcctacgcg gtagcgagcg ccgacgtgcg catcctcagc
     6301 cccatccccg gcaagtccgc catcggcatc gagatcccca acgcggacaa ggagatcgtc
     6361 tccctcggcg acgtgctgcg cagccgcacc gcgcacaaca gcacccatcc gatgacgatg
     6421 ggcgtcggca aggacgtcga gggcggttat gtcgtggcga acctcgccaa gatgccgcac
     6481 ctgctcgtcg ccggcgccac cggcgcgggc aagtcgagct tcgtcaactc gatgatcgtc
     6541 tcgatcctca tgcgctccac gccggaggag gtgcggatga tcctcgtcga ccccaagcgg
     6601 gtggagctga cggcctacga cggcatcccg cacctgatca ccccgatcat caccaacccc
     6661 aagaaggccg ccgaggggct gcaatgggtg gtcaaggaga tggaccaccg gtatgacgac
     6721 ctggccgcct tcgggttcaa gcatgtcgac gacttcaaca aggccgtccg gtccggtgcc
     6781 gccaagccgc tccccggcag cgagcgcgtg ctggagccct atccctatct gctggtgatc
     6841 gtcgacgagc tcgccgacct gatgatggtc gccccgcgcg atgtcgagga ctcgatcgtg
     6901 cggatcaccc agctggcccg ggccgcgggc atccacctcg tgctggccac gcagcggccg
     6961 tcggtcgatg tcgtgacggg cctgatcaag gccaatgtgc cgagccgtat ggcgtttgcc
     7021 acctcctcgc tcgccgacag ccgggtggtg ctcgaccagc cgggcgcaga gaagctgctg
     7081 gggcagggtg atgcgctgtt cctgccgatg ggggcgagca agccgatccg tgtccagggc
     7141 gcctgggtga ccgagtccga gatccacgcg gtcgtcgacc atgtcaagac gcaactgcag
     7201 gccaactacc gcgaggatgt cgcggtcgag gcggccaaga agcagatcga cgacgacatc
     7261 ggcgacgacc tcgacctgct gctcgccgcg gccgagctgg tcgtaacgac gcagttcggg
     7321 tcgacctcga tgctgcagcg caagctgcgg gtcggcttcg ccaaggccgg ccgcctgatg
     7381 gacctgctgg agtcccgcaa tgtcgtcggg ccctccgagg gctcgaaggc gcgcgatgtg
     7441 ctggtgcgcc ccgatgacct ggcggccacg ctggcgatga tgcgcggcga ggaaccgcca
     7501 gccggcggag atgtcgatta tgccgacgag caggacgagg gcggcgagga cgcctgggag
     7561 ctgaccgggc gggagtgacg gggccggttt ccctaggcaa cccgcccgcg tgacggggtc
     7621 ctgggtggtc aggcctgcgg caggatgtgc gcctcgcgag cggcggcgag ctcggcgccg
     7681 atgtcctcga ggaagttgcg cacatcgctc gagcccgaca ccggtccgcc ggcgagcaat
     7741 ccgtcggtgc ccagcagcac ggcacacgga gatccgtaga cgccgaaaga ctgctgcagc
     7801 ccgccgcgcg ggtcgagcag ccagtccatg cccccgggga tctggtcggt ctcgacggcg
     7861 ctgtgataga tggggtgcac gccgatctcg gggttggcct cggcgaactc cggcagctcc
     7921 tcgcgcaagg tgatgcacgc gtggcagtgc aggctgagga agatcagcag ccgcgcctgg
     7981 ctcttcgcca tctcgcgcac attggcgagg cggccgtccg gggtctccaa cacgccatac
     8041 ggcactgctt cccggatgta gtcgctctcg gtgctgggcg gtggcgtgtt cgcgtggtgg
     8101 tgcgccggtg cagaggtatg cggctgggcc ggggtatgcc gtggcgctgg tccgcggtcg
     8161 aggatcagcc aggccacggc cgcggtcacc acggccatgg cgagccagcc gagccccgtg
     8221 gccccgacgt cggcgagttg ggcggtcagg ctgcggtcgt ggcgcacggc gtccacgatc
     8281 gccagcagcg ccacggcgac gagcagcaga ttgcgccaca aggtgcgcac ggtgaccagc
     8341 cccatcccga gcgtgccgaa gcagccgcac gtcacgggat acgggaagcg cagcgcacga
     8401 gcgatcacgg cggtgtaggc gagcatcagc acggcggcca ccacggcgca gacgagccgc
     8461 agtggcggcg gggcgagcag gaggcccgcc gcgagcacca actcgcccaa cggcagcagc
     8521 cgcggcccac gcagacgggt caggatcttc ggcagctcca gcatctggaa ggcgctcgcg
     8581 gacgcggccg gctcacgcag cttcgcgacc gcgctgacga tcagaaccgc cacgatgagc
     8641 agagtcgcaa ggatgagcgg ggacgcatcg ggcacgcggc aagtatagga aggaggccgg
     8701 atcaccttag gctgtcaggc catgtcccag ttgtggccga cgcccgcacc cgggcgcgtc
     8761 cggaccaacc ccgtcgtccg cgcgttccgg gagcggccca gcgcgatcct gctcactgct
     8821 caggtcatcg cgatgctcgg gttggcgttc gtcggcccga gccagcggcc ggtgttctcg
     8881 ctcgtcgggc tcgtcatcct gctgctcgcg atcgtggtgg tgcgttcgac acccgccctg
     8941 acctggatct cggggttgat cgcgctgccg gcgctggtgc tcgaagtgtg gagcatcatc
     9001 gacccggagc atcaggtcgt cttcatcctg gcgcacagcc tgctcgcggc cttctacggc
     9061 tacgtcggct atgccctggt ggcctatgtc ttcgccgacc actgggtcac ccgcgacgaa
     9121 ttgttcgccg tgggcgccgc tttcacggtc atctgctggg cgttcgccta tgtcttcttc
     9181 gtcatccagg agctcaagcc cggatcgttc accgactact ccggggtggg gcagcggacc
     9241 tttcacgagt tgctctatct gtcggtggcg aacttcacga gcgtgggcct gtccgacgtg
     9301 accccggtgc agccgatcgc ccgtggggtg ggcatgatcg agcaactcac gggcgtgatg
     9361 tacgtggcga tggtgatctc ccggctcgtc gcgctgtcgg tgatccggcg cggctgaggg
     9421 ccgtatgccg tctcaggtgc cggcggtcgc gtcgtcggcg gcctgcagca cgcgcaggat
     9481 gttgcggctg gtgagctttg ccagatcggc ggccgaccag ccgcgctcgg ccagcaccgc
     9541 caacaaccgc ggataggtcg agacgtcctc cagcccttgc ggcagctgat cgacgccgtc
     9601 gtaatccccg ccgaggccga tgtggtcgat cccggccacc tcccgcaggt gttcgacgtg
     9661 ggcgaccaca tcggcgagcg tggcgccggg cgcggggtgg gcggcggcat acgactcccg
     9721 gaacgcccgg ctgctctcgt cgtccgggtc gagccccgcg gcgcgcaccg cagccttggc
     9781 agcggtcgtc cagtcggcgg cgtcctgatt gacgaaatag gggacgaagg tcgccatgca
     9841 caccccgccc tgggcggcca gccgggacag gacctcgtcg ggcacattgc gcgggtggtc
     9901 ggtgatcgcg cgggcggagg agtgggaggc gatgacgggc acggtgctgg tgtccagcgc
     9961 cgcgtgcatc gtcgtcacgg cgacgtgtga gatgtcgacg agcatcccga tgcggttcat
    10021 ctcgcgcacg acgtcgcggc cgaagtccgt caggccaccg tggcgcggct cgtcggtcgc
    10081 cgagtcggcc cagtcgttgt tctggttgtg cgtcaacgtc atatagcgca ccccgagccg
    10141 gcgcatcatc cgaagtgtcc caacggaatt ggcgatgcag tgtccgccct cggcacccag
    10201 caggcaggcg atccgcccgt ccgcccaggc ggcgcgcacc tgctcggcgg tggtcgccag
    10261 agccagctgg tcggcatacc gtgccaccat gcggtgcacg cagtcgagct gctcgaacgt
    10321 ggccgtcacg gcgtcgaccg gcgcataggt gccgggcgcg aacaccgacc agaactgccc
    10381 cccgacgccg ccctcccgca gacgcaccag gtcggtatgg gtgcgggtgc cgcgcaccgc
    10441 gatgtcgagc tggtcgaagt cgtagccctc cagatcgcgc aactcccacg gcaggtcgtt
    10501 gtgcccgtcg acgatggggt cggcggccag cagggaggcg aggtcgaggg gactcatgac
    10561 atgcaacgct aggggatgcg cgacggcccc atagagttga ccgtatgacg gttcccgcgc
    10621 agcgccgcgt cgcgttggtc accctcgggt gcacccgcaa cgaggtcgac tccgaggaac
    10681 tcgccgggcg gctcgcggcc gagggctggg aactcgtcga cgacgcagcc gaggccgatg
    10741 tcgccgtggt caacacgtgc ggcttcatcg agcaggccaa aaaggactcg atcgacacgc
    10801 tgctggaggc cagcgagctg aagcaaacgg ggcgcacgca agccgtcgtc gccgtgggct
    10861 gcctcgccga gcggtatggc cgcgagctcg ccgaggagtt gccggaagcc gacgcggtgc
    10921 tcgggttcga ttcgtatgcc gacctgtcgg gtcacctccg ctcgatcctc gccggggcgc
    10981 ggccggagag ccacgtgccg gccgatcgcc ggcagctgct gccgctcacg ccggccgcgc
    11041 gccgggaggc ggtgtcgtca gtggccgtgc cggggctggc gcaggccgac ctggctgccg
    11101 tcgcgccggc gagcggaccg ccggtgctgc gccggcgcct ggacgaccgg ccgtgggcgc
    11161 cgctgaagat cgccagcggc tgcgatcggc gctgcgcctt ctgtgcgatc ccgatgttcc
    11221 ggggtgcctt cgtctcccgg cacccggatg aggtcgtcgc cgaggcccgg tggctcgccg
    11281 gccagggggt caaggaggtc ttcctcgtca gcgagaacag cacctcctac ggcaaggacc
    11341 tgggcgacct gggcgccttg gagaaactgc tgccgcgcct gtcggcggtc gatgggctgg
    11401 actgggtgcg cgtgtcctac ctgcagccgg cggagatccg ccccggactg ctcgaggcca
    11461 tgttggcgac cgagaaggtc gtgccgtggt tcgacatctc cttccagcac gcatcggggc
    11521 cgctgctgcg gcggatgcgg cgcttcggcg acggcgactc cttcctcgag ttgatcgccc
    11581 gggtgcgtgt cctggccccc gaggcgggcg tccggtccaa cgtcatcgtc gggttccccg
    11641 gggagaccga ggaggacgtg gcggagttgg agtccttcct gacggcggcc cggctcgatg
    11701 tcgtgggcgt cttcggatat tccgacgagg acggcaccga gggtctgggc atgccgggca
    11761 aggtcgacga ggacgagatc gccgctcggg tggcccgctt gcaggacctc gcggagttgc
    11821 tgacggcgca gcgggccgag gaacgcatcg gcacggtcgc cgacgtgctc atcgagggcg
    11881 tcgaggacgg tatgccgttc ggccgcaccg cttttcaggg tccggagatc gacgggcaga
    11941 cgagtgtcga gtcggtgccg gacctggtgc cgggccagat cgtggcggcc gagatcgtcg
    12001 cgagcgcggg tgtcgacttg caggcggtcg cccgatgacc ctgcccgccg ccaaggtctc
    12061 ggcgtggaat ctgcccaaca cgctcaccgt gatgcgcatc ctcgtcgtgc ccttcttcgg
    12121 atggctgctg ctcgtcgatc acggtgccga tgtcgggctg cgctggtggg cgctggcgct
    12181 cttcgtcggt gcggtcctga ccgaccgcat cgacggtgac atcgctcgtc ggcgcgggct
    12241 ggtgacggac ttcggcaagg tcgccgaccc gatcgccgac aaggcgatca ccgggatggc
    12301 gttcgtcggc ctctccgtgc tcggcgaact gccgtggtgg atcaccatcc cggtcctggt
    12361 gcgggagtgg ggcatcaccg cgatgcgctt cttcatcatc cggcacagcg tcatgccggc
    12421 cgggcagggc ggcaagacca agacggcgct gcaggctttg gcgctggggt tcttcatcgc
    12481 tccgctgtgg acgttgccgg agccggatgt gtggatgtgg gtggcctgga tcatcatggc
    12541 cgccgcggtc atcttgaccc tcgtgaccgg catcgacatc gcgctcaagg ggcgccggct
    12601 gcgacagacc agcgagcgca cccggcgcaa gcgcgccgag cgcctcgccc ggcgcgccgg
    12661 cgggcgatga ccgccaccgg tgacgcggcc cccggtccgg cgacggacgg cgcccccgcg
    12721 gtcacgaacg agctgacggt cacgaacgag ctgacggtnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnncg gtgacgaacg acgacgcggt cacgaacgat ctggcggcgc
    12901 gggtgatcgc acgcgcacgg cataccgcca ccacgctggg caccgccgag tccctcaccg
    12961 gtggcggcgt ggcccacgcc ctgaccggcg tgcccggtgc atcgacggtc gtgcgtggcg
    13021 gcatcgtgtc ctatgcgacg caggtcaagg caagcgtgct gggcgtcgac tcccggctgc
    13081 tcgccgagcg cggcgccgtc gatcccgacg tcgcgctggc catggcccgc ggggcccggc
    13141 gggtgctggg ctgtgccctc gcggtggcga cgaccggggt ggccggcccg gacccagccg
    13201 acgggaagcc tgtgggcacc gtctttgttg ccactactgg cccccggggt gagcgggtgc
    13261 gcgaacttgc cctatcgggg tcgcggacac agatccggtc ggatacggtg gctgcagcac
    13321 tccaggcggt gctcgacacg ctcgaggacc caccctcgtg accggtgtcg gtcgcgaccg
    13381 ttatctttcc ccacaagcga cgagacgatg aggagcccag atgatcttgc ttcggcgtga
    13441 gctgggcgaa gccttgcgcg aagagcgcac cgcccagggc cgctccctgc gcgaggtctc
    13501 ggctgccgcc tcggtctccc tcggctacct cagcgagatc gaacgtggcc agaaggaagc
    13561 ctcgtccgaa ctgctgacct ccgtatgcgg tgccctcggc gtccccctgt ccgacgtcct
    13621 gagcgatgtc agcctgcgcg tcgcacgcgc cgaggccgaa gccgccggcg tcccgaccct
    13681 cccggtcacc cccgcccgcc gcggcgtctc cgcctccgcg gcctgacggc tcgcggagag
    13741 ccctcgccgg gaggcgatcc ccgaccgcaa gagccgcgag acgacgcagt tcgccgggct
    13801 cgcctaggcc accccacgac gtgtcctcgc gccctcgtcg ctccggtact tcgcggggac
    13861 ccgtcacgtc ggtggcttgt cgctactcgt cctagcggcg ccgacggggt gacttgacgc
    13921 cgctggcgcc gagtggcacc tgggtggtgc cctcgtcggt cggggcgagg ccgccttggc
    13981 aggacgggca atagaagaac acgcgcgcgc gtggtgcctg accgagaggg gcgacccgga
    14041 ccgttgtgcc gcaccggcgg cagggtcgtc ccgatctgcc atggacgtat gccgtggcgt
    14101 cgcgttcggt ccgcccggtc gaggtctggt ggccggtagc ggcgctacgg cgcatcattc
    14161 cctgcgtcca ctcgaccaga ctctcaagat cgtcgcgggt gctctcggcg gcggccgtcc
    14221 acgggttgcg tcggtagcgg aacagggctt ccgcggccca gaacgtgccg attccggcga
    14281 gattgcgctg atcgagcagg gccacgccga tggcagttgc cggctggcgg tggaggttcg
    14341 ccacggcact ctcggggtcc cagtcagggc cgaggatgtc cgggccgagg taggcgagcc
    14401 gggtggcgga ctcggaggac ggccatacct cgaccaggcc caggcgcaat ccgagggcac
    14461 tccaggccgc cgtctcggca aggatgcgaa gctgcggatt cgcgcgggtc cgtcgagtca
    14521 cctcggccgt cgcggcgatc cgccagctgc cttccatctt caggtgcgag tggatcgtga
    14581 cgccggagtc cagctcgtgg agcaggtgtt tgccgcgggc acggacggcg gtggtcgtcg
    14641 ccggcaacag gtcgagcgcc cccagatcgc cccagcgcag ttcgagcgcg gtcagcggct
    14701 gaccggccaa tgcccggtgg aggcgctcgg cggtgcgcca gaccgtgtca ccttcgggca
    14761 ttgcagcatt ctgccgtctc ggcgtggccg accctcggat tccacgaggc catcaacacg
    14821 gccccgccac gggcggcgct cagcggcgca aacgcaagcc gcgaggggtc agggtgaagc
    14881 cggaccgggc cagtgcctcg gcgacgggat tgccggcggc gagcacggag gtgccgtcgg
    14941 ccttctcgac ggtgagccgg ccgagcgcgc cggcgcgcac ggccagggcg agggcgtcgg
    15001 cggctgcctg gaccgtcgcg tcaccggtcg cttcgccggt cgcctcgtcg gccgcgggcc
    15061 aggacagcat ggtcttgccg ccgcgctcga catagaggat cagttcgcca tcgacgagga
    15121 cgacgacggc accggccttg cgtccgggtt gatgcccgcc ggcctcgggg cgcgccggcc
    15181 aggggagggc cgcgccatag ggattggccg ggtcggtcgc ggcgagcacc agtgcgggcg
    15241 cgtccgccgg cgccgagcgg agggcgtcga tgacgccggc cgtcgcgaac tgggccgctc
    15301 cgagggactc gatgaagtag ccccgccgca gccgtccggt ctcctcggcg gccgagagga
    15361 cgcgatagat gccggcgaac ccgccgggga cctcctccgc ggcgacgatg gagcgggtca
    15421 gcacgccata ccggtcgagg agcacctccg ccgtcgtcag ggtgcgagtc gtcgggtcga
    15481 gatcgacgga cggcaacagc gaccagcggc cgaccgccgt cggcgggccg ctcggcgaca
    15541 tcccaccggc gcgcgccagt ccgctgctgc ggggtatgcc ggcgaggctg ccccggcggc
    15601 cgtagcgcgt cgaccggggg gccggcttgg gccgctgctt atgagccccg cgaccgccgg
    15661 cgagcagtgc gcgaacagga gcgaaggtgt cgcccgtgac ctcgccgcgc cacaccagtt
    15721 cccacagcgc gtcgagcgcg tcccggtcgg agtccacctc ggccgcggac accaggcccc
    15781 ggaagaagtg cgcgccgccc gcggcgaaga cctcgcggag agcgaccgcg tcggccgagc
    15841 cggcaagcga tggacccgag gccgccgaat ctgactcggt tgcagtcttt ttcggatctg
    15901 cgccatccgc ggccgccggg tgcaacgtcc agggggcgct ctcggcgaga tgcaaggaga
    15961 cccacccgtc gtccccgggg agcgagccgt ggccttgcca cagcacgtcg ccggccgaca
    16021 tcaactcgtc gaggaaggcc ggctgataat cggtgacgcg ggccggcagc accagggact
    16081 cgatcgcgct cgcgggcagc agcgccccgc tgagttgttc cacgacacgc agcaccccct
    16141 cgctgccgcg catccggccg ctgccgaccc cctgccaacc ggggaggaag cgggcatact
    16201 gccgggcgtc gaccgcctcg acctcggcac gcaggctggc cagcgaccgc cgacgcaaca
    16261 gccgcaacac ctcggcgtcg cagaactccg tgcccgcccc cgccgggagg aactcgccct
    16321 cggcgatccg cccggccgcc gcgagccggc gcagggcgtc gtgagcgaca gccgtgccga
    16381 tcccgaactc ggccgcgagg tcggtgagca ggaacggggt gtgcgtgcgg gcataccgcg
    16441 cgaccaactc cccgagtgga tccgcaaccg gctcgaggaa ggccgccggc aaccccacgg
    16501 gcaaggccgt gccgagcgca tcccgcaggc gcccggcgtc ctcggccacg gcataccgct
    16561 catcgccggc gatccgcacg gggatgaccc ggcgctgtgc gacgagagcc gtgagggtcg
    16621 catcgaggat ggccgcgagt tcggggtcgg atcgggaggc gaggtcgtcg cggcgcagcg
    16681 gaccgaggat ccggagcagg tcggtcactt cgtcggcatt gcgggcggca cgttcggggg
    16741 tgagccgctg cagctgggcg ctggtggtgg tgatggcccc gacgtcgagc agctcgcgca
    16801 gcgccagcgc ttctcctcgt ccgagcaact cggcgagtag agccgggtcg agggagagcg
    16861 ccgccgcccg ccgctcggca aggggggagt cgccctcgta cagatactgc gcgacgtagc
    16921 cgaacatcag cgaccgggcg aagggcgacg gctgggtcga gtggacgtcg accgttgtga
    16981 tgcggcgggc ggcgaccgag cgcatcaggt ccaccagacc cggcacgtca tagacgtcct
    17041 gcaggcactc gcgcaccgtc tccaggacga tcgggaaatc gggataattc gctgcgacag
    17101 taagcaattg ggccgcccgc tggcgttgct gccacagcgg ttggcgctgg tcggggcggc
    17161 gccgcggcaa cagcagcgcc cgagcggcgc actcgcggaa gcgggaggcg aagagcgccg
    17221 agccgggcag ttgagcggtg acgagggcgt cgacctcgtc ggggtggagg aagagcagct
    17281 cgccgagctc ggcgccggca tcgctgccgt cgtcgaactc gatatcgggc agccgcaaca
    17341 cgatgccgtc gtcggcggcc atcgcctgca cctcgacccc gaaccgctcg cgcatccggg
    17401 cgccgatggc cagcgcccag ggggcgtgga cgggcatccc gaagggggag tgcacggcga
    17461 cccgccagtc gccgatctcg tcgcggaagc gttcgacgac gatcgtggtg tcggagggga
    17521 cgacgccggt ggcttccttc tgctcgccga gataggcgag caggttgtct gctgcccacg
    17581 ggtcgaggcc ggcggcgctc gcgcgggcca gggccttctt cggcgtcaga ccggagagtt
    17641 cgcgcacgaa ggagccgacg gcctggccga gctcgacggg ccggccctgg gtctcgccct
    17701 tccagaaggg cagtttgccg ggctcgccgg gcgcgggggt gacgagcacc tggtcgcggg
    17761 tgatctcctc gatccgccag ctggaggtgc cgagggtgaa gacgtcgccg acgcgggatt
    17821 cgtagaccat ttcctcgtcg agttcgccga cccgccggcc tggcccgtcg ctggcgagga
    17881 agaccccgaa gagcccccgg tcggggatcg tgccgccgct ggtgaccgcg agatgctgcc
    17941 cgcccttgcg ggcgatcacg gtgtccgcca agcgatccca ggtgatccgc ggccgcagtt
    18001 ccgcgaactc ctcactcgga tagcggccgg cgagcatgtc gaggacggat tcgaggacgg
    18061 tgcgcgagag cccggcatag ggcgctgcgc gcctcaggag ggtatgcagt gcgctcacct
    18121 cccaggtgtc catcgccgtc atcgcgacca cctgctgggc gaggacgtcg agtgggttgg
    18181 cggggacggc gagcgcctcg atggcgccgg cgcgcatccg ctcgacgacg acggcggtct
    18241 ggacgaggtc gccgcgatat ttggggaaga agacgccacg ggagatcgcg ccgacctggt
    18301 gcccggcgcg gccgacgcgc tgcagcccgg cggcgacgct cggcggcgat tcgacctgga
    18361 cgacggcatc gaccgagccc atgtcgatgc cgagctcgag agagctcgtc gcgaccacgg
    18421 ccggcagccg gccggccttg agcgcctcct cgatgtcgag gcgctgctct ttgctcaccg
    18481 agccgtggtg agcgcgcgcg agaaccggtg gggcaccggc ggatccgccg gcctgcgcca
    18541 tgacctgcgc gggcgtgcgg gccgggcgcg accgcatacc ctcgacggcc tcgagcgagg
    18601 catcctcggc gaggatgctc gcgtcctgtt cggccgcgag cagccgctcg tcccagatct
    18661 cgttcaggcg tgcggtcagg cgctcggcga ggcggcggga gttggcgaag accagggtgg
    18721 agcgctcgcc ctcgacgaga tcgacgatgc gctcctcgac gtgcggccag atcgaggcgc
    18781 gccgctcggg cccggccgcc gcgccggcga ggtcgccggt cggctcgccg agactgccca
    18841 tgtcgggcac ggggacgacg acctgcaggt cccactcctt ggccgacggt ggcgcgacga
    18901 cctccacggg ccggccgccg gagaggaagc gggcgacctc ctcgatcggg cggaccgttg
    18961 ccgacaggcc gatgcgctgg gcgggcttgg ggagcaaggc atccagccgc tcgaggctga
    19021 ccgccaggtg cgccccgcgc ttggtcgcgg cgacggcgtg cacctcgtcg aggatgacgg
    19081 tctcgacgcc ggccagcgcg tcgcgcgcgt tggaggtgag cagcaggaag agcgactccg
    19141 gggtggtgat gaggatgtcg ctcgggcgct tggcgaaggc gcggcgctcg ttcgccgggg
    19201 tgtcgccgga tcggacggcg acgctcacgt cgggccgggg caggcccagc cggtcggcgg
    19261 cgtggccgat gccgaccagc ggggcccgca ggttgcgttc gacgtcgacg gccagcgcct
    19321 tcatcgggga gatgtagacg acccggcatc gggcgagcgg atcgtccggg accggctctc
    19381 gaaccagccc gtcgatcgcc cacaggaagg ccgacagcgt cttgcccgac ccggtgggcg
    19441 cgacgaccag ggcgtccttg ccgctgctga tcgcctccca cgccccctgc tgcgccggcg
    19501 tgggcgcggg gaagctgccc cggaaccacg cctgggtggc gggggagaag cgatcgagaa
    19561 cgtctgcagt catggccagc ccaactctcg cacgtgccac cgacagggtc gctggtcgag
    19621 gcaacatcgg tccgggtgca caccggctac gagtatcccg tcctggcggg gacgggaggg
    19681 gtgaacgccc tggtcagggg cctgcactcc ccggagcctc ggggcactgc tcgctcgtgt
    19741 gcaccgcgga cgtcgagcgc cgacctgacg cgattggtga cgatgcccgc cgcttctggc
    19801 gtcagtgcct gactcccagg tcggtgaggg caatgtccag ccgggtcgcc agggtgtcgg
    19861 tgagggtgtt cacgaaggtg gcggtgatgt gggagccgga ccggtagacc aggacgttcc
    19921 cgatgaccgg ccagcaggtc ccaccggggc agatcacgtc gttgaggttg accaggcggg
    19981 aggtgtccac catctcgacg gccgccgcga gcgcgcgcgt gccgctgccg ggaccggccg
    20041 gtcgcgagca gtcggacatg tacgacgtgg gatgg
//
LOCUS       DAOPJG010000036        20000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2853_length_20000_cov_7.881775, whole genome shotgun sequence.
ACCESSION   DAOPJG010000036 DAOPJG010000000
VERSION     DAOPJG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 20000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20000
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2853_length_20000_cov_7.881775"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            414..1811
                     /locus_tag="PKU75_05265"
     CDS             414..1811
                     /locus_tag="PKU75_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF80321.1"
                     /translation="MSGAPDIATVSQLRASGHVAKGLREEMRDNLLAKLAAGEDPWPG
                     LHGFEGTVIPQVERALIAGHDIVLLGERGQGKTRLLRTLIGLLDEWTPVIADSELGEH
                     PYEPIAPASIRRAAELGDHLPVAWRHRDERYAEKLATPDTSVADLIGDVDPMKVAEGR
                     HLGDPETIHFGLIPRSHRGIVAVNELPDLAERIQVAMLNVMEERDIQIRGYVLRLPLD
                     VLVVASANPEDYTNRGRIITPLKDRFGAEIRTHYPVELADEIAVIRQEAHLVAHVPDQ
                     LMEILARFTRHLRESSAVDQRSGVSARFSIAAAETVAAAALHRATLQGEAEAVARVVD
                     LAGAVDVLGGKVEFESGEEGRERAILDHLLRTATAETARETFRGIDFRLLVDALTAGA
                     LVMTGDQVSATEFLDALPVLGESELYDEVAARAGAASAGERASALELALEGLFLAKRI
                     GKDSDGQRTVYGERA"
     gene            1820..>3797
                     /locus_tag="PKU75_05270"
     CDS             1820..>3797
                     /locus_tag="PKU75_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF80322.1"
                     /translation="MTRRWDPHRSAYGPYAGGDPLAPPVDLGEALAAIGEDVMAGYSP
                     ERAMREFLRRGGQTQRGLDDLARQVAERRRELLQRHNLDGTMQQVKELLDKAVLAERK
                     QLARDLDDDARFQEMRLDGLPPSPAAAVSSLSDYQWRSSEAREAYDQIKDLLGREMLD
                     QRFAGMKQALEGATDEDRERINEMLGDLNDLLEKHRMGEDTDQDFADFMAKHGDFFPE
                     NPQNVDELIDTLAQRAAAAQRMLNSMTPEQRAELMALSQQAFGSPQLMDSLSRLDANL
                     QMLRPGEDWSGGERFQGDEGLGLGDGTGVFQDLAELDALSEQLGQSYPGARMDDIDLE
                     ALMRQLGEEASVDARTLAQIEKELRESGLLQRSSDGSLRLSPKAMRQLGKSLLRDVAD
                     RMSGRTGQRDLRQAGAAGEPSGASRPWEFGDTETWDVTRTVTNAVQRTVSRGESVRGG
                     IRLELEDIEIVETEARTQAAVALLVDTSFSMAMDGRWLPMKRTALALHQLVSTRFRGD
                     KLQLIAFGRRAQVMDIEELTGLEPLWDKGTNLHHALLLANRHFRKHPSMRPVVLIVTD
                     GEPTSHLESSGRVFFDYPPHPVTIAQTVAELDATARLGAQVTFFRLGDDPGLARFIDQ
                     MARRADGRVVAPEADDLGAAVVGSYLGSRSRGAGG"
     assembly_gap    3798..3897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4042..5268)
                     /locus_tag="PKU75_05275"
     CDS             complement(4042..5268)
                     /locus_tag="PKU75_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF80323.1"
                     /translation="MSARIWHRMPVTRTRHTTLALTALVLGGVGIGTAEFIAMGLLPD
                     TARDLGVSIPAGGASIAAYALGVVVGAPVLAVAGAAWPRRTLLVGLLIVLAVGNALVA
                     LAPNFELLVAARALTGLPHGAFFGVASLAAVDLMPPGMAGRAVGRVMLGIPFANLFGV
                     PAGTWLGQQLGWRTAYGAIAILALLSALLVRVAVPQSFPHPDGAMRRELRALRRVQVW
                     LTLLTAAIGFGGMFAMNSYIAPTFTEVTGQPASIVPVVMFLLGVAGLIGTPLGGRLVD
                     WSVLRTIAIGLGGLALNLALFTVTSRRLIPAIVSVMLCSVCAGLLVVSLQLRLMTVAG
                     DAKNLGAASNHAALNVANALGAWLGGVVLAAGYGYTAPSWVGVALAVCGGLVFALSLA
                     LHQRGGPIRGTSASRG"
     gene            complement(5265..5999)
                     /locus_tag="PKU75_05280"
     CDS             complement(5265..5999)
                     /locus_tag="PKU75_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPF80324.1"
                     /translation="MTGAEVALFGIAAVIIGYSKTSVGGLAVVAVMIFATVMPARAST
                     AAILMVLIVGDLFAIWHYRRNCDVALIRGLLPGVLPGLVLGSVFLAVVDDTLLKRAIG
                     ALLLAMVLLQLLVNSRREQIAERAHGRLPALAAGVGSGFATMTANAAGAVMTLYLVAK
                     QVDKVRFVGTSAWFFFLINCVKVPFSVGLGLLEWTDVWRALALAPAVAVGAWLGVHTI
                     RRISQRGFEVVVLLASAISATVLLLA"
     gene            complement(5996..6691)
                     /locus_tag="PKU75_05285"
     CDS             complement(5996..6691)
                     /locus_tag="PKU75_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF80325.1"
                     /translation="MSISTPVDVRADLIRVAYARVRADGVNSLQARRITKEAGVSTTA
                     VYTHFDGMAGLLLAVAAQAFADFSAAIDAPELTADPLADLLASGIAYRNFAACEPFAY
                     ELLFNRYVSARGPGMRTEFTTAFLPLDDPVGDTAWSHIRRRVEAVIEAGIITEPNADV
                     ATTQLWCGLHGFVCLDIAGHLGPDYQVPVFQGLAFSLLVGLGADRSDVASAIGRAVER
                     VPLAVWTAGGPDA"
     gene            6873..8375
                     /locus_tag="PKU75_05290"
     CDS             6873..8375
                     /locus_tag="PKU75_05290"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HPF80326.1"
                     /translation="MTDNVEFVTAGSGAGPDARSGMSRGKVAAAVVGSMAVVGLAVGG
                     AAAVNVLGGGGAQPEDIVPANAVMFAKIDLDPASGQKIDALRFLRKFPALKDRIGMDD
                     TADLRRKIFEEAQKEGAFKDLSYDADIAPWLGDRFAFAAVPTDGGKDVAAVAILASTD
                     EAKAKAAIAKAGTTGSTCDVVADFVRCAEKPEHLAAFAAGDAPLAKAANFSGDMKDLG
                     EDGIASMWVDVKGAFDLAAAAGATGTDSLPAEQMAKVKEQIGDGRMVAALRFDGPNLE
                     LAGSVKGTKVTGNPGKDAGAIAALPADTIAAVSISGLGEQLTKTWADLEKSFTDLTSS
                     TGGSDPIAEAEQQLGIQLPEDIVKIVGNKTTIAFGGMDTGSGMPKVALKTDGDPAVVQ
                     KLMSKVAEQGQAFGFSSAKGVTLAMDQAYADAVATGSGLDQNATFTAAMPDYAKATQA
                     GFVDIAKVVEQFGADIPADEKANLAPLSAFGFSASQDGGDADFTLRLTTK"
     assembly_gap    8389..8488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8493..9971)
                     /locus_tag="PKU75_05295"
     CDS             complement(8493..9971)
                     /locus_tag="PKU75_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HPF80327.1"
                     /translation="MTQFIAGARVETDGGEPYAVIDPSTGETITEIALGGDEQVDVAV
                     AAARAAYAGWSRATPGERSAVLTRFAAEVDSRAAEFAQVESRQAGKPIRLATEFDVPG
                     TVDNTAFFAGAARHLDGLATAEYSPDHTSSIRREPIGVVGSISPWNYPLQMAAWKILP
                     AVAAGNTIVLKPAENTPLTSVMFAEAATAAGMPPGVINVVVGRGPVAGAHLLRHPEVD
                     MVSFTGSTRVGRTVLEAASATAKRVHLELGGKAPFVVFDDADLEAAIHGAVAGSLINT
                     GQDCTAATRAIVHRSRYADFVAGVADLYAAVRLGPTADPATDLGPLISMAHREKVASM
                     VERARSYARVVTGGAAPGGDLAAGSYYAPTLLADVNPGQEVWTDEIFGPVLTVTAFDS
                     DDEAIALANDTAYGLAASAWTRDVYRAMRASAQIHAGCVWINDHIPIISEMPHGGVKQ
                     SGFGKDMSAYSFEEYTTVKHVMFDRTGDAEKGWHRTIFTLPG"
     gene            10642..>14148
                     /locus_tag="PKU75_05300"
     CDS             10642..>14148
                     /locus_tag="PKU75_05300"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPF80328.1"
                     /translation="MSATTHDQTSDPRARDFRIADRLTATAGQIHLGGLQALVRLPLD
                     QARRDRAAGLRTGGFISGYEGSPLGGYDKELLRLGEQLAAAGVVFRPAVNEELAANAV
                     LGSQLASTRASRTVDGVAAYWYGKAPGLDRATDALRHGNLGGASPLGGVLALVGDDAT
                     AKSSTVPSSSEWALAELGMPVLAPADPQDILRLGLHGIAMSRFSGLWVALKLATNVVD
                     GSATVTLGDPSITLPDNTIDGQPFVHAPHASFLQPGLAQLEESLVGARPELALRYARA
                     NGLDEIAGDQRGKIGIVTAGQTYRNTLEALTLLGVTPDRLGQSGIRVLKLGLVAPLDV
                     AGLLDFAAGLDEIIVVEEKRPLVETAIKSALYGRADAPRVGGKQSPDGQPLLRAHADL
                     PAQLIAQSLGHRIAEVLGSPTIAAATAALATPHTATRPTLPLAPAATRTPYFCSGCPH
                     NRSTATPGGSLVAAGIGCHTLVVFMDPERVGDVIGFTQMGGEGAAWIGMSPFVSEDHL
                     VQNLGDGTYHHSGSLAVRAAVAAGVNVTYRILCNGAVAMTGGQDIVGGMPVPRLAAEL
                     LAEGVAKVAITTEDPSRYAGARLPDGVRVHHRDDLESVLADLAAVPGVTALLNDQECA
                     TELRRKRKRGLAATPNRASFINERVCEGCGDCGAKSNCLSVQPVETEFGRKTRIHQAS
                     CNKDFSCFDGDCPSFIEVTPGSGRPRAARTAGELAVSDLPEPPVTLLDRPIGVRLMGI
                     GGTGIVTTAQVLAVAATNAGLFVRGLDQLGLSQKGGAVISDVRISPESIEGTNAIGPG
                     ECDVYVGADLLVATAPTNLVLTDAGRTWAVVSTTRTPTGSMVADPAVTFPGVDPLMAD
                     LSTRVRPDSVILDARAITEGLFGSDQLTNTFLLGVAVQSGALPLPPAAVEDALSQNGV
                     AVEANHQAFRWGRRYAAAPDAVVAAAAPPPSRSTRAAGTTAYGLVRAAGLPTDGELGE
                     LIARRAEELTAYQDPDYARTYLHHLAQVHRREQAAVPDATSPVTAAVARGLFKLMAYK
                     DEYEVARLSTDPAVAQAIREQFGPDAGYAFRLHPPLLRALGVDSKLTLGTWALPALRG
                     LYAARRLRGSALDPFGHTTVRRAERSLIDEYLRGIVAAVGKLTPDTRDTVVAIAALPD
                     VVRGYEDIKLGNVERFRTQLREQ"
     assembly_gap    14149..14248
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14249..14996)
                     /locus_tag="PKU75_05305"
     CDS             complement(<14249..14996)
                     /locus_tag="PKU75_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HPF80329.1"
                     /translation="MPAIPAEIRAYLDDIVGVFTRSLGPDLTGVWLIGSAAQGVYEHG
                     VSDVDVMVISATRHPREEREAVAGRIVHPALECPTVGLECVWYAVPDLVEMASPVVFQ
                     ANVNGGQVRAPMIALEPDDQPQWWAVLDLAAARQVGIPLLGPPAAQVIPPIPGPRLRR
                     AIEESHAFHDGPDEGSPNRVLNLARLIVLVEDGEWVSKPVGAQRLAQAEPEFAAALDA
                     ALRARAEGHWIDPTLAAGLSRRLADALNASD"
     gene            15055..15489
                     /locus_tag="PKU75_05310"
     CDS             15055..15489
                     /locus_tag="PKU75_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPF80330.1"
                     /translation="MTDREPGSPLRRSAKDLVDEAMTRIETIPVAEVADLLDDPGVLI
                     VDIRDPRELERLGTIPGAFRAPRGMLEFWVDPASPYYKPALDDGRKLILYCGSAWRSA
                     LATAALNDMGRADVAHLEGGFTAWEKAGLPVEGGPVASTVPD"
     gene            15541..15981
                     /locus_tag="PKU75_05315"
     CDS             15541..15981
                     /locus_tag="PKU75_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80331.1"
                     /translation="MSDSYQTRDDNVDAGGETSDVDANPAADIAPPRLAHLVLNGAFL
                     ERHDTTQVQSYVDAVVAVSADSDRDEDGIEADLRERMDAAGIALPDESYRNLARQLHD
                     SAGVAVSTDDGRMLHGDPSLSIANHDPDVRGTDDPEDPDRPFYS"
     gene            complement(15989..19921)
                     /gene="purL"
                     /locus_tag="PKU75_05320"
                     /gene_synonym="purI"
     CDS             complement(15989..19921)
                     /gene="purL"
                     /locus_tag="PKU75_05320"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPF80332.1"
                     /translation="MSAPSDHGLHLLTLEGGSALSDFRARALLSRLQSVAPRIDGVHG
                     RFVHIVASVEPLTEESAATVTRLFTYGQPYAGPTAELGTLVVVTPRLGTISPWSSKAT
                     DIARNCGVDIARVERITEYVLTGPALSEAEWAAAAGVLHDRMTEAVLRDIAAAEALFD
                     AFDAEPMAEVDVLGAGTAALEEANTAYGLALSADEIDYLAEAFTGLGRNPTDVELTMF
                     AQANSEHCRHKIFNADFVIDGAPQPRSLFGMIRHTEEVAGDGTVVAYKDNASIMRGST
                     RTRWLPSGAGTGPSEYAGTEGEVHVLMKVETHNHPTAISPFPGAATGAGGEIRDEGAT
                     GRGSQPKAGLTGFAVSNLRLPGTEEPWETTDAGAPAHIATPLEIMLDGPIGAAAFNNE
                     FGRPGLGGFFRVFEQEVSGISRGYHKPIMSAGGLGTIDAGQTEKVAFPDGTLLVQLGG
                     PGMRIGMGGGAASSMAAGANAAELDFDSVQRGNPEMERRAQEVINHCWSLGPDNPILS
                     IHDVGAGGLSNAFPELVDGAGLGAHFELIAVPQAETGLSPKEIWCNESQERYVLAIAL
                     EDFPRFAQLAERERCPYAVVGVARDHGHLQLSFDTEGEPGAEFAVDMPMEVLLGKPPR
                     MTRDVQRAKWTGDDFDGTGIALRESAYAVLRHPSVASKRFLITIADRSVGGLTHRDQM
                     VGSWQVPVADVAVTLADHVGFDGEAMASGERMPLAAVDAPASGRMAVGEALTNLLAAP
                     ITLRGVKLSCNWMAACGEPGEDAALYDTVHAVAMELCPALGVGVPVGKDSLSMRSKWT
                     DPADGVARQVISPVSLVVTAFANVEDVRGTLTPAAAANQVVLLIDLGGGRHRLGGSIL
                     AQTAARFGTDVPDLDDPKLLVALVDAVNTLREKGIATAYHDRSDGGLWAAVCEMAFAG
                     GVGMDIDLTSLVHGTPGSEIAALFAEELGALVTVPAERTTEAIAILRDHGLSAATHVL
                     GASLREPRVRVSAAGGTVLDEPLGDLATAWDEVSWRISALRDNPVCADEEHAAVAALG
                     ADDGHLVFEPSFDPAEDISAPYLNVGARPKVAILREQGVNSHVETAFAFDRAGFAAYD
                     VHMTDLMTGRADLRDFAGLVAAGGFSYGDTLGAGEGWARSVLFHPELTEAFHEFFHRP
                     DTFGLGICNGCQMFAALAEHIPGAEAWPRFTRNRSEQYEARLSLVEVLDSPSIFFTGM
                     AGSKLPIAVAHGEGFADFSAQGDPGAVQRAMRFVDASGRVATSYPANPNGSPDGLTAV
                     TTGDGRFSAMMPHPERVQRNVQMSWTAGALDAPSPWLRMFRNARVWVG"
BASE COUNT         3183 a         6960 c         6811 g         2746 t
ORIGIN      
        1 cccctcgcgg atcccgaggg gccgcatcgc caacctgcag ggcgacctga ccccccatcc
       61 accggcgcag cggcgagcgt tccaggacaa cctgtggcag gcacggttcc tcctcgccaa
      121 cgctcacaag gcgctcaccc ggcgggacac cgcatacgtc gcgctgtgtt gcagcacagc
      181 cgtcatgatc gccgcgcatg cctggcacgc cgcggccggt cagtgggtga ccaacgagaa
      241 acctggtccc gaatgtcgcc cggctctcac ccggctcgcc ggacttcagc aggcgcgccg
      301 gccgggcgct ggcccacctg ggcgaggatc cggaacagct ggcggcggcc atcgaggaga
      361 tcgagggcat tattaagcag ccgattccca gcagccggag ttagtttggc cccatgagtg
      421 gggcaccgga tatcgccact gtcagccagc tgcgcgccag cgggcatgtc gccaagggtc
      481 tgcgcgagga gatgcgcgac aacctcctcg cgaagctcgc ggccggggag gatccgtggc
      541 ccggtctgca cggcttcgag gggacggtca tcccgcaggt cgagcgggcc ctgatcgccg
      601 ggcacgacat cgtgcttctc ggtgagcggg ggcagggcaa gacgcggctg ttgcgcaccc
      661 tgatcgggtt gctcgacgag tggacgccgg tgatcgccga ctccgaactc ggcgagcatc
      721 cctacgagcc gatcgcgccg gcctcgatcc ggcgcgctgc cgagttgggc gatcacctgc
      781 cggtcgcctg gcgccaccgc gacgagcggt atgccgagaa gctcgccact cccgacacct
      841 cggtcgccga cctgatcggc gacgtcgacc cgatgaaggt cgccgagggc cgtcacctcg
      901 gtgacccgga gaccatccac ttcggcctga tcccccggtc gcaccgcggg atcgtcgccg
      961 tcaacgagct gcccgacctc gccgagcgca tccaggtcgc gatgctgaat gtcatggagg
     1021 agagggatat tcagatccgc ggctatgtgc tccgcctgcc cctggacgtc ctggtcgtcg
     1081 cctccgcgaa cccggaggac tacaccaacc gcggccggat catcactccc ttgaaggacc
     1141 gcttcggcgc ggagatccgc acccactatc ctgtggaact ggcggacgag atcgccgtca
     1201 tccggcagga ggcgcacctc gtcgcccacg tccccgatca gctgatggag atcctcgccc
     1261 gcttcactcg acacctgcgc gagtcctcgg cggtcgacca gcgttccggt gtctcggcgc
     1321 gcttctccat cgcggccgcc gagacggtgg cggccgcggc cctgcaccgc gcgacattgc
     1381 agggcgaggc ggaggccgtc gcccgggtcg tcgacctcgc cggggccgtc gacgtgctcg
     1441 gcggcaaggt cgagttcgag agcggcgaag agggccggga gcgggctatc ctcgaccact
     1501 tgcttcgcac cgcgacggcc gagacggcgc gagaaacgtt ccgcggcatc gacttccggc
     1561 tgctcgtcga cgccctcacc gcgggcgcat tggtcatgac cggtgatcag gtgtcggcga
     1621 ccgagttcct cgacgcactg ccggtgctcg gtgagtcgga gctgtatgac gaggtggccg
     1681 cccgcgccgg ggccgcctcc gccggagagc gggcaagcgc ccttgagttg gccctggaag
     1741 gcctcttcct cgccaagcgg atcggcaagg acagcgacgg gcagcgcacg gtttacggcg
     1801 aaagggctta ggggacaagg tgactcggcg atgggatccg caccggtcgg catacggacc
     1861 gtatgccggc ggcgacccgc tcgcgccgcc ggtcgacctc ggcgaggcgc tcgcggcgat
     1921 cggcgaggac gtcatggccg gctactcccc cgagcgggcg atgcgcgagt tcctgcgccg
     1981 cggcggacag acccagcgcg ggctggacga cctcgcgcgg caggtcgccg agcgacgccg
     2041 cgagttgctg cagcggcaca acctcgacgg cacgatgcag caggtcaagg aactcctcga
     2101 caaagccgtt ctcgcagaac gcaagcagct tgcgcgtgac ctcgacgacg acgcgcgctt
     2161 ccaggagatg cgcctcgacg ggctgcctcc gtcgcccgcg gcagcggtct cgtcgttgag
     2221 tgactaccag tggcgcagca gcgaggcgcg cgaggcctac gaccagatca aggacctgct
     2281 cgggcgggaa atgctggacc agcgcttcgc cggtatgaag caagccctcg aaggcgccac
     2341 cgacgaggac cgcgagcgga tcaacgagat gctcggcgac ctcaacgacc tcctcgagaa
     2401 gcaccggatg ggcgaggaca ccgaccagga cttcgccgac ttcatggcca agcacggcga
     2461 cttcttcccg gagaacccac agaatgtcga tgaactgatc gacactcttg cccagcgcgc
     2521 ggcggccgcc caacggatgc tcaattcgat gacgccggag cagcgggccg agctgatggc
     2581 gctgtcgcag caggcttttg ggtcgccgca gctcatggat tcgctcagcc ggctcgacgc
     2641 caacctgcag atgttgcgac ctggtgagga ctggagcggc ggcgagcgct tccagggcga
     2701 cgagggactc ggcctcggcg acggcacggg cgtcttccag gacctcgccg aactcgatgc
     2761 gctgagtgag caactgggac agtcctatcc gggcgcccgc atggacgaca tcgacctcga
     2821 ggccctgatg cgccagctgg gcgaggaggc ctcggtcgac gcccgcaccc tggcccagat
     2881 cgagaaggag ctgcgcgaga gcggtttgct gcaacgctca tccgacggct cgcttcgcct
     2941 ctcccccaag gcgatgcgac aactcggcaa gtccttgctg cgagacgtgg cggaccgcat
     3001 gtccgggcgc acgggacagc gggacctgcg ccaggccggg gccgccggcg agcccagcgg
     3061 ggccagccga ccgtgggagt tcggcgacac cgagacctgg gacgtcaccc gcacggtgac
     3121 caacgcggtc cagcgcacgg tgagccgggg cgagtccgtg cgtggcggca tacggctgga
     3181 actcgaggac atcgagatcg tcgagaccga ggcgcgcacc caagccgccg tggcgctcct
     3241 cgtggacacg agcttctcga tggcgatgga cggccggtgg ctgccgatga agcgcacagc
     3301 gctggctctg caccagctcg tctccacccg gttccgcggc gacaagctgc aattgatcgc
     3361 cttcggccgg cgcgcccagg tcatggacat cgaggagctg accggcctgg aacccttgtg
     3421 ggacaaggga acaaacctcc accatgcgct gctgctcgcc aaccggcact tccgcaagca
     3481 cccgagcatg cgcccggtcg tcctcatcgt caccgacggt gaaccgacct cgcacctgga
     3541 gagcagcggg cgggtgttct tcgactaccc gccgcacccg gtgacgatcg cgcagaccgt
     3601 cgccgaactg gacgccaccg cccggctcgg cgcccaggtg accttctttc gcctcggcga
     3661 cgaccccggc ctcgcgcgtt tcatcgatca gatggcccgc cgggccgacg gccgtgtcgt
     3721 cgccccggag gccgacgacc tcggtgccgc cgtcgtcggc tcctatctcg gctcccgcag
     3781 ccgcggcgcc ggcggttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc
     3901 ggcggttttg gtggcggcgg ctacggcggc tcgacctggc gttcggcctg gacggacttc
     3961 tgaccgcccc gcccccgccg agcaacctct gctagcagaa gttggtgaaa tcagcacagc
     4021 tagcacggtg tgcggatttg actaacctct gctagcagag gttcctcgga tgggcccgcc
     4081 gcgctggtgg agggcgaggg agagggcgaa gacgaggccg ccgcatacgg ccagggcgac
     4141 gccgacccag gagggggcgg tgtatccgta gccggcggcc agcaccacgc cgccgagcca
     4201 ggcgccgagg gcgttggcga cattgagcgc ggcgtgattg cttgctgccc caaggttttt
     4261 cgcgtccccg gcgacggtca tcaaccgcaa ctgcaggctg acgacgagga ggccggcgca
     4321 caccgagcag agcatgacgc tgacgatggc ggggatcagc cggcgggagg tcacggtgaa
     4381 cagcgcgaga ttcagcgcca ggccgccgag cccgatcgcg atggtccgca gcaccgacca
     4441 gtcgacgagc cgcccaccga gcggcgtgcc gatcaggccg gcgacgccga gcagaaacat
     4501 cacgaccggc acgatagacg ccggctgacc ggtcacctcg gtgaaggtcg gcgcgatata
     4561 ggagttcatc gcgaacatcc cgccgaagcc gatggccgca gtcagcaagg tgagccacac
     4621 ctgcacccgg cgcagcgccc gcagctcgcg gcgcatcgcg ccgtcggggt gcggaaacga
     4681 ctggggcaca gcgactctca caaggagagc ggagagcagg gccaggatcg cgattgctcc
     4741 gtatgcggtg cgccagccga gttgttgccc cagccaggtg cccgccggga ccccgaagag
     4801 gttggcgaac gggatgccca gcatgacccg cccgaccgcg cggccggcca tcccgggggg
     4861 catcagatcg acggcggcga gggaggcgac gccgaagaag gcgccgtgcg gcaggcccgt
     4921 cagagcccgc gcggcgacga gcagctcgaa gttcggagcc agcgccacga gggcattgcc
     4981 gacggcgagc acgatcagca gacccacgag cagggtgcgg cgcggccagg ccgcaccggc
     5041 gaccgccagc accggcgcgc cgacgacgac accgagggca taggcggcga tggacgcccc
     5101 acccgcgggg atgctcaccc ccagatcccg cgcggtgtcc ggcaacaggc ccatcgcgat
     5161 gaactcggcg gtcccgatcc cgacgccgcc gagcacgagt gcggtcaggg cgagggtggt
     5221 atgccgggtg cgcgtcaccg gcatacggtg ccagatccgc gcgctcacgc caggagcagc
     5281 acagtggccg agatggcgga ggccagcagg acgacgactt cgaagccgcg ctgggagatc
     5341 cgccggatgg tgtgcacccc cagccacgcg cccacggcga cggccggggc gagggcgagc
     5401 gcgcgccata cgtcggtcca ctcgagcaga cccagcccga cgctgaaggg gaccttgacg
     5461 caattgatga ggaagaagaa ccacgcgctc gtgccgacga agcgcacctt gtcgacctgc
     5521 ttggccacga gatagagggt catcacggca ccggcggcat tggcggtcat cgtcgcgaag
     5581 cccgacccca ctccggcggc gagcgcgggg aggcgtccgt gggcgcgctc ggcgatctgt
     5641 tcgcggcggg agttgacgag aagttgcagc agcaccatgg cgagcaacaa ggcaccgatc
     5701 gcgcgcttca ggagcgtgtc gtcgacgacg gcgaggaaga ccgacccgag gaccaacccg
     5761 gggagcacgc cggggagcag tccgcggatc agggcgacat cgcagttgcg gcggtagtgc
     5821 cagatggcga acaggtcacc gacgatcagg accatcaaga tggcggccgt cgaggcgcgg
     5881 gccggcatga ccgtggcgaa gatcatgacc gcgacgaccg cgaggccacc gaccgaggtc
     5941 ttggagtatc cgatgatcac ggcagcgatg ccgaaaaggg cgacctctgc gccggtcacg
     6001 cgtcgggccc gccggccgtc cacacggcga gggggacccg ttcgacagcg cggccgatgg
     6061 cactggcgac atcggaccgg tccgcgccca gcccgacgag cagagagaag gccaggcctt
     6121 ggaagaccgg cacttggtag tcgggtccga gatgaccggc gatgtcgagg cagacgaaac
     6181 cgtgcagtcc gcaccagagt tgcgtcgtgg ccacatcggc gttgggctcg gtgatgatgc
     6241 ccgcctcgat gacggcctcg acgcgccgcc ggatgtgcga ccaggccgtg tcgccgaccg
     6301 ggtcgtccag cgggaggaag gcggtggtga actcggtgcg catcccgggc ccgcgggcgc
     6361 tgacatagcg gttgaagaga agctcgtagg cgaacggctc gcacgcggcg aagttgcggt
     6421 aggcgatgcc gctggcgagc aggtccgcga gcgggtccgc ggtcaactcc ggcgcgtcga
     6481 tggcggcgct gaagtcggcg aacgcctgcg cggcgacggc caggagcagc ccggccatgc
     6541 cgtcgaagtg ggtgtagacg gcggtggtcg atactcccgc ttccttggtg atgcgccggg
     6601 cctgcaagga gttcacgccg tcggcgcgga cccgggcgta ggcgacgcgg atcagatccg
     6661 cacgcacgtc gacgggtgtg gagatgctca cctaagcctg gcctctgcgc tcgtgtcgac
     6721 gccccccaga gcgtcgaact acgccgccat cctacggcgc aattcagggg cctccggggg
     6781 aatgacccga cttggggaac cacccgtgac gcgctagcgt cgatgcgtac gagtgccgcg
     6841 accaagcggc cacgtgaggg gagagcagtc agatgacgga caacgtcgaa ttcgtgaccg
     6901 caggctcggg tgcgggaccc gacgcccgca gcggtatgtc gcggggcaag gttgccgccg
     6961 ctgtcgtggg gagcatggcc gtggtcgggt tggctgtcgg cggcgccgcc gccgtcaacg
     7021 tgctgggcgg cgggggtgcg cagccggagg acatcgtgcc ggccaacgcc gtgatgttcg
     7081 ccaagatcga tctggacccg gcgagcgggc agaagatcga cgcgctgcgg ttcctgcgca
     7141 agttcccggc gctcaaggac aggatcggga tggacgacac cgccgacctg cgccgaaaga
     7201 tcttcgaaga ggcccagaag gagggcgcct tcaaggatct gtcgtatgac gcggacatcg
     7261 ccccgtggct cggcgaccgg ttcgccttcg ctgcggtgcc gaccgatggc ggcaaggatg
     7321 tcgcggccgt cgcgatcctt gcctcgacgg acgaggcgaa ggccaaggcc gccatcgcca
     7381 aggccgggac gaccggatcc acctgtgatg tcgtggccga cttcgtccgc tgcgccgaaa
     7441 agcccgagca cctggccgct ttcgccgcgg gcgacgcccc gctggccaag gcggcgaact
     7501 tctccgggga catgaaggac ctcggcgagg acggcatcgc gagcatgtgg gtcgacgtca
     7561 agggcgcctt cgacctggcc gcggccgcgg gcgcgaccgg gaccgactcc ctgcccgccg
     7621 agcagatggc caaggtcaag gagcagatcg gcgacggccg catggttgcc gcgttgcgct
     7681 tcgacggccc caacctcgag ctggccggca gcgtcaaggg caccaaggtg acgggcaacc
     7741 cgggcaagga cgccggcgcc atcgcggcgc tgccggcgga cacgatcgcc gccgtgagca
     7801 tctccggcct gggcgaacag ctcaccaaga cctgggctga cctcgagaag agcttcaccg
     7861 acctcaccag ctcgaccggc ggctccgacc cgatcgccga ggcggagcag cagctgggca
     7921 tccaactgcc agaggacatc gtcaagatcg tcggcaacaa gacgaccatc gccttcggcg
     7981 gcatggacac cggctctggc atgcccaagg tcgcgctgaa gaccgacggc gacccggcag
     8041 tcgtgcagaa gctgatgagc aaggtcgccg aacaggggca ggccttcggc ttctcctcgg
     8101 ccaagggcgt cacgctggcg atggatcagg cgtatgccga tgccgtcgcc accggttccg
     8161 gcctggacca gaacgccacc ttcaccgccg cgatgcccga ctacgccaag gccacccagg
     8221 ccggcttcgt cgacatcgcc aaggtcgtcg agcagttcgg cgccgacatc ccggccgacg
     8281 agaaggccaa cctggcgccg ttgtcggcct tcgggttcag cgcctcccag gacggcggcg
     8341 acgccgactt caccctccgc ctgaccacca agtagcccgc gggccaagnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg tttcagcccg ggagggtgaa tatcgtgcgg
     8521 tgccaaccct tttcggcgtc gccggtgcgg tcgaacatca cgtgcttgac ggtcgtgtac
     8581 tcctcgaacg agtaggccga catgtccttg ccgaagccgg actgcttcac cccgccgtgc
     8641 ggcatctcgc tgatgatcgg aatgtggtcg ttgatccaca cgcaaccggc gtggatctgc
     8701 gcgctggcgc gcatggcccg atagacgtcg cgggtccagg cggaggccgc gaggccgtat
     8761 gcggtgtcat tggccagggc gatcgcctcg tcgtcgctgt cgaaggccgt tacggtcagg
     8821 acggggccga agatctcgtc ggtccagact tcctgaccgg ggttgacatc ggcgagcagg
     8881 gtgggggcgt aataggaccc ggccgccagg tccccgccgg gcgccgcacc gccggtgacc
     8941 acccgggcat acgagcgggc gcgctcgacc atgctggcga ccttctcgcg atgggccatc
     9001 gagatcagcg gtccgaggtc ggtggccggg tcggcggtcg ggccgagccg gaccgcggca
     9061 tacagatccg ccactcccgc cacgaaatcg gcatagcgcg agcggtgcac gatggcgcgg
     9121 gtcgcggccg tgcagtcctg gccggtgttg atcaggctgc ccgcgaccgc gccgtggatc
     9181 gcggcctcca ggtcggcgtc gtcgaacacg acgaagggcg ctttgccgcc gagctcgagg
     9241 tggacgcgtt tcgcggtggc cgaggcggcc tcgaggacgg tgcgcccgac gcgcgtggag
     9301 ccggtgaagg agaccatgtc gacctcgggg tggcgcagca ggtgggcgcc ggcgaccggc
     9361 ccgcggccga cgaccacgtt gatcacgccg ggcggcatcc cggccgctgt cgcggcttcg
     9421 gcgaacatca ccgacgtcaa gggcgtgttc tccgccggtt tgaggacgat cgtgttgccg
     9481 gccgcgaccg cgggcaggat cttccaggca gccatctgca gggggtagtt ccacggcgag
     9541 atggacccga cgaccccgat cggttcgcgg cggatgctgc tggtgtgatc gggtgagtat
     9601 tcggcggtgg ccaggccgtc gagatgccgg gcggcgcccg cgaagaaggc ggtattgtcc
     9661 accgtccccg gcacgtcgaa ctcggtcgcg agccggatcg gtttgcccgc ctgccgggac
     9721 tcgacctgcg cgaattccgc tgcgcgcgag tcgacttcgg ccgcgaaacg ggtgagcacg
     9781 gccgaccgct ccccgggggt cgcccgggac cagccggcat acgcagcccg cgcggcggcg
     9841 acggcgacat cgacctgctc gtcgcccccg agcgcgatct cggtgatcgt ctcgcccgtc
     9901 gacgggtcga tgacggcata cggctcgccg ccgtcggtct cgacgcgggc gccggcgatg
     9961 aactgggtca tggctcgacc ctactcacgg cgtcagccga actgggccac gtcgtcgaat
    10021 ccggctagcc caggctggcc ccgggctcgg ctccagcagg cagccggaac ggcgggcata
    10081 cctcgcaccc gcactccacg gcgcccgcgc gccggaatcc gaccttgtcc agcacgcggg
    10141 tgcaggcgtg gtttcccggg gagacgtaag cccacagggg cacgtggtca gcgaactgtc
    10201 gatgccaacc gaccagcgcg gtcgcgacct cggtcgccag gccctgaccc aacgatcccg
    10261 gcgaatggtg tagcccagct ggcgcccgcc ccggaacgcg gcgaacccgg ctcgaccgac
    10321 cagcaaggcc cgtggtcggc aacgcggcac ctcgtgaagc ccgctcgatc ctgctcggtg
    10381 atgtacccat cgatcagggc cgcgacttcg gcgtgggtct ccggccggcg ccatgacgaa
    10441 cgaaacgcat cgtctcgggg tccgagtgga tcgtgagcag tgcgtccacg tcgtcgggct
    10501 cccacgtcgt gaccacaagg cggcagatac tgggcaccgt tgtgcgacac acaagggcca
    10561 tcatcccgga ctgccgatgc cccgtccacc actggggaaa atcgctgtag tgtccgtgct
    10621 ctggcggcca gaaggggagc aatgagcgcg acgacccacg accagacctc ggacccgcgg
    10681 gcgcgggact tccggatcgc cgaccggctg accgcgaccg caggccagat ccacctcggc
    10741 ggactgcagg cgctggtgcg cctgcccttg gaccaggcgc ggcgggaccg ggcggccggg
    10801 ctgcggaccg gcggtttcat cagcgggtat gaggggtcac cactcggcgg ctacgacaag
    10861 gaactgctgc ggctcggcga gcagctggcg gccgccggcg tcgtcttccg gcccgccgtc
    10921 aacgaggaac tggccgccaa cgccgtgctc ggctcgcagc tcgcgagcac ccgggcgagc
    10981 cgcaccgtcg acggggtggc cgcctattgg tacggcaagg cgccgggcct cgaccgggcc
    11041 accgacgccc tgcggcacgg caacctcggc ggcgcctccc cgctcggtgg ggtgctcgcg
    11101 ctcgtcggcg acgacgcgac cgccaagagc tcgacggtgc cgagcagctc ggagtgggcg
    11161 ctcgccgaac tgggtatgcc ggtgctcgcc cccgccgacc cgcaggacat tctgcgcctg
    11221 ggcctgcacg gcatcgcgat gtcgcgcttc agcggattgt gggtggcgct gaaactcgcc
    11281 accaatgtcg tagacggcag cgcgaccgtg accctgggcg acccctcgat cacgctgccg
    11341 gacaacacga tcgacggcca gccgttcgtg cacgccccgc acgccagctt cctgcagccc
    11401 gggttggcgc aactggagga gtcgctcgtc ggcgcacggc ccgagctggc gctgcgctac
    11461 gcccgcgcca acggcctgga cgagatcgcc ggcgaccagc gcgggaagat cgggatcgtc
    11521 accgccggcc agacctatcg caataccctc gaggccctga ccctcctcgg cgtcacaccg
    11581 gatcgcctgg gccagagcgg gattcgcgtc ctgaaactcg gactggtcgc gcctctcgat
    11641 gtcgccgggc tgctcgactt cgcggccggc ctcgacgaga tcatcgtcgt cgaggagaag
    11701 cggcccctgg tcgagaccgc catcaagtcg gccctctacg gccgggccga cgcgccgcgc
    11761 gtcggcggca agcagtctcc cgacgggcag ccgctgctgc gggcgcacgc cgacctgccg
    11821 gctcagctga tcgcccaaag cctcgggcac cggatcgccg aggtgctcgg ctcgccgacg
    11881 atcgcggccg cgaccgcggc gctggccaca ccgcataccg cgacccggcc caccctcccg
    11941 ctggcccccg cggcgacacg cacgccctat ttctgctccg gctgcccaca caaccgctcc
    12001 accgccacgc ccggcggcag cctcgtcgcc gccggcatcg gctgccacac cctcgtcgtc
    12061 ttcatggacc ccgagcgggt cggcgatgtc atcggcttca cccagatggg cggcgagggc
    12121 gcggcgtgga tcgggatgtc tcccttcgtc tccgaagacc acctggtcca aaatctcggc
    12181 gacggcacct atcaccactc cggcagcctc gccgtccgcg cggccgtcgc cgcgggcgtc
    12241 aatgtcacct atcggatcct gtgcaatggc gcggtcgcga tgaccggtgg ccaggacatc
    12301 gtcggcggta tgccggtgcc ccgcctcgcc gccgaactgc tcgccgaggg cgtcgcgaag
    12361 gtcgccatca ccacggagga cccgagccga tatgccgggg cacgcctgcc ggacggtgtg
    12421 cgcgtccacc accgcgacga cctggagtcg gtgctcgccg acctcgctgc cgtccccggc
    12481 gtcaccgccc tgctgaacga ccaggagtgc gcaaccgagt tgcggcgcaa gcgaaagcgc
    12541 ggcctggcgg cgacgccgaa ccgcgcgtct ttcatcaacg aacgggtctg cgaagggtgc
    12601 ggcgattgcg gcgccaagtc caactgcctg tcggtgcagc cggtcgagac cgagttcggc
    12661 cgcaagaccc gcatccacca ggccagctgc aacaaggact tctcctgctt cgacggggac
    12721 tgcccgtcct tcatcgaggt gacccccggg agcggccgcc cccgcgcggc ccggaccgcg
    12781 ggggaacttg ccgtgagcga cttgcccgag ccacccgtga ccctcctgga ccggccgatc
    12841 ggcgtgcgcc tcatgggaat cggcggcacc ggaatcgtca ccacggcaca ggtcctcgcc
    12901 gtcgccgcga cgaatgccgg cctcttcgtg cgcggcctgg atcagctcgg cctgtcgcag
    12961 aagggcggcg cggtgatctc cgatgtgcgc atctcccccg agtcgatcga gggcaccaat
    13021 gccatcggcc cgggcgaatg cgatgtatat gtcggcgccg acctgctcgt cgccaccgcg
    13081 ccgaccaatc ttgtgctcac cgatgccggg cgcacctggg cggtcgtctc caccacgcgc
    13141 accccgaccg gatcgatggt cgccgacccc gccgtcacct tccccggcgt cgacccgctc
    13201 atggcagacc tgagcacccg ggtccgcccc gacagcgtca tcctcgatgc ccgcgcgatc
    13261 accgagggcc tcttcggcag cgatcagctg accaacacct tcctcctcgg tgtcgccgtg
    13321 cagtccggcg ccctgcccct cccgccggcc gctgtcgaag atgccttgtc gcagaacggc
    13381 gttgccgtcg aggccaacca ccaggccttc cggtggggtc gacggtatgc cgcggctccc
    13441 gacgccgtgg tggccgccgc agcgcctccg ccaagccgct ccacccgtgc ggcggggacc
    13501 accgcatacg ggctcgtccg cgcagcgggc ctgccgaccg acggcgagct gggcgaactc
    13561 atcgctcggc gagccgagga actgaccgcc tatcaggacc cggattatgc gcgaacctat
    13621 ctgcaccacc tcgcgcaggt gcaccggcgc gagcaggccg ccgtcccgga cgccacgagc
    13681 ccggtgacgg ccgcggtggc acgtgggctg ttcaagctga tggcctacaa ggatgagtat
    13741 gaggtcgccc gcctgtcgac ggatcccgct gtcgcccagg cgattcgcga gcagttcggc
    13801 cccgacgcgg gctatgcctt ccggctgcac ccgccgctac tgcgcgcgct cggtgtcgat
    13861 tccaagctca ccctcggcac ctgggccctc cctgcgctgc gcggcctgta cgcggcgcgc
    13921 cgcctgcgcg gcagcgcact ggatcctttc gggcacacca cggttcggcg cgccgagcgg
    13981 tcgctcatcg acgagtatct gcggggaatc gttgcggcag ttgggaaatt gaccccggac
    14041 actcgggaca cggtcgtcgc catcgccgcc ctgcccgacg tggtccgcgg ctatgaggac
    14101 atcaagctgg gcaatgtcga gcggttccgc acccaactgc gagagcagnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg tcgctcgcgt tcagcgcgtc cgcgagacga
    14281 cggctgagcc ctgccgcgag agtggggtcg atccagtggc cctccgcgcg cgcccggagc
    14341 gcggcgtcga gcgccgccgc gaattccggc tccgcctgcg caagccgctg ggcgccaaca
    14401 ggtttcgaga cccactcccc atcctccacg agcacgatga ggcgtgccag gttgaggacc
    14461 cggttcgggc tgccctcatc cggcccgtcg tggaaggcgt gcgactcctc gatcgcgcgc
    14521 cgcagccgcg gcccgggaat cggcggaatg acctgtgccg caggcggtcc aagcaacggt
    14581 atgccgacct gccgggcagc cgccagatcg agcaccgccc accattgcgg ctggtcgtcc
    14641 ggctccaagg cgatcatcgg cgcccggacc tgaccgccgt tgacattggc ctggaacacg
    14701 accggcgagg ccatttcgac aaggtcgggc acggcatacc aaacgcattc gaggccgacc
    14761 gtggggcact cgagcgctgg atgcacgatg cgcccggcca cggcttcacg ctcctcccga
    14821 ggatgtcgcg tggctgagat gaccatgacg tcgacatcgc tgacgccgtg ctcgtagacg
    14881 ccctgcgctg cactgccgat gagccatacc cccgtcaggt cgggtccgag cgaccgcgtg
    14941 aacaccccca cgatgtcgtc gaggtaggca cggatctcgg ccggaatcgc tggcatggac
    15001 acatcgtctc gccgcagcgg cgtgttgtgc cgccgaacac gcaacaatcc agccatgacc
    15061 gaccgcgaac ccggaagccc gctgcggcgc agcgccaagg acctggtcga cgaggcgatg
    15121 acccgcatcg agaccatccc cgtcgcggag gtcgcggatc tgctcgacga ccctggcgtc
    15181 ctcatcgtcg acatccgcga cccccgcgag ctggagcgac tcgggaccat ccccggcgcg
    15241 ttccgcgcgc cccgcgggat gctggagttc tgggtcgatc cggccagccc ttactacaag
    15301 cccgcgctcg acgacggccg caagctgatc ctctactgcg ggtcggcctg gcgttcggca
    15361 ctcgcgacgg ccgccctcaa tgacatgggg cgggccgatg tcgcccacct cgagggtggg
    15421 ttcaccgcgt gggagaaggc aggcctcccg gtcgagggcg gcccggtcgc atcgaccgtt
    15481 ccggattgac cggacgctga cgggcagggc gcgcggtcgg ctctcctaca gttggaggct
    15541 atgagtgata gctaccagac tcgcgacgac aacgtcgacg ccggcggcga gacctccgac
    15601 gtcgacgcca acccggcggc cgacatcgcc cctccgcggc tcgcccacct cgtgctcaat
    15661 ggggcctttc tggagcggca cgacacgacg caagtgcagt catatgtcga tgccgtcgtg
    15721 gccgtgagcg cggactccga tcgcgacgag gacggcatcg aggcggacct gcgcgagcgg
    15781 atggacgccg ccggcatcgc cctgccggac gagtcctacc ggaatctggc ccggcagttg
    15841 catgacagcg ccggggtcgc ggtcagcacc gatgacggac gcatgctgca cggcgacccg
    15901 tcgctgtcga tcgcgaacca cgacccggac gtgcgcggca ccgacgaccc cgaggacccc
    15961 gaccgtccgt tctactcctg atcgccgctc aacccaccca gaccctggcg ttgcggaaca
    16021 tccgcaacca cgggctgggc gcgtcgagtg cgccagcggt ccaggacatc tggacattgc
    16081 gctgcacccg ctcggggtgc ggcatcatgg cgctgaaccg gccgtcgccg gtggtgaccg
    16141 cggtcaatcc gtccggggac ccgttcgggt tcgccggata gctggtggca acccggcccg
    16201 acgcgtccac gaaacgcatc gcacgctgga ccgcccctgg gtcgccttgc gccgagaagt
    16261 cggcgaagcc ctcgccgtgt gcgacggcaa tcggcagctt cgagccggcc atcccggtga
    16321 agaagatcga aggtgagtcc agcacctcca cgagggagag gcgggcctca tactgctcgg
    16381 agcggttgcg ggtgaagcgg ggccacgcct cggcgcccgg aatgtgctcg gccagggctg
    16441 cgaacatctg gcagccgttg cagatgccca ggccgaaggt gtcgggccgg tggaagaact
    16501 cgtggaaggc ctcggtcagc tccggatgga agaggaccga gcgtgcccag ccctcgccgg
    16561 cgccgagcgt gtcgccatag gagaacccgc cggccgcgac gagccccgcg aaatctcgaa
    16621 gatccgctcg tccggtcatc agatcggtca tgtggacgtc gtatgccgcg aacccggccc
    16681 gatcgaacgc gaaggccgtc tcgacatggg agttcacccc ttgctcccgc aggatggcca
    16741 ccttcgggcg ggcaccgacg ttgaggtatg gcgccgaaat gtcctcggcc gggtcgaaac
    16801 tcggctcgaa cacgagatgc ccgtcatcgg cccccagcgc cgcgaccgcc gcatgctcct
    16861 cgtcggcgca cacgggattg tcccgaaggg cgctgatccg ccaggacacc tcgtcccacg
    16921 ccgtagcgag gtcgccgagc ggctcgtcga gcacggtgcc gcccgccgcc gagacgcgca
    16981 ctcgcggctc gcgcaggctg gccccgagga catgggtcgc cgcgctgaga ccgtggtcgc
    17041 gcaggatggc gatggcctcg gttgtccgct cggccggcac cgtgacgagc gcgccgagtt
    17101 cctcggcgaa gagcgcggcg atctcgctgc ccggcgtgcc gtgcaccagc gaggtcagat
    17161 cgatgtccat accgacaccc cccgcgaacg ccatctcgca cacggccgcc caaagccccc
    17221 cgtcggaccg gtcgtggtat gccgtggcaa tccccttctc ccgcaacgta ttgaccgcgt
    17281 cgaccaaggc gacgagcagt ttcggatcat cgaggtcggg cacgtccgtg ccgaaccgtg
    17341 ccgccgtctg cgcgaggatc gagccgccga gccgatgccg gccgccgccg aggtcgatca
    17401 ggagcaccac ctggttggcc gccgccgcag gggtgagggt gccacggaca tcctcgacgt
    17461 tcgcgaaggc cgtgacgacc agtgagacgg gggaaatgac ttgtcgggca acgccgtccg
    17521 ccggatcggt ccacttgctg cgcatcgaca gcgagtcctt accgacgggc acgccgaccc
    17581 ccagagccgg acacaactcc atcgccacgg cgtggacggt gtcatagagt gccgcgtctt
    17641 cccccggctc accgcacgcg gccatccagt tgcacgacaa cttcacgccc cgcagggtga
    17701 tcggcgcagc caacaggttg gtcagcgcct cacccaccgc catccgccca gaggcggggg
    17761 catcgacggc ggcgagcggc atccgctctc cggacgccat cgcctcaccg tcgaacccga
    17821 cgtgatcggc gagggtgacc gcgacgtcgg cgaccggcac ctgccacgac ccgaccatct
    17881 ggtcccggtg ggtcaggccg ccgacggacc ggtcggcgat ggtgatcagg aagcgtttgc
    17941 tggcgacgct cgggtgacgc agcacggcat acgcgctctc gcgcagggcg atcccggtgc
    18001 cgtcgaagtc gtcgcccgtc catttggccc gttgcacgtc ccgcgtcatc cgcggcggtt
    18061 tgccgagcag cacctccatc ggcatgtcga cggcgaactc ggcgcccggt tctccctcgg
    18121 tgtcgaagga cagttgcaga tgaccgtgat cgcgggcgac tccgacgacg gcatacgggc
    18181 agcgctcccg ttccgccagc tgagcaaaac gcgggaagtc ctctagagcg atcgccagga
    18241 cataccgctc ctgactctcg ttgcaccaga tctctttggg cgacaagccc gtctcggcct
    18301 ggggcacggc gatgagttcg aagtgcgcgc cgagccctgc gccgtccacg agttcgggga
    18361 aggcgttcga caggccgccg gcgcccacgt cgtgaatgga caggatgggg ttgtccggac
    18421 cgagggacca gcagtggttg atgacctcct gggcgcgccg ctccatctcc gggttgccgc
    18481 gctggaccga atcgaagtcc agctcagcgg cattcgcgcc ggcggccatc gacgacgcgg
    18541 cgccgccacc catgccgatc cgcattcccg ggccgcccag ctgcacgagc agggtgccgt
    18601 cggggaaggc gaccttctcc gtctggccgg catcgatggt gccgagccca cccgcgctca
    18661 taatcggctt gtggtagccg cggctgatcc cggagacctc ttgttcgaag acccggaaga
    18721 agccgccgag accgggccgg ccgaactcgt tgttgaacgc cgccgcaccg ataggaccgt
    18781 ccagcatgat ctcgagcggt gtcgcgatat gtgccggagc gcccgcatcg gttgtctccc
    18841 aaggctcttc ggtgccgggc aggcgcaggt tggacacggc gaagccggtg agcccggcct
    18901 tgggttgcga cccccgtccg gtcgcccctt cgtcgcggat ctcgccgccc gccccggtgg
    18961 ccgcgcccgg gaagggcgag atcgccgtcg ggtggttgtg ggtctcgacc ttcatcagga
    19021 catggacctc accttcggtg ccggcgtact cactcggccc ggtgccggca cccgacggca
    19081 gccaccgggt gcgcgtggat ccgcgcatga tggaggcatt gtccttgtag gcgacgaccg
    19141 tgccgtcacc agcgacctct tcggtgtgcc ggatcatccc gaagagcgaa cgcggctgcg
    19201 gggcaccgtc gatgacgaag tcggcattga agatcttgtg ccggcagtgc tcggagttgg
    19261 cctgcgcgaa catcgtcaac tcgacatcgg tggggttgcg ccccaacccg gtgaacgcct
    19321 cggcgaggta gtcgatctca tccgccgaca gggccaggcc gtatgccgtg ttggcctcct
    19381 caagagcagc agtccccgcg ccgagcacgt cgacctcggc catcggctct gcgtcgaatg
    19441 cgtcgaacag ggcctcggcg gcggcgatgt cacgcaggac ggcctcggtc atccggtcgt
    19501 gcaggacacc agcggccgcg gcccactcgg cctcgctcaa cgccggtccg gtcagaacgt
    19561 attcggtgat ccgctccacc cgggcgatgt cgacaccaca gttgcgggcg atgtcggtcg
    19621 ccttgctcga ccacggcgag atcgtgccca gccggggtgt gacgacgacg agtgtgccca
    19681 gctcggcggt ggggccggca tacggctgcc cgtaggtgaa caaccgggtg accgtcgccg
    19741 cggactcctc cgtcagcggc tcgacgctcg ccacgatgtg cacgaagcgc ccgtgcacgc
    19801 catcgatccg gggcgcgacg gactgcagcc gcgacaacag ggcgcgcgcc cggaagtcgg
    19861 acagggcgct gccgccctcg agggtcaaca ggtgcaggcc gtgatccgaa ggcgccgaca
    19921 taccccgatt ctctcagcag cgctgggacc gctcgaccag tcatccgctt gctagcgccg
    19981 cagaggtgac gcattccctc
//
LOCUS       DAOPJG010000037        19736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2913_length_19736_cov_7.465678, whole genome shotgun sequence.
ACCESSION   DAOPJG010000037 DAOPJG010000000
VERSION     DAOPJG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 19736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19736
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2913_length_19736_cov_7.465678"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKU75_05325"
     CDS             complement(<1..400)
                     /locus_tag="PKU75_05325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPF80333.1"
                     /translation="MNVLVLLGSLRPESTNRQLANAALAHLPESARAHVSTLPAGLFF
                     YDESLDTDERRPALVDAFRAEVAAADAIIVVTPEYNGSVSGVVKNALDWASRPRGAAA
                     IAGIPALVLAASGSPRGAQWAREAAVRVLQI"
     gene            502..1032
                     /locus_tag="PKU75_05330"
     CDS             502..1032
                     /locus_tag="PKU75_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPF80334.1"
                     /translation="MANPSPDCALPGSCAPARGEERVPLVALLGRLEAAFVAEFDARL
                     ATSPFCALSLAHSRNVLRHLGAGPRRASQIVAMTDVSKQAISQQIAHLERNGYLRTEP
                     DPDDQRARILVLTAKGERAQAHVKATFVEIEQDWGALLPAEDDLTQLRSRLTDLVAAL
                     QGAGPGRPAPPHTEPC"
     gene            1035..1391
                     /locus_tag="PKU75_05335"
     CDS             1035..1391
                     /locus_tag="PKU75_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPF80335.1"
                     /translation="MIAMSREEFELAVGDALDSVPAELMDLLDNVAFFVEDEPPADQP
                     DDLLGIYDGMALTERDSGWGTFNLPDRIVLFRGPLTRMCDSREELLDEIEITVVHEIA
                     HHFGIDDAHLHDLGWG"
     gene            complement(1474..3183)
                     /locus_tag="PKU75_05340"
     CDS             complement(1474..3183)
                     /locus_tag="PKU75_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HPF80336.1"
                     /translation="MSTNAYDRPAEALRSGDKPVTAPPSAGAKKLGGVAGWVDDRLGP
                     AKGVNYLMKKVFPDHWSFMLGEIAMYSMIVCLLTGVFLTFWFIPSAGHTTYDGSYVPL
                     QGVGMSEAYASSLKISFDIKGGLLIRQMHHWAALMFIVALSAHMLRVFFTGAFRKPRE
                     LNWVIGCILSMLALVEGFAGYSLPDDLLSGTGLRAAEGFMQSAPIIGSYLAYMVFDGP
                     FPGEAIIPRLYGVHVLLLPAILVGLFTAHIVMVAVQKHTQYPGPGRTNSNVVGFPVMP
                     VYAAKAGGFFFIVFGFIAVLSALVQINAIWVYGPYDTSAVTAGAQPDWYMGFADGALR
                     LLPGWLEFHAFGYTLSLNVAIGALMLIPIMYGIMAAYPFIEAWVTGDKREHHLLDRPR
                     NQPTRTALGVAALTFYGVLMFAAANDIMAIKLHMSINDITYFFRTAAFVLPVIAFWAT
                     RRVCLSLQRADRNKVLHGRETGRIVRTADGQFFEAHEPLDPYTRWKLVGFEGQQPLEL
                     TSGVDAHGVAAPSNGKWRTRLSRFYFQDVVQPVTPAELEAAHHDGHATEHLEAGETTK
                     ALH"
     gene            complement(3180..4370)
                     /locus_tag="PKU75_05345"
     CDS             complement(3180..4370)
                     /locus_tag="PKU75_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HPF80337.1"
                     /translation="MSSDTDATPRGGSTAYGADDFAAEESSATAVRLDDGHHVVGDGA
                     LPAHFENPGLPPHVERMADTSEKAAKRAERQVAGLFTLSILGTILFLVAYFAFDTAHR
                     SWVPGLGNVSTTNLLFGIGLGLALLGIGLGAVHWAKTLMPDAEVVEERHPMRSSDAAR
                     EEFVGIMRDGGEKAQLFRRPLIKATLGGALGLFSLPLLVQLVGSLGPLPKNHLSLTMW
                     NGEPDGHGGYTPYDKRPRLVTDPEAMPIKAEHVTIGSVYHVLPEGLLTNEELGVEGLE
                     HGQLEEKGKAVVLLLRLDPKDFKPTERGQKALSWGYQGIVAYSKICTHVGCPVGLYER
                     TTHHLLCPCHQSTFDVTDDCHVIFGPAKRPLPQLKIDVDDEGYLIAAEPFHEAVGPSF
                     WERG"
     gene            complement(4374..5159)
                     /locus_tag="PKU75_05350"
     CDS             complement(4374..5159)
                     /locus_tag="PKU75_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPF80338.1"
                     /translation="MNALAARRRHPAAIFLLLLLGLILTGGAYAALAPKPAIAAEASA
                     EQVKEGQELFKTNCATCHGANALGTNVGPNLAGVGAAAVDFQMGTGRMPMAEPGVQAP
                     RGKEVQFSEEQIAAIGAFVASLAPGPAVPAEEYTTGAGGDPGKGGEIFRTNCSMCHNS
                     AGAGGALTRGKYAPKLTGVEGKHIYEALLTGPQSMPVFNDDNISPESKNDLIAYLKAM
                     EEEGNPGGMALGSLGPVPEGLVAWVFGLGVLVGAAYWLGQKGA"
     gene            complement(5188..5766)
                     /locus_tag="PKU75_05355"
     CDS             complement(5188..5766)
                     /locus_tag="PKU75_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HPF80339.1"
                     /translation="MVSVGTIVWLSSELMFFAGLFAMYFTIRQVRPDLWAEHTPTLNV
                     PFAAANTLILVISSVWCQLGVHQAEHGKAFRSGSLLNVGSWGMREWYVLTYIFGAIFI
                     AGQVYEYAHLVHDGITLASDGYGSVFYLTTGFHGMHVTGGLIAFLLIIARTFTTRHYS
                     HAQATGAVVTSYYWHFVDVVWIALFATIYLLK"
     gene            6020..6484
                     /locus_tag="PKU75_05360"
     CDS             6020..6484
                     /locus_tag="PKU75_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80340.1"
                     /translation="MAESSSHAEPATATATGPRPLSVLLYSDDFTTRDAVRAGVGRRP
                     TRDLEVTSWHECATPPAVIEAVANERFDLLILDGEAVPAGGLGLSRQLKNEIYDCPPI
                     LVLTGRPQDVWLAAWSLADAAVPQPLDPIALCAGIEQALASAPAPGPEPAAL"
     gene            6492..7568
                     /gene="trpD"
                     /locus_tag="PKU75_05365"
     CDS             6492..7568
                     /gene="trpD"
                     /locus_tag="PKU75_05365"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPF80341.1"
                     /translation="MSESTGPVSTWPDILTALVTGRDLSYAEATWAMDQVFAGEASPS
                     QVAGFVIGLRAKGETVEELHALADVMLAHANRIPTPEHSIDIVGTGADRAHTVNISTM
                     SALVLAGAGLRVLKHGNRAASSSSGSADVLEALGVDLSLAPERVAAVGDAAGITFCFA
                     QAFHPAMRHAATTRRELGIGTIFNSLGPLTNPGQPTFAAVGAADLRIAELIAGVFAAR
                     KRPALVFRGEDGLDELTISGPSRLWWVRDGQIGEHLIHPDRLGLALAPVETLRGGDPS
                     HNAGVARDVLAGKGRDDGWPVRDAVLLNAGSALALARGDVPAGADTHAVEEALHAGIA
                     AARESIDSGAAAAALDRWVAATRS"
     gene            complement(7704..7985)
                     /locus_tag="PKU75_05370"
     CDS             complement(7704..7985)
                     /locus_tag="PKU75_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPF80342.1"
                     /translation="MITAIVLINAEVDKIPEVAQAIADIDGISEVYSVTGDVDLIAMA
                     RVREHDHLADVIADKLSKVPGILGTQTHIAFRAYSQHDLEAAFSLGIEE"
     gene            complement(8012..9745)
                     /locus_tag="PKU75_05375"
     CDS             complement(8012..9745)
                     /locus_tag="PKU75_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HPF80343.1"
                     /translation="MTITAPARPARAIQGTLDDLGTPLHEVTFVVVDLETTGGSAHAD
                     HITEIGAVKVRGGEVLGEFQTLVNPRVPIPAVISVLTGITNAMVATAPRIEQVLPSFL
                     EFLGDAVVVAHNARFDVTFLKAAASATGNRWPDPVVIDTVLLARALVTRDEAPNHKLA
                     SLARVFHAQVTPDHRALHDAQATVDVLHGLLGRVGGLGVHTLEELATYSVRVPQATRR
                     KRYLADDLPSAPGVYMFKDGQGRVLYVGTSVDIRTRVRSYFTASEQRRRMAEMVRIAE
                     AVTPVVCATALEAQVRELRLIAEHKPRYNRRSRHPEKSAWVKLTVEPFPRLSIVTAVR
                     GDETTYVGPFPSKTAAEDAVAALHDALPLRRCTDRLSPKRPSPACALAEMGRCGAPCT
                     GAQSPQEYAALVARARDALLGDPGTVLSPLEQRMQSLAQDERYEDAASLRDRLLTLLR
                     GMTRSQRLAPLAAAPEIVAARRSPLGGWELVCVRYGRLAGSSHSPRGADPMVYVDALT
                     ASAEVVAPGLAPAPAAYPEETEKILRWLEEPGVRLVSVEGEWTCPVGGAVSAAARFGA
                     TPAASPSPFEA"
     gene            complement(10032..11495)
                     /locus_tag="PKU75_05380"
     CDS             complement(10032..11495)
                     /locus_tag="PKU75_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF80344.1"
                     /translation="MSRDQELARPTKEALPRMTQTTPSWPAHDPATRRWLPESSKPLD
                     PGKVASLLAAEWDRFTADTPESGDHNRRASKSLPLGVTSSFQHWDPYPISIASARGAW
                     LTDVDGRRLLDLSMGFGAMLVGHLNPTIVSYVEEALQSVGTLFVTPSPVSTEVAERFQ
                     RRFGLDMLRFTQSGTESLMYAVRAARAYTERKAIIKIEGGYHGGYDALQVSVKPALED
                     IGPAEAPIPDVPFDVEAGTVHVVPYNNLERLTEILHEHGREIAAVVMEPVIENLAIVV
                     PDEGYLAGVRALCDEHGVLLIFDEVKTGLTAGYAGASQRLGVKPDLVTLAKSIGGGLP
                     LAAFGGTEEVMQVVVDGRMAHFGTYNGNPLVMAAARAVDDICTHEALDAAETINVATL
                     DAVDEIITEFELPAHTVALGVKGCITWSPTPVRNYRDYKATDFGVAELSWLWGVNRGI
                     LTPPGLDEQWLVSLAHKQDQMDVLVSDFRELAQALRS"
     gene            11569..11964
                     /locus_tag="PKU75_05385"
     CDS             11569..11964
                     /locus_tag="PKU75_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF80345.1"
                     /translation="MSRVIHFEIQAENVQRAVAFYQEVFGWQAEDWSEYAGSPYFGVT
                     TGPEGEPGINGAIMSRPGSNPAVGGMVAGAVLTMGTADFDATAAKIEAAGGTVALPKM
                     ALPGMAWQGYFHDTENNVFGIHQPDPDAA"
     gene            complement(12005..14293)
                     /locus_tag="PKU75_05390"
     CDS             complement(12005..14293)
                     /locus_tag="PKU75_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPF80346.1"
                     /translation="MSRTAAESAAARADEQVADPVWHKSACILCECNCGLQVQLDGRR
                     IAKIRGDKEHPGSRGYTCNKALRLDHYQNGGVRLTSPLRRAADGTFEEIDWATAIREI
                     AAGLAAVRDQHGGESVLFYGGGGQGNHLGGAYKGALMAAVGARYSSNALAQEKTGEAW
                     VDAHLAGGHTVGDWERAEVAIFLGKNPWQSHGVPRARTVLQQISKDPARTMIVIDPVR
                     TESADLADIHLQVRPGTDAWCLAAILAILVGEDLLDHDFLAGHTSGAETVVAHVKSID
                     VSAYAAACGIAEEQLRSAARRIAAAGSVSTYEDLGVQQSLNSTLVSYLNKLIWLLTGN
                     FAKPGAMQRHSWVAPLAKWETTYRTTPVTGARMLGGIVPCNAIPDEILTDHPKRFRAM
                     WVDSANPAHSLADTQRFIEAMAALDLVVVLDVALTETARHADYVLPCASQFEKWEATF
                     FTTEHPHNVFQLRAPLLDPLPGTWTEPQIYSAIVRELGATPARAERILRAAAGRGRAA
                     YTLAFAATAVADKRVRGLAPTLLYETFGPTLPDGAASVAILWPLAQRVALTHRAGMKR
                     AGHRSAAALFDAMLTNRSGVVFTEDRPEDSWSYVPHKDKRIPLAIPELLAALDGLASG
                     PHAHTSAQFPVVLMAGQRRAESANTIFRTTAWRARDPEGALRMNPADAQRLGLAAGSR
                     ARVVTARGSAFAVVTVDDRYQPGHAALPNGLGLDLPSGRGEDGPTERTGVALNTLTDG
                     TRRDPFAGTPWHKHVPARIEPA"
     gene            complement(14457..15053)
                     /locus_tag="PKU75_05395"
     CDS             complement(14457..15053)
                     /locus_tag="PKU75_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPF80347.1"
                     /translation="MIDLATAGVDDAVWQLRPDYAACLVTARGVPGGPSDEASDAALT
                     RAERYAAQLLDAQDLAAIAQVAQWREAFRAFGVRPPSATSSLESLLRRAAAGLPRIDR
                     LTDHYNAISIAHLVPFGGEDLDAYAGAPRLVVADGSESFDAVEGGEIVHTTPKPGEVV
                     WRDDRGVTCRRWNWRQCARTRLTPHTTNAVYACAPPRA"
     gene            15247..15552
                     /locus_tag="PKU75_05400"
     CDS             15247..15552
                     /locus_tag="PKU75_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80348.1"
                     /translation="MWIDCNGCPVRGTHCDGCMVTGMLATIPLQPPPEVAQAPGAAPS
                     AAPAEVFVDLDASERAAVTTLVASGLLSAEEAATVRARLDLSAAHLWQSGPPRSATG"
     gene            complement(15458..16873)
                     /locus_tag="PKU75_05405"
     CDS             complement(15458..16873)
                     /locus_tag="PKU75_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80349.1"
                     /translation="MIARLKGAPLAAFVFAATWLAYFGMLQFSVVRLRIKIVVTLTVA
                     AVLLAWWLARRLHLALSPLVVAMLLVGNAVVGLRTRFFSHVPTEWAWTGRGVLAAAAV
                     GAAAALWFRRPGLALGTAFAAYAAVTVIAIRTDPSPRIDVWITLQQAADAMARGENFY
                     AVTWVDSPGIQDAFTYLPWTAVLLAPGRWIAGDVRWMHLVWALVLTVGFWQLGTAARE
                     RRPAAATALDPRWVAAAAGSAILLVPGTLIQVDQAWTEPLLLTGLTWWAVLMTRGRTW
                     WAIVPLALACASKQHLALVLPLLLAWRPFGPARAIATGALTGALIAPWFLTAPADFFR
                     DTVTLLMNFPPIRFANTWYLYFQHYHGITLPFWVTGLIVFGTLALAILLIHRRRPPLG
                     ELLRWSALMLLVANLVNKQAFYNQYWLVGALVALSLIADQADDWAEDRLSRSPNAADP
                     TATGAPPTDRDALAPSRPPRR"
     gene            complement(16870..18657)
                     /locus_tag="PKU75_05410"
     CDS             complement(16870..18657)
                     /locus_tag="PKU75_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HPF80350.1"
                     /translation="MQEFTTPLVTPVVHEHSLADLPDRKAAQSPQHIALRRLAGERWE
                     EVTAAAFAEQVRTVAKGFIAAGLEPGDKVAILSRTSYEWTLLDFAAWAAGLVPVPIYE
                     TSSPDQIHWILSDSHAHAIVMESAKHAQAFDAIRGDLHEIAHVWRLDEGGIRHLTEGG
                     SGVPDAALDDRMANVHRDSTATLIYTSGTTGRPKGCALTHGNFLTLAEQAIAEMPEIL
                     QRPGASTLLFLPLAHVFARFIEVLAIAAGATLAHTADIPNITTHLQAVQPTFVLAVPR
                     VFEKIYNKAEASAVAGGKGAIFTRAAETAIAYSTALQDGRVPLTLRARHALFDRLVYR
                     KLRELMGGHIQYAVSGGAPLGARLGHFFRGVGITILEGYGLTETTAPATVNLPSKVKI
                     GTVGPPLPGVTLRIASDGEIIIRGINVFHDYRNNPEATAEALRDGWFHTGDIGEIDAD
                     GYLKITGRKKEILITAGGKNVVPGLLEDQLRAHPLISQACVVGDQQPFVAALLTLDAE
                     MVPEWTKNAGLGAFGVAEARTNDVVLAELQKAVDRANETVSKAESIRKFAVLPEDFTE
                     EAGQLTPSLKLKRHVVMTQFADDVAALYT"
     gene            18748..19194
                     /locus_tag="PKU75_05415"
     CDS             18748..19194
                     /locus_tag="PKU75_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPF80351.1"
                     /translation="MADSTESSIIIEADPGAVIDVIADLEAYPEWAGEMKATEILAED
                     GDGWPDRVRITLDAGVIKDTYTLDYDWDIAEDGTGSVSWTLVEASVLKAMDGTYTLNA
                     IDKGTRVDYELMVDLKIPVLGMLKRKAEKVIIETALKELKKRVESL"
     gene            19323..>19736
                     /locus_tag="PKU75_05420"
     CDS             19323..>19736
                     /locus_tag="PKU75_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPF80352.1"
                     /translation="MLFTGKGGVGKTTSAAATAVAAARDGIETLVMSTDAAHSLGDAL
                     DRDLGATGTGAAHRIEDHLHALQVRQPALRRFVGRRGRRLRAHRRDAAPAVAARGPAL
                     VHGPRLPDDPQGEPGGVADPRPGHGAPDAERQRVQL"
BASE COUNT         2993 a         6550 c         7239 g         2954 t
ORIGIN      
        1 cgatctgcag gacgcgcacg gcggcctctc gggcccactg cgcgccgcgc ggggagccgg
       61 acgccgccag gactagagcg gggatcccgg cgatcgcagc ggcgccccgg gggcgcgagg
      121 cccaatccag ggcgttcttc acgacaccgg aaaccgagcc gttgtattcc ggcgtgacga
      181 cgatgattgc gtccgccgcg gcgacctcgg cccggaaggc atcgacgagc gcggggcgcc
      241 gctcgtcggt gtccaggctc tcgtcataga agaacaggcc cgccggcagc gtgctgacgt
      301 gggcgcgcgc cgactccggg aggtgcgcga gggccgcgtt cgcgagttgg cggttggtgg
      361 attcgggacg caggctgccg agcaggacca ggacgttcat gatgtctcct gaagcgatgc
      421 ggaaaagtca agtgcttgac caatatgaca ccacgatcca acactgtcaa gtcgcttgac
      481 tattccggta tgctggtggg catggccaac ccctccccgg actgcgcgct tcccggctcg
      541 tgcgcgccgg cgcgcgggga ggagcgggtg ccgctcgtgg cgctgctcgg gcggctggag
      601 gcggctttcg tcgccgagtt cgacgcccgg ctggccacct cgccgttctg cgcgctgtcc
      661 ctggcccact cacgcaatgt gctgcgccac ctgggggcgg ggccgcggcg cgccagccag
      721 atcgtcgcca tgaccgatgt cagcaagcag gccatcagcc agcagatcgc ccatttggag
      781 cgcaatggct acctgcgcac cgagcccgat cccgacgacc aacgcgcccg catcctcgtg
      841 ctgacggcga agggggagcg ggcccaggcg cacgtcaagg cgaccttcgt cgagatcgag
      901 caggactggg gcgcgctcct gcccgccgag gacgacctga cgcagctgcg cagccggctg
      961 accgacctgg tggccgcact ccaaggcgcc gggccggggc gcccggctcc accgcatacc
     1021 gaaccctgct gatcatgatc gcgatgagcc gcgaagagtt cgagctggcc gtcggcgacg
     1081 ccctcgacag cgtgccggcc gaactgatgg acctgctcga caatgtcgcg ttcttcgtcg
     1141 aggacgagcc gccggccgat caaccggacg acctgctcgg catctacgac gggatggcgc
     1201 tgaccgagcg cgacagcggc tggggtacct tcaacctgcc cgatcgcatc gtgctgtttc
     1261 gcggcccgct gacgcgcatg tgcgactcgc gcgaggagtt gctcgacgag atcgagatca
     1321 ccgtcgtgca cgagatcgcc caccacttcg gcatcgacga cgcccacctg cacgacctcg
     1381 gctggggctg agaaacggcc ctggccgcga gcacggggct cgcggccagg gtcagcagtg
     1441 gcgacgccag gagcggcgtc aggaggcgtc ggatcagtgc agcgccttgg tggtctcgcc
     1501 cgcttcgagg tgctccgtgg cgtgcccgtc gtggtgggcg gcctccagtt ccgcgggcgt
     1561 caccggctgg acgacgtcct ggaagtagaa gcggctcagg cgggtgcgcc acttgccgtt
     1621 ggacggtgcg gccaccccgt gggcgtcgac accggacgtg agctccagcg gctgctggcc
     1681 ctcgaagccg acgagcttcc accgggtgta ggggtcgagc ggctcgtggg cctcgaagaa
     1741 ctgcccgtcc gcggtccgca cgatgcgccc ggtctcccgg ccgtgcagca ccttgttgcg
     1801 gtcggcgcgc tgcaggctca ggcagacgcg ccgcgtggcc cagaacgcga tgaccggcag
     1861 gacgaaggcc gccgtccgga agaagtaggt gatgtcgttg atcgacatgt gcagcttgat
     1921 cgccatgatg tcgttggccg cggcgaacat cagcacgccg tagaaggtca acgcggcgac
     1981 accgagggcg gtgcgggtcg gctggttgcg cgggcggtcg agcaggtggt gctcgcgctt
     2041 gtcgccggtg acccaggcct cgatgaacgg gtaggcggcc atgatgccgt acatgatcgg
     2101 gatcagcatc agggcgccga tggcgacgtt gaggctcaag gtgtagccga aggcgtggaa
     2161 ctccagccag cccgggagca gccgcagcgc gccgtcggcg aagcccatgt accagtccgg
     2221 ctgggcgccg gcggtcacgg ccgaggtgtc gtatggcccg tagacccaga tcgcgttgat
     2281 ctggacgagc gcggacagca ccgcgatgaa cccgaagacg atgaagaaga acccgccggc
     2341 cttcgcggca tacaccggca tgaccgggaa cccgaccacg ttggagttgg tccggcctgg
     2401 cccggggtat tgggtgtgct tctgcaccgc caccatgacg atgtgcgcgg tgaacaaccc
     2461 gaccaggatg gccggcagca ggagcacgtg gacgccgtac aggcggggga tgatcgcctc
     2521 accggggaag ggcccgtcga agaccatata ggccaggtag gacccgatga tcggtgcgga
     2581 ctgcatgaag ccttccgccg cccgcagacc ggtgccggag agcaggtcgt cggggaggga
     2641 gtagccggcg aagccctcga ccagggcgag catcgacagg atgcagccga tgacccagtt
     2701 gagttcacgc ggcttgcgga aggcgccggt gaagaagacc cgcagcatgt gcgccgagag
     2761 cgcgacgatg aacatcagcg cggcccagtg gtgcatctgc cggatcagca ggccgccctt
     2821 gatgtcgaag ctgatcttca gggaggaggc gtaggcctcc gacatgccga cgccctgcag
     2881 cggcacatag gacccgtcgt atgtggtgtg gccggcgctg ggaatgaacc agaaggtcag
     2941 gaacacaccg gtcagcaggc agacgatcat cgagtacatc gcgatctcgc cgagcatgaa
     3001 ggaccagtgg tcggggaaga ccttcttcat caggtagttg acgcccttgg ccgggccgag
     3061 gcggtcatcg acccacccgg ccacgcctcc gagcttcttg gcgcccgcgg acgggggcgc
     3121 ggtcacgggc ttgtcgccgc tgcgcagcgc ctcggccggg cggtcgtacg cgttggtgct
     3181 catccacgct cccagaagct cgggccgacc gcctcgtgga acggttcggc agcaatgagg
     3241 tagccctcgt cgtccacgtc gatcttcagc tgcggcaggg gacgcttggc ggggccgaag
     3301 atgacgtggc agtcatcggt cacgtcgaag gtcgactggt ggcacgggca cagcaggtgg
     3361 tgtgtcgtgc gctcgtaaag gccgacgggg cagccgacgt gggtgcagat cttggagtag
     3421 gccacgatgc cctgatagcc ccacgacagc gccttctggc cccgctcggt cggcttgaag
     3481 tccttggggt cgaggcgcag gagcaggacc acggccttgc ccttctcctc gagctggccg
     3541 tgctcgagac cctcgacacc gagttcctcg ttggtgagca ggccctcggg gagcacgtgg
     3601 tagaccgagc cgatcgtgac gtgctcggcc ttgatcggca tcgcctcggg gtcggtgacc
     3661 agccggggac gcttgtcgta tggggtgtag ccgccgtgcc cgtcgggctc gccgttccac
     3721 atggtcagcg agaggtggtt cttcggcagc gggccgaggc tgccgaccag ctgcacgagc
     3781 agcggcaggg agaacagccc gagagcgccg ccgagggtgg ccttgatcag cggccggcgg
     3841 aagagctggg ccttctcgcc cccgtcgcgc atgatgccga cgaactcttc gcgggcggcg
     3901 tcgctcgagc gcatcgggtg gcgctcctcg acgacctcgg cgtcgggcat gagggtcttg
     3961 gcccagtgca cagcgccgag gccgataccg aggagggcca agccgaggcc gattccgaag
     4021 agcaggttgg tcgtggagac gttacccagg cccggcaccc agctgcggtg cgcggtgtcg
     4081 aaggcgaagt aggcgacgag aaacaggatc gtgccgagga tggatagagt gaacaggccg
     4141 gcgacctgcc gctcggcacg cttggcggcc ttctcgctcg tgtccgccat ccgctcgacg
     4201 tgcggtggga ggccggggtt ctcgaaatgg gccggcaacg ctccgtcgcc gacgacgtgg
     4261 tgaccgtcat cgaggcggac ggcggtcgcc gaggactcct cggcggcgaa gtcgtcggca
     4321 ccgtatgccg tggatccacc tcgtggggtg gcgtcggtgt cgctgctcat cggtcaggct
     4381 cccttctgcc cgagccagta cgcagcgccc acgaggaccc cgaggccgaa cacccaggcc
     4441 acgaggccct ccggcacggg accgaggctg ccgagcgcca taccgccggg gttgccttcc
     4501 tcctccatcg ccttcaggta ggcgatcagg tcgttcttgg attcgggcga gatgttgtcg
     4561 tcgttgaaca ccggcatgct ctgcgggccg gtgagcaacg cctcatagat gtgcttgccc
     4621 tcgacaccgg tcagcttggg ggcgtatttg ccgcgggtca gcgccccacc agcaccagcg
     4681 gagttgtggc acatcgagca gttggtgcgg aagatctcgc cacccttgcc cgggtcgccg
     4741 ccagcgccgg tggtgtactc ctcggccggc accgcggggc cgggggcgag ggaggcgacg
     4801 aacgcgccga tggccgcgat ctgctcctcc gagaactgga cttccttccc acgcggggct
     4861 tggacacccg gttcggccat cggcatccgc ccggtgccca tctggaagtc gaccgcggcc
     4921 gcacccacgc cggcgagatt gggtccgacg ttcgtgccca gcgcgttggc gccgtggcag
     4981 gtggcgcaat tggtcttgaa cagctcttgg ccttccttga cctgctcggc cgacgcctcg
     5041 gcggcgatgg cgggcttggg ggcgagggcg gcatacgccc caccggtcag gatcagtccc
     5101 aacaacaaaa gcaggaagat cgcggccggg tgccgtcgcc gtgcggcgag tgcgttcact
     5161 tcggtctctt ttctgttgcg ggggtgatca cttgagcagg tagatcgtcg cgaacagcgc
     5221 gatccacacc acgtcgacga agtgccagta gtacgacgtc acaaccgcgc ccgtggcctg
     5281 ggcgtggctg tagtgcctgg ttgtgaaggt gcgggcgatg atcagcagga acgcgatcag
     5341 gccgccggtg acgtgcatgc cgtggaagcc ggtcgtcagg tagaagacag agccgtagcc
     5401 atccgaggcg agcgtgatgc cgtcgtggac gaggtgggcg tattcgtaga cctggccggc
     5461 gatgaagatc gcgccgaaga tgtaggtcag gacgtaccac tcgcgcatgc cccagctgcc
     5521 gacgttgagc aggctcccgg accggaaggc cttgccgtgc tcggcctggt ggaccccgag
     5581 ctgacaccac accgaggaga tcaccaggat caaggtgttg gcggccgcaa acggcacgtt
     5641 cagcgtcggc gtgtgctcgg cccacagatc ggggcggacc tggcgaatcg tgaagtacat
     5701 cgcgaagaga cccgcgaaga acatcagttc gctggagagc cagacgatgg tccccacgga
     5761 caccatgttg ggacgggtta cggtgcccgt ggcgtgcgcg tagggcgtcg gagacgtgcc
     5821 ggaatgaacg gcggatgctg tggccacggc agcattatgt cggtaaacga atcggcgggg
     5881 gagccgaccc ccccgaaacg ggtgaatcga gtccataacg tgcctcgtcg ggccgcggtt
     5941 ccatcgtggt gcgccccgcc gaaggtccgg cgggcggagg tggcgtgggc cgagcgggtc
     6001 atcccgttag tcttgtccca tggccgagag ctcttcgcac gccgaacccg ccaccgccac
     6061 cgcgaccggg ccccgcccgc tgtcggtgct gctctacagc gacgacttca ccacccgcga
     6121 cgccgtgcgc gccggtgtgg gtcgccggcc gacccgcgac ctcgaagtca cctcgtggca
     6181 tgagtgcgcg acaccgccgg ccgtcatcga ggccgtcgcg aacgaacgct tcgacctgct
     6241 catcctcgat ggcgaggcgg tgccggccgg cgggctgggc ctgtcgaggc agctgaagaa
     6301 cgagatctac gactgcccgc cgatcctcgt gctgaccggc cggccgcagg acgtgtggct
     6361 ggccgcgtgg tcgctcgccg acgccgccgt gccccagcca ctcgacccga tcgccctgtg
     6421 tgccggcatc gagcaggctc tcgcctctgc cccggccccc gggccggagc cggccgccct
     6481 ctgatccccg cgtgagcgag tccacgggcc ccgtctcgac gtggccggac atcctgactg
     6541 ccctcgtgac cggtcgtgac ctgtcgtatg ccgaggccac ctgggcgatg gaccaggtct
     6601 tcgccggcga ggccagtccg tcccaggtgg ccgggttcgt catcggcctg cgagccaagg
     6661 gcgagacggt ggaggaattg cacgccctcg ccgacgtgat gctggcgcac gccaaccgca
     6721 tccccacccc cgaacacagc atcgacatcg tcggcacggg ggccgaccgg gcgcacacgg
     6781 tcaacatctc gacgatgtcg gcgctggtgc tcgccggggc cgggctgcgc gtactcaagc
     6841 acggcaaccg ggccgcctcg tcgtcgtccg gcagcgccga tgttctcgaa gcgttgggtg
     6901 tcgatctgtc gctcgcgccg gagcgggtgg ccgcggtcgg cgacgcggcg ggcatcacct
     6961 tctgcttcgc ccaggccttc catccggcca tgcggcatgc ggcgacgacc cgccgcgaac
     7021 tcgggatcgg cacgatcttc aacagcctcg ggccgctgac caaccccggt cagcccacct
     7081 ttgccgccgt cggcgccgcc gacctgcgga tcgccgaact catcgcgggc gtattcgccg
     7141 cccggaagcg cccggcgctg gtcttccgcg gggaggacgg cctcgacgaa ctgacgatct
     7201 ccggaccctc ccgcctgtgg tgggtgcgcg acgggcagat cggcgagcac ctgatccacc
     7261 ccgaccgcct gggcctggcc ctggcaccgg tcgagacgtt gcgcggcggc gacccgagcc
     7321 acaacgccgg ggtcgcccgg gatgtgctgg cgggcaaggg ccgcgacgac gggtggccgg
     7381 tgcgggatgc cgtcctcctc aacgccgggt cggccctcgc gctcgcccgc ggcgacgtgc
     7441 ctgccggcgc cgacacccac gccgtcgagg aggcgctgca cgccggcatc gccgccgccc
     7501 gggaaagcat cgactccggt gccgccgccg ctgccctcga ccgctgggtc gccgccaccc
     7561 gctcctgacc cctcccgtcg agatgtcatt tcgaccccgt cgagaggtcg attcggcccc
     7621 gtcgaagtga catctcgcgg aggtagggat gccgagtagg tcgcggcgcg gccgctcgac
     7681 ggcggtgcgt gcgagatcgc cggttattcc tcgatgccga ggctgaaggc ggcctccagg
     7741 tcgtgctggg aataggcccg gaaggcgatg tgcgtctgcg tgccgaggat gccgggcacc
     7801 ttgctgagct tgtcggcgat gacgtcggcc aggtggtcgt gttcgcgcac ccgggccatc
     7861 gcgatcaggt cgacatcgcc ggtgacggaa tagacctcgc tgatgccgtc gatgtcggcg
     7921 atcgcctggg cgacctccgg gatcttgtcg acctcggcgt tgatcaggac gatggcggtg
     7981 atcatgccgc caaatctagc tgcctgcggg gtcaggcttc gaaggggctt gggctcgcgg
     8041 cgggggtggc gccgaagcgg gcagcggcgc tgaccgcccc gccgaccggg caggtccact
     8101 cgccctcgac gctgaccagc cgcacacccg gctcttcgag ccaccgcagg atcttctcgg
     8161 tctcttcagg gtatgccgcg ggtgccggcg ccaagccggg cgccaccacc tccgcgctcg
     8221 cggtcagcgc gtcgacatag accatggggt cggcgccgcg ggggctgtga ctgctgccgg
     8281 ccaggcggcc gtagcgcaca cacaccagtt cccacccgcc cagcggggaa cgacgggcgg
     8341 cgacaatctc gggggcggcg gcgagcggcg cgagacgctg gctgcgcgtc ataccgcgga
     8401 gcaacgtgag caggcgatcc cgcagcgatg cggcgtcctc gtagcgctcg tcttgggcga
     8461 gggactgcat gcgctgctcg aggggcgaga gcacggtgcc cgggtccccg aggagcgcgt
     8521 cgcgggcgcg ggcgacgagc gcggcatact cctgcggcga ctgggctccg gtgcacggcg
     8581 cgccgcagcg gcccatctcg gccagcgcgc acgccgggct cgggcgtttg ggggagaggc
     8641 gatcggtgca ccggcgtagt ggcagcgcgt cgtgcagtgc ggcgacggcg tcctcggcgg
     8701 cggttttgga ggggaacggg ccgacatagg tggtctcgtc gccgcggacc gccgtgacga
     8761 tcgagagccg ggggaagggc tcgacggtca gcttgaccca ggcggacttc tcggggtggc
     8821 gggagcggcg gttgtagcgg ggcttgtgtt cggcgatcag gcgcagctcg cggacctggg
     8881 cttcgagggc ggtggcgcac acgacggggg tgaccgcctc ggcgatgcgg accatctcgg
     8941 ccatgcggcg ccgctgctcg gaggcggtga agtaggagcg gactcgcgtg cggatgtcga
     9001 cggaggtgcc gacgtagagg acccggccct gcccgtcctt gaacatgtag acccccggcg
     9061 cgctcggcag gtcgtcggcg agataacgct tgcgccgggt ggcctgcggc acccggaccg
     9121 aataggtggc gagctcttcg agggtatgga cgcccagccc gccgacccgc ccgagcaggc
     9181 cgtggagaac atcgacggtc gcctgggcgt cgtgcagggc gcggtgatcc ggggtgacct
     9241 gggcatggaa gacccgggcc aggctggcga gcttgtggtt gggcgcctcg tcgcgggtga
     9301 ccagggcccg ggcgaggagc acggtgtcga tgacgaccgg gtcgggccag cggttgccgg
     9361 tcgcggaggc ggccgccttg aggaaagtga cgtcgaagcg ggcgttgtgg gccacgacga
     9421 cggcgtcgcc gaggaactcc aggaagcttg gcaggacctg ctcgatgcgc ggggcggtgg
     9481 ccaccatggc gttggtgatg ccggtcagga cggagatgac ggcggggatc gggacgcgcg
     9541 ggttgacgag agtctggaac tccccgagca cttcgccacc gcggaccttg acggccccga
     9601 tctcggtgat gtggtcggcg tgcgcgctgc cgccggtggt ctccaggtcg accacgacga
     9661 aggtgacctc gtgcagcggg gtgccgaggt cgtcgagagt gccctggatc gccctcgcgg
     9721 gtcgggccgg ggcggtgatc gtcatacgca ggacagtaaa gaagcccacc gacaaccccc
     9781 ggcagacacc ccgacggcgg cgtcgcagtg acgagatgtc gggcggttga ccggacacca
     9841 cctcgtgtgc cgcgaactgc ctggcgtagc ccggactccg gcagatatgt gccagggagt
     9901 tcgggctaaa ccaggctgtt cgcgccttcc gaggcgattc ggggttccgg ggcgggtcga
     9961 ggcggtggag ttcgcgctta ccggggttgt tggcggggat tgcgggagtt ggcgggaagt
    10021 gaaggagcag gtcaggagcg cagcgcctgc gccagctcgc ggaagtcgga gacgaggacg
    10081 tccatctggt cttgtttgtg cgcgagcgag acgagccact gctcgtccag cccaggtggg
    10141 gtgaggatgc cgcggttgac accccatagc cacgacagtt cggcgacccc gaagtcggtg
    10201 gccttgtagt cgcggtagtt gcgcaccggg gtgggggacc aggtgatgca ccccttgacc
    10261 ccgagggcca cggtgtgcgc cggtagttcg aactcggtga tgatctcgtc gaccgcgtcg
    10321 agggtggcga cgttgatcgt ctcggccgcg tccagcgcct cgtgggtgca gatgtcgtcg
    10381 acagcgcggg cggcggccat gacgagcggg ttgccgttgt aggtgccgaa gtgcgccatc
    10441 cggccgtcga cgacgacctg catgacctcc tcggtgccgc cgaacgccgc cagcggcagc
    10501 ccgccaccga tcgacttggc cagcgtcacg aggtcgggct tgacgcccag gcgctgcgag
    10561 gccccggcat accctgccgt caggcccgtc ttgacctcgt cgaagatcag caggaccccg
    10621 tgctcgtcgc acagcgcccg cacgcccgcg agatagccct cgtccggcac gacgatggcg
    10681 agattctcga tcaccggctc catgacgacg gccgcgatct cccggccgtg ctcgtgcagg
    10741 atctcggtca gccgctctag gttgttgtag ggcacgacgt ggacggtgcc cgcctcgaca
    10801 tcgaagggca catcggggat cggcgcctcc gctgggccga tgtcctccag ggccggcttg
    10861 accgagacct gcagcgcgtc atacccgccg tggtagccgc cctcgatctt gatgatcgcc
    10921 ttgcgctcgg tgtaagcacg ggcggcgcgg accgcataca tcagcgactc ggtgccggac
    10981 tgggtgaagc gcagcatgtc gaggccgaaa cggcgctgga agcgttcggc gacctcggtc
    11041 gacacgggcg agggggtgac gaacagggtg ccgaccgact gcagcgcctc ttcgacgtag
    11101 ctgacgatcg tcgggttgag gtggccgacg agcatggcac cgaaccccat cgacagatcg
    11161 agcagacggc ggccgtcgac atcggtcagc caggcgccgc gcgccgaagc gatcgagatg
    11221 gggtaggggt cccagtgctg gaacgaggag gtgacgccga ggggcagcga cttgctcgcc
    11281 cgccggttgt ggtcgccgga ctcgggcgta tcggccgtga accggtccca ctccgccgcg
    11341 agcagcgagg cgaccttccc ggggtccaac ggcttcgagc tctcgggcaa ccagcggcgg
    11401 gttgcggggt cgtgcgcggg ccaggacggg gtcgtctggg tcattcgggg gagtgcctcc
    11461 ttggtcggcc gggccagctc ttggtcacgg ctcacctcat catcccaggg cgggcggatt
    11521 cggtgccaca ctgcataccg cagtcgcacg aatcccgagg agttccccat gagccgagtc
    11581 atccacttcg agatccaggc cgagaacgtc caacgcgccg tcgccttcta tcaggaggtc
    11641 ttcggctggc aggccgagga ctggagcgag tatgccgggt ccccctactt cggggtcacg
    11701 accggtccgg agggggagcc ggggatcaat ggcgcgatca tgtcgcggcc agggtccaac
    11761 cccgccgtgg gcggaatggt cgccggggcg gtattgacaa tgggcacagc ggatttcgac
    11821 gccaccgccg ccaagatcga ggcggccggt ggcacggtcg cgctgccgaa gatggcgttg
    11881 cccggcatgg cctggcaggg ctacttccac gacaccgaga acaacgtctt cgggatccac
    11941 cagccggatc ccgacgccgc gtagcgaggc cgcgcgacag ctgctgcccg cgacagctgc
    12001 tggctcaggc gggttcgatc cgggcaggca cgtgtttgtg ccagggcgtc ccggcgaagg
    12061 ggtcgcgccg ggtgccgtcg gtgagggtgt tcagggctac gcccgtccgc tcggtcggcc
    12121 cgtcctcgcc ccggccactg ggcaggtcca gacccagccc gttgggcaat gcggcgtggc
    12181 cgggctggta gcgatcgtcg acagtgacga cggcgaacgc gctgcctcgg gcggtgacca
    12241 cccgcgcccg ggagcccgcg gccagcccga ggcgctgggc gtccgcgggg ttcatccgca
    12301 gcgctccctc ggggtcccgg gcgcgccagg ctgtcgtccg gaagatggtg ttggcgctct
    12361 cggcgcgccg ctggccggcc atgagcacga ccgggaactg cgcgctcgtg tgggcatgcg
    12421 gcccgctcgc caacccatcg agcgctgcca gcagttcggg gatcgcgagc gggatccgct
    12481 tgtccttgtg cggcacatag gaccaggagt cctccgggcg gtcctcggtg aagacgacgc
    12541 ccgaccggtt ggtgagcatg gcgtcgaaaa gcgctgcggc ggaacgatgt ccggcccgct
    12601 tcatcccggc gcggtgggtg agcgcgacgc gctgcgccag cggccacaga atcgccacgc
    12661 tcgcggcgcc gtcgggcaat gtggggccga aggtctcgta gagcagtgtc ggcgccaggc
    12721 cgcgaactct cttgtccgcc accgcggttg ccgcgaaggc gagagtgtat gccgcgcgcc
    12781 cccgccccgc ggcggcgcgc aggatccgct cggcgcgggc cggtgtcgcg ccgagttcgc
    12841 gcacgatcgc cgagtagatc tgcggctcgg tccaggtgcc cggcagcggg tcgagcagcg
    12901 gcgcccgcag ctgaaaaacg ttgtgcgggt gctcggttgt gaagaacgtc gcctcccatt
    12961 tctcgaactg cgaggcgcac ggcagcacat agtcggcgtg cctggcggtc tccgtcaggg
    13021 cgacgtcgag cacgacgacg aggtcgaggg ccgccatggc ctcgatgaac ctctgggtgt
    13081 cggcgaggga gtgggcgggg ttggccgagt cgacccacat ggcgcggaac cgcttcgggt
    13141 gatcggtcag gatctcgtcc gggatggcat tgcacggcac gatgccgccg agcatccggg
    13201 cccctgtcac cggtgtcgtg cgataggtgg tctcccactt cgccagcggc gccacccacg
    13261 aatgacgttg catcgcaccg ggtttcgcga agttgccggt gagcagccag atgagcttgt
    13321 tgagatagga aaccaatgtg gagttgagac tttgctggac gccgagatcc tcgtaggtgg
    13381 acacacttcc tgcggccgcg atccggcggg ccgccgatct gagttgctcc tccgcgatcc
    13441 cgcaggcggc ggcatacgcg ctgacgtcga tcgatttcac gtgcgcgaca acggtttccg
    13501 cgccgctggt gtggccggcg aggaaatcgt gatccaggag atcctccccg accaggatgg
    13561 cgaggatcgc ggcgaggcac caggcgtcgg tgccgggccg cacctgcagg tggatgtcgg
    13621 cgaggtcggc gctctcggtc cgcaccggat cgatgacgat catcgtccgc gccgggtctt
    13681 tcgagatctg ttgcagcaca gtgcgagcgc gcggcacgcc gtgggactgc cacgggttct
    13741 tgcccaggaa gatcgcgacc tcggcccgct cccagtcgcc gaccgtgtgc ccgccggcga
    13801 ggtgggcatc gacccaggcc tcgccggtct tctcctgggc cagggcgttg gaggaatagc
    13861 gtgcgccgac agcggccatg agcgcgccct tgtaggcgcc gccgaggtga ttgccttgtc
    13921 cgccaccgcc atagaagagg acggattccc cgccgtgctg gtcccgcacc gcggccaatc
    13981 cggcggcgat ctcgcggatc gccgtggccc agtcgatctc ctcgaacgtg ccgtcggctg
    14041 cccggcgcag cgggctggtc agccggacgc cgccgttttg atagtggtcg agccgcagcg
    14101 ccttgttgca cgtgtagccg cggctgccgg gatgctcctt gtcgccgcgg atcttcgcga
    14161 tccgccgtcc gtcgagctgc acctgcagtc cgcaattgca ctcgcagaga atgcacgcgc
    14221 tcttgtgcca caccggatcg gcgacctgct catcggctct ggcggcggcg ctctcggccg
    14281 cggtcctgct cacgcgggtc agagtaggcg agtgcgacgg ttcgggtgcc ccctgccggc
    14341 acccgaaccg aaccgtcggg gaccagcttt gcgctcggcg cactgcgcgg cgacccaccc
    14401 cggtcaccgg cgccgggccg ctggtcatga gcacaggagg ccaggcatga ccccggtcat
    14461 gcccgcggcg gggcgcatgc gtagacggcg ttggtggtat gcggtgtcag ccgggtccgc
    14521 gcgcactggc gccagttcca gcggcggcag gtgacgccgc ggtcgtcgcg ccacaccacc
    14581 tcgcccggtt tgggggtggt gtggacgatc tcgccgccct cgacggcgtc gaaggactcg
    14641 ctgccgtcgg cgacgaccag gcgtggggca ccggcatagg cgtccaggtc ctcgccgccg
    14701 aagggcacca ggtgggcgat cgagatcgcg ttgtagtggt cggtgaggcg atcgatccgt
    14761 ggcaggccgg cggcggcccg gcgcagcagc gactcgagac tgctcgtggc cgagggcggt
    14821 cggacaccga aggcgcggaa ggcttctcgc cactgcgcga cctgggcgat cgcggcgaga
    14881 tcctgcgcgt cgagcagctg cgcggcatac cgctcggcgc gggtgagggc cgcatcgctc
    14941 gcctcgtcgc tggggccgcc gggcacgccc cgggcggtga cgagacaggc ggcgtagtcc
    15001 gggcgcagct gccagacggc gtcgtcgacg ccggcggtcg cgaggtcgat catggggggt
    15061 gcgttagccg tcgctcgtgg catcgggagg ttcggtgctt atcggggacc cgccttcccg
    15121 cccgcgagag gcacgtccgg cctggtcggc tcctcggctg agggggttgt cggacctcgt
    15181 ctctagcgtt cttctcagcg ggccgatcgg cggcccggat gacgctcgga cgaaggagaa
    15241 gggcacatgt ggatcgactg caatggctgc ccggtgcgcg gcacccactg cgatgggtgc
    15301 atggtgacgg ggatgctggc gaccatcccg ctgcagccgc cgcccgaggt ggcccaggcg
    15361 ccgggtgcag cgccgtccgc ggcgccggcc gaggtcttcg ttgacctcga cgccagtgag
    15421 cgggcggccg tgacgaccct ggtggcctcg gggctgctca gcgccgagga ggccgcgacg
    15481 gtgcgagcgc gtctcgatct gtcggcggcg cacctgtggc agtcgggtcc gccgcgttcg
    15541 gcgaccggct gagccggtct tcggcccagt cgtcggcctg gtcggcgatc agcgagaggg
    15601 cgaccagggc tccgacgagc cagtactggt tgtagaacgc ctgcttgttg accaggttgg
    15661 ccaccagcag catcagcgcg ctccagcgca gcagttcgcc cagcggcggc cgccggcggt
    15721 ggatcagcag tatggccagc gcgagcgtgc cgaagacgat caggccggtg acccagaagg
    15781 gcagggtgat gccgtggtag tgctggaagt agaggtacca cgtgttcgcg aagcggatgg
    15841 gcgggaagtt catcaacaag gtgacggtgt cgcggaagaa gtccgcaggc gcggtgagga
    15901 accacggggc gatcagggcg ccggtcaggg cgccggtggc gatcgcgcgg gcgggaccga
    15961 agggccgcca ggccagcagc agggggagga ccagcgcgag atgctgcttg ctcgcgcagg
    16021 ccagggcgag ggggacgatc gcccaccacg tccggccgcg ggtcatcagc accgcccacc
    16081 aggtcaggcc ggtcagcagc agcggctcgg tccaggcctg gtcgacctgg atcagcgtac
    16141 ccggcacgag caggatggcc gaccccgcgg cggccgcgac ccagcggggg tcgagcgctg
    16201 tcgcggcggc cggccgccgc tcccgcgcgg ccgtcccgag ctgccagaag ccgacggtca
    16261 gcacgagcgc ccacaccagg tgcatccagc gcacatcgcc ggcgatccag cggcccggcg
    16321 ccagcagcac ggccgtccac ggcagatagg tgaaggcatc ctggatgccg ggggagtcga
    16381 cccaggtgac ggcgtagaag ttctcgccgc gggccatcgc gtcggcggcc tgctgcaagg
    16441 tgatccacac gtcgatccga ggcgatgggt cggtgcggat cgcgatgacg gtgaccgcgg
    16501 catacgccgc gaaggcggtg ccgagcgcga gccccggacg ccggaaccac agcgccgcgg
    16561 ccgccccgac cgccgcggcg gcgagcacgc cccggccggt ccacgcccac tccgtcggca
    16621 catggctgaa gaagcgggtg cgcagcccca cgacggcgtt accgacgagc agcatcgcga
    16681 caacgagcgg gctgagagcg aggtgcagac gccgcgcgag ccaccatgcg agcagcactg
    16741 ccgccacggt gagcgtgacg acgatcttga tgcgcagccg gacgacgctg aactgcagca
    16801 tgccgaaata ggcgagccag gtcgcggcga agacgaacgc cgcgaggggc gcgcccttga
    16861 ggcgggcgat cacgtgtaga gggctgccac gtcgtcggcg aactgggtca tgaccacatg
    16921 ccgcttgagc ttcagcgacg gtgtcagctg tcccgcttcc tcggtgaagt cctcggggag
    16981 cacggcgaac ttgcggatgg actcggcctt ggacaccgtc tcgttcgccc ggtcgacggc
    17041 cttttggagt tcggccagca ccacgtcatt ggtgcgtgcc tcggcgacgc cgaacgcgcc
    17101 gagaccggcg ttcttggtcc actcgggcac catttccgcg tcgagggtca gcagggcggc
    17161 gacgaagggt tgctggtccc cgacgacgca ggcctgggag atcagggggt gggcgcgcag
    17221 ctggtcctcc aggaggccgg ggacgacgtt cttgccgccg gcggtgatca ggatctcctt
    17281 cttgcggccg gtgatcttca ggtagccgtc ggcgtcgatc tcgccgatgt cgccggtgtg
    17341 gaaccagccg tcgcgcagcg cctcggccgt ggcctcgggg ttgttgcggt agtcgtggaa
    17401 gacgttgatg ccccggatga tgatctcccc gtccgaggcg atgcgcagcg tcacgcccgg
    17461 caagggcggc ccgacggtgc cgatcttgac cttggacggc aggttcaccg tcgccggggc
    17521 cgtggtctcc gtcaggccgt agccctcgag gatcgtgatg ccgacgccgc ggaagaagtg
    17581 ccccagccgg gctccgaggg gcgcgccgcc ggagacggcg tactggatgt gcccgcccat
    17641 cagctcgcgc agcttgcgat agacgagccg gtcgaagagc gcgtgccggg cccgcagcgt
    17701 cagcggcacg cggccgtcct gcagcgccgt cgaataggcg atcgcggtct cggcagcccg
    17761 cgtgaagatg gcgcccttgc cgccggccac cgcgctggcc tcggccttgt tgtagatctt
    17821 ctcgaagacc cgcgggaccg ccaggacgaa ggtcggctgg accgcctgca ggtgggtggt
    17881 gatattgggg atgtcggcgg tgtgcgccag cgtcgcgccc gcggcgatcg cgagcacctc
    17941 gatgaaccgc gcgaaaacgt gggccagggg caggaagagc agggtggaag cacccggccg
    18001 ctgcaggatc tccggcatct cggcgatcgc ctgctcggcg agggtgagga agttgccgtg
    18061 cgtgagcgcg caccccttgg gccggccggt ggtccccgag gtgtagatca gggtggcggt
    18121 ggagtcccgg tgcacgttcg ccatccggtc gtcgagggcc gcgtccggca ccccgctacc
    18181 gccctccgtc aggtggcgga taccgccttc gtcgaggcgc cacacgtgcg cgatctcgtg
    18241 caggtcgccc cggatcgcgt cgaaggcctg cgcatgcttg gccgattcca tgacgatcgc
    18301 gtgcgcgtgg gagtcggaga ggatccagtg gatctggtcg ggcgaggagg tttcgtagat
    18361 gggcaccggc accaggccgg cggcccacgc ggcgaagtcc agcagggtcc actcgtatga
    18421 ggtgcggctg aggatggcga ccttgtcgcc gggttccagc ccggccgcga tgaacccctt
    18481 ggccaccgtg cgcacctgct ccgcgaaggc cgcggcggtc acctcctccc aacgctcccc
    18541 ggccaagcgg cgcagcgcga tgtgctgcgg gctttgggcc gccttgcggt ccggcaggtc
    18601 ggcgaggctg tgttcgtgga caactggagt gaccaggggg gtggtgaatt cctgcacgag
    18661 tcaccagcct aattgcgccc ctgctcacca cgagcgtgtt cgtgctcacg cccgacgcgt
    18721 gccaagaccg gcggtacgct tccggccatg gcggacagca ccgaatcgtc gatcatcatc
    18781 gaggccgatc ctggggccgt gatcgacgtc attgccgact tggaggccta ccccgaatgg
    18841 gccggcgaga tgaaggcgac cgagatcctc gccgaggacg gcgacggctg gcccgaccgc
    18901 gtccgcatca ccttggatgc cggagtcatc aaggacacct acaccctcga ctacgactgg
    18961 gacatcgccg aggacggcac cggctcggtc tcgtggaccc tcgtcgaggc aagcgtcctg
    19021 aaggccatgg acggcaccta caccctcaac gcgatcgaca agggcacccg ggtcgattac
    19081 gaactgatgg tggatctgaa gatccccgtc ctcgggatgc tcaaacgcaa ggccgagaag
    19141 gtcatcatcg agaccgccct gaaggaactg aagaagcggg tcgagtccct ctgacccgca
    19201 caccagcgac ggacgacacc gcgccccgca agcggtcacc gcgcaagcag gccggcccca
    19261 cgccggcccc ggaccgggcc cgcgaggcgg ccactccgca aacggctgcc ctgccccgcg
    19321 tcctgctctt cacgggcaag ggcggcgtcg gcaagaccac ctcggccgcg gcgaccgccg
    19381 tcgcggccgc ccgcgacggc atcgaaaccc tcgtcatgtc gaccgatgcc gcccactccc
    19441 tcggcgacgc cctcgaccgc gacctgggcg ccacgggcac cggcgcggcc caccggatcg
    19501 aggaccacct ccacgccctg caggtcaggc aaccggcact tcgacggttc gtgggacgtc
    19561 gtggtcgtcg actgcgcgcc caccgccgag acgctgcgcc tgctgtcgct gcccgaggtc
    19621 ctgcactggt acatggaccg cgtcttcccg acgacccgca aggtgaaccg ggtggtgtcg
    19681 ccgatcctcg gccgggtcac ggggctcccg acgccgaacg acagcgtgtt cagctc
//
LOCUS       DAOPJG010000038        19439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2973_length_19439_cov_7.930407, whole genome shotgun sequence.
ACCESSION   DAOPJG010000038 DAOPJG010000000
VERSION     DAOPJG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 19439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19439
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2973_length_19439_cov_7.930407"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PKU75_05425"
     CDS             <1..726
                     /locus_tag="PKU75_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF80353.1"
                     /translation="TFAQLGLDTDLWTLDLDELRADPDFTGIEAERAEGYGLVGTTAG
                     EGEPALILQGHIDVVPTGDLAKWDGDPFSGAIRDGAVHGRGACDMKAGVAVNIAVARA
                     SHASGLRFERRLVSAIEAFWPIHSALRVLESRRNAHPDPLFAGNPLPYGISVGTITAG
                     DWASSVPDSLVAHGRYNIALNEDPAARPAASSRTLSRRPARRIPGCAIIRPSSPGRVA
                     SSAPAGSPERTRSSPRSPTRRMP"
     gene            723..935
                     /locus_tag="PKU75_05430"
     CDS             723..935
                     /locus_tag="PKU75_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80354.1"
                     /translation="MTGARPLPAAAPYGSNLRLYLGHGGIPTLQYGPGDVRLAHEPRE
                     SVPIQETIDVARSLVAFAATRLGGHR"
     gene            932..1864
                     /locus_tag="PKU75_05435"
     CDS             932..1864
                     /locus_tag="PKU75_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPF80355.1"
                     /translation="MSEPAGPACLDLHCHGALGFEFGGAEAGSRKAAAYHRERGARVV
                     ASLVSAPPPALERQVRTLAPLVADGTVAGIHLEGPCLAPARRGAHDAAALIAPDPRLA
                     DRLAGAAADGGAPGAIVHWTHAPELPGADAFAEALVRLGIRPALGHTAADGATMARAI
                     TTGAEATGAPVLITHLFNAMPPLHHRTGGPVAAALSAAARGDAVLELIADGVHVAPEV
                     IRMVFDLVGPAAIALVSDATPATGLGDGRHRLGELDVDVHDGIARVSDGGAIAGSTAT
                     LPDCMTRAVAMGIDRDAVWHSASRTPATLIVTPM"
     gene            complement(2375..2656)
                     /locus_tag="PKU75_05440"
     CDS             complement(2375..2656)
                     /locus_tag="PKU75_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80356.1"
                     /translation="MPGSSEVIEAAAELLEELDDDEAVALAEPLALAEPPGAVLTTPP
                     GTPAVDEVWLVVPGDDTPVLVVDEVRLRVVLFVVPAGEELVEVLVGGGV"
     gene            2634..2798
                     /locus_tag="PKU75_05445"
     CDS             2634..2798
                     /locus_tag="PKU75_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80357.1"
                     /translation="MTSEDPGIPEKVGSIANDILGGGANWDLIKKTFIGLALAGLAGL
                     AFTVMRPKGR"
     gene            complement(2805..3521)
                     /locus_tag="PKU75_05450"
     CDS             complement(2805..3521)
                     /locus_tag="PKU75_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPF80358.1"
                     /translation="MTPENPSRAPESLLGGGWPADVENPRPIRTFTPRYRVSDLTRER
                     MDTLLPRYAVEQLPVDPQAVFGRVAPLVLEIGSGHGAAAIGYAAAHPGHDVLTAEVHL
                     PGVVRMLAAAQASGVENLRVYPRDAMDLLGGGLAPHTVSAVHLLFPDPWPKVRHAKRR
                     FVQQRTLAAIERILVPGGELLVGTDHPVYAHWTREQLAAFPTARVTQVERPAWKDEDG
                     FEGKGRAAGRPPTYLRVTFG"
     gene            complement(3518..4561)
                     /locus_tag="PKU75_05455"
     CDS             complement(3518..4561)
                     /locus_tag="PKU75_05455"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPF80359.1"
                     /translation="MTAKPTGEALRLALLVALAVAVFAVLARFPEDVPRSAYAPLILL
                     AGLMLSLRHLVVYYGVVLGCLAVGLVLVPTLWNRALIIFLVLVFMMATMLVLARSREG
                     LGVYGTGSEALLRDLRQRHSKLGEIPTLPEGWHAEGVIAGANGDTFLGDVLLSAEGFH
                     PHHAEFCLVDVSGKGLRAGTRAMTLAAALSGLLGQVEPWRFLQAANSYLVRQQWAEGF
                     ATAVHLDLDPVFGHFSVSSAGHPPAMRYSFATGTWEQVTTEAGPALGLMDNMNFPRIS
                     GELHPGDALVLYTDGIIESRENELAGGIDWMGGIAERALRDGSFSGLAKQLTESARGG
                     PDDDRAALVIWRV"
     gene            complement(4633..5106)
                     /locus_tag="PKU75_05460"
     CDS             complement(4633..5106)
                     /locus_tag="PKU75_05460"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HPF80360.1"
                     /translation="MRIHLGGDHAAYELLGELVTFLEEEGHEVVNHGPHTYDAADDYP
                     VFVLRAAQAVATDPGSLGIVLGGSGNGEQMAANRVRGIRAALAYNEELASLAREHNDA
                     QVLSIGARMNTVEQAKAMAKTFVETPFSHDPRHQRRIDMLTAYQEDGTLPPLPTG"
     gene            complement(5106..5747)
                     /locus_tag="PKU75_05465"
     CDS             complement(5106..5747)
                     /locus_tag="PKU75_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HPF80361.1"
                     /translation="MPDDAPEVSRARAQAEMWFDPVCPWAWMTSRWLMEVEQARPVDV
                     TWSVMSLSVLNQGRDLDPDYQALMDAAWAPVRVLTAARAAHGDAVLKPLYDALGERIH
                     HQGRRDYGHIIAEALAEAGLPAELAAAADSESYDEALRASHQRAIDLVGDEVGTPVIS
                     VEGVAFFGPVVSPSPKGEAAGRLWDGCVLVAGTPGFYELKRTRTTGPIFDQEG"
     gene            5896..8448
                     /gene="pepN"
                     /locus_tag="PKU75_05470"
     CDS             5896..8448
                     /gene="pepN"
                     /locus_tag="PKU75_05470"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HPF80362.1"
                     /translation="MPGTNLTREEAQDRAAKIATTRYAVDLDLTTSAETFRSVTTVRF
                     TCSAPGTDTFIDLIAPSVETITLNGESLDPATHFADSRIVLPGLAADNELVVDATCVY
                     MNTGEGLHRFVDPVDDEVYLYTQFEVPDSRRIMAVFEQPDLKAEFSFTVTAPDHWQVV
                     SNAITPEAEPVREGVARWAFPATGRISSYITALVAGPYAVVRDSVTTRAGEIPLGVFC
                     RTSMLEHLDAQNIIDCTKAGFAFFEEMFDTAYPFTKYDQLFTPEYNMGAMENVGCVTI
                     NELYVFRGKVPDAMVERRALTILHELAHMWFGDLVTMKWWNDLWLNESFAEWASTTCQ
                     AEATEWVTAWTTFCTHEKAWAYRQDQLSSTHPIVADIGDLHDVETNFDGITYAKGASV
                     LKQLVAYVGRDQFVAGLRTYFAKHAWGNTTLDDLLDELEVASGRDLRAWSTKWLETAG
                     VNVLRPVLATDDRGLISEAAIEQTVASGDDLLRPHKLKVGSFVLEGDSFRRIASYEVD
                     VDGARTEVPGLIGTAAPDVVLVNDEDLAYAKIRLDERSLAAVLAHPRGFTDTLPHSLV
                     IAALWDMTRDAEMRASDYLPFALAAIEGERDSTLLRTLLAQVSTCLTHYLAPEHRPAA
                     RAEVVARLRALAEGAPGGSDSQLQLLSGYASLLAPGDDPAYLRGLLDGSTTLDGLAVD
                     TDMRWTLVTALAAIGAEGEGDLVAAELDRDDTSNGRERAARARAARPTAEAKAAAWAE
                     GVENASTPNTVIDALAVGFNKADDPALLLPYIAKFLDGVMDVMATRTFAIAEAINGFY
                     PSGLAGPELLEATNAWLDTHPDAPAPARRTIAENRDGVARAVAAQERDRKEA"
     gene            8454..9365
                     /locus_tag="PKU75_05475"
     CDS             8454..9365
                     /locus_tag="PKU75_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPF80363.1"
                     /translation="MPAALPDWTRAEVWDWFDGVPIRVIATIAGAIVVRWLLHRIIRR
                     MVALAIARGEREERSRTGRVLAHATGWSLDRHRQRLATLGTLLRSIITVVVSLVALLT
                     VMSLLGIPLAPLLASAGVGGLALGFGAQSLVKDFLSGVFMIMEDQYGVGDVIDVGEVI
                     GTVEDVTLRITSLRDANGVTWHVRNGEILRVGNKSQGWAQTIVELPIGYDADVEVALT
                     ALDKAVAGFAGDATWRDKVLEPPQVLGVQSLTGGAMTLGILAKCVAGEEFSVQRELRL
                     RAKHALDQAGVPGPRPIGIPPSTGGAP"
     gene            9362..9751
                     /locus_tag="PKU75_05480"
     CDS             9362..9751
                     /locus_tag="PKU75_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HPF80364.1"
                     /translation="MTEQQSVTFYEAVGGHETFRRLVARFYEGVRQDEPLRALYPEED
                     LGPAEERLRMFLEQYWGGPTTYSEQRGHPRLRMRHVPFAVTPDMRDRWLLHMMDAVDS
                     IALQPAHDAILRDYVTRAAYSLVNTMD"
     gene            complement(9755..10543)
                     /locus_tag="PKU75_05485"
     CDS             complement(9755..10543)
                     /locus_tag="PKU75_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPF80365.1"
                     /translation="MRAADHPYFDLTGPVGLAHRGGALYAPNAQRENTIAAFRHAIDL
                     GFRYLETDVHATADGALVAFHDTHLDRVTDGSGAIAALRHTDLAQVRTPTGDGIPLLA
                     EVLEEFPGAHVNIDVKAPGAIAPLVETVERMRVRDRVCVGSFSERRLRAVRKALGPRL
                     ATAAGMTGVASLRFTPWVVSRLLHSPAAVLQIPTHHVVRGRRVELVTPALVRRAHDIG
                     KHVHVWTIDEPDEMHRLLDLGVDGIVTDRPDALREVFAERGLTV"
     gene            complement(10546..12234)
                     /locus_tag="PKU75_05490"
     CDS             complement(10546..12234)
                     /locus_tag="PKU75_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPF80366.1"
                     /translation="MTVTVLDSRPIHARDRADASWWRHAVIYQIYPRSFADGDGNGIG
                     DLPGARSRLPYLKALGVDAIWFSPFYRSPQADAGYDVADYRDVDSLFGTLADFEDLLS
                     DANALGLKVIVDLVPNHTSDEHAWFQAALAAGPGSPERERYLFRDGKGAAGQTPPNNW
                     KSVFGGPAWTRVIEPDGTPGQWYLHLFDVKQPDLNWEHPQVRAEFLDIIDFWLARGVA
                     GFRVDVANALVKDQQLPDWDGFVDDDPRPTSDSPMWDKDGVHEIYRSWRALLNSYDNP
                     ERIMCAESWVNPPERGALYLREDEFHQAFNFDFLECAWRARDLRDVIAKSLHVNDAVD
                     ATTTWVLSNHDVVRHTSRLGLPVGPRRPNGIGADDPQPDAQLGLRRAKAATLLMLGLP
                     GSAYIYQGEELGLPDHTALPGEVRQDPTFMRTEGKEKGRDGCRIPLPWNATQPALGFS
                     ATGRTWLPQPPEYAALAADAQEDVAGSTLEFYRAAIGLRRDRALGLGSLAEVTSYGAD
                     VVAYVNGGAADTLVLVNLGADSVSLPDGAQVLLSSDALSPDGSVPTDTAVWARL"
     gene            complement(12286..13116)
                     /locus_tag="PKU75_05495"
     CDS             complement(12286..13116)
                     /locus_tag="PKU75_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPF80367.1"
                     /translation="MTPAAVLPGTDEWLEGLGAPDDDGDLAAALVRRGAAIGAHLRAG
                     PLERTRKTSAADFVTAADHRAEADIVAALSALRPDDGIVGEEGSTWPSSSGRTWVIDP
                     IDGTFNFANGVGPWCSAIALRHSDGSLVGAIRQEPSDETWVGRVDAHGQGETTLNGRH
                     LPKLAGHDLADGALATYLHARRLADPALLEPWLAAARGAATVRMFGSSSSELAAVATG
                     RTGAFLQCGTADWDWYPGLALVRGVGGVGSVIHHRDRRWHIAGASRVVDQVTHLLTSA
                     "
     gene            complement(13127..13759)
                     /locus_tag="PKU75_05500"
     CDS             complement(13127..13759)
                     /locus_tag="PKU75_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80368.1"
                     /translation="MSGIVLADAESQDDLKRYATIVRTADADAGIRLVASGPVLAAYV
                     CVVPGRGITGGGTVLGLRTFAVEPGEDVDVVVPAAAVLDRIAYGRAHHPDRYEIPVPP
                     MSLFEPWAAQSPPRSGWEPAGELDAEDLIREVRAGITELGEAGTGLAAQVDALRARVW
                     GEPLTGSHAPRGLAFAAYRLRFLTPGEPARLFRSGPWWRLSTAAGHALAR"
     gene            complement(13756..14196)
                     /locus_tag="PKU75_05505"
     CDS             complement(13756..14196)
                     /locus_tag="PKU75_05505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPF80369.1"
                     /translation="MSRSVDVALRWSDMDAYGHVNNVQFLRLLEDARVAAFAEWFDED
                     RSMLDSGVLVARHEIEYLAPLVFRHAPVRIMLWPTAIAGASFDVAYEVRDPDEVGEQL
                     YARAETSLVAYDFARSAPRRLDPREREVLQGLAGEPVAFRWRRR"
     gene            complement(14193..16475)
                     /gene="glgX"
                     /locus_tag="PKU75_05510"
     CDS             complement(14193..16475)
                     /gene="glgX"
                     /locus_tag="PKU75_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HPF80370.1"
                     /translation="MDLWPGKPYPLGATYDGSGVNFAVFSEGATAVELCLIGDDGTEE
                     RVPLQEVDGFVWHSYLPGLQPGQRYGFRVHGPYLPELGLRFDPDKLLLDPYAKAIDGH
                     MEGDPSLFSYPMTDVDRGTKGELNGLDSLGHTLLSVVVNPFFDWDQDRPPKREYHESV
                     IYEAHVKGMTMLHPDIPEALRGTYAGIAHPAMIDHLVSLGVTAIELMPVHQFVDDFHL
                     QDKGLSNYWGYNTIGFFAPHNGYAAYGTRGEQVPEFKSMVKSLHAAGIEVILDVVYNH
                     TAEGNHMGPTLAFRGLDNLAYYRLVQSNPAHYYDTTGTGNSLLMRNPHVLQLIMDSLR
                     YWVTEMHVDGFRFDLAATLARQFHEVDKLSAFFDLIQQDPVVSQVKLIAEPWDIGEGG
                     YQVGNFPPLWTEWNGKYRDTVRDFWRGEPATIGEFASRITGSSDLYEHSGRKPIASIN
                     FVIAHDGFTLRDLVSYNDKHNAANGEDNRDGESHNRSWNCGVEGPTDDPVIRALRIRQ
                     QKNFITTLLLSQGVPMLAHGDEVSRTQGGNNNAYCQDNEIAWMNWDLDADQRDLLDFT
                     RRVLQLRKENPVFRRRRFFAGSADHGGESEVHDISWFTPAGSQMSESDWRNGHARSIM
                     VFLNGQAIPEPDVRGMRIKGDSFLLIFNAHHEMLPFTLPIADYGLAWKIEIDTGATET
                     DDSIHTAGSVVEVAPRSMLVLRHVSEKEAAPPRGAAKAPETRGVAAADPDATTQAAQP
                     ESAGAPRTGPKRSRGKGKRS"
     gene            16558..17316
                     /gene="fabG"
                     /locus_tag="PKU75_05515"
     CDS             16558..17316
                     /gene="fabG"
                     /locus_tag="PKU75_05515"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPF80371.1"
                     /translation="MSESTRVAVVTGAARGIGAATAMRLAKDGNAVAVLDLDEGACAG
                     TVDAITAAGGRALAVGVDVSDEAQVQAAVTRIAEELGAPVILVNNAGIIRDNLIFKMT
                     TADWDAVMGVHLRGAFLMVRECQKYMTQEKYGRIVNLSSSSAQGNRGQANYSAAKAGL
                     QGFTKTLAIELGRFGVTANAVAPGFIQTDMTAATAERIGMPFDEFIAATAKTIPVARV
                     GQPDDIAATISFLCSEEAGFVSGQVIYVAGGPLD"
     gene            complement(17514..18839)
                     /locus_tag="PKU75_05520"
     CDS             complement(17514..18839)
                     /locus_tag="PKU75_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPF80372.1"
                     /translation="MSDVSRRAALAAGLAAGVTTPLSTTAEAAAWPGPDATADDYPQF
                     MADSGHSGWARTETTVTVATVGSLGQLWQRPVAESGWAPATGDPTVVGNGVFLGGRTV
                     VRYDAQTGTRVWSRSIGAVVPTTPVYAHGLVVATGLDGSDSHLGAIDARTGALVWKRR
                     LARSGFASPSIAGSTVLVGSEDRVQAFDLRTGGRRWGWVDPEYWAGMFGSPSAAGGLV
                     IAGNEGNSRVTALNLSDGKVAWQNRLPGTNGWTMDGFGIAVDRASGRAFVGSTGGSYS
                     GAVRAVSVADGTTLWQTELGKSVWRAVAWSGSTVLANAETDLVALDAATGVIRWRFTN
                     EAWLSPGMVCDGGVVLIGVPAGAVLDTPALLGFSIGSGRRLLTLALPGGAGYEPMGAA
                     ISRGRVFLPQWNRLLCLGPGGGPVGRDRSTPPPRRRSPVAPVHRQETRR"
     gene            complement(18929..>19439)
                     /locus_tag="PKU75_05525"
     CDS             complement(18929..>19439)
                     /locus_tag="PKU75_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF80373.1"
                     /translation="LDLMVNNTGINPAYGPILDTPLDAARKILDTNVVAALSWTQKVV
                     AAGLADGGRGAIVNVASVAGIGASGVIGMYGVSKAALIHLTVELGYQLGPAVRVNAVA
                     PAVVKTKFAEALYDGREEQVAAPYPLNRLGVPEDIAGAVSFLLSSDAGWITGQTLVVD
                     GGVTLVAGL"
BASE COUNT         3024 a         6907 c         6621 g         2887 t
ORIGIN      
        1 accttcgccc agctcggctt ggacactgac ctgtggacgc tcgatctcga cgagttgcgg
       61 gccgaccccg acttcacggg catcgaggcc gagcgcgccg agggttacgg gctggtcggc
      121 accaccgcag gtgagggaga gccggcgctc atcctgcaag gccatatcga cgtcgtcccc
      181 acaggcgatt tggcgaagtg ggacggcgat cccttctccg gcgcgatccg cgacggtgcg
      241 gtgcacggcc gcggggcctg cgacatgaaa gccggcgtgg cggtcaatat cgccgtcgcc
      301 cgggcgtcgc acgcgagcgg gctgcgcttc gagcgccggc tggtctcggc gatcgaggcg
      361 ttctggccca tccacagcgc gctacgcgtg ctggagagcc ggcgcaatgc ccaccccgat
      421 ccgctcttcg cgggcaatcc cctgccctac ggcatcagcg tcggcaccat caccgccggc
      481 gactgggcga gcagcgtgcc cgactcgctg gtcgcgcacg ggcgctacaa catcgccctc
      541 aacgaggatc cggccgcgcg gcccgccgcg agttcgagga cgctgtcgcg tcggcctgcg
      601 cgacggatcc ctggctgcgc gatcatccgg ccatcgtcac ctggccgggt ggccagttcg
      661 gctccggccg gctcgccgga acgcacccgc tcatcaccga ggtcgccgac gcggcgtatg
      721 ccgtgaccgg cgcccgcccg ctgcccgccg ccgcccccta cggctcgaac ctgcggctct
      781 atctggggca cggcggcata ccgacattgc agtacggacc gggggacgtg cgactcgccc
      841 acgagccgcg cgagtcggtt cccatccagg agaccatcga cgtcgcccgg tcactcgtcg
      901 cgttcgccgc cacccgcctc ggcggacacc ggtgagcgaa cccgccggcc cggcgtgcct
      961 cgacctgcat tgccacggcg cgctcggctt cgagttcggc ggcgccgagg cgggatcacg
     1021 gaaggcggcg gcataccacc gggaacgcgg agcgcgtgtc gtcgccagcc tcgtcagcgc
     1081 accgccgccg gcgctcgaac gacaggtgcg caccctcgcc ccgctcgtcg ccgacggcac
     1141 ggtcgccggg atccatctcg agggtccctg cctcgccccg gcgcgccgcg gcgcccacga
     1201 cgccgccgca ctgatcgccc ccgacccacg cctcgccgac cgcctggccg gggccgccgc
     1261 cgacggcggg gcgcccggcg cgatcgtgca ctggacccac gcgccggagt tgcccggcgc
     1321 ggacgccttc gcagaagcat tggtgcgctt gggaatccgg ccggcgctcg ggcatacggc
     1381 tgccgatggg gccaccatgg cgcgcgccat cacaaccggc gccgaagcga cgggagcccc
     1441 tgtcctgatc acccacctgt tcaacgctat gccgcccctg catcaccgga cgggcggccc
     1501 ggtcgcggcc gcgctgtcgg cggcggctcg tggcgatgcg gtgctcgaac tcatcgccga
     1561 cggggtccat gtggcgcccg aggtcatccg gatggtcttc gacctggtcg gccccgcagc
     1621 gatcgcgctg gtctccgacg cgacgcccgc caccgggctc ggcgacgggc ggcaccggct
     1681 cggtgagctc gacgtcgacg tgcacgacgg gatcgctcgc gtgtccgacg gaggcgccat
     1741 agccgggagc actgccacct tgccggactg catgacccgc gcggtggcga tggggatcga
     1801 ccgggacgcc gtgtggcaca gcgcgagccg caccccggcg accctcatcg tgacacccat
     1861 gtgacgtctg ggaatgatgt gaaccgggag gcggactgat agaatccgac ccgggtgcgc
     1921 aggctcgatc tgtctgctgc ccgctccggg ttgcgtcgcg ggacgcaccg gctttcacct
     1981 cggaggaacc catgaccgat cgcaagtcca cctcgcgacg cgggccgcgc accacccgcc
     2041 gggcgatcgc ccttccggcc gtcgtcatgg cggtcgtctg tggtgcggcc gtcgcgccgg
     2101 ccaccgccgg ggtcgtggcc gaggggttgg gcaccgtcat ctccagggca acgccgaccg
     2161 acaccacgtc gtccgagtcg agcaccgggt tcaccagccc cgattcgacc tccgacacgc
     2221 cgacgaccga tcccacgacg gacccgacaa cggactcgcc gaccttcacg ccgccgccgg
     2281 acccggagac gacgaccacg acgtcagtgt cctcgacgtt cacgccgccg ccggacccgg
     2341 agacgaccac gtcgacgacg gatccgacca cgtcctacac cccgccgccc accagcacct
     2401 ccaccaactc ctcaccggcc ggcacgacga acagcacgac ccgcagtctt acctcgtcca
     2461 cgacgagcac cggggtgtca tcgccaggca cgacgagcca gacctcgtcc acggccgggg
     2521 tccccggagg cgtcgtcagc accgcccccg gcggctcggc cagcgcgagc ggctcggcca
     2581 gcgcgacggc ctcgtcgtcg tccagttcct ccagcagctc cgccgccgcc tcgatgacct
     2641 cggaggaccc gggcatcccg gagaaggtcg gcagcatcgc caacgacatc ctcggcggcg
     2701 gcgccaactg ggacctgatc aagaagacct tcatcggtct ggctctggcg ggtctcgccg
     2761 gcctggcctt caccgtgatg cgcccgaaag gccgctgatc tccgtcagcc gaaggtgacg
     2821 cgcagatagg tcgggggccg gcccgcagcg cgccccttgc cctcgaaccc gtcctcgtcc
     2881 ttccacgccg gccgctcgac ctgcgtcacg cgcgccgtcg ggaaggccgc gagttgctcg
     2941 cgggtccagt gggcatagac cgggtggtcg gtgccgacga gcagttcgcc gccgggcacg
     3001 aggatgcgtt cgatcgccgc cagggtgcgt tgctggacga agcggcgctt cgcgtgtctg
     3061 accttgggcc acgggtcggg gaagagcaag tggaccgcgc tgaccgtatg cggcgccagt
     3121 cccccgccga gcaggtccat cgcatcccgc ggatagaccc gcaggttctc caccccgctc
     3181 gcctgcgctg ccgcgagcat ccggaccact ccgggcaggt gcacttccgc ggtcaggacg
     3241 tcgtggccag ggtgggcggc ggcatacccg atcgctgcgg cgccgtgacc ggacccgatc
     3301 tcgaggacca gcggcgcaac ccggccgaag accgcctgcg gatccacggg gagctgctcg
     3361 acggcatacc tcggaaggag cgtgtccatc cgctcccggg tgagatcgga gacgcggtag
     3421 cgcggggtga aggtgcggat ggggcgcggg ttctcgacat cggcgggcca gccgccgccg
     3481 agcagcgatt ccggtgcccg gcttgggttt tcgggcgtca tacgcgccag atcaccaagg
     3541 cggcgcggtc gtcgtcgggg ccgccgcgag ccgactcggt cagttgcttc gcgaggccgg
     3601 agaagctgcc gtcgcgcagc gcgcgctcgg cgatgccgcc catccagtcg atgccgccgg
     3661 ccagttcgtt ctcgcgcgac tcgatgatcc cgtcggtgta gagcacgagc gcatcgccgg
     3721 gatgcagctc gccgctgatc cgggggaagt tcatattgtc catcagcccg agcgccgggc
     3781 cggcctcggt cgtgacctgc tcccaggtgc cggtggcaaa ggagtaccgc atcgccgggg
     3841 ggtggccggc gctgctgacc gagaagtgcc cgaacaccgg atcgaggtcg aggtgcacgg
     3901 cagtggcgaa gccctcggcc cactgctgcc gcacgagata actgttggcg gcctggagga
     3961 aacgccacgg ttcgacctgc ccgagcagcc cgctcagcgc cgcggccagg gtcatcgccc
     4021 gcgtgccggc gcgcagtcct ttgccgctga cgtcgacgag gcagaactcg gcgtggtgcg
     4081 ggtggaagcc ctcggccgac agcagcacgt cgccgaggaa ggtgtcgcca ttggcgccgg
     4141 cgatgacccc ttcggcatgc caaccctcgg gcagcgtcgg gatctccccg agcttcgaat
     4201 ggcgttggcg cagatcacga agcagcgcct cgctgccggt gccgtagacc cccagacctt
     4261 cgcgggatcg ggccagcacc agcatcgtcg ccatcatgaa caccaggacc aggaagatga
     4321 tcagcgcccg gttccacagg gtcgggacga gcacgaggcc gacggccagg caaccgagaa
     4381 cgacgccgta gtagacgacg aggtgacgga gggacagcat gagccccgcg agcaggatga
     4441 gcggggcgta tgccgagcgc ggcacgtcct cggggaaccg cgccaggacc gcgaagaccg
     4501 cgaccgcgag cgcgaccagc aacgccaatc gcagcgcttc gcctgtcggc ttggccgtca
     4561 agcgggtgcg aatcctgttg cgtgagcccg cgcgggaggc aacccgcgca gccgagagcg
     4621 acgtcgccat cgtcagccgg tgggcaaggg cggcagggtg ccgtcctcct ggtaggcggt
     4681 gagcatgtcg atgcggcgtt ggtggcgcgg gtcgtgggag aagggtgttt cgacgaacgt
     4741 cttggccatc gccttcgcct gctcgacggt gttcatgcgg gcgccgatcg agagcacctg
     4801 ggcgtcattg tgctcgcggg ccagtgacgc cagctcctcg ttgtaggcca gcgccgcccg
     4861 gatgcccctc acccggttgg ccgccatctg ttcgccattg ccggagccgc cgagcacgat
     4921 gccgagggat ccgggatcgg tcgcgaccgc ttgcgccgcc cgcagcacga agacgggata
     4981 gtcgtcggcg gcgtcgtagg tgtgcggtcc gtggttgacg acctcgtgac cctcctcctc
     5041 gaggaaggtc accagttcgc cgagcagttc gtaagccgcg tggtcgcctc cgagatgaat
     5101 gcgcatcagc cctcctggtc gaagatcggc cccgtggtgc gggtgcgctt gagttcgtag
     5161 aagccgggag ttccggcgac caggacgcac ccgtcccata gtctgccagc cgcctccccc
     5221 ttgggagaag ggctgacgac cggcccgaag aaggcgacgc cctcgacgct gatgaccggg
     5281 gtgccgacct cgtcgccgac cagatcgatc gcgcgctggt gggaggcccg caacgcctcg
     5341 tcatacgact cggagtcggc ggcagcggca agctccgccg gcaagccggc ctcggccagc
     5401 gcttcggcga tgatgtgccc gtagtccctg cggccctggt ggtgaatgcg ctcgcccagc
     5461 gcgtcataga gcggcttgag caccgcgtcg ccatgcgccg cacgggcggc tgtcaacacc
     5521 cggacgggcg cccacgcggc gtccatgagg gcctgatagt cgggatcgag atcgcgaccc
     5581 tggttgagga ccgacaacga catgaccgac cacgtgacat cgaccgggcg ggcctgctcg
     5641 acctccataa gccagcgaga ggtcatccac gcccacggac atacggggtc gaaccacatc
     5701 tcggcctgcg cgcgggcacg gctgacctcg ggagcatcgt caggcatgaa acaaatgtat
     5761 cgtgcaaatc ctgatcgccc tccacccttg cgcgtgggag ggcgccgtcc gagagccagg
     5821 gaaagttcgg cggcgccgtg acaggatgac tccttgtggc cccgatcggg ccgtcgtcga
     5881 caacaggagc taccggtgcc gggaacgaat ctgacccgcg aagaggcgca ggaccgcgcc
     5941 gccaagatcg ccacgacgag atatgccgtc gacctcgacc tgaccacgtc cgcggagacc
     6001 ttccgcagcg tgacgacggt gcggttcacc tgctcggccc ccggcacgga caccttcatc
     6061 gacctgattg cccccagcgt cgagacgatc acgctcaacg gcgaatctct ggaccccgca
     6121 acgcatttcg cagattcgcg gatcgtcctg cccgggctcg ccgccgacaa cgaactcgtc
     6181 gtcgatgcga cctgcgtcta tatgaacacc ggtgagggat tgcaccgttt cgtcgatccg
     6241 gtcgacgacg aggtctacct ctacacccag ttcgaggtgc ccgacagccg ccgcatcatg
     6301 gccgtcttcg agcagcccga tctgaaagcc gagttcagct tcaccgtcac cgcaccggac
     6361 cactggcagg tcgtgtcgaa cgcgatcacg cccgaagcgg agccggtgcg cgaaggcgtg
     6421 gcccggtggg ccttccccgc gaccggacgg atctcctcct atatcaccgc cctcgtcgcc
     6481 ggaccgtatg ccgtggtgcg tgacagcgtg accacccgcg ccggcgagat ccccctcggc
     6541 gtcttctgcc gcacctcgat gctcgagcac ctcgatgccc agaacatcat cgactgcacc
     6601 aaggccggtt tcgccttctt cgaggagatg ttcgacaccg cctacccctt caccaagtac
     6661 gaccagctct tcacgccgga atacaacatg ggcgcgatgg agaacgttgg ctgcgtgacc
     6721 atcaacgagc tctacgtctt ccgcggcaag gtccccgacg ccatggtcga gcgtcgagcg
     6781 ctgacgatcc tgcacgaact cgcccacatg tggttcggcg acctcgtcac catgaagtgg
     6841 tggaacgacc tgtggctcaa cgaatccttc gccgagtggg cgtccacgac ctgccaggcc
     6901 gaggccaccg agtgggtgac cgcctggacg accttctgca cccacgagaa ggcctgggcc
     6961 taccgccagg accagctctc ctcgacccac ccgatcgtcg cggacatcgg tgacctgcac
     7021 gacgtcgaga ccaacttcga cggcatcacc tacgccaagg gcgcctcggt cctgaaacaa
     7081 ctcgtggcgt atgtcggccg ggaccagttc gtcgccggcc tgcgcaccta cttcgccaag
     7141 cacgcgtggg gcaacacgac gctcgatgac ctgctcgacg aactcgaggt cgcgtccggc
     7201 cgggacctgc gcgcctggtc caccaagtgg ctcgagacgg ccggggtcaa tgtgctgcgg
     7261 ccggtgctgg ccaccgacga ccgtgggctg atcagcgagg cggccatcga gcagaccgtc
     7321 gcttctgggg acgacctcct ccggccgcac aagttgaaag tcggatcctt tgtgctggaa
     7381 ggcgattcgt tccggcgtat cgcttcctac gaggtcgacg tcgacggcgc ccgcaccgag
     7441 gttcccggcc tgatcgggac ggcggcaccg gacgtggtgc tcgtcaacga cgaggacctc
     7501 gcctacgcca agatccgtct cgacgagcgc tccctggccg cggtcctggc tcacccgcgc
     7561 ggcttcaccg acaccctccc ccactcgctg gtcatcgcgg ccctgtggga tatgacccgg
     7621 gacgccgaga tgcgcgcgag tgattatctg cccttcgcgc tcgccgccat cgagggcgaa
     7681 cgcgactcga ccctgctgcg cacgctgctc gctcaggtgt ccacctgtct gacccactac
     7741 ctcgcgcccg agcaccggcc ggcggcccgg gccgaggtcg tcgcccggct gcgggcgctc
     7801 gccgagggtg cgcccggcgg cagcgactct cagctccagt tgctcagcgg gtatgcctcc
     7861 ttgcttgccc cgggcgacga cccggcatac cttcgcgggc tgctggacgg ctcgacgacc
     7921 ctcgacggcc tcgccgtcga caccgacatg cggtggacgc tggtgaccgc gctggccgcc
     7981 atcggcgccg aaggcgaggg cgatctcgtt gcggcggagc tggatcgcga cgacacctcc
     8041 aacggacggg agcgtgccgc ccgggcccgc gccgcccggc cgacggcgga ggccaaggcg
     8101 gcggcgtggg ccgagggggt cgagaacgcc agcaccccca acacggtcat cgacgccctc
     8161 gcggtgggct tcaacaaggc cgacgacccg gcgctgctgc tgccctatat cgcgaagttc
     8221 ctcgacggcg tcatggacgt catggcgacc cgcaccttcg ccatcgccga ggccatcaac
     8281 ggcttctatc cgagcggact ggccggtccc gaactgctcg aggcgaccaa cgcctggctc
     8341 gacacccatc ctgacgcgcc ggcgccggcc cggcgcacca tcgccgagaa ccgcgacggc
     8401 gtcgcgcgcg ccgtcgccgc gcaggaacgc gaccgcaagg aggcctgatc ccggtgcctg
     8461 ccgccctccc ggactggact cgggccgagg tctgggactg gttcgacggt gtgcccatcc
     8521 gggtcatcgc caccatcgcc ggcgccatcg tcgtgcgctg gctgctgcac cggatcatcc
     8581 ggcgcatggt ggccctggcg atcgcgcgcg gcgaacggga ggaacgttcg cgcaccggac
     8641 gcgtgctcgc ccacgccacc ggctggtcgc tggaccggca ccgccagcgc ttggcgacgc
     8701 tcggcaccct gttgcgcagc atcatcaccg tcgtcgtctc cctcgtggcg ctgttgaccg
     8761 tgatgtcgct gctcggcata ccgctggcgc cgctgctcgc ctccgccggc gtcggcggcc
     8821 tcgcgctcgg cttcggcgcg cagagcctcg tcaaggactt cctgtccggg gtgttcatga
     8881 tcatggagga ccagtacggc gtcggcgacg tcatcgacgt cggggaggtc atcggaaccg
     8941 tcgaggatgt caccctgcgc atcacgtccc tgcgggacgc caacggcgtg acctggcacg
     9001 tgcgcaatgg cgagatcctg cgcgtgggca acaagagcca gggctgggcg cagacgatcg
     9061 tcgaactgcc gatcgggtat gacgcggacg tcgaggtcgc actcacagcg ctcgacaagg
     9121 ccgtcgccgg gttcgcgggc gatgccacgt ggcgggacaa agtgctcgag ccgcctcagg
     9181 tcctcggcgt ccagtccctc accggcggcg cgatgactct ggggattctc gccaaatgcg
     9241 ttgcgggaga ggaattctcc gtccagcgcg agctgcgttt gcgcgcgaag cacgccctcg
     9301 accaggcggg cgtacccggt ccccggccga tcggcatacc acccagcact ggaggtgccc
     9361 catgaccgag caacaatccg tcaccttcta cgaggccgtc ggcggacacg agacctttcg
     9421 ccggctcgtc gcgcgcttct acgagggcgt gcgccaggac gagccgctgc gcgcgctcta
     9481 tccggaggag gatctcgggc cggccgagga acgactgcgg atgttcctcg agcagtactg
     9541 gggcggcccg acgacctact ccgagcagcg tggccatccg cggctgcgga tgcggcacgt
     9601 gcccttcgcg gtcactcccg acatgcgcga ccgctggctg ctgcacatga tggacgccgt
     9661 ggattccatt gccctgcaac ccgcccacga tgcgatcctg cgcgactacg ttacgcgcgc
     9721 ggcctacagc ctcgtcaaca cgatggatta gccctcacac cgtcaggccg cgctccgcga
     9781 agacctcgcg aagggcgtcg ggccgatcgg tgacgatgcc atcgacgccg agatcgagga
     9841 ggcggtgcat ctcgtccggc tcgtcgatgg tccacacgtg gacatgcttg ccgatgtcgt
     9901 gggcccgccg caccagcgcc ggcgtgacca actcgacccg tcggccccgg acgacgtgat
     9961 gggtggggat ctgcagcact gcggccggcg aatgcagcag ccggctcacg acccacgggg
    10021 tgaaacgcag cgaggcgaca ccggtcatgc cggcggccgt cgcgagccgc gggccgagcg
    10081 ccttgcgcac ggcacgcagc cggcgttcgg agaaggagcc gacacacacc cggtcgcgca
    10141 ctcgcatgcg ctccaccgtc tcgacgagcg gcgcgatcgc gccgggcgcc ttgacgtcga
    10201 tattgacgtg ggcgccgggg aactcctcga ggacctccgc gagcagcggt atgccgtcgc
    10261 ccgtcggtgt ccgcacttgc gcgaggtcgg tgtggcgcag cgccgcgatc gccccggacc
    10321 cgtcggtcac ccggtccagg tgcgtgtcgt ggaaggcgac gagcgcgccg tcagcggtgg
    10381 cgtggacgtc ggtctcgaga tagcgaaatc ccaggtcgat cgcgtgccgg aacgcggcga
    10441 tcgtgttctc ccgctgggcg ttcggcgcgt agagcgcgcc cccgcggtgg gccagcccca
    10501 ccgggccggt caggtcgaag taggggtggt cggccgcacg catcgtcaga gtctcgccca
    10561 cacggccgtg tcggtcggca ccgaaccatc ggggctgagc gcgtccgagg acaacagcac
    10621 ctgagcgccg tccggcagcg acacggagtc cgcgccgagg ttgacgagca ccagtgtgtc
    10681 cgccgcgccg ccgttgacat aggcgacgac gtccgcgccg tagcttgtga cctccgccag
    10741 gctgccgagg ccgagagccc ggtcccggcg cagaccgatg gccgcccggt agaactccag
    10801 cgtggatccg gcgacgtcct cctgcgcatc ggccgccagg gcggcatact ccggcggctg
    10861 gggcagccac gtccggccgg ttgcgctgaa gccgagggcg ggctgagtgg cgttccaggg
    10921 caacgggatc cggcagccgt cccggccctt ctccttgcct tcggtgcgca tgaaggtcgg
    10981 gtcctggcga acttcaccgg gcagcgccgt gtggtccggg agcccgagtt cctcgccctg
    11041 gtagatgtag gccgaccccg gcagacccag catcagcaag gtcgcggcct tggcgcggcg
    11101 taggcccagc tgggcgtccg gttgcgggtc gtcggcgccg atgccgttgg ggcgccgagg
    11161 gccgaccggc aggccgagcc gggaggtatg ccgcacgacg tcgtgattgc tcaacaccca
    11221 ggtcgtcgtc gcatcgaccg catcgttgac gtgcaacgac ttggcgatga cgtcgcgcaa
    11281 atcccgtgcc cgccaggcgc attcgaggaa gtcgaagttg aaggcctggt ggaactcgtc
    11341 ctcgcgcaga tagagcgcgc cgcgctccgg cggattgacc caggactcgg cgcacatgat
    11401 gcgctcggga ttgtcgtagg aattcagcag ggcgcgccac gagcgataga tctcgtgcac
    11461 accgtccttg tcccacatgg gcgaatccga agtcggacgc gggtcgtcgt cgacgaaacc
    11521 gtcccagtcg ggcagctgct ggtccttcac gagggcgttg gcgacgtcga cccggaatcc
    11581 cgcgacgccg cgggccaacc agaagtcgat gatgtcgagg aactcggcgc gcacctgcgg
    11641 gtgctcccag ttcagatccg gctgcttgac gtcgaagagg tgcagatacc actgccccgg
    11701 ggtcccgtcc ggctcgatca cgcgggtcca ggccgggccg ccgaagaccg acttccagtt
    11761 gttcggcggg gtctgccccg cggcgccctt cccgtcccgg aagaggtatc gctcgcgctc
    11821 cgggctgccc ggaccggcgg ccaacgccgc ctggaaccac gcgtgctcat cggaggtgtg
    11881 attgggcacc aggtcgacaa tcaccttcag ccccaacgca tttgcgtcgc taagcagatc
    11941 ctcgaagtcg gcgagcgtgc cgaacagcga gtcgacgtcg cggtagtcgg cgacgtcata
    12001 gccggcgtcc gcctggggcg agcgatagaa cggcgagaac cagatggcgt cgactccgag
    12061 tgccttcaga tatggcagtc gagaccgcgc gcccggcagg tcgccgatcc cgttgccgtc
    12121 gccgtcggcg aaggaccgcg gatagatctg gtagatgacc gcatgccgcc accaggacgc
    12181 atcggcgcgg tcacgggcat ggatcggtcg agaatcgagg acggtcacgg tcatctattg
    12241 gatcgcaccg gcgcggctta gccaaaccgc gcggctcacg tccggtcagg ccgacgtcag
    12301 caggtgcgtc acctggtcca ccacccgcga ggcaccggcg atgtgccagc gccggtcgcg
    12361 atggtgaatg accgatccga cgcctccgac accgcggacc agcgccagcc cgggatacca
    12421 gtcccagtcg gccgtgccac attgcaggaa cgcaccggtc cgcccggtcg cgacggcggc
    12481 cagttcgctg gaggaagacc cgaacatccg caccgtcgcg gcgccccggg cggccgcgag
    12541 ccacggctcc aacagcgccg ggtcggccaa ccggcgggcg tgcaggtagg tcgccagcgc
    12601 accgtcggcg aggtcgtggc ccgcgagctt cggcaggtgg cggccgttga gggtcgtctc
    12661 cccctgcccg tgcgcatcga cgcggcccac ccaggtctcg tcgctcggtt cctggcggat
    12721 cgccccgacc aggctgccat cgctgtgccg caacgcgatt gcgctgcacc acggccccac
    12781 accgttggcg aagttgaagg tgccgtcgat cggatcgatg acccaggtcc gccccgatga
    12841 ggagggccag gtcgagccct cctcccccac gatcccgtcg tcgggccgta gcgcggacaa
    12901 ggccgcgacg atgtcggcct cggctcggtg gtccgccgcc gtcacgaagt ccgcggccga
    12961 ggtcttgcgc gtccgttcga ggggcccggc acgcaagtgc gcaccgatcg ccgcccctcg
    13021 acgcaccagc gcggcagcca ggtccccgtc gtcgtccggt gccccgagtc cctccagcca
    13081 ctcgtccgtg ccgggcagaa ccgctgcggg cgtcatgaca gcaacattaa cgggcgaggg
    13141 cgtgcccggc ggctgtgctc agccgccacc agggcccaga ccggaagagt cgcgccggct
    13201 cgcccggagt gaggaagcgc aggcggtatg ccgcgaacgc cagcccccgc ggcgcgtgcg
    13261 agccggtgag gggttcgccc cagacccggg cgcggagggc gtcgacctgc gcagccagac
    13321 cggtgcccgc ctcgccgagt tcagtgatcc cggcgcgcac ctcgcggatg aggtcctcgg
    13381 cgtcgagctc gccggccggt tcccaccccg agcgcggcgg cgactgcgcg gcccacggct
    13441 cgaacagcga catcgggggc acggggatct cgtaacggtc ggggtggtgc gcccggccgt
    13501 aggcgatgcg gtcgagcaca gccgccgccg gcacgaccac gtcgacgtcc tcgccgggtt
    13561 cgaccgcgaa ggtgcgcagg cccaaaaccg tgccgccccc ggtgattccg cgtccgggca
    13621 cgacgcagac gtatgccgcg agcaccgggc ccgaggcgac caaccggatg cccgcgtcgg
    13681 cgtccgccgt gcgcacgatc gtcgcgtagc gcttcaggtc gtcctgtgat tcggcatccg
    13741 ccagcacgat cccgctcatc gccggcgcca ccggaaggcg acgggttcac cggccaggcc
    13801 ctgcaacacc tcgcgctccc gcggatccag ccgccgcggc gcggaccgcg cgaagtcgta
    13861 ggcgacgagt gaggtctcgg cgcgggcata caactgctcg cccacctcgt cggggtcgcg
    13921 cacctcataa gccacgtcga aactggcgcc ggcgatcgcc gtgggccaca acatgatccg
    13981 caccggcgcg tgccggaaga ccagcggcgc gaggtattcg atctcgtggc gggcaacgag
    14041 cactccggag tccagcatcg aacggtcctc gtcgaaccac tccgcgaagg ccgccacccg
    14101 ggcgtcctcg aggaggcgga ggaactggac gttgttgacg tgcccgtaag cgtccatgtc
    14161 ggaccaccgc agggcaacat cgacactgcg gctcacgacc gcttgccctt gcctctcgag
    14221 cgcttcgggc cggtgcgcgg cgccccggcg ctctcgggtt gagccgcctg tgtcgtcgcg
    14281 tccgggtcgg ccgcagcgac gccgcgggtc tcgggtgcct tggcagcgcc gcgcggcggg
    14341 gcggcctcct tctcgctgac gtgccgcagc acgagcatgc tgcgcggcgc cacctcgacc
    14401 acgctcccgg ccgtgtgaat cgaatcgtcg gtttcggttg cgcctgtgtc gatctcgatc
    14461 ttccaggcca gtccgtagtc ggcgatgggc agggtgaacg gcagcatctc gtggtgggcg
    14521 ttgaagatca gcaggaagct gtcacccttg atgcgcatgc cacgcacatc gggctccggg
    14581 atcgcctgac cgttgaggaa gaccatgatc gagcgggcat gcccgttgcg ccagtccgat
    14641 tcactcatct gcgacccggc cggggtgaac cacgagatgt cgtgcacctc gctctcgccg
    14701 ccgtggtcag cgctgccggc gaagaaccgg cgccggcgga agacggggtt ctccttgcgc
    14761 agctgcagca cgcggcgggt gaagtcgagc aggtcgcgtt gatcggcgtc gaggtcccag
    14821 ttcatccagg cgatctcgtt gtcctggcag taggcgttgt tgttgccgcc ctgggtgcgg
    14881 ctgacctcgt caccgtgggc gagcatcgga acgccttggg agagaaggag agtcgtgatg
    14941 aagttctttt gctgccggat gcgcagcgcg cgaatgacgg gatcgtcggt cgggccctcg
    15001 acgccacagt tccacgaccg gttgtggctc tcgccgtcgc ggttgtcctc accgttggcg
    15061 gcgttgtgct tgtcgttgta ggagaccagg tcccgcagcg tgaagccgtc gtgggcgatg
    15121 acgaagttga tggaggcgat cggcttgcgg ccggaatgtt cgtagaggtc ggagctgccg
    15181 gtgatgcgtg aggcgaactc gccgatggtc gccggctcgc cgcgccagaa gtcacgcacg
    15241 gtgtcgcggt acttgccgtt ccattcggtc cacaggggcg ggaagttgcc gacctgataa
    15301 ccaccctcgc cgatgtccca cggctcggcg atgagtttga cctggctgac gaccgggtcc
    15361 tgctggatca ggtcgaagaa ggccgacagc ttgtcgacct cgtggaactg gcgggccagc
    15421 gtggcggcga ggtcgaagcg gaaaccgtcg acgtgcatct cggtgaccca gtaacgcaac
    15481 gagtccatga tcagttgcag cacatgggga ttgcgcatca acagcgagtt gccggtgccg
    15541 gtggtgtcgt agtagtgcgc cggattgctt tgcaccaggc ggtaatacgc gaggttgtcc
    15601 aggccgcgga acgcgagcgt cgggcccatg tggttgccct cggcggtgtg gttgtagacg
    15661 acgtcgagga tgacctcgat accggccgcg tgcagcgact tgaccatcga cttgaactcg
    15721 ggcacctgct caccccgggt gccgtaggcg gcatacccgt tgtgcggcgc aaagaacccg
    15781 atcgtgttgt aaccccagta gttcgacagg cccttgtcct gcaggtggaa gtcgtcgacg
    15841 aactgatgca ccggcatcag ctcgatggcg gtgacgccga gggagacgag gtgatcgatc
    15901 atcgccgggt gggcaatgcc ggcataggtg ccgcgcagcg cctcggggat gtcggggtgc
    15961 agcatcgtca tgcccttgac gtgcgcctcg tagatgacgc tctcgtggta ctcccgcttc
    16021 gggggccggt cctggtccca gtcgaagaag gggttgacga cgacggacag cagggtatgc
    16081 ccgaggctgt cgagcccgtt gagctcgcct ttggtgcccc ggtccacgtc ggtcatcgga
    16141 tagctgaaga gcgacgggtc accctccatg tgcccgtcga tcgccttggc gtaggggtcg
    16201 aggaggagtt tgtcggggtc gaagcgcagg cccagttccg gcaggtaggg cccgtggacc
    16261 cggaaaccgt aacgctgacc cggttgcaga cccggcaggt agctgtgcca gacgaatccg
    16321 tcgacctcct gcagcgggac gcgctcctcg gtgccgtcgt cgccgatgag gcacagctcg
    16381 accgctgtcg cgccttcgga gaagacggcg aagttgacgc ccgagccgtc gtaagtggcc
    16441 ccaagggggt agggcttacc cggccacaga tccatgctgt cctcaccgta ggctgtttgt
    16501 gaacctagaa cctatccgcc cgcgcacgcg gacggaaatc actgcaaggg agcagccatg
    16561 tccgagtcca cccgagtcgc cgtcgtcacc ggtgctgccc gcggtatcgg ggcagccacc
    16621 gcgatgcgcc tggccaagga cggcaacgcc gtcgccgtgc tcgatctgga cgagggtgcc
    16681 tgcgcgggca ccgtcgacgc catcaccgct gccggcggcc gggccttggc ggtcggcgtg
    16741 gacgtctcgg acgaggccca ggtccaggcc gccgtcaccc gcatcgccga ggagctcggc
    16801 gcccccgtca tcctcgtcaa caacgcgggc atcatccgcg acaacctgat cttcaagatg
    16861 acgacagctg actgggatgc cgtgatgggc gtgcacctgc gcggagcctt cctcatggtg
    16921 cgcgagtgcc agaagtacat gacgcaggag aagtacggcc gcatcgtcaa cctgtcctcc
    16981 tcctccgcgc agggcaaccg cggccaggcc aactactccg ccgccaaggc cggcctgcag
    17041 ggcttcacca agactctcgc catcgagctc ggccgtttcg gcgtcacggc caacgccgtc
    17101 gcccccgggt tcatccagac cgatatgacg gcggccaccg ccgagcgcat cggtatgccg
    17161 ttcgacgaat tcatcgccgc caccgccaag accatcccgg tcgcccgcgt cggccagccc
    17221 gacgacatcg ccgcgacgat cagcttcctg tgctccgagg aggccggctt cgtctccggt
    17281 caggtcatct acgtcgccgg tggcccgctc gactgacctg ccctgctctt ccgaccggtc
    17341 ccggcgtccc gtctagtggg accctggcca ggcttgcgcc gtcacggagc ctgatcgcgc
    17401 gcacggtcgt gtcgacgaag ctcgacagtt ccggggcata ccccatgccg ttcgcgatga
    17461 cgggcgggcc gccgacgcgg tacgcgggaa ggcgaaccgg ccaacgatga gcgtcaacgt
    17521 cgcgtctcct gtcgatgaac gggtgccact ggcgaacgtc gccgaggcgg tggtgtcgac
    17581 cggtctcggc cgaccgggcc gccaccgggg cccaagcaca gcaggcgatt ccattgaggc
    17641 aggaacacgc ggccgcgaga gatggccgcc cccatcggct cgtaaccggc tccgccgggc
    17701 agggcaaggg tcagcagccg tcgtcccgag ccgatcgaga atccgagaag cgccggcgtg
    17761 tcgagaacgg ctccagccgg caccccgatc aggacgactc cgccgtcgca gaccatcccg
    17821 ggactgagcc atgcctcgtt ggtgaagcgc cagcgaatta ccccggtcgc ggcatccagg
    17881 gcgaccaggt cggtttcggc gttcgccaac accgtggatc cagaccaggc aactgcccgc
    17941 cacaccgatt tgcccagctc ggtctgccag agggtggtgc cgtcggcgac gctgacggcg
    18001 cgcaccgcgc ccgaatagct gccaccggtg ctcccgacaa atgcgcgacc ggaggcccgg
    18061 tccacggcga tgccgaaacc atccatggtc cagccgttcg tgccgggcaa ccggttctgc
    18121 cacgcaacct ttccgtcgga caggttcagt gccgtcaccc gcgagttgcc ctcgttcccc
    18181 gcgatgacca ggccgccggc tgcactcggc gagccgaaca tccccgccca gtactccggg
    18241 tcgacccacc cccagcgccg ccctcccgtc ctgaggtcga aggcctggac gcggtcctcg
    18301 ctgcccacga gcaccgtgct cccggcgatg ctcggtgacg cgaaacccga tctggccagc
    18361 cgacgcttcc acaccagtgc tccggtgcgg gcatcgatgg cgccaagatg cgaatccgag
    18421 ccgtcgagtc ctgtcgccac aaccagccca tgggcgtaga cgggtgtcgt ggggaccact
    18481 gcgccgatcg agcgggacca gacgcgagtg ccggtctgtg cgtcataccg aacaaccgtc
    18541 cggccgccga ggaaaactcc gttgcccacg acggtggggt ctccggtggc gggcgcccac
    18601 ccggactccg ccaccggacg ctgccacagc tgacccaggg agccgacggt cgccaccgtt
    18661 accgtcgttt cggtgcgcgc ccagcccgag tggccgctgt ccgccatgaa ctggggatag
    18721 tcgtcggcag tggcgtcggg gcctggccag gccgccgcct ccgccgtcgt gctcagcggg
    18781 gtcgtgactc ccgccgccag gcccgcggcg agagccgcgc gccgcgatac gtcactcatg
    18841 tgcgcctccc ctgctcagcc gcccgcgcgg cctgccgtgg attcgacatt aggcggtgtc
    18901 ggcctcgcct gcaacggttc gtggagagtc acaggcctgc gaccagcgtc actcccccgt
    18961 ccacgacaag ggtctggcca gtgatccacc ccgcatccga ggacaacagg aagctgaccg
    19021 cccccgcgat gtcctccggc acacccagcc ggttgagggg gtagggcgcg gcgacctgct
    19081 cctcccggcc gtcatacagg gcctcggcga acttggtctt cacgacggcg ggggcgacgg
    19141 cgttgacgcg caccgccggc cccaactggt agcccaactc gacggtgagg tggatcagcg
    19201 cggccttcga cacgccatac atcccgatca cgccggaggc accgatcccg gcgaccgagg
    19261 cgacgttgac gatggcgccg cggccgccgt cggcgagccc ggccgccacc actttctggg
    19321 tccacgagag cgcggccacg acattggtat cgaggatctt gcgggccgcg tcgagtgggg
    19381 tgtcgaggat cgggccgtat gccgggttga tgccggtgtt gttgaccatc aggtcgagc
//
LOCUS       DAOPJG010000039        19374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2985_length_19374_cov_8.092914, whole genome shotgun sequence.
ACCESSION   DAOPJG010000039 DAOPJG010000000
VERSION     DAOPJG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 19374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19374
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_19374_cov_8.092914"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PKU75_05530"
     CDS             <1..849
                     /locus_tag="PKU75_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF80374.1"
                     /translation="MADAAAAHPDLVTGTAFVYRRQPAVAAIKNLVGQRLGEVSHFNG
                     RYWCDYANSPLTPMAWRYQGGPGTGALNDIGSHLIDLAEFVCGPMTHVSGASFTTLVK
                     ERFVAKGTTYGHSRAELTEEKKAVENDDVATFTATFANGAVGTFSISRIAPGHANSMA
                     FDVFAQHGAAKWDMDRPAEFSVVLNESDDALAAYNGYNQVLVGPAHPYLKGGVPMDFP
                     GVSYGVGDLFGFQARAFLEQVAGIEGGLPPVASFADGLRDLEIINAVISSAAAGGATV
                     ELPSHH"
     gene            938..1924
                     /locus_tag="PKU75_05535"
     CDS             938..1924
                     /locus_tag="PKU75_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPF80375.1"
                     /translation="MYIGVYNACLGNKSLGEALDVIKGLGLNSVEINSGGFLPPIHLP
                     VEELKASEDARQAYLGEFESRGLTLTALNCNGNPLFPTAGFPHAQDLVDSIELASLLG
                     VKRLVTMSGAPAAEAGGQHMAWNVLPWWSPLLDVRDYQWEVAVPFWKDIQARAADADV
                     KVAIEMHPGNVVYNPATMHRLAEEINATHVGAEMDPSHLFWQGIDPVLALKDLGSLVY
                     CSAAKDTRINEAAKINGVLDDRFTRVAEGEPGYLPLGGGTTLSGWPSNSSWDFVAIGK
                     GHDVAFWTEFLRALQEIDPDLPCNIEHEDAELGQLEGLEYAARNMLAAEQGL"
     gene            1921..3003
                     /locus_tag="PKU75_05540"
     CDS             1921..3003
                     /locus_tag="PKU75_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF80376.1"
                     /translation="MTVLPEVEPLRIGLLGAARIAPLSIVSPAQATGARLVAVAARDR
                     SRAADFAAAHGVERVVESYDALLADPEVEAVYNPLANGLHAPWNARIAAAGKHVLAEK
                     PSAANAAEARDVADAVRAAGVVFMEAFHFPYHPLFQRTCELIGQGAIGEVRHIDVPLL
                     MPDPGADDPRWRMDLAGGSTMDLGCYSLSCLALLGEQFCGGSFTINTAEAAEREGHPG
                     VDSHLFVAGEFPSGATGSGGSDMDAKDWSFTLVVTGSEGEIVVPSFPLPHEDDRLILR
                     HTAAAPRSRRLPDEAADLTSERDGDVVEHLGNRSSYTYQLEAFAAAVRSGAPVVTDAE
                     FAVRNMTLVDSAYVAAGLPVRESTTP"
     gene            complement(3165..3974)
                     /locus_tag="PKU75_05545"
     CDS             complement(3165..3974)
                     /locus_tag="PKU75_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPF80377.1"
                     /translation="MAPAGSFTLAASSEMLYRDLPVAERIRRIHEAGFAVEIWDWRGH
                     DIDALGALHDEGVTFTSMTGYTTGTLGDADGADELLRTAAESIPVAKRIGAWGLNLHG
                     TGLDDKGLPVKALAPGEVASEQMWATARHTLQRIADLGEREGVTFLLENLNLVVDHPG
                     TPFARAEDTLALVSGIDSPSLKLNLDLYHAQIGEGNLIELCRAALPWIGEIQVADVPG
                     RCEPGTGEINYSGVARALREMGYAGVVGLEAFASGDSDLALQRFREAFTVT"
     gene            complement(3976..5478)
                     /locus_tag="PKU75_05550"
     CDS             complement(3976..5478)
                     /locus_tag="PKU75_05550"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HPF80378.1"
                     /translation="MSTPTRITHLINGAAWEGTAERTSPVYNPATGAQTGVLDLASTD
                     LVDEIVRGAKAAWENEWQNASLTKRTQVLFTFRELLNAKKEEIGAIITSEHGKVLSDA
                     VGEVTRGLEVAEFACGIPQLLKGGFSENVSTRVDVYSIRQSLGVVAVISPFNFPAMVP
                     LWFVPIAIACGNAVVIKPSEKDPSAVNAVAALWKEAGLPDGVLNVVHGDKEAVDALLT
                     HPDVKAVSFVGSTPIAKYVYETGTAHGKRVQALGGAKNHMLVLPDADLDLAADAAVNA
                     GFGSAGERCMAISALLAVEPIADDLISKITDRMGKLVTGDGTRGSDMGPLVTREHADK
                     VTSYLDKGVDAGATLVVDGREGDVDADGEGFFLKPTLFDNVTPGMPVYDDEIFGPVLS
                     VVRVQSYDEGLKLINDNPYGNGTAIFTNDGGAARRFQNEVEVGMVGINVPIPVPMAYF
                     SFGGWKNSLFGDTHAHGMEGVHFFTRGKVVTSRWLDPSHGGLNLGFPQNT"
     gene            complement(5546..6436)
                     /locus_tag="PKU75_05555"
     CDS             complement(5546..6436)
                     /locus_tag="PKU75_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPF80379.1"
                     /translation="MKIALDPYMFREVTTLETLPGVVADLGYEWIELSPREDFTPFFT
                     HPRVNDSGAKAFRKALQGAGVGVSSVLPLYTWSGPDEDARQAAVRYWKRAIQLTSLLG
                     VDTMNSEFNGNPRQADVCEAQFWRSMEELLPVFEKEGIRLVLEPHPDDFEEDGKRAID
                     LIRALNSDNVSFLYCTPHTFHQGNDVDGILDKAGALTTMCHLADAYDHTASSGLRYIV
                     NPPGSQVTVHQHLDMGQGEVDFGRFFAALERNGFDGILTACVFAWEDRAEESSRFMKR
                     AIEEHLSAWASPPKLESPTA"
     gene            complement(6433..7464)
                     /locus_tag="PKU75_05560"
     CDS             complement(6433..7464)
                     /locus_tag="PKU75_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF80380.1"
                     /translation="MAENELRVGVIGVGMMGADHADRLVHRVAGARLVAVSDPDTARA
                     AALAGRYDGVRSVDDPLALVADDEVDAVVIASPGFVHEEQVLACIAAGKYVLCEKPLT
                     MDSESSLRVLRAEREAGKELIQVGFMRRFDPQYAQLQQLLASGDLGRTLLLHNTHRNK
                     SVPNPEFRSEMIVRDSLVHEVDVARFMFGQEITTIQVLSPAPTSHACEGVVDPQVALF
                     TMAGGGVVSNEVFVNCQVGYEVRCEAVSERGTAIAGRPQSGLYTTRVGEPAGSWGGTV
                     DPDFIARFGLAYDLEFQAWVDATRRGEVVGATSWDGYASTAVCEAGMQSLASGQPVAV
                     TLVDKETLT"
     gene            complement(7473..8366)
                     /locus_tag="PKU75_05565"
     CDS             complement(7473..8366)
                     /locus_tag="PKU75_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF80381.1"
                     /translation="MTTATATGTRLLELDEVGKSYGNVHALRGVNISVSPGEVTCVLG
                     DNGAGKSTLIKIIAGLHQHTEGVVRMDGEEIRFNSPREAQAKGIATVYQDLALAPLMS
                     VWRNFFLGNEMKKGFGLDIDAMKRISGEELQKMGIVIPDLDRPVGALSGGQRQCIAIA
                     RAIYFGARVVILDEPTAALGVKQSGVVLKYVTKMRDSGLGVIFITHNPHHAYMVGNHF
                     VILKLGRLVLDAHRDEITLDQLTAEMAGGQELAELSHELRASEADGTADAAAADAVEA
                     EVDHSLFQRGHGATSVEEHKE"
     gene            complement(8363..9400)
                     /locus_tag="PKU75_05570"
     CDS             complement(8363..9400)
                     /locus_tag="PKU75_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF80382.1"
                     /translation="MSTASTATASDDRVRTRSAASTFFGMPAVGALIGAAVLVALFLA
                     VAPAFRSVGNIGTILYSASTIGIMAVFVALLMIGGEFDLSTGVAVTTAGLTAAHSAWY
                     FNLNVWVGVLISLLFALAIGAFNGWLLHVTKLPSFLVTLGTYFVFWGANLAMTRIVTG
                     GVSSNSISDMEGFNSAHAVFASEFSIGSVNIKIPIIYWILLTVAVSYLLLRTKYGNWI
                     LAAGGDSAAARAVGVPVVATKIALFMGTGFAAWLLGMHTLFEYNTLQSGTGPGYEFIY
                     IIAAVIGGCLLTGGYGSIVGASIGALIYGMAVLGISYAGWDVDWLRTFLGVMLIAAVL
                     INNYVRKRADA"
     gene            complement(9408..10403)
                     /locus_tag="PKU75_05575"
     CDS             complement(9408..10403)
                     /locus_tag="PKU75_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPF80383.1"
                     /translation="MIQRKKLGVAAVSLSLMGSLVACSSTGGRQAVEQKVGADGAKAK
                     TMTVTMVTHQAPGDTFWDIIRKGAEQAATDVGVNLEYTNDPDATKQAQLIQAAIDKKV
                     DGIAATNPNTGAIGPVVKKAIEGGIAVSMFNAGGPDAMDLGVIGYFGQAETDAGVAVG
                     ERLAKDGAKNAICVIQEQGQQQLEERCKGVEDGAGGAVKVARVYVDGRNDAAVVTSLQ
                     AQLTQDKTIDWVVTLGAPFALDAVKSVEGAGSAAKIGTFDTNPALVDAVKGGKVQWAI
                     DQQPFLQGYLAVTSLYLYKINGNVIGGGQRVPTGPAFIDSTNIEAVAKFAANGTR"
     gene            complement(10513..11445)
                     /gene="iolB"
                     /locus_tag="PKU75_05580"
     CDS             complement(10513..11445)
                     /gene="iolB"
                     /locus_tag="PKU75_05580"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HPF80384.1"
                     /translation="MTTGTGRDNEHWVFPKGAAAQGDYATVIDAGIDGWQHTMLRVAE
                     ISEGASVTHDSGVEETIVVPLNGSVRVEVTDEDGTAHDIVLAGRAHVFASATDVAYAG
                     RGAVVRVTAEGGPARVALTGAPATKLAAPKPFRHIRADEVPVEIRGAGTSSREVRSFG
                     LPEVLDADSILVCEVITPAGNWSSWPPHKHDTETDEETQLEEIYYFELQPTDPRGTDP
                     VGYQRVYGSNEQPERPIDVVAEVRSGDVVLVPYGWHGPAMAAPTADLYYLNVMAGPGA
                     ERAWRICMDPAHVWVRDAWSDLPPDPRLPFTAGR"
     gene            complement(11442..12341)
                     /locus_tag="PKU75_05585"
     CDS             complement(11442..12341)
                     /locus_tag="PKU75_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPF80385.1"
                     /translation="MTAAATPLTMAGVTEIRVREPERIAAAWATRRRRERVGADGRLL
                     IVAADHPARGALGVRTDPLAMASRAELLERLVSALSRPGVDGVLGTPDVLDDLVLMGA
                     LEGKLAIGSMNRGGLQGACFELDDRFTAYTAAALQQRGLDGGKMLTRIALGNAGTAPT
                     LEASARAIGELAAYGLMAMIEPFMSEVRDGRVHNLLDPDSTIRSIHIASGLGDSSAHT
                     WLKLPVVDDLERVMEATTLPTLLLGGDPQGRADETYERWAHALTIPPVRGLVVGRALL
                     FPPDGDVAAAVDIAARLVHQEAP"
     gene            complement(12338..13306)
                     /gene="iolC"
                     /locus_tag="PKU75_05590"
     CDS             complement(12338..13306)
                     /gene="iolC"
                     /locus_tag="PKU75_05590"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HPF80386.1"
                     /translation="MADGQTFDVISLGRSGVDIYPLDHGVGLEDVSTFRKFLGGSATN
                     VAVAAARHGHHVALVTKVGNDPFGRFVHAESARLGIDPRFISTIDGDGPPTPVTFCEI
                     FPPDNFPLYFYRYPAAPDLLLTEADLPLDEIASAPVFWATVTGLSQEPSRATHFAAWQ
                     RRGRRSHTVLDLDYRPMFWDDPAQASTQVARALEHVSVAVGNAEECFVAVGERDPQRA
                     ADALLERGLDLAIVKQGPKGVLAATAEERIEVAPHWVEVVNGLGAGDAFGGGLIHGLL
                     SGWDLRRTVEFANVAGAIVASRLECSTAMPTTAEVEAALDGRRSES"
     gene            complement(13311..14339)
                     /locus_tag="PKU75_05595"
     CDS             complement(13311..14339)
                     /locus_tag="PKU75_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF80387.1"
                     /translation="MKLAVAGVGRIGAFHARTLKDVAGVDTIVVADLNHEAASSVAAE
                     IGAEVVADPLDLVHQGIDGLVIATATDGHAPLLRAALTAGIPVFCEKPVAGTLGETIE
                     LAELEAKTGTLAHIGFQRRFDAAYRRARAAVASGEIGTIHAIRATTHDQAPPPRAYVK
                     VSGGIFHDCNIHDFDIIRFVSGAEPTTVYATGANKGDPMFTEEGDVDTAAAIITLDDG
                     TLVTVTATRYNGAGHDVRMEVTGSGGAVAVGLDDSLALRSLEDGVQFPAGPQCWSFFE
                     RFHDAYLAELSAFAGMVATTSPSPCTVRDALEASRIAHACDLSRAQARLVHMSEIPGL
                     DAPIADGT"
     gene            complement(14507..15688)
                     /locus_tag="PKU75_05600"
     CDS             complement(14507..15688)
                     /locus_tag="PKU75_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF80388.1"
                     /translation="MAQMDSTGRRTIGVGVIGAGWLGDVHARAWARLRHHYADLGVTP
                     VFVAVADSVPAAREAAVSKHGFATAYDDWRDLLADPAVEAVSVTAPNALHQEIGVAVA
                     QAGKHLWIEKPVGLSAADAQAVADAVADAGVQGTVGFNYRAIPAMAKLRDLVAEGAIG
                     APTHARVRLLTDYAAHPLGTLTWRYSLASGGHGVLGDLASHAVDLTRYVLGDIESLVA
                     QTAIFIAQRPTIVPGAMTYGHGLGDPDAPKGQVENEDYVQAMMRMRSGVLVALESSRV
                     AVGEQNNYGIEVHGSQGLVEWDFRAPGELRLSTGTNYADQPSTRLLVGPGAGDHGRFQ
                     PGSSIPTSYDDTKVIELAGFVRSILSGQPEGPVLGDAVASAHALDAMVNSAAGAGWVT
                     L"
     gene            complement(15705..16670)
                     /locus_tag="PKU75_05605"
     CDS             complement(15705..16670)
                     /locus_tag="PKU75_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HPF80389.1"
                     /translation="MSHRVLLTGGTGFVGGNVASVLAGRGADVLCAVRRDPGPDFPWA
                     WRLTDLADPGDIAAALADHESTAVLHLAIDNDLQGLYRDRKAGFDAYVGMTRRLVDAC
                     NAAGARFGYLSTDWVFDGFGHDVDEDEPVNPLNLYGLYKALSEQVVIDRADHGFVARV
                     GGVQGRHLTQPSAPRAQDCGFGYFVLSLVDSLSAGQQFSVWQDPAINNVASPVTAAEI
                     GARLLLALDREADGILHVVGADAVTRLELAQAAAEVFDLDASLITTSPVPEGQMLPAP
                     MPADTSLATPRSDEVLGVKPYGIRDQLRALRREAETGLPQPLTPA"
     gene            complement(16667..17671)
                     /gene="iolG"
                     /locus_tag="PKU75_05610"
     CDS             complement(16667..17671)
                     /gene="iolG"
                     /locus_tag="PKU75_05610"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HPF80390.1"
                     /translation="MGVVGTGRIGRMHAGLLLREVPGTTVTAVADAVPEVAQQVAGDL
                     AVAALGIDELLASPEVDAVAICTSTDTHVDLTIKAAEAGKAILCEKPVSLDLAEVDRA
                     LAAVEAAGVPFMVGFNRRFDPGHAAVAAAVRTGQIGELHLVRITSRDPAPPPPEYVAV
                     SGGIFLDMMIHDFDMARYVVGSEVVEVYARGAVRVDPRIGDAGDVDTAVVVLTHEDGT
                     ITTIDNSREAVYGYDQRVEAFGSGGMALSDNPLRHAAWVRTPDTTAAEPLPWFFLDRY
                     LPSYRLEWAAFADYVRDGGASPVGTTDGREPVRIGIAATRSLKEGRPVRLDEISSDPA
                     "
     gene            17947..18684
                     /locus_tag="PKU75_05615"
     CDS             17947..18684
                     /locus_tag="PKU75_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPF80391.1"
                     /translation="MDTIPVVIDRASPVPLYHQLAEQLTAAIESGRLGPGDPFENELA
                     LADRLDLSRPTVRRAIAELVQRGLLVRRRGVGTTVASPMVHRRDELTSLYDDMERRGQ
                     HPVTRVLRLEHDVRDPVAAEALGVPATTPLVFIERLRSAGDVPAALMHNWLPPAYADI
                     TFADLEGGSLYGWLKARGIKPVVAHQVIGARRPTAGERKLLRLAAGDPLLTMSRRAYA
                     ADGSAVEYGDHCYRFDQYAFDVTVHER"
     gene            complement(18846..19259)
                     /locus_tag="PKU75_05620"
     CDS             complement(18846..19259)
                     /locus_tag="PKU75_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPF80392.1"
                     /translation="MTVAVAHQTSRYSQAVLTEAARQAAQRGEELIVISVEKAADEDH
                     RAALEASVGDDLAKAFAAASVADVSWSLDLEAASAEIDDTAETILDAVKRTGATLLVI
                     GARRRSPMGKALLGSVTQTLILDSPVPVLVVKAPN"
BASE COUNT         3072 a         6524 c         6657 g         3121 t
ORIGIN      
        1 atggccgatg cagcggcagc gcacccggac ctggtcaccg gcaccgcctt cgtctatcgc
       61 cgccagccgg ccgtggccgc gatcaagaac ctcgtcgggc agcgtctcgg ggaggtgtcc
      121 cacttcaacg gccgctactg gtgcgactac gccaacagcc ccctgacgcc gatggcctgg
      181 cgttaccagg gcggcccggg cacgggcgcg ctcaacgaca tcggctccca cctgatcgac
      241 ctcgccgaat tcgtctgcgg gccgatgacc cacgtctccg gcgcctcctt caccacgctg
      301 gtcaaggagc gtttcgtcgc caagggcacg acatacggtc actcccgcgc cgagctgacg
      361 gaggagaaga aggccgtcga gaacgacgac gttgcgacct tcacggcgac cttcgccaat
      421 ggggctgtcg gcaccttctc catctcgcgc atcgcccccg ggcacgccaa ctcgatggcg
      481 ttcgacgtct tcgcccagca cggcgcggcg aaatgggaca tggaccggcc cgccgaattc
      541 tccgtggtgc tcaacgaatc cgacgacgcg ctcgcggcat acaacggcta caaccaggtc
      601 ctcgtcgggc cggcccaccc ctacctcaag ggtggcgtgc cgatggactt ccccggggtg
      661 tcctatggcg tgggtgacct cttcggtttc caggcccgcg ccttcctcga gcaggtcgcc
      721 ggcatcgagg gcggcctgcc tcccgtcgcg agcttcgccg acgggctgcg cgacctcgag
      781 atcatcaacg ccgtcatctc ctccgccgcc gccggcggcg ccaccgtcga actcccttcc
      841 caccactgac ccccaccaag tgtcctcgct gcgctgcggt acttcgcggg gaccacagtc
      901 gcatcgatag cccacgaaga gccccaagga gcaagtcatg tacatcggtg tctacaacgc
      961 gtgcctcggc aacaagtccc tcggtgaggc cctcgacgtc atcaaaggcc tcggcctcaa
     1021 cagcgtcgag atcaactccg gcggcttcct cccgccgatc cacctgcccg tcgaggagtt
     1081 gaaggccagc gaggacgccc gccaggccta cctcggggag ttcgaatccc gcggcctgac
     1141 cctgacggcg ttgaactgca acggcaatcc gctcttcccg accgccggct tcccgcacgc
     1201 ccaggacctc gtcgactcca tcgagctggc gtccctgctc ggcgtgaagc gcctggtgac
     1261 catgtccggt gccccggccg ccgaggccgg cggccagcac atggcgtgga atgtgttgcc
     1321 ctggtggagc ccgctgctcg acgtgcgcga ctaccagtgg gaggtcgccg tccccttctg
     1381 gaaggacatc caggcccgcg ctgcggacgc cgacgtcaaa gtcgccatcg agatgcaccc
     1441 gggcaatgtc gtctacaacc ccgcgacgat gcaccgcctc gccgaagaga tcaacgccac
     1501 gcacgtcggt gccgagatgg acccgagcca cctcttctgg cagggcatcg atcccgttct
     1561 cgcgctgaag gatctgggct cgctcgtcta ctgctcggcc gccaaggaca cccgcatcaa
     1621 cgaggccgcc aagatcaacg gcgtcctcga cgatcgcttc acccgcgtcg ccgagggcga
     1681 gccgggctac ctcccgctcg gcggcggcac caccctctcg ggctggccga gcaactcctc
     1741 ctgggacttc gtcgccatcg gcaagggcca tgacgtcgcc ttctggaccg agttcctgcg
     1801 cgcgctgcag gagatcgacc cggatctgcc ctgcaatatc gagcacgagg acgccgagct
     1861 cggtcagctc gagggcctgg agtatgccgc caggaacatg cttgccgcgg agcaaggcct
     1921 gtgacggtcc tccccgaggt cgagccgctg cgcatcggtc tgctcggtgc cgcccggatc
     1981 gcgccgctgt cgatcgtcag cccggcgcag gcgacgggcg cgcgcctggt ggcggtggct
     2041 gcgcgggatc gttctcgcgc agccgacttc gccgccgccc atggcgtcga gcgcgtcgtg
     2101 gagtcgtatg acgcgctgct cgccgaccct gaggtcgaag ccgtgtacaa cccgctcgcc
     2161 aacgggctgc acgctccgtg gaatgcgcgc atcgcggcgg ccggcaagca cgtcctggcc
     2221 gagaagccgt cggccgccaa cgcggccgag gcgcgcgacg tggccgacgc cgtgcgggcc
     2281 gccggggtgg tgttcatgga ggcgttccac ttcccctatc acccgctctt ccagcgaacg
     2341 tgcgagctca tcgggcaggg cgccatcggc gaggtccggc acatcgacgt gccgctgctc
     2401 atgcccgacc ccggcgccga cgacccccgc tggcgaatgg acctggccgg cggctcgacg
     2461 atggacctcg gctgttactc cctctcctgc ctcgccctcc tcggcgagca gttctgcggc
     2521 ggctcgttca cgatcaacac ggccgaagcc gccgagcgcg agggtcatcc cggcgtcgac
     2581 tcccacctct tcgtcgctgg cgagttcccc tcgggggcaa cgggttccgg cggctccgat
     2641 atggatgcca aggactggtc gttcaccctt gtggtcaccg gcagcgaagg cgagatcgtc
     2701 gtgccgagct tccccctacc gcacgaggac gaccggctca tcctgcggca tacggctgca
     2761 gcgccccgct cccgacgcct tcccgacgaa gccgccgacc tgaccagtga acgcgacggc
     2821 gacgtcgtcg agcacctggg caaccggtcg agctacacct accaactgga ggcgttcgcg
     2881 gcagcggtgc gctccggcgc ccctgtcgtc accgacgccg agttcgccgt gcgcaatatg
     2941 accctggtcg actccgcgta tgtcgcggcc ggcctcccgg tgcgtgagag cacaactccc
     3001 tagtcacccc acgaagtgtc ctcgctgtcg tcgaggtcac cgcaccaatc ggcggcagcg
     3061 tcattgtcga aggggcctgt ctgccacgcg ctcacggcga tcactctcgc cgatggccgt
     3121 gggcgacagg gcgatcagtc ctcatcccca cgccacctcg agagtcaggt gaccgtgaag
     3181 gcctcgcgga agcgctggag ggcgaggtcg gagtcaccgg aggcgaaggc ttccaggccg
     3241 accacaccgg cataccccat ctcgcgcaac gcgcgggcga ccccgctgta gttgatctcg
     3301 ccggtgcccg gctcgcagcg gccggggacg tccgcgacct ggatctcacc gatccacggc
     3361 agggcggccc ggcacagttc gatcaggttc ccctcgccga tctgcgcgtg atagaggtcc
     3421 aggttgagct tcaggctcgg gctgtcgatg ccgctcacca gggcgagggt gtcctcggcg
     3481 cgcgcgaagg gcgtgcccgg gtggtcgacg accaggttga ggttctccag gaggaaggtg
     3541 acgccctccc gctcgccgag gtcggcgatg cgctggaggg tatgccgcgc ggtcgcccac
     3601 atctgctcgc tcgccacctc gccgggcgcc aatgccttga cgggcaggcc cttgtcgtcg
     3661 agacccgtgc cgtgcaggtt gagcccccac gcaccgatgc gcttggcgac cgggatcgac
     3721 tcggccgccg tgcgcagcag ttcgtcagca ccatcagcat cgcccagggt gccggtcgtg
     3781 tagccggtca tcgacgtgaa ggtgacgccc tcgtcgtgga gggcccccag cgcgtcgatg
     3841 tcgtggcccc gccagtccca gatctcgacg gcgaagccgg cctcgtgaat gcgtcgaatg
     3901 cgctctgcca caggcagatc gcgatagagc atctcgctgc tcgccgccag cgtgaacgat
     3961 ccggccgggg ccatgtcagg tgttctgcgg gaaaccgagg ttcaggccgc cgtggctcgg
     4021 gtcgagccag cgggaggtga cgaccttgcc gcgggtgaag aagtgcacgc cttccatgcc
     4081 gtgggcgtgg gtgtcgccga agaggctgtt cttccagccg ccgaaggaga agtaggccat
     4141 cggcaccggg atcggcacgt tgatgcccac catgccgacc tcgacctcgt tctggaagcg
     4201 acgggcggcg ccgccgtcat tggtgaagat cgccgtgccg ttgccgtagg ggttgtcgtt
     4261 gatcagcttc agtccctcgt cgtaggactg cacgcgcacc acggacagca ccgggccgaa
     4321 gatctcgtcg tcgtagaccg gcataccggg cgtgacattg tcgaagagcg tcggcttgag
     4381 gaagaaaccc tcgccgtcgg cgtcgacgtc tccctcgcgc ccgtccacga cgagcgtcgc
     4441 tcccgcgtcg acgcccttgt cgagatagga cgtgaccttg tcggcgtgct cgcgcgtcac
     4501 gagcggaccc atgtcgctgc cgcgggtgcc gtcgcccgtg acgagcttgc ccatccggtc
     4561 ggtgatcttg gagatcagat cgtcggcgat cggttcgacg gcgagcaggg cggagatggc
     4621 catgcaccgc tcgcccgcgg agccgaagcc ggcgttgacg gcggcgtcgg cggccaggtc
     4681 gaggtcggcg tcggggagaa ccagcatgtg gttcttcgcg ccgccgaggg cctgcacgcg
     4741 cttgccgtgg gcggtgccgg tctcgtagac atacttggcg atcggcgtgg agccgacgaa
     4801 ggagacggcc ttgacgtcgg ggtgggtcag gagcgcgtcg accgcctcct tgtcgccgtg
     4861 cacgacgttg agcacgccgt cgggcaggcc ggcctctttc cacagtgccg caacggcatt
     4921 caccgccgag gggtccttct cggacggctt gatgacgacc gcgttgccgc aggcgatggc
     4981 gatgggcacg aaccacagcg gcaccatcgc cgggaagttg aagggactga tcaccgcgac
     5041 gacgccgagg ctctggcgga tcgagtagac gtcgacccgg gtcgagacgt tctcggagaa
     5101 gccgcccttg agcagctgcg ggatgccgca cgcgaactcc gcgacctcga ggccacgcgt
     5161 cacttcgccc accgcgtcgg agagcacctt gccatgctcc gacgtgatga ttgcgccgat
     5221 ctcctccttc ttggcgttga gcagttcacg gaacgtgaag agcacctgcg tgcgcttggt
     5281 caagctggcg ttctgccatt cgttctccca ggcggccttc gcaccccgca cgatctcgtc
     5341 gacgaggtcc gtcgaggcga ggtccaggac gccggtctgg gcgccggtgg ccgggttgta
     5401 gacgggggag gttcgctcgg cggtgccctc ccaggccgcg ccgttgatca ggtgggtgat
     5461 gcgagtgggc gtgctcatgg ttgctcctcc ggctgctcaa atgttgttac atactaacat
     5521 acaccgcatt cggtatgcgg tcaggtcacg cggtgggtga ctccagtttg ggtgggctcg
     5581 cccaggcgct caagtgctcc tcgatcgccc gcttcatgaa tcggctggac tcctcggcgc
     5641 ggtcctccca tgcgaagaca cacgcggtga gtatgccgtc gaagccgttg cgctccagcg
     5701 cggcgaagaa gcgaccgaag tcgacttccc cttgtcccat gtcgaggtgc tggtggaccg
     5761 tgacctggga gccgggcggg ttgacgatat agcgcaggcc ggaggatgcc gtgtggtcgt
     5821 aggcgtcggc gaggtggcac atggtcgtca gggcgccggc cttgtcgagg atgccgtcga
     5881 cgtcgttgcc ctgatggaag gtgtgcggcg tgcaatagag gaaggagacg ttgtcgctgt
     5941 tgagcgcgcg gatcaggtcg atggcccgct tgccgtcctc ttcgaagtcg tcggggtgcg
     6001 gctcgagcac gagccggatc ccctccttct cgaagacggg cagcaactcc tccatcgagc
     6061 gccagaactg cgcctcgcag acgtccgcct gccgcgggtt gccgttgaac tccgagttca
     6121 tcgtgtccac cccgagcagc gacgtcagct ggatcgcgcg cttccaatag cgcacggctg
     6181 cctgccgggc gtcctcgtcc gggcccgacc acgtgtagag gggcagcacc gacgagacgc
     6241 cgaccccggc cccctgcaac gcttttcgga aggccttcgc gccggagtcg ttcacgcgcg
     6301 ggtgcgtgaa gaagggcgtg aaatcctcac ggggcgacag ttcgatccac tcgtaaccga
     6361 ggtcggcgac gacccccggg agcgtctcca gggtggtgac ctcacggaac atataggggt
     6421 cgagggcgat cttcacgtca gcgtctcctt gtccacgagg gtgaccgcca ccggctgacc
     6481 ggaggcgagc gactgcatac cggcctcgca cacggcggtg gaggcatagc cgtcccacga
     6541 ggtggcgccc accacctcgc cgcggcgggt ggcatcgacc caagcctgga actccaagtc
     6601 ataggcgagg ccgaagcgag caatgaagtc cgggtccact gtgccgcccc aggatccggc
     6661 cggctcaccg acccgtgtgg tgtagaggcc cgactgcggc cggcccgcga tggccgtgcc
     6721 gcgctccgag accgcctcgc agcgcacctc atacccgacc tggcagttga cgaagacctc
     6781 gttggacacg acgccaccgc ccgccatggt gaagagcgcg acctgcgggt cgacgacgcc
     6841 ttcgcaggcg tggctcgtcg gcgcagggga gagcacctgg atggtggtga tctcctggcc
     6901 gaacatgaag cgcgcgacgt cgacctcgtg gaccagcgag tcgcgcacga tcatctccga
     6961 gcggaactcg gggttgggaa ccgatttgtt ccggtgcgtg ttgtgcaaca acagcgttcg
     7021 gcccagatcg ccggacgcga gcagctgctg cagctgcgca tactgcgggt cgaagcggcg
     7081 catgaagccg acctggatga gttccttgcc ggcctcgcgc tcggcgcgca gcacccgcag
     7141 ggacgactcg ctgtccatcg tcagcggctt ctcacacagg acatatttgc ccgccgcgat
     7201 gcacgcgagg acttgctcct cgtggacgaa gccgggggag gcgatgacca ccgcatccac
     7261 ctcgtcgtcg gcgacgagcg ccagcggatc gtcgaccgag cgcaccccgt cataccgccc
     7321 ggccagggcc gcggctcggg ccgtgtcggg atccgacacg gccacgagcc gggctccggc
     7381 gacgcggtgg acgagacggt cggcgtggtc ggcgcccatc atgccgacac cgatcactcc
     7441 gacgcgcagt tcgttctcag ccacggtggt tcctactcct tgtgctcttc gacagatgtc
     7501 gcaccgtgcc cacgctggaa gagcgagtgg tcgacctcgg cctcgactgc gtcggccgcc
     7561 gcagcgtccg cggtgccgtc ggcctcgctc gcacgcagct cgtggctgag ctcggcgagt
     7621 tcctggccac cggccatctc cgccgtgagc tggtcgaggg tgatctcgtc gcggtgggcg
     7681 tcgaggacca accggcccag cttgaggatg acgaagtggt tgccgaccat gtaggcgtgg
     7741 tgcgggttgt gggtgatgaa gatgacgccg agaccgctgt cgcgcatctt ggtgacgtac
     7801 ttcagcacga cgccggactg cttgacaccg agggccgccg tcggctcgtc gaggatgacg
     7861 acgcgggcac cgaaatagat cgcgcgggcg atggcgatgc actgccgctg gccgccggag
     7921 agggcgccga ccggacggtc gaggtccggt atgacgatcc ccatcttctg gagttcctcg
     7981 ccggagatgc gcttcatggc gtcgatgtcg aggccgaaac ccttcttcat ctcgttgccg
     8041 aggaagaagt tgcgccacac cgacatcagg ggtgcgaggg ccaggtcctg gtagaccgtg
     8101 gcgatgccct tggcctgcgc ctcgcgcggg gagttgaagc ggatctcctc cccgtccatt
     8161 cgcaccacac cttcggtgtg ctggtgcagc ccggcgatga tcttgatcag tgtggacttg
     8221 cccgcgccgt tgtcgccgag gacgcaggtg acctcacccg gactgacgct gatgttgacg
     8281 ccgcgcagtg cgtggacgtt gccatagctt ttgccgacct cgtcgagttc gagaagtcgg
     8341 gttcctgtcg ccgttgccgt ggtcatgcgt ccgcccgctt cctgacgtag ttgttgatga
     8401 ggacggccgc gatgagcatg acgccgagga aggtgcgcag ccagtcgaca tcccagccgg
     8461 catagctgat gcccagcacc gccatgccgt agatgagcgc accgatcgat gcaccgacga
     8521 tggagccata gccgccggtg agcagacacc cgccgatgac ggccgcgatg atgtagatga
     8581 actcgtagcc ggggccggtg ccggactgca gggtgttgta ctcgaagagg gtgtgcatgc
     8641 ccagcagcca ggctgcgaaa ccggtcccca tgaagagcgc gatcttcgtc gcgaccaccg
     8701 gaacacccac cgctcgagcg gcagcggagt cgccaccggc ggcgaggatc cagtttccgt
     8761 acttggtgcg cagcagcaag taggagacgg cgacggtcaa caggatccag tagatgatcg
     8821 ggatcttgat gttcacggaa ccgatcgaga actccgatgc gaacacggcg tgggcggaat
     8881 tgaaaccctc catgtcactg atcgagttcg aggacacgcc gccggtcacg atccgggtca
     8941 tagcgaggtt ggcgccccag aagacgaaat aggtgccaag tgtgacgagg aagctcggca
     9001 gcttcgtgac atgcagcagc cagccgttga aggcgccgat cgcgagggcg aagagcaacg
     9061 agatcaggac gccgacccag acgttgaggt tgaagtacca cgccgagtgg gctgccgtaa
     9121 gccctgcggt cgtcaccgcg acacctgtcg agaggtcgaa ctcgccaccg atcatcagca
     9181 gggccacgaa gacggccatg atcccgatgg tcgaggccga atagaggatg gtgccgatat
     9241 tgcccacgct ccggaaagcc ggtgcgaccg cgaggaagag ggcaaccaac accgcagcac
     9301 cgataagggc accgacagcc ggcatgccga agaaggtcga agcggcggac ctggtccgga
     9361 ctctgtcgtc cgaggcggtc gcggttgatg ctgtgctcat cggccgatca ccgggtcccg
     9421 ttggcagcga acttcgctac cgcttcgatg ttcgtggagt cgatgaacgc cgggccggtc
     9481 ggcacccgct ggccaccgcc gatcacgttg ccgttgatct tgtacagata gagcgacgtc
     9541 acggccaagt agccctggag gaagggctgc tggtcgatgg cccactggac cttgccaccc
     9601 ttgaccgcgt cgacgagtgc ggggttggtg tcgaaggtcc cgatcttcgc tgccgacccg
     9661 gcaccctcga cggacttgac agcgtccaga gcgaacggtg ccccgagagt gacgacccag
     9721 tcgatcgtct tgtcctgcgt cagctgcgcc tgcagcgacg tcaccaccgc ggcgtcgttg
     9781 cgaccatcga catagacccg cgcgaccttc acggcaccgc cggcaccgtc ctcgacgcct
     9841 ttgcagcgct cctccagctg ctgttggccc tgctcctgga tgacgcagat ggcgttcttg
     9901 gcgccgtcct tggccagtcg ctcgccgacc gccacgccag catcggtctc ggcctggccg
     9961 aagtagccga tcacaccgag gtccatggca tccgggccac cggcgttgaa catcgagacg
    10021 gcaatgccac cctcgatcgc cttcttgact acggggccga tggctccggt gttggggttg
    10081 gtcgcggcga tcccgtcgac tttcttgtcg atggccgcct ggatgagctg agcctgcttc
    10141 gtcgcgtcgg ggtcgttggt gtattcgaga ttcacaccga cgtcggtggc tgcctgctca
    10201 gcgcccttgc ggatgatgtc ccagaaggtg tcaccgggag cctggtgagt caccatcgtg
    10261 acggtcatgg tctttgcctt ggcgccgtcg gcaccgacct tctgctcgac ggcctgccgg
    10321 ccgccggtcg aggagcacgc caccagcgag cccatcagtg agagtgatac ggcggcgacg
    10381 ccgagcttct tgcgttggat catgtgagca ctgacctttc gaacatcacc gcttcgtcga
    10441 ggcggtgtcc ttgcggtggg tggtgatctg cagtcggcgt cggtgccgct ccagaagatg
    10501 tcgcgggcgt ggtcacctcc ctgctgtgaa ggggagacgg gggtcgggcg ggaggtccga
    10561 ccaggcgtcc cgcacccaga cgtgtgccgg gtccatgcag atgcgccacg cgcgttccgc
    10621 gccggggccg gccatgacgt tgaggtagta gaggtcggcc gtcggcgcag ccatcgcggg
    10681 gccgtgccag ccgtagggca ccaggacgac gtcgccggag cgcacctcgg cgaccacgtc
    10741 gatgggccgc tccggctgct cgttcgaccc ataaactcgt tggtagccaa cgggatcggt
    10801 gccacgcggg tcggtgggct gaagctcgaa gtaatagatc tcctcgagct gggtctcctc
    10861 gtcggtctcg gtgtcgtgct tgtgcggcgg ccaggagctc cagttgcccg ccggcgtgat
    10921 gacctcgcac acgaggatcg agtcggcgtc gaggacctcg ggcagtccga aggagcgcac
    10981 ctcccgcgaa gaggtgccgg caccacggat ctcgacgggc acctcgtcag cgcggatgtg
    11041 gcggaacggt ttcggggccg cgagcttcgt cgccggtgcc cccgtgaggg ccacgcgggc
    11101 gggaccaccc tcggcggtga cgcgcacgac ggcgccgcgg ccggcatacg caacatcggt
    11161 cgcggacgcg aagacgtgcg cccggccggc cagcacgatg tcgtgcgcgg tgccgtcttc
    11221 gtccgtgacc tcgacgcgga ccgagccgtt cagcggcacg acgatcgtct cttcgacgcc
    11281 gctgtcgtgg gtcaccgacg cgccctcgct gatctcggcg acgcgcagca tcgtatgctg
    11341 ccagccgtcg atacccgcgt cgatcacggt cgcgtaatct ccttgggcgg cagcgccttt
    11401 cgggaaaacc cagtgctcgt tgtcgcggcc ggtgccggtg gtcatggggc ctcctggtgg
    11461 acgagtcggg cggcgatgtc gacggcggcg gcgacgtcac cgtccggggg gaagagcagg
    11521 gcgcgaccga cgacaagacc ccgcacaggc gggatggtca gggcatgggc ccaccgctca
    11581 taggtctcgt cggcgcggcc ctgcgggtcg ccgccgagca gcagcgtcgg cagggtcgtt
    11641 gcctccatca cgcgctccag gtcgtcgacg accggcagct tcagccaggt gtgggcgctg
    11701 ctgtcgccga gcccggaggc gatgtggatg gagcggatcg tcgagtcggg gtccagcagg
    11761 ttgtgcaccc gcccgtcgcg aacctccgac atgaatggct cgatcatcgc catcagtccg
    11821 tatgccgcga gctcgccgat cgcccgagcg ctcgcttcga gggtgggcgc ggtgccggcg
    11881 ttgccgagcg cgatgcgggt gagcatcttg ccgccgtcga gaccccgctg ctgcaacgcc
    11941 gccgccgtgt aggccgtgaa gcggtcgtcg agttcgaagc aagccccttg cagcccaccg
    12001 cgattcatcg agccgatcgc gagcttgccc tccagggcgc ccatcagcac gaggtcgtcg
    12061 agcacatcgg gcgtgccgag cacaccgtcg accccagggc gcgacagggc actcacgagc
    12121 cgctcgagca gttcggcgcg gctggccatg gcgagcgggt cggtgcgcac gcccaaggcg
    12181 ccgcgggcgg ggtgatcggc ggccacgatc agcaaccggc cgtcggcacc cacccgttcg
    12241 cgacggcggc gcgtcgccca ggccgcggcg atgcgttcgg gttcgcggac gcggatctcg
    12301 gtgacgccgg ccatcgtcag cggggtggcg gcggcggtca tgattcggac ctccggccgt
    12361 ccagagccgc ctcgacctct gcggtcgtgg gcatggcggt cgagcactcc agtcgcgagg
    12421 cgacgatggc acccgccaca ttggcgaact cgacggtccg gcgcaggtcc caaccggaga
    12481 gcagtccgtg gatgaggcca ccgccgaacg cgtcacctgc accgagtccg ttgacgacct
    12541 cgacccagtg cggagccact tcgatgcgct cctcggcagt ggccgcaagc acacctttgg
    12601 gcccctgttt gacgatggcg aggtcgaggc cccgctcgag cagtgcatcg gcagcgcgct
    12661 gcggatcgcg ttctcccaca gcgacaaagc attcttcggc attgccgaca gccacgctca
    12721 cgtgctccag cgcacgcgcc acttgcgtgc tcgcctgtgc cgggtcgtcc cagaacatcg
    12781 gacgatagtc gaggtcgagg accgtatggc tgcgtcgtcc cctgcgctgc cacgccgcga
    12841 aatgcgttgc ccgagagggc tcttgggaca gacccgtcac agttgcccag aagaccggag
    12901 cactcgcgat ctcatccagc ggcagatctg cctcggtcag caacagatcc ggcgcggccg
    12961 gatagcgata gaagtagagc gggaaattgt cgggcggaaa gatctcgcag aaggtgaccg
    13021 gggtgggggg cccgtcgccg tcgatcgtcg agatgaaccg tgggtcgatg ccgagacgcg
    13081 cgctctccgc gtggacgaac cgcccgaagg ggtcgttgcc caccttggtc accagcgcaa
    13141 cgtgatgccc atgccgtgca gcggccacgg cgacattggt ggcgctgccg ccgaggaact
    13201 tgcggaaggt gctgacatcc tccagcccca cgccgtggtc gagcgggtag atgtctaccc
    13261 cgctgcgccc caacgagatg acgtcgaacg tctgaccgtc tgccatgacg tcaggttcca
    13321 tccgcaatgg gcgcgtcgag gccgggaatc tcactcatgt gcacgagccg agcctgggcg
    13381 cgggacaggt cgcaggcgtg ggcgatccgg ctggcttcga gagcatcccg aactgtgcac
    13441 gggctgggtg acgtggtggc gaccatgccg gcgaaggcgc tcagctcggc cagataggcg
    13501 tcgtggaagc gctcgaagaa ggaccagcac tgcgggccgg cggggaactg gacaccgtcc
    13561 tccagcgagc gcagtgccag cgagtcgtcg agtcccacgg cgacggcacc gcccgagccg
    13621 gtgacctcca tccgcacgtc gtgcccggca ccgttgtagc gcgtggccgt caccgtgacg
    13681 agggtgccgt cgtccagggt gatgatggcc gccgcggtgt cgacgtcgcc ctcctcggtg
    13741 aacatcgggt cgcccttgtt ggcgcccgtg gcatagacgg tggtcggctc agcgccgctg
    13801 acaaagcgga tgatgtcgaa gtcgtggata ttgcagtcgt ggaagatccc gccgctgacc
    13861 tttacgtatg cccgaggagg cggcgcctgg tcgtgggtcg tcgcgcggat ggcgtggatg
    13921 gtcccgatct ctccgctggc cactgcggcc cgcgcgcgcc gataggccgc atcgaagcgg
    13981 cgctggaagc cgatgtgcgc caaggtgccg gtcttggcct cgagttcggc aagctcgatg
    14041 gtctccccga gagtgcccgc gaccggcttc tcgcagaaga ccgggatccc cgccgtgaga
    14101 gcggcccgca gcaacggagc atgaccgtcg gttgccgtgg cgatgaccag gccgtcgatc
    14161 ccctgatgca cgaggtcgag agggtccgcg accacctcgg caccgatctc agcagcgacc
    14221 gacgaggcgg cctcgtgatt gagatcggcg accacgattg tgtcgacgcc agcaacatcc
    14281 ttgagggtgc gtgcatgaaa ggcgccgatg cgcccgactc cggcaaccgc gagcttcatg
    14341 ccaccgtcct atcgccatcg attgtccgtg tgctcctcgc ggccgtccgc gtgggcacga
    14401 ccacagcatc cccggcaggc tcagccatgt caagaatttg tcaggacatt ctcatatgtt
    14461 gccatcgtca tctcgtggcg aggtgccagc ctcgcacttc tcgtggctaa agagtcaccc
    14521 agccggcacc agcagccgag ttgaccatgg catcgagggc gtgcgcggag gccaccgcat
    14581 cgccgagcac cggaccctcc ggctgaccgc tcaggatcga ccggacgaag ccggcgagct
    14641 cgatgacctt cgtgtcgtca tagctcgtgg gaatgctgga accgggctgg aagcggccgt
    14701 ggtccccggc accagggccg acgagcaggc gggtgctggg ctggtcggcg tagttggtgc
    14761 ccgtggacag gcgcagctca ccgggcgcgc ggaagtccca ctccaccagc ccttgtgacc
    14821 cgtgcacctc gatgccgtag ttgttctgct cgccgacggc gacccggctc gactccaggg
    14881 cgacgaggac accggaccgc atacgcatca tcgcctggac atagtcctcg ttctcgacct
    14941 gccccttggg tgcgtcggga tcgccgaggc catgcccgta ggtcatggca ccgggcacga
    15001 tcgtcggccg ctgcgcgatg aagatggcgg tctgggcgac gagcgactcg atgtcgccca
    15061 gcacgtagcg cgtcaggtcc acggcgtgcg aggccaagtc gccgaggact ccatgaccac
    15121 ccgaggccag ggagtagcgc caggtcaacg tgccgagtgg gtgcgcggca tagtcggtga
    15181 gcagccggac gcgggcgtgc gtcggagcgc cgatcgcgcc ctcggccacg aggtcgcgca
    15241 gcttcgccat ggcggggatg gcgcggtagt tgaaccccac cgtgccctgc actccagcgt
    15301 cggcgacggc gtcggcgacg gcttgggcgt cggccgcgga gaggccgacg ggcttctcga
    15361 tccacaggtg cttgccggcc tgcgcgacgg cgacaccgat ctcctgatgg agggcgttgg
    15421 gggcggtgac gctgacggcc tcgactgccg ggtcggcgag caggtcgcgc cagtcgtcgt
    15481 atgccgtggc gaacccgtgt ttgctcactg ccgcttcgcg tgcggcaggc acgctgtcgg
    15541 caacggccac gaagaccggt gtcaccccga ggtcggcgta gtggtggcgc agccgagccc
    15601 aggcgcgcgc gtggacgtcg ccgagccagc cggccccgat gacaccgacg ccgatcgtgc
    15661 gtcgtccagt cgaatccatt tgtgccatgg ctgttccgtt ctgctcaagc gggagtcagg
    15721 ggttggggga ggccggtctc ggcctcgcgg cggagcgccc gcagctggtc tcgtatgccg
    15781 tagggcttga ccccgagcac ctcgtcgctg cgtggggtcg ccaagctggt gtcggccggc
    15841 atgggcgcgg gcagcatctg cccctcgggc acggggctcg tcgtgatcag ggatgcgtcg
    15901 aggtcgaaga cctcggccgc agcctgcgcc agctcgagcc gggtcaccgc gtccgcgccc
    15961 accacgtgca ggatgccgtc ggcctcgcgg tcgagggcga gcaacagccg cgcgccgatc
    16021 tcggcagcgg tcacggggga ggcgacattg ttgatcgccg ggtcctgcca gaccgagaac
    16081 tgctgaccgg ccgacaggga atcgaccagg gagagaacga aatagccgaa accgcaatct
    16141 tgcgcacgcg gtgccgaggg ctgcgtgagg tggcggccct gcacgccacc gacgcgcgcg
    16201 acgaacccgt gatctgcccg gtcgatcacg acctgctcgg acaatgcctt gtagaggccg
    16261 tagaggttga gcggattgac gggctcgtcc tcgtcgacat cgtggccgaa gccgtcgaac
    16321 acccagtcgg tggagaggta gccgaaccga gcacctgccg cgttgcacgc atccacgagg
    16381 cgacgggtca tcccgacgta ggcgtcgaac ccggccttgc ggtcccggta aaggccctgc
    16441 agatcgttgt cgatcgccag gtgcaacacc gcagtgctct cgtggtcggc aagggcagcc
    16501 gcgatgtcgc cggggtcggc gaggtcggtc agtcgccagg cccacgggaa gtccgggccc
    16561 ggatcgcgcc ggacggcaca gagcacgtcc gccccgcggc cggcgagcac ggaagcgaca
    16621 ttgccgccga cgaaacccgt tccgccggtg agcaataccc ggtggctcat gccgggtccg
    16681 acgagatctc gtcgagacga acgggacgcc cctctttgag cgaccgggtc gcggcgatgc
    16741 cgatgcgcac cggctcgcgg ccgtcggtgg tgcccacagg agaggcgcca ccgtcacgga
    16801 catagtcggc gaaggcggcc cactcgaggc ggtaggacgg cagataccgg tcgaggaaga
    16861 accacggcag cggctccgcg gccgtcgtgt ccggggtgcg cacccacgcg gcgtgtcgaa
    16921 gggggttgtc cgagagcgcc ataccgccgg agccgaaggc ctcgacccgc tggtcgtagc
    16981 cgtagacggc ttcgcggctg ttgtcgatcg tggtgatcgt gccgtcctcg tgggtcagca
    17041 ccacgacagc ggtgtccaca tcaccggcgt caccgatgcg cgggtcgacc cgcacggcgc
    17101 cgcgggcgta gacctcgacg acctcggagc cgacgacata gcgggccatg tcgaagtcgt
    17161 ggatcatcat gtcgaggaag atgccgccgc tgacggcgac atactcgggc ggcgggggcg
    17221 cggggtcgcg gctggtgatg cgcaccaggt gcagctcgcc gatctggccc gtgcgcacgg
    17281 cggcggcgac cgccgcgtgg cccgggtcga agcggcggtt gaagccgacc atgaagggaa
    17341 cgccggcggc ctcgacggcg gcgagggcgc ggtcgacctc ggcgaggtcg aggctgaccg
    17401 gtttctcgca gaggattgcc ttgccggcct cggctgcctt gatcgtcagg tcgacgtggg
    17461 tgtccgtgct cgtgcagatg gcgacagcgt ccacctccgg gctggccagc agctcgtcga
    17521 tgccgagggc cgccaccgcg aggtcgccgg ccacctgttg agcgacctcg ggcacggcgt
    17581 cggcgacggc cgtgaccgtc gtgccgggca cttcacgaag cagcaacccc gcgtgcatcc
    17641 ggccgatccg gccagtgccc acgacaccca tccgcacggg tgggcggctg tccgcctcgg
    17701 caaccccaaa ccctgcaccc tgcccggcca tgccactccc atcgttggtt ggtgatgcgc
    17761 gatcggcgag ctgagcgctg gggcgcgccg acgcgcgggt cgacaccaca tactcaagag
    17821 cctccgtctt caaatgtcaa gacattaggc caataagccg gtgacgtatc cggtgctgtc
    17881 aggacaccat gtcatgagcg gctctttgtc gctttgttcg tatgtccggt gacctacact
    17941 gcgccgatgg acaccatccc ggtcgtcatc gatcgcgcca gcccggtccc gctctatcac
    18001 cagctggccg agcaactcac cgcggcgatc gagagcggcc gcctcggccc gggcgacccg
    18061 ttcgagaacg agttggcgtt ggccgatcgg ctggacctgt cacggccgac cgtgcgccgg
    18121 gcgatcgccg aactggtcca gcgcggactg ttggttcggc ggcggggtgt cggcacgacg
    18181 gtcgccagtc ccatggtgca ccgtcgcgat gagctgacga gcctgtatga cgacatggag
    18241 cgccgcggtc agcatccggt cacgcgggtg ctgcgtctgg agcacgacgt gcgcgacccc
    18301 gtggccgcgg aggcgctcgg cgtgccggcc acgacgccat tggtgttcat cgagcggctg
    18361 cgcagcgccg gcgacgtccc cgccgcgctg atgcacaact ggctcccgcc ggcatacgcc
    18421 gacatcacct tcgccgacct cgagggcggc agcctctatg gctggctcaa ggcgaggggc
    18481 atcaaacctg ttgtggcaca ccaggttatc ggggctcgtc ggccgacggc cggcgagcgc
    18541 aagctgctgc gactcgcggc cggggacccg ctgctgacca tgtcgcgccg ggcgtatgcc
    18601 gcggatggct cggccgtcga gtacggcgat cactgctacc ggttcgacca gtacgccttc
    18661 gacgtgaccg tccacgagag ataacacgcc gtacgaagaa tccccacgaa gaaaccccac
    18721 gaagtgtcct cgctcctgcg tcgctgcggt acttcgcgag gaccccgagg agaccccacg
    18781 aagaaacccc acgaaagtgc cctcgctcct tcgtcgctgc ggtacttcgc gagatgtgct
    18841 cgctcctagt tcggcgcctt gacgaccagg acgggaacgg gagagtcgag gatcagggtc
    18901 tgggtgacac tgccgagcag cgccttgccc atgggggaac ggcggcgtgc accgatcacg
    18961 agcagcgtgg ccccagttcg cttgacggca tcgaggatcg tctccgcagt gtcgtcgatc
    19021 tcggccgagg ccgcttcgag gtcgaggctc caagaaacgt ccgcgaccga cgccgcggcg
    19081 aaggcctttg cgaggtcgtc gccgaccgag gcttcgagtg cggcccggtg atcctcgtcg
    19141 gccgccttct cgacgctgat cacgatcagc tcttcgccgc gctgggcggc ctggcgcgct
    19201 gcttcggtga ggacggcctg gctgtagcgc gaggtctggt gggcgacggc gacggtcata
    19261 cggtctcctg atggctgagg tgtgggtgag cggcgggtcg aggtgacgtc gtcaggctaa
    19321 gcctcgatcc accgatcgac ttggcgcggt gtggtgatcc ggcgcgtcgg aaca
//
LOCUS       DAOPJG010000040        19341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_2991_length_19341_cov_7.293736, whole genome shotgun sequence.
ACCESSION   DAOPJG010000040 DAOPJG010000000
VERSION     DAOPJG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 19341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19341
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_19341_cov_7.293736"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2798
                     /locus_tag="PKU75_05625"
     CDS             <1..2798
                     /locus_tag="PKU75_05625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HPF80393.1"
                     /translation="MRALDSGRINHAYLFSGPRGCGKTTSARILARCLNCEQGPTGTP
                     CGECDSCVALARGGPGSIDVIEIDAASHGGVDDARELRERAAFAPAESRYKVYIIDEA
                     HMVTTQGFNALLKIVEEPPEHVKFVFATTEPEKVIGTIRSRTHHYPFRLVPPARLHDY
                     LELLCRAEGVDVEPGVLSFVVRAGGGSVRDSLSVLDQLMAGSDARGLTYDGAAALLGF
                     TDGELLDATVTAFGAGDAAGVFTQIDKVIETGHDPRRFIEDLLERFRDLIVLTAVPDA
                     GSSVLRALPSDQIERMRVQAGGFGPGALSHAADLVNTGLSEMTGATAPRLQLELIAAR
                     VLLPGAAGEHGYAARLDRIERRLDVVGAPSPRATPPLGEAQPPRATQPLGEAQPPGEA
                     QPPQQPGEIQPRGEAQPRGEAQPQQPGPNPPAAPQPDGGARQSDPPPPGPAASQPGTA
                     PLTQAANAAAPSVSGPSGGRLDAGPDGEQPQQARGGGQPQQRPAGPRPRIRMGSPEPS
                     APAPGPDAAASAGGGRGPGGLDLEALRRSWPDVLARIFTLKRTTWTFLSEHAQVDSYD
                     GQRLVLSIGTVGLANTFRSGIHPEIVRQALIDVLGLDARVDAIARTGGAGGPGGGDGP
                     GDGRRDGRGGGPGTSNGGARNGSDVPGPQGDSSRQWQSTRDEQPVASGSPGPGAPAGS
                     NGPDRAAESSHREPGGPERASVAGGRRTQPGGPPDPGADPAMRRDWSEGGPAWGSGPA
                     SGAPSWASGGAGPAASTSDSAVPDSAALASAASVSVAPGSAAPDSAAPYSAASAHSAA
                     GAAAPTERMSPQSSPGRSSPTTASPEPEPAGAAANASANASADPSAWTPTTSYPTTSY
                     PSTSYPSTAYPSTADPATVAPATTDPATMDPTAGLSDEDLVSADDEDLMDLGEVGQPV
                     VERVLGGTVIWREDESS"
     gene            complement(2968..3339)
                     /locus_tag="PKU75_05630"
     CDS             complement(2968..3339)
                     /locus_tag="PKU75_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80394.1"
                     /translation="MPNSRGMPRSRLLPLLTVLGLAGVTGCGDPPPAADVAPVEVVLP
                     GCTLNRDSVAVGTHEFSAAGTGYVVVTGADSTVLAEFAAPTTTPASVTLTASGPLTVT
                     CRAAKDADSGATATLTVNAGK"
     gene            3406..4005
                     /gene="recR"
                     /locus_tag="PKU75_05635"
     CDS             3406..4005
                     /gene="recR"
                     /locus_tag="PKU75_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPF80395.1"
                     /translation="MYEGVVQDLIDELGQLPGVGPKGAQRIAFHLLQADPADVQRLIT
                     ALSEVKDKVTFCTLCGNVAEAALCRICQDPRRDGHAVCVVEEPKDVVAIERTREFRGR
                     YHVLGGAISPMDGVGPEQLRIAELMARLGDGEITEVILATDPNIEGEATASYLARQLK
                     TFEIRVTRLASGLPVGGDLEYADEVTLGRAFEGRRLIDA"
     gene            4016..4594
                     /locus_tag="PKU75_05640"
     CDS             4016..4594
                     /locus_tag="PKU75_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HPF80396.1"
                     /translation="MQPPPSVGGDADLAELAGLTASEARHFIAAITEIASGSAPDAAL
                     PLALLATADILTTGARLGAIQDIVLDERYEPDDGDDTDLAALRAALANVFEGLDEYAD
                     LVDPVTSTELTTGSLATDFSVIVGALDHGLKHFARGNQVEALWWWQFSYLSDWGERAA
                     MALRVLHGLLAHVRLDADDDLVAEAEFDALHP"
     gene            4695..5966
                     /locus_tag="PKU75_05645"
     CDS             4695..5966
                     /locus_tag="PKU75_05645"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPF80397.1"
                     /translation="MALVVQKYGGSSVADAESIKRVARRIIETRKAGNDVVVAVSAMG
                     DTTDELLDLANEVSPMPPPREMDMLLTAGERISMALLAMAIADQGHSVRSFTGSQAGV
                     ITDTTHGKAKIIDVTPGRITEAIGKGHVVIVAGFQGVSRETKEITTLGRGGSDTTAVA
                     LAAAMNASVCEIYTDVDGVFSADPRVVPKARKIARITSEEMLELAASGSKVLHLRSVE
                     YARRFGIPIHVRSSFSHKEGTFVTDQPAPEGEPVEAPIIAGVAHDRSEAKITVVGVPD
                     RAGMAATIFAAIADADINIDMIVQNVSATETGLTDISFTLPKGEVTAAMQALQRIQGE
                     VGFVSLLHDDLIGKLSLVGAGMKSSPGVSATFFKALADADINVEMISTSEIRISVITR
                     EDRLDEAVRAVHTAFALDTDQEAVVYAGTGR"
     gene            6121..6978
                     /locus_tag="PKU75_05650"
     CDS             6121..6978
                     /locus_tag="PKU75_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPF80398.1"
                     /translation="MPTAPEYARRSYRGRQRRRLALLVTLPSLILGTVSVGGAYSAGL
                     LTRKPAAVCKPTVVEAPARDSFKIEVQNSNETPGEGAKVAKDLTKRGFTVTQVTNAPD
                     GVYIKRSALVYHGKQGLDQALLVAAQIPGARAWNDGRAGTGVELVIGYGFNGLSYVPP
                     APPPLPGEITVNVYNTTYREGLASEVGDALRDRSFELGSVGNDPLMSFLPDDVAVIRH
                     GPEGADAADVLLQHIPGARLVTDTREDTSLDLVLGNDYAALTPEAEIPKPAPRPEPKP
                     DTIERPCTK"
     gene            7090..8292
                     /locus_tag="PKU75_05655"
     CDS             7090..8292
                     /locus_tag="PKU75_05655"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPF80399.1"
                     /translation="MPVRKVAMLTAGGIAPCLSSAVGGLIERYTELAPEVEIIGYLDG
                     YAGLLTGNSITVTPEVRAGAHLLHGFGGSPLGNSRVKLTNVKDCVKRGLVQEGEDPLK
                     VAADQLTKDGVDVLHTIGGDDTNTTAADLAAYLHENGYELQVVGMPKTIDNDVVPIKQ
                     TLGAWTAAEQGAIYAENIIGEHTSNPRMLIIHEVMGRHCGWLTAETARRYRERMSGRE
                     YLPGVVAASKEAWDVHAVYVPESHIDIKAEGERLRHVMDELGCVNIFLSEGAGVEDVI
                     AELETAGEDVPRDAFGHVQLDKVNPGAWFAKQFAEAIGAEKTMVQKSGYFSRSAAAND
                     EDLALIKRCTDFAVDAALRGESGVVGEDEEQGDELRAIEFPRIRGAKPFDPSVDWFQA
                     LLKSIGQA"
     gene            8359..8742
                     /locus_tag="PKU75_05660"
     CDS             8359..8742
                     /locus_tag="PKU75_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPF80400.1"
                     /translation="MERRHASSGSPYEASIGFSRAVRVGNHVAVSGTAPIWPDGGIVL
                     DPHAQAKRAFEIALEALAELGGAVTDVVRTRMFITRTDVATEVSEAHGETFGDVRPAA
                     TMVVVSALLDPRWLVEVEVDAILND"
     gene            8777..9793
                     /gene="fgd"
                     /locus_tag="PKU75_05665"
     CDS             8777..9793
                     /gene="fgd"
                     /locus_tag="PKU75_05665"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HPF80401.1"
                     /translation="MTLKIGYKASAEQFDPARLAAFAVLAEEVGLDSVTVSDHYQPWR
                     LQGGHAPNALVWLSYVAARTERVTLGTSVLTPTFRYNPAVVAQEFASLACLAPGRVFL
                     GVGTGEALNEIAVGAVGEWPEFKERFARLREAVTVMRRLWTEEEVTFEGDYYRTEGAR
                     IFDRPEHPVPVFIAAGGPMVARYAGRHGEGFICTSGKGRELYADQLIPAVAEGRAKAG
                     RAGDPIERMIEIKISWDPDPELALQNTRFWAPLSLTAEQKHSITSPAEMEKAADELPI
                     EQVAKRWIVASDPSAAVEAVKFYTDLGFDQLVLHGPGEDQERFLRTLAEQVLPGLREL
                     QPAT"
     gene            complement(9803..11548)
                     /locus_tag="PKU75_05670"
     CDS             complement(9803..11548)
                     /locus_tag="PKU75_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HPF80402.1"
                     /translation="MHCIGIDLAWGDRAHTGVAVLDDGGRLELVTGATDDASIARALA
                     PYAERDVVVAIDAPIVVTNATGARAAERALGADFARFDAGAHPTNTGRPEFAEGSRAS
                     RLVAALDLTVTLDPQTSRRAIEVYPHAASIALFRLGRTLKYKNKPGRSLVAMRGELLR
                     LTQLLESLADATPRLDLHTAAWSDLVAGIAGATRKADLQRAEDQVDAVLCAYVARLAA
                     LDPQSLATYGDDASGVVVTPRLPADHQPEPRPARQPGRDRIVDEAVRIFREGHPRLER
                     AAAAYVDHVRGILDEAGINYLSVTGRAKSLKSFSEKAARSRDGRPLHPEPLADIHDQI
                     GLRVITFLRRDVAAVAELLAGEFTVLDDRDLGLETAGEGRFGYASRHLQLAVDTPGIA
                     GDGPLTATEVQVQVRTVLQHAWAEYEHAIRYKGSIPLAHASELDRRFTLAAGLLELAD
                     EQFTAIHEQLQQDAELVEEADDTALSGRDLAAYLAGHYRDAGWSREDHYVWMAALLGE
                     LGITRVPELAAVLAEVDEAELTSRLGYRYPAAAVRRLDDALLAHFGERYAALPGNAAR
                     RELLAARLARIGTAG"
     gene            11654..13165
                     /locus_tag="PKU75_05675"
     CDS             11654..13165
                     /locus_tag="PKU75_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPF80403.1"
                     /translation="MSSATGQVPMTAADHFLHRGEANPRTRSSVLSVELLEAPPDWDR
                     FLETFERASRSVARLRQRVVVPSAPTTSPRWVIDPDFDLSYHVRRVALPGNGSHRELL
                     DFAEQLFITPLDLQRPLWRVTLVEGLEGGGAAMISQLSHAITDGVGGVEMFAQVYDLT
                     REGRGLAMPELPVPHDLEPNDLALQGVKELPWRAVGVTRSALGLGLKAVGDPVGAVSG
                     ALGYAASLRRVLGAVAQAQPSPLLAGRGIQRRMLTLELDLPGLKAAGKALGGSVNDAY
                     LAAMCGALRRYHEGLGLPVDSLPMAIPVSLRATSDDAGGNRWTGLILAAPIGEADPAA
                     RIAAIRQIVATRRDEPALDVMGVVSSLTAMLPDLVVDPLVGAVAPVDVQCSNVMSYPG
                     PTFVAGAQVVAQYGMGPVPGVGMMATMITRLGRAFIGFRYDTASFVDDDLLEKCLREG
                     FAEVLAAGGVIAPLAGTHPVVKKKAARLAPSPKVPAARAASRTRAAATRKRAR"
     gene            13162..14622
                     /locus_tag="PKU75_05680"
     CDS             13162..14622
                     /locus_tag="PKU75_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HPF80404.1"
                     /translation="MNSAPRVPGTVAEVLGSPEGDHIAAFFDLDGTLVAGYTVTIYTK
                     AGLRNRDIGVLDFLRMVGLGLSYQAGAAPFESLVQQASSVFRGRTLDELHALGEKVFG
                     DQVADLLYPETRALVRAHRERGHRVVLCSSATSMQVQPVADYLGIDDVVCNQFVVDAD
                     GTLTGGLVEPVIWGEGKATAAQRYAASAGVDLADCYFYADGDEDVALMHLVGNPRPTN
                     PGPLMAKVAKKRGWPISRYSTRGIDGRESLVRNLIGASLLAPITAGAAVVGAITGSKR
                     SGLNVVTGLWPRALLEAQGVKLNVVGAHNLDAHRPAIFIFNHRTAMDTFIVSAVVRTD
                     FTGVAKHELKSNPLFGTFGRAMDIAFIDRENTDAAVSSMQDVERVLAKGISVVVSPEG
                     HRYDTHEVGAFKKGAFRMAMSAGVPVVPIVIGNADDVAARDSMTVNPGTVNIAVLQPI
                     SVSQWPEEELVERIDGVRDLYLDVLGNWPAEGDPRV"
     gene            14615..16996
                     /locus_tag="PKU75_05685"
     CDS             14615..16996
                     /locus_tag="PKU75_05685"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF002886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HPF80405.1"
                     /translation="MSERTVSECTASERTVAGRLVVFAVTRTAVEERLVRDRVRQLFG
                     DGPDAPEVLGVPRAKASPADIEGLRVRLAEFGDVELLPMGVTWLPSEHTDGERKVRLR
                     DLARGHNPYKPTPRQQGAIARKEPDRGRVTEGASASVATLRERYVESGKDPAADADGF
                     ARFVIRRARLALQRAEAQLLGPQFKSAKLVKDEILANKHFQAGLQRIRLELGPDATSE
                     EKVEEILEEMATGWGRLFIDVYPRVGRAVFQRGFDPTIDTDTTEISRLKQTLAERPVI
                     FLWSHRSNLDTPVLAASLHENGLPLPHMFAGINMSFGPMGAILRRSGVIFIRRSIGDP
                     LYKFLLKEFVGYLAEKRFNLSWSIEGTRSRTGKMLPPKLGLLSFVADAYIEGRSDDIA
                     LQPVSIAFDQLHEVGEYAEYARGGKKAAEGMKWLYGFIKAQGQGNFGKIYIRYPEPVS
                     MRSLMGPPNGEIAQDEARRALALQKMAFEVAWRINMAMPVTPTALVTATLLATQGAAL
                     TSRQVTVALSQVLDYLENLAAPLASSVPNLRAEAGVEQVLRALAASGIVTRVEGARDV
                     VWLIGPDKQLAATFYRNSIIQFFQLGAICEIALALAARSDSGTKRLERFWRAVDRLRD
                     LLKFDFYFQEKSVYREQIAAQMAMADPGWEAKLTAADFDIRTMLRGRMPLVAPVSLRP
                     FFEAYGIVADVLAHHGAAGPIDKAQVTKDALAYGEQLIAQRRVASADPVSALLFATGL
                     QLADNQGVLVDGPDRSERAARFEQELREITEAVALIAAVSTEVFTEQMRAARA"
     gene            complement(17114..18337)
                     /locus_tag="PKU75_05690"
     CDS             complement(17114..18337)
                     /locus_tag="PKU75_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF80406.1"
                     /translation="MPLTPSRRSEVPPFTVMTVLQAVAEARAQGREVISLCAGEPGQG
                     APGPVRDVAAHLMINEIPLGYSETRGLLALRQAIAGHYLKWYDLDLDPERICVTTGSS
                     GAFLLAFLSAFDPGDRVAVARPGYPAYVNILRSLGCEVVELDTGEEVRFQPTAELLAA
                     AHTQAPLTGVILASPANPTGTMIADTELEAILTWCRDNEVRVVSDEIYHGVTFTGSVG
                     TCAWQLDENAVVISSFSKYWGMTGWRLGWMLMPADLAPRIDALAGNLALCAPVPAQHA
                     AVAGFTEESYAECERAVAGFALARKIVLDAIPRLGWAGVAPADGAFYVYAGIRDQLGP
                     YADSVAWCSALLAEQDVAVTPGTDFDGAHGHEYIRLSLARGPDAVAEAIRRILPFQHS
                     LANRGTSASVPRRKF"
     gene            complement(18358..18774)
                     /locus_tag="PKU75_05695"
     CDS             complement(18358..18774)
                     /locus_tag="PKU75_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80407.1"
                     /translation="MLLGDIVVGRILTTDRRDRLLTPLRPLLALPYLGFLFHPPVGVA
                     LVLGFVASAGYSASLVLQERLAHEADPDVEGQVFGLFGNGQMVGQQSLGALAGGLLAT
                     VLSPATSMGLLVAASVLVTVSLNAGLRRSRRSLANV"
     gene            complement(18738..>19341)
                     /locus_tag="PKU75_05700"
     CDS             complement(18738..>19341)
                     /locus_tag="PKU75_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80408.1"
                     /translation="DPVPHRLAGMRTYGELFAIGEFRALFVSRCLVIASLSLASLALA
                     TLAYEETGSPILSALALVGGSLLTLLGSLTALSLFDGLDARSAILLVIAVTGAQAALQ
                     AVPRLPLPMRFAVLLAGVRARPMPARPGNILAETHRVNAHVLGSPILRPIMLSAWIPM
                     GLIVGCEALFIPYGGKAWSGCLFAAAAAGCSSATSSSAAY"
BASE COUNT         3068 a         6746 c         6656 g         2871 t
ORIGIN      
        1 tgatgcgtgc gttggactcc ggccgcatca accacgccta cctcttctcc ggcccccgcg
       61 ggtgcggcaa gacgacgagc gcccgcatcc ttgcccgctg tctcaattgc gagcaggggc
      121 cgaccggcac cccgtgcggc gagtgcgact cgtgcgtggc gctggcgcgc ggggggcccg
      181 gctcgatcga cgtcatcgag atcgacgcgg ccagccacgg cggcgtcgac gatgcccgtg
      241 agctgcggga gcgggcggcc ttcgcgccgg ccgagagccg ctacaaggtc tacatcatcg
      301 acgaggccca catggtcacg acgcaaggct tcaacgctct gctcaagatc gtcgaggagc
      361 cgcccgagca cgtgaagttc gtcttcgcga ccaccgagcc cgaaaaggtc atcgggacca
      421 tccgctcccg cacccaccac taccccttcc gcctcgtgcc gccggcccgg ctgcacgact
      481 atctcgagct cttgtgccgc gccgagggtg tcgatgtcga gccgggcgtg ctgtccttcg
      541 tcgtgcgggc cggcggaggt tcggtgcgag attcgctgtc cgtgctcgat cagctgatgg
      601 cggggtcgga cgcgcgtggg ctgacctatg acggagcggc tgcgctcctc gggttcaccg
      661 acggtgaact gttggatgcg acggtcacgg ccttcggggc tggagacgcc gcgggggtct
      721 tcacccagat cgacaaggtc atcgagaccg gccacgaccc ccggcgcttc atcgaggacc
      781 tgctcgagcg cttccgcgac ctgatcgtcc tgaccgcagt gcccgacgca gggtcgtcgg
      841 tgctgcgggc gttgccgagc gatcagatcg agcggatgcg ggtgcaggcg ggcgggttcg
      901 gtcccggggc gctctcgcac gcggccgacc tggtcaacac cgggctgagc gagatgaccg
      961 gcgccacggc gccacggctg caactggaac tgatcgccgc ccgcgtcctg ctgcccggtg
     1021 ccgccggtga gcatgggtat gccgcccgcc tcgaccgcat cgaacgtcgc ctcgacgtgg
     1081 tcggcgcccc gtcgccgcgg gcgaccccgc cgcttgggga ggcccagcca ccgcgggcga
     1141 cccagccgct tggggaggcc cagccgccag gcgaggccca gccgccacag cagccaggcg
     1201 agatccagcc gagaggcgag gcccagccga gaggcgaggc ccagccacag cagcccggcc
     1261 cgaacccgcc tgctgccccg cagcccgacg gcggagcgcg gcagtctgat ccgccaccgc
     1321 ccgggccggc cgcatcgcag ccgggaacgg cgcctctcac gcaagctgcg aacgccgctg
     1381 cgccatccgt ctccggcccg tccggtggtc gtctcgatgc cggcccggat ggggagcagc
     1441 cccagcaggc cagagggggt gggcagcctc agcagcgacc ggccgggccg cggccccgga
     1501 tccggatggg gtcacccgag ccaagcgcac cggcacccgg accggatgcc gccgcatccg
     1561 cgggcggtgg ccgcgggccg ggagggctcg acctcgaggc gctgcgcaga tcgtggcccg
     1621 acgtgctcgc ccgcatcttc accctcaagc ggacgacatg gaccttcctg tccgagcacg
     1681 cccaggtcga cagttatgac gggcagcggc tggtcctgtc catcggcacg gtggggctcg
     1741 ccaacacctt ccgctccggc atccaccccg agatcgtccg gcaagcgctg atcgacgtcc
     1801 tcggcctcga cgcccgggtc gacgcgatcg cgcgcaccgg tggcgcggga ggtcccggcg
     1861 gtggcgacgg acccggtgat ggtcgtcgtg atggtcgtgg gggtggtcct ggcaccagca
     1921 acggtggtgc ccgcaacggc tccgatgtgc ctggcccgca aggagattcc agccgtcagt
     1981 ggcaatcgac acgcgatgaa cagccggtcg cctcgggttc gccggggccc ggcgcgccgg
     2041 ccgggagcaa cgggccggat cgcgccgccg agtcctctca ccgtgagcct ggcggacctg
     2101 agcgagcatc cgtcgcgggc ggccgacgga cccaacctgg tggaccgccc gatcccggtg
     2161 ctgaccccgc aatgcgccgt gactggtcgg agggcgggcc ggcgtggggg agcggaccgg
     2221 cctccggcgc ccccagctgg gcgtccggag gcgccggacc ggctgcgtcc acctccgact
     2281 cagctgtccc cgactcagct gccctggcct cagctgcctc cgtctcagtt gcgccagggt
     2341 cagccgcccc tgactcagct gctccgtact cagctgcttc cgcccactcg gccgccggcg
     2401 ccgccgcccc caccgagagg atgtccccgc agtcctcccc cggtcggagt tcgccgacca
     2461 cggccagccc cgagccggaa ccagccgggg ccgccgccaa cgcttccgcc aacgcctccg
     2521 cagatccgag cgcctggacc ccgaccactt cctacccgac cacttcctac ccgagcactt
     2581 cctacccgag cactgcctac ccgagcactg cggaccctgc cacggtggcc cccgccacga
     2641 cggaccctgc cacgatggac ccgaccgccg ggctcagtga tgaggacctg gtcagcgccg
     2701 acgacgagga cctgatggat ctcggcgagg tcggccagcc cgtcgtcgag cgcgtcctgg
     2761 gcggcaccgt gatctggcgc gaggacgaat cctcctgacg atgccgccgt ctcagccgtc
     2821 ggtgccatcg ctgtcgagcg caggcgcact cgattgccta ggccttcgac ggcgttaacc
     2881 atcacgagtt gttgtgatca ccaccattcc tgatgggtaa caccgtgtga ggacgggtaa
     2941 caccgtggga ggcacccggc ccccgcgtca cttgccggcg ttgaccgtca aggtagcggt
     3001 cgcgccggag tcggcgtcct tggcggcacg gcaggtcacc gtaagcggac cggaggccgt
     3061 gagcgtgacc gacgcgggcg tggtcgtggg ggcggcgaac tcggcgagca ctgtgctgtc
     3121 cgcgccggtc accacgacat accccgtccc ggcggcggag aactcgtggg tgccgacggc
     3181 cacggagtca cggttgaggg tgcagccggg caggaccacc tccaccgggg ccacgtcggc
     3241 ggcaggcggc gggtcgccgc acccggtcac gccggccaga cccagcacgg tgagcagcgg
     3301 gaggaggcga gagcgcggca taccccggct gttcggcata cccgactgta tgccgcccgc
     3361 cgcgccgccg ggcggttgtc ggccccaccg cctacccttg ccggtgtgta cgaaggtgtg
     3421 gtccaggacc tgatcgacga gctcgggcaa ttgcccgggg tcggccccaa gggtgcccag
     3481 cgcatcgcct tccacctgct ccaggccgat ccggccgacg tccagcgcct catcaccgcc
     3541 ctttcggagg tcaaggacaa ggtcaccttc tgcaccctgt gcggcaatgt cgccgaggct
     3601 gcgctgtgcc ggatctgcca ggacccgcgg cgtgacggac acgccgtgtg cgtggtcgag
     3661 gagcccaagg acgtcgtcgc gatcgaacgc acccgcgagt tcagggggcg ctatcacgtg
     3721 ctcggtggcg cgatcagccc gatggacggt gtcggccccg agcagctgcg gatcgcggaa
     3781 ctgatggcaa ggctcggcga cggcgagatc accgaggtca tcctcgccac cgaccccaat
     3841 atcgaggggg aggccaccgc gtcatacctc gctcggcagc tgaagacctt cgagatccgg
     3901 gtcacccgcc tcgcctccgg gttgccggtc ggcggcgacc tggagtatgc cgatgaagtc
     3961 accctgggcc gcgccttcga aggacggaga ctgatcgatg cctgacccga cgagcatgca
     4021 gccaccccca agcgtcggcg gcgacgccga cctggccgaa ctggccggcc tgaccgcgtc
     4081 ggaggcgcgc catttcatcg ccgccatcac cgagatcgcc tccggctcgg cgcccgacgc
     4141 cgcgctaccc ctcgctcttc tcgccaccgc ggacatcctc accaccggcg cccggctcgg
     4201 cgccatccag gacatcgtcc ttgacgagcg ctacgagccc gacgacggcg acgacaccga
     4261 tctggccgca ctccgggcgg cgctcgccaa cgtgttcgag ggcctggacg agtatgccga
     4321 cctcgtcgat ccggtgactt ccacggagtt gaccaccggc tccctcgcca ccgacttctc
     4381 ggtcatcgtc ggcgccctgg accatggcct gaagcacttc gcacgcggca accaggtcga
     4441 ggccctgtgg tggtggcagt tcagctacct ctccgactgg ggcgagcggg ccgcgatggc
     4501 gctacgcgtg ctgcacggcc tcttggcgca tgtgcgcctg gacgccgacg acgacctcgt
     4561 cgccgaagcc gagttcgacg ccctgcaccc ctagccacgc gcccgagtcg cgaacggcgc
     4621 gggcacggca taccgggcgt cgcctaccat cagggcgtcc tttcccgacc gaccgacaac
     4681 gggagtgcaa gccagtggcc ctggtcgtcc agaaatacgg cggcagctcc gtcgccgacg
     4741 ccgagagcat caagcgcgtc gcccggcgga tcatcgagac ccgcaaggcc ggcaatgacg
     4801 tcgtcgtcgc cgtatccgcc atgggggaca cgaccgacga gctgctcgac ctcgccaacg
     4861 aggtcagccc catgccgccg ccgcgcgaga tggacatgct gctgaccgcc ggtgagcgga
     4921 tctccatggc cctgctcgcc atggccatcg ccgaccaggg acacagcgtg cgctccttca
     4981 ccggcagcca ggcgggcgtc atcaccgaca ccacacacgg caaggcgaag atcatcgacg
     5041 tcacccccgg ccgcatcacc gaggcgatcg gcaagggcca cgtcgtcatc gtcgccggct
     5101 tccagggcgt ctcgcgcgag accaaggaga tcaccaccct cggccggggc ggctccgaca
     5161 ccacggcggt ggcgctcgcc gccgccatga acgccagcgt ctgcgagatc tacaccgacg
     5221 tcgacggcgt cttcagcgcc gacccccgcg tggtgcccaa ggcgcgcaag atcgcccgca
     5281 tcaccagcga ggagatgctc gaactcgcgg ccagcggcag caaggtgctg cacctgcgca
     5341 gcgtcgaata cgcccgccgc ttcggcatcc ccatccacgt ccgctcctcc ttctcacaca
     5401 aggagggcac ctttgtcacc gaccagcccg ctcccgaagg agaacccgtg gaagccccga
     5461 tcatcgccgg cgtcgcgcac gaccgcagcg aggccaagat caccgtcgtc ggcgtcccgg
     5521 accgggccgg catggccgcg accatcttcg ccgccatcgc cgatgccgac atcaatatcg
     5581 acatgatcgt ccagaacgtc tcggcgaccg agaccggcct gaccgacatc tccttcaccc
     5641 tgcccaaggg cgaggtcacc gccgcgatgc aggccctgca gcgcatccag ggcgaggtcg
     5701 gcttcgtctc gctcttgcac gacgacctca tcggcaaact gtcgctcgtc ggggccggga
     5761 tgaagtccag ccccggggtg tcggcgacct tcttcaaggc gctcgccgac gccgacatca
     5821 acgtcgagat gatctcgacc tccgagatcc ggatctcggt catcacccgc gaggatcgcc
     5881 tcgacgaggc cgtccgggcc gtgcacaccg ccttcgccct cgacactgac caggaagccg
     5941 tcgtctacgc cggcaccggg cgctgacagg cccactcctg gccgttcggt gccgtgaatg
     6001 ccggctattc accgactcgt tatcaaggcg accccgatcg tcatcgtgtt gctctagtgc
     6061 ttcgtgtgac tcatgaggca ccatggtgga cgccagctac tcgaccagat ggggatggtc
     6121 atgccgaccg cgcctgaata cgcccgccgc agctaccgcg gccggcagcg ccgtcgcctt
     6181 gccctgctcg tgaccctccc gagcctgatc ctcggcacgg tcagcgtcgg cggcgcctac
     6241 agcgccggcc tgctgacccg caaacccgcc gccgtctgca aacccacggt cgtcgaggcc
     6301 ccggctcgcg attccttcaa gatcgaggtc cagaactcca acgagacccc gggcgaaggc
     6361 gccaaggtcg ccaaggacct gacgaagcgc ggtttcacgg tgacccaggt caccaatgct
     6421 cccgacgggg tctatatcaa gcggtccgcc ctcgtctatc acggcaagca ggggctcgac
     6481 caggcgctcc tcgtggccgc ccagatcccg ggggcccgcg cctggaacga cggcagggcc
     6541 ggcaccggcg tcgaactcgt catcggctac ggcttcaacg gactcagcta cgtgccgccc
     6601 gccccgccgc cgctgcccgg tgagatcacc gtcaacgtct acaacacgac ataccgtgaa
     6661 ggcctggcga gcgaggtggg cgacgcgttg cgcgaccgca gcttcgaact cggctccgtc
     6721 ggcaacgacc cgctgatgtc gttcctgccc gacgacgtcg cggtgatccg gcacggaccc
     6781 gaaggcgccg acgcggcaga cgttctgctg caacacattc cgggtgctcg cctcgtgacg
     6841 gacacccgcg aggacacctc gctggacctg gtcctcggca acgactacgc cgcgctgacc
     6901 cccgaggcgg agatcccgaa gccggccccg cggcccgagc ccaagcccga cacgatcgag
     6961 cggccctgca ccaagtagct cctccgcgaa ggcccctgcc ggagtgaggt gggacgcgtt
     7021 tcccgacgcg ccgggagagt gcttggatgg gaggcagacg attcgtcgac tccaggaaag
     7081 ggtctcgtca tgccggttcg taaggttgcc atgctcactg ccggagggat cgccccgtgc
     7141 ttgtccagtg cggtcggggg actgatcgag cgctacaccg aactcgcccc ggaggtcgag
     7201 atcatcggtt atctcgacgg gtatgccggg ctgctcaccg ggaactcgat caccgtcacc
     7261 cccgaggtgc gcgccggggc gcacttgctg cacggcttcg gaggtagccc gctcggcaac
     7321 tcccgggtca agctgaccaa tgtcaaggac tgcgtcaagc gcgggctggt ccaagagggc
     7381 gaggatccgc tcaaggttgc cgccgaccag ctcaccaagg acggcgtcga tgtgttgcac
     7441 accatcggcg gtgacgacac gaacaccacg gcggccgacc tcgcggccta ccttcacgag
     7501 aacggctacg aactgcaggt cgtcggcatg cccaagacga tcgacaacga cgtcgtgccg
     7561 atcaagcaga ccctcggtgc ctggaccgcg gccgagcagg gggcgatcta cgccgagaac
     7621 atcatcggcg agcacacctc caacccgcgg atgctgatca tccacgaggt catgggtcgc
     7681 cactgcgggt ggctgaccgc cgagaccgcg cgccgctatc gggagcggat gtccgggcgc
     7741 gaatacctcc ccggcgtcgt cgcggcgtcc aaggaggcct gggatgtcca cgccgtctat
     7801 gtgcctgaga gtcacatcga catcaaggcc gagggggagc gactgcgcca cgtgatggac
     7861 gagctcggct gcgtcaacat cttcctgtcc gagggcgccg gcgtcgagga cgtcatcgcc
     7921 gaacttgaga ccgcgggcga ggatgtcccg cgcgacgcct tcggtcacgt ccaactcgac
     7981 aaggtcaacc ccggcgcgtg gttcgccaag cagttcgccg aggcgatcgg cgccgagaag
     8041 accatggtgc agaagtccgg ctacttctcc cgctcggctg cggccaacga cgaagacctc
     8101 gccttgatca agcgctgcac cgatttcgct gtggacgctg cgctgcgcgg cgagtccggc
     8161 gtggtgggcg aggacgagga gcagggcgac gagttgcgcg cgatcgagtt cccgcgcatc
     8221 cgtggcgcca agcccttcga cccttcggtc gactggttcc aggcgctcct gaagagcatc
     8281 ggccaggcct gaaatacgtt gccgggcaac gggatgcggc ataaaaccca cgcaggctca
     8341 aaggagagtt catccctgat ggaacgtcgt cacgcctcgt ccggctcgcc ctacgaggcc
     8401 tcgatcggct tcagccgcgc ggtgcgcgtc ggcaatcatg tcgccgtcag cggcaccgcg
     8461 ccgatctggc ctgacggggg catcgtgctc gacccgcacg cgcaggccaa gcgggcattc
     8521 gagatcgccc tcgaggcgct cgccgagctg ggcggcgcgg tgaccgatgt cgtgcgcacg
     8581 cgcatgttca tcacccgcac cgatgtcgca accgaagtct cggaagccca cggcgagacc
     8641 ttcggcgacg tccggccggc cgcgaccatg gtcgtggtct ccgccctcct cgacccccgc
     8701 tggctcgtcg aggtcgaggt cgacgccatc ctgaacgact gaacccgctg gccaacgcac
     8761 cgatagggtc accagggtga cgctcaagat cggatacaag gcatcggccg agcagttcga
     8821 ccccgcccgc ctcgcggcat tcgcagtgct cgcggaggag gtggggctgg actcggtgac
     8881 cgtttccgat cactaccagc cctggcggct gcagggcggt cacgccccca acgcgctcgt
     8941 ctggttgtcg tatgtcgcgg cccgcaccga gcgggtcacg ctcggcacca gcgtgctcac
     9001 cccgaccttc cgctacaatc ccgcggtggt cgcgcaggag ttcgcctcgc tcgcgtgcct
     9061 ggcgccgggg cgggtcttcc tcggtgtggg gacgggggag gcgctcaacg agatcgccgt
     9121 cggggcggtg ggggagtggc cggaattcaa ggagcggttc gcgcgcctgc gcgaagccgt
     9181 cacggtgatg cggcggctgt ggaccgagga agaggtcacc ttcgagggcg actactaccg
     9241 caccgaggga gcccggatct tcgaccgtcc cgagcacccg gttccggtgt tcatcgccgc
     9301 cggcggaccc atggtggcgc ggtatgccgg ccggcacggt gaggggttca tctgcacctc
     9361 gggcaagggc cgggagttgt atgccgatca gctgatcccc gccgtcgccg agggccgggc
     9421 caaggccggc cgggccggcg atccgatcga gcggatgatc gagatcaaga tctcctggga
     9481 tccggatccc gagctggcgc tgcagaacac ccgcttctgg gcgccgctgt cgctcacggc
     9541 ggagcagaag cactcgatca ccagcccggc cgagatggag aaggcggcgg acgagttgcc
     9601 gatcgagcag gtcgccaagc ggtggatcgt cgcctccgac ccctcggcgg ctgtcgaggc
     9661 cgtcaagttc tataccgacc tcggcttcga ccaactcgtc ctgcacgggc cgggcgagga
     9721 ccaggagcgc ttcttgagga cgctggccga gcaggtgctc cccggcctgc gcgagctgca
     9781 gccggcgacc taagggcccg gctcagccgg ccgtgccgat ccgcgcgagg cgagccgcga
     9841 gcaactcgcg gcgggcggcg ttgcccggga gggcggcata ccgctcgccg aagtgggcga
     9901 gcagggcatc gtcgagacgg cgcacggcgg cggcgggata gcggtagccg agccggctcg
     9961 tcagctcggc ctcgtcgacc tcggcgagga ccgcggccag ctcgggcacc cgggtgatgc
    10021 ccagttcgcc gagcagcgcg gccatccaga cgtagtggtc ctcgcgcgac cagccggcgt
    10081 cgcgatagtg ccccgcgaga taggcggcca ggtcgcggcc ggacagtgcg gtgtcgtccg
    10141 cctcttcgac cagctcggca tcctgttgca gttgctcgtg gatcgcggtg aactgctcgt
    10201 cggccagctc cagcagcccc gcagcgagcg tgaaccgtcg atccagctcg gaggcgtgtg
    10261 cgagcgggat cgaccccttg taacggatcg cgtgctcgta ttcggcccag gcgtgctgca
    10321 ggaccgtccg cacctggacc tgcacctcgg tcgcggtcag cggcccgtcg ccggcgatgc
    10381 ccggggtgtc gacggcgagc tgcaggtggc gactggcata cccgaagcga ccctcgccgg
    10441 cggtctccag ccccagatcg cggtcgtcga gcaccgtgaa ctcgccggcg agcagctcgg
    10501 cgaccgccgc gacatcgcga cgcaggaagg tgatcacccg cagcccgatc tggtcgtgga
    10561 tgtcggcgag cggctcgggg tgcagcggcc ggccgtctcg gctgcgggcc gccttctccg
    10621 agaacgattt caggctcttc gcgcggccgg tcaccgacag atagttgatg cctgcctcgt
    10681 cgaggatgcc ccgcacgtga tccacatagg ccgcggccgc acgttccagt cgcgggtgcc
    10741 cttcgcgaaa gatccgcact gcctcgtcga cgatgcggtc acggccgggt tggcgggccg
    10801 ggcggggctc gggctggtga tcggcgggca gccggggtgt gacgacgacc ccgctcgcgt
    10861 cgtcaccgta ggtcgccaga ctctgcggat cgagcgcggc cagccgagcg acatacgcgc
    10921 acagcaccgc gtccacctga tcctcggcgc gctgcaggtc ggccttgcgg gtcgcgccgg
    10981 cgatgccggc gaccaggtcg ctccaggcgg ccgtatgcag gtccagccgg ggggtcgcgt
    11041 ccgcgagcga ctcgagcagc tgggtcagcc gcagcagctc accccgcatc gcgacgagcg
    11101 agcggccggg cttgttcttg tatttgaggg tccggccgag gcggaacaga gcgatggaag
    11161 cggcgtgcgg ataaacctcg atcgcccggc ggctcgtctg cgggtccaag gtcaccgtca
    11221 ggtcgagggc agcgaccagg cgggaggccc gggaaccctc ggcgaactcc ggcctgccgg
    11281 tgttggtcgg atgggcgccg gcgtcgaagc gcgcgaagtc cgcccccagc gcgcgctcgg
    11341 ccgcgcgggc tccggtcgca ttggtgacca cgatcggagc atcgatggcc acgacgacgt
    11401 cgcgctcggc atacggggcc agggcccgcg cgatcgaggc gtcgtcggtg gcgccggtga
    11461 ccagctcgag gcgcccgccg tcgtcgagca cggcgacgcc cgtgtgagcg cggtcgcccc
    11521 acgcgaggtc gattccgatg cagtgcacct cgacaccctg tcacgccacg gggatcctgg
    11581 ccacgtcacg gacccgtgct cacgtggcgg cggtgagttg accaggtgtc ccggcgcccg
    11641 tgatccttgg cgcatgagtt cagcgaccgg ccaagtgccg atgacggcgg ccgatcactt
    11701 cttgcaccgc ggtgaggcca atccgcgcac ccgcagcagc gtcctgagcg tcgaactgct
    11761 ggaggccccg ccggactggg atcgattcct ggagaccttc gagcgggcgt cgcgctcggt
    11821 tgcgcggctg cgtcagcggg tggtggtgcc cagcgcgccg acgacgtcac cgcgttgggt
    11881 gatcgatccc gacttcgacc tgtcctatca cgtgcgccga gtggcgttgc ccggcaacgg
    11941 atcccaccgg gagttgctgg acttcgccga gcagctgttc atcaccccgc tggatctgca
    12001 gcggcccttg tggcgggtca cgctggtcga ggggctcgag ggtggcggcg ccgccatgat
    12061 ctcgcagctc agtcacgcca tcaccgacgg cgtcggtggc gtcgagatgt tcgcgcaggt
    12121 ctacgacctg acgcgcgaag gccgcgggct ggcgatgccc gagctgcccg tgccgcacga
    12181 tctggagccg aacgatctgg cgctgcaagg cgtcaaggaa ctgccgtggc gggccgtggg
    12241 cgtcacgcgt tcggcgctgg ggctgggact gaaggccgtg ggcgatcccg tgggcgctgt
    12301 ttccggcgcc ttggggtatg ccgcgtcgct gcgccgcgtg ctgggcgcgg tcgcccaggc
    12361 ccaaccgtcg ccgctgcttg cgggccgcgg gatccagcgt cgcatgctga ccctggaact
    12421 cgacctgccg gggttgaagg ctgccggaaa ggcgttgggc ggcagtgtca atgacgccta
    12481 tctcgccgcg atgtgcggcg ccttgcggcg ctatcacgag gggctcgggc tgccggtcga
    12541 ctcgctgccg atggcgatcc cggtgagcct gcgcgcaacc tcggacgacg cgggaggcaa
    12601 ccggtggacc ggcctgatcc tggcggcgcc gatcggcgag gccgatcccg ccgcgcggat
    12661 cgccgcgatc cggcagatcg tggcgacgcg ccgcgacgag ccggcgctgg acgtgatggg
    12721 tgtcgtgtcc tcgctgacgg cgatgctgcc ggacctcgtg gtggacccgc tggtcggcgc
    12781 ggtggcgccg gtggacgtcc agtgcagcaa cgtcatgtcc tatcccgggc cgaccttcgt
    12841 ggccggggcc caggtcgtcg cgcaatacgg catgggcccg gtgccgggcg tcgggatgat
    12901 ggccacgatg atcactcgcc tggggcgtgc cttcatcggt ttccgttatg acacagcgag
    12961 tttcgtcgac gatgacctgc tcgagaagtg cttgcgtgag gggttcgccg aggtgttggc
    13021 cgccggcggt gtcatcgcgc cattggcagg aacacatccc gtcgtgaaga agaaggccgc
    13081 gcggctcgcc ccgtcgccga aggttcccgc cgcgcgggcc gcgtcgcgga cgcgagcggc
    13141 ggcaacgcgt aagcgggccc ggtgaactcc gccccccggg tccccggcac cgtcgccgag
    13201 gtcctcggtt cacccgaggg tgatcacatc gcggccttct tcgacctcga cggcacactc
    13261 gtcgccgggt acacggtcac gatctatacc aaggccgggc tgcgcaaccg ggacatcggc
    13321 gttctcgact tcctgcgcat ggtcggcctg gggctgagtt atcaggcggg cgcggcaccg
    13381 ttcgagagcc tggtgcaaca ggcgtcgtcc gtctttcgcg ggcgcacgct ggatgagctg
    13441 catgcgttgg gggagaaggt cttcggtgac caggtcgccg atctgctcta tcccgagacc
    13501 cgggcgctgg tgcgggcgca ccgcgagcgg ggacatcgcg tcgtgctgtg ttcgtcggcg
    13561 acgagcatgc aggtgcaacc ggtcgccgac tatctcggca tcgacgacgt ggtgtgtaat
    13621 cagttcgtgg tggacgccga cggcaccttg accggtggtc tggtggagcc ggtgatctgg
    13681 ggagagggca aggccacagc ggcccagcgg tatgccgcgt ctgccggtgt cgatctcgcc
    13741 gactgttact tctatgccga cggggacgag gatgtcgcgc tgatgcacct ggtcggcaat
    13801 ccgcgcccga ccaaccccgg accgctgatg gccaaggtgg ccaagaaacg cggctggccg
    13861 atctcgcgct attcgacgcg cggtatcgat ggccgggagt cgttggtccg caacctgatc
    13921 ggggcgtcac tgctggcccc gatcaccgcc ggcgcggccg tggtcggagc gatcaccggc
    13981 agcaaacgct caggtctcaa tgtcgtcacg ggcctgtggc cccgggcgct gctcgaagca
    14041 caaggcgtga agctgaacgt cgtcggggcg cacaacctcg atgcacaccg gccggcgatc
    14101 ttcatcttca accaccgcac cgcgatggac acgttcatcg tgtcggcggt ggtgcgcact
    14161 gatttcacgg gcgtcgccaa acacgaactg aagtccaacc cgctcttcgg caccttcggc
    14221 cgggccatgg acatcgcctt catcgaccgg gagaacaccg acgcggcggt gtcctccatg
    14281 caggacgtcg aacgggttct cgcgaagggc atctccgtcg tcgtgtcacc tgagggtcac
    14341 cgctacgaca cgcatgaggt cggcgccttc aagaagggcg ccttccggat ggccatgtct
    14401 gccggtgtgc ccgtcgtgcc gatcgtgatc ggcaatgccg acgacgttgc ggctcgcgac
    14461 tcgatgaccg tgaacccggg caccgtgaat atagccgtgc tgcaaccaat ttcagtatcc
    14521 cagtggcccg aggaggaact cgtcgaacgg attgacgggg tgcgtgatct gtatctggac
    14581 gtgctgggca actggcctgc cgaaggggat cctcgtgtct gaacgcaccg tctctgaatg
    14641 caccgcttct gaacgcaccg tcgctggccg ccttgtcgtc ttcgcggtca cgcgcaccgc
    14701 cgtcgaggaa cggctggtcc gggaccgcgt ccgccagctc ttcggtgacg gtccggacgc
    14761 gccggaagtc ctcggcgtgc cccgggccaa ggcgtcaccg gccgatatcg agggcctgcg
    14821 ggtgcggttg gcggagttcg gggacgtcga actgctgccg atgggggtga cctggctgcc
    14881 gtccgagcac acggacggcg agcgtaaggt gcggctgcgc gacctggcgc gcggacacaa
    14941 tccctacaag ccgacaccgc gccagcaagg cgcgatcgcg cgcaaggagc ccgatcgagg
    15001 ccgcgtgacc gagggggcga gcgccagcgt ggcgaccctc cgggagcgct acgtcgagtc
    15061 cgggaaggat cccgccgcag atgccgacgg cttcgcgaga ttcgtcattc gccgggcccg
    15121 actggcccta cagcgcgcgg aggcccaatt gctggggcca cagttcaagt cggccaaact
    15181 ggtcaaggac gagatcctcg ccaacaagca cttccaggcc gggctgcaac ggattcggct
    15241 cgaactcggc cccgacgcaa cgagtgagga gaaggtcgag gagatcctcg aagagatggc
    15301 gaccgggtgg ggccggctgt tcatcgacgt ctaccctcgc gtgggccgcg cggtgttcca
    15361 gcgcggcttc gaccccacga tcgacaccga caccaccgag atctcgcggc tcaagcagac
    15421 actggccgag cgccccgtta tcttcttgtg gtcgcaccgc tccaatctgg acaccccggt
    15481 gctggcggca tcgctccacg agaacggtct cccgctgccg cacatgttcg ccggcatcaa
    15541 tatgtcgttc ggcccgatgg gcgcgatcct gcggcgtagc ggtgtcatct tcatccgacg
    15601 ctctatcggc gaccctctct acaagttcct tctcaaagag ttcgtcggct atctcgccga
    15661 gaagcggttc aacctctcgt ggtcgatcga gggcacgcgc tctcgcacgg gcaagatgct
    15721 tcctcccaag ctcgggttgt tgtccttcgt cgccgatgcc tacatcgagg gccggtcgga
    15781 cgacattgcc ctgcaaccgg tctcgatcgc gttcgaccag ttgcacgaag tcggcgagta
    15841 tgccgaatac gcgcgcggcg gcaagaaggc cgccgagggg atgaagtggc tctacggctt
    15901 catcaaggca caggggcagg gcaacttcgg caagatctac atccgctatc cggaaccggt
    15961 ctcgatgcga tcgctcatgg ggccgcccaa tggcgagatc gcccaggacg aggccaggcg
    16021 tgcgctcgcc ctgcagaaga tggccttcga ggtggcctgg cggatcaaca tggcgatgcc
    16081 ggtgacaccg acggcactgg tcaccgcgac gctgctggcg acgcaaggtg ccgccctgac
    16141 ctcgcgacag gtcacggtcg cgctcagcca ggtcctggac tatctcgaga acctcgccgc
    16201 cccgctggcc tcgagcgtgc caaacctgcg cgcggaagcc ggcgtcgagc aggtgctgcg
    16261 cgctctcgcg gcgtccggca tcgtcacgcg cgtcgagggc gcacgggacg tcgtctggct
    16321 catcggcccg gacaagcaac tcgccgcgac cttctatcgc aactcgatca tccagttctt
    16381 ccagctcggc gccatctgcg agatcgccct ggcgctcgcc gcccgatccg actccggcac
    16441 aaagcgattg gagcggttct ggcgcgcggt cgaccgcctg cgggatctgc tgaagttcga
    16501 cttctacttc caggagaagt cggtctatcg cgagcagatc gccgcgcaga tggcgatggc
    16561 cgaccccggg tgggaggcga aattgaccgc cgccgacttc gacatccgca cgatgctgcg
    16621 cggccggatg ccgctcgtcg cacccgtgag tctccggccg ttcttcgagg cctacggcat
    16681 cgtcgccgac gtgctcgccc accatggcgc agcgggaccg atcgacaagg cccaggtcac
    16741 caaggacgcc ctcgcctacg gcgagcaact gatcgcccag cgccgggtcg cctcggcgga
    16801 cccggtctcg gcgctcctct tcgccaccgg cctgcaactg gcggacaacc aaggggtctt
    16861 ggtagacggc ccggaccgtt ccgagcgggc cgcccgcttc gagcaggagc tccgcgagat
    16921 caccgaagcc gtcgccctca tcgcggccgt ctccacagag gtcttcaccg agcagatgcg
    16981 cgccgcccgg gcgtgaaacc cgttccctcc caaggaacct ctgcttggat caagacctgg
    17041 aacaccgacc ccaaaccctt catctggacc aaaaccgccg aagacatcct caaatccctc
    17101 ggaagacttc tcattagaac ttccggcgcg ggacactagc agaggttcct cggttggcca
    17161 gggagtgctg gaaggggagt atgcggcgga tcgcctcggc gaccgcgtcc gggccccggg
    17221 ccagcgacaa ccggatgtat tcgtgtccgt gcgccccgtc gaagtcagtg ccgggggtga
    17281 cggcgacatc ctgttcggcg agcagcgccg agcaccaggc gaccgaatcg gcatatggcc
    17341 caagctgatc gcggatcccg gcatacacgt agaaggcccc atcggcgggg gcgacgccgg
    17401 cccagccgag tcgcgggatg gcatcgagga cgatcttgcg ggcgagcgcg aatccggcaa
    17461 cggcgcgttc gcactcggca tacgactcct cggtgaaccc ggcgaccgcc gcatgctggg
    17521 cgggcaccgg cgcgcagagg gcgagattgc cggcgagggc gtcgatccgc ggtgcgaggt
    17581 cagcgggcat gagcatccac ccgaggcgcc acccggtcat gccccagtac ttgctgaagg
    17641 aagagatcac gacggcgttc tcatccaact gccaggcgca ggtgccgacc gagccggtga
    17701 aggtcacacc gtgatagatc tcgtcgctga cgacgcggac ctcgttgtcg cggcaccacg
    17761 tgaggatcgc ctcgagttcg gtgtcggcga tcatcgtgcc ggtcggattt gccgggctcg
    17821 ccaggatgac gccggtcagc ggtgcctggg tgtgcgccgc cgccaagagt tcggccgtcg
    17881 gctggaagcg cacctcttcg ccggtgtcca actcgacgac ctcgcacccg agcgagcgca
    17941 ggatgttgac atatgccggg tagccgggcc gggcgaccgc cacccggtcg ccggggtcga
    18001 aggcgctcaa gaaggcgagg aggaaggccc cggagctgcc ggttgtcacg cagatccgct
    18061 cggggtcgag gtcgaggtcg taccacttca ggtagtgacc ggcgatggcc tgacggagcg
    18121 cgagcagacc gcgagtctcg ctgtagccca acggaatctc gttgatcatg aggtgagcgg
    18181 cgacgtcgcg caccggcccg ggcgcgccct gcccgggctc gccggcgcac agactgatga
    18241 cctcgcgtcc ctgggcgcgg gcctcggcca cggcttgcag caccgtcatc acggtgaacg
    18301 gcgggacctc agagcggcgg gagggggtca gcggcatacg cgcgagtgta tgaggcgtta
    18361 gacgttggcg aggctgcgtc gggagcggcg caggccggcg ttcaaggaca cggtgacgag
    18421 caccgacgcc gcgacgagga ggcccatcga ggtagcgggc gacagcacgg tggcgagcag
    18481 cccaccggcc agggcgccga gggactgttg tcccaccatc tgcccgttgc cgaagagtcc
    18541 gaacacctgc ccttcgacgt ccggatccgc ctcatgggcg agccgctcct gcaagacaag
    18601 ggaagccgag tagccggcgc tggcgacgaa tcccaggacc aacgccacgc cgaccggcgg
    18661 gtggaagagg aagccgaggt agggcagtgc cagcagcggg cgcagcggtg tcagcagccg
    18721 gtcccggcgg tcggtggtca gtatgcggcc gacgacgatg tcgccgagga gcatcccgcc
    18781 gctgcggccg cgaagaggca gccggaccac gctttgcctc cgtagggaat gaagagggct
    18841 tcacatccga cgatgaggcc catgggaatc cacgcgctga gcatgatcgg gcgcaggatg
    18901 ggcgagccca gcacatgggc attgacccgg tgggtctcgg cgaggatatt ccccggacgc
    18961 gcaggcatgg gtcgggccct gacccccgcg agcagcacgg cgaaccgcat gggcaacggc
    19021 aacctaggga cggcctgcaa tgcggcttgc gctccggtga cggcgatgac gaggaggatg
    19081 gcgctgcgcg cgtccagccc gtcgaacagg ctcagggcgg tcagcgatcc cagcagcgtc
    19141 agcagcgaac cgccgacgag ggccagcgca ctgagaatgg gcgacccggt ctcctcgtag
    19201 gcgagcgtgg ccagggcgag cgaggccaag ctgaggctgg cgatgacgag gcagcgggag
    19261 acgaacaggg ctcggaactc gccgatggcg aagagttcgc cgtaggtgcg catgccggcc
    19321 agcctgtggg ggactgggtc g
//
LOCUS       DAOPJG010000041        18728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3105_length_18728_cov_7.848337, whole genome shotgun sequence.
ACCESSION   DAOPJG010000041 DAOPJG010000000
VERSION     DAOPJG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 18728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18728
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3105_length_18728_cov_7.848337"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PKU75_05705"
     CDS             <1..793
                     /locus_tag="PKU75_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPF80409.1"
                     /translation="AATLTGSERAGVAVGSAAGAALKKVVLVLGGSDPFVVLDDADLD
                     AAADAAIRSRFGNAGQSCIAAKRWIVQESVADDFVSRIQDRMARLVAGDPADPATNIG
                     PMARDDLRRAVHEQVQDSVTEGATLVAGGVIPDGPGYHYPATLLDNVSTSMTVAREET
                     FGPVACVIRVADDDEAVRVANDTAYGLGASVWSRSERGLAVGTRIRSGALFVNAVVAS
                     DARMPFGGLGRSGHGRELGAAGVREFTNVRTVWIGDGAHLPTIAE"
     gene            813..1247
                     /locus_tag="PKU75_05710"
     CDS             813..1247
                     /locus_tag="PKU75_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF80410.1"
                     /translation="MISGRFDHVGLNVTDLPGMTRWYCDTLGMEIFFEFALDHVDFHG
                     VMLRHPDGYHIELVHRPGATTGIQATNPVEAALTLGYSHFALDVPEVDPVYDALIAAG
                     ASDRMSPRPSPEPGVRMAFVADPEGNLIELLDRTAVRMREAA"
     gene            1244..2017
                     /locus_tag="PKU75_05715"
     CDS             1244..2017
                     /locus_tag="PKU75_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF80411.1"
                     /translation="MTGRLDGKVALITGVGGGMGVEAARLFCAAGAKVVGCDFFEEGA
                     RQTEEAVRAEGGEITVMGGVDLGDAQAAKAWIDQAAEVYGGFDILYNNASTQRFAGIE
                     ELSVEDWDYTMRNELNLVFYACKAAWPHLKKRGGGVILNVGSIAGLRGVEFMPQNAHG
                     TAKAGVMSLTRQLVVEGAPHGIRANTISPGLIETPNTRTFLADPPENVKRIVLDRIPV
                     GRVGQPADIVNAAIFLASDDATYINGANLVIDGGGSVLG"
     gene            2114..2347
                     /locus_tag="PKU75_05720"
     CDS             2114..2347
                     /locus_tag="PKU75_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80412.1"
                     /translation="MKTTIEIPDALAAEAKQVARDEGSTLRDLVVTGLRAEVDRRRRR
                     GVVDFVFPSFGGDGLLLDVAPEGMIARSYGLSE"
     gene            2344..2781
                     /locus_tag="PKU75_05725"
     CDS             2344..2781
                     /locus_tag="PKU75_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPF80413.1"
                     /translation="MIALDTNLLVYAHRRDSAHHAAAAAAISAAVLHRGGCAVPWPCL
                     HEFLAVVTHPRIYSPPTPPAEAIAAVVALAAQPGVHVIGEGTDHLTVLSDLLSSGRVT
                     GPKIHDARVAAICLAHGVDALWSADRDFSWFPALRVVNPLVTA"
     gene            3067..3390
                     /locus_tag="PKU75_05730"
     CDS             3067..3390
                     /locus_tag="PKU75_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80414.1"
                     /translation="MQALAQAFVDNERFLRAVVLVSSQDPEIERRGVQYVARLSARFA
                     AALHPARPAMRHSDPHAAIRWIFGDLFAALTLRTAYGPTFFGPAHTDGQHLEHLCVLA
                     RAFLL"
     gene            3525..4475
                     /locus_tag="PKU75_05735"
     CDS             3525..4475
                     /locus_tag="PKU75_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF80415.1"
                     /translation="MSSHLNEHGITHLRHVDLAVPDYETQRTFYKDTWGLTEVGTDGN
                     LSYLAAEGSPEQYVVRLRQAAEKRLDLISFGAESRAAVDALAAHLGSQGVQLIGEPGD
                     LQTMGGGYGIRFFDIDGRTIEVSADVATREHRKIEEGEAIPVRISHAVMNSNNLAATK
                     EFYTDVLGFKLSDSLWSAHMGDMMHFLRVNDWHHSLAIATGPHPSVHHVSFEMRGLDE
                     YMRGTGRVMRAGIKKVWGPGRHLAGNNTFSYFIDPSGNTMEYTTELEKIDEDQWHPSV
                     YDIAEPMTQDQWGTADPMSEIIAKESFNDPDRGLFVAPPV"
     gene            4475..5281
                     /locus_tag="PKU75_05740"
     CDS             4475..5281
                     /locus_tag="PKU75_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF80416.1"
                     /translation="MTTGGRCAGKIVVVTGGARGQGAAEVEDLAREGASVYAVDLLDE
                     EGRALADRLTEEGYDVAYGHLDVTDPADWEALAAELSARHGRVDALINNAGVASRGRL
                     PYVDLDVWHRTFEINVTGPLLGIQALVPLMTQGGSIVNICSVAAISGHVAAPYTASKW
                     ALRGLTRTASLELGPKGIRVNAVMPGLIETPLMESASPEFRGAALREIPLGRTGEVGD
                     IAPLIVHLVSDESRYTTGAEIVVDGGMSAHVSHKGVADAIAPAAPPEAAS"
     gene            5278..6234
                     /locus_tag="PKU75_05745"
     CDS             5278..6234
                     /locus_tag="PKU75_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPF80417.1"
                     /translation="MKFGTVRFADGVQGGVVVGADGQSVHPLGRDLESVLLEADTLEA
                     VLRVGAVASERPAVSLSDVTVRKPFTPAAIRDFVAFEEHVVGVRKSVDGSSGVPEQWY
                     AAPTFYFTNPHAIYGPDEPIARPDLCRALDFELEVAAVIGRQCRSVDEAQARDAIIGY
                     TIFNDWSARDLQSAEMKVGLGPAKGKDFASSLGPWLVTADELEDHRDADGFLELDCSV
                     SVNGVEVGHDSLANMGWTFETMVAYASRDSIVKPLDILASGTTGGGGCLAELWGRGGS
                     QHPPSLQPGDLVTITVDGLGTLSTPIVAGAPTPELTPFRRRS"
     gene            complement(6252..6920)
                     /locus_tag="PKU75_05750"
     CDS             complement(6252..6920)
                     /locus_tag="PKU75_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80418.1"
                     /translation="MTAADEDRYAERVHRLCVPIALRPGGRVWIGPLLPDDRDLLSRE
                     YATLSPESKWNRFLAAVPELTPSMLDQLVDEVDGVDHVALVAFLETQAGLVPAGVGRL
                     VRYPDHPGAADMAFTVKDEYQGRGIATALARSLVARAPKGVTRILTEVAHGNLAPLAI
                     LGKLGSMQVFQSSEGVVDVEVKLAGHEEDSAAESTCLHRRPGIPSPAHPRLDAQHHGL
                     PPTA"
     gene            7073..7216
                     /locus_tag="PKU75_05755"
                     /pseudo
     CDS             7073..7216
                     /locus_tag="PKU75_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            7372..8385
                     /locus_tag="PKU75_05760"
     CDS             7372..8385
                     /locus_tag="PKU75_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPF80419.1"
                     /translation="MTKQMRAWSVGAPGPIAERPLVETTLPVPQPGPGEVLVAVSVCG
                     VCRTDLHVAEGDLAVHRAGVVPGHEVVGQVVARGAGASRFAEGERIGIAWLRSTCGSC
                     QACRAGRENLCPHSQYTGWDADGGYAEYTVVPQSFAYRIPEWYDDEAAAPLLCAGIIG
                     YRALRRASVPPGGRLGIYGFGGSAHITAQIAIAQGIEVHVLTRGEAARQLALKLGAAS
                     AGAAYDAPPVPLDSAILFAPVGDLVPVALAGLADGGTLAVAGIHLSDVPALNYQQHLF
                     RERTLTSVTSNTRRDGEELLTLAARLGVRPTTTAYPLARADAALSDLACGRVTGAAVL
                     HTG"
     gene            8494..9648
                     /locus_tag="PKU75_05765"
     CDS             8494..9648
                     /locus_tag="PKU75_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPF80420.1"
                     /translation="MFEYFTGPKYIWNMGVSATLNSGGQIFEVDKASRPAKEAADVDS
                     GAPLFLQGWKAVADDLAEQAKEAEAAGHLRTAGQKWFRSGVYVCQAERMLSESMPERR
                     AFYQQALDAMQKTFDLIDPNTSRVEVPFEGASLPAYFTKAVGTDGPAPTVIMWNGLDS
                     TKEHMYTSGWPTEMSQRGYNVLQVDCPGSGEALRFRGLKARISTEDWAKACVDYLEGR
                     EDVDLAKVGLVGWSLGGYYVPRAAAFEKRLAYAVAWGANHNWGEVQRGRLEREGENPV
                     PHYWEHVLWVWGFEGKTPQEFVEFADDVHLNGIVEKITCPFLIAHGEGDRQINVKYAH
                     QSYDQAVNSPKRTLRLFTAEEGATEHIGLDHLSHVGNYISDWIDDTLAGK"
     gene            complement(9740..10147)
                     /locus_tag="PKU75_05770"
     CDS             complement(9740..10147)
                     /locus_tag="PKU75_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF80421.1"
                     /translation="MPARTAIATALLVRDGLVLMAHRHPARRWYPDCWDLVGGHVEPG
                     ESPRQAVIRECREELAIEVHDPVPIPMTFDDPTLDMHAFLVTRWDGEPLNVEPEEHDD
                     LRWFRPDELAGLTLADPASLPSILRAVSSAAPQ"
     gene            10885..11013
                     /locus_tag="PKU75_05775"
     CDS             10885..11013
                     /locus_tag="PKU75_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80422.1"
                     /translation="MGSLVLTHFWPGNDREATRAAAAAHFAGPIALAEEGMVISLG"
     gene            11105..12670
                     /locus_tag="PKU75_05780"
     CDS             11105..12670
                     /locus_tag="PKU75_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPF80423.1"
                     /translation="MRTFPDGFLFGVSTAGHQIEGNNTTSDTWFLEQQQPSVFTEPSG
                     PACNSFELWESDLDLVASFGLNAYRFSVEWARVEPEEGRFDDAAIDHYEAMVDGCVAR
                     GLKPVVTLNHFTSPHWFACRAGWLDPEAPELFARYVAKVIARFGERLAFVITFNEPDL
                     PAMLQWANLPPVVAELERATLDAASASAGVERYRAANVVLPEDMHAIADGMTAGHRAA
                     KAIVKDRYPELPVGFSLAMVDDVVVGDDPSVRDRKRAEVYGRWLELAREDDFLGVQNY
                     ERLTYDGNGLVLPEGAASGALYGGSEPRSLAECVKYANSVAGVPILVTEHGMEGQDDA
                     KRVAFIEDSLLHLLDVIDSGVAVLGYLHWTLLDNFEWIFGYEKTLGLVAVDRETFART
                     PKPSAAALAAIATGRGVPDPVTSEALVATDRPGRYGKQLASHLSKRVASQWDDETGTG
                     SLVFGHGGRADLAAVDGGLRFELVARPASVARLEDVIGRHLVRFGARDELAVAWTRSD
                     GTAGTTQRNSEDD"
     gene            12727..13266
                     /locus_tag="PKU75_05785"
     CDS             12727..13266
                     /locus_tag="PKU75_05785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPF80424.1"
                     /translation="MAAGDRPRRVVTGHTPDGVSVVVSDGPVPVSRALPDDGVFFHEI
                     WNTQDAPAPVRAVEPVEPTEADLTVPPPPRGTKIRINEFLPGHLDERGLQSPVHRTQS
                     IDYGIVLEGEITLILDDTEVTLRAGDIVVQRGTDHAWANRSDGITKVAFILVDGQFDP
                     VLAESIGGLDGLMHGGPRD"
     assembly_gap    13324..13423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13424..13792)
                     /locus_tag="PKU75_05790"
     CDS             complement(<13424..13792)
                     /locus_tag="PKU75_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPF80425.1"
                     /translation="MALADAPRRRLGPFEVAPIGLGCMNLSHVYHPIPSPEQGAALIA
                     AALDAGVDHFDTAALYGFGANEDLLGATLGSRRADITLASKCGMYGRDGKRVIDGAPE
                     SIRRLCDEALTRLRTDVIDLY"
     gene            complement(13792..14769)
                     /locus_tag="PKU75_05795"
     CDS             complement(13792..14769)
                     /locus_tag="PKU75_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF80426.1"
                     /translation="MTDQPLRIALAGGGAFGAKHVNGLKKIDGVELAAIISGSVESAQ
                     GFAAEHGVGRAVESLDDVLAMDDIDAVILATPTPMHASQTLACLRAGKHVQVEIPLAD
                     SLPDAEECLRVQQESGLVGMVGHTRRFNPSHQWVHNKIAAGEFHIQQMDVQTYFFRRK
                     NLNALGQPRSWTDHLLWHHAAHTVDLFAYQAGSPIVQAHAIAGPSHPDLGIAMDMSIQ
                     LKAENGAICTLSLSFNNDGPLGTFFRYIGDTGTYIARYDDLTDGKDEAIDVSKVDVSM
                     DGIELQDREFVAAIREGREPNSSIAQVIDCYRVLGDLEQQVDPQREAHG"
     gene            complement(14771..16141)
                     /gene="ligA"
                     /locus_tag="PKU75_05800"
     CDS             complement(14771..16141)
                     /gene="ligA"
                     /locus_tag="PKU75_05800"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013603154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HPF80427.1"
                     /translation="MALDKEYRKVPGTTIFDSEQSAKGYHLNQFCMSLMKAENRERFL
                     ADEDAYMDEWPLTDAQKKALRDRDLNAAMREGGNIYFLAKFGATLGLSFQQMAGSMTG
                     MTEQEYRDMMVGGGRSIAGNRIADVAVPEVERTEAEIAAAQEQDPMLDAVRADAAADD
                     SVGNATITGAVFTSHVPAIGAALDLGKTEEPYWKAVFDGYEFSKAWEQDNLPDVVFLV
                     YNDHATAFDLSLIPTFVLGTGAAYPAADEGYGPRPVPGIEGDPDFAAHVAHSLIRDDF
                     DLTLVNEMEVDHGLTVPMSLMFGQVDTWPVKVIPFHVNVVQYPVPSGERCFKLGKALR
                     KAIESYEKPIKVQIWGTGGMSHQLQGPRAGLINREWDNAFLDRLIEDPEGLSHVPHLE
                     YVEEAGSEGIELVMWFIARGAMSDVEGDGEIEVVHRFYHVPASNTAVGHLILNNKPPA
                     AAAAKE"
     gene            complement(16145..17077)
                     /locus_tag="PKU75_05805"
     CDS             complement(16145..17077)
                     /locus_tag="PKU75_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPF80428.1"
                     /translation="MSTPQKTEFEKTPGWLDWYAGPSTPRFRLPAGAVDAHCHVFGPG
                     EEFPFAPERKYTPCDASKEQLYALRDHLGFARNVIVQATCHGADNSAMVDAIQSSGGK
                     ARGVATVRPGVAQDELQRLHDAGVRGVRFNFVKRLVDVLPMDGLQKVADQIGPLGWHI
                     VIYFESQELPELADFFLGLPVPLVVDHLGRPDVTTDVDGPGFTGFLDLMRARDDIWCK
                     VTCPERLTTTGPRALGGEQAAYRDVVPFAKRVVEEFPDRVLWGTDWPHPNLKDHMPDD
                     GLLVDWIPSIATAEESQRKLLVDNPMRLYWPEEG"
     gene            complement(17074..17772)
                     /gene="ligK"
                     /locus_tag="PKU75_05810"
     CDS             complement(17074..17772)
                     /gene="ligK"
                     /locus_tag="PKU75_05810"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HPF80429.1"
                     /translation="MNPNELGVVYTGTPRPDAAAVEALSAFGVATIHEAMGRTGLMRP
                     YIRPAFPGARICGPAVTVLLQPGDNWMFHVATEQVGPGDVLVAACTTESEDGFFGELL
                     ASSLRARECGGLIIDGGVRDVADLEKMNFPVFSRAINSKGTVKATLGSVNIPIVCANA
                     IVNPGDVVVADVDGVVVVPRDRAAEIAEAAKTREDNEESKRAKFREGVLGLDLYNMRE
                     PLEKAGLTYKEWPA"
     gene            17981..18433
                     /locus_tag="PKU75_05815"
     CDS             17981..18433
                     /locus_tag="PKU75_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80430.1"
                     /translation="MCEFHSATIVHAFTAAWEYQCCGTPVSVGDELTLRHVAWDEGLP
                     GNDAYRRVQHLDWYVGHHVTSEEEGAAEDLVRVLALWEAHSDLDFRAGDDGYYAAARD
                     SGRLVPITTMVPFDATWAGDGPPTPPARHTPREPQGWIMKLEVLRRDG"
     gene            complement(18446..>18728)
                     /locus_tag="PKU75_05820"
     CDS             complement(18446..>18728)
                     /locus_tag="PKU75_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPF80431.1"
                     /translation="ALLRNVFFDTCVYHQPGIDLLTSVIPSESILFASEMIGAVRGID
                     SRTGHYFDDTKRYVDATPNLTDEQRTAVYEANARRAYPRLDATLTARGR"
BASE COUNT         2980 a         6238 c         6340 g         3070 t
ORIGIN      
        1 cgccgccacg ctcacgggca gtgagcgcgc cggggtcgcc gtgggatcgg cggcgggtgc
       61 ggcattgaag aaggtcgtgc tcgtgctcgg aggctccgac cccttcgtcg tcctcgacga
      121 cgccgacctc gatgccgcgg ccgatgcggc catccggtcg cgcttcggca acgccggcca
      181 gagctgcatc gccgcgaaac gctggatcgt ccaggagtcc gtcgccgacg acttcgtgtc
      241 ccggatccaa gaccggatgg cgcgcctggt ggcgggcgat cccgccgacc cggccacgaa
      301 catcgggccg atggcccgcg acgacctgcg ccgcgccgtg cacgagcagg tgcaggactc
      361 cgtcaccgaa ggcgccaccc tcgtcgccgg cggcgtcatt cccgacggtc ccggatatca
      421 ctaccccgca acacttctcg acaatgtgtc gacatcgatg acggtcgccc gcgaagagac
      481 gttcgggccg gtggcctgtg tgatccgggt ggccgacgac gacgaggcgg tgcgcgtggc
      541 gaatgacacg gcatacgggc tcggtgccag cgtctggtcg cgatccgaac gcggcctcgc
      601 ggtcggcacc cgcatccgct ccggcgcgct gttcgtcaac gcggtcgtcg cctcggacgc
      661 ccggatgccg ttcggcggtt tggggcgctc gggacacggc cgagagctcg gagccgccgg
      721 cgtccgtgag ttcaccaatg tgcggaccgt ctggatcggc gacggcgccc acctgcccac
      781 catcgcggag tagccatgac ctggaggagc caatgatcag cggacgattc gaccacgtcg
      841 gcctcaatgt caccgacctg ccgggcatga cccggtggta ttgcgacacg ctgggcatgg
      901 agatcttctt cgagttcgcg ctggaccacg tggacttcca cggagtcatg ctgcggcacc
      961 cggacggcta tcacatcgag ttggtgcacc gtcccggtgc gaccaccggc atccaggcca
     1021 cgaaccccgt cgaggcggca ttgacgttgg gctacagcca ctttgcgctc gacgtgcccg
     1081 aggtcgatcc tgtctacgac gccctcatcg ccgccggcgc gagcgaccgg atgtcgccgc
     1141 ggccctcccc ggaaccgggc gtgcggatgg ccttcgtcgc cgaccccgag ggcaacctga
     1201 tcgagctgct cgatcgcaca gcggtccgga tgagggaggc agcatgacgg ggcggctcga
     1261 cggaaaggtc gcgctcatca cgggcgtcgg tggcgggatg ggcgtcgagg cggcgcgcct
     1321 gttctgcgcc gcaggcgcca aggtggtcgg gtgtgacttc ttcgaggaag gggcgcgcca
     1381 gaccgaggag gcggtccgtg ccgagggcgg cgagatcacg gttatgggcg gggtcgacct
     1441 cggtgacgcg caggccgcga aggcgtggat cgaccaggcc gccgaggtct acggcggctt
     1501 cgacatcctc tacaacaacg cctccactca gcgcttcgcc ggcatcgagg agttgtccgt
     1561 cgaggactgg gactacacga tgcgcaacga actcaacctc gtgttctacg cgtgcaaggc
     1621 ggcctggccg catctgaaga agcggggcgg cggcgtgatc ctcaacgtcg gctcgatcgc
     1681 cggtctgcgg ggtgtggagt tcatgccgca gaacgcgcac ggcacagcca aggcaggcgt
     1741 gatgtcgctg acccggcaac tggtcgtcga gggtgcaccg cacggcatcc gcgccaacac
     1801 catcagcccc gggctcatcg agacgccgaa cacccggacc ttccttgccg acccgccgga
     1861 gaacgtcaag cgcattgtgc tggaccggat tccggtcggg cgagtcgggc agccggcgga
     1921 catcgtcaac gccgcgatct tcctcgcatc ggacgacgca acctatatca acggcgccaa
     1981 cctcgtcatc gacggcggcg gttcggtcct ggggtgaggc catacgcgat ctgaccgagg
     2041 acgagagggt gaggttgctg ctgagactgc actcggcata cgggagtacg ggtccattga
     2101 tacggtagac cgtatgaaga cgacgatcga gatcccggac gccctggcgg cggaggccaa
     2161 acaggtggcg cgcgacgaag gctcgacgct gcgcgacctc gtcgtgacgg gtttgcgcgc
     2221 cgaggtcgat cgtcgccgcc ggcggggcgt ggtggacttc gtctttccca gtttcggggg
     2281 tgacgggctg ctgctggatg tggcgccgga ggggatgatc gctcgctcgt atgggttgtc
     2341 ggaatgatcg cgctcgacac gaacctcctc gtctatgccc accgccgcga ctcagctcat
     2401 catgcggctg cggctgccgc gatctcggcg gccgtgctgc atcgcggggg ctgcgccgtg
     2461 ccgtggccct gtctgcacga gttcctcgct gtggtcacgc atccgcggat ctactccccg
     2521 ccgacgccgc ccgcggaggc gatcgcggcc gtggtggcgc tggccgcgca gcccggtgtc
     2581 cacgtcatcg gtgaggggac agatcacctc accgtgctgt ctgacctgct gagcagcggg
     2641 cgcgtgacgg gtccgaagat ccatgatgcg cgggtggcgg cgatctgcct cgcgcacggt
     2701 gtcgacgcgt tgtggtcggc tgatcgcgac ttctcctggt tccccgccct ccgcgtcgtc
     2761 aatccgctgg tcacagcctg accacgggcc gggggaacgt gcggccgcca gtggccggaa
     2821 tacgtgacga cgttggtgag gtggagagtt gaccgcaagg tatgccgtgc gcgaaccccg
     2881 tcaggagcgc acccgcgtcg cgtggcagcg catcctggac gcgggggtcg ccgtgctcga
     2941 agacgagggt gccggcgggt tcaccatcga tgcgatctgc gcccgggcga gcgtcgtccc
     3001 gcgggccgtc tatgaccggg tcgaccgcac caggacgttc cacccgccgc tcgcatccat
     3061 gccaccgtgc aggcacttgc tcaggccttc gtcgataacg aacgattcct gcgcgccgtc
     3121 gtcctcgtgt cgagtcagga tcccgagatc gagcgccgcg gagtgcagta tgtcgcgcgg
     3181 ctctccgcgc ggttcgccgc ggctctccat cccgctcgcc cggcgatgcg gcattccgat
     3241 ccgcacgccg cgatccgctg gatcttcggc gatctcttcg cggcgctcac cctgcgcacg
     3301 gcatacggtc ccaccttctt cggccccgcc cacaccgacg gccaacacct ggagcacctc
     3361 tgcgtgctgg cgcgcgcctt ccttctctga cacgcgggca ctcgtccgga gcgctggcgc
     3421 aacgggcgat atctgccatc actggagacg agttccgccg cgagccgggc agccataccg
     3481 tcgagaccat ccctgaagca ggcacgcgga gcgaggaagc gaccatgtcc agccacttga
     3541 acgagcacgg catcacccat ctgcggcacg tggacctggc ggtgccggac tacgagaccc
     3601 agcgcacctt ctacaaggac acctgggggc tgacggaggt cggcaccgat ggcaacctct
     3661 cctacctcgc cgccgagggt tcgcccgagc agtatgtggt gcgcctccgc caggccgccg
     3721 agaagcgcct cgacctgatc tccttcggcg ccgagagccg tgcggcggtc gacgcgctgg
     3781 cggcgcatct cgggagccag ggggtgcagc tgatcgggga gcccggcgac ctgcagacga
     3841 tgggcggcgg ctacggcatt cgcttcttcg acatcgatgg ccgcacgatc gaggtctccg
     3901 ccgacgtagc gacccgcgag caccggaaaa tcgaagaggg agaagcgatt ccggttcgca
     3961 tctcgcacgc cgtcatgaac tccaacaacc tcgcggccac caaggagttc tacaccgacg
     4021 tcctcggatt caagctctcc gacagcctgt ggagcgcgca catgggcgac atgatgcatt
     4081 tccttcgcgt caacgactgg caccacagcc tggcgatcgc caccggcccg catccgtcgg
     4141 tgcaccacgt ctccttcgag atgcgcggcc tcgacgaata catgcgcggc accggacggg
     4201 tgatgcgcgc cggcatcaag aaggtctggg gccccggtcg tcacctcgcc ggcaacaaca
     4261 ccttctccta cttcatcgac cccagcggca acaccatgga atacaccaca gagctcgaaa
     4321 agatcgacga ggaccagtgg catccgagtg tctatgacat cgccgagccg atgacccagg
     4381 accagtgggg cacggccgac ccgatgagcg agatcatcgc caaggaatcc ttcaacgacc
     4441 ccgaccgcgg cctcttcgtc gcgccgccgg tctgatgacg accggcgggc gctgcgccgg
     4501 caagatcgtc gtcgttaccg gcggcgcgcg cgggcagggt gccgccgaag tggaagacct
     4561 tgcccgagaa ggtgcttcgg tgtatgccgt cgacctcctc gacgaggagg gtcgcgcgct
     4621 cgcggatcgg ctgaccgaag aggggtatga cgtcgcctac ggccacctcg acgtcaccga
     4681 tccagccgat tgggaggcgt tggcggccga gctgtcggcg cgtcatgggc gagtcgatgc
     4741 gttgatcaac aacgccggcg tggcttcgcg tggacgcttg ccgtatgtcg atctggacgt
     4801 gtggcaccgc accttcgaga tcaatgtcac cgggccgttg ctcgggatcc aggcactcgt
     4861 gccgttgatg acgcagggcg ggtcgatcgt caacatctgc tcggtcgccg cgatctccgg
     4921 acatgtggcc gcgccctaca cggcctcgaa atgggccctg cgcgggctca cgcgcaccgc
     4981 gtcattggag ttaggcccca agggaatccg cgtcaacgca gtcatgcccg gtctcatcga
     5041 gacgcccctg atggaaagcg cctcgccgga gttccgtggc gcggccctac gtgagattcc
     5101 actcggacgc accggtgagg tcggcgacat tgccccgctc atcgtgcatc tcgtctccga
     5161 cgaatcgcgc tacaccacag gcgccgagat cgtcgtcgac ggcggtatgt ccgcacacgt
     5221 atcgcacaag ggggtcgccg acgccatcgc gcccgctgcc ccgcccgagg cggcatcgtg
     5281 aagttcggaa ccgttcggtt cgccgatggc gtgcagggtg gcgtcgtcgt cggtgccgac
     5341 ggacaatccg ttcacccgct gggtcgcgat ctggagtcag tcctcctcga agcggacacg
     5401 ctcgaggccg tgctgcgcgt cggcgcggtc gcgagcgagc ggccggccgt ctcactgtcc
     5461 gacgtgaccg tgcgaaaacc attcacccct gcggcgatcc gtgacttcgt tgccttcgaa
     5521 gaacacgtgg tcggcgttcg caagagcgtc gacggcagca gcggagtccc cgagcagtgg
     5581 tatgccgcac ccacgttcta cttcaccaac ccgcacgcca tctatggccc ggacgagccc
     5641 atcgcccggc cggatctgtg ccgggccctc gacttcgagt tggaggtcgc ggccgtcatc
     5701 ggacggcagt gccgttcggt cgatgaggcg caggcgcgcg acgcgatcat cggctacacg
     5761 atcttcaacg actggtcggc gcgcgacctc caatccgccg agatgaaggt cggcctcggc
     5821 cccgccaagg gcaaggattt cgcgagctct ctcggcccct ggctggtgac cgccgacgaa
     5881 ctcgaggacc accgcgacgc cgacggattc ctcgaactcg actgcagtgt cagcgtcaat
     5941 ggtgttgagg tgggccacga ttcactcgcc aacatggggt ggaccttcga gacgatggtc
     6001 gcctacgcct cgcgcgactc gatcgtcaaa ccgctcgaca tcctcgcctc tggcaccacc
     6061 ggtggcggcg gctgcctagc ggaactctgg ggccggggcg gctcgcagca cccgccatcg
     6121 ctgcaacccg gcgaccttgt gaccatcacc gtcgacgggc tgggcacgct gtccaccccg
     6181 atcgtcgcgg gcgcgcccac tcccgaactg acacccttcc gccggcgttc ctgacgccca
     6241 gcggggcttg atcacgccgt cgggggcagc ccgtggtgct gtgcgtccag tcgcggatgt
     6301 gccggggagg gtatgccggg acgccggtgc aggcaggtgc tctcggccgc cgagtcctcc
     6361 tcgtggccag cgagtttcac ctcgacgtcg accacgcctt cggagctttg gaacacctgc
     6421 atactgccga gcttgccgag gatggccagc ggggccaggt ttccgtgggc gacctcggtc
     6481 aggatgcggg tcacgccctt gggcgcgcgc gccacaaggg accgggcaag cgccgtggcg
     6541 atgccgcggc cctggtactc gtccttgacg gtgaaagcca tgtccgccgc gccgggatgg
     6601 tccggataac gcaccagccg gccgacgccc gccgggacca ggccggcctg ggtctcgagg
     6661 aaggcgacga gcgcgacgtg gtccacgccg tcgacctcgt cgacaagctg gtccagcatc
     6721 gacggagtca actcgggcac cgcggcgagg aatcggttcc acttcgattc tggagacaag
     6781 gtcgcgtact cgcgcgacaa caggtcgcga tcgtccggta acagcggccc gatccacaca
     6841 cgcccgccgg ggcgcagcgc aatcgggacg cacaggcgat ggacccgctc ggcgtagcgg
     6901 tcctcgtccg cagcagtcat ggattgagct cgattcccct cggttaggcg catttcagcg
     6961 tccacgtgct ttctacggta agcacataga gcaagcgagc aagcggagga aaggtgtggt
     7021 ctccgtctcg cggcgcgtgg taccagggcg gtaccatcag gcgtggccat gaatctacgg
     7081 ctcaccgccg acgagaccga cgcgctctgc cgacgcatat ccaaccctct gcgagaaagc
     7141 agctgcccta ctgcattccc tcgccccgaa tcacccgttt actgaaggca acacgcgtct
     7201 cgcgtgggca ggcgctcggg tcttcctctt gttgaattcg tgcgacgtcg gctacgcatc
     7261 cgtgggtgag gtcgagggct tcgtgctcgc ggtggccaga ggcgaactcc aggtccccga
     7321 gatcgccgcc tggatcgaac accatctgac gctggacgag agcgggcagc aatgacgaaa
     7381 cagatgcggg cgtggtcggt cggagcgccc ggcccgatcg ccgagcgacc gctggtcgag
     7441 acgacgctgc ccgttcccca gccggggccc ggagaggtcc tggtagccgt gtccgtctgt
     7501 ggggtgtgcc ggacggatct gcatgttgca gaaggagatc tggcggtgca tcgggccggc
     7561 gtcgtgcctg ggcacgaggt cgtcggccag gtggtcgctc ggggcgccgg agcgagtcga
     7621 ttcgccgagg gtgagcggat cgggatcgcc tggctgcggt ccacatgcgg cagttgtcag
     7681 gcgtgccgag ccgggcggga gaatctgtgt ccgcattcgc agtacaccgg ctgggacgcc
     7741 gacggcgggt atgccgagta caccgttgtc ccgcaatcgt tcgcctatcg catccccgag
     7801 tggtatgacg acgaggcggc cgcgccgctc ctgtgcgccg gcatcatcgg gtatcgcgcc
     7861 ttacgccggg cgtcggtgcc gcccggcggc cggctcggca tctatgggtt cggggggagc
     7921 gcccacatca ccgcacagat cgcgatcgcc cagggcatcg aggtccacgt gctcacccgc
     7981 ggcgaggcgg cccggcaatt ggccctcaag ctgggagcgg cgtcggcggg agcggcatac
     8041 gacgcaccgc cggtgccact cgactcggcg atccttttcg cgcccgtagg ggatcttgtg
     8101 ccggtcgccc tggcggggct agccgacggg ggcaccctgg cggtcgcagg catccatctc
     8161 tccgacgtgc ccgccctgaa ctaccagcag catctcttcc gcgagcgcac actcacgagc
     8221 gtgaccagca acacccggcg cgacggcgag gaactcctca cgctcgcagc ccggctcggc
     8281 gtgcggccca cgaccacggc atacccgctg gcgcgcgcgg acgcggccct gagcgacctg
     8341 gcctgcgggc gggtcacggg cgcggcggtg ctgcataccg gctgaggctc cccggtgtgg
     8401 cgcgatacct cgcatcactg gtgcctgctt ccgcccggcg aggcaccggg gcacagtgtg
     8461 aagtgaatcg tccgcgagtg caggagagca gcgttgttcg aatacttcac cggccccaag
     8521 tacatctgga acatgggcgt cagcgcgacc ctgaactccg gtggtcagat cttcgaggtc
     8581 gacaaggcgt cgcggccggc gaaggaggcg gcggacgtcg actcgggcgc ccccctgttc
     8641 ctccagggct ggaaggccgt ggccgacgat ctcgccgagc aggccaagga ggccgaagcg
     8701 gccgggcacc tgcgcaccgc cgggcagaag tggttccgct cgggcgtcta tgtgtgccag
     8761 gccgagcgga tgctcagcga gtccatgccc gaacggcgcg ccttctatca gcaggcgctc
     8821 gacgcgatgc agaagacctt cgacctgatc gaccccaata cgagccgagt cgaggtgccc
     8881 ttcgaaggcg cctctctgcc agcgtatttc acaaaggccg tcgggaccga cggcccggcg
     8941 ccgacggtga tcatgtggaa cggcctggac tccaccaagg agcacatgta cacctccggg
     9001 tggccgacag agatgtcgca gcgtggctac aacgtgctgc aggtcgactg tcccggctcc
     9061 ggcgaggcgc tgcgcttccg gggcctgaag gcccgcatca gcaccgagga ctgggctaag
     9121 gcgtgcgtgg actatctcga gggccgggag gatgtcgacc tcgccaaggt cgggctggtc
     9181 ggctggtcac tcggcggcta ctacgtcccg cgtgccgcgg ccttcgagaa gcgcctggcg
     9241 tatgccgtcg cctggggcgc caaccacaac tggggcgagg tgcagcgcgg ccgcctcgaa
     9301 cgcgagggcg agaacccggt gccgcactac tgggagcacg tgctgtgggt gtggggtttc
     9361 gagggcaaga ccccgcagga gttcgtcgag ttcgccgacg acgtccacct caatggcatc
     9421 gtcgagaaga tcacctgccc cttcctgatc gcccacggcg agggggaccg gcagatcaac
     9481 gtcaagtacg cccaccagtc atacgaccag gccgtcaact cacccaagcg caccctgcgc
     9541 ctcttcacgg ccgaggaagg cgcgaccgag cacatcggcc tcgatcacct ctcccacgtc
     9601 ggcaactaca tctcggattg gatcgacgac actctcgccg gcaagtagcc acgcttcgct
     9661 cgaggggact ttgcgacaag gagtttcggt atccgtgtcg cgctccgtcg gttgacagct
     9721 gtcgtctgcg acgtctgact cattgtggcg cagcggaact cactgcacgc aggatgctcg
     9781 gcagactggc cgggtcggca agcgtgagtc cggcaagctc gtcgggacgg aaccaccgca
     9841 ggtcatcgtg ctcctcgggt tcgacgttga gaggctcgcc gtcccatcgc gtgaccagaa
     9901 aggcatgcat gtcgagggtg gggtcgtcga aggtcatcgg gatgggcacg gggtcgtgga
     9961 cctcgatggc gagttcctca cggcactcgc gaatgacggc ctgtcgaggt gattctcccg
    10021 gctcgacgtg cccgccgacg aggtcccagc agtcgggata ccagcgccgc gcggggtgcc
    10081 ggtgcgccat gaggaccagc ccgtcgcgca ccaggagcgc agtggcgatg gcagttcggg
    10141 cgggcacggg gacaggatag gtgcggcatt ggccgaggcc ggtatggctg tgtcgtacgc
    10201 gagagacggc gcggcgcggt cgtatctaaa ctcatggtgt ggcaacgacg ttgaccgtcc
    10261 ttgggtcgtg tggtgcgtgg tcggaggcgg gcagggcctg cagcggcgtc ctgctcgatg
    10321 ccgacgcatt ccgcgttgtc atcgacctcg ggtatggcac tgcctctcgg ctcttccacg
    10381 cactgggctc gacgtccggc gacgggttgg atgctgtcgt catcacgcac agacatcccg
    10441 accacatggt ggatctgcat gccttatttc gtgcccggtg gttcgcttct cggaattccc
    10501 cgccgtagcc ggtctatgca gccgtcgggg tgcgcgagca gctggtgtcg atggacgagc
    10561 ctgacgacgg cgccaatgtg gaccgcatct tcgcgttcaa cccactgccg ggcaactaca
    10621 cggtcggccc cttgaccctc tcggcgctcg atttgccgga ccatgtgccg aattcaggtg
    10681 tgcgcatcgc cttccgcgat ctcaccatcg cctacaccgg cgatgccggc ccgtcggagg
    10741 cactggcgga gttggcagca gggggccaac ctcctgctgg ccgaggcgag cgaccgccac
    10801 cagcaacccg gggtcccgcc ctcaccgatt tcggggtatc acctgagtgg ggcagatgcc
    10861 ggacggttcg cagccggagc cggtgtggga agccttgtcc tgacccactt ttggccaggc
    10921 aacgaccgcg aggcaacacg cgccgccgcc gccgcgcact tcgccggacc cattgcgctc
    10981 gccgaggagg gcatggtcat ctcgcttggc tagccaatct cccgaataca tgcatgcctc
    11041 tgatcgcgaa cggcgataac cgtttccgtt gacctgcgat cgttcattcg gctgaattgt
    11101 cggcatgcgg actttccccg acggcttcct cttcggtgtc tcaacggcag gccatcagat
    11161 cgagggcaac aacaccacga gcgacacgtg gttcctcgag cagcaacagc cgagcgtctt
    11221 caccgagccg agcgggccgg cctgcaacag cttcgagctg tgggagagcg atctggacct
    11281 ggttgcctcg ttcggcctga acgcctatcg attctccgtc gagtgggccc gtgtcgagcc
    11341 ggaggagggg cgtttcgacg acgcggcaat cgaccactac gaagcgatgg tcgacgggtg
    11401 cgtagcccgc gggttgaagc ccgtcgtcac gctcaaccac ttcacctcac cgcactggtt
    11461 cgcctgccga gccggctggc tcgatcccga ggcgcccgag ctgttcgccc gctatgtcgc
    11521 caaggtgatc gcccgcttcg gtgagcggct cgccttcgtc atcaccttca acgagcccga
    11581 cctgccggcg atgctgcagt gggccaacct gccgccggtc gtcgccgagt tggaacgagc
    11641 caccctggac gcggcgtcgg cgtccgccgg cgtcgaacgc tatcgcgcgg cgaatgtcgt
    11701 tctccccgaa gacatgcacg cgatcgcgga cggtatgacg gccggtcacc gcgccgcgaa
    11761 ggcgatcgtc aaagaccgct accccgaact gcccgtaggg ttttcgctcg cgatggtgga
    11821 cgacgtcgtc gtcggtgacg acccgagcgt gcgcgatcgg aaacgcgccg aagtctatgg
    11881 ccgctggctc gagctagctc gcgaggacga cttcctcgga gtccagaact acgagcgact
    11941 gacgtatgac ggcaatgggc tggtcctgcc tgagggagct gcgtccggtg cgttgtatgg
    12001 cggcagcgag ccccggtctc tcgcggagtg cgtgaagtac gcgaactccg ttgcgggagt
    12061 gccgatcctg gtcaccgagc acggcatgga agggcaggat gacgccaagc gggtcgcatt
    12121 catcgaggac tccctgctgc atctgctcga cgtgatcgac tcgggcgttg cggttctcgg
    12181 ctacttgcac tggaccctcc tcgacaattt cgagtggatc ttcggctacg agaagacgct
    12241 cgggctggtc gccgtcgacc gggagacgtt cgcgcggacg cccaagccga gcgcggccgc
    12301 gcttgccgcg atcgcgactg gtcgcggcgt ccccgatccc gtcacctccg aggccctcgt
    12361 cgcgaccgat cgacccgggc gctacggcaa gcaactcgcg tcccacttga gcaaacgcgt
    12421 tgcgtcgcaa tgggatgacg agaccggaac gggttcgctc gtcttcggcc acggtggccg
    12481 ggccgacctg gccgcggtcg acggcggctt gcgcttcgag ctcgtcgcgc ggccggccag
    12541 cgtggcccgg ctcgaggatg tcatcggccg ccacctcgtg cgtttcggcg cgcgcgacga
    12601 actcgccgtc gcctggaccc gatccgacgg cactgcgggc accacccaac gcaactccga
    12661 agacgactga gccgcaccgg catacggaac caatccgcat acgaccaagg aagcgaagga
    12721 acccacatgg ctgctggaga tcgcccccgc cgggtcgtca ccgggcatac ccccgacggc
    12781 gtgtcggtcg tcgtgtccga cggcccggtg ccggtcagcc gcgcgctgcc cgacgacggc
    12841 gtcttcttcc acgagatctg gaacactcag gacgcgccgg cgccggtgcg ggccgtcgag
    12901 ccggtcgaac cgaccgaggc cgacctcacc gtcccgccgc cgccgcgcgg cacgaagatc
    12961 cggatcaacg agttcctccc cggccacctc gacgagcgag gcctgcagtc tccggtgcac
    13021 cggacccagt cgatcgacta cggcatcgtt ctcgaaggcg agatcaccct gatcctcgac
    13081 gacaccgagg tcacgctgcg ggccggcgat atcgtcgtcc agcgcggcac ggaccacgcc
    13141 tgggccaacc gctcggacgg catcaccaag gtcgccttca tcctcgtcga cggtcagttc
    13201 gatcccgttc tcgccgagtc catcggtggc ctggacggtc tcatgcacgg cggcccccgc
    13261 gattaggtaa gacacctcgg cggttatacc cgcccaaccg tcttacctac cggtgggtac
    13321 tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtagagg tcgatgacgt
    13441 cggtgcgcag ccgggtcagg gcttcgtcgc acaggcgccg aatcgactcc ggcgcaccgt
    13501 cgatgacgcg tttgccgtcc cggccgtaca tgccgcattt gctcgccaat gtgatgtcgg
    13561 cgcggcgaga gccgagagtg gcgccgagca ggtcctcgtt ggcgccgaac ccatagagcg
    13621 cggcggtgtc gaagtggtcg acgcccgcgt cgagggcggc cgcgatcagg gcagcaccct
    13681 gttccggcga ggggatcggg tgatagacgt ggctgaggtt catgcacccc aggccgatcg
    13741 gggcaacctc gaatggcccc agccggcggc ggggtgcgtc ggcgagcgcc atcagccgtg
    13801 ggcctcccgc tgcgggtcga cttgttgctc gagatcgccg agcacccggt agcagtcgat
    13861 gacctgggcg atcgaggaat tcggctcgcg gccctcgcga atcgcggcga cgaactcccg
    13921 gtcctgtaac tcgataccgt ccatcgacac gtcgaccttg ctgacgtcga tcgcctcgtc
    13981 cttgccgtcg gtgaggtcgt cgtagcgggc gatataggtg cccgtgtcgc cgatgtagcg
    14041 gaagaaggtg ccgagcgggc cgtcgttgtt gaacgacaac gacagcgtgc agatcgcgcc
    14101 gttctcggcc ttgagctgga tggacatgtc catggcgatc cccaagtccg gatggctggg
    14161 cccggcgatg gcgtgggctt gcacgatggg cgaacccgcc tggtacgcaa acagatcgac
    14221 cgtatgagcc gcgtggtgcc agagcaggtg atcggtccag gaccgcggct ggccgagggc
    14281 gttgaggttc ttgcgccgga agaagtaggt ctgcacgtcc atctgctgga tgtggaactc
    14341 gccggccgcg atcttgttgt gcacccactg gtggctggga ttgaaccggc gcgtgtggcc
    14401 gaccatcccc acgagtccgc tctcctgctg gacgcgtagg cactcctcgg catcggggag
    14461 ggagtcggcc agcgggatct cgacctgcac gtgcttgccg gcccgcaggc acgccagtgt
    14521 ctgtgaggcg tgcatgggcg tcggagtcgc gaggatcacc gcgtcgatgt cgtccatcgc
    14581 gagcacatcg tcgaggctct cgacagcgcg accgacaccg tgctcggcgg cgaacccctg
    14641 ggccgactcc acagagccgc tgatgatggc agccagctcg acgccgtcga tcttcttcag
    14701 gccgttgacg tgcttggcgc cgaaggcgcc gccaccggcg agggcgatgc gcaagggttg
    14761 gtcggtcatc tcactccttc gccgcagcgg cagccggcgg cttgttgttg aggatcaggt
    14821 gtcccacagc agtattggac gcaggcacat ggtagaagcg gtggacgacc tcgatctcgc
    14881 cgtcgccctc gacgtcggac atggcgccgc gggcgatgaa ccacatcacg agctcgatgc
    14941 cctcggagcc ggcctcctcg acatactcca ggtgcggcac gtgcgagaga ccttcggggt
    15001 cctcgatgag ccggtcgagg aaggcgttgt cccactcgcg gttgatgaga ccggcgcgcg
    15061 ggccctgcag ttggtgactc atgccgccgg tgccccagat ctgcaccttg atcggcttct
    15121 cgtaggactc gatggccttg cgcagcgcct tgccgagctt gaaacaacgc tcaccggacg
    15181 gcaccggata ctgaaccacg ttgacgtgga agggaatcac cttcaccggc cacgtgtcga
    15241 cctggccgaa catcagcgac atcggcacgg tcaggccgtg gtcgacctcc atctcgttga
    15301 cgagcgtgag gtcgaagtcg tcacggatga gcgagtgggc tacgtgcgcg gcgaagtccg
    15361 ggtcaccctc gatgcccggc accgggcgcg ggccgtagcc ctcgtcggcc gcggggtagg
    15421 cagcgccggt gccgaggacg aaggtcggga tcagcgacag gtcgaacgcg gttgcgtggt
    15481 cgttgtagac gaggaagacg acgtccggga ggttgtcctg ctcccaggcc ttggagaact
    15541 catagccgtc gaaaacggcc ttccagtaag gctcttcggt cttgccgagg tcgagtgcgg
    15601 cgccgatcgc cggcacgtgc gaggtgaaca cggcgccggt gatcgtcgca ttgcccacgc
    15661 tgtcgtcggc cgcggcgtcg gcgcgcacgg cgtcgagcat cgggtcctgc tcctgggcag
    15721 ccgcaatctc tgcctcggtg cgctcgacct ccgggacggc gacgtcggcg atccggttgc
    15781 ccgcgatgga gcgcccaccg ccgaccatca tgtcgcggta ttcctgctcg gtcatgccgg
    15841 tcatggagcc ggccatctgc tggaacgaca ggcccaaggt ggcgccgaac ttcgccagga
    15901 agtagatatt gccgccctcg cgcatcgctg cgttcaggtc gcggtcgcgc agcgccttct
    15961 tctgcgcgtc cgtcagcggc cactcgtcca tgtaggcgtc ctcgtcggca aggaaccgct
    16021 cccggttctc cgccttcatc agcgacatac agaactggtt gaggtggtag cccttggccg
    16081 actgctccga gtcgaagatc gtcgtgccgg ggaccttgcg atactccttg tccagagcca
    16141 tggttcagcc ttcctcgggc cagtagaggc gcatcgggtt gtcgacgagg agcttgcgct
    16201 gggactcctc ggcggtggcg atcgagggga tccaatcgac gagcagcccg tcgtcgggca
    16261 tgtggtcttt cagattaggg tgcggccagt cggtgcccca caacacgcgg tcggggaatt
    16321 cctcgacgac ccgcttggcg aagggcacga cgtcacggta tgccgcctgc tcgcccccga
    16381 gtgcccgcgg gccggtcgtg gtgagccgct cggggcaggt gaccttgcac cagatgtcgt
    16441 cgcgggcgcg catcaggtcg aggaagccgg tgaaccccgg accgtcgacg tccgtggtga
    16501 cgtcggggcg gcccaggtgg tcgacgacga gcggcacggg caggccgagg aagaagtcgg
    16561 cgagttcggg caactcctgg gactcgaaat agatgacgat gtgccagccg agcgggccga
    16621 tctgatcggc gaccttctgc agcccgtcca tcggcaggac gtcgaccagt cgcttgacga
    16681 agttgaagcg gacaccgcgg acgccggcgt cgtgcaggcg ttgcagttcg tcctgcgcca
    16741 ccccggggcg caccgtcgcg accccgcggg ccttgccccc cgacgactgg atcgcgtcga
    16801 ccatcgcgct gttgtcggcg ccgtggcagg tggcctgcac gatgacgtta cgggcgaagc
    16861 cgaggtggtc gcgcagcgca tacagctgct ccttggaggc atcgcacggg gtgtatttgc
    16921 gttccggcgc aaagggaaac tcctcccctg ggccgaacac gtgacagtgc gcgtcgacgg
    16981 cgcccgccgg cagccggaag cggggagtgg acgggccggc ataccaatca agccagcccg
    17041 gagtcttctc gaactcggtc ttctggggcg tgctcacgcg ggccattcct tgtaggtcag
    17101 tccggctttt tccagcggtt cgcgcatgtt gtagaggtcc aacccgagca cgccctcacg
    17161 gaacttcgcc cgcttgctct cctcgttgtc ctcgcgcgtc ttggcggcct cggcgatctc
    17221 cgccgcccgg tcgcggggaa ccacgacgac gccgtcgacg tcggcgacga cgacgtcgcc
    17281 ggggttgacg atcgcgttgg cgcagacgat cgggatattg accgatccga gcgtcgcctt
    17341 gaccgtgccc ttggaattga tcgcgcggga gaagacgggg aagttcatct tctccaggtc
    17401 agcgacatcg cgcacgccgc cgtcgatgat caggccgccg cactcgcggg cgcgcaggct
    17461 ggaggcgagc agctcgccga agaacccgtc ctccgattcg gtcgtgcagg cggcgaccag
    17521 gacgtcgccc gggccgacct gctcggtcgc gacgtggaac atccagttgt cgcccggctg
    17581 cagcagcacg gtcaccgccg gcccacagat ccgcgcgccg gggaaggcgg ggcggatata
    17641 ggggcgcatc aggccggtgc ggcccattgc ctcgtggatc gtggccacgc cgaaggcgga
    17701 caacgcctcg acggcggccg catcgggacg gggcgtgccg gtgtagacca caccgagttc
    17761 gttcggattc accgttgatt cctttctggc cgttgcttga ccaccttctc ggtcccacgg
    17821 cacgtggcgt aacggggatt ccgccaggcg gaagcgctgc cagacggcgg cgcggtagag
    17881 gcgaggtgcg gggcactgtg tgcgcgatgg cctagcatgt ccgggtccga actctcggac
    17941 ctgtcgatgg cccggcgccg ccgctgaaag gcgcctcacg atgtgtgaat tccactccgc
    18001 caccatcgtc cacgccttca cggccgcctg ggaataccag tgctgtggta ctcccgtcag
    18061 cgtcggtgac gaactcaccc ttcggcacgt cgcctgggat gagggccttc ccggcaacga
    18121 cgcctaccgc cgggtgcaac acctcgattg gtatgtcggg caccacgtca cctctgaaga
    18181 ggaaggggct gccgaggatc ttgtccgcgt gctcgcgctg tgggaggccc attccgacct
    18241 cgacttccgc gccggggacg acggctacta cgcagcggcg cgcgacagcg gccggctcgt
    18301 gccgatcacg acgatggtgc ctttcgacgc gacgtgggcc ggtgacggtc caccgacgcc
    18361 gcccgcacga cacaccccgc gagaacccca aggctggatc atgaagttgg aggtcctccg
    18421 ccgcgacgga tgacccaccc ggctttcagc gcccccgcgc cgtcaaggtg gcgtcgaggc
    18481 ggggataggc acgacgcgcg ttcgcctcat acacggcggt gcgctgctca tcggtgagat
    18541 tcggcgtcgc gtcgacgtag cgcttggtgt cgtcgaagta gtgcccggtg cgcgagtcga
    18601 tgccgcggac ggcgccgatc atctcgctgg cgaagaggat cgactcgctc gggatgacgc
    18661 tcgtcagcag gtcgatgccg ggctggtgat agacgcaggt gtcgaagaag acgttgcgca
    18721 gcaacgct
//
LOCUS       DAOPJG010000042        18704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3109_length_18704_cov_7.414607, whole genome shotgun sequence.
ACCESSION   DAOPJG010000042 DAOPJG010000000
VERSION     DAOPJG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 18704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18704
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3109_length_18704_cov_7.414607"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PKU75_05825"
     CDS             complement(<1..471)
                     /locus_tag="PKU75_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775346.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPF80432.1"
                     /translation="MSQMYYDVRTHGCQMNVHDSERLSGLLESAGYVDIESLPVGERP
                     EVADVVVFNTCAVRENADNKLYGNLGTLRQAKLKKPDLQIAVGGCLAQKDRGTIVERA
                     PWVDVVFGTHNIGSLPALLDRARHNKQAEVEILEALEVFPSTLPTRRDSAYAGWV"
     gene            540..1301
                     /locus_tag="PKU75_05830"
     CDS             540..1301
                     /locus_tag="PKU75_05830"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HPF80433.1"
                     /translation="MTTIDLNADLGEGFGRWTLTDDAGLLDVITSANVACGYHAGDAR
                     TMDRVCRIAAERGVAIGAQVSYRDLAGFGRRFIDTEPADLEADVLYQVGALEAFARAA
                     GTRVAYVKPHGALYNAVVHHEAQAAAVVAAVQRYDGQLDLLGLPGSRLLALAGEAGLR
                     AVAEGFVDRAYTADGTLVPRNESGAIVAEAGRPARAVRLAMDQRVDTVAGGEIPMRVE
                     SLCVHGDSPDAVATARDVRAALESAGAAVRAFVAE"
     gene            1303..1920
                     /gene="pxpB"
                     /locus_tag="PKU75_05835"
     CDS             1303..1920
                     /gene="pxpB"
                     /locus_tag="PKU75_05835"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HPF80434.1"
                     /translation="MEILPCGAEALLVECDSLVDALALDAVMIPTALPGVIETVPAAR
                     SVLIHFDPTRTSADFVAAQVRRLSTTATGVEPASRVVEIDLAYDGPDLAAVAQLLGVD
                     REEVIARHTATEWRCGFVGFAPGFPYLVGGSDLLRVPRHAEPRVSVPAGSVALAGEYC
                     GIYPRTSPGGWQLIGTTRAPLWDLTRTEPALITPGARVRFVPVQT"
     gene            1917..2774
                     /locus_tag="PKU75_05840"
     CDS             1917..2774
                     /locus_tag="PKU75_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HPF80435.1"
                     /translation="MSRGFRVVEPGLLASVQDLGRPGYARLGVSRGGAADRGALRLAN
                     RLVGNPEDAAGIEVTAGGFSGYAESPLLVAVTGALAPVHVDDRPASTNAPLSLAAGAR
                     LAIGRPTAGLRNYVAIAGGVTSEITLRSRSYSRLGGLGAALAPDDLVPIGTGASASPV
                     VDHAAIPSPPVDATTLAALPGPRRHWFSDEAVAQLMATTYRVTAELDRTGIRLDGPAV
                     PRCREEELPSEGMVRGAVQIPASGRPIIFLSDHPATGGYPVPLVLTPDAADHAAQLTP
                     GMTVRFALE"
     gene            2826..3536
                     /locus_tag="PKU75_05845"
     CDS             2826..3536
                     /locus_tag="PKU75_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80436.1"
                     /translation="MSDSTSIEDILGPRPEITRGHATLECAYPAGEETFTGLMMLRIA
                     TGAYEDTDELVEGFVEMAEEFEEYFPDDEPVAPTDVPARFQQVLDEYKRVVHTTSYDA
                     YCLLSLPEILPSAGLILSWGDGFDSSEALEVVRDLADQYAEQGIDMRGYVYAHTQDLE
                     RLVLTGDLYLGFGVLGPRDDVAAVAVGRQVVDVLRQLGLPFTWDGTVGARILVAPVLY
                     EIELEDETGEDGIGEDAD"
     gene            3569..5674
                     /locus_tag="PKU75_05850"
     CDS             3569..5674
                     /locus_tag="PKU75_05850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPF80437.1"
                     /translation="MTNPALRERAVAALRQLVGRPDAEFRDGQYDAIAALVEGRGRVL
                     VVQRTGWGKSAVYFVATTLRRAEGAGPAVIISPLLALMRDQIAAAERAGIRAVTMNSA
                     NATDWDQVRSALTADEVDVLLVSPERLNNPRFREEQLPDLARRCGLLVVDEAHCISDW
                     GHDFRPDYRRIKDLLEGLPAGTPVLATTATANARVVADVETQLAAGGAPVQTIRGGLA
                     RESLRLGVLRSMSPEQRLGWLIAHLDDLPGSGIVYTLTVSGAEDIAAALSEAGHRVAA
                     YTGRTDHADRERLEMSLRDNAVKALVATSALGMGFDKPDLGFVVHVGAPASPVAYYQQ
                     VGRAGRATERADVLLLPGPEDQDIWRYFASVSMPREEQADAVLRALAEAGRPLSTAAL
                     ETIVDIRRTRLELLLKVLDVDGAVTRLSGGWTSTGVPWTYDGDRYAWVAAQRQAEQEQ
                     MLAYERGSECRMAFLQRALDDPSAADCRRCDNCAGVWFPTDIPDRVIGAARERLDRVG
                     VVIEPRAMWPTGMDRLGVPVKGKIAPAEQASEGRALARLSDLGWGQRLRPLLQHDGPV
                     PPEVLRGVVEVLKGWGWARRPVAVMSVPSRARPQLVGSVAAGIAEIGRLTYLGELALV
                     DGGPTGEAGGNSAFRLAGVWERLAIGADLAASIRSLDGPILLVDDYVNSRWTVTEAAR
                     ALRVSGADEVLPLVLAIDA"
     gene            5979..6380
                     /locus_tag="PKU75_05855"
     CDS             5979..6380
                     /locus_tag="PKU75_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80438.1"
                     /translation="MQRTETRTTYTPGATAGEERKGFNPLWLLLPLLAALALWLGWRA
                     MDNDDEPADTTTTSAAATGTEITVPSFDEMTTKLTDAGVANAQSVAQGIENAGPYTAD
                     NLVEKITTAVSSAGVDATDVTKTIEALGLPS"
     assembly_gap    6407..6506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6554..7255)
                     /locus_tag="PKU75_05860"
     CDS             complement(6554..7255)
                     /locus_tag="PKU75_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80439.1"
                     /translation="MSPIHLARRGLARRVELVPMSPVQQLRAGRITHRLTQLLVGLTL
                     YGVSMAMLLRSTLGLDPWDVFHQGVQGHWGVSFGTVVIVTSFVVLLLWIPLRQWPGLG
                     TILNSIVIGLATDATLRILDAPTGLLGRSALLLGGIVANGLAGALYIGSQFGPGPRDG
                     LMTGLARRTGRSLRLVRTSLELTVLLLGWLLGGTVGIGTVVYALLIGPIVQFFLPICT
                     VRLRQPQPTVVTGSL"
     gene            7318..8763
                     /locus_tag="PKU75_05865"
     CDS             7318..8763
                     /locus_tag="PKU75_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPF80440.1"
                     /translation="MTPAVSAPRLARLVGDGALARPAYRSLAAAIMTLLADGRLVSGT
                     RLPSERELTGALGCSRTTVTSAYAVLAERGYLETRRGSGSIAVVPGRLAAASRVLSPA
                     TDLAPDAIDLTCAATRAPAGILEAYAAALADLPSYLGDNGYAPVGLLDLRAVIAERFT
                     RRGLATTPEQIIVTSGAVAGLAVISRALLTRGQRVLLESPSYPNAMAALAMEGARTVA
                     IPVDAAGWDLDEAARIVPASGASAAYLIPDFHNPTGALLDDSGRERLAGLLAAADVRP
                     IIDETIADIDLDGGPPVRPFGCFSPGAIVLGSAAKVFWGGLRIGWIRVPTALASRVVS
                     ARATLDLGAPILEQLVLMHLLVTHDGLPAARRTELLAARDATVEAVGTHLPHARFVVP
                     RGGLTLWVDMGDLPVGGLVLAAAEAGLLVAAGPQFAPVGGFERFIRIPYVAAPAVMTD
                     AVARLARAAEELRAGGVTALTHPPRAGGFVA"
     gene            8799..9179
                     /locus_tag="PKU75_05870"
     CDS             8799..9179
                     /locus_tag="PKU75_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HPF80441.1"
                     /translation="MSDVVFERVHHVQVAIPVGGEDSARAFWVELLGFAEVPKPPVMA
                     ARGGCWFRAPGGGLEMHMGVEQPFAPARKAHPGILIAGLDALATRLEDAGYAVRWSDE
                     IPGTKRFHTDDPFGNRLEFIDAGA"
     gene            complement(9209..9940)
                     /locus_tag="PKU75_05875"
     CDS             complement(9209..9940)
                     /locus_tag="PKU75_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF80442.1"
                     /translation="MSDHPPFAVTVDLAVFTIHEGTLAVLLVRRGEDPFAGHWALPGG
                     FVEIDEDAATAAWRELAEETGLERFSGHLEQLATYSRPDRDPRMRVVSVAHVAFAPGL
                     ARHVPTAGSDAAAARWWPVEDLGLDGADETSVELAFDHAEILRDALERVRSKIEYTTL
                     AAQFVEEPFTLADLRRVYAAVWGAAPDLGNFRRKVLSTPGFVVATEDRDSGDGRSGRP
                     ALLYQRGPATALQPAMLRPDDTGAG"
     gene            complement(9937..10515)
                     /locus_tag="PKU75_05880"
     CDS             complement(9937..10515)
                     /locus_tag="PKU75_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPF80443.1"
                     /translation="MSSTALLVVDVQNDFVEGGSLAVTGGRAVAAAIVDHIDRYAGHY
                     RLIVGSRDWHSPETDNGGHFATAGDPDFAATWPAHCVAGTPGADYAEPRLAERLTHHV
                     RKGMGAPAYSMFEGITDDHRTLADLLAAEGMDEVDVVGIATDHCVRATALDAARAGLR
                     TTVLLPLTAGVAPESTEAALTELVATGVEVAR"
     gene            complement(10518..11843)
                     /locus_tag="PKU75_05885"
     CDS             complement(10518..11843)
                     /locus_tag="PKU75_05885"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPF80444.1"
                     /translation="MAKTQAPRSTAARQPAGASPGMLTDRYELTMYSSFVEDGSVDNH
                     SVFEAFARRLPPGRRYGLLAGLGRLIPMIAGFGFTDDELAFLQATGAITTRAADRLAN
                     FRFSGDITAYPEGETYVPGSPVLTAEGPLGECLLLETLVLSVLNHDIAVASAAARMVT
                     AARGRPIIEMGSRRTHEQAAIAAARAAYLAGFDTTSNLAAGHLHGIPAVGTVAHAFIL
                     AHDSERDAFASQVAALGAGTTLLVDTFDIEQGIRTAVEVAGPELGGIRIDSGDLDVES
                     RRARTLLDELGATKTRIVVTSDLDEYVITALGAAPIDGFGVGTRVVTGSGHPTAGMVY
                     KLVAVSDRPGAEAPLRPVAKKASGKGSAGGRKTAYRLPDGSEHFRLDGVIPDGGRPVQ
                     VPVIRGGEMVHEPSLAEVRAHAAAALAALPPSAHEVADGEPYTTAMEEE"
     gene            complement(11945..12613)
                     /locus_tag="PKU75_05890"
     CDS             complement(11945..12613)
                     /locus_tag="PKU75_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPF80445.1"
                     /translation="MTDHTPADLSALRAAIGRIEAGGMPHHSPPGSSPDATPSGDDPT
                     QPARDDEEPHPASRGEAARDPHAVAKAIVLRQLTNSPKTRAELEEALAKRDCDPLVAK
                     DVLDRLTEVGLVDDAAYAQMFVRSKQRRSGLAASALRRELRAKGVDPDTAGAAVDDVS
                     PALEEERARELVRSRLKRLHGLDRAVQVRRLAGLLARKGYPSGMASKIIFDEIDQTAE
                     HARD"
     gene            complement(12613..13110)
                     /locus_tag="PKU75_05895"
     CDS             complement(12613..13110)
                     /locus_tag="PKU75_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80446.1"
                     /translation="MRKFDIEVGGTHNYFVDGVMVHNSPETTTGGKALKFYASVRLDV
                     RRIETLKDGNEPVGNRTRVKVVKNKVSPPFKQAEFDILYGMGISREGGLIDMGVDQGF
                     VRKSGAWYTYDGDQLGQGKENARTFLKDNPDLADELEKRIKEKLGVGPRVDAPAEGAE
                     VPVEF"
     gene            complement(13282..13836)
                     /locus_tag="PKU75_05900"
                     /pseudo
     CDS             complement(13282..13836)
                     /locus_tag="PKU75_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015141.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG endonuclease"
     gene            complement(13897..14769)
                     /gene="recA"
                     /locus_tag="PKU75_05905"
                     /pseudo
     CDS             complement(13897..14769)
                     /gene="recA"
                     /locus_tag="PKU75_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779722.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
     gene            15065..15547
                     /locus_tag="PKU75_05910"
     CDS             15065..15547
                     /locus_tag="PKU75_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF80447.1"
                     /translation="MRNGQPFEAAVQEHGPRVLRVCRAVLGPGHDADDAWSETFLAAL
                     RAWPTLDRGTNIEAWLVRVAQRKCIDVIRAKSRRAIPSDVLPEPDSPPPPADKSIWYA
                     VARLPEKQRLALAYHYYGGLPHTETAELIGSTPAAVRRAASDGIASLRRILAENGEIV
                     "
     gene            15544..16173
                     /locus_tag="PKU75_05915"
     CDS             15544..16173
                     /locus_tag="PKU75_05915"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPF80448.1"
                     /translation="MSEQNPGLADPLPSLDPASPIDVADLTALHARLIAGAKTEGLID
                     IAYRTVDSPVGPLLVAATSTGLVRVAFEIEDFDRVLAALAALVSPRILHTPHRLDIVA
                     RALDDYFAGRRTGFDLPLDHALSSGFRERVQRHLVQIPYGQTTTYGQLAADLGSPRAV
                     RAVGTACSTNPLPIIQPCHRVLRGDGALGGYLGGAHVKRSLIALEAGNR"
     gene            16482..18587
                     /locus_tag="PKU75_05920"
     CDS             16482..18587
                     /locus_tag="PKU75_05920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPF80449.1"
                     /translation="MTSVTTAAPGSTTARASGFRPDVEGLRAIAVGTVLWFHAGLGGL
                     RGGFAGVDIFFVISGFLITGQLVREVERSSRISLPRFYARRAKRLFPAAATVLLATAV
                     LTVLFLPKISWRVFGWDIAASAAYVVNWRLADRAVDYLAEDTSVSPVQHFWSLAVEEQ
                     YYIVWPLLLLALAVLIRRARLPVRPTMAIGLAAIVLPSLAWSIHLTEADPARAYFVTT
                     TRLWELGIGALVAVGATRWRRISAPLGAALAWSGLAVIAVGLVAQSTATPWPGWHALV
                     PVAGTAAVILGGHSAGRLGPVRVLGTRPMVWIGGLSYSLYLWHFPLIVAASAHLGELT
                     RWQGSAVAVASVMPAWLTHRFIENPLRFHPRVAEHTRNALVLGLTCSLLGVGAGYAVT
                     RVGASPTLDAAQVAQLRAAHQFGGQVLGSGQQPSSAGIDRHPQAMTPKPADAPADAPV
                     LYADGCQTVRTTTEVVTCVYGVKGARKVMALVGDSKAAQWFPALERYALERGWEIRTY
                     LKSACPWSPALIFDGDNPTRRYPNCQQWGKDVLARLLGPEKPTVVVTSGIRSVAFGAD
                     GTERESVMVDGYVDYWTQLGRAGVPVLALMDTPQPGRIVYECVLEHKDDYMTACQGAW
                     NDGSGGPALRAATDRVPTSRLVDLNPWVCPDRTCWPVIGSVLVWRQGSHLTATYTESL
                     ASVVAREISAALTQVGVQP"
BASE COUNT         2636 a         6485 c         6577 g         2906 t
ORIGIN      
        1 cacccagccg gcatacgcac tgtcgcggcg cgtcggcagc gtcgagggga acacctcgag
       61 agcttcgagg atctccacct cggcctgctt gttgtgccgg gcccggtcca gcagggccgg
      121 cagcgagccg atgttgtggg tgccgaagac gacatcgacc catggcgcac gttcgacgat
      181 ggtgccgcgg tccttctgcg cgaggcagcc gccgacggcg atctgcaggt ccggcttctt
      241 cagcttcgcc tgacgcaggg tgccgagatt gccgtagagc ttgttgtccg cgttctcccg
      301 gaccgcgcac gtgttgaaga cgacgacgtc ggcgacctcg ggccgctccc ccaccggcag
      361 cgactcgatg tcgacatagc ccgccgactc gagcaggccg gacaggcgct cggagtcgtg
      421 gacgttcatc tggcacccgt gggtgcggac gtcgtaatac atctggctca tagcaccgtc
      481 aagggtacgt gctcgcgggc gaccggccgt tcccgttcga gtgcggcgac aatggtgccg
      541 tgaccacgat cgatctcaac gccgacctcg gggagggctt cgggcgctgg acgctgacgg
      601 acgacgccgg cctgctcgac gtcatcacga gcgccaatgt cgcgtgcggc taccacgccg
      661 gggatgcgcg gacgatggat cgggtctgcc gcatcgccgc cgagcgtggc gtggccatcg
      721 gcgcccaggt ctcctatcgc gatctcgcag gattcgggcg gcggttcatc gacaccgagc
      781 ccgccgacct ggaggccgat gtgctctacc aggtcggggc gctggaggcc ttcgcccgtg
      841 ccgccggcac ccgggtcgcc tacgtcaaac cccacggggc gctgtacaac gcggtcgtgc
      901 accacgaggc gcaggccgct gcggtggtcg ccgccgtgca gcggtatgac ggtcagctcg
      961 acctcctggg gctgcccgga tctcgcctgc tcgccctggc gggtgaggcc gggttgcgtg
     1021 ctgtcgcaga gggtttcgtc gaccgggctt acaccgcaga cggaaccctg gtgccgcgca
     1081 acgaatccgg cgccatcgtc gccgaggccg ggcgtccggc acgggcggtg cgcctggcga
     1141 tggaccagcg ggtcgacacc gtcgccgggg gcgagatccc gatgagggtg gaaagcctgt
     1201 gcgtccacgg cgactcaccg gacgcggtcg ccacggcccg cgacgtccgt gccgcgctcg
     1261 aatccgctgg ggccgccgtt cgcgccttcg tcgccgagtg acatggagat cctgccctgc
     1321 ggcgcagaag cgcttctcgt ggaatgtgat tcgctcgttg acgctctcgc cctcgacgcc
     1381 gtcatgatcc ccaccgcgct cccgggagtg atcgagacgg tcccagccgc ccggagtgtg
     1441 ctgatccact tcgacccaac ccgtaccagc gccgatttcg ttgccgcaca ggtgcgtcgc
     1501 ctctcgacta cggccacggg agtcgagccg gcgagccgag tcgtcgagat cgacctggcc
     1561 tacgacggtc ccgacctcgc cgcggtggcg caactcctcg gcgtggaccg ggaggaggtg
     1621 atcgcgcggc ataccgcgac cgaatggcgt tgcggcttcg tgggtttcgc tccggggttc
     1681 ccctatcttg tcggcggcag cgacctgctg cgggtgccca ggcacgccga gccgcgggtc
     1741 agcgtgccgg ccggatcggt cgcgctcgcc ggcgaatact gcggcatcta tccccgcacc
     1801 tcccccggcg ggtggcaact catcggcacc acacgcgcgc cgctgtggga tctcacgcgc
     1861 accgagccgg cgctcatcac ccccggcgcc cgcgtccgtt tcgtcccggt gcagacatga
     1921 gccgcggctt ccgggtggtc gagccggggc tgctggcgtc ggtgcaggac ctcggtcgcc
     1981 ccggctatgc ccggctcggc gtcagccgcg gcggggccgc cgaccggggg gcgctgcggc
     2041 tggccaatcg cctcgtcggt aaccccgagg atgcggccgg gatcgaggtc acggcgggtg
     2101 gcttcagcgg gtatgccgag tcgcccctcc tcgtcgccgt caccggcgcc ctggctccgg
     2161 tccacgtcga cgaccggccg gccagcacga acgcgcccct gagcctcgcg gccggggcgc
     2221 gcctggcgat cggccggccg acggccggcc tgcgcaacta tgtggcgata gccggcggcg
     2281 tgacgagcga gatcaccttg aggtcacggt catacagccg gctcgggggg ctcggcgccg
     2341 ccctggcacc ggacgacctg gtgccgatcg gcaccggggc gagcgcatcc cccgtggtcg
     2401 accacgcggc catacccagc ccgccggtgg atgcgaccac cttggcggcg ctacctggcc
     2461 cacgccggca ctggttcagc gacgaggctg tggcgcaact gatggcgacg acataccggg
     2521 tgacggccga actggatcgc accggcatac ggctggacgg ccccgccgtg ccgcgttgcc
     2581 gggaggagga actcccgagc gaaggcatgg tccgcggcgc ggtgcagatc ccggcctccg
     2641 ggcgaccgat catcttcctg tccgaccatc ccgccaccgg cggctacccc gtcccgctcg
     2701 tcctcacccc ggacgcagcc gatcacgcgg cccaactcac gcccgggatg accgtgcgct
     2761 tcgccctcga gtgacgacgc cgcctaccaa aggggcgctg acggaccgca tgacaccatg
     2821 acccaatgtc cgattccacc agcatcgagg acatcctcgg accccggccc gagatcaccc
     2881 gcggccacgc aaccctggag tgcgcctacc cggccggcga agagaccttc accgggctga
     2941 tgatgctgcg gatcgcgacc ggcgcctacg aggacaccga cgaactcgtc gagggcttcg
     3001 tggagatggc cgaggagttc gaggagtact tccccgacga cgagccggtc gcgccgacgg
     3061 acgttcccgc ccgcttccag caggtcctgg acgagtacaa gcgggtggta cacacgacct
     3121 cctacgacgc ctactgtctg ctctcgctgc ccgagatcct cccgagcgcc ggcctgatcc
     3181 tcagctgggg cgacgggttc gactcgagcg aggcccttga ggtcgtgcgc gacctggcgg
     3241 accagtacgc cgagcagggc atcgacatgc gggggtatgt ctacgcccac acgcaggacc
     3301 tcgaacggct cgtcctgacc ggtgatctct acctcggatt cggcgtcctg gggccccgtg
     3361 atgatgtggc ggcggtggcg gtggggcggc aggtcgtgga cgtgttgcgg caactggggc
     3421 tgcccttcac gtgggatggc accgtcggag cccggatcct cgtcgcgccg gtgctctacg
     3481 agatcgagtt ggaggacgag accggcgagg acgggatcgg cgaggacgcg gactgagggt
     3541 tgtcggcgcc gcggcatacg gtcggggtat gacgaatcct gctcttcgcg aacgcgcggt
     3601 ggccgcgctg cgccagttgg tcggccggcc cgatgccgag ttccgcgacg ggcagtatga
     3661 cgccatcgcg gcgctggtcg agggccgcgg ccgggtgttg gtggtgcagc gcacgggatg
     3721 gggcaagtcc gccgtctact tcgtcgccac gacgctgcgg cgcgcggagg gcgcggggcc
     3781 ggcggtgatc atctcgccgt tgctcgcgtt gatgcgtgac cagatcgccg ctgccgagcg
     3841 ggcgggcatt cgggcggtga cgatgaactc ggccaatgcc accgactggg accaggtgcg
     3901 gtcggccttg acggcggacg aggtggacgt gctccttgtc tcgcccgagc ggttgaacaa
     3961 tccccgtttc cgcgaggaac agctgcccga cctcgcccgc cgttgcggat tgctcgtcgt
     4021 cgacgaggcg cactgcatct cggattgggg gcacgatttc cgccccgact accgtcggat
     4081 caaggacctg ctcgaggggc tgccggccgg cacgccggtg ctcgcgacga cggcgacggc
     4141 caatgcccgc gtggtcgccg atgtggagac ccagctagcc gccggcgggg cgccggtcca
     4201 gacgatccgc ggtggcttgg cgcgggagtc gttgcggctg ggcgtcctgc gctcgatgtc
     4261 gccggagcaa cggctgggct ggctcatcgc gcacctggac gacctgcccg gcagcgggat
     4321 cgtctatacc ctcaccgtca gcggcgcgga agacatcgcg gcggccttgt ccgaggcggg
     4381 tcaccgggtg gcggcctaca ccggccgcac cgaccatgcc gatcgcgaac ggctggaaat
     4441 gtccttgcgc gacaacgccg tcaaggcact cgtcgcgacc tccgcgctgg gcatggggtt
     4501 cgacaaaccc gacctgggct tcgtcgtgca cgtcggcgcc ccggcctccc cggttgccta
     4561 ctaccagcaa gtcggtcgcg ccggccgggc caccgaacgg gccgatgtgt tgctcctgcc
     4621 cggcccggag gatcaggaca tctggcgcta cttcgcgtcg gtgtccatgc ctcgtgagga
     4681 gcaggccgat gcggtgctgc gagccttggc cgaggccgga cggcccttgt ccaccgcggc
     4741 cctcgagacc atcgtcgaca tccggcgcac ccgcctggaa ctgctgctca aggtgctcga
     4801 cgtcgacggc gccgtcacgc ggctcagcgg cgggtggacc tcgaccggcg tgccgtggac
     4861 ctatgacgga gacagatatg cctgggttgc cgcacagcgg caggcggagc aggagcagat
     4921 gctggcctac gaacgcggca gcgagtgccg gatggccttc ttgcagcggg ccctggacga
     4981 cccgagtgcc gcggactgcc gacgctgtga caactgcgca ggcgtgtggt tccccaccga
     5041 catccctgat cgggtcatcg gggcggcccg cgaacgtttg gaccgggtgg gggtcgtgat
     5101 cgagccgcgg gcgatgtggc cgaccgggat ggaccggctc ggggtgccgg tcaagggcaa
     5161 gatcgcgccg gccgagcagg cgtcggaagg acgggcgctg gcgcggctgt ccgacctggg
     5221 ctggggacag cgtctgcgcc cgctgctgca gcacgacggg ccggtcccgc cggaggtgct
     5281 ccgcggggtc gtcgaggtgc tcaaggggtg ggggtgggcg cggcgaccag tggccgtgat
     5341 gtcggtgccg tcgcgggccc ggccgcagct cgtgggttcc gttgcggcgg ggatcgccga
     5401 gatcgggcgg ctgacctatc tcggagagct ggcgctggtg gacggcgggc cgacaggcga
     5461 ggccggcggc aacagcgcgt tccggctggc gggcgtctgg gagcgtctcg ccatcggcgc
     5521 tgacctcgcc gcgtcgatcc ggagcctgga cggtccgatc ctgctcgtcg atgactatgt
     5581 gaactcccgc tggacggtca ccgaagcggc ccgggcgctg cgggtctcgg gcgcggacga
     5641 ggtgctgccg ctcgttctcg ccatcgacgc gtagctgcgg gacacgagat cgccgacacg
     5701 cggcgattgc ggcaggactg ccgctgattc ggccaatcag tgacaaattg tcagatccct
     5761 taggggacat cgcccccgag actctgggac atttgctcca cccgataggg tgcaccctgc
     5821 tccgccgcag agagctgttt cgccctcttt agacactctt tgaaatctta gagcgaatag
     5881 cccggggtcg accccttacg gtttccaagg cctaatcaag gatcaagacc tacggctacg
     5941 acgatgccga tcgccgcgtc gctgcgcgtg aggtgccggt gcagcgcacc gagacccgca
     6001 ccacctacac ccccggcgcc accgccggcg aggagcgcaa gggcttcaac ccgctgtggc
     6061 tgctcctgcc gctccttgcg gcgctcgccc tgtggctcgg ctggcgcgcc atggacaatg
     6121 acgacgagcc cgcggacacc acgaccacca gcgccgcggc gacgggcacc gagatcaccg
     6181 tgccgtcttt cgatgagatg accaccaagc tgacggacgc gggcgtcgcg aacgcccaga
     6241 gcgttgccca ggggatcgag aacgccggcc cctacaccgc cgacaacctg gtcgagaaga
     6301 tcacgaccgc cgtctcttcg gccggtgtgg atgccactga tgtcaccaag accatcgagg
     6361 ctctcggcct cccgagctga atttcagcat ccggctaatc acagcgnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnccat cgaggctctc ggcctcccga gctgaatttc
     6541 agcatccggc taatcacagc gaaccagtta cgaccgttgg ctgaggttgg cgaagccgca
     6601 ccgtacatat cggaaggaag aactgcacga ttggcccgat gagcagtgca taaaccactg
     6661 tgccaatgcc gactgtcccg ccgagcaacc agccgagcaa taggacggtc agttccaggc
     6721 tggtgcgcac caggcgcagc gagcggccgg tgcgccgggc cagcccggtc atcagaccat
     6781 cgcggggccc tggtccgaac tggctgccga tgtagagcgc gcccgccaag ccattggcga
     6841 cgatgccgcc gagcagcagc gccgaacggc ccagaaggcc ggtcggcgcg tccaggatgc
     6901 gcaaggtcgc atccgtggcc aggccgatga cgatggagtt gaggatcgtc ccgaggccgg
     6961 gccactggcg gaggggtatc cacagcagca ggaccacgaa gctcgtcacg atgacgacgg
     7021 tcccgaaact cacgccccag tggccttgca caccctggtg gaagacgtcc cacgggtcga
     7081 gtccgagagt gctccgcagc agcatcgcca tcgagacgcc gtagagggtc aggccgacga
     7141 ggagctgggt caggcggtgg gtgatccgcc cggcacgcaa ctgctgcacc ggagacatcg
     7201 gcacgagctc cacgcggcgg gcgaggccgc ggcgggcgag gtggatcggc gacatgatca
     7261 cgagtgtggc gcgaagtgga ctgtctttct atagccactt gtgcgacagt ggcccgtatg
     7321 acgcccgccg tgtcggcccc ccggctcgcc cgcctcgtcg gcgacggagc gctcgcgcga
     7381 ccggcatacc gctccctcgc cgccgcgatc atgaccctgc tggccgacgg ccggctcgtc
     7441 tcgggcaccc ggctgccgag tgagcgcgag ctgaccgggg cgctcgggtg ctcgcgcacg
     7501 accgtgacca gcgcgtatgc ggtgctggcc gaacgcggct acctcgagac ccggcgcggg
     7561 tcggggtcga tcgccgtcgt gccgggccgc ctggccgcgg cgagccgggt gctctccccc
     7621 gccacggatc tcgcgccgga tgcgatcgat ctgacgtgtg cggcaacgcg ggcccccgcc
     7681 ggaatcctgg aggcgtatgc cgccgccctg gccgatctgc ccagctacct cggcgacaac
     7741 ggatatgccc ccgtcggcct gctggacctg cgagccgtca tcgccgagcg cttcacccgg
     7801 cgcgggctgg cgaccacgcc ggagcagatc atcgtcacct cgggcgcggt tgcgggactg
     7861 gccgtcatct cgcgggcgct gctcacccgc ggccagcggg tgctgctgga gagcccgagc
     7921 tatcccaacg cgatggcggc gctggcgatg gagggcgccc gcacggtggc gatcccggtc
     7981 gacgcggccg ggtgggatct cgacgaggcg gcacggatcg tgccggccag cggcgcgagc
     8041 gcggcatacc tgatcccgga cttccacaac cccactgggg cgctcctcga cgacagcgga
     8101 cgggagcggc tggccgggct gctcgccgcg gcggatgtgc ggccgatcat cgacgagacc
     8161 atcgccgata tcgacctcga cggtggtcca cctgtgcgcc cgttcggctg cttcagcccc
     8221 ggcgccatcg tgctggggtc ggcggccaag gtcttctggg gtgggttgcg gatcgggtgg
     8281 atccgggtgc cgacggcgct ggcttcgcgt gtggtctccg cccgggccac cttggatctc
     8341 ggggcgccga tcctcgagca actcgtgttg atgcatctgc tcgtgaccca tgacgggctg
     8401 cccgcggcgc gccgcaccga actgctcgcg gcccgcgacg ccacggtcga ggcggtaggg
     8461 acgcacctgc cgcacgcgcg cttcgtcgtg ccgcgcggtg gcctgacctt gtgggtggac
     8521 atgggtgatc tgcccgtcgg gggcctggtc cttgccgccg cggaagccgg cctgctggtg
     8581 gcggcgggtc cgcagttcgc gcccgtgggc ggcttcgagc ggttcatccg gatcccctat
     8641 gtcgccgctc cggccgtcat gaccgatgcc gtcgcgcggc tggcccgcgc cgctgaggag
     8701 ttgcgcgccg gcggcgtgac cgcgctgacg catccgccac gcgccggggg cttcgtggcg
     8761 tgagggaggt cggcgcgacg gcctgagagg cttggctcat gagtgacgtc gtcttcgagc
     8821 gtgtgcacca cgtccaggtg gccattcccg tgggtgggga ggattccgct cgtgcgttct
     8881 gggtcgagct tctcggcttt gccgaggtgc cgaaaccgcc ggtcatggcc gcccgcgggg
     8941 ggtgctggtt ccgcgcaccg ggtggcgggc tcgagatgca catgggcgtc gagcagccct
     9001 tcgcccccgc aaggaaggcg cacccgggaa tcctcatcgc cggtctcgac gcgctcgcga
     9061 ccaggctcga ggacgccggg tatgccgtgc gctggtccga cgagatcccg ggaaccaagc
     9121 gcttccacac cgacgacccc ttcggcaacc ggctggagtt catcgacgcc ggggcctagt
     9181 gcgagcctgc tgcgccgacg tggcaacctc agccggcgcc ggtgtcgtcg gggcggagca
     9241 tggcgggctg gagcgctgtc gcggggccgc gctgatagag cagggccggg cggcccgagc
     9301 ggccgtcgcc gctgtcgcgg tcctcggtcg cgacgacgaa accaggcgtc gagagcacct
     9361 tgcgccggaa gttgccgagg tcgggcgccg cgccccagac cgcggcatac accctccgca
     9421 ggtcggcgag cgtgaacggc tcctcgacga actgcgctgc gagagtggtg tattcgatct
     9481 tcgaccggac ccgctccagg gcgtcgcgga ggatctcggc gtggtcgaac gcgagctcga
     9541 cgctcgtctc gtccgcgccg tccaggccga ggtcctccac cggccaccac cgtgcggccg
     9601 cggcgtccga cccggcggtc gggacgtgcc gggcgagccc gggagcgaag gcgacgtggg
     9661 ccactgagac cacgcgcatg cgcgggtcgc ggtcggggcg ggagtaggtg gcgagttgct
     9721 ccaagtggcc ggagaagcgc tcgagcccgg tctcctcggc gagttcgcgc caggcggcgg
     9781 tggccgcgtc ctcgtcgatc tcgacgaagc cacccggcaa cgcccagtgg ccggcgaagg
     9841 gatcctcccc gcggcggaca aggaggacgg cgagggtgcc ttcgtggatg gtgaagaccg
     9901 cgaggtcgac cgtgacggca aagggcgggt gatcgctcat cgggccacct ccactccggt
     9961 cgccacgagt tcggtcaacg ccgcctcggt ggactccggc gcgaccccgg ccgtcagcgg
    10021 cagcaggacc gtggtgcgca agccggcccg agcggcgtcg agcgccgtcg cgcggacgca
    10081 atgatccgtg gcgatgccga ccacgtcgac ctcgtccatc ccttctgccg caagcagatc
    10141 cgccaatgtg cggtggtcgt cggtgatgcc ttcgaacatc gagtaggccg gcgcgcccat
    10201 ccccttgcgc acgtgatggg tgagtcgctc ggcgagccgg ggctcggcat agtcagcccc
    10261 cggcgtgccg gcgacgcagt gggcgggcca ggtcgcggcg aagtcggggt cacccgctgt
    10321 cgcgaagtgg ccgccgttgt cggtctcggg cgagtgccag tcgcgtgagc cgacgatcag
    10381 ccggtagtgg ccggcatacc gatcgatgtg gtcgacgatc gccgcggcga cggcccggcc
    10441 gccggtgacg gcgagcgagc ccccttcgac gaagtcgttc tgcacgtcga cgacgagtag
    10501 tgcggtcgag ctcatcgtca ctcctcctcc attgcggtgg tgtagggctc gccgtccgcg
    10561 acctcgtggg cgcttggcgg tagcgcggcg agggccgcgg ccgcatgggc ccggacctcg
    10621 gcgagactgg gctcgtggac catctcgccg ccgcggatga ccggcacctg caccggccgg
    10681 cccccgtcgg ggatgacgcc gtcgagccgg aagtgctcgc tgccgtcggg cagccggtat
    10741 gccgtcttgc ggccgccggc cgagcccttg ccgctggcct tcttggccac gggccgcagg
    10801 ggggcctcgg cgccggggcg atcgctgacg gcgacgagtt tgtagaccat cccggccgtc
    10861 ggatgtcccg agccggtgac gacccgggtg ccgacgccga agccgtcgat gggggccgcg
    10921 ccgagggcgg tgatgacata ttcgtcgagg tcactggtca cgacgatgcg cgtcttggtg
    10981 gcacccagct cgtccagaag cgttctggcc ctgcgtgatt cgacgtccag gtcaccggag
    11041 tcgatccgga tgccaccgag ttcggggccc gcgacctcca cggcggtgcg gatgccctgc
    11101 tcgatgtcga aggtgtcgac gagcagcgtt gtgccggcgc cgagggccgc cacctggctg
    11161 gcgaaggcgt cgcgctcgct gtcgtgggcg aggatgaagg cgtgggccac ggtgccgacg
    11221 gccggtatgc cgtggagatg gccggcggcg agattgctgg tggtgtcgaa gccggcgaga
    11281 taggcggcgc gcgcggcggc gatggcagcc tgctcgtggg tacggcgact gcccatctcg
    11341 atgatcggcc ggccgcgcgc ggcggtcacc atgcgggcgg cggcgctggc gacggcgatg
    11401 tcgtggttga gcaccgacag caccagggtc tccagaagga ggcactcccc cagcggccct
    11461 tcagccgtca ggaccgggct gcccggcaca taggtctcgc cttcggggta tgcggtgatg
    11521 tccccgctga aacggaagtt cgcgagccgg tcggcggccc tggtggtgat ggccccggtg
    11581 gcttggagga aggcgagttc gtcgtcggtg aagccgaagc cggcgatcat cgggatcagc
    11641 cggccgaggc cggcaagcag gccgtagcgc cggcccggcg ggaggcgtcg ggcaaaggcc
    11701 tcgaagacgg agtggttgtc gacgctgccg tcctcgacga acgaggaata catcgtcagc
    11761 tcgtagcggt cggtcagcat gcccgggctc gcgcccgcgg gttgccgggc cgccgtgctc
    11821 ctcggcgcct gcgtcttcgc catgtcgccc cttcgctcac acagccactg cgctcggcca
    11881 cgttcgcgtg ggtcgagccc tcgttggttt aagtcatagt gaccataaca cgaggggagc
    11941 gaggtcagtc gcgggcgtgc tcggcagttt ggtcgatctc gtcgaagatg atcttgctgg
    12001 ccatccccga cggatagccc ttgcgggcga gcagcccggc gaggcgtcgc acctggacgg
    12061 cccggtcgag gccgtggagg cgtttgaggc gcgagcggac caattcgcgg gcgcgctcct
    12121 cttccagcgc cggggagacg tcgtcgacgg ccgcgcccgc cgtgtccggg tcgacccctt
    12181 tggcccgcag ttctcgccgc agcgccgacg cggccagtcc gctgcggcgc tgcttggagc
    12241 ggacgaacat ctgcgcatag gcggcatcgt cgaccaggcc gacctcggtg agtcggtcga
    12301 ggacgtcctt ggccaccagc ggatcgcagt cgcgtttggc gagcgcctcc tccagctcgg
    12361 cacgggtctt cggcgaattc gtcaactgcc gcaacacgat cgccttcgcc acggcgtgcg
    12421 ggtcgcgggc tgcctctccc cgcgaggcgg ggtgcggctc ctcgtcatcg cgtgcgggct
    12481 gagttgggtc gtcgccggac ggagtggcgt ccggcgacga cccgggcggg ctgtggtgtg
    12541 gcatcccgcc ggcctcgatc cggccgatcg ccgcgcggag cgcggacaga tcggccggtg
    12601 tgtggtcggt catcagaact cgaccgggac ctcggcacct tccgccgggg catccacccg
    12661 cggcccgacg ccgagcttct ccttgatccg cttctccaac tcatcggcca gatccgggtt
    12721 gtccttcagg aaggtccggg cattctcctt gccctggccg agctggtcgc cgtcgtaggt
    12781 gtaccaggcg ccggacttgc gcacgaagcc ctggtcgaca cccatgtcga tcaggccacc
    12841 ctcgcgcgag atacccatgc cgtagaggat gtcgaactcg gcctgcttga agggcgggct
    12901 gaccttgttc ttgacgacct tgacgcgggt gcggttgccg accggctcgt tgccgtcctt
    12961 gagagtctcg atccgccgca cgtccaggcg caccgacgca tagaacttca gcgccttgcc
    13021 gccggtggtc gtctccgggc tgttgtggac catgacgccg tcgacgaagt agttgtgggt
    13081 gccgccgacc tcgatgtcaa acttgcgcat cgaacgcgtc ttgggcttga cgtggacatc
    13141 aagcacatcg gcggggacga gcacctctcg cggcgagacg aactccggcc ggacctcgaa
    13201 ctgaccccgc agaccggtca ggagcttgta gtccatcgac ggatgcacaa acggagcgac
    13261 catctcgagg aagcgcttcg acccttccgt ggtcatctgc agcaccgcct tctgagcggc
    13321 cccgcgcata gacacccgcg agtcgactcc gtgggtgtcg cggagatacg acgaaagccg
    13381 ctcccgagac cctggagtca tggcttcgac gcagaattcg acacgtccac tgcccccgag
    13441 ggttcgctgc tgcagtccct tgctgcgcat ggcgaaagag ccgtcgtcca tgaaccagac
    13501 agcaagcgca agcggggtca acgccttgag atattcgtcg ctcaggtgct tcttgccatc
    13561 gccccagtag acgacccgcc gcaactccgc gagctcggcg agcggcgtga agtcgacgtg
    13621 ctcggcgcca ctgggccgag tagagcggga attcgggata ttgcccagca actcgacctt
    13681 ccagtcgaga taggggattt gcttgggtcc gtgtccgagg cggaagcgaa cgccattgcg
    13741 cccccggcgg ttcggcgaaa ggttcccggc gcccatgagg ctgccgagga cgacctgcca
    13801 ctgctgatcg gacagacggg cggtctcgct caccatgacc cggtcgccgg caatgatctc
    13861 gccggcctcg cgccagccgc ccggggtgcg gatcaggtga ttctcggtgg cggcgaattg
    13921 cgaacgacca ttgccgctcg aacgcgccac cgtgaactgc aagaagcgct cggcgttgcc
    13981 gttgtcgaac cagttcgtga tcggtcgagc gacgatctga tccgtggccg ggtcataggt
    14041 gcgcacctcc acgggggact tggaattgac cagcttgccg atcttctcgg tcgtgccatc
    14101 ggcgagggtg acccgcgtcg agtacgacat gcatccgaac atcactccga tcttttcgcg
    14161 gagctggttg atgaagatcg ctgtggtgcc ggtggaattc agcgcaccgg tgatcttgcg
    14221 cagcgcctgg ctcatcaggc gggcctgcag gccgacgtgg ctgtcaccca tctcgccctc
    14281 gatctcggca cgcggcacca gggccgccac cgagtcgatg acgatgatgt cgagcgcacc
    14341 cgagcggatc agcatgtcgg cgatctcgag cgcctgctca ccggtgtcgg gctggctgac
    14401 cagcagggcg tcggtgtcga cgccgagctt ctgggcgtat tcggggtcga gggcgtgctc
    14461 ggcgtcgatg aaggccgcga tacccccatt gcgctgggcg ctggcaaccg cgtgcagggc
    14521 gaccgtcgtc ttgccgctgc tctccgggcc atagatctcc acgacgcgcc cgcgcggcag
    14581 gccgccgatt ccgagcgcca cgtcgagggc gatcgacccg gtggggatca cctcgatggg
    14641 cgctcgcact tcgtcgccca ggcgcatgac cgcgcccttg ccgtagctct tctcgatctg
    14701 gcccatgacc gtgtcgaggg agcgctgctt gttgtcctgg gtcttgtcga tgtccggttt
    14761 gccggccatg ccacacctct tctttcatgc tcgtggtcgg gcgcgcccgc cttcgacccc
    14821 tcgccggatg gcgacgagat cagacgctag gcgagagcac cgacaacccg gcaccccccg
    14881 gccctgagcc tgtggatgac gcgaagtcga actctcgctt gtggatgaca ctagccgaac
    14941 atctgttcga cgcgcgtgga gacacgccgt acctccacaa ggaggacacc cccgccgcag
    15001 accgcaactt cacccgtcgg taacctcacg ccgcagggat gctgtgcgac tacagggggt
    15061 ggaagtgcgc aacggccagc cgttcgaggc ggcggtccag gagcacggac cacgcgtgct
    15121 gcgcgtgtgc cgggccgtcc tgggccccgg ccacgacgcc gacgacgcct ggtccgagac
    15181 ctttctcgcc gccctgcgcg cgtggccgac actcgatcgc ggcacgaaca tcgaggcctg
    15241 gctggtgcgt gtggcacaac gcaagtgcat cgacgtcatc cgcgcgaagt cacgacgcgc
    15301 gatcccgagt gacgttctcc ccgaaccaga ttcgccacct cctccggcgg acaagagcat
    15361 ctggtatgcc gtggcccggc tccccgagaa gcagcgcctt gcgctggcct accactacta
    15421 cggcggactg ccgcacacgg agacggctga gctgatcggc tccactccgg ccgccgtgcg
    15481 acgcgcggcg agcgacggaa tcgccagtct gcgccgcatc ctcgccgaga acggagaaat
    15541 cgtatgagcg aacagaatcc cggcctggcc gatccgttgc catccctcga cccggcctca
    15601 ccgatcgacg tcgccgacct gacggccctc cacgcgcgcc tgatcgcggg agcgaagacc
    15661 gagggcctga tcgatatcgc ctaccggacc gtcgactccc ccgtcgggcc gctgctcgtc
    15721 gcggccacct ccaccggcct ggtccgcgtc gctttcgaga tcgaggactt cgaccgtgtc
    15781 ctcgcggccc tcgccgccct ggtcagcccc cgcatactgc atacccccca ccgcctcgac
    15841 atcgtcgccc gcgccctcga cgactacttc gccggccgcc ggaccggctt cgacctcccg
    15901 ctcgatcacg ccctcagcag cggcttccgc gagcgcgtgc agcgccatct ggtgcagatc
    15961 ccctacggcc agaccacgac atacggtcag ctcgccgccg atctcggctc gccgcgggcg
    16021 gtgcgcgcgg tgggcaccgc gtgttcgacg aacccgctgc ccatcatcca gccctgccac
    16081 cgtgtcctgc gcggcgacgg agccctgggc ggctacctcg ggggcgccca cgtcaaacgc
    16141 agcttgatcg cgctcgaggc gggcaaccgc tgacgccccg ccagcagcgg ccggccgacg
    16201 acgtgcgcca tacccagccc gctcaacacc cgccccgcca tccgggcccg tgaccagccg
    16261 cggtcggcga gcgccccgca gccggcggcc gcgacgggca acgcgagcgc cgcgcgcgac
    16321 ggcagatcac gcgcatgcgt gcggcggttc gtcgccgcga tggccgatac gagagcgccc
    16381 gcgaacacga ccctccgcgc ttgagccgtc gtcatggacg ccaacggtag tcgttacagt
    16441 ggggcagccc gccctcccgc cgatcgtgag ttccctcccg aatgaccagt gtcaccaccg
    16501 ccgcgcccgg ctcgacgacc gcgcgggcaa gcgggttccg cccggacgtg gaggggctgc
    16561 gggcgatcgc cgtcggcacg gtcctgtggt tccacgcggg cttgggcggc ctgcggggcg
    16621 gattcgccgg cgtggacatc ttcttcgtca tctccggctt cctcatcacc gggcagctgg
    16681 tccgggaggt cgagcgcagt tcgcggatct cgttgccgcg tttctacgcc cgccgggcca
    16741 agcggctctt ccccgccgcg gccacggtcc tgctggccac ggccgtcctg accgtcctgt
    16801 tcctgccgaa gatctcgtgg cgggtgttcg gctgggatat cgccgcgtcg gcggcatatg
    16861 tcgtcaactg gcgcctggcc gaccgcgccg tcgactatct cgcggaggac acctcggtct
    16921 cgccggtcca gcacttctgg tcgctggcgg tcgaggagca gtactacatc gtctggccgc
    16981 tgctgctgct tgccctggcc gtgctcatcc ggcgggcgcg gctgcccgtc cgtccgacga
    17041 tggccatcgg gctcgccgcg atcgtgctcc cctcgctggc ctggtcgatc cacctcaccg
    17101 aggccgatcc cgcccgtgcc tacttcgtca cgacaacgcg cctgtgggag ttggggatcg
    17161 gcgcgctcgt cgccgtgggg gcaacgcggt ggcgaaggat ctccgccccg ctcggtgccg
    17221 cgctggcctg gtcgggcctc gcggtgatcg ccgtgggcct ggtcgcccag agcaccgcca
    17281 ccccgtggcc gggctggcac gccctcgtcc cggtcgcggg cacggccgcg gtgatcctcg
    17341 gcggccacag cgcgggccgg ctcggccccg tccgggtcct cgggacgcgg ccgatggtct
    17401 ggatcggcgg cctgtcctat tcgctgtacc tgtggcactt ccccctcatc gtcgccgcga
    17461 gcgcccatct cggtgagctc acgcggtggc aaggatctgc cgtggcggtt gcgagcgtca
    17521 tgccggcctg gctgacgcat cggttcatcg agaacccgct gcgcttccac ccccgcgtcg
    17581 ccgagcacac ccgcaatgcc ctggtgctcg ggctgacctg ttcccttctc ggggtcgggg
    17641 ccgggtatgc cgtgacccgg gtgggtgcgt cgcccaccct cgacgcggcg caggtcgcgc
    17701 agctgcgggc ggcgcaccag ttcggtggcc aggtgctcgg gtcgggccag cagccgtcga
    17761 gcgccgggat cgacaggcac ccgcaggcga tgacgcccaa gccggccgac gcgccggccg
    17821 acgcgccggt cctctatgcc gacgggtgcc agaccgtgcg caccaccacc gaggtggtga
    17881 cctgtgtcta cggggtcaag ggcgcacgca aggtcatggc gttggtcggc gattccaaag
    17941 cagcgcaatg gttcccggcg ctggagcggt atgcgctcga gcggggctgg gagatccgca
    18001 cttatctgaa gtcggcctgt ccctggagtc cggccctcat cttcgacggc gacaacccga
    18061 cccggcgcta cccgaactgt cagcagtggg gcaaggacgt gctcgcccgg ctcctcggcc
    18121 ccgagaagcc gacggtggtc gtgacgtccg gcatccggtc cgtcgccttc ggcgccgacg
    18181 ggaccgagcg cgagtcggtc atggtcgacg gttatgtcga ctactggacc caactcggcc
    18241 gggccggtgt gccggtgctc gcgctcatgg acacgcccca gccgggccgg atcgtctacg
    18301 agtgcgtcct ggagcacaag gacgactaca tgaccgcgtg ccaaggcgcg tggaacgacg
    18361 ggagcggcgg gcccgcgctg cgggcggcca ccgaccgagt gcccaccagc cgcctggtgg
    18421 atctcaaccc ctgggtgtgc ccggaccgga cctgctggcc ggtgatcggc tccgtgctcg
    18481 tctggcggca aggtagccat ctgacggcga cctacacgga atcgctcgcg tccgtcgtgg
    18541 ctcgcgagat cagtgcggcc ctgacccagg tgggcgtgca gccatgaccg cgtcggcacc
    18601 gacccggggg ttccggcccg aagtcgaggg tttgcgtgcc atcgcggtgc tgacggtcat
    18661 gtggttccac cgcggctgcc cggccttccc ggggggttcg ccgg
//
LOCUS       DAOPJG010000043        18649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3127_length_18649_cov_7.302087, whole genome shotgun sequence.
ACCESSION   DAOPJG010000043 DAOPJG010000000
VERSION     DAOPJG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 18649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18649
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3127_length_18649_cov_7.302087"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..631)
                     /locus_tag="PKU75_05925"
     CDS             complement(140..631)
                     /locus_tag="PKU75_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF80450.1"
                     /translation="MRFVQRRVHPSHAADVVADAFLMAWRRYDEAPPGLDRQRTWAFG
                     IARNCLLNTRRGIDRSEALAVHLAHAVPTPAPPGADPESVVARVNLAAAWRELDAADQ
                     ETLALSALDGLTAPEAGRVLGISPVAYRLRLMRARTTLRRHLDRRSTSTPTDPAEASH
                     ERA"
     gene            793..1299
                     /locus_tag="PKU75_05930"
     CDS             793..1299
                     /locus_tag="PKU75_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPF80451.1"
                     /translation="MSPDLTTHPAVTAVRAALAERGLDPEFRVLPDAVKTAQAAADAL
                     GITPAEIANSLIFKAIDADGSVSPLLVMASGGHRVDTGFLAGQLGIATLERADPDFVR
                     SATGMAIGGVAPVGHPTPVRTVVDRVLERFDQVWAAGGHAHAVFRTSYADLLAMTGGA
                     AADVVAPT"
     gene            complement(1342..1799)
                     /locus_tag="PKU75_05935"
                     /pseudo
     CDS             complement(1342..1799)
                     /locus_tag="PKU75_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     assembly_gap    2277..2376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2377..3100)
                     /locus_tag="PKU75_05940"
     CDS             complement(<2377..3100)
                     /locus_tag="PKU75_05940"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF80452.1"
                     /translation="MIQGTAPVAPPLAGNRNVHLLWFGEGVSVLGSMTTTMVFPLLAV
                     LVFDVGLGPLGLLPAATWLPWLLFSLPAGVWIDRGDPRRIMIAADLASAAVTASVPIA
                     WALGRLTLVHVVLAALGVGAGAVFFRTADSSFVPRVVAKDDLESANSRLFGTESAMQI
                     AGPGLGGALVALVGVAYAVVLDTLSFLVSALCLVPMGPTSWPGPSPLCANRWPRPSVP
                     GCGSSRAIRMCASPRSKAASRIS"
     gene            complement(3650..4516)
                     /locus_tag="PKU75_05945"
     CDS             complement(3650..4516)
                     /locus_tag="PKU75_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF80453.1"
                     /translation="MPQPPDPQDSRAILHPGVMAERVRFERFAAPEEFAGILDWCWSV
                     EWEMPPGESHAQETLSLPAVNISVGTTPPPGPHPPPRPYAVSPRVVGVATRRTTRVLS
                     GSGWNVAAKASVGGFGALYAGRVADLCDLEVPMRQVLAVDDERLGERMASAPDGAERS
                     ASLVAALEPVLAQADPERLRLAREVARIAEVAADQPEMVRVGDLAAYAGMSVRTLQRL
                     FLEYAGVSPSWMLRRLRLIEAAERVARGQDVRWADVAAELGYSDQAHLSRDFTAAVGQ
                     APSAYAAAQRPT"
     gene            4610..5206
                     /locus_tag="PKU75_05950"
     CDS             4610..5206
                     /locus_tag="PKU75_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HPF80454.1"
                     /translation="MNTEATATAEQTRSVLNSLAAVLDGIRDDQTDDPTPCTDYTVAD
                     LRSHVVGWATAFGEGFGDPEGQAPDASSIAVEGSGAEQVRAAGDAIVAGIEGGGAERE
                     LKLADGMAMPGGMALAMTLWEYQVHGWDLAKATGQDWVPADQPLESSITFADQMLTPD
                     FQGEGKPFGPRVDVADDAPALDRLVAMSGRDPEWSPGA"
     assembly_gap    5214..5313
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5314..6189)
                     /gene="metF"
                     /locus_tag="PKU75_05955"
     CDS             complement(5314..6189)
                     /gene="metF"
                     /locus_tag="PKU75_05955"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HPF80455.1"
                     /translation="MSIPTDLRAERPSVSFEFFPPKDDAGEAVLMDAIRRLERARPDF
                     VSITYGAGGSSQDRTVHVTERIARETTLTPLAHLTCVGASVAQLRNVVGQYAAVGIRN
                     ILALRGDPPRGGDGTWVAHPDGLSHADELVRLIRSLGDFTVGVAAFPDVHPESPDFEH
                     DVEVLIRKADAGAEFAVTQMVFDASTYLRLRDAVAARRDLPITPGLMPVTNVKQIARM
                     SELMGTPLPEAVVSRLAAVEGDPEAVRQVGVEIATELAEAMLAEGAPGLHFITMNRST
                     ATLQVYENLGLTAAR"
     gene            6274..7353
                     /locus_tag="PKU75_05960"
     CDS             6274..7353
                     /locus_tag="PKU75_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPF80456.1"
                     /translation="MSAIDLAVLRPRIDAAIEEQLRRAGEDLAELAPESADLLGPITA
                     LLRGGKRLRPLFLYLGYRAAGRPDSDAAVRLAAAMEFVQAAALIHDDVIDASDTRRGM
                     PTTHRALEATHRARGWAGDAASFGVAGAILAGNLCLGWADQSYDECGLPDAELAGGRR
                     TFDLMRAQLMAGQYLDVVSAVRPWAGLAAAERVATAERVITYKSAKYSIQHPLLIGAR
                     TGGVSRDSDGALTAYGLELGIAFQLRDDLLGVFGDPDVTGKPAGDDLREGKRTVLVAH
                     TLAGLDPAGQAEIEGVLGDPGLTPDAVDRARELITASGAVELVEDEITRRADAAAAAL
                     HTAPIAQEARVALGELIATATARSS"
     gene            complement(7360..7734)
                     /locus_tag="PKU75_05965"
     CDS             complement(7360..7734)
                     /locus_tag="PKU75_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HPF80457.1"
                     /translation="MWQAGAVELDDLVGEWLTLPDLAARLGTSPNGVRRLLQERELVG
                     LPRGTPKVLSVPAAFLDGAAPLPALKGTVTVLGDNGFSDAEIIEWLFTADPTLPGGGT
                     HAVDAMRAGFKTEVRRRAMEEM"
     gene            7863..8717
                     /locus_tag="PKU75_05970"
     CDS             7863..8717
                     /locus_tag="PKU75_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPF80458.1"
                     /translation="MVPLLSIPATPPPLFDLPSVAHHTSSGQKHTVRSGETVFGLALR
                     YGTSSSAIAQANDLANSRMIHPGDVLVIPAGDTAAAKRPARSRSAHTVSAGETLTGLA
                     AHYDTTVAAIAKANGISPGRFIMPGQKLSIPGATTRVDQSFESSPKSAPVRGMASRTA
                     TRDLIASTALRYGVDPKLALAISWQESGWQQGVSSHVGAVGVMQVMPGSGTWAGSLIG
                     RSLDLHDLEDNVSAGVVIIKQLTKMATDRDEVIAAYYQGLGSVRERGWYADTKQYVAN
                     VNYFRGKF"
     gene            8842..10830
                     /gene="pknB"
                     /locus_tag="PKU75_05975"
     CDS             8842..10830
                     /gene="pknB"
                     /locus_tag="PKU75_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HPF80459.1"
                     /translation="MNVGPDALIGEVVDGRYTVLERIAAGGMATVYRAVDTRLDRDVA
                     LKVMRPHLAHDESFVARFRREARAAARLSHPNIVSVFDQGEDRGRVFLAMEYVPGRTL
                     RDVIEDDGPLSPDAALDILEPVLLALAEAHHQGGIIHRDVKPENVIIRDDGVVKVADF
                     GLARAVTTETATSASSEVLGTLSYISPEQVEGGKGTPRSDVYAAGLLLFEMLTGRKAF
                     EGDSIPHVIYQHIHHGVPTPSRVHPGIPPNVDSLVLQAAAKDPDDRPQDAAEFLAEVR
                     HVRRLLGDGAREAASTSGPSGAQTSAPLATAVLPVHHDQPATAAALAANETQPYAVTR
                     PPARAVRPAPRPVVAKPRKASAGARAIAAPPAAGKRRRRTGILAGLLALLAGAAAAGW
                     YFLAGPGAPTTVPQLSNLTRAQAHAALETAHLDAKDVQAFSETVAKGVVISATPGEGA
                     SLRRGEDVTLTISKGKERYEVPVVAGITDKTALARLAARNLEGEVTRAYSDTVEKGRV
                     VSSTPVAGTELKRGDKVTIVVSDGPQPIDVPTYRGMTLEEAQAAATAVGLTVAEGEQV
                     YSTDVEQGRIVTQTPAEGQVVRGATITVQVSKGPEMVEVPNVFNLGEAKAVATLEAAG
                     FAVKVEKFLGAPLDVCTGQTPGGGEQAPKGSTVTITIV"
     gene            10827..11759
                     /locus_tag="PKU75_05980"
     CDS             10827..11759
                     /locus_tag="PKU75_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPF80460.1"
                     /translation="MSAPAGPHSDRTRIVATLLLLAMTATWGSTFFLIKDLVAHVPSA
                     DFLVVRFAIAALAMNLVFHRQVMSLPRNAVRTSLLLGALYGTAQILQTVGLEHTSASV
                     SGFVTGAYVVLTPILGALLLRERLPGVMWVAVALATVGLGVLSLRGFAVGLGESLTLA
                     AALLYALHIVGLGRWSTPAEATGMACIQAAVISLVALVAALPGGITLPATGGQWTSLL
                     YMALVAGAFALWAQTWAQAHVHPTRAAIIMTTEPVFAAFFAVLFGGESLTGRMLLGGG
                     LVLAAMYLSELRVRAVHDVIDDERPSEALHHDAP"
     gene            11835..13190
                     /locus_tag="PKU75_05985"
     CDS             11835..13190
                     /locus_tag="PKU75_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPF80461.1"
                     /translation="MSDEAVKYLLDDSEMPTHWYNVVADLPTPPPPPLHPGTHQPVGP
                     DDLSGLFPPELIGQEVTAERFVEIPRAVREVYRLWRPSPLFRARRLEAKLATPARIYY
                     KYEGVSPAGSHKTNTSVPQAYYNSINGITRLTTETGAGQWGTALAFACSLFGMHCDVW
                     QVGASFDSKPARRTLMETFGATVFRSPTRDTESGRAFPEGHPGSLGIAISEAVEVAAQ
                     QDDVKYALGSVLNHVLLHQTVIGEEALLQLAKAGESGADLVIGCAGGGSNLAGLTFPF
                     VREKLAGKQNPRLIAVEPAACPTLTRGEYRYDFGDTAGLTPLMKMYTLGHDFVPSAIH
                     AGGLRYHGMAPLVSHLVHEGHIEAVALHQNECFAAAIEFARTEGIVPAPESSHAIALA
                     RQEALACAESGEDKVIVVGLSGHGMLELGAYESYLAGGLADEALSEEALTVALAGVPQ
                     L"
     gene            complement(13333..14370)
                     /locus_tag="PKU75_05990"
     CDS             complement(13333..14370)
                     /locus_tag="PKU75_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HPF80462.1"
                     /translation="MAPCAVDLLSDTLTRPSAGMRAAMAEAEVGDDVFGEDPTVARLE
                     ARVAEFLGHEAGLFSPTGSLANQLGLRLHVKPGQELIADSMAHILRAELGAAAAFSGI
                     TSRTWVSDRGRPTPEQVLSLMAAEASPYHVATALIELENTHNFGGGSILPLATIEAIR
                     AGSEPHGVAMHLDGARLWNAHVATGIELAAYGGLFDTVSVCLSKGLGAPVGSVLVGSA
                     DAMAEARIWRKRYGAGMRQAGILAAAGLYALDHNIARLAEDHARARRFAEAVGSVAPV
                     VDPAQVETNIVILDLTDHALSAPQAVSGFAQHGVRAYALSPTHVRLVWHLDVDDASTD
                     LAIEAARATLG"
     gene            complement(14370..15695)
                     /locus_tag="PKU75_05995"
     CDS             complement(14370..15695)
                     /locus_tag="PKU75_05995"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HPF80463.1"
                     /translation="MSMLLDGPARPAAQQPVWPDDGALKGAIETLASMPPIVFAGECD
                     LMRQRMAAASRGEAFVLQGGDCAETFVNATADNIRDRVKTLLQMAAVLTYGASVPVVK
                     VGRMAGQYAKPRSSDTETRAGVTHPAYRGDMVNDYDFSADARRPDPQRLVRAYHASTA
                     TLNLVRAFTTGGYADLRHVHEWNKGFVSTPANAAYERLARDIDKAMKFMAACGADFDA
                     MRQVEFFAAHEALLLDYERPLTRIDSRTGKLYDTSGHFIWVGERTRQLDGAHLDFISR
                     VHNPVGVKLSAKSDPAYIEQLMDLVDPEREPGRLTFITRMGAAQVRDALPGVIEAATR
                     AGSAATWICDPMHGNTFESASGFKTRDFDDIVEEVRGFFDVHRALGTVPGGMHVELTG
                     NDVTECLGGAEKILDADLAQRYESVCDPRLNHRQGLELAFLVAEMLARD"
     gene            complement(15806..16450)
                     /locus_tag="PKU75_06000"
     CDS             complement(15806..16450)
                     /locus_tag="PKU75_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF80464.1"
                     /translation="MTLRVLVADDHPLWRDALVADLEKAGYDVVGSAADGPSAVNRTK
                     ATRPDVLLLDLNLPGLHGADVCAQVAGLATRVLILSASGEERDVLAAMKAGASGYLVK
                     SATPEEIRAAVDATGRGEATFTPGLAGLVLGEYRKLASEPAPDERPIPQLTERETEIL
                     RLVAMGLSSKEIGEQLVISHRTVQNHVQNTLGKLQLHNRVELTRFAIEHGLDEP"
     gene            complement(16476..17633)
                     /locus_tag="PKU75_06005"
     CDS             complement(16476..17633)
                     /locus_tag="PKU75_06005"
                     /inference="COORDINATES: protein motif:HMM:NF039922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5931 domain-containing protein"
                     /protein_id="HPF80465.1"
                     /translation="MALGRIRNTGDPGVVGAFTRGLALFRPLALAYAALLAYLRRDEM
                     TRPGVAVAVFAVLGLWSLVAAWRHRVAWQTAAIDLVLAVAGIAATSLAYPRATVAAGA
                     LTLPSIWSSAGVVAAAVRGGARGGLIAAAVIAAADLISVGKPNFATVHNIVLLFLVGG
                     LIGLAVTLAREGQRVSEISIRAEEAYAERERLARIVHDGVLQALTFINRRGTQIGGPA
                     TELADLAAEQERALRSLVSGRPRLDDTAAVSDLALVLRTLDDDRVTVSTPAEPVTLPA
                     HRAGELRAAVVAALDNVRDHAGPDARAWVLLEEIGGDVVVTVRDNGAGMPPGRAEEAA
                     ASGRLGMASSIRGRLRDLGGEATWTSAPGTGCTVVLTAPKDLEPLAPRRSA"
     gene            complement(17637..18485)
                     /locus_tag="PKU75_06010"
     CDS             complement(17637..18485)
                     /locus_tag="PKU75_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPF80466.1"
                     /translation="MLYWLLKHILVGPILRVLFRPDVQGLENIPAEGPAILASNHLSF
                     SDSFFLPLVAPRRITFLAKSDYFTGRGLKGRLTAAFFRGVGQVPIDRSGGNASNAALT
                     SGLRILHAGDLLGIYPEGTRSPDGHLYRGRTGVARMALEGKVPVIPVAMIDTDKAQPT
                     GQVVPKIHRIGVKIGAPLDFSRYEGMEEDRFVLRSITDEIMYALMELSGQEYVDVYAS
                     AQKKRLLAVARDRAKDLESRLPHRHAAADEPALGVVEGRGVADAPDDNTGDLEPPSGT
                     ALPRAS"
BASE COUNT         2782 a         6488 c         6560 g         2619 t
ORIGIN      
        1 cggcgcggcg gcgctcggcg cgggcgcggc atacggatcc gtggcgacga tgcgcgacag
       61 caggtccgcg gcgcgatcgc gttgtgccgc agtgatttcc ggcgtagccg ggtccaggct
      121 gcgcaaggtg gtgtgcaggt caggcgcgtt catgggatgc ctccgcgggg tcggtgggag
      181 tcgaggtcga gcggcggtcg agatgacggc gcaaggtggt ccgtgcgcgc atgaggcgca
      241 ggcggtaggc caccggggag atgccgagga cacgaccggc ttcgggcgcg gtcaggccgt
      301 cgagagcgga aagggcgagc gtctcctggt cggcagcgtc gagttcgcgc caggccgccg
      361 cgagattcac gcgcgcgacc acggactcgg gatccgctcc gggtggcgcc ggcgtcggca
      421 cggcgtgcgc gaggtggacg gcgagcgcct ccgagcggtc gatgccccgc cgggtgttga
      481 ggaggcagtt gcgggcgatg ccgaaggccc aggtccgttg tcgatccagc cctgggggcg
      541 cttcgtcgta gcgacgccag gccatgagga aggcgtcggc gacgacatcg gcggcgtgcg
      601 acggatggac ccggcgctgg acgaagcgca gcaggtcagg gatgagtcgc ggtgaagaat
      661 gcccggaagg ccgcctccca ctccgcggtc cccgggcgtt gtgatcgtgt catgtcccct
      721 acgtgtcctg cgacgctccc actgtttcgg cgcgcggtgc gacgaccggc ggcgccgcat
      781 acagtctgat ccgtgagccc cgacctgacc acgcaccccg ccgtgaccgc cgtccgcgcc
      841 gcgctcgccg agcgcgggct cgatcccgag ttccgggtgc tgcccgatgc cgtcaagacc
      901 gcgcaggccg ccgccgatgc cctcggcatc acccccgcgg agatcgcgaa ttcgttgatc
      961 ttcaaggcga tcgacgccga cgggtcggtc tccccgctgc tggtgatggc ttctggcggg
     1021 caccgcgtcg acaccggctt cctcgccggg caactcggga tcgccacgct ggagcgcgcg
     1081 gacccggact tcgtgcgctc cgccaccggt atggcgatcg ggggcgtcgc cccggtcggt
     1141 cacccgactc ccgtgcgcac cgtcgtcgac cgcgttctgg agcgcttcga tcaggtgtgg
     1201 gcggcgggcg ggcacgccca tgcggttttc cgcacgtcgt atgcggacct gctggccatg
     1261 accggcggcg cggccgccga cgtggtcgct cccacctgac cgggcgtcgg cgcggcgggc
     1321 gcccggcacc ctcgaaaaga gttatgcgcc cccggacccc tcggccaccg cggccaatcg
     1381 ctcgatcgag gcgagcaggc ggtcgctcgt ggtccaacgg gccctggcca tgcggccctc
     1441 gccggtcagg tcccgccagt cataggtgtg gcgcaccagc gtgtgggcgg cgtcgacggg
     1501 ttcgagctcc cacatccacc gatggccgaa aggcgcggtg ccgacacccc cgggcgccag
     1561 gcgatgaggc ggccttcctc gaaggcgtcg acgtgattga cgcgcacatt gcctgtgcgc
     1621 gtgttcatca cgaagatctc ccccgccgcg cggactcgct gacccgaatc tgcttgcaac
     1681 agattgtcat tcccgtccca ctccggttgc cgcgccggtt ccgcgatcag gtcgaagatc
     1741 acctcggcgg gagcggcgac aacctgctcg gcgctgacca ccttcgtctc agccaccagt
     1801 ccagtatgcc gcgcaccagg ccgagcatcg gcatacgggc tggtcagcgt gccggcagcc
     1861 gcatgggctc ttccgggagc ggccggccgg gccggtaggg gccgatgagc accgagagcg
     1921 atgcgaggcc gtgcaggatc gccatggcgg cgagcgtccg gcgcaccccg aggtgggtgc
     1981 cgagccagcc tcgccgccag gccgccgagc ggcatggtgc ccatagtgat cagggcggac
     2041 gcggagacga cccgcgccat caccgcagtc gggacatagc gcatccggaa ggcagcgcgc
     2101 aggacgttgg cgccgacgac gccgaccccg acgagcgccg cgccgagggg gacgagcccg
     2161 acgagcagac ccggccgggc ggcggcgatg agcagcgcgg tggggccgcc gaggatctgc
     2221 agccaggcca tggcgggccc ggtgccgagg cgtgcggcgg cgcggcggcg cggcggnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcga aattcgagac gccgccttgg
     2401 accgcggcga agcgcacata cggatcgcgc gtgaggatcc gcagcccggc acggacggac
     2461 gtggccatcg gttcgcgcag agcgggctcg ggccgggcca gctggtcggg cccatcggga
     2521 ccaggcacag cgcggagacg aggaaggaga gggtgtcgag gaccacggca tacgcgaccc
     2581 cgacgagagc gaccagggcg ccgccgagcc cgggcccggc gatctgcatc gccgactcgg
     2641 tgccgaagag acgcgaattc gcgctctcca gatcgtcttt cgcgacgaca cgcgggacga
     2701 acgagctgtc tgcggtgcgg aagaagaccg cgccggcccc gacgccgagg gccgcgagga
     2761 cgacatggac gagggtgaga cggccgagcg cccaggcgat ggggacgctg gcggtgacgg
     2821 cggcggaggc gaggtcggcg gcgatcatga tgcgtcgggg gtcgccgcga tcgatccaga
     2881 caccggcggg gaggctgaag aggagccacg gcagccaggt ggcggcgggg agcaggccga
     2941 gcgggccgag gccgacgtcg aagacgagga cggcgagcag cgggaagacc atggtggtgg
     3001 tcatcgagcc gagaaccgag acgccctcgc cgaaccagag caggtggacg ttccggttgc
     3061 cggcgagggg cggggcaacc ggtgcggtgc cctggatcat ggctgtgcgg ggaagccgcg
     3121 cgcgaagacg atgcaggacc gcgctcggcg ccctcctcgg ggatctcccg ctcgctccat
     3181 cgcttcagga cggccatgat ctcctcggcg acggcataca tctcctgcgg ggagagcgtg
     3241 agccagttct gggtggcgaa ggccgcgtcg tcccagacgg ggtcggagtc ggcattggcc
     3301 atccactgct gcacgcgctc gacctggcgg cgcaactgca gcacttcggc ttcgcgggcg
     3361 agggctagcg cggcggcgtc gtctccgaat tccgatgtgg tccaacgtgt tccggcgctg
     3421 accaagcgcc accagcgctc gcgcttgtcc ttggcgagtt cgggtgcctc ctcgaccagg
     3481 ccggcgccgg cgagcacctt caggtgatgg ctcgcgttgc ccacggcctg cccggtgcgc
     3541 ttggcgagca tcgacgcggt cgacgggccg tcgaccttca gcgcgtccag catccgggcc
     3601 cggaagggat gagcgagcgc ggttgtatgc ggctggaaca gccgcgacct caggtcggcc
     3661 gctgagcagc ggcgtaggcg ctcggggcct ggccgaccgc ggccgtgaag tcccggctca
     3721 ggtgtgcctg atcgctgtag cccagttcgg ccgcgacatc cgcccaccgc acgtcctgac
     3781 cgcgagccac gcgctcggcg gcttcgatga gccgcaaccg gcgcagcatc cacgacgggg
     3841 agacgccggc atactccaga aagagccgct gcagagtccg cacgctcatc ccggcgtatg
     3901 ccgcgaggtc gcccacgcgc accatctcag gttggtccgc cgccacctcg gcgatgcgcg
     3961 ccacctcccg cgccagccgc agcctctccg gatcggcttg cgccagaacg ggttcaagtg
     4021 ccgccaccag cgacgccgaa cgctcggcac cgtcgggcgc cgaggccatc cgctccccca
     4081 gacgctcgtc gtcgacggcc agcacctgac gcatcggcac ttccaggtca cacagatcgg
     4141 cgacccggcc ggcatacagc gccccgaaac cgccgacact cgcctttgcg gcgacattcc
     4201 agcccgagcc gctgagcacg cgcgtggtgc ggcgggtggc gacgcccacg acgcgggggc
     4261 tgacggcata cggcctgggc ggcggatgcg ggcccggcgg cggcgtggtg ccgacgctga
     4321 tgttcacggc cggcagggac agcgtttcct gcgcgtgcga ctcccccggc ggcatctccc
     4381 actccaccga ccagcaccag tcgaggatgc ccgcgaactc ctccggcgcg gcgaaccgct
     4441 cgaaccggac gcgctcggcc atgacccccg ggtgcagaat ggcgcgggag tcctgcggat
     4501 cgggcggctg cggcacaggt gtgagcctac gagcgccgcg ccggcctacg acgaaatctg
     4561 tcgcgattct tcaagcccgg cccgagcgcg cggccatacc gtcgaagaca tgaacaccga
     4621 agccacggcc accgccgaac agacccgcag cgtcctgaac tccctcgcgg ccgtcctcga
     4681 cggcatccgc gacgaccaaa ccgacgaccc gaccccctgc accgactaca ccgtcgccga
     4741 cctgcgcagt cacgtcgtcg gctgggcgac cgccttcggc gaggggttcg gcgacccgga
     4801 aggccaggcg cccgatgcct cgtcgatcgc ggtcgagggc agcggcgccg aacaggtgcg
     4861 cgcggccggc gacgcgatcg tcgcgggcat cgagggcggc ggcgccgagc gtgagctcaa
     4921 gctcgccgac ggcatggcca tgccgggcgg gatggcgctg gcgatgacgc tgtgggagta
     4981 ccaggtgcac ggctgggacc tcgccaaggc caccggccag gactgggtcc ccgccgacca
     5041 gccactcgaa agctcgatca ccttcgccga ccagatgctc acgcccgact tccagggtga
     5101 gggcaagccg ttcgggccgc gcgtcgacgt cgccgacgat gcgccggctc tcgatcggct
     5161 cgtcgccatg tccggccgcg accccgagtg gagccccggc gcctgagacc tgannnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcagcgg gcggcggtca ggccgaggtt
     5341 ctcgtagacc tggagggtcg cggtggagcg gttcatcgtg atgaagtgca gcccgggagc
     5401 gccctcggcg agcatggcct cggcgagctc ggtggcgatc tccacaccga cctggcgcac
     5461 cgcctcggga tccccctcga cggccgccag ccgcgagacc acggcctccg gcagcggggt
     5521 gcccatcagc tcgctcatcc gggcgatctg cttgacgttc gtgaccggca tcagtccggg
     5581 ggtgatcggc aggtcccggc gggcggcgac ggcatcccgc agccgcagat aggtcgaggc
     5641 gtcgaaaacc atctgtgtca cagcgaattc ggcgcccgca tccgccttgc ggatcagcac
     5701 ctcgacgtcg tgctcgaaat cgggtgactc ggggtggacg tcggggaagg ccgcgacacc
     5761 gacggtgaaa tcaccgagcg agcggatcaa ccgcaccaac tcgtcggcgt gcgagaggcc
     5821 gtccggatgc gcgacccagg tgccgtcgcc gccgcggggc gggtcccccc gcagggcgag
     5881 gatgttgcgg atgccgaccg cggcatactg cccgacgaca ttgcgcaact gcgccaccga
     5941 ggcgcccacg caggtgaggt gggcgagcgg ggtcagggtc gtctcgcgag cgatccgctc
     6001 ggtgacgtgg acggtgcggt cctggctgga gccgcccgcg ccgtaggtga tggagacgaa
     6061 gtcgggccgc gcccgttcga ggcgacggat agcgtccatg agcacggcct cgccggcatc
     6121 gtccttcggc gggaagaact cgaagctcac actcgggcgc tcggcccgca gatcggtggg
     6181 gatggacacg gtgtccaagg atagatggcc tgctcgcccg gtccgtgcag gacgttaggg
     6241 tgtgggacgc cccaggggaa ggattccgcc tcgatgtccg ccatcgatct cgccgtgctc
     6301 cgcccgcgga tcgacgccgc gatcgaggag caactgcgtc gtgccggcga ggacctggcc
     6361 gagctggcgc cggagtcggc cgacctgctc ggcccgatca ccgcgctgct gcgtggcggc
     6421 aaacggctgc gtccgctgtt cctctacctc ggctatcggg cggccgggcg gcccgacagc
     6481 gatgccgcgg tccggctcgc cgcggcgatg gagttcgtcc aggccgccgc gctcatccac
     6541 gacgacgtga tcgacgcctc cgacacgcgc cgcggtatgc cgaccaccca ccgcgcgctc
     6601 gaagcgaccc accgcgcgcg gggctgggcg ggcgacgccg cgagtttcgg ggtggccggg
     6661 gcgatcctcg cggggaacct gtgcctcggc tgggccgatc agtcgtatga cgagtgcggg
     6721 ctccccgacg ccgagttggc cgggggccgg cgcacgttcg acctcatgcg ggcccagttg
     6781 atggccggcc agtacctcga cgtcgtctcg gcggtccgcc cctgggccgg cctggctgcc
     6841 gcggagcggg tggcgacggc cgagcgcgtg atcacgtaca agtcggcgaa gtactcgatc
     6901 caacacccgc tgctcatcgg tgctcgcacg ggcggggtga gccgggactc cgacggcgcg
     6961 ctcacggcat acggcctgga gctcgggatc gccttccagc tgcgcgacga cctgctcggc
     7021 gtcttcggcg acccggacgt cacgggcaaa cccgcaggtg acgacctgcg cgagggcaaa
     7081 cgaaccgtcc tggtcgccca caccctcgcc ggcctggatc ctgccggtca agcggagatc
     7141 gagggtgtcc tcggcgatcc ggggctcacg ccggacgcgg tggaccgggc gcgcgagttg
     7201 atcaccgcct cgggtgcggt ggagctcgtc gaggacgaga tcacccggcg ggccgacgcg
     7261 gccgcggccg cactgcatac cgcccccatt gcccaggagg cccgcgtggc actcggcgaa
     7321 ctcatcgcaa cggcgaccgc gcgcagcagt tgagtccgct cacatctctt ccatagcgcg
     7381 gcggcgcacc tcggtcttga acccggcccg catcgcgtcg acggcgtgtg tgcccccgcc
     7441 cggcaacgtc gggtccgcgg tgaagagcca ttcgatgatc tctgcatcgg agaacccgtt
     7501 gtcgccgagg acggtcacgg tgcccttcag cgccggcagc ggtgcggcgc cgtcgaggaa
     7561 agcggccggc acgctcagga ccttgggggt accgcggggc aatccgacca gctcgcgctc
     7621 ctggagcagg cggcgcaccc cattgggcga ggttccgagg cgggcggcga ggtcgggaag
     7681 ggtcagccac tcgccgacca gatcatctag ctccacggcg ccagcctgcc agatcacgaa
     7741 acgcttctcc ggccaacgac gcgacaccac accagacaca caacatacgt tcagtacctt
     7801 ctgtactctt tcgcaacaac agtcacaact tcatcgatct ccaccggtcc ggaagggacg
     7861 ccatggttcc cctgctcagc atccccgcga cgcccccacc gctgttcgac ctcccctcgg
     7921 tcgcccacca cacctccagc ggacagaaac acacggttcg ctcgggcgag acggtgttcg
     7981 ggctggccct gcgctatggc acgagcagca gcgcgatcgc ccaggccaac gacctggcga
     8041 actcgcggat gatccacccc ggtgacgtgc tggtcatccc ggcgggcgat accgccgcgg
     8101 ccaagcgtcc cgcccggagc aggtccgctc acaccgtctc ggccggggag acgctcaccg
     8161 gcctcgccgc ccattacgac accacggtcg ccgcgatcgc caaggccaat ggcatctcgc
     8221 cgggccggtt catcatgccc gggcagaagc tgtcgatccc cggcgccacc acccgggtcg
     8281 accagagctt cgaatccagc cccaagtcgg cgccggtccg cggcatggcc tcgcgcaccg
     8341 ccacccgcga cctgatcgcc tcgaccgccc tgcgctacgg cgtggacccc aagctcgcgc
     8401 tggccatcag ctggcaggag tccggctggc agcagggggt cagttcccac gtcggtgccg
     8461 tcggggtcat gcaggtgatg ccggggtccg gcacctgggc cggctcgctc atcgggcgct
     8521 cgctcgacct gcacgacctc gaggacaatg tctccgccgg cgttgtcatc atcaagcagc
     8581 tgacgaagat ggccacggac cgcgacgagg tcatcgccgc ctactaccag gggctggggt
     8641 cggtgcgcga gcgcgggtgg tatgccgaca ccaagcagta cgtcgccaac gtcaactact
     8701 tccggggcaa gttctgagtc accccacgac gttctccacg cgtccctcgc tccgatgctt
     8761 cgcgcggacc ccgacacagg actcaccccc gagcacacgc acctgagaga tcccgacagg
     8821 aaggacccct agaatttgcc ggtgaacgtg ggcccggatg cgctgatcgg cgaggtcgtc
     8881 gacggccgct acaccgtgct cgaacggatc gccgccggcg gcatggccac ggtctaccgg
     8941 gccgtggaca cacggctgga ccgcgatgtc gccctgaagg tgatgcggcc gcacctggcc
     9001 cacgacgaga gcttcgtcgc ccgcttccgc cgtgaggcca gggccgccgc gcgcctttcg
     9061 caccccaata tcgtctccgt cttcgaccag ggcgaggacc gggggcgggt cttcctcgcc
     9121 atggagtatg tgccgggccg gacgttgcgc gatgtcatcg aggacgacgg cccgctcagc
     9181 ccggacgccg cgctcgacat cctcgagccg gtgctgctcg ccctcgccga ggcccaccac
     9241 cagggcggca tcatccaccg cgacgtcaag cccgagaacg tcatcatccg cgacgacggc
     9301 gtggtcaagg tcgccgactt cggcctggcc cgggccgtga cgaccgagac ggcgacctcc
     9361 gcctccagcg aggtcctcgg caccctctcc tatatctctc cggaacaggt cgagggcggc
     9421 aagggcactc cacgcagcga tgtctatgcg gccgggctgc tgctgttcga gatgctcacc
     9481 gggcgcaagg cgttcgaggg cgactccatc ccgcacgtca tctaccagca catccaccac
     9541 ggcgtgccga ccccgtcgcg ggtgcacccc ggcatcccgc cgaacgtcga ctcgctcgtg
     9601 ctgcaggccg ccgcgaagga tcccgatgac cgcccccagg atgcggcgga gttcctcgcc
     9661 gaggtgcgcc acgtgcgccg gctcctcggt gacggcgcac gcgaggcggc gtccaccagc
     9721 gggccgagcg gggcgcaaac ctccgccccc ctcgcgaccg ccgtgctgcc ggtgcatcac
     9781 gaccaacccg cgaccgctgc ggctctcgcg gcgaacgaga cgcagccgta tgccgtcacc
     9841 cggcctccgg ctcgtgccgt gcgtcccgcg ccgcgaccgg tggtcgccaa gccacgcaag
     9901 gcgtccgcgg gagcgcgggc gatcgccgcg ccaccggcag ccggcaagcg gcgccgacgc
     9961 accggcatac tggcgggcct gctcgccctg ctcgcgggcg cggcggccgc cggctggtac
    10021 ttcctcgccg gtcccggtgc ccccaccacc gtgccgcagc tgtcgaatct aactcgggca
    10081 caagcacatg cggctctgga gacggcgcac ctcgacgcca aggacgttca ggcgttcagc
    10141 gagacggtgg cgaagggcgt ggtcatctcc gctacccccg gcgagggcgc ctcgttgcgg
    10201 cgcggcgagg acgtcaccct gacgatctcc aagggcaagg aacggtatga ggtgcccgtc
    10261 gtcgcaggca tcaccgacaa gaccgcgctg gcgcgcttgg cggcgcgcaa tctcgaaggg
    10321 gaggtcacgc gggcctactc cgacacggtc gagaagggcc gggtcgtctc ctccacaccg
    10381 gtcgcgggca ccgagctcaa gcgcggagac aaggtcacga tcgtcgtcag cgacgggccg
    10441 cagccgatcg acgtgcccac ctaccggggg atgacgctcg aggaggcgca ggccgcggcg
    10501 accgccgtgg gcttgaccgt cgccgagggt gagcaggtct actccaccga cgtcgagcag
    10561 ggccggatcg tgacccagac accggccgag ggccaggtcg tgcggggggc cacgatcacc
    10621 gtccaggtct ccaagggccc tgagatggtc gaggtgccga acgtgttcaa cctgggcgaa
    10681 gccaaggccg tggccaccct cgaagcggcc ggcttcgcgg tcaaggtcga gaagttcctc
    10741 ggggcgcctc tcgacgtgtg caccggccag accccgggcg gcggggagca ggccccgaag
    10801 ggttcgaccg tcaccatcac gatcgtatga gcgctcccgc cggaccacac tccgaccgga
    10861 cgcggatcgt cgcgacgctc ctgctgctcg cgatgaccgc cacgtggggc tccaccttct
    10921 tcctcatcaa ggatctcgtc gcgcacgtgc cgtcggcgga cttccttgtg gtgcgcttcg
    10981 ccatcgccgc gctcgcgatg aacctggtgt tccaccgcca ggtgatgtcg ttgccgcgca
    11041 acgcggttcg cacctccttg ttgttgggtg cgctctacgg cacggcgcag atcctgcaga
    11101 ccgtcgggtt ggagcacacc tcggcgtcgg tgtcgggttt cgtcaccggg gcatatgtcg
    11161 tgctgacccc gatcctcggc gcactgctgt tgcgcgagcg gttgccgggg gtgatgtggg
    11221 tggccgtcgc tctggcgaca gtcggcctgg gggtgctgtc gttgcgcggg ttcgcggtgg
    11281 ggctcggcga gtcgctgacg ctggccgccg ccttgctcta tgcgctgcac atcgtgggcc
    11341 tgggccgttg gtcgacgccc gccgaggcga ccggtatggc gtgcatccag gccgccgtga
    11401 tctcgctcgt cgcgctcgtg gcggccctgc cgggcggcat cacgctgccg gcaaccggag
    11461 gccagtggac ctcgctgctc tatatggcgc tggtcgccgg cgccttcgct ctgtgggcgc
    11521 agacgtgggc gcaggcgcac gtgcacccga cccgggcggc gatcatcatg accaccgagc
    11581 cggtgttcgc cgccttcttc gccgtgctct tcggtggcga gtcgctgacc ggccggatgc
    11641 tgctgggcgg cgggttggtg ttggcggcga tgtatctgtc cgagctgcgc gtccgcgccg
    11701 tccacgacgt catcgacgat gagcgtccct ccgaagcact ccaccacgac gcaccctgag
    11761 atgcccgttt cgcgagggag tgggcgccgc tagggtgagc agccgacccc accgatccga
    11821 tgccgggagc gctcgtgtcc gatgaagccg tgaagtacct cctcgacgac tccgagatgc
    11881 cgacccactg gtacaacgtc gtcgccgacc tgccgacgcc tcctcccccg ccgctgcacc
    11941 ccggcaccca tcagccggtc ggcccggatg acctgtccgg actcttccca ccggagctca
    12001 tcggccagga ggtgaccgcc gagcgtttcg tcgagatccc ccgagcggtg cgcgaggtct
    12061 accgactctg gcggccctcg ccgctgttcc gggctcgccg attggaggcc aaactcgcca
    12121 cgcctgcccg catctactac aaatacgagg gcgtctcgcc ggccgggtcg cacaaaacga
    12181 atacgtctgt gccgcaggcc tattacaact ccatcaatgg catcacccgc ctgacgacgg
    12241 agaccggcgc cggccagtgg ggcacggccc tggccttcgc gtgcagtctc ttcggaatgc
    12301 actgcgacgt ctggcaggtg ggcgcgtcct tcgactccaa gcccgcccgg cgcacgttga
    12361 tggagacctt cggcgccacc gtcttccgct cccccacccg cgacaccgaa tccgggcgcg
    12421 ccttccccga gggccacccg ggcagcctcg gcatcgccat ctccgaggcc gtcgaggtcg
    12481 ccgctcaaca ggacgacgtg aagtacgccc tcggctcggt gctcaaccac gtcctgctgc
    12541 accagacggt gatcggtgag gaggcgctgc tgcagctggc caaggcgggc gagtccggcg
    12601 ccgacctcgt catcggctgc gccggcggcg gctccaacct ggccggcctg acgttcccct
    12661 tcgtccggga gaagctcgcg ggcaagcaga accctcgtct gatcgccgtc gagcccgcgg
    12721 cctgcccgac cctgacccga ggcgaatacc gctacgactt cggagacacg gccggtctga
    12781 cgccgctgat gaagatgtac accctgggtc acgacttcgt cccctcggcg atccacgccg
    12841 gcggcctgcg ctatcacggc atggcaccgc tcgtatcgca cctggtccac gagggccaca
    12901 tcgaggcagt cgccttgcac cagaacgaat gcttcgctgc cgcaatcgaa ttcgcgcgca
    12961 ccgaggggat cgtgccggcg ccagagtcgt cccacgcgat cgccttggcc cgccaggagg
    13021 ccttggcgtg cgcagagtcc ggagaggaca aggtcatcgt cgtcggcctg tccgggcacg
    13081 gcatgctcga gttgggcgcc tacgagagct acctggccgg cggacttgcc gacgaggcgc
    13141 tctccgagga agccctcacg gtcgcgctcg ccggggtgcc tcagctctga ggcggctcct
    13201 cctccgaccc gggccggctt gtcgtcgtgg ggcctggaaa tagccaggcc ccataacgag
    13261 aacctggccc gtctcgacga gaacctggcc ccgcaacgac aaccaggccc ggcaacgagg
    13321 agctggccgg cgtcagccca gggttgcccg ggcggcctcg atcgcgaggt cggtgctggc
    13381 gtcgtcgacg tcgaggtgcc agacgagccg gacgtgggtg gggctgagcg cgtaggcgcg
    13441 gacgccatgc tgggcgaagc cggagacggc ctgcggcgcg ctgagcgcgt ggtcggtcag
    13501 gtcgaggatg acgatattgg tctcgacctg cgccggatcg accacgggcg caacggaacc
    13561 cacagcctcg gcgaagcggc gcgctcgcgc atgatcctcg gcgaggcgcg cgatgttgtg
    13621 gtcgagggca tagagtccgg ctgcggcgag gatgccggcc tggcgcatcc ccgcgccata
    13681 gcgcttgcgc cagatccgtg cctcggccat ggcgtcggcg gacccgacga gcaccgaacc
    13741 gaccggtgcg ccaaggcctt tggagaggca caccgagacg gtgtcgaaga gcccgccgta
    13801 tgccgcgagc tcgattcccg tcgccacgtg ggcgttccag aggcgggcac cgtcgaggtg
    13861 catcgccacc ccgtgcggct cggagccggc gcggatcgcc tcgatggtgg cgagcggcag
    13921 gatgctgccg ccgccgaagt tgtgggtgtt ctcgagttcg atgagcgcag tggccacgtg
    13981 atacgggctc gcctcggcgg ccatcagcga gagcacctgc tccggcgtcg ggcggccccg
    14041 gtcggagacc caggtgcgcg atgtgatgcc cgaaaaggcc gctgccgcac cgagttccgc
    14101 gcgcaaaatg tgcgccatcg aatcggcgat cagctcctgc ccgggcttca cgtgcagccg
    14161 cagtccgagc tgattcgcga gggagccggt cggcgagaac aatccggcct cgtgcccgag
    14221 aaactccgcg acccgcgcct cgagtcgcgc gaccgtgggg tcctcaccga agacgtcgtc
    14281 gccgacctct gcctcggcca tggcggcgcg catccccgcc gaggggcggg tcagtgtgtc
    14341 ggaaaggagg tcgacggcac acggcgccat cagtcgcgag ccagcatctc ggcgacgagg
    14401 aaggccagct cgagaccctg ccggtggttc aggcgggggt cgcacacact ctcataccgc
    14461 tgcgcgaggt cggcgtcgag gatcttctcg gctccgccga ggcattcggt gacgtcattg
    14521 ccggtcagtt cgacgtgcat accgccgggc accgtcccga gcgcacggtg cacgtcgaag
    14581 aagccgcgca cctcctcgac gatgtcgtcg aagtcgcgcg tcttgaaccc actcgcggac
    14641 tcaaaagtgt tgccgtgcat gggatcacag atccacgtcg cagcagaccc agccctggtg
    14701 gcggcctcga tcacgcccgg gagcgcgtcg cgcacctgcg cggcgcccat ccgggtgatg
    14761 aaggtcagcc ggccgggttc gcgctcggga tcgacgagat ccatcagctg ctcgatatag
    14821 gcgggatcgg atttcgccga cagcttcacc cccacggggt tgtgcacgcg tgagatgaag
    14881 tcgaggtgcg ccccatcgag ctgccgggtg cgctccccga cccagatgaa gtgaccggag
    14941 gtgtcataga gcttgccggt gcgcgagtcg atgcgggtca gcgggcgctc gtagtcgagc
    15001 agcagcgcct cgtgcgccgc gaagaattcg acctggcgca tcgcgtcgaa gtccgctccg
    15061 caagcggcca tgaacttcat cgccttgtcg atgtccctgg caagccgttc gtatgccgca
    15121 ttggccggcg tcgagacgaa gcccttgttc cactcgtgga cgtgccgcag atcggcatac
    15181 ccgccggtgg tgaaggcccg aacgaggttg agagtcgccg tggaggcgtg gtaggcacgc
    15241 acgagccgtt gcgggtccgg gcggcgggcg tcggcgctga agtcgtagtc gttgaccatg
    15301 tcgccgcgat aggccgggtg cgtcaccccc gcccgggtct cggtgtcgct gctacgcggc
    15361 ttggcgtact ggccggccat cctcccgacc ttgaccaccg gcaccgacgc gccataggtc
    15421 aggacagccg ccatctgcag gagcgtcttg acgcggtcgc ggatgttgtc agccgtggca
    15481 ttgacgaagg tttcggcgca gtcgccgccc tggaggacga aggcctcacc ccgggaggcg
    15541 gccgccatgc gctgtcgcat cagatcgcat tcgccggcga agacgatggg tggcatcgag
    15601 gcgagagtct cgatcgcgcc cttgagagcg ccatcgtcag gccacaccgg ctgctgcgcg
    15661 gcagggcgcg cgggcccatc gagcaacata ctcatcggag cggcggtcgt cgtcacccgg
    15721 acaggatatg tggctgttca gtatccggac gccccgcccg tataccggaa gccggccggg
    15781 cgaaggagtg acatctcgac tggggtcagg gctcgtcgag gccgtgctcg atggcgaagc
    15841 gggtcagctc gacccggttg tgcaactgca gcttgccgag agtgttctgg acgtggtttt
    15901 gcacggtccg gtgggagatg acgagttgct cgccaatctc cttgctggac aagcccattg
    15961 cgaccagccg caggatctcg gtctcgcgct cggtcagttg cgggatgggg cgttcgtcgg
    16021 gggcgggctc gctcgcgagc ttgcggtact cccccagcac gaggccggcc agacccggag
    16081 tgaatgtggc ctcaccccgg ccggtcgcgt cgacggcggc ccggatctcc tcgggggtgg
    16141 ccgacttgac gaggtagccg gatgcgccgg ccttcatcgc ggccagcaca tcccgctcct
    16201 ccccgctcgc cgacaggatg agcacccggg tcgccagccc ggccacttgg gcgcagacgt
    16261 cggcgccgtg caggcccggc aggttcaggt ccagcaggag cacgtccggc cgggtggcct
    16321 tggtccggtt caccgcggac gggccgtccg cggcggagcc gacgacgtcg tagccggcct
    16381 tctcgaggtc ggccacgaga gcatcacgcc agagcgggtg gtcatcggcg acgaggacac
    16441 gcagggtcat gagcaggctc tttccgggac gacggtcatg cggaacggcg gggtgcgagg
    16501 ggttccaggt ccttgggggc ggtcagcacg acggtgcacc ccgtgccggg cgcggaggtc
    16561 cacgtcgcct cgccgccgag gtcacgcagc cggccccgga tgctcgaggc catcccgagg
    16621 cgtcccgagg cggcggcctc ctcggcgcgg ccgggcggca taccggcccc gttgtcgcgg
    16681 acggtgacca cgacgtcgcc gccgatctct tcgagcagca cccacgcccg cgcgtcgggg
    16741 ccggcgtgat cgcgcacgtt gtcgagcgcg gccacgacgg ccgcccgcaa ctcacccgcc
    16801 cggtgcgccg gcagcgtcac cggctccgcg ggcgtgctca ccgtgacccg gtcgtcgtcg
    16861 agggtgcgca gcacgagggc gaggtcactc acggcggccg tgtcgtcgag gcgggggcgc
    16921 ccgctgacga gcgagcgcag ggcccgttcc tgctccgcgg cgagatcggc gagttccgtt
    16981 gcgggacccc cgatctgagt gccgcggcgg ttgatgaagg tgagcgcctg cagcacgccg
    17041 tcgtgcacga tccgggcgag gcgctcccgc tcggcatacg cctcttcggc gcggatggag
    17101 atctccgaca cgcgttggcc ctcccgcgcg agggtgaccg cgagcccgat caagccgccg
    17161 acgaggaaga gcagcacgat gttatgcacc gtcgcgaaat tcggcttccc gaccgagatc
    17221 aggtcggccg cggcgatgac ggccgccgcg atcagaccgc cgcgggcgcc gccgcggaca
    17281 gcggcggcga cgacgccggc gctcgaccag atgctgggca gcgtcagcgc gcccgccgcc
    17341 acggtggccc ggggataggc caggctggtg gccgcgatgc cggccacggc gagcacgagg
    17401 tcgatggcgg ctgtctgcca ggcgacccgg tgacgccagg cggccacgag cgaccagagg
    17461 ccgagcacgg cgaacaccgc gaccgcgacg ccgggacggg tcatctcgtc gcggcggaga
    17521 taggccagca gcgcggcata cgccagggcc aggggccgga acagcgccag gccgcgggtg
    17581 aaggcaccga caacgccggg atcaccggtg ttgcggatgc ggcccagggc catcggtcag
    17641 ctcgcgcggg gcagggccgt gcccgagggc ggctcgaggt caccggtatt gtcatccggc
    17701 gcgtccgcga cccccctgcc ctcgacgaca cccagggccg gctcgtcggc agctgcgtga
    17761 cgatgcggca gccgcgactc gaggtccttg gcccggtcgc gcgccacagc aaggagtcgc
    17821 ttcttctgcg ccgaggcgta gacgtcgaca tactcctggc cggacaactc catcagcgcg
    17881 tacatgatct cgtcggtgat cgagcgcagg acgaagcggt cctcctccat gccctcgtag
    17941 cgcgaaaagt caagcggcgc accgatcttc acgccgatgc gatggatctt ggggacgacc
    18001 tgcccagtcg gctgcgcctt gtcggtgtcg atcatcgcga cggggatgac cggcaccttg
    18061 ccttcgagcg ccatgcgcgc gacgccggtg cggccgcgat agaggtgccc gtcggggctg
    18121 cgggtgccct cggggtagat gccgagcagg tcgccggcgt gcaggattcg caggccgctg
    18181 gtgagtgcgg cgttgctcgc gttgcctccg gagcggtcga tgggcacctg cccgacgccc
    18241 cggaagaagg cggcggtcag ccggcccttg agtccgcggc cggtgaagta gtcggacttg
    18301 gcgaggaacg tgatgcgccg cggcgcgacc agcgggagga agaaggagtc gctgaacgag
    18361 aggtgattgc tggccaggat ggccggaccc tcggcaggaa tgttctccag accctggacg
    18421 tcgggtcgga agaggacccg caggatcggc cccacgagga tgtgcttgag gagccagtac
    18481 agcacgctgg tcgcctttcc ttcggtgggt cgactctacg gcacccagcc cctgacgcga
    18541 ggactcctcc gctgttcggg gccgcgcgtc ggacatgtca ccagcgagct ggcggccatg
    18601 gcccgcgagt cgtatgccgt gcgaggataa ggcgtgcccg agcccgatc
//
LOCUS       DAOPJG010000044        18597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3138_length_18597_cov_7.658020, whole genome shotgun sequence.
ACCESSION   DAOPJG010000044 DAOPJG010000000
VERSION     DAOPJG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 18597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18597
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3138_length_18597_cov_7.658020"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="PKU75_06015"
     CDS             <1..163
                     /locus_tag="PKU75_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HPF80467.1"
                     /translation="FDSAEVGAYCGSGITAARMALALRQIGVTPAVYVGSWSEWITDP
                     DRPVATGAE"
     gene            175..471
                     /locus_tag="PKU75_06020"
     CDS             175..471
                     /locus_tag="PKU75_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80468.1"
                     /translation="MSPQAAPQQVPQPRNASERALVALARLPRPVPVLGVLVLLAIGA
                     FVPTYGWIATALVALFLAWILSLSWPRLTGVEKIMRIAVIAFVAGLAVIQSRPR"
     gene            complement(449..1282)
                     /locus_tag="PKU75_06025"
     CDS             complement(449..1282)
                     /locus_tag="PKU75_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80469.1"
                     /translation="MRPSLVHGLVEVPRMAGEVGEYAAFRRILRKARHGDGQHVLVLP
                     GLMAGDYTTAIMRSYLSSLGYHPHGWGVGRNIGPTRLIVDSMRASVENLTAEHGPISL
                     IGWSLGGLYARQLGRAYPDQVRQVITLGSPFRLIHREDTLASLVFDRYRHLHVEAEEL
                     DMPEQEHLRDPMPIPTTAIYSRTDGIVPWQACLNEIYGKAENIEVKASHIGMGHHPAV
                     LWAIADRLALPMDQWRPFKVPGSLRRWYPTPLSWPLDDEFLIDVIEPDLGGPVNEAAT
                     G"
     gene            1373..2359
                     /locus_tag="PKU75_06030"
     CDS             1373..2359
                     /locus_tag="PKU75_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HPF80470.1"
                     /translation="MFVSRLRRMTALPALVALTGAFTGGLAGCGDPATGSGGGRTQVV
                     VGFYAAQYLAERVGGDRVGVTNLTAPGVEPHDLELTARQVVRLAKADLVITVPGFQPA
                     VDEGIVAAQARRVLDLAPVVGVDLAGGESHDAQPHAEDGHAAAGHAHETPADLAHDPH
                     FWLDPHLMADASAAVAAQLSAADPGGTGLYEANSAALQADLETLEAEIKSRLHDCRQQ
                     DLVTSHAAYGRLAAITGFVPHPLALNPEAEPSPAQLAEITDLVRNEGIATIYVEPLAN
                     RRVAEVVAAETGARTAVLDPIEGGGAIGSPQDYLGLMRANVATLAAGQECLA"
     gene            2356..3156
                     /locus_tag="PKU75_06035"
     CDS             2356..3156
                     /locus_tag="PKU75_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF80471.1"
                     /translation="MSPVIELTDAAFGYAGQPAVSHVTFRVDPGEVVAVLGPNGSGKS
                     TLIKGLVGLTEELGGTTTILGQPLAQAERGLVGYVPQRHTLGTSVRASVAEIVGTGRV
                     AFMSMWRPWARNPARSRQAVARALDLVGLGDRADADVSALSGGQQRRVLIARALASSP
                     EVLIMDEPTAGVDHGNQLVLAGVLERLKARGTTMLIVTHELDALATVVDRVVVIEDGR
                     LTYDGPADSLPPGGTRGHHHDHDDEDTGESAGAPVIPTARIDLGGPRA"
     gene            3149..4213
                     /locus_tag="PKU75_06040"
     CDS             3149..4213
                     /locus_tag="PKU75_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPF80472.1"
                     /translation="MLEILDYAFMRNALLAALLIGVAAPLVGIFMVQRRLSLIGDGLG
                     HVALAGVAVGILTSSSPVLTALLTAVAAAVAIEFTRARGGPTSDLALALMFYGGIALG
                     VVLIGISPEGTPANLTAYLFGSITATTTGDLYVFLALTLVVLLVTWALRPWLFAAAQD
                     EEYARAAGMPVTAVNLILSILTAVTVVVSMRVVGLLLISALMVIPNAAAQAIARSFRG
                     AMTFAVGLGLISAVGGVITSFYAETPSGGTIVLLALALFVVVSVIAAWQRRRDAAHAR
                     AERHDHEHGPGCGHEAIRHKDHMDYLHDGHRHAAHGGHYDEHAPDEHAPDEHAGDGHA
                     GDEHATGARARRAHARGAGS"
     gene            4210..4611
                     /locus_tag="PKU75_06045"
     CDS             4210..4611
                     /locus_tag="PKU75_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPF80473.1"
                     /translation="MSERPRRVTRQQQAITAALQGRDDFRSAQELHAQLRSGGATIGL
                     ATVYRTLQALADEGLIDMLRTEGGESLYRACSRGHHHHLVCRTCGRTVEVEADDAERW
                     AIRTATAYGFTDVEHTLEIFGRCASCSGSGG"
     gene            4627..6567
                     /locus_tag="PKU75_06050"
     CDS             4627..6567
                     /locus_tag="PKU75_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPF80474.1"
                     /translation="MSRALVLAIVGMLLLTAAGIGSFVWLRGDASDAERSARTYAGGV
                     AQALADGTLPGGLEVADRAGAEQDYDEVLRGMGSLRPTVSVSKVDLANDERTGTVTFA
                     QSWTIHEGKKPWTYTTTMPITRRGSGEHLAWTGTWSHAVIAPELTADERLSAVRDKAV
                     RGDILAEDGTALVTLRPVVRVGIDKTLAKKGSDVSATAAALAEAVGIDPAAYRKAVTA
                     AGSKAFVEAIVYRSESSDLAQLSNIAKKYPEMRLLEDELPLAPTSGFARPILGTVGPA
                     TAEIIEKSAGRIRQGSLVGLSGLEAAQDQRLRGSDGFTVNAVQIGSDETRELYAVSAV
                     DGLPVTVSLDEAVQEAAEAALTDVGDKGGASALVAIRPSDMHVLAAASGAGSQGYSTS
                     TLGQYPPGSTFKAVTALALLRSGLTPKTTIDCPKTATVEGRAFKNYDDYPPKRLGSIS
                     LTSAIANSCNTALISQLDRLDGADLAEAAAALGLTAQPQLGVPGALGTVPADAAGVDL
                     AASLIGQGRVVTSPMGMATVAASIARGSIGAPVIVIDGREPKPSPPSKPLTRKESEEL
                     QALLRAVVTDGSASFLKGVPGDDVLAKTGTAEYGSGDPPPTHAWMIGIQGDVAVAVFV
                     EKGQSGAKTAGPILEEFLRRMP"
     gene            complement(6581..7351)
                     /locus_tag="PKU75_06055"
     CDS             complement(6581..7351)
                     /locus_tag="PKU75_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPF80475.1"
                     /translation="MIPPFPHPSGAKPPRYAAADLPKHVAVVMDGNGRWANQRGLPRT
                     KGHEAGEAALLDVVAGAIEVGVTHLSAYAFSTENWKRSPDEVRFLMGFNRDVIRRRRD
                     QLHEWGVRMRWVGQRPRLWGSVIKELEVAQELTQDNTGLTLYFCVNYGGRAEIASAAK
                     RIAEQVAAGRLKPGGVDERLLARYLPEPDMPDVDLFVRSSGEQRTSNFLLWQSAYAEM
                     VFLDTLWPDFDRRHLWQAVDLYVNRERRYGGAVDAVST"
     gene            complement(7348..8133)
                     /gene="recO"
                     /locus_tag="PKU75_06060"
     CDS             complement(7348..8133)
                     /gene="recO"
                     /locus_tag="PKU75_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPF80476.1"
                     /translation="MPTYRDQAIVLRTHKLAEADRIVTVLARERGKLRVVAKGVRRTK
                     SRFGARLEPGMVVDLQCYEGRNLDTVTQAVSIASHGEQIARDYRAYTALSTVLETADR
                     LTEEKAPALQQFLLLQGALAALAGGAHDASLVLDAYLLRALAIGGYAPSFHDCAQCGD
                     PGPQLAFNVAAGGAVCPSCRRPGSVAPARATLDLLAALLAGDWDVADASAAQHRREGS
                     GITAAYVQWHLERGLRSLPHVEREPHAAPPQNESLSLGAGSGS"
     gene            8263..8892
                     /locus_tag="PKU75_06065"
     CDS             8263..8892
                     /locus_tag="PKU75_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HPF80477.1"
                     /translation="MTLQDVLDFGDGPAAAGAPFGPLAPVRTPLSYGAWVDVAPGWLR
                     AADELFEALRADVPWQAERRQMYDNVVDVPRLTRFYGESEVWPHVLLPEICRAISDHY
                     LPTLGEPLVTAGLCLYRDGADSVAWHGDRIGRSRFQDTIVAILSLGSTRTLALRPRGG
                     GTSIRLTSGHGDLIVMGGSCQRTWEHAIPKTREPVGPRMSVQFRPAGVR"
     gene            complement(8985..10766)
                     /gene="leuA"
                     /locus_tag="PKU75_06070"
     CDS             complement(8985..10766)
                     /gene="leuA"
                     /locus_tag="PKU75_06070"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPF80478.1"
                     /translation="MIHPQHPTPMPFTKYLPFQQQIQVELPDRTWPDKVMTQAPRWCA
                     VDLRDGNQALIDPMDAERKLRMFKLLVKMGYKEIEVGFPSASQTDFDFVRELIEGGHI
                     PDDVTIQVLTQCRDHLIERTYDAIRGSKQAIVHFYNSTSVLQRRVVFGMSKEGIIDIA
                     LQGARLCRKLEETIPETTVYYEYSPESYTGTELEFAVEICNAVIEVIDPTPDHKMIVN
                     LPATVEMATPNVYADSIEWMVRHLDRRDSVVVSLHPHNDRGEGVAAAELGYLAGADRI
                     EGCLFGNGERTGNVCLVTLGMNLYSQGIDPQIDFSDMADIRRTVEHCNQLSVHERHPW
                     GGDLVYTAFSGSHQDAIKKGFEDMDRRSAAAGTDVNGIDWGVPYLPIDPHDIGRSYEA
                     VVRVNSQSGKGGVSYLLKSEHGLDLPRRLQVEFSQVVQRRTDAEGGELTAAEIWEMFA
                     DEYLHSDNPQTRWGRFFPVRSILTSTDVGDDHIESAILDNGAEVHISGSGNGPIDAFI
                     DAMSALDVDVRVLDYHEHALSAGGDARAAAYVECAVGERVLWGVGLHESIVKASLRAI
                     MSALNRAERDQAATKDELAVSRPGGGP"
     gene            complement(10974..11405)
                     /locus_tag="PKU75_06075"
     CDS             complement(10974..11405)
                     /locus_tag="PKU75_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPF80479.1"
                     /translation="MIVLDAYAVIGLLRGDEVASEVRRLLEDESCAINSVNLAEVVDQ
                     LVRVFGRPEDDVLADLALLQLTGMRVIPVDAAVATSAGLTRAANYGSKRCPVSMADCV
                     AAATASATDAALATADPDLANVARAGGTPVVALPDSDGRLP"
     gene            complement(11402..11647)
                     /locus_tag="PKU75_06080"
     CDS             complement(11402..11647)
                     /locus_tag="PKU75_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPF80480.1"
                     /translation="MSPSTAKISRNGQVSLPAEVRRRWHAERVVVVDRGDYVIVRPVP
                     DDILAAVSGAHAGPGPATRSARADERAREADRDRSRR"
     gene            complement(11685..13067)
                     /locus_tag="PKU75_06085"
     CDS             complement(11685..13067)
                     /locus_tag="PKU75_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPF80481.1"
                     /translation="MSDEAFRTVIEPFRIHSVEPLRMTTAEHRRQVAREAGYNLFQIR
                     SENVLIDLLTDSGTGAMSRDQWAAIQHGDESYAGSPSYFVFRDAVRELFPYEHVIPTH
                     QGRAAEKILFGALGGVGKVVPSNTHFDTTRANIEFTGAEALDLVIAEGHDPSSLHPFK
                     GNMDTARLEALLAERGDNVPCVMMTITNNSGGGQPVSLENLRAVSEIAHAHGKPLFLD
                     ACRFGENAWFIREREEGQGERDVPDIIRDMAACADGMTMSAKKDPMGNIGGWLALNDD
                     DLAQQCRSVLILTEGFPTYGGLAGRDLEALAQGLKECVDHDYLRYRIRSTAYLADALD
                     KLGMPCLKPAGGHAVYIDARALLPHIEPLHFPGQALAVALYEAGGVRSCEIGSVMFGR
                     HPDGSESPAAMELVRLAIPRRTYTQSHIDYVIEVCERVKARAGDLPGYRIVDEPPALR
                     HFTATFEQFG"
     gene            complement(13090..13854)
                     /locus_tag="PKU75_06090"
     CDS             complement(13090..13854)
                     /locus_tag="PKU75_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HPF80482.1"
                     /translation="MTSRRALIRRPGPRLAHGLLTHIERDPVDGELAIGQWEAYAHAL
                     REAGWETLEVPPADDCPDAVFVEDPVVVHEDLAVIANPGAAQRRRETIGVEQTMRDLG
                     YRIARIAAPGTLDGGDVLRSGDTAYVGLSSRTNGAGIQQLATHLREFGVRTQAVPVER
                     ALHLKSAVTALPDGTVIGWDPVVDDKEAFPAYEAMPEEGGAHVVILGEDQLLMAASAP
                     RSAELLRARGYAVTTVDISEFEKLEGCVTCLSVRLR"
     gene            complement(13875..14390)
                     /locus_tag="PKU75_06095"
     CDS             complement(13875..14390)
                     /locus_tag="PKU75_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPF80483.1"
                     /translation="MADLKRPAFDADHRTSLLGWFQLQREIVVLKTDGLAEADVHRVV
                     IPTSPLMTVGGLLSHLRWCEHLWFQVAYSGVAESENPMFDDDPDDNEFIVGQGKPLDQ
                     LVAEYEEECRRSDAVIAAAGLDDRGAGTTFPAAGATLGWMIHHMFEETARHAGHLDLI
                     RELLDGEKSYF"
     gene            14452..15357
                     /locus_tag="PKU75_06100"
     CDS             14452..15357
                     /locus_tag="PKU75_06100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80484.1"
                     /translation="MPLPPVPAGLIARPLEASDLDATYAVYVADEFVNTGQRMVEWED
                     IESDWQRPSFDLSTDSVGIFYGDRLVGAAEVFAGRRADGAVAPDWHGRGIGTWLLAWT
                     EHRAKEQGSSLVGQSKFGGSPAEALLRGRGYHEMWTSWVLEVPEDQTITPQPLPDGYA
                     LREFTAADEHAAYHLIEDAFNEWPDRDPATFEDWRPRIIGRAGFSPWQIRFVTDPGGK
                     PVGVAAIILDSQQCGYVDQLAVRKDHRGKGLARALLVDAFEEARRRGATRSELSTDSR
                     TGALGLYEKVGMVVTQTWRHWAREL"
     gene            complement(15479..15955)
                     /locus_tag="PKU75_06105"
     CDS             complement(15479..15955)
                     /locus_tag="PKU75_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010552008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPF80485.1"
                     /translation="MSEPSTPAIAHAQALGLDYTVTEHGPVSSLAEAAAVRGIMPADI
                     VKTLVVRRADDDYLFVLVPGDREISWPKLRALLGVSRLSMPDAGTAKAATGYERGTIT
                     PFGSSTRWPVVVDATIPGRRVSMGSGRHGVALGVAADDLVRVFEAQVADISEPQAP"
     gene            complement(15952..16977)
                     /locus_tag="PKU75_06110"
     CDS             complement(15952..16977)
                     /locus_tag="PKU75_06110"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HPF80486.1"
                     /translation="MKLSDFPRYPLTFGPSPVHHLRRLSDALGGAQVWAKREDVSSGL
                     AFGGNKVRKLEYIVPDILESGADTLVSIGGYQSNHTRQVAAVAAHLGLKCRLVQEKWV
                     PWEDPVNDKVGNILLTRMMDADARLDPHGFDIGIRDSWKAALREVEESGGTPYPIPAG
                     ASEHRLGGLGFANWAFEVAEQEKSLGVHFDTIIVCTVTGSTHAGMIAGFAALEDLTGI
                     RRRVLGIDASATLDQTREQVERIARHTAELIELGRDLRDDEIQVLEGWAGDLYGVPVE
                     STMEAMAMGAQLEAMITDPVYEGKSLAGLIELVRTKDIPADSTVLYAHLGGQPALNAY
                     HSLWPAT"
     gene            complement(16980..17636)
                     /locus_tag="PKU75_06115"
     CDS             complement(16980..17636)
                     /locus_tag="PKU75_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPF80487.1"
                     /translation="MPLSDATVPSRRSLLRESVYEQLRDAIVDGSLAPGEKLNDQELA
                     ARLGVSRTPVREALLRLAQSGLVVADPGKSTVVADLDPTAVRHARDVVASMRSLAVRE
                     SVGRLTPEDVEAMTQASERYAAALADGDIDAALRADDDLHAVPVVLADNRAIDAVLAQ
                     FTPVIRRVERARFDSFAVQDSIARHAEFIHLCAAGDALAASDHEFETWRTLTHDGRRA
                     "
     gene            complement(17748..18014)
                     /locus_tag="PKU75_06120"
     CDS             complement(17748..18014)
                     /locus_tag="PKU75_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HPF80488.1"
                     /translation="MADITDFESAVAAVPGLTRDPGNDTKLRLYALYKQATEGDVSGK
                     RPGFTDFVGRAKYDAWKGLVGTDPAAAEDEYIQVVNELVVGETP"
     gene            complement(18156..18281)
                     /locus_tag="PKU75_06125"
     CDS             complement(18156..18281)
                     /locus_tag="PKU75_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80489.1"
                     /translation="MPGNAGKVPQFPKKGGGKQERDRNKDGSWREKRSDAGKKRT"
     gene            complement(18325..>18597)
                     /locus_tag="PKU75_06130"
     CDS             complement(18325..>18597)
                     /locus_tag="PKU75_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HPF80490.1"
                     /translation="QLAFYDAVADNESAREVMGDEVLATIARELVAVMRRDIKTDWTV
                     RDDVRAKLRSSIKRLLVKYRYPPDRQPEAIRQVIEQMEAMAPRLAA"
BASE COUNT         2797 a         6416 c         6449 g         2935 t
ORIGIN      
        1 cttcgacagc gccgaggtcg gcgcctactg cggcagcggc atcaccgcgg cgcgtatggc
       61 gttggcactg cggcagatcg gcgtcacacc ggcggtgtat gtcggcagct ggagcgagtg
      121 gatcaccgac ccggaccggc cggtcgccac cggcgccgag tagccttgcc cctcgtgtcc
      181 ccgcaagccg caccgcagca ggtcccccag ccccgcaacg ccagcgagcg cgccctcgtc
      241 gctctcgccc gtctcccccg gccggtgccg gtgcttgggg tgctcgtcct gctcgccatc
      301 ggggcgttcg tgccgaccta cggctggatc gccaccgcgc tcgtcgcgct cttcctggcg
      361 tggatcctca gcctgagctg gccccggctg accggggtgg agaagatcat gcggatcgcc
      421 gtgatcgcct tcgtcgccgg cctcgcggtc atccagtcgc ggcctcgttg accggacctc
      481 cgaggtccgg ttcgatgacg tcgatcagga actcgtcgtc gagcggccag ctcagcggcg
      541 tcgggtacca ccggcgcagg ctgcccggca ccttgaacgg gcgccactga tccatcggca
      601 acgccaaccg gtccgcgatg gcccagagca ccgcaggatg gtgtcccatc ccgatgtgac
      661 tggccttgac ctcgatgttc tcggccttgc cgtagatctc gttgaggcag gcctgccacg
      721 ggacgatccc gtcggtgcgg gagtagatgg cggtcgtcgg gatcggcatc gggtcgcgca
      781 gatgctcctg ctcgggcatg tcgagttcct cggcctcgac gtgcaggtgc cggtagcggt
      841 cgaagacgag agaggcgagc gtgtcctcgc ggtggatcag ccggaacggc gagccgagcg
      901 tgatgacctg gcgcacctgg tcgggatagg cccggccgag ctggcgagca tacagaccgc
      961 cgaggctcca cccgatgagg gagatcgggc cgtgctcggc cgtgaggttc tccacgctgg
     1021 cgcgcatcga gtcgacgatg aggcgggtcg gtccgatatt gcgcccgacg ccccagccgt
     1081 gcgggtgata gcccagcgag gacaggtagc tgcgcatgat cgcggtggtg tagtcccccg
     1141 ccatcagccc cggaaggacc agaacgtgct ggccatcgcc gtggcgcgcc ttgcgcagga
     1201 tgcgccggaa cgccgcatac tcgccgacct cccccgccat gcggggcacc tcgacgagcc
     1261 catggaccaa cgacggacgc atgaggacag tattacgcct cgacctcgcg gggaggctac
     1321 tcggagcaag gtgggattga cattacatcc gcaacaactg agaatgattc tcatgttcgt
     1381 ttcgcggctt cgtcgtatga cggcgctccc cgccctcgtc gcgctcaccg gggcgttcac
     1441 cggcgggctg gccggatgcg gcgatccggc aacggggtcg ggcgggggcc gcacgcaggt
     1501 ggtcgtgggc ttctatgccg cgcagtacct ggccgagcgt gtcggcggcg accgggtcgg
     1561 cgtcaccaac ctgaccgcgc caggggtcga gccgcacgat ctcgaactga ccgcgcggca
     1621 agtggtgcgg ctggccaagg cggatctcgt gatcaccgtg ccggggttcc agccggccgt
     1681 cgacgagggc atcgtcgccg cgcaggcgcg gcgggtgctc gatctcgcac cggtcgtcgg
     1741 cgtcgacctg gccggtggcg agagccacga cgcccagccg cacgcggagg acggccacgc
     1801 ggcggcgggg cacgcccacg agacgcccgc ggacctcgcg cacgacccgc acttctggct
     1861 cgatccgcac ctgatggccg acgcgagcgc ggccgtcgcc gcccagctga gcgccgcgga
     1921 ccccggcggc acgggcctgt acgaagcgaa ttccgctgcg ctgcaagcgg atctggagac
     1981 tcttgaagca gagatcaaaa gccggctcca cgactgccgg cagcaggacc tcgtcaccag
     2041 ccacgcggca tacggccgtc tcgcggccat caccgggttc gtcccgcacc cgctcgccct
     2101 caatccggag gccgagccca gcccagcgca actcgccgag atcaccgatc tcgtccgcaa
     2161 cgaagggatt gcgaccatct acgtcgaacc gctagccaac cggcgcgtcg ccgaggtcgt
     2221 cgccgccgag accggcgccc ggacggccgt gctcgacccc atcgagggcg gcggcgccat
     2281 cggctccccg caggactatc tggggttgat gcgtgccaat gtggccactc tcgcggccgg
     2341 tcaggagtgc ctcgcatgag cccggtgatc gaactcaccg atgcggcttt cgggtatgcc
     2401 gggcagcccg ccgtctccca tgtcaccttc cgcgtcgacc ccggcgaagt ggtcgcggtg
     2461 ctcggcccca acgggtccgg caagtcgacc ctcatcaagg gcctggtggg cctgaccgag
     2521 gagttggggg gcacgacgac catcctcggc cagcccctgg cacaggccga gcggggcctg
     2581 gtcggctatg tcccgcaacg gcatacgctc ggcacctcgg tgcgcgcgag cgtcgcggag
     2641 atcgtcggca ccggtcgcgt cgcgttcatg tcgatgtggc ggccgtgggc gcgcaacccg
     2701 gcccggagcc ggcaggccgt cgcccgggcg ctggacctgg tcggcctcgg agaccgcgcc
     2761 gacgccgatg tctcggccct ttccggcggc cagcaacgcc gagtcctcat cgcccgggcg
     2821 ctggcgagtt ctccggaggt cctcatcatg gacgaaccga cggcgggggt cgaccacggc
     2881 aaccagctcg tcctcgccgg ggtactcgag cgactcaagg cgcgcggcac caccatgctc
     2941 atcgtcacgc acgaactgga cgctctcgca acggttgtcg accgtgtcgt cgtgatcgag
     3001 gacgggcggc tcacctacga cggacccgcg gacagcctgc cacccggcgg cacgcgggga
     3061 caccaccacg accacgacga cgaggacacc ggcgagagtg cgggagcacc cgtcataccc
     3121 acggctcgga tcgacctcgg aggtccacgt gcttgagatt ctcgactatg cgttcatgcg
     3181 caacgccctg ctggcggcgc tgctcatcgg cgtcgcggcc ccgctggtgg gcatcttcat
     3241 ggtccagcgg cggctctccc tcatcggtga cgggctcggt cacgtcgcgc tcgcgggcgt
     3301 cgccgtcggc atcctcacct ccagttcccc ggtgctgacc gcgctgctca cggccgtggc
     3361 ggcggccgtg gccatcgagt tcacgcgcgc ccggggcggg ccgaccagcg acctggccct
     3421 ggcgttgatg ttctacggcg ggatcgcgct cggtgtcgtc ctgatcggga tctctcccga
     3481 gggcacgccg gccaacctga ccgcctacct gttcgggtcg atcacggcga cgacgacggg
     3541 cgacctgtat gtcttcctcg ccctgacgct cgtggtgctg ctcgtgacct gggcgctgcg
     3601 gccgtggctg ttcgccgccg cccaggacga ggagtacgcc cgggcggcgg gcatgccggt
     3661 caccgcggtg aacctgatcc tgtccatcct caccgccgtg acggtcgtgg tgtcgatgcg
     3721 cgtcgtcggg ctgctcctga tcagcgccct gatggtcatc cccaatgccg ccgcgcaggc
     3781 gatcgcccgc tccttccgcg gcgcgatgac attcgccgtc gggctcggcc tgatctcggc
     3841 cgtcggtggc gtcatcacct ccttctatgc cgagaccccg tccggcggca cgatcgtgct
     3901 cctggcgctc gcgctcttcg tcgtggtcag cgtgatcgcc gcctggcagc gccggcgcga
     3961 cgccgcccat gcccgcgccg aacggcacga ccacgagcac gggcccggct gcgggcacga
     4021 ggcgattcgc cacaaggacc acatggacta tctgcacgac ggccaccggc acgccgccca
     4081 cggcggacac tatgacgaac acgcgcctga cgaacacgcc cctgacgaac acgccggcga
     4141 cggacacgcc ggcgacgaac atgccactgg cgcacgcgcc cgtcgcgcac acgcccgggg
     4201 ggcggggtca tgagcgagcg accgcgccgg gtgacccgcc agcaacaggc catcaccgcg
     4261 gccctccagg gccgcgacga cttccggtcc gctcaagagt tgcatgctca attgcgctcc
     4321 ggcggagcga caatcggctt ggctacggtc tatcgcacgc tccaggccct cgcggacgag
     4381 ggcctgatcg acatgctgcg caccgagggc ggcgagtcgc tgtatcgcgc gtgcagccgc
     4441 ggacatcatc accacctggt gtgccgaacg tgtggacgaa cggtggaggt cgaagccgac
     4501 gatgccgaac ggtgggcgat ccggaccgcg acggcatacg gtttcaccga tgtcgaacac
     4561 acactggaga tcttcgggcg ctgcgcttcc tgcagcgggt ccggcggata gggggcagcg
     4621 atgcgcgtga gccgtgcact cgttctggcg atcgtcggga tgctgctgct caccgcggcg
     4681 ggcatcggga gtttcgtgtg gctgcggggc gatgcctccg acgccgagcg cagcgcgcgg
     4741 acgtatgccg gtggggtcgc ccaggcgctc gccgacggca ccttgccggg cggtctggag
     4801 gttgccgacc gggccggggc cgagcaggac tacgacgagg tgctgcgcgg catgggttcg
     4861 ctgcgaccca cggtgagcgt gtccaaggtc gatctcgcca acgacgagcg gaccggcacg
     4921 gtgacgttcg cgcagtcctg gacgatccat gagggcaaga agccgtggac ctacacgacg
     4981 acgatgccga tcacgcggcg gggttcgggt gaacacctcg cgtggaccgg cacgtggtcg
     5041 catgcggtca tcgcccctga actcacggcc gacgagcggc tgagtgcggt gcgggacaag
     5101 gctgttcgtg gcgatatcct cgccgaggac ggcactgcgc tggtcacgct gcgcccggtg
     5161 gtgcgggtgg gcatcgacaa gaccctggcc aagaagggct ccgacgtctc ggcgacggcc
     5221 gcggccctgg cggaggcggt ggggatcgac ccggcggcat accgcaaggc ggtgacggcg
     5281 gcggggtcga aggccttcgt ggaggccatt gtctatcgaa gcgagtccag cgacctggcg
     5341 cagctgtcca acatcgcgaa gaagtacccc gagatgcggc tgctcgagga cgagctgccg
     5401 ctcgcgccga cgagtgggtt cgcccggccc atcctcggca ctgtcgggcc ggccaccgcc
     5461 gagatcatcg agaagtcggc cggccggatc cgccagggct cgctcgtcgg actcagcggg
     5521 ctcgaggccg cccaggacca gcggctgcgc ggctcggacg gtttcaccgt caatgccgtg
     5581 cagatcggct ccgacgagac gcgggagctg tatgccgtga gcgccgtcga tggtctgccg
     5641 gtcacggtct cgctcgacga ggccgtccaa gaagccgccg aggcggcgct cacggacgtc
     5701 ggcgacaagg gcggggcaag cgcactcgtg gcgatccggc catccgacat gcacgtccta
     5761 gcagcggcga gcggcgctgg ctcgcaaggg tattcgacgt cgacactggg gcagtaccca
     5821 cccggctcga ccttcaaggc cgtcaccgcg ctggccctgc tgcgctcggg cctgacgccc
     5881 aagaccacga tcgactgccc gaagaccgcg acagtcgagg gccgggcgtt caagaactac
     5941 gacgactatc cgccgaagcg gctgggctcc atcagcctca cgtcggccat cgccaactcg
     6001 tgcaataccg cattgatctc ccagctggac cggctcgacg gcgccgacct ggccgaggcc
     6061 gccgccgcgc tcggactcac ggcgcagccg cagctcgggg tgcccggggc gctcggcacg
     6121 gtgcccgccg acgccgccgg agtcgatctg gcggcttccc tgatcggcca gggccgggtc
     6181 gtgacgagcc cgatgggcat ggcgaccgtc gcggcgagca tcgcgcgggg ctcgatcggg
     6241 gcgccggtca tcgtcatcga cggccgagag ccgaagccct ctcccccatc aaaaccgttg
     6301 acccgcaagg agtccgagga gttgcaggcg ctcctgcggg ccgtcgtgac cgatgggtcg
     6361 gcgtccttcc tcaagggggt gcccggcgat gacgtgctgg ccaagaccgg caccgcggag
     6421 tatggcagcg gcgacccgcc accgacgcac gcctggatga tcggcatcca gggggatgtt
     6481 gccgtcgccg tcttcgtcga gaagggacag agcggcgcca agactgccgg gccgatcctc
     6541 gaggagttcc tgcgccggat gccgtgagcc gctcgggcgg tcaggtcgag accgcgtcga
     6601 cggccccgcc gtagcggcgc tcgcggttga catagagatc cacggcctgc cagaggtgcc
     6661 ggcggtcgaa gtcgggccac agggtgtcga gaaagaccat ctcggcgtag gcgctctgcc
     6721 acaacaggaa gttcgacgtg cgctgctcgc cgctggagcg gacgaagagg tcgacgtccg
     6781 gcatgtccgg ctcgggcagg tagcgggcga gcagcctctc gtcgaccccg cccggcttga
     6841 gccgcccggc agcgacctgc tcagcgattc gcttagcggc agaggcgatt tcggcgcgac
     6901 cgccgtagtt gacgcagaaa tagagggtga gcccggtgtt gtcctgcgtc agttcctggg
     6961 cgacctcgag ttccttgatg accgagcccc agaggcgggg gcgctggccg acccaccgca
     7021 tacggacccc ccactcgtgg agttggtcgc ggcggcggcg gatgacgtcc cgattgaagc
     7081 ccatgaggaa gcggacctcg tcggggctgc gcttccagtt ctcggtggag aaggcgtagg
     7141 cggagaggtg cgtcacgcct acctcgatgg ccccggccac cacgtcgagc agcgccgcct
     7201 cccccgcctc gtgccccttg gtgcgcggca ggccccgctg gttggcccag cgtccgttgc
     7261 cgtccatgac gacggcgacg tgcttgggca ggtccgcggc ggcataccgc ggcggcttgg
     7321 caccgctcgg gtgcgggaac ggcgggatca tgatcccgat cctgcaccca gagagagcga
     7381 ttcgttctga ggcggggcgg cgtgtggctc gcgctccacg tgcggcagcg agcgcagccc
     7441 ccgctcgagg tgccactgga cgtatgccgc ggtgatgccg ctgccctctc gccggtgttg
     7501 cgccgcactg gcatccgcga cgtcccagtc gccggcgagc agcgcggcga gcagatcgag
     7561 ggtcgcccgg gcaggggcga cggagcccgg ccggcggcac gacgggcata cggcaccgcc
     7621 ggcggcgaca ttgaaggcca gctgcggacc gggatcgccg cactgggcgc agtcgtggaa
     7681 gctcggcgcg tagccaccga tcgcgagcgc gcgcaggagg taggcgtcca ggacgagcga
     7741 ggcgtcgtgg gcgccgccgg cgagggcggc gagggctcct tggaggagga ggaactgctg
     7801 cagcgccggc gccttctcct cggtgagccg gtcggcggtc tccaggacgg tcgagagcgc
     7861 cgtgtaggcg cgatagtcgc gggcgatctg ctcgccgtgg gaggcgatcg agacggcctg
     7921 ggtgacggtg tcgaggttgc ggccctcgta acactggagg tcgacgacca tgccgggttc
     7981 caggcgggcg ccgaacctgc tcttggtgcg gcgcaccccc ttggcgacca cgcgcagctt
     8041 gccccgctcg cgcgccagca ccgtcacgat gcggtcggcc tcggcgagtt tgtgcgtgcg
     8101 cagcacgatc gcctggtcgc ggtaggtggg caccgaccca ttctcccccg ggacggccag
     8161 gctgccgtgc acggcgcgct cccggtatgt cggccgctca cctccggctg cacgcgccgc
     8221 ttgtctcgta cattcgttct attccggggt atggtggtgg gcgtgacctt gcaggatgtg
     8281 ctcgacttcg gtgacgggcc ggcggcggcg ggcgcgccgt tcgggccgct cgcgccggtg
     8341 cgcactcccc tgtcgtatgg cgcctgggtc gacgtcgccc cgggctggct gcgtgcggcg
     8401 gacgagctct tcgaggccct ccgcgccgac gtgccgtggc aggccgagcg gcgccagatg
     8461 tacgacaacg tcgtcgacgt gccgcggctg acccgcttct acggtgagtc cgaggtgtgg
     8521 ccacatgtcc tccttcccga gatctgccgc gccatcagcg atcactacct gcccacgctc
     8581 ggcgagccgc tcgtcaccgc cggcctgtgc ctctaccgcg acggcgccga ttccgttgcc
     8641 tggcacgggg atcggatcgg gcgctcccga ttccaggaca ccatcgtcgc catcctctcc
     8701 ctcggttcga cgcgcacgct cgcgctgcgc ccgcggggcg ggggcacgtc catacggctg
     8761 acctccggcc acggcgacct gatcgtgatg ggcggcagct gccagcgcac ctgggagcac
     8821 gccataccca agacccgcga gcccgtcggt ccccggatgt ctgtgcagtt ccggccggcc
     8881 ggcgtgcgct gacccacccg gcagggtgat caccgccact taggccattc ccgcgagtgt
     8941 ggcgccatcg agccgatgac gccacactct cgaaccgctt gccgtcaggg accgccgccg
     9001 ggacgggaga cggcgagttc gtccttcgtg gcggcctgat cgcgttcggc ccggttgaga
     9061 gcggacatga tcgcgcgcaa cgaggccttg acgatggact cgtggaggcc gacgccccac
     9121 aggacccgtt ctcccacagc gcattccacg tatgccgcgg cccgggcgtc gccaccggcc
     9181 gacagggcgt gctcgtggta gtcgagcacc cggacgtcga cgtcgagggc ggacatggcg
     9241 tcgatgaagg cgtcgatcgg gccattgccg ctgccggaga tgtgcacctc ggcgccgttg
     9301 tcgaggatgg ccgactcgat gtggtcgtcg ccgacgtcgg tgctcgtgag gatcgagcgg
     9361 accgggaaga agcggcccca gcgggtctgc gggttgtcgg agtggaggta ttcgtcggcg
     9421 aacatctccc agatctccgc cgccgtcagc tcgccgccct cggcgtcggt gcgccgctgc
     9481 accacctggc tgaactcgac ctggagccgg cgcggcaggt cgaggccgtg ctcggacttc
     9541 agcaggtagg acacgccgcc cttgccggac tggctgttga cgcgcaccac ggcctcgtag
     9601 gagcggccga tgtcgtgcgg gtcgatcggc aggtaaggca ctccccagtc gatgccgttg
     9661 acgtcggtgc cagcggccgc ggagcgcctg tccatgtcct cgaagccctt cttgatggcg
     9721 tcctgatggg agccggagaa ggcggtgtag accaggtcgc cgccccacgg gtggcgctcg
     9781 tggacggaca actggttgca gtgctcgacg gtgcggcgga tgtcggccat gtcggagaag
     9841 tcgatctgcg ggtcgatgcc ctgggagtag aggttcatcc ccagggtgac caggcagacg
     9901 ttgccggtgc gctcgccatt gccgaacagg cacccctcga tgcggtcggc accggcgaga
     9961 tatcccaact ccgcggccgc cacgccctcg ccccggtcgt tgtgcgggtg caagctcacc
    10021 accaccgagt cgcggcggtc gaggtgacgc accatccatt cgatcgaatc ggcatacaca
    10081 ttgggcgtgg ccatctccac cgtcgccggc aggttgacga tcatcttgtg gtcgggcgtg
    10141 gggtcgatga cctcgatgac ggcgttgcag atctcgaccg cgaactccag ctccgtgccg
    10201 gtataggact ccggtgagta ttcgtagtag acggtcgtct ccgggatcgt ctcctccagc
    10261 ttgcggcaca gccgcgcccc ctgcagggcg atgtcgatga tgccctcctt ggacatcccg
    10321 aagacgaccc gccgttgtag cacgctggtg gagttgtaga agtgcacgat cgcctgcttc
    10381 gacccgcgga tggcgtcata ggtccgctcg atgaggtggt cgcggcactg ggtgagcacc
    10441 tggatggtca catcgtcggg gatgtgcccg ccctcgatca gctcgcggac gaagtcgaag
    10501 tcggtctggc tggcgctggg gaagccgacc tcgatctcct tgtagcccat cttgaccagg
    10561 agcttgaaca tccgcagctt gcgctcggcg tccatcgggt cgatgagggc ctggttgccg
    10621 tcgcgcaggt cgaccgcgca ccaacgcggc gcctgcgtca tgaccttgtc gggccaggtg
    10681 cggtcgggca gttcgacctg gatctgctgc tggaagggga ggtacttcgt gaacggcatc
    10741 ggggtgggat gctgggggtg gatcatcgct tcggttcgcc ttcgtctcgg atcggagtgt
    10801 gcggcggcct tggggcacag cggactccgc gacgaggggc ggccgttgtc aggcctcgcc
    10861 gcggcagcga aggaggaggc gtccacgcat agtgcccaca gtatgccgcg tgcccccgcg
    10921 ccacgcgagg gcgggtccga actgtggtcg cgtcatacgc gcacccccaa cacctacggc
    10981 aagcgcccgt cgctgtccgg cagggctacc acgggtgtgc cgcccgcgcg ggccacgttg
    11041 gcaagatccg ggtcagcggt ggccagggcc gcgtcggtgg cggacgccgt cgcggccgcg
    11101 acacagtcgg ccatactcac cggacaacgc ttgctgccgt agttcgccgc gcgggtcaga
    11161 cccgccgacg ttgcgacggc ggcatccacg ggaatcaccc gcatgccggt cagttggagc
    11221 agcgccagat ccgcgagcac gtcatcctcc ggcctgccga aaacgcggac caactggtcg
    11281 accacttcag cgagattcac cgagttgatc gcacacgact catcctcgag gagccggcgc
    11341 acctcggagg ccacttcgtc gccgcgtaac agcccgatca cggcgtacgc atcaagcacg
    11401 atcaccgacg cgaccggtca cggtccgcct cgcgtgcccg ctcatccgca cgagccgatc
    11461 gcgtggccgg gccagggccc gcgtgggcgc ccgagacggc agcgaggatg tcgtcaggca
    11521 ccggccggac gatgacgtag tcacctcgat cgacgacgac gacccgctcg gcgtgccagc
    11581 ggcgacgaac ctcggcaggg agggagacct ggccgttccg cgagatcttc gccgtcgatg
    11641 gactcatgag gtcattgtat gctggcgccg ctccatacaa cggatcaccc gaattgttcg
    11701 aaggtggcgg tgaagtgccg cagggccggg ggctcgtcga cgatgcggta gccgggcagg
    11761 tcgccggccc gtgccttgac ccgctcgcag acctcgatga cgtagtcgat gtggctctgg
    11821 gtgtaggtgc ggcgcgggat cgccaggcgc accaactcca tcgccgccgg cgactcgctg
    11881 ccgtcggggt ggcggccgaa catcaccgac ccgatctcgc acgagcgcac gccccccgcc
    11941 tcgtacagcg cgaccgccag tgcctggccg gggaagtgca gcggctcgat gtgcggcagc
    12001 agcgcccggg catcgatata gacggcatgc ccgccggcgg gcttcaggca gggcatgccc
    12061 agcttgtcga gcgcgtcggc cagataggcc gtcgaccgga tgcggtagcg caggtagtcg
    12121 tgatcgacac actccttgag cccctgagcc agcgcctcga ggtcccggcc ggccagaccg
    12181 ccatatgtcg ggaaaccctc ggtcaggatc agcaccgacc ggcactgctg ggcgagatcg
    12241 tcgtcattga gcgcgagcca gcccccgata ttgcccatcg ggtccttctt cgccgacatc
    12301 gtcatcccgt cggcacaggc cgccatgtcg cggatgatgt cgggtacgtc gcgctcaccc
    12361 tggccctcct cacgctcccg gatgaaccac gcgttctccc cgaaccggca cgcgtcgagg
    12421 aagagcggtt tgccgtgcgc gtgggcgatc tcgctgaccg cacggaggtt ctccagcgaa
    12481 accggttgtc ccccaccgga attgttggtg atcgtcatca tcacgcaggg cacgttgtcg
    12541 ccgcgttcgg cgagcagggc ctcgagcctg gcggtgtcca tattgccctt gaaggggtgc
    12601 agcgacgacg ggtcgtgccc ttcggcgata acgagatcga gagcctcggc accggtgaac
    12661 tcgatgttcg cgcgcgtggt gtcgaagtgg gtgttgctcg gcacgacttt gccgacgccc
    12721 ccgagagcgc cgaagaggat cttctccgcg gcccggccct ggtgggtcgg gatgacgtgt
    12781 tcgtagggga agagttcgcg cacggcgtcg cggaagacga agtagctcgg cgaaccggca
    12841 tacgactcgt cgccgtgctg gatcgcggcc cactggtcgc gactcatcgc gcccgtgccg
    12901 gagtcggtga gcaggtcgat cagcacgttc tcggaccgga tctggaagag gttgtagccg
    12961 gcctcgcgcg ccacctgccg gcggtgctcc gccgtggtca ttcgcagggg ttcgaccgag
    13021 tggatgcgaa agggctcgat gacggtccgg aaagcttcgt cgctcatgcc cctcaccgta
    13081 tccagtggct caccgcaggc gcacggacag gcaggtcacg cacccctcga gtttctcgaa
    13141 ctcactgatg tccaccgtgg tgaccgcata gccgcgggca cggaggagtt ctgcgctgcg
    13201 tggcgcgctc gcggccatga gcagctgatc ctcgccgagg atgacgacgt gcgcgccgcc
    13261 ctcctcgggc atcgcttcgt atgccgggaa ggcctccttg tcgtccacca ccggatccca
    13321 tccgatgacc gtgccgtcgg gcagggcggt caccgccgac ttcaggtgca aggcgcgctc
    13381 gaccggcacg gcttgcgtgc gaacaccgaa ctcacgcagg tgtgtcgcga gctgttggat
    13441 ccccgcgccg ttggtgcggg acgagagccc gacgtatgcc gtgtctccgc tccgcagcac
    13501 gtcaccgccg tcgagggtgc cgggcgccgc gatgcgggcg atccggtaac cgagatcacg
    13561 catggtctgc tcaacgccga tcgtctcccg acgtcgctgc gccgcacccg gattcgcgat
    13621 gaccgccagg tcctcgtgaa cgacgacggg gtcctcgacg aagaccgcgt cgggacagtc
    13681 gtcggccggg ggcacctcga gcgtctccca cccggcttcc cgcagggcgt gcgcgtaggc
    13741 ctcccactga ccgatcgcca gctcgccgtc cacgggatcg cgctcgatat gggtgagcag
    13801 tccatgggca agccgcggcc cgggccgacg gatgagtgcc cgacgggaag tcatgcccac
    13861 cagcgtagga cgcctcagaa gtaggacttc tcgccgtcga gaagttcgcg gatgaggtcg
    13921 aggtgtccgg cgtgccgggc cgtctcctcg aacatgtggt ggatcatcca ccccaacgtc
    13981 gctcccgcgg ccgggaacgt cgtgccggca ccgcggtcgt cgagtccggc ggcggcgatg
    14041 acggcgtcgc tgcgccggca ctcctcctca tactccgcga cgagctggtc gagcggcttg
    14101 ccctgtccca caatgaattc gttgtcgtcg gggtcgtcat cgaacatcgg gttctcgctc
    14161 tcggccaccc cggagtaggc gacctggaac cacaggtgtt cgcaccagcg caggtgcgac
    14221 agcaggccgc cgacggtcat caggggcgag gtcgggatga ccacccgatg cacatctgcc
    14281 tcagccaaac cgtcggtctt gagaacgacg atctcgcgtt gcagctggaa ccagccgagc
    14341 agggaggtac ggtggtcggc gtcgaacgct gggcgcttca ggtcagccat ctgaccaatc
    14401 tggcaggaaa accgctggtt tcccaggtgc caggagtgca ctctggacga catgccgctc
    14461 ccacccgtgc ccgccggcct gatcgcccgc cccctggaag cgagcgacct cgacgccacc
    14521 tacgccgtct atgtcgccga cgaattcgtc aacaccggcc agcgcatggt cgagtgggag
    14581 gacatcgaat ccgactggca gcgtccgtct ttcgaccttt cgaccgattc ggtcggcatc
    14641 ttctacgggg accggctcgt cggtgcggcc gaggttttcg ccggccgccg ggccgacggg
    14701 gcggtcgcgc ccgattggca cggtcgcggc atcggcacct ggttgctggc ctggaccgag
    14761 caccgagcca aggagcaggg cagctccctc gtcggccaga gcaagttcgg tgggtcgccc
    14821 gccgaggccc tgctgcgggg ccgcggctac cacgagatgt ggacctcctg ggtcctcgaa
    14881 gtccccgagg accagacgat cacgccgcaa ccactcccgg atggctacgc actccgggag
    14941 ttcaccgccg cggacgagca cgcggcatac cacctgatcg aggatgcctt caacgagtgg
    15001 ccggaccgag acccggcgac cttcgaggac tggcgaccgc gcatcatcgg gcgagccggc
    15061 ttctccccgt ggcagattcg cttcgtgacc gacccgggcg ggaagccggt cggcgttgcc
    15121 gccatcatcc tcgacagtca gcagtgcggc tacgtcgacc aactcgccgt gcgcaaggac
    15181 caccgcggaa agggcctcgc ccgggcgctg ctggtcgacg ccttcgagga ggcccgccgc
    15241 cgcggtgcca cccgctccga actgtccacc gacagccgca ccggggccct ggggctctat
    15301 gagaaggtcg ggatggtcgt cacccagacc tggagacact gggcgaggga gctttaggcc
    15361 accccacgaa gtgtcctcgc tccgtcgtcg ctccggtact tcgcggggac cccgtctcgt
    15421 cggtggcttt tcgttagtcg ctggcttttc gttacacgtc cgaggccggc gagcgggctc
    15481 agggggcctg cggctcgctg atgtcagcca cctgagcctc gaagacccgc accaggtcgt
    15541 ccgccgccac acccagcgcc acgccgtgcc ggccggagcc catggacacg cggcggccgg
    15601 gaatggtcgc gtcgacgacg acgggccagc gcgtgctcga cccgaacggc gtgatcgtgc
    15661 cgcgctcgta gccggtggcg gccttggccg tcccggcatc gggcatcgac agccggctga
    15721 ccccgagaag ggcgcgcagc ttcggccatg agatctcccg gtctcccggc acgagcacga
    15781 acaggtagtc gtcgtcggcg cggcgaacca ccagcgtctt gacgatgtcg gcgggcatga
    15841 tgccccgcac cgcggcggcc tccgcgaggg agctcaccgg accgtgctcg gtcacggtgt
    15901 aatccaagcc caacgcttgg gcgtgcgcta tcgccggagt gctcggctca ctcacgtcgc
    15961 cggccacagc gagtgatagg cgttcaacgc cggttggcca cccagatggg catacaggac
    16021 agtcgaatcc gccgggatgt ccttggtgcg caccaactcg atgagaccgg cgagggactt
    16081 gccttcgtag accgggtcgg tgatcatcgc ctccagctgc gcccccatcg ccatggcctc
    16141 catggtcgac tcgacgggca cgccgtagag gtcgccggcc caaccctcga gcacctggat
    16201 ctcgtcgtcg cgcaggtcgc ggccgagttc gatcagctca gcggtatgcc gcgcgatccg
    16261 ctccacctgc tcgcgcgtct ggtccagggt cgccgaggcg tcgatgccga ggactcgccg
    16321 ccggatgccg gtcaggtcct cgagcgcagc gaagccggcg atcatcccgg cgtgggtcga
    16381 gcccgtcacc gtgcagacga tgatggtgtc gaagtgcacc cccaagctct tttcctgctc
    16441 ggccacttcg aacgcccagt tcgcgaaacc cagtcccccg agccggtgtt cggaggcgcc
    16501 cgcgggaatc ggatagggcg tgccgccgga ctcctcgacc tcgcgcagag cggccttcca
    16561 cgagtcgcgg atgccgatgt cgaagccgtg cgggtcgagc cgggcgtcgg cgtccatcat
    16621 ccgcgtcagg aggatgttgc cgaccttgtc gttgacgggg tcctcccacg gcacccattt
    16681 ctcttgcacg agacggcatt tcaggcccag gtgcgcagcg accgcggcca cctgacgggt
    16741 gtggttgctc tggtagccgc cgatggacac cagggtgtcg gcgccggact cgaggatgtc
    16801 ggggacgatg tattcgagct tgcggacctt gttgccgccg aaggccagtc cgctgctgac
    16861 atcttcccgc ttggcccaaa cctgtgctcc cccaagggca tccgagagcc gccgcagatg
    16921 atggaccgga ctggggccga aggtgagcgg atatcgcggg aagtccgaca gcttcatact
    16981 caggcccttc tgccatcgtg cgtcagcgtg cgccaggtct cgaactcgtg atcggacgct
    17041 gccagggcgt cccccgcggc acagagatgg atgaactccg catgccgggc gatcgagtcc
    17101 tgcaccgcga aggagtcgaa acgggctcgc tcgacccggc ggatcacggg cgtgaactgc
    17161 gcgagcacgg catcgatcgc gcgattgtcc gccaggacga cgggcacggc gtgcaggtcg
    17221 tcgtcggcgc gcagggcggc gtcgatgtcc ccgtccgcca gcgcggcggc atacctctcg
    17281 ctggcctgcg tcatcgcctc gacgtcctcc ggcgtcaggc gaccgacgga ttcgcgcacc
    17341 gcgagcgagc gcatcgaggc gacgacgtcg cgggcatggc gcacggccgt ggggtcgagg
    17401 tcggcgacga ccgtcgactt acccgggtcg gcgacgacca gcccggattg cgccagccgc
    17461 agcagggcct cacgcaccgg tgtccgggag acgccgagtc gagccgccag ctcttgatcg
    17521 ttgagtttct cccccggcgc gagcgagccg tcgacgatgg cgtcgcgcag ctgttcgtag
    17581 acgctctcgc gcagcagaga acgtcggctc ggaacggtgg cgtcagagag tggcatgtga
    17641 tatactgcat actgcccggc tagccggtgc gcaatatcgg gggatgcgga acgaccgagt
    17701 gaaccggggg tagctcggtc gttccgtgtg tctgccgagc acggctatca gggcgtctcg
    17761 ccgaccacga gttcgttgac cacctggatg tactcatcct cggcagcggc cgggtcggtg
    17821 ccgaccagac ccttccaggc gtcgtacttc gcccggccga cgaagtcggt gaagccgggc
    17881 cgcttgcccg acacatcccc ctcggtcgcc tgcttgtaga gcgcgtagag gcgcaacttg
    17941 gtgtcattgc cagggtcccg ggtcaggccc gggacggcgg cgactgcgga ttcgaaatcg
    18001 gtgatgtcag ccacgacaac aatctagctc gccggcccaa tccgccgtcg gagccactcg
    18061 gccgcgatct gcacggaatt ccgcccactt cgttgtggct tgggaacccg ccccggaggg
    18121 tgcagtcccc ccggggacac agggcccttg cgacgctatg tgcgtttctt gccggcatcg
    18181 ctgcgcttct cgcgccaact cccgtccttg ttgcggtcgc gttcctgctt cccgccaccc
    18241 ttcttcggga actgcgggac ctttccggca ttaccaggca tgctcgtcac ctcctttcgg
    18301 cagagttctg atcggcatgg ggcgttatgc cgccagacgt ggggccatgg cttccatctg
    18361 ctcgatgacc tgccggattg cctccggttg tctgtcgggc ggatagcggt acttcacaag
    18421 gagtcgcttg atcgaggacc gcaacttggc tcgcacgtca tcgcgaacgg tccagtcggt
    18481 cttgatatcc cgacgcatca cggccacgag ttcccgggcg atggtggcga gcacctcgtc
    18541 acccatgacc tcccgagccg actcgttgtc cgccaccgcg tcatagaacg ccagctg
//
LOCUS       DAOPJG010000045        18586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3141_length_18586_cov_8.071016, whole genome shotgun sequence.
ACCESSION   DAOPJG010000045 DAOPJG010000000
VERSION     DAOPJG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 18586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18586
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3141_length_18586_cov_8.071016"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..764
                     /locus_tag="PKU75_06135"
     CDS             45..764
                     /locus_tag="PKU75_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HPF80491.1"
                     /translation="MAKRSPEPAKRILIVVTSTDEYRDAGYRTGLWLGELTHFYDVVT
                     EAGHEVVIASTAGGRVPIDPESLGSLVVKMGDTDERYEDRAFMDLLSDTSRLRDVARE
                     DFDAIYLTGGHGTMFDFPGNADLAGILRRLDGQGRIISAVCHGPAGFLGATRADGKPF
                     LDGRKVTGFSWPEEKLAGRNKVVPFRLDERLKAEGASYTKALRPMASKVVTDGNLVTG
                     QNPASAEGVAKQVVKLLKKAQ"
     gene            complement(1072..1455)
                     /locus_tag="PKU75_06140"
     CDS             complement(1072..1455)
                     /locus_tag="PKU75_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPF80492.1"
                     /translation="MTIVVAYADTPPGRAALNLAAQEALHRKTSLIAVPAVSGTSLTE
                     SELTDRLGAAGAEIVAAGGTVSVGSSDLHDPADAVIQVAQQVDATLICLGLRHRSPVG
                     KLLLGSTAQRILLDATCPVLSVKAD"
     gene            complement(1484..2770)
                     /locus_tag="PKU75_06145"
     CDS             complement(1484..2770)
                     /locus_tag="PKU75_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPF80493.1"
                     /translation="MVGLGGGWMSGIKEWLTHWSQVIRGALIGIWAGVLPGVGATAGT
                     WLAYGQAVATAKDKRNFGKGDPRGIVGPESSNNSVEAGDLIPTLLFGIPGGVPSAMLL
                     GMLLTYGIQPGPAIVTEHLNLMYLIVWCFAIASILGAFVCFLATPSLAKLTKVPFAVL
                     GAGLLVIMLLGAFQEGGQIGDLWIMIILGAVGWLMKATDTPRAPFLIGFVLAIPMERY
                     YYLTDSLYDGLGWLTRPGVLVFLAVLIAPALWALVKRTRKSAPDLGHRGEEDLSHGEE
                     DETEDGQLKDSIWSLGLALGVTLIFGTALAVSQSFSAEAQLMPRLVSIGGLITGLILL
                     WKELRARRSIGDGPGWTHDVTIAMRMFAAMALFLVLVMLGGYLLAVALFVPAFLLFVA
                     RATPRTTIIYTLVLCAVLLTLPSILPVDLPTGLLQS"
     gene            complement(2834..3454)
                     /locus_tag="PKU75_06150"
     CDS             complement(2834..3454)
                     /locus_tag="PKU75_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF80494.1"
                     /translation="MTDSFAAGVRFVAAVGEAGEALGHYPRVALGAAYVDLKLSSPDA
                     IYRDDEGTEHIVEWVTQQDVDLARRIAEIAAAQGLTADPGAACVVELGLDAHDSATIA
                     PVWAALLAGEAEAQGRGTPSDEVRDATGRVPNLWFGDADGAEARQRFHVEVYLAADVA
                     AQRIAAALAAGGTVVDDSDAPGLTVIADQEGNRGVVCVAASPAPAG"
     gene            complement(3612..4157)
                     /locus_tag="PKU75_06155"
     CDS             complement(3612..4157)
                     /locus_tag="PKU75_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPF80495.1"
                     /translation="MRKATAHLFSSVDGIVERPDTFFQFESMGPEEEAFMAESLASCT
                     DVILGRKIYEEWAGYWPAQTDDPFAAFINPAPKYVASRTLTGDLAWENASVIDGDLLE
                     FVRSLKAGDGGDINVNGITVIRELVLAGLLDTLTLTVHPAAAGAGRTLFEGIDGPLLL
                     ELVDSKVTRTGNAILTYRTKA"
     gene            4260..4913
                     /locus_tag="PKU75_06160"
     CDS             4260..4913
                     /locus_tag="PKU75_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPF80496.1"
                     /translation="MRERDYGQYCGLARALDVVGDRWALLIVRDLLSGPHRFGELQAG
                     LPRIPSNVLTNRLKELEEAGVVERVVRPAPGRGTAYRLTTDGYALEETLVALGRWGAP
                     RLGDPREGEIVTAESFAMALRTTFRPDTAGDVDATTPVTYEVHLGDLVTHAIVTGDRV
                     TAGTGPATKADLIIRSGPEIKQVMAGEITPTQALRAGTVTIEGSRRLWTRFARTFRT"
     gene            complement(4931..5545)
                     /locus_tag="PKU75_06165"
     CDS             complement(4931..5545)
                     /locus_tag="PKU75_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80497.1"
                     /translation="MTIPHTHALPQPTHPARSGLPWTSDDYTTLVRLVREGRDLGEIC
                     AELERGESAVLDRTRRMLPLEERGGPRDHSIARLRMHLEKDPDYDWSTQMCAKPPPPV
                     YVPPIIKGIGGLADDDVVAVACLLADRPYAAPTSLHRRVFREVRTRGLRRTLEAQWIQ
                     GAQENLERVIDTDYDCYYGHPDYPPVYERDWPASYATPEPDYPW"
     gene            5701..7116
                     /locus_tag="PKU75_06170"
     CDS             5701..7116
                     /locus_tag="PKU75_06170"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HPF80498.1"
                     /translation="MGRRNQQRRAAKAKERRRHTVPAPAYTGAGPGCAALPPPANETE
                     QVRAETLEAIGHVATGRSAYAESGIQRLVTRSREPGHARVITRELASLLRELLRAAWM
                     AGWRPLDLTRHVERHLDARHVPILGDAMAAELGTYAAATLAPDWPGQLRAIDARRWWP
                     SDQDHVTARAAREGFEPVLRLALALAALLTRLPRIEMIDPAPGTAPPPSRTATRGVPA
                     GERLLERVRQLLAKAESTPYEAEADTFTTAAQSLMARHSIDAAMLAATTPHTDRPCAR
                     RVWIERPYEREKVRLLHVVAEANRCRTVWSSGLGFVTVVGHEVDQAAVDTLFTSLLLQ
                     GTRAMTHEGAASRSGAARTRGFRKSFLFSYAVRIGERLREATLAETEAAMGQEAAAMA
                     HQGAAPAPRPRGPGGAGRSQALVQVLSARSAEVDAAVDAMFPTTVARRSSVPSDLRGW
                     EAGWRAAEAATLAGATERLDA"
     gene            complement(7216..7449)
                     /locus_tag="PKU75_06175"
     CDS             complement(7216..7449)
                     /locus_tag="PKU75_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPF80499.1"
                     /translation="MSRAPEEIEVRDDTIRLGQLLKLAGLVDDGAAAREVIAAGEVTV
                     NGEVDTRRGRQVHPGDLVSVGGQEIRVVGPARR"
     gene            7560..8183
                     /locus_tag="PKU75_06180"
     CDS             7560..8183
                     /locus_tag="PKU75_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPF80500.1"
                     /translation="MSDDATELARAERHDLTMLLAGLGADDWARPTALPGWDVRDVAG
                     HVISYDVIGWAGFARRMARARFRLARANDVGVALARELSPAQIVGQFRAHPRPVGLTT
                     AFGGRLGLTDGLIHHQDIRRALDRPRVVPPERLAPALRFALWAPPLPGRRLARGLRLV
                     ATDLDVEWGQGSEVRGPAEALLMAVAGRAIAGELSGPGVPVLAARLG"
     gene            8286..8510
                     /locus_tag="PKU75_06185"
     CDS             8286..8510
                     /locus_tag="PKU75_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80501.1"
                     /translation="MGKNTVRDRLTRALADLDDAADPAGRLDAARRIRASAEELELDE
                     VSSARKQGTSWRAIGAVYGLTKQGAQQRFH"
     gene            complement(8468..9355)
                     /locus_tag="PKU75_06190"
     CDS             complement(8468..9355)
                     /locus_tag="PKU75_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPF80502.1"
                     /translation="MSPAVADQRRSQILVFLAAVVWAVGTAVGMGILGDGVSQQSRGL
                     FSDHATLIAPHGPAFSIWSVIYVGLLGYVIWQWLPRWSATPLAATTRVPAAWALALNG
                     IWLLVVQAGWVWVSVVVMVGLVVSLGILLDRVRFALAAAEIDMASVFAVPVTFGLYLG
                     WICVATCANIASALVGSGVEPHDRLATILTIVVLAVVVAIVFVLGRRFSSQAVVTALS
                     AAVVWGLAWIAVARFAGELRSTPVAWTALAAAALVALAWALRIRRRAEAKHARRRTPG
                     TAAVVCQWKRCCAPCLVNP"
     gene            9742..11154
                     /locus_tag="PKU75_06195"
     CDS             9742..11154
                     /locus_tag="PKU75_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPF80503.1"
                     /translation="MRTRLKVLTWVGIGVPVLVGAAGAAYATYYHDRALPGSSVVGID
                     VAGLTRDEVANALRAKADGVVIDLKLPDGHKQASLADLGTAVDVDATLARVFSANEDW
                     SSYAKALRESRPVAVVTTADRTKLSAFLTDLGESYARRPVDATVSLAKNKESFTVSPG
                     VAGRSLEATGLADIAAQAAASLTSSAATTSLVDREPEVTTAEAKALADKANAVVQTAA
                     EMTAGERTFAPSEAEKASWVTLPADVAAAPEVDAAKVTAWVAAHVKSIDTAPTSGMRY
                     LTSTGTLLRVITPASDGTQVTNSADVATSLTAALDGGKDTTATVKTKVLPATWTERTL
                     AAGAENLAYPAVAGEKWVDVNLSAHTMTPYVGGKAAASTVKMVHGAKDTPSDVGTFHI
                     YMKNPLMTMTGQNADGTDYETPDVPWSSFYNGGEALHGAYWRKTWGYAASHGCINLPI
                     PTAKWIYDWAPIGTPVVVHN"
     gene            11208..11552
                     /locus_tag="PKU75_06200"
     CDS             11208..11552
                     /locus_tag="PKU75_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPF80504.1"
                     /translation="MSEYEEAQPTRAEIDAREGPVVLAFGTNWCGYCLSAERFIAPAF
                     DAHPEVPLIKTEDGRGRPLGRSFRVKLWPTLIFLRDGAEVARVVRPTSRQVIDDAMHV
                     LVGPDAAATPTP"
     gene            11593..12135
                     /locus_tag="PKU75_06205"
     CDS             11593..12135
                     /locus_tag="PKU75_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80505.1"
                     /translation="MTAAAGLAAGCSGISPDHGQDTVEQRLTALRGVEPVDTRADLEF
                     PEAIDVTLAESATPDDVTALRAELAGLDEKSGLPKDWTTAKIRIKRPNGSDHPDELRS
                     AITCFTPEVDQHLRDWMSVGDEMTGAVVLFDVVADRAPRFVVLGDAATIEADKKAAAG
                     NPLVENATFRARGDRDDNWP"
     gene            12293..12502
                     /locus_tag="PKU75_06210"
                     /pseudo
     CDS             12293..12502
                     /locus_tag="PKU75_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            12968..13282
                     /locus_tag="PKU75_06215"
     CDS             12968..13282
                     /locus_tag="PKU75_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HPF80506.1"
                     /translation="MSDTEIPDRSLPITESAHGHDCGCHEVDEQLPELDARVIPHAIR
                     HGSVFGAVGQLRPGQAMVLVAPHNPLPLLKQLADREGDAIEVSYLQEGPDAWRLKLAR
                     VR"
     gene            13306..14397
                     /locus_tag="PKU75_06220"
     CDS             13306..14397
                     /locus_tag="PKU75_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80507.1"
                     /translation="MPAPSTALARLPFFLPAAISLLAGLYAASRLLLDEASTDRVAGA
                     HGILMVLGFLGTLIALERAVALREVWGYAAPAALGAGGLTLVVPVPRVISQVLLIVGC
                     ALATAVLAMLWRRARDDATATQVLAATLALGAAVLWTRLDVAGLLPWLVGYVVLTVCA
                     ERVELARLSLPPTAGPTLLALATAVHAGVVAATLWPAVGCRFLGGALIVLVAWLIRHD
                     VARRTIRRNGLPRYAAAAMLAGYFWLAVAGTMWLAGGQPASPQRYDVLVHACFLGFAM
                     SMVMAHAPVILPAVLRVKLPYRPILWLPLGLLHLGLALRVAAGLVLGHGLAWQASGFL
                     TVAALLALAGAAATCVIGGRTQRFQEVAA"
     gene            14394..17120
                     /locus_tag="PKU75_06225"
     CDS             14394..17120
                     /locus_tag="PKU75_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HPF80508.1"
                     /translation="MTIKTDEDEFVEAAPRVRKPKSTGRQGFWPWRDLPSLLWLIAVA
                     VVTFVHPFVERSRWLMVHLVLLGALTHAAMVWSTHFSQALLKTGPDVDGRRIQDRRAI
                     LLFAGVGVVVAGVLMDRWLVALAGAAGVSVAVFWHGLQLWRRLRASVAARFRVTIRYY
                     VAAALCVPVGATFGVLLARGPDDALKARLLLAHTGVLALGWIGLTVTGTLVTLWPTML
                     RSRMDDRAEALARGALPLFLGAIALIVGGALMGWMPVAGAGVLAWFASALWWGRALWG
                     PARRRPPREFGALSVAAALAWLAVLLVWTGVVLLRGHDWAELAEGHGRATAVLAAGFA
                     PQLLTGALSYLVPVVLGGGPNAVRTATDTLGRFGTTRLALINAGLILSLLPIPSIVRV
                     LTTTLVLAAYLCFLPLLAMAIRRSRNVVRAGGGLPTTPRGPVQPAGARSVWSSGQLVA
                     ALAVLGMAVALGVAADPGAAGFPVTSAGSAVPATGETTTVRVEAKGMRFIPDSVTVPA
                     GNRLVIDLTNTDPTTTHDLVLANGTRTGRLRPGRSGTLDAGVVGESLEGWCAVVGHRQ
                     MGMVFSVLVEGTPPVTAAGHGAHGPQQSAGAQSGSGAQAGSGAPVDLHGSFSPGFAAV
                     SPVLAPAPDATVHRLTLTAREVELEVAPGVWQKRWTFNGVTPGPTLRGKVGDRFVISL
                     VNDGTQGHSIDFHAGMLAPDRPMRTIPPGESLTYTFTATHSGIWMYHCSTMPMSVHIG
                     AGMHGAVIIDPPNLPQVDREYVLVQSEIFLGESRAKGFASEVAAEKVLAQAPDAVAFN
                     GIASQYDHHQLAATAGERVRIWVLDAGPNRPSSFHIVGGQFDTVFAEGAWLLGDPERG
                     GSGAGGSQVLALAPAQGGFVELEMPEAGHYPIVSHLMVDAERGAHGILDVTP"
     gene            17278..18585
                     /locus_tag="PKU75_06230"
     CDS             17278..18585
                     /locus_tag="PKU75_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPF80509.1"
                     /translation="MDSQTLINLGLVLVFVVIGGIFAATELALVSLRESQLSALERRG
                     ERGARVADVARDPNRFLAAVQIGVTVAGFLSAAYGGSTLAPDLAPYLERLGLGPGAAE
                     TTALVALTLLIAYLSLVLGELVPKRLALQKPAGIALAVAPVLDRFATLMRPVIWLLSL
                     STNAVVRLLGGDPHATADKVTEEELRELVSTHHELEEQERQILRDVFTVTETTLAEVM
                     KPRGDVTFLRGDIPLPEAAERVRTLPYSRYPVIGENFDDVLGFLHIRDLLGLPAADDR
                     TVSDVARPILMLPGTKLVLPSVATMRESETHLAIVVDEYGGTDGIVTLEDLVEEVIGD
                     IRDEYDAPESLRTARDLTRVDAGTTIADVEEELGISLPEGGYQTLGGLIFAELGRIPQ
                     AGESLTVGDTELQVVSMAGNRISEVAVRVIDPAEDTAAGSSPG"
BASE COUNT         2788 a         6503 c         6477 g         2818 t
ORIGIN      
        1 aacctcaaga agtttccgta ttcgcaccga gggagttgac acccatggcc aagcgttcgc
       61 ctgagcccgc caagcgcatc cttatcgtcg tcacgagcac ggacgagtac cgcgacgcgg
      121 gctaccgcac gggcctgtgg ctcggtgagc tcacgcactt ctacgacgtc gtcaccgaag
      181 ctggccacga ggtcgtcatc gcctcgaccg ccggcgggcg cgtgccgatc gatcccgaaa
      241 gcctcgggtc gctggtggtc aagatgggcg acaccgacga gcgctacgag gaccgggcct
      301 tcatggacct gctcagcgac acctcgaggc tgcgcgacgt cgcccgtgag gacttcgacg
      361 cgatctatct caccggcggg cacgggacga tgttcgactt ccccggcaac gccgacctgg
      421 ccgggatcct gcgccgcctc gacggccagg gccggatcat cagcgcggtc tgccacggcc
      481 ccgccggctt cctcggcgcc acgcgtgcgg acggaaagcc gttcctcgac ggacgcaagg
      541 tcaccggctt cagctggccg gaggagaaac tcgccggccg caacaaggtc gtccccttcc
      601 gactcgacga gcgactcaag gctgaaggcg cgtcatacac aaaagccttg cgccccatgg
      661 catccaaggt cgtcaccgac ggcaatctcg tgaccggaca gaacccggcg agcgccgagg
      721 gcgtcgccaa gcaggtcgtc aagctgctga agaaggcgca gtagctcgag gtgccgcggc
      781 gagcccgcat tccgggagga cgaggtcaat ccgcggcggt acacaccagc tacgagtgct
      841 cgcctcggca accgggccgc accgcatacc cgcaggtcac agggtcgcgc ggatccgccg
      901 gcccgactac tggtagctgg tgtgcacgcg tcggccgagt tcatccggac cgtcttcgcg
      961 ctgccgaagc tgaccggcac ggtgcccacg cgccttgcct ggagcaggca tgcggaatgc
     1021 cgcccggcgg acgtcgccgg gcggcattcg gagcaattac cgtcgaggcg ctcagtcggc
     1081 cttgacggac aacacggggc aggtggcgtc gagcaggatg cgctgtgccg tgctgccgag
     1141 gaggagcttg ccgacggggg atcggtgtcg cagcccgagg cagatgagcg tggcgtccac
     1201 ctgctgggcg acttggatga ccgcatccgc ggggtcgtgc aggtcggaac tcccgacgct
     1261 cacggtccct ccggcggcga cgatctccgc accggccgca ccgagccggt ccgtcaactc
     1321 cgactccgtg aggctggtcc cggacacggc ggggaccgcg atcagggacg tcttgcggtg
     1381 cagggcttcc tgggcggcga ggttgagggc cgcgcgccct ggtggggtgt ctgcgtaggc
     1441 cacgacgatc gtcatggttt tgtcctttca gtcagcttcg gattcagctt tgcaggaggc
     1501 cggtcggcag gtcgaccggc aggatcgagg gcagggtcag caggacggca cagagcacca
     1561 gcgtgtagat gatggtcgtg cgtggcgtcg cccgagccac gaagagcagg aaggccggca
     1621 cgaacagcgc gaccgcgagc aggtagccgc cgagcatcac gagcaccagg aacagcgcca
     1681 tggccgcgaa cattcgcatg gcgatcgtga cgtcatgggt ccatcctggg ccgtccccga
     1741 tggaacgcct cgcccgcaac tccttccaga gcaggatcag accggtgatg aggccgccga
     1801 tgctgaccag ccggggcatg agctgggctt ctgccgagaa cgactggctc accgccaacg
     1861 ccgtcccgaa gatgagcgtg acgccgagag cgagcccgag cgaccagatc gaatccttca
     1921 gctgcccgtc ctccgtttcg tcttcctcac cgtgcgagag gtcctcctcg cctcggtggc
     1981 cgaggtccgg ggcgctcttg cgggtgcgct tgaccagcgc ccagagggcc ggggcgatca
     2041 gtaccgcgag gaagacgagc acgcccggtc gggtcagcca gcccagaccg tcatacaggc
     2101 tgtcggtcag gtagtagtac cgctccatcg ggatagcgag cacgaagccg atcaggaacg
     2161 gggcgcgcgg ggtgtccgtc gccttcatca gccagccgac ggcgccgagg atgatcatga
     2221 tccacaggtc gccgatctgc ccgccctcct ggaaggcgcc gagcagcatg atgacgagca
     2281 ggccggcgcc gagcacggcg aaggggacct tggtcagctt ggccagactg ggcgtggcga
     2341 ggaagcacac gaaggcgccc aagatgctgg cgatggcgaa gcaccacacg atgaggtaca
     2401 tcaggttgag gtgttcggtg acgatggccg gaccaggctg gattccgtag gtcagcagca
     2461 tgccgaggag catcgcggag ggcacaccgc cgggtatgcc gaagagcagc gtcgggatga
     2521 ggtcgccggc ttcgacggag ttgttggaac tctccgggcc gacgatcccg cgggggtcgc
     2581 ccttgccgaa gttgcgcttg tccttggctg tcgcgacggc ctggccgtag gcgagccagg
     2641 tgccggcggt ggcgccgacg ccggggagca cgcccgccca gatgccgatg agggctccgc
     2701 ggatgacctg gctccagtgg gtcagccact ccttgatgcc ggacatccag ccaccgccca
     2761 gcccgaccat gtccttctga cagcgacgtc ggacacagcc cagacgtcag ggccccggct
     2821 cgtcgcggcc gggtcagccc gcgggcgccg gtgaggcggc gacgcagacg acgccccggt
     2881 tgccctcctg gtcggcgatc accgtcagcc ccggcgcgtc gctgtcgtcg acgacggtcc
     2941 cacccgccgc gagtgccgca gcgatccgct gcgcggccac gtcggcagcc aggtacacct
     3001 cgacatggaa gcgctggcgc gcctccgccc cgtcggcgtc gccgaaccac agattcggca
     3061 cccgtcccgt ggcgtcccgc acctcgtcgc tcggcgtccc gcgcccctgg gcctcggcct
     3121 cgccggcgag caacgctgcc catacggggg cgatcgtcgc ggagtcgtgc gcgtcgagac
     3181 ccagctcgac cacgcaggcc gcgccggggt cggccgtcag gccctgcgcg gcggcgatct
     3241 cggcgatgcg gcgcgcgaga tcgacatcct gctgggtcac ccactcgacg atgtgctcgg
     3301 tgccctcgtc gtcgcggtag atcgcgtcgg ggctgctgag cttgaggtcg acgtatgccg
     3361 cgcccagcgc tacgcgcggg tagtggccga gcgcctcgcc cgcctccccg acggcggcga
     3421 cgaagcggac gccggcagcg aatgagtcgg tcacatagcg ggtatgcagt ccctgggcca
     3481 gcttcgtcca gtcggtcagt ctggcctcgg cgatctgatc cccgtcagca tgtccatggc
     3541 tggtgacgat agcgatgaat cgtgtccgcc gcgccgggtg cgggcaagca gatccgcgac
     3601 cggcgctgcg gtcaggcctt ggtgcgatag gtgaggatgg cgttgccggt ccgggtgact
     3661 ttgctgtcca cgagttccag gagcagcggg ccgtcgatgc cttcgaagag ggtgcggccg
     3721 gcgccggccg cggcggggtg caccgtcagg gtaagggtgt cgagcagacc ggcgaggacc
     3781 agctcgcgga tgaccgtgat cccgttgacg ttgatgtcgc ccccgtcgcc ggccttcagg
     3841 cttcggacga actcgagcag gtcgccgtcg atgacggacg cgttctccca cgccaggtcg
     3901 ccggtcagtg tccggctcgc gacatacttc ggcgccgggt tgatgaaggc ggcgaacggg
     3961 tcgtccgtct gggccggcca gtagcccgcc cactcctcgt agatcttgcg tccgaggatc
     4021 acgtcggtgc aggacgcgag gctttcggcc atgaaggcct cctcctccgg tcccatgctc
     4081 tcgaactgga agaaggtgtc gggccgctcc acgatgccgt cgacggagct gaacagatgg
     4141 gcggtggcct tacgcatgcg ttcctcctgg tctgatgggt gtcgcactag gcgcgacatg
     4201 atcactctat ttaagcaagt aaaatctgta aagaaccatt ttcttgctaa gatgaagtca
     4261 tgcgggagcg tgactacgga cagtactgcg gactcgcccg ggctctcgac gttgtcgggg
     4321 accgctgggc gctgctcatc gtgcgtgacc tgctcagtgg gccgcaccgc ttcggtgagt
     4381 tgcaggctgg tttgccccgg atccccagca atgtgctgac caaccggctc aaggaactcg
     4441 aagaggcagg cgttgtcgag cgcgtggtgc ggccggcgcc ggggcggggg acggcatacc
     4501 gcctcacgac cgacggctac gcgctggagg aaacccttgt ggcactaggc cgttggggag
     4561 cgccacgcct cggcgatccg cgtgagggtg agatcgtgac agcggaatcc ttcgcgatgg
     4621 ccttgcggac gaccttccgt cccgataccg cgggcgacgt cgacgcgacc acacccgtca
     4681 cctacgaggt ccacctgggt gacctcgtca cgcacgccat cgtcacgggc gatcgcgtca
     4741 ccgcaggcac aggacctgcc acgaaggcgg acctcatcat ccggtccggc cccgagatca
     4801 agcaggtcat ggccggcgag atcacgccca ctcaagccct ccgcgcgggc acggtgacca
     4861 tcgagggatc acgcaggctt tggacccggt tcgcccggac cttccgcacc tgaccgatcc
     4921 tcgtggtgac tcaccaggga tagtccggct ccggggttgc gtagctcgcc ggccagtcac
     4981 gctcgtagac tggtgggtag tccggatgcc cgtagtagca gtcgtagtcg gtgtcgatca
     5041 cccgctccag gttctcttgg gcgccctgga tccactgcgc ctccagcgtg cgccgcaggc
     5101 ctctcgtgcg cacctcgcga aaaaccctgc ggtgcaggct ggtcggcgcg gcatacggcc
     5161 ggtcggccag cagacacgcg acagccacga cgtcgtcgtc ggccagccca ccaatgccct
     5221 tgatgatcgg cggcacatag accggcggtg gcggtttggc gcacatctgg gtgctccagt
     5281 cgtaatcggg atccttctcc aggtgcatgc ggagccgggc gatgctgtgg tctcggggac
     5341 caccgcgctc ctcgagaggc agcatccggc gcgtgcggtc gaggacggcc gattcgccgc
     5401 gctcgagctc ggcgcagatc tcgccgaggt cgcgcccctc gcggaccagg cgcaccaagg
     5461 tggtgtagtc gtccgaggtc cacgggagtc cggagcgcgc cggatgggtc ggctggggaa
     5521 gggcgtgcgt gtgaggaatc gtcatgcgga cagcctcccc ccacacggcg aaatgcggag
     5581 aaagttatcc acaagtgggc ggcggtgggc tcgggtgccg gccgcgttgt cggaggctcc
     5641 tggcagcttt gccaccagac gcgcaccagg ggggtgcgcc ggcggagaag gacgacgacg
     5701 atgggacgac gtaaccaaca gcggcgcgcg gccaaagcca aggagcggcg acggcatacc
     5761 gtgccagcac cggcatacac cggggccggc ccggggtgcg ccgcgttgcc accgcccgcg
     5821 aacgagaccg agcaggtgcg cgccgagaca ctggaagcca tcggacacgt cgccaccgga
     5881 cggtccgcgt atgccgagag cggcatccag cgactggtga cgcgctcgcg cgagcccggc
     5941 catgcccggg tgatcacccg ggagttggcc tcgttgcttc gggaactcct gcgcgcggcc
     6001 tggatggcgg gctggcgacc gctcgacctc acccggcacg tcgagcgcca cctcgatgcc
     6061 cgtcatgtgc cgatcctcgg cgacgcgatg gcggccgaac tcgggacgta tgccgcggcg
     6121 accctcgccc ccgactggcc agggcagttg cgcgccatcg atgcccgccg ctggtggccc
     6181 agtgaccagg accatgtcac cgcccgggcc gcgcgagagg ggttcgagcc ggtcctgcgg
     6241 ctcgctcttg ccctcgcggc actcctgacc cggctgccgc ggatcgagat gatcgatcct
     6301 gcgccgggca cggcaccgcc gccctcgcgg acggcgaccc gcggcgtacc cgccggtgaa
     6361 cgcctgctcg agcgggtgcg tcagctgctg gccaaggcgg agtcgacgcc ctatgaggcc
     6421 gaagccgata ccttcacgac cgccgcgcag tccctcatgg cccggcacag catcgacgcg
     6481 gccatgctcg ccgccaccac gccgcatacc gatcggccgt gcgcgcggcg agtgtggatc
     6541 gagcgaccct acgagcgcga gaaggtccgc ctgctccacg tcgtcgcgga ggcgaaccgg
     6601 tgccgcaccg tatggagttc gggcctcgga ttcgtcaccg tcgtcgggca tgaggtcgac
     6661 caggctgccg tcgacacgct gttcacttcc ctgctcctgc aagggactcg cgcgatgacg
     6721 cacgaagggg cggcctcgcg gtcgggagcg gcccggacgc gcggattccg caagtccttc
     6781 ctcttctcgt atgccgttcg catcggcgag cggttgcgcg aagcgaccct tgccgagacc
     6841 gaggcggcga tgggccagga ggcggcagcg atggcgcacc agggcgccgc tccggcgcct
     6901 cgccctcgag ggccgggcgg agctggccgc tcgcaggcgc tggtccaggt gctgtcggcc
     6961 cgctcggccg aggtcgacgc ggccgtcgat gcgatgtttc cgaccacggt cgcccggcgg
     7021 agttcggtgc cgagcgacct gcgcggctgg gaggcgggat ggcgcgcggc cgaagcggcg
     7081 acgctcgccg gcgccaccga gcggctcgac gcgtgacgcc gcgacctggg ccaccccacg
     7141 acgtgtcctc gctccttcgt cgctccggta cctcgcgggg aaccccgtcc cgtcggtggc
     7201 ttctcgttac gcgacctagc gccgtgccgg gccgacgacg cgaatctcct ggccaccgac
     7261 ggacacgagg tcccccgggt gcacctgccg gccgcgccgg gtgtcgacct ccccgttgac
     7321 ggtgacctca cctgccgcga tgacctcgcg ggcagcggcg ccgtcgtcga cgaggccggc
     7381 cagcttgagc aattggccga ggcgaatggt gtcgtcgcgg acctcgatct cctcgggtgc
     7441 tcggctcatg gcgggctagg ctaccgggcc gcggtcgggc catcgctcac ggcgccgccg
     7501 gcatcccgcc tgctgcgccg cgagtggcgc ggacccgtcg cggacgcgac gatgagctcg
     7561 tgagcgacga tgcgaccgag ttggcgagag ccgagcgtca cgatctgacg atgctgctcg
     7621 ccggtcttgg cgcggacgac tgggcccgcc cgacggccct tccgggctgg gacgtccgcg
     7681 atgtagccgg ccatgtcatc agctatgacg tgatcgggtg ggctgggttt gcgcgacgga
     7741 tggcacgggc tcggttccgc ctggcgcgcg ccaatgacgt cggcgtggcg ttggcgcgcg
     7801 agctctcgcc ggcgcagatc gtcggccagt tccgtgccca tccgcgaccc gtcggcctga
     7861 cgacggcctt cggtggtcgc ctcggcctga ccgatgggct gatccaccat caggacatcc
     7921 ggcgtgcgct ggaccggccg cgcgtcgtgc cccccgaacg gctcgcgccc gcactccggt
     7981 tcgccctctg ggcgccaccg ctgcccgggc gccggttggc ccgagggctg cgcctggtcg
     8041 cgaccgacct cgacgtcgag tggggtcagg gatccgaagt ccgcggtcct gccgaagcgc
     8101 tgctgatggc ggtggccggg cgagcgatcg cgggtgagct gtccggtccg ggcgtgccgg
     8161 tcctcgcggc ccggctcgga tgagcacctc cggcgcccag ccaggatccc tccccttcca
     8221 ggcaggccgg ccggcgatct ggcccgcccc ggacacatgg cgtgtcgcgc ccctaggctg
     8281 cgcccatggg caagaacacg gtgcgcgacc gcctcacccg ggcattggcc gacctcgacg
     8341 acgccgccga cccggccggc cggctggacg ccgctcgacg catccgagcc agcgcagagg
     8401 agttggaact ggacgaggtc agcagcgccc gcaagcaggg cacgtcctgg cgcgccatcg
     8461 gggccgtcta cgggttgacc aagcagggtg cacagcagcg cttccactga catacgactg
     8521 ccgccgtacc cggggttcgg cggcgtgcgt gcttggcctc agcgcgccgg cgaatgcgca
     8581 gcgcccacgc cagggcaacg agagccgcgg ccgcgagcgc cgtccacgcc accggggtcg
     8641 aacgcagctc tccggcgaac cgggccaccg cgatccaggc cagtccccag accacggcag
     8701 ccgacagggc cgtcacgacc gcttgactgc tgaaccgccg gcccaggacg aagacgatcg
     8761 ccaccacgac cgcgagcacg acgatcgtga ggatcgtggc cagacgatcg tgcggctcca
     8821 cccccgagcc gacgagggcg gaggcgatat tggcgcacgt ggccacgcag atccagccga
     8881 ggtagagccc gaacgtcacc ggcaccgcaa agactgaggc catgtcgatc tcggctgccg
     8941 cgagcgcgaa ccggacgcga tcgaggagga tcccgagcga aaccacgagc cccaccatga
     9001 ccacgacgga cacccacacc cacccggcct gcaccaccaa cagccagatc ccgttgagag
     9061 caagcgccca ggcggccggc acccgcgtgg tcgccgccaa gggcgtcgcc gaccaacgcg
     9121 gaagccattg ccagatgacg tacccgagca ggccgacata gatcacgctc cagatcgaga
     9181 acgccgggcc gtgcggcgcg atgagggtgg cgtggtcgga gaacagtccg cgggactgtt
     9241 gtgacacccc atctccgagg attcccatgc cgacagcggt gcccaccgcc cacacgacgg
     9301 cggcaaggaa gacaagaatc tggcttcggc gctgatcggc aacggctggc gacatgccgt
     9361 gaaccgtatg cggtggatcg cgacagaacg cccggggtga tcggccagtt cacggctgct
     9421 cgtgtgcgcg aacgttggag agtcagccga tgccccgcat tgccgcccct accgcgccag
     9481 gagagtgccg gcggcgtcgg tgccaccggt ctggatcgat gtcgtcaaga tcgacgccgt
     9541 ctggatcgat gtcggctgga tcggcattgt cgaggactaa atcgatttag gtcagagtgc
     9601 cagagagatc agccggtgtc aacggccttg ggaaaggccg gccgggattg agcggctgcg
     9661 gcgcgccccg cccggggtgt cgcatacttg gtagatgcat agggggcgaa tggccgcgaa
     9721 cgacacgagc gcgagcaacc cgtgaggaca cggctgaagg tactgacctg ggtgggcatc
     9781 ggagtgccgg tgctggtcgg cgccgcgggg gcggcctacg cgacctacta tcacgaccgg
     9841 gctctgcccg gcagcagcgt ggtcggcatc gacgtcgccg ggctgacccg cgacgaggtt
     9901 gcaaatgcat tgcgcgccaa ggcagatggc gtcgtcatcg accttaagct ccccgacggg
     9961 cacaagcagg cgtcgctggc cgacctcggc accgccgtcg atgtcgacgc caccctcgcc
    10021 cgggtgttct ccgccaacga ggactggtcg tcctacgcca aggcactgcg cgaatcccgc
    10081 ccggtcgccg tggtcaccac cgccgatcgc accaagctct cggcgttcct caccgacctg
    10141 ggcgagtcgt atgcccgtcg ccccgtcgac gccacggtga gcctggcgaa gaacaaggag
    10201 tccttcacgg tctcacccgg ggtcgccggc cggtccttgg aagccaccgg cctggcggac
    10261 atcgcggcgc aggcggcggc ctccctgacg tcgtcggcgg cgacgacctc cctggtggac
    10321 cgggagcctg aagtcaccac cgccgaggca aaggcgttgg cggacaaggc gaatgccgtg
    10381 gtgcagacgg ccgccgagat gaccgcgggc gagcggacct ttgcgcccag cgaggcggag
    10441 aaggcgagct gggtcactct gcccgccgac gtggccgccg ctcccgaggt ggacgccgcc
    10501 aaggtcaccg catgggttgc cgcgcacgtg aagtcgatcg acaccgcgcc gacgagcggg
    10561 atgcgttacc tgacgtcgac cggcacgttg ctgcgcgtga tcacacccgc gtcggacggg
    10621 acccaggtca ccaattcggc ggacgtcgcc acgtccctca ccgcggcgct ggacggcggc
    10681 aaggacacca ctgccacggt caagaccaag gtgctgccgg cgacctggac cgagcgcacg
    10741 ctggcggcgg gtgccgagaa cctcgcctat ccggccgtcg ccggcgagaa gtgggtcgac
    10801 gtcaacctgt cggcgcacac catgacgccg tatgtcggcg gcaaggccgc cgccagcacg
    10861 gtcaagatgg ttcacggtgc caaggacacg ccgtcggatg tcggcacctt ccacatctac
    10921 atgaagaacc cgctcatgac gatgaccggg cagaacgccg acggcaccga ctacgagacc
    10981 cccgacgtgc cgtggtcgtc cttctacaac ggcggcgagg cattgcacgg cgcctactgg
    11041 cgcaagacct gggggtatgc cgcgtcgcac ggctgcatca acctcccgat cccgacggcg
    11101 aagtggatct acgactgggc gccgatcggc actcccgtcg tcgtccacaa ctgatcccgc
    11161 tcgtccctct cgccccgatc agcgttcacc tcggtcatgc tctgcggatg agtgagtatg
    11221 aggaggcgca gccgacgcgc gcggagatcg atgcccgcga aggcccggtc gtgctggcgt
    11281 tcggcacgaa ctggtgcggt tattgccttt cagcagagcg attcatcgcg ccggcgttcg
    11341 acgctcatcc cgaggtgccg ctgatcaaga ccgaggacgg acgaggccgg ccgctgggtc
    11401 gctcgtttcg agtcaagctc tggccgacct tgatcttcct gcgcgacggc gcggaagtgg
    11461 cccgggtcgt gcggccgacc agccggcagg tcatcgacga cgcgatgcac gtgctcgtcg
    11521 gccccgacgc agctgcgacc ccgacgccat gatggccgct cgaggacata ggtggcgggt
    11581 ccggttcgcc atgtgacggc cgcggcgggt cttgctgcgg ggtgcagcgg gatcagcccc
    11641 gatcacggcc aggacaccgt cgagcagcgg ctcaccgcac tacgtggtgt cgagcccgtc
    11701 gacactcgag ccgatctcga gttccccgaa gccatcgatg tgactctcgc ggaatcggca
    11761 acgcccgacg acgtcaccgc ccttcgggcc gaactggccg ggttggacga gaagtcgggc
    11821 ttgccgaagg actggacgac cgcgaagatc cgaatcaaac gccccaacgg ttccgaccac
    11881 ccggacgaat tgcgcagcgc catcacctgt ttcacgcccg aggtcgatca gcacctgcgc
    11941 gactggatgt ctgtcgggga cgagatgacc ggcgccgtcg tcctgttcga cgtcgtcgcc
    12001 gaccgcgcac cgcgtttcgt cgtgctgggc gatgccgcaa ccatcgaagc ggacaagaag
    12061 gccgccgccg gcaacccact cgtcgagaac gccaccttcc gcgctcgtgg cgatcgcgac
    12121 gacaactggc cctgaccgga atcggagcga gccgacctgg gctcgagaga gtctggatga
    12181 cgcctcggac gccaccggcg tgccgagcct ttcgtcatgg acacgccttt ccccacgccc
    12241 tggccgtctc acgatcgcgg tccggctcgg atgatttgtt acggttagaa ccgtgaaaaa
    12301 ggggccgcta caacttgggc cggcgcccgt ccgcgaggcc gcgccgacgc ccgcacaggc
    12361 gctggtcctc gcggagctcg accgccgcgg cccggcgacc gtcggtgagg tcaccgcggc
    12421 cacggggcag cacggcaata cggtgcggga gcacctggac gccctcgtcg acctcggact
    12481 cgtcacccgc gcaccggtga gcgacggcgg gcgggggcga ccgtcatacc ggtatgccgt
    12541 ccgcccatct gccgcgcagc acccgctcag tgcgctgatc ggcgccctcg cgggcgtgct
    12601 ccgccagacg agcgatgttc cggtcgccgc ggcccggcgg ctgggtcgca ccagcggcgc
    12661 agcgcagccg aaggccctcg cacccggcgc cccgggagac ggcatcgacc ggctcgcgat
    12721 gctgttgacc gagcgcggat tcgatgcgac caggcgaccc gacggcgcgc tgcagttgcg
    12781 cacctgcccg ctgatcgacc tggcccgtgc cgaccccgag gtcgtctgcg gatttcacca
    12841 gggcctgctg gaccgtctcg ccgaacacct cgacctcgca ggcgcccgac tgcacgcctt
    12901 cgccgaaccc ggcgcctgca ccgtcacgct caacccccag aagaacccca accggaaagg
    12961 aaagcccatg tccgacaccg aaatccccga ccgcagcctc cccatcaccg agtccgccca
    13021 cggccacgat tgcggctgcc acgaggtcga cgagcagctg cccgaactcg acgcgcgcgt
    13081 cataccgcac gcgatccggc acggctccgt cttcggcgcc gtcggccagc tgcgccccgg
    13141 ccaggcgatg gtgctcgtgg cgccgcacaa cccgctgccg ctgctgaagc agctcgccga
    13201 ccgcgaaggc gacgcgatcg aggtgagcta cctgcaggag ggccccgacg cctggcgcct
    13261 gaagctcgcc cgcgtgcgct gacaccggac cacacggaat acccgatgcc tgcacccagc
    13321 acggctctcg cccgcttgcc gttcttcctc ccggcggcga tcagcctgct cgccggcttg
    13381 tatgccgcgt cccggctgct cctcgacgag gcaagcaccg accgggtggc cggtgcccac
    13441 ggcatcctca tggtgctcgg gttcctgggg acgctgatcg cgctggagcg ggccgtcgcg
    13501 ctccgggagg tctgggggta tgccgcgccg gccgctctcg gtgccggcgg gctgacgctg
    13561 gtggtgccgg tgccgcgggt gatctcgcag gtgctgctca tcgtcggctg cgccctcgcg
    13621 acggccgtgc tggcgatgtt gtggcggcgg gcgcgagacg acgccacggc gacgcaggtg
    13681 ctggcggcga ccttggctct cggggctgcg gtgctctgga cgcggctcga cgtggccggc
    13741 ctcctcccgt ggctggtcgg ctatgtcgtg ctcaccgtct gcgccgaacg agtcgagctc
    13801 gcccggctca gcctgccgcc gaccgccgga cccacgctgc tggcgctcgc cactgccgtg
    13861 cacgccggcg tggtggccgc gaccctctgg ccggccgtcg gctgccggtt cctcggcggc
    13921 gccctgatcg tgttggtggc gtggctgatt cgccacgacg tcgcgcgccg caccattcgc
    13981 cgaaacggtc tgccgcggta tgccgcggcc gccatgctcg ccggctactt ctggctcgcg
    14041 gtggccggca ccatgtggtt ggccggcggc cagccggcgt cgccccagcg gtatgacgtg
    14101 ctcgtccatg cctgcttcct cggcttcgcc atgtcgatgg tgatggcgca cgcgccggtc
    14161 atcctgccgg ccgtgctgcg ggtcaagctg ccgtaccggc cgatcctctg gctgcccctc
    14221 gggttgctgc acctcgggct ggcgctccgg gtggcggccg ggctggtcct cgggcacggc
    14281 ctggcgtggc aggcgtccgg gttcctcact gttgcagcgc tattggcgtt ggccggagcc
    14341 gcagcaacct gcgtcatcgg tgggcgcacg cagagatttc aggaagtagc agcatgacca
    14401 tcaagacgga tgaggacgag ttcgtcgaag cggccccccg tgtccgcaag ccgaaatcga
    14461 cagggcggca gggattttgg ccgtggcgcg atcttccgtc gctgctctgg ctgatcgcgg
    14521 tcgctgtggt gaccttcgtc catccgttcg tggagcgttc gcgctggctg atggtgcacc
    14581 tggttcttct cggtgcgctg acccacgcgg cgatggtgtg gagcacccat ttcagccagg
    14641 ccctgctcaa gaccggtccg gatgtcgacg gccgccgcat ccaggaccgg cgcgcgatcc
    14701 tcctcttcgc cggggttggc gtcgtcgtgg ccggagtgct gatggaccgc tggctggttg
    14761 ccctggccgg ggctgccggg gtgtcggtcg ccgtcttctg gcatggcctg cagttgtggc
    14821 gccggctgcg ggcctcggtg gccgcgcgtt tccgcgtgac gattcgctac tacgtcgcgg
    14881 cggcgctctg tgtgccggtt ggagcgacct tcggagtgct gctggcgcgc ggcccggacg
    14941 acgcactcaa ggcccggttg ttgttggcgc acaccggagt tcttgcgctc ggctggatcg
    15001 ggctgaccgt cacgggcacc ctcgtcaccc tgtggcccac catgctgcgc tcgcggatgg
    15061 acgaccgggc ggaggccctc gcccgcggcg cgctgcccct cttcctcggc gccatcgcac
    15121 tgattgtggg cggcgcactc atggggtgga tgcccgtcgc cggggccgga gtcctcgcct
    15181 ggttcgcctc cgcgctctgg tggggtcgcg ccctgtgggg gccggcgcgc cgccggccgc
    15241 ctcgggagtt cggtgcgctg tcggtcgcag ccgcgttggc gtggctcgcg gtcctgctcg
    15301 tatggaccgg tgtggtgctc ctgcgcggcc acgactgggc cgaactggcg gagggtcacg
    15361 gccgtgccac cgcggtgctc gccgccggct tcgcgccaca gttgctcacc ggcgcactgt
    15421 cctatctcgt gccggtcgtc ctcggcggcg gcccgaacgc ggtgcggacg gcgaccgaca
    15481 cgctggggcg cttcggcacc actcgactgg cgctgatcaa cgccggcctg atcctcagcc
    15541 tgctgcccat cccgtccatc gtccgggtgc tcacgacgac actcgtgctc gcggcatacc
    15601 tctgcttcct gcccttgctc gccatggcga tccgtcgctc tcgcaacgtt gtccgagcgg
    15661 ggggcgggct acccacgacc ccccgggggc cggtgcagcc ggcgggcgcg cggtcggtct
    15721 ggtccagcgg gcaactcgtc gccgcgttgg ccgtcctcgg catggcggtc gcgctcggcg
    15781 tcgcggcgga tcccggggcc gccggcttcc ccgtcaccag cgcggggagc gccgtgcccg
    15841 cgaccggaga gacgacgacg gtgcgggtcg aggcgaaggg gatgcgcttc atacccgact
    15901 cggtgaccgt gcccgcgggc aaccggctcg tgatcgacct gacgaacacc gacccgacga
    15961 cgacgcacga cctggtgctc gccaatggca cccgcaccgg gaggctgcgg ccgggccgca
    16021 gcggcaccct cgacgccggc gtcgtcggcg agagtctcga ggggtggtgc gcggtcgtcg
    16081 gccaccggca gatggggatg gtgttcagcg tcctcgtcga ggggacgccc ccggtcaccg
    16141 ccgctggtca cggcgcgcat ggaccgcagc aatccgctgg ggcacagtca ggttcgggag
    16201 cgcaggcagg ttcgggcgca cccgtcgacc tgcacggctc cttcagcccc ggattcgcgg
    16261 ccgtgtcacc cgtcctcgcg ccagcgcccg atgccacggt gcaccggctc accctgactg
    16321 cccgcgaggt cgaactcgag gtggcgccgg gcgtctggca gaagcgctgg acgttcaacg
    16381 gcgtgacacc cggcccaacc ctgcgcggca aggtcggcga ccggttcgtc atctcgctgg
    16441 tcaacgacgg cacgcaggga cactcgatcg acttccacgc ggggatgctc gcgccggacc
    16501 ggccgatgcg aaccatccca ccgggcgagt cactgaccta caccttcacc gccacccact
    16561 ccggcatctg gatgtaccac tgctcgacga tgccgatgtc ggtgcacatc ggagccggca
    16621 tgcacggcgc ggtcatcatc gacccgccga acctgccgca ggtcgaccgg gagtatgtgc
    16681 tggtccagtc ggagatcttc ctgggcgagt cgcgcgccaa gggattcgcg agcgaggtgg
    16741 cggccgagaa ggtgttggcg caggcgccgg atgccgtggc cttcaacgga atcgccagtc
    16801 agtacgacca ccatcagctc gcggcgacgg ccggcgaacg agtgcggatc tgggtgctgg
    16861 acgccggccc gaaccggccg tcctccttcc acatcgtcgg cggccagttc gacaccgtgt
    16921 tcgccgaggg cgcctggctg ctcggcgatc cggagcgcgg cggatcgggg gccggtggat
    16981 cgcaggtgct cgccctcgcg ccagcgcagg gcggcttcgt cgaactcgag atgcccgagg
    17041 ccgggcacta cccgatcgtc agccacctga tggtcgacgc cgaacgcggc gcccacggca
    17101 ttctcgacgt cactccgtag cctgccggtc ggccgggctt cgcacagggg tgacctgtgt
    17161 cgtcgcaggc tttggcgggg gtatcagcct gagagctcac gtatccttga gccccatgga
    17221 tgaccctcga agtccacgcc gttcactccc gactccgggt tgggggtgaa cgggccgatg
    17281 gacagccaga ccctgatcaa cctgggcctc gtgctcgtct tcgtggtcat cggcggcatc
    17341 ttcgccgcca ccgaattggc cctcgtctcg ctccgggagt cgcagctttc ggcattggag
    17401 cggcgcggcg agcggggtgc ccgggtggcg gacgtcgccc gcgatcccaa ccgcttcctc
    17461 gcagccgtgc agatcggggt gacggtcgcc gggttcctct ccgcggcata cggcggctcg
    17521 acgctggcgc ccgatctcgc gccctacctc gaacggctcg ggctcggacc gggagcggcc
    17581 gagaccaccg ccctcgtcgc gctgaccctg ctcatcgcct atctctccct ggtgttgggc
    17641 gagttggtgc ccaagcgtct ggctttgcag aagcccgcgg gtattgccct ggccgtcgcg
    17701 cccgtgctcg accggttcgc aacgctgatg cgaccggtga tctggctgct gtccctctcg
    17761 accaatgccg tcgtcaggct cctgggcggt gacccgcacg ccaccgcaga caaggtcacg
    17821 gaggaggaac tgcgcgagct cgtctccacc caccacgaac tggaggagca ggagcggcag
    17881 atcctgcgcg atgtcttcac cgtcaccgag acaacccttg ccgaggtgat gaaaccgcga
    17941 ggagatgtca ccttcctgcg cggcgacata ccgctgcccg aggctgccga acgcgtgcgg
    18001 accctgccct attcgcggta tcccgtgatc ggagagaact tcgacgacgt cctcggtttc
    18061 ctgcacattc gcgatctgct cggtctcccg gccgccgacg accgcacggt ctccgacgtc
    18121 gcgagaccga tcctgatgct tccgggcacc aagctggtcc tgccgtcggt tgcgaccatg
    18181 cgggagagcg aaacgcatct cgcgatcgtc gtcgacgagt atggcggcac cgacggcatc
    18241 gtgacgctcg aggatctcgt cgaggaggtc atcggcgaca ttcgtgatga gtatgacgcg
    18301 ccggagtcgc tacggaccgc ccgcgatctg acgagagtgg atgcggggac gacgatcgcg
    18361 gacgtcgagg aggagctggg catctcgttg cccgaagggg gctatcagac cctcggtggc
    18421 ctgatcttcg ccgagctcgg gcggatcccg caggccggcg aatctctcac ggtcggtgac
    18481 accgagctgc aggtggtgag catggccggc aaccgcatca gcgaagtcgc cgtgcgcgtg
    18541 atcgacccgg ccgaggacac ggccgccggc tcgtcgcccg gctgat
//
LOCUS       DAOPJG010000046        17774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3337_length_17774_cov_7.912410, whole genome shotgun sequence.
ACCESSION   DAOPJG010000046 DAOPJG010000000
VERSION     DAOPJG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17774
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3337_length_17774_cov_7.912410"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PKU75_06235"
     CDS             complement(<1..142)
                     /locus_tag="PKU75_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPF80510.1"
                     /translation="MPVERLMPTDESADLIDLVREITEKELRPRADASEAAAEKSDAF
                     PRD"
     gene            complement(206..1024)
                     /locus_tag="PKU75_06240"
     CDS             complement(206..1024)
                     /locus_tag="PKU75_06240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPF80511.1"
                     /translation="MTVRIWQRYVAIGDSFTEGMCDPEPEPPDGYAGWADRLAGHLSA
                     VAAEAEQPFDYANLAVRGRKLADVVGPQLDSALTMSPDLVSIVGGGNDILRPQVDIDG
                     IGEQLEAAVVRLREAGADVLLVTPTDTAKAGLFKAIRPRHAIHTANIHAMAARHGCFV
                     VSLWGMRSLQDWRMWAADRIHLTSEGHRRVALAAADALGFETDRADWTTPLPAAEALG
                     RREAFAGHAAWGKEHFGPWVNRHLHGRSSGDALAAKRPAPAPIPPARLTADPAR"
     gene            complement(1063..2538)
                     /gene="gtfA"
                     /locus_tag="PKU75_06245"
     CDS             complement(1063..2538)
                     /gene="gtfA"
                     /locus_tag="PKU75_06245"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HPF80512.1"
                     /translation="MPDSVELITYADRLGGDLPGLLDVLTGPLAGLFGGVHILPFYTP
                     FDGADAGFDPRDHTEVDPRLGTWADVAAIAKDHDVMVDVIVNHVSSTSPTFLDWLAKG
                     ADSAYDGMFLTLDGAFPDGATEADLTRIYRPRPGLPFTPYTTGDGSKRLVWTTFTPQQ
                     IDIDIHHPATRDYLLRILDTLARAGVTQVRMDAVGYAVKTPGTTSFMTPETFVFIDEM
                     AAWSRERGLEVLVEVHSYYQRQIEIAAQVDRVYDFALPPLILHALTAGDVEPLLAWMR
                     IRPLNAVTVLDTHDGIGVIDVGADADDRSRPGLLAPEQIDQLVERMHDNSGGTSRLAT
                     GAAASNVDLYQVNSTFYDALGQDDDRYLAARALQFWAPGRPQVYYVGLLAGANDVDLL
                     RRTSVGRDVNRHYYTREEIDSDLQRPVVRALFALIRLRNAHPAFDGEFEIDAAGSVLT
                     ATWTAGEEYARIDIDAASGEGTVMWTEDGAPRTSGLLALGG"
     gene            complement(2612..3286)
                     /locus_tag="PKU75_06250"
     CDS             complement(2612..3286)
                     /locus_tag="PKU75_06250"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPF80513.1"
                     /translation="MPRALADLIHPSWAAALEPVAGRIAELGDFLRAEVAAGRGYLPA
                     GPHVLRAFEQPLEAVRVLIVGQDPYPTPGHPIGLSFAVAPDVSPIPRSLANIYRELQT
                     DLALPPPSTGDLTPWADHGVLLLNRVLTVRPGASASHRGKGWEEVTDCAIAALVARGG
                     PLVAILWGRDARSLKPALGATPVIESAHPSPLSASSGFFGSRPFSRANAALVAMGADP
                     VDWSLP"
     gene            3349..4032
                     /locus_tag="PKU75_06255"
     CDS             3349..4032
                     /locus_tag="PKU75_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPF80514.1"
                     /translation="MTIPRWQGVLFDLDGTLVDSIPLIIASYDHALTSVLGQGRPEPE
                     VRGWIGRTLTSVFEQEYPERAAELESTYIAWNRANAATMIGRYDGVHDLLVGLSDAQA
                     RFGVVTSKRRATAREALVLAGADDLVDVLAASEDTSEHKPLPAPLLHGAARLSVPAEG
                     CAYVGDAVVDVQAAKAAGMTSIAVTWGAADAATLEAAAPDHLVHTAYELHDLLLGAAG
                     SGGDTQAMG"
     gene            4141..5052
                     /gene="cysD"
                     /locus_tag="PKU75_06260"
     CDS             4141..5052
                     /gene="cysD"
                     /locus_tag="PKU75_06260"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HPF80515.1"
                     /translation="MAQPGPATLTHLQRLEAESIHILREVVAEVENPVMLYSIGKDSA
                     TMLHLARKAFYPAPPPFPLLHVDTTWKFRAMYELRDKAAKEAGMELLVYQNPEALEKG
                     INPFDHGPLHTDMWKTEGLKQALDKYGFDAAFGGGRRDEEKSRAKERVFSFRNARHQW
                     DPKNQRPELWKLYNARKHKGESMRVFPLSNWTELDIWQYIHLEGIEIVPLYFAAERPV
                     VEFDGLTIMVDDDRMPLNGQTPVMKSVRFRTLGCYPLTGAVESQAHTLPEVIQEILLT
                     TTSERQGRAIDHDSAGSMEKKKQEGYF"
     gene            5052..6995
                     /gene="cysN"
                     /locus_tag="PKU75_06265"
     CDS             5052..6995
                     /gene="cysN"
                     /locus_tag="PKU75_06265"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HPF80516.1"
                     /translation="MTTTDATTTSAAATPDEPIYQVESLIAEDIDAYLEKHQHKTMLR
                     FITCGSVDDGKSTLIGRMLYDSKMIFEDHLAALEADSKKVGTQGEQIDFALLVDGLTA
                     EREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTAELAVILIDARKGVL
                     TQTRRHSYLTHLLGIRHIVLAVNKMDLVDYDQKVFDDIVADYRAFADSIGIGDFTAIP
                     ISGFKGDNITTNSENTPWYSGTTLMNFLETVELDQTLDQQRPFRMPVQRVTRPNLNFR
                     GFAGTIANGVLKPGDKVRVLPTNRTSTVARIVTFDGDLEQAVVGQAVTITLEDEIDCS
                     RGDVLVLDESPAETADHFECHLVWMDDAEMLPGRSYWLKIGTQTVSAQFERPKYQVNV
                     NTMEHVAAKTLGLNAIGVVTFSTDKPIVFEPYAQSHDLGGFIVVDKMSNATVAAGMVH
                     FALRRSANVGWQPLEIDRAHHARMKHQRPAVLWLTGLSGAGKSEVANELEVQLARMNR
                     HTFLLDGDNIRHGLSKDLGFDDADRVENARRLAEVAKLMADAGLITIVSSISPFRTER
                     DTARELIGDGEFMEIFVDMPLEEAEKRDAKGLYAQARAGELAHFTGVNSPYEAPTAPD
                     VRIDALTTSPKDAAAEIIARLIP"
     gene            7149..7820
                     /locus_tag="PKU75_06270"
     CDS             7149..7820
                     /locus_tag="PKU75_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HPF80517.1"
                     /translation="MRGLDETAYAEITTDLSERFADTVTPADAADAVAQARAELEPQS
                     RHPEFLGLLVAKRATDLLREHISSRGERLHAVPTVLFMCEHGAARSQMAAAVAHEFGG
                     DHLHVRATGRQQAAALDPTAAAVLHERGIDLDRLISAGPLADVVHAPDVLVVMGTDPA
                     GTLAHRRVDWEVPDPHGRPIEEVRAIADEIEDRVRGLLGDLSIPVAPARAGAAGATGT
                     PALTG"
     gene            7871..8875
                     /locus_tag="PKU75_06275"
     CDS             7871..8875
                     /locus_tag="PKU75_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPF80518.1"
                     /translation="MGFVERLDRAQRRFPPLGFPIAVAYKYFDDIGGYLSALLTYYAF
                     VSLFPLLLLLATALSFVLAGRPDLQRQILDSAVAQVPVLGDQLGDPRSLSGGASGVAI
                     GLAVSVYGALGVGNALQHAMNTVWAVPRNVRPNPVLARARSVVLVLTAGLAVLATTAL
                     SAFTAAATTWGPWTKVLSTVLTILVNAFIVLLVFRIGVRQGSIRKMLPGALLAAGAWQ
                     GLQTFGVVYVGRVVKNASTSNGVVAFILGLLAFTYLAAVILVLCAEVNVVRAERLYPR
                     ALLTPFTDDVDLTAADQETYADQARSMRTKGYERIDVTFEPEESGDAQGERGAAGSDP
                     "
     assembly_gap    8930..9029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9085..9930
                     /locus_tag="PKU75_06280"
     CDS             9085..9930
                     /locus_tag="PKU75_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF80519.1"
                     /translation="MASDNPEIGSSVQTGDILTNYHDLGTGDPLLLLHGSGPGVSAWA
                     NWRLPITRLDQHFRILAPDLAGFGYSVVPERFEYTRERWLAQIVAFLDALEVERTSVV
                     GNSFGGAMALSLAIAHPERVDKLVLMGSVGVPFEITPGLDAVWGYEPSIEKMGELMRE
                     AFAYNAALITDDMVRARYEASIRPGAQEQFAAMFPAPRQRWVDAMAHPEKDIRAIPHQ
                     TLVIHGRDDKVIPLSTTMTLLGWIEDSQAHIFGRCGHWTQIEHTDAFCREMTGFLLGI
                     SSAEA"
     gene            10028..11392
                     /locus_tag="PKU75_06285"
     CDS             10028..11392
                     /locus_tag="PKU75_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPF80520.1"
                     /translation="MIGTSYHANKHTELAALSAAVIDYAQERIRLAPPLDKPLTEAEL
                     IESVGQTITPGGLGGAEAMRIFAEDLAPTCLSTDHPRYLSFIPCAPSDAASLFDVVVG
                     ASSIYGGSWLEGAGAVYAENLALRWLADLAGMPATAGGVFTPGGTVGNLSALVAARHT
                     ARRTARDGVRPNILVATEGAHSSIKSAADVMDAEFAGVAVDSAGRLTGERLRPVLLER
                     GPENVFAVVATAGTTNFGIIDDLASVAEVCREFGIWFHVDGAYGGAGLAAPSIRDRYA
                     GIEHADSFIVDPHKWLFAPYDCCALLYRDPAEARAAHTQKAGYLDVLTEAPEWNPTDY
                     SIGLTRRARGLPFWFSLTVNGTDAYAAALEHTLSIARFAEEEVARRPYLELVRHRDLT
                     VIVFRRRGWEAADYHRWSDGMLADEFAFVVPTTHDGETLARFAIVNPETSIGDIRAIL
                     DTMA"
     gene            11653..11943
                     /locus_tag="PKU75_06290"
     CDS             11653..11943
                     /locus_tag="PKU75_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HPF80521.1"
                     /translation="MNTAAAVTRPPAAEALSDRDQQILAFERQWWKYSGSKEQAVRDL
                     FDMSATRYYQVLNGLLDNPAALAHDPMLVKRLRRLRASRQRQRSARRLGAEV"
     gene            complement(12012..12608)
                     /locus_tag="PKU75_06295"
     CDS             complement(12012..12608)
                     /locus_tag="PKU75_06295"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HPF80522.1"
                     /translation="MPALISEVLSWVRTGFPDGVPDAEAPALFAVLTERLGTERAQEV
                     LRQLSVDGRLTPQAASSLLADDVQKRQVAAKLVLGGWPLGPAEDAEPEPPQEGGALGR
                     IVAWLREGYPGGVPDHDYIPLLALLERRLTKSEVKQIARSLRRADVSPAGPEDIAAAI
                     TEYTLVEPRDADLRRVRNQLAKQGWPVDFPDPDQEPAH"
     gene            complement(12616..12912)
                     /locus_tag="PKU75_06300"
     CDS             complement(12616..12912)
                     /locus_tag="PKU75_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80523.1"
                     /translation="MNNLQFALEGAWKVLLVGVLLGSGLPAIFALGIRALAYGVGGDA
                     EVDHAPPHPVGKALAALCFAIVLGGVALGITIVAASGFGKAVSFEHVIPTIVDK"
     gene            complement(12909..14177)
                     /locus_tag="PKU75_06305"
     CDS             complement(12909..14177)
                     /locus_tag="PKU75_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPF80524.1"
                     /translation="MDTSVVILILVVVTALAFDFTNGFHDTGNAMATSIATGALKPKI
                     AVALSALLNLVGAFLSVEVALTVTNAVVNIQNKDGTPKEALLVDGGEHLLFIVLAGLV
                     GGIVWNLFTWLYGLPSSSSHALMGGLVGATIAGLGVSGVKWNGDGSKLDGVVGKVLLP
                     ALISPAIAIVVAAIGTWLIFKITAGVAQRFLNRGFRWGQIGSASLVSLAHGTNDAQKT
                     MGVITLALIAGGHWSDTEAIPLWVKLSAAAAIALGTYLGGWRIIRTLGKGLIEISPAQ
                     GMAAESSSAAVILTSSHLGFALSTTHVATGSILGSGVGRPGAQVRWHIAGRMLAAWAI
                     TLPSAGLVGALMYWLGNGIGGVAGAIIVFAILLGVAVWMWRRSRLTAVGAHNVNDDWE
                     PSVAPPEALPHHHAAAATTTTTTTTTEEKP"
     gene            14301..14747
                     /locus_tag="PKU75_06310"
     CDS             14301..14747
                     /locus_tag="PKU75_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80525.1"
                     /translation="MSEVDWSTPEGLAAITTYLGGRIPDWRAPVAHALALSPATSSPE
                     WEFAHVNAPGGRHALPAVILATVLGHDGSTATLEVSRSDLQEAVASLAPAAACTSLDH
                     PNLAAWRLALAEVESNPLRTMCAVFIADLADPVSSEADGSLRSRLV"
     gene            14919..16346
                     /locus_tag="PKU75_06315"
     CDS             14919..16346
                     /locus_tag="PKU75_06315"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPF80526.1"
                     /translation="MPRPHLTVSALALVGLLPTAPGLAAPAPAATPATAAVLRPGAPG
                     AGDPYFPDMGNGGYDVSSYDIALAWNPATKAIAATTTIKAVATQGLSRFNLDLKGLVV
                     QSVRVGGASAAYSRSGAQELVITPPKGIAKGASFTVAVTYKGVPKAINDPTLGRSGWV
                     ATADGAVALNQPFGAATYFPVNDTPRDKATYIHRITVPTGYQVIANGEPTAPMTSGGN
                     TTYVWKMTSPMASELSSVAIGRYTVTTGTVAGTSIRSISAIAKSIDRTGAGARLNTAT
                     GAVVNWQRNRFGAYPFASTGGLVAPIGVGYALEVQGRPVYDQGSPTVSTGLLAHELAH
                     QWFGNSVTPKQWKDIWLNEGFATYAEWLYDEAHGGPTAQQQFTAHYNSTSWTGKVADP
                     GRDHIYDGLVYTRGALTLHALRNRIGTATFDKLLRTWAATYRGRNVTTADFIATAERV
                     SGKNLDAFFQDWLYESGKPSLRSAQ"
     gene            16309..17376
                     /locus_tag="PKU75_06320"
     CDS             16309..17376
                     /locus_tag="PKU75_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPF80527.1"
                     /translation="MSPASPVSGPHNSFRDLGLRVGHVTRTGPGQRTGVTVVLVGAEG
                     ATAGVSVRGAAPGTRETDLLSPLCLVDRVHAIVLSGGSAFGLASADGVARALYADGIG
                     WPMGAPGEVVPIVPAAVIFDLGRGGDFGAFPSAADGAAAYAAASLGQVAGCGSVGAGT
                     GAQAGGLAGGLGSASAVLSDGTTVAALVVVNAIGSPVDPRTGQLYAVAHGLPGEFPDL
                     GSPGAGALAAYLRARHTELGESGLVPGGATTIGVIATDAALDKAQCAKLADVGHDGIA
                     RAIDPAHTMLDGDTLFALSTAARPAPDLGGLFALHEAAASCVTRAIGHALLTATSAAM
                     WPGTGQERLVASYRDAFALSG"
     gene            17540..>17774
                     /locus_tag="PKU75_06325"
     CDS             17540..>17774
                     /locus_tag="PKU75_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPF80528.1"
                     /translation="MAKIIAFDEEARRGLERGMNVLADAVRVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELDDPYEKIGAELVKEVAK"
BASE COUNT         2829 a         6213 c         6002 g         2630 t
ORIGIN      
        1 cgtcacgcgg gaaggcgtcg ctcttttccg cggccgcctc ggaagcgtcg gcccgcggcc
       61 gcagctcctt ctcggtgatc tctcggacca gatcgatcag gtcggcactc tcgtcggtgg
      121 gcatcaggcg ttcgacgggc atacccgcag cctacccagc ggtatctccg acgtttggcg
      181 ggtcatagcc cgccagatgt cggacttagc gggcggggtc ggcggtgagg cgggcgggcg
      241 gtatgggtgc cggagcgggg cgcttggcgg cgagggcgtc gccggaggag cggccgtgca
      301 ggtgccggtt cacccagggg ccgaagtgct ccttgcccca ggccgcgtgg ccggcgaagg
      361 cctcgcggcg accgagcgcc tcggccgccg gcagcggcgt cgtccagtcg gcgcgatcgg
      421 tctcgaagcc gagggcatcc gccgccgcca acgccacgcg ccggtgcccc tcgctggtga
      481 ggtggatgcg gtcggccgcc cacatccgcc agtcctgcag cgatcgcatg ccccacaggc
      541 tgacgacgaa gcagccgtgg cgcgccgcca ttgcgtggat attcgccgta tggatcgcgt
      601 gccgtgggcg aatcgccttg aacaggccgg ctttcgcggt gtcggtcggg gtgaccagga
      661 ggacatccgc gccggcctcc cgcaagcgca ccaccgccgc ttccaattgc tcaccgatgc
      721 cgtcgatgtc gacttgcggg cgcaggatgt cattgccgcc gccgacgatc gacacgaggt
      781 cgggcgacat cgtgagggcc gaatcaagct ggggcccaac gacatcggcg agcttgcggc
      841 cacgcaccgc gaggttggcg tagtcgaacg gctgctccgc ctcggcggcc acagcgctca
      901 ggtgaccggc gaggcggtcg gcccacccgg catacccgtc cggcggctcc ggctcgggat
      961 cacacatccc ctcggtgaag gaatcgccga tcgcgacata gcgctgccag atacgaacag
     1021 tcatgtctcg caaccctaat agcgccgcag ccggccgttc ggtcaccccc cgagtgccag
     1081 caagccactc gtgcgcggcg caccgtcctc ggtccacatc acggtgccct caccactggc
     1141 cgcgtcgatg tcgatgcgcg catactcctc cccggcggtc cacgtggcgg tgagcaccga
     1201 ccccgccgcg tcgatctcga actccccgtc gaacgccgga tgcgcgttgc gcaaccggat
     1261 caacgcgaac agcgcccgga cgaccggccg ctgcaggtcg gagtcgatct cctcacgcgt
     1321 gtagtagtgc cggttcacgt cgcggcccac ggaggtgcgc cgcagcaggt cgacatcatt
     1381 ggccccggcg agcagcccga cgtaatacac ctgcggtcgt ccgggcgccc agaactgcaa
     1441 agcccgcgcc gcgagatagc gatcgtcgtc ctggcccagc gcgtcataga acgtcgaatt
     1501 cacttgatag agatcgacat tcgacgccgc cgcccccgtc gccagccggg aggtgccgcc
     1561 gctgttgtcg tgcatccgct ccacgagctg gtcgatctgc tccggcgcca gcaggcccgg
     1621 ccggctgcgg tcgtcggcgt ccgctccgac gtcgatgacc ccgatgccgt cgtgggtgtc
     1681 gaggaccgtg acggcattga gcggccggat ccgcatccac gcgagcaacg gctcgacgtc
     1741 gccggccgtg agggcgtgca ggatcagcgg gggcagcgcg aagtcgtaga cccggtcgac
     1801 ctgagcggcg atctcgatct ggcgctgata gtaggagtgc acctcgacga gaacctcgag
     1861 cccgcgctca cgcgaccagg ccgccatctc gtcgatgaag acgaaggtct ccggtgtcat
     1921 gaacgacgtc gtgccgggag tcttgacggc atatccgacc gcgtccatcc gcacctgcgt
     1981 gacaccggcg cgggcaagag tgtcgaggat ccgcaacaga tagtcgcggg tcgccggatg
     2041 gtggatgtcg atgtcgatct gctgcggagt gaaggtcgtc cagaccaggc gcttggagcc
     2101 gtcgccggtc gtgtacggcg tgaacggcag ccccggccgc ggccggtaga tccgggtcag
     2161 gtccgcctct gtggccccgt cggggaaggc accgtcgagc gtgaggaaca tcccgtcgta
     2221 tgccgagtcg gcccccttgg ccagccaatc caggaaagtg ggggaggtgg aggagacgtg
     2281 gttgacgatg acgtccacca tgacgtcgtg gtccttggcg atcgccgcga catccgccca
     2341 cgtgcccagc cgcgggtcga cctcggtgtg gtcgcgcggg tcgaacccgg cgtccgcgcc
     2401 gtcgaacggg gtgtagaagg ggaggatgtg cacgccgccg aacagccccg ccaagggacc
     2461 ggtcaggacg tccaggagac cggggagatc acctccgagc cggtccgcgt aggtgatgag
     2521 ctcgacggaa tcgggcatgg cgaactcctt cggtcggatc cgaccaggct agccagcgct
     2581 gtcggacccg tccaccgctc ctgcgtcacg gtcagggaag agaccagtcg acgggatcgg
     2641 cgcccatggc caccagggct gcgttcgccc ggctgaacgg gcgcgagccg aagaatccgg
     2701 aactggccga cagcggcgac ggatgggcgc tctcgatgac cggcgtggcc ccgagtgccg
     2761 gcttgagcga gcgcgcgtca cggccccaga ggatcgcgac caacggcccg ccccgtgcga
     2821 cgagcgccgc gatcgcgcaa tcggtcacct cttcccaccc cttgccgcgg tgcgaggcgg
     2881 aggcgcccgg gcggacggtg aggactcggt tgagcagcag tacgccgtgg tccgcccacg
     2941 gggtcaggtc gccagtgctg ggcggcggca gggccaggtc ggtctgcagt tcgcgataga
     3001 tattcgccag gctgcgcggg atgggggaga catcgggggc gacggcgaag gacagcccga
     3061 tcgggtgacc cggcgtcggg taggggtcct ggccgacgat gagcactcgc accgcctcca
     3121 gaggctgctc gaaggcgcgc aacacgtgcg ggccggccgg cagatatccg cggcccgccg
     3181 ccacctcggc ccgcaagaaa tccccgagct cggcgatgcg gccggccacc ggctccaggg
     3241 ccgccgccca cgaggggtgt atgaggtcgg cgagcgcgcg cggcatacgt ccagcccttc
     3301 gtgtggtgaa ttggattctg tcctcccttg gctggcaggc tatcgagcgt gaccatcccc
     3361 cgctggcaag gcgtcctgtt cgatctcgac ggcacgctgg tcgactcgat ccccctcatc
     3421 atcgcctcct acgaccacgc cctgacgtcg gtgctcggcc agggccgccc ggagccggag
     3481 gtgcgcgggt ggatcgggcg gacgctgacc agcgtcttcg agcaggagta tcccgagcgg
     3541 gcggccgaac tcgagtcgac ctatatcgcc tggaaccgcg ccaacgccgc gacgatgatc
     3601 gggcggtatg acggcgtcca cgacttgctc gtcggcctca gtgacgctca ggcccgcttc
     3661 ggcgtggtca cctccaagcg gcgtgcgacg gcgcgcgagg cgctggtcct cgccggggcg
     3721 gatgacctcg tcgacgtgct cgccgccagc gaggacacca gcgagcacaa gccgttgccc
     3781 gccccgctgc tgcacggcgc ggcccggctg tccgtgccgg ccgagggctg cgcctatgtc
     3841 ggcgacgcgg tcgtcgacgt ccaggccgcg aaggcggcag ggatgacgag catcgccgtc
     3901 acatggggtg cggcggacgc cgcaacactc gaagccgcgg cgccggacca cctggtgcac
     3961 acggcatacg agctgcacga cctgctcctc ggcgccgcgg ggtccggcgg cgatacccaa
     4021 gcgatgggat agactcgtgg gtcgtgtgtg gcgtcgatgc tccctgacgt cgccgggcct
     4081 ccccgagcgg ctgccggggt ctggccgcac cgacttcact tgctcacgga aggctagttc
     4141 atggcgcaac ccggacccgc gacgctgact cacctgcagc gtctggaggc cgagagcatc
     4201 cacatcctgc gcgaggtcgt cgccgaggtc gagaacccgg tcatgctcta cagcatcggc
     4261 aaggactcgg cgacgatgtt gcacctggcg cgcaaggcgt tctatccggc gccgccgccg
     4321 ttcccgttgc tgcacgtcga cacgacctgg aagttccggg cgatgtacga actgcgcgac
     4381 aaggcggcca aggaggcggg catggagctg ctcgtctacc agaacccgga ggcgctcgag
     4441 aaggggatca accccttcga ccacgggcca ctgcacacgg acatgtggaa gacggagggg
     4501 ctgaagcagg ccctcgacaa gtacggcttc gacgccgcct tcggcggcgg ccgacgcgac
     4561 gaggagaagt cccgcgccaa ggagcgcgtc ttctccttcc gcaacgcccg ccaccagtgg
     4621 gaccccaaga accagcgccc tgagctgtgg aagctctaca acgcccgcaa gcacaagggc
     4681 gagtcgatgc gcgtcttccc gctgtcgaac tggaccgagc tggacatctg gcaatacatc
     4741 cacctcgagg gcatcgagat cgtgccgctg tacttcgccg ccgagcgccc ggtcgtcgag
     4801 ttcgacggcc tgacgatcat ggtcgacgac gaccggatgc cgctgaacgg acagacgccc
     4861 gtcatgaaga gcgtgcgctt ccgcaccctc ggctgctacc ccctcaccgg tgcggtcgag
     4921 tcccaggcgc acacgctgcc cgaggtcatc caggagatcc tgctgaccac cacctccgag
     4981 cgccagggcc gggcgatcga ccacgactcc gccggctcga tggagaagaa gaagcaggag
     5041 ggctacttct gatgacgacc acggacgcga ccaccacttc ggccgcagcg acgccggacg
     5101 agccgatcta tcaggtcgaa tccctcatcg ccgaggacat cgacgcctac ctcgagaagc
     5161 accagcacaa gacgatgctg cgcttcatca cctgtggctc ggtcgacgac ggcaagtcca
     5221 cgctcatcgg gcgcatgctc tatgacagca agatgatctt cgaggaccac ctcgccgcgc
     5281 tcgaggccga ctccaagaag gtcggcaccc agggcgagca gatcgacttc gccctcctcg
     5341 tcgacggcct cacggccgag cgtgaacagg gcatcaccat cgacgtcgcc tatcggttct
     5401 tcgcgaccga gaagcgcaag ttcatcgtcg ccgacactcc gggccacgag cagtacaccc
     5461 gcaacatggt cacgggcgcc tcgaccgccg agctggccgt catcctcatc gacgcgcgca
     5521 agggcgtgct cacccagacc cgccgccaca gttacctgac ccacctgctc ggcatccggc
     5581 acatcgtcct cgcggtcaac aagatggacc tggtcgacta cgaccagaag gtcttcgacg
     5641 acatcgtcgc cgactaccgc gcgttcgccg actccatcgg catcggcgac ttcaccgcca
     5701 tcccgatctc ggggttcaag ggcgacaaca tcaccaccaa ctccgagaac acgccgtggt
     5761 actccggcac gacgctgatg aacttcctcg agacggtcga actcgaccag accctcgacc
     5821 agcagcggcc gttccggatg ccggtgcaac gggtcacgcg tcccaacctc aacttccgcg
     5881 gtttcgccgg cacgatcgcc aacggcgtgc tcaagccggg tgacaaggtc cgcgtcctgc
     5941 cgaccaatcg cacgtccacc gtcgcccgga tcgtgacgtt cgacggcgac ctcgagcagg
     6001 ccgtcgtcgg gcaggccgtg accatcaccc tcgaggacga gatcgactgc tcgcgcggcg
     6061 acgtcctcgt gctcgacgag tcgcccgccg agacggccga ccacttcgag tgccacttgg
     6121 tgtggatgga tgacgccgag atgttgcccg gccggtccta ctggctcaag atcggcaccc
     6181 agaccgtcag cgcccagttc gagcggccga aataccaggt caacgtcaac accatggaac
     6241 acgtcgcggc gaagaccttg gggctcaacg ccatcggcgt ggtcaccttc tccaccgaca
     6301 agccgatcgt cttcgaaccg tatgcccagt cccacgacct gggtggcttc atcgtcgtcg
     6361 acaagatgtc caacgcgacg gtcgcggccg ggatggtcca cttcgccttg cggcgctcgg
     6421 cgaacgtcgg ctggcagccg ctggagatcg accgagccca ccacgcgcgg atgaagcacc
     6481 agcggccggc cgtcctgtgg ctgaccggcc tctccggtgc gggcaaatcc gaggtggcca
     6541 acgagctcga ggtgcagctc gcgcggatga atcgccacac gttcctgctc gacggtgaca
     6601 acatccgcca cggcctgtcc aaggatctgg gcttcgacga cgctgaccgg gtcgagaacg
     6661 cccgccgcct ggccgaggtc gccaagctga tggccgacgc cgggctgatc acgatcgtct
     6721 cctcgatctc gccgttccgc accgaacgcg acacggcgcg cgaactgatc ggtgacggcg
     6781 agttcatgga gatcttcgtc gacatgccgc tcgaggaggc cgagaagcgc gacgccaaag
     6841 ggctgtacgc ccaggcacgc gccggcgagc tggcgcactt caccggggtc aactccccgt
     6901 atgaggcgcc gacggcgccg gacgtgcgca tcgacgcgct gacgaccagc ccgaaggacg
     6961 ccgcggccga gatcatcgcc cgcctgatcc cctagcccga ctcaccgagg cgccgcagac
     7021 ccgaccgggc ttgcggcgcc tcgaaattct ccggcctccc atgccggtga accatgagag
     7081 acgtcaaggt gaacgaggag taaactgtct ggcacgccaa ggacggcgcg agcggatgga
     7141 ggcgcgggat gcgcggtctg gacgagacgg catacgcaga aatcacgacg gacctgagcg
     7201 agcgtttcgc cgacaccgtc actccggccg acgcggcgga cgccgtggcc caagcccgcg
     7261 ccgagctcga gccgcagtcg cggcaccccg agttcctcgg cctgctcgtg gccaagcgtg
     7321 caaccgacct gctgcgggag cacatcagtt cgcgcggtga gcgcttgcac gccgtcccca
     7381 ctgtgctgtt catgtgcgag cacggcgccg cacgctctca gatggccgcc gccgtggccc
     7441 acgagttcgg cggcgaccac ctgcatgtgc gagcgaccgg tcggcagcag gccgccgcgc
     7501 tcgatcccac cgccgccgcg gtcctgcacg aacgcgggat cgaccttgac cggctgatct
     7561 ccgctgggcc gctcgccgat gtcgtacacg ctccggacgt cctcgtcgtg atgggcaccg
     7621 acccggccgg cacgctggcg caccggcggg tcgactggga ggtgcccgac ccgcacggcc
     7681 ggccgatcga ggaggtccgc gccatcgccg acgagatcga ggaccgggtg cgtgggttgc
     7741 tcggcgacct gtccatcccg gtcgcgccgg cgcgggccgg cgcagccggg gccaccggca
     7801 cccccgccct gactggatag ccggcacggc agcaggcgca tgccggcgcg ggatgcgcga
     7861 tcatgcaccc atgggtttcg tcgaacggtt ggaccgcgcc cagcggcgat tcccgccgct
     7921 gggtttcccg atcgcggtgg cctacaaata cttcgacgac atcggtgggt acctgtcggc
     7981 actgctgacc tattacgcgt tcgtctcact gttccccctg ctcctgctgc tcgcgacggc
     8041 gctgagcttc gtgctggccg gccgtccgga cctgcagcgt cagatcctcg actcggccgt
     8101 ggcccaggtg cccgtgctcg gcgaccaact cggggatccg aggtccctga gcggcggcgc
     8161 atccggggtg gccatcggcc tggcagtgtc ggtctatggc gcgctcgggg tggggaacgc
     8221 gcttcagcac gcgatgaaca cggtgtgggc ggtgccgcgc aacgtgcggc ccaatcccgt
     8281 gttggcgcgg gcccgcagcg tcgtgctcgt gctgacggcc gggctcgctg tcttggcgac
     8341 cacggccctc tcggcgttca cggccgccgc cacgacctgg gggccatgga ccaaggttct
     8401 ctccacggtc ctcaccatcc tggtgaacgc cttcatcgtc ctgctcgtct tccgcatcgg
     8461 cgtgcggcag ggttccatcc gcaagatgct cccgggggcc ctgctcgcgg ctggtgcctg
     8521 gcaagggctg cagaccttcg gtgtggtgta tgtcggccgg gtcgtcaaga acgcgtcgac
     8581 gtccaacggc gtggtcgcgt ttatcctcgg ccttctcgcg ttcacctacc tggcagcagt
     8641 catcctggtg ctctgcgcag aggtcaatgt cgttcgggcg gaacggcttt acccgcgtgc
     8701 cctcttgact ccgttcaccg acgacgtgga cctgaccgcg gccgatcagg agacgtatgc
     8761 cgatcaggca cgcagcatgc gcaccaaggg ttacgagcgg atcgacgtga ccttcgagcc
     8821 ggaggagtcc ggcgacgccc agggagagcg aggcgccgcg gggtccgatc cctagcagcg
     8881 ccgccggcta tgccgcccga gggcgatgac ggtcacccgc actgagcccn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccggccacct cggcctcttc gcgcagagcg
     9061 ggccgttggg cataggctct gagaatggcc agcgacaacc cggagatcgg gagcagcgtc
     9121 cagaccggcg acatcctcac gaactatcac gatctaggca ccggggaccc gctgctgttg
     9181 ttgcacggct cagggccagg cgtgagcgcc tgggccaatt ggcgcctgcc gatcacccgc
     9241 ctcgaccagc acttccggat cctcgcaccc gacctggcgg gcttcggcta ttccgttgtg
     9301 ccagaaagat tcgaatacac gcgggagcgt tggctcgcac agatcgtcgc cttcctcgac
     9361 gcgctcgagg tggagaggac cagcgtggtc ggcaactcct tcggtggcgc gatggcgctc
     9421 tccctggcga tcgcgcatcc cgagcgggtg gacaagctcg ttctcatggg cagcgtcggg
     9481 gtgcccttcg agatcacgcc cgggctcgac gcggtgtggg ggtacgaacc atcaatcgag
     9541 aagatgggcg agttgatgcg cgaggcgttc gcgtacaacg cggcgctcat caccgacgac
     9601 atggtgcgcg cgcgctacga ggcgtcgatc cggcccggtg cacaggagca gttcgcggcc
     9661 atgttcccgg cgccgcgaca gcgctgggtg gacgcgatgg cccacccgga gaaggacatt
     9721 cgggcgatcc cccaccagac cctggtgatt cacggccgcg acgacaaggt gatccccctc
     9781 agcacgacca tgacgctgct ggggtggatc gaggacagcc aggcgcacat cttcggccgc
     9841 tgcggccact ggacgcagat cgagcacacc gacgccttct gtcgcgaaat gaccggcttc
     9901 ctgttgggaa tcagctcagc cgaggcctga gccgatcgag gcgttgggcg gcaggaccgc
     9961 gccgggaccg cgcgtgccag cgccgtaggg gacaccgctc accgtgtccg gcaagtatcc
    10021 tcacggcgtg atcgggacct cctaccacgc caacaagcac accgagctcg cggccctctc
    10081 cgccgcggtg atcgactacg cgcaggagcg catccggctg gcgccaccgt tggacaagcc
    10141 gctcaccgag gccgaactca tcgagtccgt cggacagacg atcacgcccg gaggtctcgg
    10201 cggagccgag gcgatgcgca tcttcgccga ggatctggcc cccacgtgcc tgtccaccga
    10261 ccacccgcga tatctctcct tcatcccgtg tgcgccgagc gatgcggcgt cgttgttcga
    10321 cgtcgttgtc ggggcctcgt ccatctatgg cggctcctgg ctcgagggcg ccggggccgt
    10381 gtatgccgag aacctcgccc tgcgctggct cgccgacctc gcaggtatgc cggcaaccgc
    10441 cggcggtgtc ttcacccccg ggggcacggt ggggaacctg tccgccctgg tggcagcacg
    10501 gcataccgcc cgccgcaccg cgcgagatgg ggtgcgcccc aacatcctcg tcgccaccga
    10561 gggcgcgcac agttcgatca aatccgccgc cgacgtcatg gacgccgagt tcgcgggtgt
    10621 ggccgtcgac tcggcgggcc ggctcacggg ggagcggttg cggcccgtcc tgctcgagcg
    10681 cggcccggag aacgtcttcg ccgtcgtcgc caccgccggc acgaccaact tcgggatcat
    10741 cgacgacctc gcctccgtgg ccgaggtgtg ccgcgagttc gggatctggt tccacgtcga
    10801 cggggcctac ggcggggccg gcctcgccgc accgtcgatc cgggaccgct atgccggcat
    10861 cgagcacgcc gactcgttca tcgtcgaccc gcacaagtgg ctcttcgcgc cctacgactg
    10921 ctgcgccctg ctctatcgcg atcccgccga ggcgcgcgca gcccacacgc agaaggcggg
    10981 ctacctcgac gtgctcaccg aggcgcccga gtggaatccc accgactact ccatcggcct
    11041 gacccgccgc gctcgtggcc tgccgttctg gttctccctc accgtcaacg gcaccgatgc
    11101 gtatgccgcg gcgctcgagc acacgctgag catcgcccgg ttcgccgagg aggaggtcgc
    11161 gcgccggccc tatctggaac tggtccgcca ccgggatctg accgtcatcg tcttccggcg
    11221 ccgcggctgg gaggcggccg actatcaccg gtggagcgac ggcatgctcg ccgacgagtt
    11281 cgctttcgtg gtgccgacca cgcacgacgg ggagacgctg gcgcgcttcg ccatcgtcaa
    11341 ccccgagacc tccatcggcg acatccgtgc catcctcgac accatggcct gattgccctg
    11401 ggggagaagg acgctgcgta tgtggtgacg ggtggcgcgg acggggatcg gtcgcgcgat
    11461 cgcggaacgg ctggccacgg atgaccccgt cgtcctggac acggcgggaa cggcccggat
    11521 ccggaggcgc gacactcgtc acccgggtga tcagtgctcg cgtggttcct ctccatccaa
    11581 gcgcccggaa aggcgaggaa cacgagcgcg gactgaatga catcggtgtg attccgccgt
    11641 atgctggcgt cgatgaacac cgccgccgcc gtcaccaggc cccccgcagc cgaggcgttg
    11701 tccgaccggg accaacagat tctcgctttc gagcggcagt ggtggaaata ctccggctcc
    11761 aaggagcagg cggtgcgcga cctattcgac atgagcgcga cgcgctacta ccaggtgctc
    11821 aacggcctgc tggacaaccc cgccgcactg gctcacgacc cgatgctcgt caaacgccta
    11881 cgccggctgc gggcctcccg gcagcggcag cgctcggccc ggcgcctggg cgcggaggtc
    11941 taggacccag cacagacgaa ggaacgcccg tccgtgtcgg ccccggacgg gcgttccttg
    12001 tcttgggcga ctcagtgcgc cggctcctgg tccgggtcgg ggaagtcgac cggccagccc
    12061 tgcttggcca actgattgcg caccctgcgc agatcggcgt cacgcggctc gaccagggtg
    12121 tattcggtga tcgctgcggc gatgtcctcg gggccggcgg ggctgacatc ggcccgccgg
    12181 agcgaccggg cgatctgctt gacctcggac ttggtcaggc ggcgctccag cagtgccagc
    12241 agcggaatgt agtcgtggtc gggcacccca ccggggtagc cctcgcgcag ccacgccacg
    12301 atgcggccca gggcaccacc ctcctgcggc ggctccggct cggcatcctc ggccgggccg
    12361 agcggccagc cgccgaggac gagtttggca gcgacctgtc gtttctgtac gtcgtcggcg
    12421 aggagagacg aggccgcctg aggggtgagc cggccgtcga ccgacagctg gcgcaggacc
    12481 tcctgcgccc gctcggtgcc gagccgctcg gtgagcacgg cgaacagggc cggcgcctcg
    12541 gcatcgggga caccgtcggg gaagccggtg cgaacccagc tcaggacctc ggaaatcagg
    12601 gcgggcatgg gttcctcact tgtcgacgat cgtcgggatg acgtgctcga agctgacggc
    12661 cttgccgaaa ccggatgcgg ccacgatggt gatgccgagg gcgacgccgc cgagcacgat
    12721 cgcgaagcac agcgcggcca gcgccttgcc gaccgggtgg ggcggggcgt ggtcgacttc
    12781 ggcgtcgcct ccgacaccat aggccagcgc ccggatcccg agggcgaaga tcgccggcag
    12841 cccggatccg aggaggacgc ccaccaggag taccttccac gcgccttcga gcgcgaactg
    12901 gaggttgttc acggcttctc ctcggtcgtg gtcgtggtcg ttgtcgtggt cgcagctgcg
    12961 gcgtggtggt gggggagggc ctcgggcggg gcgaccgagg gctcccagtc atcgttgacg
    13021 ttgtgggcgc cgacggcggt aagccgcgag cgccgccaca tccagacggc gacgcccaac
    13081 aggatcgcga agacgatgat ggcgccggcg accccgccga tcccgttgcc gagccagtac
    13141 atcaacgccc cgaccaggcc ggccgacggg agggtgatgg cccaggccgc gagcatgcgg
    13201 ccggcgatat gccagcgcac ctgggcgccc ggccggccca ctccggaccc gaggatcgag
    13261 ccggtggcga cgtgggtggt cgacaacgcg aaccccaggt ggctcgaggt gaggatgacg
    13321 gcggcggaac tggactccgc ggccataccc tgcgccggcg agatctcgat caggcctttt
    13381 ccgagggtgc ggatgatgcg ccagcccccg agataggtgc ccaaggcgat cgcggcggcg
    13441 gcggagagct tgacccacaa cgggatggcc tcggtgtcgc tccagtggcc gccggcgatc
    13501 agcgccaggg tgatgacgcc catcgtcttc tgcgcgtcgt tggtgccgtg cgcgagggag
    13561 acgagcgagg cgctgccgat ctgtccccag cggaaacccc tgttgaggaa gcgctgcgcg
    13621 acaccggcgg tgatcttgaa gatcagccag gtcccgatag cggcgacgac gatggcgatc
    13681 gccggcgaga tcagggccgg caggagtacc ttgcccacga ccccgtcgag cttgctgccg
    13741 tcgccgttcc acttcacacc cgagacgccc aagccggcga tcgtcgctcc gacgagcccg
    13801 cccatgagcg cgtgggagga ggaggacggc agcccgtaga gccaggtgaa caggttccag
    13861 acgattccgc cgaccagccc ggccagcacg atgaacagca ggtgttcgcc gccgtcgacg
    13921 agcagggctt ccttgggtgt gccgtccttg ttctggatgt tgacgacggc gtttgtcacc
    13981 gtgagggcga cttcgacgga caggaaggca ccgaccagat tcagcagcgc cgagagggcc
    14041 acggcgatct tcggcttcag ggcgccggtg gcgatcgagg tcgccatggc attgccggtg
    14101 tcgtgaaaac cattcgtgaa atcgaacgcg agagcagtga cgacaacgag gatcagaatg
    14161 accacagagg tgtccacgac gcctattgtt cacgctcagt ccatgcacgc catccattgg
    14221 ccacgtcttg ttcatctggc gttcatttca ggaccgggct acgggtcaaa aggggcctgt
    14281 ttgggccaag atggacggct atgagcgagg tggactggag cacccccgag gggctggcgg
    14341 cgatcacgac atatctcggc gggcggatcc ccgattggcg ggcgccggtc gcgcatgcgc
    14401 ttgcgctctc gccggccacg agctctccgg agtgggaatt cgcgcacgtc aacgcccccg
    14461 gcgggcgcca cgctctcccg gcggtgatcc ttgcgacggt gctcggccat gacggctcga
    14521 cggcgaccct cgaggtcagc aggtcggacc tgcaggaggc cgtcgcctcg ctcgcgcccg
    14581 ctgcggcctg caccagcctg gaccatccca accttgcggc gtggcggctc gcgctcgcgg
    14641 aggtcgagtc caaccccctg cgcaccatgt gcgccgtctt catcgccgat ctggccgacc
    14701 cggtctcctc ggaggccgac ggctccctgc gcagccgcct ggtctgaagc cgaggcaccg
    14761 gcctcgaacc accccagctg tcgcgaaccc ccggaggact gtcgggcttg ccgaagaaga
    14821 ggcgcccgat gccctgcccg cgggggagta tcccgtcccg cactccaacc gccggcgcgt
    14881 gcttgcctcg tcgggcgcga cctgccaggg tcggcggtat gccgcgacct cacctgaccg
    14941 tctccgctct cgccctcgtc ggcctgctcc cgactgcccc cggactcgcc gcgccggcgc
    15001 ctgcggcaac acctgccacg gcggccgtct tgcgccccgg cgcgccgggg gcgggcgacc
    15061 cgtacttccc cgacatgggc aacggcggct acgacgtcag cagctacgat atcgcgctgg
    15121 cctggaaccc ggcgaccaaa gccatcgccg ccacgaccac gatcaaagcc gttgccacac
    15181 agggactctc ccgcttcaac ctcgacctca agggactcgt ggtgcagagc gtccgggtcg
    15241 gcggggcgag cgcggcatac agcagatcgg gtgcccagga actcgtcatc accccgccga
    15301 agggcatcgc caagggcgcg agtttcaccg tcgccgtgac ctacaagggc gtccccaagg
    15361 cgataaacga ccccaccctc ggccgctccg gctgggtcgc gactgccgac ggcgccgtcg
    15421 cgctcaacca gcccttcggc gccgcgacct acttccccgt caacgacacg ccgcgggaca
    15481 aggcgaccta catccaccgc atcaccgtcc cgaccggcta ccaggtgatc gccaacggcg
    15541 aacccacggc gccgatgacc tcgggcggca acacgacata cgtctggaag atgacctcgc
    15601 cgatggcgag cgagctctcc tccgtcgcca tcggccgcta cacggtgacg acggggaccg
    15661 tcgccggcac gagcatccga tccatctccg ccatcgccaa gagcatcgac aggaccggcg
    15721 ccggcgcccg cctcaacacc gccaccggcg cggtcgtcaa ctggcagcgc aaccggttcg
    15781 gcgcctatcc cttcgcgtcc acgggcgggc tggtggcccc gatcggcgtc ggctacgcgc
    15841 tcgaggtcca gggtcgcccc gtctacgacc agggttcgcc cacggtctcg accggtctgc
    15901 tcgcccacga actcgcccac cagtggttcg gcaacagtgt cacgccgaag cagtggaagg
    15961 acatctggct caacgagggc ttcgcgacgt acgccgaatg gctgtatgac gaggcgcacg
    16021 ggggcccgac cgcgcagcag cagttcactg cccactacaa ctcgaccagt tggaccggca
    16081 aggtcgccga tccggggcgc gaccacatct acgacgggct cgtctacacg cggggcgcgc
    16141 tgacgttgca cgcgctccgc aaccgcatcg gcaccgccac gttcgacaag ctcctcagga
    16201 cctgggcggc cacctatcgc ggacggaatg tcaccacggc cgacttcatc gccacggcgg
    16261 agcgggtcag cggcaagaac ctcgacgcct tcttccagga ttggctctat gagtccggca
    16321 agcccagtct ccggtccgca caatagtttt cgcgatctcg gactgcgggt cggccatgtg
    16381 acgcggaccg gtccagggca gcggaccggc gtcaccgtcg tcctggtcgg ggccgagggt
    16441 gccaccgcag gggtgtcggt gcggggcgcc gccccgggca cgcgggagac cgacctgctc
    16501 tcgcctctgt gcctggtcga ccgggtgcac gcgatcgtcc tctcgggtgg ttcggccttc
    16561 gggctggcga gcgcggacgg ggtggcccgg gcgctgtatg ccgacggcat cggctggccg
    16621 atgggagcac ccggcgaggt ggtcccgatc gtgcccgcgg cggtgatctt cgacctgggg
    16681 cgcggcggcg acttcggcgc ctttccctcc gcggccgacg gtgccgcggc gtatgccgcc
    16741 gcctccctcg ggcaggtcgc gggctgtggc tcggtggggg cggggacggg cgcgcaagcc
    16801 ggtggcctgg cgggcgggct cgggtcggcg agcgccgtgc tgtccgacgg gaccacggtg
    16861 gcggcgctcg tggtcgtcaa cgcgatcggc tcacccgtcg atccccgcac cgggcagctg
    16921 tatgccgtcg cccacggcct cccgggcgag tttcccgatc tgggctcgcc gggagctggc
    16981 gcgctcgcgg catacctgcg ggcgcggcat accgaactcg gcgaatcggg gctcgtcccc
    17041 ggtggcgcga ccaccatcgg ggtcatcgcg accgacgccg cgctcgacaa ggcgcagtgc
    17101 gccaagctcg cggacgtcgg ccacgacggg atcgctcgtg ccatcgaccc ggctcacacc
    17161 atgctcgacg gcgacaccct cttcgcgctc tcgaccgccg cccgtccggc tcctgacctc
    17221 ggcggattgt tcgcgctgca cgaggcggcg gcgtcctgtg tcacgcgagc gatcggtcac
    17281 gcgctgctca ccgcgaccag cgcggcgatg tggccgggga ccggtcagga acgtctcgtc
    17341 gcgtcatacc gcgacgcgtt tgcactctcg gggtgagagt gccaataatg gcgttagcac
    17401 tctccccctg agagtgataa ttcacgacct cgtgggtgag gtcgggtggg acgcggtgac
    17461 gtgagccgcg gatcgcccgg ccgtccgtcg cgggcaccga cgcggtcata ccgaaccaag
    17521 cgtgtggagg caccaccgaa tggccaagat catcgctttc gatgaagagg cacgccgcgg
    17581 tctcgagcgg ggcatgaacg tcctcgccga cgcggtccgg gtcaccttgg gccccaaggg
    17641 acgcaatgtc gtgttggaga agaagtgggg cgcccccacg atcaccaacg acggcgtgtc
    17701 cattgccaag gagatcgaac tcgacgaccc ctacgagaag atcggcgccg agctggtcaa
    17761 ggaggtcgcc aaga
//
LOCUS       DAOPJG010000047        17656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3366_length_17656_cov_7.742231, whole genome shotgun sequence.
ACCESSION   DAOPJG010000047 DAOPJG010000000
VERSION     DAOPJG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17656
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3366_length_17656_cov_7.742231"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="PKU75_06330"
     CDS             <1..131
                     /locus_tag="PKU75_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPF80529.1"
                     /translation="DGEVGNKKQYDPRAWGKEAEAAMAARVVEACQNLRSAGTHVA"
     gene            complement(543..1505)
                     /locus_tag="PKU75_06335"
     CDS             complement(543..1505)
                     /locus_tag="PKU75_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010080265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HPF80530.1"
                     /translation="MAGNSISSGRRDGSRARFEAGVDEKMNADQAAQMTSGKGFIAAL
                     DQSGGSTPKALKLYGIDESAYSNDEEMFDLVHEMRCRIMTSPAFTGERVIAAILFEMT
                     MDRQVEGKDTADYLWEDKHVVPFLKIDKGLAEEADGVQLMKPMPDLDALLARGVAKGI
                     FGTKERSVINAANAAGIQAVVDQQFEVGRQVLSHGLMPIIEPEVNIKAADKQECEDLL
                     KAALLAGLDGVADGQQVMFKLTLPSTDDFYAELIAHPKVMRVVALSGGYSREEANEML
                     ARNPGMIASFSRALSEGLTAQMSDEEFNTTLDASIQAIYDASIT"
     gene            1553..1957
                     /locus_tag="PKU75_06340"
     CDS             1553..1957
                     /locus_tag="PKU75_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HPF80531.1"
                     /translation="MTDLLGIPETHLPDDPAAAMIAEGEERPGRVAAAYPASSLAWAV
                     LAESSLEADHPVQAYAYARTGYHRGLDALRRAGWRGQGPIPWSHEPNRGFLRALAALA
                     KAAGEIDEEDERMRCTEFLRASSPEAARELGL"
     gene            1954..2781
                     /locus_tag="PKU75_06345"
     CDS             1954..2781
                     /locus_tag="PKU75_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80532.1"
                     /translation="MTPEHLLAAFQEQIRLGAGEGSTRSTRERVGHVFRVISSDPDDP
                     WAMVDCPAGLGPDPDAVIAGERDHFASLGIPLEWKTYSYDVPADLGERLTRAGFVKEA
                     DEALMLGELPVLAADVVLPAGVTIREVATHGDARRVKQLYELVWSGAWGRADTDTSRD
                     DELLDAADPNELSLLAEESADGPVLCAARVSLTPGTNFAGMWGGSTHPDWRRKGLFRA
                     LLATRARWALERGATLARVDASPDSEPILTGLGLQRVATTTPYKFGPERDGADTGTS"
     gene            2834..4120
                     /locus_tag="PKU75_06350"
     CDS             2834..4120
                     /locus_tag="PKU75_06350"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPF80533.1"
                     /translation="MPAIVLVGAQWGDEGKGKATDLMGSDVHYVVKFNGGNNAGHTIV
                     IDGEKYALHLLPSGILTPTCTPVIGNGVVIDLEVLFREIDDLEARGVSTEKLVVSANA
                     HVITPYNRTLDKVGERFLGSRKIGTTGRGIGPTYADKMSRNGIRVQDLFDPHILEQKV
                     EAALAFKNQVLTKIYNRRAITVQEVMDELLPYAERLAPMVTDTTLLLEQALNEGRNVL
                     LEAGQATLLDVDHGTYPYVTSSSATAGGACTGSGIPPTRVSRVIAVLKAYTTRVGEGP
                     FPTELFDDDGEFLRKTGAEYGTTTGRPRRCGWMDVVIGRYATRINGVTDFVVTKLDVL
                     TGLEKVPICVAYDVDGVRHDEMPVNQSDFHHAKPIYEEMDGWWEDITGVRTFEDLPAN
                     AQAYILRVEELIGARVSAIGVGPGRDAIIARHPLLD"
     gene            4768..5022
                     /locus_tag="PKU75_06355"
     CDS             4768..5022
                     /locus_tag="PKU75_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80534.1"
                     /translation="MYVKPVVLAPGPGGAPAQPFAWDRTSRTTTALSAAASGEIPDGH
                     TVTPRITTDGRLVAFTSYATNLLPGGSPEGSAYIRTLNAP"
     gene            complement(5095..7332)
                     /locus_tag="PKU75_06360"
     CDS             complement(5095..7332)
                     /locus_tag="PKU75_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF80535.1"
                     /translation="MSTTPSTATTDVVAREIAHEQAHVDRVYAELGRASERAGLVAQD
                     GLARGRTDRTGDIRDEEITGLFERDALMFHAARRQASLDQQYEGLVFGRLDLVDLGTR
                     RVDPVDELPDEWWLDPDREVRYIGRLGVRDEEYEPLVIDWRAPAASAFYQATPVQPQG
                     VIRRRVLRSSGPTVVGVEDDLMAPAAPDDIVITGDGALMAALTRTRGSRMRDIVATIQ
                     RHQDEAIRAPGRGVTEITGGPGTGKTVVALHRAAYLLYSERRRYEGGGVLVVGPSSAY
                     TAYIERVLPSLGEESVLLRSLGDVVDAITTGRLDRPEVAAVKGTLRVRELLKRAARGR
                     VPDAPDTFRAFVGGQAITLAPNVLDGIRRKVLNGHARNAATAAARGMLAEAAWATHRT
                     GERGDFMEAFEESMAVDDFMESWWPQVDPREVLLWLADTDLAYRHGRGVFNDGELAAF
                     AESIRDAIELGSWSVADVALIDDLSARLGLVQEADAEDRGFYEIDELDGRLGAEVRAL
                     GGPVSAADTPAAYRGDTRDALLRGRIGQPDEYAHILVDEAQDLSPMQWRMVGRRAEYA
                     SWTIVGDAAQAAWSDAAEAGRARDDAFGTQPRRTFHLDTNYRNAREIFDYARDVILPL
                     VPDADIPNAVRETGSHPREAVLSLDTLTDVTAEVDRLAEDVAGSIAVISPARYAARLA
                     PLDGAHDNRVVVIDPMSTKGLEWDATVVVDPDAIVDEAPGGIRILYVALTRAAHRMTV
                     LRPVR"
     gene            complement(7397..8422)
                     /locus_tag="PKU75_06365"
     CDS             complement(7397..8422)
                     /locus_tag="PKU75_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF80536.1"
                     /translation="MPEPNGQHPVATAPLAHSWPQMLTEEEISFVYMAIVGHPSPSRE
                     QLLESGIDAHLVDQASRMLHARGLLVIAADGTWEVPSPDIALPAWAADTERRARAWRA
                     TASELHQVYSAARQAKALNSENRDIGTLADIASATATITAGATKEILCIRAASRRTEQ
                     LLLDPYNSHREIFRNAAGHPINVQAIYDPAILDTSGTLEALRERIIGGEEVRLTGPIH
                     FSAIIVDEMAAVIEFSNIDPSGSGALLVRSRPFVTALVRIMRTLWALAVPLSSEAAAP
                     LPSTAASRDAIILKLLAGGATDSTITRRLGISQRTVERRVRHLMDSLGAETRFQAGVE
                     AARQGLV"
     assembly_gap    8833..8932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9278..10558
                     /gene="purD"
                     /locus_tag="PKU75_06370"
     CDS             9278..10558
                     /gene="purD"
                     /locus_tag="PKU75_06370"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPF80537.1"
                     /translation="MKVLVLGSGAREHALVRSLVADPVVDAVVAVPGNPGIAELADCV
                     PVEGGITNGPQVVELATAHHVDLVVIGPEAPLVEGVADDLRAAGIPTFGPSARAAQLE
                     GSKAFAKRIMAEAGVPTSLSHVCTTLDEVGEALDAFGPPYVVKDDGLAAGKGVVVTED
                     RDAALAHAAACFERPDGRIVIEEYLAGPEVSLFCISDGEHVIPLAPAQDFKRVADGDQ
                     GPNTGGMGAYSPLDWIPPHLVEDVVERVAKPTIRQMALLGMPFVGVLYVGLALTARGP
                     KVIEFNVRFGDPETQVVLARLRTPLAGLLHAAATGQLEEVGPLHWHDGAAVTLVLAAE
                     GYPAAPRTGAAITGLETITGETAYVLHAGTATTADGLTAAGGRVLSVVGLGADLAEAR
                     ATAYHALGKIEMPGGHHRSDIALAAQNGQIEVPR"
     gene            10555..11448
                     /locus_tag="PKU75_06375"
     CDS             10555..11448
                     /locus_tag="PKU75_06375"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPF80538.1"
                     /translation="MTQPDAPDLPGYVHVYSGKVRDLYAPTLPDGEPDPDRLLLVASD
                     RLSAFDQILDTPIPDKGRVLTQLSLWWFDQLADLVPNHVISTDVPDAVEGRAVLVRRL
                     DMVPVECVARAYLTGGGLGEYRASGHVSGVALPDGLVDGSRLPEPIFTPSTKAPQGQH
                     DQPMPYADVVAEIGPDRAAEARDLTIAILARGNEIAAGRGILIADTKVEFGIDGDGGI
                     MLADEVLTPDSSRFWPADQWHPGRPQPSYDKQFVRDWLLTESGWDKNSGDAPPSLPDH
                     IVEQTRDRYVEAYARLTGASL"
     gene            11576..12643
                     /locus_tag="PKU75_06380"
     CDS             11576..12643
                     /locus_tag="PKU75_06380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPF80539.1"
                     /translation="MPTTRFVSSRATRTTTIAGLGAGLLVAATALSAHAAGSSYVALG
                     DSYSSGTGTRTYIDDGTSCLRSVYAYPSLIASAKGYALNFRACSGATIADVTNTQLSA
                     LSASTAYVTMSVGGNDAGFARILTTCAQPSWMSDCYGAIDKATAFVNNTLPGSLSTLY
                     TSIRAKAPNAKVTIVGYPRIFNGEDCNAFTWFSPTEEARLNAAADLLNSKLAAAASAK
                     GFAFANPTSRFIGHAVCDSAEWINGLSYPIVNSYHPKVDGHAYGYTPAVSPYVTGSTL
                     RVTSAIKATAAASADRLARQQRAYAAADSRITPKSFVAPDLSTPAAKAAAARAGVDLS
                     SRASIDAADRRYDAAQRAARG"
     gene            12733..13149
                     /locus_tag="PKU75_06385"
     CDS             12733..13149
                     /locus_tag="PKU75_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HPF80540.1"
                     /translation="MPPRRRIAPAAGADALAAWQAAQSSGAVVNREVVATAVRFTLEE
                     LAVRHPGRSVEVRVPPYGAVQCVTGPVHRRGTPPNTVETDADTWLALASGRLTWGAAV
                     DAGRVRWSGIRADLSDYVPVVAEPPGGLPAGYDPDG"
     gene            complement(13131..13283)
                     /locus_tag="PKU75_06390"
     CDS             complement(13131..13283)
                     /locus_tag="PKU75_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80541.1"
                     /translation="MSETSTIDKARNAFAALILATTVVTGGVTVYASETAQPTTASAA
                     HQPSGS"
     gene            complement(13392..14420)
                     /locus_tag="PKU75_06395"
     CDS             complement(13392..14420)
                     /locus_tag="PKU75_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF80542.1"
                     /translation="MTRIESRIPDPMDAPPVRWGILGAGSIARAFAYAVGTGTRSAVT
                     AVGSRDRDKAVAFAAEHDAGHGVGSYEELVDADDVDIVYVATPHSAHHENALLALRAG
                     KPVLVEKAFTRNAVEAAQIVDEARSRRLLCMEAMWSRFLPHYDVVRQLIETGRLGSVR
                     TLTADHGQRLWPEGPQRLSDPGLAGGALLDLGIYPVSFAHMVLGGLQHVRAVGTLTDA
                     GVDATTSIVATGRDGAHALLGTTMGARTPTTATISGDLGRIEIDGDFYMPNTVRLLDS
                     GGAELERWEYDLGTRHFGLRFEAAEAGRCLHAGALESPLLPLDETLSVMRTLDEVRAQ
                     IGVHYPGE"
     gene            complement(14417..15931)
                     /locus_tag="PKU75_06400"
     CDS             complement(14417..15931)
                     /locus_tag="PKU75_06400"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPF80543.1"
                     /translation="MTSTHAGSIRRRRTAAGLAGASAVAMLTATAASAVPSPGAGGIG
                     DPYYPAYGNGGYNVSNYNLFVDYIPGTGRITGTAVITAKATQDLSSFNLDLMLTAKSV
                     TVNGKAAEFAKPDPHELVVKPSLPINNGSTMVVKVKYTGIPSKLSYGGIKPWIGSGTE
                     ALALGEPEIAAWWFPSNDHPRDKASFDMTFTVPKGLEAIGAGDFVGKTSGTTKDTWKW
                     RSPSPMATYLAFMAIGQYDVLTGTANQRPYTTAITASRSDVAVTRARTDLLRTPEVIN
                     WSAAQFGPYPFKQVGGVLAPMDTGFALETQQRPVYTPSFWGGGSNIYVVAHENAHQWF
                     GDSVSVKNWRDIWLNEGFATYAEFLWSDKHGEGTTNQLLEGYYAELGGDAEFWKLPIS
                     NPGAHNEFAWQVYIRGGMALAALRNRVGAEDMTKILKAWTASKKGGNGSIPEFTSLAE
                     RISGEELSAFFDNWLTKRVKPAHTAENGFLSARTGGTAASAARILDNAKLHAAR"
     gene            16023..16265
                     /locus_tag="PKU75_06405"
     CDS             16023..16265
                     /locus_tag="PKU75_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80544.1"
                     /translation="MRERTIMAPGSMTTREPRVVAFIVTGALLGFLLGAGIYLLDEDN
                     GQYSARTAFGYLAVFGLLVGALLGAFAAAIVAGRRR"
     gene            16414..17136
                     /locus_tag="PKU75_06410"
     CDS             16414..17136
                     /locus_tag="PKU75_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPF80545.1"
                     /translation="MAKDESDFRTDEDSGYAAGSGRVVGHERVVTWAEETRRYRRTPG
                     GWWWLALIAIPLLLAALGTAFGDDEPNTRAAGSSTTSVTRSATASTSPAESTSPATSE
                     SSSGASTASESASSSTETSAAADCAGLKDEVGAKLADTNLSFPKSNAELSAGTVSALK
                     DVAGMIARCPDAKVTVSGYTDASGSASENQRISQERADSVRQLLIDNGVAADQVEAVG
                     RAAAEQIPGGIAANRRFEITVK"
     gene            17146..17298
                     /locus_tag="PKU75_06415"
     CDS             17146..17298
                     /locus_tag="PKU75_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80546.1"
                     /translation="MDFVTEWLWYLLAFALGSLVAYVVAAATIKPTNENEAFADVPET
                     RAIGDR"
     gene            17298..>17656
                     /locus_tag="PKU75_06420"
     CDS             17298..>17656
                     /locus_tag="PKU75_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80547.1"
                     /translation="MKWWLFVLAFLLGALLTWIYVVRRATREVRILQDVPVREGEAGL
                     AAGAAAGAAGAATIERPESEAVEVDEVEQPSTGADADTVVDLPPAPAAAADLDTPESA
                     APAGALDLDSEAPALDL"
BASE COUNT         2595 a         6154 c         6017 g         2790 t
ORIGIN      
        1 tcgacggcga ggtcggcaac aagaagcagt acgacccgcg cgcctggggc aaggaggccg
       61 aagccgccat ggccgcccgc gtcgtcgagg cctgccagaa cctgcgctcc gccggcaccc
      121 acgtcgcctg acgttcgcgc cctgtcgtcg aatccggcgt ccggcggcct ctgagccgcc
      181 ggacgccgga tttctcagag cagcagttgc gggctgatgg tgacggggaa cggcctggtg
      241 gccgtccagg tcatctcccc catgacgtcc cgacctgaac gtagtcgccg tccaccagat
      301 cccacgccag caggtggtcc aagggcgcgg tcaggcaccg gagagccggc ggacagctcg
      361 actcgagcct gaccagggtc gcggcgacaa cggcctggtg ttcgcgcgac ggcgatggtg
      421 gccattgtgc cgggcccgcc ggggagcgca aggcgaacgc gagtgccctg cggaaaacct
      481 gccggaccaa gagtccttcc cgcagccgat cacgcggtgc gggaaggact ctcgctgcct
      541 gcttaggtga tggaggcgtc gtagatcgcc tggatgctgg catccagcgt ggtgttgaac
      601 tcctcgtcgc tcatctgcgc ggtcaggccc tcggagagcg cccgcgagaa ggacgcgatc
      661 atgccggggt tgcgggccag catctcgttc gcctcttcgc ggctgtagcc gccggacagc
      721 gcgacgaccc gcatcacctt ggggtgcgcg atgagttcgg catagaagtc gtccgtgctc
      781 ggcagagtga gtttgaacat cacctgctgc ccgtcggcga ctccgtcgag cccggccagc
      841 agcgccgcct tgaggaggtc ctcgcactcc tgcttgtccg cggccttgat gttgacctcc
      901 ggctcgatga tcggcatcag cccgtgcgag agcacctgcc ggccgacctc gaactgctgg
      961 tcgacgacgg cctggatgcc ggcggcgttg gcggcgttga tgaccgagcg ctccttggtc
     1021 ccgaagatgc ccttggccac cccccgcgcc agcagggcgt cgaggtcggg catcggcttc
     1081 atcagctgca ccccgtcggc ctcctcggcc aggcctttgt cgatcttgag gaagggcacg
     1141 acgtgcttgt cctcccacaa gtagtcggcc gtgtccttgc cctcgacctg ccggtccatc
     1201 gtcatctcga aaaggatcgc cgcgatgacc cgctcgcccg tgaaggccgg cgaggtcatg
     1261 atgcggcagc gcatctcgtg gacgaggtcg aacatctctt cgtcgttgct gtaggcgctc
     1321 tcgtcgatgc catagagctt cagggctttc ggggtgctcc caccgctctg gtcgagggca
     1381 gcgatgaacc ccttgccgct ggtcatctgg gctgcctggt cggcattcat cttctcgtca
     1441 actcccgctt cgaatcgtgc ccgcgagccg tcccgccggc cactgctgat gctattgccc
     1501 gccacggccg ttgagaatcc ccagcgagca atcaccccct aatgtgggcg gtatgacaga
     1561 cctgctcggt atccccgaaa cgcaccttcc cgacgacccg gcggcggcga tgatcgccga
     1621 aggtgaggag cgaccggggc gggtcgccgc ggcatacccc gcctcgtcgc tcgcgtgggc
     1681 ggtactcgcc gaatcctccc tggaggccga ccacccggtg caggcctacg cgtatgcccg
     1741 cacgggatac caccgcggac tggacgccct gcgccgggcc ggctggcgcg gccagggccc
     1801 gatcccgtgg tcgcacgagc ccaaccgcgg attcctgcgc gcgctcgccg cgctcgcgaa
     1861 ggcggccgga gagatcgacg aggaggacga acgtatgcgg tgcaccgagt tcctccgcgc
     1921 gtccagtccc gaggcggcac gagagctggg cctatgaccc cggagcacct gctcgccgcc
     1981 ttccaggagc agatccggct cggcgcgggg gagggctcga cgcgctcgac gcgggagcgc
     2041 gtcggacacg tcttccgggt gatctcctcc gatccggacg acccgtgggc gatggtcgac
     2101 tgcccggccg ggctgggccc cgatccggac gccgtgatcg cgggggagcg ggaccatttc
     2161 gcttctctcg ggatcccgtt ggagtggaag acctattcgt atgacgtgcc cgccgacctc
     2221 ggcgaacgtc tgacccgcgc gggcttcgtc aaggaggcgg acgaggcgct gatgctcggg
     2281 gagttgccgg tgctcgccgc agacgtcgtc ctgccggccg gcgtgacgat ccgggaggtc
     2341 gccacccacg gcgacgcccg ccgagtcaag cagctgtatg aactggtctg gtcgggagcg
     2401 tgggggcgcg ccgacaccga cacgagccgc gacgacgagc tgcttgacgc ggctgacccg
     2461 aatgagctct ccctgctggc cgaggagtcc gctgacggtc cagtcctctg cgcggcccgc
     2521 gtaagcctca ctcccggaac caatttcgcc ggcatgtggg gcggctcgac ccacccggac
     2581 tggcgccgca agggcctctt ccgcgccctg ctcgccaccc gcgcccgctg ggcgctggag
     2641 cggggcgcga ccctcgcccg cgtcgacgcc tctcccgact ccgagcccat cctgactggc
     2701 ctcggtctgc aacgggtcgc gacgaccacg ccctacaagt tcggcccgga gcgagacggc
     2761 gcggacacgg ggacgagctg acgcggcata cgactagcct ggctgttggt tcaccgacga
     2821 aggggagtag gacatgccgg cgatcgtgct ggtcggcgcc cagtggggcg acgagggcaa
     2881 gggcaaggcc accgacctca tggggtcgga cgtccactat gtcgtgaagt tcaacggcgg
     2941 caacaacgcc gggcatacga tcgtcatcga cggcgagaag tacgcgctgc acctgctgcc
     3001 cagcggcatt ctcaccccca cgtgcacgcc ggtcatcggc aacggcgtcg tcatcgacct
     3061 cgaggtgctc ttccgggaga tcgacgacct ggaggcgcgg ggggtgtcca ccgagaagct
     3121 cgtcgtcagc gccaacgcgc acgtcatcac gccgtacaac cgcaccctcg acaaggtcgg
     3181 ggagcgcttc ctcggctcgc gcaagatcgg cacgacgggc cgcggcatcg ggccgaccta
     3241 tgcggacaag atgtcccgca acgggattcg cgtccaggac ctcttcgacc cgcacatcct
     3301 cgagcagaag gtcgaggcgg cgctggcgtt caagaaccag gtgctgacca agatctacaa
     3361 ccggcgcgcg atcaccgtcc aggaggtcat ggacgagctg ctgccgtatg ccgagcggct
     3421 ggccccgatg gtcaccgaca cgacactcct gctcgagcag gctctcaacg agggacgcaa
     3481 cgtcctgctg gaggccggcc aggcgaccct cctcgacgtc gaccacggca cctacccgta
     3541 tgtgacctcc tcctcggcca cggcgggcgg ggcgtgcacg ggctccggca tcccgccgac
     3601 gcgggtgtcg cgggtcatcg cggtcctcaa ggcctacacc acccgggtcg gcgaagggcc
     3661 tttccccaca gagcttttcg acgacgacgg cgagttcctg cgcaagaccg gcgcggaata
     3721 cggcaccacg accggccgcc ctcgccgctg cggctggatg gatgtcgtca tcggccgcta
     3781 cgccacccgc atcaacggcg tgaccgactt cgtggtcacc aagctcgacg tcctcaccgg
     3841 cctcgagaag gtcccgatct gcgtcgccta cgacgtcgac ggcgtccgcc acgacgagat
     3901 gccggtcaac cagagcgact tccaccacgc caagcccatc tacgaggaga tggacggctg
     3961 gtgggaggac atcaccggcg tgcggacctt cgaggacctg ccggcgaatg cccaggctta
     4021 catcctgcgc gtcgaggaac tcatcggcgc ccgggtctcc gcgatcggtg tcggccccgg
     4081 ccgcgacgcg atcatcgcac gccacccgct gctggattga gcggtatgcc gttgccggcg
     4141 acaacatcct tcagtgggtg ttgccgttcg tgacggcacg cgacggggtg ctggtatgga
     4201 gtgcaccctg cctcgcatcc gacgtcgagc gagcctgacc ctcgcggccc tggttgccgt
     4261 ctggtccttg accgcgctcg ccgtggccgg ccctgcgcgg ctcctcgcgt cgggggcggg
     4321 ggtgatcgac atgagcgaga ccggacgctt cgtcctgctt caccgagagg ggcgtgttcc
     4381 gcactatgtg cgcttggacc gcgccaccgg cgaaaccctc ggcctgcgtg acatccgtgg
     4441 gcagacccgg atggcgccgg gtggccgggt gttcttctgg aatcccgacg gacagttcga
     4501 catctatcgt caggtcggca cgaaccgtcc gagcctggtc ttcgatgccc ccaacccgca
     4561 gggctggtat ttcgactacg acggcgccag cgccgacggc tccctggtgg tcggacaagc
     4621 actccgtgaa gagccacgtg cggccgtcgc ggccgtcctg gacaccaaca ccggtgggct
     4681 gagattcctc gcgccgctgg agggcacgtc cggcgacgtc cggtgccctt ctcggatctc
     4741 aacatcgggg gcgatggttc gcgcatcgtg tacgtcaagc cggtggtcct cgcccctggg
     4801 cccggcggcg ctccggcgca gccgttcgcg tgggatcgca catcgcggac gacgaccgcc
     4861 ctgtccgctg ctgccagcgg ggagattcct gacggtcaca ccgtcactcc ccgcatcacc
     4921 accgatggcc ggctggtggc gttcacgagc tacgcgacca acctcctccc gggaggaagc
     4981 cccgaagggt cggcgtacat ccgcaccctg aacgcaccct gaacgcgccg tcacccggcg
     5041 agcctgaccg ggcgccgatc gctagggggc gccgctgtgc acgtgtggtc ttgatcagcg
     5101 caccgggcgc agcaccgtca tccgatgcgc tgcccgcgtc aaggcgacat agaggatccg
     5161 gatgccgccg ggcgcctcgt cgacgatggc gtcagggtcg acgaccacgg tggcgtccca
     5221 ctccagtccc ttcgtcgaca tcgggtcgat gacgacgacg cggttgtcgt gggcgccgtc
     5281 caggggggcc aggcgcgcgg cataccgggc tgggctgatg acggcgatgg agccggccac
     5341 gtcctcggcc agccggtcca cctccgcggt gacatcggtc agcgtgtcga ggctcagcac
     5401 ggcctcccga ggatgactgc cggtctcgcg caccgcattc gggatatcgg cgtcgggcac
     5461 gaggggcagg atcacgtctc tggcgtagtc gaagatctcg cgggcattgc ggtagttcgt
     5521 gtcgaggtgg aaggtgcggc gcggctgggt gccgaacgcg tcgtcgcggg ctcggccggc
     5581 ttccgcggca tcggaccacg cggcttgcgc cgcgtcgccg acgatcgtcc acgacgcata
     5641 ctccgcgcgc cgcccgacca tccgccactg catgggcgag aggtcctggg cctcgtcgac
     5701 gagaatgtgc gcgtattcgt cgggctggcc gatccgcccg cgcagcaacg catctcgcgt
     5761 atcaccgcgg tatgccgcgg gcgtgtccgc agcgctcacg ggcccgccca aggcgcgcac
     5821 ttcggcgccc agccggccgt cgagttcgtc gatctcgtag aagccgcgat cctcggcgtc
     5881 cgcctcctgg accaggccga gccgcgccga gaggtcatcg atcagggcaa cgtctgcaac
     5941 cgaccaggag ccgagttcga ttgcgtcgcg gatcgattcg gcgaaggcgg ccagttcgcc
     6001 gtcgttgaag acgccgcggc cgtgccggta ggcgaggtcc gtgtcagcca gccacagcag
     6061 cacctcgcgc gggtcgacct gcggccacca ggactccatg aagtcgtcga ccgccatcga
     6121 ctcctcgaag gcctccatga agtccccgcg ctcacccgtg cggtgagtgg cccacgctgc
     6181 ctccgccagc atcccgcggg cagcggcggt cgcggcattg cgggcatgac cgttgagtac
     6241 cttccggcgg atgccgtcaa gcacgttggg cgccaacgtg atcgcctgcc cacccacgaa
     6301 cgcgcggaag gtgtccggcg cgtccgggac gcgaccgcgg gccgcccgct tcagcagttc
     6361 acggacccgc agcgttccct tgacggccgc gacctcgggc cggtccaggc gtccggtcgt
     6421 gatggcgtcg acgacatcgc cgagcgagcg cagcaggacg gattcctcac cgagagaagg
     6481 caagacgcgc tcgatgtatg ccgtgtaggc gctcgagggc ccgacgacga ggactccacc
     6541 cccctcatac cgtcggcgtt cgctgtagag caggtatgcc gcgcggtgca gcgccaccac
     6601 cgtcttgccc gtgccggggc cgccggtgat ctccgtgacg ccacggcccg gggcgcggat
     6661 cgcttcgtcc tggtggcgct ggatggttgc gacgatgtcg cgcatccgac tgccccgggt
     6721 gcgggtgagc gcggccatca gggcgccgtc accggtgatg acgatgtcgt cgggtgcggc
     6781 cggtgccatc agatcgtcct cgacgccgac gacggtcggg ccggagctgc gcaatacgcg
     6841 ccgccggatc accccttgcg gctggaccgg cgtggcctga taaaacgccg acgcagccgg
     6901 tgcgcgccag tcgatgacga ggggctcgta ctcctcgtcg cggaccccga gccgcccgat
     6961 atagcgcact tcgcgatccg ggtcgagcca ccactcgtcg ggcaactcgt ccacggggtc
     7021 aactcgtcgc gtccccaggt ccaccaggtc gagccggccg aagaccaggc cctcgtactg
     7081 ctgatccagg ctggcctgac gccgcgcggc atggaacatc aaggcgtcgc gttcgaagag
     7141 cccggtgatc tcctcgtcac ggatgtcacc ggtgcgatcg gtccggccgc gggccagacc
     7201 gtcctgggcg accaggccgg cgcgctcgct ggcgcggccc agttcggcat acacccgatc
     7261 gacgtgggcc tgctcgtggg cgatctcccg ggcgaccaca tcggtagtcg cggtggaggg
     7321 ggtggtgctc actgtgtctc gcgttcggtc agctgactcg ggaacacatc agtctatgcc
     7381 cacccggcgg cggcaatcag accagcccct ggcgggccgc ctcgactccc gcctggaagc
     7441 gcgtttcggc cccgagactg tccatcaggt gccgcacccg gcgctccacc gtgcgttgag
     7501 agatgcccag ccgccgggtg atcgtggagt ccgtcgcccc gccggcgagg agcttgagga
     7561 tgatggcatc gcggctggcg gccgtggacg gcagcggcgc cgcggcctcg ctcgagagcg
     7621 gcacggcaag cgcccacagg gtgcgcatga tgcgcacgag cgcggtgacg aagggccggc
     7681 tgcgcaccag gagcgcgccg ctgccggagg ggtcgatatt gctgaactcg atcaccgcgg
     7741 ccatttcgtc gacgatgatg gccgagaagt ggatggggcc ggtcagccgt acctcctcgc
     7801 cgccgatgat tctctctcgc aacgcttcca gcgttccgga cgtgtcgagg atggccgggt
     7861 cgtagatcgc ctgcacattg atggggtgcc cggccgcgtt gcggaagatc tcgcggtgcg
     7921 agttgtaggg gtcgaggagc aactgctcgg tgcgacgcga cgcggcccgg atgcagagga
     7981 tctccttggt cgcgcccgcg gtgatggtgg ccgtcgcgga ggcgatgtcc gcgagcgtgc
     8041 cgatgtcccg attttccgag ttcagtgcct tggcctgccg ggccgccgaa tacacctggt
     8101 gcagttcgct cgccgtcgct cgccacgcgc gggcccgccg ctccgtgtca gcggcccagg
     8161 ccggcagggc gatgtcgggg gacggcacct cccaggtgcc gtcggccgcg atgaccagca
     8221 gcccgcgagc gtgcagcata cggctggcct ggtcgacgag gtgggcgtcg atgccgctct
     8281 ccagcagctg ttcacgactc ggcgaggggt ggccgacgat cgccatatag acgaagctga
     8341 tctcctcctc ggtgagcatc tgcggccaac tgtgcgccag cggcgcggtg gcgacggggt
     8401 gctgaccgtt gggttcgggc atctggcaag cgtgccaggc tggcgggttt ccgtcaatgt
     8461 cggggatgcg acaggtttcg actttactcg cgggtagaac attgtttaat ctttcattgg
     8521 ttgatcccgc gtcaacccgc acgcagagcg ccggttcccc caactgttgc gccgcgtgcc
     8581 tcggcgcggc gttcccgggg gaatcccccc tgctccccgg gaacgccgcg ccgaacctat
     8641 ttcttcttgt tcttctcctc cgctggttct tcttgttctt ctcctccgct ggctcggcgg
     8701 gtccgctcgt tcctcgcggc cccgtctcgc cgctgcgtcg aagaacgcgg cgtcgagtgc
     8761 tcatttggct ggcttgcgct gccgtctcgc catcgggctg tcgggtgccc gctcgttcca
     8821 accgtttctt ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttcttct
     8941 cctgcgccgg ctcggcgggt ccgctcgttc ctcgcggtcc cgtctcgccg ctgcgtcgaa
     9001 gaacgcggag tcgagtgctc atttggctgg cttgcgctgc cgtctcgcca tcgggctgtc
     9061 ctcgcggtct ccccagaccc gccggcgatc gcctaaccat tcggtaaccg aatcgaagcg
     9121 acaggtgcgg gcgccatcgg cagacgccac gagcggcgga caccacgagc gactgacggc
     9181 ccgagcgaca gacgccacgg ggcggtccgg ctccgagcca ccgaccgacg gcgagatgag
     9241 aacgaacgag atcggaacga acactaggct cggcggcatg aaggtgttgg tgctggggtc
     9301 cggcgctcgc gaacatgctc tcgtccggtc cctggtggcc gacccggtgg tggatgcggt
     9361 ggtcgcggtg ccgggcaacc ccgggatcgc cgaactcgcg gactgtgtgc ccgtcgaggg
     9421 gggcatcacc aatggcccgc aggtcgtcga gctggccaca gcacatcacg tcgatcttgt
     9481 ggtcatcgga ccggaggcgc cgttggtcga gggggtcgcg gacgacctgc gggcggccgg
     9541 catacccacc ttcgggccct ccgcccgcgc tgcccagctc gagggcagca aggccttcgc
     9601 caaacggatc atggccgagg cgggagtgcc gacctccctc tcccacgtct gcaccaccct
     9661 cgacgaggtc ggcgaggctc tcgacgcgtt cgggccgccc tatgtggtca aggacgacgg
     9721 cctggccgcc ggcaagggag tcgtcgtcac cgaggaccgc gacgccgccc tcgcccacgc
     9781 cgccgcctgc ttcgagcggc ccgacggacg catcgtcatc gaggagtacc tcgccgggcc
     9841 cgaggtctcc ctgttctgca tcagcgacgg cgaacacgtc atacccctcg cccccgcgca
     9901 ggacttcaaa cgcgtcgccg acggcgacca aggcccgaat accggtggta tgggcgcata
     9961 ttcgcccctc gactggatcc cgccacatct tgtggaggac gtcgtggagc gggtcgccaa
    10021 gccgacgatc cgccagatgg ccctgctcgg catgcccttc gtcggggtgc tgtatgtcgg
    10081 tctggccctg accgcgcgcg ggcccaaggt catcgagttc aacgtccgat tcggcgaccc
    10141 cgagacacag gtcgtgctcg cccggctgcg cacccctctg gcgggactgc tgcacgccgc
    10201 cgcgaccggc cagttggagg aggtcggccc cctgcactgg cacgacggcg ccgcagtcac
    10261 cctcgtcctc gcggccgagg gctacccggc agcgccgcgg acgggcgccg cgatcaccgg
    10321 cctcgagacg atcacgggag agacggcata cgtcctgcac gccggcaccg cgaccaccgc
    10381 cgacgggctg accgcagccg gcggccgggt gctgtcggtc gtcgggctcg gcgccgacct
    10441 cgccgaggcg cgggccacgg cataccacgc cctcggcaag atcgagatgc ccggcgggca
    10501 ccaccgcagc gatatcgcgc tcgcggccca aaacggacag atcgaggtac cccgatgacc
    10561 cagcccgacg cgcccgacct gcccggctac gtccatgtct actcgggcaa ggtccgcgac
    10621 ctctacgcac cgacgttgcc cgacggcgaa cccgacccgg accggctgct gctcgtcgct
    10681 tccgaccggc tctcggcctt cgaccagatc ctcgacaccc ccattcccga caagggccgg
    10741 gtgctgacgc agctgtcgct gtggtggttc gaccaactcg ccgacctggt gcccaaccac
    10801 gtcatctcca ccgacgtgcc cgacgcggtc gagggccggg cggtcctcgt gcgccgcctc
    10861 gacatggtgc ccgtcgagtg tgtcgcccgg gcgtatctga ccgggggcgg gctgggggag
    10921 tatcgcgcgt cgggtcacgt ctccggggtc gcgctccccg acggcctcgt cgacggctcg
    10981 cgcctgccgg agccgatctt caccccatcg accaaggcgc cccagggcca gcacgaccag
    11041 ccgatgccgt atgccgatgt cgtcgccgag atcggtcccg accgagcggc cgaggcccgt
    11101 gacctgacga tcgcgatcct ggcccggggc aacgagatcg ccgccggacg cggcatcctc
    11161 attgccgaca ccaaggtgga gttcgggatc gacggggacg gcgggatcat gctcgccgac
    11221 gaggttctca cccccgattc ctcccgcttc tggccggccg accagtggca cccgggccgg
    11281 ccccagccgt cctacgacaa acagttcgtc cgcgactggc tgctgaccga gagcggctgg
    11341 gacaagaatt ccggcgacgc gccgccttcg ctgccggatc acatcgtcga gcagacccgc
    11401 gaccgctacg tcgaggccta cgcacggctg accggcgcct ccctataacg acgcgtcgat
    11461 tccgcacccg gaggtcttcc cgggccggtg cggcaacctc tactctgcgg taaccacaca
    11521 ccgtcgtgtg gtttccatca cgctgatccg gcgtggcaga ccgagggagc cccccatgcc
    11581 caccacccgc ttcgtctcct cgcgcgcgac gcgcaccacc accatcgccg gcctcggcgc
    11641 cggcctgctc gtcgcggcga cggccctcag cgcgcacgcc gccggctcgt cctatgtcgc
    11701 tctcggcgac agttactcct ccggcaccgg cacccggacc tatatcgacg acggcacctc
    11761 gtgcctgcgg tccgtctacg catatccgag cctgatcgcc agcgccaagg gctacgccct
    11821 caacttccgc gcctgctccg gcgcgaccat cgccgacgtg acgaacaccc agctttcggc
    11881 cctcagcgcg tccacggcat acgtcacgat gtcggtcggt ggcaacgatg ccggcttcgc
    11941 cagaatcttg acgacctgcg cgcagccgtc gtggatgagc gactgctacg gcgcgatcga
    12001 caaggcgacg gccttcgtca acaacactct ccccgggtcc ctgtcgaccc tctacacctc
    12061 gatccgggcc aaggccccca acgccaaggt gaccatcgtc ggctacccgc gcatcttcaa
    12121 cggcgaggac tgcaacgcct tcacctggtt ctcgccgacc gaggaggccc gcctcaacgc
    12181 cgccgctgac ctgctcaaca gcaagctggc cgccgcggca tccgccaagg gtttcgcctt
    12241 cgccaacccg acgtcgcggt tcatcggcca cgccgtctgc gacagcgcag agtggatcaa
    12301 cggcctgtcc tacccgatcg tgaactccta ccacccgaag gtcgacggcc atgcctacgg
    12361 ctacacgccg gccgtcagcc cgtacgtgac cggctcgacg ttgcgcgtca cctcggccat
    12421 caaggcgacc gccgccgcgt cggccgaccg gctcgcccgc cagcagcgcg cgtatgccgc
    12481 tgcggactcc cgcatcacgc ccaagagctt cgtcgcgccc gacctgagca cgcccgcggc
    12541 gaaggccgcc gcagcgcggg cgggtgtcga cctgtcttcg cgcgcgagca tcgacgccgc
    12601 cgaccggcgg tatgatgccg cgcaacgcgc cgcgcgtggc tgaccgttag ccgccacccc
    12661 gcgacggcag gggcaaccgg ccccgaggct tttcgtgcct gggggccggt tcgcgctggc
    12721 aggattgccg gtatgccgcc gcgtcgccga atcgcccccg cagccggtgc cgacgcgctg
    12781 gccgcgtggc aggctgcgca gtcctccggg gcggtggtga accgcgaagt tgtggccacg
    12841 gcggtgcggt tcacgttgga ggaactggcc gtgcgtcatc ccggacgcag tgtggaggtg
    12901 cgggtgccgc cctacggggc ggtgcagtgc gtcaccggac cggtgcaccg gcgcggcacc
    12961 ccacccaaca cggtcgagac cgacgccgac acctggctgg cgctcgcctc cggccggctg
    13021 acctggggcg cggccgtcga cgccggccgt gtgcggtgga gcggcatccg ggccgacctt
    13081 tcggactatg tgcccgtggt ggccgaaccc cccggcggac tgccggccgg ttatgacccc
    13141 gacggctgat gagccgcgga cgcggtggtg ggctgggccg tctcggacgc gtagaccgtg
    13201 actccgccgg tcacgaccgt tgtcgcaagg atgagggcgg cgaaggcatt gcgggccttg
    13261 tcgatggtgg acgtctcgga catggtggcg ctcccttcac gggccgggca ggtggtcggt
    13321 cctgataccg atgagcctga ggctccctgc tgtggcgtcg ctgtgcgggg cctgccaatc
    13381 cggtgtggcc gtcattcgcc cgggtagtgc acgccgatct gggctcgcac ctcatcgagg
    13441 gtgcgcatga cggagagggt ctcgtcgagg ggcaggagcg gcgactcgag ggcgcccgca
    13501 tgcaggcagc ggccggcttc ggcggcttcg aaacgcagcc cgaagtgccg agtgccgagg
    13561 tcgtattccc agcgttcgag ttcggcgccg ccggagtcga gcagtcggac ggtgttgggc
    13621 atgtagaagt ccccgtcgat ctcgatgcgt ccgaggtcac ccgagatggt ggccgtggtg
    13681 ggcgtgcgcg cccccatcgt cgtgcccagc agcgcgtggg caccgtcgcg gcccgttgcc
    13741 acgatgctcg tggtggcgtc gacgccggca tcggtgagcg tgccgacggc ccgcacgtgt
    13801 tggaggccac ccaagaccat gtgggcgaac gagaccgggt agatgcccag gtcgagcagg
    13861 gcgccgccgg cgagcccagg gtcggacagg cgttgcggtc cctcgggcca gaggcgctgg
    13921 ccatgatcgg cggtcaaggt gcggaccgag cccagccgcc ccgtctcgat cagctggcgg
    13981 accacgtcat agtgcggcaa aaaccgcgac cacatcgcct ccatgcacag cagccgcctc
    14041 gaccgggctt cgtcgacgat ctgagctgct tcgacggcgt tgcgcgtgaa cgccttttcg
    14101 accagcaccg gtttgccggc gcgcagcgcg aggagggcgt tctcgtggtg ggccgagtgc
    14161 ggggtcgcga cgtagacgat atcgacgtcg tcggcgtcca cgagttcctc gtagctcccg
    14221 actccgtgtc ccgcgtcgtg ctcggccgcg aaagcgaccg ccttgtcccg atcacgagag
    14281 ccgacggccg tcaccgcgga ccgggtgccg gtgcccacgg catacgcgaa agcgcgtgcg
    14341 atcgagccgg ccccgaggat gccccaccgc acgggcgggg catccatcgg gtccgggatg
    14401 cggctctcga tgcgggtcaa cgggcggcgt gcagcttcgc gttgtcgagg atgcgggccg
    14461 cggacgctgc cgtgccgccc gtgcgggcag acaagaaacc gttctcggcc gtgtgggccg
    14521 gcttgacgcg cttggtcagc cagttgtcga agaaggcgct cagctcctca ccggagatcc
    14581 gctcggcgag ggaggtgaac tcgggaatgc tgccgttgcc gcccttcttg ctggcggtcc
    14641 acgccttgag gatcttcgtc atgtcctccg cgccgacgcg gttgcgcagg gccgccagcg
    14701 ccatgccgcc gcggatatag acctgccacg cgaactcgtt gtgggcgccc gggttggaga
    14761 tcgggagctt ccagaactcg gcgtcacctc cgagctcggc gtagtagccc tcgagcagct
    14821 ggttggtcgt gccctcgcca tgcttgtcgg accacaggaa ctcggcgtag gtcgcgaagc
    14881 cctcgttgag ccagatgtcg cgccagttct tgacactcac cgagtcgccg aaccactggt
    14941 gggcgttctc gtgcgccacg acatagatat tgctgccgcc gccccagaag gacggggtgt
    15001 agaccggccg ctgctgggtc tcgagcgcga agccggtgtc catcggggcg agaacgccgc
    15061 cgacctgctt gaacgggtag gggccgaact gcgcggccga ccagttgatg acctcggggg
    15121 tgcgcaacag atccgtccgg gcccgggtga cagccacatc ggaccggctc gcggtgatgg
    15181 ccgtggtgta gggccgctgg tttgcggtgc cggtcagcac gtcatactgc ccgatcgcca
    15241 tgaaggccag ataggtcgcc atcggtgacg gggagcgcca cttccacgtg tccttggtcg
    15301 tcccgctggt cttgccgacg aagtctccgg caccgatggc ctccagcccc ttgggcacgg
    15361 tgaaggtcat gtcgaagctc gccttgtcgc gcgggtggtc gttgctcggg aaccaccagg
    15421 cggcgatctc cggctcaccc agcgccagcg cctcggtgcc cgaaccgatc cagggcttga
    15481 tgccgccata cgacagcttg ctcgggatgc cggtgtactt gaccttgacc accatcgtcg
    15541 acccgttgtt gatcggcagc gagggcttga cgaccagctc gtgcgggtcg ggcttggcga
    15601 actcggccgc cttgccgttg accgtcaccg acttggccgt gagcatcagg tcgaggttga
    15661 aggacgagag atcctgggtt gccttggccg tgatcacggc cgtgccggtg atgcggccgg
    15721 tgcccggaat gtagtcgacg aagaggttgt agttggagac gttgtagccg ccgttgccgt
    15781 aggccgggta gtaggggtcg ccgatgcccc cagcaccggg ggaggggacg gccgaggcgg
    15841 cggttgccgt cagcatggcg acggccgaag ccccggcgag gccggccgcc gtgcggcggc
    15901 gacgaatcga tccagcatgc gtgctcgtca tgggtggtcc tccctgtcgt ggaacgaaca
    15961 ggtgacacac tagggggcga ggccgacaac cgcccgtggc tggcggggat gtcgggtaac
    16021 gcgtgcgtga aaggacgatc atggcccccg gctcgatgac gacccgcgaa ccccgggtgg
    16081 tggccttcat cgtgaccggc gcgctgctgg gcttcctcct cggcgccggg atctatctcc
    16141 tcgatgagga caacggtcag tactccgcac gcaccgcttt cggctatctc gccgtcttcg
    16201 ggttgctcgt cggggcgctc ctgggtgctt tcgccgcggc catcgtcgcc ggccgtcgtc
    16261 gttagtgggc tcagccggca gcgtccggac gcaacgtgac gaagatggcg cgaacctctc
    16321 cgtcgtgcca cgtatcggct gtcggctcgg acccgtaatc tgagttggcg cgggcccccc
    16381 gagggggcac gccgagtcga aacggagcag ctgatggcga aggacgagtc cgatttccgg
    16441 accgatgagg attccgggta tgccgcgggg tcgggccgcg tggtcggcca cgagcgggtg
    16501 gtgacctggg ccgaagagac ccggcgttat cgccgcaccc ccggcggctg gtggtggctg
    16561 gccctgatcg ccataccgtt gctgctcgct gcgctcggga cggccttcgg cgacgacgag
    16621 cccaacacca gggcggcggg ttcgtcgacg acctcggtga cccgctcggc aacggcttcg
    16681 acctcacccg ccgagtccac gtcgccggcc acgtccgaga gcagcagtgg cgccagcact
    16741 gcaagcgagt cggccagctc gagcacggag acgtcggcgg ccgccgattg cgctggcctc
    16801 aaggatgagg tgggcgccaa gctcgccgac accaacttgt cgttccccaa gtcgaacgcg
    16861 gagttgagcg ccggcacggt ctcggcgctc aaggacgtcg ccgggatgat cgcccggtgt
    16921 cctgacgcca aggtcacggt gagtggctac acagacgcct cgggctcggc cagcgagaac
    16981 cagcgcatca gtcaagaacg ggccgattcc gtacgccagc tgttgatcga caacggcgtt
    17041 gcagcggacc aggtcgaggc cgtcggcagg gccgccgcgg agcagatccc tggcggcatc
    17101 gccgcgaacc ggcgctttga gatcacggtg aaataggagg cgacgatgga tttcgttacg
    17161 gaatggctct ggtatctact ggcgttcgcg ctgggctcgc tcgtcgccta tgtggtcgcc
    17221 gcggccacca tcaagcccac caatgagaac gaggcgttcg ccgacgttcc cgaaactcgt
    17281 gcgatcggag accgctgatg aagtggtggt tgttcgttct ggcgttcctg ctgggcgcct
    17341 tattgacgtg gatctatgtg gtccgccgcg cgactcgcga ggtgcgcatc ctccaggatg
    17401 tgccggtccg cgagggcgag gccggcctcg ccgccggtgc ggcggccggt gctgccggtg
    17461 cggcaacgat tgagcgaccc gaatccgagg cagttgaggt cgacgaggtc gagcagccgt
    17521 cgaccggcgc cgacgcagac accgtggtcg atctgccccc cgcgccagcg gccgccgccg
    17581 atctcgacac gccggagtcc gcagcccccg ccggtgcgct cgacctcgac tccgaggctc
    17641 ccgcccttga cctgga
//
LOCUS       DAOPJG010000048        17630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3372_length_17630_cov_7.743044, whole genome shotgun sequence.
ACCESSION   DAOPJG010000048 DAOPJG010000000
VERSION     DAOPJG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17630
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3372_length_17630_cov_7.743044"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2394
                     /locus_tag="PKU75_06425"
     CDS             <1..2394
                     /locus_tag="PKU75_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPF80548.1"
                     /translation="QAFDAAGNSSPAVRITFRTKVSAESQPPSTPTNLTADPTSDAVT
                     LTWTPSTDNVKVAQYVVTVGSLTLAVTEPRTTITGLTADTAYRATVMAKDTSGNTSGE
                     AAVDFRTVTTADTQAPTAPTALSATPTTNAVTLTWTASTDNVGVVGYDVTIGSTTQTV
                     TGSPATVTGLSPTTAYRATVVAKDAAGHVSTPAVIDFRTATPADTQAPTAPTGLSATP
                     TTDAVTVNWTASTDNVGVAAYDVTVGDTTKTVTGTSAAFTGLTPTTAYRATVVAKDAA
                     GNSSTPATVDFRTTTPPDTQAPTAPTNLRATADSTSASLTWTASSDNVGVTGYVVSVG
                     SITQTVTTTSATVTRLSAATTYTARVVAEDAAGNQSTGASVTFTTATNPTGKVIYVSP
                     DGSDANPGTAGSPKRTIQAAVDLAEPGTVIRLYAGTYANGTTIRIRTSGRSDAPIVIQ
                     PAGNGEVTVTHPVTTASCSNSAPASDRTFTFSNGVDHWRIENLNIHNGVWIAGKRSNY
                     AYNWLTNYVNNGDWASRRRAPGHGEYNPAAARTDLVPFLRAQTGQSDLDMAEGITIRG
                     NHLTGRGVYGALTSYGEISGNRIDQIPCGIGPGIWLMTFSNGWKIFDNDVSDIAISHA
                     AHYMQEGIRVGSAANYNEIYDNYVHDLPGDGRGINTDVDGSWNHIHHNRVENVAIGYN
                     DQMSGWGNRWEYNTVTNYRVYGMGFRLKDATLVSPSLASSTNLVKVRCNVTANPVGDR
                     AKALGVGASIDGTWIGNTMPKIWLSKNLRVYWTAQGNTWNGSSALPGDYPPQDTSMC"
     gene            2444..3343
                     /locus_tag="PKU75_06430"
     CDS             2444..3343
                     /locus_tag="PKU75_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPF80549.1"
                     /translation="MTTPPLPPGRLRVGVVGAGKVGAVLGAALANAGHLVVSACAVSE
                     ASRARAAALLPDAPIRTVDEVVDESDLVLLAVPDDTLSGLVQGLATTGVWRLGQLALH
                     TSGRSGLAPLAPAIAQGVGGMAIHPAMSFTGTAMDVERLRDCCFGVTAPPELIAPACA
                     LVVEMGGEPLVIDEFDRVRYHLGLAHSANYLVTLVAQAAQVLQGAGVEDTDRLLRPLL
                     SAALDNALRQGDSALTGPVARGDAGTVAEHLEVLERETDDVREVYRVLAEATVARALR
                     GRRLSEAGARSIREALDRPKETP"
     gene            3340..4281
                     /gene="panC"
                     /locus_tag="PKU75_06435"
     CDS             3340..4281
                     /gene="panC"
                     /locus_tag="PKU75_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPF80550.1"
                     /translation="MTTTTERRAPAVARTRDELREARKALTGKEIAVVMTMGALHDGH
                     ATLVKQARAEADHVIVTIFLNPLQFGPREDLSRYPRTFDSDMEICTEMGADLVFAPTP
                     DVIYQDGDPGVRVSAGPLGDVLEGQSRPGHFDGMLTVVAKLMHLTGGTAFFFGQKDAQ
                     QLLLIRRMVRDLDFPGRVISVPTVREDDGLAMSSRNTYLTESDREVALSLSKALKAGA
                     AAAPEGPSAIRRAARAVLVAEPLVLVDYLVLVHPTTLKDVPEWYRGEALLAVAGKVGT
                     TRLIDNMPVLVGPGGGALSVFSNPDDLGPAEAAGGAR"
     gene            4286..4711
                     /locus_tag="PKU75_06440"
     CDS             4286..4711
                     /locus_tag="PKU75_06440"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPF80551.1"
                     /translation="MLRNMLHCKIHRATVTQADLHYVGSLTIDRDLMDAAHMLPGQQV
                     DVVDVDNGHRLTTYAIEGERGSGIVCINGAAARLISPGDLVIIIAYALMDEEEARTFE
                     PQVVFVDKRNRIVEVGHDGGDVPDGYGLKTSAVTKRRHA"
     gene            4704..6392
                     /locus_tag="PKU75_06445"
     CDS             4704..6392
                     /locus_tag="PKU75_06445"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPF80552.1"
                     /translation="MPELATLPPVRIPRYLLAGAPGWTTRADVIVVGSGIAGLTCALR
                     LREQVDRVLLVTKTVLSAGSTAWAQGGIAAALDPEDSPQEHLEDTLVAGVDLCDESAV
                     HALVNEGPERVRELVALGAEFDRAPGGDILLTREGGHHRDRIAHAGGDATGAEISRAL
                     IEALERVKNDPGIEVIEHALVVDVLTDGADRVCGATLHVIGEGEQDGIGAAHARAVVL
                     ATGGLGQIYASTTNPSVATGDGMAAALRAGARLADLEFVQFHPTVMFLGEGSTGQQPL
                     ISEAVRGEGAFLVDDSGTRFMQGQHPQADLAPRDVVARAIVARMRETGAEHVWLDARH
                     LGKDFLEKRFPSIVARSRELGYDPATQLIPVAPAQHYASGGVWTDLDGRSTLDGLYAC
                     GETACTGVHGANRLASNSLLEGLVFAHRIAEDITARIQSGELAQVEPHAPDDDTSLLP
                     EECRLDVQRAMTAGSGVVRSAESTAQTYAAISHLLAATAPAQPTTAAWETTNLAHLGL
                     VLTAAAHARQETRGGHERIDYPERDDINWLGHLHHVHTPESVTMTFVPQEDSHR"
     gene            6389..7279
                     /gene="nadC"
                     /locus_tag="PKU75_06450"
     CDS             6389..7279
                     /gene="nadC"
                     /locus_tag="PKU75_06450"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPF80553.1"
                     /translation="MSDFEFPMDDARRVVHAALVEDLGGDDGVDVTTEATIPRDAVTT
                     GEIVARAPGVVAGLPVAQLVVDETATITGSGMCEVNILVPEGTVVARGDVLATVSGPT
                     ATILTAERTILNILSRISGVATATRDWADALEGTGATVLDTRKTTPGMRALQKYAVRA
                     GGGTNKRLGLFDVAMIKDNHKLAAGSITAAYDRIRERFPDVPVQVEVTTVEEGLEAVA
                     AGARFIMCDNMSPELLAETIEALSATGETIEVEATGGLTLERAAEYAATGVDYLSVGA
                     LTHSAAILDIALDLAEGQDV"
     gene            7471..8565
                     /locus_tag="PKU75_06455"
     CDS             7471..8565
                     /locus_tag="PKU75_06455"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HPF80554.1"
                     /translation="MKRRIATTIFGLALGVAGTQVGAHAEAPQDRGTTVVASGLNNPR
                     MISVGSDGALYVAEAGVGGSGPCITGAEGEACYGRTGAVTKIAGGKQTRVLSGLPSLA
                     GPGGFGASGPAGIAVSGSTYTLSYGLGAMPSARNDLPGMGRLLGTTVRGSFGSRATTI
                     GDIAAWEGVKDFDGAGPDSNPGGLALSGRTAVVTDAGANAVVRVTPRGKMSLLAVLPS
                     RLVPFGGGQIPMQSVPTAVAVGPDGAFYVSELTGFPFPAKAARIYRVVPGQRATIYAS
                     GLTNVTGLTWSRGRLYAVQFADDGLLAVPEGELPMGSLVEVRRDGNHRVVASGLTAPY
                     SVAVRGTDAYVTTCGLCAGLGGVSKVPLGG"
     gene            8602..9345
                     /locus_tag="PKU75_06460"
     CDS             8602..9345
                     /locus_tag="PKU75_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF80555.1"
                     /translation="MPRGQPDFLHPLPVRRVRMASDAEPVRDTWPASIPAVRQVLDQG
                     LDLAKLTILVGENGSGKSTLIEAIAMAYGLPGEGGSTGAMHKTYASESPLHDWLTLER
                     GAGASKWGYFLRAETTHGLFTYLHNNPGPVDPEFHALSHGESFLAMLGTRRFRGDGFF
                     VMDEPEAGLSFSAQLALVGTLKALASDPGSQVLIATHSPVLASIPEAHILQLDENGLR
                     PSAWAELESVINYTSFLKEPRRYLRHVVD"
     gene            9469..10923
                     /gene="lysS"
                     /locus_tag="PKU75_06465"
     CDS             9469..10923
                     /gene="lysS"
                     /locus_tag="PKU75_06465"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPF80556.1"
                     /translation="MRIRRAKRDRLLAEGRAPFAVHVPRTHSLQQVRDQWGQLETGEE
                     TEDVVGVAGRVVFIRNTGKLAFATLQEGIGTRLQVMLSLAEVGEEALAAWKADVDLGD
                     YVFVEGRVISSKRGELSVLAKRWEMASKALRPLPVLHKDLAEETRVRQRYADLIVRQE
                     ARDMVRTKATTLKAIREVLDGQGFVEVETPIIQLVHGGAAARPFTTHMNAFDQEMVLR
                     IALELNLKKAVVGGVDRVYEMGRIFRNEGVDATHSPEFTMLEVYQAWGDQTTIGQVMK
                     DLYLGVADALGSRQIDTPAGTVDLDGEWHWLPVYDAVSDAVRETVTIDTPVEKLREWA
                     VKFDVDHDPGLEKDKLFLELLSELVEPHLVQPTFLCDYPAIAQPLARPHRSRNGHIEA
                     WDLIIAGVERGTGFSELVDPVIQRAVLTDQSLRAAGGDPEAMQLDEDFLRALEYGAPP
                     MGGLGLGIDRMVMLFTGANIRETILFPHLKPEAH"
     gene            10923..11138
                     /locus_tag="PKU75_06470"
     CDS             10923..11138
                     /locus_tag="PKU75_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80557.1"
                     /translation="MDRLGLYAAALLPSIGVGYLFYIIIRNLLEGDRNERIALARIDK
                     ERERDTGQASPAADAGRTTRGDGTSSV"
     gene            11284..11604
                     /locus_tag="PKU75_06475"
     CDS             11284..11604
                     /locus_tag="PKU75_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HPF80558.1"
                     /translation="MAQRVQVMLIDDVDQTEASETVSFGLDGVTYEIDLSEGNAGKLR
                     DDLARWIGHARRTGGRKAAGTRAVGKADLAKVREWARENGHQVSDRGRISAVVQEAYQ
                     KAHG"
     gene            complement(11615..12511)
                     /gene="ligD"
                     /locus_tag="PKU75_06480"
     CDS             complement(11615..12511)
                     /gene="ligD"
                     /locus_tag="PKU75_06480"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HPF80559.1"
                     /translation="MVAGDPDVTRVEVAGRALKLSSLDKIMYPATETTKGEILNYYVQ
                     VAPVMLPHLGRRALTRVRWPHGVVGQNFFEKNLPSGAPSWLDRATIDTITYPVVNDLA
                     GLTYLVNLNSLEFHVPQWRVSGGGGRLNPDRLVIDLDPGKPAGLIECCVVALHVRERL
                     GELGMELFPVSSGSKGMQLYAALGGDLTSDEVRNLVSHLAQELTQRFPDLIVWKMTKA
                     LRPGKVLLDWSQNVAAKTTIAPYSLRGTEAPFVAMPRTWAEVEAAADGQSVIEQVLFD
                     DALATVARYGDRLAPLLRSVAG"
     gene            12573..13439
                     /locus_tag="PKU75_06485"
     CDS             12573..13439
                     /locus_tag="PKU75_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HPF80560.1"
                     /translation="MRAIWKGAVSFGLVNVPVRLYAATENHDVQFRQVHRTDGGRIKY
                     KRICSIDGEEVAYDDIAKGYETEDGDMVILTDEDFADLPTRSSKEIAVEKFVPADQID
                     PMLLDKCYYLEPDKASAKPYVLLRDALESEKRMAVVTVSIRTRMTMAVLRVKDGVIVM
                     QTMLWPDEVRNPDFASLGDEQEVSEKELAMAHLLVEQLAGDYDASEYEDDYAIALDTL
                     VKAKVEGGEIKDIPKPAEESGEVVDLLAALAKSVEKARAARGEAPAEEQPAAKKAASK
                     TTTKAAGKKKAG"
     gene            13440..14342
                     /locus_tag="PKU75_06490"
     CDS             13440..14342
                     /locus_tag="PKU75_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HPF80561.1"
                     /translation="MPEGHTLHRLAGALDHAFAGRPAVVTSPQGRFADGAALLTGHAV
                     EAAEAFGKHLFVRFADAPVLNVHLGLIGSFDVLPYAGGPPPPVGAVRVRMVGEQTYAD
                     LRGATLVQLVGPEQVEETIARLGPDPLRVGTPGNDPERSLARLARTSRPVAECLMDQS
                     IIAGVGNVYRAEVLFRHRIDPFVPGNKLKPKSWLAMWEDLVALLPYGVAFGQIITLDE
                     QLAEARELLDGRDPAAYDPSWGGVMPGERGDVVRGGDASPFEREYYVYKRDGLPCRVC
                     GSRVRTRVLAGRNLFWCGRCQRRH"
     gene            14376..15239
                     /locus_tag="PKU75_06495"
     CDS             14376..15239
                     /locus_tag="PKU75_06495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPF80562.1"
                     /translation="MRYAFVVPYADEPEFVELAVLGEQHGWDMVFTWEAVYRCDAWAT
                     LAAAAMRTERIRLGTLITPASRYRPWDLTQRVASVDRLSGGRVTLGVGLGALNQNWLA
                     FEPDEPRKQRAEKLDECLDIYAGLLGGESFSYDGRHWSVRPVTELAPPPPAQRPHPPV
                     WCVGALVPGRTRQPSLERAARWQGIFPSVAAGQDGNSGLTPDSFGEIVERLKMLREQA
                     GLPWEGYDVVVEGDSHGDFGGVHGDTEPWERAGATWWVESWWDIPEGAEGVAEVARRV
                     AVGPPRAAAAF"
     gene            complement(15236..15853)
                     /locus_tag="PKU75_06500"
     CDS             complement(15236..15853)
                     /locus_tag="PKU75_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF80563.1"
                     /translation="MTAASGTARPRDRKSAERRDQLAESALKTLGELGYARTSLRDIA
                     ANSDFSHGVVHYYFTDKTELITYCVRYYKAQCVTRYDEVIATSTTAAELAERFADKLC
                     ETVVDDGPMHRLWYDLRTQAMFEDVLRPDVLMIDATLEDMIWRVLTRYAELSDGELIV
                     DSPTAYAMLDGVFERSLLRHLSGDDEALTVLRTSAIELLPHFLRD"
     gene            complement(15961..16170)
                     /locus_tag="PKU75_06505"
     CDS             complement(15961..16170)
                     /locus_tag="PKU75_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80564.1"
                     /translation="MLGCLLAIACGLLAGATYLDLVGDETPAPVPVRQTATLAGIAVA
                     CSLAIAGLTVVATNSRIAPDQIRAE"
     gene            complement(16390..16878)
                     /locus_tag="PKU75_06510"
     CDS             complement(16390..16878)
                     /locus_tag="PKU75_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF80565.1"
                     /translation="MYATLGRLERDGLAELAEVEPGAGPERRLYRITADGVTRVDEWV
                     LTPQPPQEYAASSLHARVTIALLSGRDADRVLADQRALHLVRMRELLQQRRSATGREG
                     RQRLAVHDLTRPRCRRSVCCCLRTCRAWPRFSRTPRGTPEFERARDVSWSTSSTPPAS
                     RV"
     gene            17207..>17630
                     /locus_tag="PKU75_06515"
     CDS             17207..>17630
                     /locus_tag="PKU75_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPF80566.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGDGV
                     ASKALESLGIELENVREQVQEIIGTGQQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNKVRQQVIQL"
BASE COUNT         2943 a         6091 c         5988 g         2608 t
ORIGIN      
        1 caggctttcg acgcggccgg aaactcttct cccgcagtgc gaatcacctt caggacgaag
       61 gtgtcggccg agagccagcc gcccagcacc ccgacgaacc tcacggccga cccgacctcc
      121 gatgccgtca ccctgacctg gacccccagc accgacaacg tcaaggtcgc gcagtatgtc
      181 gtgaccgtcg gatccctgac cctcgccgtc accgaaccgc ggacgaccat caccgggctg
      241 acggcggaca ccgcataccg cgccaccgtg atggccaagg acacctccgg caacacctcg
      301 ggggaggcgg cggtggactt ccgcaccgtc accacggcgg acacgcaggc ccccactgcg
      361 ccgaccgcgc tgagcgcaac cccgaccacg aacgcagtaa cgctgacctg gacggcgagc
      421 accgacaatg tgggcgtcgt cggctacgac gtcacgatcg ggtcgacgac ccagacggtg
      481 accgggagcc cggccacagt caccgggttg agccccacaa cggcataccg cgcaacggtg
      541 gtcgccaagg atgcggccgg gcacgtctcg acccctgcgg tcatcgactt ccgcaccgcc
      601 acaccggcgg acacccaggc tccgacggcg ccgaccggcc tgtcggcgac cccgaccacc
      661 gacgcggtga cggtgaactg gacggcgagc accgacaacg tcggcgtcgc ggcctacgac
      721 gtcaccgtcg gcgacacaac gaagacggtg accgggacgt ccgcggcctt caccgggctg
      781 acgcccacca cggcataccg ggcgacggtg gtcgcgaagg atgcggcggg caatagctcg
      841 acgcccgcga cggtcgactt ccgcaccacc acgccgccgg atacccaggc accgacagcc
      901 cccacgaacc tgcgcgccac cgcggactcg acctcggcga gcctgacgtg gaccgccagc
      961 tcggacaatg tcggcgtcac cggatatgtg gtcagtgtgg gatcgatcac gcagaccgtg
     1021 accaccacgt cggcgaccgt cacccgcctg agcgctgcga cgacctacac cgcgcgtgtg
     1081 gtggccgagg atgccgccgg caaccagtcc accggggcga gcgtgacctt cacgaccgcg
     1141 accaacccga ccggcaaggt catctacgtc agccccgacg gctcggacgc caaccccggc
     1201 accgccggca gcccgaagcg caccatccag gccgccgtcg acctggcgga gccggggacg
     1261 gtcatccgcc tgtacgccgg gacctacgcc aacggcacca ccatccggat tcgcacctcg
     1321 ggccgcagcg acgcgccgat cgtcatccag cccgccggca acggcgaggt cacggtgaca
     1381 catccggtga cgaccgcgtc gtgcagcaac agcgcccccg catccgaccg gaccttcacc
     1441 ttcagcaatg gcgtcgacca ctggcgcatc gagaacctga acatccacaa cggggtctgg
     1501 atcgcgggca agcgctccaa ctacgcctac aactggctga cgaactatgt gaacaacggc
     1561 gactgggcat cccgccggcg cgccccgggt cacggcgaat acaaccccgc tgcagcccgc
     1621 accgacctcg tccccttcct gcgcgcacag acgggccaga gcgatctgga catggcggag
     1681 ggcatcacca tccggggcaa ccacctcacc gggcgcggcg tctacggtgc gctcaccagc
     1741 tatggggaga tcagcggcaa ccggatcgac cagattccgt gcgggatcgg cccgggcatc
     1801 tggctgatga ccttcagcaa cggctggaag atcttcgaca acgacgtctc cgatatcgcc
     1861 atctcgcacg cggcgcacta catgcaagag ggcatccgcg tcggcagcgc cgccaactac
     1921 aacgagatct acgacaatta cgtccacgac ctgcccggcg acgggcgcgg catcaacacc
     1981 gacgtcgacg gcagctggaa ccacatccac cacaaccggg tcgaaaacgt ggccatcggc
     2041 tacaacgacc agatgtccgg ctggggcaac cgctgggagt acaacacggt gaccaactac
     2101 cgggtctacg gcatgggatt ccggctcaag gacgcgacgt tggtgtcccc atcgctcgcg
     2161 agcagcacca acctcgtcaa ggtccggtgc aatgtcaccg ccaacccggt cggcgaccgg
     2221 gccaaggccc tcggtgtcgg cgcgagcatc gacggcacgt ggatcggcaa caccatgccg
     2281 aagatctggc tgagcaagaa cctgcgggtc tactggaccg cgcagggcaa cacgtggaac
     2341 ggctcctcgg cgctgcccgg ggactacccg ccgcaggaca cctcgatgtg ctgaggccgg
     2401 gcgctgccgc cgcggcggac accgaggatg attgactgag cgagtgacga ctccgccgct
     2461 gccgccggga cgcctgcgcg tgggcgtcgt gggggcgggc aaggtcggtg cggtcctcgg
     2521 tgccgccctg gcgaacgccg gacacctggt cgtttcggcg tgcgccgtca gcgaggcctc
     2581 ccgcgctcgc gccgcggcgt tgctgccgga tgctccgatc aggaccgtcg acgaggtcgt
     2641 cgacgaaagc gatctggtgc tgctggcggt gcccgatgac accctgtccg ggctggtgca
     2701 gggcttggcg acgaccgggg tatggcggct gggtcagctt gcgctgcata cctccggccg
     2761 cagcggcctc gcgccgttgg ctcccgcgat cgcgcagggg gtcggcggga tggcgatcca
     2821 tccggcgatg agtttcaccg gcacggcgat ggacgtcgaa cggctccgcg actgctgctt
     2881 cggcgtgacc gcccctcccg aactgatcgc gcccgcgtgt gcgctggtcg tggagatggg
     2941 cggcgagccg ctcgtcatcg acgagttcga ccgcgtgcgc tatcacctcg ggttggcgca
     3001 cagcgccaac tacctcgtca cccttgtcgc ccaggcggcg caagtgctcc agggcgccgg
     3061 ggtcgaggac accgaccggc tcctgcgccc gctgttgtcg gcagcgctgg acaacgccct
     3121 gcgccaaggt gattcggccc tcaccgggcc ggtcgcccgc ggcgatgccg gtaccgttgc
     3181 cgagcacctc gaggtgctcg aacgcgagac cgacgatgtg cgtgaggttt atcgcgtcct
     3241 tgccgaggcc accgtcgccc gcgccctgcg tggccggcga ctctcggagg ccggggcgcg
     3301 cagcatacgt gaggctctcg accgtccgaa ggagacaccg tgaccaccac aacggagcgc
     3361 cgcgctcccg ccgttgcgcg cacccgcgat gagctgcgag aggcgcgcaa agcgttgacc
     3421 ggcaaggaga ttgccgtcgt catgacgatg ggcgcgctgc acgacgggca cgcaaccctg
     3481 gtgaagcagg cgcgcgccga ggccgaccac gtcatcgtca cgatcttctt gaacccgctg
     3541 cagttcgggc ctcgggagga cctgtcgcgc tacccgcgga ccttcgactc cgacatggag
     3601 atctgcaccg agatgggtgc cgatctcgtc ttcgcgccga cgccggacgt catctatcag
     3661 gacggcgacc ccggcgttcg cgtctccgcc ggaccgctgg gagacgtgct cgagggccag
     3721 tcgcggcccg gccacttcga cgggatgctc accgtcgtcg cgaagctgat gcacctgacc
     3781 ggcggcaccg cgttcttctt cggacagaag gatgcccagc agttgctcct catccggcgg
     3841 atggtgcgcg acctcgactt ccccggccgc gtcatctccg tcccgaccgt ccgcgaggac
     3901 gacgggctcg cgatgtccag ccgcaacacc tacctgaccg agagcgaccg tgaggtcgcc
     3961 ctgagcctgt cgaaggcgct caaggcgggc gcggccgcgg cccccgaagg gccgtcggcg
     4021 atccggcgtg ccgcccgggc cgtgctcgtc gccgagccgc tcgtcctcgt cgactacctg
     4081 gtgctcgtcc acccgacgac gttgaaggat gtcccggagt ggtatcgcgg ggaggcattg
     4141 ctcgcggtcg cgggcaaggt cggcaccacg cggctgatcg acaatatgcc ggtgctcgtc
     4201 ggtcccggcg gtggcgccct gtcggttttc agcaatcccg acgacttggg ccccgcagag
     4261 gcggcgggcg gagcaaggta ggacgatgct gcgcaatatg ttgcactgca agattcatcg
     4321 cgcgacggtg acgcaggcgg atctgcacta tgtcgggtcg ctgacgatcg atcgggacct
     4381 gatggatgcc gctcacatgt tgcccggcca gcaggtcgac gtcgtcgatg tcgacaacgg
     4441 gcaccggctg acgacctatg cgatcgaagg ggagcgcggc agcggcatcg tctgcatcaa
     4501 cggcgcggcc gcccggctca tctcgccggg agacctcgtg atcatcatcg cgtacgccct
     4561 gatggacgag gaggaagcgc gcaccttcga accccaggtc gtcttcgtcg acaaacgcaa
     4621 ccggatcgtc gaggtcggcc acgacggcgg cgacgtcccc gacggatacg ggctcaagac
     4681 ctccgccgtg acgaagcggc gccatgcctg aactcgccac cttgccaccg gtccgcatac
     4741 cgcgctatct gctcgccggc gctcccgggt ggacgactcg agccgacgtc atcgtcgtcg
     4801 gttccggcat cgccggtctc acctgcgcgc tgcgcctgcg cgagcaggtc gatcgcgttc
     4861 tgctggtcac caagaccgtc ctgtccgccg gctcgaccgc ctgggcccag ggcggaatcg
     4921 ccgcggcact cgacccggag gactccccgc aggaacacct cgaggacacg ctcgttgccg
     4981 gcgtggacct gtgcgacgag tcggcagtgc atgcgctcgt caacgaggga cccgagcgcg
     5041 tgcgcgaact cgtcgctctc ggagcggaat tcgaccgcgc accgggcgga gacatcctgc
     5101 tcacccgcga aggcgggcat caccgggacc gcatcgcaca tgccggtgga gacgcgaccg
     5161 gtgcggagat ctcgcgcgca ctcatcgagg ctctggagcg ggtcaagaac gaccccggca
     5221 tcgaggtcat cgaacacgcc ctggtggtcg acgtgctcac cgacggtgcc gaccgggtgt
     5281 gcggcgccac cctgcacgtc atcggcgaag gtgagcagga cggcatcggt gccgcgcacg
     5341 ccagggcggt cgtcctcgcg acgggcggcc tgggccagat ctatgcgtcg acgacgaatc
     5401 cgtcggtcgc caccggagac ggtatggccg ccgccctccg cgccggcgcc cggctcgccg
     5461 acctggagtt cgtccagttc cacccgacgg tgatgttcct cggcgagggg tcgacgggac
     5521 agcagccctt gatctccgaa gcggtgcgcg gtgagggggc cttcctcgtc gacgactccg
     5581 ggacgcgatt catgcagggt cagcacccgc aggccgatct ggcgccacgc gacgtcgtcg
     5641 cgcgtgccat cgtcgcccgc atgcgtgaga ccggggccga acacgtgtgg ctcgacgccc
     5701 gccatctcgg aaaagacttc ttggagaagc gttttccctc gatcgtcgcg cggagccgcg
     5761 aactcggcta cgaccccgcg acccaactga tcccggtggc gccggcccag cactatgcct
     5821 ccggtggcgt ctggaccgac ctcgacggcc gctccaccct ggacgggctg tatgcctgcg
     5881 gcgagacggc gtgcaccggc gtccacggcg ccaaccggct ggcgtccaac tcgctgctcg
     5941 agggactcgt cttcgcccac cgcatcgcgg aggacatcac cgcccggatc cagtccggtg
     6001 aactggccca ggtcgaaccc cacgctcccg atgacgacac gagtctgttg ccggaggaat
     6061 gccgactgga cgtgcagcgg gcgatgacgg ctggatccgg agtcgtccgt tccgccgagt
     6121 cgaccgcgca gacgtatgcc gcgatcagcc acctgctcgc cgcgaccgcg ccggcgcagc
     6181 cgaccacggc ggcctgggag acgacgaacc tggcgcatct cggcctggtg ctgacagcgg
     6241 ccgcccacgc acgccaggag acccgcgggg gccacgagcg gatcgactac cccgagcgtg
     6301 acgacatcaa ctggctgggt cacctccacc atgtgcacac gcccgaatcg gtcaccatga
     6361 cctttgtccc gcaggaggat tcgcaccgat gagcgacttc gagttcccca tggacgatgc
     6421 ccggcgcgtg gtccacgcgg ccctggtcga agacctcggc ggtgatgacg gggtcgatgt
     6481 gacgaccgaa gccaccattc cacgggacgc cgtcaccacc ggcgagatcg tcgcgcgcgc
     6541 gcccggtgtg gtcgccgggc tcccggttgc ccaactcgtg gtcgacgaga ccgcgacgat
     6601 cacgggcagc ggaatgtgcg aggtgaatat ccttgtccca gaaggaactg tggtggcgcg
     6661 cggagatgtt ctcgctaccg tttccgggcc gacggcaacc atcctgacgg ccgagcgaac
     6721 catcctcaac atcctctccc gcatctccgg cgtcgcgacc gccacccggg actgggccga
     6781 cgcgctcgag ggcacgggcg ccaccgtcct ggacacgcgc aagacgacac ccgggatgcg
     6841 cgcgctgcag aaatacgccg tgcgcgccgg cggaggcacc aacaaacgcc tggggctctt
     6901 cgacgtcgcg atgatcaagg acaaccacaa actggcggcc ggctccatca ctgccgccta
     6961 cgaccgtatt cgcgaacgct tcccggacgt gccggtgcag gtcgaggtga ccacggtcga
     7021 ggaggggctc gaggccgtcg ccgccggcgc ccggttcatc atgtgcgaca acatgtcgcc
     7081 cgagttgctc gccgaaacca tcgaggcgtt gagcgcgacc ggagagacga tcgaggtgga
     7141 ggcgaccggt ggcttgaccc tggagcgcgc cgccgagtat gccgcgaccg gtgtcgacta
     7201 cctcagcgtg ggagcgctga cccattcggc cgccatcctc gacatcgcgc tcgacctggc
     7261 ggagggccag gacgtgtaac gaaaagccac cggcgggacg gggtccccgc gaagcaccgg
     7321 agcgacgaag gagcgaggac aggtcgtggg gtggcctagc ccggccaccg ggccggagcc
     7381 atcggctgct gccaggattt tgaggacttg cctttcccga gcgacaccgg cagagtgacc
     7441 agggtcacat tctgatcgga ggggtagtaa gtgaaacgac gaatcgccac aaccattttc
     7501 gggctcgctc tcggcgtcgc cgggacgcaa gtcggtgccc acgcggaggc gccgcaggat
     7561 agggggacga ccgtcgtcgc ctccggactg aacaacccgc ggatgatctc ggtgggcagc
     7621 gatggcgccc tctatgtcgc ggaggccggc gtcggaggtt ccggaccgtg catcaccggc
     7681 gctgagggtg aggcgtgcta cggccgcacc ggtgcggtca ccaagatcgc cggcggcaag
     7741 cagacccgtg tcctgagcgg cctgccctcc cttgccggtc ccggcggctt cggcgcatcc
     7801 ggacctgcgg gcatcgcggt cagcggatcc acctacaccc tcagctatgg cctcggtgcc
     7861 atgccgtcgg cgcgcaacga cctgccgggc atgggccggc tgctgggcac caccgtgcgc
     7921 ggcagtttcg ggtcgagggc cacgacgatc ggcgatatcg ccgcctggga gggtgtgaag
     7981 gacttcgacg gtgccgggcc ggacagcaac ccggggggct tggccctcag cggccggacc
     8041 gccgtggtca ccgacgcggg ggcgaacgcg gtcgtccgcg tgacgccccg gggcaagatg
     8101 tcgctgctcg cggtcctgcc gtcccggctg gtgccgttcg gcggtggcca gatcccgatg
     8161 cagtccgtgc ccaccgcggt ggcggtcggt ccagacggcg ccttctacgt ctccgaactg
     8221 acgggcttcc cgttcccggc gaaggccgcg cggatctacc gggtcgtccc cggccagcgg
     8281 gcgacgatct acgccagcgg cttgaccaat gtcaccggcc tgacctggtc tcgtgggcgg
     8341 ctgtatgccg tgcagtttgc cgacgatggc ctgctcgccg ttcccgaggg cgagttgccg
     8401 atgggttccc tcgtcgaagt gcgacgggac ggcaaccacc gggtcgtcgc gtccggtctc
     8461 accgccccct attcggtggc cgtgcggggc accgacgcct atgtcacgac gtgcgggctc
     8521 tgtgcggggc tgggcggcgt gagcaaggtg ccgctcggcg gctgagccgg gctgtcggtc
     8581 gcgcggcata cggtttgcgg tatgccgcgc ggccagcccg acttcctcca cccgttgccc
     8641 gtgcggcggg tgcggatggc gagcgacgcc gagccggtcc gggacacctg gccggccagc
     8701 atcccggcgg ttcggcaggt cctggaccag ggattggacc tggcgaagct gacgatcctc
     8761 gtcggcgaga acggcagcgg caagtccaca ctgatcgagg ccattgcgat ggcctacggc
     8821 cttcccggag agggcggctc gaccggtgcg atgcacaaga cgtatgccag tgaatcgcct
     8881 ctgcacgact ggctgaccct cgagcggggc gccggggcga gcaagtgggg ctacttcctc
     8941 cgggccgaga ccacccacgg actcttcacc tatctgcaca acaaccccgg ccccgtcgat
     9001 cccgagttcc acgctctcag tcacggcgag tccttcctgg cgatgctcgg cacgcgccgc
     9061 ttccgcggcg acggcttctt cgtcatggac gaacccgaag ccggcttgag cttctccgcg
     9121 caactcgccc tcgtcggcac cctcaaggcc ctggcgagcg atcccgggtc gcaagtgctc
     9181 atcgccaccc actccccggt gctggccagc atccccgagg cgcacattct gcaactggac
     9241 gagaacggcc tacggccgag cgcgtgggcc gagctggagt cggtgatcaa ctacaccagc
     9301 ttcctcaagg agccgcgccg ctacctgcgt cacgtggtgg actagcccac accaacgtct
     9361 tcgcgctgcg ctgcgccgaa acccggtggg gccgccgcgc cggcccacgt aagcttgtgc
     9421 gtcgtgaccg acgatcccac cgtgcccgag gccgaactgt ccgagcagat gcggatccgg
     9481 cgcgccaagc gggatcggct gctggccgaa ggccgcgcac cgttcgcggt gcacgtgccg
     9541 cgcacccact ccctgcagca ggtccgcgac caatggggtc agctggagac cggcgaggag
     9601 accgaggacg tcgtcggcgt tgccgggcgc gtggttttca tccgcaacac cggcaagctc
     9661 gccttcgcca cgctgcagga gggcatcggc acccgcctcc aggtcatgct cagcctcgcg
     9721 gaggtgggcg aggaggcgct cgctgcctgg aaggccgatg tcgatctcgg ggactatgtc
     9781 ttcgtcgagg gccgggtcat ctcctccaag cgaggtgagc tgtcggtgct cgccaagcgt
     9841 tgggagatgg cgagcaaggc cctgcggccc ctcccggtcc tgcacaagga cctcgccgag
     9901 gagacccggg tgcggcagcg ctacgccgac ctgatcgtgc gccaggaagc ccgcgacatg
     9961 gtccggacca aggcgacgac cctcaaggcg atccgggagg tcctcgacgg ccagggcttc
    10021 gtcgaggtcg agacccccat catccagctc gtccacgggg gcgcggccgc tcggccgttc
    10081 acgacccaca tgaacgcctt cgatcaggag atggtgctgc gcatcgcgct ggaactcaac
    10141 ctcaagaagg ccgtggtcgg tggggtggac cgcgtctacg agatggggcg gatcttccgc
    10201 aacgaaggtg tcgacgcgac ccattcgccg gagttcacca tgctcgaggt ctatcaggcc
    10261 tggggcgacc agaccacgat cgggcaggtc atgaaggacc tctacctcgg cgttgccgat
    10321 gccctcggct cacggcagat cgacacgccc gcgggcacgg tcgacctcga cggggagtgg
    10381 cactggctgc cggtgtacga cgccgtgtcg gacgcggtcc gcgagacggt gaccatcgac
    10441 acccccgtcg agaagctgcg cgaatgggcc gtgaagttcg acgtcgacca cgaccccggc
    10501 ctcgagaagg acaagctgtt cctcgaactg ctctccgaac tcgtcgagcc gcacctggtg
    10561 cagccgacct tcctctgcga ctaccccgcc atcgcccagc cgctcgcgcg gccgcaccgc
    10621 agccgcaacg gccacatcga ggcctgggac ctgatcatcg ccggcgtcga acgcgggacc
    10681 ggcttctccg aactcgtcga tccggtcatc cagcgtgcgg tcctgaccga ccagtcgttg
    10741 cgtgctgccg gcggcgaccc cgaagcgatg caactcgacg aggacttcct gcgggccctc
    10801 gaatacggcg cgccaccgat gggtggcctg gggctgggta tcgaccggat ggtcatgctc
    10861 tttaccggcg ccaacatccg cgagaccatt ctcttccccc atctgaagcc ggaggcgcac
    10921 tgatggaccg cttgggactg tatgctgcgg cgctgctgcc gtcgatcggt gtcggttatc
    10981 tgttctacat catcattcgc aatctcctcg agggtgaccg caatgagcgg atcgccctgg
    11041 cccgcatcga caaggagcga gaacgggata ccggtcaggc gtcaccggct gcggacgccg
    11101 gccggacaac ccgcggtgac gggacttctt cggtttagca atttcacgcc cgcggggcga
    11161 acccgggcgc cggctgtgaa atatgcaccg aaacctggca gccgtgtgcg caccccgcat
    11221 taagtgtgta ggctgggtgc tgccccgatt gggtatcaca cgttcaatgc ctaggagaat
    11281 gaaatggcgc aacgcgtcca ggttatgctc atcgacgacg ttgaccagac ggaagccagc
    11341 gaaacggtgt ccttcggtct cgacggcgtg acctacgaga tcgacctcag tgaaggcaat
    11401 gcgggcaaac tccgcgacga cctggcccgc tggatcggcc acgcccgccg caccggtggc
    11461 cgcaaggccg ccggcacgcg cgccgtgggc aaggcggatt tggccaaggt tcgcgaatgg
    11521 gcgcgggaga acgggcacca ggtcagtgac cgcggtcgca tctccgccgt cgtccaggag
    11581 gcctatcaga aggcccacgg ctgacctggt ttcgtcaacc ggccacgctg cggagcaacg
    11641 gagccaaccg atcgccgtag cgggccaccg tcgccagagc gtcatcgaac aggacttgtt
    11701 cgatgacgct ctgtccgtca gcggcggcct cgacctcagc ccacgtgcgc ggcatggcga
    11761 cgaagggggc ttcggtcccc cgtaacgagt agggcgcgat ggtcgttttc gccgccacat
    11821 tctggctcca gtccaaaagg accttgcccg gccgcaacgc tttggtcatc ttccacacga
    11881 tgaggtcagg gaatcgctgg gtgagttctt gcgccaggtg cgagaccagg ttgcgcacct
    11941 cgtcagaggt caagtcgccg ccgagcgcgg catacagttg cattcccttg ctccccgagg
    12001 acaccgggaa tagttccatc ccgagctcgc cgagcctttc gcgcacgtgt agcgcgacga
    12061 cacaacactc gatcaagccg gcgggcttgc caggatcgag atcgatgacc agccggtccg
    12121 gattcaaccg gcctccacca ccggaaaccc gccattgcgg gacgtggaac tccagtgagt
    12181 tgaggttgac caaataggtc aggccggcca gatcattaac cacggggtag gtgatcgtgt
    12241 cgatcgtcgc ccgatccagc caactcggcg cccccgacgg caggttcttc tcgaagaagt
    12301 tctgccccac caccccgtgc ggccaccgca cccgcgtcag cgcgcgtcga ccaaggtgcg
    12361 gcaacatcac cggagcgacc tggacgtaat agttgaggat ctcgcctttg gtcgtctcgg
    12421 tggccggata catgatcttg tccaggctcg acagcttcaa ggcgcggccg gccacctcga
    12481 cgcgagtcac gtcgggatcg ccggccacca tgggttcatt caacagcctc ggcccgacgg
    12541 cttgcgccgg cagttctgcc agacttgacg gcatgcgagc gatctggaag ggcgcggttt
    12601 ccttcggcct ggtcaacgtg ccggtccggc tgtatgccgc gaccgagaac cacgacgtgc
    12661 agttccggca ggtgcaccgc accgacggtg ggcggatcaa gtacaagcgg atctgctcca
    12721 tcgacggtga ggaggtcgcc tacgacgaca tcgccaaggg ctacgagacc gaggacggcg
    12781 acatggtcat cctcaccgac gaggacttcg ccgacctgcc gacgcgcagc tccaaggaga
    12841 tcgccgtcga gaagttcgtc cccgccgacc agatcgaccc gatgctgctc gacaagtgct
    12901 actacctcga gcctgacaag gcctcggcga aaccctatgt gctgctgcgc gatgcgctcg
    12961 agagcgagaa gcgcatggcc gtggtcaccg tctcgatccg cacccgcatg acgatggccg
    13021 ttctgcgggt caaggacggc gtcatcgtca tgcagacgat gctgtggccg gacgaggtcc
    13081 gcaaccccga cttcgcctcg ctcggcgacg agcaggaggt cagtgagaag gaactggcca
    13141 tggcccacct gctcgtcgaa caactggccg gcgactacga cgcgagcgaa tacgaggacg
    13201 actacgcgat cgccctggac accctcgtca aggccaaggt cgagggcggc gagatcaagg
    13261 acatccccaa gcccgcggag gagagcggcg aggtcgtcga cctgctcgcg gcgctcgcca
    13321 agtcggtcga gaaggccagg gcggcccgcg gcgaggctcc ggcggaggag cagccggcgg
    13381 ccaagaaggc ggccagcaag accacgacca aggccgccgg caagaagaag gccggctgag
    13441 tgccggaagg tcatacgttg caccggctcg ccggtgccct cgaccatgcc ttcgccggcc
    13501 gcccggccgt cgtcaccagc ccgcagggcc ggttcgccga cggcgcggca ctgctcaccg
    13561 ggcacgctgt ggaggccgcg gaggcgttcg gcaagcacct cttcgtgcgt ttcgccgacg
    13621 cgcccgtgct caatgtccac ctgggcctga tcgggtcctt cgacgtgctg ccgtatgccg
    13681 gtggcccgcc cccgcccgtc ggtgccgtcc gcgtgcggat ggtcggtgag cagacatatg
    13741 ccgatctgcg cggggccacg ctcgtgcagc tcgtcgggcc ggagcaggtc gaggagacca
    13801 tcgcccggct cgggccggac ccgctccggg tcgggacgcc cggcaacgac ccggaacgct
    13861 cgctggcccg gctggcgcgc acgtcgcgtc cggtcgcgga gtgcctgatg gaccaatcga
    13921 tcattgccgg cgtcggcaac gtctatcggg cggaggtgct cttccggcac cgcatcgacc
    13981 ccttcgtgcc cggcaacaaa ctgaagccga agtcctggct ggccatgtgg gaggacctgg
    14041 tcgcgctgct gccgtacggt gtggccttcg ggcagatcat caccctcgac gaacagctgg
    14101 ccgaggcccg ggaactgctc gacgggcgcg accccgcggc atacgacccg agctggggcg
    14161 gggtgatgcc gggcgaacgc ggcgacgtcg tgcgcggcgg cgacgcctcc cccttcgagc
    14221 gggagtacta cgtctacaaa cgcgacggac tgccgtgccg ggtgtgcgga tcccgggtgc
    14281 gcacgcgcgt cctcgccggg cgcaacctgt tctggtgcgg ccgctgtcag cgacggcatt
    14341 gagggctgtc gggcgcaccc ggtagtttcg cgagcatgcg ttacgccttc gtcgtcccgt
    14401 atgccgacga gcccgagttc gtcgaactgg ccgtgctggg ggagcagcat gggtgggaca
    14461 tggtcttcac gtgggaggcg gtgtatcgct gtgacgcgtg ggcgaccctc gccgccgccg
    14521 cgatgcgcac ggagcgcatc cggctcggca cgctcatcac tccggcgtcg cgctatcggc
    14581 cgtgggatct gacgcagcgg gtggcgagtg tcgaccggct gtccggtggg cgtgtcacgc
    14641 tcggtgtcgg gctgggggcg ctgaaccaga actggctggc tttcgagccc gacgagccgc
    14701 gcaagcagcg cgccgagaag ctcgacgagt gcctggacat ctatgcgggt ttgcttgggg
    14761 gagagtcttt ttcgtatgac gggcggcact ggtcggtgcg gccggtcacc gagctggcgc
    14821 caccgccgcc cgctcagcgg ccccacccgc ccgtctggtg cgtgggcgcg ctggtgcccg
    14881 gacgcacccg gcagccgtcc ttggagcgag cggcgcggtg gcaggggatc tttccctccg
    14941 tcgcggcggg acaggacggc aactccggtc tgacgccgga ttccttcggc gagatcgtcg
    15001 agcgactcaa gatgctccgc gagcaggccg gcttgccctg ggagggctat gacgtcgtgg
    15061 tggagggtga ttcgcacggc gacttcggcg gcgtgcatgg cgacaccgaa ccgtgggaac
    15121 gcgccggagc cacgtggtgg gtggagtcct ggtgggacat tcccgaggga gccgaaggcg
    15181 tcgccgaggt cgcccggcgc gtggccgtcg gccctccgcg cgcggcggcg gcgttctagt
    15241 cgcgcaggaa gtgggggagc agttcgatcg cgctggtgcg cagcacggtg agggcctcgt
    15301 cgtcgcccga gaggtggcgc agcagggagc gctcgaacac gccgtccagc atcgcgtagg
    15361 cggtcggtga gtcgacgatc agctcgccgt cggacagctc ggcatagcgc gtcagcaccc
    15421 gccagatcat gtcctccaag gtggcgtcga tcatcaacac gtcgggccgc agcacatcct
    15481 cgaacatcgc ctgggtgcgc aggtcatacc agagacgatg catcggcccg tcgtcgacga
    15541 ctgtctcgca cagcttgtcg gcgaaccgct ccgccagttc ggccgcggtc gtcgacgtgg
    15601 cgatcacctc gtcataccgg gtcacacact gggccttgta atagcgcacg cagtaggtga
    15661 tcagctcggt cttgtcggtg aagtagtagt ggacgacgcc gtgactgaag tcggagttgg
    15721 ccgcgatgtc acgcaggctc gtgcgggcat aacctagttc gccgagagtc ttgagtgcgg
    15781 actcagccag ctgatcacgg cgctcggcag acttgcgatc acgtggccgc gcagtccccg
    15841 atgcggcggt cataccagcg agtttaccgg cggaaacccc tgtccgtcct gctaagacag
    15901 ctgccaagaa ccggagcccg actgtggcgg tcaccacact ttctgacgct agcgccgggg
    15961 ttactccgcg cggatctggt cgggggcgat acgcgagttc gtggcgacga cggtcagccc
    16021 ggcgatggcc agggagcacg cgaccgcgat gccggcgagg gtcgcggtct ggcggacggg
    16081 caccggagcg ggtgtctcgt cgccgaccaa gtcgagatag gtcgcgcccg ccaggagtcc
    16141 gcaggcgatc gccaggaggc agccgagcac cgtcgggagc gcggcttcga gccactgggc
    16201 ccgtcgcagg gatgaccggc gaggagccga tgacctgcag ggccaccacc tcgcgccggc
    16261 tgacgagcgc tcggtcgatc gcggccatcg cgaaggtcaa caacccgatc ccgagaatga
    16321 ccgcggcgag cgtccagacg atggtccgaa gaccggcgac gaagtcgtag gtcgcgaggt
    16381 cgggccaggt cagacccgtg aagccggcgg cgtcgagctg gtcgaccaac tcacgtcccg
    16441 ggcccgctcg aactccggcg tgccacgcgg cgtcctcgag aaccggggcc aggcccggca
    16501 ggtccggagg cagcagcaca ctgacctcct gcaacggggc cgagtgaggt cgtggacggc
    16561 gagtcgttgc cggccttcgc ggccggtcgc ggagcggcgc tgctgcagca gctcgcgcat
    16621 ccgcaccagg tgtagcgccc gttggtcggc gagcacccgg tcggcgtccc ggcccgacaa
    16681 caaagcgatg gtgacccgtg cgtggaggga gctggcggca tactcctgtg gcggctgggg
    16741 cgtcagcacc cactcatcga cccgggtgac cccgtcggcg gtgatccgat agaggcgccg
    16801 ctccggcccg gcgcccggct cgacctcggc gagctcggcg agcccgtccc gctcgaggcg
    16861 acccaacgtg gcatacaccg cccgaaggcg aggggacgac gcagggcgaa atacgcgtca
    16921 taccgctgct tgagcgtgta gccgtgctgc gcctccggct ccaacagggc caggagggtg
    16981 ttcgccgtcg acatgcgagc gactatgcat cgagtgtgta cgccgaggga tcaccggcgc
    17041 gccgcgtgtg cgctgagagc gaacgcgagc gaccggcatg cggaccggtg gaacaagacg
    17101 gcccggctac gcagttgtca tcttcagttg cgtacccgaa acgactgcct gttgcccagg
    17161 gtgcgcggat accatcaagc tgactcgatc agcagggagt gcatttatgt tcgaacggtt
    17221 caccgaccgg gcccgacgcg tcgtggtgct cgcgcaggaa gaagcgcgca tgctcaacca
    17281 caactacatc ggcacggagc acattctgct cggcctcatt cacgagggcg acggggtggc
    17341 cagcaaggcc ctggagagcc tgggcatcga gctggagaac gtccgcgagc aggtgcagga
    17401 gatcatcggc acgggccagc aggcaccgtc cgggcacatc cccttcaccc cgcgcgcgaa
    17461 gaaggttctc gagctgagcc tgcgcgaagc cctccagctg ggccacaact acatcggcac
    17521 cgagcacatc ctgctcgggc tgatccgcga gggggagggc gtcgcggcgc aggtcctcgt
    17581 caagctcggt gccgacctga acaaggtgcg ccagcaggtc atccagctca
//
LOCUS       DAOPJG010000049        17606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3378_length_17606_cov_7.025697, whole genome shotgun sequence.
ACCESSION   DAOPJG010000049 DAOPJG010000000
VERSION     DAOPJG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17606
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3378_length_17606_cov_7.025697"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..826
                     /locus_tag="PKU75_06520"
     CDS             194..826
                     /locus_tag="PKU75_06520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPF80567.1"
                     /translation="MSGARLASWSYAEEFVPEAETADGARRTGSGLGAPPVGTGGGAA
                     LRMLAAASAAKAVIEIGTGAGTSGLWLLEGMPADGVLTTIDAAAEHSQAAKAAYAKAG
                     ISHTRTRVITGSAHDVLPRMTDGAYDMVFIDADKASYPEYVEHGIRLLRSGGVLALDN
                     MLWHDKVADPAARDAQTVALRELGKALRDDERLLPALLPVGDGLLVAVKK"
     gene            complement(962..1321)
                     /locus_tag="PKU75_06525"
     CDS             complement(962..1321)
                     /locus_tag="PKU75_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80568.1"
                     /translation="MDAGYGLAVRWSLTGAPLGVGQELRDYVISTSLARFMFLDGLAF
                     KTWRMREGEWFEGTYVFDSPRERDSFRAEFEATAAGSPVSRLIGSGPITIEDYEVVAI
                     AEGPAKFRRGAGPGSAR"
     gene            complement(1326..2816)
                     /locus_tag="PKU75_06530"
     CDS             complement(1326..2816)
                     /locus_tag="PKU75_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HPF80569.1"
                     /translation="MSPSALPPLPVIETQPLPIDAAAVLPADAAAAYAVLLAPDADWG
                     GAIAQAWPGAPAELAALIDAYGAPAAGKTTTIPLVGGPVARLILAAPAGEDGNAARSA
                     AAAAARAAGKASILLLQAPEPYAAAVVEGVVLAGPTVHLGPQTREPDDTRRALGRLIL
                     LGAPDPGQVRLGTECARAAQTARALANTPSNVKNPPWLANQARAIGRAAGLRVTVWDE
                     KALARDGFGGILAVGSGSVHEARLVMLEHRPGAATEPPIVLVGKGITFDTGGLNVKPA
                     AGMLMMKTDMSGSAIVLGVMAGLKALGVRRRVIGLLPLAENAMGAASYRPGDVVTHYG
                     GTTAEIGNTDAEGRIVLADALAYAAAELDPAALVDIATLTGHARIALASAMAPSYGTD
                     DALHTRIRDAFAAAGEPLWPMPLVEDYRSSLESDVADLSHIGPIGGQGAGSILAALFL
                     REFTGGRPWVHLDIAGTGRSESDTGFLAKGATGYGTRGLLRWLVEE"
     gene            complement(2931..3104)
                     /locus_tag="PKU75_06535"
     CDS             complement(2931..3104)
                     /locus_tag="PKU75_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HPF80570.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVLRMPLEGGGRLVVEMTPDEVR
                     ALGDAIANCEGLN"
     gene            complement(3245..3712)
                     /locus_tag="PKU75_06540"
     CDS             complement(3245..3712)
                     /locus_tag="PKU75_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80571.1"
                     /translation="MSAFRIVRRTGLDVEEAWAALTDFEAHGDVVPLTRLELDPGPPR
                     LGWGLHAGTGVGPIRLWDHMIVTIWSPPPRIDATPAEFRVVKIGRWLRGWAHVVIEPD
                     GSGSCVEWTEEVLPRFDPVPRLSDPLSRRAGERIFSRTVEGLLIRAEAADTLR"
     gene            complement(3719..4069)
                     /locus_tag="PKU75_06545"
     CDS             complement(3719..4069)
                     /locus_tag="PKU75_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPF80572.1"
                     /translation="MIWVFFTVVAVLLVAAFAGLIARRATYDSLPEPVHTAHDVQAAR
                     IERAGDIDHIHFDTALRGYRMDQVDAVLETLRTKLLAYEAGDRPTTRLSRVSPRVTPP
                     SAERSRIPTPPPGE"
     gene            complement(4066..4881)
                     /locus_tag="PKU75_06550"
     CDS             complement(4066..4881)
                     /locus_tag="PKU75_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPF80573.1"
                     /translation="MASVKRGHSPENVYHRGPATLRRSQVPNTTTDQRLLEGNDGASW
                     LHTDPWRVLRIQSEFVEGFGALAELGPAISVFGSARTPRDHPTYDRGVQVGRHIAEAG
                     YACITGGGPGVMEAANKGALEAGGVSVGLGIELPFETGLNEFVDLGINFRYFFVRKTM
                     FIKYAEGFVVLPGGMGTLDELFEAITLVQTQKISSFPIVLIGSDYWQGLIDWLGSTAL
                     GRGTIGEADLSLVQIVDDPAEAVALVVAGARHGQDPAVGQDGEEVHGTSEAES"
     gene            complement(4869..5954)
                     /gene="dapE"
                     /locus_tag="PKU75_06555"
     CDS             complement(4869..5954)
                     /gene="dapE"
                     /locus_tag="PKU75_06555"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HPF80574.1"
                     /translation="MPSLDLALDAVDLTAAVCDIESVSQDEAALADAIEAALRALPHL
                     HLTRIGNSVIARTEQGGAERIVLAGHIDTVPLTTDPPNLPTTRVTDAAGADILRGRGT
                     VDMKGGVAVMLRIAATVPQPNRDVTFVFYEAEEIDSQYNGLGLIEQQAPHLIDDAAFA
                     VLMEPTDAAVEGGCKGTLRAEITTKGIAAHSARPWKGHNAIHDAGEILRRLTAYEPQT
                     KIVDELEYREALNAVLIGGGTATNVIPDRCVVTVNYRYAPSLGPDEAEAHVREVFDGF
                     EIAIVDNAAGARPGMHLPAAKAFVAALDLPVSAKQGWTDVARFSAMGVPAVNFGPGDP
                     NLAHMDDEQCPTAQYTTCEEALVRWLR"
     gene            6118..7047
                     /gene="dapD"
                     /locus_tag="PKU75_06560"
     CDS             6118..7047
                     /gene="dapD"
                     /locus_tag="PKU75_06560"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HPF80575.1"
                     /translation="MTTSRYAWGFGLTTVTMDGTTLDAWYPDPQLGDHDEPPIPAEFE
                     AMRSEDPERGVRILPVAVGSDLDAPPATTADAYLRLHLLSHRLIEPNSINLDGIFAVL
                     PNVVWTNFGPCAVEGFEQTRMRLRARGPVIVYGIDKFPRMVDYVVPSGVRIADADRVR
                     LGAHLASGTTVMHEGFVNFNAGTLGTSMVEGRISQGVVVGDGSDIGGGASTMGTLSGG
                     GTERVSIGQRCLLGAESGLGIALGDDCVVEAGLYLTAGTKVTLPDGTIAKARELSGQS
                     NLLFIRNSVTGAVEARPRDGHGIVLNSALHAND"
     assembly_gap    7075..7174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7300..8193
                     /locus_tag="PKU75_06565"
     CDS             7300..8193
                     /locus_tag="PKU75_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80576.1"
                     /translation="MALITRQDTAITAAYPPPAHAPGRPSYRLRRTLVGVAVGALLAA
                     LIGLGLFTARWMTGPTCKATAGDMTFAFTPEQLRNASLITSVAIKRDLPPRAATIAIA
                     TAMQESELRNLDYGDRDSVGLFQQRPSQGWGSIEEIMDPLYAAGKFYDALVTIDDYED
                     RSITEVAQEVQRSAYPDAYADHEDQGRVLASALTGHSPAGIACRLGDAGTGDAAALAD
                     DLTSQLGHDAAVDGATVTVAAGSTQDAWAVAAWAAGQAERYAVTDVAAAGRAWHRARD
                     PLTWGPSAATADATTVTITVA"
     gene            complement(8710..10008)
                     /locus_tag="PKU75_06570"
     CDS             complement(8710..10008)
                     /locus_tag="PKU75_06570"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPF80577.1"
                     /translation="MADDATAGAKLLADGKELDLKRVSATEGNDGYDISALLKETGNV
                     TLDSGFVNTAACTSAITFIDGDTGILRYRGYPIEQLAEQSTYLETAYLLIYGELPSAD
                     EMASFDAKIRRHTLLHEDLKAFFNGFPRDAHPMPVLSSAVSALSTFYQDSLDPFDQDQ
                     VEISTYRLLAKLPTIAAYAYKKSMGQPFLYPDNHLSLTENFLRMTFGFPAEPYELDPT
                     IVKGLDLLLLLHADHEQNCSTSTVRLVGSSHANLFASISAGINALFGPLHGGANQQVL
                     EMLAQVKNSGESPKDFMMHVKNKETKQKLMGFGHRVYKNYDPRASIIKQTADDILGRL
                     GVDDPLLDIARELEEIALGDDYFVERRLYPNVDFYTGLIYKAMGFPTKMFTVLFALGR
                     LPGWIAQWREMIEDPATKIGRPRQVYIGEGARDYVAIDQR"
     gene            complement(10125..11264)
                     /gene="dapC"
                     /locus_tag="PKU75_06575"
     CDS             complement(10125..11264)
                     /gene="dapC"
                     /locus_tag="PKU75_06575"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HPF80578.1"
                     /translation="MSLRSLPDFPWDSLAPIKARASSHAGGLIDLSIGTPVDPTPQII
                     QDALCAAADAPGYPQTHGTPDLREAVAVWFEARRGVRHLDPAGVMPTIGSKELVAWLP
                     TLLDLGPGDVVAFPRVAYPTYDVGARLAGATPLAVDALTSLGPVTSKTAPKLLWLNTP
                     GNPNGKVLGVDHLRKVVEWARRHGVIVASDECYAELDWRDRSVGDWRHEPPTTPSLLD
                     PRVTGGTMDGMLVCYSLSKQSNLAGYRAAFLAGDPALVQRVLEVRKHAGFMVPWPVQR
                     ALVAALADPDHVAEIKARYAVRRATLLPAVESFGLRVDDSEAGLYLWATAGEDCWVTL
                     NRLADKGILAAAGAFYGPTGAQHVRIALTGTDAQIADAAGRLAGA"
     gene            complement(11261..11587)
                     /locus_tag="PKU75_06580"
     CDS             complement(11261..11587)
                     /locus_tag="PKU75_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HPF80579.1"
                     /translation="MTYVIAQPCVDLKDKACIEECPVDCIYEGERSLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDVPEQWADYYKANVEFFDDLGSPGGAAKMGMIPKDHPIIAALP
                     PQEHDE"
     gene            11626..12324
                     /locus_tag="PKU75_06585"
     CDS             11626..12324
                     /locus_tag="PKU75_06585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPF80580.1"
                     /translation="MTIASTPVSVPTALGTMPAHLWLPPAGRGPGIVLFQEIFGISAY
                     IEKRAADLAQAGYVVLAPEFYWRLGESGVADGPDMLEQGVNLMQRFDWEAGVRDGLAA
                     AGWLREHSAVTGRVGYVGFCFGGGLAFAVAAHSDPDALVSYYGSALPNLLDLAPQVTA
                     PSLHHFGDEDSYINPETVERIRAAVAGEQTEFYTYPEADHAFDNPDFVNHHPEASVIA
                     WGLTVKFLAKHLPV"
     gene            12321..13328
                     /locus_tag="PKU75_06590"
     CDS             12321..13328
                     /locus_tag="PKU75_06590"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80581.1"
                     /translation="MSRGTGMRATVRYRLDPPPPPGEPTLTDAVGEVLVNSDDEIVVE
                     TRRGVVRIPRDLVVATRIIPPKPQRRGVVGGSLAPLELHRAVADGWPAMTTDSLGDWL
                     LRASRGFTGRANSVLPVGDPGLPLIPALDAVEAWYAARHLPANLTIAGPVGFDPAADP
                     VGAAVLARGYVVSDPSLFLTADAAEVSRELSDAATQTVAAVGDSVSVELGDELTEVWL
                     AAYGGYRSGDREEIHAVMTGSPAQRFATARADGTVVGVGRLGVSGDWAGIAAMWVDPA
                     YRRRGLARAMMRELASAAPDLGASRLHLQVWAHNAPALALYERIGFRRHHAYVNAVTS
                     G"
     gene            complement(13301..13657)
                     /locus_tag="PKU75_06595"
     CDS             complement(13301..13657)
                     /locus_tag="PKU75_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF80582.1"
                     /translation="MTARKLAPPERSGGASCPVIEGVMGVLGRAWAGAVIEALLAGNT
                     RFTEIARYVGGATDAVLSARLKELCARGLVIRHVEPGPPVSVTYTLTEAGHDAAPVLE
                     AIRAYGQAHPEVTALT"
     gene            complement(13654..14301)
                     /locus_tag="PKU75_06600"
     CDS             complement(13654..14301)
                     /locus_tag="PKU75_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPF80583.1"
                     /translation="MTTITVVNAVLREPSSTRMLADRLAEAISAEVADAQIRHIDLRQ
                     HAHAITDALLTGFPSGELAEALASVRDADALVLVTPTFQGSYAGLFKSFADLVEPDAV
                     RGTPTMLAATGGSERHSLVIEHAMRPLAAYLGLLVVPTGVYAATADFGGPGAPELTRR
                     IHRAARELRTLVGGVTVAEAPEPAGSGHAEIDAGPLLGSFTPFDELLQSVSGAGR"
     gene            complement(14333..15508)
                     /locus_tag="PKU75_06605"
     CDS             complement(14333..15508)
                     /locus_tag="PKU75_06605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPF80584.1"
                     /translation="MQFGIFTVGDLTIDPVTGTTLTEHERIKATVAIAQKAEEVGLDV
                     FATGEHHNPPFAAPGNPPVLLAHLAAKTEKLILSTATTLITTNDPVHLAEDYAYAQHL
                     ADGRIDLMLGRGNTGPVYPWFGKDIRDGIDLAVENYALLHKLWREENVSFEGKYRTPL
                     YGFTSVPRPLDGIPPVVWHGSIRSPQIAEQAAFYGDAFFHNHIFWPKEHTERMVDLYR
                     RRFEHYGHGSADQAIVGLGGQVFMRANSQDAIREFRPYFDNAPVYGGGPSLESFMRET
                     PLTVGSPQQVIERTLGFREYVGDYQRQLFLIDHAGLPLQTVLEQLDILGGEVIPVLRK
                     EFAALKPGHVPDAPTHASLLAACRATATKETDQSYAAPVTDRWTGTTAETDNAPSAL"
     gene            complement(15597..16175)
                     /locus_tag="PKU75_06610"
     CDS             complement(15597..16175)
                     /locus_tag="PKU75_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016997035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HPF80585.1"
                     /translation="MGFLTPDASGELEVLRTYALQQLAQMRSTAYGLTDEQIRATPSV
                     SSLTIGGLLRHAGQVALHWSAAAAAAPGDPVIPEEFGEEQTIEECVAEATNLADTLDY
                     FDRCVATTDANIRSVTDLAAAVPVPDAPWFPPDLASWEARWVLAHITAEVARHTGHAD
                     IIRESIDGKGSYELNERADGFLDDDEEYAPHG"
     gene            16550..17467
                     /locus_tag="PKU75_06615"
     CDS             16550..17467
                     /locus_tag="PKU75_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPF80586.1"
                     /translation="MTVSETLRELGGFATYRALRQRHSRKELVQAHAAGAIVRESRGR
                     YAVPEVTSHAAIAHRYTAVQSHLSAALAHGWKVKTVPDRPWLLLTPGRRIPKGLRDEA
                     EYVCRATLSTHERKTGVTAPLRTVIDCARELPFDEALAVADSALRSKKVRREDLRAVA
                     AAARGHDADRIRTVVAAADGRAANPFESVLRAIVLEIDGLHVIPQAQIAEPGLFAVVD
                     LADRARKLVIEAEGYETHGTQSGFLKDVRRYSELAMYGWRVLRFTWIDVMFHPQWVRW
                     VIETWLAVDDGTQLPPQPDDSPGVYDRAS"
BASE COUNT         2593 a         6021 c         6228 g         2664 t
ORIGIN      
        1 cccagctggg cgccacccag gtctcggtgg ccgggctctc ggcctgcatc ttctcttcct
       61 cacgtgcgcg gttctcgcgc atccccctgt gcacactcat acgcgcgagt gggtttgatg
      121 gttccccaca agtctgaata gaagctgtat gcggcatatc agcgcggtct cgactgggac
      181 gtaaaatcca ggcatgagtg gagcgagact ggcgagctgg tcgtatgccg aggagttcgt
      241 ccccgaggcc gagaccgcgg acggggcccg gcgcaccggt tcggggctgg gggcgccgcc
      301 ggtcggcacc ggcggcggcg ctgccctgcg gatgctcgcc gccgcgagcg ccgcgaaggc
      361 ggtcatcgag atcggcacgg gcgcgggcac ctccggcctg tggctcctcg agggtatgcc
      421 ggccgacgga gtgctgacca cgatcgacgc cgcggccgaa cactcccagg cggcgaaggc
      481 ggcctacgcg aaggcgggca tctcgcacac ccgcacccgc gtcatcaccg ggtccgccca
      541 cgatgtcctg ccgcggatga ccgacggcgc ctacgacatg gtgttcatcg acgcggacaa
      601 ggcgagctac ccggagtatg tcgagcacgg catacggctg cttcgctccg gcggcgtcct
      661 ggcgctggac aacatgctct ggcacgacaa ggtcgccgac cccgccgcgc gcgacgccca
      721 gacggtggcc ctgcgcgaac tcggcaaggc cctgcgcgac gacgagcgcc tgctgccggc
      781 cctgttgccg gtgggtgacg gcctgctggt cgcggtcaag aaataggccg ggccaggcgg
      841 agccaccagg gcagggagtt caccggtagc cagcggtcgt gctccgccag aacgacgcga
      901 ctacccattg ggatctgggg ctttcacccc actgaccaag ggttaaccgg gtggctaccg
      961 gtcagcgggc ggagccgggg ccggcgcccc ggcggaactt tgccgggccc tcggcgatgg
     1021 cgacgacctc gtagtcctcg atcgtgatcg gcccggagcc gatgaggcgc gagaccgggg
     1081 acccggcggc ggtggcctcg aactcggccc ggaagctgtc ccgctcgcgc ggggagtcga
     1141 agacgtaggt gccctcgaac cactccccct cgcgcatccg ccaggtcttg aaggcgagcc
     1201 cgtcgaggaa catgaaccgg gccagcgaag tgctgatgac gtaatcccgc agttcctgcc
     1261 ccacaccgag gggcgcgccg gtcaacgacc agcgcaccgc cagcccgtag cccgcgtcca
     1321 tacctctact cctcgaccag ccagcgcaac aatccgcggg taccgtagcc ggtcgcgccc
     1381 ttggcgagga atccagtgtc ggactccgaa cgccccgtgc cggcgatgtc gaggtgcacc
     1441 cacgggcgcc cgcccgtgaa ctcgcggagg aagagggccg cgaggatcga tccggcaccc
     1501 tgtccgccga tcgggccgat gtggctcagg tcggcgacat cggactcgag cgaggagcga
     1561 tagtcctcca ccagcggcat cggccacagc ggctcgccgg ccgccgcgaa ggcgtcccgg
     1621 atccgcgtat gcaatgcgtc gtcggtgccg tatgacggtg ccatcgcgct cgccagggcg
     1681 atgcgggcgt ggccggtcag ggtggcgatg tcgacgaggg cggcagggtc gagttcggcg
     1741 gcggcgtatg ccagcgcgtc ggccaggacg atgcgcccct cggcgtccgt gttgccgatc
     1801 tcggcggtcg tgccgccgta gtgggtgacg acatcgccgg gccgatagga cgcggcaccc
     1861 atcgcgttct cggccagcgg cagcaaccca atcacccggc gacgcacgcc cagcgccttc
     1921 agccccgcca tcacgccgag gacgatcgcg gagccggaca tgtcggtctt catcatcaac
     1981 atgccagcgg ccggtttgac gttgaggcca ccggtgtcaa aggtgattcc cttgcccacc
     2041 aacacgatcg gcggctcggt ggcggcaccg gggcggtgct cgagcatcac gaggcgggcc
     2101 tcgtgcaccg aaccggagcc gacggcgaga atgcctccga atccgtcccg cgcgagcgcc
     2161 ttctcgtccc acaccgtcac gcgcagtccg gcggcgcggc cgatcgcgcg cgcctggttg
     2221 gccagccacg gtgggttctt gacattgcta ggggtgttgg cgagtgcccg ggccgtctgg
     2281 gccgcgcgag cgcattcggt gccgaggcgc acctgccccg ggtccggcgc accgaggagg
     2341 atcagccggc ccagggcgcg gcgggtgtcg tccggctcgc gggtctgcgg ccccaggtgg
     2401 acggtgggcc cggcgagcac gaccccttcg acgacggcgg cggcatacgg ctcgggggcc
     2461 tgcaggagga ggatggacgc cttccccgcc gcccgggccg cagcggccgc cgcggaccgc
     2521 gcggcgttcc cgtcctcccc ggccggcgcc gccaggataa ggcgcgccac cgggccgccc
     2581 accagcggga tcgtcgtggt cttgccggcc gccggcgcgc catacgcgtc gatcagcgcc
     2641 gcgagttcag ccggcgcacc gggccacgcc tgggcgatgg caccacccca gtcggcatcg
     2701 ggggcgagca ggacggcgta ggcggccgcg gcgtcggccg gcaggacggc ggcggcgtcg
     2761 atcggcagcg gctgggtctc gatgaccggc aggggcggga gcgcggaggg ggacatgatc
     2821 taccaaccct aaccaccccc ggcacgacaa gcgccccgga cgaccgtggt cgtcaggggc
     2881 gctgaccggc gggagatcga ctcccgcagt ggatcctggt gccggcgggg ctagttgagg
     2941 ccctcgcagt tggcgatggc gtcgccgagg gcgcgtacct cgtcgggcgt catctcgacg
     3001 acgagacggc cgcccccttc caggggcatg cgcagcacga tgccgcgacc ttccttggtc
     3061 acttccaaag gcccgtcgcc ggtccgaggc ttcattgccg ccatgggcgc aatcccttct
     3121 tgtccacgag gtcgtgctcc ggaagctccg gtcgtcccgt gttgtaatcc aggaccggac
     3181 acgtgcccgg ccatccgtcc cattatcccc cacgcttgcg gcagagcgga aatcgccccc
     3241 ctgctcagcg gagcgtgtcg gcggcctcgg cccggataag cagcccctcg acggtgcggg
     3301 agaagatccg ctcgccggcg cggcgggaga gcgggtcgct caggcgcggc acggggtcga
     3361 atcgtgggag cacctcctcg gtccactcga cgcacgagcc gctcccgtcg ggttcgatga
     3421 ccacgtgggc ccagccccgc agccaccggc cgatcttgac gacgcggaac tccgccgggg
     3481 tcgcgtcgat ccgagggggc ggcgaccaga tcgtgacgat catgtggtcc cacaggcgaa
     3541 ttgggccgac gccggtgccg gcgtggaggc cccagccgag gcgcggcggg ccggggtcga
     3601 gttcgagccg ggtcagcggg accacgtcgc cgtgggcctc gaagtcggtc agggccgccc
     3661 aggcttcctc gacgtcgagt ccggtgcgac gcacgatccg gaaggcgctc acaagcgtct
     3721 actcccccgg cgggggcgtc ggaatccggc tgcgctctgc cgacggcggc gtgacgcgcg
     3781 gtgagacccg gctaagccgc gtcgtcggcc ggtcgcccgc ctcatacgcc aggagtttgg
     3841 tgcgcagcgt ctccagcacc gcgtcgacct ggtccatccg gtagccgcgc agcgcggtgt
     3901 cgaagtggat gtggtcgatg tcgccggcgc gttcgatccg agcggcctgc acgtcgtggg
     3961 cggtgtgcac cggctcgggc agcgagtcgt aggtcgcgcg gcgggcgatg aggccggcga
     4021 aggcggccac cagcagcacc gcgacgaccg tgaagaagac ccagatcatg actccgcctc
     4081 cgaggttccg tggacttcct cgccatcctg ccccacggcg gggtcctggc cgtggcgggc
     4141 gccggcgaca accagcgcga cggcctcggc cgggtcgtcg acgatctgca ccagcgacag
     4201 atcggcctcg ccgatcgtgc cgcggcccag cgccgtcgaa ccgagccagt cgatcagccc
     4261 ctgccagtag tccgaaccga tcagcacgat cgggaaggag gagatcttct gcgtctggac
     4321 caaggtgatg gcctcgaaaa gctcgtcgag tgtgcccatg ccgccgggca ggacgacgaa
     4381 tccctccgca tatttgatga acatcgtctt gcgcacgaag aagtagcgga agttgatgcc
     4441 aaggtcgacg aactcgttca gcccggtctc gaagggcagt tcgatgccga gcccgacgga
     4501 caccccgccc gcctcgagcg cgcccttgtt ggcggcttcc atcaccccgg gcccgccgcc
     4561 ggtgatgcac gcgtagcccg cctcggcgat atgccgcccg acctggaccc cacggtcgta
     4621 tgtcgggtgg tcgcgcgggg tgcgcgcgga gccgaagacg gagatggcgg ggccgagttc
     4681 ggcgagcgcc ccgaagcctt cgacgaactc gctctggatc cgcaagaccc gccaggggtc
     4741 ggtatgcagc cagctcgccc cgtcgttgcc ctccagcaat cgctggtcgg tggtggtgtt
     4801 ggggacctgg gagcgccgca gcgtcgccgg accacggtgg tagacgttct cgggcgagtg
     4861 cccgcgcttc accgaagcca tcggaccagt gcctcctcgc aggtggtgta ctgcgcggtg
     4921 gggcactgct cgtcgtccat gtgggcgagg ttggggtcgc ccggcccgaa gttgacggcg
     4981 ggcacgccca tcgcggagaa gcgcgcgacg tcggtccacc cctgtttggc gctgacgggc
     5041 aggtcgagcg cggcgacgaa ggccttggcg gccggcaggt gcatgccggg ccgggcgccc
     5101 gcggcgttgt cgacgatggc gatctcgaag ccgtcgaaca cctcgcgcac gtgcgcctcg
     5161 gcctcgtcgg gcccgaggga aggggcgtag cggtagttga cggtcacgac gcaccggtcg
     5221 gggatgacat tggtggccgt gccgccgccg atgaggacgg cattgagggc ctcgcggtat
     5281 tcgagttcgt cgacgatctt ggtttgcggc tcgtatgccg tgagccgccg caggatctcg
     5341 ccggcgtcgt ggatcgcgtt gtgccccttc cacggccggg cggagtgggc ggcgatgccc
     5401 ttggtggtga tctcggcccg cagcgtgccc ttgcagccgc cctcgacggc ggcgtcggtg
     5461 ggctccatca ggacggcgaa ggcggcgtcg tcgatcaggt gtggggcctg ctgctcgatc
     5521 aggccgaggc cgttgtactg gctgtcgatc tcctcggcct cgtagaagac gaaggtgacg
     5581 tcgcggttgg gctgcgggac ggtggcggcg atgcgcagca tgaccgcgac gccgcccttc
     5641 atgtcgaccg tgccgcggcc gcgcaggatg tcggcgccgg cagcgtcggt gacgcgcgtg
     5701 gtcggcaggt tgggcgggtc ggtcgtcagc ggcacggtgt cgatgtggcc ggcgaggacg
     5761 atccgctccg ccccgccctg ctccgtgcgg gcaatgacgc tgttgccgat ccgggtcagg
     5821 tgcaggtggg gcagggcgcg cagggcggcc tcgatggcgt cggccagggc cgcctcgtcc
     5881 tggctcaccg actcgatgtc gcacacggca gccgtcaggt cgaccgcatc gagcgcaagg
     5941 tccagggatg gcatggaccg aaccatagtc gccccgggcg aacgcaaagg tccaccttcg
     6001 ccgggccggg ttgcgcgagc ggggccaggt tgtgcgcgag cggggccggt ggaggccgcg
     6061 tgcaggtcgc tgtgttggag cgcagcgtgt cccggcccgc aacctacgat gtgccccatg
     6121 acgacttccc gctacgcctg gggtttcggg ctgaccaccg tgaccatgga cggcaccact
     6181 ctcgacgcct ggtatccgga cccgcagctc ggcgaccacg acgaaccccc catcccggcg
     6241 gagttcgagg cgatgcggtc cgaggacccc gagcggggcg tgcggatcct gcccgtggcc
     6301 gtcggctccg acctcgatgc gccgccggcc acgaccgccg acgcctacct gcgcctgcac
     6361 ctgctcagcc accgcctgat cgagcccaac tcgatcaacc tcgacggcat cttcgcggtg
     6421 ttgcccaacg tggtgtggac caatttcggg ccgtgcgcgg tggagggttt cgagcagacc
     6481 cggatgcggc tgcgtgcccg gggcccggtg atcgtctacg gcatcgacaa gttcccccgc
     6541 atggtcgact acgtcgtccc gtccggcgtc cgcatcgccg acgccgaccg ggtgcgcttg
     6601 ggcgcacacc tggccagcgg caccacggtc atgcacgagg gcttcgtcaa cttcaacgcc
     6661 ggcaccctcg gcacctcgat ggtggagggc cgcatcagcc agggcgtcgt cgtcggcgac
     6721 ggctcggaca tcggcggcgg cgcctcgacg atgggcaccc tctccggcgg cggcaccgag
     6781 cgggtctcga tcgggcagcg ttgcctgctc ggggccgagt ccggactcgg catcgcgctc
     6841 ggcgacgact gcgtcgtcga ggcggggctc tatctgaccg ccggcaccaa agtcactctg
     6901 cccgacggaa ccatcgccaa ggcccgcgag ttgtccggtc agagcaacct gctcttcatc
     6961 cgcaactcgg tcaccggtgc cgtcgaggcc cggccgcgcg acggccacgg catcgtgctc
     7021 aacagcgctc tgcacgccaa cgactgacac cccgcgaggt tctcttccgc gccgnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaacag cgctctgcac gccaacgact
     7201 gacaccccgc gaggttctct tccgcgccgc gcgtcgctcc gactcctcgc gtggacccga
     7261 acactccaac cacaaagacc gccgacagcc actcatctca tggcactcat cacacggcag
     7321 gacacagcca tcaccgccgc atacccgcct ccggcgcacg cacccggccg gccgtcatac
     7381 cggctgcgcc gcaccctcgt cggggtcgcg gtcggcgcgc tgctcgccgc cctgatcggt
     7441 ctcgggctct tcaccgcccg ctggatgacc gggcccacgt gcaaggcgac cgccggcgac
     7501 atgaccttcg cgttcacccc ggagcagctg cgcaacgcct ccctgatcac ctcggtcgcg
     7561 atcaagcgcg acctgccgcc gcgggcggcg acgatcgcga tcgcgaccgc catgcaggaa
     7621 tcggagttgc gcaatctcga ctacggcgac cgtgattccg tcggcctctt ccagcagcgg
     7681 ccctcccagg ggtggggcag catcgaggag atcatggacc ccctctatgc ggcgggaaag
     7741 ttctacgacg ccctcgtcac catcgacgac tacgaggacc ggtcgatcac cgaggtggcg
     7801 caggaggtcc agcgcagcgc ctaccccgat gcgtatgccg accacgagga ccaggggcgc
     7861 gtgctcgcct cggcgctgac cggccattcc cccgccggca tcgcctgccg gctcggcgac
     7921 gccggcacgg gcgacgccgc ggccctcgcc gacgacctga ccagccaact cggtcacgac
     7981 gcggccgttg acggggccac cgtcacggtg gcggccggca gcacccagga cgcctgggcc
     8041 gtggccgcct gggccgccgg tcaggccgag cgctacgcgg tcacggacgt cgctgctgcc
     8101 ggccgcgcct ggcaccgagc ccgggacccg ctgacgtggg ggcccagcgc cgcgaccgcc
     8161 gacgccacga cggtgacgat caccgtggcc tgacggcagc ggggtcggag ggacgagccg
     8221 ggcggcacgc ccaggcacac cacgcgcccg tagggcggcc cggagaccgt gacgacgatg
     8281 cgcccgcgcg cccgtgtcgg gccggcttgc ggaaaaccgg gaatcctgac ggtgcgcagc
     8341 gtgccaccgc acgccaggga gaggatcgag acgaccgttt cgaaggtccg gacacggttg
     8401 atgaccttgg tgtggatcag caacccaacg ccggccgcca tctcgggatg gtaggggcgt
     8461 tcgagccgtc gccggcgaaa ggagacgttc gacgcggttc ctcagatggt cgtcatagtc
     8521 gtatgcctgt gaccagtccg cagccgtgac cggcggcccc ggccacgtga ccgcctcggc
     8581 aggtgcccgc accgaccagt gctcgtcttg tccgcatacc gaaacccacc gggtcgatgg
     8641 ccgggccgat cccggccgtg accacacggt atgccgcgcg atgccccgtt ggctagggac
     8701 gcacggcttt cagcgctggt cgatggcgac gtagtcgcgc gcgccttcgc cgatgtagac
     8761 ctgccgcggg cggccgatct tcgtcgccgg gtcctcgatc atctcgcgcc actgcgcgat
     8821 ccacccgggc aggcggccga gggcgaacag cacggtgaac atcttggtcg ggaaacccat
     8881 cgccttgtag atcaggccgg tgtagaagtc gacgttcggg tagaggcggc gctcgacgaa
     8941 gtaatcgtcg cccagggcga tctcctccag ttccctcgcg atgtcgagca gcgggtcgtc
     9001 gacaccgagc cgccccagga tgtcgtcggc ggtctgcttg atgatcgagg ccctagggtc
     9061 gtagttcttg tagacccggt gcccgaagcc catcagcttc tgcttggtct ccttgttctt
     9121 cacgtgcatc atgaagtcct tgggggactc gccggagttc ttgacctgag cgagcatctc
     9181 caagacctgc tggttggcgc ccccgtgcag cggcccgaag agggcgttga tgccggccga
     9241 gatcgacgcg aacaggttgg cgtgcgacga gccgacgagg cgcaccgtcg aggtggagca
     9301 gttctgctcg tggtcggcgt gcaggagcaa cagcagatcg aggcccttga cgatggtcgg
     9361 gtcgagttcg tagggctcgg ccgggaagcc gaaggtcatc cgcaggaagt tctcggtcag
     9421 cgacaggtgg ttgtccgggt agaggaaggg ctggcccatc gacttcttgt aagcgtaggc
     9481 cgcgatcgtc ggcagcttgg ccagtagccg gtaggtcgag atctcgacct ggtcctggtc
     9541 gaaggggtcg aggctgtcct ggtagaaggt cgacagggcc gagacggcgc tggagagcac
     9601 cggcatcggg tgcgcgtcgc gcgggaagcc gttgaagaag gccttgaggt cttcgtgcag
     9661 gagggtgtgc cgccggatct tggcgtcgaa ggaggccatc tcgtccgcgc tgggcagctc
     9721 cccgtagatc agcaggtatg ccgtttcgag ataggtggat tgttcggcca gctgctcgat
     9781 cgggtacccg cggtagcgca ggatgcctgt gtcaccatcg atgaacgtga tcgcgctggt
     9841 gcaggcggcg gtattgacga agccggagtc gagggtgaca tttccggtct ccttgagcag
     9901 ggcggagatg tcgtatccgt cgttgccctc ggtggcggat acccgcttga ggtcgagttc
     9961 cttgccgtcc gcgaggagtt tcgcaccagc cgttgcgtcg tctgccatgg ggtggaaact
    10021 accgcacgag aaccgcgagg gtggcacgcg gtccgcgaaa tctgcgtcct tgaccggcgc
    10081 ccggcaccgc acccgggacc cgtcggcggg tccagcgcgc gggctcaggc gccggcgagc
    10141 cgtccggcag cgtcggcgat ctgggcgtcg gtgccggtca gcgcgatccg cacgtgctgg
    10201 gcgccggtgg ggccgtagaa ggcgccggcc gccgcgagaa ttcccttgtc cgccaaccgg
    10261 ttcagggtga cccagcagtc ctcgcccgcg gtcgcccaca gatagagccc ggcctcggag
    10321 tcgtcgacgc gcagcccgaa cgactccacg gcgggcagca gagtggcccg gcgcacggca
    10381 taccgagctt tgatctcggc gacatggtcg gggtcggcga gcgcggcgac cagcgctcgt
    10441 tgcaccggcc acggcaccat gaagcccgcg tgcttgcgca cctccagcac ccgctgcacg
    10501 agcgcgggat cgccggcgag gaaggccgcg cgatagccgg cgagattgga ctgcttggac
    10561 agggagtagc agaccagcat cccgtccatc gtcccgccgg tgacgcgcgg gtcgagcagc
    10621 gacggggtcg tcggcggctc gtggcgccag tcccccaccg accggtcgcg ccagtcgagc
    10681 tcggcatagc actcgtcgga ggcgacgatc acgccgtgcc ggcgcgccca ttcgacgacc
    10741 ttgcgcaggt gatcgacgcc gagcaccttg ccgttggggt tgccaggagt gttgagccac
    10801 aacagtttcg gcgccgtctt gctcgtgacc gggccgaggg aggtcaacgc gtccacagcg
    10861 agcggggtgg cgccggcgag gcgggcgccc acgtcatacg tcggataggc gacgcggggg
    10921 aaggcgacca catcccccgg cccgaggtcg aggagggtgg ggagccaggc gacgagttcc
    10981 ttggaaccga tcgtcggcat gacgccggcc gggtcgaggt ggcgcacgcc gcgccgcgct
    11041 tcgaaccaca ccgccaccgc ctcgcgcaga tcgggggtgc cgtgggtctg cggatagccc
    11101 ggcgcgtcgg cggccgcgca gagggcgtcc tggatgatct gcggcgtcgg gtcgaccggc
    11161 gtgccgatcg agaggtcgat caggcccccc gcgtgggaac tcgcccgcgc cttgatcggc
    11221 gcgagcgagt cccagggaaa gtcaggcagt gagcgcaggc tcactcgtcg tgctcctgcg
    11281 gcggcagcgc cgcgatgatc gggtggtcct tgggaatcat gcccatcttg gccgccccgc
    11341 ccgggctgcc caggtcgtcg aagaactcga cattggcctt gtaatagtcc gcccactgct
    11401 cgggcacgtc gtcctcgtaa tagatcgcct cgaccgggca caccggctcg cacgcaccgc
    11461 agtccacaca ctcgtcgggg tggatgtaga gactgcgctc gccctcgtag atgcagtcga
    11521 ccggacactc ctcgatgcag gccttgtcct tgaggtccac acacggctgg gcgatcacat
    11581 acgtcatggc ggcattctat gcaggccagg catacgctgc ggcccatgac cattgcatcc
    11641 accccggtgt ccgtcccgac cgcgctgggc accatgcccg cccacctctg gctgccgccg
    11701 gccggccgcg gccccgggat cgtgctcttc caggagatct tcgggatcag cgcctacatc
    11761 gagaagcgcg ccgccgatct ggcgcaggcg gggtatgtcg tgctggcgcc ggagttctat
    11821 tggcgcctgg gcgaatccgg cgtggccgac gggccggaca tgctcgagca gggtgtcaac
    11881 ctgatgcagc gtttcgactg ggaggccggg gtgcgggacg gcttggcggc cgccggctgg
    11941 ctgcgcgagc attccgctgt cacgggccgg gtcggctatg tgggcttctg tttcggtggc
    12001 gggctcgcgt tcgcggtggc cgcgcacagc gaccccgatg ccctcgtcag ctactacgga
    12061 tcggccctgc cgaacctgct cgacctggcc ccccaggtga cggccccgag cctgcaccat
    12121 ttcggcgacg aggactccta tatcaacccg gagacggtcg aacgcatccg ggccgccgtg
    12181 gccggcgagc agaccgaatt ttacacctat cccgaggccg atcacgcctt cgacaacccc
    12241 gacttcgtca accaccaccc ggaggcgtcg gtcatcgcgt gggggctcac ggtgaagttc
    12301 ctcgcgaagc acctgccggt gtgagccgcg ggaccggtat gcgtgccacg gttcgctacc
    12361 gcctcgaccc gccacctccc cccggcgagc ccacactgac cgacgcggtg ggcgaggtgc
    12421 tcgtcaacag cgacgacgag atcgtcgtcg agacccggcg cggggtggtc cgcatcccgc
    12481 gcgatctcgt cgtcgcgacg cgcatcatcc cgcccaagcc gcagcgccgg ggcgtggtgg
    12541 gcggcagcct ggcgccgctc gagctgcatc gtgcggtcgc cgacggctgg ccggccatga
    12601 cgaccgactc cctcggcgac tggctgctcc gcgcgtcgcg cggcttcact ggccgcgcca
    12661 attccgttct ccccgtgggt gatcccggac tcccattgat tccggcactg gatgcggtcg
    12721 aggcgtggta tgccgcccgc caccttcccg cgaatctcac catcgccggg ccggtcgggt
    12781 tcgacccggc cgctgatcct gtcggcgcgg ctgtgctggc gcgcgggtat gtcgtgtcgg
    12841 accccagcct cttcctcacc gcggacgcgg cagaggtcag ccgtgagctc tccgacgctg
    12901 ccacgcaaac cgttgcggca gttggcgatt ccgtgagcgt ggaactcggg gacgagctga
    12961 ctgaggtttg gctcgcggca tacggcggat atcgctccgg agatcgcgag gagatccacg
    13021 cggtcatgac cgggtccccc gcgcaacgct tcgcgacggc acgggctgac ggcaccgtcg
    13081 tcggggtggg gcggctcggc gtgagcggag actgggccgg catcgccgcg atgtgggtcg
    13141 acccggcata ccgccggcgg ggcctcgccc gcgcgatgat gcgcgaactc gccagcgccg
    13201 caccggatct cggcgcttcc agactgcacc tgcaggtctg ggcgcacaat gcgccggccc
    13261 tcgcgctcta tgagcgcatc gggttccgcc gccaccacgc ctatgtcaat gccgtcactt
    13321 ccgggtgagc ctgcccatag gcgcggatgg cctcgagcac cggcgcggcg tcgtggccgg
    13381 cctcggtcag ggtgtaggtc accgagaccg gtggaccggg ctcgacgtgc cggatgacga
    13441 gcccccgggc gcacaactcc ttcagccggg ccgagagcac cgcgtcggtg gcaccgccga
    13501 catagcgcgc gatctccgtg aagcgcgtgt tgccggcgag cagggcttcg atgacggcgc
    13561 ccgcccaggc ccgtccgagg acgcccatga caccctcgat caccgggcag gacgccccgc
    13621 cggagcgctc cggcggagcg agcttgcggg ccgtcaccgg ccggctcccg agaccgactg
    13681 cagcagctcg tcgaagggcg tgaagctgcc gagcagtggg ccggcgtcga tctccgcgtg
    13741 accggaaccc gccggctccg gggcttccgc aaccgtgacg ccaccgacca gggtgcgcaa
    13801 ttcccttgcc gcacggtgga ttcggcgggt caactcgggg gcgcctgggc cgccgaagtc
    13861 ggccgtcgcg gcatacaccc cggtgggcac gacgagcagg ccgaggtagg cggcgagggg
    13921 tcgcatcgcg tgttcgatga cgagcgagtg ccgctcgctg ccaccggtgg cggccaacat
    13981 cgtgggcgtg ccgcgcacgg cgtcgggctc gacgaggtcc gcgaaggact tgaagaggcc
    14041 ggcatacgac ccttggaagg tcggggtgac gagcaccagg gcatcggcgt cacgcacgct
    14101 cgccaacgcc tccgcgagtt cccccgaggg gaagccggtg agcagtgcgt cggtgatggc
    14161 gtgggcgtgc tggcgcagat cgatgtgccg gatctgcgcg tcggccacct cggccgagat
    14221 ggcctccgcg agacggtcgg cgagcatccg ggtcgaactc ggctctcgca gaacggcatt
    14281 gacgacggtg atggtggtca tgcgttcctc cgttcggcgt cggcagacgc ggtcagagcg
    14341 cgctcggcgc gttgtcggtc tcggcggtgg tgccggtcca gcggtcggtg accggggcgg
    14401 catacgactg atcggtctcc ttcgtcgcgg tggcgcgaca ggcggccagc agcgaggcat
    14461 gggttggcgc gtcgggcacg tgcccgggct tcagggcggc aaactccttg cgcaacaccg
    14521 ggatcacctc tccgccgagg atgtcgagct gctcgagcac ggtctgcagc ggcaggccgg
    14581 cgtggtcgat gaggaagagc tgacgctggt agtcaccgac atactcacgg aagccgagcg
    14641 tgcgctcgat gacctgctgc gggctgccca cggtgagcgg ggtctcgcgc atgaaggact
    14701 cgagcgaggg gccaccgcca tacaccggcg cgttgtcgaa gtaggggcgg aactcgcgga
    14761 tcgcgtcctg gctgttggcg cgcatgaaga cctgcccgcc gagcccgacg atcgcctggt
    14821 cggcgctgcc gtggccgtaa tgctcgaagc gccggcgata gagatcgacc atccgctccg
    14881 tgtgctcctt gggccagaag atgtggttgt ggaagaaggc gtcgccatag aacgctgctt
    14941 gctccgcgat ctgcgggctg cggatcgacc cgtgccagac caccggcggg atgccgtcca
    15001 acggccgcgg gaccgaggtg aatccgtaga gcggggtgcg gtacttgccc tcgaaggaga
    15061 cattctcctc acgccacagc ttgtgcagca gggcgtagtt ctcgacggcc aggtcgatgc
    15121 cgtcgcggat gtccttgccg aaccacggat agaccgggcc ggtgttgccg cggccgagca
    15181 tcaggtcgat gcgcccgtcc gcgaggtgct gggcataggc gtagtcctcg gccaggtgca
    15241 ccggatcgtt ggtcgtgatg agcgtcgtcg ccgtcgagag gatgagcttc tcggtcttgg
    15301 cggcgagatg ggcgagcagc accggcgggt tgccgggcgc ggcgaagggc gggttgtggt
    15361 gctccccggt cgcgaagacg tcgagaccga cctcttcggc cttctgcgcg atcgcgaccg
    15421 tcgccttgat gcgctcgtgc tcggtcaggg tggtcccggt caccggatcg atggtcaggt
    15481 caccgacggt gaagatgccg aactgcatga tgcgctcctg ttgtcgtggc actggggtct
    15541 gccactatca acaacatagc cgctcaggta ttccgagtgg tgcagcggcc ctcgtctcag
    15601 ccgtggggcg cgtactcctc gtcgtcgtcg aggaagccgt cggcgcgttc gttgagctcg
    15661 taggaaccct tgccgtcgat gctctcgcgg atgatgtcgg cgtggccggt gtgccgggcg
    15721 acctcggcgg tgatgtgtgc cagcacccag cgcgcctccc agctggccag gtccggcggg
    15781 aaccacggcg cgtcgggcac cggcacggca gcggccaggt cggtgacgga ccggatgttc
    15841 gcgtcggtcg tcgcaacgca ccggtcgaaa tagtcgagag tgtccgcgag gttcgtcgcc
    15901 tcagcgacac actcctcgat cgtctgttcc tcgccgaact cctcggggat caccgggtcc
    15961 cccggcgcgg ccgcggccgc agcgctccag tgcagtgcca cctgcccggc atgccgcagc
    16021 agcccgccga tggtcaggct cgacaccgaa ggcgtcgcgc ggatctgttc gtccgtcagg
    16081 ccgtatgcgg tggaccgcat ctgcgccagc tgctgcagtg cgtaggtgcg cagcacctcc
    16141 agctccccgg atgcgtcggg agtcaggaat cccatcgatg tctcctctgc tcgtcacgga
    16201 cgatcaccgc agcatggcag ccagggcacc ggccgtggcc cacctcgaag ttgacgcttt
    16261 ggcgaactct gagagcgcct cgccgcgccc gccggcccac ggcgccccgg gatggtgcac
    16321 accagctatg agtagcgtgg gcagtccccg tggtcatccc tctgaccagc accgatatcg
    16381 gcggtcacga ggtgtggccc gcctactcat agctggtgtg cacccgcccg cagcctccta
    16441 agcctgccgg gcctgcccgc cgaagtcccg gcgggcgcat tacgacggtt atccacagcc
    16501 ggctccggga tgccgcgaca gatcccccat cacgcgacac ccttcgagca tgacagtcag
    16561 cgagacgctg cgcgaactcg gcggcttcgc cacctatcgc gcgctgcggc aacggcattc
    16621 gcgcaaagag ttggtccagg cgcacgcggc cggcgcgatc gtgcgggagt cccgaggccg
    16681 gtatgccgtc ccggaggtca cctcgcacgc ggccatcgcg caccgttaca ccgcggtcca
    16741 gtcccacctg agcgcagcgc tcgcgcacgg gtggaaggtc aagacggtgc cggatcgtcc
    16801 gtggctgttg ctgaccccgg gacggcgcat acccaagggc ctgagggacg aggctgagta
    16861 cgtctgccgc gcgacactga gcacgcacga acgcaagacc ggcgtgacgg ctccgctgcg
    16921 caccgtgatc gactgcgctc gcgagctgcc tttcgacgag gcgctggcgg tcgccgactc
    16981 ggcgttgcga tcgaagaagg tgcggcggga ggacctgcgc gcggttgctg ctgctgcccg
    17041 cggtcacgat gccgaccgga tccgcacagt ggtggccgct gcggacgggc gggcggccaa
    17101 ccccttcgag tccgtcctgc gggccatcgt cctggagatc gacggactgc acgtcatccc
    17161 gcaggcgcag atcgccgagc cggggctctt cgcggtcgtg gacctggccg accgggcgcg
    17221 caagctggtc atcgaggcgg agggatacga gacccacggc acccagtcgg gtttcctcaa
    17281 ggacgtgcgc cgctattccg aactggcgat gtacggatgg cgggtgttgc gcttcacctg
    17341 gatcgacgtg atgtttcacc cacagtgggt gcgctgggtc atcgagacat ggctggccgt
    17401 ggacgacggc acgcaactgc ctccccaacc ggacgacagc cccggggttt atgaccgcgc
    17461 gagctgatcg gtgcacgcca gctacgagtg acagcagatg cgctcgacag caggcctctg
    17521 acctgcacct tcgtcaaaca atccgacgcg ggcaccggct actcgtagct ggtgtgcacc
    17581 ggtgtcggct actacccggc gttggg
//
LOCUS       DAOPJG010000050        17448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3435_length_17448_cov_7.281953, whole genome shotgun sequence.
ACCESSION   DAOPJG010000050 DAOPJG010000000
VERSION     DAOPJG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3435_length_17448_cov_7.281953"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKU75_06620"
     CDS             complement(<1..338)
                     /locus_tag="PKU75_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPF80587.1"
                     /translation="MADIESTGPGLGHREISFTRTDQGRYAARNARGGELAFGSGDDT
                     DFTPVELLLVAIAGCSAVDVDLITGKRADPTRFEVTATGTKVRDEQGNRLQDITISFT
                     VLFTGTTTGTS"
     gene            378..992
                     /locus_tag="PKU75_06625"
     CDS             378..992
                     /locus_tag="PKU75_06625"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF80588.1"
                     /translation="MSPEKPPKGKLVLLRHGETEWSKSGQHTGLTDLPLTAHGEELAH
                     AAGSLLGGYDFGLILASPLQRAARTAELAGLHAETDPDLVEWDYGSAEGRTTKEIRAE
                     LGYDWSVFTHGVQPGATPGEQVEEVAARMSRIIAKAMPVLAERDVALIGHGHALRILT
                     TVWLRQEVRFAAQLLLDAGSVSVLQYDKEFPSVRTWNRLPALPE"
     gene            complement(996..2480)
                     /locus_tag="PKU75_06630"
     CDS             complement(996..2480)
                     /locus_tag="PKU75_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HPF80589.1"
                     /translation="MGAEVDAKSYSREERQRYREKVRQNLDVFERMLSNSKFEFEKPM
                     TGMELELNLVDGDFQPRFANLDVLAAIADADFTTELAQYNIEMNVRPRPLPGDAALEL
                     EEDLRRALNNAEQKAHSAEARIVPIGILPTIMPEHFEGDWITDNNRYWALNDSIFYAR
                     GEDIYLDIEGPTGERVSTYSDTIAPESACTSMQLHLQVAPQDFAAYWNAAQAIAGPQL
                     ALGANSPFFFGKRLHAETRIELFKQATDTRSIELKNQGVRPRVFFGERWITSIFDLFE
                     ENVRYFPALLAECSDEDPLAQLDAGIAPNLPELRLHNGTVYRWNRPIYDVVGGRPHLR
                     VENRVLPAGPSMIDVLANAAFYYGVVRTLADDDRPVWSRMSFAAAEENFRRCSRDGID
                     ARIYWPGFGEVPADELVLRHLLPMAHEGLAVWGVSQAVRDRYLSVIEGRCTTGINGAV
                     WQSETVAHLEARGMERPQALREMLSRYIEHVHGNEPVHTWPRAE"
     gene            2622..3797
                     /locus_tag="PKU75_06635"
     CDS             2622..3797
                     /locus_tag="PKU75_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPF80590.1"
                     /translation="MRLVHTSDWHLGRSLGGVGLLGAQALVLDDLVATVRREQVDAVL
                     VSGDVYDKALPNPDAVTLLSETFERLVDAGAQVVVTSGNHDSAIRLGFASRLLQRAGL
                     HVRTSLAEVGTPVLVAGVAIYPLPYLEPALTADALQASRRSHAAVLEAAMTRVRADLA
                     SRGGPSVVMGHAFVSGGLSCESERDISIGGVAAVPVETFAGVSYAALGHLHGRQQAGP
                     NVHYSGSPVAMSFGEAGHVKGYAVVEIEGMAAPRVELVRAPVERALAVLRGRLEELLT
                     QPQYAAAEASFVAVTLTDPVRPLGAMDRIRRRFPHAIKLEWQPEGGFGQERRTYAARV
                     ADRSDLDICCDFLAHVRGGVAAGPGERRLLREAVEASRVGRAVREEEGVVTPASGVA"
     gene            3794..6982
                     /locus_tag="PKU75_06640"
     CDS             3794..6982
                     /locus_tag="PKU75_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HPF80591.1"
                     /translation="MRLHHLSVEAFGPFVARQEIDFDDVSGAGVFLVHGPTGAGKTSL
                     LDAICFALFSDVPGDRQARGLRSELAEPDCPPRVRLEFTAAGRRLRVERTAEFTRPKR
                     RGTGQVRVQSTVTLSEFAGSQWRPVSTRIDEAAEVLQDLLGMGLNQFRRVALLPQGDF
                     AAFLHTTPEARRALLERLFDVERFTTLESWFAERRRTLTQQVAAAQQALHHQIATLTD
                     VLATAPPAALAPLSDADELAFAAVPVGEWAGLVPRLEVALSAWAGDEFGAFQRAETQE
                     QAAGARRDEAERIAGLRARARVASDALTRLREDATRIEVLRQRAADGLAAAGLRAELA
                     AVASAERIAAEAVSRLQAARQSAAGAGLDPDAAAGLVPTLESGARSLADLGRNVEQAR
                     ALAAEIVDLAEAVARAGETAVQLQSEAATAATQQAAARTRCAALVAVAGSAPLWASRR
                     READAMQRLQHALATSSVRLTAAEGAQSDATAALVLAQETLIAGQRARLGDLAAELAV
                     TLTDGDPCPVCGSVEHPAAQTDRAQWDPATIAAAEAEVERCRDHLEKCRLDVARAVEA
                     RAGQLREMHERLAALAAGEPPSHLAALLTDLLARESLADTETAATLARLAREVGAQHD
                     AATTAATALQAAEAEVEALDEQRQAIQERLAAARDRAAAAGARQDAAQVRLDETLARA
                     RHQISAHSAECVCGATDPELDSDNDDEGVASDVVAMLAAWHREATERHTAVERLVAGI
                     VAADDRAREAEAAYAAADAALRVALRGSVFATREKLRQALMSTDDLRAAQHAVAEHDR
                     LTAIHEATLADEQVRAALAVTDPPDPALAASAAEQARAACLEAARRHTTAQRTTEQAT
                     GIAREIVFTATQSAPLHAQLDEVGELADLLAGTSANNALRMRLSAFVLAARLERVVEL
                     ANERLAMMDAGRYRLLYDDARAARGARSGLGLLVHDEWSGTTRAPGSLSGGESFMASL
                     ALALGLADAIRESSGGQEVQTLFVDEGFGSLDEESLENVMTVLDGLRDGGRTVGIVSH
                     VGDLRDRIPVRIEVRKSPHGSSVVLTAADSAA"
     gene            complement(6996..9842)
                     /gene="gcvP"
                     /locus_tag="PKU75_06645"
     CDS             complement(6996..9842)
                     /gene="gcvP"
                     /locus_tag="PKU75_06645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPF80592.1"
                     /translation="MTTPLPEFAARHIGPRDADIDAMLQAIGQDSLDALASSAIPHTI
                     RMAEALDIEPATSEQAVLDQLRAYADDNRVLTSMIGQGYYGTHTPGVIQRNVLENPAW
                     YTAYTPYQPEISQGRLEALVNFQTMITDLTGLDIAGSSLLDEATAAAEAMTLMHRATR
                     AKADAVLLVDAHVFPQTLAVVRTRAEAIGIPLVVTDLTEMTTGEALRAAAGEKDVFGV
                     LVQYPDAQGAIRSYAALAVAAHEAGALVTAAADLLALTLLKPPGEWGADIAVGSAQRF
                     GVPMMFGGPHAGFMSVRKGLERTMPGRLVGVSVDSAGYPAYRLALQTREQHIRREKAT
                     SNICTAQVLLAVMASMYAVYHGPAGLSAIARRVHERAQDIAAGLSAAGIEPLGTHYFD
                     TLTFSVPGRADEFVAAAAEAGINLRRGADDEVGLSVDETTTPDQVARVLGAIVSGMVA
                     GEPTEAAIPADLQRTSDFLTHPVFNSYHSETAMLRYLRSLADKDFALDRGMIPLGSCT
                     MKLNATTEMAAITWPEFATLHPFAPDDQSAGYQALIAELEDWLCRITGYDAVSVQPNA
                     GSQGELAGLLAIRAFHRANGQEHRTVCLIPQSAHGTNAASAVMAGMKVVVVATGPGGD
                     IDMVDLKNKIADHGDNLAAIMVTYPSTHGVYEDTIAELCAMIHDAGGQVYVDGANLNA
                     LVGLAQPGTFGSDVSHLNLHKTFCIPHGGGGPGVGPIGVRAHLAPFLPASPQQGRTGS
                     AVSAAPYGSAGILPISWAYIRLMGGAGLTRATQVAVLNANYIAARLREHYPVLYSGKN
                     GLVAHECILDVRPLTKESGVTVDDIAKRLIDYGFHAPTMSFPVAGTLMVEPTESEDKG
                     ELDRFCDAMIAIRGEIQQVIDGQVAIGDSVLRHAPHTTQSLAGEWDHPYDRVTACFPQ
                     GLHATKYWPPVGRIDGAYGDRHLICSCPPPEAFE"
     gene            complement(9976..10524)
                     /locus_tag="PKU75_06650"
     CDS             complement(9976..10524)
                     /locus_tag="PKU75_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPF80593.1"
                     /translation="MRPTPRPTQDALFDDFGTPFGDVGYRGVTACKAAGITYRQLDYW
                     ARTGLVEPSIRGAAGSGSQRLYGFRDILVLKVVKRLLDTGVSLHQIRLAIEHLRERGV
                     EDLAQVTLLSDGASVFECTSPTEMFDLLQGGQGVFGIALGKVWQEVEGSLAHLPGESP
                     DEATPAEHPGDELRARRAARVG"
     gene            complement(10698..11174)
                     /locus_tag="PKU75_06655"
     CDS             complement(10698..11174)
                     /locus_tag="PKU75_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPF80594.1"
                     /translation="MRHLDVLGVRVEMPTNTPIILLREREGARHLPIWIGAAEATAIA
                     YAQQGVVPPRPLTHDLLVNTITALGDSLGSVRIDRIDEGVYYATLVLGSGIEIDSRAS
                     DAIALAIRAGAEVYAPEDLLDSAGILLPEETDDEVEKFKEFLDHVSPEDFETRPDS"
     gene            complement(11187..11873)
                     /locus_tag="PKU75_06660"
     CDS             complement(11187..11873)
                     /locus_tag="PKU75_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPF80595.1"
                     /translation="MPARGQRIGAVLAQLTPDYPDLTISKLRFLEAEGLVEPERAPSG
                     YRLYAPADIERVRFILTAQRDRFWPLKVIKDNLDAMDRGLTVDAGTSAVPTPPQVTAD
                     PAVPRAEDLVADEQPAPRLQATELAAATGAGRTLIDELVSFGLLRPDKQGYFPGESAR
                     IVAAAHDLGGFGIEARHLRVFRTAAEREVGLVDYAAAGAGRPDPAAIAAACLALHTAL
                     VKDELSRREA"
     gene            complement(11877..12353)
                     /locus_tag="PKU75_06665"
     CDS             complement(11877..12353)
                     /locus_tag="PKU75_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPF80596.1"
                     /translation="MSDQPAAPTPDSGEPSGEVFRFDPSTQRLPGVGGSEAPDWTDYE
                     PALSREDLGLIEALRPGTALLIVQRGPNTGARFLLDDAEVTSGRGPDSDIFLDDVTVS
                     RKHAVFSFEDGRFVVRDVGSLNGTYVNRERVDEAELHDRDEVQIGKFRLVFHQAKA"
     gene            complement(12390..12779)
                     /gene="gcvH"
                     /locus_tag="PKU75_06670"
     CDS             complement(12390..12779)
                     /gene="gcvH"
                     /locus_tag="PKU75_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPF80597.1"
                     /translation="MSDFDFPDDLRYTSDHEWVRVGGDGNVRIGITAYAQDALGDVVY
                     VSAPAVGSPVAAGDACGEVESTKSVSDLYSPLTGTVTAVNDALDGAPELINSDPYGEG
                     WIYEMTVEDPAVLETLMDAGAYRDNLA"
     gene            complement(12839..13609)
                     /locus_tag="PKU75_06675"
     CDS             complement(12839..13609)
                     /locus_tag="PKU75_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPF80598.1"
                     /translation="MATRTRRPATDTRTAWRRLGRIAAPRMTRSNLFVLALAVLLGFA
                     ISAQVRQTRTQGLENLRQEELVGILDTVTQESVRLGNELRDLERIRDRLASGADSEAA
                     AVQVAQERADTLAILAGTKAATGPGITMTITDPDGQISSTILLDALEELRDAGAEVIQ
                     IGPVRVVASTYFTDVDGGISVSGTNIAPPYIFRAIGDAPTMAAAMEIPGGVGESVRGK
                     GGEVTIAARDEVSITATYEPKTPQFARPAPTPSSSASS"
     gene            complement(13609..13941)
                     /locus_tag="PKU75_06680"
     CDS             complement(13609..13941)
                     /locus_tag="PKU75_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HPF80599.1"
                     /translation="MIPALGLLAGILIGVVLQPDVPIWLQPYLPIAVIAALDAVFGGV
                     RAVLDGIFDDKVFVISFLSNVIVAALIVYLGDQLGVGAQLSTGVVVVLGVRIFSNVAS
                     IRRHLFHA"
     gene            complement(13938..14792)
                     /locus_tag="PKU75_06685"
     CDS             complement(13938..14792)
                     /locus_tag="PKU75_06685"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPF80600.1"
                     /translation="MTAPAPTPARRPDASMTLLTEMLERPLDPGYAAASARRAAAGGP
                     RSTPVGSTLVAVTAILIGLLFAVSATTVRRGEASAGTVRKDLISQIESRRAVVDERSA
                     AIRDRQTEITRLESNALGATENGLRQELQALAALTGNVAVEGPGLLVTLNDKPTDDAT
                     PTAGDDQGKVLSRDLFIVVNSLWQSGAEAIAINGQRLTVRSPIRFAGEAILVNFRPLS
                     PPYVISAIGDPAALPAAFAETVGGAYLKSLTDNFGIVTSVEGADHISLPGATRVTVDK
                     AVVQGGTK"
     gene            complement(14800..15402)
                     /locus_tag="PKU75_06690"
     CDS             complement(14800..15402)
                     /locus_tag="PKU75_06690"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPF80601.1"
                     /translation="MTTTPVEPERSDRVWTIPNLLSMLRLVGVPIFLWAILTKHDTLA
                     LVVLMLSGISDYLDGKIARKFNLISNLGALLDPIADRLYILSTLLGLAWREIIPWWLV
                     GVLIAREVFLTLLLLYARTKGLREMPVHFVGKAATFNLLYAFPCLLLADGDSTLAQIA
                     RPIGWGFAWWGIVLYWVAGLMYAVQLRRYVRAATLHPTAT"
     gene            15450..16592
                     /locus_tag="PKU75_06695"
     CDS             15450..16592
                     /locus_tag="PKU75_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF80602.1"
                     /translation="MSEPILNLGLDALRERISLKWNTYPADVIPAWVAEMDVVPAAPI
                     REALAAMMERGDTGYPTASGYVGAFGDFAARRWGWQIDRDRARLSADVMMGIFVVLLK
                     TTSEGDPVVINDPVYYPFRTFPELGNRPIVWAAMGEDGRLDLAALEAAFAEATARGGR
                     AAYILSNPHNPTGVVHTVAELTAVAGLADRFGVTVISDEVHAPVVYAGSPRFVSYLQV
                     PGLERGFAVHSAAKAFSLAAFKAGVVLGAPGEDVTLKEIMTGPNASLSGVVAHTAAFT
                     HGDAWLDQVLGELDENRRLVQRLLAEQAPSVTTALPEATYLMWLDCRAAGLPRDPAAH
                     FLEVAKVGLNSGPSFGSRGEGFARLNIATSPAILTEIVGRIAASLP"
     gene            complement(16596..17087)
                     /locus_tag="PKU75_06700"
     CDS             complement(16596..17087)
                     /locus_tag="PKU75_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80603.1"
                     /translation="MSAPPVLPRPAPPRAVVRRLGLTAGALVALYGLYAGLATLGTVP
                     PLPWSKTVATWTLPEEQDPTPATQELTVMVSRIECNGGRTGTLRGAKVNEHDDEVVVT
                     TYVAKQRPGAYTCLGNDLKPLTVPLKTPLGERKLVDGACTDATRQTALCLDDGVRWPR
                     AAD"
BASE COUNT         2537 a         5800 c         6426 g         2685 t
ORIGIN      
        1 gacgttccgg tcgtggtgcc ggtgaacaag acggtgaagc tgatcgtgat gtcttgcagg
       61 cggtttccct gctcgtcgcg caccttggtg ccggtcgcgg tgacctcgaa gcgcgtgggg
      121 tcggcgcgtt tgccggtgat caggtcgacg tccacggcgc tgcatccggc gatggcgacg
      181 agcagcaact ccacgggagt gaagtcggtg tcgtctcccg acccgaacgc cagctcgcca
      241 cccctggcgt tgcgggcggc ataccgtccc tggtcggtgc gggtgaaact gatctcccgg
      301 tgcccgaggc cgggacccgt cgattcgatg tcagccatgg gcggcacgct accgaacagg
      361 ccttagggtc gacttccatg agtcccgaaa aaccgcccaa gggcaagctg gtcctgctcc
      421 ggcacggcga gaccgagtgg tcgaagtccg gccagcacac cggcctgacc gatctgccgc
      481 tgacggcgca cggcgaggaa ctcgcccacg ccgccggcag cctcctcggc ggctacgact
      541 tcggtctcat cctcgcctct cccctgcagc gggcggcccg caccgccgaa ctcgccggcc
      601 tgcacgccga gaccgacccc gacctcgtgg agtgggacta cggctcggcc gagggccgca
      661 ccaccaagga gatccgggcc gagctgggct acgactggtc ggtgttcacc cacggcgtgc
      721 aacccggggc gacgccgggc gagcaggtcg aggaggtcgc ggcccggatg tcgcggatca
      781 tcgccaaggc gatgccggtg ctcgcggagc gggacgtcgc actcatcggt cacggccacg
      841 cgctgcggat cctgacgacc gtatggctgc gtcaggaggt ccgtttcgcg gcgcagctgc
      901 tgctcgatgc gggctcggtc agcgtgctcc agtacgacaa ggagttcccg tccgtgcgca
      961 cgtggaaccg ccttccggcg ttgcccgagt agggatcact cggcccgggg ccaggtgtgg
     1021 accggttcgt tgccgtgcac gtgctcgata tagcgcgaga gcatctcgcg cagcgcctgg
     1081 ggccgctcca tcccccgggc ctccaggtgc gcgaccgtct cggactgcca tacggcgccg
     1141 ttgatcccgg tggtgcaccg tccctcgatc accgagagat agcggtcgcg cacggcctgc
     1201 gagacacccc atacggccaa tccctcgtgg gccatcggga gcaggtgacg cagcaccaac
     1261 tcgtccgccg gcacctcgcc gaaacccggc cagtagatgc gcgcgtcgat gccgtcgcga
     1321 gagcagcggc ggaaattctc ttctgccgca gcaaaactca tccgcgacca tacgggtcgg
     1381 tcatcgtcgg cgagcgtgcg caccactccg tagtagaagg cagcgttggc gagcacgtcg
     1441 atcatcgacg gcccggcggg cagcacacgg ttctcgacac gcaggtgcgg cctgccgccg
     1501 acgacgtcgt agatcgggcg gttccagcga tagacggtgc cattgtgcag gcgcagctcg
     1561 ggcaggttcg gcgcgatgcc ggcgtcgagc tgcgcgagcg gatcctcgtc gctgcactcg
     1621 gccagcaggg ccgggaagta tcggacgttc tcctcgaaca ggtcgaagat cgaggtgatc
     1681 cagcgctcgc cgaagaagac ccgcgggcgc acgccctggt tcttcaactc gatcgagcgg
     1741 gtgtcggtgg cttgcttgaa gagctcgatg cgggtctcgg cgtgcaggcg cttgccgaag
     1801 aagaagggcg aattggcccc gagcgcgagc tgcgggcccg cgatcgcctg ggcggcattc
     1861 cagtaggcgg cgaaatcctg gggcgcgacc tgcaggtgca attgcatcga ggtgcacgcc
     1921 gattccgggg cgatggtgtc gctgtaggtc gacacccgct cccccgtcgg gccttcgatg
     1981 tcgaggtaga tgtcctcgcc gcgggcatag aagatggagt cgttgagggc ccaatagcgg
     2041 ttgttgtccg tgatccagtc gccctcgaag tgctcgggca tgatcgtcgg gaggatcccg
     2101 atcgggacga tgcgcgcctc ggcgctgtgg gccttctgct cggcattgtt cagcgcccgg
     2161 cgcaggtcct cctcgagttc gagggccgcg tcgcctggca gcggccgcgg ccggacgttc
     2221 atctcgatgt tgtactgcgc cagttcggtc gtgaaatcgg cgtcggcgat ggcggccagc
     2281 acatcgaggt tggcgaaccg gggctggaag tcgccgtcga cgaggttgag ttcgagttcc
     2341 atgccggtca tcggcttctc gaactcgaac ttgctgttgg agagcatgcg ctcgaagacg
     2401 tcgaggttct ggcgcacctt ctcgcggtag cgctgtcgct cttcgcggct gtagctcttg
     2461 gcatcgacct ccgcacccat gcgctcaagc gaaccacacc cggggcccca gcggcggctt
     2521 gtcttgacag ctggctggaa tcgcgccccc acggcgggtc gcgggcggcg caccggggca
     2581 aatccgcagg gttgtcggtg ggctctccta gcctgcagga catgcggctt gtgcacacct
     2641 ccgactggca cctggggcgt tccctcggtg gcgtcgggct gctcggtgcc caggcgctcg
     2701 tgctcgacga tctggtggcg acggtgcgcc gcgagcaggt cgatgctgtg ctggtcagcg
     2761 gtgatgtcta tgacaaggcg ctccccaatc ccgatgcggt cacgctgttg tcggagacgt
     2821 tcgagcggct cgtcgacgcc ggtgcccagg tcgtggtcac cagcggcaat cacgactcgg
     2881 cgatccgcct gggtttcgcc tcccggctgc tccagcgtgc cggcctgcac gtgcggacct
     2941 cgctcgcgga ggtgggcacg ccggtgctcg tcgccggggt cgcgatctac cccctcccct
     3001 atctcgagcc ggcgctgacc gccgacgccc tgcaggcgtc aaggcgctcg cacgcggccg
     3061 tcctcgaggc ggcgatgacg cgggtgcgcg cggatcttgc gtcccggggc gggccgtcgg
     3121 tggtgatggg gcacgccttc gtctccggtg gcctctcctg tgagtccgaa cgcgatatct
     3181 ccatcggcgg ggtcgccgcc gtgccggtcg agaccttcgc cggggtgtcc tatgccgcct
     3241 tgggtcacct ccacggtcgc cagcaggcgg gtccgaacgt ccactacagc gggtcgccgg
     3301 tggcgatgtc gttcggtgag gccgggcacg tcaagggcta tgccgtcgtc gagatcgagg
     3361 gtatggccgc gccgcgggtc gagttggtcc gggcgccggt cgagcgggcg ctggcggtgc
     3421 tgcggggccg gctggaggag ttgctgacgc agccgcagta tgccgccgcc gaggcttcgt
     3481 tcgtcgccgt caccctcacc gatccggtgc gtccgctggg cgccatggac cggatccggc
     3541 gccggttccc gcacgcgatc aagctcgaat ggcaacccga gggcggcttc gggcaggagc
     3601 gccgcaccta tgccgcccgg gtcgccgacc gcagcgacct cgacatctgc tgcgacttcc
     3661 tcgcccatgt gcgcggtggt gtcgctgcgg ggcccgggga gcgccggctg ttgcgcgagg
     3721 cggtcgaggc gagtcgggtc ggtcgcgcgg tgcgggagga ggagggtgtg gtcacgccgg
     3781 catccggggt ggcatgagac tgcaccatct cagcgtcgag gccttcgggc cgttcgtcgc
     3841 gcgccaggag atcgacttcg acgatgtgtc cggggccggg gtgttcctcg tgcacggccc
     3901 gaccggggcc ggcaagacca gcctgctgga cgcgatctgc ttcgccctct tctccgacgt
     3961 cccgggcgac cggcaggccc gcggcctgcg cagcgaactc gccgaacccg actgcccgcc
     4021 gcgggtgcgc ctggagttca ccgcggccgg ccggcggctg cgggtcgagc gcaccgcgga
     4081 gttcacccgg cccaagcggc gcggcaccgg acaggtgcgc gtgcagtcga ccgtgacgtt
     4141 gagcgagttc gcgggcagcc agtggcggcc ggtgagcacg cgcatcgacg aggcggccga
     4201 ggtgctgcag gatctgctcg ggatgggcct gaaccagttc cggcgcgttg cgctgctccc
     4261 ccagggcgac ttcgctgcct tcctgcacac cacacccgag gcgcgccgcg ccctgctcga
     4321 gcggctcttc gacgtcgagc gcttcacgac actggagtcc tggttcgccg agcggcggcg
     4381 gacgctgacg caacaggtcg ccgcggcgca gcaggcgctg caccaccaga tcgcgacgtt
     4441 gaccgacgtc ttggccactg cgcccccggc ggcgctggct cccctgtccg atgccgacga
     4501 actcgcgttc gcggcggtgc ccgtcgggga atgggcgggc ctcgtgccgc ggctcgaggt
     4561 ggccctgtcg gcctgggcgg gtgacgaatt cggcgctttc cagcgagccg agacccagga
     4621 gcaggccgcg ggtgcccgtc gggacgaggc cgagcgcatc gccgggttgc gggctcgggc
     4681 gcgcgtggcg tctgacgctc tcacgcgctt gcgcgaggat gccacccgga tcgaggtcct
     4741 ccggcagcgg gcggccgacg ggctggccgc ggcggggttg cgggccgagc tggcggcggt
     4801 ggcctcggcc gaacggatcg cggccgaggc cgtttcacgc ctgcaggcgg cgcggcagtc
     4861 ggccgcgggg gccgggctcg acccggatgc cgcggccggc ctggtcccca cgctggagag
     4921 cggcgcccgg agcctcgcgg acctgggccg caacgtggag caggcacgcg cgctggccgc
     4981 cgagatcgtc gatctcgccg aagcggtggc gcgtgccgga gagacggcgg ttcagctgca
     5041 gtcggaggcg gcgacggcag cgacccagca ggcggcggca cgaacgcgtt gtgccgcact
     5101 cgtcgccgtc gcgggctcgg ccccgctgtg ggcatcacgg cgccgtgagg ccgacgcgat
     5161 gcagcgcctt cagcacgcgc tggccacctc gtcggtccgg ctcacggcag ctgagggtgc
     5221 gcagtcggat gccacggccg cattggtcct cgcccaggag accctgatcg ccggccagcg
     5281 agcccgactc ggcgacctcg ccgccgagct ggccgtgacc ttgaccgacg gagacccctg
     5341 ccccgtatgc ggttcggtgg agcaccccgc tgcgcagacc gatcgcgcgc agtgggatcc
     5401 ggcgacgatc gcggccgccg aagcggaggt cgagcggtgt cgtgatcatc tcgagaagtg
     5461 ccgtctggac gtcgcgcgcg cggtcgaggc tcgcgccggg cagctgcgtg agatgcacga
     5521 gcgcctggcc gctctggccg ccggcgaacc accgtcccat ctcgccgcgc tcctcaccga
     5581 tctgcttgcg cgcgagtcct tggctgacac cgaaaccgca gcgactctgg cgcgactggc
     5641 ccgcgaggtc ggtgcacagc atgacgcggc cacgacggcc gcgaccgcgc tgcaggcggc
     5701 tgaggcggag gtcgaagcac tcgacgagca gcggcaagcc atccaggagc gcctcgcagc
     5761 ggcccgggac agggcggccg cggccggcgc acggcaggac gccgcgcagg tccggctcga
     5821 cgagaccctc gccagggcac gtcaccagat cagtgcccac tcggccgaat gtgtctgcgg
     5881 tgcaaccgat cccgagctcg acagtgacaa cgacgacgag ggagtcgctt ctgacgtcgt
     5941 cgcgatgctg gcggcctggc accgtgaggc aaccgagcgg cataccgccg tcgagcggct
     6001 cgtggccggc atcgtcgccg ccgacgaccg cgcacgcgag gccgaagccg cttacgcggc
     6061 cgccgacgcc gcgctccggg tcgccttgcg cggcagtgtc tttgccacgc gtgagaagct
     6121 ccggcaagcc ctcatgagca ccgacgacct gcgcgcggcg cagcacgccg tcgccgagca
     6181 cgaccggctc accgcgatcc acgaggcaac acttgccgac gagcaggtgc gcgcggccct
     6241 ggccgtgacc gacccacccg accccgcgct ggctgcgtcc gcggcggagc aggcgagagc
     6301 ggcctgtctg gaggcggcgc ggcggcatac caccgcccag cggacgaccg agcaggccac
     6361 gggcatcgcc cgcgaaatcg tcttcacagc aacccaatcg gctcccctgc acgcgcaact
     6421 ggacgaggtg ggcgagctgg ccgacctgct cgccggcacc agcgccaaca acgcgctgcg
     6481 gatgcggctg tccgcgttcg tgctcgccgc gaggctcgaa cgggttgtcg aactggccaa
     6541 cgagcgacta gcgatgatgg atgccggccg ctaccgcctc ctctacgacg acgcccgcgc
     6601 tgcccgcggg gcccgcagcg gcctgggcct actcgtccac gacgagtggt cgggcacgac
     6661 gcgcgcccca gggagcctct cggggggcga gtcgttcatg gcctcgctcg ccctcgctct
     6721 cgggctcgcc gacgccatcc gcgaaagcag cggcggccag gaagtgcaga ccctgttcgt
     6781 cgacgagggc ttcggcagcc tcgacgagga gagcctcgag aatgtcatga ccgtcctgga
     6841 cggcctgcgc gacggcggac gcacggtcgg catcgtcagc catgtgggtg acctgcgtga
     6901 ccgcatcccg gtgcggatcg aagtgcgcaa gtcaccccac gggtccagcg tggtgctgac
     6961 cgcggcggac tcggccgcct gaggcgcggc cggctctact cgaacgcctc cggcggcggg
     7021 cagctgcaga tcagatggcg gtcgccgtat gccccgtcga tccggccgac cgggggccag
     7081 tacttcgtcg cgtgcaggcc ctgcgggaaa caggcggtga cccggtcgta ggggtggtcc
     7141 cactcccccg cgagcgactg ggtcgtgtgc ggcgcatgcc gcagcacgct gtccccgatc
     7201 gcgacctgac cgtcgatgac ctgctggatc tcgccgcgga tcgcgatcat cgcgtcgcag
     7261 aagcggtcca gctcgccctt gtcctccgac tcggtcggct cgaccatgag cgtgccggcc
     7321 accgggaagg acatcgtcgg ggcgtggaag ccgtagtcga tgaggcgctt ggcgatgtcg
     7381 tcgacggtga caccgctctc cttggtcagc gggcgcacgt cgaggatgca ctcgtgcgcg
     7441 acgaggccgt tcttgcccga atagagcacc gggtagtgct cgcgcagccg ggcggcgatg
     7501 tagttggcgt tcaggaccgc gacctgggtg gcgcgcgtca ggccggcgcc gcccatgagc
     7561 cggatatagg cccacgagat cggcaggatc cccgccgagc cgtagggcgc cgcggagacc
     7621 gcggacccgg tgcggccctg ttgcgggctg gccggcagga agggggcgag gtgtgcacgc
     7681 acgccgatcg ggccgacacc ggggccgccg ccgccgtgcg ggatgcagaa ggtcttgtgc
     7741 aggttcaggt gcgagacgtc ggagccgaac gtgccgggct gcgccagccc gacgagcgcg
     7801 ttgaggttgg cgccgtcgac gtagacctga ccgccagcgt catggatcat cgcgcacaac
     7861 tccgcgatcg tgtcctcgta gacgccatgg gtggaggggt aggtcaccat gatcgcagcg
     7921 aggttgtcgc cgtggtcggc gatcttgttc ttcaggtcga ccatgtcgat atcgccgccg
     7981 ggaccggtcg cgacgacgac gaccttcatc cccgccatga cggcgctcgc ggcattggtg
     8041 ccgtgcgcgc tctgtgggat caggcagacg gtgcggtgtt cctgcccgtt cgcgcggtgg
     8101 aaggcgcgga tggcgagcaa cccagccagc tccccctggg agccggcgtt gggctggacc
     8161 gaaaccgcgt catacccggt gatccggcac agccagtcct cgagctcggc gatgagcgcc
     8221 tgatagcccg ccgactggtc gtccggcgcg aaggggtgga gggtcgcgaa ctcgggccag
     8281 gtgatcgccg ccatctcggt cgtggcgttc agcttcatcg tgcacgagcc gagcgggatc
     8341 atgccgcggt cgagggcgaa gtccttgtcg gcgagcgagc gcaggtagcg cagcatcgcc
     8401 gtctcggagt gatagctgtt gaagaccggg tgcgtgagga agtccgaggt gcgttgcaga
     8461 tcggcgggga tcgccgcctc cgtgggctcg cccgcgacca tcccggacac gatcgcgccc
     8521 aacacccgag cgacctggtc gggggtcgtc gtctcgtcga cgctgaggcc gacctcgtcg
     8581 tcggctccgc gccgcaggtt gatcccggcc tccgctgcgg ccgcgacgaa ctcgtcggcc
     8641 cggccgggca cgctgaaggt cagggtgtcg aagtagtggg tgccgagcgg ttcgattccc
     8701 gcggccgaca ggcccgccgc gatgtcctgt gcgcgctcgt gaacccgccg agcgatggcg
     8761 ctcaacccgg ccggtccgtg gtagacggca tacatcgagg ccatcacggc gagcagcacc
     8821 tgcgcggtgc agatattgga ggtcgccttc tcgcggcgga tgtgctgctc gcgggtctgc
     8881 agggcgaggc ggtatgccgg gtagccggcc gagtcgaccg acacgccgac gaggcggccg
     8941 ggcatcgtgc gctcgagtcc cttgcgcacg ctcatgaagc cggcgtgcgg gccgccgaac
     9001 atcatcggca ccccgaaccg ctgggccgac ccgaccgcga tgtcggcgcc ccactccccc
     9061 ggcggcttca gcagcgtgag cgcgaggagg tcggcggcgg cggtgaccag cgctccggcc
     9121 tcgtgcgcgg cgacggccag cgcggcatac gaccggatcg cgccctgcgc gtcgggatac
     9181 tgcaccagca ccccgaagac gtccttctcg ccggcggcgg cgcgcagcgc ctccccggtg
     9241 gtcatctcgg tgaggtcggt gacgaccagc ggtatgccga tcgcctcggc ccgcgtgcgc
     9301 accaccgcca gcgtctgcgg gaacacgtgg gcgtcgacga gcaggacggc gtcggccttg
     9361 gcccgggtag cgcgatgcat cagcgtcatg gcttcggccg cggcagtcgc ctcgtcgagg
     9421 agggaggagc cggcgatgtc gagaccggtc aggtccgtga tcatcgtctg gaagttgacg
     9481 agggcctcca ggcggccctg gctgatctcc ggctggtagg gcgtgtaggc ggtgtaccag
     9541 gccgggttct ccagaacatt gcgctggatc accccggggg tgtgcgtgcc gtagtagccc
     9601 tgcccgatca tgctcgtcag cactcggttg tcgtccgcgt acgcgcgcag ctggtccagg
     9661 acggcctgct cgctggtggc cggctcgatg tcgagcgctt cggccatccg gatggtgtgc
     9721 gggatcgccg aagacgccag ggcatcgagg ctgtcctggc cgatcgcctg caacatggcg
     9781 tcgatatcgg cgtcgcgcgg gccgatgtgc cgggcagcga attcgggcag gggtgtcgtc
     9841 accgggactc ctcgtggggt gcttttggcc acttgcgagc ctatcaacgg cacctgccct
     9901 cggacccctc acgcctggta cgacgatcgg cgagccgtga cccggcgatt gtcggtgccg
     9961 cggctgggaa gggcgtcagc cgacgcgggc ggcgcggcgg gcgcgcagtt cgtcgccggg
    10021 gtgctcggcg ggggtggcct cgtcgggtga ctcgccgggc aggtgggcga gcgagccctc
    10081 gacctcctgc cagaccttgc ccagggcgat cccgaagacc ccctgaccgc cttggagcag
    10141 gtcgaacatc tcggtcggcg aggtgcactc gaagacggag gcgccatcgc tcaggagggt
    10201 gacctgggcc aggtcctcca caccccgctc gcgtaggtgc tcgatggcca ggcggatctg
    10261 gtgcagggag acgccggtgt ccaggaggcg cttgacgacc ttgaggacga ggatgtcgcg
    10321 gaagccgtag aggcgctggc tgccggagcc ggccgcaccg cgaatggacg gctcgacgag
    10381 cccggtccgg gcccagtagt cgagctggcg ataggtgata cccgcggcct tgcaggccgt
    10441 caccccgcgg tagccaacgt ccccgaaggg cgtcccgaag tcgtcgaaca gcgcgtcctg
    10501 cgtgggtcgc ggcgtcggcc gcatcccatc cccgctctcg ctcaccgaat cctccgtgtg
    10561 gacttgactg gtatctggat ttgacggtac ggcgggcggg ggcggtcgtc aacgaccgac
    10621 gccataacac tttcggcgtg tcgcgcctga cccactagtc acatcagtca caggcatcat
    10681 taaaggtcga cttgaggtca agagtcgggt cgggtctcga agtcctccgg cgaaacgtgg
    10741 tcgaggaact ccttgaactt ctcgacctcg tcgtcggtct cctccggcag caggatcccg
    10801 gcgctgtcga gcagatcctc gggtgcgtag acctcggcac ccgcccggat cgcgagagcg
    10861 atggcgtcgg aggcgcggga gtcgatctcg atgccgctgc ccaacaccag ggtcgcgtaa
    10921 tagacgccct cgtcgatgcg gtcgatgcgc acgctgccga ggctgtcgcc caaggccgta
    10981 atggtgttga cgagcaggtc gtgcgtcaac ggccggggcg gcacgacgcc ctgctgggcg
    11041 taggcgatgg cggtggcctc ggccgcgccg atccaaatgg gcaggtggcg ggcaccctcg
    11101 cgctcgcgca acaggatgat cggggtgttc gtgggcattt cgacccgcac ccccaggacg
    11161 tccaagtgcc tcaccctgac aaactactac gcctcgcgcc ggctcagttc gtccttgacg
    11221 agggcggtat gcagcgccag gcaggccgcg gcgatcgcgg ccgggtccgg acggcccgcc
    11281 ccggcggcgg cgtagtcgac caaccccacc tcgcgttcgg cggctgtgcg gaagacccgc
    11341 agatggcggg cttcgatccc gaagccgccc aggtcgtggg ccgcggccac gatccgcgcg
    11401 ctctccccgg ggaagtagcc ctgcttgtcg gggcgcagca gcccgaagga gaccagttcg
    11461 tcgatgagcg tgcggccggc ccccgtggca gcggccagtt cggtggcctg cagccgcgga
    11521 gccggctgct cgtcggccac caggtcctcc gcccgtggca ccgccggatc ggccgtgacc
    11581 tgaggcggcg tgggcacggc ggaggtgccg gcgtcgaccg tcaggccgcg gtccatcgcg
    11641 tcgaggttgt ccttgatcac cttcagcggc cagaatcggt cgcgctgagc ggtgaggatg
    11701 aaccggaccc gctcgatatc cgccggggcg tagaggcggt atccggatgg ggctcgctcg
    11761 ggctcgacga ggccttcggc ctcgaggaag cgcagcttgg agatcgtcag gtcgggatag
    11821 tccggcgtga gctgcgcgag cacggccccg atgcgctggc cgcgggcagg cactggtcag
    11881 gccttggcct ggtggaacac caggcggaac ttgccgatct gcacctcgtc gcgatcgtgc
    11941 aactcggcct cgtcgacgcg ttcgcggttg acataggtgc cattgaggga acccacgtcg
    12001 cgcaccacga accggccgtc ctcgaaggag aacacggcgt gcttgcggga gaccgtcacg
    12061 tcgtcgagga agatgtcgct gtccgggccg cggccggagg tcacctccgc gtcgtcgagg
    12121 aggaaacgag caccggtgtt ggggccgcgc tggacgatca gcaacgcagt gccggggcgc
    12181 agagcctcga tcaggccgag gtcctcgcgg ctgagggccg gctcgtagtc ggtccagtcg
    12241 ggggcctcgg acccgccgac gccgggcagg cgctgggtcg aggggtcgaa acggaacacc
    12301 tcgccggacg gctcgccaga gtcgggcgtc ggggccgctg gctgatcgct cattcgatgt
    12361 gctccttcac gggtgggcgg gtgcccaggt caggcgaggt tatcgcggta ggcaccggca
    12421 tccatcagcg tttcgaggac cgcggggtcc tcgacggtca tctcgtagat ccagccctcc
    12481 ccgtaggggt cgctgttgat cagctcgggg gcgccgtcga gggcgtcgtt gacggcggtg
    12541 acggtgccgg tcaacggaga atagaggtcg ctgaccgatt tggtcgactc gacctcgccg
    12601 caggcgtcgc ctgcggccac cggcgacccc acggcggggg cactgacata tacgacatcg
    12661 ccgagcgcgt cctgggcgta tgccgtgatg ccgatccgca cattgccgtc gccgccgacg
    12721 cgcacccact cgtgatccga ggtgtagcgc agatcgtcgg ggaagtcgaa atcagacatc
    12781 gcagtctccg ttcgtggatt ggttgggcgc aacactagtc cgcgcgggcg tggaagcgtc
    12841 aggacgacgc gctggagctc ggcgtgggtg cggggcgggc gaactggggg gtcttcggct
    12901 cgtaggtcgc ggtgatgctc acctcgtccc gggcggcgat cgtcacctcg ccgcccttgc
    12961 cacggaccga ctcccccacg ccgcccggga tctccatcgc ggcagccatc gtcggggcgt
    13021 cgccgatggc ccggaagatg tagggcgggg cgatattggt gccggagacg ctgatcccgc
    13081 cgtcgacgtc ggtgaagtag gtcgaggcga ccacacgcac cgggccgatc tggatgacct
    13141 ccgcgccggc gtcgcgcaac tcctcgagcg cgtcgaggag gatcgtggag ctgatctggc
    13201 cgtcggggtc ggtgatggtc atcgtgatgc ccggtccggt cgccgccttg gtgcccgcca
    13261 ggatggcgag ggtgtcggcg cgttcctgcg ccacctggac ggcggcagcc tcgctgtcgg
    13321 cgccgctggc gagccggtcg cggatgcgtt ccagatcgcg cagttcgttg ccgagccgca
    13381 cactctcctg tgtcacggtg tcgagaatgc cgacgagctc ctcctggcgg aggttctcca
    13441 gcccctgcgt gcgggtctgt cgcacttgcg cgctgatcgc gaacccgagg aggaccgcca
    13501 gcgccagcac gaacaggttg ctgcgggtca tgcgaggggc ggcgatccgc ccgaggcggc
    13561 gccacgcggt gcgcgtgtcc gtggcggggc ggcgggtgcg ggtggccatc acgcgtggaa
    13621 gaggtggcgg cggatcgagg cgacattgga gaagatccgc accccgagga cgacgacgac
    13681 accggtcgag agttgcgcac cgacaccgag ctggtcgccg agatagacga tcaacgccgc
    13741 cacgatcaca ttggagagga aggagatgac gaagaccttg tcgtcgaaga tgccgtcgag
    13801 gacggcccgg accccgccga agaccgcgtc gagcgcagcg atgacggcga tcggcagata
    13861 gggctgcagc cagatcggca catcgggttg caggacgacg ccgatgagga tgccggcgag
    13921 tagccccagg gcgggaatca cttggtgcct ccttggacga cggccttgtc gacggtcacg
    13981 cgggtggcgc cggggaggct gatgtggtca gccccttcga cggatgtgac gatgccgaaa
    14041 ttgtcggtca acgatttgag ataggccccg cccaccgtct cggcgaacgc cgcgggcagg
    14101 gccgccgggt cgccgatcgc cgagatgacg tagggcgggg acaggggccg gaagttgacg
    14161 aggatcgcct cgccggcgaa gcggatcggc gagcggaccg tcaggcgctg gccgttgatg
    14221 gcgatcgcct cggccccgga ttgccacagc gagttgacga cgatgaacag gtcccgggag
    14281 agcaccttgc cctggtcgtc gcccgctgtg ggcgtcgcgt cgtcggtggg cttgtcgttg
    14341 agggtgacga gcaggccggg tccttcgacg gcgacgttcc cggtgagggc cgcaagggcc
    14401 tgcagttcct gccgcagccc gttttcggtg gcgcccaagg cgtttgactc gagccgggtt
    14461 atctccgtct gccgatcacg gatggcggcc gagcgttcgt cgaccacggc gcggcgactc
    14521 tcgatctggc tgatcaggtc cttgcgcacg gtcccggcgc tcgcttcccc gcggcgcacc
    14581 gtcgtggccg agacggcgaa gagcaacccg atcaggatgg cggtgacggc cacgagggtg
    14641 gacccgacgg gagtggaacg aggaccaccg gcggcggcac gacgagccga cgccgcggca
    14701 tacccggggt cgaggggacg ctcgagcatc tcggtcagca gcgtcatcga cgcgtcgggg
    14761 cgacgcgcgg gagtgggtgc aggcgctgtc atacgaccct cacgtggcgg tggggtgcaa
    14821 ggtcgccgcg cggacatacc ggcgcaactg cacggcatac atcagcccgg cgacccaata
    14881 aaggacgatg ccccaccacg cgaagcccca cccgatcggg cgggcgatct gcgccagggt
    14941 ggagtcgccg tcggccagca gcaggcacgg gaaggcgtag aggaggttga aggtcgcggc
    15001 cttgccgacg aagtggaccg gcatctcgcg caggcccttg gtgcgggcat acagcagcag
    15061 gagggtgagg aaaacctcgc gggcgatcag gacgccgacc agccaccacg gaatgatctc
    15121 gcgccaggca agcccgagga gggtggagag gatgtagagg cggtcggcga tggggtcgag
    15181 gagggcgccg aggttggaga tcaggttgaa cttgcgcgcg atcttgccgt cgaggtagtc
    15241 gctgatgccg gaaagcatca acaccacgag ggcgagcgtg tcgtgcttgg tcaggatggc
    15301 ccacaggaag atgggcacac cgaccagtcg cagcatcgac aacaggttcg gaatcgtcca
    15361 cacgcggtcc gagcgctcgg gctcaaccgg tgtcgtcgtc acgtgggaag cctaaacggt
    15421 tccgctggtg agccgttaca ttccctcgta tgagcgagcc catcctcaac ctcggcctgg
    15481 atgcactgcg cgagcggatc agcctgaagt ggaacaccta tccggcggac gtcatcccgg
    15541 cgtgggtggc tgagatggac gtcgtgccgg cggcgccgat ccgcgaggcg ctcgcggcga
    15601 tgatggagcg cggcgacacc ggttatccga cggcttcggg gtatgtcggt gccttcggtg
    15661 atttcgccgc gcgccggtgg gggtggcaga tcgatcgcga ccgggcgcgg ctgtcggcgg
    15721 acgtgatgat ggggatcttc gtcgtcctgc tcaagacgac gtccgagggt gacccggtgg
    15781 tcatcaacga cccggtgtac taccccttcc ggaccttccc tgaactgggg aaccggccga
    15841 tcgtgtgggc ggcgatgggc gaggacgggc ggctggacct ggcggcgctg gaggcggcct
    15901 tcgccgaggc gacggcgcgg ggcgggcggg cggcatacat cttgtccaac ccgcacaacc
    15961 cgaccggggt ggtgcacacg gtggcggaac tcaccgcggt cgccgggctg gcggaccggt
    16021 tcggggtgac ggtgatctcg gacgaggtgc acgcgccggt ggtgtatgcc ggctctcccc
    16081 gtttcgtgtc ctatctgcag gtgccggggc tggagcgcgg gttcgccgtg cactcggcgg
    16141 ccaaggcgtt ctcgctggcg gcgttcaagg cgggcgtcgt gctgggggcg ccgggtgagg
    16201 acgtcacgtt gaaggagatc atgaccgggc cgaatgccag cctgtccggg gtggtggctc
    16261 acacggcggc gttcacccac ggggatgcgt ggctcgatca ggtgctcggc gagctggacg
    16321 agaaccggcg gctcgtgcag cggctgctgg cggagcaggc gccgtcggtc acgaccgccc
    16381 tgccggaggc gacctatctg atgtggctgg actgccgggc ggccggcttg cctcgcgacc
    16441 cggcggcgca cttcctcgag gtcgccaagg tggggctcaa ctccggcccg tcgttcggct
    16501 cccgtggcga gggcttcgcg cggctgaaca ttgcgacctc gccggccatc ctgaccgaga
    16561 tcgtcgggcg gatcgcggcc tcgctgccct gacccttagt cggcggcgcg gggccagcgc
    16621 acgccgtcgt cgaggcagag agcggtctgg cgggtggcgt cggtgcaggc accgtccacc
    16681 agctttcgct cgcccagggg cgtcttgagc ggcacggtca gtggtttgag gtcgttgccg
    16741 aggcaggtgt aggcgccggg ccgctgcttc gcgacatacg tcgtgacgac gacctcgtcg
    16801 tcgtgttcgt tcaccttggc tccgcggagc gtgccggtgc gcccgccgtt gcactcgatg
    16861 cgcgacacca tcaccgtgag ttcctgggtc gccggcgtgg ggtcctgctc ctcgggcagc
    16921 gtccacgtgg caacggtctt gctccacggc aacggcggca ccgtgcccaa ggtggccagg
    16981 ccggcataca gcccgtacag cgccaccagg gccccggctg tcagccccag ccggcgcacc
    17041 acggcgcgag gcggggcggg gcgtggcagg acgggtggtg cagacacgcc agaagtgtcc
    17101 acctaccagc gagttcagag caaactactg gggcgggagt ggctcgtgtt gcgatctgga
    17161 tggcggggtc acaccgccgg tagagcgtgg ccctcgaaca ccgtcgttag acccgccgac
    17221 gacgtgggaa atccgcgtgg tcgggctgac aggatttgaa cctgcgaccc cttgaccccc
    17281 aggcgtccag atacctcccc tcgaaccgaa atccgtgcgg ttccgggcct ccgatcatgc
    17341 agaccacgtc acgagatgca tggatttcat gattctggtc cccttttggt cccctgtcgg
    17401 cggccacgag aaactgcccg gagacggcca cgaagctgcc cgctggcg
//
LOCUS       DAOPJG010000051        17323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3467_length_17323_cov_7.994788, whole genome shotgun sequence.
ACCESSION   DAOPJG010000051 DAOPJG010000000
VERSION     DAOPJG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17323
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3467_length_17323_cov_7.994788"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..896)
                     /locus_tag="PKU75_06705"
     CDS             complement(144..896)
                     /locus_tag="PKU75_06705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPF80604.1"
                     /translation="MPRGPLPAWARVRAVLLDVDDTLIDTRAAMHQAGTVASGVVWPA
                     ADRSRIALAGERFRSDPDGFFGAYTRGESTFDAMRRARIGALATWLGQELGEGHAGAW
                     LAAYEPAFEAALRAHTDAEVALTRLAAHGIPVGFLTNSGADYTARKLALTGLGGLVDV
                     ICTRDTLGFGKPDARAFHEACRRLGTAPAETLYVGDELLSDALGAADAGMPAGWLVRD
                     GAPDPGEADVVAGRGIPVLANLIQVADGLGCP"
     gene            complement(906..2423)
                     /gene="gltX"
                     /locus_tag="PKU75_06710"
     CDS             complement(906..2423)
                     /gene="gltX"
                     /locus_tag="PKU75_06710"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPF80605.1"
                     /translation="MSTKDLGPVRLRVAPSPTGDPHVGTAYMSLFNLAFARQQGGQFI
                     LRIEDTDRARFRHDSEKQVFDTLHWLGLTWDEGPDIGGPVGPYRQSERLATYQPYVEK
                     LLREGKAYHCWCSPERLQQMRAEQQKNKQPTGYDRLCYGKTEAQRRELPGFSETPVVR
                     MLIPDDVPLEFDDLIRGKVSAPRPDDQVILKADGFPTYHLAVVVDDHEMKVTHVVRGE
                     EWISSTPKHLLLYRWLGLETPKFAHMPLLRNEDKSKISKRKNPAARLTWFVEQGYLPE
                     ALVNFLALLAYPPAQDAEGNDVEKFTFAEFSRDFAWEKVNPVGPIFDLKKLEWLNGVY
                     IRDLELGDFTSRLLPYLEREGVLSGNPGLGELGRLQDVAALIQTRISLLKDAVPLVRP
                     FFVGDDMLEIAEDARAGLGEDAKDVLDKAIDVLGDIDDHLVSLVAPSAWNAENIEAKL
                     REAIVDGLGKKARVAFGPIRVAVSGAKISPPLFESMAILGKTSTLARLVALRDSL"
     assembly_gap    2479..2578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2714..2962
                     /locus_tag="PKU75_06715"
     CDS             2714..2962
                     /locus_tag="PKU75_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPF80606.1"
                     /translation="MTRIVVNTHQAKSRLSELIREAEGGAEVIVARGGRPVARIVPWS
                     DPRPHRSPGRWAGRIRYSGDLVDSDPEVLADFEASAQS"
     gene            2959..3342
                     /locus_tag="PKU75_06720"
     CDS             2959..3342
                     /locus_tag="PKU75_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPF80607.1"
                     /translation="MSRLLLDSHVLVWWLGDSRDLGPTTRTAITEAESVFISAVTPWE
                     LGIKAALGRLDLPGDLLGILAEEGFEALTISLEHGARAAALPWHHRDPFDRMLIAQAV
                     AEDLVLVTADAQLTAYDVRLLPARD"
     gene            complement(3378..4148)
                     /locus_tag="PKU75_06725"
     CDS             complement(3378..4148)
                     /locus_tag="PKU75_06725"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPF80608.1"
                     /translation="MRIVRFTDGQDPAYGVLEEDGQVVAEIVGDPLYQGVQFTGNRVA
                     LNQVRLLAPVIPRSKIIGIGRNYADHAKELGNEVPTSPMAFFIPNTAVVGPDDPVVIP
                     DFAKEIHYEGELAVVIGRIAKDVPRERWQEVVFGYTCANDVSARDLQRGDGQWARAKG
                     MDTFCPLGPAIETSLDPSDLRVITRRDGEVVQDGTTADLIFDIPTLIEFLSAAFTLLP
                     GDVILTGTPAGVGPVDHGQRVEVEIEGIGTLANPFVRR"
     gene            4209..5108
                     /locus_tag="PKU75_06730"
     CDS             4209..5108
                     /locus_tag="PKU75_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HPF80609.1"
                     /translation="MSRTPMPRGEWIAHEAAHAARADAATAGHLARRASGAKHPVEDF
                     LFEYYNFTPGKLRRWHPGPGVTLADAADTPRAHWPGYVSDDDGSVRLDVPAFVARRGS
                     TIDFTRQLLRATASRPGFTGCFGLHEWAMVYRLAPEQIRHAAYPLRLSPKDTDTVVEA
                     HTIRCSHFDAFRFFTQDALARNTIQPTRDTQVANEQPGCLHAGMDLYKWAHKLTPLTP
                     GDILLDAFDLARDLRTLDMQASPYDLADLGYPPLRIETPEGKAEYAARQRAFAERSNA
                     LRLRLLAVLDDVAEATSKVHDTP"
     gene            complement(5120..5884)
                     /locus_tag="PKU75_06735"
     CDS             complement(5120..5884)
                     /locus_tag="PKU75_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80610.1"
                     /translation="MTVTTAGEGGVAEKQGQVWRLQVGPRVHEVISREATVTKREVLW
                     LVDGELVATKTTSEEKVVLTPEEEEGAHLGAIRVIHTAFNAPRRAIWFEGQGASAQAL
                     TGLGGVDMEPEAGSPVAVREERMARRPGLYAARHVAGGVMKVVGPVIVAWLIARSAGL
                     LPGLDLNLPRIPLPDIDLPDLPWPDINLPSIPWPDWNAPGWLVWIVGHAKYVVPILIG
                     IAVARSEIRRRQTTAQRREKLRSRDLDQESKERDTP"
     gene            6091..6736
                     /locus_tag="PKU75_06740"
                     /pseudo
     CDS             6091..6736
                     /locus_tag="PKU75_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(6747..8345)
                     /gene="cimA"
                     /locus_tag="PKU75_06745"
     CDS             complement(6747..8345)
                     /gene="cimA"
                     /locus_tag="PKU75_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPF80611.1"
                     /translation="MNELHVYDTTLRDGAQQEGLNLSVTDKLAIAAHLDELGVGFIEG
                     GWPGANPKDTEFFRRAQTELQLKNATLTAFGATRRAGGRAESDPLVRALLDSGAPVVT
                     LVAKSHVGHVERALRTTLEENLEMIRDTVTFLRGEGRRVFLDAEHFFDGYTTNRSYAV
                     EAVRAAAEAGAEVIALCDTNGGMLPNQVADVVGDVVGATGARIGIHCHNDTGCAVANS
                     MAALDAGATHVQGTINGYGERTGNANLVTIVANLQIKQGRELVEPHRLAEATRISHAI
                     SEITNVAPYGRQPYTGSSAFAHKAGLHASAIKVDPDLYQHTDPKSVGNDMRMLVSDMA
                     GRASIELKSRELGLALDGADEQDAAKLAQVLATVKDKEMRGYTFDAADASFELLLRRE
                     LNGGLPDFFDVESWRVITDSRGDDDALSEATVKVSAGGERVVKTGEGNGPVNALDQAL
                     REALSTAYPEISHLELVDYKVRILDTEHGTDAITRVLIETSDGSTTWSTIGVAANILE
                     ASWDALLESVTYGLIRAGVEPRRG"
     gene            complement(8532..8975)
                     /locus_tag="PKU75_06750"
     CDS             complement(8532..8975)
                     /locus_tag="PKU75_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HPF80612.1"
                     /translation="MGTTLGSLVTDLACVGVFALIGRAAHDEGLSLGGWFDTAWPFLA
                     GLGVGWLGVLLMRDRPAPWLVRPGVVIWLATVIVGMLLRVVIDAGTATSFIVVALVFN
                     GLLLVGWRVFIQLSQFFSRWADRMGTERARRDRAAQRRAAVRGRR"
     gene            complement(9132..9938)
                     /locus_tag="PKU75_06755"
     CDS             complement(9132..9938)
                     /locus_tag="PKU75_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HPF80613.1"
                     /translation="MIATDLDGTLLRSDGSVSARTAAALAAVAEAGILTVLVTARPPR
                     WLHDLSHVVGAHGIAICGNGAFVYDVVSRAVVEVHTFAPAAVAEVVADLRERLPHITF
                     AAETAVGCFVEESWPNPHEEDADARTMIPLIESLVASQDAGHRIGKLLALAPGDPTDA
                     FLAAVAEVAGEAAVLAYSGAFGLAELNPPGVTKAAGLERWCAERGVAAADVWAFGDMP
                     NDLPMLTWAGRSFAMGNAHPDVLAATTDRCRTNDEDGVATVLEELVEIGC"
     gene            complement(10010..11110)
                     /locus_tag="PKU75_06760"
     CDS             complement(10010..11110)
                     /locus_tag="PKU75_06760"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPF80614.1"
                     /translation="MTALSFTHTPNANPASDADRAAILADPGFGKHFTDHMVTATWTT
                     DAGWHDGAVTAYGPISLMPSAAVLHYAQEIFEGLKAYRHEDGSVWGFRPHANAQRMAR
                     SATRLALPPLPEEDFLASLRAIVEADKAWVPGPGESGEKSLYLRPFMFASEAFLGVRP
                     AAEVTYSVIASPAGAYFAGGLKPVTLWISEHYARAGAGGTGAAKCGGNYASSLAGQLE
                     GAEHGCDQAVFLDSSTHSYIEELGGMNLFFVTKDHRLVTPELTGTILEGVTRSSILDL
                     SKELGLDPEERRIEIEEWKDGVRSGDIVEIFACGTAAVVTPVGELRWEGGQAPSTAGE
                     AGGEVTNAIRSRLLDVQYGRVEDTHGWLTQLA"
     gene            complement(11307..12380)
                     /locus_tag="PKU75_06765"
     CDS             complement(11307..12380)
                     /locus_tag="PKU75_06765"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPF80615.1"
                     /translation="MSDTAMSPGSRTIDLAVIGGDGIGPEVVAEGLKVLEAVGASEGL
                     TFARTEYDLGAKRWHATGETLPDSVVEELRGQDAILLGAIGDPTVPSGVLERGVLLPL
                     RFALDHYVNLRPAKLFPGVASPLDVSAVAPNGIDFVVVREGTEGPYVGNGGTVREGTP
                     HEIATEVSVNTRFGVERVVRDAFARAQARPRKHLTLVHKHNVLVNAGHLWRRTVEEVG
                     AEFPDVETAYQHVDAATIFMATDPGRFDVIVTDNLFGDIITDIAAAIAGGIGLAASGN
                     INPTGAAPSMFEPVHGSAPDIAGQAKADPTATVLSVAMLLEHLGYADAAARVDAAVAA
                     DLAERGTAARSTTEVGDAIAARL"
     gene            complement(12447..14033)
                     /gene="serA"
                     /locus_tag="PKU75_06770"
     CDS             complement(12447..14033)
                     /gene="serA"
                     /locus_tag="PKU75_06770"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPF80616.1"
                     /translation="MTKPVVLIAEELSPATIDALGPDFEVRTCDGANRSELLAALPEA
                     EAVLIRSATKMDAEAIAAAKSLKVIARAGVGLDNVDVPAATKAGVMVVNAPTSNITSA
                     AELAVGLMLAAARNIAPANQALKGGAWKRSMYGGVELLDKKVGIVGFGRIGQLVAERL
                     KGFGMEILAYDPYVSAQKAGALGARLVTLDELLAESDFISIHLPKTAETLGLIGTEAL
                     AKAKPGVIIVNAARGGIIDEAALADAIASGHIGGAGLDVFAAEPTTESPLFDHEKVVV
                     TPHLGASTEEAQEKAGISVAKSVRLALAGDIVPDAVNVAGGVIAEEVRPGIDLVEKLG
                     RIFTAVAGSVPVQLDIDVRGEITEFDVSIWKLSALKGLFTDITNEPVTYVNAPLMAQE
                     RGCEARLITHPVAEEFRNVTTLRGTLADGSIVEVAGTLTGPKMVQKIIGVNGFDLEVP
                     ISTHMAFLSYVDRPGVIGAFGRLLGDAGVNIAGMQLSRKEEGGAALVVLTLDSAIPAP
                     VLSAIAAEVDATLAEVVTLD"
     gene            complement(14181..14729)
                     /locus_tag="PKU75_06775"
     CDS             complement(14181..14729)
                     /locus_tag="PKU75_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPF80617.1"
                     /translation="MDELVDGWPFWAVYLLFFCGAMVRAHVTYAAGRGLRAGATRAAH
                     PTAADTWLARLSRHIDRPGVQRAERLMSRYGAPLVTLSFLTVGIQTVLNAAAGALRMD
                     LRRYTPAAVVGSLLWAGVYTTAGFAALDVVRGTLPWWWLLIALGLVTLVVLVTRLVRG
                     RVESQTATTDDTHLGDDDASAP"
     gene            14770..15540
                     /locus_tag="PKU75_06780"
     CDS             14770..15540
                     /locus_tag="PKU75_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80618.1"
                     /translation="MRVVVTGGLGNLGRAVVSALQDGGHEAVIASRRTGVDLRTGAGL
                     AECLRDADAAVHTADSLRPWHWKTVTLGGTRTLAETAAGLPAPPHLVYISIVGVDRNP
                     YPYYRAKLAAEQVLADAPVPATVVRATQFHSLAAVMAGMRLGPVAVGVRGLSIQPVDI
                     TWVGRRLAEIAVSSRPDGFTRARELAGPDRFEAAEITDLVAAHLGTSAPRRLEMPPLG
                     ATLKAFAAGAVLPRDDAEIGGERFTEWLARQGSRLPRR"
     gene            complement(15560..17107)
                     /locus_tag="PKU75_06785"
     CDS             complement(15560..17107)
                     /locus_tag="PKU75_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPF80619.1"
                     /translation="MRALHPDHERVVTALTTPDLAAVVDLVVWVAPDPDGTPVAHAAN
                     HLGRVRLEPGGRHTVLAGRDPIVSEDPLAFLPYAAELAHPSPPVSTHNAYPLAAERLL
                     SFFADPDRSPDIAIVHTPGHYFPDEGGHVGEHGSLDVIQSRAPLIVSGARVEARGLVD
                     EWARLIDVGPTLAYLAGVPESDLRDAKGDPLDGRVLRRYLNEGPIGPVVGILWDGGHC
                     GDLLHLAESGDLPGVARLIARGTALRGGAIAQFPSITLTNHTSILTGVGPGRHGVLGN
                     VYVDRATGERVVPNDETTWHRSSEWLHDSVRTVFEMVADHIPVPDVAADADDAALAAA
                     RFLRPRTASVDEAIDRGADYSTMQIIRASGSSRGAGGLGDQLPDPHTSPFVHEPEHLE
                     DSYFRWGVQVDDLGLQQMRQLFAGPDHLPALTWWANVVTDAGHHGGGPRSSMARDALR
                     QADARLVAFLDHLDGLGVTDQVTFILTADHGFEGSDPAITGSWAPALAALGIPLRDEG
                     PGLIYLG"
     gene            complement(17235..>17323)
                     /locus_tag="PKU75_06790"
     CDS             complement(17235..>17323)
                     /locus_tag="PKU75_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPF80620.1"
                     /translation="WGGQYFVDGVVVARKRPRGRPRKDSYAA"
BASE COUNT         2609 a         6040 c         5927 g         2647 t
ORIGIN      
        1 agaatgagtg aaccagaatc actagtgttg ccaattacac tataccccaa ggggtatccc
       61 cgccccttcg tgccgaggca cgcgcgagcg gtgaaccgag cggcaatact accggcatcc
      121 gcgcgcgctc caaaatcgcc ggatcatggg caaccgagcc cgtcggcgac ctggatcagg
      181 ttcgccagca ccggtatgcc gcgccccgcc accacgtccg cctcgcccgg gtcgggtgcg
      241 ccgtcgcgca cgagccagcc ggccggcata cccgcgtcgg cggcgccgag cgcgtcggag
      301 agcagttcgt caccgacata cagcgtctcg gcgggcgcgg tgcccagccg ccggcaggcc
      361 tcgtggaacg cccgcgcgtc cggcttgccg aagccgagag tgtcgcgggt gcagatcacg
      421 tcgacgagcc cgccgaggcc ggtgagtgcg agcttgcgtg cggtgtagtc ggcgccggag
      481 ttggtgagga accccacggg tatgccgtgc gccgcgagcc gcgtcaacgc cacctcggcg
      541 tcggtgtgcg ccctcagggc cgcctcgaag gccggttcgt atgccgcgag ccacgccccc
      601 gcatggccct cgccgagctc ttgccccagc caggtcgcca gggcgccgat ccgggcgcgc
      661 cgcatcgcgt cgaaggtcga ctccccgcgg gtgtaagcgc cgaagaagcc gtcgggatca
      721 gagcggaaac gctcgcccgc caacgcgatt cgcgaccgat cggcggccgg ccagaccacc
      781 ccgctcgcga ccgtgcccgc ctggtgcatc gccgcccggg tatcgatcag cgtgtcgtcg
      841 acgtcgagca gcaccgcacg cacccgcgcc caggcgggca gaggtccgcg cggcatcctg
      901 cgggatcaga gcgagtcgcg cagggcgacg aggcgggcca gggtcgaggt cttgccgagg
      961 atcgccatcg actcgaacag cggcggcgag atcttcgcgc ccgagacggc gacgcggatc
     1021 ggcccgaagg cgacgcgcgc cttcttgccc aacccgtcga cgatcgcctc acgcagcttg
     1081 gcctcgatgt tctcggcatt ccacgcgctg ggggcaacga gcgagacgag atggtcgtcg
     1141 atgtcgccga gcacgtcgat ggccttgtcg agcacgtctt tggcgtcctc gcccagtccg
     1201 gcgcgggcgt cctcggcgat ctcgagcatg tcgtcgccga cgaagaacgg ccgcacgaga
     1261 gggacagcgt ccttgagcag ggagatgcgc gtctggatca gcgcggccac gtcctgcagc
     1321 cggccgagct cccccaggcc gggattgccg gacaagacgc cctcgcgctc gagatagggc
     1381 aacaggcgcg aggtgaagtc gcccagctcg aggtcgcgga tgtagacacc gttgagccac
     1441 tcgagcttct tgaggtcgaa gatcggcccc accgggttga ccttctccca cgcgaagtcc
     1501 cgcgagaact ccgcgaacgt gaacttctcg acgtcattgc cctcggcgtc ctgagccggc
     1561 ggataggcaa gcagcgcaag gaaattcacg agcgcctcag gcaggtagcc ctgctcgacg
     1621 aaccacgtca gccgcgccgc ggggttcttg cgcttggaga tcttggactt gtcctcgttg
     1681 cgcagcagcg gcatgtgcgc gaacttcggg gtctccagcc cgagccagcg atagagcagc
     1741 agatgcttgg gcgtggagga gatccactcc tcgccgcgga cgacgtgcgt caccttcatc
     1801 tcgtggtcgt ccacgacgac cgcgaggtgg taggtcggga acccgtcggc cttgaggatc
     1861 acctggtcgt cgggccgggg cgcgctgacc ttcccccgga tcaggtcgtc gaactccagc
     1921 ggcacgtcgt cggggatgag catccgcacc acgggcgtct cgctgaaacc gggcagttcg
     1981 cgccgctgcg cctcggtctt gccgtagcag agccggtcgt atccggtggg ctgcttgttc
     2041 ttctgctgct ccgcacgcat ctgctgcagc cgctcgggcg agcaccagca gtggtaggcc
     2101 ttgccctccc tgagcagctt ctcgacatac ggctggtatg tcgcgaggcg ctcggactgc
     2161 cggtagggcc cgaccggtcc gccgatgtcg gggccttcgt cccaggtcag ccccagccag
     2221 tgcagggtgt cgaagacctg cttctcgctg tcgtggcgga agcgggcgcg gtcggtgtcc
     2281 tcgatccgca ggatgaactg gccgccctgc tggcgcgcga aggcgaggtt gaacagggac
     2341 atataggcgg tgccgacgtg cgggtccccc gtgggagagg gggcgacgcg cagccgcacc
     2401 ggaccgaggt ctttggtgct catagtgtcc tcgattctat gtatgccgcc gctatgtatg
     2461 ccgcgctatg gatgccgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     2581 tgtcctcgat tctatgtatg ccgccgctat gtatgccgcg ctatggatgc cgctgcgtac
     2641 gcagcgtccg gctatgccgc atcctcgcac ccacatcctc gcacccaaga agggacttag
     2701 ctaagctagg accatgacca ggatcgtcgt gaacacccat caggcgaagt cccggctctc
     2761 ggagctgatc cgcgaggccg agggcggtgc cgaggtgatc gttgcgcgcg gcggccgacc
     2821 ggtggcccgc atcgtgccct ggtccgaccc acgacctcac cggtcgccgg gtcgctgggc
     2881 cggccggatc cgctacagcg gcgacctcgt cgacagcgat ccggaggtac tcgccgattt
     2941 cgaggcatcg gcccagtcgt gagccggctc ctcctcgaca gccacgtcct ggtctggtgg
     3001 ctcggtgatt ctcgcgacct ggggccgacc acccggaccg cgatcaccga ggccgagtcc
     3061 gtcttcatct ccgcggtcac gccgtgggaa ctcgggatca aggcagccct cggcaggctc
     3121 gacctgcccg gcgacctgct cggcatcctc gccgaggagg ggttcgaagc gctcaccatc
     3181 tccctcgagc acggggcgcg agcagcggcg ctgccttggc atcaccgcga tcccttcgat
     3241 cggatgctca tcgcccaggc cgtcgccgag gatctggtcc tcgtcactgc ggacgcgcag
     3301 ctgaccgcat atgacgttcg gctgctgccg gcgcgagact gatcggctcg gcctccggac
     3361 gcccgtgctt tccgttgtca gcggcgcacg aaggggttgg ccaaggtgcc gatgccctcg
     3421 atctccacct cgacccgctg gccgtgatcg accggcccga cgccggcggg ggtgccggtg
     3481 aggatgacat cgcccggcag cagggtgaat gccgcgctga ggaactcgat gagcgtcggg
     3541 atgtcgaaga tcaggtcggc cgtcgtgccg tcctggacga cctcgccatc gcggcgggtg
     3601 atcacgcgca ggtcggaggg gtcgaggctg gtctcgatcg ccgggccgag cgggcagaag
     3661 gtgtccatgc ccttggcccg cgcccactgc ccgtcgccgc gctgcaggtc gcgcgccgag
     3721 acgtcattgg cgcacgtgta gccgaagacg acctcctgcc agcgctccct cggcacatcc
     3781 ttggcgatgc ggccgatgac gacggccagt tcgccctcgt agtggatctc tttggcgaaa
     3841 tcggggatga cgaccgggtc gtccgggccg acgacggcgg tgttggggat gaagaaggcc
     3901 atcggggagg tggggacctc gttgcccagc tccttggcgt ggtcggcgta gttgcgcccg
     3961 atgccgatga tcttgctgcg cggtatgacc ggcgccagta gccgcacctg gttcagcgcg
     4021 acccggttgc ccgtgaactg caccccctgg tagagcgggt cgccgacgat ctcggcgacg
     4081 acctgcccgt cttcctcgag gacgccgtat gccgggtcct gcccgtccgt gaatcgcacg
     4141 atccgcatgg cctccaccct agggccgcat gccgctcgcc ctcacctggt tagggtgacc
     4201 cggtgcggat gagccggacc ccgatgcccc ggggggagtg gatcgcccac gaggctgccc
     4261 acgcggcccg ggccgacgcc gcgacggccg gtcacctcgc gcgccgggcg agcggcgcca
     4321 agcaccccgt cgaggacttc ctcttcgagt actacaactt cacccccggc aagctgcgcc
     4381 gctggcaccc cgggcccggc gtcacgctgg ccgacgcggc cgacaccccg cgcgcgcact
     4441 ggcctggcta tgtcagcgat gacgacggca gcgtccgtct cgacgtcccc gccttcgtcg
     4501 cccgccgcgg gagcaccatc gacttcaccc ggcaactgtt gcgcgccacc gcttcccggc
     4561 cgggcttcac gggctgcttc gggttgcacg agtgggcgat ggtctatcgc cttgcgccgg
     4621 aacagatccg gcacgcggca tacccactgc gattgtctcc caaggacacc gacactgtcg
     4681 tggaagctca caccatccgg tgcagccact tcgatgcctt caggttcttc acccaggatg
     4741 ccttggcgcg caacacgatc cagcccacgc gggacacgca ggtcgccaac gagcagcccg
     4801 gttgcctgca cgccggcatg gatctctaca agtgggcgca caagctcacg ccactgacac
     4861 ccggcgacat cctgctggac gccttcgacc tggcccgcga tctgcgcacc ctcgacatgc
     4921 aggcctcccc ctacgacctc gccgatctcg gctatccccc gctgcgcatc gagacccccg
     4981 agggcaaggc ggagtatgcc gcccgccagc gcgccttcgc cgaacgctcc aacgccctac
     5041 ggctgcggct gctcgccgtc ctcgacgacg tcgctgaagc cacgtcgaaa gtgcacgaca
     5101 cgccgtaaag cgcgtgccat tacggcgtgt cgcgctcttt cgactcctgg tccaggtctc
     5161 gggagcgcag cttttcacgg cgctgagcag tcgtctgccg acggcgaatc tcgctgcgcg
     5221 ccacggcgat accgatcaga atgggcacga cgtatttcgc atgcccgacg atccagacca
     5281 gccagcccgg ggcgttccag tccggccacg ggatgctcgg caggttgatg tccggccagg
     5341 ggaggtcagg aaggtcgatg tccggcaacg gaatccgcgg caggttaagg tcaaggcccg
     5401 gcaacagccc ggcggagcgg gcgatcagcc acgcgacgat gaccggcccg acgaccttca
     5461 tgaccccgcc cgcgacgtgc cgggcggcat acagccccgg ccgacgggcc attctctcct
     5521 cacgcacggc caccggggac ccggcctccg gctccatgtc cacaccgccc agcccggtca
     5581 gggcctgggc ggaggcgccc tgtccttcga accagatggc ccggcgaggt gcgttgaacg
     5641 ccgtgtgaat cacgcggatc gccccgaggt gtgcaccctc ctcctcctcg ggcgtgagaa
     5701 cgaccttctc ctcgctcgtc gtcttggtcg cgaccagttc gccgtcgacc agccagagga
     5761 cctcgcgttt ggtgacggtg gcctcgcgcg agatcacctc gtgcacccgc ggtccgacct
     5821 ggaggcgcca cacctgaccc tgcttctccg ccacaccgcc ctcccctgcc gtcgtcactg
     5881 tcatacccag agtccagccg acacaggcga catcgcacat cggtcgaagg tcgacgcgcg
     5941 ctcccgcggc ctacgacttc ggtcgatcct tgcgttccca cgcgcgcgat agcgtgcccg
     6001 aaaaaggcgc gacgcccgcc cggtgaactg ccctatcgtg ggtgggctca gccgacccgg
     6061 cgggagtgga aggacacgaa agacaatccg atggagcaga tcaccgcgcg cctgatgaac
     6121 tgggcgagca tcctcgacgg ccagacccgg gcacaggccg agaccacagc ggccatgccg
     6181 ttcgtcttcc cgcacgtcgc cctgatgccg gacgcccacc tgggcaagag cgcgacggtc
     6241 gggtcggtca tcccgacgct cggcgcgatc atcccggcgg cgatcggggt cgacatcggc
     6301 tgcggaatga tcgccgtgcg aacgcaattc accgaggacg acctgccgcc cgaccggcgc
     6361 gccgtccgcg agttgatcga gcgcgccatc ccgctgtcgg gaggcgctcg caacacgacg
     6421 atcaggtgag ccggggctca tccccgggtc gatcggcact gcgtcgtatg tcgttgccgg
     6481 actgggcaat cggctcgcct tggattcggc gccccatggt gccggccggg tgcactcacg
     6541 cagctcggcc cgccggaccg acaccctcga gcaggtgcgc gagtcgatgg cgggcatcga
     6601 gttccgcgac agctcggcct tcatcgacga gattcccgcc gcctgcaagg acatcgacca
     6661 ggtgatgacg gacgccgcag atctggtcga gatccggcat agcctccgcc agctcgtcaa
     6721 cggcaagggc gagtgagtgg ctccacctag ccgcgccggg gctccacccc ggcgcggatc
     6781 aggccgtagg tcaccgactc cagcagggcg tcccaactcg cttcgaggat gttggccgcg
     6841 acgccgatcg tcgaccaggt cgtcgatccg tcggaggtct cgatgaggac gcgggtgatg
     6901 gcgtcggtgc cgtgctcggt gtcgaggatg cgcaccttgt agtcgacgag ctcgaggtgg
     6961 gagatctccg ggtatgccgt cgaaagggct tcccgcagag cctgatccaa tgcattgacg
     7021 gggccattgc cctcgcccgt cttgacgacc cgctcgccgc cggcgctcac cttgacggtc
     7081 gcttcggaca gggcgtcgtc gtcgccgcgg ctgtcggtga tgacgcgcca ggactcgaca
     7141 tcgaagaaat cgggcaggcc gccgttgagt tcacggcgca gcaacagttc gaagctcgcg
     7201 tccgccgcgt cgaaggtata gccgcgcatc tccttgtcct tgacggtcgc gagcacctgg
     7261 gccagcttgg ccgcgtcctg ctcgtcggcg ccgtcgaggg cgaggccgag ttcgcggctc
     7321 ttgagctcga tggacgcgcg accggccatg tcggagacca gcattcgcat gtcgttgccg
     7381 accgacttcg ggtcggtgtg ctgatagaga tcggggtcga ccttgatggc gctggcgtgc
     7441 aggccggcct tgtgcgcgaa cgccgacgac cccgtatagg gctgccgccc atagggcgcg
     7501 acattcgtga tctcactgat ggcgtgcgag attcgcgtcg cctccgcgag ccggtgcggc
     7561 tccaccaact cgcgcccctg cttgatctgg aggttggcga cgatggtgac gagattggcg
     7621 ttgccggtcc gctcgccata cccgttgatc gtgccctgca cgtgcgtcgc cccggcatcg
     7681 agcgccgcca tcgagttggc cacggcgcag ccggtgtcgt tgtggcagtg gatcccgatc
     7741 cgtgccccgg tcgcgccgac gacatcgccg acgacatcgg cgacctggtt cggcagcatc
     7801 ccaccattgg tgtcgcacaa cgcgatcacc tcggcgccgg cctctgcagc ggcacgcacg
     7861 gcctcgacgg catacgaccg attcgtcgta tagccgtcga agaagtgctc ggcgtcgagg
     7921 aagacccgtc gtccttcacc cctgaggaag gtgacggtgt cgcggatcat ctcgaggttc
     7981 tcctcgagcg tcgtccgcag ggcccgctcg acatgcccca cgtggctctt ggccacgaga
     8041 gtgacgacgg gagctccgga gtccaggagc gcccgcacga gcggatctga ctctgcccgc
     8101 ccaccggccc ggcgggtcgc gccgaaagcc gtaagcgtgg cgttcttcag ctggagctct
     8161 gtttgagctc gccggaagaa ctcggtgtcc ttggggttcg cccccggcca cccgccttcg
     8221 atgaaaccga cccccaactc gtcgaggtgg gcggcaatcg cgagcttgtc tgtcaccgag
     8281 aggttcaagc cctcctgctg cgcgccgtcg cgcagagtgg tgtcgtagac gtgcagctcg
     8341 ttcatgtcgg tccttggtcg tggtcgtcgg tcggtgcggc gttcgcgggc aagaaaaaac
     8401 ctcccgggca gggaggtcgg cgcaacgagg aggtctcgtt gcgctattcg ataatgatgc
     8461 tcgcgaaggt cacgccgtca ctcaagcaca ttcctcacgc gattgccaag ggctgtccgt
     8521 atgccggtgt gtcaccgccg accgcgcacc gctgccctgc gctgggccgc gcggtcccgg
     8581 cgggcccgct cggtgcccat ccggtcggcc caccgcgaga agaactggct gagctggatg
     8641 aagacgcgcc acccgacgag cagcaggccg ttgaagacca gcgccacgac gatgaacgag
     8701 gttgccgtgc cggcgtcgat gaccacgcgc agcagcatcc cgacgatcac ggtggccagc
     8761 cagatgacaa ctcccggccg caccagccac ggtgccggcc ggtcgcgcat gagcagcacc
     8821 ccgagccaac cgaccccgag cccggccagg aagggccacg ccgtgtcgaa ccagccgccg
     8881 agactcaggc cctcgtcgtg cgcggctctg ccgatcagtg cgaagacccc gacgcacgcc
     8941 agatcggtga cgagcgagcc gagggttgtc cccacacggc gaacggggag tgcgcgggcg
     9001 gcggctgacg taggcatggc tggtcctgat ccgattggtg cccaaggcat gtgtccctgc
     9061 gattctaggc gccgcacgcg tcgggcaccc gcggtgctat cgagttggtt tctctaagag
     9121 cctatttcga cctagcagcc tatttccacg agttcttcca agacagtcgc gacgccgtcc
     9181 tcgtcattgg tgcgacaccg atcggtcgtg gcggcgagca cgtcggggtg cgcattgccc
     9241 atggcgaagg accggccggc ccaggtgagc atcggcaggt cgttgggcat atccccgaac
     9301 gcccacacgt ctgcggccgc gaccccacgc tcggcgcacc accgctccag gccggcggcc
     9361 ttggtcaccc cgggcgggtt gagctcggcg agcccgaagg cccccgaata cgccagcaca
     9421 gcggcctcgc cggccacctc ggccacggcg gcgaggaagg cgtccgtcgg gtcaccgggc
     9481 gcgagcgcca gcagtttgcc gatccggtgc ccggcgtcct ggctggcgac gagcgactcg
     9541 ataagcggga tcatcgtgcg cgcgtcggca tcctcctcgt gcgggttcgg ccagctctcc
     9601 tcgacgaagc agccgaccgc ggtctcagcg gcaaaagtga tatgtggcaa tcgctctcgc
     9661 agatcggcca cgacctcggc gaccgctgcc ggcgcgaatg tgtgcacctc caccaccgca
     9721 cgcgaaacca cgtcatagac gaaggccccg ttgccacaga tcgcgatgcc gtgcgcgccc
     9781 accacgtgac tcaggtcgtg caaccaccgc ggtggccgcg ccgtgacgag caccgtgagg
     9841 atgccggcct cggcgaccgc cgcgagcgcc gccgcggtgc gggccgagac gctgccgtcc
     9901 gagcgcaaca gtgttccgtc gagatcggtg gcgatcactc ggggccgccc ggccacgggc
     9961 ggcctgagaa ccggcgggac agacggcata ccaggcagca gcgacccgat caggcgagct
    10021 gggtgagcca gccgtgggtg tcctcgaccc ggccatactg cacgtcgagc aaccgcgagc
    10081 ggatcgcatt ggtgacctcg ccaccggcct cgccggccgt cgacggcgcc tggccgccct
    10141 cccagcgcag ctcgcccacc ggtgtgacga cggccgcggt gccgcacgcg aagatctcga
    10201 cgatgtcgcc ggaccgcacc ccgtccttcc actcctcgat ctcgatgcgc cgctcctccg
    10261 ggtcgagccc gagttccttc gacaggtcga ggatcgaact gcgcgtgacg ccttcgagga
    10321 tcgtcccggt cagctcgggc gtcacgagcc ggtggtcctt ggtgacgaag aagaggttca
    10381 tcccgccgag ctcttcgatg taggagtgcg tcgaggagtc caggaagacc gcctgatcgc
    10441 acccgtgctc ggcgccctcc agctgcccgg cgagcgagga cgcgtagttg ccgccgcact
    10501 tcgccgcgcc cgtgccgccg gcaccggcgc gggcgtagtg ctcgctgatc cacagcgtca
    10561 ccggcttcag gccgccggcg aaataggcgc ccgccggcga ggcgatcacg ctgtaggtca
    10621 cctcggccgc cggccgcacg ccgaggaagg cctcgctggc gaacatgaac ggccgcaaat
    10681 agaggctctt ctcccccgac tcgcccggcc cgggcaccca cgccttgtcg gcctcgacga
    10741 tcgcccgcag cgaggcgagg aaatcctcct ccggcagcgg cggcagcgcc agtcgcgtcg
    10801 cgctgcgcgc catccgctgc gcgttcgcgt gcggccggaa gccccacacc gatccgtcct
    10861 cgtgtcggta ggccttgagc ccctcgaaga tctcctgtgc atagtgcagc acggccgccg
    10921 acggcatgag ggagatgggc ccgtatgccg tgaccgcccc gtcgtgccag cccgcgtccg
    10981 tggtccacgt ggccgtgacc atgtggtcgg tgaagtgctt gccgaacccc gggtcggcca
    11041 ggatcgccgc ccggtcggcg tcgctggccg ggttggcgtt gggcgtgtgg gtgaacgaga
    11101 gcgcggtcat cgcaggcatc ccttcggtcg acagggcctt caccaaccgt acttggtcgc
    11161 cgcacctgcc gccgccacgg atgtcgacgt cgctggccat cggtcgtggt tgtgatcacc
    11221 accgacaccg atagtcaacc cggtgcctgt ctggcccggt gcttgtctgg gccggtggtt
    11281 gtctggccgg gtgcttgtcc gccgggtcag agtcgggcgg cgatggcgtc gccgacctcg
    11341 gtggtcgagc gggcggcggt gccacgttcg gcgagatcgg cggccaccgc ggcgtccacc
    11401 ctggcggcgg cgtcggcata cccgaggtgc tcgagcagca tggcgacgga caggacggtc
    11461 gcggtcgggt cggccttggc ctggccggcg atatcgggag ccgacccgtg caccggctcg
    11521 aacatgctcg gtgcggcgcc ggtcggattg atatttccgc tggcggccag cccgatgccc
    11581 cccgcgatcg ccgcggcgat gtcggtgatg atgtccccga agaggttgtc ggtgacgatg
    11641 acgtcgaagc ggcccgggtc ggtggccatg aaaatcgtcg ccgcgtcgac gtgctggtat
    11701 gccgtctcga cgtccgggaa ctccgcgccc acctcttcga cggtgcgccg ccacaggtgc
    11761 ccggcgttga cgaggacgtt gtgcttgtgc accagggtca ggtgcttgcg cggccgcgcc
    11821 tgcgcccggg cgaaggcgtc gcgcacgacc cgctcgacgc cgaagcgggt gttgacgctg
    11881 acctcggtcg cgatctcgtg gggcgtgccc tcgcggacgg tgccgccatt gccgacatac
    11941 ggcccctcgg tgccctcgcg cacgacgacg aagtcgatgc cgttgggggc gaccgccgag
    12001 acgtcgagcg ggctggcgac gccggggaag agcttggccg gccgcaggtt gacgtagtgg
    12061 tccagcgcga agcgcagcgg caacagcacg ccgcgctcca gcaccccgct cggcacggtc
    12121 gggtcgccga tcgcgccgag caggatcgcg tcctgcccgc gcagctcttc gacgaccgag
    12181 tccggcagcg tctcccccgt cgcgtgccag cgcttggcac cgaggtcgta ttcggtgcgc
    12241 gcgaaggtca gcccctcgct cgcgccgaca gcctcaagca ccttcaggcc ctctgccacc
    12301 acctccggtc cgatgccgtc cccgccgatg acggcgaggt cgatggtgcg ggaaccgggc
    12361 gacatggcgg tgtcggacat gtcgcaaccc tagggcgggt tctcggcagg tgatacggct
    12421 atctcgccat ctggcgcggg cgaccgtcag tcgagcgtca cgacttccgc gagggtggcg
    12481 tcgacctcgg cggcgatggc gctcagcacg ggggccggga tcgccgagtc gagggtgagg
    12541 acgacaagag ccgcgccgcc ttcttccttg cgggagagct gcataccggc gatgttcacg
    12601 ccggcgtccc cgagcaggcg accgaaggcg ccgatgacgc cgggccggtc gacatacgac
    12661 aggaacgcca tgtgcgtgct gatcgggact tccaggtcga agccgttgac cccgatgatc
    12721 ttctgcacca tcttcgggcc ggtcagcgtg ccggcgacct cgacgatcga gccgtcggcc
    12781 agggtgccgc gcagggtcgt gacattgcgg aactcctccg ccaccgggtg ggtgatcagg
    12841 cgcgcctcgc agccgcgctc ctgggccatc agcggtgcgt tgacataggt gaccggctcg
    12901 ttggtgatgt cggtgaacag gcccttgagc gccgagagct tccagatgga gacgtcgaac
    12961 tcggtgatct cgccgcgcac gtcgatgtcg agctgcaccg gcaccgaccc ggcgacggcg
    13021 gtgaagatcc ggccgagctt ctccacgagg tcgatacccg ggcggacctc ctcggcgatg
    13081 acgccacccg ccacgttgac cgcatcgggg acgatgtcgc cggccagtgc caggcgcacc
    13141 gacttcgcga ccgagattcc ggccttctcc tgcgcctcct ccgtcgaggc gccgaggtgc
    13201 ggggtgacga cgaccttctc gtggtcgaag agcggcgact cggtggtggg ttccgcggcg
    13261 aagacgtcga gcccggcccc gccgatgtgc ccggacgcga tggcgtcggc cagcgcggcc
    13321 tcgtcgatga tgccgccgcg ggccgcgttg acgatgatga cgcccggctt ggccttggcc
    13381 agcgcctcgg tgccgatgag cccgagggtc tcggccgtct tgggcaggtg gatcgagatg
    13441 aagtccgact cggccagcaa ctcatccagc gtgaccaggc gcgcgcccag ggcgccggcc
    13501 ttctgcgccg agacgtacgg gtcgtaggcg aggatctcca taccgaagcc cttgagccgc
    13561 tcggcgacga gctggccgat gcggccgaac ccgacgatgc cgaccttctt gtcgagcagt
    13621 tcgacaccgc catacatgct gcgcttccac gccccgccct tcagcgcctg gttggcgggg
    13681 gcgatattgc gggcggcggc gagcatgagg ccgacggcca gttcggcggc gctggtgata
    13741 ttcgacgtgg gcgcgttgac gaccatgaca ccggccttgg tcgcggccgg gacgtcgaca
    13801 ttgtcgaggc cgacgccggc gcgggcgatg accttcaggc tcttggcggc ggcaatggcc
    13861 tcggcgtcca tcttcgtggc cgaccggatg aggacggctt cggcctccgg cagagccgcc
    13921 agcaactcgg agcgattggc gccatcacag gtgcgcacct cgaagtcggg accgagcgcg
    13981 tcgatggtgg cgggtgagag ttcctcggcg atgagcacga ccggcttggt cacgatcttt
    14041 gtccttcggt gggtgtgcgt atgcccgggc tgaccccggg tggcccgtcg ggagggccgg
    14101 gacggcacga cgctgtgcat ggccagctta ggtgccgtcc aggactcggg atgcgccgtt
    14161 caccaggtga ccaccccggc tcacggggcc gacgcatcgt catccccgag atgggtgtca
    14221 tccgttgtcg cagtttgcga ttcaacgcgt ccgcgaacca gcctcgtgac caagaccaca
    14281 agggtcacca gccccagggc gatgagtagc caccaccagg gcaaggtgcc ccgcacgacg
    14341 tcgagggccg cgaagcccgc tgtcgtatag accccggccc acagcagcga accgaccacg
    14401 gcggccggcg tatagcggcg taggtccatc cgcagggccc ccgcggcggc gttgagcacc
    14461 gtttggatcc cgacggtgag gaaggagagg gtcaccaggg gcgcgccata ccgcgacatc
    14521 agacgttcgg cgcgctgcac cccgggccgg tcgatgtgcc ggctcaggcg cgccagccac
    14581 gtgtcggccg ccgtcgggtg ggcagcgcgc gtggcaccgg cgcgcagacc tcggccggcg
    14641 gcatacgtca cgtgcgcccg caccatcgcc ccgcagaaga acagcaggta gacggcccag
    14701 aagggccacc cgtccaccag ctcgtccact cctggaccct atccgcagca ctctggcacc
    14761 atcgtcgtca tgcgagtcgt cgtcaccggc gggctgggca acctcggccg cgcagtcgtc
    14821 tccgccctgc aggacggggg gcacgaggcc gtgatcgcga gccggcgcac gggggtggac
    14881 ctgcgcaccg gcgccggcct ggccgagtgc ctgcgcgacg cggacgcggc cgtgcacacg
    14941 gcggacagcc tgcggccgtg gcactggaag acggtgacgc tcgggggcac ccgcaccctc
    15001 gccgagaccg cggccgggct ccccgctccc cctcacctcg tctacatctc catcgtcggg
    15061 gtggaccgca acccttaccc ctactaccgc gcgaagctcg cggccgagca ggtcctcgcc
    15121 gacgcgccag tcccggcgac cgtcgtgcgc gccacgcagt tccactcact cgccgcggtc
    15181 atggccggta tgcggctggg tccggtcgcc gtgggcgtgc gagggctgtc catccagccg
    15241 gtcgacatca cgtgggtcgg ccggcgtctc gccgagatcg ccgtctcgtc tcggccggac
    15301 ggcttcaccc gagcccgcga gttggccggc cccgatcgct tcgaggccgc cgagatcacc
    15361 gacctcgtcg ccgcgcacct cggcacgtcg gctccccgcc ggctcgagat gcccccgctc
    15421 ggcgcgaccc tcaaggcctt cgcggccggc gccgtgctgc cgcgggacga cgccgagatc
    15481 ggcggtgagc gattcacgga atggcttgcg cgccaaggct cccggctgcc gcgccgatga
    15541 gttcgcgctg ccggtggggt cagcccaggt agatcagacc gggtccttcg tcgcgcagcg
    15601 gtatgcccag tgccgcaagc gccggcgccc acgacccggt gatcgccggg tccgacccct
    15661 cgaagccgtg atcggccgtg aggatgaagg tcacctggtc ggtcacgccg agcccgtcga
    15721 ggtggtcgag gaaggcgacg agccgagcat cggcctgccg caacgcatct cgcgccatcg
    15781 acgaacgcgg accgccacca tgatgcccgg cgtcggtgac gacattggcc caccaggtca
    15841 gggcgggcag gtggtcgggg ccggcgaaga gctggcgcat ctgctgcagc ccgagatcgt
    15901 cgacctgcac gccccaccgg aaatagctgt cctccaggtg ctccggttcg tggacgaacg
    15961 gtgaggtatg cggatcgggc aactgatccc cgaggccgcc cgctccgcgg ctgctgccgc
    16021 ttgcgcggat gatctgcatc gtcgaatagt cggcgccgcg gtcgatcgcc tcgtcgacgc
    16081 tcgcggtgcg cggtcgcagg aagcgcgccg ccgcgagagc agcgtcgtcg gcatcggccg
    16141 ccacgtccgg gacggggatg tggtcggcga ccatctcgaa gacggtacgg accgagtcgt
    16201 gaagccattc gctgctgcgg tgccaggtgg tctcgtcgtt ggggacgacc cgctcgcccg
    16261 tcgcccggtc gacgtagacg ttgcccagca cgccgtgccg gcccgggccg acgccggtga
    16321 ggatcgaggt gtggttggtc agcgtgatgc tggggaactg cgcgatcgcc ccgccacgca
    16381 gggccgtgcc gcgggcgatg agccgggcga cgccgggcag gtcgccggac tcggcgaggt
    16441 gcagcaggtc gccgcagtgg ccgccgtccc agaggatgcc gacgacggga ccgatcggcc
    16501 cctcgttcag ataccggcgc aacacgcgcc cgtcgagcgg gtcgcccttg gcgtcgcgca
    16561 ggtcggactc gggcacgccc gcgagatagg ccagcgtcgg cccgacatcg atcaggcgcg
    16621 cccattcgtc gacgaggccg cgggcttcga cccgtgcgcc gctgacgatc agcggcgcac
    16681 gtgactggat gacatcgagg gagccgtgct cgcccacgtg cccgccctcg tcggggaagt
    16741 agtggccggg cgtgtgcacg atcgcgatgt cggggctgcg gtccggatcg gcgaagaaac
    16801 tcaagagccg ttccgccgca agcggatacg cattgtgcgt ggagaccgga ggcgacgggt
    16861 gggccagctc cgcggcatac ggcaagaagg cgagcgggtc ctcgctgacg atggggtcgc
    16921 gtccggcgag gacggtatgc cgtccgcccg gctccagccg cacccgcccg aggtggttgg
    16981 cggcatgcgc aacgggggtg ccgtccgggt ccggcgcgac ccagacgacg agatcgacga
    17041 cggcggcgag atccggggtc gtcaacgccg tgacgacccg ttcatggtcg gggtggagag
    17101 cacgcacggc cgcgagccta cttgccgctc ccgaggaacc tctgctagca gaggttggtg
    17161 acatcagcag acctagggac gtgtgctgat gtgactagcc tctgctagca gaggttacgt
    17221 acaaggcgcg ccggtcaggc ggcgtaggag tccttgcggg gacggccacg gggccgcttg
    17281 cgggcgacga cgacaccatc gacgaagtac tgaccacccc aga
//
LOCUS       DAOPJG010000052        17304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3476_length_17304_cov_7.895298, whole genome shotgun sequence.
ACCESSION   DAOPJG010000052 DAOPJG010000000
VERSION     DAOPJG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17304
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3476_length_17304_cov_7.895298"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1390
                     /locus_tag="PKU75_06795"
     CDS             134..1390
                     /locus_tag="PKU75_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF80621.1"
                     /translation="MVMDRAQAQRHTVRTLMLTQVLGGVGLAAGATVGSLLAERISGR
                     TDLAGLGGTFQTLGSALLALPIAAVAHRLGRRPSLTMAYVVAALGALGIVTASAISSF
                     ALFLLGSALLGSATAANSAARYAAADLALPERRGRDLSLVVWVTTVGSVFGPNLAGPA
                     APLARTLGLEVLAGPFVISTVTLTSAALVMTWLLRPDPLLLSRTLAGEEYEHQKGSVG
                     RGFAAIRRHPAAALGIGVMALAHVVMVGVMVMTPIHLVHGHASLAVVGFVISGHILGM
                     FAFAPVMGWGVDRYGAPPVALTGGLILVVAAILAGLSEAGFSALLTVALFLLGLGWSA
                     AFVSGSTLLVGPLTVAERPGGQGLADLVMGLTAAAGGVVAGIVVQHWGYAWLAHIALT
                     GAAAIMILAAMALSAERGAGAGRTVD"
     gene            1400..5002
                     /locus_tag="PKU75_06800"
     CDS             1400..5002
                     /locus_tag="PKU75_06800"
                     /inference="COORDINATES: protein motif:HMM:NF037405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80622.1"
                     /translation="MTAVPAPTPGSPEAAAQQVSRLRAEIVQQFGPNTLLWSDQAIVL
                     DVTHAHPGGLAKLLASKDTLLSELYRESATQQRAISKARAIRAKEQELARERGVPGCY
                     LAIGRATWTERRGPKPNAPVFLRRLELIPESPAQQEFRVVLTGEAEFNPALRGFLESV
                     GGTDIPFDDLVRMSMRPSGFDPAAAYEALGRSWPGPPGWSIARDMWITTFPYAKLPAV
                     SDFTRVAAALEEHPLLDTLVRGAATGPAPGRTHEPADPELVTVLDADPGQIAAIEAVR
                     AGESIVIDSPPGTGKSQTIVCIAADLARSGRSTLVVAAAADSRSAVTGRLVHCGLGSL
                     VREPGTGSAPAAHRPSQFARASQVQSAWTSAGRELSEHVARMHRGREPWSLSVDEIQE
                     RIAAVCEAPEPPRSKIRLSGPHLGALGPAEAERWMTTLRGVAERGAWPSGEEPADPWW
                     GAAIRTDEDAERARLAAKALGASGLAQFDAVFTEVFAGVSVPPLRTLGQHGAFIAEMA
                     RIGQVLEVFRLGVFDAPLEEWTAHTSGGTFERWRHGRAVKALLRPGAPHHDVDPLLHS
                     ALQARPVWQQVRGSQIMPGQVAGLGRAREAYDKLAAHASYLGERLRGHPDLLAERLPQ
                     LAARAADLSAQRSRLEVLPAVTDDLDAARTAGLGELIEDLAARRLSPEHVAAETEFVW
                     LASVLAQTTASDSGYARVTGDAVRAAVTRFRENDRALQRAAAAGIIAERGEGKPLICV
                     CSPHTVGLYLSRKAHFDVVIIDDAQALATADGISALARADQAVVVGDSHLPGPTFFTA
                     TAGGAAAKGSPSSSLLTEAAAVWPVHRFAWHYRSRDQRLTESPVATAYPELRTFPSPS
                     DTGVVRVEAAPVTEHDHGLVAQAVRAVVTHARLQAKESLAVLTVDPELAQRIRDGIRA
                     TTDPSVADFLGEQGGEALVVLDAAHATGVVRDAAIVVLGQQTPDLVAGAAGARLLTAA
                     LTRARARQTVLHTLDAAKLADAAGDGPARLGARILADLLANDRPKVTTTSESTLTRDL
                     ARRLKSRGLRVREGVAPGMVDLAVRDPFTRGPVALAIQIDGPAYAALGGNRMRDRLVQ
                     EQLRRLGWRPLRILTVDLFRDPAREEARIVAAMERMAAGDMRTGPITGKGARRASRPA
                     TGRAADGAADQPAATGRPEQTRDDTDAGWGERPASGGDPAHDRWLQENRPPHWE"
     gene            complement(5163..5516)
                     /locus_tag="PKU75_06805"
     CDS             complement(5163..5516)
                     /locus_tag="PKU75_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HPF80623.1"
                     /translation="MKGFKDFILRGNLIELAVAFVMGTAFAAVVTAFTEIIIEGLAKA
                     GGAPNFDDWKPGGWTSVGPFLTALVAFLIIGFMLYFFVVKPYEAARDRFLAEDEQVSD
                     EISLLTEIRDALTRR"
     gene            complement(5668..6327)
                     /locus_tag="PKU75_06810"
     CDS             complement(5668..6327)
                     /locus_tag="PKU75_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80624.1"
                     /translation="MLSPTKTKGKLMAPDSPFAPVIGAGRRAAWRRTLLRRIAALLIL
                     AAGLALLILRPGADGVPAAAPTPSASATEEAGGVGIPGLDLPGGGDDPVPTAPATPRT
                     SALGATALPEDRVGVVLPVPAALAVRLRPGDTVDLYAAGARKPMARDAEVVDVTRPGE
                     GSGTDPATGASPGGEASVFLAIDSADVEHVSAGQRASSAGSPMLVVLASRASDQARRI
                     P"
     gene            complement(6340..7176)
                     /locus_tag="PKU75_06815"
     CDS             complement(6340..7176)
                     /locus_tag="PKU75_06815"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HPF80625.1"
                     /translation="MTANDQQAEIGVIGGSGFYSFFEGAPHHRVETPFGAPSDEIAIA
                     DLGGRRVAFVARHGAHHQFAPHRVNYRANLWALRSLGVRQVLSPCAVGSLRPELGPGS
                     LVVPDQVVDRTSGRAHTVYDEPGPVVHVSFAHPYCERGRAAVLSAVREDGRLSATDGG
                     TLVVVGGPRFSSAAESRMHQAQGWSIVGMTAMPEAALARELAMCFTTVALVTDHDAGI
                     DGVPAVNHADVLTVFAQHMQTLKDVLRSAVATLPPHEPDESATCPCRRALDGLKLPFE
                     LP"
     gene            complement(7219..7569)
                     /locus_tag="PKU75_06820"
     CDS             complement(7219..7569)
                     /locus_tag="PKU75_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPF80626.1"
                     /translation="MPTYAYACADCGHAFDIVQSFSDDSLTVCPECEGRLRKQFNAVG
                     VVFKGSGFYRTDSRSSSTSSVPSSGGSSSGSSSNSSSGGSSGSGDSSSSSSASSSSTS
                     STPAKSTSAVSTGS"
     gene            complement(7668..9242)
                     /locus_tag="PKU75_06825"
     CDS             complement(7668..9242)
                     /locus_tag="PKU75_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPF80627.1"
                     /translation="MLPLAGDAQTGFTLGELTTALLVGSLTLIVCVVAIRLSNRTGLP
                     SLLIYLGIGLLIGENGLGIRFEDEQLTLVLGYAALVLILIEGGLTTRWDGIKDSIAPA
                     GLLATLGVGVSVAVVAVAAHFILGWSWNAALLVAAIVSSTDAAAVFSVLRSVPLPRRL
                     TGMLEAESGFNDAPVVLLVSALALAAANPGEAHSPWAILGLAATELAGGAVIGLVVGY
                     AGGQLMRRSAAGSSGLFSIGVIAVAVLAYAAAASLHASGFLATYLAALVLGNLGLPHQ
                     PAVHAFATAMGWLAQIGLFVLLGLLASPAGFAEQLVPALVVGLVLLLLARPLSVAASV
                     SWFGFSLRQQAFLSWAGLRGAVPVVLATVPVIERAPNVDWIFDLVFVLVVIFTLLQAP
                     TLPWVARRLGVTEAHRTRQLTVESTPVEELNASLLTVDVGPTSMLHGVAVFELRLPVG
                     ADVTLILRDGHNIVPTPRTKIHHGDQLLVVTTDAQRSRVEELMRAISEGGRLAEWTKK
                     DRLKVRKRPGRPATFG"
     gene            complement(9285..9857)
                     /locus_tag="PKU75_06830"
     CDS             complement(9285..9857)
                     /locus_tag="PKU75_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPF80628.1"
                     /translation="MGLSVKQQVRAQRRAARRAIAATRDLAADGEGIARHTLALADNL
                     AIGPGEVVTAYEALPAEPQTQAVCRALTQRAVHVIVPITLASFDLDWHELDDPDRTPL
                     GLDAVAACRLLLIPGLSVDRHGTRMGQAGGCYDRTLPRARPDAVVVTLLHPGEFLDAT
                     LPADHWDQPVDAVVTADGPAWTRHGQRHRP"
     gene            9914..11296
                     /locus_tag="PKU75_06835"
     CDS             9914..11296
                     /locus_tag="PKU75_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPF80629.1"
                     /translation="MSDAGLAHATTAMAERGASPAAIATFDRFYRMLESGEQGLIPES
                     TIAPLTDVTTLEGVAASEADRRAALAKVVVIKLNGGLGTSMGLSGPKTALKVRGELTF
                     LDIIAQQTLALRQRYAVDLPLLLMNSFRTREESLEILAAYPDLPVAGLPLDFIQNAEP
                     KLTADTLEPVSWPADPELEWCPPGHGDVYVSLQGTGLLYALRQRGIRYAFLSNADNLG
                     ATCDPDIAAWLLANDIPYAAEVCERTANDRKGGHLAVRTRDNQLILRDSAMVVPGEDE
                     HFQDNTRHTLFHANNLWVDLDELAALMERSGGLVPLPVIVNRKTVDPTDPSSTPVIQI
                     ESAMGAAIEVFPGSQAIAVPRKRFRPVKSTNELLLVQSDIFDLDADANLVSRITHSEP
                     AISLGKPYKFVPDYLARFPQGVPGMRECTSLTVDADATFGAHVTCVGDVTISGEPRVI
                     PDGATLTGTL"
     gene            11303..12529
                     /locus_tag="PKU75_06840"
     CDS             11303..12529
                     /locus_tag="PKU75_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPF80630.1"
                     /translation="MRSVSEHREAILAALPPRAGAEVPLADCLGLITTADVVARVALP
                     GFDNSAMDGYAVRAADVAGASPEGPAALTVVGEVAAGADAASVTVGAGQAVRIMTGAM
                     LPAGADAVVMVEATDGGLDVVQVYAAVPHGRSIRTAGEDVAQGALVIPAGTRVNARTL
                     ALAAATGHPTLPVRPRPRVAIVSTGDELIPPGEPLSPGQIHESNSVMLAAACRSRGAE
                     VVATVAAGDEPEAFVGLIEDLASECDAIVSSGGVSMGAYDVVKAALREHGIEFVQVAM
                     QPGKPQGFGLFGEHRVPVFALPGNPVSSFVSFEVFVAPALEAMMGGTHERRVVTGVMG
                     HALTSPRGKTQIARAVTTPEGGAWVVNPVVGQGSHFVADLVRANSLVIVPADAESLAA
                     GDEVEVWLLSDPGPIP"
     gene            12526..13050
                     /gene="moaC"
                     /locus_tag="PKU75_06845"
     CDS             12526..13050
                     /gene="moaC"
                     /locus_tag="PKU75_06845"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPF80631.1"
                     /translation="MNELRLTHVRADGSAHMVDVSAKAVTAREASAAGRVLLSEKAVA
                     ALRDGAVAKGDALAVARIAGIQAVKRTPDLIPLAHPIAVHAVEVDLTVADDGVDIRAS
                     VRTADRTGIEMEALTAVAVAALTVIDMVKAIDKHGRITDIRVTAKSGGRSGGWAETPT
                     ETTRTRPQPSQEMP"
     gene            13047..13556
                     /locus_tag="PKU75_06850"
     CDS             13047..13556
                     /locus_tag="PKU75_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPF80632.1"
                     /translation="MTDTAQDRPPPLRGVRAVAITASTRAYSGVYPDRGGPIIAETLQ
                     SWGALVAPTVVVPDGDAVATALREAVTAYDLVITTGGTGCAPTDLTPEATRSVLTREI
                     PGIAEAIRADGLANGVAGAMLSRATAGLAGRCIVVNLPGSTGGVRDGLRVIAPILGHL
                     VEQLRGGDH"
     gene            13557..14654
                     /locus_tag="PKU75_06855"
     CDS             13557..14654
                     /locus_tag="PKU75_06855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80633.1"
                     /translation="MRSRLWPVDLAATLADGGQLGLRPLGRKDREDYVLLRAANTDWL
                     RPWDPTSPDGIPRTVSFTSLLSEQKAAARAGSLLPFALTVDGALAGQLNVSNIVRGAF
                     RSCTMGYWVGREFAGRGIAPLAVAVAGDYAIAAAGLHRIEINIRPENVASLAVVHKLG
                     FRSECIRPRYLHIDGAWCDHLSFALTTEEIGPGGLRARLAGLESGRPRDAVAIRPATA
                     EDLPWLTCQWRRAWGSTQVAIGDDLLDLRDHPARVAAAAGELLGYLVYRDLGGARGIL
                     ALEAVHPGRGVGQALIEDAAEDTRLPLVLTTTNDNLNALGVYQHLGFVITDVRPGAID
                     RARERKPTIPLLGDNGLPIRDEIVLTLPQSD"
     gene            14742..15689
                     /locus_tag="PKU75_06860"
     CDS             14742..15689
                     /locus_tag="PKU75_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80634.1"
                     /translation="MQPSSLIFLVVIAIWAAYLLQHWLGRREDLATARSVDRFSESMR
                     ILDRAARDQRAEPARAEVSSTRLHPRQVTVTRAAALLAATPAAATAKTAARTFGATAV
                     SGLAGRRPAATGRRRAGRATRGLFVLGAGALTLVTLVLATMSVVGWWVPILALVVTAG
                     AQWWLRRAVVAERAARRAAPARPTMQRPATTTQRPVQRRAAQPPARVAQATAGGRVET
                     VGDDTADAPATVQETPAARETLYDLAAIEEAEAESARARAAAEGWNPVPVPPPTYTLK
                     AKAVPAEEILGDEDDDVASPFSGDVTDLSEYAERARLSG"
     gene            complement(15693..16874)
                     /locus_tag="PKU75_06865"
     CDS             complement(15693..16874)
                     /locus_tag="PKU75_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPF80635.1"
                     /translation="MSEPHTEPGSAVPKGVRDASEWAWRFLLIAAALALFGFLIGHLS
                     EIVVPIIVALLLAALLHPLFWRLDQHLPRGLAAAITVLGTLAVLSGLMTFVGSQFAEQ
                     LPDMTDQVGEGIDQIRTWIRDTFNISDSQFSEYFQKAREALSNSSSDLGGTAAKFGLT
                     ATHVVAGFFISMFALFFFLLEGPHIWAWVVRLFPRSARARVASSGEIAWGQLKAFCKA
                     TILVAAVDATGIGIGAAILRVPFASGIALLVFLGAFVPVVGALMSGIVAVLLALVALG
                     PVQALIMLGIVIAVQQIESHVLQPFLLGRAVRVHPLAVILSIAAGVIVWGIVGALIAV
                     PVAAVVNAVGHHLLADDEPAPDKAEDLLPAGERAQVDGEVAAADARARQAAADDALGS
                     E"
     gene            16990..17062
                     /locus_tag="PKU75_06870"
     tRNA            16990..17062
                     /locus_tag="PKU75_06870"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17023..17025,aa:Ala,seq:cgc)
     gene            complement(17180..17304)
                     /locus_tag="PKU75_06875"
                     /pseudo
     CDS             complement(17180..17304)
                     /locus_tag="PKU75_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6112 family protein"
BASE COUNT         2512 a         6194 c         6180 g         2418 t
ORIGIN      
        1 gtggtggtca tgaccagaac tctcgcccgt cggcacggcc acgctccctg cgctccgtca
       61 cgacctgcac ctgacagtcg tcacgggtgg tgcgaagtcg gggagcgcac cccatagggt
      121 gaggccggtg cagatggtca tggatcgtgc ccaggcgcag cggcataccg tccgcactct
      181 catgctcacc caggtcctcg gcggggtcgg tctcgcggcc ggtgcgacgg tgggttccct
      241 tctggcagaa cgcatcagcg gccgcaccga cctcgcgggc ctcggtggca cctttcaaac
      301 cctggggtcg gcgctgctcg cgctgccgat cgccgccgtc gcccaccggc ttgggcgccg
      361 gccctctctg accatggcgt atgtcgtggc cgccctcgga gccctcggga tcgtcacggc
      421 gtcggccatc tcatccttcg ccctcttcct cctcgggtcg gcgctgctgg gctcggcgac
      481 ggcggccaac agcgccgccc ggtatgccgc cgccgacctc gccctgcccg aacggcgtgg
      541 gcgtgacctg tccctcgtgg tgtgggtcac gacggtcggt tcggtgttcg gcccgaatct
      601 cgccgggccg gcggcgccgc tggcccgaac gctcggcctg gaggtcctcg ccggcccgtt
      661 cgtcatcagc acggtgacat tgacctcggc ggcgctcgtg atgacctggc tgctgcggcc
      721 cgacccgctg ctgctgtccc gcaccctggc gggggaggag tacgagcatc agaaggggtc
      781 ggtcggccgc gggttcgcgg cgatccgccg gcacccggcc gcggcgctgg ggatcggggt
      841 gatggcgctc gcgcacgtcg tgatggtcgg ggtgatggtg atgacgccga tccacctggt
      901 gcacggccac gcgagcctcg ccgtcgtcgg cttcgtcatc agcgggcaca tcctcgggat
      961 gttcgccttc gcgccggtga tgggctgggg cgtcgaccgg tatggcgccc cgcccgtcgc
     1021 gttgaccggc gggctcatcc tcgtggtggc cgcgatcctt gcgggactgt cggaggccgg
     1081 cttctcggcg ttgctgaccg tggcgctctt cctgctgggg ctcggctggt cggcggcgtt
     1141 cgtcagtggc tcgaccttgc tcgtcggccc gctgacggtg gccgagcgcc ccggcggaca
     1201 gggtctggcc gacctcgtca tggggctgac ggctgccgcc gggggcgtgg tcgccggcat
     1261 cgtcgtgcag cactggggct acgcctggct cgctcacatc gccctgaccg gggctgccgc
     1321 gatcatgatc ctggccgcga tggccctctc cgccgaacgc ggcgccggag ctggtcgcac
     1381 ggtggattag ccttctgccg tgaccgccgt ccccgccccg acacccggct cgccggaggc
     1441 ggcggcgcag caggtctcgc gcctgcgcgc ggagatcgtg cagcagttcg gacccaacac
     1501 cttgctgtgg tcggaccagg cgatcgtcct cgacgtcacc cacgctcacc cgggcggcct
     1561 ggccaagctc cttgccagca aagacaccct gctcagcgag ctgtatcgcg agagcgcgac
     1621 gcagcagcgg gccatcagca aggcgcgggc catccgggcc aaggagcagg aactcgcgcg
     1681 cgagcgcggg gttcccggct gctacctcgc catcggtcgc gccacgtgga ccgagcggcg
     1741 cggaccgaag cccaacgcgc cggtcttcct gcggcgtctc gaactgatcc cggagagccc
     1801 cgcgcagcag gagttccggg tcgtcctcac cggcgaagca gagttcaacc cggccctccg
     1861 tggcttcctc gagagcgtcg gcggcaccga cataccgttc gacgatctgg tgcgaatgtc
     1921 gatgcgcccc agcgggttcg acccggctgc agcgtatgag gcgttgggcc ggtcctggcc
     1981 agggccgccg ggttggtcga tcgcacggga catgtggatc accacattcc cctacgcgaa
     2041 actccccgcc gtgtccgatt tcacccgggt cgccgccgcc ctcgaggagc accccctcct
     2101 cgacacgctg gtgcgcggcg ccgcgaccgg gccggcgccg ggccgcactc acgaaccggc
     2161 ggaccctgaa ctcgtcacgg tcctcgatgc cgaccccggc cagatcgctg ccatcgaggc
     2221 ggtccgggcc ggtgagtcga tcgtcatcga cagcccgccc gggacgggca agtcccagac
     2281 catcgtctgt atcgccgctg acctggcccg cagtggtcgg tcgacgctcg tggtggccgc
     2341 cgccgccgat tcgcgctccg ctgtgacggg ccgcctggtc cactgcggcc tcggcagcct
     2401 ggtgcgtgaa cctggcaccg ggtcagcgcc tgcggcccat cggcccagcc agttcgcgcg
     2461 cgcgtcccag gtgcaatcgg cctggacctc ggcggggcgc gaactcagcg aacatgtggc
     2521 gcggatgcat cgcggccggg agccctggtc gctcagtgtc gatgagatcc aggaacgcat
     2581 cgcggccgtc tgcgaggcgc ccgagcctcc gcgatccaag atccggctca gcggcccgca
     2641 cctgggggcg ctcggtccgg cggaggcgga gcgctggatg acgaccctgc gcggcgtcgc
     2701 cgagcgcggg gcgtggccct cgggcgagga gccggccgac ccgtggtggg gtgccgcgat
     2761 ccgcaccgac gaggacgccg agcgcgctcg gctcgcggcg aaggccctgg gcgcgagcgg
     2821 tctggcccaa ttcgacgcgg tgttcaccga ggtattcgcc ggggtcagcg tgccgccgct
     2881 gcgcaccctc ggccagcacg gtgccttcat cgccgagatg gcccggatcg ggcaggtcct
     2941 tgaggtcttc cgtctcgggg tcttcgacgc gcccttggag gagtggaccg cccacacctc
     3001 cggtggcacc ttcgagcggt ggcggcacgg acgcgcggtc aaggcgctct tgcgtccggg
     3061 cgcgccgcac cacgacgtcg accccctgct gcactcggca ctgcaagcgc gcccggtctg
     3121 gcagcaggtc cgcggttcgc agatcatgcc gggacaggta gcggggctcg ggcgggcccg
     3181 cgaggcatac gacaagctcg ccgcgcacgc gagctacctc ggcgagcggt tgcgtggcca
     3241 tcccgacctg ctcgcggagc ggttgccgca gctggctgcc cgggccgcgg acctctccgc
     3301 ccaacgcagc cggctcgagg tcctgccggc cgtcacggac gatctggacg cggcccggac
     3361 tgcggggttg ggcgagctga tcgaagacct cgccgcgcgc cggctgtccc cggagcacgt
     3421 ggccgcagag accgaattcg tttggctcgc atcggtgctc gcgcagacga ccgcatcgga
     3481 cagtgggtat gcccgggtca ccggggacgc tgtccgcgca gccgtcaccc gcttccgcga
     3541 gaacgatcgc gcgctgcaac gagccgcggc agcgggcatc atcgccgaac gcggcgaggg
     3601 caagcccctc atctgcgtct gcagcccgca caccgtcggt ctgtacctgt cccgcaaggc
     3661 gcacttcgac gtcgtgatca tcgacgatgc gcaggcgctc gccaccgccg acgggatcag
     3721 cgcgctcgcc cgcgccgacc aggccgtcgt cgtcggcgac tcgcacctgc ccgggccgac
     3781 cttcttcacg gcaaccgccg gcggcgcggc ggccaagggc tcgccctctt cgtcgttgct
     3841 gaccgaggcg gcggcggtgt ggccggtgca ccgctttgcg tggcactacc gcagccggga
     3901 ccagcgcctg accgagtcgc cggtcgccac ggcatacccc gaactgcgga cctttccctc
     3961 gccctccgac accggggtgg tccgggtcga ggccgctccg gtcaccgagc acgaccacgg
     4021 cctcgtcgcg caggcggtgc gcgccgtcgt gacgcacgcg cgtctgcagg cgaaggagtc
     4081 gctggccgtc ctgaccgtcg atcccgaact cgcgcaacgc attcgggacg ggatccgcgc
     4141 aacgaccgac ccgagtgtcg ccgacttcct cggcgagcaa ggcggagaag ccctggtcgt
     4201 cctcgacgcg gcccacgcca ccggtgtcgt gcgggacgcg gcgatcgtgg tcctcggaca
     4261 gcagaccccc gacctggtcg ccggcgccgc cggcgcccga ctgctcaccg cggcactgac
     4321 gcgggcccgg gctcgccaga ccgtgctgca taccctcgac gcggcgaagc tggcggacgc
     4381 ggccggcgac ggtccggctc gcctcggcgc gcggatcctt gccgatctct tggcgaacga
     4441 ccggccgaag gtgaccacga cctcggagtc cacactgacc cgcgaccttg cccgccgact
     4501 gaagagccgt ggtctgcgcg tgcgcgaggg cgtcgccccc gggatggtcg acctcgccgt
     4561 gcgcgacccc ttcacccgtg gcccggtcgc gctggccatc caaatcgacg gacccgcgta
     4621 tgccgctctc gggggcaacc ggatgcgcga tcgccttgtg caagaacagc ttcggcgcct
     4681 cggctggcgc ccgctacgca tcctgaccgt cgacctgttc cgagacccgg cgcgcgagga
     4741 ggcccgcatc gtggcggcga tggagcggat ggcggccggt gacatgcgca ccggaccgat
     4801 caccggcaaa ggggcccggc gcgccagccg cccggcgacg ggaagggccg ctgacggtgc
     4861 cgcagaccaa cccgccgcaa ccggacgccc cgagcagacg cgcgacgaca ccgacgccgg
     4921 ctggggcgag cgccccgcct ccggaggcga cccggcgcac gaccgttggt tgcaggagaa
     4981 ccgccccccg cactgggagt gacgctgcac cggatgtggc gggtggcacg aattgccatc
     5041 tcgatggggc ggaatcgaca tctcgacggg ggtgggtcgg ggcacggcga agggcgggtg
     5101 gaagggaggc ttccacccgc ccttcggcgg ttatcggtgc ctagcgcggt aggcggggtg
     5161 agtcagcggc gggtcagcgc gtcgcggatc tcggtgagca gggagatctc gtcggagacc
     5221 tgctcgtcct cggcgaggaa gcggtcgcgg gcggcctcgt agggcttgac gacgaagaag
     5281 tagagcatga acccgatgat caggaaggcg acgagggcgg tgaggaacgg ccccaccgag
     5341 gtccaaccgc ccggcttcca gtcgtcgaag ttcggggccc cgccggcctt ggccaggcct
     5401 tcgatgatga tctcggtgaa cgccgtcacg accgcggcga acgccgtgcc catgacgaac
     5461 gcgacggcaa gctcgatgag gttgccgcgc aggataaagt ccttgaaacc cttcatggga
     5521 ttctctgtgt cctttactta gggagtctgg gcgccgtcac acacattggg aggcaccggg
     5581 cccggaaacg ggccaccgaa gaggatagtg gaaatccgga gtaatgtccc gtcggcccgg
     5641 cccccacggg cgcgcgacgc ggccgcccta agggattctg cgcgcttggt ccgaggcccg
     5701 gctcgccagg acgacgagca tcggcgatcc ggcgctggag gcgcgctggc ctgcggagac
     5761 gtgctcgaca tcggcgctgt cgatggcgag gaagacactc gcctcgccac cgggggacgc
     5821 gccggtcgcg ggatcggtgc cggacccctc gcccgggcgt gtcacgtcga cgacctcggc
     5881 gtcacgggcc atcggtttgc gggcgccagc ggcatacaga tcgaccgtgt ctccgggccg
     5941 cagccgcacc gcgagcgcgg cgggcaccgg caggaccacc ccgacccggt cttcgggcag
     6001 cgcggtagcg cccagcgccg aggtgcgtgg ggtggccggg gcggtgggca ccgggtcgtc
     6061 gccgccgccc ggcaggtcga ggccgggtat gccgacccca cccgcctcct cggtcgcgct
     6121 cgccgagggc gtgggcgcgg ccgccgggac cccgtccgcc cccgggcgca ggatgagcag
     6181 cgcaagcccg gcggcgagga tcaacagggc ggcgatccgg cgcagcaggg tccggcgcca
     6241 ggccgcccgg cgtccggccc cgatgaccgg ggcgaacggg gagtcgggcg ccatcagctt
     6301 gcccttggtc ttggtcggtg agagcacgat ggcctccgtc tagggcagct cgaaaggcag
     6361 cttcaggccg tcgagggcgc gccggcacgg gcaggtggcg ctctcgtcgg gttcgtgcgg
     6421 tggcagggtg gcgaccgccg accgcaagac gtccttgagg gtctgcatgt gttgcgcgaa
     6481 cacggtgagc acgtcggcat ggttgaccgc cggcaccccg tcgatgcccg cgtcgtgatc
     6541 cgttacgagc gccaccgtcg tgaagcacat cgccagctcg cgggccagcg ccgcctccgg
     6601 catggccgtc atgcccacga tggaccaacc ctgggcctgg tgcatccggg actccgcggc
     6661 gctggagaac cgcggacccc ctacgacgac cagcgtgccg ccgtccgtgg cggacaggcg
     6721 tccgtcctcg cgcaccgcgg acaacacggc agcgcgcccc cgctcgcagt aggggtgggc
     6781 gaaactgacg tggacgacag gtcccggctc gtcatagacc gtgtgggccc gccccgaggt
     6841 ccgatcgacc acctggtcgg gcacgacgag cgatcccggc ccgagctcgg gccgcaacga
     6901 tccgaccgcg cacggcgaaa gcacctgccg caccccgagg ctgcgcagcg cccagagatt
     6961 ggcgcggtag ttgacccggt ggggggcgaa ctggtggtgg gcgccgtggc gggcgacgaa
     7021 tgcgacccgg cgcccgccga ggtccgcgat cgcgatctcg tcgctcggcg ccccgaacgg
     7081 cgtctcgacc cgatggtgcg gggcgccttc gaagaaggag tagaacccgg agccgccgat
     7141 gaccccgatc tccgcctgct ggtcgtttgc cgtcatgccg cgaccttacg gccgcgcgca
     7201 catccgggga agcggggcct aggagcctgt ggataccgcc gaggtggact tcgcgggtgt
     7261 ggacgacgtg ctcgaggagg acgcgctcga cgacgatgag gagtcaccgc tgccggacga
     7321 gccgcccgaa gaggagttgg aagaggagcc ggaggacgac ccgccggagg agggcacgga
     7381 actggtgctg ctcgagcggg agtcggtgcg gtagaacccg ctgcccttga acaccacgcc
     7441 cacggcgttg aactgtttgc gcagccggcc ctcacactcg gggcacacgg tcagcgagtc
     7501 gtcgctgaag gactggacga tgtcgaaggc gtggccgcaa tcggcgcagg cataggcata
     7561 cgtgggcaac gaagtcctcc gaaagaccag gcgccgaacc cgtcgcggac ccggcggcat
     7621 tgactacctc atgataaggc gagcccgtgc cacggcattc ccaccgctca accgaaggtc
     7681 gccgggcgcc cggggcgctt gcgcaccttg agccggtctt ttttcgtcca ctccgccagc
     7741 cggccgccct cactaatcgc gcgcatgagc tcctcgaccc gggagcgttg ggcgtcggtc
     7801 gtcacgacga ggagctggtc gccgtggtgg atcttggtcc gcggggtcgg gacgatgttg
     7861 tgcccgtcgc gcaggatcag cgtcacatcg gcgccgaccg gcagccgcag ctcgaagacg
     7921 gccaccccgt gcagcatcga cgtcggcccg acatcgacgg tcagcaggga cgcgttcagt
     7981 tcctccaccg gcgtggactc caccgtcagc tgccgggtgc ggtgcgcctc ggtcacgccg
     8041 agccggcgcg ccacccacgg cagcgtcggc gcctggagca acgtgaagat gacgacgagc
     8101 acgaagacca ggtcgaagat ccagtcgaca ttgggtgccc gctcgatcac cggcaccgtc
     8161 gcgagcacga ccggcacggc cccacgcaag cccgcccacg acaagaacgc ctgttgccgc
     8221 aggctgaacc cgaaccacga cactgatgcc gcgaccgaca gcgggcgggc gaggagcagc
     8281 agcacgagac cgacgaccag cgccggcacc agctgttcgg cgaaccccgc gggggaggcg
     8341 agcagaccca gcagcacgaa cagcccgatc tgcgccagcc accccatggc ggtggcgaac
     8401 gcgtgcaccg ccggctggtg cggcagcccg aggttgccga gcacgagcgc ggcgagatac
     8461 gtggccagga agcccgacgc gtggagggac gcggccgcgg catacgccag cacggccacg
     8521 gcgatcacgc cgatcgagaa caggcccgaa ctcccggccg cgctgcggcg catcagctgc
     8581 cccccggcat acccgaccac cagcccgatc accgcaccac cggccaactc ggtcgccgcc
     8641 agcccgagga tcgcccacgg actgtgtgcc tcgccgggat tggcggcggc gagggcgagt
     8701 gcgctcacga gcaggacaac gggggcgtcg ttgaatccgg actcggcttc gagcatcccg
     8761 gtgagccgtc gcggcaaggg caccgatcgg agcacggaga agaccgccgc cgcgtcggtc
     8821 gaggagacga tcgccgcgac gagcagggcg gcgttccagg accagccgag gatgaagtgc
     8881 gccgccacgg cgaccacggc cacggacacc ccgacgccca gcgtcgccag caggccggcg
     8941 ggggcgatgg agtccttgat gccatcccag cgggtcgtga ggccaccctc gatgagaatg
     9001 aggacgagcg cggcataccc gagcaccagg gtgagctgct cgtcctcgaa gcggatgccc
     9061 agaccgtttt cgccgatcag caggccgatg cccagataga tcagcagcga cggcagcccg
     9121 gtccggttgg acagccggat ggccacgacg cacacgatca gcgtcagcga accgaccagc
     9181 agcgccgtcg tcaactcgcc cagggtgaag cccgtttggg cgtcgccggc gagggggagc
     9241 atcggtgcct ctctctcgga gtctgggcgg tggcccccat cctgtcaggg tcggtgtcgc
     9301 tgaccgtgtc tcgtccacgc cggcccgtcc gcggtcacca cggcatcgac cggctgatcc
     9361 cagtggtccg cgggcagcgt ggcgtcgagg aactcacccg gatgcagcag cgtgacgacc
     9421 accgcgtccg gccgggcgcg cgggagggtc cggtcgtagc agccgccggc ctgccccatc
     9481 cgggttccgt ggcggtcgac ggagaggccg ggtatgagga gcagccggca ggcggcgacg
     9541 gcgtcgagac ccagcggggt ccggtccgga tcgtccagct cgtgccagtc cagatcgaag
     9601 ctcgccaggg tgatcggcac gatcacgtgc acagcccgct gggtgagggc gcggcatacc
     9661 gcctgcgtct ggggttcggc gggcagggct tcgtatgccg ttaccacctc cccggggccg
     9721 atcgccaggt tgtcggcgag ggcgagggta tgccgcgcga ttccctcccc gtccgccgcc
     9781 aggtcgcggg tcgcagcgat ggcccggcgc gccgcgcgcc gctgggcgcg cacctgttgt
     9841 ttcacgctca gccccatgat ggccagcgta ggcgcgtccg gataccggcc catgcattac
     9901 taggctggcg ggtatgagtg atgccggact cgcgcacgcg accaccgcca tggccgagcg
     9961 aggcgcctcg ccggcggcga tcgcgacctt cgaccgcttc tatcggatgc tcgagtcggg
    10021 ggagcagggg ctgatccccg aatccacgat cgcgcccctg accgatgtca ccaccctcga
    10081 gggggtcgcg gccagcgagg cggaccgccg cgcggcgctc gcgaaggtcg tcgtgatcaa
    10141 gctcaacggt gggctcggca cgagcatggg cctgtcgggg ccgaagacgg cgctgaaggt
    10201 gcgcggggag ctgaccttcc tcgacatcat cgcccagcag acgctcgcgc tgcggcagcg
    10261 gtatgccgtc gacctgcccc tgctgctgat gaactccttc cgcacgcgag aggagtcgct
    10321 ggagatcctg gcggcctatc ccgacctgcc ggtggcgggg ttgccgctcg acttcatcca
    10381 gaacgccgag cccaagctga ccgccgacac gctcgagccg gtctcgtggc cggccgaccc
    10441 ggagctggag tggtgcccgc ccggacatgg tgacgtctat gtctcgctgc aggggaccgg
    10501 cctgctgtat gcactgcggc agagggggat ccgctacgcc ttcttgtcca atgcggacaa
    10561 cctcggggcg acgtgcgacc ccgacatcgc ggcctggctg ctggcgaacg acatccccta
    10621 cgccgccgag gtgtgcgagc gcacggccaa cgaccgcaag ggcggccacc tcgccgtccg
    10681 cacccgcgac aaccaactga tcctgcgcga cagcgcgatg gtcgtcccgg gagaggacga
    10741 gcacttccag gacaacaccc gccacaccct cttccacgcc aacaacctct gggtcgacct
    10801 cgacgagctg gccgcgctga tggagcgctc cggggggctg gtgccgctgc cggtcatcgt
    10861 caaccgcaag accgtcgacc cgaccgaccc ctcctcgact ccggtgatcc agatcgagtc
    10921 ggccatgggc gcggcgatcg aggtcttccc cggttcgcag gcgatcgcgg tgccgcgcaa
    10981 gcggttccgc cccgtcaagt cgaccaacga gctgctgctg gtgcagtccg acatcttcga
    11041 cctcgacgcc gacgccaacc tcgtctcgcg catcacccac tcggagccgg ccatctcgct
    11101 gggcaagccc tacaagttcg tgcccgacta tctcgcgcgg ttcccccagg gtgtgcccgg
    11161 gatgcgtgag tgcaccagcc tgaccgtcga cgcggacgcg accttcggcg cgcacgtgac
    11221 ctgcgtcggc gatgtgacga ttagcgggga gccgcgcgtc atccccgacg gcgccacgct
    11281 gaccggaacc ctatgaggcg caatgcgatc ggtctctgag caccgcgaag cgatcctcgc
    11341 cgcgctgccg ccccgcgccg gtgccgaggt gccgctggcg gattgcctcg gcctgatcac
    11401 gacggcggac gtcgtggccc gcgtcgcgct gccgggtttc gacaattcgg cgatggacgg
    11461 gtatgccgtg cgcgccgccg atgtcgccgg cgcctccccc gaggggccgg ccgccctcac
    11521 cgtggtcggg gaagtggcgg caggcgccga tgccgcgtcc gtgacggtcg gtgcgggaca
    11581 ggcggtccgg atcatgaccg gggccatgct gccggcgggc gcggacgccg tcgtcatggt
    11641 cgaggccacc gacggcggcc tcgacgtggt gcaggtgtat gccgccgtcc cccacggccg
    11701 ctccatccgc accgcgggcg aggacgtggc gcagggtgcg ctggtcatcc cggcagggac
    11761 gcgcgtcaac gcccggacgt tggcgttggc cgccgcgacc ggccacccca ccctgcccgt
    11821 ccggccgcgc ccgcgcgtgg cgatcgtctc caccggcgac gaactcatcc cgcccggaga
    11881 accgttgtct cccgggcaga tccacgagtc gaactcggtg atgctggcgg cggcctgccg
    11941 cagccgcggc gccgaggtcg tcgcgaccgt cgccgccggg gacgagcccg aggcgttcgt
    12001 cggcctgatc gaggatctcg cgtcggagtg cgacgccatc gtcagcagtg gcggcgtgag
    12061 catgggggcc tacgacgtcg tcaaggcggc gttgcgcgag cacggcatcg agttcgtgca
    12121 ggtggcgatg cagccgggca aaccgcaggg gttcggcctc ttcggggagc accgggtgcc
    12181 ggtgttcgcg ctgcccggca atccggtttc gtcgttcgtg tccttcgagg tgttcgtcgc
    12241 gccggccctg gaggcgatga tgggcggcac gcacgagcgg cgcgtcgtca ccggtgtcat
    12301 gggccacgca ctcacctcac ctcgcggcaa gacacagatc gcgagggcgg tgacgacccc
    12361 cgaaggcggt gcgtgggttg tcaatcctgt tgtgggacaa gggtcgcact tcgttgccga
    12421 tctggtgcgg gccaacagcc tcgtgatcgt gcccgcagac gccgagtcgc tcgcggccgg
    12481 tgacgaggtc gaggtctggc tgctctccga ccccgggccg atcccatgaa cgagctgcgc
    12541 ctgacccacg tccgcgccga cggcagcgcc cacatggtcg atgtctcggc caaggccgtg
    12601 acggcgcgcg aggcctcggc tgccggccga gtgttgctgt cggagaaggc ggttgctgcg
    12661 ttgcgtgatg gcgcggtcgc caagggagat gccctcgccg tggcccggat cgccggcatc
    12721 caggccgtga aacggacccc tgacctgatc cccctcgccc atccgatcgc ggtgcatgcc
    12781 gtcgaggtcg acctcacggt cgctgacgac ggcgtggaca tccgggcgag cgtccggacc
    12841 gctgatcgca ccggcatcga gatggaggcg ctgacggccg tcgccgtggc ggcgttgacg
    12901 gtgatcgata tggtcaaggc catcgacaag catggccgga tcaccgacat ccgggtcacc
    12961 gccaagtccg gcggccggtc cggcggttgg gccgaaaccc ccaccgagac aacacgaact
    13021 cgtccgcagc cgagccagga gatgccgtga ccgacaccgc tcaggatcgc ccgccgccgc
    13081 tccggggcgt ccgggccgtc gcgatcacgg cctcgacccg ggcctacagc ggcgtctacc
    13141 ccgaccgggg cgggcccatc atcgccgaga ccctgcagtc ctggggcgca ctggtcgcac
    13201 cgaccgtcgt cgtgcccgac ggcgacgcgg tggcgacggc gctgcgggag gccgtcacgg
    13261 catacgacct ggtgatcacc accggcggca ccggctgcgc gccgaccgat ctcaccccgg
    13321 aagccacccg ctccgtcctg actcgggaga tccccggcat cgccgaggcc atccgggctg
    13381 acggcctcgc gaacggcgtt gcgggagcga tgctttcgcg tgccaccgcc gggctcgccg
    13441 gacggtgcat cgtcgtcaac ctgcccgggt ccaccggtgg ggtgcgggac ggattgcgcg
    13501 tgatcgcacc gatcctgggg cacctcgtcg agcagttgcg cgggggcgat cactaggtgc
    13561 gctcgcggct gtggccggtg gacctcgcgg ccacgctggc ggatggcgga cagctgggcc
    13621 tgcggcccct gggccgcaag gaccgcgagg actatgtgct cttgcgcgcg gccaacaccg
    13681 actggctgcg gccgtgggat ccgacgagcc ccgacggcat accgcgcacg gtctccttca
    13741 ccagcctgct gagcgagcag aaggccgcgg cccgcgccgg gtcgctcctg ccgttcgccc
    13801 tcaccgtgga cggagccctc gcggggcagc tcaacgtcag caatatcgtc cgcggcgcct
    13861 tccggtcgtg cacgatgggc tactgggtgg ggcgcgagtt cgccggccgg ggcatcgcgc
    13921 cgctggcggt cgccgtcgcc ggtgactacg cgatcgccgc cgccggcctg caccggatcg
    13981 agatcaacat ccggccggag aacgtcgcct ccctggcggt ggtgcacaag ctggggtttc
    14041 gctccgagtg catccggccg cgctatctgc acatcgacgg agcctggtgc gaccatctct
    14101 ccttcgcgct gacgaccgag gagatcggcc cgggcggcct gcgcgcgcgg ctcgccgggc
    14161 tcgagtccgg acgaccgcgc gacgccgtgg ccatccgccc ggccaccgcg gaggacctgc
    14221 cgtggctgac ctgccagtgg cggcgggcgt gggggagcac ccaggtcgcg atcggcgatg
    14281 atctgctcga cctgcgggac cacccggcac gcgtcgccgc cgctgcgggc gagctgctcg
    14341 gttatctcgt ctaccgggac ctgggcggcg cacgcggaat ccttgcgctg gaagcagttc
    14401 accccggacg cggggtgggt caggccctca tcgaggacgc cgccgaagac acccggctgc
    14461 ccctggtcct gaccaccacc aacgacaatc tgaacgccct cggcgtctac caacacctcg
    14521 ggttcgtcat cactgacgtg cggccaggag ccatcgaccg ggcgcgcgag cgcaagccga
    14581 cgatccccct gctcggcgac aacgggctcc cgatccggga cgagatcgtg ctgaccctgc
    14641 cgcaatccga ctgatcgaca gacgaattcg gcgagactgc acagccgaca caccggccac
    14701 cctcccgcgc tccggcgcgc aatcttcata cggttgtcac cgtgcagccg agcagcctga
    14761 tcttccttgt cgtcattgcc atttgggctg cctacctgct gcagcactgg ctcggccggc
    14821 gcgaggacct ggcgaccgcc cggtcggtcg atcgcttctc ggagtccatg cgcatcctcg
    14881 accgtgctgc gcgcgaccag cgcgccgaac ccgcccgggc cgaggtctcc tccactcggc
    14941 tgcacccgcg gcaggtgacc gtcacgcgtg ccgcagccct cctggccgcg accccggccg
    15001 ccgccactgc caagaccgcg gcccgcacgt tcggcgccac ggcggtctcg gggctcgccg
    15061 gccgccgtcc cgcggccacc gggcggcgcc gcgccggtcg cgcgacccga ggtcttttcg
    15121 ttctgggcgc tggtgcactc accctcgtca cgctcgtgct tgcgacgatg agtgtcgtcg
    15181 gctggtgggt cccgatcctt gccctggtcg tgaccgcggg cgcccagtgg tggctgcgcc
    15241 gcgcggtcgt ggccgaacgc gccgcccggc gcgcggcccc ggcccgcccg acgatgcagc
    15301 gccccgcaac cacgacccag cggccggtcc agcgtcgggc cgcgcagcct ccggcacggg
    15361 tcgcccaggc aaccgccggc gggcgtgtcg agaccgtcgg cgatgacacc gccgatgccc
    15421 ccgcgacggt ccaagagacc ccggctgccc gcgagaccct ctacgacctg gcggcgatcg
    15481 aagaggccga ggccgagtcc gcccgggcgc gggccgcggc ggaagggtgg aacccggtgc
    15541 cggtgccgcc cccgacctac acgctgaagg ccaaggccgt cccggccgag gagatcctcg
    15601 gagacgagga cgacgatgtg gccagcccgt tcagcgggga tgtcaccgac ctgagcgagt
    15661 acgccgagcg cgcccgcctg tcgggctgag gctcactcgc tcccgagcgc gtcgtcggcg
    15721 gcggcctgcc gagcccgggc atccgctgcg gcgacctcgc cgtcgacctg ggctcgctcg
    15781 cccgcgggca gcagatcttc ggccttgtcc ggagccggtt cgtcgtccgc gaggaggtga
    15841 tgcccgacgg cgttgacgac agccgccacg ggcacggcga tcagggcgcc gacgatgccc
    15901 cagacgatga cgccggccgc gatggacaag atcacggcga gcgggtggac gcgcacggcc
    15961 cggccgagca ggaagggctg cagcacgtgg ctctcgatct gctgcacggc gatgacgatg
    16021 ccgagcatga tcagggcttg gaccggcccc agcgcgacga gtgccagcag cacggcgacg
    16081 atgccgctca tgagggcacc gacgacgggg acgaaggctc cgaggaagac cagcagcgcg
    16141 atgccgctgg cgaagggcac gcgcaggatg gcggcgccga tgccgatgcc ggtcgcgtcg
    16201 accgcggcga cgaggatggt ggccttgcag aaggccttca gctgccccca ggcgatctcg
    16261 ccgctcgagg cgacgcgggc gcgagccgag cgcgggaaga gccggaccac ccacgcccag
    16321 atgtgcgggc cttcgagcag gaagaagaag agcgcgaaca tcgagatgaa gaagcctgcg
    16381 acgacgtgcg tggccgtcag gccgaacttg gccgccgtcc cgccgaggtc gctgctgctg
    16441 ttgctgagcg cctcgcgggc cttctggaag tactcgctga actggctgtc ggagatattg
    16501 aaggtgtcgc ggatccaggt gcggatctgg tcgattccct caccgacctg gtcggtcatg
    16561 tccggcagct gctcggcgaa ctggctgccg acgaaggtca tcagcccgga aagcaccgcc
    16621 agggtgccga gcacggtgat ggccgcggcg agcccgcgtg gcagatgctg gtcgaggcgc
    16681 cagaagagcg ggtggagcag ggccgcgagc agcagggcga cgatgatggg cacgacgatc
    16741 tccgacaagt gcccgatgag gaagccgaac agggccagtg ccgccgcgat gagcaggaag
    16801 cgccacgccc attcgctggc atcgcgcacc cccttgggca cggcgcttcc gggttccgta
    16861 tgcggctcgc tcacgcccat accgtaggcc ctcgccccac ccgctgcccc gcctgtatgc
    16921 ggcccgctgc cgcgaccgtg cccgattcgg tatgccgcct ggcccggtgt taaggtttcc
    16981 tacgcatctg gggctgtggc gcagttggta gcgcgtctcg ttcgcaatga gaaggccagg
    17041 ggttcgaatc ccctcagctc caccccacgc acaaggctcg aacccttgcc aacggaaacg
    17101 ttggtcgagg ttcgggcctt gttcatgtct gcggcccagg tcagggcgtt ggcgttgacc
    17161 tgcggatcga ggagcacgcg gacacgatca gcgcgaggac gctgaggatc aggccgatgg
    17221 tcatcaccgc gcggacgatg gtacgcagtt ggttgatccc cggcaggccg ttggtgttgg
    17281 ggtcgatgtt gatgtccatc ggca
//
LOCUS       DAOPJG010000053        17190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3511_length_17190_cov_7.932536, whole genome shotgun sequence.
ACCESSION   DAOPJG010000053 DAOPJG010000000
VERSION     DAOPJG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17190
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3511_length_17190_cov_7.932536"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PKU75_06880"
     CDS             complement(<1..460)
                     /locus_tag="PKU75_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80636.1"
                     /translation="MTIAPKAPRKLSMKDRYSALTRDLDWEPSYVSPDELFPQTKFEG
                     IIIHDWSKWEDPFRLTVDAYTKYQAEKDKRLYAVLDGFAQSQGHLSLTDASYLNAMKL
                     FIQGIAPNEYNAHRNFAMLARHLNGGGPRFAALCQCLDEIRHAQTEIHTLA"
     gene            complement(575..868)
                     /locus_tag="PKU75_06885"
     CDS             complement(575..868)
                     /locus_tag="PKU75_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HPF80637.1"
                     /translation="MTTIPAAERPVGVDIQENEQNRAVIEAIEADNPEATVRHMPGMV
                     RVQSPGKLVINRESVEERIGREWETHEFQLAIISYFGHIKEWDDDQILIAWDH"
     gene            complement(872..1888)
                     /locus_tag="PKU75_06890"
     CDS             complement(872..1888)
                     /locus_tag="PKU75_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80638.1"
                     /translation="MQIELRTQVIEPQRKTFTHISDRYGDRPSTRYEEGTIDLQSTVN
                     YHYKPLWAPDKDIYSPDFSELKLADPYSFMDPRQYYYAPYVTARADLHEAFGSTLGYL
                     EKRGLFERLPEAWGGVLQNVVLPLRHYEAAAQMISAYAGRYGWGTSITQCASYASFDR
                     IGNAQILSRTGISYGGGTAEPLALAKTAWLEDDAWQPMRKHVEQMMVEQDWGKSLVAL
                     DVTDRIIYQLLFVHLDDAAITSGAGSYSLVAQHLSSWFADQRKWLDALYKAWVADPEH
                     GAANAATIAAVVDTTIATVREAMAPVAAAADSLIDAGTTDALSAAADQARAAYVTLGA
                     IKEN"
     gene            complement(1926..2363)
                     /locus_tag="PKU75_06895"
     CDS             complement(1926..2363)
                     /locus_tag="PKU75_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80639.1"
                     /translation="MTPFMLRVAEYVGADEDEIDHIVAPMPATRISQKIAASHGADKQ
                     VAIRSLAEQLVCEANAVLGHASDHLTLTDETLPDELAFEVGFRGRAARVSTTFADGTA
                     YGRLVGDGFESELPQELNSADDVPDLLMRLIIESGTPHHTVNG"
     gene            complement(2360..2827)
                     /locus_tag="PKU75_06900"
     CDS             complement(2360..2827)
                     /locus_tag="PKU75_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HPF80640.1"
                     /translation="MNDTPLIVNRPQLTYAAARLALDAAVAEAERIGGAFNVAVADTG
                     GVLLAFARMDGAFAESGAIARDKAWTVASFAGLPSRALYEAIEAEPAVRDGIGGRDRV
                     AAFGGGVPIRIDGALAGAVGASGGSAAQDEQVAQAGADAVIAAYESVAKEGRS"
     gene            complement(2963..3880)
                     /locus_tag="PKU75_06905"
     CDS             complement(2963..3880)
                     /locus_tag="PKU75_06905"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HPF80641.1"
                     /translation="MAVMRMGYVHARVTDLAEAKEHYANTLGLYETHSEPGKVFYKGW
                     DEWDHHSVVLEEGGVGVAKFGFKVEYESDIDLIEKKAQDFGLTVERMSAGENLEVSDG
                     IRFTSPSEHVFEVYHSQKVIGTEVGTHNPDAFPRHLVGVGVPALDHALILAEDVRKME
                     DMLKAVFDFFPTERVQTDLNDDDAHFIASWMTCNNQIHQIAVLEGEQSKLHHFAFKLD
                     NWDQVGRAADLFSMDDVPIDIGPTRHGITRGQTIYFFDPAGNRNEVFAGGYPAYRDRP
                     INKWTPDAIAKGIFYHARELNERFTTVYT"
     gene            complement(3885..4277)
                     /locus_tag="PKU75_06910"
     CDS             complement(3885..4277)
                     /locus_tag="PKU75_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPF80642.1"
                     /translation="MPTVTIEPSGEVIYLEPDETVLSGLYKAGYAYTIGCRRGGCAIC
                     KVDCRAGEFHYNRPIADTVINETERGDGTCLSCRAVPDGDITIEMRGENVRLVNPFLR
                     QINEKARLRAEAKQAADAERLTSTTTKE"
     gene            4668..4850
                     /locus_tag="PKU75_06915"
     CDS             4668..4850
                     /locus_tag="PKU75_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HPF80643.1"
                     /translation="MSTEKRSLPDEETKRKFREALEKKQAGNKVDVSDHSEHGKVDHA
                     SGAEGGQRMFRRKAGG"
     gene            4919..5866
                     /locus_tag="PKU75_06920"
     CDS             4919..5866
                     /locus_tag="PKU75_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPF80644.1"
                     /translation="MAPRVIHTAQALVDEVVEVPGLPERGGNVMAGAHYLYAGGAVNI
                     LIAAARSGAACVHAGALGTGPNADLVRSALAAEGIAWSAPPVADLDTGVCFVMIEPSA
                     ERTFVTTQSAERRISVESLQTSDPQPGDIVCVSGFSLVGMTRDPLVAWLHGLHDDVVV
                     VLDPGAAFAELDFALRTEVLAMTDVWTSNAAEALDVTGLPGMAESVVAVAALLRPEGV
                     AIVRDGPKGCAVHVGGVTTELPGYPQTPVDTNGAGDTHTGTLTACRAAGMEWLEAARR
                     ANVAGAIKVTRRGPATAPTADEIDAFLREHDPTPRRVNT"
     assembly_gap    5962..6061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6062..7537
                     /locus_tag="PKU75_06925"
     CDS             6062..7537
                     /locus_tag="PKU75_06925"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF80645.1"
                     /translation="MGAACHLTRELPGTSYAILEMRERLGGTWDLFRYPGVRSDSDMY
                     TLGYGFRPWRGRQALADGPSILRYIEDTAGEYDVLPRIRYGHKVVRADWSSGSARWTV
                     TSEGRDATGEPRTATATCRFLYCCTGYYDYTEGYTPHFPGIGSYTGQVVHPQFWPEGL
                     PYAGTRVVVIGSGATAITLVPAMAAEAAHVTMLQRTPTYVLNMPSADPVADLVQGRLP
                     QRAAYLINRWNHLLQSWGLYRAAQIAPGAVRKLIRTDAKSHLPQDFPIDVHFNPPYDP
                     WDQRLCLVPDADLYRAIRMGRAEVVTDTIDTFTPTGIRLTSGRELRADIVVTATGLKV
                     LPGGGATLSIDGADVDLRDTVSYKGIMLSGIPNFALALGYTNASWTLKVDLVSRYICR
                     LLAYMERHGFDEVRPLAPPATEARRPIIDFAAGYVQRADPIMPRQGTRGPWRLPQNYL
                     SDRARLRRRGRLDDGVVFTRRADRVAVKADVDDKRDLPWAP"
     gene            7534..8001
                     /locus_tag="PKU75_06930"
     CDS             7534..8001
                     /locus_tag="PKU75_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF80646.1"
                     /translation="MTSDEKAKTPETDEFGGLIGVGSARTIRAPWHPSSITRIRAAVI
                     ADLQARDVEGETLDEVAVVTSELVTNALRHATPLHDNTVRVHWTERNGIVEVEVTDGG
                     GETEPRARPEAVFAVSGRGLRIVRSYAHEWGVSEKKGAVTVWAALGGPSRRRA"
     gene            8010..8885
                     /locus_tag="PKU75_06935"
     CDS             8010..8885
                     /locus_tag="PKU75_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5926 family protein"
                     /protein_id="HPF80647.1"
                     /translation="MGKASRKKKDTAAPRAPRPAAYNARPFRDLPGESQWVAMAELLP
                     AATVTVPLKAGAVEGGAREVTIVTVLPMAWPALHRADGTLLVATQGGGQSGDKSRDLA
                     SAILAAAAAEPGTPLPQVPMATDATPALQDLLDLTSGPEVMVHEGFDFWVEGQELDAA
                     GQESLARANDSVLPTAKVDGADAAYWCLIGDRPHVRAVLAEDEDEATNALARLWAAGT
                     AALGDGTRLLGAFRAAGLLIPVWELADGTDADGVAAPLAEWKARYAAALTDAPLTPEE
                     RRAKAGILNRQVTLR"
     gene            8891..9622
                     /locus_tag="PKU75_06940"
     CDS             8891..9622
                     /locus_tag="PKU75_06940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF80648.1"
                     /translation="MPPTTPAPAPGPTTAPTRVAAIIPAKSEADRIVATVTATAGLPH
                     VDLVVVVDDGSTDATARLAEEAGAVVVRHTANQGKAAAMMTGAKTVAARDDPAAPRAL
                     LFVDADLRESAANLAPLMTPVLSGAVDMTIANIPRANSSKGGGRVVRLAQRTILGRTG
                     REIAQPLNGMRCLTRDAFRAALPLAPGWGVEVGLTLDVLQAGLRVVEVPVDFTHRATG
                     KDWRGALHRGRQLRDVARVVARRGR"
     gene            9813..11150
                     /locus_tag="PKU75_06945"
     CDS             9813..11150
                     /locus_tag="PKU75_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80649.1"
                     /translation="MPWTLGPAAVTGMLAAAYLLGAAGLWLGLRDRGGWPGWAIAAVA
                     AGALLAMPFGSADHTNYAAYGRIALQGGDPYVESPIAWAGGDDPVTSGVQPPWQETPS
                     IYGPLSTVLQVFAAWAGGDSLRQVVWMWQLLIVGAWLATRWLLRRLFPDASGRVDVLW
                     TMNPFVIGAGVLGAHVDTLATPLMVGALLLARRPGRPWALAAGTLGGLALSVKVTAGV
                     ILVAIALGWLIERRRAATRVLTLAAGAALAAVPLHLWAGARAYDQLGRSRRSISLATP
                     WRKIYEWFGGQLPDATWRSLLFVLAGLTCLALAWLAWRVLCTQACEVDTPVMLALALS
                     IGYAAGAPYVLPWYDQLVWALLPAVAAAGLLERVWIVRSLILALAYVPGRVVGMTPGV
                     EEVTLIWRRDMAPLAAVLVWIALVVASRSACRPASVTPRRPARSPRQPPAPRR"
     gene            complement(11202..12062)
                     /locus_tag="PKU75_06950"
     CDS             complement(11202..12062)
                     /locus_tag="PKU75_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80650.1"
                     /translation="MTSIDDTSGNEPLGTGELSGATAYSAEPPAGGDNVAREGVDGPA
                     GTPDSDPRAGDATLANDAHERTERALDDLDRQADDETANSATPALGSSNTEVFDDAEG
                     APGTPPPGAAQTPAPSDEQRDERREDRDGGGDPGEAGHDRPGEGGNRLSPEEFAAEHD
                     PANHDIAAGGEFRQAGDWTADDAGGPQVWDAEGTLVTEGENAEQQSTAGAADRRVSQF
                     EEVVDGGYSVGSAAPIHDDAMPLGHPVMAWEDTKTYVTEGHPAYGSAEPHVWFTDDEA
                     AEAAGFRRVD"
     gene            complement(12081..12335)
                     /locus_tag="PKU75_06955"
     CDS             complement(12081..12335)
                     /locus_tag="PKU75_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80651.1"
                     /translation="MSNHISRRDDVNPRDGEREHGDVTFADPTNKKYPIDSEEHVRAA
                     WSYINKQANADKYDADEVETIKGRIKRAAKKYDIEISDDH"
     gene            12460..14376
                     /locus_tag="PKU75_06960"
     CDS             12460..14376
                     /locus_tag="PKU75_06960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPF80652.1"
                     /translation="MPMQPLELPGPHGDWAQFVDDLVECGLADARRIATGLKDGAPRS
                     AHEVLDRWNDSSIAVSTAGGVAGLLAQVHPEVEVRTRSEAALQELAKFGSELSLDKEL
                     YAVFAAVDGGGLDPEAARLLAKVLRDFRRSGVDRDDATRDRLKAIDERLTVLGQDFSR
                     IIRDDVRTIRIAPERLDGLPKDYVDAHPPGDDGLVAITTDYPDLIPFTTFAHDGAARR
                     ELHTAFLNRGWPDNDPVLHEILDLRHEMATLLGYPDWAAYDAEVKMIGTGEAIGEFID
                     RITDLADAPGRRDLAVLLARARQDDPGIEALTAADNAYYSELVRREDYEVDAQEVRRY
                     FDFAKVSAGLLDVTGRLFGVSYRPRDDAPVWHEAVTAYDVLRGDEMIGRIYLDLHPRE
                     GKFKHAAQFGLVSGVAGRDLPEGALVCNFPTGLMEHTDVVTLFHEFGHLVHHVLGGAQ
                     RWVRFSGVATEWDFVEAPSQMLEEWAWDANVLRSFATDADGVAIPGELVAKMVAAHEF
                     GKGRHARTQMFYAATSLGLHRDRPADHTAYVAQLQERYDLLARLPDTHFQAAFGHLDG
                     YGSGYYTYMWSLVIAKDMFSAFDRTNLFDPEVAERYRDRVLAQGGRADAADLVADFLG
                     RPYSFDTFGEWLAR"
     gene            complement(14390..14734)
                     /gene="crcB"
                     /locus_tag="PKU75_06965"
     CDS             complement(14390..14734)
                     /gene="crcB"
                     /locus_tag="PKU75_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HPF80653.1"
                     /translation="MTVLLVAIGGAVGALGRYLTDRVVARVAGRGFPWGTLVVNVAGS
                     FLLGLILGRTDGHLAALLGTGFCGALTTYSTFSSETVGLLDERAGGRAVGNVVLSIAL
                     GLAACVLGAALA"
     gene            complement(14731..15222)
                     /locus_tag="PKU75_06970"
     CDS             complement(14731..15222)
                     /locus_tag="PKU75_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HPF80654.1"
                     /translation="MPSARRRYPSLAGDILAAIAAGGALGAVARYAVASWDGLTRAVD
                     VGWGGVSDAVLIVNAVGSFLMGLLVCFVVRRPAHRLLRPFLGTGFLGGFTTFSTFAYD
                     TRALWTSGESLAAASYLVLTPVTAIGACWLGVQAMHRILGWPLPREDEAGDLPTPAEE
                     GTA"
     gene            complement(15248..16117)
                     /locus_tag="PKU75_06975"
     CDS             complement(15248..16117)
                     /locus_tag="PKU75_06975"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HPF80655.1"
                     /translation="MSAHLVAVLGRGVVGADEPVVTVDDLGFARGDGCFDSARVLTDT
                     DGAARVVNLGEHLDRLDAAAAALDIAGPTRDAWEALVAEALVAWHGSGEAVLKLVLTR
                     GREWAPDAGPLALLSITEKPARPRGRVPRALRAVTLTSGRPSDAFKDAPWLLGGVKTL
                     SYVGNVAAAREAKRRGADDAIFVSTDGFAMDGPTSGLIVVTDGVMRTPPTGDTGILAS
                     VTVDEILAAAAESGMPVRVELVPAGDLLTADGVWLVSSGRGPSPIVEVDGSPIRVDEE
                     LAERVAAMAGFGG"
     gene            complement(16151..16795)
                     /locus_tag="PKU75_06980"
     CDS             complement(16151..16795)
                     /locus_tag="PKU75_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF80656.1"
                     /translation="MTASPDPHRLTLVVDDHPVVRRGLVALLTGESWAGEIREAATVG
                     DALTFIHSASPTFAVVDLKLPDGSGTSVVKALTSYAPGCRSVVLTMDGDARLVRAALD
                     AGATGYLLKDTPPEDLVAALRTVADGGVVLGEQLGVARTDRGMTGGPFRQLSPREVRI
                     ALLVATGATNSQIADRLGISDKTVRNQVSAITTKLDATGRVGIANLARENGLLT"
     gene            complement(16806..>17190)
                     /locus_tag="PKU75_06985"
     CDS             complement(16806..>17190)
                     /locus_tag="PKU75_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF80657.1"
                     /translation="VVLDAPAELAPMPPAVEIAAYRIVTEAVTNVARHAVGVTRCTVS
                     VRAAQHLVLLVGDDGPGVQAGPGAGVGLASMRARAEELGGSCEISGTPEGTTVRVVLP
                     LSASPRAAQPRTSRDVVPVDGPRIG"
BASE COUNT         2494 a         5760 c         6099 g         2737 t
ORIGIN      
        1 tggcgagcgt gtggatctcg gtctgcgcgt ggcggatctc gtccaggcac tggcacaggg
       61 cggcgaaccg cgggccgccg ccgttgaggt ggcgggcgag catcgcgaag ttgcggtgcg
      121 cgttgtactc gttcggcgcg atgccctgga tgaagagctt catcgcgttc aggtatgacg
      181 cgtcggtgag gctcaggtga ccctggctct gcgcgaagcc gtcgagcacg gcatacagcc
      241 gcttgtcctt ctcggcctgg tacttggtgt aggcatcgac ggtgaggcgg aaggggtcct
      301 cccacttgct ccagtcgtgg atgatgatgc cttcgaactt ggtctgcggg aacagctcgt
      361 cggggctgac gtaggacggc tcccagtcca ggtcacgcgt cagcgccgag tagcggtcct
      421 tcatgctcag cttccgcgga gccttcggtg cgatagtcat gaaattgcct ctttctcaag
      481 tcttttcagg aggtcgacac gggtcgttgg actttgtcgg ggtccccgcg acgtaccgca
      541 gcgaggcacg agcgaggaca cttcgtgggg tgactcagtg gtcccaggcg atgaggatct
      601 ggtcgtcgtc ccattccttg atgtggccga agtaggagat gatcgcgagc tggaactcgt
      661 gggtctccca ttcgcggccg atgcgctcct cgaccgactc gcggttgatg acgagtttgc
      721 cgggagactg cacgcggacc atgccgggca tgtgccggac ggtggcctcg gggttgtcgg
      781 cttcgatcgc ttcgatgacc gcccggttct gctcgttctc ctggatgtcg acgcccacgg
      841 ggcgctcggc ggcggggatg gtggtcattg ctcagttctc cttgatggcg ccgagggtga
      901 cgtatgccgc gcgcgcctgg tcggcggctg cggagagggc gtccgtggtg cccgcgtcga
      961 tgagtgagtc ggctgcggcg gcgaccggcg ccatggcttc gcggacggtg gcgatggtcg
     1021 tgtcgacgac ggcggcgatg gtggcggcgt tggctgcgcc gtgttcgggg tcggccaccc
     1081 acgccttgta gagcgcgtcg agccacttgc gctggtcggc gaaccagctg gaaaggtgct
     1141 gcgcgacaag ggagtaggag ccggctccgg aggtgatggc cgcgtcgtcg aggtggacga
     1201 acagcaactg gtagatgatc cggtcggtga cgtctagggc gacgagggac ttgccccaat
     1261 cctgctcgac catcatctgc tcgacgtgtt tgcgcatcgg ctgccacgcg tcgtcctcga
     1321 gccacgccgt cttggccagg gccagcggct cggccgtgcc gccgccgtag gagatgcccg
     1381 tgcgggagag gatctgggcg ttgccgatcc ggtcgaagga tgcataggac gcgcactggg
     1441 tgatggaggt gccccagccg taacggccgg catacgccga aatcatctgg gccgcagcct
     1501 catagtgccg tagcggcaac acgacgtttt gcaggactcc gccccaggcc tcgggcaggc
     1561 gctcgaacag gccgcgcttc tcgaggtagc cgagggtgga tccgaaggcc tcgtgtaggt
     1621 cggcgcgggc ggtgacatag ggcgcgtagt agtactgccg cgggtccatg aaggagtagg
     1681 gatcggccag cttcagttcg ctgaagtccg gggagtagat gtccttgtcc ggagcccaaa
     1741 gcggcttgta gtggtagttg acggtgctct gcaggtcgat cgtgccctct tcgtaacgag
     1801 tggagggtcg gtcgccgtaa cggtcgctga tgtgggtgaa ggtcttgcgc tgcggctcga
     1861 tgacctgggt gcgcagttcg atctgcacgt gaactccttt gtcctgaaat aacttttgct
     1921 tgctatcagc cgtttacggt gtgatggggt gtgcccgatt cgatgatcag gcgcatgagg
     1981 agatcgggca cgtcgtcggc gctgttcagc tcctgcggca gttcgctctc gaagccgtca
     2041 ccgaccagcc ggccgtatgc cgtgccgtcg gcgaaggtcg tcgagacccg tgccgcgcgg
     2101 ccccggaagc cgacctcgaa ggcgagttcg tcgggcagcg tctcgtcggt gagggtgagg
     2161 tggtcgctcg cgtgaccgag gacggcgttg gcctcgcaca cgagctgctc ggcaagcgat
     2221 cgaatcgcga cctgcttgtc ggcgccgtga ctggcggcga tcttctggct gatccgcgtg
     2281 gccggcatgg gcgcgacgat gtggtcgatc tcgtcctcgt cagcaccgac atactcggca
     2341 acgcgcagca tgaacggggt catgaacggc cttctttcgc aacggattcg tatgccgcga
     2401 tcacggcgtc ggcgccggcc tgggcgacct gctcgtcctg cgccgccgag ccacccgacg
     2461 cccccacggc ccccgcgagg gcaccgtcga tccggatcgg gacaccaccg ccgaaggcgg
     2521 cgactcggtc ccggcccccg atcccgtcgc gcacggccgg ctccgcctcg atcgcctcgt
     2581 aaagggctcg cgacggcaag ccggcgaagc tggcgaccgt ccacgccttg tccctggcga
     2641 tggctcccga ctcggcgaag gcgccgtcca tccgggcgaa ggccaggagc accccgcccg
     2701 tgtcggcgac agcgacattg aaggcgccgc cgatgcgctc ggcctcggcg actgcagcat
     2761 ccaatgcgag ccgggccgcg gcatacgtca attgcgggcg gttgacgatg aggggagtgt
     2821 cgttcatgtc gttcctggat cagggcaggc ggcatcgccc cgtggcacaa cagatttgag
     2881 aggtgttgcg gggaggacgg caaggggccg tggccgccct ccccggcgcc tggatccgcc
     2941 cggcgtgagc cgggacggtc gatcaggtgt agacggtggt gaagcgctcg ttcagctcac
     3001 gagcgtgata gaagatgccc ttggcgatgg catcgggggt ccacttgttg atgggccggt
     3061 cgcggtaggc ggggtagcca ccggcgaaga cctcgttgcg gttgcccgcc gggtcgaaga
     3121 agtagatcgt ctggccgcgg gtgatgccgt gacgtgtcgg gccgatgtcg atcggcacgt
     3181 cgtccatgga gaacaggtcc gcggcgcggc cgacctggtc ccagttgtcg agcttgaagg
     3241 cgaagtggtg cagcttggac tgctcgccct cgagaacggc gatctggtgg atctggttgt
     3301 tgcaggtcat ccacgaggcg atgaagtggg cgtcgtcgtc gttgaggtcg gtctggacgc
     3361 gctcggtcgg gaagaagtcg aagacagcct tgagcatgtc ttccatcttg cggacgtcct
     3421 cggcgaggat cagcgcgtgg tccagggcgg gcacgccgac accgacgagg tggcgcggga
     3481 acgcgtccgg gttgtgggtg ccgacctcgg tgccgatgac cttctgcgag tggtagacct
     3541 cgaagacgtg ctcggacggc gaggtgaagc ggatgccgtc ggagacctcg aggttctcgc
     3601 cggcgctcat gcgctcgacg gtcaagccga agtcctgggc cttcttctcg atcaggtcga
     3661 tgtcggattc gtattcgacc ttgaagccga acttcgcgac cccgacgccg ccctcctcga
     3721 ggacgaccga gtggtggtcc cactcgtccc agcccttgta gaaaaccttg cccggctccg
     3781 agtgggtctc gtagagcccg agggtgttgg cgtagtgctc cttggcctcg gccaggtcgg
     3841 tgacccgggc gtgcacgtag cccatgcgca tgacagccat gccgttactc ctttgtggtg
     3901 gtgctggtca gccgctcggc gtccgcggcc tgcttggcct cggcacgcag tcgcgccttc
     3961 tcattgatct ggcgcagaaa gggattgacg aggcgcacgt tctcgccgcg catctcgatc
     4021 gtgatgtccc cgtcgggcac ggcccggcag gacaagcagg tgccgtcccc gcgctccgtc
     4081 tcgttgatga cggtgtcggc gatcggccgg ttgtagtgga actcccccgc gcggcaatcg
     4141 accttgcaga tcgcgcaccc gccgcgccgg cacccgatgg tgtaggcgta tcccgccttg
     4201 tagaggccgc tgagaacggt ctcgtccggc tcgagataga tcacctcacc cgagggctcg
     4261 atcgtgacgg taggcaacct gtgctccctt gcactgcgct tggtggtcag ccgtggtgcg
     4321 gctgctctga gaccgagctt gtcgcgccag ggccccgcgg ctaagggtcg ttcctcagac
     4381 gggaacagcc cccgggggta tgcggccccg gccaccctcg ccccgattcc actgcatacc
     4441 gctgtctaaa ggaatgacta gaaatccata tcgagcagca tcggagcgct actggtcgtg
     4501 ccaggaaagg ggagacacgg tcgtgccgac aaaagggcga cccggccatc tcatacgtgg
     4561 ccgggtcgtg ccagttctac cgttgccagg ggggtcatcg tgggaccgac acgttttcgc
     4621 gaggtaaaca cctgcaggcg aagcggtgtc gatggcagga tctgggcatg agcacggaga
     4681 agcgatcgct gcccgacgag gagacgaagc ggaagttccg ggaggccctg gagaagaagc
     4741 aggccgggaa caaggtggac gtctccgacc acagcgagca cggcaaggtg gatcacgcga
     4801 gcggcgccga gggcggccag cggatgttcc gccgcaaggc gggcggatag ccaggcgctc
     4861 gggtgcaggc ctggccgcat gcgatgtggg gcggccgcaa gtgggaggat gacccctcat
     4921 ggccccccga gtgatccaca ccgcccaggc tctcgtcgac gaagtcgtcg aggtgccggg
     4981 gttgcccgag cggggcggca acgtcatggc cggggcgcat tacctgtatg ccggtggggc
     5041 ggtgaacatt ctcatcgccg ccgcgcgctc gggtgccgcg tgtgtgcacg cgggcgcgtt
     5101 gggcaccgga ccgaacgccg acctggtgcg gtcggcgctg gccgccgagg gcatcgcgtg
     5161 gtcagcgccg ccggtcgcag acctcgacac cggcgtctgt ttcgtgatga tcgaaccctc
     5221 ggccgagcgc accttcgtca ccacccagag cgcggagcgg cggatcagcg tcgagagcct
     5281 gcagacctcc gacccacagc cgggggacat cgtgtgtgtc agcgggttct ccttggtggg
     5341 tatgacgcgt gacccgctgg tcgcgtggct gcacggtctc cacgacgacg tggtcgtcgt
     5401 cctggacccc ggcgccgcct tcgccgaact ggacttcgcc ctgcgcaccg aggtcctcgc
     5461 catgacggac gtctggacct cgaacgctgc cgaggccttg gacgtaacgg ggctgcctgg
     5521 catggccgaa tccgtcgtcg ccgtggccgc cttgctgcgg cccgagggtg tggcgatcgt
     5581 gcgtgacggc ccgaagggct gcgcggtgca cgtcggcggg gtgactacgg agctacccgg
     5641 ctatccgcag acgcccgtgg acacgaatgg cgccggcgac acccatacag ggaccctgac
     5701 cgcctgccgc gccgccggga tggagtggct cgaggccgcc cgtcgcgcca acgtcgccgg
     5761 ggcgatcaag gtgactcgcc gcggcccggc caccgccccc accgcggacg agatcgacgc
     5821 cttcctgcgc gagcacgacc ccaccccccg tcgagtgaac acgtaacgcg cgtcgagtga
     5881 acaccgcaac cccagcgcaa cgccaccacc tgtggacaac gtgttcactc gacgtgttat
     5941 cggtgttcac tcgacggggg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 natcggggcc gcgtgccatc tgacgcgcga actgccgggc acgagttatg caatcctgga
     6121 aatgcgcgag cgcctcggcg ggacctggga cctcttccgc tatccggggg tgcgatcgga
     6181 ctcggacatg tacaccctgg gctacggctt ccggccgtgg cgcggcaggc aggccctcgc
     6241 cgacggcccg tcgatcttgc gctacatcga ggacaccgcc ggcgagtatg acgtccttcc
     6301 gcgcatccgc tacgggcaca aggtggttcg cgccgactgg tccagcgggt cggcccgctg
     6361 gaccgtgacg agcgaaggcc gcgacgcgac cggggagccg cgcacggcga cggcaacgtg
     6421 ccggttcctc tactgctgca ccggctacta cgactacacc gagggctaca cgccgcactt
     6481 ccccggcatc gggagctata ccggacaggt cgtccatccg cagttctggc cggaggggtt
     6541 gccgtatgcc ggcacgcgcg tcgtcgtcat cggcagcggt gcgaccgcga tcaccctggt
     6601 ccccgcgatg gccgccgagg cagcgcacgt gaccatgctg cagcgcacgc cgacctacgt
     6661 cctgaacatg ccgtcggccg acccggtcgc agacctggtg cagggccgac tgccgcagcg
     6721 ggcggcatac ctcatcaacc gctggaacca tctgttgcag tcctggggcc tctaccgtgc
     6781 ggcccagatc gcgcccggcg ccgtccgaaa gctcatccgg acggacgcga aaagtcatct
     6841 gccccaagac ttcccgatcg acgtgcactt caacccgccg tatgacccgt gggaccagcg
     6901 gctctgcctc gtccccgacg ccgacctcta ccgggccatc cgtatggggc gggcggaggt
     6961 cgtcaccgac acgatcgaca ccttcacccc gaccggtatc cgtctcacca gcggccgcga
     7021 actccgcgcc gacatcgtcg tgaccgccac cgggctgaag gtgctccccg gcggcggcgc
     7081 caccctcagc atcgacggcg ccgacgtcga cctgcgggac acggtgtcct acaagggaat
     7141 catgttgagc ggcataccca acttcgcctt ggctctcgga tacaccaatg cgtcctggac
     7201 gctcaaggtc gacctcgtgt cgcgctacat ctgccgactg ctcgcctata tggagcggca
     7261 cggattcgac gaggtgcgcc cgctcgcccc accggccaca gaggcgcggc ggccgatcat
     7321 cgatttcgcc gccgggtatg tccagcgggc cgacccgatc atgccgcggc agggcacgcg
     7381 agggccgtgg cggctgccgc agaactatct ctcggaccgt gcgcggttgc gtcgccgcgg
     7441 tcggctcgat gacggtgtgg tattcacccg ccgcgccgat cgggtcgccg tcaaggcgga
     7501 cgtcgatgac aagcgcgacc taccatgggc gccgtgacga gcgatgagaa ggccaagact
     7561 cccgagaccg acgagttcgg cgggctgatc ggggtgggtt cggcccgcac catccgtgcg
     7621 ccgtggcatc cgtcgtcgat cacacggatc cgggcggccg tgatcgcgga cctccaggcg
     7681 cgcgacgtgg agggcgaaac gctcgacgag gtcgccgtcg tcacgtccga actcgtgacc
     7741 aatgcgctgc gccacgcaac ccccttgcac gacaacaccg tccgcgtcca ctggaccgag
     7801 cgcaacggca tcgtcgaagt cgaagtcacc gacggcggcg gcgagaccga gccccgggcc
     7861 cggcccgagg ccgtcttcgc cgtctccggg cgcggcctgc gcatcgtgcg ctcctacgcc
     7921 cacgagtggg gcgtcagcga gaagaagggg gccgtcaccg tgtgggcggc tttgggtgga
     7981 ccctcgcgtc gtcgggcatg atcgccgtca tgggtaaagc ctcacgcaag aagaaggaca
     8041 ccgccgctcc ccgggcgccc cgccccgcgg catacaacgc ccgtccgttc cgcgacctgc
     8101 ccggcgagag ccagtgggtg gcgatggccg aactccttcc ggcggccacg gtcaccgtgc
     8161 cgctgaaggc cggcgccgtc gaggggggcg cgcgtgaggt cacgatcgtc accgtgctgc
     8221 cgatggcctg gccggccctg caccgcgccg atggcaccct gctcgtggcc acccagggcg
     8281 gcgggcagag cggcgacaaa tcccgcgacc tcgcgagcgc gatactcgcc gccgccgctg
     8341 ctgagccggg cacgccattg ccccaggtgc ccatggccac cgacgcgact ccggcgttgc
     8401 aggatctgct cgacctgacg tcaggccctg aggtgatggt ccacgagggc ttcgacttct
     8461 gggtggaggg tcaggaactc gacgccgccg gacaggagtc gctcgcccgt gccaacgact
     8521 ccgtgctgcc gaccgcgaag gtcgacggcg ccgacgcggc gtattggtgt ctcatcggcg
     8581 accggccgca cgtgcgggcc gtgctcgccg aggacgagga cgaggcgacc aatgccctgg
     8641 cccggctctg ggccgccggc acggcggcac tgggcgacgg cacccggctg ctcggcgcct
     8701 tccgggccgc cggactgctg attcccgtgt gggagttggc ggatggcacg gacgccgacg
     8761 gcgtcgcggc cccgctggcc gagtggaagg cgcggtatgc cgccgccctc accgacgctc
     8821 ccctgacccc cgaggaacgc cgcgccaagg ccggcatcct gaaccgccag gtcaccctgc
     8881 gctgacggca atgcctccga ccaccccggc accggcaccc gggccgacga ccgcgccgac
     8941 ccgcgtagcc gcgatcatcc cggcgaagtc ggaggccgac cggatcgtcg caacggtcac
     9001 ggcgacggcg ggacttccgc atgtggacct cgtcgtcgtc gtcgacgacg gaagcaccga
     9061 cgcgaccgct cgcctcgccg aggaagcggg ggcggtggtc gtgcgacata cggccaatca
     9121 ggggaaggcg gctgcgatga tgaccggcgc caagaccgtt gccgctcgag atgacccagc
     9181 tgcgcccagg gcgctgctgt tcgtcgacgc cgacctgcgg gagagcgcgg ccaatctggc
     9241 cccgctgatg acgcctgtcc tctccggcgc agtggacatg acgatcgcga acatcccccg
     9301 cgccaactcc tccaaaggcg gcgggcgggt ggtgcggctg gcgcagcgga ccatcctcgg
     9361 gcgcaccggc cgggagattg cccagccgct caacgggatg cggtgcctga cgcgcgatgc
     9421 cttccgggcc gctctgccgc tcgcgcccgg ctggggcgtc gaggtgggtc ttaccctcga
     9481 tgtcctccag gcgggactgc gggtggtcga ggtgcccgtc gacttcacac accgggcgac
     9541 cggcaaggac tggcgcggcg cgctgcaccg gggtcgccag ttgcgcgatg tcgcccgggt
     9601 cgtggcccgg cgcggccgtt gaggcgatga gcggcccggc cggccgccgt gcgttgggcg
     9661 ctatcgctgc cagagcgccg ataacgcccc acgcacgccg ggcgggccgt ctcgcgacgg
     9721 ttgccgccct cgcgctcctg ctctcggtcg ggtggctcgg cgagtctgcg gccaagcccc
     9781 cgctgggaga acggggctgg gctcctggat ccttgccgtg gactctcggc ccggccgccg
     9841 tcacgggaat gctcgccgcg gcatacctgc tcggggccgc cggcctctgg ctgggcctcc
     9901 gggaccgggg gggctggccg gggtgggcca tcgcagcggt ggcggccggc gccctcctgg
     9961 cgatgccgtt cggctcggcg gatcacacga actacgcggc atacggccgg atcgcgctgc
    10021 agggcggtga tccctacgtc gaatctccca tcgcgtgggc cggcggcgac gaccccgtga
    10081 cgagcggggt gcagccgccg tggcaggaga cgccgagcat ctacggtcca ctctcgaccg
    10141 ttcttcaggt gttcgccgct tgggccggag gcgacagcct gcgtcaggtc gtctggatgt
    10201 ggcaactgct gatcgtcggc gcctggctgg cgacccgctg gctgttgcgg cggctcttcc
    10261 ccgacgcgtc tggtcgcgtc gacgtgctgt ggacgatgaa tcccttcgtc atcggcgcgg
    10321 gggtgctggg cgcccacgtc gacacgctcg cgacgccgct gatggtgggg gcgctcctcc
    10381 tggcgcggcg gcccggccgg ccatgggcgc tggccgccgg cacgctgggc gggctggcgc
    10441 tttcggtgaa ggtgaccgcg ggcgtgatcc tcgtggcgat cgccctgggg tggctgatcg
    10501 agcgtcgccg ggcggccacc cgcgtcctga ccctcgcggc aggcgcggcg ctcgccgcgg
    10561 tgcctctgca cctgtgggcg ggcgcgcggg catacgacca gctcgggcgt tcgcggcgct
    10621 cgatctccct cgccaccccg tggcgcaaga tctacgaatg gttcgggggg cagttgccgg
    10681 atgcgacgtg gcgctccctg ctgttcgtcc tcgcgggcct gacctgtctc gcgctggcct
    10741 ggctggcttg gcgcgtgctt tgcacgcagg cgtgcgaggt ggacacgccc gtgatgctcg
    10801 cgctggcgct gtcgatcggg tatgcggccg gcgcgcccta tgtgctgccg tggtacgacc
    10861 aactcgtgtg ggcactgctg cccgcggtcg cggcggccgg tctgctggag cgggtatgga
    10921 tcgtgcgcag cctgatcctg gcgctcgcct atgtgcccgg ccgggtggtc gggatgacgc
    10981 cgggcgtcga agaggtgacg ctgatatggc gccgcgacat ggctccgctc gcggctgtgc
    11041 tcgtatggat cgcgctggtg gtggcgtccc ggtcggcctg ccggcccgcg tccgtcactc
    11101 ccaggaggcc tgcgcgaagc ccgcggcagc caccggctcc acgtcggtga accacacctc
    11161 gggcttcggc gcgtcgtatg ccgcgtggac cgggcaatgg ctcagtccac gcgccggaaa
    11221 cccgccgcct cggccgcctc atcgtcggtg aaccagacgt gcggctccgc tgagccgtat
    11281 gctggatgcc cctctgtcac ataggttttg gtgtcctccc acgccatgac ggggtgcccg
    11341 agcggcatgg cgtcatcgtg gatcggcgcg gccgagccga cgctatagcc gccgtcgacc
    11401 acctcctcga actgcgatac ccgccggtcg gcggcaccgg cggtggactg ctgctcggcg
    11461 ttctcgccct cggtgaccaa agtgccctcg gcgtcccata cctgggggcc gccggcatcg
    11521 tcggcggtcc agtcgccggc ctgccggaac tccccgccgg cggcgatatc gtgattggcc
    11581 gggtcgtgct ccgcggcgaa ctcctccggg gagagccggt tgccgccctc gccgggtcgg
    11641 tcgtgcccgg cctcgccggg gtcgccgccg ccatcccgat cttcgcgccg ttcgtcgcgc
    11701 tgctcgtcgg agggggccgg ggtctgtgcc gcgccggggg gcggggttcc cggcgcgccc
    11761 tcggcgtcgt cgaacacctc agtgttgctg gacccgagtg ccggggtggc ggagttcgcg
    11821 gtctcgtcgt ccgcctgccg atcgaggtca tcgagcgcgc gctcggtgcg ctcgtgcgcg
    11881 tcgtttgcga gggtggcatc tccggctcgg ggatcgctgt ccggcgtccc ggctgggccg
    11941 tcgacaccct cgcgcgcgac gttgtcgccg cccgccgggg gttccgcgga gtatgcggtg
    12001 gcgcctgata gctcgccggt gcccaacggc tcgttgccgc tcgtgtcatc gatggaggtc
    12061 atgaccgtca cccacccttg tcagtgatcg tcggatatct cgatgtcgta cttcttggcg
    12121 gcccgcttga tgcggccctt gatggtctcg acctcgtcgg cgtcgtactt gtccgcgttg
    12181 gcctgcttgt tgatgtagga ccacgccgca cggacatgct cctcggagtc gatggggtac
    12241 ttcttgttcg ttgggtcggc gaacgtgaca tcgccgtgtt cgcgttcccc atcgcggggg
    12301 ttgacgtcgt cccgacggga gatgtggttg ctcacacacc gacggtaggg ccgtgagcac
    12361 cgtcacaggc ctcttgtgac ccggcgaatt gaccgcctcg agcacgtggg ttgccacagc
    12421 tcggccctgc cggcagcccc ctcggcttag gttgtgtcca tgccgatgca acctctcgag
    12481 ctgcccgggc cgcatggcga ctgggctcag ttcgtcgacg acctggtcga gtgcggtctc
    12541 gccgatgcgc gccggatcgc caccggcctg aaggacgggg caccgcgatc cgcccatgag
    12601 gtgctggatc gctggaacga ctcgtccatc gccgtgtcga cggcgggcgg ggtcgcgggg
    12661 ttgctcgcgc aggtgcatcc ggaggtggaa gtccgcacga ggtccgaggc cgcgctgcaa
    12721 gagctggcca agttcgggtc agagctgtct ttggacaagg agctgtatgc ggtcttcgcc
    12781 gcggtcgacg gcggtggact ggatcccgaa gcggctcggc tgctcgcgaa ggtgttgcgt
    12841 gacttccggc gctccggcgt cgaccgcgac gacgcgaccc gtgaccggct caaggcgatc
    12901 gacgagcgcc tgaccgttct cggacaagac ttctcgcgca tcatccgcga cgatgtgcgg
    12961 accatccgga tcgctcctga gcgtctggac ggcctgccca aggactacgt cgacgctcac
    13021 ccgcccggcg acgacggatt ggtggcgatc acgaccgatt atcccgacct catccccttc
    13081 accacgttcg cacacgacgg ggcggcgcgg cgggaactcc atacggcctt tctcaaccgg
    13141 ggctggccgg acaacgaccc ggtgctgcac gagatcctgg acctgcgcca cgaaatggcg
    13201 acgctcctcg ggtatcccga ctgggccgca tacgacgccg aggtcaagat gatcggcacc
    13261 ggggaggcga tcggcgaatt catcgatcgc atcacggacc ttgccgacgc gccgggtcgg
    13321 cgcgatctcg cagtgctgct ggcgcgcgcc cggcaggacg atcccggcat cgaggcgctg
    13381 accgcggccg acaacgccta ctacagcgaa ctggtgcgtc gcgaagacta cgaggtggat
    13441 gcccaggagg tgcgtcgcta cttcgacttc gccaaggtca gcgccggctt gttggacgtg
    13501 acgggccggc tcttcggcgt gagttaccgg ccgcgcgacg acgcgccggt ctggcacgag
    13561 gcggtcacgg cgtacgacgt gctgcgcggc gacgagatga tcgggcggat ctatctcgac
    13621 ctgcacccgc gggaggggaa gttcaagcac gcggcgcagt tcgggctggt cagcggggtg
    13681 gccggccggg atctgccgga gggggcgctg gtctgcaact tcccgactgg gctcatggag
    13741 cacacggatg tggtgacgct gttccacgag ttcgggcacc tggtccatca cgtgctgggt
    13801 ggcgcacagc ggtgggtgcg cttcagtggc gttgcgacgg aatgggattt cgtcgaggcg
    13861 ccctcgcaga tgctcgagga atgggcctgg gatgccaacg tgctgcgatc gttcgcgacc
    13921 gacgcggacg gggtggcgat ccccggggag ctggtggcca agatggtcgc ggcgcacgag
    13981 ttcggcaagg gccggcacgc gcggacgcag atgttctatg cggcgacttc ccttgggctg
    14041 caccgcgatc gacccgccga ccatacggcg tatgtcgcgc agctgcagga gcgatatgac
    14101 ctgctcgccc gactgcccga tacccacttc caggccgcgt tcggccacct cgacggctac
    14161 gggtcgggct actacaccta catgtggagc ctggtcatcg ccaaagacat gttcagcgcc
    14221 ttcgaccgga caaacctctt cgaccccgag gttgccgaac gctaccgcga tcgcgttctg
    14281 gcacaaggcg gccgcgcgga tgcggccgac ctggtcgccg acttcctcgg ccggccgtat
    14341 tccttcgaca ccttcgggga atggctcgcc cgctgaagcc acaggtcgcc taggccaggg
    14401 cagcgcccag gacgcaggcg gccagcccga gggcgatgct gagcacgacg ttgccgacgg
    14461 cccggccgcc cgcccgctca tcgaggaggc cgacggtctc gctgctgaac gtgctgtagg
    14521 tcgtcagggc gccacagaat ccggtgccga gcagggctgc gagatgaccg tcggtgcgac
    14581 cgaggatcag gccgaggagg aagctcccgg cgacattgac gacaagggtg ccccatggga
    14641 agccgcggcc ggccacgcgg gcgacgaccc ggtcggtcag gtagcggccc aatgcgccga
    14701 ctgcgccccc gatggcgacg aggaggacgg tcacgccgtg ccctcctcgg caggtgtggg
    14761 caggtcgccg gcctcgtcct cacgcgggag cggccagccg aggatgcggt gcatcgcctg
    14821 cacaccgagc cagcaggcgc cgatcgcagt gacgggtgtc aggacgagat agcttgcggc
    14881 agcgaggctt tcgcccgacg tccagagcgc acgcgtgtcg taggcgaagg tggagaaggt
    14941 ggtgaaacca ccgaggaaac cggtgccgag gaaggggcgc agcagccgat gcgccggccg
    15001 gcgcacgacg aagcacacga gcagtcccat gagaaacgac cccacggcgt tgacgatgag
    15061 gaccgcatcg gagactccgc cccagccgac gtcgaccgcg cgcgtcaacc cgtcccagga
    15121 ggccacggca taccgcgcga cggcccccag cgcgccgccg gccgcgatcg ccgccaggat
    15181 gtcgccggcg agcgatgggt agcgacggcg ggcggacggc atacgaccat cctgccaagc
    15241 ggtggtgtca gccgccgaag ccggccatcg cggccactcg ctcggccaac tcctcgtcga
    15301 cccggatggg gctgccgtcg acctcgacga tcggcgaagg gccacggcct gaggagacga
    15361 gccagacgcc gtcggcggtc agcaggtcgc cggcggggac gagttcgacg cggacaggca
    15421 tcccggattc ggctgcggca gcgaggatct cgtcgacggt gacgctggcg aggatgccgg
    15481 tgtcaccggt cggcggagtg cgcatgacgc catcggtgac aacgatgaga cccgatgtcg
    15541 ggccgtccat ggcgaacccg tcggtgctga cgaagatggc gtcatcggcg ccgcgccgct
    15601 tggcctctcg cgccgcggcg acattgccga cataggacaa tgtcttgacg ccacccagca
    15661 gccagggcgc gtccttgaag gcatccgagg gccggccact ggtcagcgtc accgcgcgca
    15721 gggcgcgggg gacccggcct cggggcctgg ccggcttctc ggtgatcgac agcagggcca
    15781 acggcccggc gtcgggagcc cactcgcggc cgcgggtcag cacgagcttg aggacggcct
    15841 cgccggaccc gtgccaagcg accagcgcct ccgcgacgag ggcctcccat gcgtcccgtg
    15901 tcggaccggc gatgtcgagc gcggcggcgg cggcgtcgag ccggtcgagg tgctcgccca
    15961 agttgacgac ccgcgctgcc ccgtcggtgt cggtgagcac acgcgccgag tcgaaacagc
    16021 cgtccccgcg ggcgaatccg aggtcgtcga cggtgaccac cggttcgtcc gcacccacga
    16081 caccgcggcc cagaaccgcc acgagatgag cgctcatcgc gtaattgtct cgcgggcctt
    16141 cgtggcgtga ctaggtcagc aggccgttct cccgggccag attggcgatg ccgacccgcc
    16201 cggtcgcgtc cagcttcgtc gtgatcgccg acacctgatt gcgcacggtc ttgtccgaga
    16261 tgccgagacg atctgcgatc tgcgaattgg tcgcgccggt ggcgacgagc agggcgatcc
    16321 gcacctcccg cggcgagagt tgccgaaacg gtccgccggt catgccccga tccgtccgag
    16381 cgacgccgag ttgctccccc aacaccaccc cgccgtccgc gacggtgcgc agcgcggcga
    16441 ccaggtcttc gggcggggtg tccttgagca ggtagccggt cgccccggcg tcgagggccg
    16501 cgcgcaccag acgcgcgtcg ccatccatcg tcaagacgac cgaacgacag ccgggggcgt
    16561 atgaagtgag ggccttgacg acgctggtcc cgctgccgtc ggggagtttc agatcgacga
    16621 cggcaaaggt cggcgacgcg ctgtggatga acgtcagcgc gtcgccgacc gtggccgcct
    16681 cgcggatctc tccggcccag ctctcccccg tcagcagggc caccagcccg cggcggacca
    16741 cgggatggtc gtcgacgacg agcgtcaaac ggtgcggatc aggcgaggcg gtcacgagta
    16801 cagcctcacc cgatcctggg cccatcgacc gggacaacgt cccggctcgt gcggggctgc
    16861 gcggcgcgag ggctggctga gagcggcagc accacccgca ctgtggtgcc ctcgggtgtg
    16921 ccggagatct cgcacgaccc accaagttcc tcggcccggg cccgcatgct cgccaatccc
    16981 accccggccc ccgggccggc ctgaacaccc gggccgtcgt ccccgaccag gagcaccagg
    17041 tgttgtgccg cccggacgga cacggtgcac cgggtgacgc cgaccgcgtg ccgagccacg
    17101 ttcgtcaccg cctcggtgac gatgcggtag gcggcaatct cgacggccgg cggcatgggc
    17161 gccagctcgg ccggcgcgtc gagaacgacc
//
LOCUS       DAOPJG010000054        17135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3527_length_17135_cov_6.712998, whole genome shotgun sequence.
ACCESSION   DAOPJG010000054 DAOPJG010000000
VERSION     DAOPJG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17135
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3527_length_17135_cov_6.712998"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKU75_06990"
     CDS             complement(<1..333)
                     /locus_tag="PKU75_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80658.1"
                     /translation="MGFLDNLKDGLGFDSDEEKAQKAAEKAAEEAKDRAEEAAKQAKE
                     AADAQAKANEAKAKADEATRKSAPAPAPTSTPAPAPKPTPEPKVAPAPDYTKSVPAPE
                     PEAAPAPPK"
     gene            530..603
                     /locus_tag="PKU75_06995"
     tRNA            530..603
                     /locus_tag="PKU75_06995"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Ile,seq:cat)
     gene            693..1079
                     /locus_tag="PKU75_07000"
     CDS             693..1079
                     /locus_tag="PKU75_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80659.1"
                     /translation="MTHISTTLIGSMPRGEELTPLLIARDNGQPYDAAEFDRLVSAAT
                     DDAVAKQVECGVDIVSDGEMGKVGYSTYMIERLSGFGGHVDRKPAADLAAHPGLGKKL
                     SAIMGNQEFVRASCVGKVELVDDKPM"
     assembly_gap    1286..1385
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1516..1953
                     /locus_tag="PKU75_07005"
     CDS             1516..1953
                     /locus_tag="PKU75_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPF80660.1"
                     /translation="MRASGPSGVDAAERRAIEHDCAALVMRYANGNDAADWDLVARLF
                     AADGRMSRPTAPDDFIEGRDAILAAFRARPARTTRHVTSNVVIDVESATSARGECALV
                     LYTAYKAPIVASFHDRFVRTSDGWRFAERRGSLTFAAETGAPA"
     gene            2087..2602
                     /locus_tag="PKU75_07010"
     CDS             2087..2602
                     /locus_tag="PKU75_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HPF80661.1"
                     /translation="MSYAPIRGATRGVVFIHSTPAALCPHLTWALEAVLGGRVLLDWT
                     EQGAQYGLKRTEISWTGSAGTGAKLTSALKAFTGIRFEVTEEPSPGTNGSRWSYTPSL
                     GLHHGWMAPNGDTMVNEERLKEVILYAQGSNEAVTDMLEELLGTAWDEELEIFRYAGE
                     GAPVRWLHKVG"
     gene            complement(2688..3944)
                     /gene="fabF"
                     /locus_tag="PKU75_07015"
     CDS             complement(2688..3944)
                     /gene="fabF"
                     /locus_tag="PKU75_07015"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPF80662.1"
                     /translation="MSAGSSDTKVVITGLGATTPLGGDMPTTWEALLAGTSGARTIEA
                     DWVAKYELPVTFGAPIHTPADQALTKVEVRRLDPNAQYAMIASREAWQDAGAPEVDPE
                     RLGVAIGTGIGGVWTLLDQWDNLREKGPRRVFPLAVPMLMPNSATGNVSLDLGARAGA
                     HTIVSACASGAEAMGFAAEMIRSGRADVVVAGGTEGAMHPLCFAGFAAMQALSKRNDD
                     PAHASRPYDVARDGFVMGEGAGIMVLESEAHAKARGARIYAEFAGLGMSADSHHIAAP
                     EPEGAGASRAMTEAVRNAGLGASDIIHINAHATSTPVGDVAEAAAIRRAFGEAADGMP
                     VSATKSMTGHLLGAAGALEGLFTVLAVHHRVAPPTINIENLDPEVDLDVIRDTPRELP
                     AGDIAALNNSFGFGGHNVALAIKSYS"
     gene            complement(4031..4279)
                     /locus_tag="PKU75_07020"
     CDS             complement(4031..4279)
                     /locus_tag="PKU75_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPF80663.1"
                     /translation="MAQTEQEILEGLANIVNEETGVDVADVQLDKSFTDDLDIDSLSM
                     MTIVVNAEEAFGVRIPDDEVKNLSTVGDAVTFIKNAQA"
     gene            complement(4361..5392)
                     /locus_tag="PKU75_07025"
     CDS             complement(4361..5392)
                     /locus_tag="PKU75_07025"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPF80664.1"
                     /translation="MRLRKSRSPKGTGKIKGATGSSGSRITGVGGYRPERVIPNSEIV
                     DAIDSSDEWIRERSGITERRFARPDESVVDMAEAASKGAIAMAGIDQSDIDAVLVATV
                     THPYQTPAAAPILADRFDLRCAAFDISAACAGYCHGISLASDMVRGGSASNVLVVGVE
                     KLSDFTDMTDRSTAFIFGDGAGAAIVSRSDKTKIGPTVWGSEGDSWQTIMQRESWTEF
                     RGNDEKTWPTIGMEGHKVFRWAVWGMAPVAQKAMEAAGVKASDLDAFIPHQANMRIID
                     AMIKQLKLPEDIVVARDIATTANTSAASIPLATERMLREGQVQSGSLALQIGFGAGLV
                     YAAQVVELP"
     gene            complement(5389..6342)
                     /locus_tag="PKU75_07030"
     CDS             complement(5389..6342)
                     /locus_tag="PKU75_07030"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPF80665.1"
                     /translation="MLAIVCPGQGSQTPGFLTPWLELPGLRENAEEFSDATGIDVVEH
                     GTESDEETIKDTAVAQPLIVAAGLLTYAALAEAASVADAGSADLLAGHSVGEITAAAI
                     AGALSDEEAARFVAIRAKGMASASAITPTGMSAVLGGDPDVVLAAVEAHGLTPANNNG
                     AGQIVAAGTLEQLAALAEALPAKARIIPLKVAGAFHTQHMAPAVDALAAYADLLTPSA
                     PRIPILSNADGGSLTDGQEILSRLVSQVTNPVRWDLCMQSMLDAGVTGLIELAPAGTL
                     VGLAKRGMKGVETLAVKSPDDLDAAAAMIRNHSTTTEGTAE"
     gene            complement(6433..7647)
                     /locus_tag="PKU75_07035"
     CDS             complement(6433..7647)
                     /locus_tag="PKU75_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF80666.1"
                     /translation="MPRRAALPAKTRREIAANTASLVRAAVADIEARHGWYAALSARD
                     RASVALVAHSGIENFVAWLSDESTVQVPAQALFDAAPPELTRSISLAQTLDLIRSVVD
                     VVEVQAAELVGPETAPALLEAMLRYSRDVAFGAAHIYARAAEQRGAWDARLEGLVVDA
                     ILRGDADDSIQSRAAALGWGATTRVAVVAGAQPEADHAVAIDPPLVLDRLHRAAARNR
                     LQVLAALQGGRLIVLIGETADALRDAALLTDCFGAGPLVVGPTVPHLFAAGRSARAAI
                     SGQAAAAAWPDAPRPAPASELLAERALIGDMPARSALVTRIVRPLAEAGGGELLRTAT
                     AYLDGGRNLEATARVLFVHPNTVRYRLGRIAELTGYDLGDPHDADTVHLAIRLGRLAE
                     AGISGTASVARS"
     gene            7926..10541
                     /locus_tag="PKU75_07040"
     CDS             7926..10541
                     /locus_tag="PKU75_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HPF80667.1"
                     /translation="MSRRRLRLPVVTGLVGAGTTLGLLATSAQALPSAVGDEPYRPAI
                     HFTPARNWMNDPNGLVYYKGTYHLFFQHNPRGDRWGNMSWGHATSTDLVHWREQPLAI
                     PQTLNAAGESVEDIFSGSVVVDKTNSSGFGTATNPPLVAIYTSAYTDKHPTLAGKQAQ
                     SLAYSTDGGMTWTKYAGNPVLNRDSANFRDPKVFRYSGPKGTYWVMAAVEAQEHRVLL
                     YKSRDLKNWTYLSDFGPANAVGGQWECPDLFQLQIQGTTTRKWVMVVNINPGSVAGGS
                     GGQYFVGNFDGERFVSESTVSADTLPPGTSFASFDDGTYGDWTVSNEPGNWANGPWGD
                     APATGTLPGQNPVSGFVGSGLVNGFLDHDWPVGTMRSPEFTVDAAYLNFLVGGGNHPH
                     VAGTQLSNDPPAGSLLWDGFEFPDGTTLADTGWQATGDFATEPWRNPSTAGGDYYIGR
                     KRINTWEGGPRGDDNLGSLTSPAFEITADHLSMLVGGGRRTDGSLEVQLLVGGQVVRS
                     LTGPEGGALNWRSWDVADLRGQQAQLRVWDQAAGGWGHLTLDHVVMGDTPAIPRSDET
                     AVNLVVDGKVVASATGANSEYLDWHSFDLRPYAGRTAYLEIVDNNRFGWGHILADEFR
                     FGAQPAATRAESYDWLDWGRDYYAAVSWNDVPGGKRVMIGWMNNWDYANDIPTSPWRS
                     AMSLPRHVWLAQTPKGPRIVSRVVPQAAALDLTGQAYAAPAQSVSGSRLLPKRGSVAR
                     IDAVLRPGTAKRFGLTVHAAADGSEKTVIGYDTTTGRLFVDRTDSGNVGFHPAFPSVE
                     DAQVSLVGGKLTLSVYLDRSSVELFAQGGRTAITDQVFPSAGSNRIGMWAEGGAMRVE
                     SLKITPLKASMFTGR"
     gene            10553..11266
                     /locus_tag="PKU75_07045"
     CDS             10553..11266
                     /locus_tag="PKU75_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HPF80668.1"
                     /translation="MIEPITPTTPRPVRRAVLTQWWRDLAILHWPVAPHLVAPLLPPG
                     TTPDTRGGVTYAGLIAFEMDRVGPGFGVPFFGRFPEINVRLYSVDAQGRRGVVFCSLE
                     AARLAPVMVGRALGLNYRWARMRMTRTSGGVWRHSSRRIGGSPGRCRLAVEPHHEIAD
                     DDLTHWLTDRWGLHVRRVGRSLYVPNEHPPWRMQTASLLELDETLITAAGLSVDPGAP
                     VSVLYASGVPVRFGMPRPV"
     gene            complement(11284..12699)
                     /gene="argG"
                     /locus_tag="PKU75_07050"
     CDS             complement(11284..12699)
                     /gene="argG"
                     /locus_tag="PKU75_07050"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPF80669.1"
                     /translation="MSKVLTSLPVGERVGIAFSGGLDTSVAVAWMREKGAIPCTYTAD
                     LGQYDEPEIDQIPGRAGQYGAELSRLVDCTSALVEEGLSAIACGAFHIRSGGKAYFNT
                     TPIGRAVTGTLLVRAMHEDQVSIWGDGSTFKGNDIERFYRYGLLANPALRIYKPWLDA
                     DFVGELGGRHEMSAWLSQRDLPYRDSQEKAYSTDANIWGATHEAKTLEHLDVSLETVE
                     PIMGVRFWDPAVSIATEDVSITFVQGRPVAINGADLDPVALVRQANAVGGRHGLGMSD
                     QIENRIIEAKSRGVYEAPGMALLHIAYERLLNAVHNEDTVANYHAEGRRLGRLLYEGR
                     WLDPQSLMLRESLQRWVASAISGQVTLRLRRGDDYTIVDTRGDNFSYHPDKLSMERVE
                     DAAFGPVDRIGQLTMRNLDIADSRSKLELYAAQGQLTGGQQVLVGQLEAGGAAQIASN
                     PATDPSTDDALDAAAMEFGTD"
     gene            12850..13587
                     /locus_tag="PKU75_07055"
     CDS             12850..13587
                     /locus_tag="PKU75_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80670.1"
                     /translation="MQRRKFIASAALAAAMTGSLVTADAATAAPESGQRAGWTSTFNG
                     SKAGWQNVVGTWVLKPGNLYNTGIAGKRTSVKHINNYTNFYYTVRMKRWGNSTGVAAN
                     AIVIRGNPSSRDGSGYWRPSYLLQYSNNGYYSVWRINADATQTAIQGWTPVSGVISKS
                     GYNKLEVWAYGDSLDFHINGVHLWSGFDSALTFGNVGVSQYTEPGKWSQTWLDSASLT
                     VATRVAPHDHGATGTPVKGGTMDMAPR"
     gene            13693..14421
                     /locus_tag="PKU75_07060"
     CDS             13693..14421
                     /locus_tag="PKU75_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPF80671.1"
                     /translation="MEILDALAAEEDQLAALLAGLTQEQWGADSLCAGWSVADVVLHL
                     AQCEEAVIATIRGTPVDWGGLGDTVGAAMESAVSAERSDSGATICERWERARGQALTV
                     LRAADPARRVAWVTNTLRPATLATTRLAEHWAHALDIAEPLGLPYPDTQRLRHILWLA
                     HATLPYAYSAAGSTAPSVRIEAVAPNGPAWVLGPDDADVMLSGPAGDLARIAARRLDP
                     GDSAVRTSGAAAAEVLVRIRTYAI"
     gene            14528..15517
                     /locus_tag="PKU75_07065"
     CDS             14528..15517
                     /locus_tag="PKU75_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPF80672.1"
                     /translation="MEPLVPVIVAHRGACGYLPEHTLAAYELGIRLGADAFEIDVVPT
                     TDGHLVARHENELSVSTDVATRRGFAERRTTKLIEGAAQDGWFAEDFTLVELRQLLAR
                     QPLRGLRSTAHDDAYAVASLPEILALRSRLAAELARPVGLKLEVKSPAHFAAIGHPMA
                     DRLITDLDAAGMLGADSPVEIMTFEPAFLRCLRARGVDNDLVLLVEEDPTRPVPGEGE
                     TTFADLTTPASLAELASLITGIGPGRHALFSGLPGGRLGEPLPLFADAKAAGLRLDCW
                     TFGAENAFLPTDFRIGDDPAAYGDMAGFVAAYREQGLDTVITDHPDLVIPTPR"
     gene            complement(15586..>17135)
                     /locus_tag="PKU75_07070"
     CDS             complement(15586..>17135)
                     /locus_tag="PKU75_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HPF80673.1"
                     /translation="IKGYTLGSHFAGRNATHQMKKLTLDDLKGFRDTLHIPITDAQLE
                     ADPYQPPYYHPGTEDEAIQYALEMRGKLGGGLPRRKVDYAPLQLPGDDAYKAVKKGSG
                     KQQVASTMAWVRLLKDLMREKDFGQRVVPIIPDEARTFGMDSFFPTIKIYNPHGQRYR
                     SVDADLMLAYKESEAGQIVHTGINEAGSMAAFTAAGTSYSTHGEAMVPMYIFYSMFGF
                     QRTADSIWAAADQMSRGFLLGATAGRTTLTGEGLQHADGHSHLLASTNPAVRAYDPAF
                     GYEIAHIMKRGLEQMYGETGDGDEKNVIYYLTLYNEPMVQPAEPENVDVEGIVKGMYR
                     LADADRSGRENNPSVQLLASGVGVPWVMEAQQLLRDDWGVNADVWSVTSWNELRREAL
                     EVDRTRFLHPEQQVADPYVTQRLSGMPGPVIAVSDYMRAVQDQIAQWVPSDFHSLGAD
                     GFGFSDTRPAARRYFHIDGPSVVVRALQALAARGEIDASVPGQAAEKYRLLDVTAGAS
                     GNAGGDA"
BASE COUNT         2581 a         5714 c         6051 g         2689 t
ORIGIN      
        1 cttcggcggc gccggggcgg cctcgggctc gggagccggg acagacttgg tgtagtcggg
       61 cgccggggcg accttgggct cgggcgtcgg cttgggggcc ggcgccgggg tggaggttgg
      121 ggctggggcg ggagcggact tgcgggtggc ctcgtcggcc ttcgccttgg cctcgttcgc
      181 cttcgcctgg gcgtcggcgg cctccttggc ctgctttgct gcttcctcgg cgcggtcctt
      241 ggcctcctcg gccgccttct ccgctgcctt ctgcgccttc tcctcgtcgg agtcgaagcc
      301 gaggccatcc ttcaggttgt ccagaaaacc catgatcggg tgatcttcct tccgtatgtg
      361 agcgccctgg cgggcacccg ctgacggggc cgggagtgac cccgcggttg gagcaattta
      421 ccgtaggccg gcatttccgg cgagggctag cggtcggtcg atttctgtcg ggcgccgggt
      481 ggctcgatag gatctgcccg ccggcccgcg cccgtgtcgc ggccggtgcg gggcgttagc
      541 tcagtcggtc agagcagcgg actcataatc cgtcggtcca gggttcaagc ccctgacgcc
      601 ccaccctgcg ccggggtcgc ccggtcctct cggctgccgc actgggctgg ccggcacccg
      661 gcattgacac cggctccggg cgagaggacg tcatgactca catcagcacc actctgatcg
      721 gttcgatgcc ccgcggcgag gagctcactc cgctgctcat cgcccgagac aacggccagc
      781 cgtatgacgc ggccgagttc gatcggctgg tctcggccgc gaccgacgac gccgtcgcca
      841 agcaagtcga gtgcggcgtc gacatcgtca gtgacggcga gatggggaag gtcggctatt
      901 ccacctacat gatcgaacgg ctatcgggct tcggcggtca cgtggaccgc aagcccgcgg
      961 ccgacctggc cgcccatccc gggctcggca agaagctgtc cgcgatcatg ggcaaccagg
     1021 agttcgtccg cgcctcctgc gtgggcaagg tcgaactcgt cgacgacaag ccgatgtagg
     1081 cagacgtcac ccggttcaag gcggcactgg ccaaggccgg ccggacaccg gccgacggat
     1141 tcatgaacgc agcctcacca ggcgtgatct cggcgtttca ggtcaaccag tactacccct
     1201 cacacgaggc gtatgtcgtc gacctcgccg aggcgatgcg gcacgagtac gagacgatcg
     1261 tcgccgaagg cctcaccctc caactnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnncagag cgcgtgatcg cctcgacgga ctgcggtttc gggaccttcg cgggctacgg
     1441 caagctcgac cccggggtga cgctgaagaa gctgcaggca ttgcgagcgg gcgcggacct
     1501 cgcgggagca cggctgtgag agcctcgggc ccctccgggg tggacgccgc cgagcggcgc
     1561 gccatcgaac acgactgtgc cgccctggtc atgcgctacg ccaacggcaa tgatgcggcc
     1621 gactgggatc tcgtggcccg gctcttcgcg gcggacggcc ggatgtcccg gccgaccgcg
     1681 ccggacgact tcatcgaggg acgggacgca atcctggcgg cgttccgggc gcggccggcg
     1741 cgcaccacac ggcacgtcac ctcgaatgtc gtcatcgacg tcgagtcggc gacctccgcg
     1801 cgcggggagt gtgcgctcgt gctctatacg gcgtacaagg cgccgatcgt cgcctccttc
     1861 cacgaccgct tcgtgcggac ctcggacggc tggcgattcg cggagcggcg cggctcgctg
     1921 acctttgcag ccgagaccgg cgcgcctgcg tgaaagctgg gcccggcctg catatggtgc
     1981 ctgcacgcgt catagggcac aatgtgacgt gacgagatca ctgtgactcg tggtgtgcaa
     2041 gttggcgttg gggaagacgt ccacccgcac accaggaggt ccagggatgt cgtatgcacc
     2101 gattcgtggt gccacgcgcg gggtcgtgtt cattcactcg acccctgccg cgctgtgccc
     2161 gcacctgacc tgggctctcg aagcagtgct cgggggccgc gtgctgctcg attggaccga
     2221 gcagggcgcc cagtacggcc tgaagcgcac ggagatctcg tggaccggtt cggccggcac
     2281 cggggcgaag ctgacgagcg ctttgaaagc cttcaccggc atccgcttcg aggtgaccga
     2341 ggagcccagc cccggcacca acggttcgcg ctggagctac acccccagcc tgggcctgca
     2401 ccacggctgg atggcgccca atggcgacac catggtcaac gaggaacgcc tcaaagaggt
     2461 catcctctat gcccagggca gcaacgaggc cgtgaccgac atgctcgagg aactgctcgg
     2521 cactgcctgg gacgaagaac tcgagatctt ccgatatgcc ggcgaaggcg cccccgtccg
     2581 gtggctgcac aaggtcggct agccagtccc tgcttcgtca tgtgagaggt agcgacgtca
     2641 tagcgtcgct acctctcaca tgaggtgtca ccatcaccgc gggatggtca ggagtagctc
     2701 ttgatcgcca gggcgacgtt gtggccaccg aagccgaagg agttgttgag cgcggcgatg
     2761 tcgccggccg gcaactcgcg gggggtgtcg cggatgacgt cgaggtcgac ctcggggtcg
     2821 aggttctcga tgttgatggt cggcggcgcg acgcggtggt gcacggcgag gacggtgaac
     2881 aggccttcga gggcgcccgc cgccccgagg aggtgaccgg tcatcgactt ggtggcgctg
     2941 accggcatac cgtctgcggc ctcgccgaac gcgcggcgga ttgcagccgc ctcggccacg
     3001 tcaccaaccg gcgtggaggt ggcgtgggcg ttgatgtgga tgatgtcgga cgcgccgagg
     3061 ccggcattgc gcaccgcctc ggtcatcgcg cgtgaggcgc cggcgccctc gggctccgga
     3121 gcggcgatgt ggtgggagtc ggcgctcatc ccgagaccgg cgaactcggc gtagatccgg
     3181 gcaccgcggg ccttcgcgtg cgcctcggac tccaggacca tgatccccgc gccctcgccc
     3241 atgacgaagc cgtcgcgggc cacgtcatac ggccgcgacg cgtgagccgg gtcgtcgttg
     3301 cgcttcgaaa gcgcctgcat cgcagcgaat ccggcgaagc acaacgggtg catcgcgccc
     3361 tcggtgccgc cggcgacgac gacatccgcg cggccggagc ggatcatctc ggcggcgaaa
     3421 cccatagcct cggcgcccga cgcgcaggcg gagacgatcg tatgggcgcc cgctcgcgca
     3481 ccgaggtcga gcgagacatt gccggtggcc gagttgggca tgagcatggg cacggcgagc
     3541 gggaaaacgc ggcggggccc tttctcgcgc aggttgtccc actggtcgag gagggtccag
     3601 acgccgccga tgccggtgcc gatggcgacg ccgagacgct cggggtcgac ctccggggcg
     3661 ccggcgtcct gccaggcctc gcgggaggcg atcatcgcgt attgggcatt ggggtcgagc
     3721 cggcggacct cgaccttggt gagcgcctgg tcggccgggg tgtggatggg cgcgccgaag
     3781 gtcaccggca actcatactt cgccacccag tccgcctcga tggtgcgcgc cccggaggtc
     3841 cccgcgagca gcgcctccca cgtcgtgggc atgtcgccgc cgagaggagt ggtagctccc
     3901 aagccggtga tgacgacctt ggtgtcgctg gatccagcgc tcatgtgcgg actctccttg
     3961 tgggcgggtg ctgggagggg gccggatgcc gtgcgccctc gcggtcgcgg gcgcacggca
     4021 tacggggctg tcaggcctgg gcgttcttga tgaaggtgac ggcgtcgccg acggtggaga
     4081 ggttcttgac ctcgtcgtcg gggatgcgca cgccgaaggc ctcctcggcg ttgacgacga
     4141 tcgtcatcat cgacagcgag tcgatgtcga ggtcgtcggt gaaggacttg tcgagctgaa
     4201 cgtcggcgac gtccacaccg gtttcttcgt tgacgatgtt cgcgagaccc tcgaggatct
     4261 cctgctcggt ctgagccatt gttctctcct tgtgtcggat tgttgtgtcg gatgtgattc
     4321 ggcccgatcg acccgtggac caccgggaac ttgttgcgcc ctaaggcaac tcgacgacct
     4381 gcgcggcata gaccaggccg gcgccgaaac cgatctgcag cgcgaggctg ccgctttgga
     4441 cctgcccctc gcgcaacatg cgctcggtgg cgagggggat ggaagccgcc gaggtgttcg
     4501 ccgtcgtcgc gatgtcgcgg gcgacgacga tgtcctcggg cagcttcagc tgcttgatca
     4561 tcgcgtcgat gatgcgcatg ttggcctggt gggggatgaa cgcgtcgaga tcgctggcct
     4621 tgacgccggc tgcttccatc gctttctgcg cgacgggcgc catgccccag acagcccagc
     4681 ggaagacctt gtggccctcc attccgattg tcggccaggt cttttcgtcg ttgccgcgga
     4741 actcggtcca ggactcgcgc tgcatgatgg tctgccagct gtcgccctcc gacccccaca
     4801 cggtcggccc gatcttggtc ttgtcgctgc ggctgacgat ggcggcgccg gcgccgtcgc
     4861 cgaagatgaa ggcggtggag cggtccgtca tgtccgtgaa gtcggagagc ttctcgacac
     4921 cgacgacgag gacattgctg gcgctgccgc cgcggaccat gtcggaagcc agcgagatgc
     4981 cgtgacagta gcccgcgcac gccgccgaga tgtcgaacgc cgcgcaccgc agatcgaagc
     5041 gatcggccaa aatcggtgcg gcagcggggg tttggtaggg atgggtcacg gtcgcgacga
     5101 ggacggcgtc gatgtcggac tggtcgatgc cggccatcgc gatggcgccc ttgctcgcgg
     5161 cctcggccat gtcgacgacg ctctcgtcgg gacgggcgaa ccggcgctcg gtgatgccgg
     5221 agcgctcgcg gatccattcg tcggaggagt cgatcgcgtc gacgatctcg gagttgggta
     5281 tgacgcgctc gggccgatag ccgccgacgc ccgtgatgcg tgatccggat gacccggtgg
     5341 cgcccttgat cttgccggtt cccttgggag agcgggactt tcgcagcctc attcggcggt
     5401 gccttcggtc gtggtggagt ggttgcggat catggcggcg gcggcgtcga ggtcgtcggg
     5461 ggacttgacg gcgagcgtct cgacgccctt catgccgcgc ttggccaacc ccacgagcgt
     5521 gcccgcgggg gccagttcga tcaggccggt gacgcccgcg tcgagcatgg actgcataca
     5581 caggtcccag cgcaccgggt tggtgacctg ggagaccagg cgggagagga tctcctgccc
     5641 gtcggtgagg gagccgccgt cggcgttgga caggatcggg atccgcggag cgctcggggt
     5701 gagcaggtcg gcgtatgccg cgagggcgtc caccgcgggg gccatgtgct gggtgtggaa
     5761 ggcgcccgcc accttgagcg ggatgatgcg ggccttggcc ggcaaagcct ccgcgagtgc
     5821 tgccaactgc tccagggtgc cggcggcgac gatctgcccc gcgccgttgt tgttggcggg
     5881 ggtcagcccg tgcgcctcga cagcggcgag gaccacgtcg gggtcgccgc cgaggacggc
     5941 cgacatgcct gtcggagtga tcgcgctggc tgacgccatg cctttggcgc gaatggcgac
     6001 gaagcgcgcc gcctcctcgt cgctcagggc cccggcaatc gccgcggcgg tgatctcacc
     6061 gaccgagtgc ccggcgagca gatcggcgct gccggcgtcg gcgacggacg cggcctcggc
     6121 cagcgcggca tacgtcagca gcccggcggc gacgatgagc ggctgggcga ccgccgtgtc
     6181 cttgatcgtc tcctcgtcgg attcggtgcc gtgctcgacg acgtcgatgc ccgtggcgtc
     6241 gctgaactcc tcggcgttct cccgcagacc gggaagctcg agccagggcg tcaggaagcc
     6301 gggggtctgg gagccctgtc cggggcagac gatcgcaagc accatccaac cgtcccgcac
     6361 ttgaccgtca aaagcgcggc gaaaggtcac ggaatagacc gagtgctttt tggaggattc
     6421 ctacaagtcg ggtcaggatc gggccacgct ggcggtgccg ctgatgccgg cctcggccag
     6481 gcgccccagg cgaatggcga ggtggactgt gtcggcgtcg tgcgggtccc cgaggtcgta
     6541 gccggtcagt tcggcgatgc gcccgaggcg atagcggacg gtgttgggat ggacgaacag
     6601 gacgcgggcg gtcgcctcga gattgcggcc gccatcgagg tatgccgtgg ccgtgcggag
     6661 cagttcgccg ccgccggcct cggccagcgg ccgcacgatg cgggtcacca gggcgctgcg
     6721 ggccggcatg tcgccgatga gcgcgcgttc ggcgagcagt tcgctggccg gtgccggccg
     6781 gggcgcgtcc ggccaggccg ccgcggccgc ctgaccggag atcgcggccc gggcggaccg
     6841 gccggccgcg aacaggtgcg gcaccgtggg gccgaccacc agcgggccag cgccgaaaca
     6901 gtcggtgagc aaggcggcat cacgcagcgc atcggcggtc tcgccgatga gcacgatgag
     6961 gcgcccgccc tgcagggcgg cgagaacctg aagacggttg cgggcggcgg cgcggtgcag
     7021 ccggtcgagg accagagggg gatcgatcgc gacggcgtgg tcggcctccg gctgggcgcc
     7081 ggcgacgacc gcgacccggg tggtggcacc ccagccgaga gccgcggccc gcgactggat
     7141 cgagtcgtcg gcgtcgccgc gcaggatcgc atcgacgacc agcccctcga ggcgggcatc
     7201 ccacgcgccg cgctgctccg cggcgcgggc atagatgtgc gcggccccga aggccacatc
     7261 gcggctgtag cgcagcatgg cttccagcaa cgcgggcgct gtctccgggc cgacgagttc
     7321 ggccgcctgg acctcgacca cgtcgaccac ggagcggatc agatcaaggg tctgggcgag
     7381 gctgatcgag cgggtcaact ccggcggggc agcgtcgaag agggcctgcg ccgggacctg
     7441 aacggtggac tcgtcactga gccaggcgac gaagttctcg atcccgctgt gggccacgag
     7501 cgcaaccgac gcgcggtcgc gggccgacag cgcggcatac cacccgtggc gcgcctcgat
     7561 gtccgccacg gccgcgcgaa ccaggctcgc ggtgttcgcc gcgatctcgc ggcgagtttt
     7621 cgcgggcagg gcggcacgac gcggcatacc ccgtagccta tttgtacgga gcccacaatc
     7681 tgcggccacc tcggattcgg cggcgaatcg cggcccggtc ggcgtgagag gcgaaggccg
     7741 tgtccggatc ctgagattct tccgggccgg gtcttccctc gtcctgcgcg gcgaggtcta
     7801 tggtcgcggc atcccgatga cttcatccgc ttcggtcgcc gccagagatg acaacgttgt
     7861 caggccgagc tggaccagtc gtcctcggga ggtcaacgac gacgacttac caggagatcc
     7921 cgaccatgtc acgacgacga ttgcgccttc cggttgtcac cggcctcgtg ggcgccggca
     7981 ccacgctggg cctgctggcg acgagcgctc aggcgctccc gagtgcggtg ggcgacgagc
     8041 cctatcgccc ggcgatccac ttcacaccgg cgcgcaactg gatgaacgac ccgaacgggc
     8101 tcgtctacta caagggcacg tatcaccttt tcttccaaca caatccgcgt ggcgaccggt
     8161 ggggaaacat gagttggggg cacgcgacct cgacggacct ggtgcattgg cgcgagcaac
     8221 cgctggccat cccgcagacg ctgaacgccg ccggggaaag cgtggaggac atcttcagcg
     8281 gctcggtggt cgtcgacaag accaactcga gcggcttcgg cacggcgacg aaccccccgc
     8341 tggtcgcgat ctacaccagc gcctacaccg acaagcaccc gacgctcgcc ggcaagcagg
     8401 cgcagtccct cgcctacagc accgacggcg gtatgacgtg gaccaagtac gccggcaacc
     8461 ccgtgctcaa ccgggactcg gcgaacttcc gcgacccgaa ggtcttccgc tacagcggac
     8521 ccaagggcac ctactgggtg atggcggcgg tcgaggccca ggaacaccgg gtcctgctct
     8581 acaagtcgag ggatttgaag aactggacct acctgagcga cttcggtccg gcgaatgccg
     8641 ttggcgggca atgggaatgc ccggacctgt tccagctgca gatccaaggg acgacgacgc
     8701 gcaagtgggt catggtcgtc aacatcaacc ccggctccgt ggccggtggc agcggcgggc
     8761 agtacttcgt gggcaacttc gacggtgagc gtttcgtctc ggagtcgacg gtcagcgcgg
     8821 acacgctgcc gcccggcaca agcttcgcga gcttcgacga cgggacctat ggtgactgga
     8881 ccgtgtccaa cgagccggga aactgggcga atgggccgtg gggcgacgcc ccggccacgg
     8941 gcacgctgcc ggggcagaac ccggtcagcg ggttcgtcgg atcgggtctg gtcaatggct
     9001 tcctcgatca cgactggccg gtcggcacga tgcgctcacc ggagttcacg gtcgacgcgg
     9061 catacctcaa tttccttgtc ggaggaggca accacccgca tgtcgccggc actcagctca
     9121 gcaacgaccc gccggcgggc agcctgctgt gggacggctt cgagttcccc gacggcacca
     9181 cgctcgccga caccggctgg caggccaccg gcgacttcgc gacggagccg tggcgcaatc
     9241 cgtcgactgc cggtggcgat tactacatcg ggcgcaagcg gatcaacacc tgggagggcg
     9301 gcccgcgcgg cgacgacaac ctgggatcgc tgacctcgcc cgccttcgag atcacggccg
     9361 accacctgag catgctcgtc ggcggcgggc gccgcaccga cggcagcctc gaggtgcagc
     9421 tgctcgtggg cggccaggtc gtgcgcagcc tcaccggccc ggagggcggc gcgctgaact
     9481 ggcgcagctg ggacgtcgcg gacctgcgcg ggcagcaggc gcagctgcgg gtgtgggacc
     9541 aggccgccgg cggctggggt cacctgaccc tcgaccacgt cgtcatgggt gacaccccgg
     9601 cgatcccgcg ctcggacgag acggccgtca acctcgtcgt cgacggcaag gtggtggcga
     9661 gcgcgacggg tgcgaacagt gaatatctcg actggcactc cttcgacctg cggccctacg
     9721 ccgggcgcac ggcatacctc gagatcgtcg acaacaaccg gttcgggtgg ggacacatcc
     9781 tcgccgacga gttccggttc ggcgcgcagc cggccgcgac gcgtgccgag agctacgact
     9841 ggctcgactg gggccgggac tactacgccg ccgtctcgtg gaacgacgtg cccggcggca
     9901 agcgcgtgat gatcggctgg atgaacaact gggactacgc caacgacatc ccgacctccc
     9961 cgtggcgcag cgcaatgtcg ctgccgcgcc acgtctggct ggcgcagaca cccaagggcc
    10021 cgcggatcgt ctcccgggtc gtcccgcagg cggcagcctt ggatctgacc ggacaggcgt
    10081 atgccgctcc tgcccagtcg gtctcgggca gtcgcctgct cccgaagcgg ggaagtgtgg
    10141 cgcgcatcga cgccgtcctg cggccgggga cggcgaagcg tttcgggttg accgtgcacg
    10201 ctgccgctga tggctcggag aagacggtga tcggctacga caccacgacg ggccgcctct
    10261 tcgtcgaccg gaccgacagc gggaacgtcg ggttccaccc ggccttcccc tcggtcgagg
    10321 acgcccaggt ctccctggtc ggcggcaagc tcacgctgag cgtctatctc gaccgctcgt
    10381 ccgtggaact gttcgcccag ggcgggcgga cggccatcac cgaccaggtc ttcccctccg
    10441 cgggaagcaa ccggatcggg atgtgggccg agggcggcgc catgcgggtc gagagcctga
    10501 agatcactcc gctcaaggcc tcgatgttca ccgggcggta gatgatggag gcatgatcga
    10561 gccgatcacg ccgacgaccc cgaggccggt ccgccgggcc gtgctcactc agtggtggcg
    10621 cgacctggcg atcctgcact ggcctgtcgc tccgcacctg gtcgcgccgc tgttgccgcc
    10681 cgggacgacc cccgacaccc gaggaggggt cacctatgcg ggcctgatcg ccttcgagat
    10741 ggatcgggtc ggaccgggtt tcggtgtgcc tttcttcggc cggttcccgg agatcaacgt
    10801 ccgcctgtat tccgtcgacg cccagggtcg gcgcggagtc gtcttctgct cgctggaggc
    10861 ggcacgcctt gctccggtca tggtgggccg agcgctgggg ttgaactacc gctgggcgcg
    10921 catgcgtatg acgcgcacct ccggcggggt ctggaggcac agcagccgcc ggatcggcgg
    10981 ctcgcctggg cgctgccgtc tggccgtcga accccaccac gagatcgccg acgacgatct
    11041 gacccactgg ctcaccgacc gttggggcct gcacgtgcgt cgcgtcggac gcagcctcta
    11101 tgtgcccaac gagcacccgc catggcggat gcagacggcc tcactgctcg agctggacga
    11161 gaccctgatc accgcggcgg gcctgtcggt ggacccgggg gccccggtga gtgtgctgta
    11221 tgccagcggc gtgcccgtgc gcttcggcat gccccgtccg gtgtgaccgg ggcatcgtcg
    11281 aactcagtcg gtgccgaact ccatggccgc ggcgtcgagg gcgtcgtcgg tggaggggtc
    11341 ggtcgccggg ttgctggcga tctgcgccgc gccgccggcc tcgagttggc cgacgaggac
    11401 ctgctgaccg ccggtgagct ggccctgcgc ggcatacagc tccagtttgg agcgggagtc
    11461 ggcgatgtcg agattgcgca tggtcagctg gccgatgcgg tcgaccggcc cgaaagcggc
    11521 gtcctcgacg cgctccatcg agagcttgtc ggggtggtag ctgaagttgt cgccgcgggt
    11581 gtcgacgatc gtgtagtcgt cgccgcggcg caggcgcagg gtgacctggc cggagatcgc
    11641 ggacgccacc catcgctgga gggactcgcg cagcatgagc gattgcgggt cgagccagcg
    11701 gccttcatag agcaggcggc ccaggcggcg gccctcggcg tgatagttgg cgacggtgtc
    11761 ctcgttgtga acggcgttca ggagacgctc gtaggcgatg tgcagcaggg ccatgccggg
    11821 ggcttcgtag acgccgcgag acttggcctc gatgatgcgg ttctcgatct ggtcggacat
    11881 gcccaggccg tgccggccgc cgacggcatt ggcttggcgc acgagcgcca ccgggtcgag
    11941 atcggcgccg ttgatcgcca cggggcggcc ctggacgaag gtgatcgaca catcctcggt
    12001 agcgatcgac acggccgggt cccagaagcg cacacccatg atgggctcga cggtctcgag
    12061 cgaaacgtca aggtgctcaa gggttttggc ctcgtgcgtg gcgccccaga tattggcgtc
    12121 ggtggagtag gccttctcct ggctgtcgcg gtagggcaga tcccgctggc tgagccaggc
    12181 gctcatctcg tgccgcccgc cgagctcacc gacgaagtcg gcgtccagcc acggcttgta
    12241 gatccgcaag gccgggttgg cgagcaggcc gtagcggtag aagcgctcga tgtcgttgcc
    12301 cttgaaggtc gacccgtcac cccagatgct gacctggtcc tcgtgcatcg cgcgcacgag
    12361 cagggttccg gtgacggccc ggccgatcgg ggtggtgttg aagtaggcct tgccgccgga
    12421 gcggatgtgg aaggcgccgc acgcgatcgc ggacagtcct tcctcgacaa gggcgctcgt
    12481 gcagtcgacg agccgggaga gttcagcgcc gtactggccg gcgcggccgg ggatctggtc
    12541 gatctccggc tcgtcgtact gacccaggtc ggcggtgtag gtgcagggga tcgcgccctt
    12601 ctcgcgcatc caggccaccg ccaccgaggt gtcgagaccg ccggagaagg cgatgccgac
    12661 gcgctcgcct acgggaagtg aagtgaggac cttggacacg ggggccagcc taagtcccgc
    12721 ccggggcggc ccggaccgca cgtccagaaa gcgctccacc gatcgacgta tcacgagcac
    12781 gatcggcgcc tctgttggcc tacggaggtc gcacatcccg aggcggccac gagggaggaa
    12841 ggcatcacga tgcaacgcag gaagttcatt gcgagcgcgg ccctggcggc ggctatgacc
    12901 ggatcgctcg tcacggcgga cgcggcgacc gcggcccccg agtcgggtca gcgcgccggg
    12961 tggaccagca ccttcaacgg cagcaaggcg ggctggcaga acgtcgtcgg cacctgggtg
    13021 ctgaaaccgg gcaacctcta caacaccggg atcgcgggca agcggacatc ggtcaagcac
    13081 atcaacaact acaccaactt ctactacacg gtgcggatga agcggtgggg caactcgacc
    13141 ggcgtcgcgg ccaatgccat cgtcatccga ggcaatccgt cgtcacgcga cggatcgggc
    13201 tactggcggc cgagctactt gctccaatac agcaacaacg ggtactactc ggtctggcgg
    13261 atcaacgccg acgccactca gacggcgatc cagggctgga cgccagtgtc cggggtcatc
    13321 tcgaagtccg gctacaacaa gctcgaggtg tgggcctatg gcgattcgct cgacttccac
    13381 atcaatggcg ttcacctgtg gtcgggtttc gactcggcgc tgaccttcgg caacgtgggc
    13441 gtcagccagt acaccgagcc cggcaaatgg tctcagacgt ggctggactc ggcatcgctg
    13501 accgtcgcca cccgggtcgc accgcatgac cacggcgcca ccggcacccc ggtcaagggc
    13561 ggcacgatgg acatggcgcc ccgctgaacc ggccgcatac ccacggacac ctgatcggcc
    13621 cctgctcccc ggagtgaccc cgctccccgg cgtggggccg atctgcttcc acggcgatgc
    13681 cagactcgtg cgatggagat ccttgacgcc cttgcggcgg aagaagacca actcgccgcg
    13741 ctgcttgccg gcctgacgca ggagcagtgg ggggcggact cgctctgcgc gggctggagt
    13801 gtggccgacg tggtgctcca tctggctcag tgcgaggagg ccgtgatcgc cacgattcgc
    13861 ggcacccccg tcgactgggg cgggctcggc gacacggtcg gcgctgccat ggagtccgcg
    13921 gtgagcgccg agcgatcaga ttccggcgca acgatatgcg agcgctggga gcgcgcacgc
    13981 gggcaggcat tgaccgtgct ccgggccgca gacccggccc ggcgcgtcgc gtgggtgacg
    14041 aacaccctac ggccggcgac gctggcgacc actcgcctgg ccgagcactg ggcccacgcc
    14101 ctcgacatcg ccgaaccgct cggcctgccc tatccggaca cccaacggct gcgccacatc
    14161 ctgtggctgg cgcacgccac cctgccctac gcctactccg ccgccgggag cacggccccg
    14221 tcggtgcgga tcgaggcggt tgctccgaac gggcccgcgt gggtgctcgg cccggatgac
    14281 gccgacgtca tgctcagcgg tccggccggt gatctcgccc gtatcgccgc ccgccgcctg
    14341 gaccccggtg acagcgccgt ccggacctcg ggggcagccg ccgccgaggt gctcgtccgg
    14401 atccgcacct acgcgatctg acacgcgtgg tttggacggt cgataaggtc catgagcgtt
    14461 gcggatcgcc agcggggggc ggatacttgg cgctgagcag ggttccggcg gatcaggagg
    14521 cagccctgtg gaacctcttg tccccgtgat cgtcgcccac cgcggtgcgt gcggctatct
    14581 gcccgaacac acgctcgcgg catacgaact cggcatccgc ctgggggcgg acgccttcga
    14641 gatcgacgtc gtgccgacga cggacggcca cctcgtcgca cgccacgaga acgaactgag
    14701 tgtctcgacc gatgtcgcga cccgccgcgg attcgccgag cggcgcacca ccaagctgat
    14761 cgagggcgcc gcgcaggacg gctggttcgc cgaggatttc accctcgtcg aactgcggca
    14821 gttgctggcc cgtcagccgc tgcgcggcct gcgcagcacc gcgcacgatg atgcgtatgc
    14881 cgtggccagc cttcctgaga tcctcgccct ccgctcccgg ctggccgccg aactggcccg
    14941 cccggtcggg ctgaaactcg aggtgaaatc cccggcccac ttcgccgcga tcggccaccc
    15001 gatggcagac cggctgatca ccgacctcga tgccgcgggg atgctcggcg cggactcgcc
    15061 ggtggagatc atgaccttcg agccggcatt cctgcgctgc ctgcgcgccc gcggcgtcga
    15121 caacgacctg gtcctgctcg tcgaagaaga ccccacccgc ccggtcccgg gggagggcga
    15181 gaccaccttc gccgacctga ccacaccggc gagcctggcc gagctggcct cgctgatcac
    15241 cgggatcggc cccggccggc acgccctctt cagcggtttg cctgggggtc ggctagggga
    15301 gcccttgccc ctcttcgccg acgccaaggc ggccggcctg cgcctggatt gctggacctt
    15361 cggggccgag aacgctttcc tgccaaccga tttccgcatc ggtgacgacc cggccgccta
    15421 cggtgatatg gcgggtttcg tcgcggcata ccgggagcag ggcctcgaca cggtgatcac
    15481 cgaccacccc gacctggtca tccccacacc ccggtagccg ccggtcagcg aacagccgcc
    15541 ccagcctcag ctggggcggc tgttcgcgaa ctccggcgac ctgcgtcagg cgtcgccgcc
    15601 ggcgttgccg gaggctccgg cggtgacatc gaggaggcgg tacttctcgg ccgcctggcc
    15661 gggcaccgac gcatcgatct cgccgcgcgc ggcgagcgcc tgcagcgccc gcaccacgac
    15721 ggacggaccg tcgatgtgga agtagcgccg cgccgcaggg cgagtgtcgg agaagccgaa
    15781 gccgtccgcg ccgagggagt ggaagtcgct cggtacccac tgcgcgatct ggtcctgtac
    15841 cgcccgcatg tagtcggaca cggcgatcac cggacccggc atgcccgaaa gacgctgcgt
    15901 cacatacgga tcggcgacct gctgctcggg gtggaggaag cgcgtcctgt ccacctcgag
    15961 agcctcacga cgcagttcgt tccacgacgt caccgaccag acgtcggcgt tgacgcccca
    16021 gtcgtcgcgg agcagctgct gggcctccat cacccacggg acgcccacgc cggatgcgag
    16081 cagctgcacc gacgggttgt tctcccggcc ggaccggtcg gcgtccgcaa gccggtacat
    16141 gcccttgacg atgccctcga cgtcgacatt ctccggctcg gccggttgca ccatcggctc
    16201 gttgtagagg gtgaggtagt agatgacgtt cttctcgtcc ccgtcgccgg tttcgccgta
    16261 catctgctcc aggccgcgct tcatgatgtg cgcgatctcg tagccgaacg ccggatcgta
    16321 tgctcgcaca gccggattcg tggacgccag caggtgtgag tgcccgtccg cgtgctgcag
    16381 gccctcaccg gtcagggtcg tgcggccggc cgtcgcgccg agcaggaagc cgcgggacat
    16441 ctggtccgcc gccgcccaga tcgaatcggc cgtgcgctgg aaaccgaaca tcgaatagaa
    16501 gatgtacatc ggcaccatgg cctcgccatg ggtgctgtac gacgtgcccg ccgcggtgaa
    16561 cgccgccatc gaaccggcct cgttgatgcc ggtgtggacg atctggccgg cctcggattc
    16621 cttgtaggcc aacatgaggt cggcatccac ggagcggtag cgctgcccgt gcgggttgta
    16681 gatcttgatg gtcgggaaga aggagtccat cccgaaagtg cgcgcctcgt cgggaatgat
    16741 ggggaccacg cgttgcccga agtccttctc ccgcatcagg tccttgagca gccgcaccca
    16801 cgccatggtc gaggcgacct gctgcttgcc ggagcccttc ttgaccgcct tgtaggcgtc
    16861 gtcgccgggc agctgcagcg gggcgtagtc gaccttgcgc cgcggcagcc cgccgccgag
    16921 cttgccccgc atctcgaggg cgtactggat ggcctcgtcc tcggtgccgg ggtggtagta
    16981 gggcggctgg taggggtcgg cctcgagctg cgcgtccgtg atcgggatgt gcagcgtgtc
    17041 gcggaagccc ttgaggtcat cgagagtcag cttcttcatc tggtgggtcg cattgcgccc
    17101 ggcgaagtgc gacccgaggg tgtagccctt gatgg
//
LOCUS       DAOPJG010000055        17109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3534_length_17109_cov_7.637094, whole genome shotgun sequence.
ACCESSION   DAOPJG010000055 DAOPJG010000000
VERSION     DAOPJG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 17109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17109
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3534_length_17109_cov_7.637094"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..448
                     /locus_tag="PKU75_07075"
     CDS             251..448
                     /locus_tag="PKU75_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HPF80674.1"
                     /translation="MAGQEQVRPTRRGGDDDGPESDGGQVQARSENLDQDVDSILDEI
                     DGVLESNAEEFVKSFVQKGGQ"
     gene            445..1266
                     /gene="prcB"
                     /locus_tag="PKU75_07080"
     CDS             445..1266
                     /gene="prcB"
                     /locus_tag="PKU75_07080"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HPF80675.1"
                     /translation="MSGRGPGRLPDAFRAPGSSSFTEFLGVHAPDLLPSRRTLPKGPV
                     DAPHGTTIVTLTYAGGLLMAGDRRATMGNIIANRDMEKVFAADDFSVIGIAGTAGLAI
                     ELVKLFQVELEHYEKIEGALMSLEGKANRLATMIRGNLGMAMQGLTVVPVFGGYDLDA
                     SQGRIFSYDVTGGCYEEHDHHTIGSGSLFARGSLKKLWRPGLDVDQALHVAVEALYDA
                     ADDDSATGGPDLTRRIWPTVAYVEAHGVRFADEPAVHAAVEDVVAARTGNPGGAR"
     gene            1263..2072
                     /gene="prcA"
                     /locus_tag="PKU75_07085"
     CDS             1263..2072
                     /gene="prcA"
                     /locus_tag="PKU75_07085"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HPF80676.1"
                     /translation="MTQPPFYVSPEQVMKDRADFARKGIARGRSVIVVGYDKGIAFVA
                     ENRSRALHKVSEIYDRIGFAAVGKYNEFENLRVAGIRYADMRGYSYDRSDVTARGLAN
                     AYAQTLGTVFIQESKPFEVEIVVAEVCKTADEDQIYRLMYDGSVADEVGFVAMGGAAE
                     PIEAGLKEQWRAGMPLSEVLQMAVRLLGQDPAGGEARTLEPTALEVAVLDRTRPRRTF
                     RRINGDLLASLLSETDPTSDAPTDEASLGETHVSTNPQTQRPGAEATPDGD"
     gene            2092..3477
                     /gene="pafA"
                     /locus_tag="PKU75_07090"
     CDS             2092..3477
                     /gene="pafA"
                     /locus_tag="PKU75_07090"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HPF80677.1"
                     /translation="MKRRIYGIETEFGITCVAGGQRTLTPDEVARYLFRKVVSWGRSS
                     NVFLSNGSRLYLDVGSHPEYATAECDDIVDLVAHDKAGEQIINDLVADATERLAEDNV
                     AGEIYVFKNNTDSAGNSYGCHENYLIGRGGEFHDFSEVLIPFLVSRQLIAGAGKIVAQ
                     PKGSVFCLSQRADHIWEGVSSATTRSRPIINTRDEPHADAEHYRRLHVIVGDSNLSET
                     TTLLKVGSCDLILRMIEEGATLRDLTLDNAIRAIRDISSDLTGRRTVKLANGRQLSAL
                     DLQSEFFNKATEFAASKGYDDPVTTRVLDLWRRTLEAVESGDHSGIDTEIDWAIKHKL
                     LSAYAAKHGLDWSHPRLAQLDLAYHDIDPDRGLFNVLQRRGLAARVVDGARVEAAKDD
                     PPQTTRAKLRGEFIKVAKEHRRDYTVDWVHLKLNDQAQRTVLCKDPFLAKDHRVERLI
                     QGMTTGAIPQP"
     assembly_gap    3723..3822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3823..4733
                     /locus_tag="PKU75_07095"
     CDS             <3823..4733
                     /locus_tag="PKU75_07095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPF80678.1"
                     /translation="SAGGSASGSASASATAATDGSIDDIKVSGGDDKTAPKIDLAKKP
                     FGVAKYTSKVLKEGTGDATNAKSVAVVNYVLVNGTDGKTLDENFSTEAAGFDVGDESL
                     IQGLRAALTGVKKGSRVVAALTPTDTFGPQGNAQAGVAGTDTLIFVFDVADVVTKLDA
                     ATGKTVTPKAGLPKATWQEGKPATFEMPKSDPPKELVIQKLIEGEGAKVEKGDTVFVT
                     YTGALWRNGEVFDSSFKQSPTASFSFPVGAGKVVAGWDKGIEGQKVGSRLLLVVPPKD
                     GYGDAGQGDIKGDDTMVFVVDILGKY"
     gene            4766..5152
                     /locus_tag="PKU75_07100"
     CDS             4766..5152
                     /locus_tag="PKU75_07100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPF80679.1"
                     /translation="MPFDPNTERPEIDFPEGAPPADLVIEDIWEGDGAEATRGSTVLA
                     HYLGVAHSTGAEFDASWNRGAPLDFRLGVGQVIAGWDRGIVGMKVGGRRKLTIPAHLA
                     YGDRGAGGAIKPGETLIFVVDLVDVR"
     gene            5192..6112
                     /locus_tag="PKU75_07105"
     CDS             5192..6112
                     /locus_tag="PKU75_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPF80680.1"
                     /translation="MTAQSPDLPYPSSAEVPAGAIVVGIDGREEDANALDWAAAEAVS
                     LGAPVHVITALDIDLPLAGGGEAVPMALIEDALDDDATARLERACARVAERAPGVPVT
                     AYRSSGRAARVLLDASERARTVVLGSARGGNLERILLGATSTSVVALARCPVVVVGPV
                     LPEDGDIVVAFDDSPHSRAALWYAAATAERLRVPVRVVSGWFLEVVDGVVATTPGSPQ
                     YEKVKQRLHGSIDAALEPVIAAHPDVAFTAVVKQGKIVDIVLAESNDARLLVLGTRGR
                     GGIAGMLFGSVSRKVLQTAGVPVAVLSHGA"
     gene            complement(6126..7250)
                     /locus_tag="PKU75_07110"
     CDS             complement(6126..7250)
                     /locus_tag="PKU75_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HPF80681.1"
                     /translation="MHWRVGTVAAVERSWGRAVQYAVDLDSPGQAPGPRVRALGYADL
                     VGVLGAGDRVLLTTSALERGLGTGGLAFVVAAPDRLPPDPPAGPGHIVKARYTPQQQI
                     VLAVDEQESPHHGVLSSGSAAAGDLAGLPVLVADVHSALAPILVGVRSLAPQARVVHL
                     YTDGGALPAAFSMTLARLAELGWLTATGTVGQAFGGDFEAVTLHSGLLAARYVFAADL
                     VVVAQGPGNVGTGTPYGFTGLAAGEALNAVHVLGGRGFGALRISAADARERHHGISHH
                     SRTAFGRVVLGEASLIVPHLPGEFGALVAQQAGDLAAGARGQVTLISEPVDGLLEALR
                     ACPIPLSSMGRGLDADKEYFLAQAAAGRAVARTLGSAEAR"
     gene            7306..8292
                     /locus_tag="PKU75_07115"
     CDS             7306..8292
                     /locus_tag="PKU75_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPF80682.1"
                     /translation="MPPSNSPAAKTERLMNLVMTLLYTRRPLSRARIRETVEQYRLAP
                     TDEAFERMFERDKEELRELGIPLATEEISAGWEDEPGYRIHARDYALPQIRFDADELA
                     VLGLAARAWSHATLAAPAATALRKLRASGVEPDDEAFAFVEARLGTSEPAFGPLFDAV
                     LAQAPVRFGYARPGEEAATRHVQPWGLTSQSGHWYLTGLDTDRGEPRVFRLSRISGKV
                     TRAGKPGSYAVPADHDPVAMIRGRREGGRLDLVRLRVQSGSGNALRHRGIETAGDDGW
                     DKVELPYSDPGDIAHELASYGASLIVEEPPAVRDAVIAVLRAGADAHEEVGP"
     gene            8289..9245
                     /locus_tag="PKU75_07120"
     CDS             8289..9245
                     /locus_tag="PKU75_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPF80683.1"
                     /translation="MTPEGASGRLARLLSIVPWLVNRQGVDITHAAAELGVTEAQLRD
                     DLELLFMCGYGSMTDELIDVSTEGGRILISNADTIAQPLRLTRAEALTLMVGLRALLG
                     AEGVADSTVIERTLAKLDEAAASAGDVAGKVEAVLADDSAEHVREVVADALRRGRRLQ
                     LRYLVPARDESTDRAVDPMRLVSLDGRWYLEGWCHRAEDTRLFRLDRVEDIEVLDADG
                     TPPAGARARDLAAGTFVASQRDPLVTLELRPGWEWVGEYYPTESLARADGVTTVTLRV
                     GDREWVRRLALRAGGGVLVREPADLAAEVAGSARAALAAYGA"
     gene            9254..9562
                     /locus_tag="PKU75_07125"
     CDS             9254..9562
                     /locus_tag="PKU75_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80684.1"
                     /translation="MPWWASALVFLAILVGGGWWLWRHVRATWRSVRALNDQVSEASL
                     LIAELDRTASRLPTSGDVQLAVFTHPYDAHRERTRTRAIVADERRARREPKLPPWARR
                     "
     gene            9668..9973
                     /gene="tatA"
                     /locus_tag="PKU75_07130"
     CDS             9668..9973
                     /gene="tatA"
                     /locus_tag="PKU75_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HPF80685.1"
                     /translation="MPNLGPTELIIIALVIILLFGWKRLPDAARSLGRSARVFKSEVN
                     EMKAEDEARKRADGTMTTGTTTTGTTTTGSAGSTTVEGSALREDNSPRTDNQSGHTH"
     gene            9975..10814
                     /gene="tatC"
                     /locus_tag="PKU75_07135"
     CDS             9975..10814
                     /gene="tatC"
                     /locus_tag="PKU75_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPF80686.1"
                     /translation="MAPGLPGSAKRAARKANPEGRMSLIEHLIEFRQRLIKAALALVV
                     CSIIGWFLYDWVYAHLTAPMESVAAERGIEADKVALNFAGLTTAFSQRVNLSVWLGLI
                     MASPVIMYQLWAYIVPALTKKEKRVSMAFVAVTVPLFLIGCAFGYWVLPKAVEILLSF
                     TPVNAYNLPEAAMYFRFVTRLILVFGVTWVMPVALVGLIFAGIIPGNALFKQWRPALV
                     LIFIISAIVTPTPDPVTMFFMAGPLIVLYFIAAWIGILIGRRRAKNAPDWTKYADDEA
                     SPL"
     gene            10825..11772
                     /locus_tag="PKU75_07140"
     CDS             10825..11772
                     /locus_tag="PKU75_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HPF80687.1"
                     /translation="MPDAAAENPAGPRSGEISGTRVGLVVNPHAAAGSGNRIGARLMR
                     ELGAAGFDIVHLSGPTAGAALANARRSISEDRLDLLVVAGGDGMTHLGVNACAGTDIP
                     LAVVAVGTGNDIAGALGLPVRDVEASVAMIAAGRTRSVDAGRVDGDHHRPWFGGTLYA
                     GFDAVVNARANAWSWPRGQSRYNLAVVRELPVFSAIPYAVTIDGTRTETEAMLVIVAN
                     ASSYGGGMHVAPHALMDDGLFDVMILHKISRPEFLRVFPKVFSGTHVDHPAVQITRGR
                     RVLLEADGITAFADGEPFRRVPLRAEVVPGAVRVVVPAS"
     gene            11769..14510
                     /locus_tag="PKU75_07145"
     CDS             11769..14510
                     /locus_tag="PKU75_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPF80688.1"
                     /translation="MSHLAAFEASLSFPLDPFQRDACAAVEAGKGVLVAAPTGAGKTV
                     VGEFAVSLALTRGQKAFYTTPIKALSNQKYTDLVARHGSANVGLLTGDSSINGEAPIV
                     VMTTEVLRNMMYAGSSSLTGLGFVVMDEVHYLADRMRGAVWEEVIIHLPPSVQLISLS
                     ATVSNAEEFGDWLELVRGNHAVIVSEHRPVPLWQHMQVGSRMFDLFADSTAGPRRGGE
                     IPRLNPYLTDAIRQAEQRGYAEAAARRGARGRGGDPDRRRRPETAVRTAGGHATRTEI
                     IRDLDHQGLLPAITFIFSRVGCDAAVGQLLGNGIRLIPEEQGERNRRLVEERSASLAD
                     EDLGVLGYWDFVEGISRGFAAHHAGMLPTFREIVEELFTAGAIRAVFATETLALGINM
                     PARTVVLEKLVKFNGESHVDLSPAEYTQLTGRAGRRGIDIEGHALVVWRRGVDPVYVA
                     GLASTRTYPLRSSFRPSYNMAVNLVAQVGRSRASEVLQTSFAQFQADRSVVGLVQEGK
                     DRQAALEGYAEAMHCHLGDFREYAAIRHQISQLEKEGAKARSAARKAEAAVSLEALRV
                     GDIITIPAGRRAGLAVVIEPSRSYRGETSLPTVLTEDKHVKTLTLSDIPTAVTAMGSM
                     PVPRHFNAKSPKSRRDLATTLRIKAPRRPREVDPATAGRAAAGGEDARIAGLRRQLRA
                     HPCHACPDREEHARWSDRWWRLRRESDGLTRQVEARTNTVAKTFERICGLLTDLGYLV
                     DGGEAVSPDGERLRRLYSDRDLLTAECLRQGVWKRLDSSELAACVSMLVHEPRFGETD
                     PQPRMPTDGVAEAAARMLDIWASLEEREEAARLPHTPMPDAGLAWSVHRWAAGQRLDV
                     VLKDTDLAAGDFVRRCKQVVDLLGQIADAAPEPHLRTLARQATTSVMRGVVAADRLD"
     gene            complement(15312..>17109)
                     /locus_tag="PKU75_07150"
     CDS             complement(15312..>17109)
                     /locus_tag="PKU75_07150"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPF80689.1"
                     /translation="ALSGAVTTVASGAHAETPARAGATYLGTVLGGAFASMYPVDVAT
                     TSAYYYVVDPGRYRVERVVRATGKIDKWTKGGKGTGDGDMGAARAVAVDKSGAVYVAD
                     TPNNRMVKYSPDLQTELAKWGTKGTAPGQFTAVYGVAVGPGLDQNGAPAEVVYTVDGS
                     RVQVFTKTGALIKVFATGFNQPRQIEVNKVTGDVYVVNARERRIDVFSKAGNPLFSFG
                     SEGSGPGQFKGDPRGITLSRNGNIAFVTDDGNARIIAFSTTGTTRGKQLYAIGGGTSK
                     AFIDPRGVDVTPDGLLLVADEWDYSLKEYSFTSAGATLKRRLFGVRAPKTGANSPRGV
                     AVDAKGNLFVSDWWNQRIVRKDAATGAITTWGQRGIRGEVMSLNFQWAVAVQPGTGHV
                     FVANRESHEIKVFNNTGSQQLFVWGSRNSQPGNFTFPQGLAFAPDGTLWVADSGNSRV
                     QQFRVNVANKTATVLKAVGSAGTGVGQFGMPTGISVDADGTVYVADTLNNRIQAYRNG
                     SWSVVPVSGTRGFRVPWGVTAAPDGSLWVADTGNKRLVKMSTSGAFAYEVTGASAGTT
                     AFGGPFQVTFVNASTAYMSDTWGNRVIKLSVS"
BASE COUNT         2634 a         6065 c         5805 g         2505 t
ORIGIN      
        1 gaggctccaa caaggcggtc cgccgcagcg aatccgccgg gtcatcggat cgctccgtga
       61 aaacgtccca gccggcaacc cacctatccg cctggttctg tgaatcgaag tctaggacca
      121 tcgctcgtct cgggagcaat ggttggaacg gcatcctctc agattagccc ccggctttcg
      181 cggtaggcgc gccctcccgg catgcgggcg cttgtgacat aggctcggga gggacgaagg
      241 gagtttgccg atggcaggcc aggagcaggt gcgtccgacg cgccgtggcg gggacgacga
      301 cgggcccgag agcgacggcg ggcaggtcca ggcgcgcagc gagaatctcg accaggacgt
      361 cgacagcatc ctcgacgaga tcgacggggt gctggagtcc aatgccgagg agttcgtcaa
      421 gagcttcgtg cagaagggtg gccagtgagc ggtcgcggcc cgggccggct cccggacgcc
      481 ttccgcgcgc ccggatcctc ctctttcacc gagttcctcg gcgtgcacgc acccgacctg
      541 ctgccctccc ggcgcacgct gccgaagggc ccggtcgatg cgccccatgg cacgacgatc
      601 gtcaccttga cctacgccgg tggcctgctg atggccggtg accggcgcgc gacgatgggc
      661 aatatcatcg ccaaccgcga catggagaag gtcttcgccg ccgacgactt ctccgtcatc
      721 gggatcgccg gcacggcggg gctggcgatc gaactcgtga agctcttcca ggtcgagttg
      781 gagcactacg aaaagatcga gggcgccctg atgtccttgg agggcaaggc gaatcgtctc
      841 gcgacgatga tccgcggcaa cctcggcatg gccatgcaag gactgaccgt cgtcccggtc
      901 ttcggtggct acgacctgga cgcgagccag ggccggatct tctcgtacga cgtgaccggc
      961 gggtgctacg aggagcacga ccaccacacc atcggctccg gctcgctctt cgcccgcggc
     1021 tccctgaaga agctgtggcg acccggcctc gacgtcgacc aagccctgca cgtcgcggtc
     1081 gaggcgctct acgacgcggc cgacgacgac tccgccaccg gaggccccga cctgacccgg
     1141 cgcatctggc ccacggtcgc ctacgtcgag gcccacggcg tccggttcgc cgacgagccg
     1201 gccgtgcacg ccgctgtcga ggacgtcgtc gcagcccgca cgggcaaccc cggaggtgcg
     1261 cgatgaccca gccgccgttc tacgtctcgc ccgaacaggt gatgaaggac cgggccgact
     1321 ttgcacgcaa gggcatcgct cgcggccggt cggtcatcgt cgtcggatac gacaagggca
     1381 tcgcctttgt cgccgaaaac cgttctcgcg ccctgcacaa ggtctcggag atctatgacc
     1441 gcatcggctt cgccgccgtc ggcaagtaca acgagttcga gaacctgcgc gtcgccggca
     1501 tccgctacgc cgacatgcgc ggctactcct acgaccgatc cgacgtcacc gcccgtgggc
     1561 tcgccaacgc ctacgcccag accctgggca ccgtcttcat ccaggagtcg aagccgttcg
     1621 aggtcgagat cgtcgtcgcc gaggtctgca agaccgccga cgaggaccag atctatcgcc
     1681 tcatgtatga cggttcggtc gccgacgagg tgggcttcgt cgcgatgggc ggcgccgccg
     1741 agccgatcga ggccggcctg aaggagcagt ggcgagccgg tatgccgctg tcggaggtgc
     1801 tccagatggc cgtccgcctg ctggggcagg atcccgccgg gggtgaggct cgcaccctcg
     1861 agccgaccgc gctcgaagtg gcggtgctgg accgcacccg cccgcgacga accttccgcc
     1921 gcatcaatgg cgacctgctc gccagcctgc tgagcgagac ggacccgacc agcgacgctc
     1981 ccacggatga ggcaagtctc ggcgagacgc atgtctcgac caacccccag acgcagcgcc
     2041 cgggcgcgga ggcgacgccc gacggcgact gaggcgggcg gcatacccga catgaagcgg
     2101 cggatctacg gcatcgagac cgagttcggc atcacctgcg tcgccggcgg gcagcgaacg
     2161 ctgacgccgg acgaggtggc gcgctacctc ttccgcaagg tcgtctcgtg gggacgatcg
     2221 tcgaatgtct tcttgagcaa cggttctcgg ctctatctcg acgtcggctc gcacccggaa
     2281 tatgcgaccg ccgagtgcga cgacatcgtc gatctcgtcg cccacgacaa ggccggcgag
     2341 cagatcatca acgatctcgt cgccgacgcg accgagcggc tcgccgagga caatgtcgcc
     2401 ggcgagatct acgtcttcaa gaacaacacc gactcggccg gcaactccta tggctgccac
     2461 gagaactacc tcatcgggcg cgggggagag ttccacgact tctccgaggt gctcataccc
     2521 ttcctcgtca gccgccagct catcgccggc gccggcaaga tcgtcgccca acccaaaggc
     2581 tcggtcttct gcctcagcca gcgcgccgac cacatctggg agggcgtctc ctccgcgacc
     2641 acccggagcc ggcccatcat caacacccgc gacgaaccgc acgccgacgc cgagcactac
     2701 cgccgcctgc acgtcatcgt cggcgactcc aacctctcgg agaccaccac actgctcaag
     2761 gtcggcagct gcgacctcat cctgcggatg atcgaggaag gcgccacgct gcgcgatctg
     2821 acgctggaca acgcgatccg ggcgatccgc gacatcagca gcgacctgac cggtcgccgc
     2881 accgtcaaac tcgccaacgg ccgccagctc agcgcgctcg atctccagag tgaattcttc
     2941 aacaaggcaa cagagttcgc cgcgagcaag ggttatgacg atccggtcac cacgcgggtg
     3001 ctcgacctct ggcggcgcac gctcgaagcg gtcgagagcg gtgaccacag cggcatcgac
     3061 accgagatcg actgggcgat caagcacaaa ctcctgagcg cgtatgccgc caagcacgga
     3121 ctcgactgga gccatccccg cctggcgcag ctcgacctcg cctatcacga catcgacccc
     3181 gatcgtgggc tgttcaacgt cctgcagcgc cgcgggctcg cggcgcgggt ggtggacggg
     3241 gcacgcgtcg aggccgccaa ggacgacccg ccccagacga cccgggccaa gctgcggggc
     3301 gaattcatca aggtggccaa ggaacaccgg cgcgactaca cggtcgactg ggtgcacctg
     3361 aagctcaacg accaggccca gcggaccgtc ttgtgcaagg accccttcct cgccaaggac
     3421 caccgggtcg agcgcctcat ccagggcatg actaccggcg cgatcccgca gccctgagcc
     3481 cgcggcatac cgtccgcggc gggccaacgg ccaccgggct cggaactttc acccatgtga
     3541 ccgggttatg gtggggtgaa cccaggcggc ccgacgggcc gcggatcccc tccccgaaag
     3601 ctgttatcac cgtgcctgct gcccgtcgcg ccacccgcgc gctgaccgca ctgaccgccg
     3661 cgtccgcact caccctgggt ctggtggcct gcggatccgg cagctcgtcc tcgtccacct
     3721 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncctcggcc ggcggatcgg
     3841 cgagtggctc cgccagcgcc tcggccaccg cggcgaccga cggttccatc gacgacatca
     3901 aggtcagcgg cggcgacgac aagaccgccc ccaagatcga cctcgccaag aagcccttcg
     3961 gcgtcgcgaa gtacacctcc aaggtcctca aggaaggcac cggcgacgcc acgaacgcca
     4021 agagcgtcgc cgtcgtcaac tacgtgctcg tcaacggcac cgacggcaag accctcgacg
     4081 agaacttctc gaccgaggcg gcgggctttg acgtcggtga cgagagcctg atccagggtc
     4141 tgcgcgcggc cctgaccgga gtcaagaagg gctcccgtgt cgtcgccgcc ctcaccccga
     4201 ccgacacctt cggcccccag ggcaacgccc aggccggcgt cgcgggcaca gacacgctca
     4261 tcttcgtctt cgacgtcgct gacgtcgtga ccaagctcga cgccgcgacc ggcaagacgg
     4321 tcaccccgaa ggccggcctg cccaaggcga cctggcagga gggcaagccc gccaccttcg
     4381 agatgccgaa gtccgacccg cccaaggagt tggtgatcca gaagctcatc gagggcgagg
     4441 gcgccaaggt cgagaagggt gacaccgtct tcgtgaccta caccggggca ctgtggcgca
     4501 acggcgaggt ctttgactcc agcttcaagc agagccccac ggcgagcttc agcttccccg
     4561 tcggcgcagg caaggtcgtc gccggctggg acaagggcat cgagggtcag aaggtcggtt
     4621 cgcgcctgct gctcgtcgtg cccccgaagg acggctacgg cgacgccggc cagggcgaca
     4681 tcaagggcga cgacacgatg gtcttcgtcg tcgacatcct cggcaagtac tgatcgactc
     4741 cacgacaccc cgacaaggag cccacatgcc cttcgacccc aacaccgagc gtcccgagat
     4801 cgacttcccc gagggcgctc cgcccgctga cctcgtcatc gaggacatct gggaaggcga
     4861 cggcgcggag gcgacccgcg gctcgaccgt gctcgcgcac tacctcggcg tcgcccactc
     4921 caccggtgcg gagttcgacg cctcctggaa ccggggcgcg ccgctcgact tccgcctcgg
     4981 ggtcggccag gtcatcgccg gatgggaccg cggcatcgtc gggatgaagg tcggcggtcg
     5041 ccgcaagctg acgatccccg cgcacctcgc ctacggcgac cgcggcgccg gcggcgccat
     5101 caagccgggt gaaaccctca tcttcgtcgt agacctggtg gacgtgcggt aggccactga
     5161 ccagtccgca tccggggcct aggcttgggg tatgacagcg cagtcgccgg atctgcccta
     5221 cccgagttcg gccgaggtcc cggccggagc catcgtcgtc ggcatcgacg gccgcgagga
     5281 ggacgccaac gcgctggact gggccgccgc cgaggccgtc agtctcggtg caccggtgca
     5341 cgtgatcaca gccctggaca tcgacctgcc cctcgccggc ggtggtgagg ccgtgccgat
     5401 ggcgttgatc gaggatgcgc tcgacgacga cgcgaccgcc cgcctcgagc gggcctgcgc
     5461 gcgcgtggcc gagcgggcac cgggggtccc tgtcacggca taccgctcat cgggccgggc
     5521 cgcgcgggtc ctgctcgacg ccagcgagcg ggcgcggaca gtggtcctcg ggtcggcgcg
     5581 cggcgggaac ctcgagcgga ttctgctcgg cgccacgtcc acctcggtcg tcgccctcgc
     5641 ccggtgcccg gtcgtggtcg tgggaccggt gctgcccgag gacggcgata tcgtcgtcgc
     5701 cttcgacgac agcccgcaca gccgcgccgc gttgtggtat gccgcggcca cggccgagcg
     5761 cctgcgggtg ccggttcgcg tggtcagcgg atggttcttg gaagtcgtcg acggagtggt
     5821 ggccaccacc ccgggttcgc cgcaatacga gaaggtcaag cagcggctgc acgggagcat
     5881 cgacgcggcc ctcgagccgg tcatcgcggc ccaccccgat gtggcgttca ccgcggtcgt
     5941 gaagcaggga aagatcgtcg atatcgttct cgcagaatcg aacgacgcac gcctgctggt
     6001 gctcggcacc cgcggccgcg gtggcatcgc cgggatgctc ttcggctcgg tcagccgcaa
     6061 ggtcctgcaa acggccggcg tcccggtcgc cgtcctgagc cacggcgcgt aactcactgc
     6121 cggcatcagc gggcttcggc cgacccgagc gtgcgcgcca ccgctcggcc tgcggccgcc
     6181 tgcgcgagga aatactcctt gtcggcgtcc aggccgcggc ccatgctcga cagggggatc
     6241 gggcaggcgc gcagcgcctc gagcagcccg tcgaccggct cgctgatcaa ggtcacctga
     6301 ccgcgcgccc cggcagcaag atcgcccgcc tgctgcgcca ccagagcccc gaattcgccg
     6361 ggcaggtgcg gcacgatcag gctcgcctca ccgaggacga cccggccgaa tgcggtccgg
     6421 ctgtggtggg agatgccgtg gtggcgttcg cgggcgtccg ccgcgctgat gcgcagggcg
     6481 ccgaagccgc gtccgccgag gacgtgcacc gcgttgagag cctcgccggc cgccagaccc
     6541 gtgaagccgt atggcgttcc cgtgccgaca tttccgggcc cctgggcaac caccacgagg
     6601 tcagcggcga agacgtacct ggccgccagc agccccgaat gcagcgtcac cgcctcgaag
     6661 tcgccgccga aggcctgccc gaccgtcccc gtcgctgtca gccaacccag ttcggcgagc
     6721 cgcgcgagcg tcatcgagaa ggccgccggc agcgcaccgc cgtcggtgta gaggtggacg
     6781 acccgtgcct gcggcgccag cgaccggacc cccaccagga tcggcgccag cgccgagtgc
     6841 acgtcggcga cgagcaccgg cagtccggcc aggtccccgg cggcggccga cccggacgag
     6901 agcaccccgt ggtgcgggct ctcctgttcg tcgacggcca gcacgatctg ctgttgcggg
     6961 gtgtagcggg ccttgacgat atgacccggc cccgccggcg gatccggcgg caaccgatct
     7021 ggtgcggcga ccacgaacgc cagcccgccc gtcccgagcc cgcgctccag ggccgaggtg
     7081 gtgagcagca cccggtcgcc ggcacccaac acacccacca ggtcggcata cccgagagcg
     7141 cgcacgcgcg gccccggcgc ctggccgggg gagtcgaggt cgacggcata ctgcaccgcc
     7201 cgcccccagg accgctccac cgcggcgacc gtcccgactc gccagtgcat cacgggctca
     7261 gcgtaatgag ccagcacgcg atccctccgg tagcgtcgac ctgacatgcc acccagcaac
     7321 tccccggccg ccaagaccga gcggctgatg aatctcgtga tgacgctgct ctatacgcgc
     7381 cgtccgctct cgcgcgcccg gatccgcgag accgtcgagc aataccggct ggcgccgacc
     7441 gacgaggcct tcgagcggat gttcgagcgg gacaaggagg agctgcgcga actcggcatc
     7501 ccgctggcca ccgaggagat cagcgccggc tgggaggacg agcccggcta tcgcatccac
     7561 gcccgcgact acgcgctgcc gcagatccgc ttcgatgccg acgaactcgc cgtcctgggg
     7621 ctcgccgcac gcgcgtggtc ccacgcgacg ctcgccgccc ccgccgccac ggccctgcgc
     7681 aagctgcgcg ccagcggcgt cgaacccgac gacgaggcct tcgccttcgt cgaggcccgt
     7741 ctcggcacga gcgagccggc gttcgggccg ctcttcgacg ccgtgctcgc gcaggcgccg
     7801 gtgcgcttcg ggtatgcccg cccgggtgag gaggccgcga cccggcacgt ccaaccgtgg
     7861 ggcctgacgt cccagtccgg ccactggtat ctcaccgggc tggacaccga ccgcggcgag
     7921 ccgcgcgtct tccgcctctc gcggatctcc ggcaaggtca cccgggccgg caaacctggg
     7981 tcgtatgccg tgccggccga ccacgacccc gtcgccatga tccggggtcg ccgggagggc
     8041 ggccggctcg acctggtgcg gctgcgcgtg cagtcaggtt cgggaaatgc cttgcggcac
     8101 agggggatcg agacggcagg ggatgacggt tgggacaagg tcgagctgcc ctacagcgac
     8161 ccgggggata tcgctcacga gctggcctcc tatggcgcca gcctgatcgt cgaggaaccg
     8221 cccgccgtgc gcgacgccgt catcgcggtg ttgcgggccg gcgccgacgc ccacgaggag
     8281 gtcggcccat gacgcccgaa ggcgcgagcg gacggctggc tcgcctgctc tcgatcgtgc
     8341 cctggctcgt gaaccggcag ggtgtcgata tcacccacgc tgccgccgag ctgggcgtga
     8401 cggaggccca gctgcgcgac gacctcgagc tgctgttcat gtgcggctac gggtcgatga
     8461 ccgacgaact catcgacgtc tcgaccgagg gtgggcgcat cctcatctcc aacgccgaca
     8521 ccatcgccca gccgttgcgg ctgacccgcg ccgaggcgct caccctcatg gtggggctgc
     8581 gggcgcttct cggcgcggag ggagtggccg actccaccgt catcgaacgc accctggcca
     8641 agctcgacga agcggccgcg agcgccggcg acgtggcggg caaggtggag gccgtcctcg
     8701 ccgacgactc ggcggagcac gtgcgcgagg tcgtcgccga tgccctgcgc cggggccggc
     8761 gcctgcagct gcgctatctg gtgccggcgc gcgacgagag caccgatcgg gccgtggacc
     8821 cgatgcgcct ggtcagcctc gacggccgct ggtacctcga gggctggtgc caccgggccg
     8881 aggacacccg cctgttccgg ctcgaccggg tcgaggacat cgaggtcctc gacgccgacg
     8941 gcaccccgcc cgccggagcc cgggcgcgtg acctggccgc cgggaccttc gttgcgtccc
     9001 agcgcgatcc gctggtgacg ctggagttgc ggccgggctg ggagtgggtg ggggagtact
     9061 accccaccga gtccctcgcc cgcgccgacg gggtcacgac ggtgaccttg cgggtcgggg
     9121 accgtgagtg ggtgcgccgg ctggccctcc gcgccggcgg cggggtcctc gtgcgcgagc
     9181 cggcggacct ggccgccgag gtggcgggca gcgcgcgggc cgcgctcgcg gcatacggag
     9241 cgtgaggcac ggtatgccgt ggtgggcctc ggcgctggtc ttcctcgcca tcctcgtggg
     9301 gggcgggtgg tggctgtggc gccacgtccg tgcgacgtgg cggtccgtgc gcgccctgaa
     9361 cgaccaggtc agcgaggcgt cgctcctgat cgccgaactg gatcgcacgg cctcgcggct
     9421 gcccacgtca ggcgatgttc agctggcggt cttcacgcat ccatacgacg cccacaggga
     9481 acgcacaaga acgcgtgcca tcgttgctga cgagcgacga gcccggcgcg agccgaagct
     9541 cccgccctgg gctcgccggt gaacccaccc cacgaggtgt cctcgctgcg cttcggcact
     9601 tcgcgagggc cccggtcgcc ttgaccctag aatgaatccg acgacacacc aacgtgaaag
     9661 gcgcgtcatg cccaaccttg gtcccaccga gctgatcatc atcgccctgg tgatcatcct
     9721 gctcttcggg tggaagcgcc tgcccgacgc cgcccgctcc ctcgggcgct ccgcccgcgt
     9781 gttcaagtcc gaggtcaacg agatgaaggc cgaggacgag gcgcgcaagc gcgccgacgg
     9841 caccatgacc accggcacca cgaccaccgg cacgacgacc accggctccg ccggctcgac
     9901 gacggtcgag ggttcggcgc tgcgcgagga caacagcccg cggacggaca accagtccgg
     9961 tcatacgcac tgacgtggcg cccggcctgc cgggcagcgc caaacgggca gcccgcaagg
    10021 cgaaccccga ggggcgcatg tccctcatcg aacacctcat cgagttccgg caacggctga
    10081 tcaaggccgc cctggccctg gtggtctgct cgatcatcgg ctggttcctc tacgactggg
    10141 tctatgcgca cttgacggct ccgatggaat ccgttgcggc cgaacgcgga atcgaggccg
    10201 acaaggttgc cctcaacttc gccggtctga ccacggcctt cagccagcgc gtcaacctca
    10261 gtgtctggct cgggctgatc atggccagcc cggtgatcat gtaccagctg tgggcctata
    10321 tcgtcccggc cctgacgaag aaggagaagc gcgtctcgat ggccttcgtg gccgtcaccg
    10381 tgccgctctt cctcatcggc tgcgcgttcg gctactgggt gctgcccaag gccgtcgaga
    10441 tcctcctcag cttcacgccg gtcaacgcct acaacctgcc cgaagcggcg atgtacttcc
    10501 gcttcgtcac ccggctcatc ctcgtcttcg gcgtcacctg ggtgatgccg gtggcgctcg
    10561 tcgggctgat cttcgccggc atcatcccgg gcaatgccct cttcaagcag tggcgcccgg
    10621 cgctggtgct gatcttcatc atctcggcca tcgtgacccc gactcccgac ccggtcacga
    10681 tgttcttcat ggccggaccg ctcatcgtcc tctatttcat cgcggcctgg atcgggatcc
    10741 tcatcggccg gcgccgcgcc aagaacgccc ccgactggac gaaatacgcc gacgacgagg
    10801 cctcgcccct ataacctgct gcccgtgccc gacgctgcag ccgagaatcc cgccggccct
    10861 aggtccgggg agatctcggg cacccgcgtc ggcctggtcg tcaacccgca cgccgccgcc
    10921 gggagcggga accggatcgg ggcccggctg atgcgcgaac tgggtgccgc cggcttcgac
    10981 atcgtccatc tctccggccc gacggccgga gcggccctcg ccaacgcccg gcgatcgatc
    11041 tccgaggatc gcctcgacct gctcgtcgtc gccgggggcg acgggatgac gcacctgggc
    11101 gtcaacgcct gcgccggcac cgacataccc ctcgccgtcg tcgcggtggg cacgggcaac
    11161 gacatcgccg gcgccctggg cctacccgtg cgtgacgtcg aggcgtcggt ggcgatgatc
    11221 gccgccgggc gcacgcggtc cgtggacgcg gggcgcgtcg acggcgacca tcaccggccg
    11281 tggttcggcg gcacgctgta tgccgggttc gacgcggtcg tcaacgcgcg cgccaacgcg
    11341 tggtcctggc cgcgggggca gtcgcgctac aacctcgccg tcgtccgcga actcccggtc
    11401 ttcagcgcca tcccgtatgc cgtgacgatc gacggcaccc gcaccgaaac cgaggcgatg
    11461 ctggtcattg tggccaatgc ttcgtcatac ggtggcggga tgcacgtagc cccacacgcg
    11521 ctcatggacg acggactctt cgacgtgatg atcctccaca agatcagccg gcccgagttc
    11581 ctgcgcgtct tccccaaggt cttctccggc acccacgtcg accacccggc cgtccagatc
    11641 acccgcggcc ggcgcgtgct gctcgaagcg gacgggatca ccgccttcgc cgatggcgag
    11701 cccttccgcc gggtgccgct gcgggccgaa gtcgtgcccg gcgcggtgcg cgtcgtggtc
    11761 cccgcgtcat gagccatctc gcggccttcg aggcctcttt gagtttcccg ctcgatccct
    11821 tccagcggga cgcgtgcgcg gcggtggagg ccggcaaggg ggtgctcgtc gcggcgccga
    11881 ccggagcggg caagacggtg gtgggggagt tcgcggtctc cctggcgctc acgcgcgggc
    11941 agaaggcgtt ctacaccacg cccatcaagg ccctctccaa ccagaagtac accgatctgg
    12001 tcgcacggca cggcagcgcg aatgtcggtc tgctgacggg ggattcgtcg atcaacggcg
    12061 aggcgcccat cgtcgtgatg acgaccgagg tgctgcgcaa catgatgtat gccgggtcct
    12121 cctccctgac cgggctcgga ttcgtcgtca tggacgaggt gcactacctc gccgaccgga
    12181 tgcgcggggc ggtgtgggag gaggtcatca tccacctgcc gccgtcggtg cagctgatct
    12241 cgctctcggc caccgtcagc aacgccgagg aattcggcga ctggctcgaa ctcgtccggg
    12301 gcaaccacgc cgtgatcgtc tccgagcacc ggccggtgcc gctgtggcag cacatgcagg
    12361 tcgggtcccg gatgttcgac ctcttcgccg attcgaccgc gggtccgcgg cgcggcggcg
    12421 agatcccgcg gttgaacccc tatctcaccg acgcgatccg gcaggccgaa cagcgcgggt
    12481 atgccgaggc ggcggcccgc cggggtgcgc gcggccgcgg cggcgacccg gaccgccgcc
    12541 gccggcccga gacggccgtg cgcaccgccg ggggacacgc cacccgcacc gagatcatcc
    12601 gcgacctgga ccaccagggg ctgctgccgg cgatcacctt catcttcagt cgcgtcggct
    12661 gtgacgccgc cgtcggccag ctgctcggca acggcatccg gctgatcccc gaggagcagg
    12721 gcgagcgcaa ccggcggctg gtcgaggagc gctcggcgag cctggccgac gaggacctcg
    12781 gcgtcctcgg ctactgggac ttcgtcgagg ggatctcgcg tggtttcgcg gctcaccacg
    12841 ccgggatgct gccgaccttc cgcgagatcg tcgaggaact cttcaccgcc ggcgccatcc
    12901 gggccgtctt cgccacggag accctggcgc tcggcatcaa catgccggcg cgcacggtcg
    12961 tgctggagaa gctggtgaag ttcaacggcg agtcgcatgt cgacctctcg ccggccgaat
    13021 acacccagtt gaccggacgg gccggccgcc gcggcatcga catcgagggg cacgccctcg
    13081 tggtgtggcg gcgcggcgtc gacccggtct atgtcgctgg gctcgcttcg acgcgcacct
    13141 acccgctgcg ctcgagtttc cggccgtcct acaacatggc cgtcaatctc gttgcgcaag
    13201 tggggcgttc gcgggcgtcg gaggtgctgc aaacgtcgtt cgcgcagttc caggcggacc
    13261 ggtccgtcgt ggggttggtg caggagggca aggaccggca ggcggcgttg gagggctatg
    13321 ccgaggcgat gcactgtcat ctcggggact tccgggagta tgccgcgatc cgccaccaga
    13381 tctcccagct cgagaaggag ggggccaagg cgcggtcggc ggcccgcaag gccgaggcag
    13441 ccgtctcgct ggaggcgctg cgggtcggcg acatcatcac catccccgcc ggccggcgcg
    13501 ccgggcttgc cgtggtcatc gagccgtcca ggtcctatcg cggggagacc tcgctgccca
    13561 ccgtgttgac cgaggacaag cacgtcaaga ccctcacgct gagcgacatc cccaccgctg
    13621 tcacggcgat ggggtcgatg ccggtgcccc ggcacttcaa cgccaagtcg ccgaaatccc
    13681 gccgagacct ggcgacgacg ctgcgcatca aggcaccacg ccgcccgcgg gaggtcgacc
    13741 ccgccacggc cggccgcgcg gcggccgggg gtgaggacgc ccggatcgcc ggactgcgcc
    13801 gccagttgcg ggcccatccg tgccacgcct gcccggaccg tgaggagcac gcccgctggt
    13861 ccgatcgctg gtggcgactg cgccgggagt ccgacggcct gacccggcag gtggaggctc
    13921 gcaccaacac cgtcgccaag accttcgaac gcatctgcgg cctgctgacc gacctcggct
    13981 atctcgtcga cggcggcgag gcggtcagcc cggacgggga gcggctgcgg cggctctatt
    14041 cggaccggga cctgctgacg gccgagtgcc tgcgccaagg cgtctggaag cgcctggact
    14101 cctccgaact cgcggcgtgc gtgtcgatgc tcgtgcacga gccgcgcttc ggcgagaccg
    14161 atccgcagcc gcggatgccc accgacggcg tcgccgaggc ggccgcgcgg atgctcgaca
    14221 tctgggcgag cctggaggag cgggaggagg cggcgcggct gccgcatacc cccatgcccg
    14281 acgccggact ggcgtggtcg gtgcaccggt gggccgccgg ccagcgtctc gatgtcgtgc
    14341 tcaaggacac cgacctggct gccggcgact tcgtccgccg ctgcaagcag gtcgtcgacc
    14401 tgctcggcca gatcgccgac gccgcccccg aaccccacct gcgcaccctc gcccgccagg
    14461 ccaccacgtc cgtcatgcgc ggcgtcgtcg ccgccgaccg cctcgactga aaaccgttgg
    14521 ggcccagtaa cctctgctag ctgaggctag tcaaatcagc acagctagga cagtgtgctg
    14581 atttgactaa ggtctgctga gttgcgtcgc gggatcgccg tatgccgcgc gctgggtcgc
    14641 ttggagttgg cgggccaagg cgcgtgcctt ctccactttc tggcgcacga cagccaactg
    14701 gagcacttcg tcgggctgga tccgggcaaa ggcccagccg tcgagccgca ggtcgtcctc
    14761 gcggagcttc tccttcgcgg accagagcct tctgcgcgcg gggcgcttcc tgcggggtgt
    14821 aggtcgcctt cggccgaaca gcgcatgaag gcgttggcct tggactggtg actggccacc
    14881 actctccccc cgaaagaccg gagcagagct gccgcaagga gccggtgttt gttggcggcc
    14941 gcatcccgcg gcgtctcacc ctcccacggc gggacggggt cttcggggga ccacacggca
    15001 taccaccccc ggatcggtcg ccagatccgc ccggacttgc gcaattgggc gagttcgtag
    15061 ggtccgagac cgatcctgtt cgcctcggac gtagggacaa gatggtcgat ggccagctga
    15121 tgaagttcca tcgcgtcatc gtgtcggacg tcgcccggcc cggccagtct ccggtcgccg
    15181 agctgtggat aactcgaaac cagctggggc acagagcaac tcagcagagg ctagccaaat
    15241 cagcagaccg agcaaggtgt gctgattcga caagcctctg ctagcagagg ttacgtggcc
    15301 gaccccgcgg gtcagctcac cgagagcttg atgacccggt tgccccaggt gtcggacata
    15361 taggccgtcg aggcgttgac gaaggtgacc tggaaggggc cgccgaaggc ggtggtaccc
    15421 gccgaggcgc cggtgacctc gtaggcgaag gcgccggagg tgctcatctt gaccaggcgc
    15481 ttattgccgg tgtcggcgac ccagagcgag ccgtcggggg cggcggtgac gccccacggc
    15541 acccggaagc cccgggtccc cgagacggga accaccgacc acgaaccgtt gcggtaggcc
    15601 tggatccggt tgttgagggt gtcggcgacg tagacggtgc cgtcggcgtc gacggagatg
    15661 ccggtcggca tcccgaactg gcccacgccg gtgccggcgc tgccgactgc cttcaggacc
    15721 gtcgccgtct tgttggcgac gttgacgcgg aactgttgca cccggctgtt gcccgagtcc
    15781 gcgacccaca aggtgccgtc gggggcgaag gccaggccct gcgggaaggt gaaattgccg
    15841 ggctgggagt tgcgtgaacc ccagacgaac aactgctggc tgccggtgtt gttgaagacc
    15901 ttgatctcgt ggctctcgcg gttggcgacg aagacgtgac ccgtccccgg ttggaccgcc
    15961 accgcccact ggaagttcag cgacatgact tcgccgcgga tgccgcgctg gccccaggtc
    16021 gtgatcgcac cggtggccgc gtccttgcgg acgatgcgct ggttccacca gtcgctgacg
    16081 aagagattgc ccttggcgtc gacggccacg ccgcgcgggc tgttggcgcc ggtcttgggg
    16141 gcccgaaccc cgaacaaccg acgcttcagc gtggcaccag cggaagtgaa gctgtactcc
    16201 ttgagggagt aatcccactc gtcggcgacg aggagcaggc cgtcgggggt gacgtcgacg
    16261 ccgcgcgggt cgatgaaggc cttgctcgtg cccccgccga tggcgtagag ctgcttgccg
    16321 cgggtggtgc ccgtggtgga gaaggcgatg atccgggcgt tgccgtcatc ggtgacgaag
    16381 gcgatattgc cgttgcggct gagggtgatc ccgcgcgggt cgcccttgaa ctggcccggc
    16441 ccgctgccct ccgagccgaa ggagaagagc gggtttccgg ccttgctgaa gacgtcgatc
    16501 cgccgctcgc gggcgttgac gacatagaca tcgcccgtga ccttgttgac ctcgatctgg
    16561 cggggctggt tgaacccggt ggcgaagacc ttgatcaggg cgccggtctt ggtgaacacc
    16621 tgcacccggc tgccgtcgac ggtgtagacg acctcggcgg gggcgccgtt ctggtccagc
    16681 cccggaccga ccgcgacgcc atagacggcg gtgaactgcc cgggcgcggt gcctttggtg
    16741 ccccacttgg cgagttcggt ctgcaggtcc ggggagtact tcaccatgcg gttgttgggg
    16801 gtgtcggcga catagacggc gccggacttg tcgaccgcga ccgcgcgggc ggcgcccatg
    16861 tcgccgtcgc cggtgccctt gccccccttg gtccacttgt cgatcttgcc ggtggcacgc
    16921 acgacgcgct cgacgcgata gcggcccggg tcgacgacgt agtaataggc gctcgtcgtg
    16981 gcaacgtcga cgggatacat cgacgcgaat gccccgccga ggaccgtgcc gaggtaggtc
    17041 gcgccggccc gggcgggggt ctcggcgtgg gcgccggacg cgacggtggt gacggccccc
    17101 gagagggcg
//
LOCUS       DAOPJG010000056        16798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3629_length_16798_cov_7.947082, whole genome shotgun sequence.
ACCESSION   DAOPJG010000056 DAOPJG010000000
VERSION     DAOPJG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 16798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16798
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_16798_cov_7.947082"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2958)
                     /locus_tag="PKU75_07155"
     CDS             complement(<1..2958)
                     /locus_tag="PKU75_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPF80690.1"
                     /translation="MSDSQWPPDEVPEQPAPSRPARRAMSPRRRRGPLVPTLVVLGAL
                     AFLATGLAEVWTDVLWYRSVNFSGVFTTTLLTRIGLGAAAFVLVGGLVWSSLYLAYRH
                     RPFYVPQLGANESLEQYRDAIEPVRKVALFAIPGVVGALAATAASGQWRTYLAWVNQE
                     PFGKTDPHFHKDIGFFVFTVPWLQALIGVLTLALGAALAAAAFTHYIYGGLQLPGRGS
                     STRAAMVHVGVLAALLALVRAGSYLVDRYALATKDAPLMTGIRYTDAHAVLPTKIILA
                     VAALIVALLFVAAIFTHSWRLPMIGVALLVVTSVVVGSVYPAIIQSVKVNPSEKSLEA
                     PYLQKNIEATRAAYGLDGVQVQPYSAATDATAGKLRNDAATIPGIRLVDPIVVPPTFK
                     QLQALRTYYTFPDALDIDRYRIDGKETDIVIGAREISLDGVPAAQRGWLNDHTFYTHG
                     YGLVAAYGNQRKPGGEPVFAVGDLPPANTLGEFEPRIYFGEQSPNYSVVGANPGEAPR
                     ELDYPDSKEEEVKNTYAGTGGVPIGSVLRQAAYAVKYREVNFLLSNAVGSASKILDHR
                     RPLERVQRVAPWLALDGNPYPAIVDKRVVWIVDGYTTSANYPYSQMENLTTATSDSTT
                     ARATSVAALRAGQVNYVRNSVKATVDAYDGTVRLYAWDEADPILKTWMKAYPGTVSKT
                     SEISGDLMSHLRYPEDLLKIQRLVLSRYHVTRADALFSGNDNWRVPNDPAQEDRSVFQ
                     PPYYLTLKMPGQEAPSFSLTTPFMPSGDRQVLSGFLAVDADAGSQAGTKADTYGTLRL
                     LELPRDSNVKGPGQVQNDINSSNTSSPGFSTFPLSVYLNNNRQQGSRVTLGNLLTLPV
                     GGGLLYVQPVYVSASAESAFPLSRVTVVAFGDKLAWSDTLERALNLLFEGESGAPVED
                     PGTPTTPTQPQSGEEALAAALAEMKTAFEDGKKALAEGDFAAYGKAQERLQQALDKAI
                     AAAPGGAQLDLTPS"
     gene            3017..3553
                     /locus_tag="PKU75_07160"
     CDS             3017..3553
                     /locus_tag="PKU75_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPA1309 family protein"
                     /protein_id="HPF80691.1"
                     /translation="MTDTSTPKRDPLQLVAIETERYVSADGWDQPTRLFALVPTHELA
                     SSAPGLDVGGVDADHLSAVEQEGFAPDGDLPRALETLAWPPAVRGCALAVERLVVPPA
                     AERDLPSDPAAAVQQLQRHPDRQDVRLLVAVLRDGPSVCLIRQRSHDADNLVGTGPDL
                     APGLVAALLASLRDDVPE"
     gene            complement(3561..4652)
                     /locus_tag="PKU75_07165"
     CDS             complement(3561..4652)
                     /locus_tag="PKU75_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPF80692.1"
                     /translation="MKVAERHTAPPWQPDPGRLSPRVARLLWAAFVGITLAAVIALIP
                     LPYAILRPGPATNTLGQVAGKPLVAIEGATTYPTEGALDFTTVSVVGGPGRRVNAWDL
                     LGAAIDPSAEIFDEDQLFPKGATSEEVKEENALEMTTSQHEAAAVALRSLGKSVPNSY
                     VVASVTKGSGADGVLLAGDVITGVDGEAVASMVDIQRLVRTHKAGEDVTVSVRRDGAA
                     KDLIVRTTASGDTVVLGVRLRMDYDFPVAVTIDAGDVGGPSAGTMFALAVRDLLTPGA
                     MTGGKKIAGTGTIDDAGTVGPIGGIRQKIVGAKEAGAGYFLAPAANCPELAGHVPDDI
                     TVVKIATFDEAVSAVDGIAAGKTGLPSCS"
     gene            complement(4649..5092)
                     /locus_tag="PKU75_07170"
     CDS             complement(4649..5092)
                     /locus_tag="PKU75_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPF80693.1"
                     /translation="MAQPDRVHDVVRLCAISAEPLSVDAALAAIADDGCGGQVVFIGR
                     VRDHDHARAVTALEYEAHPGALAALQRVCRAVADAHDVRAVAAVHRVGALAIGDIAVI
                     TAVAAPHRDAAFAAGRELIDQLKATTPIWKHQLFGDGESEWVGLP"
     gene            5274..6746
                     /locus_tag="PKU75_07175"
     CDS             5274..6746
                     /locus_tag="PKU75_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPF80694.1"
                     /translation="MPDDIERPENDPQESGAGLPPEMQEALRRLTGGADLPPEVLQQF
                     EQMGLGAIPVDQFEGMMSQVQAMFNAPDEGPVSPTIATDVARKLIAAEGDAAMGEREG
                     ALAREAAHVADMWLGEVTAIEAPGLHGVAWSRSEWIEDTMPVWQELVSPVASGVTKSL
                     GRAMTAQFSEGGFEAMSSMLPAGTNPAQAIAQMGPLLERAGSSMFSSQLGQGLAALAG
                     DTLTGTEVSLPLTSGTQLALVPANIAAFAEGLEIDLEQVWLYLAVREAARLRLFHGVP
                     WLPQQILVAVQDYARDIAIDTEGIEAALDGIDATDPETLQATLADKLFAPKPTPAQQA
                     ALARLETWLALVEGWVDVVTDRAAGAHLPHSAALGETVRRRRALGGPAEKTFSGLVGL
                     QLRPRRLRDAANLFAALEDRGGAQLRDSAWGHPDVAPMGADLDDVLGYVDRRSNPAAS
                     DDLDAALAAILGETPPGDEPPPGEPSSGDDPSEAPRDDSP"
     gene            6743..7351
                     /locus_tag="PKU75_07180"
     CDS             6743..7351
                     /locus_tag="PKU75_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPF80695.1"
                     /translation="MNAGQPQGRQVAGIAPTPVRHLYDDALALLLSWRAPNQTQEQLR
                     RGFLSALAGDPMAVSKSGPPAHLTASCLVLSPDGGSVLLHLHGRAGMWLQFGGHLEMG
                     DDSIWAAARREAQEESGLDGLEPLPAPIDLDRHALVGNFGHCTEHLDIRFLAVTPSGR
                     MPRASDESTDIAWFPVDALPSNIAADLPRLIDVGRARLAAGA"
     gene            complement(7383..7931)
                     /locus_tag="PKU75_07185"
     CDS             complement(7383..7931)
                     /locus_tag="PKU75_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPF80696.1"
                     /translation="MTPDLPSPAPDVEIRRSARRRRTVSARREGERIIVMMPAGLSAA
                     EEQRWVETMVGKLERSARRTPRGRSDAELTDRARRLSAAYLEGRAQPTSVRWVGNQER
                     RWGSCTPSTGQIRLSDRLAGMPEYVVDYVLVHELAHLLEHGHGPAFWALVDRYPRTER
                     ARGFLDGISLAAGLDMTAPDEE"
     gene            8069..8230
                     /locus_tag="PKU75_07190"
     CDS             8069..8230
                     /locus_tag="PKU75_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HPF80697.1"
                     /translation="MSEEIYKGEFYCVKCKEKREAEGKIVVTENGRRMAKGVCPVCGT
                     NLNRILAKA"
     gene            8359..9204
                     /locus_tag="PKU75_07195"
     CDS             8359..9204
                     /locus_tag="PKU75_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80698.1"
                     /translation="MATWPRLAEQFRPLVRLDGSVQFGLDPGSGVRLCGLTEAEVGWL
                     RNLDGGADPVRSGTRAGVDPARLQELLHLLGQANLLGEHGGDARGETARVWVEGRLRL
                     ADRIAALLREGDIGELARLTADDPIPAYRRGPALVVLVTDQPTGARDAQRWRARGIPH
                     LPVALGPDTATVGPLVPARGGPCLGCVDLARADRDPSWSLIAAQARVGADQPAGRADV
                     SLVAIAAGLAARVATGFLDGMPPTGLALDVAAPWPSVRQHRWRTHPLCATCAEPAGAP
                     VTIGA"
     gene            9201..10535
                     /locus_tag="PKU75_07200"
     CDS             9201..10535
                     /locus_tag="PKU75_07200"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HPF80699.1"
                     /translation="MNELPRRSAKRTAKLVSLPLGMAGRGAIGIGKRVGGRPAEAVAA
                     EFQARTAEQLFAVLGELKGGAMKVGQAMSVMEAALPEEWAAPYRATLTKLQEAAPPLP
                     PEQVHAVLAAELGPRWRTAKFESFDDIPSAAASIGQVHRAVWRDGRDVAVKIQYPGAR
                     EALLSDINQLSRVARLATSFIPGLDIKPIMAELRARMSEELDYFMEAEHQRVFSDAFR
                     GDPDFAIPEVVHATPAVLVNEWLAGVPLSRIIAEGDQETRNAAAQLYLEFLLAGPTRA
                     GQLHADPHPGNFRITPDGRLGILDFGAVNRLPGGLPLALGRLLTLALAGEADALVAGL
                     RQEGFIRESVKVDADELLSYLMMFIEPLTYETYRFDRAWLRSITLRMNDIRRPEWAVG
                     MKFNLPPEYLLIHRVWLGGIGVLCQIGAELAPREALSRYLPEFDLSAIPPGR"
     gene            complement(10594..11166)
                     /locus_tag="PKU75_07205"
     CDS             complement(10594..11166)
                     /locus_tag="PKU75_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HPF80700.1"
                     /translation="MSQLRIAQDEHADEVLSTDPFGLLVGMLLDQQFPMERAFAGPAK
                     ILDRFGTLDPAAIARADPETFADLCATPPAIHRYGRSMAARVQALAEVVVASYGGDAS
                     TVWTSAKDTTDLLARLKALPGFGDQKARIFAALLGKQLGVTPDGWREAIGPYAEPGSF
                     RSVADVVDPDSLQKVRDFKKAAKAKATANT"
     gene            complement(11177..11326)
                     /locus_tag="PKU75_07210"
     CDS             complement(11177..11326)
                     /locus_tag="PKU75_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80701.1"
                     /translation="MRPSTPKLGRTRLFLGTCGAAGYLIRLVAKPELADKLFEDLVAS
                     LRRSE"
     gene            complement(11432..12463)
                     /gene="ilvC"
                     /locus_tag="PKU75_07215"
     CDS             complement(11432..12463)
                     /gene="ilvC"
                     /locus_tag="PKU75_07215"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPF80702.1"
                     /translation="MAEMFYDDDADLSLIQGRKVAVIGYGSQGHAHSLCLRDSGVDVR
                     VGLAEGSKSRAKAEDEGLRVLNVADAVKEADFVVILTPDQVQRHVWADHIAPNLNKGA
                     TILFGHGFNIRFGYITPPSDSDVIMVAPKGPGHIVRREFVDGRGVPVLVAVEQDASGQ
                     ALEMAKSYAKALGGLRAGGIKTTFTEECETDLFGEQSVLCGGLSQLIMYGFETLTEAG
                     YQPEVAYFECLHEVKLIVDLMVEGGIAKQRWSVSDTAEYGDYVSGPRVIDPHVKENMQ
                     AVLADITSGAFAKRFIDDQDNGGAEFTALREKGAQHPIEKTGKELRKLMAWLDTEQDA
                     DYVEGSAAR"
     gene            complement(12579..13091)
                     /gene="ilvN"
                     /locus_tag="PKU75_07220"
     CDS             complement(12579..13091)
                     /gene="ilvN"
                     /locus_tag="PKU75_07220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPF80703.1"
                     /translation="MSTHTLSVLVEDKPGVLARISALFSRRGFNIISLAVGPTEHPDV
                     SRMTVVVDVEELPLEQVTKQLNKLVEVLKVVELDRDSSIQRQIVLVKIRADAHTRSQV
                     LEIVQLFRAKVVDVTPEAISVEATGNVDKIHDLLELLEPYGVKELVQSGVVALGRGSR
                     SITDRALRTA"
     gene            complement(13113..15023)
                     /locus_tag="PKU75_07225"
     CDS             complement(13113..15023)
                     /locus_tag="PKU75_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HPF80704.1"
                     /translation="MTTTDGVAARSATPSPATMAHKAQPQSNRVEVTGAQSLILALEA
                     VGADTVFGLPGGAILPAYDPLLDSTTVRHILVRHEQGAGHAAEGYAAATGKVGVCMAT
                     SGPGATNLVTPIADAYMDSVPIVAITGQVSSRAIGTDAFQEADIRGITMPITKHNYLV
                     TNADDIPRVIAEAFHIAATGRPGPVLVDIAKDALQSKTTFVWPPRVSLPGYHPVIKPH
                     AKQIREAARLIQAARRPVLYVGGGVIRAHAAEDLAAFVAATGIPVVTTLMARGAFPDS
                     HQLHLGMPGMHGSVAAVTALQKSDLLITLGARFDDRVTGELKSFAPDAKVIHADIDPA
                     EISKNRVADVPIVGDAKAIIADLTTAVTSGEYQADPAAYQAWRETTIGWKSTFPVGYS
                     APSSDALMPQYVIERLGAIAGPEAVYVPGVGQHQMWAAQFIRYERPMAFANSGGLGTM
                     GYSVPAAMGAKVAEPDRTVWAIDGDGCFQMTNQELATCVINNIPIKIAVINNSSLGMV
                     RQWQTLFYNERYSNTDLHTSVGMRVPDFVKLAEAYGCVGLRCESPDDVDATITKAMEI
                     NDVPVLIDFVVERDAMVWPMVAAGVSNDLIQIAKDTAPQWDREESEAMEQDLDADHDG
                     IPSGSHRPETTA"
     gene            complement(15542..>16798)
                     /gene="ilvD"
                     /locus_tag="PKU75_07230"
     CDS             complement(15542..>16798)
                     /gene="ilvD"
                     /locus_tag="PKU75_07230"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPF80705.1"
                     /translation="CSGMFTANSMNCLTEAIGLSLPGNGSTLATHAARKDLFLRAGSL
                     IVELCRRYYDGDDDSVLPRNVASHDAFTNAFALDVAMGGSTNTVLHLLAAAREADLDF
                     RMADIDAISRRVPCLAKVAPNSPKYHMEDVHRAGGIPAILGELDRAGLLQRDVHAVHS
                     PSLSKWLGEWDIRGGSATEAALELFHAAPGGVRTTEPFSTENRWASVDVDQAEGCIRS
                     VDHAYTVDGGLAVLTGNIAPDGCVVKTAGVPESVWRFTGTARVFESQDDAVAGILDKS
                     VTAGDVVVIRYEGPKGGPGMQEMLYPTSFLKGRGLGQQCALITDGRFSGGTSGLSIGH
                     ISPEAASGGAIALIEDGDTVEIDIPGRSIDLAVSEEILAERRAKQEARERPYTPVNRD
                     RQVSAALRAYASMATSAADGAVRIVP"
BASE COUNT         2418 a         5661 c         6082 g         2637 t
ORIGIN      
        1 ggacggggtc aggtcgagct gcgcgccccc gggggcggcc gcgatcgcct tgtccagggc
       61 ctgctgcagc cgctcctgcg ccttgccgta tgccgcgaag tcgccctcgg ccagcgcttt
      121 cttgccgtcc tcgaacgccg tcttcatctc ggcaagcgcc gcggccagcg cctcttcgcc
      181 gctttgtggc tgcgtcggtg tcgtcggcgt gccgggatcc tcgaccgggg ctcccgactc
      241 gccctcgaag agcaggttca gcgcccgctc cagcgtgtcg gaccaggcca gcttgtcccc
      301 gaacgcgacc acggtgacgc ggctcagggg gaaggcggat tcggcgctgg cactgacata
      361 caccggctgc acatacagca gcccgccgcc gacgggcagg gtcagcaggt tgcccaaggt
      421 gacccgggag ccctgctggc ggttgttgtt cagatagacg ctgagcggga aggtcgagaa
      481 tcccggcgag ctcgtgttgg aggagttgat gtcgttctgg acctgcccgg gccccttgac
      541 gttggagtcg cgcggcagct cgagcagccg caaggtgccg taggtgtcgg ccttcgtgcc
      601 cgcctggctc ccggcgtcgg cgtccacggc caggaagccc gagagcacct gccggtcgcc
      661 ggagggcatg aagggggtcg tcaacgagaa gctgggcgcc tcctgcccgg gcatcttcaa
      721 agtgaggtag tagggcggct ggaagacact gcggtcctcc tgggcgggat cgttgggcac
      781 gcgccagttg tcatttccgc tgaagagcgc gtcggcgcgg gtgacgtgat agcgggagag
      841 cacgagccgc tggatcttca gcagatcctc cggataacgc aggtgcgaca tcaggtcgcc
      901 gctgatctcc gaggtcttcg agacggtgcc gggataggcc ttcatccagg tcttcaggat
      961 cgggtcggcc tcgtcccagg catacagccg caccgtgccg tcgtaggcgt cgacggtggc
     1021 cttgaccgag ttgcgcacgt agttgacctg cccggcgcgc agcgcggcga cgctggtcgc
     1081 gcgcgcggtc gtcgagtccg acgtggccgt ggtgaggttc tccatctgcg aataggggta
     1141 gttcgccgag gtggtgtagc cgtcgacgat ccacacgacc cgcttgtcca cgatggcggg
     1201 ataggggttg ccgtccaggg ccagccacgg cgcgacccgc tgcacccgtt ccagcggacg
     1261 gcggtggtcg aggatcttcg aggcggagcc gaccgcgttg gagaggagga agttcacctc
     1321 gcggtatttc acggcatacg ccgcctgtcg cagcaccgag ccgatcggca ccccaccggt
     1381 gccggcgtag gtgttcttga cctcctcctc cttggagtcg gggtagtcga gttcgcgcgg
     1441 cgcctcgccg ggattggcgc cgacgacgga gtagttcggt gattgctcac cgaagtagat
     1501 gcgcggttcg aactccccga gcgtgttggc cggcggcagg tcgccgaccg cgaagaccgg
     1561 ctcaccaccc ggcttgcgct ggttgccgta tgccgcgacg aggccgtagc cgtgggtgta
     1621 gaaggtgtgg tcgttcagcc agccgcgctg cgccgccggc acgccgtcga gggagatctc
     1681 tcgagcgccg atcacgatgt cggtctcctt gccgtcgatc cggtaacggt cgatgtccag
     1741 agcgtccggg aaggtgtagt aggtgcgcag cgcctgcaac tgcttgaagg tcggcggcac
     1801 gacgatcggg tcgacgagcc ggatgccggg gatcgtggca gcgtcattgc gcagcttgcc
     1861 tgcggtggcg tcggtcgcgg ccgaataggg ctgcacctgc accccgtcca ggccgtatgc
     1921 cgcccgggtc gcctcgatgt tcttctgcag gtagggcgcc tcgagggatt tctccgacgg
     1981 gttgaccttg accgactgga tgatcgccgg atagacgctg ccgacgacga cggaggtgac
     2041 gaccagcagg gcgacgccga tcatcggcag ccgccacgag tgcgtgaaga tcgccgcgac
     2101 gaagagcagg gcgacgatca gggccgccac ggcgaggatg atcttggtcg gcaggaccgc
     2161 atgggcgtcg gtgtacctga tgcccgtcat cagcggggcg tccttggtgg ccagggcgta
     2221 gcggtccacg agataagaac ccgcgcggac gagcgccagc agggccgcga gcacgccgac
     2281 gtgcaccatg gcggcccgcg tcgacgatcc ccggcccggc agttggagtc cgccgtagat
     2341 gtagtgggtg aacgcggcgg cggccagggc ggcgccgagc gcgagcgtga ggacgccgat
     2401 gagcgcctgc agccacggca cggtgaagac gaagaagccg atgtccttgt ggaagtgcgg
     2461 gtcggtcttg ccgaagggtt cctggttcac ccacgccaga taggtgcgcc attggccgga
     2521 cgcggccgtc gcggccagcg cgccgaccac cccggggatg gcgaagaggg cgaccttgcg
     2581 gaccggttcg atggcgtcgc gatactgctc gaggctctcg ttggccccca gctgtgggac
     2641 gtagaagggc cggtgccggt aggcgaggta gaggctggac cagaccaagc caccgacgag
     2701 cacgaacgcg gcggcgccca gcccgatccg cgtgagcagg gtggtggtga agacaccgga
     2761 gaagttcacc gagcggtacc acaggacgtc ggtccagacc tcggccagcc cggtggcgag
     2821 gaacgccagc gcgccgagca ccaccagggt cggcaccaag ggtccgcgcc ggcgccgcgg
     2881 cgacatcgcg cgacgggccg gacgactcgg cgccggctgc tcggggacct cgtcgggggg
     2941 ccactgcgaa tcgctcaccg ggtcaaccta cataccgtgt ctgggtatga cgcgcccgga
     3001 caggcaggat tggggcgtga ccgacacttc gaccccgaaa cgtgacccgc tgcagctggt
     3061 cgccatcgaa actgagcggt atgtcagcgc cgacggctgg gatcagccga cgcgcctgtt
     3121 cgcgctggtg ccgacccatg agttggcgag cagcgcgccc ggtctcgacg tcggtggcgt
     3181 cgacgccgat catctgtccg cggtcgagca ggaggggttc gccccggacg gcgacctgcc
     3241 gcgggcgctc gagacgctgg cgtggccgcc ggcagtgcgc ggctgcgccc tggccgtcga
     3301 acgcctggtc gtgcccccgg cggcagagcg cgacctgcca tccgatccgg cggcggccgt
     3361 gcagcagttg cagcgccacc ccgaccgtca ggacgtgcgc ctcctcgtcg ccgtgctgcg
     3421 cgacggcccg tcggtgtgcc tgatccgcca gcgcagccat gacgcggaca acctcgtcgg
     3481 caccggtccc gatctcgccc ccggcctcgt cgccgcgctg ctggcgagcc tgcgggacga
     3541 cgtgccggag tgagtcgccc tcaggagcag ctgggcaggc cggtcttgcc ggccgcgatg
     3601 ccgtcgacgg cgctgacggc ctcgtcgaag gtcgcgatct tgacgaccgt gatgtcgtcg
     3661 gggacgtggc cggcgagctc cgggcagttc gcggccgggg cgaggaagta gcccgcgccg
     3721 gcctccttgg cgccgacgat cttctgccgg atgccgccga tcgggccgac cgtgccggcg
     3781 tcgtcgatgg tcccggtgcc ggcgatcttc ttgccgccgg tcatcgcgcc gggcgtgagc
     3841 aggtcgcgaa cggcgagggc gaacatggtg ccggcggacg gccccccgac gtcgccggca
     3901 tcgatcgtga ccgcgaccgg gaagtcgtag tccatccgca gccgcacgcc cagcacgacg
     3961 gtgtcgccgg aagccgtcgt ccggacaata aggtccttgg ccgcgccgtc gcggcgcacg
     4021 gacaccgtga cgtcttcgcc cgccttgtgc gtgcgcacca gccgctggat gtccaccatg
     4081 gacgccaccg cctccccgtc gacgccggtg atcacatcgc cggccagcag caccccgtcc
     4141 gcgcccgagc ccttcgtcac gctcgcgacg acgtagctgt tgggcacgct cttgccgagg
     4201 ctgcgcagcg cgacggccgc cgcctcgtgc tggctggtcg tcatctccag ggcgttctcc
     4261 tccttgacct cttccgaggt cgcccccttg gggaagagct ggtcctcgtc gaagatctcg
     4321 gcgctggggt cgatggcggc gccgagcaga tcccaggcat tgacccggcg gccggggcca
     4381 ccgacgacgg agacggtggt gaaatcgagg gccccctccg tggggtaggt cgtggcgccc
     4441 tcgatggcga ccaggggctt gcccgccacc tggccgaggg tgttggtggc cgggccgggc
     4501 cgcaggatgg cgtagggcag cgggatgagg gcgatcaccg ccgcaagggt gatcccgacg
     4561 aacgcggccc acagcagccg ggccacccgg ggtgagaggc ggccggggtc gggctgccac
     4621 ggcggcgccg tatgccgttc cgccaccttc acggcaggcc cacccattcg ctctcgccgt
     4681 cgccgaaaag ctgatgcttc cagatcgggg tcgtcgcctt gagctggtcg atcagttcgc
     4741 ggcccgcggc gaaggcggcg tcgcgatggg gcgcggcgac cgcggtgatg acggcgatgt
     4801 cgccgatcgc gagggcaccg acgcggtgga ctgcggcgac ggcccggacg tcgtgggcgt
     4861 cggcgaccgc gcggcatacc cgctgcaggg ccgcgagcgc tccgggatgc gcctcatact
     4921 ccagcgcggt gacggcgcgg gcgtggtcgt gatcgcggac ccgtccgatg aacaccacct
     4981 gccccccgca cccgtcgtcg gcgatcgccg cgagcgcggc gtcgacggag agcggctccg
     5041 ccgagatcgc gcacaggcgc accacgtcgt ggacgcggtc gggctgcgcc atgtgtactc
     5101 cttcggtggg ccggcgggaa ctggaacggc tcccgcatgg ttgtcttggg tgacacattc
     5161 tcacccaggc gcattcagga caccccacaa agttccgcgc tcgcaagttt gctccggtac
     5221 tttgcggggt cccggccacc tgggtttcgt ttcgcgtctt gaggagtagt gccgtgcccg
     5281 acgacatcga gcgtcccgag aacgacccgc aggagtccgg agccgggttg ccgccggaga
     5341 tgcaggaggc gttgcgccgc ctcaccggcg gtgccgacct gccgccggag gtgttgcagc
     5401 agttcgagca gatggggctc ggcgcgatcc cagtggacca gttcgagggg atgatgtcgc
     5461 aggtccaggc gatgttcaac gctcccgacg agggcccggt ctcccccacg atcgccaccg
     5521 acgtggcccg caaactcatt gcggcagaag gcgacgcggc catgggcgaa cgcgagggcg
     5581 ccctggcgcg cgaggccgcg catgtggccg acatgtggct cggcgaggtc accgcgatcg
     5641 aggcccccgg cctgcacggc gtcgcgtggt cgcgctcgga gtggatcgag gacacgatgc
     5701 ccgtctggca ggagctggtc tccccggtcg cctcgggcgt gacgaagtct ctcggccggg
     5761 cgatgacggc gcagttctcc gagggcgggt tcgaggcgat gtcgtcgatg ctgccggcgg
     5821 gcaccaaccc ggcgcaggcg atcgcccaga tgggcccgct gctcgaacgg gccgggtcct
     5881 cgatgttctc ctcgcagctc ggccagggtc tggccgcact ggccggcgac acgctgaccg
     5941 gcaccgaggt gtcgctgccg ctgaccagcg gcacccagct ggcactggtc ccggccaata
     6001 tcgccgcctt cgccgagggg ctggagatcg atctcgagca ggtgtggcta tatctcgcgg
     6061 tgcgggaggc ggcccggctg cgcctcttcc acggggtgcc gtggctgccg cagcagatcc
     6121 tcgtggccgt ccaggactac gcccgcgata tcgccatcga caccgaaggc atcgaggccg
     6181 cgctcgacgg catcgatgcc accgacccgg agaccttgca ggccacgctc gcggacaaac
     6241 tcttcgcccc caagccgacg ccggcccagc aggcagcgct ggcccgcctc gagacctggc
     6301 tggcgctggt cgaaggctgg gtcgacgtcg tcaccgaccg ggccgccggt gcgcacctgc
     6361 cgcacagcgc cgcgctcggc gagacggtgc ggcgccggcg ggccctcggc gggccggccg
     6421 agaagacctt ctccgggctg gtcggcctcc agttgcggcc gcgccggctg cgcgatgccg
     6481 ccaatctctt cgccgccctg gaggatcgtg gtggcgcgca actgcgggac tcggcgtggg
     6541 gtcaccccga cgtcgcgccg atgggagcgg atctcgacga cgtgctcggg tatgtcgacc
     6601 ggcgcagcaa tcccgccgcg tccgacgatc tggatgccgc gctggcggcg atcctcgggg
     6661 agaccccgcc gggcgacgag ccgccgccgg gcgagccgtc gtcgggcgac gaccccagcg
     6721 aggcccctcg agatgattcc ccgtgaacgc cggtcagccg cagggccggc aggtcgccgg
     6781 gatcgcgccg acgccggtgc gccacctgta tgacgacgcg ttggccctcc tgctctcctg
     6841 gcgggccccg aaccagacgc aggagcagtt gcgccgcggg ttcctgtcgg cgctcgcggg
     6901 cgacccgatg gcggtgtcga agtccgggcc gccggcccac ctgaccgcgt cctgcctggt
     6961 cttgagcccg gacggcgggt cggtgctgct gcacctgcac ggccgggccg ggatgtggct
     7021 gcagttcggg ggtcacctcg aaatgggcga tgacagcatc tgggcggccg cgcggcggga
     7081 ggcacaggag gaatcgggcc tggacggcct cgaaccgctg ccggccccga tcgacctgga
     7141 ccggcacgcg ctcgtcggca atttcggcca ctgcaccgaa cacctcgaca tccgtttcct
     7201 cgcggtcacc ccgtccgggc ggatgcctcg ggccagcgac gagtccaccg atatcgcgtg
     7261 gttccccgtt gacgccctcc cgtcgaatat cgccgcggat ctcccccggc tcatcgacgt
     7321 gggccgggcc cggttggccg ccggcgcctg agcccgcgca ggtccgctct gcttcggggt
     7381 ggctattcct cgtccggggc ggtcatgtcg agcccggctg ccagggagat cccgtcgagg
     7441 aagccccgcg cacgttcggt gcgcgggtaa cggtccacca gcgcccagaa ggccgggccg
     7501 tggccgtgct cgagcagatg ggccagctcg tgcacgagga cgtagtcgac gacatactcc
     7561 ggcatcccgg cgagtcggtc gctgagccgg atctgccctg tcgacggggt gcacgacccc
     7621 cagcggcgct cctggttgcc gacccagcgc acggacgtgg gctgggcccg gccctccagg
     7681 tatgcggcgg agagccggcg cgcccggtcg gtcagctcgg cgtcgctgcg gccgcgcggg
     7741 gtccggcggg cggagcgttc cagcttgccg accatggtct cgacccaccg ctgctcctcg
     7801 gctgcgctga ggccggccgg catcatgacg atgatccgct ccccctcccg gcgggcgctg
     7861 acggtgcgcc ggcggcgggc gctgcgccgg atctcgacgt ccggagccgg gctgggtagg
     7921 tcgggggtca cggtcgctga gcgtaaaccc cgttcgttta cgcgtggcgc caatcccact
     7981 ctcacgctga gcgtgatcta gggtgggcac ggccccgggg cacccacccg ggtagagagc
     8041 ctgactcaga accacataag gagcatccat gtccgaggag atctacaagg gcgagttcta
     8101 ctgcgtcaag tgcaaggaga agcgcgaggc ggagggcaag atcgtcgtca ccgagaacgg
     8161 ccggcggatg gccaagggcg tctgcccggt ctgcggcacc aacctcaacc ggattctcgc
     8221 caaggcctga aagctgcacc gctcccccgg aggggcgttg tccacaccct gtggacaacg
     8281 cccctccgcc gcgtccggcc tctgccaggg tggtgacacg ggttccggcc cccggcgggg
     8341 cggcgcgaag ggagtgtcat ggccacgtgg ccacgattgg ccgagcagtt ccggccgctg
     8401 gtgcggctgg acggcagcgt gcagttcggg ctggaccccg gctccggggt gcggttatgc
     8461 gggctgaccg aggccgaagt gggctggttg cgcaacctcg acgggggtgc cgacccggtc
     8521 cgctcgggca cgcgagccgg ggtcgacccc gcgcgcctgc aagaactgct ccacctgctc
     8581 ggccaggcca acctcctcgg cgagcacgga ggcgatgccc ggggcgaaac cgcccgggtg
     8641 tgggtcgagg gccggttgcg gctcgccgac cggatcgccg ccctgctgcg tgagggcgat
     8701 atcggcgaac tcgcccgcct caccgcggac gaccccatcc cggcataccg gcgaggaccc
     8761 gccctcgtcg tcctcgtcac cgaccagccc accggtgccc gcgacgcaca gcggtggcgg
     8821 gcgcgcggca taccacacct gcccgtcgcc ctcggcccgg acacggcgac ggtgggcccc
     8881 ctcgtgcccg cccgcggcgg cccctgcctc ggctgcgtcg acctggcgcg ggccgaccgc
     8941 gacccgtcgt ggagcctgat cgctgctcag gcccgcgtcg gggccgacca gccggccggg
     9001 cgggcggacg tctcgctcgt ggccatcgcg gcggggctgg ccgcgcgtgt cgccaccggt
     9061 ttcctcgacg gtatgccgcc caccgggctc gccctcgacg tcgccgcacc gtggccgagc
     9121 gtgcgccagc accggtggcg cacccacccg ctgtgcgcca cgtgcgccga gccggcgggt
     9181 gcgcctgtga caataggcgc gtgaacgaac ttccccggcg cagcgccaaa cgcaccgcta
     9241 agctcgtctc cttgccattg gggatggccg gccggggggc gatcgggatc ggcaaacggg
     9301 tcggcgggcg gcccgcagag gcggtcgcgg ccgagttcca ggcccggacg gcggagcaac
     9361 tgttcgccgt gctcggcgaa ctcaagggtg gcgccatgaa ggtcgggcag gccatgtccg
     9421 tcatggaggc ggcgctgccc gaggagtggg cggcgcccta tcgggcgacg ttgaccaagc
     9481 tgcaggaggc ggcgccgccg ctgccgccgg agcaggtgca cgcggtcctg gccgccgagc
     9541 tcggaccccg gtggcgcacg gcgaaattcg agtcgttcga cgacataccg tcggcggccg
     9601 cgtcgatcgg ccaggtgcac cgggcggtct ggcgtgacgg gcgggacgtg gcggtcaaga
     9661 tccagtaccc cggggcgcgg gaggcgctgc tcagcgacat caaccagctg tcgcgggtgg
     9721 cccggctggc gacgagcttc atccccggcc tcgacatcaa gccgatcatg gccgagttgc
     9781 gggcccggat gagcgaggaa ctcgactact tcatggaggc cgagcatcag cgggtcttct
     9841 ccgacgcatt ccggggcgac ccggacttcg cgatccccga ggtcgtgcac gcgacccccg
     9901 ccgtgctggt caacgagtgg ctcgccggtg tgccgttgtc gcggatcatc gccgagggcg
     9961 accaggagac ccgcaacgcc gccgctcagc tctatctgga gttcctgctc gccgggccga
    10021 cgcgggcggg gcaactgcac gccgacccgc atcccggcaa cttccggatc acgcccgacg
    10081 ggcggctcgg catcctcgac ttcggcgccg tcaaccgcct cccgggcggc ctcccgctcg
    10141 cgctgggacg gctgctcacc ctggccctgg ccggcgaggc ggacgccctc gtcgccgggt
    10201 tgcggcagga gggcttcatc agggagtcgg tgaaggtcga cgccgacgag ctgctgtcct
    10261 atctgatgat gttcatcgaa ccgctgacgt atgagaccta ccgtttcgac cgggcctggc
    10321 tgcgctcgat cacattgcgc atgaacgaca ttcgccgccc ggagtgggcc gtgggaatga
    10381 agttcaacct gccgcccgag tatctgctca tccaccgcgt ctggctcggc ggcatcggcg
    10441 tcctctgcca gatcggtgcc gaactcgccc cccgcgaagc cctcagccgc tatctgcccg
    10501 agttcgacct ctcggccatc ccgcccggcc gctgaccccc gtcgagatgt cgattcgagg
    10561 gcgtcgagat gtcgactcga ccccgtcgag atgtcacgtg tttgccgtgg ccttggcctt
    10621 cgcggccttc ttgaagtccc gcaccttctg cagcgaatcg ggatcgacga cgtcggcgac
    10681 ggagcggaag gagcccggct cggcatacgg tccgatcgcc tcgcgccagc cgtccggcgt
    10741 gaccccgagt tgcttgccga ggagggcagc gaagatgcgc gccttctggt cgccgaaccc
    10801 cgggagcgcc ttgaggcggg cgaggaggtc cgtggtgtcc ttcgccgagg tccagacggt
    10861 gctcgcgtcg ccgccgtatg aggcgaccac cacctccgcg agcgcctgca cccgggcggc
    10921 catcgaccgc ccgtagcggt ggatcgcggg cggggtggcg cacaggtcgg cgaaggtctc
    10981 gggatccgcg cgggcaatgg ccgccgggtc gagcgtgccg aaccggtcca ggatcttcgc
    11041 cggcccggcg aaggcgcgtt ccatcgggaa ctgctggtcc agcagcatgc ccacgaggag
    11101 cccgaagggg tcggtgctca agacctcgtc ggcgtgctcg tcctgggcga tccgcagctg
    11161 tgacatgggc gcaaggttat tcggagcgtc gcagggatgc gacgaggtcc tcgaaaagct
    11221 tgtccgcgag ttcgggcttg gccacgagac gaatcaggta ccccgccgcg ccgcaggtgc
    11281 ccaggaagag tcgggtgcgc cccagctttg gtgtggatgg acgcacgatt cggatgctac
    11341 tcctcgctca gagacgccgt tacccattgg ttgtgggggg tgcacacccc cacaaccaat
    11401 gggtaaccga gtcgttgtgc gcgggcgagc gtcagcgggc ggcggagccc tcgacgtagt
    11461 cggcgtcctg ctcggtgtcg agccaggcca tcagcttgcg cagttccttg ccggtcttct
    11521 cgatcgggtg ctgggcgccc ttctcgcgca acgccgtgaa ctcggcgccg ccgttgtcct
    11581 ggtcgtcgat gaagcgcttg gcgaaggcgc cggaggtgat gtcggcgaga acggcctgca
    11641 tgttctcctt gacgtgcggg tcgatgacgc gggggccgga gacgtagtcg ccgtattcgg
    11701 cggtgtcgga gaccgaccag cgctgcttgg cgatgccgcc ctcgaccatc aggtcgacga
    11761 tcagcttgac ctcgtgcagg cactcgaagt aggcgacctc gggctggtag ccggcctcgg
    11821 tcagggtctc gaagccgtac atgatcagtt gcgacagccc accgcacagc accgactgct
    11881 cgccgaacag gtcggtctcg cattcctcgg tgaaggtggt cttgatgccg ccggcacgca
    11941 ggccgccgag ggccttcgcg taggacttgg ccatctcgag ggcctgcccg gacgcgtcct
    12001 gctcgacggc gacgagcacc ggcaccccgc gcccgtcgac gaactcgcgg cgcacgatgt
    12061 ggccggggcc cttgggggcg accatgatga cgtcggagtc gctcggcggg gtgatatagc
    12121 cgaagcggat gttgaacccg tgaccgaaga ggatcgtcgc gcccttgttg aggttgggtg
    12181 cgatgtggtc ggcccacacg tgccgctgca cctggtcggg cgtgaggatg acgacgaagt
    12241 cggcttcctt gaccgcgtcg gcgacgttca gcacgcgcag cccttcgtcc tcggccttgg
    12301 cgcgggactt gctgccctcg gcgaggccga cgcgcacgtc gactccgctg tcgcgcaggc
    12361 agaggctgtg ggcgtggccc tggctgccgt aaccgatgac cgcgaccttg cggccctgga
    12421 tcagcgacag gtcggcgtcg tcgtcgtaga acatctcggc catcgtggcc aactccttgt
    12481 gaggtggggt gcagggaact gtgggaaagc gtatgccgcg ggctcaggcc cgtcggcccc
    12541 ggtccgcccg ccgggaaacg ggatgtgacg tgtggcggct atgccgtgcg cagggcgcgg
    12601 tcggtgatcg agcgggagcc gcggccgagg gccacgacgc cggactggac gagttccttg
    12661 acgccatagg gctcgagcaa ctcgagcagg tcgtggatct tgtcgacatt gcccgtcgcc
    12721 tcgaccgaga tcgcctcggg ggtgacgtcg acgactttgg cgcggaacag ttggacgatc
    12781 tcgaggacct ggctgcgggt gtgggcgtcg gcgcggatct tgaccaggac gatctggcgc
    12841 tggatggagc tgtcgcggtc cagctcgacg accttgagca cctcgacgag cttgttcagc
    12901 tgtttggtca cctgctccag cggcaactcc tcgacatcga cgacgacggt catgcgggag
    12961 acgtcggggt gctccgtcgg gccgacggcg agggagatga tgttgaaccc gcggcgggag
    13021 aagagcgccg agatccgcgc gagcacgccg ggcttgtcct cgacgagcac ggagagggta
    13081 tgcgttgaca tgttggggat tcctcgtctg gatcaggcgg tggtctcggg acggtggctg
    13141 ccggacggta tgccgtcgtg gtcggcgtcg aggtcctgtt ccatcgcctc ggactcctct
    13201 cgatcccatt gcggcgcagt gtctttggcg atctggatca gatcgttgct gacgccggcg
    13261 gcgaccatcg gccacaccat ggcgtcgcgt tccacgacga agtcgatcag cacgggcacg
    13321 tcgttgatct ccatcgcctt ggtgatggtg gcgtcgacgt cgtccgggct ctcgcagcgc
    13381 aggccgacac agccatacgc ctcggccagc ttgacgaagt cgggcacccg catgccgacg
    13441 gaggtgtgca ggtcggtgtt ggagtagcgc tcgttgtaga agagcgtctg ccactggcgc
    13501 accatgccga ggctggagtt gttgatcacc gcgatcttga tcgggatgtt gttgatgacg
    13561 caggtcgcca gctcctgatt ggtcatctgg aagcagccgt cgccgtcgat cgcccacacc
    13621 gtgcggtcgg gttcggcgac cttggcaccc atcgccgccg gcaccgaata gcccatcgtc
    13681 cccaaaccgc cggagttggc gaacgccatc ggccgctcgt atctgatgaa ctgcgccgcc
    13741 cacatctggt gctggccgac gccgggcacg tagacggcct cggggccggc gatcgcaccc
    13801 agccgttcga tgacgtattg cggcatgagc gcgtcgctgc tcggcgccga atatcccacg
    13861 gggaaagtgg atttccagcc gatcgtcgtc tcgcgccagg cctggtatgc cgccgggtcg
    13921 gcctggtatt cgccgctggt gaccgcggtg gtcaggtcgg cgatgatcgc cttggcgtcc
    13981 ccgacgatcg gcacgtcggc gacccggttc ttggagatct cggccgggtc gatgtcggcg
    14041 tgaatcacct tggcgtccgg ggcgaacgac ttcaactcgc ccgtgacgcg gtcgtcgaaa
    14101 cgagcgccga gggtgatgag cagatccgac ttctgcagcg ccgtcacggc cgccaccgac
    14161 ccgtgcatgc ccggcatgcc gaggtgcagt tggtggctgt cggggaaggc accgcgggcc
    14221 atcagcgtgg tgaccacggg gatgccggtc gcggcgacga aggctgccag atcctcggcg
    14281 gcgtgggcgc ggatgacgcc cccaccgaca tacagcacgg gccgcctcgc ggcctggatc
    14341 agccgcgccg cctcgcgaat ctgtttggcg tgcggcttga tcaccgggtg atagcccgga
    14401 agcgagaccc tgggcggcca gacgaatgtg gtcttggact ggagtgcgtc cttcgcgatg
    14461 tcgacgagca ccggacccgg gcgtcccgtc gcggcgatgt ggaaggcctc ggcgatgacg
    14521 cgcgggatgt cgtcggcgtt cgtcaccagg tagttgtgct tggtgatcgg catcgtgatg
    14581 ccgcgaatgt ctgcctcctg gaaggcgtcc gtgccgatgg cgcgggagga gacctgcccg
    14641 gtgatcgcga cgatcggcac cgagtccatg taggcgtcgg cgatcggcgt gaccaggttg
    14701 gtcgcgcccg gaccggacgt cgccatgcac acgccgacct tgccggtggc ggcggcatac
    14761 ccctctgctg cgtgacctgc gccctgctcg tggcggacga ggatgtgccg gaccgtggtg
    14821 gagtccaaca gcgggtcgta tgccggcagg atcgccccgc ccgggaggcc gaacacggtg
    14881 tcggccccga ccgcttcgag agcgaggatc agcgactgcg cgcccgtgac ctcgactcgg
    14941 ttcgactgcg gctgagcctt gtgggccatc gtcgccggac tcggcgtcgc ggatcgcgcg
    15001 gccactccgt ctgtcgttgt catctcgtat cgtccgctct cagttcggtt ttcgtgtggt
    15061 gtggttgatc cagatcggtc atcgatgcaa taaaaaaccc ctcagtccct gggcgggacg
    15121 tcggggtagc gcgctagccg gacaggggct atgcgcgctc atcgagtacg aggaggaagg
    15181 ccacgggtca actctctccc ccggacgtat gccgcgtcaa cgacgcgccc gtcgcgtctc
    15241 agtggacgga cgcggcgccc aaagcgcggc atctcaaagc gacggagcga tacagacctt
    15301 catgctccgc gggttcatgc tcatgtgccg ggccgtatcg gcatcggcga gcccgtaggt
    15361 gtgagtcacc aactctggtc ttgccctgat aggtgtgctt gtcggtgccg cagatgccgg
    15421 acaggtccat ccgcatcaac gctgcgcccg gctccagccg gggccgggcg aactcctgga
    15481 tcgcgtagcg tcccggctcg agaagcaccg ccgcacgcac tcgctcccac ataccgaggc
    15541 ctcaggggac gattcggacg gcgccgtcgg cggcggaggt ggccatggag gcgtaggccc
    15601 gcagcgccgc ggagacctga cggtcgcggt tgaccggtgt gtaggggcgt tcccgcgcct
    15661 cttgcttggc ccggcgctcg gccaggatct cttcggagac agcgagatcg atcgaccggc
    15721 cggggatgtc gatctcgacc gtgtcgccgt cctcgatgag ggcgatcgcg ccgccgctgg
    15781 cggcctcggg cgagatatgg ccgatcgaca ggccgctcgt gcccccggag aaccggccgt
    15841 cggtgatcaa cgcgcactgc tggcccaggc cgcggccctt gaggaaggag gtgggataga
    15901 gcatctcctg catccccggt ccgcccttgg ggccctcgta gcggatgacg accacgtcac
    15961 cggccgtcac ggacttgtcg aggatcccgg ccaccgcgtc gtcctgggac tcgaagaccc
    16021 gggccgtccc cgtgaatcgc cacaccgact ccggcacgcc ggcggtcttg acgacacagc
    16081 cgtccggggc gatattgccg gtcagcacgg cgaggccgcc gtcgacggtg taggcgtggt
    16141 cgacgctgcg gatgcagccc tccgcctgat cgacatcgac actggcccaa cggttctcgg
    16201 tcgagaaggg ttcggtggtg cggacaccgc ccggagcggc gtggaagagt tcaagggccg
    16261 cctcagtggc ggagccgccg cgaatgtccc actcccccag ccattttgac aaagaaggcg
    16321 agtgcacggc gtgaacgtcc ctctgcagca acccggcccg gtcgagttcg ccgaggattg
    16381 cgggtatgcc gccggcccgg tgcacgtcct ccatgtggta cttcggactg ttgggggcga
    16441 ccttggccag gcatggcacg cggcgcgaga tggcgtcgat gtccgccatc cggaagtcga
    16501 ggtcggcctc gcgggccgca gcgagcaggt gcagcacggt gttggtggag ccacccatcg
    16561 cgacatcgag ggcgaaggca ttggtgaacg catcgtggga ggcgacattg cgcggcagca
    16621 ccgagtcgtc gtcgccgtcg tagtagcggc ggcagagttc gacgatcaag gatccggcgc
    16681 gcaggaagag gtccttgcgc gccgcgtggg tggcgagcgt ggatccgttg cccggcaacg
    16741 acaacccgat cgcctcggtg aggcagttca tcgagttggc ggtgaacatg cccgagca
//
LOCUS       DAOPJG010000057        16779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3631_length_16779_cov_7.829227, whole genome shotgun sequence.
ACCESSION   DAOPJG010000057 DAOPJG010000000
VERSION     DAOPJG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 16779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16779
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3631_length_16779_cov_7.829227"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3421
                     /locus_tag="PKU75_07235"
     CDS             <1..3421
                     /locus_tag="PKU75_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPF80706.1"
                     /translation="LMALETEPNPNLLPAEGIRIRFHSVGGYGTIATGKLLTDILAGV
                     LGMHSKSAPKYGSEKSGAPTNYYITLSPEPILTTNAELEDVEVVIAPDHKAFIHTNPL
                     KGLAEGGTFILQSNLSPEQVWADLPRGARKTIRDKKIRFFVVDAFTVAKKHAPIPELE
                     TRMMGIAFIGAVAAHVDRVAHGASRDQILERVHKQIEKKFGRKGETVVESNMSVILDG
                     IEATTQIDYDTAAFLEIDDHPAPKPLLTVALSASMCPSARAERTSGLFDPTYYEDLMA
                     RPFREGTIGEAPVLPGAGLFMPPGTAAGKDKGLFRREVPAFNPDICTGCMDCALVCPD
                     AAIPNTVHEIHDLILTAVTEIDATDGQKDVLRKEIFGIAERTREALRHREEKPFHEIV
                     ATAAASVATDPTMQHTVDKLVAKLATYPVARTRPFFDSPEKDEPGTGGLFAATIDPWK
                     CTGCLECVEVCGPGALTVQEQDAGVLATLQERFAFMTANPNTPTRFVEGSDSPDGDIK
                     RLMLDHANFWATTGGHGGCRGCGEVTAIRLVMSTSHALGERRRKAHTKELIGLIDRLY
                     AKASTVDSDERRERINGAIKTLEHRLYLLEGGPTGTGPASTIIANATGCSSVYASTMP
                     FNPYIDPWVNSLFQDAQPLAKGIFEGTAAQLVTDVKAMRLAELELSDSYDPAVHDKQL
                     AMLGWGDFTKPELDLMPTVLTIGGDGASYDIGFGAMSRILASDTPIKVMVLNSGVYSN
                     TGGQASTASLTAQDSDLSRFGSAHDGKHESRKELGLLASFHPHVFVCATSTAIQGHFL
                     TNAMEFLRYTDAPAVLDIYTPCGSEHGISEDASAARARLAVESRMNPVFVHDPRRGST
                     LHDWFSLEGNPDIEKTWTTTDLAYKDDDGNIQLMKAALTPAEFALGETRFRKQFHRLP
                     TDLEPVAVPMDEYIELPKEQQASKVPFIWATDDDQKLIKVQCSASIVSLVKDRRRYWQ
                     MLQYLSGVHEAQLTALHTSDLKDLQARYDEAMRARENAIDEIANAMSSLASSSRAPVS
                     LGSAIPVRPSGAGGMPGGAAPAAAPAEAATAVIDAPIWLDEVDIPKCNDCGTCYQELP
                     QLFEQHTMIIDGLPQKVARMIPGALESVEITPDLEKRMDRVKKTCDAEIIQ"
     gene            3418..6240
                     /locus_tag="PKU75_07240"
     CDS             3418..6240
                     /locus_tag="PKU75_07240"
                     /inference="COORDINATES: protein motif:HMM:NF009415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPF80707.1"
                     /translation="MTGELHEWLAASDQLAATQAQVATLEHSDAVESYNRQIELLKRR
                     LIAEPATFRQMFIDDGMNAVMWEFSQDELGADFVKELWKVLLREDDASRVIMRFLWAQ
                     PLKGKRKFVRALDAHLSDRYPMFKGLSVDWPAGNSIPPHIREPEDRSHDFGLVNEGYL
                     GYMNVGYSRAEVDLFVWLEALRDKQCEEKPCEVGVHIAGKPDLKGGCPVKIHIPKVLE
                     LVGTGKFHEAMELIEACNPLPDVTGRVCPQELQCQGVCIQNKMPIAIGQLEWFLPERE
                     KLVNPDGDAARFAHRRDPWLVASKPPVAIIGSGPSGLINAYLLAAEGFPVTVFEAFHA
                     LGGVLRYGIPEFRLPNELVDDVVGKIKLLGGKFVSNFVVGKTATIEQLRENGFYKVFV
                     GTGAGLPRFMDVPGEHYLNVMSANEFLTRVNLMQGLRDDYETPLPEVKDKEVLIIGGG
                     NTAMDAARTSKRLGGNVTIVYRRTQSEMPARVEELEHALEEGIKLLVLRGPTEFLGDD
                     KTGFVSAAILDVMELGEPDASGRRRPVPTGQTETMKADLVIMALGNAANPIVKDSEPT
                     LHTTKWGTIDLEHKGSQETSIEGVYSGGDAARGGSTAINAAGDGQAAAREIIGHLDVP
                     ADQIPLMVAKAKDYTERASASQVIVAKAHLADGIEEIIVHSPVIAEAAQAGQFVRVLP
                     WEDGELIPLTLADWDTEKGTICLVIQGVGTSSIEMNQMNVGDSFAGIAGPLGRPSEIN
                     AYDPATETVVFTAGGLGLPPVYPIAREHLRRGNHVTLIAGFRSASLLFWTGEDERMAG
                     LQREFPDTLEVVYATNDGTFGVEGFVTTPLETMLEAARAGTGRRIAEVVTIGPPLMMR
                     AVADLAKKYDVHTVASLNSIMVDATGMCGACMVPVTIEGKTVRKHACIDGPEIDAHII
                     DWDKFLPRFGQFRKQEQASRARHGLA"
     gene            complement(6390..7190)
                     /locus_tag="PKU75_07245"
     CDS             complement(6390..7190)
                     /locus_tag="PKU75_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPF80708.1"
                     /translation="MSRDVSAHLTARVTQEAEIVLSLATAYRPSEEELSITVDGAPVP
                     FEQVVSTRDTRLHIVPKTPVGTLEVRYAAKLFGDLPIDPRHDGPADRILYLRPSRYCE
                     SDTLAEVAAAEFEGLAGKDLLDGVSSWVGQRLAYVSGSSRPTDGAVQTYLSRAGVCRD
                     FAHLVIALLRARGVPARLVSVYAPGLSPMDFHAVAEACLDDRWYVVDATTLAPRAGMV
                     RIATGRDASDTAFLTVHSGRLDLTGIEVNATMSPALPDDDVTALVSLA"
     gene            complement(7222..8130)
                     /locus_tag="PKU75_07250"
     CDS             complement(7222..8130)
                     /locus_tag="PKU75_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPF80709.1"
                     /translation="MKAVRAEHHSSRRYEVRHVTTYAYPDDVTSSYGRACLRPRDTPE
                     QQVLEHTMQIDPVPDAFVEHVDLFGNYSHYLEIHTPHRTLRVAKQSILWVHRPAPDLG
                     ALDAWTVAAAAAAPDREAPLERAAYLLPSTLVDLAPEVRAYAERYLPPDAGLGAGLAA
                     LTTGIFTDFRYAKGATTVRTTLPQLLQIGAGVCQDFAHLAIGALRSVGIPARYVSGYL
                     ETSPPPGKPKLQGSDATHAWVSVRTPDGSWLDLDPTNDCVADSRYLITAWGRDYRDVS
                     PLKGVIFTEGSGSSLDVSVDVTRLPD"
     gene            complement(8127..10553)
                     /locus_tag="PKU75_07255"
     CDS             complement(8127..10553)
                     /locus_tag="PKU75_07255"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HPF80710.1"
                     /translation="MPSQLLAALAALTPQDLLRRRGAVAQLVQDDGIAVGPDGPDGPH
                     PLRLDPLPLVIPAADWALLEAALAQRAELLDLLLADIYGERRLIDGGVLPAEIIIGHP
                     QFVAQADGISTRHRLVLTAADLGRDEAGEWRLLADRTQAPVGAGYAMATRRVTARALA
                     AVHRETDLRRLREFFDAMRIALQESASETSDLAHVAILTDPEGGETGFDEALTATLLG
                     FPIVHAEDLSLDGGRAWLRTTDRPEPIDVIVRRVNGGRADPLDLTGGPGGGVPGLVEA
                     TRRGVVTVVNPIGAGVLENPALAAYLPAISRALLDQDPLLPAARTWWCGDAESLRYVG
                     AHLEDLQLAPISPAPDNPRVFVAGLTAAERAELWTRVQAQPWAWAASEVLPPWMNDVV
                     TPDGLSPRASVLRTFTVAGSGSPVVLPGGLARLATEAGDRRVPGLRDAIAKDVWVVGE
                     GPGPRASGATPAEPMITGSHGRVASVPLPPRAADDLYWFGRYAERAESTARLIMVADD
                     LVEDHVRRPGTPGYEAMQVLLDAIDGVTVVHRDRPSRRDAAPRQQADRPSAPLAHLHT
                     LTFDGARSGTVRYSARRAARAAGQVRQVLSLDTWIVLSRLERTLTTPIDPSEQLQSVL
                     ARTIESFLALSGIAAEGMIRDASWAFFDAGRRVERAQETVRLLRHALGEPHARRVDTL
                     VLDAVLRSRESLISHRRRVAERHPADTRLAIALDLLLTDASNPRALTYQLTQLVADVG
                     TVKTPVREALSAATTVLDGGDIVALARDREQLTAWLRELESSVRAVSKALDTTTLRHR
                     SPGLAFEEPR"
     gene            10703..12229
                     /locus_tag="PKU75_07260"
     CDS             10703..12229
                     /locus_tag="PKU75_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HPF80711.1"
                     /translation="MFTADRQVRPAYEQVVGGLERIDPTEMRTRVEFLQHIYADQGVT
                     FDIGGQETPFPLDLLPRIIQPADWAVVEAGVRQRVAAQERFLADVYGEGDLFRDGVIP
                     RQVVTSSHHFHRAAAGLRPVNDVRIHVAGIDLIRDPEGRFRVLEDNFRVPSGVSYVMT
                     NRRAITSALPEVAGEHRIRPVQTYPSRLRAALTACAPRGIDDPTIVVLTPGVYNSAYF
                     EHALLARMMGVELVEGRDLLCQNGHVSVRTTRGLSPVHVIYRRVDDEYLDPSQFRADS
                     LLGVAGLINAARRGNVTIANAVGNGVADDKLVYTYVGDLIRYYLGQEPVLPGVDTWRL
                     EEPAHREEVLDRLDELVVKPVDGSGGKGISIGPASSRAELDALRQRIIDDPRGWIAQP
                     VVQLSTVPTVIDGTLAPRHVDLRPFAINDGSDVWVLPGGLTRVALPEGELIVNSSQGG
                     GSKDTWVLEDDSAPEPVVIPNAFVPESISLSVQPPDHNLVEPHSKQQEQQQQGGAGGC
                     "
     gene            12223..13152
                     /locus_tag="PKU75_07265"
     CDS             12223..13152
                     /locus_tag="PKU75_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HPF80712.1"
                     /translation="MLSRIAESLYWIGRYVERAEDTCRILDVHLQLMVDDPSTDPQES
                     ATTLLAVLGMPGTTGPVAPEELLTMLQYDHTSTCSVLASLEGAREAARRARETVSPEM
                     WESLNATWNAVRAGQLRRMRPVAGLTYVRERCALIAGMADSTMSHDEGWHFLRLGRAI
                     ERVDMSARLMLWAAVSRRSSSAWNNALRACGAIHAFRRTHGAATLDEDAAEFLLVDRL
                     FPRSVVYSLTMAESALAELEPGSLRSGFDSEALRILGRARTELEYEPLSELLTELPEA
                     LARLQQVCISVDAAISQRYFVGALSPAWTGGGV"
     gene            13149..13991
                     /locus_tag="PKU75_07270"
     CDS             13149..13991
                     /locus_tag="PKU75_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPF80713.1"
                     /translation="MSTTLRVTHRTGYHYAGAATSSFNQARMTPVSNVDQSVLSTRME
                     VSPPAWTHAFVDYWGTWVTAFEVHRHHEELQVLSTSTVDVHRRQDLYRSASWETLRSP
                     KVLDDFAEYLEEGPLVAAAPELADAVRALAESSAAPNEFVTYVRDLVGQRISYLPGVT
                     SVTTTAEEVWTKGAGVCQDLSHLLIGALRSAGVPARYVSGYLMAGDGAVGEPTTGESH
                     AWLEFWAGDWVGLDPTNSGEVANHHIVVGHGRDYSDVAPLRGIFTGGPAVDMFVEVSV
                     TRLR"
     gene            complement(14007..15269)
                     /locus_tag="PKU75_07275"
     CDS             complement(14007..15269)
                     /locus_tag="PKU75_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPF80714.1"
                     /translation="MSNPVQGALARRLGTGDAVIVGLSAMIGAGIFAAFPPAARAAGS
                     WLLPALGTAALVALCNAIASAQLAQSYPSSGGTYLFGREVLGPWWGYAAGWGFVVGKT
                     ASCAAMAMTFAAYAVPAGAWGRRLVAVVAVVVLTGLNLRGVTRTAYAARILLGITGIV
                     LAGLLVLLWSQSGAAPPGQLVAPAGGVFGVLQGAGLLFFAFAGYARIATLAEEVRRPQ
                     LLTRAILIALGITMALYLAIGTGLVRALGAALPGTDTPLALAVEEVGAGWYAPVVRVG
                     AAAASLGALLALLAGVTRTGLAMARERDLPTWLASVDPRFQVPDHAQVLLGVVVSALV
                     LVADLRGAIGFSSFGVLIYYAVANLAALRQPIEQRRSPRLLQLIGLIGCLALVGTLPP
                     ASVLAGLAVFAVGIVGRLLLRSENGGGH"
     gene            complement(15280..15429)
                     /locus_tag="PKU75_07280"
     CDS             complement(15280..15429)
                     /locus_tag="PKU75_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80715.1"
                     /translation="MNENTCWFCSDDRLGEGVPGADATAAALRLALAPLAQRLRVHNG
                     VIRIP"
     gene            15779..16156
                     /locus_tag="PKU75_07285"
     CDS             15779..16156
                     /locus_tag="PKU75_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPF80716.1"
                     /translation="MLDTPPGLSEFPHVIVNQARMRQFLLDYMARQASRLVPEYAVEF
                     LSLTVDESHGARAVEATLRRGDGEEVTVRASYVVGCDGARSAVWPSAARWSVTPPRLG
                     CDGRPRQHRLPRLAHQERRPVGR"
     gene            16089..>16779
                     /locus_tag="PKU75_07290"
     CDS             16089..>16779
                     /locus_tag="PKU75_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPF80717.1"
                     /translation="MDVLANTDFPDWRTKNVVQSAGKGSMLMIPREDGTMVRCYVDLG
                     EVPLGEKTIRSTTVEQMTARANAILRPYSIDVRSVAWSSVYEVGQRLTDSFDDVPIES
                     AGERLPRVFIAGDACHTHSAKAGQGMNVSMQDDFNLGWKLVTVLSGRADPTLLQTYSA
                     ERQAVAQDLIDFDRFWSGFIAQPMADPANPQAGGVTADQMRAEFERQGRYTAGLATRY
                     EASRLTGSGENQ"
BASE COUNT         2768 a         5662 c         5890 g         2459 t
ORIGIN      
        1 actcatggcg ctcgagaccg agccgaaccc gaatctcctt ccggcagaag ggatacggat
       61 tcgcttccac tccgtcggcg gttacggcac gatcgcgacg ggcaagctgc tcaccgacat
      121 cctcgccggc gtcctcggga tgcacagcaa gtcggcgccg aaatacggct cggagaagtc
      181 cggcgcgccg acgaactact acatcacgct ctcgccggag ccgatcctga cgaccaacgc
      241 cgagttggag gacgtcgaag tcgtcatcgc gcccgaccac aaggcgttca tccacaccaa
      301 cccgctcaag ggcctggccg agggtggcac gttcatcctc cagtccaacc tcagccccga
      361 gcaggtctgg gcggacctgc cgcggggtgc gcgaaagacg atccgtgaca agaagatccg
      421 cttcttcgtc gtcgacgcct tcacggtggc caagaagcac gcgccgatcc ccgaactcga
      481 gacccgcatg atgggcatcg cgttcatcgg tgcggtcgcg gcccacgtcg accgcgtcgc
      541 ccacggtgcc tcgcgcgacc agatcctcga gcgcgtccac aagcagatcg agaagaagtt
      601 cggccgcaag ggcgagaccg tcgtcgagag caatatgtcc gtcatcctcg acggcatcga
      661 ggcaacgacg cagatcgact acgacaccgc ggccttcctc gagatcgacg accacccggc
      721 gccgaaaccc ctgctcacgg tggcgttgtc ggcgtcgatg tgcccgagcg ctcgcgcgga
      781 gcggacttcc ggcctgttcg accccaccta ttacgaggac ctgatggcgc ggcccttccg
      841 cgagggcacc atcggcgagg ccccggtgct cccgggtgcc ggtctgttca tgccgcccgg
      901 caccgccgcc ggcaaggaca agggcctctt ccggcgcgag gtgccggcgt tcaaccccga
      961 catctgcacc ggctgcatgg actgcgcgct ggtctgcccg gacgcggcga tccccaacac
     1021 ggtccacgag atacacgacc tgatcctcac ggcggtcacc gagatcgacg ccaccgacgg
     1081 acagaaggat gtgctccgca aggagatctt cggcatcgcc gaacgcaccc gagaagcgtt
     1141 gcggcacagg gaagaaaagc cgttccacga gatcgtggcc accgccgcgg cgagcgtcgc
     1201 gacggacccg accatgcagc atacggtcga caagctcgtg gccaagctcg cgacctaccc
     1261 ggtggcgcgc actcgcccgt tcttcgactc acccgagaag gacgagccgg gcaccggcgg
     1321 cctgttcgcg gcgacgatcg acccgtggaa gtgcaccggc tgcctcgagt gcgtcgaggt
     1381 ctgcggaccg ggggccctga ccgtgcagga gcaggacgcc ggcgtgctcg ccacgctcca
     1441 ggagcgcttc gcgttcatga cggcgaaccc gaacacgccc acccgcttcg tcgagggctc
     1501 cgacagcccc gacggcgaca tcaagcggct catgctcgac cacgccaact tctgggcgac
     1561 caccggcggg cacggcggct gccgcggctg cggtgaggtg acagccatcc ggctcgtcat
     1621 gtcgacctcg cacgccctcg gcgagcggcg gcgcaaggcg cacaccaagg agctcatcgg
     1681 cctgatcgac cggctctacg ccaaggcctc gaccgtcgac agcgacgagc gacgcgagcg
     1741 gatcaacggc gcgatcaaga cgctcgagca ccggctctat ctgctcgagg gcggcccgac
     1801 cggcaccggc ccggcgtcga cgatcatcgc caacgccacc ggctgcagca gcgtctacgc
     1861 ctcgacgatg cccttcaacc cctacatcga cccgtgggtc aacagcctct tccaagacgc
     1921 ccagccactg gccaagggca tcttcgaagg cactgcggcg caactggtta cggatgtcaa
     1981 ggcgatgcgc ctggccgagc tggagctgag cgacagctat gacccggccg tccatgacaa
     2041 gcagttggcg atgctcgggt ggggcgactt caccaagccc gaactcgacc tgatgccgac
     2101 cgtgctgacc atcggtggcg acggcgcctc ctacgacatc ggcttcggcg cgatgtcgcg
     2161 gatcctcgcc tccgacaccc cgatcaaggt catggtcctc aactccggcg tctattccaa
     2221 caccggcggg caggcctcca cggcgagcct gacggcgcag gactccgacc tctcccgctt
     2281 cgggtcggcg cacgacggca agcacgagtc ccgcaaggaa ctcggcctgc tcgcctcctt
     2341 ccacccgcac gtcttcgtgt gcgcgacgag caccgccatc cagggacact tcctgaccaa
     2401 tgcgatggag ttcctccgct acaccgacgc gccggcggtg ctcgacatct acaccccgtg
     2461 tggatccgag cacggcatca gcgaggacgc ctcggcggcc cgcgctcggc tcgcggtcga
     2521 gtcccggatg aacccggtct tcgtgcacga cccacggcgc ggctcgaccc tgcacgactg
     2581 gttctccctg gagggcaacc ccgacatcga gaagacctgg acgacaacgg atctcgccta
     2641 caaggacgac gacggcaaca tccagctgat gaaggccgca ttgacgccgg cggagttcgc
     2701 gctcggcgag acccgcttcc gcaagcagtt ccaccggctg ccgaccgacc tcgaacccgt
     2761 agcggtgccg atggacgaat acatcgaact gcccaaggag cagcaggcct ccaaggtgcc
     2821 cttcatctgg gccaccgacg acgaccagaa gctcatcaag gtgcagtgct cggcctcgat
     2881 cgtctcgctc gtgaaggacc ggcgccgcta ctggcagatg ctccagtacc tctccggcgt
     2941 ccacgaggcc cagctgacgg cactgcacac ctccgacctg aaggacctgc aggcgcggta
     3001 tgacgaggcg atgcgggcgc gcgagaacgc gatcgacgag atcgccaacg cgatgagttc
     3061 gctcgcctcc tccagccgcg ctccggtctc gctcggctcg gccatcccgg tgcgcccgag
     3121 cggcgccgga ggtatgccgg gtggcgcagc cccggcggcc gcgccggccg aggctgcaac
     3181 cgccgtgatc gacgcgccga tctggctgga cgaggtcgac atcccgaagt gcaacgactg
     3241 cggcacctgc tatcaggagc tgccgcagct gttcgagcag cacacgatga tcatcgacgg
     3301 gctgccgcag aaggtcgccc ggatgatccc cggtgcgctc gaaagcgtcg agatcacgcc
     3361 ggatctggag aagcggatgg accgggtcaa gaagacctgc gacgcggaga tcatccaatg
     3421 accggcgaac tgcacgagtg gctggccgcc agcgatcaac tcgccgccac ccaggcgcag
     3481 gtcgccacgt tggagcacag cgacgcggtg gagtcctaca accggcagat cgagttgctc
     3541 aagcggcgcc tgatcgccga gccggcgacc ttccggcaga tgttcatcga cgacggcatg
     3601 aacgccgtga tgtgggagtt ctcgcaggac gagttgggcg ccgacttcgt caaagaattg
     3661 tggaaggtgc tgctgcgcga ggacgacgcg tcccgggtca tcatgcgctt cctgtgggcg
     3721 cagccgctga agggcaagcg caagttcgtc cgggcgctcg atgcccatct ctccgaccgc
     3781 tatccgatgt tcaagggcct gtcggtggac tggccggcgg gcaactccat cccgccgcac
     3841 attcgcgaac ccgaggaccg ctcccacgac ttcggtctgg tcaacgaggg ctatctcggc
     3901 tatatgaacg tcggctacag ccgggccgag gtcgacctct tcgtgtggct ggaagcgttg
     3961 cgcgacaagc aatgcgagga gaagccgtgt gaggtcggcg tgcacattgc cggcaagccg
     4021 gacctcaagg gcggctgccc ggtcaagatc cacatcccca aggtgttgga gctcgtcggg
     4081 accggcaagt tccacgaggc gatggaactg atcgaggcgt gcaacccgct gcccgatgtc
     4141 accggccgcg tgtgcccgca ggagttgcag tgccagggcg tgtgcatcca gaacaagatg
     4201 ccgatcgcca tcggccaact cgaatggttc ctgcccgagc gggagaagct cgtcaacccc
     4261 gacggcgatg cggcacgctt cgcccatcgg cgcgacccgt ggctggtggc cagcaagcct
     4321 ccggtggcca tcatcggctc ggggccgtcg gggctgatca acgcctatct gcttgccgca
     4381 gagggcttcc cggtgacggt gttcgaggcc ttccatgcgc tcgggggcgt gctgcgctac
     4441 ggcatacccg agttccgcct ccccaacgag ctcgtcgacg acgtcgtcgg caagatcaag
     4501 ctgctcggcg gcaagttcgt ctccaacttc gtcgtcggca agacggcgac gatcgaacag
     4561 ttgcgggaga acggcttcta caaggtcttc gtcggcaccg gcgccgggct gccccggttc
     4621 atggacgtgc ccggcgagca ctacctgaat gtcatgtccg ccaacgagtt cctcacccgc
     4681 gtcaacctca tgcaggggct gcgcgacgac tacgagacac cgctgcccga agtcaaggac
     4741 aaggaagtcc tcatcatcgg cggcggcaac acggccatgg atgcggctcg cacctccaag
     4801 cgcctgggtg ggaacgtgac gatcgtctat cgccgcaccc agtccgagat gccggcccgc
     4861 gtcgaggagt tggagcacgc gctggaggag ggcatcaagc tgctcgtcct gcgtggtccg
     4921 acagaattcc tcggcgacga caagaccggc ttcgtctccg ccgccatcct cgacgtcatg
     4981 gaactcggcg agcccgacgc gtccggccgc cggcgcccgg tccccaccgg ccagaccgag
     5041 acgatgaagg ccgacctggt catcatggcg ctcggcaacg ccgccaaccc gatcgtcaag
     5101 gactccgagc cgacactgca taccaccaag tggggcacga tcgacctgga gcacaaggga
     5161 tctcaagaaa cgtccatcga gggggtgtac tccggaggag acgccgcccg cggcggatca
     5221 acggcgatca acgccgcggg cgacggccaa gccgccgcgc gcgagatcat cgggcatctc
     5281 gacgtccccg ccgaccagat cccgctcatg gtggccaagg ccaaggacta caccgagcgc
     5341 gcctcggcga gccaggtcat cgtcgccaag gcgcacctgg ccgacgggat cgaggagatc
     5401 atcgtgcact cgccggtgat cgccgaggcg gcgcaggccg gtcagttcgt gcgcgtgctg
     5461 ccgtgggagg acggggaact catcccgctc accctcgcgg actgggacac cgagaagggc
     5521 acgatctgcc tggtcatcca gggcgtcggc acctcgagca tcgagatgaa ccagatgaac
     5581 gtcggtgact ccttcgccgg gatcgccggc ccgctgggcc ggccgagcga gatcaacgcc
     5641 tacgacccgg ccaccgagac cgtcgtcttc accgccggcg ggctcggcct gccgccggtc
     5701 tatccgatcg cccgcgagca cctgcggcgc ggcaaccacg tcacgctgat cgccggattc
     5761 cgttctgcct cactgctgtt ctggacgggc gaggacgagc ggatggctgg gttgcagcgg
     5821 gagttcccgg acacgctgga ggtcgtctac gcgaccaacg acggcacctt cggcgtcgag
     5881 ggtttcgtca ccacgccgct ggagaccatg ctcgaggctg ctcgagcggg gaccggtcgg
     5941 cgcatcgccg aggtcgtcac catcggcccg ccgctgatga tgcgggccgt cgccgatctc
     6001 gccaagaagt atgacgtgca caccgtcgcc agcctcaact ccatcatggt cgacgccacc
     6061 ggcatgtgcg gcgcctgcat ggtcccggtg accatcgagg gcaagaccgt gcgcaagcac
     6121 gcctgcatcg acggccccga gatcgacgcc cacatcatcg actgggacaa gttcctgccc
     6181 cgcttcggac aattccgcaa gcaggagcag gccagccggg cccgccacgg cctggcttga
     6241 gggtcgatcg ctgccggacg ccactcgcgg tctacgcccg gcagcggtcg atgccctgga
     6301 cccgaaagac agtggagagt gcacgacgcg ccgtaaacac cgagtgttaa cggcgtgtcg
     6361 tgcgctctcg acgggggtcg cgtctcgtct caggccagcg agaccagcgc ggtgacgtcg
     6421 tcgtcgggga gggcggggga catcgtcgcg ttgacctcga tcccggtcag gtcgaggcgc
     6481 ccggagtgca cggtgaggaa cgcggtgtcg gaggcgtcgc ggccggtcgc gatccggacc
     6541 atcccggcgc gcggggcgag ggtggtggcg tcgacgacat accaccgatc gtccagacag
     6601 gcttcggcca cggcgtggaa gtccatcggc gacagacccg gggcatagac cgacaccagc
     6661 cgcgccggca cgccccgcgc ccgcagcagc gcgatgacca ggtgggcgaa gtcgcggcat
     6721 actccggccc gggacagata ggtctgaacg gcgccgtcgg tcgggcggct cgagccgctg
     6781 acataggcga gccgctggcc cacccaggag ctgaccccgt cgagcaggtc cttgccggcc
     6841 aacccctcga actcggccgc cgcgacctcg gcgagggtgt cggattcgca gtagcggctc
     6901 ggccgcagat agagaattcg atcagccgga ccgtcgtgtc ggggatcgat cggcaagtcg
     6961 ccgaaaagct ttgccgcata acgcacttcg agcgtgccaa caggcgtctt gggcacgatg
     7021 tgcaggcgcg tgtcgcgggt cgagaccacc tgttcgaaag gcacgggcgc accgtcgacg
     7081 gtgatgctca gctcttcctc cgaggggcgg tatgccgtgg cgaggctcag cacgatctcc
     7141 gcctcctgcg tcacgcgggc ggtgaggtgg gcggagacgt cgcggctcag cgcgggattg
     7201 tcggtcatgg cgggggtcct gtcagtcggg caatcgagtg acgtccacgc tgacgtcgag
     7261 ggaactgccg cttccctcgg tgaagatcac gcccttgagt ggggagacgt cgcggtagtc
     7321 gcggccccag gcggtgatca ggtagcgcga gtctgccaca cagtcgttgg tcggatcgag
     7381 atcgagccag ctgccgtccg gcgtgcgcac cgaaacccac gcgtgggtcg cgtccgaacc
     7441 ctgaagtttg ggtttgcccg gcggcgggct cgtctcgaga tagccgctaa catagcgtgc
     7501 cgggatgccc acggagcgca gggcgccgat ggccaagtgg gcgaagtcct gacagacacc
     7561 ggcgccgatc tgcagcagtt gcggcagggt ggtgcgcacc gtcgtcgcgc ccttggcata
     7621 gcggaagtcg gtgaagatgc ccgtcgtcag agcggccagc ccggcgccca ggcccgcgtc
     7681 cggggggagg tagcgctcgg catacgcccg gacctcgggg gcaaggtcga ccaaggtgga
     7741 gggcagcagg tatgccgcgc gctccagcgg cgcctccctg tccggggccg cggcggcggc
     7801 cgccacggtc cacgcgtcca gggcaccgag gtcgggcgcg ggccggtgga cccagagaat
     7861 gctctgcttg gccacgcgca gggtgcggtg cggggtgtgg atctcgagat agtggctgta
     7921 gttgccgaag aggtcgacat gctcgacgaa ggcatcgggc accgggtcga tctgcatggt
     7981 gtgctcgagc acctgctgct cgggggtgtc tcgcggacgc aggcaggccc ggccgtagga
     8041 cgaggtgaca tcgtcgggat aggcatacgt cgtcacatgg cgcacctcat agcgccgcga
     8101 cgaatgatgt tcggcgcgaa cggctttcac cgcggctcct cgaacgccaa accgggcgag
     8161 cggtgccgca acgtggtcgt gtcgagagcc ttggacacgg cgcgcacgga cgattccagt
     8221 tcgcgcagcc acgccgtcag ctgctcgcgg tcccgggcca aggcgacgat gtcgccgccg
     8281 tcgagcaccg tggtcgcggc gctcagggct tcgcgcacgg gcgtcttcac cgtgccgaca
     8341 tccgcgacca gctgggtgag ttgataggtc agcgcgcggg ggttgctcgc gtcggtcagg
     8401 agcaggtcca aggcgatcgc gaggcgcgtg tcggcggggt gccgctcggc gacccggcgg
     8461 cggtgggaga tcaggctctc ccgagaccgc aggaccgcat cgagcacgag cgtgtcgaca
     8521 cggcgggcat gcggttcgcc gagggcgtgc cgcagcagcc gcacggtttc ctgggcgcgc
     8581 tcgacccgcc ggccggcgtc gaagaaggcc cagctggcat cgcggatcat gccttcggcg
     8641 gcgattccgg agagcgcgag gaaggactcg atggtgcggg cgagcacgga ttggagttgt
     8701 tcgctggggt cgatcggcgt cgtgagggtg cgttcgagcc ggctcaggac gatccacgtg
     8761 tcgagcgaaa ggacttgtcg cacttgacca gcggcgcgtg ctgcccggcg tgccgaatag
     8821 cgcaccgtcc ccgagcgggc gccgtcgaag gtgagcgtat gcaggtgcgc gagcggtgcg
     8881 ctcggccggt ccgcctgctg ccggggagcg gcgtcccgcc gggagggtcg gtcgcggtgc
     8941 acgacggtga cgccgtcgat ggcgtcgaga agcacctgca tcgcctcgta tccgggtgtg
     9001 ccggggcgcc gcacgtggtc ctcgacgagg tcgtcggcca ccatgatcag ccgggcggtg
     9061 gactccgcgc gctcggcata ccggccgaac caatacaggt cgtcggccgc gcgtggcggc
     9121 agcgggaccg acgcgacgcg cccatgactg ccggtgatca tcggctcggc gggtgtcgcc
     9181 ccggacgcgc gcggccccgg gccttcgccg acgacccata cgtccttggc gatggcgtcg
     9241 cgtagtcccg gcacgcggcg gtcaccggcc tccgtggcca gccgcgccag accgcccggc
     9301 agcaccaccg ggctgcccga gccggcgacg gtgaaggtgc gcagcacact cgcgcggggg
     9361 ctcagcccgt ccggcgtgac gacgtcgttc atccacggcg gcagcacttc cgatgccgcc
     9421 caggcccacg gttgcgcctg gacccgcgtc cacagttcgg cccgctccgc ggcggtcagg
     9481 ccggcgacga agacccgcgg gttgtcgggc gcgggagaga tcggggcgag ttgcaggtcc
     9541 tcgagatggg caccgacata ccgcaaggac tcggcgtcgc cgcaccacca ggtgcgggcg
     9601 gcgggcagca gcgggtcctg atcgaggagg gcgcggctga tcgcgggcag gtatgccgcg
     9661 agggccgggt tctccagcac cccggcgccg atcgggttca cgaccgtgac gacgccgcgt
     9721 cgggtggcct cgacgagccc ggggaccccg cccccggggc cacctgtcag atccaagggg
     9781 tcggcccggc cgccgttgac gcgccgcacg atgacatcga tgggttcggg ccgatcggtg
     9841 gtgcgcagcc acgcgcgtcc gccgtcgagg gagaggtctt cggcgtgcac gatggggaag
     9901 ccgaggagag tggcggtcag tgcctcatcg aagcccgtct caccgccttc gggatcggtg
     9961 aggatggcca cgtgagccag gtcgctggtc tcgcttgcgc tctcttgcaa cgcgattcgc
    10021 atggcgtcga agaattcccg gagccggcgc agatcggtct cgcggtgcac cgcggccagc
    10081 gcgcgggccg tgacccggcg ggtggccatc gcatagcccg cgccgacggg cgcctgggtc
    10141 cggtcggcga ggaggcgcca ttcgccggcc tcgtcgcggc cgagatccgc cgcggtgagc
    10201 accagccggt ggcgggtcga tatgccgtcg gcctgggcga cgaactgcgg atggccgatg
    10261 atgatctcgg ccggcaggac cccaccgtcg atcagtcgcc gctcgccata gatgtcggcg
    10321 aggagcaggt cgagcagttc ggcacgctgg gcaagcgcgg cttcgaggag agcccagtca
    10381 gccgccggta tgacgagtgg cagcgggtcg agtcgcagcg ggtgcggacc gtcggggccg
    10441 tccgggccga ccgcgatccc gtcgtcctgc accagttgtg cgacggcccc ccgccgccgg
    10501 agcaggtcct gaggggtcag tgcggcgagc gcggccagaa gttgcgaggg catcgcgccc
    10561 agcctatgcg ccgggtgcca cgcgtgggcg ggcggcgggt ggcggtgatc gcgaaacacg
    10621 ccggaaaccc gagggccgta tgttgcggcc tgtgagcgcg tggatcgatg actaccgccc
    10681 gggcgggggt gtctatgacg aaatgttcac ggccgaccga caggtgcggc cggcatacga
    10741 gcaggtcgtg gggggtctgg agcggataga tcccaccgag atgcgcacgc gggtggagtt
    10801 cctgcagcac atctacgccg atcagggggt caccttcgac atcggcggtc aggagacgcc
    10861 gttcccgctg gacctgctcc cgcggatcat ccagccggcc gattgggccg tcgtcgaggc
    10921 gggggtgcgg cagcgggtgg ctgcgcagga gcgcttcctc gccgacgtct atggcgaggg
    10981 tgatctcttt cgtgatggcg tcatcccgcg gcaggtcgtg acgagttcgc accacttcca
    11041 tcgggcggcg gccgggctgc ggccggtcaa cgacgtgcgc atccacgtcg ccggcatcga
    11101 cctgatccgc gacccggagg gccggttccg ggtgctggag gacaacttcc gggtgccgtc
    11161 cggggtctcc tatgtgatga ccaaccggcg ggcgatcacc tcggcgttgc cggaagtggc
    11221 gggggagcat cgcatccgtc cggtgcagac ctatccgagc cggctgcggg ccgcgctgac
    11281 cgcctgcgcc ccgcgcggca tcgacgatcc gacgatcgtc gtgctgacgc cgggggtcta
    11341 caactcggca tacttcgagc acgccctgct cgcccggatg atgggcgtcg aactcgtcga
    11401 aggccgggac ctgttgtgcc agaacggaca cgtgtccgtg cggacaactc gaggcttgag
    11461 ccccgtgcac gtgatctatc gccgggtgga tgacgaatac ttggatccct cgcagttccg
    11521 cgccgattcg ctgctcgggg tcgccgggct gatcaatgcg gctcgccgtg gcaatgtcac
    11581 gatcgccaat gccgtcggca acggggtcgc ggacgacaaa ctggtctata cgtatgtcgg
    11641 ggatctgatc cgctactacc tcggtcagga gccggtcctt cccggcgtcg acacgtggcg
    11701 gctggaggag cccgcgcacc gcgaggaggt gttggaccgg ctcgatgagc tcgtggtcaa
    11761 gccggtcgac ggctccggtg gcaaggggat cagcatcggg ccggcgagtt cgcgcgcgga
    11821 gctggatgcg ttgcggcagc gcatcatcga cgatccccgc gggtggatcg cccagccggt
    11881 ggtgcagctg tcgacggtgc cgacggtcat cgacggcacg ctcgctccgc ggcatgtcga
    11941 cttgcggccg ttcgcgatca acgacgggtc ggacgtgtgg gtgctgccgg gcggcctgac
    12001 ccgggtggcc cttccggaag gcgagctcat cgtcaactcc agccagggcg gcgggtccaa
    12061 ggacacgtgg gtgctggagg atgacagcgc accggaaccg gtcgtcatac ccaacgcctt
    12121 tgtgcccgag tcgatctcgc tgtcggtgca gccgccggat cacaacctgg tcgagccgca
    12181 cagcaagcag caggagcagc aacagcaagg aggcgcggga ggatgctgag ccggatcgcc
    12241 gaatcgctct attggatcgg ccgctatgtg gagcgggccg aggacacctg ccggatcctc
    12301 gatgtgcacc tgcagctgat ggtagacgac ccgtcgaccg atccgcagga atcggcgacg
    12361 accctgctcg ccgtgctggg catgccgggc acgaccggac cggtggcgcc ggaagaactg
    12421 ctcacgatgc tgcaatacga ccacacctcc acgtgctcgg tgctggcgtc cttggagggt
    12481 gcccgggagg cggcccgcag ggcgcgggag acggtgtcgc cggagatgtg ggagagcctg
    12541 aacgcgacgt ggaatgcggt gcgcgccggg caattacggc ggatgcggcc ggtcgcgggg
    12601 ttgacatatg tgcgggagcg gtgcgcgctc atcgcaggca tggccgactc cacgatgagc
    12661 catgacgagg gctggcattt cctgcggctc gggcgagcga tcgagcgggt ggacatgagc
    12721 gcgcggctca tgctgtgggc ggcggtgagc cggcgatcgt cgtcggcgtg gaacaacgcg
    12781 ttgcgcgcgt gcggtgctat ccatgccttc cggcgcaccc atggcgcggc caccctggat
    12841 gaggacgcgg cggaattcct gctcgtcgac cggctcttcc cccgctctgt cgtctattcg
    12901 ctgacgatgg cggagtcggc gctggcggag ctcgaacccg gctctctgcg aagcggtttc
    12961 gacagcgaag cgttgcgcat cctgggccgg gcccgcaccg agctggagta tgaaccgttg
    13021 agcgagttgc tcaccgagct gccggaggct ttggcccggc tgcagcaggt gtgcatcagc
    13081 gtcgacgcgg ccatcagtca gcggtatttc gtcggggcgc tgtccccggc gtggacggga
    13141 ggcggcgtat gagcaccacg ctgcgcgtga cccatcgcac gggctaccac tatgccggcg
    13201 ccgcgacatc gtccttcaac caggcgcgga tgacgccggt gtccaatgtc gaccagtcgg
    13261 tgctctccac gcgcatggag gtctcaccgc cggcgtggac ccatgccttc gtcgactact
    13321 ggggtacatg ggtcacggcc ttcgaggtgc atcgccacca cgaggagttg caggtgcttt
    13381 cgacgagcac cgtcgacgtc caccggcggc aggacctcta ccgctctgcg tcgtgggaga
    13441 cgctgcggtc acccaaggtc ctcgacgact tcgcggagta cctagaggag gggccgctgg
    13501 ttgccgccgc gccggagctg gcggatgcag tgcgcgcact ggccgaatcc agcgctgcac
    13561 caaacgaatt cgtcacgtac gtgcgggatc tggtcggtca gcggatctcc tatctccccg
    13621 gcgtcacgag cgtgaccacg acggctgagg aggtctggac gaagggcgcc ggcgtctgcc
    13681 aggacctgtc gcacctgctc atcggggctc tgcgtagcgc gggggtgccg gcccgatatg
    13741 tgtcgggcta cctgatggcc ggtgacgggg ccgtgggtga gccgaccaca ggggagtcgc
    13801 atgcgtggct cgagttctgg gcgggcgact gggtcggcct cgatccgacc aactcgggtg
    13861 aggtcgccaa ccatcacatc gtcgtcggcc acggtcgcga ctattccgat gtcgcgccgc
    13921 tgcgcggcat cttcacgggc ggcccggccg tggacatgtt cgtcgaggtg tcggtcaccc
    13981 ggctgcggta gcgccgcgcg cgcctgtcaa tggccgccgc cgttctcgga ccgcagcagc
    14041 aggcgtccca cgatcccgac ggcgaagacg gccaggccgg cgagcaccga cgcggggggc
    14101 aacgtgccca ccagcgccag gcagccgatc aatccgatga gctgcagcag gcgcggtgac
    14161 cggcgttgct cgatcggctg ccgcaaggcg gcgagattgg cgacggcgta atagatgagc
    14221 acgccgaagg aggagaaccc gatcgcgcct cgcaggtcgg cgaccagcac gagcgccgag
    14281 accacgacgc ccaagagcac ctgagcgtgg tcgggcacct ggaagcgcgg gtcgaccgac
    14341 gcgagccagg tcggaaggtc gcgctcgcgg gccatcgcca ggccggtgcg ggtgactccg
    14401 gcgagcaggg ccagcagggc gccgaggctg gcggctgcgg ccccgacccg cacgaccggc
    14461 gcgtaccagc cggccccgac ctcctcgacg gccagagcca agggcgtgtc cgtccccggc
    14521 agcgccgcgc cgagggcgcg gacgagccca gtgccgatcg ccagatagag cgccatggtg
    14581 atgccgagcg cgatgaggat ggcccgggtg agcagctgcg gccggcgcac ctcctccgcc
    14641 aaggtggcga tgcgggcata cccggcgaac gcgaagaaca gcagcccggc gccctgcagc
    14701 acgccgaaca cgccgcccgc cggtgcgacg agctggcccg gcggcgccgc cccgctctgg
    14761 gaccagagaa ggaccagcaa gcccgccagc acgatcccgg tgattcccaa caggatccga
    14821 gccgcgtatg ccgtgcgcgt gaccccgcgc aggttgagac ccgtcagcac gaccaccgcg
    14881 accaccgcga ccaggcgacg cccccacgcc ccggcgggca cggcatacgc agcgaaggtc
    14941 atcgccatag cggcgcacga cgcggtcttg ccgacgacga aaccccagcc cgcggcatac
    15001 ccccaccacg gaccgaggac ctcccgcccg aagagatagg tgccgcccga actcggataa
    15061 gactgtgcca gttgagcgct cgcgatggcg ttgcacagcg cgaccagcgc cgcggtgccg
    15121 agcgcgggca gcagccacga gccggcggcg cgtgccgccg gcgggaacgc ggcgaagatt
    15181 ccggcaccga tcatcgcgga cagcccgacg atcactgcgt ccccggtgcc gaggcgccgc
    15241 gccagggcgc cctgcactgg gttgctcatg gtgggcaggc taggggattc ggatgacgcc
    15301 gttgtgaacc cgcagtcgct gcgctaacgg ggccagggcc agccgcaagg ctgccgcggt
    15361 ggcgtcggcg cctggcaccc cctccccgag gcggtcgtcg ctgcagaacc agcaggtgtt
    15421 ctcgttcacg gctcgatgct gacaggccca ggtggtcccg gcttgattca ggttgctgtc
    15481 cgagatggtg actaccggtc gatgcgtgat tggctggtaa cgagatgcga cgcaccgaag
    15541 gagttgtctc ccatgcagtt ctatcgtgac ggctagcggc ccggcgatcc ggatgtgcga
    15601 cagcccgcgc cggaggtagc ggagcgaggc gccggcctgc cgggcgaggt ggatgtgctg
    15661 gtatgttcca cgcgttcggc cttgccgaaa ccctgctgcg agaagcctat tgggtcaacg
    15721 aggtgcgctt ctggggtccc tcggccgcgg accgcgaccg gatcgagcgg acggctgggt
    15781 gctcgacacc ccgcccggac tcagcgagtt cccgcacgtg atcgtcaacc aggcccgcat
    15841 gcggcagttt ctgctcgact acatggcgcg ccaggccagc cgcctggtgc ccgagtatgc
    15901 cgtcgagttc ctctccctga ccgtcgacga gtcgcatggc gcccgggcgg tcgaggcgac
    15961 cctgcgccgc ggcgacggtg aggaggtcac cgtgcgggcg tcgtatgtcg tggggtgcga
    16021 cggcgcgcgc agtgccgtgt ggccatcggc cgcgcgctgg tcggtgacgc caccacgcct
    16081 ggggtgtgat ggacgtcctc gccaacaccg acttccccga ctggcgcacc aagaacgtcg
    16141 tccagtcggc cggtaagggc agcatgctca tgatcccgcg cgaagacggg accatggtgc
    16201 gttgctatgt cgacctcggc gaagtgccgt tgggggagaa gacgattcgc tccaccaccg
    16261 tcgagcagat gaccgctcgc gccaacgcca tcctgcgtcc ctactccata gacgtccgct
    16321 cggtggcgtg gtcgagcgtc tacgaggtcg ggcagcggct gaccgactcc ttcgacgacg
    16381 tcccgatcga gagcgcggga gagcggctgc cgcgagtgtt catcgccggg gatgcctgcc
    16441 acacacactc cgcgaaggcg gggcagggca tgaacgtctc gatgcaggac gatttcaacc
    16501 tcggctggaa gctcgtcacc gtcctgagcg ggcgggcgga tccgactctg ctgcagacct
    16561 attcggccga acggcaggct gtcgcgcagg acctgatcga cttcgaccgg ttctggtcgg
    16621 gcttcatcgc ccagcccatg gccgacccag ccaacccaca ggccgggggc gtgaccgccg
    16681 accagatgcg agccgagttc gaacgccagg gccgctacac ggccgggttg gcgacacgtt
    16741 acgaggcgtc cagactcacg ggatcaggtg agaaccaaa
//
LOCUS       DAOPJG010000058        16740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3641_length_16740_cov_8.426311, whole genome shotgun sequence.
ACCESSION   DAOPJG010000058 DAOPJG010000000
VERSION     DAOPJG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 16740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16740
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3641_length_16740_cov_8.426311"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..746)
                     /locus_tag="PKU75_07295"
     CDS             complement(9..746)
                     /locus_tag="PKU75_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobB"
                     /protein_id="HPF80718.1"
                     /translation="MSEESTKGEVHVVHLYPREMSIYGDLGNTRAMVSRLTRHGYSPV
                     LHQHHPGAPFPEQAHLVLGGGGQDSGQARVEADLEVIAPRLSELAAAGTPMLMICGMY
                     QLFGNAFVTVEGKRLPGLGILDVTTQGNAKRMIGPVVLSTDFGDVVGYENHSGSTRLG
                     SGQAPFGTVRSGFGNNGEDQTEGARTGNVVGTYLHGPILPANPAFADALIGYAAELAT
                     GRPFEPGTLDDAFADQARTRQVRRLVG"
     gene            complement(743..2059)
                     /locus_tag="PKU75_07300"
     CDS             complement(743..2059)
                     /locus_tag="PKU75_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HPF80719.1"
                     /translation="MLRTSAAIAAGKLARAVARRRGGGSAMPGLVAERIDPKILGHAL
                     GGLPDGIVVVSGTNGKTTTTRMLVALLRAHGHSVFTNPTGSNFTRGVISALLGEVGLR
                     GRPVADMAVLELDEAHALKFADAVTPTHALLLNVARDQLDRFAEIDHTAKLLAQLASR
                     TSTGVVINRDDSFVARSADGVKTGVRVDYFGVDASIAHRLPELMEQDTRLDDDYVVPP
                     AGAGDVLLKPRDERAFVILLGADGVGGSVGPVELKQRGLAAMINATAATAMARQLLGG
                     SFDPVRAASALASVTAPFGRGEVIDADGAPLELVLVKNPAGFTVALSTYGSTPVRTMI
                     AINDNYADGRDVSWLYDVSFESLRTIGVALTSGVRAYDMALRLQYDEVAVDAVEPDLA
                     AALDRFLAARDGQPARIFCTYTAMMTLRRILAERYDLPAFGEEATS"
     gene            complement(2074..2835)
                     /locus_tag="PKU75_07305"
     CDS             complement(2074..2835)
                     /locus_tag="PKU75_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPF80720.1"
                     /translation="MTAGHPVGEGNALLDGVMRYVVIGPAFYALARPTVVGREHIPPE
                     GSPAIIASNHLSFSDSMVIPLASGRRVSFLGKAEYFHGTGVKGALMRAWFRSFGTIPV
                     ERTDARAAAESLHLAERVLGAGGAFGIYPEGTRSRDGRLHKGRTGVARLALSTGAPIV
                     PCALIGTDKLQPPDARVLRPTKVTVQFGPAIDVAALQAQFTKAALLRAITDAVMDSIG
                     AMSGQEHSARYASDVNAELDARGSGEAGSREPFAG"
     gene            complement(2832..3794)
                     /locus_tag="PKU75_07310"
     CDS             complement(2832..3794)
                     /locus_tag="PKU75_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPF80721.1"
                     /translation="MTPQSPPRSAFRDAADVLLVFLAGLVLLSVIVVLALRTDAGIRL
                     DRDVVASFEVDEFRPGRISALLQEIRIPTMLLLTLIAMLVVLARRLWRVALALPLLVI
                     GANQTTQLLKLRFLTDMPSDPAVRVSMPSGHSTAAISLAAVAIIAAPRILRPLVCLLS
                     GAIAGGAGLGTMAERWHKPADVIAAVGVVLIWAAVATLIGARWVEPPQVRPAGFDRAV
                     GHSALAVLGVAAGAFVLHRLGMEPVPGARAATLVYASLIVVGLTIGLGLGLIAVLADR
                     RLWRLPRAVAEPVPAVVTGPPTTTTPHTATGPHTTTRPHTEGET"
     gene            complement(3791..5362)
                     /gene="guaA"
                     /locus_tag="PKU75_07315"
     CDS             complement(3791..5362)
                     /gene="guaA"
                     /locus_tag="PKU75_07315"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPF80722.1"
                     /translation="MTTARRPVIVVDFGAQYAQLIARRVREARIYSEVLPHTASVADL
                     LARDPQAIILSGGPSSVYAEGAPALDPALLEAGVPVFGMCYGFQAMAQARGGTVARTG
                     LREYGQTRVEVADTESTLFRGQPREQSVWMSHGDSVTEAPAGMRVTASTAGAPVAAFE
                     DDERRLYGVQWHPEVLHSTFGQEVLEHFLYAGARLTPDWDAGSVIEEQVAQIRAQVGD
                     KHVLCALSGGVDSSVAAALVHKAVGDQLTCVFVDHGLLREGEAEQVEEEFVASTGINL
                     VVVDARQQFLDALAGVRDPEEKRKIIGREFIRVFEQAARDVVGHAAEDGHPVEFLVQG
                     TLYPDVVESGGGSGTANIKSHHNVGGLPDDLQFSLVEPLRDLFKDEVRKVGLELGVPE
                     AIVWRQPFPGPGLGIRIVGEVTADNLATLRAADAIVRHELTRAGLDRDIWQCPVVLLA
                     DVRSVGVQGDGRTYGHPVVLRPVSSEDAMTADWTRVPYDVLAHISNRITNEVEQVNRV
                     TLDVTSKPPGTIEWE"
     gene            complement(5359..5694)
                     /locus_tag="PKU75_07320"
     CDS             complement(5359..5694)
                     /locus_tag="PKU75_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HPF80723.1"
                     /translation="MSTPTTDRDPKLASALKFFEIMAFIVGIGLLVLVAEIILRYGFD
                     NHALDWWPQPHGFLYIVYVAATANLGFKAGWSLPKMVGIMLAGCVPFLSFWVERKVAA
                     EFAATSIPA"
     assembly_gap    5823..5922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5923..6694)
                     /locus_tag="PKU75_07325"
     CDS             complement(<5923..6694)
                     /locus_tag="PKU75_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HPF80724.1"
                     /translation="MLRTAVKPKWLALLLLAALIAIAFVQLGRWQLGVAHDKARADAI
                     AKAGEQPVAELTTLLTPHTEFPAAAGGRLVTATGRYAAGQVLVAGRLLGGVDGYWVIT
                     PLRLTATGATFPVLRGWTPQAVLPEPPGGEVTVTASLAPGESPAEGTYPAGTLGSVNL
                     SELVNTWPGDLYNAFGFAQRETPVSDPQASDAPAPLTRVPPPRPDTGVQWRNAAYALQ
                     WWVFAAFAFFLWVKMVQADARGPAVRGAAGPAPEHAAGP"
     gene            7027..9522
                     /locus_tag="PKU75_07330"
     CDS             7027..9522
                     /locus_tag="PKU75_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF80725.1"
                     /translation="MQGDTTSATSTLRASAAHRQEHEDLAARARTLLNVDPEHLQPVV
                     ITGPIGGLVNSAIRRVAAASDLPGFWLHSLPPRPDGDQRCIVQEAARDGEQDGLAMTA
                     DDMGGLLDQLTAQVQGPALLIVESVQQLSTSSANALVVWAQLARLRGTRLLLGYAPAA
                     DIDAVGTLVAMNPEVHWIDVQSPSPERLRHALERAARWTGADELDRAAAFCAGSPGLT
                     TAVCRAAAIEGEVTDSRFPHVYANALESSAVPQLLRQGPDAVALALVMALAGHTVDAA
                     EAAGALGILPRTARRLERFLRESGLAPDSPEAQVIVHRVVAAQAGQDIRRRAASCARG
                     LLEAHRAQAVDLFALHSLVGDDVAGRMLLAKDIFTAALFEDAFDRALQVAQNLEATSS
                     DPGEIAWARAAQLAVWMRSDWAALHRQREWLTDPAVLAELDGARPSAWLALESPATVE
                     LLLEAPAADGAWSGLDATFLSGRMSTVPTTYLRGVVAAAETGPTYASYLAVCALIGHR
                     ASPRLAALSECAEALGGPLGVGAALASRIAAAWLFLTDYEAAARWAAIAAFTAAENEA
                     ADCAMGYLIGAQAQLRLGNFGRAREQAELAKTNFARFNATTMMDVCVWTLAHIDAEDV
                     AGAEPADAQALAAALPDAVHPLWIAYRAYVCGRTLLRHGETDVAVRYLFGSGRDLARA
                     GIENPSLLNWLPHLVRVFRAGGLTDFHNQVTDDFASRWRRWDRIEPAAAGRRVRALAD
                     VGTELGLVPKESPNEPGPSQLSPAERKVVVVVLEGLSNREVARKLFLSKRTIDTHLGN
                     IYRKLGLSSRDELICWARRFPLSSDGASEAAYG"
     gene            complement(9675..10418)
                     /locus_tag="PKU75_07335"
     CDS             complement(9675..10418)
                     /locus_tag="PKU75_07335"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF80726.1"
                     /translation="MTGPIAWLHGYTMSSRVWEPLWDLLPGRDHLGIDLPGHGSKAKT
                     PMPRSMSGWTDMVAAEMRAAGSTELVGLSFGSSIALQVAADHPDLVRRLVLAAPTLSG
                     VPDDPAARAKYWMLAMRIGELGPGPDIARIWMTDPPRIFTGLREFPQRYAAMADIIAG
                     HPFGELRSGAMATLSSHVQDAAVLGRIRAEVLLIVGTDDMPQFIANTDTIAQAIPAAQ
                     VKIIEGAGHLPLLERPEVSAGLIAGFVGA"
     gene            complement(10415..11392)
                     /locus_tag="PKU75_07340"
     CDS             complement(10415..11392)
                     /locus_tag="PKU75_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HPF80727.1"
                     /translation="MNQTLHAGWYLVATEDELTADITPLDVGGRRLMVTRTASDDGDA
                     IFTVADATCPHRGAHLGYGGVLDRDCVVCPFHGRRIVVGEHPTRPFVSTHPTLQVGPL
                     VFARFATDPVGDCDFSVDFAGLVADREIRVAVHAVVDMPVEFVVENAFDSDHFSAVHQ
                     VPGLGPMTTTALPSGALRISGDFRTMADPWYDMRYAAALNDFVSDYRGTPTRSSGFEA
                     TAYSPTIVSTAFGEGPRPPLIVTMATPTSEGVHIRVAIVGGAHDPLDSIAVGAKIAIA
                     QDIAVWKHIDPDAPWQLDDLDAGVIAFRRFVADFPPAASPAPAPSGASA"
     gene            complement(11389..12609)
                     /locus_tag="PKU75_07345"
     CDS             complement(11389..12609)
                     /locus_tag="PKU75_07345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF80728.1"
                     /translation="MDVVVVGAGVAGLAAAEYAVASGLRVEVLEAADRVGGRVATVTH
                     AGRAAELGAQVVHGSDNPFLSWAGAAPTVRLVDGAQIAAGWLDGETLQPLDCEGDPTG
                     PLRAERLIAGVYRSLGAFMAERLAVTGAVRIAGASVATGDLLRGWTEQIAGIDPGAET
                     LARYLTDAAVRPPPGEKHLLPGGMTTPLEKVAAQLPVTLRTTVRHISIDGAGVVVRSE
                     TGQIRARHVILTAPPSVAHAIDLPDMPAAQRAAGDQLRAIPAAVAILPLGEAAAGDSF
                     AYVPGVGFLTANDGQEHATLVAKGAPAYRLRGWTRDRAAWAKTVTALGVQPDNRREVI
                     VREWHDDPLAGGGFTAPVDPAGDAARQWRTPVGGRLHMAGEAAWADEGHPYVDRAWHS
                     GRRAVDAILEELSR"
     gene            complement(12615..13784)
                     /locus_tag="PKU75_07350"
     CDS             complement(12615..13784)
                     /locus_tag="PKU75_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPF80729.1"
                     /translation="MTTTTDSPAAPPGVAPIFSPLFAINPEPTLSHLREHDPVHRLPG
                     PEPVYVLTRAADAEAALRDPACSAAGGQEERSRRSGAPVSMLNTDGAEHDSLRRPGAA
                     LLGPAAVRASSGALAAAAGNLVSELPARFDITSAYAEPLATLALLEILGLADHARFGD
                     FDRHARAVSVAIDPMPSPAQAAQGALAMGEFDGYVERLVATAPNSLLAQLPSRFGITP
                     EQARGIVSLAVVGGWSPLAEVVTTALELALRDDGPADADLEPWCDDVIRWHTPIPFVA
                     RRTISALTVPSGAIPAGAQVLIHLGSANRDGRRFPDPDRVDAQRPAASAHLAFGHGAH
                     VCLGTALVRAVVPLAIRSLRSQRPGARVASSLFVWRPGIFPRRPMSAIVHGGVAT"
     gene            complement(13784..14650)
                     /locus_tag="PKU75_07355"
     CDS             complement(13784..14650)
                     /locus_tag="PKU75_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF80730.1"
                     /translation="MTMTSSRPTTTASSAPPAPAHRPLPGLWRTSLARSVSELRVFFR
                     NPQSVVFTLALPVILLMIFGSIFSGKIEGTDTDFKQYFVAGIVAAGIMSTAFTGLAIN
                     VALEREMGLIRRLAGSPMPKASYFVGKVVLVLVTSLLEALILIALGTALFGLDLPSDA
                     DHWIAFGWIFVLGTAACALSGLAYTALIPSANSAAAIVTPPFLVLQFISGVFFPFNQL
                     PEWMQTTAGIFPLKWMTQGLRYVFLPDGFSAVEPGGVWNLPLIAMALGFWVLLAGFLT
                     AITFKWRGPKVR"
     gene            complement(14647..15591)
                     /locus_tag="PKU75_07360"
     CDS             complement(14647..15591)
                     /locus_tag="PKU75_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80731.1"
                     /translation="MTTITDHLPIDVQGLRKTYGDFAAVDHLDLQVRPGEVFALLGPN
                     GAGKTTTVDMLCGVQRRTGGTVSVLGEDPHHDSRQWRNRLGVVPQNTGDYLNLTVREV
                     VEMFATFYSDPIPTDELISMVGLTAKAGAKTNALSGGQKRRLDVAVGVVGKPELIFLD
                     EPTTGLDPQARREAWDLVDYFRELGATTVLTTHYLDEAETLADRAGVIAAGRMLAIGT
                     IDELGRTAGYSANVTVTDLEGVAGVIPSDLGVLEPDARADRRGGVLIGTDQPTAVVER
                     LTRLAREQGFGEIPGLTVSRPTLEDTYLRLIQREDAQR"
     gene            complement(15764..16129)
                     /locus_tag="PKU75_07365"
     CDS             complement(15764..16129)
                     /locus_tag="PKU75_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80732.1"
                     /translation="MSSNEFREQYLQLLVKDWTDEGFGARVDADAKGALAEVGIVIPD
                     GVDVEVIRHGQGEVNPYEGKSNEAALDAQEAIFEAGLKSGKVQIHLPAAPSTEEGILS
                     ESDLSEVAAGEVPCCCCCC"
     gene            complement(16188..16574)
                     /locus_tag="PKU75_07370"
     CDS             complement(16188..16574)
                     /locus_tag="PKU75_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80733.1"
                     /translation="MRQAARTQFIKSYSQALLQSWCDEDFAGQLDRNPREALVSVGME
                     LPPGATIEIIRHTPDQVGEARRDGDVDKQVALYEAGLESGHFVFHMPTAPVVDTADLN
                     AEELGVIAAGTTYCCCCCPACSSCTI"
BASE COUNT         2477 a         5736 c         6040 g         2387 t
ORIGIN      
        1 cgcgggggtc agccgacgag ccggcggacc tggcgggtgc gggcctggtc ggcgaaggca
       61 tcgtcgaggg tgccgggctc gaaggggcgg ccggtggcga gttcggcggc atagccgatg
      121 agcgcatcgg cgaaagccgg gttggcgggc aggatcgggc cgtgcaggta ggtgccgacg
      181 acattgccgg tgcgggcgcc ctcggtctgg tcctcgccgt tgttgccgaa cccggaccgc
      241 acggtgccga agggcgcctg cccggatccc aaccgcgtcg agccggaatg gttctcgtag
      301 cccacgacat cgccgaagtc ggtggacagg acgaccggcc cgatcatccg cttggcgttg
      361 ccctgcgtgg tcacgtcgag gatgccgagg ccggggaggc gcttgccttc gacggtgacg
      421 aaggcgttgc cgaagagctg gtacatgccg cagatcatca gcatcggggt gccggcggcg
      481 gcgagttcgc tcagccgggg tgcgatgacc tcgaggtcgg cctcgacccg ggcctgcccg
      541 gagtcctggc cgccgccgcc gaggacgaga tgggcctgct ccggaaaggg cgcacccggg
      601 tgatgctggt gcagcacagg cgaatagccg tgccgcgtca ggcgcgagac catcgcgcgg
      661 gtgttgccca ggtcgccgta gatgctcatc tcccgcggat agagatggac gacgtgcacc
      721 tcgcccttgg tcgactcctc gctcatgacg tggcctcctc cccgaaggcg ggcagatcgt
      781 agcgctcggc caggatgcgg cgcagcgtca tcatcgccgt gtaggtgcag aagatccggg
      841 ccggctggcc gtcgcgggcc gcgaggaagc gatcgagcgc cgccgccagg tcgggctcga
      901 cggcatcgac cgccacctcg tcatactgca gtcgcagcgc catgtcatag gcccgcaccc
      961 ccgacgtcaa cgccaccccg atcgtgcgca acgactcgaa cgagacgtca tagagccacg
     1021 agacgtcgcg gccgtcggcg tagttgtcgt tgatggcgat catcgtgcgc accggcgtcg
     1081 agccataggt cgacagggcg acggtgaacc cggcggggtt cttgacgagc accagttcca
     1141 gcggcgcgcc gtcggcgtcg atcacctcac cgcggccgaa gggcgccgtc acggacgcca
     1201 gcgccgacgc cgcccgcacc ggatcgaacg acccgccgag cagctgtcgc gccatggcgg
     1261 tggccgccgt cgcgttgatc atcgccgcca gcccgcgctg cttgagttcg accggcccga
     1321 cgctgccgcc gaccccgtca gcaccgagga ggatgacgaa ggcgcgctcg tcacgcggct
     1381 tgagcagcac gtcgcccgca cccgccggcg gcacgacata gtcatcgtcg aggcgcgtgt
     1441 cctgctccat cagctcaggc agccggtggg cgatcgacgc gtcgacaccg aaatagtcca
     1501 cgcgcacacc ggttttcacg ccatcggcgc tgcgcgcgac gaaggagtcg tcccggttga
     1561 tcaccacgcc ggtcgaggtg cgcgacgcga gttgcgccag cagcttcgcc gtgtggtcga
     1621 tctccgcgaa ccggtcgagc tggtcgcgag cgacgttcaa caggagcgca tgcgtcgggg
     1681 tgacagcgtc ggcgaacttc agcgcgtgcg cctcgtcgag ctcgagcacg gccatgtcgg
     1741 ccaccggtct gccgcgcagc ccgacctcgc ccagcagcgc cgagatcacg ccgcgcgtga
     1801 agttgctgcc cgtgggattg gtgaagacgc tgtgcccgtg ggcgcgcagg agggcgacga
     1861 gcatgcgcgt ggtcgtcgtc ttgccgttgg tgccggagac gacgacgatg ccgtccggca
     1921 ggccgccgag ggcgtgtccg aggatcttcg ggtcgatgcg ttcggccacg agtccgggca
     1981 tggccgagcc gccgccgcgg cgtctggcca cagcgcgcgc gagcttgccc gcagcgatcg
     2041 cggctgatgt gcgcagcatt cgagaaccct atgtcacccc gcgaagggct ctcgcgaccc
     2101 cgcctcgccg gagccgcgcg cgtcgagttc ggcgttgacg tcgctggcgt accgcgcgga
     2161 atgctcctgc cccgacatgg ccccgatcga atccatcacg gcatcggtga tcgcccgcag
     2221 cagcgccgcc ttggtgaatt gtgcttgcag cgcagcgaca tcgatcgccg gcccgaactg
     2281 caccgtcacc ttcgtcggcc gcagcactcg cgcgtccggc ggctgcagct tgtcggtgcc
     2341 gatgagcgcg cacggcacga tcggcgctcc cgtcgacagc gccagcctcg cgacccccgt
     2401 gcgtcccttg tgcaggcgtc cgtcccgcga tcgcgtgccc tccggataga tcccgaacgc
     2461 cccgccggca cccagcaccc gctcggccag gtgcagcgac tcggcggcgg cccgcgcgtc
     2521 ggttcgctcg accgggatgg tcccgaacga gcggaaccag gcccgcatca acgcaccctt
     2581 gacccccgtg ccgtggaagt actccgcctt gccgaggaag ctcacccgcc gccccgacgc
     2641 cagcgggatg accatcgaat cgctgaacga caggtggttg ctcgcgatga tcgccgggct
     2701 gccctcgggc ggaatgtgtt cgcgcccaac gacagtcggg cgtgccagcg catagaacgc
     2761 cggcccgatc accacatagc gcatcacgcc gtcgaggagc gcgttgccct cccccaccgg
     2821 gtggccagcg gtcatgtctc cccctccgta tgcggtcggg tggtcgtgtg cggtccggtc
     2881 gccgtatgcg gcgtggtggt cgtaggcggt ccggtcacca ccgccgggac cggctcggcc
     2941 acggcgcggg gcagccgcca caggcgccgg tcggcgagca cggcgatcag cccgaggccg
     3001 aggccgatcg tcaggccgac gacgatcaag gacgcataga ccaaggtggc cgcgcgagcg
     3061 cccgggaccg gctccatgcc gagccggtgc agcacgaagg cgccggcggc gacgccgagc
     3121 acggccagcg cgctgtgccc gaccgcccga tcgaatcccg cgggccggac ctgcggcggt
     3181 tcgacccacc gggcaccgat gagggtcgcc acggccgccc agatcaggac gacaccgacc
     3241 gcagcgatga cgtcggccgg tttgtgccac cgctcggcca tcgtgcccag cccggccccg
     3301 ccggcgatcg cgcccgagag caggcacacc aacggccgca ggatgcgcgg cgcggcgatg
     3361 atggccacgg cggcgagcga gatcgccgcc gtcgagtgcc cgctcggcat gctcacccgc
     3421 acagccggat cgctcggcat atcggtcagg aagcgcagct tcagcagttg ggtggtctgg
     3481 ttggcgccga tgacgagcag cggcagcgcc agggcgacgc gccacagccg ccgggcgagc
     3541 acgacgagca tcgcgatcag cgtgagcaac agcatcgtcg ggatgcgaat ctcctgcagc
     3601 agcgcgctga tccggcccgg acggaactcg tcgacctcga aggaggcgac gacgtcccgg
     3661 tcgagccgga tgccggcgtc cgtgcgcagc gccagcacga cgatgaccga cagcaggacg
     3721 agccctgcaa ggaagacgag gaggacatcg gcggcgtcgc ggaacgcgga gcgcggcggc
     3781 gactgcgggg tcactcccac tcgatcgtcc ccggcggctt gctcgtcaca tccaaggtga
     3841 cccggttgac ctgctcgacc tcgttggtga tccggttcga gatgtgggcc agcacgtcat
     3901 acggcacccg ggtccagtcg gccgtcatcg cgtcctcgga cgagaccggg cgcagcacga
     3961 cggggtggcc gtaggttcgg ccgtcgccct gcacccccac cgagcggacg tcggccagca
     4021 gcacgaccgg gcactgccag atgtcgcggt cgaggccggc cctcgtcagt tcgtggcgca
     4081 cgatggcgtc ggccgcgcgc aaggtggcga ggttgtctgc agtgacctcg ccgacgatcc
     4141 ggatgcccag cccgggaccg gggaagggct ggcgccacac gatcgcctcg ggcacgccga
     4201 gttcgagccc gaccttgcgc acctcgtcct tgaacaggtc gcgcagcggc tccacgagcg
     4261 agaactgcaa gtcgtcgggc agcccgccga cgttgtggtg gctcttgata ttggccgtcc
     4321 ccgacccgcc gccggattcg acgacgtcgg gatagagcgt gccttgcacg aggaactcca
     4381 ccgggtgccc gtcctcggcc gcgtgcccga cgacgtcgcg ggcggcctgc tcgaagacgc
     4441 ggatgaactc gcgcccgatg atcttgcgct tctcctccgg atccctaact cccgcaaggg
     4501 catccaggaa ctgctggcgc gcgtcgacga cgacgaggtt gatgcccgtc gacgcgacga
     4561 actcctcctc gacctgctcc gcctcaccct cgcgcagcag cccgtggtcg acgaagacgc
     4621 acgtgagctg atcgccgaca gccttgtgca ccaatgccgc cgccaccgac gagtccaccc
     4681 cgcccgagag ggcgcacaac acgtgcttgt cccccacctg cgcgcggatc tgcgccacct
     4741 gctcctcgat gaccgacccg gcgtcccagt cgggggtcag ccgcgccccg gcatacagga
     4801 agtgctccag cacctcctga ccgaacgtcg agtgcagcac ctcggggtgc cattgcacgc
     4861 catacagccg gcgctcgtcg tcctcgaagg cggccaccgg cgcccccgcc gtggatgccg
     4921 tcacccgcat ccccgcgggc gcctccgtga ccgagtcgcc gtgactcatc cataccgact
     4981 gctcgcgcgg ctgcccgcgg aagagggtgg actcggtgtc ggccacctca acccgcgtct
     5041 gcccgtactc gcgcaacccg gttcgcgcca ccgtgccccc gcgcgcctgc gccatcgcct
     5101 ggaagccgta acacatgccg aagaccggca cccccgcctc gaggagcgcg ggatcgagcg
     5161 ccggggcccc ctcggcatac acgctcgacg ggccaccgga gaggatgatc gcctgcgggt
     5221 cccgcgcgag gagatcggcc acgctcgccg tatgcggtag cacctcgctg tagatccgtg
     5281 cctcacgcac ccggcgggcg atcagctggg cgtactgcgc gccgaagtcg acgacgatga
     5341 cgggacggcg ggcggtggtc acgccggaat gctagtcgcc gcgaattccg cggcaacctt
     5401 gcgttcgacc cagaaggaca ggaagggcac acatccggcg agcatgatgc cgaccatctt
     5461 cggcagcgac cagccggcct tgaagcccag attggcggtc gcggccacat agacgatgta
     5521 gaggaagccg tgcggctgcg gccaccagtc gagggcgtgg ttgtcgaagc cgtagcgcag
     5581 gatgatctcg gcgaccagca cgagcaggcc gatgccgacg atgaacgcca tgatctcgaa
     5641 gaacttcagt gcgctcgcca gtttgggatc gcggtcggtg gtcggggtgc tcattgctcc
     5701 tgcttgccgg cgctgacgcg cacgtcgggg tcggggtcgg gtcggggctg ggtatgcggt
     5761 ggctcgggtt cgccgtgccg ggcgccaggg ccggccgcat gctcgggcgc agggccggcc
     5821 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagggccg gccgcatgct
     5941 cgggcgcagg gccggccgcg ccccggaccg cagggccgcg ggcgtcggcc tggaccatct
     6001 tcacccacag gaagaaggcg aaggccgcga acacccacca ctgcagcgcg tatgccgcat
     6061 tgcgccactg caccccggtg tccggtcgcg gtgggggcac gcgcgtgagg ggggcgggcg
     6121 cgtcggaagc ctgggggtcg ctcacggggg tctcgcgctg ggcgaacccg aaggcgttgt
     6181 agaggtcacc gggccaggtg ttgacgagtt cggagaggtt gacgctgccg agcgtgcccg
     6241 ccggataggt accctcggcg ggcgactcgc ccggcgcgag gctggcggtg acggtcacct
     6301 caccgccggg cggctccggc aggacggcct gcggggtcca cccgcgcagc accgggaagg
     6361 tcgccccggt ggcggtgagc cgcagcgggg tgatgaccca gtagccgtcc accccaccga
     6421 ggaggcgacc ggcgacgagg acctggcccg cggcataccg tccggtggcg gtgaccaggc
     6481 ggccgcccgc cgccgcgggg aactcggtat gcggcgtgag cagggttgtc agctcggcca
     6541 ccggctgctc gccggccttg gcgatcgcgt cggcgcgcgc cttgtcgtgg gcgacgccga
     6601 gttgccagcg ccccagctgc acgaaggcga tcgcgatcag cgccgcgagc agcaggagcg
     6661 cgagccactt cggcttcacc gcggtccgca acacccgctc agcctacgtc gcgcgcctgg
     6721 ggcgaccgca ccgccatacc gtcgcccgct gcgccgccgc cgggtgaacg gcaggcgtcc
     6781 gcgctaccgc acgtcgatgg acagtggacg atctctcggg cgccgcgacg cgcgaagtgg
     6841 gcaacttgcc ggtgggtagc ctcgcggact ccccgatccg ggcagaaact aaccaagtcc
     6901 taacctgatt tttacccaaa tcgcctggtg ggggcgctgg cgggttgtta ggttcccagg
     6961 caagtcagat cgcgttacac acgagaggga gcagggccct ctcgacctgg acaccgagga
     7021 gggatcgtgc agggggacac cacgtcggcg acgtcgacgc tcagggcgag cgcggcgcac
     7081 cggcaggagc acgaggacct ggcggcccgg gcccgcacgc tgttgaacgt cgatccggag
     7141 catctgcaac cggtggtcat caccggcccc atcggcggac tggtcaactc cgcgatccgg
     7201 cgggtcgcgg cggccagcga cctgcccggc ttctggctgc actcgctgcc gccgcggccc
     7261 gacggggatc agcggtgcat cgtgcaggag gcggcccgcg acggcgagca ggacggcctg
     7321 gccatgacgg ccgacgacat gggcggcctg ctcgatcagc tgacagcgca ggtgcagggg
     7381 cccgcgctgc tcatcgtcga aagcgtccag cagttgtcca cctcctcggc gaatgcgctg
     7441 gtcgtgtggg cccagctggc gcggttgcgg ggcacgcgcc tgctgctcgg gtatgccccg
     7501 gccgccgaca tcgacgcggt cgggacgctg gtcgcgatga acccggaggt gcactggatc
     7561 gacgtgcagt cgccctcccc cgagcggctg cggcacgcac tggagcgggc ggcgcggtgg
     7621 acgggtgcgg acgagctgga ccgcgcggcc gccttctgcg ccggcagtcc cggactgacg
     7681 accgccgtat gccgcgccgc cgcgatcgag ggtgaggtca ccgactcgcg tttcccgcac
     7741 gtctacgcca atgccctgga gagttccgcc gtcccgcagc tgctgcggca gggtcccgat
     7801 gccgtcgccc tggcgttggt catggcgctc gccggtcaca ccgtggacgc ggccgaggca
     7861 gccggtgcct tgggcatcct gccgcgcacg gcgcggcgtc tcgagcggtt cctgcgcgag
     7921 agcggtctgg cgcccgacag cccggaggcg caggtcatcg tgcaccgggt ggtcgctgcc
     7981 caggcgggtc aggacatccg gcgccgggcg gcgagctgtg cgcgcggtct cctcgaggcc
     8041 caccgcgccc aggccgtcga cctgttcgcc ctgcactccc tcgtcggtga cgatgtcgcc
     8101 gggcggatgc tgctggccaa ggacatcttc accgccgccc tcttcgagga cgccttcgac
     8161 cgagccttgc aggtggcgca gaacctcgag gccacatcga gcgatcccgg ggagatcgcc
     8221 tgggcccgcg cggcgcagct ggccgtatgg atgcgctcgg actgggcggc gctgcaccgg
     8281 cagcgcgaat ggctgaccga cccggcggtg ctcgccgaac tcgacggcgc ccggccctcc
     8341 gcgtggctgg ccctcgaatc accggccacg gtcgagttgc tcctcgaagc gccagccgcc
     8401 gacggggcgt ggagcggcct ggacgccacc ttcctttccg gccggatgtc cacggtcccg
     8461 accacctatc tgcgcggtgt ggtcgccgcg gccgagaccg ggcccaccta tgccagctat
     8521 ctcgccgtgt gtgcgctcat cgggcaccgg gcgagccctc ggctggccgc gttgagcgaa
     8581 tgtgccgagg cgctcggtgg gccgctgggc gtcggtgctg cgctcgcgag ccggatcgcc
     8641 gccgcgtggc tcttcctcac cgactacgag gccgctgccc ggtgggcggc catcgcggcg
     8701 ttcaccgcgg ccgagaacga ggccgccgac tgcgcgatgg gctatctcat cggcgcccag
     8761 gcccaattgc gcctcggcaa cttcggacgg gcccgtgagc aggccgaact cgccaagacc
     8821 aacttcgccc gcttcaacgc caccacgatg atggacgtgt gcgtctggac gctggcgcac
     8881 atcgacgccg aggatgtcgc cggcgccgag ccggccgacg cccaggcgtt ggccgcggcc
     8941 ctgccggatg cggtgcaccc gttgtggatc gcctaccgcg cctatgtctg cgggcgcacc
     9001 ctgttgcggc acggcgagac cgacgtggcg gtgcgttatc tcttcggcag cgggcgggac
     9061 ctcgcccgcg ccggcatcga gaacccgtcg ctgctgaact ggctgccgca cctggtgcgc
     9121 gtcttccggg ccggcggact gacggacttc cacaaccagg tgaccgacga cttcgccagc
     9181 cggtggcggc gctgggaccg gatcgagccg gcggcggccg ggcgacgggt gcgggcgctc
     9241 gccgatgtcg ggaccgaact ggggctggtg cccaaggaga gccccaacga gcccggcccc
     9301 tcacagctgt cgccggccga gcgcaaggtc gtcgtcgtgg tcctcgaggg gctgagcaac
     9361 cgtgaggtgg cccgcaagct cttcctgagc aaacgcacga tcgacaccca cctgggcaat
     9421 atctatcgca agctcggcct gtcctcgcgc gacgagttga tctgctgggc ccgccggttc
     9481 ccgctcagca gcgacggggc gagcgaggcg gcatacggct gaccgcctgc cacccctgac
     9541 cgcccgccac ccccttgcca tcacggtcgt ctgcggcccg cagttttccg cccaatgcca
     9601 cttcatagcg gtgcgcctcg caacgttcgc gaggcgcacc gctatgaact ctcccagagc
     9661 cacgtgctcg cgggtcaggc gccgacgaat ccggcgatga gcccggcgct cacctcgggg
     9721 cgttccagca gcggcaggtg cccggcgccc tcgatgatct tgacctgggc cgccgggatc
     9781 gcctgggcga tggtgtccgt gttcgcaatg aactggggca tgtcgtcggt gccgacgatc
     9841 agcagcacct cggcccggat ccgcccgagc acggcggcgt cctggacgtg actggacaag
     9901 gtggccatcg cgccggagcg cagctcgccg aaggggtgtc cggcgatgat gtcggccatc
     9961 gcggcatacc gctgcgggaa ctcgcggagg ccggtgaaga tccgcggggg gtcggtcatc
    10021 cagatccggg cgatgtcggg gccgggtccg agttcgccga tgcgcatggc gagcatccag
    10081 tatttcgccc gtgccgccgg gtcgtccggg acaccggaga gggtcggcgc tgccaggacg
    10141 aggcggcgca cgagatcggg atggtcggcc gccacctgca aggcgatgct cgaaccgaag
    10201 gacagcccga cgagttcggt gctgccggcc gctcgcatct cggcagccac catgtcggtc
    10261 cagccggaca tgctgcgcgg catcggggtc ttcgccttgg atccgtggcc gggcaggtcg
    10321 atcccgaggt ggtcgcggcc gggcaggagg tcccacagcg gctcccagac ccgcgacgac
    10381 atcgtgtagc cgtgcagcca ggcgatcggg ccggtcatgc ggaggctcct gacggtgccg
    10441 gggccgggct ggcggccggc gggaagtcgg ccacgaaccg gcggaaggcg atgactccgg
    10501 cgtcgagatc gtccagttgc cacggagcgt cggggtcgat gtgtttccag accgcgatgt
    10561 cctgggcgat ggcgatcttg gcgccgacgg cgatgctgtc gagcgggtcg tgcgcgccgc
    10621 cgacgatcgc gacccggatg tgcacgccct cgctcgtcgg ggtcgccatg gtgacgatca
    10681 acggcggccg gggtccctcg ccgaacgcgg tggagacgat cgtcgggctg tatgccgtcg
    10741 cctcgaatcc cgaactgcgc gtaggagttc cgcggtagtc gctgacgaag tcgttcagtg
    10801 ccgccgcata gcgcatgtcg taccacgggt cggccatggt gcggaagtcg ccactgatcc
    10861 gcaaggctcc ggagggcagg gccgtcgtgg tcatggggcc gaggccgggc acttggtgca
    10921 ctgcgctgaa gtggtcggag tcgaaggcgt tctcgacgac gaactccacc ggcatgtcga
    10981 ccaccgcgtg caccgcgacg cggatctcgc ggtctgcgac gaggccggcg aagtcgacgg
    11041 agaagtcgca gtccccgacc ggatcggtgg cgaaccgggc gaagacgagg ggccccacct
    11101 ggagggtcgg gtgcgtgctg acgaagggcc gcgtcgggtg ttcaccgacg acgatccggc
    11161 ggccgtggaa ggggcagacg acgcaatccc ggtccagcac accgccatag ccaaggtgag
    11221 cgcctcggtg cggacacgtg gcgtcggcga ccgtgaaaat ggcgtcaccg tcgtcgctgg
    11281 cggtgcgggt caccatgagc cggcggccac cgacgtcgag tggggtgata tcggcggtca
    11341 gctcgtcctc ggtggcgacg agataccacc cggcgtgcag ggtctggttc atcgggacaa
    11401 ctcctccagg atcgcgtcga cggcgcggcg gccgctgtgc caggcgcggt cgacgtacgg
    11461 atgcccttcg tcggcccacg ctgcttctcc cgccatgtgg aggcgcccgc ccacgggcgt
    11521 ccgccactgc cgcgcagcgt cgccggcggg gtcgaccggt gcggtgaatc cgccgccggc
    11581 cagcgggtcg tcgtgccact cacggacgat cacctcgcgg cgattgtcgg gctggactcc
    11641 aagcgcggta acggttttcg cccaagccgc gcggtcgcgg gtccagccgc gcaggcggta
    11701 tgccggggcg cccttcgcca cgagggtcgc gtgttcctgt ccgtcgttgg ccgtcaggaa
    11761 accgacgccg gggacgtagg cgaagctgtc cccggccgct gcctcaccga gagggaggat
    11821 cgccacggct gcgggtatgg cgcgcagttg gtctcctgcg gcgcgctgcg cggccggcat
    11881 gtcaggcagg tcgatggcgt gagcgacgct gggcggcgcg gtgaggatga cgtgacgcgc
    11941 gcggatctgt cctgtctcgc tccggacgac gacacctgcg ccgtcgatac tgatgtgccg
    12001 caccgtcgtt cgcagggtga cgggcagctg ggccgcgacc ttctcgagcg gggtcgtcat
    12061 gccgcccggc agcagatgct tctcccccgg tggtggtcgc accgcggcgt ccgtcaggta
    12121 gcgcgcgaga gtctcagctc cggggtcgat gccggcgatc tgctcggtcc agccgcgcaa
    12181 caggtcgccg gtcgccacgc tggctccggc gatgcgcacc gcgccggtca ccgccagccg
    12241 ctcggccatg aaggccccga ggctgcgata cacgcccgcg atcagccgct ctgcacgcag
    12301 cggtccggtg ggatccccct cgcagtccaa cggctgcagg gtctcgccgt cgagccagcc
    12361 ggccgcgatc tgggcgccgt cgacgaggcg cacggtcggg gccgcgccgg cccacgacag
    12421 gaacgggttg tcgctgccgt ggacgacctg cgcgccgagt tcggcggcgc ggccggcgtg
    12481 ggtgaccgtc gcgacgcgtc cccccacgcg gtcggcagcc tcgagcacct cgacgcgcag
    12541 accgctcgcc acggcatact ctgcggccgc aaggccggcc accccggccc cgacgacgac
    12601 gacgtccatc tgggtcacgt cgccactccg ccgtgcacga tcgcggacat gggccggcgc
    12661 gggaaaatgc ctggccgcca gacgaacagg ctcgaggcga cacgggctcc cggacgttgg
    12721 gaccggagcg agcggatcgc gagcgggacg acggcgcgca ccagagccgt ccccaggcag
    12781 acatgagccc catggccgaa ggccagatgc gcgctggcgg ccggccgctg cgcgtcgaca
    12841 cgatccgggt cggggaaccg gcggccatca cgattcgccg acccgagatg aatgaggact
    12901 tgcgcccccg ccgggatggc cccggacggc accgtcaacg cgctgatggt gcggcgcgcg
    12961 acgaacggga tcggcgtatg ccagcggatc acgtcgtcgc accacggctc caggtccgcg
    13021 tccgccggac cgtcgtcacg caaggccagt tccagcgccg tcgtcacgac ctccgccagc
    13081 ggcgaccagc cgccgacgac ggccagcgag acgatcccgc gggcttgttc gggcgtgatg
    13141 ccgaatcgcg atggcaactg tgccagaagc gaattgggcg ccgtggcgac gagccgctcg
    13201 acatagccgt cgaactcgcc catcgccaag gcgccctgcg cggcctgggc cggtgacggc
    13261 atggggtcga tcgcgacgct cacggcccgg gcgtgccggt cgaagtcacc gaatcgggcg
    13321 tggtcggcca ggccgaggat ctccagcagc gccagcgtcg ccagcggctc ggcatacgcg
    13381 ctcgtgatgt cgaagcgggc gggcaactcg gagacgagat tgccggcagc ggcggcgagc
    13441 gccccggagg acgcgcgcac cgcggccggc ccgagcagcg ccgcgccggg gcgacgcagc
    13501 gaatcgtgtt ctgcgccgtc ggtattgagc atcgacaccg gcgctcccga ccgccggcta
    13561 cgctcttcct ggccacccgc cgccgaacag gccgggtcgc gcagggcagc ttcggcgtcg
    13621 gccgcgcggg tgagcacata gaccggctcg ggtcccggca agcggtggac cggatcgtgc
    13681 tcgcgcaggt gactcagcgt gggttcgggg ttgatcgcga agagcggaga gaagatgggt
    13741 gcgacgccgg ggggtgccgc ggggctgtcg gtcgtggtcg tcatcagcgc accttcgggc
    13801 ctcgccactt gaaggtgatc gccgtcagga agccggccag cagcacccag aaaccgagcg
    13861 ccatcgcgat gagggggagg ttccacaccc cgcccggctc gaccgcggag aacccgtcgg
    13921 gcaggaagac ataacgcagc ccttgggtca tccacttcag cgggaagatc ccggccgtcg
    13981 tctgcatcca ctccggcagt tggttgaagg ggaagaagac accggagatg aactgcagca
    14041 ccaggaacgg tggggtgacg atggccgcgg cgctgttggc ggacgggatc aacgcggtgt
    14101 aggcgaggcc gctgagcgcg caggcagccg tgccgaggac gaagatccac ccgaaggcga
    14161 tccagtggtc ggcgtcgctc ggcaggtcca gtccgaacag ggcggtgccg agcgcgatga
    14221 ggatcagcgc ctccaggagg gaggtgacga gcaccagcac gaccttgccg acgaagtagc
    14281 tggccttggg catgggcgac ccggcgagcc ggcggatcag acccatctcc cgttcgaggg
    14341 cgacattgat ggccaggccg gtgaaggcgg tgctcatgat gcccgcggcg acgatcccgg
    14401 cgacgaagta ctgcttgaag tcggtgtcgg tcccctcgat cttgccgctg aagatggatc
    14461 cgaagatcat cagcaggatc accggcagcg cgagggtgaa cacgaccgat tgcgggttgc
    14521 ggaagaagac gcgcaactcg cttacggagc gcgcgagact ggtgcgccac aacccgggga
    14581 ggggccgatg tgcgggcgcg ggtggagccg aggacgcggt ggtcgtcggg cggctggagg
    14641 tcatcgtcat cgctgggcgt cctctcgctg gatcaaccgc aggtaggtgt cttccaaggt
    14701 gggccggctg acggtcaggc cggggatctc gccgaaaccc tgttcgcggg ccaggcgggt
    14761 gagccgttcg acgacggcgg tgggctggtc ggtgccgatg agcaccccgc cgcgccggtc
    14821 cgcccgggcg tcgggttcga ggacgccgag gtcggacggt atgacgcccg ccaccccctc
    14881 gagatcggtg acggtgacat tggcgctgta gccggcggtg cggccgagtt cgtcgatcgt
    14941 gccgatcgcg agcatccgtc cggcggcgat gaccccggca cggtcggcga gggtctcggc
    15001 ctcgtcgagg tagtgcgtgg tcaagacggt ggtggcgccg agttcgcgga agtagtcgac
    15061 gaggtcccag gcctcccgcc gcgcctgcgg gtccaggccg gtggtcggct cgtcgaggaa
    15121 gatcagttcc ggcttgccga ccaccccgac cgcgacgtcg agccggcgct tctgcccgcc
    15181 ggagagcgca ttcgtcttcg cgccggcctt cgcggtcagg ccgaccatgg agatcagttc
    15241 gtcggtgggg atgggatcgg agtagaaggt ggcgaacatc tcgaccactt cacggacggt
    15301 gaggttgagg tagtcgccgg tgttctgcgg cacgacgccc aggcggttgc gccactggcg
    15361 gctgtcgtga tgggggtcct cccccagcac gctgacggtg ccaccggtgc ggcgctgcac
    15421 gccgcagagc atgtcgaccg tggtggtctt gccggctccg ttgggcccga ggagggcgaa
    15481 gacctcgccg ggccggacct gcaggtcgag atggtccacg gccgcgaagt caccgtaggt
    15541 cttgcgcaag ccctgcacgt cgatggggag gtgatcggtg atcgttgtca tgttccactc
    15601 cttccggtgg catcagtctt ggcgggcctt ggcggtggta acaccccgcg cacctactca
    15661 tcccccgggg tgtcgtacgt aaatggatgc gcactgtgcc ctccggggac agcgacgggc
    15721 gtgaggcgct gatgcgcctc acgcccgggt gcgtggctgg tcgtcagcag cagcagcagc
    15781 agcacggaac ttcgccggca gccacttcgc tcaggtcgct ctcggagagg atgccctcct
    15841 cggtggacgg cgcggccggc aggtggatct gcaccttgcc ggacttcagg ccggcctcga
    15901 agatcgcctc ctgggcgtcg agggcggctt cgttcgactt cccctcgtag gggttgacct
    15961 cgccctggcc gtgccggatg acctcgacgt cgacgccatc ggggatgacg atgccgacct
    16021 cggcaagggc acccttggcg tcggcgtcga cgcgggcccc gaagccctcg tccgtccagt
    16081 ccttgacgag gagctgaagg tactgctcgc ggaactcgtt gctgctcata gtttttctcc
    16141 ttattggttg gtttcgttcg aaggtgaacg gttgggggtg gttggtgtca gatcgtgcag
    16201 gagctgcagg ccgggcagca gcagcagcag taggtcgtgc cggcggcgat gacgccgagt
    16261 tcctcggcgt tgagatcggc cgtgtcgacg accggggcgg tcggcatgtg gaagacgaag
    16321 tggccgcttt cgagaccggc ctcgtagagg gcgacctgct tgtcgacgtc tccgtcccgg
    16381 cgggcctcgc ccacctggtc gggcgtgtga cggatgatct cgatcgtggc gccgggggga
    16441 agttccatgc cgaccgacac gagcgcctca cgcgggttgc ggtcgagctg accggcgaag
    16501 tcctcgtcgc accagctctg gagcaaggcc tgcgagtagg acttgatgaa ctgggtccgg
    16561 gcggcttgtc gcatgaccag cccttagccg cagcagcagc aggggcagca gcagcaggcg
    16621 atcgtcagac cgccggcgac gcccgccagc tcgtcgatgt cgatctcgaa gatgtcgatc
    16681 tggaaagtcg acggcatgtg gaactcgaaa cgcccggact ccagcccggc ctcgtagagt
//
LOCUS       DAOPJG010000059        16573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3681_length_16573_cov_8.173447, whole genome shotgun sequence.
ACCESSION   DAOPJG010000059 DAOPJG010000000
VERSION     DAOPJG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16573
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_16573_cov_8.173447"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1225)
                     /locus_tag="PKU75_07375"
     CDS             complement(236..1225)
                     /locus_tag="PKU75_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80734.1"
                     /translation="MRRSATALAAATVTLTAGAAAATTANAQPAATTAASVSTAAAPG
                     TRADAAYLTILFGRTQRQSMDRGCEPLPNSVPLTRVATVMRDRGVSGVGNVVVKRTGQ
                     TPYCFENYVETTSWNDLTQLRTNYGWTFTSAGMNYLNMTQLTRAEQHAESCGSVGALA
                     RRGHTRAWGLFAYPNNKLNDTIQAQVVKKCFAFGRKYGTGLTSKAAGTKWPNWQSTLS
                     VEGGKCADPTQPCNSLLYKSIYMTPAKLIPKVKVNPGYWSTLQFYRFVTGTSSPGTAM
                     QWNCSGPENTHWTSDAELYCIRDFIRVIDAIPDTVRVTDPATVAEAWGVKPSA"
     gene            1379..2311
                     /gene="coaE"
                     /locus_tag="PKU75_07380"
     CDS             1379..2311
                     /gene="coaE"
                     /locus_tag="PKU75_07380"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPF80735.1"
                     /translation="MLRIGVTGGIGSGKSSIAAVLRDRGAVIIDADAISRQVVAPGSA
                     GLAGVLKHFGPGVRAADGSLDRAALARLVFTDPGARRELEAITHPLIWAEAGRQFAAV
                     PADGVAVHDMPLLVEKRMSGEYHLVIIAMVDPETRVRRLVEQRGLDEADARARIAAQV
                     SDDERVAAGDVLLDNGGTPDDLTAATGRLWQERIEPFRANLLALRPAAPATSPLELAG
                     AQAQAGRIATRIAHTITDRIEAIGARVTSVAGGLLTIDCGVVAAKPAGDGPWLAPEHR
                     AALTHAGFPGVAGGDVFGNCDPAWPSRITLRRMP"
     gene            complement(2385..3374)
                     /locus_tag="PKU75_07385"
     CDS             complement(2385..3374)
                     /locus_tag="PKU75_07385"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HPF80736.1"
                     /translation="MTTPPLDDLLARLHVVAIPMRVPFRGVTTREVALVEGPAGWGEF
                     GPFLEYEPPEAARWLAAAIESGYDEWPAPQRTAVPVNATVPAVAAQRVPGILARFPGC
                     DTVKVKIGEPGQGRSDDVDRVAAVRDHLGPAGRIRVDANGAFDVAAATGLLARLAPYG
                     IDYIEQPCATVPELRDVRRALARGGLDIKVAADESIRKAEDPLRVARQQAADLVVVKV
                     APLGGVRRALDIVDACGLPAVVSSALDTSVGIAAGVALAAALPDLYGACGLGTLNLMA
                     GDVTSDPLLPRDGTLPVCRPVPSAELLQEYAVAPAREKWWRERVAACYPLLPA"
     gene            complement(3371..4420)
                     /locus_tag="PKU75_07390"
     CDS             complement(3371..4420)
                     /locus_tag="PKU75_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HPF80737.1"
                     /translation="MTALRRLHAAYRASGADLPFGDPLSAHGLAMEGYFWRISERASG
                     RAAIVLAGINRPHDGTAPWSTLGIALSPGGAISTLAHEQGYADPHRLGAAAGAAFRAD
                     AGRVHAEWAGASADFVVAQPRPWPLRPFGGSSIFQSVPGLNQYWHPWLLGGQASGVVT
                     AGDDRWEFTDAQVYGEKNWGKGGFPDSWWWGQAQAFAEQDACVAFAGGQVYAGPIRTT
                     VTGLVVRLPGGRVVRLGNPGLSPVRAQVDDTTWRLRGRGYGWRVDVHAAAPLGDAHVL
                     PVPLPDERRNTAGALEHLAGTLSVTVRRRGRLVWSGESPLAGLEHGGLARAEAELRRR
                     GIPPGATDAPPQVRP"
     gene            complement(4417..5058)
                     /locus_tag="PKU75_07395"
     CDS             complement(4417..5058)
                     /locus_tag="PKU75_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010143549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPF80738.1"
                     /translation="MPHGPQEVGLGPWVGEWPNGPGAEVYDESLLREGDRRNVVDKYR
                     YWSMAAIVADLDSRRHDFHVAIENWQHDFNIGTIVRTANAFLAKEVHIVGNRRWNRRG
                     AMVTDRYQHLRYHADATELAGYLHERDVRLWGVDNLPGSLHLEDTPLPRRVCFLFGQE
                     GPGLSEQARNACAATFSIAQFGSTRSINASVAAAVAMHAWVREHADFSEAWRG"
     gene            complement(5088..5480)
                     /locus_tag="PKU75_07400"
     CDS             complement(5088..5480)
                     /locus_tag="PKU75_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HPF80739.1"
                     /translation="MTTARNVLGGPLAECGTDPMTGFFRNGACWTSPEDFGRHTICAV
                     MTPEFLDHQQRTGNDLITPIPAYRFPGLQPGDTWCVVALRWLHAHQDGAAAPVILAAT
                     HEATLEVIPLEVLLPYAADAPDDLSALQ"
     gene            complement(5616..6302)
                     /locus_tag="PKU75_07405"
     CDS             complement(5616..6302)
                     /locus_tag="PKU75_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80740.1"
                     /translation="MLPSPVERLLGLVPGVAIAGATSALALLRDRKPLHPKGVVGRGV
                     LDISGAAALRVPVLDTPGSYEVTARFSWAIGVGPERADIHGLAVRFEAEGALVDWLLA
                     STGAGGLDRFVLQTREPGTFGTLTTLLPVDSPAGPLLLRAEPEGHGEGLLPPAEHQLY
                     AALGRGGWQPIGRLGVAWSDHDERIRFDAVAHPTPGAGSYPLVTTLRTPAYRLARLLR
                     PNPKDPAPLG"
     gene            6369..8477
                     /gene="uvrB"
                     /locus_tag="PKU75_07410"
     CDS             6369..8477
                     /gene="uvrB"
                     /locus_tag="PKU75_07410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPF80741.1"
                     /translation="MRPVTELKRRVAPFEVVSEFEPSGDQPQAIGDLATRVKAGEQNV
                     VLMGATGTGKSATTAWLIEQVQRPTLVMAPNKTLAAQLANEFRELLPHNAVEYFVSYY
                     DYYQPEAYVPQTDTYIEKDSSINDEVERLRHSATNSLLTRRDVVVVASVSCIYGLGTP
                     QEYVDRMVPLKVGDVIERDQLLLKFVQMQYTRNDLAFTRGTFRVRGDTVEIIPVYEEL
                     AIRIEFFGDEIERIYTLHPLTGEVVREEEEMYVFPATHYVAGPERMERAISGIEKELE
                     HQLATFEKQGKLLEAQRLRMRTTYDIEMMRQIGSCSGIENYSMHIDGRSPGSAPNCLL
                     DYFPDDFLLVIDESHQTVPQIGAMYEGDMSRKRTLVEHGFRLPSAMDNRPLMWEEFLD
                     RIGQTVYLSATPGNYELAKADGVVEQVIRPTGLVDPEVILKPTRGQIDDLLDQINQRA
                     ARNERVLVTTLTKKMAEDLTDYLLEKGVRVRYLHSDIDTLRRVELLRELRLGEFDVLV
                     GINLLREGLDLPEVSLVSILDADKEGFLRSARSLIQTIGRAARNVSGQVHMYADQLTP
                     SMQEAIEETNRRREKQVAYNLEHGIDPQPLRKKIADITDMLGREDADTDTLLGSGRTQ
                     SRGTRGAGRGGRGAMMADTGQVATIKGLPASDLANLIQELTEQMHQAAAELKFEVAAR
                     LRDEVKDLKKELRQMSEATR"
     gene            8516..8857
                     /locus_tag="PKU75_07415"
     CDS             8516..8857
                     /locus_tag="PKU75_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPF80742.1"
                     /translation="MTPEEVRDHALGKPGAHPDEPWEGDHVAKVVEKIFVFGLGGDSV
                     GVKCGANRAEADEWLHQYPDDATVMAYIGRNGWNTLRIGGAIPDGEILEAIDTSYDLI
                     VSKLPKSKRPS"
     gene            complement(9046..10812)
                     /locus_tag="PKU75_07420"
     CDS             complement(9046..10812)
                     /locus_tag="PKU75_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HPF80743.1"
                     /translation="MRRFRMSVLSACVLSAALALLGCGGSSAVAPVAPMASAPATGVS
                     VSSQAGARSLSFRIERLAGADRYAAAAAISREFQPGTSHWVVIASGELWSDGVAAGPV
                     AAKLGAPVLFVTKYSVPKATAQELDRLRPSQILVLGGRATISDEVMVDLSAWTDEGAE
                     RLDGADRFAVAAKASAEVFTGTVETAYVASGRVWPDALTGGAAAAVQGSPMLLTEPDR
                     VPAVIATELRRLAPKRIFLLGGTASVDAGVAADLADIAPVERLWGDDRYDTALAISKR
                     IFGTDRPAMMIATGANYPDALAGSAQATRTRGPILLSRRDTLPSGTSDELTRLSPDTA
                     YILGGPASVPINVPRLVQKELGVCWAGKPPVNTDVEAVKKIYGMTVQQIAYTLDMGGR
                     LDGGADIVRYLIDNQVCTTFFPTSIMADTSEGREILGLIAAHPELFEVGNHTVHHCDL
                     VNGGGGSPTSAPCQRSMTDSFIRSELSDAESVLKRLSGMPIRPYWRPPFGVYDSHVQS
                     VVAGVGYPKTMMWSRDTIDWDPATTTQDIIDAVLDPTPETGTIVLSHLGGYHTGEALK
                     TIVPTLRSRGMTLTTISDMWDH"
     gene            11280..12287
                     /locus_tag="PKU75_07425"
     CDS             11280..12287
                     /locus_tag="PKU75_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPF80744.1"
                     /translation="MLAAANETQLNLPTWAWWLTIGIAIAVLAFDVLWIMRNPHRPST
                     KELVVALSIYIGAAVAFGIALLVTKGGKLSGEFFAGWLTEYSLSVDNLFIFLLIMANF
                     AVPERLQQFALMIGIVIAIILRGLFIWAGAAAINNFSWVFYIFGAFLIYTAWKLASGG
                     ESDDDEYEENKFMKFVEKRIPSTKEYHDTKLFSRVNARRVATPMLFVILALGTTDLLF
                     ALDSIPAIYGLTKEPYIVLVANLFALMGLRQLYFLLGGLLQKLVYLSIGLAVLLAFIG
                     VKLLLHALHENELPFINGGEHVSVPEIPIAVSLGAIVVILGVTTIASLWKSKRDARLE
                     A"
     gene            complement(12340..13215)
                     /locus_tag="PKU75_07430"
     CDS             complement(12340..13215)
                     /locus_tag="PKU75_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPF80745.1"
                     /translation="MTAADRVPAPLLVLAGIVSVQFGGALAATLIPHIGAAGSVTMRL
                     LFASVILCAVVRPRLRGYSAAAWRTVILFGLALGLMNFAFYGSLAHLPIGVAVTIEFI
                     GPLLLSAVLSRRPSDYVAVAGAAVGVVLISEALHVPLDQLSWAGILLALTAGACWAAY
                     IILSGRTGAAFPKLDGLAIAMLIATCFVAPFGLATVGDWSAPLLAKGLGIAVLSSLLP
                     YSLELIALRRMSAAVFGILLSLEPACAALAGLLVLGQRLHVWQLLGMALVVGASILIM
                     GGGGTEKDPAEAAAT"
     gene            13329..13514
                     /locus_tag="PKU75_07435"
                     /pseudo
     CDS             13329..13514
                     /locus_tag="PKU75_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491705.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
     gene            13517..13774
                     /locus_tag="PKU75_07440"
     CDS             13517..13774
                     /locus_tag="PKU75_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HPF80746.1"
                     /translation="MLLVWDENAWEDYRWWQRQDRQVRKRINLLLTDITRNGNEGIGK
                     PEALKHDFAGCWSRRITDEHRLVYKIDGDQVRIAACRYHYG"
     gene            complement(13800..14477)
                     /locus_tag="PKU75_07445"
     CDS             complement(13800..14477)
                     /locus_tag="PKU75_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF80747.1"
                     /translation="MTPGSTDYTGVVDPGGPSDVRELDRATIRKMSVSPMHNNVYLIT
                     CRATGEQLLIDAADDADRIMSFIAEGTGAASHLVTTHQHWDHVRALEDVARATAATTY
                     AGEEDADALPVAPGVRLRQGDTITVGEVTLDVIHLRGHTPGGVALAYADPGGITHLFT
                     GDSLFPGGVGNTKLPGQSFESLYADVTERIFDVYPDDTWFYPGHGNDSTIGAERPHLE
                     EWRARGW"
     gene            complement(14474..15061)
                     /locus_tag="PKU75_07450"
     CDS             complement(14474..15061)
                     /locus_tag="PKU75_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HPF80748.1"
                     /translation="MNETSPIDTVLAAVARHTAAVDDAAADLDLTQPSLCTGWTRAHI
                     IAHLGHNAEGLLRLVRAACDDSGETMYASVADRDADIDRSAQLPPDELRAFARDQGAA
                     LVAALPRLAAADPAREVERVPGGPTFQAGNLPGMRLRELIYHHVDLDAGFGFADVEPA
                     LLRRLWEQEIGMVDTSDPAALGTALTRLARGMDPR"
     gene            complement(15100..>16573)
                     /locus_tag="PKU75_07455"
     CDS             complement(15100..>16573)
                     /locus_tag="PKU75_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF80749.1"
                     /translation="RLRDLGNTLIVVEHDEDTIEHADWVVDIGPGAGEHGGHVVHCGP
                     VTDLLNHPDSITGQYLSGRLEIPAPPVRRPIDRQRMVKVVGAKEHNLDDVTVSFPLGT
                     FVAVTGVSGSGKSTLVNDILYNVLANKLNGARHVPGRHKSVTGLEHLDKVVHVDQSPI
                     GRTPRSNPATYTGVFDHIRKLFATTTEAKIRGYQQGRFSFNVKGGRCEACSGDGTIKI
                     EMNFLPDVYVPCEVCHGARYNRETLEVHFKGKTIADVLNMPIEEAEEFFAAVPAIARH
                     MSTLNAVGLGYVRLGQPATTLSGGEAQRVKLASELQKRSTGRTIYVLDEPTTGLHFED
                     IRKLLIVLQGLVDKGNTVIVIEHNLDVIKNADWIIDMGPEGGSGGGRVIAEGTPEDVA
                     ADQDSHTGRFLAPILERSARTPHRASATVQATAKKTTATKTTATKATATKATAQKATA
                     QKATAQRAPAKKVAAKRVVPPKVEVSKAASAAARRARKAG"
BASE COUNT         2595 a         5642 c         5784 g         2552 t
ORIGIN      
        1 ctcgtcctac tcctcgacca cgagcgagtc caccacctcg gctgccgccc cggtcgccga
       61 aggcaccctg gcctccgacg aggccctggc cgcgcttcgc gagaagctca ccggtcagta
      121 ggaccccgct ccacggcata cgacgaatcc cccggtcacc agctgatggt gaccggggga
      181 ttcgtcgtat gccgtggtgg gtcggatgcc gagccggccg gctgccggtg ccgcgtcagg
      241 cgctcggctt cacgccccac gcctcggcca ccgtcgccgg atcggtcacc ctcacggtgt
      301 ccgggatggc gtcgatgacg cggatgaagt cgcggatgca gtacagctcg gcgtcggagg
      361 tccagtgcgt gttctccggg ccggagcagt tccactgcat cgcggtgccg ggagagctgg
      421 tgccggtgac gaagcggtag aactgcaggg tcgaccaata acccgggttg accttgacct
      481 tggggatcag cttcgccggg gtcatgtaga tcgacttgta gagcaaggag ttgcacggct
      541 gcgtcgggtc ggcgcacttg ccgccctcga cggagagggt ggactgccag ttcggccatt
      601 tggtccctgc ggccttactg gtcaggccag tgccgtactt gcggccgaag gcgaagcact
      661 tcttgaccac ctgggcctgg atggtgtcgt tgagcttgtt gttggggtag gcgaacaggc
      721 cccacgcgcg agtgtgcccg cgccgcgcca acgccccgac cgaaccgcag gactcggcgt
      781 gctgctcggc tcgcgtcagc tgggtcatgt tcaggtagtt catgccggcg ctggtgaagg
      841 tccagccgta gttcgtccgc agctgggtca ggtcgttcca cgacgtggtc tcgacataat
      901 tctcgaagca gtacggcgtt tgccccgtgc gcttgacgac gacattgccg acgccgctca
      961 cgccgcgatc gcgcatcacc gtggccaccc gagtcagcgg gacggagttc ggcaggggct
     1021 cgcatcccct gtccatcgac tgccgctggg tgcgcccgaa caggatcgtc aggtatgccg
     1081 cgtcggcccg ggtgccgggg gcggctgcgg tgctcaccga ggcggcagtc gtggcggcgg
     1141 gctgggcgtt cgccgtcgtt gcggctgcgg ctcccgcagt tagggtcacg gttgcggcgg
     1201 cgagggcggt ggccgatctg cgcattgctg aagtactcct tggccggcgg ttcccgggtc
     1261 cgggcccacg ggagccgcgc catcgtgcga gcgggttact cgggcagtgt gccatcgtga
     1321 ccagcggatt gccagccagg gtgtcttgtg ccacgccgtg gatcactact gtgtgcacat
     1381 gctgcggatc ggtgtcaccg gcgggatcgg ctcgggcaag tcgagcattg cggcagtgct
     1441 gcgtgatcgc ggcgcagtca tcatcgacgc cgacgccatc tcccggcagg tggtcgcccc
     1501 cggaagcgcc ggtctcgccg gggtgctgaa gcatttcggc cccggtgtgc gagccgccga
     1561 cggctcgctg gaccgggccg cgctggcccg gctggtgttc accgatcccg gggcgcgccg
     1621 ggagttggag gcgatcacgc acccgctgat ctgggccgag gcgggacggc agttcgccgc
     1681 ggtgcccgcg gacggcgttg ccgtccatga catgcccttg ctggtggaga agcgcatgtc
     1741 gggggagtac cacctcgtga tcatcgccat ggtcgatccc gagacgcgtg tgcgccgcct
     1801 cgtcgaacag cggggactgg acgaggccga cgctcgcgcc cggatcgccg cccaggtcag
     1861 tgacgacgag cgggtggcgg cgggtgatgt cctcctggac aacggcggca cgcccgacga
     1921 cctgaccgcg gccaccggcc ggctctggca ggagcggatc gagcccttcc gggccaacct
     1981 gctcgcgctg cgcccggccg cccccgccac ctccccgttg gaattggcgg gagcgcaggc
     2041 gcaggcgggt cgcatcgcga cgcggatcgc ccacacgatc accgaccgca tcgaagccat
     2101 cggcgcgcga gtcacctccg tggccggggg cctgctcacc atcgactgcg gcgtggtcgc
     2161 cgcgaaaccg gccggagacg gtccgtggct ggctcccgaa caccgcgccg ccctcaccca
     2221 cgccggcttc cccggcgtcg ccggcggcga cgtgttcggc aactgcgacc cggcctggcc
     2281 ctcccggatc accctgcgcc gcatgccgta acccctcccc gtccaaaccc tccgcgtcgt
     2341 gagggcacga ctcccggcgg gacgggtggg acgccgggtg cccgtcaagc cggcagcagc
     2401 ggataacagg cagcgacccg ctcgcgccac cacttctcgc gggcgggggc gacggcatac
     2461 tcctgcagca gttccgcgga cgggaccggg cggcataccg ggagcgtgcc gtcccgggga
     2521 agtagcgggt cagacgtgac gtcgccggcc atcaggttga gcgtgccgag accacacgcg
     2581 ccatacagat ccggcagggc ggcggcgagg gccacgccgg cggcgatgcc gaccgacgtg
     2641 tcgagcgcgg aggagactac cgccggcaag ccgcacgcgt ccacgatgtc cagcgcgcgg
     2701 cgcacgccac cgaggggcgc gaccttgacg acgacgaggt cggccgcctg ctgcctcgcc
     2761 acacgcagcg gatcctccgc cttgcggatc gactcatcgg ccgcgacctt gatgtcgagg
     2821 ccgcctcgcg ccagcgcccg ccggacgtca cgcagttcgg ggacggtggc gcacggctgc
     2881 tcgatgtagt cgatgccgta gggagcgagc cgggccagca gaccggtggc cgccgcgacg
     2941 tcgaaggcgc cattggcgtc gacgcggatc cgccccgctg ggccgaggtg gtcacggacg
     3001 gcggcgaccc ggtcgacgtc gtcgctgcgc ccctgaccgg gttcgccgat cttgaccttc
     3061 accgtgtcgc accccgggaa acgcgccagg atccccggaa ctcgttgtgc cgcaacggca
     3121 ggcaccgtcg cgttgacggg gacagcggta cgctgcgggg ccggccactc gtcatacccc
     3181 gactcgatcg ccgctgcgag ccagcgggcg gcctccggcg gttcgtattc gaggaaggga
     3241 ccgaattcgc cccaccccgc cggcccctcg acgagggcga cctcgcgcgt cgtcacgccg
     3301 cggaagggca cccgcatggg gatggcgacg acgtgaagcc gggcgagcag gtcgtccaag
     3361 ggcggggtcg tcatggccgg acctgtggcg gcgcgtcggt cgcgcccggt ggtatgccgc
     3421 gccggcgcag ctccgcctcg gcccgcgcca ggccgccgtg ctcgagtccg gccaggggcg
     3481 actcgccgga ccagaccagg cgcccgcggc ggcggacggt gacgctcagc gtgccggcga
     3541 ggtgctcgag ggccccggcg gtgttgcggc gctcgtcggg gaggggcacg ggcagcacgt
     3601 gcgcgtcgcc gagcggcgcc gcggcgtgca cgtccacccg ccacccgtag ccgcgcccgc
     3661 gcagccgcca ggtggtgtcg tccacctgcg cgcggacggg tgacagaccg gggttgccca
     3721 gccgcacgac gcggccgccc ggcagccgca cgacgagccc ggtgacggtc gtacggatcg
     3781 gcccggcata cacctgaccg ccggcgaagg cgacgcacgc gtcctgctcg gcgaacgcct
     3841 gcgcctgccc ccaccaccag gagtcgggga atccgccctt gccccagttc ttttcgccgt
     3901 acacctgtgc gtccgtgaac tcccaccggt cgtcaccggc cgtgacgaca ccgctcgcct
     3961 ggccgccgag gagccacggg tgccagtact ggttcaggcc cggcacgctc tggaagatgc
     4021 tcgacccgcc gaagggccga agtggccagg gacgtggttg cgccacaacg aagtccgcgc
     4081 tcgcgccggc ccactcggcg tgcacccggc cggcgtcggc gcggaaggcg gcgccggcgg
     4141 cggccccgag gcggtgcgga tcggcatacc cctgctcgtg cgcgagcgtg gagatcgcgc
     4201 ctccgggcga cagtgcgatg ccgagcgtgg accacggcgc cgtgccgtcg tgggggcggt
     4261 tgatgccggc gagcacgatc gcggcgcggc cggatgcgcg ttcgctgatc cgccagaagt
     4321 agccctccat cgccagcccg tgcgcgctga gcgggtcccc gaagggcagg tcggcgccgc
     4381 tggcccggta tgccgcgtgg agcctcctga gggccgtcac ccgcgccacg cctcggagaa
     4441 gtcggcgtgc tcgcgcaccc aggcatgcat cgcgaccgcg gcggcgaccg aggcgttgat
     4501 ggatcgggtg gatccgaact gggcgatgga aaatgttgcg gcgcaggcat ttcgggcctg
     4561 ctccgagagg ccggggcctt cttggccgaa gaggaagcac acgcgtcggg gcaggggtgt
     4621 atcttcgagg tggagggagc cgggcaggtt gtcgacgccc cacagtcgca cgtcccgctc
     4681 gtgcaggtag ccggcgagtt cggtggcgtc ggcgtgatag cgcaggtgtt ggtagcggtc
     4741 ggtgaccatg gcgccgcggc ggttccagcg gcggttgccg acgatgtgca cctccttggc
     4801 gaggaaggcg ttggcggtgc ggacgatggt gccgatgttg aagtcgtgct gccagttctc
     4861 gatcgcgacg tggaagtcgt gccggcggct gtcgaggtcg gcgacgatcg ccgccatcga
     4921 ccagtagcgg tacttgtcga cgacattgcg ccggtcaccc tcacgcagca gtgactcgtc
     4981 atagacctcc gcgcccggcc cgttcggcca ctcgcccacc cacggcccca ggccgacctc
     5041 ttgggggccg tggggcatgg ggtcgtaagg ggcgcgctgc tcctgcgtca ctggagcgcc
     5101 gagagatcgt cgggtgcgtc cgcggcatac ggcagcagca cctcgagggg gatgacttcg
     5161 agcgtcgcct cgtgggtggc ggccaggatg accggcgcgg cggcgccgtc ctggtgggcg
     5221 tgcaaccagc gcagggccac gacgcaccag gtgtcgccgg gctgcaggcc ggggaagcgg
     5281 tatgccggga tgggcgtgat gaggtcgttg ccggtgcgct gctggtggtc gaggaactcc
     5341 ggcgtcatga ccgcacagat ggtgtgccgg ccgaagtcct cgggcgaggt ccagcacgcc
     5401 ccgttgcgga agaacccggt catcgggtcg gtgccgcact cggcgagggg gccgccgagc
     5461 acattgcgag ccgtcgtcac gctgggcaga ttacctgccg ggcgtcctcg cggagtcgag
     5521 cggccgcggc gcagcacgga cgtcgagcca catggcgccg gcgacgagcg cgaggccacg
     5581 tgcgagccac gccctggacg gcaggtcgaa cggggttatc ccagcggtgc gggatccttc
     5641 gggtttgggc gcagcaggcg ggcgagccgg tatgccggtg tccgcagcgt cgtgaccaac
     5701 ggatagctgc ctgccccggg agtggggtgg gcgacggcgt cgaagcggat ccgctcgtcg
     5761 tggtcgctcc aggcgacccc gagccgcccg atcggctgcc acccgccccg gcccagggcg
     5821 gcatacagct gatgctccgc aggcgggaga agtccctccc cgtggccctc aggttcggcg
     5881 cgcagcagca gcgggccggc gggggagtcc accgggagga gggtggtcag cgtgccgaag
     5941 gtgccgggct cgcgggtctg cagcacgaag cggtccagcc ctccggcgcc cgtggaggcc
     6001 aagagccagt cgacgagcgc cccctcggcc tcgaagcgca cggccagccc gtggatgtcg
     6061 gcgcgctccg gaccgacgcc gatcgcccac gagaagcgag cggtcacctc atacgatccg
     6121 ggggtgtcga ggacgggcac tcggagcgcg gccgcgcccg agatgtccag cactccccgt
     6181 ccgacgacgc ccttcgggtg gaggggtttg cgatcgcgca gcagcgcgag tgccgaggtg
     6241 gccccggcaa tcgccacgcc ggggaccaga ccgagaagtc gttccacagg tgagggcagc
     6301 accgggcgag cataggccga tccccgctcg ggaacagcgt tgtcggtggg ggcgtttacg
     6361 gttgaatcat gcgtccggtc actgaactga agcggcgggt ggcccccttc gaggtcgtct
     6421 ccgaattcga gccgagcggc gatcagccgc aggccatcgg cgacctcgcc acgcgggtca
     6481 aggcggggga gcagaacgtc gtcctcatgg gcgccaccgg caccggcaag tcggcgacga
     6541 cggcctggct catcgagcag gtgcaacggc ccacgctggt gatggcgccc aacaagacct
     6601 tggccgccca gttggccaac gagttccgag agctgttgcc gcacaacgcc gtcgaatact
     6661 tcgtctccta ttacgactac taccagcccg aggcctacgt cccgcagacc gacacctata
     6721 tcgagaagga ctcctcgatc aacgacgagg tcgagcggct gcggcacagc gccaccaact
     6781 cgctgctgac ccgccgcgac gtcgtcgtcg tggcgtcggt gtcgtgcatc tacggcctgg
     6841 gcaccccgca ggagtatgtc gaccggatgg tgccgctcaa ggtcggcgac gtcatcgagc
     6901 gcgaccagct cctgctgaag ttcgtgcaga tgcagtacac gcgcaacgac ctggccttca
     6961 cgcgcgggac cttccgggtg cgcggggaca cggtggagat catcccggtc tacgaggagc
     7021 tcgcgatccg gatcgagttc ttcggggacg agatcgagcg gatctacacc ttgcatccgt
     7081 tgaccggaga ggtggtgcgc gaggaggagg agatgtacgt cttccccgcc acccactatg
     7141 tcgccggccc ggagcggatg gagcgggcga tctccggcat cgagaaggaa ctggagcatc
     7201 agctcgcgac cttcgagaag cagggcaagc tgttggaggc ccagcgactg cggatgcgca
     7261 ccacctacga catcgagatg atgcgccaga tcggctcgtg cagcggcatc gagaactact
     7321 cgatgcatat cgacggccgc tcgcccgggt cggcgccgaa ctgcctgctc gactacttcc
     7381 ccgacgactt ccttctcgtc atcgacgagt cgcaccagac ggtcccgcag atcggcgcca
     7441 tgtacgaagg cgacatgtcc cgcaagcgca ccctcgtcga gcacggtttc cggttgccca
     7501 gcgccatgga caaccggccg ctgatgtggg aggagttcct cgaccggatc ggccagaccg
     7561 tctatctctc ggcgaccccg ggcaactatg agctggcgaa ggccgacggc gtcgtggagc
     7621 aggtcattcg ccccaccgga ctcgtcgatc ccgaggtcat cctcaaaccg acgcgcgggc
     7681 agatcgacga cctgctcgac cagatcaatc agcgggcggc ccgcaacgag cgggtgctgg
     7741 tcacgacact gaccaagaag atggccgagg acctgactga ttacttgctc gagaagggcg
     7801 tgcgggtgcg ttatctgcac tccgacatcg acacgctgcg ccgggtggag ttgctccgag
     7861 aactgcggtt gggcgagttc gacgtgctcg tcggcatcaa cctgctccgc gagggtcttg
     7921 acctgcccga ggtgtcgctc gtctcgatcc tcgatgccga caaggaaggc ttcctgcgct
     7981 cggcccggtc gctgatccag accatcggcc gcgcggcgcg caatgtgtcc ggtcaggtgc
     8041 acatgtatgc cgatcagctc accccgtcga tgcaggaggc gatcgaggag accaaccgcc
     8101 gccgcgagaa gcaggtggcc tacaacctcg agcacgggat cgacccgcag ccactgcgca
     8161 agaagatcgc cgacatcacg gacatgctcg gccgcgagga cgccgacacc gacaccctgc
     8221 tcggatccgg gcgcacccag tcgcgcggca cccgcggcgc cggccgcggt ggccgcgggg
     8281 cgatgatggc cgacaccggt caggtcgcga cgatcaaggg cctgcccgcc tcggatctcg
     8341 ccaacctcat ccaggaactc accgagcaga tgcaccaggc ggccgcggag ttgaagttcg
     8401 aggtggccgc tcggttgcgg gacgaggtca aggacctgaa gaaggagttg cgccagatgt
     8461 ccgaggccac tcgctgacgc tcgcccgacg cgcgtccctc tgccacactc gccccatgac
     8521 gcccgaagag gttcgtgacc acgccttggg caaacccggg gcacaccccg acgagccatg
     8581 ggagggcgac cacgtcgcca aggtcgtcga gaagatcttc gtcttcggtc tcggaggcga
     8641 ctcggtcggg gtcaagtgcg gagccaaccg agccgaggcg gatgagtggc tccaccagta
     8701 ccccgacgat gcgacggtca tggcctacat cgggcgtaac gggtggaaca ctctgcggat
     8761 cggtggggcg atccctgacg gggagatcct ggaggccatc gacacctcat acgatctgat
     8821 cgtcagcaag ctgccgaagt ccaagcgccc cagctgaatc cggcatcagc gtcgcgacgt
     8881 gaagcgcagg gagtacccgt ccgggtcaac gacccggaag tcggtgagcc cccagggccg
     8941 ctcaacgaga tcctcatcga gcacgagtcc ggcggcgccg gtcggccatg ctcaacgtcc
     9001 tcgccgtggt cgtcgagctc ggcatcgatg cacacctatg cccgatcagt gatcccacat
     9061 gtccgagatc gtcgtcaacg tcatcccccg ggaccgcaag gtgggcacga tcgtcttgag
     9121 cgcctcgccg gtgtgatagc cgccgaggtg cgacaggacg atggttccgg tctcgggggt
     9181 cgggtcgagg acggcgtcga tgatgtcctg ggtcgtggtc gcgggatccc agtcgatcgt
     9241 gtcgcgactc cacatcatcg tcttcggata accgacgcct gcgacgacag actgaacgtg
     9301 gctgtcatac acaccgaacg gtggacgcca gtagggccgg atcggcatgc cagacaggcg
     9361 cttgagcacc gattcggcgt cgctgagttc ggaccggatg aagctgtcgg tcatcgaccg
     9421 ctgacacgga gctgacgtgg gcgacccgcc gccgccgttg acgagatcgc agtggtgcac
     9481 cgtgtgattg ccgacctcga acagctccgg gtgcgcggcg atgagtccga ggatctcccg
     9541 gccttcgctc gtgtcggcca tgatcgaggt cgggaagaac gtggtgcaga cctggttgtc
     9601 gatcaggtag cggacgatgt ccgcgccgcc gtcgagccgc ccgcccatgt cgagggtgta
     9661 tgcgatctgc tgcacggtca tcccgtagat cttcttgacc gcctcgacgt cggtgttcac
     9721 cggtggtttg ccggcccagc acaccccgag ttccttctgg accagccgcg gcacattgat
     9781 gggcaccgac gccggaccgc cgaggatgta tgccgtgtcc ggtgacaacc gggtgagctc
     9841 gtcgctcgtg ccgctcggca gggtgtctcg ccgcgagagc aggatcggcc cgcgggtcct
     9901 tgtcgcctgt gcgctgccgg ccaacgcgtc ggggtagttg gcgccggtgg cgatcatcat
     9961 ggcgggccgg tcggtgccga agatccgctt ggatatggcc agtgcggtgt cgtaacggtc
    10021 atcgccccaa agacgttcga cgggagcgat atcggcgagg tctgcggcga cgcccgcgtc
    10081 gacggatgcc gtgccaccga gcaggaagat ccgcttgggt gccagccgcc gcagttcggt
    10141 cgcgatcacg gccggcaccc ggtcgggctc ggtgaggagc atgggtgagc cctgcaccgc
    10201 ggcggcggcg ccgccggtca aggcgtccgg ccagacccgg ccggaggcga cgtatgccgt
    10261 ctcgaccgtc cccgtgaaaa cttcggcact cgccttggcg gccaccgcga agcggtccgc
    10321 cccgtcgagg cgttcggccc cttcgtcggt ccaggcgctc aggtcgacca tgacctcgtc
    10381 gctgatggtg gccctaccgc ccaggaccag gatctggctc ggtcgcagcc ggtcgagttc
    10441 ctgcgccgtc gccttaggca cgctgtactt cgtgacgaac aggaccggag caccgagctt
    10501 ggccgccacc gggccggcgg cgacgccgtc gctccacagc tcgccactgg cgatgaccac
    10561 ccagtgtgac gtgccgggct ggaactcacg gctgatcgcg gccgccgcgg cataccgatc
    10621 ggcgccggcg agccgctcga tgcggaagga caagcttcgc gcgccggcct gcgacgagac
    10681 gctgaccccc gtcgccggcg ccgacgccat cggagccacc ggagccacgg ccgaggaacc
    10741 accacacccg agcagcgcaa gcgccgcact gagcacgcag gccgacagga ctgacatacg
    10801 gaaccggcgc atggcgccct ccttcactcg gacttccaca gtgttcacgc ctggaccgac
    10861 aacccggttg accgggggag caagatgggt ggcccccgag ccgacgcttg cggcctaact
    10921 cgatgcggga cgctagactc tgcgaacgcg aagggtgggc ctccgagcgg acgcttgcgg
    10981 cctaattcaa tgcgggacgc tagactctgc gaacgcgaag ggtgggcccc gtgagcgggc
    11041 ggcggagtct gctcgccgga ggcgctagac tctgcgaacg cgaagggtgg gccccgtgag
    11101 cgggcggcgg agtctgctcg ccggaggcgc tagactctgc gaacgcgaag gggagtatcc
    11161 cgacacgtca gcctcgtcat cacggtccca ccggacccgg ggcggcggtc cactcgtggg
    11221 cgggagagac cttcggactg aatcacgtca tgtccggaag gggccctgct ttgaccacga
    11281 tgcttgctgc ggcgaatgag acgcaactga acttaccgac ctgggcgtgg tggctcacga
    11341 tcgggattgc gatcgccgtg ttggcgttcg acgtcctgtg gatcatgcgc aatccgcacc
    11401 gcccgtcgac caaggaactc gtcgtcgccc tcagtatcta catcggtgcg gccgtggcct
    11461 tcgggatcgc cctgctcgtc accaaaggtg gcaaactgtc gggcgagttc ttcgccggct
    11521 ggctgaccga gtacagcctc agcgtcgaca acctcttcat cttcctgctg atcatggcga
    11581 acttcgctgt gccggaacga cttcagcagt tcgctctgat gatcggcatc gtcatcgcga
    11641 tcatcctccg tggcctgttc atctgggccg gcgcggcggc gatcaacaac ttctcctggg
    11701 tgttctacat cttcggtgct ttcctgatct acaccgcgtg gaaactggcg agcggcggcg
    11761 aatcggatga tgacgaatac gaagagaaca agttcatgaa gttcgtcgag aagcgcattc
    11821 cctcgacgaa ggagtaccac gacacgaagc tgttctcccg ggtcaacgcc cgccgggtcg
    11881 ccacgcccat gctgttcgtc atcctcgcgc tcggcacgac tgacctgctc ttcgcgctcg
    11941 actccatccc cgcgatctac ggcctgacca aggagcccta tatcgtcctg gtggccaacc
    12001 tgttcgcgct gatgggtctg cgccagctgt acttcctgct cggtggcctg ctccagaaac
    12061 tcgtctacct gagcatcggc ctggccgtcc tgctcgcctt catcggtgtc aagctgctgc
    12121 tgcacgccct ccacgagaac gagctgccgt tcatcaacgg cggcgagcac gtcagcgtgc
    12181 ccgagatccc gatcgccgtc tcgctcggcg cgatcgtggt catcctcggc gtcaccacca
    12241 tcgccagcct ctggaagtcg aagcgggacg cgcgcctgga ggcctgaagg caggcggcct
    12301 agaagccggt atgcggtgcg gccggggccg cgcgtccact caggtggccg cggcctcggc
    12361 cggatccttt tcggtgcctc cgccgcccat gatcaggatg ctcgcgccca cgacgagcgc
    12421 catgcccagc aactgccaga cgtgcagccg ttggccaagc acgagcaacc ccgccagcgc
    12481 cgcgcacgcc ggctccaggc tgagcaggat cccgaagacc gcggcgctca tgcggcgcaa
    12541 cgcgatcagc tcgagcgaat acggcaggag cgaggagagc acggcgatgc cgagcccctt
    12601 cgcgagcagc ggcgcgctcc agtcgccgac ggtggccagc ccgaagggcg cgacgaagca
    12661 ggtcgcgatg agcatcgcga tcgcgagccc gtcgagcttg gggaaggcgg ccccggtcct
    12721 cccgctgagg atgatgtatg ccgcccagca cgcccccgcg gtcagcgcca gcaggatccc
    12781 ggcccagctg agctgatcca agggcacgtg cagggcctcg gatatcagga ccaccccgac
    12841 cgcggcgccg gcgaccgcca catagtccga ggggcggcgc gagaggactg cgctcagcag
    12901 cagcggcccg atgaactcga tcgtcaccgc caccccgatc ggcagatgcg ccagcgagcc
    12961 gtagaaggcg aagttcatca gcccgagcgc gagcccgaac aggatcaccg tgcgccaggc
    13021 tgccgcgcta tacccccgca accggggccg caccaccgcg cagaggatga cgctcgcgaa
    13081 gagcagccgc atggtgaccg acccggccgc cccgatgtgc gggatcaagg tcgccgcaag
    13141 cgccccgccg aattgcactg agacgatgcc cgcgagcacc aataacggcg cggggacacg
    13201 gtcggcagca gtcacccgag cagggtaggc gttcgccagt cccggtggtg gtcaggtgag
    13261 gtagcgacgt catagcgtcg ctccctcgca cacgaccaag gccattgctc gactgagcac
    13321 cccgccttga ggaggtcgtc atcacccgtg ccgggcacga ccccgtcgtc atcgtctccc
    13381 ttgccgacta cgagtcgctt cgggagacgg cttatctcat gcggtcaccc gccaacgccc
    13441 ggcgcttgct cgatgccatg gagcgcctcg aagctgggca gggccaccct catgacctca
    13501 tcgagaccga ctgaacctgt tgctggtctg ggacgagaac gcctgggaag actaccgctg
    13561 gtggcagcgg caggaccgtc aggtccgcaa gcgcatcaac ctcctgctga ccgacatcac
    13621 ccgcaacggc aacgagggaa tcggcaagcc ggaggcgctc aaacacgact tcgccggctg
    13681 ctggtcacgc cgcatcacgg acgagcatcg gctggtctac aagatcgacg gtgaccaggt
    13741 gcgcatcgcc gcatgcaggt accactacgg gtagccacgg tcgtcggccg tgctatgggt
    13801 caccagccgc gggcgcgcca ctcctcgagg tgcgggcgtt cggcaccgat ggtcgagtcg
    13861 ttgccgtggc cgggatagaa ccacgtgtcg tcgggataga catcgaagat gcgctcggtg
    13921 acatcggcat acagcgactc gaacgactgg ccgggcagct tggtgttgcc cacgccgccg
    13981 gggaagagcg agtccccggt gaagaggtgg gtgatgccgc cggggtcggc atacgcgagc
    14041 gccacgccgc cgggggtatg cccgcgcagg tggatcacgt cgagcgtcac ctcacccacg
    14101 gtgatcgtgt cgccctgcct caggcgcacg ccgggcgcca ccgggagcgc gtcggcgtcc
    14161 tcctcgccgg cgtaggtcgt ggctgctgtg gctcgggcga cgtcctcgag ggcgcgcacg
    14221 tggtcccagt gctggtgcgt ggtgacgagg tgcgatgccg cgccggtgcc ctccgcgatg
    14281 aacgacatga tccggtcggc gtcgtcggcc gcgtcgatca acagctgctc gcccgtggcc
    14341 cggcaggtga tcagatagac gttgttgtgc atgggggaga cggacatctt ccgtatggtg
    14401 gcccggtcca gctcgcgcac atcgctcggt ccgcccgggt ccacgacgcc cgtgtagtcg
    14461 gtcgatcccg gagtcatcgt gggtccatcc cgcgggcgag ccgggtcaac gcggtgccga
    14521 gagcggccgg atcgctcgtg tcgaccatgc cgatctcctg ttcccagagg cgtcgcagca
    14581 acgccggctc gacgtccgcg aaaccgaagc cggcatcgag atcgacgtgg tgatagatca
    14641 actcgcgcaa ccgcataccc ggcaggttgc ccgcctggaa ggtgggccca cccggcaccc
    14701 gctcgacctc gcgtgcgggg tcggcagcgg ccagccgagg cagtgccgcg accagcgccg
    14761 cgccctggtc gcgcgcgaac gcacgcaact catccggcgg caactgggcc gaccggtcga
    14821 tgtcggcgtc ccggtcggca acgctggcgt acatcgtctc gccactgtcg tcacacgcgg
    14881 cccggaccag ccgcagcagg ccttcggcgt tgtgcccgag gtgagcgatg atgtgggctc
    14941 gtgtccagcc ggtgcacagg ctcggctggg tcaggtccag gtcggcggcg gcgtcgtcca
    15001 cggcggcggt atgccgcgcc acagcggcca gcaccgtatc gatcggcgaa gtctcgttca
    15061 cgtgccgacc ctagcgagcg caggcgggcc gggccaggct cagcccgcct tgcgggcccg
    15121 gcgcgccgca gcagatgcgg ccttcgacac ctcgaccttg ggcggcacga ccctcttggc
    15181 cgcgaccttc ttggccgggg cgcgctgtgc cgttgccttc tgtgccgttg ccttctgtgc
    15241 cgttgccttg gttgccgtgg ccttggttgc cgttgtcttg gttgcggtcg tcttcttcgc
    15301 cgtggcctgc accgtcgcgg aggctcggtg cggggtccgg gcggaacgct ccagaatcgg
    15361 cgccaggaag cgcccggtgt gcgaatcctg atctgccgca acatcttcgg gagtgccttc
    15421 ggcgatcacg cggccaccgc cggacccacc ctcgggtccc atgtcgatga tccagtcggc
    15481 gttcttgatg acgtcgaggt tgtgctcgat gacgatcacc gtgttgccct tgtcgaccag
    15541 tccctgcagg acgatcagca gcttgcgaat gtcctcgaag tgcaggccgg tcgtcggctc
    15601 gtcgagcaca tagatcgtcc ggccggtcga gcgcttctgc agctcggagg ccagtttgac
    15661 gcgctgcgcc tcgccaccgg atagcgtcgt cgccggctgg cccagccgga cataccccag
    15721 accgaccgca ttgagcgtcg acatgtggcg cgcgatcgcc ggcaccgccg cgaagaactc
    15781 ctccgcctcc tcgatcggca tgttgagcac gtcggcgatc gtcttgccct tgaagtgcac
    15841 ctcgagggtc tcgcggttgt aacgcgcccc atgacacacc tcgcacggca catagacatc
    15901 cggcaggaag ttcatctcga tcttgatcgt gccgtcaccc gagcaggcct cgcagcgccc
    15961 gcccttgaca ttgaaggaga accggccctg ctggtagccg cggatcttcg cctccgtggt
    16021 cgtggcgaag agcttgcgga tgtggtcgaa cacgcccgta taggtcgcag gattcgaccg
    16081 cggcgtccgc ccgatggggc tctggtcgac gtgcacgacc ttgtcgaggt gctccaaccc
    16141 cgtgaccgac ttgtgccggc cggggacgtg ccgggcgccg ttgagcttgt tggccagcac
    16201 gttgtagagg atgtcgttga ccagcgtcga cttgcccgac ccggacacgc ccgtcacggc
    16261 tacgaaggtg ccgagcggga aggacacggt gacgtcgtcg aggttgtgct ccttggcacc
    16321 gacgaccttg accatgcgct gccggtcgat gggccgccgc accgggggag cggggatctc
    16381 gagccgcccc gaaagatatt gccccgtaat ggaatcgggg tggttgagca ggtcggtcac
    16441 cggcccgcag tgcacgacgt ggccaccgtg ttcaccggcg cccggcccga tgtcgacgac
    16501 ccagtcggcg tgctcgatgg tgtcctcgtc gtgctcgacg acgatgaggg tgttgccgag
    16561 gtcgcgcagc cgc
//
LOCUS       DAOPJG010000060        16197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3794_length_16197_cov_7.418040, whole genome shotgun sequence.
ACCESSION   DAOPJG010000060 DAOPJG010000000
VERSION     DAOPJG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3794_length_16197_cov_7.418040"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKU75_07460"
     CDS             <1..589
                     /locus_tag="PKU75_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPF80750.1"
                     /translation="DRGLASSRVLAGTIRRTGDDAHDLALSASLARSSKDLEEHEYAV
                     RSVADALGRHCASMNVPESPFVLHLPNVMHLATDVAGAVEDGATSLELAASLHPSAAV
                     CGTPTATADALITELEGFDRGRYAGPVGWMDAAGDGEWGIALRSGQLDPADPCQISIF
                     AGCGIVAGSDPEDEVAESDAKFLPMRDALTAAPTA"
     gene            complement(676..1692)
                     /locus_tag="PKU75_07465"
     CDS             complement(676..1692)
                     /locus_tag="PKU75_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80751.1"
                     /translation="MRPIELPEELAGVAFTSDRARALGVPSKALRTSRLEHPTRGVWA
                     QHPVGDDLRERARAAHLAMCGDAAFSHVTGARLLVLPLPPRALQQRRLDVCTRTDVGQ
                     RRRDGWVGRRGLETRQVVVVDGLRVVDLVDTWIDLAALTVGRHPTMTRVDLVVVGDEV
                     LNRLLCLANAAQLTPRRKPYLDAALVAAAYREIDAAIARRIRPRGKRNLLWARERIRP
                     GVRSPKETEARLVVVEAGLPEPLINHDITAPDGSGWLGEGDLVWRTRDGWVVVEYQGA
                     HHADRRQASADELRQRILEDNRCAVFPMWAEDLDPGARQRAFLARVAAALTRQCGPTP
                     PQVE"
     gene            1945..3237
                     /locus_tag="PKU75_07470"
     CDS             1945..3237
                     /locus_tag="PKU75_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HPF80752.1"
                     /translation="MSETAEKADVIVVGAGPAGSSTAAYLAMSGLDVLLLEKTSFPRE
                     KVCGDGLTPRAVKELITLGIPTPEEEGWIRNKGLRIIGGGMRLQLDWPDLASFPPYGL
                     VRTRQDFDDILAKHAVKHGARLRESTNVTGPIRDESGRITGVTAKASDEKGRAVGPEQ
                     RFEAPLVIAADGNSSRLSMAMGREKRDDRPMGVAVRTYYTSPRHDDDYLESWLELWSS
                     DDEGNKILLPGYGWIFGVGDGTSNVGLGILNTSSAFGKTDYKDVMRRWVATMPKEWTY
                     SDETMVGPIRGAALPMGFNRQPHYRDGLLLVGDAGGMVNPFNGEGIAYAMESGRLASE
                     VIAQAFARGDDAGRERVLASYPAVMKDDLGGYYTLGRWFASAIGHPEVMRLATKYGLP
                     RTTLMKLLLKIMANLAEPRGGSASDRLIVALSKMAPDA"
     gene            3335..3697
                     /locus_tag="PKU75_07475"
     CDS             3335..3697
                     /locus_tag="PKU75_07475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPF80753.1"
                     /translation="MHNPYVPLLFFLAIGAGFAIFSVGISPITGPRRYNRAKMDAYEC
                     GIQPSPQAQGGGRVPIKYFLTAMLFIIFDVESVFLYPFAVAFGGLGLFALVEMVLFIL
                     TVFIAYAYVWRRGGLDWD"
     gene            3714..4295
                     /locus_tag="PKU75_07480"
     CDS             3714..4295
                     /locus_tag="PKU75_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPF80754.1"
                     /translation="MGIEEKLPAGFLLSTVENVAGLLQKSSIWPATFGLACCAIEMMA
                     VGTPDYDIARFGMEKFSASPRQADLMIVAGRVSQKMAPVLRQVYDQMPNPKWVISMGV
                     CASSGGMFNNYAIVQGVDHIVPVDVYLPGCPPRPEMLLNAILTLHDQIRHSKLGVNRV
                     AAARAAEKAALEATPTSLMAPTHDHLAGKSLLA"
     gene            4292..5086
                     /locus_tag="PKU75_07485"
     CDS             4292..5086
                     /locus_tag="PKU75_07485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPF80755.1"
                     /translation="MTETPKPADQPPAEPAKPNEPAIDRAEKTIETPPVPGAENLPAQ
                     PGTTPEPVQRGERTGMFGAHDTGDTTGYGGLRSPILYPGAAVRPYGGDFDAIADALEA
                     ATDQGQIARTVIDRGEITFVIPREHLPAVVRALRDDPALRFELCLSVSGVHFPSDIGN
                     ELHVVYHLMSITNGHRRIRLEVTAPDADPHIPSVVGTYPAADWHERETWDMFGVVFDG
                     HPALTRILMPDDWPGHPQRKDYPLGGIPVEYKGGTVPPPDQRRSYS"
     gene            5086..6501
                     /locus_tag="PKU75_07490"
     CDS             5086..6501
                     /locus_tag="PKU75_07490"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPF80756.1"
                     /translation="MTTPHSESGSTVYTAADPDEVRHIDDLDEQADLYAAPGEPSEGR
                     VFNAVGGDWNDIVGEVGSRPEERIVVNMGPQHPSTHGVLRLILEIEGETVTEARAGIG
                     YLHTGIEKNMEFRTWTQGVTFCTRMDYLMPIFTETAYCLAVEKLLGITDQIPARASAI
                     RVLMMELNRIGSHLVCLGTGGMELGATTVMTIGFRERERILNIFEMVSGLRMNNAYVR
                     PGGVAQDLPAGAIQAVRDIIPELRQGIRELELLMLENPILKGRLVGIGYLDLTGCMAL
                     GITGPMLRSAGYPLDLRKATPYCGYEKYEFDVPTRTGADAYDRLWLRVKEMWESLKIV
                     EQVVAELQDNPGPVMVEDKKIAWPAQLSVGPDGMGNSPAHIAEIMGTSMESLIHHFKL
                     VTEGFRVPPGQAYAAVESPKGELGCHAVSDGGTRPYRAHFREPSFNNLQAVAALCEGA
                     QLADVIVAVASIDPVMGGVDR"
     gene            6501..7358
                     /gene="nuoE"
                     /locus_tag="PKU75_07495"
     CDS             6501..7358
                     /gene="nuoE"
                     /locus_tag="PKU75_07495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005721.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPF80757.1"
                     /translation="MEHYGEQTASGFLHVSAESKEPYAADVLAKLHQDAEAVIARYPQ
                     KRSALLPLLHLVQSVDGYVAGRGVRFCSEVLGLSEAEVSGVATFYTQYKRHPNGDYTV
                     GVCTNTLCAIMGGDKIWDTVSEHLDLEHDETSSDGKITLERVECNAACDFAPVVMVNW
                     EFYDNQTPESTVQLVDDLRSGAPVKPTRGPDRQCTFKEMSRVLAGFHDGLADQGVGAG
                     SASLAGLRVAREQGWVAPGDAGSDSGSGPVADVAPVADSALQGERDKMTDVAGESLQR
                     AQQTEQAGE"
     gene            7363..8673
                     /gene="nuoF"
                     /locus_tag="PKU75_07500"
     CDS             7363..8673
                     /gene="nuoF"
                     /locus_tag="PKU75_07500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPF80758.1"
                     /translation="MTQLTPIFTRYWDQPNSWTLETYAKHSGYEGLKKALGMPPADII
                     ASVKDSGLRGRGGAGFATGVKWGFLPPPDGGPRYLVVNADESEPGTCKDIPLMMATPH
                     FLIEGVAITSFAIGCNHAFIYLRGEVVHVYRRLLRAVEEAYAAGHLGKNIHGSGFDLD
                     VTVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYARPTVVNNVESIASVA
                     PILLYGDEWFSAMGTEKSKGYGFFSLSGHVKNPGQYEAPLGITLRELIDMAGGMRDEN
                     HPLKFWTPGGSSTPLFTQEHLDVPLDFDSVAAAGSMLGTRALQIFDDTTCVVRAVDRW
                     TDFYKHESCGKCTPCREGTWWLKQILGRLEHGHGDEEDLEKLLDVCDNILGRSFCALG
                     DGATSPVTSSIQYFRDEYLAHLTQGGCPFDRDASAVFAKENAHA"
     gene            8670..11105
                     /locus_tag="PKU75_07505"
     CDS             8670..11105
                     /locus_tag="PKU75_07505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HPF80759.1"
                     /translation="MSVTPAQTDLVNLTIDGIPVSVPKGTLIIRAAEQIGIQIPRFCD
                     HPSLDPVGACRQCLVEVAMPGPPGPDGKPSAPRQMQGPPGRMKPQASCTMTVAEGMEV
                     KTQYTSEGADKSQQGVMELLLINHPLDCPVCDKGGECPLQNQAMSSGRAVSRFEDVKR
                     TYPKPINISSQVLLDRERCVLCARCTRFSDQIAGDPFIALVERGALQQVGIYEEQPFE
                     SYFSGNTVQICPVGALTGAAYRFRSRPFDLVSTAGACEHCASGCAIRTDHRRGVVLRR
                     MAINDPEVNEEWNCDKGRWAFTAPTVGDRISLPMVRGASGQLQVASWPEALAAAAAGL
                     QGQSAGVLTGGRISVEDAYAYGKFARTVLATNSIDFRARPASADEARFLAASVVATGP
                     AGGSVTYAALEKAPKVLLVGFEPEEESPIVFLRLRKAFRKNKTQISSIAPFATRGLGK
                     MGGTWIPAAPGTETEILGSVDAAAALDGEGSVLLVGERLATVPGALAAATALAERTGA
                     TLAWVPRRAGERGALEAGALYNLLPGGRPAGDAGARADLAATWQVDALPGDTGLDGAG
                     IIAAAASGELGALVVGGVDASDLGDGAAYDALERAFVVSLEYRPSSVTAAADVVLPVA
                     PHAEKAGTFVDWEGRVRPFAAALETNALSDYRVLDMLASELGHFLGARSLEQVQTEMS
                     TLGPWSGARASKPAALPAQVPSTRPGQFVLATWHHLLDKGRLQDGEPFLAGTAPAAVA
                     RISASGAQALGVHDGEQVTVTGPAGSVTVPLAVTEMVDNVVWVPTNSAGCRVAVLGVG
                     AGSLVTVTPGGAS"
     gene            11111..12454
                     /gene="nuoH"
                     /locus_tag="PKU75_07510"
     CDS             11111..12454
                     /gene="nuoH"
                     /locus_tag="PKU75_07510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPF80760.1"
                     /translation="MTVLAAAEPYVPRDNPVADFSDTPVWLSLVKALFLFVYLLLSTL
                     LVIWFERRVIGRMQQRPGPNRFGPFGIFQTLADGVKLALKEDIVPKNADKLIHLLAPI
                     LAASMAFVSFAIIPLAGNVRMFGHTTPLQLTDVPIAVLIALAVAGVGAYGVVLAGWSS
                     GSTYPLLGGLRSSAQIISYEIAMGLSLVAVFLYSGSMSTSQIVANQRSIWHVIPLVVS
                     FVIYVITMVGETNRLPFDLAEGEGELTAGFMTEYSSMKFAMFFLGEYINMFTVAGLAT
                     TVFLGGWQAPPGIAAINDGMFNHGWWGLLWFTVKLWLFMFLFVWLRGSLPRTRYDQFM
                     RFGWKFLIPVTIVWVVAVAFLQAADHGYLGEKVVSIFGAERRLASLIVIGALALLVLA
                     ATWIWEGRQAAKIEAAKPVVPDAIDPYAGGFPVPPLPGQRLEQAAPAPTKEVLHG"
     gene            12447..12983
                     /gene="nuoI"
                     /locus_tag="PKU75_07515"
                     /pseudo
     CDS             12447..12983
                     /gene="nuoI"
                     /locus_tag="PKU75_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
     gene            12995..13762
                     /locus_tag="PKU75_07520"
     CDS             12995..13762
                     /locus_tag="PKU75_07520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPF80761.1"
                     /translation="MTSTAEAVTFWILGSIAVLMSLGLLFARKAVYAAMGVAVTMTIL
                     GIFYVAQGAPFLGVVQVFVYSGAVMMLFLFVVMLVGVDSSDSLIETIKGQRWIGLLLS
                     LGLAMLLAGMIGKVTIDGTPTLDAVNQGGHISAMANVIFGKYVWVFEITSALLITAAL
                     GAMVLAHRERLGAKPNQKAWSQERFRRGEYVSGLPAPGVYARHNAVDTPALLPDGTPT
                     ELSVSRTLQARDQVALPGQYTAAEQQLQKEIEEGRTR"
     gene            13759..14058
                     /gene="nuoK"
                     /locus_tag="PKU75_07525"
     CDS             13759..14058
                     /gene="nuoK"
                     /locus_tag="PKU75_07525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPF80762.1"
                     /translation="MSLMNYIYLAMILFAIGAATVLTRRNAIIVFMGIELMLNSANLA
                     FVTFGRMHQSIDGAAIALFVMVVAAAEVVVGLAIIMAIFRARRSASVDDANLLKY"
     gene            14136..16052
                     /gene="nuoL"
                     /locus_tag="PKU75_07530"
     CDS             14136..16052
                     /gene="nuoL"
                     /locus_tag="PKU75_07530"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPF80763.1"
                     /translation="MPGWWLIALPLAGAALLLLGGRATNSFGPALATGLSWASFGLGV
                     VALLQLLAVDPDQRSTHQTLFNWIPAGAFQLSAGLQIDTLSVSFVLLITFVGSLIHVY
                     SLGYMSHDPDKRRFFAYLNFFVAAMLLLVLADSFLLLFVGWEGVGLASYLLIGFWNTR
                     EAGAIAPGHFAEANAAAANKAFVVNRVGDIGLSLAIALMFATFGAVDFAAVNAGVAAG
                     GAGTATLTAIGLLLLLGACGKSAQFPLQSWLGDAMAGPTPVSALIHAATMVTAGVYLV
                     VRSHTIFEAAPNAQLAVAIVGAITLTYGAIVGCAKDDIKKALAASTMSQIGYMMLAAG
                     LGPVGYAFAIFHLITHGFFKAGMFLGAGSVMHGMSDQVDMRRFGNLNKFMKITWITFG
                     LGWLAILGVPPFSGFWSKDKIIEAAFIGEGWRPWVFGLAALLGAGVTAFYMSRLFFMT
                     FHGKERWTDEPKQHPHESPLTMTLPMMVLALGSAGLGLILGPTDIFTKWLEPVTGHPE
                     HGEPVLSVPVLMTLTLLLVALGAGLAWLRYGRDEVPMYAPAGNALTQAARKDLYQDDI
                     NEAVFMWPGVKLVRSVIWTDNEVVDGGAMGLGKLTGGTATWLRRIQNGYARSYALTML
                     AGIVAVLGALWVMN"
BASE COUNT         2480 a         6013 c         5140 g         2564 t
ORIGIN      
        1 cgaccgcggc ctcgcctcct cccgcgtcct ggccggcacc atccgccgca ccggcgacga
       61 cgcccacgac ctggccctgt ccgcctcgct cgcccgctcc tccaaggacc tcgaggagca
      121 tgagtatgcg gtgcgctcgg tcgccgatgc cctcggccgc cactgcgcct cgatgaacgt
      181 cccggagagc ccgttcgtcc tccacctgcc caacgtgatg cacctggcca ccgatgtggc
      241 cggcgccgtc gaggacggcg ccacctcgct cgaactcgcc gcgagcctgc acccctccgc
      301 cgccgtctgc ggcaccccca ccgccaccgc cgacgccctc atcaccgaac tcgaaggctt
      361 cgatcgcggc cgctacgccg gccccgtcgg ctggatggac gccgcgggcg acggcgagtg
      421 ggggatcgcg ctgcgcagtg gacaactcga tccggcggat ccgtgccaga tcagcatttt
      481 cgccgggtgc ggcatcgtcg ccggctccga ccccgaggac gaggtcgccg agtcggacgc
      541 gaaattcctc cccatgcgcg acgccctcac ggccgcgccc acagcctgac gactcaggca
      601 tcccgtgacg acccagtgat gagtcgagtg agcgcgacac gcccataatt ggtcccggac
      661 atgggcgtgt cgcgctcact cgacttgagg tggtgtgggg ccgcactgcc gggtcaaggc
      721 ggccgcgact cgcgcgagga aggcccgctg ccgagccccc ggatcgagat cttcggccca
      781 catcgggaac accgcgcatc tgttgtcctc gaggatgcgc tgacgcaatt cgtcagcgct
      841 ggcctggcgt cggtcagcgt ggtgcgctcc ttgatactcg acgaccaccc acccgtcgcg
      901 ggtgcgccac accaggtctc cttcgccgag ccatccgctt ccgtccgggg cggtgatgtc
      961 gtgattgatc aacggctccg gcaggccggc ctcgacgacg accagccggg cctcggtctc
     1021 cttgggggag cgcacgccgg gccggatccg ctcccgtgcc cagagcaggt ttcgtttccc
     1081 ccgtggccgg atccgtcgag cgatggccgc gtcgatctcg cggtatgccg ccgccacgag
     1141 tgccgcgtcc aggtacggct ttcgccgagg cgtcagttgc gcagcgttcg cgaggcagag
     1201 cagtcgattg agcacctcat cgccgacgac aaccaggtcc acgcgggtca tcgtggggtg
     1261 gcgccccacg gtcagagcgg ccagatcgat ccacgtgtcg acgaggtcga ccacccgcag
     1321 gccgtcgacg accacgacct ggcgggtctc caaaccgcgg cggccgaccc agccgtcccg
     1381 tcgtcgctgc ccgacgtcgg tgcgggtgca gacatcgagc ctgcgctgct gcagcgcgcg
     1441 tgggggcaac ggcaggacca gcagccgcgc cccggtcaca tgtgagaaag cggcgtcccc
     1501 gcacatggcc aagtgggcag cccgggcgcg ctcgcgcagg tcgtcgccga cggggtgctg
     1561 ggcccacacc ccgcgggtcg ggtgttcgag gcgggaggtg cgcagcgcct tgctcggcac
     1621 gccgagtgcc cgggcgcgat cgctggtgaa cgcaacgccc gccagctctt cgggcagctc
     1681 tatcggcctc atacccgtca agcgtggcgg caccatccgc cgcgcacccg tcgctatcca
     1741 caagggccag tcgagtgagc gcgacacgcc cagaattcga cctgaacatg ggcgtgtcgc
     1801 gctcactcga ctcgagggac gccgtgggac agccgtctcg accaggatca tcttaggtaa
     1861 gccttacttc acggccccca accatgctga cctagactca gcaggttcgt gaaaccgttc
     1921 acaagcctcc cgaggatccc ccctgtgtct gagactgccg agaaggccga cgtcatcgtc
     1981 gtcggcgccg gcccggcggg gtcgtcgacc gcggcctacc tcgcgatgtc cggcctggac
     2041 gtgctgctgc tcgagaagac cagcttcccc cgcgagaagg tctgcggcga cggcctgacc
     2101 ccgcgcgcgg tcaaggaact gatcaccctc ggcatcccca cccccgaaga agaggggtgg
     2161 atccgcaaca agggcctgcg gatcatcggc ggcggtatgc ggctgcagct ggactggccc
     2221 gacctggcca gcttcccgcc atacggcctg gtccgcaccc gccaggactt cgacgacatc
     2281 ctcgccaagc acgccgtcaa gcacggcgcc cgcctgcgcg agtccaccaa tgtcaccggc
     2341 ccgatccgtg acgagagcgg ccgcatcacc ggcgtcaccg cgaaggccag cgacgagaag
     2401 gggcgcgccg tcggtccgga gcagcgtttc gaggcgccgc tcgtcatcgc cgccgacggc
     2461 aactcctccc gactgtcgat ggcgatgggc cgcgagaagc gcgacgaccg cccgatgggc
     2521 gtggccgtgc gtacctatta caccagcccc cgccacgacg acgactacct cgagtcctgg
     2581 ctggagttgt ggtccagcga cgacgagggc aacaagatcc tcctccccgg ttacggctgg
     2641 atcttcggcg tcggtgacgg cacctccaac gtcgggctcg gcatcctcaa cacctccagc
     2701 gccttcggca agaccgacta caaggacgtc atgcggcgct gggtcgcgac gatgcccaag
     2761 gagtggacct acagcgacga gaccatggtc ggcccgatcc gcggagccgc gctgccgatg
     2821 ggtttcaacc gccagccgca ctaccgtgac ggtctgctcc tcgtcggcga cgccggcggc
     2881 atggtcaacc ccttcaacgg cgaaggcatc gcctacgcga tggagtccgg gcgcctcgcc
     2941 tccgaggtca tcgcccaggc cttcgcccgc ggcgacgacg ccggccgcga gcgggtcctc
     3001 gcgtcatacc ccgcagtcat gaaggacgac ctcggcggct actacaccct cggccgctgg
     3061 ttcgcctccg cgatcggcca ccccgaggtg atgcggctgg cgacgaaata cggcctgccc
     3121 cgcaccacct tgatgaagct gctgctcaag atcatggcca atctcgctga gccccgcggc
     3181 ggttcggcct ccgaccggct gatcgtcgcc ctgtccaaga tggctcccga cgcctgatgc
     3241 tcatgtcgtg caacgctcga accggcggcc cccctaggat ggcggaggaa gcgcccgtga
     3301 cctgccccac gtcgatggaa aggggagttg gctgatgcac aacccctatg tcccgttgct
     3361 gttcttcctg gcgatcggtg ccggcttcgc gatcttctcg gtgggcatct cgcccatcac
     3421 cgggccgcgg cgatacaacc gcgccaagat ggacgcctac gagtgcggta tccagcccag
     3481 cccgcaggcc caaggcggcg gccgggtgcc gatcaagtac ttcctgacgg cgatgctgtt
     3541 catcatcttc gacgtcgagt ccgtcttcct ctaccccttc gccgtcgcct tcggcggcct
     3601 gggactgttc gcgctcgtgg agatggtgct cttcatcctc accgtcttca tcgcctacgc
     3661 ctacgtctgg cggcgcggcg gcctggactg ggactgacag gagccactga cacatgggta
     3721 tcgaagagaa actccccgcc ggatttctgc tcagcaccgt cgagaacgtc gccggcctgc
     3781 tgcagaagag ttcgatctgg ccggccacct tcggcctggc ctgctgcgcc atcgagatga
     3841 tggccgtcgg cacgcccgac tacgacatcg cccgcttcgg gatggagaag ttctccgcct
     3901 ccccgcgaca ggccgacctg atgatcgtcg ccggccgcgt cagccagaag atggcgccgg
     3961 tcctgcgcca ggtctacgac cagatgccca accccaagtg ggtcatctcc atgggcgtgt
     4021 gcgccagctc cggcggcatg ttcaacaact acgcgatcgt ccagggcgtc gaccacatcg
     4081 tccccgtcga cgtctatctg cccgggtgcc cgccgcgccc ggagatgctg ctcaacgcga
     4141 tcctgacgct gcacgaccag atccgccact ccaagctcgg cgtcaaccgc gtcgccgccg
     4201 cccgcgccgc cgagaaggcc gcgctcgagg ctaccccgac ctcgctgatg gccccgaccc
     4261 acgaccacct ggccggaaag agcctgctgg catgaccgag acccccaagc ccgccgacca
     4321 gccccccgcc gagccggcca agcccaacga gccggccatc gaccgggccg aaaagacgat
     4381 cgagaccccg cccgtccccg gcgccgagaa tcttccggcc cagcccggca ccacccccga
     4441 gccggtccag cgcggcgagc gcaccggcat gttcggcgct cacgacaccg gtgacacgac
     4501 cggatacggc ggcctgcgga gcccgatcct ctatcccgga gcagccgtgc gcccctacgg
     4561 cggcgacttc gacgccatcg ccgacgccct cgaggctgcc accgatcagg gccagatcgc
     4621 ccgcaccgtc atcgaccgcg gcgagatcac cttcgtcatc ccgcgcgagc acctgcccgc
     4681 cgtcgtccgc gccctgcgcg acgacccggc gctgcgcttc gagttgtgcc tgtccgtctc
     4741 gggcgtgcac ttcccgagcg acatcggcaa cgagttgcac gtcgtctatc acctgatgtc
     4801 gatcaccaac ggccaccggc gcatccggct cgaagtgacc gcccccgacg ccgacccgca
     4861 catcccgagc gtcgtcggca cctatcccgc cgccgactgg cacgagcggg agacgtggga
     4921 catgttcggc gtcgtcttcg acggccaccc cgcgctgacc cggatcctca tgccggacga
     4981 ctggcccggc cacccccagc gcaaggacta cccgctcggc gggatcccag tggaatacaa
     5041 gggcgggacc gttcccccgc cggatcagcg gaggtcctac tcataatgac gaccccacac
     5101 agcgagagcg gcagcaccgt ctacaccgcc gccgatcccg acgaggtccg ccatatcgac
     5161 gacctcgacg agcaggccga cctgtatgcc gcccccggcg aaccgtccga gggtcgcgtc
     5221 ttcaacgccg tcggcggcga ctggaacgac atcgtcggcg aggtcggctc ccgccccgag
     5281 gagcgcatcg tcgtcaacat gggcccgcag cacccgtcga cacacggcgt gctccggctc
     5341 atcctcgaga tcgagggcga gacggtcacc gaggcacgcg ccggaatcgg ctacctgcac
     5401 accggcatcg agaagaacat ggagttccgc acctggaccc agggcgtgac cttctgcacg
     5461 cgcatggact acctcatgcc gatcttcacc gagacggcct actgcctggc cgtcgagaag
     5521 ctgctcggca tcaccgacca gatcccggcc cgcgccagtg cgatccgcgt cctgatgatg
     5581 gaactcaacc gcatcggcag ccacctggtg tgcctgggca ccggcggcat ggagctcggc
     5641 gcgaccacgg tcatgacgat cgggttccgc gagcgtgagc gcatcctcaa catcttcgag
     5701 atggtctccg gcctgcgcat gaacaacgcc tacgtgcgcc ccggcggcgt cgcgcaggac
     5761 ctgcccgccg gcgcgatcca ggccgtccgc gacatcatcc ccgaactgcg ccagggcatc
     5821 cgcgagctcg aactgctcat gctcgagaac cccatcctca agggccggct ggtcggcatc
     5881 ggctatctgg acctgaccgg ctgcatggcg ctcggcatca ccggccccat gctgcgcagc
     5941 gccggttacc cgctcgacct gcgcaaggcc acgccctact gcggctacga gaaatacgaa
     6001 ttcgacgttc ccacccgcac cggtgccgac gcctacgacc ggctctggct gcgcgtgaag
     6061 gagatgtggg agtcgctgaa gatcgtcgag caggtcgtcg ccgaactgca ggacaacccc
     6121 ggccccgtga tggtcgagga caagaagatc gcctggccgg cgcaactgtc cgtcggcccc
     6181 gacggcatgg gcaacagccc cgcgcacatc gccgagatca tgggcacctc gatggagtcc
     6241 ctcatccacc acttcaagct cgtcaccgag ggcttccgcg tcccgccggg ccaggcgtat
     6301 gccgcggtcg agtcccccaa gggcgagctc ggctgccacg ccgtctccga cggcggcacc
     6361 cggccctatc gcgcgcattt ccgcgagccc agcttcaaca acctgcaggc cgtggccgca
     6421 ctgtgcgaag gcgcgcagct cgccgacgtc atcgtcgccg tggcctccat tgatcctgtg
     6481 atgggtggag tcgaccgctg atggagcact acggcgaaca aaccgcgtcc ggtttcctgc
     6541 acgtctccgc agagagcaag gagccgtatg ccgcggacgt cctggcgaag ttgcatcagg
     6601 acgccgaggc ggtcatcgcg cgctatcccc agaagcggtc cgcgctgctg ccgctgctgc
     6661 atctggtgca gagcgtcgac ggctatgtgg ccggccgcgg cgtgagattc tgctccgagg
     6721 tcctcgggct gtccgaggcc gaggttagcg gtgtcgcgac cttctacacc cagtacaagc
     6781 gccaccccaa cggcgactac accgtcggcg tctgcaccaa cacgctgtgc gcgatcatgg
     6841 gcggcgacaa gatctgggac accgtcagcg agcacctcga cctcgaacac gacgagacca
     6901 gcagcgacgg caagatcact ctcgagcgcg tcgagtgcaa tgccgcgtgt gacttcgccc
     6961 cggtcgtgat ggtcaactgg gagttctacg acaaccagac ccccgaatcg accgtgcaac
     7021 tggtcgacga cctgcgcagc ggcgccccgg tcaagccgac ccgcggcccg gaccgccagt
     7081 gcaccttcaa ggagatgtcc cgggtgctgg ccggcttcca cgacggcctg gccgaccagg
     7141 gcgtcggcgc cggcagcgcc tcgctcgccg gtctgcgcgt cgcccgcgag cagggctggg
     7201 tcgcccccgg agacgccggc tccgactcgg gcagcggccc ggtggccgac gttgcccccg
     7261 tcgccgacag cgccctgcag ggcgagcggg acaagatgac cgatgtcgcg ggcgagtccc
     7321 tgcagcgcgc ccagcagacc gagcaggcag gggagtgacc cgatgacgca gctcacgccg
     7381 atcttcaccc gctactggga ccagccgaac tcctggacgc tggagaccta cgccaagcac
     7441 agcggctacg agggcctcaa gaaggcgctg ggtatgccgc ccgccgacat catcgcgagc
     7501 gtcaaggact cgggtctgcg cggtcgcggc ggtgccggct tcgccacggg cgtcaagtgg
     7561 ggcttcctgc ccccgccgga cggcgggccc cgttatctcg tcgtcaacgc ggacgagtcc
     7621 gagccgggca cgtgcaagga catcccgctg atgatggcga caccgcactt cctcatcgag
     7681 ggcgtggcga tcacctcctt cgccatcggc tgcaaccacg cgttcatcta cctgcgcggc
     7741 gaggtcgtcc acgtctaccg ccggctgctg cgcgccgtcg aagaggcgta tgccgcgggc
     7801 cacctgggca agaacatcca cggctcgggt ttcgacctcg acgtcaccgt gcacgccggc
     7861 gccggcgcct acatctgtgg cgaggagacg gcactgctcg acagcctcga gggccgccgc
     7921 ggccagccgc gcctgcgccc gccgttcccc gcggtcgccg gcctctacgc ccgcccgacc
     7981 gttgtcaaca atgtcgagtc gatcgcctcg gtcgccccga tcctgctcta cggcgacgaa
     8041 tggttctccg ccatgggcac cgagaagtcc aagggctacg ggttcttctc cctctccggg
     8101 cacgtcaaga accccgggca gtatgaggcg ccgctgggca tcactctccg tgagctgatc
     8161 gacatggccg gggggatgcg cgacgagaac cacccgctga agttctggac cccgggtggt
     8221 tcctcgacgc cgctgttcac ccaggagcac ctggacgtgc cgctcgactt cgactcggtc
     8281 gctgcggccg gttcgatgct cggcacccga gcgctgcaga tcttcgacga caccacgtgt
     8341 gtggtgcgcg ccgtcgaccg gtggaccgac ttctacaagc acgagtcctg cggcaagtgc
     8401 accccctgcc gtgaaggcac ctggtggctg aagcagatcc tcggccgcct cgagcacgga
     8461 cacggcgacg aggaggacct ggagaagctg ctcgacgtct gcgacaacat cctcggccgc
     8521 agtttctgcg cgctcggcga cggcgccacc tccccggtca ccagctcgat ccagtacttc
     8581 cgcgacgaat acctcgccca cctgacgcag ggtggctgcc ccttcgaccg ggatgcctcc
     8641 gcagtcttcg cgaaggagaa cgcgcacgca tgagcgtcac ccccgcccag accgatctgg
     8701 tcaacctgac catcgacggc ataccggtgt cggtgcccaa gggcacgctg atcatccggg
     8761 ccgccgagca gatcggcatc cagatccccc gattttgcga ccacccgtcg ctcgacccgg
     8821 tcggcgcctg ccggcagtgc ctggtcgagg tcgccatgcc cggcccgccc ggaccggacg
     8881 gcaagcccag cgccccgcgc cagatgcagg gccctcccgg gcggatgaag ccgcaggcct
     8941 cctgcaccat gacggtcgcg gagggcatgg aggtcaagac ccagtacacc tccgagggcg
     9001 ccgacaagtc gcagcagggc gtcatggagc tgctgctgat caaccacccg ctcgactgtc
     9061 cggtctgcga caagggcggc gagtgccccc tgcagaacca ggcgatgagc agcggccggg
     9121 cggtctcgcg cttcgaggac gtcaagcgga cctatcccaa gccgatcaat atctccagcc
     9181 aggtccttct cgaccgcgaa cgctgcgtgc tgtgcgcccg ctgcacccgc ttctccgacc
     9241 agatcgccgg tgacccgttc atcgccctgg tcgagcgcgg cgcgctgcag caggtcggca
     9301 tctacgagga gcagcccttc gagagctact tctccggcaa caccgtgcag atctgcccgg
     9361 tgggcgcgct gaccggcgcg gcataccgct tccgcagccg tccgttcgac ctggtctcca
     9421 ccgccggcgc ctgcgagcac tgcgccagcg gctgcgcgat ccgaaccgac caccgtcgcg
     9481 gcgtcgtgct gcgccggatg gcgatcaacg accccgaggt caacgaggag tggaactgcg
     9541 acaagggccg ctgggccttc accgcaccca ccgtcggtga ccgcatctcc ctgccgatgg
     9601 tgcgcggcgc ttccgggcag ctgcaggtcg cctcctggcc cgaggccctg gccgccgcgg
     9661 ccgccggcct gcagggtcag agcgccggtg tcctgaccgg cggccggatc agcgtcgagg
     9721 acgcctacgc ctacggcaag ttcgctcgca cggtcctcgc caccaactcg atcgacttcc
     9781 gggcccgccc ggccagcgcc gacgaggccc gattcctcgc cgcgagcgtg gtggcgaccg
     9841 gccccgccgg cggcagcgtg acgtatgccg cgctggagaa ggccccgaag gtgctcctcg
     9901 tcggcttcga gccggaggaa gagagcccta tcgtcttcct gcggctgcgg aaggccttcc
     9961 gcaagaacaa gacccagatc tcctccatcg ccccgttcgc cacccgcggg ctgggcaaga
    10021 tgggcggcac ctggatcccc gcggcccctg gcaccgagac cgagatcctc ggttcggtgg
    10081 acgctgcggc ggcgctcgac ggcgaaggca gcgtcctgct cgtcggtgaa cgcctggcca
    10141 ccgtccccgg tgcgctggcc gccgccaccg ctctcgccga gcgcaccggc gccaccctcg
    10201 cctgggttcc ccgacgggcg ggggagcgcg gggccctgga ggccggcgcg ctctacaacc
    10261 tgctgcccgg tggccgcccg gccggcgacg ccggcgcccg ggctgacctc gccgccacgt
    10321 ggcaggtcga cgctctcccg ggcgacaccg gcctcgacgg cgccggcatc atcgccgccg
    10381 ccgcctccgg tgagctgggc gccctggtcg tcggcggcgt cgacgcgagc gacctcggcg
    10441 acggcgcggc atacgacgca ctcgagaggg ccttcgtcgt ctccctcgaa taccgcccca
    10501 gcagcgtcac cgcggccgcg gacgtcgtcc tgccggtggc gccgcacgcc gagaaggccg
    10561 gcaccttcgt cgactgggag gggcgggtgc ggccgttcgc ggcggcactg gagaccaacg
    10621 ccctctccga ctaccgggtc ctcgacatgc tggcgagcga gctcgggcac ttcctcggcg
    10681 cgcgcagcct cgagcaggtg cagaccgaga tgagcacgct cgggccgtgg tccggtgccc
    10741 gcgcgagcaa gcctgccgcc ctcccggcgc aggtgccctc gacccggccc ggccagttcg
    10801 tcctcgccac ctggcaccac ctgctcgaca agggccgcct ccaagacggc gaacccttcc
    10861 tcgccggcac cgccccggcc gccgtggccc ggatctccgc atccggggcg caggcgctcg
    10921 gtgtccacga cggcgagcag gtcacggtca ccggcccggc gggcagcgtg accgtgccgc
    10981 tggcggtgac cgagatggtc gacaacgtcg tctgggtccc gaccaactcg gccggctgcc
    11041 gcgtcgccgt cctcggcgtc ggcgccggct cgctcgtcac cgtcaccccc ggaggtgcct
    11101 cgtgaacgtc ttgaccgtcc tcgccgcggc ggagccctat gtgccgcgcg acaacccggt
    11161 cgccgacttc agcgacaccc ccgtgtggct gtcgctggtc aaggcgctct tcctgttcgt
    11221 ctatctgctg ctcagcacgc tgctcgtcat ctggttcgag cgacgcgtca tcggccggat
    11281 gcagcagcgc cccggcccga accgcttcgg tccgttcggc atctttcaga ccctcgccga
    11341 cggtgtgaag ctggcgctca aggaggacat cgtccccaag aacgccgaca agctgatcca
    11401 cctgctcgcg ccgatcctcg ccgcgtcgat ggccttcgtc tccttcgcga tcatcccgtt
    11461 ggccggcaac gtgcggatgt tcggccacac cacgccgctg cagctgaccg acgtgccgat
    11521 cgcggtgctc atcgcgttgg cggtcgccgg cgtgggtgcc tacggcgtcg tgctcgccgg
    11581 ctggtcgtcg ggctcgacct atccgctgct cggtgggctg cgctccagcg cccagatcat
    11641 cagctacgag atcgccatgg gtctgtcgct cgtcgccgtc ttcctctaca gcggttcgat
    11701 gtcgacctcg cagatcgtcg ccaatcagcg gagcatctgg cacgtcatac cgctggtcgt
    11761 ctccttcgtc atctatgtca tcacgatggt cggcgagacc aaccggttgc ccttcgacct
    11821 cgccgagggt gagggcgagc tgacagccgg gttcatgacc gaatacagct cgatgaagtt
    11881 cgcgatgttc ttcctcggcg aatacatcaa catgttcacc gtcgccggcc tggcgaccac
    11941 cgtcttcctc ggcggctggc aggccccgcc cggcatcgcg gccatcaacg acggcatgtt
    12001 caaccacggc tggtggggcc tgctgtggtt caccgtcaag ctgtggctgt tcatgttcct
    12061 cttcgtgtgg ctgcgtggtt cgctgccccg cacgcggtat gaccagttca tgcgcttcgg
    12121 ctggaagttc ctcatccccg tcacgatcgt gtgggtcgtc gcggtcgcct tcctgcaggc
    12181 cgccgaccac ggttacctcg gagagaaggt cgtctcgatc ttcggtgccg agcgtcgcct
    12241 ggcctcgctg atcgtcatcg gcgcgctcgc cctgctggtg ctggcggcga cgtggatctg
    12301 ggaaggccgc caggctgcca agatcgaggc ggccaagccg gtcgtccccg acgcgatcga
    12361 tccgtatgcc gggggcttcc ccgtgccgcc attgcccgga cagcgcctcg aacaggccgc
    12421 tcccgccccg accaaggaag tgctccatgg ctgaggacaa gcccggcttc ttcagtgaga
    12481 tgttcgcccc cgtcggcgga ttcggcgtca ccttcgcgac catgttcaag aaggtcaaga
    12541 ccgtcgaata tcccgaggtc aagcgcccca cccagccgcg tttccacgga cgtcaccagc
    12601 tcaatcgcta tcccgacggg ctcgagaagt gcgtcggctg cgagctgtgc gcatgggcgt
    12661 gcccggcgga cgcgatcctc gtcgaaggcg ccgacaacga cgacaccccc ggcggcaccg
    12721 gccgcttctc ggcgggtgag cggtacggcc gcgtctacca gatcaactat ctgcgctgca
    12781 tcttctgcgg cctgtgcatc gaggcctgcc cgacccgcgc gttgacgatg accaacttct
    12841 acgagctggc cggctacaac cgcgccgacc tgatcttcac caaggagcag ctgctggcgc
    12901 ccttgcaggc cgggatggac gagccgccgc acccgatggc cgagggcctg tcggagcgcg
    12961 attactacct cggaaaggtc gaagggggtg ccaagtgacc agcaccgctg aagccgtcac
    13021 gttctggatc ctcgggtcca tcgccgtgct catgagtctc ggcctgctgt tcgccaggaa
    13081 ggccgtgtat gccgccatgg gcgtcgcggt cacgatgacc atcctcggca tcttctatgt
    13141 cgcgcagggc gccccattcc tcggggtcgt gcaggtgttc gtctactccg gcgccgtgat
    13201 gatgctcttc ctgttcgtcg tcatgctcgt cggtgtcgac tcctccgaca gcctcatcga
    13261 gaccatcaag ggccagcgct ggatcgggct gctgctctcg ctcggcctgg ccatgctgct
    13321 cgccgggatg atcggcaagg tcaccatcga cggcaccccg accctcgacg cggtcaacca
    13381 gggcggccac atctcggcga tggccaatgt catcttcggc aagtacgtct gggtcttcga
    13441 gatcacctcg gccctgctca tcaccgccgc cctcggcgca atggtgctcg cccaccgcga
    13501 gcgcctcggc gccaagccga accagaaggc ctggtcgcag gagcgcttcc gccgcggcga
    13561 gtacgtctcc ggcctcccgg cccccggtgt gtatgcacgg cacaacgccg tcgacacccc
    13621 ggcgctgctg cccgacggca cacccaccga gctgtccgtc tcgcgcaccc tgcaggcccg
    13681 cgaccaggtc gccctgcccg gccagtacac cgccgccgaa cagcagctgc agaaggagat
    13741 cgaggagggg cgcacgcgat gagcctgatg aactacatct acctggccat gatcctgttc
    13801 gccatcggcg cggcgacggt gctcacccgc cgcaacgcga tcatcgtgtt catgggtatc
    13861 gaactgatgc tcaactcggc caacctcgcc ttcgtcacgt tcgggcggat gcaccagtcc
    13921 atcgacggag cggccatcgc gctgttcgtc atggtcgtcg cggccgccga ggtcgtcgtc
    13981 ggcctggcga tcatcatggc catcttccgt gcccggcgtt cggcatcggt cgacgacgcg
    14041 aacctgctga agtactgaga aggggataac acaacgtgtc caccctcctg acacagctgg
    14101 cactcgaaac cgccacgtcc tcggacgcgg tctcgatgcc cggctggtgg ctcatcgccc
    14161 tgccgttggc cggtgccgcc ttgttgctgc tcggtggcag ggccaccaac agcttcggcc
    14221 ccgccctggc caccggcttg tcctgggcca gcttcgggct cggcgtcgtc gcgctgctgc
    14281 agttgctggc cgtcgacccc gaccagcgct cgacccacca gacgctcttc aactggatcc
    14341 ccgccggtgc cttccagctc agtgccggcc tgcagatcga caccctgtcg gtcagcttcg
    14401 tgctgctgat caccttcgtc ggttcgctga tccacgtcta ttcgctcggc tacatgagcc
    14461 acgaccccga caagcggcgc ttcttcgcct acttgaactt cttcgtcgcg gccatgttgc
    14521 tgctggtgct ggccgactcc ttcctgctgc tgtttgtcgg ctgggagggc gtgggcctgg
    14581 cctcctacct gctcatcggg ttctggaaca cgcgggaggc cggagcgatc gccccgggcc
    14641 acttcgcgga ggccaacgcc gccgccgcca acaaggcgtt cgtcgtcaac cgggtcggcg
    14701 acatcggcct gtcgctggcg atcgcgctga tgttcgccac cttcggtgcc gtcgacttcg
    14761 ccgcagtcaa cgccggggtc gccgcgggcg gcgccggcac ggcgacgctc accgccatcg
    14821 gtctgctgct cctgctcggc gcctgcggaa agtccgcgca gttcccgctg cagagctggc
    14881 tcggcgacgc gatggccggc ccgacgccgg tgtccgcgct gatccacgcc gctaccatgg
    14941 tgaccgcggg tgtctacctc gtcgtccgca gccataccat cttcgaagcc gctcccaacg
    15001 cccagctcgc cgtcgccatc gtcggcgcga tcacgctgac atacggtgcg atcgtcgggt
    15061 gcgccaagga cgacatcaag aaggcgctcg ccgcctcgac catgtcgcag atcggctaca
    15121 tgatgctcgc cgcgggcctg ggcccggtcg gctacgcctt cgcgatcttc cacctgatca
    15181 cccacggctt cttcaaggcg gggatgttcc tcggcgccgg ctcggtcatg cacggcatga
    15241 gcgaccaggt cgacatgcgc cgcttcggca acctgaacaa gttcatgaag atcacctgga
    15301 tcaccttcgg cctcggctgg ctggcgatcc tcggtgtgcc gcccttctcc ggcttctgga
    15361 gcaaggacaa gatcatcgag gccgccttca tcggcgaggg ctggcgcccc tgggtcttcg
    15421 gcctggccgc gctgctcggc gcgggcgtca ccgcgttcta catgtcgcgc ctgttcttca
    15481 tgaccttcca cggcaaggag cgctggaccg acgagccgaa acagcacccg cacgagtcgc
    15541 cgctgacgat gaccctcccg atgatggtgc tcgcgctggg ctctgccggg ctcggcctga
    15601 tcctcggccc gacggacatc ttcaccaaat ggctcgagcc ggtcaccggc caccccgagc
    15661 atggcgagcc cgtcctgtcc gtgccggtcc tgatgacgct gaccctgctg ctggtcgccc
    15721 tcggtgccgg gctcgcgtgg ctgcggtatg gccgtgacga ggtcccgatg tacgctccgg
    15781 cgggcaatgc gctcacgcag gccgcccgca aggatctcta ccaggacgac atcaacgagg
    15841 ccgtcttcat gtggccgggc gtcaaactgg tccgctcggt gatctggacg gacaacgagg
    15901 tggtcgacgg cggcgcgatg ggtctgggca aactgaccgg cggcaccgcc acctggctgc
    15961 gccgcatcca gaacggctat gcacgctcct acgccttgac gatgcttgcc ggcatcgtcg
    16021 ccgtcctcgg tgcactgtgg gtgatgaact gatggataac ttcccctggc tgaccgcgat
    16081 gatggtcgtg ccgctcatcg gcgcggcctt gatcgcggcg ctgccctcgg cgatggcggc
    16141 ccgcgccaag gagctggccc tggccttctc cgtcgcgacg ctcgccctgg gtgtcgg
//
LOCUS       DAOPJG010000061        16164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3804_length_16164_cov_8.046434, whole genome shotgun sequence.
ACCESSION   DAOPJG010000061 DAOPJG010000000
VERSION     DAOPJG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 16164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16164
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3804_length_16164_cov_8.046434"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PKU75_07535"
     CDS             complement(<1..848)
                     /locus_tag="PKU75_07535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPF80764.1"
                     /translation="MPATGPDIAGHRHLRLISDAGGYGDVHLYEEIALGRRVAVKVIR
                     EADVGSGTVAQFLAEANTMARLEHPNIVRVYASGQTSDGRPYISMQYCADDTYEQRLE
                     RERLSVAEVLSTGVRIGSAVETAHRAGLLHRDIKPANILKTPWGAPGLTDFGVAHQIS
                     GDDTASSDVGVSIPWSPPEMLYTSTNGSPASDVYSLAATLWHLLVGRPPFDLPSGDNS
                     LMATMARIRDLPPPATGRPDVPETFERVLRQALAKNPQHRPLTMAEFVRNLQSVERDL
                     RLPVTEA"
     gene            complement(848..1351)
                     /locus_tag="PKU75_07540"
     CDS             complement(848..1351)
                     /locus_tag="PKU75_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPF80765.1"
                     /translation="MNRAALQQPPTVPAGPTVWAAHCPQGHPSQAHAAVCRVCGVAMP
                     PDPVPREIPRPVLGRLVLPTGDPVPLDSDLVLGRDPQVPDGVTPPLPRLVVLNDPRFE
                     VSGQHATVTLNFWDVCLTDLGSTNGTEVVTPDGRRQRLAPDSPVTIAPGTRIILAEVF
                     EMTFEAT"
     assembly_gap    1571..1670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1671..2480)
                     /locus_tag="PKU75_07545"
     CDS             complement(<1671..2480)
                     /locus_tag="PKU75_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80766.1"
                     /translation="MTGETSLTFAPGAKAACAKGSLVVLIDRPLTDPVALSTWTLLDE
                     GQGADLVVGTFLSHGLADAPDFGLVEVGSDGGARVVVRGRATARGVDATGPFHVTATG
                     ILADERHEGIVWCQVQVDDASPEAGVPLVAGVLPASAVRISRGGAVAEHGLAAVVAGP
                     VDEAEPVQGTEPVERAEPVHEAEPLHEAETAEVEDVDPGETPMPVHPPVPGGEWQVAH
                     DDLPGPNPYAHLFGSPPSPQEEAPAVQGAPVSEPLLAPAAPSVVEPAPVVEP"
     gene            complement(2477..3217)
                     /locus_tag="PKU75_07550"
     CDS             complement(2477..3217)
                     /locus_tag="PKU75_07550"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPF80767.1"
                     /translation="MRTLNEDAWYAGEQLFVVADGMGGHRCGDVASTLTIDTVRSLDR
                     EDVSEDEVRQVVRRANAAVLGHSAENPEVAGLGTTLCGAIRRRDDWLVFNVGDSRCYL
                     LTSGSLRQVTVDHSEVQEMVDRGLISRDEARVHPDRNVITRAIGQVPPPEVDIVTLPA
                     GHGGTLLVCSDGLPSEVSDADIAGLCSGDPPLDRLAEELVAAAEAAGGHDNITVVLVT
                     DVTDAPALRPERARTTLPRDLVIREVAQ"
     gene            complement(3260..4081)
                     /locus_tag="PKU75_07555"
     CDS             complement(3260..4081)
                     /locus_tag="PKU75_07555"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPF80768.1"
                     /translation="MDRHVHFVVSSEGQMDAGSAGQTCSMCALELDSRATRCPRCGQQ
                     VWASGPAAPAADLTGAVTPPEVPAPGIPTPGRTVQRSQLPVAQPAPIAPESPFPPAGV
                     ATPSATTGPSAGSPTSSVAPVDLGTRFLANLVDSVFLFVVFMVLAIVITVLSQVSNVL
                     GLLGQLVFMLIGLGYYALGIGRGGATIGKRIMGVSVVDLGSHQPIGVGRALGRYIGLG
                     LMALPCYLGYISFFTDKSGYNRAFHDQIVSSVAIAAPKESFGQAARHYLDAVRGG"
     gene            complement(4170..5135)
                     /locus_tag="PKU75_07560"
     CDS             complement(4170..5135)
                     /locus_tag="PKU75_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80769.1"
                     /translation="MQCPSCQEPVAIWAAFCPHCGTSLAAPAPEQPAERESLTSELFG
                     SDDLRDTVERPRPEGAPDGESVAGESVAEEQYASEPYAEEPYTPEPYAAELIPTDPAP
                     PAVAPTEALPVGTEPAPSTPALGAASAAGAGAAYGQGSPAGEPVWDDEPPRRSGATAL
                     LVAAGLVVALTLGFALWSIVRVSDTGDASSSPSAQTTTSPRGTPEGTSATPETSPPAS
                     PGQTPSTSPSKAAAGPVPAGAVACGVTGSGETAAVFGADQNTTCPFAQNVAAAYRATG
                     MTDGGSVTVQATSPVTGKDYTLTCTGRLPTTCVADTGATVYLTNG"
     gene            complement(5243..11599)
                     /locus_tag="PKU75_07565"
     CDS             complement(5243..11599)
                     /locus_tag="PKU75_07565"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPF80770.1"
                     /translation="MNPFGTRAPAGSPAAARSARRGRIASLTVVALAATSLGVAAATS
                     RGSTVHEADLGDGGIWLTSDALAKFGRLNVQAGQLDAGVSADVEPGSSIDVLQDGAAI
                     LTWSRATQQLQALDPKTSTTTSATAIVPAQAAPTAGVAPTSVVDLRGGTVALVEEKSG
                     KVWAQRVDDRAGITDLSKLGTTSKPIATVGANAALAVGEDGAVHVVSGAKSTMTTIRP
                     GGQAFAAPVTTEIGLKGAGIQVSALGKDWVAYDPTTDRLASAGKPDGVKAGLATDGDH
                     AAYGLLQVPGPAADAVAIAGTAAVRMIRLDGDPAPGGIEVQERVNRSAEVPHVAAPVV
                     LKGCVHGAWNEANKVFYGANCGVANPVPTGTIENISDKAIRSGVKFRVNRGAIVLNDL
                     DSGAAWDVDRDQQKKIDNWDSLIPPPQTENDNDKKDKNLVDDAVLEQPPTAMPDSLTV
                     RAGRTSKLHVLDNDTDVAGSVLAIDPTDLSKPSDEKVGTSVAADGQTVDVTVPAGHKD
                     SFTFTYMVGNGKAKSAATTVTVAIVGDEVNTPPRLREGSKDLARSAYPVLAGRRISLP
                     VIGDWRDDQSDTVSASAADAGSSVDGQGRVEYLSGMEPGVVSVPYTVDDGQGGTTPGS
                     VDVEVVGPLDSRPRNPVTQPDVIRGVVGKPIQVEPLSNDIPGADPGDPEAEMRLAGDV
                     QPVGNLTVDTNKDTGVVTITPAAAGTFILSYAAQVGSGVAPGRIRIDVIGDPDPNAPP
                     IAVSDAGTLRGQLSSMVDVLANDYSPRADVMVTQSVSVESSSAWIEASIYQGRWVRLR
                     SLEPATGGDGFARPGTVNYSVSDGTRTAKGQISVVQKAVTPITPVVRDDKAIVREGDA
                     VSIPVLDNDTMADGIPLRLDPLSVKVLDGREQTAFASDNLIRFVPTASGLTADDHVTI
                     EYAAYPDGDPQRAQTGRVSVAVTPQPTTARPNQAPVARSFTTSVTAGDPLTITVPTSG
                     VDPDGDTVSIRGIVGRDGGAVSLRYGRIVSVGPDTIRYEAYPTAAGTEVIDYEVADRF
                     GELSRAFVRVGVVAPGDPQPPVAVDDTVTAAPGKTVTIPVLQNDLIARGDVVDLTLGD
                     TEGWRVDEETRMVSTVVPEETAPLHHTVYTIDNGMFDPSRASILVRGRKDFLNPPIAK
                     DDMAKPEPGKESALVDVLANDFDVDSDPGTLKIAQVLGGQGTIVQTPEGPRVSVPIKG
                     YGYTVPYVVEDVDGARAMALVHVPTGSRGKPYVPEGSLITMDTDSTKSVGINDHVKSP
                     AGRTLGITAAGTLSASPSGSLSVTEDGPNGLTLTSSNGYVGPGAVTLEVTDQSAENQA
                     EVNTAYVTIPVQVGPKVPLLRCPTTSITLYAGGLGRELDIPTLCRAWLPQGMTLDDVE
                     FTASWSREPSGVDLQLGGTGKRTAALSASAGAPSSEGVLTIGVKGGQTKGEVTVKVIG
                     VTTTDGAKVETPMPPPRLRPMSIAGLKEGESQTVDVAAYLDSPLAKPACAVEAASVTA
                     GQNVTVSTSGCKVTVTAGKAPSLTATISVLATDGPGRTANGVIDVTLLGRPSAPTAVT
                     AQPDREAGGRARVSWVAPTYDGGTPIRTYTVKWTGGSTGQLACTASPCTIEGLTNGKD
                     YTFTVTATNAVGEGDPSPVSNVARPDTKPGPVGGVAMAARGDGQLTVSWTAPDNKGSQ
                     ISKYTVRLVAADGSSAKTVDVAAPAMSAVVGGLNNEVQYNVSVQAWNEVGAGPFGPAV
                     TMQSAGTPPAVAAPRLTPGGLGPTADLTTVDIAWTATRPNGPPLTGYTVYKSTGGGAF
                     AALATVSANTFTLRDQVSVNGSQYRYVVTATNGAGIESPKANVSSFTAIGQPATPTVA
                     ASTPNPDKTATLVVSLGNPHSTGYASVRWSSSAGGSGTVGCSASNCPAGGQLTIRTGQ
                     LSTVNQTFTVVANNGSQDSASASAQAHPFGPTPTPTDNGSSASGQNLNFRWNLPTDGR
                     PITAVEVQGDFTPSWGSAVTSFSRTGAWDTPYTIRVRAQSAAGWSNWSNNITIRTGLP
                     TPIINLSHGGKISNRLDQGCDPVSPCYYPRVSISNFLGQANCSYFANSVSAGYNDVSD
                     SWSMADGGTWTDTNYFGYITYPQVRVTCNSAKQSLTRTFNWQNP"
     gene            complement(11596..14013)
                     /locus_tag="PKU75_07570"
     CDS             complement(11596..14013)
                     /locus_tag="PKU75_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPF80771.1"
                     /translation="MTGVRIDPRATSTGLPGAGGAAGSRLRALAAGIAGRHALVDLAF
                     AATLVGIALVGFRTGFLGWQWVLAAVAGLVLGLAVAWLGAVRRWPVLLTALVLVAVYF
                     LLGGSLAVRDKLIAGALPSLSTLVDLATTSVTGWKRWLTLLPPVDARGPLLALPWLTG
                     LVGGALTFGVARRWRSAPVVVVAPLVLLVGTIAMGTMQPAAKLVQGVLFALVLILWVV
                     VRAARARASMQNGAGSTARALTGVGLLVVAALAATVVAPHLPGARESDVRHVVRTQLV
                     PPYDVAQFASPLAGFRRYTEPNAAALFDKTILTVKGLPEGTPVRFASLDKYDGLVWGA
                     ADRTSEGVPFQQVGSRIAARTTGTEVTATVSVPEDGYRGAWLPTVGSPTAISFTGARA
                     QALADQLWLNTGTETAVVPAGLAGGESYTMSAILPRTAPLDALPEELPVATGASAPTD
                     TAFLDAKVDAWTSTASGAWQKFAAAAKYMSSNGAYTDGGTPNSIEKVYLPGHGLARLS
                     RFVGSTQLAGNDEQYAAALALVGQRLGIPTRVVMGAIPQAGGEVQGKDVHAWVEVQKE
                     DGEWVGLLPTTFLPDRNKKPNEQQLRTEEQKVGAQVPPPAGTNPPSVLQGPDQAQNAT
                     DLNKRKKNPFDPSSWPLWLQVLVLALLLPLLVLTAIYGLIRGLKARRRRRHALRGPPT
                     ARVAWVWRDLVTDARTLGVAIPTGRSTRLEQAAAFPAPAPAAAVAAAANAQVFGPDEP
                     TDEGPIALLAQAAAVRAELRAGVSRWRRFRSDVSLRPFFDRRTPWSWPRLPKPTLTRP
                     KKAVTAS"
     gene            complement(14010..15674)
                     /locus_tag="PKU75_07575"
     CDS             complement(14010..15674)
                     /locus_tag="PKU75_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPF80772.1"
                     /translation="MTDQFDDDAGQTTRRGDLARRRGRRQGDGMPSAPPPPPAPGGAV
                     AREEAPGPGTPAEGATVSRREVKAARGKAPKPPRGPRPPRRSLLRRGGAASTGTPTPT
                     DSFPSGSAPMARPQTSSRSTRLRELPQITLPRVAVPTAITQTGARVLDITSTRLQPVL
                     LQTAPVWRPVAAFLRWISPLGWTVFGLGVLAWFVAARWGWTELAMMAVACLVLFGLCL
                     LLAIGRSQVEITTDVDPTRVVVGEPATGRIAVRNTGSRGMLPLLVELPVGATAARFVL
                     PTLGSGKVHEELFVVPTHRRGVIAVGPATTVHGDPLGLVRRTVAWTDRTELFVHPLTT
                     PLDPLGAGLLRDLEGQTTQDLSMSDLAFHALREYQPGDDRRYIHWRSSAKHGRLLVRQ
                     FLDTRRSHLSIVVDTTPDSYTGAEDAVELAISAAASLAVRSIMDEQDTTVVVGDQVAS
                     RSTAPLTLDAMARAEVAPVDLFALAGEASSLAPDASVGILVTGPHRPFIQVQRALGQF
                     EVEVIKVAIIVDPDEGVGLRRLGNVTLLTIRELADLRRVLFSGALS"
     gene            complement(15677..>16164)
                     /locus_tag="PKU75_07580"
     CDS             complement(15677..>16164)
                     /locus_tag="PKU75_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPF80773.1"
                     /translation="TYRLPEAQLDRFLMKTSLGYPDHDATVRVLRDAKTRDRTKALSP
                     VVTASVITDMAALADEVHVDDAILDYLSRIAEETRRHVSVRLGLSVRGCLAFIRCAKT
                     WAISQGRNHVIPDDIKLLAQPVLNHRLLVTPEAQFAGITVEQVITQILGEVAPPTERV
                     A"
BASE COUNT         2172 a         5473 c         6097 g         2322 t
ORIGIN      
        1 gcctccgtca cgggcagtcg aaggtcccgc tcgaccgact gcaggttgcg gacgaactcg
       61 gccatggtca gcgggcgatg ctgcgggttc ttggccagcg cctgccggag cacccgttcg
      121 aaggtctccg ggacgtcggg acgaccggtg gccgggggcg gcaggtcacg gatccgggcc
      181 atcgtcgcca tgagggagtt gtcgcccgag ggcaggtcga agggtggtct gccgacgagg
      241 aggtgccaca gggtggcagc caaggagtac acatcggagg cggggctgcc attggtcgac
      301 gtgtagagca tctccggcgg tgaccagggg atcgagacgc cgacatcgct ggacgccgtg
      361 tcatcgcccg aaatctggtg tgccacacca aaatccgtga ggccgggggc accccacggc
      421 gtcttgagga tgttggcggg cttgatgtcg cggtggagca gcccggcccg gtgcgcagtc
      481 tccaccgccg acccgatccg gacacccgtc gagaggacct ccgcgacgct caaccgctcg
      541 cgctcgagtc gctgctcgta ggtgtcgtcg gcgcagtact gcatcgagat gtagggacgg
      601 ccgtcgctgg tctggccgga ggcgtagaca cgcacgatgt tcgggtgctc gagccgagcc
      661 atcgtgttgg cctcggccag gaactgggca acggtgcccg agccgacgtc ggcttcccgg
      721 atcaccttca ccgcaacccg acggccgagg gcgatctcct catagaggtg gacgtcaccg
      781 tagccgccag catcactgat gagccggagg tgccggtgac ccgcgatgtc gggcccggtc
      841 gcgggcatca ggtcgcctcg aaggtcatct cgaagacctc ggcgaggatg atcctcgtgc
      901 ccggggcgat cgtcaccgga gaatcgggtg ccaggcgctg ccggcgtcca tccggggtga
      961 ccacctcggt gccattggtc gaccccaggt cggtcaggca cacgtcccag aagttgagcg
     1021 tcaccgtggc gtgctggccc gacacctcga accgcggatc gttgagcacc acgaggcgcg
     1081 ggagcggcgg cgtcacgccg tccgggacct gggggtcgcg accgagcacg aggtcggagt
     1141 ccagtggcac cgggtcaccg gtcgggagga cgagacggcc aaggaccggc cgcgggatct
     1201 cccgcggcac cgggtccggc ggcatggcga cgccacacac gcggcacacg gcggcgtgcg
     1261 cttggctggg atgtccctgc gggcagtggg cggcccacac cgtgggtccc gccgggaccg
     1321 tgggtggttg ctggagggca gcacggttca ccgtgcgttt gatgacctgt tccggtgcga
     1381 ccgcaggcgc gggagccggc cgttccggca caggggcgct ctcctggggc ggctgaggca
     1441 gcgggaccgg cgagcctggc gcgattgacg aggggccggt cgctgcgccg ggcccacctc
     1501 cgaagtcggg gattgcgtcg atgaagccac cgccaccggt cgacgcgggg gacggggccg
     1561 aagccgctgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggctcgacc
     1681 acgggggccg gctcgaccac cgagggggcc gcaggcgcca gcaagggctc actcacggga
     1741 gctccctgca ccgccggggc ctcctcctgg gggctcggtg gcgaaccgaa gaggtgggca
     1801 tagggattcg ggcccggcag gtcatcgtgg gcgacctgcc attcgccacc agggaccggc
     1861 gggtgcaccg gcatgggtgt ctcgcccggg tcgacgtcct cgacctccgc cgtctccgct
     1921 tcgtggagcg gctccgcttc gtggaccggc tccgctctct cgactggctc cgttccctgg
     1981 accggctccg cttcatcgac cggcccagcc accaccgccg ccagtccgtg ctcggccacc
     2041 gcgcctccac gggagatgcg cacggcgctc gcggggagca ccccggcaac caagggcacg
     2101 ccggcctcgg gggaagcatc atcgacctgc acctggcacc acacgatccc ctcgtggcgc
     2161 tcgtcggcga ggatgccggt cgcggtcacg tggaacggac cggttgcgtc gactccgcgc
     2221 gccgtcgccc gtccgcggac gacgacccga gccccgccgt ccgatcccac ctcgaccagt
     2281 ccgaagtcag gcgcgtccgc cagaccgtgg ctgaggaagg tccccaccac gaggtcggcg
     2341 ccctggccct cgtcgagaag cgtccacgtg ctcagggcca cgggatcggt cagtggacgg
     2401 tcgatgagga cgacgaggga gcccttggca caggccgcct tcgctccagg agcaaaggtc
     2461 agggacgttt cgccggtcac tgggccacct cccggatgac gaggtctcga ggcagggtgg
     2521 tgcgggcccg ctcgggccgg agcgccggtg cgtccgtcac atcggtcacg aggacgacgg
     2581 tgatgttgtc gtgtcctccc gcggcctcgg cggcggcgac cagctcttcg gccagccggt
     2641 ccaggggcgg gtcccccgag cacagcccgg cgatgtcggc atcgctgacc tccgagggca
     2701 agccgtcgga gcagacgagc agggtcccac cgtgaccggc cggcagtgtc acgatgtcga
     2761 cctccggggg tggcacctgg ccgatggccc gggtgatcac gttgcggtcc ggatggaccc
     2821 tggcctcgtc ccgggagatg agaccgcgat caaccatctc ctggacctcg gagtggtcga
     2881 ccgtcacctg ccgaagcgaa ccggaggtga gcaggtagca ccgagagtcg ccgacgttga
     2941 acaccaacca gtcgtcacgt cgccggatcg caccgcacag ggtcgtgccc agaccggcga
     3001 cctcggggtt ctcggccgag tgcccgagga cggctgcatt ggcccgccgc accacctgcc
     3061 ggacctcgtc ctcgctcacg tcctcgcggt ccaggctccg aaccgtatcg atcgtcaggg
     3121 tgctggcgac gtcaccgcac cggtgcccac ccatgccgtc cgcgacgacg aagagctgct
     3181 cccccgcgta ccaggcgtcc tcgttgagtg tccgcacccg cccgacgctg ctcctgccga
     3241 tcgacacgag ggacatcact cacccgcccc gcacggcgtc gaggtagtgc ctcgccgcct
     3301 gaccgaagga ctccttcggc gctgcgatgg ccaccgagct gacgatctgg tcgtggaagg
     3361 cacggttata gcccgacttg tcggtgaaga aggagatgta gcccaggtag cagggcagcg
     3421 ccatcagccc gagcccgatg taccggccga gggcacggcc gaccccgatc ggctggtggc
     3481 tgccgaggtc aaccacggag acgcccatga tgcgcttccc gatggtggcc ccgccgcggc
     3541 cgatgcccag ggcgtagtac cccaggccga tgagcatgaa cacgagctgg ccgagcagtc
     3601 cgaggacgtt cgacacctgg gagagcaccg tgatcacgat ggccaggacc ataaagacga
     3661 cgaacaggaa gacggagtcg acgagattgg ccaggaaccg cgtgccgaga tcgacggggg
     3721 cgacggacga ggtggggctt ccggccgatg gcccggttgt cgccgacggc gtggcgacgc
     3781 cggccggcgg gaacggcgac tccggtgcga tgggcgcggg ctgagcgacg ggcagctggc
     3841 tcctctggac ggttcgcccg ggcgtgggga tgccgggcgc ggggacctcg ggcggtgtca
     3901 ccgcaccggt gaggtcagcc gccggtgcag cgggaccact ggcccagacc tgctgcccgc
     3961 acctgggaca ccgcgttgcc cggctgtcca gctcgagggc acacatcgaa caggtctgcc
     4021 cggcggatcc cgcgtccatc tgtccctcgc tgctcacgac gaagtggacg tgtcggtcca
     4081 cgcccgttac gcacggtagg cacgacgggc tcgcaagacg agagttctgg acccgttgtc
     4141 cccgcaaatg gggacaaccg ggcttcgcct cagccgttgg tgaggtagac cgtcgcgccc
     4201 gtgtcggcga cgcacgtcgt ggggagacga ccggtgcacg tcagcgtgta gtccttgccc
     4261 gtcaccggcg aggtcgcctg cacggtcacc gaacccccat cggtcatgcc cgtcgcgcgg
     4321 taggccgccg cgacgttctg agcgaaggga caggtcgtgt tctggtccgc gccgaagacc
     4381 gcggcggtct ccccgctgcc ggtcactccg caggcgaccg cgcccgccgg caccggtccg
     4441 gccgctgcct tcgagggcga ggtcgagggg gtctggcccg gcgaggcggg cggggacgtc
     4501 tcgggggtcg cgctcgtccc ctccggcgtg ccgcgcgggc tcgtcgtcgt ctgagcgctc
     4561 gggctgctcg acgcgtcgcc ggtgtccgac acccgcacga tcgaccagag cgcgaacccc
     4621 agcgtgagcg cgacgaccag gccggccgcg acgagcaacg cggtcgcccc ggaccggcgg
     4681 ggcggctcgt cgtcccacac cggctcgccc gccggactgc cctggccgta tgccgcgccc
     4741 gcaccggccg cgctcgccgc accgagcgcc ggggtgctcg gggccggctc agttccgacg
     4801 ggtagcgcct cggtcggcgc gaccgcggga ggcgccggat ccgtgggaat cagctccgcg
     4861 gcatacggct ctggcgtata cggctcttcg gcatagggct ccgaggcgta ctgctcctcg
     4921 gccaccgact ccccggccac tgactccccg tccggggcac cttcgggacg cggtcgttcg
     4981 acggtgtccc tcaggtcgtc cgaaccgaag agctcggacg tcagggactc acgctccgcg
     5041 ggctgctccg gtgccggtgc ggcgagggag gtgccgcagt gagggcagaa ggcagcccag
     5101 atcgccaccg gctcctggca cgacggacac tgcacgcgct cggttcctct ccctcgggac
     5161 gacaggacaa cctctccagt ctcgcccgcg gtgacgccga gggcaatccg ggtcaccgcg
     5221 ggcgggctgg acgggccgcg tcctaggggt tctgccagtt gaacgtcctg gtcagcgact
     5281 gctttgcact gttgcacgtc acccgcacct gcgggtacgt gatgtagccg aagtagttgg
     5341 tgtcggtcca cgtcccacca tcggccatcg accacgagtc ggagacgtcg ttgtacccgg
     5401 cagacaccga gttggcgaag tagctgcagt tcgcctggcc gaggaagttg ctgatcgaca
     5461 ccctcgggta gtagcacggc gacaccgggt cacacccctg gtcgagacgg ttggagatct
     5521 tcccgccgtg gctgaggttg atgatgggcg tgggcagccc cgtccggatg gtgatgttgt
     5581 tcgaccagtt gctccagccc gccgcggact gggcccgcac gcggatggtg tacggcgtgt
     5641 cccaggcccc ggtgcgcgag aagctcgtga ccgcactgcc ccaggacggc gtgaagtcac
     5701 cctggacctc cacagccgtg atcggacggc cgtcggtcgg caggttccag cggaagttga
     5761 ggttctgccc gctggcgctc gagccgttgt ccgtcggcgt cggcgtcggc ccgaacgggt
     5821 gggcctgggc cgaggcgctg gcggagtcct ggctgccgtt gttggccacc acggtgaagg
     5881 tctggttgac cgtgctgagc tggccggtcc ggatcgtcag ctgtccaccc gccgggcagt
     5941 tgctcgcgga gcagcccacc gtgccgctgc cgccggcgga ggaactccac cgcaccgagg
     6001 cgtagcccgt cgagtgcggg ttgcccaggg acaccacgag ggtggcggtc ttgtcggggt
     6061 tcggtgtcga ggccgccacg gtcggcgtgg ccggctggcc gatcgcagtg aacgaggaca
     6121 cgttggcctt cggggactcg atcccggcac cattcgtggc ggtcacgacg tagcggtact
     6181 ggctgccgtt gacactcacc tggtcacgca gcgtgaaggt gttggccgac accgtcgcca
     6241 gcgcggcgaa ggcgccacca ccggtgctct tgtagaccgt gtaccccgtg agcggcggcc
     6301 cgttcgggcg ggtggccgtc caggcgatgt cgacggtcgt gaggtcggcg gtcgggccga
     6361 gcccgcccgg ggtgagccgg ggcgcggcga ccgccggcgg cgtgcccgcc gactgcatcg
     6421 tcaccgcagg cccgaaggga ccggcgccga cctcgttcca cgcctggacc gagacgttgt
     6481 actggacctc gttgttgagc cccccgacga cggcgctcat ggccggggcc gcgacgtcga
     6541 cggtcttcgc cgaggacccg tcggcggcca cgaggcggac ggtgtacttg ctgatctggc
     6601 tgcccttgtt gtccggggcg gtccacgaga ccgtgagctg gccgtcaccg cgtgcggcca
     6661 tggcgacccc gccgactggt ccgggcttgg tgtcggggcg ggccacattg ctcaccgggc
     6721 tggggtcgcc ctcgccgacg gcattggtcg ccgtgacggt gaaggtgtag tccttgccgt
     6781 tggtcaggcc ctcgatcgtg cacggtgagg ccgtgcaggc cagctgcccg gtcgagcccc
     6841 cggtccactt cacggtgtag gtgcggatgg gcgtgccgcc gtcgtaggtc ggcgccaccc
     6901 acgagacgcg ggcgcggccc ccggcctcac ggtccggctg ggccgtgacc gccgtcggtg
     6961 ccgagggccg accgaggagg gtgacgtcga tgacgccgtt ggccgtgcgg cccgggccgt
     7021 cggtcgccag gacgctgatc gtggcggtga gggagggtgc cttgccggcg gtgacggtga
     7081 ccttgcaccc actcgtgctc accgtgacgt tctgcccggc ggtcaccgag gcggcctcca
     7141 ccgcgcaggc cggcttggcc aggggactgt cgaggtaggc cgcgacatcg acggtctggg
     7201 actcaccttc cttgagcccg gcgatgctca tcgggcgcag gcgaggcggg ggcatcggcg
     7261 tctcgacctt ggcaccatcg gtggtcgtga ccccgatgac cttgacggtg acctcaccct
     7321 tggtctggcc gcccttgacg ccgatcgtga ggacaccctc gctgctcggc gccccggccg
     7381 aggccgacag ggccgccgtc cgcttgccgg tgccgccgag ctgcaggtcg accccgctcg
     7441 gctccctgct ccacgaggcg gtgaactcga cgtcgtcgag ggtcatgccc tggggcagcc
     7501 aggcccggca cagcgtgggg atgtcgagct cgcgacccag cccaccggca tacagggtga
     7561 tcgaggtggt cgggcaccgc agcagcggga ctttcggccc gacctggacg gggatggtga
     7621 cataggcggt gttgacctcg gcctggttct cggcgctctg gtcggtgacc tcgagggtga
     7681 ccgcgccggg gccgacgtag ccattgctcg acgtcagggt caggccgttg gggccgtcct
     7741 cggtgacgct gaggctgccc gacggcgagg cggacagggt gccggccgcg gtgatgccca
     7801 gggtgcgtcc ggccgggctc ttgacgtggt cgttgatgcc gacgctcttg gtggagtcgg
     7861 tgtccatcgt gatgaggctg ccctcgggga cgtacggctt gccccgcgag ccggtgggca
     7921 cgtggacgag cgccatcgcg cgggccccgt cgacgtcctc gacgacatag gggacggtgt
     7981 agccgtagcc cttgatcggc acgctcaccc gcgggccctc cggcgtctgg acgatggtgc
     8041 cctgcccgcc gaggacctgg gcgatcttga gggtgcccgg gtcggagtcg acgtcgaagt
     8101 cgttggcgag cacgtcgacg agggcgctct ccttgcccgg ctccggcttg gccatgtcgt
     8161 ccttggcgat cggcgggttc aggaagtcct tgcggccacg cacgaggatg ctcgcgcggg
     8221 acgggtcgaa catgccgttg tcgatcgtgt agaccgtgtg gtgcagcggg gcggtctcct
     8281 ccgggacgac cgtggagacc atccgcgtct cctcgtcgac gcgccagccc tcggtgtcgc
     8341 cgagggtgag gtcgacgacg tcaccacgcg cgatgaggtc gttctgcagg accgggatgg
     8401 tcacggtctt gccgggggcg gcggtcaccg tgtcgtcgac cgcgaccggc ggctgcgggt
     8461 cgcccggtgc caccacgccg acccggacga aggcgcggct cagctcaccg aagcggtccg
     8521 cgacctcgta gtcgatgacc tcggtgccgg ccgccgtcgg gtaggcctcg tagcggatcg
     8581 tgtccggtcc gacggagacg atccggccgt acctcaggct gacggcgcca ccgtcccggc
     8641 ccacgatccc gcggatgctc accgtgtcgc cgtcggggtc gactccgctg gtgggcaccg
     8701 tgatggtcag cgggtcaccg gcggtgaccg acgtggtgaa ggagcgcgcg acaggggcct
     8761 ggttgggccg ggccgtcgtg ggctgcggcg tcaccgcgac ggagacccga ccggtctggg
     8821 cgcgctgcgg gtcgccgtcg gggtaggcgg cgtactcgat ggtcacgtgg tcgtcggcgg
     8881 tgagcccgct cgcggtgggg acgaaccgga tgaggttgtc ggaggcgaag gcggtctgct
     8941 cccgtccgtc gaggaccttg accgacagcg ggtccaggcg cagcgggatg ccgtcggcca
     9001 tggtgtcgtt gtcgagcacc gggatcgaga ccgcgtcgcc ctcgcggacg atggccttgt
     9061 cgtcgcgcac gaccggggtg atcggggtca ccgccttctg gacgaccgag atctggccct
     9121 tcgcggtccg ggtcccgtca ctcacggagt agttgaccgt cccggggcgc gcgaaaccgt
     9181 ccccgccggt cgccggctcc agggagcgca gccgcaccca gcggccctgg tagatcgagg
     9241 cctcgatcca cgcgctgctg gactccaccg acacgctctg ggtgaccatg acgtcggccc
     9301 gcgggctgta gtcattggcc aggacgtcga ccatgctcga cagctggccg cgcagggtgc
     9361 cggcatcgga gaccgcgatc gggggtgcgt tcgggtccgg gtccccgatg acgtcgatcc
     9421 ggatccggcc gggggcgacc ccgctgccga cctgcgcggc atacgagagg atgaaggtgc
     9481 ccgccgccgc cggggtgatg gtgaccaccc cggtgtcctt gttggtgtcg acggtgaggt
     9541 tgccgaccgg ctgcacgtcc ccggccagcc gcatctcggc ctcggggtcg cccgggtcgg
     9601 ccccggggat gtcattggac agcggctcga cctggatcgg cttgccgacc acgccgcgga
     9661 tcacgtccgg ctgcgtgacc gggttgcgcg ggcgcgagtc gagcggaccg acgacctcga
     9721 cgtcgaccga cccaggcgtg gtcccgccct gcccgtcgtc gacggtgtac gggacgctga
     9781 cgacccccgg ctccatgccg gagaggtact ccacccgacc ctggccgtcg acgctcgagc
     9841 cggcgtcggc cgccgaggcc gagaccgtgt cggactggtc gtcgcgccag tcaccgatga
     9901 ccggcagcga gatgcgccgc ccggcgagca ccgggtaggc ggacctggcc aggtccttgg
     9961 atccctcacg cagccgcggc ggggtgttga cctcgtcacc gacgatggcg acggtcacgg
    10021 tcgtcgccgc ggacttggcc ttgccgttgc ccaccatgta ggtgaacgtg aaggagtcct
    10081 tgtgaccggc gggcacggtg acgtcgacgg tctggccgtc ggccgcgacc gaggtcccca
    10141 ccttctcgtc gctgggcttg gacaggtccg tggggtcgat cgccaggacc gagccggcga
    10201 catcggtgtc gttgtcgagg acgtgcagct tgctcgtccg gccggcgcgc accgtgaggc
    10261 tgtccggcat cgccgtcggc ggctgctcga ggacggcgtc gtcgacgagg ttcttgtcct
    10321 tcttgtcgtt gtcgttctcg gtctgtggcg gcgggatgag cgagtcccag ttgtcgatct
    10381 tcttctgctg gtcgcggtcg acgtcccacg cggccccgct gtcgaggtcg ttgaggacga
    10441 tggcgccgcg gttgacgcgg aacttcacgc ccgagcggat cgccttgtcc gagatgttct
    10501 cgatcgtgcc ggtcgggacc gggttcgcga ccccgcagtt ggcgccgtag aagaccttgt
    10561 tggcctcgtt ccacgcgccg tggacacaac ccttgagcac gaccggagcc gcgacgtgcg
    10621 ggacctcggc gctgcggttg actcgttcct gcacctcgat gccgcccggc gcggggtcgc
    10681 cgtcgaggcg gatcatgcgc acggccgcgg tgccggcgat ggcgaccgcg tccgccgccg
    10741 ggccgggcac ctggaggagg ccgtatgccg cgtggtcacc atcggtggcc aggcccgcct
    10801 tgaccccgtc gggcttcccg gcgctggcga gccggtcggt cgtggggtcg taggcgaccc
    10861 agtccttgcc gagggcgctg acctggatgc cggcgccctt gaggccgatc tcggtcgtca
    10921 ccggcgcggc gaaggcctgg ccgccggggc ggatcgtggt catcgtcgac ttcgcacccg
    10981 acacgacgtg gacggcgccg tcctcgccga cggccagggc cgcattggca cccaccgtcg
    11041 cgatcggctt gctcgtggtg cccagcttgg acaggtcggt gatgccggcg cggtcgtcga
    11101 cgcgctgggc ccacaccttg ccggacttct cctcgacgag ggcgacggtg ccgccgcgca
    11161 ggtccacgac gctcgtgggt gccactcccg cggtcggggc cgcctgggcg ggcacgatcg
    11221 cggtggcgga cgtcgtcgtc gacgtcttgg ggtcgagggc ctggagctgc tgggtcgccc
    11281 ggctccacgt gaggatcgct gccccgtcct ggaggacgtc gatcgacgag cccggctcga
    11341 cgtcggccga gacgccggcg tcgagctggc cggcctggac gttgaggcgc ccgaacttgg
    11401 ccagggcgtc gctggtgagc cagatgccgc cgtcgccgag gtcggcctcg tgcacggtcg
    11461 agccgcggga ggtggcggcg gccaccccga gggaggtggc cgcgagggcc acgacggtga
    11521 gggaggcgat gcggccgcgc cgggccgacc gggctgctgc cggtgagccg gcgggtgctc
    11581 gtgtgccgaa ggggttcacg acgcggtcac cgctttcttg ggacgcgtga gggtgggctt
    11641 gggcagtcgc ggccacgacc agggggtgcg gcggtcgaag aacggacgca gcgagacgtc
    11701 ggagcggaag cggcgccagc gggagactcc cgcgcgcagc tcggcgcgca cggcggcggc
    11761 ctgggcgagc agggcgatcg gcccctcgtc ggtcggctcg tccggcccga acacctgggc
    11821 attggcggct gcggcgacgg cggccgcggg ggcgggcgcc gggaaggcgg cggcctgctc
    11881 caggcgggtg ctccggccgg tggggatggc caccccaagg gtgcgtgcgt cggtgacgag
    11941 gtcacgccag acccaggcga cgcgcgccgt gggcggaccc cggagggcgt gccggcgacg
    12001 gcgacgggcc ttgagcccgc ggatcaggcc atagatcgcg gtgaggacga ggaggggaag
    12061 caggagcgcg aggacgagga cctgcaacca cagcggccag ctgctggggt cgaaggggtt
    12121 cttcttccgc ttgttcaggt cggtcgcgtt ctgcgcctgg tccggcccct gcaggaccga
    12181 gggcgggttc gtgccggccg gcggcggcac ctgggcaccg accttctgct cctcggtccg
    12241 gagctgctgc tcgttgggct tcttgttgcg gtccggcagg aaggtcgtcg gcagcagccc
    12301 gacccactcc ccgtcctcct tctggacctc gacccaggcg tggacgtcct tgccctgcac
    12361 ctcgccaccg gcctgcggga tggcgcccat gacgacgcgt gtggggatcc ccaggcgctg
    12421 gccgacgagc gcgagcgccg cggcatactg ctcgtcgttg ccggcgagct gggtcgatcc
    12481 gacgaagcgg ctcaggcggg ccaggccgtg gccggggagg tagaccttct cgatcgagtt
    12541 cggggtgccg ccgtcggtgt aggcgccatt ggagctcatg tacttcgccg ctgccgcgaa
    12601 cttctgccag gcgccggacg ccgtgctcgt ccaggcgtcc accttggcgt cgaggaaggc
    12661 ggtgtccgtg ggcgcgctgg ccccggtggc cacgggcagc tcctcgggca gggcgtcgag
    12721 cggggcggtc cgcggcagga tcgcgctcat cgtgtaggac tccccgccgg ccagaccggc
    12781 cgggaccacg gccgtctcgg tgccggtgtt gagccagagc tggtcggcga gggcctgcgc
    12841 gcgggcgccg gtgaaggaga tggcggtcgg cgagcccacg gtcggcaacc acgcgccgcg
    12901 gtagccgtcc tccgggaccg agacggtcgc ggtgacctcg gtgccggtcg tgcgggcggc
    12961 gatccgcgag cccacctgct ggaaggggac accctcgctc gtgcggtcgg ccgcgcccca
    13021 gacgagcccg tcgtacttgt cgaggctggc gaagcggacc ggggtgccct cgggcaggcc
    13081 cttgaccgtg aggatggtct tgtcgaacag ggccgcggcg ttgggttcgg tgtagcgccg
    13141 gaagccggcc aacggcgagg cgaactgcgc gacgtcgtag ggcggcacga gctgggtgcg
    13201 caccacgtgc cggacatcgc tctcccgggc accggggaga tggggtgcga cgacggtggc
    13261 cgcgagggcg gcgacgacga gcagaccgac tccggtcagg gcccgagcgg tgctgcccgc
    13321 gccgttctgc atcgaggcac gcgcccgcgc ggcgcgcacg acgacccaca ggatgagcac
    13381 gagggcgaag aggacgccct ggacgagctt ggccgccggc tgcatcgtcc ccatggcgat
    13441 cgtccccacc agcaggacga gcggggcgac cacgaccacc ggcgcggagc gccagcgccg
    13501 ggcgacgccg aaggtgaggg cgccaccgac gagtccggtg agccagggca gggcgaggag
    13561 cgggccgcgg gcgtccaccg gaggcagcag cgtgagccac cgcttccagc cggtgacgct
    13621 ggtggtcgcc aggtcgacca gggtgctcag gctgggaagg gcaccggcga tgagcttgtc
    13681 ccgcacggcg agcgatccgc cgaggaggaa gtagaccgcg acgaggacga gcgcggtgag
    13741 cagcaccggc caccggcgca cggcaccgag ccaggccacc gccaggccga ggacgagccc
    13801 ggcgacggcc gcgagcaccc actgccaccc caggaagccg gtgcggaagc cgaccagggc
    13861 gatcccgacg agggtggccg cgaacgcgag gtcgacgagg gcgtgccggc cggcgatgcc
    13921 ggcggccagg gcgcgcagcc ggctgcccgc ggcgccgccg gcccccggga ggccggtcga
    13981 tgtcgcccgg gggtcgattc tcactccggt cacgacagcg ctccactgaa gaggacccgc
    14041 cgcaggtcgg cgagctcacg gatggtcagc agggtgacat tgcccagccg ccgcaggccc
    14101 acgccctcgt cggggtcgac gatgatggcg accttgatca cctcgacctc gaactggccc
    14161 agggcgcgct gcacctggat gaagggacgg tgcggcccgg tgacgaggat gccgaccgag
    14221 gcatcgggcg ccagcgagga ggcttcgccc gcgagggcga acaggtcgac cggcgcgacc
    14281 tcggcgcggg ccatcgcgtc gagcgtgagt ggagccgtgc tgcgggaggc gacctggtca
    14341 ccgacgacga ccgtcgtgtc ctgctcgtcc atgatcgacc tcacggcgag cgaggccgcg
    14401 gccgagatgg cgagctcgac ggcgtcctcg gccccggtgt aggagtccgg cgtcgtgtcg
    14461 acgacgatcg acaggtgcga tcgcctcgtg tcgaggaact ggcgcacgag gagccggccg
    14521 tgcttggccg agctgcgcca gtggatgtag cggcggtcgt ccccgggttg gtactcccgc
    14581 agggcgtgga aggccaggtc cgacatcgac aggtcctggg tcgtctgccc ctcgaggtcg
    14641 cgcagcaggc ccgcgccgag tggatccagc ggggtggtca gcgggtggac gaagagctcg
    14701 gtccggtcgg tccaggccac ggtgcgccgg accaggccca gcgggtcgcc gtggacggtc
    14761 gtggccggtc ccacggcgat gacgccacgc cggtgggtgg ggacgacgaa gagctcctcg
    14821 tggaccttgc ccgacccgag ggtcggcagc acgaagcggg ccgcggtcgc gccgaccggg
    14881 agctcgacga gcaggggcag catcccgcgt gagccggtgt tgcggaccgc gatccggccg
    14941 gtcgcgggct ccccgacgac gacccgggtc gggtcgacat cggtggtgat ctcgacctgg
    15001 ctgcgcccga tggcgagcag caggcacaac ccgaagagga cgaggcaggc cacggccatc
    15061 atggccagct cggtccagcc ccagcgcgcc gcgacgaacc aggccagcac acccagcccg
    15121 aagacggtcc accccagggg tgagatccag cgcaggaagg cggcgaccgg tcgccacacc
    15181 ggagccgtct ggagcagcac cggctggagg cgggtgctcg tgatgtcgag gacgcgggcg
    15241 ccggtctggg tgatggccgt cggcaccgcg acccgcggca gcgtgatctg cgggagctca
    15301 cgcaggcgcg tggaccggct cgaggtctgc ggccgggcca tcggtgcgct gccgctgggg
    15361 aaggagtcgg tcggcgtcgg ggtcccggtc gaggccgctc caccgcggcg cagcaaagat
    15421 ctccgcggcg gcctgggccc cctcggtggc ttcggggcct tcccgcgggc ggccttgacc
    15481 tctcgacgac tgacggtggc gccctcggcg ggcgtgcccg ggccgggcgc ctcctcgcgg
    15541 gccaccgcac caccgggagc cggcggcggg ggcggggcgg acggcatacc atcgccctgg
    15601 cgccgaccac ggcggcgggc gaggtcacca cgccgcgtcg tctggccggc gtcgtcgtcg
    15661 aactggtcgg tcatcgtcag gccacacgct ccgtgggcgg cgcgacctca ccgaggatct
    15721 gggtgatgac ctgctcgacc gtgatgccgg cgaactgcgc ctccggggtc acgagcaggc
    15781 ggtggttgag caccggctgc gcgagcagct tgatgtcgtc ggggatcacg tggttgcggc
    15841 cctggctgat cgcccaggtc ttcgcgcagc ggatgaaggc caggcagcca cgcacggaca
    15901 gcccgagccg cacgctgacg tgccgacggg tctcctcggc gatccgcgac aggtagtcca
    15961 ggatcgcgtc gtcgacgtgg acctcgtcgg cgagcgccgc catgtcggtg atgacgctcg
    16021 cggtgacgac cgggctcagc gccttggtgc ggtcacgggt cttggcatcg cgcagcaccc
    16081 ggacggtcgc gtcgtggtcg gggtagccca gcgaggtctt catgaggaag cggtcgagct
    16141 gggcctcggg caggcggtag gtgc
//
LOCUS       DAOPJG010000062        16077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3829_length_16077_cov_8.521533, whole genome shotgun sequence.
ACCESSION   DAOPJG010000062 DAOPJG010000000
VERSION     DAOPJG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 16077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16077
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3829_length_16077_cov_8.521533"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKU75_07585"
     CDS             <1..535
                     /locus_tag="PKU75_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HPF80774.1"
                     /translation="DVLFYVRQKHQDLLTQLAVSIQNYLAIDIVIKNNIELIKGVDRA
                     TTTTISALRTAVIVSQALSNQQLVLDQITALNTTTSGLIERTSEMLKDNSIRIQEQAA
                     SATIGLPQLQRAFQNIYATMDAIDTFKVQALDNMAATIGTLETEVAKSRSYLDRVQRQ
                     DQRVISGSLDIGKTNTF"
     gene            563..1348
                     /locus_tag="PKU75_07590"
     CDS             563..1348
                     /locus_tag="PKU75_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80775.1"
                     /translation="MGIGEWFGFGKKKEPEPIIEVPAAPTSESLAAALDSVEAMARDG
                     KVPPMVMARLKRVTNVVRQTIPRLDNLGAGSASAYNVMATATDYLPGAIGGYLRLPRD
                     WADTRPVDRGKTSLLILIDQLDLLGGTMDKVYDAVNRADAEALVVHGRFLQEKFGTAA
                     GGELGLGADAQVPPQPTSGSYDAGGRLAPPTATPAWPAPDGQSAATGTDWLAPDKDPD
                     TGDVPKVIHPATPAAPLRPDSTTHAGSATAEPGRLTPPTGRDA"
     gene            1352..3136
                     /locus_tag="PKU75_07595"
     CDS             1352..3136
                     /locus_tag="PKU75_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF80776.1"
                     /translation="MTIPADNLRTAVDQLAAAATAAGLDPVAARAEGFAFAAAIAESA
                     PRAALDWAAAAGIDTADAAAANTAFFEAASGGRKWRQAPTAIVSELVATGSPQATAYA
                     AALGEIASAACLLGDPTPRIAGNATLAAASQLSALPVSAQPRGPAAIPPPATGQPPAS
                     PATPPPAAGAPTGPVLGVGNPDAIPNLTELMLDNQRRVQEQLAAIERLRGGPAFPTPG
                     TPPAGGMPTGPGAVPAPPPGPGVIPTYPSQPPAAPAQPGQPPADPAAAQGQPAQEPEP
                     QKSVEELLAELDEMTGLDSVKREIHQQVAILKVEKKRSEAGLKTATITRHLVFVGNPG
                     TGKTTVARMVSGIYRALGLLTKGHLVEVDRSELVAGYLGQTAIKTAEVVTKAIGGVLF
                     IDEAYGLSGDQYGQEAINTLVKEMEDNRNDLVVIVAGYPGPMMEFIAQNPGLASRFKT
                     MIEFPDYTNDELIDIMAGLAAKSDYDVTDEAEAVVREILSRTERNFTFGNGRFVRNLL
                     EEAIGRHAWRLRDDEDVTTEDLRRLLAEDFREAEEHLAEDEPAGPPPADDTAEAEAEM
                     TAEGGPEPDAEGPEPDAEGPDENKESGV"
     gene            3133..4458
                     /locus_tag="PKU75_07600"
     CDS             3133..4458
                     /locus_tag="PKU75_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80777.1"
                     /translation="MTQIASTPTTPSAVGGQPGGAVSTATSGAAERRAQSRRHYFTGT
                     PGRMRLMAAIASALCLAFALAGFTGLRGVQTSVDRASANTEQVVRARAIYADLLAADA
                     AVTNGFLVGGLESAASRKTYDEAMARVAENIATAASAQEADGKALAALNEEVQNYAAN
                     IDRARTYNREGKPVGAQYLRIAGTDLRAQALPIADAVATANEGRAEDAYGIGAPGWLA
                     LGTGLVALGGLLAIAVWLARRTHRYVNLPLTAAILLVGAALGLAAWHVTQVANTLNDA
                     RNGEYKAATALAQARAAAYDAKANESLTLIARGSGQTFEKAYQDSSAKTLDKLQELVN
                     ADPAQDSLRSRFGAYTVKHSAIRKADDGGDWTGAVKLATSDAADSANDTFRQFDQAAS
                     TVLAQEVKSITTAFDRIGGWLLPWVAGGAAALAALLAGRSMSRRIEEYR"
     gene            4464..5447
                     /locus_tag="PKU75_07605"
     CDS             4464..5447
                     /locus_tag="PKU75_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPF80778.1"
                     /translation="MPRPPSPFKAGGVALLAALGLAACAGGDYPDTPLPTSASASSSA
                     TTAAPTTPVSCDNATQTYDPLASVPARSQITDGAMSDIIQRGYLRVGVSADSYLLGAN
                     NPRTGQIEGFDIDVARAIAEAIFGDPNKARLVVITAADRIPALQDGRVDLVARNMTMN
                     CARWQDIAFSSEYYHAGQKILVGIDSKATSLDDLKGQRVCAPTGTTSLAKLQEVNDQV
                     GGISIVTAANHTGCLVKFQQGEADAITGDDTVLAGLAAQDPYAKVPDSEAITDEPYGL
                     GIAKENTYFVQYVNAVLDEMRADGRWTKIYNTWLAPSLGKAPKPPTPVYGR"
     gene            5447..6664
                     /locus_tag="PKU75_07610"
     CDS             5447..6664
                     /locus_tag="PKU75_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80779.1"
                     /translation="MSLDLGMTASPGGTPRAPGRLGVPVTAAEALRYLTDLGLWLDER
                     RKQLDAIDQAALAAPDQTDREALTHDITVSMALWKAISDRYELLRVAFDSGRVGPVQA
                     EQLSTLIWGRLDVAPGQGREATRARMPSGAEGTLAVSLPEACRLSDAMASSLRARLAL
                     ETTGLDVSIRVKAIREGVERIRDQIAMMPLGTAREKAQHELVGTEQRLFDVTERAKRG
                     ADVGGFLGPLEIDVAARERDLIIAASRRHESARDAARARELIADLAARGDAIRSLQAQ
                     CLQSVSPAPKLAVPDVRALGAVPEDPDAVDALLARLALVGKALDQAHETYARALAERD
                     ELTGLVGALDAQVAASPAVSDAERADLAPMRAQLAATLAATPIPLVRARALMAAYQSY
                     VQAVTTNTGRAPR"
     gene            6661..9123
                     /locus_tag="PKU75_07615"
     CDS             6661..9123
                     /locus_tag="PKU75_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF80780.1"
                     /translation="MSELTACRQPGCTGTVADGYCDVCGMPGEPDQIAAAAAPPAPAA
                     ARPAPAAAAPAPPPPVAQPSGVPPSPWRRGQAGAPRGAGPSCRQPGCTGTIVDGYCDV
                     CGTPATPTSTMATQPVGAGESPVEASNSQVSARLDATALGSERARSGGTISTRRVSGH
                     SQRMRTARIGAGVTSVPPVPEIDAAKAVIKNPVVPEDKRFCPKCGEPVGRGRDGQEGR
                     TTGFCANCRTPFDFSPKLKPGDLVAGQYEVAGCLAHGGLGWIYLARDRNVSNRWVVLK
                     GLLNSGDPDALAAALAEQRFLAQVSHPNIVEIYNFVSHEGAGYIVMEYIGGTSLKSLL
                     KQRMRAAGKYDPLPVDQALAYIIEILPAFQYLHDLGLVYCDFKPDNIIQIGDEVRLID
                     LGGVRRLDDQDSAIFGTVGYQAPEVAEHGVSVASDIYTIGRTLVVLTMEFRGYQTKYV
                     KSLPPVEEVAVFEQYDSFYRLLLKACAPSPDDRFVSADEMRVQMLGVLREVVAQDKNA
                     GAATTSAPSVLFAPPAAGDSHLTYADLPALHPDSSDQQIGWLATIAAQEPAQRLRALA
                     SAPGATPEVFLAQARAALELGQWEVLDGVVQRMLAEDPWEWRAVWMQGLAALARKSPT
                     EAQSAFNAVYGQVPGELAPKFGLAIACALSGNDEIAERLYTICARTDAAYVPQAAFAL
                     ARIRGSRGDTDGVQQALTLVPPNSSAYPLARQHWAEHLAAQPRDTADLTLALQTADDA
                     GFSRRRRAELGVDIYGKALARVEESGPKPGERIGDFPVTADGMRSGLERALRLLADVT
                     DNPLERMRLIDKANDIRPWSLV"
     gene            9528..10940
                     /locus_tag="PKU75_07620"
     CDS             9528..10940
                     /locus_tag="PKU75_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPF80781.1"
                     /translation="MSEHNVDPASLPQPEAAATPAPPAEGEESPLDIGWTGVVPGAAS
                     SYDATPVAVRCGTCGEGVYTDGYCDQCGAKEPNPRDHLEENVAVWVGGVSDIGRRHSR
                     NEDALALAAQDDPVSWAVLVVCDGVSNTTDSDIASLAAARAARTVLEQPLSSGMGVAA
                     AAQAAATKRLGEAVAAANGAVIGKTKQGDEHSPSCTFTGAIVDGRTAYVANVGDSRIY
                     WLPDSGGGEQLSIDDSVAAEQIASGIERKVAETGPMAHSITRWLGIDAPDDLTPHTRT
                     IQLSEPGWLMLCTDGLWNYCSEAGALHDKIVEFQANGTPAQPLPLARALVGFANNAGG
                     ADNITVALARIPADGEDVAAAGAVLPAPAPEATAEVPPVPGETSDATAPDPAGVTDAS
                     TQAPGIPAEESGTPAGPLEAPAEAPAETPAQAPGIPGEQPSIPAEQTAAGAAAPPTGE
                     TQDTESAGDLAGSAHTEKGQ"
     gene            10940..12217
                     /locus_tag="PKU75_07625"
     CDS             10940..12217
                     /locus_tag="PKU75_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF80782.1"
                     /translation="MAQFTAEAFQNQYLPDGATDVHAIVRVDASGATTAAAMSGQGAA
                     EVIIVDTSGSMGEKGIREGAVAATAALQEIADGTNFAVIAGNHLAWQVYPSAPVPGTA
                     IMDPANRAAAMNAVRGFRADGGTAMGQWLLAARQVFLTVPDTYRKHAILLTDGANEHE
                     TPDQLTWAIQSCTGAFQADCRGVGDRWQVDEVRRIASALLGTVDLIPQPEQMAEEFKT
                     LMRASMSRGVASAELRVWAPQGAQVLFVKQVSPNLEDLTTRRREVNPLTGAYPTGAWS
                     DESRDYHVAVRLPAKSVGQEQLAARVQIAIGEEVQAQALVKAMWSNDDALTARINPQV
                     AQYTGQAELAQAIQDGLAAKAAGRDDEATSKLGRAVQLANEAGNAEMTTRLKKVVDID
                     NAETGTVRLKKQVEKLDEMALDTASTKTQRVRK"
     gene            12214..13392
                     /locus_tag="PKU75_07630"
     CDS             12214..13392
                     /locus_tag="PKU75_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPF80783.1"
                     /translation="MSAVCPNGHDTVSTDYCDVCGSPVAAGASSEAPAAAAPAAAASG
                     NSLDLPPVGGDSGPKDCPNCGTPNASSSLFCEACGYDFTTGALPAAAPASLEGVSAAG
                     LESPAPPVREPAGAGLAPESEQPPAFAPDVAAAETESEAEGTPDGSGELAGLNAAEEL
                     DADGLSVSAEGEPGDGEPGPGEPGDGGIPAPDAPESPEAPDGSDAPEGAGAFEGAGAS
                     EGAGAPSEPAPVSGEAEAGAGEQPPVLGGVDPNVPPRIPFQWVAEVWIDPDWYADQQA
                     EEPLPSAGLPVVVPLRIASALIGRASASRNIVPDIDCSADPGVSRRHAQLTTDGSRWF
                     VEDLQSSNGTFVGPASGPLPDDPIAVGPKTEVGDNRIYVGAWTRIVVRRATDEEIAIG
                     "
     gene            13389..14159
                     /locus_tag="PKU75_07635"
     CDS             13389..14159
                     /locus_tag="PKU75_07635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HPF80784.1"
                     /translation="MRPLTFLTIEHEDQCPPSWLGEWWEERGVTLRRVRAHVHPDGVA
                     AEMIPADVGDADALVVLGGEAGANDDADYPWLPATRELIARSVEGGIPFLGVCLGHQL
                     ATVALGGEVRRNPAGMATGLTPVALTPAGATDPLLGGCDGLRVVQWNSDIATRLPERA
                     VQLATAPDGTPQAVRFAPWAYGVQFHPEATPEQVDSWWVDKPSAAEARERAAAASAAI
                     HAARDELRTAWRPFAARFVDLARDFRAGRLDAPVTTGD"
     gene            14170..>16077
                     /locus_tag="PKU75_07640"
     CDS             14170..>16077
                     /locus_tag="PKU75_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPF80785.1"
                     /translation="MPPTDLGRRPAGGMPRTAAVASLAAVLTYAVGSGIASALQPTGY
                     AADQQSVTDLLADGVPFRWVAVGTFVLSGLMVVLAAIALPVARSRRGVLAVGGAAVTV
                     LGLLPRDSMAVVEAPLLGCALVALLSLACWPVAGRRAREGDRIRAGVLLALVAGLGLA
                     AVGDLGYGAYERVLAVALLGHVALAALHAWWVAGHRLGSRPVRMAVAAVVLGAAGMVG
                     GIVTTVVMPAHVSMQYVDIRLALSPSPSDLGRVVVPTVLGDLEAGFAGIAPGVRAFPQ
                     VKADVVTSIGSGDASLETLRPSPAELDRAIRDAAIGLGFRVGVGALVVIGLAIALRAT
                     TMRRGPGLGTALVVVAALVLALGTIAGTVAQTYRSGTPREFKASGLLGTIQQNSRVFD
                     DVAVRSEQVAPYLRNVVALSEALRERYEPQPIGGESVLRLLLVSDIHAGNQYPLLKNL
                     IETEQIDAVIDCGDLVNFGTVTEGEFAGVFEGIGSLGVPYVFVRGNHDATSAADDAVL
                     RRLDRIPNVVLLQPDSTRYEEFTIAGVRIAGFNDPRWFGDDGHGSADKQAPARERWLA
                     SFAGREVPDVVVSHEPWAVQGITGAGVLLNGHMHTAFREGNRVQAGTFTGGGPFSHYI
                     TGEAGEELRGQP"
BASE COUNT         2505 a         5872 c         5560 g         2140 t
ORIGIN      
        1 ggacgtgctc ttctacgtcc gccagaagca ccaggacctg ctgacccagc tggccgtcag
       61 catccagaac tacctggcca tcgacatcgt catcaagaac aacatcgaac tcatcaaggg
      121 cgtcgaccgg gcgaccacga cgacgatcag cgccctgcgc accgctgtca tcgtcagcca
      181 ggccctctcc aaccagcagc tcgtcctcga ccagatcacc gccctcaaca ccacgacgag
      241 cggcctgatc gagcgcacct ccgagatgct caaggacaac agcatccgca tccaggagca
      301 ggccgccagc gccaccatcg gcctgcccca gctgcagcgg gcgttccaga acatctacgc
      361 cacgatggac gcgatcgaca ccttcaaggt gcaggcgctg gacaacatgg ccgccacgat
      421 cggcaccctc gagaccgagg ttgccaagtc ccgcagctat ctcgaccggg tgcagcgcca
      481 ggatcagcgg gtcatctccg gcagcctcga catcggcaag accaacacct tctaagacac
      541 gccgcgacga gctgatcgag acatgggtat cggggaatgg ttcggtttcg ggaagaagaa
      601 ggagcccgag ccgatcatcg aggtgcccgc ggctccgacg agtgaatccc tcgctgccgc
      661 actggattcg gtcgaggcga tggcccgcga cggcaaggtg ccgccgatgg tcatggcacg
      721 actgaagcgc gtgacgaatg tcgtgcgcca gaccatcccc cgcctggaca acctcggcgc
      781 cgggagcgcc tcggcctaca acgtcatggc cacagcgacc gactatctgc cgggggcgat
      841 cgggggctat ctgcgcctgc cgcgcgactg ggcggacacc cgccccgtgg accgcggcaa
      901 gacctcgctg ctcatcctca tcgaccagct cgacctgctg ggcgggacga tggacaaggt
      961 gtacgacgcg gtcaaccgcg ccgacgccga ggccctcgtc gtgcacggac gattcctgca
     1021 ggagaagttc gggaccgccg ccggcggcga actggggctg ggcgccgacg cgcaggtccc
     1081 gccgcagccg accagcggat cgtatgacgc gggcgggcgg ctggccccgc cgaccgcaac
     1141 cccggcgtgg ccggccccgg acgggcaatc ggctgccacc ggcacggatt ggcttgcgcc
     1201 ggacaaggat ccggacaccg gggatgtccc caaggtgatc caccccgcga cgcctgccgc
     1261 accgcttcgc ccagactcga ccacgcacgc cggctcggcc accgccgagc cgggccgcct
     1321 gacgcccccc acgggaaggg acgcctgaca catgaccatt ccggccgaca acctgcgcac
     1381 cgccgtcgac caactggccg ccgccgccac cgccgccggg ctcgacccgg ttgccgcccg
     1441 cgccgaaggc ttcgcgttcg ccgctgccat cgccgagtcc gcgccccgtg ccgccctgga
     1501 ctgggccgcc gccgcgggca tcgacacggc cgatgcggct gcggccaaca cggccttctt
     1561 cgaggcggcg tccggcgggc gcaagtggcg ccaggcgccg acggcgatcg tcagcgaact
     1621 ggtggcgacg ggctcgccgc aggccacggc atacgccgcg gctctcggcg aaatcgcctc
     1681 tgccgcatgc ctcctcggcg acccgacgcc ccggatcgcc ggcaacgcaa cgctggcagc
     1741 cgcgtctcag ctgagcgcgc tgcccgtcag tgcccagccg cgcggaccgg ccgccattcc
     1801 gccgcccgcc acggggcaac ccccggccag cccggccaca ccccctccgg ccgctggcgc
     1861 gccgacgggt cccgttctcg gagttggcaa tccggacgcc attcccaacc tgaccgagct
     1921 catgctcgac aaccagcgcc gggtgcagga gcaactcgcg gccatcgaac gactccgcgg
     1981 cggccccgcc ttcccgacgc cgggaactcc gccggccggg ggtatgccga ccggtcccgg
     2041 cgccgtgccc gccccgccgc cggggccggg cgtcataccc acctacccca gccagccgcc
     2101 cgccgcgccg gcgcaaccgg gccagccccc cgcggatccg gctgcggcac aaggccagcc
     2161 ggcgcaggag ccggagccgc agaagtcggt cgaggagcta ttggccgagc tcgacgagat
     2221 gaccggcctg gactcggtca agcgcgagat ccaccagcag gtcgccattc tcaaggtcga
     2281 gaagaagcgt tccgaggcgg gcttgaagac cgccaccatc acccggcacc tggtcttcgt
     2341 cggcaacccc ggcaccggca agaccaccgt cgcccgcatg gtctccggca tctatcgcgc
     2401 cctcggcctg ctcaccaagg gacacctcgt cgaggtcgac cgcagcgaac ttgtcgccgg
     2461 ctacctcggg cagaccgcga tcaagaccgc cgaggtcgtc accaaggcca tcggcggcgt
     2521 cctcttcatc gacgaggcct acggcctgtc cggcgatcag tacggccagg aggccatcaa
     2581 caccctcgtc aaggagatgg aggacaaccg caacgacctc gtcgtcatcg tggccggcta
     2641 tcccgggccg atgatggagt tcatcgccca gaaccccggc ctggccagcc gcttcaagac
     2701 gatgatcgag ttccccgact acaccaacga cgaactgatc gacatcatgg cgggactggc
     2761 cgcgaagtcc gactacgacg tcaccgacga ggcggaagcc gttgtccgcg agatcctttc
     2821 gcgcaccgaa cgcaatttca ccttcggcaa cggccgcttc gtgcgcaatc tgctcgagga
     2881 agccatcggc cgccacgcct ggcggctgcg cgatgacgag gacgtcacca ccgaggactt
     2941 gcggcggctc ctcgcggagg acttccgcga ggccgaggaa cacctcgccg aggacgagcc
     3001 ggcgggtccg ccgccggccg acgacaccgc cgaagcggag gccgagatga ccgccgaggg
     3061 cggaccggag cccgacgccg aagggccgga gcccgacgcc gaaggaccgg atgagaacaa
     3121 ggagtcaggg gtatgacgca gatcgcctcc acacccacga ccccgtcggc cgtcgggggg
     3181 cagcccggtg gtgcggtctc gaccgccacc agcggcgccg ccgaacgccg cgcccagtcg
     3241 cgccgccact acttcaccgg cacccccggc cggatgcgcc tgatggcggc gatcgccagc
     3301 gccctgtgcc tggcgttcgc gcttgcgggc ttcaccggtc tgcgcggagt gcagacctcg
     3361 gtggaccgcg ccagcgccaa caccgaacag gtggtgcgcg cccgcgccat ctatgccgat
     3421 ctgctcgccg ccgatgccgc cgtgaccaac ggcttcctcg tcggcgggtt ggagagcgcc
     3481 gcgagccgca agacctatga cgaggcgatg gcccgggtcg ccgagaacat cgcgaccgcg
     3541 gccagcgccc aagaggccga cggcaaggcg ctggccgctc tcaacgagga ggtccagaac
     3601 tacgccgcga acatcgaccg ggcacgcacc tacaaccgtg agggcaaacc cgttggggcc
     3661 caatatcttc ggatcgcggg cactgaccta cgcgcccagg ctctgccgat cgccgatgcc
     3721 gtcgccaccg ccaacgaggg ccgcgccgag gacgcctacg gcatcggcgc tcccgggtgg
     3781 ctcgcgctgg ggaccgggct cgtcgcactc ggcggtctgc tcgcgatcgc ggtctggctg
     3841 gcccgccgca cccaccgcta cgtcaatctg cccctgaccg ccgccatcct gctcgtcggc
     3901 gccgcgctcg gtctcgcggc gtggcatgtc acgcaggtcg cgaacaccct caacgacgcg
     3961 cgcaacggcg agtacaaggc ggccaccgcg ctggcgcagg cgcgggcagc ggcatacgac
     4021 gcgaaggcca acgagtcact gacgctcatc gctcgcggca gcgggcagac gttcgagaag
     4081 gcctaccagg actcgagcgc caagaccctg gacaagctgc aggagctcgt caacgccgat
     4141 cccgcacagg acagcctgcg gtcgcggttc ggggcctaca cggtgaagca ctccgcgatc
     4201 cgcaaggccg acgacggtgg cgactggacg ggtgcggtca aactcgccac cagcgatgcc
     4261 gccgactcgg ccaatgacac cttccgccag ttcgaccaag ccgcgagcac cgtgctcgct
     4321 caggaggtca agagcatcac caccgccttc gaccgcatcg gcggctggct gctgccatgg
     4381 gtggccggcg gcgcggcggc gctggccgcg ctgctcgcgg gccgatcgat gtcgcgccgg
     4441 atcgaggagt accgatgaac cgtatgccga gacccccctc gccgttcaag gccggcgggg
     4501 tggcgctgct cgccgcgctc gggctcgcgg cctgcgccgg cggggactac cccgacaccc
     4561 ccctcccgac gtccgcgtcc gcctcgtcgt cggcgacgac ggcagctccc accacacccg
     4621 tctcgtgcga caacgcgacg cagacctatg acccgctcgc ctcggtgccg gcccgaagcc
     4681 agatcaccga cggggcgatg tccgacatca tccagcgggg ttatctgcgg gtgggggttt
     4741 ccgccgactc ctacctcctc ggggcgaaca acccgcgcac cggacagatc gagggcttcg
     4801 acatcgacgt ggcccgggcg atcgccgagg cgatcttcgg cgacccgaac aaggcgcgac
     4861 tcgtcgtgat cacggcagcg gaccgcatac cggccctcca ggacggacgc gtcgacctcg
     4921 tcgcccgcaa tatgaccatg aactgcgctc gctggcagga catcgccttc tccagcgagt
     4981 actaccacgc cggccagaag atcctcgtgg gcatcgacag caaagcgacg tcgctcgacg
     5041 acctcaaggg ccaacgtgtc tgtgccccaa cgggaaccac cagcctcgcc aagctccagg
     5101 aagtcaacga ccaggtgggc ggcatctcga tcgtcacagc cgccaaccac accgggtgcc
     5161 tggtgaagtt ccagcagggc gaggccgacg cgatcaccgg cgacgacacg gtcctcgccg
     5221 ggcttgccgc gcaggatccc tatgccaagg tgcccgactc ggaggcgatc accgacgagc
     5281 cctacggtct cggcatcgcc aaggagaaca cctacttcgt ccaatatgtc aacgccgtcc
     5341 tcgacgagat gcgcgccgat ggacgctgga ccaagatcta caacacatgg ctcgcgccga
     5401 gcctcggcaa ggcgcccaag cccccgacgc cggtctacgg ccgctgatgt ccctcgacct
     5461 cgggatgacc gcgtcgcccg gtggcactcc gcgtgccccg ggccggctcg gcgtgcccgt
     5521 caccgccgcg gaggcgctgc gttatctgac cgacctcggg ctgtggctcg acgagcggcg
     5581 caagcaactc gacgccatcg accaggccgc gctcgcggcc ccggatcaga ccgaccgcga
     5641 ggcgctcacc cacgacatca cggtctcgat ggcgctgtgg aaggcgatct cggaccggta
     5701 tgaactgctg cgggtcgcct tcgactccgg ccgggtcggc ccggtgcagg ccgaacaact
     5761 ctcgacactg atctgggggc gcctcgatgt cgcgccgggg cagggccggg aggcgacccg
     5821 ggcgcggatg cccagtggtg ccgagggcac gctggcggtc tcgttgcccg aggcgtgccg
     5881 gctctccgac gcgatggcct cctcactacg cgcccgattg gccttggaga caacggggct
     5941 cgacgtcagc atccgggtca aggcgatccg cgaaggggtc gaacgcattc gcgaccagat
     6001 cgcgatgatg ccgctgggca ccgcgcgcga gaaggcccag cacgagttgg tcggcaccga
     6061 gcagcgcctg ttcgacgtca ccgaacgggc caaacggggt gccgatgtcg gcggtttcct
     6121 cggccccttg gagatcgacg tcgcggcccg tgaacgcgac ctgatcatcg ccgcctcccg
     6181 ccgccacgag tcggcacgcg atgccgcccg cgcgcgtgaa ctgatcgccg accttgccgc
     6241 gcgcggtgac gcgatccgtt ccctgcaggc ccagtgcctc caaagcgtct ctcccgcacc
     6301 caaactcgcc gtgcccgatg tgcgtgccct gggcgcggtg ccggaggacc ccgatgccgt
     6361 cgacgcgctg ctcgcgcggc tcgccctcgt cggcaaggcg ctcgatcagg cccacgagac
     6421 gtatgcccgc gcgctcgccg aacgcgatga actcaccggt ctcgtcggtg ccctcgacgc
     6481 ccaggtggcc gcctcgccgg cggtcagcga cgccgaacgc gccgacctgg cgccgatgcg
     6541 cgcccaactg gccgccacct tggcggccac cccgatcccg ctggtgcggg cgcgggcgct
     6601 gatggcggca taccagtcct atgtccaagc cgtgaccacc aacaccggaa gggcaccgcg
     6661 atgagcgagc tgaccgcctg ccgacagcct ggctgcacgg gaaccgtcgc cgacggctac
     6721 tgcgatgtct gcggtatgcc gggtgagccg gaccagatcg ccgctgccgc agcgccaccc
     6781 gccccggccg ccgcgcgacc cgccccggcc gccgcggctc cggccccgcc gcccccggtt
     6841 gcccagccgt ccggcgtgcc gccgtccccg tggcgccgcg gacaagctgg cgcaccacgc
     6901 ggcgcgggcc cctcctgccg ccagcccggc tgcaccggca ccatcgtcga cggctactgc
     6961 gatgtctgcg gcacacccgc gacgcccacc tcgaccatgg ccacgcagcc ggtcggcgcc
     7021 ggtgagagcc cggtcgaggc cagcaactcg caggtctcgg cccggctgga tgccaccgcg
     7081 ctcggctccg agcgggcccg cagcggcggc accatctcca ctcgccgggt ctcgggccac
     7141 agccagcgca tgcgcaccgc ccggatcggc gccggcgtca ccagcgtccc gccggtgccc
     7201 gagattgatg ctgccaaggc tgtcatcaag aatcccgttg tcccggaaga caaacgcttc
     7261 tgccccaagt gcggcgagcc cgtgggacgc gggcgggacg ggcaggaggg gcgcaccacc
     7321 ggcttttgcg ccaactgccg cacgccgttc gacttctcgc cgaagctcaa gccgggagac
     7381 ctcgtcgccg ggcagtacga agtggccggc tgtctcgcgc acggggggct gggctggatc
     7441 tatctggccc gcgaccgcaa tgtctcgaac cggtgggtgg tgctcaaggg cctgctcaac
     7501 tccggcgacc ccgacgcact ggccgcggcg ctggcggagc agcgcttcct cgcccaggtc
     7561 agccacccca atatcgtcga gatctacaac ttcgtctcgc acgagggcgc cggctatatc
     7621 gtcatggagt acatcggcgg cacctcgctg aaatcccttc tcaagcaacg gatgcgggcc
     7681 gccgggaagt atgacccgct gccggtggac caggcactcg cctacatcat cgagattctg
     7741 cccgcattcc agtacctgca cgacctcggg ctcgtctatt gcgacttcaa gccggacaac
     7801 atcatccaga tcggtgacga ggtgcgcctg atcgacctcg gcggggtgcg ccgcctggac
     7861 gaccaggact cggcgatctt cggcaccgtc ggctaccagg cgcccgaggt cgccgagcac
     7921 ggcgtgagcg tcgcctcgga catctacacg atcggtcgca ccctcgtggt gctgacgatg
     7981 gagttccggg ggtatcagac caagtacgtc aagtcgctgc ccccggtcga ggaggtggcg
     8041 gtcttcgagc aatacgactc cttctaccgg ctcctgctca aggcgtgcgc cccctcgccc
     8101 gacgaccggt tcgtctccgc cgacgagatg cgcgtgcaga tgctcggcgt gctgcgagag
     8161 gtggtcgccc aggacaagaa cgccggtgcc gcaacgacat ccgcgccatc ggtgctcttc
     8221 gcgccgccgg cagccggtga cagccacctg acgtatgccg acctgccggc gttgcatccc
     8281 gactcgagcg accagcagat cggctggctc gcgacgatcg cggcgcagga gccggcccag
     8341 cggctgcggg cgctcgcgtc ggcgccggga gccacgcccg aggtcttcct ggcccaagcc
     8401 cgcgcggccc tcgaactcgg ccagtgggag gtgctcgacg gcgtcgtgca acggatgctc
     8461 gccgaggacc cgtgggagtg gcgggcggta tggatgcagg gtctggccgc actggcccgc
     8521 aagtcaccga cggaggcgca gagcgccttc aacgccgtct acgggcaggt ccccggagag
     8581 ctcgcgccca agttcggcct ggccatcgca tgtgcgttgt cgggcaatga cgagatcgcc
     8641 gagcggcttt acacgatctg cgctcgcacc gacgcggcat acgtcccgca ggcggccttc
     8701 gccctggcgc ggatccgggg cagtcgcggc gacaccgacg gcgtgcagca agctctgacg
     8761 ctcgtgccac ccaactcctc ggcatacccg ctcgcgcgcc agcactgggc cgagcacctc
     8821 gcggcacagc cgcgcgacac cgccgacctg accctcgccc tccagaccgc cgacgacgcc
     8881 ggattcagcc gccgccggcg cgccgaactc ggcgtcgaca tctacggcaa ggccctcgcg
     8941 cgggtcgagg agagcggccc gaagccgggg gagcggatcg gcgacttccc ggtcaccgcg
     9001 gacggtatgc ggtccggtct cgaacgtgcg ctgcggctgc tcgccgacgt gacggacaac
     9061 ccgctggagc ggatgcggct gatcgacaag gcgaacgaca tacggccgtg gagtctcgtg
     9121 tgagactgcc ctggcgcaag aaggaggaga aggtggctga gtcgtcctgt ccggcctgcg
     9181 gagccacagt gccccccgac gcaagcttct gcgaggcatg cggcgccgac atcgcgaccg
     9241 gtccggggcc cgccgctgcc gcgccaggag acccgggtcc ggctgcggca caagggaatc
     9301 cgggagctcc cttcccgccg gcgggctctc ctgggctgcc tgccgtgcct ggctcgccgg
     9361 ccgcgcccgc acccgcggcg aacgcgacgc ccatcatgcc cggcgccccg gtgaccacgc
     9421 ccccgcctcc gccggccccg caaccggtcc cggagggcac tacgtcagcg cccgctgacc
     9481 cccggccggc ccccgggcag ggaccgggac ccggcggccc ggccccggtg agcgagcaca
     9541 atgtcgaccc cgcgtcgctg ccgcaacccg aggcggccgc cacgcccgcc ccacccgcgg
     9601 agggcgagga gtcgccgctc gatatcgggt ggaccggtgt cgtgcccggg gcggccagtt
     9661 cgtatgacgc cacgcccgtc gccgtccggt gcggaacctg cggcgagggc gtctacaccg
     9721 acgggtattg cgaccagtgc ggcgcgaagg aacccaatcc gcgtgaccac ctcgaggaga
     9781 atgtcgcggt ctgggtgggc ggggtcagcg acatcggccg gcgtcactcc cgcaacgagg
     9841 acgcgctcgc ccttgccgca caagacgatc cggtgtcctg ggccgtgctg gtggtgtgcg
     9901 acggcgtctc caacaccacc gactccgata tcgccagtct ggcggctgcg cgggccgccc
     9961 gcacggtcct cgagcagccg ttgagttccg ggatgggtgt cgccgcggcg gcccaggccg
    10021 cggccaccaa acgcctcggc gaggccgtgg cagccgccaa cggggcagtc atcggcaaga
    10081 ccaagcaggg cgacgaacac tcgccgtcgt gcacattcac cggggccatc gtcgacgggc
    10141 ggaccgcata tgtcgcgaat gtcggtgaca gccggatcta ttggttgccc gacagcggcg
    10201 gcggcgagca gctgagcatc gacgactcgg tcgccgccga acagatcgcc tcgggcatcg
    10261 agcgcaaggt ggccgagacc gggccgatgg cccattcgat cacccggtgg ctcggcatcg
    10321 acgcgcccga cgacctcacg ccccacaccc gcaccatcca gctgtcggaa ccaggctggc
    10381 tgatgctgtg caccgacggg ctgtggaact actgctccga ggccggcgcg ctgcacgaca
    10441 agatcgtgga gttccaggcg aacggcacgc cggcgcaacc actcccgctg gcgcgggccc
    10501 tggtcggatt cgccaacaac gccggcgggg ccgacaacat caccgtcgcg ctggcccgca
    10561 tcccggccga cggcgaggac gtcgccgccg ccggggccgt gctgccggcc cccgcccccg
    10621 aggcaacggc tgaggtgcct ccggtgccgg gggaaacgtc ggacgcgacc gcgcccgacc
    10681 cggccggcgt cacggacgcg tcaacgcaag cccccggcat accggctgaa gagtccggca
    10741 caccggcggg gccactcgag gcacctgctg aggcgcctgc tgagacaccc gctcaggcac
    10801 ccggcatacc gggtgagcag ccctcgatcc ccgccgaaca gaccgcggcc ggcgcggcgg
    10861 cgccaccgac cggtgagacc caggacaccg aatccgcggg ggatttggca ggatcggcgc
    10921 atacagagaa ggggcagtga tggcgcagtt cacagctgag gcattccaga accagtacct
    10981 cccggacggg gcgaccgatg tgcacgccat cgtgcgtgtc gacgcatcgg gcgcgacgac
    11041 ggccgccgcg atgagcgggc agggtgcggc cgaggtgatc atcgtcgaca cctcgggttc
    11101 gatgggggag aagggcatcc gcgagggtgc cgtcgccgcc accgcggcct tgcaggagat
    11161 cgcggacggc accaacttcg ccgtcatcgc cggcaaccac ctcgcgtggc aggtctatcc
    11221 gagcgcgccc gtgcccggga cggccatcat ggacccggcc aaccgcgccg cggccatgaa
    11281 tgcggtgcgc ggcttccggg ccgacggcgg cacggcgatg ggtcagtggc tgctggcggc
    11341 tcgccaggtc ttcctcaccg tgcccgacac ctatcgcaaa cacgcgatcc tgctgaccga
    11401 cggcgccaac gagcacgaaa ctccggacca actgacctgg gcgatccagt cctgcaccgg
    11461 ggcgttccag gcggactgtc gcggagtcgg tgaccggtgg caggtcgatg aggtgcggcg
    11521 catcgcgtcg gcgctgctcg gcaccgtcga cctcatcccg cagccggagc agatggccga
    11581 ggagttcaag accctgatgc gggcctcgat gtcccggggc gttgccagcg ccgaactgcg
    11641 ggtatgggcg ccgcagggag cgcaggtgct cttcgtcaag caggtctcgc ccaacctcga
    11701 ggacctgacc acccggcgcc gggaggtcaa cccgctgacc ggcgcctatc ccaccggcgc
    11761 ctggtcggac gagtcgcgcg actaccacgt cgccgtgcgg ctgcccgcca aatccgttgg
    11821 gcaagaacag cttgcggctc gcgtccagat cgccatcggc gaggaggtcc aggcgcaggc
    11881 gctggtcaag gccatgtggt ccaatgacga cgcgttgacg gccaggatca acccgcaggt
    11941 ggcgcagtac accggccagg ccgaactggc gcaggccatc caggacggcc tggcggccaa
    12001 ggccgccggc cgcgacgacg aggccacgag caagctcggg cgcgcggttc agctggccaa
    12061 tgaggccggc aacgccgaga tgacgacccg gctgaagaag gtcgtcgaca tcgacaacgc
    12121 cgagaccggg accgtgcggt tgaagaagca ggtggagaag ctggacgaga tggccctcga
    12181 tactgcctcc accaagaccc agcgggtgcg caagtgagcg ccgtctgccc gaatgggcac
    12241 gacaccgtct cgacggacta ctgcgacgtc tgcggcagcc cggtcgcggc cggcgcgtcc
    12301 agcgaggccc ccgcggcggc ggcgcccgct gctgccgcgt ccggcaactc cctcgacctg
    12361 ccacccgtcg gcggggatag tggtcccaag gactgtccca actgcggcac tcccaacgcc
    12421 tcctccagct tgttctgcga agcctgtggc tacgacttca ccaccggagc gttgccggcg
    12481 gcggcgccgg cgagcctcga gggcgtcagc gcggccggcc tcgaaagccc cgcccctccg
    12541 gtgcgcgagc cggccggtgc cggtctggct cccgagtccg agcagccgcc ggcgttcgcg
    12601 cccgacgtcg ctgcggccga gaccgagtcg gaagccgagg gcacgcctga tggcagcggc
    12661 gagctcgccg ggctgaacgc tgccgaggag ctcgacgccg acggcctgtc ggtatccgcc
    12721 gaaggcgaac cgggagacgg cgaacccggt cccggtgagc cgggggacgg cggcataccc
    12781 gccccggacg cgcctgagag cccggaggcg cccgacggtt ctgacgcccc cgagggtgca
    12841 ggagcgttcg agggtgcagg ggcgtccgag ggtgcagggg ccccgagtga gcccgctccg
    12901 gtctcgggtg aagccgaggc gggtgctggg gagcagccgc cggtgctcgg cggggtggac
    12961 ccgaacgtgc cgccgcggat ccccttccag tgggtcgccg aggtgtggat cgacccggac
    13021 tggtatgccg atcagcaggc cgaggagccg ctgccgtcgg cgggcctgcc cgttgtcgtc
    13081 ccgctgcgga tcgcgagcgc gctgatcggg cgggcgtcgg cgagccggaa tatcgtgccc
    13141 gacatcgact gctctgccga cccgggtgtc tcccggcggc acgcgcagct gacgacggac
    13201 gggtcgcggt ggttcgtcga ggacctgcag tcctccaatg gcacgttcgt cgggccggcg
    13261 tcggggccgc tccccgatga cccgatcgcc gtcggcccca agaccgaggt cggcgacaac
    13321 cgcatctatg tgggggcgtg gactcggatc gtcgtgcgac gcgcgaccga tgaggagatc
    13381 gcaatcggat gaggccgctg accttcctca ccatcgagca cgaggatcag tgcccaccct
    13441 cgtggctcgg ggagtggtgg gaggagcgcg gcgtgacact gcgccgggtg cgagcgcacg
    13501 tgcacccgga cggcgttgcg gcagagatga ttccggctga tgtgggcgac gctgacgctc
    13561 tcgtggtcct cggcggcgag gcgggggcga acgacgacgc ggactacccg tggctccccg
    13621 cgacccgcga gctgatcgcg cggagtgtgg agggcggcat accgttcctc ggggtctgcc
    13681 tcggccacca actggcgacc gtggccctcg gcggcgaggt gcggcgcaat ccggccggga
    13741 tggccaccgg gctcactccg gtggcgctca ccccggccgg agccaccgat ccgttgctcg
    13801 gcggatgcga cggtttgcgt gtcgtgcaat ggaacagcga catcgccacc cggctccctg
    13861 agcgggccgt gcagctggcc acggcacccg acggcactcc gcaggcggtg cggttcgcgc
    13921 catgggcgta tggcgtgcaa ttccatcccg aggccacacc cgagcaggtg gactcctggt
    13981 gggtcgacaa gccgtcggcc gccgaagccc gggagcgcgc cgcagcggca tccgcggcga
    14041 tccacgcggc ccgcgacgaa ctgcgcaccg cctggcgccc gtttgccgcc cgcttcgtcg
    14101 acctggcccg ggacttccgc gccgggcggc tggacgcgcc ggtcacgaca ggcgactagg
    14161 gccggcccga tgccgccgac agacctcggc cgccggccag cgggcggtat gccgcgcacg
    14221 gccgccgtcg cgtcgcttgc tgcggtgctg acctatgcgg tcggttcggg gatcgcgtcg
    14281 gcgctgcaac ccaccgggta tgccgccgac cagcagtcgg tcaccgactt gctggcggac
    14341 ggggtgccct tccggtgggt cgcggtcggc actttcgtcc tgtccggact aatggtcgtc
    14401 ctggccgcga tcgccctgcc cgttgcgcgg tcgcgccggg gtgtcctcgc cgtaggcggc
    14461 gcagcggtga cggtgctcgg gctgttgccg cgcgattcga tggcggtcgt cgaggctccg
    14521 ctcctcggct gcgccctcgt ggcgctgctc tcgctcgcct gctggccggt ggccggccgc
    14581 agggcgcgcg agggagatcg gatccgggcg ggcgtgctgc tggcgctcgt ggccgggctg
    14641 gggctggccg cggtgggcga tctgggttac ggggcttacg agcgcgtcct ggccgtggcg
    14701 ctgctgggac atgtggcgct cgcggccctg cacgcctggt gggtggccgg acatcgcctc
    14761 gggtcccggc cggtgcggat ggcggtcgcg gcggtggtgc tcggtgccgc ggggatggtg
    14821 ggcgggatcg tcacgacggt ggtcatgccg gcgcacgtgt ccatgcagta tgtcgacatc
    14881 cggctcgcac tctcgccctc cccgtccgat ctcgggcggg tcgtcgtgcc gacggtgctc
    14941 ggagacctcg aagccggatt cgccgggatc gcgcccggcg tgcgcgcctt cccgcaggtc
    15001 aaggccgacg tggtcacctc gatcggcagc ggggacgcgt cgttggagac gttgcggccg
    15061 tcgccggccg aactggaccg cgccatccgg gacgctgcca tcgggctcgg gtttcgtgtc
    15121 ggggtgggtg cgctggtcgt catcgggttg gccatcgccc tgcgcgccac gacgatgcgg
    15181 cgtggcccgg ggctgggcac ggcgttggtc gttgtcgctg ccctggtgct ggcactgggg
    15241 acgatcgcgg ggaccgtggc gcaaacctat cgatcgggca cgccgcggga gttcaaggcc
    15301 agcgggttgc tcggcaccat ccagcagaac tcgagggtct tcgacgacgt cgccgtgcga
    15361 tccgagcagg tcgcgccgta cttgcgcaat gtggtggcct tgagcgaggc gttgcgggaa
    15421 cgctacgagc cgcaaccgat cggcggcgag agcgtgctgc ggctgctgct ggtctccgat
    15481 atccacgcgg gcaaccagta ccccctgttg aagaacctca tcgagaccga gcagatcgac
    15541 gcggtcatcg attgcggtga cctggtcaac ttcgggacgg tgaccgaggg ggagttcgcg
    15601 ggagtcttcg aagggatcgg ctcgctgggt gtgccgtatg tcttcgtgcg gggcaatcac
    15661 gatgccacct cagcagccga cgacgcggtg ctgcggcgac tcgaccgcat acccaatgtc
    15721 gttctcctgc aaccggacag cacgcgatac gaggagttca cgatcgcggg cgtgcggatc
    15781 gccgggttca acgatccgcg gtggttcggc gacgacggac acggctcggc ggacaaacag
    15841 gccccggccc gggaacgctg gctggccagt tttgcggggc gggaggtgcc cgatgtcgtc
    15901 gtcagccacg aaccgtgggc ggtgcagggg atcacggggg cgggtgtcct gctcaacggg
    15961 cacatgcata cggcgtttcg cgaaggcaac cgggtgcagg cgggcacctt caccggcggc
    16021 gggcccttca gccactacat caccggcgag gccggcgagg agttgcgcgg gcagccg
//
LOCUS       DAOPJG010000063        16012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3845_length_16012_cov_7.657768, whole genome shotgun sequence.
ACCESSION   DAOPJG010000063 DAOPJG010000000
VERSION     DAOPJG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 16012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16012
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3845_length_16012_cov_7.657768"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..736
                     /locus_tag="PKU75_07645"
     CDS             272..736
                     /locus_tag="PKU75_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPF80786.1"
                     /translation="MNISARTDYAIRALLALADAQAQDAGPVSAEALAGAQGLPKKFL
                     EAILLDLKRGGLVSSRRGSAGGYQLATGADEIRLGAVVRIVDGPLAEVRGIRPQDAAY
                     DGVAQHLPVVWVALRASLRSVLDEVSLADVLNGDLPEQVRELAEHPDAWRNR"
     gene            986..1603
                     /gene="rpsP"
                     /locus_tag="PKU75_07650"
     CDS             986..1603
                     /gene="rpsP"
                     /locus_tag="PKU75_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPF80787.1"
                     /translation="MAVKIRLKRMGKIRTPFYRVVVMDSRTKRDGRAIEEIGKYHPTE
                     EPSLIDINSERAQYWLTQGAQPTEAVEALLKITGDWQKFKGEPGAEGTLKVKAATASK
                     KELYEAAVAAAGKSADAGPATTPRKAAKKAEEAPAAAPAAEAPAAAEAPAAEAPAAEA
                     LAAEAPGADAAPAADTDGSGAAADSVDADADATTTAEAAAEVAEQ"
     gene            1606..1848
                     /locus_tag="PKU75_07655"
     CDS             1606..1848
                     /locus_tag="PKU75_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPF80788.1"
                     /translation="MLEEALEHLVTGIVDHKDDVAVRSKDLRRGSLLEVRVHPDDLGR
                     VIGRSGRTASALRTVMSALAGGQNVRIDIVDTDRVR"
     gene            complement(2071..4686)
                     /locus_tag="PKU75_07660"
     CDS             complement(2071..4686)
                     /locus_tag="PKU75_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HPF80789.1"
                     /translation="MASPLARAASLKIKLGALVAVSVLFATVIATVGANSKVTPWLVV
                     PVTIAVALAVTQWLAAGMTAPLRAMTQAAGRMAQGDYTSAIPVGGNDEVADLARAFRA
                     MATDLQDADQQRRQLIATVAHELRTPAAALRALLENMADGVTPADPANLESALGQADR
                     LSDLLTDLLTLSRLDAGITDLRLESVAVQELLDQVVAEYAAGGRPVTTQIDVRPPTLT
                     VRAEPARLRQAVTNLLDNAARHTPSGTTVTLTAATAAPDRWHLDIADEGPGIAEADRD
                     RIFERFGTDSAGGTGLGLAITRWVAEIHGGTVAAIDPQPGRRGARLRLTLPTDPPTRI
                     RPDSVAGVRMPPAPPTPPRAPSSRPPGAPIPLDLARPERAVASRPRPAEGPVIDGLFG
                     RVWPETVREGRPRLVALAALVGAMAGLLLPERDTGIAWSVVLLATGAAVVMAARRRTW
                     MTAITAVLGVGLAALATLRAAEVVVPLSILAAALLIVVTIVPVSSAYGLMGAAAAWPT
                     AGIRGLPWLGRSLARSGTATTWWRVARTATISLLALAVFGSLFASADAIFGHWVDVLI
                     PDLTVDTFVARLFVGIFFGGFALAGMYLAINPIRDEALTLPKGRPLAHPWEWLVPVGL
                     IVATYLLFIIAQASAWLGGHDFVQRATGLTYADYVHQGFGQLTVATVLTLVVVALTVR
                     KVPQADPGDRLLLRVVLGLLCILTLAVVASALWRMHLYQQAFGYTTLRLLVDFFEAWV
                     AIVVVMVMVAGITFRGRWIPTAAVVAGAALVLCFGLLNPDAWVAERNLQRYAATGKLD
                     GSYLTSLSADAAPAVAASTLSPADKRCILGNGPGQFLTEDDALEWNLGRVRARAALAQ
                     VGPGQASRSCAPIGS"
     gene            complement(4697..5377)
                     /locus_tag="PKU75_07665"
     CDS             complement(4697..5377)
                     /locus_tag="PKU75_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF80790.1"
                     /translation="MPTVLVVEDEPVINQAVSDRLRAEGYDVVSAFSGPEAAELHAAH
                     RPDAIVLDIMLPGFDGIEVCRRVQTERPVPVIMLTARDDESDILVGLGVGADDYLTKP
                     FRMRELVARVKALLRRVERARELAASPASRMTVGDLVVDPTTRRVTLQDREIHLTPLE
                     FDLLAMLAASPGHVLTRERLLEEVWGWADASGTRTVDSHVKGVRAKIGADRIRTVHGV
                     GYALEPLS"
     gene            5465..5995
                     /gene="rimM"
                     /locus_tag="PKU75_07670"
     CDS             5465..5995
                     /gene="rimM"
                     /locus_tag="PKU75_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142763.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPF80791.1"
                     /translation="MSVLVARIGKPHSLRGEVTVQTHTDDPDGRFVPGAVFATEAQPG
                     TGVPRALTLRTSRVHKGIWLLAFDEIPDRTGAESLRGTKLFADEGEAGDDADDDGWYE
                     DELIGLRAVDPAGTELGEVVGLDIGPAQDRLILRLADGTEAQVPFVEAIVPEVDTESG
                     RVVIDPPPGLLDLGRE"
     gene            6000..7214
                     /gene="trmD"
                     /locus_tag="PKU75_07675"
     CDS             6000..7214
                     /gene="trmD"
                     /locus_tag="PKU75_07675"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPF80792.1"
                     /translation="MRIDVVTIFPEYLAPLDLSLIGKARRDDLLDLAIHDLRQFTHDR
                     HNTVDDTPYGGGAGMVMKPQPWAEALTHVGGLASGAPRLLVPAPSGRRFTQEYARALA
                     EAEWLAFACGRYEGIDERVYTYAADALGWEVVPVSLGDYVLGGGEVAVLAMVEAIARL
                     IPGVVGNEESLVEESHEGGLLEYPVYTKPATWLPQPPAAAAATAYDVPPTLLSGNHKA
                     IADWRHQQRLERTVARRPDLLEAAATVTGLEDLEGGPATPADIPELIVLTRACWVPEA
                     LDAGTVDFPPMTDGPAEHAAALQDWSTWVWRSNGRLVASGRGRRDPDDPSVWQVGRLM
                     VAPDLAGRGIGRAVLAFIESLAPADVTSYWINTARRAERNQRIYKKAGYRLAPWESKV
                     HPATVDLVKKRR"
     gene            complement(7225..7983)
                     /locus_tag="PKU75_07680"
     CDS             complement(7225..7983)
                     /locus_tag="PKU75_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF80793.1"
                     /translation="MSINPGWPVALAIVLLLAVTVAAYAVGRLPGIGSTLVAAARALA
                     QLSLAALVITAVVGSLPLSLLVLAGMFTVAVVTTVRRVGAANAWPWATVAILAGLLPV
                     AAIILLTRTIPPTGIALIPVFGILTGNTMNGHTLVGRRAFQALREERESYDAWLALGF
                     ERRAAIGKVIHRHIPEALIPGLDQVRTAGVVTLPGAFIGVLLGGGSPAQAAAAQVLVL
                     MGIMAAQTVTAVVAEELMRRSLLLPDDLRASLRP"
     gene            complement(8068..8523)
                     /locus_tag="PKU75_07685"
     CDS             complement(8068..8523)
                     /locus_tag="PKU75_07685"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPF80794.1"
                     /translation="MTETGVGANVLGLATGLAFGALLQRGGLSDSATIKNQFSGTDWR
                     VAGTVGTAVAMGGVAFEQLRRRGVVAPDVKPVKLGGILSGAALFGTGMTLGGYCPGTA
                     VAGIGAGHRDAGWALLGMSAGALGFVRAYPALKKGLDAGDFGRVRLGAA"
     gene            8869..9225
                     /gene="rplS"
                     /locus_tag="PKU75_07690"
     CDS             8869..9225
                     /gene="rplS"
                     /locus_tag="PKU75_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPF80795.1"
                     /translation="MQRFDEIDKASLKSDLPAFRAGDTVKVHVKVVEGNRSRIQVFQG
                     VCIRRHGGGIGETFTVRKISFGVGVERTFPLHTPIIDKIEVVTRGDVRRAKLYYLREL
                     RGKAARIRERRETPAS"
     gene            9305..10009
                     /gene="lepB"
                     /locus_tag="PKU75_07695"
     CDS             9305..10009
                     /gene="lepB"
                     /locus_tag="PKU75_07695"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPF80796.1"
                     /translation="MTSEAVTPETRAAGRAKRKPLPVWVHFLIALALVAVLRNFVVQS
                     FYVPSGSMIPTLEIGDRVVVSKLDNTFERGDIVVFDGTDTFGGGASQRWEGTFGKVAS
                     GIAGIFGINLGEKDYVKRIIGVGGDHLVVGEDGTLTINGKAVNEPYLAEGVKSSDYPF
                     DVTVPEGKLFMMGDNREQSADSRAHLGDPGGGMIPESDVIGSPVLRYWPLGRFGTMGD
                     SAHEPSLAELPAGSAS"
     gene            10006..10830
                     /gene="lepB"
                     /locus_tag="PKU75_07700"
     CDS             10006..10830
                     /gene="lepB"
                     /locus_tag="PKU75_07700"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPF80797.1"
                     /translation="MTSTPGAPPPHDPGPGPEARYPTHTGVRARAAEPEPDAGSSPAA
                     PRRSVGGHLAALVRELLIVGVIALVLSFLVKTFLVQAFYIPSESMESTLVKDDRVIVS
                     KLTPSLIDLKRGDVVVFADPDHWLTPELRADRGPVLNRIQDLLTFVGLLPDTAEGHLI
                     KRVIGLPGDKVACCDTGGRLTVNGVAITEPYVKPGEEPSEITFDITVPSGRVWVMGDN
                     RGHSSDSRLHDGDGKGNEGSVPIDLIVGRAVLTIWPLDRITWLSNDADTFAKVPGT"
     gene            10827..11576
                     /locus_tag="PKU75_07705"
     CDS             10827..11576
                     /locus_tag="PKU75_07705"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPF80798.1"
                     /translation="MSSVRRSTKPSLRVERALQRDGYAVLAGMDEVGRGALAGPVSVG
                     VVVIDVTCRSAPQGVKDSKLLTPGARTALVPRIHRWSTAYAVGHAWPEEIDRIGIIAA
                     LRTAGRRALAQLPVRPDLVILDGNHDWLTDPAEAGLFAAFAPDPAPPVTTMIKADLKC
                     SSVAAASVLAKVARDAMMVAYAREHPAYGWDENKGYAAPGHLAALAEHGACPLHRRSW
                     HLPGVGEVVMGDDEIDIDLLAGLGGAESEMA"
     gene            11573..11887
                     /locus_tag="PKU75_07710"
     CDS             11573..11887
                     /locus_tag="PKU75_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HPF80799.1"
                     /translation="MSSEDLEKYETEMELALYREYRDVVGLFSYVVETERRFYLANGV
                     DVAVRGEGSEVFFEVTMTDAWVWDIYRPARFAKNVRVITFKDVNVEELAKADLEVPKG
                     HF"
     gene            12046..12423
                     /locus_tag="PKU75_07715"
     CDS             12046..12423
                     /locus_tag="PKU75_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPF80800.1"
                     /translation="MPDARAEVGRFGEDVAARYLSDRGLTIIERNWRSGTPRGELDII
                     ARAPDGDLVIVEVKARRTLGVGAPVNGVTPQKLRQLRRLANTWLREHPEIPAPGVRFD
                     VVGVLIGRGSSPSVVQHIPGVVV"
     gene            12420..13964
                     /locus_tag="PKU75_07720"
     CDS             12420..13964
                     /locus_tag="PKU75_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPF80801.1"
                     /translation="MTLGATHAIALSGLQGQLVEVEAHLSNALPGLLVSGLPDAACRQ
                     APDRVRAAIESSDLALPSRKIVVNLSPAAVPKNGSGYDLAIAVAVLTAIGDVEQKHVS
                     RVVHVGELGLDGRVRPVAGVLPMVTAAASAGISDCIVPQTNLAEASLVEGIRVHGVRT
                     LVDVVHAYKEAGPPTRWPRQLVRAVTGPAARPVPDLADVSGQAAARAAVEVAAAGGHH
                     LLLTGPPGSGKTMLAERMVGILPPLRLDQALEVMSIRSLIGATHAESELDVTPPYVAP
                     HHGASLAAMVGGGSGLVRPGAVSAAHRGVLFLDEAPQFKQDVLQALRQPLESGRVVIA
                     RARATVDFPARFQLVLAANPCPCGHGHGKAVRCTCTPMARRAYAARLGGPILDRIDVQ
                     VHVPPVTRAANTAAAGEASAVVAERVREARAAAAQRWGPASGGRWLTNSEVPGPVLRV
                     GSFRLPGPVTADLDRALERGALTMRGYDRTLRVAWTRADLTGRSVPVADDVGLALLLR
                     AGAQAA"
     gene            13966..15102
                     /gene="dprA"
                     /locus_tag="PKU75_07725"
     CDS             13966..15102
                     /gene="dprA"
                     /locus_tag="PKU75_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPF80802.1"
                     /translation="MSIANPYEHVLRDERSARIAFARLAEPADPAVAEVVATEGYAGA
                     LRGVLSGRHTKLSRKLAPRIGHLDQIDDDAIAAALQARIVVPGDPAWPRGLDDLEAAP
                     HCLWVRGGAAEALDNLCTRSVAVVGARAATAYGLDRAADLGAELSERRFTVVSGAAIG
                     IDAAAHQGALAVEAPTVAVLACGIDRIYPRSHEVLLQRIAETGAIVTELPPGAAPYRQ
                     RFLQRNRLIAAMTCGTIVVEAALRSGSLATAAAATQLARPVGALPGPVTSMCSAGCHQ
                     AIREGIASLVTDAADVADLLGSFGRDTAPLRRAPVLPEDELPGTQRSVWEVLPVSRPA
                     SVAALAERSALDELAVQAALGPLELAGLARRSGEHWMRASRVAP"
     gene            15134..>16012
                     /locus_tag="PKU75_07730"
     CDS             15134..>16012
                     /locus_tag="PKU75_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPF80803.1"
                     /translation="MESDTAVVAAFARHLGAERGRSAHTVRAYEGDLRSLAAYLDPPG
                     ANARPSGGEGKSHLLAATLTDLRGWLGAQAGEGVARATLSRRAASVRTFFDWALQTGR
                     LETNPALRLVSAKRSRRLPTVLSAEAAGEVLDAAGVAADDDEPLALRDRAMVELLYAS
                     GIRVGELTGLDVDDVDLDARVATVMGKGARERTVPFGAPAGAALERWLRVGRPALAGA
                     GSGPALFLGRRGRRVDQRRVREVVHATTSRVPGGAEMAPHGMRHTAATHLLDGGADLR
                     TVQELLGHRSLATTQLY"
BASE COUNT         2368 a         5653 c         5723 g         2268 t
ORIGIN      
        1 aactccggtc cgggttgcgc gcaagtcccc gtggcgacgg gctgccgcgg gcggggccga
       61 cccgtgtcct tctcggagac gccgcgcccg tgagtagccg ccaactccgt gggaagccgc
      121 caactgcgtg agaagccgcc aactccgtgg gaagccgcca actccctcag gtatttgtgc
      181 gggagttcgc gggatctgag gcggttcgcg ggatctcagg cagttggccc gcgcggcgcg
      241 ggccgcacgg ggaaagtgcc aggatgacgg cgtgaacatc tcggcccgca ccgactacgc
      301 gattcgcgcg ttgctggcgc tggcggacgc acaggcgcag gacgccgggc cggtttcggc
      361 ggaggcgttg gcgggggcgc aagggctgcc gaagaagttc ctcgaggcga tcctgctcga
      421 cctgaaacgg ggcgggttgg tctcgtcacg gcggggcagc gcagggggct accaactggc
      481 cacgggcgca gacgagatcc ggctcggggc cgtggtgcgg atcgtcgacg ggccgctggc
      541 ggaggtgcgc ggtattcgtc cgcaggacgc ggcatacgac ggggtggccc agcacctgcc
      601 ggtggtgtgg gtggcgctgc gggccagcct gcgcagcgtg ctggacgagg tgagcctggc
      661 cgatgtcctc aacggtgacc tgcccgagca ggtccgcgaa ctcgccgaac atcccgacgc
      721 ctggcgtaac cgctgaccgc cccgcgtcgg ccgccgacgt cgatctgctc gccgatcgac
      781 tcggccctgg gtcagttcgc cgccagtccg gttgtgccgg cctcgcgggc ggtggcgtca
      841 ccagcggtgt tggacgcgtg ccacaatgga ggttcgtaca ccttcgcctg cgggtcgacc
      901 gcggcgatac ctaccccgag tcacggcccc ccgggcactc ctgcgcggtc ttgccggact
      961 catatcggaa agagagacca cacacgtggc cgtcaagatt cgtttgaagc ggatgggcaa
     1021 gatccgcacc ccgttctacc gcgtcgtcgt catggactcg cgcaccaagc gcgatggccg
     1081 ggcgatcgag gagatcggca agtaccaccc taccgaggag ccctcgctca tcgacatcaa
     1141 ctccgagcgt gcgcagtact ggctcaccca gggcgcgcag ccgacggagg ccgtcgaggc
     1201 gctgctgaag atcaccggtg actggcagaa gttcaagggc gagccgggcg ccgagggcac
     1261 cctgaaggtc aaggccgcca cggcaagcaa gaaggagctc tacgaggccg ccgtcgcggc
     1321 tgcgggcaag agcgccgacg ccggccccgc caccacgccg cgcaaggcgg ccaagaaggc
     1381 cgaggaggcc ccggccgctg ctcccgctgc tgaggctccg gccgctgctg aggctccggc
     1441 tgctgaggct ccggctgctg aggctctggc tgctgaggct ccgggtgcgg acgcggctcc
     1501 tgccgccgac accgacggat ccggcgccgc tgccgacagc gtcgacgccg acgcggacgc
     1561 cacgacgacg gccgaggccg ccgccgaggt cgcagagcag tgaccctgct cgaagaggcg
     1621 ctggagcacc tggtcaccgg catcgtcgac cacaaggacg acgtcgccgt gcgatccaag
     1681 gacctgcgcc ggggcagcct gctggaggtc cgggtccacc cggacgatct cggccgggtc
     1741 atcggccgct cgggccggac ggcgagcgcc ctgcggaccg tcatgagtgc ccttgcgggc
     1801 ggtcagaacg tccggatcga catcgtcgac accgaccggg tgcgctgagc gccggttcga
     1861 cgaatcacgg ccgaggggct ggatggctga gccgtccggc ccctcggccg ctttgcgtgg
     1921 gggcgccgag caaggggagg cagggtgccg acgacctcca ccccgtgagg ctcgcggcgc
     1981 cgagcgttgc cggattcgag ggtttcgggg gcgaggtttt ccggacccga tcggtgtcag
     2041 gacccgatcg gtgtgaggac ccgatcggtg tcaggaaccg atcggggcgc aggagcggga
     2101 ggcctgcccg ggtccgacct gggcgagggc cgcacgcgcc cggacgcggc cgaggttcca
     2161 ctccagcgca tcgtcctcgg tgaggaactg gccaggaccg ttgccgagga tgcatcgctt
     2221 gtccgctggc gacagagtcg acgcggcgac cgcgggggcg gcgtcggcgg acaacgaggt
     2281 caagtagctc ccgtccagct tgcccgtcgc ggcataccgc tgcaggttgc gttcggccac
     2341 ccaggcatcg ggattgagca ggccgaagca gagcaccagg gccgcacccg cgacgaccgc
     2401 cgcggtgggg atccagcgac cccggaaggt gatcccggcg accatcacca tgacgacgac
     2461 gatcgcgacc caggcctcga agaagtcgac gagcagccgc agcgtggtgt agccgaaagc
     2521 ctgctggtag aggtgcattc gccagagggc ggatgcgacg acggcgagcg tgagaatgca
     2581 caacaggccg aggacgaccc gcagcagcaa ccggtcaccg ggatcggcct gcgggacctt
     2641 gcgcaccgtg agggcgacga ccacgagcgt cagcacggtc gcgacggtga gttggccgaa
     2701 cccctggtgg acgtagtcgg catacgtcag ccccgtcgct cgctggacga agtcgtggcc
     2761 gccgagccac gcgctggcct gggcgatgat gaacaacagg taggtcgcga cgatcagccc
     2821 gaccgggacg agccactccc agggatgggc caacggcctg cccttcggaa gggtgagggc
     2881 ttcgtcgcga atcgggttga tggccaagta cattcccgcg agcgcgaatc cgccgaagaa
     2941 gatcccgacg aagaggcggg cgacgaaggt gtcgaccgtc aggtccggga tgaggacatc
     3001 gacccaatgg ccgaagatcg catcggccga ggcgaagaga ctgccgaaga cggcgagcgc
     3061 gaggagactg atggtggcgg tccgggcgac gcgccaccac gtggtcgcag tgccactgcg
     3121 cgccagtgag cggccgagcc agggcaggcc gcggatcccg gcggtgggcc acgcggcagc
     3181 ggcccccatg agcccgtaag ccgaggagac ggggacgatc gtcacgacga tcagcagggc
     3241 cgcggccagg atcgacaggg gcacaacgac ttccgcggcc cgcagcgtag ccagcgccgc
     3301 gagacccacg ccgaggaccg cggtgatcgc ggtcatccag gtgcgtcgcc tggccgccat
     3361 cacgacggcg gcgccggtgg cgagcaggac cacactccac gcgatgccgg tgtcccgttc
     3421 gggcaggagg agcccagcca tcgcgccgac cagcgctgcc agtgcgacga ggcggggtct
     3481 gccctcgcgc acggtctctg gccacacgcg gccgaagaga ccgtcgatga ccgggccctc
     3541 ggccggacgt ggcctgctcg cgactgcccg ttcgggtcgt gcgagatcga gcgggatggg
     3601 cgcccccggt ggcctgctcg aaggcgcgcg tggcggggtc ggtggggcgg gcggcatacg
     3661 aactcctgca acggaatcgg ggcggatacg ggtcggggga tcggtgggca gcgtcaggcg
     3721 gagccgcgcg ccgcggcggc cgggctgggg gtcgatggcg gcgacggtgc cgccgtggat
     3781 ctcggcaacc cagcgggtga tggccaggcc cagcccggtg ccgcccgccg agtcggtgcc
     3841 gaatcgctcg aagatgcggt cgcggtcggc ctcggcgata ccggggcctt cgtcggcgat
     3901 gtccagatgc cagcgatcgg gtgcggccgt cgcggccgtg agcgtcaccg tggtgccgct
     3961 gggggtatgc cgcgcggcgt tgtcgagcag attggtcacc gcctggcgga gcctggcggg
     4021 ttcggcacgg actgtcagcg tcgggggccg cacgtcgatc tgggtggtga cgggccgtcc
     4081 gcccgcggca tactcggcca cgacctggtc cagaagttct tgcacggcaa cggattcgag
     4141 tcgcaggtcg gtgatgcccg cgtccagccg ggagagcgtg aggaggtcgg tcaacaggtc
     4201 gctgagccgg tcggcctgac ccagcgccga ctcgaggttt gccgggtccg caggtgtcac
     4261 gccgtccgcc atgttttcga ggagcgctcg caaagccgct gcgggagtgc gcaattcgtg
     4321 ggcgaccgtg gcgatgagct gccggcgttg ctggtcggcg tcctgcagat cggtggccat
     4381 ggcgcggaac gcgcgagcca ggtcggcgac ctcgtcgttg ccgcccacgg ggatcgcgct
     4441 cgtgtagtcg ccctgggcca tcctgcccgc agcctgggtc atggcccgca gcggtgccgt
     4501 catgccggcg gcgagccact gcgtgaccgc gagggcgacg gcgatggtca ccgggacgac
     4561 gagccacggg gtgaccttgc tgttggcccc gacggtcgcg atgacggtgg cgaagaggac
     4621 cgagacggcg accagtgcgc cgagcttgat cttcagcgag gccgcgcggg ccagtggtga
     4681 ggccatgtca gtgggactag ctgaggggct cgagggcata gccgacgccg tggacggtgc
     4741 ggatgcggtc ggcgccgatc ttggcacgca cgcctttgac atggctgtcg acggtgcgtg
     4801 tgccggacgc gtccgcccag ccccagacct cttccagcag gcgctctcgg gtcagcacgt
     4861 gaccggggct ggctgcgagc atcgccagca ggtcgaactc caacggcgtg aggtggatct
     4921 ccctgtcctg caacgtgacc cgccgtgtcg tgggatcgac cacgagatcc ccgacggtca
     4981 tcctggacgc tgggctcgcg gcgagttcgc gagcgcgttc gacgcggcgt agcagcgcct
     5041 tgacgcgggc gaccagctcg cgcatgcgga acggcttggt cagatagtcg tcggcgccca
     5101 ccccgagacc gacgaggatg tcggactcgt cgtcgcgggc ggtgagcatg atgaccggca
     5161 ccggccgctc ggtctggacc cgccggcata cctcgatgcc gtcgaatccc ggcagcatga
     5221 tgtcgagaac gatcgcgtcg gggcggtgag cggcgtggag ttcggcagcc tccggcccgg
     5281 agaaggcgga tacgacgtca tacccctccg cgcgcagccg gtcgctgacg gcctgattga
     5341 tgacgggttc gtcctccacc acgagcactg tcggcatgcc aacgacccta ggcggcgccc
     5401 cgtgacgcga tcgggttgct cttgtgtggg tttggtgcag atcggcggat gggacaatcg
     5461 tcgcgtgagt gttctggttg cgcgcatcgg caagccgcac agtctgcgcg gtgaggtcac
     5521 cgtgcagacc cacaccgacg accccgatgg gcgcttcgtc ccaggggccg tcttcgcgac
     5581 cgaggcgcag ccgggcaccg gtgtcccgag ggcgctgacg ctgcggacct cgcgtgtgca
     5641 caaaggcatc tggctgctcg ccttcgacga gattcccgac cgcacgggcg ccgaatccct
     5701 gcgcggcaca aagctgttcg ccgacgaggg cgaggccggc gacgacgcgg acgacgacgg
     5761 ctggtacgag gacgaactca tcgggctgcg cgcggtcgac cccgccggga ccgagctggg
     5821 cgaggtcgtg ggtctcgaca tcggcccagc ccaggaccgg ctgatcctgc gcctggccga
     5881 cggcaccgag gcgcaggtgc ccttcgtcga ggcgatcgtc ccggaggtcg acaccgagtc
     5941 cggccgggtc gtcatcgacc cgccgcccgg gctgctcgac ctcgggcggg agtgacatcg
     6001 tgcgcatcga tgtcgtcacg atcttccccg aataccttgc gcccctggac ctttcgctga
     6061 tcggcaaggc gcggcgcgac gatctcctcg acctcgcgat tcacgacctt cgccagttca
     6121 cccatgatcg gcacaacacg gtcgacgaca cgccgtacgg cggcggcgcc ggcatggtga
     6181 tgaagccgca gccgtgggcc gaggcgctta ctcatgtcgg tggattggct tccggcgcac
     6241 cgcgattgct cgtgcccgcc ccctcgggac gacggttcac gcaggagtat gcccgcgccc
     6301 tcgccgaggc cgagtggctc gcgttcgcgt gtggtcgcta cgaggggatc gacgagcggg
     6361 tctatacgta tgccgcggac gccctcggct gggaagtggt cccggtctcg ctgggcgact
     6421 atgtcctggg tgggggtgag gtcgccgtgc tggcgatggt cgaggcgatc gcgcggctca
     6481 tccccggcgt cgtcggcaac gaggaatccc tggtcgagga gtcccacgag ggtggactgc
     6541 tcgaatatcc cgtctacacc aaacccgcca cgtggctccc gcagccaccc gcggcggcgg
     6601 cggcgacggc atacgacgtg cccccgacct tgctgtccgg caaccacaaa gccattgccg
     6661 actggcgcca ccagcagcgc ctggagcgca ccgtcgcccg gcgcccggac ctgctcgagg
     6721 ccgctgccac ggtgacgggg ctcgaggacc tggagggcgg ccccgcgacc ccggccgaca
     6781 ttcccgaact catcgtcctc acgcgggcct gctgggtgcc ggaggccctt gacgccggca
     6841 ccgtcgactt cccgcccatg accgacggac ccgcggaaca cgctgcggca ctgcaggatt
     6901 ggtccacgtg ggtgtggcgc agcaacgggc ggctggtcgc ctccggccgc ggccggcgtg
     6961 accccgacga cccgtcggtg tggcaggtcg gccgcctcat ggtcgccccc gacctggccg
     7021 gccgcggcat cggccgcgcc gtcctcgcat tcatcgagtc cctcgcgccg gccgacgtca
     7081 cgagctattg gatcaacacc gcccgccggg ccgagcgcaa ccagcgcatc tacaagaagg
     7141 ccggctaccg gctggcgccc tgggagtcga aggtccaccc ggcgacggtg gatctggtca
     7201 agaagcgtcg ctagcggcgg ttcatcaggg ccgcagactc gcgcgcaggt cgtcgggcag
     7261 caggagggag cggcgcatca gttcctcggc cacgacagcc gtgacggtct gcgccgccat
     7321 gatgcccatg agcacgagaa cctgcgcggc agcggcctgg gccggcgagc cgccgccgag
     7381 gaggactccg atgaacgcgc cgggcagtgt gacgacgcct gcggtgcgca cttgatcgag
     7441 cccggggatc aacgcctccg ggatgtggcg atggatcacc ttgccgatgg ccgcccggcg
     7501 ctcgaaaccc agggccagcc acgcgtcata cgactcgcgt tcctcccgga gggcctggaa
     7561 ggcgcggcgg ccgacgagcg tatggccgtt catcgtgttc cccgtcagga tgccgaacac
     7621 cgggatgagg gcgatccccg tcggcgggat ggtgcgggtg agcaggatga tcgccgccac
     7681 cggcaacaac ccggcgagga tcgccaccgt cgcccacggc caggcgttcg ccgcccccac
     7741 ccggcgcacc gtcgtcacca cggcgaccgt gaacatcccc gcgagcacca gcagcgacag
     7801 cggcaggctc cccaccaccg cggtgatgac gagcgcggcg aggctcagct gcgccagcgc
     7861 gcgggcggca gcgacgagcg tggaaccgat accggggagc cggcccacgg catacgcagc
     7921 gacggtgacg gcgagcagaa gcacgatggc cagcgcgacc ggccagcccg ggttgatgct
     7981 cacctcgtca ccgtaacccc ggtcctcagc aggcgcgcca gccggcaggc tggacgcacg
     8041 gccgtctcgt cggctctgaa cccgccgcta cgcggcgccc aggcgcaccc ggccgaagtc
     8101 gcccgcgtcc agccctttct tcagcgccgg atacgcgcgc acgaaaccca gggcgcctgc
     8161 gctcatcccg agcagcgccc aaccggcgtc ccggtggccg gccccgatgc cggcgacggc
     8221 cgtgccgggg caatagccac ccagggtcat accggtgccg aagagcgctg cgccggaaag
     8281 gattccgccg agcttcacgg gtttgacgtc cggtgcgacc acgccccggc gacgcagttg
     8341 ttcgaaagca acgccaccca tggcgaccgc agtgcccaca gtgccggcga cgcgccagtc
     8401 ggtcccggag aactggttct tgatcgtcgc cgaatcgctg aggccgccgc gctgcagcag
     8461 ggcgccgaag gccagaccgg tggccagccc gagcacgttc gcgccgacac cggtctcggt
     8521 catcgccccc accgcctgcg gaccgggtcc tcccaggggc catcgagttc atacccccca
     8581 ccgtataagg gtcagcgcgc ggatggggga tcggagggca cccgtcgtca gcctcgtgga
     8641 gcgcgtcacc gggtgggtca cccgcggggg gtgactcgac gcggaggcgg gcgatttctc
     8701 ggtatgccgc ccgctctggc accattggag ggttgccatc agccttttgc gcggcccctg
     8761 ccacaggggg agtgcggttc gcacgcggcc cggcgggcgg catacgaaga actcactgac
     8821 gaaagcacgt cgctggcctg tggcagcggc gggaaagaga tgaacgccat gcagcgtttc
     8881 gacgagatcg acaaggcttc cctgaagtcc gacctgcccg ccttccgcgc gggcgacacc
     8941 gtcaaggtgc acgtgaaggt cgtcgaaggc aaccgctctc gcatccaggt cttccagggc
     9001 gtgtgcatcc gtcgccacgg cggcggcatc ggtgagacct tcaccgtccg caagatctct
     9061 ttcggcgtcg gcgtggagcg caccttcccg ctgcacaccc cgatcatcga caagatcgag
     9121 gtcgtcaccc gcggtgacgt gcgccgggcc aagctctact acctgcgcga actgcgcggc
     9181 aaggccgccc ggatccgcga gcgccgcgag accccggcca gctgaccgcc cccacgacgc
     9241 ggacccgccg agaccctgat ggccgacagt ggctgtcagg gtctcgcgtt aggctgacgc
     9301 ccctatgact tccgaggccg tcacccccga aacccgagcc gccggccgtg ccaaacgcaa
     9361 gccgttgcca gtgtgggtgc acttcctgat cgccctcgct ctggtggcgg tgctgcgcaa
     9421 tttcgtcgtg cagtccttct atgtgccgtc cgggtcgatg atcccgacgc tcgagatcgg
     9481 cgaccgcgtc gtggtctcca agctcgacaa caccttcgag cgtggcgaca tcgtcgtctt
     9541 cgacggcacc gacaccttcg gcggcggcgc cagccagcgt tgggagggaa ctttcggcaa
     9601 ggtcgcctcg gggatcgcag gcatcttcgg gatcaacctg ggcgagaagg actacgtcaa
     9661 gcggatcatc ggcgtcggcg gcgaccatct cgtcgtgggc gaggacggca cgctgacgat
     9721 caacggcaag gcggtcaacg agccttatct ggccgagggg gtcaagtcct ccgactaccc
     9781 cttcgatgtc acggtgcccg agggcaagct gttcatgatg ggcgacaacc gcgaacagtc
     9841 cgccgactcc cgcgcccacc tgggcgaccc cggcggcggc atgatccccg aatcggacgt
     9901 catcggctcg ccggtattgc gctactggcc gctgggccgg ttcggcacca tgggcgacag
     9961 cgcccacgaa ccaagcctcg ccgaactgcc ggccgggagt gcctcgtgac ctcgaccccg
    10021 ggcgcgccgc ctccgcacga cccgggtccc ggaccggagg cgcgctaccc gacccacacc
    10081 ggggtccggg cacgcgcggc cgagcctgag cccgacgccg ggtcgagccc ggccgcaccg
    10141 cggcgcagcg tcggcggcca tctcgccgcc ttggtccggg agttgctcat tgtcggcgtc
    10201 atcgccctgg tcctcagttt cctggtcaag accttcctgg tccaggcctt ctacatcccg
    10261 tccgagtcga tggaatccac gctcgtcaag gacgaccggg tgatcgtcag caagctcact
    10321 ccgagcctga tcgacctcaa acgcggcgat gtcgtcgtct tcgccgatcc cgaccactgg
    10381 ctcacgcccg agttgcgcgc cgatcgcggg ccggtgctca accgcatcca ggacctgttg
    10441 accttcgtcg gtctgctgcc cgacaccgcc gaggggcacc tgatcaagcg cgtcatcggc
    10501 ctcccgggcg acaaggtcgc ctgttgcgac accggcggcc ggctgacggt caacggcgtc
    10561 gcgatcaccg agccctacgt caagcccggt gaagagccga gcgagatcac cttcgacatc
    10621 accgtcccca gcggccgggt ctgggtgatg ggcgacaacc gcggccactc cagcgactcc
    10681 cgactgcacg acggcgacgg caagggcaac gaagggtcgg tgccgatcga cctgatcgtc
    10741 gggcgggccg tgctgaccat ctggccgctc gatcgcatca cctggctgag caacgacgcc
    10801 gacaccttcg cgaaggtccc cggcacgtga gctcggtccg gcgctccacc aaaccctccc
    10861 tgcgggtcga gcgggccctg cagcgcgacg ggtatgccgt gctggccggc atggacgagg
    10921 tcggccgggg cgcgctggcc ggcccggttt cggtgggcgt cgtcgtcatc gacgtcacct
    10981 gccgctcggc gccgcaaggg gtcaaggact ccaaactgct cacccccggc gctcgcacgg
    11041 ccctcgtgcc gcggatccac cggtggagca ctgcgtatgc cgtcggccac gcctggcccg
    11101 aggagatcga ccggatcggc atcatcgctg ccttgcgcac tgccggccgg cgcgccctcg
    11161 cccagctgcc cgtccggccc gacctggtca tcctggacgg caaccacgac tggctgaccg
    11221 acccggccga ggcggggctg ttcgcggcct ttgcgcccga tccggcgccg ccggtcacga
    11281 cgatgatcaa ggccgacctg aagtgctcct cggtggcggc cgccagcgtc ctggccaagg
    11341 tggcccgcga cgcgatgatg gtcgcctacg cccgcgagca cccggcatac ggctgggacg
    11401 agaacaaggg gtatgccgcc cccggccacc tcgccgccct cgccgagcac ggcgcctgcc
    11461 cgctccatcg ccgctcctgg cacctgccgg gtgtcggcga ggtcgtcatg ggcgatgatg
    11521 agatcgacat cgacctcctc gccggcctcg gcggggccga aagcgaaatg gcatgagcag
    11581 cgaagacctg gagaagtacg agaccgagat ggagctggcg ctctatcgcg aataccgcga
    11641 tgtcgtcggc ctcttcagct atgtcgtcga gaccgagcgg cgcttctacc tcgccaacgg
    11701 ggtcgacgtg gccgtacgcg gcgagggcag cgaggtcttc ttcgaggtca cgatgaccga
    11761 cgcgtgggtc tgggacatct accgcccggc ccgcttcgcc aagaacgtgc gcgtcatcac
    11821 cttcaaggac gtcaacgtcg aggagcttgc caaggccgat ctcgaggtgc cgaagggcca
    11881 cttctagggc cccacccgag gcacccccga aggttctccg ctgctgccgc ttgtccacag
    11941 gcggaacgtc aacttcgccc tttccacagc attttcctgc gctcctgaca cacccccctc
    12001 ccgccgggca tcgtcgtcac aacgacgacg atcaggggga cgaccatgcc ggacgcacgg
    12061 gcggaggtgg gccgcttcgg cgaggacgtg gcggcgcgct acctcagcga tcggggcctg
    12121 acgatcatcg agcgcaactg gcggtcgggc acacccaggg gagaactcga catcatcgcc
    12181 cgggcgcccg acggcgacct ggtgatcgtc gaggtcaagg cgcggcgcac cctgggcgtc
    12241 ggggccccgg tcaacggggt caccccgcag aagctgcggc aactgcgccg gctggccaac
    12301 acctggctgc gtgaacatcc ggagatcccg gcaccaggcg tgcgtttcga cgtcgtcggg
    12361 gtgctgatcg gtcgcgggag ttcaccctcg gtggtgcagc acatcccggg ggtggtcgta
    12421 tgacgctcgg agccacccac gccatcgccc tctcgggtct gcaggggcaa ctggtcgagg
    12481 tcgaggcaca tttgagcaac gccttgccgg ggttgctggt cagcgggctg ccggacgccg
    12541 cctgccggca ggcacccgat cgggtccggg cggccatcga gtccagcgat cttgcgctgc
    12601 ccagtcgcaa gatcgtcgtc aacctctcgc ccgcggccgt gcccaagaac ggcagcggct
    12661 acgacctggc gatcgccgtt gccgtcctca ccgccatcgg tgacgtcgag cagaagcacg
    12721 tctcccgagt cgtccacgtg ggcgaactcg gcctcgacgg gcgcgtgcgc ccggtcgccg
    12781 gagtgctgcc catggtgacc gccgccgctt ccgccggcat ctccgattgc attgtgccac
    12841 agacaaatct cgccgaagca tcgctcgtcg agggcatccg ggtgcatggc gtccggaccc
    12901 tcgtagacgt cgtacacgcc tacaaggagg ccgggccacc cacccgttgg ccgcggcagc
    12961 tggtgcgcgc cgtcacgggc ccggccgccc ggccggtgcc cgatctcgcg gacgtctcgg
    13021 gccaggcagc cgcccgggcc gcggtcgagg tggcggcagc cggcggtcat cacctgctgc
    13081 tcacgggccc gcccggatcc ggcaagacga tgctcgccga gcggatggtg ggcatcctcc
    13141 cgccgctgcg gctcgatcag gcactggagg tgatgtccat ccggtcgctg atcggcgcca
    13201 cgcacgccga gtccgaactc gacgtcacac caccgtatgt cgcgcctcac catggcgcct
    13261 cgctggcggc catggtcggc ggcggatcgg ggctggtgcg gccgggtgcg gtgtcggccg
    13321 cgcaccgggg cgtgctcttc ctcgacgaag cgccgcagtt caagcaggac gtcctgcagg
    13381 cgctgcgcca accgctggag tccggccggg tcgtcatcgc gcgagcccgg gccacagtgg
    13441 acttcccggc gcgcttccaa ctcgtgctgg cggccaaccc gtgtccgtgc ggccacgggc
    13501 acggaaaggc cgtgcgctgc acgtgcacac cgatggcccg ccgtgcgtac gccgcccggc
    13561 tcggcggacc gatcctcgat cgcatcgacg tccaggtcca cgtgcccccc gtgacgcggg
    13621 cggccaacac ggcggccgcg ggggaggcgt cggcggtcgt ggccgagcgg gtgcgggagg
    13681 cccgggcggc cgcggcgcaa cgctgggggc cggcctccgg tgggcggtgg ctgaccaata
    13741 gtgaggtccc cggccccgtc ctgcgagtgg gatcattccg gttgccgggg ccggtcacgg
    13801 ccgacctcga ccgggccctc gagcggggtg ccctgacgat gcgcggctac gaccggacgc
    13861 tgcgggtggc gtggacgcgc gccgacctga cggggcgttc ggtgccggtc gccgatgacg
    13921 tcgggcttgc cctcttgctc cgcgccggcg cccaggcggc ctgagatgag cattgccaac
    13981 ccctatgagc acgtgctgcg cgacgagcgt tcggcgcgga tcgcgttcgc ccggctggcc
    14041 gagcccgccg acccggcggt cgccgaggtc gtcgccaccg agggatatgc cggggcgctg
    14101 cgaggggtct tgtcggggcg gcataccaag ctcagtcgca agctcgcgcc gcgcatcggg
    14161 cacctcgacc agatcgacga cgatgcgatc gcggcggcgt tgcaggcgcg gatcgtcgtg
    14221 cccggtgacc cggcgtggcc ccgcgggctg gacgatctgg aggcggcacc gcattgcctg
    14281 tgggtgcgag gtggcgccgc ggaggcgctc gacaacctct gcacccgcag cgtcgccgtc
    14341 gtcggagcgc gggcggccac cgcctacggc ctggaccggg ccgctgatct gggcgcggag
    14401 ctgtccgagc gccggttcac cgtcgtctcc ggggcggcga tcggcatcga cgcggccgcc
    14461 catcagggcg cgctcgccgt cgaggccccg acggtagccg tcctcgcctg cggcatcgac
    14521 cggatctacc cgcgctccca cgaggtgctg ctgcagcgga tcgccgaaac gggggcgatc
    14581 gtgaccgaac tcccgccggg cgccgccccc tatcggcagc gcttcctcca acgcaatcgg
    14641 ctgatcgccg cgatgacgtg cggcaccatc gtcgtggagg cggccttgcg gtccggttcg
    14701 ctcgccactg ccgcggcggc cacgcagctc gcccgcccgg tcggggcgct gcccggcccg
    14761 gtcacctcga tgtgctccgc cgggtgccac caggcgatcc gcgagggcat tgcctcgctg
    14821 gtgaccgacg ccgccgatgt ggccgacctg ctcggcagct tcggccgcga caccgctcca
    14881 ctgcggcggg cgccggtgct gcccgaggac gaactcccag gcacccaacg ctcggtctgg
    14941 gaggtgctgc cggtgtcccg gcctgcgtcc gtggctgccc tcgccgagcg atcggcgctg
    15001 gacgagctcg ccgtccaagc cgctctcggc ccgttggaac tcgctggcct ggccagacgg
    15061 tcgggcgagc actggatgcg cgcttcgcgc gtggcgcctt gaaccgggcg gtcctttttg
    15121 cgaccgttgg cccgtggagt cggacaccgc ggtcgtggcg gcctttgcgc gtcacctcgg
    15181 cgcggagcgc ggccggtcgg cgcacaccgt gcgcgcttac gaaggtgacc tgcggtcgct
    15241 tgcggcctat ctcgacccac ccggcgcgaa cgcccgtcct tccggcggcg aaggcaaatc
    15301 ccacctgctc gccgccaccc tcaccgacct gcgtggctgg ctcggtgccc aggccgggga
    15361 gggcgtcgcg cgcgccacgc tttcccggcg tgcggcgagc gtccgcacct tcttcgattg
    15421 ggccctgcag accggtcggc tcgagaccaa ccccgccttg cggctggtct cggcgaaacg
    15481 ctcccgccgg ctgccgaccg ttctctcggc ggaggctgcc ggtgaggtgc tcgatgccgc
    15541 gggtgtcgcc gccgatgatg acgagccgtt ggcgctgcgc gaccgggcca tggtcgaact
    15601 gctgtatgcc agtggcatca gggtcggcga actcaccggt ctcgacgtcg acgacgtcga
    15661 cctcgacgcc cgggtcgcca cggtcatggg caagggtgcc cgcgaacgga cggtgccgtt
    15721 cggggccccg gccggtgccg ccctcgagcg ctggctgcgc gtcggccgtc ccgcgctcgc
    15781 cggcgcgggc agcggcccgg cgctcttcct cggccgccgc ggccgccggg tcgatcagcg
    15841 gcgggtccgc gaggtcgtcc acgccaccac ctcccgcgtc ccgggcggcg ccgagatggc
    15901 tccgcacggc atgcggcata ccgctgccac gcatctgctc gacggaggag cggacctgcg
    15961 caccgtgcag gaactcctcg gccaccgctc cctggcgacc acccagctct ac
//
LOCUS       DAOPJG010000064        15861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_3893_length_15861_cov_8.057573, whole genome shotgun sequence.
ACCESSION   DAOPJG010000064 DAOPJG010000000
VERSION     DAOPJG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 15861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15861
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3893_length_15861_cov_8.057573"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKU75_07735"
     CDS             complement(<1..439)
                     /locus_tag="PKU75_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80804.1"
                     /translation="MGFLDKAKAAAQDLQQKADTAMANSGLMGGTPMGGPVGGSGYGA
                     GRGGGEGDRLLRDLGVLTYLNAVGRPGSAEDYHRIMGQLQSLESSGGLNLSLAPAAPA
                     GYGGPGAPPPPPGAAAAAASAAAAGAAAAGAAAAGAPAGSAGAG"
     gene            561..1283
                     /locus_tag="PKU75_07740"
     CDS             561..1283
                     /locus_tag="PKU75_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HPF80805.1"
                     /translation="MTIHGSLFQDFREKETQDPFSLQNKKMLRVDMRYGEVMAKAGSM
                     VAYQGDVRFTKQSSGMGRFLKSAVTGEGMTMMTCSGQGDLFLADEAQEVQVMYLQDDA
                     ISVNGRNVLAFSNSIQWDIHRIQARGAAMTGGLYNVSLRGTGYVAITTDGDPVALDVA
                     SSPTFADAQAVVMWTNGVRMDVRVDTGGIGSMLRGGTGEMLQMAFTGQGYVLVQPSEG
                     VPQGGQEQGGGGGGMLGGILNG"
     gene            complement(1388..1882)
                     /locus_tag="PKU75_07745"
     CDS             complement(1388..1882)
                     /locus_tag="PKU75_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80806.1"
                     /translation="MSINESLKKTIDDIDLDRRVEELNQLAQKAVADVKAQLGTLAGD
                     NRGKVDEWVAKATESLDAKTDGKYHDKIQKFSVVVGQTVDKVAAQRPAGDASADRAGD
                     DAATPTAFEPEYMPSDDPWSDATMRDATPADTLADVTVTDAEAAVDGADEPGADHTGW
                     PQER"
     gene            complement(1984..2400)
                     /locus_tag="PKU75_07750"
     CDS             complement(1984..2400)
                     /locus_tag="PKU75_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80807.1"
                     /translation="MPGESHSPMFGGNSNWRGPIWFPVNVLLADALRTYAAGMASDVQ
                     VEFPHGSGEYRPVVAVADEIDQRLTGLFRMGPDGRRPSDPSDIPTDPLWQAHPTFSEY
                     FHGDTGAGLGASHQTGWTALVAHLICKPWRRHTGEL"
     gene            2921..3811
                     /locus_tag="PKU75_07755"
     CDS             2921..3811
                     /locus_tag="PKU75_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPF80808.1"
                     /translation="MQWRLGLPDRSAPTGPEPLAAPGAPATPDAQAPGSSAVASGDRM
                     PRRRAWLPWLALTLVALLAATALYRTFAIPDAYTDEQARAAASPLVEQATKEQASRPP
                     ATVAAYEKIHPSLVVIRTRGKNGLGTGFVANANGTILTAYHVIDGASTIELTFADGTK
                     TTGKVAGQEPEKDLATLTPARLPEVLVPATLGEPGPVGTPVHAAGNPLGLTFSFSSGV
                     ISALGRNIDAGSVTLKDLIQFDTAVNPGNSGGPLLDDAGRVVGVVTALANPSDQSFFV
                     GIGFAVPITAAGGSVGAAPM"
     gene            3828..4877
                     /locus_tag="PKU75_07760"
     CDS             3828..4877
                     /locus_tag="PKU75_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPF80809.1"
                     /translation="MTEPETRVDSPSSDRLTARPLEQTLYEVKKTIVGQDELLQKMIV
                     ALLARGHLLVEGVPGLAKTLAIKALAEAIGGVFQRIQFTPDLVPADVVGTRIYNQQSG
                     EFQVSLGPVFANLVLADEINRAPAKVQSALLEVMQERQVTIGRETYPSPKPFLVMATQ
                     NPIESEGTYPLPEAQVDRFMMKVKVGYPSAPEEFVVVERMMGIGEKTQKVMEPDQLAR
                     LQEAADAIYVDPGIIQYAVRLAGATRSPALVGLPDLTRLVTFGASPRASINMVLAARA
                     LAFLRGREYAIPEDARDLALDVLRHRLVLSYEALADNVDPDDIVRSIIAAVPLPDIQA
                     RVPDREQVQWQQTRT"
     gene            4856..5749
                     /locus_tag="PKU75_07765"
     CDS             4856..5749
                     /locus_tag="PKU75_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPF80810.1"
                     /translation="MAADPHLTPAALLQRIEWTVLRRLDGALQGDFATLFRGEGIDFQ
                     ELREYVPGDDLRRIDWNVTARMDTPYVRDYAEDRDLTAWLLLDRSSSMAFGPSERGKD
                     DVLVELVACFAHLFGRGGNKVGAIVFDDGPLVTIPPRSGRVQSLRLVHEVLSRQPHST
                     ATTDLAALLRAGVGVIRQRSLVIVVSDFISAPGWERPLRQLAERHDVVVVQITDPLES
                     ELPDVGIISLQDNETGEQVLVDTSDDGFRSRMRELADEQQRALTAAVRSAGITLHTVS
                     TAEDLGGAFVRMATRRKLARR"
     gene            5746..6738
                     /locus_tag="PKU75_07770"
     CDS             5746..6738
                     /locus_tag="PKU75_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF80811.1"
                     /translation="MTFEIPLALCALIVLPLLVLTYRSQWGRARRGREALARTGLRQV
                     GSVETSRWRHVAPGLLLASLGTLIVAAAAPASVLTITKPEGTVILAFDVSNSMRAEDL
                     EPDRLTAAKTAAKAFVAKQPSSVRIGLVAFSDTGIVTQQPSDDPATVSQAIDRMAPHG
                     GTSLGQGIFAALQAITDGRLRADPEKLAEDPESIDIGYHGEASIILLSDGQDQSDVDP
                     LAMARLASVAGVRIDTVGLGSGEGTEVTVNGVTQQTALDAAVLTEIAKTADGVYHPAQ
                     DAAALTEVYDSIELVDKPRRELTGLAPYFMIAAALLAAAAMGLSLVRSGRVIAS"
     gene            6735..7829
                     /locus_tag="PKU75_07775"
     CDS             6735..7829
                     /locus_tag="PKU75_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF80812.1"
                     /translation="MTFTWPAALMLLTLVPILLILYVWAQRRRRAAAIQHSNVSLVRA
                     AVGARRRHWTRHLPLAAMLLGLTALGVGAARPTMAMTVPQNRTSIIVALDVSRSMCSV
                     DVDPNRFSAAKKALREFVAAQDPDTRIGLIAFAGYAEMVSPPSTDRGQLEATLEALPT
                     GRGTAIGAAILRSVDAIASINPKVAPVGPDEAPEPPEEQQFPLPAPDLSTVKPPTTEP
                     IADVVVLLTDGANTRGVLPMDAAAVAAKRGIRVYPIGFGTTQPTQMVCTRDQLGADAY
                     SEGGAYGRGNAAQFLVVDEPTLEGVAKATGATYSPATNSAELSESLADVPKNIVLTTE
                     KVELSPAFAAGGALLLLLGVLIASRQRVFP"
     gene            7932..8162
                     /locus_tag="PKU75_07780"
     CDS             7932..8162
                     /locus_tag="PKU75_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HPF80813.1"
                     /translation="MTIRNLSDATHNALKARAKRNGRSAEAEVRAIIDAAVAPAEARG
                     LGSLLAEIGAQAGGIELEIERDRTAREPLDFA"
     gene            8159..8578
                     /locus_tag="PKU75_07785"
     CDS             8159..8578
                     /locus_tag="PKU75_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPF80814.1"
                     /translation="MILLDTNVVSEPLRPQPDSRAVPWLDAQVHETLYLSTITIAEMR
                     FGIAALPAGRRRRILSERFEGEVMPLFHGRILAFDELAATAYARIRARVRQRGRALGD
                     FDALIAAIADANGLTVASRDTGPFVVAGVPVINPFTP"
     gene            complement(8568..8948)
                     /locus_tag="PKU75_07790"
     CDS             complement(8568..8948)
                     /locus_tag="PKU75_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80815.1"
                     /translation="MPKKIDPAVKERCVRQVLEHLPEYPSLTAREENRGPAEDLVIFL
                     EPPDLGLELLDLSQLLGRGALALPAVDLGLDHPATHGLLPEPLAASDGLRGEHQSRPV
                     ESAPLFTACRALSTGMRGWGGVRA"
     gene            complement(9031..10494)
                     /gene="hflX"
                     /locus_tag="PKU75_07795"
     CDS             complement(9031..10494)
                     /gene="hflX"
                     /locus_tag="PKU75_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPF80816.1"
                     /translation="MTEPLDDLRGDDADDILARPAEALAGTTYDHDDRDGDQFDREER
                     AALQRVEGLSTELEDITEVEYRQLRLERVVLAGVWTEGAAEDAENSLRELAALAETAG
                     STVLAGLMQRRQRPDPGTWLGSGKAQELRDVVIAEGADTVVCDSELAPSQRRALEDIV
                     KVKVIDRTALILDIFAQHAKSREGKAQVELAQLQYLLPRLRGWGESMSRQAGGRVAGG
                     EGIGSRGPGETKIELDRRRINSRIAKLRRDIRDMKTHRDTKRHSRKAHGTPSVAIAGY
                     TNAGKSSILNRLTGAGVLVENQLFATLDTTVRRAETPDGREFTLTDTVGFVRQLPHEL
                     IDAFRSTLEEVADADLLLHVVDGSHPDPEGQISAVRAVLADVDAADVKEVIVVNKADI
                     ADPEVVDRILRHEKHAIAVSAHTGAGIAELIELIADELPRPSVRVVATVPYARGDLMS
                     RIHESGEIAETEHREDGTYVVALVGPDLAADLAAFAS"
     gene            10584..11186
                     /locus_tag="PKU75_07800"
     CDS             10584..11186
                     /locus_tag="PKU75_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80817.1"
                     /translation="MTVLPRRVVIGAAIAAPASLLTGCQKEDTRAQAVLSKVDQIVIA
                     DVLDESTATGMTVRPTSIIWSAGEAWAGDELFPIPDLPLAETRVYPGRSADRPETGER
                     AMLLLAHPAPDWKIQLVAEPDTAAIAGTSTAADNAREAVAAGMTIRGADADQLAVLIE
                     LIEGARGIEDAENGHGDPAGAGGLMKEVLPHLSQEAGGPG"
     gene            complement(11193..11612)
                     /locus_tag="PKU75_07805"
     CDS             complement(11193..11612)
                     /locus_tag="PKU75_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPF80818.1"
                     /translation="MNYRTVVARVVAYCASSGLAALVDLTAFAVIIDAGFGGRSYLAA
                     VATATVGARAISSAVNYAVNRHFVFVRPGSTLRYFALVAAILAASSLCTTGMSALLAG
                     HVVWAKVLVDGVLFVIGYQVQRRWVFGREQEGCGTRA"
     gene            complement(11671..13374)
                     /locus_tag="PKU75_07810"
     CDS             complement(11671..13374)
                     /locus_tag="PKU75_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80819.1"
                     /translation="MAQILPIAGALLLGVAQLGFIGALRRTARVPTAVAPAVAVALQL
                     ILAFACGLAGALSLAPFVIVGVGGFLLGIEVRRGRQALLGHVIDPSVVVLAVLTAALV
                     WILPGARLLHYDNFSHWGLVVRFMLRQDRFPTATDTIVGFHTYPLGTASWEYAVARLL
                     GGREWQMMLAQGYILATACVSILALTPRRRWAGLVGVAALVVAFLLHGPRLDTLLVDN
                     VLAAWTIVGLVTISACRGNVGRAAFPLAVLCAALVATKQTGVFWVIVLCLTGLVLRAA
                     SPWQRALSLLAPALGAGLTWAAWRRHTATTFAGLPDSKHSFSREQVREMLAEKTPEVR
                     AQIHDLFLTAVRGDQLLWLALAGLALALAVAGRVRHRPRRAAVAMGGAALVSVLFLGG
                     QYAVYVLSMPAREALQLASFDRYLSTLHLVLAAAALIVGAHLAEQARPSRSAGAFVMG
                     LGGVVSLLITSWPRPLWSPVDGTLRDRVESAFATVSVPPGSEICLYGDHPSGYLRYLV
                     RYVSLADDVVSTPVPDRAGWPIPPACDYVAVVDRSPRAAAYLRASGYPVTDTVPVVMQ
                     R"
     assembly_gap    13650..13749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13750..13962)
                     /locus_tag="PKU75_07815"
     CDS             complement(<13750..13962)
                     /locus_tag="PKU75_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPF80820.1"
                     /translation="MTQAKNDRVLGTLHPDGDSGSVRVQDTYDTGMDDLWSALTEPDR
                     LARWIGTVDGELRLGGEFRAAFFTSGW"
     gene            complement(13959..14285)
                     /locus_tag="PKU75_07820"
     CDS             complement(13959..14285)
                     /locus_tag="PKU75_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPF80821.1"
                     /translation="MEAALRALADESRRTMLEALAERPRTVTELADLLPIARPGVSRH
                     LRVLREAGLVTVEADAQRRIYRLQPDPLTEVEDWLTTYRARWEQRLDALHTEIARGTR
                     DRRSTP"
     gene            complement(14407..>15861)
                     /locus_tag="PKU75_07825"
     CDS             complement(14407..>15861)
                     /locus_tag="PKU75_07825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPF80822.1"
                     /translation="GAVISMNPITAPILYPLSHRGWERPYVTAGLTLYDTMGGARSVP
                     GHKQLSRNGALRLVPALRKDALTGALLYYDGQMDDARHTLTVARTAAAYGATVLNSAQ
                     VVELTRAGERITGAVVEDIETGARTTVKASVVVNCTGVWTDAIQTLAGGRGRFNVRAS
                     KGVHIVIARDRINSEVGLILRTETSVLFVLPWGTHWIIGTTDTDWTFDLAHPAASQAD
                     IDYLLEQVNSVLTHPVTHADIQGVYAGLRPLLSGEDEQTSQLSREHAVARPQPGLISI
                     AGGKYTTYRVMAADAIDAAREDIGPGVADSVTEHIPLLGADGYPALMNQIDRLSRKVD
                     LPVWRLTHLLGRYGSLVDEVFELAEGDPTLYEAVPGAEEYLKVEILYGVTHEGALHLD
                     DLLARRTRASIETLSRGVDSAQAVAELVAPVLGWDEARTAMEVQLYAQRVMAELESQQ
                     MSDDREANAERTSARDARLNITAMVPDPDPELSD"
BASE COUNT         2431 a         5425 c         5488 g         2417 t
ORIGIN      
        1 tacccgctcc ggcggagccg gcaggggcac cagcagcagc ggcaccagcg gcagcggcac
       61 cagcggcagc ggcgctcgcg gcggccgcag cggcaccggg cgggggcggc ggggcgcccg
      121 ggccgccata cccggccggc gccgcaggcg cgagcgagag gttgagcccg ccggaggact
      181 ccagcgactg caactgaccc atgatccggt ggtagtcctc ggccgatccc ggccggccca
      241 cggcgttgag ataggtcagc acgccgaggt cacgcagcag ccgatcgcct tctcccccac
      301 cgcgacccgc gccgtacccc gatccgccga ccggcccgcc catcggcgtg ccacccatca
      361 gtcccgagtt cgccatcgcc gtgtcggcct tctgctgcag atcttgcgcg gccgccttgg
      421 ccttgtcgag gaaccccatc gccttctcct tcgtcggaca caccggattt gcccaaacca
      481 tattcggcgg cgccgcccgg cgctaccgtt tgcgcaggcg ctcgcgaacc gttcgcgagc
      541 cgacatacag gggatgaaag atgaccatcc acgggtcgct gttccaggac ttccgcgaga
      601 aggagacgca ggatcccttc tcgttgcaga acaagaagat gctgcgcgtc gacatgcgct
      661 acggagaggt gatggccaag gccggctcga tggtcgccta tcaaggcgac gtccgcttca
      721 ccaagcagag ctcgggcatg gggcgcttcc tgaagtccgc cgtcaccggc gagggcatga
      781 cgatgatgac ctgctccggg cagggcgacc tgttcctcgc cgacgaggcg caggaggtgc
      841 aggtcatgta tctgcaggac gacgcgatct ccgtcaacgg ccgcaatgtg ctcgccttca
      901 gcaactccat ccagtgggac atccaccgga tccaggcccg cggcgccgcc atgaccggtg
      961 ggctctacaa cgtctccctg cgcggcaccg gctatgtcgc gatcaccacc gatggcgatc
     1021 cggtggcatt ggacgtcgcc tcctccccca cgttcgccga cgcccaggcg gtcgtcatgt
     1081 ggaccaacgg ggtgcggatg gacgtgcggg tggacaccgg cggcatcggc tcgatgctgc
     1141 gcggcggcac gggcgagatg ctgcagatgg ccttcaccgg ccagggctat gtgctggtcc
     1201 agccgagcga gggcgtgccc caaggcggtc aggagcaggg cggcggcggt ggcggcatgc
     1261 tggggggcat cctcaacggc tgacgatcgt cacgcggcac gtcgaaagag ccagcacgtc
     1321 gaaagagcgc gacacgccca tgatcagacc cgatcatggg cgtgtcgcgc tctctcgatg
     1381 tgtttgctca gcgctcctgt ggccacccgg tgtggtcggc acccggttcg tcggcgccgt
     1441 cgacggcggc ctcggcgtcg gtcaccgtga cgtccgcgag cgtgtctgcc ggggtcgcgt
     1501 cgcgcatcgt ggcgtccgac caggggtcgt cgctgggcat gtactccggc tcgaaagccg
     1561 ttggcgtcgc agcgtcgtcc ccggcacggt ccgcgctcgc gtcgccggcc gggcgctggg
     1621 cggccacctt gtcgacggtc tggccgacga ccacgctgaa cttctggatc ttgtcgtgat
     1681 acttgccgtc ggtcttggcg tcgagcgatt cggtggcctt ggcgacccac tcgtcgacct
     1741 tgccccggtt gtcgcccgcg agcgtgccga gttgcgcctt gacgtcggcg acggccttct
     1801 gggcgagctg gttcagctcc tcgacgcgcc ggtcgaggtc gatgtcgtcg atggtcttct
     1861 tgagcgactc gttgatggac atggtgtgaa tcccctcctg cgtgggatcg cgtggccctc
     1921 ggaatccgga ccctcgatgc tggtcaacac caccttgtca cggcttgttc cgggacacgc
     1981 tggtcacagt tcaccggtat gccgccgcca cggcttgcag atcaggtgcg ccaccagcgc
     2041 ggtccagccg gtctggtggg aggcgccgag gccggcgccg gtgtcaccgt ggaagtactc
     2101 gctgaaggtc gggtgggcct gccacagcgg gtcggtgggg atgtcgctcg ggtcgctcgg
     2161 gcggcgcccg tcgggaccca tccggaacag gcccgtcagc cgctggtcga tctcgtcggc
     2221 gaccgcgacg accgggcgat actcgcccga cccgtgcggg aactcgacct ggacatcgga
     2281 ggccatcccc gcggcatacg tccgtagagc gtcggccagc aggacgttga cggggaacca
     2341 gatcggcccg cgccagttgg agttgccgcc gaacatcggc gagtgcgact cgcccggcac
     2401 ctaggcgatg ggcaggtcgc cagtactcct tgacgtcctc gccgtggttg ccctgcgggt
     2461 tggtcaggcc gaagagccgc tccttgagcc ggtcgtcgtg gcaggtcttc gcgatcgagg
     2521 gcgcgaacga ccgggccttc ctggtgcgag cccggcgatg ccgtcatcgc cccaccggtc
     2581 ggcgcgggtg tgggcgtgat cgaacgggaa ggacgcccag gcgtcgccgt cggcgaagta
     2641 gtcctcgcgc accgtccccc actgccgggc cgcgagatag ggcccccaca accgccacgg
     2701 cgcatcggcg ccgcgggact ccgccaagcg cgcatgctcc ggtgtcggac tgggctgtgg
     2761 cgtcttcgac gactcgcgtg cggcctcggt cggacgttcc ggtacggcct gggtcatggg
     2821 tgacaccatg ccaagggtcg cgccccatgg aagctgtgaa gtcgatgtga gttcaacttc
     2881 caggaggcgc gcggccggaa gcgccaatta cagtcccacc atgcagtggc gactcggcct
     2941 gccggaccgg agcgcgccca cggggccgga gcccctggcc gcgccaggtg cgccagcgac
     3001 cccggacgcc caggcgcccg gttcctcggc cgtcgcctcg ggggatcgta tgccgcgccg
     3061 gcgcgcctgg ctgccgtggc tcgccctcac gcttgtcgcc ctgctcgccg ccacggcgct
     3121 gtatcgcacc ttcgcgatcc cggacgccta caccgacgag caggcgcggg ccgccgccag
     3181 cccgctcgtc gagcaggcca cgaaggaaca ggcgagccgg ccgccggcga cggtcgcggc
     3241 atacgaaaag atccatccct ccctcgtggt catccgcacc cgcggcaaga acggcctggg
     3301 caccggcttc gtcgccaatg ccaacgggac gatcctgacg gcataccacg tcatcgacgg
     3361 cgcctccacg atcgagctga ccttcgccga cggcaccaag acgaccggga aggtggccgg
     3421 ccaggagccg gaaaaggacc tggcgacctt gacccccgcc cggctgcccg aggtcctcgt
     3481 gcccgccacc ttgggcgagc ccgggccggt cggcaccccg gtgcatgccg ccggcaatcc
     3541 gctgggcctg accttctcct tctcctccgg cgtcatctcg gccctcggcc gcaatatcga
     3601 cgccggttcg gtgaccctga aggacctgat ccagttcgac accgccgtca accccggcaa
     3661 ctcgggcggg cctctcctcg atgacgcggg ccgggtcgtc ggtgtcgtca ccgcgctcgc
     3721 caacccctct gaccaatcgt tcttcgtcgg catcggattc gccgtgccga tcacggccgc
     3781 cggcggctcc gtcggcgcgg ccccgatgtg acccgaccga agccgacatg acagaaccgg
     3841 agacccgcgt ggacagtccc tcgagcgacc gcctcaccgc ccgcccgctg gaacagaccc
     3901 tctatgaggt caagaagacg atcgtcgggc aggacgagtt gttgcagaag atgatcgttg
     3961 cgctgctggc gcgcgggcac ctgctcgtcg aaggtgtccc gggcctggcc aagacgctgg
     4021 cgatcaaggc gctcgccgag gcgatcggcg gagtcttcca gcgtatccag ttcaccccag
     4081 acctcgtgcc ggccgatgtc gtcggcacgc ggatctacaa ccagcagagc ggggagttcc
     4141 aggtctcgct cggcccggtc ttcgccaatc tcgtcctcgc cgacgagatc aaccgggccc
     4201 cggccaaggt gcagtccgcg ttgctggagg tcatgcagga gcggcaggtg acgatcggcc
     4261 gcgagaccta tccgtcgccc aagccgttcc tcgtcatggc gacccagaac cccatcgagt
     4321 ccgagggcac ctacccgttg ccggaggcgc aggtcgaccg gttcatgatg aaggtcaagg
     4381 tcggatatcc ctctgcccct gaggagttcg tcgtcgtcga gcggatgatg ggcatcggcg
     4441 agaagacgca gaaggtgatg gagcccgacc agctggcgcg gctgcaggag gccgccgacg
     4501 cgatctatgt cgacccgggg atcatccagt atgcggtgcg cctcgccggc gcgacgcgca
     4561 gcccggctct cgtcgggctg cccgatctga cccggctggt gactttcggt gccagtcccc
     4621 gtgcgtcgat caatatggtg ctcgccgcgc gggcgttggc gttcctgcgc gggcgcgagt
     4681 acgcgatccc ggaggatgcc cgcgacctgg cgctcgatgt gctgcgccac cggctcgtgc
     4741 tgtcctatga ggcgctggcc gacaatgtcg atccggacga catcgtgcgt tcgatcatcg
     4801 ccgccgtccc cctgccggac atccaggccc gcgttcccga tcgtgagcag gtgcaatggc
     4861 agcagacccg cacctgactc ccgctgccct gctgcagcgg atcgagtgga ccgtcctgcg
     4921 ccgcctcgac ggggcgttgc agggcgactt cgccacgctc tttcgcggtg agggcatcga
     4981 tttccaggag ttgcgggaat acgtccccgg tgacgacctg cgccggatcg actggaatgt
     5041 cacggcccgg atggacacac cgtatgtccg tgactatgcc gaggatcgcg atctgacggc
     5101 ctggctgctc ctcgaccggt cctcgtccat ggcgttcggt ccctcggagc ggggcaagga
     5161 cgacgtgctg gtcgagctcg tggcctgctt cgcgcacctt ttcgggcgcg gcggcaacaa
     5221 ggtgggtgcg atcgtgttcg acgacggccc gttggtgacg atcccgccgc gcagcgggcg
     5281 cgtgcagtcg ctgcggctgg ttcacgaggt gctctctcgg cagccgcatt cgacggccac
     5341 gacggatctc gcggcgctct tgcgtgccgg cgtgggggtc atccggcagc gttcgctcgt
     5401 catcgtcgtc tccgacttca tctcagcgcc cgggtgggag cgtccgctgc gccagctcgc
     5461 cgagcggcac gatgtcgtcg tcgtccagat caccgatcca ctcgagtcgg agttgcccga
     5521 cgtggggatc atttcgttgc aggacaacga gaccggcgaa caggtgctcg tcgacaccag
     5581 cgacgacggg ttccggtccc ggatgcgcga actcgccgac gagcagcagc gagcgctcac
     5641 cgccgcggtc cgctcggcgg ggatcacgct gcataccgtc tccactgcgg aggacctcgg
     5701 cggggcgttc gtgcggatgg cgacgagacg gaagctggct cggcgatgac cttcgagatc
     5761 ccgctcgcgc tgtgcgccct gatcgtcctg ccgctgctgg tactgaccta tcgctcccaa
     5821 tggggtcggg cccgacgggg ccgggaagcg ttggcccgca ccgggcttcg ccaggtcggg
     5881 agcgtcgaga ccagccgctg gcgacacgtc gcccccggcc tgctgctcgc ctcgctcggc
     5941 actctcatcg tcgcggctgc ggcaccggcg tccgtgctga cgatcaccaa accggaaggc
     6001 acggtgatcc tcgccttcga cgtctccaac agcatgcgcg ccgaggacct cgaaccggac
     6061 cggctgacag cggccaagac agcggccaag gccttcgtcg cgaagcaacc gtcgtcggtg
     6121 cggatcggcc ttgtcgcctt ctccgacacc ggaatcgtca cccagcagcc gagcgacgac
     6181 ccggcgacgg tgagccaggc gatcgaccgg atggccccgc acggtggcac ctctttgggg
     6241 cagggcatct tcgccgcgct gcaggcgatc accgacggcc ggctgcgggc cgatcccgag
     6301 aagctggccg aggatcccga gagcatcgac atcggctatc acggcgaggc ctcgatcatc
     6361 ctgctctcgg acgggcagga ccagtcggat gtcgatccgc tcgccatggc ccggctcgcg
     6421 tcggttgccg gcgtgcggat cgacacggtc ggcctgggca gcggagaagg caccgaggtg
     6481 acggtcaacg gggtgactca gcagaccgcg ctcgacgcgg ccgtgttgac cgagatcgcc
     6541 aagacggcgg acggggtcta tcacccggcc caggatgccg ctgccttgac cgaggtctac
     6601 gacagcatcg agctggtcga caagccacgc cgtgaattga ccggtctcgc accgtatttc
     6661 atgatcgccg ccgccttgct ggcggcagcg gcgatggggt tgtcgctcgt gcgttccggg
     6721 cgggtgatcg cctcatgacg ttcacgtggc ctgcggcgct gatgctcctc acgctcgttc
     6781 ccatcctgct catcctctat gtctgggccc aacgccggcg ccgcgccgcg gccattcagc
     6841 actccaatgt gtcgctcgtg cgggcggcgg tcggcgcgcg gcgacgtcac tggacgcgac
     6901 atctgccgct ggcggcgatg ctgctcggat tgacggcgct gggcgtcggg gcggcccgcc
     6961 cgacgatggc gatgacggtg ccacagaacc ggacctcgat catcgtcgcg ctcgatgtgt
     7021 cccgctcgat gtgctcggtc gacgtcgacc cgaaccggtt cagcgcggcc aagaaggcac
     7081 tgcgcgagtt cgtggcggcc caggatccgg acacccgcat cgggctgatc gccttcgcgg
     7141 gctatgccga aatggtgtca ccgccgtcca cggaccgcgg ccagctcgag gcgaccctgg
     7201 aggcgctgcc gaccggacgt ggcacggcca tcggcgcggc catcctgcgt tcggtcgacg
     7261 cgatcgccag catcaacccc aaggtcgccc cggtgggccc cgacgaggcg cccgagccgc
     7321 cggaggagca gcagttcccg ttgcccgcac ccgacctgag caccgtcaag ccgccgacga
     7381 ccgaacccat cgccgacgtc gtcgtcctct tgacggacgg cgcgaacacc cgcggggtgc
     7441 tgccgatgga tgccgctgcc gtggcggcga aacgcggaat ccgggtctac cccatcggtt
     7501 tcggcaccac gcagcctacc cagatggtgt gcacccgcga tcagctcggc gccgacgcct
     7561 actccgaggg cggcgcatac ggccgcggca acgccgccca gttcctcgtc gtcgacgagc
     7621 cgacgctcga gggtgtggcc aaggcgaccg gcgcgacata ctccccggcg accaacagcg
     7681 cggagttgtc cgagagtctc gccgatgtcc ccaagaacat cgtcctgacg accgagaagg
     7741 tcgaactctc ccccgccttc gccgccggcg gcgccctgtt gctcctcctc ggggtcttga
     7801 tcgcctcccg gcaacgcgtc ttcccctgac cgcggccatc ccgccccttt acggcaagac
     7861 gtgcctttcg atggcgagtg atatcatcga ctcttgattg atatcaagat cctggggttc
     7921 cgatgccggc catgaccatt cgcaatctct cggacgccac acacaacgcc ctgaaggcgc
     7981 gggccaagcg caacggccgg agtgcggaag ccgaagtccg agcgatcatc gatgctgcgg
     8041 tcgctcccgc tgaggcgcgt ggattgggct ccttgctcgc ggagatcggc gcgcaggccg
     8101 gaggcatcga gttggagatc gagcgcgacc ggacggcgcg cgagccgctc gacttcgcat
     8161 gattctgctc gacaccaatg tcgtctccga accactacgg ccacaacctg attcgcgggc
     8221 ggtgccgtgg ctggacgcac aagtccacga gaccctctac ctctcgacga tcaccatcgc
     8281 ggagatgcgg ttcggaatcg ccgccctccc ggcgggcagg cgccggcgaa tcctgagcga
     8341 gcgcttcgag ggcgaggtca tgccgttgtt tcacggccgg atcctcgcgt tcgacgaact
     8401 ggccgccacg gcttacgccc gcatccgtgc acgcgttcgg cagcgcggcc gcgccctcgg
     8461 cgacttcgac gctctcatcg ccgccatcgc ggacgcgaac ggcctcaccg tggcgtcccg
     8521 ggacaccggg ccgttcgtcg tcgccggcgt cccggtcatc aaccctttca cgccctgacc
     8581 ccgccccacc ccctcatccc cgtcgagagc gcacgacacg ccgtaaagag cggggcggat
     8641 tcaactggtc gagactgatg ctcgccgcgg agtccgtcgc tcgccgcgag gggctcggga
     8701 aggagaccgt gcgtcgctgg gtggtccagg cccaggtcga cggcgggcag cgccagggcg
     8761 ccacgtccga ggagctggct gagatcaagg agctcaaggc caaggtccgg cggctcgagg
     8821 aagataacga gatcctccgc cgggcctcga ttttcttcgc gcgcggtcag cgacgggtac
     8881 tccggcaggt gctccagcac ctgccgcacg caccgctcct tgaccgcggg atcgatcttc
     8941 ttcggcatgg ttcgcatcct tccaactcaa aaggatgcgg catcaaacct ggggcgcttc
     9001 cctctcgacg gtggcgtggt ggtgggttcg tcaggaggcg aaggcggcca agtcggcggc
     9061 gagatcgggg ccgacgaggg ccacgacata tgtcccgtcc tcgcggtgct cggtctcggc
     9121 gatctcgccc gactcgtgga tccgagacat caggtcgcca cgggcatacg ggacagtggc
     9181 gacgacgcgc acggacgggc ggggcagttc gtcggcgatc agctcgatga gttcggcgat
     9241 gccggcgccc gtgtgagcag agacggcgat ggcgtgcttc tcgtgccgca ggatccggtc
     9301 gacgacctcg ggatcggcga tatcggcctt gttgacgacg atgacctcct tgacatccgc
     9361 cgcgtccaca tcagcgagaa ccgcacgcac cgcgctgatc tgcccctcgg ggtcggggtg
     9421 ggacccgtcg acgacgtgca gcagcaggtc ggcatcggcc acctcctcca aagtggagcg
     9481 gaaggcgtcg atcaactcgt gcggcagctg ccggacgaac ccgacggtgt cggtgagggt
     9541 gaattcgcgg ccgtccgggg tctcggcgcg gcggacggtg gtgtcgaggg tggcgaagag
     9601 ctggttctcg accagaacgc ccgcgccggt gaggcggttc aggatcgagg acttgccggc
     9661 gttggtgtat ccggcgatgg cgacgctcgg cgtgccgtgc gccttgcggg agtggcgctt
     9721 ggtgtcgcgg tgggtcttca tgtcgcggat atccctgcgc aacttcgcga ttcgcgagtt
     9781 gatgcggcgc cggtcgagct cgatcttcgt ctcaccaggg ccgcgggagc cgatgccctc
     9841 accgccggcg acccggccac cggcctgacg tgacatcgac tcaccccagc cacgcaggcg
     9901 cggcaggaga tattgcagct gggccagctc gacctgcgcc ttgccctcgc gggacttggc
     9961 gtgctgggcg aagatgtcga ggatcagcgc ggtccggtcg atgaccttga ccttgacgat
    10021 gtcctcgagc gcgcggcgct gggacggcgc cagttcggag tcgcacacga ccgtgtcggc
    10081 gccttcggcg atgacaacgt cgcgcaattc ctgtgccttg ccggagccga gccaggtgcc
    10141 gggatcgggg cgctgccgac gctgcatcag ccccgcgagc acggtcgatc cggccgtctc
    10201 ggcgagcgcg gccaactcgc gcagggagtt ctcggcgtct tccgcggcgc cctcggtcca
    10261 gactccggcg aggacgaccc gttcgagccg cagctggcgg tactcgacct cggtgatgtc
    10321 ctcgagctcc gtcgagagtc cttcgacgcg ctgcagcgcc gcgcgctcct cgcgatcgaa
    10381 ctgatcgccg tcgcggtcgt cgtggtcgta tgtcgtgccg gccagcgcct ccgcagggcg
    10441 ggccaggatg tcgtcggcgt cgtcgccgcg cagatcgtca agaggttcag tcatcgttct
    10501 cccaggttct cacgatgcca acgcgctgag ccacctggtt attcgggcac tcgtgatccg
    10561 atccggttgc ctaccgttgt cgtatgacag tgctgccgcg ccgcgtcgtg atcggcgccg
    10621 ccatcgcggc gcctgcttcg ctgctcaccg gctgccagaa agaggacacg cgcgcccagg
    10681 ccgtgttgag caaggtcgac cagatcgtca tcgccgacgt cctcgacgag tcgaccgcga
    10741 ccgggatgac cgtgcggccc acgagcatca tctggagcgc cggcgaggcg tgggccggcg
    10801 acgaactctt ccccatcccc gatctcccgc tcgccgagac gcgggtgtat cccggccggt
    10861 ccgcagacag accggaaacc ggcgagcggg ccatgctgct gcttgcccac cccgcacccg
    10921 actggaagat ccagctcgtc gccgaacctg acaccgcggc gatagcgggc acgagcacgg
    10981 cggccgacaa cgcccgcgag gccgtggccg cgggcatgac gatccgaggc gctgacgccg
    11041 accagctcgc cgtcttgatc gaactgatcg aaggcgcccg agggatcgag gacgccgaga
    11101 acggacacgg cgatccggcc ggcgcaggcg gcctgatgaa ggaggtcctc ccccacttgt
    11161 cgcaggaggc gggcggcccc ggctgacgct ccctaggcac gcgtgccgca gccctcctgc
    11221 tcgcgcccga acacccagcg ccgctggacc tgatagccga tcacgaagag cacgccgtcg
    11281 acgagcacct tcgcccacac gacgtgcccc gccaacagcg cggacatgcc ggtcgtacac
    11341 aggctggagg ccgccaggat cgccgccacg agcgcgaagt agcgcagcgt cgatccggga
    11401 cggacgaaca cgaaatgccg gttgacggcg tagttgacgg cggaggagat ggctcgggcg
    11461 cctactgtcg cggtcgccac ggcggcgagg taggagcggc cgccgaagcc ggcgtcgatg
    11521 atcacggcga aagcggtcag gtcgaccagg gcggccaatc cggacgaggc gcagtaggcg
    11581 acgacgcgcg cgacgacggt gcggtagttg atgatcgagg gtacgtcgcg ctcgcgcggc
    11641 tcgacgacgt ggagcgaccg ggcgggttgg tcatcgctgc atcaccaccg gcacggtgtc
    11701 agtgacggga tatcccgacg cccgcaggta tgccgcggcc cgcggcgaac ggtccacgac
    11761 ggcgacgtag tcgcaagcgg gagggatcgg ccagccggcg cgatccggca cgggcgtgga
    11821 cacgacatcg tccgccagac tcacgtagcg cacgaggtaa cgcaggtatc cgctcgggtg
    11881 gtcgccgtag aggcagatct cggagccggg tggcaccgac acggtggcga aggcgctctc
    11941 cacccggtcc cggagggtcc cgtcgaccgg gctccacagc ggccgcggcc aggaggtgat
    12001 gagaagggag accacaccgc ccagtcccat cacgaatgcg cccgccgacc gggacggtct
    12061 cgcctgctcg gcgagatgcg ccccgacgat cagcgcagcc gccgccagca cgaggtgcag
    12121 tgtgctcaga tagcggtcga agcttgccag ctgcaaggcc tcccgcgccg gcatggacag
    12181 cacgtagacg gcatactgcc cgccgaggaa gagcaccgac accagcgccg ccccacccat
    12241 cgccaccgcc gcgcgacgcg gccggtgccg gacgcgtccg gcgacggcca gcgcgagggc
    12301 gagcccggcg agagcgagcc acagcagctg atcgccgcgg acagcggtga ggaagaggtc
    12361 gtggatctgc gcgcgcacct cgggagtctt ctccgcgagc atctcgcgca cttgctccct
    12421 cgagaaggag tgtttgctgt ccggcaggcc cgcgaacgtg gtcgccgtat gccgccgcca
    12481 cgccgcccag gtcagcccgg caccgagcgc gggagcgagc agcgacaggg cgcgctgcca
    12541 cggtgaggcg gcccgcagca ccagacccgt caggcacagg acgatcaccc agaagacgcc
    12601 ggtctgcttg gtcgcgacga gcgccgcaca cagcaccgcg agagggaacg ccgcccggcc
    12661 gacgttgccg cgacatgctg aaatcgtcac cagcccgacg atggtccagg cggcaagcac
    12721 attgtcgacg agcagggtgt ccaggcgcgg gccgtgcagg aggaaggcaa cgacgagtgc
    12781 ggccaccccg accaatcccg cccaccggcg acgcggcgtg agggcgagga tgctgacgca
    12841 cgcggtcgcc aggatgtagc cctgggcgag catcatctgc cactctcggc caccgaggag
    12901 ccgagccacg gcatactccc agctcgctgt cccgagcggg taggtgtgga agcccacgat
    12961 cgtgtccgtg gcggtgggga aacggtcctg acgcagcatg aaccggacga ccaggcccca
    13021 gtgactgaag ttgtcgtagt gcagcaggcg ggcgccgggc aggatccata cgagagcggc
    13081 ggtgagcacg gcaaggacga ccaccgacgg atcgatcacg tgtccgagca gagcctggcg
    13141 cccacgccgg acctcgatcc cgagcaggaa accacccaca ccgacgatga cgaagggggc
    13201 gagcgacaag gcaccggcca gtccgcatgc gaaggccagg atcagttgca gggccacggc
    13261 aacggccggg gcgaccgcgg tggggacgcg agctgtgcga cgcaacgctc cgatgaagcc
    13321 gagctgagcc acgccgagca ggagtgcgcc ggcgatcggc aagatctgcg ccaccgcacc
    13381 tcccggacgc ggcgcatgac cgtggcgacc gcgtctgggg cgagcgtacc ccgcgggttt
    13441 cgcggtcagc ggacctcggc cgggtgtcgc cctaatcctg tgaaagcgac agcgcctcgt
    13501 agtgcggtat caattcgcgc caacgctgtt gcgccggaac aggttcgcgg ccggcaacat
    13561 gcgcagccag gtcttcggcg tgcatctgca cacccgcgcc gtatgccgcc agctgcgcca
    13621 cggggatgcc gctctcgacg atgacgaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnc cacccggagg tgaagaaggc ggcgcggaac tcgccaccga ggcgcagctc
    13801 gccgtcgacg gtgccgatcc agcgggccag ccggtcgggt tcggtcaggg ccgaccaaag
    13861 atcgtccata ccggtgtcgt aggtgtcctg gacgcgcacc gacccgctgt cgccatcggg
    13921 gtgcagggtc cccaggacgc ggtcgttctt ggcttgggtc atggcgtgct ccttcggtcg
    13981 cgtgtgccgc gggcgatttc ggtatgcagt gcgtcgagcc gctgctccca cctcgcccga
    14041 taggtggtga gccagtcctc cacctcggtg agggggtcgg gttgcaggcg gtagatccgc
    14101 cgctgcgcat cggcctcgac ggtgacgagc ccggcctcgc gcagcacgcg caggtgcctg
    14161 gagacccccg gccgggcgat ggggaggagg tcggccagtt cggtgaccgt ccgcgggcgc
    14221 tcggcgagcg cctcgagcat ggtgcgccgg ctctcgtccg cgagtgctcg cagtgccgct
    14281 tccatcctgt taatgtaccg aagtggttac gtacccgtcg aggtacatac tggcccgtgg
    14341 ccattccgag aggaaagaac tgctatctcg acgggtcgag tcgtcatctc gacggtgggg
    14401 ggtggctcag tcggagagtt cgggatccgg gtcgggcacc atggcggtga tgttcaggcg
    14461 ggcgtcacgg gcggaggtgc gctcggcgtt ggcctcgcga tcgtcggaca tctgctggct
    14521 ctcgagttcg gccatgacgc gctgggcata caactgcacc tccatggcgg tccgcgcctc
    14581 gtcccagccc aacaccgggg ccacaagctc ggccactgcc tgagccgaat ccactccgcg
    14641 agagagggtt tcgatggaag cccgggtgcg gcgggcgagc aggtcatcaa ggtgcagggc
    14701 accttcgtgt gtgacgccat agaggatctc gaccttgagg tattcctcgg cgcccggcac
    14761 cgcctcatac agcgtcgggt cgccctcggc gagttcgaag acctcgtcga cgagggaccc
    14821 gtagcggccg agaaggtgcg tgaggcgcca taccggcagg tcgaccttgc gggagaggcg
    14881 gtcgatctgg ttcatcagcg cgggatagcc gtcggcgccg agcagcggga tgtgctcggt
    14941 gacgctgtcg gccacgcccg ggccgatgtc ctcgcgggcg gcgtcgatgg cgtcggcggc
    15001 catgacgcgg tatgtcgtgt acttgccgcc ggcgatcgag atcaggccgg gctggggccg
    15061 ggcgacagcg tgttcgcggg agagctggga ggtctgctcg tcctcgccgg agagcagggg
    15121 ccgcagcccg gcatacacgc cctggatgtc ggcgtgggtc accgggtggg tgagcaccga
    15181 attgacttgc tcgagaaggt aatcgatgtc tgcctgactc gccgctgggt gggccaggtc
    15241 gaaggtccag tcggtgtccg tggtgccgat gatccagtgc gtgccccagg gcaggacgaa
    15301 gagcaccgac gtctcggtgc gcaggatcag gcccacttcg gagttgatcc ggtcgcgggc
    15361 gatgacgatg tgcacgcctt tggaggcccg gacgttgaag cggccgcggc cgccggcgag
    15421 ggtctggatc gcgtcggtcc acacgccggt gcagttgacc acgacggacg ccttgacggt
    15481 ggtgcgggcg ccggtctcga tgtcctcgac cacggccccg gtgatccgct cccccgccct
    15541 ggtcagctcg accacctggg cggagttgag caccgtggcg ccgtatgccg cggccgtgcg
    15601 ggcgacggtc agggtgtgcc gcgcgtcgtc catctgcccg tcgtagtaga gcagcgcgcc
    15661 ggtgagcgcg tcctttcgca acgcgggcac caggcgcaac gcgccgttgc ggctgagctg
    15721 cttgtgcccc gggaccgagc gggcgccgcc catggtgtcg tagagcgtca gcccggcggt
    15781 gacatagggc cgctcccagc cgcggtggct gagcgggtag aggatcggcg cggtgatcgg
    15841 gttcatgctg atgaccgcgc c
//
LOCUS       DAOPJG010000065        15414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4028_length_15414_cov_7.908002, whole genome shotgun sequence.
ACCESSION   DAOPJG010000065 DAOPJG010000000
VERSION     DAOPJG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 15414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15414
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_15414_cov_7.908002"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1111
                     /gene="coaA"
                     /locus_tag="PKU75_07830"
     CDS             155..1111
                     /gene="coaA"
                     /locus_tag="PKU75_07830"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HPF80823.1"
                     /translation="MCAVPLTRSDAGLPSPYAEFDRQEWARLREEHPMSLTQDDLTRL
                     RGLGDPLDLQEVEEVYLPLSRLLHFYVEATYGLRLATSEFLGERPSRVPFIIGVAGSV
                     AVGKSTTARILKELMSRWPHTPRVELVTTDGFLLPNAELERRGLMQRKGFPESYDRRA
                     LLHFVADVKSGMPLVEAPTYDHLTYDVLPDMHAIPKPDVLILEGINVLQPAPDRTDAL
                     AVSDFFDFSVYVDADPAEIERWYVERFLRLRRTAFADPDSYFRRYAALSDDEARETAL
                     GIWKTINEPNLVENVLPTRGRATLVLTKGPHHKVERIRLRKV"
     gene            1216..1767
                     /locus_tag="PKU75_07835"
     CDS             1216..1767
                     /locus_tag="PKU75_07835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80824.1"
                     /translation="MERLRTYSRDLEDIPPAPRSVGRTLVAADAAAVEDFHAREPRAF
                     SSRKREVLLGRLGTAEQVWLIKDGDGSWVGWAHTCRGSGPNSRIHHTLRLGADEVYFF
                     DDATIPARRHQGVHAFGIIARLEAAKAAGARRAVTTITDSNQASIASYTKLGFAPRSI
                     LLHFPDLGRTVELRVPRPRRRGR"
     gene            1768..2976
                     /locus_tag="PKU75_07840"
     CDS             1768..2976
                     /locus_tag="PKU75_07840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80825.1"
                     /translation="MESEILRTTAQLDAVREVWQELDRRDPASEFYTTWAASRAWWEA
                     FGRETGVELRVIVATHNGATVGILPLALETRGGKNPRRVLRFAGHGDWMGPVAAPSLP
                     PATVYRSLITAALDLPGWDLLDLDNIRRGSPFAAHCLADPALNPHFAHRVEHPVIGLR
                     GLTTPAQVPKKARKYRARAVRELGLRFTKHPGDTDGILDRLARLHRLEKEHLVAEHGR
                     SERHSWSESPARLGHYQALFAAPETGAMTYCYETRDGRLLAARSTFRHRYTLLSWTSA
                     YHPDAAAYRLGKALQLDILDDLIATGEADFFDFGAGRYPWKFEWTDAFDTSYRLRIER
                     PHGDPAPPSPAPATPIPQPEPLAEDAGGPATANAPARLPGWARSLGAGRLYWSTRRAL
                     KRGLRKRPAK"
     gene            complement(3089..>3422)
                     /locus_tag="PKU75_07845"
     CDS             complement(3089..>3422)
                     /locus_tag="PKU75_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80826.1"
                     /translation="AAAALAAARKDPQSVARSLLSEYGFGADQFGCLVQLWTGESDWN
                     YRAANPSSGAYGIPQSLPGSKMASAGADWQTNPATQIRWGLGYIKASYGTPCGALSAW
                     QSRYPHWY"
     assembly_gap    3423..3522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3523..3905)
                     /locus_tag="PKU75_07850"
     CDS             complement(<3523..3905)
                     /locus_tag="PKU75_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80827.1"
                     /translation="MRQFALPTPARRTAGALTAAALTVSGVAAITAAASSASAALPAT
                     ATLAAVSHPASAVNELTMQRAEQAISRSHQRAAVQAVTESRLQRQAQAAAQSADAIDV
                     HKKQQAKAAKAAKAAAAKKAAAKRAA"
     gene            complement(4220..5620)
                     /locus_tag="PKU75_07855"
     CDS             complement(4220..5620)
                     /locus_tag="PKU75_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPF80828.1"
                     /translation="MAVTTGPAPARRPRTTRPSTSEPAATAPAAGVTPSGEPRRTYVV
                     DTSVLLSDPKAMLRFKEHEVVLPVVVVSELEGKRHHPELGYFARTALRTLDDLRIEHG
                     RLDSPVPVGDEGGSLRVELNHTDSSSLPDGFRLGDNDTRILSVAKNLAGEGYLVTVVS
                     KDLPMRVKASAVGLDAQEYRAEMAVESGWTGFAELEVTAEELDELYDNGRLEHASAAE
                     MPCHTGLKLLSPRGSGLGRVGPDKQIRLVRGDRDAFGLHGRSAEQRIAVDLLLDPDVG
                     IVSLGGRAGTGKSALALCAGLEAVMERRAHRKVVVFRPLYAVGGQELGYLPGSENEKM
                     GPWAQAVFDTLSAVVSTEVVEEVLDRGMLEVLPLTHIRGRSLHDAFVIVDEAQSLERN
                     VLLTVLSRIGQNSKVVLTHDVAQRDNLRVGRHDGVAAVIETLKGHPLFAHMTLTRSER
                     SPIAALVTDLLEGVEH"
     gene            complement(5900..6661)
                     /locus_tag="PKU75_07860"
     CDS             complement(5900..6661)
                     /locus_tag="PKU75_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPF80829.1"
                     /translation="MSVGDLIYAAYERRVARGLDRRALPRHIGVMLDGNRRWAAERGA
                     DTKTGHQAGADNIANFLTWCEDIGVELVTLWLLSTDNLSRPERELSPLLEIIEKAVGD
                     LAATSRWRIHAVGALHLLPAPTREALEAAEEATEHVGGMTVNVAVGYGGRQEIADAVR
                     ALLTERAAAGEDITEVAREVTVEQIAEHLYTAGQPDPDLVIRTSGEQRLSGFLLWQSA
                     HSEFYFCEAYWPDFRRVDFLRALRAYADRHRRFGG"
     gene            complement(6672..6986)
                     /locus_tag="PKU75_07865"
     CDS             complement(6672..6986)
                     /locus_tag="PKU75_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80830.1"
                     /translation="MNILIALAAANDPNASEASSGFAGFAVFFLMCLAVWLIFRNMTG
                     RLRRMNRAHDAEEAAAAEQAAAGQPVSERPDPGQPGADRPRAGQPLADERDPAAGDPE
                     NH"
     gene            complement(6983..7939)
                     /gene="mca"
                     /locus_tag="PKU75_07870"
     CDS             complement(6983..7939)
                     /gene="mca"
                     /locus_tag="PKU75_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HPF80831.1"
                     /translation="MSSTERASMTGLRLMCVHAHPDDESSKGAATMAKYVDAGAEVLV
                     VSCTGGERGDVLNPNLKDDPRILRDLAQVRRDEMAAARDILGVQHHWLGFVDSGLPEG
                     DPLPPLPPGCFALEPLEAPVEALVREIRRFRPHVITTYDENGGYPHPDHIRTHEVTIP
                     AFDAAGDPAAYPHAGAPWQPLKLYYNGGFSKERIQTFHEALVAEGLESPYADWLDKWD
                     RPSRTVTTRVQCADWFERRDAALIAHATQIDPDGWFFKVPREMQQRVWPFDEYEAAAS
                     YVPVAEGEDDLFAGLGSLAEADALALDPPPLALDRRVARVVP"
     gene            8002..8406
                     /locus_tag="PKU75_07875"
     CDS             8002..8406
                     /locus_tag="PKU75_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HPF80832.1"
                     /translation="MTAIDPEQLDGDDLDIPTRSAGSRRWWVIGSIGVAAMTAVVVWF
                     AWSATAGRVSWQTRSYEVVDASNVTVSFDVHRPAGMAVTCRLKALNQRFATVGSVDVL
                     IPAGEQRSLTQEGNIRTTSLAVTGTVERCKPA"
     gene            8511..8999
                     /gene="greA"
                     /locus_tag="PKU75_07880"
     CDS             8511..8999
                     /gene="greA"
                     /locus_tag="PKU75_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPF80833.1"
                     /translation="MSEQPTATYLTQEAFDRLTAELAELQGPRRQEVIARIEAARAEG
                     DLKENGGYHAAREEQGKLELRIRQLTQLLETAEVGATPPPDDGVVEPGMVVTVELFGD
                     EETFLLGSREIADGSMQVFSEASPLGAAINGLKVGDTTSYEAPNGKQIEVTIKHAVPY
                     TG"
     gene            complement(9066..10286)
                     /gene="ilvA"
                     /locus_tag="PKU75_07885"
     CDS             complement(9066..10286)
                     /gene="ilvA"
                     /locus_tag="PKU75_07885"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HPF80834.1"
                     /translation="MASVPTVTLDDIRAARETIGELIRPTPLEFSRAVSEKVGTDVYL
                     KCENLQRAGSFKIRGAYTRLARLSEEEKERGVVAASAGNHAQGVALAATTLGIKSKVY
                     MPVGAAMPKLNATRAYGAEIEQVGATLDEAIVEARAYAEQTGAVFISPFDHADIVAGQ
                     GTCGLEILEQCPDVGTVVVSLGGGGLLAGIATAVKAQRPEVMVIGVQAEQAAAYPMSL
                     AAGRPIPAEKMATMADGIAVGRPGDVPFEIVRRLVDRVDTVSEASLSRAMLMLLEHSK
                     LVVEPAGAAGVALLNESADRSWNGPIVVVLSGGNIDPLLMLRVIRHGMVAAGRYLQFR
                     AVMPDRPGSLARLIADCAAADANVVEVEHTRHDANLLIDEVDILVQLETKGPQHCQDV
                     LTALRSKGYRLKFT"
     gene            complement(10559..11869)
                     /locus_tag="PKU75_07890"
     CDS             complement(10559..11869)
                     /locus_tag="PKU75_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HPF80835.1"
                     /translation="MTRAACVDVGSTFTKGLLVDLADGRVCGRAASPTTSGTDVLEGL
                     DAVLAAVGASPADEVLVCSSAGGGLRIAVIGYEEAVTALAGHRVALSAGGRVVHVAAG
                     RLDREAVGALRRARPDVLLLVGGTDGGNSDVLVHNATRLANARLAAPVVLAGNRDAAA
                     ECVAVLKGKGRTVIVADNVLPRIGELAPARAREAIREVFLRHVIGGKGLSRGPRFASL
                     VRMATPDAVLLGVELLASARGEDVLALDIGGATTDVYSALIPRGEDAGLRREISPAPR
                     VLRTVEADLGMRWSAPGIVEGAAREHLPLSAGVGEYAQSVHEDIELLPASPADWDREI
                     ELATAAALLAVRRHARPGHPGDAPRALKEAGVLIGSGGVLRHAPDGRGDAVLAAVLAD
                     HGGGWVVPVAARYAVDTAYLLFAAGLLSRDHPAAAAHLARVIGG"
     gene            complement(11866..12249)
                     /locus_tag="PKU75_07895"
     CDS             complement(11866..12249)
                     /locus_tag="PKU75_07895"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HPF80836.1"
                     /translation="MQPYESHIERLIREATERGEFDNLRGAGKPLDLGSPDDPDWWIK
                     QKLRAEGLDGAGAVPAVLSLRKEAAGFPESLREIRTEAGVRTVLEDDNDRCRRDRLMP
                     RDPKTPPLVAPILDVDAMVARWRQL"
     gene            12391..13461
                     /locus_tag="PKU75_07900"
     CDS             12391..13461
                     /locus_tag="PKU75_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPF80837.1"
                     /translation="MGRARRARKIAATAAYGGGGLAATAGALGALGYGLIKVEAKIAR
                     RIVGQPFEGAPEDDAVYGYGVGERTEIIVLGDSSAAGMGAETGAQTVGGIIATGLAAL
                     TGRPVRLTNEAVIGAESSDLERQLANALDRVERPHVAVILIGANDVTHRIDRATAVRH
                     LELVVQRLRGMGVEVVVGTCPDLGTVEPIPIPLKHLLRRWSRDLAAAQTVAVVEAGGR
                     TVSLGDLLGPEFSAAPKELFSEDQFHPSPAGYARVAAVLLPTVCAALGVGTAGDTQDR
                     RPEPDRGETVEPVAVAAGHAVLDPGTEVSGTDLAGVDRGPRGRWAVLLRRRTSEVPPP
                     APAPETAESREADGEDAPNPVE"
     gene            complement(13520..13837)
                     /locus_tag="PKU75_07905"
     CDS             complement(13520..13837)
                     /locus_tag="PKU75_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80838.1"
                     /translation="MPAPLPEAVADELTRLVRRWQALPLDQALSFSAPVRALAQQYAA
                     ARHPGIPLPDLGPAVLPDQLTVVLYDALAAGDVTPAAAHADLVGLRRTLSARPNAGSR
                     PVE"
     gene            complement(13844..14746)
                     /locus_tag="PKU75_07910"
     CDS             complement(13844..14746)
                     /locus_tag="PKU75_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HPF80839.1"
                     /translation="MSAAGKVTAPELSRRTRVMASNPVFDRIEREGRQGYAGFGNQPT
                     TQAYGQPYGQQSYPTPGQLQDMYNRPSAGPNQTGRVTMDDVVMKTLTLFGIVLATGAF
                     GWIVSDQNRALGQMFWMGGMIGTLILGLVIAFKKTISVPLIIGYAALEGLFVGAVSNF
                     FNDFYGEGLVSTAVVATLATFAGMFLAYKSGLIKVTNKFRRIVTLMIFGYAIFAVVNF
                     IFAMVSDTPFGFGGTGGLGIALSLFAVGLASLSLALDFDSIDQAVRTGAPQQYSWLLA
                     HGLIVTLVWLYIEFLRLLARLRSE"
     gene            complement(14841..14915)
                     /locus_tag="PKU75_07915"
     tRNA            complement(14841..14915)
                     /locus_tag="PKU75_07915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14879..14881),aa:Leu,seq:taa)
BASE COUNT         2294 a         5368 c         5463 g         2189 t
ORIGIN      
        1 ggccttctcg tcgacgttcg gaccgacata cccaacgatt ccacacatag ctcgcaagcc
       61 tacggccgcg tccggcccac ccccgggagc cacgcaccgg tcttcgcacg cccgggcggg
      121 gcccgacgcc ccgccccccg cggcatacgc aacaatgtgc gccgtgcccc tgacccgctc
      181 cgacgccggc ttgccgagcc cctacgccga gttcgaccgg caggagtggg cgcgcctgcg
      241 cgaggagcac ccgatgagcc tgacgcagga cgacctgacg cggctgcggg gcctcgggga
      301 tccactggat ctgcaggagg tcgaggaggt ctacctcccg ctgtcccggc tgctccactt
      361 ctatgtcgag gcgacctatg gactgcggct cgcgacgtcg gagttcctcg gcgagcggcc
      421 ctcgcgggtg ccgttcatca tcggcgtcgc cgggtcggtc gcggtcggca agtcgaccac
      481 cgcccgcatc ctcaaagagc tgatgtcgcg ctggccgcac acgccgcgtg tcgaactcgt
      541 cacgaccgac ggattcctgc tgcccaacgc cgagctggag cgtcgaggac tgatgcagcg
      601 caagggtttt cccgaatcct acgaccggcg cgccctgctg cacttcgtcg ccgacgtcaa
      661 gtcgggtatg ccgctggtcg aggcaccgac atacgaccac ctcacctatg acgtgctgcc
      721 cgacatgcac gcgatcccga agccggacgt gttgatcctg gaggggatca acgtgctcca
      781 gccggcaccg gatcgcaccg atgccctggc cgtgagcgac ttcttcgact tctccgtcta
      841 tgtcgacgcc gacccggccg agatcgaacg ctggtatgtc gagcgtttcc tgcggttgcg
      901 ccgcaccgct tttgccgatc ccgacagcta cttccggcgg tatgccgcgt tgtccgacga
      961 cgaggcgcgt gagaccgcgc tggggatctg gaagacgatc aacgaaccga acctcgtcga
     1021 gaacgtcctg cccacgcgcg gccgcgccac cctggtgctg accaagggcc cgcaccacaa
     1081 agtcgagcgg atccgcctgc gcaaggtatg acgctgggcg agcgcgcgtt tggccgcctc
     1141 gctcgcgtgg ggcactatgg gtgcccgaac gccgggagcc ggcgagaggt tggggagcgt
     1201 atgaagcggt tccgggtgga acgtttgcgt acctactctc gtgacctgga ggacatccct
     1261 ccggcgccgc ggtccgtcgg gcgcacgctg gttgctgcgg acgcggccgc tgtggaggat
     1321 tttcacgccc gggagccgcg cgcgttctcg tcccgcaagc gcgaggtcct gctgggacgg
     1381 ctgggtaccg ccgaacaggt gtggctgatc aaggacgggg acgggtcgtg ggtcgggtgg
     1441 gcgcacacgt gccgcggttc tggccctaac tcccggatcc atcacacgct gcggctgggc
     1501 gcggacgagg tgtatttctt cgacgacgcg acgattccgg cccggcggca ccagggggtg
     1561 cacgccttcg gcatcatcgc ccggctcgaa gccgccaagg ccgcaggggc gcggcgagcg
     1621 gtcacgacga tcaccgacag caaccaggcc tcgatcgcgt cgtacaccaa gctcgggttc
     1681 gccccccgca gcatcctgct ccacttcccc gatctcggcc ggaccgtgga actgcgcgtt
     1741 ccgcgaccac gacgcagggg ccgctgagtg gagagcgaga tcctgcgcac gacagcgcag
     1801 ctggacgcgg tccgcgaggt gtggcaggag ctggaccggc gggatcccgc gagcgagttc
     1861 tacacgacat gggcggccag ccgcgcctgg tgggaagcgt tcggtcgcga aaccggtgtc
     1921 gagttgcggg tgatcgtggc cacgcacaat ggcgccacgg tgggcatcct gcccctcgcc
     1981 ctcgagaccc gtggcgggaa gaatccgcgc cgggtccttc gcttcgccgg ccacggagac
     2041 tggatggggc cggtggccgc ccccagcctg ccgccggcca ccgtctaccg gtcactgatc
     2101 acggccgcgc tcgacctgcc cggctgggac ctgctggatc tggacaacat ccgccgcggg
     2161 tcgcccttcg ccgcccactg cctcgccgac cccgccctga acccccactt cgcgcaccgc
     2221 gtcgaacacc ccgtcatcgg tctgcggggg ctgaccaccc cggcacaggt gccgaagaag
     2281 gcccgcaaat accgcgcccg ggccgtacgc gaactcggcc tgcggttcac gaaacacccc
     2341 ggcgacacgg acggcatcct cgaccggctc gctcgactgc accggctgga gaaagagcat
     2401 ctcgtcgccg agcacggccg ctccgaacgt cactcgtggt cggaatcccc cgcgcggctc
     2461 gggcactatc aggcattgtt cgccgccccc gagaccgggg cgatgaccta ctgctacgag
     2521 acccgcgacg gccggctgct cgccgctcgc tccaccttcc ggcaccggta caccctgctg
     2581 agctggacca gcgcctacca cccggacgcc gcggcatacc ggctcggcaa ggccctccaa
     2641 ctggacatcc tcgacgacct catcgccacc ggcgaggcgg acttcttcga cttcggcgcc
     2701 ggacgctacc cgtggaaatt cgaatggacc gacgccttcg acaccagcta ccggctccga
     2761 atcgaacgcc cgcacggcga cccggcacca ccgtccccgg ccccggccac ccccatccca
     2821 caacctgaac ctctcgctga ggatgcgggc gggccggcca ccgcgaacgc gcccgctcgc
     2881 ctgcccgggt gggcccgcag cctcggcgcc ggccggctct attggtcgac gcgacgcgcg
     2941 ctcaaacgcg gtctccgcaa acgcccggcg aagtagtgcg gacccgccgg cgaggcgcgc
     3001 ggcgcgcgcg ccgcgcgaac tcgtgagatt gcccgccgcg cagcgccctg ctgctcagcc
     3061 gacgtggcgg gagtaggacg acgaaggctc agtaccagtg ggggtagcgg gactgccaag
     3121 cggacagggc gccgcagggg gtgccgtacg aggccttgat gtagccgagt ccccagcgga
     3181 tctgggtcgc gggattggtc tgccagtcgg cgccggcgga ggccatcttg gagccgggca
     3241 gggactgcgg tatgccgtag gcgcccgagg aggggttggc ggcccggtag ttccagtcgc
     3301 tctcgccggt ccacagctgg acgaggcagc cgaactggtc cgcgccgaag ccgtactcgc
     3361 tcagcaggga gcgggccacc gactgcgggt ccttgcgggc ggccgcgagc gcggcagcgg
     3421 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcggccct cttggcggcg
     3541 gccttcttcg cggcagcggc cttggccgct ttggccgcct tggcctgctg cttcttgtgg
     3601 acgtcgatgg cgtcggcgct ctgcgccgcg gcctgcgctt gccgctgcag acggctctcg
     3661 gtcacggcct ggacggcggc gcgctggtgt gaccgggaga tggcctgctc ggcgcgctgc
     3721 atcgtcaact cgttgacggc cgaggcgggg tgggagacgg cggccagcgt ggcggtcgcg
     3781 ggcagggccg cactggcgct gctcgcggcg gcggtgatgg cggcaacgcc ggacacggtg
     3841 agggcggcgg cggtcagggc gcccgcggtg cggcgggcag gggtgggcag cgcgaattgg
     3901 cgcatgggac ggtgctcctg ggtgggggga ggttcggatg gcccacggca aggcaccgcg
     3961 agagcagcgc gccacgaggg aaaagggccg ggccgcagca ttgcggccgc cgacgtcggc
     4021 aggtgttcac ggcgtcgtta tcaaaaagat actttacccg gtggctaagc ggtcaccaaa
     4081 tccgataggg gaccgggtgc tccaggtctg cggcacatgg cggccgggga gacacgaccc
     4141 gagtgcgggc cataggagta tggcccgcac tcgaggtttc gatatcgggc gggaaacctg
     4201 caggtcccgc cccgctggat cagtgctcga cgccctccag caggtcggtg acgagggcgg
     4261 cgatggggct gcgctcggag cgggtcaagg tcatgtgcgc gaagagcggg tggcccttga
     4321 gggtctcgat gacggcggcg acaccgtcgt ggcggccgac ccgcaggttg tcgcgctgcg
     4381 cgacgtcgtg ggtgagcacg accttggagt tctgcccgat ccgcgagagc accgtcagca
     4441 gcacattgcg ctccagcgac tgtgcctcgt cgacgatgac gaaggcgtcg tgcagggacc
     4501 ggccgcggat gtgcgtcagc ggcagcacct cgagcatccc gcggtcgagc acctcctcga
     4561 ccacctcggt cgagaccacc gcggacaggg tgtcgaagac ggcctgtgcc caggggccca
     4621 tcttctcgtt ctcgctgccc gggagatagc cgagttcctg cccgcccacg gcatacagcg
     4681 gacggaacac gacgaccttg cggtgcgcgc gtcgctccat caccgcctcc aggcccgcgc
     4741 acaaggccag cgcggacttg cccgtcccgg cgcgtccgcc gagcgagacg atgcccacgt
     4801 cggggtccag cagcaggtcg acggcgatgc gctgctcggc gctgcggccg tgcagcccga
     4861 aggcgtcccg gtcgccgcgc acgagccgga tctgcttgtc cggcccaacg cgtccgaggc
     4921 cggagccgcg cggcgagagc agcttgagcc cggtgtggca cggcatctca gcggccgagg
     4981 cgtgctccag ccgcccgttg tcatacagct cgtcgagttc ctcggccgtg acctcgagct
     5041 ccgcgaaacc ggtccacccg gactcgaccg ccatctccgc gcggtactcc tgcgcatcga
     5101 gcccgacagc ggaggccttg acccgcatcg gcaggtcctt gctgacaacg gtgacgagat
     5161 agccctcgcc ggcgagattc ttggccaccg acaggatgcg ggtgtcgttg tcgcccaacc
     5221 ggaatccgtc gggcaggctc gaggagtcgg tgtggttgag ttcgacgcgc aggctgcccc
     5281 cttcgtcgcc gaccggcacc ggagagtcca ggcgaccgtg ctcgatccgc agatcgtcga
     5341 gggtccgcag cgcggtgcgc gcgaagtagc cgagctccgg gtggtgccgc ttgccctcga
     5401 gttcgctgac gacgacgacc ggcagcacga cctcgtgctc cttgaaccgc agcatcgcct
     5461 tggggtcgga caacaggacc gaggtgtcga cgacataggt ccggcgcggt tcacccgacg
     5521 gggtgactcc ggcggctggc gcggtggccg cgggttcgga tgtagacggg cgcgtggtgc
     5581 gggggcgacg ggcgggagcg ggaccggtgg tgacggccat acggatctcc ttgcggcgcg
     5641 acgccccacg tgcagggcac ggcgccctag gtcggggtgt gggaccggaa ggcgccgtgc
     5701 gaaacgggcc gggtccggcc ctccccccga aacacgctgt gcgacaacac cgtgtgctcc
     5761 ggcaagatcc tggcctcccg aaggggaccc gggcgggtcc gcctgcggcc aaactagaac
     5821 ctgacgagcg cgatgacgcg aacgcgaaag ggtgtgtcgg gttaattcgg gttaattccg
     5881 ggtgaccggc agggacactt cagccgccga accggcggtg gcggtccgca tacgctcgca
     5941 gcgcccgcag gaagtccacc cgccggaagt cgggccagta ggcctcacag aaatagaact
     6001 ccgaatgggc cgactgccac aacaagaacc cggagagccg ctgttcgccg gaggtgcgga
     6061 tgacgaggtc ggggtcgggc tgcccggccg tatagaggtg ttcggcgatc tgctcgaccg
     6121 tcacctccct cgccacctcg gtgatgtcct cacccgccgc cgcccgctcg gtcagcagcg
     6181 cccggacggc gtcggcgatc tcctgccggc cgccgtaccc gacggcgaca ttgacggtca
     6241 taccgccgac gtgctcggtc gcttcctccg ctgcttcgag ggcctcgcgg gtgggtgcgg
     6301 gcaacaggtg cagggcgccg accgcgtgga tgcgccagcg gctggtggcg gcgagatcgc
     6361 cgacggcctt ctcgatgatc tccagcagcg ggctcagctc ccgctcgggg cgggagaggt
     6421 tgtcggtcga gagcagccag agggtgacca gttcgacgcc gatgtcctcg caccaggtga
     6481 ggaagttggc gatattgtcc gcgccggcct ggtgcccggt cttggtgtcg gcgccgcgct
     6541 cggcggccca gcggcggttg ccgtcgagca tgacgccgat gtgccggggc agcgcccggc
     6601 ggtccagccc gcgggccacc cgccgctcgt aggcggcata gatcaggtct ccgaccgaca
     6661 tgcgttggga ctcagtggtt ctcggggtcg ccggcggctg ggtctcgctc gtccgccagc
     6721 ggctgaccgg ctcgcggtcg gtccgctccg ggctgccccg gatccggccg ctccgagacc
     6781 ggctgaccgg cggcggcctg ctcggccgcg gcggcctcct ccgcgtcgtg cgcccggttc
     6841 atccggcgca ggcggccggt catattgcgg aagatcagcc acacggccag gcacatcagg
     6901 aagaagacgg cgaaacccgc gaacccggac gaggcctcgg aggcgttggg gtcgttcgcc
     6961 gcggccagcg cgatgaggat gttcatggca ccaccctggc aacccgccgg tcgagggcga
     7021 gcggcggggg gtcgagggcg agcgcgtcgg cctccgcgag ggacccgagc ccggcgaaga
     7081 ggtcgtcctc gccttcggcg acggggacgt acgaggccgc cgcctcatac tcgtcgaacg
     7141 gccagacgcg ctgctgcatc tcgcggggca ccttgaagaa ccagccgtcc ggatcgatct
     7201 gggtcgcgtg ggcgatcagg gcggcgtcgc ggcgctcgaa ccagtcggcg cactgcaccc
     7261 gggtggtcac cgtccggctg ggccggtccc acttgtcgag ccagtcggca tacgggctct
     7321 ccaggccctc ggcgaccaac gcctcgtgga aggtctggat gcgctccttg ctgaagccgc
     7381 cgttgtagta gagcttcagc ggctgccagg gcgcaccggc gtgcgggtat gccgccgggt
     7441 cgccggcggc gtcgaaggcc gggatcgtca cctcgtgggt gcggatgtgg tcggggtgcg
     7501 gatagccgcc gttctcgtca taggtggtga tcacgtgcgg gcggaaccgg cggatctcgc
     7561 ggacgagggc ctcgacgggc gcctccagcg gttcgagggc gaagcagccc ggcggcagcg
     7621 gcggcagcgg atcgccttcg ggcagaccgg aatcgacgaa gccgagccag tggtgctgca
     7681 ccccgaggat gtcgcgggcc gccgccatct cgtcgcgccg cacctgggcc aggtcgcgca
     7741 ggatccgcgg atcgtccttg aggttggggt tgaggacgtc cccgcgctcg ccgccggtgc
     7801 aggagacgac gaggacttcg gcgccggcgt cgacatactt cgccatggtc gccgccccct
     7861 tgctcgactc gtcgtcgggg tgcgcgtgca cgcacatcag gcgcaggccc gtcatgctgg
     7921 ccctttcggt ggatgacatg atcgggggac attcttccac cgctgatccc gcaacccacg
     7981 caatcgaccc gaggcctgcc catgaccgcc atcgatcccg agcaactcga cggggacgac
     8041 ctggacatcc cgacgcggag cgccggcagt cgccggtggt gggtgatcgg cagcatcggc
     8101 gtggcggcga tgacggcggt ggtcgtgtgg ttcgcctggt cggcgacggc cgggcgggtc
     8161 agctggcaga cccggtcgta tgaggtggtg gacgcctcga acgtgaccgt gtccttcgat
     8221 gtccaccggc cggcaggcat ggcggtcacg tgtcgcctga aggcgctcaa ccagcggttc
     8281 gcgacggtcg gctcggtcga tgttctcatc cccgcgggcg agcagcgcag cctgacccaa
     8341 gaagggaaca tccgcacgac gtcactggcg gtgaccggga cggtcgagcg ctgcaagccg
     8401 gcctgaccgg acgctttgct aagatcggtg gttcacctcg ggtctggagc gcacgtcatc
     8461 gtgcgatcca gaccctttga ccatcacgta atcgtcacca ggagttcacc gtgagcgagc
     8521 agccgactgc cacctacctg acgcaggagg ccttcgaccg tctgaccgct gagctcgccg
     8581 agttgcaggg cccgcgccgt caagaggtca tcgcccgcat cgaggcggcc cgcgccgagg
     8641 gggatctgaa ggagaacggc ggctatcacg ccgcccgcga ggagcagggc aagctcgagt
     8701 tgcgcatccg ccagctcacc cagctgctcg aaaccgccga ggtcggcgcc accccgccgc
     8761 cggacgacgg tgtcgtcgag cccggcatgg tggtgaccgt cgaactcttc ggcgacgagg
     8821 agaccttcct gctcgggtcg cgcgagatcg ccgacggctc gatgcaggtc ttctccgagg
     8881 cctcccccct cggggccgcc atcaacggcc tcaaggtcgg cgacaccacc tcttatgagg
     8941 cgccgaatgg caagcagatc gaggtcacga tcaagcacgc cgtcccctac accggctgac
     9001 ccgcccgggt cgagcagccc aggaacctct gctagcagag gttcctaggt ttggcggccg
     9061 agccgtcagg tgaacttgag gcggtagccc ttgctgcgca gggccgtcag gacgtcctgg
     9121 cagtgctggg ggcccttggt ctcgagctgg acgaggatgt cgacctcgtc gatgagcagg
     9181 ttggcgtcgt ggcgggtgtg ctcgacctcg acgacattgg catcggcggc cgcgcagtcg
     9241 gcgatcagcc gggccaggga ccccggccgg tcgggcatga ctgcgcggaa ctgcaggtag
     9301 cggccagcgg cgaccatgcc gtggcggatg acgcgcagca tgagcagcgg gtcgatattg
     9361 ccgccggaga ggacgacgac gatcgggccg ttccaggacc ggtctgccga ctcgttcaac
     9421 agcgccaccc ccgccgcgcc ggccggctcg acgacgagct tgctgtgctc gagcagcatg
     9481 agcatcgccc gggacagcga cgcctcggag acggtgtcga cgcggtcgac gagtcggcgg
     9541 acgatctcga aggggacatc gccagggcgg ccgacggcga tgccgtcggc catcgtcgcc
     9601 atcttctcgg ccgggatcgg gcggcccgcg gccagcgaca tcgggtaggc cgcggcctgc
     9661 tcggcctgga caccgatcac catcacctcg gggcgctgcg ccttgacggc ggtggcgatg
     9721 ccggcgagca gtccgccgcc gccgagggag acgacgacgg tgccgacatc cgggcactgc
     9781 tcgaggatct ccaggccaca cgtgccttgt cccgcaacga tatcggcgtg atcgaaaggt
     9841 gagatgaaga cggcgcctgt ctgctcggcg tatgcccggg cctcgacgat ggcttcgtcc
     9901 agcgtggcgc cgacctgctc gatctccgcg ccgtaggcgc gggtcgcgtt cagtttcggc
     9961 atggccgccc ccacgggcat atagaccttg gacttgatgc cgagagtcgt cgcggcgagc
    10021 gcgacgccct gggcgtggtt gccggcgctg gcggcgacga cgccccgctc cttctcctct
    10081 tcgctcaggc gcgccaggcg ggtgtaggcg ccgcggatct tgaacgagcc ggcccgctgc
    10141 aggttctcgc acttgaggta gacatcggtg cccaccttct cgctgacggc gcgcgagaac
    10201 tccagtgggg tcggtcggat cagctccccg atcgtctcgc gcgccgcgcg gatgtcgtcg
    10261 agggtcacgg tgggcacgct cgccatagcg ggcaacacta gtgcccgccc gcggccggtc
    10321 aggccttctc ggtgcgattg cggaaggccg ggatggccag cggtgcgacg acgagcgaga
    10381 tgccgagcga ccacacgatg ctggcgatga ccggatgccg caacggccag gcggcatcgg
    10441 tgcccagcgg cggcgcgttg cccccacaac tcccgcatgg cctgcacgag cgccgagatc
    10501 gggttccatt cggccgggct tttcagcgtc gcgggctttt cgcggaccgg ctgacccctc
    10561 agccgccgat cacgcgcgcg aggtgggcgg ccgccgcggg gtgatcgcgg gagagcaggc
    10621 ccgcggcgaa gagcaggtat gcggtgtcca cggcataccg ggcagccacc gggaccaccc
    10681 acccgccgcc gtggtcggcg agcacggcag cgagcaccgc gtcaccgcgc ccgtcagggg
    10741 cgtggcgcag cactcccccg gacccgatca gcactccagc ctccttcagc gcccggggcg
    10801 cgtcgcccgg gtggccgggc cgggcgtggc ggcgcacggc gagcagcgct gcggcagtgg
    10861 cgagttcgat ctcgcggtcc cagtcggcgg ggctggccgg gagaagttcg atgtcctcgt
    10921 gcaccgactg cgcatactcc ccgacacccg cgctcagcgg caggtgctcg cgcgcggcgc
    10981 cttcgacgat tccgggggcg ctccaccgca tacccagatc cgcctccacg gtgcgcagca
    11041 cgcgcggcgc cggcgagatc tcccgccgca gcccggcgtc ctcgcctcgg gggatgagcg
    11101 ccgaatagac gtccgtcgtc gcgccgccga tgtcgagcgc cagcacatcc tccccacggg
    11161 cgctcgcgag cagttccacg ccgagcagca ccgcgtcggg ggtcgccatc cgcaccagcg
    11221 aggcgaaccg cggcccgcgg gagagccctt tgccgccgat cacatgccgc aggaagacct
    11281 cccggatcgc ctcccgggcg cgtgccggtg ccagctcccc gatccgcggc agcacattgt
    11341 cggcgacgat gaccgtgcgc cccttccctt tcaacaccgc gacgcactcg gccgcggcat
    11401 cccggttgcc ggcgagcacc accggcgccg ccaaccgggc gttcgcgagg cgagtcgcgt
    11461 tgtgcaccaa gacatccgag ttcccgccgt cggtcccacc gacgagcagg agcacgtccg
    11521 gccgcgcccg gcgcagcgcc ccaacggcct cccggtccag ccgcccggcg gccacgtgca
    11581 ccactcgccc accggccgac agcgcgaccc ggtgaccggc gagggcggtg accgcctcct
    11641 catacccgat aaccgcgatc cgcagcccac caccggcgct ggagcacacc agcacctcat
    11701 ccgccggtga cgcgccgacc gcggcgagca ccgcgtccaa cccctcgagg acatcggtgc
    11761 cggacgtggt cggggaggca gcgcgaccgc atacccgccc gtcggcgaga tccacgagca
    11821 gccccttggt gaaggtcgac cccacgtcga cgcacgccgc ccgggtcaca gctgacgcca
    11881 gcgtgcgacc atcgcgtcca cgtcgaggat cggcgcgacc agcggcgggg tcttggggtc
    11941 gcggggcatc agccggtcac ggcggcagcg gtcgttgtcg tcctccagca ccgtgcgcac
    12001 ccccgcctcg gtgcggatct cgcgcagcga ctcggggaag ccggcggcct ccttgcgcag
    12061 cgacagcacg gcgggcacgg cgccggcgcc gtccagacct tccgcgcgca gcttctgctt
    12121 gatccaccag tcgggatcgt cagggctgcc gaggtcgagc ggcttgccgg cgccgcgcag
    12181 gttgtcgaac tcgccccgct cggtcgcctc ccggatcagg cgctcgatat gcgactcata
    12241 tggctgcaca cggcgacttt atgcggaact attgctgttt ggtcaccgtt acccccgtga
    12301 acctggcgcc agcatgccgg accgggggag gatatttctc cctttgacga ggacgacagc
    12361 caagcgctcc cggggaagga ggttcgcgct atgggacgag cgcgacgcgc ccgcaagatc
    12421 gcggccaccg cggcatacgg cggcggcggc ctcgcggcga ccgccggtgc cctcggcgcg
    12481 ctcggctacg gcctgatcaa ggtcgaggcg aagatcgccc gccggatcgt cgggcagccc
    12541 ttcgaggggg cgccggagga cgacgcggtc tatgggtatg gcgtgggcga gcggaccgag
    12601 atcatcgtgc tcggcgactc ctcggccgcg gggatgggcg ccgagaccgg agcgcagacc
    12661 gtcgggggga tcatcgccac cgggctggcg gcgctgaccg gccggccggt gcgcctgacc
    12721 aacgaggccg tcatcggcgc cgagtccagt gacctcgaac ggcaactcgc caacgcgctc
    12781 gaccgggtcg agcgcccgca cgtcgccgtg atcctcatcg gcgccaacga cgtcacccac
    12841 cgcatcgacc gcgcgacggc ggtgcgtcac ctcgagctgg tcgtgcagcg gctgcggggc
    12901 atgggggtcg aggtggtcgt cggcacctgt cccgacctgg gcaccgtcga gccgatcccg
    12961 atcccgctca agcacctgct gcgccgctgg agccgcgacc tggccgccgc ccagacggtc
    13021 gccgtcgtcg aggccggcgg ccgcacggtg tcgctgggtg acctgctggg tccggagttc
    13081 tccgccgcgc cgaaggaact cttcagcgag gaccagttcc atccctcccc cgccgggtat
    13141 gcccgcgtcg ccgcggtgct attgccgacc gtctgtgcgg cgctgggcgt gggaaccgcc
    13201 ggcgacactc aggaccggcg gcccgagccc gaccggggcg agaccgtgga gcccgtggcc
    13261 gtcgcggccg gacacgccgt gctcgatccg ggcaccgagg tcagcggcac cgatctcgcc
    13321 ggagtcgatc gcggcccccg cggccgctgg gccgtgctgc tacgccgccg caccagcgag
    13381 gtgcccccgc ccgcgccggc tcccgagact gccgagagcc gagaggcaga tggcgaggac
    13441 gccccgaacc ccgtcgagtg agcgcgacac gcacagcgtc ccgcgggatg gtcggcgtgt
    13501 cgcgctcact cgacagggct cactcgacgg gccgggaccc ggcgtttggt ctcgctgaca
    13561 aggtgcggcg taggccgacg aggtcggcgt gcgcggccgc gggggtcaca tccccggcgg
    13621 ccagcgcgtc atacagcacc accgtgagct ggtccggcag gacggccggc cccagatcgg
    13681 gcaggggtat gccggggtgg cgggcggcgg catactgctg cgcgagcgcg cggaccggcg
    13741 ccgagaacga caacgcctgg tccagcggca gcgcttgcca ccgccggacc aggcgtgtca
    13801 attcgtcggc gaccgcctcg ggaagcgggg ccggcatcgt cgcttactcg ctgcgcaggc
    13861 gggcgagcag gcgcaggaac tcgatgtaga gccagaccag ggtgacgatc agtccgtgcg
    13921 cgagcagcca ggagtactgc tgcggggcgc cggtgcggac cgcctggtcg atcgagtcga
    13981 agtcgagcgc cagcgacagg gaggccaggc cgaccgcgaa cagcgacagg gcgatgccga
    14041 gaccgccggt gccgccgaac ccgaacgggg tgtcggagac catcgcgaag atgaagttga
    14101 cgaccgcgaa gatcgcgtag ccgaagatca tcagcgtcac gatgcggcgg aacttgttcg
    14161 tgaccttgat caggcccgac ttgtaggcca ggaacatgcc ggcgaaggtc gccagggtcg
    14221 cgacgacggc ggtggagacc aggccctcac cgtagaagtc gttgaagaag ttggacacgg
    14281 cgccgacgaa gaggccttcg agggcggcat acccgatgat cagcgggacg ctgatggtct
    14341 tcttgaaggc gatgaccagg ccgaggatca gggtgccgat catgccgccc atccagaaca
    14401 tctggcccag ggcccggttc tggtcggaga cgatccagcc gaaggcgccc gtcgcgagga
    14461 cgatcccgaa gagggtgagc gtcttcatga cgacgtcgtc catcgtgacg cggccggtct
    14521 ggttcgggcc ggcggaaggc cggttgtaca tgtcctgcag ctgcccgggt gtcgggtagc
    14581 tctgctggcc atagggctgg ccgtatgcct gcgtggtggg ctggttgccg aagccggcgt
    14641 aaccctgccg gccctcgcgc tcgatccggt cgaaaaccgg gttgctcgcc ataactcttg
    14701 tcctccggga cagctcgggc gcggtcacct tgccggccgc gctcatctga gtcaagcgtg
    14761 acagatgatt cttttgttcc cacccgtcgg gcgcccggtt cgcgataacg atcgggatgc
    14821 ggaatcggga gagcgctggg tgcccccgac gggaatcgaa cccgcaccgt tgacgatttt
    14881 aagtcgtctg cctctgccag ttgggccacg gggacagcgg caactctacg acgcccggag
    14941 ccgggccact gctcgtcaac gcccctctcc gggcccgcgg ccgcccaacg atgaccacgg
    15001 gctggatggg ccggtgtctg gggtcgcacg ggtgggccat cggcaacgag aaaacattgt
    15061 tatcagcgcc cgctcgggag tgcggatgaa caagagcagc acgcagcttc caacccgtgg
    15121 tcggcgcggg gccgctcccg ggcacaccgc gggggccccg cgtgccgcgc gccgaacgca
    15181 aggcccagct gatcgccgtc gccgagccgc gtgcagaagc gcggggctgc tgccgcgaac
    15241 gaactcctca cagccggcgt cgacgctcac accgcatgaa acgacgggca ccctccccgt
    15301 gggtggatgc gtaccgccga cgacataaag cctcccatcc tccccgcgct cccgcgtgcg
    15361 acaccgctgc gccggacttg ttgtcgtcgg aggtccgggg ttgtcgccga ggcg
//
LOCUS       DAOPJG010000066        15203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4104_length_15203_cov_7.530103, whole genome shotgun sequence.
ACCESSION   DAOPJG010000066 DAOPJG010000000
VERSION     DAOPJG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 15203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15203
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4104_length_15203_cov_7.530103"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1286)
                     /locus_tag="PKU75_07920"
     CDS             complement(186..1286)
                     /locus_tag="PKU75_07920"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPF80840.1"
                     /translation="MSAVAGVAAPAAADRAGAPGLAVQVVQRNLDIPWDLAFTDSTHY
                     LLNERPGRLWLVNTNPAVPRQRVAADFSGVVTIASGGLMGMVVDPGFATNRLFYTCQT
                     NRTPKDVRVVRWRLSADGTRAVRSGTPVISGIPLGNPHYGCRLLFLPDGSLLVGTGDA
                     ALASGPQSLTSLAGKILRVLPNGSAPRDNPWASATSPKRFVWNYGHRNVQGLAARPGT
                     TQVFSAEHGPDWDDEINLVLKARNYGWDPKGPDGYNQDVPMTDLEKFPNAVPAKWTSG
                     ASTIAPSGISFVTGSAWGTYSGQLFAALLKDEGVLALALDAAGNVRSTAQVPELNDRF
                     GRLRTVRQAPDGSLYVLTANGSTDQVLRVTPK"
     gene            complement(1538..2551)
                     /locus_tag="PKU75_07925"
     CDS             complement(1538..2551)
                     /locus_tag="PKU75_07925"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HPF80841.1"
                     /translation="MGELVLTANAGDGTISTLRLDREAGRLVPLATATGLPGCGTFAV
                     DSARGLVYAAYKGDEPGVATLALDPATGALSQVRRTSVGGAVTYLSLAHGGSFLLGVS
                     YGAGHGWVWPLAEGGRLEEHCGEFSYRNLHCVVASGADVYAVSLGEDLIAQLSLSADG
                     VLAPKEPLVVDAPRGSGARHLCLDGASAYLITEFTGQAIRYDIGADGTLSMAEAVDTV
                     VPGSGLAVSAYGKEPLKEPLIWGADIWRAGDWLLTSERSSSLITSIPVGEGGALGEPA
                     AYSSTEKQPRGFTVTADGAYVVAVGEQSTHAALSRVEADGSLTVLDRAPVGAGANWVR
                     IVG"
     gene            2626..3654
                     /locus_tag="PKU75_07930"
     CDS             2626..3654
                     /locus_tag="PKU75_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HPF80842.1"
                     /translation="MGRLQRIENKLERAINGAFAKAFRAEVQPVEIASAVRRAMDDGA
                     TSLAKGRTFVPTRYRVVLSETDYDRLTAYEDDLGDELVAAAEEHAESQRYLTKNDFGM
                     SFAKDGALETGVFQIVTEDEPAPAAARRMPPARDGDDPRAPARSGERFRSPGEPEPRE
                     PDRAEPQPGEAGWREADRRGAASAPSYGRSPAPDPSLPYDQSLPYDQSLPYDQSRGDQ
                     SRGDHSRDRSREKDPQHHSTPSRMAPADRPWLEIDGDRYPLIGPTTVLGRDTGASIIL
                     DDANISRRHCEIRVTADGPHLVARIKDLGSTNGTWVNGGRADSVLLKTGDRITIGQTA
                     LIFHAGRR"
     gene            3665..4138
                     /locus_tag="PKU75_07935"
     CDS             3665..4138
                     /locus_tag="PKU75_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPF80843.1"
                     /translation="MSELTLTVLRLSLLVLLWLFVFSLAAVLRGDLFGTRVVNRVTTT
                     GGGSGSLAATVPRDRRSSNGNILVTSGRLTGTRIPLGTGDILIGRNPECLLVLDDDFA
                     SGRHARIFPAQGGWGVEDLGSTNGTFVNDVRIEEPVQFEPGAQLRIGRTTIELQK"
     gene            4142..>5442
                     /locus_tag="PKU75_07940"
     CDS             4142..>5442
                     /locus_tag="PKU75_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPF80844.1"
                     /translation="MPHAFHYAARSDVGLVRSENQDSGYAGPHLLVVADGMGGHAGGD
                     IASSTAVGYLVELDDESHSANETGDELAQAIATANAEIGRIVAQRPQLHGMGTTITAL
                     LRSRHTLAIAHIGDSRAYLLRDGELAQITTDHSFVQTLVDSGKITAEEAGSHPQRSLV
                     TRVLTGSRGDQPDLGAREAYVGDRYLLCSDGLSGFVAADTIADVLRAGAAPEETADRL
                     VELALRAGAPDNVTVIVADIVDMTAGRLPPVTPQIVGAATQSRAGNRALRETPAAKAA
                     ALRPTLGLDEESETVERLALAEESSSSRRLLKWLGALAAAVIVIAGGGAALYAWSQRQ
                     YFVGTQDGEVAIYQGISSDLGPIDLSTPLTKTGIKVSDLPDFWRTEVESSITADSRDD
                     AEKRVATIRAQAQACIAARAKGQTCGADTNPGASASSSSSSG"
     assembly_gap    5443..5542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5614..6984
                     /locus_tag="PKU75_07945"
     CDS             5614..6984
                     /locus_tag="PKU75_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPF80845.1"
                     /translation="MSLVTSLRPRRGRNVELLLLLFAVAIITLAYATVEASAHGAWPA
                     NLATQVGGVLAIAVVFHLVLRWTASYADPLLLPIATLLNGLGLVMIHRLDIAKGNELG
                     EGMALRQLTWSALGVAIAIGVLLWLRDHRTLRRYTFLAGTAGVVLLLLPLFPFIGREI
                     NGARIWIGLGPFSFQPGEIAKIVLAVFFAGYLVRTRDVLSIAGPRVLGMQFPRGRDLG
                     PILLMWAVSLAVLFWQKDLGSALLFFGLFVAMLYVATERVGWIAIGLLLAAGGAVIAF
                     RLFGHVQTRVLLWLHTFSPEAVAQSDQLAKGLMGMASGGLLGTGLGQGRPYITYFAES
                     DFIIASFGEELGLVGLMAILILYVLLAERGFRIAVGVRDGFGKLLAAGLAFSLTLQCF
                     VVVGGVTRVIPLTGLTMPFLSSGGSSLLANWTLVALLLRISDQARQPVLDTADSGPIP
                     AAVGGR"
     gene            6981..8438
                     /locus_tag="PKU75_07950"
     CDS             6981..8438
                     /locus_tag="PKU75_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPF80846.1"
                     /translation="MNSPIRRLSAIAAALFFCLLVSSSWIQFAAAKKINDLPDNKRTL
                     LASYNRERGSILVGGEAIARSVATDGELKWDRRYPMAQLYSHVTGYYSYVYGAGGGIE
                     GAEDELLSGRSDKLFYRRVSDLLTGKDPVGASLELTINPAAQTAADRGLGTQRGAVVA
                     LDPKTGAILAMVSHPAYDPAELSSHDIGAVEKAWPRLLKDEGDPLINRSIAGDLYPPG
                     STFKLVTAAAALESGTYTQNSTVPGPATLQLPGVAAPLANHDRAPCGPNDKTTLIHAL
                     EISCNTAFASLGMQLGAEALREQAGKFGFGQTLTVPMRATASSVPANMNEPNTAQAAI
                     GQYDVRTTPLQMAMVAAAIGNGGEVMKPYLVRRVLGSQLQTIDEATPERLSEAVSTST
                     ARQLTTMMKDVVTSGTGTAAQIAGVDVAGKTGTAQHAKGAAPHAWFVSFAPAADPKVA
                     VAVVVEDGGNAGSEASGGAVAGPIAKSVMEAVLNS"
     gene            8557..10428
                     /gene="pknB"
                     /locus_tag="PKU75_07955"
     CDS             8557..10428
                     /gene="pknB"
                     /locus_tag="PKU75_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HPF80847.1"
                     /translation="MTIHVPKLLGGRYEVGELIARGGMAEVHHGYDTRLGRTVAIKIL
                     RSDHVRDAHFLSRFRREAQSVAGLNHRNIVAVYDSGEDQLTETGGARVPVPWIIMEYV
                     DGYTLRELLNEHGDLPPQEAARITESVLDALAYSHEMGMVHRDIKPANVMVAGDGTVK
                     VMDFGIARAVADTQATMTHTSSVMGTAQYISPEQAEGLTVDSRSDLYSTGCLLFELLT
                     GRTPFVGEPVSLTYQHVSKPPPLASSLNPAVSEDLDAVIALALTKDREQRYQEAEDFR
                     DDLRSVRLGRPVSAAAYAALRADQPTAVVAPAQHIPPTPVDVLAPEQPYDEPRRRGSG
                     VLIASILLLAVGVLGFAAKTYLDSRPEYVTVPSVVGKTLTKASSDLQAAGFEVSSSYQ
                     PSSEYDKDKVMDQDPKGGTEQLAGKQINLTVSGGPAATRVPPLAGLTLDEATAALEDA
                     GLELGDVRSLDSPDAKKDEVVSSTPEEYADAAEGDEVDLTVGTGKVALPDLVGKTQDE
                     ARAILAELKLPVLTVLEESSETEDTVLRTNPKKGKVNVGTTVTLTLAKPRTIVQTTTI
                     TTTQSSTPPPTEPTTSTDTATSTSTTPPPTDTGTGGGTNTGNTIPTISQTSAPPLRN"
     gene            10444..11097
                     /locus_tag="PKU75_07960"
     CDS             10444..11097
                     /locus_tag="PKU75_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPF80848.1"
                     /translation="MAKILVVDNYDSFVFTIVGYLQQLGAETTVVRNDAITPDEGTGF
                     DGVLISPGPGTPEEAGVSMAMIAACAERSQPMLGVCLGHQALGVVMGATVGRAPELLH
                     GKTSQVHHDGTGVLDGLADPFTATRYHSLTIDPPTLPDSLVANGHTDSGIIMAVRHRD
                     LPLHGVQFHPESVLTHGGHRLLANWLAVCGDPAAVAAAEGLSPLVRDAAGIARVVAG"
     gene            complement(11134..11952)
                     /locus_tag="PKU75_07965"
     CDS             complement(11134..11952)
                     /locus_tag="PKU75_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPF80849.1"
                     /translation="MTEQEPQPDRRAARRSAARVAASRWRRWLVLTRRPTMWSLLVPI
                     VAVLAGILFSTSGQASRGTDLRSDAPGLADVIRERQRLMADRVGEVAAVRADVDRLTA
                     AAAPGSERIAKLSGAADALAAGAGTAAVEGDTLTVALDDSPLTSDQLPEWATVDDIVV
                     HQQDVQAVVNALWRGGAEAMMLMDQRVISTSAVRCVGNTLILQGRVYSPPFVISVIGD
                     PAALEAALDADPTIDRYREYVSALGLGYAVERTGIKTFPAFAGTLSLTSARVAD"
     gene            12047..12301
                     /locus_tag="PKU75_07970"
     CDS             12047..12301
                     /locus_tag="PKU75_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HPF80850.1"
                     /translation="MPESKGRKKATYTPQEAPRAQKVKTGNPSWYVPVMLGLMILGLL
                     WVVTFYITKQQYPIEALGRWNLGVGFALMLAGFGMTTNWR"
     gene            complement(12548..13387)
                     /locus_tag="PKU75_07975"
     CDS             complement(12548..13387)
                     /locus_tag="PKU75_07975"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPF80851.1"
                     /translation="MTEPYAGPGAPAPVCPRHPDRISYVRCQRCGNPTCPECQRAAAV
                     GVQCVSCVAQSAREHPTTKTVLGARTGDRGGRPIVTLILIAACVGIWLLEQASRSDEI
                     FYRFALSPAIGEEEPWRFVTSAFLHGPPMHILFNMFALWMLGSYLEPLLGKVRFLILY
                     LVSAIGGGVAYILLNPVTATGGVVGASGAVFGLFGALIVLNRALGRSTQGIMLNLAIN
                     AALPLLYPNIAWQAHVGGFLTGLLLAFVFARGRRNAAVAYGGAAAVTGAIVAAGVARY
                     LTG"
     gene            complement(13457..13972)
                     /locus_tag="PKU75_07980"
     CDS             complement(13457..13972)
                     /locus_tag="PKU75_07980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPF80852.1"
                     /translation="MKATLHTNLGDIVVELFDNQAPKTVANFTGLASGEKEYKDDAGR
                     SNPTKFFDGLGFHRIIPGFMIQGGCPLGQGIGGPGYTFDDEIHPELAFDRPYLLAMAN
                     AGKRMGKGTNGSQFFITVEPTPWLQGKHTIFGEVSDDASRQVVDAIAAVPTGPNDKPV
                     EPVVIESVELS"
     gene            14104..15135
                     /locus_tag="PKU75_07985"
     CDS             14104..15135
                     /locus_tag="PKU75_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80853.1"
                     /translation="MLCSKPPSKTEKAKAAVADYVNTAAEKAAEMREEAADRASHRAS
                     YVADQSADARAAAAHRAAELRDRAAAASEKAGERASHLMERAAPAVDVAKERAAAARE
                     AAAHGYDAAAPRVETARNTLVEDVLPKVAGAIATVAAGALSAKDQAAETAHRAPEAFA
                     VLKGEATAKPKKRSWGPFLLLAALAGGAAYVLTRRKTPATDPWAVPASDPYAARSPMA
                     GDTGSSLQDKAASVAAAAKDKAAEVKDTVADKAAEVKDKASDKAAELKDKAGDAADDA
                     KDATSDAFDDAKGQVSEAQDAAGDALDETGDNLGDRIENATDTAADSINDAADRVEDA
                     GDRTKDNWG"
BASE COUNT         2378 a         5551 c         5101 g         2073 t
ORIGIN      
        1 ccccggacgg cgtcaagatc gcgttcgacg ccaaattctg gaacaacccg atctcgcagc
       61 aggtgtgcct gtgcgacacg gccggcacca ccgcgaccag cgtcagcctc ccgacgggcc
      121 agtccgccgc ctgcccggtc ggctagggct ggcgccgccg cggaccaggg cggtgtcgag
      181 accgactact tgggcgtgac ccgcaagacc tggtcggtgc ttccgttggc ggtcaggacg
      241 tacagggatc cgtcgggggc ctgacgcacc gtgcgcaggc ggccgaagcg atcgttgagt
      301 tcgggcacct gggccgtgga gcgcacattt ccggccgcat ccagcgccag cgccagcaca
      361 ccttcgtcct tcaggagcgc ggcgaacagc tgaccggagt aggtgcccca cgcactcccc
      421 gtcacgaagc tgatgcccga cggcgcgatg gtggacgcac cggaggtcca cttggccggg
      481 accgcgttgg ggaacttctc gaggtccgtc atcgggacgt cctggttgta gccgtccggt
      541 cccttcggat cccagccgta gttgcgcgcc ttgagcacca ggttgatctc gtcgtcccag
      601 tcgggtccgt gctcggcact gaagacctgc gtggtccccg gccgcgccgc aagtccctgg
      661 acgttgcggt gcccgtagtt ccagacgaac cgtttcggac tcgtggcgct tgcccacggg
      721 ttgtcacgcg gagccgagcc gttgggcaga acccgaagaa tcttgccggc gaggctggtg
      781 agcgactgcg ggccggaggc cagggcggca tcgccggtgc cgaccaggag gctgccgtcg
      841 gggaggaaga gcaggcgaca cccgtagtgc gggttgccga gcggtatgcc gctgatcacc
      901 ggagtcccgg atcgcaccgc tcgcgtgccg tccgccgaga gccgccaccg caccacccgc
      961 acgtccttcg gagtgcggtt ggtctggcac gtgtagaaga gccggttcgt ggcgaatccc
     1021 gggtcgacca ccatccccat caatcctccg ctggcgatcg tgaccacgcc ggagaagtcc
     1081 gcggccacgc gctggcgcgg aacggccgga ttggtgttga ccagccagag ccggccgggc
     1141 cgctcgttga gcagatagtg cgtggaatcg gtgaaggcga gatcccatgg gatgtccaga
     1201 ttgcgttgca ccacctgcac ggccagcccg ggagcaccgg cgcggtccgc cgcggcgggc
     1261 gcggcgaccc cggcaacggc gctcaacccg cacaccacca gcgcaccgac ggtcgccgcg
     1321 aggggccgcg acgtgcgacg ggcagggagc cggcgcgacg gacgaaatgc catagctctc
     1381 accaagccga tcacgagatg cggagttcta tcccagtgaa ctacgcgacc gggttcgtgt
     1441 catgtcctac tgatgagcgt cacgcaccga gcaggaccac gtgtgagccc aggacccgtc
     1501 actgtccccg cccagcggtc gggcgacgcc cggccggtca gcccacgatg cgcacccagt
     1561 tggccccggc gccgacgggg gcgcggtcga gcaccgtgag cgagccgtcc gcctcgacgc
     1621 gcgacagggc ggcgtgggtg gactgctcgc cgacggccac cacataggcg ccgtcggcgg
     1681 tgaccgtgaa cccgcgcggc tgcttctcgg tgctgctgta tgccgcgggc tcgcccaatg
     1741 ctcccccctc acccaccggg atgctcgtga tgagggagga ggagcgctcg gaagtgagca
     1801 gccagtcgcc ggcccgccag atgtcggccc cccagatgag tggctccttc aagggctcct
     1861 tgccgtaggc gctgaccgcc agccccgagc cgggcaccac cgtgtcgacc gcctcggcca
     1921 tcgacagcgt cccgtcggcg ccgatgtcat agcggatcgc ctgccccgtg aactcggtga
     1981 tcagataggc cgaggccccg tcgagacaca ggtgccgcgc ccccgacccc cggggtgcgt
     2041 cgacgaccag cggctccttc ggcgccagca ccccatcggc cgacaacgag agctgggcga
     2101 tcaggtcctc gccgagggag acggcataca cgtcggcgcc ggaggcgacc acgcagtgca
     2161 ggttgcggta cgagaactcg ccgcagtgct cctcgagccg accgccctcg gcgagtggcc
     2221 agacccagcc gtggccggca ccatacgaca cgccgagcag gaacgacccg ccatgggcga
     2281 gggagagata ggtcaccgcc cctccgacgc tcgtgcggcg cacctgcgac aacgcccccg
     2341 tcgccgggtc cagcgcgagc gtggcgaccc cgggctcgtc gcccttgtag gcggcataca
     2401 ccagcccccg tgcggaatcc acggcgaagg tgccgcaccc cggcaacccg gtcgccgtcg
     2461 ccagcggaac caaccgcccg gcctcgcggt ccaagcgcag cgtgctgatg gtgccgtcgc
     2521 cggcgttggc ggtcaggacc aattcaccca tgccgacact ctaaggacgg aacgggtgat
     2581 cagcaccgcc cgttacgatc agcgttggag tcgaggaggt gactggtggg acgattgcag
     2641 cgcatcgaga acaagctcga gcgcgccatc aatggtgcct tcgccaaggc cttccgggcc
     2701 gaggtgcaac cggtcgagat cgcgagcgcg gtgcggcggg ccatggacga cggggcgacg
     2761 tcgctggcca agggccgcac gttcgtgccg acccgctatc gcgtggtgtt gtcggagacc
     2821 gactacgacc ggttgaccgc ctacgaggac gatctcggcg acgaactggt ggccgccgcc
     2881 gaggagcacg cggagtcgca gcgttacctg accaagaacg acttcggcat gagtttcgcc
     2941 aaggacggcg cgctcgagac cggtgtcttc cagatcgtca ccgaggacga gccggcgccg
     3001 gccgcagcac gtcggatgcc gcccgctcgc gatggcgacg atccgcgcgc ccccgcgcgc
     3061 tccggtgagc ggttccgctc cccgggtgag ccggagccgc gggagccgga ccgggccgag
     3121 ccgcagccgg gcgaggcggg ctggcgggag gcggaccggc ggggggcggc gagcgccccg
     3181 tcatacggtc gatcaccggc acctgacccg tcactgccct acgatcaatc actgccatac
     3241 gaccaatcac tgccgtacga ccagtcccgc ggcgaccaat cacgcggcga ccactcccgc
     3301 gaccggtcgc gcgagaagga cccgcagcac cactcgaccc cgtcgcgcat ggcgccggcc
     3361 gaccggccgt ggctggagat cgacggcgac cgctacccgc tgatcggccc gacgaccgtg
     3421 ctgggtcgcg acaccggcgc gagcatcatc ctcgacgacg ccaacatcag ccggcggcac
     3481 tgcgagatcc gggtgaccgc cgacggcccg cacctcgtgg cccgcatcaa ggatctcggc
     3541 tcgaccaatg gcacctgggt caacggcggc cgggcggatt cggtgctgct caagaccggc
     3601 gaccgcatca ccatcggcca gacagcgctc atcttccacg ccgggcggcg gtgatcggcg
     3661 ccgcatgagc gaactgaccc tgacggtcct gcgcctgagc ctgctcgtgc tgctctggct
     3721 gttcgtcttc tccctggcag cggtgctgcg cggcgacctg ttcggcaccc gcgtcgtcaa
     3781 ccgggtcacg accaccggcg gcggcagcgg ctcgctggcc gcgaccgtcc cccgcgaccg
     3841 gcgctcctcc aatggcaaca tcctcgtcac gtccggccgc ctcaccggca cccgcatccc
     3901 cctgggcacc ggcgacatcc tcatcggccg caaccccgag tgcctgctcg tcctcgacga
     3961 cgacttcgcc tccggccggc acgcccggat cttcccggcg cagggcggct ggggggtgga
     4021 ggacctcggg tccaccaatg gcaccttcgt caacgacgtc cgcatcgagg agcccgtcca
     4081 gttcgaaccc ggcgcccaac tgcgcatcgg ccgcacgacg atcgagctgc agaagtaagc
     4141 cgtgccacac gccttccact acgccgcccg ctccgacgtg gggctggtcc gctcggagaa
     4201 ccaggactcc gggtatgccg gcccccacct cctcgtcgtc gccgacggca tgggtgggca
     4261 cgccggcggc gatatcgcct cctcgaccgc ggtcggctat ctcgtcgagc tcgacgacga
     4321 gtcccactcc gccaacgaga ccggcgacga actcgcccag gcgatcgcga ccgccaacgc
     4381 cgagatcggc cgcatcgtcg cgcagcgtcc ccagctgcac gggatgggca cgacgatcac
     4441 ggcgctgctg cgctcgcggc ataccctcgc catcgcccac atcggcgact cgcgcgccta
     4501 cctgttgcgt gacggcgagc tggcgcagat caccacggac cactcgttcg tccagaccct
     4561 cgtcgactcc ggcaagatca ccgcggagga ggccggcagc cacccgcagc gctccctcgt
     4621 cacccgcgtg ctgaccggca gccgcggcga ccagcccgac ctcggggccc gcgaggcgta
     4681 tgtcggcgac cgatacctgc tgtgctccga cgggctgtcc gggttcgtcg ccgccgacac
     4741 catcgccgac gtgctgcggg ccggtgccgc tcccgaggag accgccgacc ggctggtcga
     4801 actcgccctg cgcgccggcg cccccgacaa tgtgacggtc atcgtcgccg acatcgtcga
     4861 catgaccgcg gggcgcctgc ccccggtcac cccgcagatc gtcggcgccg ccacccagag
     4921 ccgcgccggc aaccgcgcgc tgcgcgaaac ccctgccgcc aaggccgccg ccctgcgccc
     4981 cacgctcggg ctcgacgagg agagcgagac cgtcgagcgg ctcgccctcg ccgaggagtc
     5041 ctcctcctcc cggcgcctcc tgaagtggct gggcgccctc gccgctgccg tcatcgtcat
     5101 cgccggtggg ggcgccgcgc tgtatgcctg gtcgcagcgg cagtacttcg tcggcaccca
     5161 ggacggtgag gtcgcgatct atcagggcat cagctccgac ctcggcccga tcgacctgtc
     5221 caccccgctg accaagaccg gtatcaaggt cagcgacctg cccgacttct ggcggaccga
     5281 ggtggagtcg agcatcaccg ccgacagccg cgacgacgcc gagaagcgcg tcgcgacgat
     5341 ccgcgcccaa gcccaggcct gcatcgccgc ccgcgccaag ggccagacgt gcggcgccga
     5401 caccaatccg ggggcgagtg cctcgtccag cagttccagt ggnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nntcgggcag ttccaccggg tcgggcagct cctcgacctc
     5581 gagccgcccg acgagcacga cgacgagccg cgcatgagcc tcgtcacctc gctgcgcccc
     5641 cgccgcggcc gcaacgtcga gctgctgctc ctgttgttcg ccgtcgccat catcaccctc
     5701 gcctatgcga ccgtcgaggc cagcgcccac ggcgcctggc cggccaatct cgccacccag
     5761 gtcggcgggg tgctggcgat cgccgtcgtc ttccacctcg tgctgcgctg gaccgcgtcg
     5821 tatgccgatc cgctgctgct gcccatcgcc accctcctca acgggctggg tctggtgatg
     5881 atccaccgcc tcgacatcgc caagggcaac gagctcggcg agggtatggc gttgcgccag
     5941 ttgacgtgga gcgcgctcgg cgtcgcgatc gcgatcgggg tgctgctctg gctgcgggat
     6001 caccgcacgc tgcgccgcta caccttcctg gccggcaccg ccggtgtggt gctcctgctg
     6061 ctgccgctct tcccgttcat cggacgcgag atcaacggcg cccggatctg gatcggcctg
     6121 gggcccttca gtttccaacc gggtgagatc gccaagatcg tgctggccgt cttcttcgcc
     6181 ggctacctgg tgcgcacccg cgatgtcctg tccatcgccg gaccgcgggt gctggggatg
     6241 cagttccccc gcggccgcga cctcggcccg atcctgctga tgtgggccgt cagcctcgcc
     6301 gtgctgttct ggcagaagga cctcggcagc gccctgctct ttttcggcct gttcgtcgcg
     6361 atgctctatg tcgcgaccga gcgggtcggg tggatcgcga tcggcctgct gctcgccgcc
     6421 ggcggcgctg tcatcgcctt ccgcctcttc gggcacgtcc agacccgggt gctgctgtgg
     6481 ctgcacacgt tcagccccga ggcggtcgcg cagtccgacc agctcgccaa gggcctgatg
     6541 gggatggcca gcggtggcct gctcggcacc gggctggggc agggccggcc ctacatcacc
     6601 tacttcgccg agtccgactt catcatcgcc agcttcggtg aggaactcgg gctggtgggc
     6661 ctgatggcga tcctgatcct ctatgtcctg ctcgccgagc gcgggttccg gatcgccgtc
     6721 ggcgtccgcg acgggttcgg caagctgctc gccgccggcc tcgcgttctc gctgaccctg
     6781 caatgcttcg tcgtcgtcgg cggcgtcacc cgggtcatcc ccctgaccgg cctgacgatg
     6841 ccgttcctgt cctcgggcgg ctcgagcctg ctggcgaact ggacgctcgt cgcgctgctg
     6901 ctgcggatca gcgaccaggc ccgccagccg gtcctcgaca ccgccgacag cggcccgatc
     6961 ccggcggcgg tgggcggccg atgaactccc ccatccgccg gctgtcggcc atcgcggccg
     7021 ccttgttctt ctgcctgctg gtctccagtt cgtggatcca gttcgcggcc gcgaagaaga
     7081 tcaacgacct gcccgacaac aagcgcaccc tgctcgcgtc ctacaaccgc gagcgcggct
     7141 ccatcctcgt cggcggcgag gccatcgccc gctcggtcgc gaccgacggg gagttgaagt
     7201 gggaccggcg ctacccgatg gcacagctgt attcgcatgt caccggctac tactcctatg
     7261 tctacggcgc cggcggcggc atcgagggcg ccgaggacga gttgctctcc ggccgcagcg
     7321 acaagctctt ctaccgccgc gtctccgacc tgctcaccgg caaggacccc gtcggcgcct
     7381 ccctcgagct gaccatcaac ccggccgcgc agaccgcggc cgaccgcggc ctgggcaccc
     7441 agcgcggcgc cgtcgtcgcc ctcgacccga agaccggggc catcctcgcg atggtcagcc
     7501 acccggcata cgacccggcg gagctgagct cgcacgacat cggcgcggtc gaaaaggcat
     7561 ggccgcgcct gctcaaggac gagggcgacc cgctgatcaa ccggtccatc gccggcgacc
     7621 tctacccgcc cggctcgacg ttcaagctcg tcacggccgc cgcggcgctc gagtccggca
     7681 cgtacacgca gaactccacc gtgcccggcc cggccacgct ccagctgccc ggcgtcgcgg
     7741 cccccctcgc caaccacgac cgcgccccgt gcgggcccaa tgacaagacg acgctgatcc
     7801 acgcgctgga gatctcgtgc aacaccgcct tcgcgagcct cgggatgcag ctcggcgcgg
     7861 aggcgctgcg ggagcaggcg ggcaagttcg ggttcggtca gacattgacc gtgccgatgc
     7921 gggcgacggc cagctcggtc ccggcgaaca tgaacgaacc caacaccgcg caggccgcga
     7981 tcggccagta tgacgtgcgc accaccccgc tgcagatggc catggtggcc gccgccatcg
     8041 gcaacggcgg ggaggtcatg aagccctatc tggtgcgccg cgtcctcggc tcccagctgc
     8101 agacgatcga cgaggccacg cccgagcggc tatccgaggc ggtctcaaca agcacagcgc
     8161 ggcaattgac gaccatgatg aaggacgtgg tcacctcggg caccggcacg gcagcgcaga
     8221 tcgcgggcgt tgacgtggcg ggcaagaccg gcaccgcgca gcacgccaag ggcgccgccc
     8281 cgcacgcctg gttcgtctcc ttcgctcccg ccgccgaccc gaaggtcgcc gtcgccgtcg
     8341 tcgtcgagga cggcggcaac gccggcagcg aggcctcggg cggcgccgtc gccggaccca
     8401 tcgccaagtc cgtcatggag gcggtgttga actcatgagc gcgaccccac gaagttcctc
     8461 ccacgcagcg cgtgtgaccc gccccgcctc gcttcgcctg attactgcgc gaggtccgct
     8521 catgaccagc tccccgaacc tccctagggg gcccgaatga ccatccatgt gcccaagctc
     8581 ctgggcggcc ggtacgaagt gggcgagctg atcgcccgcg ggggtatggc cgaggtccac
     8641 cacggctacg acacccgcct gggccgcacg gtcgccatca agatcctgcg cagcgatcac
     8701 gtccgcgacg cccacttcct ctcccgtttc cgccgggagg cgcagtcggt ggccgggctg
     8761 aaccaccgca atatcgtcgc cgtctacgac tccggcgagg accagctcac cgagaccggc
     8821 ggcgcacgcg tgccggtgcc gtggatcatc atggaatacg tcgacggcta caccctgcgg
     8881 gagttgctca acgagcatgg ggacctgccg ccgcaggagg ccgcccggat caccgagtcc
     8941 gtcctggacg ccctggccta ttcgcacgag atgggcatgg tgcaccgcga catcaagccc
     9001 gccaacgtca tggtggcggg cgacggcacc gtcaaggtca tggacttcgg catcgcccgc
     9061 gcggtcgccg acacccaggc gacgatgacg cacacctcct cggtcatggg cacggcgcag
     9121 tacatctccc ccgaacaggc cgagggcctg acggtcgact cccgctccga cctctactcg
     9181 accgggtgcc tgctcttcga actgctgacg gggcgcaccc cgttcgtcgg ggagccggtg
     9241 tcgctgacct atcagcacgt ctcgaagccg ccgccgctcg cctcctccct caacccggcg
     9301 gtctccgagg atctcgacgc cgtcatcgcc ctggccctga ccaaggaccg ggagcagcga
     9361 tatcaggagg cggaagactt ccgcgacgac ctgcgctcgg tgcggctggg acggccggtc
     9421 agcgcagcgg catacgcagc cctgcgcgcc gaccaaccca cggccgtcgt cgccccggcg
     9481 cagcacatcc cgccgacccc cgtcgacgtg ctggcgcccg agcagccgta tgacgagccg
     9541 cggcgccgcg ggtccggcgt gctcatcgcg tcgatcctgc tgctcgccgt gggggtgctg
     9601 gggttcgccg ccaagaccta tctggactcc cggcccgaat atgtgacggt gccgtccgtc
     9661 gtgggcaaga ccctgaccaa ggccagcagc gacctgcaag ccgccggctt cgaggtcagc
     9721 tcgtcctatc aaccctcgtc cgaatacgac aaggacaagg tgatggatca ggaccccaag
     9781 ggcggcaccg aacagctggc cggcaagcag atcaacctca ccgtgtccgg cggtccggcc
     9841 gcgacgcgcg tcccgccgct ggcggggctc acgctcgacg aggccaccgc ggcgttggag
     9901 gatgccgggc tcgaactcgg agatgtgcga tcgctcgact cccccgacgc caagaaggac
     9961 gaggtcgtct cctccacgcc ggaggagtat gccgacgccg ccgagggcga cgaggtcgac
    10021 ctgaccgtcg gcactgggaa ggtcgcgctg cccgacctgg tcggcaagac ccaggacgag
    10081 gcccgagcca ttctggcgga gttgaagctg ccggtcctga ccgtcctgga ggagagctcc
    10141 gaaaccgagg acacggtgct gcggaccaac cccaagaagg gcaaggtcaa cgtcggcacc
    10201 accgtgacct tgaccctggc gaagccgcgc acgatcgtgc agacgacgac gatcacgacg
    10261 acccagtcga gcaccccgcc gccgaccgaa ccgacgacat cgacggacac cgcgacgtcc
    10321 acctcgacca ccccgccccc gaccgacacg ggcaccggcg gcgggaccaa cacgggcaac
    10381 acgataccga ccatctccca gacgtccgcg cccccgctgc gcaactgacc atacgatcag
    10441 ccgatggcca aaatcctcgt cgtcgacaac tacgacagct tcgtcttcac gatcgtcggc
    10501 tacctgcagc aactcggcgc cgagacgacc gtggtgcgca atgacgcgat cacaccggac
    10561 gagggcaccg gcttcgacgg ggtgttgatc tcgcccgggc cgggcacacc tgaggaggcc
    10621 ggggtgtcga tggcgatgat cgccgcgtgt gccgaacgca gccagcccat gctgggggtg
    10681 tgcctggggc accaggccct cggggtggtc atgggggcga ccgtcggccg ggcgccggag
    10741 ttgttgcacg gcaagacgtc tcaggtgcac cacgacggga ccggtgtgct cgacgggctg
    10801 gcggatccgt tcacggcgac ccgataccac tccctgacga tcgacccgcc cacgctgccg
    10861 gactccctgg tcgccaacgg ccataccgac agcggcatca tcatggccgt ccgccaccgc
    10921 gacctgcccc tgcacggcgt gcagttccac cccgagagtg tcctgaccca tgggggccac
    10981 cgcctcctcg cgaactggct ggccgtatgc ggtgaccccg ccgccgtcgc cgccgccgag
    11041 ggcctgagcc cgctcgtccg ggacgccgcc ggcatcgccc gtgtcgtcgc cggctgaccc
    11101 cctccccgcg acgttggtgg ggcgaggcag cggttagtcg gcgacccggg cggaggtgag
    11161 ggagagggtg ccggcgaagg cggggaaggt cttgatgccg gtgcgctcga ccgcatagcc
    11221 caggccgagg gcactgacgt actcccgata gcgatcgatc gtcgggtcgg cgtccagggc
    11281 ggcctccagc gcggcgggat cgccgatcac gctgatcacg aacggcggtg agtagacgcg
    11341 gccctgcagg atcagggtgt tgccgacgca ccggacggcg ctggtcgaga tcacccgctg
    11401 gtccatgagc atcatcgcct cggcgccgcc gcgccacagg gcgttgacga cggcctgcac
    11461 gtcctgctgg tgcacgacga tgtcgtccac ggtcgcccat tcgggcagct gatcgctggt
    11521 caggggcgag tcgtcgaggg cgacggtgag ggtgtccccc tcgaccgcgg cggtgcccgc
    11581 gcccgcggcg agcgcgtcgg ccgcaccgga cagtttcgcg atccgctcac tcccgggagc
    11641 ggccgcggcc gtgagccggt cgacgtccgc gcggaccgcg gcgacctcgc ccactcggtc
    11701 ggccatcagg cgctgacgct cgcggatgac gtcggcgagt ccgggcgcgt ccgagcgcag
    11761 gtcggtgccg cgggaggcct gaccgctggt ggagaacagg atgcccgcga ggaccgccac
    11821 gatcggcacg agcagcgacc acatcgtcgg tcggcgggtc agcacgagcc agcgccgcca
    11881 ccgggaggcc gcgacccgcg cggccgaccg ccgggctgcg cgccggtccg gctgaggctc
    11941 ctgctcggtc atggtgcccc cacagtaggt cattcgcgca ggaccggctc gtgcgtcgac
    12001 taccctggag gccagttccg cgaatgacgc acagggagat ccgcacgtgc cagagtcgaa
    12061 gggccgcaag aaggcgacct acaccccgca ggaagcgccc cgcgcgcaga aggtcaagac
    12121 cggcaacccc tcgtggtacg tcccggtcat gctcggcctg atgatcctcg gcctgctgtg
    12181 ggtcgtgacc ttctacatca ccaagcagca gtaccccatc gaggccctcg gccgctggaa
    12241 cctcggcgtc ggcttcgccc tgatgctcgc cggcttcggc atgaccacca actggcggta
    12301 agccgccgcc gcgctcgatc ggggcgaccg ttcagtcgtc ggcatggtcc gctcactcga
    12361 gctcacgaca agcgtcacgg cgcaactaag gcccgcttgt cctcagttgt ccccagggtt
    12421 atccccggac tgtgcgttat ccacccagtt gtccacagct ggggacgaat gacaccggtg
    12481 tgattcccgg gggatggtat gccgcccggt ccggcgactc ggaaaacggt cgcctcggaa
    12541 agcggcctca gccggtcagg tagcgcgcca cgccggcggc gacgatcgcg ccggtgacgg
    12601 ccgcggcgcc gccgtacgcc accgccgcat tgcgccggcc gcgcgcgaag acgaacgcca
    12661 ggagcaggcc ggtgaggaag ccgccgacgt gcgcctgcca ggcgatattc ggatagagca
    12721 gcggcagggc agcattgatc gcgaggttga gcatgatgcc ctgggtgctg cgcccgaggg
    12781 cccggttgag gactatcaag gcgccgaaca gcccgaacac cgcgcccgag gcaccgacga
    12841 ccccgccggt cgcggtcacc gggttgagca agatgtaggc caccccgccg ccgatggcgc
    12901 tgaccaggta gaggatcagg aagcgcacct tgcccagcag cggctccaga taggagccca
    12961 gcatccacag ggcgaacatg ttgaacagga tgtgcatcgg cggcccgtgc aggaaggccg
    13021 aggtgacgaa ccgccacggc tcctcctccc cgatggccgg ggagagcgcg aaccggtaga
    13081 agatctcgtc gctgcggctg gcctgctcca gcagccagat gcccacgcac gcggcgatga
    13141 ggatcagcgt gacgatcggc cgaccgcccc ggtcccccgt ccgggcaccg aggaccgtct
    13201 tggtcgtcgg gtgctcccgc gccgattggg cgacgcacga aacacactgc acccccacgg
    13261 cagcggcccg ctggcactcg gggcatgtgg ggttgccgca ccgctggcag cggacatacg
    13321 agatccggtc ggggtggcgg ggacacacgg gcgcgggggc gccgggaccg gcatacggct
    13381 cggtcatccg gcgctccccg gcgttcccgg caacgggcgc ggcatagggg tcacgcggtc
    13441 aataacgcgg acggactcag gagagttcga ccgactcgat gacgaccggc tcgaccggct
    13501 tgtcgttggg cccggtcggc acggcggcga tcgcgtcgac gacctggcgc gaagcgtcgt
    13561 cgctgacctc accgaagatc gtgtgcttgc cctgcagcca cggcgtgggc tcgacggtga
    13621 tgaagaactg ggagccgttg gtgcccttgc ccatgcgctt gccggcgttg gccatggcga
    13681 gcaggtaggg ccggtcgaac gccagctcgg ggtggatctc gtcgtcgaag gtgtagcccg
    13741 ggccgccgat gccctggccg agcgggcagc cgccctggat catgaagccg gggatgatgc
    13801 gatggaatcc gagcccgtcg aagaacttcg tcgggttgga gcgcccggcg tcgtccttgt
    13861 actccttctc gccggaggcg aggccggtga agttggcgac ggtcttgggg gcctggttgt
    13921 cgaagagttc gaccacgatg tcgccgaggt tggtgtgcag ggttgccttc atggcgccaa
    13981 gtcaatcacg cgttccctgt gagttccctg ggagcggtca cttcgtcggc ccctaacgac
    14041 gcatcgcccg tgtggaggag gcaggatgga ggccagatac gaccgcatcc cgaaggagag
    14101 accatgctgt gcagcaagcc cccgtcgaag accgagaagg ccaaggccgc ggtcgccgac
    14161 tacgtcaaca ccgccgcgga gaaggccgcc gagatgcgcg aggaagcagc tgaccgggcc
    14221 tcacaccgtg cgtcgtatgt cgcggaccag tccgccgacg cccgcgccgc cgccgcgcac
    14281 cgcgccgcgg agttgcgtga ccgggccgcc gccgcgagtg agaaggccgg cgagcgcgcc
    14341 tcgcacctga tggagcgcgc cgccccggcc gtggatgtcg ccaaggagcg ggccgctgcc
    14401 gctcgcgagg cggccgccca cgggtatgac gccgccgcac ctcgtgtcga aaccgcccgc
    14461 aacacgctgg tcgaggatgt cctgccgaag gtcgccgggg ccatcgccac cgtcgccgcg
    14521 ggcgcgctgt cggccaagga ccaggccgcc gagaccgcgc accgggcgcc ggaggcgttc
    14581 gcggtgctca agggtgaggc gacggcgaag ccgaagaagc gctcgtgggg tccgttcctg
    14641 ttgctcgccg ccctcgccgg cggcgcggca tacgtcctga cgcgccgcaa gacccccgcc
    14701 accgacccct gggcggtgcc cgccagcgac ccgtatgccg cgcgctcgcc gatggcgggc
    14761 gacaccggtt cctcgttgca ggacaaggcg gcgtcggtcg ccgccgcggc gaaggacaag
    14821 gccgccgagg tcaaggacac cgtcgccgac aaggctgcgg aggtcaagga caaggcgagc
    14881 gacaaggccg ccgaactcaa ggacaaggcc ggcgacgccg cggacgacgc gaaggacgcc
    14941 acctccgacg cgttcgacga cgccaagggc caggtctccg aggcacagga tgccgccggt
    15001 gacgccctcg acgagaccgg cgacaacctc ggcgaccgca tcgagaacgc caccgacacc
    15061 gccgccgact ccatcaacga cgcggccgac cgtgtcgagg acgccggcga ccggaccaag
    15121 gacaactggg gctgagccca gcacctgcaa gcccagcccc tgcaagccca acacccccgc
    15181 aagcccaaca cccccgcaag acc
//
LOCUS       DAOPJG010000067        15099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4137_length_15099_cov_7.389258, whole genome shotgun sequence.
ACCESSION   DAOPJG010000067 DAOPJG010000000
VERSION     DAOPJG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4137_length_15099_cov_7.389258"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PKU75_07990"
     CDS             <1..761
                     /locus_tag="PKU75_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HPF80854.1"
                     /translation="AADVAAAEKIFDKALADRGLHLNASGRAVDADGKAAPAVTVPVY
                     FNIITAGTKGAVTQTRVSKQMTVLNNAYSTAGFRFKLMGTKRVNNSGWFNLSPGSSAE
                     RSMKTALRKGGTNTLNVYTANLSGGLLGWATFPSDGAGSKYDGVVLLHSSLPGGSATN
                     YNLGDTATHEVGHWMGLWHTFQGGCGGAGDQVADTPAESSPAFGCPTGRDTCSAPGKD
                     PIRNFMDYTYDSCMNMFTAGQKTRMKNQWAAFRG"
     gene            complement(837..1325)
                     /locus_tag="PKU75_07995"
     CDS             complement(837..1325)
                     /locus_tag="PKU75_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPF80855.1"
                     /translation="MAPSSSVAIDELDARLITVFTENPDVGVLGASRILGVARGTVQA
                     RLERLEQRGVIRSFAPTLDPAALGFPVTAFCMLEIRQRQGHSPVVDHLKLIPEVLEVH
                     SITGTGDLLVRVVAKDNADLGRVIDTVLDEVHVIRANTSISLVTHLEHRTGPLLTRSL
                     DG"
     gene            1436..2656
                     /gene="hppD"
                     /locus_tag="PKU75_08000"
     CDS             1436..2656
                     /gene="hppD"
                     /locus_tag="PKU75_08000"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HPF80856.1"
                     /translation="MSDTAMTLTEAEREAQLNLEQLKQLVGLVEYAADRDPFPVTGWD
                     AIVFVVGNATQAAHYYQSAWGMELVAYSGPENGNRDHKAFVLKSGSIKFVLKGAVSPD
                     SPLIAHHAKHGDGVVDIALEVPDVDKCIAQAKAAGATVLAEPYEMSDEHGTVRLAAIA
                     TYGETRHTLVQREVGGVRYAGPHVPGYVARSSTFVKRESAPKRLFQALDHIVGNVELG
                     KMDEWVAFYNKVMGFVNMAEFVGDDIATEYSALMSKVVANGNHRVKFPLNEPAIAKKR
                     SQIDEYLDFYTGPGAQHLALATNDILRTVDELTKEGVEFLDTPDAYYDDPELRARIGQ
                     VRVPIEELKSRKILVDRDEDGYLLQIFTKPLGDRPTVFFEIIERHGSLGFGKGNFKAL
                     FESIEREQDKRGNL"
     gene            2727..4526
                     /locus_tag="PKU75_08005"
     CDS             2727..4526
                     /locus_tag="PKU75_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPF80857.1"
                     /translation="MTATRRQLLAMSVVGGAGLFAAEPSAAAVKNLRLTVLGTTDLHG
                     NVYNWDYFKNQEYDDRYGNDIGIAKAATLIKAMRAERGPRRCIVLDAGDTIQGTPLAY
                     YYAKIDPITGGSKHPMALAMNAIRYDAAALGNHEFNYGMDTLRAFQRQLDHPLLGANA
                     VGWTSGKPVFPPYILKRITLPDTKTITVGILGLVTPGVAIWDKANVEGRVKFPGLVEQ
                     ASYYVPRMRAAGAEVVVISAHSGPNTSSSYGDALPYPENAASLVAEQVPGVDAILVGH
                     AHLEVPERFVTNTATGRQVLLSEPLYWGMRVTVMDLYLSLVRGKWQVRTARATLLNAN
                     TVPEDPRVASLVRGAHEKVLTYVNSPIGTCTQAMSAAGSRYEDTAAMDFINLVQAEAV
                     KAGIAGTAAEGLPVLSIAAPFNRLAAIPAGQVTVRDVAGLYIYDNTLLGIQFSGAQVR
                     AYLEQSAVYFKQVPGPGTYSADEVTNAPTETAPGGTPDYNYDIMGGLDAPLTYDIDIS
                     VPPGNRIKNLAYDGVPVSDSQQFVIAINNYRQSGGGGFPGVTTAPVVYNRQNEIRQLL
                     IDWVSAEGTIDPPSFFRVDWRLVAGDTPLVITP"
     gene            complement(4536..5123)
                     /locus_tag="PKU75_08010"
     CDS             complement(4536..5123)
                     /locus_tag="PKU75_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF80858.1"
                     /translation="MSPRRLRFLTHPQVRVDPATPVPQWDLSDVGRARAAALAGSPFL
                     AATTSIWSSTEVKALRTAELIAGGIPHLVREDLGENDRSATGFVPPEEFEALADAFFA
                     HPDESVRGWANAADEQRRIVAAVDAVLTDERSGDGDIVVVAHGGVGTLLLCHLLGRPI
                     TRELDQPGQGHYFTVDRDTRAVQHGWHPLEELSAE"
     gene            complement(5207..8794)
                     /locus_tag="PKU75_08015"
     CDS             complement(5207..8794)
                     /locus_tag="PKU75_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80859.1"
                     /translation="MSSNVIESSAISVDNPPLPSDPRRTVTWRRVRTPLNMVALSVLL
                     VGSVAQVFGNVLAGQIAEAPTWELVRWLAIVMVGGSVLDMAGRYVWFGEIDRVEGGLR
                     ADLLDAALNQPLAALGEQAVGEVLDRVDDDTREVGNLMRWQSWMVLRSLFSAVPMWVI
                     GGLIWWPAWFMFPVFALLTWLGMRSLLRPIAERKVIEEAAWTDHAAALEEGIAGRDDL
                     RTSLGQSFAIRRLAQLSAQVHARFLAVITLEKKIVLRGVLVLNALLAAVASIGVAMAS
                     SGRASVAQLVTIFLITSQFVGVVGFVAEQLPDIQAGLGAVTRLRQMLALAPEPVGGRR
                     IEAEHVDVRFDHVDFAYEEGTFALSDIDLHVPAGQTLALVGRSGSGKSTLASLLSRAV
                     DPAPGTVFVGGIDVLDLDLQSLRRQVGVVTQRTEILAGTLAQNIALFADLPRERVEGV
                     VTELGLNDWVAGLEHGLDTVLGPGGTTLSAGEEQLVAFARLLIRDVHVVVLDEATARM
                     DPLTEARVVAAADRLLSGRTGLLVAHRLSTIARADLVAVLSAGRLVQFGAQARLATID
                     GPFRDLLRSATLDEPGVAPSGTEPGAPHLSQDPDGTVPGATVDAPADGTSESVGSARR
                     RRAALDRPETGTGPSLARGITSVLRVRPGWGLLAVVLFLGMSLTGGAGALTGFAWGHT
                     VERIGRGEDPWWWLAGVVFMVLVGPLMLAEAISRYPRWWVEVLLRVRMAVLVGQTAQR
                     RLEKVPAGEVVARAMDSDRYARYADRWVDFVNGLIVVAVTAVAAANPLAGLVLLGVVV
                     AATLTSAIGRPIAGRTATKASAMRARFGRALVSTLESARTVKLAGRTPELHRHLQRVD
                     AGRVDAAVREHHVQAVLDSVPTIMIKVGVVVVWAAVVENWWGLATALLVANALNGFEW
                     FGRVAGAVVTEAPGTRSWQAATSQMAGGVDLMRVPPGVDLVTGDAPPAPEVERVPLRE
                     LIVDDVTAIHEDGTVGVEDVSLSIRRGELVLLLGQVGSGKSSLLRTLAGLIDYEGRVL
                     WNGQVVGDAELFMRPGQVAYVAQVPRVLSGTFADNVQLGFGRAFDAPVADARLTPDVT
                     AAGGATALVGHRGVRLSGGQVQRLALARALAADAELLLADDVSSALDAKTEVELWEAL
                     RRRSATVVGATSKRAALARADRVVVLVDGRVEAVGPWSRLSGDWGHLAG"
     gene            8858..9451
                     /locus_tag="PKU75_08020"
     CDS             8858..9451
                     /locus_tag="PKU75_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF80860.1"
                     /translation="MLPTHLDLTGRTAIVTGAGSPTGIGYAAATLLAGLGASVVLGST
                     TERVQRWRASLARNLDTAYLVCRAALPHLRASGSGRIVNVTSVTGPVMAMRGEIAYAT
                     AKAGMVGLTRALAVDEGPHGVTVNAVAPGWIETGSQTPDEAEEGRHTPVGRSGTAGEV
                     ASAIAWLCTPGAAYVTGQVIVVDGGNAIAEERLVATP"
     gene            complement(9448..9796)
                     /locus_tag="PKU75_08025"
                     /pseudo
     CDS             complement(9448..9796)
                     /locus_tag="PKU75_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(9844..11178)
                     /gene="gabT"
                     /locus_tag="PKU75_08030"
     CDS             complement(9844..11178)
                     /gene="gabT"
                     /locus_tag="PKU75_08030"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HPF80861.1"
                     /translation="MIEQRRILTTAIPGPKSQELQQRKLASVSTGVSTGLPIYVEAGA
                     GGILRDVDGNQLIDFGSGIAVTTVGNGNQRVIDAVSKQIADFTHTCFMVTPYEEYVQV
                     CEELDKRTPGDHEKRSALFNSGAEAVENAVKVARHFTGKDGIVAFDHAYHGRTNLTLA
                     MTAKNMPYKHKFGPFASELYRVPMAYPYRWPGGPEHCEAEAFNYITDQIHAQIGEENT
                     AAVIIEPIQGEGGFIVPPPGFLPALAQWCRDKGILFIADEVQSGFCRSGDWFAVDHEQ
                     VVPDIVVTAKGIAGGMPLSGITGRADIMDTIHPGGLGGTYGGNPVACAAALASIETME
                     EDDLCGRAKAIEAILYERLGRIAEKYEQVGEVRGRGAMIAMELVTDRESKEPAAALTG
                     AINKACHERGLVTLTCGTLGNVFRFLPPLTIGDDLLHEGMDILEEAFSVAAG"
     gene            complement(11212..12438)
                     /locus_tag="PKU75_08035"
     CDS             complement(11212..12438)
                     /locus_tag="PKU75_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF80862.1"
                     /translation="MTLHSPQDAPEPRARIPREIQVLIAAAFVIAVGFGLITPVLPAF
                     ATSFDVGATAASIVVSAFAFFRLVGAPGGGRLVARFGERPIYLLGLVIVAISSAATAF
                     AHNYWQLLAYRGLGGLGSTMFTISAVALLARLAPPAIRGRVSSAYGAAFLIGGVAGPA
                     IGGLLGGLGLRVPFLAYAVCILLAAALVWAMLPKDAGRPVADGTARPAVTMRECLPDP
                     AYRAALGSFFANGWANFGVRVAILPLFAAAVVSDQPWVAGLALAVFAVGNMLGLAWAG
                     RRSDVRGRKPFVIGGLAVSGFATLWTGFAVTIPLLVGLCLIIGVGAGTMTPAQQAAAA
                     DIVGQERSGGPALAALQMAQDAGGILGPIAAGALVDLSGGYHWPFVVTGLVTLVAILP
                     WLRARETSPAARAAYP"
     gene            12491..13465
                     /locus_tag="PKU75_08040"
     CDS             12491..13465
                     /locus_tag="PKU75_08040"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPF80863.1"
                     /translation="MTTPSAPGWYDDPEAPDQLRYFDGIVWTRHTTPRSTRAGAGAAT
                     PQAGAPVAPQPQQQPTGTADPYGWRGAGSQQGQPPQQPDQYGQQPQWSPPRQDQAPQW
                     RPPQQQWGQPQQQWGQQAPGWGPAAGQVGPATPDGVPLAGYWQRVGAYILDLLIVGAL
                     TAICGGYWLLKAIRPWLDQVTDAINSSTTDPQRLEDLSNSLSTYLTGRDFMIFTAISL
                     LVGLIYHVFFLTRTGATPGKMALRISVRERERPGPLPVQVALRRYLLTFVVNVLGWVP
                     GLLSVLAGLVGIADLLWPAWDPKRQALHDKIAGTNVVRGKQPRTPANS"
     gene            complement(13554..14759)
                     /gene="fahA"
                     /locus_tag="PKU75_08045"
     CDS             complement(13554..14759)
                     /gene="fahA"
                     /locus_tag="PKU75_08045"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HPF80864.1"
                     /translation="MSWWRDLPADEPFGLANLPYGVADLSDGPRVVVRVADHALAIGG
                     LAERAGMESGAVWQCGSLNPFLDQGPQAWASARTWLQETLASAEYDDCVGANLHPLDG
                     VALLLPITVADYVDFYASEHHATNVGRMFRPDQAPLTPNWKHLPIGYHGRSGTIVVTG
                     TDIVRPCGQRKPPEADAPVFGPSVRLDIEAELGFVVGGATTLGEPVPLEQAWDHIFGV
                     TILNDWSARDLQAWEYVPLGPFLGKSFATSIGAWITPTAALAEAKVALPGQDPALLPY
                     LTGAAAYGLDIHYEVEWNGTVVSRPEYRDMYYSPAQMLTHMTVNGATLRSGDIFGSGT
                     ISGPEKETRGCFLELSWSGQEPITLDAGSTRTFLEDGDTVILRATAPGANGAVIGLGE
                     CVGRVLPAR"
     gene            complement(14756..>15099)
                     /locus_tag="PKU75_08050"
     CDS             complement(14756..>15099)
                     /locus_tag="PKU75_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80865.1"
                     /translation="GAIAMEVPLKLTGGLHHAVRGSYPEAPGRNPQDMHGVLNVLAAF
                     AAARAGRPVSAIQATLEQRDPGPLIAGLSALNPVSAARLRGDFPSYGCCTVTDPITEL
                     ITLDVLEGPRS"
BASE COUNT         2463 a         5103 c         5412 g         2121 t
ORIGIN      
        1 ccgccgctga tgtcgcggcg gcagagaaga tcttcgacaa agccctggcc gaccgtggtc
       61 tgcacctgaa tgcgtccggg cgcgcggtcg acgcggacgg caaggccgct ccggcggtca
      121 cggtgccggt gtacttcaac atcatcactg ccggcacgaa gggcgctgtg acccagaccc
      181 gagtctcgaa gcagatgacg gtcctcaaca acgcctacag cacggccggc ttcaggttca
      241 agctcatggg gaccaagcgg gtcaacaact cggggtggtt caacctcagc ccgggctcgt
      301 cggccgagcg cagcatgaag actgccctgc gcaagggcgg caccaacacc ctcaacgtct
      361 acacggccaa cctcagcggc ggcctgctcg gctgggcgac gttcccctcg gacggcgcgg
      421 ggagcaagta cgacggcgtg gtcctgctgc actccagcct cccgggcggt tcggcgacca
      481 actacaacct cggcgacacc gccacccacg aggtcggtca ctggatgggc ctgtggcaca
      541 ccttccaggg cggttgcggc ggcgccggcg accaggtcgc ggacaccccg gccgagtcgt
      601 ccccggcctt cggctgcccg accggtcggg acacctgctc ggccccgggc aaggacccca
      661 tcaggaactt catggactac acctatgact cgtgcatgaa catgttcacc gccggtcaga
      721 agacccgcat gaagaaccag tgggcggcct tccgcggctg atcgcccccg caagaacccg
      781 aatgggccgg tgtcgcagcg cgacaccggc ccattcagcg ttgacggtcg gggagctcaa
      841 ccgtcgagac tgcgggtgag taggggcccg gtccggtgct cgaggtgggt gacaagcgag
      901 atggaggtgt tggcccggat gacatgcacc tcgtccagga cggtatcgat gacccgcccg
      961 aggtcggcgt tatccttggc gacgacccgg accagcaggt caccggtgcc ggtgatcgaa
     1021 tggacctcga gcacctccgg gatcagcttg aggtgatcga cgacggggga gtggccctgg
     1081 cgctgacgga tctccagcat gcagaaggcc gtgacgggga acccgagggc cgccgggtcg
     1141 agggtgggcg cgaaagagcg gatcacgcca cgctgttcga gccgttccag acgggcttgg
     1201 accgtgcccc gggctacgcc caggatgcgc gaggcaccga gcaccccgac atcggggttc
     1261 tcggtgaaaa ccgtgatcaa gcgggcgtcg agctcgtcga tggccacgga ggacgaggga
     1321 gccaccccac aattctgaca gcttgatcgg tcaatttgct acgtagttgc acaaattgag
     1381 taacactact catgctggtt gcacacctcg ccgggtgtcg gacacccttt cgagcatgtc
     1441 ggataccgca atgacgctga ccgaggccga gcgcgaggcc caattgaatc tcgagcagct
     1501 caagcagctg gtcgggctgg tggagtatgc cgcggaccgt gatccgttcc cggtgaccgg
     1561 gtgggacgcc atcgtgttcg tggtgggcaa tgccactcag gcggcgcact attaccagtc
     1621 ggcgtggggg atggagttgg tggcctattc cggtccggag aacggcaacc gggatcacaa
     1681 ggcgttcgtg ctgaagtcgg ggtcgatcaa gttcgtcctc aagggtgcgg tcagcccgga
     1741 cagtccgttg atcgcccatc acgccaagca tggtgacggg gtggtcgaca tcgctctcga
     1801 ggttccggat gtcgacaagt gcatcgcgca ggccaaggcg gctggggcga cggtgctcgc
     1861 ggagccgtat gagatgtccg acgagcacgg gaccgtgcgc ctggcggcga tcgcgacgta
     1921 tggggagacg cggcataccc tcgtccagcg ggaggtgggt ggggtgcggt atgccgggcc
     1981 gcacgtgccg gggtatgtcg cgcggtcctc gacgttcgtc aagcgcgaga gcgcgccgaa
     2041 gcggctgttc caggccctgg atcacatcgt cgggaacgtc gagttgggca agatggacga
     2101 gtgggtggcg ttctacaaca aggtcatggg ctttgtgaac atggccgagt tcgtcggcga
     2161 cgatatcgcc accgagtact ccgcgctgat gtcgaaggtc gtcgcgaacg gcaaccaccg
     2221 ggtgaagttc ccgctgaacg agccggcgat cgccaagaag cgcagccaga tcgacgaata
     2281 cctcgacttc tacaccggcc cgggcgctca gcacctggcg ctggcgacca atgacatcct
     2341 gcgcacggtg gatgagctga ccaaggaggg ggtggagttc ctcgacaccc cggacgccta
     2401 ttacgacgac cccgaactgc gcgcccgcat cgggcaggtc cgggtgccga tcgaggagtt
     2461 gaagtcgcgc aagatcctcg tcgaccggga cgaggacggc tacctgctgc agatcttcac
     2521 caagcccctc ggcgaccggc ccacggtctt cttcgagatc atcgaacgcc acggctccct
     2581 cggcttcggc aagggcaact tcaaggcgct cttcgaatcc atcgaacgcg aacaggacaa
     2641 acgcgggaat ttgtgaccgc ccagggggca ccgaggcccg ggcaggtcta ccgtcaggtg
     2701 acatgggaga actccacgct gaccgtttga ccgccacccg ccggcaactg ctggccatgt
     2761 ccgtcgtggg aggtgccggc ctgttcgccg ccgagccgtc ggcggcagcg gtcaagaatc
     2821 tgcgcctgac cgtgctcggg accaccgacc tgcacggcaa tgtctacaac tgggactact
     2881 tcaagaatca ggagtatgac gaccgctacg gcaacgacat cggcatcgcc aaggcggcga
     2941 cgctgatcaa ggccatgcgc gccgagcgcg gaccgcggcg gtgcatcgtc ctggacgccg
     3001 gggacaccat ccagggcacg ccgctggcct actactacgc caagatcgac ccgatcaccg
     3061 gcgggtcgaa gcacccgatg gcgttggcga tgaacgcgat ccgctacgac gcggccgctc
     3121 tcggcaacca cgagttcaac tacgggatgg acacactgcg ggccttccag cgccaactcg
     3181 accacccgct gctcggcgcc aacgccgtcg gctggacctc cggcaaaccg gtgttcccgc
     3241 catacatcct gaagcggatc acgctgcccg acaccaaaac gatcaccgtg ggcatcctcg
     3301 gcctggtcac accgggcgtc gcgatctggg acaaggccaa tgtcgagggg cgggtgaagt
     3361 tccccggact cgtggagcag gcgagctact acgtgccgcg gatgcgagcc gcgggagccg
     3421 aggtcgtcgt catctccgcg cattcaggcc cgaacacttc cagttcgtat ggcgatgccc
     3481 tgccctatcc cgagaacgct gcctccctcg tcgccgagca ggtgcccggc gtcgacgcga
     3541 tcctcgtcgg ccacgcgcac ctggaggtgc cggagcgctt cgtgaccaac acggccaccg
     3601 gccggcaggt cctgctctcc gagccgctct attggggcat gcgggtgacg gtcatggacc
     3661 tctatctctc gctcgtcagg ggcaagtggc aggtccgcac tgcgcgcgcc accctgctca
     3721 acgccaacac ggtgcccgag gacccgcgcg tggcgtcgct ggtgcgcggc gcgcacgaga
     3781 aggtgctgac ctatgtcaac agccccatcg gcacgtgcac gcaggcgatg agcgccgccg
     3841 gctcacgcta tgaggacacg gcggcgatgg acttcatcaa cctggtccag gccgaggcgg
     3901 tcaaggccgg aatcgccggc acggccgccg aggggttgcc ggtgctctcg atcgccgcac
     3961 ccttcaaccg actcgccgcg atccccgctg ggcaggtgac ggtgcgcgac gtcgcgggcc
     4021 tgtacatcta cgacaacacc ctcctgggga tccagttcag tggcgcgcag gtcagggcct
     4081 acctggaaca gtccgccgtc tatttcaagc aggtcccggg gccgggcacc tattccgcgg
     4141 acgaggtgac gaatgctccc acggagaccg cgcctggagg aacgccggac tataactacg
     4201 acatcatggg tgggctcgac gcgcccctca cctatgacat cgacatctcc gtcccgccag
     4261 gcaaccggat caagaacctc gcgtacgacg gtgtgccggt gagtgattcg caacagttcg
     4321 tgatcgcgat caacaactac cggcaatccg gtggtggcgg cttcccgggt gtcacgaccg
     4381 ccccggtggt ctacaaccgg cagaacgaga tccggcagct gctcatcgac tgggtgagcg
     4441 ccgagggcac gatcgacccg ccgagcttct tccgggtcga ctggcgcctc gtcgccggcg
     4501 acaccccgtt ggtcatcacg ccctgacgcg catcctcatt cggctgagag ctcctcaagt
     4561 gggtgccatc cgtgctggac ggcgcgggtg tcccggtcga cggtgaagta gtggccctgg
     4621 ccgggctggt cgagttcgcg ggtgatgggc cggccgagga ggtggcacag caggagggtg
     4681 ccgacgccgc cgtgggcgac gacgacgatg tcgccgtcgc cggatcgctc atcggtgagc
     4741 acagcgtcga cggcagcgac gatccgccgc tgctcgtcgg ccgcgttcgc ccagccgcgc
     4801 accgactcgt cgggatgggc gaagaaggcg tcggccagtg cctcgaactc ctctggcggc
     4861 acgaatcccg ttgcgctccg gtcgttctcg cccagatcct cgcgcaccag gtgcggtatg
     4921 ccgcccgcga tcagttcggc ggtccggagc gccttgacct cggtggagga ccagatcgac
     4981 gtcgtggcgg cgaggaacgg cgagcctgcc agcgccgccg ctcgcgcgcg accgacgtcg
     5041 gacaggtccc actggggcac gggagtcgcg ggatcgacgc gcacctgggg gtgagtgagg
     5101 aatcgcaggc ggcgcgggct catggcaccc gtcctacacg atgccttctc ggctcgccaa
     5161 gcgcgtcttg cgatgctgcg cccacacctc ggccggcgtg atggcctcag ccggcgagat
     5221 ggccccagtc gcccgacagc cgcgaccacg gcccgacggc ctcgacccgg ccgtcgacga
     5281 gcacgacgac ccggtcggcc cgggccagcg ccgcccgctt ggacgtggca ccgaccaccg
     5341 tggcggaccg gcggcgtagc gcctcccaca gttcgacctc ggtcttggcg tcgagcgcgc
     5401 tcgagacgtc gtcggccagc agcagttcgg cgtccgccgc gagggcgcgc gccagcgcca
     5461 agcgttgcac ctgcccgccg gacagccgca ccccgcggtg gccgacgagg gcggtggcgc
     5521 caccggcggc ggtcacgtcc ggcgtgagtc gggcatcggc gacgggggcg tcgaaggccc
     5581 ggccgaaccc gagttgcaca ttgtcggcga aggtaccgga caggacacgc ggcacctgcg
     5641 ccacgtaggc gacctgtccg ggcctcatga acaactcggc atcgcccacg acctggccgt
     5701 tccacaggac acgaccctcg tagtcgatga ggccggcgag cgtgcgcagc aggctggact
     5761 tgccggaccc cacctgcccc agcagcaaca cgagttcgcc gcgccggatc gacagggata
     5821 cgtcctcgac gccgaccgtg ccgtcctcgt ggatggccgt cacgtcgtcg acgatgagtt
     5881 ctcgcaacgg aacccgctcg acctccggtg ccggtggcgc gtcaccggtg accagatcca
     5941 ctcccggcgg gactcgcatc aggtcgaccc cgccggccat ctggctcgtg gccgcctgcc
     6001 acgagcgggt gcccggggcc tcggtgacga cggcgccggc aacccggccg aaccactcga
     6061 agccgttgag cgcgttggcg acgagcagtg cggtcgccag gccccaccag ttctctacga
     6121 ccgccgccca cacgacgacg accccgacct tgatcatgat cgtcggcacg gagtcgagca
     6181 cggcctgcac gtggtgctcg cgcaccgcgg catccacgcg gccggcatcg acccgttgca
     6241 gatgacgatg cagctccggc gtgcgcccgg cgagcttgac cgtgcgcgcc gactcgagag
     6301 tggagacgag cgcgcggccg aaacgcgccc gcatggccga agccttggtt gccgtccggc
     6361 cggcgatcgg ccgcccgatc gcggaggtca gcgtggccgc cacgacgacc ccgagcagca
     6421 cgaggccagc caggggatta gccgcagcga cggcggtgac ggccacgacg atcaggccgt
     6481 tgacgaagtc gacccagcgg tcggcatacc gggcgtagcg atcggagtcc atggcccggg
     6541 ccaccacttc gccggccggc accttctcga gtcggcgctg ggcggtctga ccgacgagca
     6601 ccgccatccg cacgcgcagc agcacctcga cccaccaccg tgggtagcgc gagatggcct
     6661 cggcgagcat cagcggcccg accagcacca tgaacacgac accagcgagc caccaccacg
     6721 gatcctcgcc gcggccgatg cgttcgacgg tgtggcccca ggcgaacccg gtgagcgctc
     6781 ccgcgccccc ggtcagcgac atgccgagga agagcacgac ggcgagcagc ccccagcccg
     6841 ggcgcacgcg gagcacactg gtgatcccgc gggcgagcga gggaccggtc ccggtctccg
     6901 ggcggtccag ggccgcgcgc cgacgccggg ccgacccgac ggactctgag gtcccgtcgg
     6961 ccggcgcgtc cacagtggct cccggaaccg tgccgtccgg gtcttggctg aggtgcgggg
     7021 ctcccggctc cgtcccggac ggggcgaccc ccggctcgtc gagggtggcc gagcgcagca
     7081 agtcgcggaa ggggccatcg atcgtggcga ggcgggcctg cgccccgaac tggacgagac
     7141 ggccggcgga gagcaccgcg accaggtcgg cccgggcgat cgtcgagagg cggtgggcga
     7201 cgagtaggcc ggtgcggccg ctgagcagcc ggtcggcggc ggcgacgacc cgggcctcgg
     7261 tgagcgggtc catgcgggcc gtggcctcgt cgaggacgac gacgtggacg tcgcggatca
     7321 gcagccgggc gaaggcgacg agttgctcct cgccggccga cagcgtggtc ccgcccgggc
     7381 cgagcacggt gtcgaggccg tgttcgaggc cggcgaccca gtcgttcagg cccagctcgg
     7441 tcacgacccc ctcgacgcgc tcgcggggga ggtcggcgaa gagcgcgatg ttctgggcca
     7501 acgtgcctgc gaggatctcg gtgcgctggg tcacgacgcc gacctgacgg cgcaacgact
     7561 gcaggtcgag gtcgagcaca tcgatgccgc cgacgaacac cgtgcccggt gccgggtcga
     7621 ccgcccgcga gagcagcgag gccagcgtcg acttgccgga gccgctccgg cccaccaggg
     7681 ccagcgtctg tccggcgggg acgtgcagat cgatgtccga cagggcgaag gtgccctcct
     7741 cgtaggcgaa gtccacgtgg tcgaaacgca catcgacgtg ttcggcctcg atgcgtcgcc
     7801 cgccgactgg ctcgggggca agcgcgagca tctgccggag ccgggtcacg gcccccagac
     7861 ccgcctggat gtccggcagc tgttcggcca cgaagccgac gacgccgacg aactggctcg
     7921 tgatcaagaa gatcgtcacg agctgggcga ccgacgccct tccgctgctg gccatggcga
     7981 cgccgatgct cgcgaccgcg gccagcaggg cgttgagcac gagcacaccc cgcagcacga
     8041 tcttcttctc gagggtgatg accgcgagga atctggcgtg gacctgagcc gagagctgcg
     8101 ccagccggcg gatcgcgaag gactggccga ggctggtgcg caggtcgtcc cggccggcga
     8161 tgccctcctc gagcgctgcg gcgtgatcgg tccaggccgc ttcctcgatg accttgcgtt
     8221 cggcgatggg ccgcagcagc gagcgcatgc ccagccacgt gaggagggcg aacaccggga
     8281 acatgaacca cgccggccac cagatcaggc cgccgatgac ccacatgggc acggcgctga
     8341 acagcgagcg cagcaccatc cacgactgcc agcgcatgag attgccgacc tcgcgggtgt
     8401 cgtcgtcgac gcggtcgagc acctcaccga cggcctgctc gccaagggcg gcgaggggct
     8461 ggttgagtgc ggcatcgagc aggtcggccc gcaacccgcc ctcgacccgg tcgatctcgc
     8521 cgaaccagac gtaacggccg gccatgtcga gcacggaccc accgaccatc acgatggcga
     8581 gccaccggac cagctcccac gtcggcgcct cggcgatctg cccggcaagg acattgccga
     8641 agacctgcgc caccgagccg accaacagca cggacagggc aaccatgttc aacggcgtcc
     8701 tgacccgtcg ccacgtcacc gtccggcgcg ggtcggacgg caggggagga ttgtcgaccg
     8761 agatggccga ggactcgatg acgttgctgc tcatggcaat caggcagcct acggcgcacc
     8821 cccgacagtc cgcgcgcgaa tatctatgct gaggcccgtg ctccccaccc atctggacct
     8881 gaccggacgc accgccatcg tcaccggcgc cgggtcaccg accgggatcg ggtatgcggc
     8941 cgccaccctt ctcgccggac tcggagcgag cgtggtgctc gggtcgacga cggagcgggt
     9001 ccagcggtgg cgggcgagct tggcgcggaa tctggacacg gcatacctcg tgtgccgcgc
     9061 ggcgctgccg cacctgcgtg cgagcggctc cggacggatc gtcaatgtca ccagcgtcac
     9121 cggcccggtg atggccatgc gcggcgaaat cgcctatgcc acagcgaaag ccggcatggt
     9181 cggcctgacc cgggcgctgg cggtcgacga ggggccgcac ggggtgacgg tgaacgcggt
     9241 ggcaccgggg tggatcgaga ccggctccca gacgccggac gaggcggagg aggggcggca
     9301 taccccggtc ggtcgctcgg ggacagcggg cgaggtcgcc tccgccatcg cgtggctctg
     9361 cacgcccggt gccgcctatg tcaccggcca ggtgatcgtc gtcgacggcg gcaatgccat
     9421 cgccgaggag cggctcgtcg cgacgcccta gagaccgagg tgcaggcgca gggctgcgtc
     9481 gagttccgtc tgcgggattc ttggcagcct gccgaccgtg tggccgatcc gttcgacgga
     9541 gaccgagcgg acctgttcgg cctgggcctt cgaatcccgg ggaagacccg tctcggcagc
     9601 gggagggaat acctggaaag gggtggacgc gcgagatgcc gctcgggacc ggcacgacgg
     9661 tcaccacgcc gcgtcccaat cgctcggctg cggcattggc ctggtcgttg ctgacgatca
     9721 cggccggccg tagcgtgtcc gcctcgctcc cgagagtggg gtcgagattg acgcggcgga
     9781 tctcaccgcg gcgcatcgtc gagacctccg gtagcgagcg gtgccgacga cctgctgcgt
     9841 cgctcagccg gccgcgaccg agaaggcctc ctcgagaatg tccatgcctt cgtggagcag
     9901 gtcatcgccg atggtcagcg gcggcaggaa gcggaagacg ttgccgaggg tgccgcaggt
     9961 caacgtgacc aacccgcgct cgtggcaggc cttgttgatc gcgccggtca gggcggccgc
    10021 gggctccttg ctctcgcggt cggtcaccag ctccatggcg atcatcgcgc cgcggccgcg
    10081 gacctcaccg acctgctcgt acttttcggc gatccggccc aaccgctcat agaggatggc
    10141 ctcgatggcc ttggcccggc cgcacaggtc gtcctcctcc atcgtctcga tcgacgccag
    10201 agcggcggcg caggcgacgg ggttgccgcc gtaggtgccg ccgagaccgc cggggtggat
    10261 ggtgtccatg atgtcggcgc ggccggtgat gcccgagagc ggcatcccgc cggcgatgcc
    10321 cttggccgtc acgacgatgt cgggcacgac ctgctcgtgg tcgacggcga accagtcgcc
    10381 gctgcggcag aagccggact gcacctcgtc ggcgatgaac aggatgccct tgtcgcgaca
    10441 ccactgcgcg agcgcaggca ggaagcccgg cggcgggacg atgaacccgc cctcgccctg
    10501 aatgggttcg atgatgacgg cggcggtgtt ctcctcgccg atctgggcgt ggatctggtc
    10561 ggtgatgtag ttgaaggcct cggcctcgca gtgctccggc ccgcccggcc agcgataggg
    10621 gtaggccatc ggcacgcgat agagctcgga ggcgaagggc ccgaacttgt gcttgtaggg
    10681 catgttcttg gccgtcatcg ccagggtgag gttggtgcgg ccgtggtagg cgtggtcgaa
    10741 ggcgacgatg ccgtccttgc cggtgaaatg ccgcgccacc ttgacggcgt tctcgacggc
    10801 ctccgcaccg gaattgaaga gggcagaacg cttttcgtga tcgcccggag tgcgcttgtc
    10861 gagttcctcg cagacctgga cgtactcctc atacggcgtg accatgaagc aggtgtgcgt
    10921 gaagtcggcg atctgcttgc tcactgcgtc gatgacgcgc tggttgccat tgccgacggt
    10981 cgtcacggca atgccggagc cgaagtcgat cagctggttg ccgtcgacgt cgcgcagtat
    11041 gccgcccgcc cccgcctcga catagatcgg caggccggtg ctcacgcccg tcgacaccga
    11101 tgccagcttc cgctgctgca actcctggga cttcggcccg gggatggcgg tggtcaggat
    11161 gcggcgctgc tcgatcatgc tgccgatact aggggtggtt ggcgcgcggt gttaggggta
    11221 tgccgcgcgg gcggcggggc tggtctcgcg ggcgcgcagc cagggcagga tcgctaccaa
    11281 tgtcacgagc ccggtcacca cgaacggcca gtggtagccg ccactgagat ccacgagcgc
    11341 gccggccgcg atgggtccga ggatgccgcc ggcgtcctgc gccatctgga gggctgcgag
    11401 ggccgggccg ccgctgcgtt cctggccgac gatgtcggcc gcggccgcct gctgggcggg
    11461 ggtcatcgtg ccggcgccga cgccgatgat caggcaaagg ccgacgagca gcgggatggt
    11521 cacggcgaag ccggtccaca gcgtggcgaa tccgctgacc gcgagccccc cgatgacgaa
    11581 cggcttccgg ccgcgcacat ccgagcgtcg tcccgcccag gcgagcccga gcatgttgcc
    11641 gacggcgaag acggccaggg cgaggccggc gacccacggc tggtcgctga cgaccgcggc
    11701 ggcgaagagg ggcaggatcg ccacgcgcac accgaaattc gcccacccgt tggcgaagaa
    11761 ggagcccaag gcggcgcggt atgccgggtc gggcaggcac tcgcgcatcg tcaccgcggg
    11821 ccgcgcggtg ccgtcggcga ccggtcgccc cgcgtctttg ggcagcatcg cccagacgag
    11881 cgcggccgcc agaaggatgc acacggcata cgccaggaac ggcacccgca ggccgagccc
    11941 gccgagcagg cccccgatcg ccgggcccgc gaccccgccg atgaggaagg ccgcgccgta
    12001 tgccgaggag acccgcccac ggatcgccgg cggcgcgagc cgggccaaaa gagcaacggc
    12061 ggagatggtg aacatcgtcg aaccgagccc gccgagcccg cggtatgcga gcagctgcca
    12121 atagttgtgc gcgaatgccg ttgcggcgga ggagatcgcg acgatgacga ggccgagcag
    12181 atagatgggc cgttcgccga agcgggcgac caggcgcccg ccgccggggg cgccgacgag
    12241 ccggaagaac gcgaacgcgg agacgacgat cgaggcggcc gtggcgccga cgtcgaagga
    12301 cgtggcgaat gccggcagca ccggagtgat caggccgaag ccgacggcga tgacgaaggc
    12361 ggcggcgatg aggacctgga tctcccgggg tatgcgggca cggggttccg gcgcgtcctg
    12421 cggggagtgg agggtcatgg cgggcaccaa gtatgccggg tcgcgcgtgt cggcatgaaa
    12481 agatcggggc gtgacgacac cttccgcgcc cggctggtat gacgacccgg aggcgcccga
    12541 ccagctgcgc tacttcgacg ggatcgtgtg gacgcggcat accaccccgc gctcgacgcg
    12601 cgccggtgcc ggcgcggcga ccccccaggc cggcgctccg gttgcgcccc agccgcagca
    12661 gcagccgacc gggacggccg acccctacgg ctggcgcggg gccggctcgc agcaggggca
    12721 gccgccgcag cagccggatc agtacgggca gcaaccgcag tggagtccgc cgcgacagga
    12781 tcaggcgccg cagtggcggc cgcctcagca gcagtggggg cagccgcaac agcagtgggg
    12841 gcaacaggca cccgggtggg gcccggcggc cggccaggtc ggcccggcaa cgcccgatgg
    12901 ggtgccgctg gccggctact ggcagcgggt gggcgcctac atcctcgacc tgctcatcgt
    12961 cggagccctg acggcgatct gcggcggcta ctggctgctg aaggcgatcc ggccgtggct
    13021 ggaccaggtc accgacgcca tcaactccag cacgaccgac ccgcagcggc tcgaggacct
    13081 ctccaacagc ctctcgacct atctgaccgg ccgcgacttc atgatcttca cggcgatcag
    13141 cctgcttgtc ggactgatct accacgtctt cttcctgacc cggaccggcg ccaccccggg
    13201 caagatggcg ttgcggatca gcgtccgcga gcgggagcgg ccggggccgc tgccggtgca
    13261 ggtggcgctg cgccgctacc tgctgacatt cgtcgtcaac gtcctcggct gggtgccggg
    13321 cctgctcagc gtgctggcgg ggctggtggg catcgcggac ctgctctggc ccgcgtggga
    13381 cccgaagcga caggcgctgc acgacaagat cgccggcacc aacgtggtgc gcggcaagca
    13441 gccccggacg ccggcgaact cctgacgacc cggttaccca ttggtggttg tggtgatcac
    13501 gacaacgacc gatgggtagc ccggtggcaa gccctgacgt cgaacagccg gggtcagcgc
    13561 gccggcagga ctcggcccac gcactcgccg aggccgatga cggcgccgtt cgcgccgggg
    13621 gcggtggcgc ggaggatgac ggtgtcgccg tcttcgagga aggtgcgggt gctgccggcg
    13681 tcgagcgtga tgggttcctg accggaccag ctcagctcga ggaagcagcc gcgggtctcc
    13741 ttctccgggc ccgagatcgt gcctgaaccg aagatgtcgc cggaacgaag agtggcgccg
    13801 ttgaccgtca tgtgggtgag catctgggcg ggggagtagt acatatcgcg gtactccggc
    13861 cgcgagacga ccgtgccgtt ccactcgacc tcgtagtgga tgtccaggcc gtatgccgcc
    13921 gcgcccgtca ggtagggcag cagcgccgga tcttggccgg ggagagccac tttcgcctct
    13981 gcgagggcgg ccgtgggggt gatccaggcg ccgatggagg tggcgaagga cttgccgagg
    14041 aagggaccga gggggacgta ctcccaggcc tggaggtcgc gggcggacca gtcgttgagg
    14101 atggtgacgc cgaagatgtg atcccaggcc tgttcgagcg ggacgggctc gccgagggtg
    14161 gtggcgccgc cgacgacgaa gccgagttcg gcctcgatgt cgaggcggac actgggaccg
    14221 aagacggggg catcggcctc cggcggcttg cgctggccgc agggccgcac gatgtcggtg
    14281 ccggtgacga cgatggttcc ggagcggccg tgatagccga tgggcagatg cttccagttg
    14341 ggtgtcagcg gggcctggtc aggccggaac atccgcccga cattggtggc gtggtgctcg
    14401 ctggcataga agtcgacgta gtcggccacg gtgatcggca gcaggagggc cacgccgtcc
    14461 agggggtgga ggttcgcgcc gacgcaatcg tcgtactcgg cgctggcgag cgtctcctgc
    14521 agccaggtgc gggccgaggc ccaggcttgc ggcccttggt cgagaaacgg gttgagcgag
    14581 ccgcactgcc ataccgcccc cgactccatc cccgcccgct cggcgagccc gccgatggcc
    14641 agggcgtggt cggcgacccg gacgacgacg cgcgggccgt cggagaggtc ggccacgccg
    14701 tacggcaggt tggccaggcc gaagggttcg tcggcgggca ggtcgcgcca ccagctcatg
    14761 agcggggtcc ttccaggacg tcgagggtga tcagttccgt gatcgggtcg gtgacggtgc
    14821 agcagccgta tgacgggaag tcgccgcgca ggcgcgccgc cgatacgggg ttcagcgccg
    14881 agaggccggc gatgagtggg ccggggtcgc gctgctccag ggtggcctgg atggccgaca
    14941 ccggccggcc ggcccgcgcc gccgcgaaag cggcgaggac gttgaggacg ccgtgcatgt
    15001 cctgggggtt gcgcccgggt gcctcgggat agctgccgcg gaccgcgtgg tgcaggccgc
    15061 cggtcagctt gagcggcacc tccatggcga tggcgccct
//
LOCUS       DAOPJG010000068        14848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4235_length_14848_cov_7.789157, whole genome shotgun sequence.
ACCESSION   DAOPJG010000068 DAOPJG010000000
VERSION     DAOPJG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 14848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14848
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4235_length_14848_cov_7.789157"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /gene="hemE"
                     /locus_tag="PKU75_08055"
     CDS             complement(<1..1043)
                     /gene="hemE"
                     /locus_tag="PKU75_08055"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HPF80866.1"
                     /translation="MCAGAGRAAYDSRVNPAESPLVRAARGLPVPHAPVWFMRQAGRS
                     LPEYRAVREGIAMLDSCRRPDLVAEITMQPVRRHGVDAAIFFSDIVVPVAAVGLEIDI
                     VPGVGPVVAEPVRTHADLDRLPELTPDAIPDISESVRLLVAELGETPLIGFAGAPFTL
                     ASYLVEGGPSRNHVKTKALMHGDPDLWNALCARLAQISTAFLAVQVEAGASVVQLFDS
                     WAGALSRADYERYVQPHSAHVLAAMGELGVPRIHFGVGTGELLASMGAAGADVVGVDY
                     RVALPDAIERLGDRYAVQGNIDPALLFAPWDALERETRRILDEGRSAKGHIVNLGHGV
                     LPETDPDVLTRLVG"
     gene            complement(1070..1903)
                     /locus_tag="PKU75_08060"
     CDS             complement(1070..1903)
                     /locus_tag="PKU75_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPF80867.1"
                     /translation="MFSLLALASSLVWGTSDFVAGVKAKTHPAAAVVGWSQAVALLAT
                     SIAVLFRSPHFAAGDWPLWAALAGASGSSALVCFYAALSTGTMGVVAPIASLGVLFPV
                     VLGVLEGEAPSALTWLGILVAIVGVLLASGPELDGRVSRRPVVLACAAGAGFGITLYA
                     IDRGSAHSVLNTMWGMRLTSAVMFLAAALWLRKAGGMRWADAPVLVFVGLADLTANIL
                     FGLASSKGMVSVASVLGSLYPVITAALAYAVLHERLRPVQIAGAAATVAGVVLIAAGS
                     A"
     gene            2023..2592
                     /locus_tag="PKU75_08065"
     CDS             2023..2592
                     /locus_tag="PKU75_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HPF80868.1"
                     /translation="MTSSAPEDFARALTLLHSAALRPEIRLTEVPAPTRIAPHAVALT
                     ADVLADPHGEEELASGRFVLLHDPACPEPWGGAWRAVTFARAELEPEFATDPMLGEVG
                     WAWLEEALTEHGLEFTCEAGTVTRVISEGFGAMAERPSTVEIEIRASWTPLDDEPGRH
                     LSAWCDLLCTVAGLPPLPEGVVALPGRRR"
     gene            2589..3809
                     /locus_tag="PKU75_08070"
     CDS             2589..3809
                     /locus_tag="PKU75_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HPF80869.1"
                     /translation="MTDQLVPADLPAPGIPLDHPADGLPEVVESERGLDEAATAVAGG
                     SGPVSIDAERASGFRYGQRAYLVQIRREGAGTWLIDPIAVPDLSPLDRAIGTGEWILH
                     AATQDLACLAEVGLRPRQLFDTELAARLLGLPRVGLAAVVEHYLGLVLAKEHSAADWS
                     KRPLPTAWLRYAALDVEVLGEIRNLMGVDLARSGKSEWARQEFEALLDWAPTARQDPW
                     RRTSGLHRVKGQRGVAMVRELWFERDRIAAERDIAPGRIIADALLIEIAQHALGANPP
                     GSLPAGNRAIARYQRSWLTALDRARALRDADLPPVNLPSEGPPPPRAWADKNPLAADR
                     LARTRLALTAFAEEHSVPVENVCSPEPLRRVVWTPPPGAPATDAFDAGLAAYGVRPWQ
                     REIVAPMLHSAFTA"
     gene            3918..5129
                     /locus_tag="PKU75_08075"
     CDS             3918..5129
                     /locus_tag="PKU75_08075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPF80870.1"
                     /translation="MPRSLREVVFVDGVRTPFGKSGPKGIYFETRADDLVIKCIRELL
                     RRNPQLPPEKVEEVAIAATTQIGDQGLTIGRTAALLAGLPKTTPGYSIDRMCAGAMTA
                     VTSTAGQIAFGAIDIAIAGGVEHMGRHPMGEGVDPNPRIVAEQLVDPSALVMGKTAEN
                     LHDRYPQLTKERSDAYSVASQDKLAAAYADGKIQPDLVTVATRSAEKGYGLAVADEPP
                     RPGTTLEDLGALKTPFRSHGRVTAGNAAGINDGATACVLAAEETARELGLPIKMRLIE
                     FAFVGVEPEVMGIGPVPAAEKALAKAGLTIDDIGLFELNEAFAVQVIAFLDHFGIPDD
                     SPKVNLWGGAIATGHPLASSGVRLMNQLAAQFAEHPEVRYGMTAMCIGLGMGGAVIWE
                     NPHHADYGKDA"
     gene            5133..7226
                     /locus_tag="PKU75_08080"
     CDS             5133..7226
                     /locus_tag="PKU75_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPF80871.1"
                     /translation="MTATTEASATQTPALPDEVVTKALVRDVTLPKGAGTMALITLDN
                     GFDHTKPNTFGPATIAGLTAALDTLASRAGAGEIQAVGIVGKPFIFAVGADLTGVPYV
                     RERQTALDIARAGHAAFAKIMDLPVPTFAFVNGAAMGGGVEIALACDYRTISAGAPAL
                     ALPECFLGLVPGWGGCYLLPKLVGVQNALTVIIENPMNMNKMLKGPAAFKLGLADAMF
                     APADFLEESLGWAASVLTGETKVERPEVNKDEAAWNGAVDMSKAFVDVKTGGKSPAPY
                     RALDLIRAARTAERDAAFAAEDEALADLLMGDPLRAGLYSFDLVQKRAKRPAGAPDRA
                     LARPVTKIGVIGAGLMAAQMALLFAQRLEVPVLMTDLDQERVDKGIAYVQGQLAELQT
                     KGRITQDTANKLSALITGSTDKAGMADAEFVIEAVFEEISVKKKVFAEVEEIVSPECV
                     LASNTSSLSLTEMAADLKHPERVVGFHFFNPVAVMPLVEIIPGEKTDDASLATAFAVG
                     KGLKKTTILVKDSASFIVNRILGRFMGEYSKIVDEGTPSAIADQGVAGLAPMPPFVLL
                     GLVGPAIALHNSETLHRAFGDRFYVSPSLQKLVEAKGAAYDPAMQIQPDNPVELTADQ
                     VKERVLSAIAEEVGMMLDEGVAQAPQDIDLALITGAGFQFWNGGITPLLDREGISEKV
                     LGKRFLPPGAASVPA"
     gene            7420..8952
                     /locus_tag="PKU75_08085"
     CDS             7420..8952
                     /locus_tag="PKU75_08085"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPF80872.1"
                     /translation="MTDTYLSRIGNLIRDARRHKGMTQSDLAELLATSQSAIARIEQG
                     GQNLSLEMLARIGEALDSEFVSLGHSQPTHLRVEGGKQLSGTIEVKTSKNAAVALLCA
                     ALLNKGRTTLRSLARIEEVNRIVEVLGSIGVKTRWLPGTNDLEIIPPAKLDMDAIDID
                     AARRTRTVIMFLGPLLHELDHFQLPYAGGCDLGTRTVEPHMIALRDFGLNVHATQGYY
                     EAHVREGQSPAKPIVLIERGDTVTENVLLAAARNPGKTIVRNASPNYMVQDLCFFLQK
                     LGVKIEGIGSTTLTIEGVADIDVDVDYQPSEDPIEAMSLLAAAVVTDSAITIKRVPIE
                     FMEIELATLETMGMKFDLTPEYFSANGHTRLVDLTTIPGPLKAPADKIHPMPFPGLNI
                     DNLPFFALIAAKAEGTTMIHDWVYENRAIYLTELTKVGVQVQLLDPHRVMITGPTTKF
                     RAAEVGCPPALRPGVVVLLAMLAAPGTSVLRNVYVINRGYEDLAERLTALGAKVETFR
                     DI"
     gene            9130..11106
                     /locus_tag="PKU75_08090"
     CDS             9130..11106
                     /locus_tag="PKU75_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HPF80873.1"
                     /translation="MTVSDNRAALPMAGHTHGNRSPVTCHLRCADACFKPVPNQSTDE
                     TFHSIASRALSRRSAMTGAFVLSVAAATTLTPESAEAAGSNLPFNPIGATPDDVDAVR
                     VPGGYRWQPVIKWGDPLFSGVPALSARGTTPAAQARQFGYNCDYLDIMPDKGSTTRGI
                     LVANHEYTNEDIMFPPGTDARFMARTALAAHGMSVVEISRSGAGAAWKPVRDGRRNRR
                     ITGTTPFTMSGPAAGSPLLRTAADPKGRRVHGTLNNCAGGTTPWGTILSGEENWNQYF
                     LCAGTSEKEERYGLGPDGDERMWRSVDPRFDATRPGYRNEPNRFGWIVEVDPEDPAST
                     PVKHTALGRIKHEAASVAIDKATGKVVVYTGDDERFEYLYKFISRRHYDDSGTAAARE
                     HNKKLLNDGDLFVARFTGDGRQDGVCDGTGVWIPLTMGGKSMVPGMSLDEVLVYTRLA
                     ASKMKATPMDRPEDVQVSPMSGRVYMACTNNSKRGLPGEPGTDEANPRPENKDGHVVE
                     ITEMDGEHTSRRFTWLLFLICGDKDSAGTYFGGYTGPVSPIACPDNLAFDSRGNLWIS
                     TDGQPSAIGLDDALHCVETAGTYRGRVRQFLSVPAGAETCGPVIHDKDGSVFVAVQHP
                     GEDGSWEAPQSHFPDYGKGVFKGPRPAVIQVTRA"
     gene            complement(11224..11904)
                     /locus_tag="PKU75_08095"
     CDS             complement(11224..11904)
                     /locus_tag="PKU75_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPF80874.1"
                     /translation="MWYWFFKFIGLGPAAWFWLRLRWSGREHLPRSGPVILAANHVSF
                     LDPIGVSMGVPRKVVYVAKTKYYSGRSLGGRFLAWFLKAIGQLPIDPESATTANPAIE
                     TARRLLDDGGVVAIFPEGTRSPDGRLYRGRTGVARLALPSRVPVIPVGIVGSREALQA
                     LPRRSRGIRIIYGAPVDLSPWYDRADDPDAWREATDLLLARIAALTGQEYVDRYPTAD
                     EIGKRDSA"
     gene            complement(11910..13769)
                     /gene="dxs"
                     /locus_tag="PKU75_08100"
     CDS             complement(11910..13769)
                     /gene="dxs"
                     /locus_tag="PKU75_08100"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPF80875.1"
                     /translation="MSLLESITGPAAVKALTPEQLPQLAGEIRQFLIHSVSKTGGHLG
                     PNLGVVELTIAVHRVFDSPDDAIVFDTGHQSYVHKVLTGRQDFSGLRARGGLSGYPSR
                     SESEHDIVENSHASTSLSWAHGIARARRLQGETGRHTVAVIGDGALTGGMAWEALNNI
                     AADKQLPLILIVNDNERSYAPTIGGMADHLATLRTTQGYEHFLEWGKTTLRKTPRVGG
                     PVERALHGVKKGIKDIVAPQGLFEDLGLKYVGPIDGHDPVALEAALQRAKAFGGPVIV
                     HVLTKKGRGYEPARRDEADRFHAVGVINPETGLPFEIAGRSWTEEFADEMVDLGAERS
                     DVVGVTAAMTIPVGLGPFGTTYPERLIDVGIAEQHAVTMAAGLAFAGLHPVVAVYATF
                     LNRAFDQLLMDAALHKAGITLVLDRAGITGPDGASHHGMWDMTICGAIPGLRLNAPRD
                     GATVRRALREAVDVADAPTVIRFPKGTVGDPLEPVRTVDDLDIMADHTDGGETDLLLV
                     AVGSMVPTAITVAEKLAAEGVRCCVVDPRWVIPVSDSLVSLAASAQRVAVIEDNLVIG
                     GIGSQVETALRAAGHDVPVRCFGIPRGFHDHASRDQVLDLIGLTPEAIAAALT"
     gene            complement(13860..14030)
                     /locus_tag="PKU75_08105"
     CDS             complement(13860..14030)
                     /locus_tag="PKU75_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80876.1"
                     /translation="MTAHVFLSNATTLVAGDTNGKRDAFRKDLVTGAVVRVSVGTGTA
                     DDRVMRVPIRRR"
     gene            complement(14088..14468)
                     /locus_tag="PKU75_08110"
     CDS             complement(14088..14468)
                     /locus_tag="PKU75_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80877.1"
                     /translation="MFDSRPHAGPPGWPSAVPPPGVSGWEVPAVSWLLDHCPADYRLY
                     AGWRRHPVALAWLTERHVEAQLIAMRQAYREARVEMSDYVPPEGISELMNGLEREGVR
                     LLAAARGVKLIHEALQGKTFVAKL"
     gene            complement(14479..>14848)
                     /locus_tag="PKU75_08115"
     CDS             complement(14479..>14848)
                     /locus_tag="PKU75_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPF80878.1"
                     /translation="GRGADGVAIARGLVDCGAALGYRVPELYAGESVAGAPAPYPASC
                     RPQAWAAASAVALVSAVLGLVVDVPGRRIALSPMRPAPFGPVTVTGLRFGATRFTVEV
                     DATGNVGVSGLPVDVEVDIR"
BASE COUNT         2391 a         5308 c         5037 g         2112 t
ORIGIN      
        1 ccgacgaggc gggtgagcac atccgggtcg gtctcgggca gcacgccgtg accgagattg
       61 acgatgtgcc ccttcgcgga gcggccctcg tcgaggatcc gtcgggtctc gcgctcgagc
      121 gcatcccagg gtgcgaacag cagggcaggg tcgatatttc cttgcaccgc ataccgatcg
      181 cccaggcgct cgatcgcgtc gggcagcgcc acgcgatagt cgacgccgac gacgtccgcg
      241 cccgccgccc ccatcgacgc cagcagttcg cccgtgccga ccccgaagtg gatccgcggc
      301 acgccgagct cacccatggc ggcgagcacg tgggccgagt gcggctggac gtagcgttcg
      361 tagtcggccc gtgacagcgc tccggcccag gagtcgaaga gctgcacgac actcgccccg
      421 gcctcgacct gcacggcgag gaaggccgtc gagatctgag cgagcctggc gcacagggca
      481 ttccacagat ccgggtcacc gtgcatgagg gctttggtct tgacgtggtt gcggctcgga
      541 cccccctcga ccagatagct ggccagcgtg aacggtgcgc cggcgaaacc gatgaggggc
      601 gtctccccca actcggcgac cagcaaccgg accgattcgc tgatgtccgg gatggcgtcc
      661 ggagtcagtt ccggcagccg gtccaggtcg gcgtgggtgc ggaccggctc ggcgaccaca
      721 ggcccgactc cggggacgat gtcgatctcc aaccccaccg cagcgaccgg gacgacgatg
      781 tcggagaaga agatcgccgc atccaccccg tgccggcgca ccggctgcat cgtgatctcg
      841 gcgaccagat ccggccggcg gcagctgtcg agcatcgcga tgccctcgcg gacggcgcgg
      901 tactccggca acgaccggcc cgcctggcgc atgaaccaca ccggggcgtg cggcacgggc
      961 agcccgcggg cagcacggac gaggggcgat tcggcggggt tcacccgcga atcgtatgcg
     1021 gctcggcccg cgcccgcgca cagcttgggt ggtcgcgaac ccggcccgct caggccgagc
     1081 cagcggcgat gagcacgacg ccggcgacgg tcgcggcggc gccggcgatc tgtaccggcc
     1141 ggaggcgctc gtgcagcacg gcatacgcga gagcggcggt gatcacggga tagagcgaac
     1201 cgagcacgct ggcgacgctg accatgccct tcgaggaggc gaggccgaag aggatgttgg
     1261 ccgtcaggtc ggcgaggccg acgaacacca gcaccggggc gtcggcccac cgcataccac
     1321 cggcctttcg caaccacagc gcggcggcga ggaacatcac ggcggaggtc aaccgcatac
     1381 cccacatcgt gttgagcacc gagtgggcgc tgccgcggtc gatggcgtac aacgtgatgc
     1441 cgaagccggc cccggcggcg caggccagca cgaccgggcg ccgcgagacc cggccgtcca
     1501 gctccgggcc ggaggccagg agcactccca cgatcgcgac caggatgccc agccacgtca
     1561 gcgcactcgg cgcctcgcct tcgaggacgc ccaggaccac cgggaacagc accccgagcg
     1621 aggcgatcgg tgcgacgacc cccatcgtcc cggtgctcag cgcggcatag aagcacacca
     1681 gggccgagga ccccgaggcc ccggcgagcg cggcccacag cggccagtcg ccggcggcga
     1741 aatgcgggct gcggaacagc acggcgatgc tcgtcgcgag cagggcgacc gcttgcgacc
     1801 acccgacgac cgcggccgcc ggatgcgtct tggccttgac cccggcgacg aaatccgacg
     1861 tgccccacac gagtgacgac gcgagcgcga ggaggctgaa cacagccgca ccctaaccac
     1921 cgcagtgtgt ccggcgctcg gacacgacac ccgggcccgg tgcggcaata ccgcacttcc
     1981 ggcgtggcct gcgcctacgc tgcagtcgtg gccactcgca gcatgacgtc atcggcgccg
     2041 gaggattttg cccgggcgct cacgctgctg cactcggccg ccctgcgtcc ggagatccgc
     2101 ctgaccgagg tcccggcgcc aacccgcatc gctccgcacg ccgtcgcgct gacggccgac
     2161 gtcctcgccg acccccacgg ggaggaggag ctggcgtccg gccggttcgt cctcctgcac
     2221 gacccggcgt gccccgaacc ctggggcggg gcctggcgcg ctgtcacctt cgcgcgggcg
     2281 gagttggagc ccgagttcgc caccgacccg atgctcggcg aggtgggatg ggcgtggctg
     2341 gaggaggcgc tgaccgagca tggcctggag ttcacctgtg aggccggcac ggtgacccgg
     2401 gtgatctccg agggcttcgg tgcgatggcg gagcggccct cgaccgtcga gatcgagatc
     2461 cgcgcctcgt ggacccctct cgacgacgaa cccggccggc acctgagcgc gtggtgcgac
     2521 ctgctgtgca ccgtcgccgg actgcccccg cttcctgagg gggtcgtcgc gcttccgggg
     2581 cggcgccgat gaccgaccaa ctcgtgccgg ccgatctgcc ggctcccggc ataccgctcg
     2641 atcatcccgc cgacgggttg cccgaggtgg tggagagcga gcgggggctg gatgaggccg
     2701 cgacggccgt ggccggcggg tccgggcccg tctcgatcga cgccgaacgg gcgtcgggtt
     2761 tccggtatgg gcagcgggcc tacctggtgc agatccgccg cgagggcgcg ggcacctggc
     2821 tgatcgaccc catcgcggtg ccggacctca gtcccctcga ccgggcgatc ggcaccggcg
     2881 agtggatcct gcacgcggcg acgcaggatc tggcctgcct cgccgaggtc gggctgcggc
     2941 cgcggcagct tttcgacacc gaactcgcgg cccgcctgct cggactgccc cgcgtcggcc
     3001 tcgccgccgt cgtcgagcac tatctcgggc tggtgctggc caaggagcat tcggccgccg
     3061 actggtcgaa gcggccgctg ccgacggcct ggctgcggta tgccgcgctc gacgtcgagg
     3121 tgctcggcga gatccgcaac ctcatggggg tggacctggc gcgcagcggc aagtccgagt
     3181 gggcgcgcca ggagttcgag gccctgctcg actgggctcc caccgctcgc caggacccgt
     3241 ggcggcgcac ctccggcctg caccgggtca agggccagcg gggagtcgcg atggtgcgtg
     3301 agctgtggtt cgaacgcgac cggatcgccg ccgagcggga tatcgcgccc ggccgcatca
     3361 tcgccgatgc gctgctgatc gagatcgccc agcacgctct cggcgcgaac ccgcccggct
     3421 cgctgcccgc gggcaaccgg gccatcgccc gctatcagcg cagctggctg acggctctcg
     3481 accgggcccg cgccctgcgc gacgccgatc tgcctccggt caacctgccc tccgaggggc
     3541 cgcccccgcc gcgggcctgg gcggacaaaa accccctcgc cgccgaccgg ctcgcccgca
     3601 cccgattggc gctgaccgcc ttcgcagaag aacactcggt ccccgtcgag aacgtctgct
     3661 cccccgaacc cctgcgccgg gtcgtctgga cgcccccgcc cggggccccg gcgaccgacg
     3721 ccttcgacgc ggggctcgcg gcatacggtg tgcggccctg gcagcgcgag atcgtggcgc
     3781 cgatgctgca tagcgccttc actgcgtgag cgttctcacc cacctgacca cggggaacta
     3841 agcgcacgct tagttagagt ggcgactaat ggggccggcc tacgtgaccg gcccctccca
     3901 acactcaagg aggcagtatg ccgcgcagtc tgcgtgaggt cgtcttcgtc gacggcgtcc
     3961 ggacgccgtt cggcaagtcg ggcccgaagg ggatctattt cgagacccgg gccgacgatc
     4021 tcgtcatcaa gtgcatccgc gagctgctgc gccgcaaccc gcagctgccg ccggagaagg
     4081 tcgaagaggt cgccatcgcc gcgaccacgc agatcggcga ccagggcctg acgatcgggc
     4141 gcaccgccgc gctgctcgcc gggctgccca agaccacgcc ggggtactcg atcgaccgga
     4201 tgtgtgccgg cgcgatgacc gcagtcacct cgaccgccgg gcagatcgcg ttcggcgcca
     4261 tcgacatcgc catcgccggc ggcgtcgaac acatgggccg ccacccgatg ggcgaaggcg
     4321 tcgaccccaa cccgcgcatc gtcgccgaac aactcgtcga cccctccgcg ctcgtcatgg
     4381 gcaagacggc ggagaacctg cacgaccgct acccacagct gaccaaggag cgctcggacg
     4441 cctattccgt tgcgtcccag gacaaattgg ccgccgccta tgctgacggc aagatccagc
     4501 ccgacctggt gaccgtggcc acgcgcagcg cggagaaggg atacggccta gcggtcgcgg
     4561 acgagccgcc ccgccccggc acgacgctgg aggacctcgg cgcgctgaag acgcccttcc
     4621 gctcgcacgg tcgggtcacg gcgggcaacg ccgcaggcat caacgacggc gccaccgcgt
     4681 gtgtgctcgc ggccgaggag acggcccgcg agttgggcct gccgatcaag atgcggctga
     4741 tcgagttcgc gttcgtcggg gtcgagcccg aggtcatggg catcggcccg gtgccggcgg
     4801 ccgagaaggc actggccaag gccggtctga cgatcgacga catcggactc ttcgaactca
     4861 acgaagcctt cgcggtgcag gtcatcgcct tcctcgacca cttcggcatc cccgacgact
     4921 cccccaaggt caacctgtgg ggcggcgcca tcgccaccgg tcacccgctc gcctcctccg
     4981 gcgtgcgcct gatgaaccag ctggccgcgc agttcgccga gcaccccgag gtccgctacg
     5041 gcatgaccgc gatgtgcatc ggcctcggca tgggcggtgc cgtcatctgg gagaaccccc
     5101 accacgccga ctacgggaag gacgcctgag ccatgactgc caccactgag gcctcggcca
     5161 cccagactcc cgccctgccc gacgaggtcg tcaccaaggc gctcgtgcgg gatgtcacct
     5221 tgcccaaggg tgccgggacg atggccctga tcaccctcga caacggcttc gaccacacca
     5281 agcccaacac cttcgggccg gcgacgatcg ccggcctcac cgcggccctc gacactctcg
     5341 cctcgcgggc gggcgccggt gagatccagg ccgtcggcat cgtcggcaag ccgttcatct
     5401 tcgccgtcgg cgccgacctg accggggtgc cgtatgtccg tgagcgccag accgctctcg
     5461 acatcgcccg cgccgggcac gccgcgttcg ccaagatcat ggacctgccg gtcccgacgt
     5521 tcgcgttcgt caacggcgcg gcgatgggcg gcggtgtcga gatcgcgttg gcgtgcgact
     5581 accggacgat ctcggccggg gcgccggcgc tggcgctgcc ggagtgcttc ctcgggctcg
     5641 tcccgggctg gggcggctgc tatctgctgc ccaaactggt cggcgtccag aacgccctga
     5701 ccgtgatcat cgagaacccg atgaacatga acaagatgct caagggcccg gccgcgttca
     5761 agctcggcct ggccgacgcg atgttcgccc cggcggactt cctcgaggag tcgttgggct
     5821 gggcggcgtc cgtgctcacg ggcgagacga aggtcgaacg tcccgaggtc aacaaggacg
     5881 aggccgcatg gaatggcgcc gtcgacatgt ccaaggcctt cgtcgacgtc aagaccggag
     5941 gcaagtcgcc ggctccgtat cgagcgctgg acctgatccg cgccgcccgg acggccgagc
     6001 gcgatgccgc cttcgccgcc gaggacgagg cgctggcgga cctgctcatg ggcgacccgt
     6061 tgcgggccgg cctctactcc ttcgacctgg tgcagaagcg ggccaagcgg cccgccgggg
     6121 cgccggaccg ggcactcgcc cggcccgtca ccaagatcgg cgtcatcggc gccggcctga
     6181 tggccgccca gatggcgctg ctgttcgcgc agcggctcga ggttccggtg ctgatgaccg
     6241 acctcgacca ggagcgcgtc gacaagggca tcgcctatgt ccagggccag ctcgccgaac
     6301 tgcagaccaa gggccggatc acgcaggaca cggccaacaa gttgtcggcc ctgatcaccg
     6361 ggtccaccga caaggccggg atggcggatg ccgagttcgt catcgaggcg gtcttcgagg
     6421 agatctcggt gaagaagaag gtcttcgccg aggtcgagga gatcgtctcg cccgagtgcg
     6481 tgctggcctc caacacctcc tcgctgtcgc tgaccgagat ggcggccgac ctgaagcacc
     6541 cggagcgggt cgtcggcttc cacttcttca acccggtcgc agtcatgccg ctcgtggaga
     6601 tcatccccgg tgagaagacc gacgatgcct ctctggcaac ggcattcgcc gtcggcaagg
     6661 gcttgaagaa gacgacgatc ctggtcaagg actcggcgag cttcatcgtc aaccggatcc
     6721 tcggccggtt catgggcgaa tactccaaga tcgtcgacga gggcaccccc tcggcgatcg
     6781 cggaccaggg cgtcgccggt ctcgcgccga tgccgccctt cgtcctgctc ggcctggtcg
     6841 gcccggcgat cgcgctgcac aacagcgaga ccctgcaccg tgccttcggc gaccgtttct
     6901 atgtctcccc gagcctgcag aagctcgtcg aggccaaggg cgcggcatac gacccggcga
     6961 tgcagatcca gcccgacaac cccgtcgagt tgaccgccga ccaggtcaag gaacgcgtcc
     7021 tgtcggcgat cgccgaggag gtcgggatga tgctcgacga gggcgtcgcc caggccccgc
     7081 aggacatcga cctggccctg atcaccggcg ccggcttcca gttctggaac ggcggcatca
     7141 ccccgctgct ggatcgcgaa ggcatctccg agaaggtgct cggcaaacgc ttcctgcccc
     7201 ccggcgcggc ctccgtcccc gcctgatcga tgcactgggc ggagccgcac ttttccgccc
     7261 agtgccactt catagcggtg cgcatcgcaa cctttgcgag gcgcaccgct atgtttgcgc
     7321 cctgggcgga aaagtgacct gcgcccaccg gctgtcacgg gtcccccagc ggcgcgtcga
     7381 ttcggttcgt atctcacatg tgagttacca tggtggcttg tgaccgacac gtatctgtcc
     7441 cgcatcggca acctcattcg cgacgcgcgc cgccacaaag gcatgacgca aagtgacctg
     7501 gccgagctgc tcgccacctc ccagagcgcc atcgcccgca tcgaacaggg cggccagaac
     7561 ctcagcctcg aaatgctcgc ccgcatcggc gaagcgctgg acagcgagtt cgtctccctc
     7621 ggccactccc agccgaccca cttgcgtgtc gagggcggca agcagctctc cggcaccatc
     7681 gaggtcaaga cctccaagaa tgccgccgtg gccctcctgt gcgccgcctt gctgaacaag
     7741 ggccgcacca ccctgcgcag cctcgcccgc atcgaggagg tcaaccggat cgtcgaggtc
     7801 ctcggctcga tcggggtcaa gacccgctgg ctgccgggca ccaacgacct cgagatcatc
     7861 ccgccggcca agctcgacat ggacgcgatc gacatcgacg cggcccgccg cacgcgcacg
     7921 gtcatcatgt tcctcggccc gctgctgcac gagctggacc acttccagct gccctacgcc
     7981 ggcggctgcg acctcggcac ccgaaccgtc gagccacaca tgatcgcgct gcgcgacttc
     8041 gggctgaatg tccatgcgac acaggggtat tacgaagcgc atgtccgtga aggccagagc
     8101 ccggccaagc cgatcgtcct catcgaacgc ggtgacaccg tcaccgagaa cgtgctgctg
     8161 gccgccgccc gcaaccccgg caagacgatc gtgcgcaatg cctcccccaa ctacatggtc
     8221 caggacctgt gcttcttcct ccagaagctc ggcgtcaaga tcgagggcat cgggtcgacc
     8281 acgctgacga tcgagggcgt cgccgacatc gacgtcgacg tcgactacca gccgtccgag
     8341 gacccgatcg aggcgatgag cctgctcgcg gccgccgtgg tcaccgactc cgccatcacg
     8401 atcaagcgcg tcccgatcga gttcatggag atcgagctgg ccacgctcga gacgatgggg
     8461 atgaagttcg acctgacgcc ggagtacttc tcggcgaacg gccatacccg cctcgtcgac
     8521 ctgacgacga tccccggccc gctcaaggcg ccggcggaca agatccaccc gatgcccttc
     8581 cccggcctga acatcgacaa cctgcccttc tttgcgctca tcgccgcgaa ggccgaaggc
     8641 acgacgatga tccacgactg ggtctacgag aaccgcgcca tttatctcac cgagctgacc
     8701 aaggtcggcg tgcaggtgca gttactcgac ccgcaccggg tgatgatcac cggcccgacg
     8761 acgaagttcc gggcggccga ggtgggctgc ccgccggcgc tgcggcccgg cgtcgtcgtg
     8821 ctgctcgcca tgctcgccgc cccgggcacc tcggtgctgc gcaacgtcta tgtcatcaac
     8881 cgcgggtatg aggatctggc cgagcgcctg accgcgctcg gggcgaaggt ggagaccttc
     8941 cgcgacatct gagagaggct ggtcccgtcc cgcaccgggc ggcgccggcc tgggcggaca
     9001 gcacctgatg gttacccaac gatcgcctgg tgtccatctg ccgttgaccg ggcccgctgg
     9061 cgtgtctcct taggttgtga gcgccatcgg tccgggaggg atcggtgagt tccccctcga
     9121 ggagtcgcca tgaccgtttc cgacaaccgc gcggcactgc cgatggccgg ccacacgcac
     9181 ggcaaccgca gcccggtgac gtgccatttg cgttgcgccg acgcgtgttt caagcccgtg
     9241 cccaatcaga gcaccgacga gaccttccac tccatcgcga gccgcgcgct ctcgcggcgc
     9301 tcggcgatga ccggtgcctt cgtgctgtcg gtcgccgccg ccacgacgct gacgccggag
     9361 agcgccgagg cagccggcag caacctgccg ttcaacccga tcggcgccac gcccgatgac
     9421 gtcgacgccg tccgcgtccc cggcggctac cgctggcagc cggtcatcaa gtggggggat
     9481 cccctcttct ccggcgttcc ggcgctcagc gcgcggggca cgacgccggc cgcccaggcc
     9541 cgccagttcg gctacaactg cgactacctc gacatcatgc ccgacaaggg cagcacgacc
     9601 cgcggcatcc tcgtggccaa ccatgaatac accaacgagg acatcatgtt cccgcccggc
     9661 accgacgcga ggttcatggc ccgcaccgcg ctggccgcgc acgggatgtc ggtcgtcgag
     9721 atctcccgct ccggcgccgg cgcagcctgg aagccggtgc gggacggccg ccgcaaccgc
     9781 cgcatcaccg gcaccacccc cttcaccatg tccgggcccg ccgccggctc cccgctgctg
     9841 cgcaccgcgg ccgaccccaa gggccggcgc gtccacggca ccctgaacaa ctgcgccggc
     9901 ggcaccaccc cgtggggcac catcctctcg ggcgaggaga actggaacca gtacttcctg
     9961 tgtgccggca cctccgagaa ggaggagcgt tatggcctcg gccccgacgg cgatgagcgg
    10021 atgtggcgca gcgtcgatcc acgcttcgac gccaccaggc cgggctaccg caacgagccg
    10081 aaccggttcg gctggatcgt cgaggtcgat ccggaggacc cggcgtcgac gccggtcaag
    10141 cacacggctc tggggcggat caagcacgag gccgcgagcg tggccatcga caaggcgacc
    10201 ggcaaggtcg tcgtgtacac cggcgacgac gagcgtttcg agtacctcta caagttcatc
    10261 tcccgccggc actacgacga ctccggcacg gccgcggccc gcgagcacaa caagaagctc
    10321 ctcaacgacg gcgacctgtt cgtcgcccgg ttcaccggcg acggccgcca ggacggcgta
    10381 tgcgacggca ccggcgtctg gatcccgctc acgatgggtg gcaagtccat ggtgccgggt
    10441 atgtcgctcg acgaggtgct cgtgtacacc cgcctcgccg cgtcgaagat gaaggccacg
    10501 ccgatggatc gtcccgagga cgtccaggtc agcccgatgt ccggccgggt ctacatggcc
    10561 tgcaccaaca actccaagcg cgggctgccg ggtgagcccg gcaccgacga ggccaacccg
    10621 cgtccggaga acaaggacgg tcacgtcgtc gagatcacgg agatggacgg tgaacacacc
    10681 agccgtcgct tcacctggct gctgttcttg atctgtggcg acaaggattc cgccggcacc
    10741 tacttcgggg gttacaccgg cccggtctcc ccgatcgcct gcccggacaa tctcgccttc
    10801 gacagccggg gcaacctgtg gatctccacc gatggccagc cgagcgccat cggtctggac
    10861 gacgccctgc actgcgtcga gaccgccggc acctatcgcg gccgcgtccg ccagttcctg
    10921 tccgtcccgg ccggcgccga gacctgtgga ccggtcatcc acgacaagga cggatcggtc
    10981 ttcgtcgcgg tgcagcaccc gggcgaggac ggatcgtggg aggccccgca gtcccacttc
    11041 cccgactacg gcaagggcgt cttcaagggc ccccgccccg ccgtcatcca ggtcacccgc
    11101 gcctgaaaac cactgcgctc caaggaactt ctgctagcag aggttagcaa aatcagcaca
    11161 cctagggagg tctgctgatt tgaccagcct ctgctagcag aggttcctcg ggggggccgg
    11221 aggtcaggcg ctgtcgcgct tgccgatctc gtcggcggtg ggatagcggt cgacatactc
    11281 ctggccggtg agcgcggcga tgcgggctag gaggaggtcg gtcgcctcgc gccaggcgtc
    11341 ggggtcgtcg gcgcggtcgt accacggcga aaggtcaact ggcgcaccat agatgatgcg
    11401 tatgccgcga gatcgcctgg ggagtgcctg cagcgcctcc cggctaccga cgatgcccac
    11461 cggtatgacg gggacccggc tcggcagggc cagtcgggcg acgccggtgc gcccgcggta
    11521 gaggcggccg tcgggtgatc gagttccctc gggaaagatc gcgacgacgc ccccgtcgtc
    11581 gaggaggcgc cgggcggtct cgatggccgg gttggcggtg gttgcgctct cggggtcgat
    11641 cggcagctgg ccgatcgcct tgaggaacca cgccaggaag cgtccgccga ggctgcggcc
    11701 cgagtagtac ttcgtcttgg cgacgtagac gaccttgcgc ggcactccca tgctcacccc
    11761 gatggggtcg aggaaggaga catggttcgc ggcgaggatc accgggccgc tgcggggcag
    11821 gtgctcgcgg ccggaccagc gcagccggag ccagaaccac gcggccggac cgaggccgat
    11881 gaacttgaag aaccagtacc acatggcgct caggttaagg ctgcggcgat ggcctcgggg
    11941 gtcaggccga tgaggtcgag cacctgatcg cgggaggcgt ggtcgtggaa gccgcgcggt
    12001 atgccgaagc agcgcaccgg cacatcgtgc cccgcagcgc gcaacgcagt ctcgacctgg
    12061 ctgccgatcc cgccgatgac gaggttgtcc tcgatgaccg cgacgcgctg cgccgaagca
    12121 gctagcgaca caagggaatc ggacaccggg atgacccagc gcggatcgac gacgcagcag
    12181 cgcacgccct cggcggcgag cttctccgca accgtgatgg ccgtcgggac catggagccg
    12241 accgcgacca ggagcaggtc ggtctcgccc ccgtcggtgt ggtcggccat gatgtcgaga
    12301 tcgtcgaccg tgcgcaccgg ctccagcgga tcgccgacgg tccccttcgg gaagcggatg
    12361 accgtggggg cgtcggcgac gtcgaccgcc tcgcgcaagg cccggcgcac ggtcgccccg
    12421 tcgcgcggcg cgttcagtcg caggccgggt atcgctccgc agatggtcat gtcccacatg
    12481 ccgtggtggg acgcgccgtc aggtccggtg atgccggcgc ggtcgagcac gagcgtgatg
    12541 ccggccttgt gcagcgccgc gtccatgagc agctgatcga aggcccggtt gaggaaggtc
    12601 gcatagacgg cgacgaccgg gtgcaggccg gcgaacgcca gcccggccgc catcgtgacg
    12661 gcatgctgct cggcgatgcc gacgtcgatg agccgctccg ggtacgtcgt gccgaacggg
    12721 cccagaccga ccgggatggt catcgccgcc gtcacgccga cgacatcgga gcgctcggca
    12781 ccgagatcga ccatctcgtc ggcgaactcc tcggtccagc tgcggccggc gatctcgaag
    12841 ggcagccccg tctcggggtt gatcacgccg acggcgtgga aacggtcggc ctcgtcgcgg
    12901 cgggccggct cgtagccgcg accctttttg gtcaggacat gcacgatgac cgggccgccg
    12961 aaagccttgg cgcgctgcag tgcggcctcg agggcgaccg ggtcgtgacc atcgatcggc
    13021 ccgacgtact tcaacccaag atcttcgaaa agaccttggg gcgcaacgat atccttgatg
    13081 cctttcttga cgccgtggag cgcgcgctcg accggcccgc ccacccgtgg ggtcttgcgc
    13141 agcgtcgtct tgccccactc gaggaagtgc tcgtagccct gggtcgtgcg cagcgtggcg
    13201 aggtggtcgg ccatcccgcc gatggtgggg gcataggagc gctcgttgtc gttgacgatc
    13261 aggatcagcg ggagttgctt gtcggcggcg atattgttca gcgcctccca cgccatgccg
    13321 ccggtgagtg ccccatcgcc gatgacggca accgtatgcc gtcccgtctc gccctgcagc
    13381 cgccgcgccc gcgcgatgcc gtgggcccag gacagcgacg tcgaggcgtg ggagttctcg
    13441 acgatgtcgt gttcggactc ggagcggctg ggatagccgg agaggccgcc ccgggcgcgc
    13501 aggccgctga agtcctgccg tccggtgagc accttgtgca cataggactg gtgaccggtg
    13561 tcgaagacga tggcgtcgtc ggggctgtcg aagacgcggt gcacggcgat cgtgagttcg
    13621 acgacgccga ggttggggcc gagatggccg ccggtcttgg aaaccgaatg gatgaggaac
    13681 tggcggatct cgccggccag ctggggcaac tgctccggcg tgagggcctt gacggcggcg
    13741 gggccggtga ttgactccag cagactcacg agtgcgcccg gtccttccag ggtggtatgc
    13801 cgcggggtcg agcggcgcgt aaacccttaa agtttacgcg ccggtcgcgg gcgcgccgct
    13861 catctgcgcc gaatcggcac gcgcatcaca cgatcgtcgg cggtcccggt ccccacgctg
    13921 acccgcacga cggcgccggt gacaaggtcc ttgcggaagg cgtcgcgctt gccgttggtg
    13981 tcgccggcga ccagggtggt cgcgttcgag aggaagacgt gtgcggtcac agagcagccc
    14041 gcccggctcg gtgagacacc cacggcggac gttgtcgaag ccggtcgtca cagcttcgcg
    14101 acgaaggtct tgccctgcag cgcctcgtgg atgagtttga cgccacgagc ggccgccagc
    14161 agccgcaccc cctcccgctc cagcccgttc atgagctcag aaattccttc tggcggaaca
    14221 taatcgctca tctcgacgcg tgcctctcga taggcctggc gcatggcgat cagctgggcc
    14281 tcgacgtgcc gctcggtcag ccaggcaagc gcgacgggat gacggcgcca cccggcatac
    14341 agccgatagt cggcgggaca gtggtcgagc agccacgaca ccgccggcac ctcccagccg
    14401 gacacgccgg gcgggggcac ggcgctcggc cacccgggag gtcccgcatg cggccgagaa
    14461 tcgaacacat gttctaggtt accggatgtc gacctcgacg tcgacgggca gcccggacac
    14521 tcccacattg cccgtggcgt cgacctcgac ggtgaagcgg gtcgcgccga agcgcagccc
    14581 ggtcacggtc accggaccga acggcgccgg ccgcatcggc gagagcgcga tccggcgacc
    14641 gggcacgtcg accaccagac cgaggacggc cgagacgaga gccactgccg aggccgcggc
    14701 ccacgcctgc ggccgacagg acgccggata cggcgccggt gccccggcga ccgattcgcc
    14761 ggcgtagagt tccggcaccc gatatccgag tgccgcgccg cagtcgacga gaccacgggc
    14821 gatagccacg ccatctgcgc cacgaccc
//
LOCUS       DAOPJG010000069        14755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4274_length_14755_cov_7.823537, whole genome shotgun sequence.
ACCESSION   DAOPJG010000069 DAOPJG010000000
VERSION     DAOPJG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 14755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14755
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4274_length_14755_cov_7.823537"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1779
                     /locus_tag="PKU75_08120"
     CDS             229..1779
                     /locus_tag="PKU75_08120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPF80879.1"
                     /translation="MTAKRLTPMDAMFLYGETPETMMHVGGLMTFTPAADAPADFLRQ
                     LMEEFKTTSPVYAPWNMKLKTPGFLYNPAHQWVDDPHFDIEYHVRRSALPHPGDEREL
                     GILVSRLHSNPLDLTRPPWEVHLIEGLERGRFAIYVKFHHALVDGYSAMKLLERSLSR
                     DPDTREEVLFFSLPPAKRREPADGKTHSWPVADVGAVVSRALTVTKSVPTLASSLLKL
                     QVRRDGDFKHLVSSFQAPHSVLNAKIGRNRRFATQQYEVDRLLAVGRAGGATLNDVVL
                     AICGGGLRRFLTESGQLGDAPLVAFLPVNVRPKGDVGGGNAVGAILSTLGTDIADPVQ
                     RLEVISRSTRSAKRNLTNLPMESILAYSAMLMAPAATQILSAVTGLPAPVPFTFNLCI
                     SNVPGPRSVLYFRGARMEADYPLSIPIHGMALNITCQSYAGTLDFGFIGDRDALPHLQ
                     RLAVYTGEELALLEDAFGLAVPDVKPKPAAKKAAAKKAPAKKAPAKKPATTKAPAKKA
                     AARKRPAS"
     gene            1827..3395
                     /locus_tag="PKU75_08125"
     CDS             1827..3395
                     /locus_tag="PKU75_08125"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPF80880.1"
                     /translation="MKPKVALSAAAAALGGVAIHDLTQKKHSLMRNFPVIAHARFALE
                     EIGPELRQYLITSNDEERPFSRDQRSWIYASSKLQNNYTGFGTDNDIENGEYVVFKQH
                     TFSDAVPPTAIHAGEHVWMPGPKVIGKARGRRDAFRPNSVVNISAMSFGSLSGNAIAA
                     LNGGAKLAGCLHNTGEGGLSQHHRQGGELVLQIGTAYFGCRDGEGRFDLDKLMKVVES
                     APVRAIEIKISQGAKPGLGGMLPAAKITEEIAGIRGIPMGKDCSSPSRHAEFSDVDGL
                     LDFVEKIAQATGKPVGIKSAVGDLIFWEQLVEQMRSGARGVDFVTIDGGEGGTGAAPL
                     VFTDAVSLPFRVGFSRVYKIFAQVGIQDRVVFIGAGKLGLPENALVAFALGADQVNIA
                     REAMMAIGCIQAQKCHTDHCPTGVATQSKWLAHGLDPELKSVRLANYIRTLRRDLLKV
                     SEACGVWHPAMLNADDVEIMDGNRRGTPLRELYGYEPTWGGLSGTIRDEIVAAMEATA
                     PQGGSAPPSPDAEL"
     gene            3574..4224
                     /locus_tag="PKU75_08130"
     CDS             3574..4224
                     /locus_tag="PKU75_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HPF80881.1"
                     /translation="MAVQVDDELSDVHTDGVQLPVPDRVRKILFGEMLIFAILSLIAS
                     WVLSYDAIVLARDPDAVLSCSINSVLDCAKVGTTWQANIFGFPNAFLGMISEPVVMTI
                     AVASLAGVRFPRWFMFVGNVCYLLGVIFAYWLLYQSTFVIGALCPWCLLVTLSTTFVF
                     SSMTQWNIRENNLFLPERWQRKALAFVENGWFTIALIAWVAAVIIFEVIKWRHRLF"
     gene            complement(4692..5759)
                     /gene="aroB"
                     /locus_tag="PKU75_08135"
     CDS             complement(4692..5759)
                     /gene="aroB"
                     /locus_tag="PKU75_08135"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPF80882.1"
                     /translation="MSELARVPVGTAYDVVLGRGVLAGVADLVPDSARRVLIVHSDTL
                     PQVVGPVRDALRAKGLHVVDGVVPDAEAAKTIDVAAGLWGLLGRESFTRTDVVVGVGG
                     GTVTDLAGFVAAAWLRGVPVVNVPTTVLAMVDAAVGGKTGVNTAEGKNLVGAFYEPLG
                     VLCDIDVLATLPEADFAAGMAEVIKAGFIADPVILELAEAHPGSLRRVGPATVELIER
                     AIAMKASVVTQDLREAGLREILNYGHTLGHAIEQHTAYTMRHGEAVAIGMVFAAELAH
                     RSGLIDDALLARHRSALALAGLPTAYDGAGLDELLAAMGRDKKTRGSTLRFVVLEGLA
                     RPTRFVGPSEGLLRAAYDALA"
     gene            complement(5756..6325)
                     /locus_tag="PKU75_08140"
     CDS             complement(5756..6325)
                     /locus_tag="PKU75_08140"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPF80883.1"
                     /translation="MVKPLVILIGPPGSGKTSVGARVAERLDVALEDTDARIEANEGR
                     TIAEIFIDSGEPYFRDVERAVVLHAVGAHGGVLALGGGSPMDPEIQAALTDRRVVFLD
                     VGIADAAKRVGFDASRPLLAVNPRARWVALMKERRGTYEALATHRVDTAGRSADEVAA
                     EIVELLIAEGVVPAAVESAVPHPAPGESS"
     gene            complement(6319..7545)
                     /gene="aroC"
                     /locus_tag="PKU75_08145"
     CDS             complement(6319..7545)
                     /gene="aroC"
                     /locus_tag="PKU75_08145"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPF80884.1"
                     /translation="MLRWLTAGESHGQALVATIEGLPSGVEVTTADIGAALARRRLGF
                     GRGARMAFEQDEVTFLGGVRHGHTLGSPVAIMIGNSEWPKWQTVMSADPVSEEAFAAS
                     DDVNAPQEIGRNRPLTRPRPGHADLVGMQKYAFEDARPILERASARETAARVALGEVA
                     ARLLAQAYGIRLVSHTISIGTASVGTDAELPHPDQVDAIDADPVRTLDAAGSAAMIAE
                     VEAAKKDGDTLGGVVEVVAYDVPPGLGSHVHWDRRLDARLAGALMSIQAIKGVEVGDG
                     FETARRRGSAAHDEMERDEAGVIRRRTGRAGGTEGGMSSGGLLRVRAAMKPISTVPRS
                     LDTVDIADPDAEAKAIHQRSDVCAVPAAGVVAEAMVALVLAQACLEKFGGDSLGETRR
                     NFESYLASIPEGMRTW"
     gene            7885..10062
                     /locus_tag="PKU75_08150"
     CDS             7885..10062
                     /locus_tag="PKU75_08150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPF80885.1"
                     /translation="MLARAVREVEAAARRGGRVSPAARAKFQVAALLVREERARVKSD
                     PEITPAEQANALRRLDGLATILAKTSARDTSLLTLLADDAVLSSAARDLRRKMLIAGG
                     YDVPDEAPAPPPPSADSAPAERQVVPQSVTQRHLANPFLAPDFSAAQELRRPRARVLA
                     GWELISPLLRAFEQSTGSAPACMPLPEPSRRGVPAGLTLMKHQAEVVAAAEQGHRTFL
                     LADEPGLGKTAQALLAARAASAYPLLVVVPNVVKTNWAREAQTWTPERRVTVVHGDGD
                     TVDGFADIVVVNYEVLERHVGWLGRHDFRGMVVDEAHFIKNEKSKRSRHVLQLSDKIR
                     SRMGRPLLMALTGTPLINDIEDFTAIWKFLGWIDDKGPRAALMNRLEDAGLTPADRGF
                     YAAARQAVIDMGIVRRRKVDVAADIPARRIADLPVELDDDAGRTIREAEEELAAHLVR
                     RYHRAVEVRMQQRPGDPPLEGIDLDLVRRVATAVREETSETAAGDNVFSLLRRIGQAK
                     ARLSADFAAQLAHNVGKVVYFAKHLDVMDVAEEVFARQGLRFATIRGNQTRTVRQANI
                     DAFVEDPEVAVIVCSLSAAGVGVNLQVASNVVLAELSWTDAEQTQAIDRVHRIGQDMP
                     VTAWRIIAAQTLDTKIAELIDSKAGLAARALDGSDEDFSSVDVQVEALVRLLTDALRA
                     ESGEAESLEASESSAGYAAAPRARTARSAPTAIAFASAPEARD"
     gene            complement(10077..10907)
                     /locus_tag="PKU75_08155"
     CDS             complement(10077..10907)
                     /locus_tag="PKU75_08155"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001311.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPF80886.1"
                     /translation="MPQAYVLGSPIGHSLSPAMHGAAYVALGLTDWSYAAHTVDESSF
                     AQFVAGCGPEVRGLSLTMPLKEIAFTVAEPVSHRAELAGAINTLVRTESGWLGDNTDI
                     LGVIGALAPEGRVARGRHSGLLLGAGATGRSAVIALRELGCRTVTACARNPGKALDSL
                     GPLTEKLDVELTVRELEAWPTVKSDLAVSTLPPSGARAATDVLEAGEADLSGIRLLDV
                     VYADWPTPLARAAMARGASVVSGLDMLAHQAVEQVELMTGLRPDWKVLLEAGQAACTG
                     "
     gene            complement(10921..12066)
                     /gene="mltG"
                     /locus_tag="PKU75_08160"
     CDS             complement(10921..12066)
                     /gene="mltG"
                     /locus_tag="PKU75_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPF80887.1"
                     /translation="MTQRLDQEIFGGANPTTGEPPGRPPRPRFFSNLAALLLSLAVVL
                     GGAYGLYRVAAPAISDFVATDDYPGPGSGEVEVVIKEGDSGRAIAATLVRAGVVKTSS
                     AFVQASSDDADIAEKLQPGTYTLLKEMKASDALTMLADPKNRSVKRVTIPEGLWASEI
                     YERLSKGTKVPLKDYVAAAKDTKTLGLPAAAKGNVEGWLFPETYEFGPKDTAAQQLKV
                     LVDQAKKVMTSEKIPESKWQETMIMASIIESEAGSSSDRRKVSRVFWNRLEQPTAETV
                     GLLQSDATVSYGAKRRAVAPTQAELDDSSNPYNTRIHKGMPPGPISNPGKDSIDAAVN
                     PAKGDWLYFVTVNPDTGETKFSETHEQFLKDSEEFRQWCADNPKAKC"
     gene            complement(12070..12612)
                     /gene="ruvX"
                     /locus_tag="PKU75_08165"
     CDS             complement(12070..12612)
                     /gene="ruvX"
                     /locus_tag="PKU75_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPF80888.1"
                     /translation="MSLRRGVRLGVDVGTVRVGVAACDPDGVLAYPVSTLARAGELGR
                     ADGIPPAQESEEAPADIAELARLVREMAVIEVVVGLPRSLDGGEGPAAALARGYSRLI
                     AAAVDPVPVRLIDERMTTIDAHRNLRSSGVAGRAQRRVVDQAAAVLILQAALDAERAT
                     GAPPGTPATTRRRKPRRKGT"
     gene            complement(12609..>14755)
                     /gene="alaS"
                     /locus_tag="PKU75_08170"
     CDS             complement(12609..>14755)
                     /gene="alaS"
                     /locus_tag="PKU75_08170"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPF80889.1"
                     /translation="VDEDRFMEFWNLVFMQYELSAVRSKEDFDVAAELPNKNIDTGMG
                     LERIASILQGVDNMYEIDEVKPVLVRAAEMTGKRYGVHSGHSAGESHPDDVRLRVVAD
                     HVRSALMLIGDGVTPGNEGRGYVLRRMLRRAVRSMRLLGYNEPCLPELLPISMDKMKA
                     SYPELESGFGRISQIAYAEEEAFRRTLAAGTTILDVAVEKTKSAGGAVLSGDQAFALH
                     DTYGFPIDLTLEMAAEQGLQVDRDGFTRLMSEQRQRAKADAKAKKSGHGDTTAYRSVA
                     DALGHEVEFTGYQDVTGEATVAGLLIDGVSAASATSGQEVEIVLDRTPFYAEGGGQLA
                     DGGLLRLANGALVEVDDVQKPITGLIVHRGRVVDGEVGTGEQAFAEVDIARRRSISRA
                     HTATHMVHKAIREALGDTATQAGSENSPGRFRFDFNASSAVPASVLRDVEARVNQVLL
                     DDLAVRADVMTQAQARESGAMALFGEKYGDAVRVISVGDWARELCGGTHAERTSQLGL
                     VTLLGESSIGSGVRRVEALVGTDAYSFLARERAIVGQLTELMKGRPEELPERVHGLMG
                     KLKDAERELDKLRKESLAAAGAGLTASARDVGGVTFLGHDAGSDAAADDVRTMALDLR
                     GRLGNDRPAVVAVTGSGGGRPVIVVATNEAARGAGHKAGALVRVAAGVLGGGGGGKDD
                     IAQGGGQDPSRTGDALAAVEASLGSAATGSAR"
BASE COUNT         2228 a         5518 c         4827 g         2182 t
ORIGIN      
        1 gacgaccacc gcgagagcga gcagcaccgg caaccagagc caccgccaga ccccggcgcc
       61 aggtttcggc cgcggacgcc aggtgaccgg cgccgcgaca gtctccgcgc ccgctggctc
      121 actcgatgct cgctctgacg tggccgcagg ctaacccgat ggcgatcgga ccacgacagt
      181 tgcgcggggg cactcccgcg atcgcacggc atacggaaca atcccgccat gacagcgaag
      241 cggctgacgc ccatggacgc gatgttcctc tatggggaga cccccgagac gatgatgcat
      301 gtcggcgggt tgatgacctt cacaccggcc gccgacgcgc ccgccgactt cctccgccag
      361 ctgatggagg agttcaagac gacctcgccg gtctatgcgc cgtggaatat gaaactcaag
      421 acccccggct tcctctacaa cccggcacac cagtgggtcg acgacccgca cttcgacatc
      481 gagtaccacg tgcgccgctc cgccctcccc caccccggcg acgagcggga gctgggaatc
      541 cttgtctccc ggctgcattc gaacccactc gacctgaccc gcccgccgtg ggaggtgcac
      601 ctgatcgagg gcctcgaacg cggacggttc gcgatctatg tgaaattcca ccacgcgctg
      661 gtcgacggct acagcgccat gaagctcctc gaacgcagcc tctcccgcga ccccgacacg
      721 cgcgaggagg tgctcttctt cagcctcccc cccgccaagc gccgcgagcc cgcggacggc
      781 aagacccact cctggccggt ggccgatgtc ggggccgtcg tcagccgcgc actgaccgtg
      841 accaaatccg tgcccaccct cgcctcctcc ctcctgaaac tgcaggtccg ccgcgacggc
      901 gacttcaagc acctggtctc ctccttccag gcgccgcatt cggtgctcaa cgccaagatc
      961 gggcgcaacc gccgcttcgc gacgcagcag tatgaggtgg atcgcctcct cgccgtcggt
     1021 cgcgcggggg gagccaccct caacgacgtg gtgctggcca tctgcggcgg cggtcttcgc
     1081 cgattcctca ccgagagtgg gcaactcggc gacgcaccgc tcgtcgcctt cctgcccgtg
     1141 aacgtccgcc cgaagggcga tgtcggcggc ggcaatgcgg tgggcgccat cttgtcgacc
     1201 ctcggcacgg acatcgccga ccccgtgcag cggctcgagg tcatcagccg ttccacccgc
     1261 agcgccaagc ggaacctgac gaatctgccg atggagtcga tcctcgccta tagcgccatg
     1321 ctcatggcgc ccgccgcaac gcagatcctc tcggcggtga ccgggctccc ggcgccggtg
     1381 cccttcacct tcaacctctg catctccaat gtgcccggcc cgcgatcggt gctctacttc
     1441 cgcggcgccc ggatggaggc cgactacccg ctgtcgatcc cgatccacgg catggcgctg
     1501 aatatcacct gccagtcgta tgccgggacc ctcgacttcg gattcatcgg cgaccgggac
     1561 gccctgccgc acctgcaacg actggccgtc tacaccggcg aggagcttgc cctcctcgag
     1621 gacgccttcg gcctcgccgt tccggacgtg aagccgaagc ccgcggccaa gaaggccgcg
     1681 gcgaagaagg ctccagcgaa gaaggccccg gccaagaagc ctgcgaccac gaaggccccg
     1741 gccaagaagg ccgccgcacg aaaacgaccg gcgagctgac cgcatacctg cccccatgac
     1801 gcgccccggc gcggcatcat gggcccatga agcccaaagt cgcgctttcg gccgctgcgg
     1861 ccgccctcgg cggcgtcgcg atccatgacc tgacgcagaa gaagcacagc ctgatgcgca
     1921 acttcccggt gatcgcgcac gcgcgcttcg cgctggagga gatcgggccg gagctgcggc
     1981 agtacttgat cacgagcaat gacgaggaac ggcccttcag ccgcgaccag cggtcctgga
     2041 tctatgcgtc ctcgaagctg cagaacaact acaccggctt cggcaccgac aatgacatcg
     2101 agaacggcga atacgtcgtc ttcaaacagc acaccttctc cgatgcggtg ccccccaccg
     2161 cgattcacgc cggcgagcac gtgtggatgc ccgggccgaa ggtgataggc aaggcccgcg
     2221 gccggcgcga cgccttccgc cccaactccg tggtcaacat ctcggcgatg agcttcggct
     2281 ccctgtcggg caacgccatc gccgccctca acggtggggc caagctcgcc ggctgcctgc
     2341 acaacaccgg cgagggcggc ctgtcccagc atcaccggca aggcggggaa ctcgtgctgc
     2401 agatcggcac ggcatacttc ggctgccgcg acggcgaagg acggttcgac ctcgacaagc
     2461 tgatgaaggt cgtcgagtcg gccccggtgc gggccatcga gatcaaaatc agccaaggcg
     2521 ccaaacccgg cctcggcggc atgctgcccg ccgccaagat caccgaggag atcgccggca
     2581 tccgcggcat acccatgggc aaggactgct ccagcccgtc gcggcacgcg gagttctccg
     2641 acgtcgacgg cctgctcgac ttcgtcgaga agatcgccca ggccaccggc aaaccggtcg
     2701 gcatcaagtc ggcggtcggc gacctgatct tctgggagca actcgtcgag cagatgcgat
     2761 ccggggctcg cggggtcgat ttcgtcacca tcgacggcgg ggagggcggc accggcgcgg
     2821 ccccgctggt cttcaccgac gccgtctcgc tgcccttccg ggtcggcttc tcgcgggtct
     2881 acaagatctt cgcccaggtg gggatccagg accgggtcgt cttcatcggc gccggcaagc
     2941 tcggcctgcc ggagaatgcg ctcgtcgcct tcgcgctcgg cgcggaccag gtcaatattg
     3001 cccgggaggc gatgatggcg atcggctgta tccaggcgca gaagtgccac accgaccact
     3061 gccccaccgg cgtggccacc cagagcaagt ggctggcgca cgggctggac ccggagttga
     3121 agtcggtgcg gctggccaac tacatccgca ccctgcgccg cgacctgctc aaggtctccg
     3181 aagcgtgcgg ggtctggcat ccggcgatgc tcaatgccga cgacgtggag atcatggacg
     3241 gcaaccggcg ggggacgccg ctgcgtgagc tctacggcta cgaacctacc tggggcgggc
     3301 tgtccggaac gatccgggac gagatcgtcg ccgcgatgga ggcgaccgcg ccgcagggcg
     3361 ggtcggcgcc gccctccccc gacgccgaac tctgagcgcg cctccgcctg agtcgaaagc
     3421 gcacgacacg ccgctatggc gcggtcatta cggcgcgtcg tgcactctcg aacgggggtg
     3481 gggggcgtgg gggcgtgggg gccgtgcgtt tcggggagta caaggtcgct gggttaatgt
     3541 gtggcgacgc gcgagcgacc tggaggacag cgcatggcag tgcaggtgga cgatgagctg
     3601 agcgacgtcc ataccgacgg cgtccagctg cccgtccccg accgggtccg caagatcctc
     3661 ttcggcgaga tgctcatctt cgccatcctc agcctgatcg cgtcctgggt gctgtcatat
     3721 gacgccatcg tgctcgcccg cgaccccgac gccgtcctga gctgctcgat caactccgtc
     3781 ctcgactgcg ccaaggtcgg cacgacgtgg caagccaata tcttcggctt ccccaacgcc
     3841 ttcctcggca tgatctccga gccggtcgtc atgacgatcg ccgtcgcctc gctggcgggg
     3901 gtgcgcttcc cgcggtggtt catgttcgtc ggcaacgtct gctacctgct cggcgtgatc
     3961 ttcgcctact ggctgctcta tcagtcgacc ttcgtcatcg gcgccctgtg cccctggtgc
     4021 ctgctcgtga ccctgtcgac gaccttcgtc ttctcctcga tgacgcagtg gaatatccgc
     4081 gagaacaacc tcttcctccc cgagcgctgg cagcgcaagg ccctggcctt cgtcgagaac
     4141 ggctggttca cgatcgccct gatcgcctgg gtcgccgcgg tgatcatctt cgaggtcatc
     4201 aagtggcgcc accgactctt ctagacggtg atgccagtcc gcccggacac gtcaccggag
     4261 caacaacggg gtcatgaccc cgttgttgct ccggtgacgg tctcgcccgg ccggagaatg
     4321 gggcacccct gcctgcggca caggtgagtt ggcccaccgc cttgtctcgc acctgccgca
     4381 cacccgccgg aatcccgtgc ggacgttcca cgtcgcggac ccagcggtgt tccagggtgc
     4441 tctcggcgcc cacggcgtcc tccgcccctc ttgagcgcct ccgccagggt cacgatgggc
     4501 agctcgtcgc gcgtcggaag ggttggccgt tgcccgatcc acgcgtatga ctgccccatt
     4561 cgccgatcgt gtcgcgaaag ccccaacccg tcgacaccgt gcgcccaccg ctgtggacaa
     4621 ccgacgccag tgccgtcgac ggagcgatat cggggtcctg accccgccat agctccggtg
     4681 acggctgcgt gtcaggcgag agcgtcgtat gccgcgcgca gcagcccctc gctcggcccc
     4741 acaaaccggg tgggccgggc caggccctcc agcacgacga accgcagcgt cgagccgcgg
     4801 gtcttcttgt cgcgccccat cgccgcgagc agctcgtcca gtccggcgcc gtcgtatgcc
     4861 gtgggcaggc ccgccagcgc gagcgcgctg cggtgccggg ccagcagggc gtcgtcgatg
     4921 aggccggagc ggtgggccag ttcggcggcg aacaccatcc cgatcgccac cgcctccccg
     4981 tgccgcatcg tgtatgccgt gtgctgctcg atcgcgtgcc ccagggtgtg cccgtagttg
     5041 aggatctccc gcagcccggc ctcgcgcaga tcctgggtca cgaccgacgc cttcatggcg
     5101 atggcgcgct cgatcaactc gacggtcgcc ggcccgaccc gccgcaagga gcctgggtgc
     5161 gcctcggcca gctccaggat caccggatcc gcgatgaacc cggccttgat cacctcggcc
     5221 atgcccgccg caaaatccgc ctccggcagc gtggccagca cgtcgatatc gcagagcacc
     5281 ccgagcggct cgtagaaggc gccgacgagg ttcttgccct cagcggtgtt gacgcccgtc
     5341 ttgccgccga ccgccgcatc gaccatggcg agcaccgtcg tcgggacatt gacgacgggc
     5401 acgccccgca gccacgccgc cgccacgaac ccggcgagat cggtcaccgt ccccccgccg
     5461 accccgacga cgacatcggt ccgcgtgaag gactcgcgcc cgagcagccc ccacagaccg
     5521 gccgccacgt cgatggtctt ggccgcctcg gcatcgggca cgaccccgtc gacgacgtgc
     5581 aatcccttgg cgcgcaaggc atcccggacc ggcccgacaa cctgcggcag ggtgtcgctg
     5641 tgcacgatca ggacgcgccg ggcggagtcc ggcaccaggt ccgcgacgcc ggcgaggact
     5701 ccccgcccca gcacgacgtc gtatgccgtg cccaccggca cccgggccag ctcgctcacg
     5761 atgactcgcc tggggccggg tgcggcactg cgctctcgac ggctgccggg acgacgccct
     5821 ccgcgatcag cagctcaacg atctccgccg ccacctcgtc ggccgagcgg ccggcggtgt
     5881 ccacccggtg cgtcgccagc gcctcatacg tccctcggcg ctccttcatc agcgccaccc
     5941 accgggcgcg cgggttgacg gcgagcaacg gccggctcgc atcgaaaccc acccgcttgg
     6001 ccgcatcggc gatccccaca tcgaggaaga cgacccgccg atccgtcaac gccgcttgga
     6061 tttccgggtc catcggggag ccgcctccga gggccaatac gcccccgtga gcgcccaccg
     6121 catggagcac cacggcccgc tccacgtccc ggaaataggg ctcgccggag tcgatgaaga
     6181 tctccgcgat cgtgcgcccc tcgttcgcct cgatccgcgc gtcggtgtcc tccaacgcga
     6241 catccaggcg ctcggccacc cgcgccccga cactcgtctt gcccgacccg ggaggcccga
     6301 tgaggatcac caagggcttc accacgtgcg catcccctcc gggatcgacg cgagatagga
     6361 ctcgaagttg cgccgcgtct cccccagcga gtcgcccccg aacttctcca ggcacgcctg
     6421 cgccagcacc agcgccacca tcgcctcggc caccacgccg gccgccggca ccgcgcacac
     6481 atcggagcgc tggtggatcg ccttcgcctc cgcgtccggg tccgcgatat cgaccgtgtc
     6541 cagcgagcgc ggtaccgtcg agatcggctt catcgccgcg cggacccgca gcagcccgcc
     6601 actcgacata ccgccctccg tgccgcccgc gcggccggtg cgccgccgga tcactccggc
     6661 ctcgtcgcgc tccatctcgt cgtgcgccgc cgacccccgc cggcgcgcgg tctcgaagcc
     6721 gtcgccgacc tcgacgccct tgatcgcctg gatcgacatc agcgccccgg ccagccgggc
     6781 atccaggcgc cggtcccaat gcacatgcga gccgaggcct ggcggcacgt cataggcaac
     6841 cacctccacc accccgccga gagtgtcgcc atccttcttc gccgcctcga cctccgcgat
     6901 catcgccgcg gacccggcgg catccagggt ccgcaccggg tcggcgtcga tggcgtcgac
     6961 ctgatcggga tgcggcagct cggcgtcagt cccgaccgag gccgtaccta tcgaaatcgt
     7021 gtgggagaca aggcgaatcc cgtatgcctg cgccagcagc cgcgcggcca cctccccgag
     7081 cgcgacccgc gccgccgtct cgcgtgccga cgcccgctcc aggatcggcc gggcatcctc
     7141 gaaggcgtac ttctgcatcc cgaccaggtc cgcgtgcccc ggccgcggcc gagtcaacgg
     7201 ccggttgcgg ccgatctcct gcggcgcgtt cacgtcatcg gaggccgcga acgcctcctc
     7261 gctgaccggg tcggccgaca tcaccgtctg ccacttgggc cactcgctgt tgccgatcat
     7321 gatcgccacc ggagagccga gggtatgccc gtgccgcacc ccgccgagga aggtcacctc
     7381 gtcctgctca aaggccatcc gcgcgccccg gccgaacccg agccgccgcc gcgccagcgc
     7441 cgcgccgata tcggcggtcg tcacctcgac tcccgacggc agcccctcga tcgtggcgac
     7501 cagggcctgc ccgtgggact cacccgcagt cagccaacgc aacatgttcg gcatcctcgc
     7561 acgcgaactc gagggcatcg tcgaccagtc cgcgggacgg tccgcaggcc ggacgtcgcc
     7621 tgagtcgagt gagcccgaca cgccgaagat tcggccgaac aatgggcgtg tcgcgctcac
     7681 tcgactgggg gacgtgagcc ggtgatggca caatagggtc caccactcgg gggggcgggc
     7741 gctctgcgac gcccctgccg acggtccgca tgccggaccc cgcttctcct agcccaggag
     7801 tgcttcttgg ccacccgtgc ccagcgccct gccgtgcgaa cggcacgccg cccacgtcat
     7861 agcgacgagg ccggcctcat acccgtgctc gcgcgcgccg tccgcgaggt cgaggccgcc
     7921 gcccggcgcg ggggccgcgt ctccccggca gcccgcgcca agttccaggt tgccgccctg
     7981 ctggtgcgcg aggagcgcgc ccgggtcaag tccgacccgg agatcacccc cgccgagcag
     8041 gccaacgccc tgcgccgcct cgacggtctc gcgacgatcc tcgccaagac ctccgcccgc
     8101 gacacctccc tgctcaccct gctcgccgac gacgccgtgc tctcgtcggc cgctcgcgac
     8161 ttgcgccgca agatgctcat cgccggcggg tacgacgtcc cggacgaggc tcccgcgccg
     8221 ccgcccccga gcgcggactc ggcgcccgcc gagcgtcagg tcgtccccca gtcggtgacc
     8281 cagcggcacc tggcgaaccc gttcctcgcc cccgatttct ccgcggcgca ggagttgcgc
     8341 cggccgcgcg cccgcgtgct cgccggctgg gagctgatct cccctctgct gcgcgccttc
     8401 gaacagtcga ccggttcggc gccggcgtgt atgccgctgc ccgagccgtc ccggcggggc
     8461 gtcccggccg gtttgaccct gatgaagcat caggccgagg tggtggccgc cgccgagcag
     8521 gggcaccgga ccttcctgct cgccgacgag cccggcctgg gcaagacggc ccaggcgctg
     8581 ctcgccgccc gggcggcctc cgcatacccg ctgctggtcg tcgtccccaa tgtcgtcaag
     8641 accaactggg cccgggaggc gcagacgtgg acccccgagc ggcgggtcac cgtcgtccac
     8701 ggcgacgggg acacggtgga cgggttcgcc gacatcgtcg tcgtcaacta cgaggtgctc
     8761 gagcggcatg tcgggtggct gggcaggcac gacttccgcg ggatggtcgt cgacgaagcg
     8821 cacttcatca agaacgagaa gtccaagcgg tcccggcacg tgctgcagct gagcgacaag
     8881 atccgctctc ggatggggcg gccgctgctc atggcgctga ccgggacccc gctgatcaac
     8941 gacatcgagg acttcacggc catctggaaa ttcctcggct ggatcgacga caagggcccg
     9001 cgcgctgcct tgatgaaccg gctcgaggac gccgggctca ccccggccga ccgcggcttc
     9061 tatgcggcgg cccggcaggc ggtgatcgac atgggcatcg tgcggcgccg caaggtcgac
     9121 gtcgccgccg acattccggc ccgccgcatc gcggatctgc cggtggaact cgacgacgac
     9181 gccgggcgga cgatccgtga ggccgaggag gaactcgccg cccacctcgt gcggcgctac
     9241 caccgggccg tcgaggtgcg gatgcagcag cgtccgggtg acccgccgct cgaaggcatc
     9301 gacctcgacc tggtgcgccg cgtcgcgacc gcggtgcgtg aggagacgtc ggaaacggcg
     9361 gccggcgaca atgtgttcag cctgctgcgc cggatcggcc aggccaaggc gcggctgtcg
     9421 gcggacttcg ccgcgcagct ggcccacaac gtcggcaagg tcgtctactt cgccaagcac
     9481 ctcgacgtca tggatgtcgc cgaagaggtc ttcgcccgcc agggcctgcg gttcgccacg
     9541 atccgcggca atcagacccg gacggtgcgg caggccaata tcgacgcctt cgtcgaggac
     9601 cctgaggtgg ccgtcatcgt ctgctcgctg tcggcagcgg gcgtcggcgt caacctccag
     9661 gtcgcctcca acgtcgtgct cgccgagctc tcgtggaccg acgccgagca gacccaagcg
     9721 atcgaccggg tgcaccggat cggtcaggac atgccggtga ccgcgtggcg gatcattgcc
     9781 gctcagaccc tcgacaccaa gatcgccgaa ctcatcgaca gcaaggccgg gctcgccgcg
     9841 cgggccctcg acgggtccga cgaggacttc tcctcggtgg acgtccaggt cgaggccctc
     9901 gtgcgcctgc tcaccgacgc cctgcgcgcc gagtcgggag aggccgagtc gctcgaggcg
     9961 agcgagagca gcgcggggta tgccgcagcc ccgcgggcgc gcacggcacg cagcgcaccc
    10021 acggccattg ccttcgcctc cgcgcccgag gcgagggact aggcaagcgg gcccgctcac
    10081 ccggtgcagg cggcctggcc ggcctcgaga agcaccttcc agtcgggccg caggccggtc
    10141 atcagctcga cctgctcgac ggcctggtga gccagcatgt cgaggccgct gacgacgctg
    10201 gccccgcgcg ccatggcggc gcgggcgagg ggggtgggcc aatccgcata gacgacgtcg
    10261 agcagccgta tgccgctcag gtccgcttcg ccggcctcga gtacgtcggt cgccgcacga
    10321 gcgcccgatg gcggcagcgt ggagacggcg aggtcggact tgacggtggg ccacgcctcg
    10381 agttccctca ccgtcaactc gacatcgagc ttctcggtga gcgggccgag ggagtcgagg
    10441 gccttgccgg ggttgcgggc gcaggcggtg acggtgcggc agccgagttc gcgcagggcg
    10501 atcacggccg accggccggt ggcccccgca ccgaggagca gcccggagtg ccgcccgcgc
    10561 gccactcgtc cttcgggggc cagagcgccg atgaccccca ggatgtcggt gttgtctccc
    10621 aaccaccccg attccgtccg gacgagggtg ttgatcgcgc cggccagttc cgcccggtgc
    10681 gacaccggtt cggcgacggt gaaggcgatc tccttcaacg gcatcgtcag cgacaaccca
    10741 cgaacctccg gcccgcagcc ggccacgaac tgcgcgaacg aggactcgtc gacggtgtgc
    10801 gcggcatacg accaatcggt cagaccgagc gcgacataag cagccccgtg catggccggc
    10861 gagagcgagt gcccgatcgg cgacccgagg acgtaagcct gcggcatacc cgagatcctg
    10921 tcagcacttg gccttcgggt tgtcggcgca ccactgcctg aactcctcgg agtccttgag
    10981 gaactgctcg tgcgtctcgg agaacttcgt ctcgccggtg tccgggttga cggtcacgaa
    11041 gtagagccag tcacccttgg ccgggttgac cgcggcatcg atcgagtcct tgcccgggtt
    11101 ggagatcggg ccgggcggca tccccttgtg gatccgggtg ttgtacggat tcgacgagtc
    11161 gtcgagctcc gcctgggtag gtgcgaccgc gcgccgcttg gcgccgtagg agaccgtcgc
    11221 atcggattgc agcagcccca ccgtctcggc cgtgggctgc tcgaggcggt tccagaagac
    11281 ccggctcacc ttgcgccggt cggaggagga gccggcctcg gactcgatga tcgaggccat
    11341 gatcatcgtc tcctgccact tgctctcggg gatcttctcg ctggtcatga ccttcttggc
    11401 ctggtcgacc aggaccttca gctgctgggc ggcggtgtcc ttgggcccga actcataggt
    11461 ctcggggaac agccagccct cgacattgcc cttggcggcc gcgggcaggc cgagcgtctt
    11521 ggtgtccttg gccgcggcga cgtagtcctt caacggaacc ttcgtgccct tcgagagccg
    11581 ctcgtagatc tcgctcgccc acaggccctc aggaatggtc acccgcttga cggagcggtt
    11641 cttcgggtcg gccagcatcg tcagagcgtc ggaggccttc atctccttca ggagcgtgta
    11701 ggtgcccggc tgcagcttct ccgcgatgtc ggcatcgtcg ctggaggcct gcacgaacgc
    11761 gctcgacgtc ttcaccacgc cggcgcgcac gagagtggcg gcgatggccc ggcccgagtc
    11821 gccctctttg atgacgactt cgacctcacc cgagcccggc cctggatagt cgtcggtcgc
    11881 cacgaaatcg ctgatcgcgg gcgccgccac acgatagagg ccataggccc cgccgagcac
    11941 gacggccagg gagagcagaa gagccgcgag gttgctgaag aagcgcggcc gggggggccg
    12001 gcctggcggt tcaccggtcg tgggattggc gcccccgaag atctcctgat cgagtcgctg
    12061 cgtcaccgct caagtgccct tccgtcgcgg cttgcgccgc ctcgtcgttg ccggggtccc
    12121 ggggggtgcc ccggtcgccc gctccgcatc cagcgccgcc tgcaggatga gaaccgcggc
    12181 agcctggtcg acgacccgcc gctgtgcacg tcctgccacc ccgctggaac ggaggttgcg
    12241 gtgcgcgtcg atcgtggtca tccgctcgtc gatcagacga accgggaccg ggtcgaccgc
    12301 tgccgcgatc agtcgagaat aaccgcgcgc gagcgctgcg gcgggaccct cgccgccgtc
    12361 gagcgaacgc ggcagcccga cgaccacctc gatcaccgcc atctcgcgca ccaatcgtgc
    12421 cagctcggcg atatccgccg gggcctcttc ggactcctga gcagggggta tgccgtccgc
    12481 ccggccgagt tcgccggcgc gggccagcgt cgacaccggg taggcgagaa cgccgtccgg
    12541 gtcgcacgcc gccaccccga cacgcacggt gccgacgtcg accccgaggc ggactccgcg
    12601 acgcaggctc agcgtgcact cccggtcgcg gctgacccga gagaggcctc gaccgccgcg
    12661 agagcatcgc ccgtgcggga cgggtcctgc ccgccgccct gggcgatgtc gtccttgccg
    12721 ccaccgccgc cgccgagcac gcccgccgcg acccgcacga gcgcgcccgc cttgtggccc
    12781 gcaccgcggg ccgcctcgtt ggtcgcgacg acgatgaccg gccggccgcc acccgaaccg
    12841 gtgaccgcga cgacggccgg ccggtcgttg cccaaccggc cccgcaggtc cagcgccatc
    12901 gtgcgcacgt catccgccgc cgcgtcggag ccggcgtcgt gaccgaggaa ggtcaccccg
    12961 ccgacatcgc gcgccgacgc cgtcaggccg gcgcccgcgg ccgcgagcga ctccttgcgc
    13021 agcttgtcca gttcgcgttc cgcgtccttg agcttgccca tgaggccgtg cacgcgctcg
    13081 ggcaactcct ccggccggcc cttcatcagc tcggtgagct gaccgacgat ggcgcgctcg
    13141 cgggcgagga aggaataggc gtcggtgccc acgagggcct cgacgcggcg tacccccgag
    13201 ccgatggacg actcgccgag cagcgtcacc agacccagct gcgaggtgcg ctccgcgtgc
    13261 gtgccgccgc acagctcgcg cgcccagtcg ccgaccgaga tgacgcgcac ggcgtcgccg
    13321 tacttctccc cgaagagcgc catcgccccg gactcgcgcg cctgcgcctg cgtcatcacg
    13381 tccgcccgga ccgcgaggtc gtcgagcagc acctggttga cccgcgcctc gacgtcgcgc
    13441 agcaccgagg ccggcaccgc gctcgaggcg ttgaaatcga agcggaagcg gcccggtgag
    13501 ttctccgaac cggcctgggt cgcggtgtcc cccaacgctt cccggatcgc cttgtgcacc
    13561 atgtgcgtcg ccgtgtgggc gcgcgagatg gaccggcggc gcgcgatgtc gacttcggcg
    13621 aacgcctgct cgcccgtgcc gacctcgccg tcgacgaccc ggccgcggtg gacgatcaac
    13681 ccggtgatgg gcttctggac gtcgtcgacc tccacgagcg caccattcgc caggcgcagc
    13741 agcccaccgt ccgcgagttg cccaccgccc tcggcataga agggcgtgcg gtcgaggacg
    13801 atctcgacct cctggccgct ggtggccgac gccgcgctga cgccgtcgat gagcaggccc
    13861 gcgacggtcg cttcgcccgt cacgtcctgg tagccggtga actccacctc gtgcccgagc
    13921 gcgtccgcga ccgagcggta tgccgtcgtg tcgccgtgcc cggacttctt cgccttcgcg
    13981 tcggccttgg cccgctgccg ctgctcgctc atcaggcggg tgaacccgtc gcggtcgacc
    14041 tgcagcccct gctcggccgc catctccaga gtcaggtcga tggggaagcc gtaggtgtcg
    14101 tgcagcgcga acgcctggtc gcccgaaagc accgctcccc cagcggattt cgtcttctct
    14161 acggcgacgt cgaggatcgt cgtgcccgcc gcgagcgtgc gccggaaagc ctcctcctcg
    14221 gcatacgcga tctgcgagat ccggccgaac ccgctctcca actcgggata ggacgccttc
    14281 atcttgtcca tcgagatcgg cagcaactcg gggaggcagg gctcgttata ccccagcagg
    14341 cgcatcgagc gcacggcgcg gcgcagcatc cggcgcagca catagccgcg gccctcgttg
    14401 ccgggggtga cgccgtcgcc gatgagcatc agcgcggagc ggacgtggtc ggcgaccacg
    14461 cgcagccgca catcgtcggg gtggctctcg ccggccgagt ggccggagtg gacgccatac
    14521 cgttttccgg tcatctccgc tgcgcgcacg aggacgggct tgacctcgtc gatctcgtac
    14581 atattgtcga cgccctgcag gatcgacgcg atgcgctcca ggcccatccc ggtgtcgatg
    14641 ttcttgttgg gcaattccgc ggcgacgtcg aagtcctcct tggagcgcac cgccgacagc
    14701 tcgtactgca tgaagacgag gttccagaac tccatgaacc ggtcctcgtc gaccg
//
LOCUS       DAOPJG010000070        14645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4306_length_14645_cov_7.812748, whole genome shotgun sequence.
ACCESSION   DAOPJG010000070 DAOPJG010000000
VERSION     DAOPJG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 14645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14645
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4306_length_14645_cov_7.812748"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2681)
                     /locus_tag="PKU75_08175"
     CDS             complement(<1..2681)
                     /locus_tag="PKU75_08175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80890.1"
                     /translation="MPDDALPPAYPSEWEADVVLRDGSVGHVRPITPADEERIRRFHA
                     GQSAESIYLRFFAPLKELSPRDLHRFTHVDYSDRVALVMMHRDDIIGIGRYDKIDPTT
                     AEVAFNISDHYQGKGIGSVLLEHLADIGRDDGLTSFEAEVLPQNHKMLAVFADAGYIV
                     SRRVEDGVVQVHFDIEPTEKSESVRLSREHRAESISISALLAPEVIAVVGAGRTEGSV
                     GHEVLRHLRDGGFTGELYAVNPGADEVLGITSYPTVSAIPSHVDVAVVAVPADQAIAV
                     VEDCAGANVHGLVIISAGFAEAGKDGQKLERRLLRLARDSGMRIVGPTSFGLINTAES
                     VSMNASLAPAMAPAGHLGLFAQSGPLGIAVLQSAGRRSLGISTFASAGNRLDVSGNDL
                     MQYWMDDDRTRAVGLYLESMGNPRKFSRIGRRLAMTKPVIVIKAGASKFGVPPGHRAR
                     VSEVDPVAFTAMLTQAGVIHVDNIHEMFDVAQILIHQPEPKGRRVAIVGNSVQLSALT
                     AQVAESQGLQVTHGPASVDAEAPLDQFAKQVRKALANDEVDSVVVCFTPPLRRGEDEA
                     VAMIHDLAADTDKTVVATMIGTRGLMSRDAPTQPVTETADDAGPDRQLPLYMTPEDAV
                     RALAKATRYGEWRRKDKGMPLSRSGIERGKAQDIIDAHLAENPKGLHLSHEQAQELLA
                     AYGIDLWARTIVASAGEAVAAAETLGYPVVVKSLSPIVRGQASLQGVRVDLGSATAVR
                     EAFESLDARLAAYDRGQFAVQRMAHPGVSTVVTGTEDPLFGPVVTFSVAGPARDLLED
                     IGYRIPPLNDVDVAELIDSIKAAPLLGGYKGAPPVSRGALAELIGRLSMLSDDMSEVA
                     SVILNPVVAHPGGVDVLGAEIELQHPAKRKDSRRRKLT"
     gene            2739..3413
                     /locus_tag="PKU75_08180"
     CDS             2739..3413
                     /locus_tag="PKU75_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPF80891.1"
                     /translation="MDFARLVRARRMTRRFRADAAVPESVVADLLKLAGRAPSAGFSQ
                     GWEYVVLRSPAERETFWATAGPDQPDSRTGWLAGVRRAPVLVLVLADEDAYRRRYAAA
                     DKGGIPPAEQDWPVPYWLTDTAMGALIVLLGATEAGLGSLFFGVPGPRHAAVKDAFGV
                     PAGLDLIGVIALGEEERRVGGSASTRPRRPVSDYVHWGGFGVARAGPEAGLSENAGQT
                     GSAAES"
     gene            3475..6024
                     /locus_tag="PKU75_08185"
     CDS             3475..6024
                     /locus_tag="PKU75_08185"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HPF80892.1"
                     /translation="MARESGPPKDPNDPGDGQVVDIDVSEEMRNAFLEYSVSVIHSRA
                     LPDARDGLKPVQRRILYAMHELGLRPERGHVKSSRVVGETMGKYHPHGDQAIYDALVR
                     MAQPFAMRMTLVDGHGNFGSLDAGPAAARYTEARLAPAAMLMVTGLDEETVDFIPNYD
                     DQFLQPEVLPAAYPNLLVNGATGIAVGMATSMPPHNLVEVISAARHLLAHPNCSLDDL
                     MKFVPGPDLPEGGKIVGLDGIREAYLTGRGTFRTRATAKIEKITPRKMGIVVTELPYL
                     VGPEKVTAKIKELAQAKKLQGISDLKDLTDRKNGLRLVIEIKNGFHPEAVLEQLYRLT
                     PLEDSFGINNVALVGGQPRTLGLKELLEVFVEFRLQVVRRRSEFRLGKRRDRLHLVEG
                     LLIAILDIDEVIQIIRSSDDSDMARTRLMQVFDLSERQAEYILELRLRRLTKFSRIEL
                     EAERDELRAQIEELEKLLADESLLRKLVSSELAEVAKAHGTPRRTVLLESAGAPASAG
                     APLEVADDPCWVLLSSSGLLARTGSADPIPAEGGRAKHDAIAGAVRTSARGHFGIVTS
                     AGRMVRVSALDLPTLVPTSGAPNLAGGTPAKMYVDLARGERILTLAGVEPGGPTLALG
                     TAQGVVKRVVPDYPLGRSEWEVIALKPGDQVVGAAQVPSEQTDLVFISATAQLLRFPA
                     SSVRPQGRAAGGMAGIRLAPKDSVVFFGVVDTGSEGIVVTASGASDALPGTQAGAIKV
                     APYEEYPPKGRGTGGVRAHRFLKGEDVLVLAWAGNTPARASGANGAALELPAATGKRD
                     GSGVAGTAIIAAIAGPVPDATPVLAGGDSGDKTEDAPSQIPAAPERDEPEG"
     gene            6024..7037
                     /locus_tag="PKU75_08190"
     CDS             6024..7037
                     /locus_tag="PKU75_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF80893.1"
                     /translation="MTTIADVAKLAGVSVATVSRTLHGSTRVDPRTRERVVAAAAELH
                     YVASPTATSLASGRTNVVAVVAPMMSRWFFASIVTTIEQALREHGYHTLLIVLGDRPG
                     QEQERLQPEMFVKRVDGILWLNVATTPEETALLERLALPMVCVGAAIDGAPTVLIDDR
                     QAARLAVEHLLGLGHRDIAYLGTAVESTAHMSTPAARVEAFRDSLRAAGLPVHEEWVL
                     DIDWTAAAAAAAIEPVLRGIPRPSAVLAASDEMAIGVLGTARRLGLRVPEDLSVIGID
                     DHTFAGVLGLSTVRQDVPAQGRAAVTMLLEAMNAEDLDGDPVSRIELATSLVPRDTTA
                     PPP"
     gene            complement(7066..8547)
                     /locus_tag="PKU75_08195"
     CDS             complement(7066..8547)
                     /locus_tag="PKU75_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPF80894.1"
                     /translation="MSHAVNEVDERPAGGDTELHRAIGPKLLLLFIVGDILGTGVYAL
                     TGSVAGEVGGAAWAPFLVAFVVATITAFSYLELVTKYPQAAGAALYTHKAFGIHFFTF
                     LVAFTVMCSGITSASTASNAFAGFLRDAFGKKEDWAAGSTPLLLIALAFMAVVAAINF
                     RGVGESVKANVVLTLVELSGLLLVIFIGLWAVTQGKADFSQTMVFDSPSDKSTFFAIT
                     AATSLAFFAMVGFEDSVNMAEECKDPIRDFPRMMLTGLGITGTIYVLVSLTAVALVPV
                     GKLADPGEGSALTQVVAAGAPNMPFDKIFPWIGMFAVANSALINMLMASRLIYGMARQ
                     EVLPPVLGWVHKSRRTPWVAILFTTAIAFGLITYVVRASGSKDGSGTVSLLGGTTALL
                     LLCVFTVVNIAVLILRKDHVGHRHFIAPSVLPIVGAISCAFLAGPWARSEAQQEQYKI
                     AGYLLALGVLLWALTWIANRFFFAKKTYLRDPDELDIHMDTHN"
     gene            complement(8742..10856)
                     /locus_tag="PKU75_08200"
     CDS             complement(8742..10856)
                     /locus_tag="PKU75_08200"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPF80895.1"
                     /translation="MPGASRNTSYTAHHLQVLEGLEAVRKRPGMYIGSTDGRGLMHCL
                     WEIIDNSVDEALAGVCTTITVTLHADGSVEVGDDGRGVPVDIEPRTGLTGVEVVYTKL
                     HAGGKFGGGSYTASGGLHGVGASVVNALSARMDVEVDRGGKTHAMSFRRGEPGVFADQ
                     GAPDPEVPFAPYEETPTLRIVGKVARARTGTRVRYWADRQIFIKDAQFDDEALRSRAR
                     QTSFLVPGLKIVIRDNRGLPGTLGADGPHEEVFHHEGGISEFAEFLATDPPVTDIWRL
                     QGTGTFTETVPVLDAKGHMTPTDVERECGVDIALRWGTGFDTLVASFVNIIATPKGGT
                     HIMGFDQAMLKVFRKQLEVNSRRLKVGTDKVDKDDILAGLTAVVTVRLAEPQFEGQTK
                     EVLGTPAVRQIVARVVEQELTQRFENPKKGEKQQGSLLLEKVVAEMKSRISARLHKET
                     QRAKNALESSTLPAKLADCRSSDTSRTELFIVEGDSALGTAKLARSSEFQALLPIRGK
                     ILNVQKASVADTLKNAECAAIIQVIGAGVGRSFDLGAVRYGKVIIMTDADVDGAHIRT
                     LLITLFFRYMRPLVEAGRLYAAVPPLHRIEVVNPGSKPNDLIYTYSEGEMRQQVAALT
                     KRGRTIKQPMQRYKGLGEMDADQLAETTMDPLHRTLRRVTAEDAQNAAITFELLMGNE
                     VAPRRDFIVAGAARLDRERIDA"
     assembly_gap    10902..11001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11153..11371
                     /locus_tag="PKU75_08205"
     CDS             11153..11371
                     /locus_tag="PKU75_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80896.1"
                     /translation="MNAALAPSLTAADRCDRCGAQAYVRARLHAGGELLFCLHHSREH
                     LPKLRDLAVIDDETERLIETPEFTGEPS"
     gene            11377..11883
                     /locus_tag="PKU75_08210"
     CDS             11377..11883
                     /locus_tag="PKU75_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPF80897.1"
                     /translation="MLSLFGTAGSASIWVPGLLIVAGILGIIVPVIPGLLIAVLGVLI
                     WALDLGTTTGWVVFGICLAIYAAGLAMQFLLPGRRMKAAGIGTSTLLLGALLGIIGFF
                     VIPVIGGPLGFVLGIYLVEMGQSRDRAQAWAQTKVALRGVLHSMGIELIAGMSVLTTW
                     IVGVLITR"
     gene            complement(11979..12386)
                     /locus_tag="PKU75_08215"
     CDS             complement(11979..12386)
                     /locus_tag="PKU75_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HPF80898.1"
                     /translation="MALSEREQQLLDEMEQALYAEDPRFASQMKSVVRRSSHRHHVIG
                     AVGVLAGLGLVLLGVNTTWVVGALGFLVMVASLAYAFAPPRKKAVHLGTVQVDGSVRA
                     SSPKAKRAPKGQRSGGFMERLEERWERRHNSGW"
     gene            complement(12535..13755)
                     /gene="dinB"
                     /locus_tag="PKU75_08220"
     CDS             complement(12535..13755)
                     /gene="dinB"
                     /locus_tag="PKU75_08220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPF80899.1"
                     /translation="MSRRQFKPPPRAGNEPPDDTGCTVLHVDMDAFFASASLLARPDL
                     KGTPVIIGGQTRGVVLSATYEARAFGVTSAMPIARARRLCPQATILRPDHELYSRMSD
                     AVMETFAAITPIVEPLSLDEAFLDISGAVRRLGSPATIAQLIRDTVHDEQGVTCSVGV
                     APTKFVAKLASGLCKPDGLLVVPVAEVVGFVQQLPVGALWGVGEKTEEALGRLGLVTV
                     ADIAHTPLATLKRALGDAGGQHLHELSWGRDPRHVQPVHREKSIGSDETFPVDVDDPA
                     YIHRELLRLSAKTASRMRAAGLVGRTVSIKVRFADFTTITRAKTLRDPTDVTQEIYAT
                     AKSLYDALGLQRARLRLVGVRMEGLVRGANAPVQPTLSEPEFGWRDADRAVDRASARF
                     GSGSVGPASLLRRD"
     gene            complement(13768..14541)
                     /locus_tag="PKU75_08225"
     CDS             complement(13768..14541)
                     /locus_tag="PKU75_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPF80900.1"
                     /translation="MTSERRRPRRGVESGLRTAAVWATVATLADDRRRELGRPLRVLD
                     LGGGTGGMAVPLAQAGHEVIVVDPSPDALASLRRRVDEAGVSDNLRSTQGDADDLRTI
                     LKARVDLVTVHGTLEVIDDPQRALRRISEVVVPGGHLSVVTAQRLAAVLARALAGQFD
                     QARAALESEDGRYGRTDPLPRRFDKAEVEAMVTHAGFTVVDSHGVRLFSDLVPSVYLE
                     SDSERGALVALEQAVATHPEFGILGQIGSALHVVARRHN"
BASE COUNT         2158 a         4860 c         5308 g         2219 t
ORIGIN      
        1 gtcagtttgc gtcgccggct gtccttgcgt ttggccgggt gctgcagttc gatctcggcg
       61 ccgaggacgt cgaccccgcc ggggtgggcg acgaccgggt tgaggatcac cgaggcgacc
      121 tcggacatgt cgtcggagag catcgagagc cggccgatga gttcggcgag cgccccgcgg
      181 ctgacgggcg gggccccctt gtagccgccg agcagtggcg cggccttgat ggagtcgatc
      241 agttcggcga cgtcgacgtc gttgagcggc gggatgcggt agccgatgtc ctcgagcagg
      301 tcccgcgcgg gcccggcgac ggagaaggtg accacgggcc cgaacagggg gtcctcggtg
      361 ccggtgacga cggtcgagac gcccgggtgg gccatgcgtt gcacggcgaa ctggccacgg
      421 tcgtatgccg cgagccgggc atcgagggat tcgaacgcct cccgcaccgc ggtcgcgctg
      481 ccgaggtcga cccgcacacc ttgaagggag gcctggccgc gcacgatcgg cgagagcgac
      541 ttgaccacga cgggatagcc cagcgtctcg gcggccgcga cggcttcccc ggccgaggcg
      601 acgatggtgc gcgcccacag gtcgatgccg taggccgcga ggagttcctg cgcctgctcg
      661 tggctcagat gcaggccttt ggggttctcc gcaagatgcg cgtcgatgat gtcctgcgcc
      721 ttgccgcgct cgattccgct gcgggagagc ggcataccct tgtccttgcg gcgccactcg
      781 ccatagcggg tggccttggc gagggcgcgg acggcgtcct cgggcgtcat atagagcggc
      841 agttgccggt cgggtccggc gtcgtcggcg gtctccgtga ccggctgggt gggggcgtcc
      901 ctcgacatca ggccacgcgt gccgatcatc gtcgcgacca cggtcttgtc ggtgtccgcc
      961 gccaggtcgt ggatcatggc gacggcttcg tcctcgccgc ggcgcagcgg cggggtgaag
     1021 cacacgacga cggagtcgac ctcgtcattg gcgagggcct tgcggacctg cttggcgaac
     1081 tgatcgagcg gcgcctcggc atcgacgctc gccggtccgt gggtgacctg caggccctgg
     1141 ctctcggcga cctgggcggt cagggccgac agttgcaccg agttgccgac gatggccacc
     1201 cgccggccct tgggctctgg ctggtggatg aggatctggg ccacgtcgaa catctcgtgg
     1261 atgttgtcga catggatgac gcccgcctgc gtgagcatcg cggtgaacgc caccgggtcg
     1321 acctcgctca cgcgggcccg atgccctggc ggcacaccga atttggacgc gcccgccttg
     1381 atgacgatga ccggtttggt catcgccagc cggcgcccga tgcgggagaa cttgcggggg
     1441 ttacccatcg attccagata gaggccgacg gcgcgggtgc gatcgtcgtc catccagtac
     1501 tgcatgaggt cgttgccgga gacgtcgagc cggttgccgg cggaggcgaa cgtggagatg
     1561 ccgagggagc ggcgcccggc ggactggagg acggcgatgc cgagcgggcc gctctgcgcg
     1621 aacagcccga ggtggcccgc cggggccatg gcgggcgcca gcgacgcgtt catcgacacc
     1681 gattcggccg tattgatcag accgaaagag gtcggtccga cgatgcgcat gccgctgtcg
     1741 cgggccagcc gcaacaggcg acgttcgagt ttctggccgt ccttgccggc ctcggcgaaa
     1801 ccagccgaga tgatcaccag cccatggaca ttcgcgcccg cgcagtcttc gacgaccgct
     1861 atcgcctgat cggcgggcac ggcgaccacg gccacgtcga cgtgcgacgg gatcgcgctg
     1921 acggtggggt aggacgtgat cccgaggact tcgtcggcgc cgggattgac ggcatacagc
     1981 tcgccggtga acccgccgtc gcgcaggtgc cgcagcactt cgtggccgac cgacccctcg
     2041 gtgcggccgg ccccgacgac ggcgatcacc tcgggcgcca acaacgccga gatgctgatc
     2101 gactcggcgc ggtgctcccg cgacaggcgc accgactccg acttctcggt cggctcgatg
     2161 tcgaagtgga cctgcacgac gccgtcctcg acgcggcggg agacgatata gccggcgtcg
     2221 gcgaacaccg cgagcatctt gtggttctgc ggcaggacct cggcctcgaa actggtcagc
     2281 ccgtcgtcac gcccgatgtc ggcgaggtgc tccagcagca ctgacccgat gcccttgccc
     2341 tggtagtggt cggagatgtt gaacgccacc tcggcggtgg tggggtcgat cttgtcgtag
     2401 cggccgatgc cgatgatgtc gtcgcggtgc atcatcacca gcgccacccg gtcggaatag
     2461 tcgacgtggg tgaagcggtg caggtcgcgc gggctgagtt ccttcagcgg cgcgaagaag
     2521 cgcaggtaga tcgactcggc cgactgcccc gcgtggaagc ggcgaatgcg ctcctcgtcg
     2581 gccggggtga tcggtcggac gtggccgaca ctgccatctc gcagcaccac gtcggcttcc
     2641 cactcgctgg ggtatgccgg gggcagcgca tcatcgggca tgtctccata gttccatgcc
     2701 ggccgccggg cccgggagtg gcttggcaga ctgtcggggt ggatttcgcc aggctggtgc
     2761 gcgcccgccg tatgacgcgg cgcttccggg cggacgcagc cgtgcccgag tcggtggttg
     2821 ccgatctgct caagctggcg ggccgggcac cgagcgcggg tttcagccag ggctgggagt
     2881 atgtcgtgct ccgctccccc gccgaacgcg agaccttctg ggccacggcc ggccccgacc
     2941 agcccgactc gcgcaccggt tggctcgccg gggtgcggcg ggcccccgtc ctcgtcctcg
     3001 tgctcgccga cgaggacgct taccggcggc ggtatgccgc ggccgacaag ggcggcatac
     3061 cgccggccga gcaggactgg ccggtgccct actggctcac cgacacggcg atgggcgccc
     3121 tgatcgtgtt gctcggggcg accgaggcgg ggctcgggtc gctcttcttc ggggtgccgg
     3181 gcccgcggca tgcggcggtc aaggacgcct tcggcgtccc ggccgggctg gacctgatcg
     3241 gggtcatcgc cctcggggag gaggagcggc gggtcggcgg gtcggcgagc acgcgtccgc
     3301 ggcggccggt ctccgactac gtccattggg gcggcttcgg cgtggcccgt gcgggaccgg
     3361 aggccggcct gagcgagaat gccgggcaga ccggctccgc ggccgagtcc tgaccggcgc
     3421 gccggcggga cccggcgagc ggggcgagcc cacgcgaaga gagtgaacga acgcatggcg
     3481 cgcgagagcg gaccgccgaa ggatccgaac gaccccggtg acgggcaggt cgtcgacatc
     3541 gacgtcagcg aggagatgcg caacgccttc ctcgagtact ccgtctcggt catccactcc
     3601 cgcgcgctgc ccgacgcccg cgacgggctg aagccggtcc agcggcgcat cctctatgcg
     3661 atgcacgaac tggggctgcg gcccgagcgc gggcacgtga agtcctcgcg ggtggtcggc
     3721 gagacgatgg gcaagtacca cccgcacggc gaccaggcca tctacgacgc cctggtgcgg
     3781 atggcgcagc cgttcgcaat gcggatgacg ctcgtcgacg ggcacggcaa cttcggctcc
     3841 ctcgacgcgg gtcccgcggc cgcccgctat accgaggccc ggctggcacc cgccgccatg
     3901 ctcatggtga ccggtctcga cgaggagacc gtcgacttca tccccaacta cgacgaccag
     3961 ttcctgcaac cggaagtcct gcccgcggca tacccgaacc tgctggtcaa cggggccacc
     4021 ggcatcgccg tcgggatggc gacctcgatg ccgccgcaca acctcgtcga ggtcatctcg
     4081 gccgcgcggc acctgctcgc ccaccccaac tgcagcctcg atgacctgat gaagttcgtg
     4141 cccgggcccg atctgcccga gggcggcaag atcgtcggac tcgacggcat ccgggaggcc
     4201 tatctgaccg gccgcgggac cttccggacc cgcgccacgg ccaagatcga gaagatcacg
     4261 ccgcgcaaga tgggcatcgt cgtcaccgaa ctgccctacc tggtggggcc cgagaaggtc
     4321 accgccaaga tcaaggagct cgcgcaggcc aagaagctgc agggcatctc ggacctgaag
     4381 gacctgaccg accgcaagaa cgggctgcgg ctggtcatcg agatcaagaa cggcttccac
     4441 cccgaggccg tcctggagca gctgtatcga cttaccccgc tcgaggactc cttcggcatc
     4501 aacaatgtcg cgctcgtcgg cgggcagccg cgcaccctcg ggctgaagga actgctcgag
     4561 gtcttcgtcg agttccggct gcaggtggtg cgccggcgca gcgagttccg gctcggcaag
     4621 cgccgcgacc ggctgcactt ggtcgagggc ctgctcatcg cgatcctcga catcgacgag
     4681 gtcatccaga tcatccgcag cagcgacgac tcggacatgg cccgcacccg gctgatgcag
     4741 gtcttcgacc tctccgagcg gcaggccgaa tacatcctcg agctgcggct gcggcggctg
     4801 acgaagttct cgcggatcga actggaggcc gagcgcgacg agttgcgcgc ccagatcgag
     4861 gagttggaga agctgctcgc cgacgagtcg ctgctgcgca agctggtctc ctccgagctg
     4921 gcggaggtgg ccaaggcgca cgggacgccg cgccgcacgg tgctgctgga gtcggcgggg
     4981 gcgccggcgt cggcgggggc gccgctggag gtcgccgacg acccgtgctg ggtgctgctg
     5041 tccagctccg ggctgctcgc gcgcacgggt tcggcggatc ccatcccggc cgagggcggg
     5101 cgcgcgaagc acgacgccat cgccggggcg gtgcgcacgt ccgcgcgcgg ccacttcggg
     5161 atcgtcacct cggccgggcg gatggtccgg gtctccgccc tcgatctgcc caccctggtg
     5221 cccaccagcg gggccccgaa tctcgccggc ggcacgccgg cgaagatgta tgtcgacctg
     5281 gcccgcgggg agcgcatcct gaccctggcc ggggtcgagc ccggtggccc gacgctcgcc
     5341 ctcggcaccg cgcagggcgt cgtcaagcgg gtcgtgcccg actatcccct cggccgcagc
     5401 gagtgggagg tcatcgccct caagcccggc gaccaggtgg tcggggccgc gcaggtgccg
     5461 tcggagcaga ccgacctggt gttcatcagc gccaccgcgc agctgctgcg cttccccgcg
     5521 agcagtgtgc gcccgcaggg gcgggcggcc ggcgggatgg cgggaatccg gttggcgccc
     5581 aaggactccg tggtcttctt cggggtcgtc gataccgggt cggaggggat cgtcgtcacc
     5641 gcgtcgggtg cctccgatgc gctgccgggc acccaggccg gggcgatcaa ggtcgcgccg
     5701 tatgaggagt atccgcccaa ggggcgcggc accggtggcg tgcgcgccca ccgtttcctc
     5761 aagggcgagg acgtcctggt cctcgcgtgg gccggcaaca cgccggcccg ggcgtccggg
     5821 gcgaacgggg ctgcgttgga gttgccggcg gccacgggca agcgggacgg atccggggtc
     5881 gccgggaccg cgatcatcgc cgctatcgcc ggcccggtgc ccgacgcgac gccggtgctt
     5941 gccgggggcg actccggtga caaaactgaa gatgcaccat cacagatacc ggcggcgccg
     6001 gaacgagacg aacccgaggg ctgatgacca cgatcgccga tgtcgcgaag ctggccgggg
     6061 tctcggtcgc cacggtctcg cgcaccttgc acgggtcgac gcgcgtcgac ccgcgcacgc
     6121 gggaacgggt ggtcgcggcc gcggcggaac tgcactatgt ggcctccccc acggcgacgt
     6181 cgctggcgtc cgggcgcacc aatgtcgtgg ccgtcgtcgc cccgatgatg agtcggtggt
     6241 tcttcgcctc catcgtgacg acgatcgagc aggcgctgcg cgagcacggc taccacaccc
     6301 tgctcatcgt cctcggcgac cggcccggac aggagcagga gcggctccag ccggagatgt
     6361 tcgtcaagcg ggtggacggc atcctctggc tcaacgtcgc gaccacgccc gaggagaccg
     6421 ccctgctgga gcggctcgcg ttgccgatgg tgtgtgtcgg cgcggccatc gacggagcac
     6481 cgaccgtgct catcgacgac cggcaggcgg cccggctggc cgtggagcac ctgctgggcc
     6541 tcgggcaccg cgatatcgcc tatctcggta ccgccgtgga gtcgacggcc cacatgtcga
     6601 cgcccgcggc gcgggtggag gccttccgcg acagcctgcg cgccgccggc ctgccggtgc
     6661 acgaggagtg ggtcctcgac atcgactgga ccgcagcggc ggccgcggcg gcgatcgaac
     6721 cggtgctgcg tggcataccc cggcccagcg cggtgctcgc cgcctccgac gagatggcga
     6781 tcggggtgct gggcaccgcc cggcgcctcg ggctgcgggt gccggaggac ctgtcggtca
     6841 tcggcatcga cgaccacacg ttcgccggcg tgctcgggct gagcacggtg cgccaggacg
     6901 tgcccgccca gggccgggcg gcggtgacca tgttgctcga ggcgatgaac gcggaagacc
     6961 tcgacggcga cccggtcagc cgcatcgaac tcgccacctc cctggtgccc cgggacacga
     7021 cggccccgcc cccctgagcg ggagacgggg ccgggggcga cccggtcagt tgtgggtgtc
     7081 catgtggatg tcgagttcgt ccgggtcgcg caggtaggtc ttcttggcga agaagaaacg
     7141 gttcgcgatc caggtcaggg cccacaggag cacgcccagg gcgagcaggt agccggcgat
     7201 cttgtattgc tcctgctggg cctcgctgcg ggcccacggg ccggcgagga aggcgcagga
     7261 gatcgcgccc acgatgggca ggaccgaggg tgcgatgaag tggcgatgcc cgacgtggtc
     7321 cttgcgcagg atcaacaccg cgatgttgac caccgtgaag acacacagca gcagcaaggc
     7381 cgtggtgcca ccgagcagcg agaccgtgcc ggagccgtcc ttgctgccgc tcgcgcggac
     7441 cacataggtg atcaggccga aggcgatcgc cgtggtgaac aggatggcga cccacggggt
     7501 ccggcgcgac ttgtgcaccc agccgagcac cggcggcagg acctcctggc gcgccatgcc
     7561 atagatcagg cgggaggcca tcagcatgtt gatcagcgcg gagttggcga cggcgaacat
     7621 gccgatccag gggaagatct tgtcgaacgg catgttgggg gccccggccg cgaccacctg
     7681 ggtcagggcc gagccttcac ccggatcggc gagcttgccc acgggcacca gggccacggc
     7741 ggtgagggag accaggacgt agatcgtgcc ggtgatcccc agaccggtga gcatcatccg
     7801 ggggaagtcg cggatcgggt ccttgcactc ctcggccatg ttgaccgagt cctcgaagcc
     7861 gaccatcgcg aagaaggcga gcgaggtggc cgcggtgatg gcgaagaagg tgctcttgtc
     7921 gctggggctg tcgaagacca tggtctggga gaagtcggcc ttgccctggg tgaccgccca
     7981 cagtccgatg aagatgacga gcagcagacc ggagagctcg accagcgtca ggacgacatt
     8041 ggccttcacg ctctcgccga cgccccggaa gttgatggcg gcgacgacgg ccatgaaggc
     8101 gagcgcgatc agcagcaggg gcgtcgaccc ggcagcccag tcctccttct tgccgaaggc
     8161 atcgcgcagg aagccggcga aggcgttgga ggcggtcgag gccgacgtga tgccggaaca
     8221 catgacggtg aaggccacca ggaaggtgaa gaagtggatg ccgaacgcct tgtgcgtgta
     8281 gagcgcggcg ccggccgcct gcgggtactt cgtcaccagc tccaagtagg agaaggcggt
     8341 gatcgtcgcg acgacgaagg cgaccaggaa gggtgcccag gcggcgcccc cgacctcgcc
     8401 ggcgacggag ccggtcaggg cgtagacacc cgttccgagg atgtcaccga cgatgaacag
     8461 caacagcagt ttcggaccga tggcccggtg cagctcggtg tcaccgcccg cggggcgctc
     8521 gtcgacctcg ttcacggcgt gactcatgtc gcgctccctc cgacatccct atccacccgc
     8581 cgggtgcggg tcatgagcgg tcagaatggc agcaaacggg tgccgcgtgt ggtgtgcgac
     8641 acaccatagt tatcgcatct gtatcaaacg accctcccgt cacacgtgtg tgacgggaga
     8701 gccgtcggcg gcgtccttgc cgatccctcc ctggtgccgg atcaggcgtc gatgcgttcg
     8761 cggtcgaggc gggcggcccc ggcgacgatg aagtcgcggc gtggggcgac ctcgttgccc
     8821 atgagcaact cgaaggtgat cgcggcgttc tgggcgtcct cggccgtcac acggcgcagc
     8881 gtgcggtgca gcgggtccat cgtcgtctcg gccagctggt cggcgtccat ctcgccgagg
     8941 cccttatagc gctgcatcgg ctgcttgatc gtgcgtcccc gcttggtcaa cgccgccacc
     9001 tgctggcgca tctcgccctc ggagtaggtg tagatcaggt cgttgggctt gctgccgggg
     9061 ttgacgacct cgatgcggtg caggggtggg acggcggcat acaaccggcc ggcctcgacg
     9121 agcggacgca tgtagcggaa gaacaaggtg atgagcaggg tgcggatgtg ggcgccgtcg
     9181 acgtcggcgt cggtcatgat gatgaccttg ccgtaacgga ccgcccccag atcgaaactg
     9241 cgcccgacgc cggccccgat gacctggatg atcgcggcac actcggcgtt cttgagggtg
     9301 tcggcgaccg aggccttctg gacgttgagg atcttgcccc ggatcggcag cagcgcctgg
     9361 aactcgctcg accgggcgag cttggcggtg ccgagggcgc tgtcgccctc gacgatgaac
     9421 aactccgtgc gtgaggtgtc gctgctgcgg cagtcggcga gcttggccgg cagggtcgag
     9481 gactccaggg cgttcttggc gcgctgggtc tccttgtgca gccgcgcgga gatgcgcgac
     9541 ttcatctcgg cgacgacctt ctcgagcagc agactgccct gctgcttctc gcccttcttg
     9601 gggttctcga agcgttgcgt gagctcctgc tcgacaaccc tggcgacgat ctggcgcacc
     9661 gcaggagtgc cgagcacctc cttggtctgc ccctcgaact gcggctcggc caggcgcaca
     9721 gtcacgaccg cggtcaggcc ggcgaggatg tcgtccttgt cgaccttgtc ggtgccgacc
     9781 ttgaggcggc gggagttgac ctcgagctgc ttgcggaaga ccttgagcat ggcctggtcg
     9841 aagcccatga tgtgggtgcc acccttgggg gtggcgatga tgttgacgaa ggacgcgacc
     9901 aaggtgtcga agccggtccc ccaccgcagc gcgatgtcga cgccgcattc gcgctcgacg
     9961 tcggtgggcg tcatatggcc cttggcgtcg agcaccggga cggtctcggt gaaggtgccg
    10021 gtgccctgca gccgccagat gtccgtgacg ggcgggtcgg tcgcgaggaa ctcggcgaac
    10081 tcgctgatgc cgccctcgtg gtggaagacc tcctcgtggg ggccgtcggc gccgagggtg
    10141 ccgggcaggc cgcggttgtc gcggatgacg atcttcagcc ccggcacgag gaaggaggtc
    10201 tggcgagccc gcgaacgcaa cgcctcgtcg tcgaactggg cgtccttgat gaatatctgc
    10261 cggtcggccc aatagcgcac ccgggtgccg gtgcgggcgc gggcgacctt gccgacgatg
    10321 cgcagggtgg gggtttcttc gtatggcgcg aacgggacct cgggatcggg ggcaccttga
    10381 tcggcgaaga cgccgggctc gccgcggcgg aagctcatcg cgtgcgtctt gccaccgcgg
    10441 tcgacctcga cgtccatccg cgccgagagg gcgttgacga cggacgcgcc caccccgtgc
    10501 aggccgccgg aggcggtgta ggacccgccg ccgaacttcc cgcccgcgtg cagcttggtg
    10561 tagaccacct cgacgccggt cagcccggtg cgcggttcga tgtcgaccgg caccccacgc
    10621 ccgtcgtcgc cgacctcgac cgacccgtcg gcgtgcagcg tgacggtgat cgtggtgcat
    10681 acgcccgcca gcgcctcgtc gaccgagttg tcgatgatct cccacaggca gtgcatcagg
    10741 ccgcggccgt cggtggatcc gatgtacatg cccggacgct tgcggaccgc ctccaggccc
    10801 tcgaggacct gcaggtggtg ggcggtgtag ctggtgttgc ggctcgcgcc gggcacgggc
    10861 ggtgtccttt ctgtctgatc tgcgcctcac cgtaacccgg annnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nataccaaat cgagacgcgc cgcacgccca atctgctggc
    11041 ccgggaacga atcgccgtgt ttgactgttg gaacgagcga cgccgggacg ccccgacgtc
    11101 acccgcatgt aacaccccga gtccacgctc gaggcacaga gagaaggtaa ccgtgaacgc
    11161 cgcattggcc ccgtccttga ccgcagccga ccgctgcgac cgctgtggcg ctcaggcata
    11221 tgtgcgcgcg cggctgcacg ctggtggcga actcctcttc tgcctgcacc actcccgcga
    11281 acacctgccc aagctgcgtg acctcgcggt catcgacgac gagaccgagc gcctcatcga
    11341 gacgccggag ttcaccggcg agccttcctg agcagcgtgc tctccctctt cggcacggcc
    11401 gggagcgcct cgatctgggt ccccggcctg ctgatcgtcg ccggcatcct cgggatcatc
    11461 gttcccgtca tccccggcct gctcatcgcc gtcctcggcg tgctgatctg ggccctcgac
    11521 ctgggcacca ccaccggctg ggtcgtcttc ggcatctgcc tcgcgatcta cgcggccggc
    11581 ctggccatgc agttcctgct ccccggccgc cggatgaaag cggccggcat cggcacctcg
    11641 accctcctgc tcggagcctt gctcggcatc atcggcttct tcgtgatccc cgtcatcggc
    11701 ggcccgctcg gcttcgtcct cgggatctat ctggtcgaga tgggtcagtc ccgcgaccgc
    11761 gcgcaggcct gggcccagac caaggtcgcc ctgcgcgggg tgttgcacag catggggatc
    11821 gagttgatcg ccggtatgtc ggtgctgacc acgtggatcg tcggcgtcct gatcacccgg
    11881 tgagccgagg aacctctgct agcaaaggtt cctgaaatca gcacagctag ggaggtctgc
    11941 ggatttgacg aacctgtgct gagtcggatg cggcgggctt accagcctga gttgtggcgg
    12001 cgctcccagc gctcttcgag gcgctccatg aagccgccgc tccgctggcc cttcggggcg
    12061 cgcttggcct tggggctgct ggcgcggacg ctgccgtcga cctggacggt gccgaggtgg
    12121 acggctttct tgcgcggcgg cgcgaaggcg taggcgagcg aggcgaccat gacgaggaag
    12181 cccagtgcgc cgaccaccca ggtggtgttg acgccgagca ggacgagacc gaggccggcg
    12241 agcacgccca cggcaccgat gacgtggtgg cggtgcgaac tgcggcgcac gaccgacttc
    12301 atctgcgacg cgaagcgagg gtcttcggcg tagagggcct gctccatctc gtcgaggagc
    12361 tgctgttcgc gctccgacag cgccatggtg gcctccccac gggatcggtc ccggccggtg
    12421 gccgggcggt atgcactaaa gacgtccgaa acgggtccgg tagttgacca ttgttaccca
    12481 tttcgctgtg tgtcaaggcc agagtatgtc gcgaatttct tcaagggaag agtgtcaatc
    12541 ccgacgtaac agactggcgg ggcctacact gcccgatccg aaccgcgcgg aggcccggtc
    12601 gacggcccgg tcggcgtcgc gccatccgaa ctccggctcc gacaaggtgg gctggaccgg
    12661 cgcgttcgcc ccgcgcacca gcccctccat ccgcaccccc acgagacgca gccgggcccg
    12721 ttgcaggccc agcgcgtcat acagcgactt ggccgtggca tagatctcct gcgtcacatc
    12781 ggtcggatcg cgcagcgtct tggcccgggt gatcgtcgtg aagtccgcga accggacctt
    12841 gatggagacg gtgcgcccga ccaggccggc agcgcgcatc cgtgaggcgg tcttggcgga
    12901 gagccgcagc agctcacggt ggatatatgc cgggtcgtcg acgtcgacgg ggaaggtctc
    12961 gtcggagccg atggacttct cgcgatgcac cggctggacg tggcggggat cgcgccccca
    13021 ggacagctcg tgcaggtgct gcccgccggc gtcgcccagg gcgcgcttga gggtggcgag
    13081 gggggtatgg gcgatgtcgg cgaccgtcac caggccgagc cgccccaggg cctcctcggt
    13141 cttctccccc accccccaca aggcgccgac gggcagttgt tggacgaatc cgacgacctc
    13201 ggcgacgggg accacgagca ggccgtcggg tttgcacaga ccggaggcga gcttggcgac
    13261 gaacttggtg ggggcgacgc cgacggagca ggtgacgcct tgttcgtcgt ggacggtgtc
    13321 gcggatgagc tgggcgatgg tcgcggggct gcccaggcgg cgtactgccc cgctgatgtc
    13381 gaggaaggcc tcgtcgaggc tgagcggctc gacgatcggg gtgatggcgg cgaaggtctc
    13441 catcaccgca tccgacatcc gggagtagag ctcgtgatcg gggcgcagga tcgtcgcctg
    13501 cgggcacagc ctccgggcgc gggcgatggg catggcggag gtgacgccga aggcgcgggc
    13561 ctcgtaagtg gccgacagga cgaccccgcg ggtctgcccg ccgatgatga cgggggtgcc
    13621 tttgaggtcg gggcgcgcga gcagggaggc ggaggcgaag aaggcgtcca tgtcgacgtg
    13681 caggacggtg cagccggtgt cgtcgggcgg ctcgttgccc gctcgcggcg ggggcttgaa
    13741 ctggcggcgg ctcatcggcg cgctccgcta gttgtggcgg cgggccacca cgtgcagggc
    13801 ggacccgatc tgtccgagga tgccgaactc ggggtgcgtg gccacggcct gctcgagcgc
    13861 gacgagggcg ccccgctcgg agtcggactc gagatagacc gacggcacca ggtcgctgaa
    13921 caggcgcacg ccgtggctgt cgacgacggt gaagccggcg tgggtgacca tcgcctcgac
    13981 ctcggccttg tcgaagcgtc gcggcagggg gtcggtgcgg ccgtagcggc cgtcctcgct
    14041 ctccaaggcg gcgcgcgcct ggtcgaactg accggcgagg gcccgggcga ggaccgcggc
    14101 gagacgctgg gcggtgacga ccgagaggtg ccctccgggg accacgacct cgctgatacg
    14161 ccgcagcgcc cgctgcgggt catcgatcac ctccagcgtg ccgtggacgg tgaccaggtc
    14221 gacgcgggcc ttgaggatcg tgcgtaggtc gtcggcgtcg ccctgcgtgg aacgcagatt
    14281 gtcgctgaca ccggcctcgt cgacccggcg gcgcagggac gcgagcgcgt cggggctcgg
    14341 gtcgacgacg atgacctcgt gaccggcctg ggcgaggggc acggccatac cgcccgtgcc
    14401 gccgccgaga tcgagcaccc gcagcggccg accgagctcg cgccggcggt cgtcggcgag
    14461 ggtcgcgacc gtcgcccaca cggcggccgt gcgcaacccg ctctccactc cgcgccgggg
    14521 gcggcggcgt tcgctcgtca tgctcaaact gtatgcggtg cccccgacac ctcgcggcgc
    14581 gatccccgag gcgcgggaga cggggcgcgc ggagagccgg gccgggttcg acggaagcgg
    14641 ggcct
//
LOCUS       DAOPJG010000071        14545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4351_length_14545_cov_6.977226, whole genome shotgun sequence.
ACCESSION   DAOPJG010000071 DAOPJG010000000
VERSION     DAOPJG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14545
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4351_length_14545_cov_6.977226"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKU75_08230"
     CDS             complement(<1..439)
                     /locus_tag="PKU75_08230"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPF80901.1"
                     /translation="MTRFRTALAASAGALALSVTPVALAATGDAPRGCKSFEATLAQH
                     LNAIGDRDLAALEPTVDDSVTLIFPSGRIRDGKEAFMAFHEAWFADLTWRQPVEVIRT
                     NVQGCKTAWVLVDYHYQDLDDAGNVISDSHAMFALTWTFKQGRW"
     gene            537..1586
                     /locus_tag="PKU75_08235"
     CDS             537..1586
                     /locus_tag="PKU75_08235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPF80902.1"
                     /translation="MDACPALRRRESAGRVRATRYVALGDSYAAGVGGAARRNACWRA
                     DDGYPVQVARRLGLDVAYNACLGAVVADVLAHQLAPLGPDTTHVSVTVGGNDIGFVPV
                     LIAAAEPGWMANSDVSIDHALIAMRQVLPGRLDQLFAEVTGRAPNAYVVATAYPRLFN
                     GVDCNLATFFSPHEMERLNAAADELGSVIAAAARRAGIRYAGVGTRFAGHAVCDDPEW
                     INGVSWPVEGSFHPNSLGHNAYADVVASALAAKGISPEAGAAVEIVEGPCVPGSAPTF
                     SIPDLLSARSLDGAREYGLDPAEVERLARQLYAGLDAAQGALRPSEETYAAAARLAEL
                     DAVARARRGEVKMDG"
     gene            1579..>2129
                     /locus_tag="PKU75_08240"
     CDS             1579..>2129
                     /locus_tag="PKU75_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80903.1"
                     /translation="MAEAGADEAGEARLAAAVGRLYAVPLADFMATRTSLVAQAKADK
                     DKALAARLGKLRKPSAAAWTLNQLVRARPDLVEEIESVGAQLRAAQANLHGTAITALR
                     PARDQLIADVLAGAGEISAGGGSSLSGPAADEIRDSVIAALASAEASEAVTSGALTRA
                     LHYSGFGEVDLEDAVVATTTGRLL"
     assembly_gap    2130..2229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2230..2590
                     /locus_tag="PKU75_08245"
     CDS             <2230..2590
                     /locus_tag="PKU75_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80904.1"
                     /translation="EDESDEEPEPEDEPEPEDEPEEQGPVEEGDPEALIEEAAQAYAQ
                     AAAEVARAKAAVRESSERLDAIKGRIAELGAELARAEAESEELFAADAQAREAMAAAV
                     KTRQAAAELLAKREADG"
     gene            2590..4014
                     /locus_tag="PKU75_08250"
     CDS             2590..4014
                     /locus_tag="PKU75_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPF80905.1"
                     /translation="MPTDPRGLRSEAPLLDAWLAFQENTSHAFTIPGHKHRIDLVGDV
                     IAGDVPLYAGLDTMKVTEGTLAEAERRAARLWGADRCRFSVGGSTHGNQALALAVGAP
                     GDRVVLTRSAHRSLVTALVLAGLEPVWVTPDVDPQTGLPLPIAVEAVETACAAADSRR
                     LAGVFLTDPAYVGTHGDIAAWADVAHAHDVPLIIDAAWAAHFGFHPDLPPHALAAGAD
                     ALVTSAHKALPAYSQGALILVRGNRIDADRLEAAVEATHTTSPAGSILASIDASRALL
                     ERDGAELVGAVLRAVRATRDRLAGVPGLLVLDGPGVDPLKLTLVLSGTGADGVAVENR
                     LLDRGRPVEMADRDTIVAMVTLADTAEDLESLADNMIAAIEAERGEPHEPAGMAAYRV
                     VPEPVRTPREAFFARREVVESEAAVGRVSAELIAPYPPGIPVLAPGERITPAVLTALR
                     EAQTSGARIAYASDPTLASVLVLR"
     gene            complement(4067..5095)
                     /locus_tag="PKU75_08255"
     CDS             complement(4067..5095)
                     /locus_tag="PKU75_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF80906.1"
                     /translation="MDFKFTDEQDSLREAARDAIGRNRTEPVPGDAVHNEKMWQALAE
                     LGALGLPFPEDAGGMGAGPIEAMIVAAELGRVGARTAYADTLMAVTLLGQDELAGAAM
                     EGSRLVVPALYEPMRAWSPEPASVTTDGSALEGVREPVPFAAAAPAVVTTARGDDGIA
                     VYAIDSPTFDGARLDLAGQSARHVGGDAHLRQALNLGTLVLAGEALGAMESALGLTVE
                     YLKSRKQFGVPLMKFQTLTQRAADMYTSLELARSAIYFGAMSLAEDPEDDLTISRMKV
                     ITGKAGKHIGQEAIQLHGGIGMTAEYAVGHYTARLTEIEHSLGDTRYHLGRLAARVKD
                     YGALDLVG"
     gene            complement(5102..6283)
                     /locus_tag="PKU75_08260"
     CDS             complement(5102..6283)
                     /locus_tag="PKU75_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF80907.1"
                     /translation="MERGLSAEEAQFRQEMREFFTTKVPQEIRDKVAAGEELTKEDLA
                     TSHKILNEHGYAVPQWPVEWGGQDWSDLQRYIWLDEMQLANVPAPLPFNANMIGPVIA
                     TFGSEEMKKRFLPPTAALDIWWCQGFSEPESGSDLASLRTTAVRDGDEYVVNGQKTWT
                     TLGQHADWIFNLVRTDPNAPKQQQGISMLLIDMKTPGVTVRPIQLIDGGHEVNEVFFE
                     NVRVPADQLVGEENRGWDYAKFLLGNERVGVAPTGWIKRKLASAKEYAASVAAGDGTL
                     LDDPLVSARIAELECDLAALELTVLRVAANSHGGKPDPASSILKLRGSELQQDVLELI
                     ADIAGPSSLAWSDADGSEVPEWAHNATPVYLNFRKASIYGGSNEVQRGVISSGILGLK
                     G"
     gene            6512..6907
                     /locus_tag="PKU75_08265"
     CDS             6512..6907
                     /locus_tag="PKU75_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPF80908.1"
                     /translation="MSEAPAEKADAVRVSRVLNHPVTSVWGALMAPHGAEALLGEGGE
                     LGSKGESWHAADGTYGVTRSFHPMEQIRFSWHADADAPATLVDVHLRPEGEQTDLEIV
                     HDHLDEAADRDWLAAHWESALDRLDSMSI"
     gene            complement(6958..7659)
                     /locus_tag="PKU75_08270"
     CDS             complement(6958..7659)
                     /locus_tag="PKU75_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPF80909.1"
                     /translation="MAWFGRGRGEAKGLSRLAQAGGPVGAYAAAGLPDPATPAAHLRL
                     LALDVETTGLDPAKDHILSVGFVPVSGRAITLDGAGQVHVRPPDGHEGVGASATVHGL
                     TDDQVAEGVSAREALDLVAAALTGRVLLAHHAPIEVGFLSAVCRAAYGCDLPVRVIDT
                     LALAQRAYPAHVEPPPGALRLWSARARFGLPVYGAHDALMDALACGELYLAQLAELEA
                     TSSRPVTLARLASSP"
     gene            complement(7686..9554)
                     /locus_tag="PKU75_08275"
     CDS             complement(7686..9554)
                     /locus_tag="PKU75_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HPF80910.1"
                     /translation="MALDVELAEIRDFLAAQEPFADLPQEALTRLVPRLTVEYHRRGT
                     AIMTPGRDSVHLYVVRSGAVDMRDAIGTLVDRGEVGDCFGSTTLLGGNPSTFSVVAIE
                     DTLTLAMGAATFAELCNDHREFGEFFDAQRTRRMAGAVAAKQASASGSAILKTRMSEL
                     ARKAPITVSVRANVREAAQVMSDAGVSSLLVMDGERLAGIITDRDLRNRVLAAGRDGT
                     TPLADVMSANPVTARADGLAFEAMLEMVARNIHHLPLMDGDRAIGVVTTTDLMRLEQA
                     NPVYIAGDIAKQKDSAGVATAAARLPRVVESLVAQDASAADMGRIVTSVGDAVERRLI
                     ALAEERLGAPPVPYCWVTLGSRARMEQALAADQDNALIIDDAAQPADLDYFAQFAELV
                     TGWLVEAGYPRCPGDVMATNPKWRITLREWKHVFGEWLNAPVPDAILRASIFFDMRPV
                     AGDAQLYAELDDLVRSQAPSSKRFLSHLAGAAVANEPPVGFFRGFVLEKEGEHAHTLD
                     IKRGGVGAVVDLARVHALALGLPARNTDERIAGAVAAKVMGAARGADLRDAFEFISYV
                     RMSHQAAQVKAGTPPDNHISPDELSSFDKRHLREAFGIVRSAQSTLGTVYGTGKLG"
     gene            9690..10259
                     /locus_tag="PKU75_08280"
     CDS             9690..10259
                     /locus_tag="PKU75_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF80911.1"
                     /translation="MTPRAPALAPDDRRAAIVAATLPLVLAEGRAVTTRQIAEAAGVA
                     EGTIFRVFATKDDVIDAVIEAALDMEPFIAALEALPSPASLDEAVHWLAQAQLERFAQ
                     VFSLMTVLGIHGPPKHQPRPEWIARLAAAQMEVLAPFAEELTLPPREVVRYIRLLAFA
                     GGNSHITDGELLTAAGITTLVLDGSRKKG"
     gene            10265..11998
                     /locus_tag="PKU75_08285"
     CDS             10265..11998
                     /locus_tag="PKU75_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80912.1"
                     /translation="MLWRLIRTHLGPYAGAVAAILTLQLVSTATTLYLPSLNGQIIDD
                     GVALGDTGEIMRLGAIMLAVSLVNILSTVAIARQASATSAGMASDLRREVFGRVTSFS
                     AQEVSRFGAPTLISRNTNDVQQVQLVVNMGLMFMMSIPIMMIGGIFMALREDVGLSWL
                     VAVSVPLLGIIVGSIVVKMLPWFRRMQESVDAVNRILREQITGIRVVRAFVREDVEEK
                     RFDDANTTYTGTAVAVGKLFSSVFPLVMFVFNLASVAVWWFGAKRVDAGQMEVGAVTA
                     FMQYLMQILMSVMFATMMSMMIPRASVSAGRITEVLQTPSSVIDTPDAPGLEDGRLPV
                     RFDMVDFSYPGAEQPVLHDITFQANPGQTTAIIGSTGSGKTTLVDLIPRLYDVTDGSV
                     RIGDRDVREVPLEDLWRHLGLVPQRPFLFSGTVASNLRYGDEEATDEQLWEALRIAQA
                     EDFVSAMPGGLDAPISQGGTNVSGGQRQRLAIARALVRRPAVYLFDDAFSALDVATDA
                     RLRAALRPHTRDATVIIVAQRVSSIMHADQIIVLDDGRVVGIGGHEQLLASCPTYAEI
                     VASQALTEEAA"
     gene            12001..14013
                     /locus_tag="PKU75_08290"
     CDS             12001..14013
                     /locus_tag="PKU75_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF80913.1"
                     /translation="MADRILTEEEKAAEQRRGAAGSAQRSGPPHMRMGQPGEKSRNFG
                     PSARRLIGLMGRDKPLLWLALAFALVSVILNALGPKILGRATDYVFSGAVGRVLPDGM
                     TVEQAVAFLRAQGKDQVADMVSGMKYLVPGQGVDFTALGRILLIVLALYAVASLLSWV
                     QARIIVRVVNGAVYRLRRDVEEKLSRLPLSYFDSQPRGELLSRVTNDVDNIGQSLQQT
                     LSQLVTSFLTVMAMLVMMLWVSPLLAIIALVTIPVSIGITAVIGKRSQAHFVQQWKST
                     GEVNTIVEETFTGHGLVKVFNRGAQASADFGVKNEELRKATFGAQFVSGLIMPMMMFV
                     GNLNYVIIAVVGGLRVAHGQMSIGDVQAFIQYSRQFTQPLTQVASMANLMQSGVASAE
                     RVFEVLDAPEQIADAADAVHVEDPHGRVAFEDVSFSYTPDRPLIEHLDLVVEPGQTVA
                     IVGPTGAGKTTLVNLIMRFYELNGGRITLDGIDIASMRRADLRGEIGMVLQDTWLFEG
                     TIRENIAYGKPGASDAEVRAAAEATFVDRFVHSLPDGYETKLDSEAGNISAGEKQLLT
                     IARAFISDPALLILDEATSSVDTRTELLVQHAMQALRQDRTSFVIAHRLSTIRDADLI
                     LVMEDGRIVEQGSHAELLAAGGAFARLYAAQFAGAPDAEDAAAPIA"
     gene            14162..>14545
                     /locus_tag="PKU75_08295"
     CDS             14162..>14545
                     /locus_tag="PKU75_08295"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPF80914.1"
                     /translation="MAEAYLLSLDVQGARERLVSAREEERVRLRNDLHDGLGPLLVGA
                     RMQLAAAARAGSDARRADLVAGASADLGTASTSVRELIDGLRPAALDGGLVTALEEAA
                     HGLLPDCEVVLDAPAELAPMPPAVEI"
BASE COUNT         2098 a         5070 c         5016 g         2261 t
ORIGIN      
        1 cccagcggcc ctgtttaaag gtccaggtga gcgcgaacat cgcgtgcgag tcggagatga
       61 catttccggc gtcgtcgagg tcctggtagt ggtagtcgac cagcacccac gcggtcttgc
      121 agccctggac gttggtgcgg atcacctcga cgggctggcg ccacgtgagg tcggcgaacc
      181 aggcttcgtg gaaggccatg aaggcctcct tgccgtcgcg gatgcgtccg ctcgggaaga
      241 tcagcgtgac ggagtcgtcg acggtgggct ccaacgccgc caggtcgcga tcgccgatcg
      301 cgttcaggtg ctgggccaag gtggcctcga agctcttgca gccccgcggc gcgtcaccgg
      361 tggctgccag ggcgacgggc gtgacggaca gggcgagcgc tcctgcgctc gcggcgaggg
      421 cggtgcggaa gcgggtcatc gtcatcctcc tgctgtttcg atgagcggac cccccgagcc
      481 caccaccgcg tggggcacgg cgcgagcctt tccgcctggg tcgaaaggtt ctccgcttgg
      541 acgcctgccc ggcgttgcgt cgcagagagt cggcaggcag ggttagggcc acgcgatatg
      601 tcgcgctggg agattcgtat gccgcgggcg tcgggggggc agcgcgccgc aatgcgtgct
      661 ggcgcgccga tgacggctac cccgtgcagg tggcccggcg gctcggtctc gacgtcgcct
      721 acaacgcgtg tctcggtgcc gttgtcgccg atgtcctggc gcaccagctg gccccgctcg
      781 ggccggatac gacccatgta tcggtcaccg tgggcggcaa cgacatcggc ttcgttcccg
      841 tgctcatcgc cgccgccgaa ccgggctgga tggccaactc cgatgtcagc atcgatcacg
      901 ccctgatcgc gatgcggcag gtgctgccgg ggcgtctcga ccagctcttc gccgaggtga
      961 ccggtcgcgc gccgaacgcg tatgtcgtgg cgacggcata cccgcggctc ttcaacggtg
     1021 tcgactgcaa tctcgcgacc ttcttctcac cacacgagat ggaacgactc aacgccgccg
     1081 ccgacgaact cggttcggtc atcgccgctg ctgcccgccg ggcgggcatc cggtatgccg
     1141 gtgtcgggac ccggttcgcc ggtcacgctg tgtgcgacga ccccgaatgg atcaacggcg
     1201 tcagctggcc cgtcgaggga tcgttccacc cgaactcgtt ggggcacaac gcatacgccg
     1261 acgtggtcgc gtccgccctg gcggccaagg ggatctcgcc cgaggccggg gctgccgtgg
     1321 agatcgtcga aggtccgtgc gtgcccggct cggcgccgac cttctccatc cccgacctgc
     1381 tgagcgcccg gagcctcgac ggcgcccgtg agtacggcct ggatcccgcc gaggtggagc
     1441 gcctcgcccg tcagctgtat gccggcctgg atgccgccca gggcgccctg cgccccagcg
     1501 aggagacgta tgccgccgcg gcccggctcg ccgaactcga cgccgtcgcg cgcgcccggc
     1561 gcggggaagt gaagatggat ggctgaggcc ggcgctgacg aggcgggcga ggcccggctc
     1621 gccgccgccg tcgggcggct gtatgccgtc ccgctggccg acttcatggc cacacgcacc
     1681 tcgctggtcg cccaggccaa ggcggacaag gacaaggcgc tcgccgcgcg cctggggaag
     1741 ctgcgcaaac cgagcgccgc ggcctggacc ctcaaccagc tcgtccgggc ccggcccgat
     1801 ctggttgagg agatcgagag cgtcggagcg caattgcgtg cggcacaagc caatctgcac
     1861 ggaacggcca tcacggcgct gcgccctgcg cgcgaccaac tcatcgccga tgtgctcgcc
     1921 ggcgccggcg agatcagcgc cggcggcggc agttcgctca gcggacccgc cgccgacgag
     1981 atccgggatt cggtgatcgc ggccctggcg agtgccgagg cgtccgaggc ggtcacgtcg
     2041 ggtgccctga cccgggccct gcactattcg gggttcggtg aggtggacct cgaggacgcc
     2101 gtcgtcgcca ccacgaccgg ccggctgctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnt gaggatgagt ctgatgagga gcccgagccc gaggacgagc cggagccgga
     2281 ggatgagccc gaggagcagg ggcctgtgga ggagggggat ccggaggccc tgatcgagga
     2341 ggcggcgcag gcctacgcgc aagccgccgc ggaggtcgcg cgcgccaagg ctgcggtgcg
     2401 agagagcagc gaacgtctcg acgcgatcaa ggggcgcatc gccgagctcg gcgcggagct
     2461 ggcgcgtgcc gaggccgaga gcgaggagtt gttcgccgcc gacgcccagg cccgggaggc
     2521 aatggccgcc gcggtcaaga cccggcaggc cgcggccgag ttgctcgcca agcgcgaggc
     2581 cgacggctga tgcccaccga cccgcgcggt ctgcgctccg aggcgcccct cctcgacgcg
     2641 tggttggcct tccaggagaa cacctctcac gccttcacca tcccggggca caagcaccgc
     2701 atcgacctgg tcggcgacgt gatcgccggt gatgtgcccc tctatgcggg gctcgacacg
     2761 atgaaggtga ccgagggcac gctcgccgag gccgaacgac gcgccgcccg gctctggggc
     2821 gccgaccggt gccggttctc ggtcggcggt tcgacccacg gcaaccaggc cctcgcactg
     2881 gccgtgggcg ccccgggtga ccgcgtcgtg ctgacccggt cggcgcaccg ctcgctcgtc
     2941 accgctctcg tgctcgccgg gctcgagccg gtctgggtga cccccgacgt tgacccgcag
     3001 accggcctgc ccctgccgat cgccgtggag gctgtcgaaa cagcctgtgc tgcagcagat
     3061 tctcggcgcc tcgccggtgt cttcctgacc gatccggcgt atgtcggcac ccacggggac
     3121 atcgccgcgt gggccgacgt cgcccacgcc cacgacgttc cgctgatcat cgacgcggca
     3181 tgggccgcgc acttcggttt ccatcccgac ctgccgccac acgctctcgc cgccggtgcc
     3241 gacgcgctgg tgacgagcgc gcacaaggcg ctcccggcat acagtcaagg tgcgctgatc
     3301 ctggtgcgcg gcaaccggat cgacgcggat cgcctcgagg ccgcggtcga ggcgacacac
     3361 acgacgagtc cggccggctc catcctcgcg agcatcgacg ccagtcgcgc gctcctcgaa
     3421 cgcgacggcg ccgaactcgt gggcgcggtc ctccgagccg tccgtgcgac ccgggaccgg
     3481 ctggcgggcg tgccgggact gctcgtcctc gacggcccgg gtgtcgaccc gctcaagttg
     3541 accctggtgc tgagcggcac cggcgccgac ggtgtcgccg tcgagaatcg tctcctggac
     3601 cgtggccgtc cggtcgagat ggccgaccgc gacaccatcg tcgcgatggt gacgctcgcc
     3661 gacacggccg aagacctgga atcgttggcg gacaacatga tcgctgccat cgaggcggag
     3721 cgcggcgagc cgcacgagcc ggccgggatg gcggcatacc gtgtcgttcc cgaaccggtc
     3781 cgcactccgc gcgaggcctt cttcgcccgg cgagaggtcg tcgaatccga ggcggcggtc
     3841 gggcgggtga gcgccgaact catcgcgccc tacccgcccg gcatacccgt cctcgccccc
     3901 ggcgagcgca tcaccccggc ggtcctcacg gccttgcgcg aggcgcagac ctccggcgcc
     3961 cggatcgcct acgcctccga ccccacgctg gcgagcgtcc tggtgcttcg ttgagtccgg
     4021 cgtccggcgg gcggtgaccg ccgaacgccg gacccaacgt gagggttcag ccgaccaggt
     4081 cgagggcccc gtagtccttg actcgcgcgg cgagccggcc caggtggtag cgggtgtctc
     4141 cgaggctgtg ctcgatctcg gtgaggcggg cggtgtaatg gcccacggcg tattcggcgg
     4201 tcatgccgat gccgccgtgc agctggatcg cctcctggcc gatgtgcttg ccggccttgc
     4261 cggtgatgac cttcatccgc gagatcgtca ggtcgtcctc ggggtcctcc gccaggctca
     4321 tcgcgccgaa atagattgcg ctgcgggcaa gttcgagcga ggtatacatg tccgcggcgc
     4381 gctgggttag ggtctggaac ttcatcagcg ggacgccgaa ctgcttgcgg ctcttgagat
     4441 attcgaccgt caggccgagc gccgactcca tcgcgccgag agcctcaccg gcgagcacga
     4501 gcgtgccgag gttgagggcc tgccgcaggt gggcatcgcc gccgacgtgg cgggcgctct
     4561 gcccggcgag gtcgagccgc gcaccgtcga aggtcggcga gtcgatcgcg tagacggcga
     4621 tgccgtcgtc gccgcgggcg gtggtgacga ccgccggtgc ggcggcagcg aagggcaccg
     4681 gctctcggac gccctccagg gcgctgccgt ccgtggtgac actcgccggc tcgggcgacc
     4741 aggcacgcat cggctcatag agggcgggca caacgagccg gctgccctcc atcgcggcgc
     4801 cggccagctc gtcctggccg aggagggtga cggccatcag cgtgtctgcg tatgccgtcc
     4861 gcgcacccac gcgtcccagc tcggcggcca cgatcatcgc ctcgatgggt ccggcgccca
     4921 tgccgccggc gtcctcgggg aagggcaggc cgagtgcgcc gagttcggcg agcgcctgcc
     4981 acatcttctc gttgtgcacg gcgtcgccgg ggacgggttc ggtgcgattg cggccgatcg
     5041 cgtcgcgggc ggcctcgcgc agggagtcct gctcgtcggt gaacttgaaa tccattgctg
     5101 ctcagccctt cagcccgagg atgccactgg agatgacgcc gcgctggacc tcattggatc
     5161 cgccatagat cgacgccttg cggaagttga gatagaccgg ggtggcgttg tgcgcccact
     5221 ccggcacctc gctgccgtcc gcgtcgctcc acgccaggga ggacggaccg gcgatgtcgg
     5281 cgatcagctc gaggacgtcc tgctgcaact cgctcccgcg cagcttgagg atggaggacg
     5341 ccggatcggg cttgccgcca tgggagttgg cggcgacccg caacacggtc agctcgagcg
     5401 ccgcgagatc gcactccaac tcggcgatac gggccgagac cagcgggtcg tcgaggagag
     5461 tgccgtcgcc ggcggcgacg gacgcggcat actccttggc ggacgccagc ttgcgcttga
     5521 tccacccggt cggggccacg ccgacgcgct cgttgccgag caggaacttc gcatagtccc
     5581 aaccccggtt ttcttctccg acaagctgat ccgcgggcac gcggacgttc tcgaagaaaa
     5641 cttcgttgac ctcgtggccc ccgtcgatga gctggatcgg ccgcaccgtg actccagggg
     5701 tcttcatatc gatcagcagc atcgagatgc cctgctgttg cttgggggca ttgggatcgg
     5761 tgcgcacgag gttgaagatc cagtcggcgt gctggcccaa ggtggtccac gtcttctggc
     5821 cgttgacgac atactcatcg ccgtcgcgga cggcggtcgt gcgcagcgag gcgaggtcgg
     5881 agccggactc cggctcggag aagccctggc accaccagat gtcgagcgcg gcggtcggtg
     5941 ggaggaagcg cttcttcatc tcctccgacc cgaaggtggc gatgaccggg ccgatcatgt
     6001 tcgcgttgaa cggcaagggt gcggggacgt tcgccagctg catctcgtcg agccagatgt
     6061 agcgctgcag atcactccag tcctggccgc cccactcgac gggccactga gggacggcat
     6121 acccgtgctc gttgaggatc ttgtgggagg tcgcgaggtc ctccttggtc aactcctcgc
     6181 ccgccgcgac cttgtcgcgg atctcctgcg ggaccttcgt ggtgaagaat tcccgcattt
     6241 cctggcggaa ttgcgcctcc tcggcgctca agccacgctc catcagctgc tctccgtcgt
     6301 catcgatcgg tccgccggcg agcgccggcc gcttccccta ttcaacaacg cgcgactaag
     6361 cgaccgctca gcgcggggct atgacgttgg gcacgagccg gctgtttccc gctgtcggct
     6421 ccaaggcgta gggtcagaaa gcacaggccg gcaccggccg gcctcctgcc accgatggtg
     6481 aaaggacgtg gcatcgatga cgatggacac cgtgagcgag gcacccgccg agaaggccga
     6541 tgccgtgcgc gtcagccggg tgctcaacca ccccgtcacg agcgtctggg gcgcgctgat
     6601 ggccccccat ggcgccgaag ccctgctggg cgagggcggc gagctcggat ccaagggcga
     6661 gagctggcac gcagccgacg gcacctacgg cgtgacccgc agtttccatc cgatggagca
     6721 gatccgcttc agctggcacg ctgacgccga cgctcccgcc accctggtcg acgtgcacct
     6781 gcgtcccgag ggcgagcaga ccgacctcga gatcgtccac gaccacctcg acgaggctgc
     6841 cgaccgcgac tggctcgccg cgcactggga gtcggccctc gaccgcctgg actcgatgtc
     6901 catctgatct cccggcgaag cctgatcttt ccgcgaagcc gccccggcac tgcgtcgcta
     6961 cggggagctc gcgagccggg cgagcgtgac cgggcgcgaa ctggtcgcct ccagctcggc
     7021 cagctgggcg agatagagct cgccgcacgc gagcgcatcc atcaaggcgt cgtgggcgcc
     7081 gtacaccggt aggccgaagc gcgcccgggc gctccacagc cgcagcgccc cgggcggcgg
     7141 ctcgacgtgc gccggatagg cgcgctgggc gagggccaag gtgtcgatga cgcggacggg
     7201 caggtcgcag ccgtatgccg cgcggcatac cgccgagagg aagccgacct cgatcggggc
     7261 gtggtgcgcg aggaggacgc ggccggtcag tgccgcggcc acgaggtcca gggcctcccg
     7321 ggcgctgacg ccctccgcga cctgatcgtc ggtgagcccg tgaacggtcg cgctcgcgcc
     7381 gacgccctcg tggccgtcgg gcgggcgcac gtgcacctgt ccggcgccgt ccagggtgat
     7441 ggcccggccg ctgaccggga cgaatccgac actgaggatg tggtccttcg ccgggtcgag
     7501 cccggtcgtc tcgacgtcga gggccagcag gcgcaggtgt gcggccgggg tggcggggtc
     7561 ggggagcccg gctgcggcat acgccccgac ggggccgccg gcctgggcca gccgcgagag
     7621 gcccttggct tccccgcgtc cccgcccgaa ccacgccatc agttcccccc ggtgagcacg
     7681 ccggctcagc cgagcttccc ggtgccgtag acggtgccga gggtcgactg agccgagcgg
     7741 acgatcccga acgcctcacg caggtgccgc ttgtcgaagg agctcaactc gtccgggctg
     7801 atgtggttgt cggggggtgt gcccgccttg acctgggcgg cctggtgact catccggaca
     7861 tacgagatga actcgaaggc atcccgcagg tccgctcccc gcgcggcacc catgaccttc
     7921 gccgccacgg caccggcgat gcgttcgtcg gtgttgcggg ccggcagtcc gagggccaga
     7981 gcgtggaccc gggcgaggtc gacgaccgcg ccgacgccgc cgcgcttgat gtccagggtg
     8041 tgggcgtgct cgccctcctt ctcgaggacg aacccgcgga agaacccgac cggcggctcg
     8101 ttggcgaccg ccgcaccggc caggtgggag aggaatcgct tcgacgacgg cgcctgcgag
     8161 cgcacgaggt cgtccagctc ggcatacagc tgcgcgtccc cggcgaccgg gcgcatgtcg
     8221 aagaagatgc tcgcgcgcag gatggcgtcc gggaccggcg cattcagcca ctcgccgaag
     8281 acgtgtttcc actcgcgcag ggtgatgcgc cacttcgggt tcgtcgccat gacgtcgccg
     8341 gggcagcgcg ggtagccggc ttcgacgagc cagcccgtca cgagttccgc gaactgggcg
     8401 aagtagtcca ggtctgccgg ctgggcggcg tcgtcgatga tcagcgcatt gtcctgatcg
     8461 gccgcgagtg cctgctccat gcgggcgcgc gagccgagcg tgacccagca ataggggacc
     8521 ggcggggcgc ccaggcgttc ctcggccagg gcgatgagcc ggcgctcgac cgcgtcgccg
     8581 actgaggtga cgatccggcc catgtccgcg gcgctggcgt cttgtgccac aagggattcc
     8641 acgacccgcg gcaagcgagc cgccgcggtg gccacgccgg cggagtcctt ctgcttggcg
     8701 atgtccccgg cgatatagac cgggttggcc tgctccaggc gcatcaggtc ggtggtcgtg
     8761 accacgccga tggcccggtc gccgtccatc agcggcaggt ggtggatatt gcgcgcgacc
     8821 atctcgagca tcgcctcgaa cgccaagccg tccgcgcggg cggtgaccgg gttggcggac
     8881 atgacgtcgg cgagcggtgt ggtgccgtcc cggccggcgg ccagcacccg gttgcgcagg
     8941 tcccggtcgg tgatgatgcc cgccagccgt tcgccgtcca tcacgagcag ggaggagacg
     9001 ccggcgtcgg acatcacctg cgccgcctca cgcacattcg cgcgcaccga cacggtgatg
     9061 ggtgccttgc gcgcgagttc ggacatccgc gtcttgagga tcgcgctccc cgaggcgctc
     9121 gcctgcttgg ccgcgaccgc cccggccatc cggcgggtgc gctgggcgtc gaagaactcg
     9181 ccgaactcgc gatggtcgtt gcacaactcg gcgaaggtcg ccgcccccat cgcgagggtg
     9241 agggtgtcct cgatggcgac gacgctgaac gtcgacgggt tgccgccgag gagagtggtg
     9301 gagccgaagc agtcgccgac ctcgccgcgg tcgacgagcg tcccgatggc atcgcgcatg
     9361 tccacggccc cggaacgcac gacatacagg tgcacgctgt cgcgtccggg cgtcatgatc
     9421 gccgtcccgc ggcggtggta ctcgaccgtc agccggggca ccagccgggt cagcgcctcc
     9481 tgcggaagat cggcaaaggg ttcctgggcc gccaggaagt cccgaatctc cgccaactcg
     9541 acatcgagtg ccatggggcc aatcgtgccg tgtgcgctgt tggtgcaccg aggagggtat
     9601 gcggcgcggt cgccggatgg tgcccgctgc gcgccaagtg gcgcgagcca ggagttgtga
     9661 gtgagtgctc actcacttat catcgtcggg tgaccccgcg cgcgccggct ctggcccccg
     9721 acgatcggcg ggccgccatc gtcgcggcca cgctgcccct ggtgctcgcc gagggccggg
     9781 cggtgaccac ccggcagatc gccgaggccg ccggtgtggc cgaaggcacg atcttccggg
     9841 tcttcgcgac caaggacgac gtcatcgacg ccgtcatcga ggcggccttg gacatggagc
     9901 cgttcatcgc cgcgctggag gcactgccat cgccggcgag tctggacgag gccgtgcact
     9961 ggctcgccca ggcgcagctc gagcggttcg cccaggtctt ctcgctgatg accgtgctcg
    10021 gcatccacgg cccgccgaaa caccaaccgc gccccgagtg gatcgcccgc ctcgcggccg
    10081 cgcaaatgga ggtgctggcc ccctttgccg aggagttgac cctccccccg cgtgaggtgg
    10141 tgcgctatat ccggctgctc gcgttcgccg gaggcaactc gcacatcacc gacggcgaac
    10201 tgctcacggc cgccggcatc acgaccctcg tcctcgacgg ctcccgaaag aagggctgac
    10261 ccacatgctg tggagactga tccgcaccca cctgggtccg tatgccggtg cggtcgccgc
    10321 catcctcacc ttgcagctgg tctcgaccgc gaccacgctc tatcttcctt cgctcaacgg
    10381 ccagatcatc gatgacggtg tggcactagg ggataccggc gagatcatgc gcctcggcgc
    10441 gatcatgctc gccgtctcgc tcgtcaacat cctgtccacg gtcgccatcg cgcgacaggc
    10501 atcggccacc tcggcgggga tggccagcga tctgcgtcgg gaggtcttcg gtcgcgtgac
    10561 gagcttctcg gcccaggagg tctcgcgctt cggcgccccg acactgatct cccgcaacac
    10621 caacgacgtc cagcaggttc agttggtcgt caacatgggc ctgatgttca tgatgtcgat
    10681 tccgatcatg atgatcggcg gcatcttcat ggcgctgcgc gaggacgtcg ggctgtcctg
    10741 gctcgtcgcg gtctcggtgc ccctgctcgg catcatcgtc ggctccatcg tcgtgaagat
    10801 gctgccgtgg ttccggcgga tgcaggagag cgtcgacgcc gtgaaccgca ttctgcgcga
    10861 acagatcacc ggcatccggg tggtccgtgc cttcgtccgc gaagacgtcg aggagaaacg
    10921 cttcgacgac gcgaacacca cctacaccgg cacggccgtc gccgtcggca aactcttctc
    10981 ctccgtcttc ccgctcgtga tgttcgtctt caacctggcg tcggtggcgg tgtggtggtt
    11041 cggtgccaag cgggtggacg ccggccagat ggaggtcggc gccgtcacgg cgttcatgca
    11101 gtacctcatg cagatcctca tgtcggtgat gttcgcgacg atgatgtcga tgatgatccc
    11161 gcgggcgtcc gtctcggcgg gacgcatcac cgaggtgctg cagactccgt cgagcgtgat
    11221 cgacacgccg gacgcgccgg gcctcgagga cgggcggctg ccggtccggt tcgacatggt
    11281 cgacttctcc tatccgggcg ccgaacagcc tgtgctgcac gacatcacct tccaggcgaa
    11341 cccgggccag acgacggcca tcatcggctc gaccggctcc ggcaagacga cactcgtcga
    11401 cctgattccc cgcctctacg acgtcaccga cggatcggtc cgcatcggtg accgggacgt
    11461 gcgggaggtc cctctcgagg acctctggcg gcacctgggt ctcgtcccgc agcggccctt
    11521 cctgttctcc ggcaccgtcg cgagcaacct gcgctacggc gacgaggagg ccaccgacga
    11581 gcaactgtgg gaggccctgc gcatcgccca ggcggaggac ttcgtctcgg ccatgcccgg
    11641 cggcctggac gccccgatca gccagggcgg cacgaatgtc agtggcggac aacgccaacg
    11701 gctcgccatc gcccgcgccc tcgtgcgccg gccggcggtc tatctcttcg acgacgcctt
    11761 ctccgccctc gacgtcgcaa ccgacgcacg cctacgcgcg gcgctgcgac cgcatacccg
    11821 cgacgcaacc gtgatcatcg tcgcccagcg cgtgtcctcg atcatgcacg ccgaccagat
    11881 catcgtcctc gacgacggcc gcgtcgtcgg tatcggcggg cacgagcagc tgctcgcctc
    11941 ctgcccgact tacgccgaaa tcgttgcctc ccaggcactc accgaggagg cagcatagcc
    12001 atggctgacc gcatactcac cgaagaggag aaggccgcgg agcagcgtcg cggcgccgct
    12061 gggtccgcgc agcgcagcgg gcccccgcac atgcggatgg gccagcccgg tgagaagtcc
    12121 cggaacttcg ggccgtcggc gcgccggctg atcggcctca tgggccgcga caagccgcta
    12181 ctgtggctcg ccctggcctt cgcgctcgtg tccgtcatcc tcaacgcgct cggccccaag
    12241 atcctcggcc gcgccaccga ctacgtcttc tccggcgccg tgggccgggt gctcccggac
    12301 ggcatgacgg tcgagcaggc cgtggcgttt ctgcgcgcgc agggcaagga tcaggtggcc
    12361 gacatggtgt cgggcatgaa gtacctcgtc ccggggcagg gtgtcgattt cacggccctc
    12421 ggccggatcc tcttgatcgt gctcgccctg tatgccgtgg cgagcctgct ttcgtgggtg
    12481 caggcccgga tcatcgtgcg tgtcgtcaac ggcgcggtct accggctccg gcgggacgtc
    12541 gaggagaagc tgagccggct gcccttgtcc tacttcgatt ctcagccgcg cggtgagttg
    12601 ctctcgcggg tgacgaatga cgtcgacaac atcggccagt cgctgcagca gaccctcagc
    12661 cagctcgtga ccagctttct gacggtcatg gccatgctcg tcatgatgct ctgggtctcc
    12721 ccgctgctgg ccatcatcgc gctggtgacg atcccggtct ccatcggtat caccgccgtc
    12781 atcggcaagc gcagccaggc gcacttcgtg cagcagtgga agtccaccgg tgaggtcaac
    12841 acgatcgtcg aggagacctt caccggccac ggcctggtca aggtcttcaa ccgtggcgcc
    12901 caggcgagcg ccgacttcgg cgtcaagaac gaggagttgc gcaaggccac tttcggggcg
    12961 cagttcgtct ccggcctgat catgccgatg atgatgttcg tcgggaacct caactacgtc
    13021 atcatcgccg tcgtcggcgg gctccgcgta gcccacgggc agatgagcat cggtgacgtc
    13081 caggcgttca tccagtactc ccgccagttc acccagccgc tgacgcaggt ggcctcaatg
    13141 gccaacctca tgcagagcgg tgtcgcctct gccgagcggg tcttcgaggt gctcgacgcg
    13201 ccggaacaga tcgcggacgc cgcggacgcc gtccacgtcg aggacccgca cggccgggtc
    13261 gcgttcgagg acgtctcctt ctcctacacc cccgaccgcc cgctcatcga gcacctcgat
    13321 ctcgtggtcg agccggggca gacggtggcg atcgtcggcc cgaccggtgc cggcaagacc
    13381 acgctggtca acctgatcat gcgcttctac gagttgaacg gcgggcggat cacgctggac
    13441 ggcatcgata tcgcgtcgat gcgccgcgcc gacctgcgtg gtgagatcgg catggtcctg
    13501 caggacacct ggctcttcga gggcacgatt cgcgagaaca tcgcctacgg caaacccggc
    13561 gcctccgacg ccgaggttcg ggcggccgcc gaggcgactt tcgtcgaccg gttcgtgcac
    13621 tcgctgcctg atggctatga gaccaagctc gactccgagg cgggcaatat cagcgccggg
    13681 gagaagcagc tcctgacgat cgcccgggcg ttcatctccg acccggccct gctgatcctc
    13741 gacgaggcca cctcctcggt cgacacccgc accgagctcc tggtccagca cgccatgcag
    13801 gccctgcgcc aggaccgcac gagcttcgtc atcgcccacc ggctctcgac gatccgcgac
    13861 gccgacctga tcctcgtcat ggaggacggc cgcattgtcg agcagggatc gcacgccgag
    13921 ctcctcgctg ccggcggcgc cttcgcccgc ctgtatgccg cccagttcgc cggcgctccc
    13981 gacgccgagg acgcggctgc gcccatcgcc tgacggacgc ttgctcgcgt ccggttgcgc
    14041 ctgcgtcatg cgcttcgtca cgtggcggaa acagcggccg ggacaaggtc cctcgcgccc
    14101 cggatcgggc ccaggcaccg agacgcgcct ggtcgagacc gtcgcgcgtc acgctgctcg
    14161 ggtggcagag gcctatctgc tgtcgctcga tgtccagggg gctcgcgaac ggctggtgtc
    14221 agcgcgtgag gaggagcgcg tgcgcctgcg caatgacctg catgacgggc tcgggccgct
    14281 gctggtcggc gcccggatgc aactggcggc cgccgctcgg gcgggcagcg acgctcgccg
    14341 tgcggatctg gtcgcaggtg cctcggctga cctcggcacg gcgagcacct cggtgcgcga
    14401 actcatcgac ggcctgcgtc ccgcggccct cgacggcggc ctcgtcacgg ctttggagga
    14461 agccgcccac gggctccttc cggactgcga ggtcgttctc gacgcgccgg ccgagctggc
    14521 gcccatgccg ccggccgtcg agatt
//
LOCUS       DAOPJG010000072        14538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4356_length_14538_cov_7.541186, whole genome shotgun sequence.
ACCESSION   DAOPJG010000072 DAOPJG010000000
VERSION     DAOPJG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 14538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14538
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4356_length_14538_cov_7.541186"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKU75_08300"
     CDS             complement(<1..279)
                     /locus_tag="PKU75_08300"
                     /inference="COORDINATES: protein motif:HMM:NF020466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcI/YcgG family protein"
                     /protein_id="HPF80915.1"
                     /translation="MTTVERTWPPLNEYLRDIARESLADAGEDAISDAVARMIAHPEY
                     PCLGARSVFRRDAARIVVLDSMADPDAVAQLAVHLEAFSNANRDPEDFV"
     gene            451..1182
                     /locus_tag="PKU75_08305"
     CDS             451..1182
                     /locus_tag="PKU75_08305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF80916.1"
                     /translation="MTSSDYWDEATAQRYDVACADMFAPDVLAPAVEFLATRAGEGRA
                     LEFAIGTGRVGIALRESGVDVVGIELSQPMVDVLHRKEPDLPVVVGDMATTRVEGEFS
                     LVYLVFNTIANLRTQAEQVECFRNAARHLAPGGRFVIELWVPGIRRFPPGSAAVPFDV
                     SPGHVGLDTYDLATQQGTSHHYTRQEDGTFRYGETNFRFIWPAECDLMAQLAGLELET
                     RVADWDGTPFTGDSPSHVSVWRKHP"
     gene            complement(1152..1481)
                     /locus_tag="PKU75_08310"
     CDS             complement(1152..1481)
                     /locus_tag="PKU75_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HPF80917.1"
                     /translation="MSAFLIVNIAMVLLWLVTTGPSGTSWPVYLLIAWGIGLTFHVLS
                     MRDHDPTAEEIESEARRIAERARGANRRARRTPPEPPHAPLGIRSGVGRRTPPPRRQG
                     CLRQTET"
     gene            1482..2066
                     /locus_tag="PKU75_08315"
     CDS             1482..2066
                     /locus_tag="PKU75_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80918.1"
                     /translation="MPAQGPPLLQILARHPAKAPRPTPARRGPGVATGVVLSAARASY
                     AEFYQPHDARGPDDAALLLDAAGNGDPPARAAITTRLLADGAQATDETIPKVDAVEIM
                     LGHNRHDFMVEAPVLRRLFDAGVPVNRRVLQQGPVAAAGQGRGGHSRAARPDLKLDVE
                     GFGKGRPVAPNSQGGEDNPEGRALNRRVEIRYEG"
     gene            complement(2076..3389)
                     /locus_tag="PKU75_08320"
     CDS             complement(2076..3389)
                     /locus_tag="PKU75_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPF80919.1"
                     /translation="MADLLYLVAGLALLLAVILPRLLQHVWLSPPLVLVLVGLGLGAT
                     PIADRVRFDPVKNGPIVEHVTELTVLIALMGVGLAIDRPIDLRSLASIRRWSPAWRLL
                     AVTMPLCIAGVALLGWAAGIAPAAALLLGAVLAPTDPVLASDVQVGAPTDPDAVESIE
                     EIDEHDEVRFALSSEAGLNDGLAFPFVYAAILLATTSALGWGDLAHWVSWYLLGKIAV
                     GVLAGLVVGWGLGKVAFGARSEPLRLAEHGESLLALAALLTAYGASEVCGGYGFLSVF
                     TCALSVRATERHHEYHRAMHEVVERLERLLTLLVLLVLGVAMSRGLLAHLDWRAAGLA
                     LALVFVVRPAAGMAALAGTRSPSGGRLTGRERLAIAFFGVRGVGSVYYLAYAVGHADF
                     AEARWLWSAVAFTIVLSVIVHGVLATPVMRHLEGRRAARGRRALD"
     gene            complement(3457..4608)
                     /locus_tag="PKU75_08325"
     CDS             complement(3457..4608)
                     /locus_tag="PKU75_08325"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HPF80920.1"
                     /translation="MRTVPTIFITLALGATTTVAPGVATAAVAPVADSAAMGMGNVYA
                     LTNDPMDNAVVAYKRDSMGMLMMSGTYLTGGKGASLHGAVVDRTASQGALAADRRHGK
                     LYAVNPGSNTLTVFSMTNGMLKRTQTVSTHGTFPVSVAVSPDGMRVFVLNARRGGSIQ
                     GYQLVDGRLVAKMSWNRKLMLDTMSGTDMEFVSTPGQVGFSPDGRNLIVTTKASSHSI
                     LVWPMTKDGAFRTEPTVNTIAGAVPFAFDFDMHGRLNVANAGTGTVGTYGIRSGGMLM
                     RYGQTPTKGEAVCWLAVHDDLIVSSNTGSNTVTSLRSRAAAPVRLGVMKTGAAPVDST
                     FSRDGMYFYVQTGGTNMIETYRVSATGVLKKMDADKLPGPIGSEGIVAW"
     gene            complement(4739..5635)
                     /locus_tag="PKU75_08330"
     CDS             complement(4739..5635)
                     /locus_tag="PKU75_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPF80921.1"
                     /translation="MVDPDPDPVTAASPPATLVHRWSLTPDGMPWRGFMSSVWPVRGP
                     EGQAWAVKMLDTHHTPAGEGAALETWAGDAWCASRVVRLIDRVNDIADIPEALLLERL
                     DAGRPLQDHPDIDAADAEIAGALAALGAVEAPAGVRHTTVELERIAGAIREHWAEAPV
                     LPVRAVSAALATLDAMTEDPTVAADRLLCHGDLHYNNVLRDLDDTRWVVIDPIPSAGV
                     REIEVIAPLRNRWEDTVASGDPDRFLARRLDRLSEAAGLHRTRARALAQAVAVDNLLW
                     LLPRDPDSMFVPPYSVLAGWVD"
     gene            complement(5738..8290)
                     /locus_tag="PKU75_08335"
     CDS             complement(5738..8290)
                     /locus_tag="PKU75_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPF80922.1"
                     /translation="MIHAHRLRERFGEQPELDPAELQATLGLASAAARWDVAAIVANT
                     LAIAHRVMARTAEATEAVESALTYARHAASPPEEARALMTRAMIRLGQGDMAGASADL
                     TTARSLVDIDPEQLVAGGVTAFHSGDLALAESMTREALSHNTISDNARLLALNNLGSM
                     LMTRQPVEAVGFIREGLALVSPANTVLRAVLECNLGVALAESGAVPEALAAFDRLEEL
                     GRAHDLGEYQAEFDMEVSGLLGRLRLLPEARASAARGLNRLAGPGAALTRADSLVTAA
                     RLAIADGDLDAGRRLLSDAKALYEAQDRPVGVAITSVHALILDGSGDHAAMAGYAEQL
                     AQSGLSRDAAWAWSQAASAAGEGRESAAAEDYLRRVVASDNADATLKAEALARIALAH
                     SDFATARAHAVTALGIIDGRAALADAPDLRHRIATERATFELLLRDACVADSAAAQLD
                     SLLRHRPPAQQPLAPGTAVDDQRAQWRDLSRRVESAQEDPFTLATLGADLAAVERRLR
                     ATEWTREGGSGGRAPFGLADLAAVAGCPLLVVARIGRDAVAFHHDDDRTERVVIGAWD
                     QVLADMGQLTRGCARLATGGASDAAYAAVRSLAMDLDATLGAILPEEAGRELLVVLDR
                     GLDSAPLTGLPRIWERAARFASLAVTAPVPQQPALPARPRVVVAEGPRLQYAADEAAE
                     VAQGWGDDAPLTTGTGVSEMIGEADIAHFAAHATLRWDNPLQSSIHLHDGPLALTELI
                     RLARVDAADRPRMRLLYLSACSLASAPSDAVMVGAVPMLAERVAAEVIASSIPLPDEA
                     APAVARAVYAAIRSGHSAAAGLAQARAALGEDPERTGVRAALTCLAAYVAAP"
     gene            8366..9688
                     /locus_tag="PKU75_08340"
     CDS             8366..9688
                     /locus_tag="PKU75_08340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HPF80923.1"
                     /translation="MADSGTFVPDGFATEDPRDIGSRFDELRGAALRDPKATSELLER
                     YRTFSRDESDPAGPGATIELVSTVDRLVVAGRLHVVTEHEERLEAALRQHHAGLKVIE
                     RDERIGVTLAGTDLDLDTLGRLVHDLRTTPSGEEPLGVGFDDVTYEAVVIAKPITPQG
                     AGVDVIRVKHQSSRPHGTGRGAGVTVGVIDGGFAADDAPPRTDGWFDNVKPPADGSPK
                     LNQADPALLDPGAGHGTFVTGIIAAHAPDAMIVQYRATDSWGFGSAWRLKDQLLQAVA
                     DGCQVINISLGFEDTTLIGSPGISAALHTVPDDVLVVAAAGNSGSSTPMLPASHKATV
                     GVGGLDLDHDPVGWSNRGPWVDFSALAVPVLSTFVVDPADPGGDPNPFGYWAGTSFAA
                     PKVSGELAVLLGAGRTPATALTELRARGIRRAPSPDFGTLLDLPDQFR"
     gene            9946..10542
                     /locus_tag="PKU75_08345"
     CDS             9946..10542
                     /locus_tag="PKU75_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF80924.1"
                     /translation="MTTGNRAASGRPVEDDVSDLVIGAREGDQIAWGHLVDRYARLVY
                     AVALRCRLTHADAADVSQTVWLRLAENLHRINDPSRVGAWLVTTTKRECLALIKARSR
                     YEPMDVSTMTLVDPSAGPDEIIARAESITEVAAAFALLTERCRELLRRLVIESESYQQ
                     IAADLEMPIGSIGPTRIRCLSLLRRHLEEAPTHEKQPY"
     gene            10523..10996
                     /locus_tag="PKU75_08350"
     CDS             10523..10996
                     /locus_tag="PKU75_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80925.1"
                     /translation="MRNNPIDFASDLTPAEQTLIAHLAALCRAEQQMPPDVALAATAL
                     HDWLDVDGQLAELAADPVLVRSDDAAFVFHANDIRIRIEVAPAGYRRRRVTVHTLCEA
                     GAPAPEEMAVQFPDGATQAIPLDRFGDHVGDLPAGTMRLMAVGASGGITTPWFTI"
     gene            11079..11540
                     /locus_tag="PKU75_08355"
     CDS             11079..11540
                     /locus_tag="PKU75_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80926.1"
                     /translation="MVTGDNTLLPADRALLAQLRELTAAEAEMPDDVALAAKAAQGWG
                     NIDAELAALVQEPVLTRGDGTAYSFAVAGREIRVEVEPVGYRRRRLNVVLREAGADPA
                     GVDVRVQRGDGEELAVATDHFGERSVEVGLGPVRVLAGFAAGPVTTGWFTI"
     gene            complement(11598..12083)
                     /locus_tag="PKU75_08360"
     CDS             complement(11598..12083)
                     /locus_tag="PKU75_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPF80927.1"
                     /translation="MPDPIAQARQLWSDHGWSDAALGMTAVTSLMRAQAIALKRVETT
                     LKPFDITFARYEVLMLLTFSQRGSLPMSLIGERLQVHQSSVTNAVQRLEQAGLVNRSV
                     RPDDRRAYVVTLTSRGRTLAERATAALNASVFADPGLPPARAEALVDILADLRHTAGD
                     F"
     gene            12174..>14538
                     /locus_tag="PKU75_08365"
     CDS             12174..>14538
                     /locus_tag="PKU75_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPF80928.1"
                     /translation="MSAPKPALHVPSNPVRFVTAASLFDGHDAAINIMRRLLQSQGAE
                     VVHLGHDRSVAEIVTAAIQEDVQGVAISSYQGGHVEYFTYLVEELKARGAGHVKVYGG
                     GGGVIVPEEIAQLASVGAHIFSPQDGQRLGLPGMINTLIAEADTDLATGADLDGLLAG
                     EERALARALTVLESGTDEAFARAVRDAAASRSVPVLGITGTGGSGKSSLTDELVRRLR
                     RDASDKLRVAVLAVDPTRRRGGGALLGDRIRMNSIEPGVVFFRSLATREAGAVLPSGI
                     DAMIAAAKAAAYDLVIVETPGIGQGDAAITEHVDVSLYVMTPEFGAASQLEKIDMLDY
                     ADVVAINKFERRGAEDARRDVAKQMVRNREAFGVPWEDMPVFGTSAARFDDDGVTALY
                     QALKGLLTDKGLKHFDGILPTPTTKTSTGLASVLPKGRERYLAEIAGTVRDYHATTAA
                     QSAAARTVQQVAATRDLLEHSAKDTDDVVDLLAEVEKDLTGESRAALAQWPATAASYD
                     GDEQVYVVRDKEIRTPLTRTTLSGTHVKRVALPRFTDHGELLRFLRSENLPGYFPFTA
                     GVFPFKRKEENPARMFAGEGDPARTNRRFKLLSAGQPATRLSTAFDSVTLYGRDPDPR
                     PDVYGKVGTSGVSVATLDDMKVLYGGFDLCSPTTSVSMTINGPAPSILAMFLNTAIDQ
                     QLDAFREEEGREPDAAEAEEIRARALSTVRGTVQADILKEDQGQNTCIFSTEFALRAM
                     ADIQEFFIAHQVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTYVE"
BASE COUNT         2300 a         5110 c         4991 g         2137 t
ORIGIN      
        1 gacgaagtcc tcggggtcgc ggttggcgtt cgagaaggcc tccaagtgga ctgccagctg
       61 cgcgaccgcg tcgggatccg ccatcgagtc caggacgacg atccgggcag catcgcgtcg
      121 gaacaccgat cgcgcgccga gacaagggta ttcgggatga gcgatcatgc gggccaccgc
      181 atccgagatc gcgtcctcgc cggcgtcggc gagcgactcg cgggcaatgt cgcgcaagta
      241 ctcgttcagc ggtggccagg ttcgttcgac tgttgtcacg cttcgaccct tgccaaatcg
      301 ggggtgccgg cgaaccatcc gcaggggtgc ccgcggacgc gtccacgcca acacctcgcc
      361 gtgcagcccg cgaccaggca ccgatcaggt tgcgtcggca tcacccgaaa accccggcat
      421 cccggcggtc aggctggcat cgtgtcagcc gtgacgagca gcgattactg ggatgaggcg
      481 acggcgcagc ggtatgacgt ggcgtgtgcc gacatgttcg cgcccgatgt cttggcaccg
      541 gccgtcgagt tcctggccac cagggctggt gagggccggg cgctggagtt cgcgattggg
      601 accgggagag tgggaatcgc gttgcgggag agcggggtcg acgtggtcgg catcgagttg
      661 tcgcaaccaa tggtcgatgt gctgcaccgc aaggagccgg atcttccggt cgtggtcggc
      721 gacatggcca caacgcgcgt cgagggggag ttcagcctcg tctatctggt cttcaacacg
      781 atcgccaatc tgcgcaccca ggccgaacag gtggagtgct tccgcaacgc ggctcgccac
      841 ctggctccgg gaggacggtt cgtcatcgag ctgtgggtcc ccggcatacg gcgattcccg
      901 ccgggctcgg ccgcggtgcc cttcgacgtc agccccggcc acgtcgggct ggacacctac
      961 gacttggcga cccaacaggg gacctcgcac cattacaccc gtcaggagga cggcaccttc
     1021 cgctacggcg agacgaactt ccgcttcatc tggccggccg aatgcgacct gatggcccag
     1081 ctggcggggc tggagttgga gacgcgcgtt gccgactggg acggcacgcc gttcaccggc
     1141 gacagcccca gtcacgtctc cgtctggcgt aagcacccct gacgccgggg cggtggcgtc
     1201 cgtcgtccga cgccggagcg gatcccgagt ggcgcgtgcg gaggttcagg gggcgtcctg
     1261 cgggcccggc ggttcgcacc gcgagcgcgc tcggcaatcc ggcgggcctc ggactcgatc
     1321 tcctccgccg tgggatcgtg gtcgcgcatg gagagcacgt ggaaggtcag gccgatcccc
     1381 caggcgatca ggagatagac cggccaggac gtccccgatg gtccggtggt gacgagccac
     1441 agcagcacca tggcgatatt gacgatgagg aaggcgctca cgtgccagcg caaggcccgc
     1501 cgcttctcca gattctcgcg cgccatcctg cgaaggcacc tcgtccgacc ccggcccgaa
     1561 ggggaccggg agtcgcgaca ggcgttgttc tctccgccgc ccgcgcgtcg tatgccgagt
     1621 tctatcagcc gcacgatgct cgtggcccgg atgacgctgc gctgctgctc gatgcggccg
     1681 gcaacggcga cccgccggcg cgggcggcga tcacgacacg actcctggcc gacggcgccc
     1741 aggcgaccga tgagacgatc ccgaaggtcg acgccgtcga gatcatgctc ggccacaatc
     1801 ggcacgactt catggtggag gcgcccgtgc tacgccgcct cttcgatgcc ggcgtgccgg
     1861 tcaaccggcg cgtcctacaa caaggacctg tcgcagcagc gggccaaggc cgtggcggcc
     1921 attcgcgagc ggcccgaccc gacctgaagc tcgacgtcga gggattcggc aagggccggc
     1981 cggtggcgcc gaattcccaa ggcggcgagg acaatcccga gggccgggcc ctgaaccgcc
     2041 gggtggagat ccgctacgag ggctagtgcc gtcggtcagt ccagggcgcg tcgcccccgg
     2101 gcggcccggc gcccctccag gtgccgcatc accggtgtcg ccaggacccc atgcacgatc
     2161 accgacagca cgatggtgaa cgcgacggcc gaccacagcc atctggcctc ggcgaagtcg
     2221 gcgtggccca cggcatacgc caggtagtag accgacccca ccccgcgcac gccgaagaag
     2281 gcgatggcca ggcgctcgcg gccggtcagg cgcccgccgg acggtgagcg ggtgccggcc
     2341 agggcggcca tacccgcggc ggggcgcacg acgaaaacca gggcaagcgc gagtcccgct
     2401 gcgcgccagt cgagatgggc gagcagtccc cggctcatcg cgacgccgag gacgagcagg
     2461 acgagcaggg tcagcagccg ttcgagccgt tccacgacct cgtgcatggc ccggtggtac
     2521 tcgtggtggc gctcggtcgc ccgcacgctc agcgcgcatg tgaagaccga cagaaagccg
     2581 tatccgccgc acacctcact cgcgccgtat gccgtgagca gggccgcgag cgccagcaga
     2641 ctctcgccgt gctcggccag gcgcagcggt tcgctgcgcg ccccgaaggc gaccttgccc
     2701 aggccccacc cgacgaccag tccggcgagc acgccgacag cgatcttgcc gaggagatac
     2761 cagctgaccc agtgcgcgag gtccccccac ccgagtgcgg acgtcgtggc gagcaggatc
     2821 gccgcataca cgaaagggaa tgccaggccg tcgttgagtc cggcctcgga gctgagggcg
     2881 aaacgcacct cgtcgtgctc gtcgatctcc tcgatcgact ccacggcgtc gggatcggtg
     2941 ggggcgccga cctggacgtc ggaggcgagg accggatcgg tcggtgccag gacggcgccc
     3001 agcagcagcg ccgccgccgg ggcgattccg gccgcccacc cgagcagcgc gacgccggcg
     3061 atgcacagcg gcatcgtgac ggcgagcagg cgccaggcgg gcgaccagcg ccggatcgag
     3121 gccaggctgc gcaggtcgat cgggcggtcg atggccaaac ccacgcccat gagggcgatc
     3181 aggaccgtca gttccgtgac gtgttcgacg atgggcccgt tcttgaccgg gtcgaatctg
     3241 acgcggtcgg cgatgggggt tgcccccagc ccgagcccga cgagcacgag gacgaggggc
     3301 ggggagagcc acacgtgctg caacaggcgg gggaggatga cggcgaggag cagcgccagg
     3361 ccagcgacga gatagagaag gtcggccatg gtggctccaa cctacggcct gaacgccctg
     3421 caaaggcccc acaatgaccg ggctggttca tctcactcac caggcgacga tgccctcact
     3481 gccgatcggt ccgggcagct tgtcggcatc catcttcttc agcaccccgg tggccgaaac
     3541 ccgatatgtc tcaatcatgt tcgtgccccc ggtctgcaca tagaagtaca ttccatcgcg
     3601 gctgaacgtc gagtcgacgg gcgccgcgcc ggtcttcatc actcccagcc ggacaggcgc
     3661 ggcggcgcgt gagcgcaggc tggtgaccgt attgctgccg gtgttggagg agacgatcag
     3721 gtcgtcgtgc accgcgagcc agcagacggc ttcccccttg gtcggcgtct ggccgtaacg
     3781 catgagcatg ccgccgctgc gaatgccgta ggtcccgacg gtgccggtgc cggcgttggc
     3841 gacattgagc cggccgtgca tgtcgaagtc gaaggcgaac ggcacggcac cggcaatcgt
     3901 gttgacggtc ggttcggtcc ggaaggcgcc gtccttcgtc atcggccaca cgaggatgct
     3961 gtggctcgag gccttggtcg tgacgatgag gttgcggccg tcggggctga atccgacctg
     4021 gccgggcgtg ctgacgaact ccatgtccgt gccggacatc gtgtccagca tgagcttgcg
     4081 gttccaactc atcttggcca cgagcctgcc gtccacgagc tggtaaccct ggatcgaacc
     4141 gccgcggcgg gcgttgagca cgaagacgcg catgccgtcg ggggagaccg cgacgctgac
     4201 ggggaaggtg ccgtgagtcg acaccgtctg cgtgcgcttc agcatcccgt tcgtcatcga
     4261 gaagacggtg agggtgttgc tgccgggatt gacggcatac agcttgccgt gccgccggtc
     4321 cgcagcgagc gctccctgcg aggccgtgcg gtcgaccacc gcaccgtgca gcgaggcgcc
     4381 cttgccgccg gtcaggtacg tgcccgacat catgagcatg cccatgctgt cgcgcttgta
     4441 ggcgaccacg gcattgtcca tggggtcgtt ggtgagcgcg tagacgttgc ccatccccat
     4501 cgccgcggaa tccgcgaccg gggccacggc agccgtggcg actccgggtg cgacggtcgt
     4561 ggtggcgccg agggcgaggg tgatgaaaat cgttgggaca gttcgcattg gttgctcctg
     4621 aaggtgagtg atttcgacct ttcaagaaaa ccgcggaccg tcgccgtggc ctaggggtgg
     4681 gcgccttaag gacttcttac ggatacgtcg ggccgcgtcg ggcaccggga ccgggaggtc
     4741 agtcgaccca gccggcgagc accgaatacg ggggcacgaa catgctgtcc gggtcgcgag
     4801 ggagcagcca cagcaggttg tcgacggcga cggcctgcgc gagggcgcgg gcacgcgtgc
     4861 ggtggagccc ggcagcctcg ctgagccggt cgagccggcg ggcgaggaag cggtcgggat
     4921 cgccactcgc caccgtgtcc tcccaccgat tgcgcagcgg cgcaatgact tcgatctcgc
     4981 ggacgcccgc gctcgggatc gggtcgatga cgacccaccg ggtgtcgtcg aggtcccgca
     5041 gcacgttgtt gtagtgcagg tccccgtggc acagcagccg atccgcagcg accgtcgggt
     5101 cctcggtcat cgcgtcgagg gttgcgagcg ccgcggagac cgcgcgcacg gggaggacgg
     5161 gcgcctcggc ccagtgctct cggatcgcgc cggcgatccg ctccagttcg acagtcgtat
     5221 gccgcacgcc cgccggcgct tccaccgcac cgagcgccgc gagggcgccg gcgatttcgg
     5281 cgtccgccgc gtcgatgtca gggtggtctt gcagcggccg gccggcgtcg agacgctcga
     5341 gaagcaaggc ctcggggatg tccgcgatgt cgttcacccg gtcgatgagc cgcacgacgc
     5401 gggacgcgca ccatgcgtca cccgcccacg tctccagggc cgcaccctct cccgccggcg
     5461 tgtgatgtgt gtccaacatc ttgaccgccc aggcctggcc ctcgggcccg cgcacgggcc
     5521 acacggacga catgaacccg cgccacggca taccgtcggg cgtgagggac cagcgatgaa
     5581 ccaatgtcgc gggtggggac gccgccgtga cggggtctgg gtccggatca accactctcc
     5641 caatctattc cgttcacctc cctgaagccg atgcatgacg catccgctca ccagcttcca
     5701 gcccggcgtc atccggaccg ctgggtgaac gccgtgatca gggtgcggcg acgtaggcgg
     5761 cgaggcaggt gagggccgcg cgcacgccgg tccgctcggg gtcttcgccg agggcggcgc
     5821 gcgcctgcgc caggccggcg gcagcgctgt ggccgctgcg gatcgcggca tacacagcgc
     5881 gggcgaccgc gggagcggcc tcgtccggca gcgggatgga ggaggcaatg acctcggccg
     5941 cgacgcgttc ggcgagcatc ggaaccgccc ccaccatcac ggcgtcgctc ggcgccgagg
     6001 cgagtgaaca cgcagacaga tagagcaggc gcatccgggg gcgatcggcc gcatcgacgc
     6061 gtgccaatcg gatcagttcc gtcagggcga gcggcccgtc gtgcaagtga atgctcgatt
     6121 gcagcgggtt gtcccaacgc aaggtggcgt gtgccgcaaa gtgcgcgatg tcggcctcgc
     6181 cgatcatctc gctcaccccc gtgccggtcg tgagcggcgc gtcgtcgccc cagccctgcg
     6241 cgacctccgc ggcctcgtcg gccgcgtatt gcaggcgcgg cccttcggcg accacgactc
     6301 gcggccgagc cggcagagcg ggctgctggg ggaccggcgc cgtcaccgcc agggatgcga
     6361 accgcgctgc gcgttcccag atgcgaggca gcccggtcag cggcgccgaa tcgagtcctc
     6421 gatcgaggac gacgagcaac tcgcgcccgg cctcctccgg cagaatcgcg ccgagggtgg
     6481 cgtccagatc cattgccaga gaacgaactg cagcgtatgc cgcgtcgctc gcgccgcccg
     6541 tcgccagccg ggcacagccg cgagtcagct gccccatgtc ggcgagcacc tggtcccagg
     6601 ccccgatgac gacgcgttcc gtgcggtcgt cgtcatgatg gaaagcgacc gcatcgcggc
     6661 cgatccgggc aacgaccaga agcggacagc cggcgacggc ggcgaggtcg gcgagcccga
     6721 acggggcccg gcccccgctg ccgccttcgc gggtccattc cgtcgcccgc aggcgacgct
     6781 ccacggccgc gagatccgcg ccgagcgtgg ccagggtgaa gggatcttcc tgggccgact
     6841 cgacacgccg cgacagatcg cgccattggg cccgttggtc gtcgaccgca gtccccggag
     6901 ccagcggctg ctgcgccgga gggcgatggc gcagcagcga atccaactga gcggcagcag
     6961 aatcagcgac gcacgcgtcc cgcaggagga gttcgaaggt ggcgcgttcc gtcgcgatcc
     7021 gatgccgcag gtcgggagcg tccgccaggg cggcccggcc gtcgatgatc cccagcgccg
     7081 tgacagcgtg ggctcgagct gtggcaaagt cactgtgagc caaggcgatc cgcgccaagg
     7141 cctcggcctt cagggtggcg tcggcattgt ccgatgccac gactcggcgc aaatagtcct
     7201 ctgccgcagc gctttccctg ccttccccgg cagcggaagc ggcctgtgac cacgcccagg
     7261 cagcatctcg agacaggccc gactgggcca gctgctcggc ataccctgcc atcgccgcgt
     7321 gatccccgga gccgtccagg atcagcgcgt gcaccgacgt gatggcgacc ccgacgggac
     7381 gatcctgcgc ctcatacaac gccttcgcgt cgctcagcag ccgccgcccg gcgtcgaggt
     7441 caccgtcggc gatcgccagt cgcgcggctg tcaccagcga gtcggcccgc gtcaacgccg
     7501 ccccgggacc ggccagccgg ttcagccccc gggccgcaga cgcccgcgcc tccgggagca
     7561 gccgcagccg gccgagaagg cccgacacct ccatgtcgaa ttcggcctga tactccccga
     7621 gatcatgggc gcggcccagc tcctccagcc gatcgaaggc ggccagcgct tccggcacgg
     7681 caccgctctc ggccagcgcc acaccgaggt tgcactccag cacggcacgc aagacggtgt
     7741 tggcgggact cacgagcgcc aggccctccc ggatgaaccc gaccgcttcg acgggctgac
     7801 gcgtcatcag catgctgccg aggttgttca aggcgagcag ccgggcattg tcggagatgg
     7861 tgttgtgcga caacgcttct cgggtcatgg actcggccag tgcgaggtct ccgctgtgga
     7921 acgccgtgac cccaccggcg acgagttgct ccggatcgat gtccaccagt gaccgggccg
     7981 tggtcaggtc ggcactcgcc ccggccatgt ccccctgccc gaggcggatc atcgcccggg
     8041 tcatcagcgc ccgcgcctcc tccggcggtg acgccgcgtg ccgggcgtag gtcagcgcgg
     8101 attcgaccgc ctcggtcgcc tcggcggtcc gggccatcac gcggtgggcg atggccaagg
     8161 tgttggcgac gatcgccgcg acatcccacc gggccgccgc gctcgccaga ccgagcgtgg
     8221 cctgcaattc ggccggatcg agttccggtt gctcgccgaa acgttccctg agacggtgtg
     8281 catgtatcaa cagcccgcct ggcgactcat ctcctacggt catgcaaccc cttcggccgc
     8341 ttcgtcccta gcactggaga acacgatggc agattccggc acgttcgttc ccgatggttt
     8401 cgcgactgag gacccccgcg acatcggttc ccgcttcgac gagttgcgcg gcgccgcgct
     8461 ccgcgacccg aaggcaacat cggaactgct cgagcgctac cgcaccttct cccgagacga
     8521 gtccgaccct gccgggccgg gggcgaccat cgagctggtg tccaccgtcg atcgcctcgt
     8581 cgtggccggc cggctgcatg tcgtcaccga gcacgaggag cgcctggagg cggcgctgcg
     8641 gcagcaccat gcgggcctta aggtgatcga gcgcgacgaa cggatcgggg tgactctcgc
     8701 gggcaccgac ctcgacctgg acacgctcgg ccgtctggtg cacgacctgc gcactactcc
     8761 ctccggggag gagccgctcg gggtcggctt cgacgatgtc acctacgagg ccgtcgtcat
     8821 cgccaagccg atcacgccgc agggtgccgg cgtcgacgtc atccgcgtga agcatcagtc
     8881 cagccgtccg catggcaccg gccgtggcgc aggggtcacg gtcggtgtca tcgacggggg
     8941 cttcgccgcc gacgatgcgc cgccgcggac cgacgggtgg ttcgacaacg tcaagccgcc
     9001 ggctgacggc tccccgaagc tgaaccaggc cgacccggcc ctgctcgacc ccggcgccgg
     9061 gcacggcacc ttcgtcaccg gcatcatcgc ggcccacgcg ccggacgcga tgatcgtgca
     9121 atatcgcgcc accgactcgt ggggcttcgg cagcgcgtgg cgtctgaagg accagctcct
     9181 ccaggccgtc gcggacggat gtcaggtcat caacatctcc ctcggtttcg aggacaccac
     9241 cctcatcggc tccccgggga tctccgccgc cttgcacacc gtcccggacg atgttctcgt
     9301 cgtcgcggcc gcgggcaact ccggtagctc caccccgatg ctgcccgcgt cgcacaaggc
     9361 caccgtcggc gtgggcggac tcgacctcga ccacgaccca gtgggctggt ccaaccgggg
     9421 tccgtgggtc gacttctccg cgctcgccgt gccggtcctc agcaccttcg tcgtcgaccc
     9481 ggcagacccg ggcggtgacc ccaacccctt cggctactgg gccggtacct ccttcgccgc
     9541 acccaaggtg agcggagaac tcgccgtcct gctcggtgcg ggacgcaccc ccgcgacggc
     9601 cctgacggaa ctgcgagcac gcggcatacg acgcgctccc agcccggact tcggcaccct
     9661 cctcgacctg ccggaccagt tccgctgagg acttctcggc ggctgccgcc gcgcgcgttc
     9721 acctgctctc cgcacgcaag cgatgggaaa cctctagcgg tggacacctt gccgggtcga
     9781 caatgccggg gtcgcctact tttcaacaat ctgcacgcgt atcaccgggc acccctgcgc
     9841 ctctgtcttg acaagcagcc acaacggctg atcgtgaccg aacgcaggag gacccatggc
     9901 ttcacggcac acgcgcacag gtccgacccc gaagggcaga tcaccatgac caccggcaat
     9961 cgagcggcct cgggccggcc cgtcgaggac gacgtcagtg atctggtcat cggcgcacgc
    10021 gagggggatc agattgcctg ggggcatctg gtcgatcgct acgcccgact ggtgtatgcc
    10081 gtcgccctcc gctgccggtt gacgcacgcc gacgcggccg acgtctccca gaccgtatgg
    10141 ctgcggctcg ccgagaacct gcaccgcatc aacgatccgt cacgcgttgg tgcctggctc
    10201 gtcacgacga cgaagcggga gtgcctggct ttgatcaagg cacggtcacg ttacgagccc
    10261 atggacgtca gcaccatgac cttggtggac ccctccgccg gaccggacga gatcatcgcc
    10321 cgggccgaat ccatcaccga ggtcgcggcg gctttcgccc tcctgaccga gcgatgtcgt
    10381 gaactgctgc gccgcctggt catcgaatcg gagagttatc agcagatcgc cgccgacctg
    10441 gagatgccga tcggatcgat cgggcccacc cggatccgat gcctgagcct cctgcgtcgc
    10501 cacctcgagg aggccccgac tcatgagaaa caaccctatt gactttgcct cggatctcac
    10561 cccggccgag cagaccctga tcgctcatct cgccgccctc tgccgggccg agcagcaaat
    10621 gccgcccgac gtggcgctcg cggccacggc cctccacgac tggctggatg tcgacggcca
    10681 actggccgaa ctcgccgccg accccgtgct cgtccgcagc gacgatgcgg catttgtctt
    10741 ccacgccaac gatatccgga tccggatcga ggtcgcaccg gcgggatacc ggcggcgccg
    10801 ggtcactgtc cataccctct gcgaggcggg cgccccggct cccgaggaga tggcggtcca
    10861 gttccccgac ggggccacgc aggccatccc actcgaccgg ttcggcgacc acgtcggcga
    10921 cctgccggcc ggcacgatgc ggctgatggc cgtcggcgca tccggcggga tcaccacgcc
    10981 gtggttcacg atctgacggg tctcccatcc gtcatccggc gggcgtgggg cgtaggctcg
    11041 atgcaggtac tggtccacgc tcccggaaga acatgcccat ggtcaccggc gataacaccc
    11101 tcctgcccgc cgatcgcgcg ctgctggcgc aactgcggga gctcaccgct gccgaggccg
    11161 agatgcccga tgatgtggcg ctcgccgcca aggcggccca gggctggggg aacatcgacg
    11221 ccgaactggc ggccctcgtg caggaaccgg tgctcacgcg cggtgacggg acggcataca
    11281 gcttcgcggt cgcgggccgt gagatccgcg tcgaggtcga gccggtcggc tatcgccggc
    11341 gccggctgaa cgttgtcctg cgcgaggccg gggccgaccc ggccggcgtt gacgtgcggg
    11401 tgcagcgcgg cgacggcgag gagcttgccg tggctaccga ccacttcggc gagcgctccg
    11461 tcgaggtcgg gctcggcccg gtccgcgtgc tcgccggctt cgctgccggc cccgtgacga
    11521 ccggctggtt cacgatctga ccgctggccc cgcttacgtc aaacccggcc cccgctcggg
    11581 gcgaaccggc cggggggcta gaagtcgccg gcggtatgcc ggaggtcggc gaggatgtcg
    11641 acgagggctt cggcgcgggc cggggggagg ccggggtcgg cgaagacgct cgcgttcagg
    11701 gccgcggtgg cgcgctcggc cagcgtgcgg ccgcgggaag tgagggtgac gacatacgcc
    11761 cggcggtcgt ccgggcgcac cgagcggttg accagtccgg cctgctcgag ccgttggacg
    11821 gcattggtga cgctgctctg gtgcacctgc aggcgctcgc cgatcaggga catcgggagc
    11881 gacccgcgct gcgagaacgt caggagcatc agcacctcat accgggcgaa ggtgatgtcg
    11941 aagggtttga gggtggtctc gacccgcttc agggcgatgg cctgggcgcg catgagggag
    12001 gtgacggccg tcatgcccaa cgcggcgtcg gaccagccgt ggtcactcca cagctgacgc
    12061 gcctgcgcga tcgggtccgg catgagcaaa ccgtagcggc atttgcatgg acgacctcgt
    12121 aattacatgg atgtccatgt atcgtggttc tcacaccatg tcgagggagc cccatgagcg
    12181 cgccgaagcc tgccctgcac gtccccagca acccggtccg gttcgtcacg gctgcaagcc
    12241 tgttcgacgg gcacgacgcg gcgatcaaca tcatgcggcg gctgctgcag agccagggcg
    12301 cggaggtcgt ccacctcggg cacgaccggt cggtcgccga gatcgtcacc gccgccatcc
    12361 aggaggacgt ccagggcgtc gccatcagct cctatcaggg cgggcacgtc gagtacttca
    12421 cctatctcgt cgaagagctc aaggcgcgcg gcgccgggca cgtcaaggtg tatggcggtg
    12481 gtggcggcgt catcgtgccg gaggagatcg cgcagcttgc ctcggtcggt gcgcacatct
    12541 tctcccccca ggacgggcag cgcctcgggt tgccggggat gatcaacacg ctcatcgccg
    12601 aggccgacac cgacctggcc accggcgccg acctcgacgg tttgctcgcg ggggaggagc
    12661 gggcactggc ccgggcgctg accgtcctcg agtccggcac ggacgaggcg ttcgcgcggg
    12721 cggtgcgcga cgctgcagca tcccggtccg tccccgtcct cggtatcacc ggcaccggcg
    12781 gctccgggaa gtcttctctc accgacgaat tggttcgtcg cctccggcgc gacgccagcg
    12841 acaagctgcg ggtcgcggtg ctcgccgtcg accccacgcg aaggcgcggc ggcggtgcgc
    12901 tgctgggcga ccgcatacgc atgaactcca tcgagccagg cgtcgtcttc ttccgcagcc
    12961 tcgccactcg cgaggccggc gccgtcctgc ccagcggcat cgacgcgatg atcgcggcgg
    13021 ccaaggcggc ggcatacgac ctcgtcatcg tcgagacacc cggcatcggc cagggcgatg
    13081 cggcgatcac cgaacacgtc gacgtctcgc tctatgtcat gacgccggag ttcggcgcgg
    13141 ccagccagct cgagaagatc gacatgctcg actacgccga tgtcgtggcg atcaacaagt
    13201 tcgagcggcg cggcgccgag gacgcccgcc gcgatgtcgc caaacagatg gtccgcaacc
    13261 gcgaggcctt cggcgtgccg tgggaggaca tgcccgtctt cggcacctcc gccgcccgct
    13321 tcgacgacga cggcgtcacc gcgctctatc aggcgctcaa ggggttgttg accgacaagg
    13381 gcctgaagca cttcgacggc atcctgccca cgcccaccac caagacctcc accggtctgg
    13441 cctccgtgct gccgaagggg cgcgaacgtt atctggccga gatcgccggc accgtccgcg
    13501 actaccacgc gacgaccgcc gcccagtcgg cggccgcgcg gacggtgcag caggtcgctg
    13561 caacgcggga tctgttggag cacagtgcca aagacaccga cgacgtcgtc gacttgctcg
    13621 ccgaggtcga gaaggatctc accggtgagt cgcgtgcggc gctcgcgcag tggccggcga
    13681 ccgccgcgtc atacgacggc gacgagcagg tgtatgtggt gcgggacaag gagattcgca
    13741 ccccgctgac ccgcaccacc ctcagcggca cccacgtgaa gcgggtcgcg ctgccgcggt
    13801 tcacggacca cggcgagttg ctccgtttcc tgcgcagcga gaacctgccg ggttacttcc
    13861 ccttcaccgc cggcgtgttc ccgttcaagc ggaaggagga aaatccggcg agaatgtttg
    13921 cgggagaagg cgatccggcg cgcaccaacc ggcgcttcaa gctgctttcc gccggccagc
    13981 cggcgacccg gctctcgacc gcgttcgact cggtcaccct ctacggtcgc gaccccgacc
    14041 cgcgcccgga cgtctacggc aaggtcggca cctccggtgt ctccgttgcg acgctggacg
    14101 acatgaaggt cctttatggc ggcttcgacc tctgctcgcc gacgacgagc gtgtcgatga
    14161 cgatcaacgg cccggcgccg tcgatcctcg cgatgttcct caacaccgcc atcgaccagc
    14221 agctcgacgc cttccgcgag gaggagggcc gagagccgga tgccgctgag gcagaggaga
    14281 ttcgggcccg ggccctgtcc accgtccggg gcaccgtgca agccgacatc ctcaaggagg
    14341 accagggcca gaacacctgc atcttctcca ccgagttcgc gctgcgggcg atggcggaca
    14401 tccaggagtt cttcatcgcc caccaggtcc gcaacttcta ttcggtctcg atcagcggct
    14461 atcacatcgc cgaggccggg gcaaacccga tcagccagct cgccttcacc ctcgccaacg
    14521 gcttcaccta cgtcgagg
//
LOCUS       DAOPJG010000073        14464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4381_length_14464_cov_7.665487, whole genome shotgun sequence.
ACCESSION   DAOPJG010000073 DAOPJG010000000
VERSION     DAOPJG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 14464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14464
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4381_length_14464_cov_7.665487"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKU75_08370"
     CDS             complement(<1..374)
                     /locus_tag="PKU75_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80929.1"
                     /translation="MSLIHLRSATTSFVAVNDGDALPIVLHWGARLPDEVTEAELLAA
                     MRPVVPHSSTDEVMPRRLLPMPVDGWRLRPTLAGSYADGTGFAPQFRCGAVESSDTAL
                     RIVAVADGAALAVETQYTMHPEG"
     gene            complement(421..939)
                     /locus_tag="PKU75_08375"
     CDS             complement(421..939)
                     /locus_tag="PKU75_08375"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPF80930.1"
                     /translation="MAEKPLIDIRWDEDKRRKFIETHNDRLPTKRIIAQGILRNERGE
                     VLLCQLTYKQEWDLPGGIVDKFESPAHCVARECREELGVDLPVGELLTTSWLPPYRGW
                     DDAVAFIFDLGVAQADLEARMTLQRREISAVHWVGPADLDAHAAPYAARLIRSALAAD
                     RTAYLENGESRS"
     gene            complement(932..1561)
                     /locus_tag="PKU75_08380"
     CDS             complement(932..1561)
                     /locus_tag="PKU75_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80931.1"
                     /translation="MPESIEQFHARVLAAQGTAGGRFPLPEDGYTTWASFPFDGELTV
                     KPIGPLAAEERPREGDDPADCHCAPGATPSPDWPVRWRDALWQIKQAPPSGSPLILIL
                     EPREHGDLGDLSRERAAEYGVLSVLIAQGMESLPSVGRCQIGRYGDGGAHAHVWFIAR
                     PARMPEVRGTFNILWDDLLPPMPRDVLRENADAVLGHVFAAYGGDLLDG"
     gene            1643..2833
                     /locus_tag="PKU75_08385"
     CDS             1643..2833
                     /locus_tag="PKU75_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPF80932.1"
                     /translation="MDGWVWGVCGLIAGALLAGALVWARYAGRAAAVVTERDLLRERV
                     VDLEASLAEDSETAALLAPLRDALGRVEHQVGVLERDRVEQFGSLRAVMARVEGETQE
                     LGRATASLAGSLRSSSVRGAWGEVQLRRVLEVSGLLPRCDLDEQVSAISRHGRTVRPD
                     VVVRLPGDKVIVIDAKAPMDAFLDAQAEDLGDDERIGLLRDHAVRLQRHIDALAAKDY
                     WSAFTASPEMVVCFVPSDAMLAAALAARPGLHEHALARRVALVGPGSLLALLRTVAFT
                     WQQESVASSAGELLELGTELYRRLATLGGHTAKLGRSLTSSVEAYNAMVGALETRVLV
                     TARRMQQLGLVTDEIPEGAAIETGARPMTAQELIDAATAEDARPELAFDVNRPPKARD
                     AKAV"
     gene            2865..3173
                     /locus_tag="PKU75_08390"
     CDS             2865..3173
                     /locus_tag="PKU75_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80933.1"
                     /translation="MTTSLQVRDVPDDVSRVLKARAARAGKSLSEYVLGELVEMARRP
                     TLADVLDELESRPVTRLNVSAADLIRAGRDERDRRIAEALDRADRPAGAPTANGDDVL
                     "
     gene            3170..3577
                     /locus_tag="PKU75_08395"
     CDS             3170..3577
                     /locus_tag="PKU75_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPF80934.1"
                     /translation="MTDVVIDASALIELLLATEIGGAVGAELRRLECRVLHAPGHVVA
                     EAVQGLRGIAMGLADLSRPCERAATHLSGLPMRLWDTRPLIARMWQLRANISAYDAAY
                     PALAEVTALPLITTDRRLARAVLGSSRCEVLAL"
     gene            complement(3587..4624)
                     /locus_tag="PKU75_08400"
     CDS             complement(3587..4624)
                     /locus_tag="PKU75_08400"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPF80935.1"
                     /translation="MRSTPEPTENEPVKRVLLAAPRGYCAGVDRAVVTVEKALELYGP
                     PVYVRKQIVHNKHVVTTLEKRGAIFVDETEQVPEGATVVFSAHGVAPVVHEEAQALSL
                     KTIDATCPLVTKVHREAVRFANEDYDILLIGHEGHEEVIGTSGEAPEHITLVDGPEDV
                     ANVTVRDPEKVVWLSQTTLSVDETLETVRLLRERFPAMQDPPSDDICYATQNRQLAVK
                     QMAADCDLMIVVGSRNSSNSVRLVEVALEHGARAGHLVDYAEEIDESWLDGVATVGVT
                     SGASVPEVLVRGVLDFLGERGYADVRPVVAAEESLVFSLPNEIRRDLKAAGMSDKMTH
                     DGSFAASGSLH"
     gene            4669..5904
                     /gene="xseA"
                     /locus_tag="PKU75_08405"
     CDS             4669..5904
                     /gene="xseA"
                     /locus_tag="PKU75_08405"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPF80936.1"
                     /translation="MSTQPSALPERAAETTPERPWPVRLLSLKIGEYIDKMSVVWVEG
                     QVVQLDRRAGTAYLTLRDTDVDMSFNVAISTNALDAMPRPLGQGERVVVQAKPAFWGK
                     RGSLTLDARQIRPVGIGELLARLEYLKRTLAAEGLFTADRKKPLPFLPHTVGLITGRA
                     SAAERDVVENARRRWPGIPFEIRPVPVQGSAAVEEVCRALAELDDLAHVEVIVIARGG
                     GSVEDLLAFSNEALVRAVAGARTPVVSAIGHDIDTPILDLVADVRASTPTHAAGLVVP
                     DAAEMRAQLDLTTLRLRRAIGDRLQRERRRLVELTSRPVLTDRTAYVRSQRIEIATMR
                     RRGQLRIEAAITREQDRVEHLRRQARMLSPQATLDRGYALVTTPDGEVVTDQDQVDLD
                     DLLRVRVARGDFAVRATAS"
     gene            5924..6142
                     /locus_tag="PKU75_08410"
     CDS             5924..6142
                     /locus_tag="PKU75_08410"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPF80937.1"
                     /translation="MAADQTEFPDVAEFSYEQARDELVDVVARLEGGQVGLEESMTLW
                     RRGEALAAHCTAWLTQAEAAVAEQSQEG"
     gene            complement(6155..6751)
                     /locus_tag="PKU75_08415"
     CDS             complement(6155..6751)
                     /locus_tag="PKU75_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HPF80938.1"
                     /translation="MNEQVDVGDPATSGGPASQASMYARGSAANMVRSLLVIGGLMVL
                     LFFMVARVNSVSGPDVDISRVATPIVKEYGWPIEAPAGLPDGWRPSAVRFVPSTDKLR
                     TWHLGYISPDGHYIAIEQTKDATEAWITQQTNRAPEGGTLEAGGRTWQKYVREAKTQN
                     SLVAPGPGDLTTIITGDGTFEELTLFADHLKPYESPAT"
     gene            6888..7802
                     /locus_tag="PKU75_08420"
     CDS             6888..7802
                     /locus_tag="PKU75_08420"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPF80939.1"
                     /translation="MDGQFLVIGEALIDIVARHAENPVEHVGGSPANVAVGLARLDNR
                     VIFATCLGEDTRGDRIRKHLQARGVTVLDGGEEHPTSTAQANIDAGGAATYVFDLHWD
                     PGRIEIGPRVSHIHTGSIAAVLEPGGTAVVDALMRGRAQATISYDPNFRPSIMGDVAA
                     ATARIEEIIALSDVVKASSDDMALMYPDLSIEGVLKKWGDLGPAVTVATLAEAGVMYR
                     ITRTGEVSSRPAPPTHVVDTVGAGDSFMSGLISGLSTAGLIGGVDARGRLATAGPGQL
                     DPAIERGALCGAMTVSRAGAYAPSLDEL"
     gene            7834..9519
                     /locus_tag="PKU75_08425"
     CDS             7834..9519
                     /locus_tag="PKU75_08425"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPF80940.1"
                     /translation="MPDFAYEDMLPIGEDTTTYRLLTSDGVEAVTGPDGTEFLRVSDE
                     AMRLLAETAMHDIAHYLRPAHLAQLRKILDDPDASNNDKFVALDLLKNANISAGGVLP
                     MCQDTGTAIVMGKRGQRVLTTGPDEAALSHGVFDAYTRLNLRYSQMAPLTMWEEKNTG
                     SNLPAQIELYADTAPGHETTYKFLFMAKGGGSANKSFLFQETKAVLNPDRLMSFLDEK
                     LRGLGTAACPPYHLAIVIGGTSAEFALKTAKYASAKYLDNLPTEGSMTAHGFRDPELE
                     EQVLELTRQFGIGAQFGGKYFCHDVRVVRLPRHGASLPVAVAVSCSADRQCLAKITKD
                     GVFIEQLETDPAGYLPEQTHDSLADGEDAGDVVAIDLNRPMSEILAELTKHPVKTRLS
                     LTGPLVVARDIAHAKIKERLDAGEEMPAYLKDHPVYYAGPAKTPEGMPSGSFGPTTAG
                     RMDSYVDQFQAAGGSMVMLAKGNRSQQVTDACQAHGGFYLGSIGGPAARLAQDCIKSV
                     EVLEYPELGMEAVWKIEVEDFPAFIVVDDKGNDFFASVTKPVAFSIGKRPGLA"
     gene            9616..10227
                     /locus_tag="PKU75_08430"
     CDS             9616..10227
                     /locus_tag="PKU75_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPF80941.1"
                     /translation="MAGNARFMADERAHPHQDMDVRHRLAQKQAPFAVFFGCGDSRVA
                     AEIIFDQGLGDLFVVRTAGHVVDFSVLGSIEFGVGNLGSPLIVVLGHDSCGAVAAAIA
                     SARSGQMPKGYVRDIVERVTPSVFAVAQRGELTPESVEREHVRVTCELLYERSMIIQD
                     AVDAGSLAIVGCEYRLVDGAAHLVHVIGDVGDEVEVSDGDSRI"
     gene            complement(10236..11582)
                     /gene="glmM"
                     /locus_tag="PKU75_08435"
     CDS             complement(10236..11582)
                     /gene="glmM"
                     /locus_tag="PKU75_08435"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPF80942.1"
                     /translation="MARLFGTDGVRGLANKDITAELALGLSVAAAHVLGEPARAAGRK
                     PKAVVGRDPRASGEFLSAAVVAGLASAGVDVNDVGVLPTPAVAFLTADMDAHFGVMLS
                     ASHNAMPDNGIKFFAAGGHKLPDAVEDDIAERLHQEWTRPIGAEVGRVHTNRAGQARY
                     VAHLLAAVPNRLDGLTIVIDGAHGAASAVSPEVFRLAGADVIEIGTAPDGLNINDGYG
                     STHLEHLQAVVVDREADLGIAHDGDADRCLAVDHTGTVVDGDQIMAILALAMQQRGTL
                     KADTLVATVMSNLGLLQAMDRAGITVVKTGVGDRYVLEAMRENDFSLGGEQSGHVILL
                     DHGTTGDGVLTGLMLAARVAETGKPLAELASVMTRLPQVLINVKGVDASRVDTDPRLQ
                     SAVADEESALAGSGRVLLRKSGTEPLVRVMVEAATPAAAQAVAERLAGVVKESLGR"
     gene            complement(11794..>12340)
                     /locus_tag="PKU75_08440"
     CDS             complement(11794..>12340)
                     /locus_tag="PKU75_08440"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF80943.1"
                     /translation="HGAMSRRPQTEIAILGALSIGPMTGYELRAAITDVLGDFWHESF
                     GQIYPTLATLEESGSVRRATPGRTSGSRFEITSAGRAVLRERLAEPHTAPPPRNALLL
                     RLFFGSELPPGRAAELLRAEIDRATTALGRFAAIRAEVVAEPESEARTFRSLTLSYGE
                     HHARAHLTWAREALAALPQDV"
     assembly_gap    12341..12440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12601..13095)
                     /gene="rpsI"
                     /locus_tag="PKU75_08445"
     CDS             complement(12601..13095)
                     /gene="rpsI"
                     /locus_tag="PKU75_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPF80944.1"
                     /translation="MTDTTYDHDEDVELTEYTSESEGPVSDEPARKPTASAPGAGTGR
                     RKQAIARVRIVPGTGEWTINGRTLENYFPNKVHQQIVNEPLAVLELDGAYDVLVRVHG
                     GGPSGQAGAVRLGVARSLNGVDAELNRPALKKAGFLTRDARVPERKKAGLKKARKAPQ
                     YSKR"
     gene            complement(13135..13578)
                     /gene="rplM"
                     /locus_tag="PKU75_08450"
     CDS             complement(13135..13578)
                     /gene="rplM"
                     /locus_tag="PKU75_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPF80945.1"
                     /translation="MRTYTPKAAEITRQWHVIDATDVVLGRLASQTAALLRGKHKATF
                     APHVDTGDFVIIINADKVALTGAKLQQKRAYRHSGYPGGLKSETYAQLLDRDPAKAVE
                     KAVRGMLPKTTLGRQQLSKLKVYAGAEHPHTAQKPVPYTISQVAQ"
     gene            13818..14399
                     /locus_tag="PKU75_08455"
     CDS             13818..14399
                     /locus_tag="PKU75_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF80946.1"
                     /translation="MTDSASYIRLDSTSVKVLAHPLRSRLVGALRLHGPSTATALAKV
                     LSTNSGATSYHLRKLAEVGLVVDTGEGDGKSRLWAAASETTQYLSSDFSEDEDAATAL
                     GWLERDWLKHFTEKFGRWLDVRQGWPAVWRDRASMNDDTVLVTADQLRALHAEIDEVV
                     DRYRRVGQGNPDAKRIATYLVFYPVDMDKAPRA"
BASE COUNT         2204 a         4855 c         5046 g         2259 t
ORIGIN      
        1 ccctccgggt gcatcgtgta ctgggtctcg acggccagcg ccgcaccgtc tgccaccgcg
       61 acaatccgta gcgcggtgtc gctggactcg actgccccgc aacggaactg gggcgcgaag
      121 ccggtgccat cggcatacga cccggcgagc gtgggccgca ggcgccagcc gtccacgggc
      181 atgggcagca gccgccgcgg catcacctcg tcggtggagg aatgcgggac gaccggtcgc
      241 atggccgcga gcagctcggc ctcggtgacc tcgtccggca gccgggcacc ccagtgcagc
      301 acgatcggca gcgcgtcacc gtcgttcacg gcgacgaaag acgttgtcgc actgcgtaag
      361 tgaatcaggg acacgatggt ctcctccggg tcaacgggtc aggggtcagc ggttagtggg
      421 tcaggaccgg gattcgccgt tttcgaggta tgccgtgcgg tccgccgcga gggcagaccg
      481 gatgaggcgc gcggcatacg gcgcggcatg cgcgtcgagg tcggcgggcc cgacccagtg
      541 cactgcgctg atctcgcgcc gctgcagcgt cattctggcc tcgagatctg cctgagccac
      601 accgagatcg aagatgaagg cgacagcgtc gtcccagccg cgatagggcg gcagccagga
      661 cgtggtgagc aactctccga ccggcagatc gacgccgagc tcttcacggc actcccgcgc
      721 gacacagtgc gcaggcgatt cgaacttgtc gacgatgccg ccaggcagat cccactcctg
      781 tttgtaggtc agctggcaga gcaggacctc gccccgctcg ttgcgcagga tcccctgggc
      841 gatgatccgt ttcgtcggca accggtcgtt gtgagtctcg atgaacttgc gccgcttgtc
      901 ctcgtcccag cggatgtcga tgaggggctt ctcagccatc gagcaggtct cccccgtatg
      961 ccgcgaagac atgaccgagc accgcgtccg cgttctcgcg caacacatct cgcggcatgg
     1021 gcggcagcag gtcgtcccag aggatgttga aggtgccgcg cacttcgggc attcgagcgg
     1081 gacgtgcgat gaaccacacg tgggcgtgcg caccgccgtc gccgtagcgg ccgatctggc
     1141 accgcccaac gctcggcagg ctctccatgc cctgtgcgat gaggacgctg agcacgccgt
     1201 actccgccgc ccgttcgcga gagagatcgc cgaggtcgcc gtgttcgcgc ggctccagga
     1261 tgaggatgag cggcgagccg gagggcggtg cctgtttgat ctgccagagc gcgtcgcgcc
     1321 agcgaacggg ccagtcgggc gagggcgtcg cgccgggcgc gcaatggcag tcggccggat
     1381 cgtcgccctc ccggggccgc tcctcagcgg ccagcggacc gatcggtttg acggtcagct
     1441 cgccgtcgaa ggggaagctc gcccatgtcg tgtagccgtc ctccggtagc ggaaaccgcc
     1501 cgccggccgt cccctgggcc gccaggacgc gggcgtggaa ctgctcgatc gactccggca
     1561 cggtctcgag gctacctgcc tcgcgacagg gcgggagagg aactcggcga ggttgtcgga
     1621 cctcggtggc atggtgtgcg tcatggacgg atgggtctgg ggcgtatgcg gtctgatcgc
     1681 cggcgcactg ctggcgggcg cgttggtttg ggctcggtat gccggtcgcg cggccgccgt
     1741 ggtcaccgag cgcgatctgc tgcgcgagcg cgtggtcgac ctggaggcct cgctcgccga
     1801 ggactccgag acggcggcgc tgctggcgcc gttgcgggat gcgctgggcc gggtggagca
     1861 tcaggtcggg gtgctcgagc gcgaccgggt ggagcagttc ggctcgctgc gggcggtgat
     1921 ggcgcgggtc gagggcgaga cgcaggagct ggggcgagcg acggcctcgc tcgcgggctc
     1981 gttgcgctcc tcgagcgtgc gcggggcctg gggcgaagtg cagctgcgac gggtcttgga
     2041 ggtgtcgggg ctgctgccgc gctgcgatct cgacgagcag gtcagcgcca tctcacggca
     2101 cggccggacg gtccggcccg atgtggtcgt gcgcctgccc ggagacaagg tgatcgtcat
     2161 cgacgccaag gcgccgatgg atgccttcct cgacgctcag gccgaggatc tcggcgatga
     2221 cgagcggatc gggttgttgc gcgatcacgc ggtccggctg cagcggcaca tcgacgcgct
     2281 ggcggccaag gactactggt cggccttcac ggcgagtccc gagatggtgg tgtgcttcgt
     2341 cccgagcgac gccatgctcg cggccgcatt ggccgcgcgg cccgggttgc acgagcacgc
     2401 cctggcgcgc cgggtggcgt tggtcgggcc ggggtcattg ttggcgctgc tgcgcaccgt
     2461 cgccttcacc tggcagcagg agagtgtggc ttccagcgcc ggcgagttgc tggaactggg
     2521 caccgagctc tatcggcggc tggccaccct cggcggacac acggccaagc tgggtcgcag
     2581 cctgaccagc tcggtcgagg cctacaacgc catggtcggg gcgctcgaga cgcgggtact
     2641 ggtcacggcc cggcggatgc agcagctcgg cctggtgacc gacgagatcc ccgaaggggc
     2701 tgccatcgag acgggcgcgc ggccgatgac ggctcaagaa ctcatcgacg ccgccacggc
     2761 cgaagacgcc cgccccgagc tggcgttcga cgtcaaccgg ccgccgaaag ctcgggacgc
     2821 gaaagccgtt tgacatgcac attgcatgtg gccaggctgt aggggtgacg accagtcttc
     2881 aggtgcggga cgtccccgac gacgtgagcc gggtcctcaa ggcgcgtgcg gcgcgggccg
     2941 gcaagtcgct cagtgaatac gtcttggggg aactcgtcga aatggctcga cggccgacct
     3001 tggccgatgt gcttgacgag cttgaatcac ggcccgtgac gaggttgaac gtcagcgccg
     3061 ccgacctgat ccgggcgggg cgggacgaac gagaccgcag gatcgccgag gcgttggatc
     3121 gtgcggacag gccggccggc gcccccacgg ccaacgggga tgacgtccta tgaccgatgt
     3181 cgtgatcgac gcctccgcac tcatcgagct gctgctggcg acggagatcg gcggcgcggt
     3241 cggtgccgaa cttcgccgcc ttgaatgtcg cgtcttgcac gctcccgggc acgtcgtggc
     3301 ggaagcggtc cagggattgc gcggcatagc gatgggcctg gccgaccttt cccggccatg
     3361 cgagcgggcg gcgacgcacc tgagcggatt gcccatgagg ctgtgggaca cccgaccgtt
     3421 gattgcccgc atgtggcagc tccgggccaa catctccgcc tacgacgccg cgtacccagc
     3481 gttggccgag gtgactgccc tgcccctgat caccaccgat cgacgcctgg cgcgcgcagt
     3541 gctgggctcc agtcgatgcg aggttctcgc gctctgagcc gcccgttcag tgaagtgagc
     3601 cggaggcggc gaaggagccg tcgtgggtca tcttgtccga catgcccgcg gccttgaggt
     3661 cccggcggat ctcgttgggc agggagaaca ccaacgactc ctcggccgcg acgacggggc
     3721 gcacgtcggc atacccgcgc tcgccgagga aatcgagcac gccgcgcacg aggacctcgg
     3781 gcaccgacgc ccccgaagtg acacccaccg tggcgacgcc gtcgagccag gactcgtcga
     3841 tctcctccgc atagtcgacg agatggcccg cccgggcgcc gtgctcgagg gcgacctcga
     3901 cgaggcgcac ggagttggag gagttgcggg agccgacgac gatcatcaga tcgcagtccg
     3961 cggccatctg cttcaccgcc agctgacggt tctgcgtggc gtagcagatg tcatcgctcg
     4021 gggggtcctg catggccggg aagcgttcgc gcagcaaccg cacggtctcc agcgtctcgt
     4081 ccaccgagag ggtggtctgg gagagccaca cgaccttctc cgggtcgcgg acggtgacat
     4141 tcgcgacgtc ctcggggccg tcgacgagcg tgatgtgctc gggtgcctcg ccggaggtgc
     4201 cgatgacctc ctcgtggccc tcgtgcccga taagaaggat gtcgtagtcc tcattggcga
     4261 agcgcaccgc ctcgcggtgc accttggtca ccagcgggca ggtggcgtcg atggtcttga
     4321 ggctgagggc ctgcgcctcc tcgtggacca cgggcgcgac gccgtgcgcg gagaagacga
     4381 ccgttgcgcc ctcgggcacc tgctcggtct cgtcgacgaa gatcgcgcca cgcttctcca
     4441 aggtggtcac cacgtgcttg ttgtgcacga tctgcttgcg cacgtagacc ggcgggccat
     4501 agagctccag cgccttctcg accgtcacca cggcccgatc cacgccggcg cagtagccgc
     4561 gcggcgctgc caggagcacc cgcttgacgg gctcgttctc ggtcggctcg ggggtggaac
     4621 gcatatggca agtctaagtt gtcgggcctg tcccctaatc tctgccacgt gagcactcag
     4681 ccatcggcgc tgcccgaacg agccgccgag accactccgg agcggccctg gcccgtgcgt
     4741 ctgctgagcc tgaagatcgg cgaatacatc gacaagatgt cggtggtctg ggtcgaaggc
     4801 caagtggtcc agctcgaccg gcgggccggc acggcatacc tcacgctgcg cgacaccgat
     4861 gtcgacatgt ccttcaatgt cgcgatctcc accaacgccc tcgacgccat gccccgcccc
     4921 ctcggccagg gcgagcgcgt cgtcgtccag gccaagccgg ccttctgggg caagcgcggc
     4981 tccctcaccc tcgacgcccg ccagatccgc ccggtcggga tcggcgagtt gctcgcccgc
     5041 ctcgagtacc tcaaacgcac cctcgccgcc gagggcctgt tcaccgccga ccgcaagaag
     5101 ccgctgccct tcctgcccca cacggtcggg ctgatcaccg ggcgggcgag cgcggccgaa
     5161 cgggatgtgg tcgagaacgc ccgccgccgc tggccgggca tccccttcga gatccgcccg
     5221 gtgccggtgc agggcagcgc cgccgtggag gaggtatgcc gcgccctcgc cgagctcgac
     5281 gacctcgccc acgtcgaggt catcgtcatc gcccgtggcg gcggctcggt cgaggacctg
     5341 ctcgccttca gcaacgaggc gctggtgcgt gcggtggccg gcgcccgcac cccggtcgtc
     5401 agcgccatcg gccatgacat cgacaccccg atcctcgacc tcgtcgccga cgtccgcgcc
     5461 tccaccccca cccacgccgc cggcctcgtc gtgcccgacg ccgccgagat gcgcgctcaa
     5521 ctcgacctga cgacgctgcg cctgcgtcgc gccatcggcg accggctgca gcgcgagcgc
     5581 cggcgcctcg tcgagctgac gagccggccc gtgctgaccg accgcaccgc atacgtccgc
     5641 agtcagcgga tcgagatcgc caccatgcgc cggcgcggcc agctgcgcat cgaggcggcc
     5701 atcacccgcg agcaggaccg cgtggagcac ctgcggcggc aggcgcggat gctctccccg
     5761 caggcgacgc tcgaccgcgg ctacgccctg gtgaccaccc ccgacggcga ggtcgtcacc
     5821 gatcaggacc aggtcgacct cgacgacctg ctgcgcgtgc gagtggcgcg gggcgacttc
     5881 gcggtgcgcg cgacggcgag ctgacctcat acagttatcg accatggccg ccgaccagac
     5941 cgagttccct gacgtcgccg agttctccta tgagcaggcc cgcgacgaac tggtggatgt
     6001 cgtcgcccgc ctcgagggcg ggcaggtggg gctggaggag agtatgacgc tgtggcggcg
     6061 cggcgaggcc ctggccgcgc actgcacggc ctggctcacc caggccgagg cggcagtggc
     6121 cgagcagagc caggagggct gaggggcgcg agcgtcaggt cgccggcgac tcatacggtt
     6181 tgaggtgatc ggcgaagagg gtgagctcct cgaaggtgcc gtcgccggtg atgatcgtgg
     6241 tgaggtcgcc cgggcccggc gcgacgaggc tgttctgggt cttggcctcc cgcacgtact
     6301 tctgccaggt gcgcccgccc gcctcgagtg tcccgccctc gggggcacgg ttggtctgtt
     6361 gcgtgatcca ggcctcggtc gcgtccttgg tctgctcgat ggcgatgtag tgcccatcgg
     6421 gcgagatgta cccgagatgc cacgtgcgca gcttgtcggt cgaggggacg aaccggacgg
     6481 cgctcgggcg ccagccgtcg gggagcccgg ccggggcctc gatcggccag ccgtactcct
     6541 tgacgatggg ggtggccacc cgcgagatgt cgacatcggg gccggacacc gagttgaccc
     6601 gggccaccat gaagaagagc agcaccatca ggccaccgat gaccagcaaa gagcgcacca
     6661 tattggctgc cgagccccgg gcatacatgc tcgcctggga tgccggcccg cccgaggtcg
     6721 cggggtcgcc cacatccacc tgctcgttca tggccctatc gtcgcccaca tccgcaggtt
     6781 tgcgcgaaac cgtgttgtga aaggcgccgc tgacgcagcc gcggcgcggc gatagagtcg
     6841 accttggacg gaccaactga actgcgacga aggggcctgg gcgagccatg gacggacaat
     6901 tcctggtcat cggggaagcc ctgatcgaca tcgtcgcccg gcacgcggag aaccccgtcg
     6961 aacatgtcgg cggctcgccg gccaatgtcg ccgtcggcct cgcccgcctc gacaatcgcg
     7021 tcatcttcgc cacctgcctc ggcgaggaca cccgaggcga ccgcatccgc aaacacctgc
     7081 aggcccgcgg cgtcaccgtc ctcgacggcg gcgaagagca tcccacctct acggcccaag
     7141 ccaatatcga cgcgggtggt gccgcgacct acgtcttcga cctgcattgg gatccgggcc
     7201 gcatcgagat cggcccacga gtcagccaca tccacaccgg gtcgatcgcc gccgtcctcg
     7261 agccgggcgg cacggcggtc gtcgacgccc tcatgcgcgg gcgggcgcag gcgaccatct
     7321 cctacgaccc gaacttccgc ccctcgatca tgggcgatgt ggccgcggcg accgctcgca
     7381 tcgaggagat catcgcgctc agcgatgtcg tgaaggccag cagcgacgac atggccctga
     7441 tgtatcccga cctgtccatc gagggcgtgc tcaagaagtg gggagacctc ggcccggccg
     7501 tcaccgtggc caccctcgcc gaggccgggg tgatgtatcg gatcacgcgc acgggcgaag
     7561 tctcgagccg gcccgcgccg ccgacacatg tcgtggacac ggtcggggcc ggtgactcgt
     7621 tcatgtccgg gctgatctcc ggcctgagca ccgccggcct gatcggcggc gtcgacgccc
     7681 gaggccgtct ggccaccgcc ggcccgggcc aactcgatcc cgccatcgag cgcggcgccc
     7741 tctgcggggc gatgacggta tctcgggccg gcgcttacgc gccgagtctt gacgagctgt
     7801 gagttgaaca acgaaaggtg atgtgccaca accatgcccg atttcgcata tgaggacatg
     7861 ctcccgatcg gcgaggacac cacgacctat cggctgctca cgagcgacgg cgtcgaggcg
     7921 gtcaccggac cggacgggac cgagttcctc cgcgtctccg acgaggcaat gcggctgctg
     7981 gcggagacgg ccatgcacga catcgctcac tatctgcggc ccgcgcactt ggcccagctg
     8041 cgcaagatcc tcgatgaccc cgacgcgtcc aacaacgaca agttcgtggc cctcgacctg
     8101 ttgaagaacg ccaatatctc ggcgggcggg gtgctgccga tgtgccagga caccggcacg
     8161 gccatcgtca tgggcaagcg cgggcagcgc gtcctgacga ccggtcccga cgaagctgcg
     8221 ctgtcccacg gggttttcga cgcctacacg cggttgaacc tgcgctacag ccagatggcg
     8281 cccttgacga tgtgggagga gaagaacacc gggtccaacc tgcccgcaca gatcgagctc
     8341 tacgccgaca ccgcgccggg gcacgagacg acctacaagt tcctcttcat ggccaagggt
     8401 gggggctcgg ccaacaagtc cttcctgttc caggagacca aggccgtcct caatcccgat
     8461 cggctgatga gcttcctcga cgagaaactg cgcgggctcg gcacggccgc ctgcccgcca
     8521 taccacctcg ccatcgtcat cggcggcacc agtgccgaat tcgctttgaa gacagcgaaa
     8581 tacgcgagcg caaagtatct cgacaacctc cccaccgagg ggtcgatgac ggcgcacggc
     8641 ttccgggacc cggagttgga ggagcaggtt ctcgagctga cccggcagtt cggcatcggg
     8701 gcccagttcg gcggcaagta cttctgccac gatgtgcgag tcgtccgcct gccgcgccac
     8761 ggtgcctccc ttcccgtggc cgtcgccgtg tcgtgctcgg ccgatcgcca gtgcctggcc
     8821 aagatcacca aggacggggt gttcatcgag caactcgaga ccgacccggc cggctatctg
     8881 cccgagcaga cacacgactc gctcgccgac ggcgaagacg cgggtgatgt cgtggccatc
     8941 gacctcaacc ggccgatgag cgagatcctc gccgagctga cgaaacaccc ggtcaagact
     9001 cggctttcgc tcaccggtcc cctcgtcgtg gcacgcgata tcgcccacgc caagatcaag
     9061 gaacggctcg acgccggcga ggagatgccg gcatacctca aggaccaccc ggtctattac
     9121 gcgggcccgg ccaagacacc cgaggggatg ccgtcgggct cgttcggccc gacgaccgcc
     9181 ggccgcatgg actcctacgt cgaccagttc caggccgccg gtggctcgat ggtcatgctg
     9241 gccaagggca accggagcca gcaggtgacc gatgcctgcc aggcccacgg cggcttctat
     9301 ctcggctcga tcggcggccc ggcagcccgg ctggctcagg actgcatcaa gtcggtcgag
     9361 gtgctcgagt atcccgagct cggcatggag gccgtgtgga agatcgaggt cgaggacttc
     9421 ccggcgttca tcgtcgtcga cgacaagggc aacgacttct tcgcctcggt gaccaagccg
     9481 gtggccttca gcatcggcaa gcggccgggc ctggcttgag cggcgagaca ggagtcgaga
     9541 atgccagtgt cacagcgtca taggccgcag ccagggaccc gtccgcgcac gcccgaggag
     9601 gcttggcacc atttgatggc gggcaatgcc cggttcatgg ccgacgaacg cgcgcacccc
     9661 catcaggaca tggatgtgcg tcaccggctc gcccagaagc aggcaccctt cgcggtcttc
     9721 ttcggttgtg gcgactcccg cgtcgcagcg gagatcatct tcgaccaggg cctcggggat
     9781 ctgttcgtgg tgcgcaccgc cggtcacgtc gtcgacttca gcgtcctggg ctccatcgag
     9841 ttcggggtcg gcaatctcgg ttcacccttg atcgtcgtcc tgggtcacga ctcgtgcggc
     9901 gccgtcgcgg ccgccatcgc ctcggcccga tcgggccaga tgcccaaggg ctatgtccgt
     9961 gacatcgtcg agcgcgtgac accgagcgtc ttcgccgtcg cccagcgcgg tgagctgacg
    10021 ccggaatccg ttgagcgcga acatgttcgg gtcacgtgcg agttgctgta cgagcgatcg
    10081 atgatcatcc aggacgctgt cgacgccggg agtttggcga tcgtgggctg tgagtaccgg
    10141 ctggtcgatg gcgcggcaca tctcgtgcac gtcatcggtg acgtcggcga tgaggtggag
    10201 gtcagcgacg gcgacagccg gatctgagct gccggtcagc ggccgaggct ctccttgacg
    10261 acgccggcga ggcgttcggc gacggcctgg gccgcggcgg gggtggcagc ctcgaccatg
    10321 acgcgcacca gcggttcggt cccggacttg cgcagcagca cgcgtccgga ccccgccagc
    10381 gccgactcct catccgctac cgcggactgc agcctcggat cggtgtcgac ccggctggcg
    10441 tccacgccct tgacgttgat gagcacctgc ggcaggcggg tcatcacgct ggccagttcg
    10501 gcgagcggct tgcctgtttc ggcgacgcgc gccgcgagca tgaggccggt caggacgccg
    10561 tcgccggtcg tcccgtggtc gagcaggatg acgtggcccg actgctcccc gccgagggag
    10621 aagtcgttct cgcgcatggc ttccaggaca taacggtcgc ccacgccggt cttgacgacg
    10681 gtgatgccgg cgcggtccat cgcctgcagc agaccgaggt tcgacatgac cgtggccacg
    10741 agggtgtccg ccttgagcgt gccgcgctgc tgcatggcga gcgccaggat cgccatgatc
    10801 tggtcaccgt cgaccaccgt gcccgtgtgg tccactgcga gacagcgatc ggcgtcgcca
    10861 tcgtgcgcga ttccgaggtc ggcctcgcgg tcgacgacga cggcctggag atgctcaagg
    10921 tgcgtggagc cgtagccgtc gttgatgttc agcccgtcgg gagcggtgcc gatctcgatg
    10981 acgtcggcgc cggccagccg gaagacctcg ggggaaacag cgctggcggc cccgtgcgca
    11041 ccgtcgatga cgatggtgag cccgtcgagg cggttgggca cggcggcgag gaggtgcgcg
    11101 acataccgtg cctgtccggc ccggttggtg tggacgcgac cgacctcggc gccgatcggg
    11161 cgggtccact cttggtggag acgctcagcg atgtcgtcct cgacggcgtc cggcagcttg
    11221 tgcccgccgg cggcgaagaa cttgatcccg ttgtcgggca tggcattgtg cgacgccgag
    11281 agcatcacgc cgaaatgcgc gtccatgtcg gccgtcagga acgcgacggc gggagtaggc
    11341 aggaccccga cgtcgttgac gtcgacgccc gctgaagcca gcccggcgac gaccgcggcg
    11401 gagaggaact cccccgaggc gcgcgggtcg cggccgacca cggccttcgg cttgcggccg
    11461 gcggcgcgcg cgggctcgcc gagcacgtgc gccgccgcga cggacagtcc caacgccagc
    11521 tcggccgtga tgtccttgtt cgccaggccg cgaacgccgt cggtgccgaa gagacgtgcc
    11581 atgagtggcc ttccgctggg ggatcggttt cggccgcccg gtggggaggc cccaagaatg
    11641 gtagtccgcc gcgagctact gcccgtatgc cgccgcgcca ccaaccgctc atccgggcgt
    11701 cccccatttc ggtgtcacga ggtgcactcg tgctgatctc ggcggctgcg gacccgtgcc
    11761 gccggcgcga ggcggacggc tgggtgggag cggctagacg tcctgcggta gggcggcgag
    11821 cgcctccctg gcccacgtca ggtgagcgcg cgcgtgatgc tcgccgtagg acaaggtgag
    11881 cgagcgaaag gtgcgcgcct cgctctcggg ctccgccacc acctcggccc ggatcgcggc
    11941 gaatcgcccc aatgccgttg tggctcggtc gatttcggcc cgaagcaact ccgcggcgcg
    12001 ccctggcgga agctcgcttc cgaagaacag gcgcagcaac agcgcattcc ggggcggagg
    12061 cgcggtatgc ggttcggcca gccgctcgcg cagcacggcc cggccggcgc tcgtgatctc
    12121 gaaacggctc ccggaggtcc gccccggggt cgcccggcgc acggagccgc tctcctcgag
    12181 cgtggcgagc gtcggataga tctggccgaa gctctcgtgc cagaagtctc cgaggacgtc
    12241 ggtgatggca gcgcgcagct cgtatcccgt catggggccg atggacaggg caccgaggat
    12301 ggcaatttcc gtctgcgggc gacgtgacat cgcgccatgc nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn gcccagcgga ttcgagcgct atggctgggc gcctgtatgc
    12481 ctggtgctgg cgctggcggc gtggcgtagc cgacagccgt gaagcccagc acacctggtg
    12541 tccagaaacc gaagacgcgg ccctcgccga agcgaggacc gcgtctttcg gtgctgaaga
    12601 tcagcgcttg ctgtactgcg gcgccttgcg ggccttcttc agaccggcct tcttgcgctc
    12661 ggggacgcgg gcgtcccggg tgaggaagcc ggccttcttc agcgccgggc ggttgagctc
    12721 cgcgtcgacg ccgttgaggg agcgggcaac gccgaggcgg acggcgccgg cctggccgga
    12781 ggggccgccg ccgtgcacgc gcacgaggac gtcataggcg ccgtcgagct cgaggacggc
    12841 cagcggctcg ttgacgatct gctggtgcac cttgttgggg aagtagttct ccagggtgcg
    12901 accgttgatg gtccactcgc cggtgccagg gacgatgcgc acccgggcga tggcctgctt
    12961 gcggcggccg gtgccggcgc cgggggcgga ggcggtgggc ttgcgcgccg gctcgtcgga
    13021 gacgggaccc tcggactccg aggtgtactc ggtgagctcg acgtcctcgt cgtggtcgta
    13081 ggtggtgtca gtcacggctg tgaaaatcct tgtcttgtcg ggagatccgc gccgttactg
    13141 cgcgacctgg gagatcgtgt aggggaccgg cttctgggcc gtatgcgggt gctcggcacc
    13201 ggcgtagacc ttcagcttgc tcagctgctg gcgaccgagg gtggtcttgg gcagcatgcc
    13261 gcggacggcc ttctcgacgg ccttggccgg gtcgcggtcg agcagctggg cgtaggtctc
    13321 ggacttcagg ccgcccgggt agccggagtg gcggtaggcc cgcttctgct gcagcttggc
    13381 gccggtgagg gcgaccttgt cggcgttgat gatgatgaca aagtcgccgg tgtcgacgtg
    13441 cggggcgaag gtcgccttgt gcttgccgcg caggagcgca gccgtctggc tggcgagccg
    13501 gccgagaacg acgtcggtgg cgtcgatgac gtgccactga cgggtgatct ccgccgcctt
    13561 gggggtgtag gtacgcaaag ttttgccttt acttacgctt cggatagggc gtggtatgcc
    13621 gcgtgctccc gccgggcggg tgaggtcgcc caccaggggc gcacggcaca acagccatcc
    13681 attttacggg cggcacgcct acgccccaaa tccgcctttc agccctccgc cgcgcactgg
    13741 acctgcgcaa acgcgcctcc atacattctt tgcatactac ttgtgcaaag gaattgtgca
    13801 caagtagtat gcaatctatg accgactcgg cctcctatat ccggctcgac tccacctccg
    13861 tcaaggtgct cgcgcacccc ctgcgctcgc gcctcgtcgg cgcgctgcgg ctgcacggtc
    13921 cctcgaccgc gaccgccctg gccaaggtgc tgtcgacgaa ctcgggggcc acgtcatacc
    13981 acctgcgaaa gctcgccgaa gttggcctgg tcgtcgacac gggcgagggc gatggcaaat
    14041 cccgcctgtg ggcagccgcc tccgagacga cccagtactt gagcagtgac ttttccgagg
    14101 acgaggacgc cgcgacggct cttggctggt tggagcggga ctggctgaag catttcaccg
    14161 agaagttcgg gcgatggctc gatgtccggc aggggtggcc ggcggtatgg cgcgaccggg
    14221 cgagcatgaa cgacgacacc gtgttggtga cggccgacca gttgcgcgca ctgcacgcgg
    14281 agatcgacga ggtggtggac cgctatcgac gcgtgggaca gggcaatccg gacgccaagc
    14341 gaatcgcgac ctatctggtc ttctatccgg tcgacatgga caaggccccc cgcgcgtgac
    14401 gaccatggag acgaccacac cgcccaccat gagtccggcg cacgcgcggc gcgtcttcta
    14461 catc
//
LOCUS       DAOPJG010000074        14374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4412_length_14374_cov_7.676584, whole genome shotgun sequence.
ACCESSION   DAOPJG010000074 DAOPJG010000000
VERSION     DAOPJG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4412_length_14374_cov_7.676584"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKU75_08460"
     CDS             <1..358
                     /locus_tag="PKU75_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80947.1"
                     /translation="AAASAAAAGGAAAGAAAGSAGAGMSVPPPPPAPAPAAPAPAVPE
                     EAAAPEAGAQESSPAAPTPEAAPERVWEDATMDATPDADVLAAPDGELVDVEEEVTDL
                     GGGGGDTPPPPPPSWA"
     gene            543..3419
                     /gene="leuS"
                     /locus_tag="PKU75_08465"
     CDS             543..3419
                     /gene="leuS"
                     /locus_tag="PKU75_08465"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPF80948.1"
                     /translation="MSETPHRYTADLAQQIEVAWQDRWEEEGTFHAPNPAGSLADPDA
                     VARFPGGHLLVLDMFPYPSGAGLHIGHPLGYIATDVFSRYQRMLGKNVCHCIGYDAFG
                     LPAEQYAVQTGQHPRKTTDENIAIMKRQLRRLGLSLDDRRAIETIDPGYYTWTQWIFV
                     QIFNAWYDADAARPDGGTGRARPIADLVAQFASGERATPGGRAWDELSEVERAEILGA
                     HRLVNSVEAQVNWCPGLGTVLANEEVTADGRSDRGNFPVFKRNLRQWVMRITEYADRL
                     AEDLDRVDWPEKVKVMQRNWIGRSEGASVTFPVAGSDAGIEVFTTRPDTLFGATFMVV
                     SPEHPIVDSLVPQGDWPTETKPAWTGGAASPATAVAAYRRAAASKSDIERQENKDKTG
                     VFTGAFALNPVNGQRIPVFVADYVLMGYGTGAIMAVPAQDQRDWDFAVAFDLPIVRTV
                     QPAPDHDMDTAFTGDGPAINSANDEVSLDGLGVADAKARITAYLESKGAGRGTVTYKL
                     RDWLFARQRYWGEPFPILFDENGIAHAVPEAMLPVELPDIPDYSPRTYDPEDAESSPE
                     APLARNADWVNVRLDLGDGAGERDYRRDTNVMPQWAGSCWYYLRYLDPLNSEAFVDAT
                     NERYWIGPRENPVVAGTEDPGGVDLYVGGVEHAVLHLLYARFWHKVLYDLGHVSSSEP
                     FRTYFSQGYIQAPAYKDARGQYVEAKEVVEAHDGSFSWGGEPVTQEFGKIGKSLKNMV
                     SPDDMYGAYGADTLRLYEMGLGPLAQSKPWDTRAVVGSQRFLQRLWRNVIDEEAGTLR
                     VVDSAPDPETLRVLHRTIAAVRADYEGLGFNTAIARLTELNNALTKLDEVPRGIVEPL
                     VLMTAPLAPHIAEELWKRLGHSETLTYEPFPVADEQWLVEDTVTCVVQIRGKVRDRIE
                     VAPSIGEDELRERVLALPKVVEATPDGTRTVIVRAPKLVNVVPN"
     gene            3622..4614
                     /locus_tag="PKU75_08470"
     CDS             3622..4614
                     /locus_tag="PKU75_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HPF80949.1"
                     /translation="MTFRQRDDPAERLRVLLDAGRSRAPGDPEPPDADWWDDADDADD
                     IDEPGDGEVGALGSDQRYDARSGARAPAGARHARPPAITAPLGLRERFTAVSHPVVVT
                     VLLAAVAAGLLFGMRLVRARADAVPIPALSTMTDPRATGGGEPAAAGGASGDSRVSGS
                     SAPGPPATDISSGGSASAGAMVRVHVVGQVKRPGVVEVSAGARILDAIEAAGGATSKA
                     DLDAINLARAVVDGEQIQVLRPGESPTPASGPAAPATGSGSGGTGGGPGVKVPINTAD
                     LAQLDTLPGVGPVLAQRILDWRSQHGAFASIDQLGEVSGIGEKLLANLTPLVTL"
     gene            4618..7038
                     /locus_tag="PKU75_08475"
     CDS             4618..7038
                     /locus_tag="PKU75_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPF80950.1"
                     /translation="MWGQTRDGSVRLDPRGHARQTRPGDGHPDRRPGVDLRLLVPALF
                     GWGATALTLGVGGGIRVIIGAVALVVAAWPAWLGRARHRRGGGALRMAALVAAVTGVL
                     VATAGLADLRRRAGVVDELANRGGVGRISGPVVAEPVPIDNGRFVVRLRAETVLSAGQ
                     VTRSSATMLVIGDDRWSHLQWRDHVDVLVRMRPADPGDSVLAIAKPLYGPTSRPASSR
                     LVAWSSHIRGALRGAVSALPPDAQGLVPAMVFGDTSRSPPELTSDMRAAGLAHLSAVS
                     GANVSILVVCAVAGLGVFGVRRRWRPAALLLVIAAFVVVTRPEPSVIRAAAMGGVGVL
                     ACYRSTRAAGIPALGAAIVGLLMADPWLARSFGFALSALATLGLLVFAGPWSRAVVQR
                     FPRCPAPIAVAVVVPVAAQVTTAPVLVLLQGNVSVVGIPANILAAPFVAPVTIAGLGL
                     ATLAALGLPTGWLAWAPGAPALAITWIAHRAAGVSWGSIDWPDGAAGAMLLTGVTLLL
                     LVTAPRLRYAARVRPRTSLAGLLLSLAAVLPTGWWGRPPGPWQLVACDVGQGDALVVA
                     TTPGRGILVDAGPEPLAVDRCLRRLSVKALDLIVLTHFHADHVDGLAGALRDRSVAGI
                     VTSPVADPPDRAATVARLAGQGGIPLRPARLGEQLRFGATTLSVLGPARRIDAGSVPN
                     NASVVLTVRIGDFDALLTGDVEAEAGRSLRLTQQANLFGPQSNPRFDVVKAPHHGSAN
                     YDDILFAETRAPVTLVSVGADNDYGHPAPRFLDAAAALGTRVLRTDQAGDIALWVAGG
                     QLYVRTDH"
     gene            complement(7131..8108)
                     /locus_tag="PKU75_08480"
     CDS             complement(7131..8108)
                     /locus_tag="PKU75_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPF80951.1"
                     /translation="MIDAAGLRVMRAIAEQGSFTGAATALGYSQPAISQMVRRLEQRT
                     GTPLVDRVGRTVRLTEAGAALARHAGTVLSALDAAEEEVSAIAGLRAGRVRLAAFPSA
                     SATLVPKALALVRDRFPDISMTFTEAEPPESLAALRAGECDLAVAFTYAGDDLAADSE
                     DLDVLVTEPLLRDEVLIALPPSHRLARGTGPVDLADLATEHWIAGCPRCRGHLLTMCD
                     QAGFSPDVSFETEDYVAVQGLVAAGLGVALIPDLIVRTTYNPDVVIRQVTPASRRDIL
                     AVTTPDLRRVPAVAAALEALAEVAASPDLLPTFPGDAASVPEPELDYHL"
     gene            8224..9105
                     /locus_tag="PKU75_08485"
     CDS             8224..9105
                     /locus_tag="PKU75_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HPF80952.1"
                     /translation="MHDWTHAAAELADLLEDRPALVLSGAGLSTDSGIPDYRGPDGTR
                     RVTPMSHGEFVTSPAGRQRYWARSYAGWERFSAARPNDGHRAITTLQQAGRVGAIISQ
                     NVDGLHQAAGARDVIDLHGRLAEVVCLTCDARLERSAVQEWMATANPDFARTVALAPG
                     GGSRVSAQIRPDGDVVLDDAAVAGFRAPRCPVCRGDALKPDVVFFGGSVPKERVERCF
                     ALTDQARALLVVGSSLMVMSGLRFVKRAAAHGIPIAVLTKGPTRGDDLATLKIDAPIT
                     PVLSRLAGAVRPGAAPR"
     gene            complement(9080..9817)
                     /gene="lexA"
                     /locus_tag="PKU75_08490"
     CDS             complement(9080..9817)
                     /gene="lexA"
                     /locus_tag="PKU75_08490"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPF80953.1"
                     /translation="MSTKDGKGQVHPMPERSDGDGLTVRQRRVLEVIRNSIDRRGYPP
                     SMREIGEAVGLVSPSSVAYQLHALENKGYLRRDPHRPRAIEVVSPDAPRKRGYRQTAK
                     PAPAPEEFVDETGSGDARPEASYVPVLGRIAAGVPITADEVVEDVFPLPRQIVGEGSL
                     FLLKVVGDSMIDAAICDGDWVVVRQQPTADNGDIVAALLDNEATVKTFKRKNGKVWLM
                     PHNDAFSPIDGDHATILGKVTAVLRRV"
     gene            10041..10496
                     /locus_tag="PKU75_08495"
     CDS             10041..10496
                     /locus_tag="PKU75_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPF80954.1"
                     /translation="MGAALAQVNGGALGEGPWGARPRRMASPGGPRRAHLTVVPDLPA
                     VPPSRGGRATGVVKLTRLGRLAITLMAAAVLLGTTVLAASAAGVGPFGGPVSGERIVV
                     VESGQTLSGLAESYLPGRVIGDGVAAIQIANGLSSEHVQAGQRLVIPAR"
     gene            10714..11205
                     /gene="nrdR"
                     /locus_tag="PKU75_08500"
     CDS             10714..11205
                     /gene="nrdR"
                     /locus_tag="PKU75_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPF80955.1"
                     /translation="MHCPFCRHTDSRVVDSRTSDDGAQIRRRRQCPDCGRRFTTIETA
                     GLTVLKRSGASEPFSRAKVLAGVRKACQGRPVTEDQLAVLAQRVEEGVRAAGNAEVDA
                     HEVGMAILGPLRELDEVAYLRFASVYQAFESLEDFESAITLLRAEREAAAPDGRPDRI
                     PAP"
     gene            11298..14216
                     /locus_tag="PKU75_08505"
     CDS             11298..14216
                     /locus_tag="PKU75_08505"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPF80956.1"
                     /translation="MTETARSNAARSGKAKGLKVERIFTTAGVHPYDEVTWESRDVVQ
                     QNWKTGETIFEQRGVEFPDFWSVNASTIVTTKYFRGAVGTEARERSLRQLIDRVVLTY
                     TKAGKQHGYFATEQDAEVFEHELTYALLHQVFSFNSPVWFNVGTPSPQQVSACFILSV
                     DDSMDSILNWYKEEGKIFQGGSGAGLNLSRIRSSKELLSSGGTASGPVSFMRGADASA
                     GTIKSGGATRRAAKMVVLDVDHPDIVEFVETKAREEDKIRALRDAGFDMDLGGKDIVS
                     VQYQNANNSVRVTDEFMRAVEEGTEFNLTSRLDGSVVESVDARELMRKISEAAWECAD
                     PGMQYDDTINAWHTNPETGRITASNPCSEYMSLDNSSCNLASLNLLKFLREDDTFDAS
                     TFQKVVELVITAMDISICFADFPTEPIGKTTVDYRQLGIGYANLGALLMATGHGYDSE
                     GGRALAASITSLLTGAAYKRSAEMAGVVGPYAGYARNADAHKRVMRKHQAANDAVRTR
                     HTMDADIHKLATRAWDDVLTLGERNGFRNAQASVLAPTGTIGFMMDCDTTGIEPDFSL
                     VKFKKLVGGGSMQIVNLTIPRALRKLGYAEETVEAIVEFIADKGHVVGAPGLKTEHYE
                     VFDTAMGERAISAMGHVRMMASVQPFLSGAISKTVNLPESASVEEIEDVYVQGWKMGL
                     KALAVYRDNCKVGQPLSDAGSTAKDKEAAAAAPVEKVVEYRPVRERLPKRRNSRTTSF
                     AIGGAEGYMTASTYEDGRLGEVFLKFGKQGSTLAGVMDAFSIAISVALQYGVPLETWV
                     EKFTNMRFEPAGMTDDPDVRMGQSLMDYVFRRLALDHLDFETRSYYGIYTAEERARQL
                     ETGSYAPVDEESEEDIEDEMEIMQEGVGAQAPKKTAEPKQSPHHDKDLDATKVQSGSG
                     AASARKVDADVHSSADLLAKFGNSKAADAPMCMTCGTKMRPAGSCYVCEGCGSTSGCS
                     "
BASE COUNT         2168 a         4948 c         5002 g         2256 t
ORIGIN      
        1 ggccgccgcg agcgccgctg ccgctggtgg cgctgctgct ggtgccgctg ccggctccgc
       61 cggagcgggt atgtccgtgc ccccgccccc tccggccccg gcgccggccg ccccggcgcc
      121 ggctgtcccc gaagaagccg ccgcccccga ggccggtgct caggagtcct cccccgccgc
      181 gccgactccc gaggcagcgc cggagcgggt ctgggaggac gccaccatgg atgccacccc
      241 ggacgccgac gtgctcgctg cccccgacgg cgagctcgtc gacgtcgagg aagaggtcac
      301 cgacctcgga ggcggtggcg gcgacacgcc cccgccacct ccgccctcct gggcgtgagg
      361 accccccgga atcggggcgg ccgctctgcc ggcctgaggt aacctggggg ctatgacagg
      421 ccggcagctg cttagtcagc cgcggtgacg tgaccggata ggacgtcaac gcggccatac
      481 cccttcgccc accgaggggg tatttctatg cccgagcagc accggatcga cgagagagac
      541 cgatgagcga gactccgcac cgctataccg ccgacctggc ccagcagatc gaagtggcct
      601 ggcaggaccg ttgggaggag gaggggacct tccacgcgcc caaccccgcc ggctcgctcg
      661 cggacccgga tgcggtcgcg cgcttccccg gcgggcacct gctcgtgctg gacatgttcc
      721 cctaccccag cggtgcgggg ctgcacatcg gtcacccgct gggctatatc gccacggacg
      781 tcttcagtcg ctaccagcgg atgctgggca agaacgtgtg tcactgcatc ggctacgacg
      841 ccttcggcct gccggccgag cagtacgccg tccagaccgg tcagcacccc cgcaagacga
      901 ccgacgagaa catcgcgatc atgaagcggc agctgcgccg cctgggcttg agcctggacg
      961 accgccgcgc catcgagacg atcgacccgg gctactacac gtggacccag tggatcttcg
     1021 tccagatctt caacgcctgg tatgacgcgg acgccgcccg tcccgatggt ggcaccggcc
     1081 gggcgcggcc catcgccgac ttggtcgcgc agttcgcctc cggcgagcgg gcgactcccg
     1141 gcggccgcgc gtgggacgag ctgagcgagg tcgagcgcgc cgagatcctg ggcgcccatc
     1201 gtctggtgaa ttccgttgag gcacaggtga actggtgtcc cggcctgggc acggtgctgg
     1261 ccaacgagga ggtcacggcc gacgggcgct ccgaccgggg caacttcccg gtgttcaagc
     1321 gcaacctgcg tcagtgggtc atgcggatca ccgagtatgc cgaccggctg gccgaggatc
     1381 tcgaccgcgt ggactggccg gagaaggtca aggtcatgca gcgcaactgg atcggccggt
     1441 ccgagggcgc cagcgtcacc ttccccgtcg ccggttcgga cgccggcatc gaggtcttca
     1501 ccacccgccc ggacacgctc ttcggggcaa cgttcatggt cgtctcgccc gagcacccca
     1561 tcgtcgactc ccttgtcccg caaggcgact ggccgaccga gaccaagccg gcgtggacgg
     1621 gcggtgcggc ttcgccggcg acggcggtcg cggcataccg tcgggcggcg gcgagcaaga
     1681 gcgacatcga gcggcaggag aacaaggaca agaccggcgt cttcaccggt gccttcgcgc
     1741 tgaatcccgt caacgggcaa cggatcccgg tgttcgtcgc cgactacgtc ctgatgggct
     1801 acggcaccgg cgcgatcatg gccgtgccgg cgcaggacca gcgcgactgg gacttcgcgg
     1861 tcgccttcga cctgccgatc gtccgcaccg tgcagccggc gcccgatcac gacatggaca
     1921 ccgctttcac cggcgacggg cccgcgatca actccgccaa cgacgaggtc tcgctcgacg
     1981 ggctgggcgt cgccgacgcg aaggcgcgga tcacggcata cctggagagc aagggggccg
     2041 gccgcggcac ggtcacctac aagttgcgcg actggctctt tgcgcggcaa cgctattggg
     2101 gcgaaccctt cccgatcctg ttcgacgaga acggaatcgc gcacgccgtg cccgaggcga
     2161 tgctgcccgt cgaactgccg gacatccccg actactcgcc gcgcacctac gacccggagg
     2221 acgccgagtc gtcgccggag gcgccgctgg cgcgcaacgc cgactgggtc aacgtgcgtc
     2281 tcgacctggg cgacggtgcg ggggagcggg actaccgccg cgacaccaac gtcatgccgc
     2341 agtgggccgg atcctgttgg tattacttgc gttatctgga tccgctgaac tccgaggcgt
     2401 tcgtcgacgc gaccaacgag cgttattgga tcgggccgcg ggagaacccc gtggtcgccg
     2461 gtaccgagga tcccggtggc gtggatctgt atgtcggcgg cgtcgagcac gccgtgctgc
     2521 acctgctgta tgcgcgcttc tggcacaagg tcctctacga cctggggcac gtcagttcca
     2581 gcgagccgtt ccgcacctac ttctcccagg gctacatcca ggcgccggcc tacaaggatg
     2641 cgcgggggca gtatgtcgag gccaaggagg tcgtcgaggc gcacgacggc agcttctcgt
     2701 ggggcggtga gccggtcacg caggagttcg gcaagatcgg caagtcgctg aagaacatgg
     2761 tctcgccgga cgacatgtac ggggcgtatg gcgccgacac gctgcgcctg tacgagatgg
     2821 gtctgggtcc gctggcgcag tccaagccgt gggacacccg ggccgtcgtc ggcagccagc
     2881 gcttcctgca acggttgtgg cgcaacgtga tcgacgaaga ggccggcacg ctgcgggtcg
     2941 tcgactcggc tcctgatccc gagacattgc gggtgctgca ccgcaccatc gcggcggtcc
     3001 gcgcggacta cgagggcctg ggcttcaaca ccgccatcgc ccggttgacc gagctcaaca
     3061 atgcgctgac gaagctggac gaggtgccgc gtggcatcgt ggagccgctg gttctgatga
     3121 ccgccccgct ggcgccgcac atcgccgagg aactgtggaa gcggctgggg cactccgaga
     3181 cgctgacata tgagccgttc ccggtcgccg acgagcaatg gctggtcgag gacaccgtca
     3241 cctgcgtcgt gcagatcagg ggcaaggtcc gcgatcgcat cgaggtggca ccgagcatcg
     3301 gtgaggacga gctgcgcgag cgggtcctgg cgctgccgaa ggtggtcgag gcgacccccg
     3361 acgggacgcg cacggtgatc gtgcgcgcgc cgaagctggt caacgtcgtc ccgaactgat
     3421 caccggtgct gacggtgcgg agatgcgcat tgtccatgtg gcatggttgc cggtcgtgcc
     3481 ggctcgatcg ctgtcgatga ggcacctgat tcgtcgcgac cgggaggaac ttgtccacag
     3541 cgcggtggct gacttcgcat aatccacagg ccttttcggg gggactggcg gggcgttgat
     3601 cgggttcgta cgttggcgga catgactttt cgccagcggg acgacccggc cgagcggttg
     3661 cgggtgttgc tcgacgcggg tcggtcacgc gcgccgggcg atcccgagcc ccccgatgcc
     3721 gactggtggg acgacgccga cgacgccgac gacatcgacg aaccggggga cggcgaggtc
     3781 ggtgcgctcg gatcggacca gcggtatgac gcccggtccg gagctcgtgc tccggccggc
     3841 gcgcggcacg cccgaccacc tgcgatcacc gccccgctcg gcttgcgaga gcgcttcacc
     3901 gccgtcagcc atccggtggt ggtcaccgtt ctgctggcgg cggtggctgc cgggttgctc
     3961 ttcgggatgc ggctcgtgcg ggcgcgggcg gacgcggtgc ccatcccggc actgtccacg
     4021 atgacggacc cgcgggccac gggcggcggc gagccggccg cggccggcgg cgcgtccggg
     4081 gactcccgtg tgtcaggctc aagtgcgccc ggtccgcccg cgacggatat ctcttcgggt
     4141 ggctcggcga gcgccggggc catggtgcgc gtccatgtcg tgggccaggt caagcgtccc
     4201 ggggtcgtgg aggtgtccgc gggggcgcgg atcctggatg cgatcgaggc ggccgggggc
     4261 gcgacgagca aggcggacct ggacgcgatc aacctggccc gcgcggtggt cgacggcgaa
     4321 cagatccagg tccttcggcc tggagagtcc ccgactccag cgtccgggcc ggcggcaccg
     4381 gccaccggat ccgggtcggg cggcacaggc ggagggcccg gagtaaaggt gcccatcaac
     4441 acagccgacc tggctcaact cgacaccctg cccggcgtgg gtccggttct cgcgcaacga
     4501 atcctggact ggcgcagcca gcacggcgca ttcgcgagca tcgatcagct cggcgaggtc
     4561 agcggcatcg gcgagaagct gctggccaat ctcaccccgc tcgtgacgct ctagacgatg
     4621 tggggacaga cgcgcgacgg cagcgttcgg ctcgacccgc gtggccacgc tcggcagacg
     4681 cgtcccggcg acggccaccc ggaccgccgc ccgggtgtcg atctgcggct gctggtgccg
     4741 gcgctgttcg ggtggggagc aaccgccctg accctgggcg tcggcggggg gatccgcgtg
     4801 atcatcgggg ctgtcgccct ggtcgtggcg gcctggcccg cgtggctcgg ccgggcccgg
     4861 catcgccgag gtggtggggc gttgcggatg gcggcactgg tggccgccgt gaccggagtt
     4921 cttgtcgcca cggcgggcct ggccgacctg cggcgccgcg ccggggtcgt cgacgaactc
     4981 gcaaatcgag gcggcgtcgg ccgcatctcc gggccggtcg tggccgagcc ggtgccgatc
     5041 gacaacggcc ggttcgtggt gcgcctgcgt gccgagacgg tgctcagcgc cgggcaggtc
     5101 acgcgttcgt ccgcgacgat gctcgtcatc ggggatgaca ggtggtcgca tctgcagtgg
     5161 cgcgatcacg tcgacgtgct ggtgcggatg cggcctgccg accccggcga ttccgttctc
     5221 gccatagcta aaccgttgta cggacccacg agccggcccg cctcgtcccg gctcgtggcg
     5281 tggtcctccc atatccgcgg ggcactgcgc ggtgcggtgt cggccttgcc gccggacgcc
     5341 cagggcctgg tcccggccat ggtgttcggc gacacctccc gctccccgcc cgagctgacc
     5401 tccgacatgc gcgccgcggg tctggcgcac ctgtcggcgg tcagcggcgc caacgtcagc
     5461 atcctcgtcg tgtgtgccgt ggccgggctg ggggtgtttg gggttcgccg gcgctggcgg
     5521 cctgctgcgc tgctccttgt catcgcggcc ttcgtcgtcg tcacccgccc ggagccgagc
     5581 gtcatccggg ccgccgcgat gggcggcgtc ggagtcctgg cgtgttaccg atcgacgcgg
     5641 gcagccggca taccggctct cggcgcggcc atcgtcgggc tgctgatggc ggacccgtgg
     5701 ctggcgcgca gcttcgggtt cgcgctatcg gcgctggcga ccctggggct gctggtcttc
     5761 gccggcccgt ggtcgcgggc cgtggtgcag cgcttcccgc gttgcccggc gccgatcgcc
     5821 gtggcggtcg tcgtcccggt tgccgcgcag gtgacgactg ccccggttct cgttctcctg
     5881 caagggaatg tcagcgtcgt ggggattccc gcgaatatcc tcgccgctcc cttcgtggcg
     5941 ccggtgacga ttgccggtct cggtttggca acccttgctg cgctcggcct gccgaccggc
     6001 tggctggcct gggcgccggg cgcaccggca ctggccatca cctggatcgc ccaccgtgcc
     6061 gccggcgtgt cgtggggcag catcgactgg cccgacggtg ccgccggcgc gatgctgctg
     6121 acaggagtga cgctgctgct cctcgtcacc gcccctcggc tgcggtatgc cgcacgcgtg
     6181 cgcccccgca cgagtcttgc cgggctgttg ctctcgctgg ctgccgtgct gccgaccggg
     6241 tggtggggcc ggccacctgg gccatggcaa ctcgtggctt gcgatgtcgg ccagggtgat
     6301 gctctcgtcg tcgcgacgac acccggccgc ggcatcctcg tcgatgcggg tccggagccg
     6361 ctcgctgtcg accgctgcct gcggcggctc agcgtcaagg cgctggacct gatcgtcctg
     6421 acccacttcc acgccgatca cgtcgacggc ctagccggag ccctgcgcga ccgatccgtc
     6481 gcgggcatcg tcacctcacc cgtcgcggac ccacccgacc gagctgccac cgtcgcgcgg
     6541 ctggccgggc agggcggcat acccctacgt cccgctcggc tcggcgagca gctccggttc
     6601 ggcgccacga cgctgagcgt cctcgggccc gcgcgccgca tcgacgccgg ctcggtcccc
     6661 aacaacgcga gcgtcgtgct gaccgtgcgc atcggggact tcgacgccct cctcaccggg
     6721 gatgtcgagg cggaggcggg ccgttctcta cgcctcaccc agcaggcgaa cctctttggc
     6781 ccgcagtcga atccacggtt cgacgtcgtc aaggctcccc accacgggtc ggcgaactac
     6841 gacgacatcc tgttcgctga gacccgggcg ccggtgacgc tggtcagcgt cggcgccgac
     6901 aacgactacg gccatcccgc cccacgcttc ctcgatgcgg ccgcggcgct cggcacccgg
     6961 gtcctgcgca ccgaccaggc cggcgatatc gccctctggg tggccggcgg ccaactctac
     7021 gtccgcaccg atcactagcc ccccgtcgag atgtcgattc ccaccccgtt gagatatcac
     7081 tccgcctcac ccgtcacctc cgtcatgccc ggggactgcc cggcccggcg tcagaggtga
     7141 tagtcgagct cgggttcggg aactgacgca gcgtcaccgg ggaaggtcgg caggaggtcg
     7201 ggtgaggcgg cgacctcggc cagcgcttcg agcgcggccg cgacggccgg cacccggcgc
     7261 aggtcgggag tggtcacggc caggatgtcc cggcgggaag ccggcgtgac ctggcggatg
     7321 acgacgtcgg ggttgtaggt ggtgcgcacg atgaggtcgg ggatgagtgc gacgccgagc
     7381 cccgccgcca ccagcccttg gaccgcgaca tagtcctcgg tctcgaaact gacgtcggga
     7441 ctgaacccgg cctgatcgca catggtcagc aggtgacctc ggcatcgggg acagccggcg
     7501 atccaatgtt ccgtggctag gtctgcgaga tccaccggcc cggtgccccg ggccagtcgg
     7561 tggctggggg gcaaggcgat cagcacctca tcccgtagga ggggttcggt gaccaggacg
     7621 tccagatcct cgctgtcggc ggccaggtcg tcaccggcgt aggtgaaggc cacggccagg
     7681 tcacactcgc cggcgcgcag ggcggcgagc gattcggggg gttcggcctc ggtgaaggtc
     7741 atcgagatgt ccgggaagcg atcccgcacc agcgcaaggg ctttgggcac gagcgtcgcc
     7801 gatgcggatg ggaaggccgc caaccggaca cgaccggccc gcagcccggc gattgcggag
     7861 acttcctcct cggcggcgtc gagtgcgctg agaacggtcc cggcgtgccg ggccagggcg
     7921 gcacccgctt cggtgagccg gacggtgcgg ccgacccggt cgacgagggg agtgccggtg
     7981 cgttgctcga gccggcggac catctgtgag atcgcgggct gggaatagcc gagggcggtc
     8041 gccgcgccgg tgaagctgcc ctgctcggcg atggcgcgca tcacccgcag ccccgcggca
     8101 tcgatcatcc cgacatcata taacggcgtg ttatgcgtcg aggcatttcg tgtcggtgaa
     8161 ccgcagtatt cgcgtggtcc gcaggctgac ccgacggcgg gggcacaccg catactgctt
     8221 gtcgtgcacg actggaccca cgccgccgcc gaactggccg acctcctgga ggatcgcccg
     8281 gcgctcgtgt tgtccggggc ggggctgagc accgattcgg ggatccccga ctatcgcggg
     8341 ccggacggca ctcggcgggt gacgccgatg tctcatggcg agttcgtcac ctcccccgct
     8401 gggcggcaac gctattgggc ccggtcgtat gccgggtggg agcggttctc cgccgcccgc
     8461 cccaacgacg ggcaccgggc gatcacgacg cttcagcagg ccggtcgcgt cggcgcgatc
     8521 atcagccaga acgtcgacgg gttgcaccag gctgcgggtg cccgtgacgt catcgacctt
     8581 cacggccggc tggcggaggt cgtctgcctg acctgtgacg cgcggctgga gcgcagtgcc
     8641 gtgcaggagt ggatggccac ggcgaacccg gacttcgccc gcacggtcgc gctggcacct
     8701 ggcggcgggt cgcgggtgtc ggcgcagatc cggcccgacg gtgacgtggt gctcgacgat
     8761 gccgcggtcg ccggcttccg cgctccgcgg tgtccggtat gccgcggcga cgccctgaag
     8821 cccgacgtcg tcttcttcgg tgggagcgtg ccgaaggaac gcgtcgagag gtgcttcgcc
     8881 ctgaccgatc aggctcgcgc actgctcgtc gtcggctcct cgctgatggt gatgagcggg
     8941 ctacggttcg tgaaaagggc tgcggcacac ggcataccga tcgccgttct gaccaaaggg
     9001 cccacgcgcg gcgacgatct ggcgacgttg aagatcgatg cccccatcac gccggtgctc
     9061 agccggctcg cgggggcggt cagacccggc gcagcaccgc ggtgaccttg cccaggatcg
     9121 tcgcgtggtc accgtcgatg ggggagaacg cgtcgttgtg cggcatgagc cagaccttgc
     9181 cgttcttgcg cttgaaggtc ttgaccgtgg cctcgttgtc caggagcgcg gcgacgatgt
     9241 cgccgttgtc ggcggtgggc tgctggcgca cgacgaccca gtcgccgtcg cagatcgctg
     9301 cgtcgatcat cgagtcgccg acgaccttga gcaggaagag cgagccctcg cccacgatct
     9361 gccggggaag cgggaagacg tcttcgacga cctcgtcggc ggtgatgggc acgccggcgg
     9421 cgatgcgacc gagcaccgga acgtatgacg cctcggggcg cgcgtcgccg ctgccggtct
     9481 cgtcgacgaa ttcctcgggc gccggggccg gcttcgccgt ctggcggtag ccgcgcttgc
     9541 ggggagcatc cgggctgacc acctcgatgg cacggggccg gtgcgggtcg cgtcgcaggt
     9601 agcccttgtt ctcgagagcg tgcaattgat aggccacgga gctgggcgag accaggccga
     9661 cggcctcgcc gatctcacgc atgctgggcg gatagccgcg gcggtcgatc gagttgcgga
     9721 tgacctccag gacccgtcgc tggcggaccg tgagcccgtc gccgtccgat cgttcgggca
     9781 tcgggtggac ctggcccttg ccgtccttgg tgctcatgcc aacctccgct agcgcttgtc
     9841 cgcgtgaatt gcctggtcag acaccttgtc agtggtccct gtcaggcttt gttccatggc
     9901 atcgagccta gggcctcaag gtcacgaact caaacatctg ttcgaggtgt gtcttgcgtc
     9961 cgggatcgac tgcgtgctag acatcgtaca accgttctat cgaactgagt ttcgatagac
    10021 acgccggaag gagttacgac atgggtgcag cactcgcaca ggtgaatggt ggcgcactgg
    10081 gtgaggggcc ctggggggct cgtccgcgcc ggatggcgtc gccgggtggg ccgcggcgcg
    10141 cgcacctcac cgtggtcccg gacctgccgg ctgtcccgcc gtcacggggc gggcgtgcca
    10201 ccggcgtcgt caagctcacg cgtctcggcc gattggcgat cacgctgatg gccgccgccg
    10261 tgctcctcgg caccacggtc ctcgcggcgt ccgctgccgg cgtcggaccg ttcggcggac
    10321 cggtctccgg cgagcggatc gtcgtggtgg agtccggcca gacgctctcc gggctcgcgg
    10381 agagctacct gccgggtcga gtgatcggcg acggcgtggc ggcgatccag atcgccaacg
    10441 gcctttccag cgagcacgtc caggccggtc agcggctcgt gattcccgcc cgctgagcgc
    10501 gcccacgcgc agctgttcac ggcgccgaca cgccggacac gcgcacgaca cgcataggtc
    10561 ttgcgttagt gccgacccgg gcataaagtc ctacatgtag tggttacacc gatgtagttc
    10621 ttccacatgt agtgcacatg cgagggaagt tactcacacg ttgtccccac gttgtccccg
    10681 gattcgtccc caccacaccg atcggagttg gccgtgcact gtcctttctg ccgtcacacc
    10741 gactcccggg tcgtcgacag ccggaccagt gacgacggcg cccagatccg ccggcgccgc
    10801 cagtgcccgg attgcgggcg acggttcacc accatcgaga ccgcgggcct gacagtgctc
    10861 aagcgctccg gcgccagcga accgttcagc cgggccaagg tcctcgccgg ggtccgcaag
    10921 gcctgccagg gccgcccggt caccgaggac caactcgccg tcctcgccca acgggtcgag
    10981 gagggcgtgc gcgccgcggg caacgccgag gtcgacgccc acgaagtggg aatggcgatt
    11041 ctcgggccgc tgcgcgaact cgacgaagtc gcctacctgc gcttcgccag cgtctaccaa
    11101 gccttcgagt ccctcgagga cttcgagagc gccatcaccc tgcttcgcgc cgagcgcgag
    11161 gcggctgccc cggacgggcg accggaccgc ataccggccc cttaggactc ccgggtgccg
    11221 gccgaaccgc cacggccggc atccggtgac cacaacccca ccgcaccacc cgcacagtca
    11281 ccccaggaga gggatatatg accgagaccg ctcgctcgaa cgccgcacga tccggcaagg
    11341 ccaaaggtct gaaggtggag cgcatcttca ccaccgccgg cgtgcacccc tatgacgagg
    11401 tgacgtggga gagccgggat gtcgtccagc agaactggaa gacgggcgag acgatcttcg
    11461 agcagcgcgg cgtggagttc cccgacttct ggtcggtcaa cgcctcgacc atcgtcacga
    11521 ccaagtactt ccgtggcgcg gtcggcaccg aggcgcgcga gcgcagcctg cgccagctca
    11581 tcgaccgggt ggtgctgacc tacaccaagg ccggcaagca gcacggctac ttcgcgaccg
    11641 agcaggacgc cgaggtcttc gagcacgagc tcacctatgc ccttctgcac caggtgttct
    11701 cgttcaactc gccggtgtgg ttcaacgtcg gcaccccctc accgcaacag gtcagcgcct
    11761 gcttcatcct ctcggtcgac gactcgatgg acagcatcct gaactggtac aaggaggagg
    11821 ggaagatctt ccaggggggc tcgggcgccg gcttgaacct ctcgcgcatc cgctcctcca
    11881 aggagttgct ctcctccggt ggcacggcgt ccggcccggt ctcgttcatg cggggcgccg
    11941 acgcctccgc cggcaccatc aagtccggtg gcgcgacccg tcgtgccgcc aagatggtcg
    12001 tcctcgacgt cgaccacccc gacatcgtcg agttcgtcga gaccaaggcc cgcgaagagg
    12061 acaagatccg cgcactgcgt gatgctggct tcgacatgga cctcggcggc aaggacatcg
    12121 tctcggtcca gtaccagaac gccaacaact ccgtccgcgt caccgacgag ttcatgcgcg
    12181 ccgtcgaaga gggcaccgaa ttcaacctca cctcgcgcct ggacggctcc gtcgtcgagt
    12241 ccgtcgacgc ccgcgagctg atgcgcaaga tctccgaggc cgcgtgggag tgcgccgacc
    12301 cgggcatgca gtacgacgac acgatcaacg cgtggcacac caaccccgag acgggccgga
    12361 tcacggcatc taatccttgc tcggaatata tgtcgctcga caattcatcc tgcaatttgg
    12421 cgtcgctcaa tttgctcaag ttccttcgcg aggatgacac attcgacgca tccactttcc
    12481 aaaaggttgt cgaattggtc ataaccgcaa tggatatctc catttgcttc gccgacttcc
    12541 cgaccgagcc gatcggcaag accacggtcg actatcgcca gctgggcatc ggctacgcca
    12601 acctcggcgc gctgctcatg gcgaccggtc acgggtatga cagcgaaggc ggccgggcac
    12661 tcgcggccag catcacctcg ctgctgaccg gcgcggcata caagcgatcg gcggagatgg
    12721 ccggcgtcgt cggtccgtat gccgggtacg ctcgcaacgc cgacgcccac aagcgcgtga
    12781 tgcgcaagca ccaggccgcc aacgacgcag tccggacccg ccacacgatg gacgccgaca
    12841 tccacaagct cgccactagg gcctgggatg acgtgctaac gctcggggaa aggaatggct
    12901 tccgcaatgc gcaagccagc gttctcgcac cgacgggaac catcggcttc atgatggatt
    12961 gcgacacaac gggaatcgag ccagattttt cgctggtcaa attcaagaag ctcgtcggtg
    13021 gcgggtcgat gcaaatcgtc aacctgacga ttccgcgagc cctgcgcaag ctcgggtatg
    13081 ccgaggagac cgtcgaggcg atcgtcgagt tcatcgccga caagggccac gtcgtcggcg
    13141 cgcccggtct gaagaccgag cactacgagg tcttcgacac ggccatgggt gagcgggcca
    13201 tctcggcgat gggtcacgtg cggatgatgg cgtccgtcca gccgttcctg tccggcgcca
    13261 tctccaagac ggtcaacctg ccggaaagcg cgtcggtgga ggagatcgag gatgtctacg
    13321 tccagggctg gaagatgggc ctgaaggcgc tggcggtcta ccgcgacaac tgcaaggtcg
    13381 gccagccgct gtccgatgcc ggatccacgg ccaaggacaa ggaggccgcc gcggcggcgc
    13441 ccgtcgagaa ggtcgtcgaa taccgcccgg tccgcgagcg cttgcccaag cgccgcaact
    13501 cgagaaccac ctccttcgcg atcggcgggg cggagggcta tatgaccgcc tcgacgtatg
    13561 aggacggccg cctcggtgag gtcttcctca agttcggcaa gcagggctcc accctcgcgg
    13621 gggtgatgga tgctttctcg atcgcgatct ccgtggcgtt gcagtatggc gtgccgctcg
    13681 agacctgggt ggagaagttc accaacatgc gcttcgagcc ggccggcatg accgacgacc
    13741 cggacgtgcg gatgggccag tccctcatgg actacgtctt ccgccggctc gcgctcgacc
    13801 acctcgactt cgagacccgc tcctactacg gcatctacac cgccgaggag cgcgctcgcc
    13861 agctcgagac cggctcctac gcccccgtgg atgaggagtc cgaggaggac atcgaggacg
    13921 agatggagat catgcaggag ggcgtcggtg ctcaggctcc gaagaagacc gccgagccca
    13981 agcagagccc gcaccacgac aaggacctcg acgccaccaa ggtccagtcc ggctccggcg
    14041 ccgcctcggc ccgcaaggtc gacgccgacg tgcactcctc ggccgacctg ctcgccaagt
    14101 tcggcaacag caaggccgcc gacgccccga tgtgcatgac ctgcggcacc aagatgcgcc
    14161 ccgcgggctc ctgctacgtc tgcgagggct gcggctccac cagcggctgc agctgagcgc
    14221 cgctgccggg aagtaagtcc gcggtttgga cccacatacg ggtcctaacc gcggacctat
    14281 ttccggggag gagatgtcgg tgtcgcgtcg tagtgtcgtg tcagtgtcag ggtgatggca
    14341 ccgctcacgc ggtggttgga tcctgagcat ccgg
//
LOCUS       DAOPJG010000075        14311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4439_length_14311_cov_7.215699, whole genome shotgun sequence.
ACCESSION   DAOPJG010000075 DAOPJG010000000
VERSION     DAOPJG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 14311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14311
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4439_length_14311_cov_7.215699"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1286)
                     /locus_tag="PKU75_08510"
     CDS             complement(96..1286)
                     /locus_tag="PKU75_08510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HPF80957.1"
                     /translation="MTPPREPTPRFAGSRVVAGAGLVALVAALGMQCLVAARGWSLPG
                     GQHWVDLDIYLASAQRYADTGQLYCLQECRELGFTYPPGAVVPLLGLVQLPVTLVRVL
                     VTALTLSTLVAALAVIFALGRMPRPAIPWALAAVTASEPARMTVGQGQVSVVVACLAL
                     LGLALARPAAGGLLALAAATKLTPALMALPLLGHPNPRERRRLLIAIVTAATLLGVGL
                     LLGPRLTWDYLTKVLPDTSRVGALDSISNISVAGLLAHWRVGDPWPTLAAGGTGLVLL
                     AVLVSRARGRTLDDSRDRVLVAVAGGLITLAVTPVAWTHHALFAPVAACLAIQRRRTA
                     YRLAGLVCLALWMPPLMHIALDHFGRPASALVQSLRPLSLLVLFALAVADIAGTAVST
                     GERA"
     gene            complement(1364..3451)
                     /locus_tag="PKU75_08515"
     CDS             complement(1364..3451)
                     /locus_tag="PKU75_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80958.1"
                     /translation="MIVSLPAPADGIEPGSGLGAWLQRLVIGAGLLVLATAALAAPLG
                     YAGWWRPWLVLPLLVLVAGGVGWLVARLPAARVDTAPAAALALVGAAYTAWAAATHST
                     QILPRRDAGSNLQAAISLADTGYRIVPLSPAGMGAPAVLRVHDVTLGSPAFYQLGDAA
                     HPSIQPQFVIGPAAIYSPGRWLGGLSTMFVQPAVLAGLSVLGLGLIVAVLLRGWWGPV
                     AAASVALQFPLLHTARATYSEPLAELTIVAGALAWVLAARFDHRRLALLAGLLVGGTT
                     LVRIDGLREAILLIPVAALGLIQRRSWPAPLVRGTAIGALGGGAAAQALSYRYLGDIA
                     GSLLPLIALGAFMALVSWALVVLSRRGAGLPDSVRRLLPAACGLGVLVIGLLLALRPL
                     FMTVRQNPNDPGANVVAGLQLRQGLTIDGARTYAEQSVAWLSWWVGPVALVIALLVAA
                     ALAARLGRAWTTRPQIPGRPPALLPTWAGPYVVALGSTLLTLWRPGITPDHPWAERRL
                     LIALPFVAVLVVVGAAWVWRGAATSLRSQPARAGLALLTLASLVIPTALATWPHRSER
                     VEAGSQARVAALCRALQPGDIVLSVDDRAANEWPPVVRGMCNKPALVAKYYARRHPEA
                     LRILVDGVVDAAPPGSRVVLLAGESPQALARLGFTPQPVASGEVLEDERLLERRPDGL
                     VPLRIDLWLAPAS"
     gene            3497..4168
                     /locus_tag="PKU75_08520"
     CDS             3497..4168
                     /locus_tag="PKU75_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HPF80959.1"
                     /translation="MRVDQLLPAMLRSDPARPRITCYDDTTGERIELSGKVLANWVAK
                     AQGMLNEQFDAAPGMVLRLDLPPHWRTVYWALAAWGLGMTIDLTTDHADATVTTNPLV
                     EGVVVTLPALARSAPTPLTPGAIDEARELAAYGDQFTPWDSAEDDTAALAAAGATRQR
                     YADPPAYPLSAGARVHTATRDVGEFLQLAIGAYAADGSLVLSIGPDPARLPGRLRTEG
                     VSESR"
     gene            complement(4152..5279)
                     /locus_tag="PKU75_08525"
     CDS             complement(4152..5279)
                     /locus_tag="PKU75_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPF80960.1"
                     /translation="MSTATRRLIAPAVVTALAVPTIAAAAVPPPPKPPVTLPSAVDVS
                     MPYEGQGFCELVTRPGVADFARTVSTHYSRTDYHTVRTCLGDVSEHYDGRALDWMLSA
                     YDPKDKAIADSVTKWLTQPVNGVYGANARRLGIMYIIWNGKTWKSYRNPETWTTYTGA
                     VPHTDHIHFSFAWDGACKRTSWWTGKALTTVSMAKCGSSNGPLVSVETEFTQYKDTLL
                     MLGSKGDAVVLAQQNLGVPADGDFGPITRSAVINFQKKWKLDVTGRVNKGVWNQMERL
                     QYPLIKYRSTTVLRKGMSGGQAIKDAQRALRITQDGIFGSGMEAAVKRIQGKYRLAQT
                     GVIRPLTWAALDEELRSRMRQAEFDAGIDKAAAEVLLSAIR"
     gene            5441..6250
                     /locus_tag="PKU75_08530"
     CDS             5441..6250
                     /locus_tag="PKU75_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPF80961.1"
                     /translation="MDKVVTSPADAVADIPSGATLAVGGFGLCGIPSVLIQALHDSGV
                     DGLTAVSNNCGVDDWGLGILLRDKRIATMIGSYVGENKEFERQYLSGELEVRLTPQGT
                     LAEKLRAGGAGIPGFYTVTGVGTQIAEGGLPWRYAADGTVALSSPAKETKEFTVGGVT
                     ATYVLEESIVTDFALVRAWKGDRHGNLIFRSSARNFNPLAAMAGRTTIAEVEELYEPG
                     ELDPDQVHLPSIYVQRVVPLTPEQADEKRIERRTTRPRTTAAPTMSTGLGG"
     gene            6255..6890
                     /locus_tag="PKU75_08535"
     CDS             6255..6890
                     /locus_tag="PKU75_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HPF80962.1"
                     /translation="MPLTREEMAARAAAELKDGDYVNLGIGLPTLVPNYVADDIEIVL
                     QSENGILGVGAYPYEGDEDPDLINAGKETVTLRKGASIFDSATSFGMIRGGKVDAAIL
                     GAMQVSEAGDIANWMIPGKMVKGMGGAMDLVHGAKHVIVLMEHTAKDGSHKIVQECSL
                     PLTGKRVVQRIITDLCVMDVTADGLVLRELAPGVTEEQVREATGPDFTVAL"
     gene            7034..8020
                     /locus_tag="PKU75_08540"
     CDS             7034..8020
                     /locus_tag="PKU75_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80963.1"
                     /translation="MKEPVPRTSRQEVALRLVRPYGGICTRSMLVDTGISQGAIDAEI
                     RARRWLRIGRHTVAIPEAMAPSWPSDASSWPRARPGAAGTPELSQLPLLWWAVWESGT
                     GAVLDGASSLTASGLTGYTQDRIDVSVEAGSRTIRHPGIRIKERRPLGPLAPGGIPRV
                     ETALSTIRAAQLAVSDRQAALVICMSVQQRIVEPDRLLRRWRGIARSKRRSLIGPVIG
                     DVCDGAHSLGELDFAALCRARDLPEPVRQQLRQGPNGRIYLDVAFACGLVVEIDGAGH
                     SWGLAPVDDALRANHVTLQNRSVLRIPVIGLRVMPQAFMDQVATGIRLRSPH"
     gene            complement(8087..9112)
                     /locus_tag="PKU75_08545"
     CDS             complement(8087..9112)
                     /locus_tag="PKU75_08545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF80964.1"
                     /translation="MPAPHPATTPWPVVSVIVPVLNEERHLADAVAMIAGQDYPGEME
                     IVLALGPSRDATDAVAAGLAAADPRIRLVPNPTGRTPAGLNAAIAASYGEIVARVDGH
                     AEIPADYLRTAVTALQEQGADNVGGVMHAEGRTPFERAVATAMRSRIGVGNAAFHVGG
                     QAGEAETVYLGVFRRAALEAVGGFDEHYERAQDWEMNHRIRGRGGKIWFTPDLKVTYR
                     PRSTPKALARQYFQYGRWRHVIARHHQGSINLRYLAPPAMVTGTTAATLIGLVWKPAL
                     LVPAAYAGAVAAGGVLAARGESPPVKALTPVALAVMHWSWGVGYLTSPRHLAAGRIND
                     PRTGGSL"
     gene            9277..10710
                     /locus_tag="PKU75_08550"
     CDS             9277..10710
                     /locus_tag="PKU75_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPF80965.1"
                     /translation="MTTSPPGGRDTSGDAQAIPQRRRTPDGSAPAHHPAPGHERSDAT
                     RPVPTRGADPYYGWHEEHWDEEEWREGRFSGASAAAPTTPVAQPARQLNQPAAARRPR
                     PAAPATTPIPTTRPAPARAAEQYAGGRAAYDPPPQAPTGPPARGPRMPAPPRPPRRRH
                     TFRRIVAAVVLLGLLWVGGTAWAVKSTWSAVGRVKSTPAAGHIADTEGRNYLMVGSDG
                     RTDLTAKERNELGTGWAEGARTDSIMVLHTGGGDPTLLSIPRDSYVEIPGHGMNKINA
                     AFNTGGPPLLAETVEKVTGLHLDGYIEIGFGGFAKLVDSVGGVRMCLPNAINDEKAHI
                     DLPAGCQVLDGKNALGYVRMRYSDPEGDLGRVKRQRAFLGALMGKLATPTNFALPWKL
                     HSVGTSGASAITIGEGDSMTSTARAFLGLRAVAGGKGQSVTVPVANPGVPTAVGEVVE
                     WDAAKAQALFTALQNDDDLPADLIPAK"
     gene            complement(10858..13452)
                     /locus_tag="PKU75_08555"
     CDS             complement(10858..13452)
                     /locus_tag="PKU75_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HPF80966.1"
                     /translation="MFVHRRRTILAAVVAAPIALAGLPAVAGPRVVAHALPVAHALPA
                     ATSSAAAPAAPEQHTDLAVPVAGATAAEAALAYARADHRGTGDLNVLSEDTDDIGSVV
                     RLAQSVDGIPVLGGQIIVRLTGNSGSYVVRGTTDGTAAVTGSRTPTWSAAALERVVTH
                     AAEKRFPGERVSAKAGDLVILPFGAGTLTRHVTVTLARTGGVHDVYVGARTGAVLLAV
                     DRHKTVDVISTERNLAGQTVKVHADKAGGLFTLTDTTRKPYGPAIQAQYLTEIGEQPV
                     EATRLPFGADDTERGTIDAHLDVAKAADYYRNVLGRNSIDGHGGDLIHVTGMNMVNAF
                     WDGTQMVIGLGDAEYKPLSSDVDVVAHEMSHGVIDHTAGFIYQSQSGALSEAYSDYFG
                     NAVDVGQSASAMTAPRNGLIGEDLCRTLSPIKCAIRNLDDGRTVNDYTGTLADIGGVH
                     LNATIYGGALWDIRRAMRPSLSDRVVFRGLFAYMTPWSTFTDGRDATLAAAADLGLTA
                     AQRAAITKAFDSRGIVRGWERDGRTDADRTLLSDLPFFTDWYPPVAGGSRWALADIDM
                     YGNWPTAFKTGRTDGTGTERVALPDGFTLGDLAVDGRYVAATGLTADEIGVWLYDTVT
                     KKGTWLARSDASHFYVYPTVAGGRVAWQDSATVNDKPDISVYVKDLATNVVRELDKPG
                     DVAVHFPSLSRTTLVHVQGPMTAKDLVVRDAATLKVIATKRIVTGDVDTTFIPTALTS
                     QVVWSYVNGDREWPLGPEVTEFVSPRNLSSKRILNRLDLPTLWPANLIAQATETAFTQ
                     IRPKSLLGASPEVIQKPLAGGNAVPVTCDKGGAYLFAADQKQAVVWLSGAQGKVDLVM
                     RSTPAGPC"
     gene            13774..>14311
                     /locus_tag="PKU75_08560"
     CDS             13774..>14311
                     /locus_tag="PKU75_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPF80967.1"
                     /translation="MTRSAILMRPAPACCPTIPVPAPARCRRRLGFVDGRKRIVLDDF
                     VACGGAKAMDIPGHVTALDTDTDLVTVSVGGNDISWIEAVATCILADEATCKGAVNRS
                     RTAVVQSLPGLLDTVYSSIDRAAPNARVLVTGYARLFSPEYGDYVTPYGTVTAAEQQL
                     MNDGADLLNSVIAARAAAH"
BASE COUNT         2160 a         5005 c         5167 g         1979 t
ORIGIN      
        1 catcccgatg tcggcataca gcacgaacgc cacccccgcc cacgcgagcg cccacaacac
       61 cccccgcacc cgcaacgacc ccggagccgg cgccgtcacg cccgctcccc cgtcgagaca
      121 gcagttccgg cgatgtcggc gaccgccagg gcgaagagga cgagcaggct gagcgggcgc
      181 aggctctgca cgagcgccga ggcggggcgg ccgaagtggt cgagggcgat gtgcatcagc
      241 gggggcatcc acagggcgag gcagaccaga cccgccagcc ggtatgcggt gcggcggcgt
      301 tggatggcca ggcaggcggc gacgggggcg aagagggcgt ggtgggtcca ggcgaccgga
      361 gtcaccgcga gcgtgatcag gccgccggca acggcgacca ggacccgatc gcgactgtcg
      421 tcgagggtgc ggccgcgggc ccggctcacg aggacggcga gcaggaccag cccggtgccg
      481 ccggcggcga gggtcggcca ggggtcgccg acccgccagt gggcgagcag gccggcgacg
      541 gagatattgg agatgctgtc gagcgcgccg acgcgggagg tgtccgggag gactttcgtc
      601 aggtagtccc aggtcaggcg ggggccgagc aggagcccga cgccgagcag ggtggctgcg
      661 gtgacgatcg cgatcagcag tcggcgccgt tcgcgcgggt tggggtggcc gagcagcggc
      721 agggccatca gcgcgggggt gagcttcgtg gccgcggcga gcgcgagcag cccgccggcg
      781 gcgggccgcg ccagggcgag gccgagcagg gcgaggcacg ccacgacgac gctgacctgg
      841 ccctggccga cggtcatccg cgccggctcg ctcgccgtca ccgcggcgag cgcccacggg
      901 atcgccggcc gcggcatacg acccagggcg aagatcacgg ccagcgccgc gacgagcgtg
      961 ctcagcgtca gggcggtcac gagaacccgc acgagggtga cgggcagctg gacgagtccg
     1021 agcagcggca ccaccgcccc cggcgggtag gtgaacccca gctcgcggca ctcctgcagg
     1081 caatagagct gcccggtgtc ggcataccgc tgggcggagg cgaggtagat gtccagatcg
     1141 acccagtgct gcccgccggg cagcgaccac ccgcgcgccg cgacgagaca ctgcataccc
     1201 aacgcggcca ccagcgcgac caggcccgcc cccgcgacga cacggctccc ggcgaacctg
     1261 ggcgtcggtt cgcgcggcgg ggtcattcct tcaatggtag gcgcggcccg ggaacaccgc
     1321 ccaacccgca ccacgtgacc ggggacaggc ccccagccgc ccgtcaggag gcgggggcga
     1381 gccacaggtc gatgcgcagc gggaccaggc catcggggcg gcgttcgagg agacgctcat
     1441 cctcgagcac ctcgccgctg gcgaccggtt ggggggtgaa gcccagtcgg gccagggcct
     1501 gcggcgactc gcccgccagc agcaccaccc gcgatccggg cggtgcggcg tcaaccaccc
     1561 cgtcgacgag gatgcgcagc gcctcggggt ggcgacgcgc gtagtacttc gcgacaaggg
     1621 cgggcttgtt gcacatgccg cgcacgaccg gcggccactc attagccgcc cggtcatcga
     1681 cgctcaggac gatatcgccg ggttgcaagg cccggcagag cgctgctact cgcgcctggg
     1741 agccggcctc gacccgctcg ctccggtgcg gccaggtcgc cagcgcggtg ggtatgacga
     1801 ggctcgccaa ggtcaggagg gccagacctg ctcgggccgg ttggctgcgc aggcttgtcg
     1861 cggcgccgcg ccacacccac gcagcaccga ccacaaccag gacggcaacg aagggcaagg
     1921 cgatcaggag acgacgctcg gcccacggat gatccggtgt gatgcccggg cgccacaagg
     1981 tgagcagcgt cgaacccagg gccacgacat acgggccggc ccaggtgggc agcagggccg
     2041 gcggccgacc gggaatctgc ggccgtgttg tccacgcgcg acccaaccgg gcagcgagcg
     2101 cggccgcgac gagaagcgcg atgacgaggg cgactggccc gacccaccag gacagccacg
     2161 ccaccgactg ctcggcgtag gtgcgggcgc catcgatggt cagcccttgt cgcagttgca
     2221 gcccggcgac gacattcgcg ccggggtcgt tcgggttctg ccggacggtc atgaacaggg
     2281 ggcggagcgc gagcagtagc ccgatcacga ggacacccag tccgcatgcc gcgggtagga
     2341 gccggcgcac cgagtccggc agtcccgcgc cgcggcggga gaggacgacg agcgcccagc
     2401 tcaccagcgc catgaacgcc ccgagggcga tcaggggcag caggctgccg gcgatgtccc
     2461 cgagatagcg gtaggacagt gcctgcgcgg cggccccgcc gccgagcgct ccgattgcgg
     2521 ttccgcgcac cagcggggcg ggccagctgc gccgctggat caggcccagc gccgcgaccg
     2581 ggatgagcaa gatggcctcg cgcagcccgt cgatccgcac cagggtcgtg ccgccgacga
     2641 gcaagccggc gagcagcgcc aggcgccggt ggtcgaaccg agctgccagc acccacgcaa
     2701 gagcccccgc gacgatggtc agttcggcga ggggctccga atacgtcgct cgggccgtat
     2761 gcagcagcgg gaactgcagg gctaccgagg ccgcggcgac cggcccccac cagccgcgca
     2821 gcagcaccgc gacgatcaac ccgaggccca agacactcag cccggccagc accgccggtt
     2881 gcacgaacat cgtcgagagg ccgccgagcc agcggccggg cgaatagatg gcggccgggc
     2941 cgatgacgaa ctgcggctgg atcgacgggt gagcggcatc gcccagctga tagaaggccg
     3001 ggctccccag cgtgacgtcg tgcacccgca ggacggccgg cgcccccatg cccgctggcg
     3061 acagtgggac gatgcgatat ccggtgtccg ccagcgagat cgcggcctgc aggttcgagc
     3121 cggcgtcgcg gcggggcagg atctgggtac tgtgcgtggc cgccgcccac gccgtgtatg
     3181 ccgcccccac gagggcgagc gccgccgccg gagcggtgtc cacgcgggcg gccggcagcc
     3241 gcgcgacgag ccaccccacg ccgcccgcca cgagcacaag cagcggcagg acgagccagg
     3301 ggcgccacca cccggcatac cccaacggcg cggcgagtgc agccgtcgcc agcacgagca
     3361 gcccggcccc gatcaccagg cgttgtagcc aggccccgag cccgctgccc ggttcgatgc
     3421 cgtcggcggg agcgggcagg gagacgatca cgagcgtcga ggctacctgt cggcccgact
     3481 ctttagagtg agccccatgc gtgtcgacca gctgctcccc gcgatgctcc gcagcgatcc
     3541 ggcgcgcccc cggatcacct gctacgacga cacgaccggt gagcggatcg agctgtccgg
     3601 caaggtgctg gccaactggg tggccaaggc gcagggcatg ctgaacgagc agttcgacgc
     3661 cgcgcctggc atggtgttgc ggctggatct gccgccgcat tggcgcaccg tctactgggc
     3721 tctcgccgcc tgggggctgg ggatgacgat cgatctcacc acggaccacg cggacgcgac
     3781 cgtcacgacc aacccgctgg tggagggcgt cgtcgtcacc ctccccgcgc tggcccggtc
     3841 cgcccccacc cccctgacgc ccggcgcgat cgacgaggcg cgggagttgg cggcatacgg
     3901 cgatcagttc acgccgtggg actccgccga ggacgacacc gcggccctcg ccgccgccgg
     3961 ggcgacccgg cagcggtatg ccgacccgcc ggcatacccc ctgagcgcgg gagcgcgggt
     4021 gcacacggcg acgcgcgacg tcggcgagtt cctgcagctg gcgatcgggg cctacgccgc
     4081 cgacggctcg ctcgtgctga gcatcggccc cgacccggcc cgcctgcccg ggcggctgcg
     4141 gaccgagggc gtcagcgaat cgcgctgagc aggacctcgg ccgccgcctt gtcgatcccg
     4201 gcgtcgaact cggcctgccg catccggctg cgcaactcct cgtcgagagc ggcccaggtc
     4261 agcgggcgga tgacgccggt ctgggcgagg cggtacttgc cttggatgcg cttgaccgcg
     4321 gcctccatgc cggacccgaa gatgccgtcc tgggtgatcc gcagcgcccg ctgggcgtcc
     4381 ttgatcgcct gaccgcccga catgcccttg cgcaagacgg tggtcgagcg gtacttgatc
     4441 agcgggtatt gcaggcgttc catctggttc cagacgccct tgttgacccg gccggtgacg
     4501 tcgagcttcc acttcttctg gaagttgatc acggcgctgc gagtgatggg gccgaaatcc
     4561 ccgtcggcgg gcaccccgag gttctgctgc gcgaggacga cggcgtcgcc cttggagccg
     4621 agcatcaaca gggtgtcctt gtactgcgtg aactccgtct ccacgctgac gagcggcccg
     4681 ttcgaggagc cgcacttggc catgctcacg gtggtcagcg ccttgccggt ccaccagctg
     4741 gtgcgcttgc acgcgccgtc ccaggcgaag ctgaaatgga tgtggtcggt gtgcggcacg
     4801 gccccggtgt aggtcgtcca cgtctccggg ttgcggtagg acttccaggt cttgccgttc
     4861 cagatgatgt acatgatgcc aaggcgtcgg gcgttggcgc cgtagacccc gttgaccggc
     4921 tgggtcagcc atttcgtgac cgagtcggcg atcgccttgt ccttcgggtc gtatgccgag
     4981 agcatccaat ccagggcccg cccgtcgtag tgctcggaga cgtcgccgag gcaggtgcgc
     5041 accgtgtggt agtcggtgcg gctgtagtgg gtggagaccg tcctggcgaa gtcggccacg
     5101 ccggggcggg tcaccagctc gcagaagccc tgcccctcat agggcatcga gacgtcgacg
     5161 gcgctgggca gcgtgaccgg gggcttcggc ggcggcggaa cggcggccgc ggcgatcgtc
     5221 ggcacggcga gcgccgtcac gacggcgggc gcgatgagtc ggcgggtggc tgtggacatg
     5281 gggtgaactc ctgctgtgct gccagcgccg gggcactgcc ccgcgggggt cggacggtat
     5341 gccgaccgca tcagttgtcc cacgagtcac acccgaaagg caagcctcac acgcggatcc
     5401 acaggtgccg ggcccgatgc gcctcgatag ggtgaggggt atggacaaag tcgtcaccag
     5461 ccccgccgac gcggtggccg acatccccag cggcgcaacg ctcgcggtgg gcggattcgg
     5521 cctgtgcggc atacccagcg ttctgatcca ggccctgcac gacagcggcg tggacggcct
     5581 caccgccgtc tccaacaact gcggcgtcga cgactggggt ctgggaattc tgttgcgcga
     5641 caagcgaatc gccacgatga tcggctcgta tgtcggggag aacaaggagt tcgagcggca
     5701 atacctctcc ggggaactcg aggtccgcct gaccccccag ggcaccctgg cggagaaact
     5761 gcgggccggg ggcgccggca tacccggctt ctacaccgtg accggcgtgg gcacccagat
     5821 cgccgagggc gggctgccct ggcggtatgc cgcggacggc accgtcgcgc tgtcctcgcc
     5881 ggcgaaggag accaaggagt tcaccgtcgg cggcgtcacg gccacctatg tcctcgagga
     5941 gtcgatcgtc accgacttcg cgctggtgcg cgcgtggaag ggcgaccggc acggcaacct
     6001 gatcttccgc tccagcgccc gcaacttcaa tccgctcgcg gcgatggccg ggcgcaccac
     6061 gatcgccgag gtcgaggagc tgtacgagcc cggcgaactc gatcccgacc aggtccacct
     6121 gccgagcatc tacgtccagc gcgtcgtgcc gctgacgccc gagcaggccg acgagaagcg
     6181 gatcgagcgg cggacgaccc ggccgcgcac gaccgcggca cccaccatga gcaccggact
     6241 cggaggctga tcccatgccc ctcacccgtg aagagatggc cgcccgggcc gccgccgaac
     6301 tcaaggacgg cgactatgtc aacctcggga tcggcctgcc gaccctggtg cccaattacg
     6361 tcgccgacga catcgagatc gtcctgcagt ccgagaacgg catcctcggc gtcggtgcct
     6421 atccctacga gggcgatgag gatccggacc tgatcaacgc cggcaaggag acggtgaccc
     6481 tgcgcaaggg cgcgagcatc ttcgactccg cgaccagctt cgggatgatc cgcggcggca
     6541 aggtcgacgc cgccatcctc ggcgccatgc aggtctccga ggccggtgac atcgccaact
     6601 ggatgatccc cggcaagatg gtcaagggca tgggcggggc gatggacctc gtccacggcg
     6661 ccaagcacgt catcgtcctg atggagcaca ccgccaagga cggctcgcac aagatcgtcc
     6721 aggagtgttc gctgcccctg accggcaagc gggtcgtcca gcgcatcatc accgacctgt
     6781 gcgtcatgga cgtcaccgct gacggcctcg tcctgcgcga actcgccccc ggtgtcaccg
     6841 aggagcaggt ccgcgaggcc accggccccg acttcaccgt cgccctctga gtcgctaagt
     6901 ccgacgtttg acgtgctatc gcacgtcaaa cgtcggactt agcgtgtcca cagggctcgg
     6961 gcctggttcg ggttgtccac aggctttgcg gcaacgggtg cggccacgcc caggtcgccg
     7021 gtctactcga catatgaagg aaccggtccc ccgcacgtcg cgccaggagg tcgccctccg
     7081 gctcgtgcgg ccgtacggcg ggatctgtac gcggtccatg ctcgtcgaca cgggcatctc
     7141 ccaaggtgcc atcgacgccg aaatccgggc cagacgatgg ctgcgcatcg gacggcatac
     7201 cgtcgcgatc cccgaggcaa tggcgcccag ttggccgtcg gacgcgtcgt cgtggccccg
     7261 ggctcggccc ggcgcagcgg gcacgcccga gctctcgcag ctgccgttgc tctggtgggc
     7321 ggtatgggag tcgggcaccg gggccgttct cgacggtgcc agttcactga cggcgtcggg
     7381 gctgaccggc tacacgcaag accgcatcga cgtctcggtc gaggccggct ctcgaaccat
     7441 tcggcatccg ggaatccgca tcaaggagcg gcgcccgctg gggccgcttg cgcccggagg
     7501 gatcccgcgc gtcgagaccg ctctgtccac gatccgcgcg gcccaattgg ccgtcagcga
     7561 tcggcaggct gccctggtca tctgtatgtc ggtgcaacag cgcatcgtcg aaccggaccg
     7621 gctgttgcgt cggtggcgtg gaatcgccag gtcgaagcgg cgcagcctga tcggcccggt
     7681 gatcggggac gtgtgcgacg gagcgcattc cctcggtgaa ctcgacttcg ccgctctctg
     7741 ccgggcccgc gacctgcccg aaccggttcg ccagcaactc cggcaggggc ccaacggccg
     7801 catctatctc gacgtcgcct tcgcctgcgg cctcgtggtg gagatcgacg gcgctggcca
     7861 ctcctggggg ctggccccgg tcgacgatgc cttgcgcgcg aaccacgtga ctctgcagaa
     7921 ccgcagcgtg ctccgcatcc ccgtcatcgg tctgcgcgtc atgccgcagg cgttcatgga
     7981 ccaggtggcg acgggaatcc gcctccgctc cccccattaa gtccgacgtt tgacgagcga
     8041 tagcacgtca aacgtcggac ttttgggggg tcggggggcg gagtcgtcag agggagccgc
     8101 cggtgcgggg atcgttgatg cggccggcgg cgaggtggcg cggggaggtg agatagccca
     8161 cgccccacga ccagtgcatg acggccagcg cgacgggggt cagggccttg acgggcggag
     8221 actccccgcg cgccgcgagg acgccgccgg cggcgacggc accggcatac gccgcgggca
     8281 ccagcagggc cggcttccac acgaggccga tcagcgtggc ggcggtggtg ccggtcacca
     8341 tcgccggcgg cgccaggtag cgcaggttga tcgagccctg gtggtggcgg gcgatgacgt
     8401 gccgccaccg gccgtactga aagtattgcc gcgcaagggc tttcggggtg ctccgcggtc
     8461 gataggtgac cttcagatcc ggcgtgaacc agatcttgcc gccgcgcccg cggatgcggt
     8521 ggttcatctc ccagtcctgg gcccgctcgt agtgctcgtc gaagccgccg acggcctcca
     8581 gcgcggcccg ccggaagacc ccgagataga ccgtctcggc ctccccggcc tgcccgccga
     8641 cgtggaaagc ggcgttgccc acgccgatgc ggctgcgcat ggcggtggcg acggcgcgct
     8701 cgaacggggt gcgcccctcg gcgtgcatga ccccgccgac gttgtcggcg ccttgttcct
     8761 gcagcgccgt cacagcggtg cgcaggtagt ccgccgggat ctcggcgtgc ccgtcgacgc
     8821 gtgccacgat ctcgccgtat gaggccgcga tagccgcgtt caagccggcc ggtgtgcggc
     8881 cggtcgggtt gggcaccaag cgaattcgcg ggtctgcggc ggccagcccg gcggcgacgg
     8941 cgtcggttgc gtcccgcgac ggcccgagcg ccaggacgat ctccatctct cccgggtagt
     9001 cctgtccggc gatcatcgcc acggcatcgg cgaggtgccg ctcctcgttg agcaccggca
     9061 cgatcaccga gacgacgggc cacggcgtgg tggcggggtg gggagcaggc acgccctcca
     9121 gtatcgcctc cggacaccgc aaccccggcc aaccccgccc tcgacacccg ggctcgcacg
     9181 acccagcccg gccgggaggg cggcccgcgg cgcgccacgc cccgggcggc gggggtcggg
     9241 gggcatgtcc agcgcggccg attacccttg cctgccatga cgacctctcc tcccggcggg
     9301 cgcgacacct ctggggacgc gcaggcgatc ccgcagcggc gccgcacgcc cgacgggagc
     9361 gcgccagcgc accatccggc gcctggtcac gagcggtccg acgccacacg cccggtgccg
     9421 acccgcggcg ccgaccccta ttacggctgg cacgaggagc actgggacga ggaggagtgg
     9481 cgcgagggcc gcttctcggg cgcgtccgcg gcggcgccga ccacgccggt ggcgcagccg
     9541 gctcggcagc tgaaccaacc cgccgccgcg cgtcgcccgc gcccggccgc cccggcgacg
     9601 accccgatcc cgacgactcg ccccgcccct gcccgggccg cggagcagta tgccgggggg
     9661 cgggcggcat acgatccgcc gccgcaggcg ccgaccgggc cccccgcgag gggtccgcgg
     9721 atgccggcac caccccggcc gccgcgacgg cggcatacct tccggcgcat cgtcgccgcg
     9781 gtcgtcctcc tcggcttgtt gtgggtcggc ggcaccgcgt gggcggtcaa gtcgacctgg
     9841 tcggcggtcg gccgcgtgaa gtccacgccc gcggcaggtc atatcgccga caccgagggc
     9901 cgcaactacc tgatggtcgg ctccgacggc cgcaccgacc tgactgcgaa ggaacgcaac
     9961 gaactcggca ccggctgggc cgagggcgcc cggaccgact cgatcatggt gttgcacacc
    10021 ggcggcggcg accccacgct gttgtcgatc ccccgcgact cctacgtcga gatccccggg
    10081 cacgggatga acaagatcaa tgccgccttc aacaccggcg gcccgccgct gctcgccgaa
    10141 accgtcgaga aggtcaccgg gctgcacctg gacggctata tcgagatcgg gttcggcggc
    10201 ttcgccaaac tcgtcgactc cgtcggcggg gtgcggatgt gcctgcccaa cgcgatcaac
    10261 gacgagaagg cgcacatcga cctgccggcc gggtgccagg tcctcgacgg caagaacgcc
    10321 ttggggtatg tgcggatgcg ctattccgat cccgagggcg atctcgggcg ggtcaagcgg
    10381 cagcgcgcct tcctcggtgc gctgatgggc aaactggcga cccccaccaa tttcgcgttg
    10441 ccgtggaagc tgcactcggt cggaacctcc ggcgcctcgg cgatcaccat cggtgaaggc
    10501 gacagtatga cgagcaccgc ccgcgccttc ctcggcctgc gcgccgtggc cgggggcaag
    10561 ggccagtcgg tgacggtgcc cgtcgccaac cccggagtcc ccaccgcggt cggggaggtc
    10621 gtcgagtggg acgccgccaa ggcgcaagcg ttgttcacgg ccctgcagaa cgacgacgac
    10681 ctccccgccg acctgatccc ggccaagtag cgattcggct ctcgagggct gacgaggtgg
    10741 tgcggcgccc tggggagagg cagggcgccg caccgatggg gggcgtcggg agaatctcgt
    10801 ggggtcaccc gtggggtcac ccgttccggg gaccgcgagc cgagttcgcc gggtgcttca
    10861 gcaggggccg gcgggcgtgc tgcgcatgac gaggtcgacc ttgccctgtg ccccgctgag
    10921 ccagacgacc gcctgcttct gatcggccgc gaagaggtac gcgccgccct tgtcgcacgt
    10981 caccggcacg gcgttgccgc cggccagcgg cttctggatg acctcggggc tcgcgccgag
    11041 caacgacttg ggtcggatct gggtgaaggc cgtctcggtt gcctgggcga tcaggttcgc
    11101 cggccacagc gtcggcaggt caagacggtt gaggatccgc ttgctgctga ggttgcgggg
    11161 gctgacgaac tcggtcacct ccggccccag gggccactcg cggtccccgt tgacatagga
    11221 ccagacgacc tgactcgtca gggcggtcgg gatgaaggtg gtgtcgacgt ccccggtgac
    11281 gatgcgcttc gtggcgatga ccttgagggt tgccgcgtcc cgcacgacca ggtccttcgc
    11341 ggtcatcggc ccctgcacgt ggaccaaggt ggttcggctc aggctgggga agtggacggc
    11401 cacgtcaccg ggcttgtcga gttcgcggac cacgttcgtg gccaggtcct tgacatagac
    11461 cgagatgtcc ggcttgtcgt tcacggtcgc cgagtcttgc cacgcgacgc gtccacctgc
    11521 caccgtcgga tagacgtaga agtgggaggc atcgctccgg gccagccagg tgcccttctt
    11581 cgtgacggtg tcatagagcc acacgccgat ctcgtccgcg gtcagacccg tggcggcgac
    11641 atatcgtccg tcgacggcga ggtcaccgag agtgaaacca tcgggcagcg cgacgcgctc
    11701 ggtgccggtg ccgtcggtgc gcccggtctt gaacgccgtc ggccagttgc cgtacatgtc
    11761 gatgtcggcg agcgcccagc ggctcccacc cgcgaccggc gggtaccagt cggtgaagaa
    11821 aggcagatcg gacagcaggg tgcggtcggc gtcggtccgg ccgtcgcgct cccagccgcg
    11881 cacgatgcca cgcgagtcga aggccttggt gatcgcggcc cgctgggctg ctgtcaggcc
    11941 gagatcggcg gctgcggcga gcgtggcgtc ccggccgtcg gtgaaggtcg accagggggt
    12001 catataggcg aacaaacccc ggaagacaac ccggtcggac agggacggcc gcatcgctcg
    12061 gcgaatgtcc cagagcgcac cgccgtagat ggtcgcgttg aggtgtaccc cgccgatgtc
    12121 ggccagggtg cccgtgtagt cgttgaccgt gcggccgtcg tcgagattgc ggatggcgca
    12181 cttgatcggc gagagcgtcc ggcacaggtc ctcaccgatc agcccgttcc ggggagccgt
    12241 catggcgctg gccgattgcc cgacatcgac cgcattgccg aagtagtcgg aatacgcctc
    12301 gctcagcgca ccgctctggc tttggtagat gaagccggcc gtgtgatcga tgaccccatg
    12361 gctcatctcg tgggccacga cgtcgacgtc ggaggacagc ggcttgtact cggcgtcccc
    12421 gaggccgatg accatctggg tgccgtccca gaaggcgttg accatgttca tgccggtgac
    12481 atggatcaga tcgccgccgt ggccgtcgat actgttgcgt cccaggacat tccggtagta
    12541 gtcggcggcc ttggccacgt ccagatgcgc gtcgatggtg ccgcgttcgg tgtcgtcggc
    12601 gccgaacggc aaccgggtcg cctccaccgg ctgctcgccg atctcggtga ggtactgcgc
    12661 ctggatcgcc ggaccgtagg gcttgcgcgt ggtgtcagtc agggtgaaca gccccccggc
    12721 cttgtcggca tggaccttca cggtctgccc cgcgaggttg cgttcggtcg agatgacgtc
    12781 gacggtcttg tgccggtcga cggcgagcag gacggcgccg gtgcgggcac cgacatagac
    12841 gtcgtggacg ccgccggtgc gcgcgagcgt gacggtgacg tgccgggtca gggtcccggc
    12901 tccgaagggc aggatgacca ggtcgccggc cttggcgctg acccgctccc ccgggaagcg
    12961 cttctcggcc gcgtgggtga ccacgcgttc cagggcggcg gcgctccagg tgggcgtgcg
    13021 cgagccggtc accgcggcgg tgccatcggt ggtgccgcgc acgacatacg atccggagtt
    13081 gccggtcagg cggacgatga tctggccgcc gaggacgggt atgccgtcca cgctctgggc
    13141 gagccgcacc accgagccga tgtcgtcggt gtcctcggac aggacgttca ggtctccggt
    13201 gccgcgatgg tcggcgcggg cataggccaa ggccgcctcc gcggcggtgg cccccgcgac
    13261 cgggacggcc aggtcggtgt gctgctcggg cgcggcggga gcggcggcgc tgctggtggc
    13321 ggcgggcagt gcgtgtgcga cgggcagtgc gtgtgcgacg acccgtgggc cggcgacggc
    13381 cggaaggccc gccagggcga tcggtgcggc aacgacggcc gcgaggatgg tgcgacgacg
    13441 atgaacgaac atctaggact ccccttcgag tggaccgctt cgggaccgga cgcggcagat
    13501 cgcgaccgcg ctcccaccac tccagacgcc ggaagtcgcg cgcgggttgt gccctggcga
    13561 agatctggag aactgtgtcg cgctcctggc cgtgatctgc gggaattccc cgaaacgctt
    13621 cccgccggat ccgcgcaggg ctaccgtgcg ggatggcttt gaccccgcgc gccggcagat
    13681 cgatcggctc atttgtcacc ctcgccgcgg ccgtggcaat cctcgtcccc gccgccgcac
    13741 ccgcgggcgc cgccgcaccg gcgccgatcg tctatgacgc gctcggcgat tcttatgcgt
    13801 ccggcgccgg cgtgctgccc tacgatcccg gtccctgcgc ccgctcggtg tcggcgccgg
    13861 ctcggcttcg tggatggccg caagcgcatc gtgctggatg atttcgtcgc gtgcggcggg
    13921 gccaaggcca tggacattcc tgggcacgtc accgcgctgg acacggacac cgacctggtc
    13981 acggtctccg tcggcggcaa cgacatctcc tggatcgagg cggtcgccac gtgcatcctc
    14041 gcggacgagg cgacctgtaa aggagccgta aaccgttcgc gcacagcggt tgtccagtcg
    14101 ctgcccggtc tgctcgacac ggtatacagc agcatcgacc gggccgcgcc gaacgcgcgc
    14161 gtgctggtga cgggctatgc gcggctgttc tcgccggaat acggcgacta tgtcacgccc
    14221 tacggcacgg tcaccgctgc cgagcagcag ctgatgaacg acggggcgga cctgctcaac
    14281 tccgtcatcg ccgcccgcgc cgcggcccac g
//
LOCUS       DAOPJG010000076        14087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4537_length_14087_cov_8.090080, whole genome shotgun sequence.
ACCESSION   DAOPJG010000076 DAOPJG010000000
VERSION     DAOPJG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 14087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14087
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4537_length_14087_cov_8.090080"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1516)
                     /locus_tag="PKU75_08565"
     CDS             complement(35..1516)
                     /locus_tag="PKU75_08565"
                     /inference="COORDINATES: protein motif:HMM:NF040160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HPF80968.1"
                     /translation="MIAPRGAAAIRTAVGLVGAAGILAGAAALPDLDVRPVAGPSRAS
                     STPVTDAEFLCPGTELTGIDDVPDVSTSVRVVAGTAPAAVLGVDPPASTGAVTLAVAS
                     GPTVPDAAGGERTTQRGAVVTTTASEDGVVRAAGSGGLAPALLAAQESDPREDQVAGL
                     ASTACPAPAAESWLTGGSAEPGRQERLLLLNPGANPVTVDIAVAGRRGPIVTGAATGV
                     VVPARSRHSVLLDALAPDEAAPFFHVTTRGGFVGAVLADHWLDGVTPRGFADVPGLAA
                     PATEQVIPAVTGPGSVRLHAPGEQDAVVEVRLLTPDGPRALPDRAGVLRVPAGTTAEV
                     PLTKLPSGVSALQIRSDEPVVAAAQLTPVKARDFAWAPASAPIRGLAGAVYPALDSAA
                     DRTRTLALASTGGRARVGVTTVADGRAQTRELSIADDATLTLDLSLSDAVWVRRVAGN
                     GDVRAALLTTGGSGAKAVIDARPLTAARISVTTEPVEPVGPLG"
     gene            complement(1513..>2509)
                     /locus_tag="PKU75_08570"
     CDS             complement(1513..>2509)
                     /locus_tag="PKU75_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80969.1"
                     /translation="APRVVLGATSGGVPVHPWAGTGLLLMLAGLLGAALCAAPPAPTV
                     WSRPLAVCAGAGALALAVNLAVGGLGARLVPATDPRPAVAIDQASGPDATRTLRLLER
                     GAGFGYEFIGAEPGIPARDIATPAPSRALDDLVATLLDPAAGGTADPAGLLAQQAIGF
                     VIVAPGLPASLERQLDATSGLTRIGDYQQQAVWRVEPAAAGARAQPARLRLAAGDGWG
                     DPVAVRGSHGATSATISAPDGARLLVAEPAGWADHARVSADGVRLAGETTPAGVQSYA
                     VPAGAEELRVIVRPHHRVLGWAYLAALLFIGYLALPLGAAPARRTRDPHLQERAG"
     assembly_gap    2510..2609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2610..4630)
                     /locus_tag="PKU75_08575"
     CDS             complement(<2610..4630)
                     /locus_tag="PKU75_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80970.1"
                     /translation="MTAAPAAHLPYLAMAEPTPDPAVVPVTAVLLVARIDDRLVDALE
                     AVAAQTTRPDRLLILDATAAGDLAGHLGPDSALATRLPSHRIQRVALGTGLHAALAAA
                     CESTGEPGKQGALWALTGETVPDATALAELHTVLAADPAVGVAAPKLTDADRPGRLTR
                     FGIQVTRSGRLQLDPRPGDPDQQQFDDRTDALAAPAEGMLVRRAAYGALGGHLSSFGP
                     LGTDLDFGWRAHQAGYRVALAPRARVALAPHVLPLPSTADRRDARRVALARRPLLLMP
                     FLTVWTAVSTLAVALGLLALKRPAAAARQAGEAAAVLDPWRPLLARWRTRGQRVKQPA
                     STRGLFVTAAASRARASDRLHDVVVPQRSSGSTALRPADNVLDPGPALLAAPAIWGTL
                     LVAALTGVAARTLTSGVGAGLVRGWQGGELSRPFDADSTSLWHAWWDGWAGPGLGAAT
                     PASPGVAGLAVLSWITQHLPGAGDNPAGRTIALGMVLALPLAFLSAYLSAHVVTPARW
                     PRALAAAIWALSPAATGALAAGRLGALAILVLLPLMLAGFVRMARPTARPGSLALAAL
                     TTTALALVVPGAVVLGAGVALGLLLAGYAVARRRAVGYLLVLALAAIPVLLLVRDTPA
                     ALLGGWGLVAHGDSPPLWQIALGQPDVLPADGTALAALSDPRRWLLVPVL"
     gene            complement(4644..4901)
                     /locus_tag="PKU75_08580"
     CDS             complement(4644..4901)
                     /locus_tag="PKU75_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPF80971.1"
                     /translation="MRDVVYLDALDEEDLSWQERSLCAQTDPEAFFPEKGGSTREAKK
                     VCTGCEVRSECLAYALANDERFGIWGGLSERERRKLKRRAV"
     gene            5045..6034
                     /gene="cofD"
                     /locus_tag="PKU75_08585"
     CDS             5045..6034
                     /gene="cofD"
                     /locus_tag="PKU75_08585"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HPF80972.1"
                     /translation="MRITALAGGIGGARFLRGLRAALDADPVLAQSELTIVANTGDDI
                     TLFGLRVSPDVDTLLYTLGGGIDEAQGWGRADDSRRVQGELAAYGVQPQWFGLGDLDI
                     GTHIARSLWLGEGLTPSQITERLATRWGLPEQRIRLLPMSDSPVETHVIVERDGGPEA
                     IHFQQWWVGELAQTPAQRFVAVGLERAAPAPGVLEAIREADLILLPPSNPVVSIGIIL
                     GVPGVRDALRGAVAPVVGVSPIISGRPVRGHADTCLHVIGVESTAWGVAGLYADFLDG
                     WLVDPADAAGAPAGGPVAALTSRVYDGPLLMSDRPGAGRLARAALDLALELRG"
     gene            6031..7161
                     /locus_tag="PKU75_08590"
     CDS             6031..7161
                     /locus_tag="PKU75_08590"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPF80973.1"
                     /translation="MSARLEVLAVPGIGEIAPGTDLAVAIGDALLAADLAPGDGDILC
                     VTSKVISKARGLVAGTLDKRAAVAEQTVRVVAERMTATGVTRIVEGRHGVVMAGAGVD
                     ASNTGPEGQILLLPDDPDAAASDLLAGLTGYFRSRLGRSPDLGIVVSDTAGRAWRGGQ
                     TDFALGAAGVRVLEDYRGRLDADGRPMEVTAVAVADEIAAAADLVKGKVERVPVALVR
                     GLAHLVRTRHDTHHFTAAGDAPPSDPRAADVGARRLIRTGRGDFFAMGHIEGVRAALG
                     VPPGTDVASRAGIRSVTAESLAERLQRALRVALDNDPCGPAPVQAHIEHAETGDALVT
                     LHGTEDYAMGRARARLEVALWSESLTISGTSGDALRIAAAGV"
     gene            complement(7233..8198)
                     /locus_tag="PKU75_08595"
     CDS             complement(7233..8198)
                     /locus_tag="PKU75_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HPF80974.1"
                     /translation="MTETAAVDRPPADATFSGRERVYRPGQPLAIGAILGCFRHGAGD
                     PTFRRDDAGIWLGRHTPAGPGTLLVRWDAPAGEVRGRAWGAGADWLLDGLPALLGAED
                     DWGGFVAHHPQVAAAWRANPGWRVPRSRLVLDSLIPAVIEQKVTGKEAFAAYRTLVWR
                     FGDPAPGPREDLRLAPTAAQWRAIPSWQFLRAGVTPQRADTVMRCVQRAGRLEEAAGM
                     PLPAARARLQAVAGVGVWTAAEVAQRALGDPDSPSFGDYHVAKEIGWQLLGHDIDDDG
                     LAELLEPYAGHRYRVQALLALGGGPGRPRRGPRFTLPTHLPSRAW"
     gene            complement(8195..9031)
                     /locus_tag="PKU75_08600"
     CDS             complement(8195..9031)
                     /locus_tag="PKU75_08600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF80975.1"
                     /translation="MTTPHTPQHAAPPGGRALAVAKRGAEHVLWARVAGQRRLEALRG
                     TARPVPDSVPPTAVLQTRAEAEAAVAAAKALRLPLHHDRVKNWDALGAVGAVLALGGR
                     DGALPQRVIDAGSARYSPILPWLRLYGVPFLLGTNLEFGVPVRRDGVTFRYGDVTDTG
                     LPDGSVDAITCMSVIEHGVPIVPFLAESARILRPGGVLAISTDYDYDPPDTTGTSMYG
                     VPVHIFGPGEIRELVAQAGAAGLELVGELTEKALQHPERPCHWKRTGLDYTFVLLTFR
                     RR"
     gene            complement(9043..10041)
                     /locus_tag="PKU75_08605"
     CDS             complement(9043..10041)
                     /locus_tag="PKU75_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPF80976.1"
                     /translation="MTTGAAGVPSARPARRRAALATVRIVLAVLVLAGVVLALWRNWG
                     AVSSHLGDVSLRAWALALLAGFVSPFLGMLGWRALLTDLGSRLHIAPASGVFLVGQLG
                     KYLPGSVWSVFAQTEMAARLGVPRRRTGVVGLVTMALSLLTGVIIAVPSLPVLLRRGD
                     SSTAVLVGVAGMLFVIAMWPPLLNRGIRLGLRLLRRDPLEHDLSGRGIVTGGAWTLLA
                     WLATGAICWAFAADLVPDSADTGEVALISLSGFCLAAVVGMASVLLPAGVGVREGILL
                     LLLVTVMPGSAATAVVVLTRFVTLAMDILWAGIGWLWARAHHLLPDRHTVTVRPPD"
     gene            complement(10038..11861)
                     /locus_tag="PKU75_08610"
     CDS             complement(10038..11861)
                     /locus_tag="PKU75_08610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF80977.1"
                     /translation="MTPASSAALRVAIIGPAHPVKGGVTVHTTELAHQLADAGHEVEV
                     LSWSRMYPGFLYPGEVNVDLEHPETPLFEPTTRDLAWNRPDSWVRAGRGLGAYDLVVF
                     VHVIPQLIPAYLAMIAAIPGGEPPPRLVVLAHNVLPHEPRPGDERLVKSLFAKTDAVL
                     VHTPQQASLARSLGAETVRSAALPPHLPGGNPVTREPYAGPARLLALGLVRPYKGIAD
                     LVTALAQVPGPTLTVAGEAWGDAGAEVARAAALPGVEGRVTIRSGYVPADELAGLLAQ
                     HDVLALTYRTATASQNALLAHKHGLAVLATSVGSFPQDVRDEVDGLLVPARDQEALVA
                     VLRRLADPDVVAQLRAGVEDPDLTGPWANYVAALETLAALDLPEPEPSSVTWRDRVRG
                     AVVGLKPRVEVEANDLPDWITPTDILGLTSEADDARDVARRLGLPRRGDRESGWAALG
                     GLAAVLRVRDSGRTALIVDQSGPRSPFASWAKAIGFAPVEVATGEDLDVDPGSIDVLT
                     RLHPRDAEAGDLGDLFGQATWALRPGGLLITTIALGPATAPGVLGPADLRGVLAHADN
                     AGLRLVGDLDGDLTRRMRRAASVATDPDAAYGLARLTWRRR"
     gene            complement(11858..12841)
                     /locus_tag="PKU75_08615"
     CDS             complement(11858..12841)
                     /locus_tag="PKU75_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF80978.1"
                     /translation="MTELVTPAPGTPAPPTEVPAAAPHPEAPAPASAPTVTIVLPCYN
                     EGEHVLQEIERITAVMLASGLTYELFCIDDASTDNTLAVLKQAQLDYPNIRILPFRRN
                     GGSGTARRIGTQQAYGEIVVWTDADMTYENERIPELVQVLLDDPSYDQVVGARTTEEG
                     THKWARVPAKWLIRKVAERLTNQKIPDLNSGLRAFRRSVALPYLRLLPAGFSCVTTIT
                     IAFLSNQHDILYVPTSYAKRAGKSKFHFVQDAYRYILQVLRMVMYFDPLKVLMPLALW
                     LVGIGVVKGIVDMIRHPFYFPANTLLLLVSGLLIGAVALLADLVVRSRDGV"
     gene            complement(12847..>14087)
                     /locus_tag="PKU75_08620"
     CDS             complement(12847..>14087)
                     /locus_tag="PKU75_08620"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HPF80979.1"
                     /translation="QGAEGWASGHGPVSAIKGGSLFADHFSPITALFGLAWKVWPDPR
                     SLLVMQAVTLGIAVGMIAAFARERLGESFRAAAVLAALVSGLPLLSAAIAAPHETALG
                     APLYAGLGIALYRARFRAVAGWSLACLLVKEDSGLIIAGAALAWYLVHRRLRQSGLLL
                     ALGVAGFVASNAVIWWVRGSGSAYYGYVTESFGGLDPARALPAVMLLGAAGLVVRDPL
                     LLVAVPGIVWRSLSGNRNYWVPEFHYDVLTGIVGALVLIAAVQREGRMLPRRALVVGA
                     MAAVGLGTAIGERPWRADPLNRPEKEVVVSMLLTTIPAAGTLAADQSVGAYVPPDRHA
                     SMFSIDRQGTLALTPETTSCWVIMQPAQGDLSGTVAQKDQWLAGKAQVGVDPLVLAAT
                     GAKGCPAWDARNALPRKVPS"
BASE COUNT         1934 a         4940 c         5459 g         1654 t
ORIGIN      
        1 cctcaggcgt cgccggcctc aggcgtcgcc ggcctcagcc gagcgggccg accggctcga
       61 cgggttcggt ggtgacggag atgcgcgcgg ccgtcaacgg ccgggcgtcg atgacggcct
      121 tggcgccgct gccacccgtg gtgagcaggg cggcgcggac gtcgccgttg ccggcaacac
      181 gccggaccca gaccgcatcg ctcaggctca ggtcgagggt gagagtggcg tcgtcggcga
      241 tcgacagttc gcgcgtctgt gcccggccgt cggcgacggt ggtcacgccg acccgggcgc
      301 ggccgccggt ggatgccagc gccagagtgc gggtgcggtc ggcggcggag tcgagcgcgg
      361 gatagaccgc tccggccagc ccccggatcg gcgcgctcgc cggagcccag gcgaagtcgc
      421 gagccttgac cggcgtcagc tgggccgcgg cgacgaccgg ctcgtccgag cggatctgca
      481 gcgcgctcac cccggagggc agtttcgtca gcggcacctc ggccgtggtc ccggcgggca
      541 ctcgcaacac gcccgcccgg tccggcaggg cgcgcggtcc gtcgggggtg agcagccgga
      601 cctcgacgac ggcgtcctgc tcaccgggcg cgtgcaggcg gacgctgcct ggcccggtca
      661 cggccgggat gacctgttcg gtcgccgggg cggcaaggcc gggcacatcg gcgaaacctc
      721 gcggcgtgac cccgtccagc cagtggtcgg ccaggacggc gccgacgaag ccgccgcggg
      781 tcgtgacgtg gaagaagggc gcggcctcat ccggcgcgag cgcatcgagc agcacgctgt
      841 gccggctgcg ggcgggcacc acgaccccgg tcgcggcacc ggtgacgatg ggtccgcggc
      901 gcccggcgac ggcgatgtcg accgtgacgg ggttggcgcc ggggttgagc aggagcaggc
      961 gttcttgacg tccgggttcg gcgctgccgc cggtcagcca cgactcggcc gccggcgcgg
     1021 ggcaggcggt ggatgccagc ccggcgacct ggtcctcgcg ggggtcgctc tcctgggctg
     1081 cgagcagcgc gggggcgagc ccaccgctgc cggcagcgcg gacgacaccg tcttcggagg
     1141 cagtcgtcgt gacgacggcg ccccgctggg tcgtgcgctc gccgccggcg gcgtcgggca
     1201 ccgtcgggcc ggatgccacc gccagcgtca ccgcgcccgt cgaggccggc gggtcgacac
     1261 cgagcactgc ggcaggtgcc gtgcccgcga cgacgcgcac gctggtcgac acatcgggaa
     1321 cgtcatcgat gccggtgagt tcggttccgg gacaaaggaa ttcggcatcg gtcaccgggg
     1381 tcgacgacgc cctcgacggt ccggccaccg ggcggacgtc gagatccggc agggcggccg
     1441 ccccggcgag gatgcccgcg gcgccgacca accccaccgc ggtcctgatg gccgcggcac
     1501 ctcgaggggc gatcatccgg cacgctcctg caggtgcggg tcgcgggtgc gccgggcggg
     1561 ggcggcaccc aggggcaggg ccaggtagcc gatgaagagc agcgcggcga gataggccca
     1621 gccgagcacc cggtggtgcg gccggacgat gacgcgcagc tcctcggcgc cggcgggcac
     1681 ggcatacgac tgcacgccgg cgggggtggt ctcgcccgcg aggcggacac cgtcggcact
     1741 gacgcgtgcg tggtcggccc acccggccgg ctccgccacg aggagccggg cgccgtccgg
     1801 ggccgagatg gtcgcgctgg tggcgccgtg actgccgcgc accgcgaccg gatcacccca
     1861 cccgtcccca gcggccaggc gcaggcgggc gggctgggcc cgggcgccgg cggcggcggg
     1921 ctcgacgcgc catacggcct gctgctggta gtcaccgatc cgcgtcagcc cggaggtggc
     1981 gtcgagctgg cgttcgaggc tcgcgggcag gccgggggcg acgatgacga agccgatggc
     2041 ctgctgggcc aggagaccgg ccgggtcggc ggtgcctccg gcggcgggat cgaggagcgt
     2101 cgcgaccagg tcgtcgaggg cgcgggaggg cgcgggggtg gcgatatcgc gtgccggtat
     2161 gcccggctcg gcgccgatga actcgtagcc gaatccggca ccgcgctcca gcaggcgcag
     2221 ggtgcgggtc gcgtcgggac cgctggcctg gtcgatcgcc acggcgggac gcggatcggt
     2281 ggccgggacg agccgggcgc cgagcccgcc gacggcgagg ttgacggcca gggcgagcgc
     2341 cccggcgccg gcgcagacag cgagcgggcg gctccagacc gtgggcgccg gcggggccgc
     2401 gcagagggcg gcaccgagca gcccggcgag catgagcagc aggcccgtgc cggcccacgg
     2461 gtggaccggc acgcccccgg aggtggcacc gaggacgacg cgcggcgccn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcacggggac gagcagccac cggcgcgggt
     2641 ccgaaagggc ggcgagggcc gtgccgtcgg ccggcagcac atcgggctgg ccgagggcga
     2701 tctgccacag cggtgggctg tccccgtgcg cgacgagccc ccagcccccg aggagcgcgg
     2761 ccggcgtgtc gcgcacgagc aacaggaccg ggatcgcggc cagggccagg acgaggaggt
     2821 atccgacggc gcggcggcgc gccacggcat acccggcgag caacaggccg agcgcgaccc
     2881 cggcgccgag cacgacggcc cccgggacga cgagagccag agcggtcgtc gtcaaggcgg
     2941 ccagcgcgag gctccccggg cgggctgtcg gacgggccat gcggacgaat ccggcgagca
     3001 tgagcgggag cagcacgagg atcgcgaggg ccccgaggcg cccggcggcg agggcaccgg
     3061 tcgcggccgg gctcagggcc cagatggcgg cggcgagggc gcgtggccag cgcgcgggag
     3121 tgacgacgtg cgcgctgaga taggcactga ggaaggccag cggcagggcg agcaccatgc
     3181 cgagcgcgat cgtgcggccg gcagggttgt cgcccgcgcc gggcaggtgt tgggtgatcc
     3241 agctgagcac ggcgagcccg gcgacaccgg gtgaggccgg cgttgcggcg ccgaggccgg
     3301 gcccggccca gccgtcccac caggcgtgcc agaggctggt cgagtccgcg tcgaacggcc
     3361 ggctcagttc gccgccttgc cagccgcgga cgagaccggc cccgacgccc gaggtcaagg
     3421 tgcgcgccgc cacgcccgtg agggccgcga ccagcagggt gccccagatg gccggcgcgg
     3481 cgagcagtgc cggccccgga tcgagcacgt tgtcggccgg ccgcagggcg gtgctgcccg
     3541 aactgcgttg ggggacaacg acatcgtgca agcggtcgct ggctcgcgcc cggctggcgg
     3601 cggcggtgac gaacagccca cgggtcgatg ccggctgctt cacgcgctgg ccgcgggtgc
     3661 gccagcgcgc cagcagggga cgccacgggt cgaggacggc cgccgcctcg ccggcctggc
     3721 gggctgccgc cgccgggcgc ttgagcgcca gcaaacccag cgcaacagcg agcgtggaca
     3781 cggctgtcca taccgtgaga aacggcatga ggagcagcgg ccggcgggcc agtgcgaccc
     3841 ggcgagcgtc gcgccgatcg gcggtgctcg gcagtgggag gacatgcggg gcgagtgcga
     3901 cgcgcgcccg gggggcgagc gcgacgcgat aaccggcctg gtgcgcgcgc cacccgaagt
     3961 cgaggtcggt cccgagcggg ccgaagctgc tcaggtggcc gcccagggcg ccgtatgccg
     4021 cgcgccgcac gagcatgccc tcggccgggg cggcgagggc gtcggtgcga tcgtcgaact
     4081 gttgttggtc ggggtcgccg ggccgcgggt cgagctggag ccggccgctg cgggtgacct
     4141 ggatgccgaa ccgggtcagc cggcccggcc ggtcggcgtc ggtgagcttg ggggcggcga
     4201 ccccgacggc cgggtcggcg gcgaggacgg tatgcagctc ggcaagcgcc gtcgcgtcgg
     4261 gcacggtttc gccggtgagg gcccagagcg ccccctgctt ccccggctca cccgtgctct
     4321 cgcaggcagc ggcgagcgcg gcgtgcaggc cggtgccgag ggcgacccgt tggatgcggt
     4381 ggctgggcag ccgcgtggcg agggcgctgt cgggtccgag atggccggcg aggtcgccgg
     4441 cggccgtggc gtcgaggatg agcagccggt ccggccgggt ggtctgtgcc gcgacggcct
     4501 cgagggcgtc gacgagacgg tcgtcgatgc gcgcgacgag caggaccgct gtcaccggga
     4561 caacggccgg gtccggcgtg ggttcggcca tggcgaggta gggcaggtgg gccgcggggg
     4621 cggcggtcac cgggcgggac cggtcacacg gcgcggcgct tcagcttgcg ccgctcgcgt
     4681 tcggagaggc cgccccagat gccgaagcgc tcgtcgttgg ccagcgcgta ggcgaggcat
     4741 tcgctgcgca cctcgcagcc ggtgcagacc ttcttggcct cccgggtcga gccgcccttc
     4801 tcggggaaga acgcctcggg gtcggtctgg gcgcacaacg agcgctcctg ccaggagagg
     4861 tcttcctcgt ccagggcatc taggtaaacg acatcacgca cgggccactc ctcgccattt
     4921 ctgcctgaac tcgtgcgacg cgaggtcgga ttcccgccgc gccgcagcac cacaccagtg
     4981 gaattacacg cgtgtcatac cccggaagtc aagccattgc gaggtatggg tgaagattgg
     5041 ccacatgcga atcactgcct tggcgggtgg aatcggcggc gcgcgcttcc tgcgcggcct
     5101 gcgtgcggcc ctcgacgcgg accccgttct ggcgcaatcg gaattgacga tcgtcgccaa
     5161 caccggcgac gacatcaccc tgttcgggct gcgggtcagc cccgatgtcg acactctcct
     5221 ctataccctc gggggcggga tcgacgaggc gcaggggtgg ggccgagcag acgacagccg
     5281 gcgggtgcag ggcgaactgg cggcatacgg ggtgcagccg cagtggttcg ggctgggcga
     5341 cctcgacatc ggcacccaca tcgcccggtc gttgtggctc ggcgaggggt tgacgccgtc
     5401 gcagatcacc gagcggctgg cgacccggtg ggggctgccg gaacagcgga tccggctgct
     5461 gccgatgagc gactcccccg tcgagacgca tgtgatcgtc gagcgcgacg gcgggccgga
     5521 ggcgatccac ttccagcagt ggtgggtcgg ggagctggcc cagaccccgg ctcagcggtt
     5581 cgtcgccgtc gggctggagc gggcggcgcc ggcgccgggc gttctcgagg cgatccgcga
     5641 ggccgacctg atcctgctgc cgccgagcaa cccggtggtc tcgatcggga tcatcctcgg
     5701 cgtcccaggg gtgcgcgacg ccctgcgcgg cgccgtcgca ccggtcgtcg gtgtctcgcc
     5761 gatcatcagc ggccgcccgg tgcgcgggca cgccgacacc tgcctgcacg tcatcggcgt
     5821 cgagtccacg gcctggggcg tcgcgggcct gtatgccgat ttcctcgacg gctggctcgt
     5881 ggaccccgcc gatgccgcgg gggctcccgc cggcgggccg gtggccgcgc tgacgagccg
     5941 ggtgtatgac gggcccctcc tcatgagcga ccgtcccggc gccgggcggc tggcccgggc
     6001 ggcgctcgat ctggccctgg agttgcgcgg gtgagtgccc ggctggaggt gctggcggtc
     6061 cccgggatcg gggagatcgc gcccggcacc gacctggccg tggccatcgg cgacgcgctg
     6121 ctcgcggccg acctggcgcc cggcgacggc gacatcctct gcgtgacgtc gaaggtcatc
     6181 tccaaggcgc ggggcctggt ggccggcacc ctcgacaagc gggccgccgt cgccgagcag
     6241 acggtgcgcg tcgtcgcgga gcggatgacg gcgaccggtg tcacccggat cgtcgaaggc
     6301 cgccacggcg tggtgatggc cggtgccggg gtggacgcct ccaacaccgg gccggagggt
     6361 cagatcctgc tgctccccga cgacccggat gccgccgcga gcgacctgct cgccggtctc
     6421 acgggctact tcaggtcaag gctggggcgc agtcccgacc tgggcattgt cgtctcggac
     6481 acggccgggc gggcctggcg cggcgggcag accgacttcg cgctcggggc cgccggggtg
     6541 cgggtgctcg aggactatcg cggccgcctc gacgccgacg gccggccgat ggaggtgacg
     6601 gccgtcgccg tcgccgacga gatcgccgcg gccgccgacc tcgtcaaggg gaaggtcgag
     6661 cgggtgccgg tggcgctggt gcgcgggctg gcgcacctgg tgcgcacccg gcacgacacc
     6721 caccacttca ctgctgcggg cgacgcgcca cccagcgatc cgcgggctgc cgacgtgggg
     6781 gcgcggcgcc tgatccgcac cgggcgcggg gacttcttcg cgatgggcca catcgaggga
     6841 gtgcgggccg cgctcggtgt cccaccaggc actgacgtgg ccagccgggc ggggatccgc
     6901 tcggtgaccg cggagtcgtt ggccgaacgg ctgcagcggg ccttgcgcgt cgcgctcgac
     6961 aacgacccct gcggcccggc acccgtgcag gcgcacatcg agcacgccga gaccggcgat
     7021 gcgctcgtca cgctccacgg caccgaggat tacgctatgg gtcgcgcgcg cgcccgcctc
     7081 gaggtcgccc tctggtcgga gtccctgacc atcagcggga cctcgggcga tgcgctgcgc
     7141 atcgcagcgg ccggagttta gctgcgagcc gtcacacctg gggtagcagg gtcgtcacgg
     7201 ccccggtcgt ggtgttccgc acgaccagcc cgtcaccacg cgcgagaggg caggtgggtg
     7261 ggcaacgtga agcgaggccc gcggcggggg cggccgggac cgccgccgag agcgagcagg
     7321 gcctggacgc ggtagcggtg accggcatac ggctcgagca actccgcgag cccgtcgtcg
     7381 tcgatgtcgt gaccgagcag ctgccagccg atctccttgg cgacgtgata gtcgccgaag
     7441 ctcgggctgt ccggatcgcc gagggcgcgc tgggccacct cggccgccgt ccacaccccg
     7501 acccccgcga cggcctgcag gcgcgcgcgg gcggcgggca gcggcatacc ggccgcctcc
     7561 tccagccggc cggcccgctg cacacagcgc atcaccgtgt cggcgcgctg cggcgtcacg
     7621 cccgcgcgga ggaactgcca cgacgggatc gcccgccact gcgccgcggt gggcgccagc
     7681 cgcaggtcct cccggggtcc gggcgcgggg tcgccgaagc gccacaccag cgtccggtat
     7741 gccgcgaacg cctccttgcc cgtcaccttc tgttcgatga cggccgggat cagcgagtcg
     7801 agaaccagcc gcgagcgcgg cacccgccag cccgggttgg cccgccacgc agccgcgacc
     7861 tgcgggtggt gggcgacgaa cccgccccag tcgtcctcgg cgccgagcag cgcgggcagg
     7921 ccgtcgagca gccagtccgc ccccgcgccc caggccctcc cacgcacttc gccggccggc
     7981 gcatcccagc gcaccagcag ggtcccgggc ccggcggggg tatgccgccc cagccagatc
     8041 ccggcgtcgt cgcgccggaa cgtcgggtcg ccggcgccgt gccggaagca gccgaggatg
     8101 gcaccgatcg cgagcggctg cccgggccgg tagacgcgtt cgcgtccgga gaaagtggcg
     8161 tccgccgggg gccggtccac cgcggccgtc tcggtcaccg gcgccggaag gtcagcagga
     8221 cgaaggtgta gtccaggccg gtgcgcttcc agtggcaggg ccgctcgggg tgttgcaacg
     8281 ccttctcggt caactccccc accagctcga gaccggccgc gcccgcctgg gcgaccagct
     8341 cccggatctc gccgggcccg aagatgtgca ccggcacgcc atacatcgac gtgccggtcg
     8401 tgtccggcgg gtcgtagtcg tagtcggtcg agatcgccag caccccgccg ggccgcagga
     8461 tgcgcgcgga ctcggccagg aacggcacga tcggcacacc gtgctcgatc accgacatgc
     8521 acgtgatcgc atcgacggac ccgtcgggca ggccggtgtc ggtgacatcg ccgtagcgga
     8581 acgtcacgcc gtcgcggcgg accggcaccc cgaactcgag gttggtgccg agcaggaagg
     8641 ggacgccgta caaccgcagc cacggcagga tcgggctgta gcgggcgctg cccgcgtcga
     8701 tcacgcgctg cggcaacgcc ccgtcacgtc cacccagcgc gaggacggcg ccgacggccc
     8761 cgagggcatc ccagttcttg acccggtcgt ggtgcagcgg cagtcgcagc gccttggccg
     8821 cggcgacggc cgcctccgcc tcggccctcg tctgaaggac ggcggtcggg ggcaccgagt
     8881 ccggcaccgg ccgggcggtg ccgcgcaggg cctccagccg ccgctgcccg gcgacccgcg
     8941 cccagagcac atgctcggcc ccgcgcttgg cgacggccag agcgcgtccg cccgggggtg
     9001 cggcgtgttg cggggtatgc ggtgtggtca tggatggtca acctagtcgg gtgggcggac
     9061 agtgacggta tgccggtcgg gcagcaggtg gtgcgcgcgg gcccacagcc agccgatgcc
     9121 ggcccagagg atgtccatcg ccagggtcac gaagcgggtg agcacgacga ccgcggtcgc
     9181 ggccgacccg ggcatgacgg tgacgagcag gagcagcagg atgccctcgc gcaccccgac
     9241 cccggccggc agcagcaccg acgccatgcc gacgacggcc gcgaggcaga acccgctcaa
     9301 cgagatcagc gccacctcgc cggtgtcggc gctgtcgggg acgaggtcgg cggcgaaggc
     9361 ccagcagatc gcgccggtgg ccagccacgc gagcagggtc cacgccccgc cggtgacgat
     9421 gccgcggccg ctgaggtcgt gttcgagcgg gtcgcggcgc agcagccgca aacccaggcg
     9481 gatgccgcgg ttgagcagcg gcggccacat cgcgatgacg aacagcatcc ccgcgacccc
     9541 gacgaggacg gccgtgctgg aatcgccccg gcgcagcagg accggcagag acggcaccgc
     9601 gatgatgacg ccggtcagca gcgacagggc catggtgacc aggccgacga cgccggtgcg
     9661 acgccgcggc acgccgaggc gggcggccat ctcggtctgg gcgaagacgc tccacaccga
     9721 gccgggcagg tacttgccga gttgcccgac gaggaagacc ccgctggccg gggcaatgtg
     9781 caggcgggag ccgaggtcgg tcagcagcgc ccgccagccg agcatgccga ggaagggcga
     9841 gacgaatccg gcgagcagcg ccagcgccca cgcccgcagc gagacgtcgc cgaggtggga
     9901 ggagacggca ccccagttgc gccagagcgc gagcaccacc cccgccagca cgaggacggc
     9961 caggacgatc cggacggtcg ccagggcggc ccggcgacgg gccgggcgcg cggaaggtac
    10021 gcccgctgcc ccggtcgtca ccggcgtcgc caggtcagcc gggccaggcc gtatgccgcg
    10081 tccgggtccg tcgcgacgct cgccgcccgc cgcatccggc gcgtcaggtc gccgtcgaga
    10141 tcgccgacga gccgcaggcc cgcgttgtcg gcgtgcgcca gcacgccgcg caggtccgca
    10201 ggcccgagca ccccgggcgc ggtcgccggt ccgagcgcga tcgtcgtgat gagcagccct
    10261 ccgggccgca gcgcccacgt cgcctgaccg aacaggtcac cgaggtcgcc ggcctccgcg
    10321 tcgcgcggat gcagccgggt gagcacgtcg atcgacccgg ggtcgacgtc gaggtcctcc
    10381 cccgtcgcga cctcgaccgg ggcgaacccg atcgccttcg cccaggacgc gaagggcgaa
    10441 cgcgggccgg actggtcgac gatcaacgcg gtgcggccgg agtcgcgcac ccgcaacacg
    10501 gcggctagtc cgccgagggc ggcccagccg ctctcccggt cgccgcgtcg cggcaggccg
    10561 agccggcggg cgacgtcgcg cgcatcgtcc gcctccgagg tcaggccgag gatgtcggtc
    10621 ggggtgatcc agtcgggcag atcattcgcc tcgacctcca ctcgaggctt aagtccgacg
    10681 accgctcctc tgacccggtc tcgccaggtc acggaggacg gttcgggttc gggcaggtcg
    10741 agggccgcga gggtctccag cgccgccaca tagttggccc acgggccggt cagatcggga
    10801 tcctcgacgc cggctcgcag ctgcgcgacc acgtcgggat cggcgagccg gcgcagcaca
    10861 gcgacaaggg cctcctggtc gcgcgccggg acgagcagcc cgtcgacctc gtcccgcacg
    10921 tcctggggga agctgcccac gctggtggcg aggaccgcca gtccgtgctt gtgggcgagc
    10981 agcgcgttct gcgaggcggt cgcggtgcga taggtcaggg ccagcacgtc gtgctgggcg
    11041 agcaggccgg ccagctcgtc ggcgggcaca tagccggagc gaatcgtcac ccggccctcc
    11101 accccgggca gggcggccgc ccgcgccacc tcggcaccgg cgtcacccca cgcctccccc
    11161 gccaccgtca gcgtcggccc gggcacctgc gcgagcgcgg tgacgaggtc ggcgatccct
    11221 ttgtaggggc ggaccaggcc gagcgcgaga agccgggccg ggccggcata cggctcgcgg
    11281 gtcaccggat tcccacccgg caggtgcggc ggcagggcgg cgctgcgcac cgtctcggcg
    11341 ccgagcgagc gggccaggct cgcctgctgc ggcgtgtgca cgagcaccgc gtcggtcttc
    11401 gcgaagaggc tcttgacgag ccgctcgtcg cccggtcgcg gctcgtgcgg cagcacgttg
    11461 tgggcgagca cgaccagccg cggcggcggc tcgcccccgg gaatggcggc gatcatcgcc
    11521 aggtaggccg ggatcagctg cgggatgacg tgcacgaaga cgacaaggtc gtaggcgccc
    11581 aggccgcgtc cggcgcgcac ccacgagtcg gggcgattcc acgccaggtc gcgggtcgtg
    11641 ggttcgaaga gcggggtctc ggggtgttcg aggtcgacgt tgacctcgcc cggatagagg
    11701 aacccggggt acatccgcga ccacgagagc acctccacct cgtgcccggc gtcggcgagc
    11761 tggtgcgcga gttcggtcgt atgcaccgtc acgcccccct tgaccgggtg cgccgggccg
    11821 atgatcgcga cgcgcagggc ggcgctggag gcgggcgtca taccccgtct cgggagcgca
    11881 cgacgaggtc ggccagcagc gccacggcgc cgatgagcag cccgctgacc agcagcaaca
    11941 gggtgttcgc cgggaagtag aaggggtggc ggatcatgtc gacgatgccc ttgacgacgc
    12001 cgatgccgac cagccacagg gccagcggca tgagcacctt gagcgggtcg aagtacatca
    12061 ccatccgcag cacctgcagg atgtacctgt aggcgtcctg gacgaagtgg aacttgctct
    12121 tgccggcccg tttcgcataa ctcgtcggca catagaggat gtcgtgctgg ttggacaaga
    12181 aggcgatcgt gatggtcgtg acgcacgaga acccggccgg cagcagccgc aggtagggca
    12241 gggcgacgct gcgccggaag gcgcgcaggc cggagttgag gtcggggatc ttctggttgg
    12301 tcaggcgctc ggcgaccttg cggatcagcc acttcgcggg cactcgcgcc cacttgtgcg
    12361 tgccctcctc ggtggtgcgg gccccgacga cctggtcgta tgacgggtcg tcgagcagca
    12421 cctggacgag ctcggggatg cgctcgttct cgtaggtcat gtcggcgtcg gtccacacga
    12481 cgatctcgcc gtatgcctgc tgggtgccga tgcggcgcgc ggtgccggag ccgccgttgc
    12541 ggcggaaggg caggatccgg atgttcgggt agtccagctg cgcctgcttc agcaccgcga
    12601 gcgtgttgtc ggtggaggcg tcgtcgatgc agaacagctc gtaggtcagg ccgctggcca
    12661 gcatgaccgc ggtgatgcgc tcgatctcct gcagcacgtg ttcgccctcg ttgtagcagg
    12721 gcagcacgat cgtgacggtc ggggccgacg ccggcgcggg cgcctcgggg tggggtgcgg
    12781 ctgcgggcac ttcggtcggg ggcgccgggg tgcccggggc gggggtgacg agctcggtca
    12841 ttgtggctac gagggtacct tccgcggcag cgcgttccgg gcgtcccacg cgggacaccc
    12901 cttcgcgccg gtcgccgcga ggaccagggg gtcgacaccc acctgcgcct tgccggcgag
    12961 ccactgatcc ttctgcgcga cggtgccgga cagatcgccc tgggccggct gcatgatcac
    13021 ccagcaactc gtcgtctccg gcgtgagggc caaggtgccc tgccggtcga tcgagaacat
    13081 cgacgcgtgc cggtcgggcg ggacgtaggc gccgacggac tggtcggccg cgagcgtccc
    13141 tgcggcgggg atcgtggtca ggagcatcga cacgaccacc tccttctccg gccggttgag
    13201 ggggtcggcg cgccacggcc tctccccgat ggcggtgccg agcccgacgg cggccatggc
    13261 accgaccacc agggcgcggc gcggcagcat ccgcccctcc cgttgcacgg cggcgatcag
    13321 gacaagggct cccacgatgc cggtcaggac gtcgtagtgg aactcgggca cccagtagtt
    13381 gcggttgccc gagaggctgc gccacacgat cccggggacc gcgacgagca ggagtgggtc
    13441 gcgcacgacg aggccggccg cgcccaggag catcaccgcc ggcagggccc gggcggggtc
    13501 caggccgccg aaggactcgg tgacgtagcc gtaataggcg ctccccgagc cccgcaccca
    13561 ccagatgacg gcgttgctgg cgacgaaacc ggcgaccccg agcgccagga ggagcccgct
    13621 ctggcgcagg cgacggtgca cgagatacca ggccagcgcc gcgccggcga tgatgagccc
    13681 ggagtcctct ttgacgagca agcaggcgag cgaccagccc gcgacggcac ggaaccgggc
    13741 gcgatagagg gcgattccta gaccggcata cagcggcgcg ccgagggcgg tctcgtgcgg
    13801 ggcggcgatg gccgccgaga gcagcgggag cccggagacg agcgcggcga gcacggccgc
    13861 ggcacggaac gactccccca gccgctcccg ggcgaaggcg gcaatcatgc cgaccgcgat
    13921 gcccaaggtc acggcctgca tcacgagcaa cgagcgcgga tcgggccaga ccttccacgc
    13981 gagcccgaag agcgcggtga tggggctgaa gtggtcggcg aacaggctgc cccccttgat
    14041 cgccgacacc ggcccgtgac cgctcgccca cccctccgct ccttggg
//
LOCUS       DAOPJG010000077        13924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4606_length_13924_cov_8.075781, whole genome shotgun sequence.
ACCESSION   DAOPJG010000077 DAOPJG010000000
VERSION     DAOPJG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4606_length_13924_cov_8.075781"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="manB"
                     /locus_tag="PKU75_08625"
     CDS             <1..695
                     /gene="manB"
                     /locus_tag="PKU75_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482361.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPF80980.1"
                     /translation="DADRCFVVDERGEPVSASAITALVAMREIAKEAARGIPADQVRI
                     VHNVISSRAVTEFIVRAGAQPVRTRVGHSFIKGEMAKHDAVFGGEHSAHYYFREFWFA
                     DTGMLAAMHVLAALGEQEGPLSALVAEYAPYASSGEINSTVADAAGITERVAAWAGER
                     GLRMDRLDGLTVWHPTDESEPMWWFNLRSSNTEPLLRLNVEAADPATMERVRDTLLGM
                     IRGAAPAADRGE"
     gene            700..897
                     /locus_tag="PKU75_08630"
     CDS             700..897
                     /locus_tag="PKU75_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80981.1"
                     /translation="MTNPSDTIEPWVRELLRCPKCLTTLRDDSRDGAPELVCESAECG
                     LAYPVEDGIPVLLIDKARPRG"
     gene            901..2142
                     /locus_tag="PKU75_08635"
     CDS             901..2142
                     /locus_tag="PKU75_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPF80982.1"
                     /translation="MPFEIERLDDTEALAETDRSGMLRALATAGAQVRRAVTATREAG
                     LLERDPDDRPRAVLVAAMGADNLVGDLVCALAARHSPVGVWTAHDLPLPGWVGSLDLV
                     VAVSLSGRAPGPLRLAHEAGRRGATVLSIGAPDSPLAEATARARGVHIAVEREAAGVR
                     LASRSAAWSMLTPVVLALAAHGLVDVSPDDLAQVADRLDAVAEAARPGSESFVSPPKI
                     LAGEIAGRLPMILGDGPLTAVAARRAGTMLARTARIPVMVGELPDAAAELLACLDGPY
                     AAGAPTGPDGGRDIFADPFLDGPPAPEVGLLLVRDRDPRAGEEGSAVRASGAAAYPEP
                     NAPIGPQDAARINLTQAIVDLALARGTRVHELTPGPGPDIVRLAELIAQIDFAATYLA
                     LGHGLDPAAAPAVADLRDLTS"
     gene            2312..3763
                     /gene="ahcY"
                     /locus_tag="PKU75_08640"
     CDS             2312..3763
                     /gene="ahcY"
                     /locus_tag="PKU75_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPF80983.1"
                     /translation="MSFDFKVKDLALAESGRHQIRLAEHEMPGLMSLRTEFGGAQPLK
                     GAKIAGSLHMTVQTAVLIETLVALGAEVRWASCNIFSTQDEAAAAVVVGPDGTTANPQ
                     GVPVFAWKGESLPEYWECTDAILTWPGGDGPNMILDDGGDATMLVHKGKEWERAGQVP
                     PVTEEDSEEFGVFKELVRATMAADPQKWTRIADGIKGVTEETTTGVHRLYQLAEAGEL
                     LFPAINVNDSVTKSKFDNKYGCRHSLVDGLNRATDVLIGGKVAVVAGYGDVGKGCAES
                     LRGQGARVIVTEIDPICALQAAMDGYQVARLDEVVGAGDIFVTATGCFDVITAEHMQA
                     MKNKAIVANIGHFDNEIDMAGLAKVEGVTKTEIKPQVHEWTFADGRSIIVLSEGRLMN
                     LGNATGHPSFVMSNSFSNQTIAQMELFAHPESYCDEDGKPTVTTLPKHLDEKVARLHL
                     EALGVELTELTKEQAEYLGVDVAGPFKPEHYRY"
     gene            4181..4861
                     /gene="mtrA"
                     /locus_tag="PKU75_08645"
     CDS             4181..4861
                     /gene="mtrA"
                     /locus_tag="PKU75_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HPF80984.1"
                     /translation="MKGRVLIVDDDLALAEMLGIVLRGEGLDVVHVADGSAAVGAFRD
                     HKPDLILLDVMLPGLDGIQVCRRIRAESGVPIVMLTARTDTVDIVVGLESGADDYVVK
                     PFKPAELIARVRARLRRGEEAEPERLEIGDLAIDVAGHSVTRDGKQIPLTPLEFDLLV
                     ALARKPWQVYTREVLLEQVWGYRHAGDTRLVNVHVQRLRSKIEHDPEDPKIVVTVRGV
                     GYKAGPGG"
     gene            4878..6521
                     /gene="mtrB"
                     /locus_tag="PKU75_08650"
     CDS             4878..6521
                     /gene="mtrB"
                     /locus_tag="PKU75_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HPF80985.1"
                     /translation="MRRLLRGLLAPWRRSLQLRVVASTVLLGILAISLLGAYLFQKVA
                     DGLTEDRIGYAEREAAVLAARAQTAFDQTDATGDISEFNNFASGAIVALAPPANDTSR
                     YVVFLRSQDNTNAVRLDTVLTGGLRTSPVPVALREAISSDVGRQQRMVIELDRSELVG
                     EPSANWARARIPAVIVGQQIQVPTAGPYDLYFIYPLDREQATMDLIRRLFLIGGLLLV
                     TLVGGIAWLVTRQVVNPVRRAAGVAERLAAGHLNERMSARGADDLALLGTSFNGMADH
                     LQSQIRQLEDLSRVQQVFVSDVSHELRTPLTTIRMAADLIHEGRGEFSPALGRSAELL
                     NTELDRFEALLADLLEISRFDAGAAALELDRVDLGALAERVVQAAAPLADKRGSTVTV
                     DVAPGGVFADADARRVERIVRNLLVNAIEHGEGLPVTIRVAANSTAAAIAVHDLGVGL
                     RPGEASLVFNRFWRADPARARTTGGTGLGLAISLEDARLHDGWLQAWGEAGRGSVFRL
                     TLPRVAGEPIGESPLPLAPEPTDVDATTRLRPVRLTETR"
     gene            6518..8278
                     /locus_tag="PKU75_08655"
     CDS             6518..8278
                     /locus_tag="PKU75_08655"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HPF80986.1"
                     /translation="MTARRARALLGALLALLLSGCVGLHADPRIQPGLGVGPADRDEV
                     GFIPPGPQDGSSPEAVIRGFLRAAGVSGQGVAVARTYLTQSLAASWNPEDRAVVTEEG
                     IEAFLDQVATDTYSLSGTLTGTVDAQGRYEPAASATRTTARINVTQVAGQWRISGLPD
                     GFGRWLSKAAFRQLMTPYDFYYVGPDDALVPDVRYVATDKLATRLTRGELGPVPAYLG
                     GAVRTDLGPGVRLSVDAVPVEGGVAAIDLAAAHVSADPAARRRMWAQLVATVTQAPNV
                     SSIAITVEGAGLDLGGLNPPVSGIDALGFQETAAPVGVLPLLRLGRGLVPTDPARIGQ
                     PDQGGIAEQPSAYPRIERTFTELALAFTGQEVAAVAEGELARFRASAGASVPNFGSNL
                     TGPAYDRKGFLWVGGRMSGGRHDGAVWVVDSGIWPPANAPARRITASWLAGRRPVSIA
                     IALDGARVAVISTDDKGRDARLDIAGIVRDDLGAPAGLAESPLRLAPMLSTLRDVVWL
                     SESDVAVLGGETKQALRPYVVTLGQQVTPLPEVKGAERIVTLGGERRLFVRTADELYM
                     RAGGRWAEVGVGDDLVVPGR"
     gene            8373..9086
                     /locus_tag="PKU75_08660"
     CDS             8373..9086
                     /locus_tag="PKU75_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPF80987.1"
                     /translation="MRRRIDIGGLIDLALPRACAGCGLPGPSVCPDCVEDIAATTFAG
                     GPKPILPWPAPRGLGPVCTAGAYSGALASVIAGHKDAGRDDVRSLLALLLARAIRAAR
                     VPPDAVVVGLPSSPAATRRRGDAPVARLVAHAAAAGQLPAIDALRVSGRVRDAVGLSS
                     AQRARNISGVMRASRDVAGQVVVLADDIVTTGATMREAVRAIEAAGGVVVARAAIAAT
                     PRRFREEQGESLHNGTEVS"
     gene            9273..9893
                     /gene="raiA"
                     /locus_tag="PKU75_08665"
     CDS             9273..9893
                     /gene="raiA"
                     /locus_tag="PKU75_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPF80988.1"
                     /translation="MDVVISGRHQTVSDRFRDHVTERVGKVEQLAPRALRVEVLVSHE
                     PARSKAKATERVEITCHARGPVVRAEATHDDKYAAFEQAMDKLLERLRRANDRRRVAR
                     TRGKKPAEVPLEAVLTEPAPALEVDDERDLFGAIGDSPIEVREKVHQSAPMTLAEAMA
                     EMELVGHDFFLFQDSETGKPSVVYRRHGWNYGVIHLEVAAPAEAAS"
     gene            9909..>11047
                     /locus_tag="PKU75_08670"
     CDS             9909..>11047
                     /locus_tag="PKU75_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPF80989.1"
                     /translation="MRLSQAQARRIALAAQGFGRPRGVREPTMRDIQRVIDTVGCIQI
                     DSVNVVARSQYLPFFSRLGPYDVSLLDRARDRAPRRLVEYWAHEASLIPPTTWPLLDF
                     RMQRALSDSWGGMQSVARTRPDLVAAVLTEIEARGPLTSRQTEIALEHDVPRSKEGWG
                     WNWSLVKNALEHLFWGGQIGSSGRTTQFERKYDALHRVLPPAVHPHAIEASDRPPVET
                     AYRQLVEIAARAHGVATVQCLRDYFRLPVKATQPAIDRLVADDVLIPVEVEGWRRAAY
                     LHRDARRPRRVEARALLSPFDSLVWTRDRVSALFGFDYRLEIYVPAPKRVHGYYVLPF
                     LLGDELVGRADLKADRAAGVLRVQRLTWEPGRGGSSDRSELEAEL"
     assembly_gap    11048..11147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11148..11884)
                     /locus_tag="PKU75_08675"
     CDS             complement(<11148..11884)
                     /locus_tag="PKU75_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80990.1"
                     /translation="MNTPARLLTSALAVTAAVGLAATGSHATSPGRPGALAYVGLDHS
                     IYSDRLVAGQVMTKRLTPGVGDHPRWPQDGTKLAYATGARNIVVMKPDGTAKKTVSVG
                     RSWQPNWMPVTGKPSQVGFVKVINGKGDIYAVPAAGGVAKRLTTDGATTCGNSQPAWS
                     ADGTYFAYVQRTKQAAGCGSHRLVVITRATGVKRVVQTVIWPDDGVTPLGVGDDRIDF
                     TADSAQIWFSLSGNTDCVETSGVYAVAT"
     gene            complement(11978..13099)
                     /locus_tag="PKU75_08680"
     CDS             complement(11978..13099)
                     /locus_tag="PKU75_08680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF80991.1"
                     /translation="MAFPDVVPTLTDGVVLLRAHRPSDAEGIVAQSNDPASMRWTTVP
                     RPYGMQQAQEFRTLIEDGWNAGTGNLHWAIAGAADPDEYLGTIDLRHGPGGTAEVGFG
                     LHPAGRGRGLMSRAVRLLAGYYFGDLGGHRLYWYAARGNFASWRVAWACGFQHHGLLP
                     QKLAPAPGDPEAVALDAWAASVGADDDLTKPKAPWFEPPTLESDGIRLRGWRDDDLAV
                     AEPHDHPSHHVPERAIPTAETFEAWLMRRRELMSQGTSVNWCIADPATDEPVGEMLVF
                     VHHGSMTDGDTAELGYFLKPSARGRGFASRATRLATAYALSPDGLGLRRLVAETAADN
                     AVSNRILESAGFTRWGHEAQATAPGGSVGPADHWERVRQ"
     gene            13268..>13924
                     /locus_tag="PKU75_08685"
     CDS             13268..>13924
                     /locus_tag="PKU75_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPF80992.1"
                     /translation="MPKFVERLLRAGEGRTVKRLESIAAQVNAIEEDFEKLTDAELRA
                     ETDVFKQRLADGATLDDILPEAFAAVREASKRTLGKRHFDVQIMGGAALHQGNVAEMK
                     TGEGKTLVATLPSYLNALTGKGVHVITVNDYLAAYQSELMGRIHRMLGIETGCILSDM
                     TPEQRRAEYAKDITYGTNNEFGFDYLRDNMAWSTDELVQRGHNFAIVDEVDSILIDEA
                     R"
BASE COUNT         2001 a         5123 c         4743 g         1957 t
ORIGIN      
        1 gcgacgccga ccgctgcttc gtcgtcgacg agcgcggcga accggtctcc gctagtgcga
       61 tcaccgccct cgtcgcgatg cgcgagatcg ccaaggaggc ggcccgcggc ataccggcgg
      121 atcaggtgcg gatcgtccac aacgtcatct ccagccgggc ggtgaccgag ttcatcgtcc
      181 gcgccggcgc ccagccggtg cgcacccgtg tcggccactc cttcatcaag ggcgagatgg
      241 ccaagcacga cgccgtcttc ggcggcgagc acagcgcgca ctactacttc cgcgagttct
      301 ggttcgccga caccggcatg ctcgccgcga tgcacgtgct cgccgcgctc ggcgaacagg
      361 agggcccgct gtcggcgctg gtggccgagt acgcgccgta tgccagttcg ggcgagatca
      421 actcgacggt cgccgatgcg gcgggcatca ccgaacgcgt tgccgcgtgg gcgggggagc
      481 gcgggctgcg gatggaccgg ctcgacgggc tgaccgtgtg gcacccgacc gacgagagcg
      541 agccgatgtg gtggttcaac ctgcgctcct ccaacaccga acccctgctg cggctcaacg
      601 tcgaggccgc cgatcccgcc accatggagc gggtgcgcga cacgctgctc ggcatgatcc
      661 gtggcgccgc acccgccgcc gaccgaggag aatgacgcca tgacgaatcc ttccgacacc
      721 atcgagccct gggtgcgcga gttgctgcgg tgccccaaat gcctgaccac gctgcgcgat
      781 gactcccgcg acggcgcccc cgagctggtg tgcgagtccg cggagtgcgg cctggcctat
      841 ccggtggagg acggcatacc ggtgctgctc atcgacaagg cccgcccccg gggctgaccg
      901 gtgcccttcg agatcgagcg cctcgacgac accgaggcgc tggccgagac cgaccgctcc
      961 gggatgctgc gcgccctggc gacggcgggt gcgcaggttc gccgggccgt cacggccacg
     1021 cgcgaggccg gcctgctcga gcgcgacccc gatgaccgcc cgcgggccgt gctggtcgcg
     1081 gcgatgggcg ccgacaacct cgtcggcgac ctcgtctgcg ccctcgccgc ccggcattcg
     1141 ccggtggggg tatggacagc gcacgacctc ccgctgcccg gttgggtcgg atcgctggat
     1201 ctcgttgtcg ccgtgtcact ttcgggtcgc gccccgggcc cgctgcggct cgcgcacgag
     1261 gccggccgcc gcggcgccac cgtgctcagc atcggcgcgc cggacagccc gctcgccgag
     1321 gcgaccgccc gggcgcgcgg ggtccacatc gccgtcgaac gcgaggccgc cggggtgcgc
     1381 ctcgcgtccc gctcggcggc gtggtcgatg ctcaccccgg tcgtcctggc gctcgcggca
     1441 cacggcctcg tcgacgtctc gcccgatgac ctggcacagg tggccgaccg cctcgacgca
     1501 gtcgccgaag ccgcccggcc ggggtcggag tccttcgtca gcccgcccaa gatcctcgcc
     1561 ggcgagatcg ccgggcggct gccgatgatc ctcggcgacg gcccgctcac cgcggtcgcg
     1621 gcgcggcggg ccggcacgat gctcgcccgc accgcccgca tcccggtcat ggtcggcgag
     1681 ttgcccgacg ccgccgccga actgctcgcg tgcctcgacg ggccgtatgc cgcgggcgcc
     1741 cccaccggtc ccgacggcgg gcgcgacatc ttcgccgatc ccttcctcga cggcccgccc
     1801 gcccccgagg tcggcctgtt gctcgtgcgc gaccgcgacc cgcgggcggg cgaggagggg
     1861 tccgcggtcc gggcgagcgg ggccgcggca tacccggaac ccaatgcacc aatcggaccg
     1921 caggacgcgg ctcgcatcaa cctgacccag gcgatcgtcg acctggcgct ggcccgcggc
     1981 acccgcgtcc acgaactcac gcccggcccc ggccccgaca tcgtccggct cgccgaactg
     2041 atcgcccaga tcgacttcgc ggccacctat ctcgccctcg ggcacggcct cgatcccgcc
     2101 gccgcgccgg ccgtcgccga cctgcgtgac ctgaccagct agccggccgg cgcccgctag
     2161 actgaaggct ccggcgtggg tgatgacccg cgcgccgaga tcggcggcgc cggtgcgttg
     2221 ccggccgcgt cgtcgaccca tcaccaggtc cagccgtatg ccgtgacgca cccgacacgc
     2281 caaccgcacc tcataccgca aaggcttttt catgtccttc gacttcaagg tcaaagacct
     2341 cgccctcgcc gaatccggcc gccaccagat ccgcctcgcc gagcacgaga tgcccggcct
     2401 gatgtccctg cgcaccgagt tcggcggcgc ccagccgctc aagggcgcca agatcgccgg
     2461 gtcgttgcac atgaccgtgc agaccgccgt cctcatcgag accctcgtcg ccctcggcgc
     2521 cgaggtccgc tgggcctcgt gcaatatctt ctccacccag gacgaggccg ccgccgccgt
     2581 cgtcgtcggc cccgacggca ccaccgcgaa cccccaaggc gtgccggtgt tcgcgtggaa
     2641 gggcgagagc ctgccggagt actgggagtg caccgacgcc atcctgacct ggcccggcgg
     2701 tgacggcccc aacatgatcc tcgacgacgg cggcgacgcc accatgctcg tgcacaaggg
     2761 caaggagtgg gagcgcgccg ggcaggtccc gccggtcacg gaggaggact ccgaggagtt
     2821 cggcgtcttc aaggaactcg tccgcgccac gatggcggcc gacccgcaga agtggacccg
     2881 aatcgccgac ggcatcaagg gtgtcaccga ggagaccacc accggcgtgc accgcctcta
     2941 ccagctcgcc gaggccggcg aactgctctt cccggcgatc aacgtcaacg actcggtgac
     3001 caagtcgaag ttcgacaaca agtacggctg ccgccactct ctcgtcgacg gcctgaaccg
     3061 ggccaccgac gtcctcatcg gcggcaaggt cgccgtggtc gccggctacg gcgacgtggg
     3121 caagggctgc gccgagtcgc tccgcggcca gggcgcccgg gtcatcgtca ccgagatcga
     3181 cccgatctgt gcgctgcagg cggccatgga cggctaccag gtcgcccgcc tcgacgaggt
     3241 cgtcggcgcc ggcgacatct tcgtcacggc caccggctgc ttcgacgtca tcaccgccga
     3301 gcacatgcag gcgatgaaga acaaggcgat cgtcgccaac atcggtcact tcgacaacga
     3361 gatcgacatg gccggcctgg ccaaggtcga gggcgtcacc aagaccgaga tcaagccgca
     3421 ggtccacgag tggaccttcg ccgacggccg ctcgatcatc gtgctgtccg agggccgcct
     3481 gatgaacctc ggcaacgcca ccggccaccc gagcttcgtg atgtccaaca gcttctccaa
     3541 ccagaccatc gcccagatgg agctgttcgc ccaccccgag tcctattgcg acgaggacgg
     3601 caagcccacc gtcaccacgc tcccgaagca cctcgacgag aaggtcgccc ggctccacct
     3661 cgaggccctc ggcgtcgagc tgaccgagct gaccaaggag caggcggagt acctcggcgt
     3721 cgacgtcgcc gggccgttca agccggagca ttaccgctac tgacgctgtg ttctcgccgc
     3781 ggtcgcggcg aggcgtcggg ggaggagatt cgccgctcgg tcgcagagcg acgctcgtgg
     3841 ctcaactccc cctcccgcct cgctgcgtcc gcggctggca ccgtccaccg tcgcgaagca
     3901 actgatccgc ctggattagg gccggcgacg gacaccgcgg tgcctgaggg caacctcccg
     3961 gtgtaccgcg tccactcacc gcatgcacta cccatgcact accgatgggt agccccgtgt
     4021 ttggtcgggt ctcttgtctc gatatccggc ggcgccagcc cactcggcgc tcgcggctcc
     4081 cggcggcgct cgggcgggcg gcccggcgcg gcgagcgggg gaggctcgag tcacggatta
     4141 cacttggccg aattgttatc ggggcgcaaa gggagtgctt gtgaagggtc gcgtgctgat
     4201 cgtcgatgac gacctggcgc tggcggagat gctcggcatc gtgctgcgtg gcgaggggct
     4261 cgatgtcgtt cacgtcgccg acggttcggc ggcggtgggg gcgttccggg accacaagcc
     4321 cgacctgatc ctgctcgatg tcatgctgcc gggcctggac gggatccagg tatgccgtcg
     4381 catccgcgcc gagtccgggg tcccgatcgt catgctgacg gctcgcaccg acaccgtcga
     4441 catcgtcgtc ggcctcgaat cgggcgcgga cgactacgtc gtcaagccgt tcaagccggc
     4501 cgagctgatt gcccgggtcc gggcgcggct gcggcgcggc gaggaggccg agccggagcg
     4561 actcgagatc ggggacctgg ccatcgacgt cgccgggcac tcggtgaccc gcgacggcaa
     4621 gcagatcccg ctcaccccgc tcgagttcga cctgctggtg gcgctggcgc gcaagccgtg
     4681 gcaggtctac acccgcgagg tgctgctcga gcaggtctgg ggctaccgcc acgccggcga
     4741 cacgcggctg gtcaatgtgc acgtccagcg gctgcgcagc aagatcgagc acgaccccga
     4801 ggaccccaag atcgtcgtga ccgtccgcgg cgtcggctac aaggccggcc cgggcgggtg
     4861 accgcgtccc gccggccatg aggcggctcc tgcgcgggct gctggcgccc tggcgccggt
     4921 ccctgcaact gcgggtcgtc gcctccaccg tcctccttgg catcctggcg atctcgctgc
     4981 tgggtgccta cctgttccag aaggtcgccg acgggctcac cgaggaccgc atcggctatg
     5041 ccgaacgcga ggccgcggtg ctggccgccc gcgcacagac ggccttcgac cagaccgatg
     5101 ccaccgggga catctccgag ttcaacaact tcgccagcgg cgccatcgtg gccctggcac
     5161 cgccggccaa cgacaccagc cggtatgtcg tcttcctccg ctcccaggac aacaccaacg
     5221 ccgtccgtct cgacaccgtg ctcaccggtg gcctgcgcac ctcgccggtg cccgtcgcgc
     5281 tgcgcgaggc catctcctcc gatgtggggc gccagcagcg catggtcatc gaactcgacc
     5341 gctccgaact cgtcggcgag ccgtcggcca actgggcccg ggcccgcata ccggccgtga
     5401 tcgtcggcca gcagatccag gtgccgaccg ccggccccta cgacctgtac ttcatctacc
     5461 cgctcgatcg ggagcaggcg acgatggatc tgatccgccg gctcttcctc atcggcgggt
     5521 tgctgctggt caccctcgtc ggcggtatcg cctggctggt gaccaggcag gtcgtcaacc
     5581 cggtgcgacg ggcggccggg gtggccgagc ggctggccgc gggccacctc aacgagcgca
     5641 tgagcgctcg cggtgccgac gacctggccc tcctcggcac ctcgttcaac ggcatggccg
     5701 accacctgca gagccagatc cgccagctgg aggacctctc ccgggtccag caggtcttcg
     5761 tctccgacgt ctcccacgag ttgcggaccc cgttgacgac gatccgcatg gcagccgacc
     5821 tgatccacga gggccgcggg gagttcagcc ccgccctggg gcgcagtgcc gaactcctca
     5881 acaccgagct cgaccggttc gaggcactgc ttgccgacct gctcgagatc agccggttcg
     5941 acgcgggggc cgcggccctc gaactggacc gggtcgacct cggtgcgctc gccgaacgcg
     6001 tcgtccaggc cgccgccccc ctggccgaca agcgcggcag caccgtcacg gtcgacgtgg
     6061 cgccgggcgg tgtcttcgcc gatgccgacg cccgccgggt cgaacgcatc gtgcgcaatc
     6121 tcctggtcaa cgccatcgag cacggtgagg gactgccggt gaccatccgg gtcgccgcca
     6181 actccaccgc cgccgccatc gccgtccacg acctcggcgt cggtctgcgg cccggcgagg
     6241 cgtcgctggt cttcaaccgc ttctggcgcg ccgaccccgc gcgggcccgc accaccgggg
     6301 ggaccggcct cgggctggcg atctccctcg aggacgcccg cctgcacgac ggctggctgc
     6361 aggcatgggg tgaagcgggc cgcggatcgg ttttccgctt gaccctgccc cgggtcgcgg
     6421 gcgagccgat cggtgaatcg ccgctgccgt tggcgcccga gcccaccgac gtcgatgcga
     6481 cgacccggct gcggccggtc cgcctgacgg agacacgatg accgcccgcc gggcccgagc
     6541 gctgctcggc gccctcctcg cgctcctgct gagcggctgc gtcggcctgc acgccgaccc
     6601 ccgcatccaa cccggactcg gcgtcggacc ggccgaccgg gacgaggtcg gcttcatccc
     6661 gccgggtccg caggacgggt cgtcgccgga agccgtcatc cgcggcttcc tgcgggccgc
     6721 cggcgtctcg gggcaagggg tcgccgtggc ccggacctac ctgacccaga gcctggccgc
     6781 gtcctggaac ccggaggatc gcgccgtcgt caccgaagag gggatcgagg ccttcctcga
     6841 ccaggtcgcc accgacacct actcacttag cgggaccctc accggcaccg tggacgccca
     6901 gggccgctac gagccggccg cctcggcgac ccgcaccacg gcccgaatca acgtcaccca
     6961 ggtggcgggg cagtggcgga tcagcgggtt gccggacggg ttcggccggt ggctgtccaa
     7021 ggccgccttc cgccagctga tgacgccata cgacttctac tacgtcggac ccgacgacgc
     7081 cctggtcccc gacgtgcgct acgtcgccac cgacaagctc gccacccgcc tcacccgggg
     7141 cgaactcggg cccgtgcccg cctacctcgg cggagccgtc cgcaccgatc tcggtccggg
     7201 agttcggctt tcggtcgacg ctgtgcccgt cgagggcggg gtcgccgcga tcgatctggc
     7261 cgccgcgcac gtcagcgccg acccggccgc gcggcggcgc atgtgggccc agctggtcgc
     7321 cacggtgacc caggcgccca atgtgtcgag cattgccatc accgtcgaag gcgccggcct
     7381 ggacctcggc gggctgaatc cgcccgtgtc gggcatcgac gccctcggct tccaggagac
     7441 cgccgcgccc gtgggcgtgt tgcccctcct gcggctcggc cgcggcctcg tgcccaccga
     7501 cccggcccgc atcgggcagc ccgaccaagg cggtatcgcc gagcaaccct ccgcctaccc
     7561 tcgcatcgag cgaacgttca ccgaactcgc cctggccttc acggggcagg aggtggccgc
     7621 ggtcgcggag ggcgaactcg cccggttccg cgccagcgcg ggtgcctcgg tgccgaactt
     7681 cggcagcaac ctcaccggcc cggcatacga ccgcaaaggc ttcctctggg tgggcggccg
     7741 gatgagcggc ggacgacacg acggcgctgt ctgggtcgtc gactccggga tctggccgcc
     7801 ggccaacgcc ccggcccgcc ggatcaccgc ctcctggctg gccggccgcc gcccggtatc
     7861 gatcgcgatc gcgctcgacg gcgcccgcgt ggccgtcatc agcaccgacg acaagggtcg
     7921 cgacgcacga ctggacatcg cggggatcgt ccgcgacgac ctcggcgcgc cggccggcct
     7981 cgccgagagc ccgctgcggc tggcgcccat gctctcgacg ctgcgcgatg tcgtgtggct
     8041 gagcgagagc gatgtcgcgg tgctcggcgg cgagaccaag caggccctgc ggccgtatgt
     8101 cgtgaccctg ggtcaacagg tcacccccct cccggaggtg aagggcgcgg agcgcatcgt
     8161 caccctgggc ggggagcggc ggctgttcgt gcgcaccgcg gacgagctct acatgcgggc
     8221 cggcggtcgc tgggcggagg tcggcgtggg cgacgacctg gtcgtcccgg gtcgctgagg
     8281 caccccccac gacgttgtcc acacccggcg ttgtccacac ccggcacacc cggggtggcc
     8341 cggtcccgcc cgggaatggt tgcctggatc gtatgcggcg gcggatcgac atcggcgggc
     8401 tcattgacct cgccctgccg cgcgcctgcg ccgggtgcgg cctgcccggc ccgagcgtct
     8461 gccccgactg cgtcgaggac atcgccgcca cgaccttcgc cggcggcccc aagcccatcc
     8521 tgccgtggcc ggcgccgcgc gggctggggc cggtatgcac ggccggggcc tactcgggtg
     8581 ccctcgcctc cgtcatcgcc gggcacaagg acgccggccg cgacgacgtg cgcagcctcc
     8641 tcgcgctgct gctcgcccga gccatccggg cggcgcgagt gccgccggac gccgtggtcg
     8701 tggggctgcc gagttcgcct gcggcaaccc gacgccgcgg tgatgccccg gtcgcgcggc
     8761 tcgtcgcgca cgccgcggca gccggccagc tgcccgccat cgacgcgctg cgggtcagcg
     8821 ggcgggtgcg cgacgcggtc ggcctcagtt cggcgcagcg ggcacgcaat atctccggcg
     8881 tgatgcgcgc ctcccgtgac gtcgccggcc aggtcgtcgt cctcgccgac gacatcgtca
     8941 ccaccggtgc cacgatgcgc gaggccgtcc gggcgatcga agccgccggc ggggtggtcg
     9001 tggcccgtgc cgcgatcgcc gccacgcccc ggcgattccg cgaagagcaa ggcgaatccc
     9061 tgcacaacgg gaccgaagtg agctagcgtc ggtacatgag ggccataccc cgcaccgacg
     9121 ccacgagcgg agaggggtga tgcgactggc tggccacaaa aaccgttccc gcgcaacagg
     9181 tcccgcttgt ccgtaggacg cgatccggac agcaggcacg gcgggatcca ccgcagcacg
     9241 atcgcatcac ccgacctcaa cgaggagaac ccatggatgt tgtcatctcc ggccgccacc
     9301 agaccgtcag cgatcgattc cgtgatcacg tcacggagcg agtgggcaag gtcgagcaac
     9361 tcgcgccgcg cgccctgcgc gtcgaggtcc tcgtcagtca cgaacccgcg cgcagcaagg
     9421 ccaaggcgac cgagcgcgtc gaaatcacct gtcacgcccg cggacccgtg gtgcgtgccg
     9481 aagcgaccca tgacgacaag tacgctgcct tcgagcaggc gatggacaaa ctgctcgagc
     9541 ggctgcgccg cgccaacgac cgccgccggg tcgcccgcac ccgaggcaag aagccggccg
     9601 aggtgccgct cgaggcggtc ctcaccgagc ccgcccccgc cctggaggtc gacgacgaac
     9661 gcgacctgtt cggcgccatc ggcgacagcc cgatcgaggt ccgcgagaaa gtccaccaga
     9721 gcgccccgat gacgctcgcg gaggcgatgg ccgagatgga actcgtcggt cacgacttct
     9781 tcctcttcca ggacagcgag acaggcaagc cgtcggtcgt ctatcgccgt catggttgga
     9841 actacggcgt catccacctc gaggtcgccg cccccgcgga ggccgctagt taggatcacc
     9901 tgatgacgat gcgcctgtcc caagcacagg cgcgacgaat cgcgttggcc gcccaaggat
     9961 ttgggcggcc gcgcggcgtc cgggagccga cgatgcgcga catccagcgg gtcatcgaca
    10021 cggtcgggtg catccagatc gacagcgtca acgtggtggc gcgcagtcag tacctcccgt
    10081 tcttctcccg cctcgggccg tatgacgtgt ccctcctcga ccgagcccgc gaccgggcgc
    10141 cgcgccggct cgtcgaatac tgggcgcacg aggcgtcgct gatcccgccc acgacgtggc
    10201 cgctgctcga cttccggatg cagcgcgcgc tgtccgattc gtggggcggg atgcagagcg
    10261 tcgctcgcac ccggcccgac ctcgtcgcgg ctgtgctcac ggagatcgag gcccgcgggc
    10321 cgctcacgtc gcgccagacc gagatcgccc tcgaacacga cgtgccgcga tcgaaggagg
    10381 gctggggctg gaactggtcg ctcgtgaaga acgccctcga acacctcttc tggggcggcc
    10441 agatcggatc gtccggccgc acaacgcaat tcgagcgcaa gtacgacgcc ctccaccgcg
    10501 tgctgccccc ggcggtgcac ccccacgcga tcgaggcgag cgaccggccg ccggtggaga
    10561 cggcataccg gcagctcgtc gagatcgccg cccgcgcgca cggcgtggcg acagtgcaat
    10621 gcctgcgcga ctacttccgc ctacccgtca aggcgaccca gcccgcgatc gaccggctcg
    10681 tcgccgacga cgtcctgatc ccggtcgagg tcgaagggtg gcggcgggcg gcatatctgc
    10741 accgcgacgc gcggcggccg cgacgggtcg aggccagggc cctgctcagc cccttcgact
    10801 cgctggtctg gacgcgggac cgcgtctccg cgctcttcgg cttcgactac cggctcgaga
    10861 tctacgtgcc cgcgccgaag cgcgtgcacg gctactacgt gctgcccttt ctgctcgggg
    10921 acgaactcgt cggccgcgcc gatctcaagg ccgaccgcgc cgccggcgtg ctgcgcgtcc
    10981 agcggctcac ctgggaacca gggcgcggcg ggtcgagcga ccggagcgag ttggaggccg
    11041 aactccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg gccacggcat
    11161 acacgccgct ggtctcgaca cagtccgtgt tgccgctgag cgagaaccag atctgtgccg
    11221 aatccgccgt gaagtcgatc cggtcgtcgc cgaccccgag cggggtgacg ccgtcgtccg
    11281 gccagatcac ggtctgcacc acccgcttga ctccggtggc ccgggtgatg accaccaggc
    11341 ggtgagagcc gcatccggcg gcctgcttgg tgcgttggac ataagcgaag tacgtgccgt
    11401 cggccgacca ggcgggctgc gaattgccgc aggtcgtggc gccgtcggtg gtcaaccgct
    11461 tggccacacc gcccgccgct gggacggcgt agatgtcgcc cttgccgttg atcaccttga
    11521 cgaagccgac ctgactcggc ttgccggtga cgggcatcca attgggctgc caactccggc
    11581 cgaccgacac cgtcttcttg gccgtgccgt cgggcttcat cacgacgata ttgcgagcgc
    11641 cggtggcgta ggcgagtttg gtgccgtcct gcggccagcg cgggtggtca ccgacaccgg
    11701 gtgtcagccg cttcgtcatc acctggccgg cgacgagccg atcgctgtag atgctgtggt
    11761 cgagaccgac gtacgccagg gcgccgggcc ggccggggga ggtggcgtgg ctaccggtcg
    11821 cggcgagtcc cacggccgcc gtcacagcca gcgcgctcgt caggagtctc gcgggagtgt
    11881 tcatgagctt tccctctctc gtcggggggc ggccggtggc ccccctttcg atggtaggga
    11941 ctcggcaggt ggccggcgac cggttgtgag ctaccgatca ttggcgcacg cgttcccagt
    12001 gatcggcggg gcccaccgag ccgccggggg cggtcgcctg ggcctcgtgt ccccagcggg
    12061 tgaagccggc cgattcgagg atccggttgc tgacggcgtt gtcggcggcg gtctcggcga
    12121 cgaggcggcg taggccgagc ccgtcgggcg agagggcgta cgccgtggcg agccgcgttg
    12181 cgcgggaggc gaatccgcgg ccgcgggcgc tcggcttgag gaagtagccg agttcggcgg
    12241 tgtccccgtc ggtcatcgac ccatggtgga cgaagacgag catctcaccg accggctcgt
    12301 cggtggcggg gtccgcaatg caccagttga cggaggttcc ctgcgacatc agctcccgcc
    12361 gccgcatcag ccaggcctcg aaggtctcgg ccgtggggat cgcgcgctcg ggcacgtgat
    12421 ggctcggatg gtcgtgcggc tccgcgaccg cgaggtcgtc gtcgcgccac ccgcgcagcc
    12481 gtatgccgtc gctctcgagc gtcgggggct cgaaccacgg tgccttcggt ttcgtgaggt
    12541 cgtcgtccgc gcccacgctg gccgcccagg cgtcgagggc gaccgcctcc gggtcgccgg
    12601 gtgccggggc gagtttctgc ggcagcagcc cgtggtgctg gaatccgcac gcccacgcca
    12661 cgcgccacga ggcgaagttg ccgcgggcgg cataccagta gaggcggtgc ccgccgaggt
    12721 cgccgaagta gtaaccggcg agcagccgga cggcgcgcga catcaagccc cgaccgcggc
    12781 cggccggatg cagcccgaag cccacctcgg cggttccgcc cggcccgtgg cgcaggtcga
    12841 tcgtgcctaa gtactcgtcg ggatcggcgg cgccggcgat cgcccagtgc aggttccccg
    12901 tgcccgcgtt ccagccgtcc tcgatcagcg tgcggaactc ctgcgcctgc tgcattccgt
    12961 atggccgcgg cacggtcgtc caccgcatcg aggcggggtc gttcgactgg gccacgatgc
    13021 cctcggcgtc cgacggccgg tgtgcgcgca gcagcaccac gccatcggtg agggtgggga
    13081 ccacgtcggg gaacgccata ccgcccatcc tttcctggcc aggggcggag gttccagtcg
    13141 attgccggta tgccgcgccg gccggaatca tccggccgga accatcccgg cggggcgagg
    13201 cgttggggcc acggtggagg ctcgattagg ctgagcaggt tccgagagct acgtcaggag
    13261 aagtcgcgtg cccaagttcg tcgaaaggct gctgcgtgcc ggcgaaggcc gcaccgtcaa
    13321 gcggctggag agcatcgccg cgcaggtcaa cgccatcgaa gaggacttcg agaagctcac
    13381 cgacgccgag ctgcgcgccg agaccgacgt cttcaagcag cgcctcgccg acggggccac
    13441 cctcgacgac atcctccccg aggccttcgc cgccgtccgc gaggcgagca agcgcaccct
    13501 cggcaagcgg cacttcgacg tgcagatcat gggcggcgcg gccctgcacc agggcaatgt
    13561 cgccgagatg aagaccggtg agggcaagac gctcgtcgcg accctgccgt cctatctcaa
    13621 cgccctgacc ggcaagggcg tccacgtcat caccgtcaac gactacctcg cggcatacca
    13681 gtccgaactg atgggccgca tccaccgcat gctcggcatc gagaccggct gcattctctc
    13741 ggacatgacg ccggagcagc ggcgcgccga atacgccaag gacatcacct atggcaccaa
    13801 caacgagttc ggcttcgact acctgcgcga caatatggcc tggtccaccg acgaactcgt
    13861 gcagcgtggc cacaacttcg ccatcgtcga cgaggtcgac tcgatcctca tcgacgaggc
    13921 ccgc
//
LOCUS       DAOPJG010000078        13848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4634_length_13848_cov_8.825854, whole genome shotgun sequence.
ACCESSION   DAOPJG010000078 DAOPJG010000000
VERSION     DAOPJG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13848
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4634_length_13848_cov_8.825854"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="PKU75_08690"
     CDS             complement(<1..1250)
                     /locus_tag="PKU75_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF80993.1"
                     /translation="MDPIRNPYAPGAGQRPPELAGRDEQLTQFDIVLQRVAKGRPERS
                     LILTGLRGVGKTVLLNALRGQAVRAGWGTGKFEARPDQALRRPIAAAAHVAVRELGHT
                     QSDQVDHVLGVVKSFAQRETTPTAKLRDRWNPGIDAPAVPGRADSGDIEIDLVELLTD
                     IGGLAADLGRGIGIFIDEMQDLQPEDVSALCAACHEISQSSLPVIVVGAGLPHLPAVL
                     SASKSYSERLFRYARIDRLDRAAADAALSVPAGEEDAAFEPDALAEMYAVTGGYPYFI
                     QAYGKEAWDLAPASPITADDIRVAVPRAESELAVGFFGSRYERATPAERDYLRAMAAV
                     ALVDADGDPVGAPKDSGEAEAYDPATTTELDAVLTSDVAKWLGKQPQSLSPARDALLK
                     KGLVYSTERGRVAFTVPHFGRYLR"
     gene            complement(1361..2038)
                     /locus_tag="PKU75_08695"
     CDS             complement(1361..2038)
                     /locus_tag="PKU75_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPF80994.1"
                     /translation="MEGPDRLHRWAVLDMLAHLAGAAECAVRPVAMARHWGYAGWRAA
                     RRPVEFVDHMCASQIRSRKDLSTAELEVDLRRWASQAPEKGAGRPRLVRGIPVPAVAG
                     LPRGARVSNFVDVISIRDVWMHRIDIARAIGRPRRPITVAEPEAVRQVVRDLDIEWTS
                     PAIDLVLTGPGGGAWRVGDGEPIAEITEDAGALMRLLSGRSDECELRFEGSATGSQLL
                     RAARVLF"
     gene            complement(2155..2511)
                     /locus_tag="PKU75_08700"
     CDS             complement(2155..2511)
                     /locus_tag="PKU75_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF80995.1"
                     /translation="MGEVEERRGRVTDAIVMFWRPIPNWQPSPGAFAAAVDVIATTTG
                     LLGQAQLIGLLRNDINLEEVVQVLAALIAGVISQQLSTEPGTDAVTGRVSRYADSIAT
                     MVVRHYGADPTARSIP"
     gene            complement(2754..3092)
                     /locus_tag="PKU75_08705"
     CDS             complement(2754..3092)
                     /locus_tag="PKU75_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HPF80996.1"
                     /translation="MSERQTPIPRDFWADDTGLARERLDLSWGRTLLAVMTVTGLFLR
                     WLPVHGPAAIIPVVLGVGAGLGLMHLRHNRRREMFAGEGDAVREVLGLTAMVLIVGAS
                     ALALFASGLR"
     gene            complement(3097..3495)
                     /locus_tag="PKU75_08710"
     CDS             complement(3097..3495)
                     /locus_tag="PKU75_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HPF80997.1"
                     /translation="MSTQPDTSGEGREGRFERRVLGPGNEPDPRYSLANERTFLAWIR
                     TALTLIGGGVAIEAFAGTALSFVARRGLALALVCLGMLLAAASCYRWVTLERAMRQGK
                     PLPLNPVALVLAAGVTLAALVLAASIAVRG"
     gene            complement(3510..4043)
                     /locus_tag="PKU75_08715"
     CDS             complement(3510..4043)
                     /locus_tag="PKU75_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3806 domain-containing protein"
                     /protein_id="HPF80998.1"
                     /translation="MAWFGRGKGSADEVAEAAAADTSRFPTADESMIDDAGMGPLRPR
                     DRQIGANEQARIDAGLAALAAAGVSVDDLDALGAAYDREYAEWHGTRKGKRPDHDLIV
                     EKYAIGIGEHLARTTDLRWRIVTDVFGTDLALADQKEGDFIVVPTNLVAARWMRTEIG
                     WIPGVVRHLIAVRTDAR"
     gene            complement(4095..4535)
                     /locus_tag="PKU75_08720"
     CDS             complement(4095..4535)
                     /locus_tag="PKU75_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPF80999.1"
                     /translation="MATSKATEEGGFTAEERAAMKERAAELKAEKGGKKKADELQSLL
                     DKIAEMPAEDRVIAERLHALMSKIAPDLAAKTWYGMPAYARDGKPLVFLQNAAKFGTR
                     YCTLGFQDNAALDDGNMWPTSFAITTWNDAVEKKVSALVKKAVG"
     gene            complement(4541..4951)
                     /locus_tag="PKU75_08725"
     CDS             complement(4541..4951)
                     /locus_tag="PKU75_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF81000.1"
                     /translation="MDITIHQSFLPHIDPDESVAFYRDVLGFEVRADVGQGTWRWITV
                     GPAGQPDTAIVLQPPVCDPSISEDEKRTISELMAKGNYAGVNLATPDVDAAFNEIQAK
                     GVDVVQEPIDQPYGLRDCAFRDPAGNLIRLQQAG"
     gene            complement(4957..5418)
                     /locus_tag="PKU75_08730"
     CDS             complement(4957..5418)
                     /locus_tag="PKU75_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF81001.1"
                     /translation="MATADEQRLKDLAQLRRVRDRIDRDYAQPLDVEALARGANMSAG
                     HLSRQFKAAFGEALYSYLMTRRIERAMALLRRGDLSVTDVCFAVGFSSLGTFSTRFSE
                     LVGMPPSAYRDQAEEVAANGVEAHGIPPCVAKRVTKPVRNQEAPPGLWPLA"
     gene            complement(5442..7019)
                     /locus_tag="PKU75_08735"
     CDS             complement(5442..7019)
                     /locus_tag="PKU75_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HPF81002.1"
                     /translation="MADDTPSDTPAQAPPPAVAGDGPEALEAPGEVTTTVTTTTQAKT
                     KPVAPRDDLVTTRHTLKVGRKSLHYTARAGRIVLRDEVTEDGVFRGLKPRAEMFVTAY
                     TLDETDPTTRPIAFVFNGGPGSASIWLHMGLLGPRRVLMGDVGDLAPPPYGLADNPQS
                     LLTECDLVFIDPVSTGYSRAVEGGKAGEYHGFTKDVESIGQLINLWVTREKRWLSPKF
                     VIGESYGTLRGTALTEHLQSRYGMMLNGLVLISSVLDLASIDFEKQRNDRAHALYLPF
                     YAATAHLHGKHGRRSRDSVVAEAQAYADRDYPYVLSQGARLPAKERAEAVATIARLAG
                     LSEDYVDRADLRIEHWRYFGELLRDQRLTVGRLDSRFTGPAASAIAEEMDADPFFDAS
                     DGAYTAAFNHYIRDELDYVNDLPYQRLAEEVHPWSYKEFEGRPIDVSPQLERAMRKNP
                     HLLVHVAFGVYDGATPPAAAEQVLALLRLPEHLRANIEKCYYDAGHMMYVHEESRLKQ
                     SADLAGFVRRASRLTTP"
     gene            complement(7047..7907)
                     /locus_tag="PKU75_08740"
     CDS             complement(7047..7907)
                     /locus_tag="PKU75_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPF81003.1"
                     /translation="MPELPEVEALVEFLRSRTKDLAVVGVEVGSITVLKTFNPPPEAL
                     VGAPVDAVSRFGKFIDMDCGGTHLIFHLARAGWLRWSDALPATVIRPGKSPIALRVRL
                     DDGSGFDLTEAGTKKRLAAYVVGSPEQVPGIASLGPDPLDPGFTEQEFTAILHGRRAQ
                     IKGVLRDQGIIAGIGNAYSDEILHVAKMSPFAIAGSLDADAVARLYEAMRETLAAAVS
                     AASGKPAAEVKDAKRAGMRVHGRTGEVCPECGDVVREVSFADSSLQYCATCQTGGKPL
                     ADRRTSKFLK"
     gene            complement(7911..8996)
                     /locus_tag="PKU75_08745"
     CDS             complement(7911..8996)
                     /locus_tag="PKU75_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HPF81004.1"
                     /translation="MAKPHAAAIVEDRWNRAKAGLLTRRGWGAQVVPYPGYGSATQAR
                     VLARLLLSKRPDATEPDEVSELVRSRAFSGRRGWRSFVSAPAVNVPVSVTVGERTVHT
                     RTDRSGLLDVTVTDHGMPPGWQPVRITAPGTQEAEAHVRIVADDVTFGIVSDIDDTVI
                     RTMLPRPMIAAWNTFLVNEGGRTAVPGMATMYRELLEEHPGAPIVYVSTGAWNVVPPL
                     TRFLRDKGFPAGPLLMTDWGPTNTGWFRSGRDHKRACLHRLARELPSIEWVLVGDDGQ
                     HDPAIYGEFAEQRPERVRAIAIRRLSATEQLLSHFTPLAQDELGSARRKLDTVPICRA
                     SDGYGLLRLLKVALGRLPIPSDVVVEE"
     gene            9025..9687
                     /locus_tag="PKU75_08750"
     CDS             9025..9687
                     /locus_tag="PKU75_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPF81005.1"
                     /translation="MPPPEHPLAAILRDAAAGRWPADDGGWSHLPGWYAGVGAIVALT
                     GHAFVCSDRQLDRDRLDALGCNGFGGATLPQVVLAVAGRRGWVDCLDALLVGTGRGRE
                     PAPLVPRPDLSAHPRAVNARRSRHATEVLGYADPRRRDVATVARGLAGMTEIGVEAAA
                     PGTGSRLFADVLDSRPVGEVVVAGVAPGNARSLRSALAAGFAIIGSVQIMTVPTAHRG
                     AT"
     gene            9684..10868
                     /locus_tag="PKU75_08755"
     CDS             9684..10868
                     /locus_tag="PKU75_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81006.1"
                     /translation="MTERQAAEPLRINTMGCRWEVDISRLEAPLAQRLRELWAACSDW
                     RPDRSEPDGFPEVLTAYHAGTSDRPDGSVIALQPDVESAPYAFSGAITLRCIERQAGR
                     MTLLHSAALALPDSGATVILIAESGTGKTTAARRLGERLGYLSDETAVIRGDLTLIAH
                     PKPLSIIPEPGRPKIEVAPEEIGLLPAPDQPWLAAAVLLSRSPDVAAPVLEPVDVFEA
                     LAEVIPQSSSLPLQEQPLDTLAGVLTAGAGCFRLRYSEIDAAADLVVDVLRSVSRGEQ
                     RSRTWQVVTPPQDQRTGPWVASPAEEGELPPGGRVVRAPWRDALADEDGDVFALNGPR
                     PVRLMALGASIWRGCAEPRTVEEIRDQIVAEHGPHPDADSLVRQAIATLADEGLVRFV
                     PA"
     gene            complement(10886..11809)
                     /locus_tag="PKU75_08760"
     CDS             complement(10886..11809)
                     /locus_tag="PKU75_08760"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPF81007.1"
                     /translation="MTLPADRPFTEVARSREVLDFLPWALAIAVGCLVAASVLLIPIL
                     THARRAGRDNQSTITARNLLPLGADPTARVNDRVRDAVVQFDLPRDLRPEQMAALLHD
                     GDPERAIAAALLDLQAAGVIDLDEDDRATVRRDADTSGLSDFQQRLLRIIGGRNTRFE
                     WRDWALGALSQDVASRRWHTGRPDYVSYTPRLYASFAGSIAAIMILFSTNLLLGGYWT
                     YAIALYAAALLLLLLAWAFCYSARKRPNRTATGRALAEQLAGFARAVAATGEERHTDN
                     WDAVRVALGVGDRSLRRLVRNIPTEPPTPPR"
     gene            complement(11806..>13848)
                     /locus_tag="PKU75_08765"
     CDS             complement(11806..>13848)
                     /locus_tag="PKU75_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81008.1"
                     /translation="GTGGFTWSDVSPDATPYLWHLLGGGSSSTNSVRSVNPNTCPVDG
                     WLSLSAGQRAAAPTADGAATRNLKEPCPPLPAVTVTGSHATVEGWAELEKAAASLRFE
                     ADPGLLGDQLAGAGIPAVAIGPGAALGLAQSSGEVAAYRPVDWATATLDSVHAALGDA
                     KVALIDAGAVRDPGDLAAGEASPGTSVADQVRAVDDRIATVAAGLDDQDTLIVAGLSD
                     AGMGSRLRPVVVTGPAYGPGQLSSPSTRIPDLMQSSDLTVTLLRLAGIDTTDLGLGGA
                     LLDTEHRPNDAAAVERRHLALTDVDNASHEIHPLVPIFFYGLILTQLAIYLFTWRVWR
                     REGATLAQRTRAIRLVHIVGVLAASVPAATFLANLLPWWRVSHPLPAIVGAVALFVAL
                     IAGLAFLPPWGRHLMGPVAVVGGITMAVLAIDVMTGSRLQQSSLMGLQPVVGGRFYGM
                     GNVTFSIFATASLMLAIAAAHQALRRWGRWAAAAVVAAIGLLTVVIDGNPAWGADGGG
                     PPAYLPGLAYFLLALLGVGMTWRRGAIIALVTAGLFLLVGFLDSLRPAASQSHLGRFF
                     DKLGTGGALDIVIRKAEQNWGILISSPLTVLVPFGLVFVIYVLLRPTSWGSRSLAGAF
                     ERVPTLRAGLVSWLIVMTIGFFINDSGVAIPAVGATVAIPLVVAIASRTLLEEADA"
BASE COUNT         2090 a         4810 c         4913 g         2035 t
ORIGIN      
        1 tcgcgcaggt agcggccgaa gtgcgggacg gtgaaggcga cgcggccacg ttcggtggag
       61 tagacgaggc ccttcttcag cagggcgtcg cgggcggggg agagggattg cggttgcttc
      121 ccaagccatt tcgcgacatc ggaggtgagt acggcgtcga gttccgtggt cgtcgccggg
      181 tcgtatgcct ccgcctcgcc actgtctttt ggcgcaccga cagggtcgcc atccgcgtca
      241 acgagcgcca cggcggccat cgcgcgcagg tagtcgcgtt cggcgggggt ggcgcgttcg
      301 tagcggctgc cgaagaagcc gacggccagc tcggactcgg cgcgggggac cgcgacgcgg
      361 atgtcgtcgg cggtgatcgg gctcgccggc gcgaggtccc aggcctcctt gccgtaggcc
      421 tggatgaagt atggatagcc gccggtcacg gcatacatct ccgcgagtgc gtcgggctcg
      481 aaggccgcat cctcctcgcc cgccggcacc gacagggccg cgtcggctgc cgcgcggtcg
      541 aggcggtcga tgcgggcata ccggaaaagc ctctcggagt aggactttga cgccgacagc
      601 accgctggta ggtgagggag gccggccccg acgacgatga ccggcagcga gctctgcgag
      661 atctcgtgac acgcggcgca cagggcggag acgtcctccg gctgcagatc ctgcatctcg
      721 tcgatgaaga tgccgatgcc gcgcccgagg tcggcggcca gcccgccgat gtcggtgagc
      781 agttcgacga ggtcgatctc gatgtcgcca gagtccgcgc ggccgggcac cgcgggtgcg
      841 tcgatgcccg ggttccaccg gtcgcgcagc ttggcggtgg gggtggtctc gcgctgggcg
      901 aaggacttca cgacgcccag gacgtggtcg acctggtcgc tttgggtatg cccgagctcc
      961 cggacggcga cgtgcgccgc ggcggcgatc gggcgccgga gggcctgatc tggtctcgcc
     1021 tcgaacttgc cggtgcccca gcctgctcgg acggcttgac cgcgaagggc gttgagcagc
     1081 accgtctttc cgacgccgcg caggccggtc aggatgaggg agcgttcggg gcggcccttc
     1141 gcgacacgct gcagcacgat gtcgaattgg gtcagctgct catcgcgccc cgccaactca
     1201 ggtgggcgtt ggccggcgcc aggggcgtag gggttcctga tcgggtccac gatcggactc
     1261 tatgtgtcct tctagcggaa atcgaagagg aatcaagaca aagctatcgg cgtgtcgaca
     1321 tcgcctaccg taagccgcag cgagcagtcg gcatcccggt tcagaagagc acgcgtgccg
     1381 cacgcaacag ctggctgccg gtcgccgacc cctcgaagcg cagctcgcac tcgtcggaac
     1441 gtccggacag caggcgcatg agggctcctg cgtcctcggt gatctcagcg atcggctcgc
     1501 cgtcgccgac gcgccacgcg ccgccacctg gcccggtgag gacgaggtcg attgcgggag
     1561 aggtccattc gatgtcgagg tcacgcacga cctggcgcac cgcctcgggc tcggccacgg
     1621 tgattggccg ccgcggccga ccgatggcac gggcgatgtc gatgcgatgc atccacacgt
     1681 cgcggatcga gatgacgtca acgaagttgc tcacgcgcgc gccgcgaggc aggccggcga
     1741 cagcggggac gggtatgccg cgcaccagcc tcggccggcc cgctcccttc tccggggcct
     1801 gggatgccca ccgccgcagg tcgacctcca actcggccgt cgagaggtcc ttgcgtgagc
     1861 ggatctgtga ggcgcacatg tggtcgacga actcgaccgg gcggcgcgcg gcgcgccacc
     1921 cggcatatcc ccagtgccgc gccatcgcga ccgggcgcac agcgcactcg gccgcgccgg
     1981 cgaggtgggc gagcatgtcg agcacggccc agcggtgcag tcggtcgggg ccttccactc
     2041 ctcggcggtg aggccgtcga cggcggcgac cagggcggca ttctcggcgg tgagcccggt
     2101 gcgtgccaat tcgggtctgc ggcaggtcgc gggcgcgggc ggtcacggag gtggtcatgg
     2161 gatgctcctt gcggtcggat cggcgccgta gtgccgcacg accatggtcg cgatgctgtc
     2221 ggcatagcgg ctgacccggc cggtgacggc gtccgtcccg ggttcggtgg aaagctgttg
     2281 gctgatgacg cccgcgatga gagcggcgag gacctggaca acctcttcca ggttaatatc
     2341 gttgcgcaag agaccgatca gctgggcctg cccaagcaga ccggtcgtcg tggcgatcac
     2401 gtcgacggct gccgcgaacg cgccgggcga cggctgccaa ttgggtatcg gccgccagaa
     2461 catcacgatc gcatcggtga ctcgcccgcg ccgctcctcg acctcgccca cgccacccaa
     2521 ttctcctaat gccattagga aagttcctgg gcaaaacgac gcgtaagcgg gcgcgtgtga
     2581 tgtgggcgaa cacgccggcg gccaccatcc ggtcggtgcc gctgaggttc ggcacggatg
     2641 atcgggagtc gtccgcgacc caggcctgac gctgggccct gcgctcagga cgtgccttgg
     2701 gcagcgacga cgatcgccga cgccccgcac ccgctcaggt ctccgatgag ccgtcagcgc
     2761 agccccgaag cgaagagggc cagcgccgag gcgccgacga tcagcaccat cgccgtgaga
     2821 ccgaggactt cgcgaacggc gtcgccctcc ccggcgaaca tctcccggcg tcggttgtgc
     2881 cgcaagtgca tcagcccgag accggcaccg acaccgagca cgaccgggat gatggcggcc
     2941 ggtccgtgca ccggcagcca gcgcaggaag agcccggtca cggtcatcac ggccagcagg
     3001 gtccggcccc aggacaggtc gagccgttcc cgcgccagtc cggtgtcgtc agcccagaaa
     3061 tctcgcggga tcggcgtctg gcgttcgctc acggtttcaa ccgcgcaccg cgatcgaggc
     3121 ggcgagcacc agcgcggcca gtgtcacgcc cgcggcgagg accagggcaa cggggttgag
     3181 tggcaacggt ttcccctgcc gcatcgcccg ctccagggtc acccagcgat agcaggaggc
     3241 ggcggcgagc agcataccca gacacaccag ggccagcgcc agcccgcggc gggcgacgaa
     3301 ggacagcgcg gtcccggcga aggcctcgat cgccacccca ccaccgatca gcgtgagcgc
     3361 cgtgcggatc cacgcgagaa aggtgcgctc gttggcgagg ctgtaccgcg gatccggttc
     3421 gttccccggg ccgagcacgc gccgttcgaa acgaccctcg cgaccctcgc cggacgtgtc
     3481 gggctgcgtg ctcaccggat caccctccgt cagcgcgcgt cggtgcgcac cgcgatcagg
     3541 tggcggacca cgccggggat ccacccgatc tccgtgcgca tccatcgggc ggcgacgagg
     3601 ttggtgggga cgacgatgaa gtcgccctcc ttctgatccg ccagcgcgag gtcggtcccg
     3661 aagacgtcgg tgacgatgcg ccagcgcaag tcggtggtgc gggccaggtg ctcgccgatc
     3721 ccgatcgcat acttctcgac gatgaggtcg tgatcgggac gtttgccctt gcgcgtgccg
     3781 tgccactcgg catactcccg gtcgtatgcc gcaccgagcg catcgaggtc gtcgaccgaa
     3841 actcccgctg ctgcaagggc agctaggccg gcgtcgatgc gcgcctgctc gttggcgccg
     3901 atctggcggt cacgcgggcg caggggaccc attccggcgt cgtcgatcat cgactcgtcc
     3961 gccgtgggga atcgcgacgt gtcggcggcc gcagcctccg ccacctcgtc agccgaaccc
     4021 ttgccgcgtc cgaaccacgc catggcccaa agcctagggt gtgtccgccc tcgtggcgcg
     4081 gcaatcggtg gtcgtcatcc gacggccttc ttcaccagcg cgctgacctt cttctcgacg
     4141 gcgtcattcc acgtcgtgat ggcgaaggac gtcggccaca tatttccgtc atcgagagcc
     4201 gcgttgtcct ggaacccgag cgtgcaatag cgcgtcccga acttcgcggc gttctgcagg
     4261 aagaccagcg gcttgccgtc gcgggcgtag gccggcatgc cgtaccaggt cttcgccgcg
     4321 aggtcgggcg cgatcttgct catcagcgcg tgcagccgct cggcgatgac ccggtcctcc
     4381 gccggcatct cggcgatctt gtccagcagg ctctgcaact cgtcggcctt cttcttgccg
     4441 cccttctcgg ccttgagttc agcggcacgc tccttcatcg ccgcgcgttc ctcggcggtg
     4501 aagccgcctt cttccgtcgc cttggaggtt gccatgccac tcaccctgcc tgctgcagac
     4561 ggatcaggtt gccggcagga tcgcgaaaag cgcagtcgcg caacccatac ggctgatcga
     4621 tcggttcctg cacgacgtcg acgcctttgg cctggatctc gttgaaggcg gcgtcgacgt
     4681 ccggtgtggc gaggttgacg ccggcgtagt tgcccttggc catgagttcg gagatcgtgc
     4741 gcttctcgtc ctcgctgatg ctcggatcgc acaccggcgg ctgcagcacg atggcggtgt
     4801 ccggctggcc ggccgggccg acggtgatcc agcgccaggt gccctggccg acgtcggcgc
     4861 gcacctcgaa gccgagcaca tcgcgataga acgcgaccga ctcgtccggg tcgatgtggg
     4921 gaaggaagct ctggtgaatg gtgatgtcca tgacgatcac gctaggggcc acaacccggg
     4981 cggcgcttct tgattcctga ccggtttcgt gacccgcttc gcgacacagg gcggtatgcc
     5041 gtgggcctcc accccattcg ccgccacctc ctccgcctgg tcgcgataag cgctcggcgg
     5101 catacccacg agctccgaga agcgggtcga gaaggtgccg agggaggaga agcccacggc
     5161 gaaacagaca tcggtgacac tcaggtcgcc gcggcgcaag agcgccatcg cccgctcgat
     5221 ccgccgggtc atcagatagc tgtacagcgc ctccccgaac gcggccttga actgccgact
     5281 caagtgtccc gcagacatat tcgcgccacg ggccagagcc tcgacgtcga ggggctgcgc
     5341 gtagtcgcgg tcgatccggt cccgcacgcg acgcaattgc gcgagatcct tcagacgctg
     5401 ctcgtcggcg gtggccacga tgagggtctg tcgcgagatg gtcagggcgt ggtcagccgg
     5461 ctggcccggc gcacgaagcc ggcgagatcc gcactctgct tcagccgcga ctcctcgtgc
     5521 acatacatca tgtgtccggc gtcgtagtag cacttctcga tgttcgcccg caggtgctcg
     5581 ggcagccgca gcaaggccag gacctgctcc gcggcggccg gcggcgtggc accgtcatag
     5641 acgccgaagg ccacgtgcac gagcaggtgc gggttcttgc gcattgcccg ctccagctgc
     5701 gggctgacat cgatcggccg gccttcgaac tccttgtagg accacggatg aacctcttcg
     5761 gcaagccgtt ggtagggaag gtcgttgacg tagtcgagct catcccggat gtagtggttg
     5821 aaggcggccg tgtaggcgcc gtcggaggcg tcgaagaagg gatcggcatc catctcctcg
     5881 gcgatcgcgc tcgcggccgg cccggtgaaa cgggagtcca gccgcccgac cgtcagccgc
     5941 tgatcgcgga gcaattcgcc gaagtagcgc cagtgctcga tccgcaagtc cgcacggtcg
     6001 acatagtcct cactcagccc tgcgagccgc gcgatggtgg caaccgcctc cgcccgctcc
     6061 ttcgccggca accgcgcacc ttgcgagagc acataggggt agtcgcggtc ggcatacgcc
     6121 tgcgcctccg ccacaacgga atcgcgcgag cgtcgcccgt gcttgccgtg caggtgcgcc
     6181 gtggccgcat agaagggcaa gtagagggcg tgggcccggt cgttgcgctg tttctcgaag
     6241 tcgatcgacg cgaggtcgag gacggaggag atcagcacca ggccgttgag catcatcccg
     6301 taccgacttt ggaggtgctc ggtcaaggcc gtcccgcgca gcgtgccata ggactcaccg
     6361 atgacgaatt tcggtgacag ccaacgcttc tcacgggtca cccacaagtt gatcagttgg
     6421 ccgatcgatt cgacatcctt ggtgaagccg tgatactcgc ctgccttgcc gccctcgacg
     6481 gcgcgcgaat agccggtcga caccgggtcg atgaagacca ggtcgcactc tgtcagcagg
     6541 ctctgcgggt tgtccgccag gccgtatggc ggcggcgcga ggtcgccgac atcgcccatc
     6601 agcacgcgcc tgggcccgag caggcccatg tgcaaccaga tgctcgcgct ccccgggccg
     6661 ccgttgaaga cgaacgcgat cgggcgcgtg gtcgggtcgg tctcgtcgag cgtgtaggcc
     6721 gtcacgaaca tctcggcccg cggcttcagg ccacgaaaga caccgtcctc ggtcacctcg
     6781 tcgcgcagca cgatgcgccc cgcgcgggcg gtgtagtgca ggctcttccg ccccaccttg
     6841 agggtatgcc gcgtggtcac cagatcgtcg cgcggcgcca ccggcttggt cttggcctgc
     6901 gtcgtcgtgg tgaccgtcgt ggtcacctcg ccgggtgcct cgagcgcctc cggcccatcg
     6961 ccggccaccg ccggcggggg cgcctgagcg ggggtgtcgg aaggagtgtc gtcggccatg
     7021 gagagaacct atccaccctc cgcgggctat ttgaggaact tcgaggtgcg ccggtcggcc
     7081 agcggcttgc caccggtctg gcaggtggcg cagtactgca gcgaggagtc ggcgaacgac
     7141 acctcccgca cgacatcgcc gcattccggg cagacctccc cggtgcgccc gtgcacccgc
     7201 atccccgccc gttttgcgtc cttcacttcc gcggccggct tgccggaggc cgcggagacc
     7261 gcagcggcca gggtctcgcg catcgcctca tacagccggg ccaccgcgtc cgcgtcgagc
     7321 gatccggcga tcgcgaaggg cgacatcttc gccacgtgca ggatctcgtc ggagtaggcg
     7381 ttgccgatgc cggcgatgat gccctggtcg cgcaacaccc ctttgatctg ggcccggcgc
     7441 ccgtgcagga tcgccgtgaa ctcctgctcg gtgaaaccgg gatccagcgg atccggaccg
     7501 agcgacgcga tgcccggcac ctgctccggc gagccgacca catacgccgc cagtcgcttc
     7561 ttcgtcccgg cctcggtcag gtcgaaaccc gacccgtcgt ccagacgcac ccgcagggcg
     7621 atcggcgact tgcccggccg gatcacggtg gcgggcaacg catccgacca tcgcagccag
     7681 ccggcccgag cgagatggaa aatgagatgc gttccgccgc aatccatgtc gatgaacttg
     7741 ccgaagcgcg acaccgcgtc caccggcgcg ccgaccaggg cctccggtgg cgggttgaag
     7801 gtcttcagca cggtgatcga gccgacctcc accccaacga cggccaaatc ctttgtgcgg
     7861 gaacgcaaga actcgaccag ggcctcgacc tcgggaagct cgggcatcgg tcactcctcc
     7921 acgacgacat cgctggggat gggcagccgg cccagggcga ccttgagcag ccggagcagc
     7981 ccgtagccgt cgctggcccg gcagatcggc acggtgtcga gcttgcggcg cgccgacccc
     8041 agctcgtcct gcgccagcgg ggtgaagtgc gagagcaact gctcggtcgc cgacaggcgc
     8101 cggatcgcga tagcgcgcac ccgctcgggc cgctgctcgg cgaactcgcc gtagatggcc
     8161 gggtcgtgct ggccgtcgtc cccgaccagc acccactcga tcgacggcag ttcgcgggcg
     8221 aggcgatgca ggcaggcccg cttgtggtcg cggccggagc ggaaccaccc ggtgttggtc
     8281 ggtccccagt ccgtcatcag caggggcccg gcgggaaagc ccttgtcccg caggaagcgc
     8341 gtcaggggcg gcacgacatt ccacgcgccc gtcgagacat acacgatggg cgctcccgga
     8401 tgctcctcga gcaattcgcg gtacatcgtc gccatgcccg gcaccgcggt ccgtccgcct
     8461 tcgttgacga ggaaggtgtt ccacgcggcg atcatcggcc gcggcagcat cgtgcggatg
     8521 acggtgtcgt cgatatcgct gacgatgccg aaggtgacgt cgtcggcgac gatgcgcacg
     8581 tgcgcctccg cctcctgggt ccccggggcg gtgatccgca ccggttgcca gccgggcggc
     8641 atgccgtgat cggtgaccgt gacgtccagc aggcccgagc gatccgtgcg cgtgtgcacc
     8701 gtccgctcac ccacggtgac actgaccggc acattcaccg cgggagcgct gacgaaggaa
     8761 cgccagccgc gccgcccgct gaacgcgcgc gagcggacca gctccgagac ctcgtccggc
     8821 tccgtcgcat ccggtctctt gctcagcagc agccgggcca gcacccgcgc ctgcgtcgca
     8881 ctgccgtatc cgggataggg caccacctgc gcgccccagc cgcgccgcgt cagcaggccg
     8941 gccttcgcgc ggttccagcg atcctcgacg atcgccgcgg cgtggggctt ggccatgccg
     9001 ctcagcgtaa tagaggctgg tccggtgcct ccccccgaac atcccctcgc cgcgatcctg
     9061 cgcgatgccg ccgccggccg ctggcccgcc gacgacggcg gctggagcca cctgccgggg
     9121 tggtatgccg gtgtcggcgc catcgtcgcg ctgaccggac acgccttcgt ctgctccgac
     9181 cggcagctgg accgcgaccg gctggacgca ttgggctgca acggattcgg gggcgccaca
     9241 ttgccgcagg tggtgctggc ggtcgccggt cggcgcggct gggtcgactg cctcgacgcc
     9301 ctgctcgtgg gcacgggccg cggccgcgag ccggctccgc tcgtgccgcg ccctgatctg
     9361 tcggcgcacc cacgggcggt caacgcccgc cgcagccggc acgcgacgga ggtcttgggg
     9421 tatgccgatc cccgccgccg cgatgtcgcg acggtggcgc gggggctggc tgggatgacg
     9481 gagatcgggg tcgaggcggc cgcgccaggg accgggtcgc ggctcttcgc ggatgtcctc
     9541 gacagccggc cggtcggcga ggtggtggtg gccggggtgg cgccgggcaa tgcgcgatcc
     9601 ctccggtcgg ccctggccgc cggattcgcc ataatcggat cggtccagat catgaccgtg
     9661 cccaccgcgc accgaggagc cacgtgaccg agcgccaggc cgccgagcca ctgcgcatca
     9721 acacgatggg ctgccggtgg gaggtcgaca tctcccgcct cgaggcaccc ctcgcgcagc
     9781 ggttgcgcga gttgtgggcc gcgtgcagtg actggcgccc ggaccgctcc gagccggacg
     9841 gcttcccgga agtgctgacg gcctaccacg cgggcacgtc ggatcgcccg gacggcagcg
     9901 tgatcgctct ccagcccgat gtcgagtccg ctccctacgc gttctccggg gccatcacct
     9961 tgcgctgcat cgagcgtcag gccggccgga tgacgctcct gcacagcgcc gctctcgccc
    10021 tgcccgattc gggggcgacg gtgatcctga tcgccgagtc gggcacggga aagaccacgg
    10081 ccgcccgccg gctcggcgag cggctcggct acctcagcga cgagacggcg gtgatccggg
    10141 gcgacttgac gctcatcgcg cacccgaagc cgctgtcgat cattcccgag ccggggcgcc
    10201 ccaagatcga ggtcgcgccc gaggagatcg gtctcctgcc cgccccggat cagccctggc
    10261 ttgccgcggc cgtcctgctc tcccggtcgc cggatgttgc cgcgccggtg ctggagcctg
    10321 tcgacgtttt cgaggccctc gccgaggtca ttccgcagag ttcgtccctg ccgctgcagg
    10381 agcagccgct cgacaccctc gccggcgtcc tcacggccgg cgcggggtgt ttccggttgc
    10441 gctacagcga gatcgacgcg gcggcagacc tcgtcgtcga tgtgctgcgc tccgtcagcc
    10501 ggggagagca gcggtcgcgc acgtggcagg tggtgacccc gccgcaggac cagcgcaccg
    10561 ggccgtgggt ggcctcgccg gcggaggagg gagagttgcc gcccggcggc cgcgtcgtcc
    10621 gcgctccgtg gcgcgacgcc ctggccgacg aggacggtga cgtgttcgcc ctcaacggtc
    10681 cgcgccccgt gcggctcatg gccctcggcg cgagcatctg gcgcggctgc gccgaaccgc
    10741 gcacggtgga ggagatccgc gaccagatcg tcgccgagca cggcccgcac cccgacgccg
    10801 attcccttgt gcgacaagcg attgccacgc ttgccgacga gggtttggtc cgtttcgtcc
    10861 cggcctgacg gcgggttcgg ccagcctagc gcggaggggt cggcggctcg gtggggatat
    10921 tgcgcacgag ccggcgcagc gagcgatcgc cgaccccgag ggcgacgcgg acggcgtccc
    10981 aattgtccgt atgccgttcc tcacccgtgg ccgccacggc ccgggcgaag cccgccagct
    11041 gttcggccag ggcgcggccc gtcgccgtcc ggttcggtcg tttgcgcgcg gagtagcaga
    11101 acgcccaggc gaggaggagg aggagcagag cggcggcata cagcgcaatg gcgtaggtcc
    11161 agtagccgcc gaggagcagg ttcgtggaga acaggatcat gatggccgca atggacccgg
    11221 cgaatgaggc atacaaccgc ggggtgtagc tgacgtaatc gggccggccg gtgtgccagc
    11281 gccgggaggc gacatcctgc gacagcgccc ccagcgccca gtcgcgccac tcgaaacgcg
    11341 tgttgcggcc cccgatgatc cgcagcaatc gctgttggaa gtccgaaagg ccactcgtgt
    11401 cggcgtcgcg acggaccgtg gcacggtcgt cctcgtcgag gtcgatcacc ccggcagcct
    11461 gcaggtcgag gagcgcggcg gcaatcgccc gctcggggtc gccgtcgtgc agcagcgccg
    11521 ccatctgctc cggtctcagg tcgcggggca gatcgaattg cacgacggcg tcacggaccc
    11581 gatcgttcac gcgcgcagtc ggatccgcgc cgagcggcaa cagattgcga gcagtgatcg
    11641 tcgactggtt gtcccggccg gcgcggcgcg cgtgggtgag gatcgggatc agcaggacgg
    11701 acgccgcgac gaggcagccg acggcgatgg ccagcgccca gggcaggaag tcgagcacct
    11761 cgcggctgcg cgccacctcg gtgaacggcc ggtcggccgg cagagtcacg cgtccgcctc
    11821 ctcgagcagg gtccgggagg cgatggcgac gacgaggggg atggcgacgg tggcaccgac
    11881 ggccgggatg gcgacgccgg agtcgttgat gaagaacccg atcgtcatca cgatcagcca
    11941 cgacaccagg ccggcccgca gcgtcggcac ccgctcgaaa gcgcctgcga gcgaacgcga
    12001 tccccacgac gtgggacgga gcaggacata gatgacgaag accagtccga agggcacgag
    12061 caccgtcagc gggctgctga tgaggatgcc ccagttctgc tccgccttgc ggatgacgat
    12121 gtcgagcgcg ccgccggtgc cgagcttgtc gaagaagcgg ccgaggtggc tctggcttgc
    12181 cgccggccgc agcgagtcga ggaagccgac gaggaggaag agcccggcgg tgacgagggc
    12241 gatgatcgcg ccgcggcgcc acgtcatacc caccccgagc aacgcgagga ggaaataggc
    12301 gaggccgggc aggtaggcgg gcggcccgcc gccgtcggcc ccccacgccg ggttgccgtc
    12361 gatgaccacg gtgagcagcc cgatcgccgc gacgacggcc gccgccgccc accgccccca
    12421 gcgtcgcagg gcctggtgcg ccgcggcgat cgccagcatc agcgaggccg tcgcgaagat
    12481 cgagaaggtg acattgccca tcccgtagaa ccggccgccg acgacgggct gcagacccat
    12541 gagggaggac tgctgcagcc gcgacccggt catgacgtcg atcgccagga ccgccatcgt
    12601 gatccccccg accactgcca ccggccccat cagatgccgc ccccagggcg gcaggaacgc
    12661 gagcccggca atgagcgcga cgaacagcgc gaccgcgccg acgattgccg gcaggggatg
    12721 cgaaacgcgc caccagggca ggagattcgc gaggaaggtg gccgcgggca cgctcgccgc
    12781 gagcaccccg acgatgtgca cgagccggat cgcgcgggtg cgctgggcga gggttgcgcc
    12841 ctcccgccgc cagacgcgcc acgtgaagag gtagatcgcc agctgggtga ggatcagccc
    12901 gtagaagaag atcggcacca gcgggtggat ctcgtgcgag gcgttgtcga cgtcggtcag
    12961 cgcgaggtgg cgccgctcca ccgctgccgc gtcgttcggc ctgtgctcgg tgtcgagcag
    13021 cgccccgccg agcccgagat cggtggtgtc gatgcccgcg agccgcagca gggtgacggt
    13081 caggtcggag ctttgcatca ggtcggggat gcgggtggag ggcgaggaga gctgccccgg
    13141 accgtatgcc ggcccggtca ccaccaccgg ccggagccgc gagcccatgc cggcgtcgga
    13201 gagcccggcg acgatgagcg tgtcctggtc gtccaggccg gcggcaacgg tggcgatgcg
    13261 gtcgtcgacg gcgcgcacct ggtcggccac cgaggtgcct gggctcgcct cgcccgcggc
    13321 gaggtcgccg gggtcgcgca cggcgcccgc gtcgatgagc gcgaccttcg catcgccgag
    13381 ggcggcgtgc accgagtcga gtgttgccgt ggcccagtcc accggccggt atgccgcgac
    13441 ctcgcccgag ctctgcgcca gcccgagcgc cgcgcccgga ccgatcgcga ccgccggtat
    13501 gccggcgcct gccagttggt cgccgaggag gccggggtcg gcctcgaacc gcaacgaggc
    13561 ggccgccttc tccagctccg cccagccctc gacggtggcg tgagagccgg tgaccgtcac
    13621 cgccgggagg ggcgggcacg gctctttgag gttccgcgtg gcggcgccgt cggccgtcgg
    13681 cgccgcggcc cgctgcccgg cggacaggga gagccagccg tcgaccgggc aggtgttggg
    13741 gttgaccgac cggacgctgt tggtgctcga cgacccgccg ccgagcaggt gccacagata
    13801 gggcgtcgcg tccggggaga catccgacca cgtgaacccg ccggtgcc
//
LOCUS       DAOPJG010000079        13589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4747_length_13589_cov_7.660854, whole genome shotgun sequence.
ACCESSION   DAOPJG010000079 DAOPJG010000000
VERSION     DAOPJG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 13589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13589
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4747_length_13589_cov_7.660854"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKU75_08770"
     CDS             <1..441
                     /locus_tag="PKU75_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPF81009.1"
                     /translation="ILHPMGAYLLHRGLATLTIRVERQQRTAVAVAAALAEHPAIRRV
                     MHPSRPGGDPLALVGRQMSGPGSILAFEMRDGYEAALAVAKRVHLITHAVSLGGVDTL
                     IQHPAGLTHRPVASEAKPGPGILRLSVGLEDASDIIDDLVHAIE"
     gene            complement(410..994)
                     /locus_tag="PKU75_08775"
     CDS             complement(410..994)
                     /locus_tag="PKU75_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF81010.1"
                     /translation="MTSTLGFAVLSLLARRELTGYELAQQMKRPVGYFWHAHHSQIYP
                     ELARLESAELVRHTVIDGAGPRDTKRYTITAAGMEALRAFVASDFEPQPVRDLETLKI
                     WSIWTVDPDAAVLLVEQLREVHRERLETYRRELAELTSGGTAEDPSHPDFGSWLTLEG
                     GVRNRAAALEWCDWMLTQLRRPGAVTRSRAPDRR"
     gene            1030..1887
                     /locus_tag="PKU75_08780"
     CDS             1030..1887
                     /locus_tag="PKU75_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81011.1"
                     /translation="MKRAASCGVAVVVAVSACGGQSSPQPPPATGASAVTTSGASSSP
                     RPSVSRTTPPPETRLEEECGLGAKGPIEGVRPDAESEIRYGRIGSGSTVAIFLHQSPG
                     SMCGWMDYVGLATKQAEFTALTVNSCGKGDTRCSKALSDNPVGFLTPIVEHARTELGA
                     DRVVVVGASAGGAVALAGGQQAGADAIVDLSGPAYWRGYAEAEPAAAATTVPLLIVCS
                     DSDFQMKPERLRAAVQGSPAKVKKYLDPPEGGHGWDIVTEEPGDTVKLTETGRLVLDW
                     ITGDYPASG"
     gene            1994..3187
                     /locus_tag="PKU75_08785"
     CDS             1994..3187
                     /locus_tag="PKU75_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81012.1"
                     /translation="MFGFETRRPRILFALLGFLLAWALAPGPSDAATLGASGVPRVAP
                     APVALPATTAEFVALQRGDIEGRRFIGDGGFGIRTRNGTLINFYGDTVVSATGNPQYP
                     SAATQKTYMMRNNAIIHGGGRIRSATSGTRLLSLNSFVDVPAAQRIPGGTNYYWPASA
                     ATRYDATAKVDRVYVFLSHMFTSDTGTGLWNFSQVGSRLAEYTFDAKGTLTLTRLLAT
                     PMKANDPAGIKWGAGAFVDAGYLYAFGSSKIDDPWIWGNDFYLARIPVSSIGTATAWR
                     FWTAAGWSRSATAVIPVIPNPLGLEATLAMVRDPATGAFSVSYKEFTFIGSRVMRITA
                     PALTGPWTADPEPLATLTPVVPDGYTYCGTDIPLAGSARGVIVSHGNFTPGTPLEFLG
                     IRAVI"
     gene            complement(3195..4442)
                     /locus_tag="PKU75_08790"
     CDS             complement(3195..4442)
                     /locus_tag="PKU75_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81013.1"
                     /translation="MTTARDHLIGLLLGAEEDWPTAFEALVRRMGLLTTADGIEHRIE
                     TERVTIEPFNLRDKARQDLCIDRLAYWYYHPREWLKKASLMDDVYLLNSPFTFQSMEK
                     HSAYCAMLRLGMKVPNTILVPYKNPVDNARWAYTSAKYNRSFDLDALADELGYPLYMK
                     PFDGGGWRGVSKVDDVTGLHKSYDESGEMLMHLQESIDYDVFARALTIGPETMVMKFR
                     PELPMHDRYEVSHDFLTPEAGQEAITISQTVNAFFRWEFNSCEMLVKGDQIYPIDYAN
                     ACPDVAVTSLHYYFPWAMKALAKWSAFCVATDRIAKTQVDTEPWFAVADNDQLDYDAK
                     IVAYQRLADAHFDTERYNEFCATTLSHIDEITYEYVTSPEFRSMLTSTIHQTYPTHEW
                     ERFEAHFGGLLTMWSDDNAHLAG"
     gene            complement(4481..5227)
                     /locus_tag="PKU75_08795"
     CDS             complement(4481..5227)
                     /locus_tag="PKU75_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPF81014.1"
                     /translation="MSARVELPIPGVDATLGVVRHGHFGRPMLVFPSEAGHAEDFAGN
                     GMLDAIGDLVESGRVTLFCVDSLDGWSWSASDQPTEERARRHRLYHAWLEQSLLPHIA
                     FEMGGWQSEIITFGVSMGAYHAVHFTLQRADVAPLAIGLSGNYDPSTWRGWGDIGDAT
                     YFANPMAYVRGAEGDHLNWLRSRASLLLVVGQGPFEWEPTKSYPSTLAFADVLREKGI
                     THQLDVWGHDSAHDWPWWQRQLAHHLPRFV"
     gene            complement(5285..6193)
                     /locus_tag="PKU75_08800"
     CDS             complement(5285..6193)
                     /locus_tag="PKU75_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPF81015.1"
                     /translation="MTQPPVPGSAARGAIDLTGLSDGSAPSGAPVAGGTDQIHIKATD
                     ASFPETLNRSVRVPAVLVVVSSRVPESVVYLGEVVEVATALRGRIQVVSLDIDANPGM
                     LRALQVQSVPMTLGLLQGQVLPLFAGVAAKEQLRGVLDQVLELAVQQGVTGRLELAGP
                     AAEETLPPLHQAAFDAIERGDLDAAAAAYDQALAADPKDHDAEIGRAQVALLQRTAGA
                     DPTAARAAAADNPDDVAAQTLVADLDLLGGHVEDAFGRLIDLVKRTSGEERTAARDHL
                     VQLFAVVGNQDERVKRGRTALMSALF"
     gene            complement(6243..7247)
                     /locus_tag="PKU75_08805"
     CDS             complement(6243..7247)
                     /locus_tag="PKU75_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81016.1"
                     /translation="MRSAPTSRLVAGIAGFGLAAGALTGCGFPERVVGLHDAKVETVD
                     GAGVPEATAVTITQRALAQAGAAFAAKASDTKTHKAILTGPALRIAALPDKYTDEAGT
                     SLKNPATPTILAVSAGRDWPRRILATTLVDGVQWLHVLVSEKPAEAYKLWVTVPMLPG
                     SSVPALPPLSTGTDLVEGDDGLVADPPAVLNAYGHLLDIPAPKDKKWAKLVGVKDPYA
                     TGVLAASKAQVKALGKLGTFTRQHDPETKSAESMAFRLADGSALAFGQQQRVDLMKPT
                     SKAKRLDLPSDLASLAGEKSVTKQLRLDWLLTFATVIPKDKAATVIGVSEQLRDIEAK
                     "
     gene            complement(7244..7897)
                     /locus_tag="PKU75_08810"
     CDS             complement(7244..7897)
                     /locus_tag="PKU75_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81017.1"
                     /translation="MAILKRLVGILLALAGLVPLAVGSWFAVKLGPSGEATFTVPDPG
                     TTPVVFGPEVLNRTDLPVRIDVTTKPGGTARAIVGTSADIAAVLGDAERRTMTGVHVR
                     EWTALTTRSGSGATPDLNVRDVFRINEDLPERGSITMTQATAPETLVIAPTGPGFAAL
                     SATWARDTWFYQALVLAAAGALLTTVGWLLARPGPKRRVVHVPAGTPVEATRAGVTR"
     gene            7947..9059
                     /locus_tag="PKU75_08815"
     CDS             7947..9059
                     /locus_tag="PKU75_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HPF81018.1"
                     /translation="MTHPPEITKSSRLVTDVMADDSLPRRRDESRAAKVESVATYDVM
                     WIDRVSGGAWRATREELPQLLARETGFVWVHIPDLDRDAREMLRSVFGLHPRAIEEMA
                     VRNHVARLHYHKTYLLLVVHSPVPTGSGRVESLELDQVVGTNYLITSPGPAAGSPVAA
                     ESGATAEVADRLVAGRLTPTTPIALSWAITSTITNAMERSVNDLARDVGRLEQQVVER
                     SAPRKSEVFLDELFTTRHALGRITSIAAQTGEVFGRAEYLLDGTGDPEDVRLIGDSRD
                     QFMRLAGIARAQDEFLRGVTEFYRARTDTRATVAMERLAVIVAITLPVIALASVASMN
                     VISYGRTSWPWLIGILLAMFVVSAILLRWARRQGWW"
     assembly_gap    9111..9210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9230..10195)
                     /gene="meaB"
                     /locus_tag="PKU75_08820"
     CDS             complement(9230..10195)
                     /gene="meaB"
                     /locus_tag="PKU75_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPF81019.1"
                     /translation="MTRRVDVAAVVAGVRERSPRALARALSLVESGSPDAADLLAALG
                     APSSDRRAIVVGITGAPGVGKSTTTNALVGELRRRGRRVAVLAVDPSSPFSGGALLGD
                     RIRMGEHSTDPGVFIRSMAARGHLGGLAVAAPQAIRVLERAGYDVILLETVGVGQSEV
                     EIASRADTNLVLLAPGMGDGIQAAKAGILEIADVFGVNKADREGADATVHDVRALIRL
                     AARPAGAWRPPVRKLSAASGEGVPELLDAILEHHARAVADGSLERRRRSRAAAEVEAL
                     VLGAVRDAIDDRVTAAAGEVAAGAAEPHTAARMILDDLLGARPGA"
     gene            complement(10233..11432)
                     /locus_tag="PKU75_08825"
     CDS             complement(10233..11432)
                     /locus_tag="PKU75_08825"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPF81020.1"
                     /translation="MSDRPVSVIVAGARTPMGRMSGSLKGFSGSDLGGFAIKGALEKS
                     GVKPEDVDYVIMGQVLYAGEGQIPARQAATKAGIPMSVPALSINKVCLSGLDAIALAD
                     QLIRAGEFEIVVAGGQESMTNAPHLLEKSRDGFKYGNVTLRDHMDYDALSDVFTKQGM
                     GGLTDAANVGDYAATREEQDAFSARSHQNAAKAWKDGFFDDEVVAVSIPQRKGDPVEF
                     KADEGIRADTTPDSLAGLRPAFNKEGTITAGSASQISDGACAVVVMSKAKAEELRLTW
                     LAEIGAHGNVAGPDSVLQQQPAEAIKHACAKEGIDPKDLDLIEINEAFAAVGISSTKA
                     LGVDPEIVNPNGGAIAMGHPLGMSGARVVLTLANELKRRGGGVGAAALCGGGGQGDAL
                     IVRVPSA"
     gene            11570..12004
                     /gene="mce"
                     /locus_tag="PKU75_08830"
     CDS             11570..12004
                     /gene="mce"
                     /locus_tag="PKU75_08830"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPF81021.1"
                     /translation="MTTDLFTMIDHVGVAVADLDEAIAFYSDTYGMRMLHEEVNEEQG
                     VREAMMAVGDSGSCIQLLAPLSPASTIAKFLDRSGPGIQQMAYRVADIDAVCDTLRER
                     GLRLLYDVPKRGTSNSRVNFIHPKDAGGILVELVEPAADAQH"
     gene            complement(12023..12292)
                     /locus_tag="PKU75_08835"
     CDS             complement(12023..12292)
                     /locus_tag="PKU75_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81022.1"
                     /translation="MSPYLAGHPPKQDRSLWPPILQIAMAVAILGLARWADLHASQVI
                     SVDQWAYNSLAAVLYVFGVLWTPFAIYSLIRVLRNRRRDDGPVLR"
     gene            12474..>13589
                     /gene="ccrA"
                     /locus_tag="PKU75_08840"
     CDS             12474..>13589
                     /gene="ccrA"
                     /locus_tag="PKU75_08840"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HPF81023.1"
                     /translation="MQPILDAITSGERTPEAYAALNVPESYKGATVHKDEVGMFEGLA
                     SRDKDPRKSLHIDEVPVPELAPGEALVAVMASAINYNTVWTSIFEPVPTFGFLERYGR
                     LSPYAKRHDLPYHVVGSDLAGVVLRTGPGVHLWKPGQEVVAHCLSVELENADGHNDTM
                     MDPEQRIWGFETNFGGLAELAVVKANQLMPKPAHLSWEEAASPGLVNSTAYRQLISKN
                     GAAMKLGDRVLIWGASGGLGSYATQMALAGGATPICVVSSEDKAEICRKMGAELIIDR
                     RAEGYQFWNEEGTKQNPKEWQRLGKRIRELTGGYDVDIVFEHPGRETFGASVYVTRKG
                     GTITTCASTSGYMHEYDNRYLWMNLKRIISSHFANYRE"
BASE COUNT         2121 a         4592 c         4661 g         2115 t
ORIGIN      
        1 atcctgcacc cgatgggggc gtacctgctg catcgtggcc tggctacctt gaccatccgc
       61 gtggagcgtc agcagcgcac ggccgttgcg gttgctgccg cgctggccga gcacccggcg
      121 atccggcggg tcatgcatcc gtcgcgcccg ggcggggacc cgctcgctct tgtgggccgg
      181 cagatgagtg gcccaggctc gatcctcgcg ttcgagatgc gcgacgggta tgaggcggca
      241 ctcgccgtcg cgaaacgcgt gcacctgatc acccacgccg tttcgctcgg cggcgtggac
      301 accctcatcc agcatcccgc cggcctgacg caccgtcccg tggcctccga ggccaaaccc
      361 ggcccgggca tcctgcggct gtcggtgggg ctggaggacg cgagcgacat catcgacgat
      421 ctggtgcacg cgatcgagtg acggccccgg ggcgccgcag ctgagtgagc atccagtcgc
      481 accactcgag tgcggccgca cgattgcgca cgccgccttc caatgtcagc cacgagccga
      541 agtcggggtg gctcgggtcc tcggccgtcc cgccggacgt caactccgcc agctcgcggc
      601 ggtaggtctc cagtcgctcg cgatggacct cccgtagttg ttccacgagc agtacggccg
      661 catcgggatc caccgtccag atcgaccaga ttttcagcgt ctccagatcc cgcacgggct
      721 gcggctcgaa gtccgaggcg acgaaggcac gcaacgcctc catgccggcg gcggtgatgg
      781 tgtaacgctt ggtgtctcgc ggtccggctc cgtcgatcac ggtgtgccgc accagttctg
      841 cgctttccag ccgcgccagc tcgggataga tctgtgagtg gtgggcgtgc cagaagtagc
      901 ccaccgggcg cttcatctgc tgagcgagtt catagccggt cagctcccgt cgggccagca
      961 ggctcagcac ggcgaacccg agcgtcgacg tcatggacac agcctagagg cagatgtcag
     1021 aattgacata tgaaacgcgc agcgtcctgt ggtgtcgcag tcgtggtggc cgtgtccgcg
     1081 tgcggcggcc agtcgtcgcc gcagccaccg ccggcgaccg gtgcgtccgc cgtgaccacg
     1141 tccggggcct cgtcctcgcc ccggccttcg gtcagccgga ccacgccgcc ccccgagacg
     1201 cgactcgagg aggagtgcgg cctcggcgcc aaggggccga tcgagggcgt gcgcccggac
     1261 gccgagagcg aaatccgtta cggccgaatc ggttccggat cgacggtggc gatcttcttg
     1321 caccagtcgc cagggagcat gtgcggctgg atggactatg tcggccttgc gacgaagcag
     1381 gccgagttca ccgcgctcac cgtgaactcg tgcggcaagg gcgacacccg gtgttcgaaa
     1441 gccctgtccg acaacccggt cggcttcctc accccgatcg tcgagcacgc ccggaccgag
     1501 ctgggcgccg accgcgtggt cgtcgtcggc gcctcggccg gcggggccgt cgcgctggcg
     1561 gggggccagc aggccggtgc ggacgcgatc gtcgatctgt ccggtcccgc gtactggcgc
     1621 ggctacgccg aggccgaacc ggctgctgcc gccacgaccg tgccgctcct gatcgtctgc
     1681 tccgacagcg acttccagat gaaaccagaa cggctgcgcg ccgcggtgca gggctctccc
     1741 gccaaggtca agaagtacct cgatcccccc gagggcggcc acggctggga catcgtcacc
     1801 gaggagcccg gggacacggt gaaactgacc gagaccggca ggctcgtgct cgactggatc
     1861 accggcgact atcccgccag cggctgaccg ggaccaacca gccatcacgg tgtccttcca
     1921 atgcaggact ggtggcacgt cctagccacc tgcctaatgt gtgagcgaac gatctgctcc
     1981 tcggaggcac ctcatgttcg gcttcgaaac tcgccgcccg cgaatcctgt tcgccctctt
     2041 gggctttttg ctcgcgtggg ccttggcgcc gggtccgtca gatgcggcga ccctgggcgc
     2101 ctcgggggtc ccgagagtcg cgccggcacc ggtcgccctc ccggccacga cggcggagtt
     2161 cgtggcgctg cagcgggggg acatcgaggg gcgtcgcttc atcggcgacg gcggattcgg
     2221 cattcgcact cgcaacggca ccctcatcaa cttctatgga gataccgtcg tcagcgccac
     2281 cggcaacccc caatacccca gtgccgcaac gcaaaagaca tacatgatgc gcaacaacgc
     2341 gatcatccac ggcggcgggc gcattcgcag tgccaccagc gggacacgac tgctcagcct
     2401 gaactccttc gtcgatgtgc ctgccgcaca acgaatcccg ggcggaacga actactactg
     2461 gcccgccagt gcggcaacgc gctatgacgc cacggccaag gtggatcggg tctacgtctt
     2521 cctcagccac atgttcacgt ccgacaccgg gaccggcctg tggaatttct cccaggtcgg
     2581 cagtcgtctg gcggagtaca ccttcgacgc caagggcacc ttgaccctca ccaggttgtt
     2641 ggccacgccg atgaaagcca atgaccccgc tgggatcaag tggggcgccg gtgccttcgt
     2701 cgacgcgggc tatctctacg ccttcggctc gagcaagatc gacgacccgt ggatctgggg
     2761 caacgacttc tatctcgccc ggattccggt ctcgagcatc gggacggcga cggcctggcg
     2821 cttctggacg gcggccggct ggtccaggtc ggccacagcc gtcatcccgg tcatcccgaa
     2881 tccgctcggg ttggaagcca ccctcgccat ggttcgcgac cctgccacgg gggcattctc
     2941 ggtgtcctac aaggaattca ccttcatcgg gagccgcgtg atgcgcatca cggcgcctgc
     3001 cttgaccgga ccctggacgg ccgaccccga gccgttggcc acgttgacgc ccgtggtgcc
     3061 ggacggctac acctactgtg gcacggacat acccctcgcc ggctccgccc gcggagtaat
     3121 cgtcagccac ggcaacttca cacccgggac gcccctggag ttcttgggga tccgtgccgt
     3181 gatctagccg ggactcaccc cgcgaggtgg gcgttgtcgt cggaccacat ggtcagcaga
     3241 ccgccgaagt gggcctcgaa acgctcccac tcgtgcgtcg gataggtctg gtggatcgtc
     3301 gaggtgagca tcgagcgaaa ctcgggcgac gtcacatact cgtaggtgat ctcgtcgata
     3361 tgcgacaacg tcgtcgcgca gaactcgttg tagcgctcgg tgtcgaagtg cgcgtccgca
     3421 agccgttggt aggcaacgat cttcgcgtcg tagtcgagct gatcgttgtc ggcgacggcg
     3481 aaccacggct cggtgtcgac ctgggtcttg gcgatccggt cggtcgcgac gcagaaggcc
     3541 gaccacttcg ccagcgcctt catcgcccac gggaagtagt agtgcagcga ggtgacggcg
     3601 acgtcgggac aggcgttcgc gtagtcgatc gggtagatct gatcgccctt gacgagcatc
     3661 tcgcaggagt tgaactccca gcggaagaag gcgttgacgg tttgcgagat ggtgatcgct
     3721 tcctggccgg cctcgggagt caggaagtcg tgggagacct cgtagcgatc gtgcatcggc
     3781 aactcgggac ggaacttcat caccatcgtc tcggggccga tggtcagggc gcgggcgaag
     3841 acgtcgtagt cgatggactc ctgcaggtgc atgagcatct caccggactc gtcgtaggac
     3901 ttgtgcagcc cggtgacgtc gtcgaccttg gagacaccgc gccagccgcc gccgtcgaag
     3961 ggcttcatgt agagcgggta gccgagttcg tcggcgaggg cgtcgaggtc gaaggagcgg
     4021 ttgtacttgg cggacgtgta ggcccagcgg gcgttgtcga cggggttctt gtagggcacg
     4081 aggatggtgt tgggcacctt catgccgagc cgcaacatcg cgcagtaggc cgagtgcttc
     4141 tccatcgatt ggaaggtgaa ggggctgttg agcagataga cgtcgtccat caacgacgcc
     4201 ttcttgagcc actctcgcgg gtggtagtac cagtaggcga gccggtcgat gcacaggtcc
     4261 tgacgcgcct tgtcccgcag gttgaacggc tcgatcgtca cccgttcggt ctcgatacgg
     4321 tgctcgatgc cgtctgccgt cgtgagcaga cccatgcggc gcaccaacgc ctcgaaggcc
     4381 gtcggccagt cctcctctgc gccgagcagg agaccgatca gatggtcacg ggctgtggtc
     4441 aaggcgtggc tcctttgttg ggtagggcgc tgatgcaacg tcagacgaaa cggggcaggt
     4501 ggtgggccag ctggcgttgc caccacggcc agtcgtgggc gctgtcatgg ccccagacgt
     4561 cgagttggtg cgtgattccc ttctcccgca agacatcggc gaaggcgagt gtggacgggt
     4621 aggacttggt cggctcccat tcgaagggtc cttgaccgac gacgagcagc aggctcgccc
     4681 gcgaccggag ccagttcagg tgatcgccct cggcgccgcg cacataggcc atcgggttgg
     4741 cgaagtaggt cgcgtcgccg atgtcgcccc agccccgcca ggtgctgggg tcgtagttgc
     4801 cggagagccc gatcgccaag ggcgcgacgt cggcgcgctg caaggtgaag tggaccgcgt
     4861 ggtaggcgcc catcgagacg ccgaaggtga tgatctcgct ctgccagccc cccatctcga
     4921 aggcgatgtg gggcaacagg ctctgctcca gccaggcgtg gtagagccgg tggcggcggg
     4981 cgcgttcttc ggtgggctgg tcgctggcgg accagctcca gccgtcgagg gagtccacgc
     5041 agaagagcgt cacccggccc gactcgacga gatcgccgat cgcgtcgagc atgccgttgc
     5101 cggcgaagtc ctcggcatgt cctgcctcgg acgggaagac gagcatcggc cgcccgaagt
     5161 ggccgtgacg gacgacaccg agcgtggcat cgacgccggg gatggggagc tcaacacggg
     5221 cggacatggg cgctatccaa cctcacagat cgccgggagg ctagcgcgac actcgacgcg
     5281 gtcctcagaa gagcgcgctc atcaatgcgg tgcgtccgcg cttgacccgc tcatcctgat
     5341 tgccgaccac ggcgaacagc tgcacgaggt gatcgcgggc cgcggtgcgc tcctctccgc
     5401 tggtgcgctt caccaggtcg atgaggcgac cgaaggcgtc ctcgacgtga ccgccgagca
     5461 ggtcgagatc ggcgaccagg gtctgggcgg cgacgtcgtc ggggttgtcg gccgcggcgg
     5521 cgcgggccgc ggtcggatcc gctccggccg tccgctgcag gagggcgacc tgggcgcggc
     5581 cgatctcggc gtcgtggtcc ttgggatcgg cggcgagcgc ttggtcgtat gccgcagcgg
     5641 cggcgtcgag gtcgccgcgc tcgatcgcgt cgaaggccgc ctggtgcagc ggcggcaggg
     5701 tctcctcggc agccggtccc gcaagctcga gtcggcccgt gactccttgc tgcacagcaa
     5761 gttcgagaac ctgatcgaga actccgcgca gctgctcctt cgcggccacg ccggcgaaga
     5821 gcggcaacac ctggccctgc agcagtccga gcgtcatcgg gaccgattgc acctgcagcg
     5881 cccgcagcat gccggggttg gcgtcgatgt cgaggctgac gacctggatg cgcccgcgca
     5941 acgccgtcgc gacctcgacg acctcgccga gatagaccac cgactccggc acccgggagg
     6001 agaccacgac gagcaccgcg ggcacccgca ccgagcgatt gagtgtttcc gggaacgagg
     6061 catcggtcgc tttgatgtgg atctggtcgg tgcctccggc gacgggagcc ccgctcgggg
     6121 cgctgccgtc ggacagaccg gtcagatcga tcgcgccgcg ggcggcgctt ccgggcacgg
     6181 gtggctgcgt cataccgtca tcatttcgta tgccgcggcg cagacctatt cggtgccgcg
     6241 ggctacttgg cctcgatatc gcgcaactgc tcgctgacgc cgatcaccgt ggccgccttg
     6301 tccttcggga tgaccgtggc gaaggtgagc aaccaatcga ggcgcagctg cttggtgacc
     6361 gacttctcgc ccgccagcga cgccaggtcg ctgggcaggt cgaggcgctt ggccttggag
     6421 gtcggcttca tcaggtcgac ccgctgctgc tgcccgaacg ccagtgcgct gccgtcggcc
     6481 agccggaagg ccatcgattc tgcggacttg gtctccggat cgtgctgccg ggtgaaggtg
     6541 ccgagcttgc ccagggcctt gacctgtgcc ttcgaggccg ccaggacacc ggtggcatag
     6601 gggtccttga cgccgaccag ctttgcccac ttcttgtcct tgggcgccgg gatgtcgagc
     6661 aggtgaccgt aggcgttcag cacggcgggc gggtcggcca ccaggccgtc gtcaccctcg
     6721 acgaggtcgg tgccggtcga cagcggcgga agagcgggga cgctcgaccc gggcagcatc
     6781 gggaccgtca cccacagctt gtaggcctcc gccggcttct cggacaccag gacgtggagc
     6841 cactggacgc cgtcgacgag ggtcgtcgcg agaatccggc gcggccaatc ccggccggcc
     6901 gagaccgcca ggatcgtcgg cgtcgccgga ttcttcaaag aggtgccggc ctcgtcggtg
     6961 tacttgtcgg gcagtgcggc gatccgcagg gccggcccgg tgaggatcgc cttgtgcgtc
     7021 ttggtgtcac tcgccttggc ggcgaaggcg gcaccggcct gcgccagcgc ccgctgcgtg
     7081 atcgtcaccg cggtcgcctc cggcacgccg gcgccgtcga ccgtctcgac cttggcgtcg
     7141 tggagtccga cgacccgctc gggaaagccg cagccggtca acgcgcccgc ggcgagcccg
     7201 aaaccggcga tgccggcaac caggcgcgag gtgggagcgc tcctcatcgg gtcacccccg
     7261 cacgcgtggc ttcgacggga gtgcccgcgg gcacgtgcac gacccgccgc ttggggccgg
     7321 gacgggccag caaccagccc acggtcgtca gcagggcacc ggccgcggcc agcacgagcg
     7381 cctggtagaa ccacgtgtcg cgagcccagg tggcgctgag cgcagcgaag ccggggccgg
     7441 tcggcgcgat gacgagggtc tcgggggccg tggcctgggt catggtgatc gacccccgct
     7501 cgggcaggtc ctcgttgatc cggaagacgt cgcgcacgtt gagatccggt gtcgctccgg
     7561 agccgctgcg ggtcgtcagg gcggtccact cgcgcacgtg cacgccggtc atggtgcgtc
     7621 gctcggcgtc gccgaggacg gccgcgatgt cggccgaggt gccgacgatc gccctcgcgg
     7681 tgccccccgg cttggtggtc acgtcgatgc gcaccggcag atccgtgcgg ttgaggacct
     7741 ccgggccgaa gacgacgggg gtggtgccgg ggtcgggcac ggtgaaggtc gcctcgccgg
     7801 aggggcctag tttgacggcg aaccagctgc cgacggcgag cgggaccagg ccggccaggg
     7861 ccagcaggat cccgacgagg cgtttgagaa tcgccacggt gagtcgactt tcgtttggag
     7921 aaacacgggg agcggaagac tccacgatga cccatccccc ggagataaca aaatcgtcac
     7981 ggctcgtgac agacgtcatg gcggatgatt ccctgccgcg gcggcgcgac gaatcccgcg
     8041 cggctaaggt cgagtccgtg gcgacctacg acgtcatgtg gatcgaccgg gtttcgggtg
     8101 gcgcgtggcg cgccacccgt gaagaactgc cccagttgct cgcgcgggag acgggtttcg
     8161 tctgggtcca catcccggac ctggaccgcg acgcccgcga gatgttgcgc tcggtcttcg
     8221 ggttgcaccc gcgggccatc gaggagatgg ccgtccgcaa ccacgtggcc cggttgcact
     8281 accacaagac ctacctgctg ttggtcgtgc acagtccggt cccgacgggt tcgggccggg
     8341 tcgaatccct cgaactcgac caagtggtcg gcacgaacta cctgatcacg agcccgggac
     8401 cggcagccgg cagcccggtg gccgccgaat ccggcgccac cgccgaggtt gccgaccgtc
     8461 tcgtcgccgg ccggctgacc cccacgacgc ccatcgcctt gtcgtgggcg atcacctcga
     8521 cgatcaccaa cgccatggaa cgcagcgtca acgacctggc ccgcgacgtc ggacggttgg
     8581 aacagcaggt cgtcgaacgc tccgccccgc gcaagagcga ggtcttcctc gacgagttgt
     8641 tcaccacccg ccatgccctg ggccgcatca cgtccatcgc cgcccagacc ggtgaggtct
     8701 tcgggcgggc ggagtatctc ctcgacggga ccggggatcc ggaggacgtg cgcctgatcg
     8761 gcgactcccg cgatcagttc atgcgcctgg ccggcatcgc ccgcgcccag gacgaattcc
     8821 tccgcggggt caccgagttc tatcgcgccc gcaccgacac ccgggccacc gtggcgatgg
     8881 agcggctcgc cgtcatcgtc gccatcaccc tccccgtcat cgcgctcgcc tcggtggcga
     8941 gcatgaacgt catctcctac ggccgcacca gctggccgtg gctgatcggc atcctgctcg
     9001 ccatgttcgt cgtctccgcc atcctgttgc gctgggcccg ccgccagggc tggtggtagg
     9061 agaacacccc acgaagttct ccgctcgtcc ctcgctccga tacttcgcga nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgctccgata cttcgcgaat caagccccgg
     9241 gccgagcgcc gagcaggtcg tccaggatca tgcgggcggc ggtatgcggt tcggctgcgc
     9301 ccgcggccac ctcaccggcc gccgcggtca cccggtcgtc gatggcgtcg cgcaccgcgc
     9361 cgagcaccag tgcctccacc tccgccgcag cgcgggagcg gcggcgccgc tccagtgagc
     9421 cgtcggcgac ggcccgggca tggtgttcga ggatggcgtc gaggagttcg ggaacgccct
     9481 cgccgctcgc cgcgctgagc ttgcggaccg gcgggcgcca ggcgccggcc gggcgggcgg
     9541 ccagccggat cagggcacgc acatcgtgca cggtcgcgtc ggcgccctcc cggtcggcct
     9601 tgttgacgcc gaagacatcg gcgatctcga ggatccccgc cttcgccgcc tgtatcccgt
     9661 cgcccattcc gggggcgagc agcaccagat tggtgtcggc ccgcgaggcg atctcgacct
     9721 cggactgacc gacgccgacg gtctcgagga ggatcacgtc atacccggcc cgctccagca
     9781 cccggatcgc ctggggcgca gcgacggcaa gaccccccag gtgcccgcgc gccgccatcg
     9841 aacggatgaa cacgccggga tcggtgctgt gctcccccat ccggatccgg tcgccgagca
     9901 gggcgccgcc cgagaacggc gagctgggat cgacggcgag caccgccacc cggcggccgc
     9961 ggcgccggag ttcgccgacg agcgcattgg tcgtcgtcga cttgccgacc cccggggcgc
    10021 cggtgatccc gacgacgatc gcgcggcggt ccgacgacgg cgcaccgagc gcggccagca
    10081 ggtccgccgc atccgggctc ccggactcga cgagggacag ggcccgcgcg agcgctcgcg
    10141 gggagcgctc acgcacaccg gcgaccactg ccgccacgtc gacccggcgc gtcatcgacg
    10201 gccggggacg ggcggcatac gcacctcggc gctcaggccg aggggacgcg cacgatcagg
    10261 gcgtcaccct gcccgccacc gccgcacagg gcggccgcgc cgacgccacc accgcggcgc
    10321 ttgagctcgt tggcgagggt gaggacgacg cgggcacccg acatacccag cgggtgaccc
    10381 atcgcgatgg cgccgccgtt ggggttgacg atctcggggt cgacgccgag cgccttggtc
    10441 gaggagatgc cgacggccgc gaaggcctcg ttgatctcga tcaggtcgag atccttgggg
    10501 tcgatgccct ccttggcgca tgcgtgcttg atggcctcgg ccggctgctg ctgcagcacc
    10561 gagtcgggac cggcgacgtt gccgtgcgcg ccgatctcag cgagccaggt cagcctcagt
    10621 tcctcggcct tggccttgct catcacgacg accgcgcagg cgccgtcgga gatctgggag
    10681 gccgagcccg cggtgatggt gccctccttg ttgaaggcgg ggcgcaggcc ggcgagggag
    10741 tcgggggtgg tgtcggcgcg gatgccctcg tcggccttga actcgaccgg gtcacccttg
    10801 cgctgcggga tcgagacggc gaccacctcg tcgtcgaaga agccgtcctt ccaggccttg
    10861 gcggcgttct ggtgggagcg ggcggagaag gcgtcctgct cctcacgggt ggcggcgtag
    10921 tcgccgacgt tggccgcgtc ggtcagcccg cccatgccct gcttggtgaa gacgtcggag
    10981 agggcgtcgt agtccatgtg gtcgcgcagg gtgacattgc cgtatttgaa gccgtcgcgg
    11041 ctcttctcga gcaggtgcgg ggcgttggtc atcgactcct ggccgccggc gacgacgatc
    11101 tcgaactcgc cggcgcggat gagctggtcg gccagcgcaa tggcgtcgag tccggagagg
    11161 cagaccttgt tgatggacag cgcggggacg ctcatcggga tcccggcctt ggtcgcggcc
    11221 tgacgggctg ggatctggcc ctcaccggca tacagcactt ggcccatgat gacgtagtcg
    11281 acatcctcgg gcttgacgcc ggacttctcc agggcgccct tgatggcgaa gccacccagg
    11341 tcggacccgc tgaagccttt cagcgagccg ctcatgcggc ccatcggggt gcgagccccg
    11401 gcgacgatga cggaaacggg acgatcggac atggcagcct ccaacggcga tggtgagacg
    11461 acggcttgaa gccatgctaa cgacggcccg ggcgccggac ccgggctcgt gcggtgtctc
    11521 tcaccacacc ggcggcggcg gggcgcgtgg cgtcggccaa gatggggaca tgaccacgga
    11581 tctgttcacg atgatcgacc acgtcggcgt cgcggtcgcc gacctcgacg aggccatcgc
    11641 cttctacagc gacacatacg gtatgcggat gctgcacgaa gaggtcaacg aggagcaggg
    11701 cgtgcgcgag gcgatgatgg cggtcggtga ctccggctcc tgcatccagc tgctcgcgcc
    11761 gctgtcaccg gcgagcacga tcgcgaagtt cctcgaccgt tccggcccgg ggatccagca
    11821 gatggcctac cgcgtcgccg atatcgacgc cgtgtgcgac accctgcgcg agcggggcct
    11881 gcggctgctg tatgacgtgc ccaaacgagg cacgagcaac agccgggtca acttcatcca
    11941 ccccaaggac gccgggggca tcctcgtcga actggtcgag ccggcggcgg atgcgcagca
    12001 ctgagatggc gccggccgcg gctcagcgca ggacggggcc gtcgtcgcgg cgccggttac
    12061 gcagcacccg gatcaggctg tagatcgcga acggggtcca caggaccccg aagacgtaga
    12121 gcacggcggc tagcgagttg taggcccact gatcgaccga gatgacctgg ctggcgtgca
    12181 ggtcggccca gcgggccagg ccgagaatcg ccacggccat ggcgatctgc aggatcggcg
    12241 gccacagcga ccggtcctgc ttcggcgggt ggccggcgag gtacggactc atgactccaa
    12301 ctatgtcaaa aaagacgtat cggcgatagc cgtgacggac ctcacgggtg actgcaatct
    12361 cattatccac ccgccacccc tgagccctga ctaggttcgc ctcgatggcc tgcggtgcct
    12421 cgttcgtgcc gcgcccgaat ccgccgaccg aaggagtttg gacgaccgtg accatgcagc
    12481 cgattctcga cgcgatcacc agtggcgaac gcaccccgga ggcgtatgcc gccctgaacg
    12541 tgccggagtc ctacaagggc gccacggtcc acaaggacga ggtcgggatg ttcgaggggc
    12601 tcgccagccg tgacaaggac ccgcgcaagt ccctgcacat cgacgaggtg ccggtccccg
    12661 agctggcgcc cggtgaggca ctggtcgcgg tgatggccag cgccatcaac tacaacacgg
    12721 tgtggacctc gatcttcgag ccggtgccga cgttcggctt cctcgagcgc tacggccgcc
    12781 tctcccccta tgccaagcgg cacgacctgc cctatcacgt ggtcggctcc gacctcgccg
    12841 gcgtcgtgct gcgcaccggc cccggcgtgc acttgtggaa gcccggccag gaggtcgtcg
    12901 cccactgcct ctcggtcgaa ctcgagaacg ccgacggcca caacgacacg atgatggatc
    12961 ccgagcagcg gatctggggc ttcgagacca acttcggcgg cctggccgaa ctcgccgtcg
    13021 tcaaggccaa ccagctgatg cccaagccgg cccacctgag ctgggaggag gccgcctctc
    13081 ccggcctggt caactccacg gcataccgtc agctcatctc caagaacggc gccgcgatga
    13141 aactcggcga ccgcgtgctg atctggggcg cctccggcgg cctcggctcg tatgccaccc
    13201 agatggcgct cgccggcggc gcgaccccga tctgcgtcgt ctccagcgag gacaaggccg
    13261 agatctgccg caagatgggc gccgagctga tcatcgaccg gcgggccgag ggctaccagt
    13321 tctggaacga ggagggcacc aagcagaacc ccaaggagtg gcagcgcctg ggcaagcgga
    13381 tccgcgagct gaccggcggc tacgacgtcg acatcgtctt cgagcacccc ggccgggaga
    13441 ccttcggcgc gtcggtctat gtcacccgca agggcggcac gatcacgacc tgcgcgtcca
    13501 cgtcgggcta tatgcacgaa tacgacaacc gctatctgtg gatgaacctc aagcggatca
    13561 tctccagcca cttcgccaac taccgcgag
//
LOCUS       DAOPJG010000080        13493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4792_length_13493_cov_6.280027, whole genome shotgun sequence.
ACCESSION   DAOPJG010000080 DAOPJG010000000
VERSION     DAOPJG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 13493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13493
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4792_length_13493_cov_6.280027"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5416)
                     /locus_tag="PKU75_08845"
     CDS             complement(<1..5416)
                     /locus_tag="PKU75_08845"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPF81024.1"
                     /translation="MNTKAARLIAAFVVACSALTLLVGLIVRAPGFTVRSVPLYDAGV
                     WVTNDHEGFYGRLNRAAGALDAGFFPPGSARKLDESILDVLQDAGTVVAVDRNTSTIY
                     PVDVRTGVALADQGVVIADGAVLDLRGGTMAALDPETGKVWAIRYRSDVTAGVPIQLR
                     DLAAAAKPLATLTQRGRGMKGTISDLAVSETGAVFAADVAGQTVRIEPGTGSNAFGEP
                     QLGRTATRQAVSVTLVGPHTVIADPVARTVQIGEREPELVPGEDDVVLQQPGPEDPDL
                     LVSTSRALFRLPIAGGAARSVFGAGTGAPARPVVVNGCGFAAWAGQRAATARSCGAGA
                     TTDQVVERTGSLRRPVLRVNRGQVVINDLADGKLFDVEERKATDNWVGLVRPKRAATD
                     YQGTSKDPGDTTNRKPTVERAEFGARAGRMTVLPVLDHVSDPAGRVLAVTEVSGVKGG
                     TAAIAPDLQTVRFTLNKEAKSGSFTFTVNAGGPSATGRVTVRPKSNKTNTAPTRRKSA
                     SAPLWTVPAGGVLTIPVLGSWRDAEGDAMVVESAAAPSASVQAREGALEVTAPAVPGP
                     LEITYRVTDGRKRSSAERVRIEVQDPQATTRRPATTRPDVVVTEAGRAVQINPLANDL
                     PGSDPTSAAPHLALDGDPTFTRSPKKPLTVVTDLEEGTLRVMAKVPGTYTIRYQAAYG
                     TAKPAEGEIRVEVQEAGRGRTPLAMPDTATIRGLSPVLVDVLGNDRDPAGALLTVQSA
                     VATPADSLSVAVVGGRWLRITPTGVETGAPPLVTYTVTNGKEVATGQVDVAVLPAVEE
                     DLVTVRADTASVRTGDSVLVPVLDNDSALSGSALTLLATVPDAPAAGRLRVTDPLDPK
                     LTEQDEIGAAYVSGQSVRYLAPSKVTAARTVNVEYVALTETGESASGRLTVTVNPLPT
                     GSAGNGAPTPADIEARVVQGGSVTIPVPVGEHDPDGDTTMVTGLASAPRFGRLMAFSP
                     NSLTYQAYPDFTGADSFRYAVTDAFGGVGEATVRIAIAPVGDPQLAVPIPDSITASPG
                     ATVTVSPLANDLFGAGDPVHIVPLADHNTHLPEGVGLLTETGPVTAKVPGLGESLVIK
                     YGLTGNAGPSSLSTVTVRAAKDAQIPPRITDHVAKATSVNSTTVDVLANALDPDGDSG
                     QLTVDSVGVPDATVKGGRITIPVTDAQRAVPYVVKDAAGSSAAAVVYVPAAGSGGPFA
                     KPGAGIELAVGKSRTVDLAELISSPTGDDVVLADVPEPVVTTPADAATTRASRTTLTV
                     RAGEDAGPAAVVLAVTDRARSDAATPSLVSIPVQIGDDAPQLRCPDTAFDLVAGGRPV
                     DIDIASACQVWTADPADAARLTFDATWSRDVPGVTIQGSGTDRIRLTAGTGAKPGAAG
                     EFTVAVTGEPRASAVMRVRVTDVPLPTIVPVRLDHVAPGRSVVVDIAEAMTSPLPNPV
                     YRVLSATPHNRAPVSIQTNGSRVTLTPAADASGILTVGVSATDSASADRQVHGLITLS
                     ILGTPDPPTDVTAAIVAAADSAEVSWRTGAANGSPIDHFAVEGAGRTTTCIASPCRIS
                     GLPRGEQISFVVRAHNEAGYSAASRSSAPIRIDQVPNPPSNFAATATDGSVRLTWNPP
                     AGGGSSVTGYRLTWAPGTGGGFLELGSTTSGVDIPGLTNGTTYVFSLVAFNEAGQSVP
                     VSATGTPVAPPRPIAENLREGAATTCGEPAVPCWPVLVTLSDFDGAVTCTLDSGEAYT
                     ATNGDNEFPALPYRTSLTISCSGDNGSATVSKEWGSPSSTTTTTTPPATTSTSESSTA
                     STSESSTTSTSESSTTSTSES"
     gene            complement(5547..7118)
                     /locus_tag="PKU75_08850"
     CDS             complement(5547..7118)
                     /locus_tag="PKU75_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPF81025.1"
                     /translation="MKKAAAGPPHIPDLEFIRVLSTSGGYGDVYLYREVPLERDVAVK
                     VLREGTLDAAARQRFQTEATTMARLHHPNVVAIHAVGTTRDDLAYIVMAYCPDQTLAE
                     RATGRGLSLAQVLTAGVEIGGALHSAHLAGIIHRDVKPANILTLPWGASGLTDFGVAT
                     HLVVDPDADDDDVGVSIPWSPPEMLFTATRGSVATDVYSLAATVWHLLVGRSPFELPG
                     GDNSRLAVMSRIRDSPPPPTGRADVPSSLERVLARGLAKNPAQRYASAEAFARALGVV
                     EEEARLPRSRLVVTTHQDRSPALSGPAPGEVAKTRIRALGPDRGRGPVVGATARPEPA
                     SGSTSLQHSRPLGADGPGRTVDGSSLRRRSQDLESPAPSVPQPSRAQPDRVEARQGSG
                     RRSALLVACAVGVLAAAGAYLTWHRGDEASHPAPTVSDTVTPGLGNDAGALPPGTVEI
                     EGKRDGNQLAFTWDYAGLLETDTYRWRTDDGRDGVTDEPRLELTATGRVCLEVLVVRE
                     SGASTQAYSSPRCTD"
     gene            complement(7115..8407)
                     /locus_tag="PKU75_08855"
     CDS             complement(7115..8407)
                     /locus_tag="PKU75_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPF81026.1"
                     /translation="MHNVLTASEVMHYAPGAAVAVVAPGCAVLVSGDPAGSLPTKLLT
                     ALLSDGLEAAVDIVFSSGLRNAPDFAMCGLDAQGSVRVLVRGSGAAVVENAQGRHAVD
                     TARLVADREFGAVSTVVLTLGADAGEARVPIVAGVTSADAVRCTPASAAAWPTVEDAG
                     VLTAGATGSEASSEQSSAMTDSPGESTPPAPVEFADADAPDSGAVPVENPQLRNAPIA
                     EPAEPPDGDGAAPPEGDPTPPPSLPDARLEVPWFSGERTVMAGGQVDVFADHFSPSFP
                     PPPPAPDLVGPDPDGDMDATIMRADLLTPAADPPTVGPKTAAGTIRLPDGEAFVMDRD
                     QDIVMGRAPREHPGNPAIPPHLARISDPTNEVSAQHALISRVDAVVSVTDLDSTNGTE
                     VIDGHGSRVRLAPHTPRTIEPGCVVILAGVATVAFEAP"
     gene            8583..9233
                     /locus_tag="PKU75_08860"
     CDS             8583..9233
                     /locus_tag="PKU75_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPF81027.1"
                     /translation="MVVDGIAHLVRTSGPEIRWRGAGASWPDVLELAGGPASGPRVVL
                     VEPAIGPAHETEAAIRELTQRGVAVVVHTADLRPVPIRRAVTAGALGVVLKTHSRDAV
                     LAVVRAAAAGTFTTSSEWARRLVSDARMTPQLSPREEEVLRLLAIGLPRKVIGRHMSP
                     PVATTTVVTYLNRMCEKYREIGRTISCPADVLRLAAEDGYLDLGGNYPRGSLEAAS"
     gene            9346..11634
                     /locus_tag="PKU75_08865"
     CDS             9346..11634
                     /locus_tag="PKU75_08865"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HPF81028.1"
                     /translation="MKVARGVALMASLLLALVTPAAAAGGPDVTVTGRLSYVVLDPGL
                     GPDADRSAPAQARTRASLTIAGTTVPVASAVADGLAAGEVTVRVTASATGRTTPSAIA
                     ADLRSGAARIDQLAPAAGQRSEPPVLGQHHVTVVPLYWSAPPATTNALVGTTMTAVDR
                     YFNTQTHGKIRFELTRILPAEKVTLSPEDIEYCATSQLEDRARKLANLPKDQYHHVVV
                     LMQYNPNCFFAGMASIGQDAFGGEMVVINDTPSQVVWAHEYGHNLGLIHNAGRVCWSD
                     RAHQHAVPLSNDCQDVTYEDPFDLMGHGWWGWAGISSAHQEKLGVLPAGDRLALSSGG
                     TVTLNSMSTGSGLRSVYLEVGGALWDVEYHVAAGQESWIDDETYTGYDGVERTSPGAG
                     VVVRRISATADLYEEWAVVNPHIEGDGSRFERHPVLTAGESLAVPGGLLTITVKATTS
                     TTATVTLTTRADGVTRWAGADRYETAANIARLAFPGVREIYAASGLLFTDALSGAPVA
                     GMRGKPMFLMMPDQIPNRAFMELIRRDPTSVTLLGGPATLSEDLRIQLDSEFGAVSRI
                     AGEDRYATSAAISRKGFTPGVSVAYVASGLVFPDALSGAPVAARDRGPVLLTDDGTLP
                     APVAAELTRLQPASVVVLGGPASIGESVLEEIEQAAGVTPERVSGADRYAVSAEISRR
                     AFPSGADLVFVASGEKFTDALAGAPAAGAKKAPMLLVKEKAIPAVVAGELARLNPKEI
                     IVLGGDATISPAVEMALGDYVD"
     gene            11700..12374
                     /locus_tag="PKU75_08870"
     CDS             11700..12374
                     /locus_tag="PKU75_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPF81029.1"
                     /translation="MGKTEETREKLVVAALASFRERGYDATTMRLIAAEAGVSQGNAY
                     YYFEGKEALVQELYVRIQAEHRAAVAGRLRSGASLAHNLATVLHTGLDVMAPYHSFGT
                     TLLQSALRTTSPVSPFAPDSQAAREAAIWLMREAVDRSRPRIGGRLGEQLPRLLWLAY
                     LGVTLHWVIDGSPGQQRTRELVDRVSPLLGKAIRLAGTPVAKPFVTDLTALVDAMTAP
                     GRAGTP"
     gene            12371..13300
                     /locus_tag="PKU75_08875"
     CDS             12371..13300
                     /locus_tag="PKU75_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPF81030.1"
                     /translation="MSVVVIAGASGFIGRHLSAAFAAEGWQVRTIGRGVGADARWGSD
                     LRPALDGSLAVVNLAGRSVSCRYTKKTADEIFSSRTETTAALGSAIADTANPPAVWLN
                     SSTGTIYREARDRPQDERTGELGTGFSVAVARAWEHELYAAPAPVRKLALRTAITLGA
                     GGGALSPLINLARLGFGGRQGDGAQVVSWIHVDDVYAAIRHLIDAPDISGPVNLASPH
                     PVPNAELMSLVRKEFGWLAGEEARGIPLPRAALGIGGRIIRTEPELVLKSRWLAPTVL
                     QESGFRFRYPELADALAAIGRSTPRGLLPVQLG"
BASE COUNT         1964 a         4601 c         4855 g         2073 t
ORIGIN      
        1 agctctcgga ggtggaggtg gtggagctct cggaggtgga ggtggtggag ctctcagagg
       61 tggaggcggt ggagctctcg gaggtggagg tggtcgcggg tggagtggtg gtggtcgtgg
      121 tggacgacgg cgagccccac tccttcgaga cggtggcgga cccgttgtcg ccggagcagg
      181 agatggtcag cgaggtccgg tacggcagcg ccgggaactc gttgtcgccg ttcgttgcgg
      241 tatacgcctc gccgctgtcc agggtgcagg tgacggcgcc gtcgaagtca ctcaaggtga
      301 ccagtaccgg ccagcacggc acagccggct cgccacacgt ggtcgctgcg ccctcgcgga
      361 ggttctcggc gatcggccgc ggcggggcga ccggagtccc cgttgccgag acgggcacgc
      421 tctgcccggc ttcgttgaag gcgacgagag agaagacata ggtcgtccca ttggtcaagc
      481 ccgggatgtc gaccccactc gtggtggatc ccagctccag gaacccgccc cccgtgccag
      541 gcgcccaggt gagccggtag ccggtcacgg aggacccgcc gccggctggc ggattccagg
      601 tcagccgaac agagccgtcc gtggccgtgg ccgcgaagtt gctgggcggg ttcggcacct
      661 gatcgatcct gatcggggcg gacgaacgcg acgccgcgct gtagcccgcc tcgttgtggg
      721 cccggaccac gaagctgatc tgttcgcccc gggggagccc ggagatccgg cacggcgacg
      781 cgatgcacgt cgtggtcctg ccggcgccct cgacggcgaa atggtcgatg ggtgagccgt
      841 tggcggcgcc ggtgcgccat gacacctcgg ccgagtccgc ggcggcgacg attgccgctg
      901 tgacatccgt cggtgggtcg ggcgtgccga ggatggacaa cgtgatcagg ccatggacct
      961 ggcgatctgc cgaggcggag tccgtcgccg agaccccgac cgtgaggatg ccggaggcgt
     1021 ctgcagccgg tgtcagcgtg acccgggaac cgttggtctg gatcgagacc ggagcccggt
     1081 tgtgtggtgt cgccgaaagc acccggtaaa ccgggttcgg aagcggagac gtcatcgcct
     1141 cggcgatgtc caccacgacg gaccgcccgg gagccacatg atccagacgg acgggcacga
     1201 tcgtcggcag cggcacatcg gtcacccgca cgcgcatgac tgccgaggcg cgcggttcgc
     1261 cggtgacggc gacggtgaac tcgcccgccg caccgggctt cgctcctgtg ccggccgtga
     1321 gtcgtatgcg gtccgtgccg gaaccctgga tcgtcacgcc cggcacatcc cggctccacg
     1381 tcgcgtcgaa ggtcagccgc gctgcgtcgg ccggatcggc ggtccagacc tggcaggcgc
     1441 tcgcgatgtc gatgtcgacc ggacgcccgc ccgccacgag atcaaaggcg gtatccgggc
     1501 agcgcaactg cggcgcgtcg tcgccgatct ggacgggaat gctcacgagg gaaggcgtcg
     1561 cagcatccga tcgagctcgg tcagtgaccg cgagcaccac ggcagccggt ccggcatcct
     1621 cgccggccct cacggtgagg gtcgtgcgag aggcccgcgt ggtggctgcg tctgcaggcg
     1681 tggtcaccac gggctcgggg acgtcggcga gcaccacatc gtcaccggtc ggggagctga
     1741 tgagctctgc gaggtcgacc gtccggctct tacccaccgc gagctcgatg ccggcgccgg
     1801 gcttggcgaa ggggccgccc gagcctgcgg ctgggacata cacgactgcg gcggcgctgg
     1861 agccggcggc gtccttgacg acatacggca ccgcacgctg cgcgtcggtg acggggatgg
     1921 tgatccggcc gcccttgacc gtggcatcgg gcacgccgac cgagtcgact gtcaactgtc
     1981 cggaatcgcc gtccgggtcc agggcattcg ccaagacgtc gacggtcgtc gagttcaccg
     2041 aggtggcttt cgccacatga tcggtgatgc gtggcgggat ctgtgcgtcc ttcgcggcgc
     2101 ggaccgtcac cgtgctcaga gacgaggggc cggcgttacc ggtcaagccg tacttgatga
     2161 cgagcgattc gccgagtccg ggcaccttcg ccgtcactgg cccggtctcg gtcaacaggc
     2221 cgacgccctc ggggaggtga gtgttgtggt cggcgagggg gacgatgtgc accggatcgc
     2281 cagctccgaa cagatcgttg gcgagcgggc tgacggtcac ggttgccccc ggggaggcgg
     2341 tgatcgagtc agggatgggc accgcgagtt gcggatcgcc cacaggcgct atcgcgattc
     2401 ggaccgtggc ctcgccgacc ccgccgaacg cgtccgtcac ggcataccgg aagctgtccg
     2461 ccccggtgaa gtccggatag gcctggtagg tgaggctatt ggggctgaac gccatcaggc
     2521 ggccgaaccg cggtgccgag gcgaggccgg tcaccatggt cgtgtcgccg tccgggtcgt
     2581 gctcaccgac gggcacgggg atggtcacgc tgccgccctg gaccacccgg gcctcgatgt
     2641 cggccggcgt cggggcgccg ttgccggcgc tgccggtcgg gagggggttg acggtcaccg
     2701 tcaagcgccc ggatgcgctc tcgccggtct cggtgagagc gacgtattcg acgttcaccg
     2761 ttctggccgc ggtgaccttc gacggcgcca gatagcgcac ggactggccg gagacgtatg
     2821 ccgcgccgat ttcgtcctgc tccgtcagct tcggatccag cggatcggtg acccgcaacc
     2881 ggcccgcggc cggcgcgtcg ggaacggtgg ccagcaaagt cagcgccgag ccggagaggg
     2941 cggagtcgtt gtcgaggacc ggcaccagga cggagtcgcc agtgcgcacc gacgcggtgt
     3001 cggcccgcac ggtgacgagg tcctcctcga cggccggcag caccgcgaca tcgacctgac
     3061 ccgtggccac ctccttgccg ttggtgacgg tgtaggtcac gagcggaggg gcacccgttt
     3121 ccactcccgt cggtgtgatc cgcagccacc ggccgccgac gacggcgacc gacagggaat
     3181 ccgcgggagt cgcgaccgcg gattgcacgg tcaagagcgc gccggcgggg tctcggtcat
     3241 tgcccaacac gtccacgagc accggcgaca gcccgcggat cgttgcggtg tccggcatcg
     3301 ccagcggcgt gcgccccctt cccgcctcct ggacctcgac ccggatctca ccttcggctg
     3361 gcttcgcggt cccgtatgcc gcctgataac ggatggtgta ggtcccgggc accttcgcca
     3421 tgacgcgaag ggttccctct tccagatccg tgacgaccgt gagcggcttc ttcggcgagc
     3481 gggtgaacgt cgggtcgcca tcgagcgcca gatgcggggc tgcactggtg ggatccgagc
     3541 cgggaaggtc gttcgccaag gggttgatct gcaccgcccg gccagcttcg gtgacgacga
     3601 cgtcgggacg ggtggtggcc ggacgccggg tggtggcctg gggatcttgc acctctatcc
     3661 gcacccgctc cgcggaactg cgtttccggc cgtccgtgac gcggtaggtg atctccagcg
     3721 gtcccggtac ggcgggagcg gtcacttcga gtgcgccctc cctggcctgc accgaggccg
     3781 aaggtgcagc ggcggattcg accaccatcg cgtcgccctc cgcatcgcgc caggaaccca
     3841 gcacgggaat ggtcaagaca ccacccgccg gcaccgtcca caagggagcg gatgcgctct
     3901 tgcgtcgtgt cggcgcggta ttcgtcttgt tcgatttggg gcggacggtc acgcggccgg
     3961 tggcggaggg gccccctgcg ttcaccgtga acgtgaacga tccggacttg gcttccttgt
     4021 tcaaggtgaa gcgcaccgtc tgcagatcgg gcgcgatcgc ggctgttcca cccttgaccc
     4081 ccgagacctc cgtcacggcg aggactcgac ccgcggggtc ggagacatgg tcgagcactg
     4141 ggagcacggt catccggccg gcccgggcgc cgaattcggc ccgctcgacc gtgggtttcc
     4201 ggttcgtggt gtcgcccgga tccttgctgg tgccctggta gtcggttgct gcccgtttcg
     4261 ggcggaccag tccgacccag ttgtctgtcg ccttgcgctc ctcgacgtcg aagagcttgc
     4321 cgtcggcgag gtcgttgatg accacctgac cccgattgac ccgtaacacc gggcgccgca
     4381 gcgacccggt gcgctcgacg acctggtctg tcgtcgcgcc tgctccgcag ctgcgggcgg
     4441 tggctgcccg ttggccggcc caggcagcga aaccgcaccc attcacgacg acgggccgag
     4501 ccggtgcccc ggtgcccgca ccgaagacgg agcgggccgc cccgcccgcg atcggaagac
     4561 gaaagagcgc ccggctggtg gataccagca ggtcagggtc ttccgggccg ggctgttgca
     4621 gaacgacgtc gtcctctccc ggcaccagtt cgggctcgcg ttcgccgatc tgtacggtcc
     4681 gtgcgacggg gtcggcgatg acggtatggg ggccgaccag cgtgaccgaa acggcctggc
     4741 gggttgcggt cctgcccagt tgcggttcgc cgaaggcgtt gctccccgtg cctggttcga
     4801 tgcgaaccgt ctgaccggcc acgtcggcgg caaacacggc accggtttcg gacacggcga
     4861 gatcggagat cgtccctttc atccccctcc cgcgctgggt gagagtcgcc aacggcttcg
     4921 cggcggcggc gaggtcgcgc agctggatgg ggacgcccgc cgtgacgtcg ctgcggtagc
     4981 ggatcgccca gactttgccg gtctcgggat ccagggcggc catggtgccc ccacgcagat
     5041 cgaggacggc accgtcggcg atcacgacac cctggtcggc cagggcgaca ccggtgcgca
     5101 cgtcgacggg atagatcgtt gacgtgttgc gatcgaccgc gaccacggtc ccagcgtcct
     5161 ggaggacatc gagaatggac tcgtccagtt ttcgcgcgct gcccggtgga aagaagccgg
     5221 cgtccaacgc tccggccgcc cggttcaagc gtccgtagaa gccttcatgg tcgttggtca
     5281 cccagacacc ggcatcgtag agagggacgc ttcgcacggt gaagcccggt gcgcgcacga
     5341 tcagccccac gaggagcgtg agggccgaac aggcaacgac gaaggccgcg atcaggcgcg
     5401 ccgccttggt gttcactgtg tcctttccct cctgttccgg ccgcccgggt gccacgaccc
     5461 atctcggagt atccacccaa atcgcctcgt cgtcgtgtcc gctagatagg ggacccgccg
     5521 cgactcgctg accgacggaa gccgggctag tcggtacacc tcggcgacga gtaggcctgg
     5581 gtggaggcgc ccgattcccg gacgacgagc acttcgaggc agacgcggcc cgtcgcggtt
     5641 agttccagcc gtggctcgtc ggtcacgccg tcgcggccgt catcggttcg ccagcggtag
     5701 gtgtcggtct cgaggaggcc cgcgtaatcc catgtgaacg ccagctggtt gccgtcgcgt
     5761 ttgccttcga tctcgaccgt gcccgggggc agtgcgccgg catcgttgcc gagacccggc
     5821 gtcacagtgt cggagacggt cggcgccggg tggctggcct cgtcacctcg gtgccacgtc
     5881 aggtaggcgc cagcggcggc aaggacgccc acggcgcagg caaccaatag ggcggagcgc
     5941 cggccggagc cctgccgcgc ctcgacgcgg tccggctgcg ccctagacgg ctgcgggacc
     6001 gagggggctg gagactccag gtcctggctg cggcgacgca gggacgagcc gtcgaccgtg
     6061 cgcccaggcc cgtcggctcc gaggggccgg gagtgctgaa gggacgtcga tccggacgcc
     6121 ggttcgggtc gcgcggtcgc cccgaccacg ggcccgcgcc cacgatccgg ccccagggcg
     6181 cggatccgtg tcttggcgac ctcgccgggc gccgggcccg aaagggcagg cgagcgatcc
     6241 tggtgtgttg tgacgacgag ccgggagcgg ggcagacggg cctcctcctc cacgacgccg
     6301 agcgcgcgcg cgaaggcctc ggcgctggca taccgctgcg ccgggttctt ggccaggccg
     6361 cgcgccaaga cgcgctcgag ggaactcggg acgtccgccc gcccggttgg tgggggcggc
     6421 gagtcgcgga tccggctcat taccgccagc cgggagttgt ctccacccgg gagttcgaag
     6481 ggcgacctgc cgacaaggag atgccacacg gttgcggcga gggagtagac atcggtcgca
     6541 acggagcctc tggtggctgt gaagagcatt tctggcggcg accacggaat cgagacgccg
     6601 acgtcgtcgt catccgcgtc cgggtcgacg accaggtgcg tcgcgacgcc gaaatcggtc
     6661 aacccggaag ccccccacgg cagagtgagg atattggccg gcttgacgtc tcgatggatg
     6721 atgcccgcca ggtgcgcgct gtgcagagcc ccgccgattt cgaccccagc cgtcaggacc
     6781 tgtgcgaggc tcaacccgcg gccggtggcc cgctcggcga gggtttggtc ggggcagtac
     6841 gccatcacga tgtaggcaag gtcgtcccgc gtggtgccga cggcgtggat cgccacgacg
     6901 ttcggatggt gcaggcgggc catcgtcgtc gcctcggtct ggaagcgttg gcgcgcggcg
     6961 gcgtcgagtg tcccctcgcg cagcactttc acggcgacgt cgcgctccaa cggcacttcg
     7021 cggtagaggt acacgtcgcc gtaacccccg ctggtgctca gaacacgaat gaattccagg
     7081 tcggggatgt gcggggggcc ggcagccgcc ttcttcacgg cgcctcgaac gcaaccgtcg
     7141 cgacgcccgc gagaatgacg acgcagcccg gctcgatcgt tctgggggtg tgcggcgcca
     7201 gccgcacgcg ggaaccatgt ccgtcgatca cttcagtgcc attggtcgag tccaggtcgg
     7261 tgaccgagac gactgcatcg acgcgcgaga tcagcgcgtg ctgcgcggag acttcattgg
     7321 tcggatccga gatgcgggcc aggtgcggcg gtatggccgg gttgcccggg tgttctcgtg
     7381 gcgcgcggcc catcacgatg tcctggtccc gatccatcac gaaggcttcg ccgtcgggca
     7441 ggcggatcgt gcctgccgcg gttttcgggc ccacggtagg cgggtcggcg gcgggtgtga
     7501 ggaggtccgc gcgcatgatc gtggcatcca tgtcgccgtc cgggtcgggt ccgacgagat
     7561 ccggagcagg cgggggtggg ggaaagctcg gtgagaagtg gtcggcgaag acgtccacct
     7621 gcccgccggc catgacagtg cgttcccccg agaaccacgg gacttcgagc cgggcgtccg
     7681 gtagcgacgg cggtggcgtg gggtcgccct ccggcggggc agctccatcc ccgtccggag
     7741 gttcggctgg ttcggcaatc ggcgcattgc gaagctgcgg attctcgacc ggtacggcac
     7801 ctgagtcggg ggcgtccgcg tctgcgaact cgaccggtgc cggcggtgtg ctctcgcccg
     7861 gcgaatccgt catagccgag gattgctcac tcgaggcttc ggaaccggtg gcgccggctg
     7921 tgaggacgcc tgcatcctcg acggtgggcc aggcagcagc gctcgcggga gtgcatctga
     7981 cggcatcggc cgaggtgacg ccagctacga tcggcacccg cgcctcaccg gcatccgcac
     8041 ccagcgtcag aacgacggtg gataccgcac cgaactcccg gtctgcgacg agccgtgccg
     8101 tgtcgaccgc gtgtcggcct tgggcattct cgaccacggc agcgccgctg ccgcggacga
     8161 gcacccgaac ggacccctgt gcgtccagcc cgcacatcgc gaaatcgggg gcattgcgca
     8221 accctgacga gaagacgatg tcgacagcag cctcgagccc atcggacaag agggcggtca
     8281 gcagcttcgt gggcaaggag cctgcggggt cgcccgacac gagcacggcg catccgggcg
     8341 cgacgacggc gaccgccgcc ccaggtgcat agtgcatgac ctccgacgcc gtcagtacat
     8401 tgtgcacggt ttgacgatat gcccgcgtcg aggattttgg gaatgctggc cgctcctgga
     8461 cggtgccggc ctggtgaggg cgggtgtatc aggggcgtcc aacccgcctc tgcactggag
     8521 acgtcccccc gaacacgtgc ggagcaggac atgatcaccc tcgtcgtcgg cgacatccac
     8581 cgatggtggt ggacgggatc gcccatctcg tgcgcacctc gggaccggag atccggtggc
     8641 gcggcgcggg tgcctcgtgg ccggacgtgc tcgagctggc cggcggaccg gcctcggggc
     8701 cacgcgtcgt gctggtcgag ccggcgatcg ggccggcgca cgagacggag gcggccatcc
     8761 gggagctgac gcagcgcggc gtggcggtcg tcgtgcacac cgccgacctg cggccggtgc
     8821 ccatccgccg ggcggtcacg gccggcgcgt tgggcgtggt gctcaagacc cactcgcgcg
     8881 acgccgtgct ggcggtcgtc cgcgcggccg cggccgggac cttcaccacc tcgagcgagt
     8941 gggcgcgccg gctggtgtcc gatgcgcgga tgacaccgca actcagcccg cgcgaggagg
     9001 aagtgctgcg cctgctggcg atcgggttac cgcgcaaggt gatcggccgg cacatgagcc
     9061 cgcccgtggc gacgaccacg gtcgtgacct atctcaaccg catgtgcgag aagtatcgcg
     9121 agatcggccg gacgatctcg tgtcccgccg acgtgctgcg cttggcggcc gaggacggct
     9181 atctcgacct gggcggcaac tacccccgcg ggtccctcga ggccgcgagc tgaccggtga
     9241 cagatatcgt cacaaccatc ggggcttgtg cggcgtcttc cttggtgacg ccgacgctga
     9301 ccaggagacg ccatgaacca ccgccatcgc cttcccactg cccgcatgaa agtggcccgt
     9361 ggggtggccc tcatggcctc actgctcctc gccctggtca ccccggcggc cgcggcgggc
     9421 gggccggacg tgaccgtcac cggccggctg tcgtatgtcg tcctcgaccc cgggctgggc
     9481 ccggacgccg accgcagcgc accggcgcag gcccggaccc gggcgtcgct gacgatcgcc
     9541 gggaccaccg tgcccgtcgc gtcggcggtg gccgacggac tggcggccgg tgaggtcacc
     9601 gtccgggtga cggcgagcgc cacgggacgg acgaccccgt cggccatcgc ggcggacctg
     9661 cgctccggcg cggcccgcat cgaccagctc gcgcccgccg ccgggcagcg ttcggagccg
     9721 ccggtgctcg gtcagcacca tgtcaccgtc gtgccgctct attggagtgc gcccccggca
     9781 accaccaacg ccttggtggg caccaccatg acggcggtcg acaggtactt caacacccag
     9841 acccacggca agatccgctt cgagctcacc aggatcctcc cggcggagaa ggtcacgctg
     9901 agcccggagg acatcgagta ctgcgccacg agccagctcg aggaccgggc gcgaaaactg
     9961 gcgaacctgc ccaaggacca gtaccaccac gtcgtggtcc tcatgcagta caaccccaac
    10021 tgcttcttcg ccggtatggc atcgatcggt caggacgcgt tcggcggcga gatggtcgtc
    10081 atcaacgaca ctccttcgca ggtcgtctgg gcccatgagt acggtcacaa cctcggcctg
    10141 atccacaacg ccggccgggt gtgttggtcc gatcgcgcgc accagcacgc cgtcccgctg
    10201 tcgaacgact gccaagacgt cacctatgag gatcccttcg acctgatggg gcacgggtgg
    10261 tggggctggg ccggcatcag ctccgcgcac caggagaagc tcggcgtcct gcccgccggc
    10321 gaccggttgg ccctgtcctc gggcggcacg gtcaccctca actcgatgag caccggctcc
    10381 ggcctgcgca gcgtctatct ggaggtgggc ggcgccttgt gggacgtcga ataccacgtt
    10441 gcagccgggc aggagagctg gatcgacgac gagacctaca cgggatacga cggagtcgaa
    10501 cgcacctcgc ctggtgccgg ggtcgtggtg cgccgcatct ccgcgacggc cgacctgtac
    10561 gaggaatggg ccgtcgtcaa tccgcacatc gaaggtgacg gcagccgctt cgaacgccac
    10621 cccgtgctga cggctgggga gagcctggcc gtgcccggcg gcctgctcac gatcacggtc
    10681 aaggccacca cgtccacgac ggcgacggtc acgctcacca cgcgggccga cggggtgacg
    10741 cggtgggccg gggcagaccg ctacgagacg gccgccaata tcgcccggct ggccttcccg
    10801 ggcgtccggg agatctacgc cgcatccggc ctgctgttca ccgacgccct cagcggcgcg
    10861 ccggtggccg gcatgcgggg gaagccgatg ttcctgatga tgcccgacca gatcccgaac
    10921 cgggccttca tggagttgat ccgccgggac ccgacgtcgg tgaccctgct cggcgggccg
    10981 gcgacgctgt cggaggacct gcggatccag ctcgacagcg agttcggcgc cgtttcccgg
    11041 atcgccggcg aggaccggta tgccacctcg gccgcgatct cccgcaaggg gttcaccccc
    11101 ggagtctcgg tcgcgtatgt cgcgtcgggg ctcgtcttcc ccgacgccct ctcgggggcg
    11161 ccggttgcgg cgcgggatcg gggcccggtg ttgctgaccg acgacggcac tctgccggcc
    11221 ccggtcgcgg ccgaactgac tcggctgcaa ccggcttcgg tcgtcgtcct cggggggccg
    11281 gcttcgatcg gggagtccgt cctggaggaa atcgagcagg cagccggcgt cacgccggag
    11341 cgcgtgtccg gagccgaccg gtatgccgtg tccgccgaga tctcccggcg cgccttcccc
    11401 tcgggtgccg acctcgtctt cgtcgcctcc ggtgagaagt tcaccgacgc cctggcgggc
    11461 gccccggctg cgggggccaa gaaggcgccg atgctgctgg tcaaggagaa ggcgatcccc
    11521 gcggtcgtgg ccggcgaact cgcccggctg aaccccaagg agatcatcgt cctcggcggc
    11581 gacgccacca tcagccccgc cgtcgagatg gccctcgggg attacgtcga ctgacgcagg
    11641 taggtacggg tccgacaccc gtctttttga acacgttcaa aaagtggtag cgtcacgcca
    11701 tgggcaagac ggaggagacg cgggagaagc tggtggtggc tgccttggcc tcgttccgcg
    11761 agcggggtta cgacgccacg acgatgcgcc tgatcgctgc ggaagccggt gtgtcgcaag
    11821 gcaatgccta ctactacttc gagggcaagg aggccttggt ccaggagctg tatgtccgca
    11881 tccaggccga gcaccgggcc gccgtggccg ggcgactgcg cagcggggca tcgctcgcgc
    11941 acaacctggc gacggtgctg cataccggcc tcgacgtcat ggcgccctat cactccttcg
    12001 gcacgaccct cctgcagtcc gcactgagga ccacctctcc ggtcagcccc ttcgcacccg
    12061 actcccaggc ggcgcgcgag gcggcgatct ggctgatgcg cgaggccgtc gaccgcagcc
    12121 ggccgcgcat cggcggacga ctcggggagc agctgccacg gctgctctgg ctcgcctacc
    12181 tcggcgtcac gcttcactgg gtcatcgacg gcagtccggg gcagcagcgc actcgggagc
    12241 tcgtcgaccg ggtcagtccg ttgctgggca aggcgatccg gctcgccggg acacccgtcg
    12301 ccaagccctt cgtcaccgac ctcaccgccc tcgtcgacgc catgacggcg ccaggacggg
    12361 ccggaacgcc atgagcgtcg ttgtcatcgc cggggcctcg ggcttcattg gtcgccacct
    12421 cagtgcggcc ttcgcggccg aggggtggca ggtgcgcacc atcggccggg gtgtcggcgc
    12481 cgatgcccga tggggcagcg acctgcgtcc ggcgctcgac gggtcgctgg ccgtcgtcaa
    12541 cctggccggg cggtcggtca gctgccgcta caccaagaag accgcggacg agatcttcag
    12601 ctcccgcacc gagaccaccg cggcgctcgg cagcgccatc gccgacaccg cgaatccccc
    12661 tgcggtgtgg ctcaattcga gcaccggcac catctaccgg gaggcccgcg accggcccca
    12721 ggacgaacgc accggcgaac tcggcaccgg gttctccgtc gccgtcgccc gggcctggga
    12781 gcacgagctg tatgccgcgc ccgccccggt ccgcaagctc gccttgcgca ccgccatcac
    12841 gctcggcgcc ggtggcggtg ccctcagtcc gctgatcaac ctggcgcgcc tgggtttcgg
    12901 cggccggcag ggagacggcg cacaggtggt ctcgtggatc cacgtcgacg atgtgtatgc
    12961 cgccatccgc cacctcatcg acgccccgga catcagcggg ccggtcaatc tcgccagtcc
    13021 gcacccggtt cccaacgccg agctgatgtc gttggtgcgc aaagagttcg gctggctagc
    13081 gggggaggag gcacgcggca taccgttgcc gcgcgcagcg ctcggcatcg gcggacggat
    13141 catccggacc gagcccgaac tcgttctcaa gagtcgctgg ctggcgccga cggtgctgca
    13201 agagagcggt tttcgcttcc gctatccgga gttggccgat gccctcgccg cgatcggccg
    13261 gtcgacgccc cgagggctgc tgcccgtcca gctcggctga tcccctcccc cgaacgacgg
    13321 cgtccgcggg gacggtcgcg atatcgaccg tccccgcgga cgccggtctt gcagggtgct
    13381 ggtcttgcgg gggtgttggt cttgcggggg tgttgggctt gcgggggtgt tgggcttgca
    13441 ggggctgggc tcagccccag ttgtccttgg cccggtcgcc ggcgtcctcg aca
//
LOCUS       DAOPJG010000081        13287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4897_length_13287_cov_7.190296, whole genome shotgun sequence.
ACCESSION   DAOPJG010000081 DAOPJG010000000
VERSION     DAOPJG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4897_length_13287_cov_7.190296"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /gene="sigE"
                     /locus_tag="PKU75_08880"
     CDS             <1..561
                     /gene="sigE"
                     /locus_tag="PKU75_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HPF81031.1"
                     /translation="WEQIVEEHSARVYRLAYRLTGNVHDAEDLTHDVFIRVFRSLGSY
                     TPGNFEGWLHRITTNVFLDKMRRKQRIRFDALSDEASARIPSRELSPEQAYADRHFDD
                     DVQRALDALPPDFRAAVVLCDIEGLSYEEIAATLGIKLGTVRSRIHRGRALLREALSH
                     RAPTPQPVAAGGRTLATGRLAPAGVS"
     gene            558..1187
                     /locus_tag="PKU75_08885"
     CDS             558..1187
                     /locus_tag="PKU75_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPF81032.1"
                     /translation="MISFGPAPAMRGCLGERVSDYVDGTLGAGEQLACDRHLTACQGC
                     RAEIELERRLRATLRTEPAVPDALMAMLMAVSQEIPVQAPLSRPARTGVSRPAPAGGS
                     VPSVPLRGPGMPPALPIAPVPVLAPSAPAQHRSALRAAAFATAAAGASVAAVWAFSLA
                     PSAGVRPAPASVLPRPAAEPSIHPSAPATSPIVVRARAFSSPDLAQSTP"
     gene            1670..2989
                     /locus_tag="PKU75_08890"
     CDS             1670..2989
                     /locus_tag="PKU75_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPF81033.1"
                     /translation="MTAPIPTAYPPATTYPPATTYPPATAPLPQAEPTTQATLPSPGG
                     HAAYPPAVGGPTPWAGGSPAGWSTPPPAGPAGATAYAPGRGRGPGWASVLGIAGVTGL
                     IAALAGAGLGASLAGDGLFDRGTGTSVERPAGSFAALAAAALPSVVTIRATGSGSGAT
                     GSGFVYDTAGHVITNNHVVEGVGEEITVVLNDGSTVEAEIVGTDPAYDIAVLDTKRTD
                     LKPLPMADSKGVVVGDEVLAMGAPLGLESTVTSGIVSAVDRPVVAGDSTSRSYINAIQ
                     TDAAINPGNSGGPLIDTTGKVIGVNSAIATPPGSSSGNAGNIGLGFAIPSAQVVKTAD
                     QLIRTGSSEHPIIGVLLDESYSGPGAKVKADNGGDDPITAGGPADKAGIQPGDVIVRF
                     DGHEVSGPDDLIVAIRAKSVGDTVAMTVRRDGQPVDVTMTLQAAPKK"
     gene            3028..3459
                     /locus_tag="PKU75_08895"
     CDS             3028..3459
                     /locus_tag="PKU75_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPF81034.1"
                     /translation="MMGWDELAIVIVLAIVIIGPTRLPEYAAKLARGIRQMRVLADGA
                     KAQLREQMGPEFDDVNWRQYDPRQYDPRKIVRDALREPIETAWNPVRDEIEGVRSSAG
                     QIGRSSADGADAEPMEAVAAGATAVGADELARITPYDADAT"
     gene            complement(3619..4752)
                     /locus_tag="PKU75_08900"
     CDS             complement(3619..4752)
                     /locus_tag="PKU75_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPF81035.1"
                     /translation="MPQPTHDQLTAALAKVDDPEIRKPITELGMVQDLAIDDAGVASV
                     TILLTVAACPLKDTLTRDVTAALRGVPGVSDARVTLGVMTDEQRAELKTKLRGGAPEK
                     EIPFAKPGSLTRVYAVASGKGGVGKSSVTANLAAALAEQGLKVGVVDADIYGFSIPRM
                     LGVSGQPTQVDDMILPPVAHDVKVISIGMFVPGNQPVVWRGPMLHRALNQFLADVFWG
                     DLDVLLLDLPPGTGDVAISIAQLIPNAEILVVTTPQQAAAEVAERAGAIAAQTRQRVV
                     GVVENMSWLELPDGTRQELFGSGGGAQVAASLTRLLGAEAPLLGQIPIDVTLREGADA
                     GMPVVLGSPTSPAAAALRGIAKTLATRSRGLAGRSLGLTPSGR"
     gene            complement(4950..5582)
                     /locus_tag="PKU75_08905"
     CDS             complement(4950..5582)
                     /locus_tag="PKU75_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HPF81036.1"
                     /translation="MAERRPRLDQPQEQHSPWIRRPKGMNREAFGVLSEKFARFMGTP
                     QFIIWMTVFVAAWLIWNTWGPERLQFDPRDLNYTLLTLILSLQASYAAPLILLAQNRQ
                     ADRDRVALEQDRSRDERNLADTEFLTREVASLRLAMRDAATRDFLRSELRDLLEDLVS
                     EVDQRETKAARKAKRKAKAKAKAKAAAKAQARQAAARAATKPGDMPSAPQ"
     gene            complement(5575..6861)
                     /locus_tag="PKU75_08910"
     CDS             complement(5575..6861)
                     /locus_tag="PKU75_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPF81037.1"
                     /translation="MSAPVRVFVARLAGLEVFDPLGDRVGRVRDVVITFSQTRRPRVL
                     GMVVEVAGKRRVFVPMTRVTSVDGAQVITTGLVNMRRFEQRQNETLVLAELLERQVTA
                     RTDKGLLDATVEDIGIEQDRARDWSVTKVFCREGKPQGGLARLGRRRSATFVVPVEDV
                     TGLHVESEQQPAARLLEAYEDLKAADLAEAISDLSPKRRLEVAAALDDERLADVIEEL
                     PEDAQVEIMTGLETERAADILEAMQPDDAADLLSELPEEQQEQLLMLMEPDEAEDLRR
                     LLAYDDNTAGGLMTTEPVILGPEASIAEALAVVRREELPTALATTVFVTRGPHETPTG
                     RYLGMVHIQRLLREPPHASIGGILDKDVDPLEPDLLLGEVARRLANYNMIALPVVDEG
                     AHLIGAVTVDDVLDHILPEHWREDRHELTQSEVSHG"
     gene            complement(6943..7533)
                     /locus_tag="PKU75_08915"
     CDS             complement(6943..7533)
                     /locus_tag="PKU75_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81038.1"
                     /translation="MTDPASPAEAQASAMPGAAPAADPGPAPVNLTALLAEALGKSDV
                     CWLRTPGFNRAVWHAYDRDAVLIVTGPGEQQIPPLPDEVELILRSKDAGSRLLTITAR
                     VVVLPPEHAMWPVAAAALAAERLNATDDQIERWRTSGTIYVLHPYGLPVERPGAYAAE
                     AAAYPIPAATGTTGGLKPRHVGKHFAKRKPDAHSAR"
     gene            7587..8669
                     /locus_tag="PKU75_08920"
     CDS             7587..8669
                     /locus_tag="PKU75_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPF81039.1"
                     /translation="MRSAKDFFAPLAVGAPAPVREVPARPSRMIHFFDPSNEKMAAKI
                     PSMVGTVDVLLGNLEDAVKAENKEKARLGLVEIAKATDFGDATQLWTRVNSLDSPWVL
                     DDLTTLVTEIGDKLDVIMVPKVQGPEDINYVDRLLAQLEARAGLTRPILVHAILETAR
                     GMANVEEICGASPRMQGISLGPADLAADRRMKTTRVGGGHPGYLVRQDPPAGVEDAIN
                     ADRAVFQQDLWHYTIARMVDACAMHGIFPYYGPFGDIKDVVACEDQFRNAFLLGCVGA
                     WSLHPAQIAIAKKVFSPSAADVAHARRVKAAMGDGTGAVMLDGKMEDDASLKQCLVIL
                     ALADQLSAGDPELAELYKEQASEEAK"
     gene            8669..9532
                     /locus_tag="PKU75_08925"
     CDS             8669..9532
                     /locus_tag="PKU75_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPF81040.1"
                     /translation="MTTAYRPRRSVLYMPSSNERALEKAKALGCDALILDLEDAVAPD
                     AKPAARDNACAAAKSGEYGRREITIRVNGIGTEWHEADLAAASAAGPDGIVVPKVNSP
                     KEVRQLAEAMRDFGAPETTKLWAMIETPEAIFEADKIARADERLACFVIGTNDLVKEL
                     QALHVPGRAPVLTSLSMALLAARRAGIAIVDGVYNDVKDAEGFAAECRQGREMGFDGK
                     TLIHPGQIDACNAAFSPSESEIEEAEGIVAAWRDGAGTGVVTYNGKMIERLHVDIAER
                     VLAVHEAVTAG"
     gene            9651..9917
                     /locus_tag="PKU75_08930"
     CDS             9651..9917
                     /locus_tag="PKU75_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HPF81041.1"
                     /translation="MASNDTGPAESPADPGAPVTGTYAVDGMTCGHCAHAVTQELSAV
                     AGVRDVQVDLVAGGTSTVTVVSDAPLDEAAVTAALEEAGDYTLV"
     gene            complement(9972..10805)
                     /locus_tag="PKU75_08935"
     CDS             complement(9972..10805)
                     /locus_tag="PKU75_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HPF81042.1"
                     /translation="MTSDPSAFATDSFPDAPPEGERWSTWDGATHGPKPRPDWVITDL
                     GAVEADLGVLKTGKEADVHVVRRWVPDGTAEPERDVLMAAKRFRSGDHRLFHRDAGYL
                     EGRRVRRSREMRAMARRTEFGKEVISGQWAGAEFEALKALWALELPVPYPVQLTGTEM
                     LMEFVGSDGVAAPRLAATRVETRSLPGLFEQAREVLTRLALHGWAHGDLSAYNILLDG
                     DRLVFIDWPQIVDIIGNPQGFDFLHRDVHNLASWFTSKGYAVDEEHLFGDLVAAATSR
                     W"
     assembly_gap    10813..10912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11013..11828)
                     /locus_tag="PKU75_08940"
     CDS             complement(11013..11828)
                     /locus_tag="PKU75_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPF81043.1"
                     /translation="MSSGGRPVARRTLLLGAAAAATASCDLSISTSGSATTHAHSHRM
                     DGTLTSVHLAPEESAWSLLMPMGKGSRALVIGLHGKGSNGPEMMDMLALDEHVARTEL
                     AVATISGGDTYWHKRRSGVDAGAMVMQDFLPLMRSYAKIPESTPLGFLGFSMGGYGSL
                     LLASAMKPATVLGVVAQSAALWTDPGDSAEGAFDDREDFVAHDVFRRAKALSRIPIRL
                     DCGTDDPFIAANRAFARDVVPHAVTNFDAGGHTGDYWRSHAGADLDWLAAQLS"
     gene            complement(11833..13065)
                     /locus_tag="PKU75_08945"
     CDS             complement(11833..13065)
                     /locus_tag="PKU75_08945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81044.1"
                     /translation="MRRFFAPYRVLRVMPSAPHFFASAYVARLGTSMFGVAIVVMIAT
                     RYDSYALAGLVSFVGLISMAMGGPLVARAIDRFGQRRIALPAGLVVGCCLLALTVEAY
                     AGVPGWVLLVTALGQAVAPNPSTLVRTRWTHLLRERPADLHVATSFEQAAEESCFVLG
                     PALGAALASWLFPEAGVLVATTLFLVGLLGLLAHRVSEPPVHVSDEKHHVLGAFTTPG
                     LLGLAVTLTLTGAVFGGMDVVVLAYAEDNGVKALGGALVGFFAAGSLVGGLLYGLVPA
                     DGRIARRMTVWTFAMFALLAPVLWVPNIPVLAVNGFVAGLAIAPTLITAMTLCPRLVP
                     TSQLNEGMTIVVTGLLIGVGVGSAAAGQVIESYGAHRAFLVPVAAAALAFVLALLGRS
                     RLVAAERRALGSPVPVAR"
BASE COUNT         1973 a         4570 c         4784 g         1860 t
ORIGIN      
        1 tgggagcaga tcgtcgagga gcactcggcg cgtgtgtacc ggctggccta ccgcttgacc
       61 ggcaatgtcc acgacgccga ggacctcacg catgacgtct tcatccgcgt cttccgctcg
      121 ctggggtcct acacgcccgg caacttcgag ggctggctgc accggatcac gaccaatgtc
      181 ttcctcgaca agatgcgccg caagcagcgg atccgcttcg atgcgctctc ggacgaggcc
      241 tcggcccgca tcccgagccg cgagctgtcg cccgagcagg cgtatgcgga ccgccacttc
      301 gacgatgacg ttcagcgggc gctggatgcg ctgccgccgg acttccgcgc cgcggtcgtg
      361 ctgtgcgaca tcgaaggcct gagctatgag gagatcgccg cgacgctcgg gatcaagctc
      421 ggcaccgtgc gctcccgtat ccaccggggc cgggccctgt tgcgcgaggc actgagccac
      481 cgcgccccga ccccccagcc cgtcgctgcc gggggccgca cgctggcgac tgggcgcctc
      541 gccccggcag gcgtgtcgtg atctcgttcg gcccggcccc cgcgatgcgc ggctgcctcg
      601 gcgagcgggt cagcgactat gtcgacggca cgctgggcgc gggcgagcaa ctcgcgtgcg
      661 accggcacct gaccgcgtgc caaggctgcc gcgccgagat cgaactcgag cgccggctgc
      721 gggccacgct gcgcaccgag cccgccgtgc ccgatgcgct gatggccatg ctgatggccg
      781 tgtcgcagga gatcccggtc caggcccccc tctcgcggcc tgcgcgaact ggggtgtcgc
      841 gccccgcgcc ggcaggtggg tcggtgccgt cggttccgct gcgcgggccg ggtatgccgc
      901 ccgccctccc catcgcgccg gtcccggtcc tcgcgccgtc ggcgcccgct cagcaccgct
      961 cggcgctgcg ggctgccgcg ttcgcgaccg ctgctgccgg ggcctcggtc gcggcggtgt
     1021 gggcgttctc gctcgcgccg agcgccggcg tgcgtcccgc gcccgcctcg gtgctgccgc
     1081 gcccggccgc cgagccctcg atccacccga gcgctcccgc gacgagcccg atcgtggtgc
     1141 gcgcgcgtgc attctcctcg cctgacttgg cacaatcgac gccatgacga caccgggcga
     1201 gggctaccct ccgcgcgagg accagcccac gcccgctccc tgggacacct ggcactggtc
     1261 gcccgcaccg ggtcaggccg ccgttcacga ctcgcgctac gcgatgccgg ctgattcagg
     1321 gtcggcgccg ctgaccgcag gcccgcagcc cctggaggcc gatccgctcg agaccggctc
     1381 gctcgaggcc gatccgatgg aggccggtgc ggtagcgccg ccgctgggcg ccgccgcagc
     1441 ggcgcccagc gcggccgcgg cccttggctc accaccggtc tcaccccctg tctcatttcc
     1501 aggctcgacc tctggctcag ctcctgtctc gtcacctggc tcggcaccga tcagctggct
     1561 gggcggtccg gtggcgacgg ggccggcgcc cgagggggcg ggccggccgg ctggttcgac
     1621 cgcgcccgcg gcgcgtccgg cgggttctgc ccacgccggc ggcgacgcgg tgaccgcgcc
     1681 catccccacg gcatacccac cggccacgac atacccaccg gccacgacat acccaccggc
     1741 gacggcaccc ctgccgcagg ccgagccgac cacgcaggcg acgcttccct ccccgggcgg
     1801 gcacgcggca taccccccgg cggtgggcgg accgacgccc tgggccgggg ggtcgcccgc
     1861 cgggtggagc actccgccgc cggccggacc ggcgggggcc acggcatacg caccggggcg
     1921 cggacgcggc ccgggatggg cgagcgtgtt ggggatcgcc ggcgtcaccg gcctgatcgc
     1981 cgcgctcgcg ggggcggggc tgggggccag tcttgccggc gacgggctct tcgaccgggg
     2041 cacgggcacg tccgtcgagc ggccggcggg gagtttcgct gcccttgccg ccgccgccct
     2101 gccgagcgtc gtgaccatca gggccaccgg atccggttcc ggtgcaacgg gttccgggtt
     2161 cgtctacgac acagccggcc acgtgatcac caataatcac gtcgtcgagg gcgtcggcga
     2221 ggagatcacg gtcgtcctga acgacggcag cacggtggaa gccgagatcg tcggcaccga
     2281 cccggcatac gacatcgccg tcctcgacac caagcgcacg gacctcaagc cgttgccgat
     2341 ggccgactcc aagggcgtcg tcgtcggtga cgaagtgctc gccatgggtg cgccgctcgg
     2401 gctcgaatcg acggtcacgt cggggatcgt ctccgcggtc gaccggccgg tcgtcgcggg
     2461 cgactcgacg tcgcgctcgt atatcaacgc gatccagacc gacgccgcca tcaaccccgg
     2521 caacagtggc gggcccctca tcgacaccac gggcaaggtg atcggcgtca actccgcgat
     2581 cgccacgccg ccgggctcct cgagcggcaa cgccggcaat atcgggctcg ggttcgccat
     2641 tcccagcgcc caggtcgtca agaccgccga tcaactcatc cggactggca gttcggagca
     2701 cccgatcatc ggcgtgctgc tcgatgagtc ctattcgggg ccgggcgcga aggtcaaggc
     2761 cgacaacggt ggcgatgacc cgatcacggc tggtggtccg gccgacaagg cgggcatcca
     2821 acccggcgac gtcatcgtgc gcttcgacgg ccacgaggtc tccggacccg atgacctgat
     2881 cgtggcgatc cgggccaaga gcgtgggcga taccgtcgcg atgacggtcc ggcgcgacgg
     2941 ccaacccgtc gatgtcacaa tgaccctgca ggctgctccc aagaagtagc ccgctgccgc
     3001 acccggaacg caaaggacat ctgacagatg atgggctggg acgagctcgc gatcgtcatc
     3061 gtgctcgcga tcgtcatcat cggcccgacc cggctgccgg agtatgccgc caagctcgcc
     3121 cgcggcatcc ggcagatgcg cgtgctggcg gacggggcga aggcgcagct cagggagcag
     3181 atgggcccgg agttcgacga cgtcaactgg cgtcagtacg acccgcggca gtacgacccg
     3241 cgaaagatcg tccgggacgc cctgcgggag ccgatcgaga ccgcctggaa tccggtgcgc
     3301 gacgagatcg agggcgtgcg ctcctcggcc ggtcagatcg ggcggtcatc cgccgatggt
     3361 gccgacgcgg aaccgatgga ggccgtggcg gccggcgcca ccgctgtcgg cgccgacgag
     3421 ctcgctcgca tcacgccata cgacgccgac gccacctgac gccaccgcgt cgcgattgcg
     3481 tcgggcgaac gcgtcgaggc gaacccggcg tcggcataca caccaacagt cgagtgagcg
     3541 cgacgcgccc atgttcagtg cgttcaatgg gcgtgtcgcg ctcactcgac tggcgttcgt
     3601 cctgagggcg cgcaggtttc agcggccgga cggggtgagc ccgagggagc ggccggcgag
     3661 gccgcgggag cgggtggcca gggtcttggc gatgccgcgt agcgcggccg cggcgggcga
     3721 tgtgggggag ccgaggacga ccggcatacc cgcgtcggcg ccctcccgca gggtgacatc
     3781 gatcgggatc tggccgagca gcggcgcctc ggcaccgagc aggcgcgtca gcgacgcggc
     3841 gacctgcgcg ccgccgccgc ttccgaagag ttcctgacgc gtgccgtcgg gcagctccag
     3901 ccacgacatg ttctccacga ccccgacgac ccgctggcgg gtctgagcgg cgatggcgcc
     3961 ggcgcgttcg gcgacctcgg cggcggcctg ctggggggtg gtgacgacga ggatctcggc
     4021 gttggggatc agctgggcga tggagatcgc gacatctccg gtgcccgggg gcaggtcgag
     4081 cagcagcacg tcgaggtcgc cccagaagac gtccgcgagg aactggttga gcgcccggtg
     4141 caacatcggc ccacgccaga cgaccggctg gttgccgggg acgaacatgc cgatcgagat
     4201 gaccttcacg tcgtgcgcga ccggcggcag gatcatgtcg tcgacctggg tcggctgacc
     4261 cgacactccg agcatccggg ggatcgagaa gccgtagata tcggcgtcga cgacccccac
     4321 cttcagaccc tgttcggcca gcgccgcagc gagattcgcg gtgaccgacg acttgccgac
     4381 gccgcccttg ccggaggcca cggcatacac gcgggtcagc gaaccgggct tggcgaacgg
     4441 gatctccttc tccggcgctc ccccgcgcag cttcgtcttc agctcggcgc gctgctcgtc
     4501 ggtcatcacc ccgagcgtca cccgggcgtc gctcacgccg ggcacgccgc gcagcgcggc
     4561 ggtgacatcg cgggtgagcg tgtccttgag gggacaggcg gcgacggtga gcagaatcgt
     4621 cacgctcgcg acgccggcgt cgtcgatggc gaggtcctgc accatgccga gttcggtgat
     4681 gggcttgcgg atctcggggt cgtcgacctt cgcgagggcg gcggtgagct ggtcgtgtgt
     4741 gggctgcggc accccgcaac tgtatgccgc ccgctcgcct cctcccgaat ccgcggtgcg
     4801 gacgctgccg ccgcccgcag cggggctggg tttcgcgacg ggggcctggc tataagtcgg
     4861 cccgcctcgg ccaacctggc cccgcttctg tcaaacctgg ccccgctcgg cgccgccggc
     4921 agcgggccgg cgagccattg ccggggggcc tactgcggcg cggacggcat gtcgcccggc
     4981 ttggtcgccg cgcgggcggc ggcttggcgg gcctgggcct tggcagcggc cttcgccttg
     5041 gctttggcct tcgccttgcg cttggccttg cgggcggcct tcgtctcgcg ctggtcgacc
     5101 tcggagacga gatcctccag gaggtcgcgc aactcgctgc gcaggaagtc gcgggtggcg
     5161 gcatcccgca tggccagtcg caatgacgcg acctcgcggg tcaagaactc ggtgtccgcc
     5221 aggttgcgct cgtcacggga gcgatcctgt tccagcgcaa cgcgatcgcg gtcggcttgc
     5281 cggttctggg cgagcaggat cagaggcgcg gcatacgacg cctggaggct caggatcaag
     5341 gtgaggaggg tgtagttcag gtcgcgcggg tcgaactgca gccgttccgg gccccaggtg
     5401 ttccagatca gccaggcggc gacgaagacc gtcatccaga tgatgaactg cggggtcccc
     5461 atgaaccggg cgaacttctc cgagagcacc ccgaaggcct cgcggttcat gcccttgggg
     5521 cggcggatcc acggcgagtg ctgctcctgc ggctggtcga gccggggccg gcgctcagcc
     5581 atggctcacc tccgactggg tcagctcgtg gcggtcctcg cgccagtgtt ccgggaggat
     5641 gtggtcgagc acgtcgtcga cggtgaccgc gccgatcagg tgggcgccct cgtcgacgac
     5701 gggcagagcg atcatgttgt agttcgccag ccggcgggcg acctcgccga gcagcaggtc
     5761 cggctcgagt gggtcgacgt ccttgtcgag gatgccgccg atcgacgcgt gcgggggttc
     5821 gcgcaggagc cgctggatgt gcaccattcc gagatagcgg ccggtcgggg tctcgtgcgg
     5881 cccgcgggtg acgaagaccg tcgtcgcgag ggccgtgggc aactcctccc gccggacgac
     5941 ggcgagcgcc tcggcgatgg aggcctccgg gccgaggatc accggttcgg tcgtcatcag
     6001 accgccggcg gtgttgtcgt cgtaggcgag cagccggcgc aggtcttcgg cctcgtcggg
     6061 ctccatcagc atcagcagct gttcctgctg ctcctcgggg agttcggaca acaggtcggc
     6121 ggcgtcgtcg ggctgcatgg cctcgaggat gtcggcggct cgctcggtct cgagaccggt
     6181 catgatctcg acctgggcgt cctcgggcag ctcctcgatg acgtcggcga gtcgttcgtc
     6241 gtcgagggcg gcggcgacct cgaggcgccg tttgggggac aggtcgctga tggcctcggc
     6301 caggtcggcg gccttgaggt cctcgtacgc ctcgagcagc cgggcggccg gctgctgctc
     6361 actctcgacg tggaggcccg tgacatcctc gaccggcacg acgaaggtgg cgctgcggcg
     6421 ccggccgagc cgggccaggc cgccctgggg cttgccttcg cggcagaaga ccttggtgac
     6481 gctccagtcg cgggcccggt cctgctcgat gccgatgtcc tcgacggtgg cgtcgaggag
     6541 acccttgtcg gttctcgccg tgacctgccg ctcgagcagc tcggcgagga cgagtgtctc
     6601 gttctggcgc tgctcgaacc ggcgcatgtt gaccagcccg gtcgtgatca cctgggcgcc
     6661 gtccaccgac gtcacccggg tcatcgggac gaagacacga cgtttgccgg ccacctcgac
     6721 gaccatgccg aggacgcgcg ggcggcgggt ctgggagaag gtgatgacga catcgcgcac
     6781 gcggcccacc cggtcgccga gcgggtcgaa cacctcgagc ccggcgagcc gggcgacgaa
     6841 gacgcgaacc ggtgcactca caagcgccaa ggctatcggc agtgcgcggc agggcccggg
     6901 aggttctggt ggcgtgtgct cacgctcgcc gggtgtgcgc gtctagcgcg cggagtgcgc
     6961 gtcgggcttg cgcttggcga agtgcttgcc gacgtggcgg ggtttgaggc cgccggtggt
     7021 gccggttgcc gccgggatcg ggtatgccgc ggcctcggcg gcatacgccc caggccgttc
     7081 gacgggtagg ccgtaggggt gcaggacgta gatggtcccg ctcgtccgcc accgctcgat
     7141 ctggtcgtcg gtggcgttga gccgctctgc ggcgagggcg gcggcggcga ccggccacat
     7201 ggcgtgctcg ggcggcagga cgacgacgcg ggcggtgatc gtcagcagcc ggctgccggc
     7261 gtccttgctg cgcaggatca gctcgacctc gtcgggcagc ggcgggatct gctgctcacc
     7321 cgggccggtg acgatcagga cggcatcacg gtcatacgca tgccacaccg cccggttgaa
     7381 cccgggggtc cgcagccagc acacatcgct cttgcccaac gcctcggcga gcagcgcggt
     7441 gaggttcacg ggggcggggc cggggtcggc ggcgggagcg gctcccggca tcgcggacgc
     7501 ctgcgcctcg gccggggatg cggggtcggt cacatcgcca ctgtcgcaca taggcgcggc
     7561 ggcggcgcgc tgctagcgtc ccaaatatgc gtagcgccaa ggacttcttc gcccctcttg
     7621 ccgtcggcgc cccggcgccc gtccgggagg tcccggcccg gccgagccgg atgatccact
     7681 tcttcgaccc gagcaacgag aagatggccg ccaagatccc gtcgatggtc gggaccgtcg
     7741 atgtgctgct cggcaacctc gaggacgccg tcaaggcgga gaacaaggag aaggcccggc
     7801 tcgggctcgt cgagatcgcc aaagcaaccg acttcggcga cgccacccag ctgtggacgc
     7861 gggtcaacag cctggactcg ccgtgggtgc tcgatgatct gacgactctc gtgaccgaga
     7921 tcggcgacaa gctcgacgtc atcatggtgc ccaaggtgca agggccggag gacatcaatt
     7981 acgtcgaccg gctgctggcg cagttggagg cgcgcgccgg gctgacgcgg ccgatcctcg
     8041 tgcacgcgat cctggagacg gcacggggga tggcgaatgt cgaggagatc tgcggcgcct
     8101 cgccgcggat gcagggcatc tccctcggcc cggccgatct ggccgccgac cgccggatga
     8161 agaccacgcg cgtcggcgga ggacaccccg gctatctcgt gcggcaggat ccgcccgctg
     8221 gggtcgagga cgcgatcaac gccgatcgcg ctgtcttcca acaagatctg tggcactaca
     8281 cgatcgcgcg gatggtcgat gcgtgtgcga tgcacgggat cttcccctac tacggaccgt
     8341 tcggcgacat caaggatgtc gtcgcctgcg aggaccagtt ccgcaacgcc ttcctgctcg
     8401 ggtgcgtcgg tgcctggtcg ctgcacccgg cgcagatcgc gattgccaag aaggtcttct
     8461 cgccctcggc ggcggacgtc gcgcatgccc gccgggtcaa ggcggcgatg ggcgacggca
     8521 cgggcgcggt gatgctcgac ggcaagatgg aggacgacgc ctccctcaag cagtgcctcg
     8581 tcatcctcgc cctcgccgac cagttgtcgg cgggcgaccc ggagctcgct gagctctaca
     8641 aggagcaggc gagcgaggag gcgaagtgat gacgacggca taccgtcccc ggcgctcagt
     8701 cctctatatg ccgagcagca acgagcgcgc gctggagaaa gcgaaggccc tcggctgcga
     8761 cgcgctgatc ctcgacctcg aggacgccgt cgcacctgac gcgaagccgg ccgcccgcga
     8821 caacgcatgt gccgcagcga aatccggcga gtacggccgg cgtgagatca cgatccgcgt
     8881 caacggcatc ggcaccgagt ggcacgaagc cgacctcgcg gcggcgagcg cggccgggcc
     8941 tgacggcatc gtggtgccaa aggtcaactc gcccaaggaa gttcgccaat tggccgaggc
     9001 gatgcgcgac ttcggcgcgc ccgagaccac caagctgtgg gccatgatcg agacgccgga
     9061 agcgatcttc gaggccgaca agatcgctcg ggcggacgaa cgcctggcct gcttcgtcat
     9121 cggcaccaac gacctcgtca aggaactgca ggccctgcac gtgcccggtc gagcccccgt
     9181 gctgacctcg ctgtccatgg cgctgctcgc ggcccggcgg gccggcatcg cgatcgtcga
     9241 cggcgtctac aacgacgtca aggacgcgga gggcttcgct gccgagtgcc gccaggggcg
     9301 ggagatgggc ttcgacggca agacgctcat ccatcccggc cagatcgacg cctgtaatgc
     9361 cgccttctcg cccagcgaga gcgagatcga ggaggcggag ggcatcgtcg ccgcctggcg
     9421 ggacggcgcc ggcaccggcg tggtcaccta caacggcaag atgatcgagc gcctccacgt
     9481 cgacatcgcc gagcgcgtcc tggcggtgca cgaagctgtc accgcaggct gaccccactg
     9541 cggattaccc cccaggggta tgctgtggtg aggccccgcg ggaaccgcgc gggcggcatg
     9601 cgaggagtag cgaaatgacc cagcccagcg acgtcagccg caacctgccg atggcgagca
     9661 acgacaccgg ccccgccgag agcccggccg accccggggc accggtgacg ggcacctatg
     9721 ccgtcgacgg catgacctgc ggccactgcg cgcacgccgt gacccaggag ctgtccgccg
     9781 ttgccggcgt ccgcgatgtc caggtcgacc tcgtcgcggg cggcacctcc acggtcacgg
     9841 tcgtgtccga cgcgcccctc gacgaggcag ctgtgacggc ggccctcgag gaggccggcg
     9901 actacacgct cgtgtaaccg atctggctgt ctcccgaccc aactgagcag aggttccttg
     9961 gggggccagg ctcaccaccg cgacgtggcg gcggcgacga ggtcaccgaa gaggtgctcc
    10021 tcgtccacgg catacccctt gctcgtgaac cacgaggcga ggttgtggac gtcgcggtgc
    10081 aggaagtcga acccctgcgg gttgccgatg atgtcgacga tctgcggcca gtcgatgaag
    10141 acgaggcggt cgccgtcgag caggatgttg tatgccgaca ggtccccgtg cgcccacccg
    10201 tgaagcgcga ggcgggtcag cacctcgcgt gcctgctcga acagacctgg cagagaacgg
    10261 gtttcgacgc gcgtcgcggc cagccggggg gcggcgacgc cgtcggagcc gacgaactcc
    10321 atgagcatct cggtgccggt cagctggacc ggatagggca ccggcaactc cagcgcccac
    10381 agggccttga gcgcctcgaa ctcggcgccg gcccactggc ccgagatcac ctctttgccg
    10441 aactcggtgc ggcgggccat ggcgcgcatc tctcgggacc ggcgtacgcg gcgaccctcg
    10501 agataaccgg cgtcccggtg gaagagccgg tggtcgccgc tgcggaaccg cttggcggcc
    10561 atgaggacat cacgttccgg ctcggccgtg ccgtcgggca cccagcggcg cacgacgtgg
    10621 acatcggcct ccttgccggt cttgagcacg ccgagatccg cctcgacggc tccgagatcg
    10681 gtgatgaccc agtcgggtcg gggtttgggg ccgtgggtgg cgccgtccca ggtggaccag
    10741 cgctcgccct cgggtggggc gtcagggaag gaatcggtcg cgaatgcgga cggatccgaa
    10801 gtcatgggtg aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcggtggt
    10921 tcctgagcgc cctactcgta gctggtgtgc accaaccggg ccgtggcgag cgccgtgtgc
    10981 actcgcccgg atggggcggc ggtcgccggg gctcaggaga gctgggcggc gagccaatcg
    11041 aggtctgcgc cggcatggga gcgccagtag tcgccggtat gtccgccggc gtcgaagttc
    11101 gttacggcgt gcgggacgac atcgcgggcg aaggcgcggt tggccgcgat gaacgggtcg
    11161 tcggtgccgc agtcgaggcg aatcggtatg cgggagaggg cctttgcgcg tcggaagacg
    11221 tcgtgggcga cgaagtcctc gcggtcgtcg aaagcgccct ccgcgctgtc gcccgggtcg
    11281 gtccacaggg cggcgctctg ggcgacgacg ccgaggaccg tggccggctt catcgcggag
    11341 gcgagcagga gagatccgta gccgcccatc gagaacccga ggaaacccag cggcgtgctc
    11401 tccgggatct tcgcgtagct gcgcatcagt ggcaggaagt cctgcatgac catggccccg
    11461 gcatcgacgc ccgagcggcg cttgtgccaa taggtgtcgc cgccgctgat cgtcgccacg
    11521 gcgagttccg tccgggccac gtgctcgtcg agggcgagca tgtccatcat ctccgggccg
    11581 ttagatccct tgccatgcaa gccgatcacg agggcgcggg atcccttgcc catcggcatg
    11641 agcaggctcc aggccgactc ctcaggcgcc aggtgcacgg aggtcagcgt gccgtccatc
    11701 cggtggctgt gcgcgtgtgt cgtcgccgat cccgaggtcg agatcgacaa atcacaactc
    11761 gcggtcgcgg cagcggcggc cccgagcagc agggtgcgcc gcgccactgg ccggccccca
    11821 ctgctcatac cgtcatcgtg ccaccggcac cggcgagccg agcgcacgcc gctccgcggc
    11881 gaccaggcgc gagcggccca gcaacgccag gacgaaggcg agcgcggcgg cggcgacggg
    11941 cacgaggaag gcgcggtggg cgccatacga ctcgatgacc tgaccggcag cggccgagcc
    12001 gaccccgacc ccgatgagca ggccggtgac gacgatcgtc atgccctcgt tgagctgact
    12061 cgtcggaacc agcctggggc acaaggtcat cgccgtgatc agtgtcgggg cgatggcgag
    12121 gccggcgacg aagccgttca cggccagcac gggaatgttg ggaacccaca gcacaggcgc
    12181 caggagggcg aacatcgcga aggtccagac cgtcatccgg cgggcgattc gcccgtccgc
    12241 gggcacgagt ccgtagagca ggccaccgac gagggagccg gcggcgaaga agccgacgag
    12301 cgcgccgccg agggctttga cgccgttgtc ctccgcatag gccagcacca cgacgtccat
    12361 gccgccgaag accgcgccgg tcagggtgag ggtgacggcc agtccgagca gcccgggcgt
    12421 ggtgaacgcg ccgaggacgt ggtgcttctc gtcgctgacg tgcaccggcg gctcgctgac
    12481 tcggtgggcc agcaaaccga gcagcccgac caggaagagc gtcgtcgcca ccaggacgcc
    12541 ggcctcgggg aaaagccatg acgccaatgc ggcgccgagt gccggcccca ggacgaagca
    12601 cgactcctcg gcggcctgct cgaaggaggt ggcgacgtgc agatctgccg gccgctcccg
    12661 gagcagatgg gtccaccggg tgcggaccag cgtgctggga ttgggcgcga ccgcctgccc
    12721 gagggcggtg acgagcaaga cccagccggg caccccggca tacgcctcca cggtgagggc
    12781 gagcaggcag cacccgacga ccagcccggc cggcaacgcg atccggcgct gcccgaaccg
    12841 gtcgatcgcg cgcgccacga gcggcccgcc cattgccatc gagatcagcc cgacgaagga
    12901 gacgaggccg gcgagggcgt acgagtcgta gcgggtggcg atcatgacga cgatcgccac
    12961 gccgaacatc gacgtgccca gccgggcgac ataggcactg gcgaagaagt gcggcgccga
    13021 cggcatgacg cgcaggacgc ggtagggagc gaagaagcgg cgcacgatga tccattgtcg
    13081 gccgtccgtc caagtccgtc gaatcgctcg gcacctccag gtgccgggtc gcggccgagc
    13141 cgtctcgcgc cataccggaa tgacgcgccg ggcatactga gccgcatggg ttaccacggg
    13201 atgaccgagg acgaagtggc agccttcctc accgctctgc ccgcgcggcc gggtgtgctg
    13261 gcgacgacgc gcaaggacgg acggccc
//
LOCUS       DAOPJG010000082        13235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4929_length_13235_cov_7.713050, whole genome shotgun sequence.
ACCESSION   DAOPJG010000082 DAOPJG010000000
VERSION     DAOPJG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4929_length_13235_cov_7.713050"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKU75_08950"
     CDS             complement(<1..658)
                     /locus_tag="PKU75_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPF81045.1"
                     /translation="MRIATFNVNGIRAARRRGFGTWLTRTDPDVVALQEVRCPEAALP
                     DEAFGGYHAAYDAGARAGRNGVAILTREQPAAVRTLDPTGYDFTLARELRAYADQGRY
                     LEVDLADAPLTVASVYVPKGGLPAHLQIPGRMREAPDGGAAFERKLRFMAGMSRHLNR
                     SRRSAAARGREFIVMGDFNAAHREADVANWRRSKTSVGFLPAERAWLENLLGPRTFVD
                     V"
     gene            complement(679..1431)
                     /locus_tag="PKU75_08955"
     CDS             complement(679..1431)
                     /locus_tag="PKU75_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPF81046.1"
                     /translation="MERVYGPVIGLALSVFKAQGLQFTIVGEDNVPRAGGAVMAINHT
                     SYFDFTYAGLAARKSKRYVRFMAKASIWQHKIAGPLMRGMKHIPVDRHAGAASYREAL
                     TALKSGEIVGVFPEATMSRSFELKPFKSGAARMARDAGVPLLPTTLWGAHRVWTKDAP
                     KHRGRSKIPIYIEVGEPIFIPKGGDVEAANEQLRAAMQAQLDRQQAAYPTLTGDDLRY
                     LPARLGGTAPTPEDAHERDTHDMSRTQDEFNS"
     gene            1485..4952
                     /gene="recC"
                     /locus_tag="PKU75_08960"
     CDS             1485..4952
                     /gene="recC"
                     /locus_tag="PKU75_08960"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HPF81047.1"
                     /translation="MAIHLHRAARTDLLVEELASVLAVPASDPFATELVVVPAKGVER
                     WLTQRLAHRLGAANGDDGVCAGVRFLSPNRLVTLLLGTDRDDPWLPDQLVWPVLAAID
                     DNLGMPWATQLAAHLGYGDESPQGRLRAGRRYAVARRLAGLFDAYAAQRPALLRSWEA
                     AADVGPWEDGAGAPLDADLAWQPHVWRAVVDRVRLPTPVARHAQTVESLRTAPEAFDI
                     PDRLSLFGHTRLASTEVELLAAVAEHRDVHLWLPHPSESLWAAMQSVVIDGPCARADD
                     RSVLAVRHPLLASLGRDVRELQRTLALAGPMSEETIAAPAGGGIPDPATWLEWLQADL
                     RADRVPTTAEAAQRGIPVGDRSIQVHGAHGPARQVEVLREVLLGLLADDPSLEPRDIL
                     VMCPDVDTFAPLIHADFGLGDLWIGDTPGSSLHPGHHLRVQLADRSKIFTNPLLAIAA
                     QLVELAAGRITASEVLDLAATEPVRRRFGFDEDDLERFAAWTEQAAIRWGLDGRHRTE
                     FQLDGLDANSWHAGLDRLLLGVTRAEDGTRSYPFVLPLDDVDSGSIELAGRVVEFVDR
                     IAAFRDACTCAQSAAQWTAALRDGVSALTDVPFTDLWQQAEFERELAVIAGSASAITL
                     SHSDIRTLLSTRLESRATRANFRTGSLTVCTMVPMRSVPHRVVALLGLDDGVFPRAST
                     PDGDDVLARAPRTGERDARSEDRQLLLDAILAATDTLVVTYSAADPHTGAPRPPAVPL
                     GELIDAARATAGLDPTGAIGAIGADDAGDADTRPVRRHRLQPFDTENLTPDAISGVPF
                     SFDPQSVPAAQALRDGKALTASGAAPTSGLAVPGPLPMRDGDDLTVADLITFFANPTR
                     AYLRERLDVASPLEEELRPDAIPVDLDGLQSWQIGERLLSDLLAGKASVEIERAELQR
                     GSLPPAALGYRLLVDIGPRADAIAAATGRILTDSPVAARSVDIDVEIGGGRRLVGSVQ
                     RVHDRAFVLPTYSTLSGKHRLEAWITLLALTAGTPAGAGPWTAHAIGRRGKDSCVANV
                     GPIAPDLARTFLLELVELRDLGLSMPLAFAPKTSYDYAARLHQLKGRKETLALAAARE
                     QWEKRFDNNDPSIALLYGPNPPLDIWLGPPSADEVWEFAGEAESLPSRLGHTAMRVFR
                     PALLAGVGT"
     gene            4949..8311
                     /locus_tag="PKU75_08965"
     CDS             4949..8311
                     /locus_tag="PKU75_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPF81048.1"
                     /translation="MSAPTATAPVFDITAPVPAGTTILEASAGTGKTWAIAALVARVV
                     AEGTSLDQILVVTFGRAATQELRERVRERLIRSELYLRQLIDGHEPARRIDRLEKTLS
                     GDASGTRFDVAELRVRHHRLRDALGAFDDATIATIHQFCHSVLRSLGVAGDTDPGTTL
                     VEDLSDLLAEVVDDLYLARYARLDAVPFSHKTAREIATKVVGDPAARIGPADAEPGSV
                     EAERVAFAEEVRSELAYRKRRLGVLSYDDLLADLAAALEDPRSPTRARLRERWSVVLV
                     DEFQDTDPVQWQVFERAFHGHGTLVLIGDPKQAIYAFRGGDINTYLRARQVADHQQTL
                     AVNRRSDAPLVAALSAGLGGMTLGDPAVEVHPVTAAQHESRLSGLPNAQPWRLRVASR
                     AMLDTDPDRLARVDKARPILAKDCATDIARVLSSAATFDGRPVAPGDIAVLCHTGKQL
                     RHVRDALQAAGIPAVLAADDSVLRSPAAKWWLMLLEAMEMPNRSRRVRTAALTPFIGE
                     SITALDQRGEVLTDDVAAQLRRWAELFALRGIPAVVAAARAGGLDQRLLATAGGERSR
                     TDVHHLAEVLHDHALRERLGLNATTTWLRRQLAEEAAPGLGHRSRRLESDAAAVQLAT
                     IHASKGLQFPIVYAPFLADRFDSDDAGFLRFHDTAGNRVIDVRDRADANTLERAKAED
                     DGESLRMLYVALTRAQSQVVTWWAPTTNTPTSPLHRALLGRSPGGHGEPERSLPVPSE
                     RIVTERLGAWAAAGGPQPEWVEPGPATLPATPPPGADDLAVRTFDRQVDVHWRRTSYS
                     ALSKAAEDAAGVPATSEPELPGKDDEAPGVEILPVGDTPTPAELLAADVPSPMATLPV
                     GATFGSLVHAVLEEADPAAGDLRAEFLARIEEQIVRWPVELNRATLADALVAVSRSPL
                     GPLASGAALADIGRSDRLCELEFELPLVGGDHRRNVGDIRLGELAPLLRAHLPEGDPL
                     LDYAAALAAPELGDQVLRGYLTGSVDVVLRVGDRYLVVDYKTNWLGAPDAELTAADYR
                     PRALVAAMQHSSYPLQALLYAVVLHRFLRWRLPGYDPVRHFGGVLYLYLRGMCGPDTP
                     VIDGHPTGVFSWQPPVALVTALSDLLDGRAAS"
     gene            complement(8338..9417)
                     /locus_tag="PKU75_08970"
     CDS             complement(8338..9417)
                     /locus_tag="PKU75_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81049.1"
                     /translation="MTARATPEAMPDTIRTRHLTLRPPVPDDDDFLYALHSDPALYGH
                     APWARVTDREELEAVTAAWRATWQEHGFGYFVADDADTGEPLGMAGVRPADPGRLNLA
                     YRFAAAARGRGLGRESARAVVVFAAEWLPDHVVEAVIRPGHEASIRTAEAAGLLSVGE
                     ISHAGDPAEAGVSLLLEAPRAGRVDEIGPTDREELLDLWQAVTEAGGAVGFLPGAPRD
                     RIAQALAEHEADMRAGRSFAVGLRDPDASLVGWGWWVSPANPLLAHRRWLYRFMVDSR
                     RQGRNLGAILLASMIGTAREDGGELLGLDYRSGTGVGDFYARQGFVEVGRIPGGIRVG
                     AGDDRDDVEMVRRADGSPLVADGRR"
     assembly_gap    9540..9639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9709..11463
                     /gene="recD"
                     /locus_tag="PKU75_08975"
     CDS             9709..11463
                     /gene="recD"
                     /locus_tag="PKU75_08975"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HPF81050.1"
                     /translation="MPAGLIADANEFGILHAADVHVARRLAAMARESDPEVVLALALA
                     TRAVRTGSVAVDLHALAAVDPDFAWPADVADWLARVAASPLVTTGVLRLDDGLLYLER
                     YHDQEVLVAETLGTRRALHPIPVHESELAAGLGRLFPDPRDADQRAAAELAVRERTAV
                     ITGGPGTGKTTTIARILALLAEQSTLTDGFHVPRFALAAPTAKAAARLQDAFATAAAG
                     LPDSDRERLPIPAASTLHRLLGWRPGSRSRFAHDAATRLPHDVVVVDEASMVSLTMMA
                     RLLEALRPTARLIVVGDPDQLTSVEAGAVLADLVAGLDRDPAQPAGSSAVARLRHTYR
                     FGGAIQDLATAIRDGDATAALAILTSGDPAVELVPNADARRSLMVEAAVDLRRHGLAG
                     DARAALAALGEHRLLCAHRTGPQGVGHWNRQIAQWLSEETGENFYAPMFPGRPLQVTA
                     NDYAMDLYNGDTGVVLRRDGRLVAVFDSGSQLREVAAARLADVETMYAATVHKSQGSQ
                     ATRVTVVLPELDSPLLTRELIYTAATRAQKQLTIIGTPEVIERGMAQRIQRASGLAKR
                     LRRPPPAVPGEVSAGSPG"
     gene            11478..>13235
                     /locus_tag="PKU75_08980"
     CDS             11478..>13235
                     /locus_tag="PKU75_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81051.1"
                     /translation="MDSLAGADWLGELTDEAIRARVDAGGFARGQQYAASGMVLSLTT
                     ADRGRMILAEVEGSRSRPYQTLITVAPTGRNAAAWTSRCSCPVQMNCKHSVAVLLVAR
                     QRYREAGGHDTPQAPWEATLRKLIEERTEVIDTAPGLGLIVDPLTVASNYRTMMGPPR
                     IAIRVTRRTKTGSWARNFTWTEISSPHSRMPMNRRHAAALKAIHDLHRLTGGRDGYFY
                     SQGEIYLGALGSRVWPLLKAAVEAGVELVGGGNVVGDVSLAPEPARIVLDVTRDDAEI
                     VLTTSVDTATALGEVRAINVLGDPAHGLAVTTRDGLVLLGLEQELDENLVELLVHQRQ
                     LRIPGSDTERFVNLYLPRLRDHARVTSHDDSVSIPEQPRPLLRVRVGQPMPTHIELRF
                     DIAYAAPNGELVTADDGVALPRDRTREQSLLDGLEVLEQIPGARTRRGTAYSGYWDLA
                     NRLVLHGMPAVRFMSDVLPALEFEPDVHVVVEDGLTAFEEAVERPVIDVSLSDSEDGR
                     TDWFDLRLAVSVGQESVPLEQLLRALAAGDEALLLDSGTWFRLDDPALDSLRNLIAEA
                     RQLVEPGAPLRLTRYHVGLW"
BASE COUNT         1820 a         4934 c         4471 g         1910 t
ORIGIN      
        1 ccacatcgac gaaagtcctt ggcccgagca ggttttcgag ccacgcccgc tcggccggca
       61 agaaccccac cgaggtcttg ctgcggcgcc agttggcgac gtcggcctcc cggtgggcgg
      121 cgttgaagtc gcccatgacg atgaactccc gaccccgcgc cgcagcggag cgccgggagc
      181 ggttcaggtg ccggctcatc ccggccatga accgcagctt gcgctcgaac gctgccccgc
      241 cgtcgggtgc ctcgcgcatg cgccccggga tctgcaggtg cgccggcagc ccgcccttgg
      301 ggacatagac cgaggcgacg gtcagcggcg cgtcggcgag gtcgacctcg aggtagcgcc
      361 cctggtcggc atacgctcgc aactcgcggg cgagggtgaa gtcgtatccg gtggggtcga
      421 gggtgcgcac cgcggcgggc tgctctcgcg tgaggatggc gacaccattg cggccagctc
      481 gcgcccctgc gtcgtatgcc gcgtggtagc ccccgaacgc ctcatccggc agcgctgcct
      541 cggggcagcg aacctcctgc agcgcaacga catccgggtc ggtccgcgtc aaccaggtgc
      601 cgaacccgcg tcgccgcgcc gcgcggatcc cgttgacgtt gaaagtggcg atccgcaccg
      661 cgtgatcgta cgcggggtct aggagttgaa ctcgtcctgc gtgcggctca tgtcgtgggt
      721 gtcccgctcg tgggcatcct cgggggtggg ggcggtgcca ccgagccggg cggggagata
      781 acgcaggtcg tcgccggtca acgtcggata ggccgcctgc tgccggtcga gctgggcctg
      841 catcgccgcg cgcagctgct cgttggcggc ctcgacgtcg ccgcccttgg ggatgaagat
      901 cggctccccc acctcgatat agatcgggat cttgctgcgg ccccggtgtt taggcgcgtc
      961 tttggtccat acccggtggg caccccagag cgtcgtcggc aacagcggca cgccggcgtc
     1021 gcgcgccatc cgcgccgcac cggatttgaa cggcttgagt tcgaaggacc gcgacatggt
     1081 cgcctccggg aaaaccccga cgatctcccc cgacttcagt gccgtcagcg cctcccggta
     1141 ggatgcggcc cccgcgtgcc ggtcgaccgg gatgtgcttc ataccccgca tgagcggccc
     1201 ggcgatcttg tgctgccaga tcgaggcctt ggccatgaag cggacatacc gcttgctctt
     1261 gcgcgctgcc agcccggcgt aggtgaagtc gaagtagctg gtgtggttga tcgccatcac
     1321 cgccccgccc gcacgcggca cgttgtcctc gccgacgatc gtgaactgca ggccctgggc
     1381 cttgaacacc gacagggcca agccgatgac gggaccgtag acgcgctcca tggggcaagg
     1441 ctacggctca ccaccttgtc ggtggcggaa tctagggtga gtccgtggcc atccacctgc
     1501 accgcgcggc gcggaccgat ctcctcgtgg aggagctcgc gtccgtgctc gccgtgccgg
     1561 cgtccgatcc cttcgccacc gagctggtcg tggtgcccgc taaaggggtc gagcgctggc
     1621 tgacccagcg gctggcgcat cgcctcgggg ccgccaatgg cgacgacggg gtgtgcgcgg
     1681 gggtgcgttt cctctctccc aaccgcctgg tcaccctgct gctcggcacc gatcgcgacg
     1741 atccgtggct gcccgatcag ctcgtctggc ccgtgctcgc cgccatcgac gacaacctcg
     1801 gtatgccgtg ggccacccaa ctcgctgcgc acctggggta tggcgacgag agtccccaag
     1861 gccggttgcg cgccggccgg cggtatgccg tggcgcgccg cctcgcgggg cttttcgatg
     1921 cgtatgccgc gcaacgcccc gccctgctgc ggtcgtggga ggccgcagca gacgtggggc
     1981 cgtgggagga cggcgcgggt gcgccgctcg atgccgatct cgcgtggcag ccgcacgtgt
     2041 ggcgggccgt ggtcgaccgg gtcaggctgc cgacgccggt ggcgcggcac gcccagacgg
     2101 tcgagtcgtt gcgcacggca cccgaggcgt tcgacatccc ggaccggctc tccctgttcg
     2161 gtcacacgcg gctggcgtcg accgaggtcg agctcctggc cgccgtggcc gaacaccgcg
     2221 acgtgcatct gtggctgcca catccgtccg aatccctttg ggcggcaatg caatcggtcg
     2281 tcattgatgg gccgtgcgcc cgcgctgacg atcgctccgt ccttgcggtg cggcacccgt
     2341 tgctcgcctc gctcgggcgc gacgtccgcg aactgcagcg caccctcgcc cttgccggcc
     2401 cgatgagcga ggagacgatc gccgcgccgg ccgggggcgg cataccagac ccggcgacct
     2461 ggctggagtg gttgcaggcc gacctgcggg ccgaccgggt gccgacgacg gccgaggccg
     2521 cccagcgcgg catacccgtg ggtgatcgca gcatccaggt gcacggtgcg cacggcccgg
     2581 cccggcaggt cgaggtgctc cgggaagtgc tgctcgggct gctcgcggac gatccatcgc
     2641 tcgaaccccg cgacatcctc gtcatgtgcc ccgacgtcga caccttcgcc ccgctgatcc
     2701 acgccgactt cgggctgggc gacctgtgga tcggcgacac ccccggcagc agcctgcatc
     2761 ccgggcacca cctgcgcgtg caactcgccg accggtccaa gatcttcacc aacccgttgc
     2821 tcgcgatcgc agcccaactc gtcgaactcg ccgccgggcg gatcacggcg agcgaggtgc
     2881 tcgacctggc agcgaccgaa ccggtgcgcc gccggttcgg gttcgacgag gacgacctcg
     2941 agcgcttcgc ggcgtggacc gagcaggccg cgatccgctg gggcctggac gggcggcacc
     3001 gcaccgaatt ccagctcgac ggactcgacg ccaactcgtg gcacgccggg ctcgaccgcc
     3061 tgctgctcgg ggtgacccgc gccgaggacg gcacgcgcag ttatcccttc gtcctgcccc
     3121 tcgacgatgt cgacagcggc agcatcgaac tggccggccg cgtcgtcgaa ttcgtcgacc
     3181 ggatcgccgc gttccgcgac gcctgcacgt gtgcccagag cgccgcccaa tggaccgccg
     3241 ccctgcgcga cggggtgagc gcgttgaccg atgtgccctt caccgacctg tggcagcagg
     3301 ccgagttcga acgcgagctt gccgtcatcg ccgggtcggc aagcgcgatc accctgtctc
     3361 acagcgatat ccgcacgctg ctcagcaccc ggctggagtc tcgcgcgacg cgcgccaact
     3421 tccgcaccgg cagcctgacc gtgtgcacga tggtgccgat gcgttcggtc ccgcaccggg
     3481 tcgtggcgct gctcgggctg gacgacggtg tcttcccccg cgccagcacc cccgacggcg
     3541 acgacgtcct ggcccgggcc ccgcgcaccg gggagcgcga cgcccgcagc gaagaccggc
     3601 agctgctcct cgacgcgatc ctcgcggcca ccgacacact ggtcgtgacc tattcggcgg
     3661 ccgatccgca tacgggggca ccccggcccc cagcggtgcc gctgggcgaa ctcatcgacg
     3721 cggcgcgcgc cacggcgggc ctggacccca ctggtgccat tggtgccatt ggtgccgacg
     3781 atgccggcga cgccgacacc cggccggtgc gccgccaccg gctgcaacca ttcgacaccg
     3841 agaacctcac ccccgacgcg atctccggcg tgccgttcag cttcgatccg cagtccgtgc
     3901 cggccgccca ggcgctccgg gacggcaagg ccctgaccgc gtccggggcc gcgccgacct
     3961 caggtctcgc ggtgcccggt ccgctgccca tgcgtgacgg cgacgacctg accgtcgccg
     4021 acctgatcac cttcttcgcc aaccccactc gcgcctatct gcgtgagcgg ctcgatgtcg
     4081 cctccccgct cgaggaggag ttgcgccccg atgcgatccc ggtcgatctc gacggcctgc
     4141 agtcctggca gatcggagag cggctgttgt cggacctgtt ggcaggcaag gcatccgtgg
     4201 agatcgagcg cgccgaactg cagcgtggca gcctgccccc cgcggccctc ggctaccggc
     4261 tgctggtgga catcggaccg cgtgccgacg cgatcgccgc agcgaccggg cgcatcctca
     4321 ccgactcccc cgtggcggcg cggtcggtcg acatcgatgt cgagatcggc ggtggccggc
     4381 gcctcgtcgg gagcgtccaa cgggtgcacg accgggcctt cgtgctgccg acctattcca
     4441 ctctctcggg caagcaccgg ctcgaagcct ggatcaccct gctggccctc acggccggca
     4501 cgcccgccgg cgccgggccg tggaccgcac acgcgatcgg acggcggggc aaggactcct
     4561 gcgtcgccaa tgtcggcccg atcgcccctg acctggcgcg caccttcctg ctcgagctcg
     4621 tcgagctgcg cgacctgggg ctgtcgatgc cgctggcatt cgcgcccaag acgtcctacg
     4681 actacgccgc ccggctccat cagctcaagg gacgcaagga gacgctggcg ctggcggcgg
     4741 cacgagagca gtgggagaag cggttcgaca acaacgaccc gtccatcgcc ctcctctacg
     4801 gccccaatcc gccactcgac atctggctcg gcccgccgag cgcggacgag gtatgggagt
     4861 tcgccggcga ggccgagagc ctgccctcgc ggctcgggca tacggccatg cgcgtcttcc
     4921 ggccggcact gctcgcgggg gtgggcacgt gagtgcccca accgccaccg ccccggtctt
     4981 cgacatcacc gcgcccgtgc ctgccgggac caccatcctc gaagcgagcg cgggcaccgg
     5041 caagacctgg gcaatcgctg ccctggtcgc gcgtgtcgtt gccgagggca cttcgttgga
     5101 ccagatcctc gtcgtcacct tcggccgggc ggccactcag gagttgcgcg aacgggtccg
     5161 cgaacggctg atccgctccg agctctatct gcgccagctc atcgatggtc acgagccggc
     5221 ccggcgcatc gaccggctcg agaagaccct cagcggcgat gcgagcggca cccgtttcga
     5281 cgtggcggag ctgcgcgtcc gccaccatcg cctccgcgac gcgctcggtg ccttcgacga
     5341 cgcgacgatc gcgacgatcc accagttctg tcattcggtg ttgcgcagcc tcggggtggc
     5401 cggtgacacc gatcccggca cgacgctggt cgaggatctg agcgatctgc tggccgaggt
     5461 cgtcgacgat ctctacctcg cgcgctatgc ccgactcgac gcggtcccgt tctcgcacaa
     5521 gaccgctcgc gagatcgcga cgaaggtcgt gggcgatccg gccgcgcgta tcgggccggc
     5581 cgacgccgag cccggcagcg tcgaggccga acgggtggcc ttcgccgagg aggtccggtc
     5641 cgagctggcc taccgcaagc gccgcctggg ggtgctgtcc tatgacgact tgctcgccga
     5701 cctggccgcg gcgctcgaag atccgcggtc gcccacccgc gcgcgactgc gcgaacgctg
     5761 gtccgtggtg ctggtcgacg agttccagga caccgacccc gtgcagtggc aggtcttcga
     5821 gcgggccttc cacgggcacg gcacgctggt gctcatcggt gacccgaaac aggcgatcta
     5881 cgccttccga ggtggcgaca tcaacaccta cctgcgggcc cggcaggtcg ccgatcacca
     5941 gcagaccctg gcggtcaacc ggcgctccga cgctcctctc gtcgccgcgc tgagcgcagg
     6001 actcggcggc atgacgctcg gggacccggc ggtcgaggtc catcccgtca cggcggccca
     6061 gcacgaatcg cggctgagcg gactgcccaa tgcccagccc tggcggctgc gggtggcctc
     6121 ccgggccatg ctcgacaccg accccgaccg gctcgcccgg gtcgacaagg cccggcccat
     6181 cctggccaag gactgcgcca ccgacatcgc ccgggtgctg tccagcgcag ccaccttcga
     6241 cggccgcccg gtggctcctg gcgatatcgc cgtgctctgc catacgggca agcagctgcg
     6301 tcatgtccgc gacgccttgc aggccgccgg catcccagcc gtcctggccg ccgacgactc
     6361 cgtcctccgc tcgcccgcag ccaagtggtg gctcatgctc ctcgaggcca tggagatgcc
     6421 caaccggtcg cggcgcgtgc ggaccgcggc tctgacgccc ttcatcggcg agtccatcac
     6481 cgcgctcgat cagcggggcg aggtcctgac cgacgacgtt gccgcccagc tgcggcgctg
     6541 ggccgagctg ttcgcgctgc gcggcatacc ggcggtggtc gcagccgcgc gcgccggcgg
     6601 gctggaccag cgcctgctgg ccaccgcggg tggtgagcgg tcccgcaccg acgtccatca
     6661 cctcgccgag gtgttgcacg accacgcgct gcgcgagcga ctgggcctga acgcgacgac
     6721 gacatggctg cgccgccagc tcgccgagga agcggcaccg ggcctcggcc atcgcagccg
     6781 ccgactcgaa agcgacgccg ccgcagtgca actcgcgacg attcacgcga gcaagggcct
     6841 gcagttcccg atcgtctacg cgcccttcct cgcggaccgc ttcgactccg acgacgccgg
     6901 cttcttgcgg ttccacgaca ccgcgggcaa ccgcgtcatc gacgtgcgcg accgcgccga
     6961 cgccaacacg ctggagcggg ccaaggccga ggacgacggc gagagcctgc ggatgctcta
     7021 cgtcgccctc acgcgcgccc agtcccaggt cgtcacctgg tgggccccga ccacgaacac
     7081 cccgacctcc ccgctgcacc gggcgttgct cggccgctcc cccggcggcc acggcgaacc
     7141 cgagcggagc ctgccggtcc cgagcgagcg catcgtcacc gagcgcctcg gtgcctgggc
     7201 ggccgcgggc gggccacaac cggagtgggt cgagccgggg cccgcgacgc tccccgcgac
     7261 gccgccaccg ggcgcggacg acctggcggt gcgcacgttc gatcgccagg tggatgtgca
     7321 ctggcggcgc acctcataca gcgcgctgtc caaggccgcc gaggacgccg ccggcgtgcc
     7381 cgccacgtcc gaacccgagc ttccgggcaa ggacgacgaa gcgcccggtg tcgagatcct
     7441 ccccgtcggc gacacgccga cgccggcgga gcttctcgcc gccgacgtcc cgtcacccat
     7501 ggcgacgctt cccgtcgggg cgacgttcgg ctcgctcgtg cacgcggtgc tcgaggaggc
     7561 cgatcccgct gccggcgatc tgcgggcgga gttcctggcg cggatcgagg agcagatcgt
     7621 gcggtggccc gtcgagctca accgcgccac cctggccgac gccctggtcg ccgtcagccg
     7681 cagccccctc gggccgctcg cctccggggc cgccctcgcc gacatcggac gctcggatcg
     7741 cctgtgcgag ttggagttcg agctcccgct tgtcggtggt gatcaccggc gcaatgtcgg
     7801 tgacatccgg ctcggtgagc tggcgccgct gctgcgggcc cacctgcccg agggcgatcc
     7861 gctcctcgac tacgccgcag cgctcgcggc tcccgaactc ggtgaccagg tgctgcgcgg
     7921 atacctgacc ggctccgtcg acgtggtcct ccgggtcggc gaccgttatc tcgtcgtcga
     7981 ctacaagacc aactggctcg gtgcccccga tgccgaactc acggccgccg actaccgccc
     8041 ccgggccctg gtcgccgcga tgcagcactc gtcctatcca ctgcaggcgc tgctgtatgc
     8101 cgttgtcctg caccgcttcc tgcgttggcg actgcccgga tacgaccccg tccgccactt
     8161 cggtggcgtg ctctatctct acctgcgcgg catgtgcgga cccgacacgc ccgtcatcga
     8221 cggacacccg acgggggtct tctcctggca gccgcccgtg gcgctcgtga ctgcgctgtc
     8281 cgatctgctc gacgggcggg ccgcgtcatg atgcgcgggt gtgacagccc gtcggcgtca
     8341 gcggcggcca tccgcgacca gcgggctccc gtcggctctg cggaccattt ccacgtcgtc
     8401 gcgatcgtct cccgcaccca cccggatgcc gcccgggatc cggccgacct cgacgaaccc
     8461 ctggcgggca tagaagtcgc ccaccccggt tccgctgcga tagtccaatc cgagcagttc
     8521 accgccgtcc tcgcgtgccg tgccgatcat cgacgccaac aggatggcgc cgaggttgcg
     8581 gccctggcgg cgggagtcga ccatgaagcg atagagccaa cggcgatgtg ccaaaagcgg
     8641 attcgccgga ctgacccacc agccccaccc gacgagagag gcatcggggt cgcgcagacc
     8701 cacggcgaac gaccggcccg cgcgcatgtc cgcctcgtgc tcggcgaggg cctgcgcgat
     8761 gcggtcgcgc ggcgcgcccg gaaggaaacc cactgcaccc ccggcctcgg tgaccgcttg
     8821 ccacaggtcg agcagttctt cgcgatcggt ggggccgatc tcgtcgacgc ggccggctcg
     8881 gggtgcttcg aggagcagcg agactccggc ctcggccggg tccccggcgt gcgagatctc
     8941 gccgacggac agcagcccgg cggcctccgc cgtccggatc gacgcctcgt gaccagggcg
     9001 gatgaccgcc tccacgacat ggtccggcag ccactccgcg gcgaagacga cgaccgcacg
     9061 agcggactca cgcccgagac cacggccccg agctgccgcg gcgaaccggt aggccaggtt
     9121 gagtcgtcca ggatcggcgg gacgcacgcc cgccatgccc agcggctccc ccgtgtcggc
     9181 gtcgtccgcg acgaagtagc cgaagccgtg ctcctgccag gtcgcgcgcc acgccgcggt
     9241 caccgcttcc agctcttctc ggtcggtgac cctggcccag ggggcatgac cgtacagcgc
     9301 tggatcggag tgcagggcat agaggaagtc gtcgtcgtcc gggaccggcg gccgcagggt
     9361 caggtgccgg gtgcggatgg tgtcgggcat ggcctcgggt gtggccctgg cggtcatggc
     9421 gccacgctat ccgggcccac cgaccgcccg agcggccggc cacgtggggg tgcgcgatga
     9481 ccgagagccc ggcaccccgc gaatcactcc cccgggaacc actccctcgc gaaccactcn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aaccactccc tcgcgaacca
     9661 ctctcccgcg atccactccc tcgcaacgat ccccgccgcg tctcccggct gccggccggc
     9721 ctcatcgcgg acgcgaacga gttcgggata ctgcacgccg ccgacgtcca cgtcgcccgc
     9781 cgcctcgcgg ccatggcccg cgagagcgac ccggaggtcg tcctcgcgct ggccctcgcg
     9841 acccgggccg tccgcaccgg ttcggtggcc gtcgacctgc acgcgctcgc cgcggtcgac
     9901 cccgacttcg cgtggccggc cgacgtcgcg gactggctgg cccgcgtcgc cgcgagcccg
     9961 ctcgtcacga ccggggtgct gcgcctggac gacggcctgc tctatctgga gcgctaccac
    10021 gaccaggagg ttctcgtcgc cgagaccctg ggcacgaggc gagcgctgca cccgatcccg
    10081 gtccacgaga gtgagctggc cgccggcctc ggccggctgt tcccggatcc gcgcgacgcc
    10141 gaccagcggg cggccgcaga gctggccgtc cgcgaacgca ccgccgtgat caccggtggc
    10201 cccggcaccg gcaagacgac gacgatcgcc cgcatcctgg cgctgctggc cgagcagtcc
    10261 accctcaccg acggcttcca cgtgccgcgg ttcgcgctcg cggcacccac cgccaaagcc
    10321 gctgcccggc tccaggacgc cttcgcgacc gcagcggccg gcctgcccga cagcgaccgc
    10381 gagcgactgc ccatacccgc ggcctccacc ctgcaccgcc tcctcggctg gcggccggga
    10441 tcacgcagcc gcttcgccca cgacgccgcc acccggctcc cccacgacgt cgtcgtcgtc
    10501 gacgaggcgt ccatggtgtc cctgacgatg atggcgcgcc tgctcgaggc cctgcgcccg
    10561 accgcccgcc tcatcgtcgt cggggacccc gaccagctca cctcggtcga ggccggcgcc
    10621 gtcctggccg acctcgtcgc cgggctggat cgtgacccgg cgcagccggc aggctccagc
    10681 gcggtggcgc ggctgcggca tacctatcgg ttcggcggtg ccatccagga tctggccacg
    10741 gcgatccgcg acggcgacgc caccgcggcc ctggccatcc tgacgtccgg agacccggcc
    10801 gtcgaactgg tccccaatgc cgatgcccgg cgctcgctca tggtggaggc ggcggtcgac
    10861 ctgcgtcgcc acggactggc cggcgacgcc cgcgccgcgc tggccgcgtt gggtgagcac
    10921 cgcctgctgt gcgcgcaccg caccggcccg cagggggtcg gccactggaa ccggcagatc
    10981 gctcaatggc tctcggagga gaccggcgag aacttctacg cccccatgtt cccgggccgg
    11041 ccgctgcagg tgaccgccaa cgactacgcc atggacctct acaacggcga caccggggtc
    11101 gtcctgcgcc gcgacggccg cctcgtcgcc gtcttcgatt ccgggtcaca gctgcgcgag
    11161 gtcgccgccg cccgcctcgc cgacgtcgag acgatgtatg ccgcgacggt gcacaagagc
    11221 cagggcagcc aggcgacacg cgtcaccgtc gtcctgcccg aactcgactc gccgttgttg
    11281 acccgcgagc tgatctacac cgccgccacc cgcgcgcaga agcaactgac gatcatcggc
    11341 actcccgagg tcatcgagcg cggtatggcg cagcggatcc agcgcgcatc cggcctcgcc
    11401 aagcggttgc ggaggccgcc gccggccgtt cccggtgagg tttcggcggg ttcgccgggt
    11461 tgagttatgt tgggatgatg gactcattgg ccggggctga ttggctcggc gagctgaccg
    11521 acgaggcgat ccgcgcccgc gtcgacgcgg gcgggttcgc gcgcggccag cagtatgccg
    11581 cgtccggcat ggtcctctcg ctcaccacgg ccgaccgcgg ccggatgatc ctcgccgagg
    11641 tcgaggggtc ccgctcgcgg ccctatcaga ccctgatcac ggtggcgccg accggccgca
    11701 acgcggcggc ctggacgtcc cgatgcagtt gcccggtgca gatgaactgc aagcactcgg
    11761 tggcggtgct gctcgtcgcg cgccagcgct atcgcgaagc cggtggtcac gacaccccgc
    11821 aggcgccgtg ggaagccacg ctccgcaagc tcatcgagga gcgcaccgag gtcatcgaca
    11881 ccgcccccgg gctcgggctg atcgtcgacc cgctaaccgt ggcctcgaac tatcgcacga
    11941 tgatgggtcc gccgcgcatc gccatccgtg tcacccggcg caccaagacc gggtcgtggg
    12001 cgcgcaactt cacgtggacc gagatcagtt cgccgcattc ccggatgccg atgaaccggc
    12061 ggcacgccgc tgcgttgaag gccatccacg atctgcaccg cctgaccggt ggccgggacg
    12121 gctacttcta cagccagggc gagatctatc tcggcgccct cggatcacgg gtctggccgt
    12181 tgctcaaggc cgccgtcgaa gccggggtcg aactcgtggg gggtggcaac gtcgtcggcg
    12241 acgtcagcct cgcacccgaa cccgcccgga tcgtcctcga cgtcacccgc gacgatgccg
    12301 agatcgtcct gacgacgtcg gtggacacag cgacggccct cggggaagtg cgggccatca
    12361 acgtcctggg cgacccggcc cacgggctcg ccgtcaccac gcgcgacggc ctggtgctgc
    12421 tcgggctgga gcaggaactc gacgagaacc tcgtcgaact ccttgtccat cagcgacaat
    12481 tgcggatccc ggggtccgac accgagaggt tcgtcaacct ctacctgccc cggctgcgcg
    12541 atcacgcgcg ggtgacgagc cacgacgaca gcgtgagcat tccggagcaa ccgcgtccct
    12601 tgttgcgagt acgggtggga cagccgatgc cgacgcacat cgagctgcgc ttcgacatcg
    12661 cgtatgccgc gcccaatggg gaattggtga cggctgacga cggggtggcg ctgccgcggg
    12721 accggacgcg cgagcagtcc ctgctcgacg gtctcgaggt gctcgagcag atcccggggg
    12781 cgcggacgcg gcgcggcacg gcatacagcg gctattggga cctggcgaat cgtcttgtgc
    12841 tgcacggcat gccggcggtg cgcttcatgt ccgacgtgct gccggcgctg gagttcgagc
    12901 ccgatgtgca tgtcgtggtc gaggacgggc tcaccgcctt cgaagaagct gtcgagcgtc
    12961 cggtcatcga cgtctcgctg tccgactccg aggacggacg gaccgactgg ttcgacctgc
    13021 ggctggcggt ctccgtcggg caggagagcg tgcccctgga gcagttgttg cgggcgttgg
    13081 ccgccgggga cgaggcgttg ctgctcgact cgggcacctg gttccggctc gacgacccgg
    13141 ccctggacag cctgcgcaac ctcatcgcgg aggcgcggca gctcgtcgag cccggggccc
    13201 ccttgcggct gacgcgttat cacgtcggcc tctgg
//
LOCUS       DAOPJG010000083        13208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_4943_length_13208_cov_8.247624, whole genome shotgun sequence.
ACCESSION   DAOPJG010000083 DAOPJG010000000
VERSION     DAOPJG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4943_length_13208_cov_8.247624"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5757)
                     /locus_tag="PKU75_08985"
     CDS             complement(<1..5757)
                     /locus_tag="PKU75_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPF81052.1"
                     /translation="MTNTPKPVAVVGMAAIMPQAPSANAFWDNIKGGRYSVTEVPPER
                     WDPALYFSPDHGARDKTYSTIGGWVREFDWDPIKWRLPVPPTVAAQMDEGQRWAISAA
                     RAALIDAGWPEWNVDSNNVAVILGNAIGGEKHYLSSMRVRLPEVIKRLQESATLQGLP
                     ADVQQRIVEETRAGYLDNTFEITEDTMPGELANVIAGRVCQLLNLRGPNFTTDAACAS
                     GLAALNSAILGLVDHQYDAVVTGGVDRNMGIDAFVKFCKIGALSATGTRPFDSGADGF
                     VMGEGAALFVLKRLEDAERDGDRIYSVIMSVGGSSDGKGKGITAPNPIGQQLAVRRAW
                     EQAGLDPATVMAVEAHGTSTRVGDAAELASLTEVFGSSNLAPGSVALGSVKSNIGHLK
                     AAAGTAGLFKMVRSLHEKVLAPSLNFNNPNENVDWGSIPFKVNTSLRAWPDSPHGVRR
                     GAVSAFGFGGTNFHVVLEEHIPGRHKAPAKVFASAEVPSAATVSAPVAGPGAAGGPAK
                     APLRGALVLGGRDDAEVLGKVREALAAAQAGTAPAPAAPDRSVKDAAVRVAVDYGDAA
                     ELAGKLDKLVKAMASGAPAAFKLLRQQGVFVGRGPAPKVAFLYTGQGSQYVNMLKDLA
                     ANEPVVKATFDEADRVMTPLLGGRPLTSYIFIDGDDKDAVDQLNKQLMQTEITQPAVL
                     ATDLSLTRLLAAYGVRPDMVMGHSLGEYGALVAAGSLSLESALEAVSARGNEMKKVSV
                     ADNGAMAAMFGPLTAIEELVKTADGYAVVANINSYNQAVVGGATTAVEDLVEKAKAAG
                     MSATRIPVSHAFHTAIVAPASGPLKDALRRLNVVGPTVPCVANVTGEFYPLGASTDVM
                     LEILGQQVASPVQFVKGLETLYAAGARVFVEVGPKKALHGFVEDVLGSKHDDVTALFT
                     NHPKLSDATAVNQALCGLYAAGVGFPEPAPAAAPVAAPPTPAAAPAAAPAAAPAAPTA
                     PAAPTAYPQSTGGTTVSDNTIAELGKLLAGVLDQGLQLYAADLGYSKGAAAVVGLPAP
                     AAASGGAPTTGGEPVVITGAALGLPGVEKMFDDANVARILAGENFISVLPHEVRALIA
                     DKRVTRIVKDAHGGGSFQTIDDVADVIKLAGIHAPIDVVAEFGLDKARDEALDVTTRM
                     AVAAGFDALRDAGIPLVMRYKKTTLGTQLPDKWLLPEALRDTTGVIFASAFPGYDRFA
                     EEIEKYALHRGRRDNLLALEGVRARMTADDPARAEVDRLIGALRQALEAEPYAFDRRF
                     LFRVLAMGHSQFAEIIGARGPNTQVNAACASTTQAVSLAEDWIKAGRCERVIVVSADD
                     ASSEHLLPWVTTGFLATGAAATDEKVEDAATPFDKRRHGMIVGTGAAAIVVESAESAR
                     ERGLAPIAQVLASITANSAFHGTRLDVDHIGGVMQSLIAEAEQRGIDRHAIAPATMFV
                     SHETYTPARGGSASAEINALRQVFGASADRIVITNTKGFTGHAMGAGIEDVVALKALE
                     TGIVPPVPNYKEPDPDLGALNLSRGGTYPVEYALRLAAGFGSQIAMSLIKWVPPADGR
                     RRAPQELGYAYRVIDQAAWQTWLDTLSGHTGSALEVDHRRLRVVDNGAPAKPVTLTTR
                     VPVPYAGRLAPLGARAGAAAPAAAPAAPAPVSMPSAPAAPAAPAPAPAAPAPAASTPA
                     APAPAPAAPAAPAAPPADEILERVTGIVSEMTGYPPDLLDPDLDLEADLGVDTVKQAE
                     VFAAVRGSWDLERDENLKLRDFPTLRHVAGWVRSKLGLPEPSAAAPAASPVAAVPAAP
                     AAPAAAPADEILDRVTGIVAEMTGYPSDLLDPDLDLEADLGVDTVKQAEVFAAVRGSW
                     DLERDENLKLRDFPTLRHVAGWVRGKLGLPGPSAAAAAPAAAAPAAAAAPAPAAPAPA
                     GAPAGDEVLAKVTEIVAEM"
     gene            complement(5793..7499)
                     /locus_tag="PKU75_08990"
     CDS             complement(5793..7499)
                     /locus_tag="PKU75_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF81053.1"
                     /translation="MTETATTGHVGGAGLDLTTLNMMIESLGDFVDEALSPERVLELD
                     HEDECPEDVVRAMSDPNTLGVQLVFIPEAYGGMDGGAFDSYRVCEAMGRKDIGLATAV
                     FATFLGSDPIVVGATEEQKQEWLGAIAEEGVVFAYGATEPEAGSDLGAMTTTADPITG
                     DDGEITAYKINGRKQWISNGSIADFTTILAATPEGPTWFVVPKDTPGFSAGPPEDKHG
                     LRLSNTAALFLDDVEVPAHNLIGTEAGKGLVQAQQVFGYTRLMVAAFGLGGGWEALDR
                     AIAYSKERVQGGSALSEKQGFTHKLIVPHAVRLEAARAFMEDTADKIDRGLGENGAMN
                     TEGAIAKYLATEAGNAAADAAIQAHGGYGYTRPYVVEKIKRDVRITTIYEGTSEIMEM
                     TIARDRWQLHLKSMGRRYTDLATELEQLNNSKPDVGAGIAALGARAMAAVLEACKVGR
                     LTRSQHVLFRLGELIAYCEGASEFAKAAAAAQDGARHEKNPGRFDGAATSAMARVYAR
                     EAAQRIALDGARYVAGAADPGSPALATLAGVPTDAIRAAQAGMLTDMDYIADVLYGRL
                     DG"
     gene            complement(7549..9789)
                     /locus_tag="PKU75_08995"
     CDS             complement(7549..9789)
                     /locus_tag="PKU75_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPF81054.1"
                     /translation="MYWIRLVLGVLMTVVALGMAGRRLGYLYRVGKAGQPIEPGRRSS
                     TGEMVKAEAVEVAAQRKLLRWTGPGLAHAAVFWGFCVLLLTVLEAFGALFDEKFQLPV
                     VGNWPILGFFEDLFALLCIAAVIAFTIIRIKNSPKEEGRKSRFFGSHLGPAWFVLFMI
                     FNVVWTVLFTRGAQINAQNVGGFSLSAADNPDGPLKFLRGAFASQWIAQLFEPLGHRA
                     NEILETVFLLLGLAVLLGFTVFVTYSKHLHILASLPNVAYARRPRALGAALPVYSNGK
                     EVDFEDPGEDDLMGVGKIEDFTWKGLLDFATCTECGRCQSQCPAWNTGKPLSPKVLMM
                     GLRDHALAKAPYLTASEEARESLPDDVKALAEVPLVGATGYAEDNPLGAYTATGPDAV
                     IDSDVIWSCTTCGACVEQCPVDIEHVDHILDMRRHQMLIESAFPEEASVMLNNLEHSA
                     DPWGRGASKRLDWAEGLDFEVRVFGMAGEDTIPDDVDYLFWVGCAGALDETAQRTTRA
                     VATLLHEAGVEFMVLGDGESCTGDPARRIGHEFLFQMLAKQNVEILNEAGAKRIVVTC
                     AHCFNTIANEYPQVGGTYDVIHHSTLLAQLVKDKRLTPVNRVEQSVTYHDPCYLGRHN
                     RIFTPPREVLLGIPGMRVQEMERTRERSFCCGAGGARMWMDENLGSRINLNRTDEALA
                     QQTDVVAAACPFCITMLGDGIAQRQAEGLTRQVEVTDIAELLLRSVRPDQAPEPAEPA
                     GATPVS"
     gene            complement(9827..10789)
                     /locus_tag="PKU75_09000"
     CDS             complement(9827..10789)
                     /locus_tag="PKU75_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPF81055.1"
                     /translation="MSTVLVLAELTPSGVRPATLELLTAARALGEPAAVVVGPVADDV
                     RAALAEHGATTVYAVDAPDADRYLALPKAQALVDLAGQTSPAAILITSGTEGKDVAAK
                     VAVRLDAGIITDAVAVASDGGTVTATQAVVAGSQHATSEFTRTPAVITMRPNATSAEA
                     APAAGEVQQMSVSTADAAQGGEITARTEKAASGRPELTDAAVVVAGGRGVGSEDGFGV
                     IEQLADALGGAVGATRAVTDLSWASHDLQVGQTGKTVAPNLYVAAGVSGAIQHRAGMQ
                     SSKTIVAINKDPKAPIFAISDFGVVGDLHQVLPALIDQVKSRRG"
     gene            complement(10819..11598)
                     /locus_tag="PKU75_09005"
     CDS             complement(10819..11598)
                     /locus_tag="PKU75_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPF81056.1"
                     /translation="MKIVVLVKYVPEPTAVWKFAPDHTLDRAAVDGRLSELDEYAVEQ
                     AVRLVEGGADAEITYVTVGPAKAKDGLLKALAIGGDKGVHIVDDAMHGSDALATSLVL
                     AKALEKVGYDLVLTGMASTDAEMSVVPAMVADRLGINQATFAKSLAVDGDAVTITRDS
                     EAAGLEVKTQLPAIVSVTDQTGEARYPAFRQIMMAKKKPIETLSLADLGVGADSVGSG
                     AAAVKVRSMEPAPARQAGTLITDDGTGAAQVADFLAARGLL"
     gene            11886..12596
                     /locus_tag="PKU75_09010"
     CDS             11886..12596
                     /locus_tag="PKU75_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81057.1"
                     /translation="MGFLDKIFGRTSTSRANQPTSYGPQSYDARPGQSYGTGAPGPQD
                     GIGHTGATEDERAIARYRYLLRTAPPEKLEEVHAHAFGQLSPAQRQQVLGRLRDDLPE
                     GEAPHSDEPREMARAATRAELMRPGYMYSAFGPMSMGGMMMGSLFSTIAGVVIGSAIA
                     DALFDGYDSSPEAAEAGEYDASADPGADAGSDAGSDAGSNAGTDAGSDAGYDAGGGDF
                     GTGDFGGGDFGGGDFGGF"
     gene            complement(13011..>13208)
                     /locus_tag="PKU75_09015"
     CDS             complement(13011..>13208)
                     /locus_tag="PKU75_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81058.1"
                     /translation="DKIAAAANDAYRERWLIEKSGGKLAGPAEQVSLDDDALSIIEIL
                     GEEDALPTPELDAATPSDGDT"
BASE COUNT         1876 a         4287 c         4981 g         2064 t
ORIGIN      
        1 catctcggcc acgatctcgg tcaccttggc cagcacctcg tcaccggccg gagcaccggc
       61 tggtgccggc gccgccggcg cgggggcagc cgcagcagcc ggggcagcag ctgccggcgc
      121 ggcggcagcg gcagacggcc cgggcaggcc gagcttgccg cggacccagc cggcgacatg
      181 ccgcagggtg gggaagtcgc gcaacttgag gttctcgtca cgctccaggt cccacgaacc
      241 acgcacggcc gcaaagacct ccgcctgctt gacggtatcg acgccgaggt cggcctcgag
      301 gtccaggtcg gggtcgagga ggtccgacgg gtaacccgtc atctccgcga cgatgcccgt
      361 gacccggtcg aggatctcgt ccgcaggcgc cgcggcgggc gccgcgggcg ctgccggaac
      421 ggcggcgacc ggggaagcgg ccggagcagc ggcagacggc tcgggcaggc cgagcttgct
      481 gcggacccag ccggcgacat gccgcagggt ggggaagtcg cgcagcttga ggttctcgtc
      541 acgctccagg tcccacgaac cacgcacggc ggcgaagact tccgcctgct tgacggtgtc
      601 gacgccgagg tcggcctcga ggtcgaggtc gggatcgagg aggtccggcg ggtagccggt
      661 catctccgac acgattccgg tgacccgctc caggatctcg tcagccggcg gtgccgcggg
      721 agccgccggt gcggccggcg ccggagcggg ggcagcaggc gtggaggcag caggcgcggg
      781 cgctgcaggt gcaggagccg gggcagcggg cgccgcgggg gcggacggca tactcaccgg
      841 cgccggagcg gccggggcgg ctgctggcgc tgccgcgccg gcgcgggcgc ccagcggggc
      901 caggcgaccg gcatacggca cgggaacccg ggtggtcagg gtgaccggct tggccggggc
      961 cccgttgtcg acgacgcgca ggcgccggtg gtcgacctcc agcgcgctgc cggtgtgccc
     1021 ggacagcgtg tccagccacg tctgccaggc ggcctggtcg atgacgcggt aggcgtagcc
     1081 gagctcctgc ggcgcgcggc gtcggccgtc ggccgggggc acccacttga tcagcgacat
     1141 cgcgatctgc gagccgaacc cggcggccag gcgcagggcg tactcgacgg ggtaggtgcc
     1201 accgcgggag aggttcagcg cgccgaggtc ggggtcgggt tccttgtagt tcggcaccgg
     1261 cgggacgatc ccggtctcga gcgccttgag cgcgacgaca tcctcgatgc cggcgcccat
     1321 ggcgtgcccg gtgaagccct tggtgttggt gatgacgatc cggtcggcgg aggcgccgaa
     1381 gacctgtcgc agcgcgttga tctcggccga ggcgctgccg ccgcgggcgg gcgtataggt
     1441 ctcgtgggag acgaacatcg tcgccggggc gatggcgtgc cggtcgatgc cgcgttgctc
     1501 ggcctcggcg atcagggact gcatgacgcc gccgatgtga tcgacgtcga gacgggtccc
     1561 gtggaaggcg ctgttcgcgg tgatggaggc gagcacctgg gcgatggggg ccaggccgcg
     1621 ctcgcgggcc gactcggccg actcgacgac gatggccgcc gcgccggtgc cgacgatcat
     1681 gccgtggcgg cgcttgtcga acggcgtggc ggcgtcctcg accttctcgt cggtcgcggc
     1741 ggcgcccgtg gcgaggaagc cggtggtgac ccaggggagg aggtgctcgc tggaggcgtc
     1801 gtccgcggag acgacgatga cgcgctcgca ccggccggcc ttgatccagt cctcggccag
     1861 ggagactgcc tgggtcgtgc tggcgcaggc ggcgttgacc tgagtgttcg ggccccgggc
     1921 gccgatgatc tcggcgaact gggagtggcc catcgcgagc acgcggaaca ggaagcggcg
     1981 atcgaacgcg tagggctcgg cctcgagggc ctgacgcagc gcgccgatga gccggtcgac
     2041 ctcggcgcgc gccgggtcgt cggcggtcat gcgggcccgg acgccttcca gcgcaaggag
     2101 attgtcacga cgaccgcgat gcagggcgta cttctcgatc tcctcggcga accggtcgta
     2161 gccggggaag gccgaagcga agatgacgcc cgtggtgtcg cgcagcgcct cgggcagcag
     2221 ccacttgtcg ggcaactgcg tgccgagcgt ggtcttcttg tagcgcatga ccagggggat
     2281 ccccgcgtcg cgcagcgcgt cgaagccggc cgcgaccgcc atccgggtcg tgacgtcgag
     2341 cgcctcgtcg cgcgccttgt ccaggccgaa ctcggccacg acgtcgatcg gcgcgtggat
     2401 gccggcgagc ttgatgacgt cggcgacgtc gtcgatggtc tggaagctgc cgccaccgtg
     2461 agcgtccttg acgatgcggg tgacccgctt gtccgctatg agcgcgcgaa cctcgtgcgg
     2521 cagcacggag atgaagttct caccggcgag gatgcgggcg acattggcgt cgtcgaacat
     2581 cttctcgacg ccgggcaggc cgagcgcggc accggtgatg acgaccggct cgccgcccgt
     2641 cgtgggggcg ccgccggagg cggcggcggg ggccggcaga ccgacgacgg ccgcggcacc
     2701 cttcgagtag ccgaggtcgg cggcatacag ctgcagcccc tggtcgagca cgccggccag
     2761 cagtttgccc agctcggcga tcgtgttgtc ggacaccgtg gttcctccgg tggattgcgg
     2821 gtatgccgtg ggggcggccg gtgcagtcgg cgcggccggt gccgccgcag gagccgcggc
     2881 gggcgccgcc gccggggtcg gcggggcggc gacgggtgcc gccgccgggg cgggctcagg
     2941 gaatccgacg cccgcggcat acaacccgca cagggcctgg ttgaccgcgg tggcgtccga
     3001 gagcttcggg tggttggtga agagtgcggt gacgtcgtcg tgcttgctgc cgaggacgtc
     3061 ctcgacgaag ccgtgcaagg ccttcttggg gccgacttcg acgaagacgc gcgcaccggc
     3121 ggcatacagg gtttccaggc ccttgacgaa ctggaccggc gaggcgacct gctggccgag
     3181 gatctcgagc atgacgtcgg tgctcgcgcc gagcggatag aactcacccg tgacattggc
     3241 gacgcagggc accgtcgggc cgacgacgtt gaggcggcgc agcgcgtcct tgagcggccc
     3301 ggacgccggc gcgacgatcg cggtgtggaa ggcgtggctg acggggatgc gggtggccga
     3361 catccccgcg gccttcgcct tctcgaccag atcctcgacc gcggtggtgg caccgccgac
     3421 gacggcctgg ttgtagctgt tgatattggc gacgacggca tagccgtccg cggtcttgac
     3481 taactcttcg atcgcggtga gcgggccgaa catcgcggcc atcgcgccgt tgtcggcgac
     3541 cgagaccttc ttcatctcgt tgccgcgggc gctgacggct tccagggcgc tctcgagcga
     3601 gagggagccg gcggcgacga gggcgccgta ctcgccgagg ctgtggccca tcaccatgtc
     3661 ggggcgcacg ccgtatgccg cgagcaggcg cgtcagcgac aggtcggtcg cgagcacggc
     3721 cggctgggtg atctcggtct gcatcagctg cttgttgagc tggtcgaccg cgtccttgtc
     3781 gtcgccgtcg atgaagatgt acgacgtcag ggggcgaccc ccgaggagcg gggtcatcac
     3841 ccggtccgcc tcgtcgaagg tcgccttgac gaccggctcg ttggccgcga ggtccttgag
     3901 catgttgacg tattgcgagc cctggccggt gtagaggaag gcgaccttgg gtgcggggcc
     3961 gcggccgacg aaaacgcctt gctggcgaag gagtttgaag gcggccggcg cgccggaggc
     4021 catggccttg accagcttgt ccagcttgcc ggcgagctcg gcggcgtcgc cgtagtcgac
     4081 ggcgacgcgc accgccgcat ccttgaccga acggtcgggc gcggccggtg cgggcgcggt
     4141 gccggcctgg gcggcggcaa gggcttcgcg gaccttgccg agcacttcgg catcgtcgcg
     4201 accgccgagg acgagggcac cccgcagggg cgccttggcg ggtccgccgg cggcaccggg
     4261 gccggcgaca ggcgcggaaa ccgttgctgc ggaaggcact tcagcggagg cgaagacctt
     4321 ggccggagcc ttgtgtcggc cgggaatgtg ctcctccaac accacgtgga agttggtgcc
     4381 gccgaagccg aaagcgctca cggcgccgcg ccgcacaccg tgcgggctgt cgggccaggc
     4441 gcgcaggctg gtgttgacct tgaatgggat cgagccccag tcgacgttct cgttggggtt
     4501 gttgaagttc aggctcgggg cgaggacctt ctcgtgcaac gagcggacca tcttgaacag
     4561 gcccgcggtg ccggcggccg ccttgaggtg gccgatgttc gacttaaccg agccgagggc
     4621 aaccgatccg ggtgccaggt tcgaggagcc gaaaacctct gtgagcgagg cgagttcggc
     4681 ggcgtcgccg acgcgtgtcg acgtgccgtg ggcctcgacg gccatcacgg tggcggggtc
     4741 gagaccggcc tgctcccagg ctcggcgcac ggcgagttgc tggccgatcg ggttgggcgc
     4801 ggtgatgccc ttgcccttgc cgtcggagct gccgccgacc gacatgatca ccgagtagat
     4861 ccggtcgccg tcgcgctcgg cgtcctccag acgcttgagc acgaacaggg cggctccctc
     4921 gcccatgacg aagccgtcgg cgccggagtc gaacggccgg gtgccagtgg cggacagggc
     4981 gccgatcttg cagaacttca cgaaggcgtc gatgcccatg ttgcggtcga cgccaccggt
     5041 gacgacggca tcgtattggt gatcgaccag gccgaggatg gccgagttca gggccgcaag
     5101 tccggaggcg caggccgcgt cggtggtgaa gttggggccg cgcaggttga gcaactggca
     5161 gacccggccg gcgatgacat tcgccagctc accgggcatg gtgtcctcgg tgatctcgaa
     5221 ggtgttgtcg aggtagcccg cacgcgtctc ctcgacgatg cgctgctgga cgtcggcggg
     5281 caatccctgg agagtggccg actcctgcag ccgcttgatc acctccggca ggcgcacgcg
     5341 catcgaggac aggtagtgct tctcgccacc gatggcgttg ccgaggatga cggcgacatt
     5401 gttgctgtcg acattccact ccggccagcc ggcgtcgatc agcgccgcgc gggcggcgct
     5461 gatcgcccag cgctggccct cgtccatctg agcagccacg gtcggcggca cgggcaggcg
     5521 ccacttgatc gggtcccagt cgaactcgcg cacccagcca ccgatggtgc tgtaggtctt
     5581 gtcgcgcgct ccgtggtccg ggctgaagta gagcgcggga tcccagcgct ccgggggtac
     5641 ctcagtgacg ctgtagcgcc cgcctttgat gttgtcccag aaggcattcg ccgacggcgc
     5701 ctgcggcatg atcgccgcca tcccgacaac ggccaccggc tttggtgtgt tggtcatcct
     5761 cgtccactcc ctgcgtacag atatctcgaa agtcagccgt cgagtcgtcc gtagaggacg
     5821 tcggcgatgt agtccatgtc ggtgagcatc ccggcctgcg cggcccggat cgcgtcggtg
     5881 ggcactcctg ccagcgtggc cagggcggga gagccggggt cggccgcacc cgcgacatac
     5941 cgcgccccgt cgagcgcgat gcgctgggcg gcctcacggg catacacccg cgccatggcg
     6001 gaggtggcgg cgccgtcgaa gcggccgggg ttcttctcgt gccgggctcc gtcctgcgcc
     6061 gcggcagcgg ccttggcgaa ctcggacgcg ccctcgcagt aggcgatcag ctcgccgagc
     6121 cggaagagca cgtgctggct gcgggtcagg cgcccgacct tgcacgcctc gaggaccgcg
     6181 gccatcgcgc gggcgccgag ggcggcgatg ccggcgccga cgtcgggctt gctgttgttc
     6241 agctgctcga gttcggtcgc gaggtcggtg taacgacggc ccatcgactt caggtgcaac
     6301 tgccaccggt cgcgggcgat cgtcatctcc atgatctccg aggtgccctc gtagatggtc
     6361 gtgatgcgca cgtcccgctt gatcttctcc acgacatacg gccgggtgta tccgtaaccg
     6421 ccgtgcgcct ggatggcggc atccgcggcc gcgttaccgg cttctgtcgc aaggtatttc
     6481 gcgatggcac cttcggtgtt catggcgccg ttctcgccga ggccgcggtc gatcttgtcg
     6541 gcggtgtcct ccatgaacgc gcgggcggcc tcgagccgga cggcgtgggg gacgatcagc
     6601 ttgtgggtga aaccctgctt ctccgacagg gcgctgccgc cctggacccg ctccttcgaa
     6661 taggcgatgg cccggtcgag ggcctcccag ccgccgccga gaccgaaggc cgcgaccatg
     6721 agccgggtgt agccgaagac ctgctgcgcc tggaccaggc ccttgccggc ctcggtcccg
     6781 atgaggttgt gcgccggcac ctcgacgtcg tcgaggaaga gggcggcggt gttggacagc
     6841 cgcaggccgt gcttgtcctc cggggggccg gcgctgaagc cgggggtgtc cttggggacg
     6901 acgaaccacg tcgggccttc gggggtggcg gcgaggatcg tggtgaagtc ggcgatggag
     6961 ccgttggaga tccactgctt gcggccgttg atcttgtatg ccgtgatctc gccgtcgtcg
     7021 cccgtgatcg ggtcggcggt ggtcgtcatc gcgccgaggt ccgagccggc ctccggctcg
     7081 gtggcgccgt aggcgaagac gacgccctct tcggcgatgg cgccgagcca ctcctgcttc
     7141 tgctcctcgg tggcgccgac gacgatcggg tcggagccga ggaaggtcgc gaacaccgcg
     7201 gtcgcgaggc cgatgtcctt gcgccccatg gcttcacaga cgcggtagct gtcgaacgct
     7261 ccgccgtcca tgccgccgta ggcctcgggg atgaagacca actggacgcc cagggtgttg
     7321 gggtcggaca tcgcccgcac gacgtcctcg gggcactcgt cctcgtggtc gagctcgagg
     7381 actcgttcgg gggagagcgc ctcgtccacg aagtcgccga gcgactcgat catcatgttc
     7441 aaggtggtca ggtccagccc ggctccgccg acatggcccg tggtcgcggt ctcggtcatc
     7501 gttctctcac ttttctcttc ggtcgtgtcg ggctgaggtg gttcgtggtc aggagacggg
     7561 ggtggcaccg gccggttcgg ccggctcggg cgcctggtcc gggcggaccg agcgcagcag
     7621 gagttcggcg atgtcggtga cctcgacctg gcgggtcagg ccttcggcct gccgttgggc
     7681 gatgccgtcg ccgagcatcg tgatgcagaa gggacaggcg gccgcgacca cgtcggtctg
     7741 ctgcgccagt gcctcgtcgg tgcggttgag gttgatgcgg gagccgaggt tctcgtccat
     7801 ccacatccgc gcgccgccgg cgccgcagca gaacgagcgc tcgcgggtgc gctccatctc
     7861 ctggacccgc atgccgggta tgccgagcag gacctcccgc ggcggggtga agatccggtt
     7921 gtgccggccg agatagcacg ggtcgtggta ggtcaccgac tgctcgaccc ggttcaccgg
     7981 ggtcaggcgc ttgtccttga cgagctgggc gagcagcgtc gagtggtgga tgacgtcata
     8041 cgtcccgccg acctgcgggt actcgttggc gatcgtgttg aagcagtgcg cgcaggtgac
     8101 gacgatgcgc ttggcgccgg cctcgttgag gatctcgacg ttctgcttgg cgagcatctg
     8161 gaagaggaac tcgtgcccga tgcggcgagc cgggtcgccc gtgcacgact cgccgtcgcc
     8221 gagcaccatg aactccacgc cggcctcgtg cagcagtgtc gcgacggcgc gggtggtgcg
     8281 ctgcgcggtc tcgtccaggg cgccggcgca gccgacccag aagaggtagt cgacgtcgtc
     8341 ggggatcgtg tcctcgccgg ccatcccgaa gacgcgcacc tcgaagtcga ggccctcggc
     8401 ccagtcgagg cgcttggagg cgccgcggcc ccacgggtcg gccgagtgct cgaggttgtt
     8461 gagcatgacc gaggcctctt cggggaaggc cgactcgatg agcatctggt ggcggcgcat
     8521 gtcgaggatg tggtcgacgt gctcgatgtc gacggggcac tgctcgacgc aggcgccgca
     8581 ggtggtgcac gaccagatga cgtcggagtc gatgaccgcg tccgggccgg tggcggtgta
     8641 ggcccccagg gggttgtcct cggcatagcc cgtcgcgccg acgagtggca cctcggcgag
     8701 cgccttcacg tcatcgggca gcgactcgcg ggcctcctcg gaggcggtga ggtatggcgc
     8761 cttcgccagc gcgtgatcgc gcaggcccat catcaacacc ttgggcgaca acggttttcc
     8821 ggtgttccac gccgggcact ggctctggca gcggccgcac tcggtgcagg tcgcgaagtc
     8881 gagcaggccc ttccatgtga agtcctcgat cttgccgacc cccatcaggt cgtcctcgcc
     8941 cgggtcctcg aagtcgacct ccttgccgtt cgagtagacc ggcagtgccg cgcccagggc
     9001 gcgggggcgg cgggcgtagg cgacattggg cagcgaggcg aggatgtgca ggtgcttgct
     9061 gtaggtcacg aagacggtga acccgagcag cacggccagg ccgaggagga ggaagacggt
     9121 ctcgaggatc tcgttggccc ggtgcccgag cggctcgaac agctgcgcga tccactgcga
     9181 ggcgaaggcc ccgcggagga acttcaacgg gccgtccggg ttgtcggcgg ccgagaggga
     9241 gaagccgccg acgttctggg cgttgatctg ggcgccgcgg gtgaacagca cggtccacac
     9301 gacgttgaag atcatgaaca gcacgaacca cgccgggccg aggtgactgc cgaagaagcg
     9361 gctcttgcgg ccttcctcct tgggagagtt cttgatccgg atgatcgtga aggcgatgac
     9421 cgccgcgatg cacagcaacg cgaagaggtc ctcgaagaag ccgaggatgg gccaattgcc
     9481 gacgaccggc agttggaact tctcgtcgaa gagggcgccg aaggcctcga ggacggtcag
     9541 gagcaggacg cagaatcccc agaagaccgc ggcgtgggcg aggccgggcc cggtccagcg
     9601 caagagcttg cgctgggccg cgacctcgac ggcctcggcc ttgaccattt ccccggtgga
     9661 ggaccggcgg ccgggctcga tgggttgccc ggccttgccg acccggtaga gatagccgag
     9721 gcggcgcccc gccatgccca aggcgacaac ggtcatcagc acgccgagga cgagtcggat
     9781 ccagtacata cgggtcgctc cttcgcgagg gtggtgcgtg ccgggctcag ccgcggcggg
     9841 acttgacctg gtcgatcagc gccggcagca cctggtgcag gtcgccgacg acaccgaagt
     9901 cgctgatcgc gaagatcggc gccttggggt ccttgttgat ggcgacgatg gtcttgctcg
     9961 actgcatacc cgcgcggtgc tggatggcgc ccgaaacacc cgctgcgaca tacagattcg
    10021 gcgcgaccgt cttgccggtc tgaccgacct gcaggtcgtg ggaggcccag ctgaggtcgg
    10081 tgacggcgcg ggtggcgccg acggcgccgc cgagagcgtc ggcgagctgc tcgatgacac
    10141 cgaaaccgtc ttcggacccg acgccgcggc caccggccac gacgaccgcc gcgtcggtga
    10201 gctccggccg gccgctggcg gccttctcgg tgcgcgccgt gatctcgccg ccctgggccg
    10261 catcggcggt ggagaccgac atctgctgca cctcgcccgc tgccggcgcg gcctcggcgg
    10321 acgtggcatt gggccgcatc gtgatgacgg cgggggtgcg ggtgaactcg ctcgtggcgt
    10381 gctgcgagcc ggcgacgacg gcctgtgtcg cggtgacggt gccaccgtcg gaggcgacgg
    10441 cgacggcgtc ggtgatgatg ccggcatcga gccggacggc gaccttcgcc gcgacatcct
    10501 tgccctcggt gcccgaggtg atcaggatcg ctgcgggcga ggtctgcccg gcgaggtcga
    10561 cgagcgcctg ggccttgggc agcgcgaggt aacggtcggc gtcgggggcg tcgacggcat
    10621 acacggtggt ggcgccgtgc tcggcgagcg cggcgcgcac gtcgtcggcg accggcccga
    10681 cgacgaccgc ggcgggttcg ccgagggcgc gggcggcggt gagcagttcg agggtcgcgg
    10741 ggcgcacgcc ggagggggtc agttcggcga gaacgagaac ggtggacacg tcagggttcc
    10801 tgtcttcttc ggcgggggtc agaggaggcc gcgggcggcg aggaagtcgg cgacctgggc
    10861 ggcgcccgtg ccgtcgtcgg tgatgagagt gccggcctgg cgggcaggag cgggctccat
    10921 gctgcggacc ttgaccgcgg cggcgccgga gcccacgctg tccgcgccga cgccgaggtc
    10981 ggcgagcgag agggtctcga tgggcttctt cttcgccatc atgatctggc ggaacgccgg
    11041 gtagcgcgcc tctccggtct ggtcggtcac cgagacgatg gcgggcagct gggtcttgac
    11101 ctcgagcccg gcggcttcgg agtcgcgggt gatcgtcacc gcgtcgccgt ccacggcgag
    11161 ggacttggcg aaggtcgcct ggttgatgcc gagccggtcg gcgaccatcg cgggcacgac
    11221 ggacatctcg gcgtcggtgg aggccatccc ggtcaggacc aggtcgtagc cgaccttctc
    11281 cagcgccttc gcgaggacga gcgaggtggc gagagcgtcg ctgccgtgca tggcgtcgtc
    11341 gacgatgtgc acgcccttgt cgccgccgat ggccagggcc ttgagcagcc cgtccttggc
    11401 cttggcgggg ccgacggtca cgtaggtgat ctcggcgtcc gcgccaccct cgacgaggcg
    11461 cacggcctgt tccacggcat actcgtcgag ctcggacagg cgcccgtcga cggcggcccg
    11521 gtcgagggtg tggtcgggag cgaacttcca cacagcggtc ggctcgggga cgtatttgac
    11581 gaggaccacg atcttcatgg acaacctgcc ttcgcaagag gtgcgtgtcg tattaggcgg
    11641 cccggcggcg ctgccgagcg ccagtagttg tgaacctact cctgaggggt agtgcacgga
    11701 aaccggaaca ccaggcgaga tgcccaaagt cccccaggac ggcctccgga gcggggtttt
    11761 gccgccctcg cgcagagctt ccgcggcgaa atcccaactg ccagaacgct cccaggtcgc
    11821 gtcgcggccc taagatgacc tccatcggcc ccgtccgagg ggtggacctg aggggtggtt
    11881 tgaacatggg tttcctggac aagattttcg gccggacgtc cacatcgcga gcgaatcagc
    11941 ccacgtcata cggcccgcag tcgtatgacg cgcggccagg ccagtcgtac ggcacgggcg
    12001 ctcccggtcc gcaggacggc atcgggcaca ccggcgcgac cgaggacgaa cgcgccatcg
    12061 cccgctatcg ctatctgttg cgcaccgcgc cgcccgagaa gctggaagag gtgcatgcgc
    12121 acgcgttcgg tcagttgtcc ccggcgcagc gccagcaggt gctcggccgg ctgcgggacg
    12181 acctgcccga gggggaagct ccgcacagcg acgaaccacg cgagatggcc cgcgccgcca
    12241 cccgcgccga gctgatgcgg cctggctata tgtattcggc gttcgggccg atgagtatgg
    12301 gcgggatgat gatgggctcc ctcttctcca ccatcgccgg tgtcgtcatc ggctcggcca
    12361 tcgccgacgc cctcttcgac ggctacgact cctcgcccga ggccgccgag gctggggagt
    12421 atgacgcctc cgccgacccc ggcgcggatg ccggctccga tgcgggctcg gacgccggtt
    12481 cgaacgccgg taccgatgcg ggatcggacg ccgggtatga cgcgggaggc ggcgacttcg
    12541 gcaccggcga cttcgggggc ggggacttcg gcggcggcga cttcggcggc ttctgagcgc
    12601 gccagcgcgg tgtggagccg atccgtgcac accggctacg agtgccgcgg cctgcgaact
    12661 cgggcaagcc tgtgacctgc aggtatgcca gccgcgccga cgagcaggtg cactactcgt
    12721 agctggtgtc tgccgcggag ggcagcagag gtaacgcgaa ggggccgcac ccggcgtggg
    12781 tgcggccccg gcgtcgtcca gcgagtcgcg tcgtccaatg agtcggcgtc gctcagcaac
    12841 tcagaggtcg aagtacatct cgaactcccc agccggtgtt gggcggggtt ggagatctcg
    12901 ggaaaacacc gtctgacctg tagaaacgct gtttcggcag gttgacccgt gacggctgat
    12961 ttcggcctcg aacggttctc ctgcggtatc actgcggtat ttggagggcg tcacgtgtct
    13021 ccgtctgaag gggtcgcggc atccaactcc ggagtcggga gcgcatcctc ttcgccgagg
    13081 atctcgatga tgctgagggc gtcgtcgtcg agcgaaacct gttccgcggg gcctgccagt
    13141 ttgccgcccg acttctctat gagccaacgc tcacggtagg cgtcgttggc ggccgcggcg
    13201 atcttgtc
//
LOCUS       DAOPJG010000084        12997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5043_length_12997_cov_6.782723, whole genome shotgun sequence.
ACCESSION   DAOPJG010000084 DAOPJG010000000
VERSION     DAOPJG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5043_length_12997_cov_6.782723"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..905
                     /locus_tag="PKU75_09020"
                     /pseudo
     CDS             179..905
                     /locus_tag="PKU75_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            942..1787
                     /locus_tag="PKU75_09025"
     CDS             942..1787
                     /locus_tag="PKU75_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPF81059.1"
                     /translation="MKTRRIGALIGATTLALALGACGSSDDGTASGGSSGSAADGDSI
                     KIGIKFDQPGLGLFDAGKYSGFDVDVANYVAKELGKTNVTFIQSPSKQREDLIATGQV
                     DLIFATYSITDERKQKVSFAGPYFVAGQDLLVATDSDITGPEGLDGKKLCSVTSSTSA
                     QKVKDSYPGVQLQNFDTYSKCLDLVVNGQLDALTTDNTILAGYAAQPAYKGKLKVVGK
                     TFSEERYGVGLKKGDVALCEKVNTALKKMVDSGEWQKAVDANFGPAGFKVDTATNPPK
                     TDACS"
     gene            1891..2571
                     /locus_tag="PKU75_09030"
     CDS             1891..2571
                     /locus_tag="PKU75_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPF81060.1"
                     /translation="MDELFDQFDIWSAFGQTIYLAFFSALGALILGTVLAVMRVGPVG
                     IFNRAGAAYVNLVRNTPLTLIVTFCLLALHYQLKINLAPATSSTSIVDNGVRWAIVGL
                     IVYHAAFVCEALRSGINTVPPGQAEAARSIGLGFGQSLRYVVLPQAFRGAIAPLASVL
                     IALIKNTTVAYAIGVAETAAFMRDALEFRPDLSLMIFLLVAGFFVLLTMPLGILLTNL
                     SQRMAVKR"
     gene            2568..3512
                     /locus_tag="PKU75_09035"
     CDS             2568..3512
                     /locus_tag="PKU75_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPF81061.1"
                     /translation="MSTSVLFDAPGPKARRRHRVVAITGGLLLLVLLALVGRALHANG
                     EFAGAKWSPFADWGTWRYYLLLGIWNTIKAAAAAVVLAMIFGFVFGVGRLSRNVVIRW
                     VSGAIVEFFRAVPVLIMMIFFFGVFAYNQVFPAAWNPFAAVVTALTLYNGSVIAELVR
                     SGVFGLPKGQAEAGLSIGLTPGQTLRNIQLPQAITAMLPALVSQLVVILKDTALGTII
                     TYPELLQQAQNLASARSNVIPALLVAAAVFIAINYLLTWLAARLEARINRRGHTAGAA
                     IGRAPTVPGAPVDQAHAAALTPHEARHDAAHAGQQDRV"
     gene            complement(3521..3823)
                     /locus_tag="PKU75_09040"
     CDS             complement(3521..3823)
                     /locus_tag="PKU75_09040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPF81062.1"
                     /translation="MHPIWLARLDKTRPVLVLTREEVRAVRQLITVAPITTTVRGLSS
                     EVTLGPHNGLEHPCVANLDSIVTVPRRALIRPLGALLEEQERDLTRAFHEAFDLAD"
     gene            complement(3823..4068)
                     /locus_tag="PKU75_09045"
     CDS             complement(3823..4068)
                     /locus_tag="PKU75_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HPF81063.1"
                     /translation="MGVQMTIRVDDDLAAFVDAQAAARTTSRADVINRAIRGEIRRQR
                     AARDGQIYASTTDPDLESDAYAAWAAGTAGSAWSELD"
     gene            complement(4126..4749)
                     /locus_tag="PKU75_09050"
     CDS             complement(4126..4749)
                     /locus_tag="PKU75_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81064.1"
                     /translation="MLIANYLRPLPRIGVDVVTLAAMIPDRRDAAEALHTCPSGWLGY
                     ELRTFPRPRRDLPAWLAAARADGADGHHRLIHKVDTWFDRHLVSQWAGARDAPDAVYD
                     RLSREFTGGGVDALVVASGALHPMGRPRTAHPRRRPVVAGVARDAAPEALRDASSRPS
                     GAFADPRPDGVFWSLMRDAAGQLYVGDMGGRAPVPGGGRIYAVVAAD"
     gene            5000..6292
                     /locus_tag="PKU75_09055"
     CDS             5000..6292
                     /locus_tag="PKU75_09055"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPF81065.1"
                     /translation="MSSHLDRRSALRWGGGALLLAGLAGCTATAATDSGDASSNGSGA
                     TTAAGGAASARPAASGAGVFDSATLHTLDIAMSEADFAGVLAAYQADQSKEWVEASVT
                     IDGTSYERCGVRLKGNSTIWRVADGASASEYPWLVRLDKYVDGQAHDGVTEIVVRLNN
                     TASALNEALSLDLLAKAGLASQPWSYAAVSAAGATPVLRLILEHPSDPWVAREFGEDG
                     VLYKAEATSNYDYLGDEPSLYDERFDVEAGDDDLAPLIEFLKWVNEIDDAGFAADLST
                     HVDVAAFASYLALEDLIGNYDAIDGPGNNSYLWWAKAADLMTVVGWDHNLTFGVTNRP
                     GGQGGGAPGGPGGMPAGGANRGNQSGNVLARRFEADATFAAQKSAATERLRAELYTSG
                     YAAQRLSEIAAMLTEVAGHNIGAETITSEKAAIATYLA"
     gene            complement(6379..7929)
                     /locus_tag="PKU75_09060"
     CDS             complement(6379..7929)
                     /locus_tag="PKU75_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF81066.1"
                     /translation="MPVTEAEPEYPTLDPDDLETTLRVLAGLHELAPDDPDIQTVKRA
                     TGVMASRIRKARRREARQAALAPGRERDKAVLESTATGSPLRIDDETRGIPLVAQADS
                     PYAGELETPRGCYICHEKYTLVDAFYHWLCPRCATFSHGKRDQHTDLRGRRALLTGGR
                     AKIGMYIALMLLRDGADLTITTRFPRDAARRFAELSDSAEWLDRLTIVGIDLRDATQV
                     VALADEVAAGGPLDILINNACQTVRRTPGAYAHLVAGEGEPLPAGMPLPRLIAFDRIS
                     EAHPASLVGVLAEHAHTHPDGEGTDGEGTDGGGPDGGGAVSAASLTSVALQAGSASLD
                     RHVAGTAIDAGGLLPDDVLVNSWTQKVEDVDPLELLEVQLCNSVAPFLLVSRLRPSMR
                     AAVERGARRAYVVNVSAMEGQFSRRYKGPGHPHTNMAKAALNMLTRTSAKEMFETDKI
                     LMTAVDTGWITDERPHREKLRIAAEGWHAPLDLVDGAARVYDPIVRGEAGEDLFGVFL
                     KDFAPHPW"
     gene            7993..8994
                     /locus_tag="PKU75_09065"
     CDS             7993..8994
                     /locus_tag="PKU75_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81067.1"
                     /translation="MRTAPLAIASALVLLLTACGTQVADPGASGSGGAAPTTGTGAAG
                     MPPGATATPAGGGSSSAAKSGPPSAKATSGTGEFIAAATVLEKKGEGPQLCLGGVAES
                     LPPQCGGPVIDNWDWATAPKSETAAGVRWGEYLVIGTYDAAASTFHLTKPATTREEYD
                     GPLPTAPPDAMFTTPCPVPEGGWRVVDPAKATRAAQDAALEAAAKLPTYAAAWLDQSI
                     NPADPEKQPEAMNDPVKLILNVKVTTDAAGAEKQLREIWGGMLCVSEGGHPEAELRKI
                     QEELSGKHGMLFSAVDGQTSTVEFGVIWDDGSLQKAMDAAYGLGVVHVTSALQPYPG"
     gene            9152..11023
                     /locus_tag="PKU75_09070"
     CDS             9152..11023
                     /locus_tag="PKU75_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPF81068.1"
                     /translation="MGHYKANLRDIEFNLFEVFGRDEVFGHGPYAEVDTDTAKEMLKE
                     VARLAENELAASFVEADRNPPVFDATTNSVTMPESFAKSYKAYIDSGFWNIDVPGELD
                     GTVAPPSMKWAMNEMILGANPAIAMFAASYSFGKLLYILGTDEQKKMAKWIIEKGWHC
                     TMVLTEPDAGSDVGAGRTKAVQNEDGTWNVSGVKRFITSGESDMTDNVVHFVLARPEG
                     AGPGTKGLSLFIVPKFHVDLETGELGERNGVYVTNVEDKMGLKVSTTCEVTFGDKHPA
                     IGTLFGDVHDGIAQMFRVIEHARMLVGTKAIATLSTGYLNALEYAKNRVQGADLTQMA
                     DKAAPRVTITHHPDVRRSLMLQKAYAEGLRSLVLFTACQQDIVDQAQLESGSEQLEKD
                     SPADLASRVNDLLLPIVKGLGSERAWVLLGTESLQTFGGSGFLKEYPIEQYVRDAKID
                     TLYEGTTAIQGLDFFFRKIIRDKAQALSTVSMQIQEFAKDLGQQGGKLDKDRELLSKG
                     VEDVQGIVTAMVGQLMSGDPRTGGDVTNVYKVGQNTSRVLLAAGDLIVGWLLLRQAAI
                     AQEKLDAGATGADKDFYTGKVAAAQFFSRNVLPKLAAERAIAEAMDNSLMEVPESAF"
     gene            complement(11088..12026)
                     /locus_tag="PKU75_09075"
     CDS             complement(11088..12026)
                     /locus_tag="PKU75_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPF81069.1"
                     /translation="MSIHEVFTTPGPVALEVDLDHGQVVVVAAAQATTSVDVTGPHAD
                     DFSVEHRGDGVRVAPPKWRGWGFGRHTVRIGVPDGSSVRARCGSAGIDLEGELGEVTL
                     KCGSGGVHCVRAADLMFESGSGTIEIGHVEKLQGKSGSGSVHIGRLTGEGQVATGSGS
                     IHLDRVVGSLDAKSGSGKLRITSASGDIRCRNGSGAIGIDVIWRGRADLRTTSGRVRL
                     GIEEGKPVWADVSSVSGTVRSTLPPVGEPGPGQDHVELHVHTVSASISLDPAPKGLQD
                     DTEPARPYDAPRGPDRAAAYAPIPTEQAVELSAQDS"
     gene            complement(12038..12598)
                     /locus_tag="PKU75_09080"
     CDS             complement(12038..12598)
                     /locus_tag="PKU75_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81070.1"
                     /translation="MEMSPYVERLREALDSAAAGAGADVQDAADRLNRALDAAVRLTL
                     FEVLADAAAEITSSLPSGSVTARLSGRGVDFVVEGVARGETLPLVSDGSLSTQPPGSG
                     GSIQDAALDEGDLARITVRLPEGIKDRAEEQAGRLGQSLNSWIVKTLRQATGPTGIQL
                     DLSGDRSFADQDFPHGGSRGRLSGWI"
     gene            12758..>12997
                     /locus_tag="PKU75_09085"
     CDS             12758..>12997
                     /locus_tag="PKU75_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HPF81071.1"
                     /translation="MWVGLGIFLLVVGAILSFAVRDSIDAVDLTMIGYILMGGGALAV
                     LLSLIMGSRRTAAGGYESRTVSHVDPNTGTRVEEHR"
BASE COUNT         2131 a         4477 c         4424 g         1965 t
ORIGIN      
        1 tagagcgcca gcttcccaag ctggacacgc gggttcgatt cccgtcaccg gctctctggc
       61 tgccagcagg gccgggcggg gcaccggcac tgccaaggtt gcaatccggc gacatccacc
      121 cacggcgggc ggggcgggtc ccgtgggccg ttgtaggttc ggccgcatgg cccagcccct
      181 ggtcgtcctc caggacgtca acaagcactt cggagacctc catgtgctca agagcatcga
      241 cctgagcgtc gacaagggcg aggtcgtcat cgtcatcggc ccgtccggct ccggcaagtc
      301 gacgatgtgc cgcacgatca accggctcga gacgatcgag agcggcacca tcacgatcga
      361 cggcaagcca ctgcctgagg agggcaagga cctcgcccag ctgcgcgcgg acgtcggcat
      421 ggtcttccag tccttcaacc tgttcgcaca caagacgatc ctcgacaatg tcacgctcgg
      481 cccgatcaag gtccgcaaga aatccaagtc gcaggcagag aaggacgcca tggcgctgct
      541 ggagcgggtc ggtgtccagg agcaggcgca caagtacccg gcccagctct ccgggggtca
      601 gcagcagcgc gtggcgatcg cccgcagcct cgcgatggaa cccaaggtca tgctcttcga
      661 cgagcccacc tcggccctgg accccgatga tcaacgaggt cctcgacgtc atggtcggcc
      721 tcgccaagga cgggatgacg atgatcgtca tcacccacga gatgggcttt gcccgcaagg
      781 cggccaatcg cgtcgtcttc atggacgacg gccagatcgt cgaacaggcc accccgcagg
      841 agttcttcac caatccgcgt tcggcgcggg ccaaggactt cctcgccaag atcctcaccc
      901 actagaaccg acaaccgcgc accaccggaa ggaacacaac catgaagaca cgcaggatcg
      961 gcgccctcat cggagcgacc accctggccc tcgcactcgg tgcctgcggc agcagtgacg
     1021 acgggaccgc gagcggcggc tccagcggca gcgccgccga cggcgacagc atcaagatcg
     1081 gcatcaagtt cgaccagccc gggctcgggc tcttcgacgc cggcaagtac tccggcttcg
     1141 acgtcgacgt cgcgaactat gtcgccaagg aactcggcaa gaccaatgtg accttcatcc
     1201 agtcgccgag caagcagcgc gaagacctga tcgccaccgg ccaggtcgac ctgatcttcg
     1261 cgacctactc gatcaccgac gagcgcaagc agaaggtctc cttcgccggg ccgtacttcg
     1321 tcgccggcca ggacctcctc gtcgcgaccg acagcgacat caccgggccg gagggcctcg
     1381 acggcaagaa gctgtgctcg gtgaccagct cgacctcggc ccagaaggtc aaggacagct
     1441 accccggcgt ccagctgcag aacttcgaca cctacagcaa gtgcctcgac ctggtcgtca
     1501 acggccagct cgacgcgctc accaccgaca acacgatcct cgccgggtat gccgcccagc
     1561 cggcctacaa gggcaagctg aaggtcgtcg gcaagacctt ctccgaggag cgctacggcg
     1621 tcggcctcaa gaagggcgac gtcgccttgt gcgagaaggt caacacggcg ctgaagaaga
     1681 tggtcgactc cggcgaatgg cagaaggccg tcgacgcgaa cttcggcccg gccgggttca
     1741 aggtcgacac cgcgaccaac ccgccgaaga cggacgcctg ctcctgacct gacccctccg
     1801 gaacccgagc aggggtatgc cgccgccgcg cggcataccc ctgcccaccg gaccacggca
     1861 ggaccggacc cgacacggaa gacgtgagcc atggatgaac tgttcgacca gttcgacatc
     1921 tggtccgcct tcggccagac gatctacctc gccttcttct ccgcgctcgg cgcgctgatt
     1981 ctcgggaccg tcctcgcggt gatgcgggta ggcccggtgg ggatcttcaa ccgggccggc
     2041 gcggcatacg tcaatctggt gcgcaacacg ccgctgacgc tgatcgtgac attctgcctg
     2101 ctggctctgc actaccagtt gaagatcaac ctggcaccgg cgacgagttc gacgtcgatc
     2161 gtcgacaacg gggtgcggtg ggcgatcgtc ggcttgatcg tctaccacgc ggcgttcgtg
     2221 tgtgaggcgc tgcgcagcgg catcaacacg gtgcctcccg ggcaggccga ggcggcccgg
     2281 tcgatcgggc tcggcttcgg tcaaagcctg cggtatgtcg ttctgccgca agcgttccgc
     2341 ggcgccatcg ccccgctggc gtcggtgctc atcgcgttga tcaagaacac aactgtcgcg
     2401 tatgcgatcg gggtcgccga gacggccgcg ttcatgcggg acgcgctgga gttccggcct
     2461 gacctgagcc tgatgatctt cctgctcgtc gccggcttct tcgtcctgct gacgatgccg
     2521 ctcggcatcc tgctcaccaa cctctcgcag cggatggcgg tcaagcgatg agcacctcgg
     2581 tcctcttcga cgcccccggc cccaaggccc ggcgccggca ccgcgtggtc gccatcaccg
     2641 gcgggctgct gctgttggtc ctgctggcgc tcgtggggcg tgcgctgcac gccaacgggg
     2701 agttcgccgg ggcgaagtgg agccccttcg ccgactgggg aacctggcgc tactacctgc
     2761 tgctggggat ctggaacacc atcaaggccg ccgcggccgc ggtcgtcctg gcgatgatct
     2821 tcgggttcgt cttcggggtg ggccggctct cgcgcaatgt cgtcatccgc tgggtgagcg
     2881 gggcgatcgt cgagttcttc cgcgcggtcc cggtgctgat catgatgatc ttcttcttcg
     2941 gggtgttcgc gtacaaccag gtcttcccgg ccgcgtggaa cccgttcgcc gcggtggtga
     3001 cggcgctgac gctctacaac ggctcggtga tcgcggagct ggtgcggtcc ggggtcttcg
     3061 ggctgccgaa gggccaggcg gaggccggcc tctcgatcgg gttgactcca ggccagacac
     3121 tgcgcaatat ccaactgccg caagcgatca cggcgatgtt gccggcgctg gtcagccaac
     3181 tcgtcgtcat cctcaaggac accgccctcg ggacgatcat cacctatccg gagctactgc
     3241 agcaggcgca gaacctcgcg tcggcccgct cgaatgtcat ccccgctttg ctcgtcgccg
     3301 cggccgtctt catcgcgatc aactacctgc tgacctggct ggcggctcgg ctcgaggccc
     3361 ggatcaaccg gcgcgggcat acggccggtg ccgccatcgg ccgggcgccg acggtgccgg
     3421 gcgcgccggt cgaccaggcg cacgctgcgg cgctgacccc gcacgaggca cggcacgacg
     3481 cggcgcacgc cgggcagcag gaccgggtct gaacgagccc ttagtccgcc aaatcgaacg
     3541 cctcgtggaa ggcccgcgtc agatcgcgct cctgctcctc gagaagcgcc ccgaggggcc
     3601 ggatcaatgc gcgccggggg acggtgacaa tcgagtccag gttggccacg cacgggtgtt
     3661 cgagcccatt gtgaggcccg agggtgacct cgctgctcag gccccggacg gtcgtggtga
     3721 tcggtgccac cgtgatcagc tgccgcaccg ccctgacttc ctcgcgcgtc agcaccagga
     3781 ccgggcgcgt cttgtcgaga cgggccagcc agatcggatg catcagtcca gctcggacca
     3841 ggcgctgccg gcagtgcctg cagcccatgc cgcgtaggca tcggattcga ggtcagggtc
     3901 ggtggtcgag gcgtagatct gcccgtctcg tgccgcccgt tggcgccgta tctccccccg
     3961 gatcgctcgg ttgatcacat ccgcccggct cgtggtccgc gcagcggcct gcgcatcgac
     4021 gaaggcggcg aggtcgtcgt cgacccgaat cgtcatctgc acacccatac cgcgatcata
     4081 ccgctgcggt atgacagctg tcgacgccca gtcctcgcgt cgatctcagt ccgcggcaac
     4141 cacggcgtag atgcgtccgc cgcccgggac gggggcccgg ccgcccatgt caccgacata
     4201 caactgcccg gcggcgtcgc gcatcagcga ccagaagacc ccgtcggggc ggggatcggc
     4261 gaacgcccca ctcggccgcg acgaggcgtc gcgcagggcc tccggggcgg cgtcgcgggc
     4321 cacgccggcg accacgggcc ggcgtcgggg atgcgcagtt cgaggtctcc ccatcggatg
     4381 cagtgcccca gatgcgacaa caagggcgtc cactcctccg cccgtaaact cgcggctcag
     4441 ccggtcgtac acggcgtccg gggcgtcgcg tgcgcccgcc cattgactca ccaggtgacg
     4501 gtcgaaccag gtgtccacct tatggatcag gcggtggtgg ccgtcggcgc cgtcggctcg
     4561 ggcggcggcg agccaggccg gcaggtcccg cctggggcgg gggaacgtcc gcagttcgta
     4621 gcccagccag ccggacgggc aggtatgcag tgcctcggcg gcgtcgcgcc ggtcggggat
     4681 catcgctgcg agagtcacca cgtcgacgcc gatgcggggc aggggccgca ggtagttcgc
     4741 gatcagcaac tcggtgcccg attgccgtcg gcgcgcgacc cgggccggcg agcggttgcg
     4801 gccgcagcat cgcgcccatc gcctggaccg cctccgcgag cggcccgatg gtgcgcgcca
     4861 gccgcacgcg gccgacgtcc tcggcggtga accggagcac catcacctgg ccagtatggc
     4921 cagccggcgg cccgtgactc acaggtggag cacagccggt tgctcgctgc gggtcaggcc
     4981 cgcccggtgg aatcggggca tgagttccca tctcgaccgg cgatccgccc tgcgctgggg
     5041 cggtggcgcc ctgctgctgg ccggactggc cggctgcaca gcaacggccg ccaccgactc
     5101 cggcgacgcc tcaagcaacg gctccggcgc gacgacggcg gccggcgggg cggcgagtgc
     5161 ccgcccggcc gcgagcggcg ccggcgtgtt cgactcggcc acactgcata ccctcgacat
     5221 cgcgatgagc gaggccgact tcgcgggcgt gctcgcggca taccaggccg atcagagcaa
     5281 agagtgggtc gaggcgagcg tgacgatcga cggcaccagc tacgagcggt gcggggtccg
     5341 gctgaagggc aactcgacga tctggcgggt ggcggatggg gcgagcgcgt cggagtaccc
     5401 gtggctggtg cgactcgaca agtacgtcga cgggcaggca cacgacgggg tgaccgagat
     5461 cgtcgtgcgc ctgaacaaca ccgcctcggc gctgaacgag gccttgtcgc tggacctgct
     5521 ggccaaggcc gggctggcgt cgcagccctg gtcgtatgcc gcggtgtcgg ccgccggggc
     5581 cacgcccgtg ttgcggctga tcctggagca cccgtcggac ccctgggtgg cgcgggagtt
     5641 cggcgaggac ggcgtgctct acaaggcgga ggcgacgtcg aactacgact acctgggcga
     5701 cgagccgtcg ctctatgacg agcgcttcga cgtcgaggcg ggcgatgacg acctggcccc
     5761 gctcatcgaa ttcctcaagt gggtcaacga gatcgacgac gccgggttcg ccgccgacct
     5821 gtcgacgcat gtggatgtgg cggccttcgc gagctatctg gcgctggaag atctgatcgg
     5881 caactacgac gccatcgacg ggcccggcaa caactcctac ctgtggtggg ccaaggcggc
     5941 ggacctgatg accgtggtcg gctgggacca caacctgacc ttcggcgtga cgaaccgccc
     6001 tgggggtcaa ggcggtggcg ctcccggagg gccaggcggt atgccggccg gtggcgccaa
     6061 tcgcggcaac cagagcggca atgtgctcgc ccgccgcttc gaggccgacg cgacattcgc
     6121 cgcccagaaa tcggccgcga cggagcggct gcgcgccgag ctctatacga gcgggtatgc
     6181 ggcgcaacgc ctttcggaga tcgccgcgat gctgaccgag gtggccggtc acaacatcgg
     6241 cgccgagacc atcacctccg agaaggccgc catcgccacc tacttggcct gacgtgggtg
     6301 cgtggtctga agcggctacg tggtctgacg cgggaccgca cttttccgcc cagggcggaa
     6361 aagttgtgac gggcgtgact accaggggtg gggcgcgaag tccttgagga agacgccgaa
     6421 gaggtcctcg ccggcctcgc cgcgcacgat cgggtcgtag acgcgcgcgg ccccgtcgac
     6481 gaggtcgagg ggggcgtgcc agccctcggc ggcgatgcgc agcttctccc ggtgcgggcg
     6541 ctcgtcggtg atccatccgg tgtcgacggc ggtcatcagg atcttgtcgg tctcgaacat
     6601 ctccttggcg ctggtgcgcg tgagcatgtt gagggcggcc ttggccatat tcgtatgggg
     6661 gtgcccgggg cctttgtagc gccgagagaa ctggccctcc atggccgaga cgttgacgac
     6721 ataggcccgc cgggcgccgc gctcgacggc agcccgcatg ctcggccgca gccgcgagac
     6781 gagcaggaag ggcgcgaccg agttgcacag ctgcacttcc agcagctcca gcgggtcgac
     6841 gtcctcgacc ttctgggtcc aggagttgac gaggacatcg tcgggcagca ggccgccggc
     6901 gtcgatggcc gtgccggcca cgtgcctgtc cagactcgcc gaacccgcct gcagcgcaac
     6961 agaagtgagg gacgccgccg acaccgctcc cccaccgtcc gggcccccac cgtccgtgcc
     7021 ttcaccgtcc gtgccttcac cgtccggatg agtgtgggcg tgttcggcga gcactccgac
     7081 gagcgacgcg gggtgcgcct cgctgatccg atcgaacgcg atgaggcgcg gcagcggcat
     7141 accggcggga agcggttcgc cttctcccgc aacgagatgc gcataggcac cgggggtgcg
     7201 gcgcacggtc tggcaggcgt tgttgatgag gatgtcgagg ggtccgccgg cggcgacctc
     7261 gtcggcgagc gcgacgacct gggtggcgtc gcgcaggtcg atgccgacga tcgtcagccg
     7321 gtcgagccat tccgcggagt cggagagttc ggcgaagcga cgtgcggcat cgcgcgggaa
     7381 tcgcgtggtg atcgtcaggt cggcgccatc gcgcagcaac atcaaggcga tgtacatgcc
     7441 gatcttcgcc cgcccaccgg tgagcagggc gcgcctgccg cgcaggtcgg tgtgctggtc
     7501 gcgtttgccg tgactgaagg tggcgcaccg cgggcagagc cagtgataga aggcgtcgac
     7561 gagggtgtac ttctcgtggc agatatagca gccgcgtggg gtttcgagtt ccccggcata
     7621 cggcgaatcc gcttgcgcca caagcggtat gccgcgggtc tcgtcatcga tccgcagcgg
     7681 cgacccggtg gccgtggact ccaggaccgc cttgtcgcgc tcccgacccg gcgctaacgc
     7741 cgcctgccgc gcctcccgcc gccgcgcctt gcggatgcgg ctggccatga caccagtcgc
     7801 gcgcttgacg gtctggatgt cggggtcgtc gggcgcgagt tcgtgcaggc cggccaggac
     7861 gcgaagagtg gtttcgaggt cgtcaggatc cagtgtcgga tattccggct ccgcctcggt
     7921 caccggcaaa tcgtatgcgg gcctagaggc cgcgatcggg aaccggcggg accccggcga
     7981 cgtcgaaggt ccatgaggac cgctccgctc gccatcgctt ccgcgctcgt cctgctgctg
     8041 acggcctgcg gcacgcaagt ggccgacccc ggagcgagtg ggtccggcgg ggcggcaccg
     8101 accaccggga cgggggctgc cgggatgccg cccggggcga cggctactcc ggccggcggt
     8161 ggctcgtcat cggcggcgaa atccgggcca ccgtcggcga aggccacgag cggcacgggc
     8221 gagttcattg cggccgcgac cgtgctggag aagaagggtg aggggcctca gctgtgcctg
     8281 ggcggcgtcg cagagtcgct gcctccccag tgcggtggcc cggtcatcga caactgggac
     8341 tgggccacgg cgccgaagtc cgagacggcg gcgggtgtgc ggtggggcga atacctggtg
     8401 atcgggacgt acgacgcggc cgcctccacc ttccacctca ccaaacccgc caccacgcgc
     8461 gaggagtacg acggccccct gccgacggcg ccgccggatg ccatgttcac caccccctgc
     8521 ccggtccctg agggcggctg gcgggtcgtc gacccggcca aggccacccg agcggcgcag
     8581 gacgcggccc tcgaagcggc ggccaaactg ccgacgtatg ccgcggcgtg gctggaccag
     8641 tcgatcaacc cggccgaccc cgagaagcag ccggaggcta tgaacgatcc ggtcaagctc
     8701 attctcaacg tcaaggtcac cacggatgca gcgggcgccg agaaacagct gcgagagatc
     8761 tggggcggga tgctctgcgt cagcgagggt ggccacccgg aggctgagtt gcggaagatc
     8821 caggaggaac tcagcgggaa gcacggcatg ctcttcagcg ccgtcgacgg ccagacgagc
     8881 acggtggagt tcggggtgat ctgggacgac gggagcctgc agaaggcgat ggacgcggca
     8941 tacggtctcg gtgtcgtcca cgtgacgagc gccctgcagc cctaccccgg ctgaccgacg
     9001 gcggcgccgg accccaccgc gtacgtgggc tgcgtcaccg aatttaccct aatgcttgcg
     9061 cgagtaagca cccaggcggg agactaacct gagaagcact aagcaagcgc ttactcagca
     9121 atgccgccct ccgcgatcaa aggggaccac catgggccac tacaaggcca acctgcgaga
     9181 catcgagttc aacctcttcg aggtcttcgg ccgcgatgag gtcttcggcc acgggcccta
     9241 tgccgaggtc gacaccgaca ccgccaagga gatgctgaag gaggtcgccc ggctcgccga
     9301 gaacgagctg gccgcgtcct tcgtcgaggc cgaccgcaac ccgccggtct tcgacgccac
     9361 caccaactcc gtcaccatgc ccgagtcctt cgccaagtcc tacaaggcct acatcgactc
     9421 cggcttctgg aatatcgacg tgcccggcga gctcgacggc acggtcgcgc cgccgtcgat
     9481 gaagtgggcg atgaacgaga tgatcctcgg cgccaacccg gcgatcgcca tgttcgccgc
     9541 gtcctactcc ttcggcaagc tgctctacat cctcggcacg gacgagcaga agaagatggc
     9601 gaagtggatc atcgagaagg gctggcactg cacgatggtg ctgaccgagc ccgacgcggg
     9661 atccgacgtc ggcgccggcc ggacgaaggc cgtgcagaac gaggacggca cctggaacgt
     9721 ctccggcgtc aagcgtttca tcacctccgg tgagtcggac atgaccgaca acgtcgtcca
     9781 cttcgtcctc gcccgccccg aaggcgccgg ccccggcacc aagggcctga gcctgttcat
     9841 cgtgccgaag ttccacgtcg acctcgagac cggtgaactc ggcgagcgca atggcgtcta
     9901 cgtcaccaat gtcgaggaca agatgggcct gaaggtctcc acgacctgtg aggtcacctt
     9961 cggcgacaag caccccgcca tcggcaccct cttcggcgac gtccacgacg gcatcgccca
    10021 gatgttccgc gtgatcgagc acgcccggat gctcgtgggc accaaggcga tcgccaccct
    10081 ctcgaccggc tacctcaacg ccctcgaata cgccaagaac cgggtccagg gcgccgacct
    10141 gacccagatg gccgacaagg ccgcgccgcg cgtgacgatc acgcaccacc ccgacgtgcg
    10201 ccgcagcctc atgctgcaga aggcgtatgc cgagggcctg cgctcgctgg tcctgttcac
    10261 cgcctgccag caggacatcg tcgaccaggc gcagctggaa tccggctccg agcagctcga
    10321 gaaggacagc cccgccgacc tggccagccg cgtcaacgac ctgctcctgc cgatcgtcaa
    10381 gggcctcggc tccgagcggg cctgggtgct gctgggcacc gagtcgctgc agaccttcgg
    10441 cggttcgggc ttcctcaagg aatacccgat cgagcagtac gtccgcgacg ccaagatcga
    10501 caccctctat gagggcacca cggcgatcca gggcctggac ttcttcttcc gcaagatcat
    10561 ccgcgacaag gcgcaggccc tgagcacggt gtcgatgcag atccaggagt tcgccaagga
    10621 cctcggccag cagggcggca agctcgacaa ggaccgtgag ctgttgagca agggcgtcga
    10681 ggacgtccag ggcatcgtca cggcgatggt cggacagctg atgtcgggcg acccgcgcac
    10741 cggcggcgac gtcacgaacg tctacaaggt cggccagaac acctcgcgtg tcctgcttgc
    10801 cgcgggtgac ctgatcgtcg gctggctgct gctgcgccag gccgcgatcg cgcaggagaa
    10861 gctcgacgcg ggcgccaccg gcgccgacaa ggacttctac accggcaagg tggcggcggc
    10921 gcagttcttc agccgcaatg tgctgccgaa gctggccgcc gagcgggcca tcgccgaggc
    10981 catggacaac tcgctcatgg aagtccccga gtccgccttc tgatccgcca cgaagtccga
    11041 cgtctgtcga gttataactc gacagacgtc ggactttgtt ttggccctca ggagtcctgc
    11101 gcggagagtt ccactgcctg ttccgtcggg atcggcgcgt atgccgcggc ccggtcgggt
    11161 ccgcggggcg cgtcgtatgg gcgggccggt tccgtgtcgt cctggagccc cttgggcgcc
    11221 gggtcgaggg agatgcttgc gctgacggta tggacgtgca gctccacgtg gtcttgcccg
    11281 ggccccggct cgccgacggg cggcagggtg gagcgcaccg tgccggagac ggaggagaca
    11341 tcggcccaga ccggcttgcc ctcctcgatg ccgagccgga cgcggccgga cgtggtgcgc
    11401 agatccgccc gcccacgcca gatgacgtcg atgccgatcg ccccggagcc gttgcggcag
    11461 cggatatccc cgctggcgct ggtgatgcgc aacttccccg aaccggattt ggcatccagg
    11521 ctgccaacca cgcggtcgag gtggatgctc ccggaacccg tggcgacctg accttcgccg
    11581 gtgagccggc cgatgtgcac cgacccggaa ccggacttgc cctgcagctt ctcgacgtgg
    11641 ccgatctcga tcgtccccga cccggactcg aacatgaggt ccgcggcccg gacgcagtgc
    11701 acgccgcccg aaccgcactt gagcgtcacc tcgcccagct cgccctccag atcgatgccc
    11761 gcggacccgc accgcgcgcg aacgctgctg ccgtcgggca cgccgatccg caccgtatgc
    11821 cgcccgaacc cccacccgcg ccacttcgga ggcgcgacac gcacgccgtc gccgcgatgt
    11881 tcgacggaga agtcgtccgc gtgtgggccg gtgacgtcga cactcgtcgt cgcctgggcc
    11941 gccgcgacga cgaccacttg gccgtgatcg aggtcgacct cgagcgcgac cgggccgggt
    12001 gtggtgaaaa cctcgtgaat gctcatctgc gtgcctccta gatccagccg ctgaggcggc
    12061 cccgtgagcc gccgtggggg aagtcctggt cggcgaatga ccggtcgccg ctgaggtcca
    12121 actggatccc cgtgggacca gtggcctggc gcagggtctt gacgatccag ctgttgaggg
    12181 attgaccgag tcggccggcc tgctcctccg cgcggtcctt gatgccctcg gggagtcgga
    12241 cggtgatccg ggcgaggtca ccttcatcaa gggccgcgtc ctggatcgag ccgccggacc
    12301 cagggggctg ggtggacagg cttccatcgc tgaccagagg cagcgtctcc ccgcgcgcga
    12361 caccctcgac cacgaagtcc accccgcggc cgctcagccg cgcggtgacg ctgcccgaag
    12421 gcagcgaact ggtgatctcg gcggcggcgt cggccaggac ctcgaacaac gtgagccgga
    12481 ctgcggcatc cagggctcgg ttgagccgat ccgccgcgtc ctggacgtct gccccggcgc
    12541 cggctgcggc ggagtccagt gcctcccgca accgctcgac atacggtgac atctccatga
    12601 cgccatggtg acgtcattat gacgccacgt caagggtcga taaacgtcac ccggacaaag
    12661 ggcggatttg actgcttgct taagcccgag agcggtcccg ccggcaccta ctctgctagg
    12721 cgccagacct acttcgagca tgaggagaag acacgcgatg tgggttggac tcggaatctt
    12781 cctgttggtc gtcggcgcga tcctgtcctt cgccgtccgg gacagcatcg acgccgtgga
    12841 cctgacgatg atcggttaca tcctcatggg cggcggtgcg ctcgccgtgc tgctctccct
    12901 catcatgggc agtcgccgga cggctgccgg cggctacgag agccgcaccg tctcgcacgt
    12961 cgaccccaac acgggcacgc gcgtggaaga gcaccgg
//
LOCUS       DAOPJG010000085        12981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5049_length_12981_cov_7.958301, whole genome shotgun sequence.
ACCESSION   DAOPJG010000085 DAOPJG010000000
VERSION     DAOPJG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5049_length_12981_cov_7.958301"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..547
                     /locus_tag="PKU75_09090"
     CDS             197..547
                     /locus_tag="PKU75_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPF81072.1"
                     /translation="MGKLSFLAGAAAGYVLGARAGTQRYEQIKTQANKLWSSDPVQTK
                     VSQATDAAKTKAAPFVADVVSDAAKTTANKLREVKDNVGGDKDEDLPAGIHRGTDGRL
                     HAEHNWGPGPGKLP"
     gene            complement(611..1228)
                     /gene="cysE"
                     /locus_tag="PKU75_09095"
     CDS             complement(611..1228)
                     /gene="cysE"
                     /locus_tag="PKU75_09095"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPF81073.1"
                     /translation="MLVSPTVREGGLLAAARHGIREDLDAAMHRDPAATSRLVVLLTS
                     AGLHAIWLHRLAHALWLRPGGKWPARLIAYLSRSFTGVEIHPGATIGRRFFIDHGIGV
                     VIGETAEVGDDVMLYHQVTLGGRSMERVKRHPTLGDRVTVGAGAKVLGPVYVGADAQI
                     GANSVVVKDVPAGAIATGVPAAYRFPTMPAEDPYEALFRDPALFI"
     gene            complement(1294..>2119)
                     /gene="cysK"
                     /locus_tag="PKU75_09100"
     CDS             complement(1294..>2119)
                     /gene="cysK"
                     /locus_tag="PKU75_09100"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPF81074.1"
                     /translation="YNPANSVKDRIGVAIIDAAEASGALKPGGTIVEATSGNTGIALA
                     MVGAARGYNVVLTMPESMSKERRALLRAFGAELVLTPAGEGMKGAVNRADEIVAEREG
                     SILARQFANEANPEIHRKTTAEEIWKDTDGGVDIVISGIGTGGTITGVGQVLKERKPG
                     LQMIAVEPEESPILNGGQPGPHKIQGIGANFVPEILDTSIYDEVLDINADQAVDWARR
                     AAKEEGLLVGLSSGAALAAADIVARRPENAGKTIVVIIPSFGERYLSTVLFSDLLD"
     assembly_gap    2120..2129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2407..4716
                     /locus_tag="PKU75_09105"
     CDS             2407..4716
                     /locus_tag="PKU75_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPF81075.1"
                     /translation="MSPLQIIGIALCFTIPLAGWALLARQIWRFSALYRAGQPAVRLD
                     NPTSRTWTLIKEFLGHTRMSRLPLVALAHWFTALSFLILFATLVNAFFQLIWADFRLP
                     VIGHFPPFEWLIEAFAWGGLIGILVLIVIRQLAHPRSAAGEHGRRSRFFGSTWWQAYY
                     VEFTILVVTICILLLRTLEAAMLKITHPEESQALHFPLTGWLAGMWDGLSLDTINNLV
                     YLVATIKIMVSFAWMITISLQPTMGVAWHRFLAFPNIWFKRESSGRTALGALQPMVKD
                     GQPVTMETLEDHPEDEELQLGAGKIEDFTWKGLLDFSTCTECGRCQSQCPAWNTDKPL
                     SPKLLMMTLREHANAKAPYLTAAAEHRTALLEADPDLAALTELPLIGQTGYDIAEPLT
                     AYNPHGPNAVIDQDVLWSCTTCGACVEQCPVDIEHVDHIVDMRRHQALVESAFPGELA
                     GLFKNLEGKGNPWGMGAKARLDWAKDLPFEVPVVGEDVENLDGYDYLFWVGCAGAYED
                     RAKKTTRAVAELLHLAEVKFAVLGNGETCTGDSARRAGNEFLFQMLAQQNVEVLAEAG
                     AAKIVVTCAHCFNTLKNEYPQFGGSYEVLHHTQLLNRLVREKRLVPVTRPGADPVKST
                     NKDAASSANTVTYHDPCYLGRHNHVYTPPRELLGSLPGVTLKEMPRHGERSFCCGAGG
                     ARMWMEEKLGERININRTNEALGTGAQRIAIGCPFCRVMLSDGLTARQSEGYFEQVEV
                     VDIAQMLLAAVRPGPEAQPAQIDVSSVAN"
     gene            complement(4799..5773)
                     /locus_tag="PKU75_09110"
     CDS             complement(4799..5773)
                     /locus_tag="PKU75_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPF81076.1"
                     /translation="MKTPAPQEFSPSPRGTDEVLYAAGGGTAYVRLNRPKALNSLTRV
                     MVEDLCIQLDEWVTDDRVTAIVLDGTGDRALCAGGDVKAVRAAIVNGDPDAAVDFWAR
                     EYDLALRLGTYPKPVTAHMRGVVMGGGLGLSAHVTHRPIGPDARVAMPETAIGFFPDV
                     GITRIFARTPGELGTYAAVTGATFGAADAVLLGLADPSGDLGPAPILVQQNWIDECFA
                     GDDPAAIIGRLESHPASEARECAAEVRRRSPLAVSAALARVRRAATEADLAETLAVDT
                     RLARGMMLAGDFTEGVRAKLVDKDDRPRWRHARIEDVTPADVDALFSA"
     gene            complement(5770..8199)
                     /locus_tag="PKU75_09115"
     CDS             complement(5770..8199)
                     /locus_tag="PKU75_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPF81077.1"
                     /translation="MLPPCLGRRDLLAHVRSSLIDRRWVTLVGPSGMGKSLIARHAVA
                     DREVAWVDARVVHDVDGLIGAWLQALNPDVAPGDAPLPALIRALDDTGRLSVLDGVSE
                     EQIPGLFELIEATLAATTLPRILSTSHTPSGSPHGLDLRVEPLPTPLPGQPLEGASLE
                     LFVGRLASAGGELVDLRRDEAVLRSLLEASGGIPLVIEQLAAQAAQVGLAAVAPTDSL
                     SDAIGRSYAMVDATSQRAFRRLGCVAFPVGLDVFAALAELPRALAIAAAAQLERHSLV
                     EITPDERVRMAAAARRHALEVTRHTDDAACVTAALLAWADAALPASEEEGSGSDPWLA
                     DYDAVDLAIAHGAGDPATRDLAYDMANRAFGPLYTAMRPRDALTLLETVLASGDGPPE
                     VGAQVARRAGICASEVLGTFEGLRFLDRAEQHADIALAPDVQRARIASIRAEMYLDAG
                     QLDAARTEVDRIFALGCEDTYAERQARRTLMDVETSAGNLATAEAIAPLIIDGATGEE
                     AWLGIAARVLLAQIAREQGRQVEALAAARAARHDAMARGEDRIALLADVLARRISCEP
                     PALTPEPEALPWAVRLGYQLQGARDAACEERFEHAAGIAADVVVLADSARLERDGIEA
                     RIVLADALLALGDTGQAASTYAAAARHAAACPLPLRIADAFDGLALCLRETQPTHAAR
                     CVWLGMQLRNAHGAAALSRPGVDTSRLPAPRAAKAWFADGRISAAGIAAAGRIEGGEA
                     DAAADSPLASLTKAQLAVAQLVGAGLTSKEIAEQLYLSPRTVDNHLAQIYRKLDIPSR
                     SRLAAMMAKSA"
     gene            complement(8516..9484)
                     /locus_tag="PKU75_09120"
     CDS             complement(8516..9484)
                     /locus_tag="PKU75_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPF81078.1"
                     /translation="MRRPALIFTVTTASALTLAACGSSSDPMASGSSTSSASAATSAA
                     TSADSSSGGATSSAQSIAVGSADFSESQLLAEIYAGALQAKGINATAKLNIGSREVSV
                     KALQDGSIDVMPEYTGAMAFYYDKNFTETDPQAVYDALKGMLPADLTLLQASAAEDND
                     SINVTKATAQKYQLTSVADLAAHAGDLTLGAPPEFKLRPQGIPGLEKTYGVTFGTFRP
                     LKGAALVNALKNGQVDAANIFSTDPAIAANDFVTLTDDKRLFGAQNIVPLVRADAATP
                     EVSAALDAVNAKLTTATLSDLLKKTDIDKQDPATVAKEFLTANGLG"
     gene            complement(9611..10348)
                     /locus_tag="PKU75_09125"
     CDS             complement(9611..10348)
                     /locus_tag="PKU75_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF81079.1"
                     /translation="MIAGIWQWLSDPANWTGPDGIPVRTLEHLRYSLIVLLAAAMIAI
                     PTGLYVGHTGRGRWLVTAANALRAVPTLGLLFALSLWLGPKIHGNLAFSLPSIIVLVL
                     LAVPPLLAGAYAGVEAVDPAARDAARGMGMRPGEVLRHVELPVSLPLLLSGVRAAALQ
                     VIATATIAAYVGLGGLGRFLLDGLSLADYPATAGGALLVSALALIVDLVLAAVTRLVV
                     SPGLTGKYRGASDVDIQTSRTAAAIEH"
     gene            complement(10345..11004)
                     /locus_tag="PKU75_09130"
     CDS             complement(10345..11004)
                     /locus_tag="PKU75_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF81080.1"
                     /translation="MTWVLDHLGLIWELTVRHTVLAGIPLILGLLLSIPLGWLARRYR
                     VLAPIALAGTGLLYTIPSLALFILMPLILGTKILDPINVVVAMTVYTVALLVRTVVDG
                     LASVPDHVDAAAQAMGLGRLRRLFTVQLPLAVPVIAAGLRVAAVSNVSIVSIASIIGV
                     SQLGSLLTDGYNRDIPGELWAGTLACLLLALLFDAVIQAGAWALTPWRRAGAVPTGAE
                     P"
     gene            complement(11001..12125)
                     /locus_tag="PKU75_09135"
     CDS             complement(11001..12125)
                     /locus_tag="PKU75_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF81081.1"
                     /translation="MIRFDAVTKRYPGGTTAVDDLTLEAPTGQITVLVGPSGCGKTTS
                     LRMVNRMIEPTSGRISIDDTDTASMDPASLRRGIGYVIQHGGLFPHHTIERNVATVPL
                     LLGSDKKTAYARAHELLERVGLPREFAQRYPAQLSGGQQQRVGVARALAADPPVMLMD
                     EPFSAVDPVVRAQLQEEFKRLQSDLGKTILFVTHDIDEAITLGDQVAVMRVGGRLAQI
                     ASPSELLAHPADDFVADFVGRDRGYRALSFVDGSVPLVPEASVRPGDPGEVARERSVD
                     GWVLVADGGQPLGWLAVDRVGAQVDVADLALGGTLARQGGPLRAALDAALSSPSGRGV
                     VVGDQGELLGTVRARDVIDVIEGSRGGSGVQDTPAPGVSP"
     gene            complement(12198..12524)
                     /gene="cutA"
                     /locus_tag="PKU75_09140"
     CDS             complement(12198..12524)
                     /gene="cutA"
                     /locus_tag="PKU75_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HPF81082.1"
                     /translation="MTEIVEVRSSAPDPATAQALADLLVAERLAACVQCLSGLASTYR
                     WDGGIQHEQEILLLAKTTAARLPALMARLRAAHPYDVPEILATPTTADAPYAAWVAEQ
                     TTAPGG"
     gene            complement(12524..>12981)
                     /gene="dcd"
                     /locus_tag="PKU75_09145"
     CDS             complement(12524..>12981)
                     /gene="dcd"
                     /locus_tag="PKU75_09145"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HPF81083.1"
                     /translation="RLFDNHKYPVIDPAQDQPDLTRLVEVDPDEGFVLHPGEFVLGST
                     LETVTLPDDLAARVEGKSSLGRLGLLTHATAGFVDPGFSGHVTLELSNVATLPIMLWP
                     GMKIGQLAFFRLSSSAENPYGSAAYGSHYQGQRGPTASRSFQNFHRTDV"
BASE COUNT         1991 a         4426 c         4690 g         1864 t
ORIGIN      
        1 tgccgccata gcggttttca agaccgccgc actaggccac tatgcgagcc ctcctgggcc
       61 gccgggggcg gccgcgggtc aacggtacgc gaacacctgg ccgggtgccg agccaccacg
      121 gacagcggac ggcggctagg gtcgaaggcc aggagtcggg ccacggcata ccgtggcgag
      181 cagaaggagc gaacgaatgg gcaagctgtc attcctggcc ggagcggccg ccggctatgt
      241 gctcggtgcc cgcgctggca cgcagcgtta cgagcagatc aagacccagg cgaacaagct
      301 gtggtcgagc gacccggtgc agaccaaggt gagccaggcg accgacgcgg ccaagaccaa
      361 agccgccccc ttcgtcgcgg acgtcgtcag cgacgccgcg aagaccaccg ccaacaagct
      421 gcgcgaggtc aaggacaacg tcggcgggga caaggacgag gacctgcctg cgggaatcca
      481 tcgcggcacc gacggccgac tccacgccga gcacaactgg ggaccgggac cgggcaagct
      541 tccctgagcc cccgcagacg acgaaccgcc ggctgcccag agtgggcaac cggcggttcg
      601 tcgtctgcgg ctagatgaaa agcgcgggat cgcggaagag cgcctcgtaa gggtcctcgg
      661 cgggcatcgt cgggaagcgg tatgccgcgg gcacgcccgt cgcgatggcg ccggcgggga
      721 cgtccttgac gacgaccgag ttggcgccga tctgggcgtc ggcgccgaca tagaccggtc
      781 ccagcacctt ggcccccgcc ccgacggtca cccggtcacc gagggtgggg tggcgcttga
      841 cccgctccat cgagcgccca ccgagcgtga cctggtgata cagcatcacg tcgtcgccga
      901 cctcggccgt ctcgccgatg acgacgccaa tgccgtggtc gatgaagaag cgccgcccga
      961 tggtcgcccc cggatggatc tccactccgg tgaacgaccg cgacagatac gcgatcaacc
     1021 gcgcgggcca cttgccgccg ggccggagcc acagcgcgtg ggcgagccgg tgcagccaga
     1081 tggcgtgcag gcccgcggag gtcaacagca cgacgagccg cgacgtggca gcggggtcgc
     1141 ggtgcatcgc cgcgtcgagg tcttcacgta tgccgtgacg cgccgcagca agcaggccgc
     1201 cctcgcggac ggtgggagac acgagcatcg ctttcacctt catggtcggg accgccggcc
     1261 gggcaccctg gaagcacccg ggcccggctt gggtcagtcg aggaggtcgg agaagaggac
     1321 ggtggacagg tagcgctcgc cgaagctggg gatgatcacc acgatcgtct tgccggcgtt
     1381 ctcggggcgg cgggcgacga tgtcggcggc ggccagggcg gcgccggagg acagcccgac
     1441 gagcaggccc tcctccttgg cggcgcgccg ggcccagtcg acggcctggt cggcgttgat
     1501 gtcgaggacc tcgtcataga tggacgtgtc gaggatctcg ggcacgaagt tggcgccgat
     1561 gccctggatc ttgtgcgggc caggctggcc gccgttgagg atcggggact cctctggctc
     1621 gaccgcaatc atctgcaggc cgggcttgcg ctccttgagg acctggccga caccggtgat
     1681 ggtgccgccg gtgccgatgc cggagatgac gatgtcgacg ccgccgtcgg tgtccttcca
     1741 gatctcctcc gcggtggtct tgcggtggat ctcggggttg gcctcgttgg cgaactggcg
     1801 cgccaggatg gatccctcgc gctcggcgac gatctcgtcg gcgcggttga ccgcaccctt
     1861 catgccctcg cccgccgggg tgagcaccag ctccgcgccg aacgcccgca gcagggcgcg
     1921 gcgctccttg gacatcgact cgggcatggt caggacgacg ttgtagccgc gggccgcccc
     1981 gaccatggcc agcgcgatgc ccgtgttgcc ggacgtggcc tcgacgatcg tgccgccggg
     2041 cttgagggca ccggacgcct cggcggcgtc gatgatcgcg acgccgatgc ggtccttgac
     2101 ggagttggcc gggttgtagn nnnnnnnnnc ctcgctgtcg atgatgcggt tgatccgcac
     2161 gagcggggtg ttgccgatga gcttggtgac gtcgtcgtag ataggcactg cttctccgta
     2221 tggtgcgtgg tccagcgggt cgtgtacgcg gcaacactac ccaaccgcga tttatgcccg
     2281 cacaaacatc tcgttatatg tcatgcgtaa ccgcatgcgg caaatcgccc ggggcgacgg
     2341 acaccacatg agccggtcat cacaggcgcc ccatccgcac cccgctgcct acttagactc
     2401 gcgatcatgt cccctctgca gatcatcggg atcgccttgt gcttcaccat ccccctcgcg
     2461 ggatgggcct tgctcgcgcg gcagatctgg cgcttcagcg ccctctatcg cgccgggcaa
     2521 ccggcggtgc gcctggacaa cccgacgtcg cggacctgga ccctgatcaa ggagttcctc
     2581 gggcacaccc gcatgtcccg gttgccgctg gtcgcactgg cccactggtt caccgcgctg
     2641 agcttcctga tcctgttcgc caccctggtc aacgccttct tccagctcat ctgggcggac
     2701 ttccgcctgc cggtcatcgg tcacttcccg cccttcgaat ggctcatcga ggccttcgcg
     2761 tggggcggcc tgatcgggat cctcgtgctc atcgtcatcc gccagctggc gcacccgcgc
     2821 agcgccgccg gtgagcacgg ccgccgctcc cgtttcttcg gctcgacctg gtggcaggcc
     2881 tactacgtcg agttcaccat cctcgtggtg acgatctgca tcctgctgct gcgcaccctc
     2941 gaggcggcga tgctcaagat cacccacccg gaggagtcgc aggcgttgca cttcccgctc
     3001 accggctggc tggccggcat gtgggacggg ctctcgctcg acacgatcaa caacctcgtc
     3061 tacctcgtcg cgacgatcaa gatcatggtc tccttcgcct ggatgatcac catctcgttg
     3121 cagccgacga tgggcgttgc ctggcaccgc ttcctcgcct tcccgaacat ctggttcaag
     3181 cgcgagtcct ccggccgcac cgccctcggc gcgttgcagc cgatggtcaa ggacggccag
     3241 cccgtgacca tggagaccct cgaggaccac cccgaggacg aggaactcca actcggggca
     3301 ggcaagatcg aagacttcac ctggaagggc ctgctcgact tctccacctg caccgagtgc
     3361 ggccgctgcc agagccagtg cccggcgtgg aacaccgaca agcccctctc ccccaagctg
     3421 ctgatgatga cgctgcggga gcacgccaac gccaaggcgc cctacctgac cgcggcggcc
     3481 gaacatcgta cggccctcct cgaagccgac cccgatctgg ccgccctgac cgaactcccc
     3541 ctcatcggcc agaccggcta cgacatcgcc gaaccgctca cggcatacaa cccgcacggc
     3601 ccgaacgccg tcatcgacca ggacgtgctg tggtcctgca cgacctgcgg cgcctgcgtc
     3661 gagcagtgcc ccgtcgacat cgagcacgtc gaccacatcg tcgacatgcg ccgccaccag
     3721 gcgctcgtcg aatccgcctt cccgggcgag ctcgccggcc tgttcaagaa cctcgagggc
     3781 aagggcaacc cgtggggcat gggcgcgaag gctcgcctcg actgggccaa ggacctcccc
     3841 ttcgaggtgc cggtcgtcgg cgaggacgtc gaaaacctcg acggctacga ctacctgttc
     3901 tgggtcggtt gcgccggggc ctacgaggac cgcgccaaga agacgacccg cgcggtcgcc
     3961 gagttgctgc acctcgccga ggtgaagttc gccgtcctcg gcaacggcga gacgtgcacg
     4021 ggcgactccg cccgccgcgc cggcaacgag ttcctcttcc agatgctcgc gcagcagaat
     4081 gtcgaggtcc tcgccgaggc gggcgccgcc aagatcgtgg tcacctgcgc gcactgcttc
     4141 aacacgctga agaacgagta cccgcagttc ggcgggtcgt atgaggtgct gcaccacacc
     4201 cagctgctca accgtctcgt gcgcgagaag cgactggtgc cggtcacccg ccccggtgcc
     4261 gaccccgtga agtcgacgaa caaggacgcc gcctccagcg cgaacacggt gacctatcac
     4321 gacccgtgct acctcggccg gcacaaccac gtctacaccc cgccccgcga actgctcggc
     4381 tccctcccgg gcgtcacgct caaggagatg ccgcgccatg gagagcggtc cttctgctgc
     4441 ggcgccggcg gcgcgcgcat gtggatggag gagaagctcg gcgagcggat caacatcaac
     4501 cgcaccaacg aggccctcgg caccggcgcc cagcgcatcg ccatcggctg ccccttctgc
     4561 cgcgtcatgc tctccgacgg gctgaccgcc cggcagtccg agggctattt cgagcaggtg
     4621 gaggtcgtcg acatcgccca gatgctgctg gccgccgtgc gcccggggcc ggaagcccaa
     4681 ccagcccaga tcgacgtctc gtcggtcgcg aactgacatc tcgccgcggt cgaaacggca
     4741 tctcgccggg gtcgaaatga catctcgacg gagggtcggg tggcgagcgg cggtgcggct
     4801 aggcggagaa gagggcgtcg acgtcggcgg gggtgacgtc ctcgatccgg gcatgccgcc
     4861 agcgtggccg gtcgtccttg tccacgagtt tggcgcgcac gccctcggtg aagtcgccgg
     4921 cgagcatcat cccgcgcgcc aacctggtgt cgacggcgag ggtctcggcg agatcggcct
     4981 cggtggccgc gcggcgcacg cgcgccagcg cggcggacac ggcgagcggg gaacgccggc
     5041 gcacctccgc agcgcactcg cgcgcctcgg aggcggggtg cgactccagc cggccgatga
     5101 tcgccgcggg gtcgtcaccg gcgaagcatt cgtcgatcca gttctgctgc accagaatcg
     5161 gcgccggtcc caaatcgccg gacgggtcgg ctaggccgag caacaccgcg tccgccgcgc
     5221 cgaaggtggc gccggtgacg gcggcatacg tcccgagctc ccccggcgtc cgggcgaaga
     5281 tgcgggtgat gccgacgtcg gggaagaacc cgatcgccgt ctcgggcatg gcgacccggg
     5341 cgtctgggcc gatgggccga tgcgtgacat gggcgctcag cccgagcccg ccgcccatga
     5401 cgacgccgcg catgtgagcc gtgaccggct tgggataggt ccccaggcgc agggcgagat
     5461 cgtactcgcg tgcccaaaag tccactgccg catcgggatc gccattgacg atggcggcgc
     5521 ggacggcctt gacgtcgccg ccggcgcaca gtgcccggtc gccggtgccg tcgaggacga
     5581 tggccgtgac ccggtcgtcg gtgacccact cgtcgagttg gatgcagagg tcctcgacca
     5641 tgacgcgggt caggctgttg agcgctttcg gccggttgag ccggacgtat gccgtgccgc
     5701 cgcccgcggc atacagcacc tcgtccgtgc cccgcggcga tggcgaaaac tcttgtggcg
     5761 caggggtttt catgcgctct ttgccatcat cgcggcgagg cgggaccgcg acgggatgtc
     5821 gagcttgcga tagatctggg ccaggtggtt gtcgacggtg cgcggcgaga gatagagctg
     5881 ctcggcgatc tccttgctgg tcagcccggc gccgacgagt tgggcgaccg cgagttgcgc
     5941 cttggtcaga ctcgcgagcg gcgagtcggc cgcagcgtcg gcctcgccgc cctcgatccg
     6001 cccggccgcg gcgatgccgg ccgccgagat gcgcccgtcg gcgaaccacg ccttggccgc
     6061 gcgcggcgcg ggcagccggc tggtgtccac gcccggccgc gacaaggcgg cggcaccgtg
     6121 cgcgttgcgc aactgcatac ccagccacac gcaccgggcg gcgtgggtgg gctgggtctc
     6181 cctcaggcac agagcgagcc cgtcgaaggc gtcggcgatc cgcagcggga ggggacacgc
     6241 ggcggcgtgc cgggcggcag cggcatacgt gctggcggcc tgcccggtgt cgcccaaggc
     6301 caacaaggcg tcggcgagca cgatccgcgc ctcgatcccg tcgcgctcga ggcgggcgct
     6361 gtcggcgagc acgacgacat cggcagcgat gccggccgcg tgctcgaagc gctcctcgca
     6421 cgcggcatct cgggcaccct gcagctggta gccgagccgg acggcccacg ggagcgcctc
     6481 gggttcgggg gtcagggcgg gcggttcgca ggagatccgc cgggcgagga catcggccag
     6541 cagggcgatc cggtcctcac cgcgggccat cgcatcgtga cgtgccgcgc gagcggcggc
     6601 cagcgcctcg acctgccggc cctgctcgcg ggcgatctgg gccagtagca cgcgggcggc
     6661 gattccgagc cacgcctcct cgcccgtggc gccgtcgatg atgaggggcg cgatggcctc
     6721 cgccgtggcg agattgcccg cgctcgtctc gacgtccatc agcgtgcgcc gggcctgccg
     6781 ctcggcatac gtgtcctcgc agccgagcgc gaagatccgg tccacctcgg tgcgggcagc
     6841 gtcgagttgg ccggcgtcga gatacatctc ggcccggatc gaggcgatgc gggcccgttg
     6901 gacatcgggt gccagggcga tgtcggcgtg ctgctcggcg cggtcgagga agcgcagccc
     6961 ctcgaaggtg ccgagcacct cggacgcgca gatcccggcc cgccgggcca cctgggcacc
     7021 gacctcgggc gggccgtcgc cgctggcgag cacggtctcg aggagggtga gggcgtcccg
     7081 cggacgcatg gccgtataca gcgggccgaa ggcccggttg gccatgtcgt aagccaggtc
     7141 gcgggtggcc ggatcgccgg caccgtgcgc gatggccaaa tcgaccgcgt cgtagtcggc
     7201 gagccacgga tcgcttcccg acccctcctc ctcgctggcc ggcagcgccg cgtcggccca
     7261 cgcgagcagc gcggccgtca cgcacgcggc gtcatcggta tgccgcgtca cctccagcgc
     7321 gtggcgccgg gccgccgccg ccatccgcac gcgttcgtcc ggggtgatct cgacgaggga
     7381 gtgccgctcc agctgggccg ctgcggcgat ggccagggcc cgcggcagct cggccagcgc
     7441 cgcgaagacg tcgagtccga cggggaaggc aacgcagccc agccggcgga aggcccgttg
     7501 gctcgtcgcg tccaccatgg cgtaggagcg cccgatggcg tcggagagcg agtctgtcgg
     7561 cgcgacggcc gcgagcccga cctgcgccgc ctgcgccgcc agttgttcga tgacgagcgg
     7621 tatgccgccg ctcgcctcga gcagggagcg cagcacagcc tcgtcccggc gcaggtcgac
     7681 cagttctccg ccggcgctgg ccaaccggcc gacgaaaagc tccagggagg cgccctcgag
     7741 gggttggccc ggaagcggcg tgggcagcgg ctccacccgg aggtcgagtc cgtgcgggga
     7801 gccggacggg gtgtggctcg tgctcaggat ccggggcagg gtggtggccg cgagggtcgc
     7861 ctcgatgagt tcgaacaggc ccgggatctg ctcctcggag acgccgtcga gcacactgag
     7921 ccggcccgtg tcgtccagcg cccggatcag ggccggcaag ggcgcatcac ccggggcgac
     7981 atcggggttc agcgcctgca gccaggcgcc gatgagcccg tcgacgtcgt gcacgacgcg
     8041 cgcatccacc cacgcgacct cgcgatcggc gacggcgtgc cgcgcgatca gcgacttgcc
     8101 catgccggat gggccgacga gggtcaccca ccggcggtcg atcaggctgg agcggacgtg
     8161 tgccagcagg tcacgtcggc ccaggcacgg gggcagcacg ccgctcaggt tactggacct
     8221 cgggagtccg ccgtcggtga gatctgaacc cggtgagcac agtggccaat tgcgccacct
     8281 gagcagacag attgagtgaa attaccgatg cgcagggggt cccgactgga caagagtggt
     8341 ggtcagccgg aacacctcgg acaaacgtcc cgagggatgg caagcccgtc ggagggaggg
     8401 gcttgccatc gcgctccgcg ccgtggaacg tcgggtgggg gcccggccgg ttccgccacg
     8461 aaggcgaccc ggggtctggg ggcagacccg ggtcgccttc ctcaatggtg caggctcacc
     8521 cgaggccgtt ggcggtgagg aactccttgg ccaccgtggc agggtcctgc ttgtcgatgt
     8581 cggtcttctt gagcaggtcc gacaaggtcg ccgtggtcag cttggcgttg accgcgtcga
     8641 gggcggccga cacttcgggg gtcgcggcgt cggcgcggac caggggcacg atgttctggg
     8701 cgccgaagag ccgcttgtcg tcggtgaggg tcacgaagtc gttggccgcg atggccgggt
     8761 cggtggagaa gatattggcc gcgtccacct ggccgttctt gagcgcgttg accaaggccg
     8821 cacccttcag gggccggaag gtgccgaagg tgacgccgta ggtcttctcc aggccgggga
     8881 tgccctgtgg gcgcagcttg aactccggcg gcgccccgag ggtcaggtcg ccggcgtgcg
     8941 cggccaggtc ggccaccgac gtcagctggt atttctgcgc cgtcgccttg gtgacattga
     9001 tcgagtcgtt gtcctcggcc gcagacgcct gcagcagcgt caggtcagcg ggcagcatgc
     9061 ccttgagcgc gtcatacacg gcctgcgggt cggtctcggt gaagttcttg tcgtagtaga
     9121 aggccatcgc gccggtgtat tcgggcatga cgtcgatcga gccgtcctgc agcgccttga
     9181 ccgacacctc ccgcgagccg atgttgagct tggccgtggc gttgatgccc ttggcctgca
     9241 gggcgccggc gtagatctcg gcgagcaact ggctctcgga gaagtccgcg gagccgaccg
     9301 caatgctctg cgccgagctc gtcgcgccgc cggagctgct gtcggcgctc gtcgcggcac
     9361 tcgtggcggc cgacgcgctc gaggtcgacg acccggaggc catcgggtcg gatgaggatc
     9421 cgcaggctgc cagggtgagg gcggaggcgg tggtcacggt gaagatcagc gcgggtcggc
     9481 gcatcgggaa actcctctag taggtcgggc gggcaggtcc cgctccggcg tgtcgggttc
     9541 gctggatcag gtttcgctgg atcacgtgtt cacatggatc actgctcaca ttgatccgtg
     9601 ctcacagtga tcagtgctcg atggccgcgg cagtgcggct ggtctggatg tcaacgtcgg
     9661 acgcgccgcg atacttcccc gtcagcccgg gactgacgac caggcgggtc acggcggcga
     9721 gcacaaggtc gacgatcagc gcgagcgccg agacgagcag cgcgcccccc gccgtggccg
     9781 gatagtcggc gagggagagc ccgtcgagca ggaaccggcc caggccgccg aggccgacat
     9841 aggcggcgat ggtggccgtg gcgatcacct gcaacgcggc ggcgcggact ccggagagca
     9901 gcagcggcaa ggacacgggc aactcgacgt gccggagcac ctcaccgggc cgcataccca
     9961 ttccgcgggc ggcgtcgcgg gccgccgggt cgacggcctc gacaccggca tacgcgccgg
    10021 cgagcagcgg cgggaccgcc agcaggacca gcacgatgat gctcgggagg ctgaaggcga
    10081 ggttgccgtg gatcttcggg ccgagccaca acgagagggc gaaaagcagt ccgagggtgg
    10141 gcacggcccg cagcgcgttg gcggcggtga ccagccagcg accccggccg gtgtgaccga
    10201 catacaggcc ggtggggatg gcgatcatcg cggcggcgag caggacgatg aggctgtagc
    10261 gcaggtgctc cagggtgcgc acgggtatgc cgtccggtcc ggtccagttg gccgggtcgc
    10321 tcagccactg ccagatgccg gcgatcacgg ctcggccccg gtcgggacgg ctccggcgcg
    10381 ccgccaaggg gtgagcgccc aggcgccggc ctggatgacg gcgtcgaaga ggagggcgag
    10441 cagcaggcag gcgagggtgc cggcccacag ctcgccgggg atgtcgcggt tgtagccgtc
    10501 ggtcaggagc gagcccagtt gcgagacacc gatgatcgag gcgatcgaga cgatgctgac
    10561 gttgctcacc gctgccaccc gcaggcccgc ggcgatcacc ggcacggcca gcggcagttg
    10621 cacggtgaac agccgccgca gccggcccag gcccatcgcc tgcgcggcgg cgtcgacatg
    10681 gtccggcacc gaggcgagtc cgtcgacgac ggtgcgcacg agcagagcga ccgtatagac
    10741 ggtcatcgcg accacgacat tgatcgggtc gaggatcttg gtgccgagga tcagcggcat
    10801 gaggatgaag agcgccaggc tcgggatcgt gtagagcagc ccggtgccgg cgagcgcgat
    10861 gggcgcgagg acgcggtagc ggcgggccag ccagccgagc gggatgctca gcagcaggcc
    10921 gaggatcagc gggatgcccg cgaggacggt atgccgcacc gtcagctccc agatcagccc
    10981 caggtggtcg agcacccagg tcatggcgaa acccctggtg ccggcgtgtc ctgaacgccg
    11041 ctaccgccgc gcgatccctc gatgacgtcg atgacgtcgc gggcccggac cgtgcccagc
    11101 agttccccct ggtcaccgac gaccaccccg cggccgctcg gcgaggacaa ggcggcatcc
    11161 aaggccgcac gcagcggacc gccttggcgt gcgagcgtcc ctccgagcgc cagatcggcg
    11221 acgtccacct gcgcgccgac ccggtcgacg gccagccagc cgagcggctg cccgccgtcg
    11281 gcgacgagca cccagccgtc gaccgaccgc tcgcgcgcca cctcccccgg gtcgccgggc
    11341 ctgaccgacg cctccggcac cagcggcacg ctgccgtcga cgaagctcag cgcgcgatag
    11401 ccgcggtcgc ggccgacgaa atcggcgacg aagtcgtcgg ccgggtgggc gagcagctcg
    11461 ctgggggagg cgatctgcgc cagccggccg ccgacgcgca tcacggccac ctggtcgccg
    11521 agggtgatgg cctcgtcgat gtcgtgggtg acgaaaagga tggtcttgcc gaggtcggac
    11581 tgcagccgct tgaactcctc ctgcaactgc gcccgcacga ccgggtcgac ggcggagaag
    11641 ggctcgtcca tgagcatcac gggcggatcg gcggcgagcg cgcgggccac gccgacgcgc
    11701 tgctgctgcc cgccggagag ctgggcggga tagcgctggg cgaactcccg cggcaggccc
    11761 acccgctcga ggagttcgtg ggcgcgggca tacgcggtct tcttgtcgga gccgagcagc
    11821 aatggcacgg tggcgacatt gcgctcgatc gtgtggtgcg ggaagaggcc gccgtgttgg
    11881 atgacgtagc cgatgccgcg gcgcaggctg gccggatcca tcgaggcggt gtcggtgtcg
    11941 tcgatgctga tccgccccga ggtgggctcg atcatccggt tgaccatccg caaggacgtc
    12001 gtcttgccgc acccggacgg tccgacgagc accgtgatct ggccggtggg tgcctcgagg
    12061 gtcaggtcat cgacggccgt ggtgccgccg ggatacctct tggtgactgc gtcgaaccgg
    12121 atcacgtgcg gtccttcccc gttggagcgc ccacgctaac ccagggcacc gacaaccggc
    12181 ggactaacag accaccctca ccctccgggt gctgtcgtct gctcggcgac ccaggcggca
    12241 tacggcgcgt cggccgtggt gggcgtggcg aggatctccg gcacgtcata cgggtgcgcg
    12301 gcgcgcaggc gcgccatcag cgccggcagg cgggcggccg tcgtcttggc aagcagcagg
    12361 atctcctgct cgtgctggat gccgccgtcc cagcggtagg tcgacgccag gcccgagagg
    12421 cactggacgc aggcggcgag gcgctcggcg acgaggagat cggcgagcgc ctgggcggtg
    12481 gcgggatcgg gcgcgctgga gcgcacctcg acgatctcgg tcatcagacg tccgtgcggt
    12541 ggaagttctg gaaggacctg gacgcggtgg ggccgcgttg gccctgatag tgcgagccgt
    12601 atgccgcact gccgtaagga ttttcggccg aactggacag ccggaagaag gccagttggc
    12661 cgatcttcat gcccggccac agcatgatcg gcagcgtcgc gacattggac agctcgagcg
    12721 tgacgtgacc gctgaacccg ggatcgacga atccggctgt ggcgtgggtg agcaggccga
    12781 ggcgacccag cgacgacttg ccctcgaccc gggcggccag gtcgtcgggg agggtcacgg
    12841 tctccagggt cgagccgagc acgaactccc ccgggtggag cacgaaccct tcgtcggggt
    12901 cgacctcgac gagccgggtc aggtcgggct ggtcctgggc ggggtcgatg acggggtatt
    12961 tgtggttgtc gaagagccgg a
//
LOCUS       DAOPJG010000086        12954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5065_length_12954_cov_8.229165, whole genome shotgun sequence.
ACCESSION   DAOPJG010000086 DAOPJG010000000
VERSION     DAOPJG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_12954_cov_8.229165"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..639
                     /locus_tag="PKU75_09150"
     CDS             154..639
                     /locus_tag="PKU75_09150"
                     /inference="COORDINATES: protein motif:HMM:NF018998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 family protein"
                     /protein_id="HPF81084.1"
                     /translation="MQGTFEGSQVAEFAGQHRHGRCRKVVQGPLHLLDQERVDAHRAA
                     HDDHVGVDDLGDRRRRLAQRPPGLRHCRSRRRVAGLGHREQGQGPGDGVQDQKGWGNV
                     MYIPAAPYCAKNAEYAARCGQSFLAGTSPTDVPPEDYEVGRAGRPTAADLSETGAACW
                     A"
     gene            complement(1032..1319)
                     /locus_tag="PKU75_09155"
     CDS             complement(1032..1319)
                     /locus_tag="PKU75_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81085.1"
                     /translation="MGRLILPTLVLLGILAAAVLLSQGRGGGAPGPGSDSNVLGGDPD
                     GARAIQDARAPAPPSPRQEAWRWTLTVLGILIVSAAVILGIALIAKSLWDL"
     gene            complement(1330..2409)
                     /locus_tag="PKU75_09160"
     CDS             complement(1330..2409)
                     /locus_tag="PKU75_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPF81086.1"
                     /translation="MAKPNTTPTIFPTGDNGGSNSRGSAAGRITGLLKTAVVLGVLAA
                     IVIGLLSVVRTVTSPADEVAVHKGGGVIEAPNSKGCVSANSREIRRPGDKYFWYPASQ
                     RTYNFTGGDGADHAAFDVVSKDYQPLDVPGSVEFTLNVDCEVLTRFHDSIGNRYAAYY
                     TSSDEWDDTPRGWQTMLDLYFAPALDATLDRVAKKYTWKELYADPTIKDEMNKAVNEQ
                     LAALIQQKMPGEDEFFKGFSALIQQPQPADTLVQALGDEEAAKAQAAAAKAKAVADAE
                     TQQAKAIAEAEAAKQAAQAQVAVKEAEAKIAKLEAQIREQEIEPFGSVKEYNNWLAIQ
                     QGLNPYQPTYGGSVVAPAPSSSPSP"
     gene            2577..3458
                     /locus_tag="PKU75_09165"
     CDS             2577..3458
                     /locus_tag="PKU75_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPF81087.1"
                     /translation="MEFTDLPDAASLAAALRATGYVCDDELATIGWLAIRLGRPLLIE
                     GEPGTGKTALAEALSEGLDLPLIRLQCYEGIDAAQALYDWDFPRQILHLRAVESVGGQ
                     GDLAQVEAGLFDERFLVRRPILQALTSSPCVLLIDEIDRADDEFEAFLLEVLSTWQVT
                     IPELGTVRAAVPPLVVLTSNRTRELHDALKRRCLYEWIDHPSLHRELEIVRSRAPEVS
                     AELADQLVRTVQAVRVEPGLIKPPGVAETLDWARALHELGGRLDLETAAATLGVAVKH
                     REDTQRLRAAIDRLLAK"
     gene            3455..4570
                     /locus_tag="PKU75_09170"
     CDS             3455..4570
                     /locus_tag="PKU75_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF81088.1"
                     /translation="MTSGPTLPQHPADTVLLAFARAVRAAGVRVTADRERLYLEAVRT
                     AGLGDPYAVLVAGRATLCASPQDRVRHDQVYEAWFGGEYARMLPSRPPVHTKPMAQLD
                     TADGQGDSGDDDAVSAATSRTEVLRHRDIAQLTLREREALARMFGGIPVRAPVRLSPR
                     LRRARRGRIDAGATLREQVRRLGEPGPIRHRRRAPRPRRIILLVDVSGSMSGYADALL
                     RLAHRIVQSGSPVEVFTVGTRLTHITRALQLRDPDRALIAAGEVVPDWSGGTRLGEML
                     AVFLQTWGRRGPLRGAVVVVFSDGWERDRPEELGEQMRRLHSLAHRVIWVNPHRGKTG
                     YEPIQAGMAAALPHIDDFLAGHSLATFTDLTEVIAHA"
     gene            4563..5711
                     /locus_tag="PKU75_09175"
     CDS             4563..5711
                     /locus_tag="PKU75_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HPF81089.1"
                     /translation="MRDVLNQLSDRFARAETTALATVVATFKSAPRQAGAAMLVGSDD
                     TVVGSVSGGCVEGAVYELGQQVIASGRPVLQRYGISDDDAFTVGLTCGGILDVFVESV
                     SPASFPELPEIVRLVEAEQPVAVATIVAHPDPALVGRRLVIEPSEPHEAGQTVHTAST
                     LGSARADAAVREDALGLLASGRSDTLEYGPDGERRGEGMRVFVNSFAPKPRMFVFGAI
                     DFAAAVARIGSFLGYRVTVCDARPVFATTTRFPDADEVVVDWPHRYLAAQVETGAVGP
                     RSVICVLTHDPKFDVPVLEIALRLDVAYVGAMGSRRTHDDRLARLREVGLGDAELARL
                     SSPIGLDLGARTPEETAVSIAAEIIALQWGGAGERLRATGGPIHHTTP"
     gene            5900..11410
                     /locus_tag="PKU75_09180"
     CDS             5900..11410
                     /locus_tag="PKU75_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPF81090.1"
                     /translation="MFSRIAIVNRGEAAMRLIHAVRDLNAAAAADRQMSVIALHTEGE
                     KGAMFVREADVAYDLGPASARPYLDYAVLERALVDTRADAAWVGWGFVAEDPNFADLC
                     ARLGVTFVGPSAEAMRKLGDKIGSKLIAEEVGVPVAPWSRGGVDSLEDAQEAAARIGY
                     PLMLKATAGGGGRGIRKVTSDAELAAAYQLTRDEAQRAFGSGIVFLEKLITGARHVEV
                     QVIADGQGTAWAIGVRDCSVQRRNQKVIEESASVVMTPEQALVLKESAERLAIAVGYA
                     GAGTVEFLFQPSDGTFAFLEVNTRLQVEHPITEVTTDTDLVKLQLHVASGGRLTGAKP
                     VERGHAVEARLNAEDPDRDFAPAPGRIAHLEFPAGPGIRVDTGVGEGDSIPADFDSMI
                     AKIIAYGRDRDEALARLRRAMRETAVVIEGGATNKSFVLDLLDQPEVIDGSADTGWID
                     RVRADGRLVSAAHSAVALIAAGIEAYDDQEVIERARLLDTARGGRPQVQHQVGAPVEL
                     VLRGAPVKVTTFRLGPNLFRSVIQSGEQVLAAEASLTRWGEYAARLTVNDTAYRLVTA
                     THGPMHLVEVDGVTHRISRDEGGVLRAPAPALVVATPAGVGDEVAAGAPVLVLESMKM
                     ETVVPAPYSGRVKEVLVKAGTQVETGAALVKLEPVGDGDDAVEAAVLDLSLPQPVARD
                     NGAAGLHRAHDVLEGMLLGFDIEGPAAATALHEYLGARDRLVGEGESPLLDDVDLLRI
                     LADFAELSRNRPADGEAHDEMRIHSPREHFQSYLRTLDVERAGLPATFRERLARVLGH
                     YDLPGDLAAPERTPALESAVFRIFLAQQRSAPETKMATALLQRWLGEPAPDGDLAAPI
                     REVLDRLVVATQLRFPIVGDLARSVRFRWFDQPAVDADRSVTLEAVGPELIALDALPE
                     GPERTARLEALADIPERIVAFLGDRLRSGTPRSEPMLAVLIRRHYREHELTDLVEYAV
                     DGRPFAAANYQLDRRPTHLITTIGRLEELRADGPLTRALTHEVESAIARDDRQIALDL
                     YVHAPDLPADPDAASAALSAVFAELPFTPRVRRIAIGVVREAGVEVGYVTLRPQPDGT
                     LVEERPVRDVHPMVGRRLNLWRLRDFTITRLEAPADVLLLHCVGVDNPDDQRLVALAQ
                     VRQLTLVRDEAGKVSGLPHVERALAQCLDGIRHARAALGTREHRLDMNHVWVHIWPPV
                     DADIDQLTALQSKIAPMTADAGIEEVRIEGRLAAAGGRTVPIVARFTYQPGSGVDFTV
                     EPPATTRLAALDAYAEKVVRARRRGLVYPYELQTFIAGDSGTVTELDLDASGGLAPVD
                     RPFGQNRAGIIAALVTTSTELHPEGIERVVLCGDPLRSLGSVAEAECARVMAALDMAE
                     ERRVPVEWFALSAGARIAMDSGTENMDWVAKALRRIVEFTQAGGEINVVVAGINVGAQ
                     PYWNAEATMLMHTKGILVMTPDSAMVLTGKQSLDFSGGVSAEDNFGIGGYDRVMGPNG
                     QAQYWARDLAGALDILLAHYDATYVAAGEDGPRHAETSDPVDRDVTAYPHQLDGSDFA
                     TVGDIWSAASNPDRKKPFDIRTVMRAVADQDHELLERWAGMADAETAVVTDARLGGHS
                     VCLIGIESRTVARQGFPPTDGPDTYTAGTLFPRSSKKVARAINAASGNRPVVVLANLS
                     GFDGSPDSMRNLQLEYGAEIGRAIVNFDGPIVFVVISRYHGGAFVVFSKALNPRMTVL
                     AIEGSFASVIGGAPAAAVVFSRDVDARTAADPRVADLETRLAAATNGQRSVIAAELAD
                     VRSTVRAEMVNAVAAEFDGVHNIHRAVAVGSVDEVIPPASLRSRIITALDAG"
     gene            12305..12799
                     /locus_tag="PKU75_09185"
     CDS             12305..12799
                     /locus_tag="PKU75_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPF81091.1"
                     /translation="MTRITVTVDGDTYTDDVEPRTLLVQYLRENLGKVGTVIGCDTSN
                     CGACTVHLDGRSVKSCNVLAVQADGHEVTTIEGLATDGQLHPMQKAFHENHALQCGYC
                     TPGMIMQAIDILKDHPDPSEQQIREGLEGNLCRCTGYHNIVKAVAAAAKAPGAELASS
                     KVGE"
BASE COUNT         1896 a         4572 c         4529 g         1957 t
ORIGIN      
        1 cggactgtaa atccgtcgcg aaagctacga aggttcgaat ccttcacctg ccacccgtgc
       61 gaagggcctc tcgtccatgg cgaggggccc ttgcctatga ggccggcgtg agccgggtcg
      121 cgatgagaag ctcccgagcg tggctgaggc caggtgcagg gcaccttcga ggggtcccag
      181 gtcgccgagt tcgctggtca gcaccggcat ggacgctgcc gcaaggtcgt ccagggtccg
      241 ctgcacctgc tcgaccagga gcgggtggac gcccaccggg ccgcccatga cgatcacgtc
      301 ggggtcgatg acctcggcga tcgccgtcgc cgcctcgccc agcgcccgcc cggccttcgc
      361 cactgccgca gccgccgccg ggtcgccggc ctcggccatc gagagcaggg ccagggcccg
      421 ggtgatggtg tgcaggacca gaagggctgg ggcaatgtca tgtatatccc ggccgcgccg
      481 tactgcgcaa agaacgccga gtatgccgcc cgctgcggcc agtccttcct cgccggcacc
      541 tccccgacgg acgtcccgcc ggaggactac gaggtgggcc gggccggccg ccccacggcc
      601 gcggacctga gcgagacggg cgccgcctgc tgggcctgat cgactgacga agccaccacg
      661 tatcacgccg gccgcgggct gctcctctcc cacaggagga ttcaccgagg agaacgccat
      721 gtgggagtac gacgagcaac cggcgcgggt gctgagccat gacctgccgt gtctggagtg
      781 tgggcacgcc gcgcaccggt tcctgccatg tgacgccgcc tgcgagtgcg cgtcgtcgcc
      841 cgtccccggg tggtatgacg cctgaccgag tgtgtgtcac ccccgagtgg ggccggtctt
      901 tcatgtgcgg gccgggatat agcccggccc gcgaacgaga gcctggcccg gctttcgaga
      961 agtccgcccg tcaacgagaa cccggcccgg ctttcaagaa ccctggccgg ctcgctgggt
     1021 tgtcagggag gtcagagatc ccagaggctc ttggcgatca gggcgatgcc gagaatcacg
     1081 gcggcactga cgatgaggat gcccaggacc gtcagcgtcc agcgccacgc ctcctgacgt
     1141 ggcgagggcg gcgcgggggc tcgggcgtcc tggatcgcgc gcgctccgtc cgggtcaccg
     1201 ccgagcacat tcgagtccga ccccggcccg ggcgcacccc cgccgcgacc ctgcgagagc
     1261 aggaccgcgg cggcgaggat ccccaggagg accagggttg gcaggatcag ccggcccacg
     1321 cggcatacct cacggactcg gggaggacga cggcgccggc gcgacgacac tgccgccata
     1381 cgtcggctga tacgggttga ggccctgctg gatcgccagc cagttgttgt actccttgac
     1441 gctgccgaac ggctcgatct cctgctcgcg gatctgcgcc tcgagcttgg cgatcttggc
     1501 ctcggcctcc ttcaccgcga cctgcgcctg cgcggcctgc ttcgccgcct cggcctcggc
     1561 gatcgccttg gcctgctggg tctcggcgtc ggccacggcc ttcgccttgg ccgccgcggc
     1621 ctgcgccttg gcagcctcct cgtcaccgag cgcctgcacg agggtgtccg ccggctgcgg
     1681 ctgctggatc agcgcggaga agcccttgaa gaactcgtcc tcgccgggca tcttctgctg
     1741 gatcagcgcg gcgagctgct cgttgaccgc cttgttcatc tcgtccttga tcgtggggtc
     1801 ggcatacagc tccttccacg tgtacttctt cgccacccgg tccagcgtcg cgtcgagcgc
     1861 gggcgcgaag tagaggtcga gcatcgtctg ccagccgcgc ggggtgtcgt cccactcgtc
     1921 ggaggaggtg tagtaggcgg cataccggtt gccgatcgag tcgtggaagc gggtcagcac
     1981 ctcacagtcg acattgaggg tgaactcgac cgagccgggc acgtccagcg gctggtagtc
     2041 cttgctcacc acatcgaacg cggcgtggtc ggcgccatcg cccccggtga agttgtaggt
     2101 gcgctgggac gcggggtacc agaagtactt gtcgcccggc cggcggatct cgcgggagtt
     2161 cgccgacacg cagcccttgg agttcggggc ctcgatcaca ccgccgccct tgtggacggc
     2221 gacctcgtcg gccggcgagg tgacggtgcg caccacgctg aggagaccga tgacgatggc
     2281 agcgaggacg ccgaggacga cggcggtctt gagcagtccg gtgatgcggc cggccgcgct
     2341 gccgcgggag ttcgagccgc cgttgtcgcc ggtcgggaag atggtggggg tggtattcgg
     2401 cttggccatc ggcctgggct ctccttgttc gtccggcaga cgccgagggt atgccgtgcg
     2461 ctcccctcga gcgccgcccc cgcgtcggtc agttctgcga cgcagtgtgc cagggtccgg
     2521 ttccctgccc caagcccgga ccccgttcgg ggcctcggat acggaatgct ggggcgatgg
     2581 agttcaccga cctgcccgac gccgcctcgc tcgccgctgc cctgcgggcg accgggtatg
     2641 tctgcgacga cgagctggcg acgatcggct ggctggcgat ccggctgggc cggccgctgc
     2701 tcatcgaggg tgagccgggg acgggcaaga ccgcgctggc tgaggcgctc tcagagggct
     2761 tggacctgcc actgatccgg ctgcagtgct acgagggcat cgacgccgcg caggccctct
     2821 atgactggga cttcccgcgg cagatcctgc atctgcgagc cgtcgaaagc gttggtgggc
     2881 aaggggatct ggcgcaggtc gaggcgggcc tcttcgacga gcgcttcctc gtgcggcggc
     2941 cgatcctgca ggcgctcacg tcctccccgt gcgtgctgct gatcgacgag atcgaccgcg
     3001 ctgacgacga gttcgaggcc ttcctgctgg aggtgctctc gacctggcag gtgacgatcc
     3061 cggagctcgg cacggtgcgg gctgcggtgc ccccgctcgt cgtcctgacc tccaaccgca
     3121 cgcgcgaact gcacgacgcc ctcaagcgcc gctgcctgta tgagtggatc gaccacccct
     3181 cgctgcaccg cgagctggag atcgtgcgca gccgggcgcc ggaggtcagc gccgagctgg
     3241 ccgaccagtt ggtgcggacc gtgcaggcgg tgcgggtcga gccggggctg atcaagccgc
     3301 cgggggtggc cgagacgctc gactgggcgc gggcgctgca cgaactcggt ggccgactcg
     3361 acctcgagac ggcggcggcg accctcgggg tcgcggtcaa gcaccgtgag gacacgcaac
     3421 gcctgcgggc cgcgattgat cggctgctcg cgaaatgact tctggcccaa cgcttccgca
     3481 gcatccggcc gatacggtgc tgctggcgtt cgcgcgcgcg gtgcgcgctg ccggggtgcg
     3541 ggtgacggcg gaccgtgagc ggctctacct cgaggcggtc cgcaccgccg ggctcggcga
     3601 tccgtatgcc gtgctcgtcg ccggccgcgc caccctctgc gcctcgccgc aggaccgggt
     3661 gcggcacgac caggtgtatg aggcgtggtt cggcggcgag tatgcccgca tgctcccctc
     3721 ccgcccgccg gtgcacacca aaccgatggc ccagctggat accgctgatg ggcagggaga
     3781 ttcgggcgat gacgacgcgg tcagcgccgc gacgagccgg accgaggtgc tgcgccatcg
     3841 cgatatcgcc cagttgacct tgcgggagcg ggaggcgctg gcgcggatgt tcggcggcat
     3901 acccgtgcgg gcgccggtgc ggctgtcgcc gcggctgcgg cgggcccggc gggggcgcat
     3961 cgacgccggc gcgaccttgc gcgagcaggt gcgccggctg ggggagcccg ggccgatccg
     4021 gcatcgccgc cgggcaccgc ggccacggcg gatcatcctg ctcgtcgacg tcagcgggtc
     4081 gatgagcggc tacgccgacg ccttgttgcg cctggcccac cgcatcgtcc aatccggttc
     4141 tcctgtggag gttttcaccg tcgggacccg gctcacacac ataactcggg cgctccagct
     4201 gcgcgacccg gaccgggcgc tgatcgccgc cggagaagtc gtgcccgact ggtcgggcgg
     4261 cacgcgcctg ggggagatgc ttgcggtctt cctgcagacg tggggcaggc gcgggccgct
     4321 gcgtggagcg gtggtcgtcg tcttcagcga cgggtgggag cgggatcggc ccgaggagct
     4381 gggggagcag atgcggcggc tgcactctct cgcgcaccgg gtgatctggg tcaatccgca
     4441 ccgcggcaag accggctacg aaccgatcca ggcggggatg gccgccgcgc tgccccatat
     4501 cgacgacttc ctcgccggcc actcgctggc gaccttcacc gatctgacgg aggtgatcgc
     4561 gcatgcgtga cgttctcaat cagttgtcgg accgttttgc tcgtgccgag acgacggcgc
     4621 tggcgacggt ggtggcgacc ttcaagtcgg cgccccgcca ggcgggggca gcgatgctcg
     4681 tcggctcgga cgacaccgtc gtcgggtcgg tgtccggcgg ctgtgtcgag ggcgccgtct
     4741 acgagctggg tcagcaggtc atcgcctccg gccgaccggt cctgcagcgc tatggcatca
     4801 gcgacgacga cgcgttcacg gtcgggctga cgtgcggcgg catcctcgac gtcttcgtcg
     4861 aatcagtctc tccggcaagc tttcccgagc tgcccgagat cgttcggctc gtcgaggcgg
     4921 agcagccggt ggcggtggcg acgatcgtcg cccaccccga cccggcgctc gtgggccggc
     4981 gactcgtgat cgagccgagc gaaccccacg aggcgggcca gactgtccat accgcctcca
     5041 cgctgggctc ggcgcgggcc gacgccgcgg ttcgcgagga cgccctcggc ctgctcgcgt
     5101 ccgggcggtc cgacacgctc gaatacgggc cggacggcga gcggcggggc gagggcatgc
     5161 gcgtcttcgt caactcgttc gcgccgaagc cgcggatgtt cgtcttcggg gcgatcgact
     5221 tcgccgccgc cgtggcgcgc atcggctcct tcctcggcta tcgcgtcacg gtctgcgacg
     5281 cccgcccggt cttcgcgaca acgacccggt tccccgacgc cgacgaggtc gtcgtcgact
     5341 ggccgcaccg ttatctggcg gcgcaggtcg agaccggagc cgtcgggccg cggagcgtca
     5401 tctgcgtgct gacgcacgat ccgaagttcg acgtgcccgt gctggagatc gcgctccggc
     5461 tcgacgtggc gtatgtcggt gccatgggct cgcgccgcac ccacgacgac cggctggcgc
     5521 gcctgcggga ggtcggactc ggcgatgccg agctggcccg gctgtcgagc ccgatcggcc
     5581 tcgacctcgg cgcccgcacc cccgaggaga cggccgtctc cattgccgcc gagatcatcg
     5641 ccctgcagtg gggcggagcc ggcgagcgcc tccgtgccac cggcggcccc atccaccaca
     5701 cgacgccgtg atctcctcga caagaaaacc cagtcgagag agcgagacac gcccaggaaa
     5761 cggccggatt gtgggcgtgt ctcgctcttt cgacttccgg acgttgggta ttggtggcct
     5821 aagcgctcgc ataacggctg ggtgtgacct ggcccactag ggttattggg ttgccgtcgc
     5881 ctccgccgaa gggtcgttga tgttctcgcg catcgccatc gtcaatcgcg gcgaggccgc
     5941 tatgcgtttg atccacgcgg tgcgcgacct gaacgccgcg gctgcggcgg accggcagat
     6001 gagtgtcatc gcgctgcaca ccgagggcga gaagggcgcg atgttcgtcc gggaggcaga
     6061 cgtcgcctat gacctcggcc cggcgagcgc gcggccctac ctcgactacg cggtcctgga
     6121 gcgagccctg gtcgacacgc gggccgatgc ggcctgggtc gggtgggggt tcgtcgcgga
     6181 agacccgaac ttcgccgacc tgtgcgcccg gctcggtgtc accttcgtcg gccccagcgc
     6241 cgaggcgatg cgcaagctgg gcgacaagat cggcagcaag ctgatcgcgg aggaggtcgg
     6301 ggtcccggtc gcgccgtgga gtcgcggcgg ggtcgacagc ctggaggatg cccaggaggc
     6361 ggccgcgcgc atcggctacc cgctcatgct caaggcgacc gcgggcggcg gtggccgtgg
     6421 catccgcaag gtgacgtccg acgcggagtt ggccgcggca taccagctga cgcgggatga
     6481 ggcgcagcgc gccttcggct cgggaatcgt cttcctggag aagctgatca ccggagcgcg
     6541 acacgtcgag gtgcaggtga tcgccgacgg gcagggcacg gcgtgggcca tcggtgtgcg
     6601 cgactgctcg gtgcagcggc gcaaccagaa ggtcatcgag gaatccgcgt ccgtcgtcat
     6661 gacgcccgag caggcgctcg tgctgaagga gagcgcagag cggctcgcca tcgcggtcgg
     6721 gtatgccggc gccggcaccg tcgagttcct cttccagccg tccgacggca ccttcgcctt
     6781 cctcgaagtc aacacccggc tgcaggtcga gcacccgatc accgaggtca ccaccgacac
     6841 cgatctggtc aagctccaac tgcacgtggc gagcggcggg cggttgacag gggcgaagcc
     6901 cgtcgagagg gggcacgccg tcgaggcccg tctgaacgcc gaggatccgg accgtgactt
     6961 cgcgccggca ccgggccgca tcgcgcacct ggagttcccg gccggccccg gcatccgggt
     7021 ggacaccggt gtgggggaag gggattcgat ccctgcggac ttcgattcga tgatcgccaa
     7081 gatcatcgcc tacggccgcg accgggacga ggcgctggcc cggctgcggc gcgccatgcg
     7141 cgagaccgcc gtcgtcatcg agggcggggc gaccaacaag tccttcgtcc tcgacctgct
     7201 cgaccagccg gaagtcatcg acggcagcgc cgacaccggc tggatcgacc gcgtgcgcgc
     7261 cgacggccgg ctcgtgagcg cggcccactc cgcagtggcc ctgatcgccg ccggcatcga
     7321 ggcgtatgac gaccaggagg tcatcgagcg ggcccgcctc ctcgacacgg cacggggcgg
     7381 tcggccgcag gtgcagcatc aggtgggcgc accggtcgag ctggtgctgc ggggcgcgcc
     7441 cgtcaaggtg acgacgttcc ggttggggcc caacctgttt cggtccgtca tccagtccgg
     7501 cgagcaggtc cttgcggccg aggcgagcct gacgcggtgg ggggagtatg ccgcccgcct
     7561 caccgtcaac gacacggcct accggctcgt gacggcgacg cacggaccga tgcatctcgt
     7621 cgaagtcgac ggcgtcacgc atcggatcag ccgcgacgag ggcggcgtcc tgcgggcacc
     7681 cgcaccggcc ctcgtcgtcg ccacacccgc cggcgtcggt gacgaggtcg ccgccggcgc
     7741 accggtgctc gtgctcgaat cgatgaagat ggagaccgtc gtcccggctc cttactcggg
     7801 ccgggtcaag gaggtgctcg tcaaggccgg cacccaggtc gagaccgggg ccgccctcgt
     7861 gaaactcgag ccggtcggcg acggtgacga cgcggtcgag gcggccgtgc tcgacctgtc
     7921 cctcccgcag cccgttgccc gggataacgg tgccgcgggc ctgcaccggg cccacgacgt
     7981 gctcgaaggg atgctcctgg ggttcgacat cgagggtccg gcggccgcca ccgccctgca
     8041 cgaatacctg ggcgcccggg atcgccttgt cggggaaggc gaatcgccgc tcctcgacga
     8101 tgtcgacctg ttgcggatcc ttgccgattt cgccgagctg agccgcaacc ggcccgccga
     8161 cggcgaagcc cacgacgaaa tgcggatcca ctcgccgcgc gagcacttcc agagctatct
     8221 gcgcaccttg gacgtcgaac gcgccgggct gccggccacc ttccgcgaac gcctggcccg
     8281 ggtgctcggg cattacgacc tgcccggcga tctcgccgcc cccgaacgca cgccggcgct
     8341 ggagtcggcc gtcttccgga tcttcctggc gcagcaacgt tccgcccccg agaccaagat
     8401 ggcgaccgct ctgttgcagc gatggctcgg cgaacccgca ccggatggtg acctcgcggc
     8461 gccgatccgc gaggtgctcg accggctcgt cgtcgcgacc caattgcgtt tcccgatcgt
     8521 cggtgacctg gcccgcagcg tgcgcttccg ctggttcgac caacccgccg tcgacgccga
     8581 ccgttcggtc acactcgaag cggtcgggcc cgaactcatc gcgctggacg ccctgcccga
     8641 gggccccgag cgcacggccc ggctggaggc gctggccgac atccccgaga ggatcgtggc
     8701 ttttctcggc gaccggttgc gttccgggac accgcgatcg gagccgatgc tcgctgtgct
     8761 gatccggcgc cactatcgcg agcacgagct gacggatctc gtcgagtatg ccgtcgacgg
     8821 acgcccgttc gccgccgcga actatcagct cgaccggcgc cccacgcacc tgatcacgac
     8881 gatcgggcgg ctcgaagaac tccgcgcgga cgggcccctg actcgcgccc tcacccacga
     8941 agtcgagtcg gcgatcgccc gggacgaccg gcagatcgcc ctcgacctct atgtccacgc
     9001 gccggacctt ccggcggatc cggacgccgc gtccgccgcc ctctccgcgg tcttcgccga
     9061 gctgcccttc acccctcgcg tgcgcaggat cgcgatcggc gtcgtgcgcg aggcgggcgt
     9121 cgaggtgggt tatgtgacct tgcgcccgca accggacggg accctcgtcg aagaacgtcc
     9181 ggttcgcgac gtccacccga tggtcgggcg gcgcctcaac ctgtggcggc tgcgggactt
     9241 caccatcacc cgcctcgagg caccggccga cgtgctcctg ttgcactgcg tcggtgtcga
     9301 caaccccgac gaccaacgcc tcgtcgccct cgcccaggtc cgccagctca cgctggtgcg
     9361 ggacgaggca ggcaaggtgt cgggcctccc gcacgtcgag cgcgccctgg cacagtgtct
     9421 cgacggcatc cggcacgccc gcgccgccct cggcacgcgc gagcaccggc tggacatgaa
     9481 ccacgtctgg gtgcacatct ggccgccggt ggacgccgac atcgaccagc tcacggccct
     9541 gcagagcaag atcgcgccga tgacggccga cgcgggcatc gaggaggtgc gaatcgaggg
     9601 ccggctcgcc gccgccggcg gccgcaccgt cccgatcgtc gcgcgcttca cctatcaacc
     9661 gggttcgggc gtcgacttca cggtcgaacc gccggcgacg acgcggctgg ccgctctcga
     9721 tgcgtatgcc gagaaggtcg tccgcgcccg ccgccgcggc ctggtctatc cctacgagct
     9781 ccagacgttc atcgcggggg acagcggcac ggtgaccgaa ctcgatctcg atgcgtccgg
     9841 cggcctggcc ccggtcgacc ggccgttcgg gcagaaccgc gcgggcatca tcgccgccct
     9901 cgtgacgacg tcgaccgaac tgcaccccga gggcatcgaa cgtgttgtgc tgtgcggaga
     9961 tccgctgcgc tcgctcggat ccgtcgccga ggcggagtgt gcccgggtca tggccgcgct
    10021 ggacatggca gaggaacgac gcgtcccggt cgagtggttc gccctgtccg ccggggcgcg
    10081 gatcgcgatg gactccggca ccgagaacat ggactgggtc gccaaggcgc tgcggcggat
    10141 cgtcgagttc acccaggccg ggggtgagat caacgtcgtc gtcgcgggca tcaacgttgg
    10201 ggcgcaacct tattggaacg ccgaggcgac gatgctcatg cacaccaagg gcatcctcgt
    10261 catgaccccc gactcggcca tggtcctgac cggcaagcag tcgctggact tctcgggcgg
    10321 cgtcagtgcc gaggacaact tcggcatcgg tggctatgac cgtgtcatgg gtccgaacgg
    10381 tcaggcgcag tactgggcgc gcgatctcgc gggcgccctc gacatcctcc tcgcccacta
    10441 tgacgcgacc tatgtcgccg cgggtgagga cggtccgcgg cacgcggaga cgagcgaccc
    10501 ggtcgaccgc gacgtcacgg catacccgca tcaactcgac gggagcgact tcgcgacggt
    10561 cggcgacatc tggtccgccg cgtcgaaccc ggaccgcaag aagcccttcg acatccgcac
    10621 cgtcatgcga gctgtggcgg atcaggacca cgagctgctc gagcggtggg cgggcatggc
    10681 cgatgccgag acggcggtgg tcactgacgc gcggctgggc ggccactcgg tgtgcttgat
    10741 cggcatcgag tcgagaaccg ttgcgcgcca agggttcccg cccacggacg ggccggacac
    10801 ctacacggcc ggcaccctct tcccacgctc gtccaagaag gtcgcccggg caatcaacgc
    10861 cgcctcgggc aaccggccgg tggtcgtcct ggcgaacctg tccggcttcg acgggtcacc
    10921 ggactccatg cgcaacctgc agctcgaata cggcgccgag atcggccggg ccatcgtcaa
    10981 cttcgacggg ccgatcgtct tcgtcgtcat ctcccgctat cacggcggcg cgttcgtggt
    11041 cttctccaag gcgctcaatc cccgcatgac ggtgcttgcg atcgagggct cgttcgcctc
    11101 ggtgatcggc ggggcacccg ctgccgcagt ggttttcagc cgcgacgtcg acgcccgcac
    11161 cgccgccgac ccccgtgtcg ccgacctcga gacccgcctc gccgccgcca ccaacggaca
    11221 gcgcagcgtg attgcggccg aactcgccga cgtgcgctcc acagtccgcg ccgagatggt
    11281 caacgccgtc gccgccgagt tcgacggcgt gcacaacatc caccgcgccg tcgccgtcgg
    11341 ctccgtcgac gaggtcatcc ccccggcgtc cctgcgctcc cgcatcatca ccgccctcga
    11401 cgccggttag gtcgtcgccg ggcctggacc tcacgccgtg ctctggggag cggcctggag
    11461 cggaattgcc attccgctga cgtgggaaag atcccgcagt acgctcccct cgccgtcggt
    11521 gccgtgctgg gcccgatccc tgggcgaccc tcgcggccgg ttcgcgctgg cggcgcgact
    11581 tccgcatttc gtgtccgaac cgggaggagg tgcgtgatgc tcagtcggtt catggcgcag
    11641 acacgaactt gttgttgtcg gctggttcga ggtgacatgt cgcgactcgt gatccttgcc
    11701 ctgggcacgc caagcagccc agcggcttta cgggttgtgc gcgtccgctt cgccgatggt
    11761 cgcgaccacc gaacgatccg cggggcagac ccagaatgcc aacgagtcaa cgacggccgc
    11821 ctgtagcgga tgacgagcgg ggtgagccgg gcagggcggc caggtggtct gaccgagctc
    11881 ccacagctga ccctcgatga ccacctcctg gacctgatcc gcggccgagg cgacccggtc
    11941 cgcgacgggt gcatatcggt cgaccgccag ccccgtcgcg ctgccatcgg cgtcccgcat
    12001 catgcacgca gcccgatcga tcgtccgccc agtcatggtc atcgaacctg aggcccgtag
    12061 acagctccgt tcaatgtcac gcaagaccgg ctcgactgat tcggtaagga cacggtccat
    12121 agggcgagcg tgccccactc ccggccgcgt ttgctggccg acggtggtcg gcgaggttcg
    12181 caggcatacc gcgacgtgac gcccacggaa tccgcgagtg tgggccttgt cacctcggcg
    12241 cgtctccgcc aggatcatcg gtgaccgacg agccaggcac atcgggccac gaaggagtga
    12301 ccgcatgacg cgcattaccg tcaccgtgga cggggacacc tataccgacg acgtcgagcc
    12361 acggaccctg ctggtccaat acctgcgcga gaatctcggc aaggtcggca cggtcatcgg
    12421 ctgcgacacg agcaactgcg gggcctgcac cgtccacctg gacgggcgca gcgtgaagtc
    12481 ctgcaacgtc ctggccgtgc aggccgacgg gcacgaggtc acgaccatcg agggcctcgc
    12541 caccgacggc caactgcacc cgatgcaaaa ggccttccac gagaaccacg ccctgcagtg
    12601 cggctactgc acgcccggca tgatcatgca ggccatcgac atcctcaagg accaccccga
    12661 cccgagcgag cagcagatcc gcgagggcct cgagggcaac ctgtgccgct gcaccggcta
    12721 ccacaacatc gtcaaggccg tcgcggccgc cgccaaggcg cccggcgccg aactcgcaag
    12781 cagcaaggtg ggtgagtgac atgaccgtca gccccgacgc cctcgtcgag atcgccgagg
    12841 agagcccgca ggccgacctg accgagccca gcggggcggg acccgagatc ggcaaccgcc
    12901 ggctgcgcaa ggaggaccag cgcctcatca ccggccgaac caagtggacc gaca
//
LOCUS       DAOPJG010000087        12913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5099_length_12913_cov_7.125992, whole genome shotgun sequence.
ACCESSION   DAOPJG010000087 DAOPJG010000000
VERSION     DAOPJG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12913
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5099_length_12913_cov_7.125992"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..750
                     /locus_tag="PKU75_09190"
     CDS             139..750
                     /locus_tag="PKU75_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81092.1"
                     /translation="MAQIEAGPVAYRRWLFVTACAYAVLHHLGLLPAGTARWRGTSWV
                     DWLDLVVPYAALAPAALTLATARVTGRHWGVFAVGALAYTQGHGIHLAANSIGNADPG
                     ETAYLWDELAGHAIWYAGAAVVMFVLAATMARESPRAHPLGVLAALGVGATNALGGHT
                     IPLAIVVALAGIGWGWTRRHTVAWLTGIAGAAALVVLAGAALV"
     gene            complement(747..2216)
                     /gene="der"
                     /locus_tag="PKU75_09195"
     CDS             complement(747..2216)
                     /gene="der"
                     /locus_tag="PKU75_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPF81093.1"
                     /translation="MTTTPPPPETDGDDIALDRALRVGLDEFDLTEEDRALLDIEGFE
                     DEQRTEVVLPVVAIVGRPNVGKSTLVNRILRRREAVVEDVPGVTRDRVAYEAEWSGRR
                     FTIIDTGGWSIDATGIHLRVAEQAEIAVEMADVVLFVVDAKVGATDDDEAVVRMLRRA
                     GKPVILCANKVDDDRFEADAMALWNLGLGEPFAISALHGRGSGDLLDKILELLPEESH
                     VAAAGQEGGPRRVALLGRPNVGKSSLLNKLAGQERVVVDDLAGTTRDPVDELVELGGK
                     PWRFVDTAGIRRRVHQTQGADFYASLRTQAALEKAEVAVVLIDAGTAITEQDIRIVQQ
                     VIDAGRALVIVYNKWDTMDEERRYYLEREIERELVQVPWAPRVNISARTGRHTDKLLP
                     ALETALDSWDQRIPTARLNAFLGEVVAAHPHPVRGGKQPRILFGTQAGTRPPRFVLFA
                     SGFLEAGYRRFLERRLREEFGFEGSPIEVSVRVREKRKR"
     gene            complement(2422..3114)
                     /gene="cmk"
                     /locus_tag="PKU75_09200"
     CDS             complement(2422..3114)
                     /gene="cmk"
                     /locus_tag="PKU75_09200"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPF81094.1"
                     /translation="MVAPVVIAIDGPSGSGKSSVSRAVATHFGFGYLDTGAMYRAVTL
                     WCLRQGIDLTDAGAVVAAAEDLPLGLGADPQDPTVELDGDDIAEAIRTSEVSAEVSKV
                     ATNLGVRAVLQEQQRDLIAIIADDFGGVVAEGRDITTVVAPDADVRILLTASEEARLA
                     RRSTQLHGTADAATVAATHDEVVRRDRDDSTVSQFTVAADGVVLLDTSALDFDGSVAA
                     VIGIVEHAEERE"
     gene            complement(3209..4336)
                     /locus_tag="PKU75_09205"
     CDS             complement(3209..4336)
                     /locus_tag="PKU75_09205"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPF81095.1"
                     /translation="MPRALPPPVLPQRVHIIGSGLIGTSLAMALVRAGLVVSVADASP
                     TNEALASDLSGARIGAGDPVDLVVVATPPDVTADVVTHALATWPNAVVTDVASVKEIV
                     RAELDAKEVPGRERYVGGHPMAGREKSGAIAARIDLFEGRPWVISRTETTDPDGVALV
                     RGIAEAAGAVVRLLSPTEHDAAVAAVSHVPQIAASLVAARLADLSEDAVGLSGTGVRD
                     VTRIAGSDPVLWTQILTGNAGAVAAVLKALAADLGQIVDALDSLAAATTPDGQIEAPG
                     ARAVLARTIAAGNAGQARIPGKHGAAPAAYAVVTVLIPDEPGQLGRLFQDVGDAGINL
                     EELNLEHNLGQPYGQLELSVLPAVAADLTDWLTARGWRVHD"
     gene            complement(4342..5241)
                     /locus_tag="PKU75_09210"
     CDS             complement(4342..5241)
                     /locus_tag="PKU75_09210"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPF81096.1"
                     /translation="MTPPKPRSGGSGRPGRPRGQAGGTPGRRRGPQHAGTTKKSQPTR
                     VPVEPHRDNVDVHDPDGIRLQKLLAASGIGSRRACEQMISEGRVEVDGQIVMELGVRV
                     DPTRQTIHVDGVRVQTDPEKVYLAFNKPVGVVTTMQDEKGRPCVGDYVAGRRERLFHV
                     GRLDADTEGLLLLTNDGELANRLAHPSYEVLKTYVAQVPGPVPRDLGKTLRAGVDLED
                     GRVVVDSFKLVDSMPGKAVVEVILHEGRNRIVRRLLEAVGHPVIELARIQFGPVRLGN
                     TKAGKMRKLTAREISELYDAVGL"
     gene            complement(5238..5885)
                     /gene="scpB"
                     /locus_tag="PKU75_09215"
     CDS             complement(5238..5885)
                     /gene="scpB"
                     /locus_tag="PKU75_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPF81097.1"
                     /translation="MSEHETEPVVSEDLVADESAAEEDGPLVLDVDAMPGGAKAAIEA
                     ILMVTDDPVTSMALATALEIPVPAVEAHLQGLIADYGDRGFTLREVAGGWRIYSRPEY
                     AAAVERFILEGATAKLTQASLETLAVIAYRQPISRGRVGAVRGVNVDGVVRTLLTRGL
                     IEEVGSEPESGAILYGTTDYFLERLGLRDLSELPALAPYLPEVDILDELAETGRA"
     gene            complement(5882..6781)
                     /locus_tag="PKU75_09220"
     CDS             complement(5882..6781)
                     /locus_tag="PKU75_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HPF81098.1"
                     /translation="MTTDVLVGEGDFSGDAVPGGVITRRAPENAFSVHLDNFEGPFDL
                     LLGLIAKHKLDITEIALAQVTDEFLAHLRQLREAGDWDLSQTTEFLLVAATLLDLKAA
                     RLLPQLDPQDEEDLALIEARDLLFARLLQYRAFKQIAHVFEDRMAVNRAMVARRAGVD
                     PRFEKLLPELVIGITPEQLAMIAARAMTPKAVPTVGLDHLHAPAVSVQEQAGIIGERL
                     RHAKVATFRALVSDADSTLVIVARFLALLELYREANVTFEQAEALGELTIRWVGTESG
                     EITVSDEFDESDDAESDEGEDEQ"
     gene            complement(6787..7677)
                     /locus_tag="PKU75_09225"
     CDS             complement(6787..7677)
                     /locus_tag="PKU75_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF81099.1"
                     /translation="MERSDVHLPGTEDAEIGQYGPTGRQLPAFPEPPALTSHGPARVI
                     ALCNQKGGVGKTTTTINLGAALAEYGRKVLLIDFDPQGALSVGLGINSNDLDVTIYNL
                     LIERDHDIREVIQHSRVPGLDVVPANIDLSAAEVQLVGEVAREQVLARVMRPVLDDYD
                     VILIDCQPSLGLLTVNALTAAHGVLIPLETEYFAMRGVALLVETIEKIIDRLNPRLQI
                     DGILATMYDGRTLHSREVVESVVAHFGDRVLHTVISRTVKFPDATLAAEPITSYASAH
                     SGANAYRQLARELIARGSAP"
     gene            complement(7729..8670)
                     /gene="xerD"
                     /locus_tag="PKU75_09230"
     CDS             complement(7729..8670)
                     /gene="xerD"
                     /locus_tag="PKU75_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPF81100.1"
                     /translation="MPDPQRLPDALDRALRDWLTHLDVERGLSRHTLRAYERDVGRYA
                     AYLRSRGITDLAAVDSAVVSDFLVALRSGEGDHPPLAASSAGRAVVAVRAFHRFLALD
                     GALEADPARSVTPPKTPLRLPKAIPLDRVEALLAAASVGDTPAALRDRALLEVLYGVG
                     ARVSEVVDLDVDDLDRDSEVVRLRGKGDKERIVPLGSYAAAALDAYRTRARPVLAAKG
                     RGIPALFLNQRGGRLTRQGVWAILKATAERAGITEEISPHTLRHSFATHLLQGGADVR
                     IVQELLGHASVTTTQIYTKVTIDQLREVYAGAHPRAR"
     gene            complement(8692..9807)
                     /gene="ald"
                     /locus_tag="PKU75_09235"
     CDS             complement(8692..9807)
                     /gene="ald"
                     /locus_tag="PKU75_09235"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPF81101.1"
                     /translation="MKIGVPTEVKNHEYRVGITPVGVHELVGHGHEVLIQKGAGLGSS
                     ITDEEFVSQGARIIDSADDVWGEADMVMKVKEPVKEEYHRLREGLTLFTYLHLAADQP
                     LTDELVATKVTGIAYETVQLPSGMLPLLYPMSEVAGCLAPQVGAHSMMRPYGGRGVLM
                     GGVGGVANAKVVVLGAGVAGQNAANIALGMGADVTLLDTDLDKLRMSFWRYDNRVHQI
                     ASSALAVREQVLQADLVIGTVLVPGAKAPKLVTDEMVAAMKPGSVLVDVAIDQGGCFE
                     GSRPTTHADPTFTVHNSIYYCVANMPGAVPNTSTWALTNATLGYAVKLADKGWQQAMR
                     DDRALAFGLNTHAGEVTCPGVADAFGMECADTAAIVA"
     gene            complement(9915..11717)
                     /locus_tag="PKU75_09240"
     CDS             complement(9915..11717)
                     /locus_tag="PKU75_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HPF81102.1"
                     /translation="MTDNPSTTGAEANDATTEVETPVETSADETSADEVVQAPEPAPE
                     AAPAAEDTPGDLAAEPAIVVEPAPAPAKEEIIEVPDDPDELAPEPEAEPASESESEPE
                     PEPASEPESDPEAEPAGQPESDPETEPASEPEPGAEPAGEAEPEPAAAPVPAPPKPSA
                     APSPKMFAARPKPPAAAPVQAHTPSDSARFGRVAEDGTVFVKDGEGERAVGSYPGATD
                     EEALQYFARKYDELYAAADLLTQRAALPEVTSKDLAEGLTGLRTHIGEANVVGDLVAL
                     TALVDGVESQITSKRESESAARAAAKAEALTVREDLVAQAEAIAGQPVERIQWKTSSA
                     TMRALLDSWKEQQRTGARLDKDVEAALWQRFSRARNSFDKARRTHFAELETTRTAARS
                     SKEKLVKEAQALATSKDWAATAGAFKRLMDQWRQAGRASRAEDDALWEQFKAAQDSFF
                     AAKDAVVAAEDEEYRANLEVKLGLLAEAEKLLPVQDLEATKAALRVIQDKWDAAGKVP
                     RADIDRTEKGMRRVEAAVREADEKKWRRTNPEVTARAQSLATQLEASLAKVEEELAQA
                     QAKGNSTKIADLTAKLEAQRSWLEQARGGLSDIG"
     gene            complement(11721..12557)
                     /locus_tag="PKU75_09245"
     CDS             complement(11721..12557)
                     /locus_tag="PKU75_09245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPF81103.1"
                     /translation="MTKDQERARAKRRYERQQANLAAQQAAQKRNLQVAAVVAAVIAV
                     VAAFLLLSKVMGDTNEQPSAGATGNSASASGSGSASDAPVAAGCDAAPAAPGTGGELD
                     APDAAAIKAVEGKTVVASLATNCGAIELTLDGAKAPKTVASFVQLAQQGYWKDAPCHR
                     LTTAGIYVLQCGDPTGSGSGGPGYGYGIENAPADGKYPAGTVAMARTQDPNSNGGQYF
                     IVYQDSELPTDGGGYTIFGTVTAGMDIVDKIAAGGVADGGSDGAPKFPISTLTVTTET
                     KG"
     gene            12603..>12913
                     /locus_tag="PKU75_09250"
     CDS             12603..>12913
                     /locus_tag="PKU75_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF81104.1"
                     /translation="MFMLAFPAAAFATNCYVIAAAPGEECLVVDPGIGVLPTLQEIFR
                     EHRLRPAAVLLTHGHLDHVYSVTPVCQGETPAVVPAYVHQDDRYRLTDPITYLDPAIV
                     AA"
BASE COUNT         1752 a         4404 c         4656 g         2101 t
ORIGIN      
        1 accccttgac ccccagtcaa gtgcgctacc aaactgcgcc acagcccgtg acaatgcccc
       61 ccgaccggga ggcgaacgca accatacctc acgccctgcc tgccgcccga atcggttgtc
      121 tcgcgcgagg atgtggcggt ggcgcagatc gaggcggggc ccgtggcata ccggcggtgg
      181 cttttcgtca ccgcctgcgc gtatgccgtg ctgcaccacc tcggtctcct gccggcgggc
      241 acggcgcgct ggcgcgggac cagctgggtg gactggctcg acctcgtggt gccgtatgcc
      301 gcgctcgctc ccgccgcgct caccctcgcc accgcgcggg tcaccgggcg gcactggggc
      361 gtcttcgcgg tcggcgcgct ggcctacacc cagggtcacg gcatccacct ggcggcgaac
      421 tcgatcggaa acgcggaccc gggcgagacc gcatacctct gggacgaact ggcggggcac
      481 gcgatctggt atgccggcgc ggcggtcgtc atgttcgtcc tggctgccac gatggcgaga
      541 gagtcaccgc gcgcccaccc gctcggggtg ctggcggctc tcggtgtcgg ggcgacgaac
      601 gcccttggcg gacatacgat tccgttggcg atagtcgtcg ctctggcggg gatcggatgg
      661 ggctggacgc ggcggcatac cgtggcgtgg ctcaccggca tcgctggtgc ggcggcgctg
      721 gtggtgctgg ccggagccgc cctcgtctag cgcttgcgct tctcccgcac gcgcaccgag
      781 acctcgatcg gcgagccttc gaagccgaac tcctcacgca gccggcgctc gaggaagcgg
      841 cggtagccgg cctccaggaa gccggaggcg aagagcacga atcgcggcgg gcgggtgccg
      901 gcctgggtgc cgaagaggat gcgcggttgt ttgccgccgc ggaccggatg cgggtgggcg
      961 gcgacgactt cgccgaggaa ggcgttgagg cgggcggtgg ggatgcgctg gtcccacgag
     1021 tcgagtgcgg tctcgagggc tgggaggagc ttgtcggtat gccggccggt ccgggccgag
     1081 atgttgaccc gcggcgccca ggggacctgg accagttcgc gctcgatctc ccgctcgagg
     1141 tagtagcgcc gctcctcatc catggtgtcc cacttgttgt agacgatgac cagggcgcgg
     1201 ccggcgtcga tgacctgctg gacgatgcgg atgtcctgct cggtgatcgc ggtgccggcg
     1261 tcgatgagga cgacggcgac ctcggccttc tcgagggcgg cctgcgtgcg cagcgaggca
     1321 tagaaatcgg cgccctgggt ctggtggacg cggcgccgta tgccggctgt gtcgacgaat
     1381 cgccacggct tgccaccgag ttcgacgagt tcgtccaccg ggtcgcgggt ggtgccggcg
     1441 agatcgtcga cgacgacccg ctcctgcccc gccagcttgt tcagcaggga ggacttgccg
     1501 acgttcggac gcccgagcag cgcgacccgg cgggggccgc cctcctggcc ggcggcggcg
     1561 acgtgcgact cctccgggag cagctcgaga atcttgtcga gcaggtcgcc ggagccgcgg
     1621 ccgtgcagcg cggagatggc gaacggttca ccgaggccga ggttccacag ggccatggcg
     1681 tcggcttcga accggtcgtc gtcgaccttg ttggcgcaca ggatgaccgg cttgccggcg
     1741 cggcgcagca tgcgcacgac ggcctcgtcg tcgtcggtgg cgccgacctt ggcgtcgacg
     1801 acgaagagca cgacatcggc catctcgacg gcgatctcgg cctgctcggc cacgcgcagg
     1861 tggatgccgg tggcgtcgat gctccagccg ccggtgtcga tgatcgtgaa gcgtcgaccc
     1921 gaccactcgg cctcatacgc gacgcggtcg cgggtgaccc ccggcacgtc ctcgacgacc
     1981 gcctcgcggc ggcgcaggat ccggttgacg agggtggact tgccgacgtt cgggcgcccg
     2041 acgatggcca cgaccggcaa gacgacctcg gtccgctgct cgtcctcgaa gccctcgatg
     2101 tcgaggaggg cgcggtcctc ctcggtcagg tcgaactcgt ccaggccgac gcgcagcgcg
     2161 cggtccaggg cgatgtcgtc cccgtcggtc tcgggcgggg gcggggtggt cgtcatacct
     2221 tccagtgtcc cgtatgcggt gcgcccgccc gaaatcgttg ctgccgcccg cccgcggagg
     2281 tggcgaactg accgggttac cgcgaactgc ctgacatcgc gccaactgcc tgacttcggg
     2341 ccaactgcct gacgtatttc tcaggcagtc cgcgccaccc caggcggctc gcgcttcacg
     2401 aggcagttcg gagcgagcgg gctactcgcg ctcctctgcg tgctcgacaa tgccgatgac
     2461 ggccgcgacg ctgccgtcga agtcgagcgc cgaggtgtcg agcaggacca cgccgtcggc
     2521 ggcgacggtg aactgcgaga ccgtggagtc gtcccggtcg cggcgaacga cctcgtcatg
     2581 ggtcgcggcg accgtggcgg cgtcggcggt gccgtgcaat tgggtgctgc gccgggcgag
     2641 ccgagcctcc tcggaggcgg tgagcaagat gcgcacgtcg gcgtcgggcg cgacaaccgt
     2701 ggtgatatcg cggccctcgg ccaccactcc cccgaagtcg tcggcgatga tcgcgatcag
     2761 gtcgcgctgc tgctcctgca ggaccgcgcg gactccgagg ttggtggcga ccttggagac
     2821 ctccgccgag acctcgctgg tgcggatcgc ttcggcgata tcgtcaccgt cgagctcgac
     2881 tgtcggatcc tgggggtcgg cgcccagccc gaggggcagg tcctcggcgg ccgcgacgac
     2941 ggcgccggcg tccgtgaggt cgatcccctg acgcaggcac cacagcgtca cggcccggta
     3001 catcgccccg gtgtcgaggt agccgaagcc gaaatgcgtt gcgacagcgc gggaaacgct
     3061 cgacttgccc gacccggagg ggccgtcgat agcgatgacg acgggagcga ccatggacgc
     3121 caccctaacc gtccgcgccg gccctcgatc gcactccgga cgaccgagaa ctgcacattg
     3181 gacgaccgag aagtgccctc gacgggggtc aatcgtggac gcgccagccg cgggcggtga
     3241 gccagtcggt gaggtcggcc gcaacagcag ggaggacgga gagttcgagc tggccgtagg
     3301 gctggccgag gttgtgttcg aggttgagct cctcgaggtt gatgcccgcg tcgccgacat
     3361 cctggaagag gcggccgagc tggcccggct cgtcggggat gaggacggtg acgacggcgt
     3421 atgccgcggg ggcggcgccg tgcttgccgg ggatgcgggc ctggccggcg ttgcccgcgg
     3481 cgatcgtccg cgcgaggacg gcgcgggcgc cgggggcctc gatctgcccg tcgggagtcg
     3541 tcgcggcggc gagtgaatca agggcgtcga cgatctggcc gaggtcggcg gccagggcct
     3601 tgaggacggc ggcgacggct ccggcgttgc cggtgaggat ctgggtccag aggaccgggt
     3661 cgctgccggc gatccgggtg acgtcgcgaa ctccggtgcc ggacagcccg accgcgtcct
     3721 cggacaggtc ggccaggcgc gccgcgacga ggctggcggc gatctgcggg acgtgcgaca
     3781 cggccgcgac ggcggcatcg tgctcggtcg gcgagagcag acggaccacg gcacccgcgg
     3841 cttcggcgat gccgcgcacc agcgccacgc cgtcgggatc cgttgtctcc gtgcgcgaaa
     3901 tcacccatgg ccggccttcg aagaggtcga tgcgggcggc gatggcaccg gacttctcgc
     3961 gcccagccat cgggtggcca ccgacatacc gttcgcggcc aggcacctcc ttggcgtcca
     4021 actcggcccg cacgatctcc ttcaccgagg ccacgtccgt gacgacagcg ttgggccatg
     4081 tcgccagcgc gtgagtgacg acatcggcgg tgacatccgg tggcgtcgcc accacgacaa
     4141 gatcgacggg gtcccccgcg ccgatccgcg ccccactgag atccgacgcc agcgcctcat
     4201 tggtcggcga ggcgtccgcg acgctgacga ccaggccggc acgcacgagc gccatcgcca
     4261 ggctggtgcc gatcaggccc gacccgatga tgtgcacccg ctgcggcagc acggggggcg
     4321 gcagagcgcg cggcatacga ctcagagccc caccgcgtca tacagctcgc tgatctcccg
     4381 ggcggtgagc tttcgcatct tgccggcctt ggtgttgccc aggcgcaccg gcccgaactg
     4441 gatgcgcgcc aactcgatca ccgggtgccc gaccgcctcc agcaggcgcc ggacgatgcg
     4501 gttgcggccc tcgtgcagga tcacttcgac gacggctttg ccgggcatgg agtcgacgag
     4561 tttgaagctg tcgacgacaa ctcgcccgtc ctccaggtcg acgccggctc gcagggtctt
     4621 gccgagatcg cggggcaccg gaccgggcac ctgggcgaca tacgtcttga gcacctcgta
     4681 tgacgggtgc gcgaggcggt tggccagctc gccgtcgttg gtgagcagca gcaacccctc
     4741 ggtgtcggcg tcgaggcggc cgacgtggaa gaggcgttcg cgccggcccg ccacgtagtc
     4801 accgacgcac gggcggccct tctcgtcctg catcgtcgtg acgactccga ccggcttgtt
     4861 gaaggcgagg tagaccttct cggggtcggt ctggacgcgc acaccgtcga cgtggatggt
     4921 ctggcgggtc gggtcgacgc gcacgccgag ttccatgacg atctggccgt ccacctcgac
     4981 ccggccctcg ctgatcatct gctcgcaagc gcgacgggag ccgattccgc tggccgccaa
     5041 cagtttttgc agccgtatgc cgtcaggatc gtgcacgtcg acgttatcgc ggtgcggttc
     5101 gacgggcacg cgtgtgggtt ggctcttctt cgtcgtgccg gcgtgctgag gcccgcggcg
     5161 gcggcccgga gtgccgccgg cctggccgcg tggccggccc ggtcgtccgc tcccaccact
     5221 acggggtttg gggggtgtca tgcgcgtccc gtctcagcga gttcgtcgag gatgtccacc
     5281 tcgggaagat acggcgccag ggccggaagt tcggacaggt cccgcagccc gagacgctcg
     5341 aggaagtagt cggtcgtgcc gtagaggatc gcccccgact ccggctcgct gccgacctcc
     5401 tcgatcagcc cgcgggtcaa cagggttcgg acgacgccgt cgacgttgac gccgcgcacc
     5461 gcgccgaccc ggccgcggga gatcggctgg cggtaggcga tgacggccaa ggtctccagc
     5521 gacgcctgcg tcagtttggc cgtcgcgccc tcgaggatga agcgctcgac cgccgcggca
     5581 tactccggcc ggctgtagat ccgccacccg cccgcgacct cgcgcaacgt gaacccccga
     5641 tcgccatagt ccgcgatcaa cccctggagg tgcgcctcga cggccggcac cgggatctcc
     5701 agcgcggtcg ccagtgccat cgacgtcacc ggatcgtcag tgaccatgag gatcgcctcg
     5761 atggccgcct tggccccgcc gggcatggcg tcgacgtcga ggacgagcgg accgtcctcc
     5821 tcggcggcac tctcgtccgc gacgaggtcc tcggaaacga ccggttcggt ctcgtgctcg
     5881 ctcactgctc gtcctctccc tcgtccgact cggcatcgtc gctctcgtcg aactcgtcgc
     5941 ttaccgtgat ctcgccggac tctgtcccga cccagcgaat tgtcaactcc cccaacgctt
     6001 ccgcttgttc gaaggtcacg ttcgcctcgc gatagagctc cagcagcgcc aggaaccggg
     6061 ccacgatgac gagtgtcgag tcggcgtccg agaccagcgc acggaatgtc gcgaccttgg
     6121 cgtggcgcag gcgctccccg atgatcccgg cctgttcctg cacgctgacg gccggggcgt
     6181 gcaagtggtc caacccgacc gtcggcaccg ccttgggtgt catcgcccgc gccgcgatca
     6241 tcgccaactg ctcgggcgtg atcccgatga ccaactccgg caacagcttt tcgaagcgcg
     6301 ggtccacccc ggcccgccgg gccaccatcg cccggttgac ggccatccgg tcctcgaaga
     6361 cgtgggcgat ctgtttgaac gcgcgatact gcagcaggcg ggcgaacagc aggtcccggg
     6421 cctcgatcag ggcgaggtcc tcctcgtcct gcggatccag ttgtggcagt aagcgagccg
     6481 ccttcaggtc caacagcgtg gcggcgacga gcaggaactc ggtcgtctgc gacaggtccc
     6541 agtcacccgc ctcacgcagc tggcgcaggt gcgcgaggaa ttcgtcggtc acctgcgcga
     6601 gcgcgatctc ggtgatgtcg agcttgtgct tggcgatcag cccgagcagc aggtcgaaag
     6661 ggccttcgaa gttgtccagg tgcaccgaga acgcgttctc cggtgcgcgc ctcgtgatga
     6721 cgccgccagg gacggcgtcc ccgctgaagt cgccctcgcc caccaggacg tccgtggtca
     6781 tcgtgctcag ggggcgctgc cgcgggcgat gagttcgcgg gcgagctggc ggtaggcgtt
     6841 ggcgccggag tgcgcggagg cgtagctggt gatcggttcg gcggcgagcg tcgcgtcggg
     6901 gaacttcacg gtgcgggaga tgaccgtatg cagcacccgg tcaccgaagt gcgcgaccac
     6961 cgactcgacg acctcgcggc tgtgcagggt gcgcccgtcg tacatcgtcg cgaggatgcc
     7021 gtcgatctgc aggcgcgggt tgagccggtc gatgatcttc tcgatcgtct cgacaagcag
     7081 cgccacgccg cgcatcgcga agtactcggt ctccaacggg atcagcaccc cgtgcgccgc
     7141 ggtgagggcg ttgaccgtca gcagccccag agagggttgg cagtcgatga ggatgacgtc
     7201 gtagtcgtcg agcaccggcc gcatgacccg tgcgaggacc tgctcgcgag cgacctcccc
     7261 cacgagttgc acctcggccg ccgacaggtc gatattggcc ggcacgacgt cgaggccggg
     7321 gacgcgtgaa tgttggatga cctcgcggat gtcgtggtcg cgttcgatga gcaggttgta
     7381 gatcgtgacg tcgaggtcgt tgctgttgat cccgagtccg accgacagcg cgccctgcgg
     7441 gtcgaagtcg atcagcagca ctttgcggcc gtattcggct agtgccgcac cgagattgat
     7501 cgtcgtcgtg gtcttgccga ccccgccctt ctggttgcac agggcgatga cgcgcgccgg
     7561 cccgtggctg gtcagcgcgg gcggctcggg gaaggccggc agttgacgcc ccgtcgggcc
     7621 gtactgaccg atctcggcgt cctcggtgcc gggcaggtgc acgtcggatc gttccacccg
     7681 gcgaccttat caagagggaa cgtggcgtcc cccatctcct cggcccgcct atcgagcgcg
     7741 ggggtgggcg ccggcataca cctcgcgcag ctggtcgatg gtcaccttgg tgtagatctg
     7801 cgtcgtcgtc accgaggcgt ggccgaggag ttcctggacg atgcgcacgt cggcgccccc
     7861 ctgcagcagg tgggtggcga aggagtggcg gagggtatgc ggtgagatct cctcggtgat
     7921 gcccgcccgc tcggcggtgg ccttgaggat cgcccacacc ccttgccggg tcagtcgccc
     7981 gccgcgctgg ttgaggaaga gggcgggtat gccgcggccc ttggctgcca ggaccggccg
     8041 ggcccgggtg cggtaggcgt cgagggcggc ggcggcatac gacccgagcg ggacgatgcg
     8101 ctccttgtcg cccttgccgc gcaagcgcac cacctcgctg tcgcgatcga ggtcgtcgac
     8161 gtcgaggtcg acgacctcgg agacccgcgc gcccacgccg tagagcacct cgagcagcgc
     8221 ccgatcccgc agtgccgcag gggtatcgcc cacactggcc gcggccagca gggcctcgac
     8281 gcggtcgagc ggaatggctt tcggcagccg cagcggcgtt ttcggcggtg tgaccgagcg
     8341 cgccgggtcg gcctccaggg cgccgtcgag ggccaggaag cggtggaagg cacgcacagc
     8401 gacgacagct cggccggccg acgacgccgc gagcggcggg tggtcgccct cgccggaccg
     8461 gagggcgacg aggaagtcgc tcacgacggc cgagtcgacc gcagcgagat cggtgatgcc
     8521 gcgagagcgc aggtaggcgg cataccgccc cacgtcccgc tcgtaggcgc gcagggtatg
     8581 ccgcgacagc ccccgctcga cgtcgaggtg ggtcagccag tcccgcaacg cccggtcgag
     8641 ggcgtcgggt aggcgttgcg gatccggcaa tgtcgttggg gcagcgtggg atcaggcgac
     8701 gatcgcggcc gtgtcggcgc actccatacc gaaggcatcg gcgacacccg ggcaggtgac
     8761 ctcgccggcg tgggtattga gtccgaaggc cagcgcgcga tcgtcgcgca tggcctgctg
     8821 ccagcccttg tcggcgagct tgacggcata cccgagcgtg gcgttggtca gcgcccacgt
     8881 ggacgtattg ggcactgcgc caggcatatt cgcgacgcag tagtagatcg agttgtgcac
     8941 ggtgaaggtg gggtcggcat gcgtggtggg ccggctgcct tcgaagcagc cgccctggtc
     9001 gatggcgacg tcgacgagca ccgatccggg cttcatggcc gcgaccatct cgtcggtgac
     9061 cagcttggga gccttggcgc ccgggacgag gacggtgccg atgaccaggt cggcctggag
     9121 cacctgctcg cgcacggcga gggcggagga ggcgatctgg tggacgcggt tgtcatagcg
     9181 ccaaaagctc attcggagct tgtcgagatc ggtgtcgagg agagtgacat cggcgcccat
     9241 gccgagcgcg atattggcgg cgttctggcc cgcgacgccg gcgccgagga cgacgacctt
     9301 ggcgttcgcg accccgccga cgccgcccat gaggacaccg cggccgccat acgggcgcat
     9361 catcgagtgt gcgccgacct gcggggcgag gcagcccgcg acctcggaca tcggatagag
     9421 cagcggcagc atgcccgacg gcagctggac ggtctcgtag gcgatgccgg tgaccttggt
     9481 ggcgacgagt tcgtcggtga gcggctggtc cgcggccagg tggaggtagg tgaagagggt
     9541 caggccctcc cggagtcggt ggtattcctc tttgaccggc tccttgacct tcatgaccat
     9601 gtcggcctcg ccccacacgt cgtcggcgga gtcgatgatg cgtgcgcctt gggagacgaa
     9661 ctcctcgtcg gtgatcgagc tgccgagtcc ggcgcccttc tggatgagca cctcgtggcc
     9721 gtgcccgacc aactcgtgca cgccgaccgg ggtgatgccc acccggtatt cgtggttctt
     9781 gacctcggtg gggacgccga tcttcacggg aactcctcgc ttcgtgcgcg actgcaactg
     9841 tgacggcagc gctggcgtca caggggcccg gtcgttgtgt gccgggcgtt cccgagtcta
     9901 tggcgttcgg gatgtcagcc gatatcggac aggccgccgc gggcctgctc cagccagctg
     9961 cgctgggcct cgagcttggc ggtcaggtcg gcgatcttcg tggagttgcc cttggcctgc
    10021 gcctgcgcga gttcctcctc gaccttggcc agcgaggcct ccagctgggt ggccagggat
    10081 tgggcccgcg cggtgacctc ggggttggtg cggcgccact tcttctcgtc ggcctcgcgc
    10141 acggccgctt cgacgcggcg catgcccttc tcggtgcgat cgatgtcggc gcgcggcacc
    10201 ttgccggcgg catcccactt gtcctggatg acgcgcaggg cggccttggt ggcctccagg
    10261 tcctgcacgg gcagcagctt ttcggcctcg gcgagcaggc cgagtttgac ctcgaggttg
    10321 gcgcgatatt cctcgtcctc ggccgcgacg acggcgtcct tggcggcgaa gaagctgtcc
    10381 tgagcggcct tgaactgctc ccacaaggcg tcgtcctcgg cgcgcgaggc gcggccggcc
    10441 tggcgccact ggtccatcag gcgcttgaaa gcgccggcgg tggccgccca gtccttgctg
    10501 gtggcgaggg cttgcgcctc cttgacgagc ttctccttgg aggagcgcgc cgcggtccgc
    10561 gtggtctcca gctccgcgaa gtgggtgcgg cgagccttgt caaaggagtt gcgggcccgg
    10621 gagaagcgct gccagagggc ggcctcgacg tccttgtcga ggcgcgcccc ggtgcgctgc
    10681 tgctccttcc aggagtcgag cagcgcccgc atcgtagcgc tcgaggtctt ccactggata
    10741 cgttccaccg gctggccggc gatggcctcc gcctgggcga ccaggtcttc gcgcaccgtc
    10801 agcgcctcgg ccttggccgc agcgcgggcc gcggactcgc tctcgcgctt gctggtgatc
    10861 tggctctcga cgccatcgac cagcgctgtc agggcaacga ggtcgccgac gacattggct
    10921 tcgccgatgt gggtgcgcag gccggtcagg ccctcggcga ggtccttgct ggtgacctcg
    10981 ggcagggcgg cgcgctgggt gagcaggtcg gccgcggcat acagctcgtc gtacttgcgg
    11041 gcgaagtact gcagggcctc ctcgtcggtc gcaccgggat aggacccgac ggcccgctcc
    11101 ccctcgccgt ccttgacgaa cacggtgccg tcctcggcga ctcggccgaa gcgggccgag
    11161 tccgacgggg tgtgcgcctg cacgggcgcg gccgccgggg gcttgggccg ggcggcgaac
    11221 atcttcgggc tgggcgccgc cgacggcttc ggcggtgccg gcaccggcgc agcagcgggc
    11281 tcgggctctg cctcgccggc gggctcggct ccaggctccg gctcgctcgc gggttcggtc
    11341 tctgggtcgg attccggctg gccggcgggt tcggcttccg ggtcggattc cggctcgctg
    11401 gcgggttcgg gttcgggctc ggattccgac tcgctggccg gctcggcttc cggctccggg
    11461 gcaagctcgt ccgggtcgtc cgggacctcg atgatctctt ccttggccgg tgcgggagcg
    11521 ggttccacga cgatggcggg ctccgcggcg aggtcgccgg gggtgtcctc ggccgcgggc
    11581 gctgcttcgg gggctggttc gggcgcctgg actacctcgt cggcactcgt ctcgtcggca
    11641 ctcgtctcga cgggggtttc gacctcggtg gtggcgtcgt tagcctcggc accggtggtg
    11701 ctcgggttgt cggtcactgc tcagcccttc gtttcggtcg tcaccgtgag cgtactgatg
    11761 gggaacttcg gggcgccatc actgccaccg tcagccaccc cgcccgcggc gatcttgtcc
    11821 acaatatcca tccccgcggt gaccgttccg aaaattgtgt agccgccgcc atcggtcggc
    11881 agctcgctgt cctggtagac gatgaagtac tgcccgccat tgctgttggg gtcctgggtg
    11941 cgcgccatgg ccaccgttcc ggccgggtac ttcccgtcgg ccggggcgtt ctcgatgccg
    12001 tagccgtagc cgggaccgcc gctaccggag ccggtcgggt ccccgcactg caggacatag
    12061 atccccgcag tggtcagccg gtggcacggc gcatccttcc agtagccctg ctgcgccaac
    12121 tggacgaacg acgcgaccgt cttcggcgcc ttggcaccgt cgagcgtgag ctcgatggca
    12181 ccgcagttgg tcgcgagcga cgcgacgacc gtcttgccct cgaccgcctt gatcgccgcc
    12241 gcgtcgggcg cgtcgagttc gccgccggtg ccgggtgcgg cgggcgccgc gtcacagccc
    12301 gcagcgacgg gggcatcgct cgcggacccg ctgccgcttg ccgacgcgct gttgcctgtc
    12361 gccccggcgg acggctgctc gttcgtgtct cccatgacct tggacagcag gaggaacgcg
    12421 gcgacgaccg cgatcacggc agctaccacc gccgcgacct gcaggtttcg cttctgcgcc
    12481 gcctgttgcg ccgcgagatt ggcctgctgg cgttcatacc ggcgcttcgc ccgcgcccgt
    12541 tcctggtcct tcgtcacgcc ggtcagtgta gatacgcggg acagcggata gtgtcagtgg
    12601 tcgtgttcat gctcgccttc cccgccgcgg ccttcgccac caactgctac gtgatcgccg
    12661 ccgcgcccgg cgaggagtgc ctcgtcgtgg acccgggcat cggggtcctg ccgaccctgc
    12721 aggagatctt ccgcgagcac cggctccgcc cagccgccgt cctgctcacc cacggccacc
    12781 tcgaccacgt ctactccgtg accccggtct gccaaggcga gacgccggcg gtggtcccgg
    12841 catacgtcca ccaggacgac cgctaccggc tcaccgaccc gatcacctat ctcgaccccg
    12901 ccatcgtcgc cgc
//
LOCUS       DAOPJG010000088        12900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5109_length_12900_cov_7.841261, whole genome shotgun sequence.
ACCESSION   DAOPJG010000088 DAOPJG010000000
VERSION     DAOPJG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12900
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5109_length_12900_cov_7.841261"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1272)
                     /locus_tag="PKU75_09255"
     CDS             complement(67..1272)
                     /locus_tag="PKU75_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPF81105.1"
                     /translation="MSIDGPLTGVGIIELGGIGPGPFAAMLLAELGADVVRIDRRDPG
                     PLAGAGSMNRSRPNIALDLKHPEAVETVRRLADSADVLIDPWRPGVAERLGLGPADLL
                     ARNPRLVFARMTGWGQDGPLAQRAGHDITYAAISGALHLSGTAEKPIAPVNVLADYAG
                     GALYLVIGILAALHDRARTGAGQVVDAAMVDGAASVVSMFYGLYGRGAWRDERGVNLL
                     DGGMPFYDTYECADGGWVAVGALEPQFWAELNRVLGVSFVADQYDPAGFAQQRQTYAA
                     AFRSRPRDEWAALFEGTDACVAPVLGLAEAPAHPHLAARGVFADVDGVALPRVAPRFS
                     AYAVPDPTPERAPGADTTAYLRAHGFSQDEVEALLVSGAAYQLPHEVLDSQARSDTSR
                     GPRTGNEGS"
     gene            complement(1351..2115)
                     /locus_tag="PKU75_09260"
     CDS             complement(1351..2115)
                     /locus_tag="PKU75_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005271601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF81106.1"
                     /translation="MELAGRSALVTGGASGLGAATVRRLYAEGAAVVIVDLPASAGAD
                     LAGELGERARFVAADVTDEGQVAAAVAAAQEVGAFSVAVNCAGIGTPGRVVGRDGAPL
                     ALETFAKVVQVNLVGTFNVIRLAAAAMVAGASPDEDRGVVVNTASIAAYDGQIGQAAY
                     AASKGGIVGMTLSIARDLAQQRIRCVTIAPGLFATPLLAGLPEEATKALGASVPHPSR
                     LGDPAEYAALVAHIVANPMLNGEVIRLDGALRMAPR"
     gene            complement(2191..2400)
                     /locus_tag="PKU75_09265"
     CDS             complement(2191..2400)
                     /locus_tag="PKU75_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPF81107.1"
                     /translation="MSGHTYGISEVVGTSDESIDAAIKSAIAKASETVRHLDWFEVGS
                     IRGHVVDGGVGHVQVTLKLGFRIED"
     gene            complement(2494..3159)
                     /locus_tag="PKU75_09270"
     CDS             complement(2494..3159)
                     /locus_tag="PKU75_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81108.1"
                     /translation="MSPSAEQDLGAIRGALVVPSLPMQLPEYAGQADAGGEIRERSVA
                     ALVEAIADWKPSAVVVVAGHDPLAEHTKGSVGARVARLLLAGAGWEGPVREISVPIDA
                     DPGELDGAAERVLEGRTATGALVLVPADLSAKRTEAAPGHFDARAAAFDAHVLDAIAS
                     GDAAALIRSDLVLAQELWATGQAPLQVLARVLPAPVRSEIMWSALPWGVQYVRARWWG
                     RKA"
     gene            complement(3156..4019)
                     /gene="folP"
                     /locus_tag="PKU75_09275"
     CDS             complement(3156..4019)
                     /gene="folP"
                     /locus_tag="PKU75_09275"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPF81109.1"
                     /translation="MTFRLGRTVFDSDARLVMAIVNRTPDSFYDKGATWDDAAAKDRV
                     RLVVDQGADIVDIGGIKAAPGVEIDAEEEKRRVVDFVAWVRGTFPDLIISVDTWRAEV
                     GRAVCEAGADLLNDAWGGADPELPAVAAEFGAALVCTHTGGVPPRTRPFRIEYDDVVD
                     DIRESLVEQAERAAALGVARDRILIDPAHDFGKNTYHSLEATRRLGELVATGWPVLVS
                     LSNKDFVGETLDKPVGERLTGTLATTAVSAWLGANVFRAHEVAETRQVLDMVSSIAGH
                     RRPARTVRGLQ"
     gene            complement(4016..5062)
                     /locus_tag="PKU75_09280"
     CDS             complement(4016..5062)
                     /locus_tag="PKU75_09280"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HPF81110.1"
                     /translation="MGECGGRGRSSRADGAAGVAAAGDLSPVGGELPVRPVHQHGEWS
                     EAQLLGAKAGQRVSVVIPARDEQDSVGDVVARVREEWVERHPLVEEVIVIDSDSQDAT
                     AERARSAGALVFAAAEVRPDLGAAQGKGEALWKALHVVTGDLLVFLDADLLDWGPHFV
                     PGLLGPLLTDPQVRLVKAVYDRPMVDDGGRQAEAGGRVTELVARPLIALHWPYLSDLV
                     QPLSGEWAIRRSTFERLSVPMGYGVEIAAVVDVAERHGADAIAQVDLGRRTHRHHRHD
                     TLGPMAVQVLAAAEARLGWRSPGRREDHVTLVQYDRTPTGFVRHERHPNIGERPPAWS
                     VEGYRNETGEDLRQ"
     gene            complement(4960..5550)
                     /locus_tag="PKU75_09285"
     CDS             complement(4960..5550)
                     /locus_tag="PKU75_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81111.1"
                     /translation="MTGGRTSDGKPRWLVGAARTVRSSGRLSALGNRAVSGVQGNRTL
                     VDVATRVFAFSGGVPLIYLKGGVAVADVPNPERLPVVLVNAVGAGVEAIPGLIEEVAG
                     LQRETLGFRPVLLVSVPCFREVRAQGWPMELVVPQQEWDFEQEWEDYLAGRVAMIATR
                     YRTWGSVGVVDGRLEPMGRRVLQQLATYRPWEASFL"
     gene            complement(5547..6662)
                     /locus_tag="PKU75_09290"
     CDS             complement(5547..6662)
                     /locus_tag="PKU75_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF81112.1"
                     /translation="MTSAGSPNPAPRRQPPGAERILLMSSNGAGMGHLTRLLAYAAHL
                     PAEVEPYVLSLSQAVPVVAQFGYRYEYLPSHGALGMTSALWQPLFQERLIETVGRVRP
                     RVVVFDGTHPYRGIDAAREVHPGVRWVWSRRGMWKPGLNGDQLAKSAWFDEVIEPGDM
                     AAEADKGASATAPAYRVGPVTLTDREDLLPRAEARAALGLPAEGRIGLISLGAGNIND
                     TSRDIGAAAAALRAAGCAVAVTDVAIAAAAHASDVHLVRAYPLARCYAAFDLVVAATG
                     YNSFHEVLRLGVPAILIPNAGTSLDDQAARATYAAQRGWALAPEAGGLDDAVRTMLSY
                     GADMAAAAQHADPGNGARAAAERLLAVAVGDVEGGRV"
     gene            6864..8126
                     /locus_tag="PKU75_09295"
     CDS             6864..8126
                     /locus_tag="PKU75_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPF81113.1"
                     /translation="MSVDVAIVGLGYVGLPLAQEASHVGLKVLGFDIGQRVVDGLNAG
                     ASHVDDLSDADIAKMLEQGFEATTDASRLAEAKTIVICVPTPLSADGGPDLGPVIAAV
                     NTIRDNLKPGMLVILESTTYPGTTDEVVRPILEETGLKAGTDFHLAFSPERIDPGNEQ
                     FGAKNTPKVVGGHTPACGDAAAAFYGQFVDTVVRAKGTREAETAKLLENTYRHINIAL
                     VNEMARFCHDLGIDLWDVIAAAKTKPFGFQAFYPGPGVGGHCIPIDPNYLSHNVRARL
                     GYPFRFVELAQEINAGMPSYVTSRAQNLLNLDSKPLRGSRVLLLGVTYKPNIADQRES
                     PAVPLAKALADKGAVVQYHDPHVPDWTAANGSASRAEDLETAVSTADLVILVQNHREY
                     DVDALAARAQRFFDTRGVAAPGEKVSRL"
     gene            8123..9025
                     /locus_tag="PKU75_09300"
     CDS             8123..9025
                     /locus_tag="PKU75_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF81114.1"
                     /translation="MTAGPEGLPQTPPALSAEEAAALAKRHGLPSLGGRPPFGDYVGS
                     LWGQRSFIWTLSTAQTVARNSSDRLGMLWTVLNPLLLIGSYYLVFGLLVPTTKGTTNF
                     IAFLAIGVVIFGYMASSITRGSKSIVNNIGLVRALRFPRAVLPVAETLTQVVTVLPAI
                     AVLMVVVIVTGEPVRWHWLLLPVALAMQTAILGGMVLIAARLVNISRDMTNLIPVAIR
                     VLRYVSGVFFSIEAYAARFDPIVGDVLMIQPFALPIALVRQCLLEEAPIQWIQWAAGL
                     GWAVVLPAFGLWFFWRDEAKYGRA"
     gene            9012..9860
                     /locus_tag="PKU75_09305"
     CDS             9012..9860
                     /locus_tag="PKU75_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81115.1"
                     /translation="MAAPDTPAGGRTSPVPERRPAAIVSHLDIIYRVVGGKRSNTSAI
                     GDDEGESIFRNLIGRGKTVATVQSVHAVKDVSFVAYHGESVGIIGRNGSGKSTLLRAV
                     AGLLPPTNGEVFLAGTPALLGVNAVLMSALSGERNIYIGGQALGLTKAQVKERFEEIV
                     AFSGIGDAVYRPMSTYSSGMAARLRFAISTAAVPDVLMIDEALSTGDAEFRKRSGEKV
                     DEIREKASTVFLVSHSNSTVRDLCDRVLWMHHGRLIADGPTEAILTAYEGWDPKKHPA
                     GPPGIG"
     gene            9871..10968
                     /locus_tag="PKU75_09310"
     CDS             9871..10968
                     /locus_tag="PKU75_09310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF81116.1"
                     /translation="MPSILVVTVVHDPEDSRIRHRQIPALLADGWQVTYAAPFRAYGR
                     PEPILGGLRTLDLPRAIGRDRMRADRAARDLIRTEAGTHDLVLVHDPELVAAVAGLRL
                     RNLVWDVHEDPAAALVAKDWLPGPAKGLVGGGWRAAERLAERRHRLLLAEYAYQDRFR
                     RPHPVVPNTVRVPAEVPAPGTDRIVYLGTVTMARGTAVMVDAARRVHAATDGGCRLDV
                     VGGARDPESARLLDDACEEGIVTWRGFVPSATALALLPGALAGLSLLQDLPNYRHSRP
                     TKIVEYMAHGVPVVTTPLPVAVDLVEQAPAGIVVPFDDPDATAAAILALREDPARAAA
                     YGAAGHAAALAAYDWNTAGPQFTATLRGFLA"
     gene            complement(10972..11223)
                     /locus_tag="PKU75_09315"
     CDS             complement(10972..11223)
                     /locus_tag="PKU75_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81117.1"
                     /translation="MFLFLDNRPGVPSEDVGDPVWTFAVDPVFPLDGDNLVVTCADGT
                     SAKVPVSDRSWLGPSAPTATRPAAPGSGDLQTAATSRPS"
     gene            complement(11223..11360)
                     /locus_tag="PKU75_09320"
     CDS             complement(11223..11360)
                     /locus_tag="PKU75_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81118.1"
                     /translation="MSRERNDFTGPGPDFFPAAERAVRRRGWTRAAASAAGVAVIAGA
                     T"
     gene            complement(11357..12067)
                     /locus_tag="PKU75_09325"
     CDS             complement(11357..12067)
                     /locus_tag="PKU75_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPF81119.1"
                     /translation="MLTACLNGARLPGEHPALPHTAEQLAADAVAVRAAGAAEVHIHL
                     KNAAGADTLTPGPLEQALRAIRAAAPGMPVGVTTGAWAQPLADARVGLIQSWRELPDH
                     ASVNWHEDGAEQVAAALLERGIGVEAGLWTPQDVDAWLRSGLRDDCLRVLLELPSRDE
                     RTTTRLADHLLARLAEAGSRIPVLLHGEEESAWPAVRHARRLGLDTRIGLEDTLTLPD
                     GSPAPDNAALFAAALNLA"
     gene            complement(12186..12773)
                     /locus_tag="PKU75_09330"
     CDS             complement(12186..12773)
                     /locus_tag="PKU75_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPF81120.1"
                     /translation="MKPGDRGAAVQRMQQRLSDLGYWLGTPDGDYGYNTTQAVMALQK
                     TAGLGRDGIYGPATRAALDRGVRPTVVVSGNAVQIDIARQVLTIVENGAIVAIVNTST
                     GSGETYISGNGQPAVATTPRGTFSVILQIDGLDKGPLGDLWRPKYFRGGYAVHGSASV
                     PAYPASHGCARVSNAVMDMIWARDLMPMGRTVVVF"
BASE COUNT         1834 a         4725 c         4526 g         1815 t
ORIGIN      
        1 gcttcagcca ggacgaggtg gaggccctgc tggtgagcgg ggcggcatac cagttgcccc
       61 acccgtctag ctgccctcgt tccccgtccg gggtccccgc gacgtatcgg agcgagcttg
      121 cgagtcgaga acttcgtggg gtaactggta tgccgccccg ctcaccagca gggcctccac
      181 ctcgtcctgg ctgaagccgt gcgcccgcag gtatgccgtg gtgtcggccc ccggggcccg
      241 ctccggggtg gggtcgggaa cggcatacgc cgagaagcgg ggggcgacgc gcggcagggc
      301 cacgccgtcg acgtcggcga agacaccgcg cgcggccagg tgcgggtgcg ccggcgcctc
      361 ggcgaggccg agcaccgggg cgacacacgc gtcggtgccc tcgaagagcg ccgcccactc
      421 gtcgcgaggg cgggagcgga aggcggcggc atacgtctgg cgctgctgcg cgaacccggc
      481 cggatcgtat tggtccgcaa cgaaactcac gcccagcacc cggttgagtt cggcccagaa
      541 ctgcggctcc agggcgccca cggcgaccca cccgccgtcg gcgcactcgt aggtgtcgta
      601 gaacggcata ccgccgtcga gcaggttgac gccgcgttcg tcgcgccagg cgccacggcc
      661 gtacagcccg tagaacatcg agacgaccga cgcggccccg tcgaccatcg ccgcgtcgac
      721 gacctggccg gccccggtgc gtgcgcgatc gtgaagtgcc gcaaggattc cgatgacgag
      781 atagagcgcg ccgccggcgt agtcggcgag cacgttgacc ggggcgatcg gtttctccgc
      841 ggtgcccgag aggtgcaggg cacccgagat cgcggcatac gtgatgtcgt gcccggcgcg
      901 ctgggcgagc ggaccgtcct ggccccaccc ggtcatccgg gcgaagacca gccgcggatt
      961 ccgggccagc aggtcagccg ggccgaggcc gaggcgctcg gcgacccccg ggcgccacgg
     1021 gtcgatgagc acgtcggcgg agtcggcgag ccggcgcacg gtctcgacgg cctccggatg
     1081 tttgaggtcg agagcgatat tgggacggga ccggttcatc gagccggccc ccgcgagcgg
     1141 accggggtcg cgccggtcga tccggacgac atcggcgccc agttcggcca gcagcatggc
     1201 cgcgaagggc ccggggccga tgccgccgag ttcgatgatg cccacccctg tcaacggccc
     1261 gtcgatgctc actcttgcga cgctaacacc gacacaaagt ccgacgtttg gcggcctaca
     1321 gcacgtcaaa cgtcggactc tgtgtcgtcc tcagcggggc gccatgcgca gggcaccgtc
     1381 gaggcggatg acctcgccgt tgagcatcgg gttggcgacg atgtgggcga ccagggcggc
     1441 atactccgcg gggtcgccga ggcggctggg gtgcgggacg ctcgcgccga gggccttggt
     1501 cgcctcctcc gggaggccgg cgagcagcgg ggtcgcgaag agcccgggcg cgatggtcac
     1561 gcaccggatg cgctgctggg ccaggtcgcg ggcgatcgag agggtcatgc cgacgatgcc
     1621 gcccttggag gctgcgtatg ccgcctgccc gatctgaccg tcgtaggccg cgatcgaggc
     1681 ggtgttgacg acgacgccgc ggtcctcgtc cgggctcgcc ccggcgacca tggcggccgc
     1741 ggcgagccgg atgacgttga aggtgccgac gaggttgacc tggacgacct tggcgaaggt
     1801 ctccagcgcc aagggcgcgc cgtcgcggcc gacgacccgg ccgggcgtgc cgatgccggc
     1861 gcagttgacg gcgacgctga aggcgccgac ctcctgggcc gccgccacgg cagcggccac
     1921 ctgcccctca tcggtcacgt cggccgcgac gaaccgggcc cgctccccca actcgcctgc
     1981 caggtcggca ccggccgacg ccggcaggtc gacgatcacc acggcggcgc cctcggcata
     2041 cagccggcgg accgtggccg cacccagccc ggacgcaccc cccgtcacga gcgcagaacg
     2101 accggccaac tccatgggga ctccttcgtc gggtgtgcgg gacctcctcg aagggtatcc
     2161 agtggcaccg gcacgatcgc ggccggctgg tcagtcctcg atgcggaagc cgagcttgag
     2221 ggtcacctgg acgtggccga cgccgccgtc gacgacatgg ccgcggatgc tgccgacctc
     2281 gaaccagtcg aggtggcgca ccgtctcgct ggccttggcg atggcgctct tgatggcggc
     2341 gtcgatggac tcgtcggagg tgccgacgac ctcggagatg ccgtaggtgt gtccggacat
     2401 ggtttcgctg cctccttcgc ggcgtcacac ggtatgacgc gtgcgggcca gtctgctccc
     2461 gtcggtcggt cccgggctag ggatggcggc gggtcaggcc ttgcggcccc accagcgggc
     2521 ccggacgtac tgaacgcccc agggcagcgc gctccacatg atctcgctgc gcaccggagc
     2581 gggtagcacc cgggcgagga cctgcagggg cgcctgcccc gtggcccaca gctcctgggc
     2641 cagaaccaga tcggaccgga tgagcgccgc ggcatcgccg gaggcgatgg cgtcgaggac
     2701 gtgcgcgtcg aatgcggcgg cgcgcgcgtc gaaatgcccg ggcgcggcct cggtgcgctt
     2761 ggcgctcagg tcggccggca cgagcacgag ggcgcccgtg gccgtgcggc cctcgagcac
     2821 ccgttccgcg gccccgtcga gttcaccggg gtcggcgtcg atcgggacgg agatctcccg
     2881 gaccggcccc tcccagccgg ccccggcgag caacaggcgc gccacgcgcg ccccgacgga
     2941 ccccttggtg tgttccgcga gcgggtcatg cccggccacg acgacgacgg ccgagggctt
     3001 ccagtccgca atcgcctcga cgagcgcagc caccgagcgc tcgcggatct caccgccggc
     3061 gtcggcctgc ccggcatact ccggcagttg catcggcagg ctgggcacga cgagagcgcc
     3121 ccggatcgcc ccgagatcct gctcagccga cggactcatt gcagacccct gaccgtgcgc
     3181 gccggccgac ggtggccggc gatgctggag accatgtcga gcacctgccg ggtctcggcc
     3241 acctcatggg cccgaaagac gttggcgccc agccaggccg agaccgctgt cgtcgcgagt
     3301 gtgccggtga gtcgctcgcc gaccggcttg tccagcgtct cgccgacgaa gtccttgttg
     3361 gacaaggaca ccagcaccgg ccagccggtg gcgaccagtt caccgagccg ccgggtcgcc
     3421 tccagcgagt ggtaggtgtt cttgccgaag tcgtgcgccg ggtcgatgag gatccggtcg
     3481 cgtgcgaccc cgagggcggc cgctcgctcg gcctgctcca ccagcgactc gcggatgtcg
     3541 tcgaccacgt cgtcgtactc gatgcgaaac ggccgggtcc gcggcggcac cccgccggtg
     3601 tgcgtgcaca cgagcgccgc cccgaattcc gctgcgacag cgggcagttc ggggtccgcg
     3661 ccgccccagg cgtcattgag caggtcggcg ccggcctcgc acaccgcgcg acccacctcg
     3721 gcacgccagg tgtcgaccga gatgatcaga tcggggaacg tgccacgcac ccacgcgacg
     3781 aagtcgacga cccggcgctt ctcctcctcg gcgtcgatct cgaccccggg cgcggccttg
     3841 atgccgccga tgtccacgat gtcggcgccc tggtcgacga ccagccggac gcggtccttg
     3901 gccgcggcgt cgtcccacgt cgcacccttg tcatagaacg agtccggggt gcgattgacg
     3961 atcgccatca ccaggcgggc atcgctgtcg aacacggtcc gccccaagcg gaaggtcatt
     4021 gccgcaaatc ctcgcccgtc tcgttccggt aaccctccac cgaccatgcc ggtggtcgct
     4081 caccaatatt gggatgacgt tcgtggcgca cgaatcccgt tggcgtgcgg tcatattgaa
     4141 ccagcgtcac atgatcctcg cggcgcccgg gtgagcgcca gccgagccgc gcctccgcgg
     4201 cggccagcac ctgcacggcc atcgggccga gcgtgtcgtg gcgatggtga cgatgcgtgc
     4261 gccgcccgag atcgacttgc gcaatcgcgt cggcgccgtg ccgctcggcc acgtccacga
     4321 ccgccgcgat ctcgacgccg tagcccatcg gcactgacag ccgctcgaag gtggagcggc
     4381 ggatcgccca ctcgccggag aggggctgga cgagatcgga gagatagggc cagtgcagcg
     4441 cgatgagggg gcgcgccacc agctccgtca cgcggccgcc cgcctcggcc tgccgtccac
     4501 cgtcgtccac catgggccgg tcgtagaccg ccttgaccag ccgcacctgc gggtcggtga
     4561 gcagcgggcc caacagcccg ggcacgaaat ggggtcccca gtcgagcagg tcggcgtcga
     4621 ggaagacgag caggtcgccg gtgacgacgt gcagggcctt ccacagtgcc tcgcccttgc
     4681 cctgtgccgc accaagatcg ggcctgacct ccgcagccgc gaacacgagg gcccccgcag
     4741 agcgcgcgcg ctccgccgtc gcgtcctggg agtcgctgtc gatgacgatc acctcctcga
     4801 ctagcggatg acgctcgacc cattcctccc ggactcgcgc gacgacatca cccaccgagt
     4861 cctgctcgtc ccgagccggg atgacgacgc tgacccgctg gccggccttg gcaccgagca
     4921 gttgggcctc gctccactcg ccgtgctggt gcaccggcct cacaggaagc tcgcctccca
     4981 cgggcgatag gtcgccagct gctgcaacac ccgccgcccc atcggctcga gacgaccgtc
     5041 cacgaccccc acactccccc atgttcgata acgcgtcgcg atcatcgcca cgcgcccggc
     5101 gagataatcc tcccactcct gctcgaagtc ccactcctgc tgcggcacaa cgagttccat
     5161 cggccagccc tgggcgcgga cctcccggaa gcacggcacg gagaccagca gcacgggccg
     5221 gaaccccagc gtctcccgtt gcagcccggc aacctcctcg atcagtcccg ggatcgcctc
     5281 cacgccggcg ccgacggcat tgaccaagac caccgggagc cgctccggat tgggcacgtc
     5341 cgccaccgcc acgccgccct tgagatagat cagaggcacc ccgccggaga aggcgaacac
     5401 ccgtgtcgcc acgtcgacca gcgtgcggtt gccctgcacc ccgctcacgg ctcggttgcc
     5461 cagcgcggac agccggcccg aggaccgcac cgtgcgcgcc gcgcccacca gccaacgcgg
     5521 cttgccatcg ctcgtgcgac cacccgtcat acccggcccc cctccacatc gcccaccgcc
     5581 acggccagga gacgctcggc ggccgcgcgg gctccgttgc cgggatcggc gtgctgcgcg
     5641 gcggcggcca tgtccgcgcc atacgacagc atcgtgcgca ccgcgtcgtc caagccaccc
     5701 gcctcggggg cgagggccca gccgcgttgg gcggcatacg tcgcgcgggc ggcctggtcg
     5761 tcgagggagg tgccggcgtt ggggatcagg atcgccggga cgccgagccg cagcacctcg
     5821 tggaaggagt tgtatcccgt cgccgcgacg acgagatcga aggcggcgta gcagcgcgcg
     5881 agcgggtagg cacgcacgag gtggacgtca ctggcgtggg ccgccgcggc gatcgcgaca
     5941 tcggtcacgg ccaccgcgca ccccgccgcc cgcaacgccg ccgccgccgc gccgatgtcg
     6001 cgcgaggtgt cgttgatatt gccggctccc aacgagatca ggccgatgcg gccctcggcc
     6061 gggagcccca gggccgcccg cgcctcggcc cgcgggagca ggtcctcccg gtcggtcagg
     6121 gtcaccgggc ccacgcggta tgccggtgcg gtcgccgagg cacccttgtc ggcttcggcc
     6181 gccatgtcac cgggctcgat cacctcgtcg aaccacgccg acttggcgag ttggtcgccg
     6241 ttcaggcccg gcttccacat gccccggcgc gaccacaccc agcgcacgcc ggggtgcacc
     6301 tcccgggccg cgtcgatgcc gcgatagggg tgggttccgt cgaagacgac gacgcggggc
     6361 cgcacccggc ccacggtctc gatgaggcgc tcctggaaga ggggctgcca caaggccgag
     6421 gtcatcccga gggcgccgtg cgagggcagg tattcatagc ggtagccgaa ctgcgcgacc
     6481 acgggcacgg cctgcgagag cgagagcacg tagggctcga cctcggccgg caggtgcgcg
     6541 gcatacgcga gcagccgggt caggtgaccc atgccggctc cattgctcga catgagcagg
     6601 atgcgctcgg cgccgggcgg ttgccggcgc ggtgcagggt tcggcgatcc ggcgctggtc
     6661 atggtggccc gatcatgtca cgctcgcccc ggcgggccgg cgtgcctggc acccccgggc
     6721 gcactgtgtc gtggatcggg ggcccgcagg ggtgcggata gaatgttgtg gatctgagga
     6781 ctcccgctgc acggagtcgc gcaggtcgcg atcgccaccg cttgccatcc cgcgggctct
     6841 cggcccatca aggagaaaca cgtatgtccg ttgacgttgc catcgtcggc ctcgggtatg
     6901 tcggcctgcc gctcgcgcag gaggcctccc acgtgggctt gaaggtgctg ggcttcgaca
     6961 tcggccagcg ggtcgtggac ggcctcaacg ccggcgcctc ccacgtcgac gacctcagtg
     7021 acgccgacat cgccaagatg ctcgagcagg gcttcgaggc gacgaccgac gcgagccggc
     7081 tggcggaagc caagacgatc gtgatctgcg tgccgacgcc gctctcggcg gacgggggcc
     7141 cggacctcgg gcccgtcatc gcggccgtca acaccatccg cgacaacctc aagcccggga
     7201 tgctcgtcat cctcgagtcg acgacctacc ccggcacgac cgacgaggtc gtccgcccga
     7261 tcctcgagga gaccggcctg aaggccggca ccgacttcca cctcgccttc tcccccgagc
     7321 gcatcgaccc gggcaacgag cagttcggcg ccaagaacac ccccaaggtc gtcggcggcc
     7381 acacccccgc gtgtggcgac gcggccgcgg ccttctacgg ccagttcgtc gacaccgtcg
     7441 tgcgggccaa gggcacccgc gaggccgaga cggccaagct gctcgagaac acctaccggc
     7501 acatcaatat cgccctcgtc aacgagatgg cccgcttctg ccatgacctc ggcatcgacc
     7561 tgtgggatgt catcgcggcc gcgaagacca agccgttcgg cttccaggcg ttctatcccg
     7621 gccccggcgt cggcggccac tgcatcccga tcgaccccaa ctacctctcg cacaatgtcc
     7681 gcgcccgcct cggctacccg ttccggttcg tcgagctggc ccaggagatc aacgcgggta
     7741 tgccgtccta tgtcacctcg cgcgcgcaga acctgctcaa cctcgacagc aagccgctcc
     7801 ggggctcgag ggtgctgctg ctcggcgtga cctacaagcc caatatcgcc gaccagcgtg
     7861 agagcccggc cgtgccgctg gcgaaggcgc tggccgacaa gggcgcagtc gtgcagtatc
     7921 acgacccgca cgtgcccgac tggacggccg ccaatgggtc cgcgagccgc gccgaggacc
     7981 tggagaccgc cgtgtccacc gccgacctgg tcatcctcgt gcaaaaccac cgggagtatg
     8041 acgtggacgc cctcgccgcg cgcgcgcagc ggttcttcga cacccggggc gtcgccgccc
     8101 cgggcgagaa ggtctcccgc ctgtgaccgc aggcccggag ggtctgccgc agacccctcc
     8161 ggcgctgagc gcggaggagg ctgcggcgct ggccaagcgc cacggcctgc cgtcgctcgg
     8221 cggacggccg ccgttcggcg attacgtggg cagcctgtgg gggcagcgct cgttcatctg
     8281 gaccctgtcg accgcgcaga ccgtggcgcg caacagctcc gaccggctcg gcatgctgtg
     8341 gacagtcctg aacccgctgc tgctcatcgg cagctactac ctcgtcttcg gcctgctcgt
     8401 cccgacgacg aagggcacga ccaacttcat cgccttcctc gccatcggcg tcgtgatctt
     8461 cggctacatg gcctcctcca tcacccgggg ctcgaagtcg atcgtcaaca acatcggtct
     8521 cgtgcgcgcg cttcgcttcc cgcgggcggt gctgccggtc gccgagacgc tgacccaggt
     8581 cgtgacagtc ctgccggcta tcgcggtcct gatggtcgtc gtcatcgtca cgggtgagcc
     8641 tgtccgctgg cactggctgc tgctcccggt ggcgctggcg atgcagacgg cgatcctcgg
     8701 cggcatggtc ctcatcgcgg cgcggctggt caatatctcg cgggacatga ccaacctgat
     8761 cccggtggcg atccgggttc tgcgctatgt ctccggcgtg ttcttcagca tcgaggcgta
     8821 tgccgcgcgc ttcgacccca tcgtcggcga cgtgctgatg atccagccct tcgcgctgcc
     8881 gatcgcgctg gtgcgccagt gcctcctcga ggaggcgccg atccagtgga tccagtgggc
     8941 tgcggggctc gggtgggccg tcgtgttgcc ggccttcggg ctgtggttct tctggcggga
     9001 cgaggcgaag tatggccgcg cctgacaccc ccgccggcgg ccgcacgtca ccggtccccg
     9061 agcgccggcc cgccgcgatc gtctcccacc tcgacatcat ttatcgggtc gtcggcggca
     9121 agcgttccaa cacctccgcc atcggcgacg acgagggcga gtcgatcttc cgcaacctga
     9181 tcggccgggg caagacggtc gcgaccgtgc agagcgtgca tgccgtcaag gacgtctcgt
     9241 tcgtcgccta tcacggtgag tccgtcggga tcatcggccg caacggctcg ggcaagtcga
     9301 ccctgctgcg cgccgtcgcc ggcctgctgc cgcccaccaa cggcgaggtc ttcctcgccg
     9361 gcaccccggc cctgctcggc gtcaacgcgg tcctgatgag cgcgctgagc ggcgaacgca
     9421 atatctacat cggcggtcag gccctgggcc tgaccaaggc ccaggtcaag gagcggttcg
     9481 aggagatcgt cgccttctcc ggcatcgggg acgccgtcta ccggccgatg tcgacctact
     9541 cctcgggcat ggcagcccgg ctgcgattcg ccatctcgac cgccgccgtc cccgatgtgc
     9601 tgatgatcga cgaggcgctc tcgaccggcg atgcggagtt ccgcaagcgc agcggggaga
     9661 aggtcgacga gatccgcgag aaggcctcga cggtcttcct cgtctcgcac tccaactcca
     9721 ccgtccgcga cctgtgcgac cgcgtcctgt ggatgcacca cggccgcctc atcgccgacg
     9781 gccccaccga ggccatcctg acggcctacg agggctggga ccccaagaaa caccccgccg
     9841 gcccgcccgg catcggctga gcagcgccgt atgccgagca tcctcgtcgt cacggtcgtg
     9901 cacgaccccg aagactcccg catccgccac cggcagatcc ccgcactgct cgccgacggt
     9961 tggcaggtga catatgccgc gcccttccgg gcatacggcc gtcccgagcc catcctcggc
    10021 gggctgcgga ccctcgacct gccccgcgcg atcggccggg accggatgcg cgccgaccgg
    10081 gccgcccgcg acctgatccg aaccgaggcc ggcacccacg acctggtcct ggtccatgac
    10141 cccgaactcg tcgccgccgt cgccgggctg cggctgcgca atctcgtctg ggacgtccac
    10201 gaggaccccg ctgccgcgct cgtcgccaag gactggctgc ccggtccggc caaaggtctg
    10261 gtcggcggcg gctggcgcgc ggccgaacgc ctggccgagc gccgccaccg gctcctgctc
    10321 gccgaatacg cctaccagga ccgtttccgg cgtccccatc ccgtcgtgcc caacacggtg
    10381 cgcgtgcccg ccgaggtgcc ggcgccgggg accgaccgga tcgtctatct cggcacggtg
    10441 acgatggccc gcggcaccgc cgtcatggtc gacgccgcgc gccgggtcca cgcggccacc
    10501 gacggaggct gccgcctcga cgtcgtcggc ggggcgcgcg accccgagtc cgcccgcctg
    10561 ctcgacgacg catgcgagga ggggatcgtg acatggcgtg gcttcgtgcc cagcgccacc
    10621 gcgctggcct tgctccccgg cgccctggcc ggcctgtcac tcctgcagga cctgccgaac
    10681 taccgccact cgcggccgac caagatcgtc gagtacatgg cgcacggcgt ccccgtggtg
    10741 acgacgccgc tgccggtcgc cgtcgacctc gtggagcagg cgccggcggg catcgtcgtg
    10801 cccttcgacg accccgacgc gaccgccgca gcaatcctgg cgctgcggga ggatccggcg
    10861 cgggcagcgg cctacggtgc ggcgggacac gcggcggcgc tcgcggcata cgactggaac
    10921 accgccggcc cccagttcac cgcgactttg cgcgggttcc tggcctaagc ctcaactcgg
    10981 gcgcgatgtc gcggcggtct gcaagtcgcc cgagccgggg gcggcgggcc gggtggcggt
    11041 gggggccgac ggcccgagcc acgacctatc ggacacgggc accttcgcgc tcgtgccgtc
    11101 ggcgcaggtc acgaccaggt tgtcgccgtc gagcgggaag accgggtcga ccgcgaaagt
    11161 ccacaccggg tcgccgacgt cctcggaggg caccccgggc cggttgtcga ggaagaggaa
    11221 catcaggtgg cgcccgcgat cacggccaca cccgccgcgg acgcggcggc ccgggtccag
    11281 ccgcggcgcc ggacggcccg ctcggctgcc gggaagaagt ccggcccggg gccggtgaag
    11341 tcgtttcgtt cccgactcac gcgagattca gcgcggcggc aaagagagcg gcgttgtccg
    11401 gcgcgggcga gccgtcgggc aaggtgaggg tgtcctcgag gccgatgcga gtgtcgaggc
    11461 cgaggcggcg cgcgtgccgc acggccggcc aggcgctctc ctcctccccg tgcaggagca
    11521 ccggtatgcg gctgcccgcc tccgcgagtc gtgccagcag gtggtcggcg agccgtgtcg
    11581 tcgtccgctc gtcacggctg ggtagctcga gcagcacccg caggcagtcg tcgcgcaggc
    11641 ccgagcgtag ccacgcgtcg acgtcctggg gagtccacag gcccgcctcg acgccgatgc
    11701 cccgctcgag cagggccgcc gccacctgct cggcgccgtc ctcgtgccag ttgacggagg
    11761 cgtggtcggg cagttcgcgc cacgactgga tcagcccgac ccgggcgtcg gcgaggggct
    11821 gggcccaggc gccggtggtg accccgaccg gcatacccgg cgcggccgcg cggatcgctc
    11881 gcagggcctg ctcgagcggg ccgggggtca gggtgtcggc gccggcggcg ttcttgagat
    11941 ggatgtgcac ctcggccgca ccggcggccc gcacggccac ggcgtccgcc gccaactgct
    12001 cggccgtatg cggaagcgcc gggtgctccc ccgggagccg ggcgccgttg aggcaggcgg
    12061 tgagcatcct ccgaacctag cgaccgcggg ccgtgacgac aatgcgcccc cgccctttcc
    12121 cgtcgaaagc gcacgacacg ccgtatggcg cgaccattac ggcgtgtcgt gcactctcga
    12181 ccgggtcaga acacgaccac ggtccggccc atcggcatca ggtcgcgggc ccagatcatg
    12241 tccatgacgg cattgctcac ccgggcgcag ccgtgtgagg cgggataggc ggggaccgag
    12301 gcggagccgt gcacggcata gccgccgcgg aagtacttgg ggcgccacaa gtccccgagg
    12361 gggcccttgt cgaggccgtc gatctgcagg atgaccgaga aggtgccgcg gggggtggtg
    12421 gcgacggcgg gctggccgtt gccgctgatg taggtctcgc cgctgccggt gctggtgttg
    12481 acgatcgcga cgatcgcgcc gttctcgacg atggtcagga cctggcgggc gatgtcgatc
    12541 tgcacggcgt tgccgctgac gacgaccgtg ggcctgacgc cccggtcgag cgcggcccgg
    12601 gtggccgggc cgtagatgcc gtcgcggccg aggccggccg tcttctgcag ggccatgacg
    12661 gcttgcgtgg tgttgtagcc gtagtctccg tcgggggtgc cgagccagta gccgaggtcg
    12721 gacaggcgct gctgcatccg ctgcaccgcg gcgccccggt cgcccggctt catggtcgag
    12781 gtgtcggtca ggggcggcgg ggtggtggcg gtcggcttcg gcgtggtcgg gctcggcgtg
    12841 gtcgggctcg aggtgctggt cgtgcttgac ggcgtggatg tgcctgacgt gctggtcgtg
//
LOCUS       DAOPJG010000089        12823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5153_length_12823_cov_7.332863, whole genome shotgun sequence.
ACCESSION   DAOPJG010000089 DAOPJG010000000
VERSION     DAOPJG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5153_length_12823_cov_7.332863"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKU75_09335"
     CDS             complement(<1..702)
                     /locus_tag="PKU75_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPF81121.1"
                     /translation="MTTTSSPGLATRAVHAGRDDFTDRGVHALPIDLSTTYPLADVEA
                     GGAAYDHLSGGRPPETGDSYVYARLWNPTVAHFEAGIAALEGGSDAIAFASGMAATTA
                     LLMSRVAAGKPHVVAIRPLYGGTDHLLATGLLGTEVTYAREGEVASAITERTGLVFLE
                     SPGNPTLDLIDIAAVTRAAGGVPVVVDNTFATPVLQRPLEHGARVVLHSATKFLGGHG
                     DAMGGVLVTDSETAAT"
     gene            812..1291
                     /locus_tag="PKU75_09340"
     CDS             812..1291
                     /locus_tag="PKU75_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPF81122.1"
                     /translation="MAKEIRPSVDLDDVDLALVDALVADARIANNRLAARAGVAASTA
                     LARVRQLIDRGIVRGFHADLDPERIGRPVQAVVAIRLRAHDRRHIDAFTATVPRLPEV
                     VQSFHLAGDDDYLLHVAVPSASYLRDWILDEVTTHPAVAHSRTSLVFGHERGQPGPL"
     gene            complement(1497..3167)
                     /gene="pgm"
                     /locus_tag="PKU75_09345"
     CDS             complement(1497..3167)
                     /gene="pgm"
                     /locus_tag="PKU75_09345"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HPF81123.1"
                     /translation="MTIHERAGQPAQPSDLVDVARLVTAYYTGKPDPENIDQQVAFGT
                     SGHRGSSLKTAFNEDHILATTQAICDYRREQGFDGPLFIGRDTHGLSEPAWASALEVL
                     IANDVTVLVDADDRYTPTPAVSHAILTANRGKIGGVDDLPAGTGLADGIVVTPSHNPP
                     TDGGFKYNPPHGGPADTDATKAIAAAANAYIKAGLEGVRRVPFTRARAACSAYDFLGT
                     YVADLPNVVDIAAIKEAGIRIGADPLGGAAVDYWGAIAERHRLDMTVVNPLVDPTWRF
                     MTLDWDGKIRMDCSSPSAMASLIDKRDQFQIATGNDADADRHGIVTPDAGLMNPNHYL
                     SVAIQYLYGGARPGWRSDGFIGKTLVSSSMIDRVADDLGRRLVEVPVGFKWFVPGLLD
                     GSGPFGGEESAGASFLRFDGTVWTTDKDGIILALLASEIQAKTGKSPSQLYAELTAKH
                     GSPAYARIDAPADREQKAKLAALSPADVTAETLAGEAITAKLTEAPGNGAKIGGLKVV
                     TESAWFAARPSGTEDVYKIYAESFQGPEHLAQVQAQAHDVVSAALGVS"
     gene            complement(3213..5231)
                     /locus_tag="PKU75_09350"
     CDS             complement(3213..5231)
                     /locus_tag="PKU75_09350"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HPF81124.1"
                     /translation="MKVSPGASRPISPLRRAAVAVAAAGSALALVAGCTGTSGPSGTA
                     GPVGRAGGGAFTADPAAALQPFASCDQLLDYFRSEAKPLVGPYGLAGGPVAYNAGMAV
                     EGATPSAGATGAAAGSGSSADQARSGTNNQEDGVDEADTVKTDGTIIVTSLNGRIQVT
                     DADAGRLLSTITIPRAGSVSAELLLSGRTLIVLSTSTDAYPTLSDGPERLTVPAFAPQ
                     RTTVTRVDLTDPSAPKVLGASRIEGGYRSARMIGTSVRLVLVSEPTGLRFTQPKSGGL
                     DAEEDATRANKDVIAKSTIDDWVPHVQTLDAEGNASKAQRLIDCAAIARPDEFAGLST
                     LSVLTFDAGSADGRVTPTSTVGVVATGETVYASTDRLIVATSPWDIWNLGWLRRSDMS
                     KLKTGLHSFDISDPATTRYAGSGKVAGRLVNQFALDEDRGVIRVATTTSDLQWSTDTQ
                     SSLLVLKEQGDKLVTVGRVDGMGLTEHIRSVRYLSPTLAAVVTFRQTDPLYLVDTSDP
                     TKPKVAGELKIPGYSAYLHPVGDGLLLGVGQDADAETGAVRGLQVSLFDVGDAARPTR
                     LQQLTWKNANSPVEWDHRAFLWWKDTAYVPATLWSNSRDFTGVLSARLAGRSLADGPR
                     AASSRSGYDDSPSRVFVIGDNLWTLGYRGLQRFDLATLTPEKTIPLPS"
     gene            complement(5307..7544)
                     /gene="glgB"
                     /locus_tag="PKU75_09355"
     CDS             complement(5307..7544)
                     /gene="glgB"
                     /locus_tag="PKU75_09355"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPF81125.1"
                     /translation="MPTPPSALPGARWSLANGRHQQPHDLLGQHIEAEGLRIRVMRPL
                     AQRVRVRFEDGEISELSHESDGIWSGLRPGATNALDYRILTTWEDGVEHEQDDPYRFA
                     PTLGDIDRFLINEGRHEKLWQVLGAHVHTYDGPMGSVTGTSFAVWAPRAKAVALVGDF
                     NGWDNRSHPMRLLGNSGVWELFVPGVGPGSTYKFRIRGADDVERDKADPMALRTEVPP
                     RTGSVVHDSAYQWADSEWETRKRETNPHTGPMSIYEVHLGSWRAGLTYVELAEHLVNY
                     VQDLGFTHVEFLPVMEHPYGPSWGYQVTGYYAPTARFGTPDDFKYLVDKLHQAGIGVI
                     LDWVPAHFPRDEWALARFDGLALYEHADPRRGDQPDWGTHIFDFGRKEVRNFLVANAL
                     YWMEEFHADGVRVDAVASMLYLDYSRRSGEWLPNQYGGRENIEAIGLLQETNATAYKL
                     HPGIVTVAEESTSWPGVTKETASGGLGFGLKWNMGWMNDSLRYLQRDPVHRQYHHNLL
                     TFSLMYAYSENYILPISHDEVVHGKGSLLGKIPGHRYDQLATLRAYLAYIWSHPGKQL
                     LFMGCEFAQPSEWADGHGLDWWLLDHAAHYRVHNLVKELNQIYKAHDALWAQDSSPAG
                     FEWLNPHDNAGNTLSYVRFGNPDRGGDSAVVALINFGGNARDGMRFGLPYAGEWEVIM
                     DTSGFDEYGSPSQSGVIVTAEPTPWDNQPFSASLSVPKLAALYLAPHRPEPEADEAAE
                     AAPTA"
     gene            complement(7547..8986)
                     /locus_tag="PKU75_09360"
     CDS             complement(7547..8986)
                     /locus_tag="PKU75_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HPF81126.1"
                     /translation="MAILHFGASITPTKLELLTGWIGSQRWYAGKGSTPQPRRLFSWR
                     LDDPEGEVGIESIILADDSSSPPVVYQVPLTYRSAPLAGAAAALVGVVEHSVLGTRYV
                     YDAPHDPVFAERLFALATGQAQAASSSANDTVEETVTGTGTPNGLRLTSSRVLTGEQS
                     NTSIISIAVDEAGAEHPVITKVFRALADGDNPDVILQGALASAGCTCVPASLGAVAGS
                     WPGADGDSTAHGHLAFVQEFLPGAQDAWREALVAAGRGEDFTEQARTLGAATAQVHAI
                     LRDTLPAEAAEAADEGRIAETIEEMRTRFRSALAEVPDLQAHAVGIDGLYAAAAAASW
                     PDFQRIHGDYHLGQVLDVPERGWVLLDFEGEPLRPLAERNRTDQPLRDIAGMLRSFDY
                     AGGSVEQQNRGESARDWVTATQEAFLAGYTEESGHDPRDAGPLLSAYIVDKALYEVVY
                     EARNRPDWLTIPLAAIDRLLPTALPTKEN"
     gene            complement(8991..11000)
                     /locus_tag="PKU75_09365"
     CDS             complement(8991..11000)
                     /locus_tag="PKU75_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HPF81127.1"
                     /translation="MTTPPQTPIGRIPVQDVKPLVDGGARPAKAVVGETFTISATVFR
                     EGHDAVNATVVLTRPDGSTQHLPMDCINPGLNLWIAEVTPDAVGDWTYRVEGWGDPFG
                     TWEHDATIKVAAGVDVELMLTEGALLLERAAAQPGRSGEGRLLLDEAVSRLRDSRLEP
                     AVRLHGGVHPDVHAEMDRLPMREYVSPTADFPLLVERERALYGAWYEIFPRSEGCYYD
                     ESAGGWISGTLRTATERLPAIAAMGFDVVYLTPIHPIGTTARKGPNNTLDAGPRDPGS
                     PYAIGSPDGGHDAIHPDLGTFDDFDFFVAEAGRHGLEVAMDIALQASPDHPWVQSHPE
                     LFTHRADGTIAYAENPPKKYQDIYPLNFDNDPEKAYAEVRRVMQVWIDHGVKIFRVDN
                     PHTKPVEFWQWLIADVAKDHPDVIWLAEAFTKPAMMHTLGKVGFQQSYTYYAWRNQPW
                     DLREYVEELAGEAAAYMRPSFWPTTHDILTPYMQFGGPAAWKVRAALAATLVPTYGIY
                     TGYELMEHVARPGAEEQIDNEKYQFKDRRWMDYEEGGPRSGQTLAPYLTRLNEIRRAH
                     PALHWLRNITFHTVADENLLCFSKHRVLPSGEVDTIIVVANLDPHSTRSSIVSLNMPA
                     LGMDWGDSFTAHDEISGADWQWGERNYVRLGPEWEPVHIIHVRRG"
     gene            complement(11070..>12823)
                     /gene="glgX"
                     /locus_tag="PKU75_09370"
     CDS             complement(11070..>12823)
                     /gene="glgX"
                     /locus_tag="PKU75_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HPF81128.1"
                     /translation="LDPYARALDFGGSAVVIDPTFDWGGDVPPRHPWSSSVLYEGHVR
                     GLTMLHPGIPSHLRGTYAGLGHPALIEHLHRIGVTTLELLPVHAFVSEPALLQRGLIN
                     YWGYNSLGFFAPHGRYAAAADPQGVVDEFKAMVRALHAGGIEVILDVVYNHTAEQGID
                     GPTLSWRGLDAAAYYRIDAFGRDVDMSGCGNTFDLCHPVACQLVLDSLRYWVGEMHVD
                     GFRFDLAVALGRDKNGDYDPDHPFLVALRTDPLLSTTKLVAEPWDIGFGGWRTGQFPP
                     PFSEWNDRFRDDVRTFWLSDVASVLGGGPGHGSRMLATRLAGSQDLFGHGHRAPTASI
                     NFVTAHDGFTAADLTAYDEKHNEANGEGNRDGASHNRSWNHTVEGPTDDEAVNAARRR
                     SIRNLLGTLLISSGVPMLCAGDEFGRTQYGNNNAYCQDNEISWLDWRWEPWQAELVDQ
                     VAALTRLRREHAVLRQRRFLTGDPVYEGGPADVSWRSPEGTPLGAQEWEDPATRSFSM
                     LLGSANAAETDILLIVNGQTHPVQMTLPDIPGRQRYRLMWDSAAEEADLSREDERGFG
                     DQVKAAPCSLQVWASVG"
BASE COUNT         1816 a         4290 c         4678 g         2039 t
ORIGIN      
        1 ggtggcggcg gtctcgctgt ccgtgacgag cacgccaccc atcgcatcgc cgtgtccgcc
       61 aaggaatttc gtcgccgaat gcaggacgac gcgagcgccg tgctcgagcg ggcgctgcaa
      121 caccggcgtg gcgaaggtgt tgtccacgac caccggaaca ccgcccgcgg cacgggtgac
      181 ggcggctatg tcgatcaggt cgagagtggg gttgccgggg gactcgagga agaccagacc
      241 cgtgcgctcg gtgatggccg acgcgacctc gccttcccgg gcataggtga cctcggtgcc
      301 gagcagcccc gtcgccagca ggtgatcggt gccgccatac aggggccgga tggccacgac
      361 gtgcggcttg cccgccgcga cgcgcgacat cagcagcgcg gtcgtcgccg ccatgccgct
      421 ggcgaaggcg atggcgtcgc tcccgccctc cagcgcggcg atgcccgcct cgaagtgggc
      481 gaccgtcgga ttccacagcc gggcgtagac ataggaatcg cctgtctccg ggggccggcc
      541 gccgctcaga tggtcgtatg ccgcgccccc cgcctcgaca tccgcgagcg ggtaggtcgt
      601 cgacaggtcg atgggcaggg cgtgcacgcc gcggtcggtg aagtcatcgc ggccggcatg
      661 gacggcacgg gttgcaagac cggggctgga cgtcgtcgtc atcttccgag ggtcccaggg
      721 ttgcagggtg agcgggtact ccgccgaaca agtgcccgag ccggcaccgt tggcggcccc
      781 gctggcgaac aatcatcgct attctgcctc gatggcgaag gaaattcggc ccagtgtgga
      841 tcttgacgac gtcgacctcg ccctcgtcga cgccctcgtt gccgacgccc gcatcgcgaa
      901 caaccgcctc gcggcgcgag ccggcgtggc cgcctcgacc gccctggccc gggttcggca
      961 gctgatcgac cgggggatcg tgcggggttt ccacgccgac ctcgaccccg aacggatcgg
     1021 gcgcccggtc caggcggtcg tcgccatccg gctgcgcgcc cacgatcgcc ggcacatcga
     1081 cgccttcacc gccaccgtgc cccgcctccc cgaggtggtg cagagcttcc acctcgccgg
     1141 cgacgacgac tacctgctcc acgtggctgt gccctccgcg agctacctgc gcgactggat
     1201 cctcgatgag gtcaccaccc acccggcggt cgcgcacagc cgcaccagcc tcgtcttcgg
     1261 ccacgagcgc ggtcagcccg ggccgctgtg accggtgccc cgcgtcaggg gcaatccgcg
     1321 gccgtggagg ctgcggcggg ccgccgccta caagaactcg atcggctccc cgccgcgccc
     1381 gcgtgacagg ggccggctca cggacttcct gtcgtcagcg gcccggttgt ccactggaca
     1441 gcggcccggt ttcgactggc cagcggcccg gttgtccacg tgcccggccg ccggcgtcag
     1501 gacacgccga gggccgcgct gacgacgtcg tgggcctgcg cctgcacctg cgcgaggtgt
     1561 tcggggcctt ggaaggattc ggcgtagatc ttgtagacgt cctcggtgcc gctggggcgg
     1621 gcagcgaacc acgccgactc ggtgacgacc ttcaggccgc cgatcttggc cccgttgccg
     1681 ggagcctcgg tcagcttcgc cgtgatcgct tctcccgcaa gggtttcggc agtgacgtcg
     1741 gcgggggaga gcgcggccag cttggccttc tgctcccggt cggccggggc gtcgatgcgg
     1801 gcgtaggccg gactgccgtg cttggcggtc aactcggcat acagctggct cgggctcttg
     1861 cccgtcttgg cctggatctc cgaggccagc agcgcgagga tgatgccgtc cttgtccgtc
     1921 gtccacaccg tgccgtcgaa gcgcaggaag gacgccccgg ccgactcctc gccaccgaag
     1981 gggccggaac cgtcgagcag gcccggcacg aaccacttga acccgaccgg gacctccacg
     2041 agccggcggc ccagatcgtc cgcgacccga tcgatcatcg aggacgacac cagcgtcttg
     2101 ccgatgaacc cgtcggagcg ccagcccggc cgggcaccgc catacaagta ctggatcgcc
     2161 acggagagat agtggttcgg gttcatcagc ccggcgtcgg gcgtgacgat gccgtgccgg
     2221 tcggcgtcgg cgtcattgcc cgtcgcgatc tggaactggt cgcgcttgtc gatgagggaa
     2281 gccatcgccg acggcgacga gcagtccatg cggatcttgc cgtcccagtc gagcgtcatg
     2341 aaccgccacg tcgggtccac gagcggattg acgactgtca tgtcgagcct gtgccgctcg
     2401 gcgatcgcgc cccaatagtc caccgcggcc ccaccgagcg ggtcggcccc gatgcggatg
     2461 ccggcctcct tgatcgccgc gatgtcgacg acattgggca ggtcggcgac ataggtgccg
     2521 aggaagtcgt atgccgagca ggccgcccgc gctcgggtga agggcacccg ccggacgccc
     2581 tccagcccgg ccttgatgta ggcattcgca gctgccgcaa tggctttcgt cgcatcggtg
     2641 tcggccgggc cgccgtgcgg cgggttgtac ttgaaaccgc cgtcggtcgg cggattgtgc
     2701 gacggggtga cgacgatgcc gtcggccagg ccggtgcccg ccggcaggtc gtcgactccg
     2761 ccgatcttgc cgcggttggc ggtcaggatg gcatgcgaca ccgccggcgt cggggtgtag
     2821 cggtcgtcgg cgtcgacaag cacggtcacg tcgttggcga tcagcacctc gagcgccgac
     2881 gcccacgccg gctcggacaa cccgtgcgtg tcgcggccga tgaagagcgg gccgtcgaag
     2941 ccctgctcgc ggcggtagtc gcagatggcc tgcgtggtcg cgaggatgtg gtcctcgttg
     3001 aacgcggtct tcagcgacga cccccggtgc ccggaggtgc cgaaggcgac ctgctggtcg
     3061 atattctccg ggtccggctt gccggtgtag taggccgtca ccagacgggc gacatcgacc
     3121 agatcggagg gctgggccgg ctgtcctgca cgctcgtgga tcgtcatgcc cccatccaag
     3181 cactgccccc gccgggtcgc cacgggcgcc cgtcagctgg ggagcgggat ggtcttctcc
     3241 ggcgtcaagg tggcgaggtc gaaacgttgc agaccgcggt agccgagggt ccacaggttg
     3301 tccccgatga cgaacacccg gctcgggctg tcgtcgtagc cggaccggct gctcgccgcg
     3361 cgcgggccgt cggcgagcga acggccggcg agccgggcgc tcagcacgcc ggtgaagtcg
     3421 cggctgttac tccacagcgt cgccgggacg tatgcggtgt ccttccacca caagaatgcg
     3481 cggtgatccc actcgacggg tgagttggcg ttcttccacg tcagctgctg cagtcgcgtc
     3541 ggtcgcgccg cgtcgccgac gtcgaacagc gagacctgca atcccctgac ggccccggtc
     3601 tcggcgtccg cgtcctgccc gacgccgagc agcaggccgt cgccgacggg atggaggtat
     3661 gccgagtagc cgggaatctt caactcgccc gccaccttcg gcttggtcgg gtcgctggtg
     3721 tcgacgagat agagcggatc ggtctggcgg aaggtgacga ccgcagcgag ggtgggggag
     3781 aggtagcgca ccgagcggat gtgctcggtc agacccatcc cgtcgacccg gccgacggtg
     3841 acgagcttgt cgccctgctc cttcagcacc agcagggacg attgggtgtc cgtcgaccac
     3901 tggaggtcgg aggtcgtcgt cgccacccgg atcacgcccc tgtcctcgtc gagggcgaac
     3961 tggttgacca gcctcccggc gaccttgccg gaccccgcat agcgcgtcgt ggccgggtcg
     4021 ctgatgtcga aggagtgcag cccggtcttg agcttgctca tatcggagcg gcgcagccat
     4081 cccaggttcc agatgtccca cgggctggtg gcgacgatga gccggtcggt ggaggcatag
     4141 accgtctcgc cggtcgcgac cacacccacc gtggacgtcg gcgtgacccg cccgtcggcg
     4201 gacccggcgt cgaaggtcag gaccgacagc gtggacaacc cggcgaactc gtccggccgg
     4261 gcgatcgcgg cacagtcgat cagccgctgg gccttcgagg cgttgccctc ggcgtcgagg
     4321 gtctgcacgt gcggcaccca gtcgtcgatc gtgctcttgg cgatcacgtc cttgttggcc
     4381 cgcgtcgcgt cctcttccgc atcgaggccg ccgctcttcg gctgggtgaa cctcagcccg
     4441 gtgggctcgg agaccaggac cagccgcacg gaggtgccga tcatccgggc cgaacggtag
     4501 ccaccctcga tccgtgacgc cccgagcacc ttcggggcgc tcgggtcggt gagatcgacg
     4561 cgggtgaccg tggtccgttg cggggcgaag gcgggcacgg tcagcctttc cggcccgtcc
     4621 gacaaggtgg ggtaggcatc ggtgctggtg ctcaggacga tcagggtgcg acccgacagc
     4681 agcagttcgg ccgagacgct tccggcccgg gggatggtga tcgtgctcag caacctgccg
     4741 gcgtcggcgt cggtcacctg gatgcgtccg ttcaaggagg tgacgatgat cgtgccgtcg
     4801 gtcttgaccg tgtcggcctc gtcgaccccg tcctcctggt tgttcgtgcc gctccgggcc
     4861 tggtccgcgg acgacccgga ccccgccgcc gcacctgtcg cgccggccga cggggtcgcc
     4921 ccttcgacgg ccatgccggc gttgtacgcg accggcccgc cggccagccc gtagggcccc
     4981 accaggggct ttgcctcgct gcggaagtag tcgagcaact ggtcgcagga ggcgaacggt
     5041 tgcagtgccg ccgccgggtc ggccgtgaac gccccgcccc cggcacggcc gaccgggccg
     5101 gcggtgccgc tgggtcccga tgtcccggtg cagcccgcca ccagggccag ggcggacccg
     5161 gcagccgcca cagcgaccgc ggctcggcgc aggggtgaga tgggacggga cgctccgggc
     5221 gagaccttca tggtcatcct ccgcgcactg gtggccggtc gttgaccagg cttggacgcg
     5281 gaggacgacc agatggttcc ggggcgctac gcggtcggag cggcctcggc tgcctcgtcg
     5341 gcctcgggct cgggccggtg gggcgcgaga tagagagcgg cgagcttggg caccgacaaa
     5401 ctggccgaga acggctggtt gtcccacggg gtcggctcgg cggtgacgat cacgcccgac
     5461 tgcgaggggg agccgtactc gtcgaagccg gaggtgtcca tgatgacctc ccactcgccg
     5521 gcatacggca gcccgaaacg cattccgtcc ctagcgtttc cgccgaagtt gatcagggcg
     5581 acgacggcgc tgtcgccgcc gcggtcgggg ttgccgaagc ggacgtatga cagcgtgttg
     5641 ccggcgttgt cgtgcgggtt gagccactcg aaaccggccg ggctggaatc ctgcgcccat
     5701 agcgcgtcgt gggccttgta gatctggttg agctccttga ccaggttgtg cacgcgatag
     5761 tgcgccgcgt ggtcgagcag ccaccagtcg agcccgtgcc cgtcggccca ctccgacggc
     5821 tgggcgaact cgcagcccat gaagagcagc tgcttgcccg ggtggctcca gatgtaggcg
     5881 aggtaggcgc gcagggtggc gagctggtca taccggtgcc cggggatctt gccgagcagc
     5941 gagcccttgc cgtgcacgac ctcgtcgtgg ctgatcggca ggatgtagtt ctcgctgtag
     6001 gcgtacatca gcgagaacgt cagcaggttg tggtggtact gccggtgcac ggggtcgcgc
     6061 tgcaggtagc gcagcgagtc gttcatccat cccatgttcc acttcaggcc gaaaccgagc
     6121 ccgccgctgg ccgtctcctt ggtgacaccc ggccaggagg tggattcctc ggcgaccgtg
     6181 acgatgccgg ggtgcagctt gtatgccgtg gcgttggtct cctggaggag cccgatcgcc
     6241 tcgatgttct cgcggccgcc gtattggttg ggcagccact cgccgctgcg gcgggagtag
     6301 tcgaggtaga gcatggaggc gaccgcgtcg acgcgcacgc cgtcggcgtg gaactcctcc
     6361 atccaataca gggcattggc gacgaggaag ttgcgcacct ccttgcggcc gaagtcgaaa
     6421 atgtgtgtgc cccagtcggg ttggtcaccg cgacgcgggt cggcgtgctc gtagagggcc
     6481 agcccgtcga agcgcgccaa cgcccactcg tcgcgcggga agtgcgccgg gacccagtcg
     6541 aggatgacgc cgataccggc ctggtgcagc ttgtcgacga ggtacttgaa gtcgtcggga
     6601 gtgccgaagc gggccgtcgg tgcgtagtag ccggtgacct ggtaacccca cgacgggccg
     6661 taggggtgct ccatcaccgg caggaattcg acgtgggtga agccgaggtc ctggacgtag
     6721 ttgaccaggt gctcggcgag ttcgacatag gtcaggccgg cccgccagga gccgagatgg
     6781 acctcgtaga tgctcatcgg gccggtatgc ggattggtct cccgcttgcg tgtctcccac
     6841 tccgaatccg cccactggta ggccgagtcg tgcaccaccg agccggtgcg tggcgggacc
     6901 tcggtgcgca gggccatcgg atcggccttg tcgcgttcga catcgtcggc gccgcggatg
     6961 cggaacttgt aggtcgagcc cgggccgacg ccggggacga agagttccca gacaccggag
     7021 ttgccgagca ggcgcatcgg gtggctgcgg ttgtcccagc cgttgaaatc accaaccaac
     7081 gcaacggctt tcgcgcgcgg cgcccagacg gcgaaggacg tgcccgtcac cgagcccatc
     7141 gggccgtcgt aggtgtgcac gtgcgcgccc agcacctgcc acagcttctc gtgccggccc
     7201 tcgttgatga ggaagcgatc gatatcgccg agggtcgggg cgaaccggta ggggtcgtcc
     7261 tgctcgtgct cgacgccgtc ctcccacgtg gtcaggatgc ggtagtcgag agcgttcgtg
     7321 gcgccggggc gcagcccgct ccagatcccg tccgattcgt ggctcagttc gctgatctcg
     7381 ccgtcctcga agcgcacccg cacccgctgg gcgagcgggc gcatgacccg gatgcgcaga
     7441 ccctcggcct cgatgtgctg cccgagcagg tcgtgcggct gctggtgtcg gccgttcgcc
     7501 agggaccagc gggcgccggg cagggcgctg ggaggagtgg gcatggtcag ttctccttgg
     7561 tgggtagggc cgtgggcagc aaccggtcga tggcggcgag cgggatggtg agccagtccg
     7621 ggcggttgcg ggcctcgtag accacttcat acagggcttt gtcgacgata taggccgaca
     7681 gcagcggccc cgcgtcgcgg gggtcgtgtc ccgattcctc ggtgtagccg gcgaggaaag
     7741 cctcctgggt cgccgtgacc cagtcgcggg cgctctcgcc acggttctgc tgctcgaccg
     7801 agccgccggc gtagtcgaag gagcgcagca tgccggcgat atcgcgcagc ggctggtcgg
     7861 tgcgattgcg ctcggccagc gggcgcagcg gctcgccctc gaagtcgagg agcacccatc
     7921 cgcgctcggg cacgtcgagg acctgtccga ggtggtagtc gccgtggatg cgctggaagt
     7981 cgggccacga cgcggcggcg gccgcggcat acagtccgtc gatgccgacc gcgtgggcct
     8041 gcagatcggg cacctcggcg agcgcggatc ggaaccgggt gcgcatctcc tcgatcgtct
     8101 cggcgatgcg gccctcgtcg gccgcctcgg ccgcctcggc cgggagggtg tcgcgcagga
     8161 tcgcgtgcac ctgggcggtg gcggcgccga gggtgcgggc ctgttcggtg aagtcctccc
     8221 cgcggccggc ggcgaccagc gcttcgcgcc aggcgtcctg cgctccgggc aggaactcct
     8281 ggacgaaggc caggtgcccg tgcgccgtgc tgtcgccgtc ggcgcccggc caggacccgg
     8341 ccaccgcgcc caggctggcg ggcacgcagg tgcagccggc ggaggccagt gccccctgca
     8401 ggatgacgtc ggggttgtcg ccgtcggcca atgcgcggaa gaccttggtg atcaccgggt
     8461 gctcggcgcc ggcctcgtcg accgcgatgg agatgatcga ggtgttcgac tgctccccgg
     8521 tcaggacccg agaactggtc agccgcaacc cattcggcgt tccggtgccg gtgacggtct
     8581 cctcgacggt gtcgttcgcg ctgctcgagg cggcctgcgc ctgtccggtg gcgagcgcga
     8641 agaggcgctc ggcgaagacg gggtcgtggg gcgcgtcata cacatagcgg gtgccgagca
     8701 cagagtgctc gaccaccccg acgagggcgg cggccgcgcc ggcgagaggg gccgaccggt
     8761 aggtcaaagg cacctgatag accaccggcg ggctgctgga gtcgtccgcg aggatgatcg
     8821 actcgatgcc gacctcgccc tcggggtcat cgagccgcca gctgaacagc cgccgcggct
     8881 gcggtgtgct gcctttgccg gcataccacc gttgggagcc gatccagccg gtgagcaact
     8941 ccagcttggt gggggtgatc gaggcgccga agtgcaggat cgccatcgac tcagccccgc
     9001 cgcacgtgga tgatgtgcac cggttcccac tccgggccca gccgcacata gttgcgctcc
     9061 ccccactgcc agtcggcgcc gctgatctcg tcgtgggcgg tgaagctgtc gccccagtcc
     9121 atgcccaggg ccggcatgtt caagctgacg atggaactgc gggtcgagtg cgggtcgagg
     9181 ttggcgacca cgatgatcgt gtccacctcg cccgagggca gcacccggtg cttggagaag
     9241 cagagcaggt tctcgtcggc cacggtgtgg aaggtgatat tgcgcagcca gtgcagcgcg
     9301 gggtgggcgc ggcggatctc gttgagccgg gtcagatagg gcgcgagggt ctgaccgctg
     9361 cgcgggccgc cctcctcgta gtccatccag cggcggtcct tgaactggta tttctcgttg
     9421 tcgatctgtt cctcggcgcc gggccgggcg acgtgctcca tcagctcgta gccggtgtag
     9481 atcccgtagg tcgggacgag cgtcgccgcc agcgccgccc ggaccttcca ggccgccggc
     9541 ccgccgaact gcatataggg cgtgaggatg tcgtgggtgg tgggccagaa cgacgggcgc
     9601 atgtatgccg cggcctcgcc cgccagttcc tcgacgtact cgcgcaggtc ccacggctgg
     9661 ttgcgccacg cgtaataggt gtagctctgc tggaagccga ccttgccgag cgtgtgcatc
     9721 atcgccggct tggtgaaggc ctcggccagc cagatgacat cggggtggtc cttggcgacg
     9781 tcggcgatca gccactgcca gaactcgacc ggcttggtgt gcgggttgtc gacccggaag
     9841 atcttgacgc cgtggtcgat ccagacctgc atgacccggc gcacctcggc atacgccttc
     9901 tccgggtcgt tgtcgaagtt cagcggatag atgtcctggt acttcttcgg cgggttctcg
     9961 gcgtaggcga tcgtgccgtc ggcccggtgc gtgaacaact ccgggtggct ctggacccac
    10021 gggtggtcgg gggaggcctg cagcgcgatg tccatcgcca cctcgaggcc gtgccggccc
    10081 gcctcggcga cgaagaagtc gaagtcgtcg aaggtgccca ggtcggggtg gatcgcgtcg
    10141 tggccgccgt cgggcgagcc gatggcatag gggctgcccg ggtcgcgggg gccggcgtcc
    10201 agggtgttgt tggggccctt gcgggccgtg gtgccgatgg ggtggatcgg cgtcaggtac
    10261 acgacgtcga aacccatggc ggcgatggcc ggcaaccgct ccgtggccgt gcggagcgtg
    10321 cccgagatcc agccgcccgc gctctcgtcg tagtagcacc cctcggagcg ggggaagatc
    10381 tcgtaccacg cgccatacag cgcccgctcg cgctcgacga gcagcgggaa atcggccgtg
    10441 ggggaaacgt attcgcgcat cgggagccgg tccatctcgg cgtgcacgtc gggatggacg
    10501 ccgccgtgca ggcgcaccgc cggctccagc cgcgagtcgc gcaggcggga aacggcctcg
    10561 tcgagaagca ggcggccctc gccgctgcgg cccggctggg ccgccgcccg ctccaacagc
    10621 agcgcgccct cggtgagcat caattcgaca tcgacgccgg cggccacctt gatggtcgcg
    10681 tcgtgttccc acgtgccgaa cggatccccc cagccctcca cccggtaggt ccagtcgccg
    10741 accgcgtcgg gggtgacctc ggcgatccac aggttcaggc cggggttgat gcagtccatg
    10801 ggcaggtgct gcgtggaccc gtcgggccgg gtcaggacga cggtcgcgtt gacggcgtcg
    10861 tgaccctcgc gaaagaccgt ggccgagatc gtgaacgtct ccccgaccac cgccttcgcc
    10921 ggccgggcac ccccgtcgac cagcggcttg acgtcctgga cgggtatgcg gccgatcggt
    10981 gtctgcgggg gcgtcgtcac gggggctcgc ggggtgctcg gttgggctgt ccgggtggcc
    11041 ggtcgagtca ggggagtcac ggcgcaaacc tatccaacgg aggcccacac ttgcaggctg
    11101 catggcgccg ccttgacctg gtcgccgaag ccgcgctcgt cctcccggct caggtcggcc
    11161 tcctcggcgg ccgaatccca catcagccgg tagcgctgcc gtcccgggat gtcgggcagc
    11221 gtcatctgga ccgggtgggt ctgcccgttg acgatcagga ggatgtcggt ctcggcggca
    11281 ttggccgagc cgagcagcat cgagaagctg cgcgtggccg ggtcctccca ctcctgggcg
    11341 ccgagcggcg tgccctcggg cgagcgccac gacacgtcgg ccggcccgcc ctcgtatacc
    11401 gggtcgccgg tcaggaagcg ccgctggcgc aggacggcgt gctcgcggcg cagccgggtc
    11461 agcgccgcca cctggtcgac gagttcggcc tgccacggct cccagcgcca gtccagccag
    11521 ctgatctcgt tgtcctggca gtaggcgttg ttgttgccgt actgggtgcg tccgaactcg
    11581 tcgcccgcgc acaacatcgg caccccgctg gagatcagca gggtgccgag gaggttgcgg
    11641 atgctgcggc gccgggcggc gttgaccgct tcgtcgtcgg tggggccctc gacggtgtgg
    11701 ttccaggacc ggttgtgcga cgcgccgtcg cggttgcctt cgccgttggc ctcgttgtgc
    11761 ttctcgtcgt atgccgtcag gtcggccgcc gtgaaaccgt cgtgggccgt cacgaagttg
    11821 atcgacgccg tcggtgcgcg atggccgtgg ccgaagaggt cctgggaccc ggccagccgg
    11881 gtggccagca tgcgggaccc gtggccgggg ccaccgccga ggacgctcgc gacgtccgag
    11941 agccagaagg tgcgcacgtc gtcgcggaag cggtcgttcc actcgctgaa gggcggcggg
    12001 aactggccgg tgcgccagcc gccgaaaccg atgtcccacg gctccgccac gagtttggtg
    12061 gtggagagca gcgggtcggt ccgcagggcg acgaggaagg ggtggtccgg gtcgtagtcg
    12121 ccgttcttgt cccggccgag agcgacggcc aagtcgaacc ggaagccgtc gacgtgcatc
    12181 tcgcccaccc aatagcgcag tgagtccagc acgagctggc aggccaccgg gtggcacagg
    12241 tcgaaggtgt tgccgcaccc gctcatgtcg acatcgcgcc cgaaggcgtc gatgcggtag
    12301 taggcggcgg cgtccaggcc gcgccaggag agggtcggcc cgtcgatgcc ctgctcggcc
    12361 gtgtggttgt acacgacatc gaggatcact tcgatgccgc cggcgtgcag ggctcgcacc
    12421 atggccttga actcgtcgac gaccccctgc gggtcggccg cggcggcata ccgcccgtgc
    12481 ggagcgaaga agccgaggga gttgtaaccc caatagttga tcaaaccccg ttgcagcaga
    12541 gcgggttcgc tgacgaaggc gtgcacgggc agcagttcca gcgtcgtcac gccgatgcgg
    12601 tgcaggtgct cgatgagcgc ggggtggccc aggcccgcgt aggtcccgcg caggtgcgac
    12661 ggtatgccgg ggtgcagcat cgtcaggccg cggacgtggc cttcgtagag gaccgaactc
    12721 gaccaggggt gccgcggcgg cacgtcgccg ccccagtcga acgtggggtc gatcacgacc
    12781 gcggagccgc cgaagtccag ggcgcgggcg taagggtcca gga
//
LOCUS       DAOPJG010000090        12562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5283_length_12562_cov_8.217078, whole genome shotgun sequence.
ACCESSION   DAOPJG010000090 DAOPJG010000000
VERSION     DAOPJG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12562
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_12562_cov_8.217078"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..996)
                     /locus_tag="PKU75_09375"
     CDS             complement(217..996)
                     /locus_tag="PKU75_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPF81129.1"
                     /translation="MTSGLRWFACGEDAIPVGEEWLVARERERLSRMRFTKRRNEYLL
                     RRCAGKRAVAAALGLATDPAGLARIGMLNAPGGAPYVEVDGGRPGLEVSLTDRAGQAV
                     ALVGPEGALASGTIGIDLEIVETRSEGFVADFLTAPEQEYVAAERARAGSDGWDAAAN
                     LVWSAKEAALKVQRVGLRADTRSVVVTVVPVARDDGWGELAIAAASGEQYAGWWRRDG
                     AFLLTIATLGAADPPAPLPGSADLAAAEPVHSWLAHPVVGA"
     gene            1108..2142
                     /locus_tag="PKU75_09380"
     CDS             1108..2142
                     /locus_tag="PKU75_09380"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPF81130.1"
                     /translation="MTPSTMRAVVYDQPREFTVKEVPLRDPEPGEVRLKVVQGGVCGT
                     DLHLHEGGFITSYPLTPGHENVGTVEALGDGVDSFRVGELVAVNPNTTCGKCPACRSG
                     DRLYCPSFGGWGTLKPGGFADYLIVPEEQVFSAEGLDPDVAVFAEPAACAVHGIEILQ
                     PRPGTTALVFGTGPTGLLLAQLLASSGAAHVTVASIAEPQLRRAEELGLDSAYLMVRG
                     RLDDDIAELAKRGDGGFDYVIDATGVAQVQERTVELARSGGTVLLYGVAHEDDRIAIS
                     PYEVFRRELTIRGSFAEIDTVPAALAALRSGRIRTDGLITHRFGLDDYGAAMDAVRSD
                     DSAHKVVMTP"
     gene            complement(2214..>3259)
                     /locus_tag="PKU75_09385"
     CDS             complement(2214..>3259)
                     /locus_tag="PKU75_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPF81131.1"
                     /translation="VNQQAGYLNRQGVKAIVVLIHQGGTQEPPTSDYDGCTGFNGPIA
                     EMAPRFSSLVDVVVSGHTHQPYVCTQRDPQGRPRLVTSAASYGQMVTETNFTIDSRSR
                     DINRNSFVSHNVLVDRATTPDPAETAVIDKWKTLAGPIAATVVGTVAQDVTGDSSGNR
                     GIETPMADLIADSILWGTQSGNSQIALMNVGGVRASFLVNQISNGEAPGKITYTEAYN
                     VAPFGNLLVTMDMTGQQIKDTLEQQYDPTRGRQYLALGVSEGFSYTWDDSRPQGSKVV
                     AGSMTLNGAPMSMTATYRVGTLNFLANGGDNFTGFMSGTNLLGGKEDLQNLVDYLGTH
                     PGITPPPDRVSGL"
     assembly_gap    3260..3359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3360..3810)
                     /locus_tag="PKU75_09390"
     CDS             complement(<3360..3810)
                     /locus_tag="PKU75_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81132.1"
                     /translation="MRKSTTLLATGAVAALAATMAGTTGATAAAGDGARAIPVQLLSF
                     NDYHGHLEATDGPLASDPAETPAGGVEYLSAKLDQLRAKVGDANSLTVAAGDTIGGST
                     FLSGMFHDEPSVESLNTLGLDVTSVGNHEFDEGTAELLRMQRGGCHPV"
     gene            complement(3927..6074)
                     /locus_tag="PKU75_09395"
     CDS             complement(3927..6074)
                     /locus_tag="PKU75_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPF81133.1"
                     /translation="MSEHDDATTPHTPEQPPTAPRRAFERTHHGQTTSDPYAWMRDRE
                     DPELIAYLEAENSYADARTAHLEPLREKLFQEIKSRVQETDLSVPIGDGPWWYYGRTI
                     EGQQYAVHARCPRPGPSAPRPALEPGEIPDGEQVLLDGNAAAAGHEFFSLGVLSVSPD
                     HARLAAGIDLEGDERFDITITDIATGEILDQAITGAGYGGEFDNSGRYFFYVVVDDAW
                     RPYQVWRHEVGTPREQDVKVYQEDDERFWLGLGGSRDDSHLVIDLATKTTTESWILPA
                     DQPLGDWRLVRERVDGVEYGIEIAGETILIVHNIRNREGDLAWTDLADPGEWLPVATS
                     GPGERFLGVDAFDDFAVLTLRAEGLPTLRVLPRDAATESGFGEPLPWPGAGELTAVHL
                     AENPEPATTELVITEESFIHPRATYAVQPLNGGRTLLKRQPVLGDFDPADYEESREWV
                     QTVDGARIPVSIVRRAGLEPVGLAPGLLVGYGAYEISSDPYFSVARLSLLDRGVVYAV
                     AHVRGGGELGRGWYEDGKLAAKTNTFTDFVAVADDLRGRWVHIDRLACEGGSAGGLLI
                     GAALNLSPQSFRAAHAAVPFVDALTSMLDPDLPLTAGEWEEWGNPLADPAAYDTMAAY
                     APYDNVRATAYPAILATTSLHDTRVLVTEPAKWIARLREVATNDPVTRPILLHTEMSA
                     GHGGRSGRYDAWREVAWEWAWLLDQLGAVETVH"
     gene            complement(6103..9678)
                     /gene="dnaE"
                     /locus_tag="PKU75_09400"
     CDS             complement(6103..9678)
                     /gene="dnaE"
                     /locus_tag="PKU75_09400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPF81134.1"
                     /translation="MSTPASASASRDNFVHLHVHTEYSMLDGAARIDELLKTAADQGM
                     PAIATTDHGYIYGAYEFWSKARKYDIKPIIGVEAYITPGTPRGVKERVKWGDGGRDDV
                     GGNGAYTHMTLLSRTTEGMHNLFRMSSLASLEGQYYKPRMDRELLAQYSTGLIGTTGC
                     VGGEIQTKLRFGLYEEAKQAAGELRDIFGTEHFYAEVMDHGIPIERQTQAELLRLAKE
                     MGLPLLATNDLHYTKADDAKAHAALLCVQSASTLMDPGRFKFDADNFYLRSAAEMRQL
                     FRELPEACDNTLKIAEMCEVSFVEKEGQYMPRFPCPPGEDETSWFIKEVETGLQARYP
                     GGVPEASRKQAQYEIDIIVGKGYPGYFLVVADFINWAKDNGIRVGPGRGSGAGSMCAY
                     AMRITDLDPLKHGLIFERFLNPERPSMPDFDVDFDERRRGEVIKYVTEKYGDDRVAQI
                     VTYGTIKAKQAVKDAARVLGHPFAMGDRLTKAMPADVMGKGVPLKEIFNAEHPRYGDG
                     ADFRTIHEAEEPVREIVDTAIGLEGLKRQAGVHAAGVIMSSEPLTDLIPIMKREQDGQ
                     IITQFDYPSCEQLGLVKMDFLGLRNLTILDDAIDNIRDNRGEEIDLDRLSETLDDPAT
                     YQLLARGDTLGVFQLDGGGMRTLLKLMQPDNFEDISAALALYRPGPMGVDAHTNFALR
                     KNGKQKEVPLDPRLDGQLEPKMIEALQPILGTTYGLCVYQEQVMELAQKLAGYTLGSA
                     DLLRRAMGKKKKEVLDAEFGTFSAGMRSQGFSQGSVKALWDVLVPFSDYAFNKAHTAA
                     YGLVSYWTGYLKANYPAEYMAALLTSVRDDKDKSALYLNESRRMGITVLPPSVNESRA
                     AFAAVGSDIRFGMEAIRNVGRNVVDAIVAAREEKGKFTSFSDFLEKVPASVCNKRTIE
                     SLIKGGAFDDLGHPRGGLLRIHEDYVDAFVEAKRAEAMGQDSLFGGLDEEFSADLSGL
                     PPIPQIAWDKRTQLGHERDMLGLYVSDHPLFGIEHVLIRHSDTGIAALLEEDSGHEDG
                     AQVVVAGLITSMAVKRNKKGDPYATVTVEDLGGAVEIMFFTKTYLACQHLLAMDAVCS
                     VKARVRRREDGVQLSAVDFQLPEITDGPRGPVVISMETTRATTPQIEQLKSVLASYPG
                     ATEVHLKLVQPGRSVLIKLDDRWRVDANEALMGDLKVLLGARSVS"
     gene            9942..12524
                     /locus_tag="PKU75_09405"
     CDS             9942..12524
                     /locus_tag="PKU75_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81135.1"
                     /translation="MARAGSRWLGGRSGAPWGDPQPIRAEIFSAERFEDHARSLADSH
                     TIAKHAERVVPLLTRLRADMAALTKSYEAIADDVQQGREITPAAEWLLDNFHAVETHV
                     RQIRLDLSPDYFEELPKLGPGFLSGHPRIFALMWGFVAHTDSLIEPDLLSRYVRAYET
                     RKALTLGELWAVAITLRLLLVENLRRVADLVVTAGRDRAAADTAADRLLGLDSANGLT
                     PDLLPQQRLASRAFTARLLQRLRGLENTEVREWLQSRVDTGQGEALMVAEAHSQSAAV
                     VTVRNIFTSLRLITDMNWEDWLESVSLIEEELRTHEGYLALDFPTRNLNRSAIEDLAR
                     RSGQEEIDVARAALQRARFAPPGPGRDVGFWLFDRGRAEFQKAIDYRPTLRRRLVDLV
                     RRLGLPGYLTAVGATTAVLLAFAALVTTFVLPGRTWPSWPLTALLAAVTALAVSDFAV
                     TVVNYWTSRLVSARPLPSLALRDGVPEELRTLVVIPAMLSSAAEVDELAGQLEVHHLA
                     NPDGELYYGLVVDWLDAPEPMVEGDQELLDRARRRIGALNAEHGDRFLLFHRPRRFNP
                     AEGVWMGWERKRGKLDELNALLRGRDSGLTVAEGRLPGPFRYVITLDSDTRLPREAAR
                     RLIGKLAHPLNQAHLHPSGRFVSRGYGILQPRITPALPMAEDSSLLQRIYSTRRGADR
                     YAFAVSDAYQDLFGQGSFTGKGIYDIDIVDRLITHRIPENRVLSHDLLEGSYARSGLV
                     TDIEVVEEHPTAYAVMASRIHRWTRGDWQLLGWIFGRRGVDALGRWKMADNLRRSLVP
                     IAQVAMLVLALLLFPAAVAAVWLALLMALHISPHVVLTLPRFLLRRKGVTARSQLSGA
                     LGDC"
BASE COUNT         1778 a         4385 c         4281 g         2018 t
ORIGIN      
        1 gcggggccag gttcgactga agcggggcca ggttcgactg aagcggggcc aggttttccg
       61 acgggggccg ggttatagcc cggcccccgt cggaaaacca ggccccgctt gctctccggc
      121 ccacttcact cccgcttcag tccggtccgc tgggtcgccg gccgcccctc aggatgcctg
      181 acgaaccgtt ctcgttgtcg ccgcgacgcg cccgcgctag gcgccgacca ccgggtgcgc
      241 gagccagctg tggaccggct cggccgcggc caggtcggcg ctgccgggca gcggggccgg
      301 cgggtcggca gcaccgaggg tggcgatcgt cagcaggaac gcaccgtccc ggcgccacca
      361 gccggcatac tgctcccccg aggcggcggc gatcgccaac tccccccagc cgtcgtcgcg
      421 ggccaccggc acgacggtga cgacgacgct gcgggtgtcg gcgcgcaggc cgacgcgttg
      481 caccttcagg gccgcctcct tcgccgacca gacgaggttg gcggcggcgt cccagccgtc
      541 gctgccggcc cgggcgcgct cggccgcgac atactcctgc tcgggcgccg tgaggaagtc
      601 ggcgacgaag ccctccgagc gcgtctcgac gatctccagg tcgatgccga tggtgccgct
      661 ggcgagcgcc ccctccggcc ccaccagcgc gaccgcttgg ccggcccggt cggtgaggga
      721 cacctccagc cccgggcgcc cgccgtccac ctcgacatag ggcgcacccc cgggcgcgtt
      781 gagcatgccg atccgggcca ggccggccgg gtcggtggcc agtccgagcg cggcagcgac
      841 cgcccgcttg cccgcgcacc ggcgcaggag gtactcgttg cggcgcttgg tgaatcgcat
      901 gcgggacaag cgttctcgct cgcgcgccac cagccactcc tcgccgacgg ggatcgcatc
      961 ttccccgcag gcgaaccagc gcaggcccga ggtcatggca ccaccctggc accgattccc
     1021 ggccgtggcg agcgcgagca cccggcgcca tgggtgccgc acgggcggcg tggcgcggga
     1081 tgccggctgt ggaggatgct tggtgccatg acaccctcga cgatgcgtgc cgtggtctat
     1141 gaccaacccc gcgagttcac ggtcaaggag gtgccgctgc gcgacccgga gccgggcgag
     1201 gtgcggctca aagtggtcca aggcggcgta tgcggcaccg acctgcacct gcacgagggc
     1261 gggttcatca cgagctatcc gctgaccccg ggccacgaga acgtcgggac cgtcgaggcg
     1321 ctgggtgacg gggtggacag cttccgggtg ggtgaactgg tcgccgtcaa ccccaacacg
     1381 acctgcggca agtgcccggc ctgccgatcc ggcgaccgcc tctactgccc gagcttcggg
     1441 ggatggggca cgctcaagcc gggcggtttc gccgactacc tcatcgtccc cgaggagcag
     1501 gtgttctcgg ccgagggcct cgatcccgac gtcgccgtct tcgccgaacc cgccgcctgc
     1561 gccgtccacg gcatcgagat cctgcaaccc aggccgggca ccaccgccct cgtcttcggg
     1621 accggcccga ccggcctgct cctggcccaa ctcctcgcct cctccggcgc cgcccacgtc
     1681 accgtcgcct cgatcgccga gccgcaactg cggcgcgccg aggaactcgg cctggactcg
     1741 gcatacctca tggtgcgcgg gcgcctcgat gacgacatcg ccgagctggc gaaacgcggg
     1801 gacggcggct tcgactacgt catcgacgcg accggcgtcg cgcaagtcca ggagcgcacc
     1861 gtcgaactcg cccgctccgg tggcacagtg ctgctttacg gcgtcgcgca cgaggacgat
     1921 cggatcgcga tctcgccgta tgaggtgttc cgccgcgagc tgacgatccg cgggtccttc
     1981 gccgagatcg acaccgtgcc cgccgccctg gcggcgctgc gcagcggccg catccgcacc
     2041 gacggcctga tcacccaccg gttcggcctg gacgactacg gcgcggcgat ggatgcggtc
     2101 cgtagcgacg acagcgccca caaggtcgtc atgacgccct gaccgcccga gacaccggcg
     2161 aaggccccgc cggcacgcag ccggcggggc cttcggagaa cttcgtgggt atttcagagt
     2221 ccggagacgc ggtccggcgg cggggtgatg ccggggtgcg tgccgaggta gtcgacgagg
     2281 ttctgcaggt cttccttgcc gccaagcagg ttggtgcccg acatgaaccc ggtgaagttg
     2341 tcaccgccgt tggcgaggaa gttcagcgtg ccgacgcggt aggtcgccgt catgctcatc
     2401 ggcgccccgt tgagggtcat tgagcccgcg acgaccttgc tgccctgcgg ccggctgtcg
     2461 tcccaggtgt agctgaaacc ctcggagaca ccgagcgcga gatactggcg gccacgggtc
     2521 gggtcgtact gctgctcgag ggtgtccttg atctgctggc ccgtcatgtc catcgtcacc
     2581 agcaggttgc cgaagggcgc gacgttgtag gcctcggtgt aggtgatctt acccggggcc
     2641 tccccattgc tgatctggtt caccaggaag gacgcgcgca ccccgccgac gttcatcaac
     2701 gcgatctggc tgttgccgct ctgggtgccc cacaggatgg agtcggcgat caggtcggcc
     2761 atcggggtct cgatgccccg gttgccgctg gagtcgccgg tgacgtcctg ggcgaccgtg
     2821 ccgaccacag tggcggcgat cggaccggcg agggtcttcc acttgtcgat caccgcggtc
     2881 tccgccgggt ccggggtggt ggcccgatcg acgaggacgt tgtgggagac gaaggagttg
     2941 cggttgatgt ccctcgaccg ggagtcgatc gtgaagttgg tttcggtgac catctggccg
     3001 tatgaggccg cgctggtgac caggcgcggc cgcccttgcg ggtcgcgctg cgtgcagacg
     3061 tagggctggt gggtgtgacc ggagaccacg acgtcgacga gcgaagagaa ccggggtgcc
     3121 atctcggcga tgggaccgtt gaagccggtg cagccgtcgt agtccgacgt cggcggctcc
     3181 tgcgtgccgc cctggtggat cagcacgaca atggccttga ccccctggcg gttgaggtag
     3241 ccggcctgct ggttgaccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3361 gaccggatgg cagccgccgc gctgcatccg cagcaactcg gccgtgcctt cgtcgaattc
     3421 gtggttgccg acgctcgtga cgtccaggcc gagggtgttg agggactcga cgctcggctc
     3481 gtcgtggaac atgcccgaca agaacgtcga gccgccgatg gtgtcaccgg cggcgacggt
     3541 cagcgagttg gcgtcgccga ccttggcccg cagctggtcg agcttggccg agagatactc
     3601 gacaccgccg gccggggtct cggccgggtc gctggcgagc gggccgtcgg tggcctcgag
     3661 gtggccgtgg tagtcgttga acgagagcag ctgcaccggg atcgcccggg caccgtctcc
     3721 cgcagccgcc gtcgcgccgg tggtgccggc catcgtcgcg gcgagcgccg cgaccgcacc
     3781 ggtcgcgagc agcgttgtgg acttgcgcat gaaaaaacct ccctaaggag cggccgaacc
     3841 tcccgagccg ccgggaggca acctacccgc ggacgtggac gagaaccacc cccgggtggc
     3901 tgtgaatcgt aaacactcgg tgactttcag tggacggtct cgacggcgcc gagctggtcg
     3961 agcagccagg cccactccca cgcgacctcg cgccacgcgt cataccgccc gctgcgcccg
     4021 ccgtggcccg ccgacatctc cgtatgcagc aggatcggcc gggtgaccgg atcgttggtc
     4081 gcgacctcgc gcagccgggc gatccacttg gccggctcgg tcaccaggac gcgggtgtcg
     4141 tgcagggacg tggtggccag gatcgccggg tatgccgtgg cgcgcacgtt gtcgtagggc
     4201 gcgtaggcgg ccatcgtgtc gtatgccgcg gggtcggcca gcgggttgcc ccactcctcc
     4261 cactcccccg ccgtcaacgg caggtcgggg tcgagcatgg aggtgagcgc gtcgacgaag
     4321 gggaccgcgg cgtgggcggc gcggaagctc tgcggcgaga ggttgagggc cgcgccgatg
     4381 agcagcccac cggccgaacc gccttcgcac gcaaggcgat cgatgtgcac ccaccggccg
     4441 cgcaggtcgt cggcgaccgc gacgaagtcg gtgaaggtgt tggtcttcgc cgccagtttg
     4501 ccgtcttcgt accacccccg ccccaactcg ccgccaccgc ggacgtgcgc gacggcatac
     4561 accaccccgc ggtcgagcag actgagccgg gccacggaga agtaggggtc gctggagatc
     4621 tcgtaggcgc cgtagccgac gagcagcccc ggagcgaggc cgacgggctc gagacccgcg
     4681 cgccgcacga tcgagaccgg gatgcgcgcg ccgtccaccg tctgcaccca ctcgcgcgac
     4741 tcctcgtagt cggccgggtc gaagtcgccc agcacgggct ggcgcttcag caacgtgcgg
     4801 ccgccgttca gcggctggac cgcgtaggtg gcgcgggggt ggatgaagct ctcctcggtg
     4861 atgacgagtt cggtggtcgc cggctcgggg ttctcggcga ggtggaccgc ggtcaactcg
     4921 cccgcgcccg gccagggcag cggctccccg aagccggact ccgtggcggc gtcccggggg
     4981 agcacgcgca gcgtgggcag gccctcggcc cgcagcgtga gcaccgcgaa gtcgtcgaag
     5041 gcgtccacgc cgaggaaccg ctcccccggc cccgacgtcg cgaccggcag ccactcgcct
     5101 gggtcggcca ggtcggtcca cgcgaggtca ccctcgcggt tgcggatgtt gtggacgatc
     5161 aggatcgtct cgccggcgat ctcgatgccg tattcgacgc cgtcgacgcg ttcgcgcacc
     5221 agccgccagt cgccgagcgg ctggtcggcc gggaggatcc aggactcggt cgtcgtcttg
     5281 gtcgccaggt cgatgacgag gtggctgtcg tcgcgggacc cgccgaggcc gagccagaag
     5341 cgctcgtcgt cctcctggta gaccttcacg tcctgctcgc gcggggtgcc cacctcgtgc
     5401 cgccacacct ggtagggccg ccacgcgtcg tcgacgacga catagaagaa gtagcggccg
     5461 gagttgtcga actccccgcc gtagccggcg ccggtgatcg cctggtcgag gatctcgccg
     5521 gtggcgatat cggtgatggt gatgtcgaag cgttcgtcgc cctcgaggtc gatccccgcg
     5581 gccagccgcg cgtgatcggg gctgacgctc agcacgccga ggctgaagaa ctcgtgtccg
     5641 gccgcggcgg cgttcccgtc gaggaggacc tgctcgccgt cggggatctc gccgggttcg
     5701 agtgccggcc ggggcgcgct cggcccggga cgggggcagc gcgcgtgcac ggcatactgc
     5761 tggccttcga tggtccgccc gtagtaccac cacggcccgt cgccgatcgg caccgacagg
     5821 tcggtctcct gcacacgcga cttgatctcc tgaaaaagct tctcccgcaa cggttccaga
     5881 tgtgccgtgc gcgcgtccgc gtaggagttc tcggcctcta ggtaggcgat cagctcggga
     5941 tcctcccggt cccgcatcca cgcgtaggga tcgctggtcg tctgcccgtg atgggtccgc
     6001 tcgaacgccc gcctgggggc cgtcggcggc tgttcagggg tatgcggagt ggtcgcgtcg
     6061 tcgtgctcgc tcacccgatc accctatgtc cagctccctg tgtcagctca cgctgcgggc
     6121 accgaggagg accttcaggt cgcccatcag tgcctcgttg gcgtcgaccc gccagcggtc
     6181 gtcgagcttg atgagcaccg agcggccggg ctggacgagc ttgaggtgga cctcggtggc
     6241 gccggggtag gaggcgagca ccgacttcag ttgttcgatc tgcggcgtgg tcgcgcgcgt
     6301 ggtctccatc gagatgacga ccgggccgcg cggcccgtcg gtgatctcgg gcaactggaa
     6361 gtcgaccgcc gacaactgca ccccgtcctc acgccggcgc actcgcgcct tgaccgagca
     6421 gacggcatcc atggcgagca gatgctggca ggcgagatag gtcttggtga agaacatgat
     6481 ctcgaccgcg ccgccgaggt cctcgacggt cacggtggcg taggggtcgc ccttcttgtt
     6541 gcgcttgacc gccatcgacg tgatcaggcc ggcgacgacg acctgggcgc cgtcctcgtg
     6601 gcccgaatcc tcttccagca gagcggcgat gccggtgtcg ctgtgccgga tgaggacgtg
     6661 ctcgatgccg aagagggggt ggtcactgac atacagcccg agcatgtcgc gctcgtgccc
     6721 gagctgcgtc cgcttgtccc acgcgatctg cgggatgggc ggcagcccgg acaggtcggc
     6781 gctgaactcc tcgtcgaggc cgccgaagag cgagtcctgc cccatcgcct cggcccgctt
     6841 ggcctcgacg aaggcgtcga catagtcctc gtggatccgc agcagcccac cccgcgggtg
     6901 cccgaggtcg tcgaaggcgc cgcccttgat cagcgactcg atggtgcgct tgttgcacac
     6961 cgacgccggc accttctcga ggaagtcgct gaaggaggtg aacttcccct tctcctcccg
     7021 ggcggcgacg atcgcgtcga cgacattgcg cccgacattg cggatcgcct ccatgccgaa
     7081 gcggatatcc gagccgaccg ccgcgaaggc ggcgcgcgac tcgttgaccg acggcggcag
     7141 gacggtgatc cccatccggc gcgactcgtt gagatagagg gccgacttgt ccttgtcgtc
     7201 gcggacgctc gtcagcaacg ccgccatgta ttcggccgga tagttcgcct tgagatagcc
     7261 ggtccaatag gacaccaggc cgtatgccgc cgtgtgggcc ttgttgaagg cgtagtccga
     7321 gaagggcacc aggacatccc acagcgcctt gaccgagcct tgcgagaacc cctgcgaccg
     7381 cataccggcc gagaaggtgc cgaactcggc gtcgagcacc tctttcttct tcttgcccat
     7441 tgctcgacgc aacagatctg cgctgcccag cgtgtaaccg gccagtttct gcgccagctc
     7501 catgacttgc tcttgataga cacaaagtcc gtatgtggtg ccgaggatgg gctggagggc
     7561 ctcgatcatc ttgggttcga gctgcccgtc caggcgggga tcgagcggga cttccttctg
     7621 tttgccgttc ttgcgcaacg cgaagttggt gtgggcatcg acgcccatgg ggccgggccg
     7681 gtagagcgcg agcgcggcgg agatgtcctc gaagttgtcg ggctgcatca gtttcagcag
     7741 cgtgcgcatg ccgccgccgt cgagctggaa gacgccgagc gtgtcgccgc gcgccagcag
     7801 ctgataggtc gccgggtcgt cgagggtctc cgagagccgg tcgaggtcga tctcctcgcc
     7861 gcggttgtcg cggatgttgt cgatcgcgtc gtcgaggatc gtcaggttgc gcaggcccag
     7921 gaagtccatc ttgaccagcc cgagttgctc acagctcgga tagtcgaact gggtgatgat
     7981 ctgcccgtcc tgctcccgtt tcatgatcgg gatcaggtcg gtcagcggct cgctcgacat
     8041 gatcacgccg gccgcgtgca cgccggcctg ccgcttcagg ccctccagac cgattgcggt
     8101 gtcgacgatc tcgcgcaccg gctcctcggc ctcgtggatg gtgcggaagt ccgcgccgtc
     8161 gccgtagcgg gggtgctcgg cgttgaagat ctccttcagt ggcacgccct tgcccatgac
     8221 gtcggcgggc atggccttgg tcagccggtc gcccatcgcg aacgggtgcc cgagcacccg
     8281 ggcggcgtcc ttgaccgcct gcttggcctt gatcgtgccg taggtgacga tctgggcgac
     8341 ccggtcatcg ccgtacttct cggtgacata tttgatgacc tcgccccggc ggcgctcgtc
     8401 gaagtcgacg tcgaagtcgg gcatcgacgg ccgctccggg ttgaggaagc gctcgaagat
     8461 caggccgtgc ttgagcgggt cgaggtcggt gatccgcatc gcataggcgc acatcgaccc
     8521 ggcgcccgaa ccgcggcccg ggccgacccg gatgccgttg tccttggccc agttgatgaa
     8581 gtcggcgacg acgaggaaat agccgggata acccttgccg acgatgatgt cgatctcgta
     8641 ttgcgcctgt ttgcgggagg cctccggcac tccccccgga tagcgggcct gcaacccggt
     8701 ctcgacctcc ttgatgaacc acgaggtctc gtcctcccct gggggacaag ggaatcgcgg
     8761 catgtactgg ccttccttct cgacgaagga gacctcgcac atctcggcga tcttgagggt
     8821 gttgtcgcac gcctcgggca gctcgcggaa cagctggcgc atctcggcgg cgctgcgcag
     8881 atagaaattg tcggcgtcga acttgaaccg gcccggatcc atcagcgtcg acgccgactg
     8941 cacgcacaac aaggccgcgt gcgccttggc gtcatcggcc ttggtgtagt gcagatcgtt
     9001 ggtcgccagc agcggcaagc ccatctcctt ggccagccgc agcagttcgg cctgcgtctg
     9061 gcgttcgatg ggtatgccgt ggtccatcac ctcggcgtag aagtgctccg tgccgaagat
     9121 gtcgcgcagc tcgccggccg cttgcttggc ctcctcgtag aggccgaagc gcagcttggt
     9181 ctggatctca ccgccgacgc agcccgtcgt gccgatcagg ccggtcgagt attgcgcgag
     9241 gagctcgcgg tccatgcgcg gcttgtagta ctgcccctcg agcgaggcca gcgagctcat
     9301 gcggaacagg ttgtgcatgc cctcggtcgt gcgcgacagc agcgtcatgt gcgtgtaggc
     9361 gccattgccg ccgacgtcgt cgcggccgcc gtcgccccac ttcacccgct ccttgacccc
     9421 gcgcggggtg ccgggcgtga tgtaggcctc gaccccgatg atcggcttga tgtcgtattt
     9481 gcgcgccttg gaccagaact cgtaggcgcc gtagatgtag ccgtggtcgg tcgtcgcgat
     9541 cgccggcatc ccctggtcgg ccgccgtctt gagcagctcg tcgatgcgcg ccgcaccgtc
     9601 gagcatggag tactcggtgt gcacgtgcag gtggacgaag ttgtcccggg aagctgaggc
     9661 ggaggctggc gtcgacatcg gatctgcagt ctaagtcgcc cctccgacag ccctcggcgt
     9721 ggccgagccc gtgtcgccct cccatccctc ccgggaccgg cctgggtgtg cgagttcaca
     9781 cccgcagggg aagctggtgg cgcccggccg gcactagcct ggatcggaag gctggcgcgc
     9841 cgggtgcccc gagactctcg ggccaccgga accggcgcgt ccggtccgtc tctcccaccg
     9901 ggccgttgcc ttcgccgatg cgagtcgacg ggagaggtgg catggcgcgg gccgggtcca
     9961 ggtggctggg cggccggtcc ggcgcgccgt ggggcgaccc ccaacccatt cgcgccgaga
    10021 tcttctcggc cgagcggttc gaggaccacg cccgctccct ggccgacagc catacgatcg
    10081 ccaagcacgc cgagcgggtc gtgcccctcc tgacccggct ccgggcggac atggccgcgc
    10141 tgaccaagtc ctacgaagcc atcgccgacg acgtccaaca gggacgggag atcacgcctg
    10201 cggccgaatg gctcctcgac aacttccacg ccgtcgagac ccacgtgcgc cagatccgtc
    10261 tcgacctctc ccccgactac ttcgaggaac tgcccaaact cgggcccggt ttcctctctg
    10321 gccacccgcg catcttcgcc ctgatgtggg gtttcgtcgc ccacaccgac agcctcatcg
    10381 aacccgatct gctctcgcgc tatgtgcgcg cctacgaaac ccgcaaggcc ctgaccctcg
    10441 gcgagttgtg ggcggtcgcc atcaccttgc gcctgctgct ggtggagaac ctgcgccggg
    10501 tcgccgacct cgtcgtcacc gccgggcgcg accgcgcggc cgcggacacg gccgccgacc
    10561 ggctgctcgg actcgactcg gccaacgggc tcaccccgga cctcctcccc caacagcggc
    10621 tcgcgagccg ggccttcacc gcccgcctgc tgcaacgcct ccgaggcctc gagaacaccg
    10681 aggtccgcga gtggctgcag tcgcgggtcg acaccggcca gggcgaggcg ctgatggtcg
    10741 ccgaggcgca cagccagtcg gccgcggtcg tcaccgtgcg caatatcttc accagcctgc
    10801 ggctgatcac cgacatgaac tgggaggact ggctcgaatc ggtcagcctc atcgaggagg
    10861 aactgcgaac gcatgagggc tacctcgccc tcgacttccc gacccgcaac ctcaaccgtt
    10921 cggccatcga ggatctggcc cgccgctccg gccaggagga gatcgacgtc gcccgggccg
    10981 cgctgcagcg cgcccgcttc gcaccccccg gcccgggccg tgacgtcggc ttctggctct
    11041 tcgaccgggg ccgcgccgag ttccagaagg cgatcgacta ccgcccgacc ctgcgccgcc
    11101 gtctggtgga cctggtacgc cgcctgggcc tgccgggcta tctcacggcg gtcggcgcga
    11161 ccaccgccgt gctgctcgcc ttcgccgcgc tggtcaccac attcgtcctg cccggccgca
    11221 cctggccgtc ctggccgctg accgcgctcc tggcagcggt caccgccctc gcggtgagcg
    11281 acttcgccgt caccgtcgtc aactactgga cctcccggct ggtctccgcc cggcccctgc
    11341 ccagcctcgc gctgcgcgac ggggtgccgg aggagttgcg gaccctcgtc gtcatcccgg
    11401 ccatgctgtc ctcggccgcg gaggtcgacg agttggccgg gcagttggag gtccaccacc
    11461 tcgccaaccc ggacggggag ctctactacg ggctcgtcgt cgactggctg gatgcccccg
    11521 aacctatggt cgagggcgac caggagctgc tggatcgggc gcgccggcgc atcggcgccc
    11581 tgaacgccga acacggcgac cgtttcctgc tcttccaccg cccgcgccgc ttcaacccgg
    11641 ccgaaggcgt ctggatgggc tgggaacgca aacgcggcaa actcgacgaa ctcaatgcgc
    11701 tgctgcgcgg tcgggacagc ggcctgacgg tcgccgaggg ccggctgcct ggcccgttcc
    11761 gctatgtcat caccctcgac agcgacaccc ggctgccgcg ggaggcggcc cgccggctca
    11821 tcggcaaact cgcccacccg ctcaaccagg cccacctgca tccctcgggc cggttcgtct
    11881 cccgcggcta cggcatactg cagccccgca tcaccccggc cctgccgatg gccgaggaca
    11941 gttcgctgct gcagcgcatc tactccaccc gccgcggcgc cgaccggtac gccttcgccg
    12001 tctccgacgc ctaccaggac ctcttcgggc agggctcgtt caccggcaag gggatctacg
    12061 acatcgacat cgtcgatcgc ctgatcaccc accgcatccc cgagaaccgc gtcctgagcc
    12121 atgacctgct cgaaggcagc tatgcgcgct cgggcctggt gacggacatc gaggtggtgg
    12181 aggagcaccc cacggcgtat gccgtgatgg cctcacggat ccaccgctgg acccgcggcg
    12241 actggcagct gctcggctgg atcttcggcc ggcgcggcgt cgacgccctg ggccggtgga
    12301 agatggccga caacctgcgc cgctcgctcg tgccgatcgc ccaggtcgcg atgctggtgc
    12361 tcgcgctgct cctcttcccg gcggctgtcg ccgccgtgtg gctggccctc ctgatggccc
    12421 tgcacatctc cccgcatgtc gtgctgacgc tgccgcggtt cctgttgcgg cgcaagggag
    12481 tcacggcccg cagccagctt tcgggcgccc taggagactg ctgaacaagg tgcctggcgg
    12541 cgggtggatc gccaccagtg at
//
LOCUS       DAOPJG010000091        12495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5322_length_12495_cov_7.924357, whole genome shotgun sequence.
ACCESSION   DAOPJG010000091 DAOPJG010000000
VERSION     DAOPJG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5322_length_12495_cov_7.924357"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..950)
                     /locus_tag="PKU75_09410"
     CDS             complement(117..950)
                     /locus_tag="PKU75_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81136.1"
                     /translation="MPEPAPVSVPPAPLPSAPLPTAPEDGEGRRDATAYPVLSGTVSG
                     AALLLLCEAAGSVFVAAAVALGVFVLLWGWPAVVGSPEPAGTRLAIAVAAAAAYLSGA
                     LDPAGLEVRQLPVAIAAGLVAGFLAQLVRGDIHAGVVAALTAGGAGIALVAAGLAYAP
                     LPESRYADRPVAAAVAGIVASVVADLLPARMRGLRLLAACLLGAAGGAVAGGLLVPGA
                     DLLVAGLLGLAAGVVSLAVRITLGELPGARLHSAPPVIAAASLLAPGLLVYTFARLLL
                     G"
     gene            complement(1019..1258)
                     /locus_tag="PKU75_09415"
     CDS             complement(1019..1258)
                     /locus_tag="PKU75_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPF81137.1"
                     /translation="MRYWAGAKAATGVAEEALTGDFAAGATVAAIRAAAVARHPQAEA
                     VLAVCAVLVDGLAADPVAVVPAAATVEFLPPFAGG"
     gene            1360..2058
                     /locus_tag="PKU75_09420"
     CDS             1360..2058
                     /locus_tag="PKU75_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF81138.1"
                     /translation="MARLLLLTNALAPSAEVLPALALLSHQVRILPAEAAVLVDAPPA
                     DALLVDARRELAQARSLTRILRTTGTSVPVIAVLTEGGLAGVTADWGVDDVLLDTAGP
                     AEVEARLRLTVGRLSALAAPEEEAPITAGELVIDEGSYSARIGGRHLDLTYKEFELLK
                     YLAQHPGRVFTRAQLLQEVWGYDYFGGTRTVDVHVRRLRAKLGGDYELLIGTVRNVGY
                     RFVPEEPAGAGSRA"
     gene            2068..3006
                     /gene="mshD"
                     /locus_tag="PKU75_09425"
     CDS             2068..3006
                     /gene="mshD"
                     /locus_tag="PKU75_09425"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HPF81139.1"
                     /translation="MPPHPLPAPTPVVALTPEDQAAVRALARAAQEADGIRPLGERFL
                     LGLDEADPASPHLLMRAEGGEPTAYGRVLDGAGELFVAPVARRQGIGTRLLAALAEQG
                     TPAVWAHGDLPRTRAFARANGWRRVRLLHKMSRPLLPDDDAEIALPQGFSLRSFTPGD
                     EDAWLAVNGRAFADHPEQGRVSRTDLDRLLAQPWFDPAGLLLLVDDATGDLAGSHWTK
                     VDPQERAPGTDGRATAAGEVYVVAVDPAYHGRGLAVPLTAAGLAHLARRGLRAVVLYV
                     DGDNDRALRTYRRLGFTDLEVHAQYRRVPAAAKMEP"
     gene            3003..5276
                     /locus_tag="PKU75_09430"
     CDS             3003..5276
                     /locus_tag="PKU75_09430"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HPF81140.1"
                     /translation="MTAPAEHPTIGEPAGTGPEIDGGPLRDITVAATAHTRMPRRAPN
                     GRFIRMPGGFPSDTGLPDDRFLDREVSWLQFNERVLQLAADPDIPLLERARFLAIFSS
                     NLDEFFMVRVAGLKRRIATGIAVRSASGLEPREVLEKVSSVSTDLLAAQSRIFHDHVR
                     PDLEAEGISIVRWDQLSEPEHDRLADLFTANIFPVLTPLAVDPAHPFPYISGLSLNLA
                     VVLVNPKTGKEHFARVKVPEMLPRLVQVEAGDEVTVDELFHTRFVPLEDLISAHLEQL
                     FPGMDVVASFTFRVTRNEDLEVEEDDAENLLTALEKELTRRRFGPPVRLEVDETMDGH
                     VLELLVRELGVHPSEVYQLPAPLDLRALHIIADVDRSDLHYSRFVPRTNAELAPTESA
                     KPRDVFASIRKQDILLHHPYDSFSTSVQSFIEQAAADPKVLAIKQTLYRTSGDSPIID
                     ALIDAADAGKQVLALVEIKARFDEENNISWARKLEQAGVHVVYGIVGLKTHCKLSLVV
                     RQETEGLRRYCHIGTGNYNPKTARLYEDLGLLTCDPAVGEDLTRLFNQLSGLAPRSRF
                     TRLLVAPRTVRSGLIDLIEAEIDHAEEGRGGRIGIKVNSIVDEQLIDALYRASQAGVQ
                     VQVWVRGICALRPGVEGLSENIEVRSILGRFLEHSRVFTFGGGGEPVTFIGSADMMHR
                     NLDRRVEALVELGPRHSQEVRALFDRGMSDRYAHWKLGADGRWVRHHRGPDGTPLDDL
                     QSDLIAFYAKRRRKASR"
     gene            5285..6226
                     /locus_tag="PKU75_09435"
     CDS             5285..6226
                     /locus_tag="PKU75_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF81141.1"
                     /translation="MPALRAVPAAGTIPWRVVDGELQVLLVHRPRYDDWSWAKGKLDP
                     GEEAAVAAARETHEESALTVRLGRPLPEARYQIFDRDHTYATKEVSYWAATVTDATGT
                     LVNEIDEIAWLGPTEAHNRLDYARDQDQLLALVAMHRAGELDTWPLIIVRHATALPRS
                     KWAQKDWLRPLDARGRARAQALVPLLAAYGPLRVVSSSSTRCRDTLAPYARATATQVK
                     LREALTEEKFEKAPKKAIALVDKEIRRGKPAAICTHRPLLPALLERLRDHGATPDVRA
                     MYNEAAANGMEKGELIVAHVSGTGPAARIVAAERYAP"
     gene            complement(6255..7031)
                     /gene="pstB"
                     /locus_tag="PKU75_09440"
     CDS             complement(6255..7031)
                     /gene="pstB"
                     /locus_tag="PKU75_09440"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPF81142.1"
                     /translation="MAKRIDLSDLDIFYGNFKAVEGVNMTVEPRAVTAFIGPSGCGKS
                     TVLRTLNRMHEVIPGGRVEGKVLLDGQDLYGAGVDPVGVRRTVGMVFQRPNPFPTMTI
                     RDNVAAGLKLNGLKDKKKLDEVVEKSLKGANLWNEVKDRLDKPGAGLSGGQQQRLCIA
                     RAIAVSPQVLLMDEPCSALDPISTLAIEDLINELKEKYTIVIVTHNMQQAARVSDQTA
                     FFNLKATGQPGHLIEVDTTHKIFNNPSEKATEDYISGRFG"
     gene            complement(7070..7984)
                     /gene="pstA"
                     /locus_tag="PKU75_09445"
     CDS             complement(7070..7984)
                     /gene="pstA"
                     /locus_tag="PKU75_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPF81143.1"
                     /translation="MTAVPVQDSVTPHDAPQIDLSGRSGGRSLKNNIATVVMYLAFAI
                     AVIPLVWILVSVIGKGGDLLLDSAWWTHSQSGITVRKEGGGAIHAIQGTLLQGLVTAL
                     ISVPIGVLTAVYLVEYGRGKFAKAVSFMVDILTGVPSIVAGLFIYAVFMTTFGMTRAA
                     IFVCLALTLLMIPTIVRSTEEMLKLVPNELREASYALGVPKWKTIFSVVLPTAFSGIV
                     TGILLGLARVMGETAPLLILGPYTKAIASNLFSGNMATLPTMINQDRGEAITLPAHER
                     IWAAALTLILLILLLNLLGRFASRFSKV"
     gene            complement(7981..8988)
                     /gene="pstC"
                     /locus_tag="PKU75_09450"
     CDS             complement(7981..8988)
                     /gene="pstC"
                     /locus_tag="PKU75_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPF81144.1"
                     /translation="MQSITGVSAVDETPVDGRSPLESDRASTGRLGDRLFGGAALGSG
                     AIVVALVTLIGVFLLSQAIPALAKNEANFLTSTEWSVSGDHPRFGIARMLWVTVLSSA
                     MALAIAVPLGICVALFLTQYAPKWLARPAAATVDLLAAVPSIVYGLWGLRALGPTTDG
                     LQNFLRDNVGWFPLFKEASVSSFTTIFLAGIVLAIMILPIITALSREVFAQTPTTHKE
                     GALALGATKWEMIRTAVLPFGKPGVISASMLGLGRALGETVAVMILVSSLAAGTDWSW
                     SLFSGGETFASKIALGSGEFDTPQKTGAYIAAGLVLFILTFIVNAIARVVIERRKAFT
                     E"
     gene            complement(9095..10273)
                     /gene="pstS"
                     /locus_tag="PKU75_09455"
     CDS             complement(9095..10273)
                     /gene="pstS"
                     /locus_tag="PKU75_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HPF81145.1"
                     /translation="MTHRRRAAVLLAAAGSLALAGCGSDNNTETSSSSAAGSSSSASD
                     SAGSSSSDSASESASSGGEAACGSGTINAEGSSAQKNAFETVVAAYNEKCPDVTINYN
                     PSGSGTGIKQFYSGQVDFAGSDSALKTEEKDGVIESQKAEEQCGSPAWNLPMVIGPIA
                     VAYNVEGVDKLVLNAEVLSGIFQGEITTWNDAKIAALNSGVDLPDTAIKPFFRSDESG
                     TTENFTKYLKAAGGDAWKGEPAKTWTGKGEGKEKSAGVADAVGSTAGGITYVEMSYAK
                     DNSLAMAQIDNGSGPVELTSESIGKLIATAEQQGEGNDLALSLDYATQEAGAYPIGLV
                     TYEIVCSKNKDAAKAEAIKGFLNYFAGSEAQASIEEIGYAPLPAEIQTKVAAAVAALS
                     "
     gene            complement(10488..11483)
                     /locus_tag="PKU75_09460"
     CDS             complement(10488..11483)
                     /locus_tag="PKU75_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPF81146.1"
                     /translation="MDWLPTAIVIALAMGFNFTNGFHDAANSVATSVSTRALTPRVAL
                     LLAAAANLIGALFGTRVAQTVGSGIIDAPHGTTGLTICAAALIGAVSWNLMTWWRGLP
                     SSSSHALIGGLGGAALAAGASVHWSVVLERVVIPMLVSPALGLVVGYAVMTAILWIFR
                     DAAPGRIGRGFRLAQTVSASAVAFGHGMQDAAKTAGIVVLALTVGGFHNNVDESVPLW
                     VLALSAVVMSLGTYAGGWRIMRTLGARIIDLDPPRGFAAEVTAASILFVATALRAPIS
                     TTHAITSAIMGAGATRRVRAVRWAVAADIGWAWLLTFPGAGLLAAAAYLVLAAVT"
     gene            complement(11486..12142)
                     /locus_tag="PKU75_09465"
     CDS             complement(11486..12142)
                     /locus_tag="PKU75_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPF81147.1"
                     /translation="MSKSYVGSALVVFRLRPRNDEMFFELLVSASGLLVDGARLLSGL
                     CADGDAGRADMIARMHEVEHRADEISHALIRRVDTSFITPFDREDLHRLVSGLDDCMD
                     HMDAAVDFIDVHALAQLPPRAVAQVEIIARMAELTAAVMSGLRDPARVRDYVVEVNRL
                     ENEADRGYRRLRAELLNGDPMDVLRMLQNLDIVQELEAACNAFERVAHTVEGISLKEP
                     "
     gene            12223..12408
                     /locus_tag="PKU75_09470"
     CDS             12223..12408
                     /locus_tag="PKU75_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81148.1"
                     /translation="MMPDGRDPDTAEDDLQTESELLKGVRFAISGSAGDLDENAIDEA
                     LGVTPLISEGSDDSDTA"
BASE COUNT         2006 a         4385 c         4315 g         1789 t
ORIGIN      
        1 tggtgtactg agggacattg gtgtcgtgag ggggcattgg tgtagtgagg gggcattggt
       61 gcactgaggg ccccgcacat tgcccgctcg cgggccggcc gcgaggcgcg cggtggctag
      121 ccgagcagca gccgggcgaa ggtgtagacc agcaggccag gcgccaagag cgaggccgcc
      181 gcgatgaccg ggggtgcgct gtggagccgc gcgcccggca gctcgccgag ggtgatgcgc
      241 acggcgagcg agaccacccc cgcggccagc cccagcaacc cggcgacgag caggtccgcg
      301 cccgggacca gcaatccccc ggccaccgcg ccaccggccg cacccagcaa acacgcggcg
      361 agcagccgca gcccacgcat ccgggccggc agcagatcgg cgaccacgct cgccacgatg
      421 cccgccacgg ccgcggcgac ggggcgatcg gcataccggc tctccggcag cggcgcgtag
      481 gcgagcccgg cggccaccag agcgatgccg gcgcccccag cggtcagggc cgccaccacg
      541 ccggcgtgga tgtcgccgcg gacgagttgg gcgaggaagc cggccaccag cccggcggcg
      601 atggccacgg gcagctggcg cacctcgagg ccggccggat ccagggcccc cgacaggtat
      661 gccgccgcgg cggcgaccgc gatggccagc cgggtgcccg ccggttcggg cgagccgacg
      721 acggccggcc agccccacag caggacgaag acgccgagcg cgacggcggc ggcgacgaag
      781 accgaccccg cggcctcgca gagcaacagc agcgccgccc cggagaccgt ccccgacagg
      841 accgggtatg cggtggcgtc gcgccgtccc tccccatcct ccggcgcggt gggcagcggc
      901 gcgctgggca gcggcgcggg gggaacgctc accggggcgg gctcgggcac ggccgtcatc
      961 ctcccatgcc ccggggccgc cctggatgac gtgacatctc gacggggtcg aatcgacctc
     1021 agccgccggc gaagggcggc aggaactcga cggtggcggc cgcgggcacc acggcgacgg
     1081 ggtcggcggc gagcccgtcg acgaggacgg cgcacacggc cagcacggcc tcggcctgcg
     1141 ggtggcgggc gacggcggct gcgcggatcg cggccacggt ggccccggcc gcgaaatccc
     1201 cggtcagggc ctcctccgcg acaccggtgg cggccttggc ccccgcccaa taccgcacgc
     1261 tcacgacgtc gcggccgccg ccggcagcgc cgggcaggga gaggtccgcg acctggtcgg
     1321 aaaggactag atcgttcatc aacatctatc ctgcctcgta tggcaaggct gttgctgctc
     1381 accaacgcgc tcgccccgag cgccgaggtg ctgcccgccc ttgcgctgct cagccaccag
     1441 gtgcgaatcc tgccggccga ggcggccgtt ctggtcgacg ccccacccgc cgacgccctc
     1501 ctcgtcgacg cccgccgcga actcgcccag gcccgctcgc tcacccgcat cctgcggacc
     1561 accggcacct ccgtgccggt catcgccgtc ctcaccgagg gcgggctggc cggcgtcacc
     1621 gcggactggg gtgtcgacga cgtcctgctc gacaccgccg gcccggccga ggtcgaggcg
     1681 cggctgcgcc tgaccgtcgg ccggctgagc gcgctcgccg ccccggagga ggaagccccg
     1741 atcaccgcgg gcgaactcgt catcgacgag ggcagctatt ccgcccggat cggcggccgg
     1801 cacctcgacc tgacctacaa ggagttcgaa ctcctcaagt acctcgccca gcaccccggc
     1861 cgggtcttca cccgcgccca actgctgcag gaggtctggg gctacgacta cttcggtggc
     1921 acccggaccg tcgatgtcca cgtccggcgg ctgcgcgcca agctcggcgg ggactacgaa
     1981 ctgctcatcg gcaccgtccg caacgtcggc taccgcttcg tccccgagga gccggcgggc
     2041 gccggttcgc gcgcctgagc cggtcgtatg ccgccgcacc cgctccccgc gccgaccccg
     2101 gtcgtcgcgt tgacccccga ggaccaggcg gccgtgcggg cgctggcgcg ggccgcccag
     2161 gaggcggacg gcatacggcc gctgggggaa cgcttcctcc tcggtctcga cgaggcggac
     2221 ccggcgagcc cgcacctcct catgcgggcg gaggggggcg agcccacggc atacggccgg
     2281 gtcctcgacg gcgccggcga actgttcgtc gcccccgtcg cgcggcgtca gggcatcggc
     2341 acccgcctcc tcgccgccct cgccgagcag ggcaccccgg ccgtctgggc gcacggggac
     2401 ctgccgcgca cccgcgcctt cgcccgggcc aacggctggc gccgggttcg cctgctgcac
     2461 aagatgtccc ggccgctcct ccccgatgac gacgccgaaa tcgctctccc ccaaggcttc
     2521 tcactccgca gtttcacccc cggcgacgag gacgcctggc tcgcggtcaa cggccgcgcc
     2581 ttcgccgacc accccgagca gggacgcgtg agccgcaccg atctcgaccg gctgctggcg
     2641 cagccctggt tcgatccggc gggcctgctg ctgctcgtcg acgacgcgac gggcgacctc
     2701 gcgggctcgc actggaccaa ggtggatccg caggagcggg cgcccgggac cgacgggcgc
     2761 gccaccgccg cgggtgaggt gtatgtggtg gcggtcgacc cggcatacca cgggcgcggc
     2821 ctggcggtgc cgctgaccgc tgccggcctg gcccatctgg cccggcgcgg cctgcgggcc
     2881 gtcgtcctct atgtcgacgg cgacaacgac cgcgcgctgc gcacctaccg gcggctcggg
     2941 ttcaccgacc tggaagtgca cgcgcagtac cgacgcgttc cggcggctgc caagatggag
     3001 ccatgaccgc gcccgctgag catccgacga tcggggaacc ggccggcacc gggcccgaga
     3061 tcgacggcgg cccgctacga gacatcaccg tcgccgccac cgcccacacc cgcatgcccc
     3121 ggcgcgcccc gaacggccgc ttcatccgca tgccgggcgg cttcccaagc gacaccgggt
     3181 taccggacga ccggttcctc gaccgcgagg tgtcgtggct gcagttcaac gagcgcgtcc
     3241 tgcagctggc cgccgacccc gacatccccc tgctcgagcg ggcccgcttc ctcgcgatct
     3301 tctcctccaa tctcgacgag ttcttcatgg tgcgggtggc cgggctcaag cgccgcatcg
     3361 ccaccggcat cgcggtgcgc tcggcatcgg gcctggagcc gcgggaggtc ctcgagaagg
     3421 tctcgagcgt cagcaccgac ctgctggcgg cgcagtcgcg gatcttccac gaccacgtcc
     3481 gccccgacct ggaggccgag ggcatctcga tcgtccggtg ggatcagctg tccgagcccg
     3541 agcacgaccg cctggcggac ctgttcaccg ccaacatctt tcccgtcctg acgccgctgg
     3601 ccgtcgaccc ggcgcacccg ttcccctata tctccgggct ctccctcaac ctcgccgtcg
     3661 tcctggtcaa ccccaagacc ggcaaggagc acttcgcccg cgtcaaggtg cccgagatgc
     3721 tgccccggct ggtgcaggtc gaggccggcg acgaagtcac cgtcgacgag ctcttccaca
     3781 cccggttcgt gccgctcgag gacctcatct cggcgcatct cgagcaactc ttccccggca
     3841 tggacgtcgt cgcgagcttc accttccgcg tcacccgcaa cgaggacctg gaggtcgagg
     3901 aggacgacgc cgagaacctc ctcacagctc tggagaagga actcacccgc cgccgcttcg
     3961 gcccgcccgt gcggctcgag gtcgacgaga cgatggacgg acacgtcctc gaactgctcg
     4021 tgcgcgagct cggcgtccac cccagcgagg tctatcagct gccggcgccc ctcgacctgc
     4081 gcgccctgca catcatcgcc gacgtcgacc gttccgacct gcactactcc cgcttcgtgc
     4141 cccgcaccaa cgccgaactg gcgcccaccg agtcggccaa accccgggac gtcttcgcga
     4201 gcatccgcaa gcaggacatc ctgctccacc acccctacga cagcttctcc acgtccgtcc
     4261 agagcttcat cgagcaggcc gcggccgacc cgaaggtgct cgcgatcaag cagacgctct
     4321 atcgcacctc gggtgactcc cccatcatcg acgccctcat cgacgccgcc gacgccggca
     4381 agcaggtcct cgcgctggtg gagatcaagg cccgcttcga cgaggagaac aacatctcct
     4441 gggcccgcaa actcgagcag gccggggtgc acgtcgtcta tggcatcgtc ggcctgaaga
     4501 cccactgcaa gctctcgctc gtcgtccgcc aggagaccga ggggctgcgc cgctactgcc
     4561 atatcggcac cggcaactac aaccccaaga ccgctcggct gtatgaggat ctgggcctgc
     4621 tcacgtgcga ccccgcggtc ggtgaagacc tgacccggct gttcaaccag ttgtcggggc
     4681 tggcaccgcg cagccgcttc acgcgtctgc tcgtcgcccc ccgcaccgtc cgcagcgggc
     4741 tgatcgacct catcgaggcc gagatcgacc acgctgagga gggccgcggc ggccgcatcg
     4801 gcatcaaggt caactcgatc gtcgacgagc agttgatcga cgccctctac cgggcctcgc
     4861 aggcgggtgt gcaggtgcag gtctgggtcc gcggcatctg cgccctgcgc cccggtgtcg
     4921 aggggctgtc ggagaacatc gaggtccgct cgatcctcgg ccgcttcctc gaacactccc
     4981 gcgtgttcac cttcggcggc ggcggcgaac cggtgacctt catcggcagc gccgacatga
     5041 tgcaccgcaa cctcgatcgc cgggtcgagg cgctcgtcga actcgggccg cggcacagcc
     5101 aggaggtccg ggcgcttttc gaccgcggca tgagcgaccg ctacgcgcac tggaagctcg
     5161 gcgccgacgg ccggtgggtg cgccatcacc gcggccccga cggcaccccc ctcgatgacc
     5221 tgcagagcga cctgatcgcg ttctatgcca agcggcgccg caaggcgagc cgataggagc
     5281 ccccatgcca gccctccggg cggtcccagc ggcagggacg atcccctggc gcgtcgtcga
     5341 cggcgaactc caggtgctgc tcgtccaccg gccacgctat gacgactggt cgtgggcgaa
     5401 gggcaagctc gacccgggcg aggaggcggc cgtcgcggcc gcccgtgaga cgcacgagga
     5461 gtcggccctc accgtccgcc tcggccggcc actgccggag gcgcgctacc agatcttcga
     5521 tcgcgaccac acctatgcca ccaaggaggt gagctactgg gcggcgaccg tgaccgatgc
     5581 gacggggacc cttgtcaacg agatcgacga gatcgcgtgg ctcgggccga ccgaggcgca
     5641 caaccgcctc gactacgccc gcgaccagga ccagctgctc gccctcgtgg cgatgcaccg
     5701 cgccggcgag ctcgacacct ggccactgat catcgtgcgg cacgccaccg ccttgccccg
     5761 ctccaagtgg gcgcagaagg actggttgcg ccccctcgac gcccgcgggc gggcccgcgc
     5821 gcaggcgctc gtgccgctgc tcgcggcata cggaccgctg cgcgtggtga gcagttccag
     5881 cacccgctgc cgcgacaccc tcgcgccgta tgcccgcgcc accgccaccc aggtgaagct
     5941 gcgggaggcg ctgacggagg agaagttcga aaaggcgccc aagaaagcga ttgcgttggt
     6001 ggacaaggag atccgccgcg gcaaaccggc ggcgatctgc acccaccggc cgctcctgcc
     6061 cgccctgctg gagcggctgc gcgaccacgg cgccaccccc gacgtgcggg cgatgtataa
     6121 cgaggccgcc gccaacggga tggagaaggg cgaactgatc gtcgcccatg tcagcggcac
     6181 cggccccgcc gcccgcatcg tcgccgccga gcgctacgcc ccctgaaccc caaggaacct
     6241 ctgctagcag aggcttagcc gaagcggccg gagatgtagt cctcggtcgc cttctcggac
     6301 gggttgttga agatcttgtg ggtggtgtcg acctcgatga ggtgacccgg ctggccggtg
     6361 gccttgaggt tgaagaaggc cgtctggtcc gagacccggg ctgcctgctg catgttgtgg
     6421 gtcacgatga cgatcgtgta cttctccttg agctcgttga tcagatcctc gatcgcgagc
     6481 gtggagatgg ggtccagggc cgagcacggc tcgtccatca ggagcacctg cggcgagacg
     6541 gcgatggcgc gggcgatgca caaccgctgc tgctggccgc cggacaggcc ggcaccgggc
     6601 ttgtcgagcc ggtccttgac ctcgttccag aggttggcgc ccttgagcga cttctcgacg
     6661 acctcgtcca gcttcttctt gtccttgaga ccgttgagct tcagaccggc cgcgacattg
     6721 tcgcggatcg tcatcgtcgg gaacgggttg ggccgctgga agaccatgcc gaccgtgcgc
     6781 cgcaccccga cggggtcgac gccggcgcca tagaggtcct gaccgtcgag gagcaccttg
     6841 ccctccaccc ggccgccggg gatgacctcg tgcatgcggt tgagcgtgcg cagcacggtc
     6901 gacttgccgc agccggacgg gccgatgaag gcagtgaccg cacggggttc gacggtcatg
     6961 ttcacgcctt cgacggcttt gaagttgccg tagaagatgt ccagatcgct gagatcgatg
     7021 cgcttggcca tgtggtttca gatgcctgtc tggcttgtcg ggattggggt cagaccttgc
     7081 tgaaccggct ggcgaaccga ccaagcaggt tgagcaggag gatgaggagg atgagggtga
     7141 gcgccgcggc ccagatccgc tcgtgcgccg ggagcgtgat ggcctcgccg cggtcctggt
     7201 tgatcatcgt cggcagggtc gccatattgc ccgagaacag gttcgaggcg atggccttgg
     7261 tgtagggccc gaggatgagc aagggtgcgg tctcgcccat gacgcgcgcc aagccgagca
     7321 ggatgccggt gacgatgccg gagaaggccg tgggcagcac gacgctgaag atcgtcttcc
     7381 acttcggcac cccgagcgcg taagacgcct cccgcagttc gttgggcacg agcttgagca
     7441 tctcctccgt cgaacgcacg atggtgggaa tcatcaacag cgtcagcgcg agacagacga
     7501 agatggcggc gcgtgtcatg ccaaaggtcg tcatgaagac ggcatagatg aacaggcccg
     7561 ccacgatgga gggcacaccg gtgaggatgt cgaccatgaa gctgacggcc ttggcgaatt
     7621 tgccgcgccc gtattcgacg agatagacgg cggtgagtac accgatcggg accgagatca
     7681 gcgcggtgac cagaccttgg agcagcgtgc cctggatggc gtggattgcg ccgccgccct
     7741 ccttccgcac ggtgattccc gactgcgagt gggtccacca ggcggagtcg aggagtaggt
     7801 cgccgccctt gccgatcacg ctgacgagaa tccagaccaa cgggatgacg gcgattgcga
     7861 aggcgaggta catcaccacg gtggcgatgt tgttcttcag ggaccggcca ccggatcgac
     7921 ccgagaggtc gatctgtggg gcgtcgtgcg gagtgacgct gtcctggacg ggcacggcgg
     7981 tcattcggtg aaggccttcc tgcgctcgat gacgacccgg gcgatcgcgt tgacgatgaa
     8041 ggtcaagatg aagagcacca ggccggcggc gatgtaggcg cccgtcttct gcggggtgtc
     8101 gaactcgccg gaccctaggg cgatcttgga ggcgaaggtc tcgccaccgg agaagaggct
     8161 ccaggaccag tcagtgcccg ccgcgagcga ggacacgagg atcatgacag cgacggtctc
     8221 gccgagagct cggcccaggc cgagcatgga cgcggagatg acgcccggct tgccgaaggg
     8281 cagcaccgcg gtgcggatca tttcccactt ggtcgcgccg agcgcgaggg cgccttcctt
     8341 gtgcgtggtc ggcgtctggg cgaagacctc acgggagagg gcggtgatga tcggcaggat
     8401 catgatggcc agcacgatgc cggcgaggaa gatcgtcgtg aagctggaga cgctggcctc
     8461 tttgaacagc gggaaccagc cgacgttgtc gcgtaggaag ttctgcaaac catccgtcgt
     8521 cgggcccaag gcccgcaggc cccacaggcc gtagacgatc gacggcacgg cggcaagcag
     8581 gtcgaccgtt gccgctgccg gacgggcgag ccacttcggg gcgtactggg tcaagaagag
     8641 ggccacgcag atccccaggg gcacagcaat tgccagggcc atcgccgaac tcagcaccgt
     8701 gacccacagc atgcgggcga tgccgaacct cgggtggtcg ccactgaccg accactccgt
     8761 cgaggtcagg aagttcgcct cgttcttggc gagagcggga atggcctgcg agagcaggaa
     8821 gaccccgatc agggtgacca gcgcgaccac gatggctccg gacccgaggg cggcgccacc
     8881 gaagagccgg tccccgagtc gacccgtgct ggcacggtcg ctctcgagtg ggctgcgccc
     8941 gtcgactgga gtctcgtcga cggcggagac tccggtgatg ctctgcatca gcgtccgtcc
     9001 ttcctcaggt ccggggcagg ggatgggaac acgtctggaa gagggcgcac aggccggtca
     9061 ccccggacgc ggggtgaccg gccggcgcca gggatcagct cagggctgca actgcggcag
     9121 caaccttcgt ctggatctcg gcggggagcg gcgcgtagcc gatctcctcg atgctggcct
     9181 gcgcctcgga gccggcgaag tagttcagga agcccttgat ggcctccgcc ttggcggcat
     9241 ccttgttctt ggagcagacg atctcgtagg tgaccagccc gatcgggtag gcgccggcct
     9301 cctgggtcgc gtagtccagg ctgagcgcga ggtcgttgcc ctcgccctgc tgctcggcgg
     9361 tggcgatcag cttgccgatg gactcgctgg tcagctcgac cggaccggaa ccgttgtcga
     9421 tctgggccat cgccaggctg ttgtccttgg cataggacat ctcgacgtag gtgatgccac
     9481 ccgcggtcga gccgacggcg tcggcgacac cagccgactt ctccttgccc tcgcccttgc
     9541 cggtccaggt cttggccggc tcgcccttcc aggcgtcgcc accggcggcc ttgaggtact
     9601 tggtgaagtt ctcggtggtg ccggactcgt cggagcggaa gaacggcttg atggcggtgt
     9661 ccggcaggtc gacaccggag ttcagagcgg cgatcttcgc gtcattccac gtggtgatct
     9721 caccctggaa gatgccggag agaacctcgg cgttgaggac gagcttgtcg acgccctcga
     9781 cgttataggc caccgcgatc gggccgatga ccatcgggag gttccaggcg ggcgaaccgc
     9841 actgctcttc ggccttctgc gactcgatga cgccgtcctt ctcctcggtc ttgagggcgg
     9901 agtcggaacc ggcgaagtcg acctggccgc tgtagaactg cttgatgcct gtgccggagc
     9961 cgctcgggtt gtagttgatg gtgacgtccg ggcacttttc gttgtaggca gcaacgaccg
    10021 tctcgaaagc gttcttctgc gccgacgagc cctcggcgtt gatggtgccg gacccgcacg
    10081 cggcctcccc gccggagctg gcggattcgc tggcggagtc gctcgaggaa gagcccgcgc
    10141 tatcggacgc gctcgaggag gagccggcgg ccgaagagga cgaggtctcg gtgttgttgt
    10201 cggatccgca cccggcgagg gcaaggcttc ccgccgcggc gagaagcacg gcagcgcggc
    10261 gacggtgggt gatcttcacg gaagtgaccc tctctaggtc tgactgcgtg gggtgccgtt
    10321 cgcacccaca gccaggacgc tagaaagcct cgatggcgcg gcggctctgc ctcgttgaac
    10381 gggaggtgaa cagcatgcgg agaattgggt tcgagaggtg ttgcacacca ctgcggagac
    10441 ttatggtgga cgactcccgg ccccaggggg cgccgggtgc gcctgagtca ggtcaccgca
    10501 gccaggacaa ggtatgccgc tgccgccagc agcccggcgc cggggaaggt cagcagccac
    10561 gcccagccga tgtcggcggc gacggcccat cggacggcgc gcacgcggcg ggtggcgccg
    10621 gcgcccatga tcgccgaggt gatcgcgtgc gtcgtcgaga tcggcgcccg caacgcggtc
    10681 gcgacgaaca ggatgctcgc cgcggtgacc tcggcggcga aaccccgtgg tgggtccagg
    10741 tcgatgatcc gcgcccccag cgtgcgcatg atgcgccacc cgccggcata cgtccccagg
    10801 ctcatcacca cagcgctcag ggcaaggacc cacaggggta cggactcgtc cacgttgttg
    10861 tggaaccccc cgaccgtcaa cgccaggacg acgatgcctg cggtcttggc ggcatcctgc
    10921 atgccgtgac cgaaggcgac ggcggacgcc gaaaccgtct gcgccagacg aaaaccgcgg
    10981 ccgatccggc ccggtgcagc atcgcggaag atccacagga tcgcggtcat cacggcatac
    11041 cccacaacca accccagggc cggggagacg agcatgggga tgacgacccg ttccaacacc
    11101 accgaccaat gcacactggc gccagcggcg agcgcggcgc cgccgaggcc gccgatcagg
    11161 gcgtgactgg aggaggaggg caggccgcgc caccaggtca tcaggttcca ggagacggca
    11221 ccgatcaggg cggcggcaca gatggtcagg cccgtggtgc cgtgcggtgc gtcgatgatc
    11281 ccgctgccca ccgtctgcgc gaccctggtg ccgaacaggg caccgatgag gttggcggcg
    11341 gccgcgagga ggagggcgac ccgcggggtc agggcccgcg tcgagaccga ggtggccacc
    11401 gagttggccg cgtcgtggaa gccgttggtg aagttgaagc ccatcgccaa ggcgatgacg
    11461 atcgccgtcg gcagccagtc cacgctcacg gctccttgag gctgatgccc tcgacggtgt
    11521 gggccacccg ttcgaaggcg ttgcaggcgg cttcgagctc ctgcacgatg tcgagattct
    11581 gcagcatccg caggacgtcc atcgggtcgc cgttgagcag ctcggcccgc agccggcggt
    11641 agccgcggtc cgcctcgttc tcgagccggt tgacctcgac gacgtagtcg cgcactcggg
    11701 cggggtcccg caacccggac atgacggcgg cagtcagctc cgccatccgg gcgatgatct
    11761 cgacctgagc gaccgcacgc ggcggcaact gggccagcgc gtgcacgtcg atgaagtcga
    11821 cggccgcgtc catgtgatcc atgcagtcat cgagccccga gacgaggcgg tgcagatctt
    11881 cacgatcgaa cggggtgatg aaggaggtgt cgacgcgccg gatgagagcg tggctgatct
    11941 cgtccgcccg gtgctcgacc tcgtgcatcc gggcgatcat gtccgcccgg ccggcgtccc
    12001 catcggcgca cagcccgctc agcaagcggg caccgtcgac cagcaggccg gacgcggaca
    12061 cgaggagttc gaaaaacatc tcgtcgttgc gtggcctcag gcggaaaacc acaagcgcgc
    12121 tcccgacgta agacttggac aaccccgcca tcctagaggc tggccgaaag gcgcgactct
    12181 caaccgcctc cggggcgcct tggcgtccta gggttaggtc gcatgatgcc cgacggccga
    12241 gaccccgaca cagccgagga cgatctccag acggagtcgg aactgctcaa gggcgtgcgc
    12301 ttcgccatct ccggctcggc cggcgacctg gacgagaacg ccatcgacga ggccctcggc
    12361 gtgacccccc tcatctcgga gggatcggac gactccgaca cggcttgagg ccggatgggc
    12421 tctctcggcc gggcatgctg gtgggccccg tgggactcga acccacaacc tacggattaa
    12481 aagtccgcag ctctg
//
LOCUS       DAOPJG010000092        12334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5393_length_12334_cov_6.980129, whole genome shotgun sequence.
ACCESSION   DAOPJG010000092 DAOPJG010000000
VERSION     DAOPJG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5393_length_12334_cov_6.980129"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..1307)
                     /locus_tag="PKU75_09475"
     CDS             complement(381..1307)
                     /locus_tag="PKU75_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HPF81149.1"
                     /translation="MNRKIALTIPSVAALAAAGLVATPAQARPVAGDGATAAECAPAG
                     ARLAKGATARDWNSVSDAKAAEMEAKFRADARAAGVRLDGAQREPFDVTIPVFVNVIH
                     DGAKGKLSMTKINRSIKVLDDAYRFVGIDFALKGVTYVNNKAWYNNMGQSYEYDDAIK
                     AKLHRGGTNVLNIYSANLSGGLLGYATFPWEDAGTSRDGVVILDESVPGGTAAPYNEG
                     DTLTHEVGHWMGLWHTFQGGCKTPGDQVFDTPYEASPAFGCPVGRDTCKNRPGNDPVK
                     NFMDYSEDPCMNHFTNGQKWRMQRVWNTYRML"
     gene            complement(1468..1764)
                     /locus_tag="PKU75_09480"
     CDS             complement(1468..1764)
                     /locus_tag="PKU75_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HPF81150.1"
                     /translation="MSETPGLDAPAAPSRPSTSTATLERTEVVPELQEPGDHERFSHY
                     VRKEKILESALSGEPVVALCGKIWVPGRDPKKFPVCPLCKEIYDGLRDPQDGED"
     gene            complement(1788..2348)
                     /locus_tag="PKU75_09485"
     CDS             complement(1788..2348)
                     /locus_tag="PKU75_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPF81151.1"
                     /translation="MTIPEDLTGRLLVATPEIEDGIFCRSVVLVLHHGTDGAQGVVLN
                     KPLEAEVEAILPGWGAVATLPAMVFQGGPVQMDSALGLVSLGDLPPADSRLRRLFGSI
                     GLVDLDSDPQVISGQVCAVRIFAGYAGWSANQLEGEIVAGSWYVVEREPADAFAAGTD
                     LWRAVLARQPGPLAFVAAYPADPGLN"
     gene            complement(2411..2797)
                     /locus_tag="PKU75_09490"
     CDS             complement(2411..2797)
                     /locus_tag="PKU75_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81152.1"
                     /translation="MDNRGTARIGASAASRMPLPRLLVWVALIASLVVNVVLLFMPSR
                     GGEPLFPYDDKVIHCVLFGVPAYLGLRAGLKPAVWLPAMAGWAVLSEILQARLTATRS
                     GSVWDCCADLVGIGIAVLLGAGFRAR"
     gene            2838..3452
                     /locus_tag="PKU75_09495"
     CDS             2838..3452
                     /locus_tag="PKU75_09495"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPF81153.1"
                     /translation="MTGTGVASAPPDVVVLDTAVRTPGGSVAEALSRADTAMTAIVGI
                     ARERGLAPADLQTTSASVYPQYDREGVSVSGYIAQQSIRLRIRDRDLVGPLITAFSES
                     VGNALAIDNISLQIDDVSPLLTQARRLAFEDANAKARQFAELASRELGPVLYVVDSPS
                     PGGGLHAGGAPKREMMMASASADMPVEMGENSIRCQVTVRWAWA"
     gene            3456..4079
                     /locus_tag="PKU75_09500"
     CDS             3456..4079
                     /locus_tag="PKU75_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81154.1"
                     /translation="MSWLDILGWAGSALLVVSVMQTRILRLRLLNLAATIALLVFNWG
                     LGIWSMVAMNGVLAALNVYYIAKLQRENTSASTAYEVIPVDHDEASLTHFLARNGRDI
                     ARFNPGFAGPQSGAAYLIQKGDTTCGVVLVRDIGDGVAQVDLDYVTAPYRDFSPGKFV
                     FRDSGLFQRKGFRRVLTRPGMVAPHYGKLGFVPDGDRWALELDTSTT"
     gene            complement(4112..4478)
                     /gene="ssrA"
                     /locus_tag="PKU75_09505"
     tmRNA           complement(4112..4478)
                     /gene="ssrA"
                     /locus_tag="PKU75_09505"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5090..5911)
                     /locus_tag="PKU75_09510"
     CDS             complement(5090..5911)
                     /locus_tag="PKU75_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPF81155.1"
                     /translation="MTYLQTLLRNAKFITASPDGSYGPVTAGAVQAVSEAYRVLRRDG
                     TTTTATWAVLEKINKPFDKYTCGNGSSNKVLLVFDDQPVSTTSYRALIDAARAGRYGI
                     GIAPNGEHVKSGLVDVKAARDRGLLVVDHTYHHYPLTTLSSEQVVWEITRSYVGSNYV
                     RPPYGDENSRIRAILAQYGKNDCLWNVDPQDWKRVGRPLPLEVPDHRITPSQVADYIV
                     GHAWGGSTVVVHMNHLGTDARQLARIDEGLRARGLMLCRNWGRASNPSMPNQYCL"
     gene            complement(6145..8118)
                     /locus_tag="PKU75_09515"
     CDS             complement(6145..8118)
                     /locus_tag="PKU75_09515"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPF81156.1"
                     /translation="MPWARRALAALVLAFAVLAGVAPAYATASGLERGFVAVGTGERI
                     TAYDNTMQLAEDGSVRVTETITHQFPSSGPERHGITRDIRVRAGYQNREDVYRYYRMS
                     DLTVSSPTGAPADVSESDFGAYIHVRIGSPDETVSGTQTYVISYTLAHVVNAIDDQRA
                     EFVHDVVGIANEQWYDQINATVEAPAAPLQVGCNYGAQGSNSICEATSGSPSTFAHTD
                     LKPGEAMTVAVSLPRSDFGDLEPELIEGSTSGEDTGASTVPPATSRLLGQLTAGLGVL
                     LPLLATAGMSALVWTRGRDEQYAGLTPGLSPGVGEEGRTVRRARPGTVAVQFNPPPGV
                     QPGMVGTVVDESADTVDVTATILDLAVRGYLTITETEKSFGRADWLLTLTTPPQTAHA
                     LSEYEEMLLDGLFSSGSPVRLSDLKNTFSSTLTAVKGSMYNEVVRRGWFRRSPQSQRN
                     SWSGLGTLLIFGGIASAFFLGGALPAGRPFSGVPLAGTTILAIGLVISGLVVKALGRR
                     MASRTADGSAVLAQSLGFRQYLVTAEANQIKFEEAQQIFSAYLPYAIVFGVAEKWAHT
                     FEQVAAAAAAQGIVISPPIWYVGPSWGTGGFFDSLTSGVDDFATQAAGTFISTPGSSG
                     TSVFDGGGGFGGGFGGGGGFSGGGGSGSSGGSW"
     gene            complement(8121..8594)
                     /gene="smpB"
                     /locus_tag="PKU75_09520"
     CDS             complement(8121..8594)
                     /gene="smpB"
                     /locus_tag="PKU75_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPF81157.1"
                     /translation="MAKETGRKVIASNRKARHDYLIEDTYEAGITLMGTEVKSLRMGR
                     ASLLDGFAIFYGDELWLEGVHIPEYVQGTWTNHTPRRRRKLLLHRTELDKLMAATRES
                     GRTIVPLSLYFKDGRAKVEIGLAKGKKAYDKRHALRERQDQRDMDRALNSRGRGE"
     gene            complement(8808..10193)
                     /locus_tag="PKU75_09525"
     CDS             complement(8808..10193)
                     /locus_tag="PKU75_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPF81158.1"
                     /translation="MCSRQAITAAIAGSALVAAAVAGGPATADLRDDKRKVEAKIEKL
                     HDDLDDTSTKLIEANALLEETQAKLPAARNALAAAQQAQADAQARNEQAKADLARAQA
                     DEQKASADLEATAAEMKDTQSRIANYAAQLYQEQGTGTLELALETSDLQQLTDRMVMA
                     DTLGDIQSDALAQLSTSNASLRATQDFLTATREKVADAQRATEATLAEARAATATAAQ
                     AEADLESLLDTREKAAAALDSEREAEKQRLAEYEDQSDEITTKLKEIEARQRREAAER
                     RKREEEERKRAEAAAAAAQNNSGGTNSGGSGGTSSSGGTGSSGGSGGSTNPPPSGGFL
                     STPVPGAWVSSEFGMRYHPILNYWRLHSGRDYAASCGTPVYAAAPGSIVSAGWGGGYG
                     NQVVIAHGLVDGDSLATTYNHLERIVATGGSVARGQLVGYVGTTGLSTGCHLHFEARV
                     SGVPKDPRNWL"
     gene            complement(10321..11235)
                     /gene="ftsX"
                     /locus_tag="PKU75_09530"
     CDS             complement(10321..11235)
                     /gene="ftsX"
                     /locus_tag="PKU75_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPF81159.1"
                     /translation="MRLRYLFGDVWVGLRRNMSMAISVILVTMISLFLLGLGLLAQRQ
                     ADVMKGYWYDRVQVSIFLCSNKSTQGNCLGHAVTEDQKANIRSQLDDLRPLVKEVFYE
                     SPQEAYDRFREQFKDTPLAGNIKVGDIPQSYRVQLSDPAKYEVVASTFENAPGVSMVQ
                     DQKKVLDKLFAILNKFTQYAVGLAVIMVICAVLLMATTIRQAAFSRRREIGIMRLVGA
                     SNMAIRLPFILETVIAALVGAGLALGLLTIGVRALVGDSGSQLAQALNQATIGLSDVW
                     RLAPILGGTAAALAIVTATITLWRYLRV"
     gene            complement(11235..12026)
                     /gene="ftsE"
                     /locus_tag="PKU75_09535"
     CDS             complement(11235..12026)
                     /gene="ftsE"
                     /locus_tag="PKU75_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPF81160.1"
                     /translation="MIRFENVTKSYPRSNRPALDDVSLEIERGQFVFVVGPSGSGKST
                     LLRLVIREEPTTSGSVLVAGHDLGNLPPRKVPRLRRQIGTVFQDFRLLPNKTVAQNVA
                     FALQVLGKPNRAIRMLVPETLELVGLEGKGRRMPHELSGGEQQRVAIARAVVNKPPIL
                     LADEPTGNLDPATSLDIVKLLASINTQHGTTVIMATHDNVIVDELQRRVIELDNGLVV
                     RDEEQGSYVPRSTEVSTDPAPSPDEDDLWANPDPDWTEDVVRGDD"
BASE COUNT         1881 a         4111 c         4306 g         2036 t
ORIGIN      
        1 tgatcgacac agtcctgggc gggtgtgtcg gtggcgacca ccttggcctg agccggcaag
       61 gcccccatac cgacggcgag cagtgcggcg ctcggcactg cgagcaagac gcgacgagac
      121 atgtgggttg gtgtcctctc tgaatttccc catacggccg gagccctggc cccgatccgg
      181 gaacacccta acgggttccc acctggatcg gggcacgacc ccaggccggt catcccgcag
      241 taccacccaa gcgtggaata cggcggcgcc acgcccttgc cggaggtacg tctggtcaga
      301 acactgtctc tccccggggg acgccagtgg ggccggtccg tatcggaccg gccccacccc
      361 gcggcatacg cgctagcgcg tcagagcatg cggtaggtgt tccacacgcg ctgcatccgc
      421 cacttctgcc cgttggtgaa gtggttcatg cacgggtcct cgctgtagtc catgaagttc
      481 ttcaccgggt cgttgccggg gcggttcttg caggtgtcgc ggccgaccgg gcagccgaac
      541 gcgggcgacg cctcgtacgg ggtgtcgaag acctggtcgc ccggggtctt gcagccgccc
      601 tggaaggtgt gccacaggcc catccagtga ccgacctcgt gggtgagggt gtcgccctcg
      661 ttgtagggcg cggcggtgcc gccgggcacc gactcgtcga ggatgacgac accgtcacgc
      721 gacgtgcctg cgtcctccca cgggaaggtg gcgtagccca gcaggccacc gctgaggttg
      781 gcgctgtaga tgttgaggac gttggtgccg ccgcggtgca gcttggcctt gatcgcgtcg
      841 tcgtattcgt aggactggcc catgttgttg taccaggcct tgttgttgac gtacgtgacg
      901 cccttgagcg cgaagtcgat gccgacgaat cgataggcgt cgtcgaggac cttgatcgac
      961 cggttgatct tggtcatgga cagcttgccc ttggcaccgt cgtggatgac gttgacgaag
     1021 acggggatgg tgacgtcgaa cggctcccgc tgtgcgccgt ccaggcgcac acccgcggcg
     1081 cgggcgtcgg cccggaactt cgcctccatc tcggcggcct tcgcgtcgct gacggagttc
     1141 cagtcgcggg cggttgcgcc cttggcgagg cgggcgccgg cgggcgcaca ctcggccgcg
     1201 gtggcgccgt cgccggcgac cgggcgggct tgggccggtg tggcgacgag accggccgcg
     1261 gcgagcgctg cgacgctggg gatggtcaat gcgatcttgc gattcattac ttccctctcc
     1321 tgatcttccc cgaagagccg gatgaacgcc ccccggacgg tgctcctccc tgaaacacct
     1381 gcctgcgaat ctagcggtgt ctcacatcgt ttggggggct gggaggttga tacatcggcc
     1441 cggacctgcg caaatacgct gcgcgtccta gtcctcgccg tcctgggggt cgcgcagccc
     1501 gtcgtagatc tccttgcaca gcggacagac gggaaacttc ttcgggtccc ggccggggac
     1561 ccagatcttg ccgcacaggg ccacgaccgg ttcgccggac agcgccgact cgaggatctt
     1621 ttccttgcgc acgtagtgac tgaagcgctc gtggtcgccc ggctcctgaa gttcggggac
     1681 gacctcggtc cgctcgaggg tggcggtgct cgtgctcggc cggctcgggg ccgccggcgc
     1741 gtccagtcct ggagtctcgc tcatggagaa agcgtaggcg accggtctca gttgagcccg
     1801 ggatcggcag ggtatgccgc gacgaacgcc agcgggccgg gctggcgcgc caggaccgca
     1861 cgccacaagt cggtgccggc ggcgaaggcg tcggcgggtt cgcgctcgac gacataccag
     1921 ctgcccgcca cgatctcccc ctcgagctgg ttggcgctcc agccggcgta accggcgaag
     1981 atgcgcacgg cgcagacctg ccccgagatc acctgcgggt cgctgtcgag atcgaccagg
     2041 ccgatgctgc cgaagagccg gcgcagccgg gaatcggccg gcggcaggtc gccgagcgac
     2101 accaaaccga gcgccgagtc catctggacc ggtccgccct ggaagaccat cgccggcaat
     2161 gtcgcgaccg caccccatcc gggcaggatc gcctccacct cagcctccag gggcttgttg
     2221 agcacgaccc cctgcgcgcc gtccgtgccg tgatggagca cgagcacgac gctgcgacag
     2281 aagatcccgt cctcgatctc cggcgtggca accagcagcc ggcccgtcag atcctccggg
     2341 atggtcatga gtcctattgt tgcgcgccgc ggccgttcgc ggggctcggc gccggtgccg
     2401 gaccggccgg tcagcgagct cggaatccgg cccccagcag gacggcgatg ccgatgccca
     2461 cgagatcggc acaacagtcc cagaccgagc ccgagcgggt cgcggtcaag cgcgcctgaa
     2521 ggatctcgct caggacggcc cacccggcca tggcgggcag ccacacggcc ggtttcagcc
     2581 ccgcgcgcaa cccgaggtag gccggcacgc cgaagaggac acagtgaatc accttgtcgt
     2641 cgtagggaaa cagcggctcc ccgccccgac tgggcatgaa gagcaagacg acgttcacca
     2701 ccaggctcgc gatcagggcc acccacacga ggaggcgcgg cagcggcata cgactcgctg
     2761 cgcttgcgcc gatgcgtgcc gtccccctgt tgtccatgac gggcactact gtgcaccaca
     2821 tggatgcctt cgtggaaatg accggtaccg gggtcgccag cgccccgccg gatgtggtcg
     2881 tcctcgacac cgccgtccgg accccgggcg ggtccgttgc cgaagccctc tcgcgggccg
     2941 ataccgcgat gaccgccatc gtgggcatcg cccgcgaacg cggcctcgcc ccggccgacc
     3001 tgcagaccac ctctgcatcg gtgtatccgc aatacgaccg cgagggcgtc tccgtcagcg
     3061 ggtacatcgc ccagcagtcg atacggctgc gcatccggga ccgcgacctc gtcgggccgc
     3121 tcatcacggc cttctccgag tccgtcggca acgccctcgc catcgacaac atcagcctgc
     3181 agatcgacga tgtgtccccg ctgctcaccc aggcccgccg gctggcgttc gaggacgcga
     3241 acgccaaggc gcgccagttc gccgagttgg ccagtcggga gctgggcccc gtcctgtatg
     3301 tcgtcgactc gccgagtccg ggaggcgggc tccacgcggg cggggccccg aaacgggaga
     3361 tgatgatggc ctccgccagt gccgacatgc cggtcgagat gggcgagaac tccatccggt
     3421 gccaggtcac cgtccgctgg gcctgggcat agccgttgtc ctggctcgac atcctcggat
     3481 gggccgggtc ggcgctcctc gtcgtctcgg tgatgcagac acgcatcctg cggctgcgcc
     3541 tgctcaacct ggccgcgacg atcgcgttgc tcgtcttcaa ctggggcctg ggcatctggt
     3601 cgatggtggc gatgaacggc gtcctcgcgg ccttgaacgt ctattacatc gccaagctgc
     3661 agcgcgagaa caccagtgcc agcacggcat acgaggtgat ccccgttgat cacgacgagg
     3721 cgagtctgac ccatttcctc gcgcgcaacg gccgtgacat cgcccggttc aacccggggt
     3781 tcgcggggcc gcagtccggt gcggcatacc tgatccagaa gggcgacacg acctgcgggg
     3841 tggtgctggt gcgcgacatc ggggacgggg tagcccaggt cgacctggac tatgtcaccg
     3901 cgccctatcg cgacttctcc cccggcaagt tcgtcttccg cgactcggga ttgttccagc
     3961 gcaagggttt tcgccgcgtg ctaacccggc cgggcatggt ggcgccgcat tacgggaagc
     4021 tggggttcgt gcccgacggc gaccggtggg cgctcgagtt ggacacgtcc acaacgtgat
     4081 gggtcgacca gcggctcggc gagccgagcg ctggtggagg tggcgggaat cgaacccgcg
     4141 tccactgacg gcgaaccagg acttctccgg gtgcagtgcg ctgagggagt ttctcggccc
     4201 cagggctcgc gcgcacacgt tccctgacag gcccagtcga gtaggagtcc cgcgccgccc
     4261 ctcgacatga cgacgcagca agatctctag ctgacgctgg tgactatgcc gagatcaggc
     4321 ataggccaac ggacttcggg ctcgctcagg cggcgagggc gaagtcggtg cgcttggagt
     4381 tggcacctat tggtttgcac ggggcgttca cgagataacc gtgcgtcctc gacccgcttc
     4441 ccctggaaga ccgaccaatg tcgaaaccga tcacccccga agggtgatcg acccatcacg
     4501 ctgtggattt gtcaagatcc ccaccattgt cggtggagta gttcagtatg acgcgtaacg
     4561 ctccgcagcc caagtcgatt cccgggagtt ctccgtatgc cgcgtgctcg cctccccagg
     4621 gctgtgctcg ccgccgttct ggtcgccgca gccgcggtgg gctgctcgac cgggggtgac
     4681 cggtccgcct cgacggggac cggggtcacc gcgacggcgc ccccgccgac ggtgccggcg
     4741 ggctgtcgtg acaacactgc cataccccgc gaacggatcc ccgcgaccct ggccccgctg
     4801 ccgggcccga agaccgcccg gtcgctgctc cagaagctga ccgtcgccga tcgctcacgc
     4861 ggggatgact tctgccgcaa cgctttcggg cctgggtggc cggaactcga cggcaacggg
     4921 tgcagtgccc gccaggacac cctgcgccgg caaggcactc agctgcgcct gcaggtgatc
     4981 cgcagccatg ggaccggttg cccctgtgca ggtgaagtac ggctacgagc tcacggtcac
     5041 cgaggacgag cgggacgccc tcgcggacat gctcgggacc tgctgagact cacaggcagt
     5101 actggttggg catcgagggg ttgctcgccc ggccccagtt ccggcacagc atgagacccc
     5161 gggcgcgcaa cccctcgtcg atccgggcga gctgtctcgc gtcggtgccg agatggttca
     5221 tgtgcacgac gacggtcgac ccgccccagg cgtggccgac gatgtagtcg gccacctgtg
     5281 acggggtgat gcgatggtcg ggcacttcga gcggcagggg ccggcccacg cgcttccagt
     5341 cctgcgggtc gacattccac aggcagtcgt tcttgccgta ttgcgcgagg atcgcccgga
     5401 tccggctgtt ctcgtcgcca tacggggggc gcacgtagtt gctgccgaca taggagcgcg
     5461 tgatctccca gaccacctgc tccgagctca gcgtcgtgag cgggtagtgg tgataggtgt
     5521 ggtcgacgac gagcagcccg cgatcccgcg cggccttgac gtcgacgagg ccggacttga
     5581 cgtgctcacc gttgggggcg atgccgatgc cgtaacgccc ggcccgggcg gcatcgatca
     5641 gggcccgata actcgtggtg gagacgggct ggtcgtcgaa gacgagcagc accttgttgc
     5701 tcgagccgtt gccgcaggtg tacttgtcga agggcttgtt gatcttctcc aagacggccc
     5761 acgtggcggt cgtcgtggtg ccatcgcggc gcagcacccg ataggcctcg gacaccgcct
     5821 gcaccgcacc ggcggtgacg ggcccgtagc tgccatcggg gctggccgtg atgaatttgg
     5881 cgttccggag gagggtttgc agataggtca cgcagctgcc cgtgtcgccg accttcaaag
     5941 agggccgtga cgacaccggt gtggaagagc tgcacgaaac ggccgacgca tgggcgccgc
     6001 ccaccgatgt cgtgagcagg ggggccgcga cgactgtcgc tgccagcgtg aaccgtgcga
     6061 atgtccgccc catgcgcggg aacttagcag ccatacctcg ttgacgcccc aggagggggt
     6121 gcggttgtca ccgagacgtc actgttacca ggagccgccg gaggatccgg agccaccgcc
     6181 accggagaag cccccgccgc cgccgaaacc gccgccgaag ccaccgccgc catcgaagac
     6241 ggaggtgcct gaggagcccg gcgtggagat gaaggtgcct gccgcctggg tggcgaagtc
     6301 gtcgacgccc gaggtcagcg agtcgaagaa gccgcccgtg ccccagctcg ggccgacgta
     6361 ccagatgggc ggggagatca cgatcccctg cgcggccgcg gcggccgcca cctgctcgaa
     6421 ggtgtgggcc cacttttcgg cgaccccgaa gacgatcgca tagggcagat aggccgagaa
     6481 gatctgctgg gcctcttcga acttgatctg attcgcctcg gccgtcacga ggtattggcg
     6541 gaacccgagc gactgcgcga gcacggcact gccgtcggcg gtgcgcgaag ccatgcgacg
     6601 gccgagggcc ttgacgacca ggccgctgat gacgaggccg atggccagga tggtcgtgcc
     6661 cgccagcgga acgcccgaga acggccggcc ggccggcagg gcaccgccga ggaagaacgc
     6721 agaggcgatg ccgccgaaga tgaggagggt gcccaggccc gaccaggagt tgcgctggct
     6781 ctgcggcgac cggcggaacc agccccggcg cacgacctcg ttgtacatcg agcccttcac
     6841 cgccgtcagg gtcgaggaga aggtgttctt cagatccgac agccgcaccg gcgagccgct
     6901 cgagaacaga ccgtcgagga gcatctcttc gtattcgctg agcgcatgtg ccgtctgcgg
     6961 gggcgtggtc aaggtcagca gccagtcggc gcggccgaag gacttctcgg tctcggtgat
     7021 cgtcaggtag ccgcgaaccg cgaggtcgag aatggtggcg gtgacgtcga cggtgtcggc
     7081 gctctcgtcg accacggtgc cgaccatgcc cggctgcact ccaggcggcg gattgaactg
     7141 caccgcaacg gttccgggac gcgcgcgccg cacggtccgg ccctcctcgc cgaccccggg
     7201 agacagaccc ggggtcaggc cggcatactg ctcgtcgcgc ccccgcgtcc acaccagtgc
     7261 actcatgccc gccgtggcga gcaggggcag caggacgccg agcccggcgg tgagctggcc
     7321 caacaggcgc gacgtggccg gcggcacggt ggaggcgccc gtgtcctccc cggacgtcga
     7381 gccctcgatg agttcgggct cgaggtcgcc gaagtccgag cgcgggagcg agacggcgac
     7441 ggtcatcgcc tcccccggct tcaggtcggt gtgggcgaag gtcgaggggc tgccggatgt
     7501 tgcctcacaa atggagttcg agccctgcgc gccgtagttg caacccactt ggagcggcgc
     7561 ggccggcgcc tcgacggtgg cgttgatctg gtcataccac tgctcgttgg cgatgccgac
     7621 gacgtcgtgg acgaactcgg cccgctggtc gtcgatcgcg ttgacgacgt gagcgagcgt
     7681 gtaggagatg acgtaggtct gtgtgcccga cacggtctcg tcggggctgc cgatccgcac
     7741 gtggatgtag gcgccgaagt cgctttcgct gacgtcagcg ggagcgccgg tcggggagga
     7801 gacggtcagg tcgctcatcc ggtagtagcg gtagacgtcc tcgcggttct ggtaccccgc
     7861 acgcacccgg atgtcacggg tgatgccgtg ccgctccggg ccgctgctgg ggaactggtg
     7921 ggtgatcgtc tcggtcaccc gcaccgaacc gtcctcggcg agttgcatcg tgttgtcgta
     7981 tgcggtgatc cgctccccgg tgccgacggc gacgaagccg cgctccagcc cggaggccgt
     8041 cgcgtatgcc ggggccacgc ccgcgaggac ggcgaacgcc agcacgagcg cggcgagcgc
     8101 gcggcgagcc cacggcatac tcactctccc cgcccgcggg agttgagcgc gcggtccatg
     8161 tcgcgctggt cctgccgctc gcgcaacgcg tgcctcttgt cgtaggcctt cttgcccttg
     8221 gccagcccga tctccacctt ggcgcgcccg tccttgaaat agagggatag ggggacgatg
     8281 gtgcggccgc tctcgcgcgt ggccgccatc agcttgtcga gttcggtgcg gtgcagcagc
     8341 agcttgcggc gccggcgggg ggtgtggttg gtccaggtgc cctggacgta ttcggggatg
     8401 tgcacaccct cgagccacag ttcgtcgccg tagaagatgg caaagccgtc gagcagggag
     8461 gcccggccca tgcgcagcga cttcacctcg gtgcccatga gggtgatgcc cgcctcgtag
     8521 gtgtcctcga tgaggtaatc gtggcgcgcc ttgcgattgc tcgcgatcac cttgcgcccc
     8581 gtctccttgg ccaccgtcac ccgtccttcg cctcgcggcg gtgcccggcg caccgcccgc
     8641 tcaggttacc cgccaggttc gggcgaagcc cgccaatcgg cccgcaggcg tccgcgacgg
     8701 tgtcttggtg acattcacaa tacgaagtgg gcgattcgac cggtcaacca cgcctgccgg
     8761 ggctctcctc gtatttcgac tgtccgcctg ccttgcactt tgggcggcta cagccagttg
     8821 cgggggtcct tggggacgcc gctcacgcgg gcctcgaagt gcaggtggca gccggtggac
     8881 aggcccgtcg tgccgacata gccgaccagt tggccgcggg cgaccgagcc gccggtggcg
     8941 acgatgcgct cgaggtggtt gtacgtggtc gccagcgagt cgccgtccac caggccgtgg
     9001 gcgatgacga cctggttgcc gtaaccgccg ccccagcccg cggagacgat gctgccgggg
     9061 gcggcggcat acacgggggt gccacaggag gcggcgtagt cgcggccgga gtgcaggcgc
     9121 cagtaattga ggatcgggtg gtagcgcatc ccgaactcgg aggaaaccca ggcgccgggg
     9181 accggtgtgg acaggaagcc gccggatggc ggcgggttgg tggacccacc ggaaccaccg
     9241 gagcttccgg tcccgccgga acttgacgta cctccggagc cgccggagtt cgtgccaccg
     9301 gaattgttct gggcggcggc agcggcggcc tcggcccgct tgcgctcctc ttcctcacgc
     9361 ttcctgcgtt cggccgcctc gcgtcgctgg cgtgcctcga tctccttcag cttggtggtg
     9421 atctcatccg actggtcctc atattccgcg agccgctgct tttcggcctc ccgctcggag
     9481 tccaaggcgg cagccgcctt ctcgcgggtg tcgagcaggg actccagatc ggcctcggcc
     9541 tgagcggcgg tcgcggtggc ggctctcgcc tcggcgagcg tggcctcggt ggcgcgttgc
     9601 gcgtcggcga ccttctcgcg cgtggcggtc aggaaatcct gcgtggcccg cagcgaggca
     9661 ttcgaggtcg acagctgggc cagggcgtcg ctctggatgt cgccgagcgt gtcggccatg
     9721 accatgcggt cggtcagttg ctgcaggtcc gaggtttcga gcgcgagttc gagagtgccg
     9781 gtgccctgct cctggtagag ctgagcggca tagttcgcga tccgcgactg ggtgtccttc
     9841 atctcggcag cggtcgcctc gaggtccgcg ctcgccttct gttcgtcggc ttgcgcgcgg
     9901 gcgaggtcgg ccttggcctg ctcgttgcgg gcctgggcgt cggcctgggc ctgctgggcc
     9961 gccgccagcg cgttgcgggc ggcgggcagc ttggcctggg tctcctcgag gagggcgttg
    10021 gcctcgatca gcttcgtgga cgtgtcgtcg agatcgtcgt ggagcttctc gatcttggct
    10081 tcgaccttgc gcttgtcatc gcgcagatcg gcggtggccg gaccaccggc aacggcggca
    10141 gccacgaggg cactgcccgc aatggcggca gtgatggcct ggcgcgaaca cacgcgccca
    10201 ttcggtccca aacgcatcat cggccccaag gttagccagc cgggctgtgg aaatccggac
    10261 caccagcggc cgggcgtgtc gcacgatacc ggcggcgcgc gctccgggga agggctgggg
    10321 tcaaacgcgc aggtagcgcc agagcgtgat ggtcgcggtg acgatcgcca gggcggcggc
    10381 ggtgccgccg aggatgggag ccagccgcca cacgtcggac aggccgatcg tggcctggtt
    10441 cagcgcctgg gcgagttggc tgccgctgtc gccgaccagg gcccgcacgc cgatcgtgag
    10501 cagtcccagg gcgagcccgg cgccgacgag ggccgcgatg acggtctcga ggatgaacgg
    10561 caaccggatc gccatattgg aggcgccgac gaggcgcatg atgccgatct cgcgccggcg
    10621 gctgaacgcc gcctgccgga tcgtcgtggc catcagcagc acggcgcaga tgaccatgat
    10681 cacggccagg cccacggcat actgagtgaa cttgttgagg atggcgaaga gtttgtccag
    10741 caccttcttc tggtcctgga ccatcgagac accgggggcg ttctcgaagg tgctggcgac
    10801 gacttcgtat ttcgccgggt cggacagctg cacccgatag ctctgcggga tgtcaccgac
    10861 cttgatattg ccggcgagcg gagtgtcctt gaactgctcg cggaagcggt cgtaagcctc
    10921 ctgcggcgac tcgtagaaga cctccttcac cagcggtcgc aggtcgtcca gctggctgcg
    10981 gatgttggcc ttctgatcct cggtcacggc gtgtccgaga cagttgccct gggtcgactt
    11041 gttggagcac aagaagatcg agacctggac ccggtcatac caatacccct tcatcacatc
    11101 ggcctggcgc tgcgcgagca ggccgaggcc gagcaggaag agggagatca tcgtgacgag
    11161 gatgaccgag atcgccatcg acatattgcg gcgcaggccg acccagacat cgccgaacag
    11221 gtagcgcagc cgcatcagtc gtctcctcgc acgacgtcct cggtccaatc cgggtcggga
    11281 ttggcccaca ggtcgtcctc atcgggcgac ggcgccggat cggtcgagac ctccgtggac
    11341 cgggggacat agctgccttg ctcttcgtcg cgcacgacga ggccgttgtc gagttcgatg
    11401 acgcggcgct gcagttcgtc gacgatgacg ttgtcgtgcg tcgccatgat caccgtggtg
    11461 ccgtgctggg tgttgatgga ggccagcagc ttgacgatgt ccagactcgt cgcggggtcg
    11521 aggttgccgg tcggctcgtc ggcgagcagg atcggcggct tgttgaccac ggcccgggcg
    11581 atggcgacgc gctgctgctc gccaccggag agttcgtgcg gcatccggcg gcccttgccc
    11641 tccagcccga cgagttcgag ggtctcgggg acgagcatcc ggatcgcgcg gttgggcttg
    11701 cccagcacct gcaacgcgaa ggcgacgttc tgcgcgaccg tcttgttggg cagcaggcgg
    11761 aagtcctgga agacggtgcc gatctggcgc cgcagccgcg gcaccttacg cgggggcagg
    11821 tttccgaggt cgtgccccgc aacgagcacc gaccccgatg tcgtcggttc ctcgcggatg
    11881 accagccgca agagcgtcga cttgcccgag ccggacggcc cgacgacgaa cacgaactgg
    11941 ccgcgctcga tctccaggga aacgtcatcg agggccgggc ggttcgagcg cggatagctt
    12001 ttcgtgacgt tttcgaagcg gatcatgaag ggcgggtcgg cctaacagtt ggcgttacgg
    12061 ggagcgctga gacggaaggc ataggtgctt cagtctaagc agggcgcctg tgctgtcacc
    12121 cacttcctcg gcccagatgc gtgtcggcgt ggtgctcgct gcaaagcgat acccaccaat
    12181 cactactgtc ccaccacgat cttcatcggc tacgttgtcc cccggcaccc atccgccgaa
    12241 ggtctccccc gggctttcgg cgccgcccaa ggagtcagcc gcatgccccc caccacgccg
    12301 ttgcgtcccg ccctcgtcat cgccggtgtc accc
//
LOCUS       DAOPJG010000093        12322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5404_length_12322_cov_7.711013, whole genome shotgun sequence.
ACCESSION   DAOPJG010000093 DAOPJG010000000
VERSION     DAOPJG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5404_length_12322_cov_7.711013"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKU75_09540"
     CDS             <1..192
                     /locus_tag="PKU75_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPF81161.1"
                     /translation="NDGADLLNSVIAARAAAHGFTFVDVTKRFDGHGINAPEPWLLGL
                     GPSCALRTRYSAVRRWCRP"
     gene            complement(170..511)
                     /locus_tag="PKU75_09545"
     CDS             complement(170..511)
                     /locus_tag="PKU75_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPF81162.1"
                     /translation="MPKLTEVSESSAHVGLTEALPVVAESTTSRVLVNNDLLRVVQFT
                     FDEGEMLTEHASPRAVVVQLVSGTMRFTVDGVEHTMGEGDVIYLAPGARHALVADTAC
                     RMTLVMVDTTA"
     gene            complement(531..1691)
                     /locus_tag="PKU75_09550"
     CDS             complement(531..1691)
                     /locus_tag="PKU75_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF81163.1"
                     /translation="MNADFDTYRLSEDHEAIRHAVREIAEAKIAPYAAAVDEEARYPQ
                     EAHDALVASDFFAPHVAEEFGGAGADALATCIVIEEVARVCVSSSLIPAVNKLGSMPV
                     IIGASEEVKKKYLTPLAAGTGGFSYGLSEREAGSDTASMRCKATDNGDGTFTLNGQKA
                     WITNAGVSEYYTVMAVTDPDGPRGGNVTAFVVEKSDPGFTFGEKEKKLGIKGSPTREL
                     IFTGCVIPEDRMVGGRGEGLKIALRTLDHTRVTIGAQAVGVAQGALEQATAYIKERKQ
                     FGKAIADFQGVQFMLAEMAMQLEAARQLVYVAAAKSERNASDLTFFGAAAKCFASDVA
                     MQVTTDAVQLLGGAGYTKDFPLERMMRDAKITQIYEGTNQIQRLVMARQLLR"
     gene            1844..3049
                     /locus_tag="PKU75_09555"
     CDS             1844..3049
                     /locus_tag="PKU75_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81164.1"
                     /translation="MFARFRRYPSVVVAATVLPALVAAPGVAAEPGSPVHAPAGFAAG
                     WVNPRSSNWFDVLLFATGKVAGRPGNVHAIRVSWDEGIMVDITDYACPTGIVGPAGCA
                     KSGSETLYSSPGAATMTRVGRVGVDIAGTVSGDSGATYPVRLSLRPLGRIGYTIHSPY
                     GERLMQRWDALRGTAAGRVGPWGLSQEGVTATAVSLTGATSYEWSGPGAPRPSAERDW
                     VSTSDDRQGSVSWLRLGTVLGRPGNAHSGWVDTQVAWDAAADPWARVDAAEWNDWSCP
                     AGVQPPKFATPNHVCTLLRNDQTWYAETGGGRTGREGLRVAGSVPVRVLTASGIRNTS
                     TAVDVTLWPGGEHLRAPVWFGSGCKVVDTYANAAASTGLLGWVGLDSPRAKVTGGMLF
                     VEKSFCTTG"
     gene            complement(3068..4138)
                     /locus_tag="PKU75_09560"
     CDS             complement(3068..4138)
                     /locus_tag="PKU75_09560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPF81165.1"
                     /translation="MRFGIFVPQGWRMDLAGIDPAQHWGVMAGLARRADANPEWESIW
                     VYDHFHTVPMPSQEATHEAWTMMSAFAAVTGRVRLGQMCTCMSYRNPAYLAKVAATVD
                     IVSEGRLEMGIGAGWYEHEWRAYGYGFPRAGERLAALREGVEIFRQAWTTGSATLDGE
                     HYQVDGALCYPRPLQGTSAPGAPRNGIPMWVAGGGEKVTLRIAAEYADYTNFDGSPEG
                     FATKSALLQQHCADVGRDFGEIVRSANYNVFIGETEKEVEDRLAWFADHMRQAGVPES
                     KIDEQLPGFRNGPAVGTPEQIVERLSALQDMGMTYAITYFAESAYDLSGVELFEREVM
                     PALLDEPKADAHEHRWHLPGRR"
     gene            4208..5392
                     /locus_tag="PKU75_09565"
     CDS             4208..5392
                     /locus_tag="PKU75_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPF81166.1"
                     /translation="MSYSAPATIAVVGTSYVGLSLAMLLARHNTVRAHDIDEHKIAML
                     RAGSSPISDPDIERFLAEEDLDITYTTSPEEAYAGAEYVIIATPTNYDEVTNYFDTRS
                     VESVIKDVAHTNPDAVMIVKSTIPVGFTLGIRERLDTDNVIFSPEFLREGRALHDNLH
                     PSRIVVGELSDRAQRFADLLRDASEEPDVPVLLTGSTEAEAIKLFANTYLAMRVAYFN
                     ELDTFASTHGLNTRQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYQDVPQ
                     TLISAIVDANTTRKDFVAMDIVARQPKVVGIYRLIMKAGSDNFRSSSVQGIMKRIKAR
                     GIEVIVYEPEFAHGSWYGSEVVDSLEELKARADLIVANRQTPELADVADKVYTRDLFG
                     SD"
     gene            complement(5401..5844)
                     /locus_tag="PKU75_09570"
     CDS             complement(5401..5844)
                     /locus_tag="PKU75_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPF81167.1"
                     /translation="MTHQNDPDLVRNLLVDPGTWVIVGLSTNRQRDAWHISRYLKMEQ
                     GKAIIPVHPKAETVHGEQGYASISDIPDGTDVKVVDCFVNSEHVGAVVDDAIANKERL
                     DIDAIWMQLGVVDPDAAARARKAGIDVVMDTCPKIEYPKLRPASS"
     gene            complement(5924..6430)
                     /gene="purE"
                     /locus_tag="PKU75_09575"
     CDS             complement(5924..6430)
                     /gene="purE"
                     /locus_tag="PKU75_09575"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPF81168.1"
                     /translation="MNPVVGVVMGSDSDWPVLEQAAIALQEFDIAYEADVVSAHRMPE
                     DMIAYGRGAAARGLRVIIAGAGGAAHLPGMLASVTPLPVIGVPVPLKYLDGMDSLLSI
                     VQMPAGVPVATVSIGGARNAGLLAARILATGTDDEAARLRAAMVTFQESLRQTAYDKG
                     AALREQLS"
     gene            complement(6427..7593)
                     /locus_tag="PKU75_09580"
     CDS             complement(6427..7593)
                     /locus_tag="PKU75_09580"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HPF81169.1"
                     /translation="MNASAPTPLRAPGGFPVVGIIGGGQLARMCAGPAAELGVTLSVL
                     AEEGDGSAAQVVRSAPVGDYTDPATVLDFARHCDVVTFDHEHVPAPVLSALAQAGVPL
                     HPSPEALVFAQDKLAMRTRLTALGIPCPRWAAVATAAEAAAFGDSVGWPVIAKVPTGG
                     YDGKGVLVASAPEEVAEWLARGPVLLEELVAFRRELAVLIARSPSGQAAAWPVVETVQ
                     ENGICVEVHAPAKDLSPDLAADATAAGLRIAGELGVTGVLAVEIFETADGFVVNELAM
                     RPHNSGHWSMDGAITGQFEQHLRAVLDLPLGDPRPRCGVTVMANVLGGQYDSLYPAYK
                     HVMARDPGLRVHMYGKGVRPGRKIGHVNVVSESYADAHERARHAADYLQGVVTE"
     gene            7666..8184
                     /locus_tag="PKU75_09585"
     CDS             7666..8184
                     /locus_tag="PKU75_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPF81170.1"
                     /translation="MTSPAHAFVDWVRGTINVLWREVVKFGIIGALAFVVDVGTMNLL
                     RNTVMEDKPTTAKVVSASVATVFSWIGNRQWTFRARRNRPAHHEFALFAVTNIVALVI
                     GAGAIAFSHYGLGLKTLFADNIANVVGIGLGTLFRFWAYRKFVFANELDPASERNSPN
                     TAPSARNSSSLP"
     gene            complement(8085..9125)
                     /locus_tag="PKU75_09590"
     CDS             complement(8085..9125)
                     /locus_tag="PKU75_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPF81171.1"
                     /translation="MNRLFVRATMSVVAAVLATMAALVMSLLVIAGFRAPGDRVMDRV
                     PSTTFVFIVFGICAALAAVASILGLVVARRQAARVTAPLSALAAQATRLGSGESNMAP
                     LATGVSEVDAVSAVIAASAGDMARSLAAERDFAADASHQLRTPLAALMLRLEEIADTD
                     DPDVVKEEAGVAIAQVARLTDVVDDLMNRAKRRPPEAAVVSVDSVLAALQREWQPAFA
                     QTRRSMRVHGERDLQVFIARNALSQILSTLIENSLAHGSGTVSVAARHSGPTVVVEVS
                     DEGPGVDPGIARHIFERSVSSSGSGLGLALARDLAEKNYGRLELLRAEGAVFGLFLSD
                     AGSSSLAKTNLR"
     gene            complement(9129..9794)
                     /locus_tag="PKU75_09595"
     CDS             complement(9129..9794)
                     /locus_tag="PKU75_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF81172.1"
                     /translation="MTRVLLAEDDEAIAEPLARALRREGYEVEVRADGAEALEVALRG
                     VDLVVLDLGLPTMDGLDVCRRLRAAGSTTPVLILTARSDEVDTVVGLDAGADDYVKKP
                     FRLAELLARTRAMLRRGNADPENVPALVIDEESRRVFLRGEELSLTGKEFALLRVLHR
                     DRGKVVSREQLMHEVWDTEWYGSTKTLDVHISVLRRKLGDNDEPRLISTVRGVGFRLD
                     VDE"
     gene            complement(9791..10846)
                     /locus_tag="PKU75_09600"
     CDS             complement(9791..10846)
                     /locus_tag="PKU75_09600"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPF81173.1"
                     /translation="MRQDDLAVQEFLDHIQERLLGKPVATGRDEVSQRADLALSSVTA
                     FWHALGFPAEHSDSGVFTEADLVALHRIVSLIDDGGVDEQLALGMTRAVARTADRLSV
                     WQTQLVAESVAPDVSPDGLGADPQTAAEVARRLTAAADAFEPLLVYAWRRHLYAALTR
                     MVSDADPAQASSGPVRLVGFADLVNFTSVVRRATERELAVVVQRFERLASDIVTAHGG
                     RVIKTVGDEILFVNQDPVAGTAIALDLVDTMAEEEVLPQVRVGLAYGPVLSRLGDVFG
                     TTVNRASRLTAVAGEGRVLADEALAARLAQQSGFRLRTQRRRLLRGIGTHTPREVTRS
                     HGGRRSEGGGPLHTMGT"
     gene            complement(10866..11642)
                     /locus_tag="PKU75_09605"
     CDS             complement(10866..11642)
                     /locus_tag="PKU75_09605"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPF81174.1"
                     /translation="MRLSLDLPRIETALRESSVTQMLCHDQIDSTNTAALALDAPGAL
                     VLAEHQVGGKGRLGREWTETPRAGLAMSLRVPVPPHAVGWVPLLTGLALRTAVARETA
                     YRADLKWPNDLLAASGGKLAGILCEMTPDAIVIGAGLNVDHTREELPVPTATSLALEL
                     GRPIGAIDRSALVIAYVQELTTLLAALHTGGGELRAAQEAYRAACSTIGARITVQTQG
                     APVAAEATGVDDDGRLLVRDPAGATRAIAAGDVHHVRVAE"
     gene            11667..>12322
                     /locus_tag="PKU75_09610"
     CDS             11667..>12322
                     /locus_tag="PKU75_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPF81175.1"
                     /translation="MTADAAAQDVDIHTTAGKLADLKRRVAEVAHAGGPVAVEKQHAK
                     GKMTARERILTLLDEGTFIEMDKYARHRSNAFGQEKSRPYGDGVVTGYGLVDGRTVAV
                     FAQDFTVFGGSLGEVFGEKIVKVMDFAMKIGCPVVGLNDSGGARIQEGVVSLGLYGEI
                     FLRNVHASGVIPQISLIMGPCAGGAVYSPAITDFTIMVDKTSHMFITGPDVIKTVTGE
                     "
BASE COUNT         1894 a         4369 c         4180 g         1879 t
ORIGIN      
        1 aacgacgggg cggacctgct caactccgtc atcgccgccc gcgccgcggc ccacggcttc
       61 accttcgtcg acgtgaccaa gcgcttcgac ggccatggca tcaatgcacc cgagccgtgg
      121 ctgctcggac tgggcccctc ctgcgcgctt cggacgcgat actcggccgt caggcggtgg
      181 tgtcgaccat gacgagggtc atgcggcagg cagtgtcggc caccagcgcg tgacgggcgc
      241 ccggggcgag gtagatgaca tccccctcgc ccatcgtgtg ctcgaccccg tcgacggtga
      301 atcgcattgt gccgctgacc aattggacca cgacggcccg aggcgaggcg tgctcggtga
      361 gcatctcccc ttcgtcgaag gtgaactgca cgacacgcag caggtcgttg ttgaccagga
      421 cgcgtgaggt cgtcgactcg gcgacgaccg gcagcgcctc ggtcagaccg acatgcgcgg
      481 acgactcgga gacttcggtc agcttgggca tgttcgtgag cttagggccc tcagcgcagc
      541 agctggcggg ccatcaccag gcgctggatc tggttggtgc cctcgtagat ctgggtgatc
      601 ttggcgtcgc gcatcatccg ctccagcggg aagtccttgg tgtagcccgc accgccgagc
      661 aactggaccg cgtcggtggt cacctgcatg gcgacgtcgg aggcgaaaca cttcgcggcg
      721 gcgccgaaga aggtcaggtc gctcgcattg cgctcggact tggccgccgc gacatagacc
      781 agctggcggg ccgcttcgag ctgcatcgcc atctcggcga gcatgaactg cacaccctgg
      841 aagtcggcga tcgccttgcc gaactgcttg cgctccttga tatatgccgt ggcctgctcg
      901 agcgctccct gcgcgacccc gacggcctgg gccccgatcg tcacccgcgt gtggtcgagg
      961 gtgcgcaggg cgatcttcag cccctcaccg cggccaccga ccatgcggtc ctcggggatg
     1021 acgcaacccg tgaagatcag ctcccgcgtg ggcgagccct tgatcccgag cttcttctcc
     1081 ttctcgccga aggtgaatcc gggatcggac ttctcgacga cgaaggccgt gacattgccg
     1141 ccgcgcgggc cgtccggatc ggtgaccgcc atgaccgtgt agtactcgga gacaccggca
     1201 ttcgtgatcc acgccttctg cccgttgagg gtgaacgtgc cgtcgccgtt gtcggtcgcc
     1261 ttgcaccgca tcgacgccgt gtccgacccg gcttcgcgct cggacaggcc gtacgagaag
     1321 ccgcccgtgc cggccgcgag cggggtgagg tacttcttct tgacctcctc gctcgcgccg
     1381 atgatcaccg gcatgctgcc gagcttgttg acggccggga tcagcgagga ggagacacac
     1441 acgcgggcga cttcctcgat gacgatgcac gtcgcgagcg catcggcgcc cgcgccgccg
     1501 aactcctcgg ccacgtgcgg ggcgaagaag tcgctcgcca cgagcgcgtc gtgcgcctcc
     1561 tgcggatagc gcgcttcctc atcgaccgcg gccgcgtagg gcgcgatctt ggcctcggcg
     1621 atctcgcgga ccgcgtggcg gatggcctcg tggtcctcgg acaggcggta ggtgtcgaaa
     1681 tcggcgttca cgcatccaca ctacggccgc tgttccggcc accccaagga cccttggcgt
     1741 gactcagatg actcccgggc atgggggtat gccgcgccgt cgcccgcacg agtagtctga
     1801 gaccagaggc cgtcgacgcc gacagccggg agtcaggtgc gccatgttcg ctcgtttccg
     1861 ccggtatccg tccgtggtcg tcgccgccac ggtccttccc gccctcgtcg ccgccccggg
     1921 tgtggccgcc gagcccggct cacccgtcca cgcacccgcc ggctttgccg ccggctgggt
     1981 gaatccccgg tcgagcaact ggtttgacgt gctgctcttc gccaccggaa aggtcgccgg
     2041 ccgcccgggc aatgtccacg cgatacgtgt ctcctgggac gaggggatca tggtcgacat
     2101 caccgactat gcctgtccaa caggcatagt cgggcctgcg ggttgcgcga agtccgggtc
     2161 ggagacgctg tattcgtcgc cgggagcggc gacgatgact cgggtcgggc gggtgggcgt
     2221 cgatatcgcg gggacggtga gcggcgactc cggcgccacg tatcccgtgc gactctccct
     2281 gcgaccgctg ggccggatcg gctacaccat ccacagcccc tacggcgagc ggctcatgca
     2341 gcgctgggat gctttgcggg gcaccgcagc cggccgtgtc ggcccttggg gactgtctca
     2401 ggagggagtc acagcgaccg cggtgagcct gaccggcgcc acgtcgtatg aatggtccgg
     2461 ccccggagca ccacgcccct cggcggagcg cgactgggtg agcacgagcg acgaccggca
     2521 ggggtcggtg tcgtggctgc gcctcggcac cgtcctgggc cgccccggca acgcgcacag
     2581 cgggtgggtc gacacccagg tggcctggga cgcggccgcg gatccgtggg cccgcgtcga
     2641 cgctgcggag tggaacgact ggtcgtgccc agcgggcgtg caaccgccca agttcgcgac
     2701 accgaaccac gtgtgcaccc tcctccgcaa cgaccagacg tggtatgccg agaccggcgg
     2761 cgggaggacc gggcgcgaag gcctgcgggt ggctgggagc gtgccggtgc gggtgctcac
     2821 cgcctccggc atccgcaaca cctccaccgc agtggacgtc accctctggc ccggtggtga
     2881 gcacctgcgc gcccccgtct ggttcgggtc ggggtgcaag gtggtcgaca cctacgccaa
     2941 tgccgcggcc agcaccgggc tcctgggctg ggtcgggctg gacagccccc gcgccaaggt
     3001 cacgggcgga atgctgttcg tggagaagag cttctgcacg accggctgag ccggtcacca
     3061 cggagggtca gcgtcgtccc ggcaggtgcc accggtgctc gtgcgcgtcg gccttgggct
     3121 catccagcag cgcgggcatc acttcgcgct cgaagagttc gacaccggac aggtcgtagg
     3181 ccgactccgc gaagtaggtg atcgcgtagg tcatgcccat gtcctgcaga gcggagagcc
     3241 gttcgacgat ctgctcgggt gtgccgacgg ccgggccgtt gcggaagccg ggcagttgct
     3301 cgtcgatctt cgactccggc acgcccgcct ggcgcatgtg gtcggcgaac caggcaagcc
     3361 ggtcctcgac ctccttctcg gtctcgccga tgaagacgtt gtagttggcg ctgcgcacga
     3421 tctcgccgaa gtcgcgcccg acgtcggcgc aatgctgctg caggagagcg gatttggtcg
     3481 cgaacccctc gggcgagccg tcgaagttgg tgtagtcggc atactccgcc gcgatccgca
     3541 gcgtcacctt ctcgccacca ccggcgaccc acatgggtat gccgttgcgc ggcgccccgg
     3601 gcgccgacgt gccttgcagg ggacggggat agcacagcgc cccgtcgacc tgatagtgct
     3661 cgccgtcgag cgtggcgctc ccggtggtcc aggcctgccg gaagatctcc acgccctcgc
     3721 gcagcgcagc gagccgctcc cccgcccggg ggaagccgta gccataggcc cgccactcgt
     3781 gctcatacca gccggcgccg atgcccatct cgagccggcc ctcggagact atgtcgacgg
     3841 tcgcggcgac cttcgcgaga taggccgggt tgcgatagct catgcacgtg cacatctgcc
     3901 ccagccggac gcggccggtc accgcggcga aggcggacat catggtccac gcctcgtggg
     3961 tcgcctcctg gctcggcatg ggcaccgtgt ggaagtggtc gtagacccag atcgactccc
     4021 actcggggtt ggcgtctgcg cgccgcgcga gcccggccat caccccccag tgctgtgccg
     4081 ggtcgatgcc ggccagatcc atccgccagc cctgagggac aaagatgccg aaccgcatac
     4141 ccccaaccta tcccgaccgc ggcgagccga tcggcccacg ccgcccgccc ccgtagaatt
     4201 gccgatcgtg tcctacagcg cccccgccac catcgccgtc gtcggcacct cgtatgtcgg
     4261 tctctcgctc gcgatgctgc tggcgcggca caacacggtg cgcgcccacg acatcgacga
     4321 gcacaagatc gcgatgctgc gcgcgggcag cagccccatc agcgaccccg acatcgagcg
     4381 cttcctcgcc gaggaggacc tcgacatcac ctacaccacc tcccccgagg aggcgtatgc
     4441 cggtgctgaa tacgtcatca tcgccacccc gaccaactac gacgaggtga ccaactactt
     4501 cgacacccgc tccgtggagt cggtcatcaa agacgtcgcg cacaccaacc ccgacgcggt
     4561 catgatcgtc aagtcgacga tcccggtcgg tttcaccctc gggatccgcg aacgcctgga
     4621 caccgacaat gtcatcttct cccccgagtt cctccgcgaa ggccgggcgc tgcacgacaa
     4681 cctgcacccc agccggatcg tggtgggcga actcagcgac cgggcccaac gcttcgccga
     4741 cctgctgcgg gacgcctccg aggagcccga cgtgccggtg ctgctgaccg gcagcaccga
     4801 ggccgaggcg atcaagttgt tcgccaacac ctacctcgcc atgcgcgtcg cctacttcaa
     4861 cgaactcgac accttcgcct ccacccacgg cctcaacacc cgccagatca tcgagggcgt
     4921 cggcctggac ccgcgcatcg gcagccacta caacaacccg tccttcggct acggcggcta
     4981 ctgcctgccc aaggacacca agcagctgct ggccaactat caggacgtcc cccagactct
     5041 gatcagcgcg atcgtcgacg ccaacaccac ccgcaaggac ttcgtggcga tggacatcgt
     5101 cgccaggcag ccgaaggtcg tcggcatcta ccggctcatc atgaaggccg gctccgacaa
     5161 cttccgctcc tcatcggtgc agggcatcat gaagcggatc aaggccaggg gcatcgaggt
     5221 catcgtctac gagcccgagt tcgcccacgg cagctggtac ggctccgagg tcgtcgacag
     5281 cctcgaggag ctgaaggccc gggccgacct gatcgtcgcc aaccgacaga cgcccgaact
     5341 cgcggacgtc gccgacaagg tctacacccg cgacctgttc ggctccgact gacgtcgatc
     5401 tcagctggag gcggggcgca gtttcgggta ctcgatcttg gggcaggtgt ccatgacgac
     5461 gtcgatgccg gccttgcggg cgcgggcggc ggcatcgggg tcgaccaccc cgagttgcat
     5521 ccagatcgcg tcgatgtcga ggcgctcctt gttggcgatg gcgtcgtcga cgaccgcgcc
     5581 gacgtgctcg ctgttgacga agcagtcgac gaccttgacg tccgtgccgt cggggatgtc
     5641 ggagatgctg gcgtagccct gctctccgtg cacggtctcg gccttggggt gcaccgggat
     5701 gatggccttg ccctgctcca tcttcaggta gcggctgatg tgccaggcat cgcgctggcg
     5761 gttggtactc aggccgacga tcacccacgt ccctggatcc acgagcaggt tccggacgag
     5821 gtcggggtcg ttctggtgcg tcatcgccac ctgttcagtg tggcacgcag tggcccgccg
     5881 cgtccgcgag cgacatccgc aatccgcgaa gaaggcgggg gcgtcaggac agctgctcgc
     5941 gcaacgcggc acctttgtcg tatgccgtct ggcgcagcga ctcctggaag gtcaccattg
     6001 cggcccgcag gcgtgcggcc tcgtcgtcgg tgccggtggc gaggatgcgg gcggcgagca
     6061 ggccggcgtt gcgcgccccg ccgatggaga ccgtggcgac gggcaccccg gccggcatct
     6121 ggacgatcga cagcagtgaa tccatgccgt cgaggtactt cagcggcacc ggcacgccga
     6181 tgaccggcaa cggcgtgacg gaggcgagca tgccgggcag gtgggccgct cccccggcgc
     6241 cggcgatgat gacgcgcagg ccgcgggcgg ccgcaccgcg cccgtaggcg atcatgtcct
     6301 caggcatgcg gtgggccgag acgacatccg cctcatacgc aatatcgaac tcctgcaacg
     6361 caatcgcggc ctgctccagg acgggccagt cactgtcgct gcccatgacg acgccgacga
     6421 cggggttcat tcggtgacca ctccttggag ataatcggcg gcatgccggg cccgctcgtg
     6481 cgcatcggca tacgactcgc tgacgacgtt gacgtgcccg atcttgcgcc cgggccgcac
     6541 gcccttgccg tacatgtgca cgcgcagtcc cgggtcacgc gccatcacgt gcttgtaggc
     6601 agggtagagg gaatcgtatt gccccccaag gacattcgcc atgaccgtca cgccgcaacg
     6661 gggtcgcggg tcaccgagcg gcaggtcgag cacggcgcgc agatgctgct cgaactggcc
     6721 ggtgatcgcg ccgtccatgc tccagtggcc gctgttgtgc gggcgcatgg cgagctcgtt
     6781 gacgacgaag ccgtcggcgg tctcgaagat ctccaccgcg aggacgccgg tgaccccgag
     6841 ctcacccgcg atccgcagcc cggcggcggt cgcgtcagcc gccagatcgg gtgacaggtc
     6901 ctttgccggc gcgtgcacct cgacgcagat cccgttctcc tgcaccgttt ccaccaccgg
     6961 ccacgccgcc gcctgccccg acggcgaacg ggcgatgagc acggcgagtt cgcgccggaa
     7021 tgcgaccagc tcctccagta gcaccggccc gcgcgccagc cactccgcca cctcctcggg
     7081 cgcggaggcg acgagaacgc ccttgccgtc atacccgccg gtgggcacct tggcgatcac
     7141 cggccagccg acggaatcac cgaaggccgc ggcctcagcg gccgtcgcca cggcggccca
     7201 gcgagggcac ggtatgccca gcgcggtcaa ccgggtgcgc atggcgagtt tgtcctgggc
     7261 aaagacgagt gcctcggggc tgggatgcag gggaaccccc gcctgcgcca acgcggagag
     7321 caccggtgcc ggcacgtgct cgtggtcgaa ggtgaccaca tcgcagtgcc gggcgaagtc
     7381 gaggaccgtc gccggatccg tgtagtcccc cacgggcgcc gagcgcacga cctgcgccgc
     7441 cgagccgtcg ccctcctcgg cgagcacgct cagcgtgacg ccgagttcgg ctgcggggcc
     7501 ggcgcacatc cgcgccagct ggccaccgcc gatgatcccg acgaccggga aaccgccggg
     7561 tgctcgaagc ggagtgggtg cactggcgtt cacccggcga ggataccggc gaccggcgcg
     7621 ctgccccgtg ccgggacggg ccgctgagac aggtaaaatc tgccggtgac gtcgcccgcc
     7681 catgcattcg tggactgggt ccgcggcacc atcaacgtct tgtggcgcga ggtcgtgaag
     7741 ttcggcatca tcggcgcgct cgccttcgtc gtcgacgtgg gcacgatgaa cctgctgcgc
     7801 aataccgtca tggaggacaa gcccacgacc gccaaggtcg tctcggcctc ggtcgcgacc
     7861 gtcttctcct ggatcggcaa ccggcagtgg accttccggg cccgccgcaa ccgccccgct
     7921 caccacgagt tcgcgctgtt cgccgtcacc aatatcgtgg ctctggtcat cggcgccggc
     7981 gcgatcgcgt tctcccacta cgggctcggc ctgaagaccc ttttcgccga caatatcgcc
     8041 aatgtcgtcg gcatcggcct cggcaccctg ttccgcttct gggcctaccg caagttcgtc
     8101 ttcgccaacg aactggaccc ggcgtcggag aggaacagcc cgaacaccgc cccctcggcg
     8161 cgcaacagtt ccagcctgcc gtagttcttc tccgccagat cgcgggccag ggccagcccg
     8221 aggccggacc ccgacgagga gacggatcgc tcgaagatgt gtcgcgcgat gcccgggtcc
     8281 acgcccgggc cctcgtcgct gacctcgacg acgacggtcg gcccgctgtg ccgggccgcc
     8341 accgaaacgg tgcccgaccc gtgggcgagc gagttctcga tgagggtgga gaggatctgc
     8401 gacagggcgt tgcgggcgat gaagacctgc agatcacgtt cgccgtgcac gcgcatgctg
     8461 cgccgggtct gggcgaaggc cggctgccac tcgcgttgca gcgcagcgag aaccgagtcg
     8521 acggacacga ccgccgcctc cggcggccgg cgcttggccc ggttcatcag gtcgtcgacg
     8581 acatcggtca ggcgggcgac ctgggcgatc gccaccccgg cctcctcctt gacgacgtcc
     8641 gggtcgtcgg tgtcggcgat ctcctccagc cgcagcatca aggccgcgag cggggtgcgc
     8701 agctggtggc tggcatcagc ggcgaagtcc cgttcggcgg ccaacgatcg cgccatgtca
     8761 ccggcactcg ccgcgatgac cgcactgacc gcatccacct ccgagacgcc ggtcgccagc
     8821 ggtgccatgt tcgactcgcc cgagccgagg cgggtcgcct gggccgcgag cgccgacagg
     8881 ggtgcggtca cccgggccgc ctggcggcgc gcgacgacca gccccaggat gctggcgaca
     8941 gccgccagcg cggcacagat gccgaagacg atgaacacga acgtggtcga cggcacccgg
     9001 tccatcaccc ggtcgcccgg ggcgcggaaa cccgcgatga cgagcagcga catgaccagg
     9061 gccgccatgg tggccaggac ggcggccacc accgacatgg tggcgcgcac gaacaggcgg
     9121 ttcacgggct actcgtcgac gtcgagccgg aacccgactc ctcgcaccgt cgagatcagg
     9181 cggggctcgt cgttgtcgcc gagcttgcgg cgcagcacgg agatgtgcac atcgagcgtc
     9241 ttggtcgagc cgtaccactc ggtgtcccag acctcgtgca tcagttgctc gcgggagacg
     9301 accttgccgc ggtcgcggtg cagcacccgc agcagggcga actccttgcc cgtgaggctg
     9361 agctcttctc cgcgcaggaa cacccgccgc gactcctcgt cgatgacgag cgcgggtacg
     9421 ttctcggggt ccgcgttgcc gcggcgcagc atcgcccggg tccgcgcgag cagttcggcg
     9481 agccggaagg gcttcttgac gtagtcgtcg gcgccggcgt cgagcccgac gacggtgtcc
     9541 acctcgtcgc tgcgggcggt caggatcagc accggggtgg tggatccggc ggcccgcagc
     9601 cgccggcaga cgtcgagtcc gtccatggtg ggcaggccga gatcgagcac gacgaggtcg
     9661 actccgcgga gggcgacctc gagcgcttcc gcaccgtcgg cgcggacttc gacctcatag
     9721 ccttcgcggc gcagggcgcg cgccaacggc tcggcgatag cctcgtcgtc ctcggcgagc
     9781 agcaccctcg tcatgtcccc atggtatgca gtgggccccc gccctcgctc cggcgtccgc
     9841 catgactcct ggtcacctcg cgtggcgtat gggtcccgat cccgcgtagc aagcggcggc
     9901 gctgggtgcg caaccggaag ccggactgct gcgccaggcg ggcggcgagc gcctcgtcgg
     9961 cgaggacgcg gccttccccg gcgaccgccg tcagccgcga ggcacggttg acggtggtgc
    10021 cgaagacgtc accgagccgg gagagcacgg gaccgtatgc cagtcccacg cgcacctgcg
    10081 gcagcacctc ctcctcggcc atcgtgtcga ccaggtcgag ggcgatcgcc gtgccggcca
    10141 ccgggtcctg gttgacgaag aggatctcgt cgccgacggt cttgatcacg cggccgccgt
    10201 gcgcggtgac gatgtcggag gcgagccgtt cgaaccgttg gacgaccacg gccagttccc
    10261 gctcggtggc ccgccgcacc accgaggtga agttgaccag gtcggcgaac cccaccagcc
    10321 gcaccggccc ggagctggcc tgcgccgggt cggcgtcgct gaccatcctc gtcagcgcgg
    10381 catacaggtg gcgccgccag gcatacacga gcaacggctc gaaggcgtcg gcggcggccg
    10441 tcaggcgacg ggcgacctcg gccgccgtct gcggatcggc gcccaagccg tccggcgaga
    10501 cgtcgggcgc gaccgactcg gcgaccagct gggtctgcca caccgacagc cggtcggcgg
    10561 tgcgggccac ggcgcgcgtc atacccaacg ccagctgttc gtccacgcca ccgtcgtcga
    10621 tgaggctgac gatccggtgc agggcaacga gatccgcttc ggtgaagact cccgagtcgg
    10681 aatgctcggc ggggaagccg agggcatgcc agaacgccgt gacgctgctc aacgccagat
    10741 cggcgcgctg agagacctcg tctctccccg tcgccaccgg cttgccgagc aatcgctcct
    10801 gaatgtggtc caggaactcc tgcacggcca gatcgtcctg ccgcatgctt ctcccgtcgc
    10861 cctccctact ctgccacgcg cacgtgatgc acgtcacctg cggcgatggc ccgcgtggcg
    10921 cccgccgggt cccggacgag caggcgcccg tcgtcgtcga caccggtggc ctccgccgcg
    10981 acgggcgcgc cctgggtctg gacggtgatc cgcgcgccga tcgtggagca ggcggcgcgg
    11041 taggcctcct gtgccgcgcg cagctcgccc ccgccggtat gcagtgcggc gagcaaggtg
    11101 gtcagctcct ggacgtaggc gatcaccaac gcgctgcggt cgatggcgcc gatcggccgg
    11161 cccagttcga gggcgagtga ggtggcggtg ggcaccggca gctcctcgcg ggtgtggtcg
    11221 acgttgaggc cggcgccgat gacgatggcg tcgggcgtca tctcgcacag gatcccggcg
    11281 agcttgcctc cggaggcggc gagcaggtcg ttgggccact tcaggtcggc acggtatgcc
    11341 gtttcgcgcg ccacggcggt gcgcagcgcg agcccggtga gcagggggac ccaacccacg
    11401 gcatgcggcg ggaccggcac tcgcagcgac atcgcgagcc cggcgcgggg agtctccgtc
    11461 cactcccgcc cgagccgtcc cttgccgccg acctggtgct cggcgagcac cagcgcgccg
    11521 ggggcgtcga gcgccagcgc ggcggtgttg gtggagtcga tctggtcgtg gcagagcatc
    11581 tgcgtgacgg aactctcccg gagggccgtc tcgattcgcg ggagatctag agaaaggcgc
    11641 atgtgggcaa ggctatgttt ggtctcatga ccgccgatgc cgccgctcag gatgtcgaca
    11701 tccacaccac cgcaggcaag cttgccgatc tcaaacgccg tgtcgccgag gtcgcgcacg
    11761 cgggtggacc ggtggccgtg gagaagcagc atgccaaggg caagatgacc gcgcgcgagc
    11821 gcattctcac cctgctcgac gagggcacgt tcatcgagat ggacaaatat gcccgccacc
    11881 gcagcaacgc cttcgggcag gagaagtcgc gcccctacgg cgacggcgtg gtcaccggct
    11941 acggcctggt cgacgggcgc acggtcgcgg tcttcgcgca ggacttcacc gtcttcggcg
    12001 gctcgctcgg cgaggtcttc ggcgagaaga tcgtcaaggt catggacttc gccatgaaga
    12061 tcggctgccc ggtcgtcggg ctcaacgact ccggcggcgc gcgcatccag gagggcgtcg
    12121 tctcgctcgg cctctacggc gagatcttcc tgcgcaatgt ccacgcctcg ggcgtcatcc
    12181 cgcagatctc gctgatcatg ggcccctgcg ccggcggcgc ggtctactcc cccgcgatca
    12241 ccgacttcac gatcatggtc gacaagacct cgcacatgtt catcaccggc ccggacgtga
    12301 tcaagaccgt caccggcgag ga
//
LOCUS       DAOPJG010000094        12279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5428_length_12279_cov_7.332215, whole genome shotgun sequence.
ACCESSION   DAOPJG010000094 DAOPJG010000000
VERSION     DAOPJG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12279
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5428_length_12279_cov_7.332215"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1516
                     /locus_tag="PKU75_09615"
     CDS             191..1516
                     /locus_tag="PKU75_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HPF81176.1"
                     /translation="MRSVSAVDNRPLSDLSPAELNDFLAAKRAAYQALQGRGLKLDLT
                     RGKPSAAQLDLADGLLKLPTSISDADGVDVRNYGGLTGIRQIREIFAELLWVEPEQVI
                     AGGNSSLTMMHDCLVQMLLHGPVDGQPWVGQPIKFVCPVPGYDRHFTMLASYGIEMLP
                     VPMEADGPDVAAIEALVKDDPMVKGMWIVPTYANPVGSTISHEKAALLAAMPTAAADF
                     KIFWDNAYAFHHLTEEEAKSEDIISLCSAAGNPNRPIMFASTSKITYAGAGVAVLAAS
                     SANVGWYQKHYQYASIGPDKVNQLRHVQFFTNAQGVRDHMVKHREILAPKFAAVDAAL
                     TAELAGLGVAEWTKPTGGYFVNLDVVDGTAGRVVQLAKEAGIVLTPAGASFPHGHDPR
                     DRNIRLAPSFPVLEEVETAMAGVATCVALAAAEELSGRHTASASTPEGH"
     gene            1586..2098
                     /locus_tag="PKU75_09620"
     CDS             1586..2098
                     /locus_tag="PKU75_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HPF81177.1"
                     /translation="MSEQNEGINDDFGTYSLDDEDQLQPEDTLIDDGVEDVLDRGYSP
                     ADTDRGSRAFGVTAEEQSEHETIDQRIRQEVPDPNSAYGAPYHESVLDEEFVGGDDPD
                     HIAAEDDWLGDEEVGDVRAGRLVAPDQGGSGDHDHEMIGSDVGIDGAGASAEEAAMHI
                     ISGDDLRYED"
     gene            2113..3165
                     /locus_tag="PKU75_09625"
     CDS             2113..3165
                     /locus_tag="PKU75_09625"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPF81178.1"
                     /translation="MAALPKAHLHLHFTGSLRLSTLVELAGAHGIRLPHALTGEWPPQ
                     LASVQDERGWFRFQRLYDAARACVRGEADMRRLVREASLDDAAEGSRWLELQVDPTSY
                     APYVGGLTPALEIVLDEAAAVSREGGAPMGIMVAASRIRHPLDARTLARLAARYAGSG
                     PGTVVGFGLSNDERRGDTTEFAVAFDIARKAGLALMPHAGELLGPTAVEETLDALRPN
                     RIGHGVRSVEDPRVVERLASDGVALEVCPGSNVALGVYREADEVPVRTLLRAGIPIAL
                     GADDPLLFGSRLVDQYALARDELGFSDQELAELARGSIRASYAPQDIKAAALGDIDAW
                     LATGPPAGSDAPHPQG"
     gene            3229..4251
                     /locus_tag="PKU75_09630"
     CDS             3229..4251
                     /locus_tag="PKU75_09630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HPF81179.1"
                     /translation="MTQFGYFLSSEEYTPAELIEQARLAEEAGFDALWISDHFHPWTD
                     AQGQSPFVWSMIGAISQVCDLPVTTAVTCPTVRTHPAIIAQAAATSAALLGPGRFRLG
                     LGTGEALNEHITGEVWPHAQQRLDMLEEAIAVIRELWTGEVVSHEGRFYTVDTARIYT
                     LPSEPIPIYVSAFGPKALDLVCDVADGFITTQPDADSVKTFKKAKGAGAPTQAGFKVA
                     WGPSRDEAIDAAYRLWPNSGVPGELAQVLPSPQHFEQAAKLVTREKFAEQGAFGPDPD
                     DHIAAFAPYVEAGFDEIYVANMGPHYAELMSAYGESVLPQLRARKGKRAAAKKTTAKR
                     SSRSKR"
     gene            4300..4911
                     /locus_tag="PKU75_09635"
     CDS             4300..4911
                     /locus_tag="PKU75_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPF81180.1"
                     /translation="MTYDIPRPTGGDIVELILDDHRLFEDLLRECRRDDRDRAAAREA
                     LAELLVAHAEAEEAEVYPKLHSKRAITAHEEEHGEEEHAEITEALLAFVEAKGTDTQK
                     YEDALEKLAKVVNHHSCEEELTILNPARDEVSDSVRHDVGVAWATMRNTLLENGCASR
                     DQVAALLRKAEKEGAIPPPAVRERQEAIEEDAKKRADALAEDA"
     assembly_gap    5083..5182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5183..6145)
                     /locus_tag="PKU75_09640"
     CDS             complement(<5183..6145)
                     /locus_tag="PKU75_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1704 domain-containing protein"
                     /protein_id="HPF81181.1"
                     /translation="MTSPAVELSPTDLAVDHALAHLSSGRRFLLEVTPVDADEARAAY
                     LDGSAPDPQFTYRELDADPDVTAAQLAAINVTNVENLTLGHLLRAKHRELELQVEMLR
                     ARDSEDFLGLSIELYGSVSPTLRAQAEQVLAAVLAAESAGSALHAEAFLLLAQEEIAH
                     YREQDPDIEMHAEIRFDAVGVMVSGDTLLIGPDSAVQQRRAAALIHHEVGTHLVTQVN
                     GAAQPSKVLGTGLAGYDETQEGLAVVAEIACGSLTAFRLRQLASRVLTVDRRVAGASF
                     HEAHEALVSGGIPPGSAFTTVMRVFRAGGMTKDAIYLRGLVDLLA"
     gene            complement(6425..6772)
                     /locus_tag="PKU75_09645"
     CDS             complement(6425..6772)
                     /locus_tag="PKU75_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPF81182.1"
                     /translation="MIGDGGLAVHLGELAVLAAEQPNVTVVLFNDGGYGVLRNLQEAG
                     GRTRRGVDLHTPDFAALSAAFGLRHKLIASAAEFDKALKKAVGRPGPTVIEVDVAALR
                     PRPRRMVPPVSIP"
     gene            complement(6769..8136)
                     /locus_tag="PKU75_09650"
     CDS             complement(6769..8136)
                     /locus_tag="PKU75_09650"
                     /inference="COORDINATES: protein motif:HMM:NF005470.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPF81183.1"
                     /translation="MGKRDQGKAPKRHTTTETHEAPTSDLTAPARTGGDILVGVLREA
                     GVHAAFGVVSIHNLPLVEAVDRDLRFVPMRHEAAAVNAADGYARASGGVGVALTSTGT
                     GAGNAAGSMLEALTAQSRVLHVTGNIDADLLGEHRGAYHEVPRQLQMLDAVSAQALRI
                     QTARQARAVLTDAVQRLLTPPQGPVSVDWPIDLQYTADPDAQNTVPRPPRQPPAGTRR
                     DIERAAAVMRQAKRPLIWAGGGARRVGAPLAALTQAWGAGVLTGANGRGAVPEDHLQC
                     VGNFATHDALAELIEDADCLLTIGSHLRANETRNFELPLPSTHIQIDVDEQAIGRNYP
                     VTVGVRAEASRAVPALLAALGDVETDAAWTDRISAAAGAARAAHRADIGAYSDICDAI
                     RNRLPRNSPIVRDVTIPGSSWGNRLLPVFDPTDNVYAAGGGIGQGLAMAIGAAIARPD
                     DRVPP"
     gene            complement(8150..8311)
                     /locus_tag="PKU75_09655"
     CDS             complement(8150..8311)
                     /locus_tag="PKU75_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81184.1"
                     /translation="MSDAMHEAMTDQTTALNACDCPNCEVGCSCNDCECTDCTCPRCS
                     HDQDEELED"
     gene            8604..9827
                     /locus_tag="PKU75_09660"
     CDS             8604..9827
                     /locus_tag="PKU75_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPF81185.1"
                     /translation="MTSAAPTSTNRPTAQVVWGVAEPAPLTGVTLTPDGSSVIYFAEA
                     GTKRSLRALEARTGKVIWEADLSDRGSAVNQTITPPVVVGDSVAAVIADGEGGRIRVF
                     DVRTGARVGRDSPVAVEIDQLGPCPDGRAWCFIGDTRMHHPLPTLRYAPGDRSFSPAK
                     AGLVVVNPVADPFEYIVDGEVQWTLDPQSIMKTCPRYVCMRGWATIGEVTAMGFRESG
                     KGGHLVSTTVAAFTTRTGRKRWVSPGDQLAQVATQSGHFSHEGDVYVFTPEPARVNGT
                     YDDFDGRLLYGGRLRALDPETGKERWSVALGRSRFQPRLTRLSNGDLIFDEGNQTRLL
                     DAASGDVIADRDLSYWRSSTDGPPGRRARLDITEPMIDAGPSDTVRKPIPDEIGVQAG
                     DLRIVAQPNGVYAYR"
     gene            complement(9824..10912)
                     /locus_tag="PKU75_09665"
     CDS             complement(9824..10912)
                     /locus_tag="PKU75_09665"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPF81186.1"
                     /translation="MPEEFDAPLAPLTTMRVGGPAARLVTVTTTDELVDAVREVDDAG
                     EPLLVVGGGSNLVIADEGFAGTVVHVRTTGVTRESQDYCGGAFVRIAAGEVWDDFVAR
                     AVAEGWAGIEALSGIPGSAGATPIQNVGAYGQEVAQTIAQVRTWDRETQSVRTFAANE
                     CAFTYRHSVFKASTRYVVLDVAFQLEVADLSRPIAYADLAAGLGVEQGARVPLADARE
                     AVLAQRRKRGMVLDPDDHDTWSCGSFFTNPVLSQRAFDRLFERVHERLGSDVSPPAFP
                     GADGSVKTSAAWLIDKAGFSKGFGAARESGLSGRATLSTKHTLAVTNRGDATAADILA
                     LARTVRDGVHDAFGVTLVNEPVFIGHQL"
     gene            complement(10912..11331)
                     /locus_tag="PKU75_09670"
     CDS             complement(10912..11331)
                     /locus_tag="PKU75_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPF81187.1"
                     /translation="MARAFASVSVGEELGPKTMPVGRGTLVDYANASLDQNPIHQDEG
                     FAKSVGLPDVIAHGMWTMGAAATLVSDWAGDAGRVVEYGTRFTAMVVVPLDGAELEVS
                     GVVKSLDEATKRATVELTATAAGQKVLGRCTAVVQLD"
     gene            complement(11334..11777)
                     /locus_tag="PKU75_09675"
     CDS             complement(11334..11777)
                     /locus_tag="PKU75_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPF81188.1"
                     /translation="MPVNEDYVGRSFPPVGPYAVDPAMIAAFASAVGATDPVHFDAEA
                     ARAAGYRDVIAPPTMAVRFDQLASREYVQDPDAGIDYSRVVHGEQRFTHHRPITAGDE
                     LTAQTTVDSIRMAGGHAMITVRSEITAGEEPVATTHSTIVVRGGE"
     gene            complement(11947..12117)
                     /gene="rpmG"
                     /locus_tag="PKU75_09680"
     CDS             complement(11947..12117)
                     /gene="rpmG"
                     /locus_tag="PKU75_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPF81189.1"
                     /translation="MAKKTADIRPKITLACTECKERNYITEKNRRNTPDRVELAKFCP
                     RCRKHTPHRETR"
BASE COUNT         1915 a         4100 c         4299 g         1865 t
ORIGIN      
        1 cccccaaccg ccggttttgg agaccggtgc tctaccaatt gagccactgc cctagggaaa
       61 cgcgcgggtt gaggcgagcg ttcccagccg gtgacgatgg gcatgctcac gcacggccac
      121 gatcggcacc gagggaacag cataccggcg ttttgggacg acaggaaact cgattgccgt
      181 cggtgggagg atgcggagcg tgagcgcagt cgacaaccgg cccctgtccg acctctcccc
      241 cgcggaactg aacgatttcc tcgcggccaa gcgcgcggca taccaagccc tgcaaggacg
      301 cggactcaag ctcgacctga cgcgcggcaa gccgtcggcg gcgcagctcg acctggccga
      361 cgggctactg aagctgccga cgagcatcag cgatgccgac ggtgtcgacg tgcgcaacta
      421 cggcgggctg accggcatcc ggcagatccg ggagatcttc gccgagctgc tgtgggtgga
      481 gccggagcag gtgatcgcgg gcggcaactc cagcctgacg atgatgcacg actgcctggt
      541 gcagatgctg ttgcacgggc cggtcgacgg gcagccgtgg gtggggcagc cgatcaagtt
      601 cgtctgcccg gtgcccgggt atgaccggca cttcacgatg cttgcgtcct acggcatcga
      661 gatgctgccg gtgccgatgg aggccgacgg gccggatgtc gcggcgatcg aggcgctcgt
      721 caaggacgat ccgatggtca agggcatgtg gatcgtcccg acctatgcca acccggtcgg
      781 gtcgacgatc tcgcacgaga aggctgcgtt gctggcggcg atgccgacgg cggccgccga
      841 tttcaagatc ttctgggaca acgcctacgc cttccatcac ctgacggagg aggaggccaa
      901 gagcgaggac atcatctcgc tgtgctcggc cgccgggaac ccgaaccggc cgatcatgtt
      961 cgcctcgacg tcgaagatca cgtatgccgg ggcgggggtc gcggtgctcg ccgcgagttc
     1021 ggcgaatgtc ggctggtatc agaagcacta tcagtacgcc tcgatcgggc cggacaaggt
     1081 gaaccagctg cggcacgtgc agttcttcac caacgctcag ggcgtgcgcg accacatggt
     1141 caagcaccgc gagatcctgg cgccgaagtt cgcggccgtc gacgccgcac tgacggccga
     1201 actcgccggg ctgggggtcg cggagtggac caaaccgacc ggcggttact tcgtcaacct
     1261 cgacgtcgtc gacggcaccg ccggacgcgt ggtgcagctg gccaaggagg ccgggatcgt
     1321 cctgacgccg gccggcgcgt ccttcccgca cgggcacgac ccgcgcgatc gcaatatccg
     1381 cctcgccccg tccttccccg tgctcgaaga ggtcgagacg gcgatggcgg gcgtcgcgac
     1441 ctgcgtggcc ctggccgcgg cggaggagct gagcgggcgg cataccgcca gcgcgagcac
     1501 ccccgagggc cactgagggc cgacgtctcg cagaccacac actcctgtgg ctgatgtgac
     1561 ggcgcggggt gattaggatt ccggtatgtc ggagcagaac gaaggaatca acgacgattt
     1621 cggcacctac agcctcgacg acgaggatca actccagccg gaggacaccc tcatcgacga
     1681 cggtgttgag gacgtgctgg accggggtta ctcccctgcc gacaccgacc gcggctcccg
     1741 cgccttcggc gtgaccgccg aggagcagag cgagcacgag acgatcgacc agcggatccg
     1801 gcaggaagtc cccgacccca actcggccta cggagcgccc taccacgagt cggtgctcga
     1861 cgaggagttc gtcggcggcg acgacccgga tcacattgcg gccgaagacg attggctcgg
     1921 cgacgaagag gtcggcgatg tgcgcgccgg ccggctggtg gcgcccgatc agggcggctc
     1981 gggggaccat gaccacgaga tgatcggctc ggacgtcggc atcgacgggg ccggggcgtc
     2041 ggccgaggag gccgcaatgc acatcatcag cggggacgac ctgaggtacg aggactgaac
     2101 gccgggcgcg acctggcggc gctgccgaag gcgcacctgc acctgcactt caccggctcg
     2161 ctccggctgt ccacgctcgt ggaactcgct ggggcacacg gcattcggct cccccacgcg
     2221 ttgacgggcg agtggccgcc acagctggcg agcgtgcagg acgaacgtgg ctggttccgc
     2281 ttccaacggc tgtatgacgc cgctcgcgcc tgcgtgcggg gcgaggcgga catgcggcgg
     2341 ctggtgcgtg aggcgtcgtt ggacgacgct gccgagggct cgcggtggct ggagttgcag
     2401 gtggacccca cgtcgtatgc gccgtatgtc ggcggtctga cgccggcgct ggagatcgtg
     2461 ctcgacgagg cggctgccgt ttctcgcgag ggcggcgcac cgatgggaat catggtggcg
     2521 gccagccgga ttcgccaccc gctggatgcc cgcacgctcg cgcggctggc cgcccggtat
     2581 gccgggtccg ggcccggcac ggtggtcggg ttcgggttga gcaacgacga acgccgcggc
     2641 gacacaacgg aattcgcagt cgccttcgac atcgcccgca aagctgggct cgccctcatg
     2701 ccgcacgcgg gtgagttgct cggaccgact gccgtcgagg agacgctgga tgccttgcgc
     2761 cccaaccgga tcgggcacgg ggtgcggtca gtggaggatc cgcgggtggt ggagcggttg
     2821 gcgagcgacg gtgtggcgct ggaggtctgc cccggcagca atgtcgcgct cggggtctac
     2881 cgggaggccg acgaggtgcc ggtgcgcacc ctgctccgcg ctggcatacc cattgccctg
     2941 ggggccgacg accccctgct gttcggctcc cggctcgtgg atcaatacgc gttagcccgc
     3001 gacgagctcg gcttctccga ccaggaactc gccgaactgg cccgcgggtc gatccgcgcc
     3061 tcatacgccc cgcaggacat caaggccgcc gccctcgggg acatcgacgc ctggctcgcg
     3121 accggtccgc cggcgggctc ggacgcacct caccctcagg ggtgagaaac gaatcgggtt
     3181 gcccgccaac ggcgctgggt ccgggcacgc ggccgtactg ttcgggacat gacccagttc
     3241 ggatacttcc tctcgagcga ggagtacacc ccggcggagc tgatcgagca ggcacgactg
     3301 gccgaggagg ccggtttcga tgcgctgtgg atcagtgacc acttccatcc ctggaccgac
     3361 gcgcaggggc agtcgccctt cgtctggtcg atgatcgggg ccatcagcca ggtctgcgat
     3421 ctgccggtga ccacggccgt cacctgcccc acggtgcgga cccatccggc gatcatcgcc
     3481 caggccgcag cgaccagcgc ggcactcctc ggcccgggcc gtttccgcct cggtctcggc
     3541 accggcgaag cgctcaacga gcacatcacg ggcgaggtgt ggccgcacgc gcagcagcgg
     3601 ctggacatgc tcgaggaggc gatcgcggtg atccgcgaac tgtggaccgg cgaggtcgtc
     3661 tcgcacgagg gccgcttcta caccgtcgac acggcacgca tctacaccct tcccagcgag
     3721 ccgatcccga tctacgtcag cgctttcggg cccaaggccc tcgacctcgt gtgcgatgtc
     3781 gccgacggct tcatcacgac ccagccggat gctgacagcg tcaagacctt caagaaggcc
     3841 aagggcgccg gcgcccccac ccaggcgggg ttcaaggtcg catgggggcc gtcacgcgac
     3901 gaggcgatcg acgcggcata ccggctgtgg cccaacagcg gcgtgccggg agaactggcg
     3961 caggtccttc ccagcccgca gcatttcgaa caggcggcga agctggtcac ccgggagaag
     4021 ttcgccgagc agggcgcctt cgggcccgac cccgacgatc acattgctgc cttcgccccg
     4081 tatgtggagg ccggtttcga cgagatctat gtggcgaaca tgggtccgca ctatgccgag
     4141 ctgatgtccg cctacggcga gagcgtcctc ccgcagttgc gggcgcgcaa gggcaagcgc
     4201 gccgcggcga agaagacgac cgctaagcgg tcgtcacgct ccaagcgctg acatcatcac
     4261 tcacgacgct cccgcaacca gccccccagg agtagtgcca tgacctacga catcccccgt
     4321 cccaccggcg gcgacatcgt cgaactgatc ctcgacgatc accgtctttt cgaggacctg
     4381 ctgcgcgaat gccggcgaga cgaccgcgat cgcgccgccg cccgcgaggc gctcgcggaa
     4441 ctgctcgtcg cgcacgccga agccgaagag gccgaggtct acccgaaact gcacagcaag
     4501 cgggccatca ccgcccacga ggaggagcac ggcgaggagg aacacgccga aatcaccgag
     4561 gcgctgctcg ccttcgtgga ggccaagggc accgacacgc agaaatacga ggacgccctc
     4621 gaaaagttgg ccaaggtcgt caaccaccac tcctgcgagg aggagctgac catcctcaac
     4681 ccggcgcgcg acgaggtctc cgacagcgtg cgacatgacg tcggcgtcgc gtgggcgacc
     4741 atgcggaaca cgctcttgga gaacggttgt gcctctcggg accaggttgc ggcgctgctg
     4801 cgcaaggccg agaaggaggg ggccatcccg ccgcccgccg tgcgcgagag gcaggaggcc
     4861 atcgaagagg acgcgaagaa gcgggccgac gcgctggccg aggatgccta aaggtccggg
     4921 gccccctgag gtccgaggac gcctaaaggt ccgccgacca ctggccccag tgtcagccgg
     4981 tggccagcgc gcggttgtcg atcgagacgc ccttcggcgc ggaggtgggc gagcaggtcg
     5041 accagcccgc gcaggtagat cgcgtccttg gtcatgccgc ccnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnggcgagca ggtcgaccag cccgcgcagg tagatcgcgt
     5221 ccttggtcat gccgcccgcg cggaagacgc gcatgaccgt cgtgaaggca ctgcccggcg
     5281 gtatgccgcc gctcaccaga gcttcgtgtg cctcgtggaa gctggcgccc gccacgcgcc
     5341 ggtcgacggt gagcacgcgc gatgccaact gccgcaaccg gaatgccgtc aagctgccgc
     5401 aggcgatctc ggcgacaact gccaggccct cctgggtctc gtcatagccg gcgagcccgg
     5461 tgccgagcac cttgctcggc tgtgcggcac cgttgacctg cgtgacgagg tgggtgccga
     5521 cctcgtgatg gatgagcgcg gcggcgcggc gttgctgcac ggccgagtcg ggcccgatga
     5581 gcaaggtgtc gcccgagacc atgaccccca cggcgtcgaa acggatctcc gcgtgcatct
     5641 cgatgtcggg gtcttgctcc cgatagtgcg cgatttcctc ttgtgcgaga aggagaaagg
     5701 cttcggcgtg cagggccgag cctgccgact cggccgcgag taccgcggcg agcacctgct
     5761 cggcctgcgc gcgcagcgtc ggcgagacgc tgccatacag ctcgatcgac aacccgagga
     5821 agtcctcgct gtcgcgcgcc cggagcatct cgacctgcaa ctccaactcg cggtgcttgg
     5881 cgcgcaacag gtgaccgagg gtgaggttct cgacgttcgt gacgttgatg gccgcgagtt
     5941 gggcggcggt gacatccggg tcggcgtcga gttcgcgata ggtaaactgc gggtcgggtg
     6001 cgctgccgtc gaggtatgcc gcgcgcgcct cgtcggcgtc gaccggggtc acttcgagca
     6061 agaagcgcct gccggaggaa aggtgcgcca gggcgtggtc gacggccagg tcggtggggc
     6121 tgagttcgac ggcgggagat gtcacggcag ccggtccagc tcggtttcga tgcccggcag
     6181 ggttgcggcg agattcgctg tcgcgcaggc ggatgcggtc gtgaaggggt cttcctccta
     6241 gaaccggtcc gcctccggga gcaccatggc ggccgcaaga tcggggcgta cgtcgtgccc
     6301 cgcgtgcacc gaggccgcca cgatccggcc agtccacggg cctatgaatg cgaccacctc
     6361 gtcgtgcctc atggcccttt tgtaccgccc gggcagccca gcgggctgct atgacctggt
     6421 gcttctaggg gatgctcacg ggagggacca tgcgcctcgg ccggggccgc agcgccgcca
     6481 cgtcgacctc gatgacggtc gggcccggcc ggcccacggc cttcttcagc gccttgtcga
     6541 actccgcggc gctggcgatc agcttgtgcc gcagcccgaa cgccgcggac agcgccgcga
     6601 agtccggggt gtgcaggtcg accccgcgcc gggtccggcc gccggcctcc tgcagattgc
     6661 gcagcacgcc gtagccgccg tcgttgaaga gcacgacggt gacattgggc tgctccgcgg
     6721 cgagcacggc cagctccccg agatgcacgg ccaggccgcc gtcgccgatc atggcggcac
     6781 ccggtcgtcg ggccgggcga tcgcggcacc gatggccatg gccaggccct gaccgatgcc
     6841 gccgccggcg gcgtacacat tgtccgtggg gtcgaagacg ggcagcagcc ggttgcccca
     6901 ggaactgccg gggatcgtga catcgcgaac gatgggggag ttgcgcggta gccggttgcg
     6961 tatggcgtca cagatgtcgg agtaggcgcc gatgtccgcc ctgtgtgcgg cccgggcggc
     7021 gcccgcggcc gcgctgatcc ggtcagtcca cgccgcgtcc gtctcgacgt cgcctaatgc
     7081 cgcgagcagg gccggcacgg cccgcgacgc ctcggcgcgc acgccgacgg tgaccgggta
     7141 gttgcggccg atcgcctgct cgtcgacatc gatctggatg tgcgtgctcg gcaggggcag
     7201 ctcgaagttg cgagtctcgt tggcgcgcag atgacttccg atggtcagga ggcagtcggc
     7261 gtcctcgatc aactccgcca gtgcgtcgtg tgtggcgaag ttgcccacac actggaggtg
     7321 gtcctccggc acagcgccgc gcccgttggc tcccgtcaat acgcccgctc cccacgcctg
     7381 cgtcaacgcg gcgaggggtg caccgacccg gcgcgctccg ccgccggccc agatcaacgg
     7441 ccgcttggcc tgacgcatga ccgcagccgc ccgttcgatg tcgcggcggg tgccggccgg
     7501 cggctggcgc gggggccgcg gcacggtgtt ctgcgcatcg ggatcggcgg tgtactgcag
     7561 gtcgatgggc cagtcgacgc tgaccggtcc ttgcggtggc gtcagcagtc gttggacggc
     7621 gtcggtcagg acggcgcgag cctggcgtgc ggtctggatt cgcagcgcct gggccgatac
     7681 cgcatcgagc atctgcaact gcctcggcac ctcgtgataa gcgccgcggt gctcgccgag
     7741 caggtcggcg tcgatattgc cggtcacgtg caggacccgg ctctgcgcgg tcagcgcttc
     7801 gagcatggac ccggcggcat taccggcgcc ggtcccggtc gaggtgaggg cgaccccgac
     7861 gccgccgctc gcgcgggcat aaccgtcggc cgcgttgaca gctgccgcct cgtgacgcat
     7921 cgggacgaac cgcagatcgc ggtcgacggc ctcgacgagc ggcaggttgt ggatgctgac
     7981 gaccccgaac gccgcatgga cgcccgcctc gcgcagcacg ccgacgagga tgtcgccgcc
     8041 ggtccgcgcg ggggcggtca ggtcgctcgt cggcgcttcg tgggtctcgg tcgtcgtatg
     8101 ccgcttcggc gcctttccct gatcccgttt gcccatcccg cgcgggtcct cagtcctcga
     8161 gctcctcgtc ctggtcgtgg gagcacctgg ggcacgtgca gtccgtgcac tcgcagtcgt
     8221 tgcagctgca tcccacctcg cagttgggac agtcacaggc gttgagggcc gtggtctgat
     8281 cggtcatcgc ctcgtgcatt gcgtcgctca tcgagcctcc tccgctggcg gcgaccactg
     8341 cgtcgccgtg cgtcgagtct gacagccaga gagcggaaag tgtccgccac cgcctgtgga
     8401 aaactgcttc tcgactagcg agactcgatc atctgaccta cgttcgaggc atgtcaacgc
     8461 cgcagggggt cgtcgtgcaa cggggtcctc gatccgccgc ccgcgcccgc atcctgtgcg
     8521 gaatcgtcgt gctcaccgct gggagcgcct gctctcaaca ggcacgtcca accgcctcgc
     8581 caacgacttc cgccagtttc accatgacgt cggcagcgcc gacgtcgacc aaccgaccca
     8641 ctgcgcaggt ggtctggggg gtcgcggaac cggcgccgct caccggcgtc acgctgacgc
     8701 cggatggcag cagcgtcatc tatttcgcgg aggccgggac gaagcgatcg ctgcgggctc
     8761 tcgaggcccg gaccggaaag gtgatctggg aagcggattt gtccgaccgg ggatccgcag
     8821 tcaaccagac catcacaccc cccgtcgtag tgggcgactc cgtggccgcg gtcatcgccg
     8881 atggggaagg cggccgaatc cgggtcttcg acgtgcggac gggcgcccgg gtcggccgcg
     8941 attctcccgt cgccgttgag atcgaccagt tgggcccctg ccccgacggc cgggcgtggt
     9001 gcttcattgg cgatacccgg atgcaccatc ccttgccgac ccttcgctac gcacccggcg
     9061 atcggtcctt ctcccccgcg aaagccggac tcgtcgtcgt caatcccgtc gcggacccct
     9121 tcgaatacat cgtggacggt gaggtccagt ggaccctgga tccccaatcg atcatgaaga
     9181 cctgtccccg atatgtgtgc atgagagggt gggcgacgat cggcgaggtc accgcgatgg
     9241 gtttccgcga atccggcaag ggcggccacc tcgtgtcaac gacggtcgcg gccttcacca
     9301 cccgcaccgg cagaaagcgc tgggtgtccc cgggagacca gctcgcccag gtcgcgaccc
     9361 aatccgggca tttcagtcac gagggtgatg tctacgtctt tacaccagaa cctgcccgcg
     9421 tcaacggaac ctatgacgac ttcgatggac gcctcctcta cggcgggcga ctgcgggcgc
     9481 tcgatccgga aactggcaag gagcggtgga gcgtggcact ggggcgctca cgctttcagc
     9541 cccgtctgac tcgcctgtcc aacggcgacc tgatcttcga cgagggcaac cagacgcggc
     9601 tgctggacgc agccagcggg gacgtgatcg ccgatcgaga cctctcctat tggcgcagta
     9661 gcacggacgg cccgcccgga cggcgggccc gcctcgatat caccgagccg atgatcgacg
     9721 caggcccgag tgacaccgtg cgcaaaccca tcccggacga gatcggggtg caggccggag
     9781 acctgcggat cgtggctcaa cccaatggcg tctacgcata tcgctagagc tggtggccga
     9841 tgaagacggg ctcattgacg agggtgacgc cgaaggcgtc gtggacgccg tcgcggaccg
     9901 tgcgggcgag ggcgaggatg tcggcggcgg tggcgtcgcc gcggttggtg acggctagcg
     9961 tgtgcttggt cgagagtgtg gcgcgaccgc tcaggcccga ctccctcgcg gctccgaagc
    10021 ccttgctgaa gccggccttg tcgatcagcc aggcggcgct ggtcttgacg ctgccgtcgg
    10081 cgccggggaa agcgggtggg ctgacgtcgg agcccagtcg ttcgtgcacc cgctcgaaaa
    10141 gccggtcaaa agcccgctgg gagagaacgg gattggtgaa gaacgacccg cacgaccagg
    10201 tgtcgtggtc gtccgggtcg agcaccatcc cgcgcttgcg gcgctgcgcc agcacggcct
    10261 cccgggcgtc ggcgagcggc acccgcgcgc cctgctcgac gccgagccct gcggccagat
    10321 cggcatacgc gatcggccga gagaggtccg ccacctcgag ttggaacgcg acatcgagca
    10381 cgacatagcg cgtgctggcc ttgaacacgg aatgccggta ggtgaacgcg cattcgttcg
    10441 cggcgaaggt gcgcaccgat tgggtctcgc ggtcccaggt gcgcacctgc gcgatggtct
    10501 gggcgacctc ctggccgtag gcgccgacat tctgtatcgg ggtcgccccc gcggagccgg
    10561 gtatgccgct cagggcctcg atcccggccc acccctcggc gacggcgcgg gcgacgaagt
    10621 cgtcccagac ttcgcctgct gcgatccgga cgaaggcgcc gccgcagtag tcctgcgact
    10681 cccgcgtcac gccggtggtc cggacgtgga cgacggtgcc ggcgaatccc tcgtcggcga
    10741 tgacgaggtt ggagccgccg ccgacgacca gcagtggctc gccggcgtcg tcgacctcgc
    10801 ggacggcatc gacgagttcg tcggtagtgg tgacggtgac caaacgcgcg gccggcccgc
    10861 cgacacgcat ggtggtcagc ggcgccaggg gtgcgtcgaa ctcctctggc atcagtcgag
    10921 ctggacgacc gcggtgcacc ggccgaggac cttctgcccg gcagcggtcg cggtcagctc
    10981 gacggtggcg cgcttggtcg cctcgtcgag ggacttgacg accccggaga cctccagctc
    11041 ggccccgtcc agcggcacga cgaccatggc cgtgaaccgc gtgccgtatt cgacgacccg
    11101 tcccgcgtcg cctgcccagt ccgagacaag cgtggcggcg gcgcccatcg tccacatgcc
    11161 gtgggcgatg acgtcgggca ggccgacgct cttggcgaag ccctcgtcct ggtggatggg
    11221 gttctggtcg agggaagcgt tggcgtagtc gaccagcgtg ccgcggccga cgggcatggt
    11281 cttcgggccg agttcctcgc cgacactgac cgaagcaaat gcgcgggcca tggtcactcc
    11341 cctccccgca cgacgatcgt cgagtgcgtg gtcgcgaccg gctcctcgcc ggccgtgatc
    11401 tcgctgcgca cggtgatcat ggcgtgcccg ccggccatcc ggatcgagtc gacggtggtc
    11461 tgtgcggtca gctcgtcgcc ggcggtgatc gggcggtggt gggtgaagcg ctgctcgccg
    11521 tgcacgacgc gggagtagtc gatgccggcg tccgggtcct ggacgtactc gcgagaggcc
    11581 agctggtcga agcgcaccgc catcgtcggc ggggcgatga catcccgata gcccgcggcg
    11641 cgggccgcct cggcgtcgaa gtgcaccggg tcggtcgcgc ccacagcgga cgcgaacgcc
    11701 gcgatcatgg ccgggtccac ggcatacggc cccacaggcg ggaagctgcg ccccacgtag
    11761 tcctcgttca ctggcatacc ggcgagtcta ggcgctgggc tttgggaggg tttgtggcgg
    11821 ttcgcctcgc gaacgttgtc aggcgaaccg acaggaagtg gcactgggcg gaaaagtgcg
    11881 gttgtcaccg acacgccgaa gcgccccgac ccagctcggg gtcggggcgc ttcggggcgc
    11941 gaaacgtcag cgggtctcgc ggtgcggggt gtgcttgcgg caacgcgggc agaacttggc
    12001 cagctcgacc cggtcagggg tgttgcggcg gttcttctcg gtgatgtagt tgcgctcctt
    12061 gcactcagtg cacgccaagg tgatcttggg gcggatgtcg gcagtcttct tagccacgga
    12121 gcaacctgct tctaggggta cggacggatt cagcctgcga tcgggtcgca ggacaccgac
    12181 acaacagaat accgaggctt cgcattcccg cgaaatcctc gggtggtagc gggagcgggg
    12241 ctcgatcccg cgacctcacg attatgagtc gtgcgctct
//
LOCUS       DAOPJG010000095        12235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5446_length_12235_cov_7.300821, whole genome shotgun sequence.
ACCESSION   DAOPJG010000095 DAOPJG010000000
VERSION     DAOPJG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5446_length_12235_cov_7.300821"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..546)
                     /locus_tag="PKU75_09685"
     CDS             complement(175..546)
                     /locus_tag="PKU75_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPF81190.1"
                     /translation="MTSPAPHLLVKLTCGEEALERANQALTVAATAVASGAEVSLWLT
                     GEASWLAVPGRAEALTLPHAANPAELLEAVLAAGQVTVCSQCAARRDLTAEALRPGVA
                     IRGAAAFVEEALTEGTQALVY"
     gene            570..1061
                     /locus_tag="PKU75_09690"
     CDS             570..1061
                     /locus_tag="PKU75_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HPF81191.1"
                     /translation="MFTLDESIHEQVRPLAWLIGRWEGAGVVGYPTMESRNFGQEIVV
                     EHDGRPFLRWQTRSWLLNEDGAIDRPASTEMGFWRMAGDEAELLLAHPTGILELYFGP
                     VEPARISLKTDGVLRSAHAKEYNAATRLYGLVNSNLMWAMDMAAVGTPLTSHASAELK
                     RVE"
     gene            complement(1081..2097)
                     /locus_tag="PKU75_09695"
     CDS             complement(1081..2097)
                     /locus_tag="PKU75_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPF81192.1"
                     /translation="MSHSLPDQASPPRTRRRGRRILAVLLGLALLAVGAMGGFVLYLN
                     NRISHIAVDDTLLPPDAAQGNDNPGTDPADGESEPLVESPGTNYLIIGSDARPGDAGR
                     SDVIILGHVTEKRDKVYLIHFPRDLYVDIPGRGKDKINAAYAYGGGALLAKTLQNLTH
                     VKIDHAAKIDFTGFQRMTDAVGGVRVYAEEASKESGFTIQKGWNNLNGEQALQFVRER
                     YQLKEGDISRGRRQMAFVKALMVKTLSPGVVLNPVKLTSFINAATDNAVVSKGLTGKF
                     LRSEAIAMRDLRGDDIVFITAPFSGYGTAPNGGAIDILDQPRMESLALALQDDKVEEY
                     LADK"
     gene            2163..3203
                     /locus_tag="PKU75_09700"
     CDS             2163..3203
                     /locus_tag="PKU75_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HPF81193.1"
                     /translation="MTVPAPPPATSPLLARAGAVAGDGVEGGIAIHYGDPLREQRLLV
                     EGLAAVDLSHRDVVTVTGPDRLSWLHSMTTQHLAGLAAWTSRESLVLSPKGHIEHDLH
                     LVDDGETTWITLEPGTGAALVEWLAKMRFMLRVEPELVTERYAVLGEPIGREFAPGDP
                     AGVAAWRDPWPDLVGDTAAYGPVEHPATGRAWRELIVAREDLEAAVGDRPLAGMWAAE
                     ALRVAAWRPRLGFETDHRTIAHEVDWLRTAVHLHKGCYRGQETIARVHNLGRPPRRLV
                     FLHLDGSGHTLPERGAAIELDGRPVGHLTSVARHYEDGPIALGMIKRNTPVDAVLHAG
                     DISAAQTVIVTP"
     gene            3224..4048
                     /locus_tag="PKU75_09705"
     CDS             3224..4048
                     /locus_tag="PKU75_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPF81194.1"
                     /translation="MSTLITVAPTGAEHAKADVPQLPTTLEELVETARSCEAAGAAMI
                     HLHIRDAEHRPTLDVGLLTAAVDAVREATDLIVQLSTGGSVHDPLENRLKVLDAAPDS
                     CSLTCGTTNFGDDVFMNPHGFMSELYVLAQEREVVPEFELFELGHVAALSRLINAHGL
                     PYGGTVHVDFVTGVPGAMPGTPAALMAGVAMLPPEVTSWSATGIGRTHLPILATALAA
                     GGHARVGMEDNIVYAKGQLVQDNSELVARAARLATELQRPPMTTTEARAFLGIKAR"
     gene            complement(4133..4567)
                     /gene="dtd"
                     /locus_tag="PKU75_09710"
     CDS             complement(4133..4567)
                     /gene="dtd"
                     /locus_tag="PKU75_09710"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPF81195.1"
                     /translation="MRAVVQRAHGASVSVAGERISGFDGPGLVVLAGVTHDDGPDQVE
                     WLARKIAQLRILRDERSAQDEGAPVLLISQFTLYADTRKGRRPSWNGAAPGPVAEPLV
                     ADLGRALAAYGLPVHAGVFGADMAVQFTNDGPVTIILDTATP"
     gene            complement(4575..5549)
                     /locus_tag="PKU75_09715"
     CDS             complement(4575..5549)
                     /locus_tag="PKU75_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPF81196.1"
                     /translation="MSTPHAPAVLTEAPVLAHMLRGGFVESVHRASVAVTAADGSIER
                     VLGDGVSPIFPRSANKPLQALAMVRLGLDLPDHLLALACSSHSGLPYHLAGTREILAV
                     ASLDESALQNTPDYPYDAAERDRVIAGGEPKRAVYQNCSGKHAGMVVTCAGQGWPLET
                     YRDVDHPLQVAMADTLAELAGEPVAAMAVDGCGAPVMAVSLGGLARAFGRLAAADPST
                     AEGRVAAAMRAHPEYVGGEGRDVTALIAGTPGLIAKDGAEAVYAVGLADGRGIALKVA
                     DGGQRARPVILAAVLRALGVESAAYETLEDAPVLGHGEPVGAVVAVGV"
     gene            complement(5652..6344)
                     /locus_tag="PKU75_09720"
     CDS             complement(5652..6344)
                     /locus_tag="PKU75_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81197.1"
                     /translation="MAKTTKKFDLRKAVTETTAPVYAYIGMTDLLIERFREATIKAGE
                     TAEKNRAEFAALPTKVQENAAELMEELQNAPVKAREMAEKAQEQYEELVTRGEKLVDR
                     IRNQQSTKDLVAQAKHTVEMTEDAVETARKSFMDVEKSAKATFTTGRKEAAKFVGSVS
                     DTVAQDAKEVAAEAEGAVKRTRTAAKRTTTTAKNRTTKTRAAAETAGTNVRKTATATR
                     KATKKAAEKVGD"
     gene            complement(6392..6874)
                     /locus_tag="PKU75_09725"
     CDS             complement(6392..6874)
                     /locus_tag="PKU75_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF81198.1"
                     /translation="MKTNPLGDLGQTLGEYLREQRQAARLSVRQLSELAGVSNPYLSQ
                     IERGLKKPSAEILNQLAKGLQVSSESLYIRAGLLDAHEGSAPARPETRAVIKSDPRLT
                     SRQKSTLLAVYDSFVDAESTPTPPEIPASEPAQAPRPARKRAAAKAPAEAAAQASHTA
                     "
     gene            6964..7281
                     /locus_tag="PKU75_09730"
     CDS             6964..7281
                     /locus_tag="PKU75_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HPF81199.1"
                     /translation="MFHTVQSWVLLILGAGALGLEAFAVVDALRRRPDAFVAAGKRTK
                     QFWTILLGVAAAIGFVTLFSPLNIFGLLAVVAGAVYLADVRPALDQVQGKGGQRMGPY
                     GPW"
     gene            7289..8146
                     /gene="fdhD"
                     /locus_tag="PKU75_09735"
     CDS             7289..8146
                     /gene="fdhD"
                     /locus_tag="PKU75_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPF81200.1"
                     /translation="MGRVTARLPVTKVRYADPPGARRRADVVAVEEPLEVRAGGQVLT
                     VTMRTPGDDLDLVHGWLLAEGVLRRAEDLRTARYCAGAVETGPDGTPQNTYNVLDVDL
                     APEALAAVPAAQRLGLTSSACGVCGSASIEVLAAKLPGGGLADVDTTLDIATIPELVE
                     GLGAAQRTFAKTGGTHAAALYGAGGELRSAAEDVGRHNAVDKVIGAALRAGAEVPFGG
                     ILLVSSRASFEIVQKAAMAGVPIVIALSAPSSLAVEAARDLGMTLLAFARGESVNVYV
                     GGERLRPPS"
     gene            complement(8103..8705)
                     /locus_tag="PKU75_09740"
     CDS             complement(8103..8705)
                     /locus_tag="PKU75_09740"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPF81201.1"
                     /translation="MDFACLIVAGGRSSRMGEDKITATVGADRVLDRVLAACAPAPVT
                     VAGPPHLAAVLPAGGVLVREDPPFGGPVAGLRAAVAALPQGVPVVAVLAGDQPFVDAA
                     ALAELRAALTAEPGADAAAYVTDDGVLQFLCSVWRAPALRAQLASARDSMRSVYAGAR
                     VVQVPDQRAVSADIDTPEDLATARERVTTAGEDAPHRHTH"
     gene            complement(8744..9541)
                     /locus_tag="PKU75_09745"
     CDS             complement(8744..9541)
                     /locus_tag="PKU75_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF81202.1"
                     /translation="MSRSVVGAVTRGTTNPNRLRRCDRWLAGPQAWRLRRAARPIVVD
                     LGYGASPVTAVELRDRLRRVRPDVEVVGIEIDPARVAAAAPLAGPGLSFRLGGFEIPL
                     DGDARPVVVRAFNVLRQYDEAQVVAYWDLVRSRLAPDGLFVDGTCDEIGRRAAWVALD
                     DAGPQTLTLSWRLAGLERPSQIAERLPKALIHRNVSGEPIHDLLTELDQHWQYAAGLA
                     PYGARQRFVAAAQSLADGGWPLRFPERWRIGEITIDWPAVAPRGSRS"
     gene            9578..10144
                     /locus_tag="PKU75_09750"
     CDS             9578..10144
                     /locus_tag="PKU75_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPF81203.1"
                     /translation="MTPTRELIPSALDELDVEWEPGVSDHEWVVTLPGEKKLKTIVSL
                     LISGETLVTRAFVVRNPDENHGEVYRHLLRRNLRLPGLAYAIDATGDVYVVGRVPVAV
                     VDLGYLDQLLGVVLEAADAPFNELLVLGFLTSMKREWAWRVARGESLRNLEAFRPLLE
                     GSENDPRYAVRDDPGEETEATTTAGEPR"
     gene            10155..10892
                     /locus_tag="PKU75_09755"
     CDS             10155..10892
                     /locus_tag="PKU75_09755"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016663919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HPF81204.1"
                     /translation="MTYTLILLRHGESEWNQKNLFTGWVDVDLTDKGRAEAARGGELM
                     KEAGLLPDVVHTSVLRRAITTANLALDACDRHWIPVHRSWRLNERHYGALQGLDKAAT
                     REKYGDEQFMLWRRSFDTPPPPIEEGSEFDQTGDPRYVGVEVPLTECLKDVIPRMMPY
                     WEDEITDDLKSGQTVLVVAHGNSLRALVKHLDGISDEDIAGLNIPTGMPLVYEIGDDM
                     MPTGPSRYLDPEAAAAAAAAVANQGKQ"
     gene            complement(10976..11170)
                     /locus_tag="PKU75_09760"
     CDS             complement(10976..11170)
                     /locus_tag="PKU75_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81205.1"
                     /translation="MTWSMGDLHGIVDVTADGLVVIDARADADVASLRLVSSDANGNT
                     LADYSAKTAPDAADPFDLHP"
     gene            complement(11167..11538)
                     /locus_tag="PKU75_09765"
     CDS             complement(11167..11538)
                     /locus_tag="PKU75_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HPF81206.1"
                     /translation="MDQHHEAQFREFVEARWQTLLRTAILLTGDQGRAEDLVQQTLVI
                     VHRKWRTIGPGNATAYARAVLANESASWWRRRRYAEVSLSTPAGESAAQVHADPAAPD
                     CRASQAGPPRRSLRSHSTPRP"
     gene            complement(11717..>12235)
                     /gene="phoU"
                     /locus_tag="PKU75_09770"
     CDS             complement(11717..>12235)
                     /gene="phoU"
                     /locus_tag="PKU75_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPF81207.1"
                     /translation="LAIDLLARQAPVATELRQVVTAMRMSSDLERMGDLARHVAKVAR
                     LRYPESAVPAQARAIIMQMSQVAERIVAKAGSVIASHDLKEAASLERDDDAMDELHRQ
                     LFSLLADPAWEHSIPVAVDLTLLGRYYERFADHAVSVARRVTFLVTGEYDTATDRQED
                     EDAAIVGAAQQP"
BASE COUNT         1735 a         4266 c         4391 g         1843 t
ORIGIN      
        1 ggcctgctgg tctacacctt cgcccggctg ctgctcggct agccaccgcg cctcgcggcc
       61 ggcccgcgag cgggcaatgt gcggggccct cagtgcaccc ttactacacc attgctccct
      121 cggtacacca atgcccctcg gtacaccaat gcccctcagt acaccaaggc cccctcagta
      181 caccaaggcc tgcgtgccct ccgtcagggc ctcctcgacg aacgcggcgg cgccgcggat
      241 cgccacgcct ggccgtagcg cctccgcggt gaggtcgcgg cgggcggcgc actgactgca
      301 taccgtcacc tggccggccg cgagcaccgc ctcgagcagc tcggcgggat tcgcggcgtg
      361 cgggagggtg agggcttcgg cccgaccggg cacggcgagc cagctcgcct ccccggtgag
      421 ccacaggctg acctcggcac cgctcgccac ggcggtggcg gcgacggtca gggcctggtt
      481 ggcgcgctcc aacgcctcct ccccgcaggt gagcttgacg agcaggtggg gggctggcga
      541 tgtcatagcg ccagactacg atcgataccg tgttcaccct cgacgagagc attcacgagc
      601 aggtccgccc gctggcctgg ctgatcgggc gttgggaggg cgcgggggtc gtcggctatc
      661 cgacgatgga gtcgcgcaat ttcgggcagg agatcgtcgt cgagcacgac gggcggccct
      721 tcctgcgctg gcagacgcgc agttggctgc tgaacgagga cggcgccatc gaccggccgg
      781 cgtcgaccga gatgggtttt tggcggatgg ccggcgatga ggccgagctg ctgctcgccc
      841 accccacggg catcctcgag ctgtatttcg ggccggtcga gccggcccgg atcagcctga
      901 agaccgatgg ggtgctgcgc agcgcacacg ccaaggagta caacgcggcg acgcggctct
      961 atgggctggt caactccaac ctgatgtggg cgatggacat ggccgcggtc ggcacgccgc
     1021 tgaccagcca cgcctcggcg gagctcaagc gcgtcgaata ggaccacccg tgcgggtgcg
     1081 tcacttgtcg gcgaggtact cctcgacctt gtcgtcctgc agggcgagcg cgaggctctc
     1141 catccggggc tggtcgagga tgtcgatggc gccgccattg ggcgcggtgc cgtagccgga
     1201 gaaaggggcg gtgatgaaga cgatgtcgtc gccgcgcaga tcgcgcatcg ctatcgcctc
     1261 ggagcgcagg aacttgccgg tcaggccctt cgagacgacg gcattgtccg tcgcggcgtt
     1321 gatgaacgag gtgagcttga ccggattcag cacgacgccg gggctgaggg tcttgaccat
     1381 cagcgccttg acgaaagcca tctggcgccg gccgcgggag atgtcgccct ccttgagctg
     1441 gtagcgctcg cggacgaact gcagggcctg ctcgccgttg aggttgttcc agcccttctg
     1501 gatggtgaag ccggactcct tgctcgcctc ctcggcatac acccggaccc cgccgacggc
     1561 gtcggtcatc cgctggaacc cggtgaagtc gatcttggcg gcatggtcga tcttgacgtg
     1621 ggtgaggttc tgcagcgtct tggcaagcag ggcgccgcct ccgtaggcgt aggccgcgtt
     1681 gatcttgtcc ttgccccgcc ccgggatgtc gacgtagagg tcgcggggga agtggatcag
     1741 gtagaccttg tccctcttct ccgtgacgtg gccgaggatg atgacgtcgc tgcggccggc
     1801 gtcgccggga cgggcgtcgg agccgatgat cagatagttc gtgcccggtg actcgaccag
     1861 gggttcgctc tccccgtccg cggggtcggt gcccgggttg tcgttgccct gggccgcgtc
     1921 gggcggcagg agggtgtcgt cgacggcgat atgggagatc cggttgttga gatagaggac
     1981 gaacccaccc atggcgccca cggcgagcag tgccaggccg aggagaaccg cgaggatgcg
     2041 ccgccctcgg cgtctcgtgc gcggcgggga tgcctgatcg gggaggctgt gcgacaccgc
     2101 tgcagtgtac gagcagacgc tgagccgggg taggcccgct ttcgacctgg gatcatggac
     2161 gtatgaccgt gcctgccccg cctcctgcca cctcaccgct gctggcccgc gccggggcgg
     2221 tcgccggcga cggggtcgag ggtggcatcg ccattcatta cggcgatccg ttgcgggagc
     2281 agcggctgct ggtcgagggc ctcgcggccg tcgatctgtc ccaccgggat gtcgtgaccg
     2341 tcaccggccc ggaccggctc tcctggctgc attcgatgac gacacaacac ctggccggcc
     2401 tggcggcctg gacctctcgg gagtccctgg tgctctcgcc gaaggggcat atcgagcacg
     2461 atctgcacct ggtcgacgac ggcgagacga cgtggatcac gctcgagccc ggcaccggcg
     2521 cggccctggt cgagtggctg gcgaagatgc ggttcatgct gcgggtcgaa cccgagctgg
     2581 tcaccgagcg gtatgccgtg ctcggcgagc ccatcgggcg cgagttcgcc ccgggagacc
     2641 cggccggcgt ggcggcctgg cgcgatccgt ggccggacct ggtgggggac accgcggcat
     2701 acggtcccgt ggagcacccg gcgaccggcc gtgcgtggcg cgagctgatc gtggcccgcg
     2761 aggatctgga ggcggcggtc ggggaccggc cgctggcggg gatgtgggcg gccgaggcgc
     2821 tgcgggtggc ggcgtggcgg ccgcggctcg gcttcgagac cgaccaccgc accatcgcgc
     2881 acgaggtgga ctggctgcgc accgcggtgc acctgcacaa ggggtgttat cggggtcagg
     2941 agacgatcgc ccgggtgcac aacctgggcc gcccgccgcg gcggctggtc ttcctccacc
     3001 tcgacggctc cgggcacacg ctgcccgagc gcggggcggc gatcgagctg gacggccggc
     3061 cggtggggca cctgaccagc gtcgcccggc actacgagga cgggccgatc gccctcggga
     3121 tgatcaagcg caacaccccg gtcgacgccg tcctgcatgc cggcgacatc agcgccgccc
     3181 agaccgtgat cgtcaccccc tgacgacccg ccatgatggg ggcatgagca ccctcatcac
     3241 cgtggcgccc accggcgccg agcatgccaa ggccgacgtc ccccagctgc ccaccaccct
     3301 cgaggagctg gtcgagacgg cgcgctcgtg cgaggccgcg ggcgcggcca tgatccacct
     3361 gcacatccgc gacgccgagc accggccgac gcttgacgtc gggctgctca ccgcggcggt
     3421 cgacgcggtg cgggaggcga cggacctgat cgtgcagctg tcgacgggcg gctcggtgca
     3481 tgacccgctg gagaaccggc tcaaggtcct cgacgccgct ccggactcgt gcagtttgac
     3541 ttgtgggaca acgaatttcg gcgatgacgt gttcatgaac ccgcatggct tcatgagtga
     3601 gctctatgtc ctggcgcagg agcgtgaggt ggtgccggag ttcgagctct tcgaactggg
     3661 gcatgtggcg gcgctgtcgc ggctgatcaa cgcgcacggg ctgccctatg gcggcacggt
     3721 gcacgtcgac ttcgtgaccg gcgtccccgg cgcgatgccg ggcacgccgg cggcgctgat
     3781 ggcgggggtc gcgatgctgc cgccggaggt gacgagctgg tcggccaccg gcatcggccg
     3841 cactcacctg ccgatcctcg cgacggcact cgccgcgggc ggccacgccc gggtcgggat
     3901 ggaggacaat atcgtctacg ccaaaggtca actcgtgcaa gacaattcgg agctcgtcgc
     3961 ccgcgccgcc cgcctcgcga ccgaactcca gcgccccccg atgaccacca ccgaggcccg
     4021 cgccttcctc ggcatcaagg cgcgctgagg ccccggctcc ccacgtaacc tctgctacta
     4081 gcagaggtta cgtgggcacg ccgaagtgcc ttgagcggca gggagatccg gctcaggggg
     4141 tggccgtgtc gaggatgatg gtgacggggc cgtcgttggt gaactgcacg gccatgtcgg
     4201 caccgaagac gcccgcgtgc accggcaggc cgtatgccgc gagcgcgcgc ccgaggtcag
     4261 cgacgagggg ctcggcgacc gggccggggg cggcgccgtt ccacgacggg cgccggccct
     4321 tgcgggtgtc ggcatagagc gtgaactggc tgatgagcag caccggcgca ccttcgtcct
     4381 gcgccgagcg ctcgtctcgc aggatccgca gctgggcgat cttgcgcgcc agccactcca
     4441 cctgatccgg tccgtcgtcg tgggtgaccc cggcaagcac cacgagcccc ggcccgtcga
     4501 agcccgagat gcgctctccc gcaaccgaaa cgctcgcgcc gtgcgcgcgc tggacgacag
     4561 ctctcacggc agcgtcacac gccgacggcg acgaccgcgc cgaccggctc accatgcccg
     4621 agcacgggcg cgtcctccag cgtttcgtat gccgcggact ccaccccgag cgcacgcagc
     4681 accgccgcga ggatgaccgg ccgcgcgcgc tgcccgccgt cggcgacctt cagggcgatg
     4741 ccgcggccgt cggccagccc cacggcatac accgcctcgg cgccgtcctt ggcgatcagg
     4801 cccggtgtcc ccgcgatcag cgcggtgaca tcgcggccct cgccgccgac atactccggg
     4861 tgcgcgcgca tggcggccgc tacccgcccc tcggcggtgc tcggatccgc cgcggccagc
     4921 cggccgaagg cccgcgccag gccgccgagc gaaacggcca tgaccggcgc gccgcagccg
     4981 tcgacggcca tcgccgcgac gggctcgccg gcaagctcgg caagcgtgtc ggccatcgcc
     5041 acctgcaggg gatggtcgac gtcgcggtag gtctccagcg gccagccctg gccggcgcag
     5101 gtgacgacca tcccggcgtg cttgcccgaa cagttctggt agacagcgcg tttcggctcg
     5161 ccgccggcga tcacccggtc ccgctcggcg gcatcgtagg gatagtcggg agtgttctgc
     5221 agggcgctct cgtccaggct cgcgacggcg aggatctccc gggtgccggc caggtgatag
     5281 ggcaggcccg agtggctcga gcaggccagc gccagcaggt ggtcgggcag gtcgagcccg
     5341 agacggacca tcgccagggc ctgcagcggc ttgttggccg accgcgggaa gatcgggctc
     5401 acgccatcgc cgagcacccg ctcgatcgag ccgtccgcgg ccgtgacggc gacgctcgcg
     5461 cggtgcacgg attcgacgaa gccgccccgc agcatgtggg ccagcacggg cgcttcggtc
     5521 aggacggcgg gggcgtgggg ggtgctcacg cgtggcgaga gtagcccacc gcggcatacc
     5581 cgggcacggc gaagggggcc cggggactac tcctccggac ccccttcacg atggggtcag
     5641 ccccgtgggg ctcagtcgcc gaccttctcg gcggccttct tggtggcctt gcgggtggcg
     5701 gtggccgtct tgcggacgtt ggtgccggcg gtctcggcgg cggcgcgcgt cttggtggtg
     5761 cggttcttgg ccgtggtggt ggtgcgcttg gccgcggtgc gggtgcgctt gacggcgccc
     5821 tcggcctcgg cggcgacctc cttggcgtcc tgggccacgg tgtcgctgac ggagccgacg
     5881 aacttcgcgg cctccttgcg gccggtggtg aaggtggcct tggcggactt ctcgacgtcc
     5941 atgaaggact tgcgggccgt ctcgacggcg tcctcggtca tctcgacggt gtgcttggcc
     6001 tgggcgacga ggtccttggt ggactgctgg ttgcggatgc ggtcgacgag cttctcgcca
     6061 cgggtgacga gctcttcgta ctgctcctgc gccttctcgg ccatctcgcg agccttgacg
     6121 ggcgcgttct gcagctcctc catgagctcg gcggcgttct cctgcacctt ggtgggcagg
     6181 gcagcgaact cggcgcggtt cttctcggcg gtctcgccgg ccttgatggt ggcctcgcgg
     6241 aaccgctcga tgagcaggtc ggtcatgccg atgtaggcgt agaccggcgc ggtggtttcg
     6301 gtgaccgcct tgcggaggtc gaacttcttg gtggtcttgg ccacgatctt ccttctttcg
     6361 gttgccggct cgcgaaagcc ggggcgtggg gtcaggcggt gtgcgacgcc tgggcggcgg
     6421 cctcggcggg agccttggcg gccgcacgct tccgagccgg acgcggggcc tgcgccggct
     6481 cggaagcggg gatctcgggg ggagtggggg tggactcggc gtcgacgaag ctgtcgtaga
     6541 cggcgagcag ggtgctcttc tggcggctgg tcagtcgcgg gtcggacttg atgaccgcac
     6601 gggtttccgg acgagcgggg gcggagccct cgtgggcgtc gagaaggccg gcgcggatgt
     6661 acaaggactc cgaggagacc tgcagaccct tggcgagctg gttgaggatc tcggcgctcg
     6721 gcttcttcag gccccgctcg atctgcgaca gataggggtt ggagacgccg gcgagctccg
     6781 agagctggcg gacggacagc cgcgcggcct ggcgctgctc gcgcaggtac tcgcccaggg
     6841 tttggcccag gtcgccgagc ggattcgtct tcacccctcc attgtgcttg ctgtagttag
     6901 caaaatgcaa actcgggtgt cgtgatttag gccacgtgca ggtcggtcgc ttatcgttgg
     6961 cgcatgttcc acaccgtcca gtcctgggtg ctgctgatcc tcggtgccgg cgccctcggc
     7021 ctggaggcct tcgccgtcgt cgacgcgctg cgccgccgcc ccgacgcctt cgtcgccgcg
     7081 gggaagcgga ccaagcagtt ctggacgatc ctgctcggcg tcgccgccgc gatcgggttc
     7141 gtcacgctgt tcagcccgct caacatcttc ggcctgctcg ccgtcgtcgc cggcgccgtc
     7201 tatctcgccg atgtccgccc ggccctcgat caggtgcagg gcaagggcgg gcagcggatg
     7261 gggccctacg gcccttggtg acgagctgat gggccgggtc accgctcgcc tgccggtcac
     7321 caaggtgcgg tatgccgacc cccccggtgc gcgtcggcgc gcggacgtcg tcgcggtgga
     7381 ggagccgctc gaggtccggg cgggcgggca ggtgctcacc gtcaccatgc gcacgcccgg
     7441 ggacgatctc gacctggtcc acggctggct gctcgccgaa ggcgtgctgc gccgggccga
     7501 ggacctgcgc accgcgcgct attgcgccgg cgccgtggag accggcccgg acggcacgcc
     7561 gcagaacacc tacaacgtcc tcgacgtcga cctggccccg gaggcgctcg ccgccgtgcc
     7621 cgccgcgcag cgcctcgggc tgacgtccag tgcgtgtggg gtctgcggct cagcctccat
     7681 cgaggtgctc gccgccaagc tgcccggcgg cggcctcgcg gacgtcgaca ccacgctcga
     7741 tatcgccacc atcccggagc tggtcgaggg gttgggcgcc gcgcagcgca ccttcgccaa
     7801 gaccggcggc acccacgcgg cggcgctcta tggcgccggt ggcgaattgc gttctgcggc
     7861 agaggatgtc ggacgccaca acgccgtcga caaggtcatc ggcgcggccc tgcgcgccgg
     7921 cgccgaggtg ccgttcggcg gcatccttct cgtctcctcc cgcgcctcct tcgagatcgt
     7981 ccagaaggcc gcgatggccg gcgtgccgat cgtcattgcg ctgtcggcac cgtcttcgct
     8041 ggccgtcgag gcggcccggg atctcgggat gacgttgctt gccttcgcgc gcggcgagag
     8101 cgtcaatgtg tatgtcggtg gggagcgtct tcgcccgccg tcgtgacccg ctcgcgcgcc
     8161 gtggcgagat cctccggcgt gtcgatatcg gcgctcaccg cccgctggtc ggggacctgg
     8221 acgacccgcg cgccggcata caccgagcgc atcgaatccc gggcggacgc gagctgagcg
     8281 cgcagggcgg gcgcgcgcca tacggagcaa aggaactgca gcacaccgtc atcggtgaca
     8341 taggccgccg cgtccgcgcc gggctccgcg gtcagggcag cgcgcaactc ggcgagcgcg
     8401 gccgcgtcga cgaagggctg gtcgcccgcg agcaccgcca ccaccggcac gccctggggc
     8461 agggcggcga cggcagcgcg caggccggcc acggggccgc cgaacggcgg gtcctcacgg
     8521 accagcacgc caccggcggg cagcacggcc gccagatgcg gcggcccggc gacggtcacc
     8581 ggcgccgggg cacacgccgc gagcacccgg tcgagcactc ggtcggcgcc gacggtcgcg
     8641 gtgatcttgt cctcgcccat ccggctggag cgcccgccgg cgacgatcag gcacgcgaaa
     8701 tccattgccg cagaatcgat atccgtcgcg tcaggtgttt ccttcacgac cggctcccgc
     8761 gcggcgccac cgcgggccag tcgatcgtga tctccccgat ccgccagcgc tccgggaaac
     8821 gcaagggcca cccgccgtcg gcgagcgact gcgccgcggc gacgaaacgc tgccgcgcgc
     8881 catagggagc cagcccggcg gcatactgcc aatgctggtc gagctcggtg agcaggtcat
     8941 ggatcggctc gcccgagaca ttgcggtgga tgagggcttt cggcaggcgc tcggcgatct
     9001 gcgacggccg ctcgagaccg gccagccgcc aggacagcgt cagggtttgg ggccctgcat
     9061 catcgagcgc gacccacgcc gcccggcgcc cgatctcatc gcacgtgcca tcgacgaaaa
     9121 gcccgtccgg cgccagccgg gagcgcacca agtcccaata tgcgacgacc tgagcctcgt
     9181 catactgccg cagcacgttg aacgcccgga cgaccacggg acgggcatcc ccgtcaaggg
     9241 gaatctcgaa gcctcccaag cgaaacgaca gtcccggccc ggcgagcggg gcggcggccg
     9301 ccacccgggc cgggtcgatc tcgatgccca ccacctcgac atccgggcgg acccggcgca
     9361 gccggtcccg cagctccacg gctgtcacgg gtgaggcgcc gtagccgaga tcgacgacga
     9421 tcggccgcgc cgcccgccgc agccgccacg cctgcggccc ggcgagccac cggtcgcacc
     9481 ggcgcagccg gttggggttc gtcgtgcccc gcgtgacggc gccgacgacg gaacgactca
     9541 cgccggtcag catacgaatc gtgggagggt gaagggcatg acgccgaccc gtgagctcat
     9601 cccttcggcc ctcgacgaac tcgacgtgga atgggagccg ggggtgtccg accatgaatg
     9661 ggtcgtcacc ctacccgggg agaagaagct caaaacgatt gtctcgctgt tgatctcggg
     9721 cgaaacgctg gtgacgcgcg ccttcgtcgt ccgcaacccc gacgaaaacc acggcgaggt
     9781 ctaccggcat ctgctgcgcc gcaatctgcg gctgcccggt ctcgcctacg ccatcgacgc
     9841 cacgggcgat gtctatgtcg tcggacgcgt gcccgtcgcc gtggtcgacc tggggtacct
     9901 cgaccagctc ctcggggtcg ttctcgaggc cgccgacgca cccttcaacg aactgctcgt
     9961 cctcggcttc ctcacctcga tgaagaggga gtgggcctgg cgggtggcgc ggggggagtc
    10021 gctgcgcaat ctcgaggcct tccggccgct gctggagggc agcgagaacg atccacggta
    10081 tgccgtgcgc gacgaccccg gcgaggagac cgaggccaca acgacagcgg gggagcctcg
    10141 atagggtggc gggtatgacg tacacgctca tcctgctgcg ccacggcgaa tccgagtgga
    10201 accagaagaa cctcttcacc ggctgggtcg acgtcgacct gaccgacaag ggccgcgccg
    10261 aggccgcgcg cggcggcgag ttgatgaagg aggccggcct cctgcccgac gtcgtgcaca
    10321 cctcggtgct gcgccgggcc atcacgacgg ccaacctcgc cctcgacgcg tgcgaccggc
    10381 actggatccc ggtgcaccgc agctggcggc tcaacgagcg tcactacggc gcgctgcaag
    10441 gactggacaa ggcggccaca cgcgagaagt acggcgacga gcagttcatg ctgtggcggc
    10501 gcagcttcga caccccgcca cccccgatcg aggagggcag cgagttcgac cagaccggtg
    10561 acccgcgcta tgtcggcgtg gaggtgcccc tgaccgagtg cctcaaggac gtcatcccgc
    10621 ggatgatgcc ctactgggag gacgagatca ccgacgacct gaagtccggt cagaccgtcc
    10681 tcgtcgtcgc gcacggcaac tccctgcgcg cgctcgtcaa gcacctcgac ggcatctccg
    10741 acgaggacat cgccggcctg aacatcccca ccggcatgcc gctcgtctac gagatcggcg
    10801 acgacatgat gcccaccggc ccgagccgct acctcgaccc cgaagccgcc gccgcggccg
    10861 ccgcggccgt cgccaaccag ggcaagcagt aacccacggg ggctccgccc ccgtgcaccc
    10921 ccgacgacaa ccccaatgac agcccaccgg gaagtggcgc tctgcccgga agcggtcagg
    10981 ggtgcaggtc gaaggggtcc gcggcatcgg gggcggtctt ggcggagtag tccgcgaggg
    11041 tgttgccgtt cgcgtccgaa ctgaccaggc gcaaggaggc gacgtcggcg tccgctcggg
    11101 cgtcgatgac gacgaggccg tcggccgtga cgtccacgat gccgtgcagg tcgcccatcg
    11161 accatgtcac ggtcttggcg tcgagtgcga ccgcaaggat cttcgtggcg gccccgcctg
    11221 actcgccctg cagtccggtg ccgcggggtc ggcatgcacc tgagccgcgg attcgcccgc
    11281 cggcgtgctg agggagacct cggcataccg cctgcggcgc caccacgagg cggactcgtt
    11341 ggcgagcacc gcccgggcgt atgccgtggc gttgccgggt ccgatggtgc gccactttcg
    11401 gtgcacgatg accagcgtct gttgcaccag atcctcggca cggccctgat cgccggtgag
    11461 caggatcgcg gtgcgcagca acgtctgcca gcgcgcctcg acgaactcac ggaactgcgc
    11521 ctcgtggtgc tgatccatcg cggcctcccg gtgagatctt catctacgag aggtgccggg
    11581 gccggcagca ggttgacacg ccgtcggact ttctccacat ccgggaagga gaaaacaacg
    11641 tggccccggc cggatctctc cgaccggggc tgctgtcccc cgtttcccct ccaaccccgt
    11701 cgccggggtg gctcggttac ggctgctgcg ccgcgccgac gatggccgcg tcctcgtcct
    11761 cttggcggtc ggtggccgtg tcgtactcac ccgtcacgag gaaggtcacc cgccgggcaa
    11821 ccgagacggc gtggtcggcg aaccgctcgt aatagcggcc gagcagcgtc aggtcgacgg
    11881 cgaccgggat gctgtgctcc catgccgggt ccgccaacag gctgaacaac tgccggtgca
    11941 attcgtccat cgcgtcgtcg tcgcgctcga ggctggcggc ctccttgagg tcgtgcgagg
    12001 cgatgaccga gccggccttg gcgacgatac gttcggcgac ctggctcatc tgcatgatga
    12061 tggcgcgggc ttgagcgggc acggcggact cgggatagcg cagccgcgcc accttggcca
    12121 cgtgccgggc cagatcgccc atccgctcca ggtcggagct catccgcatg gccgtgacga
    12181 cctgacgcaa ctcggtcgcc accggcgcct gccgcgccag cagatcgatc gcgag
//
LOCUS       DAOPJG010000096        12212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5458_length_12212_cov_7.035453, whole genome shotgun sequence.
ACCESSION   DAOPJG010000096 DAOPJG010000000
VERSION     DAOPJG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12212
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5458_length_12212_cov_7.035453"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKU75_09775"
     CDS             <1..232
                     /locus_tag="PKU75_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPF81208.1"
                     /translation="AKTTFGRIGVKVWIYKGDMTEKEFAAQQVNAPRGGARGPRRDRD
                     DRPARARRDAAPATTETAEASAPAAEAVTEGA"
     gene            240..659
                     /gene="rplP"
                     /locus_tag="PKU75_09780"
     CDS             240..659
                     /gene="rplP"
                     /locus_tag="PKU75_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPF81209.1"
                     /translation="MLIPRRVKHRKQHHPGRSGMSKGGTSLAFGEYGIQALEPAYVTN
                     RQIESARIAMTRYIKRGGKVWINIYPDRPLTKKPAETRMGSGKGSPEWWIANVKPGRV
                     LFELSGVPEDVAREAMRLAMHKLPMKTRFVKREGGDI"
     gene            659..892
                     /gene="rpmC"
                     /locus_tag="PKU75_09785"
     CDS             659..892
                     /gene="rpmC"
                     /locus_tag="PKU75_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPF81210.1"
                     /translation="MAIGSKDLTPTELRGLADDRLVEELRKAKEELFNLRFQSATGQL
                     ESHGRLRAVRKDIARIYTEMRERELGIGTGPEA"
     gene            898..1185
                     /gene="rpsQ"
                     /locus_tag="PKU75_09790"
     CDS             898..1185
                     /gene="rpsQ"
                     /locus_tag="PKU75_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPF81211.1"
                     /translation="MSENTVEEKSSVERNYRKTRQGMVVSDKMDKTVVVEVEDRVKHA
                     LYGKVMRKSHKVKAHDEQNAAGVGDRVLIMETRPLSASKRWRVVEILEKAK"
     gene            1182..1550
                     /gene="rplN"
                     /locus_tag="PKU75_09795"
     CDS             1182..1550
                     /gene="rplN"
                     /locus_tag="PKU75_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPF81212.1"
                     /translation="MIQQESRLKVADNTGAKEILCIRVLGGSGRRYAGIGDTIVATVK
                     DAIPGGNVKKGDVVKAVVVRTTKERRRPDGSYIRFDENAAVILKNDGDPRGTRIFGPV
                     GRELRDKRFMKIISLAPEVL"
     gene            1556..1891
                     /gene="rplX"
                     /locus_tag="PKU75_09800"
     CDS             1556..1891
                     /gene="rplX"
                     /locus_tag="PKU75_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPF81213.1"
                     /translation="MGTMKIKKGDTVQVLTGKDKAKQGKVIAVDTENQRVTVEGVNRV
                     TRHVKPGTRGTGGLVVQEAPIHVSNVAVIDPTDKKPTRVKTREEQVERDGRTKTTRVR
                     VSARSGKDI"
     gene            1891..2460
                     /gene="rplE"
                     /locus_tag="PKU75_09805"
     CDS             1891..2460
                     /gene="rplE"
                     /locus_tag="PKU75_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPF81214.1"
                     /translation="MTETAAPAAKPRLKTRYQDEIKSALQDQFNFGNPMQVPGVVKVV
                     VNMGVGDAAKDSKLIEGAIKDLTAITGQKPQITKARKSIAQFKLREGMPIGAHTTLRG
                     DRMWEFLDRLVTVALPRIRDFRGLSPKQFDGRGNYTFGLTEQSMFHEIDQDKIDRVRG
                     MDITVVTTATNDDEGRALLKALGFPFKEN"
     gene            2462..2647
                     /locus_tag="PKU75_09810"
     CDS             2462..2647
                     /locus_tag="PKU75_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPF81215.1"
                     /translation="MAKTALKNKAAKAPKFKVRGYTRCQRCGRAHSVYRKFGLCRICL
                     REMAHRGELPGVTKSSW"
     gene            2850..3248
                     /gene="rpsH"
                     /locus_tag="PKU75_09815"
     CDS             2850..3248
                     /gene="rpsH"
                     /locus_tag="PKU75_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPF81216.1"
                     /translation="MTMTDPIADMLTRVRNANSAHHDEVSMPFSKLKSHIAEILQAEG
                     YIASWAVEDAEVGKTLTINLKYGPNRERSIAGIRRVSKPGLRVYAKSTNLPRVLGGLG
                     IAIISTSSGLLTDKQAASKGVGGEVLAYIW"
     gene            3261..3800
                     /gene="rplF"
                     /locus_tag="PKU75_09820"
     CDS             3261..3800
                     /gene="rplF"
                     /locus_tag="PKU75_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPF81217.1"
                     /translation="MSRIGRMPVTVPANVTVTIDGQQVTVKGPKGELTHTVAEPITVA
                     QGEGNVEVTRPNDERTARSLHGLTRTLINNMVLGVTDGYEKKMEIYGTGYRVMARGSN
                     LEFALGYSHPITVEPPAGITFAVENQTRFSVQGIDKQLVGEVAANIRKLRKPDPYKGK
                     GVRYAGEQIRRKVGKAGKK"
     gene            3803..4177
                     /gene="rplR"
                     /locus_tag="PKU75_09825"
     CDS             3803..4177
                     /gene="rplR"
                     /locus_tag="PKU75_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPF81218.1"
                     /translation="MAMIVKRGKSKAAARGRRHDRIRKHVAGTTERPRLVVSRSSRHL
                     FVQVVDDTVGKTLASASTMEADLRAFDGDKTAKAKKVGELLAERAKAAGVESVVFDRG
                     GNKYHGRVAAIADGARQGGLSL"
     gene            4209..4865
                     /gene="rpsE"
                     /locus_tag="PKU75_09830"
     CDS             4209..4865
                     /gene="rpsE"
                     /locus_tag="PKU75_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPF81219.1"
                     /translation="MPGPQRRGTGAADRGGDRPERGERRGRDGDRRDNRRGDAAEKSQ
                     YLERVVTINRVAKVVKGGRRFSFTALVVVGDGDGTVGVGYGKAREVPAAIAKGVEEAK
                     KAFFKVPRIAGTIPHPVQGEAAAGVVLLRPAAPGTGVIAGGPVRAVLECAGVHDVLSK
                     SLGSDNSINIVHATVAALKDLERPEAVAARRGLPLDEVAPAAMLRAQAAATAGAKAGA
                     "
     gene            4865..5047
                     /gene="rpmD"
                     /locus_tag="PKU75_09835"
     CDS             4865..5047
                     /gene="rpmD"
                     /locus_tag="PKU75_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPF81220.1"
                     /translation="MARLKVTQLKGIVGEKHSARETLRTLGLKRIGDVVVKEDRPEIR
                     GMVRTVRHLVTVEEVE"
     gene            5047..5529
                     /gene="rplO"
                     /locus_tag="PKU75_09840"
     CDS             5047..5529
                     /gene="rplO"
                     /locus_tag="PKU75_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPF81221.1"
                     /translation="MADKAKDTAATAGTHALKVHHLRPAPGAKTAKTRVGRGEGSKGK
                     TAGRGTKGTKARYQVPAHFEGGSTAIHMRLPKLRGFKNPFRTTYQVVNLDKISALYPD
                     GGAVTVEDLVAKGAVRAGEPVKVLGTGEITVAVQVTADAFSASAKEKIEAAGGSTATA
                     "
     gene            5738..7045
                     /gene="secY"
                     /locus_tag="PKU75_09845"
     CDS             5738..7045
                     /gene="secY"
                     /locus_tag="PKU75_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPF81222.1"
                     /translation="MLSAFGRAFKTPDLRKKLLFTLFIIAIYRLGAHVPTPGVSYPLV
                     QQCIQNAPTGGGFLGLANLFSGGALLQLSIFALGIMPYITASIIVQLLTVVIPRFETL
                     KKEGHAGQQKMTQYTRYLTIGLAILQSATLVTFAQNPSSLMQGCERVLVDDKISTILI
                     MVLTMTAGTGMIMWLGELLTDKGVGNGMSILIFTSIVASFPGSLWLIRQQNGWGIFLA
                     IIAMGFLIIALVVFVEQSQRRIPVQYAKRMVGRQMYGGTSTYIPIKVNMANVIPVIFA
                     ASLMALPMMIAQFNTNPTGASPGWVTWINNTLVNGDHPIYMILYTLLILFFTFFYVSI
                     TFNPEEVADNMKRYGGFIPGIRAGKPTAEYLQYVLTRITVVGAIYLALISLIPLIAIA
                     VINANQNFPFGGTSILIMVGVGLETVKQIEAQLHQRHYEGFLR"
     gene            7090..7662
                     /locus_tag="PKU75_09850"
     CDS             7090..7662
                     /locus_tag="PKU75_09850"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPF81223.1"
                     /translation="MRLIIFGPPGAGKGTQASRIAARYGIPAISTGDIFRANIKNETP
                     LGLQVKEILASGGYVSDEITNAIVADRLAQEDCAPGFLLDGYPRTSDQVYALDAMLAE
                     RGGELDAVLELTVDEDEVVDRLLKRAETEGRVDDTEEVIRERLAIYRRETAPLAAIYL
                     ERGLLVQVDGLGEIDEVSERIGVALDERQH"
     gene            7690..8529
                     /gene="map"
                     /locus_tag="PKU75_09855"
     CDS             7690..8529
                     /gene="map"
                     /locus_tag="PKU75_09855"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPF81224.1"
                     /translation="MMFGRERLETKTRDQVRAMRAPGLVVGQCLAMLRDAAQPGVTTL
                     ELDTLAERFIRDHGGRPSFAEVPGYRHTLCTSVNEEIVHGIPRETRVLAAGDLLSIDC
                     GAIVDDWHGDAAISLIVGGREAARPEDLQLLDATEESLWAGIAAMRVGRDLFEVGAAI
                     EKSIVASGKEHDRVFGIVEDYVGHGIGREMHMSPNVPNYAVRGKGPVVKNAATVAIEP
                     MITLGEEANETLADQWTVVTRDRMRACHWEHTVACHDGGIWVLTALDGGAARLGTAYA
                     PLD"
     gene            8785..9792
                     /locus_tag="PKU75_09860"
     CDS             8785..9792
                     /locus_tag="PKU75_09860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HPF81225.1"
                     /translation="MRFDTTYAGLPAEVVGVVEAAEAGGYDGLTIAELQHDPFVTATL
                     ACAQESQLRIGTSVAIAFARSPMTVAQTAWDLQRLSGGRFALGLGSQIEPHITRRFSM
                     PWSKPAARMREYIAAVNAIWDAWQDGTRLNFRGDFYTHTLMTPMFNPGPLRVSRPRIL
                     LAAVGPLMITVAAEAADGLIVHPFTTLGYLTDVLEPLVVDKLGERDRSDFELIGQVMV
                     ASGRDQSQIAASVAAVKAQLAFYGSTPAYRPVLEHHGLGELGDELNRLSKRGEWTAMG
                     ERIDDATFALFAVRGTPQECGADLVRRFGATYDRLSLTLMGASDAELAAGVVGAARAS
                     G"
     gene            9919..10284
                     /gene="trxA"
                     /locus_tag="PKU75_09865"
     CDS             9919..10284
                     /gene="trxA"
                     /locus_tag="PKU75_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPF81226.1"
                     /translation="MATRNLTATDIQSTVTDNDIVLVDFWASWCGPCRQFAPVFEKAS
                     EEHADIIFGKVDTEAEQALAGQLNITSIPTLMAFREGILVFNQAGALPAAGLNQVIEA
                     VKGLDMEQVRSQLVPSPES"
     gene            10382..10516
                     /locus_tag="PKU75_09870"
     CDS             10382..10516
                     /locus_tag="PKU75_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81227.1"
                     /translation="MSRAEVYRWNWRTGLVTLVSTRVTGAEGVYLHSTGQIERTPAAA
                     "
     gene            10704..10925
                     /gene="infA"
                     /locus_tag="PKU75_09875"
     CDS             10704..10925
                     /gene="infA"
                     /locus_tag="PKU75_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPF81228.1"
                     /translation="MAKKDGVIEVEGTIVEALPNAMFRVELSNGHKVLAHISGKMRQH
                     YIRILPEDRVVVELSPYDLTRGRIVFRYR"
     gene            10988..11101
                     /gene="rpmJ"
                     /locus_tag="PKU75_09880"
     CDS             10988..11101
                     /gene="rpmJ"
                     /locus_tag="PKU75_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPF81229.1"
                     /translation="MKVQPSVKKICDKCKVIRRHGRVMVICDNQRHKQRQG"
     gene            11300..11677
                     /gene="rpsM"
                     /locus_tag="PKU75_09885"
     CDS             11300..11677
                     /gene="rpsM"
                     /locus_tag="PKU75_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPF81230.1"
                     /translation="MARLVGVDLPRDKRVEVALTYIFGVGRTTSQKALEATGVDPSIR
                     VKDLVDDQLVALRDYLEANVKIEGDLRREVASDIRRKVEIGSYEGLRHRRGLPVRGQR
                     TKTNARTRKGPKRTVAGKKKAGK"
     gene            11677..12072
                     /gene="rpsK"
                     /locus_tag="PKU75_09890"
     CDS             11677..12072
                     /gene="rpsK"
                     /locus_tag="PKU75_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPF81231.1"
                     /translation="MPPKARATKVRRKVKKNVAVGQAHIKSTFNNTIISITDPTGAVI
                     SWASAGQVGFKGSRKSTPYAAQMAAEACARRAMEHGMRKVDVFVKGPGSGRETAIRSL
                     TAAGLEVGAISDVTPSPHNGVRPPKRRRV"
     gene            12102..>12212
                     /locus_tag="PKU75_09895"
     CDS             12102..>12212
                     /locus_tag="PKU75_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPF81232.1"
                     /translation="MARYTGPTTKKSRRYKLDLVGGDKNFDLRPFPPGQHG"
BASE COUNT         2212 a         4216 c         3956 g         1828 t
ORIGIN      
        1 ggccaagacc accttcggcc gcatcggcgt gaaggtgtgg atctacaagg gcgacatgac
       61 tgagaaggag ttcgctgcgc agcaggtcaa cgccccgcgc ggcggcgccc gcggtccgcg
      121 ccgtgaccgt gacgaccgtc ccgcgcgcgc ccgccgcgac gcggcgccgg ccaccaccga
      181 gaccgccgag gcgagcgcac ccgccgccga ggctgttacc gaaggagcgt gagcgcgcca
      241 tgttgattcc gcgtcgcgtc aagcaccgca agcagcacca ccccggtcgc tccggcatgt
      301 ccaagggcgg cacctcgctc gccttcggtg aatacggcat ccaggccctc gagcccgcct
      361 acgtcaccaa ccggcagatc gagtccgctc gtatcgccat gacccgctac atcaagcgtg
      421 gcggaaaggt ctggatcaac atctacccgg accgtccgct gaccaagaag cccgccgaga
      481 cccgcatggg ttccggtaag ggctcgcccg agtggtggat cgccaacgtc aagccgggcc
      541 gcgtgctatt cgaactctcc ggtgtcccgg aggacgtcgc tcgcgaggcc atgcgcctgg
      601 cgatgcacaa gctgcccatg aagacccgct tcgtcaagcg tgaaggtggt gacatctgat
      661 ggctatcggt agcaaggacc tgacccccac cgagctgcgc ggcctcgccg acgaccgcct
      721 cgtcgaagag ctgcgcaagg ccaaggagga gctgttcaac ctccgtttcc agtcggccac
      781 cggtcagctc gaaagccatg gccgcctgcg cgccgtccgc aaggacatcg cgcggatcta
      841 caccgagatg cgtgagcgcg agctcggcat cggcaccgga ccggaggcct gagcacgatg
      901 agcgagaaca ctgtggaaga gaagtcgagc gtggagcgca actaccgcaa gacccgccag
      961 ggcatggtgg tcagcgacaa gatggacaag accgttgtcg tcgaggtcga ggaccgcgtc
     1021 aagcacgccc tctacggcaa ggtcatgcgc aagagccaca aggtcaaggc gcacgacgag
     1081 cagaacgctg ccggcgtggg tgaccgcgtc ctgatcatgg aaacccgtcc gctgagcgcc
     1141 tcgaagcgct ggcgcgtcgt ggagattttg gagaaggcca agtgatccag caggaatccc
     1201 gcctcaaggt tgccgacaac accggcgcca aggagatctt gtgcatccgc gtgctcggtg
     1261 gctccggccg ccggtatgcc ggtatcggcg acacgatcgt cgcgaccgtc aaggacgcca
     1321 tccccggcgg caacgtcaag aagggtgacg tcgtcaaggc cgtcgtcgtg cgcaccacca
     1381 aggagcgccg ccggccggac ggcagctaca tccgcttcga cgagaacgcc gccgtcatcc
     1441 tcaagaacga tggtgatcct cgtggcaccc gcatcttcgg cccggtgggc cgcgagctgc
     1501 gtgacaagcg cttcatgaag atcatctcgc tggcaccgga ggtgctctga ccgccatggg
     1561 cacaatgaag atcaagaagg gcgacaccgt ccaggtgctc accggcaagg acaaggccaa
     1621 gcagggcaag gtcatcgccg tcgacaccga gaaccagcgc gtgaccgtcg agggcgtcaa
     1681 tcgcgtgacc cggcacgtca agccgggcac ccgcggcacc ggcggcctcg tcgtgcagga
     1741 ggcgccgatc cacgtcagca atgtcgcggt catcgacccc accgacaaga agccgacccg
     1801 cgtcaagacc cgcgaagagc aggtcgagcg cgacggccgc accaagacca cccgcgtgcg
     1861 ggtctccgcg cgctccggca aggacatctg atgactgaga ccgcagcccc cgccgccaag
     1921 ccgcggctga agacgcgcta ccaggacgag atcaagtcgg ccctgcagga ccagttcaac
     1981 ttcggcaacc cgatgcaggt ccccggcgtc gtcaaggtcg tcgtcaacat gggtgtcggc
     2041 gacgccgcca aggactccaa gctgatcgag ggcgcgatca aggacctgac cgcgatcacc
     2101 ggccagaagc cgcagatcac caaggcccgc aagtcgatcg cccagttcaa gctgcgtgag
     2161 ggtatgccga tcggcgccca cacgacgctg cgcggcgatc gcatgtggga gttcctcgac
     2221 cgcctggtca cggtggcgct gccgcgtatc cgtgacttcc gcggcctgtc gccgaagcag
     2281 ttcgacggcc ggggcaacta caccttcggc ctgaccgagc agtcgatgtt ccacgagatc
     2341 gaccaggaca agatcgaccg cgtgcgcggc atggacatca cggtcgtgac gaccgcgacg
     2401 aacgacgacg agggacgcgc gctgctcaag gcgctcggct tccccttcaa ggagaactga
     2461 catggccaag accgccctga agaacaaggc tgccaaggcc cccaagttca aggttcgcgg
     2521 ctacacccgc tgccagcgtt gcgggcgtgc ccactccgtc taccgcaagt tcggcctgtg
     2581 ccggatctgc ctgcgcgaga tggcgcaccg cggcgaactg cccggcgtga ccaagtccag
     2641 ttggtagaca ccgaaaggcg aggagcttcc ttcggtcgca cggcactcga tgttccgggc
     2701 aaccaagctc gcttcgctcg acggttgccc ggtccgctca cgcggatcga gctggtaacc
     2761 cagccaccca cccagaccac cactacatcg caggtccgca cccggcatac cgggagcgga
     2821 aaccgtggtg agagagggcg aatcagccca tgaccatgac tgacccgatt gcggacatgt
     2881 tgacccgcgt ccggaacgcc aactcggcgc accacgacga ggtctccatg cccttttcga
     2941 agctgaagtc gcacattgcc gagatcctcc aggccgaggg ctatatcgcc tcctgggcgg
     3001 tcgaggacgc cgaggtcggc aagaccttga cgatcaacct caagtacggc cccaaccggg
     3061 agcgctccat cgccggcatc cgccgcgtga gcaagcccgg cctgcgggtc tacgcgaagt
     3121 ccaccaacct gcccagggtg ctcggtggcc tcggcatcgc gatcatttcc acgtcgtccg
     3181 gcctgctcac cgacaagcag gcggctagca agggtgtggg tggggaagtc ctcgcctaca
     3241 tctggtaagg gagcctcgac atgtctcgaa tcggacgtat gccggtgacc gttcccgcga
     3301 acgtcacggt cacgatcgac ggccagcagg tcaccgtcaa gggccccaag ggcgagttga
     3361 cgcacaccgt cgccgagccg atcacggtcg cgcagggcga gggcaacgtc gaggtcactc
     3421 ggcccaacga cgagcgcacc gcgcgctcgc tgcacggcct gacccgcacc ctgatcaaca
     3481 acatggtcct cggggtcacc gacggctatg agaaaaagat ggagatctac ggcaccggtt
     3541 atcgcgtcat ggcgcgcggt tccaacctgg agttcgctct cggctacagc cacccgatca
     3601 ccgtcgagcc gccggccggc atcaccttcg cggtcgagaa ccagacccgg ttctcggtcc
     3661 agggcatcga caagcagctc gtcggtgagg tcgccgccaa cattcgcaag ctgcgcaagc
     3721 ccgaccccta caagggcaag ggtgtccgct acgcgggcga gcagatccgc cgcaaggtcg
     3781 gaaaggctgg aaagaagtag tcatggcaat gatcgtcaag cgcggcaagt ccaaggccgc
     3841 cgcccgtggc cgtcgccacg accgcatccg caagcacgtc gccggcacca ccgagcggcc
     3901 ccgcctcgtc gtctcgcgca gcagccggca cctgttcgtg caggtcgtcg acgacaccgt
     3961 cggcaagacg ctggccagcg cctccaccat ggaggccgac ctgcgcgcct tcgacggtga
     4021 caagaccgcc aaggccaaga aggtcggcga actgctcgcc gagcgcgcca aggccgccgg
     4081 cgtcgagtcg gtcgtcttcg accggggcgg caacaagtac cacggtcgcg tcgccgccat
     4141 cgccgatggc gcccgccagg gaggcctgag tctctgatgg ccaacaacac tgagatgagg
     4201 aacatctgat gcccggaccc cagcgtcgag gcaccggcgc cgccgaccgc ggcggcgacc
     4261 ggcccgagcg cggcgagcgt cgcggccgcg acggtgaccg tcgcgacaac cgtcgcggcg
     4321 atgccgcaga gaagtcccag tacctcgagc gcgtggtgac catcaaccgc gtggccaagg
     4381 tcgtcaaggg tggtcgtcgc ttcagcttca ccgctctcgt cgtcgtcggc gacggtgacg
     4441 gcaccgtcgg cgtcggctac ggcaaggccc gtgaggtccc cgccgccatc gccaagggtg
     4501 tcgaggaagc caagaaggcc ttcttcaagg tgccgcgcat cgccggcacc atcccgcacc
     4561 ccgtccaggg tgaggccgcc gcgggcgtgg tcctgctccg cccggccgcc cccggtaccg
     4621 gtgtcatcgc cggtggcccg gtgcgcgccg tcctggagtg cgcgggcgtc cacgatgtgc
     4681 tgtccaagag cctcggctcg gacaactcca tcaacatcgt ccacgcgacc gtggccgccc
     4741 tgaaggacct cgagcgtccc gaggccgtcg ccgctcgtcg tggcttgccg ctggacgaag
     4801 tagccccggc cgccatgctg cgcgcccagg ccgccgccac cgccggcgca aaggccggtg
     4861 catgatggca cgtctgaagg tcacccaact caagggcatc gtcggcgaga agcacagcgc
     4921 gcgggagacc ctgcgcacgc tggggctgaa gcgcatcggc gatgtcgtcg tcaaggagga
     4981 ccgccccgag atccggggca tggtccgcac cgtccgtcac ctggtgacgg tcgaggaggt
     5041 cgagtgatgg ctgacaaggc caaggacacc gccgccacgg cgggcaccca cgctttgaag
     5101 gtgcaccacc tgcgtcccgc cccgggagcc aagaccgcca agacccgcgt gggtcgcggt
     5161 gagggctcca agggcaagac cgcgggtcgt ggcaccaagg gcaccaaggc ccgctaccag
     5221 gtgccggcgc acttcgaggg tggctccacc gccatccaca tgcgcctgcc caagctgcgc
     5281 ggcttcaaga accccttccg caccacctat caggtcgtca acctggacaa gatctcggcg
     5341 ctctaccccg acggtggcgc tgtgacggtc gaggacctgg tggccaaggg cgccgtccgc
     5401 gccggcgagc cggtcaaggt gctcggtacg ggcgagatca cggtcgcggt gcaggtcacc
     5461 gcggacgctt tctccgcctc cgccaaggag aagatcgagg ccgccggcgg ctccaccgcc
     5521 accgcctagg gctttccggc aatgagccaa cgggtatgcc gtgtggtgag gtctttcggg
     5581 aactcaccac acggcatacc ccgttaagta gccgatctgg ctcggttggg cgcggcacgc
     5641 gccgccgggg tagattgcac cgggagagaa cacctggctc tcccgcgcat gacgcatccc
     5701 gccgccgcac ctggcgaacg ggaggaggga cccgaccttg ctttccgcct tcggccgcgc
     5761 gttcaagacg ccggacctac gcaagaagct gctgttcacg ctcttcatca tcgccatcta
     5821 ccggctcggg gcgcacgtcc cgacgcccgg cgtgagctac ccgctggtgc agcagtgcat
     5881 ccagaacgcc ccgacgggcg gcggcttcct cgggctggcc aacctcttca gcggcggtgc
     5941 gctgctgcag ctgtcgatct tcgcgctggg catcatgccc tatatcaccg cgagcatcat
     6001 cgtccagttg ctcaccgtcg tcattccccg gttcgagacc ctcaaaaagg aaggtcacgc
     6061 ggggcagcag aagatgacgc agtacacgcg ctatctgacg atcgggctgg cgatcctgca
     6121 gagcgccacc ctggtcacct tcgcccagaa cccctcctct ttgatgcagg gctgcgagcg
     6181 ggtcctcgtc gacgacaaga tctccacgat cctcatcatg gtgctgacca tgacggccgg
     6241 caccggcatg atcatgtggc tcggtgagct gctcaccgac aagggtgtcg gcaacgggat
     6301 gtcgatcctc atcttcacct cgatcgtcgc cagcttcccc ggatcgctgt ggctgatccg
     6361 ccagcagaac ggctggggca tcttcctggc gatcatcgcc atgggcttcc tgatcatcgc
     6421 gctggtggtc ttcgtcgagc agagccagcg ccgcataccg gtgcagtacg cgaagcggat
     6481 ggtcggccgg cagatgtacg gcggcacctc gacctatatc ccgatcaagg tcaacatggc
     6541 caacgtcatc ccggtcatct tcgcggcctc gctgatggcg ctgccgatga tgatcgccca
     6601 gttcaacacc aacccgacgg gcgccagccc gggctgggtg acgtggatca acaacaccct
     6661 ggtcaacggg gatcacccga tctacatgat cctctacaca cttttgatcc tgttcttcac
     6721 cttcttctac gtctcgatta ccttcaatcc ggaggaagtc gcagacaaca tgaagcgcta
     6781 cggagggttc atccccggga ttcgagccgg caaaccgacg gccgagtacc tgcagtacgt
     6841 cctgacccgg atcactgtcg tcggcgcgat ctacctggcg ctcatctccc tcatcccgct
     6901 gatcgcgatc gccgtgatca acgccaacca gaacttcccg ttcggtggca cgtcgatcct
     6961 gatcatggtg ggtgtgggtc tggagaccgt caagcagatc gaggcgcagc tgcaccagcg
     7021 ccactacgaa ggcttcctgc gctaaccatc ggatccatcg acagctgaac aaaggactga
     7081 cacacgatga tgcgcctgat catcttcggg ccgcccggcg ccggcaaggg cacccaggct
     7141 tcccggatcg ccgcgcgcta cggcatcccg gcgatctcca ccggagacat cttccgggcc
     7201 aacatcaaga acgagacccc gttggggctg caggtcaagg agatcctcgc ctccggtggg
     7261 tatgtcagtg acgagatcac caacgcgatc gtcgcggacc gtttggcgca ggaggactgc
     7321 gcgccggggt tcctgctcga cggctacccc cgcacgtcgg atcaggtgta tgcgctcgac
     7381 gcgatgctgg ccgaacgtgg cggcgaactc gacgcggtcc tcgagctgac ggtcgacgag
     7441 gacgaggtcg tcgaccgact cctcaagcgg gccgagacgg aggggcgcgt ggatgacacc
     7501 gaggaggtca tccgcgaacg tctggccatc taccggcgcg agaccgcccc gctggcggcg
     7561 atctatctcg agcgcggtct gctcgtgcag gtcgacggtc tcggcgagat cgacgaggtc
     7621 agcgagcgca tcggcgtggc gttggacgag cgtcagcact aggccgcggc ggcacggcac
     7681 gatgacggca tgatgttcgg ccgcgaacga ctcgagacca agactcgaga ccaggtgcgt
     7741 gcgatgcgcg cacctggtct cgtcgtgggc caatgcctgg cgatgctgcg cgacgccgcg
     7801 cagccggggg tgaccaccct cgaactggac accctcgcgg agcgcttcat ccgcgaccac
     7861 ggcgggcggc cgagcttcgc cgaggtgccg ggctaccggc ataccctgtg cacctccgtc
     7921 aacgaggaga tcgtccacgg tatcccgcgg gagacacgcg tgctcgccgc gggcgacctc
     7981 ctctccatcg actgcggcgc catcgtcgac gactggcacg gcgacgccgc gatctcgctg
     8041 atcgtcggcg ggcgggaggc ggcgcggccc gaggacctgc agctgctgga cgccaccgag
     8101 gagtccctct gggcgggcat cgccgcgatg cgggtcgggc gcgacctctt cgaggtcggc
     8161 gccgcgatcg agaagtcgat cgtcgcctcc ggcaaggagc acgaccgcgt cttcgggatc
     8221 gtcgaggatt atgtcgggca cgggatcggc cgcgagatgc acatgtcgcc gaatgtgccc
     8281 aactacgccg tgcgcggcaa gggcccggtc gtcaagaacg ccgccactgt cgccatcgag
     8341 ccgatgatca ccctcggtga ggaggcgaac gagactctgg ccgaccagtg gacagtcgtc
     8401 acccgcgacc ggatgcgcgc ctgccactgg gagcacaccg tcgcctgcca cgacggcggc
     8461 atctgggtgc tcaccgccct cgacggcggc gccgcccgct tgggcaccgc atacgccccg
     8521 ctcgactgac tcggtccttc atatgcagga cgccgccgcg gtaaaacgcc cggcgcggct
     8581 tgtagaggtc ccgtgccccg gactacacga agtcccgagc tctgagccgg gcaagcgggg
     8641 ggcgggggcg gggaagcgac ttcatgtcgt cgcaggcacg ctcggcaacc tctgattgcc
     8701 cggccctcga ccttctcgct gcggccacgg ctgcatggtt tgacgatcgg cggcgtggcg
     8761 gctcccgagc gctacattgt cggggtgcga ttcgacacca cctatgccgg actccctgcc
     8821 gaggtcgtcg gagttgtcga ggccgccgag gccggtgggt atgacgggct cacgatcgcc
     8881 gagttgcagc acgatccgtt cgtgacggcg acgctcgcgt gcgcgcagga gagtcagctg
     8941 cggatcggca cctcagtggc catcgcgttc gcgcgctcgc cgatgaccgt ggcgcagacg
     9001 gcgtgggatc tgcagcggct ctccggtggc cggttcgcgc tcgggctggg cagccagatc
     9061 gagccgcaca tcacccggcg gttctcgatg ccgtggagca agccggcggc gcggatgcgc
     9121 gaatacatcg cggcggtcaa cgcgatctgg gacgcctggc aggacgggac gcggctgaac
     9181 ttccgcggcg acttctatac gcacacgctg atgacaccga tgttcaaccc ggggccgttg
     9241 cgcgtctccc ggccacgaat cctgctcgcc gccgtcggac cgctgatgat cacggtcgcg
     9301 gccgaggcgg cggacgggtt gatcgtgcat cccttcacga ccctgggcta cctgaccgac
     9361 gtcctcgagc cgctcgtggt ggacaagctc ggggagcgcg accgatccga cttcgaactg
     9421 atcgggcagg tgatggtggc cagcggacgg gaccagtcgc agatcgctgc ctcggtggcg
     9481 gcggtgaagg cgcagctggc gttctacggg tccacaccgg cataccgccc cgtccttgaa
     9541 caccacgggc tcggcgagct cggcgacgaa ctcaaccggc tctccaaacg gggggagtgg
     9601 accgcgatgg gggagcggat cgacgacgcg acgttcgcgt tgttcgcggt gcgtgggacg
     9661 ccgcaggagt gcggcgcgga tctggtgcgg cgcttcggtg cgacctacga ccggctctcc
     9721 ctgactctga tgggggcgag cgacgccgag ttggcggccg gggtggtcgg cgcggctcgg
     9781 gcgtcgggct gagggcgccg ctgcgggtct ggcgtagcgt ctgcatgaag gagatgccct
     9841 ccaggacgga atgcccctcg cgatgtgcgc gttgcacccc acaggcccgc tgacgtccac
     9901 gaatccagga gtactcccat ggccacgcgc aacctgaccg ccaccgacat ccagtccact
     9961 gtcaccgaca acgacatcgt tctcgtcgac ttctgggcct cgtggtgcgg cccgtgccgc
    10021 cagttcgccc cggttttcga gaaggcgtcc gaggagcacg cggacatcat cttcggcaag
    10081 gtcgacaccg aggccgagca ggcgctcgcc gggcaactca acatcacctc catccccacg
    10141 ctgatggcct tccgcgaggg catcctcgtc ttcaaccagg ccggagcgct gcccgccgcc
    10201 gggctgaacc aggtcatcga ggccgtcaag ggcctggaca tggagcaggt ccgcagccag
    10261 ctggtcccct ccccggagag ctgagcaccc aaccccgata cctcctgcgg cgtcatcgag
    10321 tcatccagga cgatcgcagg aggtatcgtc atgtccggca agagggatgt cttcctgggc
    10381 gatgtcgcga gccgaggtct accggtggaa ttggcgcacc gggctggtga ctctcgtcag
    10441 tacgcgggtg accggcgcgg aaggcgtcta cctgcacagc accgggcaga tcgagcggac
    10501 gcccgcggct gcctgatcca cgtctcccgg cgtcgccccg cggcggccgg ccgcgatttc
    10561 gtggccggcc gcgtcctcgc gtatcatgga gtgtcggtca cgtgtgcgca tgcgctctcg
    10621 cgtctgctca cccagggccg atttcgtatg gccggtccca gcacgtcagg ggggtcggtc
    10681 gccaaccagg attgacggag gatatggcca agaaggacgg tgtcatcgag gtcgagggca
    10741 cgatcgtcga ggcgctcccc aatgcgatgt ttcgcgtgga gctcagcaac ggtcacaagg
    10801 ttctcgccca catctcgggc aagatgcgtc agcactacat ccggatcctc cccgaggacc
    10861 gggtcgtggt ggagctcagc ccgtacgacc tgacccgcgg gcgcatcgtt ttccgttacc
    10921 ggtagcgcac gaacccggtg cccatcaggc catcgggaca acagccgatc gagaaaagac
    10981 gaaggcaatg aaggttcagc cgagcgtcaa gaagatctgc gacaagtgca aggtcatccg
    11041 ccgccacggg cgcgtcatgg tgatctgcga caaccagcgc cacaagcagc gccagggctg
    11101 atcgcaccgc acgagccgca gggcttctcc cgacgacaac tgaagaaaat cgcagcaccc
    11161 gacccccgca ttcgggcggg acgtcacccc tggcccggag gccggggacg ggctggcacg
    11221 gcatacgaaa cgccgtgagc gaccacccga ccggcgctgc ttccatacct ccggttaaac
    11281 agaaaaggaa tgccatccca tggcacgtct cgttggagtc gacctgccgc gcgacaagcg
    11341 cgtcgaggtc gccttgacct acatcttcgg tgtgggtcgc acgacgtccc aaaaggcgct
    11401 ggaggccacg ggtgtcgacc cgtccatccg cgtcaaggac ctcgtcgatg accagctggt
    11461 cgcgttgcgt gactacctcg aagcgaacgt gaagatcgaa ggtgacctcc gccgcgaggt
    11521 tgcctccgac atccgccgca aggtcgagat cgggtcctac gagggcctgc gtcaccgccg
    11581 tggtctgccc gttcgcggtc agcggaccaa gaccaacgcc cgcacccgca agggccccaa
    11641 gcgcaccgtc gctggcaaga agaaggctgg taagtaatgc cccccaaggc acgcgcgacc
    11701 aaggttcgtc gcaaggtcaa gaagaacgtc gccgtgggcc aagctcacat caagtcgacc
    11761 ttcaacaaca ccatcatctc catcaccgac ccgaccggtg cggtgatctc gtgggcctcc
    11821 gccggtcagg tcggcttcaa gggctcgcgc aagtccaccc cgtatgccgc gcagatggcc
    11881 gccgaggcct gcgcccgccg cgccatggag cacggcatgc gcaaggtcga cgtcttcgtc
    11941 aaaggccccg gctcgggtcg tgagaccgcc attcgttccc tcaccgccgc tggcctcgag
    12001 gtcggtgcca tctccgatgt cacccccagc ccgcacaacg gtgtccgccc gcccaagcgc
    12061 cgccgcgtct gagcctttcg aaagcgagaa atagctaagt catggctcgt tacaccggcc
    12121 ccaccaccaa gaagtcccgc cgctacaagc tcgacctggt cggcggcgac aagaacttcg
    12181 acctgcgtcc cttcccgccc ggccagcacg gc
//
LOCUS       DAOPJG010000097        12127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5518_length_12127_cov_8.263088, whole genome shotgun sequence.
ACCESSION   DAOPJG010000097 DAOPJG010000000
VERSION     DAOPJG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12127
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5518_length_12127_cov_8.263088"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKU75_09900"
     CDS             complement(<1..1108)
                     /locus_tag="PKU75_09900"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPF81233.1"
                     /translation="MSERLSRLRTMREQALAGGGQARIDQQHAKGKLTARERLAILLD
                     EASFQELGALATHQLSDFGMDKHKVPGDGIITGFGKINGRRVAVFAQDFTVLGGSFSE
                     VQSHKISRIQDLAMESGIPLIGLNDSGGARVQEGVRSLAAYGEVFLRNVMASGVIPQI
                     SLIMGPCAGGAVYSPALTDFTIMVEGTSNMFLTGPDIVRTVTGEDVDAETLGGAAVHN
                     TRSGVAQFSAPTEQAALALAKTLLSYLPQNNNDDPPQITPYDSPDRMDEELNSIVPED
                     DTEAYDMREVLDRVFDADSVLEVHTDYAPNAIVAFARLDGKPVGIIANQPMVMAGALD
                     LNGSDKIARHISLCDMYNLPLVTFVDCPGYLPGVD"
     gene            1232..2809
                     /locus_tag="PKU75_09905"
     CDS             1232..2809
                     /locus_tag="PKU75_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF81234.1"
                     /translation="MGRRMQGIGFGMAAALAATLVPGVSRAAEDQVRAPVPVIDWQAC
                     TGAKQFRCATVAVPLDYDNPGGAKILLDLLKVPAARPSTKIGTLFVNPGGPGGSSTGF
                     APMASILLGSTVRNRYDVIGIDPRGVGASTPMKCVSDVPRPPGIAYLFPMTWAQAKPA
                     FIQAQWTRWACADQAAPIVRHMSTADTARDMDLMRQAVGEKKLNYYGISYGTQLGSTY
                     ASMFPSRVGRFVVDGVLDPIAWSTGDVVPAVQPFSTRLRSAKGAHEALMSALEDCDRV
                     GRAKCDFAGNASGKWQRLVSLAKAGKLTAYGSPLSYADLIGGVLGYLYATRYVGLDSA
                     LQEIWEENAGSGARASASAVADLRAAGERVRKAPYATPLLRGEVVSDAFSGVACSDTR
                     NPDSVTDWWRAGVEQDTKYPGFGSLWTWASASCSKWPIVTKSDSYFRPYGGATATPIL
                     VVGNTYDPATPIHGARKVASLFTGARLLTYDGWGHGAIGTGCVTRAFDRYYATGALPP
                     VGAVCPMDSGLWDGDAG"
     gene            2888..4444
                     /locus_tag="PKU75_09910"
     CDS             2888..4444
                     /locus_tag="PKU75_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF81235.1"
                     /translation="MGWIAAASTALAAGVAAPAAGTADAVAPAIAWQDCWAGWGTKCA
                     TVRVPLDYDNPRGATILLDLVKMPATGPQPRIGTVFVNPGGPGVRATDFVDYASRHLG
                     PTVTARYDVIGIDPRGVGQDSMMLCHNDNPEPTPAPPPFPVTWTQSAAVWRSAQWVRT
                     GCKENPNPIVAHMSTADTARDMDLIRRALGEPRLNYYGASYGTYLGATYASMFPDRVR
                     RMVLDGVVDPVAWATGATGEAATLPVTTRVRGAEGAYTGLVAALRECDRLGRRQCAFA
                     GNAPGKWRQLLARAKAGGLQVNERRMTYPALVQSTLGMMYEDDYSLLAQSLQAIWVEN
                     FRRVDRVDPRLVQALDRASRRVDEQPYAAPSATRGPVTETVDAFHGVACADSRNPRER
                     IDWWRAGQAQDKQYPWFGSLWTWRSATCGNWPIVTKEDTWFRPFGGRTSGPILVVGNT
                     YDPATPIHGARAVVRRFDRARLLTYAGWGHTAIGNSCVTAAFDTFFASGVMPAPGAVC
                     RMDGPLFSGG"
     gene            complement(4509..7277)
                     /gene="ppc"
                     /locus_tag="PKU75_09915"
     CDS             complement(4509..7277)
                     /gene="ppc"
                     /locus_tag="PKU75_09915"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HPF81236.1"
                     /translation="MTIPAESSEAAEPADALLRRDIRRLGELLGDTLVRQESPAVLDA
                     VEEVRRLTKRAQGGDEDARRDLTKRLAAVDLPTATLMVRAFATYFHLANIAEQVDRAA
                     HLATRPEDADWLVAAMDKIVEARGPAALTTALSKLSVRPVYTAHPTEASRRTALLKLR
                     EIADALIAHRTNPTPRSERRRDQRLAEIIESMWQSDDLRVDQPQVTDEARNMLFFVGG
                     LLEETVPNLLADLADEAARRGAFLPPTARPLIFGNWIGGDRDGNPNVSPATTLDVLVM
                     QHAVGIRGLIDMVQQCVDEVSMSTKLVGVSDELAASLERDLAILQIEDRIRRLNVEEP
                     YRLKLSCIRQKLINTRDRIAARVDSRPGFDYVGRDELVADLMLIRDSLLAHRGELVAG
                     GALATLIRTASAFGLQVATMDVREHSGAHHHVIEQLADRVDDEPGSYAALAREDRMAW
                     LAEELTSRRPLAPFPPPLDADGSRTFETFRAIRAAQKWFGPEIIESYIVSMTQGADDI
                     LAPVVIAREAGLVDLPTDQGRPVRADIGFVPLLETVEELRSAGEILDALLSVEAYREI
                     VRARGDVQEVMLGYSDSNKDAGITTSQWEIHRAQRVLRDTCTRHGVQLRLFHGRGGSV
                     GRGGGPTHEAILAQPFGTLSGEIKVTEQGEVISDKYLLPALARENLELTLAAVMEASA
                     LHTTAWVDPAQLTQWDETMSSVSAAAFTAYRALVDNPQLPTYFAQSTPFEQLGALKIG
                     SRPSKRPDSGQGLSGLRAIPWVFGWTQSRQIVPGWFGVGSGLRSAIDAGRLEELRAMY
                     AGWHFFKTFISNVEMMCKKTRLDIAAHYVGSLVDPELHPIFEQIQQEYARTVDAVLQI
                     TGEGELLEAQPVLKRTLEVRDRYLDPITYLQVSMLARMRAGDTDPQLERALLLAVNGV
                     AAGLRNTG"
     gene            7368..8972
                     /locus_tag="PKU75_09920"
     CDS             7368..8972
                     /locus_tag="PKU75_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF81237.1"
                     /translation="MTAAWFRDAVIYEIYPQSFHDTNADGIGDLRGVIERLDYIAALG
                     VDAIWFNPCFESPFVDAGYDVADYLRVAPRYGTNDDLVELVDKARERGIRILLDLVVG
                     HTSTAHAWFQEELAASGPNPDGDRYIWTTEDPGDGWGIDLPGIPAWVASPGPRPGWYL
                     KNFYDEQPALNFGWTRTDPAQPWRDAIDAPGPTRNRQALKDILDFWLSRGIAGFRVDM
                     AFSLVKDEYLTEGRLASARIWQEIREWLDTVYPDAVLIPEGDEPRVGGRRAFDADFAL
                     VIFEAHASLFDDHSAGTLPWHTPHEPFFAAEGAGSTETFLRLWEGWRAEDPERSILLA
                     TADHDFNRLRCGGRTPEQLGAAFTFLFTWGSVPSLYYGDEIGMRYLPGMPNVEGAVCN
                     PLYNRAGARTPMQWDDGPNAGFSTAAPKALYLPIDPEPDRPTVAAQDGVPGSTLTLVR
                     DLLALRRATPALQGHEPTRVVHEGYPFAWMRGHSHLVVVNPRRLAAVLESPEAVGAQL
                     LWGSGVTVTESGITLEGFGYGVFVVA"
     gene            8969..9919
                     /locus_tag="PKU75_09925"
     CDS             8969..9919
                     /locus_tag="PKU75_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPF81238.1"
                     /translation="MTVSHPLIPESLRDLLADREPEPDVSGAEWLRRLPSLIDASLAR
                     WDLVPDGEARHGECALVLPVRLQGNDFADSAPTREAILKLTWPHDEARLEHLALRHWN
                     GRGAVRLLAADPASYGLLLERLDADRELTSVSILEACDVIGGLMRVLDQPAGEQYVLV
                     SDKAQRWDAQLRHGTPLVPRRLTDQAAATLAHLVTGEVDGRLVHEDLHDMNVLAPLVL
                     SRGDWLAIDPKPVSGEWAYAVAPIVWNRTDATAAAYNQRTHVRLRADIVADAAGLDPD
                     RVTHWTFVRLVLNAVWAAAYAPASDDFRSRMIALSKAFSD"
     gene            complement(9929..10696)
                     /locus_tag="PKU75_09930"
     CDS             complement(9929..10696)
                     /locus_tag="PKU75_09930"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPF81239.1"
                     /translation="MNVLDRFSLQGRRALVTGATRGLGRAFAQALAEAGADIVVHGRD
                     EAAAEQAKTELTATGRRVEVVLGELTDRPTVDAVTAQAVELLGGIDILVNNAGTCIHA
                     PALEVTDEQWASVLDTNVTALWYMSQAVGAHMVERGSGSIVNIGSISGMIVNRPQMQP
                     AYNASKAAVHQLTKSLAAEWAPHGVRVNAVAPGYVKTDMAPVDEPQFQRYWIEDAPQQ
                     RYASPDEIAPAVLFLASDAAAFVTGSVLVIDGGYTVY"
     gene            complement(10752..11138)
                     /locus_tag="PKU75_09935"
     CDS             complement(10752..11138)
                     /locus_tag="PKU75_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF81240.1"
                     /translation="MASRASNFCIDAHDPYAQTQWWAQVLEDFEPEADWDMKPGDDEC
                     GLAGPDDRFLLFLKVPEPKTVKNRMHICLRPTDRHRDEEVDRILGLGATLVNDLRDGP
                     DEGWAVLADPEGNEFCVLSRYKGKPA"
     gene            11305..>12127
                     /gene="fbaA"
                     /locus_tag="PKU75_09940"
     CDS             11305..>12127
                     /gene="fbaA"
                     /locus_tag="PKU75_09940"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPF81241.1"
                     /translation="MPIATPEVYRDMLDRAKDGSFAYPAINITSSSTINAAIRGFAEA
                     GSDGIIQVSTGGAEFASGSNIKNMVTGSLALAAFAEEVAKNYPVNIALHTDHCPEPKL
                     DGFVRPLIEASAARVKAGGLPIFQSHMWDGSAVPLDQNLQIAQELLEKCQAANIILEI
                     EVGVVGGEEDGVANEINDKLYSTPEDAVATVKALGAGEKGYYMTALTFGNVHGVYKPG
                     NVKLRPDVLKAAQEAAAGELGLAADAKPFHLVFHGGSGSLPEEISAAVDYGVVKMN"
BASE COUNT         1854 a         4032 c         4275 g         1966 t
ORIGIN      
        1 ggtcgacccc gggcaggtag ccgggacagt cgacgaaggt gaccagcggc aagttgtaca
       61 tgtcacacaa cgagatgtgc cgggcgatct tgtccgagcc gttgagatcg agcgcgccgg
      121 ccatcaccat cggctggttg gcgatgatcc cgaccggctt gccgtccagc cgcgcgaagg
      181 cgacgatcgc gttgggggcg tagtcggtgt gcacctccag gacactgtcg gcgtcgaaga
      241 cccggtcgag cacctcgcgc atgtcgtagg cctcggtatc gtcttccggc acaatcgaat
      301 tgagctcctc gtccattctg tccggggagt cgtacggcgt gatctgcggc gggtcgtcgt
      361 tgttgttctg cggcagatag ctgagcaagg tcttggccag ggcgagcgcg gcctgctcgg
      421 tgggtgccga gaactgggcg acaccactgc gggtgttgtg caccgccgca ccgccgaggg
      481 tctcggcgtc gacgtcctcg ccggtgacgg tgcgcacgat gtccggcccc gtgagaaaca
      541 tattgctcgt gccctcgacc atgatcgtga agtcggtcag ggcgggcgaa tagacggcgc
      601 cgccggcgca ggggcccatg atcaacgaga tctgcgggat gactcccgac gccatcacgt
      661 tgcgcaagaa cacttctccg tatgccgcga gcgagcgcac cccctcctgc acccgtgccc
      721 cgccggagtc gttcagcccg atcagcggta tgccgctctc catcgccagg tcctggatgc
      781 ggctgatctt gtgggactgc acttcggaga aggacccgcc gaggacggtg aagtcctggg
      841 cgaagacggc cacgcggcgg ccgttgatct tgccgaagcc ggtgatgatc ccatcgccgg
      901 ggaccttgtg cttgtccatg ccgaaatcgc tgagctggtg ggtggccagc gcgccgagtt
      961 cttggaagga cgcctcgtcc agaagaatcg caagccgttc tcgcgcagtc agtttgccct
     1021 tggcgtgctg ctggtcgatg cgggcctgcc cgccaccggc gagcgcctgc tcgcgcatcg
     1081 tgcgcagtcg agacagcctc tcggacaccg caatctcctt cgtggggggc acttcagcct
     1141 attcccgggt attgcgcgaa accgcggaag gcacaagggt ggtggtgacg gccacaaccg
     1201 aggtggtcgc gtcagcgaat ggggattcgt cgtgggacgc aggatgcagg ggatcgggtt
     1261 cggtatggca gcggcgttgg ccgcgacctt ggtgccgggg gtgagccggg cggccgagga
     1321 tcaggtgcgg gcgccggtgc cggtgatcga ctggcaggcc tgcacgggcg cgaaacagtt
     1381 cagatgtgcg acggtcgccg tgcccctcga ctacgacaac ccgggcgggg cgaagattct
     1441 gctcgatctg ctcaaggttc cggcggctcg gccgagcacg aagatcggca cgctcttcgt
     1501 caatccaggg ggcccgggcg gcagttcgac cggctttgcc ccgatggcct cgatcctgct
     1561 cgggtcgacc gtgcgcaacc ggtatgacgt gatcggcatc gacccgcgag gggtgggggc
     1621 ctcgaccccg atgaagtgcg tgagcgacgt gccgcgtccg ccgggcatcg cctatctgtt
     1681 cccgatgacc tgggcacagg ccaagccggc cttcatccag gcccaatgga cgcggtgggc
     1741 ctgcgcggac caggctgcgc cgatcgtgcg gcatatgtcg acggccgaca ccgcccgcga
     1801 tatggacctg atgcggcagg cggtcggtga gaagaagctg aactactacg gcatctccta
     1861 cggcacgcag ctcggctcga cctatgcctc gatgttcccc tcgcgggtcg ggcggttcgt
     1921 cgtcgacggc gtcctcgacc cgatcgcctg gtcgaccggg gacgtggtgc ccgcggtgca
     1981 gccgttctcg acccggctgc gcagcgccaa gggcgcgcac gaggcgttga tgtcggcgct
     2041 ggaggactgc gatcgggtgg gccgggccaa atgcgacttc gccggcaacg ccagcggcaa
     2101 gtggcagcgc ttggtgtcct tggccaaggc cggcaagctc accgcatacg gcagtccgtt
     2161 gagctacgcg gacctgatcg gcggcgtgct gggctatctc tacgcgacca gatatgtcgg
     2221 cctcgacagc gccctgcaag agatctggga ggagaacgcc ggcagcgggg cgcgggccag
     2281 tgcctcggcc gtcgccgatc tgcgggcggc gggggagcgg gtgcgcaagg ccccgtatgc
     2341 gacgccgctg ctgcgcggcg aagtcgtctc cgacgccttc agcggcgtcg cgtgctccga
     2401 cacccgcaat cccgactcgg tcaccgattg gtggcgggcc ggtgtcgagc aggacacgaa
     2461 gtatcccggc ttcgggtcgc tgtggacgtg ggcgtcggcc agttgctcga agtggccgat
     2521 cgtgaccaag tcggacagct atttcaggcc gtatggcggt gccacggcga cgccgatcct
     2581 ggtggtgggc aacacctacg acccggccac gccgatccac ggcgcgcgca aggtggcgag
     2641 cctgttcacc ggcgcccgcc tgctgacgta tgacgggtgg gggcacggcg cgatcggcac
     2701 cggctgcgtg accagagcct tcgaccgcta ctacgccacc ggggcgttgc cgccggtcgg
     2761 cgcggtgtgc ccgatggact ccggcctgtg ggacggtgac gccgggtagg ccgggggacg
     2821 cggcatactc gtatccgata cggcgcgaag cgcctgacga gaaacgggga cgaagatgtc
     2881 gcgtcgtgtg gggtggattg ctgccgccag cacggcgctg gcggccggtg tcgcggcgcc
     2941 ggcggccggg acagctgacg cggtggcacc cgccatcgcg tggcaggact gctgggccgg
     3001 ctggggcacc aagtgcgcca ccgtgcgcgt gccgctggac tacgacaacc cgcgcggcgc
     3061 gacgatcctg ctcgacctgg tcaagatgcc cgcgaccggc ccgcagccgc gcatcgggac
     3121 ggtcttcgtc aaccctggtg gtccgggcgt gcgcgcgacg gacttcgtcg actatgcatc
     3181 ccggcatctc ggccccaccg tgacggcgcg gtatgacgtc atcgggatcg accctcgcgg
     3241 cgtgggccag gactcgatga tgttgtgcca caacgacaat ccggaaccaa cgccggcacc
     3301 gccaccgttc cccgtgacct ggacgcaatc ggcggcggtc tggcgcagcg cgcaatgggt
     3361 gcgcaccgga tgcaaggaga atcccaaccc gatcgtcgcg cacatgtcga ccgccgacac
     3421 cgcccgcgat atggacctga tccgccgggc gctcggcgaa ccgcggctga actactacgg
     3481 agcgtcctat ggcacctatc tcggggcgac ctacgcctcg atgttccccg accgggtgcg
     3541 acggatggtc ctcgacggcg tggtggaccc ggtggcgtgg gccaccggag cgaccggaga
     3601 agccgcgacc ctgccggtga ccacgcgggt gcgtggcgcc gaaggtgcct acaccgggct
     3661 cgtcgcggcg ctgcgcgagt gtgaccggct gggtcgccgg cagtgcgcct tcgccggtaa
     3721 tgcgccgggc aagtggcgcc agctccttgc acgggcaaag gccggcggct tgcaggtcaa
     3781 cgagcgccgc atgacctatc cagcccttgt gcagtccacc ctcgggatga tgtacgagga
     3841 cgactattcc cttctcgcgc agtcacttca agcgatctgg gtcgagaact tccgccgcgt
     3901 cgacagggtc gacccgcggc tcgtccaggc gctggaccgc gccagcaggc gcgtcgacga
     3961 gcagccgtat gccgccccgt cggcaacgcg ggggccggtg accgagacgg tggatgcctt
     4021 ccacggggtc gcgtgcgcgg acagccgcaa cccgcgcgag cggatcgact ggtggcgtgc
     4081 cgggcaagcc caggacaagc aatacccgtg gttcgggtcg ctgtggacat ggcgcagcgc
     4141 gacctgcggc aactggccga tcgtgaccaa ggaagacacc tggttccgtc cgttcggcgg
     4201 gcggacgtcg gggccgatcc tcgtcgtcgg caacacctac gaccccgcga ccccgatcca
     4261 tggcgcccgg gcggtcgttc gccgcttcga ccgggcgcgg ctgctgacct atgccgggtg
     4321 gggacacacc gccatcggga actcttgcgt gacagcggca ttcgacacct tcttcgcatc
     4381 cggcgtcatg ccggcgccgg gggccgtgtg tcggatggac ggcccgctct tcagcggggg
     4441 ctgaaccaac ccgggacggc gtcgggctgc acggcctggc acgaacgggt gctgccccgg
     4501 tgtgtggcct acccggtgtt gcgcagtccg gcggcgacac cgttgacggc gagcaagagc
     4561 gcccgctcca gctgcgggtc ggtgtcgccg gcccgcatcc gggcgagcat ggagacctgc
     4621 agataggtga tggggtccag atagcggtcg cggacctcga gggtgcgctt gagaaccggt
     4681 tgggcctcca acagctctcc ctcgccggtg atctgcagga cagcatcgac cgtgcgggca
     4741 tactcctgct ggatctgctc gaagatcggg tgcagttcgg ggtcgacgag tgagccgaca
     4801 tagtgtgccg cgatgtccag ccgcgtcttc ttgcacatca tctcgacgtt ggagatgaac
     4861 gtcttgaaga agtgccaccc ggcatacatc gcccggagtt cctcgagacg cccggcatcg
     4921 atcgccgacc gcagccccga cccgacgccg aaccatccgg ggacgatctg tcgggactgg
     4981 gtccacccga aaacccatgg gatggcgcgc aatccggaca ggccctgtcc gctgtcgggt
     5041 cgcttcgacg gtcgcgaccc gatcttgagg gcgccgagct gttcgaatgg cgtcgactgg
     5101 gcgaagtagg tgggcagctg cgggttgtcg acgagggcgc ggtatgcggt gaaggctgct
     5161 gcgctgacgc tcgacatggt ctcgtcccac tgggtcagct gagcgggatc gacccacgcc
     5221 gtcgtatgca gcgcgctcgc ctccatgacg gcggcgaggg tgagttcgag gttctcccgt
     5281 gccagagccg gcaggaggta cttgtcggag atgacctcac cctgctcggt gaccttgatc
     5341 tcgccggaga gcgtgccgaa gggctgggcg aggatcgcct cgtgagtcgg gccgccgccg
     5401 cggccgacgc tgccgccgcg cccgtggaag aggcggagct ggacgccatg tcgtgtgcag
     5461 gtatcgcgca gcacccgctg ggcccggtgg atctcccact ggctcgtcgt gatgccggcg
     5521 tccttgttgg agtcggagta gccgagcatg acctcttgga cgtcgccgcg ggcccgcacg
     5581 atctcccggt aggcctcgac cgagagcagg gcatcgagaa tctctccggc agaacgcaat
     5641 tcctccacgg tctccagcag gggcacgaac ccgatgtcgg cgcgcaccgg ccgtccctgg
     5701 tcggtgggca ggtcgaccag cccggcctcc cgggcgatga cgaccggggc caggatgtcg
     5761 tccgcgccct gcgtcatcga gacgatgtag ctctcgatga tctccgggcc gaaccacttc
     5821 tgcgcggcgc ggatggcccg gaaggtctcg aaggtgcgcg agccgtccgc gtccagcggc
     5881 ggcgggaagg gggccagcgg ccgccggctc gtcagctcct cggccagcca cgccatccgg
     5941 tcctcgcggg ccagggcggc atacgacccc ggctcgtcgt cgacgcggtc ggcgagttgc
     6001 tcgatgacgt gatggtgagc accgctgtgc tcgcgcacgt ccatcgtcgc cacctgcagg
     6061 ccgaaggcgc tcgcggtgcg gatcagggtg gcgagggcgc cacccgcgac cagctcaccc
     6121 cggtgcgcca acaaggagtc gcggatgagc atgagatcgg cgaccagttc gtcgcggccg
     6181 acatagtcga acccggggcg cgaatccacc cgtgccgcaa tgcgatcgcg tgtgttgatc
     6241 agcttctggc ggatgcagga gagcttgagc cgatagggct cctcgacgtt cagccggcgg
     6301 atgcggtcct cgatctgcag gatggcgagg tcgcgttcga ggctggcggc gagttcgtcg
     6361 ctcacgccga cgagcttggt ggacatcgag acctcgtcga cgcactgctg caccatgtcg
     6421 atcagcccgc ggatgccgac ggcgtgctgc atgaccagca catcgagcgt ggtggcgggc
     6481 gagacattgg ggttcccgtc gcggtcgccc ccgatccagt tgccgaagat gagcgggcgt
     6541 gcggtcgggg gaaggaaggc gccgcgccgc gccgcctcgt cggccaggtc ggcgagcagg
     6601 ttcgggacgg tctcctcgag gaggccgccg acgaagaaga gcatattgcg ggcttcgtcg
     6661 gtgacctgcg gctgatcgac gcgcagatcg tcgctctgcc acatcgattc gatgatctcc
     6721 gcgagccgtt ggtcacgacg gcgctcggaa cgcggtgtgg ggttggtgcg atgggcgatc
     6781 aaggcgtcgg cgatctcgcg cagcttcagc agtgcggtgc gccggctcgc ctccgtcggg
     6841 tgggcggtat agaccgggcg caccgacagc ttcgacagcg cggtggtcaa ggcggccggg
     6901 ccgcgggcct cgacgatctt gtccatcgcc gcgacgagcc agtcggcatc ctcgggtcgg
     6961 gtggcgaggt gggccgcccg gtccacctgt tcggcgatat tggcgaggtg gaagtaggtc
     7021 gcaaaggcgc gcaccatcaa cgtcgccgtg ggcagatcga cggccgcgag ccgcttggtc
     7081 agatcacgcc gggcgtcctc gtcgccgccc tgggcgcgct tggtgagccg gcggacctcc
     7141 tcgaccgcgt cgagcacggc gggggattcc tggcgcacga gggtatcgcc gagcagctcg
     7201 ccgagccggc ggatgtcgcg gcgcagcagg gcgtcggcgg gttcggccgc ttcgctcgac
     7261 tcggcaggga tggtcatgcg cagcagagta tgtaaccgct ttcatctgcg gcaaccgcgc
     7321 ctcgccaggt gggcgtccgc agcggcgaag tggggaagga tgtgggcatg accgccgcct
     7381 ggttccgcga tgccgtgatc tacgagatct atccgcagtc cttccacgac accaatgccg
     7441 acgggatcgg agacctgcgc ggggtcatcg agcggctcga ctacatcgcc gcactgggcg
     7501 tcgacgcgat ctggttcaac ccgtgcttcg agtcgccctt cgtcgacgcc ggctatgacg
     7561 tggccgacta cctgcgggtc gccccgcgct atggcacgaa cgacgacctc gtcgaactcg
     7621 tcgacaaggc ccgggagcgc ggcatccgga tcctgctcga cctggtcgtc ggacacacct
     7681 ccaccgcgca cgcgtggttc caggaggaac tcgcggcctc cgggccgaac ccggacggcg
     7741 accgctatat ctggaccacc gaggatccgg gcgacgggtg ggggatcgac ctacccggca
     7801 tacccgcgtg ggtggcctca cccggcccgc gacccggctg gtacctgaag aacttctacg
     7861 acgagcaacc cgccctgaac ttcggctgga cccgcaccga ccccgcgcag ccgtggcggg
     7921 acgcgatcga tgcccccggc ccgacacgaa atcgccaggc gctcaaggac attctcgact
     7981 tctggctgag ccggggcatc gccggcttcc gcgtcgacat ggccttctcg ctcgtcaagg
     8041 acgagtacct gaccgagggg cggctcgcct cggcgcggat ctggcaggag atccgggagt
     8101 ggctcgacac ggtatacccg gacgccgtgc tgatccccga gggcgacgag ccgcgggtcg
     8161 gcggccggcg cgctttcgac gccgatttcg ccctggtgat cttcgaggcg cacgcgagtc
     8221 tcttcgacga ccattcggcg gggaccttgc cctggcacac gccgcacgag ccctttttcg
     8281 cggcggaggg agcgggctcg accgagacct tcctgcggct gtgggagggc tggcgggccg
     8341 aggaccccga acggtcgatc ctgctcgcca cggccgacca cgacttcaac cggctccgct
     8401 gcggcggtcg gactccggag caactcggtg ccgccttcac ctttctcttc acgtggggga
     8461 gcgtgccgag tctctactac ggcgacgaga tcgggatgcg ctatctgccc ggtatgccga
     8521 atgtcgaagg ggccgtgtgc aatccgctct acaaccgggc cggcgcgcgc actccgatgc
     8581 agtgggacga cgggccgaat gccgggttct cgactgccgc ccccaaggcg ttgtatctgc
     8641 cgatcgatcc ggagcccgac cgccccacgg tcgcggcgca ggacggggtt cccggctcga
     8701 cgctgaccct cgtgcgcgac ctgctcgccc tccgccgcgc cacgcccgcc ctccaggggc
     8761 acgagccgac tcgcgttgtt cacgagggct atccgttcgc ttggatgcgc ggccactccc
     8821 acctcgtggt ggtcaatccg cgcaggctgg cggcggtgct ggagtcgccg gaagctgttg
     8881 gtgcgcaact cctttggggc tcgggtgtga ccgtcactga gtcgggcatc actctggaag
     8941 gcttcggtta cggggtcttc gtggtcgcgt gaccgtgtcg catccactga tccccgagtc
     9001 gctgcgggac ctgctcgccg accgagagcc cgagcctgac gtctccggcg ctgagtggtt
     9061 gcgccggctc ccgtcgctca tcgacgcatc cctggcgcga tgggacctcg tgcccgacgg
     9121 cgaggcgcgg cacggggaat gtgcgctcgt cctcccggtc cggttgcagg gcaacgactt
     9181 cgcggattcg gccccgaccc gcgaagcgat actcaagctc acatggcccc acgacgaggc
     9241 ccgcctcgag catctggcgc tgcgccactg gaacgggcgc ggggcggtgc gattgctcgc
     9301 ggccgacccg gcgtcatacg gtctgctcct cgaacggctg gacgcggacc gggaactgac
     9361 gagcgtgtcg atcctcgaag cctgcgacgt cataggcggt ctcatgcgcg tcctggacca
     9421 gccggcgggt gagcaatacg tcctggtgtc cgacaaggcc cagcgctggg acgcccaact
     9481 ccggcacggc accccgctcg tgccgcgccg gctgacggac caagcagcag cgacgctcgc
     9541 ccacctggtg accggtgagg tcgacgggcg gctggtccat gaggatctcc acgacatgaa
     9601 tgttcttgcg cccctcgtcc tttcgcgggg ggactggctg gccatcgacc ccaaaccggt
     9661 gtctggtgag tgggcgtatg cggtggcacc gatcgtgtgg aatcgcaccg acgcgaccgc
     9721 cgcggcatac aaccagcgca cccacgtgcg cctgcgtgcc gacatcgtcg cggacgccgc
     9781 gggcctcgac cccgaccggg tcacgcactg gaccttcgtc cggctggtgc tcaacgctgt
     9841 ctgggctgcg gcctacgcac cggcctccga cgatttccgg tcccggatga tcgccctgtc
     9901 aaaagcgttc tcggactgaa ggattcgatc agtagacggt gtaaccgccg tcgatgacca
     9961 acacgctgcc ggtgacgaag gctgcggcgt cggaggcgag gaagagcacc gccggagcga
    10021 tctcgtccgg gctggcgtaa cgctgctgcg gcgcgtcctc gatccaatag cgctggaact
    10081 gcggctcgtc gacgggcgcc atatcggtct tgacgtagcc gggagccacg gcattgacgc
    10141 ggacgccgtg gggcgcccac tccgccgcga gcgacttggt cagttggtgc acggccgcct
    10201 tggacgcgtt gtatgccggc tgcatctgcg ggcggttgac gatcatcccc gagatcgatc
    10261 cgatgttgac gatgctcccc gagccccgct cgaccatgtg tgcaccgacg gcctgggaca
    10321 tgtaccacag ggcggtcaca ttggtgtcga gcaccgatgc ccattgctcg tcggtcacct
    10381 ccagcgccgg tgcgtggatg cacgtcccgg cgttgttgac gaggatgtcg attcctccca
    10441 acaactcgac ggcctgggcc gtgacggcat ccacggtggg ccggtcggtc aactcaccga
    10501 gcacgacctc cacgcggcgc ccggttgccg tgagctctgt cttggcctgc tcggccgccg
    10561 cttcgtcgcg tccgtggacg acgatgtcgg ccccggcctc cgccagggct tgggcgaagg
    10621 cgcgaccgag ccctcgggtg gcaccggtga cgagggcgcg gcggccctgg agggagaagc
    10681 ggtcgagaac gttcatgtgc tgactctttc ggtctgttgt cgttgtcgtt gtctttgtcg
    10741 gtggccggtg gctatgcggg cttgcccttg tagcgggaga ggacgcagaa ctcgttgccc
    10801 tccggatcgg caaggacggc ccaaccctcg tcgggtccgt cgcgcaggtc gttgacgagg
    10861 gtggcgccga ggccgaggat ccggtcgacc tcctcgtcac ggtggcgatc ggtggggcgc
    10921 aggcagatgt gcatccggtt cttgacggtc ttgggctcgg gcactttgag gaagagcagg
    10981 aagcggtcgt ccggaccagc caacccgcac tcgtcgtcgc cgggcttcat gtcccagtcg
    11041 gcctcgggct cgaaatcctc gagaacctgt gcccaccact gggtttgggc gtagggatcg
    11101 tgggcgtcga tgcagaagtt ggatgcgcga gatgccatgc gacccatcgt gccgtttcac
    11161 cgccgcttcg tcacgtctta ctggctggac gaatgacgcg ggcggacgat gcagggtgag
    11221 acgcgagcgg gtgcctccgg cggccgtggg aggatcgacg gtggaaagca tttttccgac
    11281 ggcacccgca caaaggagca gatagtgccc atcgcaaccc ccgaggtcta ccgcgacatg
    11341 ctcgaccggg ccaaggatgg ctcgttcgca tacccggcga tcaatatcac ctcgagctcg
    11401 accatcaacg cggccatccg cggcttcgcc gaggccggct cggacggcat catccaggtc
    11461 tcgaccggtg gcgccgagtt cgcgtccggt tcgaacatca agaacatggt caccggctcg
    11521 ctggcactcg ccgcgttcgc cgaagaggtc gcgaagaact acccggtcaa catcgccctg
    11581 cacaccgacc actgccccga gcccaagctc gacggtttcg tgcgcccgct gatcgaggcc
    11641 tccgccgcgc gcgtcaaggc cggcgggctg ccgatcttcc agtcgcacat gtgggacggc
    11701 tcggccgtgc cgctggacca gaacctgcag atcgcccagg agctgctgga gaagtgccag
    11761 gcggccaaca tcatcctcga gatcgaggtc ggcgtcgtcg gcggcgaaga ggacggcgtc
    11821 gccaacgaga tcaacgacaa gctctactcc acgcccgagg acgccgtcgc gaccgtcaag
    11881 gcgctcggcg cgggggagaa gggctactac atgaccgccc tgaccttcgg caacgtgcac
    11941 ggcgtctaca agccgggcaa tgtcaagctg cgccccgacg tgctcaaggc cgcccaggaa
    12001 gccgccgccg gcgagctcgg tctggccgcg gacgccaagc ccttccacct ggtcttccac
    12061 ggcggctccg gctcgctgcc cgaggagatc tcggccgcgg tcgactacgg cgtcgtgaag
    12121 atgaacg
//
LOCUS       DAOPJG010000098        12062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5546_length_12062_cov_7.278587, whole genome shotgun sequence.
ACCESSION   DAOPJG010000098 DAOPJG010000000
VERSION     DAOPJG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5546_length_12062_cov_7.278587"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..636
                     /locus_tag="PKU75_09945"
     CDS             82..636
                     /locus_tag="PKU75_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HPF81242.1"
                     /translation="MAKPADLHRMGADEALAALYAAQWAPMVRLAWLLVRDQGVAEDV
                     VQDALLGIHRRWGQLADKGQAVGYLRRSVVNACRSVQRHRKVESDYLAKAGPAGRDAT
                     TASAEELALRQVGNAAMLAAVERLPRRQREVLILRYYADLSEAQIADTLQISPGSVKA
                     HAHRGLLALRDLVTDPRPSREDTP"
     gene            633..1703
                     /locus_tag="PKU75_09950"
     CDS             633..1703
                     /locus_tag="PKU75_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81243.1"
                     /translation="MNAPTDDLRDLEDSLRAALRADADAITPGDRLGEVRAAAMRQPG
                     QTRRARWLTVLAVAAAVVLVSTLAMASLRAGKPAVDPAASAVTLSSAPAGSKTSAGAA
                     TGASEATGAALPVYYLTKDPFRDLLRLDRTFVAPGDLEPPLAADASVTDRIAAALAWP
                     EPGVHAGEGIPTPTADMTVTGVDVSSDLITIGLGGVSGNESGWDETTTSRALQQIVLT
                     AQAVLGRGDIPVTFELADGATELWGRYAATERYHRPSTPLVEAMADPIWIDRPGYGQT
                     VPAGSPLTVSGLTAETSGRLFWQVDDQPWQEVATGGPAPDGAFLPVAYSFAVPALPSG
                     PHTLQVRTADERDVSSTRFVVE"
     gene            complement(1707..2450)
                     /locus_tag="PKU75_09955"
     CDS             complement(1707..2450)
                     /locus_tag="PKU75_09955"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPF81244.1"
                     /translation="MTLPLFLTDPGDLADARAGSAYVLGGAEGRHAATVKRLRAGEQL
                     MVGDGAGRQITGSVIEAAGNEVTMSVEQVTDEPPPVPSITLVQALAKGERDEAAIEAA
                     TEYGVDRIVPWQAERSIVQWRGERAEKSRSKWVDVVTAAAKQSRRAFVPPVEQLVDTS
                     QLPRRIGQATVAFILHEEAVLPLAAQPIPGAGEIIIIVGPEGGISPGELATLSSAGGI
                     PVRLGSTVLRSSSAGPAAIAVLSADERWR"
     gene            complement(2469..3599)
                     /gene="dnaJ"
                     /locus_tag="PKU75_09960"
     CDS             complement(2469..3599)
                     /gene="dnaJ"
                     /locus_tag="PKU75_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPF81245.1"
                     /translation="MNDYYQDLGVSRDASPEAIKSAYRRLARKLHPDVNPGHEAEEEF
                     KRVSQAYDVLSDPDKRRQYDMGNDPYGGAHAGFGQGFSFSDIMDAFFGPGAGGASSRG
                     PRSRTSRGQDGLVRLPIDLVDAVFGGEKDLTIETAAACGTCHGDGSQPGTGKRRCEVC
                     GGRGEVQQVQRSFLGQVMTSRPCGACQGFGETITHPCFECSGQGRVRTQRTMTIAIPA
                     GVDTGTRIHLVGEGEVGPGGGPPGDLYVEVSVNPHPTFTRRGDDLHASLQVPMTAAAL
                     GAGMKLETFDGVQDIDIKPGTQPGETITLRGLGVTHLRAGGRGDLLVHTDVRTPTKLD
                     AEQEGLLRQLAALRHEEAPTGKMSAPGDHGFMGKLRDAFKPR"
     gene            complement(3637..4710)
                     /gene="hrcA"
                     /locus_tag="PKU75_09965"
     CDS             complement(3637..4710)
                     /gene="hrcA"
                     /locus_tag="PKU75_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPF81246.1"
                     /translation="MRECQREEAHEMSSERHTSGDRRLAVLGAIVRDYVHTSEPVGSK
                     ALLERHAFGVSAATIRNDMAALEEEGLIVAPHTSAGRVPTDAGYRLFVDRLSQIKPMS
                     RAEQAAIAEFLQDPVDLDDVVDRTVRLLSSLTGQVAMMQYPSLTRSSVRHIEIVPIVD
                     SRLMVVLITTTGRVEQRILDIDTPLADEADLVARLRTGINSAAAGRLLVDAAAALSAL
                     PDAFAPGERDIVRHVVGALDDALVEEREERVTLAGTANLVRRPADFPATLEPVLDALE
                     QHVVLLRLLSTAAGGEHIAVRIGHENPVTGLQSTSMVTTGYGTGDQVVGGLGVVGPTR
                     MDYPASMAAVRAVAAYISSLLAT"
     gene            4772..5638
                     /locus_tag="PKU75_09970"
     CDS             4772..5638
                     /locus_tag="PKU75_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 domain-containing protein"
                     /protein_id="HPF81247.1"
                     /translation="MSDRYGSDVLSGDWRKPARGRAIDQPAEPGLVVEEVETGWVGAI
                     VGVETIGGQQVVRLEDRRGTVRAFPLGPGYLLDGAPVRLVPYAAPTAPRGPARTASGS
                     LAVAGVRAKVAAASRLFVEGTHDAELVEKVWGADLRVEGVVVEVLHGVDDLTAALREF
                     GPTPRARAGILVDHLVAGTKESRIVADTVAHFGADRVLVVGHPYVDVWQAIRPDRVGL
                     TAWPVIPRGQDWKAGIAAALGLPHTTAEDIGLAWKHVLSRVRSYADLEPAFSGRVEEL
                     IDFVTSPGEGAP"
     gene            5768..6349
                     /locus_tag="PKU75_09975"
     CDS             5768..6349
                     /locus_tag="PKU75_09975"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPF81248.1"
                     /translation="MTQQPNEPVYPGQQPAHPTQPFPQQSGPGQPLPPQQYPPQHYPP
                     QGPSAGSSSYSAPHQSPFGQTADRYTGSAPLTARDERNLGMLSHVIPLVCFVLSAGTL
                     GFVASLVMWFMYKDRGPFVRANAANSLNVQLTALIWSIISVIMMISIILLPVGIIGAI
                     VAVVYLFVVHIVAAMKASAGEWYKPPFTMSFFK"
     gene            complement(6411..7643)
                     /gene="hemW"
                     /locus_tag="PKU75_09980"
     CDS             complement(6411..7643)
                     /gene="hemW"
                     /locus_tag="PKU75_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPF81249.1"
                     /translation="MPPSALPDGDPAPVDGRLPPAALAARDAGAISAYVHVPFCRVRC
                     GYCDFNTYAPGELTGPAGSATAYGTAVEREIALAGEVLQDAGNLSLATVFVGGGTPTL
                     LPSEQLTGIVRRLASQWQLEDDAEVTTEANPDSVTIDSLATLRAGGFTRVSFGMQSSV
                     PHVLATLERTHDPANVAAAVAGARTAGLDVSLDLIYGTPGESLEDWRRSLEAAIALEP
                     DHISAYALVVEAGTKLAAQVRRGLITMPDDDDEADKYELADELLTAAGYGWYEISNWA
                     RPGKECRHNLAYWRGGDWWGFGPGAHSHVGGVRWWNVKHPGAYADRLVRGASPAAARE
                     DLTAEQRYDEQVLLAIRLAEGLPLDVLTAAGRAAVPALVDDGLLTDEPADAGRPRRVV
                     LTLRGRLLTDTVVRALLR"
     gene            8106..8876
                     /locus_tag="PKU75_09985"
     CDS             8106..8876
                     /locus_tag="PKU75_09985"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HPF81250.1"
                     /translation="MLQRWIELDGVVNMRDLGGLPTTDGGVVQPHRLIRSDNLQDLSP
                     ADVAHLVDLGVSDIVDLRSHREVHLTGEGPLRATTLRHHHHSFISEPLPGQDDPTFLS
                     TRPEHLATVKDQDYWSQHYRSYLVERPDSVSAALSVVATAQGATIIHCAAGKDRTGTV
                     VGLALAVAGVPRAEIVSDYVLTSERLDRIIDRLSGVDPYRETLRDKPRDEQIPLATSM
                     DALLTMVDDDFGGAAGWLRAQGWTDDEIDALRRKLTVE"
     gene            complement(8980..11844)
                     /locus_tag="PKU75_09990"
     CDS             complement(8980..11844)
                     /locus_tag="PKU75_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPF81251.1"
                     /translation="MSVTTAGAGSGLGSWRGSWAVALRMARREVRRHRGRSLLALSMV
                     AIPTALLTFALTLYPTSLITGPERIPYELGNAVAVLTTPGSDQIVQDPEAIQTMPAGF
                     SATGPGTTTRSAQPLPGLAKGSTPTPGQLATAMAAQFGGRVVPTTQLQVWILDGDRSR
                     RVDLVATPDPEAKGGLDLVSGRWASGPEEVVVTQTAVDKGLVPTSGTLLLDSTPARTL
                     RIVGVAQARSSWGAPLAGLIAPDTLGEQVDSYMTSYALYRDQPFTWPQVQQANTFGIS
                     ALSADVLRNPPPSGEIDPDLRDMMSMDDGRNAGLVVAGGAILLIIVTLLVGPAFAVSA
                     ARQRRTLALAASNGATTKQLRRTVLSQAVVLGALAAVAGAVIGVLAVPAVTHWIIPVW
                     TPWTGGPLDIPWTHVLGIMAVAMLSALVAALVPAQRLGRLDIVGVMKGQEVSPPPSRV
                     VFLLGLAGAILGGIVLFYGIITHASEVFIVGGAVLLIGGALLLVPTLLGLAALGSQHL
                     PVSLRLASRDAARQRSRSTPSVAAVVGAVAALTMMGVGLTSDTEQQRREYVPNALDGE
                     AIVSLDVYGSDGAPRRVDDVVARTTETIKRTTPGLVVVPLAQVTGGHVFAGPGSDPKA
                     VWPFVNYRPQGCTTTESISGDPDAPAVDGVPKCTQFGTHAWSGHGQIGVLPLAELDRR
                     FHLTDNERTVLDKGGALAFTRAALPASITMVSGTLRVDSDTGEFTDITERAADTLPLA
                     TRTLDLAALPALPRDCGLFVTPDTATALGWATTTDAIGLYDPAGPISSADADRLNAAM
                     TDSSYVEVERGFQRADQLIMGILVGIFAFLILVITLTSTALSMAEQQRDDATLAAVGA
                     TRGTRRAMAAGQAIVISLLGALIGVAVGLVPGIAITFPLTGNTQCDRLTNMCQEGYAH
                     PIIKIPWLWLGLLVVAVPLTAALVSALAVRRAPTMTRRAT"
BASE COUNT         1790 a         4238 c         4279 g         1755 t
ORIGIN      
        1 aaggtggccc gacaacgtgg ccgagctgcc ggtcaaccag atccgtgcgt gatacgtgga
       61 tggcgcgtga gaatggcggc cgtggcgaag cctgcagatc tacaccggat gggtgccgac
      121 gaggcgctcg ccgcgctgta tgccgcgcaa tgggccccga tggtgcgcct ggcctggctg
      181 ctcgtgcgcg accagggggt ggcggaggac gtggttcagg acgcgctgct gggcattcac
      241 cggcgctggg ggcagctcgc cgacaagggg caggcggtcg gctacctgcg ccgcagcgtc
      301 gtcaatgcgt gccgttcggt gcagcggcac cgcaaggtcg agtcggacta tctcgcgaaa
      361 gccggccccg ccggacgcga cgccaccacc gccagcgccg aggagctcgc cctgcgccag
      421 gtgggcaacg ccgccatgct cgccgcggtg gagcgcctcc cccgccgaca gcgcgaggtg
      481 ctcatcctgc gctactacgc cgacctgtcc gaagcccaga tcgccgacac cctccagatc
      541 tcgcccggct cggtcaaggc ccacgcccac cgcggcctgc tcgccctgcg cgacctggtc
      601 accgaccccc gcccctccag ggaggacacc ccatgaacgc ccccaccgac gacctgcgcg
      661 acctcgagga ttcgctgcgc gcggccttgc gggccgacgc ggacgcgatc accccgggcg
      721 atcgcctcgg ggaggtccgg gcagcggcga tgcgccagcc cggccagaca cgccgcgccc
      781 gctggctgac cgtgcttgcg gtcgcggccg cggtcgtgct ggtctccacc ctcgcgatgg
      841 cttcgctgcg ggcgggcaag cccgcggtcg atcccgccgc ctcggccgtg accctctcct
      901 cggcccccgc ggggtcgaag acgtcggcgg gagcggccac cggggcgtcg gaggcgaccg
      961 gtgccgccct tccggtctac tacctgacca aagacccatt cagggacctg ctccgactcg
     1021 accgcacctt cgtggcgccg ggcgacctcg agccccccct cgccgccgac gcgtccgtca
     1081 ccgaccgcat tgcggccgcg ctggcctggc cggagccggg tgtccatgcc ggtgagggca
     1141 tcccgacgcc caccgccgac atgaccgtga cgggagtgga cgtcagttcg gacctcatca
     1201 cgatcggcct cgggggcgtc tcgggcaacg agagcggctg ggacgagacg acgacctcgc
     1261 gcgcgctgca gcagatcgtg ctgaccgcgc aggcggtgct cggccgtggt gacatccccg
     1321 tgaccttcga actggccgac ggcgccaccg aactgtgggg ccgctatgca gccaccgagc
     1381 gctaccaccg gccgagcacc ccactcgtgg aagcgatggc cgaccccatc tggatcgacc
     1441 ggccgggcta cggccagacg gtgccggcag ggagtccgct caccgtgtcg gggctgacgg
     1501 ccgagacgag cgggcggctc ttctggcagg tcgacgacca gccgtggcag gaggtcgcga
     1561 cgggcgggcc ggcccctgac ggcgccttct tgccggtcgc ctactcgttc gccgtgcccg
     1621 cgctgccctc cggtccgcat acgctgcagg tgcggaccgc ggacgagcgg gacgtcagct
     1681 ccacccggtt cgtcgtggag taaggctcag cgccagcgct cgtccgcgga cagcaccgcg
     1741 atcgcggcgg ggccggcgga ggaggaccgc aggacggtcg agccgaggcg cacggggatg
     1801 ccgccggcgg aactgagcgt cgcgagttcg ccaggcgaga tgccaccctc cgggccgacg
     1861 atgatgatga tctcgccggc acccggaatc ggttgtgccg caaggggaag cacggcctcc
     1921 tcgtgcagga tgaaggcgac cgtggcctgc ccgatccggc gcggcagctg ggaggtgtcg
     1981 accagttgct cgaccggcgg cacgaacgcg cggcggctct gcttggccgc cgccgtgacc
     2041 acgtcgaccc acttggagcg cgacttctcg gcgcgttcac cacgccactg cacgatcgat
     2101 cgctcggcct gccagggcac gatgcggtcg acgccgtact cggtggcggc ctcgatggcc
     2161 gcctcgtccc gctcaccctt ggccagggcc tgcaccagcg tgatcgacgg cacggggggc
     2221 ggctcgtcgg tgacctgctc gacgctcatc gtgacctcgt tgcccgcggc ctcgatgacg
     2281 gagccggtga tctggcgccc ggcgccgtcg ccgaccatca gctgctcacc cgcccgcagg
     2341 cgcttgacgg tggcggcatg ccggccctcg gcgccgccga ggacataggc cgagccggcc
     2401 cgcgcgtccg cgagatcgcc ggggtcggtc aggaagagcg ggagggtcat cggatcacca
     2461 gatcggggtc agcggggttt gaaggcatca cgcagcttgc ccatgaagcc gtggtcgccc
     2521 ggagcggaca ttttgcccgt gggtgcttcc tcgtgccgca gcgcggccag ctgacgcagc
     2581 agcccctcct gctcggcgtc gagcttggtc ggagtgcgca cgtcagtatg cacgagcagg
     2641 tcgccccgtc cgcccgcgcg caggtgggtc acgccgagac cgcgcagggt gatcgtctcc
     2701 cccggctgcg tgcccggctt gatgtcgatg tcctggacgc cgtcgaaggt ctccagtttc
     2761 atcccggcgc cgagcgcggc cgccgtcatc ggcacctgca ggctcgcgtg caggtcgtcg
     2821 ccgcgccggg tgaaggtggg gtgcgggttg accgacacct cgacatacag atccccgggg
     2881 ggaccaccac ccggcccgac ctcgccctcc ccgacgaggt ggatccgggt gccggtgtcg
     2941 acaccggccg ggatggcgat cgtcatcgtg cgctgagtgc gcacccggcc ctgccccgaa
     3001 cactcgaagc acgggtgggt gatcgtctcg ccgaagccct ggcacgcgcc gcaggggcgc
     3061 gacgtcatga cctgcccgag gaagctgcgc tggacctgct gcacctcgcc gcggccaccg
     3121 catacctcgc agcggcgctt gccggtgccc ggctgcgacc cgtcgccatg acacgtgccg
     3181 cacgcggcag cggtctcgat ggtcaggtcc ttctcgccgc cgaagacggc gtccacgagg
     3241 tcgatcggca gccgcaccag gccgtcctga cctcgcgagg tgcgcgagcg cgggccccgc
     3301 gacgacgcgc cgccagcacc gggcccgaag aaggcgtcca tgatgtcgct gaacgagaag
     3361 ccctgcccga aaccggcgtg cgccccgccg tacgggtcgt tgcccatgtc gtactggcgc
     3421 cgcttgtcgg ggtcggagag cacgtcatac gcctgcgaga cgcgcttgaa ctcctcctcg
     3481 gcctcgtggc cggggttgac gtccgggtgc agcttgcggg cgagccggcg gtatgccgat
     3541 ttgatcgcct cggggctggc atcccgggag accccgagat cctggtagta gtcgttcacc
     3601 tgatcctctg atcgattcgg tcgagttcgc gccgggctag gtggccagga gcgaggagat
     3661 gtaagcggcg acggcgcgga ccgcggccat gctggcgggg tagtccatcc gggtcggacc
     3721 gaccacgccg agcccgccga cgacctggtc gcccgtgccg tagccggtgg tcaccatcga
     3781 ggtcgactgc agccccgtga ccgggttctc gtggccgatg cgcacggcga tgtgctcgcc
     3841 accggccgct gtggagagca accgaagcag caccacatgt tgttccaggg cgtcgagcac
     3901 gggctccagg gtggcgggga agtcggccgg ccggcgcacc agattggccg tgccggcgag
     3961 ggtgacccgc tcctcgcgct cctcgacgag agcgtcgtcg agtgccccga cgacatggcg
     4021 cacgatgtcc cgctcccccg gcgcgaacgc atcgggcagg gcggacaggg ccgccgcagc
     4081 atcgacgagg agccggccgg cggcggcgga gttgatgccg gtgcgcaggc gcgcgacgag
     4141 gtcggcctcg tccgcgagcg gggtgtcgat gtcgaggatg cgctgttcga cccgtccggt
     4201 ggtcgtgatg aggacgacca tcaggcggga atcgacgatc gggacgatct cgatgtgccg
     4261 cacgctcgag cgggtcagcg acgggtactg catcatcgcc acctgcccgg tcagcgacga
     4321 caacaaccgc accgtgcgat cgacgacgtc gtcgaggtcg acggggtcct gcaggaactc
     4381 ggcgatcgcg gcctgctcgg cccggctcat cggtttgatc tgggagagcc ggtcgacgaa
     4441 aagccggtag ccggcgtccg tcggcactcg ccccgcgctc gtgtgcggcg cgacgatcag
     4501 cccctcctcc tcgagggcgg ccatgtcgtt gcggatggtg gccgccgaca cgccgaacgc
     4561 gtgccgctcc aggagcgcct tggacccgac cggctcgctg gtgtgcacat agtcgcggac
     4621 gatggccccg agcacggcga ggcggcgatc gccggacgta tgccgttcgc tggacatctc
     4681 gtgggcctcc tcccgctggc actcgcgcat acagagtgcc aattctatct acctcccgga
     4741 gtccgctcga ctccgcgcat aaagtgcccg ggtgagcgat cggtatggca gtgacgtgct
     4801 ctccggcgac tggcggaaac cggcgcgggg ccgggcgatc gatcagcccg cggaaccggg
     4861 cctggtcgtc gaggaggtag agaccggctg ggtgggggcg atcgtcggcg tcgagacgat
     4921 cggcggccag caggtggtcc ggttggagga ccgccgcggc accgtccgag ccttcccgtt
     4981 ggggccgggt tatctgctcg acggggcgcc ggtccggctc gtgccgtatg ccgcgcccac
     5041 cgccccgcgc ggtcccgccc ggaccgcgag tggctcgctc gcggtggccg gggtgcgcgc
     5101 gaaggtcgcc gccgcctcgc ggctgttcgt cgagggcacc cacgacgccg aactcgtcga
     5161 gaaggtctgg ggcgccgacc tgcgggtcga gggagtggtc gtcgaggtcc tgcacggcgt
     5221 ggacgacctg acggcggccc tgcgcgaatt cggccccacg ccgagggccc gcgccgggat
     5281 cctcgtcgac catctcgtcg ccggcacgaa ggagagccgg atcgtcgccg acaccgtcgc
     5341 gcatttcggc gctgaccggg tgctcgtggt cgggcacccg tatgtcgatg tctggcaagc
     5401 gatccggccc gaccgggtgg gcctgacggc gtggccggtc atcccccgcg gccaggactg
     5461 gaaggcgggc atcgccgcgg ccctggggct gccgcatacg accgcggagg acatcggcct
     5521 ggcctggaaa cacgtgcttt cgcgggtgcg gtcgtatgcc gacctggagc ccgcgttctc
     5581 cgggcgcgtg gaggaactca tcgacttcgt cacctcacct ggtgagggtg cgccctgact
     5641 ctcccccgag gtggccctcg acttggcgcc gaatggaatg cagcacgccg attgtgcggt
     5701 tgactagtca cgacggcaca gtccgggaag gtcccgagcc ctgccgccac aaggggaaga
     5761 aacagccatg acgcagcagc ccaacgagcc ggtctacccg ggccagcagc cggcgcaccc
     5821 gacccagccg ttcccgcaac agtccggacc gggccagccc ctgccgccgc agcagtatcc
     5881 gccgcagcac tatccgccgc agggcccgtc ggctggcagc agcagctact cggctccgca
     5941 ccagagcccg ttcggccaga ccgcggaccg ctacaccggc tccgccccgc tcaccgcgcg
     6001 cgacgagcgc aacctcggca tgctctccca cgtcatcccg ctggtctgct tcgtgctcag
     6061 cgccggcacc ctcgggttcg tcgcctctct ggtcatgtgg ttcatgtaca aggaccgcgg
     6121 cccgttcgtg cgcgcgaacg ccgcgaactc cctcaacgtc cagctcaccg ctctgatctg
     6181 gtcgatcatc tccgtcatca tgatgatctc gatcatcctg ttgccggtgg gcatcatcgg
     6241 cgccatcgtc gctgtcgtct acctcttcgt cgtgcacatc gtcgcggcga tgaaggcgag
     6301 cgccggcgag tggtacaagc cgcccttcac catgagcttc ttcaagtagc cccacgacgt
     6361 gtcctcgctc ctgcgtcgct gcaggacttc gcgagccgac cgacgggttc ctaccgcagc
     6421 agggcacgca ccacggtgtc cgtgagcagc cgcccccgca gggtcaacac gacccggcgg
     6481 gggcgtcctg cgtccgccgg ctcgtcggtg agcaggccgt cgtcgacgag cgccggcacg
     6541 gcggcccggc cggccgcggt gagcacgtcg agcggcagtc cctcggcgag gcggatcgcg
     6601 aggaggacct gctcgtcata ccgctgctcg gccgtgaggt cctcgcgggc ggcggccggc
     6661 gaggcacccc ggacgagccg atcggcatac gcaccagggt gtttcacgtt ccaccagcgc
     6721 acgccgccga cgtggctgtg ggctcccggg ccgaaccccc accagtcgcc gccgcgccag
     6781 taggcgaggt tgtggcggca ttccttgccg gggcgggccc agttgctgat ctcgtaccac
     6841 ccatagcccg ccgcggtgag cagttcgtcg gcgagttcgt atttgtcggc ctcgtcatcg
     6901 tcgtcgggca tggtgatcag gccgcgccgc acctgcgccg ccagcttcgt gcccgcctcg
     6961 acgacgaggg cgtatgccga gatgtggtcc ggctccaggg caatcgccgc ctccagactg
     7021 cggcgccagt cctcgagcga ctcccccggc gtgccgtaga tgaggtcgag actgacatcg
     7081 agcccggcgg tcctcgcgcc ggcgacggcg gcggcgacat tggcggggtc atgggtgcgc
     7141 tccagtgtcg ccagcacgtg gggcacgctc gattgcatcc cgaagctgac ccgggtgaag
     7201 ccaccggcgc gcagggtggc caggctgtcg atggtgacgg aatcggggtt cgcctcggtg
     7261 gtgacctcgg cgtcgtcctc gagctgccac tgggacgcca gtcgtcgcac gatccccgtc
     7321 agttgctccg acggcagcag cgtcggcgtg cctcctccga cgaaaaccgt tgcgagactg
     7381 aggttccccg catcttggag cacctcgccg gccagcgcga tctcgcgctc cactgcggtg
     7441 ccgtatgccg tggccgagcc cgccggaccc gtcagctcgc cgggggcgta ggtgttgaag
     7501 tcgcagtagc cgcagcgcac gcggcaaaag ggcacgtgca cataggcgga gatcgccccc
     7561 gcgtcgcggg cggccagcgc cgcgggcggc aggcggccgt cgacgggcgc gggatccccg
     7621 tcggggaggg cggagggcgg catacccgcg atcctttcag agcgctgcgg ggacacccgc
     7681 atcacacgcg ccacagcgag gacggcgtgt ccagcacgac acgccgagga cacgaaaaaa
     7741 ggtgaaaaaa ggcggcggct aagcgttgcg cagacccctc ggacgggata gaaatgacgt
     7801 gtcgcagtca cctcggtgac agcgacacag atcaggtctt cggacctgat cacgacacga
     7861 aattcgtttc gtgtcaccag gtttcgactt cggtcgaaac cacccgagat cgacttcggt
     7921 cgacctcgga gagggcctcg gaaactcata cttcccgagg ccctcactta tgtcccgggg
     7981 cttcggtgtc ccccgtgctc cagtggctcc cgtgctccac ggtgctccgg tgtcctcggg
     8041 gctccggtcg ttccgcgcca tccgccgttc atcgctgtgt cacgagcacg gcttagcctg
     8101 tcggggtgct gcagcggtgg atcgaactgg acggcgtcgt gaacatgcgg gacctcggcg
     8161 gccttccgac cacggacggc ggggtcgtgc agccgcaccg gctcatccgg tccgacaatc
     8221 tgcaggatct ctcccccgcc gatgtcgcgc acctggtcga tctcggggtc agcgatatcg
     8281 tcgatctgcg ctcacaccgg gaggtgcacc tgaccggaga gggtccactg cgggccacca
     8341 cgctgcgcca ccaccaccac agtttcatca gcgaacccct tccaggacag gacgatccga
     8401 ctttcctgtc gacccggccg gaacacctgg cgacggtcaa ggaccaggac tactggagcc
     8461 agcactaccg gagctatctc gtcgaacgcc ccgactcggt ctcggccgcg ttgtccgtcg
     8521 tcgccaccgc gcagggtgcg acgatcatcc actgcgccgc cggcaaggac cgcaccggca
     8581 ccgtcgtcgg gctggccctc gccgtcgccg gcgtgccgcg ggcggagatc gtctccgatt
     8641 acgtgctgac gagcgagcgg ctcgaccgga tcatcgaccg gctgagcgga gtcgacccct
     8701 atcgagaaac gttgcgcgac aagccacgcg atgagcagat cccactcgcg acgtccatgg
     8761 acgcactatt gaccatggtg gacgacgact tcggcggcgc cgccggatgg ctgcgtgccc
     8821 aggggtggac ggacgatgag atcgacgcgc tccggcgcaa gttgacagtc gagtgagcgc
     8881 gacgcgccca cgtccagcgc cacacgtggg cgtgtcgcgc tcactcgact cgggtgccac
     8941 ggcgcaggtg tggggccggg gtcgggtgtg gggccagggt caggtcgcgc ggcgggtcat
     9001 cgtgggggcg cgccggacgg cgagggccga cacgagggcc gcggtgagcg ggacggcgac
     9061 cacgaggagt cccagccaca gccacgggat cttgatgatc gggtgggcgt agccctcctg
     9121 acacatattc gtcaggcggt cgcactgggt gttgcccgtg agcgggaagg tgatcgcgat
     9181 cccggggacc agcccgacgg ccaccccgat gagcgcgccg agcaggctga tgacgatggc
     9241 ctgcccggcg gccatcgccc gccgggtgcc gcgggtcgcc ccgaccgcgg ccagtgtggc
     9301 gtcgtcacgc tgctgttcgg ccatcgacaa ggcggtcgag gtcaaggtga tgacgaggat
     9361 gaggaaggcg aagatgccga cgaggatccc catgatcagc tggtccgcgc gctggaagcc
     9421 gcgctcgacc tcgacatagg aggagtccgt catggcggca ttcagtcgat ccgcgtccgc
     9481 gctgctgatc gggcccgcgg ggtcgtagag gccgatggcg tcagtggtgg tggcccagcc
     9541 cagcgccgtg gcagtgtccg gagtgacgaa gaggccgcaa tcgcggggca gtgccggcag
     9601 cgccgcgagg tcgagcgttc gggtggccaa tggcagcgtg tcggctgccc gctccgtgat
     9661 gtccgtgaat tccccggtgt cggagtcgac gcgcaaggtg ccgctgacca tggtgatcga
     9721 ggcgggaagg gcggcgcgcg tgaacgcgag ggccccaccc ttatcgagca ccgtgcgctc
     9781 gttgtcggtc aggtggaagc ggcgatccaa ctcggccagc gggagcacgc cgatctggcc
     9841 gtgcccggac cacgcgtggg tgccgaactg ggtgcacttg gggacgccat ccaccgccgg
     9901 ggcatccgga tcccccgaga tggattcggt ggtcgtgcag ccttgcggcc gatagttgac
     9961 gaacggccag accgccttcg ggtcggaacc cgggccggcg aagacgtggc cgccggtgac
    10021 ctgcgccagc ggcaccacga ccaggccggg cgtggtgcgt ttgatcgtct ccgtcgtgcg
    10081 cgccaccacg tcgtctacgc gtcgtggggc gccgtcggag ccatacacgt cgagggagac
    10141 gatggcctcg ccgtccaggg cgttcggcac atattcgcgg cgctgctgtt cggtgtcgct
    10201 ggtcagaccg acgcccatca tcgtcagcgc ggccacggcg ccgacgacgg cggcgaccga
    10261 gggcgtcgat cgcgaccgct ggcgagcggc atcgcggctg gccagccgca aggacacggg
    10321 caagtgctgg gagccgagag cggcgagtcc gagcagggtg ggcaccaaca gcaacgcgcc
    10381 gccgatgagc aacacggcac cgccgacgat gaagacctcg gaggcgtgag tgatgatgcc
    10441 gtagaacagg acgatccccc cgaggatcgc gccggccaga cccagcagga agaccacgcg
    10501 ggacggcggc ggcgagacct cctgcccctt catcaccccg acgatgtcga gccgtcccag
    10561 tcgttgcgcc gggaccaggg ccgcgacaag cgcagagagc atggcgacgg ccatgatccc
    10621 gagcacgtga gtccacggga tgtcgagggg cccgccggtc cacggcgtcc agaccgggat
    10681 gatccagtgc gtgacggccg gcaccgcgag caccccgatc acggctccgg cgacagcggc
    10741 gagtgccccg aggacgacgg cctgggagag cacggtccgg cgcaactgct tggtggtcgc
    10801 cccattgctc gcggccagcg ccagcgtccg tcgctggcgc gccgcgctca cggcgaatgc
    10861 cggcccgacc agcagggtga cgatgatcag caggatcgcg ccgcccgcga cgacgaggcc
    10921 ggcattgcgg ccgtcgtcca tggacatcat gtcgcgcaaa tcggggtcga tctcgccact
    10981 cgggggtgga ttgcgcagca cgtcggccga cagggcggag atgccgaatg tgttggcctg
    11041 ctgcacctgc ggccaggtga acggctgatc tcggtagagc gcgtagctcg tcatatagct
    11101 gtcgacctgc tccccgaggg tgtccggggc gatgaggccg gccaggggtg cgccccagga
    11161 actgcgcgcc tgcgcgacac cgacgattcg caaggtccgc gccggtgtcg agtccaggag
    11221 cagcgtgcca ctcgtgggca ccaggccctt gtcgacggca gtttgggtga ccaccacctc
    11281 ctccggcccc gacgcccagc gcccggacac caagtcgaga ccgcctttcg cctcgggatc
    11341 gggcgtggcc accaggtcga cccggcgtga ccggtcaccg tcgagaatcc agacctgcag
    11401 ctgcgtcgtg ggaaccaccc ggccaccgaa ctgggccgcc atcgccgtgg ccagctgtcc
    11461 gggggtgggc gtgctgccct tcgccaggcc aggcagcggt tgggccgacc tggtcgtcgt
    11521 ccctggaccc gtggcagaga accctgccgg catcgtctgt atggcctctg ggtcctgcac
    11581 gatctgatcg ctgccgggcg tggtcaagac ggcgacggcg ttgccgagtt cgtacgggat
    11641 gcgctcgggg ccggtgatca ggctcgtcgg atagagcgtg agcgcgaagg tcaggagcgc
    11701 cgtcgggatc gcgaccatcg agagcgcgag caggctgcgt ccgcggtggc ggcggacctc
    11761 gcgccgcgcc atccgcaacg ccaccgccca cgagcctcgc caactgccga gcccgctgcc
    11821 ggctcctgcc gtggtcacac tcacgtcgcg ctcaacccct ccccatactc ggtatctacc
    11881 cagcacgcaa gcgtataccc ggtatggcgg atggtccagt caggctgcgg gtgtgtctcc
    11941 aacccgggcg agggcgtcgt cgcgcaacca ctgcgtggga ctggccccgg cgaactcgcg
    12001 ccagtctcgc accaggtgac tctggtcggc atacccacaa cggattgcga tgtccgccaa
    12061 cg
//
LOCUS       DAOPJG010000099        11830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5690_length_11830_cov_7.164756, whole genome shotgun sequence.
ACCESSION   DAOPJG010000099 DAOPJG010000000
VERSION     DAOPJG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5690_length_11830_cov_7.164756"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2509
                     /gene="ppdK"
                     /locus_tag="PKU75_09995"
     CDS             <1..2509
                     /gene="ppdK"
                     /locus_tag="PKU75_09995"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPF81252.1"
                     /translation="TEDQIGRHFGADKEPLLLSVRSGAKFSMPGMMETVLNVGLNDTS
                     VQGLAEFASDERFAWDSYRRLIQMFGKTVLDIDGELFAAALDAQKAKAGVASDPELTA
                     ADHKEVVAAYKQIVLDEAGLEFPQDPRTQLNMAIEAVFNSWNTERARLYRRRERIPND
                     LGTAVNVMAMVFGNLGETSGTGVCFTRDPSTGHSGVYGDYLSNAQGEDVVAGIRNTLP
                     LAELEKLDPKSYADLRAAMRQLETHYRDLCDIEFTVERGKLWMLQTRIGKRTAAAAFR
                     VATQLVDEHLITMDEALGRVSGDQLSQLLFPHFAADSGVTPLAKGLGASPGASVGEIV
                     FDNDGATARKEAGAKVILVRRETSPDDLPGMVASVGVLTARGGKTSHAAVVARGMGMC
                     AVVGAEALKVDPNAKTVTVGGTVLHEGEIIAIDGATGEIFVGEVPVVDSPVMVYISEG
                     IDAAQQAAHGDPETQDLINSVDRLLRYADGVRRLRVRANADTAEDAIRARERGAQGIG
                     LCRTEHQFLGERRTYVEAVILAETEDERTAALDPLVPVQRQDFVELLTAMDGLPTTIR
                     LIDPPLHEFLPDLTELSVKVAVAEAKGEEADPHELKLLAAVQRMHEANPMLGLRGVRL
                     GLKIPGLFDLQIRAIAEATVELLGQGKRPKPEIMVPLVGSVRELQIIRDRAEKIIADV
                     TATTGTALRIPIGCMIELPRAALTAGRIAEEAEFFSFGTNDLTQTTWGFSRDDVESAF
                     FADYLESGVLTISPFETLDGFGVGKLVKMGVEAGRATRPGIHVGVCGEHGGDPESIHF
                     FHAAGLDYVSCSPFRVPVARLEAGRAAVDASEHAASA"
     assembly_gap    2570..2669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2670..3290)
                     /locus_tag="PKU75_10000"
     CDS             complement(<2670..3290)
                     /locus_tag="PKU75_10000"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPF81253.1"
                     /translation="MRDPRTGVDVVAVEHGSDIDGAPWVVLDAGGGRWLADHVVVATG
                     YNHTPVAPSWPGQDGFTGEVIHASAYRNPGPFIGRSALVVGTGNTGCEIAQDLAEHAV
                     SPVWLSYRTAPHILRRTTGPVPQQWTGVAVRRLPPRVVDRLGAVVERISMPDLTAYGL
                     PRPQADLYSRVDDGSIPVQDVGIVAAIRNRSVLPVPTIERFEAAQVV"
     gene            complement(3423..4097)
                     /locus_tag="PKU75_10005"
     CDS             complement(3423..4097)
                     /locus_tag="PKU75_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPF81254.1"
                     /translation="MPALPLFPLATIAVPGGELPLVIFEPRYRALLADVLALPETERI
                     FGVVAIRRGHEVGAGRAEQLYDVGTSVRVVSVHPIPAGIRVGCVGVQRFRLDARVPGA
                     DYLVGEVTWLDEEPGDVDALPELVVQLREAWLTYADSVGAPALLDPPAPVAGGVDGDR
                     ARALAYAVLDQLRLPLAERQHVLEAVTTSERLQELVARVRREVGLAAALQARPLAPPS
                     VGPSLN"
     gene            complement(4109..5455)
                     /locus_tag="PKU75_10010"
     CDS             complement(4109..5455)
                     /locus_tag="PKU75_10010"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPF81255.1"
                     /translation="MTLAPDRTSISPTAPHVSEPTHVAAPHHTLNTRLSVSGGAERTH
                     PPKKSGEPNPMGHLTNEQIEQIGIELDAIRAEVMNSRGAKDAAYVRRIIKTQRTLEIG
                     SRALLLFSGVKVKGVRPAFWLGTAGLSLAKILENMELGHNILHGQWDWMRDPKIHSST
                     WDWDMASPARQWQHSHNQLHHTYTNVLGKDTDLGYGLLRVDKDQQWELFHLGQPIWHL
                     VNAMFFEYGIAAYDLELRGNLQNWDQLSDEDKATFKANLHEMLTKIRKQCTKDYVVHP
                     LLSGPNFGWTLLANFSANIVRNVWSNTVIICGHFPDDIQTFETDSIDGETRGEWYIRQ
                     MLGSGNISGPTLLHILSGNLSNQIEHHLFPDMPSNRYETISPQIRDLMQRNDLHYTSG
                     PLLKQAYQVYRKVCRLALPNEEPGRTRWSVVRGELGKVLKGQGGARTKRHAPSTAV"
     gene            complement(5487..6587)
                     /locus_tag="PKU75_10015"
     CDS             complement(5487..6587)
                     /locus_tag="PKU75_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HPF81256.1"
                     /translation="MTAAPPRPARGVLPALTSGVWKVAAAAATPIVPTEILDMIAPLR
                     AGAELRGRIVAIHRETDDAATVVIRPGKSWRGHIPGQYIRIGIDIDGVRHWRAYSLTS
                     RSDAADGTISITPKAILDGLVSNHLVRDARPGTLVMLDQAIGDFVLPATLPAKILFVT
                     GGSGITPVMGMLRNLPLHPAADIVLLHSAPQPQDVVFAAELAERAASGQIRLSVRHTD
                     VDGMLDFAGLDAEVPDWRERKVWVCGPTGLLDAAEMHWAAHELTDALHVERFRPTIIE
                     PGEGGEITLLDSELTIDADGSTPILDAAEDAGVLMTSGCRMGICFGCVVTMRQGAIRD
                     LRTGEITTVEPGDELPIQTCINAVAGSCDLEV"
     gene            6719..8950
                     /locus_tag="PKU75_10020"
     CDS             6719..8950
                     /locus_tag="PKU75_10020"
                     /inference="COORDINATES: protein motif:HMM:NF016047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HPF81257.1"
                     /translation="MRLRFRRRLPLVATALAVSAATAVTGGSPATASESSPAPAPGLR
                     SAAFPDKALNGHLVRPASELGAASAPKAGARRTSGARAAGGKYAGEHRIYYLPVSWTT
                     SEPSQDPITNADTYVGRADSYWQDVSGGSVDVTLGWADDWWRIHLSSAEVATCDRKAI
                     WREASALIEGGGGDFDHVLVNLPKNPACDWVDVEYFGPTSAGYGLTMTNGVYVPKDDV
                     INRAMMHNNWVLSTDQTMCKDSSGKPVPISGDCSYRLYVDPWSPLSDQPFGYTRTGFP
                     HARELLDFGVFGSDEWTTVTPGDLPQTVTLQRLQAPGNGTQVLGFTLDGSWYDIEYRA
                     AVGRDDWIDNKTYTDSSGVTRTTPGGGVTLVRTRIPVEQPDGSTLVWDGSLVDFHPRG
                     TSSSYVTERHPGLEPGESYTAPDGLFKISVISANLSSARVEISFPGLRKVARWSGKDR
                     YAASVEMSQQSFAPGVDRVLIASGEVYTDALSGAPVGGRDGDPILLTKADELPSVVEA
                     ELRRLDPASVVILGGPATISDRVASRLASVTGVTPQRWSGADRFVTSAQISERSYEAG
                     GVDTAYVASGRVFPDALSGAPASGKAGGPVLLVDTDELPSEIAAELRRLSPRRIVVLG
                     GTATIATGVETQLRGLASSVERWAGPDRYATAVEVTKHSYDPGVGTAYIASGLVFADA
                     LSGAPIAGMTKGPILLTDTAKLPSVVAAELERLKPKRIVVLGGANTITYAQQAQLGRY
                     VVS"
     gene            9009..9404
                     /locus_tag="PKU75_10025"
     CDS             9009..9404
                     /locus_tag="PKU75_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HPF81258.1"
                     /translation="MTTAGLVFAAAAALIHVYIFVLESLRFDQPATWKVFGVKGPEAA
                     EVIRPWAFNQGFYNLFLGLGAALGVFLCAFNRQGTTAYVRGAAIALFCCAVMVAAALV
                     LLVTDRRKARAALVQGLAPALAVAFLLVG"
     gene            complement(9539..10462)
                     /locus_tag="PKU75_10030"
     CDS             complement(9539..10462)
                     /locus_tag="PKU75_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF81259.1"
                     /translation="MTTTTVTAADGTALHTYVWTPQGQPKAIVQIAHGLAEHAQRYGR
                     FADALTAAGYAVYANDHRGHGHTNPVGEGHGYFADTDGFGKVVEDLRAVTEVARAAYP
                     GVPVVLLGHSMGSFLARAYAAAYGADLAGLILSGTAGDAGALGKVGIVVAQAQARLKG
                     RTHASGLLNAMSFGQYNKDFKPNRTDFDWLSRDPDEVDKYVADPLCGNVFSTGFWIDL
                     LGGLNYVHRRDTTAKVPKTLPVHLIAGSMDPVGAKTKGVEAVANQYRAAGVQQVTTHY
                     YIGARHELLNETNRDEVTADLIAWLDAHLPG"
     gene            10553..10825
                     /locus_tag="PKU75_10035"
     CDS             10553..10825
                     /locus_tag="PKU75_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81260.1"
                     /translation="MNAGDNTPTYGHSGDEAQDGQIRPIPVPSQGGASVPAERKGGTM
                     AGLMAISSTLALIAFMIIGFVFDGWAWGWVVFLMPGLLKSWQAAQN"
     gene            10900..11784
                     /gene="ppk2"
                     /locus_tag="PKU75_10040"
     CDS             10900..11784
                     /gene="ppk2"
                     /locus_tag="PKU75_10040"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HPF81261.1"
                     /translation="MNLREYIDSLKTGGYTVRDDHGDDPMLISPEGKAVETWRENYPY
                     DELMSRNDYEVEKYLLQIELLKFQYFVQDMGEKHVIVFEGRDAAGKGGTIKRFMEHLN
                     PRSARVVALTKPTTTEQGQWYFQRYVAHLPTAGEIVLFDRSWYNRAGVERAMGFCTED
                     QYQVFMRQAPRFEEMLIDAGATVTKLWFSVTQQEQRTRFAIRQIDPVRRWKLSPMDLE
                     SLDKWEAYTEAKEAMFKFTDTKHSQWHTIKSNDKKRARINAMRLFLNMHDYDGKDPEV
                     VFEPDPLIVGRGKKTIGD"
BASE COUNT         1863 a         3967 c         3997 g         1903 t
ORIGIN      
        1 gaccgaggac cagatcggcc gccacttcgg tgccgacaag gagcccttgc tgctgtccgt
       61 gcgctcgggc gccaagttct cgatgcccgg catgatggag accgtcctca acgtcggtct
      121 caacgacacc tcggtccagg gtcttgccga gttcgcgagc gacgaacgct tcgcctggga
      181 ctcctatcgg cgcctgatcc agatgttcgg caagaccgtc ctcgacatcg acggtgaact
      241 ctttgccgcg gcgctggacg cgcaaaaggc caaggccggc gtcgccagcg accccgagct
      301 gaccgctgcg gaccataagg aggtcgtcgc ggcctataag cagatcgtcc tggacgaggc
      361 gggcttggag ttcccgcagg atccccgcac ccagctcaat atggccatcg aggccgtctt
      421 caacagctgg aacaccgagc gggcccggct ctatcgccgc cgcgagcgca tccccaacga
      481 cctcggtacc gccgtcaatg tgatggcgat ggtcttcggc aacctcggcg agacctccgg
      541 caccggcgtc tgcttcaccc gtgacccctc caccggccac tccggcgtct atggggacta
      601 cttgtccaac gcccagggcg aggatgtcgt cgccggcatc cgcaatacgt tgccgctcgc
      661 cgagctggag aagctggacc cgaagtcgta tgccgatctg cgcgcggcca tgcgccagct
      721 cgagacccac tatcgcgacc tgtgcgacat cgagttcacc gtcgagcgcg gcaagctgtg
      781 gatgctgcag acccgcatcg gcaagcgcac cgccgccgct gctttccgcg tggcgaccca
      841 actcgtcgac gagcacctga tcaccatgga cgaggcgctc ggacgggtga gcggggatca
      901 gctcagccag ttgctcttcc cgcacttcgc cgccgactcc ggcgtcacgc cgctcgccaa
      961 gggcctcggc gcctcccccg gtgcttccgt cggtgagatc gtcttcgaca acgacggcgc
     1021 cacggcccgc aaggaggccg gggccaaggt catcctcgtc cgccgggaga cctcaccaga
     1081 cgatctgccg ggcatggtcg cctcggtggg cgtcctgact gcccgcggcg gcaagacctc
     1141 gcacgcggct gtcgtcgccc ggggcatggg catgtgcgcg gtcgtgggcg ccgaggcgct
     1201 caaggtggat ccgaacgcca agaccgtgac cgttggtggc acggtcctgc acgagggcga
     1261 gatcattgcc atcgacgggg ccaccggcga gatcttcgtc ggtgaggtgc cggtggtcga
     1321 ctcccccgtc atggtctata tctccgaggg catcgacgca gcccagcagg ccgcgcacgg
     1381 cgacccggag acccaggacc tcatcaactc cgtcgaccgg ctgctgcgct atgccgacgg
     1441 tgtccggcgg ctgcgcgtgc gcgccaatgc cgacaccgcc gaggacgcga tccgcgcccg
     1501 cgagcgcggc gcgcagggaa tcggcctgtg ccgcaccgaa caccagttcc tcggcgagcg
     1561 ccgcacctat gtcgaggcgg tcattctcgc cgagaccgag gacgagcgca cggccgcgct
     1621 ggacccgctg gtgccggtcc agcggcagga cttcgtcgaa ctgctcaccg cgatggacgg
     1681 cctgccgacg acgatccggc tgatcgaccc gccgctgcac gagttcctgc cggacttgac
     1741 cgagctgtcg gtcaaggttg ccgtcgccga ggccaagggc gaggaggccg atccccacga
     1801 gctgaagctg ctcgccgcgg tccagcgcat gcacgaggcc aacccgatgc tgggtctgcg
     1861 tggcgtgcgg ctcgggctga agatccccgg cctgttcgac ttgcagatcc gggcgatcgc
     1921 cgaggcgacg gtcgaactcc tcggccaggg caagcggccc aagccggaga tcatggtccc
     1981 gctcgtcggc tcggtgcgcg aactgcagat catccgcgac cgcgccgaga agatcatcgc
     2041 cgatgtcacg gcgaccactg gcaccgcctt gcggattccg atcgggtgca tgatcgagtt
     2101 gccgcgtgcc gccttgactg ccggcaggat cgccgaggag gccgagttct tctccttcgg
     2161 caccaacgac ctgacgcaga cgacgtgggg cttctcgcgc gacgatgtgg agagtgcgtt
     2221 cttcgccgac tacctcgagt cgggcgtcct gacgatctcg ccgttcgaga ccctggatgg
     2281 cttcggtgtc ggcaagctgg tcaagatggg cgtcgaagcc ggccgggcca cccggcccgg
     2341 gatccacgtc ggagtctgcg gcgaacacgg cggcgacccg gagtcgatcc acttcttcca
     2401 cgctgcgggt ctggactatg tctcgtgcag ccctttccgc gtgcccgtcg cgcgtctcga
     2461 agccggccgc gccgccgtcg atgcgagcga gcacgccgct tctgcctagc ggtttaagcc
     2521 agcggaccgg acgaccgcga ccaggtccgc cccggtatcg acgccggtan nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acgacctgcg ctgcctcgaa gcgctcgatc
     2701 gtcgggaccg gcaggacgct gcggttgcgg atggcggcaa cgattccgac gtcctgcacc
     2761 gggatcgagc cgtcgtcgac gcgggaatac aggtcggcct gcggccgagg gagcccgtat
     2821 gccgtcaggt ccggcatcga gatgcgttcg acgacagcgc cgaggcggtc cacgacccgc
     2881 ggcggcaggc gacggacggc gaccccggtc cactgctgcg gcaccggtcc cgtggtgcgg
     2941 cgcaggatgt gcggcgcggt gcggtaggac agccataccg gcgacaccgc atgctcggcg
     3001 agatcctgcg cgatctcgca gccagtgttt ccggtgccga cgacgagggc ggaccgcccg
     3061 atgaacggcc cggggttgcg ataggccgag gcatggatga cctcaccggt gaacccgtcc
     3121 tgtcccggcc acgacggggc gaccggcgtg tggttgtagc ccgtcgccac gacgacgtgg
     3181 tcggctagcc atcgcccacc gcccgcgtcc agcaccaccc acggtgcccc gtcgatatcc
     3241 gaaccatgct ccacggcaac gacgtccact ccggtgcgcg ggtcccgcac cgagaatgat
     3301 gatccgttct tgtgccgcca cgcgggccct cctgggtgtg ccgttactcg gcagtaaccc
     3361 tacagagggt cgacgccgca gctgacgttc gggtcgacgc cgacgtgatc gccctcaagg
     3421 cgttaattca gagaggggcc gacggatggt ggcgcgagcg gccgggcctg cagcgcggcg
     3481 gctaacccca cctcgcgccg cacacgggcc accagttcct gcaggcgttc gctcgtcgtg
     3541 acggcctcca gcacgtgctg gcgttccgca aggggcaatc ggagctggtc gaggactgcg
     3601 tatgccagtg ctctcgcccg gtccccgtcc accccaccgg caacgggagc cggcggatcg
     3661 agcagcgccg gagcgccgac gctgtcggcg taagtgagcc acgcctcgcg gagctggacc
     3721 accagctcgg gcagcgcgtc gacatcgccg ggctcctcat cgagccaggt cacctcgcca
     3781 acgagatagt cggccccagg cacccgcgca tccagccgga agcgttgcac cccaacgcaa
     3841 cccacgcgga tcccggccgg aatcgggtgg acgctcacga cccggacgga ggtgcccacg
     3901 tcatacagct gctcggctcg cccggctccc acctcgtgcc cgcgacggat cgcgaccact
     3961 ccgaagatcc gctcggtctc gggcaacgca agcacgtcgg cgagcagcgc ccgatagcga
     4021 ggttcgaaga tcaccagcgg cagctcccct cccggcacgg cgatcgtcgc cagcgggaag
     4081 agcggcagcg ccggcatacc gaaagggatc agacagcggt cgagggcgcg tgacgcttcg
     4141 tgcgcgcccc gccctggccc ttgagcacct tgccgagctc gccgcgcacg accgaccagc
     4201 gggtgcggcc gggctcctcg ttggggaggg cgaggcggca taccttgcga tagacctggt
     4261 aggcctgctt cagcaacgga ccactcgtgt agtgaagatc gttgcgctgc atcagatccc
     4321 ggatctgggg cgagatcgtc tcgtagcggt tggacggcat gtccgggaag aggtggtgtt
     4381 cgatctggtt gctcagattg ccgctgagaa tgtgcagcag cgtcggcccg gagatattgc
     4441 ccgagccgag catctggcgg atgtaccact cgccgcgggt ctcaccgtcg atggagtcgg
     4501 tctcgaaggt ctggatgtcg tccgggaagt gcccgcagat gatgaccgtg ttggaccaca
     4561 cgttgcgcac gatgttggcg gagaagttcg ccagcaaggt ccagccgaag ttggggcccg
     4621 acagcagcgg gtgcacgaca tagtccttgg tgcactgctt gcggatcttg gtgagcatct
     4681 cgtgcaggtt ggccttgaag gtggccttgt cctcgtcgga gagctggtcc cagttctgca
     4741 ggttgccgcg caactccagg tcgtaggccg cgatgccgta ttcgaagaac atcgcgttga
     4801 ccaggtgcca gatcggctgg ccgaggtgga acagctccca ctgctggtcc ttgtcgacgc
     4861 gcagcaggcc gtagccgagg tcggtgtcct tgccgagcac attggtgtag gtgtggtgca
     4921 gctggttgtg gctgtgctgc cactgccgcg ccggggaggc catgtcccag tcccacgtcg
     4981 aggagtggat cttggggtcg cgcatccagt cccactggcc gtggaggatg ttgtggccga
     5041 gttccatgtt ctcgaggatc ttcgccagcg acaggccggc ggtgccgagc cagaacgccg
     5101 gccgcacgcc cttgaccttc acgccgctga agagcaggag agcgcggctg ccgatctcga
     5161 gggtgcgctg ggtcttgatg atgcgccgga cgtatgccgc gtccttggcc ccgcgcgagt
     5221 tcatcacctc ggcgcggatg gcgtcgagtt cgatgccgat ctgctcgatc tgctcgttgg
     5281 tcaggtgccc catcgggttg ggctcgcccg acttcttggg cgggtgggtg cgctcggcgc
     5341 cgccgctcac cgacagccgg gtgttgagcg tatggtgcgg cgcggccaca tgcgtgggct
     5401 cgctgacgtg cggtgccgtc ggggagatcg acgtgcggtc cggtgccagg gtcatgttgg
     5461 ggtgtcctct cggggggcgt ccgctatcag acttccagat cgcaggaccc tgcgaccgcg
     5521 ttgatgcagg tctgaattgg caattcgtca ccaggctcga cggtggtgat ctcaccggtg
     5581 cgcagatcgc ggatcgcgcc ctgccgcatc gtcaccacgc aaccgaagca gatgcccatg
     5641 cggcatccgc tcgtcatgag gacgccggcg tcctccgccg cgtcgaggat cggggtggaa
     5701 ccgtcggcat cgatggtcag ctcgctgtcc agcagcgtga tctcgccgcc ctcgccgggc
     5761 tcgatgatcg tcggccggaa tcgctcgacg tgcaacgcgt cggtgagttc gtgggcggcc
     5821 cagtgcatct cggcggcatc gaggaggccg gtggggccgc atacccagac cttgcgctca
     5881 cgccagtcgg ggacctcggc gtcgaggccg gcgaagtcga gcatcccgtc gacgtcggta
     5941 tgccgcacgc tgagccggat ctgtccgctc gccgcgcgct cggcgagctc ggcggcgaag
     6001 accacgtctt ggggctgggg cgccgagtgc agcagcacga tgtcggcggc cgggtgcagc
     6061 ggcaggttgc gcagcatgcc catgacaggg gtgatgccgg agccgccggt gacgaagagg
     6121 atcttggcgg ggagcgtggc cgggagaacg aagtcgccga tggcctggtc gagcatgacc
     6181 agggtgccgg gccgggcgtc gcggacgaga tggttgctga ccaggccgtc caggatcgcc
     6241 ttgggggtga tggagatggt gccgtcggca gcgtcggacc gggaggtcag ggagtaggcg
     6301 cgccagtggc gcacaccgtc gatgtcgatc ccgatgcgga tgtattggcc cgggatgtgc
     6361 ccccgccagg acttgccggg ccggatgacg acggtcgcgg cgtcgtcggt ctcccggtgg
     6421 atcgcgacga tccgcccgcg caactcggca ccggcgcgca ggggcgcgat catgtcgaga
     6481 atctcggtgg gcacgatggg ggtggcggct gcagctgcta ccttccacac cccactggtg
     6541 agagcgggga gcactccacg cgccggacgc ggcggggcgg ctgtcatatg tcccattcta
     6601 acctacggaa ccgtaagtta ctcccgcgta ggttgtggtg cccgtcgttg ataacaggta
     6661 ggacgtcaat tcgcgggctg gcctgtgcac tgtttcgcca gcctcatacg tttcgcgtat
     6721 gaggctccgc ttccgtcgcc gtctccccct cgttgccacc gcgctcgccg tgtcggccgc
     6781 caccgccgtg accggcgggt cgcccgcgac cgcgtccgag tcctcccccg ccccggctcc
     6841 cggcctgcgc tcggccgcct tccctgacaa ggccctgaac ggccacctgg tgcgcccggc
     6901 cagcgaactc ggcgcggcgt cggcacccaa ggcaggcgcg cgacgcacca gcggggcacg
     6961 cgcagcagga ggcaagtatg cgggtgagca ccgcatctac tacctcccgg tgagttggac
     7021 cacgagcgag ccgtcgcagg atccgatcac caatgccgac acctacgtcg gccgcgccga
     7081 ttcgtattgg caggacgtga gtggcggcag cgtcgacgtg acgctcgggt gggccgatga
     7141 ctggtggcgt attcacctct cgtcggccga agtggccacc tgcgaccgga aagcgatctg
     7201 gcgggaggcc agcgccctga tcgagggggg cggtggtgac ttcgaccacg tcctggtcaa
     7261 tctgcccaag aatcctgcgt gcgactgggt cgacgtcgag tatttcggcc ccacctcggc
     7321 gggatacggc ctgacgatga ccaacggggt ctatgtcccc aaggacgacg tcatcaaccg
     7381 ggcgatgatg cacaacaact gggtcctgtc gaccgatcag accatgtgca aggacagctc
     7441 cggcaagccg gtaccgatct cgggcgactg ctcctaccgt ctgtacgtcg acccatggag
     7501 cccgttgagc gaccagccgt tcgggtacac gcggaccggg ttcccgcacg cccgcgagct
     7561 actcgacttc ggggtcttcg gcagcgacga atggacgacg gtcactccgg gcgatctccc
     7621 gcagaccgtc acgctccagc gcctgcaggc gcccggcaat ggcacgcagg tgctcggttt
     7681 caccctggac ggctcctggt acgacatcga ataccgggcc gcggtgggcc gtgacgactg
     7741 gatcgacaac aagacctaca ccgactcctc cggagtgacc cgcacgactc ccggtggcgg
     7801 ggtcacgctc gtgcgcacca gaatccctgt ggagcaaccg gatggcagca cgctggtctg
     7861 ggacgggtcc ctcgtcgact tccacccgcg cgggaccagt tcgagctatg tgacggagcg
     7921 ccatcccggc ctcgagccgg gcgagagtta tacggctccg gacggcctgt tcaagatctc
     7981 cgtcatctcc gccaacctca gttcggcccg cgtcgagatc tcgttcccgg ggctgcgcaa
     8041 ggttgcgcgg tggtccggga aggatcggta tgccgcctcg gtagagatgt cgcagcagtc
     8101 cttcgccccc ggcgtggacc gggtcctgat cgcctccggc gaggtctaca ccgacgctct
     8161 ctccggggca ccggtgggcg gccgtgacgg cgacccgatc ctgctgacca aggcggacga
     8221 gctgcccagc gtcgtcgaag ccgagctgcg gcgcctggat ccggcgtcgg tggtcatcct
     8281 cggcggtccg gccacgatca gcgaccgcgt ggcgagccgc ctggccagcg tgaccggggt
     8341 gaccccgcag cgatggtccg gcgccgaccg cttcgtcacc tcggcccaga tctccgagcg
     8401 cagctacgag gccggaggcg tggacacggc atacgtcgcg tcgggccgcg tcttccccga
     8461 cgccctctcc ggcgcgcccg cgtccggcaa ggcgggcggg ccggtgctgc tcgtggacac
     8521 cgacgagctg ccctccgaga tcgccgccga actgcggcgg ctctccccgc gccgcatcgt
     8581 cgtcctcggc ggcaccgcga cgatcgccac cggcgtggag acgcaactgc gcggcctggc
     8641 atcgtcggtc gaacgatggg ccgggccgga ccgctacgca accgctgtgg aggtgaccaa
     8701 gcactcctac gacccgggcg tgggcacggc atacatcgcc tcgggtctgg tcttcgccga
     8761 cgccctgtcc ggcgccccca tcgccggcat gaccaagggc ccgatcctgt tgacggacac
     8821 tgcaaaactg ccgtcggttg tggcagcgga gttggagcgg ttgaagccca agcgcatcgt
     8881 cgtcctcggc ggcgcgaaca cgatcaccta tgcccagcag gctcaactcg gccggtacgt
     8941 cgtttcgtag cgtccgcccg tcctgaggtt gtcaggcccg gccctcccgc gatgagagga
     9001 tgccgggcgt gaccactgcc gggctcgtct tcgccgccgc cgcggccctc atccacgtct
     9061 atatcttcgt gctcgagtcg ctgcgattcg atcagccggc gacgtggaag gtgttcgggg
     9121 tcaaggggcc ggaggctgcc gaggtgatcc ggccatgggc cttcaaccag ggcttctaca
     9181 acctcttcct cggcctgggt gcggccctcg gggtgttcct gtgcgcgttc aaccggcagg
     9241 gcacgaccgc ctacgtccgc ggcgccgcca tcgcgctctt ctgctgcgcc gtcatggtcg
     9301 cggcggcgct cgtcctgctg gtcaccgacc gccgcaaggc ccgggccgcc ctcgtccagg
     9361 gccttgcccc cgcgctcgcc gtggcgttcc tgctggtggg ctagccccgg gccggacaag
     9421 gtcgccgcgg accctaacca tcgcgctacc cattggcatg tggggtgatc acaacaagtc
     9481 ccaatggcta gtgacgtcgg tctggtccgt tcgaaagccc ggcgccagag tgggcgcctc
     9541 agcccggcag gtgggcgtcc agccaggcga tgaggtcggc ggtgacctcg tcgcggttgg
     9601 tctcgttgag cagctcgtgc cgggcaccga tgtagtagtg cgtggtcacc tgctgcaccc
     9661 cggccgcgcg gtactggttc gccaccgcct ccacgccttt cgtcttcgca ccgaccggat
     9721 ccatcgagcc ggcaatgagg tggaccggca aggtcttggg caccttggcc gtcgtgtcgc
     9781 ggcgatggac gtagttcagg ccgccgagca ggtcgatcca gaaaccggtg gaaaagacgt
     9841 tgccgcacaa cggatccgcg acatacttgt cgacctcgtc ggggtcgcgg gagagccagt
     9901 cgaagtcggt gcggttgggt ttgaagtcct tgttgtactg cccgaaggac atcgcgttga
     9961 gcagccccga cgcgtgggtg cgccccttca gccgggcctg cgcctgcgcc acgacgatcc
    10021 ccaccttccc cagcgccccg gcatcccccg cggtgccgga caggatcagg ccggcgaggt
    10081 cggcgccgta ggccgcggca tacgcccggg cgaggaagga gcccatgctg tgacccagca
    10141 ggacgaccgg cacccccggg tatgccgccc gcgccacctc cgtgaccgcg cgcaagtcct
    10201 ccacgacctt gccgaaacca tcggtgtcgg cgaaatagcc gtgcccctcg ccgacggggt
    10261 tggtatgccc gtgtccccgg tgatcgtttg cgtacaccgc ataccccgcg gcggtgagcg
    10321 cgtcggcgaa gcggccgtac cgctgcgcgt gctcggccag gccgtgggcg atctgcacga
    10381 tcgccttcgg ctgtccttgc ggggtccaga cataggtatg cagtgcggtg ccgtcggcgg
    10441 cggtgaccgt cgtggtcgtc atgcgtacgc ctttcgcaaa tctgtcgtcg agtcgtcaat
    10501 gtagtccggt cggtgggtga cagatccggt ggatcacgtg agactgggca ccatgaacgc
    10561 aggggacaac acaccgacat acggacacag cggcgacgaa gcacaagacg ggcagatccg
    10621 gcccatcccc gtgccgagcc agggcggggc aagcgtgccg gccgagcgca agggcggcac
    10681 gatggccggc ctcatggcca tcagcagcac cttggcgctg atcgccttca tgatcatcgg
    10741 cttcgtcttc gacgggtggg cgtggggctg ggtcgtcttc ctgatgccgg ggctgctcaa
    10801 aagctggcag gcagcgcaga actaggcacg cgcaagctca cacgcggcac ctctgtcccg
    10861 tgagggcacg gtttcactag ggttctgggc gtgagccaaa tgaacctgcg ggagtacatc
    10921 gacagcctca agaccggggg ctacaccgtg cgcgacgatc acggtgacga tccgatgctg
    10981 atctccccgg agggcaaggc cgtcgagacc tggcgggaga actacccata cgacgaactc
    11041 atgtcgcgca atgactacga ggtcgagaaa tacctgctgc agatcgagtt gctgaagttc
    11101 caatacttcg tgcaggacat gggcgaaaag cacgtcatcg tcttcgaggg ccgcgacgcc
    11161 gccggcaagg gcggcacgat caaacggttc atggagcacc tcaatccgcg gtcggcccgc
    11221 gtcgttgcgc tcaccaaacc gacgaccacc gagcaggggc agtggtattt ccagcgctac
    11281 gtcgcgcacc tgcccacggc cggggagatc gtgctcttcg accgctcctg gtacaaccgg
    11341 gccggcgtcg agcgggccat gggcttttgc accgaggacc agtaccaggt gttcatgaga
    11401 caggctcctc gcttcgagga aatgctgatc gacgcgggtg ccacggtcac gaaattgtgg
    11461 ttctcggtca cccagcagga gcagcgcacc cggttcgcta ttcggcagat cgacccggtg
    11521 cggcggtgga agctatcgcc gatggacctg gagagcctgg acaagtggga ggcctacacc
    11581 gaggccaagg aagcgatgtt caagttcacg gacaccaagc attcgcagtg gcacacgatc
    11641 aagtccaatg acaagaaacg cgcccgcatc aatgccatgc ggctcttctt gaatatgcac
    11701 gactacgacg gcaaggaccc ggaggtcgtc ttcgagcccg acccgctcat cgtcggccgc
    11761 ggcaagaaga ccatcggcga ctgactgccc ggcacgggga cggctcagcg gccgtcccgc
    11821 aatccgggat
//
LOCUS       DAOPJG010000100        11757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5731_length_11757_cov_7.471800, whole genome shotgun sequence.
ACCESSION   DAOPJG010000100 DAOPJG010000000
VERSION     DAOPJG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5731_length_11757_cov_7.471800"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..629
                     /gene="purN"
                     /locus_tag="PKU75_10045"
     CDS             39..629
                     /gene="purN"
                     /locus_tag="PKU75_10045"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPF81262.1"
                     /translation="MPTVIPARLVVLVSGSGTLLQALIDAAADAAYGAQIVAVGADRD
                     DTLGEQRAARAGIPTFVEKVSDHPSREEWDAALTARVAAYDPDLVVSAGFMKVLGPAF
                     LAAHRTINSHPALLPSFPGAHGVRDALAHGVKLTGCTCHWVDSGVDTGPIIDQRAVRI
                     EDGDTELSLHERIKVLERDMLVDVVRTLASARSRPN"
     gene            640..2265
                     /gene="purH"
                     /locus_tag="PKU75_10050"
     CDS             640..2265
                     /gene="purH"
                     /locus_tag="PKU75_10050"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPF81263.1"
                     /translation="MSSVSAPGPTQDIRPVRRALVSVYDKSGLEELVRGLHEAGVALV
                     STGGSAKFIAGLGLPVTEVADLTGFPECLEGRVKTLHPVIHAGVLADTRKPDHLEQLA
                     GLEIKPFELVVCNLYPFAATVASGAGPDECVEQIDIGGPTMVRAAAKNHPSVAIVTDP
                     AAYPMVLEAVTSGGFTFEQRKLLAAKAFQHTADYDNAVANWMGNAYVDTSGGSGFPAW
                     TGATWTKAQSLRYGENPHQRAALYRSGRAGLASAEQLHGKEMSYNNYVDTDAAVRAAY
                     DQGDQPTVAIIKHANPCGIGVGDTVDAAYAAAHACDPVSAYGGVVATNRTVNRAMALA
                     LNDTFSEVVAAPGFDADALDVLREKKNRRLVQLPDGMQAARDAVEYRPISGGVLMQTV
                     DRLDAVVEDASGGVSGGDEAANWRLVAGSAADAATLADLQFAWRAVRSVKSNAILLAR
                     DGASVGIGMGQVNRVDSCRLAVSRAGEERARGAVAASDAFFPFADGLQVLLDAGVEAV
                     VAPGGSVRDEEVVAAAEAAGVTLYFTGTRHFAH"
     gene            complement(2354..3028)
                     /locus_tag="PKU75_10055"
     CDS             complement(2354..3028)
                     /locus_tag="PKU75_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81264.1"
                     /translation="MMTNLEHPLVQDYLARLRAQAAALPEGEARELVADIEEHFVAAE
                     REGGTSEADIRTLLDRLGTPESVVAAAAPPAPPAPAGSGIPRREGMTVMLLIVAEVLV
                     LTVLLAPIGIIVWLVGLVMLVLCRRWNTAQRAQAWVVLASGVPVALLALAAIGMTAYT
                     DMSCAPDGSNAATCDPTASAPPAWVPWLVGLLGIGFGLLQVVIARRLLAAVGEHGPAP
                     KGLRMR"
     gene            complement(3025..3372)
                     /locus_tag="PKU75_10060"
     CDS             complement(3025..3372)
                     /locus_tag="PKU75_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006213685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF81265.1"
                     /translation="MAAEGEGKALTQLRRGALEHCVLALLDDGERYGYDLVGELSEAG
                     LVAGEGTVYPLLSRLRKDALVTTTWRESESGPPRRYYSLTPTGRQALGDFRGAWGEFK
                     SAVDRLLDGKARS"
     gene            complement(3513..4220)
                     /locus_tag="PKU75_10065"
     CDS             complement(3513..4220)
                     /locus_tag="PKU75_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81266.1"
                     /translation="MTTLTIPAHFNGPPRSANGGWFAGSAAGFVRDSVPDADAVRVRL
                     SAPPPLEVPLTVSGDAAAYRMTHGETLVGTAEPAAALAPTPVGPVSFADAVAAGERYE
                     GLAEHPFPTCYSCGTGRADGLGLRPGSLGRADGAYAAAWVPTEVSVPIVWAALDCPGG
                     WSAGIAGRPMVLGTMTAAVSGLPEVGEECVVMAWPGSSSGRRFDSGTALFGADGRLLG
                     RADAVWIAVDPAAVRPR"
     gene            complement(4242..5330)
                     /locus_tag="PKU75_10070"
     CDS             complement(4242..5330)
                     /locus_tag="PKU75_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HPF81267.1"
                     /translation="MLTRARWYHVLPVFAALALALTLGRELGAIGVGVVALLLGGAVL
                     AAVQHAEIVAHRVGEPFGSLVLAVAVTVIEVSLILTLMLTAPEKSATLARDTVFAAVM
                     ITLAGIIGAALFFATRGSRIPRFNAEGSGGALATVATLTTLTLVLPSMTTGAPGPRFT
                     TGQLMFAAVSALVLYGAFVATQTVRHRDFFLPVAKDGSVIATDDDHADPPSNATAWTS
                     LAFLLLSLIAVVGLAKVESPAIEDAVNAAGLPHSFVGVVIALLVLLPETLAAVNAARR
                     GRLQTSMNLALGSAMASIGLTIPALAIATRFVDAPLELGLGPTHITLFVLAVVVSALT
                     IVPGRATRLQAVVHLGIFAAFVFLAMSP"
     gene            complement(5406..6593)
                     /locus_tag="PKU75_10075"
     CDS             complement(5406..6593)
                     /locus_tag="PKU75_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81268.1"
                     /translation="MLTGSALITALAGCGPANAPTQPVRVVQANAKPTTSKAPRPIGD
                     GSTAHTGPQPHQPTLPDLAAGSAPPQFVVFSWDGGGETDAHLLTRFRTVAKEIGASMT
                     IFLSGIYLLREADAKRYHAPRHDPGDTDIPFLSDGSVKRTIEGVGAAWLEGHEIGTHF
                     NGHFCGPGGVGDWTSKEWRDEIDQAVWMVKHWRTTTGWTSIPSLPFDYDKELIGSRTP
                     CLLGSDALLPTAQKLGWRYDSSTSTRQVWPTRFEGTTMWNLGLSQIPFPGHKFEVLAM
                     DYNYMANQSGPQPGKDASKYPGWKTEALGALLAGHQRAHTGNRAPLVIGNHFEQWNGG
                     IYMDAIEEAMRTLAKAPDTYLVSFRQLVDWLDRQDPILLGQLTHLDVGQVPEGGWAGF
                     GAE"
     gene            complement(6720..8192)
                     /locus_tag="PKU75_10080"
     CDS             complement(6720..8192)
                     /locus_tag="PKU75_10080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPF81269.1"
                     /translation="MPLPSISPSAAAIDAAGLLALVDALEAHPGIEMHSFAIARAGRV
                     VAEGYWAPYAAERPHLLYSVSKSWTATAVGVLVDEGRLALSDPVCRDLPGVDFAALPA
                     RWQQVTVGDCLRMATGHDQEAWLWGSDESGVATRPLLDSGLDPMVARILAHEPEHEPG
                     THFAYNQVATYLVAAAARAVAGTSLVGILRERVLEPLGVPELLWKTTGSGAELGFSGS
                     FARTRDVLALAQLYLDEGRVGDRQVLSPEWVECARTPSGLVNPDPGGPDWAQGYGCSF
                     WSSRHGYRGDGAFGQFAIVLPQHRIAVATTAETGDMQAILDAIWTHLLPAVDRAVDRP
                     GDPAADTAADTALAERLAGLRIAPPPPRPFAGGQWSRTSGDVAQSYSAARVRPVEPGG
                     GWELTLTRDGTDLTVAVGAGAWAESEWRADGIRLPLVAAGGGTGDGGFAAQIRLVETP
                     HTVHLRATPAPPGGPGGFDLSWSLPPLHGPDPLRQSARYA"
     gene            complement(8203..8625)
                     /locus_tag="PKU75_10085"
     CDS             complement(8203..8625)
                     /locus_tag="PKU75_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81270.1"
                     /translation="MLSVATATALAAAGILWEPQPGDRFAISSPELDGDQFWISELTI
                     EVHHYQDETILGFNGTTEWALDSVPLDKAIWLPREDQLRALIGDRLESLRRTPDGWAV
                     TYRTEAPGGQPATATHADPEEAYAAALLHIAGAFTPSE"
     gene            9012..10667
                     /locus_tag="PKU75_10090"
     CDS             9012..10667
                     /locus_tag="PKU75_10090"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPF81271.1"
                     /translation="MHSSFSEGAGALKAFGTPGQESASMESHAYSLSTMGFDLCMYTD
                     HDHRMAGANWGATYVPYPTREDFANPGWTYSSQRYGSVAGGHNFGAGGLTVFNRNGLS
                     GGVTLAYCDSGNKRWTYRTNLGGMTLALAAIAPETGYAEFRLQSSHRPATAGRPEGSY
                     VLRYLLRTDVTERVTEVAGLTVIVSVPAQSGTTTVVPIDPCADLAAAYPDLGIRVHDM
                     SAYGFWFGAGGGPECDNAALFQCLDIARTADVGAALALQRELLGRLQAGYPDIRLVQG
                     LELSYATHVNWLSNASTDITSTPPTGGSMSTYLRNSIAMIRAAGGASSYNHLFGATRG
                     PLITGSARTGKIKTTATQLLANRLYGADILEVGYTLRGGMDLAAHLEVWDILLAAGMR
                     VIANGATDNHNGSLSSYARDSNNFATDVLASTSDPALVIPALQQGRAFVSQHGKFGGL
                     LDLSATGAVMGSTVTSPKSTVNVTVTHEGCSGMSVRVLQYAVHGSTTVNARRAPLKNV
                     SINPGGRSTTALSFQNVNSYIRSEVWSGGKRVAFSNPLFIRRR"
     gene            10743..>11757
                     /locus_tag="PKU75_10095"
     CDS             10743..>11757
                     /locus_tag="PKU75_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPF81272.1"
                     /translation="MTKRSLRNTVATIGCATLALAGLTAAPADAAPGDVQINELLYHA
                     ADADLTYGALEFVELRNTGSEPVDVGGWQFTAGITGTAIPAGVTIPAGGYLVGTNDAA
                     LFQARFGFAAGFVFATGEALSNGGELVTLADAAGGVIDSVLYDDAVPWPLSPDGAGPS
                     LELDPSATDNAATSWHASSVDFGTPAAANSAAPMALTGVGPDSVTPEPGADVTLTATA
                     PAGSTVTLTYKVMYAADVVIPMADDAASPGGAADGVYAATIPGAGAGELVRYRVSATK
                     GTQAASYPAEGDSRPYDGYVVRDPELDSATYPVLQWFMSDEVYQDMITNHRCDDYDAQ
                     ATFA"
BASE COUNT         1826 a         4175 c         4041 g         1715 t
ORIGIN      
        1 gcgccggctg gtcggcgccg gccgacccat cgctaggctt gccaaccgtg atccctgccc
       61 gcctcgtcgt cctcgtctcc ggctccggca cgctgttgca ggcgctgatc gacgcggccg
      121 ccgacgcggc atacggcgcc cagatcgtcg ccgtcggcgc cgaccgcgac gacaccctgg
      181 gggagcagcg ggcggcgcgg gccggcatac ccaccttcgt cgagaaggtg agcgaccacc
      241 cgagccggga ggagtgggac gccgccctga cggcgcgggt ggcggcatac gacccggacc
      301 tggtcgtgag cgccggcttc atgaaggtgc tcggcccggc cttcctcgcc gcccaccgga
      361 cgatcaactc ccatccggcc ctacttccga gtttccctgg ggcacatggg gttcgcgacg
      421 cgctcgcgca cggggtcaag ctgaccgggt gcacgtgcca ttgggtggac agcggcgtcg
      481 acaccgggcc gatcatcgac cagcgggcgg tccggatcga ggacggcgac accgagctga
      541 gcctgcacga gcggatcaag gtcctcgagc gcgacatgct cgtcgacgtg gtgcggacac
      601 tggcctcagc ccggtcacga ccgaactaga ctcgagcaag tgagtagcgt cagcgccccc
      661 ggtcccacgc aagacatccg cccggtgcgc cgggccctcg tctcggtcta cgacaagagc
      721 gggctggagg agttggtccg tggcctccac gaggcgggcg tcgcgctcgt ctcgaccggc
      781 ggctcggcga agttcatcgc cggcctcggc ctccccgtca ccgaggtggc cgatctgacc
      841 ggcttccccg aatgcctcga ggggcgcgtc aagaccctcc accccgtcat ccacgccggc
      901 gtgctcgccg acacccgcaa gcccgaccac ctcgagcagc tggccggcct cgagatcaag
      961 ccgttcgagc tggtcgtctg caacctctac ccgttcgccg cgaccgtcgc ctcgggggcc
     1021 gggcccgacg agtgcgtcga gcagatcgac atcggcgggc cgacgatggt gcgcgcggcc
     1081 gccaagaatc acccctccgt cgccatcgtc accgaccccg cggcataccc gatggtgctg
     1141 gaggccgtga cctccggtgg gttcaccttc gagcagcgga aactgttggc cgccaaggcc
     1201 ttccagcaca cggcggacta cgacaacgcg gtcgccaact ggatgggcaa cgcctacgtc
     1261 gacacgtccg gcggcagcgg tttcccggcg tggacgggtg cgacgtggac caaggcgcag
     1321 tcgctgcgct acggcgagaa cccccaccag cgcgccgcgc tctatcgctc cggacgggcg
     1381 gggctggcca gtgccgagca gttgcacggc aaggagatgt cctacaacaa ctacgtcgac
     1441 accgacgccg ccgtgcgggc cgcctatgac cagggcgacc agccgacggt cgcgatcatc
     1501 aagcacgcca acccctgcgg gatcggcgtc ggcgacaccg tggatgcggc gtatgccgcc
     1561 gctcacgcct gtgaccccgt gtccgcgtat ggcggcgtgg tcgccaccaa ccgcacggtc
     1621 aaccgcgcga tggcgctggc gctcaacgac accttcagcg aagttgtcgc ggcgcccggc
     1681 ttcgacgctg acgcgctgga cgtgttgcgg gagaagaaga accgacgcct cgtgcagttg
     1741 cccgacggta tgcaggcggc tcgcgacgcg gtggagtacc gcccgatctc gggtggcgtg
     1801 ctcatgcaga ccgtggaccg gttggatgcg gtcgtcgagg atgcctcggg cggcgtctcc
     1861 ggtggggacg aagcggcaaa ctggcgcctc gtggcgggat ctgctgccga tgcggcgacg
     1921 ctggcggatc tccagttcgc ctggcgcgca gtgcgatcgg tgaagtccaa cgccatcctc
     1981 cttgcccgcg atggcgcgtc ggtcggcatc ggcatgggcc aggtcaaccg ggtcgactcg
     2041 tgccgcctgg ccgtctcgcg ggcgggggag gagcgggcgc gtggggcggt cgccgcctcc
     2101 gacgccttct tccccttcgc tgacgggctg caggtgctgc tcgacgccgg cgtcgaagcc
     2161 gttgtggcgc cgggcggttc ggtccgcgac gaagaggtcg tcgccgcggc cgaggcggcc
     2221 ggcgtgaccc tgtacttcac cggcacccgc cacttcgccc actgacactc cgaagctgtc
     2281 gctgtcgcgc gagtggctgt cacgtgagtg gcagcgacga aatgacgtcg ctgccactca
     2341 cctgacgagt tcgtcagcgc atccgcaggc ccttgggcgc gggcccgtgt tcgccgacgg
     2401 ccgccagcag ccggcgggcg atgacgacct gcagcagccc gaacccgatc cccagcaggc
     2461 ccaccagcca cggcacccag gcgggcgggg ccgacgcggt cgggtcgcag gtcgcggcgt
     2521 tgctgccgtc gggtgcgcac gacatgtcgg tgtacgcagt cataccgatc gccgccagtg
     2581 caagcagcgc gaccggcacg ccgctcgcga gcacgaccca ggcctgggcg cgctgggcgg
     2641 tgttccagcg gcggcacagg acgagcatga ccaacccgac cagccagacg atgatgccga
     2701 tcggggcgag gagcaccgtg aggacgagca cctcggcgac gatcagcagc atcaccgtca
     2761 taccctcgcg gcgcgggatg cccgacccgg ccggcgcggg tggcgccggg ggagcggcgg
     2821 cggcgacgac cgactcgggc gtgccgagcc ggtcgagcag ggtgcggatg tccgcctcgc
     2881 tcgtgccgcc ctcacgctcc gctgcgacga agtgctcctc gatgtccgcc acgagttcgc
     2941 gcgcctcgcc ctccggcaag gcagccgcct gtgcgcgcag ccgggcgagg tagtcctgca
     3001 cgagcgggtg ctcgagattg gtcatcatga ccgtgccttt ccatcgagaa gtcgatcgac
     3061 agcggatttg aattcgcccc acgcgccccg gaagtcgccg agagcctggc gcccggtcgg
     3121 ggtgagggag tagtagcggc gcggcggccc ggactcggac tcgcgccatg tggtggtgac
     3181 cagcgcatcc ttgcgcaacc ggctcagcag cggatagacc gtgccctcgc cggcgacgag
     3241 gcccgcctcg gacagctcgc cgacgaggtc gtacccgtag cgctcgccgt cgtcgagcag
     3301 ggcaaggacg cagtgctcga gggcgccgcg tcgcaactgc gtcaatgcct tcccctcgcc
     3361 ctctgctgcc atgcctcgca aggtagcacg cgagtacctt gcaccgcaag caacctggcg
     3421 tcactcagca gaggttggtc aaatccgctc acctagggag gtctgctgat ttgactaacc
     3481 tctgcgagca gaggttgctc ggtctccccg cgtcagcggg ggcgcacggc ggcggggtcg
     3541 acggcgatcc agacggcgtc ggcgcggccg aggaggcggc cgtcggcgcc gaaaagcgct
     3601 gtgccggaat cgaaccgccg tcccgagctg ctgcccggcc aggccatgac cacgcactcc
     3661 tcgccgacct ccggcaggcc ggacacggcg gcggtcatgg tcccgagcac catcgggcgc
     3721 cccgcgatgc cggccgacca gccacccggg cagtccagcg cggcccacac gatcggcacg
     3781 ctcacctcgg tcgggaccca cgcggcggca tacgcaccgt ccgcgcgccc caggctcccc
     3841 ggacgcaggc ccagcccatc ggcgcgcccg gtgccgcacg agtagcacgt cgggaacggg
     3901 tgctcggcca ggccctcata ccgctcaccc gcggccaccg cgtcggcgaa actcaccggc
     3961 cccaccggcg tgggcgcgag cgcggcggcc ggctccgccg tgcccaccag ggtctccccg
     4021 tgggtcatcc ggtatgccgc ggcgtcaccc gaaacggtca gcggcacctc gagcggtggc
     4081 ggagcggaca ggcgcacccg caccgcgtcc gcatccggca ccgaatcgcg gacgaacccc
     4141 gcggccgacc ctgcgaacca gccgccgtta gccgaccggg gcgggccgtt gaagtgcgcg
     4201 gggatcgtca gggtggtcat cgccccatcc tgccagcgcc gtcagggact catcgcgagg
     4261 aagacgaagg ccgcgaagat gcccaggtgc accaccgcct gcagccgggt cgcgcgaccg
     4321 ggcacgatcg tcagggccga gaccaccacg gcgagcacga acaaggtgat gtgcgtcggc
     4381 ccgagcccga gctccaacgg cgcgtcgacg aagcgggtcg cgatcgccag cgccgggatg
     4441 gtcaggccga tgctcgccat ggccgagccg agcgcgagat tcatggacgt ctgcagccgg
     4501 ccgcgccgcg cggcgttgac cgctgcgagc gtctccggca gcagcaccag caaggcgatg
     4561 acgacaccga cgaaggagtg cggcagcccg gccgcattga ccgcgtcctc gatggccggc
     4621 gactcgacct tggccagccc gaccacggcg atcagcgaca gcagcaagaa cgccagcgag
     4681 gtccacgccg tcgcattgct gggcgggtcg gcgtgatcgt cgtcggtcgc gatcaccgag
     4741 ccgtccttgg cgacgggtag gaaaaagtcg cggtgtcgca ccgtctgggt cgcgacgaat
     4801 gcgccgtaga gcacgagcgc ggagacggcg gcgaacatca actggccggt cgtgaagcgt
     4861 ggtccgggcg cgcccgtcgt catcgacggc agcaccaggg tgagtgtcgt caaggtcgcg
     4921 acggtcgcga gcgcaccccc ggacccctcg gcgttgaaac gcggtatgcg gctgccgcga
     4981 gtggcgaaaa acagtgcggc accgatgatt ccggcgagcg tgatcatcac tgcggcgaag
     5041 acggtgtcgc gggccagggt cgccgacttc tcgggcgctg tcagcatcag ggtcaggatc
     5101 agcgacacct cgatgacggt gaccgccacc gccagcacaa gcgagccgaa gggctcaccg
     5161 actcgatgcg cgacgatctc ggcgtgctgg acggccgcga gcaccgcgcc gccgagcagc
     5221 agcgccacca ccccgactcc gatcgccccg agttcgcgcc ccagcgtcaa cgccagcgcg
     5281 agggcggcga aaacaggcag gacgtggtac cagcgggctc gtgtcagcat gaatgtcttc
     5341 ccgcggcgtc ccgccgctgg acgtcgagtg ggatgagtgc accagtctat cgaaggacgg
     5401 tcgcgtcact ccgctccgaa gccggcccaa ccgccttcgg gcacctgccc gacatcgaga
     5461 tgtgtcaact gccccaacag gatcgggtcc tgccggtcga gccagtcgac gagctgccgg
     5521 aagctgacca gataggtgtc cggggctttg gcgagcgtgc gcatggcctc ttcgatggcg
     5581 tccatgtaga tgccgccgtt ccactgttcg aagtggttgc cgatgaccag cggcgcccgg
     5641 ttgccggtgt gggcgcgctg gtgcccggcg agcagcgccc cgagcgcctc cgtcttccag
     5701 cccggatact tcgaggcgtc cttgcccggc tgcggcccgc tctggttggc catgtagttg
     5761 tagtccatgg cgagcacctc gaacttgtgt cccgggaacg ggatctgcga cagcccgagg
     5821 ttccacatcg tcgtgccctc gaaccgggtg ggccacacct gccgcgtcga ggtcgagctg
     5881 tcatagcgcc agccgagctt ctgcgccgtc ggcaacagcg catcgctgcc gagcaggcac
     5941 ggcgtgcgcg agccgatcag ttccttgtcg tagtcgaacg gcagcgacgg tatggacgtc
     6001 cagccggttg tcgtgcgcca gtgcttgacc atccacacgg cttggtcgat ctcgtcgcgc
     6061 cactccttgc tcgtccagtc accgaccccg ccgggcccgc agaagtggcc gttgaagtgg
     6121 gtcccgatct cgtgcccctc gagccacgcc gcgcccacgc cttcgatggt gcgcttgacg
     6181 gagccgtcgg acaggaaggg gatgtcggtg tcgcccgggt catggcgcgg ggcgtgatag
     6241 cgcttcgcgt ccgcctcgcg cagcagatag atgccgctga ggaagatcgt catggaggcg
     6301 ccgatttcct ttgcgacagt gcggaaccgg gtcagcaggt gcgcatcggt ctcgccgccg
     6361 ccgtcccacg agaagacgac gaactgcggg ggcgcgctgc ccgcggcgag gtcgggcagc
     6421 gtcggctggt gcggttgggg tccggtatgg gcagtgctgc cgtccccgat cggtcgcggc
     6481 gctttgctcg tggtcggctt cgcgttggcc tgcacgacgc gcaccggctg cgtcggggcg
     6541 ttggccggcc cgcacccggc cagggcagta atcagcgccg acccggtcag catgatggcc
     6601 gatcgtcggt tggtctccac gggctccacc ccctctgcta ccactgtccc ggttcgcgtc
     6661 gcttcatctg tgacgacgcg ccgagcgggc ccgaggttgg gtgcgaattt taatgcgggt
     6721 caggcgtacc gggcgctctg ccggagcggg tccgggccgt gcagcggcgg cagactccac
     6781 gacagatcga acccgcccgg gcctccgggg ggtgccggcg tcgcccgcag atgcaccgta
     6841 tgcggtgtct cgacgagccg gatctgcgcc gcgaatccgc cgtcaccggt gccgccgccc
     6901 gcggcgacca ggggcagccg tatgccgtcc gctcgccact cgctttccgc ccacgcgccc
     6961 gcgcccaccg cgacggtcag gtcggtcccg tcgcgcgtca gggtcaactc ccagccgccg
     7021 cccggttcga ccgggcgcac ccgcgcagcc gagtatgact gtgccacatc gccactcgtg
     7081 cgactccact gtccgccggc aaacgggcgt ggcggcggcg gcgcgatccg tagacccgcc
     7141 aggcgctcgg ccagggcggt gtccgcggcc gtgtctgcgg cggggtcacc ggggcggtcg
     7201 acggcgcggt cgacggccgg gagcaggtgc gtccagatcg cgtcgaggat cgcctgcatg
     7261 tccccagtct ccgcggtcgt cgcgacggcg atccgatgct gcggcaacac gatcgcgaac
     7321 tggccgaagg cgccgtcgcc gcggtagccg tggcgtgatg accagaacga gcagccatag
     7381 ccttgggccc agtccgggcc gcctgggtcg gggttgacga ggccggatgg ggtgcgcgcg
     7441 cactcgaccc attcgggtga caacacctga cggtcgccga cgcggccctc gtcgagatag
     7501 agctgggcga gggcgagcac gtcacgcgtc cgggcgaagc tgcccgagaa gcccagttcg
     7561 gcgccggatc ctgttgtctt ccagagcagt tcggggacac cgagcggttc gaggactcgt
     7621 tcccggagaa tgcccacgag gcttgtgccg gcaacggcac gggctgcggc cgccaccaga
     7681 taggtcgcga cctggttgta ggcgaagtgg gtgcccggct cgtgttccgg ttcgtgagca
     7741 aggatccgcg caaccatggg atccaacccg gagtccaaga ggggccgggt ggctacccca
     7801 ctctcgtcgg aaccccacag ccaggcttcc tggtcatggc ccgtcgccat ccgcaagcaa
     7861 tcgcccacgg tgacctgctg ccaccgcgcc ggcagggcgg cgaagtcgac gccgggaagg
     7921 tcgcggcata ccggatccga cagcgccagg cgaccctcgt cgacgagcac acccaccgct
     7981 gtcgccgtcc acgacttgct caccgagtag agcaggtggg ggcgctccgc ggcatacggc
     8041 gcccagtatc cttccgcgac aacgcgaccg gcacgggcga tcgcgaacga gtgcatctcg
     8101 atgcccggat gtgcctcgag agcgtcgacg agggcgagca agccggcggc gtcgatagcg
     8161 gcggccgagg gcgagatgga ggggaggggc acgcgaccga ccctactcag atggggtgaa
     8221 ggctccggcg atgtggagga gggcggcggc atacgcctcc tcggggtcgg cgtgggtggc
     8281 ggtcgcgggc tggccgccgg gcgcctcggt gcgataggtg accgcccagc cgtccggcgt
     8341 gcgccgcaga ctttccaggc gatcgccgat cagggcgcgc agctggtcct cccgcggcag
     8401 ccagatcgcc ttgtcgagcg gcacggagtc gagcgcccat tcggtggtgc cgttgaatcc
     8461 gaggatggtc tcgtcctgat agtggtgcac ctcgatggtc agctcgctga tccagaactg
     8521 atcgccgtcg agttcggggg aggagatcgc gaaacgatcg ccgggctggg gctcccagag
     8581 gatcccggcg gcggccaggg cggtggcggt ggcgacgctg agcatgcgcc ctattgtgcg
     8641 cggcgcagtc cactttttgg gggacttgtt caggcattat tcgcttttta tccttgttcc
     8701 ggccgtgaga atgtcgccga tttgcggcgc cggatcgcgg gcagtggttt cggagaattt
     8761 cggggacgtg aatggatatt cggtcgcatc atgagctgac ggggatgctc gaagagccgt
     8821 ggcccgatcc ggagcacggc tgttgcggcg gcgcggagcc ggagctgccg agtgggagcc
     8881 cgagccggcg cgaactgctc gcatatgcgg ggctatcgcc tttgcttgca acaggtttca
     8941 cgcgattcga gactcccggc gagatcccgc gcctcgagcg gccggacagt gtcgttcgcg
     9001 ccgccctaca cgtgcacagt tcgttctccg agggcgcagg cgccctgaag gccttcggca
     9061 cacccggcca ggaaagcgcc tcgatggagt cgcacgccta ctcgctgagc accatgggct
     9121 tcgacctgtg catgtacacc gaccacgacc accgcatggc cggtgccaac tggggcgcca
     9181 cctacgtgcc ctatccgacg cgagaggact tcgccaaccc cgggtggacc tattcgagcc
     9241 agcgctacgg ctcggtggcc ggcggtcaca atttcggggc gggcgggctg acggtgttca
     9301 accgcaatgg cctcagcggc ggcgtcaccc tggcctattg cgattcgggc aacaagaggt
     9361 ggacctatcg gaccaacctg ggcgggatga cgctcgccct ggcggccatc gcccccgaga
     9421 ccggctacgc ggaattccgc ctccagagca gtcaccgtcc cgccacggca ggccgcccgg
     9481 agggcagtta cgtcctgcgc tatctgctgc gcaccgatgt gaccgagcgc gtaaccgagg
     9541 tcgccggcct gaccgtcatc gtgtctgtcc cggctcaatc cggcacgacc accgtcgtcc
     9601 cgatcgaccc gtgcgccgat ctcgcggccg cctatcccga cctaggcatc cgcgtgcacg
     9661 acatgtcggc ctacgggttc tggttcggcg ccggaggcgg acccgagtgc gacaacgccg
     9721 cgctcttcca atgcctggac atcgcgcgca cggcagacgt cggcgccgca ctcgccctcc
     9781 aacgcgaact gctcggacgc ctgcaggcgg ggtacccgga catccgactc gtccaaggcc
     9841 tggagctgtc ctacgcgacg cacgtcaact ggctgtccaa cgcctccacc gacatcacga
     9901 gcacgccacc caccggcggc tcgatgtcga cctacttgcg aaacagcatc gcgatgattc
     9961 gcgcggccgg cggcgcgtcg agctacaacc acctgttcgg cgccacgcgt ggcccactca
    10021 tcacgggcag cgcccgcacc ggcaagatca agaccacggc gacccagctg ctcgccaatc
    10081 gcctctatgg cgccgacatc ctggaagtcg gctacaccct gcgcgggggc atggatctgg
    10141 ctgcccacct cgaggtgtgg gacatcttgt tggccgcggg catgcgcgtg atcgccaacg
    10201 gcgccaccga caatcacaac gggagcctgt cgtcctacgc ccgcgactcc aacaacttcg
    10261 ccacggatgt gctggcaagc acctccgacc cggcgctggt gattcccgcg ctccagcagg
    10321 gacgcgcctt cgtctcgcag cacggcaagt tcggcggcct tctcgacctc agcgccaccg
    10381 gcgccgtcat ggggtcgacg gtgacgagcc ccaaatcgac ggtcaacgtg accgtgacgc
    10441 acgaaggctg ctcgggcatg agcgttcggg tgctccagta cgccgtgcat ggcagcacca
    10501 ccgtcaacgc ccgccgggcg ccactcaaga acgtctcgat caaccccggc gggcggtcca
    10561 cgaccgcact gtccttccag aacgtcaact cctatatccg gagcgaagtg tggagtggcg
    10621 gcaagcgggt cgccttcagc aacccgctgt tcatcaggcg caggtgagga gtcccgcgtg
    10681 gggagaccaa ttgtctccca atggttcttc ccaatgatcc atggcatcca caggagtgag
    10741 aaatgactaa gcggtcgctc agaaacacgg tcgcgacgat cgggtgcgcg accctcgcgc
    10801 tcgccggcct gacggccgcc cccgccgacg cagcacccgg cgacgtccag atcaacgagt
    10861 tgctctatca cgccgcggat gccgacctca cctacggcgc tctcgagttc gtcgagctgc
    10921 gcaacaccgg cagcgagccg gtggacgtcg ggggatggca gttcaccgcg ggcatcaccg
    10981 gcacggccat cccggccggc gtgaccatcc ccgccggcgg ctacctcgtc gggaccaacg
    11041 acgccgccct gttccaggcc cggttcggtt tcgccgcggg cttcgtcttc gcgaccggag
    11101 aagcgctgtc caacggcggt gagctcgtca cgctggccga tgccgccggc ggcgtcatcg
    11161 attcggtgct ctatgacgac gccgtcccat ggccgttgtc tcccgacgga gccggtccct
    11221 ccctcgaact cgatccctcc gcgacggaca acgccgccac gagctggcac gccagctccg
    11281 tcgacttcgg cacccccgcc gccgccaact cggccgcccc catggccctg accggtgtgg
    11341 gccccgactc cgtgaccccc gagcccggcg cggacgtgac cctcaccgcc acggcccccg
    11401 ccggctccac ggtgaccttg acctacaagg tcatgtatgc cgccgacgtc gtcatcccca
    11461 tggcggacga cgcggcgagc cccggcggcg ccgcggacgg cgtgtatgcc gcgacgatcc
    11521 ccggagcggg cgcaggcgaa ctcgtgcgct accgcgtcag cgcgaccaag gggacccagg
    11581 ccgcgtcata ccctgccgag ggcgattcgc gaccgtatga cgggtatgtc gtgcgcgacc
    11641 ccgaactcga ctcggccacc tatccggtcc tgcagtggtt catgtccgac gaggtctatc
    11701 aggacatgat caccaaccac cgctgcgacg actacgacgc ccaggcgacc ttcgcct
//
LOCUS       DAOPJG010000101        11616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5820_length_11616_cov_8.428856, whole genome shotgun sequence.
ACCESSION   DAOPJG010000101 DAOPJG010000000
VERSION     DAOPJG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5820_length_11616_cov_8.428856"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKU75_10100"
     CDS             <1..527
                     /locus_tag="PKU75_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPF81273.1"
                     /translation="RKWREHLYEMDYLKEGIGLRAMAQRDPLVEYQREGYNLFEAMNE
                     AIKEEAVGFLFNAEVQVQEPEPAPLQPVAIGDMLRGLDAAPADQRQPQDPALEAASAA
                     ARPEVHAKGLDEIVRPSRLHYSAPSADGGVEEHDEGSAAPELSDAQLAGARRNDPCPC
                     GSGKKFKMCHGKGA"
     gene            complement(533..1087)
                     /locus_tag="PKU75_10105"
     CDS             complement(533..1087)
                     /locus_tag="PKU75_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HPF81274.1"
                     /translation="MSTRTSKRAAAPAALPPIKVLPIPEHRPPALPADIPIEALGTGY
                     ERSMPYVQDALAFELMPRRSDVERAVAPTDLPDPHDWIGGIAQALLEVMLGLRPPSQV
                     VRSCAPDVYLAVARRHASASRRRENAATKMRRPTVAKVHSTRPDETVTESVVLVLAGP
                     RVRAMALRAEAGRTGWRVTALELG"
     gene            complement(1084..1806)
                     /locus_tag="PKU75_10110"
     CDS             complement(1084..1806)
                     /locus_tag="PKU75_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81275.1"
                     /translation="MAYGGRPARPSRVRTIATLAALAGVNALLTVITWHMGSVAMAAI
                     GAAQADPQAALIAVLATAGLAVLLAAVLDITHQCQVFARARRQLAAATTMTWRTRIAA
                     ALIGVGIGSATPAAATMPAPQTVLTAPAQPGAGATAGLPSSPAVAPDPRWGAGADTAS
                     PLIRLTRTGTDTGTGLEEVVVRRGECLWDIVARQLGPSASAAQIDAEWPRWYAANRAR
                     IGADPDLIRPGLRLVVPSDVAS"
     gene            1954..2508
                     /locus_tag="PKU75_10115"
     CDS             1954..2508
                     /locus_tag="PKU75_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81276.1"
                     /translation="MESGHRWERLFADLEAQVAAEELRERDLEIADRTRRERALTTLG
                     GRLAAHRGELVDLHLVAGIRVHGRLIDIGADWLALAPTPHRAVLVPLSAVVGVGGLGP
                     RTVEGGRARRFALGSALREISRDRRRVQVLDTAGKATEGTIDVVGADFVDLAEHPRDE
                     ARRGDNVRAARTIPFAAIACVHSE"
     gene            complement(2512..2733)
                     /locus_tag="PKU75_10120"
     CDS             complement(2512..2733)
                     /locus_tag="PKU75_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF81277.1"
                     /translation="MPKRFISLTELAEILDISAAQAYALVRSGDLPAIKIGGRGQWRV
                     ETSVLEDYIQRMYAETADFVRAQGDRDSG"
     gene            2865..3530
                     /locus_tag="PKU75_10125"
     CDS             2865..3530
                     /locus_tag="PKU75_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81278.1"
                     /translation="MSQLPEPRAKRLRRPSWRDTRLLVGVALVLLATALGAYGLRAAD
                     SRVPVYAARTQLVAGQRLTPESLQRVDVQLSDVSAGYLPVADGLPSDGYVLREVRSGE
                     LVPRAAVGSGAEVAVSPLSIVVDQASAGALVVGSTVDVYASTPKADKSSGSGYDAPRA
                     VLESVQVARLSSGSGFGSSSRSSAVQVLVPREHIAALITLVDAEAHFTLVPVPGSALR
                     EAS"
     gene            3527..4885
                     /locus_tag="PKU75_10130"
     CDS             3527..4885
                     /locus_tag="PKU75_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81279.1"
                     /translation="MTVRILTGVEPRHEALVARHLASLPGVELVRRCPDLADLVAAAS
                     AGLADAAIVSAGLRGIDRDTLGHLRDCGVVAVGLVDDEEQERRLRQLGVAATLPADCT
                     AEEIDAVLTRSLGRGSAEAGAVSDEEWAALEAGLDPAGAPAPPDTQDPRERAGRGRLV
                     VVWGPAGAPGRTSVAINLAGELALTGRSVLLIDADTYGASIGQALGLLDEAPGLAAAA
                     RAAELGTLDVPTLARLSPVVEPGFRVLTGLPSASRWPEVRADSMGHLLDLARALSQVV
                     VVDIGFCLEDDEELSYDTRAPRRNAVAVTTLAEANAVVAVGAGDPVGLQRLVRALPDV
                     QAVSPRVPTVVINKVRAGVAGASPAKRICEALDRFAAVSVEHVIPEDQGAFDKALLAG
                     RLLSDVAPKSAARSRIRDIALAVAPDRAVAVAPAAAGARQGKGSFGRLAAVRPSWRQG
                     TS"
     gene            5030..8335
                     /gene="fdh"
                     /locus_tag="PKU75_10135"
     CDS             5030..8335
                     /gene="fdh"
                     /locus_tag="PKU75_10135"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5597..5599,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HPF81280.1"
                     /translation="MADRGTFLGWPVLRQLASGDFLGRGPAVTTRHTRERTARTATAD
                     KVVKSVCPYCAVGCGQNIFVKDGRVVQIEGDPDSPVSRGRLCPKGSASEQLVNNPRRQ
                     TTVLYRAPYATDWTHLGLDEATDMIADRVVETRRRTWQDKDEHGQFLRRTMGIASLGG
                     AALDNEENYLMKKLYTAMGAIQVENQARIUHSSTVPSLGASFGRGGATQFVQDMANSD
                     CIVIQGSNMAECHPVAYQWVTEAKLRGARVIHVDPRFTRTSAVADRHIPIRAGSDVIL
                     LGALINHVLTTETYFRDYVQAYTNATMLVSDDFQDTEDLGGVFSGYDPETGQYDLTSW
                     AYKSRAAGEEKVGEPGPEEHGAEQAAVAEGQAYGSGGPPLDHALIERDPTMTDPRCVL
                     QVLRRHYARYTPALVTEVCGIGREDFDYLARSIVENSGRERTTMFAYAVGWTQHSLGA
                     QFIRTSAILQLLMGNVGRPGGGIMALRGHASIQGASDIPTLFNLLPGYLPMPVAGKHE
                     SLGQYLEAVSSKRQKGFWTKADTYMVSLLKAWWGDAAQPDNDFCYDYLPRLNGNHNTY
                     QSVMDMIAGTVKGYFLLGQNPAVGSAHGRMQRFGMANLDWLVVRDINLIESATWWKDG
                     PEIASGEMETDKIATEVFFLPAANHAEKDGTFTQTQRMLQWHFKAVEPPGDARSDLWF
                     YYVLGQKIRERLAGSTDPVDRPLLDLTWDYPQDEHGEPDADAILREVNGTYLTGEKAG
                     QALPGFVELKADGSTASGCWIYSGVYAGEVNRSKNKVPDAEQSWVAPDWGWAWPMNRR
                     ILYNRASADPQGRPWSERKAYVWWDEEQGTWVGHDVPDFPVTTDPAYRPADTATGPDA
                     LAGDDPFIMQADGKGWLFAPKGMIDGPMPTHYEPQESPVDNALYPQQRNPSRLVYPRK
                     DNLTAPSAGDPGVDVYPYVATTYRLTEHHTAGAMSRWLPYLSELQPEFFCEVSPELAA
                     ERGLENGGWATLISPRSAIEARVLVTERMTPLVVGGRTVHQIGMPYHWGVGGTGAVVQ
                     GDSANDLLGVTLDPNVSIQESKVSSVDIQPGRRPHGADLLRLVQDYQSRSGITVMTGN
                     DRLEDAEEREEAR"
     gene            8395..9597
                     /locus_tag="PKU75_10140"
     CDS             8395..9597
                     /locus_tag="PKU75_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPF81281.1"
                     /translation="MDAHFWKNQLSGPTDPAQDAAWPDPPPRKGFFTDTSICIGCKAC
                     EVACKEWNQLPMDNIGMTGNSYDNTGQLGANTWRHVAFVEQSAEHIAQARVAGARLVE
                     LGMPRVRDAAVGSPDAGLPRADAGLPRATAMNSSRAVPSGLPLDGPGTRAALPSESSE
                     RDTPTNGLDPGRVALTRPGEEDASAMQWAAEQALAEGTVPDFRWLIASDVCKHCTHAG
                     CLDVCPTGALFRTEFGTVVVQDEVCNGCGYCVAACPFGVIDKRKDPTTTGAARQVEIG
                     IANKCTLCYDRISLDAKPACAQACPTTSIQFGDLDEMRERARERVEALHANGFTEARL
                     YGANPNDGVGGTGSVFLLLDEPEVYGLPPDPIVTTHDLPDIFRKAGLAGLTMLAGAAV
                     SFLGRGNS"
     gene            9642..10748
                     /gene="nrfD"
                     /locus_tag="PKU75_10145"
     CDS             9642..10748
                     /gene="nrfD"
                     /locus_tag="PKU75_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPF81282.1"
                     /translation="MPQPDTDAGESGAVTATAAAPVRRRRRRRDDIMVPDAAFDSYYG
                     RSVVKPAPWDYKIPTYLYSGGLAAGSALVAAGAEMTGRTLLQRNARLTALGALGVSTV
                     ALVADLGRPSRFVNMLRVMKLTSPMSIGSWILAGFGAFTGVAAASEVAKFVIKPDSAI
                     NRYLPIVDRGASIGAGFFSAPLAAYTAVLLSNTATPTWHGIYRELPFAFVGSGMAAAG
                     GAAMVTTTTRQTAPARRMALGGAALELTAFRLLEHNAGLVGEPLRQGKAGQLLGASTA
                     LSLTGIGLTVLAGRWRPAAVLAGLAFNGASATLRFGVFHAGIESAKDPKYTVVPQKER
                     IAAREAQAALSWSSNASTPGALRPPTSQSPQELA"
     gene            10893..11066
                     /locus_tag="PKU75_10150"
     CDS             10893..11066
                     /locus_tag="PKU75_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81283.1"
                     /translation="MVAAIPFEVAIGMIFVGAFIGGKIQDRRGPRTVALVGVVIYAIG
                     IILSSFARERSDL"
     gene            11067..>11616
                     /locus_tag="PKU75_10155"
     CDS             11067..>11616
                     /locus_tag="PKU75_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81284.1"
                     /translation="MLILGYGVLSGFGLGMAYIVPIAMLQKWFSDKAGLITGIAVGGC
                     GAVITSPVAGALIANDKAYPTAPLRILGIAYLIVGVLGALLFANPPKGYRPPGMAAPA
                     ATPTGSATVAQTGVRDFTQPEALRTPQWYLLTAILALSVTAGISLISQAAGAGSAIAG
                     MSAASASTFVGIMGLFSGGRSRG"
BASE COUNT         1741 a         4119 c         4060 g         1696 t
ORIGIN      
        1 accgcaagtg gcgcgagcac ctctacgaga tggactacct caaggagggc atcgggctgc
       61 gggcgatggc ccagcgcgac ccgctcgtcg aataccagcg cgagggctac aacctgttcg
      121 aggccatgaa cgaggcgatc aaggaggagg ccgtcggctt cctcttcaac gccgaggtcc
      181 aggtccaaga gcccgaaccg gcaccgctgc agccggtcgc catcggcgac atgctccgcg
      241 gcctggacgc cgccccggcc gaccagcggc agccgcagga ccccgccctc gaggcagcct
      301 cggctgcagc ccgcccggag gtgcacgcca agggactgga cgagatcgtg cgcccctcac
      361 gcctgcacta ctccgctccc agtgcggacg gcggcgtcga ggaacacgac gagggcagcg
      421 ctgcccccga actctccgac gcccaactcg ccggcgcccg ccgcaacgat ccctgcccct
      481 gcggctcggg caagaagttc aagatgtgcc acggcaaggg cgcctgagcg gctcagccca
      541 gttcgagggc ggtgacgcgc cagccggtcc tgccggcctc ggcacgcaac gccatcgccc
      601 gcacccgggg tccggcaagg acgagcacga cgctctcggt gacggtctcg tccggacgcg
      661 tgctgtgcac cttcgcgacg gtcgggcggc gcatcttggt ggcggcgttc tcccgccgcc
      721 gggaggccga tgcgtgacgt cgcgcgacgg cgaggtaaac gtcgggcgcg cacgagcgca
      781 ccacctgtga cggcggccgc aagcccagca tcacttcgag cagggcctgg gcgatcccgc
      841 cgatccagtc gtgcggatcg ggtagatcgg ttggtgccac cgcccgctcc acgtcgctgc
      901 gacgcggcat caactcgaag gccaaggcgt cctggacata gggcatgctg cgctcgtagc
      961 ccgtgccgag tgcctcgatc gggatgtcgg cgggcagcgc cggcggccgg tgttcgggga
     1021 ttggcagcac cttgatgggt ggcaatgccg ccggcgctgc ggcgcgcttc gacgtccggg
     1081 tgctcatgac gccacgtccg acgggacgac caagcgcaaa cccggacgga tcaagtcggg
     1141 gtcggcgccg atgcgggcgc ggttcgcggc ataccagcgg ggccattcgg cgtcgatctg
     1201 tgcggcggag gcgctcgggc cgagctggcg ggcgacgata tcccacaggc actcgccccg
     1261 gcggaccacg acctcctcga ggccggtgcc cgtgtcggtg ccggtgcggg tcagccggat
     1321 cagcggcgag gcggtgtcgg cgccggcccc ccaacgcgga tcgggtgcca cggccgggct
     1381 ggaggggagc ccagccgtag cacccgcccc cggctgggcc ggggccgtga ggaccgtctg
     1441 cggcgccggc atcgtcgccg cggccggtgt cgcgctgccg attcccacgc cgatcagggc
     1501 cgcggcgatc cgcgtgcgcc acgtcatcgt cgtcgcagct gccagctgcc gacgggcgcg
     1561 ggcgaacacc tgacattgat gcgtgatgtc caagacggcg gcgagcagga cggcgagtcc
     1621 cgccgtagcc agcacggcga tgagcgccgc ctgcgggtca gcctgcgccg cgccgatcgc
     1681 ggccatggcg accgacccca tgtgccaggt gatgaccgtg agcagcgcat tgacccccgc
     1741 cagggcggca agggtcgcga tcgtgcggac ccggctcggt cgtgccggac gccccccgta
     1801 tgccatgact cctcctgcag gtgatgcgcc agatcgcgcc acgatgatgt cgtttggtga
     1861 agtttgctgc agtttgagac actctgcaac agatagccgc tgatcctgtg gagaacgcgc
     1921 agcgaccgcc cgctctacct acgattgggc ggcatggagt ccggccaccg gtgggagcgg
     1981 ctcttcgccg acctggaggc gcaggtcgcg gcggaggaat tgcgcgaacg cgatctcgag
     2041 atcgccgacc gcacccgccg tgagcgtgcg ctgaccacgc tcggcggacg gctggccgcg
     2101 caccggggcg agctggtcga cctgcacttg gtcgccggga tccgggtgca tggccgcctg
     2161 atcgacatcg gagctgactg gctcgccctc gcccccacac cacatcgcgc cgttctggtc
     2221 ccgctgagcg ccgtcgtcgg cgtcggcggt ctggggccgc ggaccgtcga aggcggccgc
     2281 gcccggcgct tcgccctggg ctcggcgttg cgcgagatca gccgggaccg gcgccgcgtg
     2341 caagtcctcg acaccgcggg caaggcgacc gaaggcacca tcgacgtcgt cggcgccgat
     2401 ttcgtcgacc tcgccgagca cccccgcgac gaggcgcgac ggggcgataa cgtccgtgcc
     2461 gcgcgcacga tccccttcgc cgccatcgct tgcgtgcaca gcgaataagg gtcagccgga
     2521 gtcgcgatcc ccctgggcgc gcacgaagtc ggccgtctcg gcatacatcc gttggatgta
     2581 gtcctcgagc acgctcgtct ccacccgcca ctgtccgcgt ccgccgatct tgatggcggg
     2641 cagatcgccc gagcgcacca gcgcgtaggc ctgggcggca gagatgtcga ggatctccgc
     2701 gagctcggtg agcgagatga atcgcttcgg catgccccga ttctgccgca ccgcgaccgg
     2761 tcgaccgtcg cgtgttatcc ccagccgcgt cgtttcaaga tgccgactcg cgcgctgtgg
     2821 gtcatagtgg ctgcacacat caggcccgtg ggggggagac actcatgtcg caattgccgg
     2881 aaccgcgtgc gaagcgcctg cgccgcccgt cgtggcgcga cacgcgcctg ctcgtcgggg
     2941 tggctctggt cctgctggcg accgcgctgg gggcttatgg tctgcgcgcc gcggattccc
     3001 gcgttccggt gtatgccgcg cgcacccagc tcgtcgccgg tcagcgcctg acgccggagt
     3061 cgctgcagcg cgtggacgtc cagttgtcgg acgtgtcggc cggttatctt ccggtcgccg
     3121 acgggctgcc gtcggacggg tatgtgctac gggaggttcg ttcgggtgag ttggtgccgc
     3181 gcgcggccgt gggcagcggc gcggaggtcg ccgtctcgcc gctgtcgatc gtggtagatc
     3241 aagcctccgc gggggccctg gtcgtcggct cgacggtgga tgtctatgcg agcactccca
     3301 aggcggacaa gagctccgga tccgggtatg acgccccgcg agcagtgctc gagtcggtgc
     3361 aggttgcccg actcagttcg gggtcgggat tcggctcctc cagcaggagc agtgccgtcc
     3421 aggtcctggt gccgcgcgag cacatcgccg ccctgatcac cctggtcgac gccgaggcgc
     3481 acttcaccct ggtgcccgtg cccggctcgg ccctgcggga ggcttcgtga ccgtccgcat
     3541 cctcaccggc gtcgaaccgc gccacgaggc gctcgtcgcc cggcacctcg cgagtctgcc
     3601 cggggtcgaa ctcgtccgcc gttgccccga cctcgccgat ctcgtcgcag ccgcgtcagc
     3661 ggggctcgcc gatgccgcga tcgtgtccgc cggcctgcgt gggatcgatc gggacacgct
     3721 tggtcacctg cgcgattgcg gtgtggtggc tgtcggcctg gtcgatgacg aggagcagga
     3781 gcgacggctg cgtcagctcg gggtggccgc gaccttgccg gcggactgca cggccgagga
     3841 gatcgacgcc gtgctcaccc gctcgctcgg ccgagggtcg gccgaggccg gggcggtctc
     3901 cgacgaggag tgggcggccc tcgaggcggg cttggacccg gcgggcgctc ctgcgccgcc
     3961 cgacacgcag gatccgcggg agcgggccgg ccgcggccgg ctggtggtcg tatggggtcc
     4021 ggccggggcg ccgggccgca ccagcgtcgc catcaacctc gccggcgaac tggcgctgac
     4081 cggccggagt gtcttgctca tcgacgccga cacctatggc gcgagcatcg gacaggcctt
     4141 gggtctgctc gacgaggccc ccggcctcgc ggcggcggcc cgggccgctg agctcgggac
     4201 tctcgacgtg ccgacgctgg cgcgcttgtc gccggtcgtc gagccgggat tccgcgtgct
     4261 gaccgggttg ccgagtgcga gccggtggcc cgaagtgcgt gccgactcga tgggtcacct
     4321 gctcgacctg gcccgtgcgc tctcgcaggt cgtggtcgtc gacatcggtt tttgcctcga
     4381 ggacgacgag gaactcagct acgacacgcg ggcgccccgg cgcaacgccg tggccgtcac
     4441 cacgctggcg gaggccaacg ccgtggtggc ggtcggagcg ggtgatcccg tcgggctgca
     4501 acgacttgtc cgtgccctgc cggacgtgca ggcggtcagc ccgcgcgtgc ccacggtcgt
     4561 gatcaacaag gtccgtgccg gcgtcgccgg cgcctccccg gccaagcgca tctgcgaggc
     4621 cctcgaccgg ttcgccgccg tcagcgtcga gcacgtcatt cccgaggatc agggtgcctt
     4681 cgacaaggcg ctgctcgccg gacggctgct gtccgacgtc gcccccaaga gcgccgcccg
     4741 ctcccggatc cgcgacatcg cgctcgccgt cgcgccagat cgggccgtgg cggtggcgcc
     4801 ggccgccgcg ggcgcccgcc agggcaaagg atccttcggc cggctcgcgg ccgtgcgccc
     4861 ctcgtggcga cagggcacct cctgatccgc gactgctaat ccgcgattgc ggcggcgggt
     4921 gccgataggg tccctgggca gggcgaatgc gcaggacgga tgcgtaggaa gatgcacagg
     4981 cccgatgcat caggacggcg cgtcagaccc aaccgagcag gagggtgcga tggcggatcg
     5041 aggcacattc ctcggctggc cggtgctgcg gcagctggcg agcggcgact tcctgggccg
     5101 cgggccggcg gtgaccacgc ggcatacccg cgaacggacg gcacgcaccg caaccgctga
     5161 caaggtcgtc aaatccgtct gcccctattg cgccgtcggc tgcgggcaga acatcttcgt
     5221 caaggacggc cgggtcgtgc agatcgaggg cgaccccgac agtcccgtca gccggggccg
     5281 gttgtgcccc aagggcagcg ccagcgagca gctggtcaac aacccccgcc gccagaccac
     5341 cgtcctgtat cgcgcgccct acgccaccga ctggacacac ctcggcctgg acgaggcgac
     5401 cgacatgatc gccgaccggg tggtcgagac ccgccggcgc acctggcagg acaaggacga
     5461 gcacggccag ttcctgcgcc gcaccatggg catcgcgagc ctgggcgggg ccgcgctgga
     5521 caacgaagag aactacctga tgaagaagct gtatacggcc atgggcgcga tccaggtcga
     5581 gaaccaggcg cgtatttgac acagctccac ggttcccagt ctgggagcct cgttcgggcg
     5641 cggcggcgcc acgcagttcg tgcaggacat ggccaattcg gactgcatcg tcatccaagg
     5701 ctccaatatg gccgagtgcc acccggtggc ctatcagtgg gtgacggagg cgaagctgcg
     5761 tggtgcccgg gtgatccacg tcgacccgcg tttcacccgc acctccgccg tggccgaccg
     5821 gcacatcccc atccgcgccg gcagtgacgt catcctcctc ggtgccctga tcaaccacgt
     5881 gctgaccacc gagacctact tccgcgacta cgtccaggcc tacaccaacg ccacgatgct
     5941 cgtctccgac gactttcagg acaccgagga tctcggcggc gtcttctccg gctacgaccc
     6001 cgagaccggg cagtacgacc tgaccagctg ggcctacaag tcccgggcgg cgggggagga
     6061 gaaggtcggc gagcccggcc cggaggaaca cggcgccgag caggcagcgg tggccgaggg
     6121 acaggcatac ggctcgggcg gcccgccgct cgatcacgcg ctcatcgagc gcgacccgac
     6181 gatgaccgac ccgcggtgcg tgctgcaggt gctgcggcgc cattacgcgc gctatacccc
     6241 cgcactcgtc accgaggtct gcggcatcgg gcgcgaggac ttcgactatc tcgcccgctc
     6301 gatcgtcgag aacagcggcc gggagcggac gacgatgttc gcgtatgcgg tgggctggac
     6361 ccagcattcc ctcggggccc agttcatccg caccagcgcg atcctgcagc tgctcatggg
     6421 caatgtcggg cggccgggcg gcgggatcat ggcgctgcgc ggtcacgcct ccatccaggg
     6481 agccagcgac atcccgaccc tgttcaacct gctgccgggc tatctgccga tgccggtggc
     6541 gggcaagcac gagtccctcg ggcaatacct cgaggccgtt tcctccaagc ggcagaaggg
     6601 tttttggacc aaggccgaca cgtacatggt cagcctgctc aaggcgtggt ggggcgatgc
     6661 ggcgcagccg gacaacgact tctgctacga ctacctgccc cggctgaacg gcaaccacaa
     6721 cacctatcag tcggtcatgg acatgatcgc cggcacggtc aagggctatt tcctcctcgg
     6781 tcagaacccg gccgtcgggt ccgcccacgg ccggatgcag cgtttcggca tggccaatct
     6841 cgactggctc gtcgtgcgag atatcaacct catcgagtcg gcgacctggt ggaaggacgg
     6901 ccccgagatc gcctccggcg agatggagac cgacaagatc gccaccgagg tcttcttcct
     6961 tccggcggcc aatcacgcgg agaaggacgg caccttcacc cagacccagc ggatgctgca
     7021 gtggcacttc aaggccgtcg agccgccggg cgacgcccgc tccgacctgt ggttctacta
     7081 cgtcctcggc cagaagatcc gcgagcggct cgccggctcc accgacccgg tcgaccggcc
     7141 cttgctcgac ctgacctggg actatccgca ggacgagcac ggtgagccgg acgccgacgc
     7201 gatcctgcgc gaggtcaacg gcacctatct gaccggggag aaggccgggc aggcgctgcc
     7261 cggtttcgtc gaactcaagg ccgacggctc gaccgcctcc gggtgttgga tctattccgg
     7321 cgtgtatgcc ggtgaggtca accggtccaa gaacaaggtc cccgacgccg agcagagctg
     7381 ggtcgcaccc gactggggct gggcctggcc gatgaaccgg cgcatcctct acaaccgcgc
     7441 gtccgccgac ccgcagggcc ggccgtggag cgaacggaag gcctacgtct ggtgggacga
     7501 ggaacagggc acgtgggtgg gccacgatgt gcccgacttc ccggtgacca cggacccggc
     7561 ataccgcccg gcggacaccg cgaccggacc ggacgccctc gccggcgacg atccgttcat
     7621 catgcaggcc gacggcaagg gctggctatt cgcgcccaag ggcatgatcg acgggccgat
     7681 gccgacccac tacgagccgc aggagagccc ggtcgacaac gccctctatc cccagcagcg
     7741 caatccgtcg cggctggtct atccgcgcaa ggacaacctc acggcgccgt cggcggggga
     7801 tcccggtgtc gatgtctatc cgtatgtcgc gacgacctac cggctgaccg agcatcacac
     7861 cgcgggggcg atgagccgct ggctgccgta tctgtcggag ctgcagccgg agttcttctg
     7921 cgaggtcagc cccgagctcg ccgccgaacg cggcctcgag aacggcgggt gggcgacgct
     7981 gatctcgccg cgctcggcga tcgaggcgcg ggtgctggtg acggagcgga tgactccgct
     8041 ggtcgtcggc gggcgcaccg tgcaccagat cggtatgccg tatcactggg gcgtcggcgg
     8101 caccggcgct gtggtgcaag gagattcggc caacgacctg ctcggggtca ccctcgaccc
     8161 caacgtctcg atccaggaga gcaaggtcag ttcggtcgac atccagccgg ggcggcgccc
     8221 gcacggggcc gacctgctgc ggctggtgca ggactatcag tcccggtccg ggatcacggt
     8281 gatgaccggc aacgaccggc tcgaggacgc cgaggagcgt gaggaggccc ggtgagcatc
     8341 tcgacgtcgc ccgccgaatg gggcaaggga gtgcgctcga tgctgcgcga cagcatggac
     8401 gcccacttct ggaagaacca gctgagcggc cccaccgatc ccgcgcagga cgccgcgtgg
     8461 ccggacccgc cgccgcgcaa ggggttcttc accgacacct cgatctgcat cggctgcaag
     8521 gcgtgcgagg tggcgtgcaa ggagtggaac cagctgccga tggacaacat cggcatgacc
     8581 ggcaactcct acgacaacac cggccagctc ggcgccaaca cctggcggca tgtcgccttc
     8641 gtcgagcagt cggccgagca catcgcgcag gcccgggtcg ccggggcccg cctcgtggag
     8701 ttgggtatgc cgcgggtgcg ggatgccgct gtcgggtcgc cggacgctgg gctcccgcgg
     8761 gcggacgctg ggctcccgcg ggcgacggcg atgaactcca gccgggcggt cccgagcggg
     8821 ttgccgctcg acggcccggg cacccgcgcc gccctgccct ctgagtcgag tgagcgcgac
     8881 acgcccacaa atgggctcga tcctgggcgt gtcgcgctca ctcgaccggg ggaggaggac
     8941 gcctcggcga tgcagtgggc cgccgaacag gccctggcgg agggcaccgt cccggacttc
     9001 cgctggctca tcgccagcga cgtctgcaaa cactgcacgc acgcgggctg cctggatgtc
     9061 tgcccgaccg gcgcgctctt tcgcaccgag ttcggcacgg tggtcgtgca ggacgaggtg
     9121 tgcaacggct gcggctactg cgtggccgcc tgccccttcg gcgtcatcga caaacgcaag
     9181 gacccgacca cgacgggcgc ggcacgtcag gtcgagatcg gcatcgccaa caagtgcacc
     9241 ctgtgctacg accggatctc tctcgacgcc aagccggcgt gcgctcaggc gtgcccgacg
     9301 acgtcgatcc agttcggcga cctggacgag atgcgcgagc gcgcccggga gcgcgtggaa
     9361 gcgttgcacg ccaacggttt caccgaggcg cggctctatg gggccaaccc caacgacggg
     9421 gtcgggggca ccgggtcggt cttcctgctg ctggacgagc cggaggtcta tggcctgccg
     9481 ccggacccga tcgtgaccac gcacgacctg cccgacatct tccgcaaggc cggcctcgcc
     9541 ggcctgacga tgctcgccgg agccgcggtg agcttcttgg gacgaggcaa ctcatgagtg
     9601 acgcggagga gcagagagcc gccggaaaca cgaacggccc tgtgccgcag cctgatacgg
     9661 acgcggggga gtcgggggcg gtgaccgcga cagcggccgc gccagtgcgc cgtcgccggc
     9721 ggcgacgcga cgacatcatg gtgcccgacg cggcgttcga ctcctactac gggcggtcgg
     9781 tggtcaaacc cgcgccatgg gactacaaga tccccactta tctctacagc ggcggtctgg
     9841 cggcgggctc ggcgctcgtc gccgccggcg cggagatgac gggccggact ctgctgcaac
     9901 gcaatgcgcg gctgacggcg ctcggcgcgc tcggcgtgag caccgtcgcg ctcgtcgccg
     9961 acctcggccg gccctcgcgg ttcgtcaaca tgctccgggt gatgaagctg acctccccga
    10021 tgagcatcgg cagctggatc ctcgccggat tcggcgcctt caccggggtc gcggccgcgt
    10081 ccgaggtggc gaagttcgtc atcaaacccg acagcgcgat caaccgctac ctgccgatcg
    10141 tcgaccgcgg cgcgagcatc ggtgcgggat tcttctcggc cccgctcgcg gcatacaccg
    10201 cagtcttgct gtccaacacc gcgaccccga cgtggcacgg gatctatcgc gaactgccct
    10261 tcgccttcgt cggctccggc atggccgccg ccggcggcgc cgcgatggtc acgacgacga
    10321 cgcgacagac cgcgccggcc cggcggatgg ccctcggcgg cgccgcactg gagttgaccg
    10381 ccttccggct gctggagcac aacgccgggc tcgtggggga gccgctgcgg cagggcaaag
    10441 ccgggcagct gctgggggcg agcaccgcgc tcagcctcac cggcatcggc ctgacggtgc
    10501 tcgcgggccg ctggcggccg gcggcggtgc tcgcaggcct ggccttcaac ggggcgtcgg
    10561 ccaccttgcg gttcggggta ttccacgccg gcatcgaatc ggccaaagac ccgaaataca
    10621 cggtggtgcc gcagaaggag cgcatcgccg cccgggaggc gcaggccgcg ctgtcctggt
    10681 cctccaacgc ctcgactccc ggcgccctcc gtccccctac gtcgcagtcc ccccaggagt
    10741 tggcatgacc gaaaccgttg caacgacgac gaaagccgct ggctgatcct gctcggcggc
    10801 gtgctcgtcc agctggccat cggcgccgtc tatgcctgga gcaccttcag caaggcgttc
    10861 gtcgcgcccg agtcggcgat gcacctgacc aagtggtcgc ggccatcccc ttcgaggtcg
    10921 ccatcgggat gatcttcgtc ggggccttca tcggcggcaa gatccaggac cgccgcgggc
    10981 cacggacggt cgcactcgtc ggtgtcgtca tctatgccat cggcatcatc ctcagctcct
    11041 tcgcccgcga gcgcagcgac ctgtagatgc tcatcctcgg ctacggcgtg ctcagcgggt
    11101 tcggtctcgg catggcctat atcgtcccga tcgccatgct gcagaagtgg ttttccgaca
    11161 aggccggcct gatcaccggc atagccgtcg gcggctgcgg cgccgtcatc acctcgcccg
    11221 tcgccggcgc gttgatcgcc aacgacaagg cctacccgac cgcgcccttg cgcatcctcg
    11281 gcatcgccta cctgatcgtg ggcgtcctcg gcgcgctgct gttcgccaac ccgcccaagg
    11341 gctaccgccc acccggcatg gccgcaccgg cggcgacacc caccgggagc gcgaccgtcg
    11401 cccagaccgg ggtgcgcgac ttcactcaac cggaggcact gcgtaccccg cagtggtatc
    11461 tgctgaccgc gatcctcgcc ctgtcggtca cggccggcat ctcgctcatc tcccaagccg
    11521 ccggagccgg ctcggccatc gccggcatga gtgccgccag cgcctcgact ttcgtcggga
    11581 tcatgggcct gttcagcgga ggccgtagcc gaggat
//
LOCUS       DAOPJG010000102        11572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5847_length_11572_cov_8.576626, whole genome shotgun sequence.
ACCESSION   DAOPJG010000102 DAOPJG010000000
VERSION     DAOPJG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5847_length_11572_cov_8.576626"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PKU75_10160"
     CDS             complement(<1..1077)
                     /locus_tag="PKU75_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HPF81285.1"
                     /translation="MTGDHLPTAYCCSIVPPHILERLAHSADAEIADAARQALRDVAE
                     GMLHRRAHAAPESRPGSKPRLAPGTLEGGPQRLICDAQGGTTLPGTQVRGEGEPDTGD
                     VAVAEAYDGLGDTWQLFADAFERNSLDGRGLPLRATVHYGENYDNAFWDGTQMVFGDG
                     DGKIFGRFTASLDVIGHELAHGVTEHTAGLMYQGQPGALNESMSDVFGSMVKQRRLGQ
                     GAADADWLIGAELLIGELAGQALRSMKAPGTAYDSPLLGKDPQPGHMDDYVETDDDHG
                     GVHINSGIPNRAFYLLATALGGASWEAPGRIWYAVLTGPGISADCDFVTFAGLTVDEA
                     IGQYGAQSPEVAAVKSAWAQVGVLG"
     gene            complement(1122..1631)
                     /locus_tag="PKU75_10165"
     CDS             complement(1122..1631)
                     /locus_tag="PKU75_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HPF81286.1"
                     /translation="MSDENLAPSDPDAPAEALARARALARAKGLRPGSMPKRAPRGGA
                     PESGRTDRDPALLGDVVDRLVSDRGWEREVEVGAVVGRWEQIVGAEIAAHASAESFAD
                     GVLVVRASSTSWATQLRLLSSSLLARIAAEAGPDTVTELQIIGPTAPRWSHGRRGITG
                     GRGPRDTYG"
     gene            complement(1624..2835)
                     /gene="recF"
                     /locus_tag="PKU75_10170"
     CDS             complement(1624..2835)
                     /gene="recF"
                     /locus_tag="PKU75_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPF81287.1"
                     /translation="MFVRHLSLIDFRSYPAAEVPLGPGVTTLVGLNGQGKTNIAEALG
                     YLATLSSHRVSTDQPLVRFGASQAIVRGGVVRDGRETLVELEINPGKANRARLNRSPV
                     PRPREVLGQVRSVLFAPEDLALVKGDPAARRRFLDELLVQRQPRWAAVRADYDRIVKQ
                     RSALLKQSAPMLRRGGRGRRPAEGASAEEARESVLHTLHVWDGHLAGLGAQLLYARLR
                     LLRDLRTHLDHAYREVSGGAGEALATYRASLTQGLNARIAAGEVPEIDELSAGIVAAL
                     AEVQANEFERGQCLVGPHRDDLVLALGEMPAKGYASHGESWSFALGLKLAAYQLLRTD
                     LGTDPVLILDDVFAELDSGRRERLAALIGDCEQVLITAAVADDIPKSLAGQWFSVTKG
                     EITAYPEAPDE"
     gene            complement(2923..4050)
                     /gene="gnd"
                     /locus_tag="PKU75_10175"
     CDS             complement(2923..4050)
                     /gene="gnd"
                     /locus_tag="PKU75_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HPF81288.1"
                     /translation="MQLGLIGLGKMGGNMRERIRRAGHEVIGYDINPKVSDVKSLAEL
                     VKSLEAPRVVWTMVAAGEITRSTVAKLADLLEPGDLVIDGGNSRFTDDFENEKLLKAK
                     GIGYMDCGVSGGIWGLENGYGLMCGGTAANVRRAMPIFDALRPEGPRDEGFVHAGKVG
                     AGHYTKMVHNGIEYGLMHAYAEGYELLEKKDIVTNVPGAFKAWSRGTVVRSWLLDLMV
                     KALEENPTLENVSDYTADSGEGRWTVEEAIALSVPMPVISASLFARFASRQEHSPTMQ
                     AVAALRGQFGGHQVMTVAEGDKLREEGAAAPASPRAVKKRTVPKEKPTKSAANAAAKG
                     RKAAAAGPSSGSGSTQGGSGAAGAGPTSGTGSTRKRAAKRA"
     gene            complement(4278..5414)
                     /gene="dnaN"
                     /locus_tag="PKU75_10180"
     CDS             complement(4278..5414)
                     /gene="dnaN"
                     /locus_tag="PKU75_10180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPF81289.1"
                     /translation="MKFRVERDVLAEAVTWVARGLPMRPAVPVLAGVLIDAGTDGTVT
                     LSAFDYEVSARITVSAEVAEPGTVLVLGKLLSEIARNLPADKPVDIATDGSKVQVTCG
                     SSRFALLQMPASDYPTLPTQPEASGTIDGATFAQAVSQVAVAADRGDTLPILTGVRVE
                     IEGDKVTFLATDRYRLAMREFTWNPAAADASHIALIPARTLSETARSLGSGGSVAVAL
                     GQAAGGDGLVGFEAGQRHSTSRLLDGEYPKVAKIFPATSEIEAVVKTSDLIEAVKRVA
                     LVAERNTPVRLKFTSGQVDIEAGTGDDAQASEAVEATLDGADIDIAFNPGFLLDGLGA
                     LHTPYARFSFTAALKPAMLTGQAEVDGSPELSYRYVLQPVRLTS"
     gene            complement(6024..7454)
                     /gene="dnaA"
                     /locus_tag="PKU75_10185"
     CDS             complement(6024..7454)
                     /gene="dnaA"
                     /locus_tag="PKU75_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPF81290.1"
                     /translation="MAEASMDTGALWNSVLTTLAQDGIPMNQRAFLQGAKVKGVLDQT
                     VLVAVSNDFAKQILEQRVREHVIGVLGRELGGEVTLAVTVDPSLATEDDLFSSTADLA
                     EPVEVVEEDEEIEELPAAPVTYLPDAEDIGATRLNPKYVFDTFVIGSSNRFAHAAAVA
                     VAEAPAKAYNPLFIYGGSGLGKTHLLHAIGHYARSLYPQLRVRYVNSEEFTNDFINSI
                     RDDRAANFQSRYREVDVLLIDDIQFLSGKVQTQEEFFHTFNSLHNANKQVVITSDVQP
                     KHLSNFEDRMVSRFEWGLLTDVQPPDLETRIAILRKKAIQERLSVPPEVLEYIASRIS
                     TNIRELEGALIRVTAFASLNRQAVDLPLAEIVLKDLIPNEHTNQISSATIMAQTAAYF
                     GLTIDDLQGASRSRVLVTARQIAMYLCRELTDLSLPKIGQQFGGRDHTTVMHADKKIR
                     QLMAERRAIYNQVTELTNRIKQQSAS"
     gene            7911..8048
                     /gene="rpmH"
                     /locus_tag="PKU75_10190"
     CDS             7911..8048
                     /gene="rpmH"
                     /locus_tag="PKU75_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPF81291.1"
                     /translation="MSKRTFQPNNRRRAKNHGFRARMSTRAGRAILAARRRKGRAELS
                     A"
     gene            8064..8429
                     /gene="rnpA"
                     /locus_tag="PKU75_10195"
     CDS             8064..8429
                     /gene="rnpA"
                     /locus_tag="PKU75_10195"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPF81292.1"
                     /translation="MLPAQHRLRASGDFAAVLRRRGRVSAAARVLVVHANAIPGREDL
                     PSRVGFVVGKSVGNAVVRGRTKRRLREHCRSRLGLLMPGTDYVVRAQPAAAGATFAEL
                     GAALDEALARLGPRWSRTA"
     gene            8537..8875
                     /gene="yidD"
                     /locus_tag="PKU75_10200"
     CDS             8537..8875
                     /gene="yidD"
                     /locus_tag="PKU75_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPF81293.1"
                     /translation="MWPIRFYQRFISPATPPTCRYYPTCSGYAVTALERFGPVKGFYL
                     AARRLLRCHPWAPGGVDHVPETFTWRRASGIPEAPPQADPHEHGTFPVTGTFDTTAPL
                     PAQDTPPKAS"
     gene            8884..9945
                     /gene="yidC"
                     /locus_tag="PKU75_10205"
     CDS             8884..9945
                     /gene="yidC"
                     /locus_tag="PKU75_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPF81294.1"
                     /translation="MGDLFNSILYPIEWIVAWIMYGFHQAFTAIGMPAASGWTWALSI
                     VGLVIVMRALMIPLFVKQIHASRKMQMISPELQKIQAKYKGKTDPESRQKMTQETMEL
                     YRTSGTNPFSSCLPILAQSPFFFGLFRVLNGLQSIADGKREGIGPITREVASQAEQAM
                     IFGAQLSDKFMGASSANVKIVTVVLIILMSVTTFTTQRQLMTKNMPASALDNPFARQQ
                     KILLYVLPLVFAISGVNFPIGVLIYWFVTNVWSMGQQMYVIRRMPTPGSLAEKALHER
                     RARKGKPLPQGPVLGTAGGSTATGTVIDGPAHRQGGQRQQPKRKDRNRKPKSGPTQPG
                     NKPKPSDDDDPQDPPADQG"
     gene            10009..10716
                     /locus_tag="PKU75_10210"
     CDS             10009..10716
                     /locus_tag="PKU75_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HPF81295.1"
                     /translation="MADESQMEESALEEPTAEPVSDETVNAEPMSLEPVSQDPVSQDP
                     VSEERVGADDAASEDYGDGSDGEDGAPARGRGNRQAVLEREGEVAADFLETLLDIADL
                     DGDIDLDIDGDRAAVAIVDSDEGRVPRRLVGPNGKVLEALQELTRLAVQSATGERSRL
                     MLDIAGHRAERRAALVEQAKVAIAECRESGEPVDLDPMTAFERKVVHDEVLAAGLVSE
                     SSGVEPRRYVVVHPAAS"
     gene            10688..11419
                     /gene="rsmG"
                     /locus_tag="PKU75_10215"
     CDS             10688..11419
                     /gene="rsmG"
                     /locus_tag="PKU75_10215"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPF81296.1"
                     /translation="MSSSTPPPPDPAPGPPPAPAVAGEIFGEQLGPAGRFVAILADTG
                     VSHGLIGPREVPRLWERHVLGCAVVAEAMPREGASVIDVGSGAGLPGIALAIARPDLQ
                     VTLVEPMLRRTTWLTESVAALGLSNVTVCRGRAEEFHGQLVADYVTARAVAALDKLAA
                     WCAPLIAVGGELMVMKGDKASEELTAAAPVMARLGLETGTILTVGATLPQPTTLVVAR
                     KVRDGATGGMSRRSGKGSRPARHRT"
BASE COUNT         1729 a         3955 c         4051 g         1837 t
ORIGIN      
        1 gccgagcaca cccacctgcg cccacgcgga cttgacggcc gcgacctcgg gcgactgcgc
       61 gccatactgc ccgatcgcct cgtccaccgt cagcccggcg aaggtgacga aatcgcaatc
      121 ggcgctgatg ccggggccgg tgagcacggc ataccagatc cggcccggcg cctcccacga
      181 cgcgccgccc aaggcggtgg ccagcaggta gaaggcgcgg ttggggatgc cggagttgat
      241 gtgcaccccg ccgtggtcgt cgtcggtctc gacgtagtcg tccatgtgcc ccggctgcgg
      301 gtccttgccc aggagcgggc tgtcgtaggc ggtgcccggg gccttcatcg agcgcaacgc
      361 ctgccccgcg agttcgccga tgagcagttc ggcgccgatc aaccagtcgg cgtcggccgc
      421 accctgcccc agccgacgtt gcttgaccat ggacccgaag acgtcggaca tcgactcgtt
      481 gagcgcgccc ggctggcctt ggtacatcag cccggcggtg tgctcggtga ccccgtgcgc
      541 gagctcgtgc ccgatgacgt cgagacttgc ggtgaaccgg ccgaagatct tgccgtcacc
      601 gtcgccgaag accatctggg tgccgtccca gaaggcgttg tcgtagttct cgccgtagtg
      661 caccgtcgcg cgcagcggca gcccgcgccc gtcgagggag ttgcgctcga acgcgtcggc
      721 gaacaactgc cacgtgtcgc ccagcccgtc ataggcctcg gccaccgcga catcaccggt
      781 gtccggttcc ccctcgccgc gcacctgggt gcctgggagg gtcgtcccgc cctgggcatc
      841 acagatcagc cgttgcggcc caccctcgag agtccccggt gccaagcggg gtttgctgcc
      901 cggccggctc tcgggcgcgg cgtgggccct cctgtggagc atgccctcgg cgacatcgcg
      961 cagggcctgc cgagcggcgt cggcgatctc ggcgtcggcg gagtgggcca gccgctcgag
     1021 gatgtgcggc ggcacgatcg agcagcagta tgcggtcggc aagtgatccc ctgtcatgcc
     1081 cccatcctgt cggagcggtc cgacaaccgc gcgccgacgg gtcagccgta ggtgtcccgc
     1141 ggcccccggc cgccggtgat cccgcgccgc ccgtggctcc accgcggcgc cgtcgggccg
     1201 atgatctgca actccgtcac cgtgtccggc ccggcctcgg cggcgatccg cgccagcagg
     1261 ctggacgaga gcagccgcaa ctgggtggcc cacgacgtcg aagaggcccg cacgacgagc
     1321 acgccgtcgg cgaaactctc cgcgctcgcg tgcgccgcga tctccgcacc gacgatctgt
     1381 tcccaccggc cgacgacggc gccgacctcg acctcccgct cccacccgcg atcgctcacc
     1441 agccggtcga cgacgtcgcc gagcagcgcc gggtcacggt cggtgcgccc actctccggt
     1501 gcgccgccgc ggggggcgcg tttcggcata ctgccggggc gcagcccctt ggcgcgggcg
     1561 agggcacgcg ctcgcgccag tgcttcggcg ggggcgtcgg ggtccgacgg ggccaggttc
     1621 tcgtcactca tcgggagctt ccgggtatgc cgtgatctca cccttcgtca ccgagaacca
     1681 ttgtcccgca agggattttg gtatgtcgtc cgccaccgcc gccgtgatca gcacctgctc
     1741 gcagtccccg atgagcgccg ccagccgctc acgccggccg ctgtcgagtt cggcgaaaac
     1801 gtcgtcgagg atgagcaccg gatcggtgcc caggtcggtg cgcagcagtt ggtatgccgc
     1861 gagcttgagc cccagcgcga acgaccagga ctcgccgtgg ctggcatacc ccttggccgg
     1921 catctccccg agcgccagga cgaggtcgtc gcggtgcggg ccgacgaggc actgtccgcg
     1981 ctcgaactcg ttggcctgca cctcggcgag ggcggcgacg atgcctgcgg acaattcgtc
     2041 gatctcgggc acctcgccgg cggcgatgcg ggcgttcaac ccttgggtca gcgaggcccg
     2101 gtaggtcgct agcgcctccc cggccccgcc gctgacctcg cgataggcgt ggtcgaggtg
     2161 cgtgcgcaga tcgcgcagca gccgcaggcg ggcatacagc agttgggcgc cgagccccgc
     2221 gaggtggccg tcccagacgt gcagggtatg cagcacgctc tcgcgtgcct cctccgcgct
     2281 ggctccctcg gccggccgcc ggcctcggcc gccgcggcgc agcatcggcg cggactgttt
     2341 gagcagggcg ctgcgctgct tgacgatccg gtcgtagtcg gcgcgcaccg ccgcccagcg
     2401 cggctgccgt tgcacgagca gctcgtcgag aaaccggcgg cgcgcggccg ggtcgccctt
     2461 gacgagcgcg aggtcctcgg gcgcgaacag caccgatcgg acctgaccga gcacctcgcg
     2521 cggccgcggg accggcgagc ggttcagacg cgcccggttg gccttgccgg ggttgatctc
     2581 cagctcgacc agcgtctcgc ggccgtcgcg gaccaccccg ccgcgcacga tggcttggct
     2641 tgcgccgaag cgcaccagcg gttggtcggt ggagacccgg tgggaggaga gcgtcgccag
     2701 atagccaagt gcctcagcga tattcgtctt gccctggccg ttgaggccga ccagggtcgt
     2761 cacgcccggc ccgaggggga cctcggcagc cgggtagctg cggaagtcga tcaggctcag
     2821 gtggcggacg aacaccgggt cagcctaagg cgcgccggca tctcgccagc ggctggtcgg
     2881 agcagcaggc ctcgccaggc gtgctggttc gaccaacctc ggtcaggccc gcttggcggc
     2941 gcgtttgcgg gtgcttccgg tgcccgaggt cggaccggcg ccggcagcgc cggaaccgcc
     3001 ctgggtgctc ccgctgccgg aggacgggcc cgcggctgcg gcctttcggc ccttggccgc
     3061 ggcattggca gcgctcttgg tcggcttctc cttgggcacg gtccgcttct tgacggcgcg
     3121 cgggctcgcc ggcgcggccg cgccttcctc acgcagcttg tcgccctcgg cgaccgtcat
     3181 cacctggtgg ccaccgaact ggccgcgcag cgccgcgacg gcctgcatgg tcggggagtg
     3241 ctcctgccgg gaggcgaacc gggcgaagag ggaggcggag atgaccggca tgggcaccga
     3301 caaggcgatg gcctcctcga cggtccagcg gccctcaccg gagtcggcgg tgtagtccga
     3361 gacgttctcg agggtcgggt tctcctcgag cgccttgacc atcaggtcga gcagccagga
     3421 gcgcacgacg gtgccgcggc tccaggcctt gaaggcgccc ggcacattgg tgacgatgtc
     3481 cttcttctcg agcagctcgt agccctcggc ataggcgtgc atcaggccgt actcgatgcc
     3541 gttgtggacc atcttggtgt agtgcccggc gccgaccttg ccggcgtgca cgaaaccctc
     3601 gtcgcgcgga ccctcgggac gcagcgcatc gaagatcggc atggcgcgcc ggacgttggc
     3661 ggcggtgccg ccgcacatca ggccgtagcc gttctccagg ccccagatgc cgccggacac
     3721 gccacagtcc atgtagccga ttcccttggc cttcaacagc ttttcgttct cgaagtcgtc
     3781 ggtgaagcgg ctgttgccgc cgtcgatgac caggtcgcca ggctcgagca ggtcggccag
     3841 tttggcgacg gtggagcggg tgatctcgcc ggcggcgacc atcgtccaga ccacacgcgg
     3901 tgcctcgagc gacttgacga gttcggccaa ggacttgacg tccgagacct tggggttgat
     3961 gtcgtagccg atgacctcgt gacccgcgcg gcggatccgc tcgcgcatat tgccgcccat
     4021 cttgcccaag ccgatcaagc cgagttgcat gagtggcccc tctctggtcc gtgtccggta
     4081 tgcggtgccc gcgccccaca ctgtatcgcc gggcgctctc gcgtcgggtc gctggacgcc
     4141 gggtaacgcg ggccgggttt tcggtgtcgg gccaggtgtt cggtgccggg ccaggctctc
     4201 ggccactggg gccaccctgt cgttgtcggg cacggatata gccgtgcccc gcaacgaaaa
     4261 cctggcccga caaaccctca gctggtcagg cggaccggct gcaggacgta gcggtagctg
     4321 agttcgggtg agccgtccac ctcggcctgg ccggtgagca tggcgggctt gagggccgcg
     4381 gtgaaggaga agcgcgcgta gggggtatgc agcgcgccca gcccgtcgag caggaagccg
     4441 gggttgaagg cgatgtcgat gtcggcgccg tccagggtcg cctccacggc ctccgaggcc
     4501 tgcgcgtcgt caccggtgcc ggcctcgatg tcgacctgcc cgctggtgaa cttcagccgc
     4561 accggggtat tgcgttcggc gacgagggcc acgcgcttga ccgcctcgat cagatcggac
     4621 gtcttgacga cggcctcgat ctcggaagtg gcggggaaga tcttggcgac cttggggtat
     4681 tcgccatcga ggaggcggga ggtgctgtgc cgctgcccgg cctcgaaccc gacgagcccg
     4741 tcgccaccgg cggcctgccc gagcgcgacc gcgaccgagc cacccgaccc gagcgagcgg
     4801 gccgtctcgg agagcgtgcg ggccgggatg agagcgatgt gcgaggcgtc ggcggccgca
     4861 gggttccacg tgaactcacg catggccagg cggtagcggt cggtcgcgag gaaggtgacc
     4921 ttgtcaccct cgatctcgac gcgcacgccc gtgaggatgg gcagggtgtc gccgcggtcg
     4981 gcggcgacag cgacctggct gaccgcctgg gcgaaggtgg cgccgtcgat ggtgccgctg
     5041 gcttcgggct gggtcggcag ggtcggatag tccgaggccg gcatctgcag cagggcgaac
     5101 cgcgaggagc cgcacgtgac ctgcaccttg gagccgtccg tcgcgatgtc gaccggtttg
     5161 tcggcgggca ggttgcgggc gatctccgag aggagcttgc ccaggacgag gacggtgcct
     5221 ggctcggcga cctcggcgct gacggtgatc cgggccgaga cctcatagtc gaaggccgac
     5281 agggtcaccg tgccgtcggt gccggcatcg atgaggacac cggcgagcac cggcacggcc
     5341 ggacgcatcg gcagcccgcg ggccacccag gtgaccgcct cggcgagaac atcacgctcg
     5401 acacggaact tcactgttga ccaccttcgg cagttggatc gtggtgccgc ccatgcggcc
     5461 cgcccgaatc ggattgtcgg agcgcgcgac gacaaccgtc ggcgtccgat gggaaggatg
     5521 acattagccg cccgctcctt cttgtgccag ggcgggggcc gcttcgtgct cctgtcgtgt
     5581 cctgtcccca gctcgtggtc ggggccgaaa ccggtgagct agatgtattc gtcatcgtca
     5641 tagcgggtgt ggatcttgtg gacaagtcac catatgggca ggtcaacgcc ctgtttaccg
     5701 cgtggactgc gctgtggacg acgctccgcc gcagagcacc cgggtgtgga cgagtgcgcg
     5761 caatccccag gttggggagg attctgggga cggaaatggg tacctgacca caccgcatgt
     5821 gcatgctcgg tgctggtttt ccacaactgg tggtgggatc tcctgtatcg ccgtccacaa
     5881 agatgtccac atcttgtgga cgaacgcggg tgaattgccg gctcccgcac gaggaacagg
     5941 aggacagccg caggtgtcgg gaaggtaacg cgccatccac gggtgtggac aaacttgggg
     6001 aagacgggct gcggaagccg gcgtcagctc gcgctctgct gcttgatgcg gttggtcagc
     6061 tcggtgacct ggttgtagat cgcgcgacgc tcggccatca gctgccggat cttcttgtcc
     6121 gcgtgcatca ccgtcgtatg gtcgcggcca ccgaactgct gcccgatctt cggcagggac
     6181 aggtccgtca gttcgcggca caggtacatc gcgatctggc gggcggtgac cagcacccgc
     6241 gagcgggagg cgccttggag gtcgtcgatc gtcagcccga agtaggccgc cgtctgggcc
     6301 atgatcgtcg cgctggagat ctggttggtg tgctcgttgg ggatcaggtc cttcaggacg
     6361 atctcggcga gcggcaggtc gacggcctgg cggttgaggg aggcgaacgc ggtcacgcgg
     6421 atcagggcgc cctcgagctc gcggatattg gtcgagatcc gggaggcgat gtattcgagc
     6481 acctccggtg gcaccgagag ccgctcctgg atcgccttct tgcgcaggat cgcgatccgc
     6541 gtctcgaggt cgggcggctg gacatcggtg agcaggcccc actcgaaccg gctgaccatc
     6601 cggtcctcga agttgctcag gtgcttgggc tggacatcag aggtgatgac cacctgcttg
     6661 ttggcgttgt gcagggagtt gaaggtgtgg aagaactcct cctgggtctg caccttgccc
     6721 gagaggaact ggatgtcgtc gataaggagg acatcgacct cgcggtagcg ggactggaaa
     6781 ttcgccgccc ggtcgtcgcg gatggagttg atgaagtcat tggtgaactc ctcggagttc
     6841 acatagcgca cccgcaactg cggatagagg ctgcgggcgt agtggccgat cgcgtgcaac
     6901 aggtgggtct tgcccagccc ggatccgccg tagatgaaca aggggttgta ggccttggcc
     6961 ggtgcctctg cgaccgcgac cgccgcggca tgggcgaacc ggttgctcga cccgatgacg
     7021 aaggtgtcga agacgtattt cgggttcagc cgggtggcgc cgatgtcttc ggcgtccggc
     7081 agataggtga cgggcgcggc cgggagctcc tcgatctcct cgtcctcctc gaccacctcc
     7141 accggctcgg cgaggtcggc ggtggaactg aacaggtcgt cctcggtggc cagcgacggg
     7201 tcgacggtca ccgccagcgt cacctcgcca ccgagctcgc ggccaaggac cccgatcaca
     7261 tgctcccgga cgcgttgttc gaggatctgc ttcgcgaagt cgttggagac ggcgaccaga
     7321 acggtctgat cgagcacacc tttgaccttg gcgccttgca ggaaggcccg ctgattcatc
     7381 gggatcccgt cctgggcgag ggtggtcagc acgctgttcc acagcgctcc ggtgtccatc
     7441 gaagcttcgg ccacctgcaa catcccttca gccgggaacc ggcgtccaca tacttgtcca
     7501 cagcctgtgg gggacgcgga catcgctgcg aagcgagccc cgaacctaac aaggtgaacc
     7561 ggctgcggca accagagcag tatgacccgt cgcggccctg atcgcgaccg agagcgtgtc
     7621 ggtgatcgtc agtcgccggg cggcgtgtca ggatgcgagg ttggctggtg cgccggccac
     7681 ggtttgaccc gattccgcgc cctgccgtac cgttgatccc tggccatgtc ggccgttttc
     7741 gcatgcccac gccaccccgg cgcgggtttc cgattgcggc cgaaacacga cccgtatgtc
     7801 gtgtcggccg cacctgatcc ccaacaccat cgctggtgcc gcccgccgcg gccggtccct
     7861 tccaggccgc gcccggcagg caccggcctt tccgtgaaag agaagacgtc gtgagcaagc
     7921 ggaccttcca gcccaacaac cgtcgccgcg cgaagaacca cggtttccgt gcgcgcatga
     7981 gcacccgggc cggccgcgcc atcctcgccg cgcgccgccg caagggccgc gccgagttgt
     8041 cggcctgacg caggcacccc gcggtgctgc cggcacagca ccggctgcgc gcgagtggtg
     8101 acttcgcggc ggtcctgcgt cgccgcggtc gcgtcagtgc tgccgctcgc gtgctcgtcg
     8161 tgcatgccaa cgccataccc ggccgggagg acctcccgag ccgggtcggc ttcgtcgtgg
     8221 gcaaatccgt cggcaacgcc gtcgtgcgcg gacgcaccaa gcgccgcctg cgcgaacact
     8281 gccgatcccg gctcggcctg ctgatgcccg gcaccgacta cgtcgtgcgc gcccaacccg
     8341 ccgcggccgg ggccaccttc gccgaactcg gtgccgccct cgacgaggcg ctggcgcggc
     8401 tcggaccccg gtggagccgg accgcatgag ccagtcatcc tcggacacga ggccctcgag
     8461 caccgggccc tcgaacaccg ggccctcgaa caccaggccc catgccagcc tggggcgctt
     8521 gctcgcgctg ccgctgatct ggccgatccg gttctatcag cgcttcatct ccccggccac
     8581 gccgccgacc tgtcgctact accccacctg ttcggggtat gccgtgaccg ccctcgaacg
     8641 gttcggtccc gtcaaagggt tctacctcgc cgcccgccgg ctgctgcgct gccacccctg
     8701 ggcaccgggc ggggtggacc acgtacccga gacgttcacg tggcggcgag cgagcggcat
     8761 accggaagct ccgccgcagg cggaccccca cgagcacgga acttttccgg tcaccggcac
     8821 ttttgacacc actgcgccgc tcccggcgca ggacacccca cccaaggcga gctgaaggac
     8881 accatgggcg accttttcaa ttccatcctc tatcccattg agtggatcgt ggcgtggatc
     8941 atgtacggct tccaccaggc gttcaccgcc atcgggatgc cggcggcttc cggctggacg
     9001 tgggcgttgt ccatcgtcgg cctggtcatc gtcatgcggg cattgatgat cccgctgttt
     9061 gtcaagcaga tccacgcatc gcgcaagatg cagatgatca gccccgagct gcagaagatc
     9121 caggcgaaat acaagggcaa gaccgacccg gaatcgcgtc agaaaatgac gcaggaaacg
     9181 atggagctct atcggacctc ggggaccaat ccgttctcgt cgtgtttgcc gatcctggct
     9241 cagtcgccat tcttcttcgg tctgttccgg gtgctcaacg ggctgcagtc gattgccgac
     9301 ggcaagcgcg aaggcatcgg cccgatcacg cgcgaggtcg ccagccaggc cgaacaggcg
     9361 atgattttcg gggcgcagct gtcggacaag ttcatgggtg cctccagcgc gaacgtcaag
     9421 atcgtcacgg ttgtgctgat cattttgatg tcggtcacga cattcaccac gcagcgccag
     9481 ctgatgacca agaacatgcc ggcttcggca ctggacaacc ccttcgcgcg ccagcagaaa
     9541 atcttgctct atgtgctgcc gctggtattc gcgatctccg gcgtgaactt ccccatcggt
     9601 gtgctcatct actggttcgt caccaacgtg tggtcgatgg gccagcagat gtacgtcatc
     9661 cgccggatgc cgacccccgg ctcgctcgcc gagaaggccc tccacgagcg gcgcgcccgc
     9721 aagggcaagc ccctgccgca gggacccgtg ctcggcacgg cgggcgggtc cacggcgacc
     9781 ggcaccgtca tcgacgggcc cgcccacaga caaggcggcc agcgccagca acccaagcgc
     9841 aaggaccgca accgcaaacc caagtcggga ccgacgcagc cgggcaacaa gcccaaaccg
     9901 tcggacgacg acgatcccca ggatcccccc gcggaccagg gctaaggagt gacagtgagc
     9961 gagcaggaca acgccgtggt cgacgagacc gcggttcagg acaacgccat ggctgacgag
    10021 agccagatgg aggagtcggc tctcgaggag ccgaccgcgg agccggtgag tgacgagaca
    10081 gtgaatgcgg aaccgatgag tctggagccg gtgagtcagg atccggtgag tcaagatccg
    10141 gtgagtgagg agcgggtcgg cgctgacgac gctgcgagcg aggactacgg cgacggctcg
    10201 gacggggagg acggggcgcc ggcccggggc cggggcaacc ggcaggccgt gctggagcgg
    10261 gaaggcgagg tcgcggccga cttcctcgag acgctgctcg acatcgccga cctcgacggc
    10321 gacatcgacc tggacatcga cggcgatcgg gccgcggtgg cgatcgtcga ctccgacgag
    10381 ggccgggtgc cgcggcggct cgtcggaccg aacggcaagg tcctcgaggc gctgcaggag
    10441 ctgacccggc tcgccgtcca gtcggcgacg ggcgagcgct cgcgtctgat gctcgacatc
    10501 gccggccacc gggccgagcg ccgcgccgcc ctggtcgagc aggccaaggt cgccatcgcc
    10561 gagtgccgcg agagcggcga gccggtcgac ctggacccga tgaccgcctt cgaacgcaag
    10621 gtcgtgcacg acgaggtcct cgccgcgggc ctggtgtccg agtccagcgg ggtcgaaccc
    10681 cggcggtatg tcgtcgtcca ccccgccgcc tcctgacccc gcgccggggc cgccgccggc
    10741 accggccgtc gccggggaga tcttcggcga gcagctgggg ccggccgggc ggttcgtggc
    10801 gatcctggcc gacacgggtg tctcgcacgg gctgatcggt ccgcgcgagg tcccgcggtt
    10861 gtgggagcgc cacgtgctcg gctgcgccgt cgtggccgag gccatgcctc gggagggcgc
    10921 gtcggtgatc gacgtcgggt ccggggccgg cttgccgggc atcgcactcg ccatcgcccg
    10981 ccccgatctg caggtcacct tggtggagcc gatgttgcgg cggacgacgt ggctcaccga
    11041 atccgttgcc gcacttggtc tttcgaatgt cacggtatgc cgcggccgcg ccgaggagtt
    11101 ccacggccag ttggtggcgg actacgtcac cgcccgcgcc gtcgccgccc tcgacaagct
    11161 cgccgcctgg tgtgcgccgc tgatcgcggt cggcggcgaa ctcatggtga tgaagggcga
    11221 caaggcctcg gaggaactga cggccgcagc gccggtgatg gcgcgcctgg ggctcgagac
    11281 cggcacgatc ctcaccgtcg gggccaccct gccccagccc accacgctgg tggtcgcccg
    11341 caaggtccgc gatggcgcga ctggcggcat gtcacggcgc tccggcaagg gctcccgccc
    11401 ggcccgacat cggacgtagc cgtcccggcc cggctttacg ggccgtgcgc tacaagaacc
    11461 gcgagtatcg agttctcgtc cgggctgctg tgctggtcac gcgctcgccg gtggggtgcc
    11521 tgccgacttc gtgtcgtcgg cggcccgctt gagcagtgac ccagggctgg ag
//
LOCUS       DAOPJG010000103        11529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5879_length_11529_cov_7.510023, whole genome shotgun sequence.
ACCESSION   DAOPJG010000103 DAOPJG010000000
VERSION     DAOPJG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5879_length_11529_cov_7.510023"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKU75_10220"
     CDS             complement(<1..274)
                     /locus_tag="PKU75_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81297.1"
                     /translation="MTVEAGSSHEDLETTWQRQMVFWHLVFAAVWLGAVIVTLLDDSG
                     PRGRGLELACIVGVGVAYAALGARGLAREDEWQGAAYLLIAWGLVIT"
     gene            complement(307..1104)
                     /locus_tag="PKU75_10225"
     CDS             complement(307..1104)
                     /locus_tag="PKU75_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF81298.1"
                     /translation="MNTLRLAWDRTILELKMYARERDMVIFSFLFPVIMLAIFGVVFS
                     SEIDAGEKAQGMNAARVFLPGMIAAGVLLTSFQTLAMSVAIERDDGTLKRLRGTPLPP
                     VAYFLGKVGLVLITALAQLALLLVVARFVFDVRMPTEPAAWLHFAAFFVVAVVGGTTL
                     GVAYSSLANPRSVTAVVVGPLLVLQFISGVYFTFGALPSWLQQIAAVFPLKWIAQGMR
                     SVFYPASWAGQEMTGTWEVGRTLLVLAVWAVSGLVLCLRTFRWTRDS"
     gene            complement(1101..1970)
                     /locus_tag="PKU75_10230"
     CDS             complement(1101..1970)
                     /locus_tag="PKU75_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81299.1"
                     /translation="MTTPIQVTDLHKSYGTVAAVDGLGFEVAQGETVAILGPNGAGKT
                     TTVEILEGFRRADSGEVRVLGVDPATADRAWRDRIGIVSQSCTGLDLLTPREIIASTA
                     LAYRNPLDVDHVIEVTGLATKANARIRGLSGGQRRRVDMALGIVGRPELLFLDEPTTG
                     FDPQARRDFWSLIRALGEDGTTIVLTTHYLDEAEALASRVGIIAAGRMVALGTPSELG
                     GSGRTSTLAWDEGGRRATLHTDRPTAEIARLASRFGGEVPGLTVSRRSLEETYLALIG
                     PQSAVGNADEVAA"
     gene            complement(2069..3328)
                     /locus_tag="PKU75_10235"
     CDS             complement(2069..3328)
                     /locus_tag="PKU75_10235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPF81300.1"
                     /translation="MSEPTATEHPYYHPRPGTAWEGVDAAGSLVVGSAPLVTLANWQE
                     VPHHVWSFQHMRELMPSQVIEGTDEPRELLEAQRDLDGVTLSTPWAPTLPELLGRTDT
                     DAFLVLHGGRIIDERYFGDMTRRRRHLVMSVTKSVVSCVAGAITARGLLDPEAPLETY
                     VPELASCGYAGARVRTVLDMRSGVAFREGYLDPDSEVRVMERSMGWAPRNHGDPLGMY
                     PYILSTVSDRPHDGVFDYRSIETDVLGWIVERAAGERMSDLIGEHVWRPMGAFHDAEI
                     TADPLGVAIHDGGLSATLRDLGRFGQLLAEYGTVDGRQVIPASWITTAAEPPADVRDA
                     FARSVNEPYLPGGWYRNQFWFVPGDNGPILLCLGIHGQMVFVEWSTGLVGVKLSSWPL
                     PQDSEKLIATIEAFRAIGRFVHVAPAA"
     gene            3437..4681
                     /locus_tag="PKU75_10240"
     CDS             3437..4681
                     /locus_tag="PKU75_10240"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPF81301.1"
                     /translation="MTTLTTIRPPAADADWQAWMAYGLALAAASPPHHAARPAPAWAP
                     ARATAGRTELALTLHAYREDRPGPRWQQLARATWPAYQRWFRSEGTAARPSYDDCRTA
                     LRHHLPELVPTWERLTQLAMDATGASRDEAGRLLSLWCPTPFVTGCSQVLVPGPDPAL
                     IRSYDYDPALFEGVIATTDYSGRRRVLGTSDCLWGLLDGINDAGLAVSLTYGGRPGRG
                     TGSVGPGFGIPLVIRYLLETCADVPAAVAVLRRVPVAQAYNIGLVDTAGRHATVFVAP
                     GEDAEVSTQRIACNHRLDVVEHPHLAARLRSPERHAELTRLLARGTDEAGLSDAFLRP
                     PLRSREYGAGFGSLYAVALRPGAGTVEYRWPGRTWTRRFDDPDEVITVEVPHEPTGPG
                     FGERHACRGRGRHPRPRHDADS"
     gene            4977..6371
                     /locus_tag="PKU75_10245"
     CDS             4977..6371
                     /locus_tag="PKU75_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPF81302.1"
                     /translation="MTATTDEVRTDGLHKGLRQRHLTMIAIGGVIGAGLFVGSGVVIN
                     EAGPSAFITYALCGVLIIMVMQMLGEMAVAKPHTGSFSQYARDALGNWAGFSVAWLYW
                     YFWVIIVGFEAVAGAKILQYWIDLPLWVMSLVLMLLMTATNLYSVKTFGEFEYWFAGI
                     KVATILIFLVTVTLFVLGLWPGAQLDFSNLTAHGGFFPNGKTIILAGMVTAIFSMVGA
                     EIATIAGAESDDPERAVTKATRSVVWRISLFFVGSVFLLAVILPWNSTELGASPYVSA
                     FEKLGVPYAANLMNAVVLTSVLSCLNSGLYTASRMLFVLAREGEAPQRVTRINSRGVP
                     IYAILLSTIVGFLCVVAAYLSPKGIFSFLINSSGAVILFAYILIAASQLVLRRRAEAE
                     GRELKFKMWGFPYLTILTIVAMLAILVSMAWRADTRSQITLGLVSWGALMVAYVIIRA
                     RKKAPLTSDAPATS"
     gene            6368..7624
                     /locus_tag="PKU75_10250"
     CDS             6368..7624
                     /locus_tag="PKU75_10250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPF81303.1"
                     /translation="MTGLATIHPLSPGAHERGVTLDNWAEPPQNRWTFLHLDEVLATV
                     RIPASSRPEPLAVDMAPLTDLVSPRYEPTGRIVGHAPIWKVLAETFTDAFLVLHHGRL
                     KTESYAESMTASTRHLLMSVTKTVVGVIANALVDEGLLGFHDRVGDVIPQLRQSGFAG
                     ATVRDILDMRSGVRFSETYHDPDADVYRMEHAIHWRTPRADVPTTLHDFLATLPQERE
                     HGGTFHYRSSETNVLAWLCERAAGTDLATLIHEMVWEPMGAEDEAAITVDSVGTAVGD
                     GGMCATARDVARFAEMLRHSGISPSGRKVGPSWFTTDTLLGTEDLRTAFAETSSPTGM
                     PGGHYRNQVWVPFPDRTTFLALGIHGQMVYVNQRAAVTGVKLSTWPTARNAAYFYDSL
                     AAFEIVAAHLGKTHFAAHPAAIREHR"
     gene            7621..9081
                     /locus_tag="PKU75_10255"
     CDS             7621..9081
                     /locus_tag="PKU75_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HPF81304.1"
                     /translation="MRPTHELRNISEIRTFYRTNDEPIYFVGPTPFNLLGLDRWVRNF
                     NYVTYYDPWDGEHPRIITPAKVPDTTFTSSEQITNHLLTSDEVRTKLAARGGVPRVMA
                     VFIDEETEAICADLGYQLTLPPVALRKRLDSKLETTRLGNEAGAPSVPNTLAAADSYE
                     QLLRLAADAGLGEDLVIQLPYGDSGKTTFFIAGQEDWDRHAKDLAGKEAKIMKRIRNT
                     ALAVEACITRHGTIVGPFMIDLTGYDTMTPYKGGWCGNDVFPGVMSAKQRTAATSLVR
                     RLGDRLALEGYTGFFEVDTLIDLDTDEVYLGELNPRISGASPITMVTAGAYADLPLYL
                     FHLLEYMNVDYTIDVDEINERWSELAAIDQWSQLIIKQPEDEVSQILAAPRTGTWGIE
                     PGGGLAYRRPSRDWHGLEDESELFFMRVYGAGDYLFKGADLGIVMAKCRMQTDDPKSL
                     TPRCEQIVAGLRSQYVSASLSEPALPMPSLLGGKAL"
     gene            complement(9017..10636)
                     /locus_tag="PKU75_10260"
     CDS             complement(9017..10636)
                     /locus_tag="PKU75_10260"
                     /inference="COORDINATES: protein motif:HMM:NF019520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HPF81305.1"
                     /translation="MPLTIEQLCSQRDLGLRLVAGEQAKTNVIGWAHTSELPDPTRWL
                     EGGELLMTLGLQLPDDPVEQRLYVERLAAVGLAGLAFDAGVIHHAVPEAIVRAGDEFG
                     LPIVAIPGPTPFLAISRAVITSITADQVAEVAQLSRNQERLAGAALVSGSSGVLSSLQ
                     RTLSATAITLDASGELVEAAGPKSKALAERVGKHLSEGGRRGASRQRSFSHVDDEGYL
                     IVQKLGKVKGAGVLAVGRRRQLQPNERLLVAHAATLLALLLDRPAHLREAEMRLRRML
                     FRSLLRPGGEADLGLLRYVGFTPEQRVAVVAFVDTGAPRAAEALVDSALSATGVPVLI
                     MANSKGLAVLAPAQSIAEVAEVARAALERERAQPLSGGVGGSVDVSAVLDSFRQASAA
                     ARYAASHPGEVVDFSDLGIFDLLLGTQSRDTLAAIAGSVLSPLDAAVHGHPGDLLRTT
                     NEFVSHGGQWEATSAALGVHRHTLRSRIDRISALLGRDLTSASERAEVWLALKARELL
                     SLTSAGAADKAKGPSLPTGKASAKPARSKKR"
     gene            10809..>11529
                     /locus_tag="PKU75_10265"
     CDS             10809..>11529
                     /locus_tag="PKU75_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF81306.1"
                     /translation="MAPAPGIPVQEGWPEQTRIVHTAIPGPRSLARHAHRQQVLSTGL
                     GTVLPVFVERAGGGILVDVDGNHLIDFASGIAVTTVGSSAPAVVRRVRDQVAQVTHTC
                     FLVTEYDGYVEVGEKLNALTPGTHAKKSALFSTGAEAVENAVKIARVSTRRPAIVTFG
                     HAYHGRTLLTMTMTAKNAPYKDGFGPFAPEIYRAPLAYPYRWPTGPERCATEALAALE
                     DLVLKQIGANNTAAIVIEPIQG"
BASE COUNT         1895 a         4149 c         3737 g         1748 t
ORIGIN      
        1 cggtgatgac caggccccac gcaatgagca ggtaggccgc accctgccac tcgtcctcgc
       61 gagccagtcc gcgggcaccg agggcggcat acgccacgcc cacgccgacg atgcaggcca
      121 gctcgagtcc tcgaccccgc gggccggagt cgtccaagag ggtgacgatc accgcgccca
      181 gccacacggc cgcaaagacg agatgccaga agaccatctg ccgttgccag gtcgtctcca
      241 agtcctcgtg cgagcttccc gcctccacgg tcataccgtc acgctctcat ggccgaccct
      301 gccggcctac gaatcgcgcg tccagcggaa ggtgcgcagg cacaggacca gcccggatac
      361 tgcccatacc gccagcacca gcagggtccg gccgacctcc caggtgcccg tcatctcctg
      421 gccggcccag ctcgcgggat agaagaccga gcgcatgccc tgcgcgatcc acttcagtgg
      481 gaagacggcg gcgatctgct gcagccacga cggcaaggcg ccgaaggtga agtagacccc
      541 cgagatgaac tgcagcacca gcagcggccc cacgacgacc gccgtgaccg agcgcgggtt
      601 ggccagcgac gaataggcga cgccgagcgt cgtgccgccg acgacggcca ccacgaagaa
      661 ggcggcgaag tgcagccagg ccgccggctc cgtgggcatc cgcacgtcga agacgaaccg
      721 cgcgacaacg aggagcaggg ccagctgggc cagcgcggtg atcaggacca gcccgacctt
      781 gccgaggaaa tacgcgacag gtggtagcgg agtcccgcgc agtcgcttaa gcgtgccatc
      841 gtcgcgttcg atcgcgaccg acatcgcgag ggtctggaag ctggtgagca agacgcctgc
      901 ggcgatcatg cccggcagga agacccgcgc cgcgttcatg ccctgcgcct tctcgccggc
      961 gtcgatctcg gagctgaaga cgacgccgaa gatcgccagc atgatgaccg ggaagaggaa
     1021 cgagaagatg accatatccc gttctcgcgc atacattttc aactccagga tcgtgcgatc
     1081 ccaggcgagt ctcaacgtgt tcatgccgcc acctcatcgg cgttgccgac cgcggactgc
     1141 gggccgatga gcgcgagata cgtctcctcg agcgagcggc gggacaccgt caagccgggg
     1201 acctcgccgc cgaatcggct tgccagccgg gcgatctcgg ccgtcgggcg gtccgtatgc
     1261 agcgtggccc ggcgcccacc ctcatcccag gcgagcgtcg acgtccgccc cgacccgccg
     1321 agttcgctcg gagtcccgag cgccaccatg cggccggccg cgatgatgcc gacgcgggac
     1381 gccagcgcct cggcctcgtc cagatagtgc gtcgtcagca cgatggtcgt accgtcctcg
     1441 ccgagggctc ggatgaggga ccagaagtcg cggcgcgcct gcgggtcgaa tccggtggtc
     1501 ggttcgtcga ggaagagcag ttccggccgg ccgacgatcc ccagggccat gtcgactcgt
     1561 cgtctctggc cgccgctgag accgcggatc cgggcgttcg ccttggtcgc caggccggtc
     1621 acctcgatga cgtgatcgac gtcgagcgga ttgcggtatg ccagtgcggt gctcgcgatg
     1681 atctcgcgtg gggtgagcag gtcgagtccg gtgcagctct gggagacgat gccgatgcgg
     1741 tcgcgccatg cgcgatccgc agtggccggg tcgacgccga ggacgcggac ctctccgctg
     1801 tcggctcgtc ggaagccttc gaggatctcg acggtggtgg tcttgccggc gccgttcggg
     1861 ccgaggatcg cgacggtctc cccctgcgcg acctcgaacc cgagcccgtc gacggcggcc
     1921 accgtgccat aggacttgtg caggtcggtg acctggatcg gtgtcgtcat gtctcaacga
     1981 tgcgccgccg cggcgtggca ccaccacccg cgaccggttg acccaccggt ccaccggccg
     2041 gtggacgccg ctgccggggt ccaggtcctc aggcagcggg cgcgacgtga acgaagcgcc
     2101 cgatggcgcg gaaggcctcg atggtcgcga tcagcttctc gctgtcctgg ggcagcggcc
     2161 agctcgagag cttgacgccg accaggccgg tggaccactc gacgaagacc atctgtccgt
     2221 gaatgccgag gcagagcagg atcgggccgt tgtcgccggg gacgaaccag aactggttgc
     2281 gataccagcc acccgggaga tacggctcgt tgaccgagcg agcgaaggcg tcacgcacgt
     2341 cggccggcgg ctcggcggcc gtggtgatcc aggaggccgg gatcacctgc ctcccgtcga
     2401 ccgtgccgta ttcggccaag agctggccga aacgcccgag gtcgcgcaag gtcgccgaca
     2461 gacccccgtc gtggatggcg accccgagcg ggtcggccgt gatctcggca tcgtggaagg
     2521 ctcccatggg gcgccagacg tgctccccga tgagatccga catgcgttct cccgcagccc
     2581 gttccacgat ccagcccaga acgtcggtct cgatggaccg gtaatcgaag accccgtcat
     2641 gcgggcggtc cgacaccgtc gagaggatgt aggggtacat gcccagcggg tcgccatggt
     2701 tgcgcggggc ccaccccatg gaacgctcca tgacgcggac ctctgagtcg gggtcgaggt
     2761 agccctcccg gaaggcgaca cccgatcgca tgtcgaggac ggtgcgcacg cgcgcgccgg
     2821 cgtagccgca tgaagcgagt tctggcacat aggtttccag cggagcctcg ggatccagca
     2881 agccccgcgc cgtgatcgct ccggccacgc aggacacgac cgacttcgtc accgacatga
     2941 ccaggtgacg ccggcgacgt gtcatgtcgc cgaaatacct ctcgtcgatg atccgcccgc
     3001 catgcaggac gagaaaggcg tcggtatccg tgcgccccaa cagttccggc agcgtgggag
     3061 cccacggggt actcaaagtg acgccgtcca gatcacgttg cgcctcgagc agctcacggg
     3121 gctcgtcggt tccctcgatg acctggctcg gcatcagctc ccgcatgtgt tggaaggacc
     3181 acacatgatg cgggacttcc tgccaattcg ccagcgtgac cagcggcgcg gacccgacga
     3241 cgagggatcc ggccgcgtcc acaccctccc aggccgtgcc gggccgaggg tggtagtagg
     3301 ggtgttcggt cgccgtcggc tcgctcactc tcccatcgta ggaacccggc attgcgagcc
     3361 cgcgccgggc ccccgagacc gcgtcccgtc aaagccacct tgacacggcg cctttcgtca
     3421 aagcagactt gacgtcatga caaccttgac gacgatccgt ccccccgccg ccgacgccga
     3481 ctggcaagcc tggatggcct acggcctggc gctcgccgcg gcctcgccac cccaccacgc
     3541 cgcccgtccc gcgcccgcct gggccccggc acgtgccacg gcaggccgga ccgaactggc
     3601 cctcaccctg cacgcctacc gcgaggaccg gccgggtccg cgctggcaac aactggcccg
     3661 ggccacctgg ccggcatacc agcgctggtt ccgctcggaa gggacggccg cccgcccgtc
     3721 atacgacgac tgccgaaccg cactccggca ccacctgccc gaactggtgc ccacgtggga
     3781 acggctcacc caactcgcga tggacgcgac gggcgccagt cgcgacgaag ccggccggct
     3841 gctcagcctc tggtgcccga cccccttcgt cacgggctgt tcacaggtgc tcgtgcccgg
     3901 ccccgacccg gccctgatcc gcagctacga ctacgacccg gccctcttcg agggggtcat
     3961 cgccacgacc gactactccg gccggcgccg ggtgctcggc acctcggact gcctgtgggg
     4021 attgctcgac gggatcaacg acgccggcct ggccgtgtcc ttgacctacg gaggccggcc
     4081 ggggcgcggc acgggcagtg tcgggcccgg tttcggcata ccgctcgtca tccgctacct
     4141 gctcgagacg tgcgccgacg tccccgctgc ggtcgccgtg ctgcgccggg tcccggtcgc
     4201 gcaggcctac aacatcggcc tcgtcgacac ggccggacgg cacgcgaccg tcttcgtcgc
     4261 gccgggcgag gacgctgagg tcagcacgca gcggatcgcc tgcaaccacc gcctggacgt
     4321 cgtcgagcac ccgcacctgg cggcccggtt gcgcagcccg gagcgacacg ccgaactgac
     4381 ccggctgctg gcccgcggga ccgacgaggc ggggttgagc gacgctttcc tgcggccccc
     4441 gttgcgctcg cgagagtacg gtgcgggctt cgggagtctg tatgccgtgg ccctgcggcc
     4501 gggggcgggt actgtcgagt atcgctggcc ggggcgcacg tggacgcgcc ggttcgacga
     4561 tcccgacgag gtcatcaccg tggaggtgcc ccatgagccg accggccccg gattcggtga
     4621 gcgacacgcg tgccgcggcc gaggccgcca tccgcgccct cgccacgacg ccgactcctg
     4681 aggcgttcgc cgcgctgctg gagttgagtt ctctcaccgg ggtcgcgctg ggcgacagcg
     4741 cccgcctcct tgccgcgaca tcctcgtgga gtacggtggg ggaggcctcc ggaacgacga
     4801 agcaggcggc ctgggctcgc tggcacgggt gaccgctaat accccgaacg atgcgtgagt
     4861 agggccagat tgtccacacc acctcggccc acgggacgat ccggccctcg ctctgttcgc
     4921 tgagcgctga caacctccca ggaatcagag cctgcaaggt cggaggagtc agaagaatga
     4981 cagcgacaac ggacgaggtg cggaccgacg gtctgcacaa gggtctgcgg caacggcacc
     5041 tgacgatgat cgcgatcggc ggggtcatcg gagccggcct gttcgtcggc tccggggtcg
     5101 tgatcaacga ggccggcccc tccgccttta tcacctacgc cctgtgcggc gtcttgatca
     5161 tcatggtcat gcagatgctc ggggagatgg cagtggccaa gccacacacc ggatccttct
     5221 cgcaatacgc ccgggacgcc ctcggcaact gggcgggctt ttccgtcgcc tggctctatt
     5281 ggtacttctg ggtcatcatc gtcggcttcg aagccgtcgc cggcgcgaag atcctccagt
     5341 actggatcga tctcccgctc tgggtgatgt cgctcgtcct gatgctcttg atgacggcga
     5401 ccaacctcta ctccgtcaag acgttcggcg agttcgaata ctggttcgcc ggcatcaaag
     5461 tcgccaccat cttgatcttc ctcgtcaccg tgaccctgtt cgtcctcggg ttgtggccgg
     5521 gggcgcaact cgacttcagc aacctgacag cccacggcgg gttcttcccc aacggcaaga
     5581 cgatcatcct cgccggcatg gtgacggcca tcttctccat ggtgggggcg gagatcgcga
     5641 cgatcgccgg ggccgagtcc gacgaccccg agcgcgccgt gaccaaggcc acccgctccg
     5701 tcgtctggcg catctcgctc ttcttcgtcg gttcggtgtt cctcctcgcc gtgatcctgc
     5761 cgtggaattc caccgaactc ggcgcctcgc cctacgtcag cgcgttcgag aagctcggtg
     5821 tcccgtatgc cgcgaacctg atgaacgcgg tcgttctcac ctcggtgctc tcgtgcctga
     5881 actccgggct ctataccgca tcgcggatgc tcttcgtcct cgcacgcgag ggtgaggctc
     5941 cgcagcgggt gacccggatc aattcccggg gcgtgccgat ctacgcgatc ctgctctcga
     6001 cgatcgtcgg cttcctctgc gtcgtcgccg cctacctctc ccccaagggc atcttcagct
     6061 tcctcatcaa ctcctcgggc gctgtgatcc tcttcgccta catcctcatc gccgcgtcgc
     6121 agctcgtcct gcgccggcgg gcggaggccg agggccggga actgaagttc aagatgtggg
     6181 gcttccccta cctgaccatc ctcaccatcg tcgccatgct tgccatcctc gtctcgatgg
     6241 cctggcgggc ggacacccgc tcgcaaatca ctctcggcct tgtctcgtgg ggcgcgctca
     6301 tggttgccta cgtgatcatc cgggcccgca agaaggcgcc gctgacgtcc gacgcccccg
     6361 cgacgtcatg accgggttgg ccaccatcca cccgctctca cccggtgcgc acgagcgggg
     6421 ggtgaccctc gacaactggg ccgaaccgcc ccagaaccgg tggactttcc tgcacctcga
     6481 cgaggtcctg gcgacagtcc ggatccccgc gtcgtcgcgc cccgagccgt tggcggtgga
     6541 catggccccg ctcacggacc tcgtcagccc acgctacgag ccgaccgggc ggatcgtcgg
     6601 gcacgcgccg atctggaagg tgctggcgga gaccttcacc gacgccttcc tcgtcctcca
     6661 tcacgggcgg ctcaagaccg agtcgtatgc cgaatcgatg actgcctcga cccgccacct
     6721 cctgatgtcg gtcaccaaga ccgtcgtcgg cgtgatcgcc aacgccctgg tggacgaggg
     6781 gttgctcggg ttccacgacc gcgtcgggga cgtcatcccc caactgcggc aatcggggtt
     6841 cgccggggcc accgtgcgcg acatcctcga catgcgctcc ggtgtgcggt tcagcgagac
     6901 ctatcacgat cccgacgccg acgtctaccg gatggagcac gccatccatt ggcgcacccc
     6961 ccgggccgat gtcccgacca ccctgcacga cttcctcgcg accttgccgc aggagcggga
     7021 gcacggcggg accttccact accgctcgag cgagacgaac gtgctggcct ggctgtgcga
     7081 acgcgcggcc ggcaccgacc tggccaccct catccacgaa atggtgtggg agccgatggg
     7141 cgccgaggac gaggcagcca tcaccgtcga cagcgtcggc accgcggtcg gcgacggcgg
     7201 catgtgtgcc accgcccgcg acgtcgcccg cttcgccgag atgttgcgac acagcggcat
     7261 cagcccctca ggacgcaagg tcggcccgag ctggttcacg acggacaccc tcttgggcac
     7321 cgaggacttg cggaccgcgt tcgcggagac ctcctccccg acggggatgc ccggcggcca
     7381 ctaccgcaac caggtgtggg tgcccttccc cgaccgcacc accttcctcg ccctggggat
     7441 ccacggacag atggtctacg tcaaccagcg cgccgcggtg accggcgtca agctgtccac
     7501 gtggccgacc gcgcggaacg cggcctactt ctacgacagc ctcgcggcct tcgagatcgt
     7561 cgccgcccac ctcggcaaga cccacttcgc cgcccatcca gccgcaatcc gggagcatcg
     7621 atgagaccca cgcacgaact gcggaatatc tccgagatcc gcaccttcta ccggaccaat
     7681 gacgagccta tctacttcgt cgggccgaca ccgttcaacc ttctcgggct ggaccggtgg
     7741 gtgcgcaact tcaactacgt cacctactac gacccgtggg acggggagca cccgcggatc
     7801 atcacgccgg ccaaggtccc cgacacgacg ttcacctcct cggagcagat caccaaccac
     7861 ctgctgacct ccgacgaggt ccgcacgaaa ctcgcggcac gcgggggcgt cccgcgcgtc
     7921 atggcggtct tcatcgacga ggagaccgag gccatctgcg ccgacctggg ctaccaactc
     7981 accttgcccc cggtcgccct gcgcaaacgt ctcgactcca agctcgagac cacccggctc
     8041 ggcaacgagg ccggcgctcc cagcgtcccg aacacgctcg ccgccgccga cagttacgag
     8101 cagctgctca ggctcgccgc cgacgccggg ctcggtgagg acctggtcat ccaactcccc
     8161 tacggcgact ccgggaagac gacgttcttc attgccggac aggaggattg ggacaggcac
     8221 gccaaggatc tcgccggcaa ggaggcgaag atcatgaagc gcatccgcaa caccgcgctc
     8281 gcggtggagg cctgcatcac ccggcacggc accatcgtcg gcccgttcat gatcgacctc
     8341 accggatacg acacgatgac gccctacaag ggtgggtggt gcggcaacga cgtcttcccc
     8401 ggggtgatga gcgccaagca gcgcaccgcc gcgaccagcc tggtccgccg gctcggggac
     8461 cggctcgctc tcgagggcta caccggcttc ttcgaggtgg ataccctcat cgacctcgac
     8521 accgacgagg tctacctcgg cgaactcaac ccgcgcatct ccggggcctc accgatcacg
     8581 atggtcaccg ccggtgccta tgccgacctg cccctctatc tcttccacct gctcgaatac
     8641 atgaacgtcg actacacgat cgacgtcgac gagatcaacg agcgctggag cgaactggcc
     8701 gccatcgacc agtggagcca gctgatcatc aaacaacccg aggacgaggt cagccagatc
     8761 ctggcagccc cccgcaccgg gacgtggggg atcgagccgg gcggcgggct cgcctaccgg
     8821 cgcccgtcgc gggactggca cggattggag gacgagagcg agctgttctt catgcgggtc
     8881 tacggcgccg gcgactacct gttcaaggga gccgatctcg ggatcgtcat ggccaagtgc
     8941 cggatgcaga ccgatgatcc caagagcctc acgccccgct gcgagcagat cgtcgccggc
     9001 ctgcgatcgc agtacgtcag cgcttctttg agcgagccgg ctttgccgat gccttccctg
     9061 ttgggaggga aggcccttta gccttgtccg ccgcgccggc cgaggtgaga ctcaacagtt
     9121 cccttgcctt cagggcgagc caaacctccg cgcgttcgga ggcggaggtg agatcgcgcc
     9181 cgagaagtgc cgagatccgg tcgatgcggc tgcggagggt atgccggtgc accccgaggg
     9241 cggccgaggt cgcctcccac tgaccgccgt gggagacgaa ctcgttggtg gtgcgcaaca
     9301 ggtctcccgg atgtccgtga accgcggcat ccagcgggga gagcaccgat ccggcgatgg
     9361 ccgcgagcgt gtcgcgggac tgggtgccga gcaagaggtc gaagattccg aggtcggaga
     9421 agtcgacgac ctcgccgggg tgtgaggcgg cataccgtgc cgcggcgctg gcctgccgga
     9481 agctgtccag caccgcggac acgtcgaccg acccgccgac gcccccggac aacggttgcg
     9541 cacgttcccg ttccagtgcc gcgcgagcca cctcggcgac ctccgcgatg gactgcgccg
     9601 gcgccagcac ggcgaggccc ttggagttcg ccatgatcag gacgggcacg ccggtggcgc
     9661 tcaacgcact gtcgacgagc gcctcggcgg cccgcggagc gccggtatcg acgaaggcga
     9721 cgaccgccac gcgctgctcc ggcgtgaacc cgacataccg caacaggccc aggtccgcct
     9781 caccgccggg ccgcaagagg gaccggaaca gcatccgccg cagccgcatc tccgcctcgc
     9841 gcaggtgcgc cggacggtcg aggagaaggg ccagcaaggt ggcagcatgc gcgacgagta
     9901 gccgctcgtt gggctgcaac tggcgccgcc ggccgacggc gaggactccg gctcctttga
     9961 ccttgccgag cttctgcacg atcaggtagc cctcgtcgtc cacgtgactg aagctgcgtt
    10021 ggcgcgatgc gccgcgacga ccgccctcag acaaatgctt gcccacgcgc tcggccaggg
    10081 ccttgctctt cgggccggcc gcctcgacga gttcgccgga ggcgtccagg gtgatggccg
    10141 tcgcggacag cgtccgctgc aggctcgaca acaccccgct cgagccgctc accagcgcgg
    10201 cgcccgccag ccgctcctgg tttcggctca actgagccac ctcggcgacc tgatccgcgg
    10261 tgatcgaggt gatgaccgcc cgggagatcg cgaggaacgg ggtcggaccc gggatggcga
    10321 cgatcggaag accgaactcg tcacccgccc ggacgatcgc ctcgggcacc gcgtggtgaa
    10381 tgacgccggc gtcgaacgcg agtccggcca gaccgaccgc ggcgagccgc tcgacataca
    10441 gtcgttgctc gaccgggtcg tcgggcagct gcagcccgag cgtcatgagc agttccccgc
    10501 cctcgagcca tcgcgtcggg tcgggcaact cgctcgtgtg cgcccaaccg atgacgttcg
    10561 tcttggcctg ctcgccggcc accagacgca ggccgagatc tcgttgcgag cacaattgct
    10621 cgatcgtcaa tggcacggcg gtcacctcac cctcaagtgg acatctcgtc cggagtatgt
    10681 cggtgaacgg gactttgcgt ccagagacgc cgactcgcgc cgctgcctac cgtgacagga
    10741 agcgccgggc ccgacaggag ccctgcgacg acgctacgga ggctgacatg gtggcagata
    10801 tcgcaacgat ggcaccggca ccagggatcc cggtgcagga gggctggccc gagcagaccc
    10861 gcatcgtgca caccgcaatc cccgggccgc gatccctcgc ccggcatgcg caccggcagc
    10921 aggttctctc caccggtctc gggaccgtgt tgccggtctt cgtcgagcgc gccggcgggg
    10981 gcatcctcgt ggacgtcgac ggcaatcatc tgatcgactt cgcgtccggg atcgccgtga
    11041 cgacggttgg ttcgagtgcc ccggccgtcg tacgccgcgt gcgcgaccag gtcgcccagg
    11101 tcacccacac gtgtttcctc gtgaccgagt acgacggcta tgtcgaggtc ggcgagaagc
    11161 tcaacgccct cactccgggc acgcacgcga agaagtccgc gctcttctcg accggcgccg
    11221 aggcggtcga gaacgccgtc aagatcgctc gcgtctcaac ccgtcgcccg gccatcgtca
    11281 ccttcgggca cgcctaccac ggccggacgt tgttgacgat gacgatgacg gccaagaacg
    11341 ccccctacaa ggacggcttc ggccccttcg ccccggagat ctaccgcgcg ccgctcgcct
    11401 atccctaccg ctggccgacc ggaccggagc gctgcgccac cgaggccctg gccgccctgg
    11461 aggacctcgt cctgaagcag atcggcgcaa ataacaccgc tgccatcgtc atcgagccga
    11521 tccagggcg
//
LOCUS       DAOPJG010000104        11490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5904_length_11490_cov_7.826148, whole genome shotgun sequence.
ACCESSION   DAOPJG010000104 DAOPJG010000000
VERSION     DAOPJG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5904_length_11490_cov_7.826148"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /gene="rsgA"
                     /locus_tag="PKU75_10270"
     CDS             complement(<1..735)
                     /gene="rsgA"
                     /locus_tag="PKU75_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPF81307.1"
                     /translation="MSRRDWDEDDVRVRPNRRGSRPRSKDRPAHSDAEIALVTAVDRG
                     RYTTLLEDDGDKRVVLAMKARELGRKGIVVGDRVGLVGDTGGGPDALARIVRIQPRTS
                     VLRRTADDTDPVERAIVANVDQLVVVSALADPEPRPRLIDRCLVAAYDAGLDPLLVLT
                     KADLVDPAGFLANYAPLEVPCVVTSTEGSDFAGLESVREHLHDRVSVLVGHSGVGKST
                     LVNALVPQADRATGAVNDVTGRGRHTS"
     gene            complement(732..2051)
                     /gene="aroA"
                     /locus_tag="PKU75_10275"
     CDS             complement(732..2051)
                     /gene="aroA"
                     /locus_tag="PKU75_10275"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPF81308.1"
                     /translation="MPSADADAWAAPVAAGPVDAVVTLPGSKSLTNRYLVLAALAEEP
                     SRLRAPLRSRDTTLMAAALRALGCRVEDAPAQSADWIVTPGPLSGPASIDCGLAGNVM
                     RFVPPLAGLARGPVAFTGDAHAASRPMAPVLGALRALGVAIDDGGRGGLPFTVAGSGQ
                     VRGGEVTLDASASSQFVSGLLLSGARYDDGVTVHHDGKSVPSLPHIEMTVETLRDAGA
                     VVDDSEPHTWRVEPSEINALDVVVEPDLSNAAQFLAAALVTGGRVRIPGWPQYTTQGG
                     DALRDILDQMGAEVHLDRDGLTVAAGEAIHGIDVDLHDSSELTPVIAALCAVADGRSI
                     IRGVAHIRGHETDRIAALASELERVGCEVAQTADGLRITPRPLRAARWQTYADHRMVM
                     AGAVLGLVTPGLVVEDVGTVAKTLPTFVTLWDDMLRQGGTPRAGGGR"
     gene            complement(2038..2580)
                     /locus_tag="PKU75_10280"
     CDS             complement(2038..2580)
                     /locus_tag="PKU75_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPF81309.1"
                     /translation="MSLVRRIARPLLATIFITGGLDALRHPSSKAQAAEPMVKALAGP
                     LGLPDDTELLVRANGATMIAGGALLATGRMPRVAATALAGSLVPSTIAGHPFWNETDP
                     QKKAEQKQQFMKNLSVLGGVLLAAVDTAGKPGLAYRARMLGDAASRTKGSIVKDARYA
                     AGTARREAKLKLATATDALS"
     gene            2690..3517
                     /locus_tag="PKU75_10285"
     CDS             2690..3517
                     /locus_tag="PKU75_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPF81310.1"
                     /translation="MCGRYAASADLDELIEEFDVELDRHDEPARSVLANPQQPPAGLP
                     DWNMAPTKQAPVVLTRPVDDVPRRQLRLLTWGLVPAWSKDVKGAYRMINARAESVAEK
                     PAYARALASRRALVPALGWYEWQTSPTVRDAKGKPRKQPFFVRRGDGDLLAMAGIYEF
                     WRDKAVADPDDPLAWLVTFAVVTTAAEPGLDRIHDRQPLVLEREDWARWLDPSVTEVA
                     AIDDLVHFCAPGRFTAYPVSRAVSSNRANGPGLIEPAPAGELQGVVDPATGEILGGA"
     gene            complement(3570..4550)
                     /locus_tag="PKU75_10290"
     CDS             complement(3570..4550)
                     /locus_tag="PKU75_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HPF81311.1"
                     /translation="MTDSLGPATRVLAAAVARRHYMAGESKVDIAAALGISRFKVARL
                     LDLAHEEGIVRIEIASDDIVDLELSEQIRELWGLRNCAVVLGHGPDPAAREALAQTAA
                     DLLCEVITPTDVLGLPWARTVSDMIRAIRELPPVEVVQLSGSLVVPDETTSPVDIVGR
                     AARIAGSRARLFYAPLILDDAASAAVLRKQPSVKAALSAADRVTISAAGIGAWGPKVS
                     TIYDTITDEDRAQAAAAGVVGEVMGVYFTVDGSLATTPLADRMVTVAADTLRGIPEVI
                     GIARGRAKAPAVMGAVRAGLLKSLVIDTEIAQLLLSTDPAPARGTSASKR"
     gene            4678..6045
                     /locus_tag="PKU75_10295"
     CDS             4678..6045
                     /locus_tag="PKU75_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPF81312.1"
                     /translation="MAGRKFWAFTSATAVVTAGLAGCSGAGGGGGGGTGASGGGGAVN
                     VLMVNNPQMVDLQKLTADNFTKSTGIKVNYTVLPENDVRAKISQEFTAQAGQYDVASL
                     SNFEIPIYAKNGWVMDLTEKANGDSAFNQSDIMKSMATSLSVDGKIYGEPFYGESSFM
                     MYRKDIFDAKGLTMPDNPTWDEIADLAAKADGAESGMKGICLRGMPGWGQVFAPLTTV
                     VNTFGGTWFEKDWTAGVNAAPFKEAVNFYVDLVKAHGEAGAPQAGFTECLNNMTQGKV
                     AMWYDATSAAGSLEAEGSPVKGKIGYAAAPVKVTKSSGWLYTWAWAVQKASKNQDNAW
                     KFISWASSKEYEELVGKQLGWAKVPSGKRASTYSNADYLKAAGAFAEKEKAAIEAADP
                     LNPGAQPRPAMGIQFIGIPEFPKLGTDVSQLVSSAIAGQTTVDAALDEGQKLAEAVSA
                     KYKGK"
     gene            6137..6988
                     /locus_tag="PKU75_10300"
     CDS             6137..6988
                     /locus_tag="PKU75_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF81313.1"
                     /translation="MPLLPAMVLVFLVTQLPFLFTIGASFMNWNAYYPDERRFNGGAN
                     FKTVLTDPAARKAIWVTVLLTAAVVLISLFLGLIIALLLDRKFFGQGIVRTMMITPFL
                     VVPVAAALLWKHALYNPEYGLFNGMLTKLFGENAPQPDWISEHPMWAVIFAIVWQWTP
                     FMMLILLAGLQSRPMDVVEAAKIDGASSWQIFRSMTLPHLRPYMELSGLLGTIYVVQN
                     FDHVFTITGGGLGTANLPYYIYTTFYTGQDYGLASAAGVVVVILTIIVSTVALRTVFS
                     IFREEQR"
     gene            6985..7842
                     /locus_tag="PKU75_10305"
     CDS             6985..7842
                     /locus_tag="PKU75_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPF81314.1"
                     /translation="MSGKTLSTGQVENVHKKSTSGVLLSIAAWIVGLLFVSPVLWMIL
                     TSFHSETDAAKNPPAFGAPLTLDGYRSFFDAGPWPPLINSVMASVVSTLLVLLLAFPA
                     AYALSIRPVKKWSDVLFFLLSTKFLPVVAGLLPIYLFAKKVGMLDNIWLLILFYTVMN
                     LPIAIWMLRSFLAEVPVEMLEAAEVDGAGLVLTLRKVVAPVVMPGLASAALICFIFSW
                     NELLFARVLTGTVSQTAPVYLTGFVTSQGLFLAQVCAASLVVSLPVLVAGFAAQDKLV
                     QGLSMGAVR"
     gene            7842..9326
                     /locus_tag="PKU75_10310"
     CDS             7842..9326
                     /locus_tag="PKU75_10310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HPF81315.1"
                     /translation="MGTPIPLGNATLGELPSEVGVPAYDRSALTPGIVHFGVGGFHRA
                     HEAMYLDALLNEGKALDWGIVGVGTMPGDARMRDAMKAQDCLFTLVLKHPDGSRDARV
                     IGGMVDYLFAPDDPQAVLERLADPSTRIVSLTVTEGGYHVNQVTGEFDPNSPDLRADL
                     AGDAPPKSVFGFITEAARMRRERGIPPFTVMSCDNLPGNGHIAEKMITAFAELKDPEL
                     AEHLRQDVAFPNCMVDRITPVTQQADIDDLAERFGVADRWPVVCEPFTQWVLEDNFGA
                     GRPAFEEVGVQIVPDVVPYELMKLRLLNASHQALCYLGYLSGYRYAHEVCQDPLFVKF
                     LLGYMENEGSPTLPEVPGVDLDAYRHQLIERFANPEVRDTLARLCAESSDRIPKWLVP
                     VIQENLRNGRDIDRSALVVASWARYAEGVDEQGDPIDVVDRFKDKVMAAAAAQQDDDL
                     AFLRDKDFFGNLVDDERFTTAYRGFLASLHERGARATLEELEGR"
     gene            9422..10834
                     /gene="xylB"
                     /locus_tag="PKU75_10315"
     CDS             9422..10834
                     /gene="xylB"
                     /locus_tag="PKU75_10315"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HPF81316.1"
                     /translation="MTRSLVAGVDSSTQSCKIVVCDAETGQVVRSARAPHPDGTEVDP
                     AAWWAAWQQASSGGLLDGVEAIAVGGQQHGMVTLDDADQVVRPALLWNDTRSAGAAAD
                     LVEEFGGAQAWAEEIGIVPVAAITATKLRWLARNEPDNMARTRTCVLPHDWVTGQILK
                     DGNGFQQWVTDRGDASGTGYWSAHSQDYRTDVMDLAMGRTIEVPTVLAPHEAAGRTDS
                     GMIVAPGTGDNMGAALGLGLARGDVVVSLGTSGTVFARHTDPVADASGALQSFADAEG
                     GYLPLLCTLNASRVINAGMAMLGVDYSEFDRLAMAAAPGAGGLTLIPYLDGERTPNLP
                     DATGTLLGLQRANATPENLARAHVEGMLLNLVAGVSALRDHGVAVERILLIGGAAAST
                     AVQDIACQLFGLPLVVPAAGEYVGLGAARQAAWTLSGADAPPQWEVSLDVRAEPGAPD
                     AGAEIVERYRQAVRDQHGVG"
     gene            10854..11471
                     /locus_tag="PKU75_10320"
     CDS             10854..11471
                     /locus_tag="PKU75_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81317.1"
                     /translation="MAEQVTLKTPQGPGRAHVYSGSVPVRATVVLGHGAGGGIGASDV
                     MLLASRLPEHGYRVVLIEQPWKVAGRKVAGRPPTLDAAWIPMLGQLDVVGSLVVGGRS
                     AGARVACRTAETVGAQGVLCLSFPLHPPGKPDASRAGELRTPGEHGIPVRVIQGERDP
                     FGTPDELRAELADPAWVAAVAGTHSFGKKPADVLDAAIRLLPELL"
BASE COUNT         1749 a         3967 c         4022 g         1752 t
ORIGIN      
        1 ggaggtatgc cgcccacgcc cggtgacgtc gttgaccgcg cccgtcgccc ggtcggcctg
       61 tggcacgagc gcgttgacga gcgtcgattt gccgacgccc gagtgcccga cgagcacgct
      121 cacccggtcg tgcaggtgtt ctcgcacgct ctcgagcccg gcaaagtctg acccttcagt
      181 cgaggtcacg acacaaggga cctcgagcgg cgcgtagttg gccaggaatc cggccgggtc
      241 gaccaggtcc gccttggtca gcacgagcag cgggtccagc ccagcgtcgt atgccgcgac
      301 gaggcagcga tcgatcagcc gtggccgcgg ctccgggtcg gcgagcgccg agacgacgac
      361 gagttggtcg acattggcca cgatcgcccg ctccacgggg tcggtgtcgt cggcggtgcg
      421 ccgcaggacg ctggtgcgcg gttggatgcg cacgatgcgg gccagggcat cgggtccgcc
      481 gccggtgtcg ccgacgaggc cgacccggtc acccacgacg atgcccttgc ggcccagttc
      541 gcgggccttc atggccagga cgacccgctt gtcaccgtcg tcctcgagca gcgtggtgta
      601 acggccccgg tcgacggcgg tgaccagggc gatctcggcg tcgctgtgcg ccggacggtc
      661 cttggaccgg ggacgagagc cacgtcggtt ggggcggacc cggacatcgt cctcgtccca
      721 gtcgcgccgg ctcaccggcc gcccccggcc cgcggtgtgc caccctgccg cagcatgtcg
      781 tcccagagcg tgacgaaggt gggcagggtc ttggcgacgg tgccgacatc ctcgacgacg
      841 aggcccgggg tgaccagccc gaggacggcg ccggccatga ccatgcggtg atcggcatac
      901 gtctgccagc gggcggcgcg cagcgggcga ggggtgatcc gcaggccgtc cgcggtctgg
      961 gccacctcgc acccgacccg ctcgagttcg ctcgccagcg ccgcgatgcg gtcggtctcg
     1021 tgcccgcgga tgtgggcgac gccgcggatg atgctgcgcc cgtcggccac cgcgcacaac
     1081 gcggcgatca cgggggtcaa ctcgctggag tcgtgcaggt cgacgtcgat gccgtggatc
     1141 gcctcaccgg cggcgacggt cagcccgtcg cggtcgaggt ggacctccgc ccccatctgg
     1201 tcgaggatgt cgcgcagcgc gtctccgccc tgggtggtgt attgcggcca cccggggatc
     1261 cgcacccgcc cccccgtgac gagcgcggcc gcaaggaact gggcggcatt cgacagatcc
     1321 ggctcgacga ccacgtcgag cgcgttgatc tcactgggtt cgacgcgcca cgtgtgcggt
     1381 tcgctgtcgt cgacgacggc gccggcgtcg cggagggtct cgacggtcat ctcgatgtgc
     1441 ggcaggctgg ggacactctt gccgtcgtga tggacggtga ccccgtcgtc ataccgcgcc
     1501 ccggacaaca acaggccgga cacgaactgc gacgaggcgg aggcgtcgag ggtcacctcg
     1561 ccgccgcgca cctggccgct gcccgcgacg gtgaagggca gcccgccccg cccgccgtcg
     1621 tcgatggcga cgccgagggc ccgcagcgca ccgagcacgg gcgccatcgg gcggctggcc
     1681 gcgtgcgcat cgccggtgaa ggcgaccggg ccgcgcgcca gccccgccag cggcggcacg
     1741 aaacgcatga cattgcccgc cagcccgcaa tcgatgcttg ccggacccga cagcggcccg
     1801 ggcgtgacga tccagtccgc cgactgcgcg ggagcgtcct cgacgcgaca gccgagggcg
     1861 cggagcgccg cggccatcag ggtcgtgtcc cgcgagcgca gcggcgcgcg cagccgcgac
     1921 ggctcctccg cgagggccgc gaggacgaga tagcgattgg tcagggattt gctgccgggc
     1981 agggtgacca cggcatcgac gggtccggcc gcgaccggcg cggcccaagc atcagcgtca
     2041 gctgagggca tcggtggccg tggccagctt gagtttcgct tcgcggcgag ccgtgcccgc
     2101 ggcataccgg gcgtccttga cgatcgaccc cttggtgcgc gaggcggcat cgccgagcat
     2161 cctggcgcga taggccagcc ccggcttgcc cgccgtgtcg accgcggcga gcaggacgcc
     2221 gccgagcacg ctgaggttct tcatgaactg ctgcttctgc tcggccttct tctgcgggtc
     2281 ggtctcgttc cagaacgggt gaccggcgat cgtcgacggg acgagcgagc cggccagcgc
     2341 cgtggcggcg acccgcggca tccggcccgt ggccagcagg gcgccgccgg cgatcatcgt
     2401 cgcgccattg gcgcgcacga gcagttcggt gtcgtcgggc agccccagcg gcccggcgag
     2461 tgccttgacc atcggctcgg cggcctgcgc cttgctgctc gggtgacgca gtgcgtccag
     2521 gccaccggtg atgaagatcg tggcgagcag gggtcgcgcg atgcggcgca ccaggctcat
     2581 aggttcctcc ttggcagggt cgacgtcggt ctgccctcta ccgtagacgc cgaactccct
     2641 cgccggtgcc ctggcgggcg gagaacccga gcagagcagc ggaggagaca tgtgcggacg
     2701 gtatgccgcc agcgcagacc tggatgagtt gatcgaggag ttcgacgtcg agctggaccg
     2761 gcacgacgaa cccgcgcggt cggtgctggc gaacccgcag cagccgccgg cgggactgcc
     2821 ggactggaac atggcgccca ccaagcaggc accggtcgtt ctcacgcggc cggtcgacga
     2881 tgtgccccgg cgccagctgc gcctgctgac ctggggcctg gtgcccgcgt ggagcaagga
     2941 cgtcaagggt gcctaccgga tgatcaacgc gcgcgccgag agcgtcgccg agaagccggc
     3001 ctatgcgcgg gcgctggcct cccggcgcgc cctggtgccg gcgctcggct ggtatgagtg
     3061 gcagacctcg cctacggtga gggacgcgaa gggcaagccg cgcaagcagc ccttcttcgt
     3121 ccgccgtggc gacggcgacc tgctcgcgat ggctgggatc tacgagttct ggcgggacaa
     3181 ggcggtggcc gacccggacg acccgctggc ctggctggtc accttcgccg tcgtcaccac
     3241 ggccgccgag ccggggctgg accgcatcca cgaccggcag ccgctggtgc tcgagcggga
     3301 ggactgggcg cgctggctcg atccgtcggt caccgaggtc gcggcgatcg acgacctcgt
     3361 ccacttctgc gcacccggcc ggttcaccgc atacccggtc tcgcgcgcgg tcagttccaa
     3421 ccgggccaac gggccgggcc tgatcgaacc cgccccggcc ggcgaactgc agggcgtggt
     3481 cgaccccgcg accggggaga tcctcggtgg cgcctgatct cagcagaggt tagtcaaatc
     3541 agcagacctc cctaggtgtg ctgatttcac taacgtttgc tagcagaggt tcctcgggcg
     3601 ggggcggggt cggtggacag gaggagctgg gcgatctcgg tgtcgatgac gaggctcttg
     3661 agcagtcccg cccgcacggc gcccatgacg gccggcgcct tggcgcggcc gcgggcgatc
     3721 ccgatgacct ccggtatgcc gcgcagcgtg tccgccgcga cggtcaccat ccggtcggcc
     3781 aggggagtcg tggcgaggga gccgtcgaca gtgaagtaga cacccatgac ctcgccgacc
     3841 accccggccg ccgcggcctg ggcgcggtcc tcgtcggtga tggtgtcgta gatggtggag
     3901 actttcggtc cccacgcgcc gatgccggct gccgagatcg tcacccgatc cgcggcgctc
     3961 aaggctgcct tgacggaggg ttgcttgcgc aggaccgcgg cggaggcggc gtcgtcgagg
     4021 atgagggggg catagaagag ccgggcgcgg ctgccggcga tccgggcggc ccggccgacg
     4081 atgtcgaccg ggctggtcgt ctcgtcgggc acgacgaggg agccgctgag ctggacgacc
     4141 tcgaccgggg ggagttcgcg gatcgcgcgg atcatgtccg agaccgtgcg ggcccacggc
     4201 aggcccagga cgtcggtcgg ggtgatgacc tcgcagagca gatcggcggc ggtctgggcg
     4261 agtgcctcgc gggccgccgg gtccggcccg tggccgagga cgacggcgca gttgcgcagc
     4321 ccccagagct ctcgaatctg ttcggagagt tcgagatcca cgatgtcgtc gctcgcgatc
     4381 tcgatgcgga cgatgccctc ctcatgggcg aggtcgagca gccgggccac cttgaagcgg
     4441 ctgatcccca gggcggcggc gatgtcgacc ttagactcgc ccgccatgta gtgccgacgg
     4501 gcgaccgcgg cggcgagcac ccgggtcgcg ggtcctaacg aatcggtcac ggctcgatcc
     4561 catctggtga tgtcgtcctt gacaagcggt cctgcggccc actttactca tatgtgcgcg
     4621 gagtgcgctc aaatgagcgc gacaaacccg ggcgaacact caacgaggag agatcgcatg
     4681 gctggtagga agttctgggc attcacgagt gcgaccgcgg tcgtcaccgc gggtctggcc
     4741 ggatgctccg gcgccggtgg gggcggcggc ggcggcacgg gcgcgagcgg cggcggtggg
     4801 gccgtcaacg tcctcatggt caacaacccg cagatggttg acctgcaaaa gctcacggcc
     4861 gacaatttca ccaagagcac gggcatcaag gtcaactaca ccgtcctgcc ggagaacgac
     4921 gtccgcgcca agatctccca ggagttcacg gcacaggcgg gtcagtacga cgtcgcgtcg
     4981 ctgtccaact tcgagatccc catctatgcc aagaacggtt gggtcatgga cctgaccgag
     5041 aaggccaacg gcgacagcgc gttcaaccag tccgacatca tgaagtcgat ggcgacctcg
     5101 ctgtccgtcg acggcaagat ctacggcgag ccgttctacg gcgagtcgtc gttcatgatg
     5161 taccgcaagg acatcttcga cgccaagggt ctgaccatgc ccgacaaccc cacgtgggac
     5221 gagatcgccg acctcgccgc gaaggccgac ggcgccgagt cggggatgaa gggcatctgc
     5281 ctgcgcggta tgccgggctg gggccaggtc ttcgccccgc tcaccacggt cgtcaacacc
     5341 ttcggcggca cctggttcga gaaggactgg accgcgggcg tcaacgccgc tccgttcaag
     5401 gaagccgtca acttctacgt cgacctggtc aaggcccatg gcgaggccgg tgcgccgcag
     5461 gcgggcttca ccgagtgcct caacaacatg acgcagggca aggtcgccat gtggtacgac
     5521 gccacctccg ccgccggatc gctcgaggcc gagggctccc cggtcaaggg caagatcggc
     5581 tacgccgccg caccggtcaa ggtcaccaag agctccggat ggctctacac ctgggcctgg
     5641 gcggtccaga aggcctcgaa gaatcaggac aacgcgtgga agttcatctc ctgggcctcg
     5701 agcaaggaat acgaagagct cgtcggcaag cagctcggtt gggccaaggt cccctcgggc
     5761 aagcgcgcct cgacctacag caacgccgac tacctcaagg ccgccggcgc cttcgccgag
     5821 aaggagaagg ccgccatcga ggccgccgat ccgctgaacc cgggcgcaca gcccaggccg
     5881 gccatgggca tccagttcat cggcatcccg gagttcccga agctcggcac cgacgtcagc
     5941 cagctcgtca gcagcgccat cgccggccag accacggtcg acgccgcatt ggacgagggt
     6001 cagaagctcg ccgaggccgt ctccgcgaag tacaagggta agtaagccca tgaccacgac
     6061 ccccaccaaa ggggccgggg tcgcggagac ggagaagaga agcaacctcc agcgctcagg
     6121 agactgggcg cggaggatgc ctctgcttcc cgcgatggtc ctggtcttcc tcgtcacgca
     6181 actgccgttc ctgttcacga tcggcgcctc tttcatgaac tggaacgcct actaccccga
     6241 cgagcgtcgg ttcaacggcg gggcgaactt caagacggtg ctgaccgacc cggccgcccg
     6301 caaggcgatc tgggtgaccg tgctgctgac ggccgccgtc gtgctgatct cgctcttcct
     6361 cgggctgatc atcgcgctcc tgctcgaccg gaagttcttc ggccagggca tcgtgcgcac
     6421 gatgatgatc accccattcc tcgtcgtgcc ggtcgcggcc gccctgttgt ggaagcacgc
     6481 cctctacaac cccgaatacg ggctcttcaa cggcatgctg acgaagttgt tcggcgagaa
     6541 cgccccgcag ccggactgga tcagtgagca cccgatgtgg gcggtgatct tcgccatcgt
     6601 gtggcagtgg acgccgttca tgatgctcat cctgctcgcc gggctgcagt cgcggccgat
     6661 ggatgtcgtc gaggccgcga agatcgacgg cgccagctcg tggcagatct tccgcagtat
     6721 gacgctgccg cacctgcgtc cctacatgga gttgtcgggt ctgctcggaa cgatctatgt
     6781 ggtgcagaac ttcgaccacg tcttcacgat caccggcggc ggcctcggca cggccaacct
     6841 gccgtactac atctacacga ccttctacac cggccaggac tacgggctgg catcggccgc
     6901 cggcgtcgtc gtggtgatcc tgacgatcat cgtctcgacc gtcgccttgc gcaccgtgtt
     6961 cagcatcttc cgggaggagc agcgatgagc ggcaagaccc tgtccaccgg ccaggtggag
     7021 aacgtccaca agaagtcgac ctccggtgtg ttgttgtcga tcgcggcgtg gatcgtcggc
     7081 ctgttgttcg tctctccggt gctctggatg atcctcacga gcttccacag cgagacggac
     7141 gcggccaaga acccgccggc cttcggtgcg ccgctgacgc tggacggtta tcgctccttc
     7201 ttcgacgccg gcccgtggcc gccgttgatc aactccgtaa tggccagcgt cgtctcgacg
     7261 ctcttggtgc tgttgctggc cttccccgcc gcctacgccc tctccatccg gccggtcaag
     7321 aagtggtccg atgtcctctt cttcctgctg tcgacgaagt tcctgcccgt cgtggccggc
     7381 ctgctgccga tctacctctt cgccaagaag gtcggcatgc tcgacaacat ctggctgctc
     7441 atcctcttct acacggtgat gaacctgccg atcgcgatct ggatgctgcg ctccttcctc
     7501 gcggaggtgc cggtcgagat gctggaggcg gccgaggtcg acggtgccgg gttggtcctg
     7561 accctgcgca aggtcgtggc acccgtggtc atgcccgggc tggcctccgc cgcgctgatc
     7621 tgcttcatct tcagctggaa cgagctgctg ttcgctcgcg tgctcacggg cacggtcagc
     7681 cagaccgcac cggtctacct gaccgggttc gtcaccagcc agggcctctt cttggcccag
     7741 gtgtgtgcgg cctcgctcgt cgtgtcgctg ccggtgctcg tcgccggatt cgcggcgcag
     7801 gacaagctgg tccagggcct gtcgatggga gcggtgcgct gatgggcacg ccgatcccgc
     7861 tcggcaatgc caccctcggg gagctcccct ccgaggtggg cgtgccggca tacgaccgat
     7921 ccgccctgac ccccggcatc gtccacttcg gcgtcggtgg cttccaccgc gcccacgagg
     7981 cgatgtatct cgatgcgctg ctgaacgagg gcaaggcact cgactggggc atcgtcggcg
     8041 tgggcaccat gccgggcgac gcccggatgc gcgatgcgat gaaggcgcag gactgcctct
     8101 tcaccctcgt gctgaagcat cccgacggct cccgggacgc ccgggtcatc ggcgggatgg
     8161 tcgactacct cttcgccccg gacgacccgc aggcggtgct cgagcggctg gccgacccga
     8221 gcacccggat cgtctccctc accgtcaccg agggtggcta ccacgtcaac caggtgacgg
     8281 gggagttcga cccgaacagc cccgacctgc gggccgacct ggccggcgac gccccgccca
     8341 agagcgtctt cggcttcatc accgaggcgg cgcggatgcg gcgcgagcgc ggcataccgc
     8401 ccttcaccgt gatgagctgc gacaacctgc ccggcaacgg ccacatcgcc gagaagatga
     8461 tcacggcgtt cgccgaactc aaagaccccg agctggcgga gcacctgcgc caggacgtcg
     8521 ccttccccaa ctgcatggtc gaccggatca ccccggtcac ccagcaggcc gatatcgacg
     8581 acctcgccga acgcttcggg gtcgccgatc gctggccggt ggtgtgcgag ccgttcaccc
     8641 agtgggtgct cgaggacaac ttcggcgccg ggcgaccggc gttcgaagag gtcggcgtcc
     8701 agatcgtccc ggacgtcgtg ccgtacgagc tgatgaagct gcggctgctc aacgcctccc
     8761 accaggcgct gtgctatctc gggtatctca gcggctaccg ctacgcgcac gaggtctgcc
     8821 aagacccgct cttcgtgaag ttcctcctcg gctacatgga gaacgagggc agcccgacac
     8881 ttcccgaagt gccgggggtc gatctggacg cctaccgcca ccagctcatc gagcgcttcg
     8941 ccaaccccga agtgcgcgac acgctggcgc gcctgtgcgc cgagagctcg gaccgcatac
     9001 ccaagtggct ggtgccggtg atccaggaga acctgcgcaa cggtcgggac atcgaccggt
     9061 ccgcgctggt cgttgcgtcc tgggcccggt atgccgaggg tgtcgacgag cagggtgacc
     9121 cgatcgacgt cgtggaccgc ttcaaggaca aggtgatggc ggccgcagcg gcccagcagg
     9181 atgacgatct ggccttcctg cgcgacaagg acttcttcgg aaacctcgtc gacgacgaac
     9241 gcttcaccac cgcataccgc gggttcctcg cgagcctgca cgagcgcggc gcccgcgcca
     9301 ccctcgagga actcgagggc aggtagcgct cacagtcgcc ggcagccgga gtcccgacct
     9361 ggggggctcc ggctgccgtg cataactagg atcgacacac caggaaccga tggaggtcac
     9421 catgacgcgc tcgctcgtgg cgggggtcga cagctcgacc cagtcgtgca agatcgtcgt
     9481 ctgcgacgcc gagaccgggc aggtcgtgcg atccgctcgt gccccgcacc ccgacggcac
     9541 cgaggtcgac ccggcggcct ggtgggcggc ctggcaacag gcgagctcgg gtggactgct
     9601 cgacggggtc gaggcgatcg ccgtcggcgg gcagcagcac ggcatggtga ccctggacga
     9661 tgcggatcag gtggtccggc cggcgctgct gtggaacgac acccgctccg ccggggccgc
     9721 cgccgacctg gtcgaggagt tcggcggcgc gcaggcctgg gccgaggaga tcggcatcgt
     9781 gccggtcgcc gccatcacgg caacaaaact gcggtggctg gcccgcaacg agccggacaa
     9841 tatggcgcgc acgcgcacct gcgtgctccc acacgactgg gtcaccggcc agatcctcaa
     9901 ggacggcaac ggttttcagc agtgggtgac cgatcggggc gacgcctccg gcaccggcta
     9961 ctggtcggcc cacagccagg actatcgcac cgacgtcatg gacctggcga tgggccgcac
    10021 catcgaggtg ccgaccgtgc tcgcccccca cgaggcagcc ggccgcaccg actccgggat
    10081 gatcgtcgcg ccgggcaccg gcgacaacat gggcgcggcg ctcggactcg gcctggcccg
    10141 cggtgacgtc gtcgtctccc tggggacctc gggaaccgtc ttcgcgcggc ataccgaccc
    10201 ggtcgcggac gcgagcggtg ccctgcagtc cttcgccgac gcggagggcg gctacctgcc
    10261 gctgctgtgc acgctcaatg cgtcgcgggt catcaacgcc ggcatggcga tgctcggggt
    10321 ggactactcc gagttcgacc ggctggccat ggccgctgcg cccggggccg gcgggctgac
    10381 gctcattccc tacctggacg gcgagcgcac cccgaacctg cccgacgcca cgggcaccct
    10441 gctcgggctg cagcgtgcca acgccactcc cgagaacctc gcccgcgcgc acgtcgaggg
    10501 tatgctgctc aatctcgttg cgggagtaag cgctttgcgc gaccacgggg ttgcggtcga
    10561 gcggatcctg ttgatcggcg gggcggctgc gtcgacggcc gttcaggaca tcgcctgcca
    10621 gctcttcggg ttgcccttgg tcgtgccggc ggcgggggag tatgtcggtc tcggggctgc
    10681 ccggcaggcc gcgtggaccc tgtcgggtgc ggacgcgccg ccgcagtggg aggtctcgct
    10741 cgatgtccgc gcagagccgg gcgcgccgga tgccggggcg gagatcgtcg aacgctatcg
    10801 gcaggccgtg cgcgaccagc acggcgtcgg ctgacacacg cgggagtgcc ggcgtggcgg
    10861 aacaggtgac tcttaagacc ccacaagggc cgggccgggc ccacgtctat agcggctcgg
    10921 tgccggtgcg ggcgaccgtc gtgctgggcc acggcgccgg gggtggcatc ggcgcgagcg
    10981 acgtgatgct gctcgcgtcg cggctgccgg agcacggcta ccgggtggtg ctcatcgagc
    11041 agccgtggaa ggtcgccggc cgcaaggtgg cgggtcgtcc gccgacgctc gatgcggcgt
    11101 ggatccccat gctcgggcaa ctggacgtcg tgggctcgct ggtcgtcggc ggccgcagcg
    11161 ccggcgcccg agtcgcctgc cgcaccgccg aaaccgttgg cgcgcaaggg gttctgtgct
    11221 tgtccttccc gctccacccg ccgggcaaac ccgacgcctc acgggccggc gagctgcgga
    11281 ctccgggaga acacggcata cccgtgcggg tcattcaggg cgagcgggac ccgttcggga
    11341 ctcccgacga gctgcgcgca gaactcgccg acccggcctg ggtcgccgcg gtggcgggca
    11401 cccattcgtt cggcaagaag ccggccgacg tcctcgacgc cgccatccga ctcctgcccg
    11461 agttgctata gcgcgccgcc ggcggccggg
//
LOCUS       DAOPJG010000105        11429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5941_length_11429_cov_7.535871, whole genome shotgun sequence.
ACCESSION   DAOPJG010000105 DAOPJG010000000
VERSION     DAOPJG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11429
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5941_length_11429_cov_7.535871"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKU75_10325"
     CDS             complement(<1..425)
                     /locus_tag="PKU75_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81318.1"
                     /translation="MSGIGAFARARDGSLIGRSAAESEWIPIAREFLATVASRPYAVT
                     TQRELAQHVLSRSGIEVNPSLNKWIGTFLTHLTQACVAEGEPPLGALVTADHGKVGPA
                     FDEALASLELGAETPKDRERLAAVLRLQCYRWADAPEPAG"
     gene            complement(511..735)
                     /locus_tag="PKU75_10330"
     CDS             complement(511..735)
                     /locus_tag="PKU75_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPF81319.1"
                     /translation="MSHTVGPGRRDSEGTAPGRRRFSTSTDGPFCEAKEIMGGFWVVE
                     ATDDEEAVALATEAQAACGNVIEVRPLQGA"
     gene            793..1875
                     /locus_tag="PKU75_10335"
     CDS             793..1875
                     /locus_tag="PKU75_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HPF81320.1"
                     /translation="MPKPPAAEVIAGDRVVRVSSPDRVIYPATDTTPEVTKVMVAQYF
                     ADVEDGLMRALRDRPTALERWTKGVLPGMTLGTGRPGEKADAFYQKRVPVGAPDYLES
                     CEITFPSGRTALEVCPTEIAVPVWCAHMGTLTFHPWPVRRDDVDHPDELRIDLDPQPG
                     TDFTDAVRVAGVAKDLLDELGIAGWPKTSGNRGVHIYVRIEPRWSFEEVRHAAIGFGR
                     ELEKRDSGVTTAWWKEERGERIFIDFNQNCRDRTIASAYSLRPKPGAPVSTPMTWSEL
                     ADVRDPAAYNLFTVPERLADGDPWAGIDERAFSIDPLLRLYEESGLGEMPFPPDYPKM
                     PGEPPRVQPSKKVAAHWDADGNRIED"
     gene            1893..>2075
                     /locus_tag="PKU75_10340"
     CDS             1893..>2075
                     /locus_tag="PKU75_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF81321.1"
                     /translation="MGLTLLAVAFDAEDPRSVAEFWGALLARELRPDGDGWVLPGSAT
                     QVGLQFVPVRPDTNPGG"
     assembly_gap    2076..2175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2176..2516
                     /locus_tag="PKU75_10345"
     CDS             <2176..2516
                     /locus_tag="PKU75_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF81322.1"
                     /translation="GTGRLGEVTCDGTQAVGHFWSSALGWPLVWDQDEETAIQSPAGG
                     TKIAWGGPPMAAKRGRNPQHFVLEVGAAEREREVARLTGLGARHTSPPSDGPAELTDP
                     DGNEFVVRAR"
     gene            complement(2527..3690)
                     /locus_tag="PKU75_10350"
     CDS             complement(2527..3690)
                     /locus_tag="PKU75_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF81323.1"
                     /translation="MLIPSPFTPGDLPRVLVGRAAERRRLADLLARVTAYGELAGPPV
                     VLHAPRGLGKTTLLRDTEDRARELGFVTAWVACAKGSPMLTELAASVRRALIDAEVLD
                     DRSTKRVLDTISVEFAIFGVGFSTEFARGAAPEVPTGAISSLERMLHEAAAAIRARGG
                     AGLLVLIDELHAAPYAEVGVLLNAMQNLAGRRAENPLALMTAGLPSTPEMIMRAATFG
                     ERSEFIGIDRFDDADSRRVLEEPAQEEGVGWAADAISAGLDIADGNPYLLQLVGDSTW
                     QAAAPRTGDTLELDDLHAGEDRMRGQLAAMCRARWDSASDLEQAFLGAMADYGEEIVP
                     REHIATTMGRESRAISVPRARLIDRGVIEAAGRGRLRFTLPGMGAWIREYQRG"
     gene            complement(3861..5003)
                     /locus_tag="PKU75_10355"
     CDS             complement(3861..5003)
                     /locus_tag="PKU75_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HPF81324.1"
                     /translation="MPDAIEVRKVPIHSVADASELQKLLDDGVMEASRVIAIIGKTEG
                     NGGVNDYTRIIADRAFREVLMANGAPEEQVKQVPIVWSGGTDGVISPHATIFATVPAD
                     KTEKTDQPRLTVGFAMSEHLLPEEIGYVAMVDKVADAVKVAMERAGITDPADVHYVQT
                     KTPLLTIHTIRDAKSRGKSVWTEHTHESMDLSNGCTALGIAKALGEVDSVSDEDVMHN
                     RAKYSSVASCSSGVELDQAQVVVVGNAKGIGGRYRIGHSVMNDALDAGGIWEAIKDAG
                     LDLPERALSSDIDGRLVNVFLKCEASTDGQVHGRRNAMLDDSDVHWHRQIKSCVGGVT
                     ASVTGDPAVFVSVSAAHQGPDGGGPVAAIVDLGEGEPTGYRWSPSA"
     gene            complement(5084..6028)
                     /gene="arcC"
                     /locus_tag="PKU75_10360"
     CDS             complement(5084..6028)
                     /gene="arcC"
                     /locus_tag="PKU75_10360"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPF81325.1"
                     /translation="MRIIVALGGNAMTAADGSATPQAQIDAIATAMTHVAELVVRGHD
                     VVLTHGNGPQVGNLLVKNELAANVVPPVPLDWCGAQTQGTIGFTVLNSLEAALRRHGV
                     ERPVAALISRTLVDGDDEGFATPTKPVGRYLSHDEAKVLIDHGQTWEDRGEKGWRRVV
                     ASPEPLEVLETGTITTLLDAGYLVVAAGGGGIPVVRDSAGELHGVEAVIDKDLTAAVL
                     AKALDADVLVIATDVENVMVGWGTPQERALEAVTVAEMRQYAADDEFASGSMGPKVEA
                     ALRFIDEGGRCSVITSLDKIGDAIDPDADDVGTVITPG"
     gene            complement(6128..7651)
                     /locus_tag="PKU75_10365"
     CDS             complement(6128..7651)
                     /locus_tag="PKU75_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPF81326.1"
                     /translation="MSDRETRDKPRRLGARELLDTVLDDGSWTSWDGPPLAVARPGSE
                     YEAELAAAAEKAGTDESIITGEGRIGGRRVAIIAGEFRFLAGSIGRTAAERIVLAFER
                     ATREGLPMFATPSSGGTRMQEGTPAFATMVKITSAVAVFKAAKLPYITYLRHPTTGGV
                     FASWGSLGHVTVAEPGATVGFLGSRVFEALYGQPFPTGVQTAENLYDNGLLDAVLPVE
                     VLATQASRFLHLLLEPRHASPIPPVAKEALADIPAWESITRSRRPDRPGVRDLLKLGA
                     SEVIPLHGTGAGETDRSLFLALARFGAQPCVVLGQDRSRQTPDKPLSPSGLRVARRGM
                     RLATDLDLPLVSIIDTSGAALSKEAEEGGLAGEIARSLAEMVVQSQPTLCVLLGEGTG
                     GGAIALMPADRVIAAQHSWLSPLPPEGASAILFRDTSHAPDLATQQHVRSLDLLAEGI
                     VDRIVAERPDAADEPEEFCARMAQAIAGELSDLVAMRPASRLAARHDRYRRIGTVLR"
     gene            complement(7644..8810)
                     /locus_tag="PKU75_10370"
     CDS             complement(7644..8810)
                     /locus_tag="PKU75_10370"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPF81327.1"
                     /translation="MSGPLDGITVIDLSRALAGPQAGMMLGDLGARVIKIESETGDDT
                     RGWGPPFVGPDHDISTYFLAANRNKESIVLDLKSHDGRDILTRLAERADVLVENFRPG
                     VLARLGFSNEHLHELNPRLIVLSISGFGHDGPEGGRAGYDQIAQGEAGLMSVTGSAPD
                     DPQRVGVPIGDLLSGMNGAFGVVAALYERERTGKGRVVRTSLLASVIGAHAMHGTNYL
                     VGGKVGHAQGNHHPSIAPYGLFQADDGPIQIACAAESLWVKFANAFGLDPAAEGMATN
                     RERVHNRDAVVAAVNAALAGHTSAEVLAKLAEIGVPCGHVRTIDQVYTWDQVASQHLL
                     LKVDHPVLGEITIPGSPLRFDDNAYSGGREAHLPPPGLGEHNESIRAWLADSDE"
     assembly_gap    8968..9067
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9160..9480)
                     /locus_tag="PKU75_10375"
     CDS             complement(9160..9480)
                     /locus_tag="PKU75_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81328.1"
                     /translation="MDATVAFYAALGFDVGFRDEAWLVLYRGDLMLEFFPFPEVDPQT
                     SSFSCCLRVDDLDALVAACEPVAPVADRGWPRLHLPTVESSGLRIGYLVDPDGTLLRL
                     IHNS"
     gene            complement(9554..10387)
                     /locus_tag="PKU75_10380"
     CDS             complement(9554..10387)
                     /locus_tag="PKU75_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HPF81329.1"
                     /translation="MTEVLVTGAAGKTGRAVTKALVCRGARVRCAVRPGSSTPIHEDA
                     AARRVPVDLATGEGLGVALAGVDAVYVIAPNVNAREVEMVRHIADAAVAAGIPRVVYH
                     SVMHPDDTAMPHHLRKHEAEKLLREKPFGLTILRPAAYHQNLRPAALAGEIAVPYSLD
                     VPFTNVDLDDIAQVAATAVLTRELDHAAYDLAGPQELSVRELAAIAADVLGRPVRATQ
                     ISREEWLAGPGAALAPQPRADLLAMFTAYDREGFAGDSTILPRLLGGAPSTWENVLSV
                     A"
     gene            complement(10384..>11429)
                     /locus_tag="PKU75_10385"
     CDS             complement(10384..>11429)
                     /locus_tag="PKU75_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HPF81330.1"
                     /translation="FTGTAPQAKGPINHCGDYVGGNFLKKWLAPILRNLADTPERMAE
                     LDVNEGIVPLIEMRFPPKGTLLTPIYPAPTNARTFVILRLLGVLAGVVAKAVDGQMPA
                     DQETIRYTGVYGKDMDDNDYLMREVLGGGSGGRYYADGEDTIHVVPDSRNLPTEFTES
                     RFPFIVERLGLAVDSGGAGRYRGGLGYEKHLRMLKDAHFMSIADRSILACWGVKGGKA
                     GRPFEVTIDLGGPDERMVDALADAEVVEAGQIIRIRTTGGGGWGDPLERPYDEVERDL
                     RWGKVSFDGARESYGVVAGGTKDDVTIDAAASDALRAEMRSARGEEAFFDRGPGYARL
                     AADGANANEFDWL"
BASE COUNT         1671 a         3837 c         3964 g         1757 t
ORIGIN      
        1 cccgccggct cgggtgcatc cgcccagcgg tagcactgca gccgcagtac ggccgcaagt
       61 cgctcccgat ccttgggggt ctccgcaccg agctccagcg acgcgagcgc ctcgtcgaat
      121 gccgggccga ccttgccgtg atcggccgtc acgagggcgc cgagcggggg ctccccctca
      181 gcgacacagg cctgcgtcag gtgcgtgagg aaggtcccga tccacttgtt gaggctggga
      241 ttgacctcga tcccgctgcg cgacaggacg tgctgcgcca actcgcgctg ggtggtcacg
      301 gcatacggcc ggctcgcaac cgtggcgagg aactcccgcg cgatcgggat ccattcgctc
      361 tcggccgcgc tgcgcccgat gagggaacca tcgcgagccc gggcgaatgc cccgatcccg
      421 ctcaccgccg ggcttccgat cgatccacga ccgcaagcct acgtcggccc ggtggccggg
      481 agttgacggc gacgcgtcca ccgggcgacg tcaggcccct tggagcgggc gcacctcgat
      541 gacattgccg caggcagctt gggcctccgt ggcgagggcc acggcttctt cgtcgtcggt
      601 ggcctcgacg acccagaaac cgcccatgat ctccttggcc tcgcagaacg gcccgtccgt
      661 gcttgtcgag aatcggcgcc ggccgggtgc cgtgccttca ctgtccctac gaccgggccc
      721 gaccgtatgc gacagctcgc ctcacgtttg atccgacccg gagcggcggt gggtccccgt
      781 agtctgggcc acatgcccaa gccgcccgca gccgaggtca tcgccggcga ccgtgtcgtg
      841 cgggtttcct cccccgaccg ggtcatctat ccggccaccg acaccacccc cgaggtgacc
      901 aaggtgatgg tggcgcagta tttcgcggac gtcgaggacg ggctgatgcg ggcgctgcgc
      961 gatcggccga cggccctgga gcggtggacc aagggggttc ttccgggtat gacgctcggc
     1021 accggccggc cgggcgagaa ggcggatgcg ttctaccaga agcgggttcc ggtcggcgcg
     1081 cccgactatc tcgaaagctg cgagatcact ttcccttctg gccgaacggc tttggaggtg
     1141 tgtcccaccg agatcgccgt gccggtgtgg tgcgcgcaca tgggcacgct gaccttccac
     1201 ccgtggccgg tgcgccgcga cgacgtggac caccccgacg agttgcgcat cgacctcgat
     1261 ccgcagccgg ggactgactt caccgatgcg gtgcgggtcg ccggtgtcgc caaggacctg
     1321 ctcgacgaac tcgggatcgc cggctggccg aagacgtccg gcaaccgggg cgtgcacatc
     1381 tacgtccgca tcgagccgcg ctggagtttc gaggaggtcc ggcacgccgc gatcggcttc
     1441 ggccgcgagt tggagaagcg cgactcgggc gtcaccaccg cgtggtggaa agaggaacgg
     1501 ggagaacgca ttttcatcga cttcaaccag aactgccggg accggacgat cgcctcggcg
     1561 tactccctgc gacccaagcc gggtgccccg gtctcgacgc cgatgacgtg gagcgagctt
     1621 gctgacgtgc gcgaccccgc ggcatacaac ctgttcacgg tgcccgagcg actcgccgac
     1681 ggcgacccgt gggccgggat cgacgagcgc gccttctcga tcgacccgct cctgcgcctg
     1741 tatgaggagt ccggcctggg agagatgccg ttcccgcccg actatccgaa gatgcccggg
     1801 gagccgccgc gggtccagcc gagcaagaag gtcgccgccc actgggacgc cgacggcaac
     1861 cgcatcgagg actgatcgag gagtatgacg cggtgggact gacgctgctc gccgtggcct
     1921 tcgacgccga ggacccgcgc tcggtcgcgg aattctgggg agcccttctg gcgcgtgagc
     1981 tgaggccgga cggtgacggg tgggtgctgc ccgggtcggc gacccaagtg ggactgcagt
     2041 tcgtcccggt gcgccccgac accaatcccg gcggcnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnncgggc accggccggc tgggcgaggt gacgtgcgac ggcacgcagg
     2221 cggtgggcca cttctggagt tccgcactgg gctggccgct ggtgtgggac caggacgaag
     2281 agaccgccat ccagtcgccc gccggcggga cgaagatcgc ctggggcggc ccgccgatgg
     2341 ccgccaagcg tggacgcaac ccacagcact tcgtcctgga ggtcggcgcc gctgagcggg
     2401 agcgcgaagt cgcccggctg accgggctgg gggcacggca taccagccct ccgagcgacg
     2461 gaccggcgga gctcaccgac ccggacggca acgaattcgt cgtccgcgcg cgctgacgaa
     2521 ttgtgttcag ccgcgctggt attcgcggat ccaggcgccc atccccggca gggtgaagcg
     2581 cagcctgccg cggccggccg cctcgatgac gccgcggtcg atgagccggg cccgcggcac
     2641 gctgatggcc cgtgactccc ggcccatggt cgtcgcgatg tgctcgcgcg ggacgatctc
     2701 ctcgccatag tcggccatgg ccccgaggaa ggcctgctcc agatcgctcg cggagtccca
     2761 tcgcgcgcgg cacatggcgg ccagctgacc gcgcattcgg tcctcgccgg cgtgcaggtc
     2821 gtcgagctcg agcgtgtccc ccgtgcgggg cgcggcagcc tgccacgtgg agtccccgac
     2881 gagctgcagc aggtaggggt tgccgtcggc gatatcgagg cccgcggaga tcgcatccgc
     2941 cgcccacccc accccctcct cctgcgccgg ctcctccaac acccgccgcg aatccgcgtc
     3001 gtcgaaacgg tcgatcccga tgaactcgct gcgctcgccg aaggtcgccg cgcgcatgat
     3061 catctccggc gtggacggca gccccgcggt catcagcgcg aggggattct cggcgcgccg
     3121 gccggcgagg ttctgcatgg cgttcaggag gactccgacc tcggcatacg gtgcggcgtg
     3181 caactcgtcg atgagcacga gcaggcccgc gcccccgcgc gcccggatcg ccgcggccgc
     3241 ctcgtgcagc atccgctcga ggctggagat cgcgccggtc ggcacctcgg gcgccgcccc
     3301 gcgcgcgaac tccgtcgaga agcccacccc gaagatcgcg aactccaccg agatggtgtc
     3361 gagcacccgc ttggtcgacc ggtcgtcgag cacctcggcg tcgatcaacg cccgccgcac
     3421 gctcgccgcc agctcggtga gcatcgggct ccccttggcg caggccaccc acgcggtgac
     3481 gaaccccagc tcgcgggccc ggtcctcggt gtcgcgcagg agtgtcgtct tgcccaggcc
     3541 acgcggcgcg tgcaggacga ccggtggccc cgccaactcg ccgtatgccg tcacccgcgc
     3601 cagcaggtcg gccagccggc ggcgctcggc ggcccggccg acgagcaccc ggggcaggtc
     3661 accgggggtg aagggactgg ggatcagcac tacccacctc gcgaggatcg tttgttatac
     3721 atcgtttgtt atacatcggc tgtctataac aatcgctatc gacgatcgcc actctcccat
     3781 gcggcaccga caatgacccc acgacgcgtg cgacggttgg cgaccatagg ccgccaaccg
     3841 tcgtgcttgg tgagacgccg tcaggcggag gggctccagc ggtagccggt gggctcgccc
     3901 tcgcccaggt cgacgatcgc ggccaccggg ccaccgccgt cggggccctg gtgcgcggcg
     3961 gagacggaga cgaacaccgc cgggtcgccg gtcaccgatg cggtgacgcc gccgacgcag
     4021 ctcttgatct gccggtgcca gtgcacgtcg gagtcgtcga gcatggcgtt gcggcggccg
     4081 tggacctggc catcggtgga tgcctcgcac ttgaggaaga cattgacgag gcggccatcg
     4141 atgtcgctgg acagcgcccg ctcgggcagg tcgaggcccg cgtccttgat ggcctcccag
     4201 atgccgccgg cgtcgagggc gtcgttcatg accgagtgcc cgatccggta gcggccgccg
     4261 atgcccttgg cgttgccgac cacgacgacc tgggcctggt cgagttcgac cccggaagag
     4321 caggaggcga ccgaggaata cttggcccgg ttgtgcatga cgtcctcgtc gctcaccgag
     4381 tcgacctctc cgagggcctt ggcgatgccg agggcggtgc agccgttgga gaggtccatc
     4441 gactcgtgcg tgtgctcggt ccagacgctc ttgccgcgcg acttcgcgtc gcggatggtg
     4501 tggatggtca gcagcggcgt cttggtctgg acgtagtgca cgtcggccgg gtcggtgatg
     4561 ccggcccgct ccatcgcgac cttgacggcg tcggccacct tatcgaccat cgcgacatag
     4621 ccgatctcct ccggcagcag gtgctcgctc atggcgaacc cgaccgtcag ccgcggttgg
     4681 tcggtcttct cggtcttgtc cgcgggcacg gtcgcgaaga tcgtcgcgtg cgggctgatg
     4741 acgccgtcgg tgccgccgga ccagacgatc ggcacctgct tgacctgctc ctcgggggcg
     4801 ccattggcca tcagcacctc gcggaaggcg cggtcggcga tgatccgggt gtagtcgttg
     4861 acgccgccat tcccctccgt cttgccgatg atggcgatga cgcggctggc ctccatgacg
     4921 ccgtcgtcga gcagcttctg cagctcggac gcgtcggcga cggagtggat cgggaccttg
     4981 cgtacttcga ttgcgtcagg cacttcgggt gacctttctg tcggtgctca cattggcgtt
     5041 ttcgttgctg ggtatgctgt tcggctcggc ttgggtggcc cggtcacccg ggcgtgatga
     5101 ccgttccgac gtcgtcggcg tcgggatcga tggcgtcccc gatcttgtcg agggaggtga
     5161 tcacactgca ccggcccccc tcgtcgatga accgcaacgc cgcctcgacc ttcggcccca
     5221 tggagccgct cgcgaactcg tcgtccgcgg catactgtcg catctccgcg accgtcacgg
     5281 cttcgagggc ccgctcctgc ggcgttcccc atccgaccat cacgttctcg acgtcggtgg
     5341 cgatgacgag cacgtcggcg tcgagggcct tcgccagcac cgcggccgtg aggtccttgt
     5401 cgatgacggc ctcgacaccg tggagctcgc cggcgctgtc gcgaaccacc ggtatgccgc
     5461 cgccccccgc cgcgacgacg aggtagcccg cgtcgagcag ggtcgtgatc gtcccggtct
     5521 cgagcacctc gagcggctcc ggtgaggcga cgacgcggcg ccagcccttc tcgccccggt
     5581 cctcccacgt ctggccatgg tcgatgagaa ccttcgcttc gtcgtgcgac aagtagcggc
     5641 ccaccggctt ggtcggggtg gcgaatccct cgtcgtcgcc gtcgacgagg gtgcgcgaga
     5701 tcagggcggc gacagggcgc tcgacgccgt ggcggcgcag tgccgcttcg aggctgttga
     5761 ggacggtgaa gccgatcgtg ccctgcgtct gggcgccgca ccagtcgagc ggaacaggcg
     5821 gcacgacatt ggcggccagc tcgttcttga cgagaaggtt gcccacttgc gggccgttgc
     5881 cgtgggtgag cacgacgtcg tgtccgcgga ccacgagttc ggcgacatgc gtcatcgcgg
     5941 tggcgatggc gtcgatctgc gcctgcggcg tggcgctgcc gtccgctgca gtcatggcgt
     6001 tgccgccgag ggcgacgatg atcctcacgc gtcgaagcct aggaatgtgg tcccggactc
     6061 ggcgttgtca gcacctgccc acgagtcggc ctttcgttgg cacattgcca tgtatgccgc
     6121 acccatgtca ccgcaggacg gtgccgatgc ggcggtagcg gtcgtggcgg gcggcgagcc
     6181 gggaggcagg gcgcatggcg acgagatcgg acaattcgcc ggcgatcgcc tgcgccatcc
     6241 gggcacagaa ctcctcgggt tcgtcggcgg catccgggcg ctcggcgacg atccggtcga
     6301 caatgccttc cgccagcagg tcgagggatc ggacatgttg ctgggtcgcc agatcggggg
     6361 cgtggctcgt gtcccggaag aggatggcgc tcgcgccttc cggcggcagc ggcgacaacc
     6421 acgagtgttg cgcggcgatg acccggtccg cgggcatgag cgcgatcgcg ccaccgccgg
     6481 tgccttcacc gagcaggaca cacagggtgg gttgggactg gacgaccatc tcggccagcg
     6541 accgggcgat ctcgccggcc aggccgccct cctcggcctc cttcgacaaa gccgcgccgg
     6601 aggtgtcgat gatcgagacc aggggcagat cgaggtcggt ggcgagccgc ataccgcggc
     6661 gggccacgcg aagtccgctg ggagacaagg gcttatcggg cgtctggcgg ctgcggtcct
     6721 gaccgaggac gacgcagggt tgcgcgccga accgggcgag cgccagaaaa agggagcggt
     6781 ccgtttcgcc cgcacccgtg ccgtgcagcg gaatcacttc cgacgcaccg agtttcagca
     6841 ggtcccgaac gccggggcgg tccgggcggc gagatcgggt gatcgactcc catgcgggga
     6901 tgtcggcgag cgcctctttc gcaaccggtg ggatcggcga ggcgtggcgc ggctcgagca
     6961 gcaggtggag gaagcgactg gcttgtgtcg caaggacttc cacgggcagc acggcgtcga
     7021 gcaggccgtt gtcatagagg ttctcggccg tctgcacccc agtggggaag ggctgcccat
     7081 agagcgcctc gaaaacccgc gagccgagga agccgaccgt ggccccgggc tcggcgacgg
     7141 tgacatggcc gagggagccc cacgaggcga agacgccgcc ggtcgtcggg tgccgcagat
     7201 aggtgatgta gggcagtttg gccgccttga acacggcgac ggccgaggtg atcttgacca
     7261 tcgtcgcgaa agccggtgtg ccctcttgca ttcgggtgcc acccgaggac ggggtggcga
     7321 acatcggcag cccctcgcgc gtggcgcgct cgaaggcgag cacgatgcgc tctgcggcag
     7381 tgcgcccgat ggatccggcg aggaagcgga actccccggc gatgatcgcg acccggcgac
     7441 cgccgatccg gccctcgccg gtgatgatcg actcgtccgt gccggccttt tccgcggccg
     7501 cggcgagctc ggcctcatac tcgctgccgg gccgggcaac ggcgagtgga ggcccgtccc
     7561 acgacgtcca cgacccgtcg tcgaggacgg tgtcgaggag ttcgcgggcc ccgaggcggc
     7621 gtggtttgtc gcgggtttcc ctatcactca tcgctgtccg ccagccaggc gcggatcgac
     7681 tcgttgtgct ccccgagccc cgggggcggc aggtgcgcct cgcgcccacc cgaataggca
     7741 ttgtcgtcga aacgcaacgg cgagccgggg atggtgatct cgccgagcac cgggtggtcg
     7801 accttcagca acaaatgctg gctcgcgacc tggtcccagg tgtagacctg atcgatcgtg
     7861 cgcacgtgac cgcaggggac gccgatctct gcgagcttcg cgagcacttc cgcgctcgtg
     7921 tgacccgcga gtgcggcgtt gacagcggcc acgacggcgt cgcggttgtg cacccgctcg
     7981 cggttggtcg ccataccctc ggcagcgggg tcgaggccga acgcattggc gaacttgacc
     8041 cacaggctct cggcggcaca ggcgatctgg atcggaccgt cgtcggcctg gaagagtccg
     8101 tagggcgcga tcgacgggtg atgattgccc tgggcgtgac cgactttgcc gccgacgagg
     8161 tagttggtgc cgtgcatggc gtgggcaccg atgaccgagg cgagcaggct ggtgcgcacg
     8221 acgcgcccct tgccggtgcg ttcgcgctcg tagagcgccg cgacgacgcc gaaggcgccg
     8281 ttcatgcccg acagcaggtc gccgatcggg acgccgaccc gctgcgggtc gtcgggcgcg
     8341 gagccggtca cggacatcag gccggcttcg ccctgggcga tctggtcgta tccggcgcgc
     8401 ccgccttcgg gtccgtcgtg accgaaaccg ctgatggaca agacgatcag tcgcgggttc
     8461 agttcgtgca ggtgctcgtt cgagaaaccg agccgggcca ggactcccgg ccggaagttc
     8521 tcgacgagca catcggcgcg ctcggcgagc cgagtcagga tgtcgcggcc gtcgtgggac
     8581 ttcaggtcca gcacgatcga ttccttgttg cggttggccg ccaggaaata ggtcgagatg
     8641 tcgtggtccg gaccgacgaa cggcggcccc cagccgcggg tgtcgtcacc ggtctcggac
     8701 tcgatcttga tcacccgcgc cccgaggtcg ccgagcatca tcccggcctg cgggcccgca
     8761 agggcacggg agagatcgat gacggtgatg ccgtcgagag gaccggacac ggtgggctcc
     8821 ttcgctcgcc aggtccttcg agcctagagc gctctcccgc tccgaccggc gtcaggttct
     8881 ggcaactccc gtcacacaac gctggcagga gttgcccacc acactcagtt gatgatcagc
     8941 gctgtcacga catgggccag ctgccccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnacg gcgcccgccc tgaggtgtca ccagttgggg ttcctccggc gtggaatggc
     9121 ccaactcgtg acaggccggt cgccccggga tctgagcgat caggagttgt ggatcaggcg
     9181 cagcagcgtc ccgtcgggat cgacgaggta gccgatccgc agcccggacg actcgacggt
     9241 cggcaggtgc agccgtggcc atccgcggtc ggccaccggc gcgaccggct cgcaggccgc
     9301 caccaaggcg tccagatcgt cgacccgcag gcagcagctg aacgaactgg tctgcgggtc
     9361 cacctccggg aaggggaaga actccagcat cagatccccg cgatagagga ccagccaggc
     9421 ctcgtcccgg aagcccacgt cgaatcccag tgcggcatag aacgcaaccg tggcatccat
     9481 atcgcgcgtc ggcaggtttg gagtcgcacg atccaccgtt tcagcatatc cccgccgctt
     9541 tgtgggcgaa cggtcatgcg acggaaagca cgttctccca cgttgacggc gcgccgccga
     9601 gcaggcgcgg gagaatggtg gagtcgccgg cgaatccttc gcggtcgtat gcggtgaaca
     9661 tcgccagcag gtcggcgcgc ggctgcgggg cgagagccgc cccggggccg gcgagccatt
     9721 cttcgcggct gatctgcgtg gctcgcacgg gccggccgag cacgtcggcg gcgatggcgg
     9781 ccagttcgcg cacgctgagt tcctgcggcc cggccaggtc atatgcggca tggtcgagct
     9841 cgcgcgttag caccgcggtg gctgccacct gtgcgatgtc gtcgaggtcg acattggtga
     9901 agggcacgtc gagcgagtag ggcacagcga tctcgccagc aagcgcggcg ggccggagat
     9961 tctggtggta tgccgcgggc cgcagaatcg tcagcccgaa cggcttctcg cgcaacagct
    10021 tctcggcctc gtgcttgcgc aggtgatgcg gcatggccgt gtcgtccggg tgcatcacgg
    10081 agtgatagac cactcgcggg atgccggctg ccaccgcagc atccgcgatg tgccgcacca
    10141 tctccacttc gcgcgcattg acattaggcg cgatgacata gacggcgtcg acgccggcca
    10201 atgcgacgcc gaggccctcc cccgtcgcga gatcgaccgg cacccggcgc gcggccgcat
    10261 cctcgtgaat cggcgtcgat gagccgggcc gcacggcgca gcggacgcga gccccacggc
    10321 ataccagagc cttcgtgacg gcgcggccag tcttgccggc cgcacccgtc acgagcacct
    10381 cggtcacagc cagtcgaact cgttggcgtt ggctccgtcc gcggccagcc gggcatagcc
    10441 cgggccacga tcgaagaagg cctcttcgcc acgggcagaa cgcatttcgg cgcgcagcgc
    10501 atcactggct gccgcgtcga tcgtgacatc gtccttggtg ccgccggcga cgacgccata
    10561 ggactcgcgg gcgccgtcga aggagacctt gccccagcgc aggtcccgct ccacttcgtc
    10621 atagggccgc tcgagcgggt cgccccagcc gccgccgccg gtggtgcgga tgcggatgat
    10681 ctgcccagcc tcaacgacct cggcgtcggc caacgcgtcg accatgcgct cgtccggccc
    10741 gccgaggtcg atggtcacct cgaagggccg gccggccttg ccacccttga cgccccagca
    10801 cgcgaggatc gagcggtcgg caatggacat gaagtgcgcg tccttgagca tgcgcaggtg
    10861 cttctcgtag ccgagaccgc cccgatagcg gcctgcccca ccggaatcca ccgccaggcc
    10921 caggcgctcg acgatgaacg ggaagcggga ttcggtgaac tcggtcggca ggttgcggct
    10981 gtcggggacg acgtggatgg tgtcctcacc gtcggcgtag tagcggccgc cggagccgcc
    11041 gccgagcacc tcgcgcatga ggtagtcgtt gtcgtccatg tccttgccgt agaccccggt
    11101 gtagcggatc gtctcctggt cggccggcat ctgcccgtcg acggccttgg cgacgacccc
    11161 ggcgaggacg ccgagcagcc gcaggatgac gaaggtgcgc gcgttggtcg gggccggata
    11221 gatcggcgtg agcagagttc ccttgggcgg gaaccgcatt tcgatgagcg gcacgatgcc
    11281 ctcgttgaca tcgagctcgg ccatccgctc gggggtgtcg gcgagattgc gcaggatcgg
    11341 cgcgagccac ttcttgagga agttgccgcc gacatagtcg ccgcagtggt tgatcgggcc
    11401 cttggcctgc ggcgcggtgc cggtgaagt
//
LOCUS       DAOPJG010000106        11362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_5986_length_11362_cov_7.785973, whole genome shotgun sequence.
ACCESSION   DAOPJG010000106 DAOPJG010000000
VERSION     DAOPJG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5986_length_11362_cov_7.785973"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..86)
                     /locus_tag="PKU75_10390"
     tRNA            complement(12..86)
                     /locus_tag="PKU75_10390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Arg,seq:ccg)
     gene            225..791
                     /locus_tag="PKU75_10395"
     CDS             225..791
                     /locus_tag="PKU75_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81331.1"
                     /translation="MSCRHYYLLTLPLAPTTDQAVIDAFRAVAEGGRPSASDLQRVPR
                     FPAEIIAGWDRWLAADAGGARLAGAPVKLARIEGGDGPLALTIEFSIHDDANADGGWV
                     FEYWLYTVCAPPVPGTRQVVGIRQADRWDEPELISLGDDGVRVGERFLSGPTCEPPWP
                     DTSTRPRRDSAVPPGRGGMVAHGPTRAP"
     gene            801..2447
                     /gene="argS"
                     /locus_tag="PKU75_10400"
     CDS             801..2447
                     /gene="argS"
                     /locus_tag="PKU75_10400"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPF81332.1"
                     /translation="MTPEQLAAAIRAALADLVAAGEFDLEVPETLRVERPRQAGHGDW
                     STNVAMQLAKTAGMPPRDFAGKLAGALEQVAGIAAVEVAGPGFINITLDAASAGELAR
                     TIVEAGAAYGQGTSEAGRVVNLEYISANPTGPLHIGHTRWAALGDAMVRLLRAAGAEV
                     AAEYYINDAGGQVAKLGGSVLAKLRGEAAPEGGYDGEYVDGLAEQVRAGRPDILDLPA
                     DEALQAAWDVAYPIQLAAIKQTLADFNVAFDVWFSEKSLHDAGAVEQAVARLREQGHV
                     FDQDGAIWLRTTDFTDDKDRVLIRANGVPTYFAADAAYYLSKKDRGFDEKIYLLGADH
                     HGYIGRLKAIAACAGDDPEHNIEVKIGQLVNIAGERMGKRRGNALYMDELIEWIGPDA
                     LRYSLARYPSDSPLSLDGEEMRRQTNDNPVFYVQYAHARTSNVARLAAEDGVHREDGF
                     DPSLLTDPTEAALLAVLGDFPRVTAQAASLLEPHRVARYLENLAAHFHKWYDTCRVRP
                     MNADEPVTDLHRTRLWLNDATRTVLANGLGLLGVSAPERM"
     gene            2451..3884
                     /gene="lysA"
                     /locus_tag="PKU75_10405"
     CDS             2451..3884
                     /gene="lysA"
                     /locus_tag="PKU75_10405"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPF81333.1"
                     /translation="MRAHEAGVLHADGYGGPPIYLPFPDDVMALTPALWSRTVRRRDD
                     GVLEVGGVSVLDLAREHGTAAYILDEADFRARAGAFRDEYAAAFAHLGGADVYYAGKA
                     FLCSAVARWVMEEGLNLDVCTGGELAVAQRVGFSGERIALHGNNKTLAEIRQALSYGV
                     GRIVVDSFVEIERIVAVATELGVVAPVMLRVTVGVEAHTHEFIATGHEDQKFGFSLAG
                     DVAFNGVREILAHPGQLRFLGLHSHIGSQIFDTAGFETAARRLVRLHHRIADELGCAA
                     PEIDLGGGYGIAYTSQHTPLSTAVLAEEMSHIVEQAFKTVGDGSLDDVPRVSIEPGRA
                     IVGPAMFTLYEVGTVKPVTVADGHVRTYVSVDGGMSDNARPALYDADFSVTLASRASS
                     AAPLVVRVVGRHCEAGDIVVQDEWLPADVTPGDLLAVPGTGAYCRSLSSQYNHTPRPP
                     VIAVRNGESRVLIRRETIDDLLGLDVG"
     gene            3887..5224
                     /locus_tag="PKU75_10410"
     CDS             3887..5224
                     /locus_tag="PKU75_10410"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPF81334.1"
                     /translation="MSEHTNPLGDKPIRVALLGCGVVGSSVARMLVESADDLRARIGA
                     PVELVGIAVRRVGKDRSDVPVDPALFTTDAEDLVTRADIVVEVVGGIDPARGLLLKAM
                     ESGASVVTANKALLAEDGPTLYAAADAAGVDLYYEAAVAGAIPILRPMRESLAGDDVT
                     KVLGIVNGTTNFVLDKMDTTGAGFEETVAQAQALGYAEADPTADVEGFDAAAKAALLA
                     SLAFHTRVKGADVFREGISDVTAADIAAAKAMDHVVKLLAICERTTGQNGTSGVSVRV
                     HPAMIARTHPLASVRDAYNAVFVEASAAGQLMFYGRGAGGDPTASAVLGDLVAVARHI
                     VAGGRGPGESAYADLPILDVGDSVTRYHISLDVEDRPGVLAQVAQAFADTGVSIETVR
                     QHIRTNSSSGEARANLVIVTHAATEAALAATVDALAASEVVAEVVSVMRVEGA"
     gene            5230..6327
                     /gene="thrC"
                     /locus_tag="PKU75_10415"
     CDS             5230..6327
                     /gene="thrC"
                     /locus_tag="PKU75_10415"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPF81335.1"
                     /translation="MAHQWRGVMREYADRLPMLDGAPVITLHEGGTPLIAAEKLSDRV
                     GARVFVKYEGLNPTGSFKDRGMTTAISMAAKHGAKAVICASTGNTSASAAAYATKAGM
                     TCAVLVPDGKIAMGKLSQAIAHGATLLQVEGNFDDCLTVARKLAEVYPVDLVNSVNPA
                     RIEGQKTASFEIVDALGDAPDIHCLPVGNAGNITAYWKGYREYVAGHGIPDVPDGPAT
                     KRPQMWGFQAAGSAPIVLGHPVDQPETIATAIRIGNPASWQQAEEARDDSGGRIEAVT
                     DQQILEAHRLLSSTEGIFVEPASAASVAGLLQVHAAGGVPEGATIVCTVTGHGLKDPD
                     WALKGLDGEPVAPQRIPVDVYGAAVALGLDG"
     gene            6342..7304
                     /gene="thrB"
                     /locus_tag="PKU75_10420"
     CDS             6342..7304
                     /gene="thrB"
                     /locus_tag="PKU75_10420"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPF81336.1"
                     /translation="MPGTTWRAGHQVTVEVPASSANLGPGFDAVGVGLDIRDSCTVAV
                     REGRGVAVVVTGEGEGTVPTDDHHLVVRAMRRAWEVLGVAGPPSLHITCGNAVPHGRG
                     LGSSATAIVTGISAAYGLWTLAHEGDGRLDLDVINDLAADLEGHPDNSSASVFGGMTL
                     SWLDDRTGRDGIPHTRTVRLTPHPSIRPHVLVPDAQLSTATARAVLPSQIRLADAAAN
                     SARVGLLVHAMTADPALLLPATRDYLHQEPRRPSYAASMALVDALRAAGVAATISGAG
                     PSVLALAIDAEQAALVTSSAPARWRVVAPGFADTGARLVAAAGG"
     gene            7590..9689
                     /gene="rho"
                     /locus_tag="PKU75_10425"
     CDS             7590..9689
                     /gene="rho"
                     /locus_tag="PKU75_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPF81337.1"
                     /translation="MTDTTTLEPAAAAGERPRRSGALTAMRLAELQGLASSMGITGTS
                     KMRKGDLIDVIRAKQSGTSLASADTTGAHSTGTQSTGTQSHSDQSTGIVSTGTQTTTR
                     RRAGRPSGEPKAADSAPEPASEENRLPAEATDAPAEARREERGTRRDRRERQQDRGAE
                     SRQGESRQPLDRQSTDRQSTDRQSEDRHVDDRQGKDWQGEDRQNRQAREAQQRDGRDR
                     DSQGRDDRDRDSQGRDGQQRDNRDGSRDNGNRDNDNRQNQGGQQNRYDDEDGQGSRRR
                     GRNRGRNRDKRRGNRPEGTDFDGEPQLSEDDNIVPVAGILDILDNYAFVRTSGYLPGP
                     NDVYVPLGMVKRHGLRKGDAVTGAVKTPRDGEEGGQTVQAGRNQRQKYNPLVRLDTVN
                     GMTTEQAKLRPEFGKLVPLYPQERLRLETEPGILTTRVIDLVAPIGKGQRGLLVAPAK
                     AGKTLILQAIANAITTNNPETHLMVVLVDERPEEVTDMQRAVKGEVIASTFDRPADDH
                     TTVAELAIERAKRLVEMGHDVVVLLDSITRLGRAYNLAAPASGRILSGGVDSAALYPP
                     KRFFGAARNIENGGSLTILATALVETGSKMDEVIFEEFKGTGNMELKLDRQLANRRIF
                     PAVDVNNSGTRREEILLAPEELKIMWKLRRVLSALDPQQGIELLLDRLKKTKSNYEFL
                     IQVQQTSSIRLDDDEQA"
     gene            complement(9718..10389)
                     /locus_tag="PKU75_10430"
     CDS             complement(9718..10389)
                     /locus_tag="PKU75_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81338.1"
                     /translation="MSEEPEETAAAEAEPTSAAEKPAKRRGPGRPPSPKQLARALRGR
                     EKTDAEAAAPDAPTGPVIRKAKSRDLAAVVALRALMYAAMGASPERVSDAAWQANALR
                     WLQVNLGNRDVHVAVADVNGSTVATAMGEVVERAPSPDNPTGRVGLLLNVATFPQYRM
                     TGLGQACVDAVMEWFRESTDVTSVEVFATTDGRRRYEAHGFAEHALPSLRLAIDRTPV
                     PADAD"
     gene            10579..10794
                     /gene="rpmE"
                     /locus_tag="PKU75_10435"
     CDS             10579..10794
                     /gene="rpmE"
                     /locus_tag="PKU75_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPF81339.1"
                     /translation="MKKDIHPDYVETTVTCTCGNTFTTRSTAKNGVIHADVCSNCHPF
                     YTGKQKILDTGGRVARFEARYGKKAAK"
     gene            10874..>11362
                     /locus_tag="PKU75_10440"
     CDS             10874..>11362
                     /locus_tag="PKU75_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HPF81340.1"
                     /translation="MLESAEALLAEHAELEAQLADPEVVGDQARYRDVGRRYAALSPV
                     VAAYHACQTAGEDLGAARELAAEDAAFAEEIPALEEAHQAAEEKLRRLLIPRDPDDDR
                     DVILEVKAGEGGEESALFAGDLLRMYLRYAEKRGWRTEVIDATESDLGGYKDVQVAVK
                     AKG"
BASE COUNT         1802 a         4098 c         3877 g         1585 t
ORIGIN      
        1 ggtgggttgg ttggtgcccc cggcaggatt cgaacctgcg ctcccgcctc cggagggcgg
       61 tgctctatcc cctgagctac gggggccgta tgccgtgcgc tgcggcacgt ggcgcgagca
      121 gtgaggctac cagcaccgga ctcccgaccg tgaatcggcc gggaatctgg tccggcccgg
      181 ccgtcggcca ggccaggacg ccggtgcagc catccggttg ggtggtgagt tgccggcact
      241 actacctcct gacgctgccg ctggcgccca ccaccgacca agcggtcatc gacgccttcc
      301 gagccgtcgc cgaagggggt cgccccagcg cgagcgacct gcagcgcgtg ccgcgctttc
      361 ctgccgagat catcgccggc tgggaccggt ggctggcagc ggacgcaggt ggcgctcgcc
      421 tggccggcgc ccccgtgaag ctagcccgga tcgagggggg tgacgggccg ctcgccctga
      481 cgatcgagtt cagcatccac gacgacgcca acgccgacgg cggctgggtg ttcgagtact
      541 ggctctacac cgtgtgtgcc ccgcccgtcc cggggacccg gcaggtcgtc ggcatccggc
      601 aggccgatcg gtgggacgag cccgaactga tctcgctcgg tgacgacggg gtccgcgtgg
      661 gggagcgttt cctgtccggc ccgacctgcg agccgccctg gccggacacc tcgacgcgtc
      721 cgcgccgtga cagtgccgtc ccaccggggc gcggtggaat ggtggcgcac gggcccacgc
      781 gcgctccgta gaatcgcccg gtgacccccg aacagctggc cgccgcgatc cgcgctgccc
      841 tcgccgacct cgtcgccgcc ggcgagttcg atctcgaggt gcccgagacc ctccgggtcg
      901 agcgtccgcg gcaggccggg cacggggact ggtccaccaa tgtcgcgatg cagctggcca
      961 agacggccgg tatgccgccg cgggacttcg ccggcaagct cgccggcgcg ctggagcagg
     1021 tcgcggggat cgccgccgtc gaggttgccg ggcccgggtt catcaatatc accctggatg
     1081 cggcgagcgc cggcgaactc gcgcgcacca tcgtggaggc gggcgcggcg tacggtcagg
     1141 gcacgagcga agccggccgc gtcgtcaacc tcgagtacat ctcggcaaac cccaccggtc
     1201 cactgcacat cggccacacc aggtgggcgg cgctcggcga cgcgatggtc cggctgttgc
     1261 gcgcggccgg cgccgaggtc gcggccgagt actacatcaa cgacgccggc ggccaggtgg
     1321 ccaagctcgg cgggtcggtt ctcgcgaagc tgcgcggcga ggcggcgccc gagggcggct
     1381 acgacggaga gtatgtcgac gggctggccg agcaggttcg cgcggggcgc cccgacatcc
     1441 tcgacctgcc ggccgacgag gcgctgcagg ccgcctggga tgtcgcctac ccgatccagc
     1501 tcgcggcgat caagcagacc ctggccgatt tcaatgtcgc cttcgacgtg tggttctccg
     1561 agaagagcct gcacgacgcc ggcgccgtcg agcaggcggt cgcgcggctg cgtgagcagg
     1621 ggcacgtctt cgaccaggac ggtgcgatct ggttgcgcac caccgatttc accgacgaca
     1681 aggaccgggt gctgatccgg gccaacggcg tgccgaccta tttcgccgcc gacgccgcct
     1741 actacctgag caagaaggac cggggcttcg acgagaagat ctatctcctc ggcgccgacc
     1801 accacggcta tatcggccgg ctcaaggcga tcgccgcctg cgccggcgac gatcccgagc
     1861 acaatatcga ggtcaagata ggccagctgg tcaatatcgc cggcgagcgc atgggcaagc
     1921 gacgcggcaa cgccctctat atggacgagc tgatcgaatg gatcggcccg gatgcgctgc
     1981 gctactcgct cgcccgctac ccctccgaca gcccgctctc gctcgacggg gaggagatgc
     2041 gccggcagac caacgacaat cccgtcttct acgtccagta cgcgcacgcg cgcacctcca
     2101 atgtcgcccg tctggccgcc gaggacggcg tacatcggga ggacggcttc gacccgtcgc
     2161 tgctcaccga cccgaccgaa gcggcgctac tcgcggtgct cggcgacttc ccgcgtgtca
     2221 ccgcgcaggc cgcgtcgttg ctcgaaccgc accgagtcgc gcgctacctg gaaaacctcg
     2281 ccgcccactt ccacaagtgg tacgacacct gccgcgtgcg gccgatgaat gccgacgaac
     2341 ccgtcaccga cctgcaccgg acgcgcctgt ggctcaacga tgccacgcgc acagtgttgg
     2401 cgaacggcct tgggctgctt ggcgtttcgg cgcccgagag gatgtagctc gtgcgcgcgc
     2461 atgaggcggg agtcctgcac gcggacggct atggcggccc gccgatctat ctgcccttcc
     2521 ccgatgacgt catggccctc acgccggcgc tgtggagccg gactgtgcga cgccgggacg
     2581 acggcgtgct cgaagtcggg ggcgtcagcg tcctggacct ggcgcgggag cacggcaccg
     2641 cggcatacat cctggacgag gccgacttcc gggcccgggc aggcgcattc cgcgacgaat
     2701 atgccgccgc cttcgcccat ctcggcggcg ccgacgtcta ttacgccggc aaagcgttcc
     2761 tctgctcggc cgtcgcacgg tgggtcatgg aggagggtct gaacctcgac gtctgcacgg
     2821 gcggagaact ggcggtcgcg cagcgggtcg ggttctccgg cgagcggatc gccctgcacg
     2881 gcaacaacaa aaccctggca gagatccgcc aggcgttgag ctatggcgtg ggccggatcg
     2941 tcgtcgactc cttcgtcgag atcgaacgga tcgtcgcggt cgccacggaa ctcggggtcg
     3001 tcgcgccggt catgctgcgc gtcacggtcg gtgtcgaggc gcacacgcat gagttcatcg
     3061 ccaccggcca cgaggaccag aagttcgggt tcagcctcgc gggcgacgtc gccttcaacg
     3121 gcgtccgcga gatcctcgcg caccccggtc aactgcgctt cctcggcctg cacagccaca
     3181 tcggcagtca gatcttcgac acggccgggt tcgagacggc cgcccggcgg ctggtgcggc
     3241 tccaccatcg catcgccgac gaactgggct gtgccgcacc ggaaatcgac ctcggtggcg
     3301 gctatgggat tgcctatacc agccagcaca ccccgctttc gacggccgtg ctcgccgagg
     3361 agatgtccca catcgtcgag caggccttca agaccgtcgg cgacgggtcg ctcgacgatg
     3421 tgccccgggt cagcatcgag ccgggacgag cgatcgtggg cccggcgatg ttcaccctgt
     3481 atgaagtggg caccgtcaag ccggtcacgg tcgcggacgg tcacgtgcgc acctatgtat
     3541 ccgtcgacgg cgggatgagc gacaacgccc gccccgcgct gtacgacgcg gacttctccg
     3601 tcaccctcgc cagccgcgca tcctctgccg cgccgctggt cgtgcgcgtc gtcggccgcc
     3661 actgcgaggc gggggacatc gtcgtgcagg acgaatggct gccggccgac gtgacacccg
     3721 gcgacctgct cgccgtgccg ggcaccggcg cctactgccg gtcgctgtcg agtcagtaca
     3781 accacacgcc ccggccgccg gtcatcgcgg tgcgcaacgg tgagtcgcgc gtcctgatcc
     3841 gccgggaaac catcgacgat ctgctgggac tggatgtggg atgagcatga gtgaacacac
     3901 aaaccctctg ggcgacaagc cgatccgagt cgcgctgctc ggctgtggcg tcgtcggctc
     3961 ctccgtggcg cgcatgctcg tcgaaagcgc cgacgatctg cgcgcccgca tcggcgcgcc
     4021 cgtcgaactc gtggggatcg ccgtgcgccg ggtcggcaag gaccgcagcg atgtcccggt
     4081 cgaccctgca ctcttcacga ccgatgccga ggacctcgtg acgcgcgccg acatcgtcgt
     4141 cgaggtcgtc ggtggcatcg atccggcgcg cgggctgctg ctgaaagcga tggagagcgg
     4201 cgcctccgtg gtgaccgcca acaaggctct gctcgccgag gacggcccga ccctctatgc
     4261 cgccgccgac gcggccggcg tcgacctcta ctacgaagcc gccgtggccg gtgccatccc
     4321 gatcctgcgt cccatgcgcg aatcgctcgc cggtgacgat gtcaccaaag tgctcgggat
     4381 cgtcaatggc acaacgaatt tcgtgctcga caagatggac accaccggcg cgggattcga
     4441 ggagacggtg gcgcaggcac aggcgctggg ctatgccgag gccgacccga ccgccgacgt
     4501 cgaaggcttc gacgccgccg ccaaggcagc cctgctcgcc tccctcgcct tccacacgcg
     4561 cgtcaagggc gctgacgtct tccgcgaggg catcagcgat gtcacggccg ccgatatcgc
     4621 cgccgccaag gccatggacc atgtcgtcaa gctgctcgcg atctgcgaac gcacgaccgg
     4681 ccagaacggc acgagcggtg tcagcgtccg ggtgcacccc gcgatgatcg cgcgcactca
     4741 tccgctggcg tcggtccgcg acgcctacaa cgcggtcttc gtcgaggcct cggccgcggg
     4801 acagttgatg ttctacggcc gtggcgccgg cggcgacccg accgcctcgg ccgtcctggg
     4861 cgacctcgtc gcggtggccc ggcacatcgt cgcgggcggg cgcggaccgg gggagtcggc
     4921 atacgccgat ctgcccatcc tcgacgtcgg cgactccgtg acccgctatc acatcagcct
     4981 cgacgtggag gaccggccgg gcgtgctcgc gcaggtggct caggcgttcg ccgacaccgg
     5041 cgtgtcgatt gagactgtgc gccaacacat tcggaccaac tccagctccg gcgaggcccg
     5101 agccaatctc gtcatcgtca cccatgcggc gaccgaagcg gccctggccg ccacggtcga
     5161 cgccctggcg gccagtgagg tcgtcgccga agtcgtctct gtgatgcgtg tggaaggagc
     5221 atgatcccca tggcccacca gtggcgtggc gtgatgcgcg agtatgccga tcggttgccg
     5281 atgctggacg gggcgccggt catcaccctg cacgagggag ggacccccct catcgccgcc
     5341 gagaagctct cggatcgcgt cggggcgcgc gtcttcgtca agtacgaagg actgaacccc
     5401 accggttcct tcaaggaccg cggcatgacg acggccatct ccatggccgc caagcacggc
     5461 gccaaggcgg tcatctgcgc gagcaccggc aacacgagcg cgagcgccgc ggcatacgcc
     5521 acgaaggccg gtatgacgtg tgccgtcctc gtgcccgacg gcaagatcgc tatgggcaaa
     5581 ctgagccagg ccatcgcgca cggcgcgacg ctgctgcagg tcgagggcaa cttcgacgac
     5641 tgcctcaccg tggcccgcaa gctcgccgag gtctatccgg tcgacctcgt caactcggtc
     5701 aacccggcac gcatcgaggg ccagaagacc gccagcttcg agatcgtcga cgcgctcggg
     5761 gacgccccgg acatccattg cctgcccgtc ggcaacgccg gcaacatcac ggcatattgg
     5821 aagggttatc gcgaatacgt tgcgggacac ggcattcccg acgtgccgga cggcccggcg
     5881 acgaaacgac cgcagatgtg gggcttccag gcagccgggt ccgcgcccat cgtgctgggc
     5941 caccccgtcg accagcccga gaccatcgcg accgcgatcc ggatcggcaa ccccgcctcg
     6001 tggcagcagg ccgaagaggc ccgcgacgac tccggcggac gcatcgaggc ggtcaccgac
     6061 cagcagatcc tcgaggcgca ccggctgctg tcgagcaccg aaggcatctt cgtcgaaccc
     6121 gcctcggcgg caagcgtcgc gggcctgttg caggtgcacg cggccggcgg cgtccccgag
     6181 ggcgcgacga tcgtgtgcac cgtcaccggc cacggactga aggatcctga ctgggcgctc
     6241 aaggggctcg acggtgaacc cgttgcgccg caacgcatcc cggtggatgt ctatggcgcg
     6301 gcggtcgcgc tcggcctcga cggctgacga tgcccgggga gatgccgggg acgacctggc
     6361 gcgcaggaca ccaggtcacg gtggaggtgc ccgcgagcag cgccaacctc gggcccggtt
     6421 tcgatgccgt cggcgtcggc ctggacatcc gcgactcctg caccgtcgcc gtccgcgagg
     6481 ggaggggtgt cgccgtcgtc gtcacgggcg agggcgaggg aaccgtgccc accgacgacc
     6541 accacctcgt cgtgcgcgcc atgcgtcgcg cgtgggaggt cctcggcgtc gcggggccgc
     6601 cgagcctgca catcacgtgc ggcaacgcgg tgccgcacgg ccgcggactg ggatcctcgg
     6661 cgacggccat cgtcacgggc atcagtgcgg catacggcct ttggacgctc gcacacgagg
     6721 gcgacggccg gctcgacctc gacgtcatca acgacctcgc ggccgatctc gaaggccacc
     6781 ccgacaactc gtccgcgagt gtgttcggcg ggatgaccct gagctggctc gacgaccgaa
     6841 ccgggcggga cggcatcccg catacgcgca cggtgcgcct gaccccgcac ccgagcatcc
     6901 ggccgcacgt gctcgtgccc gacgcgcagc tgagcactgc gaccgcccga gcggtcctgc
     6961 ccagccagat ccgcctggcc gatgccgccg ccaactcggc ccgtgtcggg ctcctggtgc
     7021 acgcgatgac cgccgacccg gcgctgctgc tccctgcaac gcgcgactac ctgcaccagg
     7081 agccgcgccg gccgtcgtat gccgcgtcca tggccctggt ggatgcgctg cgcgcggccg
     7141 gggtggctgc caccatctcg ggcgccggtc cctcggtgct cgcgctcgct atcgacgcgg
     7201 agcaggccgc gctcgtgacc tcgagcgcac cggcgcgctg gcgcgtcgtg gcgccggggt
     7261 tcgcggacac cggggcgcgg ctggtggcgg cggccggagg ctagatcacg ttatgggaat
     7321 agcggtcggc cgagtgtgcg ttattcggac atcgcggtgt acagtgacac cgcaagcgca
     7381 gggggcagtc ggctgaagat cgccgcgccc accgcgacct gcaaaatcac tctctgccct
     7441 ttcgttgcgt tgctctccag ggtccccccg aggctgcggc actaggcgga gaactcctca
     7501 gctgtcccgc caattcgacg gggccgcaac gtaatccagg tcgtatcccc tcatctgggg
     7561 atgggcctca tccgaggaaa gggtccttcg tgaccgacac caccactctc gaacccgcgg
     7621 ctgcagcggg cgagaggccg cgccgttccg gcgccttgac cgccatgcgc ctcgccgagt
     7681 tgcaggggct ggccagcagc atggggatca cgggcacctc caagatgcgc aagggcgacc
     7741 tgatcgacgt gatccgggcc aagcagtccg gcacctcgct cgccagcgcg gacaccaccg
     7801 gcgcccactc cactggcact cagtccactg gcactcagtc ccatagcgac cagtccactg
     7861 gcattgtctc gactggcacc cagaccacca cccgccgccg cgccggccgc ccctcgggcg
     7921 agccgaaggc cgccgacagc gcccccgagc ccgccagtga ggagaaccgc ctgccggccg
     7981 aggccacgga cgcgccggcc gaggcccgcc gcgaggagcg gggcacgcgc cgtgatcgcc
     8041 gcgagcgtca gcaggatcgc ggtgccgaga gccgccaggg cgagtcccgc cagcctctcg
     8101 accgccagtc caccgaccgc cagtccaccg accgccagtc cgaagaccgg cacgtcgacg
     8161 atcggcaggg caaggactgg cagggcgagg accggcagaa ccgccaggcg cgcgaggccc
     8221 agcagcgcga cggccgcgac cgcgacagcc agggccgcga cgaccgcgac cgtgacagcc
     8281 agggccgcga tggccagcag cgcgacaacc gggacggcag ccgcgacaac ggcaaccgcg
     8341 acaacgacaa ccgtcagaac cagggcggcc agcagaaccg ttacgacgac gaagacggcc
     8401 agggcagtcg tcgtcgcggc cgcaaccggg gccgcaaccg cgacaagcgc cgaggcaacc
     8461 gccccgaggg caccgacttc gacggcgagc cgcagctctc cgaggacgac aacatcgtcc
     8521 cggtcgccgg catcctcgac atcctcgaca actacgcgtt cgtgcggacc agcggctacc
     8581 tgcccgggcc caacgacgtc tacgttccgc tcggcatggt caagcgtcac ggcctgcgca
     8641 agggcgacgc cgtcaccggc gcggtgaaga ctccgcgcga cggcgaggag ggcggccaga
     8701 ccgtccaggc cggccgcaac cagcgccaga agtacaaccc gctcgtgcgc ctcgacaccg
     8761 tcaacggcat gaccaccgag caggccaagc tgcgcccgga gttcggcaag ctggtcccgc
     8821 tctacccgca ggagcgcctg cggttggaaa ccgagccggg catcctgacc acgcgcgtca
     8881 tcgacctggt cgccccgatc ggcaagggcc aacgcggtct gctcgtcgcc ccggccaagg
     8941 ccggcaagac gctcatcctg caggccatcg ccaacgcgat caccaccaac aaccccgaga
     9001 cccacctgat ggtcgtgctc gtcgacgagc gcccggaaga ggtcaccgac atgcagcggg
     9061 ccgtcaaggg tgaggtcatc gcctccacct tcgaccgtcc ggccgacgac cacaccaccg
     9121 tcgccgagct ggcgatcgaa cgggccaagc gactggtcga gatgggccac gacgtcgtcg
     9181 tgctcctcga ctccatcacc cgtctcggcc gggcctacaa cctcgccgcc ccggcgagcg
     9241 gccggatcct ctccggtggt gtcgactccg ccgcgctcta tccgcccaag cgcttcttcg
     9301 gcgccgcccg caatatcgag aacggtggct cgctgacgat cctcgcgacg gcgctcgtcg
     9361 agaccgggtc caagatggac gaggtcatct tcgaagagtt caagggcacc ggcaatatgg
     9421 agctcaagct cgaccggcag ctggccaacc gccgcatctt ccctgcggtc gacgtcaaca
     9481 actccggcac gcgccgcgag gagatcctcc tcgcgccgga ggagttgaag atcatgtgga
     9541 agctgcgccg ggtgctctcc gccctcgacc cgcagcaggg catcgaactg ctcctggacc
     9601 ggctcaagaa gaccaagagc aactacgagt tcctcatcca ggtccagcag acgagttcga
     9661 tccgcctcga cgacgacgaa caggcctgag tccgcgcgtt tcggtatgcc gcgcggttca
     9721 gtccgcgtcg gcgggcaccg gggtgcggtc gatcgccagc cgcaggctgg gcagggcgtg
     9781 ttcggcgaac ccgtgcgcct cataccgccg gcgcccgtcg gtcgtggcga acacctcgac
     9841 cgatgtgaca tcggtgctct cgcggaacca ctccatcacc gcatcgacgc aggcctgtcc
     9901 caggccggtc atgcggtatt gcgggaaggt cgcgacgttc aggagcagcc cgactcgtcc
     9961 cgtggggttg tccggggacg gcgcccgctc gaccacctcg cccattgccg tcgccacggt
    10021 cgacccgttg acgtcggcca cggcgacgtg gacatcacgg ttgccgaggt tgacctgcag
    10081 ccagcgcagc gcgttggcct gccacgccgc gtccgagacc cgttcgggac tggcgcccat
    10141 ggcggcatac atcagcgcgc gcagcgcgac gacggcggcg aggtcgcggc tcttggcctt
    10201 gcggatgacc ggcccggtgg gcgcatcggg cgcggccgcc tcggcatcgg tcttctcccg
    10261 cccccgcagg gcgcgggcca gctgcttggg cgagggcggg cggccggggc cgcgccgctt
    10321 cgccggcttc tcggccgcgc tcgtcggctc cgcctccgcg gcggccgtct cctccggctc
    10381 ctcgctcacg ggtgccagta tgccgggcct ggcgcgtcag ggaatgtggc cgggtcgccg
    10441 cgcgtttggg tgagggcaac cctctctggc acaattgaga ggttgggccg gttcacgaca
    10501 ggcgccgccg atggcgcagg gcttgcactt tgcgacaagt gggctcgtcg acccggccga
    10561 tctgcttaag gagcagcgtt gaagaaggac atccaccccg actacgtgga gaccacggtg
    10621 acctgcacct gtggcaacac gttcaccacg cgcagcacgg ccaagaacgg cgtcatccac
    10681 gccgacgtct gcagcaactg ccacccgttc tacacgggca agcagaagat cctcgacacc
    10741 ggtggccgcg tggcccgctt cgaggcccgc tacggcaaga aggccgccaa gtagctttcc
    10801 cggcgcgccg gtccaccctc gtggtgggcc ggcgcgttcg cttttgccac ggcataccga
    10861 aaaggaagtc ctgatgctcg agtccgccga ggccctgctc gccgagcacg ccgagttgga
    10921 ggcccagctc gccgacccgg aggtggttgg cgaccaggcg cgctatcgcg atgtcggccg
    10981 gcggtatgcc gcgttgagcc ccgtcgtcgc ggcataccac gcgtgccaga cggcgggcga
    11041 ggacctgggc gcggcccgcg agctcgccgc cgaggacgcc gcgttcgccg aggagatccc
    11101 ggccctggag gaggcgcacc aggccgccga ggagaagctg cgccggctcc tcataccgcg
    11161 cgatcccgac gacgaccgcg atgtcatcct ggaggtcaag gcgggggagg gcggcgagga
    11221 gtcggccctg ttcgccggcg acctgctgcg catgtacctg cggtatgccg agaagcgcgg
    11281 ctggcgcacg gaggtcatcg atgcgacgga gtccgacctc ggcggctaca aggatgtcca
    11341 ggtggcggtc aaggccaagg gc
//
LOCUS       DAOPJG010000107        11199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6092_length_11199_cov_7.229899, whole genome shotgun sequence.
ACCESSION   DAOPJG010000107 DAOPJG010000000
VERSION     DAOPJG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11199
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6092_length_11199_cov_7.229899"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKU75_10445"
     CDS             complement(<1..205)
                     /locus_tag="PKU75_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HPF81341.1"
                     /translation="MQHVGTTEGLPDGLVAALGAAQEESAANTGMHVNLAVGYGGRRE
                     IADAMRSIVRHHGSGGGTIDDLAD"
     gene            complement(474..2516)
                     /locus_tag="PKU75_10450"
     CDS             complement(474..2516)
                     /locus_tag="PKU75_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81342.1"
                     /translation="MHEAYLTMSGIAALAGVQRPVVSMWRKRPRARGRVVPFPKPADY
                     VDGVERFSAREIVEWLELSGRGTNPEARDDALAHSAFPDFGIPATTAGEGAEGLLALK
                     ARTGAQLTSLGLEELLDLSDAEDPDDEAIYSEILALGPTCPAVAAYVDELVDAAYDTA
                     TAAEVLEALLRSPLERPEFLADKATNFMGAVTAALARELPDRSVLLVDPDGRARTLIA
                     AAAGHLEESRDYAADVRGDGKSSRRTRRRLICRGVSLGPLSADQGVVGIINAVPCGGS
                     ALELLAHIEDVCLDFTVAQPVVVIGPATVLCDELSEPESEQLRAVFLRGRLRCAVRLP
                     HGMSPGRPRERVALWVLGAPQDSLLVGQRWFGAGDLTDDALGDAEVDAIATDVVACTR
                     ARLARAHAFTSLRLMRTSDVLAEDAGLVPLAVSPERHLQPSAAERVVTIRQAFARLAG
                     DIHDCQVARVRVDPSETSSSPPTLLGELVDRGVMRVHRGTRIDIDGLRADGAVSVFTT
                     DDVLAHRYPPAMGLGALEFVTDHPRALRTEPGDVVFVSAPAPAAVVDRDGGNAVCSPV
                     RILRVPATSGLSTHGLAAVINGPRVRGRDWRAWPVPVPDPAAAPGLDEALRLLEEERA
                     WHARRASDLARLAAELAGAAGHVQASVTVLSESHKVTENVTISSLERGDRSREQPA"
     gene            2673..4115
                     /locus_tag="PKU75_10455"
     CDS             2673..4115
                     /locus_tag="PKU75_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81343.1"
                     /translation="MTNNSTPNVTFDTNLRAVQITNLTLIDPAVTTEALRWSTGVRGP
                     ALAPAEMAGADLSVFAERAFDLGARALAQAGNGQDVIHIQSLVDSVEKRTAQTVKEAA
                     DATAGVVNQAATSMREAAATAKKELISEEQRARKAFHESVASAQAALTTEVRNLLGGE
                     DPELVARLALVVERFKTELAERSERHSTDLYEKATKVLDPDDPASPLAKHRAISEKQH
                     QVLTQQMEKQTSKLESGLRELRAAVLVQHEITDAKNKLAHVTPLKGGSYEDSVNAILA
                     EIAVGLGDSYEATGRIAGALSARNLKGDGVLTIQGGPARLVIEMCTSERKAWNDYLDE
                     AERNRSAQASLGVVPTADLNAGHGLRVLGPRRLVMAFEAAQDDPDILRTAVLFLRLSA
                     LAAANAGKGGRLDTAREAIGKALDALGKIEAIRKASGTIRTNADTIDKSALALHSSLS
                     RLLDEARIALDDNAVEAIGDAPANGPTQVA"
     gene            complement(4123..5157)
                     /locus_tag="PKU75_10460"
     CDS             complement(4123..5157)
                     /locus_tag="PKU75_10460"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPF81344.1"
                     /translation="MRVGVFGATGQVGGVMRTLLAERGFPVEQIRYFASARSAGSTLP
                     WGDGEVTVEDTATADFSGLDVALFSNGGSTSKEWAPKVAAAGALVIDNSSAWRKDPDV
                     PLVVSEVNADDLLNIPKGIVANPNCTTMAFMPVLKPLHDKASLVRLHVSSYQAVSGSG
                     GKGVQELDRQLKGGYAAGDPTGLALDGRAVAVPEPQVYAVPVGFNVVPLAGSLVDDGS
                     GETDEEQKLRHESRKILHIPDLRVSGTCVRVPVFSGHSLAIHAEFADDIAPDEALDML
                     KNAPGVVVTDVPNPLEAAGRDEVFVGRVRADQAAPEGKGLNLFVVGDNLRKGAALNAV
                     QIAEEWLKRR"
     gene            complement(5221..5730)
                     /locus_tag="PKU75_10465"
     CDS             complement(5221..5730)
                     /locus_tag="PKU75_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HPF81345.1"
                     /translation="MTIVRPRWATLGAVLLAQAGLVVAAVAPQLSARVRGEEIRLAVT
                     VVDPIDPFRGAYVDLDYPGLRTLRDPSASQPDPGVAPGERGTAYVLLRRDGDVWVQDG
                     AQRERPAGGTYLACDDGDWRLRCGIESWFLPQGRAASLGRALADGDAVAVVRVDGRGN
                     AALVRIEAG"
     gene            complement(5727..6920)
                     /locus_tag="PKU75_10470"
     CDS             complement(5727..6920)
                     /locus_tag="PKU75_10470"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HPF81346.1"
                     /translation="MAAPTGVKHPVNPEQWNWLVEQLRDWEREGLVSPDAAGAIRGRY
                     VEGPPSRGRSAWDVLLWLGGAFVVVGLIWLVASNLDQLSPLWRFVLLAAIWVGLSVGA
                     ERLDEPFRSVGRLLAAGAFGAVIFQAAQSVQVPAYEPRLLAAWGIGAALYAYAVRSRP
                     ALIVGLGALTAWYAWQAGESADGVFGVVVALMIGGLVAACAALLQPGPWASFAVVWRI
                     VAALVSLGGIFAAALPIHDRHGTWPVIATIGAAVAVLAVVAAAVRARSRTDRIELAAA
                     AAVALAGAGLAAWRPPVDLLLDTGNPTPAMWVRTSVSVLVFLIAAGWYAVLAQWRSSP
                     ALGALALAALVVFTTFQSWAVFAPIISGAALFLIVGLVLLGTGYLAERARRRLRMTEP
                     EGEPS"
     gene            complement(6969..7895)
                     /gene="era"
                     /locus_tag="PKU75_10475"
     CDS             complement(6969..7895)
                     /gene="era"
                     /locus_tag="PKU75_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPF81347.1"
                     /translation="MRPSEQPYVAGFACLVGRPNAGKSTLTNALVGDKVAITSSKPQT
                     TRHTIRGIVTGPASQLILVDTPGLHKARTLLGERLNDLVRATLLDVDVVGFCLPADQR
                     IGPGDRFIANELTELMRGKKLPVVAIATKIDTVDKKRLAEHLIAIDQLADWAAIVPCS
                     AARGDQVDDVLKVLTAYLPPSPGPLYPEGVVTDEETDTRIAELVREAALEGVRDELPH
                     SIAVVVEEMTEREDAPADKPILDIQVNIFVERDSQKAIIIGKGGSRLREVGTEARAGI
                     EKLLGRRVYLHLRVKVAKDWQRDPRQLQRLGF"
     gene            complement(7892..9223)
                     /locus_tag="PKU75_10480"
     CDS             complement(7892..9223)
                     /locus_tag="PKU75_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPF81348.1"
                     /translation="MPTRLILVAIAAILFAFVLALGEAAISRSSRVRAEELGDEGRAG
                     ARSLAKILGDSAAYLSVTAFLRAVAEAVAAVCITLSVSSLTDSFWRTLLISIGIMSLV
                     SFVAVGVSPRTLGRQHSDRVALALAPLIAWLHRILGPVARLLVLIANMVTPGRGYRDG
                     PFQSESELRDLVDIAGENSLIEDDEREMLHSVFELGDTVAREVMVPRTDMVSIEVGKH
                     GRQAMSLFNRSGFSRLPVVGDGPDDVVGLVYLKDLVRHMTSGGVELSTLPIASIMRPM
                     PFVPESKPVDDLLREMQLGSVHIAIVVDEYGGTAGLVTIEDIIEEIVGEIDDEYDRET
                     PGVEDLGDGRFRIPATMNVDDFAEEFDIDLDEDDVDSVGGLIAKQLGQVPILGSTCEV
                     GRFLLTAERMAGRRHRIASVLVTPLPPVVGADGELVEAQDAAGGRSREQRA"
     gene            complement(9226..9693)
                     /gene="ybeY"
                     /locus_tag="PKU75_10485"
     CDS             complement(9226..9693)
                     /gene="ybeY"
                     /locus_tag="PKU75_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPF81349.1"
                     /translation="MSVDVLNETDYVVDEVELVDLSRHVMAAMRVHPQAELCVRLVDE
                     ATMTVLHEQWMDLAGPTDVMSFPMDELRPGREGEAAEEGVLGDIVLCPSVATKQAAEA
                     GHAPAEEMLLLTTHGILHLLGFDHAEPAEEKEMFDLQRQLLLTFLASRGRPAG"
     gene            complement(9671..10723)
                     /locus_tag="PKU75_10490"
     CDS             complement(9671..10723)
                     /locus_tag="PKU75_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPF81350.1"
                     /translation="MSDHPSQASGLPQPVHPMHTVEIPSSVAMVSLLGPRDELLRTLE
                     RQFPAVEILVRGNEFRMSGPSADVAIVERTIDELLAVIAGGQPLNRDAVERSIGMLRA
                     QTTERPADVLTMNIVSNRGRTIRPKTLNQKRYVDAIDAHTIVFGIGPAGTGKTYLAMA
                     KAVAALQAKQVNRIILTRPAVEAGERLGFLPGTLTDKIDPYLRPLYDALHDMIDPESI
                     PRLMAAGTIEVAPLAFMRGRSLNDAFVILDEAQNTSPEQMKMFLTRLGFGSKMVVTGD
                     VTQVDLPGGTQSGLRVVQEILQGIEDVHFSQLTSQDVVRHRLVGEIVDAYGRWDTKTA
                     HAPTTRKAAGERRRPQ"
     gene            complement(10749..11090)
                     /locus_tag="PKU75_10495"
     CDS             complement(10749..11090)
                     /locus_tag="PKU75_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPF81351.1"
                     /translation="MSEDCLFCKFIARDIEPDVVAESELALAFRDINPQAPTHVLVIP
                     KKHVATAAELGEHPGDLAAVVALAAQVAQDEGLGAGYRLVTNTGADAGQSVFHCHFHL
                     LGGRTMAWPPG"
BASE COUNT         1865 a         3813 c         3870 g         1651 t
ORIGIN      
        1 tgtcggcgag gtcgtcgatc gtgccgccgc cgctgccgtg gtgacgcacg atgctgcgca
       61 tggcatccgc gatctcccgg cgcccgccgt agccgaccgc gaggttcacg tgcatgccgg
      121 tgttcgccgc gctctcctcc tgggcggcgc cgagcgcggc cacgaggccg tcgggcagac
      181 cctccgtcgt gccgacgtgc tgcacgcgcc aatcgtcggc gcgggcgatc tccgcggcga
      241 ggtcgccgat gatttcgact ccgggaggag caccccaggc tcggtattcg ggcgggaccg
      301 ggacccacaa gccccgggcg ggctgaaccc attcgttgcg acccgcaggg gcgtgcaacc
      361 ggcggcggac ctgatcggcc ggcacaccaa gaagtccggc taagtcatcc gtcgtcaacg
      421 ccgcgattcc gcgcccgagg gcccaatcgg ccgcatgcgg cgcactcacc cgttcacgca
      481 ggctgctcac gcgaccgatc acccctctct aaactgctta tcgtcacatt ttccgtgact
      541 ttatgagatt ccgagagtac cgtcaccgac gcttggacat gaccggcggc accggcaagt
      601 tctgcggcca gtcgcgcgag atcacttgct cgccgtgcgt gccacgcgcg ttcctcctcg
      661 aggagacgga gagcctcgtc gagcccgggc gcggcagccg gatccggcac ggggaccggc
      721 caggcgcgcc aatcccggcc acgcactcgc ggcccgttga tgaccgcagc taagccgtgg
      781 gtcgacaggc ccgatgtcgc cgggacgcgc agaatacgca ccggtgagca cacagcgttg
      841 ccgccgtctc ggtccacgac ggcggccggg gcgggcgcac tgacgaagac gacgtcgccc
      901 ggctccgtgc gcagcgcgcg aggatggtcg gtgacgaact ccagggcgcc caggcccatc
      961 gccggcgggt agcgatgcgc gagcacatcg tccgtcgtga agacgctcac cgcgccatcc
     1021 gcgcgcagcc cgtcgatgtc gattcgcgtc ccgcggtgga cacgcatgac gccacgatcc
     1081 acaagttcgc cgagcagcgt aggcggcgag gacgaagtct cggaggggtc gacgcggacc
     1141 ctggcgacct ggcaatcgtg gatgtcccct gccagacgcg caaacgcctg acggatcgtc
     1201 acgacgcgct cagccgccga gggctgaagg tgacgttcgg gcgagacagc gagcgggacc
     1261 agtcccgcgt cctcggccag cacgtcggag gtgcgcatca accgcagcga cgtgaacgcg
     1321 tgagcacggg caaggcgcgc ccgagtgcac gcgacgacat ccgtcgcgat ggcatcgacc
     1381 tcggcatcgc ccagcgcgtc gtctgtcaga tcaccggctc cgaaccaccg ctggccgacc
     1441 agcagggagt cctgaggcgc gccgaggacc cacaaggcta ctcgctcgcg cggtctccca
     1501 gggctcattc catggggcaa ccgcactgca caccggagcc tgccacgaag gaagaccgca
     1561 cgcagttgtt cggattcagg ttcggacaac tcgtcgcata gaacggttgc cgggccgatg
     1621 accacgacag gctgagcgac agtgaaatcc aggcagacat cctcgatatg ggctaacaac
     1681 tccaaagcgg agccgccgca gggcacggcg ttgatgatgc ccacgacccc ctgatctgcc
     1741 gagagagggc ccaggctcac cccgcgacag atcaggcgtc gccgggtcct ccgggagctc
     1801 ttgccgtcac cgcgtacatc agcggcgtaa tcgcgggatt cctccagatg accggcagcg
     1861 gcggcgatga gagtccgggc ccgcccatca gggtcgacga ggagcacgga ccgatcgggc
     1921 agttcgcgcg ccaacgccgc cgtcaccgcg cccatgaagt tggtcgcctt gtcagcgagg
     1981 aactccggac gttcgagagg tgacctgagc aacgcctcga ggacctcggc ggcggtcgcg
     2041 gtgtcgtatg ccgcgtccac gagttcgtct acgtaggcgg cgacggcagg gcaggtcgga
     2101 cccagcgcga ggatctccga atagatggct tcgtcatccg ggtcctcggc gtcggacagg
     2161 tccagcagtt cctccaaccc gagcgacgtc aattgcgcgc cggtgcgcgc ctttagcgca
     2221 aggaggccct cagcgccctc accggccgtt gtggccggaa tcccgaagtc cgggaaagcg
     2281 ctgtgcgcca aggcatcgtc acgcgcctcc gggttggtcc cgcgcccgct gagttcaagc
     2341 cactcgacga tctcgcgagc ggagaaccgc tcgacaccgt cgacataatc agccggcttc
     2401 gggaaaggga cgacgcggcc acgcgcccgt ggccgcttgc gccacatact gacgaccggc
     2461 cgctggacac ccgctagtgc ggcgataccc gacatcgtca ggtaggcctc gtgcacggat
     2521 ccgggtttgg tcatggtcaa gacagtagat gacttgggca caggtttctc gctagccact
     2581 gataaccctg gttatcagtt aagagttctt gttaaacgcc gagcaaagga cagagtggcg
     2641 gttgtccgga actaccgacc tgggaggtca tgatgaccaa caactccacg cccaatgtca
     2701 ccttcgacac caatctgcgg gccgtgcaaa tcacgaatct caccctcatc gaccccgccg
     2761 tgaccactga ggctctgcgg tggtccaccg gggtcagggg tccggccctg gccccggcgg
     2821 agatggccgg cgccgacctg tcggtattcg ccgagcgtgc cttcgacctg ggcgctcgtg
     2881 ctctagccca agctggcaac ggacaggacg tcatccacat ccagtccctc gtcgacagtg
     2941 tggaaaagcg aacggcacag acggtcaagg aggctgccga cgcaactgcc ggggtggtca
     3001 accaagccgc cacgtcgatg cgggaagccg ccgccacggc gaagaaggaa ctcatcagcg
     3061 aagaacagcg cgcccgcaag gcgttccacg agagcgtcgc ctcggctcaa gcagcgctga
     3121 caaccgaagt ccgcaacctg ctcggcggag aagacccgga gctcgtggct cgcctcgctc
     3181 tcgtggtgga gcggttcaag accgagttgg cggagcgctc cgagcgtcac tccaccgatc
     3241 tctatgagaa ggccaccaag gtgttggatc cggatgatcc cgcttcgccc ttggccaagc
     3301 accgggctat cagcgagaag cagcaccagg tgctgacgca gcagatggag aagcagacgt
     3361 cgaagctgga gagcgggctg cgtgagctgc gcgcggccgt tctcgtgcag cacgagatca
     3421 cggacgccaa gaacaagctc gcccatgtca ctccattgaa gggcggcagc tacgaggact
     3481 cggtgaacgc catcttggcc gagatcgccg tgggattggg cgattcttac gaagcgacgg
     3541 gtcgcatcgc tggggccctg tccgcacgca acttgaaggg cgacggcgtg ctcaccatcc
     3601 agggcggacc agcgcgcctg gtcatcgaga tgtgcacatc ggagcgcaag gcgtggaacg
     3661 actatctcga cgaggctgaa cgcaatcggt cggcccaggc ctcgctgggg gtggtgccca
     3721 cggctgatct gaacgctggg catgggctgc gcgtgctcgg cccgcgtcgg ctcgtgatgg
     3781 cattcgaggc cgcccaagac gatcccgaca ttctgcggac ggccgtcctc ttcctgcgac
     3841 tctccgccct cgctgccgcc aacgccggta agggaggccg cctggacact gcacgcgagg
     3901 cgatcggcaa ggcactcgac gccttgggca agatcgaagc catccggaag gcctctggca
     3961 cgatccgcac caacgccgac accattgaca agtcggccct ggccctccac tcatccctga
     4021 gcaggttgct ggatgaagct cggatcgcgt tggacgacaa cgccgttgag gctattggcg
     4081 acgcccccgc caacgggcct acgcaagtgg cgtgaccggc gcttagcgcc gcttgagcca
     4141 ctcctctgcg atctgcacgg cgttcagtgc cgcgcccttg cggagattgt cgccgacgac
     4201 gaacaggttg agacccttgc cctcgggggc ggcctgatcg gcgcgcaccc gaccgacgaa
     4261 gacctcgtcg cggccggcgg cctcgagtgg gttgggcaca tcggtgacga cgaccccggg
     4321 cgcgttcttg agcatgtcga gcgcctcgtc gggggcgatg tcgtcggcga actcggcgtg
     4381 gatggcgagc gagtggccgc tgaagaccgg cacccgcacg caggtgcccg agacgcgcag
     4441 gtcggggatg tgcaggatct tgcgggactc gtgccgcagc ttctgctcct cgtcggtctc
     4501 gccggacccg tcgtcgacga gcgacccggc gagcgggacg acgttgaagc cgaccggcac
     4561 ggcatacacc tggggttcgg ggacggcgac cgcccggccg tcgagagcga gcccggtggg
     4621 gtcgcccgcg gcatacccgc ccttgagttg acggtcgagt tcttggacgc ccttgccgcc
     4681 ggagccggag accgcctgat aggaggagac gtgcagacgc accagagatg ccttgtcgtg
     4741 caacggtttc agcaccggca tgaaggccat cgtcgtgcag ttggggttgg cgacgatgcc
     4801 cttggggatg ttcagcaggt cgtcggcgtt gacctcgctc acgacgagcg ggacgtccgg
     4861 gtccttgcgc caggcgctgg aattgtcgat gaccagcgcg ccggccgcgg cgaccttggg
     4921 cgcccactcc ttcgaggtcg atccgccgtt ggagaagagc gcgacatcga ggccggagaa
     4981 gtcagcggtc gcggtgtcct cgacggtgac ctcaccgtca ccccacggca gggtcgagcc
     5041 cgccgaccgc gccgacgcga agtagcggat ctgctcgacc gggaagccgc gctccgcgag
     5101 aagggtccgc atgaccccgc cgacctgacc cgttgcaccg aagactccaa cacgcatgcg
     5161 tccaaggata gggagggcgt ccagggatta tcggtcagtg accaccccgt gggcgcgccg
     5221 tcaccccgcc tcgatccgca cgagagcggc gttgccacga ccgtcgaccc tcaccacggc
     5281 gaccgcgtcg ccgtccgcca gcgcccgtcc gaggctcgcg gcccgcccct gcgggaggaa
     5341 ccagctctcg atcccgcaac gcaggcgcca gtctccgtcg tcgcacgcca ggtaggtccc
     5401 tccggcgggc cgctcccgct gggcaccgtc ctgcacccag acgtcaccgt cccggcgcag
     5461 caggacgtat gccgtgcccc tctccccggg cgccacgccc gggtcgggct gcgaggccga
     5521 tgggtcccgc agggtccgca agccgggata gtccagatcc acgtaggccc cccggaacgg
     5581 gtcgatgggg tcgacgaccg tgacagccag gcggatctcc tcgccgcgga cgcgggcgga
     5641 gagctgcggt gcaacggccg ccaccaccaa ccccgcctgg gcgagcagca ccgcgccgag
     5701 ggtggcccag cggggtcgga cgatggtcat gacggctctc cttccggctc ggtcatgcgc
     5761 agtcgtcgcc gggcgcgctc ggcgagatac ccagttccca gcagcaccag accgacgatg
     5821 aggaagagcg ccgctcccga gatgatcggc gcgaagaccg cccaggactg gaaggtcgtg
     5881 aagaccacca acgccgcgag agcgagcgca cccaacgccg gcgacgaacg ccactgggcc
     5941 agaaccgcat accacccggc ggcgatcagg aagacgagga cgctgacgct cgtccgcacc
     6001 cacatggccg gcgtggggtt cccggtgtcg aggagcagat cgacgggcgg acgccatgcc
     6061 gcgagcccgg cgccggcgag cgcgacagcc gccgcggcag ccagttcgat ccggtcggtg
     6121 cggctgcggg cgcgcaccgc cgcggccacc acggcgagca cggctacggc ggctccgatc
     6181 gtcgcgatca ccggccaggt gccgtgccgg tcgtggatgg gcagggcggc ggcgaagatc
     6241 ccgcccaggg acaccagggc ggcgacgatg cgccagacca cggcgaacga cgcccagggg
     6301 cccggctgca gcagtgcggc gcacgcggcg acgagcccgc cgatcatgag ggcgaccaca
     6361 acgccgaaga cgccgtccgc gctctccccg gcctgccagg cataccacgc ggtgagcgcg
     6421 ccgagcccga cgatcagggc gggccgggac cggacggcat acgcatagag cgcggcgccg
     6481 atcccccacg cggcaaggag cctcggctca taggcaggca cctgcacgct ctgtgcggcc
     6541 tggaagatca ccgctccgaa ggcccccgcc gccaggagcc ggccgacgct ccggaatggc
     6601 tcgtcgaggc gctcggcacc cacgctcagg cccacccaga tcgcggcgag cagcacgaac
     6661 cgccacagcg gggagagctg gtcgagattg ctcgccacca gccagatcaa cccgacgacc
     6721 acgaaggcgc caccgagcca cagcagcaca tcccatgctg agcggccgcg cgagggcggc
     6781 ccctcgacat acctcccccg gatcgcgccg gcggcgtcgg ggctgaccag cccctcccgc
     6841 tcccagtcgc gcaattgctc gaccagccag ttccactgct ccgggttgac cggatgcttg
     6901 actccagtcg gtgctgccat gccgccattg aagcgccggc gaccggcgcg cgtcatgagt
     6961 tgcgctactc agaagccaag tcgttgcaac tgcctggggt cgcgttgcca gtccttggcg
     7021 accttgaccc gcaggtgcag atagacccgg cggccgagca gcttctcgat gccggctcgg
     7081 gcctcggtgc cgacctcgcg caggcgcgag ccgcccttgc cgatgatgat ggccttctgg
     7141 gagtcgcgct cgacgaagat gttgacctgg atgtcgagaa tcggcttgtc ggccggggcg
     7201 tcctcgcgct cggtcatctc ctccacgacg acggcgatcg agtgcggcaa ctcgtcgcgg
     7261 acaccttcca gggcggcttc gcgcacgagt tcggcgatgc gggtgtcggt ctcctcgtcg
     7321 gtgacgacgc cctcggggta gagcgggccg ggcgacggcg ggaggtatgc cgtgagcacc
     7381 ttcagcacgt cgtccacctg gtccccgcgg gcggccgaac acgggacgat cgcggcccag
     7441 tccgcgagct ggtcgatggc gatcaggtgc tcggccaagc gcttcttgtc gaccgtgtcg
     7501 atcttggtcg cgatggcgac gacgggcagc ttcttgccgc gcatcagctc ggtgagttcg
     7561 ttcgcgatga accggtcgcc ggggccgatc cgctggtcgg ccggcaggca gaacccgacg
     7621 acgtcgacgt cgagcagcgt ggcccggacc aggtcgttga gtctctcccc caacagagtt
     7681 cgcgccttgt gcaggccggg ggtgtcgacg aggatcagct ggctcgcggg tccggtgacg
     7741 atgcctcgga tggtatgccg cgtggtctgg ggcttggaag aggttatcgc caccttgtcg
     7801 ccgacgaggg cgttggtcag cgtcgacttg ccggcgttgg gccggccgac caggcacgcg
     7861 aagccggcga catacggctg ctcggaaggc ctcatgcgcg ctgctccctg ctgcggccgc
     7921 cggccgcatc ctgcgcctcg acgagttcgc cgtcggcccc gaccacgggg ggcagcggcg
     7981 tgacgagcac cgaggcgatc cggtgacggc gcccggccat ccgctcggcg gtcagcagga
     8041 agcggcccac ctcgcacgtg gatccgagga tcggcacctg tccgagctgc ttggcgatca
     8101 ggccaccgac cgagtcgacg tcgtcctcgt cgaggtcgat gtcgaactcc tcggcgaagt
     8161 cgtcgacgtt catcgtcgcg gggatccgga agcgaccgtc cccgaggtct tcgactcccg
     8221 gtgtctcccg gtcgtattcg tcgtcgatct cgccgacgat ctcctcgatg atgtcctcga
     8281 tggtcaccag gccagcggtg ccgccgtatt cgtccacgac gatggcaatg tgcaccgagc
     8341 cgagttgcat ctcccggagc aggtcgtcga ccggtttcga ctcgggcacg aagggcatcg
     8401 gccgcatgat ggaggcgata ggaagcgtgc tgagttcgac tccgccggaa gtcatgtggc
     8461 ggaccaggtc cttgaggtag acaagcccga cgacatcgtc cggtccgtcg ccgacgaccg
     8521 gcagccgcga gaacccggag cggttgaaga gcgacatcgc ctggcgcccg tgcttgccca
     8581 cctcgatgga gaccatgtcg gtgcgcggca ccatgacctc gcgcgccacg gtgtcgccga
     8641 gttcgaagac cgaatgcagc atctctcgct cgtcgtcctc gatgagcgag ttctcccccg
     8701 cgatgtcgac cagatcgcgc agctccgatt ccgactggaa ggggccgtcg cgatagccgc
     8761 ggcccggggt gaccatgttg gcgatcagca ccaggaggcg cgcgaccgga ccaaggatgc
     8821 gatgtagcca tgcgatcagc ggcgcgaggg cgagcgccac ccggtcggag tgctggcgac
     8881 ccagcgtccg tggcgaaaca cccacggcga caaaggaaac caatgacatg atgccgatcg
     8941 agatcagcaa cgttcgccag aaactgtcgg tgagcgaact caccgacaag gtgatgcaca
     9001 cggcggcgac tgcttcggcg accgcgcgca agaatgcggt caccgagaga taggcggcgc
     9061 tgtcgccgag gatcttggcc agggagcggg ccccggcgcg gccctcgtcg ccgagctcct
     9121 cggcgcgcac ccggctggag cggctgatgg cggcctcacc gagcgcgagg acgaaggcga
     9181 agaggatcgc ggcgatcgcg acgaggatca ggcgggtggg catcgtcagc cggccggccg
     9241 gccgcgggag gcgaggaagg tcaacagcag ctggcgctgc aggtcgaaca tctccttctc
     9301 ctccgcgggc tcggcgtggt cgaagccgag caggtgcagg atgccgtggg tggtgaggag
     9361 cagcatttcc tccgctgggg cgtggccggc ttcggcggcc tgcttggtgg cgacgctcgg
     9421 gcacagcacg atgtcgccga gcacaccctc ttcggctgcc tcgccctccc ggccgggacg
     9481 cagctcgtcc atcgggaagc tcatcacgtc ggtcgggccg gcaaggtcca tccactgctc
     9541 gtgcaggacg gtcatcgtgg cctcgtcgac gagacggaca cacagctccg cctgggggtg
     9601 gacccgcatc gcggccatca cgtgccggga gagatcgacc agttccacct cgtcgacgac
     9661 gtagtcggtc tcattgagga cgtcgacgct caccggcggc ctttcgcgtc gtcggggcgt
     9721 gcgcggtctt ggtgtcccag cgcccgtagg cgtcgacgat ctcacccacg aggcggtggc
     9781 gcacgacgtc ctgtgaggtc agttgcgaga agtggacgtc ctcgatgccc tggaggatct
     9841 cctgcaccac gcgcaggccg ctctgggtgc cgccgggcag gtcgacctgg gtgacatcgc
     9901 ccgtcacgac catcttggag ccgaagccga ggcgggtcag gaacatcttc atctgctcgg
     9961 gcgaggtgtt ctgggcctcg tcgaggatga cgaaggcgtc gttgagagag cggccgcgca
    10021 tgaacgccag gggtgcgacc tcgatggtgc cggccgccat caggcggggg atcgactcgg
    10081 gatcgatcat gtcgtggagg gcgtcataga gcggccgcag gtaggggtcg atcttgtcgg
    10141 tcagcgtgcc gggcaggaac cccagccgct cgcccgcctc gacggccggc cgggtcagga
    10201 tgatccggtt gacctgcttt gcctgcagcg ccgcgacggc cttggccatc gcgagatagg
    10261 tcttgccggt gccggccggg ccgatcccga agacgatggt gtgggcatcg atcgcgtcga
    10321 catagcgctt ctggttgagc gtcttgggac ggatggtgcg accgcggttg gagacgatgt
    10381 tcatcgtcag cacatccgcc ggccgctccg tcgtctgcgc ccggagcatg ccgatcgagc
    10441 gctcgacggc gtcgcggttg agcggctgac cgccggcgat gacggcgagc aactcatcga
    10501 tcgtgcgctc gacgatagcg acgtcggcgc tcggaccgct catgcggaac tcgttgccgc
    10561 ggacgaggat ctcgacggcg gggaattgcc gctcgagcgt gcgcaggagt tcgtcgcggg
    10621 ggccgagcag ggagaccatc gcgacgctgg atgggatctc gacggtgtgc atggggtgca
    10681 ccggctgcgg caggccgctc gcctgggatg gatggtcact catgggtccg gccattctag
    10741 gcggggtctc acccaggggg ccacgccatt gtccggccac ccagcaggtg gaagtggcag
    10801 tggaagacgc tctggccggc gtcggcgccc gtgttggtca ccaggcggta tcccgcgccg
    10861 agcccctcgt cctgcgccac ctgggcggcg agcgcgacca ctgccgccag gtcccccggg
    10921 tgctcgccga gctccgccgc cgtggcaacg tgcttcttgg ggatcacgag cacatgcgtg
    10981 ggcgcctgcg ggttgatgtc gcggaaggcg agtgccagct cgctctccgc cacgacgtcg
    11041 ggctcgatgt cgcgggcgat gaacttgcag aacaggcagt cctcactcat acggtccaac
    11101 ctagcgccgc gccaggccgc gaaacctggg agcgggaccc ggcctggccg agacgggcgc
    11161 ggctttcgtt gtcgggccgg cctatttccc ggcccgaca
//
LOCUS       DAOPJG010000108        11086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6183_length_11086_cov_7.842807, whole genome shotgun sequence.
ACCESSION   DAOPJG010000108 DAOPJG010000000
VERSION     DAOPJG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6183_length_11086_cov_7.842807"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="PKU75_10500"
     CDS             <1..108
                     /locus_tag="PKU75_10500"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPF81352.1"
                     /translation="EYVGPLVDSQMHEKPLSIALREINDNLLTSEKLDA"
     gene            123..1448
                     /locus_tag="PKU75_10505"
     CDS             123..1448
                     /locus_tag="PKU75_10505"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0071513 - phosphopantothenoylcysteine
                     decarboxylase complex [Evidence IEA];
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate synthase"
                     /protein_id="HPF81353.1"
                     /translation="MNIVLGVGGGIAAYKAALLLRLFTESGHSVTVIPTAAALHFVGE
                     PTWSALSGKPVQTDVWTHVEQVPHVRLGQQADLVVIAPATADLLARAAAGLADDLLTN
                     TLLTARCPVVYAPAMHTEMWEHPATQANVETLIERGAHIVPPASGRLTGADTGPGRLP
                     DPEEIFAVCRHLLEHGASPSAGEDSGRGAPAIRAATGTRPGPPARRTSSPRQQEPDYT
                     GWHVLISAGGTREPLDPVRFLGNRSSGKQGFALAARAAARGALVKVVSANVSLPVPEG
                     VEVIPVGTALEMQAAVEAQVGWADLVIMAAAVADFRPAAYAGSKIKKTHDPADPDAAP
                     VIELVRNPDILAGLVQARGPAPRPVIVGFAAETGDADHSVLRLGEEKLARKGCDLLVV
                     NEVGVDKTFGSDENTVHILRKVGAPVAAGPAGKDVIADAILDAARPLMG"
     gene            1562..2782
                     /gene="metK"
                     /locus_tag="PKU75_10510"
     CDS             1562..2782
                     /gene="metK"
                     /locus_tag="PKU75_10510"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPF81354.1"
                     /translation="MSARLFTSESVTEGHPDKICDQISDSILDAMLEQDPRSRVAVET
                     MVTTGLVHVSGEVTTESYVEIPRLVRERILAIGYDSSAKGFDGASCGVSVSIGQQSPD
                     IAQGVDDAYEARTGTTDALDKQGAGDQGLMFGYACDDTAELMPLPIYLAHRLAERLTA
                     VRKANDLAYLRPDGKTQVTIEYAGDKAVRLDTVVVSTQHADGISLDALLAPDIESHVI
                     APVLAELAAGGTELDTSDYRTLINPTGKFVIGGPMGDAGLTGRKIIVDTYGGMARHGG
                     GAFSGKDPSKVDRSAAYATRWVAKNVVAAGLARRCEVQVAYAIGKAQPVGLYVETFGT
                     ETEPIEKIQSAITSVFDLRPAAIVHDLDLLRPIYVPTAAYGHFGRTMADGVDNPFTWE
                     KTDRVEELQRAVRG"
     gene            2841..4997
                     /locus_tag="PKU75_10515"
     CDS             2841..4997
                     /locus_tag="PKU75_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775101.1"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HPF81355.1"
                     /translation="MAKGVSDPGAGKTPTLDIATPAAPDRAVSEAAPQVAKPVASVLV
                     DSGLPHLDRFFEYAVPPELDEAARPGVRVKVRFAGQDLPGFVVERRARAEHPGRLAPV
                     RTVVSPEPVLTAPILALARELAAMYAGTPGDVLRLAIPPRHARAEKALPLEPPGLDPF
                     VGAQPWESFVGDYGGIDDRGVGFEMLTQLRKGRGGRWAACLAPAAGDPDLDWPAALAQ
                     TAAQVLQSGRGTIIVVPDHRDVDRLDAAVKVVLGPGRHVRLTADQGPQARYTSWLKVL
                     RGHVQVVIGTRAAAYAPVADLGLIAVWDDGDDLHEEPRAPYVHTRDVAATRARLEDAS
                     LLLTGYGRSVADQSLVEAGWLESMGPGRRWLREVAPRVVVAGAEREQERDPAAQSARL
                     PNLAWRTAKSALERGPVLIQVPRRGYVPSLACQECRTHARCLACGGPLGLDGGSGPPV
                     CRWCASVARAWRCPHCEGTRLRSLVVGARRTAEELGRALPGVPVLTSGAGTVLSHVPN
                     RPALVIATPGAEPVAEGGYAAALLLDAWALLDRVALDAAPEALRRWMAAAALVRAAVQ
                     EGTVVVCGAPLDQPAPAVQALVRWDAPWLARRELAERRQAQLPPAMIFAEAVGPRKVL
                     EQIAARADLPAGVARFGPVPKAASAAPAVPAGARAAATVRAAAGAGEAAYRLLLRAQP
                     ADADAMATALRAVRAERSARKEPDSLRIRVGLTDPD"
     gene            5160..5540
                     /locus_tag="PKU75_10520"
     CDS             5160..5540
                     /locus_tag="PKU75_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81356.1"
                     /translation="MSVVGSVRELGGFAIRSQLCARHTKRQIAAAVRRGEVRRPGRNR
                     YVVPEVDDQRAFAHEHSAILGVLSAALARVWKVKWVPDIVWPDVMYKPAWVRWVIETW
                     LATEDGMPLPDMPPGAPIIRDRAA"
     gene            5729..6274
                     /gene="def"
                     /locus_tag="PKU75_10525"
     CDS             5729..6274
                     /gene="def"
                     /locus_tag="PKU75_10525"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPF81357.1"
                     /translation="MPVQPIRLFGDPVLRTRADEVVDFDKELRTLVQDLTDTMLDAPG
                     AGLAAPQIGVGLRVFVYHVDDIVGHLINPELALGEEEQDGPEGCLSFPELRYDTKRSL
                     NVVARGMNMWGEPVFLEGTELLARAIQHETDHLDGILFIDRLDRETRKLAMKAIREAD
                     WGPGGPPVVKVSPHSTFGQAL"
     gene            6292..7257
                     /gene="fmt"
                     /locus_tag="PKU75_10530"
     CDS             6292..7257
                     /gene="fmt"
                     /locus_tag="PKU75_10530"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPF81358.1"
                     /translation="MRLIFAGTPDVALPALDALAATEHVIAAVITRPDAPAGRGRKLI
                     PSPVKARALELRLEVWEPARLSDEDFLSRLRAAEPDLCPVVAYGNLIPQAALDVPRHG
                     WVNLHFSLLPAWRGAAPVQHAVIAGDEITGASTFLIEAGLDTGPVYGSLTEPVRPTDT
                     SGDLLGRLADAGAGLLVATVDGIARGELVPVPQPTDGVSLAPKLDVADARIDFTHPAL
                     AVDRRIRGCTPAPGAWTTFRGERLKLYPVTVASGSDSPLAPGELRASKSAVEVGTATV
                     PVTLGQVQPLGKKPMPAADWARGVRIEPGERLGNSASTRPAESAG"
     gene            complement(7306..8022)
                     /locus_tag="PKU75_10535"
     CDS             complement(7306..8022)
                     /locus_tag="PKU75_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF81359.1"
                     /translation="MPDVRLILLHGTQLNSAQWLGYADLLGPRIELVTPDLPAHGIRR
                     DEPFTWESTMALVDAAVAGTDLPVVLGGHSLGGYLAAAYAADHLTRLAGLALLGAAGE
                     PGGMGSASYRTIARINRRLGPARMTRILDFDLRKLGTPPAMADALLARGYAFDGVTPA
                     WSLIMEKCSSSQLAEVTCPILLCAGQFDQLRMHTRRYAAAARSSSDVRVVTIPRASHA
                     FPLTHAGQTAAELRRLCRVE"
     gene            complement(8041..8733)
                     /locus_tag="PKU75_10540"
     CDS             complement(8041..8733)
                     /locus_tag="PKU75_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPF81360.1"
                     /translation="MTTTSDEIELRDPYANAYNPAAERERARQSTHGAPRKQRERRRG
                     WATAAAIGIPLLVMGGIGAAVINGYNDALAAREEAASPSTTAADAQAGPSDHAPTGPD
                     VGTTGLDAQLTRAITRAREAAAAEGVTIDITSGARTAEQQQVLFDNAVAKYGSAAEAS
                     KWVLPPDKSNHVLGKAVDVGPYAAAVWLQKNGVKFGLCQAYANEYWHFEVLAPAKGQK
                     CPAPVANAAAGG"
     gene            9023..10417
                     /locus_tag="PKU75_10545"
     CDS             9023..10417
                     /locus_tag="PKU75_10545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HPF81361.1"
                     /translation="MAAYTVMRSIASGGYANLDLPKELRQRRLSGRDAAFATELTYGA
                     TRLRGIYDRIIEIAADRPIARIDGGVLDTLRLGAHQLLGMRVPPHAAADTSVALARQV
                     NGAGAAGLVNAVLRRISEADLEEWLTRIVPADADATTKAAIQHSHPEWIAKALRQALL
                     GHGASTPATVDADLAAVLAADNAAAEVNLVARPGLVTVEELAVDGATAHPHVPTAALL
                     PSGEPGQLAAVRDGRAAVQDAGSQVVALTLAAAPLVRAAHGRERWLDLCAGPGGKAGL
                     LAALAIEQRADLVANEVQPHRAELVAATLRVASERAEGAGLTLEVRTGDGRDYGVDEP
                     AAYDRILIDAPCTGLGALRRRPEARWRRQPSDLAALGSLQRELLASALDALAPGGVAV
                     YATCSPHLAETTFVVGDVLKKRSDIEHLDARPVVEEVTCGWGGTLGAGPDVQLWPHVH
                     GTDGMFLALLRKPG"
     gene            10443..>11086
                     /gene="rpe"
                     /locus_tag="PKU75_10550"
     CDS             10443..>11086
                     /gene="rpe"
                     /locus_tag="PKU75_10550"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPF81362.1"
                     /translation="MQISPSILSADFANLESELDAIATADWAHVDVMDGHFVPNLTIG
                     LPVVKRLVEVSPVPIDTHLMIEDPDRWAPMYAELGAKSVTFHIEAAREPRTLAKNLRA
                     LGARASMALKPGTPWAPYADLLPELDMVLVMTVEPGFGGQAFMADQMPKVREVREAVR
                     RFGGEVWIQVDGGVSASTIEQCVEAGADVFVAGSAVYNADDAAANIAHLRELVAA"
BASE COUNT         1536 a         3954 c         3991 g         1605 t
ORIGIN      
        1 gagtacgtcg gcccgctcgt cgactcgcaa atgcacgaga agcccctgtc gatcgcgctg
       61 cgcgagatca acgacaacct gctgacctcc gagaagctcg acgcctgacg gctgcccgcg
      121 tcgtgaacat cgtcctgggc gtcggtggcg gcatcgccgc ctacaaggcg gccttgctgc
      181 tgcgcctgtt caccgaaagc ggtcacagcg tcaccgtcat cccgaccgct gccgcgctgc
      241 atttcgtcgg tgagccgacc tggtcggccc tatccggcaa gcccgtgcag accgacgtct
      301 ggacgcacgt cgagcaggtg ccgcacgtgc gcctgggcca acaggccgac ctcgtcgtca
      361 tcgcccccgc caccgccgac ctgctcgcgc gggccgcggc cggcctcgcc gacgacctgc
      421 tcaccaacac cctgctgacc gcgcgctgcc cggtcgtcta cgccccggcg atgcacacgg
      481 agatgtggga gcacccggcg acccaggcga acgtcgagac gctcatcgag cggggggccc
      541 acatcgtgcc gcccgcgtcg ggccggctca ccggcgccga caccggtccc ggccgcctgc
      601 cggatccgga ggagatcttc gcggtatgcc gccacctcct cgagcatggt gcgtcgcctt
      661 ctgctgggga ggattcgggc cgcggtgcgc cggctatccg tgccgcgacc ggtacccgtc
      721 cgggtcctcc cgcccgccgc acgtcctcgc cgcgtcagca ggaaccggac tacaccggct
      781 ggcatgtcct gatcagcgcc gggggcaccc gggagccgct cgatccggtg cgcttcctcg
      841 gcaaccgcag ctcgggcaag caggggtttg ccctcgcggc ccgggccgcc gcccgggggg
      901 ccctggtgaa ggtcgtgagc gccaatgtgt ccttgccggt gcccgagggc gtcgaggtca
      961 tcccggtcgg caccgccctg gagatgcagg cggcagtcga ggcgcaggtg ggctgggccg
     1021 acctggtgat catggcggca gcggtggcgg acttccggcc cgcggcatac gccggcagca
     1081 agatcaagaa gacccacgac ccggctgacc ccgatgccgc ccccgtcatc gaactggtcc
     1141 gcaatcccga cattctcgcc gggttggtgc aggctcgcgg gccggccccg agaccggtca
     1201 tcgtcggatt cgccgccgag accggggacg ccgaccactc ggtgctgcga ctgggggagg
     1261 agaagcttgc ccgcaagggc tgcgacctgc tcgtcgtcaa cgaggtcggt gtcgacaaga
     1321 ccttcggttc ggacgagaac accgtccata tccttcgcaa ggtcggcgca cccgttgcgg
     1381 ccggcccggc cggcaaggac gtcatcgccg acgccatcct cgacgccgcg cggcccctga
     1441 tggggtgacc ggtcgacata ccgtcgtcca ccacgcgagc ctgccgcaag tgcgccccag
     1501 ccgctgttta gaatcgataa gttgcgcctg ccgcgcgtct tcgaacacca ggagttaatt
     1561 cgtgtctgca cgcctgttca cctccgagtc ggtcaccgag ggacaccccg acaagatctg
     1621 tgaccagatc tctgactcga tcctcgacgc catgctcgag caggacccgc gcagccgggt
     1681 cgccgtcgag acgatggtca ccacgggtct ggtgcacgtc tcgggtgagg tgaccaccga
     1741 gtcgtatgtc gagatcccgc gcctggtgcg tgagcggatc ctggcgatcg gctatgactc
     1801 gtccgccaag ggcttcgacg gcgcctcgtg cggtgtgtcg gtgtccatcg gccagcagag
     1861 ccccgacatc gcgcagggtg tcgacgacgc ctacgaggcg cgcacgggca cgactgacgc
     1921 cctcgacaag cagggcgccg gcgaccaggg cctgatgttc ggctacgcgt gcgacgacac
     1981 ggcggagctg atgccgctgc cgatctatct cgcccaccgt ctcgccgagc gcctgaccgc
     2041 ggtgcgcaag gccaacgacc ttgcatacct gcgcccggac ggcaagaccc aggtgacgat
     2101 cgagtacgcc ggcgacaagg ccgtccgcct cgacaccgtc gtcgtctcca cccagcacgc
     2161 cgacggcatc tccctcgacg cgctcctcgc gccggacatc gaaagccacg tcatcgcgcc
     2221 ggttctcgcc gaacttgcgg ccggcggcac ggagctcgac acctccgact accgcaccct
     2281 gatcaacccc accggcaagt tcgtcatcgg cggaccgatg ggggatgcgg ggttgaccgg
     2341 gcggaagatc atcgtcgaca cctacggcgg gatggcccgg cacggtggtg gggcgttctc
     2401 cggcaaggac ccgtccaagg tggaccgctc cgcggcctac gcgacccgct gggtggccaa
     2461 gaacgtcgtc gccgcgggcc tggcgcgccg gtgcgaggtc caggtcgcct atgcgatcgg
     2521 caaggcccag ccggtcgggc tctatgtgga gaccttcggc accgagaccg agccgatcga
     2581 gaagatccag tccgcgatca cctccgtttt cgacctgcgt cccgccgcga tcgtgcacga
     2641 cctggacctg ctccgcccga tctacgtccc caccgccgca tacggccact tcggccgcac
     2701 catggccgac ggcgtcgaca accccttcac ctgggagaag accgaccgcg tcgaggagct
     2761 ccagcgcgcc gtccgcggct gagacggggt tcgtctcccg cacgtgggct tgtcgggacc
     2821 ggcggctatc gtcggtcgct atggcgaagg gcgtgagcga tccgggcgcg gggaagaccc
     2881 cgacgctcga catcgcgacc cctgcggccc cggaccgggc ggtctccgag gctgcgccgc
     2941 aggtcgccaa gccggtcgcg agcgtgctcg tcgacagcgg actgccgcac ctggaccggt
     3001 tcttcgagta tgccgtgccg cccgaactgg acgaggcggc ccggcccggg gtgcgggtca
     3061 aggtgcgctt cgccgggcag gacctgcccg ggttcgtcgt ggagcgccgc gcgcgtgccg
     3121 agcatcccgg ccggctggca cccgtccgca ccgtggtctc gcccgagccc gtgctcaccg
     3181 cgcccatcct cgcgctggcc cgggagctgg cggcgatgta tgccggcacc cccggcgacg
     3241 tgctgcgtct ggccatcccg ccgcggcacg cccgcgccga gaaggcgctg ccgctcgagc
     3301 cgcccggcct cgaccccttc gtgggtgcgc agccgtggga gtccttcgtc ggcgattacg
     3361 gcggcatcga tgaccgcggg gtgggtttcg agatgctcac ccagctgcgc aagggccgcg
     3421 gcggacgctg ggccgcctgc ctcgccccgg ccgcgggcga tcccgatctc gactggccgg
     3481 ccgcgctcgc ccagaccgcc gcccaagtgc tccagtccgg tcgcggcacc atcatcgtgg
     3541 tgcccgatca ccgcgacgtc gaccggctcg atgccgccgt gaaggtggtg ctcgggccgg
     3601 gccggcacgt ccggctgacg gcggatcaag gtccgcaggc gcgctatacg agttggctga
     3661 aggtgctgcg ggggcacgtg caggtcgtca tcggcacccg cgccgccgcc tatgcacccg
     3721 tcgcggatct cgggctgatc gcggtctggg acgacggcga cgacctgcac gaggaaccgc
     3781 gtgcgcccta tgtgcacacc cgcgacgtgg cggcgacacg cgcgcgcctc gaggacgcgt
     3841 cgctgctgct gaccgggtat ggccgttcgg tcgccgacca gagtctcgtc gaggcgggct
     3901 ggctggagtc gatggggccg ggacgcaggt ggctgcgcga ggtcgcgccc cgcgtcgtgg
     3961 tcgccggtgc ggagcgtgaa caggagcggg acccggcggc gcagtcggcc cgcctcccga
     4021 atttggcttg gcgcacagcg aaatccgctc ttgagcgcgg gccggtgctg attcaggtgc
     4081 cgcgccgcgg ctatgtgccc tcgcttgcgt gtcaggagtg ccgcacccac gcccgctgcc
     4141 tcgcgtgcgg cggtccgctc gggctggatg gcgggtccgg gccgccggta tgccgctggt
     4201 gcgcctccgt cgcccgcgcc tggcgctgcc cgcactgcga gggcacccgg ctgcgttcgc
     4261 tggtggtggg tgcgcgccgc accgccgagg agctggggcg ggcgctgccg ggggtgccgg
     4321 tgctgacgtc cggggcgggc accgtgctct cgcacgtccc gaatcgccct gccctcgtca
     4381 tcgcgacgcc cggcgccgag ccggtcgccg agggcgggta tgccgccgcc ctcctcctcg
     4441 acgcctgggc cctgctcgac cgggtcgccc tcgacgccgc acccgaggcg ctgcgccggt
     4501 ggatggccgc ggcagcgctc gtgcgggcag cggtgcagga gggcaccgtg gtcgtctgcg
     4561 gcgctccgct cgaccagccc gcaccggcgg tccaggccct ggtgcggtgg gacgcgccgt
     4621 ggctcgcccg gcgtgaactg gccgagcgcc ggcaggccca actgccgccg gcgatgatct
     4681 tcgccgaggc cgtcgggcct cgaaaagtgt tggagcagat cgctgctcgc gccgacctgc
     4741 ccgcgggcgt ggcccgcttc gggccggtgc cgaaggccgc ctccgcggcc ccagcggtgc
     4801 cggcgggggc gcgggcggcg gccacggtgc gggcggcggc gggtgcgggt gaggcggcat
     4861 accgtctgct gttgcgggcc cagcccgcgg acgccgatgc gatggccacg gccctgcgcg
     4921 ccgtgcgggc cgaacgcagc gcccgcaagg agcccgactc gctgcggatc cgagtcggcc
     4981 tgaccgaccc cgactgaccg caggcagggc gtgcgcagcg gtccgccaac taccggaagt
     5041 ccctgtggga ctggatcgcc aacgggggat cgcggcttga ctgcgagttc gtgtcgtcgg
     5101 cggcccgggt tgtccacagg ggagcgccgg atcggccgaa tgacgacaga gtgcatggca
     5161 tgtccgtggt cggcagtgtg cgtgagttgg gcggattcgc catccgcagc caattgtgcg
     5221 cgcgccacac caaacgccag atcgcggcgg cagttcggcg gggcgaggtg cgccggcccg
     5281 gccgcaaccg ctatgtggtc cccgaagtgg acgaccagcg cgccttcgcc cacgaacact
     5341 cggcgatcct tggcgtcctg agtgcggcgc tggctcgcgt ttggaaggtc aagtgggttc
     5401 cagacatcgt gtggccggac gtgatgtaca agccggcctg ggtgcggtgg gtgatcgaga
     5461 cctggctcgc gacggaagac ggtatgccgt tgccggacat gcccccaggc gctccgatca
     5521 tccgcgaccg cgccgcgtga ccgcggtctc tgcctggctg atcctggcgc cggtatccgc
     5581 gagggcgggc cactgacgac acgacgtccg cacgtggcgg cgctcgagcg ggggcggcag
     5641 cacatcgtgg acttcatgta gtcgggggtc cgcggttggg ccgcggtggg gccacgaggc
     5701 atgggcggca cccgcataca cttccggggt gcccgtccag cccatccgcc tcttcggcga
     5761 ccccgtgctg cgcacccgcg cggacgaggt cgtcgacttc gacaaggaac tgcgcaccct
     5821 cgtccaggac ctgaccgaca ccatgctcga cgctcccggt gccgggctcg ccgccccgca
     5881 gatcggggtg gggctgcggg tcttcgtcta tcacgtcgac gatatcgtcg gtcacctcat
     5941 caaccccgag ctcgcgctgg gcgaggagga gcaggatgga cccgaaggct gcctgtcctt
     6001 ccccgaattg cgttatgaca caaagagatc actcaatgtg gtggcgcgcg gcatgaacat
     6061 gtggggcgag ccggtcttcc tcgagggcac cgagttgctc gcccgcgcca tccagcacga
     6121 gaccgaccat ctcgacggca tcctcttcat cgaccggctc gaccgggaga cccgcaagct
     6181 cgcgatgaag gcgatccggg aggcggactg ggggccgggt gggccaccgg tcgtcaaggt
     6241 ctcaccgcac tcgacgttcg gccaagcgtt gtgaaccggg gtgcgatcta gatgcgactg
     6301 atcttcgcgg gcacgcccga cgtggccctg ccggccctcg atgccctcgc ggcaaccgag
     6361 cacgtgatcg cggcagtgat cacccgcccc gacgcccccg ccgggcgggg ccgcaaactg
     6421 atcccctcgc cggtgaaggc tcgggctctg gagttgaggc tggaggtgtg ggagccggcc
     6481 cggcttagcg acgaggactt cctcagtcgc ctgcgggccg ccgagccgga cctgtgcccc
     6541 gtggtcgcct acggcaacct catcccgcag gcggcgctcg acgtgccccg tcacggctgg
     6601 gtgaatctcc acttctcact cctgccggca tggcggggcg cggcgccggt gcagcacgcc
     6661 gtgatcgcgg gcgacgagat cacgggcgcc tcgaccttcc tgatcgaggc agggttggac
     6721 accgggccgg tctacgggtc gctgaccgag ccggtgcgcc cgaccgacac ctccggcgac
     6781 ctgctcggcc ggctcgcgga tgcgggcgcc ggcctgttgg tcgcgaccgt cgacgggatc
     6841 gcccgcggtg aactcgtgcc cgtcccgcag cccaccgacg gcgtcagcct ggcgcccaaa
     6901 ctcgatgtcg cggacgcccg catcgacttc acccacccgg cgctggcggt ggaccggcgg
     6961 atccggggct gcacccccgc cccaggggcc tggacgacct tccgtggcga gcgcctcaag
     7021 ctttaccccg tgacggtcgc gtctgggtcg gactccccac tggccccggg ggaattgcgc
     7081 gcgagcaagt ccgccgtcga ggtgggcacg gccaccgtcc ccgtcacgct aggccaggtc
     7141 cagccgctgg gcaagaaacc gatgccggcc gccgactggg cccgaggcgt gcgcatcgag
     7201 cctggcgaac gactggggaa ctcggcttcc acccgcccgg ccgagagcgc cggctaagtc
     7261 cgacacccgg cgcgcgcggg gtcgtcagat gtcggactca acgactcact cgacgcggca
     7321 gaggcggcgc aactccgcgg cggtctggcc ggcgtgggtg agcgggaagg catggctggc
     7381 gcgggggatc gtgaccaccc ggacgtcgga ggaggagcgg gcggccgcgg cataccggcg
     7441 cgtgtgcatc cgcaactggt cgaattgccc ggcgcacaac aggatcgggc aggtcacctc
     7501 ggcgagctgg ctcgagctgc acttctccat gatcagcgac caggcgggcg tcacgccgtc
     7561 gaaggcgtag ccgcgggcca ggagggcgtc tgccatggct ggcggggtgc cgagtttgcg
     7621 caggtcgaag tcgaggatgc gggtcattcg cgccggcccg agccggcggt tgatccgcgc
     7681 gatcgtgcgg tagctcgccg accccatacc ccccggttcg ccggcggcgc ccagcagggc
     7741 aagcccggcg aggcgggtga ggtggtccgc ggcatatgct gcggcgaggt agccgccgag
     7801 gctgtgcccg cccaacacca ccgggagatc ggtcccagcg accgccgcgt cgacgagcgc
     7861 catcgtcgac tcccacgtga acggctcgtc gcgtcgtatg ccgtgggccg gcagatcggg
     7921 cgtcaccagt tcgatccggg ggccgagcag gtcggcatac ccgagccact gggcggagtt
     7981 gagttgggtg ccgtgcagca ggatcaggcg gacgtcgggc acgttgtcat tgtgcctgcg
     8041 tcaaccgccc gcggccgcgt tggcgacggg cgccgggcac ttctgcccct tcgccggggc
     8101 gaggacctcg aaatgccagt actcgttggc gtaggcctgg cacaggccga acttgacgcc
     8161 attcttctgc agccacaccg cggcggcata ggggcctaca tcgaccgcct tgccgagcac
     8221 gtggttcgac ttgtccggtg gcagaaccca tttcgaggcc tcggctgcag acccatattt
     8281 cgccacggca ttgtcgaaga gcacctgctg ctgctcggcg gtgcgggcgc cggaggtgat
     8341 gtcgatggtg accccctccg cggcagcggc ctcgcgggcg cgggtgatgg cccgggtgag
     8401 ctgggcgtcc aggcccgtcg tcccgacgtc gggaccggtg ggggcgtggt cgctggggcc
     8461 ggcctgagca tccgcggccg tggtcgacgg gctggcggcc tcctcgcgcg cggcgagggc
     8521 gtcgttgtaa ccgttgatca ccgccgcgcc gatgccgccc atgacgagca gcggtatgcc
     8581 gatcgccgcc gcagtcgccc atccccgccg gcgctcgcgt tgcttccgcg gcgcgccgtg
     8641 ggtggactgg cgggcgcgtt cgcgctcggc cgccgggttg taggcgttcg cgtagggatc
     8701 gcgcagttcg atctcgtcgc tcgtcgtcgt cacagggacc ctccacatcg gttcatgccg
     8761 aagacaacgt ataccgggta tgcgaggttt ccgtgggcgc cacgccgcac acgcgcgtcg
     8821 acccttaggc ttctggcccg tgagtgagca gcgtgggcgc ggcgacggga gccgcgcaga
     8881 tgggcgccgt ggcgatcgcg accgatccgg aggtggcgga cgcggccgag gtggccggcc
     8941 gccggaagga agcgcgcgcc ctcacgggca aaggtccgcc cagcgcccga gccaacgtgg
     9001 ccgcaccggc gaccccgcgc ggctggcggc ctacacggtc atgcgctcca tcgcctccgg
     9061 cggctacgcc aatctcgact tgcccaagga attgcgccag cggcgcctga gcgggcggga
     9121 tgccgcgttc gcgaccgaac tgacatacgg cgcaacacga ttgcgcggca tctatgaccg
     9181 catcatcgag atcgcagcgg accggccgat cgcgcgcatc gacgggggag tcctcgacac
     9241 gctgcggctc ggtgcccatc aactgctggg tatgcgggtg ccaccgcacg cggccgccga
     9301 cacgtccgtc gcgctggcgc gccaggtcaa cggggcggga gcggccggcc tcgtcaacgc
     9361 ggtcttgcgc cggatcagcg aggccgatct cgaggagtgg ctgacccgga tcgtgccggc
     9421 cgacgccgac gcgaccacga aagcggccat ccagcactcg caccccgagt ggatcgccaa
     9481 ggcgttgcgg caggcgctcc tggggcatgg cgcgtcgacg ccggcgactg tcgatgccga
     9541 cctggcggcg gtgctggcgg ccgacaacgc tgcggcggag gtcaacctgg tcgcgcgccc
     9601 cgggttggtg acggtcgagg agttggccgt cgacggagca acggcgcacc cgcacgtgcc
     9661 gacagccgcg ttgttgccca gcggtgagcc gggccaactg gcagccgtcc gtgacggccg
     9721 cgcggcggtg caggacgcgg gttcgcaggt cgtcgccctc acgctcgcgg ccgcacccct
     9781 cgtcagagcg gctcacgggc gcgaacgctg gctggacctg tgcgccggcc ccggcggcaa
     9841 ggcgggtttg ctcgccgcgc tcgcgatcga gcagcgagcg gacctggtcg ccaacgaggt
     9901 tcagccgcac cgagcggaac tggtcgccgc gacgctgcga gtggcgagcg agcgtgcgga
     9961 gggagccggc ctgacgctcg aggtccgcac cggcgacggg cgagactacg gagtggacga
    10021 gcccgcggca tacgaccgga tcctcatcga cgcgccgtgc accggacttg gcgcgctccg
    10081 gcgccgcccg gaggcgcgct ggcgccgcca gccctccgat ctcgccgcac tgggatcttt
    10141 gcagcgcgag ctgctcgcca gcgccctcga cgccctcgcc cccgggggcg tggcggtgta
    10201 tgcgacgtgc agcccgcacc tcgccgagac caccttcgtc gtcggggacg tgctcaagaa
    10261 gcggtccgat atcgagcacc tcgacgcccg gccggtcgtc gaggaggtca cctgcggatg
    10321 gggcgggacg ctcggggccg gccccgatgt ccagctgtgg ccccatgtcc acggcacgga
    10381 cgggatgttc ctggcactgc tgcgcaagcc gggctgacgc cggccgtcga taggctggac
    10441 gggtgcagat ctcaccgagc atcctgtccg ccgacttcgc caatctcgag agcgaactcg
    10501 acgccatcgc cacggccgat tgggcacacg tcgacgtcat ggacgggcat ttcgtgccca
    10561 atctgaccat cggcctgccg gtcgtcaagc ggctcgtcga ggtcagcccg gtgccgatcg
    10621 acacccacct gatgatcgag gacccggatc gttgggcgcc gatgtatgcc gagctgggcg
    10681 ccaagtcggt caccttccac atcgaggcgg cgcgcgaacc ccgcactctc gcaaagaatt
    10741 tgcgtgcgct cggcgcacgg gcgtcgatgg cgctgaagcc gggcacgccg tgggcgccgt
    10801 atgccgacct gttgcccgaa ctcgacatgg tgctcgtcat gacggtcgag ccgggcttcg
    10861 ggggtcaggc gttcatggcc gaccagatgc cgaaggtgcg cgaggttcgg gaggccgtgc
    10921 gccggttcgg cggagaggtg tggatccagg tcgacggcgg cgtatccgcc tcgacgatcg
    10981 agcagtgcgt cgaggccggc gccgacgtct tcgtcgccgg gtcggccgtc tacaacgccg
    11041 acgacgcagc cgccaatatc gcccacctgc gcgaacttgt tgcggc
//
LOCUS       DAOPJG010000109        11086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6184_length_11086_cov_7.264799, whole genome shotgun sequence.
ACCESSION   DAOPJG010000109 DAOPJG010000000
VERSION     DAOPJG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6184_length_11086_cov_7.264799"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..552)
                     /locus_tag="PKU75_10555"
     CDS             complement(70..552)
                     /locus_tag="PKU75_10555"
                     /inference="COORDINATES: protein motif:HMM:NF025391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HPF81363.1"
                     /translation="MVPSPLTVVDFGYEATKLEPLERRVTPGSYAVDVAIAYDRCAAM
                     RIILGPGEPVSWHPAVSPDGGHVAGVDAGNIAVLDLPTYAGASVRDKERAYDDFTLNP
                     AQDSGLLLAFDGRPPIGAVSSSGWGDGAYPAYWGADAQGRPVQLVVDYLWLSARSTAP
                     "
     gene            692..1927
                     /locus_tag="PKU75_10560"
     CDS             692..1927
                     /locus_tag="PKU75_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81364.1"
                     /translation="MSRIGETVAPARMGRDFRWLLASSWVGQIGDGIALAAGPLLVAS
                     QTRNAVLVGLAAALQRVPTLLFGLVAGAVADRHDRKRLVVLSNAARMAVLGVLVATLA
                     TGHVNIAVVLAAMFALGCAEIFADTAFGTIMPMLVAKEDLGLGQSRLMAGFLTGNQLV
                     GPPVGAFLFAAGMAWPFVVQIVTLLLGLLLIVRIATTTHARPDREPTHVRRDIADGVR
                     WLLGNAPVRTLALVILVFNITWGAAWSVLVLYSLDHLHMGEVGFGLLTTAAAAGGIAG
                     TTSYGWLERHFALAALMRACLLLEVATHLALAMTTSGTVAMGIMVVFGTYAFVWGTLS
                     NAVRQRATPTEFQGRVSSVYLVGVFGGLVVGQLLGGVIAQRWGLTAPFWFAGVGSGLT
                     LLVVWRELAHIAHADSASD"
     gene            complement(1924..3051)
                     /locus_tag="PKU75_10565"
     CDS             complement(1924..3051)
                     /locus_tag="PKU75_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPF81365.1"
                     /translation="MPRSPRAGDMALEPHRLTAVQARRIVVRAQLLARSRPTSLLEVV
                     RHLAAVQVDNCRHVAPSHELVLWSRLGAQFDVAEVDRRLADGSLIEIGGMLRPGEDIA
                     LYRAEMAAWPGGERPWQQDLAEWVAVNDEGRRAIRHELEVEGPLPARALPDVTLVPWR
                     SSGWTNDKNTMRLLEMMARRGEVAVAGRGPDGREWDLAERVYPDSPTVPLEEALRIRD
                     ERRLTALGVARATVPEVFEDVSSAGAAGEPAVIEGVKGTWRVDPGQLGQSFGGRTALL
                     SPLDRLVFDRKRTLELFGFDYQLEMYKPAAKRRWGYWAMPVLHGDRLIGKVDAQTDRT
                     RGVLRVNAIHEDAPWPGAARGGIRRELAGLAEWLGVELQLP"
     gene            complement(3026..3244)
                     /locus_tag="PKU75_10570"
     CDS             complement(3026..3244)
                     /locus_tag="PKU75_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF81366.1"
                     /translation="MHYTQYDTRVAAYAVLVDADDRILLTWWNGEGRAEGLWSMPGGG
                     VEFDESVEEAVARDDIVDIAYAALTSGG"
     gene            complement(3251..4204)
                     /locus_tag="PKU75_10575"
     CDS             complement(3251..4204)
                     /locus_tag="PKU75_10575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPF81367.1"
                     /translation="MTRLGYQIPNFTYPGVDSGGLFDVIVAQAKEAEASGFDTVLVMD
                     HFYQLPMLGSPANEMLECYTLLSALAQHTETVRLSALVTGNTYRNPALLAKIVTALDV
                     VSHGRAQLGIGAGWFELEHDALGYEFGTFTDRFQKLEEALQIILPMLRGERPTLDGRW
                     YQVTDAINSPGPVGRIPVMIGGGGEKKTLRMVAQYADESNIICPVEDIPRKLEALAGH
                     CDRLGRDRAEITMTYQTSCVVAPTREEAEADFEAAVARTPELGMRRGSAIVGGPEEVA
                     AAYAKILDTGVDGVTVNMPANGHVPGRVALLGQTLAPLIAQ"
     gene            4394..5680
                     /gene="clpX"
                     /locus_tag="PKU75_10580"
     CDS             4394..5680
                     /gene="clpX"
                     /locus_tag="PKU75_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPF81368.1"
                     /translation="MARVGESSDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELAESTDLGFDELPKPREIFDFLQTYIIGQEPAKKALAVAVYNHYKRIQAGEAA
                     NKRDANHVEIAKSNILLIGPTGCGKTYLAQTLAKMLNVPFAIADATALTEAGYVGEDV
                     ENILLKLIQAADYDVKKAETGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILE
                     GTTASVPPQGGRKHPHQEFIQIDTTNVLFIVGGAFAGLEKLIESRNGKKGLGFGSELH
                     TPKDLAESIVDVMPEDLMKFGLIPEFIGRLPVITTVSPLDGDALVRILSEPKNALVKQ
                     YAKMFELDGVELEFTSEALTAIAEQALKRGTGARGLRAIMEEVLMPVMFDVPSDDDIA
                     RVVVTEETVLGNVLPTIVRQEHEPPTRKRSPRKRSA"
     gene            5734..6405
                     /locus_tag="PKU75_10585"
     CDS             5734..6405
                     /locus_tag="PKU75_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81369.1"
                     /translation="MTAPFRLHAGDSAPSQQSPTTCGAACLTVARMLIDPLFARWILT
                     GDGPRRGVPDAPTLGERFAAYERIVHRRTNGLYAGAGRLNLPWPRRLGTPPWGAKKEL
                     EFGAARRDTDYTVEMLRHHSPAELGEHFDRLLAIVADGEPALLYVGTHLVPRHVLLIL
                     PDNGDGNLEVYDPARGTVTELTRHAFTSRAVDLAGWDVPWFSVEPVGIVRERAYEWAP
                     GAVPA"
     gene            complement(6415..8286)
                     /locus_tag="PKU75_10590"
     CDS             complement(6415..8286)
                     /locus_tag="PKU75_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPF81370.1"
                     /translation="MTVLPYGTWPSPITPASLTAAQARLDDVAVDGADTYWLEGRPWE
                     EGRTVLMRHAGAAGERVEVTLAPWNVRSRVHEYGGGAYAVQSGIVVFSHFGDGRLRRL
                     DPGALEPVAITPEGEVRFGGLVLHGDWLYAVREDHRAGGEPVNELVRLDPHGDNADFG
                     TVLATGSDFVSRPAVSPDGTRVAWVSWDHPNMPWDSTTLHEASLTGAGASDATVIAGG
                     PGVSVSQPAYDPAGRLWFFSDETNWWTPWIVDAGRTRQVLGVEADLASPQWMLGMRDI
                     AFIDDGRAVIRDGAHGLTLVDDTGALTPLGVPSTHTETICATGDRVALKRGLADRLPE
                     VVSVETGTGDVTVLATSSPDVPDPGYVSRAEEVSWTNSAGLTAYGNFYPPVNAGHTGP
                     DGDLPPLLVFVHGGPTSAALPSYQTGLQYWTTRGFAILDVNHGGSTGHGRDYRERLRG
                     QWGVVDIDDCVTGAAAMAAQGRADSARLAIRGGSAGGYSTLRALTSGDVFAAGTSYFG
                     ISDLKVLLNDDHKFESRYTLGLVGPWPEAADVYADRSPINHVDEIHGELLLLHGADDM
                     VVPLAQTQQMADAMRAAGRDVELVIYPGEGHGFRAATTIEDAMTRELAHYQRVFATG"
     gene            complement(8302..9747)
                     /locus_tag="PKU75_10595"
     CDS             complement(8302..9747)
                     /locus_tag="PKU75_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HPF81371.1"
                     /translation="MSTTSFIGSAAAIAVGLSLATTTGAAGPDDTPDDALRAASGCTG
                     TAYVYGVRRDATLTFAAIDAATNVRTRKLTGPKLPWGVRAMTALNMNTLLVTTSQGAL
                     YRIDVTGNTKSLTIGAPVPMAYTGWNYDKLAADGAGHLFATGDTNGQLWRWDVSAKKP
                     TGSQINQKVRIGAGVIAKTLTGVGGGRLLATTSGGKLLGIRAAGAGNLTISTLGSSGW
                     GGVSALASPGGGLFFARTDSSGVLSTRRDADITNSSGTDISLIGYVGDGGWTQKLLTT
                     GPRNVVCPSGGITYDGLVTMFGSGWVGPRDIVERGLPGLQAEMTKGAINTPARKAAFL
                     ATLVSESAMRYDADESGNTQTYRGRGYIQLTNDFNYASAGTYFGINLLGQPDLAKSLH
                     YSAPIARWYWTVARTTTNAYADDHDMNGVNRNIGFAWSYEEATRRCDRFKSAYKYFTG
                     AYPTNTTCYPARLPARGDTDWYFHPERGDSR"
     gene            complement(9831..10367)
                     /locus_tag="PKU75_10600"
     CDS             complement(9831..10367)
                     /locus_tag="PKU75_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81372.1"
                     /translation="MTTVREWFAGQGRDRILLRLLILLAGLATTGLTSAAAPGGDLAF
                     VPYLVLAAIAAAAAPETWVTAVLALLHCGVWWLFVPRPESPAGLMLPAGAALLLLVMH
                     LAASACGVWPPGVRVPAEARMRWARDAGLVAVGILVTAALGALVIAARAPGVAGATFA
                     ALAVIAMAAVMGFVRTTR"
     gene            complement(10364..>11086)
                     /locus_tag="PKU75_10605"
     CDS             complement(10364..>11086)
                     /locus_tag="PKU75_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPF81373.1"
                     /translation="DWARSSRAGALQVAATHADLDTQILLIVDAFHDIGVTGGIAGVR
                     SSLDTAVRAATALAEHYLRTGERVGLIVLGSRDLPTVPATAGRAQVRRIRDVLAAIRP
                     GSVGVGDDRAVANALSRVPAGALVLVLTPGISAATLGRTHSLSRSGHSVLIIDTMPPA
                     AAEPLRLNATELDALAPMRSPRDAGVTRTAWRLRLLERATQLHRLVESGVPVVPWAGP
                     GSLDEVLRQLQRRARAPRLVRR"
BASE COUNT         1671 a         3896 c         3866 g         1653 t
ORIGIN      
        1 cgagggcggc acgactgcca ggctcgatca gggccaggac gtcggctagt ccgagggcgc
       61 gatacacgat cagggcgctg tagaacgggc cgacagccac aggtagtcga cgaccaactg
      121 cacgggccgg ccctgcgcgt cggcacccca ataggccgga taggcgccgt cgccccaccc
      181 cgatgacgag acggcgccga tcggtggccg gccgtcgaaa gcgagcaaca ggccgctgtc
      241 ctgggcgggg ttgagggtga agtcgtcgta cgcgcgctcc ttgtcgcgca cgctcgctcc
      301 ggcgtatgtc ggcaggtcca gcaccgcgat gttgccggcg tcgacgcccg cgacgtgccc
      361 gccgtccggg ctcaccgcgg gatgccagct gaccggctca ccgggcccga ggatgatgcg
      421 catcgccgcg caccggtcgt aggcgatcgc gacatccacg gcatacgacc cgggcgtcac
      481 ccgccgctcc agcggctcca gcttcgtcgc ttcgtacccg aagtcgacca ccgtcagggg
      541 cgacgggacc acgacagatc atccgacccc ccgtccagtc gcaggctgaa agacccgcgg
      601 cccggatcac ccgcgctcgg tgtcgttccg acgcgacggc attggcgacc cgaccgtcgg
      661 gagtgacaac tcgacgtagg atcgggcgct tgtgagcagg atcggggaga cggttgcgcc
      721 ggcgcggatg gggcgtgact tccgctggct gctcgcctcc tcctgggtcg gccagatcgg
      781 tgacgggatc gccctggccg ccgggccctt gttggtcgcc agccagaccc gcaacgccgt
      841 cctcgtcggg ctcgccgctg ccctgcagcg ggtcccgacc ctgctcttcg gtctcgtcgc
      901 cggcgcggtc gccgatcggc atgaccgcaa gcgcctggtc gtgctcagca acgccgcccg
      961 gatggccgtg ctcggcgttc tcgtcgcgac gctcgcgacg gggcacgtca atatcgccgt
     1021 ggtcctcgcc gcgatgttcg ccctggggtg cgccgagatc ttcgccgaca ccgcgttcgg
     1081 gacgatcatg ccgatgctcg tcgccaagga ggatctcggg ctcggccagt cccggctcat
     1141 ggccggcttc ctgaccggca atcagctggt cgggccgccc gtgggagcct tcctcttcgc
     1201 cgcggggatg gcgtggccgt tcgtcgtcca gatcgtcacc ttgcttctcg gcctgctcct
     1261 catcgtgcgc atcgcgacga cgacgcacgc gcgtcccgat cgcgaaccga cccacgtccg
     1321 ccgcgatatc gccgacgggg tgcgctggct gttgggcaac gctcccgtgc gcacgctcgc
     1381 cctggtcatc ctggtcttca atatcacctg gggcgcggcg tggtcggtgc tcgtgctcta
     1441 ctccctcgac cacctccaca tgggcgaggt gggcttcggg ctgctcacca cggccgccgc
     1501 ggccggcggg atcgccggca ccacgtcata cgggtggctc gagaggcact tcgccctcgc
     1561 cgccctgatg cgtgcgtgcc tgctgttgga ggtggccacc cacctcgccc tcgccatgac
     1621 gacctcgggg acggtcgcga tggggatcat ggtcgtgttc gggacgtatg cgttcgtttg
     1681 ggggaccttg tccaacgccg tccggcagcg ggccaccccg accgagttcc agggccgcgt
     1741 gtcgagcgtc tacctcgtcg gtgtcttcgg cgggctcgtg gtggggcaac tcctcggcgg
     1801 ggtgatcgcg caacggtggg gcctgaccgc gccgttctgg ttcgccggcg tcggatccgg
     1861 cctgacgctg ctcgtcgtat ggcgcgaact cgcccacatc gcgcacgcgg actctgcgtc
     1921 cgattaggga agctgcagct cgacgccgag ccactcggcg agccccgcca gttcgcgccg
     1981 tatgccgcca cgcgccgcac ccggccaggg cgcgtcctcg tggatcgcgt tgactcgcaa
     2041 cactcctcgc gtccggtcgg tctgcgcgtc gaccttgccg atgaggcggt cgccatgcag
     2101 gaccggcatc gcccagtagc cccagcgccg cttcgcggcg ggcttgtaca tctcgagctg
     2161 gtagtcgaag ccgaacagct ccagcgtgcg cttgcggtcg aacacgagcc ggtcgagcgg
     2221 ggagaggaga gccgtgcggc cgccgaacga ctgaccgagt tggccggggt cgacccgcca
     2281 ggtgcccttg acgccctcga tgaccgccgg ctccccggcg gccccggcgc tgctgacatc
     2341 ctcgaagacc tcgggcaccg tcgcccgcgc cacgccgagc gcggtcagcc ggcgctcgtc
     2401 ccggatccgc agcgcttcct cgagcggcac tgtgggcgag tcggggtaga cccgctcggc
     2461 gagatcccat tcccggccgt cgggcccgcg gccggcgacg gccacctcac cgcgccgggc
     2521 catcatctcc agcaggcgca tggtgttctt gtcgttggtc cagccggagg acctccacgg
     2581 caccagagtt acgtcgggca gcgcgcgggc gggcagcggg ccctcgacct ccagctcgtg
     2641 gcggatggcc cggcgcccct cgtcgttgac ggcgacccac tcggcgagat cctgctgcca
     2701 gggccgctcg ccacccggcc aggcggccat ctccgcgcga tacagcgcaa tgtcctcacc
     2761 cggccgcagc atcccgccga tctcgatgag cgagccgtcg gcgaggcgcc gatccacctc
     2821 tgccacatcg aactgcgcgc cgagccggct ccagagcacg agctcgtggc tcggtgccac
     2881 gtgccggcag ttgtcgacct ggaccgcggc caggtgccgc acgacctcca gcagggacgt
     2941 cggccgcgac cgggccagca actgggcccg gacgacgatg cggcgcgcct ggacggcggt
     3001 caggcgatgc ggctcgagcg ccatgtcacc cgcccgaggt gagcgcggca taggcgatgt
     3061 cgacgatatc gtcgcgggcg accgcctcct ccacactctc gtcgaactcg accccgccgc
     3121 cgggcatcga ccacagtccc tcggcacgcc cttcgccgtt ccaccacgtc aacaggatcc
     3181 ggtcgtctgc gtcgacgagc acggcgtagg ccgcgacccg ggtgtcgtat tgggtgtagt
     3241 gcacggcagt tcactgcgcg atgagcggag cgagggtctg gccgagcagg gcgacgcggc
     3301 cggggacgtg tccgttggcc ggcatattga ccgtcacccc gtccacgccg gtgtcgagga
     3361 tcttcgcgta tgccgcggcc acctcctcgg ggccccccac gatggcgctg ccacgccgca
     3421 tacccaactc gggggtgcgg gcgacggccg cctcgaagtc cgcctcggcc tcctcgcgcg
     3481 tgggggcgac gacgcatgag gtctgatagg tcatggtgat ctcggcacga tccctgccaa
     3541 ggcgatcgca gtgtccggca agggcttcca gcttgcgtgg aatgtcctcg acgggacaga
     3601 tgatgttgga ctcgtcggca tactgggcca ccatgcgcag cgtcttcttc tcgccgccgc
     3661 cgccgatcat caccgggatg cgtccgacgg ggccggggga gttgatggcg tccgtgacct
     3721 gataccacct gccgtcgagg gtcggccgct cgccgcgcaa catcgggagg atgatctgca
     3781 gggcctcctc gagcttctgg aagcgatcgg tgaaggtgcc gaactcgtag ccgagggcgt
     3841 cgtgctccag ttcgaaccag ccggcgccga tcccgagctg cgcccggccg tgcgagacga
     3901 cgtcgagcgc tgtcacgatc ttggcgagca gggcggggtt gcggtaggtg ttgccggtca
     3961 cgagcgcgga caggcgaacc gtctcggtgt gctgggcgag ggccgagagg agggtgtagc
     4021 actcgagcat ctcattggcc ggcgacccga gcatcggcag ttgatagaag tggtccatca
     4081 ccaggaccgt gtcgaagccg ctcgcctcgg cctccttggc ctgcgcgacg atgacatcga
     4141 agaggccgcc gctgtcgacg cccgggtagg tgaagttcgg gatctgatag ccgaggcgcg
     4201 tcatgccacc aacgtacgcg gctcacccga acgccgcgcg cggattcggc tgtgagcgga
     4261 accgggcgcc acgggtacga catgtccggt tcaatggtcg tgtcgggccc cgacggcagg
     4321 tagcgtcggg cccggaggcg atcgacccgc gggtttcgcc ggacggcagc gcaggaaggg
     4381 acgaggcaga cacatggcac gcgttggtga gagcagcgac ctgttgaaat gctcgttctg
     4441 cggcaagagc caaaaacagg tcaagaagct gatcgccggc ccgggtgtct acatctgcga
     4501 cgaatgcatc gacctgtgca acgagatcat cgaagaggag ttggccgagt cgaccgatct
     4561 cggcttcgac gaactgccca agccccgcga gatcttcgac ttcctgcaga cctacatcat
     4621 cggccaggag ccggccaaga aagccctggc cgtcgccgtc tacaaccact acaagcgcat
     4681 ccaggcgggc gaggccgcca acaagcgcga cgcgaaccac gtcgagatcg ccaagtccaa
     4741 catcctgctc atcgggccga ccggctgcgg caagacctat ctcgcccaga ccctcgccaa
     4801 gatgctcaac gtcccgttcg ccatcgccga cgcgaccgcg ctgaccgagg ccggctatgt
     4861 cggcgaggac gtcgagaaca tcctcctcaa gctgatccag gctgctgact acgacgtcaa
     4921 gaaggccgag acgggcatca tctacatcga cgagatcgac aagatcgccc gcaagagcga
     4981 gaacccgtcg atcacccgcg acgtctccgg tgagggggtc cagcaggcgt tgctgaagat
     5041 cctcgaaggc acgaccgcca gcgtgccgcc gcagggcggg cgcaagcacc cgcaccagga
     5101 gttcatccag atcgacacga ccaatgtgct cttcatcgtc ggcggagcct tcgccggcct
     5161 ggagaagctc atcgagtccc gcaacggcaa gaagggcctc gggttcggct ccgagctgca
     5221 tacgcccaag gatctcgccg agtcgatcgt cgacgtcatg cccgaggacc tgatgaagtt
     5281 cgggctcatc cccgagttca tcggccgcct gcccgtcatc acgaccgtca gcccgctcga
     5341 cggtgacgct ctcgtgcgca tcctgtccga gccgaagaac gccttggtca agcagtacgc
     5401 caagatgttc gaactcgacg gggtcgagtt ggagttcacc tccgaggcgt tgaccgccat
     5461 cgccgagcag gcgctcaagc gcggcacggg cgcccgtggg ctgcgcgcca tcatggagga
     5521 agtcctcatg ccggtgatgt tcgacgtgcc gagcgatgac gacatcgccc gcgtcgtcgt
     5581 caccgaggag accgtgctcg gcaacgtcct gccgaccatc gtccggcagg agcacgagcc
     5641 cccgacccgc aagcgcagcc cgcgcaagcg ctctgcctga ccgggcccca ccccgcggtt
     5701 ggcagggcct ccccgacccg caaggatggg gccatgacgg cgcccttccg gctgcatgct
     5761 ggcgacagcg caccgagcca gcagagtccc acgacgtgtg gcgccgcctg cctgaccgtc
     5821 gctcgcatgc tcatcgaccc gctgttcgct cgctggatcc tcaccggtga cgggccgcgt
     5881 cgcggcgtcc ccgacgcccc gacgctgggg gagcgcttcg ccgcctacga gcggatcgtg
     5941 caccgccgga ccaacgggct ctatgccggg gccggccggc tcaacctgcc ctggccgcgc
     6001 cgcctgggca ccccgccgtg gggcgcgaag aaggaactcg aattcggtgc cgcccgccgc
     6061 gacaccgact acaccgtcga gatgctgcgc caccacagcc ccgccgagtt gggcgagcac
     6121 ttcgaccgct tactcgccat cgtcgccgac ggcgagccgg ccctgctgta tgtcgggacc
     6181 cacctcgtcc cgcgccacgt cctgctcatc cttccggaca acggcgacgg caatctggag
     6241 gtgtacgacc ccgctcgcgg gacggtcacc gaactgaccc ggcacgcgtt caccagccga
     6301 gccgtggacc tggccggctg ggacgtgccg tggttcagcg tcgaaccggt cgggatcgtg
     6361 cgcgaacgcg cctacgagtg ggcccccggc gccgtccccg cctgagcgag ttagtcagcc
     6421 agtagcgaaa accctttgat agtgcgccag ttcgcgggtc atcgcatcct cgatggtcgt
     6481 cgcggcgcgg aagccgtggc cttcgccggg gtagatgacc agctcgacgt cgcggccggc
     6541 cgcgcgcatg gcgtcggcca tctgctgggt ctgggcgagg gggacgacca tgtcgtccgc
     6601 gccgtgcagc agcagcagct cgccgtgaat ctcgtcgaca tggttgatcg gggagcggtc
     6661 ggcatagacg tccgccgctt ccggccacgg acccaccagt ccgagcgtgt agcgggattc
     6721 gaacttgtgg tcgtcgttga gcaacacctt caaatcgctg atgccgaaat agcttgtgcc
     6781 ggcggcgaat acgtcgccgg acgtcagcgc gcgcagcgtg ctgtatccgc cggcgctgcc
     6841 gccccggatc gccaggcgcg cgctgtcggc ccggccctgc gcagccatgg cggccgcgcc
     6901 ggtgacgcaa tcgtcgatgt cgacgacgcc ccactgtcca cgcaaccgct cacggtaatc
     6961 tcgcccgtgc ccggtggagc cgccgtggtt gacgtcgagg atcgcgaagc cgcgcgtcgt
     7021 ccagtactgc agcccggtct gatacgacgg aagcgcggcg ctggtcggcc cgccatgaac
     7081 gaacaccaga agcggcggga gatcgccgtc ggggccggtg tggccggcgt tgaccggcgg
     7141 atagaaattg ccgtatgccg tcaggccggc gctgttggtc caactcacct cctccgcccg
     7201 ggagacgtat cccgggtcgg gcacgtccgg tgacgaggtg gccagcacgg tcacgtcgcc
     7261 ggtgcccgtc tcgacggaca cgacctcggg gagccggtcg gcgagcccgc gtttgagggc
     7321 gacgcgatcg cctgtggcac agatggtctc ggtgtgcgtc gatgggacgc caagaggcgt
     7381 gagcgcgccc gtgtcgtcga ccagggtcag gccgtgcgcg ccgtcccgga tgacggcccg
     7441 gccatcatcg atgaaggcga tgtcccgcat tccgagcatc cactgcggac tcgccaggtc
     7501 ggcctcgacg cccagcacct ggcgcgtccg gccggcgtcg acgatccacg gcgtccacca
     7561 gttcgtctcg tcgctgaaga accacagccg cccggcgggg tcgtaggccg gctggctgac
     7621 cgagactccc gggcctccgg cgatcacggt ggcgtcgctc gcccccgcgc cggtcagact
     7681 cgcctcgtgc aaggtcgtgc tgtcccatgg catgttcggg tgatcccacg acacccatgc
     7741 cacccgggtt ccgtcgggag acaccgccgg ccgggacacg aagtcactcc ccgtggccag
     7801 gacggttccg aagtcggcgt tgtcgccgtg cgggtcgaga cggaccagtt cgttcaccgg
     7861 ctcgccgccg gcccggtggt cctcacggac ggcatacagc caatccccgt gcaggacaag
     7921 gccaccgaag cgaacctcgc cctcgggggt gatcgcgacc ggttcgaggg cacccgggtc
     7981 cagacgtcgc agccggccgt cgccgaagtg gctgaagacc acgatgccgg actgcacggc
     8041 atacgccccg ccgccgtatt cgtgcacgcg ggagcggacg ttccacggtg caagcgtgac
     8101 ctcgactcgc tcgcctgcgg cgccggcgtg gcgcatcagg acggtgcggc cctcctccca
     8161 gggccggccc tcgagccagt aggtgtccgc gccgtcgacg gcaacgtcgt cgaggcgggc
     8221 ttgcgccgcg gtcagcgagg cgggagtgat cggtgagggc caggtgccgt acgggaggac
     8281 tgtcacccga cgaagcctag gtcagcggct gtctccacgc tcggggtgga agtaccagtc
     8341 ggtgtcgccg cgtgcgggca gtcgggccgg gtagcacgtg gtgttcgtgg ggtaggcgcc
     8401 cgtgaagtac ttgtatgcgg atttgaaccg gtcgcagcgc cgggtcgcct cttcatagga
     8461 ccaggcgaaa ccgatattgc ggttgacacc gttcatgtcg tggtcgtcgg cgtaggcgtt
     8521 ggtcgtcgtg cgcgccacgg tccagtacca ccgggcgatc ggagcactgt aatgcagcga
     8581 cttcgcgaga tccggctgcc ccagcaggtt gatgccgaag taggtgccgg cggacgcgta
     8641 gttgaagtcg ttggtcagct ggatgtagcc gcgcccgcgg taggtctggg tgttgccgct
     8701 ctcgtcggcg tcgtagcgca tcgccgactc gctgacgagg gtggcgagga aggcggcctt
     8761 gcgcgccgga gtgttgatgg cgcccttggt catctcggcc tgcaacccgg gaagcccccg
     8821 ctcgacgatg tcgcgcgggc cgacccagcc ggagccgaac atcgtcacga gcccgtcata
     8881 ggtgatgccc cccgagggac agacgacatt gcgcggtccg gtggtcagca gcttctgcgt
     8941 ccagccaccg tctccgacat acccgatcag gctgatgtcg gtgccgctgc tgttggtgat
     9001 atcggcgtcc cggcgggtcg agagcacgcc cgacgagtcg gtgcgcgcga agaagaggcc
     9061 cccgccgggc gaggccagcg ccgacacccc gccccagccg ctgctgccca acgtcgagat
     9121 cgtcaggttg cccgctcccg ccgcgcggat gccgagcagt ttccccccgc tggtcgtcgc
     9181 gaggaggcga ccgccgccga cgccggtcag ggtcttggcg atcacgcccg cgccgatccg
     9241 gaccttctga ttgatctgcg agccggtggg tttcttggcg ctcacgtccc agcgccacag
     9301 ctggccgttg gtgtcaccgg tcgcgaacag gtggccggcg ccgtcggccg cgagcttgtc
     9361 gtagttccag ccggtatacg ccatcggcac cggggcaccg atcgtcagac tcttggtgtt
     9421 gccggtgacg tcgatccgat agagcgcgcc ctgactcgtg gtcaccagca gcgtgttcat
     9481 gttcagtgcg gtcatcgccc gcacccccca ggggagcttg ggtccggtga gcttgcgggt
     9541 gcgcacattg gtcgccgcgt cgatggcggc gaaggtcaag gtcgcgtccc gtcggacgcc
     9601 atagacgtat gccgtgccgg tgcacccgct ggcggcgcgc agcgcatcgt ccggggtgtc
     9661 gtcggggccg gcggccccgg tcgtggtggc gagggacaga ccgacggcga tggcggcggc
     9721 gctgccgatg aaggacgtcg tggacatgga acaacctctc cggacgcgaa aaaccgtgac
     9781 cattgtgccc taagtgacac gtgagaataa gcacggccat cccggatgcg tcagcgcgtc
     9841 gtgcgcacga atcccatcac ggccgccatc gcgatgacgg ccagtgcggc gaaggtcgcc
     9901 ccggcgacgc cgggggcgcg ggccgcgatg accagcgcgc cgagggcggc ggtgaccagg
     9961 atgccgaccg cgacgagccc ggcgtcgcgc gcccaccgca tacgggcctc ggcgggcacc
    10021 cgcaccccgg gcggccagac gccgcaggcc gaggcggcga ggtgcatcac gaggaggagc
    10081 agcgctgcac ccgccggaag catcaggcca gccggcgatt ccggccgggg cacgaacagc
    10141 caccacacgc cgcagtgcag cagcgccagg accgcggtga cccaggtctc cggcgccgcc
    10201 gccgcggcga tcgcggccag cacgagatag ggcacgaagg ccagatcccc gccgggggct
    10261 gcggcgctcg tgagcccggt ggtggcgagg ccggccagca ggatcagcag acgcagcagt
    10321 atgcggtcgc gaccctgccc cgcgaaccac tcccggaccg tcgtcatcgc cggaccagcc
    10381 tcggcgcgcg ggcccgccgc tgcagctgac gcaacacctc atcgagcgag ccggggccgg
    10441 cccaggggac cacgggcacg ccggactcga cgagccggtg cagttgcgtc gcgcgttcga
    10501 gcagccgcaa ccgccacgcc gtgcgcgtca ccccggcgtc gcggggcgag cgcatcggcg
    10561 ccagcgcatc gagctcggtt gcgttcaggc gcaacggttc cgccgccgcc gggggcatgg
    10621 tgtcgatgat caggaccgaa tggccggagc ggctcaggga gtgggtccgc ccgagcgtgg
    10681 ccgcggagat gcccggagtg agtaccagga cgagggcacc agcggggacc ctcgagaggg
    10741 cgttggccac ggcccggtcg tcgccgaccc cgacgctgcc gggccggatg gcggcgagca
    10801 cgtcgcggat ccggcggacc tgggcgcgcc cggcggtggc cggcacggtc ggcaggtcgc
    10861 gggacccgag cacgatcagg ccgactcgct cgccggtgcg cagatagtgc tcggccagcg
    10921 cggtggccgc tcgcaccgcc gtgtccaggc tgctgcgcac gccggcgatg ccgccggtga
    10981 cgccgatgtc gtggaaggcg tcgacgatga gcaggatctg ggtgtcgagg tcggcgtggg
    11041 tcgccgcgac ctggagggcc ccggcgcggc tcgaccgggc ccagtc
//
LOCUS       DAOPJG010000110        11085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6185_length_11085_cov_8.250861, whole genome shotgun sequence.
ACCESSION   DAOPJG010000110 DAOPJG010000000
VERSION     DAOPJG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 11085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11085
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6185_length_11085_cov_8.250861"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..372)
                     /locus_tag="PKU75_10610"
     CDS             complement(88..372)
                     /locus_tag="PKU75_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019175985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPF81374.1"
                     /translation="MAAQASPYRVELTARARKQLKKMDRFDAKILATWIKNNLDGCAD
                     PRAFGKGLTANHSGEWRYRVGSYRILALIHDDIVRIEVFSIGRREKVYDR"
     gene            complement(372..584)
                     /locus_tag="PKU75_10615"
     CDS             complement(372..584)
                     /locus_tag="PKU75_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019175984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HPF81375.1"
                     /translation="MSTMTVRMNDQDAELVRKFAQFEGVSISDFARTAILEKIEDSYD
                     LQELRAAIAQDTGERFGIDEILTELA"
     gene            1048..1251
                     /locus_tag="PKU75_10620"
     CDS             1048..1251
                     /locus_tag="PKU75_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HPF81376.1"
                     /translation="MGVPPRRINEIVHGKRGITADTAVRLAKYFGNSAEFWMNLQSHY
                     ELRIERRALRAQVDAIQPIEHAS"
     gene            complement(1875..2555)
                     /locus_tag="PKU75_10625"
     CDS             complement(1875..2555)
                     /locus_tag="PKU75_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HPF81377.1"
                     /translation="MNSREAILARIREANRRVESEREALARLRPTAPSEAFDAWEYGR
                     PTAMPDVLGRFVERVEDYRAVVVRVPEPDIAEAIGRALGDHGADSVVVPSGIPTPWLS
                     TLPGTVRVHHDDPPLSHTELDTTSAVVTDSAVGIAETGTIVLDHGPGQGRRALSLVPD
                     LHICLVRADQVVSDVPEAVARLAEAVRAGRPLTWISGPSATSDIELSRVEGVHGPRTL
                     HVILVDSP"
     gene            complement(2552..3985)
                     /locus_tag="PKU75_10630"
     CDS             complement(2552..3985)
                     /locus_tag="PKU75_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HPF81378.1"
                     /translation="MPAFRHGAKNALANTQQRRNLRHATGTIRAKRAAVVGEVADWEG
                     LRRAGEDVKNDVLAHLDSYLVQFEEQFTKAGGTVHWASDADEANRIVTDLVRATGADE
                     VVKVKSMATQEIDLNEALEDAGIAAWETDLAELIVQLGHDRPSHILVPAIHRNRSEVR
                     EIFLEEMGRYGTAAPGDISDSPPELAGAARIHLREKFLRAKVAISGANFAVAETGTLV
                     VVESEGNGRMCLTLPETLISVVGIEKVLPSVADLEIFLQLLPRSSTGERMNPYTSMWT
                     GAGSGDGPQDVHVVLIDNGRTNALSRLHGRSALRCIRCSACLNICPVYERVGGHAYGS
                     VYPGPIGAVLTPQLRGITSDIDASLPYASSLCGACFEVCPVRIDIPELLVQLRHDVVE
                     KKSHARVPGANQAIFAGASWMMADARRLTAAQRLASAAGRVLPKSGHLGPLPWPMSKW
                     SNARDLPNPPAESFRDWWKRERGGEPG"
     gene            complement(4066..4884)
                     /locus_tag="PKU75_10635"
     CDS             complement(4066..4884)
                     /locus_tag="PKU75_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPF81379.1"
                     /translation="MTGENLEASVRPTAITDPGAGRPGAGKTVALFATCINDTMFPQT
                     PKAVVSLLERLGCRVEFPLGQTCCGQMFTNTGFFDEALPTVRRYAEVFAPYDYIVAPS
                     GSCVGSVRDQHPMLAERTGDRSLVERVRAVTARTLDLSEFLVDVLGVTDVGAYFPHRV
                     TYHPTCHSVRVAKVGDRPYRLLQEVRGITVVPLPAADQCCGFGGTFSVKNPDVSVAMG
                     ADKAHHVMETGAEFVVTGDNSCLMHIGGVLSRLNSGIRPIHLAEILASTEGAHP"
     gene            complement(4881..6584)
                     /locus_tag="PKU75_10640"
     CDS             complement(4881..6584)
                     /locus_tag="PKU75_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HPF81380.1"
                     /translation="MTAAYTPNPTALGNSLWLTALIALLPLVTIFVTLGALRWKAHKA
                     GLTSLAVAILVAILAFRMPVDLALLSATEGAVFGLFPITWIVMTAIWLYQVTVVSGRF
                     EDLRETFNLVSDDPRVTAILIAFCFGGLMEALAGFGAPVAITGVMLMAVGFSALRAAM
                     IVLLANTAPVAFGAIAIPIITAGNLTGIDYHDIGAYVGRQTPLLAAFVPLLLVFLADG
                     RRGLRQTWPVALTVGVSFGVAQFISSNYVSVELTDIIASLVGLAAGVLLLQVWKPAGR
                     EEANERLRTELEREHAELGLTDPDAGGAGGVSTMTKIAVAQPITGSRIGMALFPYLLV
                     IAVFSLAKLWGPLKDFLAGTDRKIHWPGLDGEILNAAGKPVSSTVYNFQWLSSPGTLL
                     LICGFIIALVYRLPMRKAIGEFAATVHKMRFPMLTIASVLALAYVMNLSGQTITIGTW
                     IAGTGAAFAFLSPILGWIGTAVTGSDTSANALFATLQQTAGAKAGIDPTLLVAANTSG
                     GVVGKMISPQNLTIAATAVGLLGQESLIFRKVVAWSIGLLVILCLLVGLQSTVLSGML
                     P"
     gene            complement(6581..9484)
                     /locus_tag="PKU75_10645"
     CDS             complement(6581..9484)
                     /locus_tag="PKU75_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HPF81381.1"
                     /translation="MLHSETGPTQDLIARLQAAVGEKRVADSQLDRSRMAHDASHYLL
                     QPQAVVFARDGADVAAAVAAAAQAGASVTFRSGGTSLSGQAVTDGVMIDTRRGFRDVE
                     VLDGGARVRCQPGATVRAVNARLTPYGRLLGPDPASEIACTIGGVVANNSSGMTCGTT
                     ANTYRTLSSIQVVLVTGTHIDTGAPDADEALRALEPDLYAGLLRLRDRVRGNAASVDR
                     IRHQFSMKNTMGYGLNAFLDFDTPAQILAHLMVGSEGTLGFIVEATFDTVPLHAHAGT
                     AFLVFDDISHATDALPALVAAGVATAELLDASSLRVAQDLPVTVPALRDLAVARHTAL
                     LVETQADDPEELRERVARLGGVVAGLGGLTDLGYPVEFTSDPGERAVRWQVRKGLYTA
                     VAGARPTGSTALLEDVVVPMPALTGLVGDLQELCSRHGYGDTVIFGHAKDANLHFMIN
                     PDLRDPVQLERYAAFSDDLVDLVLAADGSLKAEHGTGRIMAPYVERQCGPELYAVIRE
                     IKRLFDPAHVLNPGVIVTDDRDQHLKNLKIPEVVDAAVDRCVECGYCEPACPSKDLTT
                     TPRQRIALMRELAAAEGARREELARDFTYAAVETCAADSLCLLSCPVGIDTGKVMKRH
                     RHDAQPAAAQRIAAGLATHWDATLAALRSGIALAEHIPAGLLTTATEAGRAIASSDLV
                     PRVGDDLPGPGISRRTPARHEPGDVVFFASCMGELFAPAASPGATTRPRGAAFAFLRL
                     CDAAGVRVQLPVGLETLCCGTVWRSKGLTDGLRTMAIATAVALLSASRDGEVPVVSEA
                     SSCTHGLHSLAEDVAAAGEAELASRVSRLDIVDSTRFVAERVLPSLTVPRQLGSVMVH
                     PTCSDRHAGDVPHLLAIAQACAERVVVPDDAGCCAFAGDRGLLHPELTASATRREAAE
                     VGRAAYDAFVSSNRTCELGMTRATGQPYRHVLELLDDLVAESRSPIPEGGRSS"
     gene            9660..10358
                     /locus_tag="PKU75_10650"
     CDS             9660..10358
                     /locus_tag="PKU75_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPF81382.1"
                     /translation="MPRSRTYTYVIDQVDEQIVSGALRVGDRLPPEREFATLLGVGRG
                     AVREAIRVLEAYGVVRTDPGPEGGTTLTSMPSEALTQVLRLHIGLANFPMADVIEARA
                     MLERWSARLAARHGSADDLAAIEHELLQMENGRSDREAFNEHDTAFHVAIARASNNRL
                     VADMTTALRDSIRSLVLESFHGHADWPALAEQLCADHRRVFEAIASGDESAAADAVEE
                     HIRSAYATLGWGRA"
     gene            complement(10389..10667)
                     /locus_tag="PKU75_10655"
     CDS             complement(10389..10667)
                     /locus_tag="PKU75_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81383.1"
                     /translation="MRHRDPFLVVAAALIGAGLLLSALKMSTSLAHPEGATPAAADLV
                     YLVPYAATAGLVLLLRRSFRRGSVSQERLLSSLVGGTDRRQVREPVDR"
     gene            10823..>11085
                     /locus_tag="PKU75_10660"
     CDS             10823..>11085
                     /locus_tag="PKU75_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81384.1"
                     /translation="MTLLAGWLSAEAVHALAARAVLPARRPAPGEGGLAVVVLGYHNE
                     DPAAINWLNRWRADVAVRSIPRDGQAPLIVASGGSRRPGLSEAA"
BASE COUNT         1813 a         3616 c         3910 g         1746 t
ORIGIN      
        1 tgatcaaccg ggcttctgcg gtgagctcgt catgctcaac gagttcggaa ctcggtggag
       61 ttcaggttct atgtgtaacg aacagagtca gcggtcgtag accttctcgc gccgtccgat
      121 cgagaagacc tcgatcctga cgatgtcgtc gtggatgagt gcgaggatcc ggtaggagcc
      181 cactcggtag cgccattcgc ctgagtggtt tgccgtgagc cctttgccga aggctcgtgg
      241 gtcggcgcat ccgtcgaggt tgttcttgat ccacgtggcg aggatcttgg catcgaaccg
      301 gtccatcttc ttcaactgct tgcgcgctct cgcggtcaac tcgacgcggt atgggctggc
      361 ctgtgcggcc atcaggcgag ttcggtgagg atctcgtcga tgccgaagcg ctcgccggtg
      421 tcttgcgcga tcgccgcgcg cagttcctgc aggtcgtagg agtcctcgat cttctcaagg
      481 atggcggtgc gggcgaagtc ggagatgctg acgccctcga actgggcgaa cttgcggacg
      541 agttcagcgt cctgatcgtt catgcgcact gtcatggtgc tcatgggtcc tcctctcacg
      601 gttctgaata cagtgtattc agggtggtgg gtaggggcaa ggtgaagcgc tgcggtcggc
      661 cgagtatgga ttcagcgtga acggcgatcg gcgggagtga ggggctcacg aaccgtccgg
      721 cacgagccgg cgttccgtca gaattggtac cgcgttttcg tcagtctggc cagcccaacc
      781 gtgagccatc gccatccgca gacccgacgg accttatggg ccgagtagtc accgggggag
      841 acccgaccgt gcatggactt tcctcgagga tgcgaacggc tggctggaag actacgcgaa
      901 ccgcctggtg gagcggggcg ccgccgacgt cgcaccggcg caagctccgg tgcttcgcaa
      961 gctgctgttg ctgcttgccg ccgcgccagg ggccgagctg gttcaggatc gtttggcgca
     1021 cgaagctggg cgtggccagc ggtcgccatc ggtgtgccgc cgcggcggat caacgagatc
     1081 gttcacggca agcgggggat cacggccgac acggccgtgc ggctcgccaa gtatttcggg
     1141 aactcggccg aattctggat gaacctccag tcacactacg agcttcggat cgagcgacgg
     1201 gcgctccgtg cgcaggttga cgcgatccag ccgatcgagc acgcgtcatg atcatgactt
     1261 tgccgtgcca ccgactacac aaagccccga tctttgagca ctgcaagcgg ggcgggggcg
     1321 tgagaatcaa cttcgtgtag tcgcaggacc gaagtgctat cgaggtggtc cccgtgaccc
     1381 ggctcgagct gggcggttta cggtatggcg agtaccaacc cgggaggtag gcagtgagtc
     1441 gtcggtcttg ggcacgcgcc gcgttgatgg cggacggcgt cgggtgcgct ttggccgcgg
     1501 tcggcgccct cgccatcggg cgcctcgggg atgagctgga ccccatctca tcggcccgga
     1561 tcccgctcgg ggccgcgctt gcagtcacga gcgcgatgct tctcggttca gctcgtgacg
     1621 cccgcgaccg agacctcagc cgtgccgccc tggtgaatac cgcgtgggct ctcgggtgca
     1681 cggccgccct gacggcgagg catacgcgga cgagccgtct cctcgtcgcc acgacggcgg
     1741 tcgccgatgc cgcgatggcc gtcactcagt ggagcctgcg atcggaccga cacggggtcg
     1801 gattcaggcg ggccgacgcg tgatggcaac gcgtgcccgg tcgctcggcg gcggatggtg
     1861 cgcggatggt gcgatcaggg actgtccacg aggatgacgt gcagcgtgcg tggtccgtgg
     1921 acgccttcga cccggctcag ctcgatgtcg ctggtcgcgc tgggaccgct gatccacgtc
     1981 agcggccggc cggcgcgcac cgcctcggcc agccgggcaa cggcctccgg cacgtcactg
     2041 accacctgat cggcccgcac gagacagatg tgcaggtcgg gaaccaggct gagcgcccgc
     2101 cgcccttgcc cgggcccgtg atcgagcacg atcgtcccgg tctcggcgat gccgacggcg
     2161 gagtcggtga cgaccgccga ggtcgtgtcg agttcggtgt gcgagagcgg cggatcgtca
     2221 tggtgcaccc ggacggtgcc gggaagggtc gacaaccacg gagtgggtat gccgctcggc
     2281 acgacgacgc tgtcggctcc gtgatcgccc agggcgcgtc cgatcgcctc ggcgatgtcc
     2341 ggttcgggca cgcggacgac gaccgcgcgg tagtcctcga cccgctccac gaacctgccg
     2401 aggacgtcgg gcatggcggt cggccggcca tactcccacg catcgaatgc ctcggacggc
     2461 gcggtcggcc gcagtctggc cagcgcctcc cgctccgact ccactcggcg gttcgcctcg
     2521 cggatccggg ccaggatcgc ctcgcggctg ttcatcccgg ctcgcccccg cgctcgcgct
     2581 tccaccagtc ccggaacgat tccgccggcg gattcggcag gtcacgggcg ttggaccact
     2641 tcgacatcgg ccacggcagc gggcccagat gccccgactt cggcagcaca cggccggccg
     2701 ccgacgcgag cctctgggca gcagtgaggc gtctggcgtc ggccatcatc caactcgccc
     2761 cggcgaagat cgcctggttg gcacccggca cgcgggcgtg ggacttcttc tccacgacat
     2821 cgtgtcgcag ttggaccagc agctcgggga tgtcgatgcg gaccgggcac acctcgaagc
     2881 aggccccgca caggctcgac gcgtagggca gcgaggcgtc gatatcgctc gtgatgccac
     2941 gcaactgagg cgtcagcacg gcaccgatcg ggcccggata gaccgagccg taggcgtggc
     3001 cgccgactcg ctcatacacc gggcagatgt tcaggcaggc cgagcatcgt atgcagcgca
     3061 gcgcggaccg gccgtggagg cggctcaagg cgttggtccg cccgttgtcg atgagcacga
     3121 cgtggacgtc ctgcgggccg tcgccgctgc cagctccggt ccacatcgag gtgtaggggt
     3181 tcatccgttc gccggtgctg ctgcgcggca acagttgcag gaagatctcc aggtcggcca
     3241 ccgacggcag gaccttctcg atcccgacga ccgagatcag ggtctcgggc aaggtcaggc
     3301 acatccgccc attgccctcg gactccacga cgaccagcgt gccggtctcg gccactgcga
     3361 aattcgcgcc ggagatggcg accttcgcgc gaaggaactt ctcgcgcaag tggattcgtg
     3421 ccgcacctgc gagctcgggc ggggaatcgg aaatgtcgcc gggcgctgcg gtgccatagc
     3481 gacccatctc ctccagaaag atctcgcgca cctcggaccg gttgcggtgg atcgccggga
     3541 cgaggatgtg gctgggtcgg tcgtggccga gctggacgat caactcggcg aggtcggtct
     3601 cccatgccgc aatcccggcg tcctcaagtg cctcgttaag atcgatctcc tgggtggcca
     3661 tcgacttgac cttgaccacc tcgtccgcgc cggtggcgcg gaccagatcg gtgacgatcc
     3721 ggttggcctc gtcggcatcg ctggcccagt gcacggtgcc gccggccttg gtgaactgct
     3781 cctcgaactg cacgagatag ctgtcgaggt gggctagcac gtcattcttg acgtcttcac
     3841 cggcgcgccg cagtccctcc cagtcggcga cctcgccgac gacggcggct cgcttggcgc
     3901 ggatcgtccc tgtcgcatgg cgcaggttgc gtcgttgctg ggtgttggcg agcgcgttct
     3961 tggcgccgtg acggaaggcc ggcatcccga ggaaggtctc ctcaggcggg gccggggtgc
     4021 ggcgcttacg cgcgacggcg acggtcggct gcttgggcac gcgggtcatg ggtgcgctcc
     4081 ttcggtggac gcgaggatct cggcgaggtg gatcggccga atccccgagt tgagtcggct
     4141 cagcacccct ccgatgtgca tcaggcacga gttgtcgccg gtgacaacga attcggcgcc
     4201 tgtctccatc acgtgatggg ccttgtccgc gcccatcgct accgagacgt ccgggttctt
     4261 gaccgagaag gtgccgccga aaccgcagca ctggtcggcc gccggcaggg ggacgaccgt
     4321 gatgccgcga acttcctgca ggaggcgata tggccggtcg cccaccttgg ccacccgaac
     4381 ggagtggcac gtcgggtgat atgtcacccg atgcgggaag taggccccga catcggtgac
     4441 gccgaggaca tccacgagga actcgctgag gtcgagggtc cgggcggtca ctgcgcgcac
     4501 gcgttcaacc agcgagcgat ccccggtgcg ctccgcgagc atcgggtgct ggtcgcggac
     4561 ggacccgaca cacgagcccg acggcgcgac gatgtagtcg tatggcgcga acacctccgc
     4621 ataccggcgc accgtcggca gggcctcgtc gaagaacccg gtgttggtga acatctggcc
     4681 gcaacaggtc tgtcccagag ggaattccac gcggcacccg agtcgctcga gtagcgagac
     4741 caccgccttg ggcgtctgcg ggaacatcgt gtcgttgatg catgtggcga acagcgcgac
     4801 ggtcttcccc gctccaggac gtcccgcccc agggtcggtg atcgccgttg ggcgcacgct
     4861 cgcctccagg ttctcgcctg tcatggcaac atccccgaca gcacggtcga ctgcaggccg
     4921 accagcaggc agaggatgac cagcaacccg atgctccacg caacgacctt acggaagatc
     4981 agcgactcct ggccgagcag gcccacggct gtggcggcga tggtcaggtt ctgggggctg
     5041 atcatcttgc cgacgacacc accggaggtg ttcgccgcca cgagcagggt cggatcgatt
     5101 ccggccttgg cgccggccgt ctgctgcaag gtggcgaaga gcgcgttcgc gctcgtgtcg
     5161 gaaccggtca ccgcggtgcc gatccagccg aggatggggg agaggaaggc gaatgccgcg
     5221 ccggtcccgg cgatccaggt gccgatcgtg atcgtctgcc cggagagatt catgacatag
     5281 gcaagagcaa ggaccgaagc gatcgtcagc atcgggaaac gcatcttgtg caccgtggcc
     5341 gcgaattccc cgatcgcctt gcgcatcggg agccggtaga caagagcgat gatgaacccg
     5401 cagatcagca gcagggtgcc cggcgaggag agccactgga agttgtagac cgtggagctg
     5461 accggtttgc cggctgcgtt gaggatctcc ccgtccaggc ccggccagtg gatcttgcgg
     5521 tcggtcccgg ccaggaaatc cttgagagga ccccagagct tggccaggga gaagaccgcg
     5581 atcacaagca ggtaggggaa caacgccatt ccgattcgag agcccgtgat cggttgtgca
     5641 acagcgattt tcgtcatcgt cgacacgccg ccggcgccgc cggcatccgg atccgtcagg
     5701 ccgagttcgg cgtgctcacg ttcgagctcg gtgcgcaggc gctcattggc ttcctcccgg
     5761 ccggcgggct tccagacctg gagcagcaac accccggccg cgaggccgac gagggaggcg
     5821 atgatgtcgg tgagctcgac ggagacgtag ttggaactga tgaactgcgc gaccccgaag
     5881 gacacgccca ccgtcagggc caccggccac gtctgccgca agccgcgcct gccgtcggca
     5941 aggaaaacga gaagcagggg gacgaatgcg gcgagcaacg gggtctgccg gccgacatac
     6001 gccccgatgt cgtggtagtc gatccccgtc aggttgccgg ccgtgatgat cgggattgcg
     6061 atcgcaccga aggcgaccgg ggcggtgttt gcgagcagga cgatcatcgc tgcccgcagc
     6121 gctgagaagc cgacggccat cagcatcaca ccggtgatgg ccaccggcgc gccgaagccg
     6181 gcgagggcct ccatcagccc cccgaagcag aaggcgatga ggatcgccgt gacgcgcgga
     6241 tcgtccgaca cgaggttgaa cgtctcgcgc aggtcctcga agcgtccgct gaccaccgtc
     6301 acctgataga gccagatcgc ggtcatcacg atccaggtga tggggaagag gccgaagacg
     6361 gcgccctccg tcgctgacag cagcgccagg tcgacgggca tccggaatgc aaggatggcc
     6421 acgaggatgg cgaccgccaa cgacgtgagc ccggccttgt gcgccttcca ccgcagtgcc
     6481 cccagcgtga cgaaaatcgt caccagagga agcaaggcga tgagtgcggt gagccacagg
     6541 ctgttgccca acgccgtggg attgggggtg tacgcagccg tcatgacgac cggcctcctt
     6601 cggggatcgg tgaacgggac tccgcgacaa ggtcgtcgag gagttcgagg acatgtcgat
     6661 agggctgccc ggtggcccgg gtcatgccca gctcgcacgt tcggttcgag gacacgaatg
     6721 cgtcgtatgc cgcgcggccc acctccgcgg cttcgcgccg ggtcgccgac gccgtcagtt
     6781 ccgggtgcag caggccccgg tcgccggcga aggcacagca gccggcgtcg tcggggacga
     6841 cgacccgctc ggcgcacgct tgggcgatgg ccaggagatg cgggacatcc ccggcatggc
     6901 ggtccgaaca ggtcgggtgg accatgaccg agccgagctg cctaggcacc gtcaggctcg
     6961 gcagcacccg ctcggccacg aaccgggtgc tgtcgacgat gtccagccgg gacacgcggg
     7021 aagcgagttc ggcttcaccc gcggccgcca catcttcggc caggctgtgc aacccatggg
     7081 tgcacgaact ggcttccgag acaacgggaa cctcgccatc gcggctcgcg ctgagtaggg
     7141 ccaccgcggt cgcgatcgcc atcgtccgca gcccgtcggt caagcccttg ctacgccata
     7201 ccgtgccgca gcacagcgtt tccaggccga cgggcagctg gacccgcacg ccggcggcgt
     7261 cgcacagccg caggaaagcg aacgccgccc cgcgcggacg agtggtggcg ccgggagacg
     7321 cggcgggcgc gaagagctca cccatgcagg aggcgaagaa gacgacatcg cccggctcgt
     7381 ggcgcgccgg tgtccgccgg ctgatgccgg gtcccgggag atcgtctcct acgcgtggga
     7441 cgaggtcaga tgaggcgatc gcccggccgg cctccgtcgc ggtcgtcagc agcccagccg
     7501 ggatgtgttc tgcgagagcg attccggagc gcaacgccgc cagcgtcgca tcccagtgcg
     7561 tggcgagccc cgcggcgatg cgttgcgccg cggccggctg ggcatcgtgc cggtggcgtt
     7621 tcatcacctt ccccgtgtcg atcccgacgg gacaggagag caggcacagg gagtccgcag
     7681 cgcaggtctc gaccgcggcg taggtgaagt cgcgggccaa ctcctcgcga cgcgccccct
     7741 cggcggccgc aagctcgcgc atgagtgcaa tccgctgacg aggtgtcgtc gtcaagtcct
     7801 ttgacggaca ggcgggttcg cagtagccac actcgacgca ccggtccacg gcggcgtcca
     7861 cgacctcagg gatcttgagg ttcttcagat gctggtcgcg gtcgtcggtg acgatgacac
     7921 cggggttgag gacatgggcc gggtcgaaga gccgcttgat ctcgcggatc acggcataca
     7981 actcagggcc gcactgccgc tcgacgtagg gggccatgat ccgcccggtc ccgtgctcgg
     8041 ctttcagcga accgtcggct gcgagaacga gatccaccag atcgtcgctg aaggcggcat
     8101 atcgctccag ctggaccggg tcgcgcagat cggggttgat catgaagtgg aggttggcgt
     8161 ccttggcgtg accgaagatc acggtgtcgc cgtagccgtg ccgggaacag agttcctgca
     8221 ggtccccgac aagcccggtc agtgccggca tcggcacgac gacgtcctcg agcaaagccg
     8281 tggagcccgt cggccgggcg ccggccaccg ccgtgtagag gcccttgcgc acctgccacc
     8341 gcacggcgcg ctcgcccggg tcgctggtga actcgaccgg gtagccgagg tcggtgagtc
     8401 cgccgagacc ggccacgacg cccccgagtc gggcgacacg ctcgcgcagt tcctccgggt
     8461 cgtcggcctg ggtctcgaca aggagggctg tatgccgcgc gaccgccaga tcgcgcaacg
     8521 ccggcaccgt gaccgggagg tcttgcgcga cacgcagcga actcgcgtcg agcagttcgg
     8581 cggtggcgac cccggccgcg acgagggcgg gcagcgcgtc ggtcgcatgg ctgatgtcgt
     8641 cgaagaccag gaacgccgtg ccggcatggg cgtgcagagg tacggtgtcg aaggtcgcct
     8701 cgacgatgaa gccgagcgtg ccctcggagc cgaccatgag gtgggccagg atctgtgccg
     8761 gggtgtcgaa gtccaggaag gcgttgagtc cgtagcccat cgtgttcttc atcgagaact
     8821 ggtgacggat ccggtcgacg gacgcggcat tgccgcgcac gcgatcgcgc aaccggagca
     8881 gacccgcgta caggtcgggc tcgagagccc ggagtgcctc gtcggcgtcg ggcgccccgg
     8941 tgtcgatgtg ggtccccgtc acgaggacga cctggatgct gctgagggtt cggtaggtat
     9001 ttgccgtggt cccgcaggtc atccctgagg agttgttggc cacgacgcca ccgatcgtgc
     9061 aggcgatctc gctggcgggg tcggggccga ggaggcggcc gtagggcgtg agccgggcat
     9121 tgaccgcccg caccgtcgcc cccggctggc accgcactcg cgcgccgccg tcgagcacct
     9181 cgacgtcgcg gaagccgcgc cgggtgtcga tcatgacgcc gtcggtgacc gcctgcccgg
     9241 acaaggaggt gccgcccgac cggaaggtga ccgatgcgcc ggcctgcgct gccgcggcaa
     9301 cggcagccgc gacatccgcg ccgtcgcggg cgaacacgac ggcctgcggc tggaggagat
     9361 agtgcgacgc gtcatgggcc atccgggagc ggtcgagctg gctgtccgcg acgcgtttct
     9421 ccccgacggc ggcctgcagc cgcgcgatga ggtcctgggt gggtccagtt tcactgtgaa
     9481 gcatcctcgc tcctcacctt ggtacgacca actcaaggta ggggcgtaaa tcggatcggt
     9541 caacggttgg tcatgcgata gtgaggaagg tgacgtatgc cgcacaaccc atcgacggga
     9601 cctccgacga ccgcgctgaa acctcgcgcg gtcgcccggg cggtcacacc tggcgaccga
     9661 tgccgcggtc gcgcacctac acctatgtga tcgaccaggt cgacgaacag atcgtgagcg
     9721 gggccctgcg ggtcggcgat cgcctgccac ccgagcgaga attcgccact ctgctcgggg
     9781 tcggtcgagg cgcagtgcgc gaggcgatcc gggtgcttga ggcctatggc gtggtccgga
     9841 ccgaccccgg tcccgagggc ggcacgacac tgacatcgat gcccagtgag gcgctcaccc
     9901 aggtcttgcg tctgcacatc gggttggcca acttccccat ggcggatgtg atcgaggcgc
     9961 gggcgatgct ggagcggtgg agcgcccgtc tcgccgctcg gcacggcagc gcggacgatc
    10021 tggccgcgat cgagcatgag ttgctccaga tggagaatgg ccgaagcgac agggaggcct
    10081 tcaacgagca cgacacggct tttcacgtgg cgatcgcccg cgcgtccaac aaccggctcg
    10141 tggcggatat gacgaccgcg ctccgcgact cgattcgctc gctggtgctc gaatccttcc
    10201 acggtcacgc cgactggccc gcgctcgcgg agcaactgtg cgccgatcac cgccgcgtgt
    10261 tcgaggcgat cgcgagcggg gatgaatccg cggccgccga tgccgtcgaa gagcacatcc
    10321 gctccgcata cgccacgctc ggctggggtc gcgcctgacg gtgctggcct agcccgggac
    10381 cggcccggtc atcgatcgac gggctcgcgg acctggcggc ggtcggtgcc accgacgagt
    10441 gacgacagca gccgctcctg gctgacgctg ccgcggcgga aggagcgacg caggaggagg
    10501 accaagccgg cggtggcggc atacggcacc agatagacga ggtccgcggc ggccggagtg
    10561 gccccctccg gatgagcgag actggtggac atcttcagag cgctgagcaa caagcccgcg
    10621 ccgatcagcg ctgctgcaac gacgaggaat ggatcccggt gacgcatccg gcagccccct
    10681 ttggcacctc tggcgaaccg ttctcgggac accgtagcgg gttggccgga ccgggggaac
    10741 acccttgcag acaccgatcg ccgggaaacc gtttacacgg gagaatcctt tgacacggtc
    10801 aaaacacctg gtcagaagtt ctgtgacgct gttggcagga tggctgtcgg cagaggcggt
    10861 ccatgccctc gccgcccggg cagtgctgcc cgcccggcgg cccgcaccgg gcgagggagg
    10921 tcttgccgtc gtcgttctcg gctatcacaa cgaggatccg gcggcgatca actggctcaa
    10981 ccggtggcgg gccgatgtag cggtgcgatc cattccgcgt gatgggcaag cgccgctgat
    11041 cgttgccagc ggcggctccc gccggccggg gctcagcgag gcggc
//
LOCUS       DAOPJG010000111        10996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6247_length_10996_cov_7.596289, whole genome shotgun sequence.
ACCESSION   DAOPJG010000111 DAOPJG010000000
VERSION     DAOPJG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6247_length_10996_cov_7.596289"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKU75_10665"
     CDS             complement(<1..398)
                     /locus_tag="PKU75_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81385.1"
                     /translation="MRVGAEWLRPAGLAWRAVVGIIVTTLVVLGTAVGKDEWWPFAPM
                     SQYAFLVKSDGVINSPFMEARTADGGLVRVPLSGTGIGLERSEMEGQLPRLIREPSLM
                     QAIAILHARRSPDEPRYTEIFLRNRQTALDA"
     gene            487..1104
                     /locus_tag="PKU75_10670"
     CDS             487..1104
                     /locus_tag="PKU75_10670"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPF81386.1"
                     /translation="MLNRYARAFFTKVFTPVAKLFLSLGISPDIVTIVGTLGVCFGAL
                     AFFPRGEFLIGTLVVTAFVFSDTIDGVMARMSGRSGKWGAYLDSTLDRMGDAAIFGGL
                     VLWYAGDGNNVVMAGLALACLILGSVVSYARARAEGLGMTANVGIAERAERLVAVLVS
                     TFLVGLGVPEVLLGVVLALLAFASLITVIQRMWAVRVQALAAVES"
     gene            1101..2039
                     /locus_tag="PKU75_10675"
     CDS             1101..2039
                     /locus_tag="PKU75_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HPF81387.1"
                     /translation="MIPRALDPRRLGARAGDEVTIAGYRLGWAGVRALPEGAAYRLFD
                     QVADALVRRGGKGVQRLRSNYARVRPELGPRELDALVREGMRAYMRYYCEAFRLPTLT
                     AEQVAARVRVRGDGPVRTRLAAGRPVVAFLGHMGNWDLAGAWCCAELGTVVTVAEKLE
                     PEAVFREFLAFRESLGMRIIPLGADTFPRLRQETRGPVVVPLLADRDLTGGGMSVDFC
                     GHQARMAVGPAALALATGSALHPVSIRHERRDGGWGILVTFHDEIPVPASGTTREKTA
                     AMTQGCADVLGGVIRRHTADWHMLQRVFADDLDGRR"
     gene            2039..3172
                     /locus_tag="PKU75_10680"
     CDS             2039..3172
                     /locus_tag="PKU75_10680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF81388.1"
                     /translation="MRVGLVCPYSFDVAGGVQFHVRDLAEWLGAHGHHASVIAPADED
                     AQLPDYFTSCGRAVPVRYNGSVARLTFGPVTAARVNRWLDAGNFDVVHIHEPMTPSAS
                     VLALRAFDGPIVATFHTSTLRSRAMSAAYPLLRPSLEKINGRIAVSEEARRTVIRHIG
                     GDAVVIPNGVDVGMFAKARVTPQWCGRPDAPTVGFLGRLDEPRKGLRVLAQAMDPVLH
                     AIPQARLLVAGPGDADEARAEMTPAVAAATEFLGALSNADKASFLHSIDLYVAPNTGG
                     ESFGIILVEAMAAGAPVLASDLPAFLRVLGDEAGASFANENTTALAGQIVRLLGDDAE
                     RVRLAAAGADRARDFDWSVVAEDILAVYDTVTPMGIRSRAWSR"
     gene            3201..3779
                     /locus_tag="PKU75_10685"
     CDS             3201..3779
                     /locus_tag="PKU75_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81389.1"
                     /translation="MVELITWIVLALAVVLGIAWYLSYSAARLDRLHAKVEGALSALD
                     ANLVRRAEASIELANSGVLDPASALLLASAASEALERETSHALPDDPLDGQHFGDRED
                     VETDLTEALAATLTEDVRAHIEASDGPAADELDRLVAAGRRVQFARNFHNQAVRDVQR
                     VRNKRVVRWFRLAGYAELPRPVEFADTVPGDR"
     gene            3879..4781
                     /gene="pdxS"
                     /locus_tag="PKU75_10690"
     CDS             3879..4781
                     /gene="pdxS"
                     /locus_tag="PKU75_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPF81390.1"
                     /translation="MSDPTPAPTTGTARVKRGMAEMLKGGVIMDVVTPDQAKIAEDAG
                     AVAVMALERVPADIRAQGGVSRMSDPDMIDGIIAAVSIPVMAKARIGHFVEAQVLQAL
                     GVDYIDESEVLTPADYANHIDKWAFTVPFVCGATNLGEALRRITEGAAMIRSKGEAGT
                     GDVSNATTHMRQIRQEIRRLQNLPPDELFVAAKELQAPYDLVREVAEAGKLPVVLFTA
                     GGIATPADAAMMMQLGAEGVFVGSGIFKSGNPAQRAEAIVQATTFYDDPDVIAKVSRG
                     LGEAMVGINVEEIPQPHRLAERGW"
     assembly_gap    4816..4915
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5141..5290)
                     /locus_tag="PKU75_10695"
     CDS             complement(5141..5290)
                     /locus_tag="PKU75_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81391.1"
                     /translation="MQLWAFQSNTAAIRFYERHGFRQVRWTDGDNDEGEPDVLMEWPG
                     APVRR"
     gene            5611..6333
                     /locus_tag="PKU75_10700"
     CDS             5611..6333
                     /locus_tag="PKU75_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF81392.1"
                     /translation="MPPTPRPAARGRARGVLSLEAIVDEAMRIIDREGIDEVSMRRIA
                     GEFDTGPASLYAHVANKDDLLAHCLRRVVSEVPMPTGETWQELVRSWAFGTHNTFVAH
                     GDIVRLTFGTIAEGPKFVDIMERILGALQAAHVPPRVATWGLDVLSLYVAADAYEGWI
                     MGRIFEDRSGRPTAEVGQEYFQQFAERLAALPAKQYPAITPNLTSLMSGGGDERFAFG
                     VDTFIAGMAAQVPAEGADRADG"
     gene            6326..6928
                     /gene="pdxT"
                     /locus_tag="PKU75_10705"
     CDS             6326..6928
                     /gene="pdxT"
                     /locus_tag="PKU75_10705"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPF81393.1"
                     /translation="MADPTIGVLAVQGDVREHLHALEASGATAIRVRRPAEVDVIDAL
                     VIPGGESTVIDKLVRAFGLHEPLRERLRAGMAAYGSCAGMIMLADRIEGGRLDQQTLG
                     GLDITVRRNAFGRQVDSFEEDLHIRVLGPQPLRAVFIRAPWVEAVGPEVQILARVETG
                     PATGRIVAVRQGPLLATSFHPEVTGDHRIHRYFVDLVRSP"
     gene            7037..7792
                     /locus_tag="PKU75_10710"
     CDS             7037..7792
                     /locus_tag="PKU75_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF81394.1"
                     /translation="MSGHSKWATTKHKKAAIDAKRGKLFAKLIKNIEVAAKTGGGDPD
                     GNPTLWDAIYKAKKSSVPNDNIERAVKRGSGAEAGGADWQNITYEGYAPGGVALLIEC
                     LTDNRNRAAAEVRTALTRNGGNLADPGSVAYIFNRKGVVIIPKGEKSEDDILEVVLDA
                     GAEEVNDNGDTWEIVSEASDVIAVRTALQDAGIDFDSAEASWVPNLQVPLDLEGARKM
                     IRVVDALEDSDDVQNVYANGDISDEVLAELEED"
     gene            7904..8455
                     /gene="ruvC"
                     /locus_tag="PKU75_10715"
     CDS             7904..8455
                     /gene="ruvC"
                     /locus_tag="PKU75_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPF81395.1"
                     /translation="MRVLGVDPGLTRCGLGVVDGKPGKACLVAVGVVRTPSDGDPGER
                     LVFLEKELNAWVDRFDPDVIAIERVFADVNVASVMTTAHATAIALLIAAKREIPVAFH
                     TPTEVKAAVTGDGRAGKAQVTSMVTRILKLKQRPKPADAADALALAICHTWRGGTEQR
                     YAAAARLAAVPSVPLARAAGVGR"
     gene            8452..9063
                     /gene="ruvA"
                     /locus_tag="PKU75_10720"
     CDS             8452..9063
                     /gene="ruvA"
                     /locus_tag="PKU75_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPF81396.1"
                     /translation="MIASLAGVVEYAGLDRLVVRVGGVGVLVHTPPATAGGAHTGAEI
                     TLATSLVVREDSLTLFGFDTPAEREMFETVQSVSGVGPRLALAMLSVMGPDELRLALS
                     TGDAKALTKIPGIGAKSADRLVLELRDKVGRLGSGGSPAGEPAASPTGAGGWREPVTE
                     ALVGLGWNAKQAADAVDKVAAGDQPDAGDIAAYLRLALRELRR"
     gene            9060..10184
                     /gene="ruvB"
                     /locus_tag="PKU75_10725"
     CDS             9060..10184
                     /gene="ruvB"
                     /locus_tag="PKU75_10725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPF81397.1"
                     /translation="MSPSGDGYASEIRQADQPVETSRVLDVAGSEDDRTVEATLRPRR
                     LADYAGQTRVRDQLDLVLQAAKARGTAPDHVLLSGPPGLGKTTLAMIVAAELERPIRV
                     TSGPAIQHAGDLAAVLSSLDEGEVLFLDEIHRMARAAEEMLYLAMEDFRVDVIVGKGA
                     GATAIPLELPPFTVVGATTRSGLLPAPLRDRFGFTGHLDFYAPDELAQILRRSAGLLG
                     VDADDDAVAEIAGRSRGTPRIANRLLRRVRDWAQVHGSGRVDLAATRAALELFDVDDQ
                     GLDRLDRAVLDALCRKYGGGPVGISTLAVAVGEEADTVETVAEPYLVRQGFILRTARG
                     RAASALAWSHLGLTAPPGAGPGVPGQATLPLDEPSAGRFD"
     gene            10302..10667
                     /gene="yajC"
                     /locus_tag="PKU75_10730"
     CDS             10302..10667
                     /gene="yajC"
                     /locus_tag="PKU75_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPF81398.1"
                     /translation="MLTGLATATQGQGLGIGNLLILLLPLILLAFLMMQQRKRVRQVS
                     DLQTSLQVGEEVVTTSGMYGRIVAFDDPAVVLEVSPGVRLRFDRRAIGARAPQASGPL
                     TPGSDAPEHPSPNDGPVSN"
     gene            10667..>10996
                     /locus_tag="PKU75_10735"
     CDS             10667..>10996
                     /locus_tag="PKU75_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPF81399.1"
                     /translation="MARQQSKPDTEKRTLLGFFALVLALAALLGGAHIWGTAALNPKL
                     GLDLKGGTQLVLSPRLADGQTVTSDQVAQARDIIAERVDSQGVSGAEVTTQGENNIVV
                     SIPENPSR"
BASE COUNT         1597 a         3916 c         3783 g         1600 t
ORIGIN      
        1 gcatcgagag ccgtctgccg gttgcggagg aagatctcgg tgtacctcgg ctcgtccggg
       61 ctgcggcggg catgcaggat ggcgatcgcc tgcatcagcg acggctcccg gatcaaccgc
      121 ggcagctgac cctccatctc cgagcgctcc agcccgatgc cggtgccgct caacggaacc
      181 cggaccaggc cgccatcggc cgtgcgtgcc tccatgaagg gggagttgat gacgccgtcg
      241 ctcttgacga ggaacgcata ctgcgacatc ggggcgaagg gccaccactc gtccttgccg
      301 acggcggtgc cgaggaccac cagcgtcgtc acgatgatcc ccaccaccgc gcgccacgcc
      361 agtcccgcgg gacgcagcca ctccgcaccc acccgcatac cgcgtatcgt cgcacggcgc
      421 gccgagggcc ccgcagcacc gtgatgcacg ggcgtggcgc ggcgggtcat ctaccctggt
      481 gccaccatgc tcaatcgata tgccagggcc ttcttcacga aggtcttcac cccggtggcc
      541 aaactgttcc tgtcactcgg catcagtccc gacatcgtca cgatcgtcgg gactctcggt
      601 gtgtgcttcg gggccctggc gttctttccg cgtggcgagt tcctcatcgg cacgctcgtc
      661 gtgaccgcct tcgtcttctc cgacaccatc gacggcgtga tggcccggat gtccggccgg
      721 tccggcaagt ggggcgccta cctcgactcg accctggacc gcatgggcga tgcggcgatc
      781 ttcggcggcc tggtgctctg gtatgccggc gacggcaaca acgtcgtcat ggccgggctc
      841 gcgctcgcct gcctgatcct cggcagcgtg gtctcctacg cccgcgcccg cgccgaaggg
      901 cttggtatga ccgcgaatgt cggaatcgcc gaacgcgccg aacgtcttgt ggccgtgttg
      961 gtttcgacgt tcctcgtcgg tctgggagtg cccgaggtgc tgctcggggt cgtgctcgcg
     1021 ttgctcgcgt tcgccagcct catcaccgtc atccagcgga tgtgggcggt gcgggtccag
     1081 gcgctggccg ccgtcgaatc gtgatcccgc gggcgctgga ccccaggcgg ctgggcgccc
     1141 gggccggcga cgaggtgacg atcgccggct accgcctcgg ctgggccggg gtgcgcgccc
     1201 tgcccgaggg cgcggcatac cgcttgttcg accaggtcgc cgacgcgctg gtgcgacgcg
     1261 gcggcaaggg tgtgcagcgt ctgcgctcca actacgcccg cgtgcggccc gagctgggtc
     1321 cgcgcgagct cgacgccctg gtgcgcgagg ggatgcgcgc ctacatgcgc tactactgcg
     1381 aggcgttccg gctgcccacg ctcacggcgg agcaggtcgc ggcgcgggtg cgcgtccgcg
     1441 gggacgggcc ggtgcggacc cggctggccg ccggccggcc cgtggtcgcc ttcctcggcc
     1501 acatgggcaa ctgggatctg gccggagcct ggtgttgcgc cgaactgggg accgtggtca
     1561 cggtggccga gaagctcgaa cccgaggccg tgttccggga gttcctcgcc ttcagggagt
     1621 cgctcgggat gcgcatcatc ccgctcggcg ccgacacctt cccgcggctg cggcaggaga
     1681 ccaggggtcc ggtcgtcgtg ccgctactgg ccgaccggga cctgaccggc ggagggatga
     1741 gcgtcgactt ctgcggacac caggccagga tggcggtcgg cccggcggcc ctggcgctcg
     1801 cgaccggctc cgcgctgcac ccggtctcga tccgccacga gcggcgcgac gggggctggg
     1861 gcatcctcgt caccttccac gacgagatcc cggtgccggc gagcggcacc acacgggaga
     1921 agacggcggc catgacccaa ggctgcgccg acgtgctcgg tggggtgatc aggcggcata
     1981 ccgccgactg gcacatgctc cagcgggtct tcgccgacga cctggacgga cgccgctgat
     2041 gcgcgtcggc ctggtctgcc cctacagctt cgatgtggcc ggcggcgtgc agttccacgt
     2101 gcgggatctg gccgagtggc tgggcgcgca cggccaccac gcgtcggtca tcgcgccggc
     2161 cgacgaggac gcccagctgc ccgactactt caccagctgt gggcgggcgg ttccggtgcg
     2221 ctacaacgga tccgtggccc ggctgacgtt cgggccggtg acggcggcgc gggtgaaccg
     2281 gtggctcgac gcgggcaact tcgacgtcgt ccatatccac gaacccatga cgcccagcgc
     2341 ctccgtgctg gcgctgcgcg ccttcgacgg gccgatcgtc gcgaccttcc acacgtccac
     2401 cctgcgctcg cgggcgatga gcgccgccta cccgctgctg cggccgtccc tggagaagat
     2461 caacggccgg atcgccgtct ccgaggaggc ccgccgcacc gtcatccggc acatcggcgg
     2521 cgacgccgtg gtcatcccga acggggtgga tgtcgggatg ttcgcgaagg cgagggtcac
     2581 cccgcagtgg tgcgggcgac cggacgcgcc gacggtcggg ttcttggggc gcctcgacga
     2641 gccccgcaag ggcctgaggg tgctggcgca ggcgatggat ccggtgctgc acgcgatccc
     2701 gcaggcgcgg ctgctcgtgg cagggcccgg cgacgccgac gaggcgcggg ccgagatgac
     2761 accggccgtg gccgcggcca cggagttcct cggggccctg tcgaatgccg acaaggcctc
     2821 gttcctgcac tccatcgacc tgtatgtcgc gcccaacacc ggcggggaga gcttcggcat
     2881 cattctggtc gaggcgatgg cggcgggggc gccagtgctg gccagtgacc tgccggcctt
     2941 cctgcgcgtg ctcggggacg aggcgggcgc ctccttcgcc aacgagaaca cgaccgcgct
     3001 cgcggggcag atcgtccgcc tgctcggcga cgacgccgag cgggtgcggc tcgcggcggc
     3061 gggtgccgac cgggcccggg acttcgactg gtcggtcgtc gccgaggaca tccttgcggt
     3121 ctatgacacg gtgacgccga tggggatcag gagccgggca tggagccgct gagcaatcgc
     3181 atcgcaaccc ggcaggaggc ctggtggaac tgatcacgtg gatcgtcctg gcgctggccg
     3241 tcgtcctcgg catcgcctgg tatctctcct acagcgccgc gcggctcgac cgcttgcacg
     3301 ccaaggtgga aggcgccctg tccgccttgg acgccaacct ggtgcgccgg gccgaggcca
     3361 gcatcgagct ggccaacagc ggtgtcctgg acccggcgtc cgccttgttg ctcgcgtcgg
     3421 ccgcctcgga ggccttggaa cgggaaacct cgcacgcgct gccggacgac ccgctcgacg
     3481 ggcagcactt cggcgaccgg gaggacgtcg agaccgacct gaccgaggcg ctcgcggcca
     3541 ccctcaccga ggatgtccgg gcgcacatcg aggccagtga cgggccggcc gcggacgaac
     3601 tcgaccggct cgtcgcggcc ggccgccggg tgcagttcgc ccgcaacttc cacaaccagg
     3661 cggtccggga cgtgcagcgc gtgcgcaaca aacgggtcgt gcgctggttc cggctggccg
     3721 gctacgccga actgccccgg ccggtcgagt tcgccgacac cgtgccgggc gatcgctagg
     3781 cgctttgtcc aacctccctc ccactgcgaa aaggcgacat agactcgcgg cgccgcccgc
     3841 gcggcatacc cgaccctgac gacaccgaag aggcacccat gtccgacccc acaccggcgc
     3901 ccaccaccgg cacggcccgc gtcaagcgcg gcatggccga gatgctcaag gggggcgtca
     3961 tcatggacgt cgtcaccccc gaccaggcca agatcgccga agacgccggc gcggtcgccg
     4021 tgatggcgct cgagcgggtg cctgccgaca tccgcgccca ggggggagtg tcgcggatga
     4081 gcgatccgga catgatcgac ggaatcatcg cggcggtctc gatcccggtg atggccaagg
     4141 cccggatcgg gcacttcgtc gaggcgcagg tgctgcaggc gctcggcgtc gactacatcg
     4201 acgagtccga ggtgctcact ccggccgact acgccaacca catcgacaag tgggccttca
     4261 ccgtcccctt cgtctgtggc gcaacgaatt tgggcgaggc gctgcggcgg atcaccgagg
     4321 gggcggcgat gatccggtcc aagggtgagg ccggcacggg cgatgtctcc aatgccacga
     4381 cccacatgcg gcagatccgt caggagatcc ggcggctgca gaacctgcct cccgatgaac
     4441 tgttcgtcgc cgccaaggag ttgcaggcgc cctatgacct ggtgcgcgag gttgccgagg
     4501 cgggcaagtt gccggtggtc ctcttcaccg ccggcggcat cgccactccg gcggacgccg
     4561 cgatgatgat gcagctgggc gccgagggcg tcttcgtcgg atccggcatc ttcaagtcgg
     4621 ggaacccggc gcagcgggcc gaggccatcg tgcaggcgac gaccttctat gacgaccccg
     4681 acgtcatcgc caaggtctcg cgtggcctgg gcgaggccat ggtcggcatc aacgtcgagg
     4741 agatcccgca gccgcaccgg ctggccgagc gcgggtggtg actggccgag cgcgggtggt
     4801 gacttcctga gggccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctggc
     4921 cgagcgcggg tggtgactgg ccgagcgcgg gtggtgactt cctgagggcc ccggatctgg
     4981 ccgtcctcgt cgtcggccgc tttccactcg ccgccgcggc ccttctcgaa ctgcctgaga
     5041 agacgcgaac tctcgccgaa agcccgaact gagtcacaaa tattcgaggg agttcgagcc
     5101 aaccgcctca gttcggggca acccagtcag tccgtccggg tcagcggcgt acgggcgccc
     5161 ccggccactc catgagcacg tccggctcgc cctcgtcgtt gtcgccgtcg gtccagcgca
     5221 cctgccggaa tccatgcctc tcgtagaagc ggatcgctgc ggtgttcgac tggaaggccc
     5281 acagctgcat accgccggga agcgctcctt ggccagggcc aagaggcggg cgccgaggcc
     5341 tcggccggtg tgctcccgaa tcagatagag gtgattgagg tggtcgggct cggcgagggc
     5401 gagaaagccg acgatctcgc cccgggttgt gtggtggtca tcgcaggact cctggcgaac
     5461 ggtgttcgtt gacgaacact gttctacaac gaacggcgtt cgtcgtccag ggcgatcatt
     5521 gcggccggtc atcgggggtg gacggcaggg cgcgccgccg cggcccgcct tagactcacc
     5581 gccttagact caccgcctta gactcaccgc atgccgccga cccctcgccc tgccgcccgg
     5641 ggccgcgcgc ggggggtcct cagcctcgag gcgatcgtcg acgaggccat gcggattatc
     5701 gaccgcgagg gcatcgacga agtgtccatg cggcgcatcg ccggcgagtt cgacaccggc
     5761 ccggccagcc tctacgccca cgtcgcgaac aaggacgacc tgctcgccca ctgcttgcgc
     5821 cgcgtcgtct cggaagtgcc gatgcccacg ggggagacat ggcaggagtt ggtgcgctcg
     5881 tgggccttcg gtacgcacaa tacctttgta gcacatggcg atatcgtccg cctgaccttc
     5941 ggcaccatcg ccgagggacc gaagttcgtc gacatcatgg agcgcattct cggcgcgctg
     6001 caggctgcgc acgtgccccc gcgcgtcgcg acgtggggcc tcgatgtcct ctcgttgtat
     6061 gtcgcggccg acgcctacga gggctggatc atgggccgga tcttcgagga cagatccggc
     6121 cggcccaccg cggaggtcgg gcaggagtac ttccagcagt tcgccgagcg gctggcggcg
     6181 ctcccggcga agcagtaccc cgcgatcacg cccaacctga cgagcctgat gagcggcggc
     6241 ggtgacgaac gcttcgcgtt cggtgtcgac accttcattg cgggtatggc cgcgcaggtc
     6301 cccgccgagg gcgccgaccg cgccgatggc tgacccgacc atcggtgtcc tggccgtcca
     6361 aggggatgtc cgggagcacc tgcacgccct ggaagccagc ggtgccacag ccatccgagt
     6421 gcgccgaccc gccgaggtcg acgtcatcga cgccctggtc atccccggcg gcgagtccac
     6481 ggtcatcgac aagctggtgc gcgccttcgg gctgcacgag ccgctgcgcg agcggctgcg
     6541 cgcggggatg gcggcatacg gctcgtgcgc gggcatgatc atgctcgccg accggatcga
     6601 gggcggccga ctcgaccagc agaccctggg tggtctggat atcacggtgc gccgcaacgc
     6661 attcggccgc caggtcgatt cgttcgagga ggacctccac atccgcgtac tcggccccca
     6721 gccgctgcgc gccgtcttca tccgggcgcc gtgggtcgag gctgtcggac ccgaggttca
     6781 gatcctcgcc cgcgtcgaga ccggcccggc gaccggccgg atcgtcgcgg tccggcaggg
     6841 cccactgctg gccacctcct tccaccccga ggtcaccggc gaccaccgca tccaccgcta
     6901 cttcgtcgac ctggtgcgct ctccctgacc gggcacagct gacggggccc ttgtggtcgg
     6961 cgcccgtggg cacagggcgg gcgcaggtag gatcgaccct cagtccgaac cgccatcgac
     7021 cactgaagga ccatgcatgt ccggccactc caaatgggcg accacgaagc acaagaaggc
     7081 cgccatcgac gccaagcgcg gcaagctgtt cgccaagctg atcaagaaca tcgaggtcgc
     7141 ggcgaagacc ggcggcgggg acccggacgg caacccgacg ctgtgggacg ccatttacaa
     7201 ggccaagaag tcctcggtgc ccaatgacaa catcgagcgc gccgtcaagc gcggctccgg
     7261 cgccgaggcc ggcggcgccg actggcagaa catcacctac gagggctacg cccccggcgg
     7321 tgtcgccctg ctcatcgagt gcctcacgga caaccgcaac cgcgccgccg ccgaggtgcg
     7381 caccgccttg acccgaaacg gcggcaacct ggccgaccct ggctccgtcg cctacatctt
     7441 caaccgcaag ggcgtcgtca tcatccccaa gggcgagaag tcggaggacg acattctcga
     7501 ggtcgtcctc gacgccgggg cggaggaggt caacgacaac ggcgacacct gggagatcgt
     7561 ctcggaggcc tccgacgtca tcgccgtgcg caccgccctc caggacgccg ggatcgactt
     7621 cgactccgcc gaggcctcct gggtccccaa cctccaggtc ccgctcgacc tcgaaggcgc
     7681 ccgcaagatg atccgcgtcg tcgacgccct cgaggactcc gacgacgtcc agaacgtcta
     7741 cgccaatggc gacatctccg acgaggtgct cgcggagctc gaagaggact gacctcgtca
     7801 cgacacggaa gggcccggcg cgtgttgctg ccgggccctt cgcgcatccg tgggtagcct
     7861 gatcttccga acagatgttc gccagacggg aagcaggtgc agcgtgcgtg tcctcggagt
     7921 cgaccccggc ctgacccgat gcggtctggg cgtggtcgac ggcaagcccg gcaaggcctg
     7981 cctcgtggcc gtcggcgtgg tgcgcacccc gagcgacggt gaccccggtg agcggctggt
     8041 cttcctcgag aaggaactca acgcctgggt ggaccgcttc gaccccgacg tgatcgccat
     8101 cgaacgcgtc ttcgccgacg tcaatgtcgc ctccgtcatg acgacggcgc acgccacggc
     8161 catcgccctg ctcatcgccg ccaaacgcga gatccccgtc gccttccaca cgccgaccga
     8221 ggtcaaggcg gcggtcaccg gcgacggtcg ggccggcaag gcgcaggtca cctcgatggt
     8281 cacccggatc ctgaagctca agcagcgccc caaacccgcc gacgctgccg acgcgctcgc
     8341 gctggccatc tgccacacgt ggcggggggg caccgagcag cggtatgccg cggccgcccg
     8401 cctcgccgca gtgccctccg tgccgctcgc ccgcgctgcc ggagtgggcc gatgatcgcc
     8461 agcctcgccg gcgtcgtgga gtatgccggc ctggaccgtc tcgtcgtgcg tgtcgggggc
     8521 gtcggggtgc tggtgcacac gccgccggcc acggcgggtg gggcgcacac gggggccgag
     8581 atcaccctgg ccaccagcct cgtcgtgcgc gaggacagcc tgaccctgtt cggcttcgac
     8641 accccggccg aacgggagat gttcgagacc gtgcaaagcg tctccggcgt cgggccgcgg
     8701 ctggcactgg ccatgctgtc ggtgatgggg cccgacgagt tgcgcctcgc cttgtccacc
     8761 ggcgacgcga aggcgctgac caagatcccg ggcatcggtg ccaagtccgc cgaccgcctc
     8821 gttctcgagt tgcgcgacaa ggtcggccgg ctcggcagcg gcggctcccc ggcgggggag
     8881 ccggccgcct cgccgaccgg tgccggcggc tggcgcgaac cggtcaccga ggccctcgtc
     8941 gggctgggct ggaacgcgaa gcaggccgcc gatgccgtcg acaaggtcgc cgccggtgac
     9001 cagcccgacg ccggcgacat cgcggcatac ctgcggctgg ccctgcggga gctgcggcga
     9061 tgagcccatc cggcgacggc tacgcgagcg agatccggca ggccgaccaa cccgtcgaga
     9121 ccagccgggt cctcgacgtg gctggctcgg aggacgatcg cacggtcgag gcgacgttgc
     9181 ggccgcgccg cctggccgac tacgccggcc agacccgggt gcgcgaccag ctcgacctcg
     9241 tgctgcaggc cgccaaggcg cgcggcaccg cccccgacca tgtcctcttg tccggcccgc
     9301 ccggactggg caagaccacg ctggcgatga tcgtcgccgc cgaactcgag cgaccgatcc
     9361 gggtcacgag cggtccggcg atccagcacg ccggcgacct ggccgccgtc ctgtcctccc
     9421 tcgacgaggg agaggtgctc ttcctcgacg agatccaccg catggcccgc gcggcggagg
     9481 agatgctcta tctcgcgatg gaggacttcc gcgtcgacgt catcgtcggc aagggggccg
     9541 gagccactgc cataccgctg gagctgccgc ccttcaccgt cgtcggcgcc accacccgct
     9601 ccggcctgct gcccgcgccc ctgcgcgacc gcttcggttt caccggccac ctcgacttct
     9661 atgcgcccga cgagctggcg cagatcctgc ggcgctcggc cgggctgctc ggcgtggatg
     9721 ccgacgacga cgccgtcgcc gagatcgccg gccgctcccg cggcacgccg cggatcgcca
     9781 accggctgtt gcgccgcgtg cgcgactggg cgcaggtgca cggcagcggc cgggtcgacc
     9841 tggcggccac gcgtgcggcg ctcgagctct tcgacgtcga cgaccagggc ctcgaccgct
     9901 tggaccgggc agtgctggac gcgctgtgcc gcaagtatgg cggcggtccc gtcggcatct
     9961 ccacgctcgc cgtcgcggtg ggggaggagg ccgacacggt cgagacggtg gcggagccct
    10021 atctggtgcg gcagggcttc atcctgcgca ccgcccgggg gcgggccgcc agcgctctcg
    10081 cctggagcca cctcggcctc accgcgcccc ccggcgccgg ccctggcgtc ccaggtcagg
    10141 ccacgctgcc gctcgacgag ccctccgcgg gccgcttcga ctagccggcg gaacgcgccg
    10201 agcgggtcgc gcgtttgggc cgacagcgag gacgtctcgt agactccctc ggtggccccg
    10261 atgggccaac gatcttgcac gcccacgaag gacaactctc gatgcttacg ggcctggcga
    10321 ccgccactca gggccaggga ctcggaatcg gcaatctgct gatcctgttg ctgcccctga
    10381 tcctccttgc tttcctcatg atgcagcagc gcaagcgagt gcgtcaggtc agcgacctcc
    10441 aaacctcgct gcaggtcggt gaggaagtgg tcaccacctc cgggatgtat ggccggatcg
    10501 tcgcgttcga cgaccccgcg gtggttctcg aggtgtcgcc cggtgtccgg ttgcggttcg
    10561 atcgccgcgc catcggggcg cgcgcgccac aggcgagcgg cccgctcacc ccgggttcgg
    10621 acgcccccga acacccctcc cccaacgacg gaccggtgag caactgatgg cgcgccaaca
    10681 gagcaaaccc gacaccgaga agcgcactct cctcggtttc ttcgccctgg tcctcgccct
    10741 tgccgcgctg ctcggcgggg cgcacatctg gggcaccgcg gcgctcaacc ccaagctcgg
    10801 cctcgacctc aagggcggca cccaactggt gctgagcccg cggctcgccg acgggcagac
    10861 cgtcaccagc gaccaggtcg cccaggcccg cgacatcatt gccgagcgcg tcgactctca
    10921 gggagtgagc ggcgccgagg tgaccacgca gggcgagaac aatatcgtcg tctcgatccc
    10981 ggagaacccg agccgg
//
LOCUS       DAOPJG010000112        10951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6273_length_10951_cov_7.336546, whole genome shotgun sequence.
ACCESSION   DAOPJG010000112 DAOPJG010000000
VERSION     DAOPJG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6273_length_10951_cov_7.336546"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKU75_10740"
     CDS             complement(<1..534)
                     /locus_tag="PKU75_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPF81400.1"
                     /translation="MQDPTQLYRFEPDLDARTLGPGVLLLGLGGFIDAGNTSAILVKH
                     LLDASSARVVASFDVDQLLDYRGRRPTMVFEENHWVSYDDPALLLYQLTDRDGQTYYL
                     LTGPEPDYQWERVIEAVRGLMQRLGITLLVNAHGIPMAVPHTRPIGMTAHATDARLVG
                     EHSPVFGRVQVPAGLAAL"
     gene            complement(613..4410)
                     /gene="hrpA"
                     /locus_tag="PKU75_10745"
     CDS             complement(613..4410)
                     /gene="hrpA"
                     /locus_tag="PKU75_10745"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HPF81401.1"
                     /translation="MDQGVEAPRRLDLPELNYPSQLPVVERIDDIATAIRDHQAVVIA
                     GETGSGKTTQIPKIALQIGRGTHGQIGLTQPRRIAARSVAERISDELGVELGGLIGYQ
                     VRFTDTSSADTAVKVMTDGILLNELQRDRDLRRYDTIIIDEAHERSLNIDFILGYLKR
                     LLPRRPDLKVIITSATIDPQRFADHFAAPDRPVPVIEVSGRMYPVEVRYRPLVDERGA
                     GTDQITGVCEAVEELWTEAGGHDGGDHDILAFFSGEREIRDAADALTAMKLPHTEIVP
                     LFARLSAAEQHRVFAPHRGRRIVLATNIAETSLTVPGIRYVVDTGTARISRYSQRTKV
                     QRLPIEPISQASARQRAGRCGRLADGICIRLYSEEDFESRPAFTEPEILRTSLAAVIL
                     QMVSLRLGDVARFPFVDPPDSRNIADGIRLLKELHALQETRRAAGERLTSIGRSLARL
                     PVDPRLGRMLLEADKLGCTREVLVIVAALSIQDPRERPSEQQTQADQSHARFRVPGSD
                     FGAYVGLWTYLQEQQKALSRSAFRRMCQREFLHYLRVREWQDLHQQLKSACKSLGIDP
                     NRATAARDAQPAWDTVHRALLAGLLSHIGLRDESTRDYLGARGARFAISPGSALFRKQ
                     PAYVMAAELVETSRLWARVNAGIDPLWAEEAGAHLVKRTYAEPRWSRRQGSVVASERV
                     TLYGVPLAVDRTVQFGRIDPELSRDLFIRHALVADDWDSHHPFVKANRALRQRIENME
                     DKARRRDIVAADSQLHAFFDARIPPGVVSAQHFDRWWKGEQRHHPDLLTLTEADLTNA
                     AADGVASQDYPGTWPAGDLELRLSYQFDPGSPDDGVSVRIPLTVLNRVEPEGFDWLVP
                     GMREDLVAALIRSLPKGTRTNFVPAPNHAAYVAREIRPGEGRITDEVARILREHTGIR
                     VDPQEWDWAKVPPHLRMTFVVTGDRGEVIGKDKDLNALREKLSRQVTRRVAKAGAAIE
                     QTGLTTFPDRPIPATYTSRAGGHEVLGYPALVDEGTSVALRVLPSQAAAAAAHRLGLR
                     RLLLLGTSPPWKRVLAGLSTADKLALQLAPHGSVPALLEDALAAAVDGLVADQPHAQI
                     RTTDDFGAALAAVRTHAAARVLAMVRTIGPVLQQAHVLRGRLEQLTAPGLSATVADAR
                     AQLDSLVGPGFVARVGVTHLNQLPRYLKALDQRLTKATADPARDRRLLDQLDPVEAAY
                     ADLLAALPAIERDSDPVRDIGWMIEELRVGLFAQGLGTSYPVSVKRVQKAIAEHAAAA
                     RAG"
     gene            complement(4469..5785)
                     /locus_tag="PKU75_10750"
     CDS             complement(4469..5785)
                     /locus_tag="PKU75_10750"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HPF81402.1"
                     /translation="MSDADWKFETRQVHAGQQADPTTGARALPIYQTTSYVFKDTDHA
                     ANLFALKEFGNIYTRIMNPTQDAVEQRLASLEDGVGALLVSSGQSATTIGLLNVAETG
                     DHIVASAALYGGTFNLLKYTFAKMGIEVTFVEDQANLDEWRAAIKDNTKVLFGETIAN
                     PKCDVLDIEGIAAIAHDAGVPLVVDNTIATPYLIRPLEWGADVVLHSATKYLGGHGTS
                     IAGVIVDGGTFDYAKDPAKWPNYNEPDESYHGLRYGPDLGVGSAFGANLSYILKARVQ
                     LLRDMGNAVSPFNAFLISQGIETLSLRIDRHLENTRKVVDFLAGHDQVTEVRWASRDG
                     DPGKERADKYAPKGAGAVIGFEIEGGIEAGKKFVEGLTLHSHVANIGDVRSLAIHPAS
                     TTHSQGGDEDRLAAGVTPGLIRLAVGIEHSDDILADLEQGFAAART"
     gene            5896..6321
                     /locus_tag="PKU75_10755"
     CDS             5896..6321
                     /locus_tag="PKU75_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HPF81403.1"
                     /translation="MSDYRPSPSGWVRDQVEAIEAAGTTDVASIMDRQVVLLTMIGRK
                     TGDTLKVPLMRVEHDGRYAAVASKGGAPDHPQWYYNLKANPDITVQDGTTVHDVRARV
                     LEGEERAQWWDRCTAAFPPYVEYQEKTDRIIPVFVLEPR"
     gene            complement(6399..6818)
                     /locus_tag="PKU75_10760"
     CDS             complement(6399..6818)
                     /locus_tag="PKU75_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81404.1"
                     /translation="MPSAERTPSATAQETGHGLSTGPGRLIVALYAVLALAATGRSIL
                     QITQYFDRAEVAYVLSALAALIYIVATYALATGRRALALTTITIELVGVLVVGAASYA
                     MSDSFPDKTVWSHFGSGYGYVPLVLPVIGLWWLRRAR"
     gene            complement(6805..7173)
                     /locus_tag="PKU75_10765"
     CDS             complement(6805..7173)
                     /locus_tag="PKU75_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81405.1"
                     /translation="MIIPLSVGVIALSVVWMLLAAFYAVRDRLINDLLLAVAVLTELG
                     LIVLAVLSAKGFRTIDPSSEGATFLAYALSLPAVPPFAALLAIREKSRWAMGVMVVAA
                     FTVAVMTARIAQIWNLYAVR"
     gene            complement(7188..7571)
                     /locus_tag="PKU75_10770"
     CDS             complement(7188..7571)
                     /locus_tag="PKU75_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81406.1"
                     /translation="MSAMDTLPMLRLPASWPLVVNATLAMTALALIDLVAAYAAKEWV
                     AHRAPAMAGLGACAMVLLFWVYASALQYAELAVVTLGWIVLLQLGVMVLDTLKYGVDY
                     PARTWIIVGGIMAAQGYLVITSSSR"
     gene            7734..9044
                     /locus_tag="PKU75_10775"
     CDS             7734..9044
                     /locus_tag="PKU75_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF81407.1"
                     /translation="MTDPRYQPAPEVELTHPEWSKNATIYQINTRQFSEEGTLRAAAE
                     QLPRIKDLGVDIVWLMPVNPIGEVNRKGSLGSPYAVQDYFAVNPDLGTLQDLKDFVAQ
                     AHSLGLYVILDWVANHTAWDNVWVSEHPEWYARDWKDEFTPTPWWDWDDIIDLDYSVP
                     ELRAEMTRALLYWVSEADIDGYRCDVAGFVPNDFWENARREMEAIKPVFLLAEWEARD
                     LHVRAFDMSYAWSWNDTMHHIAQGKADVEHLYIYYAWNAKAYPRDAMRMMFVSNHDKN
                     SWDGTEHEMFGDALEAAIVLSVIGEGMPLIYNGQEAGNDKRLKFFEKDPIVWREHPMA
                     DFYRDLFGLKKSLSCLWNGAWGARMVNIRNTAYKQVLSFVRQDDTSRVFCILNLSPTE
                     QTVTFGPGPHHGHWRDHGTGETFDVAADTTMTLGPWAYRILVGA"
     gene            9097..9576
                     /locus_tag="PKU75_10780"
     CDS             9097..9576
                     /locus_tag="PKU75_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81408.1"
                     /translation="MDARSEYDAAITEREIGVLLAGHRRALGALRASDRGVVLDVIRG
                     ELLAGTRTEPTDLDALARFVVFAEVRRPGKLLDALPEAIADRLRLGVVLALPVTADPP
                     RRRPILAHLDDTRPPEVGSWDLEGDPIYQHTIFCNMMQHVTDPGDVWRRRQRPPGIR"
     gene            9806..10363
                     /locus_tag="PKU75_10785"
     CDS             9806..10363
                     /locus_tag="PKU75_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HPF81409.1"
                     /translation="MSTLRPGRAEEAGLSRSGLYRAAREGRLERIARGIYLPADAPAA
                     DWDWIEVATRRPDATICLESALAHHDLTDVIPNVLDVAIPRGSRTPASTGAIAWHQFD
                     RATVEIGREEIPIPGTDQSIGIYSPERSIADAFRLRAEVGYELPRESLKEWLRRGGKP
                     ARLIEIASRLPRAKSPVLQALEALA"
BASE COUNT         1791 a         3520 c         3892 g         1748 t
ORIGIN      
        1 cagggcggcc agccctgccg gcacctgcac acgtccgaag acggggctgt gctccccgac
       61 gaggcgggcg tcggtcgcgt gggcggtcat gccgatcggg cgggtgtgcg gcaccgccat
      121 cgggatgccg tgggcgttga cgagcaaggt gatgccgagt cgctgcatca gcccgcgaac
      181 ggcctcgatg acccgctccc actgatagtc cggctcggga ccggtgagca ggtagtaggt
      241 ctggccgtcg cggtcggtga gctgatagag gagcagagcg gggtcgtcgt aggagaccca
      301 gtggttctcc tcgaagacca tcgtgggtcg ccgcccgcga tagtcgagaa gctggtcgac
      361 gtcgaagctg gcgacgacgc gcgcgctgga ggcgtcgagc aggtgcttga cgaggatggc
      421 cgaggtgttg ccggcatcga tgaacccgcc gagaccgagc agcagcactc ccgggccgag
      481 ggtgcgcgcg tcgaggtcgg gttcgaagcg atagagctgg gtggggtcct gcacggggat
      541 ccttcggttc gtcggacgca tggtcatcaa cgtggttcaa cgacgactgc gggcagttga
      601 ttccaccgcg ggtcagcccg cgcgggccgc agcggcgtgt tcggcgattg ccttctgcac
      661 ccgtttgaca ctcaccgggt atgacgtgcc gagaccctgc gcgaacaggc cgacgcgcag
      721 ttcctcgatc atccagccga tgtcgcgcac ggggtcgctg tcgcgttcga tcgcggggag
      781 agccgcgagc aggtccgcgt atgccgcctc caccgggtcg agctggtcga ggagccggcg
      841 atcccgggcc gggtcggcgg tggccttggt gaggcgttgg tcgagcgcct tgaggtagcg
      901 cggcagctgg ttcaggtggg tgaccccgac gcgcgcgacg aagccggggc cgacgagact
      961 gtcgagttgc gcccgggcgt cggcgaccgt cgcggacaga cccggggcag tgagttgctc
     1021 cagccggccg cgcaggacat gggcctgctg cagcaccggt ccgatcgtgc gcaccatcgc
     1081 cagcacgcgg gctgcggcat gcgtgcggac ggccgcgagg gcggcgccga agtcgtccgt
     1141 ggtgcggatc tgcgcgtggg gctggtcggc gacgagaccg tcgacggccg ccgcgagcgc
     1201 atcttcgagc agggcgggca cggagccgtg cggggcgagt tggagagcca gcttgtcggc
     1261 cgtgctcagg ccggcgagga cccgtttcca cggcgggctg gtgccgagca gcaggagccg
     1321 gcgcagaccg agccggtgtg ccgcggcggc cgcggcctgc gacggcagca cgcgcagtgc
     1381 cacactcgtg ccctcgtcga cgagcgctgg gtatccgagc acctcgtggc caccggcccg
     1441 gctggtgtag gtcgcgggga tgggcctgtc ggggaaggtg gtcaggccgg tctgctcgat
     1501 ggcggcaccg gccttggcga cccgacgggt cacctgacgg gaaagcttct cccgcaacgc
     1561 gttcaggtcc ttgtccttgc cgatgacctc gccgcggtcg cccgtgacga cgaatgtcat
     1621 ccgcagatgc ggcgggacct tggcccaatc ccactcctgc gggtcgacac gaataccggt
     1681 gtgctcacgc aggattcgcg cgacctcgtc ggtgatgcgt ccctcccccg ggcggatctc
     1741 ccgggcgacg tatgccgcgt ggttgggtgc gggcacgaag ttggtgcgcg tgcccttggg
     1801 cagcgagcgg atcagggcgg cgacgaggtc ttcgcgcatg ccgggcacga gccagtcgaa
     1861 gccctcgggc tcgacccggt tgaggacggt gagcgggatc cggacgctga ccccgtcgtc
     1921 cggggagcca gggtcgaact ggtagctcag gcgcaactcc aggtcgcccg ccggccaggt
     1981 gcccggatag tcctgggagg caacgccatc ggcggcggca ttggtgaggt cggcctcggt
     2041 gagggtgagc aggtcggggt gatgccgttg ctcgcccttc caccagcggt cgaagtgctg
     2101 ggcggagacg acaccgggtg ggatgcgcgc gtcgaagaag gcatggagtt gactgtccgc
     2161 ggcgacgatg tcgcgccgtc gtgccttgtc ctccatattc tcgatgcgtt gccgcagagc
     2221 acgattcgcc ttgacgaacg ggtggtggct gtcccagtcg tccgcgacga gagcgtggcg
     2281 gatgaacagg tcgcgggaga gctccgggtc gatccggccg aactggacgg tgcgatcgac
     2341 ggccagcggc actccataca gcgtcacgcg ttccgaggcg accacgctgc cttgcctgcg
     2401 cgaccagcgc ggctcggcat acgtccgctt gaccaggtgg gccccggcct cttccgccca
     2461 cagggggtcg atgccggcgt tgacgcgggc ccaaagacgt gacgtctcga cgagttccgc
     2521 ggccatgaca taggccggct gcttgcgaaa gagcgccgag ccgggtgaga tcgcgaagcg
     2581 agcgccgcgg gcaccgaggt agtcgcgtgt ggactcgtcg cgcagaccga tgtgcgagag
     2641 caggcccgcg agcagtgcgc ggtgaaccgt gtcccaggcc ggctgggcgt cgcgcgcggc
     2701 ggtcgcgcga ttggggtcga tgccgaggct cttgcacgcg gacttcagct gctgatgcag
     2761 gtcctgccat tcccgcacgc gcaggtagtg gaggaactcg cgctggcaca tccgccggaa
     2821 agcgctgcgg gagagcgctt tctgctgctc ctgcaggtag gtccacagac cgacgtaggc
     2881 accgaagtcg ctgcccggga cgcggaaccg ggcgtgggac tggtcggcct gggtctgctg
     2941 ctcgctcggt cgctcacggg ggtcctggat ggacagcgcc gcgacgatga ccaacacctc
     3001 tcgggtgcag ccgagtttgt cggcctccag gagcatgcgc cccagccgcg ggtcgaccgg
     3061 caaccgggcc aggctgcgtc cgatcgacgt cagccgctcc cccgccgcac gacgggtctc
     3121 ttggagggcg tgcagttcct tgagcagccg gatgccgtcg gcgatattgc gggagtcggg
     3181 cggatcgacg aaggggaagc gcgcgacatc gccgagccgg aggctgacca tctggaggat
     3241 gaccgcggcg agagaggtgc gcaggatctc cggctcggtg aaggccggcc gggactcgaa
     3301 gtcctcctcg ctgtagagac ggatgcagat accgtccgcg agccggccgc accggccggc
     3361 ccgctggcgg gcactggcct ggctgatcgg ctcgatcggc aggcgttgca ctttggtgcg
     3421 ctgggagtag cgggagatcc gcgcggtgcc ggtgtccacg acataccgga tcccgggcac
     3481 ggtgagggag gtctcggcga tattggtcgc caggacgatc cgccggccgc ggtgcggggc
     3541 gaacacccga tgctgctcgg cggccgaaag ccgggcaaag agcgggacga tctcggtgtg
     3601 cggcaacttc atcgccgtca atgcgtctgc ggcgtcacgg atttcgcgct cacccgagaa
     3661 gaaggcgagg atgtcatggt caccgccgtc gtggccaccg gcctcggtcc acagctcctc
     3721 gacggcctcg cagacgccgg tgatctggtc cgtgccggcg ccccgctcgt cgaccagtgg
     3781 gcgatagcgc acctccaccg gatacatccg gcccgagacc tcgatgaccg ggaccggccg
     3841 gtccggggcg gcgaagtgat cggcgaagcg ctgcgggtcg atggtcgccg aggtgatgat
     3901 caccttcagg tccggccggc gcggcagcaa ccgtttgaga taacccagga tgaagtcgat
     3961 attgaggctg cgctcgtgcg cctcgtcgat gatgatcgtg tcgtagcggc gcaggtcgcg
     4021 gtcgcgctgc aactcgttga gcaggatgcc gtccgtcatc accttcacgg ccgtgtcggc
     4081 gctcgaggtg tcggtgaagc gcacctggta gccgatcagg ccgccgagct ccacgccgag
     4141 ctcgtcactg atccgttcgg cgaccgagcg ggcggcgatc cgtcgcggct gggtcagccc
     4201 gatctgtccg tgcgtgccgc gcccgatctg cagggcgatc ttggggatct gcgtggtctt
     4261 gcccgacccg gtctcccccg cgatgacgac ggcctggtgg tcgcggatcg ccgtggcgat
     4321 gtcgtcgatc cgctcgacga ccggcagttg cgacgggtag ttcagctccg ggaggtcgag
     4381 gcggcgcggc gcctcgactc cctgatccac cccagcatcc tacggacgat cgacacctcg
     4441 atgcccagat cgaccgctcg acgggtggtc aggtgcgggc ggcggcgaag ccctgttcga
     4501 ggtcggcgag gatgtcatcg ctgtgctcga tcccgacggc gagacggatc aggccggggg
     4561 tgacgccggc tgcgagccgg tcctcgtcgc cgccttggct gtgggtggtc gaggcggggt
     4621 ggatcgccag ggagcggaca tcgccgatgt tggcgacgtg ggagtgcagg gtcaggccct
     4681 cgacgaactt cttgcccgcc tcgatgccgc cctcgatctc gaagccgatg accgcgcccg
     4741 cacccttggg ggcgtacttg tcggcgcgct ccttgcccgg gtctccgtcg cgggaggccc
     4801 agcggacctc ggtgacctgg tcgtgaccgg cgaggaagtc gacgaccttg cgagtgttct
     4861 ccaggtgccg gtcgatgcgc aggctgaggg tctcgatgcc ctgggagatg aggaaggcgt
     4921 tgaagggcga gacggcgttg cccatgtcgc gcagcagttg cacgcgggcc ttgaggatgt
     4981 aggacaggtt ggcgccgaac gcagagccga caccgaggtc gggcccgtag cgcaggccgt
     5041 gatagctctc gtcgggctcg ttgtagttcg gccacttcgc cgggtccttg gcgtagtcga
     5101 aggtgccgcc gtcgacgatc acaccggcga tcgaggtgcc gtgtccgcca aggtatttcg
     5161 tcgccgagtg caggacgacg tcggcacccc actcgagcgg gcggatgagg tatggcgtgg
     5221 cgatcgtgtt gtcgacgacc aacggcactc cggcgtcatg ggcgatggcg gcaatgcctt
     5281 cgatatccag cacatcgcac ttggggttgg cgatcgtctc gccgaagaga accttggtgt
     5341 tgtccttgat cgccgcgcgc cactcgtcga ggttggcctg gtcctcgacg aaggtgacct
     5401 cgatgcccat cttggcgaag gtgtatttca ggagattgaa ggtgccgccg tagagcgccg
     5461 cgctggcgac gatgtggtcg ccggtctcgg cgacattgag cagtccgatg gtcgtcgcgg
     5521 actggccgga ggagaccagc agggcgccga caccgtcttc gagcgaggcg agccgctgct
     5581 cgacggcgtc ctgggtcggg ttcatgatcc gggtgtagat gttcccgaat tccttgagcg
     5641 caaacaggtt cgccgcgtgg tcggtgtcct tgaagacgta tgacgtggtc tggtagatcg
     5701 gcagcgcccg cgcccccgtg gtcgggtcgg cctgctgccc cgcgtggacc tgccgggtct
     5761 cgaacttcca gtcggcgtcg ctcatcaccg tctccttgct gccgtatgcc gttgcctcac
     5821 cttctcgcag attctcagca tccgggatcc ccgtctcccg atccggacga cgccggactc
     5881 tgcgatgatc acctcatgag cgattaccgc cccagtccct ccggatgggt tcgcgaccag
     5941 gtcgaggcca tcgaggccgc cggcaccacc gacgtcgcca gcatcatgga ccggcaggtg
     6001 gtgctgctga ccatgatcgg acgcaagacg ggcgacaccc tcaaggtgcc gctgatgcgc
     6061 gtcgagcacg acggccggta tgccgccgtc gcgtcgaagg gcggcgcacc ggaccacccg
     6121 cagtggtatt acaacctcaa ggccaacccg gacatcaccg tccaggacgg caccaccgtg
     6181 cacgacgtcc gggcacgcgt cctcgagggc gaggagcgcg cgcagtggtg ggaccgctgc
     6241 accgcggcct tcccgccgta tgtcgagtac caggagaaga cggaccggat cattccggtc
     6301 ttcgtgctcg aaccccgata ggcccaggtg accatgcccc gccgcgatgc tccttcgggg
     6361 acggcaggtc gcgcccggtc gggcgctgca tccgccagtc agcgagcgcg gcgcagccac
     6421 cagaggccga tgaccggcag gaccagcggc acatagccgt aaccggatcc gaagtggctc
     6481 cacacggtct tgtccgggaa gctgtcactc atggcgtagg acgccgcgcc cacgacgagc
     6541 actccgacca actcgatggt gatcgtcgtc agggccagtg cgcggcggcc ggtcgccagc
     6601 gcgtacgttg cgacgatgta gatcagcgct gccagggcgg acagcacgta ggcgacctcg
     6661 gcgcggtcga agtactgggt gatttgcagg atcgagcggc cggtggcggc cagggcgagc
     6721 acggcataga gggcgacgat cagccggccg gggccggtgg acaggccgtg acccgtctcc
     6781 tgcgcggtgg cggagggtgt gcgttcagcg gacggcatag aggttccaga tctgcgcgat
     6841 gcgagcggtc atgacagcga cggtgaaagc ggccacgacc atcacgccca tcgcccagcg
     6901 cgacttctcg cggatcgcga gcagcgcggc gaagggcggg acggccggca ggctcaaggc
     6961 ataggcgagg aaggtcgccc cctcggagct ggggtcgatg gtgcgaaaac ccttggcgga
     7021 caggacggcc agcacgatca gtccgagttc ggtcaggacc gcgacggcca gcagaagatc
     7081 gttgatgagc cggtcgcgaa cggcatagaa ggccgccagg agcatccaca cgaccgacag
     7141 cgcgatcacg ccgacactca gggggatgat caccgatcga ggttacgtca tcggctgctc
     7201 gaggtgatca ccaggtaacc ctgcgccgcc atgattccgc ccacgatgat ccacgtgcgg
     7261 gccgggtagt cgactccgta cttgagcgtg tcgagaacca tgacccccag ttgcagcagc
     7321 acgatccagc cgagggtcac cacggccagc tcggcatatt gcagcgcgga ggcatagacc
     7381 cagaagagca gcaccatcgc gcaggcgccg agcccggcca tcgccggcgc gcgatgagcg
     7441 acccattcct tggcggcata cgcggcgacc agatcgatga gggcgagggc cgtcatggcc
     7501 agcgtggcgt tgacgaccag cggccaggat gccggaagcc gaagcatcgg cagggtgtcc
     7561 atcgcgctca cttctgcccc tcgatagtcg ttgccaggtc cgcaggatca gaggccccgc
     7621 ccaccgttct gatacgcggg cggcgtgcac cggcatacgg gtgcggggac ggcatacggt
     7681 ggagtcccga cgaaacatgc ccgccccgat cgacaccccg cgaaggattt ctcgtgaccg
     7741 acccgcgcta ccagcccgcc cccgaggtcg aactgaccca cccggagtgg agcaagaacg
     7801 cgacgatcta ccagatcaac acccgacagt tctccgagga gggcacgctg cgcgcggcgg
     7861 ccgagcaact gccgcggatc aaagatctcg gcgtcgacat cgtttggttg atgccggtca
     7921 acccgatcgg ggaggtcaac cgcaaggggt cgctgggcag cccgtatgcc gtgcaggact
     7981 acttcgccgt caaccccgac ctcggcaccc tccaggatct gaaagacttt gtcgcacaag
     8041 cgcattcgct cggtctctat gtcatcctgg actgggtggc caaccacacc gcgtgggaca
     8101 acgtctgggt gagcgagcac cccgagtggt atgcccggga ctggaaggac gagttcaccc
     8161 cgaccccctg gtgggactgg gacgacatca tcgacctcga ctactcggtc cccgagctgc
     8221 gcgccgagat gacgagggcg ctgctgtatt gggtgagcga ggccgacatc gacggctacc
     8281 ggtgcgatgt cgccggcttc gtgccgaatg acttctggga gaacgcccgt cgcgagatgg
     8341 aggcgatcaa gccggtcttc ctgctggccg agtgggaggc ccgcgacctg cacgtgcgcg
     8401 ccttcgacat gtcgtatgcg tggtcgtgga acgacaccat gcaccacatc gcccagggca
     8461 aggcggacgt cgagcacctc tacatctatt acgcctggaa cgccaaggcc tacccccgcg
     8521 acgcgatgcg gatgatgttc gtgtccaacc acgacaagaa ctcgtgggac ggaaccgagc
     8581 acgagatgtt cggcgatgcg ctcgaagcgg ccatcgtgct gtcggtcatc ggcgagggca
     8641 tgccgctgat ctacaacggg caggaggccg gcaacgacaa gcgcctgaag ttcttcgaga
     8701 aggacccgat cgtctggcgc gagcacccga tggcggactt ctaccgcgat ctgttcgggt
     8761 tgaagaagtc cctgtcctgc ctgtggaacg gcgcctgggg agcgcggatg gtcaatatcc
     8821 gcaacaccgc gtacaagcag gtcctcagtt tcgtccggca ggacgacacc agccgcgtct
     8881 tctgcatcct caacctctcc cccacggagc agaccgtcac cttcggtccc ggcccgcacc
     8941 atgggcattg gcgcgatcac ggcaccggcg agacgtttga cgtggctgcc gacacgacca
     9001 tgacgctcgg accgtgggcg taccggatcc tggtcggagc ctgattcgct tcgccggcgt
     9061 cacgctcctg tgatgcaacg ggcatacgct ggcgtaatgg acgcccggtc cgaatacgac
     9121 gcggcgatca ccgaacgcga gatcggcgtt cttctcgctg ggcaccggcg cgccctcggg
     9181 gcgttgcgcg cctccgaccg cggcgtggtc ctcgatgtga tccggggaga attgcttgcg
     9241 ggcacccgca ccgagccgac cgacctcgac gccctcgccc gattcgtcgt gttcgcggaa
     9301 gtgcgccggc cggggaagtt gctcgatgcg ctcccagagg ccatcgctga tcgcctccgg
     9361 ctcggggtcg tcctcgcgtt gcccgtcacg gccgacccgc ctcgacgacg cccgatcctc
     9421 gcccacctcg acgacaccag gcctcccgag gtcggcagct gggatctcga gggcgacccg
     9481 atctatcagc acacgatctt ctgcaacatg atgcagcacg tcaccgaccc cggagacgtc
     9541 tggcgcaggc gacagcgtcc ccctggcatc cggtaaagcc gctgcggacc ggatgcccgt
     9601 cagagcaggg cccgagccgg ctcatggctc ggccggacgc gtccctgaac gctcgccaat
     9661 cgcaacaaaa cgcggtgtca ggttcgtaag ctcagtagag gaccgaaagg tatcggggac
     9721 aggcgaaggc tatcccggtt tgaaacgatc cgtcatcaga taagatgatc tgtatacgtt
     9781 tcatgccaca agcgaagtgg ggacgatgtc gacgttgagg ccgggtaggg ccgaggaggc
     9841 cggcctgtcg cgcagcgggt tgtatcgtgc ggcccgcgag gggcggctgg agcggatcgc
     9901 tcgtggcatc tacttgccgg cggatgctcc cgcggcggac tgggactgga tcgaggttgc
     9961 gacccggcgg ccggatgcga cgatctgctt ggagtcggcg ctcgcccatc atgatctgac
    10021 cgatgtgatc ccaaacgtgt tggatgtcgc gatcccgcgc gggtcgcgca caccagcgag
    10081 cacgggtgcg attgcgtggc atcaattcga ccgagccacg gtcgagatcg gccgggagga
    10141 gatcccgatt ccgggcacgg accagtcgat cggcatctac tcgcccgagc ggtcgatcgc
    10201 ggatgccttc cggctgcgtg ccgaggtcgg gtacgagttg ccgcgggaat cgttgaagga
    10261 gtggctgcgc cgcggtggta agcctgctcg gctgatcgag atcgcctctc gtctccctcg
    10321 ggcgaagtcc ccggtcctgc aggctttgga ggcgctggcg tgagtgtggg cgatgaggtg
    10381 ttccgccgtc tccagtccgc ggcacgctcg gcagctgcga aggctgggac gggtgcgccg
    10441 acgcaggagt atctgatccg gcacacgctg gagtcgtttc tggaccgcct cacccgcacc
    10501 gcgcatgccg agggctttgt cctcaagggc gcgattttac tggccgccta cggtgtacgg
    10561 cgcccgacga gagatgccga tgcgaacgcc gtcggtgcgg acgtcaccgc cgatcacctc
    10621 gtcgcagcgg ctcacgacat cgcggcggtc gagaccgatg acggggtggt gttcgatctc
    10681 gaccgatcag cgtgcaggag atccgcgagc acgccgacca cccacagccc ccagcctcct
    10741 ggtcgactgc gccacgcttt acgcccgcac gaacgaccaa ggcaaacgac tcgccaacca
    10801 agcactcacc gacggcatcg agatcagcga agacgaaagg gcgacgatcc gactcgctga
    10861 gccctttgag gttccccccg gaccgtggag gcatcgacaa tgaggattga gatgccagag
    10921 atcaggaaga agtacgaccg ggagttccgt g
//
LOCUS       DAOPJG010000113        10856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6353_length_10856_cov_8.143690, whole genome shotgun sequence.
ACCESSION   DAOPJG010000113 DAOPJG010000000
VERSION     DAOPJG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6353_length_10856_cov_8.143690"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..961)
                     /locus_tag="PKU75_10790"
     CDS             complement(164..961)
                     /locus_tag="PKU75_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPF81410.1"
                     /translation="MRETVRALATEDGALLRELVPPRGRGILPIVGVGPHRDVAGLAD
                     ELEAIAAQPQSQLAREVALLTRPAPRVIRSLEAGTLGRDAARAVHRIWQLTLAESWPV
                     LERELRMETVRRASAVGAVGWASTIGALHPSVSWLAGERTSIVLDKPVRVHEAMAADG
                     TLTLIPSAYLIDEPLVTAGTRDHGIFYPLPGDPERRAGGDATARLLGATRARVLDGLA
                     GPITTSGLARRLDLPLSTVSTHLSVLHEAGLVDRQRAGRSVVWERVG"
     gene            complement(1086..1931)
                     /locus_tag="PKU75_10795"
     CDS             complement(1086..1931)
                     /locus_tag="PKU75_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81411.1"
                     /translation="MSEGYRGRYGREESYSDDVIFGADASRKDPRPQDPGPQEGPGRH
                     TRPGARGSAVSSGPFVEVETVTKTYGSGETAVTALHEVSLSVPPGEFLAIQGRSGSGK
                     TTLLNVLGGLDRADSGRIRVGGADVTAMSDDELLALRRDQVAYIFQGFGLLPILTAAE
                     NVGIPLRLKGIDPTDREERVRAALAHVGLEQHPNQTPAELSGGQQQRVAIARALVGGP
                     GLLLADEPTGQLDSATAREIMDLIGRLVRETGMTAIVTTHDPILLSLADRVLEITDGR
                     LGESP"
     gene            complement(1924..2844)
                     /locus_tag="PKU75_10800"
     CDS             complement(1924..2844)
                     /locus_tag="PKU75_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81412.1"
                     /translation="MLPRSPPPRPGGRIRRSCDNLVRIFKSEGVEVVALQGLDLTVRQ
                     GELIAVVGASGSGKTTLLNILSGLDIPSAGVARVAGSDLLAMSSRDRVTYRRETVGFL
                     FQQATANLLPYLTASENIEMPMALARMPKGERRDRSRELLELLGIPQVADRPLAAMSG
                     GQQQRVALAVALANEPRVLLADEPTGELDHQSSEEVFAAIERANATYGTTVVVVTHDP
                     LVSEHVQRTVGIRDGRTSSEVLRRTAIDEHGGESVIAEEYAVLDRAGRLQLPREFTTT
                     LEMKDRVRLDLESDHIGVWPDRPGREAGDE"
     gene            complement(2848..5658)
                     /locus_tag="PKU75_10805"
     CDS             complement(2848..5658)
                     /locus_tag="PKU75_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81413.1"
                     /translation="MNAPLTLFALWRRQLRDRGPGLLLALLTLATTGYLVAVPGLERA
                     AYDRAIGDRLGDAPRTTRDLQLTYSAGRVQPMGTYGGEALDAPTSPEKPMAATAAKVM
                     GPDVAALLAPPLFSARSVEFVVQTPQGDFFHNPGREGVVRAQAGLDKRVTWDRGQLGP
                     STKTVTYRDKDPYSTDESGNPVTMVRKVPVIPVALATSVADEWDAKIGDRFQLTALRH
                     VPYESPFPFQVELAATYTPTNPTDPFWSDDARLVKSNVVVNGEGGTSSSVAFLIPPET
                     IPALGQAMEAQPEGMPRMNPTDYARGLNYTWRYGIDPERVRKAAAAETLRTGVSRLRT
                     GGPAWGEAKPGVVTDVVELLDAHDRAVAGTRALIAFVTIALLALGTLAAGLVAAVLAG
                     RRAASARVLAARGGSPGQIRRLVAGEVLWWTLPAAVVAALVAWRVAGRPTAASALAAI
                     PVLVALGTALVLATRAARTGPERSAGLTAPATRVLAELAVLAGAYFAVTTVQARGGEI
                     VAGQVDWYAAMAPVLLALAAAAVVLRAFPLLLRGAARLASRGRAAMGFLGLARASRGG
                     SGALLPLTGLVVAATVATFLASLSASIDQGRAAAAYQATGADLRVDAVRVDPGDLDAL
                     RAVPGVRSVVPALVSTGERVTGRAGSGVQAVTVIGVDVPAYAASLAGTPLAFSPSGAG
                     GSVDALTGLTSEPLRPGTSLLEVRVNGDFRKLTTTRVVPALRRATTTATTVPVVMLPW
                     AELLAGQPYLQPNTAFVTGTPEAMARIAADPPVGLAERVIDRRALAAHTAGQALPTLV
                     RGLGIAGFALAGLFSVLALGVTLARTRPERVDALMRMRVLGLRRRRDWLLGALEVVPA
                     ACAAILAGVAIGIVLPGVMAKAIDLGPFVGGTPHPPVHTSWLGALLVAAALLLLTLLA
                     VIVDGIRTRSRSLAAHLRGDHR"
     gene            complement(5665..8766)
                     /locus_tag="PKU75_10810"
     CDS             complement(5665..8766)
                     /locus_tag="PKU75_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF81414.1"
                     /translation="MEGAAGVLTVGMRRLHHHRAALVVLALTALTSLLVLGTLQLLSR
                     VVTDRAAEGALVAAPVAERTISAVRSVDKDEVAAARDVVAQAQAAVPGSRLSEVFEAI
                     SRGIEGRDPTDRAGLAVLADLPRWTTLAAGAWPVAGSDPIQVVLPEAAAAAGGWQVGD
                     RMDLPDLITDNAPPLHVVVTGIVTAKDPLDAVWLDLPLAQTGTRTGAGFTSYGPFFAD
                     GGDLETVIPAATARWRIALPSTGLTRALAPVRQGQVEYATRLLTESPDLKGAQVRTDA
                     PQLYAEASLISERTRIALLTPTILLMLLGSVALTMAGLQLAGLRAQETQLLRSRGAST
                     SQIVGLAAIDAGMIAIGAFVVAWLTVPLLARPLARLGGLDIPTPAWSDAIRWRGLVLP
                     LLVAALAAAAIVAITAARSGQLRLATQRSRRGQLLARLAGAGLDLVLIGFGVLGLLQL
                     RRYDVTTPRADPLTLAAPALVLAGLAVLGLRLIPWLARVAAAAAGRRSGLTLAWGSWQ
                     VARRLAGQAGAILLIFLSVAMGALGVAQAATLGRAISDQTHFAAGSPLRVTTGSELTG
                     KAGLRERYAAIAGGTDRVMAAHVGSIGVGSAKEVTMLAVDTHAAGVAFTPRADVAPDW
                     QRTLEKLAVPVAGLDIPAGARELAAHVQFELDEQFEWAMLPARPDFLVALADGEWIQV
                     PGERVTSSGSVRLRADLPQVVGGWPAGTRLIGITMAPASTYLPPEAVASAGLASADPA
                     APVRLDGVDMRVAGKPEPDRWIGGALFLPLGDHPAPAPVPVLVTRDLLTEARLDAGRD
                     FSIEVSGTAVPVHVVSVVDAIPGVPTPRRALMLHLEALADPQQPGQENLGPPVAQQWW
                     LAPADVAAARAALAKEPRLATALVDQEEVEEQRRANPVNAGMRAALRLVTASALVLAS
                     LGFAAATAGLALARHREGAILLALGTPPGHIRRAVVGERLVVLVLASLIGVLAGLAAA
                     RVIVPLVVGSDGYEQIPPVAVDLDVLRLLVFVGVVLAVLALISILVIGRTARDLADVL
                     RGR"
     gene            complement(8966..10630)
                     /locus_tag="PKU75_10815"
     CDS             complement(8966..10630)
                     /locus_tag="PKU75_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HPF81415.1"
                     /translation="MTEFPLSSPAQVGRPVDAYPDKIDGAVLKIAGVVVLGAIMSILD
                     ITVVNVALPELQTTFTGTDNPLSYATVAWTVTAYTLALATVIPLTGWAADRFGTKRLY
                     MLAIALFTLGSVLCAAATSINLLILFRVLQGLGGGLLMPLGMTIMTRAAGPHRMGRLM
                     AILGIPMLLGPILGPILGGYLIDAVSWHWIFLINLPLGIIALLYAAWALPKDTPEPSE
                     SFDFLGMAMMSPGLAMFLYGISSIPEAGTFFSAKVMGWGTAGLLLLIAFVIYSFKPKH
                     PLLDFRLFQNRNLTVATITMFIFGAAFFGGLLLVPTYFQQVRGEDALHAGLLVAPQGI
                     GAMVTMPIAGALADKKPVGRIVPFGLLAIIVGMFLLSRVDGDTSYWGYIIPVLFLLGL
                     GMGGTMMPIMTSAIKTLTAHQVARGSTLLNITQQIASSVGVAIMSVVLTNGLKNDALV
                     SQAAGFREASAGVTDPAQIQQLLPQFPKVAEILAAAGNDQAAGAAKLAAAAADAAGKV
                     FGNSFLVAAFLVLLTLVPAFFLPRDHEESHLTDDEDDAPAAPVFVH"
BASE COUNT         1636 a         3782 c         4048 g         1390 t
ORIGIN      
        1 ccttcaaaca gggccgctgg gtcgtcatcg ccgaccagaa caccccactg gccagctgac
       61 ccggcccccg ggacgagcgc tcggctgccg gtgaccggga agccggcagc cgagtcaccc
      121 catccggagc cactccacac gagtcatccc cgagcggatc ggctcagccc acccgctccc
      181 atacgacact gcgcccggcg cgctgccggt cgaccaggcc ggcctcgtgc aggaccgaca
      241 ggtgggtcga gacggtagag aggggcaggt cgaggcgccg ggcgagtccg ctggtggtga
      301 tcgggccggc cagcccgtcg agcacgcggg cccgggtggc gcccagcagc cgtgccgtgg
      361 cgtcgccgcc tgctcggcgc tcggggtccc ccgggagggg atagaagatg ccgtggtcgc
      421 gggtgccggc ggtgaccagg ggttcgtcga tgaggtaggc cgacgggatc aacgtcagcg
      481 tgccgtcggc cgccatggcc tcgtggaccc gcacgggttt gtccagcacg atgctcgtgc
      541 gctcgccggc gagccacgag acgctcgggt gcagggcacc gatcgtgctc gcccagccga
      601 cggctccgac agcgctcgcc cggcggacgg tctccatccg cagctcccgc tccagaacgg
      661 gccacgactc ggccaaggtc agctgccaga ttcggtgcac ggctcgggcc gcatcacgtc
      721 ccagggtgcc cgcctcgagg ctgcgtatga cgcgcggcgc cggacgcgtc agcaatgcca
      781 cctctcgcgc aagctggctc tgcggttgcg ccgcgatggc ctccagttcg tcggctaggc
      841 ccgccacgtc gcgatgcgga cctacaccga cgatggggag aatcccccgg cctcggggtg
      901 gcaccagttc gcgcagcagc gcgccgtcct ccgtggcgag ggcacgcacc gtttcgcgca
      961 cggggcgcgc cgggtcgggt gccgccggcc ggatgctgcc agccggagca gtgccatcag
     1021 ctggttgacc ggagatatgt ggaagcgggc ggaggccagg ggcgcgactg cgacgtcgag
     1081 gaccttcacg gcgactcccc caaccgcccg tcggtgatct cgaggacccg gtcggccagg
     1141 ctgagcagga tcgggtcgtg ggtggtgacg atcgcggtca tgccggtctc gcgcaccaga
     1201 cggccgatga ggtccatgat ctcgcgagcc gtcgccgagt cgagctggcc ggtgggctcg
     1261 tccgccagca gaagccccgg cccgccgacc agggcgcggg cgatcgccac ccgctgctgc
     1321 tggccaccgg acagttcggc gggtgtctgg ttgggatgct gctccagacc gacgtgggcc
     1381 agcgccgcgc gcacccgctc ctcccggtcc gtcgggtcga ttcccttcag ccgcaacggg
     1441 atcccgacgt tctcggcggc ggtcaggatc ggcagcagcc cgaacccctg gaagatgtac
     1501 gcgacctgat cgcgacggag cgcgagcagc tcatcgtcgg acatcgcggt gacgtcggct
     1561 ccgccgaccc ggatgcgccc gctgtcggct cggtccagac cgccgagcac attgagcagc
     1621 gtcgtcttgc ccgagcccga ccggccctgg atcgcgagga actcgcccgg cgggaccgat
     1681 agcgacacct cgtgcaacgc cgtcaccgcc gtctcgccgc tgccatacgt cttcgtgacg
     1741 gtctcgacct ccacgaacgg gccggaggac accgctgacc cgcgcgcacc cggccgcgtg
     1801 tgcctgcccg gcccctcctg cggccccgga tcctgcggcc ggggatcctt ccgactcgcg
     1861 tccgcgccga agatgacgtc gtcgctgtaa ctctcctcgc ggccataccg tccgcggtag
     1921 ccctcactca tcgccggcct cccgtcccgg ccggtccggc cagaccccga tgtggtcgct
     1981 ctccaggtcg aggcgcaccc ggtccttcat ctccagggtg gtggtgaact cccgcggcag
     2041 ctgcaaccga ccggcccggt cgaggacggc atactcctcg gcgatcaccg actcgccgcc
     2101 gtgctcgtcg atcgcggtgc ggcgcaacac ttccgaggag gtgcgcccgt cccggatgcc
     2161 gaccgtccgc tgcacgtgct cactcaccag tgggtcgtgc gtcaccacga cgaccgtcgt
     2221 cccataggtc gcattggctc gctcgatcgc ggcgaagacc tcctccgagc tctggtggtc
     2281 cagctcaccc gtgggctcgt ccgcgagcag cacccggggc tcgttggcga gcgcgacggc
     2341 cagggcgacc cgctgctgct ggccgccgga catcgctgcg agcgggcggt cggcgacctg
     2401 cggtatgccg agcagctcca gcagctcacg ggaccggtcc cggcgttcac ctttcggcat
     2461 ccgcgcgagc gccatcggca tctcgatgtt ctcgctcgcg gtcaggtagg gcagcagatt
     2521 ggctgtcgct tgctggaaga ggaagccgac ggtctcgcgg cgataggtca cgcggtcccg
     2581 ggaggacatg gcgagaaggt ccgagccggc cacccgggcc acgccggcac tggggatgtc
     2641 gagtccggag aggatgttga ggagcgtcgt cttgcccgac ccggacgccc cgacgaccgc
     2701 gatgagctcg ccctgccgca cggtcaggtc gaggccctgc aaggcgacga cctccacgcc
     2761 ttcggacttg aagatgcgaa cgaggttgtc gcaggatctc cggatcctcc cgcccggtcg
     2821 cggcggaggg gatcgtggca gcagtggtca tcggtggtct cctcgcaggt gggcggcgag
     2881 cgatcgggaa cgagtacgga tgccgtcgac gatgacggcg agcagggtga ggagaaggag
     2941 ggcggctgcc accagaagag cccccagcca ggaggtgtgc acaggcgggt ggggcgtgcc
     3001 cccgacgaag ggccccagat cgatggcctt ggccatcacc cccggcagga cgatcccgat
     3061 ggcgacgccg gcgaggatgg ccgcgcaggc tgcggggacg acctcgaggg caccgagaag
     3121 ccagtcgcgg cgccgccgca acccgaggac gcgcatccgc atgagcgcgt cgacccgctc
     3181 gggccgggtg cgggcgagcg tgacaccaag ggccaggacg ctgaacaggc cggccagcgc
     3241 gaaccccgcg atgcccagtc cacgcaccag cgtgggcagg gcctgcccag ccgtatgggc
     3301 agcgagggca cgccggtcga tcacccgctc ggcgagtccc accggcgggt cggcggcgat
     3361 ccgcgccatg gcctcggggg tgccggtgac gaaggccgtg ttgggctgca gatagggctg
     3421 tcctgcaagg agttccgccc acggcagcat gacgacgggg accgtggttg cggtggtggt
     3481 ggcgcgccgc agcgccggca ccacccgcgt cgtcgtgagc ttgcggaagt ccccgttcac
     3541 cctcacttcc agcaggctgg tgcccggccg cagcggttcg ctggtcaaac ccgtcagcgc
     3601 gtccacgctg ccgcccgcgc cggacggcga gaaggcgagc ggcgtgccgg ccaggctcgc
     3661 ggcatacgca ggcacgtcga ccccgatgac ggtgacagcc tgcactcccg accccgcgcg
     3721 gccggtcacg cgctcccccg tcgagaccag cgcgggcacc acggacctca ccccggggac
     3781 ggcccgcaga gcgtcgagat caccgggatc gacccggacg gcatcgacgc gcaggtcggc
     3841 gccggtggct tggtatgccg cggcggcccg gccctggtcg atgctcgcgg agaggctcgc
     3901 caggaaggtc gccaccgtgg ccgccacgac caacccggtc agcggcagca gcgcgccgga
     3961 gccgccccgg gacgcccgcg ccagcccgag gaagcccatc gccgcccggc cccgggaggc
     4021 cagccgggcg gcgccgcgca agagaagggg aaacgctcgc aggacgacgg cggcggccgc
     4081 cagcgcgagg aggacgggcg ccatcgcggc ataccaatcc acttgccccg caacgatttc
     4141 cccgccacgc gcttgcaccg tggtgacggc gaaataggca ccggcgagca cggcgagctc
     4201 ggcaaggaca cgggtcgccg gcgccgtcag gccggccgaa cgctcaggcc cggtccgggc
     4261 ggcccgcgtg gccaggacca gtgcggtccc gagagcgacg agcaccggga tcgccgccaa
     4321 cgcggatgcc gcggtgggac ggccggcgac gcgccaggcg accaacgccg cgacgactgc
     4381 ggccggaagc gtccaccaca ggacctcgcc cgcgaccagc cgccggatct ggccgggcga
     4441 gccacctcgg gccgccagca cgcgagcgga cgccgcgcgc cggccggcga gaacggctgc
     4501 gaccaggccc gcggcgagcg tgccgagcgc gaggagggcg atggtcacga aggcgatcag
     4561 ggcgcgagtg ccggcgaccg cacggtcgtg ggcatcgagc agttcgacga cgtcagtcac
     4621 gactcccggc ttggcctccc cccaggcggg accacccgtg cgcagccggc tcacgccggt
     4681 gcgcagcgtt tcggcggctg ccgccttccg cacccgttcg gggtcgattc cgtagcgcca
     4741 ggtgtagttc aggccacggg cgtagtcggt cgggttcatt cgcggcatac cctccggctg
     4801 ggcctccatc gcctgcccga gggccggaat cgtctcgggc gggatgagga aggccacgga
     4861 cgaggaggtg ccgccctcgc cgttgacgac gacgttcgac ttcacgagtc gggcgtcatc
     4921 ggaccagaag gggtccgtcg gattcgtggg ggtgtaggtc gcggccagct cgacctggaa
     4981 ggggaacggg ctctcgtacg gcacgtggcg gagggcggtg agctggaacc ggtccccgat
     5041 cttggcgtcc cactcgtccg cgaccgatgt ggccaacgcg acggggatga ccgggacctt
     5101 gcgcaccatc gtcaccgggt tgccggactc gtcggtgctg tagggatcct tgtcccgata
     5161 ggtcaccgtt ttcgtggatg gacccaactg cccgcggtcc caggtgaccc gcttgtcgag
     5221 cccggcctgg gcacgcacga cgccctctct gccggggttg tggaagaagt cgccctgcgg
     5281 tgtctggacg acgaactcga cgctgcgcgc agagaagagc ggcggcgcga gcagggcggc
     5341 gacgtcgggc cccatgacct tcgccgccgt ggccgccatc ggcttctccg ggctggtcgg
     5401 cgcgtcgagg gcctcgcccc cataggtgcc catcggttga actcggccgg cggaataggt
     5461 caactggagg tcgcgcgtgg tgcgtggcgc gtcaccgagt cggtccccga tggcgcggtc
     5521 gtatgccgcg cgctccaggc ccggcaccgc caccaggtag cccgtcgtgg ccagggtgag
     5581 cagggccaac agcaggcccg gtccgcggtc gcgcagctgg cgacgccaga gcgcgaacag
     5641 ggtcaacggc gcgttcattc gcgctcaccg gccacgtagc acatcggcca ggtcgcgggc
     5701 ggtgcgcccg atgacgagga tggagatcag ggccaggacg gcgagtacga cgcccacgaa
     5761 gaccagcagg cggagcacgt ccaggtcgac cgcgaccggc gggatctgtt cgtagccgtc
     5821 gctgcccacg acgagcggaa cgatcacccg ggcggctgcc aggcccgcga ggacgccgat
     5881 caggctggcg agcaccagca cgacgagccg ttcgccgacg acggcccgcc ggatgtgccc
     5941 gggcggggtg ccgagagcga gcaggatggc tccttcccgg tggcgggcca gcgccaatcc
     6001 cgctgtggca gcggcgaacc cgaggctggc gagcacgagc gccgaggcgg tcacgaggcg
     6061 gagagccgcc cgcataccgg cgttgaccgg attggctcgg cgctgctcct ccacctcctc
     6121 ctggtcgacg agggcggtcg cgagccgggg ctccttggcc agagcggcgc gcgccgccgc
     6181 cacgtccgcg ggcgcgagcc accactgctg cgcgaccggt gggcccaggt tctcctggcc
     6241 gggctgttgc gggtcggcga gcgcctcgag gtgcagcatc aacgcccgcc ggggggtggg
     6301 cacgccgggg atcgcgtcga ccaccgagac cacgtggacc gggacagccg tgccggacac
     6361 ctcgatgctg aagtcccggc cggcgtcgag ccgggcttcg gtgaggaggt cgcgcgtgac
     6421 gaggacgggc accggagcgg gagccggatg atcccccagg ggcaggaaca gcgcgccgcc
     6481 gatccagcgg tcgggctcgg gcttcccggc aacgcgcatg tccacgccgt cgagccgcac
     6541 gggcgccgcc gggtcggcac tggcgaggcc ggcgctcgcg acggcctccg gggggaggta
     6601 tgtgctggcc ggtgccatgg tgatgccgat cagccgggtg ccggcgggcc agccgcccac
     6661 gacctggggc aggtcggctc gcagccggac cgaaccactc gacgtgactc gttcgccggg
     6721 cacctggatc cactcgccgt cggccaaggc gacgaggaag tccgggcggg ccgggagcat
     6781 ggcccactcg aactgctcgt ccagctcgaa ctgcacgtgg gccgcaagct cccgcgcccc
     6841 ggccgggatg tccaggccgg cgaccggcac cgccagcttc tcgagcgtcc tctgccagtc
     6901 gggcgccaca tccgcgcgcg gggtgaacgc cacgcccgcc gcgtgcgtgt cgacggccag
     6961 catggtcacc tctttcgccg atccgacccc gatcgagccg acatgggcgg ccatcacgcg
     7021 gtcggtcccg ccggcgatgg cggcataccg ttcccgcagc cccgccttgc cggtcagttc
     7081 gctccccgtg gtgacgcgca gcggggagcc ggcggcgaag tgggtctggt cggagatggc
     7141 ccgccccaag gtggcggcct gggcgacgcc gagggcgccc atggcgaccg agaggaagat
     7201 gagcaggatc gcccccgcct gcccggccag gcgacgcgcg acctgccaag acccccacgc
     7261 gagcgtcagg ccggagcggc ggccggccgc ggccgctgcc acgcgggcca gccacgggat
     7321 cagccgcagt ccgagcaccg ccagcccggc gagcaccagc gcgggcgcgg cgagcgtgag
     7381 cgggtcggcc cggggggtgg tcacgtcata ccgccgcagt tgcagcagcc cgagcacgcc
     7441 gaacccgatg aggaccaggt ccagtccggc gccggcgagc cgggcgagga gctgaccccg
     7501 gcgggagcgt tgggtggcca ggcgcagctg gccgctgcgg gcggccgtga tcgcgacgat
     7561 ggcggcggcg gcgagggcgg cgaccagaag cggcaggacc agtccgcgcc agcggatcgc
     7621 gtcggaccag gccggggtgg ggatgtcgag tccgccgagc cgcgccagcg gtcgggcaag
     7681 gagcggcacg gtgagccagg cgacgacgaa tgcgccgatg gcgatcatgc cggcgtcgat
     7741 cgccgcgagg ccgacgatct ggctggtgga ggcgccgcgg gagcgcagca gctgggtctc
     7801 ctgggcgcgc aacccggcca gctgcaggcc ggccatcgtc aaggcgacgc ttccgaggag
     7861 catgagcagg atcgtcggtg tcagcagggc gatgcgggtg cgttcgctga tcaggctcgc
     7921 ctcggcatac agctgcgggg cgtcggtgcg cacctgcgca cccttcaggt ccggactctc
     7981 ggtcagcagc cgggtggcgt actcgacctg gccctgtcgc accggggcga gggcacgggt
     8041 gagcccggtc gacggcaggg cgatgcgcca gcgggccgtc gccgcgggga tcaccgtctc
     8101 gagatcgccc ccgtccgcga agaagggacc gtatgacgtg aagccggccc ctgtgcgggt
     8161 gccggtctgc gcgagcggca gatcgagcca cacggcgtcg agcggatctt tcgcggtgac
     8221 gatgccggtg acgaccacgt gcaggggcgg ggcgttatcc gtgatcaggt cgggcaggtc
     8281 catcctgtcc ccgacctgcc agccccccgc cgcggccgcc gcctccggaa ggaccacctg
     8341 gatcgggtcg ctcccggcga cgggccaggc accggcggcg agcgtggtcc atcggggcag
     8401 gtcggcgagc accgccagcc cggcccggtc ggtggggtcg cggccctcga taccgcggga
     8461 gatcgcctcg aacacctccg acagccgcga tccgggaacc gccgcctgcg cctgcgccac
     8521 cacgtcgcga gcagcggcca cctcgtcttt gtccaccgac cggacggcgg agatggtgcg
     8581 ttcggcgacc ggcgccgcaa cgagcgcgcc ctcggcagcc cggtcggtga cgacccggct
     8641 gagcaattgg agcgtgccca gcacgaggag cgaggtcagc gcggtgaggg caaggacgac
     8701 cagcgccgcg cggtggtggt gcaggcgccg catacccacc gtcaacacgc ccgcggcccc
     8761 ttccatcctt gaagcggcgg cctcccgccc ggcggccgcc tcgtcactct gtcagacctc
     8821 acggacgagg cggttcgccg ggttggcctt gttacccaag agtgacgcgg gcggccggtt
     8881 tgccttgtga cgcggcgacg cccggcggac cgggtgtggt ccgccgggca tcggcgtggg
     8941 aagtcggcgc ggtggcgccg gctcgtcagt ggacgaaaac gggtgccgcg ggggcgtcgt
     9001 cctcgtcgtc ggtgaggtgg ctctcctcgt ggtctcgcgg gaggaagaac gcgggaacga
     9061 gcgtcagcag caccaggaag gccgccacca ggaagctgtt gccgaagacc ttgccggccg
     9121 cgtcggcggc agccgcggca agcttggcgg cgccggccgc ctggtcgttc ccggcggcgg
     9181 cgaggatctc ggcgaccttc gggaactgcg ggagcagctg ctggatctgc gcggggtcgg
     9241 tcactcctgc gctggcctcc cggaagcccg cagcctggct gaccagcgcg tcgttcttca
     9301 acccgttggt gaggacgacg gacatgatcg cgacgccgac cgagctggcg atctgctgcg
     9361 tgatgttgag cagcgtcgag ccccgggcga cctggtgcgc ggtgagggtc ttgatggccg
     9421 aggtcatgat cggcatcatc gtgccgccca tgccgaggcc gaggagaaag agcacgggga
     9481 tgatgtagcc ccagtagctc gtgtcgccat cgacccgcga cagcaggaac atgccgacga
     9541 tgatggcgag cagcccgaag ggcacgatcc ggccgacggg cttcttgtcg gcgagcgcgc
     9601 cggcgatggg catggtcacc atggcgccga tgccctgcgg ggcgacgagc aggcccgcgt
     9661 gcagggcgtc ctcgccgcgg acctgctgga agtaggtcgg caccagcagc aggccgccga
     9721 agaaggccgc accgaagatg aacatcgtga tggtggcgac cgtcaggttg cggttctgga
     9781 acaggcggaa gtcgaggagc gggtgcttgg gcttgaagct gtagatcacg aaagcgatga
     9841 gcagcagcag gccggcggtg ccccagccca tgaccttcgc ggagaagaag gtgcccgcct
     9901 cagggatgga ggagatgccg tagaggaaca tcgccagccc ggggctcatc atcgccatgc
     9961 cgaggaagtc gaaggactcg ctgggctccg gggtgtcctt gggcagcgcc caggcggcat
    10021 acaggagggc gatgatgccg agggggaggt tgatgaggaa gatccagtgc cagctgacgg
    10081 cgtcgatgag gtagccaccg aggatcggcc cgaggatggg cccgagcagc atggggatgc
    10141 cgaggatcgc catgaggcgg cccatgcggt gcgggccggc tgccctggtc atgatggtca
    10201 tgcccaacgg catgagcaga ccgccgccga ggccctgcag gacgcggaag aggatgagca
    10261 agttgatgct cgtcgccgcc gcgcacagca ccgagccgag ggtgaacagc gcgatggcca
    10321 gcatgtagag gcgcttggtg ccgaaccggt ccgcggccca cccggtcagc gggatcaccg
    10381 tcgccagggc gagggtgtag gccgtcaccg tccacgcgac ggtggcatag gacagcggat
    10441 tgtcggtgcc ggtgaaggtc gtctgcagtt ccgggagggc gacgttgacg acggtgatgt
    10501 cgaggatcga catgatggcc ccgagcacga cgacgccggc gatcttcagg accgccccgt
    10561 cgatcttgtc ggggtaggcg tcgaccgggc ggccgacctg ggctggggag gacaaaggga
    10621 actccgtcat gttcaagagg tgtcccgccc cgggcaaaca cgacccatca cgtcgatcgc
    10681 cggcgaggcc gatcggaggc gtgcatggat ttgggaggca gagcgggtgt cggcgacagc
    10741 gccgcagtcc tcaagggtag cagtttgaac gttcactcat ctaccggatt ccgcgccacc
    10801 cggccccacc gcccacgccc ggtgcacacc agctatcagt aggcacccgc tcctgc
//
LOCUS       DAOPJG010000114        10770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6414_length_10770_cov_8.500887, whole genome shotgun sequence.
ACCESSION   DAOPJG010000114 DAOPJG010000000
VERSION     DAOPJG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_10770_cov_8.500887"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..931)
                     /locus_tag="PKU75_10820"
     CDS             complement(197..931)
                     /locus_tag="PKU75_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPF81416.1"
                     /translation="MSQPTSPGRAPEGVVVIIPALHPEPGFAGFAAQLAASFTEVLVV
                     DDGSGADYADIFARIAHAPGCRVLTHTVNRGKGAALKTAYADLLGRRPARVIVTADCD
                     GQHTAEGIAAVAAAVQAAGPEQVLVLGVRRFSGPGIPFFSRLGNGFTTGVIRMRYGQR
                     LPDTQTGLRGFPLALAEPMSRVEGERFEYEMSVLIWALAQHIPILEVPIATVYHDAKN
                     SQSHFRPLRDSLMIYRTILRQARSRK"
     gene            1049..2011
                     /locus_tag="PKU75_10825"
     CDS             1049..2011
                     /locus_tag="PKU75_10825"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81417.1"
                     /translation="MSDLTMPLTVRTARRRTTATARRTGLITIVTGGGPIGGHDHRCA
                     STAMAEALPDACGVVECRVVEATAGTRLAPGLAGAGLVVPSVPGELLAPLAALCEWLE
                     IPYAGASPRASTLAADAELAALALDRAGLDVVPGLAVDAAGAAGLDYLDPVRIRPAGR
                     PGPFGRSIATTRASLRDGLHLALALDERAYVEEIPQGMAVMVPVLDDGTGNLLTGAVH
                     EIAPGTLFAADRPAAPGDDWHDAHLSPTDGALVRRAARAAYRALGMRGVVSVEIILDG
                     RRALIGAIDPAPAQRPHGLTARQFAASGTAYPQLVAAVAHAARG"
     gene            2056..3363
                     /locus_tag="PKU75_10830"
     CDS             2056..3363
                     /locus_tag="PKU75_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPF81418.1"
                     /translation="MTTPGARTPTPAPATIDRARIGMPPRWVARALATAACLYGLFAV
                     VTGQMYDNTAWVLLFGIIGPAVALLGADRSPGLGFVVFAGSLILAGYGGGWVSQVAIP
                     IGVILLFACTAFGSRTTVVLTGIAMALVPVVGFVLWAFGNYGISSIDAFLRQLSWAQV
                     PSVIARAFRNFPGWAALVYLVIVVLPWGAGLAWRSVAGSRRAAVAAVEERDQANRETA
                     YAQEIAHLRAGQTQLARDVHDVVGHSLAVILAQAQSAQYLGDDEVDKMRETLGHVADS
                     ARRSLGDVRGVLSGTREATSAASAGLDSLIAGVRNAGNEVVERVDGTPRPLPPELEQV
                     AYRVVQEMLTNALKHGREGGPVWVRQVWPAQPEGHLLLEVHNLMDTGRTASGHGIGLG
                     SMRDRLASVGGSLHVETEDTVSGPMYAARADLPLRAAEIAEEG"
     gene            3366..4028
                     /locus_tag="PKU75_10835"
     CDS             3366..4028
                     /locus_tag="PKU75_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF81419.1"
                     /translation="MNDIRVLLVDDQELFRAGIARILEAQEGITVAGEAGDGAEAVAR
                     VDEIAPDVVLMDIRMPVMDGVEATRQIFAPARVAKREKPVRVVVLTTFNLDDRAATAI
                     RYGASGFLLKDATPVMLREAIHTVYAGNAVLAPSDLSTLLDGQFRARKPLPADFHSLT
                     EKEREVLAFVARGLSNAEIAAAMFASESTIKTHLGAVLRKLGLRDRVQVVVFAHEHGL
                     SE"
     gene            4083..4589
                     /locus_tag="PKU75_10840"
     CDS             4083..4589
                     /locus_tag="PKU75_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81420.1"
                     /translation="MGRLRQAAHAAGQLTKSGLRRPDSPTAPAPPIARRSAAETAAAG
                     PAQTHDVASALADAMRIDGALAVALADSRSGEVLAADGDVDGMQLATAVAGNARVVRA
                     KLDTLHDLGMDERIEDILITLDSQYHIIRTFAKRDGLFLYLVLDKPLANLAMARFKVA
                     TLERDLEL"
     gene            4610..4999
                     /locus_tag="PKU75_10845"
     CDS             4610..4999
                     /locus_tag="PKU75_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPF81421.1"
                     /translation="MDEGSTPADSSENGRLGVARDRLHAARTETLERLTGLLGEIAGI
                     VAASEGSNADDEHDPEGATIAYDRAQTDALAQQARHQLAEIDAALARVENGTYGRCTV
                     CGERIPEARLEARPTATTHVEHAAPRR"
     gene            complement(5033..5458)
                     /locus_tag="PKU75_10850"
     CDS             complement(5033..5458)
                     /locus_tag="PKU75_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81422.1"
                     /translation="MSEREEIPSPLHARQRPGEDTDAARLIDQMVPPQLRGVPALAIL
                     WLDDEGGVVQPIMIDDVGDEVLQDALPAGLVTFAAMVGAADGRIVLARVRPGLPFLTA
                     ADRAWRTYAETLFGELLTEVLLVTPDLVRRVAAPERRTA"
     gene            5627..6679
                     /locus_tag="PKU75_10855"
     CDS             5627..6679
                     /locus_tag="PKU75_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPF81423.1"
                     /translation="MKALVFHGPGERAWEEVADPAIVEPTDALVRIDTTTICGTDLHI
                     LKGDVPAVTPGRVLGHEGVGTVLETGTGCTRVRAGQRVIVSCICKCGTCGYCKQGMPS
                     HCQTLGGIGWVFGHLIDGTQAEYVRVPFADNGLVPVPPGVSDEQAVMLSDILPTGYEI
                     GVQYGAVGAGDVVAVVGAGPVGLAAIMTARPLGASRIIAVDGDPFRLEAACAFGATDV
                     IDIGAVDDVAAALRRMSPDGLGVDVAIEAVGVPATFTTCLDAIRPGGVVANVGVHGKP
                     VEFPIQRAWIDNITIRTGLVNATTTEDLLGLIAAHEIAPERFATHHFKLAQIMDAYDV
                     FAAAADHHALKVVLTA"
     gene            complement(6741..8183)
                     /locus_tag="PKU75_10860"
     CDS             complement(6741..8183)
                     /locus_tag="PKU75_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPF81424.1"
                     /translation="MADLEFFGHIVDNEEVESIDGKRFDVWNPWKREVWAQAAEGGEK
                     DADRAITSARKAFDEGPWPRMGRVERAKMIHRLADLMEARADEIATLDSTNMAKPFEQ
                     AKHDVARSVWNFRFFADHQRDSVGEVYPMDSGHHSYSEFGPAGVVAAISPWNFPLMMA
                     SWKIAPAIAWGNTCIIKPSEDTPASVTYLGGLAAEAGFPPGVLNVLNGYGHPAGSAIT
                     GDDRVDRVTFTGSSTTGKAVMASAATHLAPVSLELGGKGANIVFADADIDNAVYWAVE
                     AIFRNAGQICLAGSRLFVQDAIYDEFMKRFVDAAEALTIGDPFDPATNFSSLASKRHF
                     DKVVSYVEGARDLGGTFHTGGIAEDGSWVVKPTIIDGLALDAAPYCEEIFGPVCVVNR
                     FETEEEAVHLANDTRYGLNAMLFTENLSRAHRVASKLRAGTVWVNCFFIRDLRTPFGG
                     VGDSGVGREGGNFSREFFTEPKAVVMQITS"
     gene            complement(8333..8533)
                     /locus_tag="PKU75_10865"
     CDS             complement(8333..8533)
                     /locus_tag="PKU75_10865"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HPF81425.1"
                     /translation="MPFIDVTLAEGRSPEQLRTLITKLTDAAVEAVGAPRESIRVVLR
                     EVPPTHWAAGDVTIAERRAARP"
     gene            complement(8538..9374)
                     /locus_tag="PKU75_10870"
     CDS             complement(8538..9374)
                     /locus_tag="PKU75_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPF81426.1"
                     /translation="MTDTTTDAPAYGTAPEGGWTVESIAAELLRCEDERIERPPFTDE
                     WPELDLATGYEIQDLNLQKRLDRGEKLIGVKLGLTSRAKQQRMGVHFPFVAWLTDAMI
                     LPTGDPVPQDKLIHPRIEPEIVFIMGDRLEGPGVTNAQAMAAVESVWGGAEVIDSRYK
                     DFRFTAGDVAADNASSGAFVTGPVGLSPSELDLALEGVLVEVGGEIVDSATGAAVQGH
                     PGEALALAANDLASRGHAIEAGWIVLTGGMTDAVFAPPGSSIACHFTNLGSVFLNGGE
                     AG"
     gene            complement(9376..10515)
                     /gene="dmpG"
                     /locus_tag="PKU75_10875"
     CDS             complement(9376..10515)
                     /gene="dmpG"
                     /locus_tag="PKU75_10875"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HPF81427.1"
                     /translation="MTIRPEDAPKVDHIVTESEVLENLPKAGGETDLRITDSTLRDGS
                     HAMAHQYTEEQVRGVVSALDRAGVQVIEVSHGDGLGGSSFNYGFSKVDEFDLIKAAVE
                     EATQAKIAILMLPGLGTVHHLKKAHEMGASVARIATHCTEADVSIQHFGEARKLGMET
                     VGFLMLSHKASPAKLAEQARIMVDAGAQCVYVVDSAGALVLSDAQERVQAVIDEIGPD
                     GAQVGFHGHQNLSLGIANSVLAYQAGAKQIDGALCALGAGAGNSPTEVLAATFERMGI
                     KTGVDLGEVLSAAEDVVRPFIPRLPWMDRASITQGYAGVYSSFLLHAERAAARYDVPA
                     HAILQKVGEYGYVGGQEDMIIDIALELQQEKERMALGDLAAAGVR"
     gene            complement(10530..>10770)
                     /locus_tag="PKU75_10880"
     CDS             complement(10530..>10770)
                     /locus_tag="PKU75_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81428.1"
                     /translation="DVADAGCGGGDDVFVPIEVKGRGDYLPDYAGNLDIITAAAARVG
                     DIMVAHRLGVETSWKSSADLGDAPDAIVETEKAGV"
BASE COUNT         1593 a         3712 c         3774 g         1691 t
ORIGIN      
        1 cgcctcggcg atgacgacat tctcggaggc cgcggaggcg gacggcgcgc agacggcgcc
       61 gagggccagc acgggggtag cggccatgat tagccgcagg gtttgctggg agtcacggtg
      121 gtgctcctgg gacgaaccgg tgggggatcg gcgtcacccg acgagtcaaa ggtgtccggc
      181 gcaccacctg cgcgggtcat tttcgagaac gtgcctggcg caggatggtc cgatagatca
      241 tcagcgagtc gcgcagcggc cggaagtggg actggctgtt cttcgcgtcg tgatagacgg
      301 tcgcgatcgg cacctcgagg atcggaatgt gctgggcgag cgcccagatc agcacgctca
      361 tctcgtattc gaagcgctcc ccctcgaccc ggctcatcgg ttcggcgagc gcgagcggga
      421 acccgcgcag cccggtctgc gtgtcgggca ggcgctggcc gtaccgcatc cggatcaccc
      481 cggtcgtgaa gccgttgccg aggcggctga agaaggggat gcccggaccg gagaaccggc
      541 ggaccccgag cacgagaacc tgttcaggcc cggccgcctg caccgccgcc gccacggcgg
      601 cgatgccttc ggcggtatgc tgcccgtcgc agtccgcggt cacgatcacc cgggccggcc
      661 gtcgcccaag cagatcggca tatgcggtct tcagggccgc tcccttgccg cggttgacgg
      721 tgtgggtcag gacccggcac cccggtgcgt gggcgatccg ggcgaagatg tcggcgtagt
      781 cggcgcccga gccgtcgtcg accacgagaa cctcggtgaa actcgccgcc aactgggccg
      841 cgaagccggc gaatccgggc tcgggatgca gtgccgggat gatgacgacc acgccttccg
      901 gcgcgcggcc cggcgaggtc ggctgcgaca ccttcgcatg ttcccacgca cgatgccgcg
      961 aaccggggat tgccgctgtc ctatcggcca gtcgatcggt acctccggtg gaggcggcgc
     1021 ccccgcggag tgaccgacgc tggatgccgt gagcgacttg accatgcccc tgaccgtccg
     1081 cactgcccgt cgtcgcacga ccgcaaccgc acggcgcacc gggctgatca cgatcgtcac
     1141 cgggggcggg ccgatcggcg ggcacgacca ccgctgcgcg agcaccgcca tggcggaggc
     1201 cctgccggac gcgtgcggcg tcgtcgaatg ccgggtcgtc gaagccaccg ccgggacccg
     1261 gctcgccccc ggactcgccg gcgccgggct ggtcgtgccg tcggtgcccg gtgagctcct
     1321 cgctccgctg gccgcgctgt gcgagtggct ggagatcccg tatgccggcg cctcaccccg
     1381 cgcgtcaacc ctcgctgccg acgccgagtt ggctgctctc gcactggatc gcgccggcct
     1441 agatgtcgtc cctggtctcg ctgtcgacgc ggctggggcc gccggcctgg actatctcga
     1501 cccggtgcgt atccgcccgg ccggacgccc cggtcccttc gggcgcagca tcgccaccac
     1561 acgtgcgagc ctgcgcgacg gcctccatct cgccctcgcc ctcgacgaac gggcctatgt
     1621 cgaggagatc ccccaaggga tggccgtcat ggtgcccgtc ctcgacgacg gcaccggcaa
     1681 cctgctgacc ggagcggtcc acgagatcgc gcccggcacc ctcttcgccg ccgatcggcc
     1741 ggctgccccc ggcgacgatt ggcacgatgc ccacttgagc cccacggatg gcgcgttggt
     1801 gcgacgagcc gcgcgggcgg cataccgggc actcgggatg cgcggggtgg tcagcgtcga
     1861 gatcatcctc gacggccgac gcgcgctcat cggcgcgatc gaccccgccc ccgcacaacg
     1921 cccacacggg ctgacggccc ggcagttcgc ggcgagcggc acggcatacc cgcaactcgt
     1981 cgcggccgtc gcccacgctg cccgcggctg aggcgggtca ccggccacgc ctcatactga
     2041 aagacaatca tgactgtgac cactcccggg gcgcgcaccc cgacgccggc cccggcgacg
     2101 atcgaccggg cccgcatcgg tatgccgccg cgctgggtcg cgcgggccct cgccaccgcc
     2161 gcctgcctct acggtctctt cgccgtcgtc accgggcaga tgtacgacaa cacggcgtgg
     2221 gtgctgctct tcgggatcat cggcccggcc gtggctctgc tcggggccga ccgttcaccc
     2281 gggctcgggt tcgtggtgtt cgcgggcagc ctcatccttg cgggctacgg gggcggctgg
     2341 gtgtcgcagg tcgcgatccc gatcggcgtc atcctgctct tcgcgtgcac cgccttcggc
     2401 tcgcgaacca cggtggtgct caccggtatt gccatggccc tcgtgccggt ggtcgggttc
     2461 gtcctctggg cgttcggcaa ctacggcatc tcgtcgatcg acgccttcct ccgccagctg
     2521 tcgtgggccc aggtgccctc cgtcatcgcg cgcgccttcc ggaacttccc cggctgggca
     2581 gccctcgtct acctcgtgat cgtcgtgctg ccgtggggag ccgggctggc ctggcgttcg
     2641 gtggccggct cgcgtcgtgc tgccgtcgcc gccgtcgagg agcgcgacca agcgaaccgg
     2701 gagacggcct acgctcagga gatcgcgcac ctgcgggccg ggcagaccca gcttgcgcgc
     2761 gacgtccacg acgtcgtcgg acacagcctc gccgtcatcc tcgcccaggc ccagtcggcg
     2821 cagtacctcg gcgacgacga agtcgacaag atgcgcgaaa cccttgggca cgtagccgat
     2881 tcggctcgcc gctcactcgg ggacgtgcga ggtgtcttgt ccggcacccg cgaggccacc
     2941 agcgccgcga gcgcaggtct cgactccctc atcgccggag tgcgcaacgc gggcaacgag
     3001 gtcgtcgaac gcgtcgacgg gactccccgg ccgctgcctc cggagctgga gcaggtcgcc
     3061 taccgggtcg tgcaggagat gctcaccaat gcgctcaagc acggccggga gggaggcccg
     3121 gtttgggtgc gtcaggtctg gcccgcgcag ccggaggggc atctgctgct cgaggtgcac
     3181 aacctcatgg acaccggccg gacggcatcg ggccacggca tcgggctggg atcgatgcgg
     3241 gaccggctcg cctcggtggg cggcagcctg cacgtggaga ccgaggacac cgtcagcggg
     3301 ccgatgtatg ccgcccgcgc cgacttgccg ctgcgcgccg ccgagatcgc cgaagaaggg
     3361 tgaacgtgaa cgatattcga gttctcctcg tcgacgacca ggagctcttt cgcgccggaa
     3421 tcgcccgcat cctcgaggcc caggagggca tcacggtcgc cggggaggcc ggggacggtg
     3481 cggaggcggt ggcccgggtc gacgagatcg cgccggacgt ggtgctcatg gacatccgta
     3541 tgccggtcat ggacggcgtc gaggccaccc ggcagatctt tgctcctgcg cgagttgcca
     3601 agcgggagaa gccggttcga gtcgtcgtcc tgacgacgtt caacctcgac gatcgggcgg
     3661 cgacggcgat ccgctacggc gccagtgggt ttctcctcaa ggacgcgacg ccggtgatgc
     3721 tgcgcgaggc catccacacg gtgtatgccg gcaacgccgt cctcgccccg tccgatctga
     3781 gtaccttgct ggacgggcag ttccgggcgc gcaagccatt gccggccgac ttccactcac
     3841 tgacggaaaa ggagcgcgag gtgctggctt tcgtggctcg cggcctgtca aacgccgaga
     3901 tcgccgccgc gatgttcgcc agcgaatcga ccatcaagac ccatctcggc gcagtcctgc
     3961 gcaagctcgg actgcgcgac cgggtccagg ttgtcgtttt cgcccacgag cacgggctga
     4021 gcgagtgaac tgggcggcac cctgcgccgc cgcgcccgcg ctggtttggt aagttccccg
     4081 ccgtgggaag actgcggcag gccgcgcatg cggccggaca gctgacgaag agtgggctac
     4141 gacgtcccga ctcgccgact gcgcccgctc cgccgatcgc gcgccggtcg gccgccgaga
     4201 cggcggcggc cgggcccgcc cagacccacg acgtggcgag cgcgctcgcc gatgccatgc
     4261 gcatcgacgg cgctctcgca gtggcattgg cagacagccg ctccggtgag gtgctcgcgg
     4321 ccgatggcga tgtcgacggt atgcagttgg ccaccgccgt cgccggcaat gcccgggtgg
     4381 tgcgggccaa gctcgacaca ttgcacgacc tcgggatgga cgagcggatc gaggacatcc
     4441 tcatcaccct cgactcgcag taccacatca tccgaacctt cgcgaagcgc gacggcctgt
     4501 tcctctacct cgtgctcgac aagccgctcg ccaacctggc gatggcccgg ttcaaggtgg
     4561 ccaccttgga acgggacctc gaactttgac cggaagtccg ggtcagctca tggacgaggg
     4621 cagcacgcct gcggactcgt ctgagaacgg tcggctgggc gtcgctcgcg atcggctcca
     4681 cgcggcgcgc accgagactc tcgaacgcct cacggggctg ctcggcgaga tcgccgggat
     4741 cgtcgccgcg tcggagggct cgaacgccga cgacgagcac gacccggagg gcgcgacgat
     4801 cgcctacgac cgcgcccaaa ccgacgccct cgcgcagcag gcgcggcacc agctggccga
     4861 gatcgatgcc gccctggccc gcgtggaaaa cggcacctac ggccggtgca ccgtctgcgg
     4921 tgaacggatc cccgaggcgc gcctggaggc tcggcccacg gcaaccacgc acgtcgaaca
     4981 cgctgcgccg cgacgctgat cctgcccgcc cggcatcacc gcccgctggg tgctaggcgg
     5041 tgcggcgctc cggcgcggcc actcggcgca ccaggtcggg ggtgaccagg aggacttcgg
     5101 tgagcaactc gccgaaaagg gtctcggcat acgtccgcca ggcccgatcc gcagccgtca
     5161 ggaagggcaa gccgggacgc acccgcgcga gcacgatccg gccgtcggcg gcaccgacca
     5221 tcgcggcgaa ggtgaccagg ccggccggca gcgcgtcctg cagcacctcg tcgccgacgt
     5281 cgtcgatcat gatcggctgg accaccccgc cttcgtcgtc gagccacagg atcgccagcg
     5341 cgggcacgcc ccgcagctgc ggcggcacca tctgatcgat gaggcgcgcc gcgtcggtgt
     5401 cctcaccagg gcgttggcgc gcgtgcaggg ggctcgggat ttcttctcgt tcgctcatgc
     5461 tcaccaagcc tgacagcagt gctcgccggc cgggctgagt tatccacacc caccctgctg
     5521 gcctcctccg ggccggagta gattgggatg tagccgaggt caacgttgcc tgcggcgacc
     5581 gcctggctgt gcgacggtga cccggtcctt cggacaggag cgtgccatga aggcactggt
     5641 tttccatggt cctggcgagc gggcgtggga ggaggtcgca gatcctgcca tcgtcgagcc
     5701 caccgacgca ttggtgcgga tcgacacgac cacgatctgc ggaaccgacc tgcacatcct
     5761 caagggtgac gttccggcgg tgacccctgg ccgcgtcctc ggccacgagg gcgtgggcac
     5821 agtcctcgag accggcaccg gatgcacgag ggtccgggcc ggacagcggg tgatcgtgtc
     5881 gtgcatctgc aagtgcggca cgtgcggcta ctgcaagcag ggtatgccgt cgcactgcca
     5941 gacgctcggc gggatcggct gggtcttcgg tcacctgatc gacggcacgc aggcagagta
     6001 cgtgcgagtt cccttcgcgg acaacggttt agtacccgtg cctccgggcg tgagcgacga
     6061 gcaggccgtc atgctgtccg acatcctgcc gacgggttat gagatcgggg tgcagtacgg
     6121 cgcggtcggc gcgggcgatg tcgtcgccgt cgtcggtgcc ggcccggtcg gcctcgcggc
     6181 catcatgact gctcgcccgt tgggggcatc gcggatcatc gccgtcgacg gtgatccctt
     6241 ccggctggag gcggcgtgcg cgttcggcgc caccgatgtc atcgacatcg gcgcagtgga
     6301 cgatgtcgcg gcggcgctgc ggaggatgag ccccgacggc ctcggggtcg acgtcgcgat
     6361 cgaagccgtc ggcgtccccg ctaccttcac cacctgcctc gacgccatcc gtcccggcgg
     6421 cgtggtggcc aatgtcggcg tgcacggcaa gccggtggag ttcccgatcc agcgggcctg
     6481 gatcgacaac atcaccattc ggaccggcct ggtgaacgcg accacgaccg aggacctcct
     6541 gggcctgatc gcggcccacg agatcgcccc ggagcgcttc gccacccatc acttcaagct
     6601 ggcccagatc atggacgcct acgacgtctt cgccgccgcc gccgaccacc acgccctcaa
     6661 ggtcgtcctg acagcgtgat tctcggacga tcaaggcaaa cgcgacggcg agagccgccc
     6721 gcccagcgct gagacgaaga tcaagaagtg atctgcatga cgacggcctt gggctcggtg
     6781 aagaactcac ggctgaagtt gccgccttca cggccgacgc cggagtcgcc gacgccgccg
     6841 aacggggtgc gcaggtcgcg gatgaagaag cagttgaccc acacagtgcc agcccgcagc
     6901 ttcgaggcga cacggtgcgc acgggagagg ttctccgtga acagcatggc gttgagtccg
     6961 tagcgggtgt cgttggcgag gtggacggct tcctcttcgg tctcgaagcg gttgacgaca
     7021 cagaccgggc cgaagatctc ctcgcaatag ggggcggcgt ccagcgcgag gccgtcgatg
     7081 atcgtcggct tgacgaccca ggacccgtcc tcggcgatgc cgccggtgtg gaaggtgccg
     7141 cccaggtcgc gcgcaccctc gacataggag acgaccttgt cgaagtgccg cttgctggcg
     7201 agggaggaga agttcgtcgc cgggtcgaag gggtcgccga tcgtcagggc ttcggctgcg
     7261 tcgacgaacc gcttcatgaa ctcgtcgtag atggcgtcct ggacgaagag ccgggagccc
     7321 gccaggcaga tctggccggc gttgcggaag atcgcctcga cggcccagta gaccgcattg
     7381 tcgatgtcgg catcggcgaa gacgatattg gcacccttgc cgccgagctc caacgagact
     7441 ggcgccagat gcgttgcggc cgaagccatc acggccttgc ccgtggtgct ggagccggtg
     7501 aaggtgaccc ggtcgacgcg gtcgtcgccg gtgatggcgg agccggccgg gtgcccgtag
     7561 ccgttgagga cgttcaggac gccaggcggg aagccggcct cggcggcgag cccgccgaga
     7621 taggtgaccg acgcgggagt gtcctccgac ggtttgatga tgcacgtgtt gccccaggcg
     7681 atggcgggcg cgatcttcca gctggccatc atgaggggga agttccaggg cgagatggcc
     7741 gcgacgacgc cagccggccc gaactcgctg tagctgtggt ggccgctgtc catcgggtaa
     7801 acctcgccga cgctgtcgcg ctggtggtcg gcgaagaacc gaaagttcca gaccgagcgg
     7861 gccacgtcgt gcttggcctg ctcgaaaggc ttggccatat tggtcgagtc caaggtggcg
     7921 atctcgtcgg cgcgggcctc catgaggtcc gccagtctgt ggatcatctt ggcccgctcg
     7981 acccggccca tccggggcca cggcccctcg tcaaaggcct tgcgcgcgct ggtgatcgcg
     8041 cgatcggcgt ccttctcacc gccctcagcg gcctgtgccc agacctcccg cttccacggg
     8101 ttccagacat cgaagcgctt gccgtcgatc gactcgactt cctcgttgtc gacgatgtgt
     8161 ccgaagaact ccagatcagc catgccccta ctctcctctg cccccgcccc gttccgaagc
     8221 acccgtttcc ccatgcggaa cggtgcacgc cgagcgccct gtcaacagtc gaaagcgcac
     8281 gacgcgccgt agtgcttttt cattacggcg cgtcgtgcac gctcgacgag gttcagggcc
     8341 gcgcggcgcg gcgttcggcg atggtgacgt cgccggcggc ccaatgggtc gggggcacct
     8401 cgcggagcac gacccggatc gactcccggg gagcgccgac ggcctcgacg gccgcgtcgg
     8461 tgagcttggt gatcagggtc cgcagctgct ccgggctgcg cccctcggcc agggtgacgt
     8521 cgatgaacgg cataccgcta gcctgcctcg ccaccgttga ggaacaccga cccgaggttg
     8581 gtgaagtggc acgcgatcga cgagcccggc ggcgcgaaga ccgcgtcggt catgccgccg
     8641 gtgaggacga tccagccggc ctcgatggcg tggccgcggc tggccaggtc gttggccgcc
     8701 agcgcgagcg cctcgcccgg gtgtccctgg acggcggccc cggtggcgga gtcgacgatc
     8761 tcgccgccga cctcgacgag gacgccttcg agggcgaggt cgagttcgct cggcgacagc
     8821 ccgaccgggc cggtgacgaa ggcgcccgac gacgcgttgt cggccgcgac gtcgccggcc
     8881 gtgaaccgga agtccttgta gcggctgtcg atgacttccg ctccgcccca gacggattcg
     8941 accgcggcca tggcctgtgc gttggtgacg ccggggcctt cgagccggtc gcccatgatg
     9001 aagacgattt ccggctcgat acgcgggtgg atcagcttgt cctgcggcac cgggtcaccc
     9061 gtgggcagga tcatcgcgtc ggtgagccac gccacgaagg ggaagtgcac ccccatccgt
     9121 tgctgcttgg cgcgactggt caggccgagc ttgacgccga tgagcttctc gccgcgatcc
     9181 agtcgcttct gcaggttgag gtcctggatc tcatagccgg tcgcgaggtc gagttcgggc
     9241 cattcgtcgg tgaagggggg ccgttcgatg cgctcgtcct cgcatcggag cagttctgcc
     9301 gcaatggatt cgacggtcca tccgccctcg ggagccgtgc cgtatgccgg agcgtcggtg
     9361 gtcgtgtccg tcatgtcagc gcacccccgc cgcagccagg tcgccgagcg ccatgcgctc
     9421 cttctcctgc tgcagttcga gggcgatgtc gatgatcatg tcctcctggc cgccgacgta
     9481 gccgtactcg ccgaccttct gcaggatcgc gtgcgcgggc acgtcgtagc gtgctgcggc
     9541 ccgctcggcg tgcagcagga agctggagta gacgccggca tacccctggg tgatcgaggc
     9601 tcggtccatc cacggcagcc gcggaatgaa cggccgcaca acgtcttccg cggccgagag
     9661 cacctcaccg aggtcgacac cggtcttgat gcccatccgc tcgaaggtcg cggcgaggac
     9721 ctcggtcggg ctgttgcccg cgccggctcc gagggcgcac agggcgccgt cgatctgctt
     9781 ggcgccggcc tgataggcca gcacggagtt ggcgatgccg aggctgaggt tctggtggcc
     9841 gtggaagccg acctgggcac catccgggcc gatctcgtcg atcacggcct ggacccgctc
     9901 ctgggcgtcg gagagcacca gagccccggc ggagtcgacg acatagacgc actgggcgcc
     9961 ggcgtcgacc atgatccggg cctgctcggc gagcttggcc gggctcgcct tgtgcgagag
    10021 catgaggaag ccgacggtct ccatgccgag cttgcgggcc tcgccgaagt gctggatcga
    10081 gacgtcggcc tcggtgcagt gggtggcgat gcgggcgacc gaggcaccca tctcgtgggc
    10141 cttcttcagg tggtgcacgg tgccgagccc ggggagcatg aggatggcga tcttggcctg
    10201 cgtggcctct tcgacggcgg ccttgatgag gtcgaattcg tcgaccttgg agaagccgta
    10261 gttgaaactg ctgccgccga ggccgtcgcc gtggctgacc tcgatcacct ggaccccggc
    10321 ccggtcgagg gcgctgacga cgccgcgcac ctgctcctcg gtgtactggt gggccatggc
    10381 gtgcgagccg tcgcgcagcg tggagtcggt gatgcgcaga tcggtctccc cgccggcctt
    10441 ggggaggttc tccaggacct cactctcggt gacgatgtgg tcgaccttcg gggcatcctc
    10501 gggacggatt gtcatgtcgc ccatggtgtt cagactcctg ccttctcggt ctcgacgatg
    10561 gcgtcggggg cgtccccgag atcggccgag gacttccacg acgtttcgac gcccagtcgg
    10621 tgggcgacca tgatgtcgcc gacgcgggct gcggcggcgg tgatgatgtc gaggttgccg
    10681 gcgtagtcgg gcagataatc gccgcggccc ttgacctcga tggggacgaa gacatcatca
    10741 ccgccgccgc agcccgcgtc ggcgacatca
//
LOCUS       DAOPJG010000115        10635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6536_length_10635_cov_7.783365, whole genome shotgun sequence.
ACCESSION   DAOPJG010000115 DAOPJG010000000
VERSION     DAOPJG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10635
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6536_length_10635_cov_7.783365"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="nadE"
                     /locus_tag="PKU75_10885"
     CDS             <1..698
                     /gene="nadE"
                     /locus_tag="PKU75_10885"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777210.1"
                     /note="catalyzes the formation of nicotinamide adenine
                     dinucleotide (NAD) from nicotinic acid adenine
                     dinucleotide (NAAD) using either ammonia or glutamine as
                     the amide donor and ATP; ammonia-utilizing enzymes include
                     the ones from Bacillus and Escherichia coli while
                     glutamine-utilizing enzymes include the Mycobacterial one;
                     forms homodimers; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPF81429.1"
                     /translation="EVYDVTFENVQAGLRTDYLFRLANHNGGIVLGTGDLSELALGWC
                     TYGVGDQMSHYGVNSGVPKTLMQHLIRWVATSGQFDDHVGEILQSILDTEISPELVPQ
                     KEGEKPQSTEDAIGPYSLQDFTLFHVLRRGYLPSKIAFLATHAWTDATVGEWPPNYPE
                     AKRHGYDLATIRHWMAVFGKRYFANQFKRSALPNGPKVIRGGSLSPRGDWRMPSDVSS
                     AVWLADLERVPES"
     gene            complement(702..1127)
                     /locus_tag="PKU75_10890"
     CDS             complement(702..1127)
                     /locus_tag="PKU75_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPF81430.1"
                     /translation="MLDESPVTNLTDEQCWELLEANEFGRLAFHMAGEVHITPINYVV
                     DQGRLIFRTAEGNKLLGMTMNHDVAFEIDQFTEDEAVSVVLRGSADTLEGDAAEFVDT
                     LPLRPWVATTKLVVVAITPEEMSGRRFTLQRPWLHSRKD"
     gene            complement(1260..1922)
                     /locus_tag="PKU75_10895"
     CDS             complement(1260..1922)
                     /locus_tag="PKU75_10895"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HPF81431.1"
                     /translation="MQRFLAGMATMLAIVVAVFVVLFGMLRDPGTQVPHDVSLGPGVR
                     PSQPPADLGPDETWLGDVDLSSSALVTPQGPLRAVEATGTNVRVTRDGLRVGEVRLVA
                     TLPFDVAEEQVGQGVQLYDAGGNRVGVRRSATLLGVDVAVSATGTVRAVGGDLLITPE
                     SVDIGLPGLVNDALSNAARELVTIRQSIQGLPAGLHLTDVTVRADGFRATLRGTDVTI
                     AG"
     gene            complement(1956..3233)
                     /locus_tag="PKU75_10900"
     CDS             complement(1956..3233)
                     /locus_tag="PKU75_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPF81432.1"
                     /translation="MSTGDRTYDLVLFGASGFVGKLTAGHLAQHAPDGARIALAGRTE
                     SKLQAVRDSLGPAAEGWDLLVADAADEPRLAELAASTKVVVTTVGPYAKYGMPLVDAC
                     ARAGTHYADLTGEVLFVRRSADANHELAADTGAKIVHACGFDSIPSDLGVYLTAEQAK
                     ADGAGELTQTTLYVRQLKGGFSGGTIDSMRTQAIETRADAGDRKIVADPYGLSPDRGA
                     EPSRSGPRPPKATNPLSVAGGILKNLPVRRTADGHWLGPFVMASFNTRIVRRSNALLG
                     HAYGTGFRYQEVMDFGTSAKAPVLAAGMTAGLLGVATGMQFGPGRAVLDRVLPKPGEG
                     PSPEAQEKGRFRMVIDARTTSGARYETTVAAQADPGYSGTAVMLGQAGLCLAFDELPD
                     RAGVLTPAVAMADALTARLRAQDFTLETRRVEA"
     gene            3253..4506
                     /locus_tag="PKU75_10905"
     CDS             3253..4506
                     /locus_tag="PKU75_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPF81433.1"
                     /translation="MPRHHVLPSSRTARILAVVQAGGEGSRMDVLTRERAKPALPFAG
                     SYQLIDFALSNLANSQIPHVWVSVQYQAGSLDRHLAQGRPWDLDRTRGGYRRLVPEAG
                     HTDSVAGFSAGNADDLLRMHNDIAELDPDVVIVTSADHIFRLDLREVLARHLDRRAEC
                     TIVTTEVPLAEAKHKAVVRVGRGQLVKGLDYKPSRPASGLVATEVFVYDAPRLLAELD
                     ALRRDRAAVDPGLTEGLGDFGEHLVPRLIARGRTYAHALEGYWKDLGRPAAYLAAHRD
                     LLRGKVDVFDDPRWPMLTRFPELPPAWVAAEGTVADSLLSPGAAVHGTVCGSVVGPGC
                     VIEAGARVVDSVLMAGVRVAAGARVAGSVLDDGVVVEADAVVGAAPRRWPPADTAVTL
                     CGRQSRVRAGVVLPAGGRLEPGGTA"
     assembly_gap    4831..4930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4931..5221)
                     /locus_tag="PKU75_10910"
     CDS             complement(<4931..5221)
                     /locus_tag="PKU75_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81434.1"
                     /translation="MRSEPAGNVLGQVAVGCRRGHTAVIGDLGHPGSAVGATVRATIT
                     GRAVARGAVARGAVTGRTVARGAVTGRTVTSRAVAGRAVTSAAVGTVVRAAVA"
     gene            5279..6535
                     /locus_tag="PKU75_10915"
     CDS             5279..6535
                     /locus_tag="PKU75_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPF81435.1"
                     /translation="MNPTDTSSGADQQVRPALARLLSVSFDDFADKHWGTAPLLSRAD
                     DLPSGFADLFSNDAVDELISRRGLRTPFLRVAKDGSTLADAEFTRAGGVGAGVTDQLS
                     DDRLLDLFAGGSTLVLQALHRTWPPIIDFTGQLSTDLGHPVQVNSYTTPAQNTGFSSH
                     YDVHDVFVLQIAGAKRWQIREPILTTPLRSQPWDQRRAQVEAASTGIPLLEVTLEPGD
                     CLYLPRGYLHAATALGEISTHLTIGVHTWTRTHLLHTLSRYAVTRLADDADLRAALPP
                     GLDWSGVPVAEADLELVRDRLVAALRQVRLSDLADALHTAHRGSQRAEPVAPVAGVAA
                     AGALTSADTLRLRGGLAPHLTETAAGADLLSRAGRRSITATDLPLVRRLLTEGRLSVA
                     DAGEDLARRLIVAGLVVIEPRQPPAG"
     gene            6546..7469
                     /locus_tag="PKU75_10920"
     CDS             6546..7469
                     /locus_tag="PKU75_10920"
                     /inference="COORDINATES: protein motif:HMM:NF018677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HPF81436.1"
                     /translation="MSLPETACSVVARAHDDPLIATAPPTRRWLLLDVPRAWPEKILR
                     SPEVAGAVPALGDIVDRRQGRVLFVRRPGGAVGARLHPDDHGPIRGFLVVDAPTRTQT
                     RGAWRPAAAGRPAADLTAALAAFDADGPLAEPAAEILLVCSHGTHDQCCAVRGRPVAA
                     VLAERYEHVLECSHVGGDRFGGNLLALPTGTYLGRLDADSAPAVVAAHLAGRDDPAHL
                     RGVTTQDQRVQAAVTATLAAYGAAAAYRLHGEIVGHPAPDRWHVSVSGHPAAAQVRVE
                     VRRIVLSPAPRACRAEIDKVAVTYETALLDS"
     gene            complement(7540..7977)
                     /locus_tag="PKU75_10925"
     CDS             complement(7540..7977)
                     /locus_tag="PKU75_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81437.1"
                     /translation="MAEYTEAEHETIRSAAFGAIALVSKADPGFFSTFKESMAGSRAF
                     AAAPPAVQELLKTGGFPTPPKGDAAEVERQILDGLRQSMTILNGKDAAAAGGFRDVIV
                     AAADAVADASKGTSAEEAAVIAKIREAVGGTAGPAAGGTPSLV"
     gene            8041..8781
                     /locus_tag="PKU75_10930"
     CDS             8041..8781
                     /locus_tag="PKU75_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPF81438.1"
                     /translation="MTHPHASSPVPNATSTASSQVHGLDRLEGRRFAAVLFDLDGTLI
                     DSTAAIARCWAAWAPTYGIELSRLRGLHGVPARGIIERLLTEERRDEAEQHIRRLELD
                     DVSEIPTLPGAVAALEALAGARHAIATSCTDDLAQARIGATGLPRPPVVVTASDVARG
                     KPAPDPYLLAAERLGADPRDCLVVEDAPLGLESARAAGCATLAVTTTTAYDQLVADPN
                     ADAVIPDLAAVHFSVTGDGRVRLAPGRP"
     gene            complement(8785..9390)
                     /locus_tag="PKU75_10935"
     CDS             complement(8785..9390)
                     /locus_tag="PKU75_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HPF81439.1"
                     /translation="MSEQQTAGRPGEAPAEQQNPDRVVVLTPEGMGVHDPSDEERNPS
                     ELVEQPAKVMRVASMIKQLLEEVRSSPLDDAGRARLAQIHQRSIAELKDGLAPELVQE
                     LDRLALPVGEGSTPSDAELRIAQAQLVGWLEGLFHGIQAALVAQQMAAQAQLQQMRRE
                     LPPGHPAAVPEQQPMPGPAARPGNPPFVVPSDGVGGTGQYL"
     gene            complement(9436..10326)
                     /locus_tag="PKU75_10940"
     CDS             complement(9436..10326)
                     /locus_tag="PKU75_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPF81440.1"
                     /translation="MTAVTSTSASTPIAANRATRGPGWIRRAIAQLFVGLILVLLVLA
                     VIYPLLWMVFSSFKNNSELFANPWSLPGELRWGNFERAWNAGVVRFFVNSTLVTAASI
                     VTTTLISAWAAYGLVRVNVPFSGPITGLLLGGLMVAPTVALIPLFGLLQRLHVYDSVW
                     ALLILYTAFRIPFTTFLIRAYLIDMPREMDEAAYVDGATRTQIFWRLVLPVCRPILVS
                     AALLQALFAWNEFAFALVFISNTENKTLPVGLMDMQSRLLTEWPVQFAGLTMAALPMI
                     ILFLIGQRQFLRGLTEGMGK"
     gene            complement(10328..>10635)
                     /locus_tag="PKU75_10945"
     CDS             complement(10328..>10635)
                     /locus_tag="PKU75_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF81441.1"
                     /translation="VDGAGAVRSFFSVTIPMVREMTTLLIILTVSGAFLVFNEVMVMT
                     AGGPSDSSQVLGTWLYRNAFLADNMGYASAIATVIFVLTFAFAILQILVSRRRRVEI"
BASE COUNT         1510 a         3751 c         3796 g         1478 t
ORIGIN      
        1 aggaggtcta cgacgtgacc ttcgagaacg tccaggccgg cctgcgcacc gactacctgt
       61 tccggctcgc caatcacaac ggcggcatcg tgctcggcac cggcgacctg tcggagctgg
      121 cgctcggctg gtgcacctac ggggtgggtg accagatgtc gcactacggc gtcaactccg
      181 gggtgcccaa gacgctgatg cagcacctga tccggtgggt cgcgacgtcg ggtcagttcg
      241 acgaccacgt cggggagatc ctgcagtcga tcctcgacac cgagatctca cccgagctgg
      301 ttccgcagaa ggagggcgag aaaccccagt cgacggagga cgccatcggg ccctactccc
      361 tgcaggattt caccctcttc cacgtgctgc ggcgcggcta cctgcccagc aagatcgcct
      421 ttctcgccac acacgcctgg accgacgcca ccgtgggcga gtggccaccg aactatcccg
      481 aggccaagcg gcacggctac gacctggcga ccatccggca ctggatggca gtcttcggca
      541 agcgctactt cgccaaccag ttcaagcgct ccgcgctgcc caacgggccc aaggtgatcc
      601 ggggcggttc gctgtccccg cgtggcgact ggcggatgcc gtcggacgtc tcctcggccg
      661 tctggctcgc ggacctggag cgggtgccgg agagctagcc gtcagtcctt gcgggagtgc
      721 agccagggcc gctgcaacgt gaagcggcgc ccgctcatct cctcgggggt gatcgccacg
      781 acgaccagct tggtggtggc gacccagggc cgcaggggga gggtgtcgac gaactcggcc
      841 gcatcgccct cgagggtgtc ggcgctgccg cgcaggacga cgctgacggc ctcgtcctcg
      901 gtgaactggt cgatctcgaa cgcgacatcg tggttcatcg tcatgccgag cagcttgttg
      961 ccctcggcgg tgcggaagat cagccgtccc tggtcgacga catagttgat cggcgtgatg
     1021 tggacctcgc cggccatgtg gaaggccagg cgcccgaatt cgttggcctc caacagctcc
     1081 caacactgct catcggtcag gttcgtcacg gggctttcgt cgagcatgac gtgtctcctt
     1141 cgatcccagg ggagccggcc cgcagccgga ctgtcgctga tgccccaacg ctaatacgcc
     1201 cggggaagac gcggtgcgct ggtccggcaa cgacgtgatc acactgcccc ggtgcccgcc
     1261 tagccggcga tggtcacatc cgtgccgcgc agggtggcgc ggaagccgtc ggcgcggacc
     1321 gtcacgtccg tgaggtgcag ccccgccggg aggccctgga tgctctggcg gatcgtcacc
     1381 agctcgcgcg cggcattgct caacgcgtcg ttgaccaggc ccggcaggcc gatgtcgacg
     1441 ctctcggggg tgatgagcag gtcgccgccg accgcccgga ccgtccccgt cgcgctcacc
     1501 gcgacatcca cccccagcag cgtcgccgac cggcgcacac ccacccggtt gccgccggcg
     1561 tcatacagct gcacgccctg gcccacctgc tcctcggcca cgtcgaaggg caaggtggcc
     1621 accagccgca cctcgccgac gcgcaggccg tcgcgggtga cgcgcacgtt ggtgcccgtc
     1681 gcctcgaccg cccgcagggg tccttgcggg gtcaccagcg cgctggagct gaggtcgacg
     1741 tcgccgagcc acgtctcgtc cgggccgaga tcggcggggg gctggctggg gcgcacgccc
     1801 gggccgagcg agacgtcgtg cgggacctgg gtgccggggt cgcgcagcat gccgaagagg
     1861 acgacgaaga ccgcgacgac gatggcgagc atcgtcgcca tccccgcgag gaatcgctgc
     1921 acgccggccg ccccggtggt atgccgcccg ctcgcctacg cttcgacccg ccgggtctcg
     1981 agggtgaagt cctgggcgcg cagccgggcg gtgagggcgt cggccatcgc cacggctggg
     2041 gtgagcaccc cggcgcggtc gggaagttcg tcgaaggcga ggcagaggcc cgcctggccg
     2101 agcatgacgg ccgtgccgct gtaaccaggg tcggcctgcg ccgcgacggt cgtctcgtag
     2161 cgcgccccgc tcgtcgtccg ggcgtcgatg accatgcgga agcggccctt ctcctgcgcc
     2221 tccgggctcg gtccctcgcc cggcttgggc aggacgcggt cgagcacggc tcggccggga
     2281 ccgaactgca tacccgtggc gaccccgagg aggccggccg tcatcccggc tgccaggacc
     2341 ggcgccttgg cggaggtgcc gaagtccatc acctcctgat agcggaaccc cgtgccatag
     2401 gcgtgcccga gcaacgcatt ggagcggcgc acgatgcgcg tgttgaacga ggccatgacg
     2461 aacggcccga gccagtgccc gtcggcagtg cggcggacgg gcaggttctt gagtatgccg
     2521 ccggccaccg acaacggatt ggtcgccttc ggcgggcggg ggccgctgcg ggacggttcg
     2581 gcgccgcggt cggggctgag gccgtaagga tcggcgacga tcttgcggtc gccggcgtcc
     2641 gcgcgcgtct cgatcgcctg ggtgcgcatc gagtcgatgg tgccgccgct gaagccgccc
     2701 ttgagctggc ggacatagag ggtggtctgc gtcagctcgc cggcaccgtc ggccttggcc
     2761 tgctcggccg tgagatagac gccgaggtcg gaggggatcg agtcgaagcc gcatgcgtgc
     2821 acgatcttgg ctccggtgtc ggcagcgagc tcgtgattgg cgtcggcgct gcggcgcacg
     2881 aagaggacct caccggtcag gtcggcgtag tgggtgccgg cgcgagcgca agcgtcgacg
     2941 agcggcatac cgtatttggc gtaagggccg acggtggtca cgaccacctt cgtgctcgcg
     3001 gcgagttcgg cgaggcgggg ctcgtcggcg gcgtcggcga cgagcaggtc ccagccctcc
     3061 gcggcggggc cgaggctgtc gcggacggcc tggagcttgg actcggtgcg cccggcgagg
     3121 gcgatgcggg cgccgtcggg ggcgtgctgg gcgaggtggc cggcggtgag tttgccgacg
     3181 aagccgctcg cgccgaagag gacgaggtca taggtgcggt cgccggtgct catgtcgggc
     3241 agtctaggtc gcatgcctcg gcaccacgtc ctcccgtcgt cgcgcactgc gcgcatcctc
     3301 gcggtcgtgc aggcgggcgg tgagggctcc cgcatggatg tcctgacgcg ggagcgggcc
     3361 aagccggcgc tgccgttcgc cggcagctac cagctcatcg acttcgccct atcgaatctc
     3421 gccaacagcc agatcccgca cgtgtgggtg tcggtgcaat atcaggcggg ttcgctggac
     3481 cggcatctcg cgcagggtcg cccgtgggac ctcgatcgca cccgcggcgg ctatcggcgg
     3541 ctggtgccgg aggcgggcca cacggactcg gtagccggat tcagcgccgg caatgccgac
     3601 gatctgctgc ggatgcacaa cgacatcgcc gaactggatc cggacgtcgt catcgtcacc
     3661 agcgcggacc acatttttcg tctcgacctg cgggaggtgc tcgcgcggca cctggaccgc
     3721 cgggccgagt gcacgatcgt gaccaccgag gtgccgttgg cggaggccaa gcacaaggcg
     3781 gtggtgcggg tcggccgcgg ccagctcgtg aagggattgg actacaaacc ctcgcgtcct
     3841 gcctcgggcc tcgtcgcgac cgaggtgttc gtgtatgacg cgccgcgcct cctcgccgaa
     3901 ctcgacgccc tgcgccggga ccgggccgcg gtggacccgg ggctgacgga ggggctgggc
     3961 gatttcgggg agcacctggt gccccggctg atcgcgcggg gccggacgta tgcccatgcg
     4021 ctggaaggct attggaagga cctcggccgc cccgcggcat accttgcggc gcaccgggac
     4081 ctgctgcgcg gcaaggtgga cgtattcgac gacccgcgct ggccgatgct cacccgcttc
     4141 cccgaactgc cgcccgcgtg ggtggcggcg gaggggacgg tcgccgactc gctcctctcg
     4201 ccgggcgcgg cggtgcacgg cacggtatgc ggcagcgtcg tcggccccgg atgcgtcatc
     4261 gaggcgggtg cgcgagtcgt ggacagcgtg ctgatggccg gcgtccgggt cgccgccggc
     4321 gcgcgggtgg cggggtcggt cctggacgac ggcgtggtgg tggaggcgga cgcggtcgtc
     4381 ggggccgcac cgcgccgctg gcccccggcg gacacggccg tcaccctctg cggccggcag
     4441 tcccgcgttc gggcgggggt ggtgcttccc gccggcggcc gtctcgaacc cggcggcacg
     4501 gcctgacccc tccctcggtg acatctcgac ggggtcgaat cgacatctcg acgtcgtgag
     4561 tgccgcggcg ggtgaggacc gttgggcgca tcggataaat tgtcgggtcc taacggcgcg
     4621 cagcgcgcgt gattgcggtt acatgacagg cacgggcgac cggcccggat gtgcgagcta
     4681 ctgaacggaa ggatccaagg atgagcattc cccctggcga aggcaccgag gcccccatcg
     4741 agaccatgga ccaagagtcg cccgcggtgg ccggtgacgg cggcgcggac cacggcgccg
     4801 acggtggcgc tggtgacggt gctgctggcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn ggcgacggcg gcgcggacca cggtgccgac ggcggcgctg gtgacggcgc
     4981 ggccggcgac ggcgcggctg gtgacggtgc ggccggtgac ggcgccgcgg gcgacggtgc
     5041 ggccggtgac ggcgccgcgg gcgacggcgc cgcgggcgac ggcgcggccg gtgatggtgg
     5101 cgcggacggt ggcgccgacg gcggagccgg gatgaccgag gtcgccgatg acggcggtgt
     5161 ggccccggcg gcacccgacg gcgacctgcc cgaggacgtt gcccgcgggc tctgaccgca
     5221 tgagtggtga tgcgtctgac gcacatcatc acgcgggagg accggacata atctgcgcgt
     5281 gaacccaacc gacacatcga gcggggcgga ccagcaggtc cgccccgctc tcgcgcgcct
     5341 gctctccgtt tccttcgatg atttcgccga taagcattgg ggcacagcgc ctttgctctc
     5401 ccgggcggat gacctgccga gcggcttcgc cgacctgttc agcaatgacg ccgtcgacga
     5461 gctcatctcc cggcgcggcc tgcgcacccc gttcctgcgc gtggccaagg acggctcgac
     5521 gctggccgac gcggagttca cccgggccgg gggcgtgggc gccggcgtca ccgaccagct
     5581 cagcgacgac cggctcctcg acctcttcgc cggcggctcg acgctggtcc tgcaggcgct
     5641 gcaccgcacc tggccgccga tcatcgattt caccgggcag ctgagcaccg atctgggcca
     5701 cccggtccag gtcaactcct acaccacccc cgcccagaac accgggttca gctcccacta
     5761 cgacgtccac gacgtcttcg tcctgcagat cgccggcgcc aaacgctggc agatccgcga
     5821 accgatcctg acgaccccgc tgcgcagcca gccctgggat cagcggcgag cgcaggtcga
     5881 ggcggccagc accggcatac cgctgctcga ggtcaccctc gaacccggcg actgcctcta
     5941 tctcccgcgc ggctacctgc acgccgccac cgccctgggc gagatcagca cccacctgac
     6001 catcggggtg cacacgtgga cgcgcaccca cctgctgcac accctcagcc ggtatgccgt
     6061 gacgcgcctc gccgacgacg ccgacctgcg cgctgccctc ccgcccggtc tcgactggtc
     6121 gggcgtcccg gtcgccgagg cggacctgga gttggtgcgc gaccgtctcg tcgccgcgct
     6181 gcgtcaggtc cggctgagcg acctggcgga cgcactgcat acggcgcacc ggggctcgca
     6241 gcgcgccgag ccggtcgccc ccgtcgccgg cgtcgccgcc gccggagccc tcacctccgc
     6301 cgacacactg cgcttgcgcg gcggcctcgc gccgcacctc accgagaccg ccgcgggcgc
     6361 cgacctgctc tcgcgcgccg gccggcgcag catcaccgcc accgacctgc ccctggtgcg
     6421 gcgcctgctg accgagggcc ggctgagcgt cgccgacgcc ggcgaagacc tggcccgccg
     6481 gctgatcgtc gccggcctcg tcgtcatcga gccacggcag cccccggcgg gctagcgttg
     6541 cggagatgtc gctacccgaa accgcctgct cggtggtggc gcgcgcccac gacgacccgc
     6601 tgatcgccac cgcgccaccc acccgccggt ggctgctgct cgatgtcccc cgggcctggc
     6661 cggagaagat cctgcgcagc cccgaggtcg cgggcgcggt ccccgcgctg ggcgacatcg
     6721 tggaccggcg ccagggccgg gtcctgttcg tgcggcgccc gggcggcgcc gtcggggcgc
     6781 ggctgcaccc ggacgaccac gggcccatcc gcgggttcct cgtcgtcgac gcccccaccc
     6841 gcacccagac gcgcggcgcc tggcgaccgg ccgccgcggg acgccccgcc gcggatctga
     6901 ccgccgccct ggccgccttc gacgccgacg ggccgctggc cgaaccggcc gccgagatcc
     6961 tcctcgtgtg ctcccacggc acccacgacc agtgctgcgc ggtgcgcggc cggccggtcg
     7021 cggccgttct cgccgaacgc tacgagcacg tcctcgagtg cagtcacgtc ggcggtgacc
     7081 gcttcggcgg caacctgctg gccctcccga ccgggaccta cctcggccgg ctcgacgccg
     7141 actcggctcc ggccgtggtg gccgcccacc tcgccggacg cgacgatccg gcgcacctgc
     7201 gcggggtgac gacccaggat cagcgcgtcc aggcggcggt gacggcgacc ttggcggcgt
     7261 acggcgcggc ggcggcatac cggctgcacg gagagatcgt cggccacccg gcgccggatc
     7321 gctggcacgt gagcgtgtcc ggtcaccccg ccgccgcgca ggtgcgcgtc gaggtccgcc
     7381 gcatcgtgct gagcccggcg ccccgggcct gccgggccga gatcgacaag gtcgccgtca
     7441 cctacgagac cgcgctgctc gactcctgac gccacgcgag gtgaaacgac cgacggccgg
     7501 cctccccaca ggggacgccg gccgtcagtg cagaggggat cagacgagcg agggagtgcc
     7561 gcccgccgcg gggcccgcgg tgccgccgac ggcctcgcgg atcttggcga tgaccgccgc
     7621 ttcctcggca gaggtgccct tggaggcgtc ggcgacggcg tcggcggcgg cgacgatgac
     7681 gtcgcggaag ccgcccgcgg ccgccgcgtc cttgccgttc aggatcgtca tcgactggcg
     7741 caggccgtcg aggatctgcc gctcgacctc cgcagcgtca cccttgggag gcgtggggaa
     7801 gccgccggtc ttgaggagtt cctggaccgc cgggggtgcg gcggcgaagg cgcgggagcc
     7861 ggccatcgac tccttgaagg tggagaagaa ccccgggtcc gccttggaga cgagagcgat
     7921 ggcaccaaag gcagcgctgc ggatggtttc gtgttcggcc tcggtgtatt cagccatgag
     7981 gccagtgtgc cctcccaagg ggcaccgcgc gcgtcgcagc gtccgcctaa agttgacggt
     8041 atgacgcacc cccacgcctc ctcgccggtc ccgaacgcga cgagcacggc atcgtcgcag
     8101 gtgcacggtc tggaccggtt ggaggggcga agattcgctg ccgtcctctt cgatctggac
     8161 ggcacgctca tcgactccac cgccgccatc gcgcgctgct gggccgcgtg ggcgcccacc
     8221 tacgggatcg agctgagccg actgcggggg ctgcacggcg taccggcccg cgggatcatc
     8281 gagcggctgt tgaccgaaga gcggcgcgac gaggccgagc agcacattcg ccgactcgaa
     8341 ctggacgatg tcagcgagat ccccaccctg cccggtgctg tcgcggcgct cgaggcgctc
     8401 gccggcgccc ggcacgcgat cgcgacctcc tgcaccgacg acctcgcgca ggcgcgcatc
     8461 ggggccaccg gcctcccgcg cccgcccgtc gtcgtcacgg cctccgatgt cgcccgcggc
     8521 aagcccgcgc ccgaccctta tctgctggcc gccgagcgcc tcggcgccga cccgcgcgac
     8581 tgcctggtcg tcgaggacgc gcccctcggc ctggagtccg cgcgcgcggc ggggtgcgcc
     8641 accctcgcgg tgaccacgac cacggcatac gaccagctcg tcgccgaccc gaacgccgat
     8701 gccgtcatcc ccgacctggc cgctgtccac ttcagcgtga ccggcgatgg ccgggtgcgg
     8761 ctggcgcccg gccgcccctg acttctacag atactgtccg gtgccgccca cgccgtccga
     8821 ggggacgacg aacggcggat tgcccggccg ggccgccggc ccgggcatcg gctgctgctc
     8881 cgggacggcg gcggggtgcc ccgggggcag ttctcgacgc atctgctgca gctgcgcctg
     8941 ggcggccatc tgctgggcga ccaaggcggc ctgtatgccg tggaagagcc cctcgagcca
     9001 gcccaccaat tgcgcctggg cgatccgcag ctcggcatcg ctcggggtgc tgccctcacc
     9061 gacgggcagg gcaagccggt cgagctcctg gacgagttcg ggggcgaggc cgtccttgag
     9121 ctcggcgatc gagcgctggt ggatctgggc cagccgggcc cggccggcat cgtccagcgg
     9181 cgaggagcgc acctcctcga gcaactgctt gatcatcgag gccacccgca tcaccttcgc
     9241 cggctgctcg acgagctcgg acggattgcg ctcctcgtcg ctcgggtcgt gtacccccat
     9301 cccctcgggg gtcaggacga cgacccgatc cgggttctgc tgctcggctg gggcctcgcc
     9361 gggccggccg gctgtctgct gctcgctcat gacgccatct tgccgcaccc cccccgacgt
     9421 ccgagccgtg gcggcctatt tgcccatgcc ctccgtcagg ccgcgcagga attgccgctg
     9481 cccgatcagg aacaagatga tcatcggcag cgccgccatc gtcaggcccg cgaactgcac
     9541 cggccactcg gtgagcaggc gcgactgcat gtccatcaag ccgaccggga gggtcttgtt
     9601 ctcagtgttg gagatgaaga ccagcgcgaa cgcgaactcg ttccacgcga aaagcgcctg
     9661 cagcagggcc gcggagacca ggatcgggcg gcatacgggc agcaccagtc gccagaagat
     9721 ctgcgtgcgg gtcgccccgt cgacataggc cgcctcatcc atctccctcg gcatgtcgat
     9781 cagataagcc ctgatcaaga aggtggtgaa cgggatccgg aaggcggtat agaggatgag
     9841 cagcgcccac acggagtcat agacgtgcaa ccgctgcagc aggccgaaca gcgggatcaa
     9901 ggcgacggtc ggggcgacca tcagcccccc gagcagcagc cccgtaatag ggcccgagaa
     9961 gggcacgttc acccggacca ggccgtatgc cgcccacgcc gagatcaacg tggtcgtcac
    10021 gatggaggcg gcggtcacca aggtgctgtt gacgaagaag cggacgacgc cggcgttcca
    10081 ggcccgctcg aagttgcccc atcgcagctc accgggcagc gaccacgggt tggcgaagag
    10141 ttcggagttg ttcttgaaac tggagaagac catccacagc aggggataga tcaccgccag
    10201 cacgagcagg acgaggatca gtccgacgaa gagctgggcg atggcccggc ggatccagcc
    10261 ggggccgcgg gtggcgcggt tggcggcgat gggggtcgag gcggatgtcg atgtcacggc
    10321 ggtcatgtca gatctccacg cggcggcgcc ggctgaccag gatctgcagg atggcgaagg
    10381 cgaaggtcag cacgaagatg accgtcgcga tcgccgaggc ataacccatg ttgtcggcga
    10441 ggaaggcatt gcggtagagc caggtgccca gcacctgact ggaatcggag ggtccgccgg
    10501 cggtcatgac catgacctcg ttgaagacaa ggaaggcgcc cgagacggtg aggatgatga
    10561 gcagcgtggt catctcgcgc accatcggga tggtcacgga gaagaaggag cgcacggcgc
    10621 cggccccatc aacct
//
LOCUS       DAOPJG010000116        10614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6563_length_10614_cov_8.224264, whole genome shotgun sequence.
ACCESSION   DAOPJG010000116 DAOPJG010000000
VERSION     DAOPJG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6563_length_10614_cov_8.224264"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKU75_10950"
     CDS             complement(<1..638)
                     /locus_tag="PKU75_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPF81442.1"
                     /translation="MTAQRANLTKFAWLSIAAAIATIALKFGAYVLTGSVGLLSDAAE
                     SIVNLVAAFVALLALHVASRPPDDDHHYGHSKAEYFSAVVEGVMIFVAAVFILLTSVE
                     RFLHPQGLQNVGVGLGISVIASVLNGTVAMLLLRAGRAHNSITLVADGKHLLTDVWTS
                     VGVVVGVLLVAVTKWERLDPIIAFLVGCNIIWTGWHLIRQSVDGLMDKTIDA"
     gene            complement(687..1637)
                     /gene="rarD"
                     /locus_tag="PKU75_10955"
     CDS             complement(687..1637)
                     /gene="rarD"
                     /locus_tag="PKU75_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HPF81443.1"
                     /translation="MTPPPAVARSSEADRVRRGTAYGLLAYGIWGIFPIYFHALKPAG
                     AWEILAHRIVWTMLLCLAILAVRRDWAWMRTVARSRRLSLGLIAAALVIAANWVIYVF
                     AVLSGHTYEAALGYFLNPLVTVALGVVILGEGLRPLQWLAVAVGAIAAIYLALEGGHF
                     PWIAISLALSFGTYGLLKKRLGVALDPWHSLFAETAVLTPVAALILILVAGAGDATYG
                     ASPGHTTLLTLAGIITAVPLLLFAAAARRVPLVTIGLIQFMTPVMQLLVGVIVLHEHV
                     SGRLWVGFGIVWIALLLLTIDSLQAARRARGIPPLDPAAA"
     gene            complement(1634..2698)
                     /locus_tag="PKU75_10960"
     CDS             complement(1634..2698)
                     /locus_tag="PKU75_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPF81444.1"
                     /translation="MPLPRGLEGMHRLGGDAKLTKKDYTSDQEVRWCPGCGDYIILNT
                     VQSFLPELGLRKENIVFVSGIGCSSRFPYYLDTYGMHSIHGRAPAIATGLATSRPDLS
                     VWVVTGDGDALSIGGNHLIHALRRNVNMTILLFNNRIYGLTKGQYSPTSEQGKITKST
                     PAGSVDTPFNPVSLALGAEATFVARSLDNDRAHLTEILKAAAAHRGTALVEIYQNCPI
                     FNDGAFDAIKDRDQQQARLVHLAQGQPVTVGAEDDRKVLVREGGKLVFVPEAEAGDRE
                     VIVHDPTDPDPSRQFELSRIDDSSMTHVPMGIFRQVERPTYDDLVRGQIEAAVTTAGA
                     PATDADLAALLAGRDTWTVG"
     gene            2912..3268
                     /locus_tag="PKU75_10965"
     CDS             2912..3268
                     /locus_tag="PKU75_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPF81445.1"
                     /translation="MEAYVVAAAVLGAGVLLFVAAMAARWLFAPRTRTVASGTTYESG
                     VDPVGTGWAQTQVRYLVYAFLYVIFAVDAVYLFPWALVARGHLGRASLVEVGVFIGII
                     LIGLAHAARRGLLKWT"
     gene            3340..3867
                     /locus_tag="PKU75_10970"
     CDS             3340..3867
                     /locus_tag="PKU75_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPF81446.1"
                     /translation="MRVVLNWGRRYSLWVFNFGLACCAIEFIAASMGRHDFMRLGVIP
                     LAPGPRQADLMIVSGTVTDKMAPAVRRLYDQMPEPKYVISFGACSNSGGPYWDSYSVT
                     KGVDTIIPVDVYVPGCPPRPEALLQGILKLQEKIAAERIGDSVPRRYAGHRLASAAEL
                     TRPIVTAPGAVETGS"
     gene            3949..4662
                     /locus_tag="PKU75_10975"
     CDS             3949..4662
                     /locus_tag="PKU75_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81447.1"
                     /translation="MSSRIAVGGLLAAGLSVLGAAGAGAAPSGMPHTAATPSPTTASA
                     HGGYFRDAFNFQAPGWRGVVGRWSVKNGTLFAQGMPGRFSSVKHVNTYDDFDYEVRMN
                     RIGNSDGSAPNCLIIRANPNRRSEADLWLPGYYFCYTNNGKALAVAFDARGNRRELMD
                     YTPVKEIGTGGGYRTVRVQAFDHVFYFSVNGKEIGAFKDDLSSFGQVGAGFYVEPGKR
                     GSLALDYANLGPIKAAISG"
     gene            complement(4667..7597)
                     /locus_tag="PKU75_10980"
     CDS             complement(4667..7597)
                     /locus_tag="PKU75_10980"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HPF81448.1"
                     /translation="MTRVTAFMVRVDYRVLGALQAGPDAAVLRGQRARDVLALLLMRR
                     GRPVTPDAILDAVWGADAHTLDTSVVHTVIARLRRALGPHAIHRHDAGYQLHPRATLD
                     ADEFTRLISAARALVPDRRREIIELLQQALLLWSGPEAFGGVSDELVAGARPRLHELR
                     DAAVEELAERLLAGGTVGELTDAVLALQDLAEREPLRERAHELLMEGLYRLGRQGEAL
                     TVYDRLRRGLRDELGIDPGPATAAMQVRILNQDPSVGPAPRSVPPPRGVVPRPRTRTI
                     GREAELRQLADLAEAGRRLITIVGPGGVGKSRLLIEYAHTLPADARLVYAELPCGADV
                     SAAEVAETIARAAGLTLGGGDPVDTLVTALRDSEEIVLLDEAEWSVAATATLIERLLS
                     DGAGPRIVLTSRVPLGLVGESQLPLAPLAAPAPGATPDGVSVSPAVQLFVERLGDHLP
                     GLRLSEGDYARAAEITRRVDGLPLALEIVAGQSIASSVTELLSLVESPLDIEAVERDR
                     TARQRTLRETLAWSVDRLPEASRIVLRRLAVFSGTFDMAAAEAIVGHVPTIAAADIPA
                     AIRTLIREAQIQVVRRGGQARMRILRPSRAFLMEHLSEADETEDTMRRYRGWYAARWR
                     DQLLTDALIKEVATSYPDYHSALRDALRLRDAETLGDLAIVLCRYWFFVETGSEGVRL
                     VRAALATGVLSPRHTAILRLLEAALLPQDQGLEQRSVLDDLVPDLADDADWLGRLHIL
                     RSVGPYVHGDFPRALECAQEAVAHAQAQAEHHVPEALGAYAVMLAALGRATEAVAAGQ
                     EAWRLIAAAPSAVDLTQVVPKVALALIDSDHPREALEILDRALAQVEEELGLAPPSLF
                     TTNAGWAALGSGDPGSALLRFAQSLDQLAGGGDLLVTGEVLSGAGAAMAALSSDGAAD
                     VLDLADEHLRVAGAVLTPWQAAVVERHRAALPARAKSGLVLGPDFARGSMLIRHAADQ
                     HRSG"
     gene            complement(7609..8205)
                     /locus_tag="PKU75_10985"
     CDS             complement(7609..8205)
                     /locus_tag="PKU75_10985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81449.1"
                     /translation="MSSLIHPAIRHHLTPGGRVRLISLRGGEVALVRPVFDGLSERSR
                     WLRFHTGMPSVPPRYLEQLARVVPGHRHVLLAVAPHGPVGHAEWTRLPGTPSRAEVAV
                     AVVDAAHGQGVGLALVEELAHSAAAQGISQFTCSALRENRPVRQWLDRCGARVDPSDP
                     TECTFAITDLVAAIASHAGRATPPPENPLTSGDRGAVA"
     gene            complement(8269..10119)
                     /locus_tag="PKU75_10990"
     CDS             complement(8269..10119)
                     /locus_tag="PKU75_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPF81450.1"
                     /translation="MPSTQVNRVVIRFAGDSGDGMQLTGERFTADTASLGNDLSTLPN
                     FPAEIRAPQGTLPGVSSFQLHFADHDVLTPGDAPDVLVAMNPAALKANLRDLPRGATI
                     IADRDEFSKRNLSKVGYATNPLEDGSLESWHVHDLGLTSMTVEALAPFASLTRKEKER
                     AKNMFALGLLSWMYSRPIEGTRAFLQGKFGSKPDILAANLAALEAGWSYGETTEDFAS
                     PYEVAPAPMPAGTYRNVTGNTALAYGLVAGAHRAGLQLVLGSYPITPASDILHTLSGL
                     KRYGVMTLQAEDEIAGVGIALGAAFGGKLGVTSTSGPGLALKAETIGLAVSLELPLVV
                     VDVQRGGPSTGLPTKTEQADLLQAMFGRNGEAPVPVIAPRSPADCFDTALEAVRIATT
                     YRTPVVLLSDGYLANGAEPWQLPVVGELPDLTVQFADGPNAVDAQGGPVFQPYLRDPQ
                     TLARPWAIPGTAGLEHRIGGIEKADITGDISYDPDNHERMTQLRRAKVDGITVPDVVV
                     DDPSGTAEILVLGWGSTFGPITAAARAVRANGLSVATAHLRHLNPFPANLGEVLRAYP
                     KVLVPEMNSGQLAMLVRATFLVDAQSYARVRGLPFTTDELVEAISQLAAR"
     gene            10312..10614
                     /locus_tag="PKU75_10995"
     CDS             10312..10614
                     /locus_tag="PKU75_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81451.1"
                     /translation="MPADLPGGADLLSASALAVELDGLLFHRGTGRVDRAKQNAAVTA
                     GTWMLRFGWQETTESPCDAAASVIALARSLGLVWPTRPCRRRGCRVSAAVTVSPEL"
BASE COUNT         1693 a         3602 c         3722 g         1597 t
ORIGIN      
        1 tcggcgtcga tcgtcttgtc catcaggccg tcgacggact ggcggatcag atgccagccg
       61 gtccagatga tgttgcagcc cacgaggaag gcgatgatcg ggtcgagccg ctcccacttg
      121 gtgacagcca cgagcaacac cccgacgacg acgccgaccg aggtccacac atcggtgagc
      181 aggtgcttgc cgtcggcgac caacgtgatc gagttgtgcg cccgtccggc ccgcagcaac
      241 agcatcgcca ccgtgccgtt gagcaccgag gcgatcaccg aaatccccag tccgacaccg
      301 acattctgca aaccttgcgg gtgcaggaac cgttcgaccg aggtcagcag gatgaagacg
      361 gcggcgacga agatcatcac gccctcgacg accgccgaga aatactccgc cttcgagtgc
      421 ccgtagtggt ggtcgtcgtc cgggggcctg ctcgcgacgt gcaatgcgag cagtgcgaca
      481 aaggcggcca cgaggttgac aatcgactcg gcggcgtcgg agagcagacc gaccgacccg
      541 gtcaggacat aggcaccgaa cttcagcgcg atcgtcgcga tcgccgcggc aatggagagc
      601 caggcgaact tcgtcagatt cgcgcgctga gccgtcatac cggcattgtc cctcgcgaga
      661 tctgagcctg cgctgagacc ggcggcctag gctgcggccg ggtcgagggg cggtatgccg
      721 cgcgcgcgcc gggcggcctg cagggagtcg atcgtcagga gcagcaaggc gatccagacg
      781 atcccgaagc cgacccagag ccggccggac acgtgctcat gcaacacgat cacacccacc
      841 agcaattgca tcaccggcgt catgaactgg atgagcccga tggtcaccag cggcacccgc
      901 cgcgccgccg ccgcgaagag caggagcggg accgcggtga tgatccccgc gagggtcaac
      961 aaggtcgtat gaccggggct cgcgccatac gtcgcatccc cggcgccggc gacgaggatc
     1021 aggatgagcg cggcaaccgg cgtcagcacc gccgtctcgg cgaagagcga gtgccacgga
     1081 tccaaggcga ccccgagccg cttcttgagc agcccatagg tcccgaagga cagcgccagg
     1141 ctgatcgcga tccacgggaa gtgcccgccc tcgagcgcca gatagatcgc cgcgatcgcg
     1201 ccgaccgcca cggcgagcca ctgcagtggc cgcagcccct cgccgaggat gacgacgccg
     1261 agcgccaccg tcaccagcgg gttgaggaag tagccgaggg ccgcctcata cgtgtgtccc
     1321 gagagcacgg cgaagacgta gatcacccag ttggcagcga tgaccagggc cgccgcgatc
     1381 agtccgagcg acaggcgccg gctgcgggcc accgtgcgca tccacgccca atcccgccgc
     1441 accgcgagga tcgccaggca cagcagcatc gtccacacga tccggtgggc gaggatctcc
     1501 cacgccccgg ccggcttgag cgcgtggaag tagatcggga agatccccca gatgccatag
     1561 gccagcaggc cgtatgccgt gccccgccgg acgcggtcgg cctccgacga tcgcgcgacc
     1621 gccggcggcg gtgtcacccg acggtccagg tgtccctgcc ggcgagcagg gcggcgaggt
     1681 cggcgtcggt ggccggtgcg ccggcggtcg tgacggcggc ctcgatctgg ccgcggacga
     1741 ggtcgtcata tgtcggtcgc tcgacctggc ggaagatgcc catcggcacg tgggtcatcg
     1801 aggagtcgtc gatgcggctg agttcgaact gccggctcgg gtcggggtcg gtcgggtcgt
     1861 ggacgatgac ctcacggtcg ccggcctcgg cctccgggac gaagaccagc tttccgccct
     1921 cgcgcaccag caccttgcga tcgtcctccg cgccgaccgt gaccggctgc ccctgcgcga
     1981 ggtggacgag acgagcctgc tgctggtcgc ggtccttgat cgcatcgaac gcgccgtcgt
     2041 tgaagatcgg gcagttctga tagatctcga ccagggccgt gccgcggtgt gccgcagcgg
     2101 ctttgaggat ctcggtcaag tgggcgcggt cgttgtcgag gctgcgggcg acgaaggtcg
     2161 cctccgcccc gagcgccagg ctgaccgggt tgaacggggt gtcgaccgac ccggcaggcg
     2221 tggacttggt gatcttgccc tgctcggagg taggggagta ctgccccttg gtcagcccat
     2281 agatccggtt gttgaacagc agaatggtca tattgacgtt gcggcgcagc gcgtggatca
     2341 ggtggttgcc gccgatggag agggcgtcgc cgtcaccggt gacgacccac accgacaggt
     2401 ccggccgcga tgtcgcgagc ccggtggcga tcgccggcgc ccgcccgtgg atggagtgca
     2461 tgccgtaggt gtcgaggtaa taggggaacc gcgacgagca gccgatcccg gagacgaaga
     2521 cgatgttctc cttgcgcagc cccagttcgg gcaggaagga ctgcacggtg ttgaggatga
     2581 tgtagtcgcc gcagccgggg caccagcgca cctcctggtc ggaggtgtag tccttcttcg
     2641 tcagcttcgc gtccccgccc agccggtgca tgccctccag gccgcgaggg agcggcatac
     2701 cgaggtcgac cgactcccca cggtgctgct cgatggggcg ctcgtcggtg gtggtgccca
     2761 gcggtgctgc cattgcccaa aggactttcg tctcgaccag tgcgggaatt cccacacaca
     2821 accgttcgat tttacgcgct cctggagctt ccggcggcgg acgcgcccgg gatccggacg
     2881 tgtggccagt gtgttgacta ggctgcgtgg cgtggaggca tatgtggtgg cagcggccgt
     2941 cctcggagcg ggcgtgctgc tctttgtcgc ggcgatggcc gcgcgctggc tgttcgcgcc
     3001 acgcacccgc acggtggcga gcggcacgac ctacgaatcg ggagtcgacc cggtgggcac
     3061 cggctgggcg cagacccagg tgcgctatct cgtctatgcc ttcctctatg tcatcttcgc
     3121 cgtcgacgcc gtctacctct ttccgtgggc gctcgtcgcg cgcggccacc tgggccgcgc
     3181 ctcgctcgtc gaggtcggcg tcttcatcgg catcatcctc atcggactgg cccacgccgc
     3241 ccggcgcggc ctgctgaaat ggacctagga gccagccgta tgccggtcga tctccccact
     3301 ccccgcgtcg ggccggtcgg tgcgcacgca ccgaagccga tgcgggtggt cctcaactgg
     3361 gggcgccgct acagcctgtg ggtgttcaac ttcgggttgg cctgctgcgc gatcgagttc
     3421 atcgccgcct ccatgggccg gcacgacttc atgcgcctcg gcgtcatccc gctcgcgccc
     3481 ggcccgcggc aggccgacct gatgatcgtc tccggcaccg tcaccgacaa gatggccccg
     3541 gccgtgcgcc ggctctatga ccagatgccc gagccgaaat acgtcatctc cttcggtgcc
     3601 tgctccaatt ccggtgggcc ctactgggat tcgtactcgg tgaccaaggg tgtcgacacg
     3661 atcatccccg tcgacgtcta cgtcccgggc tgcccgcccc gcccagaggc gctgctgcag
     3721 ggcattttga aactgcagga gaagatcgcc gccgaacgca tcggcgacag cgtcccgcgc
     3781 cggtatgccg gccaccgcct cgcctccgct gcggaactca cccggccgat cgtcaccgcg
     3841 ccgggggccg tggagaccgg ctcctgacag atcgatgaca gggcacccct gaagagtggt
     3901 cgcagtcccg ggcaatccgc ccgggaggac cacaacggag gaaatcccat gtcttcacga
     3961 atcgccgtcg gcggtttgct cgccgccgga ctgagcgtcc tcggcgccgc aggggccggc
     4021 gccgcaccct cgggtatgcc gcatacggcc gccacgccca gccccaccac cgcctcggcc
     4081 cacggaggct acttccgtga cgccttcaac ttccaggcac ccggctggcg gggtgtcgtc
     4141 ggccggtgga gcgtcaagaa cggcacgctg ttcgcgcagg gaatgccggg ccgcttctcg
     4201 tcggtcaagc acgtcaacac ctatgacgac ttcgactacg aggtgcggat gaaccggata
     4261 ggcaatagcg acggcagcgc accgaactgc ctgatcattc gcgccaatcc caaccggcgc
     4321 agcgaggcgg acctgtggct gccgggctat tacttctgtt acaccaacaa cggcaaggcg
     4381 ttggccgtcg ccttcgacgc ccgcggcaac cggcgcgagt tgatggacta cacgccggtg
     4441 aaagagatcg gcacgggcgg cggctatcgg accgtgcgcg tgcaggcctt cgaccacgtc
     4501 ttctacttca gcgtcaacgg caaggagatc ggcgccttca aggacgactt gtcgagcttc
     4561 ggccaggtcg gtgccgggtt ctatgtcgag ccgggcaagc gcggcagcct cgccctcgac
     4621 tacgccaatc tcggaccgat caaggccgca atctccggct gaccggtcag ccggagcggt
     4681 gctggtccgc cgcgtggcgg atcagcatcg acccgcgggc gaagtccggc ccgagcacga
     4741 ggccggactt cgcccgcgcg ggcagcgccg cccggtgccg ctcgaccacc gccgcctgcc
     4801 acggcgtcaa taccgccccg gcgacacgca ggtgctcatc ggcgagatcg agcacgtcgg
     4861 ctgcgccgtc actgctcaag gccgccatgg cagcccccgc gccggagagc acctctccgg
     4921 tcacgagcag gtcgccgcca ccggcgagct gatccagtga ttgcgcgaat cgaagcagcg
     4981 cggagcctgg gtcgccgctg cccaacgcgg cccagccggc gttggtggtg aacaacgacg
     5041 gcggagccaa gcccagctcc tcctcgacct gcgccagcgc ccggtccagg atttccaacg
     5101 cctcccgcgg atggtccgaa tcgatcagtg ccagagcaac tttcgggacc acctgggtca
     5161 ggtccacggc gctgggcgcg gcggcaatca accgccaggc ctcctggccc gctgccaccg
     5221 cctcggtggc ccgaccgagg gcggcgagca tcacggcata cgcgccgagg gcctccggca
     5281 cgtggtgctc cgcctgagcc tgcgcgtgcg cgaccgcttc ctgggcgcac tccagcgcgc
     5341 gcgggaagtc accgtgcaca tacgggccga ccgagcgcag gatgtgcagc cggcccagcc
     5401 agtccgcgtc gtcggccagg tccgggacga gatcgtcgag gaccgatcgc tgctccagtc
     5461 cctggtcctg cggcaggagc gccgcctcga gcaagcgcag gatcgccgta tgccgcgggc
     5521 tgagcacacc ggtggccagg gccgcgcgca ccaggcgcac cccctcgctg ccggtttcga
     5581 cgaagaacca gtaccggcac aagacgatgg ccaagtcacc gagggtttcg gcgtcgcgca
     5641 ggcgcaacgc atcgcgcaag gccgagtgat agtccggata ggacgtcgcg acctccttga
     5701 tgagggcgtc ggtcagcaac tgatcgcgcc agcgtgcggc ataccatccg cggtagcgtc
     5761 gcatcgtgtc ctcggtctcg tcggcctcgg acaagtgctc catcaggaat gctctggagg
     5821 ggcgcaggat gcgcatgcgg gcctggccgc cacgccggac gacctggatc tgggcttcgc
     5881 ggatcagcgt ccggatggcc gccgggatgt cggctgcagc gattgtcggc acatgaccga
     5941 cgatggcttc ggccgccgcc atgtcgaagg ttccggaaaa aacagccaac cgccgcaaca
     6001 cgattcgcga cgcctcggga agccgatcga cgctccaggc gagggtttcg cgcagcgtgc
     6061 gctggcgcgc ggtgcggtcc cgttcgaccg cctcgatgtc gaggggcgac tcgaccaggc
     6121 tgagcagctc ggtgacggat gaggcgatgg actgcccggc aacgatctcc agcgccagcg
     6181 gcaatccgtc cacccggcgg gtgatctccg cagctcgcgc atagtcgcct tccgacaggc
     6241 gtaggccggg caagtggtcg ccgaggcgct cgacgaagag ttggacagcg ggcgagacgg
     6301 atacgccatc cggcgtcgcc ccgggtgccg gcgcggcgag cggtgcgagg gggagttggc
     6361 tctcgccgac gagtccgagc ggcactcggg aggtcaggac gatgcgtggg ccggccccgt
     6421 cgctcagcag gcgttcgatc aacgtggccg ttgctgccac cgaccactcg gcctcatcga
     6481 gcaggacgat ttcctcgctg tcgcggagtg ctgtgacgag cgtgtccacc ggatcgccgc
     6541 caccgagagt gaggcccgcg gcgcgcgcaa tggtctcggc aacttcggcc gcggagacat
     6601 ctgcgccaca aggtagttcg gcataaacga ggcgcgcgtc cgctggcagg gtgtgtgcgt
     6661 attcgatgag cagacggctc ttgccgacgc cgccggggcc gacgatcgtg atcagccggc
     6721 gaccggcttc ggcgaggtcg gcgagttgac gcagctcggc ctcccggccg atggtgcgcg
     6781 tgcgcggcct gggcacgacg ccgcgtggcg gcggaacgga tcgcggtgcc ggcccgacgc
     6841 tggggtcctg gttgagaatc cgcacctgca tggcggcagt ggcgggaccg ggatcgatgc
     6901 ccagttcgtc acgcagtcca cgccgcagcc ggtcgtagac ggtgagcgct tcaccctgcc
     6961 ggccgagccg atacaggccc tccatgagca gttcgtgcgc ccgctcgcgc aatggctcgc
     7021 gctcggcgag atcctgcaac gcgagaactg catcggtgag ttcgccgacc gtgccgcctg
     7081 ccagcagtct ttcggcgagt tcctccactg cggcgtcgcg cagttcgtgc agccggggtc
     7141 gtgcgccggc cacgagctcg tcgctcaccc cgccgaaggc ctccgggccg gaccagagca
     7201 gcagcgcctg ctgcagaagt tcgatgatct cgcgccggcg atcggggacg agggcacggg
     7261 cggccgagat caacctggtg aactcgtcgg cgtcgagggt cgcccgggga tggagctggt
     7321 agccggcgtc gtgacggtgg atggcgtgcg gtcccagagc ccgacggagc cgggcgatga
     7381 cggtgtggac cacgctggtg tcaagggtgt gagcatccgc gccccagacg gcgtcgagga
     7441 tggcatcggg cgtcaccggc cggccgcggc gcatgagaag tagcgcaagc acgtcgcggg
     7501 cgcgctgccc gcgcagcacc gcggcgtcgg gtccggcttg gagtgccccg agtacgcggt
     7561 agtccaccct caccatgaaa gcagtcacgc gcgtcacgcg cagcgagttt acgcaactgc
     7621 tccacgatcg ccggatgtca gcgggttctc cggtggaggg gtcgcgcgcc ccgcgtgtga
     7681 cgcgattgcg gcgaccagat cggtgatcgc gaacgtgcac tccgtcgggt cgctcggatc
     7741 aaccctcgcg ccgcaccggt ccagccattg gcgcaccggc cggttctctc gtagagccga
     7801 gcaggtgaac tgcgaaattc cctgggcggc agcggaatgt gccagctctt cgacaagagc
     7861 caagccgact ccctggccgt gcgccgcgtc cacgaccgcg acggcaacct ccgcccgaga
     7921 cggggtcccc ggcaggcgag tccactccgc gtgccccacg gggccgtggg gcgccacggc
     7981 gagcagcacg tgccggtggc ccgggacgac tcgcgcgagt tgctcgaggt agcggggcgg
     8041 gaccgacggc attccggtat ggaagcgcag ccagcgggag cgctcggaca ggccgtcgaa
     8101 gacgggacgg accaaggcga cctcgccgcc gcgcaggctg atcagccgga cgcggccacc
     8161 gggcgtgagg tgatggcgga tggcgggatg gatgagactg gacatcgccc cagcctgaat
     8221 gcgcggtctg tcgcgcctct gtcgtggacc ggtcagacgc ggagggcgtc agcgcgcggc
     8281 gagttggctg atcgcctcga cgagttcgtc agtggtgaac gggaggccgc gcacgcgggc
     8341 atacgactga gcatccacca ggaacgtggc gcggacgagc atcgcgagct ggccgctgtt
     8401 catctccggc acaagaactt tcggatacgc ccgaagcacc tcgccgaggt tggcggggaa
     8461 ggggttgagg tggcgcaggt gtgccgtcgc gacgctgagg ccgttcgccc gcacagcccg
     8521 ggccgcggcc gtgatcggtc cgaacgtcga accccacccg aggaccagga tctccgcagt
     8581 gccactcgga tcgtcgacga cgacgtccgg caccgtgatg ccgtcgacct tggcccgccg
     8641 cagctgggtc atccgctcgt ggttgtcggg gtcataggag atgtcgccgg tgatgtcggc
     8701 cttctcgatg ccgccgatgc ggtgttcgag gccggcggtg ccggggatcg cccacggccg
     8761 ggccagggtc tgcgggtcgc gcagataggg ctggaagacc ggcccgccct gggcgtcgac
     8821 cgcattgggc ccgtcggcga attgcactgt caggtcgggc aattcgccga cgaccggcag
     8881 ttgccacggc tcggcaccat tggcgagata gccatcggag agcagcacca ccggggtccg
     8941 ataggtcgtc gcgatgcgca ccgcctcgag ggcggtgtcg aaacaatcgg ccggggagcg
     9001 gggcgcgatg actggcacgg gtgcctcgcc gttgcggccg aacatcgcct gcagcaggtc
     9061 ggcctgttcg gtcttggtgg gcaggccggt cgagggcccg ccgcgctgga cgtcgacgac
     9121 gacgagcggc agctccaacg agaccgccaa cccgatcgtc tcggccttca aggcgaggcc
     9181 ggggcctgag gtcgacgtga ccccgagctt gccgccgaag gcggccccca gggcgatccc
     9241 gaccccggct atctcgtcct cggcctgcag ggtcatcacg ccgtaacgct tcaagcccga
     9301 gagggtgtgc aggatgtccg aggcgggagt gatcggatag gagccgagga ccagttgcag
     9361 tccggcgcgg tgggcgccgg cgaccaggcc gtacgccaac gcggtgttgc cggtgacatt
     9421 gcgataggtg ccggcgggca tgggtgccgg ggcgacctca tacggcgacg cgaagtcctc
     9481 cgtcgtctcg ccgtagctcc aacccgcctc cagcgcagcc agattcgccg cgagaatgtc
     9541 cggctttgac ccgaacttgc cctggaggaa ggcccgggtg ccctcgatgg gccgcgagta
     9601 catccacgac agcaggccga gcgcgaacat gttcttcgcg cgttccttct ccttgcgcgt
     9661 cagcgacgcg aagggcgcca gcgcctcgac ggtcatgctc gtcaggccga ggtcgtgcac
     9721 atgccaggac tcgagcgagc cgtcctcgag cggattggtc gcatagccga ccttgctcag
     9781 attccgcttg gagaactcgt cccggtcggc gatgatcgtc gccccgcgcg gcaggtcgcg
     9841 caggttcgcc ttcagcgccg ccgggttcat cgccacgagg acatccggcg catcaccggg
     9901 ggtgagcacg tcgtgatcgg cgaagtgcag ctggaagctg gacaccccgg gcagcgtgcc
     9961 ctgcggcgca cggatctcgg cggggaagtt cggcagcgtc gacagatcgt tgccgagact
    10021 cgcggtgtcc gcggtgaagc gctcgcccgt caactgcata ccgtcgccgg aatccccggc
    10081 gaagcggatc acgacgcgat tgacctgggt gctgggcata tcgcccatag tagggcgacc
    10141 taacccgtcc gcgccccgcg tgcgtcttcg cctcctggtg gtcgtcgtta actgagtcac
    10201 gtcatgtgtg tgggagtgac gtcatggcgt cactcccaca cagatgacga cgacatccac
    10261 aggcgcgcga gcgactttgc gccgtccaca gggttgcggc acgcccttgg cgtgccggcg
    10321 gacctgcccg gtggcgccga cctgctgagc gcctcggcgt tggccgtcga actcgacggc
    10381 ctgctctttc accggggcac aggacgtgtg gacagggcca agcagaacgc tgcggtgacg
    10441 gcgggcacgt ggatgttgcg ctttgggtgg caggagacca ccgagtcacc gtgcgacgcg
    10501 gcggcatcgg tgatcgcgct cgcccgttcg ctcggactgg tgtggccgac ccgcccctgt
    10561 cgccgccgcg gttgccgcgt gagcgccgcg gtgaccgtgt cacctgagct ttag
//
LOCUS       DAOPJG010000117        10439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6698_length_10439_cov_7.741911, whole genome shotgun sequence.
ACCESSION   DAOPJG010000117 DAOPJG010000000
VERSION     DAOPJG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10439
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6698_length_10439_cov_7.741911"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /locus_tag="PKU75_11000"
     CDS             <1..1844
                     /locus_tag="PKU75_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HPF81452.1"
                     /translation="MNRIGGKSNTGEGGEDADRLHDPVRRSAIKQVASGRFGVTSDYL
                     TNAADIQIKMAQGAKPGEGGQLPAHKVYPWVAKTRHSTPGVGLISPPPHHDIYSIEDL
                     AQLIHDLKNANPAARIHVKLVSEVGVGTVAAGVSKAHSDVVLISGHDGGTGASPLTSL
                     KHAGAPWELGLAETQQTLVLNGLRDRIVVQVDGQLKTGRDVVIAALLGAEEFGFATAP
                     LVVSGCIMMRVCHLDTCPVGIATQNPELRSRYTGRPEFVETFFEYIAQEVREHLAALG
                     FRSIAEAIGQIGALDTTAAVHHWKTDGMDLTPILTEAVPGDGVTRYQSVTQDHGLEKA
                     LDHQLISIARTALDDGDQARGQLAIRNVNRTVGTMLGHEVTSRQVAGLADGSIDITLT
                     GSAGQSFGAFLPTGVTLRLVGDANDYVGKGLSGGKLVVSPHPSSVFRAEENVIAGNVI
                     GYGATSGEIFLRGIAGERFCVRNSGATAVVEGVGDHGCEYMTGGVALILGRIGRNFAA
                     GMSGGVAYVLDLPAHRVNGEFVDIVALRDEDEDLVRTLLQAHADATGSPVAADLLADW
                     ESAKARFSLILPRDYARVLQVRADAEAEGIAPESDIVWERIMEAARG"
     gene            1837..3294
                     /locus_tag="PKU75_11005"
     CDS             1837..3294
                     /locus_tag="PKU75_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HPF81453.1"
                     /translation="MADAQGFLKTRERELPERRPVPVRIKDWKEVYEAQDVAQLQRQA
                     GRCMDCGIPFCHSGCPLGNLIPEWNDMAWRGDWRGAIERLHATNNFPEFTGRLCPAPC
                     ETACVLGINQPAVTIKQVEVTTIDRAWEAGSVPPQPPDRLSGKTVAVIGSGPAGLAAA
                     QQLTRAGHTVAVFERADKPGGLLRYGIPEFKMEKAVLDRRLGQMEAEGTRFRCGVDVG
                     GSLTGQQLRARYDAVVLAIGSTLPRDLPVPGREADGVVQAMDFLPQANRVALGERVAD
                     QITATGKDVIVIGGGDTGADCIGTSHRQGARSVTSLEIMPQPGPERPGNQPWPTYPMI
                     FRVASAHEEGGERLYAVSTQEITKDADGRVAALRVQDVRLGEKGFEPVEGTERELPAQ
                     LVLLAMGFVGPQQQGLVEQLEVELDERGNVKRDKDFQTTVPGVFVAGDAGRGQSLIVW
                     AIAEGRAAARGVDEYLMGASQLPRPINPTDRPLTV"
     assembly_gap    3320..3419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3517..4959
                     /gene="pyk"
                     /locus_tag="PKU75_11010"
     CDS             3517..4959
                     /gene="pyk"
                     /locus_tag="PKU75_11010"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPF81454.1"
                     /translation="MRRAKIVCTIGPATAAPERLAELVAAGMDVARMNLSHGSYEDHA
                     KVYANVREASNASGRAVGVLVDLQGPKIRTMRFSGGPIELIAGDTFTITTRDVDGTQE
                     IVGTTFKGLPGDVEPGDTLLIDDGKVELVATAVTDTDVTCEVVQGGTVSNNKGINLPG
                     VAVSVPALSEKDEEDLRWALHLAADFIALSFVRSAEDIRRVHEIMDEEGLRLPVIAKI
                     EKPQAVENLESIVAAFDGIMVARGDLGVELPLEQVPLVQKRAIEIARRNAKPVIVATQ
                     VLESMIENPRPTRAEASDAANAVLDGADALMLSGETSVGAHAIGAVKTMARIIENTEE
                     TGFERISALTAPPQTVSGAITRAAVDIAQLVGARYLVTFTTSGSSARAMSRLRPYRPM
                     LAFTPSPATRSQLALTWGVETFLVPNVTHTDDMAFQVDEALMRDKRCPEGERVVIAAG
                     SPPGVSGTTNALRVHSVGDAINHRGAFAHL"
     gene            5027..5107
                     /locus_tag="PKU75_11015"
     tRNA            5027..5107
                     /locus_tag="PKU75_11015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5060..5062,aa:Leu,seq:caa)
     gene            5162..5785
                     /locus_tag="PKU75_11020"
     CDS             5162..5785
                     /locus_tag="PKU75_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPF81455.1"
                     /translation="MSNEAKATTTAAGSAPTAPRIVVAEDEALIRLDLVEMLGEAGYD
                     VVGQASNGEQAVELAVQTRPDLVIMDVKMPVMDGLAAADEIGKQRLCPVIMLTAFSQT
                     ELVERARDAGVMAYIVKPFTAADLAPAINIALSRWSELKELEKEVADLGEALETRKAV
                     ERAKGVLMKKLKLSEAEAFRWIQKTAMDRRMGMKDVAEAVIAGIDKA"
     gene            6007..6612
                     /locus_tag="PKU75_11025"
     CDS             6007..6612
                     /locus_tag="PKU75_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81456.1"
                     /translation="MARPHLNVTKALPGDAGDLSALWIEWALCSGMSHDLAARAGDVA
                     RITEALERPVTTVLLARLDHRPVGFAVVNTRNHGLLDPAGLAIDELYVIPEGRRQGVA
                     AGLLGAVATMAESEGHDLVFANVAATDKLSNRYLARLGFGTTITRRVVPTAVLRRKLS
                     GADTASQEILLGKRRTLRARSRGAVGSAAPARAISLRRTAI"
     gene            complement(6616..7065)
                     /locus_tag="PKU75_11030"
     CDS             complement(6616..7065)
                     /locus_tag="PKU75_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HPF81457.1"
                     /translation="MTDNAIFSSSADVLAAVNAMSKGTLADRMGIEITELSKDRVVGT
                     MPVAGNQQPYGLLHGGASVVLAETLGSLGSAMHAGPARAAVGIDINATHHRAARSGIV
                     TGVATVLSAGKTMVSYEVVITDDQGRRCCTSRITCLLRDLPPGAKAE"
     gene            7085..9754
                     /gene="polA"
                     /locus_tag="PKU75_11035"
     CDS             7085..9754
                     /gene="polA"
                     /locus_tag="PKU75_11035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPF81458.1"
                     /translation="MSRLLLLDGHSLAYRAFYALPAENFSTKTGQHTNAVYGFTSMLI
                     NVLRDEDPTHIAVAFDHSRQTFRMEEYAEYKAGRSATPSEFSGQLPLLREVLDALQIR
                     WLEVPGFEADDIIATLTTRAAEQDMEVVICSGDRDAFQLVTDTVTLLYPVRGVSEVWR
                     MTPAKVEEKYGVPPQRYGDLAALVGESSDNLPGVPGVGPKTAAKWIQQYDGLSGIVAH
                     VDQIGGKVGAALRDNLEQVLRNRRLNQLVRDLDTGLTVDDLQRRMWDRERVHEVFDAL
                     EFRVLRERLFTTFDTTAVEAEGGFDVETRSLAGGDVGEWLAGPGRTGEIGVHVVGSWA
                     RGDGDVGALALAGSEGPAVFIDVTQLDPDGEAALGHWLADPEVPKFVHDAKGPIHALH
                     ARGWELGGLALDTALAAYLVKPDQRDFDLADLVVRMLGRDLRAETGAAEGMLDFGADD
                     QAEIADAGVRARAVLDLGHTLAEQVEKAGGTRLLREVELPLVGVLAEMEQTGIAVDLT
                     HLEALEKDFADAVERAQEDAYDAIGGEQINLGSPKQLQVVLFDTLAMPKTKRTKTGWT
                     TDAEALADLYTKTEHPFLEALLRHRDATRLRVTVEGLLKSVSDDGRIHTTYEQTIAAT
                     GRLSSKDPNLQNIPIRTDEGRRIRESFVVGPGYESLMSVDYSQIEMRIMAHLSGDAGL
                     IEAFRSGEDLHRFVGSRVFAVAPQDVTPAMRSKIKAMSYGLAYGLSAFGLSRQLTIPR
                     EEASALMEEYFARFGGVRDYLRDVVADERATGYTETMLGRRRYLPDLTSDNRQRREMA
                     ERMALNAPIQGSAADIMKVAMINAHNALRDAGLNSRLLLQVHDELVVEVAPGEAEQVE
                     ALVRREMGSAAGEIDVPLDVSVGIGRSWHDAGH"
     gene            9799..>10439
                     /locus_tag="PKU75_11040"
     CDS             9799..>10439
                     /locus_tag="PKU75_11040"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005272710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HPF81459.1"
                     /translation="MPADHPTILATSGGYRFGTRTRMEFNHLVHHAVELAGVSGHAPR
                     VCLVGTASGDQRWFAAEMDEAARVAGFDLTHLHLFPMPNVEDIEGHLMAQDVVWVNGG
                     SVANLLAVWDVHDLRPVFRRAWEAGVVLSGVSAGSICWYDGGCTDSYGPDLRAVTNGL
                     GLLPYGNGVHYDSEEQRRPTVHRLVGDGTLALTHCTDDGVGIVYHGTDLVEAVT"
BASE COUNT         1672 a         3696 c         3475 g         1496 t
ORIGIN      
        1 ccatgaaccg gatcggcggc aagtcgaaca ccggcgaggg cggcgaggac gccgaccggc
       61 tgcacgaccc ggtccggcgc tccgcgatca agcaggtcgc ctcgggaagg ttcggcgtca
      121 cctccgacta cctgaccaac gccgccgaca tccagatcaa gatggcccag ggcgccaagc
      181 ccggcgaggg cggccagctg cccgcccaca aggtctaccc gtgggtggcc aagacccgcc
      241 actcgacacc cggtgtcggc ctgatcagcc cgccaccgca ccacgacatc tactccatcg
      301 aggatctcgc ccagctgatc cacgacctga agaacgccaa tccggcggcg cggatccacg
      361 tcaagctcgt ctccgaggtc ggcgtcggca cggtcgcggc gggggtgtcc aaggcgcact
      421 ccgacgtcgt gctcatctcc gggcacgacg gcggcaccgg ggcctcaccg ctgacctcgc
      481 tcaagcacgc cggcgctccc tgggaactcg gcctcgccga gacccagcag acgctggtcc
      541 tcaacgggct gcgcgaccgg atcgtggtcc aggtggacgg gcagctgaag acggggcgtg
      601 atgtcgtcat cgccgcgctt ctcggtgccg aggagttcgg gttcgccacc gccccgctgg
      661 tcgtcagcgg ctgcatcatg atgcgggtct gccacctcga cacctgcccg gtcggcatcg
      721 cgacccagaa ccccgaactg cgctcccgct acaccggacg acccgaattc gtcgagacct
      781 tcttcgagta catcgcccaa gaggtgcgcg agcacctggc cgcactcggc ttccgctcca
      841 tcgccgaggc catcggccag atcggcgccc tcgacaccac cgccgcggtc caccattgga
      901 agaccgacgg catggacctg acgccgatcc tgaccgaggc cgtccccggc gacggtgtca
      961 cgcgctacca gagcgtcacc caggaccacg gactcgagaa ggcactcgac catcagctga
     1021 tctcgattgc ccgcaccgcc ctcgatgacg gcgaccaggc gcggggccag ttggcgatcc
     1081 gcaatgtcaa ccggaccgtg ggcacgatgc tcggtcatga ggtcaccagc cggcaggtcg
     1141 ccggcctggc cgacggcagc atcgacatca ccctcaccgg ttcggccggc cagtccttcg
     1201 gcgcgttcct gcccaccgga gtgacgctgc gcttggtcgg cgatgcgaac gactatgtcg
     1261 gcaaggggct ttcgggtggc aagctggtcg tcagccccca ccccagctcg gtcttccgcg
     1321 ccgaggagaa cgtcatcgcc ggcaatgtca tcggctacgg cgccaccagc ggcgagatct
     1381 tcctgcgcgg catcgccggt gagcgcttct gtgtccgcaa ctccggcgcg accgccgtcg
     1441 tcgagggcgt cggcgaccac gggtgcgagt acatgaccgg tggagtggcg ctcatcctcg
     1501 ggcgcatcgg gcgcaacttc gccgccggca tgtccggtgg ggtcgcctat gtcctggacc
     1561 tgccggccca tcgcgtcaac ggcgagttcg tcgacatcgt cgcgctgcgc gacgaggacg
     1621 aggatctcgt ccgcaccctg ctgcaggccc acgcggacgc caccggctcc ccggtcgcgg
     1681 cggacctgct ggccgattgg gagagcgcca aggcccgttt ctccctcatc ctcccgcgcg
     1741 actatgcacg ggtgttgcag gtgcgagcgg acgccgaggc agagggcata gcgcccgagt
     1801 ccgacatcgt gtgggaacgg atcatggagg cggcacgtgg ctgacgcaca aggttttctc
     1861 aagacccgcg agcgcgagct tcccgagcgc cgcccggtgc cggtccgcat caaggactgg
     1921 aaagaggtgt acgaggcgca ggacgtcgcc cagctgcaac ggcaggccgg gcgctgcatg
     1981 gactgcggca tcccgttctg tcactccggc tgccccctcg gcaatctcat ccccgagtgg
     2041 aacgacatgg cctggcgcgg cgactggcgc ggcgccatcg agcggctgca cgcgacgaac
     2101 aacttcccgg agttcaccgg gcggctgtgt cccgccccat gcgaaaccgc gtgcgtcctc
     2161 gggatcaacc agcccgccgt gacgatcaag caggtcgagg tcaccaccat cgaccgcgcc
     2221 tgggaggccg gctcggtccc gccgcagccg ccggatcgcc tgtccggcaa gaccgttgcc
     2281 gtcatcggct ccggccccgc gggcctggct gccgcgcagc aactgacgcg ggccggccat
     2341 acggtcgccg tcttcgaacg cgccgacaag cccggcgggc tgctgcgcta cggcatcccc
     2401 gagttcaaga tggagaaggc ggtcctggac cggcgcctgg gtcagatgga ggccgaaggc
     2461 acccgcttcc ggtgcggcgt cgacgtcggt ggctcgctca cgggccagca gctgcgtgcg
     2521 cggtatgacg cggtggtcct cgccatcgga tcgacgctcc cgcgtgacct gcccgtgccg
     2581 ggccgggagg cggacggcgt ggtgcaggcg atggattttc tgccgcaggc caaccgggtc
     2641 gccctcggcg agcgggtcgc cgaccagatc accgccaccg gcaaggacgt catcgtcatc
     2701 ggcggcggtg acaccggcgc cgactgcatc ggcacctccc atcgacaggg cgcacgatcg
     2761 gtgacgagcc tggagatcat gccccagccg gggccggagc gcccgggcaa ccagccgtgg
     2821 ccgacctatc cgatgatctt ccgggtcgcc agcgcccacg aagagggcgg ggagcggctg
     2881 tatgccgtct ccacccagga gatcacgaag gacgccgacg gccgggtcgc cgccctccgg
     2941 gtccaggacg tgcggctggg ggagaagggg ttcgagcccg tcgagggcac cgagcgtgag
     3001 ctgccggcgc aactggtcct gctcgccatg ggcttcgtcg gcccgcagca gcagggcctg
     3061 gtcgaacagc tggaggtgga gctggacgag cgcggaaatg tcaagcggga caaggacttc
     3121 cagaccactg tgcctggcgt cttcgttgcc ggtgacgccg gacgcggcca atccctgatc
     3181 gtctgggcga tcgcggaggg ccgggccgcc gccagagggg tggatgaata cctcatgggc
     3241 gcctcgcagc tgccgcgtcc gatcaacccc accgatcgtc cgctgacggt ctgaaccaca
     3301 cgtccgcccg cggggctgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3421 cccgcggggc tgacgacggg ctcaggcgct ggccaacggg cctgccgcgg ccgcgacgcg
     3481 gtcatgagta agcgtttaca cggttaccat tgacctatgc gtcgagcgaa gatcgtctgc
     3541 accatcggcc cggccacggc cgctcccgag cgcctggccg agctggtggc ggcgggcatg
     3601 gacgtcgcga ggatgaacct gtcccacggc agctacgagg accacgcgaa ggtctacgcc
     3661 aacgttcgcg aggcatccaa cgccagcggc cgcgccgtgg gcgtgctcgt ggatctccag
     3721 ggaccgaaga tccggaccat gcgcttcagc gggggtccca tcgagctgat cgccggcgac
     3781 acgttcacca tcaccacgcg cgatgtcgac ggcacccagg agatcgtcgg caccactttc
     3841 aagggcctgc ccggcgatgt cgagcccggc gacaccctgc tcatcgatga cggcaaggtc
     3901 gaactggtcg ccaccgcggt gaccgacacc gacgtcacct gcgaggtcgt ccagggcggc
     3961 acggtgtcca acaacaaggg catcaacctg cccggcgtgg cggtcagcgt gccggccctg
     4021 tccgagaagg acgaggagga tctgcgctgg gccctgcacc tggccgccga tttcatcgcc
     4081 ctgtccttcg tccgttcggc cgaggacatc cgccgcgtcc acgagatcat ggacgaggag
     4141 ggtttgcggc tgccggtgat cgccaagatc gagaagccgc aggccgtcga gaacctcgaa
     4201 tccatcgtcg cggccttcga cgggatcatg gtcgcccggg gcgacctcgg cgtcgaactg
     4261 ccgctggagc aggtcccgct cgtgcagaag cgcgccatcg agatcgcgcg ccgcaacgcc
     4321 aagccggtca tcgtcgccac ccaggtgctc gagtcgatga tcgagaaccc ccgccccacg
     4381 cgagccgagg cctccgatgc cgccaacgcc gtcctcgacg gcgcggacgc cctcatgctc
     4441 tccggcgaaa ccagcgtcgg ggcccacgcg atcggcgcgg tcaagacgat ggcgcgcatc
     4501 atcgagaaca ccgaggagac cgggttcgag cgcatctcgg ccctgaccgc gccgccgcag
     4561 accgtcagcg gagccatcac acgcgcggcg gtcgacatcg cccagctcgt cggcgcccgc
     4621 tatctggtca ccttcaccac gtcggggagt tcggcgcgcg cgatgtcgcg gctgcggcca
     4681 taccgcccga tgctcgcctt caccccgagc cccgccaccc gcagccaact cgccctcacc
     4741 tggggagtgg agaccttcct cgtccccaat gtcacgcaca ccgacgacat ggccttccag
     4801 gtcgacgagg ccctgatgcg cgacaagcgg tgccccgagg gcgagcgcgt ggtcatcgcc
     4861 gccggttccc cgcccggcgt cagcgggacg acgaacgcct tgcgcgtgca ctcggtcggg
     4921 gacgccatca accaccgagg ggcgttcgcg cacctgtgac aagagtccac cgcagcccgg
     4981 gcgtccgctg tcgcggaacg tcaccggtat gctgtgcggg cacctcgccg gggtggtgga
     5041 atggcagaca cggagcactc aaaatgcttt gccgcaaggc gtgcgggttc aaatcccgct
     5101 cccggcacgt gaacgacgag cgaccacgcg ggtcggctcg aggacagatg aggtacgcac
     5161 gatgagcaat gaggccaagg ccacgacgac cgctgccggg agcgctccca ccgcgccccg
     5221 catcgtcgtg gctgaggacg aagcgctgat ccggctcgac ctggtcgaga tgctcggcga
     5281 ggccgggtat gacgtggtcg ggcaggccag caacggcgag caggccgtcg agctggccgt
     5341 gcagacccgg cccgacctgg tgatcatgga cgtcaagatg cccgtgatgg acgggctcgc
     5401 ggccgccgac gagatcggca agcagcgcct gtgccccgtc atcatgctga ccgccttctc
     5461 ccagaccgaa ctggtcgaac gggcccgcga cgccggagtg atggcctata tcgtcaagcc
     5521 cttcactgcc gccgacctgg cgccggcgat caatatcgcc ctctcgcgct ggagcgaact
     5581 gaaggaactc gagaaggagg tcgccgacct cggcgaggcc ctcgagacac gcaaggccgt
     5641 cgaacgggcc aagggcgtgc tgatgaagaa gctcaagctg tccgaggccg aagccttccg
     5701 ctggatccag aagaccgcga tggaccggcg catggggatg aaggacgtcg ccgaggccgt
     5761 catcgccggg atcgacaagg cctgacccgg ccggtcctgc tgcaactcgt cacaaagtca
     5821 catccgactc agacgtccca ggcgttcccg caggtcaggg acctcgtcgt ggcgtgcgga
     5881 acaccgggtt tcgcaacccg acccccaggc cttagagtgc ccgttaccgg ctggtcaacg
     5941 cgcccccacg cgctgctctc ccggacctgt ccgaccgctg tattccacgc ctcatgagga
     6001 gcccccgtgg cacgtcccca cctcaatgtg accaaggccc tccccggcga tgccggtgat
     6061 ctgtcggcgt tgtggatcga atgggcgctc tgctccggga tgagccacga cctggcggcg
     6121 cgggccggcg atgtcgcgcg gatcaccgaa gccctcgagc gccccgtaac gaccgtgctg
     6181 ctggcgcgtc ttgaccaccg gcccgtcggc ttcgccgtcg tcaacacccg caatcacggc
     6241 ctgctcgacc cggccggcct cgcgatcgac gagctctatg tcatccccga aggtcgccgc
     6301 cagggcgtcg cggccggcct cctcggcgcg gtggcgacca tggccgagag cgagggccac
     6361 gacctggtct tcgccaatgt cgccgccacc gacaagctga gcaatcgcta tctcgcccgc
     6421 ctcggattcg gcaccaccat cacccgccgc gtggtgccga cggcggtgtt gcgccgcaag
     6481 ctgtccggcg ccgacaccgc gtcccaggag atcctgctcg gcaaacggcg caccctccgg
     6541 gcgcggtccc gcggcgccgt gggctcggcg gcaccggcgc gggcaatctc gttgcgccgc
     6601 accgcaatct agctctcact cggccttcgc acccggcggg agatcccgca gcaggcaggt
     6661 gatccgcgac gtgcagcacc ggcgcccctg atcgtcggtg atgaccacct catacgacac
     6721 catcgtcttg ccggcggaga gcacggtggc gactcccgtg acgatgcccg agcgcgcggc
     6781 ccggtggtgg gtggcgttga tatcgatccc gaccgctgcg cgcgccggac cggcgtgcat
     6841 ggccgagccg agcgagccga gggtctcggc caggaccaca ctcgcgcccc cgtgcagcag
     6901 gccatacggc tgctggttgc cggccaccgg catcgtgccc accacccggt ccttgctcaa
     6961 ctcggtgatc tcgatgccca tccgatcggc gagggtcccc ttcgacatgg cgttgacggc
     7021 cgcgaggacg tccgcggagg aggagaagat ggcgttgtcg gtcatgacgc ataggctgcc
     7081 atgcgtgagt cgcctactcc tcctcgacgg tcattcgctg gcctatcgcg ccttctacgc
     7141 gctgccggcc gagaacttct cgaccaagac cggccagcac accaacgccg tctacgggtt
     7201 cacctcgatg ctcatcaatg tgctgcgcga cgaggacccg acacacatcg ccgtggcctt
     7261 cgaccactcc cggcagacct tccggatgga ggagtatgcc gaatacaagg ccggccggtc
     7321 cgcaacgccg agcgagttct ccggccagct gcccctgctg cgcgaggtcc tcgacgccct
     7381 ccagatccgc tggctagagg tgccgggttt cgaggccgac gacatcatcg ccacgctgac
     7441 gacgcgcgcc gccgagcagg acatggaggt cgtcatctgc tccggcgacc gagacgcctt
     7501 ccagctggtc accgacacgg tcaccctcct gtatcccgtc cgcggggtct ccgaggtgtg
     7561 gcggatgacg ccggccaagg tggaggagaa atatggcgtc ccgccgcagc gctacggcga
     7621 tctcgcggcc ctggtcggtg agtcgagcga caatctgccg ggggtgccgg gagtcggtcc
     7681 caagacggcc gcgaagtgga ttcagcagta tgacggcctg tccggcatcg tcgcccacgt
     7741 cgaccagatc ggtggcaagg tcggtgccgc gctgcgcgac aacctcgagc aggtgttgcg
     7801 caaccggcgg ctcaaccagc tcgtccgcga tctcgacacc gggctcaccg tcgatgacct
     7861 ccagcggcgg atgtgggacc gcgagcgggt ccacgaggtc ttcgacgccc tcgagttccg
     7921 cgtgctgcgc gagcggctgt tcaccacctt cgacacgacc gcggtcgagg ccgagggcgg
     7981 cttcgacgtc gagacccgct cgctggcagg cggcgacgtg ggggagtggc tcgccggccc
     8041 ggggcgcacg ggcgagatcg gcgtccacgt cgtcggctcg tgggcccgcg gtgacgggga
     8101 cgtcggggcg ctggcgctgg ccggctccga gggcccggcc gtcttcatcg acgtcaccca
     8161 actcgatccc gacggcgaag ccgccctcgg gcattggctg gccgaccccg aggtgcccaa
     8221 attcgttcac gatgccaaag ggccgataca cgctctgcac gcgcgcgggt gggagctggg
     8281 cgggctggcg ctggacacgg cgctggccgc ctacctcgtc aaacccgacc agcgcgactt
     8341 cgacctggcc gatctggtcg tgcggatgct cggtcgtgac ctgcgggccg aaaccggtgc
     8401 cgcagagggg atgctcgact tcggcgccga tgatcaggcc gagatcgccg acgccggcgt
     8461 ccgggcgcgc gcggtcctcg acctcggcca cactctcgcg gagcaggtcg agaaggccgg
     8521 gggcacccgg ctgttgcgtg aggtcgagct gcctctggtc ggcgtgctcg ccgagatgga
     8581 gcagacgggc atcgccgtgg acctgaccca cctcgaagcc ctcgagaagg acttcgccga
     8641 cgcggtcgag cgggcccagg aggatgccta tgacgcgatc ggcggcgagc agatcaacct
     8701 cggctctccg aagcagctgc aggtcgtcct cttcgacacc ctcgccatgc ccaagaccaa
     8761 gcgcaccaag acgggatgga ccaccgacgc ggaggcgctg gctgatctct acaccaagac
     8821 ggagcacccg ttcctcgagg cgctgctgcg ccaccgcgac gcgacccggc tgcgggtcac
     8881 ggtcgagggg ctgctgaagt cggtcagcga cgacggacgc atccacacga catacgagca
     8941 gaccattgcg gcgaccgggc gcctgtcctc caaggacccc aacctgcaga acatcccgat
     9001 ccgcaccgac gagggccggc gcatccgcga gagcttcgtc gtcggcccgg gctacgagtc
     9061 gctgatgtcg gtcgactaca gccagatcga gatgcggatc atggcccacc tgtccggcga
     9121 cgcgggtctg atcgaggcct tccggtcggg ggaggatctt caccggttcg tcggctcacg
     9181 cgtcttcgcc gtggcgccgc aggacgtgac gccggcgatg cgcagcaaga tcaaagcgat
     9241 gtcctacggc ctcgcctacg ggctgtccgc gttcgggctc tcccggcagc tgacgatccc
     9301 acgcgaggag gccagcgcgc tgatggagga gtacttcgcc cgcttcggtg gcgtccgcga
     9361 ctacctgcgc gatgtcgtag ccgacgagcg cgcgaccggc tacaccgaga cgatgctcgg
     9421 ccggcgccgc tacctgcccg acctgaccag cgacaaccgg cagcggcgcg agatggccga
     9481 gcggatggca ctcaacgcac ccatccaagg cagcgccgcc gacatcatga aggtcgcgat
     9541 gatcaacgcc cacaacgcat tgcgcgacgc cggtctgaac tcgcgcctgc tgctccaggt
     9601 ccacgacgaa ctcgtcgtcg aggtcgcccc cggcgaggcc gagcaggtcg aagcgctcgt
     9661 gcggcgcgaa atgggttccg ccgcaggcga gatcgacgtc cccctcgacg tcagtgtcgg
     9721 gatcggccgc tcctggcacg acgccggcca ctaggccacc ccacgacgcg tcctcgggcg
     9781 aggtcctaga gtcggtgcat gccagcagac caccccacga tcttggccac ctccggcggc
     9841 taccgattcg gcacccgcac ccggatggag ttcaaccacc tcgtccacca cgcggtggag
     9901 ttggcggggg tcagtggcca cgcaccccgg gtgtgcctgg tgggtacggc ctccggggac
     9961 cagcgctggt tcgccgccga gatggacgag gcggcccggg tcgcgggctt cgacctgacg
    10021 cacctgcact tgttcccgat gccgaacgtc gaggacatcg agggccacct gatggcccag
    10081 gacgtcgtgt gggtcaacgg cgggtcggtc gccaatctcc ttgcggtgtg ggatgttcac
    10141 gacttgcgtc cggtcttccg tcgcgcctgg gaggccggtg tcgtgctcag cggcgtctcg
    10201 gccggttcga tctgctggta tgacggcggc tgtaccgact cctacggccc cgacctgcgc
    10261 gcggtcacca acgggctggg gttgctgcca tacggcaatg gcgtgcacta cgactccgag
    10321 gagcagcgcc ggcccaccgt ccaccggctc gtcggcgacg gcaccctggc attgacccac
    10381 tgcaccgacg acggcgtcgg catcgtttac cacggcaccg acctggtcga ggcggtcac
//
LOCUS       DAOPJG010000118        10411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6723_length_10411_cov_7.528100, whole genome shotgun sequence.
ACCESSION   DAOPJG010000118 DAOPJG010000000
VERSION     DAOPJG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6723_length_10411_cov_7.528100"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PKU75_11045"
     CDS             complement(<1..1137)
                     /locus_tag="PKU75_11045"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPF81460.1"
                     /translation="MEFRSAYAHGFARVAAVTLPVALAQPAKNAEAILEQARVCHEES
                     VALAVFPELGLSGYSLEDLFLQDPVLDAVEEALYAVVDGSRDLLPVLVIGAPLRKGNR
                     IFNCAVIIHRGTILGVAPKSYLPTYREFYERRHFAPGDDQRGQSIRLRETEIPFGPDL
                     IFRATDVRGLTVHVEVCEDMWVPIPPSAEASLAGAAVLVNISGSPITVAKSYDRKLLC
                     ESSSSRCNAAYLYAAAGEGESSTDLSWDGQTMIYEMGALLAETERFPAGARHSVADVD
                     LDLLRQERMRQGTFDDNRRTYGDRTAYRTIDFTLDPPHTDIGLLRKVDRFPFVPDEEE
                     RLALDCYEAYNIQVSGLEQRLRAIGQPKIVIGVSGGLDSTHALIV"
     gene            complement(1225..2613)
                     /locus_tag="PKU75_11050"
     CDS             complement(1225..2613)
                     /locus_tag="PKU75_11050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HPF81461.1"
                     /translation="MSRTQRASWRTPSDVEQKPDPRHTAEPAPGGTGSPDQTDGTGSP
                     DQTGNTDWSVSRRLLPSFFAAVAALLWAGPYLHLQPWIGFHLTDIPTYQRSADATAAG
                     RLPYRDVDLEYPPGAAVIFWLARFLPGQGYVEKFQWFMLLFWALATAGAVVAAGQLRL
                     PVRRQVAVGTVMAITPLVLGTIVRTRYDGVVTAVVAWMIVAALAERWRWMWGLLAVAI
                     ALKLMPALLAPALVAWQAHRAGWASARRGAAGGIAATVALFAPAALLAPRGLWDMVMY
                     HLQRPPQIESTASSYVLLWHATAGVPVTIESAFGSQGPTGQTAEVLATATTLLAFLAI
                     VAVSVRYARHLGALVRGLDAAYLLATLAATIVFLAVGSKVLSPQYLLWIVPASLLLPG
                     RRGLTAFVMTPVLMLVTHLYFPFRYWNLVGLETLPIWILFVRNLLLIALACVCWSVSP
                     AAPTPRAPALAD"
     gene            complement(2610..3107)
                     /locus_tag="PKU75_11055"
     CDS             complement(2610..3107)
                     /locus_tag="PKU75_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81462.1"
                     /translation="MKLRARLGLACTTATAAVMTYAAVYASAAPVPQGFGRVTVTITP
                     TILVSTNSEGDPMTVGTSTPTPEGAAESGFTALTWIPLAVLVLLAVAAFAWWLASRNN
                     RRDHLAAGTRRTSGATAAAQFGGSEEPDSGRPDSDKPDFGKPDLDKPDGEEPDHGGPE
                     RRIQR"
     gene            complement(3214..4011)
                     /locus_tag="PKU75_11060"
     CDS             complement(3214..4011)
                     /locus_tag="PKU75_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPF81463.1"
                     /translation="MTEHVTTVVADQIAFVRLDRPEKMNALSLPMLKELVATAHRLRG
                     DTGLRGVLISGEGSAFCAGIDTSVLAGGLKSFGGPFLPRPWLGTNVFQEACWAWRRIP
                     VPVIAVVHGHCYGAGLQLALGADFRVSTPDAKWSVLEAKWGLIPDMSGIQALKQLVGM
                     DVAKQLTMTARTLTGEQARELGLVTQVAADPHIAASELLEEILVRSPDSVAATKRIFE
                     DTWGAGARRTFARERIEQARLLMARNTRIVRAAASSKTTPEYAPRGR"
     gene            4117..4359
                     /locus_tag="PKU75_11065"
     CDS             4117..4359
                     /locus_tag="PKU75_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81464.1"
                     /translation="MPLTVVELNVLGGSASPAAVDEGTRDVSLVVLTELDTSTAAALD
                     ALGWDERFPHRVGRADPGGWSRQPTSPAPDEPKKIR"
     gene            complement(4407..5021)
                     /locus_tag="PKU75_11070"
     CDS             complement(4407..5021)
                     /locus_tag="PKU75_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPF81465.1"
                     /translation="MTLSMLAGALPAARTSIWSDMWNFQIPATEKILRTIGVYVAILV
                     IVRLAGKRLMAQMNSLDLVVVLLLSNVVQNAIIGDDNSLTGGILGAIVLVLVNAGMDR
                     AAQHWPAFDRLTSGSPTPVVRDGEIVEDSLDRLGMTAAELDSALRHQGADSIGEVAVA
                     TIDPGGSITVDLKEAEQALSRGEFEAAMTQLHRRLDALTSPPPR"
     gene            complement(5123..5470)
                     /locus_tag="PKU75_11075"
     CDS             complement(5123..5470)
                     /locus_tag="PKU75_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF81466.1"
                     /translation="MPRSPRGNTRDQLTAALIELVAGEGMDRASVREVATAAGVGIGT
                     VQYHFPTRRDLLTGAFEAVVHGVRDRLALPAARADADPAATVVAILRELVPLDAPRRR
                     EAAVMVAFAAGGP"
     gene            complement(5500..5982)
                     /locus_tag="PKU75_11080"
     CDS             complement(5500..5982)
                     /locus_tag="PKU75_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81467.1"
                     /translation="MRQHGRDTVPPAAPPALPHVHPHPRIGLQVADISHAHPVLGDDP
                     DHLAVAAVPDGHLAHSAGAAPGRLEQRDAERVDAEPVEAADERVDHPALQRRDEARLD
                     AWCAPGERDIREQEPGLDERAGQAGPRGRVEREDAPHQGEAREGDQREAVRSSRHTVV
                     "
     gene            complement(6233..7486)
                     /locus_tag="PKU75_11085"
     CDS             complement(6233..7486)
                     /locus_tag="PKU75_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPF81468.1"
                     /translation="MADAPLFTAERAAEFAAEADRLLDFTAGAKTTGGFGWLGDHGEV
                     VERDLELWITCRMTHVCALGAAIGKPGFLELTEHGIRSIADNFHDERHDGWYAAISPD
                     GSRPTDATKAAYAHSFVILAGSSGQAVGAAGAADLLTHALAVHDRFFWDESQGMTRES
                     FSGDWRTEEDYRGINANMHTVEAYLAAGDVTGDQLWHERAGRIVTRAIDQLARANSWR
                     IPEHFTADFTPLPDYNKDQPAHPFRPYGATPGHALEWARLTLAAAQTLGGPAWDWAYD
                     AASALVAGAITDGWAPDGADGFVYTTDWDGTPSSHARMFWVLTEAIGAAWALHHVDGS
                     AQWRPLVEKWWAYAKAYLLDLDRGAWHSELDATNAPYAGTWVGKPDTYHAIQAMLLPR
                     LPLTPSFTTALAARGAPEAGGTGSA"
     gene            7636..8988
                     /locus_tag="PKU75_11090"
     CDS             7636..8988
                     /locus_tag="PKU75_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81469.1"
                     /translation="MGTTPGTPQYARRALLVAAVTLGALVASAAFRSSTGVLMEPIES
                     EFGWSRATTAGAVSLNLVLFGCIAPFAAALLERYGPRRVVTIALLVVGAASALTTVMT
                     SPWQLWLLWGLVIGTATGSTALVLGAIVANRWFVARRGLVTGVFSGANATGQLLFLPA
                     IAATATNYGWRWSAAIVAVLAALTAVLVWLFMRDRPQDIGLIPFGAAPGSPEAAPAPP
                     PAVSPVRAALSVLRTSSRTWVFWALMLTFFVCGWSTNGLVQTHFIPAAHDHGMPATTA
                     AGLLALVGIFDLVGTIASGWLTDRVDPRLLLMAYYALRGGSLFTVHSLLAPGVEPGMW
                     VFIIFYGLDWVATVPPTVALCRSHFGLAHSGIVFGWTYAAHMVGAGLGASFAGWIRTA
                     DGTYRTAWMSAGVLCVIAAFLCLTIPRSPAAPVPTDHLPTPPTRYPDDVDDPEELTFT
                     "
     gene            complement(9055..9978)
                     /locus_tag="PKU75_11095"
     CDS             complement(9055..9978)
                     /locus_tag="PKU75_11095"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPF81470.1"
                     /translation="MLDATRLATFRSVVANGTVQAAADQLRLTPSAVSQQVAALQKET
                     GLTLLERVGRGVRPTPAGMTLAVASGDALRSLNRLTALVRDLREGKTGHLVIGHFASA
                     GQAWMPDLVARISREFPDIMVQLVLTDIPGENLPTKPDIDVRIALAGEGIPVGMQRIA
                     LLEDPFVMCVHKSHPLARKRTPVPLADFAEDRFVANDVTGGSVHAIQSVAYAAAGFSP
                     RTIVEANDHRVALAFVDRGIGISMLPGLAAGDLPPNVRIVQLADPVPHREIYALVRTA
                     IAATPAARRVVELLHKHAAASQSGLASPAHR"
     gene            10060..10194
                     /locus_tag="PKU75_11100"
     CDS             10060..10194
                     /locus_tag="PKU75_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81471.1"
                     /translation="MDRPIDRDYARMLTELRTVRQDWHTELPQPKKRSIVYRLTHRAH
                     "
BASE COUNT         1496 a         3675 c         3677 g         1563 t
ORIGIN      
        1 gacgatgagc gcgtgggtgg agtcgaggcc gccggagacg ccgatgacga tcttcggctg
       61 gccgatcgcc cgcaggcgct gctcgagccc ggagacctgg atgttgtagg cctcgtagca
      121 gtcgagcgcg agccgctcct cctcgtcggg gacgaacggg aagcggtcga ccttgcgcag
      181 caggccgatg tcggtatgcg gcgggtcgag ggtgaagtcg atcgtgcggt aggccgtccg
      241 gtcaccgtag gtgcgccggt tgtcgtcgaa cgtgccctgc cgcatccgct cctggcgcag
      301 caggtcgagg tcgacgtcgg cgacggagtg gcgggccccg gcggggaagc gctcggtctc
      361 ggcgagcagc gcccccatct cgtagatcat ggtctgcccg tcccaggaca agtcggtgct
      421 cgactcgcct tctcccgcag cggcatatag gtatgccgcg ttgcagcggg aggaggagga
      481 ctcgcacaac agcttgcggt cgtaggactt ggcgactgtg atcgggctgc cggagatgtt
      541 caccaggacc gccgccccgg ccagcgaggc ctcggcgctc ggcgggatcg gcacccacat
      601 gtcctcgcac acctcgacgt gcacggtgag cccgcgcacg tccgtggcac ggaagatcag
      661 gtcgggtccg aaggggatct cggtttctcg caaccggatc gactgcccgc gctggtcgtc
      721 cccaggcgcg aagtgccggc gctcgtagaa ctcgcgatag gtcggcaggt acgacttggg
      781 cgccacgccg aggatcgtgc cgcggtggat gatcaccgcg cagttgaaga tccggttccc
      841 tttgcgcagc ggcgccccga tgacgagcac cggcagcagg tcccgggagc cgtcgaccac
      901 ggcatacagc gcctcctcga ccgcgtcgag caccgggtcc tgcaggaaca ggtcctcgag
      961 cgaatagccg gacagcccca gctccgggaa gaccgccagg gccaccgact cctcgtggca
     1021 gacccgagcc tgttcgagga tcgcctcggc gttcttggcc ggctgggcga gcgcgacggg
     1081 cagggtgacg gcggcgaccc gggcgaaccc gtgcgcgtag gccgaacgga actccatgcg
     1141 gccgatccta tgggcagcgt cggggccgac ccggcgggcc gccggtgaca tctcgacggg
     1201 caggaatcga catctcgacg ggcgtcagtc ggcgagcgcc ggggcgcgtg gggtgggggc
     1261 ggccgggctg accgaccagc acacgcacgc cagggcgatc agcagcaggt tgcgcacgaa
     1321 gaggatccag atcggcagcg tctcgaggcc gacgaggttc cagtagcgga acgggaagta
     1381 caggtgcgtg acgagcatca ggacaggggt catcacgaac gccgtcagcc cgcgccggcc
     1441 cggcagcagc aggctggccg ggacgatcca cagcaggtat tgcggtgaga ggactttcga
     1501 gccgacggcg aggaagacga tggtcgccgc gagtgtcgcc aggaggtatg ccgcgtcgag
     1561 accgcgcacg agcgccccga ggtggcgggc gtagcggacg gagacggcga cgatggcgag
     1621 gaaggccagc agggtggtgg cggtggcgag cacctccgcc gtctgcccgg tcggtccctg
     1681 gctgccgaag gccgactcga tcgtgaccgg cacccctgcc gtcgcgtgcc acagcaacac
     1741 atacgagctg gccgtggact cgatctgcgg gggccgttgg aggtggtaca tcaccatgtc
     1801 ccacagtccg cggggcgcga gcagggcagc gggggcgaag agcgcgaccg tggccgcgat
     1861 cccgccggct gccccccgcc gggccgaggc ccagccggcg cggtgggcct gccaggcaac
     1921 cagggccggc gcgagcagcg ccggcatcag cttcagcgcg atcgcgacgg cgagcagccc
     1981 ccacatccag cgccagcgtt cggcgagcgc cgcgacgatc atccaggcga cgacggcggt
     2041 gaccacgccg tcataccggg tgcgcacgat cgtgccgagc acgagcggcg tgatcgccat
     2101 gacggtcccg acggcgacct gccggcgcac cggcagccgc agctgcccgg cggcgacgac
     2161 ggcccccgcg gtggccagcg cccagaagag cagcatgaac cactggaact tctcgacata
     2221 accctggccg ggcaggaacc gggccagcca gaagatgacc gccgcgcccg gggggtactc
     2281 gaggtcgaca tcccggtagg gcagccggcc tgccgccgtc gcgtccgccg accgctgata
     2341 ggtcgggatg tcggtcaggt ggaacccgat ccacggctgc aggtgcaggt agggcccggc
     2401 ccacagcagc gcggcgacgg ctgcgaagaa ggacggcagc agccgccggg agacgctcca
     2461 gtcggtgttg ccggtctggt cggggctgcc ggtgccatct gtctggtcag ggctgccggt
     2521 gccgccgggt gccggttcgg cggtatgccg cggatccggc ttctgctcga cgtcgctcgg
     2581 ggtgcgccac gaggcgcgct gggtgcggct caccgctgga tgcggcgctc ggggccgccg
     2641 tggtcgggct cttcgccgtc cggcttgtcg aggtccggct tcccgaagtc aggcttgtcg
     2701 gagtcgggcc tgccggagtc gggctcctcg cttccgccga actgcgccgc cgccgtcgcc
     2761 ccactcgtcc ggcgggtgcc ggcggccagg tggtcgcgcc ggttgttgcg ggatgcgagc
     2821 caccaggcga aggccgccac cgccagcagc accagcacgg ccagcgggat ccacgtcagc
     2881 gcagtgaacc ccgactccgc ggcgccctcc ggcgtcggcg tgctcgtgcc cacggtcatc
     2941 gggtcgccct cgctgttggt gctgaccagg atggtcgggg tgatggtcac ggtgacccgc
     3001 ccgaagccct gggggacagg tgcggcgctg gcatacaccg cggcatacgt catcaccgcc
     3061 gcggtcgccg tcgtgcaggc caggccgagg cgcgctcgca gcttcactct tggcttctct
     3121 tctcactcga gccggggcgg atgaccgccc cgctcagggt accggtagca ccggaccgcc
     3181 ccgtgcagcc agaccccgtc cgcgggccag gtttcagcgc ccgcgcggcg catactccgg
     3241 ggtcgtcttc gaactcgccg ccgcccggac gatgcgggtg ttgcgggcca tcagcaaccg
     3301 ggcctgctcg atgcgttcgc gggcgaaggt gcggcgcgcc ccggcgcccc acgtgtcctc
     3361 gaagatccgc tttgtcgccg ccaccgaatc gggcgagcgc acgaggatct cctcgagcaa
     3421 ctcactcgcg gcgatgtggg gatcggcggc gacctgcgtg accaggccta gctcacgcgc
     3481 ctgctcgccg gtcagcgtcc gggccgtcat cgtcagctgc ttggccacgt ccataccgac
     3541 aagctgtttg agcgcctgga tcccgctcat gtccgggatc agaccccact tcgcctccag
     3601 cacagaccat ttcgcgtccg gtgtggagac ccggaagtcg gcgccgagcg ccagctgcag
     3661 ccccgcgccg tagcagtggc cgtgcacgac cgcgatgacc ggcaccggga tccgccgcca
     3721 cgcccagcag gcctcctgaa agacgttggt gcccaaccac ggtcgcggca ggaacggccc
     3781 gccgaagctc ttcagtccgc ccgccagcac gctggtgtcg atgccggcgc agaaggccga
     3841 cccctctcct gagatcagga ccccacgcag gccggtatcg ccgcgcagcc gatgcgccgt
     3901 ggccaccagc tccttgagca tcggcaggct gagggcgttc atcttctccg gccggtcgag
     3961 gcggacgaag gcgatctggt cggccaccac ggtggtcacg tgctcggtca tgggtcctca
     4021 gcgtaggcac cggccgggcc cgcgtcaccg gggccgcgtt gtgcgcacgc ccacacccgc
     4081 ggcttctatt cgtgggccgg tgacccgccc gccggcgtgc cgctcaccgt ggtcgaactc
     4141 aacgtgctgg gcgggtcggc gagcccggcc gccgtggatg aggggacgcg ggacgtctcg
     4201 ctggtcgtcc tgaccgaact cgacacgagc accgcggccg cgctcgacgc gctgggctgg
     4261 gacgagcggt tcccccatag ggtcgggcgg gccgacccgg gcggctggag tcggcagccg
     4321 acctcgccgg cgccggatga gcccaagaag attaggtaag acgctttggc tgttataacc
     4381 gccagagtgt cttacctacc ggcgggtcat ctcgggggcg ggctcgtcag cgcgtcgagt
     4441 cgccggtgca actgcgtcat cgccgcctcg aactcgccgc ggctgagggc ctgctcggcc
     4501 tccttgaggt cgacggtgat cgacccgccc gggtcgatgg tcgccactgc cacctcgccg
     4561 atcgagtccg cgccctgatg gcgcagtgcc gagtcgagtt cggccgccgt catgccgagc
     4621 cggtcgaggc tgtcctcgac gatctcgccg tctcggacga ccggggtcgg tgagccggat
     4681 gtcagccggt cgaaggccgg ccagtgctgc gcggcccggt ccatcccggc gttgacgagg
     4741 acgagcacga tcgcaccgag gatgccgccg gtgagcgagt tgtcgtcgcc gatgatggcg
     4801 ttctgcacga cattgctcag cagcaacacc acgacgaggt cgagactgtt catctgcgcc
     4861 atcaaccgct tcccggcgag ccgcacgatg accaggatcg cgacatacac cccgatggtc
     4921 cgcaggatct tttccgtcgc cgggatctgg aagttccaca tgtcactcca gatcgaggtc
     4981 cgcgctgcgg gcagggcacc cgccaacatg ctcagcgtca tgcagcgacg ctacgcccgc
     5041 gggcgggcca gcagctggga cagcgtgtcg aggatggcct cgactcgcgc cggggtcccc
     5101 ctgcagcgcc gccagttccg gctcatggac cgcctgcggc gaaggcgacc atgaccgcag
     5161 cctcccgccg ccgtggcgca tccaagggca ccagttcgcg caggatcgcg acgacggttg
     5221 cggccgggtc ggcgtcggcg cgggcggcgg gaagcgccaa ccggtcccgg accccgtgca
     5281 cgacagcctc gaaggcaccg gtgagcaggt ccctgcgtgt cgggaagtga tattgcacgg
     5341 tcccgatccc caccccggcc gcggttgcca cctcgcgcac cgaggcccgg tccatgccct
     5401 cgccggcgac gagctcgatg agggcggcgg tcagctgatc gcgggtgttt ccccttggcg
     5461 agcgcggcat atctggcgtc atacagtcgt atgaatccct tatacgaccg tatgacgcga
     5521 cgatcggacc gcctcgcgct ggtcaccctc gcgggcctcg ccctggtgtg gggcatcttc
     5581 gcgctcgacc cggccgcgtg gcccggcctg tccggcgcgc tcgtccagtc cgggctcctg
     5641 ctcgcgaatg tcgcgctcac ccggcgcgca ccacgcgtca aggcgcgcct cgtcgcggcg
     5701 ctgcagcgct gggtggtcaa cccgctcgtc cgcggcctct accggctcgg cgtcaacccg
     5761 ctcggcatcg cgctgctcga gacgaccggg cgccgctccg gccgagtgcg ccaggtgccc
     5821 gtcggggacg gccgcgacgg cgaggtggtc tgggtcgtcg ccgagcacgg gatgcgcgtg
     5881 ggatatgtcc gcaacctgca ggccgatccg cgggtgcgga tgcacgtgcg ggagggctgg
     5941 cggcgccgct ggcgggacgg tgtcgcgacc gtgctgccgc acgacgatct ctttgcgcgg
     6001 caacgttcct cgtccgtggc cacccgctgc gcgccctcaa cgccgcgagc gtgcgcctgc
     6061 tcggcgcgga accggtcacg gtccggatcg acctgctgtc cgcggactct ccgccagttc
     6121 gcgatcaggc ctggcgggcc aacaacagca cagcgatggc gcccggcgag ggcacctttg
     6181 tcgcgctggt ctcgtgcgcc acgcagagaa ggtgacggag cagacgccgg gcctatgccg
     6241 acccggtgcc ccccgcctcg ggcgcgcccc gcgccgcgag cgcggtggtg aaggaggggg
     6301 tcagcggcag ccgcggcagc aacatcgcct ggatggcgtg ataagtgtcg ggcttgccca
     6361 cccaggtccc ggcgtacggg gcattggtcg cgtccaactc cgagtgccag gcgccgcggt
     6421 cgagatcgag gaggtaggcc ttggcatagg cccaccactt ctcgacgagc ggccgccact
     6481 gcgccgaccc gtcgacatga tgtagcgccc acgctgcgcc gatcgcttcg gtgagcaccc
     6541 agaacatgcg cgcgtgcgag ctcggggtgc cgtcccagtc ggtcgtgtag acgaagccgt
     6601 cggcgccgtc cggtgcccag ccgtcggtga tggcgccggc gacgagggcg cttgccgcgt
     6661 catacgccca atcccacgcc ggcccgccga gggtctgcgc cgccgccagc gtcaggcgcg
     6721 cccactcgag ggcgtggccg ggggtggcgc cgtacggccg gaacgggtgc gccggctggt
     6781 ccttgttgta gtccggcagc ggggtgaagt cggcggtgaa gtgctcgggg atgcgccagc
     6841 tgttcgcgcg cgcgagctgg tcgatggcgc gggtcacgat ccgcccggct cgctcgtgcc
     6901 agagctggtc accggtcaca tcgcccgcgg cgagataggc ctccaccgtg tgcatattgg
     6961 cgttgatgcc gcggtagtcc tcctccgtgc gccagtcgcc ggagaacgat tcgcgggtca
     7021 tgccctgtga ctcgtcccag aagaagcggt cgtgcaccgc gagcgcgtgg gtgagcagat
     7081 cagcggcacc cgccgctccc accgcctgcc cgctggagcc ggcgaggatc acgaacgagt
     7141 gggcgtaggc cgccttcgtc gcgtcggtgg gccggctccc gtcgggcgag atcgcggcat
     7201 accacccgtc gtggcgctcg tcgtggaagt tgtcggcgat gcttcgtatg ccgtgctcgg
     7261 tcaactcgag gaagcccggc ttgccgatgg cggcgccgag cgcacagaca tgggtcatcc
     7321 ggcaggtgat ccacaactcg aggtcgcgct cgacgacctc accgtggtcg ccgagccagc
     7381 cgaatccccc cgtcgtcttc gctcccgctg tgaagtcgag gaggcggtcg gcctccgcgg
     7441 cgaattcggc ggcacgttcg gcggtgaaca gcggagcatc ggccatgccc cgattcgacc
     7501 acacccgggt ccggcgcggc gaggccggcc aaggaaactc tgctaacaga ggttggtcga
     7561 atcagcagac ctagggaggt gtgcggattt gaccaacctc tgctgggatg gcggcgagcg
     7621 tgccatcatg cgcgaatggg gaccacaccg ggcacgccgc agtatgcccg tcgggccctg
     7681 ctggtcgcgg ccgtcaccct cggcgcgctg gtcgcctccg ccgccttccg ctcctccacc
     7741 ggtgtcctca tggagccgat cgagagcgag ttcgggtgga gccgggcgac gacggccggg
     7801 gcggtcagcc tcaacctcgt cctcttcggg tgcatcgcgc ccttcgccgc ggcgctgctc
     7861 gagcgctacg gcccgcgccg ggtggtgacg atcgccctgc tcgtcgtcgg cgccgcgtcc
     7921 gcgctgacga cggtcatgac gagtccctgg cagttgtggc tgctgtgggg cctggtgatc
     7981 ggcaccgcca ccgggtcgac ggcgctcgtg ctcggcgcca tcgtcgccaa ccggtggttc
     8041 gtcgcccgcc gcggcctggt gacgggcgtc ttctccggcg ccaacgcgac cggccagctg
     8101 ctcttcctgc cggcgatcgc ggcgacggcg acgaactacg gctggcgctg gtcggccgcg
     8161 atcgtcgcgg tcctggccgc gctcaccgcc gtcctggtgt ggctgttcat gcgcgaccgc
     8221 ccccaggaca tcggactgat ccccttcggt gccgcccccg ggtcgcccga ggccgcgccg
     8281 gccccgccgc cggccgtctc ccccgtccgc gccgcgctgt cggtgctgcg cacgtcgagc
     8341 cgcacctggg tcttctgggc gttgatgctg accttcttcg tgtgcggctg gtcgaccaac
     8401 ggcctggtcc agacccactt catcccggcc gcgcacgatc acggtatgcc ggccaccacc
     8461 gccgccggtc tgctcgccct cgtggggatc ttcgacctcg tcggcaccat cgcctccggc
     8521 tggctgaccg accgtgtcga tccgcgcctg ctcctgatgg cctactacgc gctgcgcggc
     8581 gggtcgctct tcaccgttca ctccctcctc gcgccgggcg tcgaaccggg aatgtgggtc
     8641 ttcatcatct tctacgggct cgactgggtg gccaccgtcc cgccgacggt tgcgttgtgc
     8701 cgcagccact tcgggcttgc tcactccggc atcgtcttcg gctggacgta tgccgcgcac
     8761 atggtcggcg ccggcctcgg cgcctccttc gccggctgga tccgcaccgc cgacggcacc
     8821 taccgcacgg cctggatgag cgccggggtc ttgtgcgtga tcgccgcctt cctttgcctg
     8881 accatccccc ggtcacccgc ggcgcccgtc cccacggacc acctgcccac cccacccacc
     8941 cgctatcccg acgacgtcga tgaccccgag gaactcacct tcacctgacc ctcccccgtc
     9001 gagatgtcga tttggccgcg tcgagatgtc gattcgaggg cgtcgagagc gcaatcagcg
     9061 gtgcgcaggg gaagccaggc cggactggga agccgcggca tgcttgtgga ggagttcgac
     9121 gacgcgccgc gcggccggtg tcgcggcgat cgccgtccgg acgagggcat agatctcccg
     9181 gtgtggcacc ggatcggcca gctggacgat ccgcacgttc gggggcaggt cgccggccgc
     9241 gagaccgggg agcatcgaga tgccgatgcc gcggtcgacg aacgcgagcg cgacccggtg
     9301 gtcgttggcc tcgacgatgg tacgcgggct gaaaccggcc gcagcgtagg ccacggactg
     9361 gatcgcgtga accgacccgc cggtcacgtc attggcgacg aaacggtcct cggcgaagtc
     9421 cgccaagggc acgggggtgc gcttgcgggc gagcggatgg ctcttgtgca cgcacatgac
     9481 gaatggatcc tcgaggaggg caatgcgctg catgccgaca ggaatcccct cgcccgccaa
     9541 ggcgattcgc acgtcgatgt ccggcttcgt agggaggttc tccccgggta tgtccgtcag
     9601 gacgagttgc accatgatgt ccgggaactc gcggctgatc cgggcgacga ggtcgggcat
     9661 ccacgcctgg ccggccgacg caaagtggcc gatgacgagg tggccggtct tgccctcccg
     9721 gaggtcgcgc accagcgccg tgagccggtt gagactgcgg agagcatcgc cgctggccac
     9781 cgccagtgtc atcccggcgg gcgtcggccg aactccgcgc cccacccgct cgaggagcgt
     9841 caagccggtc tccttctgca gtgctgcgac ctgctgactg accgccgacg gggtgagccg
     9901 caactggtcg gcggcggcct gcaccgtccc attggcgacg accgaacgaa aggtggcaag
     9961 tcgggttgcg tcgagcacgg gtcgataata cagattctct taatggatga tcagtaagtc
    10021 tcgcttgtgc taaatcatta aacgctgcac catagagata tggatcgccc gattgaccgt
    10081 gactacgccc gcatgttgac cgaactccgc accgtccgcc aggactggca caccgaactg
    10141 ccgcagccca agaagcgcag catcgtctac cgtctcaccc accgcgcgca ctgaaacacc
    10201 ctctgcacca aagagaaaag ggcggcctcg gacagcgagg ctgccctttg tgctccccgg
    10261 ggacagcgct cagcgcgccc ggcgcgccgt ccgcatcgcc gccgccaggt ccaggtcggc
    10321 gatgaccaca tgcccacctc gctccaccaa cagttgagcc agggcccggc cgatgccatt
    10381 tgccccgccc gtcacgatgc tgacctgggg t
//
LOCUS       DAOPJG010000119        10393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6733_length_10393_cov_7.490714, whole genome shotgun sequence.
ACCESSION   DAOPJG010000119 DAOPJG010000000
VERSION     DAOPJG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6733_length_10393_cov_7.490714"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /gene="pgk"
                     /locus_tag="PKU75_11105"
     CDS             <1..361
                     /gene="pgk"
                     /locus_tag="PKU75_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPF81472.1"
                     /translation="VVDADKIPADMMGLDIGPKSAELYASKLADAKTVFWNGPMGAFE
                     MAPFAAGTKTVAQALVDATAAGALTVVGGGDSAAAVRQMGFADSDFGHISTGGGASLE
                     YLEGKALPGITILEGIK"
     gene            358..1152
                     /gene="tpiA"
                     /locus_tag="PKU75_11110"
     CDS             358..1152
                     /gene="tpiA"
                     /locus_tag="PKU75_11110"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPF81473.1"
                     /translation="MSRTPLMAGNWKMNLDHQAATLLVQKLDWTLKDANHDFGAVEVA
                     VCPPFTDLRTVQTLVDGDRLEIKYGGQDLSVHDSGAYTGEISGAFLAKLGCTYVIVGH
                     SERREYHNETDEVVADKLAAAYRYGLTPIFCCGEGLEIRKAGTHVAHVLDQIRAGLAQ
                     LTPEQVRSIVIAYEPIWAIGTGEVATPDDAQEVCGAIRTQLAEMYTGDTADGIRILYG
                     GSVKSSNIAQIMGGRDVDGALVGGAALDAAEFAAICRYQQHVVPDA"
     gene            1366..1611
                     /gene="secG"
                     /locus_tag="PKU75_11115"
     CDS             1366..1611
                     /gene="secG"
                     /locus_tag="PKU75_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPF81474.1"
                     /translation="MDAVRIGLQVLLVISGLFLTLLILLHKGKGGGVSDMFGGGLSTS
                     MSSSSVAEKNLTRITIMMALVWVTAVVGIGLIDRFNA"
     assembly_gap    1819..1918
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1957..2307
                     /locus_tag="PKU75_11120"
     CDS             1957..2307
                     /locus_tag="PKU75_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HPF81475.1"
                     /translation="MAGGNAIRGSRVGAGPMGEAERGEVAARVFVSYYCANNHETRPS
                     FAEEPGLVIPEFWDCPRCGFPAGQDKDNPPQPPKVEPYKTHLAYVKERRSDTDGAAIL
                     DEALGSLRDRGLIK"
     gene            complement(2324..3505)
                     /locus_tag="PKU75_11125"
     CDS             complement(2324..3505)
                     /locus_tag="PKU75_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HPF81476.1"
                     /translation="MIVDLPSTTTADISKKLIALRQDVGAMALSRVLTLVVVTDEQGA
                     DAAIETANTASRQHPSRIIVVITANKRGAARLDAQIRVGGDAGASEVVVIRLYGPLAA
                     HGRSVVTPLLLADSPIVVWWPGEPPKDPGADPIGAMAQRRVTDSAKSAAKPKAVLGRL
                     AAAYTDGDTDLAWSRITLWRGLLAAALDQAPYEPVTEALVVAAPDSPSGDLLAGWLAA
                     YLKCPVALVRGAHRSGIISVRLTRKSSTIDLVRPQEGNVATAIQTGQPERTIALAHRK
                     DAECLADELRRLDSDEVYEEALRRGIPKITAKTMTAGAAVRSGLAVSEKEAMEIANRV
                     HRKNRLGKAASMVTELPDASDDEQRVKVAAAEKLAASTKTDLEPPSEPTSRAGRAATK
                     R"
     gene            complement(3564..5108)
                     /gene="zwf"
                     /locus_tag="PKU75_11130"
     CDS             complement(3564..5108)
                     /gene="zwf"
                     /locus_tag="PKU75_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPF81477.1"
                     /translation="MSPARVAPGHNPLRDPRDKRLPRIAGPCALVLFGVTGDLATKKL
                     MPAIYDLANRGLLPPGFSLVGFARRDWADQDFGKIVYESIKARSRTPFREEVWRSLAE
                     GIRFVPGTFDDDNAFDLLAATLAELDEMRGTGGNTAFYLSIPPGFFATVCEQLERAGL
                     STPKEGSWRRVVIEKPFGHDLKSARELNAIVEGVFPADAVFRIDHYLGKETVQNLLAL
                     RFANQMFEPVWNSNYVDHVQITMAEDIGIGARAGYYDGIGAARDVIQNHLLQLMALTA
                     MEEPVSFDADGLRLEKEKVLGAVRIPKDLATGTARGQYAEGWQGAEHVVGFLQEKGVP
                     NTSTTETYAAVRLDVETRRWAGVPFYLRTGKRLGKRVTEIAVVFKRAPHLPFTDTATE
                     ELGQNAVVIRVQPDEGVTLRFGAKVPGAQMEVRDVTMDFGYGRAFTESSPEAYERLIL
                     DVLLGEPPLFPRHEEVELSWQILDPIEAFWARQGTKPDQYPSGGWGPKSADELMRRDG
                     REWRLP"
     gene            complement(5207..6319)
                     /gene="tal"
                     /locus_tag="PKU75_11135"
     CDS             complement(5207..6319)
                     /gene="tal"
                     /locus_tag="PKU75_11135"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPF81478.1"
                     /translation="MTDNANLTALADAGVSVWLDDLSRERLTSGNLKDVMEHSGVVGV
                     TTNPSIFQAALDHGNAYDAQVAELAEAGKSVEEAVFEITTQDVRDACDVLRPIYDKTG
                     GKDGRVSIEVDPRLAHDAGPTVEQAKALYAKVDRPNVMIKIPATLAGLPAISEVIADG
                     ISVNVTLIFSLERYRAVMNAYLEGLERALAAGKDLSTIWSVASFFVSRVDTEIDKRLE
                     AIGSPEAMALRGTAAVANAQLAYQAYEEVFSTSRWETLAAEGANKQRPLWASTGVKNP
                     DYSDTLYVTNLVADNTVNTMPEKTLDAVIDHGNIAGDAVTGHYADAAKVLDDIAAQNI
                     SYTEVTELLEKEGVEKFEASWNSLLTDLQAALDAAK"
     gene            complement(6349..8574)
                     /gene="tkt"
                     /locus_tag="PKU75_11140"
     CDS             complement(6349..8574)
                     /gene="tkt"
                     /locus_tag="PKU75_11140"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPF81479.1"
                     /translation="MTAASTAPARKRAAKKAPTRSVPAPKATKAGWSDLDVRAVDTVR
                     VLAADAVQKVGNGHPGTAMSLAPVAYLLFQNVMRIDPNDEHWLGRDRFVLSCGHSSLT
                     QYIQLYLSGYGLELDDLKALRTWGSKTPGHPEVHHTTGVEITTGPLGSGLASAVGMAF
                     AQRRQRGLFDPDAKKGTSPFDHHIWVIASDGDIMEGVTHEASALAGHQELGNLTVIYD
                     QNTISIEDKTEISFSEDVGARYAAYGWNVVEIDWRKDGDGATYVEDVDALLAACEKSR
                     DATKPTIIVLRTIIAWPSPTKQDTGKSHGSALGGDEIAGLKEVLGFDPKKTFEVSREV
                     ITHTRKVKARGKALHKEWDKRYAAWRKGNAEGAALLDRLVSQELPAGLDKAVPSFPAD
                     PKGIATRAASGKVLGALAEVVPELWGGSADLAESNNTTMEGQPSFIPASKQTREWSGG
                     PYGRTLHFGIREFGMGLILNGIALEGLMRPYGGTFLVFSDYMRAAVRLAAIQELPVTF
                     VWTHDSIGLGEDGPTHQPIEHLASLRAIPGFEVVRPADANETAAAWVQILKNAKPVGL
                     CLSRQALPTQDPKVAGKISNVGKGAYILREASSGTPDVILIATGSEVGLAMAAADTLE
                     KGKIATRVVSMPCREWFETQTKGYQEKVLPAAVKARVSVEAGVPMGWRDLVGDAGEIV
                     GIDHFGASADAATLFREFGFTPEKVVAAAKRSIKAAAAAAGPDAPQPSSADTDVDAGK
                     S"
     gene            complement(8699..9130)
                     /locus_tag="PKU75_11145"
     CDS             complement(8699..9130)
                     /locus_tag="PKU75_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81480.1"
                     /translation="MLSVLAAVSYGAVSAFVPVVNAEVYVVAAARASVGTALAVALAL
                     AVGQSAGKVVIFETARRGAGCFAGKRATPRWAALVRNGFRRRRTAIPLVLAAATVGVP
                     PLAAVSLAAGAAGQRRWEFTVLCLTGRAARFVALAVPLALA"
     gene            complement(9124..9729)
                     /locus_tag="PKU75_11150"
     CDS             complement(9124..9729)
                     /locus_tag="PKU75_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPF81481.1"
                     /translation="MTRLLASPRTPPATGLPVGPLRTVPNYVTLVRSALAVALGTVAL
                     STGDLALLAVAYAVYWAGDILDGWSARRLGQETRLGAVLDIVGDRACTAILCGGLVTH
                     VSGVVPVAVVFLLTFLVLDAMLSLAFLCWPLLSPNYFYRVDAGVWRLNWSTPAKAANT
                     AGVVGALLLGLSWAALAIAVGVFVVKLWSAARVLRLLDARC"
     gene            9854..>10393
                     /locus_tag="PKU75_11155"
     CDS             9854..>10393
                     /locus_tag="PKU75_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81482.1"
                     /translation="MRLDVSTVRAAAYAVAVAAACVSSAAVSRGGAGADFVQFLAPLS
                     LLLAWWLPERRPLILAGAIAIIGAGVADDGAVGVSVAGLAFLGVAEDRRARPWRVALG
                     GGLAGGILAAGLRGDPAGLFGVLGSVAVGGGAALLLRYWERTSELTSETRELRSKAAW
                     LEQRTALARELHDVVGHQVT"
BASE COUNT         1541 a         3478 c         3622 g         1652 t
ORIGIN      
        1 cgtcgtcgac gccgacaaga tcccggccga catgatgggc ctggacatcg gcccgaagtc
       61 cgccgagctc tacgcgtcca agctggccga cgccaagacg gtcttctgga acggccccat
      121 gggtgccttc gagatggcgc ccttcgctgc cggcaccaag accgttgcgc aggccctcgt
      181 cgacgcgacg gccgccggtg cgctcaccgt cgtcggcggt ggcgactcgg ccgctgccgt
      241 gcggcagatg ggcttcgccg acagcgactt cggtcatatc tccaccggtg gtggggcctc
      301 cctcgaatac ctcgagggca aggcgctgcc gggcatcacg atcctggaag gcatcaaatg
      361 agccgcaccc cgctgatggc gggcaactgg aagatgaatc tggaccacca ggcggcgacg
      421 ctgttggtgc agaaactcga ctggaccctc aaggacgcca accacgactt cggcgccgtc
      481 gaggtcgcgg tctgcccgcc gttcaccgac ctgcgcaccg tgcagaccct ggtcgacggc
      541 gacaggctgg agatcaagta cggcggtcag gacctctcgg ttcacgactc cggtgcttac
      601 accggcgaga tctccggcgc cttcctcgcc aagctcggct gcacctatgt catcgtcggg
      661 cacagcgagc ggcgcgagta ccacaacgag accgacgagg tcgtcgcgga caagctcgcc
      721 gcggcatacc gctatggctt gacgccgatc ttctgctgcg gcgagggcct ggagatccgc
      781 aaggccggca cccatgtcgc gcacgtcctg gatcagatcc gggccggcct ggcgcagctg
      841 acgccggagc aggtgcgctc catcgtcatc gcctatgagc ccatctgggc catcggcacc
      901 ggcgaggtcg ccacccccga tgacgcccag gaggtctgcg gcgccatccg cacccagctc
      961 gccgagatgt acaccggcga caccgcggac ggcatccgca tcctctatgg cggctcggtc
     1021 aagtcgagca atatcgccca gatcatgggc ggcagggacg tcgacggcgc cctcgtcggc
     1081 ggcgccgccc tcgacgccgc cgagttcgcc gcgatctgcc gctaccagca gcacgtcgtc
     1141 cccgacgcgt agatcgccgg agtcacaact tttccgccca gggcggaaaa ctagcggtgc
     1201 gcaacggaac atttccgttg cgcaccgcta tgttcccgcc caaaaccccg ccgtatgccg
     1261 ggggaaccgg tccgcgcagc cggcgcgttg ggacgggtag gccgcggcag gtatcctttt
     1321 tcggttggcc gcggccggcc acactcgatc gaaaggttgg aaccgatgga tgcggtgcgc
     1381 atcggactgc aggtgctgct tgtcatctcc gggctcttcc tgaccctgct catcctgctg
     1441 cacaagggca agggcggtgg cgtgtccgac atgttcggtg gcggcctgtc gacctcgatg
     1501 tcctcttcct ccgtggccga gaagaacctg acccgcatca cgatcatgat ggccctcgtg
     1561 tgggtcaccg ccgtggtcgg catcggcctg atcgaccgct tcaacgcctg agggccggac
     1621 cgggcgccac accgaccggg ctcaagaggt ggggcgatca ccccacgtcc ggatgggttg
     1681 cccgtcgaat gaacccgcag ccgcgggtcc gccggactcg tgactgatac ggatcgcacc
     1741 tggaaccggg tcgccccaca cacatctcac ccgtctcgac gtacactcca ctcgacactg
     1801 cacaggtcat gacgtcagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     1921 cacaggtcat gacgtcagag aggacgaacg ggaactatgg ctggtggtaa tgccattcgc
     1981 gggagccggg tgggtgccgg accgatgggt gaggccgagc gcggcgaagt cgccgcccgc
     2041 gtcttcgtct cctactactg cgccaacaac cacgagaccc ggcccagctt cgcggaggag
     2101 cccggcctcg tcataccgga attctgggac tgccctcgct gtggcttccc cgccggtcag
     2161 gacaaggaca acccgcccca gccgcccaag gtcgagccct acaagactca cctggcctat
     2221 gtgaaggagc gccgctccga caccgacggc gccgccatcc tcgacgaggc gctcggcagc
     2281 ctccgcgacc gtggcctgat caagtaacgc gcgtctgact gcgtcagcgc ttggtggccg
     2341 cccggcccgc tcggctggtg ggctccgaag gtggctccag gtcggtcttg gtcgacgcgg
     2401 ccaacttctc ggcggcggcc accttcacgc gctgctcgtc atcgctcgcg tcgggaagtt
     2461 cggtgaccat cgacgccgcc ttgccgagac ggttcttgcg gtgcacgcgg ttggcgatct
     2521 ccatcgcctc cttctccgag accgcgagtc ccgaacgaac ggccgcgcct gcggtcatcg
     2581 tcttggcggt gattttcggt atgccgcgcc gcagcgcctc ctcgtagacc tcgtcggagt
     2641 ccagccggcg cagttcgtcg gcgaggcact cggcgtcctt gcggtgcgcc agggcgatgg
     2701 tgcgctcggg ctggccagtc tggatggccg tggcgacatt gccctcctgc gggcgcacca
     2761 ggtcgatcgt gctggacttg cgggtcaggc gcacggagat gatgccgctg cggtgggcgc
     2821 cgcggaccag cgcgacgggg cacttcaggt aggcggcgag ccagcccgcc aggaggtcgc
     2881 cggacggtga atccggggcg gcgacgacga gggcttcggt caccggctcg tagggcgcct
     2941 ggtccagggc agcggcgagc aggccgcgcc acagggtgat ccgcgaccag gcaaggtcgg
     3001 tgtcgccgtc ggtgtatgcc gcggcgagcc ggccgaggac cgccttgggt ttcgcggccg
     3061 acttcgccga gtcggtgacc cgccgctgcg ccatcgcgcc gatgggatcc gcgccggggt
     3121 ccttcggcgg ctcacccggc caccagacga cgatggggga gtcagccagc aggaggggcg
     3181 tgacgaccga gcggccgtgt gcggccaggg gcccgtagag gcggatgacg acgacctcgc
     3241 tggcgccggc atccccgccc acccggatct gggcatccag ccgtgcggcc cctcgcttgt
     3301 tggcggtgat gacgacgatg atccgggagg ggtgctgccg gctcgcggtg ttcgccgtct
     3361 cgatcgccgc gtcggcgccc tgttcgtcgg tcacgacgac gagggtgagc acgcgcgaga
     3421 gcgccatggc gccgacgtcc tggcgcagcg cgatgagctt cttggagatg tcggcggtcg
     3481 tggtgctggg caggtcgacg atcacttccg gggtccccga tcagtatcga agcgacggag
     3541 gagcgcagaa cttattgggg tggtcatgga agcctccatt cgcggccgtc gcggcgcatc
     3601 aactcatcgg cggacttggg cccccagccg ccggagggat attggtcggg tttggtgccc
     3661 tggcgcgccc agaaggcctc gatcgggtcg aggatctgcc aggacagctc gacctcctcg
     3721 tggcgcggga acagcggcgg ctcgcccaag aggacatcga gaatgaggcg ctcgtaggcc
     3781 tcgggagagg actcggtgaa ggcacggcca tacccgaaat ccatcgtcac atcgcgcact
     3841 tccatttgcg ccccaggcac tttcgcgccg aagcgcaagg tgacgccttc gtcgggctgg
     3901 acccggatga cgacggcgtt ctggccgagc tcttcggtcg cggtgtcggt gaacggcagg
     3961 tgcggtgccc tcttgaagac gacggcgatc tcggtcacgc gtttgccgag ccgcttgccg
     4021 gtgcgcagat agaagggcac ccccgcccac cggcgggtct ccacgtcgag gcggaccgcg
     4081 gcatacgtct cggttgtcga ggtgttgggg acgcccttct cctgcaggaa tcccacgacg
     4141 tgttcggcgc cctgccaccc ctcggcatac tgcccgcggg cggtgccggt ggccaggtcc
     4201 ttcggaatgc gaaccgcccc aaggactttt tccttctcca agcgcagccc gtcggcgtcg
     4261 aacgagaccg gttcctccat cgcggtgagc gccatcagct gcagcaggtg gttctggatg
     4321 acgtcgcggg cggcgccgat gccgtcgtag tagccggccc gggcgccgat gccgatgtcc
     4381 tcggccatcg tgatctgcac gtggtcgacg tagttgctgt tccacaccgg ctcgaacatc
     4441 tggttggcga agcgcagcgc cagcaggttc tggaccgtct ccttgccgag atagtggtcg
     4501 atcctgaaga ccgcgtcggc cgggaagaca ccctcgacga tggcgttgag ttcgcgggcg
     4561 ctcttgaggt catgcccgaa cggtttctcg atcacgaccc ggcgccacga gccctccttc
     4621 ggcgtcgaca acccggcacg ctcgagttgc tcgcagacgg tggcgaagaa gccgggcggg
     4681 atcgagagat agaaggccgt attgccgccg gtgccgcgca tctcgtccag ttcggcgagc
     4741 gtggcggcaa gcaggtcgaa agcgttgtcg tcgtcgaaag tccctgggac gaaacggatt
     4801 ccctcggcca gggagcgcca cacctcctcc cggaagggcg tgcgggaacg cgccttgatc
     4861 gactcgtaga cgatcttgcc gaagtcctga tcggcccagt cgcgccgggc gaacccgacg
     4921 aggctgaagc ccggcggcag cagaccccgg ttggccaggt catagatcgc cggcatcagc
     4981 ttcttggtcg ccaggtcgcc cgtgacgccg aaaagcacca gggcgcaggg tccggcgatc
     5041 cgcggcagcc gtttgtcgcg aggatcgcgc aggggattgt gaccaggagc gacacgggcg
     5101 ggactcatcg cgtcctttca ggtgggagcg gaggacttct ccggtgtctt ctcggggagt
     5161 cttggcggcg ggcgccggct tgcgccgacg cccgccacga tgggggttat ttggccgcgt
     5221 ccagtgcggc ctggaggtcg gtgaggaggg agttccagga cgcctcgaac ttctcgacgc
     5281 cctccttctc cagcagctcg gtcacctcgg tgtacgaaat gttctgtgcc gcaatgtcat
     5341 cgagcacctt ggccgcatcg gcgtaatgcc ccgtcacggc gtctccggcg atattgccgt
     5401 ggtcgatgac tgcgtcgagg gtcttctcgg gcatggtgtt gacggtgttg tccgcgacca
     5461 ggttggtgac atagagggtg tcggagtagt ccgggttctt cacgcccgtc gatgcccaca
     5521 acggccgctg cttgttggcg ccctcggccg cgagcgtctc ccagcgcgac gtcgagaaga
     5581 cctcctcgta ggcctggtag gccagctggg cgttggcgac agcggccgtg ccgcgcaatg
     5641 ccatcgcctc cggagaaccg atcgcctcca gccgcttgtc gatctcggtg tcgacccggc
     5701 tgacgaagaa ggaggcgacc gaccagatcg tcgagaggtc cttgccggcg gcgagcgcgc
     5761 gctccagccc ctcgagatag gcgttcatga cagcgcgata gcgctcgagg ctgaagatca
     5821 gggtcacgtt gacgctgatg ccatcggcga tgacctcgga gatcgccggc agccctgcga
     5881 gagtggccgg gatcttgatc atcacattgg gccggtcgac cttggcgtaa agcgccttgg
     5941 cctgctcaac cgtcggcccc gcgtcatggg ccagtcgcgg gtcgacctcg atggagacgc
     6001 gcccgtcctt gccgccggtc ttgtcgtaga tcggcctgag gacatcacac gcgtcgcgca
     6061 cgtcctgggt cgtgatctcg aacaccgcct cctcgacgga cttgcccgct tcggcgagtt
     6121 cggccacctg cgcgtcatac gcattgccgt gatccaaggc ggcctggaag attgacgggt
     6181 tcgtcgtgac gccgacgacc ccgctgtgtt ccatcacgtc cttgaggttg ccggaggtga
     6241 ggcgttcgcg ggacaggtcg tccagccaca ccgagacgcc ggcgtcggcc agggcggtca
     6301 gattggcatt gtcagtcatc tgttctgctc cttgtgggta tgccgtgctc aggacttgcc
     6361 cgcgtccacg tcggtgtcgg cggagctggg ttgcggtgcg tcgggtcccg cggccgcggc
     6421 ggccgccttg atgctgcgct tcgcggcggc gacgaccttc tcgggcgtga aaccgaactc
     6481 gcggaacagg gttgcggcat ccgcggaagc gccgaagtgg tcgatgccga cgatctcgcc
     6541 cgcgtcgccg acgaggtcgc gccagcccat cggcaccccg gcctcgaccg agacgcgcgc
     6601 cttgacggcg gcggggagca ccttctcctg gtagcccttg gtctgggtct cgaaccactc
     6661 gcggcacggc atggagacga cacgggtggc gatcttgccc ttctcgagcg tgtcggctgc
     6721 tgccatcgcc aggccgacct cggagccggt ggcgatgaga atgacgtccg gtgtgccgga
     6781 ggaggcttca cgcaggatgt aggcgccctt gccgacattc gagatcttgc cggcgacctt
     6841 ggggtcctgc gtcggcaacg cctgccgcga caggcacagg ccgaccggct tggcgttctt
     6901 gaggatctgc acccaggccg ccgccgtctc gttggcgtcg gccggccgca ccacctcgaa
     6961 acccgggatc gcgcgcagcg aggccaggtg ctcgatcggc tggtgtgtcg ggccgtcctc
     7021 gccgaggccg atggagtcgt gcgtccacac gaaggtgacg ggcagttcct ggatggccgc
     7081 cagccgcacg gcggcgcgca tgtaatcgga gaagacgagg aaggtgccgc cgtaggggcg
     7141 catcaggccc tcgagggcaa ttccgttgag gatcaacccc atcccgaact cgcggatgcc
     7201 gaagtgcagg gtgcggccgt agggcccgcc cgaccactcc cgcgtctgct tggaggccgg
     7261 gatgaaggac ggctgcccct ccatcgtcgt gttgttggat tccgccaggt cggccgatcc
     7321 gccccacagc tcgggcacca cctcggcgag cgcgccgagc accttgcccg aggcagcgcg
     7381 ggtggcgatg cccttggggt cggcggggaa ggacgggacg gccttatcga gaccggccgg
     7441 aagctcttgg gagacaaggc gatccaggag cgcggcgccc tcggcgttgc ccttgcgcca
     7501 cgcggcatac cgcttgtccc actccttgtg cagcgccttg ccgcgggcct tgaccttgcg
     7561 ggtgtgggtg atgacctcgc gggagacctc gaaggtcttc ttcgggtcga accccaggac
     7621 ctccttcagg ccggcgatct cgtcgccgcc gagcgcggac ccgtgcgatt tgccggtgtc
     7681 ctgcttggtc ggggaaggcc acgcgatgat cgtgcgcaac acgatgattg tcggtttcgt
     7741 ggcgtcccgg gacttctcgc aagccgccag cagggcgtcg acgtcctcga cgtaggtcgc
     7801 gccgtcgccg tccttccgcc agtcgatctc gacgacattc cagccgtatg ccgcgtagcg
     7861 ggcgccgacg tcctcggaga aggagatctc ggtcttgtcc tcgatcgaga tcgtgttctg
     7921 gtcgtagatg acggtcaggt tgccgagttc ctggtggccg gcgagcgcgg aggcctcgtg
     7981 agtgacgcct tccatgatgt cgccgtcgga cgcgatgacc cagatgtggt ggtcgaaggg
     8041 gctggtgccc ttcttggcgt ccggatcgaa caggccgcgc tggcggcgct gggcgaaggc
     8101 catcccgacg gccgaggcaa ggccggaccc gagcgggccg gtggtgatct cgacgcccgt
     8161 ggtgtggtgc acttccggat gcccgggggt cttcgagccc caggtgcgca gcgccttgag
     8221 gtcgtcgagt tcgaggccgt agccggagag gtagagctgg atgtactgcg tcagcgagga
     8281 gtgcccgcac gacaggacga agcggtcgcg gccgagccag tgctcgtcgt tggggtcgat
     8341 gcgcatgacg ttctggaaga ggagataggc gacgggcgcc aggctcatcg ccgtcccggg
     8401 gtggccgttg ccgaccttct gcaccgcgtc ggcggccagc acccggacgg tgtcgacggc
     8461 acgcacatcc agatcgctcc accccgcctt cgtcgccttg ggcgccggca ccgaacgggt
     8521 cggggccttc ttggcggcgc gtttgcgggc gggcgcggtg gacgctgcag tcacggttgt
     8581 ccttcctgga cgggcgtgcg ggatagatct gctcggttca gcctaatgcg tgtgacgcgc
     8641 gccgcagcgg cggtgatcgg ccgacttgcg atgggccggt ccgcatatcc ggcgcgactc
     8701 acgccagcgc gagcggcacc gcgagcgcga cgaagcgcgc ggcccggccg gtcagacaca
     8761 acacggtgaa ctcccaccgc cgctggcccg ccgcccccgc ggccaggctc accgccgcca
     8821 gcggcggcac accgacggtc gcggcggcca atacgagagg tatggccgtg cgccggcgcc
     8881 ggaatccgtt gcgcacaagg gcagcccagc gcggggtcgc ccgcttccca gcgaagcatc
     8941 cggccccgcg ccgcgccgtc tcgaagatga cgaccttgcc ggcgctctgg cccaccgcca
     9001 gtgcgagcgc caccgcgagc gccgtgccca cactcgctcg ggcggcggcg acgacataca
     9061 cctcggcatt gacgacaggc acgaacgccg agaccgcgcc atagctcaca gcggccagga
     9121 cgctcagcat cgcgcgtcca gcaggcgcag cacgcgggcc gcggaccaca gcttgacgac
     9181 gaacacgccg accgcgatcg ccaaggccgc ccacgacagg ccgagcagga gggcgccgac
     9241 gacgccggca gtgttcgcgg ccttggccgg cgtcgaccag ttcaaccgcc acacaccggc
     9301 gtcgacgcgg tagaagtagt tcgggctgag cagcggccag cagaggaacg ccagggacag
     9361 catcgcgtcg agcacgagga aggtcagcag gaagacgacg gcgaccggga cgacgccgct
     9421 gacgtgcgtg accagcccac cgcacaggat ggccgtgcac gcgcggtcgc cgacgatgtc
     9481 gaggaccgcg cccaaccggg tctcctggcc cagccggcgc gccgaccagc cgtcgaggat
     9541 gtcgccggcc cagtagacgg catacgccac cgcgagcagc gcgagatctc cggtgctgag
     9601 cgcgacggtc cccagcgcga cggcgagtgc gctgcgcacc agcgtcacat agttcggaac
     9661 cgtgcgcagc ggaccgactg gcaggccggt tgccggcggc gtgcggggcg aagcgagtaa
     9721 gcgggtcatg cgccgacgct ctcgcgcccg accgggtatg cggatcggcc gccggtgccg
     9781 agccggccgg tacccgggta ccgagtccgc tcgggcggct gacagccggg gttccgaggc
     9841 ccagactgtc ggtgtgcgcc tcgacgtctc gaccgtgcgc gccgctgcgt atgcggtggc
     9901 cgtcgccgcc gcctgcgtca gttccgcggc cgtgagccgg ggcggcgccg gcgccgactt
     9961 cgttcagttc ctcgctccgt tgagcctgct cctggcctgg tggctccccg agcgccgccc
    10021 gctcatcctg gccggcgcca tcgcgatcat cggggccggg gtcgccgatg acggcgccgt
    10081 cggggtcagc gtcgcggggc tggccttcct gggagtcgcc gaggaccgcc gggcccggcc
    10141 gtggcgggtg gcgctcggtg gtggcctggc cggcgggatc ctcgccgcgg ggctgagagg
    10201 ggacccggcc ggcctgttcg gggtgctggg cagtgtcgcc gtcggcggcg gcgcggcgct
    10261 gctgctgcgc tattgggagc gcacgagcga gctgacgagc gagacccgcg aactgcgcag
    10321 caaggcggct tggctcgagc agcgcaccgc cctcgcccgt gagctgcacg acgtcgtcgg
    10381 tcaccaagtg acg
//
LOCUS       DAOPJG010000120        10308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6811_length_10308_cov_7.635619, whole genome shotgun sequence.
ACCESSION   DAOPJG010000120 DAOPJG010000000
VERSION     DAOPJG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6811_length_10308_cov_7.635619"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKU75_11160"
     CDS             <1..213
                     /locus_tag="PKU75_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81483.1"
                     /translation="SPSWCIGPTSERPGGVTDALWDKVRPEAQAQVKACAAAGPKMDL
                     KRCPRSMALTFALSRIGVRLLAEPTH"
     gene            301..861
                     /gene="efp"
                     /locus_tag="PKU75_11165"
     CDS             301..861
                     /gene="efp"
                     /locus_tag="PKU75_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPF81484.1"
                     /translation="MATTNDLKNGLVLNIDGQLWTVIEFQHVKPGKGPAFVRTKLKNV
                     LSGKVVDKTFNAGVKVETANVDKRTMQYLYNDGSDFVFMDPDTYDQIHVGPDVVGDAA
                     KYMLENQDAIVARHDGSVLYIELPASVVLEISYTEPGLQGDRSTGGTKPATLETGAEI
                     QVPLFLEAGTKVKVDTRDGSYLGRVN"
     gene            884..1297
                     /gene="nusB"
                     /locus_tag="PKU75_11170"
     CDS             884..1297
                     /gene="nusB"
                     /locus_tag="PKU75_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPF81485.1"
                     /translation="MAARTKARKRALDLLFEAEARGVNVGDLLAERLDAPVTEAPLNP
                     YTATLVEGVVAHWRDINELLNTYSQGWPVERMPAVDRAILRIGAYEVLYSDEVPDKVA
                     IAEAVALAKDLSTDESPKFVNGLLARLLQVKPTLA"
     gene            1381..3213
                     /locus_tag="PKU75_11175"
     CDS             1381..3213
                     /locus_tag="PKU75_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPF81486.1"
                     /translation="MRIRSPAVDGVTVFAVVGVNTVSFGLRVSAGARKGLLGFAVQRR
                     SAGGRWRYVEGFKVFRSLTPDPEPGATHSTRRHPIQSLVWDDFTVRENGSYDYRFIPF
                     RGTPAEPRYGTPVEITVRSEPLWGERHTIVFNRGVASSQAYQSRFGGLAPDEQPTAAA
                     RQLALDWLSRDLDETFLAVIRGAQPGDALRGAFYEFTYAPVLSEFAAAIERGVDVRLV
                     LDLKVNEHWENVRTPDGTTTRTWVESAPREASLRALSDAGIPASAVVARQARANDLQH
                     NKFLVHIRKGEPAAVWTGSTNVTLGGIHGQSNVGHHVRDPELAAGFLAYWEILAADPG
                     ARAGMTRGEVIRANREFEAAVDALSAVPGSRSSIPTGVSAIFSPRSTPAALELYADLL
                     AHPDRLACATFAFGIAKELRAVLEQGDEDSPLRFFLLEKRTLSTQSTSSAATVELDWR
                     RNIYLAWGSELGTPLGQWVAETTTRELGLNRHVAFIHDKFLLGDPLGADPVVVTGSAN
                     FSLASSVDNDENMLLIRGDTRVADIYFTEFNRLFFHYYFRSVVDSLSGRDRAGARRSA
                     PSALDLVEDDSWLAKYAPGTLRTKRAEQFVRMAGAQLSAATSET"
     gene            complement(3400..3633)
                     /locus_tag="PKU75_11180"
     CDS             complement(3400..3633)
                     /locus_tag="PKU75_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81487.1"
                     /translation="MRTTLELDDVVLSAARATARAEGISLGRAVSDIALAGLALPTAT
                     DGPASAGDGIPVLRGVPGHVVTDDLVAAFRDAD"
     gene            3714..4877
                     /locus_tag="PKU75_11185"
     CDS             3714..4877
                     /locus_tag="PKU75_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81488.1"
                     /translation="MPARLSFTAIRYADAADIPLIEGIERAGDTRFAEVSRARGIDPG
                     LFPGVGMPGPERAASPGLVLVAGQPAVGFAHIVPVAGCLVLEQLSVSPDAGRRGVGGS
                     LLHAAMGAALDMGHDTLYLRTFAEVPWNAPFYARSGFLEIPDPEWIAPLVAAEDGAGL
                     RRWGRRITMACPLADEPVPRPAVSVIPLKDGPRGLEAFVQYRVSTMDFAAGAVVFPGG
                     RVDPVDHDAAITLPQTTLDEQADRWAATDFRKLGARPGDAARTILATGVREVREEAAA
                     VIDPATLIPWDNWVTPTGISRRFDVYFLIAPVNEGAAGEWRHTTSEAHDSHWTPVEAL
                     AERAERGELLLLPPTRAIVDELLALGDLSSVLALTPAIRAVRHDIDIRRPRGG"
     gene            4912..6759
                     /locus_tag="PKU75_11190"
     CDS             4912..6759
                     /locus_tag="PKU75_11190"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HPF81489.1"
                     /translation="MGANHTAYPRRAQRATAALAALALTGLVVSVSAPGAASADSGEP
                     TMMMPTGGGYEPVALQAFGKAAISKADDATVDIVVVPSAYGDDLPSRPENIALARERT
                     AEIAAACNEVVTGTGKRCVGRLAILLNRADALNAANSAALRNPSLDGIYILGGDQGIA
                     MKVLANSPAERAMAAAVTGGRVVLAGTSAGAAVESRSMINGYVGDYGPAQGLRRGSTL
                     MWWGDDSDAERGLAFGSRKAIYDQHFYQRGRFGRSMSTIATADERFGGTSPVGVGADY
                     ATGVVNYGDKLLTQVFGDSGVGTIDFESLGATHRWVGTERWLSARKVKVNLMTEGTDF
                     DLDSRRLTRNGALVGQVSGRAWSAPAAPGRGVLYLGGGILQTTKVMGSVVKDAASASP
                     APADARLLILSADSSSTAADYGALAKRAGWMGHIDTAVYGSASWAGVDIGRYAAVLVV
                     AVAPPKSVAAFADARFRGLVTTAVATAPVVFADGPAAAYLGSRWSAQARPNGDDYEDK
                     AVAAFKTTEAKWRAGTGSVAATVIPAINTDYQWGRAFNAVAVAKGQLALGVSAGAAIR
                     LQGGAARVVDGSVVVVDGRDATTWASSNGTLGAAGLVLDTFGPGEALRR"
     gene            complement(6883..8847)
                     /locus_tag="PKU75_11195"
     CDS             complement(6883..8847)
                     /locus_tag="PKU75_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81490.1"
                     /translation="MKRAGMLVCAGAGLIALVVPGAASAGNAGSPDRSEGLSAAESAR
                     LDAGVQLAPLRSGATGPSPFTAYLPDLHKTNFAAWVAKVAKISAAAATTPERQAAKAR
                     WSGASDRAVLSYREKEAATVIGANDTTWKGEKVSAFGLGAGLHNRMVVSGTLADKRPA
                     LRSLPMAKEDNGSIPKAAATGIDGNGRVKITGRIGDGPYGTSTGDFDWYKLTAKPGET
                     VGAAIPSSSLDVFGLLVDGHGTVLDVAWHTPDGSLPDKTASTTSTAGGTYYLLLLDPD
                     TWQMDFSDSASGTGAANSGNYTAEFGAYRTDRDAVLVKLRRGDVLGTSVTGAAGRVSV
                     QKWDSPYVTASSQSDTTAYYPPNSPLPGGGNAATAYVAEADGWYGVITETGIGAYRNA
                     VEVYRPGGESTSAPQTIYLDFDGASINTSIFGGPDMQAKLSPLSSFLKGWGLTQADLP
                     ALTDAITKTVTENLRDDMRRKNIPNLNVRIVNGRTSPDTFGRPNVSRVIVGGTIDESQ
                     IPTVGIAEHIDPGNYGREDTALVLQDLMSAPVKGNPSAVVSLNYYLRPGVGNKVQLIG
                     RAVGNVVAHEVGHYIGNWHTDNSNSVKSIMDSGGRGFWRMFNAGPDWVLGTADDGDTD
                     FTTDRYSLNEPFFGREDTANVTAWAFSGKR"
     gene            9061..9651
                     /gene="pyrR"
                     /locus_tag="PKU75_11200"
     CDS             9061..9651
                     /gene="pyrR"
                     /locus_tag="PKU75_11200"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPF81491.1"
                     /translation="MTRPDSSGPPAGSPVDPHGRTVLSSNEIARGLRRIAHEILEHNK
                     GAADLVLLGIPSRGVELAERLALILAEVEGCDPLPVGSLDITMHRDDLRHQPTRSPMR
                     TVIPDCGVDDKIVVLVDDVLYSGRTIRAALDALADLGRPRAVRLAVLVDRGHRELPIR
                     ADHVGKNLPTSHSEKVQVRLRGHDGVEDVVRISGGN"
     gene            9654..>10308
                     /locus_tag="PKU75_11205"
     CDS             9654..>10308
                     /locus_tag="PKU75_11205"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPF81492.1"
                     /translation="MPKHLISSADLDRGEVEAILETASSMHDVQRREVKKLPTLRGRT
                     IINFFFEDSTRTRSSFEIAGKWMSADTINLSAKGSSTSKGESLRDSVQTINAMAVDAL
                     IIRHNQSGACHQVAGWVDASVINAGDGTHEHPTQALLDAYTLRSRIGDLDGKHVVIVG
                     DLTHSRVFRSNVITLKTLGARVTVVAPPTLMPAGIGSWSAADGFATSWDLDEVLEGDD
                     "
BASE COUNT         1562 a         3600 c         3526 g         1620 t
ORIGIN      
        1 tccccgtcct ggtgcatcgg gccaacgagc gaacggcccg gaggcgtgac cgacgccttg
       61 tgggacaagg tgcgtcccga ggcccaggct caggtcaagg cgtgtgccgc cgccggcccg
      121 aagatggatc tgaagcggtg tccgagatcg atggcgttga ctttcgccct ttcgcgcatc
      181 ggcgtccgcc tgctcgccga gccgacccat tgagccacgg tagaatcgtc accgatccct
      241 gaccatctgc gtcggtgacg cgcgcgctcg cgtgcgccgg cggcatacga aagagactcc
      301 gtggcgacca ccaacgacct caagaacggc ctcgtgctca atatcgacgg gcagctgtgg
      361 accgtcatcg agttccagca cgtcaagccc ggcaagggcc cggcgttcgt gcgcaccaag
      421 ctcaagaacg tcctctcggg caaggtcgtc gacaagacct tcaatgccgg cgtcaaggtc
      481 gagacggcga acgtcgacaa gcgcacgatg cagtacctct acaacgacgg ctccgacttc
      541 gtcttcatgg accccgacac ctacgaccag atccacgtcg gccccgacgt ggtcggtgac
      601 gcggcgaagt acatgctgga gaaccaggac gcgatcgtgg ctcgccacga cggcagcgtc
      661 ctttacatcg agcttcccgc ctccgtcgtc ctggagatct cctacaccga gcccggcctg
      721 cagggcgacc ggtccaccgg cggcaccaag ccggccaccc tcgagaccgg cgccgagatc
      781 caggtcccgc tcttcctgga ggccggcacc aaggtcaagg tggacacccg tgacgggtcc
      841 tacctcggcc gggtcaactg acctgcgaga caactgatcc cggatggcgg ctcgcaccaa
      901 agcccgtaag cgtgccctcg acctgctctt cgaggccgag gcccgcggcg tcaacgtcgg
      961 cgacctgctc gccgagcggc tcgacgcgcc ggtgaccgag gcgcccctca atccgtatac
     1021 ggcgaccctg gtcgagggtg tcgtcgcgca ttggcgcgac atcaacgagc tgcttaatac
     1081 ctacagccag ggctggccgg tcgagcggat gcctgcggtc gaccgcgcca tcctgcggat
     1141 cggggcgtat gaggtgctct acagcgacga ggtcccggac aaggtcgcca tcgccgaggc
     1201 cgtagcgctc gccaaggacc tctcgaccga cgaatcgccc aagttcgtca acgggctact
     1261 agcccgactg ctgcaggtca agcccactct ggcctgagtc gggcttctcc aggtccgggc
     1321 cggcccggcc ggaccgacca aaccgctgcc gtccccgcgc ggacctgaga cgatcggggt
     1381 atgcggattc gctcgccggc cgtcgacggg gtcacggtgt ttgccgtcgt cggcgtcaac
     1441 acggtgtcct tcggtctgcg cgtcagcgcg ggtgcccgca aggggctgct cgggttcgcc
     1501 gtccagcggc gcagcgccgg tggccggtgg cgctatgtcg aaggcttcaa ggtcttccgg
     1561 tcgctgactc ccgaccccga gcccggcgcg acccattcga cccgccgcca cccgatccag
     1621 tcactcgtct gggacgattt caccgtgcgg gagaacggat cctacgacta ccgcttcatc
     1681 cctttccgcg gcaccccggc cgaaccccgg tatggcacgc cggtcgagat cacggtgcgc
     1741 agcgagccgc tgtggggtga gcggcatacc atcgtcttca atcgcggagt ggccagctcg
     1801 caggcgtacc aaagccgttt cggcgggctg gccccggacg agcagcccac tgccgccgcc
     1861 cggcagctcg ccctggactg gctcagccgc gacctcgacg agaccttcct cgccgtgatc
     1921 cggggggccc agcccggcga cgccctgcgc ggcgcgttct acgaattcac ctacgcgcca
     1981 gtgctttccg aattcgcggc tgccatcgag cggggcgtcg acgtgcgact ggtcctcgac
     2041 ctgaaggtga acgagcactg ggagaacgtc cgcacccccg acgggacgac cacccgcacc
     2101 tgggtcgaga gcgccccgcg agaggcgagc ctgcgggcgt tgtccgacgc cggcataccc
     2161 gcctctgccg tggttgcccg gcaggcacgg gcaaatgacc tgcagcacaa caagtttctc
     2221 gtccacatcc gcaagggcga gccggctgcc gtctggaccg ggtcgaccaa tgtcacgctc
     2281 gggggcatcc acggccagtc caacgtcggc caccacgtgc gcgatccgga gttggcggcg
     2341 gggttcctcg cctactggga gatcctcgcc gcggaccccg gcgcacgggc gggcatgacc
     2401 cgcggcgagg tcatccgcgc caaccgggag ttcgaggccg cggtggacgc gctcagcgcg
     2461 gttcccggct cgcgctcgtc cattcccacc ggagtgagcg cgatcttcag tccgcgctcg
     2521 accccagccg ccctggaact gtatgccgat ctgctggccc accccgaccg gctcgcctgt
     2581 gccaccttcg ccttcggaat cgccaaggaa cttcgggccg tgctcgagca aggcgacgag
     2641 gactctccgc tgcgcttctt cctcctcgaa aagcgcactc tgtcaaccca atccacctcc
     2701 tccgcagcga cggtcgagct ggactggcgg cgcaatatct atttggcctg gggcagtgag
     2761 ctgggcacgc cgctcgggca gtgggtcgcc gagaccacga cccgcgaact cggactgaac
     2821 cggcatgtcg ccttcattca cgacaaattc ctgctcggcg acccgctcgg cgccgacccc
     2881 gtcgtcgtga ccgggtccgc caacttctcg ctcgcctcgt cggtggacaa cgacgagaac
     2941 atgctgctga tccgcggcga cacccgcgtc gccgatatct acttcaccga gttcaaccgc
     3001 ctcttctttc actactactt ccgctctgtc gtcgacagcc tctccggtcg cgaccgcgct
     3061 ggcgcccggc gatcggcccc ttccgcgctc gacctcgtcg aggacgacag ctggctggcg
     3121 aagtatgcac ccggcaccct gcgcaccaag cgcgccgagc agttcgtgcg gatggccggc
     3181 gcgcagttgt ctgcggcgac atccgagacg tgaatcgtga gtgcgggtca gcttctccag
     3241 atttcgacgt gccctcggtc gttcggtgcg agccacgtgg gaattgcggc atctgggcga
     3301 ccggtggtgc ggatgtgctg gtcccacaag agggccaaga gagcattgac gtccgggagc
     3361 cgggtcttct tcgcagcggg tgaggcgacc gccttggcgt cagtcagcgt cgcggaaggc
     3421 agccaccagg tcgtcggtca cgacatgacc cgggacgccc cgcagcacag gaattccgtc
     3481 gccggccgac gccggcccgt ccgtggcggt cgggagcgca agcccagcta gggcgatatc
     3541 cgagacggct cgccccaggg aaataccctc ggctcgggct gtggcgcgtg cggcgctcag
     3601 gaccacgtca tcaagctcca aggtcgtgcg catcagcaca tcacagcatc accgcttcaa
     3661 tgattgttgg tgcctgatca gcctgggctg ccttgcccct tagggttggc ggcgtgccag
     3721 cccggctgag tttcaccgcg atccggtatg ccgacgcggc cgatatcccg ctgatcgagg
     3781 gcatcgagcg ggccggggat acccgtttcg ccgaggtctc ccgagcgcgc ggtatcgatc
     3841 ccgggctctt ccccggggtg ggtatgccgg ggcccgagcg cgcggcttcg ccgggtttgg
     3901 tactggtcgc cgggcagccg gcggtcggct tcgcgcacat cgttccggtc gcgggctgcc
     3961 ttgtgctgga acagctttcg gtcagtcccg acgctgggcg gcggggagtt ggcgggtcgc
     4021 tcctgcacgc ggccatgggc gcggccctgg acatgggcca cgacacgctc tatctgcgga
     4081 ccttcgccga ggtcccctgg aatgcgccct tctatgcccg gagcggattc ctcgagatcc
     4141 ccgatcccga gtggatcgct ccgctggtgg cggccgagga cggtgcgggc ctgcgccggt
     4201 ggggtcggcg aatcacgatg gcctgcccgc tcgccgacga gccggtgccg cggccggcgg
     4261 tgagcgtcat accactcaag gacggccccc gcggtctgga agccttcgtc caatatcgcg
     4321 tctccaccat ggacttcgcg gccggcgccg tcgtcttccc cggcgggcgg gtggatccgg
     4381 tcgatcacga tgccgccatc acgctgcccc agacgacact ggacgagcag gcagaccgtt
     4441 gggccgcaac ggatttccgc aaactcggcg cccggccggg cgatgcggcg cgcacgatcc
     4501 tcgccaccgg agtgcgggaa gtgcgcgagg aggccgcggc cgtcatcgac ccggcgaccc
     4561 tgatcccgtg ggacaactgg gtgacaccga cgggcatctc ccggcgattc gacgtctact
     4621 tcctcatcgc cccggtgaac gaaggggcgg cgggggagtg gcggcatacc accagcgagg
     4681 cccacgactc gcactggacg ccggtagagg cgctcgccga gcgcgccgaa cgcggtgagc
     4741 tgctcctgct gccgccgacc cgcgcgattg tcgacgagct cctcgctctc ggtgacctga
     4801 gcagcgtcct cgcgctgacg ccggcgatcc gggcggtgcg ccacgatatc gacatccggc
     4861 gcccccgagg ggggtgactg gccgggtcga gccgcgctag gttgctcggc catgggagcc
     4921 aaccacaccg catacccacg acgcgcgcag cgcgccaccg ccgccctcgc cgccctcgcc
     4981 ctcaccggcc tcgtggtgag cgtcagcgct cccggggccg cctccgcgga ctccggcgag
     5041 ccgaccatga tgatgcccac gggcggcggc tatgagccgg tggccctgca ggccttcggc
     5101 aaggccgcga tctcgaaggc cgacgacgcg acggtcgaca tcgtcgtcgt cccgtccgcc
     5161 tatggcgacg acctgccgag ccggccggag aatatcgccc tggcccggga gcggaccgcc
     5221 gaaatcgccg ccgcctgcaa cgaggtggtc accgggacgg gcaagcggtg tgtcggccgg
     5281 ctcgcgatcc tgctcaaccg cgcagacgcc ctgaacgccg ccaactccgc cgccctgcgc
     5341 aatccgagcc tggacggcat ctacatcctc ggcggcgacc aaggcatcgc gatgaaggtg
     5401 ctggccaact cgccggccga gcgagccatg gcggcggccg tgacgggtgg ccgtgtggtg
     5461 ctcgccggca cgagcgccgg agcggccgtc gaatcccggt ccatgatcaa cggctatgtc
     5521 ggcgattacg gtccagcaca aggacttcgg cgcggcagca cgctgatgtg gtggggcgac
     5581 gacagcgatg ccgagcgcgg cctggcgttc gggtcccgca aggcgatcta cgaccagcac
     5641 ttctatcaac ggggccgctt cggacggtcg atgtcgacga tcgccaccgc tgacgagcgc
     5701 ttcggcggca ccagccccgt cggcgtcggc gccgactacg ccacgggtgt cgtcaattac
     5761 ggcgacaagc tgctgaccca ggtcttcggc gacagcggcg tcgggacgat cgacttcgag
     5821 tctctgggcg cgacccaccg gtgggtcggc acggagcgct ggctgtcggc gcgcaaggtc
     5881 aaggtcaacc tgatgaccga aggcaccgac ttcgatctcg acagccggcg cctgacccga
     5941 aatggggcgc tcgtgggcca ggtgagcggg cgggcgtgga gtgcccccgc cgcccccggt
     6001 cgcggagtgc tctacctcgg cggcgggatc ctgcagacca ccaaggtcat gggatcggtg
     6061 gtcaaggatg ccgcgtccgc gagccccgct ccggccgacg cccgcctgct catcctgtcg
     6121 gcggactcat cctcgacggc agcggactat ggcgccctcg ccaagcgggc gggatggatg
     6181 ggccacatcg acacggcggt ctatggcagc gcgtcctggg cgggcgtgga catcggccgg
     6241 tatgcggcgg tgctggtcgt cgcggtcgcc ccgccgaagt cggttgccgc cttcgccgac
     6301 gcgcgcttcc gtggcctggt caccacggcg gtggccacgg cgcccgtcgt gttcgcggac
     6361 ggtcccgccg cggcatacct cgggtcgcgc tggtccgcgc aggcgcggcc gaacggcgac
     6421 gattatgagg acaaggcggt ggcggccttc aagaccaccg aggccaaatg gcgcgccggc
     6481 accggttcag tcgcggcgac ggtgatcccc gcgatcaaca ccgactacca gtggggccga
     6541 gcgttcaacg ccgtcgccgt cgccaagggc caacttgccc tcggcgtgag tgcaggcgcg
     6601 gccatccgcc tgcagggcgg agcggctcgg gtcgtggacg gctcggtcgt cgtggtggac
     6661 ggccgcgatg ccaccacctg ggcgtcgagc aatggcaccc tcggggcggc cgggctggtg
     6721 ctcgacacct tcggcccggg ggaggcgctc cgccggtagc cgcgccgcct tggcgttaag
     6781 gaagctttgg gcgggaacct ggcggtgcgc agcgcgaatg ttccgttgcg caccgccagt
     6841 tttccgccct gggcggaaaa gtgcgatccc acagagaggg cgtcagcgct tgccggagaa
     6901 ggcccaggcg gtgacgttgg cggtgtcctc gcggccgaag aagggctcgt tgagggagta
     6961 gcggtcggtg gtgaagtcgg tgtcaccgtc gtcggcggtg ccgagcaccc agtcggggcc
     7021 ggcgttgaac atccgccaga agccgcggcc gccggagtcc atgatcgact tcacggagtt
     7081 ggagttgtcg gtgtgccagt tgccgatgta gtggccgact tcgtgcgcga ccacattgcc
     7141 gacggcccgc ccgatcagct gcaccttgtt gccgacaccg ggccgcaggt agtagttcag
     7201 cgacaccacg gcggacgggt tgcccttgac cggggcgctc atcaggtcct gaaggacgag
     7261 cgcggtgtcc tcgcggccgt aattgcccgg gtcgatgtgc tcggcgatgc ccacggtcgg
     7321 aatctgcgac tcgtcgatgg tcccaccgac gatgacccgc gagacgttcg gccggccgaa
     7381 ggtgtcgggg gaggtgcggc cgttgacgat gcgcacgttg aggttcggga tgttcttgcg
     7441 gcgcatgtca tcgcgcaggt tctccgtgac cgtcttggtg atcgcgtccg tgagggcagg
     7501 aaggtcggcc tgggtcaggc cccagccctt gaggaaggag gacaagggcg agagcttggc
     7561 ctgcatgtcg gggccgccga atatcgaggt gttgatgctc gcgccgtcga agtcgagata
     7621 gatcgtctgc ggggccgacg tggactcccc gccgggacgg tagacctcga cagcgttgcg
     7681 gtaggcgccg atccccgttt ccgtgatcac gccataccag ccgtcggcct ccgcgacata
     7741 ggccgtcgcg gcattgccgc cgccaggcag tggggagttg ggcgggtagt aggccgtcgt
     7801 gtcactctga ctgcttgccg tgacataggg cgagtcccac ttctggacgg atacgcgccc
     7861 ggcggctccg gtcaccgagg tgccgagcac atccccgcgc cggagcttga ccaggaccgc
     7921 gtcccggtcg gtccggtagg cgccgaactc ggccgtgtaa ttgccggagt tggctgctcc
     7981 cgtgccggac gccgagtcgg agaaatccat ctgccatgtg tcggggtcga ggaggaggag
     8041 gtagtaggtg ccgccggccg tactggtggt cgatgccgtc ttgtcgggca agctgccatc
     8101 gggggtgtgc caggcgacat cgagcactgt gccgtgcccg tccacgagga gtccgaacac
     8161 gtccaaggag gaactgggta tggcggcgcc caccgtctcc ccgggcttgg cggtgagctt
     8221 gtaccagtcg aagtcgcccg tgctcgtgcc atagggcccg tcgccgatgc gcccggtgat
     8281 cttcacccgg ccgttcccgt cgatgcccgt cgcggcggcc ttggggatcg atccgttgtc
     8341 ctccttggcc atcgggagcg atcggagggc ggggcgcttg tccgcgagcg tgccggagac
     8401 gaccatgcgg ttgtgcagcc cggcgccgag gccgaacgcg gagaccttct cacccttcca
     8461 cgtcgtgtcg ttggccccga tgacggtggc cgcttccttc tcgcggtagc tcaggacggc
     8521 ccggtccgag gcgccggacc acctcgcctt ggcggcctgg cgctcgggtg tcgtggccgc
     8581 ggcggcagag atcttcgcga ccttggccac ccaggcggcg aagttggtct tgtgcagatc
     8641 gggcagataa gcggtgaacg ggctgggccc ggtggcgccg gagcgcagcg gcgccagttg
     8701 cacccccgcg tccagtcggg ccgactcggc ggcgctcagg ccttcggaac ggtccggact
     8761 cccggcgttc ccggcactcg ccgcacccgg gactacgagc gcgatcaatc cggcacccgc
     8821 acacacgagc attcctgcgc gcttcattgc tttccccttc ccctgcattg cctgaagctg
     8881 ggggagcatc cccacgaccg actctagggg cctgtgtcca gccgcgctac gggggggtat
     8941 gccgtggtga ggggacacgg catacggacc gctggcgccg ggttggccgg ggaggggtta
     9001 cagtggcccc gtccgcatcc tttaacgctg tcccgtgagg cagggaagga ggcgcgcgtc
     9061 atgacgcgcc ctgacagcag tggccctccg gccgggtccc ccgtcgaccc tcacggccga
     9121 accgtcctct ccagcaacga gatcgctcgc ggcctgcgcc gtatcgcgca cgagatcctc
     9181 gagcacaaca agggcgcggc cgatctggtg ctgctgggga tccccagccg cggcgtcgaa
     9241 ctcgccgagc ggctcgcgct catcctcgcc gaggtggagg gctgcgaccc cctcccggtc
     9301 ggctcgctgg acatcacgat gcaccgcgac gatctgcggc accagccgac gcgctcgccg
     9361 atgcgcacgg tgatcccgga ctgcggggtg gacgacaaga tcgtcgtcct ggtcgacgat
     9421 gtgctctatt cgggccggac catccgggcc gcgctggacg cgctcgccga cctcggccgg
     9481 ccccgcgcgg tccggctggc ggtgctcgtc gaccgtggac accgggaact cccgatccgc
     9541 gccgaccatg tgggcaagaa cctgccgacc tcgcacagcg agaaggtgca ggtgcgcctg
     9601 cgcggccacg acggcgtcga ggacgtcgtg cggatctcgg gagggaactg accgtgccca
     9661 agcacctgat cagcagcgcc gacctggacc gcggtgaggt cgaggccatc ctcgagaccg
     9721 cgtcctcgat gcacgacgtg cagcgccggg aagtcaagaa gctgccgacc ctgcgggggc
     9781 ggacgatcat caacttcttc ttcgaggact ccacccgcac ccgcagcagc ttcgagatcg
     9841 ccggcaagtg gatgtccgcc gacaccatca acctctctgc gaaaggctcc tccacctcca
     9901 agggggagtc gttgcgcgac agcgtccaga ccatcaacgc catggccgtc gatgccctga
     9961 tcatccggca caaccagtcc ggcgcctgcc accaggtggc cggctgggtc gatgcctcgg
    10021 tcatcaacgc cggcgacggc acgcacgagc accccaccca ggcgctgctg gacgcctaca
    10081 ccctgcgctc gcgcatcggc gacctcgacg gcaagcatgt ggtcatcgtc ggcgacctca
    10141 ctcacagccg ggtcttccgc agcaatgtca tcacgttgaa gaccctcggg gcccgggtca
    10201 ccgtcgtggc tccgccgacc ctcatgccgg cgggcatcgg atcctggtcc gccgcagacg
    10261 gattcgccac atcctgggat ctcgacgagg ttctggaagg cgacgacg
//
LOCUS       DAOPJG010000121        10243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6868_length_10243_cov_6.640165, whole genome shotgun sequence.
ACCESSION   DAOPJG010000121 DAOPJG010000000
VERSION     DAOPJG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6868_length_10243_cov_6.640165"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PKU75_11210"
     CDS             complement(<1..1047)
                     /locus_tag="PKU75_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPF81493.1"
                     /translation="MRPDVVVRTLTARGLLGLELPHRRARQLLALQRWGYGIAGELRA
                     AAAVSGSRVALIDERGAHTYSEAVDLGDRLARVLRSRGLPAGARVGLLAPNGVEFVLA
                     LYAANLLGIDLVLLNTGLTGERITQIADRDDLQTVLHDGELAHLVGGVAAAYDLGELM
                     TQARDAAYAPSGGRLAPARAEGRTIVLTSGTTGVPKGAPRRAVGGIGAIVSILERIPL
                     HHRDRILLSAPLFHTWGFAGLQLAFGVQATVILQRRFEPARAQAALAEHRAQVLFAVP
                     AMLQRLLAWREAVPEAGMPHLRVVASSGSKFPPGFATRFLDEFGEVLYSLYGSTEVSW
                     ATIATPEHLRRDPDT"
     gene            complement(1058..3811)
                     /gene="topA"
                     /locus_tag="PKU75_11215"
     CDS             complement(1058..3811)
                     /gene="topA"
                     /locus_tag="PKU75_11215"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPF81494.1"
                     /translation="MLAGRKLVIVESPGKIKNISAYLGPEYDIEASFGHVRDIPTPSE
                     MPADMKKGPFGKFGIDVDNGFEPYYVVDPDKKKRVSDLKKLLKNADELYLATDDDREG
                     EAIAWHLYEALQPKVPVKRMVFTEITREAIQRAMANTRQIDENLVEAQETRRILDRLY
                     GYEVSPVLWRKVRAGLSAGRVQSVATRLVVERERERMAFRAASYWDVEADLATDAGES
                     FTARLTAVDGTRVATGRDFGDDGALRGSALHLDQAAAQGIAEALRTAPARVANVADKP
                     YKRRPYAPFITSTLQQEASRKLRLSARETMRIAQRLYEGGYITYMRTDSVMLSETALN
                     AARSQARSMYGAEYVPDAPRRYTSSNASAQEAHEAIRPAGDTFRTPSQVSSQLRGSEY
                     ALYDLIWKRTIASQMADAVGSTATVQTEAALADGRVAQLSASGTVITFRGFLAAYEEG
                     RDEEDTRARQAAREAGEEERRLPQLSQGDALDVHRTEASGHETSPPPRYTEATLVKAL
                     EQKEIGRPSTYEPTLATIQDRGYVIKQGSALVPSWLAFAVTRLMEEHFGRLVDYDFTA
                     SMEDDLDAIARGDAQRVEWLQRFYFGDEATSAEGLRDLVADLGEIDARGISTIDIGDG
                     LVVRVGRYGPYVEEVSPAGVDPSTGEMADDAATTPRRATINDDVAPADLTPEMARALL
                     EVSADDGRSLGTDPATGHEILAKAGRYGPYVTEVLPESDAPASRTRKAAVAKPRTASL
                     FKDMDLGTIDLDTALQLLSLPRIVGAHPETGAEITAQNGRYGPYLKMGTDSRSLETEA
                     QLFDISLEQALAIYAQPKQRGRAAAKPPLKELGNDPVNGKPVVVKDGRFGAYVTDGET
                     NATLRRDDDPETITPERGFELLAEKRAKGPSTRRGAKKTTKKTTKKSAKKTTRKTAAK
                     KSD"
     gene            3954..4736
                     /locus_tag="PKU75_11220"
     CDS             3954..4736
                     /locus_tag="PKU75_11220"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HPF81495.1"
                     /translation="MTDRAPILYLSHGAPPLADDARWTQELAHWSAGIARPDSILMVS
                     AHWEEAPVTVSASAAPASLLYDFWGFPQKYYEVRYDAPLAPALADDVSRLLGGQVHRD
                     ESRGLDHGAYVPLVEMYPEADVPVLQLSLPTLDPRALFELGRALAPLRDTGTMIVGSG
                     FTTHNLRWFDPGAGADGPAPAASAEFDNWAAEAMASEDLDALLDFGHRAPAARLAHPR
                     TEHWAPLYVALGAAYQSGGIANHSVIDGFWFGLSKRSWEFAA"
     gene            4827..6068
                     /locus_tag="PKU75_11225"
     CDS             4827..6068
                     /locus_tag="PKU75_11225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPF81496.1"
                     /translation="MRGRRVLAMAVVAAVGGGLTSCADDGAESTSAARGGSSAQQTGS
                     MSSPSTAESPSKSSKSGASAGRVTIGASGDLLTHVAVRRSAEAYAGSAGHYDFRPMFA
                     KVAPLIAKSDISICHMETPLTSTNTNLSQPGVLVFNTPHELADAVKDAGYDGCDFASN
                     HTWDRGLAGLQDTIKVFDTAGVKLAAPGGSATTPRLVTTYDVNGVKVAHLAYTYTIYN
                     DWGPNTKVPPEAPWLGESLWPAVNSEGIIRDAKQAKADGADFVVVSMHWGQEYVDDPT
                     EDQTRVATALLQSPDVDLILGTHVHRVQPCSVINGKYVFYGLGNFLSNQSPSVDASLI
                     PQTQEGVHVTVTLERDASGKVISSATYQPTKVNLAGHVITPATASSNPTTFDRVTKTL
                     QMLGNCPLDGKVATSHPSSLE"
     gene            complement(6107..7594)
                     /locus_tag="PKU75_11230"
     CDS             complement(6107..7594)
                     /locus_tag="PKU75_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF81497.1"
                     /translation="MLSSDPALIAALRADLSAAPFTVERTAAVLGPVAAGALAREQVL
                     PAERATRDNPDPAAVLIRLFTLGDPVDRATVDAALPALGGAGALALGLVRDAGDALIA
                     TCDLRPYGDQEHAWWLASDLSELATRAPLSEDHVLGVGGASTTLASWTPRPRVGRALD
                     LGTGCGVQALHLSTHAEHIVATDISDRALSYVRFNAALNGQEWDIRHGSMLEPVAGER
                     FSLIVSNPPFVITPRDHGMPTYEYRDGGQQGDAVVADLVTRLGEHLEPGGIAQILGNW
                     EIVGAAPWQDRVGAWARAAGVDVWVVQREIQDPAQYAETWARDGGHQSGTTDFNQMYA
                     AWLDDFAARDVTGIGFGIITMHRPESDREPFVDLLDARGPVAAPMGPAVLAGIQARVW
                     LAEHDDDAILDTSWSAAADVTVAQHFRAGSSDPEVIEVAQGGGLGYRESLDTASAAYL
                     SVADGDLTARAALAAISGLLGLDQDELVEAMLPRLRRWIAAAILR"
     gene            complement(7742..8626)
                     /locus_tag="PKU75_11235"
     CDS             complement(7742..8626)
                     /locus_tag="PKU75_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81498.1"
                     /translation="MPAMNPVIARARTRLVVALAALAVLAPAALAAAQYPRYWEWIAL
                     ELTPMTWLQTVLLVLAAATSAVIAVIGKVHGWSRALRIPHWLLAAGFAYLALDDRFAI
                     HERIRDRILAPRDISIPGLSFLAPGDFQVLLMGIAGLIVLPFVLRALRRDRLALWLFV
                     LGVVCSVTSIGMDAIDPATMPVDVERLEQTLEECVELLAAAFYVAALGVRLLGLLDPA
                     TSTATSSATSTQMAAQTPEYAAVPAAAGTVPAAADAGPDSRMPTGHDPRRGPGEDGVY
                     APQAERTEELLTTRAGRP"
     gene            complement(8631..9632)
                     /locus_tag="PKU75_11240"
     CDS             complement(8631..9632)
                     /locus_tag="PKU75_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF81499.1"
                     /translation="MSQIVVLSRAGEASLPPASVKALRVRHGVRFLQRHDAPTGEEAA
                     ELLAGATVLASTNATLPVLDDALLDRLPGLRHVVLYATGYEHLDIEGLAGRGITVTTL
                     PEYATNAVAEHALALIFACATRIHLANDKARGRAPHHVSLRGVEISNRTLAIIGMGRI
                     GRRLAQLAGGLGMNIIGVDIDPDAVASAAAARIRMVTHEAALREADLVALTASTIEGH
                     FPILGARELALLHPDAFVVNVGRPVLADTDAVRDRLLARRLRGYAVDEVALDPGDPID
                     AVLMAEGRVLQSAHSAWWRDEVLARGAQMFAQAIQAAADGAPIHVVGPLDHALAAAR"
     gene            complement(9799..>10243)
                     /locus_tag="PKU75_11245"
     CDS             complement(9799..>10243)
                     /locus_tag="PKU75_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPF81500.1"
                     /translation="ANSGGAWDNAKKIVEYGRFGGKGTDAHAATVIGDTVGDPFKDTA
                     GPAINPLIKVMNLVSVLIAPAIVTLTIGNSANAGIRYGIAGAALLIVVAAVTVSKRRS
                     ISIGGDDAPAVDATPAAHAPAPVGSRSDLAEHTAVQVEERDVDPR"
BASE COUNT         1457 a         3515 c         3739 g         1532 t
ORIGIN      
        1 ggtgtcgggg tcgcgccgga ggtgttcggg cgtcgcgatg gtcgcccagg acacctccgt
       61 cgagccgtac aggctgtaga gcacctcacc gaactcgtcg aggaagcgcg tcgcgaagcc
      121 cggcgggaac ttcgagccgc tggaggcgac cacccgcagg tgcggcatcc cggcctccgg
      181 gacggcttcc cgccaggcga gcaggcgttg cagcatcgcc ggcacggcga acagcacctg
      241 ggcccggtgc tcggcgagcg ccgcctgggc gcgggccggc tcgaacctgc gttgcaggat
      301 cacagtggcc tgcactccga acgccagctg cagtccggcg aacccccacg tatggaagag
      361 cggcgcgctg agcaggatcc ggtcccggtg gtggagcggg atgcgctcga ggatcgagac
      421 gatggccccg atgccgccca cagcgcgccg cggggcgccc ttgggcaccc cggtggtccc
      481 gctcgtcagg acgatggtgc gcccttccgc ccgcgccgga gcgagccggc cgcccgacgg
      541 ggcgtatgcc gcgtcccgcg cctgcgtcat cagctcaccc aggtcgtatg ccgccgccac
      601 cccgccgacc aggtgcgcca gctcgccgtc gtggagaact gtctgcaggt cgtcgcggtc
      661 ggcgatctgt gtgatccgtt cgcccgtcag cccggtgttg agcaggacga ggtcgatgcc
      721 gaggaggttc gcggcataca gcgccagcac gaactccacc ccgttcgggg cgagcagccc
      781 gacccgcgca ccggccggca gcccccggct gcgcagcacc cgcgccagcc ggtcgccgag
      841 gtcgacggcc tcgctgtagg tgtgggctcc ccgctcgtcg attagggcca cccgcgaccc
      901 ggagacggct gcggcagcgc gcaattcgcc cgcgatgccg tagccccacc gctgcagggc
      961 gagcaactgg cgagcccggc gatgcggcaa ttccagaccg agcaacccgc gcgccgtcag
     1021 cgtcctgacg acgacatcgg gccgcaccgc gaacacctca gtccgacttc ttcgccgctg
     1081 tcttgcgggt ggtcttcttg gccgacttct tggtggtctt cttggtggtc ttcttggcac
     1141 cgcggcgggt gctcggaccc ttggcccgct tctccgcgag cagctcgaag ccgcgctccg
     1201 gggtgatggt ctcggggtcg tcgtcgcggc gcagagtggc gttggtctcg ccgtcggtga
     1261 cataggcccc gaaacggccg tccttgacga ccaccggttt gccgttgacg gggtcgttgc
     1321 cgagttcctt cagcggtggc ttggcggcgg cgcgaccccg ctgcttgggc tgggcataga
     1381 tggccagcgc ctgctcgagg ctgatgtcga agagttgggc ctcggtctcg agggagcggg
     1441 aatcggtgcc catcttcaga tacggcccgt agcggccgtt ctgcgccgtg atctcggccc
     1501 cggtctcggg gtgcgcgccg acgatgcggg gcaggctgag cagctgcagc gcggtgtcga
     1561 ggtcgatggt cccgaggtcc atgtccttga acagggaggc ggtgcgcggc ttggccaccg
     1621 ccgccttgcg cgtgcgcgac gcgggggcgt ccgactcggg cagcacctcg gtgacatagg
     1681 gcccgtagcg gccggccttg gcgaggatct cgtggccggt ggccgggtcg gtgccgagcg
     1741 agcgcccgtc gtcggccgag acctcgagca gggcgcgcgc catctcgggg gtcaggtccg
     1801 ccggcgccac gtcgtcgttg atcgtggccc gccgcggcgt ggtcgcggcg tcgtctgcca
     1861 tctcacctgt cgacgggtcc acgccggccg gggagacctc ctcgacgtag ggtccgtagc
     1921 ggccgacgcg cacgacgagc ccgtcgccga tgtcgatcgt cgagatgccg cgggcgtcga
     1981 tctcccccag gtcggcgacc aggtcgcgca gaccctcggc ggacgtggcc tcgtcgccga
     2041 agtagaagcg ctgcagccac tcgacccgtt gtgcgtcgcc gcgggcgatg gcgtcgaggt
     2101 cgtcctccat cgaggcggtg aagtcgtagt cgacgagccg tccgaaatgc tcctccatca
     2161 accgggtgac ggcgaaggcg agccacgacg gcacgagcgc cgaaccctgc ttgatgacat
     2221 agccgcggtc ctggatcgtc gccagcgtgg gttcgtatgt cgaggggcgg ccgatctcct
     2281 tctgctccaa cgctttgacg agggtggcct cggtatagcg cgggggcggg gaggtctcgt
     2341 gaccggaggc ctcggtgcgg tggacgtcga gcgcgtcgcc ctggctcagc tgcggcaggc
     2401 gccgctcctc ctcgccggcc tcgcgggcgg cctggcgggc gcgggtgtcc tcctcgtcgc
     2461 ggccttcttc gtatgccgcg aggaagccgc ggaaggtgat caccgtgccg ctcgccgaca
     2521 gctgggcgac ccgcccgtcg gccagggcgg cctcggtctg cacggtcgcg gtcgagccga
     2581 cggcatcggc catctgggag gcgatggtgc gcttccagat caggtcgtag agggcgtact
     2641 cgctgccgcg cagctggctg ctgacctgcg acggggtgcg gaaggtgtcg ccggccgggc
     2701 ggatcgcctc gtgcgcttcc tgggcgctgg cattggagga ggtgtagcgg cgcggggcgt
     2761 cggggacata ttcggcgcca tacatgctcc gggcctggct gcgggcggcg ttcagggccg
     2821 tctcggagag catgaccgag tcggttcgca tataggtgat gtagccgccc tcgtagagcc
     2881 gctgcgcgat ccgcatcgtc tcgcgcgccg acagccgcag cttgcgcgag gcctcttgct
     2941 gcaacgtgga ggtgatgaac ggcgcatacg gccggcgctt gtagggcttg tccgcgacat
     3001 tggcgacccg cgccggggcg gtgcgtagcg cctcggcgat cccctgcgcc gcagcctggt
     3061 ccaggtgcag cgccgacccg cgcagcgcgc cgtcgtcgcc gaagtcgcgc ccggtcgcca
     3121 cgcgcgtgcc gtcgacggcg gtcaaccgcg cagtgaagct ctccccggcg tccgtggcaa
     3181 ggtcggcctc gacatcccag taggacgccg cccggaaggc catccgctcg cgctcgcgct
     3241 cgacgaccaa ccgggtcgcg acgctctgca cccggcccgc cgacaacccg gcccgcacct
     3301 tgcgccacag gacggggctg acctcatacc catagagccg gtcgaggatg cgccgggtct
     3361 cctgggcctc gacgaggttc tcgtcgatct ggcgggtgtt ggccatcgcc cgctgaatgg
     3421 cctcccgggt gatctcggtg aagaccatcc gcttgacggg gaccttgggc tgcagcgcct
     3481 cgtacaggtg ccaggcgatc gcctcgccct cgcgatcgtc atcggtggca agatagagct
     3541 cgtcggcgtt cttgagcagc ttcttcagat cggagacgcg cttcttcttg tccgggtcga
     3601 cgacgtaata gggctcgaat ccgttgtcga cgtcgatgcc gaacttgccg aaggggccct
     3661 tcttcatgtc cgcgggcatc tcggacgggg tcgggatgtc ccggacgtga ccgaaggagg
     3721 cctcgatgtc gtattccggc ccgaggtagg cggagatgtt cttgatcttc cccggagact
     3781 ccacgatgac gagcttgcgc ccggccaaca ccaccactca cctttccgtc accagcctcg
     3841 gccacggagg gccgaggcgg gcatatcggc gccaccgtat acgacgctcc cccgttttcg
     3901 tccatcgcac cgtttttgtt gaaaaataaa ttagttagcc gtatgctgct cgaatgaccg
     3961 atcgcgcccc aatcctctac ctctcgcacg gcgcgccgcc gctcgccgac gacgcccgct
     4021 ggacccagga actcgcgcac tggtcggccg ggatcgcccg gcccgactcc atactcatgg
     4081 tctccgctca ctgggaggag gcgccggtga ccgtcagcgc gagcgccgcg ccggcgtcgc
     4141 tgctctacga cttctggggc ttcccgcaga agtactacga ggtgcggtat gacgcgccgc
     4201 tcgcccccgc cctcgcggac gacgtcagcc gcctgctcgg tgggcaggtg caccgcgacg
     4261 agtcccgcgg cctcgatcac ggcgcctacg tcccactggt cgagatgtac cccgaggccg
     4321 acgtccccgt gttgcaactg tccctgccga cgctcgatcc gcgggcgctg ttcgagctcg
     4381 gccgggcgct ggcgccgctg cgcgacaccg ggacgatgat cgtcggctcg ggcttcacca
     4441 cccacaacct gcgctggttc gaccccggtg ccggcgccga cggcccagcc cccgccgcct
     4501 cggccgagtt cgacaactgg gccgcggagg cgatggcgag cgaggacctg gacgccctgc
     4561 tcgacttcgg ccaccgcgcc cccgccgccc ggctcgccca cccgcgcact gagcactggg
     4621 cgccgctgta cgtcgccctc ggcgcggcgt accaaagtgg gggcatcgcc aaccacagcg
     4681 tcatcgacgg cttctggttc gggctctcga agcggtcgtg ggagttcgcg gcctagcctc
     4741 cgcccgaagc agcccggccc gaggtggccg acaccacgag tgcgacctcc cacaggtgac
     4801 cagcacgcgc ccctagcatg ggtgacgtgc ggggtaggcg ggtcctggcg atggcggtcg
     4861 tggcagcggt gggcggcggc ctgacgtcct gcgccgacga cggggcggag tcgacgagcg
     4921 cggcacgcgg cgggtcgtcc gcccagcaga ccggatcgat gtcctcgcca tccacggccg
     4981 aatccccctc gaagtcgtca aaatccggcg cgagcgcggg ccgcgtgacg atcggggcga
     5041 gcggcgatct gctcacgcac gtggccgtgc gccgcagcgc cgaggcgtat gccgggtccg
     5101 ccggccacta tgacttccgg ccgatgttcg ccaaggtcgc gccgctgatc gccaagagcg
     5161 atatctccat ctgccacatg gagacgccgc tgacgtcgac caataccaat ctcagccagc
     5221 cgggggtgct ggtcttcaac accccgcacg aactcgccga tgccgtcaag gacgcgggct
     5281 atgacggctg cgacttcgcg tccaaccaca cgtgggaccg ggggctggcc gggctgcagg
     5341 acaccatcaa ggtcttcgac accgcgggcg tcaagctcgc cgcgccgggg ggatcggcca
     5401 cgacgccgcg cctggtgacg acctatgacg tcaacggcgt caaggtcgcc catctcgcct
     5461 acacctacac gatctacaac gactggggcc ccaacaccaa ggtgccgccc gaggcgccgt
     5521 ggctgggcga atccctttgg cccgcagtca attccgaggg catcatccgc gatgccaagc
     5581 aggccaaggc cgacggtgcc gacttcgtcg tcgtctcgat gcactggggc caggagtacg
     5641 tcgacgatcc caccgaggac cagacccgcg tcgcgacggc attgctgcaa tcccccgacg
     5701 tcgacctgat cctcggcacc cacgtgcacc gggtgcagcc gtgttcggtc atcaacggca
     5761 agtacgtctt ctacgggctg ggcaacttct tgtccaacca gtcgcccagt gtcgacgcct
     5821 cgctgatccc gcagacgcag gagggggttc atgtcacggt caccctcgag cgcgacgcct
     5881 ccggcaaggt catctcatcc gcgacctacc agccgaccaa ggtcaacctg gccgggcatg
     5941 tcatcacccc ggccacggcg agcagcaatc cgaccacctt cgaccgggtg accaagaccc
     6001 tccagatgct cggcaactgt ccgctcgacg gcaaggtggc cacgtcgcat ccgtcgtcgc
     6061 tggagtagcc cagtccgccg ttgacgatcg cgccagcggg gtcgcctcaa cgcaggatgg
     6121 cggccgcgat ccagcggcgc agacgcggca gcatcgcttc gacgagttcg tcctggtcga
     6181 gtccgaggag gccggagatg gcggccaggg ccgcccgggc cgtgaggtcg ccgtcggcga
     6241 cggagaggta tgccgcgctc gcggtgtcga ggctctcgcg gtagccgagc ccgccgccct
     6301 gggcgacctc gatgacctcg gggtcgctgc tgccggcccg gaagtgctgc gcgacagtga
     6361 catccgcggc tgccgaccac gaggtgtcga ggatcgcgtc gtcgtcgtgc tcggcgagcc
     6421 agacccgcgc ctggatgccg gcgaggacgg ccggtcccat cggagcggcc acgggcccac
     6481 gggcgtcgag caggtcgacg aacggctccc ggtcggactc gggccggtgc atggtgatga
     6541 tgccgaagcc gatgccggtg acgtcgcggg cggcgaaatc gtcgagccac gcggcataca
     6601 tctggttgaa atccgttgtc ccgctttggt gtccgccgtc gcgggcccag gtctcggcat
     6661 actgcgcagg gtcctggatc tcgcgctgca cgacccagac gtcgacgccc gcggcgcggg
     6721 cccacgcacc gacgcgatcc tgccacgggg cagcgccgac gatctcccag ttgccgagga
     6781 tttgggcgat gccgcccggt tcgaggtgct cgccgagccg ggtgacgagg tcggcgacga
     6841 cggcgtcgcc ctgctgaccg ccgtcgcggt attcgtaggt cggcatcccg tggtcgcgcg
     6901 gggtgatcac gaaggggggg ttgctcacga tgagggagaa gcgttctcct gcaacgggtt
     6961 cgagcatcga cccgtgacgg atgtcccact cctggccgtt gagcgcggcg ttgaagcgga
     7021 catagctcaa ggcccggtcg gagatgtcgg tggcgacgat gtgttcggcg tgggtgctca
     7081 ggtggagagc ctggacgccg catccggtgc ccaggtcgag ggcgcggccg acgcgaggcc
     7141 gtggggtcca ggaggccagc gtcgtggacg cgcctccgac gccgagcaca tggtcctccg
     7201 acagcggtgc ccgggtcgcc agttcggaca ggtcggaggc cagccaccag gcgtgctcct
     7261 ggtcgccgta cggccgcagg tcgcaggtcg cgatcaaggc atcgccggcg tcgcggacca
     7321 gcccgagagc cagcgcgccg gccccgccga gggcgggcag ggcagcgtcc acggtcgccc
     7381 ggtcgaccgg atcgccgagg gtgaacaagc ggatgagcac ggcagccggg tccgggttgt
     7441 cgcgcgttgc ccgctccgcg ggcagaacct gttcgcgcgc cagggcgccc gccgcgacgg
     7501 ggcccaggac cgccgcggtg cgctcgacgg tgaacggtgc cgcgctcagg tccgcgcgca
     7561 gggccgcgat gagggcggga tcggaactca gcacgcctca gcctagcggc gccgcgtctt
     7621 cgcccactca catcgagaga gcgcgacgcg cccatattcc agccggaaca tgggcgtgtc
     7681 gcgctctctc gactggtggc gactgcgcgc cggggtgtgg tcgggcggtg tggtcgggcg
     7741 gtcagggccg gccggcgcgg gtggtcagga gttcttcggt gcgctcggcc tgcggcgcgt
     7801 agacgccgtc ctcgcctggc ccgcggcgcg ggtcgtggcc cgtcggcatg cgcgagtccg
     7861 ggccggcgtc ggcagcggcc gggacggttc cggcagcggc cgggacggcg gcatactccg
     7921 gcgtctgggc agccatctgc gtggacgtgg cgctggacgt tgcagtggac gtcgcggggt
     7981 cgagcaggcc gaggaggcgc accccgagag cggcgacata gaaggcggcg gcgagcagtt
     8041 cgacgcactc ctcgagggtc tgctcgaggc gttcgacgtc gacgggcatg gtggccgggt
     8101 cgatggcgtc catgccgatc gaggtgaccg agcagaccac acccaggacg aacagccaca
     8161 gggccagccg gtcgcggcgc agggcgcgca acacgaaggg cagcacgatc aggccggcga
     8221 tgcccatgag cagcacctgg aagtcgccgg gggcgaggaa gctcaggccg gggatggaga
     8281 tgtcgcgcgg ggccaggatg cggtcgcgga tgcgttcgtg gatcgcgaac cggtcatcga
     8341 gcgcgagata ggcgaagccg gccgcgagca gccagtgcgg gatgcgcagg gcgcgcgacc
     8401 agccgtggac cttgccgatc acggcgatga cggccgaggt ggccgccgcg agcacgagca
     8461 gcacggtctg caaccaagtc atcggcgtca gttccagcgc gatccactcc caatagcgcg
     8521 ggtactgcgc tgcggcgagg gcggcgggcg cgaggacggc gagggccgcc agggcgacca
     8581 cgaggcgggt ccgggcgcgg gcgatcacgg ggttcattgc cggcatctcc ctagcgtgcc
     8641 gcagcgaggg cgtggtcgag gggacccacg acgtggatgg gcgcgccgtc ggcggcggcc
     8701 tggatcgcct gggcgaacat ctgcgctccc cgggcgagga cctcatcgcg ccaccacgcg
     8761 ctgtgcgcgc tctgcagcac ccggccctcg gccatcagga cggcgtcgat cgggtcgccg
     8821 ggatcgaggg caacctcgtc gacggcatac ccgcgcagcc ggcgggccaa gaggcggtcg
     8881 cggaccgcgt cggtgtcagc gagcacgggg cggccgacgt tgacgacgaa ggcgtcgggg
     8941 tggaggagag cgagttcgcg ggcgcccagg atcgggaagt ggccctcgat cgtcgaggcc
     9001 gtcagggcga cgagatccgc ctcgcgcagt gccgcttcgt gggtgaccat gcggatccgg
     9061 gcggccgctg cggaggcgac cgcgtcgggg tcgatgtcca caccgatgat gttcatgccg
     9121 aggccgccgg cgagctgggc gagccggcgc ccgatccggc ccatgccgat gatcgcgagc
     9181 gtccggttgc tgatctcgac cccgcgcaga gagacatggt ggggggcgcg accgcgggcc
     9241 ttgtcgttgg ccaggtggat ccgggtggcg caagcgaaga tcagtgcgag ggcgtgctcg
     9301 gcgaccgcat tggtcgcgta ctccgggagc gtggtgacgg taatgccgcg gccggcgaga
     9361 ccctcgatgt ccaggtgctc gtagccggtc gcgtagagca cgacgtgacg caggccgggg
     9421 agccggtcca acagggcgtc gtcgaggacg gggagggtgg cattggtcga ggcgagcacc
     9481 gtggcgccgg cgagcagttc ggcagcctcc tcccccgtcg gtgcgtcatg ccgttggagg
     9541 aaacgcaccc catgccgcac ccgcagcgcc ttgacgctgg caggcggcaa gctcgcctcc
     9601 ccggcccggc tcaacaccac gatctgcgac atcgcactca ccctccgtcg ttcggctgcg
     9661 ggtccctccc gccgttgttc ccccagacgt gggaaccccg aatcaggttg tcgtgctggc
     9721 gaaaataggc ccggcgtggg ctgcctcgga ggcagcccac gccgggcctg gcgggatcgt
     9781 tatcggtcgt tgacctgatc agcgggggtc gacgtcgcgt tcctcgacct gcacggccgt
     9841 gtgctcggcg agatccgagc ggctgccgac cggggccggg gcatgagccg ccggggtggc
     9901 gtccacggcc ggcgcgtcgt ccccgccgat ggagatggac cggcgcttgg agaccgtgac
     9961 cgcggcgacg acgatcagca gggcggcgcc ggcgatgccg tagcggatgc ccgcattggc
    10021 gctgttgccg atcgtcaagg tcacgatggc cggagcgatg agcaccgaga cgaggttcat
    10081 caccttgatg agcgggttga tcgccggacc ggcggtgtcc ttgaaggggt caccgacggt
    10141 gtcgccgatg acggtggcgg cgtgggcgtc ggtgcccttg ccaccgaacc ggccgtactc
    10201 gacgatcttc ttggcgttgt cccaagcgcc gccggagttg gcg
//
LOCUS       DAOPJG010000122        10206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6897_length_10206_cov_5.915279, whole genome shotgun sequence.
ACCESSION   DAOPJG010000122 DAOPJG010000000
VERSION     DAOPJG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6897_length_10206_cov_5.915279"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="cysC"
                     /locus_tag="PKU75_11250"
     CDS             complement(<1..1001)
                     /gene="cysC"
                     /locus_tag="PKU75_11250"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HPF81501.1"
                     /translation="MSVHLPAAIAEQAASAGAVEIVDPEGVPLARLAATGETVHDGCL
                     EVEAEPAWLSSPSPRTFERYYTDPRPGEVSADQVAVIVDRTITTEDLLEIAANAGHRP
                     LAFLVLAGPTLAAHSTGVTLIRSALSGAARIGRGDVVAIPLDRRATRAKRDQVLAAYS
                     GGELLELSPRHTAEARHGLVLFFTGLSGSGKSTVARSVRDAIVEDGARSVTLLDGDLV
                     RRHLSAGLGFSAADRETNIRRIGWVGAEISRHGGVAICSPIAPFASTRDAVRDMVTEA
                     GGDFLLIHISTPLAECERRDRKGLYAKARRGEIPEFTGISSPYEDPTDADLAIDTTG"
     gene            1337..2347
                     /locus_tag="PKU75_11255"
     CDS             1337..2347
                     /locus_tag="PKU75_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81502.1"
                     /translation="MGNRWRQRGVIIPPLAELPLITVTTALETMSWAAVQWKVDSGRW
                     QRFHQGVYITHSGPVDWPTRAVAGWLSVTRPSTAEIHRLSKEPGYLLEQRLAGLGGWS
                     AAYVEGLTTDEPEVVELVVPRRRSPAVPEGVRLTRSDLPMVRANLWLPRTSIESTVIM
                     LAAREDTDSTLALVGRALQDRKTTMTRLAGELSRWTRHPAGCWLPEVLTSDNDGAEGT
                     LELRWARDVEQAHGIPPASRQARQLIGGVGCRHDLTYDEARLRIELDGFLFHDRARAR
                     TDRKKANATVVSGRSQLRYGWSEVVDEPCVTAAEVITFASSRGARWTTHPCRRPHCAV
                     VR"
     gene            complement(2407..3588)
                     /gene="galK"
                     /locus_tag="PKU75_11260"
     CDS             complement(2407..3588)
                     /gene="galK"
                     /locus_tag="PKU75_11260"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPF81503.1"
                     /translation="MQLRTHFAETFGRAPEGVWSAPGRVNLIGEHTDYNDGLALPIAL
                     PQRTYAAAARRDDDRLRVRSVQTVEVVEVALDDIGPGTPGGWAAYAAGVPWALREAGY
                     AVGGADVILDGRVPLGSGLSSSAALECAVGAALSDLYGLGLLADDANRRRLVEVCRRA
                     ENDIAGAPTGGMDQSASLLATAGHALLLDCRDGTTEQVPFPIDEAELTLLVIDTRAEH
                     ALVDGQYAARRTSCERAAAALGVPSLREIEPADLTTAATRLGAEGHVEGESSEVLARR
                     VRHVVTEIARVREAVAVMRAGDWVELGRLFTASHASLRTDFEVSCPELDLVTAAAESA
                     GALGARMTGGGFGGSAIALVPKELVERVTELLKRLAIDAGFPEPATFAVYPGPGAGRD
                     D"
     gene            3656..4681
                     /gene="trpS"
                     /locus_tag="PKU75_11265"
     CDS             3656..4681
                     /gene="trpS"
                     /locus_tag="PKU75_11265"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPF81504.1"
                     /translation="MSSDTTAVARPKRIFSGMQPTSASLHLGNYLGALVNWVALQEEY
                     DAFYGVVDLHALTVQTEPAVLRERSRRTAAQYLAGGVDPARSTLMLQSMVPEHTELSW
                     LLACHTAFGEANRMTQFKDKIAKGQSANVGLFTYPILMAADILLYDADLVPVGEDQRQ
                     HLELTRDLAERVNGRFPGTFVVPDAYIVKGSAKIMDLQDPTAKMSKSLSSDLGLIDLL
                     DDPKRITKKIKSAVTDPEREIRYDVDAKPGVSNLLVIHSTLSGTPIAELERAFEGKGY
                     GDLKGEVAEVVVAALEPFQSRMAELMADPAELDRIITAGSDKAREVASVVLGRLHDAL
                     GLLPRVR"
     gene            4743..5267
                     /locus_tag="PKU75_11270"
     CDS             4743..5267
                     /locus_tag="PKU75_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HPF81505.1"
                     /translation="MLTIGVAIDVPEPFGSVLQQARVAAGDPLAKAIPPHVTLLPPTV
                     VSGPESMEFEAHCERVAAAYRPFELELHGTGTFRPVSPVVFVALAQGAEQCSALERAV
                     RAGPVERLLDFPYHPHVTIAHHLPEEALAAAECSLADFRAAFTVSAFHLYHHGADEIW
                     RPVVAFELGARVPR"
     assembly_gap    5454..5553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5554..6135
                     /locus_tag="PKU75_11275"
     CDS             <5554..6135
                     /locus_tag="PKU75_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HPF81506.1"
                     /translation="VLSGVDLPWRAVRNGALFGGVALTLLQTFGTRLIAGTMDNKLFA
                     SIALVVGLLAFINLISRAVLLSAAWAANDVEALLASAQSQVSEGEQHKLSEGPLTREQ
                     AAAEWTDRSVGGGVPRGAGGRVAAQREAAGPVAAQRSSADRAAAGLATFDQRGADRAS
                     VASGAVLGAAGAAVVGAAVRAARGIRGRSANRR"
     gene            6226..7308
                     /locus_tag="PKU75_11280"
     CDS             6226..7308
                     /locus_tag="PKU75_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPF81507.1"
                     /translation="MSDPLSRFHAVIPAGGAGTRLWPLSRAGSPKFLHDLSGSGHTLI
                     QDTVARLRPLVGERIFIVTGVRHAEAIRAQLPDVPAAAVLVEPTPRDSMAAIGLAAAV
                     LEARDPDAILGSFAADHVVIDQSAFQECIREGVGLAADDHLVTIGITPTYAATGFGYV
                     QSGRPVTGFPTALHVRQFVEKPDARQAAAYIATGEFRWNAGMFVVRAARLLDLLGRWH
                     PQFAADLRAIAAAPERLEEMWPALPKIAIDHAVAEPAADEHAVVVVPGAFGWDDIGDF
                     SSLAALLPDPGRGTRVLGERDLVHALDATGLVVPGSQRTVTVLGLEDVIVVDTPDALL
                     VTTRERAQDVKALVEHLRDHGRTDLI"
     gene            complement(7496..7972)
                     /locus_tag="PKU75_11285"
     CDS             complement(7496..7972)
                     /locus_tag="PKU75_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPF81508.1"
                     /translation="MVTIQDAALDFLSVRRIAVTGVSHAPHGHGSNAVYQRLKERGYE
                     VFAVNPNAPEVEGDPAYPALTAIPGGVDAVVIATSPEHAMGTMKEAVDLGITKAWMHR
                     SFGTGSVSAEATSYGREHGITVIDGGCPLMFGPTADTGHKWMCRMLKLTGKVPRTV"
     gene            complement(8012..8770)
                     /locus_tag="PKU75_11290"
     CDS             complement(8012..8770)
                     /locus_tag="PKU75_11290"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HPF81509.1"
                     /translation="MTAILRKASAAVSGSVAVTGTWEGVERSAWRGLFSLGAGAAVFV
                     IAMIPLQAALFIVSPPPATAAEYFDLFQDRPLLGLLDLDLLLILDYLVMIPLYVALYA
                     VIRPVAPAWALLALILGLFALVLYVVSREATFSMWMLSNDYAAATDAGDRAAIRAAGV
                     TLLTLYNGGTFATSYLLGALSTLVFSAALLRHKIFGTLPGRLGLVTGLTMLIPPNVGQ
                     AGLVLSMLSLLPTAAWLALLSAHLFRLGRDTAAP"
     gene            complement(8767..9513)
                     /locus_tag="PKU75_11295"
     CDS             complement(8767..9513)
                     /locus_tag="PKU75_11295"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HPF81510.1"
                     /translation="MTQLESRPNQLHRTPETEASWRRLYRAGAVAGFVATALLIGDIA
                     LSGVGTVPETASAWLAQLRSNPWLGLRNLDLLNASLAVVTLPLSLAIYGAHRRSEPAL
                     TALGVLLGAVGAALFLGANVALPMLDVADRCTQDCPAAAEALLARGAHGSLGALPGFL
                     VSEAGSLLLAWTMVRGGVFGRATAWAGLLGTAGLIAYTVAATAAGGTSGPLLMLALPA
                     GVLALVWQVRTSLRLWALGRRDTLSHQGLP"
     gene            9570..10148
                     /locus_tag="PKU75_11300"
     CDS             9570..10148
                     /locus_tag="PKU75_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPF81511.1"
                     /translation="MSARVPLTKDRLFARALAIVDADGLDALTMRRLASEFGVEAASL
                     YHHVSGKDEVLDGALALMRREMTFADPLPQDWPDLMEVVFVRYLEVLMSHPHLLPLAG
                     RHLESDPAEGLPYLVAQGLPVAQAVELWQGLMAVTVGFAVFATRSFERQSAYLGGELA
                     EAMATWSSRTARHTIRHIIAPHVAGSRVDETA"
BASE COUNT         1471 a         3518 c         3611 g         1506 t
ORIGIN      
        1 atgccggtcg tgtcgatcgc caggtcggcg tcggtcgggt cctcatacgg gctgctgatg
       61 cccgtgaact ccgggatctc cccgcgccgc gccttggcat acaagccctt gcggtcccgc
      121 cgctcgcact cggcgagcgg agtggagatg tggatcagca ggaagtcgcc gcccgcctcg
      181 gtgaccatgt cgcgcaccgc gtcacgggtg ctggcgaagg gggcgatggg agagcagatg
      241 gcgactccgc cgtgccggga gatctcggcg cccacccacc cgatccggcg gatattcgtc
      301 tcccggtcgg ccgcggagaa gccgagaccg gcggacaggt ggcgccggac caggtcgccg
      361 tcgaggaggg tgacgctgcg ggcgccgtcc tcgacgatgg cgtcgcgcac gctgcgggcg
      421 acggtggact tgccggagcc ggacaggccg gtgaagaaga gcacgagccc gtgccgggcc
      481 tcggcggtat gccgcgggct cagctcgagc agttcgccgc cgctgtatgc cgcgagcacc
      541 tggtcgcgct tagcgcgcgt cgcccggcgg tcgagcggga tggcgacgac atcgccccgg
      601 ccgatgcgcg cggcacccga cagcgccgag cggatcagcg tcacgccggt ggaatgcgca
      661 gccagggtcg gccctgccag caccaggaag gccaagggcc ggtgcccggc gttcgcggcg
      721 atctcgagca ggtcctctgt cgtgatcgtg cggtcgacga tcaccgcgac ctgatccgct
      781 gacacctcgc ccggacgggg atcggtgtag tagcgctcga aggtccgcgg tgagggactc
      841 gacagccacg ccggctcggc ctcgacctcc agacacccgt catggacggt ctcgcccgtc
      901 gcggcgaggc gcgcgagcgg cactccctcc gggtcgacga tttccacagc cccggcgctc
      961 gcggcttgct cggcgattgc ggcgggcagg tgcacgctca ccggcccacc cgggctgacc
     1021 gttatcccag gaccaaagac gccgcgccgg agcaactcga ggtcgtcgag cacatccggt
     1081 ggcgggcaat actgtggtgg actcggagcc tgggtcacga ccgaccattg tgccagccga
     1141 tatgtcccac ccgtcacacc ctcgactcgc gcgcgccgtt ccaggcctgt tgctcagtcc
     1201 cgctagctgc gtgcagatgc ccgttgccgc gaaatcactg ctttagcagt gataaggcaa
     1261 cacacataac cgagcgcgaa acggcacttg tggataaccc gacgggctcg gcgccagcgc
     1321 cgggcacgat cgagacatgg gcaatcggtg gcggcagcga ggggtgatta tcccgcctct
     1381 tgccgagctg ccgctcatca cggtcacgac ggcgctcgag acgatgtcct gggccgccgt
     1441 ccagtggaag gtcgacagcg gacgctggca acgctttcac cagggggtct acatcacgca
     1501 cagcgggccg gtcgattggc ccacgcgcgc cgtggccggg tggctcagcg tgacacggcc
     1561 gtcgacggcg gagattcacc gcctctcgaa ggagcccggc tacctcttgg agcagcgtct
     1621 ggctggcttg gggggctggt ccgccgccta tgtcgagggt ctcaccaccg acgagccgga
     1681 ggtcgtggag cttgtcgtcc cacgccgccg cagccccgca gttcccgagg gggtgcgttt
     1741 gacccggtcc gatctgccca tggtgcgggc caatctgtgg cttccgcgca cgagcatcga
     1801 gtcgacggtg atcatgcttg ccgcgcgcga ggacaccgat tcgacgttgg cgctagtcgg
     1861 ccgtgccttg caggatcgca agaccacgat gacccggctg gccggcgagc tatcgcgctg
     1921 gacgcggcat ccagcaggtt gctggctccc cgaggtcctg acctccgaca acgacggagc
     1981 cgagggcacg ctggagttgc ggtgggcccg tgacgtcgaa caggcccacg gcatccctcc
     2041 tgccagtcgc caggcccgcc aactgatcgg aggtgtcggc tgccggcacg atctgacgta
     2101 cgacgaggcc cgcctgcgca tcgagctcga cggcttcctc tttcacgatc gcgcccgggc
     2161 gcgaaccgac cgcaagaagg ccaacgcaac ggtggtctcc gggcgctcgc aattgcgcta
     2221 cggctggagc gaggtcgttg acgagccctg cgtgaccgcc gcggaagtca tcacgttcgc
     2281 cagctcacgg ggcgcccgct ggaccaccca cccttgtcga cgcccccatt gcgctgtcgt
     2341 ccgctgactc ttgggtgtgg cgaaatccgc gccaaggcac agatatcgcc acacgttgtt
     2401 agcgcgttag tcgtcacggc cggcgccggg tccggggtat accgcgaagg tcgccggctc
     2461 ggggaagcca gcgtcgatgg cgaggcgttt gaggagttcg gtcacccgct cgaccagctc
     2521 ctttggcacc aacgcgatgg ccgagccgcc gaagccgccg ccggtcatcc gggcacccag
     2581 ggcaccggcg gattccgcgg ctgcggtcac caggtcgagt tctgggcagg agacctcgaa
     2641 gtcggtccgc aatgacgcat ggctcgcggt gaacagccgg ccgagctcga cccagtcgcc
     2701 cgcccgcatc acggccacgg cctcccggac tctcgcgatc tcggtgacga catgccgcac
     2761 ccgccgcgcc agcacctccg atgactcacc ctcgacgtga ccttcagccc ccagccgggt
     2821 ggccgccgtc gtcagatcgg cgggctcaat ctcacgcagg ctcgggacac ccagcgccgc
     2881 ggcggcgcgt tcgcaggagg tgcggcgggc ggcatactgc ccgtccacca acgcatgctc
     2941 ggcgcgggtg tcgatgacga gcagggtcaa ctcggcctcg tcgatgggga agggcacctg
     3001 ctcggtggtg ccgtcgcggc agtcgagcag cagcgcatgc ccggcggtcg cgagcagcga
     3061 ggccgactgg tccatgcccc cggtgggggc gcccgcgatg tcgttctcgg cgcgacggca
     3121 tacctcgacc agacgtcgac ggttggcgtc gtcggccagc aggcccaggc cgtagagatc
     3181 ggacagggcg gcgccgacag cgcactcgag cgccgccgaa ctcgacaatc ccgacccgag
     3241 cggcacccgg ccgtcgagga tgacgtcagc tccgcccacg gcatacccgg cctcgcgcag
     3301 cgcccacgga accccggcgg catacgccgc ccagccaccg ggcgtgccgg gcccgatgtc
     3361 gtccagggcg acctcgacga cttcgacggt ctggacgctg cgcacccgca accggtcgtc
     3421 atcgcggcgg gccgcggcgg catacgtccg ctgcggcaac gcgatcggca gggccagccc
     3481 gtcgttgtag tcggtgtgct caccgatcag attcacccgg cccggcgccg accagacccc
     3541 ctcgggcgcg cgtccgaagg tctcggcgaa atgcgtgcgc agctgcattc gggcgagtct
     3601 acggacgacc gataccggtt tgggcgcgtc ggtcccggct gacacaatcg cgggcatgtc
     3661 cagtgacacc accgccgtcg cgcgtcccaa gcgcatcttc tccggtatgc agccgacctc
     3721 cgcctcgctg catctcggca actacctcgg cgcgctggtc aactgggttg ccctgcagga
     3781 ggagtatgac gcgttctacg gggtcgtcga cctgcacgcc ctgaccgtcc agaccgagcc
     3841 ggccgtgctg cgggagcggt cgcggcgcac ggcggcgcag tacctcgccg gcggcgtgga
     3901 ccctgcccgc tcgacgctga tgctgcagtc gatggtgccc gagcacaccg agctgtcgtg
     3961 gctgctggcc tgccacaccg ccttcggcga ggcgaaccgg atgacccagt tcaaggacaa
     4021 gatcgccaag ggccagtcgg ccaatgtcgg gctgttcacc tacccgatcc tcatggcggc
     4081 cgacatcctg ctctacgacg ccgacctggt gccggtgggc gaggaccagc gccagcacct
     4141 ggagctcacg cgcgatctcg ccgagcgggt caacgggcgc ttcccgggca ccttcgtggt
     4201 gcccgacgcc tatatcgtca agggctcggc caagatcatg gacctgcagg accctacggc
     4261 gaagatgtcg aagtcgctct cgtcggacct aggtctgatc gacctgctcg atgaccccaa
     4321 gcggatcacc aagaagatca agtccgcggt caccgacccc gagcgggaga tccggtatga
     4381 cgtggacgcc aagcccggcg tgtcgaacct gctggtgatc cactcgacgc tgtcggggac
     4441 gcccatcgcg gaactggagc gggcgttcga gggcaagggc tatggggacc tgaagggcga
     4501 ggtggccgag gtcgtcgtcg ccgctctcga gcccttccag tcgcggatgg ccgaactgat
     4561 ggccgacccg gccgagctgg accggatcat cacggccggg tcggacaagg cccgcgaggt
     4621 ggcgtcggtg gtgctcggcc gcctgcacga cgccctcggg ctgctgccgc gggtccgatg
     4681 aaacccgata cttcgggcac gcgttttcgc gttgaccccg cgcaccggta gcctccgttc
     4741 tcgtgctcac gatcggggtc gccatcgatg tgcccgaacc cttcgggagc gtgctgcagc
     4801 aggcgcgggt cgctgccggc gacccgctgg ccaaggccat cccgccgcac gtgaccctgc
     4861 tgccgccgac cgtcgtcagc ggacccgagt cgatggaatt cgaggcgcac tgcgagcggg
     4921 tggcggcggc ataccgtccc ttcgaactgg aactgcacgg caccggcacc ttccggccgg
     4981 tctcaccggt cgtcttcgtc gccctcgcgc aaggcgcgga gcagtgctcg gcgctggagc
     5041 gcgccgtgcg agcggggccg gtcgagcggc tgctggattt cccctatcac ccgcacgtga
     5101 ccattgccca ccacctgccg gaggaggcgc tcgccgcggc cgagtgctcg ctggcagatt
     5161 ttcgggccgc gttcacggtc tcggccttcc atctctatca ccatggagcc gatgagatct
     5221 ggcggcccgt ggtcgccttc gaactcgggg cccgcgtgcc ccggtgacgg acacaggcgt
     5281 gccggtatgc agtcgctgaa ggatctttgg aagcgcttca gcgtctcgtc ggtctggcgc
     5341 gcgtggcagc ggtattccac cgcccgcggc gggctgctcg ccggcggcgt gacctatttc
     5401 gccttcttct ctctcttccc ggccatcgcg ctcggcttca cgatcttcgg gctnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgctgt cgggcgtgga cctgccgtgg
     5581 cgggcggtgc gcaatggcgc gctcttcggg ggcgtggcgc tgaccttgct gcagacgttc
     5641 ggcacgcggc tgatcgccgg gacgatggac aacaaactgt tcgcctcgat cgccctggtc
     5701 gtcgggttgc tcgcgttcat caacctgatc tcccgggcgg tgctgctgtc ggcggcgtgg
     5761 gcggccaatg acgtcgaggc gctcctggcc tcggcccagt cgcaggtcag cgagggggag
     5821 cagcacaagc tctccgaggg gccgctgacc cgggaacagg ccgctgccga atggacggac
     5881 cggtctgtcg gcggcggggt gccgcgcggg gctggcggcc gggtggcagc gcagcgggag
     5941 gcggcgggcc cggtggcagc gcagcggtcc tcggcggacc gggctgcagc ggggctggcg
     6001 accttcgacc agcgcggtgc cgaccgcgct tcggtcgcct ccggcgccgt cctcggcgcg
     6061 gcgggcgccg ccgtcgtggg cgcggccgtg cgcgcggcgc gggggatccg aggaaggtct
     6121 gctaacagac gctagtcaaa tcagcagacc tccctaggtg tgcagattcg accagcctct
     6181 gctgagtggc ggtcccgacc gcccgcggcg gctagcctgc ctgccgtgag cgatccgctg
     6241 tctcgattcc acgccgtcat acccgcgggc ggggccggca cccggttgtg gccgctctcg
     6301 cgcgccgggt cgccgaagtt cctccacgac ctgtccgggt ccgggcacac gctcatccag
     6361 gacaccgtcg cccggctgcg gcccctcgtc ggcgagcgga tctttatcgt caccggcgtc
     6421 cggcacgcgg aggcgatccg cgcgcaactg cccgacgtgc ccgccgcggc cgtgctggtc
     6481 gagcccaccc cgcgcgactc gatggcggcg atcgggctgg ccgcggccgt gttggaggcc
     6541 cgcgacccgg acgcgatcct cgggtccttc gccgccgacc acgtggtcat cgaccagtcc
     6601 gccttccagg agtgcatccg ggagggggtg gggctcgccg cagacgatca cctggtgacg
     6661 atcggcatca ccccgacgta tgccgcgacg ggcttcgggt atgtgcagtc gggtcgcccc
     6721 gtgaccgggt tccccaccgc gttgcatgtg cgccagttcg tcgagaagcc ggatgcgcgg
     6781 caggccgcgg cgtacatcgc gaccggcgag ttccggtgga acgccgggat gttcgtcgtg
     6841 cgggccgccc ggctgctgga cctgctcgga cgatggcacc cgcagttcgc cgccgacctg
     6901 cgggccatcg ccgccgcgcc ggagcgcctc gaggagatgt ggccggcgct gccgaagatc
     6961 gccatcgacc acgctgtcgc cgaaccggcc gcggacgagc acgccgtcgt cgtcgtgccc
     7021 ggtgccttcg gttgggacga catcggcgac ttctcttctc tcgccgccct cctgcccgac
     7081 ccggggcgcg ggacgcgggt cttgggggaa cgcgacctgg tgcatgccct ggacgcgacg
     7141 ggcctggtgg tccccgggtc gcagcgcacc gtgaccgtgc tcggcctcga ggacgtcatc
     7201 gtcgtcgaca ccccggacgc ccttctggtc accacccgcg agcgcgccca ggacgtcaag
     7261 gcgctcgtcg agcacctgcg cgaccacggc cgcaccgacc tgatctgacc ccggcccggc
     7321 cgcggccgca ggggcccggg ctcgggaggt ggcgtgtggc gccgctgcac ggggcgcttc
     7381 tagcgggcgg ccggatcgcc ggtgcgggtg ccgaacggga tcgcaccaag aagacattgg
     7441 atgtcttgcg gcgtcggggc ggcgcggtcc atcgtgtagc cgcgtgcagc gacggtcaca
     7501 cggtgcgggg gaccttgccg gtcagcttca acatccggca catccacttg tgcccggtgt
     7561 ccgcggtcgg gccgaacatc agcgggcagc cgccgtcgat gaccgtgatg ccgtgttcgc
     7621 ggccatagct cgtggcctcg gccgagacgc tgcccgtccc gaaggagcgg tgcatccagg
     7681 ccttcgtgat cccgaggtcc acggcctcct tcatggtgcc catcgcgtgc tccgggctgg
     7741 tggcgatcac gaccgcgtcg actccgccag ggatggcggt gagcgcgggg taggcgggat
     7801 cgccctccac ctcgggcgca ttggggttga ccgcgaacac ctcgtagccg cgctccttca
     7861 gccgctggta gaccgcattg ctgccgtggc cgtgcggggc gtgcgagacc ccggtcaccg
     7921 cgatccgccg cacggacagg aaatccaggg cggcgtcctg aattgtgacc atcgtgtcct
     7981 ccgtcgttcg ttcgttgtcg ggtatggcgc gtcagggcgc ggctgtgtcg cggccgagcc
     8041 ggaacaagtg cgcgctcagc agggcgagcc aggcggccgt ggggagcagg gagagcatcg
     8101 agaggacgag gccggcctgg ccgacgttgg gcggaatgag catggtgagg ccggtcacga
     8161 gccccagccg gccggggagg gtgccgaaga tcttgtggcg aagcagggcc gccgagaaca
     8221 ccagggtgct gagcgctccg agcagatagc tcgtggcgaa ggttccaccg ttgtagaggg
     8281 tcaggagggt caccccggcg gcgcggatgg cggcccggtc gcctgcatcg gtcgccgcgg
     8341 catagtcgtt gctcagcatc cacatcgaga acgtggcttc ccgggagacg acatacagga
     8401 ccagcgcgaa gagcccgagg atcagcgcga gcagggccca ggcgggcgcg acgggccgga
     8461 tcacggcata cagcgccaca tagagcggga tcatcacgag gtagtcgagg atcagcagca
     8521 ggtcgaggtc cagcagcccg agcagcggcc ggtcctggaa gaggtcgaag tactcggcgg
     8581 cggtcgccgg cggggggctg acgatgaaca acgccgcctg cagcgggatc atcgcgatca
     8641 cgaagaccgc agcgcccgcg ccgagactga agagcccccg ccacgcagag cgctcgacgc
     8701 cctcccaggt cccggtcacc gcgacggacc cgctcacggc ggcactggcc ttgcggagga
     8761 tggcggtcat ggcaggccct ggtgtgagag cgtgtcccgg cgtccgagcg cccacagccg
     8821 caggctggtg cgcacctgcc agacgagggc gaggactccc gcggggaggg cgagcatcag
     8881 caacggtccg ctcgtgccgc ccgccgcggt ggcggccacg gtgtacgcga tgagcccggc
     8941 cgtgcccagc aggcccgccc aggccgtggc cctgccgaac accccgccac gcaccatcgt
     9001 ccatgcgagg aggaggctgc cggcctcgct gacgaggaat cccggcagcg cgcccaggga
     9061 gccgtgggct ccgcgggcga ggagcgcctc ggccgcggcg gggcagtcct gggtgcaacg
     9121 gtcggccacg tcgagcatgg gcagcgcgac attcgcgccg aggaagagag ccgcgccgac
     9181 ggctcccagg agcacgccga gcgcggtcag ggcgggttcg ctcctgcggt gggcgccgta
     9241 gatcgcgagg gacagcggca gggtcaccac ggccaggctc gcgttgagca ggtcgagatt
     9301 gcgtagcccg agccacggat tggagcgcag ctgggcgagc catgcgctcg ccgtctcggg
     9361 cacggtgccg acaccggaga gggcgatatc gccgatcaac agggcagtgg cgacgaaccc
     9421 ggcgacggcg ccggcccggt agaggcggcg ccaggaggct tcggtctccg gcgtgcggtg
     9481 gagttggttc ggtctgctct cgagttgcgt catgaccacg tcccctaaca ctgttaggat
     9541 cgcacctaac gctgttagag tcaaggggta tgagcgcgcg agttcccctg accaaggacc
     9601 ggctcttcgc ccgggcgttg gcgatcgtgg acgcggacgg gctggacgcg ctgacgatgc
     9661 gccgcctggc ctccgagttc ggcgtggagg ccgcctcgct ctatcaccat gtcagcggga
     9721 aggacgaggt gctcgatggc gccctggcgc tgatgcggcg ggagatgacc ttcgccgacc
     9781 ccctgccgca ggactggccc gatctcatgg aggtcgtttt cgtgcgttat ctcgaggtgc
     9841 tcatgtccca tccgcacctg ctgccgctgg ccggccggca cctggagagc gatcccgccg
     9901 aggggctgcc ctatctggtc gcgcagggac tgccggtggc ccaggccgtg gaactctggc
     9961 aagggctgat ggcggtgacc gtcggcttcg cggtcttcgc cacccgctcc ttcgagcggc
    10021 agtccgccta cctcggaggg gaactcgccg aggcgatggc cacctggtcc agccggaccg
    10081 cgcggcatac catccgccac atcatcgccc cccacgtggc gggcagccgg gtcgacgaga
    10141 ctgcctgacc tcgaccgggg agccctcgtc acgggtccgc ggtggctgat gggtcacatt
    10201 caccac
//
LOCUS       DAOPJG010000123        10194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6911_length_10194_cov_7.024460, whole genome shotgun sequence.
ACCESSION   DAOPJG010000123 DAOPJG010000000
VERSION     DAOPJG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6911_length_10194_cov_7.024460"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..841
                     /locus_tag="PKU75_11305"
     CDS             8..841
                     /locus_tag="PKU75_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPF81512.1"
                     /translation="MRTLADVVAVIEELYPPEAAQSWDQVGLVTGDPAQQVQRLHLAL
                     DPTLAVIEEARALGADLLFTHHPLLMRGVHSVATTTAKGASITNLVVGDVALYVAHTN
                     ADVASRGVAEALAAACGLDAIEPLVIEEGRPMGRVGELSERISLREFASRVSAALPPA
                     PVGLRVAGDPEAQVCRVAVSGGAGDGFFGAARAVRADVYVTADLRHHPALEAREEGRG
                     GPPFLIDAGHWASEASWLPWAADDLRERLGEDVEITVSELCTDPWTFTVVATQGAGPG
                     R"
     gene            891..1631
                     /locus_tag="PKU75_11310"
     CDS             891..1631
                     /locus_tag="PKU75_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPF81513.1"
                     /translation="MKADPRDQQRLLDLQQIDTTAAQLERQRSHLPVLAKLDALAQAR
                     MHAQDAVVTAETEQGDLERELAKAEADVQLVRDRAARDQAHLDSGKGTAKELTSLQHE
                     LASLARRQADLEEIEIEVMERLETAEAALTQARSTLAAVDADVADTIAERDEQTADLT
                     GRLAEATAPRDTVVGELPADLVALYEKLRASHGGTGAAMLHARRCLGCQLELNNADIA
                     RIRAAAPDEVLRCEECSRILVRTAESGL"
     gene            complement(1711..7572)
                     /gene="pulA"
                     /locus_tag="PKU75_11315"
     CDS             complement(1711..7572)
                     /gene="pulA"
                     /locus_tag="PKU75_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA];
                     GO_process: GO:0005983 - starch catabolic process
                     [Evidence IEA];
                     GO_process: GO:0051678 - pullulan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-type alpha-1,6-glucosidase"
                     /protein_id="HPF81514.1"
                     /translation="MHPTRSIPSAIAISALIAAGLATAVAAPVAAAERTATLVGNLQD
                     EISSCADWTPDCAPTDLTQVGTTPIYERIFEVPAGSYEFKVAINHDWEESYGGPSGNI
                     PLVLAGPARLKFSYNDENHKIGVTPMDMAGPVTPADKALAGNSLRNGLTKERFYFVMA
                     DRFANGSTANDKGGLTGDRLATGFDPTDKGFYHGGDLKGVINKLDYIKGLGTTAIWLT
                     PSFKNRPVQGSGADASAGYHGYWVTDFTQIDPHLGTNAEMKALINQAHAKGMKVFFDI
                     ITNHTADVISYAENQYGYVSKSEEPYKDASGAEFDDRDYAGGNTFPPLDPQVSFPYTP
                     VFRTPADATVKVPSWLNDRTLYHNRGDSTFAGESSEYGDFVGLDDLFTEHPDVVRGMG
                     DVYKTWVDFGIDGFRIDTVKHVNMQFWQRFAPDVLARAKAIGNNDFFMFGEVFSGDPA
                     VMSQYTTTGKLPATLDFGFQEAAIDFAGGAATSRLRNLFYGDDYYTDLDSNVYESPTF
                     LGNHDRGRASWMISTKGFTGAELLKRVKLANSLMFLTRGQPVVYYGDEQGFIGSGGDK
                     DARQDMFATKVAQYAAEPNLTGPSGSRNRYSTTAPLYVQTKQLAALRQAHPALANGAQ
                     THRYASGSAGIYALSRVDRASNIEYLVVANNATTAKTATFDTFTKNVGYTPIYGGGTA
                     IRSAAGGRVNVTVPALGVRVFKARAKIPARAYAPPVYLTSPSAGDVVSGRAEIAAATT
                     QDVFAQTTFAYRPVGTTQWQKIGTDDNAPYSVYHDVSAMTAGTMLEYRTIVKDSSGNF
                     SAASSYAVVGSPNARTGKSGAAAGNSVARAKGGMPAQQISRSSTAGRTQGKGLVAKAI
                     GPVTQPDFVSVPGNLNTEMGCAGDWQPECDQAQMTLDPNDQIWKLTVDLPAGDYEYKA
                     AINKLWEESYGQGGTPGGSNILLNFSGGSITFYYDHGTHWITSSAQGPVITAPGSMQS
                     ELGCPEDWQPQCMNPWLQDPDEDGTYTWSSDQIPVGSYEFKIAHNLSWDENYGEGGTP
                     AGNNIPFSVPRAGTVVTITYVLATHQITVTTSQSGAAPDLKAAKAFWIEPGLLAWPAD
                     AVPEGANLKLLKWRLHWASNAGMTIDDEDLPGSTSAGLAYDPNGLPASVTASRPELKG
                     YIALRAGRTVKDLAPTILRGQVAVALYSNSGQVLDATGVQTAYVLDSLYAASAKTKKY
                     GAVFTGANPTYRVWAPTAKKVTLLTWAPSAPANAPLSAATRTGMTRAGDGSWSVTKGP
                     KNARYLYEVVVYSRSTAKIETLAVTDPYSVALTLNSTKSVAVNLKDGAYKPAVFNSPQ
                     PKLPQNVDHSIYELHVRDFSITDTSVAPELRGSYLAFNTNGRGMQHLKKLRAAGLNTI
                     HLLPTFDIASIEEDPAKQSTPQCDLESYPPDSEQQQECIEAIRSNDAFNWGYDPFHWL
                     APEGSYASTAATADGGKRVAEFRTMVGGLHKAGLRVVLDKVFNHTPAAGLAPTSVLDK
                     VVPDYYQRLDKTGNVYTSTCCQNIATEHQMAQKIMVDGVVMWARDYKIDGFRFDLMGH
                     HSKANMLAIRAALNQLTVANSGVDGKKVYLYGEGWNFGEVADNALFYQATQGQLGGTH
                     IGTFSDRLRDAVRGGGPFDDDPRKQGFGSGEFTDPNGAPINSGAQAGLKHDTDLVQLG
                     LAGNLKAFSFRLNSSGAVARGDQVDYNGSPAGYATQPDEVITYVDAHDNETLFDSLTF
                     KLPVATTMSDRIRMNTLSLATTALAQTPSFWHAGADLLRSKSLDRNSYDSGDWFNRLD
                     WTGADNGFGHGLPLEGDNGAKWPYMKPLLANSALKPNSAQVMTATAQAQDLLKLRYST
                     RLFRLGTAGAIKAKLTFPASGTANAIPGVIAMRIDDTVGADIDPNLKGLVVVFNATPE
                     AVTQTVPGMAGKALSLSPIQANGSDPVVKNAKWNTAAGSATVPARTVAVFLQK"
     gene            7729..8925
                     /gene="metZ"
                     /locus_tag="PKU75_11320"
     CDS             7729..8925
                     /gene="metZ"
                     /locus_tag="PKU75_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HPF81515.1"
                     /translation="MSDPNERGDLHRETLAVRAGLHRSAFDETAEAMYLTSGYVYDTA
                     GEAEATFLEEVDRYSYSRYANPTVTMFEERLAALEGAQAAYATPTGMSAVFAALGGLC
                     AAGDRIVASKALFGTCYTVCAEILPRWGVEAVFVDGTDLDAWREALSVPTKAVFFETP
                     SNPTMTLVDIAAVSELAHAAGATVVVDNVFGTPLFSSPLQHGADLVVYSATKHIDGQG
                     RVMGGAVLGSAQLIKDVVRPMIRVLGLTMSAFNAWVLVKSLETMDLRLTKQAQGALAV
                     AEWLEANPLVSQVFYPGLASHPQAELIARQMTGHGTVVTFTLDTDRAGAFRALDALEI
                     VDISNNLGDTKSLATHPATTTHSKIPEAERLALGITDGTIRLSVGLEHPDDLIADLER
                     AFAAVR"
     gene            9171..10145
                     /locus_tag="PKU75_11325"
     CDS             9171..10145
                     /locus_tag="PKU75_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HPF81516.1"
                     /translation="MDRRLDAVTAATGGVFTTKDAAAVGIRHEEIAHMVRRKEVIRVR
                     RGAFVSGQVYRDADVDERYRLQVMAALRLRPTDAAAAHAALALRRLPTWGADLRRIDL
                     AGAVKHHESGAGLRVRPRGDLEVVTIGGRASVRIADALVQHAAQSGIVAGVVAMDAAV
                     HAKLVTVASIAEAIERMPRSGRRRARAAATLIDPACESVGESRTRLLLLDFGLAFESQ
                     ARLYERRTLIGRVDFLVEERVVVEFDGLVKYSGLDGKEVLAAEKARESRLVALGYEVV
                     RLTWRELDDPVGVIARIRDALARALTRPAPPPRPPEPRTASEAANSLV"
BASE COUNT         1492 a         3235 c         3710 g         1757 t
ORIGIN      
        1 ggcccacgtg cgcaccctcg ccgatgtcgt tgccgtgatc gaggagctgt atccgcccga
       61 ggcggcgcag tcgtgggacc aggtgggact ggtcaccggc gacccggccc agcaggtcca
      121 gcggcttcac ctcgcgttgg acccgacgct ggcggtgatc gaggaggccc gggcgctcgg
      181 cgccgatctg ctcttcaccc accatccgct gctgatgcgc ggcgtgcatt ccgtcgcgac
      241 gacgacggcg aagggggcga gcatcaccaa cctcgtcgtc ggggacgtcg cgctgtatgt
      301 cgcccacacc aatgccgatg tcgcctcacg cggtgtggcg gaggcgctgg cggccgcgtg
      361 tggcctcgac gcgatcgagc cgctggtgat cgaggagggg cggccgatgg gccgcgtggg
      421 agagctgtcg gagcggattt cgttgcggga gttcgcctct cgtgtttccg cggcgcttcc
      481 gccggcgccg gtggggctgc gcgtcgcagg tgacccggag gcacaggtat gccgggtggc
      541 cgtctcgggt ggggcgggtg acggcttctt cggggccgcg cgtgcggtgc gggcagacgt
      601 ctatgtgacg gccgacctgc ggcatcatcc ggcgctggag gcgcgcgagg agggccgggg
      661 cggcccgccg ttcctgatcg acgcgggaca ctgggcgagc gaggcctcct ggctgccgtg
      721 ggccgccgac gacctgcgtg agcgcctggg cgaggacgtc gagatcacgg tgagcgaatt
      781 gtgcaccgat ccgtggacct tcaccgtcgt tgccacgcag ggtgctgggc ccgggcgttg
      841 acgactcgtg cggataggtt ggtctctacc aactcaagaa ggatggccga gtgaaagccg
      901 acccgcgaga ccaacagcgg ctgctggacc tgcagcagat cgacaccacc gccgcgcagc
      961 tggagcgcca gcgctcgcac ctgcccgtgc tcgccaagct ggacgcactg gcccaggcgc
     1021 ggatgcacgc gcaggacgct gtggtgaccg ccgagaccga gcagggcgac ctggagcgcg
     1081 agctggccaa ggcggaggcg gacgtgcagt tggtccgcga ccgcgcggcg cgcgaccagg
     1141 cccatctgga ttcgggcaag ggcacggcga aggaactcac gtcgctgcag cacgagctgg
     1201 catcgcttgc gcgtcgacag gccgatctcg aggagatcga gatcgaggtc atggagcggc
     1261 tcgagaccgc ggaagcagcc ctgacgcagg cgcgctcgac gctggccgcc gtggacgcgg
     1321 acgtcgccga caccatcgcc gagcgcgacg agcagacggc cgatctgacg ggccggctcg
     1381 cggaggcgac tgcgccccgt gacaccgtcg tgggggagtt gcccgccgac ctcgtggcgc
     1441 tctatgagaa gttgcgcgcc agccacggcg ggaccggcgc ggccatgctg cacgcgcgtc
     1501 gctgcctggg gtgccagctc gaactcaaca acgccgacat cgcgcggatc cgcgcggccg
     1561 cgcccgatga ggtgctgcgc tgcgaggagt gcagccggat cctcgtgcgc accgccgaat
     1621 ccggtctctg acctcggtat gtcgcggggc tgagaaaccg gcccgacccc cgaatgagaa
     1681 gggggccggg ccggtttcga cgcggtgatc ttacttctgg aggaagaccg ccacggtgcg
     1741 ggcgggcacg gtcgccgagc cggcagccgt gttccacttc gcgttcttga cgacggggtc
     1801 ggagccgttc gcctggatcg gcgagaggct gagcgccttg ccggccatgc ccgggacggt
     1861 ctgggtgacc gcctcggggg tggcattgaa gacgacgacg agacccttga ggttcgggtc
     1921 gatgtcggcg ccgaccgtgt cgtcgatgcg catcgcgata acgccgggga tggcgttcgc
     1981 cgtgccgctg gccgggaagg tcagcttggc cttgatcgcg cccgccgtcc cgagccggaa
     2041 caggcgagtg gagtagcgca atttgagcag gtcctgcgcc tgcgcggtgg ctgtcatgac
     2101 ctgcgcggag ttcggcttca gggcgctgtt ggcgagcagc ggcttcatgt acggccactt
     2161 cgcgccgttg tcgccctcga gcggcaagcc gtgaccgaag ccgttgtcgg cgccggtcca
     2221 gtcgagccgg ttgaaccagt cgccactgtc atacgagttg cgatcgagcg acttgctgcg
     2281 caacaggtcg gcacccgcat gccagaacga cggcgtctgg gccagggccg tcgtggctag
     2341 cgagagggtg ttcatccgga tccggtcgga catcgtcgtc gcgaccggca gcttgaaggt
     2401 caagctgtcg aacagcgtct cgttgtcgtg ggcgtccaca taggtgatga cctcatcggg
     2461 ctgcgtggca tagccggcgg gggagccgtt gtagtcgacc tggtcgccgc gcgccacggc
     2521 gccggaggag ttgagccgga agctgaacgc cttcaagttg ccggccaggc cgagctggac
     2581 caggtcggtg tcgtgcttca ggcccgcctg ggcgccggag ttgatcggcg cgccattggg
     2641 gtcggtgaac tcaccgcttc cgaagccctg cttgcgcgga tcatcatcga acggtccgcc
     2701 accgcgcacg gcatcgcgca accggtcgct gaaggtgccg atgtgtgtgc cgccgagctg
     2761 gccctgggtt gcctggtaga acagcgcgtt gtcggcgacc tcgccgaagt tccaaccctc
     2821 gccgtagagg tagaccttct tgccgtcgac accgctgttg gcgaccgtca gttggttgag
     2881 cgcggcccgg atcgccagca tgttcgcctt cgagtgatgg cccatcaggt cgaaccggaa
     2941 gccgtcgatc ttgtagtcgc gcgcccacat cacgacgccg tcgaccatga tcttctgcgc
     3001 catctggtgc tcggtcgcga tgttctggca acaggtcgag gtgtagacgt taccggtctt
     3061 gtccaggcgc tggtagtaat ccggcacgac cttgtccagc acactggtcg gggcgagccc
     3121 ggccgcgggg gtgtggttga acaccttgtc gaggaccacc cgcagtcccg ccttgtgcaa
     3181 gccgccgacc atcgtgcgga actcggcgac tcgcttgccg ccgtcggcgg tggctgcggt
     3241 cgaggcgtag ctgccttccg gcgccagcca gtggaacggg tcgtagcccc agttgaacgc
     3301 atcgttgctg cggatggcct cgatgcattc ctgctgctgc tcactgtcgg gcgggtagct
     3361 ctccaggtcg cactgcgggg tcgactgctt ggccgggtcc tcctcgatcg aggcgatgtc
     3421 gaaggtcggg agcaggtgaa tggtgttcag cccggccgcc cgcagctttt tcaggtgctg
     3481 catgccgcgg ccgttggtgt tgaacgcgag gtaggagccg cgcaactcgg gtgccacgct
     3541 ggtgtcggtg atcgagaaat cccgcacgtg caactcgtag atcgagtggt cgacgttctg
     3601 cgggagcttc ggctgcgggc tgttgaacac ggccggtttg taggcgccgt ccttgaggtt
     3661 gacggccacc gacttggtcg agttcagcgt gagcgcgacc gagtacggat cggtgaccgc
     3721 gagggtctcg atcttggcgg tcgagcgcga atagacgacc acctcgtaga ggtagcgcgc
     3781 gttcttcggc cccttggtca ccgaccacga cccgtcgccg gcacgggtca tcccggtgcg
     3841 ggtcgcggcg ctgagcgggg cgttcgcggg cgccgacggc gcccacgtca acagcgtgac
     3901 cttcttcgcg gtcggggccc agacccggta ggtggggttc gcgccggtga agaccgcccc
     3961 atacttcttg gtcttggcgc tcgcggcata gagcgagtcc aggacgtatg ccgtctgcac
     4021 gccggtggcg tccaggacct ggccgctgtt gctgtagagg gcaacggcca cctgtccccg
     4081 caggatggtg ggcgccaggt ccttgacggt ccggcccgct cgcagggcga tgtagccctt
     4141 gagctccggc cggctcgcag tgacggaggc gggcagacca ttggggtcat acgccaggcc
     4201 ggccgacgtc gaaccgggta ggtcctcgtc gtcgatcgtc ataccggcgt tcgatgccca
     4261 gtgcaggcgc cacttcaaca gtttcaggtt ggcgccctcg ggcacggcgt ccgccggcca
     4321 ggcgagcagt ccgggctcga tccagaaggc cttggcggcc ttgaggtcgg gggccgcgcc
     4381 gctctgggag gtggtcacgg tgatctggtg ggtcgccagc acataggtga tggttaccac
     4441 ggttccggcc cgcgggacgg agaacgggat gttgttcccg gccggtgtgc cgccttcgcc
     4501 gtagttctcg tcccagctga gattgtgggc gatcttgaac tcgtagctgc ccaccgggat
     4561 ctggtccgag ctccacgtgt aggtgccgtc ctcgtcgggg tcctgcagcc acgggttcat
     4621 gcattgcggc tgccagtcct ctgggcagcc gagctcggac tgcatcgacc ccggcgcggt
     4681 gatgaccggg ccttgcgcgc tgctggtgat ccagtgcgtg ccgtggtcgt agtagaaggt
     4741 gatcgacccc ccggagaagt tgagcaggat atttgagcct cccggcgtcc cgccttggcc
     4801 gtagctctcc tcccacaact tgttgatggc ggccttgtac tcgtagtcgc ccgccgggag
     4861 gtcgacggtg agcttccaga tctggtcgtt ggggtcgagt gtcatctgcg cttggtcgca
     4921 ctccggctgc caatcgccgg cgcagcccat ctcggtgttg agattgcccg gcacggacac
     4981 gaagtcgggc tgggtgaccg gcccgatcgc cttggcgacc agacccttgc cctgcgtccg
     5041 cccagccgtg ctgctacggc tgatctgctg cgccggcatg ccgcccttgg cgcgcgccac
     5101 gctgttgccg gcggcggccc cggacttccc ggtccgggcg ttcgggctgc cgacgacggc
     5161 atacgacgag gcggcgctga agttgccgct cgagtccttg acgatcgtgc ggtactccag
     5221 catggtgccg gcagtcatcg cggatacgtc gtggtatacg gagtagggag cgttgtcgtc
     5281 ggtgccgatc ttctgccact gcgtggtgcc caccggccgg taggcgaagg tggtctgcgc
     5341 gaagacatcc tgggtcgtcg ccgccgcgat ctccgcgcgg cccgagacga cgtcaccggc
     5401 ggagggcgag gtcaggtaga ccggcggggc gtatgcgcgc gccgggatct tggcgcgcgc
     5461 cttgaagacg cggacaccga gggcgggaac tgtgacgttg acccggccac cggcagcgga
     5521 acggatcgct gtgccaccgc cgtagatcgg ggtgtagccg acgttcttgg tgaaggtgtc
     5581 gaaggtcgct gtcttcgccg tggtcgcatt gttggcgacg acgaggtact cgatgttgct
     5641 cgcgcggtcg acgcgggaga gggcgtagat cccggcgctg cccgaggcgt agcggtgcgt
     5701 ctgagcgccg ttggccaggg ccggatgggc ctgccggagc gcggccaact gcttggtctg
     5761 cacatagagc ggggcggtgg tggagtaccg gttgcgggag ccggacgggc cggtcaggtt
     5821 gggctccgcg gcatactgcg cgaccttggt ggcgaacatg tcctggcggg cgtccttgtc
     5881 gccgcccgac ccgatgaagc cctgctcgtc gccgtagtag acgaccggct gtccgcgggt
     5941 gaggaacatc agcgagttgg ccagcttgac ccgcttgagc aactcggcgc cggtgaagcc
     6001 cttcgtggag atcatccacg aggcgcggcc gcggtcgtga ttgccgagga aggtcgggga
     6061 ctcgtagacg ttggagtcga gatcggtgta gtagtcgtcg ccgtagaaga ggttccgcag
     6121 ccgggacgtc gccgcgccgc cggcgaagtc gatcgcggcc tcctggaacc cgaagtcgag
     6181 ggtggcggga agcttgccgg tggtcgtgta ctggctcatg actgccgggt cgccggagaa
     6241 gacctcgccg aacatgaaga agtcgttgtt gccgatggcc ttggccctgg ccagcacgtc
     6301 cggggcgaag cgctgccaga actgcatgtt gacgtgcttg acggtgtcga tgcggaatcc
     6361 gtcgatgccg aagtcgaccc aggtcttgta gacatcgccc atcccgcgca ccacgtcggg
     6421 gtgctcggtg aacaggtcgt cgaggccgac gaagtcgccg tactcactcg actcgccggc
     6481 gaaggtcgag tcgccacggt tgtgatagag ggtgcggtcg ttcagccagg acggcacctt
     6541 gaccgtggcg tccgccggag tgcggaagac gggggtgtag gggaaggaga cctgcgggtc
     6601 cagcggcggg aaggtgttgc cgcccgcgta gtcgcggtcg tcgaactcgg ctccgctggc
     6661 gtccttgtag ggctcttcgc tcttgctgac gtagccgtac tggttctccg cgtaggagat
     6721 gacgtccgcg gtgtgattgg tgatgatgtc gaagaagacc ttcatgccct tggcgtgggc
     6781 ctggttgatg agagccttca tctcggcatt ggtgcccagg tgcgggtcga tctgggtgaa
     6841 gtcggtgacc cagtagccgt ggtagcccgc gctggcgtcg gcgccgctgc cctggacggg
     6901 gcggttcttg aagctggggg tcagccagat cgccgtggtg ccgaggccct tgatgtagtc
     6961 gagtttgttg atgacgccct tgaggtcgcc gccgtggtag aagcccttgt cggtcgggtc
     7021 gaacccggtc gcgaggcgat cgccggtcag gccgcccttg tcgttggccg tgctgccgtt
     7081 ggcgaaacgg tcggccatga cgaagtagaa gcgttccttg gtcaggccgt tgcgcagcga
     7141 gttgccggcc agggccttgt ccgccggggt caccgggccg gccatatcca tcggcgtgac
     7201 gccgatcttg tgattctcgt cgttgtagga gaacttgagc cgggccggcc cggcgaggac
     7261 gagcgggata ttgccgctcg gaccgccgta gctctcctcc cagtcgtggt tgatggcgac
     7321 cttgaactcg tagctgccgg ccgggacctc gaagatccgc tcgtagatcg gcgtcgtccc
     7381 cacttgggtc aggtcggtgg gtgcgcagtc cggggtccaa tcggcgcacg atgagatctc
     7441 gtcctgcaga ttgccgacca gggtggccgt gcgttcggcg gccgcgaccg gggcggccac
     7501 agccgtggcg aggccggcgg cgatcagggc gctgatggcg atggctgagg ggattgacct
     7561 cgtgggatgc acgtccggct cctttggcgg gggatctacc gcccgccacc atagggccgc
     7621 cacaccgaca ggcaccagat ccattgggat tgaaagatct tgcaacacgg atggtccagc
     7681 catggacgca ccagatcggg tgtccaaagg ggtcgttagt ctcgggccat gagtgatccg
     7741 aacgagcgtg gcgacctcca tcgcgagacc ctggccgtcc gggccggact gcatcgcagc
     7801 gcgttcgacg agaccgccga agcgatgtac ctcacctccg gctacgtcta cgacaccgcg
     7861 ggggaggccg aggcgacctt cctcgaggag gtcgaccgct actcatacag ccggtatgcc
     7921 aacccgacgg tgacgatgtt cgaggagcgg ctcgcggcgc tcgaaggtgc gcaggcggca
     7981 tacgcaaccc cgaccgggat gtcggcggtc ttcgccgcgc tcggcggcct gtgcgcggcg
     8041 ggtgaccgga tcgttgccag caaggcgctg ttcggcacct gctacacggt gtgcgcggag
     8101 atcctgccgc ggtggggggt cgaggcggtc ttcgtcgacg gcaccgatct cgacgcctgg
     8161 cgggaagcgt tgtccgtgcc caccaaggcg gtcttcttcg agactccgag caacccgacg
     8221 atgaccctcg tcgacattgc cgcggtgtcc gagctggcgc acgcggccgg ggcgacagtg
     8281 gtggtggaca acgtcttcgg gactccgctg ttctcctcgc cgctgcagca tggcgcggat
     8341 ctcgtcgtgt actcggcgac caaacacatc gacggccagg ggcgggtgat gggtggcgcg
     8401 gtgctcggca gcgcgcagct gatcaaggac gtcgtgcgtc cgatgatccg ggtgctgggg
     8461 ctgacgatgt cggccttcaa cgcgtgggtg ctggtcaagt cgctcgagac catggatctg
     8521 aggttgacca agcaagctca gggcgcgctc gcggtagcgg aatggctgga ggcgaatccc
     8581 cttgtctccc aggtgtttta ccccggcttg gcgtcgcacc cgcaggccga gttgatcgcg
     8641 cggcagatga ccggccacgg cacggtggtc acgttcacgc tcgacaccga ccgcgccggg
     8701 gccttccggg cgctggatgc gctggagatc gtcgacatct ccaacaacct cggcgacacc
     8761 aaatccctcg ccacccaccc cgccacgacc acgcactcga agatccccga ggcggagcgc
     8821 ctcgccctcg gcatcaccga cggcaccatc cgcctgtccg tcggactgga gcacccggac
     8881 gacctgatcg ccgacctcga gcgcgccttc gccgccgtgc gctgacgcct gatcctggac
     8941 tccctcgggt agcgcgaact gcctgggccc gcgcggactg ccggagaaat atgtgccgtt
     9001 gttcgccgga tccgaggcgg ttcgctcgat cgcaggcagt tcacggtcgc acgccgggat
     9061 aagcgcccga cgcggcccgg tttatcccca actgctgaac gactgggcgt tttccacagc
     9121 ctcacgggcc aggtctggca tcgacatcgg catcgagcga cgctgaccgg atggacagac
     9181 gactggacgc ggtgacggcg gcgacgggtg gggtcttcac caccaaggac gctgcggcgg
     9241 tagggatccg gcacgaggag atcgcgcaca tggtgcgccg caaggaggtc atccgcgtgc
     9301 ggcgcggcgc gttcgtctcc ggccaggtat atcgcgatgc cgacgtcgac gagcgctatc
     9361 ggctccaggt gatggccgcg ctgcggttgc gtccgaccga tgcggccgcg gcgcacgcag
     9421 cgttggcact gcggcgcctc ccgacctggg gagcagacct gcggcggatc gacctggccg
     9481 gagccgtcaa acatcacgag agcggcgccg gcctgcgagt gcgcccgcgg ggcgacctcg
     9541 aggtcgtgac tatcgggggc cgggccagcg tgcgtatcgc ggatgcgttg gtgcagcacg
     9601 ccgcgcagag cgggatcgtc gccggcgtgg tcgcgatgga cgccgccgtg cacgccaaac
     9661 tcgtgaccgt cgcgagcatc gcggaggcga ttgagcgtat gccgcgcagc gggcggcggc
     9721 gggccagggc ggcggcgacc ctcatcgatc cggcctgcga gtcggtgggt gagtcccgca
     9781 cccggctact gctgctcgac ttcggtctcg cgttcgagtc gcaggccagg ctgtatgagc
     9841 gtcgcaccct gatcgggcgg gtggacttcc tcgtcgagga gcgggtcgtc gtcgagttcg
     9901 acggactggt caagtacagc ggcctcgacg gcaaagaggt gctcgccgcg gagaaggccc
     9961 gcgagagtcg gctcgtcgct ctcggatacg aggtggtgcg cctgacctgg cgcgaactcg
    10021 acgaccccgt cggcgtcatc gctcggatcc gcgacgccct cgcccgcgcc ttgacccggc
    10081 ccgctccacc gcctcgaccc ccggaacccc gaaccgcctc ggaagccgcg aacagcctcg
    10141 tttagcccga actccctggc acatatctgc cggagtccgg gctaagccag gcag
//
LOCUS       DAOPJG010000124        10114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_6967_length_10114_cov_7.725718, whole genome shotgun sequence.
ACCESSION   DAOPJG010000124 DAOPJG010000000
VERSION     DAOPJG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6967_length_10114_cov_7.725718"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKU75_11330"
     CDS             complement(<1..190)
                     /locus_tag="PKU75_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81517.1"
                     /translation="MKQTLHEWRQIGRSRDAVILLVAAIGSLVTDAMAMVALLLRVHA
                     QSPASWWPRSFSACRRGST"
     gene            243..815
                     /locus_tag="PKU75_11335"
     CDS             243..815
                     /locus_tag="PKU75_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF81518.1"
                     /translation="MPTSRTTARTELRIEDPRALRALAHPARQRIISELYAGETLTAT
                     EAAKLVGLTPSATSYHLRALEKWGVAVRDAPGDDGRERPWRSAADSITVLPTAHRRAG
                     LDASRTSVHAWLGDLEDGLERLTASVAQGREQGMTSRGRLWLTPDEERQLRERLGDAV
                     ADLRGRTRRDHPAGATPYDVFALILPADES"
     gene            877..1563
                     /locus_tag="PKU75_11340"
     CDS             877..1563
                     /locus_tag="PKU75_11340"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HPF81519.1"
                     /translation="MSTPTVRQRTWLPLVVVAVGVLTLAVLFAGALSSFRDALFGEDA
                     PPAPGDTTLLEIRKTAELRAATGSYSVPVYYGYDKDKGILSEIVPDVIDANSGVAIYQ
                     GSIDALIDLKSLQDNNIKADKEAGTLRITVPAPALTTPKVDLGKSRIVAQQRGIGTRV
                     GEFFDDAPLEQRQALDQAAVAALQQAAGESGLQATARENGRDFLTALGHQMGFTTVTV
                     IYADEPGALQ"
     gene            1631..2290
                     /locus_tag="PKU75_11345"
     CDS             1631..2290
                     /locus_tag="PKU75_11345"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPF81520.1"
                     /translation="MSMSPSDCTVHGAVTAVSGLRERKKARTTQQLVDAAYALLAEGG
                     FEAMTAEAIADRAGVSRRTFFNYFPSLEAVLVRGVTDLLDEVCGALEDLPADEPLLEG
                     LMQLASSPAQEALLHRVAVLGALGTAAPHTKTVLHTAMLDWLEWLIDFVRSRVPAGSD
                     DLYAVSLATAIVSAAQAAIIRWAEQTGADLSPSGVAAFRATLATSMNYFRRGFDAAPI
                     G"
     gene            2307..4865
                     /locus_tag="PKU75_11350"
     CDS             2307..4865
                     /locus_tag="PKU75_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPF81521.1"
                     /translation="MARILYRIGRWCAEHAKSVVALWLALLVLVGGAAASFGTPMTSK
                     FSLPDSEFQSVLDKLGAEIPEFRGGFGTVVFESETGAFTASQKTAIEKTIASWKNVDH
                     VTAVTDPFAAQQQLDESRTELDAAEKKLTDGQKDLETGRAKLDKAGAQVAQGEGLIKL
                     VARKDPNDPSLPALRKQVADGKAQLAEGEKEWEAGKADLEAGWQRYNVGKAQIDSLGG
                     MHFVTSDGRYALTQIQFDDDVNSVPYEARSQIPERAGDLAAAGVKANYSSEIVQEISL
                     VGPGEIIGLTIALIVLVAMLGTLIAAGLPLVGAIMGVGVGLAGAVAASHFFDMHQMTP
                     TLALMLGLAVGIDYALFIVNRHRSMYLHGTPLRESIGRAVGTSGSAVTFAGMTVVIAL
                     AALVLSGIPILAQMGLVAAGTVAVAVLVAVTLTPALLSLIGTRVASRRLWRANGYSTP
                     ADDSTRVIPGDEHEEEHGGWYVRLTTGHPWLTILAVVSAVVVLALPALSIRLGLPDGS
                     SEPKGSSAYTTYHIIDEHFGKGMNGPVLAVATMDSAQPTAQQAQDAAAELAIALADSA
                     GVDGAAVAGMSDDHKTIALQVIPQSGPADEATLDTVHNLQDSAAHLGEITGTTVGFTG
                     QTVANIEISEKLSAALPGYLLTVVGLSLVILLMVFRSLVVPLVATAGFLLSVAAAFGV
                     TVAVYQWGWLSDLFGVSTPGPILSFMPIILIGVLFGLAMDYQMFLVTGMREAWAHGED
                     AQRAVKSGFLHGAKVVTAAAIIMASVFGGFVFSHLAMIRPIGLGLAVGVIVDAWLVRM
                     TLTPAIMQLLGERAWTLPRWLDRLIPNVDVEGASLEGHTGPTPGRPTATQPAGA"
     gene            5314..5661
                     /locus_tag="PKU75_11355"
                     /pseudo
     CDS             5314..5661
                     /locus_tag="PKU75_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            complement(5671..6138)
                     /locus_tag="PKU75_11360"
     CDS             complement(5671..6138)
                     /locus_tag="PKU75_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF81522.1"
                     /translation="MAINGLGPEDETPVFVISVAAQLAGMHAQTLRQYDRLGLVTPTR
                     TPGGGRRYSARDVARLREVQRLSQEEGVSLAGIQRILDLENHVEALRARVEELTFAFQ
                     RAEMASGRRVFAVGSAGETIVLRPGERPPHLGSQALVRWDPFAPRPRTRAGQR"
     gene            complement(6144..7172)
                     /locus_tag="PKU75_11365"
     CDS             complement(6144..7172)
                     /locus_tag="PKU75_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPF81523.1"
                     /translation="MASQDWFEKDFYAILGVPSDADDAAIKKAYRKLARQHHPDKNAG
                     DPAAEQKFKDITEAHSVLSDKKQRQEYDAVRQMARGGARFTAGGPGGGAGAEGFEDIF
                     SSMFGGQGGNVRFSTRGGGGQPDLNDILGGLFGQQGGFGQGSPYGDRGGFGAPRGPQR
                     GPDIQAKTTLSFRDAVEGSTVTLSGLDGQRITTRIPAGVKDGQKIKLRGKGGDGDPGA
                     AKGDLILTVSVEKHPVFGRDGDNLTIDLPVTFAEAALGATVAVPTLDGHAVKVKIAPG
                     TPSGRVLRVKGRGVKTKSHTGDLLAKVAILVPQRLSDEARAAIETLRAQEEGADPRAD
                     LFREAAKD"
     gene            complement(7191..7769)
                     /gene="grpE"
                     /locus_tag="PKU75_11370"
     CDS             complement(7191..7769)
                     /gene="grpE"
                     /locus_tag="PKU75_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPF81524.1"
                     /translation="MTDSPITPNGGQPVEADAVNTEAANDAAAPADSAPAESAGAPPP
                     EAVHPDTVLAAERLNDLQRLNAEYVNYKRRVDRDRPLIQERAIADVLEALLPVMDDIQ
                     GARDHGDLTEGPFAAISDKLENTLGRFGFERFGVVGEAFDPEQHEALMHLPDADLPAE
                     ATTTTIVQVLQPGYRAGTKVIRPARVAVADPQ"
     gene            complement(7766..9661)
                     /gene="dnaK"
                     /locus_tag="PKU75_11375"
     CDS             complement(7766..9661)
                     /gene="dnaK"
                     /locus_tag="PKU75_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPF81525.1"
                     /translation="MARAVGIDLGTTNSAVAVLEGGEPTIIANAEGGRTTPSVVAFAK
                     NGEVLVGEVAKRQAVTNADRTLRSVKRHMGTDWKTDEIDGKKYTAQEISARILQKLKR
                     DAEAYLGEPVTDAVITVPAYFDDAERQSTKEAGEIAGLNVLRIVNEPTAAALAYGLDK
                     GKEDELILVFDLGGGTFDVSLLEVGKDPEDGFSTIQVKATSGDNRLGGDDWDDRIVQH
                     LLTTVKNTTGVDLSKDKIAMQRLRDAAEQAKKELSSATSTNISLQYLSMSENGPIHLD
                     ETLTRAQFEQMTKDLLDRCKAPFQNVIKDAGIKLEDIDHVVLVGGSTRMPAVSALVKE
                     LAGKEPNKGVNPDEVVALGASLQAGVLKGERKDVLLIDVTPLSLGIETKGGLFTKLIE
                     RNTAIPTKRSEVFSTAEDNQPSVLIQVFQGEREMAQMNKNLGTFELSGIAPAPRGVPQ
                     IEVTFDIDANGIVHVTAKDRGTGKEQKITISGGSALSKEEIDRMVKDAEAHAADDAKR
                     REETESRNTAEQLVYSTEKFLADSGDKLTDDLRKPVDEALADLKEAIKPESTASVEDI
                     NAKVTKLNEESQKMGAALYAASAEQGESPNMGGGESTDSAQGTTPADDSDVVDAEVVE
                     DDEDDKK"
BASE COUNT         1490 a         3443 c         3443 g         1738 t
ORIGIN      
        1 ccgtcgaccc gcggcggcag gcggagaagg agcgcggcca ccacgaggcc ggcgactgcg
       61 cgtgcacgcg cagcagaagc gcaaccatgg ccatcgcgtc cgtcacgagc gacccgatcg
      121 cggccacgag caggatcacc gcgtcccgag aacgaccgat ctgtctccat tcgtgaagcg
      181 tttgtttcat ggttatgaaa gtatcacttc acagttatga agcaactatt acgatgggcc
      241 gtatgccgac ctcccgcacg accgctcgca ccgagctgcg gatcgaggac ccgcgcgccc
      301 tgcgcgccct ggcccacccg gcccggcagc ggatcatctc cgagctgtat gccggcgaga
      361 cgctcacggc gacggaggcg gcgaagctcg tggggctgac gccgtcggcg acgagctatc
      421 acctgcgcgc cctcgagaaa tggggtgtcg cggtgcgcga tgcgcctggc gacgacggcc
      481 gggaacgacc gtggcgttcg gccgccgact cgatcactgt gctgcccacg gcccatcggc
      541 gcgcggggct cgatgcgagt cggacctcgg tccacgcgtg gctcggggac ctcgaggacg
      601 ggctcgagcg gctcacagcc tcggtcgcgc agggccgcga gcagggaatg acgtctcgag
      661 gccgcctgtg gctgacgccg gacgaggagc ggcaactgcg ggaacgcctc ggggacgccg
      721 tggcggatct gcggggccgt acgcggcgtg atcaccccgc cggcgcgacc ccgtatgacg
      781 tctttgccct catcctcccc gcagacgaat cgtgacccga tctgcggccc tccctcccgc
      841 cacggatggc agagtggccc gcatgtgggg tttgcgatga gcacaccgac cgtgcgccag
      901 cgcacgtggc tgcccctggt ggtcgtggcc gtgggcgtgc tgaccctggc cgtgctcttc
      961 gccggcgcgt tgagcagctt tcgcgatgcc ctcttcggcg aggacgcgcc accggcgcct
     1021 ggcgacacga ccctgctcga gatccgcaag acagcggagt tgcgggccgc gaccgggtcc
     1081 tattcggtgc ccgtctatta cggctacgac aaggacaagg gcatcttgtc cgaaatcgtg
     1141 cccgatgtca tcgacgcaaa ctccggtgtt gcgatctatc aggggagcat cgacgcgctc
     1201 atcgacctga agtcgttgca ggacaataac atcaaggccg acaaagaggc cggcacgctg
     1261 aggatcacgg tgcccgcccc ggccctgacg acgcccaagg tggacctcgg caagtcgcgg
     1321 atcgtcgccc agcaacgcgg catcggcacg cgcgtcgggg agttcttcga cgacgcaccg
     1381 ctcgagcagc ggcaggcgct cgaccaggcc gcggtggcgg ccttgcagca ggccgccggg
     1441 gagtcggggt tgcaggcgac cgcccgcgag aacggccgcg acttcctgac cgcactcggg
     1501 catcagatgg gtttcaccac cgtcacggtc atctatgcgg acgagcctgg ggcgctccag
     1561 taacgcacgt ctcatagctg cactcggggg caaatttgca ctgagtgcat gatggtgtca
     1621 cagtggagac atgagcatgt ctcccagcga ctgcaccgtc cacggcgccg tcacggcggt
     1681 gagcggcctg cgggagcgca agaaggcccg cacgacccag caactcgtcg acgcggccta
     1741 cgcgctgctc gccgagggtg ggttcgaggc gatgaccgcc gaggccatcg ccgaccgcgc
     1801 cggcgtctcg cgccgcacct tcttcaacta cttcccttcc ctcgaggcgg tcctcgtccg
     1861 cggcgtcacc gacctgctcg acgaggtgtg cggggctctc gaggacttgc cggccgacga
     1921 gccactcctg gagggcctga tgcagctggc gtcctcgccg gcccaggaag ccctcctgca
     1981 ccgcgtcgcc gtcctggggg cgctcggcac tgccgcaccg cataccaaga ccgtgctgca
     2041 caccgccatg ctcgactggc tggagtggct catcgacttc gtccgctcgc gggtccccgc
     2101 gggcagcgat gacctgtatg ccgtctccct cgccaccgcg atcgtctcgg ccgcccaggc
     2161 cgcgatcatc cgctgggccg agcagaccgg cgccgacctt tccccctcgg gtgtggctgc
     2221 cttccgggcc acgctcgcca ccagcatgaa ctacttccgt cgcgggttcg acgccgcgcc
     2281 gatcggctga ggaggccgac tcccacatgg cacgaatcct ctatcgaatc gggcgttggt
     2341 gcgccgagca cgccaagtcc gtcgtcgccc tctggctcgc tctgctcgtc ctcgtgggcg
     2401 gcgcggcggc gagcttcggg acgccgatga cgagcaaatt ctcgttgccc gacagcgagt
     2461 tccagtccgt gctcgacaag ctgggcgcgg agatccccga gttccgcggc ggcttcggca
     2521 ccgtggtctt cgagagcgag accggcgcgt tcactgcttc gcagaagacg gcaatcgaga
     2581 agacgatcgc ctcgtggaag aacgtcgatc acgtcaccgc cgtgaccgat ccatttgccg
     2641 cccaacaaca actcgacgaa tcgcgcaccg aactcgacgc cgccgagaag aagctgacgg
     2701 acgggcagaa ggatctcgaa acgggacgtg ccaagctcga caaagccggc gcccaggtcg
     2761 cccagggcga gggcctgatc aagctcgtcg cccggaagga cccgaacgac cccagcctgc
     2821 cagccctgcg caagcaggtc gccgacggca aagcgcagct cgccgagggt gagaaggagt
     2881 gggaggccgg caaggccgac ctcgaggccg gctggcaacg ctacaacgtc ggcaaggccc
     2941 agatcgactc actcggcggc atgcacttcg tcacctccga cgggcgctac gccctgaccc
     3001 agatccagtt cgacgacgac gtcaattccg ttccgtatga ggcccgttcg cagatccccg
     3061 agcgtgccgg cgatctcgcc gcggcgggcg tcaaggcaaa ctactcaagc gagatcgtgc
     3121 aggagatctc gctcgtcggg cccggcgaga tcatcggtct caccatcgcg ctcatcgtcc
     3181 ttgtcgcgat gctcggcacc ctcatcgcgg ccggtctgcc gctcgtcggc gccatcatgg
     3241 gtgtgggtgt cggcctcgcc ggcgcggtgg ccgcctcaca cttcttcgac atgcaccaga
     3301 tgacgccgac cctggccctg atgctcggcc tcgccgtcgg gatcgactac gccctgttca
     3361 tcgtcaaccg gcaccggtcg atgtatctgc acggcacccc cctgcgcgaa tcgatcggac
     3421 gggccgtcgg gacctccggc agcgccgtga cgttcgccgg tatgaccgtg gtcatcgccc
     3481 tcgcggccct cgtcctgtcg ggcatcccca tcctcgccca gatgggcctg gtcgccgccg
     3541 gcaccgtcgc ggtcgcggtg ctcgtcgccg tcaccctgac gcccgcgctg ctcagcctga
     3601 tcggcacccg ggtggcctca cgccggctgt ggcgcgccaa cggatactcg acgccggcgg
     3661 acgactcgac gcgcgtcata ccgggcgacg agcacgagga ggagcacggc ggatggtatg
     3721 tccgtctgac gaccggtcac ccctggctga ccatcctcgc cgtggtctcc gcagtcgtcg
     3781 tgctcgcatt gcccgccctc tccatccgac tcggcctccc cgacgggtcg agtgagccga
     3841 agggcagctc cgcctacacg acctatcaca tcatcgacga gcacttcggc aagggcatga
     3901 acggcccggt gctggccgtc gcgacgatgg actcggcgca gcccaccgcg cagcaggcgc
     3961 aggacgcggc cgccgaactc gccatcgccc ttgctgactc cgccggcgtg gacggcgccg
     4021 ccgtcgccgg catgtcggac gaccacaaaa ccattgcgct gcaggtgatt ccccagagcg
     4081 ggccggccga tgaggccacg ctcgacaccg tgcacaacct gcaggacagc gccgcccacc
     4141 tgggcgagat caccggcacg acggttgggt tcacgggcca gacggtcgcc aatatcgaga
     4201 tctccgagaa gctgagcgcc gccttgcccg gctatctgct caccgtcgtc ggcctctcgc
     4261 tcgtcatcct gctcatggtg ttccgctcgc tcgtcgtgcc gttggtcgcg acggccggat
     4321 tcctgctgtc cgtggcggcg gctttcggcg tgacggtcgc cgtctaccaa tgggggtggc
     4381 tctccgacct cttcggcgtc agcacgcccg gaccgatcct gtccttcatg cccatcatcc
     4441 tcatcggcgt gctcttcggc ctggcgatgg actaccagat gttcctcgtc accggcatgc
     4501 gcgaggcgtg ggcgcacggc gaggacgccc agcgcgcggt gaagtcgggc ttcctccacg
     4561 gggccaaggt cgtcacggcc gcggccatca tcatggcgtc ggtcttcggc ggcttcgtct
     4621 tctcccacct ggcgatgatc cggcccatcg gcctcggcct cgccgtcggc gtgatcgtcg
     4681 acgcctggct ggtgcggatg acgctgaccc cggccatcat gcaactgctc ggcgaacgcg
     4741 cctggaccct cccgcgctgg ctggaccgcc tcataccgaa tgtcgacgtc gagggtgcca
     4801 gcctcgaagg ccacaccggc ccgacgcccg gccgacccac ggcgacgcaa cccgctggcg
     4861 cctagactct cggatccttc agcacggcga cgaacgttgt acgtatccac gaccagttgc
     4921 cctcccggat agcggcacgg tacggaatgc tccccgttgc ccttgcgcgg cgtccccgac
     4981 ctagcgtcgg cggcacctca ccgccggcca cggcgtgact gagtccggag ccttctgcaa
     5041 cacctgttgc tgtatgacgt tcccgcgcgg caccttctgt tgctgctgcg gctgaggccg
     5101 acccgtggcg gatgcgctgc gctcccgcag ggctgtacgc atccgccacg accggccgcc
     5161 aacggtcccg taccactgcg catcgggagt gcttgcccct gcgccggggc tgcgatctag
     5221 cgtcgaagcg ccaaccagtg aaaggacgcg gcccatggac acccagacga cggtcgacgt
     5281 cgatgacctg ccgatgaact ccttcgagct cgcaaggcca tcgccgatgt ccagttgagt
     5341 ggagtgacca tccgcgcggg cgacatcgtg atccccgcag tcggcgccgc caaccgcgac
     5401 cccgaggtct tcccccatcc ggacaccccc gacgtggatc gcgttgggcc gcgccatctt
     5461 tcgttcgggg ccggagctca cgcgtgcatc ggtatggcgc tggcccggct ggaactgctg
     5521 gtgacgctgc gcgagctcag ccgcgcgctg ccggatctga cactctgctg cgcggtggac
     5581 gagctgagtc ggtcgcaccc ggagctgccg gtgaacccga tcgagaggat ccccctgcgc
     5641 ctgtcggcgg tcacccactg acgcttccgg tcagcgctgg ccggcgcggg tgcggggccg
     5701 gggggcgaag ggatcccagc gcaccaaggc ctgcgagccg aggtgcggag ggcgctcgcc
     5761 gggacggagc acgatggtct ctccggcaga gccaacagcg aaaacccttc gcccagaggc
     5821 catttcggca cgctggaacg cgaaggtcaa ctcttcgact cgcgctcgga gagcttcgac
     5881 gtggttctcg aggtcaagga tccgttggat accggcgagg ctgacgccct cctcctgcga
     5941 gagtcgctgc acctcccgca gccgggcgac gtcccgcgcc gaatagcgcc ggccgccgcc
     6001 cggggtgcgg gtgggggtga ccagcccgag cctgtcgtac tggcggaggg tctgcgcgtg
     6061 cataccggcg agctgggccg cgacggagat gacgaagacc ggagtctcgt cttcggggcc
     6121 gagcccgttg atcgccatga cggtcagtcc ttcgccgctt cgcggaagag gtcggcccgc
     6181 gggtcggcgc cctcttcctg agcgcgcagc gtctcgatcg cagctcgagc ctcgtcggaa
     6241 agccgttgcg gcacaaggat ggcgaccttg gcgagcaagt ctcctgtgtg ggacttggtc
     6301 ttgacgccgc gacctttgac tcgcaggacg cggcccgacg gagtccccgg agcgatcttg
     6361 accttgacgg cgtgaccgtc gagcgtcggc accgcaaccg ttgcgcccaa ggcggcttcg
     6421 gcgaaggtga ccggcaggtc gatggtcagg ttgtcgccgt cgcgtccgaa gacggggtgc
     6481 ttctcgaccg agacggtgag gatcaagtcg cccttggcgg cgccgggatc accgtcgccg
     6541 cccttgccgc gcagcttgat cttctggcca tccttgacac cggccgggat gcgcgtggtg
     6601 atgcgctgcc cgtccaggcc gctgagggtg accgtcgagc cctcgaccgc gtcgcggaag
     6661 ctcagggtcg tcttggcctg gatgtccggg ccccgctgcg gcccgcgcgg ggcgccgaag
     6721 ccgccgcgat cgccatacgg tgaaccctgc ccgaacccac cctgctggcc gaacagacca
     6781 ccgagaatgt cgttcaggtc cggctggccg ccgcccccgc gggtgctgaa gcggacgttg
     6841 ccgccctgcc cgccgaacat cgaggagaag atgtcctcga agccctcggc gccggcgccc
     6901 ccgccggggc cgccggcagt gaatcgggct ccgccacggg ccatttggcg aacggcgtca
     6961 tactcctgac gctgcttctt gtccgacagc accgagtggg cctcggtgat gtccttgaac
     7021 ttctgctcgg cggcgggatc cccggcgttc ttgtccgggt ggtgctgccg ggcgagcttg
     7081 cgataggcct tcttgatggc cgcgtcgtcg gcgtcgctgg ggacgccgag gatcgcgtag
     7141 aagtccttct cgaaccagtc ttggctagcc atgttggcgt cctcccttcg ttactgcgga
     7201 tcggcgacgg ccacgcgggc cggacggatg accttggtgc cggcacgata gccgggctgg
     7261 agcacctgga cgatggtggt cgtcgtggct tcggccggca ggtcggcgtc gggcaggtgc
     7321 ataagggcct cgtgctgctc ggggtcgaag gcttcaccca cgacgccgaa acgctcgaaa
     7381 ccgaagcgcc ccaaggtgtt ttcgagtttg tcggagatgg cggcgaacgg accctcggtg
     7441 aggtcaccat ggtcgcgcgc gccctggatg tcgtccatca ccgggagcag cgcctcgagg
     7501 acgtcggcga tcgcccgctc ctggatgagc ggccggtcac ggtcgacgcg acgcttgtag
     7561 ttgacgtatt cggcgttgag ccgctgcagg tcgttcagac gctcggcggc caggacggtg
     7621 tccgggtgga cggcctccgg cgggggcgcg cccgcggact cggccggagc cgaatccgca
     7681 ggcgcggcgg catcattcgc cgcttccgtg ttcaccgcgt ccgcctcgac cggctgcccg
     7741 ccgttcgggg tgatcgggga atcggtcact tcttgtcgtc ctcgtcgtcc tcgacgacct
     7801 cggcgtccac gacgtcggag tcgtcggcgg gggtcgtgcc ctgcgcggag tcggtggact
     7861 cgccgccacc catgttgggc gactcaccct gctcggcgga cgcggcatac agcgcggcgc
     7921 ccatcttctg ggactcctcg ttgagcttgg tgaccttggc gttgatgtcc tcgaccgagg
     7981 ccgtcgactc gggcttgatg gcctccttaa ggtcggccaa cgcctcatcg accggcttgc
     8041 gcaggtcgtc ggtcagtttg tcgccgctgt cggcgaggaa tttctcggtc gagtagacca
     8101 gctgctcggc ggtgttgcgc gactcggtct cctcgcggcg cttggcgtcg tcggcggcgt
     8161 gcgcctcggc gtccttgacc atgcggtcga tctcctcctt ggacagggca gacccgccgg
     8221 agatggtgat cttctgctcc ttgccggtgc cgcggtcctt ggccgtgacg tggacgatgc
     8281 cgttggcgtc gatgtcgaag gtgacctcga tctggggcac gccacgcgga gccggcgcga
     8341 tgccggacag ttcgaaggtg ccgaggttct tgttcatctg ggccatctcg cgctcgccct
     8401 ggaagacctg gatcagcacg gacggctggt tgtcttcagc ggtggagaag acctccgaac
     8461 gcttggtcgg gatggcggtg ttgcgctcga tgagcttggt gaacaggcca cccttggtct
     8521 cgatgccgag gctgagcggg gtgacgtcga tgagcaggac gtccttgcgc tcacccttga
     8581 ggacaccggc ttggagggag gcgccgaggg cgacgacctc gtcggggttg acacccttgt
     8641 tgggctcctt gccggcgagc tccttcacga gggcggaaac ggccggcata cgggtcgaac
     8701 cacccaccag gacaacgtga tcgatgtcct cgagcttgat gcccgcgtcc ttgatgacgt
     8761 tctggaaggg ggccttgcag cggtcgagga ggtccttggt catctgctcg aactgggccc
     8821 gggtgagggt ctcgtcgagg tggatggggc cgttctcgct catggagagg tactgcaggg
     8881 agatattggt gctggtcgcg gacgacagct ccttcttggc ctgctcggcg gcgtcgcgca
     8941 gacgctgcat ggcgatcttg tccttggaca ggtcgacacc ggtggtgttc ttcaccgtgg
     9001 tgagcaggtg ctggacgatc cggtcgtccc agtcgtcgcc accaaggcgg ttgtcaccgc
     9061 tggtcgcctt gacctggatg gtggagaagc cgtcctcggg gtccttgccg acctcgagga
     9121 gggagacgtc gaaggtgcca ccgccgaggt cgaagacgag gatcagctcg tcctccttgc
     9181 ccttgtcgag gccgtaggcc aacgcggccg cggtgggctc gttgacgatg cgcaggacgt
     9241 tcaggcccgc gatctcgccg gcctccttgg tggactggcg ctcggcgtcg tcgaagtatg
     9301 ccgggacggt gatgaccgcg tcggtcacgg gctcacccag gtaggcctcg gcgtcgcgct
     9361 tgagcttctg aaggatgcgc gccgagatct cctgcgcggt gtacttcttg ccgtcgatct
     9421 cgtcggtctt ccagtcggtg cccatgtggc gcttgaccga gcggagggtg cggtcggcgt
     9481 tggtgacggc ctgccgcttg gcgacctcac cgacgaggac ctcgccgttc ttggcgaagg
     9541 cgacgacgga cggggtggtg cgaccgccct cggcgttcgc gatgatggtg ggctcgccgc
     9601 cttcgaggac ggcgacagca gagttggtgg ttccgaggtc gataccgact gcacgggcca
     9661 tgtggtgatg ctccttgtgt gtgagagttg agctgcttgc actcaagtta aggaccgtag
     9721 gcggcttgcg caacttgagg tccagaaact tgcgtctgct tgactcaacc cttgacaccc
     9781 cggcggtgtt cccgattccc ggagaaagtc ggcgaccatt cctcctgtag gttctcgtcg
     9841 aacttggccg agtctcgctt tcttggccgc ggcgtgtccg cgtctcgatt tcgtggccgc
     9901 gggtggacgc tgatggtgtg aggcgtgcca gcgtcgatgt ggccgtccga tacacgagcc
     9961 atgtggggtc ggaggtcgaa acgaccttgg ctcacgtcga ggggcgcgac gtggcgagcg
    10021 gccgtccggt tcggcggttc gggatgcatg ccgggatggg gcactatccg ggctggtggt
    10081 ggtgatcgac catgcaggac ctggtaggcc acga
//
LOCUS       DAOPJG010000125        10081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7004_length_10081_cov_8.240874, whole genome shotgun sequence.
ACCESSION   DAOPJG010000125 DAOPJG010000000
VERSION     DAOPJG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7004_length_10081_cov_8.240874"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKU75_11380"
     CDS             <1..622
                     /locus_tag="PKU75_11380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81526.1"
                     /translation="TVTPATAARWDDVVATFGTKGDPSWCWCQYFATTGASYEKSAAA
                     NKDALHGQVTARPSTTRPAGLLAYRGREAVGWVQLGPIAAFPRVTGKARRTAALVSPS
                     GDGAQPENLWRVACFVVPPKQRRPGVARALLDGAVAYARDRGADALEAHPVDTGGKRV
                     PGADLFHGVLSTVVAAGFEVIGRTATSRPIVRLDLTVPQASRPQAR"
     gene            complement(610..>1788)
                     /locus_tag="PKU75_11385"
     CDS             complement(610..>1788)
                     /locus_tag="PKU75_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81527.1"
                     /translation="AAAADRHAGSLFGHRTHTAGGTACGGVWLCGGMAASHTKKTALS
                     TTTSTTSKTVAANRGGPKLDRKTREELLEAHVAFELDRWHGEAFAAGVREEVAALQAW
                     LGEVTVGDLLPGADTAHTLATALADVDLTAAAADLATDVLRVIRAALAASEATVGDVA
                     AARDLHGIVDLVTHLDELRGTVIGAVTDSPAYHELVAHVLYHGVKAFVLNENIVARKL
                     PGAQSLIRMGQRGLATAAPGLESGVDRQLRRFVQNQIGDTLIDSKRFLDRTLSGESGH
                     ELLDTTLSTVGPKRLAEFACLITDDDIEAVGDAAAPVARGILRSGVVAQATAAAVEQV
                     LAAYADQPVAPLLADLGLDLDELADRFVDLVAPALEHAHASGYTEARIRARLSAFYLA
                     "
     assembly_gap    1789..1888
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1952..2200)
                     /locus_tag="PKU75_11390"
     CDS             complement(1952..2200)
                     /locus_tag="PKU75_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81528.1"
                     /translation="MTAGESAARAAVAASTLAAAHRRDHHHTSECCVPHCVETVHLGG
                     KAAMVCHDCGTDSGFLDNRAVAVLCREHAEETREGSAA"
     gene            complement(2303..5716)
                     /locus_tag="PKU75_11395"
     CDS             complement(2303..5716)
                     /locus_tag="PKU75_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81529.1"
                     /translation="MNGTQPASSTDLLAAVNVRLDLLGQPKVEAVEPGQAVADDLVAP
                     ILKRQRELSRRLGDRLPPVDERIEAFLTAYLHGVTPIPHLPRRTLSLDLPGLARTLSL
                     PVDSDEYSSGLITSYRLANGVLHNPRSDRRTTAGVFHIAEGGLPIPDDKKAVPREVFA
                     ALLDRALTPPAAALELPITSTQEEKAACFVSLLLRPLVTVGVPGHTHERRMETRFIVP
                     GSMVANLDFVEGIFGNAGDPYLPENDAALDPLGWTGHTGLVLLAPHLISATKQSLGLP
                     HKDDATERQIRDGMCWTDPAERYNDGQAFKVCARDERGVMVTIIADNYFGYCKKEVKT
                     QISMSANLFGGSEEEHSGGAIVLPSWDEGQEFVNTYAEGAPSVAEVTARDPRAWTPDE
                     AGWARCVDHEDIVLVPTGAKFSLQERTVSWAGEKDTVTIPFRGDTTYLDTSGFRVVMK
                     QVRREPVQWTIIGTAATSTVCHKPSTVSGGGKSEISKSIADAITIGQAFVADFDADMD
                     AVEQIVSRDYSERYANPEWNGKDHRSVLGPERSTGSVIKLFTPKAEFSDEHNAFIRAI
                     PPHVLELVYVIKRAYRSEWGADWRSHCSVSQVNGRTGNRLRIDGQAVLVDMLRVGFES
                     DGSYRLFSLRPDFAPSVKVQTEDDITASVVAPTSGPGEPSVKYVENCERLLFQRPDDA
                     ITPGYDSTAEADIASPGTFLSNFQPLDVAAATELADDAIAVSQFTEPMRELITDFAAG
                     TLPGDATYMVCSARPRIVDGKPSKNPRYLQVRPDMADPAGTARALTALRIARGLTMDE
                     PMVTGVDIVAAGRRNNPPESGVPALCAFGPLHYLELPELLMEFISSMTGKSPSTTGAG
                     SEGAMTKAPFNALPTTYDLNAAFLSFALTGYDGWISGAGYVGPEVRVDHDVSLLIPEL
                     FARMSPAERSAAALIEAGYLEPVADVVLDDGRVVAASRLGYRITEKFASTFLGRIFMH
                     PDAIFTEAILRPETQDRQVFVDSVDVMVSTHERVARAYLKDGTIESACPPVRALLEIM
                     ADGISADGSTLEDPAFRAMFTREAVLASSWYAARLDAAQAQLVARSEAGIAAIEGLLA
                     EDGDASVSERMALRERLSDVTAARDDAASPEARARLVGTLGRQPSFRSA"
     gene            complement(5871..7259)
                     /locus_tag="PKU75_11400"
     CDS             complement(5871..7259)
                     /locus_tag="PKU75_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010525736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPF81530.1"
                     /translation="MSYDVVEAGIVQLAAALARGEVTSAELVDAYLARIAAYDHAGPH
                     LNSVVVLDPNARAEAEESDRRRTRGEPLGPLDGIPYTAKDSYSATGLTVAGGSPAFAH
                     LVAQRDSYAVGRLRGAGAILLGLTNMPPMANGGMQRGLYGRAESPYNPAYLTAPFASG
                     SSNGSGTATAASLAAFGLGEETWSSGRGPASNNGLCAYTPSRGVISIRGNWPLVPTDL
                     LWALVEDVRAAGAEVVLTDFPIVSNYEADRPGAATIRTRGLVSPVYLEREVQDLAAWS
                     WDDFLHANGDPGLHRLADIDGAQIFPPPPGALKDRYHDFGDDIALFPGYARAHPLVPL
                     REHTWVAEGIAGLDRTRELDLDAWLDSERLDAVIFPAVADVGPADADVDPASADLAWR
                     NGVWVANGNLVPRHFGLPTVTIAMGVMRDISMPVGLTFAGRPYADTALLSYAAATEVL
                     CPRRSPHPGHPH"
     gene            7416..8837
                     /locus_tag="PKU75_11405"
     CDS             7416..8837
                     /locus_tag="PKU75_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81531.1"
                     /translation="MTEGPEGSPADGPDAPGHSEHHAARLPPGITPGPQTLRAFRAVL
                     ANTLVANVTTSYLWFALTFWAYLETKSVLATSILGGSYMLLIAVGGIFFGTLVDHHRK
                     HAVMVASSAITLVMFMLAGALYLLFPTQRLVDWNGPVFWAFAGVILLGGVVESLRNIA
                     LSTTVTLLVPDGQRDKANGQVGTVQGIAFMITSVFSGLSIGLLGMGWTLGLAIGLTAL
                     ALGHLLTITIPEQRPPRDEGARPPAFDIRGAIGAVRAVPGLGALILFSCFNNLVGGVY
                     MALMDPYGLTLFSVESWGIVLGVTSFGFVLGGLWVARFGLGEKPLRSLMLVNVVVALI
                     GIFFTVREVWWVYAGGILLFMCVIPIAEAAEQTILQRVVPFAKQGRVFGFAQSVEVAA
                     SPISAFLIGPIAQYGLIPYMKTAEGQERFGWLVGAGQARGIALVFIASGLLMLVAVAV
                     AMISPPYRVLSRSYQQAEPPPAS"
     gene            8845..>10081
                     /locus_tag="PKU75_11410"
     CDS             8845..>10081
                     /locus_tag="PKU75_11410"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81532.1"
                     /translation="MKADEARDAGKAAGAGLRTGTGLIRDIHGGISHGVYAVVDALAG
                     PAARPIRVVQDTITEGVYAVTGRGLDVGSRLVGAAVAARKARSGADHASLHDGPAAHH
                     VIAIGSGLIDDSLVGQVPSLRPTMHVRERGTRVELTREGVGAAYPEATDEIVVFLHGL
                     VETEAAWRLSAKTRPPYADRLRADLGLTPVMVRYNTGLRVSENGRLFAGLLADLVEAW
                     PVPVRRIALIGHSMGGLIIHSAFAQAEDDPATQWRGLVTDTITLGTPHHGSPLARGAF
                     AATAWLGRSRHTRTAAQVMRARSGSVRDLAHGNILAADWESLDVEELVDGRTHPAPAM
                     GILHRAVGAAWGKAHPDGGHDRLSDFLVPVTSATHAHLAGEARRFTEDDIAVVHGTHH
                     LGLLNSEETYQHLLRWLTPA"
BASE COUNT         1446 a         3369 c         3593 g         1573 t
ORIGIN      
        1 caccgtcacg ccggcgacag cggcccggtg ggacgatgtc gtcgcgactt tcggcaccaa
       61 gggcgacccg tcctggtgct ggtgccaata cttcgccacg accggcgcct cctacgagaa
      121 gtccgccgcg gccaacaagg acgcactaca cggccaggtg accgcccgcc ccagcacgac
      181 gcggcccgcg gggctgctgg cataccgcgg ccgggaagcg gtcggctggg tccaactcgg
      241 accgatcgcc gcctttcctc gggtgaccgg caaggcgcgc cgcaccgcag cgctcgtctc
      301 cccgtccggc gacggcgcgc aacccgaaaa cctttggcgt gtcgcatgtt tcgtcgtccc
      361 gccgaagcag cggcgcccgg gcgtggcgcg cgctctcctc gacggcgctg tcgcctatgc
      421 ccgggatcgg ggcgccgacg cattggaagc ccatccggtc gacaccggcg gcaagcgggt
      481 cccgggagcc gacctctttc acggcgtcct gagcactgtc gtcgccgccg gcttcgaggt
      541 gatcggacgc accgccacgt cacgcccgat cgtgcggctc gacctgacgg tgcctcaggc
      601 cagtaggcct caggccagat agaacgccga gaggcgggcc ctgatccgag cctctgtgta
      661 gccgctcgca tgggcgtgtt ccagggccgg agccacgaga tcgacgaacc ggtcggcgag
      721 ctcgtcgagg tcgaggccga ggtcggcaag cagcggcgcg acgggctggt ccgcgtatgc
      781 cgcgagcacc tgctccactg ccgccgcggt ggcctgcgcg accacgcccg accggaggat
      841 cccgcgcgcc accggcgcag cagcatctcc cacggcctcg atgtcgtcgt ccgtgatcag
      901 gcaggcgaat tcggcgagtc gcttcggtcc gaccgtgctc aaggtggtgt cgagcagttc
      961 gtgccccgat tcgccgctga gggtgcggtc gaggaagcgt ttgctgtcga tcaaggtgtc
     1021 gccgatctgg ttctggacga accggcgcag ctggcggtcg accccgctct ccaacccggg
     1081 ggccgccgtc gccagccctc gctgccccat ccggatcagc gactgcgcgc cgggcagctt
     1141 gcgagcgaca atgttctcgt tgaggacgaa tgccttgacg ccgtgataga gcacgtgggc
     1201 caccagttcg tgataggccg ggctgtccgt caccgcgccg atcactgtgc cgcgcaattc
     1261 gtccaggtgt gtgacgaggt ccacgatccc atgcaggtcc cgggcggccg ccacatcgcc
     1321 gacggtcgcc tcggatgcgg cgagggcagc ccggatcacc cggagcacgt cggtggcgag
     1381 gtcggccgcg gcggcggtca ggtccacgtc tgcgagagcg gtggcgaggg tatgggcagt
     1441 gtccgccccg ggtagcaggt cgcccaccgt cacctcgccg agccaggcct gaagggcagc
     1501 tacttcttcg cgcacgcccg cggcgaacgc ctcgccgtgc cagcgatcca gctcgaatgc
     1561 gacgtgggct tcgagcaact cctcacgggt cttgcggtcc agcttcgggc cgccccgatt
     1621 cgccgcgaca gtcttggacg tcgtggaggt cgtcgtggac agcgcggttt tcttggtgtg
     1681 ggacgcggcc atacccccac acaaccagac cccgccgcac gccgtgccgc cggcggtgtg
     1741 tgtcctgtgt ccgaaaaggc tgccggcatg ccggtcggcc gccgcagcnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg atcaccggct ggtgtgggcc gacgtgctgg
     1921 tgcgcggaaa gggccgctag gcgcggccgg ctcaggccgc ggatccctcg cgggtctcct
     1981 ccgcgtgctc gcggcacagc acggcgacgg cacgattgtc gaggaaaccg gagtcggtgc
     2041 cgcagtcgtg gcacaccatt gctgccttgc cgccgaggtg gaccgtctcg acgcagtgcg
     2101 gcacgcagca ctcgctcgtg tggtgatggt cgcgccggtg tgcagcagcg agagtgctcg
     2161 ctgcgactgc cgcgcgggcc gcggactctc cagccgtcat ggttgtcctc cggtgagtgc
     2221 ctggtgtggc gtgagtcacg ctaatacacg tccgggggtc ccgggaggtt gccacgcacg
     2281 gtttgcggct acctgggcac gcctacgcag agcggaacga cggctgccgc ccgagtgtgc
     2341 cgacgaggcg ggcgcgagcc tccggtgagg cggcatcgtc acgcgcggca gtgacgtcgg
     2401 aaagccgctc ccgcaacgcc atccgctccg agaccgaagc gtcgccgtcc tcggcgagca
     2461 aaccctcgat ggccgcgatg ccggcctccg agcgcgcgac gagctgcgct tgcgcggcgt
     2521 ccaggcgggc ggcataccag ctgctcgcca gcaccgcctc gcgggtgaac atggcgcgga
     2581 aggcgggatc ctccagggtc gatccatcag cggagatgcc gtccgccatg atctctagca
     2641 acgcgcgcac gggcgggcag gcggactcga tcgtgccgtc cttgagatag gcgcgggcga
     2701 cccgctcgtg ggtggagacc atgacgtcga ccgagtcgac gaagacctgg cggtcctggg
     2761 tctccggccg caggatggcc tccgtgaaga tcgcgtcggg gtgcatgaag atgcgtccga
     2821 ggaaggtcga cgcgaacttc tcggtgatcc ggtagccgag tcggctggcc gccacgacgc
     2881 gcccgtcatc gaggacgacg tcggcgaccg gctcgaggta gccggcctcg atgagcgccg
     2941 ccgccgaacg ctcggccggc gacatcctgg cgaagagctc ggggatgagc agactgacgt
     3001 cgtggtcgac gcggacctcg ggcccgacat agccggcgcc ggagatccag ccgtcatagc
     3061 ccgtcagggc gaaggagaga aaggcggcgt tgaggtcgta ggtcgtcggc agcgcgttga
     3121 agggcgcctt ggtcatcgcg ccctcggagc cggcgccggt cgtcgacggc gacttgccgg
     3181 tcatggagga gatgaactcc atgagcagtt cgggcagctc cagatagtgc agcggcccga
     3241 acgcgcacag ggcgggcacc ccgctctccg gtgggttgtt gcgccggccg gcggcgacga
     3301 tgtcgactcc ggtgaccatg ggctcgtcca tcgtcaggcc gcgggcgatc cggagggcgg
     3361 tgagcgcgcg tgccgtcccg gcggggtcgg ccatgtcggg ccgcacctgc aggtagcgcg
     3421 ggttcttcga cggcttgccg tcgacgatgc gcgggcgcgc cgaacagacc atataggtgg
     3481 cgtcgccggg cagcgtgccg gcggcgaagt cggtgatcag ctcgcgcatc ggctcggtga
     3541 actggctgac agcgatcgcg tcgtcggcca gctcggtcgc ggccgcgacg tcgagcggct
     3601 ggaagttgga caagaacgtt ccgggagagg cgatgtcggc ctcggcggtg gagtcgtagc
     3661 cgggagtgat cgcgtcgtcg ggccgctgga aaagcagccg ctcgcagttc tcgacatatt
     3721 tcaccgacgg ctcgcccgga ccgctggtag gcgcgacgac tgaggccgtg atgtcgtcct
     3781 cggtctgcac cttcaccgag ggcgcgaagt ccggccggag cgagaagagc cgataggagc
     3841 cgtccgactc gaacccgacg cgcagcatgt cgacgaggac ggcctgcccg tcgatccgca
     3901 ggcggttgcc ggtgcggccg ttgacttggc tgaccgagca gtggctacgc cagtccgcgc
     3961 cccactccga gcggtaggcc cgcttgatga catagaccag ttcgagcacg tgcgggggga
     4021 tggcccggat gaaggcgttg tgctcgtcgg agaactccgc cttgggcgtg aagagtttga
     4081 tgacgctgcc ggtgctgcgc tccgggccga ggaccgagcg gtggtccttg ccgttccact
     4141 cggggttggc gtagcgctcg ctgtagtcgc ggctgacgat ctgctcgacc gcgtccatgt
     4201 ccgcgtcgaa gtcggcgacg aacgcctgcc cgatcgtgat cgcgtcggcg atcgacttgg
     4261 agatctcgga cttgccgccg ccggagacgg tagacggctt gtggcagacc gtcgaggtcg
     4321 cggccgtgcc gatgatggtc cactgcaccg gttcccggcg cacctgcttc atgacgacgc
     4381 ggaagccgga ggtgtcgaga taggtggtgt ccccgcggaa ggggatggtc acggtgtcct
     4441 tctccccggc ccatgagacg gtgcgctcct ggagggagaa cttggctccc gtggggacca
     4501 ggacgatgtc ctcgtggtcg acgcagcggg cccacccggc ctcgtccggc gtccacgcgc
     4561 gcgggtcgcg cgcggtgacc tcggccacgg acggtgcgcc ttcggcatac gtgttgacga
     4621 actcctggcc ctcgtcccac gagggcagca cgatcgcgcc gccggagtgc tcttcctccg
     4681 acccgccgaa aaggttggcg gacatggaga tctgggtctt gacttccttc ttgcagtagc
     4741 cgaagtagtt gtcggcgatg atcgtcacca tgacgccgcg ctcgtcgcgg gcgcacacct
     4801 tgaaggcctg gccgtcgttg tagcgctccg ccgggtcggt ccagcacatg ccgtcgcgga
     4861 tctgccgctc ggtggcatcg tccttgtgcg ggaggccgag ggactgtttg gtggccgaaa
     4921 tcaggtgcgg cgcaaggaga accaggccgg tgtggccggt ccagcccagc gggtcgaggg
     4981 cggcgtcgtt ctcgggcagg taggggtcgc cggcgttgcc gaagattccc tcgacgaagt
     5041 ccaggttggc gaccatcgat ccgggcacga tgaagcgggt ctccatccgg cgctcatgcg
     5101 tatgcccggg cacgccgacc gtgaccagtg gccgcagcag cagggagacg aagcaggccg
     5161 ccttctcttc ctgggtggag gtgatgggca gttccagcgc cgcggccggg ggcgtgagcg
     5221 cgcggtcgag gagcgcggcg aacacctccc ggggcacggc cttcttgtcg tcggggatcg
     5281 gcaggccgcc ctcggcgatg tggaagacgc cggcggtggt gcggcggtcg ctgcgggggt
     5341 tgtgcagcac gccgttggcg aggcggtagc tcgtgatcag gccggaggag tactcgtcgg
     5401 agtcgaccgg cagcgagaga gtgcgcgcca ggccgggtag gtcgaggctc agggtgcgcc
     5461 ggggcaggtg cgggatcggt gtgacaccgt gcaggtatgc cgtcaggaac gcctcgatcc
     5521 gctcgtccac cggcgggagg cggtcaccga ggcggcggct cagctcgcgc tgccgcttca
     5581 ggatcggggc gaccaggtcg tcggcgacgg cctgacccgg ttccaccgcc tcgaccttgg
     5641 gttgccccag gaggtcgagc cgcacgttga cggcggcgag caggtctgtg gacgacgcgg
     5701 gctgggtgcc gttcatacct ccattgtgcg cctcatgaaa ccgcttacgc acccggttca
     5761 ccacgtggtc gttttcgggt cgccagatgg tcacccagcc gggcacgcgt gcgccggaag
     5821 gggtcagtgt gacggcgacc ggagcgcggg tgacctgtgt gcgggtgacc tcagtgcggg
     5881 tgtccggggt gggggctgcg gcgcgggcac aggacttcag tggccgcggc atatgacagc
     5941 agcgcggtgt cggcgtaggg gcggccggcg aacgtgaggc cgaccggcat gctgatgtcg
     6001 cgcatcacgc ccatcgcgat ggtgacggtc gggaggccga aatggcgcgg cacgagattg
     6061 ccgttggcga cccagacgcc gttgcgccag gcgagatcgg cggaggcggg atcgacgtcg
     6121 gcatccgccg ggccgacgtc ggcgaccgcg gggaagatca ccgcgtcgag ccgctcgctg
     6181 tcgagccacg catcgaggtc cagctcgcgg gtgcggtcca gacccgcgat cccctcggcg
     6241 acccatgtgt gctcacgcag cggaacgagc gggtgggcgc gggcgtagcc ggggaagagc
     6301 gcgatgtcgt cgccgaagtc gtgatagcga tccttcagcg cgcctggcgg gggcggaaag
     6361 atctgtgccc catcgatatc cgccaggcgg tgcaggccag ggtcaccgtt ggcgtggagg
     6421 aagtcgtccc acgaccaggc ggcgagatcc tggacctccc gctcgagata gaccgggctg
     6481 acgagaccgc gggtgcggat ggtggcagcg ccgggccggt cggcctcgta gttgctgacg
     6541 atcgggaagt cggtgaggac gacctcggct cccgcggcgc gcacgtcctc gaccagcgcc
     6601 cacagcaggt ccgtcggcac cagcggccag ttgccccgga tggagatcac cccgcgcgat
     6661 ggcgtatagg cgcacaggcc gttgttcgac gccggtccgc gaccggagga ccacgtctcc
     6721 tccccgaggc cgaaggcggc caaactcgcc gccgttgccg tgccggaacc gttggaggag
     6781 ccggaggcga aaggtgccgt gaggtatgcc gggttgtacg ggctctccgc gcggccgtag
     6841 agcccccgct gcatcccgcc gttggccatc ggcggcatat tcgtcaaccc caggaggatg
     6901 gcgccggcgc cgcggaggcg acccacggca tacgaatcgc gttgggccac aaggtgagcg
     6961 aacgcggggg agccgcccgc gacagtcagt cccgtcgcgg agtaggaatc cttggccgta
     7021 taggggatgc cgtcgagcgg gcccagcggc tcgccccggg tgcggcgccg gtcggactcc
     7081 tccgcctcgg cgcgggcgtt cgggtcgagg acgacgacgg agttcaggtg cggccccgcg
     7141 tggtcgtatg ccgcgatccg cgcgaggtag gcgtccacca gttccgcgct ggtcacctcg
     7201 ccccgtgcca gcgccgcggc gagctggacg atcccggcct ccaccacgtc atacgacatc
     7261 tctcgacgct atccccttga cacggaagcg gccagcgtgt gtacggtgtc aacattgcaa
     7321 gtggacagtg tccacactga tcgatggagg acgagatgag cgcgaccacg gacttgacca
     7381 cagacttgtc ggtgaccacg gctagcgttt cggccatgac cgagggaccg gagggcagcc
     7441 cggccgacgg ccccgacgcg cccggccata gcgagcacca cgccgcccgc ttgccgccgg
     7501 gcatcacccc cggcccgcag acattgcgcg ccttccgggc cgtgctcgcc aacacgctcg
     7561 tggccaatgt gacgaccagc tatttgtggt tcgccctgac gttctgggcc tatctcgaga
     7621 ccaagtcggt gctcgcgacc tcgatcctcg gcggctccta catgctgctg atcgccgtgg
     7681 gtggcatctt cttcggcacg ctcgtcgatc accaccgcaa gcacgccgtc atggtggcgt
     7741 ccagtgcgat cactctcgtc atgttcatgc ttgcgggggc gttgtacctg ctgttcccga
     7801 cgcagcgcct ggtcgactgg aacggacccg tcttctgggc gttcgccggc gtgatcctgc
     7861 tcggcggagt cgtcgagagc ctgcgcaata tcgccctgtc caccaccgtc accctgctgg
     7921 tccccgacgg ccagcgcgac aaggccaatg gacaggtcgg caccgtccag ggcatcgcct
     7981 tcatgatcac cagcgtcttc agcgggctgt cgatcgggct gctcggcatg ggctggaccc
     8041 tcggcctcgc catcggcctg accgcgctcg cgctgggaca cctgctcacc atcaccatcc
     8101 ccgagcaacg gcccccgcgt gacgagggcg cccggccccc ggccttcgac atccggggtg
     8161 cgatcggcgc cgtccgggcc gtcccgggcc tcggcgcgct gatcctcttc tcctgcttca
     8221 acaatctggt cggcggcgtc tacatggctc tcatggatcc ttatgggctg acgctgttca
     8281 gcgtcgagtc ctgggggatc gtgctcggcg tgacgagctt cggcttcgtc ctcggtggcc
     8341 tgtgggtggc ccggttcgga ctgggagaga agccacttcg ctcactcatg ctcgtcaacg
     8401 tcgtcgtcgc cctgatcggg atcttcttca ccgtccgcga ggtgtggtgg gtgtatgccg
     8461 gcggcatcct gctcttcatg tgcgtcatcc cgatcgccga ggcggccgag cagacgatcc
     8521 tgcagcgggt ggtgcccttc gccaagcagg gacgcgtctt cggcttcgcg cagagcgtcg
     8581 aggtggcagc gagtccgatc tcggccttcc tcatcggccc catcgcgcaa tacggtctca
     8641 tcccctatat gaagaccgcc gaggggcagg agcgtttcgg gtggctcgtc ggcgccggcc
     8701 aggcccgcgg catcgcgctg gtcttcatcg cctccggcct gctcatgctc gtcgccgtcg
     8761 ctgtggcgat gatctccccg ccctatcgtg tgctttcgcg ctcctaccag caggccgagc
     8821 cgccgcccgc gtcatgatgg gacggtgaag gctgacgagg cacgggatgc gggcaaggcc
     8881 gccggcgccg gtttgcgcac cgggaccggg ctgatccggg acatccacgg cgggatcagc
     8941 cacggggtgt atgccgtcgt cgacgccctc gccgggcctg cggcccggcc catccgggtc
     9001 gttcaggaca cgatcaccga gggcgtgtat gccgtgaccg gacgcggtct cgacgtgggc
     9061 agccggctcg tcggcgccgc cgtcgccgcc cgcaaggccc gttccggcgc cgaccacgcg
     9121 agcctgcacg acgggccggc ggctcaccac gtcatcgcga tcggcagtgg cctcatcgac
     9181 gattcccttg tgggacaggt cccgtcgctg cgccccacga tgcacgtgcg cgaacgcggc
     9241 acccgagtgg aactgacgcg cgagggcgtc ggcgcggcat accccgaagc gacggacgag
     9301 atcgtcgtct tcctgcacgg gctcgtggag accgaggcgg cgtggcggct gtcggccaag
     9361 acccgcccgc cgtatgccga ccggttgcgc gccgacctcg gtctgacccc ggtgatggtg
     9421 cgctacaaca cggggcttcg ggtgtcggag aacggtcgcc tcttcgccgg cctgctggcg
     9481 gatctcgtcg aggcctggcc ggtcccggtg cggcgcatcg cgctgatcgg gcactcgatg
     9541 ggcggcctga tcatccacag tgcgttcgcg caggccgagg acgacccggc gacacagtgg
     9601 cgtggcctgg tcaccgatac gatcacgctc gggacgcccc accatggctc accgctggcg
     9661 cgtggagcgt tcgcggccac ggcgtggctc ggacgctcac gccatacgcg gactgcagcg
     9721 caggtgatgc gtgcgcgcag cggctccgtg cgcgacctgg ctcacggcaa tatccttgcg
     9781 gcagactggg aaagcctcga cgtagaggag ttggtcgacg gccgcaccca tcccgctccg
     9841 gcaatgggca tcctgcaccg ggcggttggc gcggcatggg gcaaggcgca tccggacggc
     9901 gggcacgacc gactcagcga cttcctcgtg cccgtcacgt ccgccaccca cgcccacctg
     9961 gcaggggagg cacgccggtt caccgaggac gacatcgccg tcgtccacgg cacccaccat
    10021 ctgggcctgc tcaacagcga ggagacctac cagcacctgc tccgctggct gacccccgct
    10081 a
//
LOCUS       DAOPJG010000126        10078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7008_length_10078_cov_8.225781, whole genome shotgun sequence.
ACCESSION   DAOPJG010000126 DAOPJG010000000
VERSION     DAOPJG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7008_length_10078_cov_8.225781"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKU75_11415"
     CDS             <1..391
                     /locus_tag="PKU75_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPF81533.1"
                     /translation="TLMPAGIGSWSAADGFATSWDLDEVLEGDDVPDAVMMLRVQRER
                     MSGGFFPTPREYTVGYGLTRPRLKALLARNPRALVMHPGPMNRGLEISADAAESDASI
                     VLDQVSSGLAVRMAVLYHLLAGEEVAS"
     gene            388..1683
                     /locus_tag="PKU75_11420"
     CDS             388..1683
                     /locus_tag="PKU75_11420"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPF81534.1"
                     /translation="MRDLLITGAKIADGEPADVLIRDGAIAEIGAIESADGIERLDAD
                     GLVLLPGFVDLHTHLREPGREDAETIASGSAAAAAGGFTAVLAMANTTPVTDTAEAAE
                     LVYDRGRAIGLVDVFPVGAVTKGLAGEELAELGLMARSRARVRLFSDDGTCVQDARVM
                     RRALEYLKPFDGVISQHAQDVSLAGGSACCHEGEISGRLGLPGWPGAAESVIVARDVQ
                     LARHTGSRVHVAHVSTAESVEIIRWAKAQGIAVTAEATPHHLALGTKLVTGYDPVFKV
                     NPPLRPSEDQEALRAALADGTIDAVATDHAPHARHDKEHAFEAAAFGMLGLETAFAVV
                     HDLMVRSGRMPLADLVERMTVAPARIAGLAEHGQPIAVGGPGNLVLVDPERRVTVDAA
                     ASNSLSRNNPWHGMSFTGAVHATILRGRVTALDGKVHDQ"
     gene            1686..2828
                     /gene="carA"
                     /locus_tag="PKU75_11425"
     CDS             1686..2828
                     /gene="carA"
                     /locus_tag="PKU75_11425"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPF81535.1"
                     /translation="MTTSPVDTAVLVLEDGRRFTGRAFGARGTTVGEAVFNTGMTGYQ
                     ETLTDPSYHRQVVVMTAPHVGNTGWNDEDDESGRIWVSGFVVRDPALRPSNWRSSRSL
                     DDELAAQGVVGISGIDTRALTRHLRERGAMRVGIFSGPEASNGEREQLATVTGAPLMT
                     GAALAPEVSTSEPYVVAPVGEKRFTVAALDLGIKAMTPALMAQRGIEVHVLPATATFE
                     DIQAIEPDGVFFSNGPGDPATAEHEVGVLRQVLDARIPFFGICFGNQLLGRALGFGTY
                     KLRYGHRGINQPVMDVTTGKVEVTAHNHGFAVEAPLGEPVPAPAGPAYGKVRVSHVCL
                     NDDVVEGLECLDLPAFSVQYHPEAAAGPHDAAYLFDRFVSLLEGSR"
     gene            2831..6136
                     /gene="carB"
                     /locus_tag="PKU75_11430"
     CDS             2831..6136
                     /gene="carB"
                     /locus_tag="PKU75_11430"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPF81536.1"
                     /translation="MPRRTDISSVLVIGSGPIVIGQACEFDYSGTQACRVLRDEGIRV
                     ILINSNPATIMTDPEFADATYVEPITSEVIERIIEKERPDAILATLGGQTALNAAMTL
                     HEKGILDKYDCPLIGANVEAIQLGEDREKFKGVVERCGAESARSVICHTMDECLAAAT
                     ELNYPVVVRPSFTMGGLGSGFAYDEDDLRRIAGAGLQYSPVTEVLLEESILGWKEYEL
                     EVMRDHADNVVVVCSIENLDPMGVHTGDSITVAPALTLTDREYQRLRDIGIAVIREVG
                     VDTGGCNIQFAVNPDNGRIIVIEMNPRVSRSSALASKATGFPIAKIAAKMAIGYTLDE
                     VRNDITQETPASFEPALDYIVVKVPRFAFEKFPAADPTLTTTMKSVGEAMSLGRCFSE
                     ALQKALRSMEKAGSSFHWDGDPVSGEDLAELLAEIARPADGRIVGVQQALRAGASVEQ
                     VHEATKIDPWFLDQIHLLNEIAEEIRTAKHVAPGLLREAKRHGFSDKQIASLRGMSED
                     VVRGIRHALEVRPVYKTVDTCAAEFAARTPYYYSSYDEENEVEPRERPAVIILGSGPN
                     RIGQGVEFDYSCVHASFALRDAGYDTVMVNCNPETVSTDYDTSSRLYFEPLTLEDVLE
                     VIHAEEAAGPVAGVIVHLGGQTPLGLAQKLKDEGVTIVGTSPEAIHLAEDRGAFGRVL
                     AQAKLPAPKHGTAYSAQEAIAIAGEIGYPVLVRPSYVLGGRGMEIVYDDETLSEYVTR
                     AALASPEHPILVDRFLDDAIEIDVDALYDGEEMYIGGIMEHIEEAGVHSGDSACTLPP
                     ATLGKQELDRVRRSTLALAEGIGVRGLMNVQFALAHDVLYVLEANPRASRTVPFVAKA
                     TGVPLSKAAARVMLGASIADLRREGMLPADADGGSLPADAHMSVKEAVLPFKRFRTID
                     GTVVDSLLGPEMRSTGEVMGIDRDFGSAFAKSQLGATSGLPTSGRIFVSMANRDKRAM
                     IFPVMRLRDLGFTIVATTGTADVLRRNGIEAEVVKKVSERGPDEDGSIVTQILAGRID
                     MVVNTPSGQNAREDGYKIRQATTSMDKPIITTVQQLGAAVQGIESAQKDVLRVKSLQE
                     HARDLDLYGTRTA"
     gene            6133..7023
                     /locus_tag="PKU75_11435"
     CDS             6133..7023
                     /locus_tag="PKU75_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPF81537.1"
                     /translation="MTGRDMTAHDMTGRDAGVCQATARVAGNAPAAAYRHLTLDAPGI
                     AQLAQPGQFVALTVGGPTSALLLRRSFSIHRVDPAAGTVEIVVSDAGPGTAWITRLAD
                     GESVDIVGPLGRGFTLPDAPGAAVLIGGGYGSAPMFWLANVLRENGFAIHHVLGAASA
                     DRLFGAELADPADELIVTTDDGSQGERGWVSDPLPGLLERTGAKAVYACGPMGMLRAV
                     TEIATAHGARAEVAVEEAMACGIGVCMTCVLPVVGADGVTRMSRSCVDGPIFDATTVR
                     WDAVAGGRVAVPADCLGAPR"
     gene            7020..8048
                     /locus_tag="PKU75_11440"
     CDS             7020..8048
                     /locus_tag="PKU75_11440"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPF81538.1"
                     /translation="MSVDWSTSDVAGDDVPAVDLTTVDVTGVDLTADLGPNLQLPNPL
                     MTASGCAANGKELHRFGDVAQLGAFVTKSVKRDPQSGRGTPRMAETPSGMLNSIGLQG
                     PGIDAFVEHDLPWLKSVGARAIVSIAGASSGEFAEVAGRLVAAPDFDAVAAIEVNISC
                     PNVANRGLVFACDPASSTEVLRLVAPEVPSGIPLLAKLTPDVTDIVPIAGAALAGGAH
                     GLTMINTLLGIHIDTERMRPHLFGTTGGLSGPAIRPVAVRAIWQVHAAMRAGRIPAAP
                     IVGVGGVRTGQDALELTLAGASAIQVGTAIFNDPSAPWRVARELRDLLGQRGIPRYRD
                     LVGAAHQN"
     gene            8059..8889
                     /gene="pyrF"
                     /locus_tag="PKU75_11445"
     CDS             8059..8889
                     /gene="pyrF"
                     /locus_tag="PKU75_11445"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPF81539.1"
                     /translation="MATFGERLRASMDAHGALCVGIDPHPQLLQQWDLTVDADGLARF
                     TEICVEAFAGRVGAVKPQSAFFEEYGSAGIAVLENTIRAFRDAGTPLILDVKRGDIGS
                     TMAGYARAYLADGAPLASDAITVSPYLGFGSLTPALDQARESGRGLFVLALTSNPEGF
                     EVQHAVRGDRSVAAGVVAGVTEANAEASARGILGSIGLVVGATVGTAVQDLGIDLAAA
                     NAPILAPGFGAQGAGPEDARRVFGAAIPNVLASSSREVLGVGPRVADLRGAADSARLH
                     "
     gene            8974..9291
                     /gene="mihF"
                     /locus_tag="PKU75_11450"
     CDS             8974..9291
                     /gene="mihF"
                     /locus_tag="PKU75_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HPF81540.1"
                     /translation="MALPPLTPEQRAAALGKAAVARQERAAVKNRLKHASGSISEVIE
                     DGKTNDVIGKMRVSALLESMPGVGKVRAAAIMDELGISPTRRVRGLGANQAAALRERF
                     PAN"
     gene            9311..9880
                     /gene="gmk"
                     /locus_tag="PKU75_11455"
     CDS             9311..9880
                     /gene="gmk"
                     /locus_tag="PKU75_11455"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPF81541.1"
                     /translation="MSTGRLTVLAGPTAVGKGSVAAYIRENVPSVWLSVSVTTRAPRP
                     GEVDGVHYHFVDDATFERMRAEGDLLEWAVVHGRAKYGTPRRPVLQAIAQGRAPLLEI
                     DLQGARQVRETMPEALFVFLDPPSWDELVRRLVGRGTEGEDERERRLATARVEMAAAD
                     EFDVRIVNDDVERAARELVSLMSSPNPEM"
     gene            9891..>10078
                     /gene="rpoZ"
                     /locus_tag="PKU75_11460"
     CDS             9891..>10078
                     /gene="rpoZ"
                     /locus_tag="PKU75_11460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPF81542.1"
                     /translation="MSGTLPNPTGITSPPIDDLLTHADSKYALVIYSAKRARQINAYY
                     SQLQEGLLEYVGPLVDSQ"
BASE COUNT         1571 a         3686 c         3365 g         1456 t
ORIGIN      
        1 gaccctcatg ccggcgggca tcggatcctg gtccgccgca gacggattcg ccacgtcctg
       61 ggatctcgac gaggtcctgg aaggcgacga cgtgcccgac gcagtcatga tgctgcgggt
      121 ccagcgcgaa cggatgagcg gcggcttctt cccgaccccc cgcgaataca ccgtcggcta
      181 cggcctgacc cgtccgcgtc tgaaagccct gctggcccgc aacccccgcg ctctggtcat
      241 gcaccccggg ccgatgaacc ggggcctgga gatcagcgcc gacgccgccg agtccgacgc
      301 ctcgatcgtt ctcgaccagg tgtcgagcgg attggcggtg cggatggccg tgctctatca
      361 cctgctcgcc ggagaggagg tcgcgtcatg agggacctgc tcatcaccgg cgccaagatc
      421 gccgacggcg agcccgccga tgtcctcatc cgagatgggg cgatcgccga gatcggtgcc
      481 atcgaatcgg ccgacggcat cgagcgtctc gacgccgacg gactggtgct cctgcccggg
      541 ttcgtcgacc tgcacaccca cctgcgggag ccgggccggg aggacgccga gacgatcgcg
      601 tccggctcgg cggcggcggc cgcgggcggc ttcaccgcgg tgctcgcgat ggccaacacc
      661 actcccgtca ccgacaccgc cgaggccgca gaactggtgt atgaccgtgg ccgcgccatc
      721 gggcttgtcg acgtcttccc ggtcggagcg gtgacgaaag gccttgcggg agaagaactt
      781 gccgagctcg ggctgatggc acgctcccgc gcgcgcgtgc ggctgttctc cgacgacggc
      841 acgtgcgtgc aggacgcccg ggtcatgcgc cgcgcgctcg aatacctcaa gcccttcgac
      901 ggcgtcatct cccagcacgc ccaggacgtc tcgctcgccg gcggatcggc ctgctgccac
      961 gagggtgaga tctccggccg cctcggcctg cccggctggc ccggcgccgc tgagtccgtc
     1021 atcgtcgccc gcgacgtgca gctggcacgg cataccggtt cccgggtgca cgtcgcgcac
     1081 gtctccaccg ccgagtccgt cgagatcatc cgctgggcca aggcccaggg gatcgccgtc
     1141 accgccgagg cgaccccaca ccacttggcg ctcgggacca aactcgtcac cggttacgac
     1201 ccggtcttca aggtcaaccc gccgctgcgc ccgagcgagg accaggaagc cttgcgcgcc
     1261 gcgctcgccg acggcaccat cgacgcggtc gccaccgacc acgccccgca cgcccggcac
     1321 gacaaggaac acgccttcga ggcggccgcc ttcgggatgc tcgggctgga gacggcgttc
     1381 gcggtcgtcc acgacctgat ggtccgctcg ggccggatgc cactcgcgga cctggtcgag
     1441 cggatgacgg tcgccccggc gcgcatcgcc ggcctggccg aacacgggca gccgatcgcc
     1501 gtcggcgggc cgggcaacct cgtgctcgtc gaccccgagc gtcgggtcac cgtggatgcg
     1561 gcggcctcga attcgttgtc ccgcaataat ccctggcacg ggatgagctt caccggcgcg
     1621 gtgcacgcga ccatcctgcg cggccgggtc accgccctcg acgggaaggt ccacgaccag
     1681 tgagcatcac cacctcgccc gtcgacaccg cggttctcgt cctcgaggac gggcgacgtt
     1741 tcaccggccg ggccttcggc gcccggggca ccacggtcgg cgaggccgtc ttcaacaccg
     1801 ggatgaccgg ctatcaggag accctgaccg acccgagcta tcaccggcag gtcgtcgtca
     1861 tgacggcgcc gcacgtgggc aacaccgggt ggaacgacga ggacgacgag tcgggccgga
     1921 tctgggtgtc cggcttcgtc gtccgcgatc ccgcgttgcg accctcgaac tggcgctcct
     1981 cccgttccct cgacgacgag ttggccgccc agggcgtcgt cggcatcagc ggcatcgaca
     2041 cccgcgccct gacccgtcat ctgcgcgaac gcggcgccat gcgcgtggga atcttcagcg
     2101 ggccggaggc tagcaacggg gagcgcgagc aactcgcgac cgtcaccggg gccccgctga
     2161 tgacgggtgc cgctctggct cccgaggtca gcaccagcga gccgtatgtc gttgcgcccg
     2221 tgggggagaa gcggttcacc gtcgccgccc tcgacctcgg catcaaggcg atgaccccgg
     2281 ccctgatggc gcagcgcggc atcgaggtcc acgtcctgcc cgccaccgcc accttcgagg
     2341 acatccaggc catcgagccc gacggcgtct tcttctccaa cgggcccggc gacccggcta
     2401 cggccgagca cgaggtcggc gtgctgcgtc aggtgctcga cgcccgcatc ccgttcttcg
     2461 gcatctgctt cggcaaccag ctcctcggcc gcgctctcgg cttcggcacc tacaaactgc
     2521 gctacggcca ccgtggcatc aaccaacccg tcatggacgt cacgacgggc aaggtcgaag
     2581 tcaccgccca caaccacggc ttcgccgtcg aggcgccgct gggcgagccg gtgccggcgc
     2641 cggccggccc ggcatacggc aaagtccggg tctcgcacgt ctgcctcaac gacgacgtcg
     2701 tcgaaggcct ggaatgcctt gacctgccgg ccttttcggt gcagtaccac ccggaggccg
     2761 cggccggccc gcacgatgcg gcctaccttt tcgaccgctt cgtgagcctg ctggaaggga
     2821 gccggtagac atgccgcgcc gcaccgacat ctccagcgtc ctcgtcatcg gctccggccc
     2881 gatcgtcatc ggacaggcct gcgagttcga ctactccggc acccaagcct gccgcgttct
     2941 gcgcgacgaa ggcatccggg tcatcctgat caactccaac ccggcgacga tcatgaccga
     3001 cccggagttc gccgacgcga cctatgtcga gccgatcacg tccgaggtca tcgagaggat
     3061 catcgagaag gagcgccccg acgcgatcct cgcgaccctt ggcgggcaga cggcgctcaa
     3121 cgccgcgatg accctgcacg agaaggggat tctcgacaaa tacgactgcc cgctcatcgg
     3181 cgccaatgtc gaggcgatcc agctcggcga ggaccgggaa aagttcaagg gggtcgtgga
     3241 gcggtgcggc gccgagtccg cccgctcggt catctgccac acgatggacg aatgcctcgc
     3301 cgccgcaacg gaactcaact accccgtcgt cgtccgcccg tccttcacca tgggcgggct
     3361 cggcagcggt ttcgcctatg acgaggacga tctgcggcgc atcgccggcg cgggcctgca
     3421 gtattcccct gttaccgagg tgctcctcga ggagtccatc ctcgggtgga aggaatacga
     3481 actcgaggtc atgcgcgacc acgccgacaa tgtcgtcgtc gtctgctcca tcgagaacct
     3541 cgacccgatg ggcgtgcaca ccggcgactc catcaccgtc gcccccgccc tgaccctcac
     3601 cgaccgcgaa taccagcggc tgcgcgacat cggcatcgcc gtcatccgtg aggtcggcgt
     3661 cgacaccggc ggctgcaata tccagttcgc cgtcaacccc gacaacgggc gcatcatcgt
     3721 catcgagatg aacccgcgcg tatcccgttc cagcgcactg gcatccaagg caaccggctt
     3781 tcccatcgcc aagatcgccg ccaagatggc catcggctac accctcgacg aagtccgcaa
     3841 cgacatcacc caggagacgc cggccagctt cgagccggcc ctcgactaca tcgtggtcaa
     3901 ggtgccccgg ttcgcgttcg agaagttccc ggcggcagac ccgacgctga cgacgacgat
     3961 gaagtccgtg ggggaggcga tgtcgctcgg acgctgtttt tccgaggcac tgcagaaggc
     4021 gttgcggtcg atggagaagg ccgggtcgag cttccattgg gacggcgacc ccgtatccgg
     4081 tgaggacctg gcggaactgc tcgccgagat cgcccggccc gccgacggcc gcatcgtcgg
     4141 cgtgcagcag gcgctgcggg ccggcgcgag cgtcgaacag gtgcacgagg cgacgaagat
     4201 cgacccgtgg ttcctcgacc agattcacct cctcaacgag atcgccgagg agattcgcac
     4261 ggccaagcat gtcgcccccg gcctgctgcg cgaagcgaag cgtcatggct tctccgacaa
     4321 gcagatcgcg agcctgcgtg ggatgagcga ggacgtcgtc cgcggcatcc ggcacgccct
     4381 cgaggtgcgc ccggtctaca agacggtcga cacctgtgcc gccgagttcg cggcccgcac
     4441 gccctactac tactcgtcat acgacgagga gaacgaggtc gaaccgcggg agcgcccggc
     4501 ggtcatcatc ctgggctccg ggcccaaccg gatcggtcag ggcgtcgagt tcgactactc
     4561 ctgcgtgcac gccagtttcg ccctgcgcga cgccggctac gacacggtca tggtcaactg
     4621 caaccccgag accgtctcca ccgactacga cacctccagc cggctctact tcgagccgct
     4681 gaccctcgag gacgtgctcg aggtcatcca cgccgaggag gccgccggcc ccgtcgccgg
     4741 ggtcatcgtc cacctcggcg ggcagacccc cctcggcctg gcgcagaagc tgaaggacga
     4801 gggtgtgacg atcgtcggca cctcgcccga ggcgatccac ctcgccgagg accgtggcgc
     4861 cttcggtcgc gttctggcac aagcgaaatt gccggcgccg aagcacggca cggcatacag
     4921 cgcgcaggag gcgatcgcga tcgccgggga gatcggctat ccggtcctgg tgcgtccctc
     4981 ctatgtcctc gggggccgcg gcatggagat cgtctacgac gatgagacgc tgagcgagta
     5041 cgtcacccgt gccgctctcg cctcacccga gcacccgatc ctcgtcgacc gcttcctcga
     5101 cgacgcgatc gagatcgacg tcgacgccct gtatgacggg gaggagatgt acatcggcgg
     5161 gatcatggaa cacatcgagg aggccggcgt gcactcgggc gactccgcct gcaccctgcc
     5221 cccggcgacg ctgggcaaac aggagctgga ccgggtccgc cgctcgacct tggcgctcgc
     5281 cgaaggcatc ggcgtgcgcg ggctgatgaa cgtccagttc gccctcgcgc acgacgttct
     5341 ctatgtgctg gaagccaatc cgcgcgcctc gcgcaccgtg cccttcgtcg ccaaggcgac
     5401 cggagtgccc ttgtcgaagg ccgcggcccg ggtcatgctg ggtgcctcga tcgccgacct
     5461 gcgccgcgag gggatgctgc cggccgacgc cgacggcggg tcgctgccgg ccgatgcgca
     5521 catgtcggtc aaggaggcgg tgctgccgtt caagcgcttc cgcactatcg acggcacggt
     5581 cgtcgactcc ctcctcgggc cggagatgcg ctccacgggc gaggtgatgg gcatcgaccg
     5641 tgacttcggg tcggccttcg ccaagagcca gctcggtgcc acgagcggac tgccgacgag
     5701 cggccggatc ttcgtctcga tggccaaccg tgacaagcgg gcgatgatct tccccgtcat
     5761 gcggctcagg gacctcgggt tcacgatcgt cgccacgacg ggcaccgcgg atgtgttgcg
     5821 gcgcaacggg atcgaggcgg aggtcgtgaa gaaggtcagc gaacgcggcc ccgacgagga
     5881 cggcagcatc gtcacccaga tcctcgccgg ccgcatcgac atggtcgtca acacgccgtc
     5941 cgggcagaac gcccgggagg acggttacaa gatccggcag gcgacgacct cgatggacaa
     6001 gccgatcatc actaccgtgc agcagctcgg cgccgcggtg cagggcatcg agtcggcaca
     6061 gaaggacgtc ttgcgcgtga agtccctcca ggagcacgcg cgcgacctcg acctctacgg
     6121 cacgaggacc gcatgactgg gcgcgacatg acggcgcacg acatgactgg gcgcgacgcg
     6181 ggcgtctgcc aggcgaccgc gagggtggcg ggcaacgccc cggcggcggc ataccgccac
     6241 ctgacgctcg acgcacccgg gatcgcgcag ctcgcgcaac ccgggcagtt cgtcgccctc
     6301 accgtcggcg ggccgacctc cgccctgctg ctccggcgct ccttctccat ccaccgggtc
     6361 gacccggcgg ccggcacggt cgagatcgtc gtctcggacg ccggccccgg caccgcgtgg
     6421 atcacccggc tcgccgacgg tgagagcgtc gacatcgtcg gcccgctggg tcgagggttc
     6481 accctccccg atgcgcccgg agcggccgtg ctcatcggcg gcggctatgg ctcggcgccg
     6541 atgttctggc tggcgaatgt cttgcgggag aacggcttcg ccatccacca cgtgctcgga
     6601 gccgcgagcg cggaccggct cttcggggcc gagctggccg acccggccga cgagctgatc
     6661 gtcaccaccg acgacggctc gcagggggag cgggggtggg tcagcgaccc gctgccgggg
     6721 ctgctcgaac gcaccggcgc gaaggccgtc tatgcctgcg gcccgatggg tatgttgcgc
     6781 gcggtcaccg agatcgcgac tgcgcacggc gcgcgcgccg aggtcgcggt ggaggaggcc
     6841 atggcctgcg gcatcggggt ctgtatgacg tgtgtcctgc ccgtcgtcgg cgccgacggc
     6901 gtcacccgga tgagccgctc gtgcgttgac gggccgatct tcgacgccac cacggtgcgg
     6961 tgggacgccg tcgccggtgg ccgggtcgcc gtgcccgccg actgcctggg agcgccgcga
     7021 tgagcgtgga ttggagcact agcgacgtgg ccggcgacga cgtgccggcc gtcgacctga
     7081 ccaccgtcga cgtgaccggc gtcgacctga ccgcggatct gggcccgaac ctgcagctgc
     7141 ccaacccgct catgacggcc tccgggtgcg ccgccaacgg caaggaactg caccgcttcg
     7201 gcgacgtcgc ccagctcggc gccttcgtca ccaagtcggt caagcgtgac ccgcagtccg
     7261 gccgcggcac cccccggatg gcagagacgc cgagcggcat gctcaactcc atcggcctcc
     7321 aaggcccggg catcgatgcc ttcgtcgagc acgacctgcc gtggctgaaa tccgttgggg
     7381 cgcgggcgat cgtctccatc gccggcgcca gttccggcga gttcgccgag gtcgccggcc
     7441 ggctggtcgc cgcccccgac ttcgacgcgg tcgcggccat cgaggtcaat atctcctgcc
     7501 ccaatgtcgc caaccgcggc ctggtcttcg cctgcgaccc ggcgtcctcg accgaggtgc
     7561 tgcggctcgt cgcgcccgag gtcccgagcg gcatacccct gctcgccaag ctcaccccgg
     7621 acgtcaccga catcgtccct atcgccgggg cggcgctcgc cggcggcgcc cacggtctga
     7681 cgatgatcaa caccctgctc ggcatccaca tcgacaccga gcggatgcgt ccgcacctgt
     7741 tcggcaccac cggcgggctg tccggcccgg cgatccgtcc cgtcgcagtc cgtgcgatct
     7801 ggcaggtgca cgccgccatg cgcgccggcc gcatcccggc cgcgccgatc gtcggggtcg
     7861 ggggagtgcg cacggggcag gacgccctgg aactcaccct cgccggcgcc agcgccatcc
     7921 aggtcggcac agcgatcttc aacgacccca gcgcaccctg gcgcgtcgcc cgcgagttgc
     7981 gggacctgct ggggcagcgc ggcatacccc gctatcgcga cctcgtcggc gcggcacacc
     8041 agaactagag gagatcccat ggccaccttc ggcgagcgcc tgcgcgccag catggacgcg
     8101 cacggtgccc tctgcgtcgg catcgacccc cacccccaac tgctgcagca gtgggacctg
     8161 accgtcgacg ccgacgggtt ggcgcggttc accgagatct gcgtcgaggc gttcgccggc
     8221 cgcgtgggcg cggtcaaacc gcagtcggcg ttcttcgagg aatacggctc ggcgggaatc
     8281 gcggtgctgg agaacacgat ccgggcgttc cgcgacgccg ggaccccgct gatcctcgat
     8341 gtcaagcggg gcgatatcgg ctcgacgatg gccggctacg cccgcgccta cctcgccgac
     8401 ggggcaccgc tcgccagcga cgcgatcacc gtcagcccct acctcgggtt cggttcgctg
     8461 acgcccgccc tcgaccaggc ccgcgagagc ggccgcggcc tgttcgtgct cgccctgacc
     8521 tccaaccccg agggcttcga agtgcagcac gccgtccgcg gcgaccgcag cgtcgccgcc
     8581 ggcgtggtcg ccggggtgac cgaggcgaac gcggaggcga gcgcccgcgg catactcggc
     8641 agcatcggcc tggtcgtcgg cgcgaccgtc ggcacggcgg tccaggacct cgggatcgac
     8701 ctcgccgccg ccaacgcacc catcctcgcc cccggcttcg gcgcccaggg cgccgggccg
     8761 gaggatgcac gacgggtctt cggcgcagcc atacccaacg tcctggccag ctccagccgg
     8821 gaggttctcg gcgtcggtcc ccgcgtggcg gacctgaggg gagcagcgga ttcggcgcga
     8881 ctccactaac atagggtgat cacggcccgg ttccgggccg atcagtgcaa gggccccctt
     8941 cccgggggcc cttcccgatg agaggagttg agcgtggcac tccccccgct cacacccgaa
     9001 cagcgcgctg ccgcactcgg gaaggccgcc gtcgcccgcc aggagcgtgc cgcggtcaag
     9061 aaccgcctca agcacgcgag cggctcgatc tccgaggtga tcgaggatgg caagaccaat
     9121 gacgtgatcg gcaagatgcg cgtctccgcc ctgctcgaat ccatgcccgg ggtgggcaag
     9181 gtgcgcgctg ccgccatcat ggacgagctc ggcatcagcc cgacccgtcg ggtccgcggc
     9241 ctgggggcca accaggcggc cgcgttgcgc gagcgctttc cggccaactg accggaaggt
     9301 cactgagcgg atgagcaccg ggcgcctgac cgtgctggcc ggcccgacgg ccgtcggcaa
     9361 gggcagcgtc gccgcctaca tccgcgaaaa cgtcccctcc gtctggctct cggtgtccgt
     9421 caccacgcgg gcgccgaggc cgggcgaggt ggacggcgtg cattaccact tcgtcgacga
     9481 cgccaccttc gaacggatgc gcgccgaggg ggacctgctc gaatgggccg tcgtgcacgg
     9541 gcgggccaag tacggcaccc cacgccggcc ggtgctgcag gccatcgctc aggggcgcgc
     9601 acccctgctc gagatcgacc tgcagggcgc gcgccaggtc cgcgagacga tgccggaggc
     9661 gctcttcgtc ttcctcgacc cgccgagctg ggacgaactg gtgcgccgcc tcgtcgggcg
     9721 cggcaccgag ggcgaagatg aacgcgagcg gcgcctggca accgcgcggg tcgagatggc
     9781 ggccgccgac gagttcgacg tccgcatcgt caacgacgat gtcgagcgtg ctgctcgtga
     9841 actcgtatca ttgatgagtt cacccaatcc cgagatgtga cggtagaccc ttgtctggaa
     9901 cccttcccaa ccccacgggc atcaccagcc cgcccatcga cgacctgctg acgcacgccg
     9961 acagcaagta cgccctggtc atctattcgg ccaagcgcgc ccgtcagatc aatgcctact
    10021 actcccagct gcaggaaggc ctgctggagt atgtcggccc gctcgtcgac tcgcagat
//
LOCUS       DAOPJG010000127        10017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7062_length_10017_cov_8.241919, whole genome shotgun sequence.
ACCESSION   DAOPJG010000127 DAOPJG010000000
VERSION     DAOPJG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7062_length_10017_cov_8.241919"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1570)
                     /locus_tag="PKU75_11465"
     CDS             complement(140..1570)
                     /locus_tag="PKU75_11465"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPF81543.1"
                     /translation="MSRFASRPKRFAAFTSSLALAGAVLVGCGVAADDTSTTAAGSAV
                     TVSSVASTTSDSDTAVTASSVASTTSDSDTAVTASSVASTTSDSDTAGSGDSASTTSA
                     STSTTSATSITSTTSTDTHYDDDDLTWEASEEIAVTLADGASSGAGVTVDGDTVTITS
                     GGVYRISGTLSDGQLVVAAPDDETVVLILDGVSITNADGTAVAITSADEVTVYLEDGT
                     TNVLADGSNYTVADDATPVAALASASDLTIAGSGTLDVTGNAGDGISTKDGLVIASGT
                     VTVTAADDGIRGKDYVYVLDGTLSVTSGGDAVKSDNAEEEDRGWVRIDGGTLSIEAGD
                     DGVHSERALDITGGTVTVTQSVEGIESQTITISGGTVEVTASDDGINATAINGVTGGT
                     ESDDGSLLTISGGTVTVNAGQDGIDSNGSVALTGGTITITSASGGGTGAVDANGQVTL
                     TDATVTANGTQVTSADQLAMSHGGGR"
     gene            complement(1730..7081)
                     /locus_tag="PKU75_11470"
     CDS             complement(1730..7081)
                     /locus_tag="PKU75_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPF81544.1"
                     /translation="MGATIRLAVIDHGEPAVRLVAAIGNLNRDPGTRITSVVVAPDTT
                     APWFAREGDEFLSVPAAAGAEGLVAALQTAGIDAMWPSEVSAYGSLLEVVRSAEDAGM
                     TVLGPNAGAIERLTDPGNLATLAEQHGIPVDADGPAERLLEVDTLTDAAGTTWALPPR
                     EVTLQQDGELLIAESPISAIAEDVLEDVTAAARTLAGACAATCGATGSAIVTFAVPFD
                     GSAYAVRSLAVGGRPEHAAIEETTGASFVGLQWRIETGGSLDAAPPEGDGYTIEARIL
                     ARDPEGGHPTGGRVELFSLPVGTGVRVDASLREGDLVDAERDPVLATVTAWGLDRAQA
                     LSRLRAALERTAIALSDGLTNRSVLLAVLGSDDVAAGPVTAAWLPETLGGGALEPPPD
                     PMALAAAAVEFYEADFALVQDSFRASASRGRPAHPEQVGAQMQLTYRGQPHRYRVDKI
                     AADRYRIRDGAAVDVTVDRLSDHERRMVVAGRKVRVVVQPQEEAVHLEIDGVAHTVRR
                     DDGVVVRTGWPALVGSLLVSVGDDVTAGQPIAILESMKMVTTVTAPFSGHVTSVAVLP
                     NAQVEKGDPLLRIRTSASADEDTSNADVSLATLAETPSGAAPVSESARVFGGLTNYLL
                     GYDLDPAAVKVLIAAYRKLAAGEDSSELREREDAFLDLYAEIGSLYRPRVEADSGADP
                     DEEDPDNTQEFFIAYLQWLDPDRAGLPASYRVRLQQALARYGVDSLADREDLRRTTLW
                     MFRSFSRVPEMAPVVVAILERRMDKPDAEPTADWQDRLERLVAATEGRQPEVTDLARD
                     VHFQAFEQPAIERINDVTAAEMATHMGVLADDPHVADRSDRINRLVWSPHPMRSLLLD
                     SWRDSLASPADADQTQAFRAAILETYLRRFYRIRDLRGLDIDIVDGFEVAFAHYGHNG
                     MDLHVVAGYLPLEDLPRASRAIATHLSTKAFQREAIVDLVLWREGEWDDIEALAAHTA
                     DILAQCDFGRVLHRLDLTITTMGGHRASRLRTQHLTYRQGEDGSFVEEQIYRNLHPML
                     GKRLDLWRLSNFTLTRLPSPEDVYLFDGVAKDNPKDHRLFALAEVRDLTPVRNELSGE
                     VTYPLLGRMGLAALASMRAALATYPVRERPVANRLVLSIRPTWTIPATDWYPLARAYE
                     GLARGAGLEKIVLHVTVPETDPVGKRVLRDRILVLDGLGAGGLSIRMDEPGPNPVRPL
                     TKYAQKVLTANRFKSPYPYEILRMLTPNPGDASPFPSGRYVELDLDEDGETLIEVDRE
                     PGNNSAHVVVALITNYTDTQPEGMTRIALLSDPTQGLGNLAEPECRRINSVLAYALEH
                     KIPVEWYAVSSGALIAMDSGTENMDWIALTLRRLIEFTQAGGEVNIIVTGINVGGQPY
                     WNAEATMLMHTKGILIMTPASAMVLTGKQALDFSGAVSADDNSGIGGFDRIMGPNGQA
                     QYWAPSFPEACQLLLDHYSFTYVVPGERFPRRWKTADPVDRDVRTAPHAKIAGSDFTT
                     VGDVFGATTNPERKLPFDMRSVMRSVADADCEPLERWRAWQDSDTSIVWDATVGGIPV
                     CMLGLESRTVPRRGFVPSYGPPAWTSGTLFPQSSRKTARAVNAASGNRPLVVLANLSG
                     FDGSPESMRNWQLEYGAEIGRAVTNFDGPIVFVVVSRYHGGAFVVFSKALTESMEIAA
                     VEGSFASVIGGAPAAATVFAREVKVRTEGDERVKTARAEAASASGAEASAARAALARI
                     SAEVRSAKLREVADEFDGIHTIDRARRVGSVDRIIKAETLRPYVIDALERGMANFTG"
     gene            complement(7120..>10017)
                     /locus_tag="PKU75_11475"
     CDS             complement(7120..>10017)
                     /locus_tag="PKU75_11475"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF81545.1"
                     /translation="AAAPAAPAAGSDAVLAKVTEIVAEMTGYPADLLDPELDLEADLG
                     VDTVKQAEVFAAVRGNWDLERDENLKLRDFPTMNHVAGWVRDKLGMAAPSAAAAGAPA
                     AAGETTPAAAAPAAPEHPHGKLIKGDLRAIDALPRRIPVPALRPSAEQSVATGVELKG
                     ARVVVRGESSGVADALVKRLEKAGATVLYGPAENSDDEFAGRLAEWMAEGPIAGVYWL
                     PALDDEGDVTAYELNEWQGALQRRVKGLYATMRTLWETSPFLVVATRLGGFHGYDAGG
                     ATSVLGGAVTGFAKSYKKERPDALVKAVDLPASRKTAAIADLLIEETLRDPGCVEVGR
                     AADGLRYGVAFVERAFTPLGPDGSPHGEGGMTLGKDSVFVITGAAGSIVSAITADLAK
                     ASGGTFHLLDLTPSPDPDDEDINTFRDDKAALKARIATRLKEQGKKATPVLIDREIAR
                     IERLEAALTAIEAVTDAGGKAFYYPVDLTDAGNVAEVMEQVRERSGSIDVLLHAAGLE
                     ISRNLPEKEPREFNLVFDVKTTGWFNVWRSAKDMEVGAVVVFSSVAGRFGNQGQTDYS
                     SANDLLCKVVSNFRRTRPQTRGIATDWTAWGGIGMATRGSIPKIMEMAGVQMLPPEAG
                     VAWVRRELESSAYSGEVIVAGVLGAMAAEYHESGGLDRSAVGAGTGPMVGEVSSSVHT
                     GVTSKTTLTPAEQGFLTDHRIDGTPVLPGVMGMESFAEVASLIAPQGYRVAAVEDVSF
                     AAPVKFFRDEPRELTISAVITPAPDGDDLIAHCALTASRELPGQSAPVTTTHFTGTVV
                     LTKSEPEAQSVEGSTEAPEQTVGSDVVYTFYFHGPAYQVVSEAWKDNGGSVARFNTGV
                     PDNHVPADAPLITAPRLVELSFQTAGLWEAGTQGRLALPMRVASTRVLKDPASVEGDL
                     FARATPTADGFDVVVTDAAGDVVVVLDGYATVPLPGDLSEDVASALGATFA"
BASE COUNT         1371 a         3401 c         3678 g         1567 t
ORIGIN      
        1 ctggccccgc ttcagtcgaa cctggccccg cttcagtcga acctggcccc gcttcagtcg
       61 aacctggccc cgcttcgctc gaacctggcc ccgctcgacg ccggccccgt cgggggcgcg
      121 gcgtcgcagg tgggccggct cagcggccgc cgccgtggct catggccagc tggtcggcgg
      181 aggtgacctg ggtgccgttg gcggtgaccg tggcgtcggt gagggtgacc tggccgttgg
      241 cgtccacggc gccggtgccg ccgccggagg cgctggtgat cgtgatcgtg ccgccggtca
      301 gggcgaccga gccgttggag tcgataccgt cctggcccgc gttcacggtc acggtgccgc
      361 cgctgatcgt cagcagggag ccgtcgtcgg actcggtgcc gccggtgacg ccgttgatcg
      421 cggtggcgtt gatgccgtcg tcggaggcgg tcacctccac cgtgccgccg gagatggtga
      481 tggtctggga ctcgatgccc tccacggact gcgtcaccgt gacggtgccg ccggtgatgt
      541 cgagggcccg ctcggagtgc accccgtcgt cgccggcctc gatcgagagc gtgcccccgt
      601 cgatgcgcac ccagccacgg tcctcctcct ccgcgttgtc ggacttgacg gcgtcgccgc
      661 cggacgtgac gctcagggtg ccgtcgagga cgtagacgta gtccttgccg cggatgccgt
      721 cgtcggcagc tgtgacggtg accgtgcccg aggcgatgac caggccgtcc ttggtgctga
      781 tgccgtcgcc ggcgttgccg gtgacgtcga gggtgccgga gccggcgatc gtcaggtcgg
      841 aggccgaggc gagggcggcg acgggtgtgg cgtcgtcggc gacggtgtag ttcgagccgt
      901 cggcgaggac attggtggtg ccgtcctcga ggtagaccgt gacctcgtcc gcactggtga
      961 tcgctacggc ggtgccgtcg gcgttggtga tggacacgcc gtcgaggatc agcacgacgg
     1021 tctcgtcgtc aggggcggcg acgaccagct gaccgtcgct cagcgtgccg gagatccggt
     1081 agaccccgcc gctcgtgatc gtcacggtgt cgccgtcgac ggtgacaccg gcaccgctcg
     1141 aggcaccgtc ggcgagggtg acggcgatct cctccgaggc ctcccaggtc aggtcgtcat
     1201 cgtcgtagtg ggtgtcggtg gaggtggtgg aggtgatgga ggtagcggag gtggtggagg
     1261 tgctggcgga ggtggtcgac gccgaatcgc cggacccggc cgtgtcgctg tccgacgtgg
     1321 tcgacgccac ggagctggcg gtgacggccg tgtcgctgtc cgacgtggtc gacgccacgg
     1381 agctggcggt gacggccgtg tcgctgtccg acgtggtcga cgccacggag ctgacggtga
     1441 cggccgaacc ggccgccgtg gtcgaggtgt cgtcggcggc gacgccgcag ccgaccagca
     1501 ccgcgcccgc cagggcgagg ctggaggtga aggcggcgaa gcgcttgggg cgagaagcaa
     1561 agcgggacac aggaatcctc tttcgtcgtg gggggcggta tgccgtaccc ctagagattc
     1621 ccgtgccccg ctttcgcgct cttgtggtct cgctgtgccc tttctgtgag cctcagccgg
     1681 tggtttgcgg ctcagccggt ggtttgcggc tcagccggtg atgtgcggct tagccggtga
     1741 agttggccat gccgcgttcg agggcgtcga tcacgtacgg ccgcagtgtc tcggccttga
     1801 tgatcctgtc gacggacccg acccgccgtg cccggtcgat ggtgtggatg ccgtcgaact
     1861 cgtccgcgac ctcgcgcagt ttcgccgacc gcacttccgc gctgatccgg gccaacgcgg
     1921 cgcgggcggc ggatgcctcc gcgccggacg cgctcgcggc ctcggcccgg gcggtcttga
     1981 cccgctcgtc accctcggtg cgcaccttga cctcacgcgc gaaaaccgtt gcggcagcgg
     2041 gcgcgccacc gatgacggag gcgaaggagc cctccaccgc cgcgatctcc atcgactcgg
     2101 tcagcgcctt cgagaagacg acgaaggcgc caccgtgata gcgggagacg acgacgaaga
     2161 cgatcgggcc gtcgaagttc gtcaccgccc gcccgatctc ggcgccatac tccagctgcc
     2221 agttgcgcat cgactccggc gatccgtcga agccggacag gttcgccagg acgaccagcg
     2281 gccggttgcc gctcgccgcg ttgacggctc gcgcggtctt gcgcgacgac tgcgggaaga
     2341 gcgttcccga ggtccacgcc ggcggcccgt aggacgggac gaacccgcgc cgcggcaccg
     2401 tgcgcgactc cagtccgagc atgcagaccg ggatgccgcc gaccgtcgcg tcccacacga
     2461 tcgaggtgtc ggagtcctgc catgcgcgcc accgctccaa cggttcgcag tcggcgtcgg
     2521 ccaccgaccg catcaccgag cgcatgtcga acgggagctt gcgttcgggg ttggtcgttg
     2581 ccccgaagac gtccccgacc gtggtgaagt ccgagccggc gatcttggcg tgcggcgccg
     2641 tgcggacatc gcgatccacc gggtccgccg tcttccaccg gcgcgggaag cgttctccgg
     2701 gaacgacata cgtgaagctg tagtggtcga gcagcagctg gcaggcctcc gggaacgagg
     2761 gcgcccagta ctgcgcctga ccgttggggc ccatgatccg gtcgaagccg ccgatgccgg
     2821 agttgtcgtc ggcggagacg gcgccggaga agtccagggc ctgcttgccc gtcagcacca
     2881 tcgcgctcgc cggcgtcatg atcaggatgc ccttggtgtg catgagcatc gtcgcctcgg
     2941 cgttccaata gggctgcccg ccgacgttga tcccggtgac gatgatgttg acctcgccgc
     3001 cggcctgtgt gaactcgatg agccggcgca gggtcagggc gatccagtcc atgttctcgg
     3061 tgccggagtc catggcgatg agcgctccgg aggagacggc ataccactcc accgggatct
     3121 tgtgctccaa cgcgtaggcg aggaccgagt tgatccggcg gcactccggc tccgcgaggt
     3181 ttccgagccc ctgggtgggg tcggacagca gggcgatgcg cgtcataccc tccggctgcg
     3241 tgtccgtgta gttggtgatc agggcgacca cgacgtgggc gctgttgttg cccggctcgc
     3301 ggtccacctc gatcagcgtc tcgccgtcct cgtcgaggtc gagttcgaca taccggcccg
     3361 acgggaaggg cgaggcgtcc cccgggttgg gggtgagcat ccgcaggatc tcgtaggggt
     3421 agggcgactt gaaccggttg gccgtcaaca ccttctgggc gtacttcgtc agcggccgca
     3481 ccgggttggg gcccggctcg tccatgcgga tcgacaggcc accggcgccg agtccgtcga
     3541 gcacgaggat gcgatcgcgc aacacccgct tcccgaccgg gtcggtctcc gggaccgtca
     3601 cgtgcaggac gatcttctcc agaccggcgc cccgggcgag cccctcatag gcgcgcgcca
     3661 gcggatacca gtcggtcgcg gggatggtcc acgtcggccg gatcgagagc acgagccggt
     3721 tggcgaccgg gcgctcgcgc accgggtagg tcgccagcgc ggcccgcatc gacgcgagcg
     3781 cggccaggcc catccgcccg aggaggggat aggtgacctc gcccgagagt tcgttgcgca
     3841 cgggagtcag gtcgcgcacc tcggccaggg cgaagagccg gtggtccttg gggttgtcct
     3901 tggcgacgcc gtcgaagagg tagacgtcct cgggggaggg caaccgcgtc agggtgaagt
     3961 tcgacagccg ccacaggtcg agacgcttgc cgagcatcgg gtgcaggttg cgatagatct
     4021 gctcctcgac gaaggacccg tcctcgccct gccggtaggt caggtgctgg gtgcgcagcc
     4081 ggctggcccg gtgcccgccc atcgtcgtga tcgtcaggtc gaggcggtgc agcacacggc
     4141 cgaagtcgca ctgggcgagg atgtcagccg tatgggcagc gagcgcctcg atgtcgtccc
     4201 actcaccttc gcgccacagc acgagatcga cgatcgcttc gcgctggaaa gccttggtgc
     4261 tcaggtgtgt tgcgatggcc cgcgaggccc ggggcaggtc ctcgagcggc aggtagcccg
     4321 cgacgacgtg cagatccatg ccgttgtggc cgtagtgggc gaaggccacc tcgaagccgt
     4381 cgacgatgtc gatgtcgagg ccgcgcaggt cgcgaatgcg atagaagcgc cgcaggtagg
     4441 tctcgaggat ggcggcgcgg aaggcctggg tctggtccgc gtcggccggg gaggcgaggc
     4501 tgtcgcgcca ggagtcgagc aacagcgagc gcatcggatg cggtgaccag acgagccggt
     4561 tgatccggtc ggagcggtcc gccacgtgcg ggtcgtccgc caggactccc atgtgggtcg
     4621 ccatctcggc agcggtgaca tcgttgatcc gctcgatggc cggctgctcg aaggcctgga
     4681 aatgcacgtc gcgggcgagg tcggtgacct cgggctggcg gccctcggtg gccgcgacga
     4741 gtcgctcgag ccggtcctgc cagtcggcgg tgggctcggc atccggcttg tccatgcggc
     4801 gctcgaggat cgcgacaacg accggtgcca tctcgggcac ccggctgaag gagcggaaca
     4861 tccacaaggt ggtgcgacgc aagtcctcgc ggtccgccag cgagtccacg ccataccgcg
     4921 cgagcgcctg ctgcagccgc acccggtagc tggcgggcag tccggcccgg tccgggtcga
     4981 gccactgcag gtaggcgatg aagaactcct gggtgttgtc cgggtcctcc tcgtcggggt
     5041 cggcgccgga gtcggcttcc acgcgcgggc gatagagcga gccgatctcg gcgtagaggt
     5101 cgaggaaggc gtcctcccgt tcgcgcagtt cgctgctgtc ctcgccggcg gcgagtttgc
     5161 ggtatgccgc gatgagcacc ttgaccgccg ccgggtccag gtcgtaaccc agcagatagt
     5221 tggtcaggcc gccgaagact cgggcgctct cactgaccgg cgctgcgccg gaaggggttt
     5281 cggcgagcgt ggcgagactg acgtcggcat tcgaggtgtc ctcgtccgcg ctcgcgctgg
     5341 tgcggatccg cagcagcggg tcgcccttct cgacctgggc gttgggcagc acggcgaccg
     5401 aggtcacatg tcccgagaag ggcgcggtga ccgtggtgac catcttcatc gactcgagga
     5461 tcgcgatcgg ctggcccgcc gtgacgtcgt cgccgacgga gacgagcaac gatccgacga
     5521 gggccggcca accggtgcgc acgacgacgc cgtcgtcgcg gcgcacggtg tgggcgacgc
     5581 cgtcgatctc gagatggacg gcctcctcct gcggctgcac gacgacccgg accttgcggc
     5641 cggcgacgac cattcggcgt tcgtggtcgg agagccggtc gacggtgacg tcgaccgccg
     5701 cgccgtcgcg aatgcggtag cggtcggcgg cgatcttgtc gacccgatag cggtgcggct
     5761 ggccgcgata ggtcagctgc atctgggcgc cgacctgctc cgggtgcgcc ggacgcccgc
     5821 gcgaggcgct ggcgcggaag ctgtcctgca ccagggcgaa atcggcctcg tagaactcga
     5881 cggcggccgc cgccagggcc atcgggtcgg gcggcggctc cagggcgccg ccgcccaggg
     5941 tctccggcag ccaggccgcc gtgacgggcc cggcggccac gtcgtcgctg ccgagcacgg
     6001 ccagcaggac cgaccggttc gtcagtccgt cggacaaggc gatcgccgtc cgttccaaag
     6061 ctgcccgcag gcggctcagc gcctgggcgc ggtccagacc ccacgccgtg acggtggcga
     6121 gcacgggatc gcgctcggcg tcgacgaggt cgccctcgcg cagactggcg tcgacccgca
     6181 cacccgtgcc gacgggcagt gagaacaact cgacgcggcc gccggtcggg tgaccgccct
     6241 ccgggtcccg cgcgagaatg cgcgcctcga tcgtgtagcc gtcgccctcg ggcggtgcgg
     6301 cgtccaacga gccgccggtc tcgatccgcc attgcagccc gacgaaactc gcaccggtcg
     6361 tctcttcgat cgccgcgtgt tcgggccgcc cgcccacggc gagcgagcgc acggcatacg
     6421 cactgccgtc gaagggcacg gcgaaggtga cgatcgcgga gcccgttgcg ccgcacgtcg
     6481 cggcgcacgc gcccgcgagg gtgcgtgccg ccgccgtgac atcctcgagg acgtcctcgg
     6541 cgatggcgct gatcggcgac tccgcaatga gcaactcgcc gtcctgctgg agggtgacct
     6601 cccgcggcgg cagcgcccac gtcgtgccgg cggcgtcggt gagggtgtcg acctcgagga
     6661 gccgctcggc gggcccgtcc gcgtccaccg gtatgccgtg ttgctcggcg agcgtggcca
     6721 ggttgcccgg gtcggtgagt cgttcgatcg cgccggcgtt ggggccgagg accgtcatgc
     6781 ccgcgtcctc cgccgaacgc accacctcga gcagcgaccc gtaggcgctg acctcgctcg
     6841 gccacatggc gtcgatcccc gccgtctgga gggccgcgac gagcccctcg gcgccggccg
     6901 cagccggcac cgagaggaac tcgtcgccct cgcgggcgaa ccagggcgcg gtcgtgtccg
     6961 gtgcgacgac cacactcgtg atccgggtgc cggggtcccg gttgagattg ccgatagccg
     7021 ccaccaggcg cacggcgggt tcgccgtgat cgatcacggc cagccgaatt gtcgctccca
     7081 cgggtaaagc tcccttgatc tggtcgagag tggatggggt caggcgaacg tcgcgccgag
     7141 cgccgatgcg acgtcctcgc tgaggtcacc cggaaggggt acggtcgcgt agccgtcgag
     7201 gacgacgacg acatcgccgg ccgcgtcggt gacgacgacg tcgaagccgt cggcagtcgg
     7261 tgtggcgcgg gcgaacaggt caccctcgac gctcgccggg tccttgagca cccgggtcga
     7321 ggcgacgcgc atgggcagcg cgagccgtcc ctgggtgccg gcctcccaca atccggccgt
     7381 ctggaagctc agctcgacca ggcggggagc cgtgatcagc ggagcgtccg ccgggacgtg
     7441 gttgtccggg acgccggtgt tgaaccgcgc gaccgagccg ccgttgtcct tccacgcctc
     7501 ggagaccacc tgataggcgg gcccgtggaa gtagaaggtg taaacgacat cggagccgac
     7561 ggtctgctcg ggtgcctcgg tgctgccctc gacgctctgc gcctccggct cgctcttggt
     7621 cagcacgacc gtgcccgtga agtgggtggt cgtcaccggt gcactctggc cgggcagctc
     7681 gcggctggcg gtcagggcgc agtgcgcgat gaggtcgtca ccgtcgggag cgggggtgat
     7741 gaccgccgag atcgtcagct ctcgcggctc gtcgcggaag aacttcaccg gtgccgcgaa
     7801 cgagacgtcc tcgaccgccg cgacgcgata accttgcggc gcaatgagac tcgccacttc
     7861 ggcgaaggac tccatgccca tcacgcccgg cagcaccggg gtgccgtcga tgcggtgatc
     7921 ggtcaggaag ccctgctccg ccggggtcag cgtggtcttc gacgtcacgc ccgtgtggac
     7981 gctgctcgac acctcgccga ccatcgggcc ggtcccggcg ccgacggcgc tgcggtcgag
     8041 gccgccgctc tcgtggtact cggcggccat ggcgccgagc accccggcga cgatgacctc
     8101 gccgctgtat gccgaggact ccagttcccg acgcacccag gccacgcccg cctccggcgg
     8161 cagcatctgc accccggcca tctccatgat cttggggatg gatccgcggg tcgccatgcc
     8221 gatcccgccc cacgccgtcc agtccgtggc gatgccgcgg gtctggggcc gcgtgcggcg
     8281 gaagttggag acgaccttgc acagcaggtc attggcggag gagtagtccg tctggccctg
     8341 gttgccgaaa cgccctgcga cagaggagaa gacgacgacc gcgccgacct ccatgtcctt
     8401 ggccgagcgc cacacgttga accacccggt ggtcttgacg tcgaagacca ggttgaactc
     8461 gcgcggctct ttctccggca ggttgcggct gatctccagc ccggccgcgt gcagcagcac
     8521 gtcgatgctg ccgctgcgct cgcgcacctg ctccatgacc tcagcgacat tgccggcgtc
     8581 ggtcaggtcg accgggtagt agaaggcctt gccgccggcg tcggtgaccg cctcgatcgc
     8641 ggtcagggcc gcctcgagcc gctcgatccg ggcgatctcg cggtcgatga ggaccggtgt
     8701 ggccttcttg ccctgctcct tcaggcgcgt ggcgatccgg gccttcagcg cggccttgtc
     8761 gtcgcggaag gtgttgatgt cctcgtcgtc aggatcgggt gaaggcgtca ggtcgagcag
     8821 gtggaaggtg ccgccgctgg ccttggccag gtccgccgtg atcgccgaga cgatgctgcc
     8881 cgcagcaccg gtgatgacga agacgctgtc cttgccgagc gtcataccgc cctcgccgtg
     8941 cggcgaaccg tccgggccga gcggggtgaa ggcccgctcg acgaaggcca cgccataacg
     9001 caggccatcc gccgcgcggc cgacctcgac acagccggga tcgcgcagcg tctcctcgat
     9061 gagcaggtcg gcgatggccg ccgtcttgcg gctggccggc aggtcgacgg ccttgacgag
     9121 ggcgtcgggc cgctccttct tgtaggactt cgcgaagccc gtcaccgcgc cgccgaggac
     9181 gctcgtcgcg ccgccggcgt cgtagccgtg gaacccgccg aggcgcgtcg cgacgacgag
     9241 gaaggggctg gtctcccaca gcgtgcgcat cgtggcatag agccccttga cgcggcgctg
     9301 cagcgcacct tgccactcgt tcagctcgta tgccgtgacg tcgccctcgt cgtccagcgc
     9361 cggcagccag tagacaccgg cgatgggtcc ttcggccatc cactcggcca agcggccggc
     9421 gaactcgtcg tcgctgttct ccgccgggcc gtaaagcact gtggcgccgg ccttttcgag
     9481 ccgcttgacg agggcgtcgg cgacgccgct gctctcgccg cgcacgacga cgcgggcgcc
     9541 cttcagctcc acgccggtgg ccacggactg ttccgcgctc gggcgcaggg ccggcacggg
     9601 tatgcggcgc ggcagcgcgt cgatcgcgcg caggtcgccc ttgatgagct tcccgtgggg
     9661 atgctccggg gcggccggtg cggcagccgc gggcgtggtc tcgccggcag ccgccggcgc
     9721 gccggccgca gctgcggagg gcgcggccat gccgagcttg tcgcggaccc agccggcgac
     9781 gtggttcatc gtcgggaagt cgcgcagctt gaggttctcg tcgcgttcga ggtcccagtt
     9841 gccgcggacg gcggcgaaga cctcggcctg cttgacggtg tcgacgccga ggtcggcttc
     9901 gaggtcgagc tcggggtcga gcagatcggc cggataaccg gtcatctccg cgacgatctc
     9961 ggtcaccttg gccaggacgg cgtccgagcc ggcagccggc gctgccgggg ctgcggc
//
LOCUS       DAOPJG010000128        10013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7071_length_10013_cov_8.459530, whole genome shotgun sequence.
ACCESSION   DAOPJG010000128 DAOPJG010000000
VERSION     DAOPJG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7071_length_10013_cov_8.459530"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(489..1412)
                     /locus_tag="PKU75_11480"
     CDS             complement(489..1412)
                     /locus_tag="PKU75_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPF81546.1"
                     /translation="MIRLGAKGEPSAKWTAEDIPDQSGRVFVVTGANSGIGFEAAKAL
                     CAKGAHVVFACRNVAKADEARALVPGSHETRALDLSDLDSVRAFADGVSDWRIETLVN
                     NAGIMAVPRATSAQGYELQFATNVLGHFLLTNLLLPRLTDRVVTLSSGVHRAGRLDLG
                     DPNFEGRRYTPWAAYSQSKLADLMFAYELQRRLTAAGSRLRSVAAHPGYTATNLQSKT
                     QTYQDRIMAVGNKIPLIAQSAAMGALPTLYAAIVPDLPGGTYVGPDSFRELRGHPRPV
                     GSSAASHDRTAAAGLWDLCERLTGQPFTVPT"
     gene            complement(1409..2605)
                     /locus_tag="PKU75_11485"
     CDS             complement(1409..2605)
                     /locus_tag="PKU75_11485"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPF81547.1"
                     /translation="MYGAVRDEILATLEEIRAAGLYKAERQLTTAQSSHIATTSGEAL
                     NFCANNYLGLADHPEVLAAARDALQEWGFGMASVRFICGTQAQHKELESALSEFLGTE
                     DTILYSSCFDANGGIFEVLFGEGDAIISDELNHASLIDGIRLCKAARYRYRNADMDDL
                     RAQLEATRQSGARRVVIVTDGVFSMDGSFAPLPAICDLADEFGALVMVDDSHAVGFIG
                     PTGAGTPQHFGVQERVDILSGTLGKALGGASGGYISSHREVVDLLRQRSRPYLFSNAV
                     APSVVAGSLAALRIASTASEGRIRLTANAALFRSLMGEAGFELLPGEHPIVPVMFRGE
                     DGARQATQIADAMLADGVYVIAFSYPVVPKGAARIRVQLSAAHSEDDVRACAAAFVAA
                     RDRFAG"
     gene            complement(2611..3657)
                     /gene="tdh"
                     /locus_tag="PKU75_11490"
     CDS             complement(2611..3657)
                     /gene="tdh"
                     /locus_tag="PKU75_11490"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPF81548.1"
                     /translation="MRALVKTGAHAGLEMMDVPEPPCGSLDVKVRVLRAGLCGTDLHI
                     QDWDDWAASTLEPPLIVGHEFYGEVVEVGEDVPETAVRVGDRISAEGHIVCGHCRNCR
                     AGRRHLCINTIGVGVNRDGAFADYVVIPAMNAYVQPEGMDPDLAAIFDPYGNATHTAL
                     QWPMVGEDVLITGAGPIGVIAVAIARHAGARNIVATDISDFRLDLAKGAGADLVVNTA
                     HTRIRDAQRELGMREGFDIALEMSGAPSAVQELIDNMNHGGRVALLGLPKTEYPVNWG
                     KVITHMLTIKGIYGREMFESWYAMGAMLQTSPELTRAVSSVITHRVPAAEWAQAFDIA
                     RSGQCGKVVMNWED"
     gene            3729..4121
                     /locus_tag="PKU75_11495"
     CDS             3729..4121
                     /locus_tag="PKU75_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF81549.1"
                     /translation="MIDHFGINCSDFEASKRFYDGVLGILGHSREMDFGAAIGYGSEG
                     KPAFWISKWPGAEPNREMHVAFAAPDLATVHAFYDKAMELGAESLHAPRLWPEYHPGY
                     YGAFVRDPDGNNLEAVCHDAPPEATAAS"
     gene            complement(4114..5019)
                     /locus_tag="PKU75_11500"
     CDS             complement(4114..5019)
                     /locus_tag="PKU75_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF81550.1"
                     /translation="MPNAEVNGTTICYEEHGSGEPMLLIMGLATQMIAWPPEMVSALV
                     DRGYRVIPFDNRDIGLSGKTHGPAPVLRDVLRGAASPRLARPDYHLADMADDTAALIA
                     HLDLPKAHIVGVSMGGMIAQEVAIRHPEQVATLASIMSNTGSRLQGKPAASFMNEFRK
                     SFLTPRPSSREQAVDFALNAWRQISGPHFDETEMRHMIELSVERDADPIGRVRQILAI
                     LAAPDRTAQLRQLDVPTVVIHGLLDRLVSPSGGIATARAIPGSRLLMFPDMAHDLPRP
                     RIGEIVDAIVTNAQRAPIGAARRAS"
     gene            5132..5527
                     /locus_tag="PKU75_11505"
     CDS             5132..5527
                     /locus_tag="PKU75_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPF81551.1"
                     /translation="MPHPPRYREDDPLLARVRGIALAMPGAKEKISHGTPSFYTVKIF
                     AQYGAAIKGDHDSWALRRSLVFLPDDQERPALEEDPRIHVPAYVGAYGWLALDLTAGE
                     PDWDEVRELLDASYRNTAPARLIRELDAT"
     gene            5556..6554
                     /locus_tag="PKU75_11510"
     CDS             5556..6554
                     /locus_tag="PKU75_11510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPF81552.1"
                     /translation="MSDSRTPLSILDLAFVRHGQPVAEALAQSVELAQHAEATGRFER
                     IWYAEHHNMPAIASSATAVLIAHVAARTERIRLGAGGVMLPNHAPLIIAEQFGTLAEL
                     HPGRIDLGLGRAPGTDQVTLRALRRDPRASDEFPSDIRELQGYLAGESLIPTVSAHPG
                     AGTRVPLYILGSSLYGAQLAAAFGLPYAFASHFAPDALHAAMRVYRERFQPSEQLAEP
                     YAVAALNVIAAAEGGAARELADRVLRSRVRNLAERVPAYAGRLDDAAVDALMTSPVAD
                     QARHMLTYTAVGDRDEVADYLRTFREQCGADELMITNPAPDLAARHTVLDILAAID"
     gene            6623..8887
                     /locus_tag="PKU75_11515"
     CDS             6623..8887
                     /locus_tag="PKU75_11515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPF81553.1"
                     /translation="MPNLPELLHAAVSDLGGTQRPGQTQMAQAVLDAVDKEEHLLVQA
                     GTGTGKSLGYLVPAILHAQRSGKPAIVATATLALQGQIIDRDLPRLATALAPILGRRP
                     TYALVKGRRNYVCLHKLEGGFPDEDEGLFDVGEADASAGRLGKEVVRLREWAQETESG
                     DRDELVPGVSERAWRQVSVAAEECLGSRCPMAGECFVERSRAAAKDVDIVVTNHSFLA
                     IDAFEGRFMLPEHELLVIDEGHELVDRITSTITDELTPGMVKRAAKRARSADSSDVLD
                     DTALVLERALEQLPEARIDAVPEQLALALATVRDAARTVQSELKPQPGEQIDGAKQVA
                     IAAVDEIFDNSARMLEERELDVLWNRVDQRRGAVLNVAPMSVAMLVRDKIFQDRTVIL
                     TSATLELGGTFDALAGTMGLRGEGAPAYTGLDVGSPFNYPEQAIAYVAATLPAPGRGG
                     LSDQMLDEIEALVRAAGGRTLGLFSSMRAAREAAEAMRDRLGESEISILCQGEDQIST
                     LVRDFARDPTTCLFGTLSLWQGVDVPGSSCQLVLIDRIPFPRPDDPLASARTEAIGRM
                     GGNGFMAVSATHAALRLAQGAGRLIRSASDRGVVAFLDSRMMTARYAGFLQRSLPPFW
                     PTTDRQLVLSALQRLDQTAPPPREVPKPGKRTLQGVAAEAAEKVDADGAAHAEPEKPT
                     APPPPPPENARTAVTSGHPWTEQDDEELRDGVEMGLSLDELAESLELPEEIIAARLQG
                     LGLEISPETQLSFD"
     gene            8989..9261
                     /locus_tag="PKU75_11520"
     CDS             8989..9261
                     /locus_tag="PKU75_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81554.1"
                     /translation="MSRGGTPVARGSIAAAAAVHALLLSIGSAGYGYHRDELYFRMLP
                     PRWSYLDQPPLTPFLARLTTHLADEPWALRIPATLRARSPSSCSPC"
     gene            9553..9963
                     /locus_tag="PKU75_11525"
     CDS             9553..9963
                     /locus_tag="PKU75_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81555.1"
                     /translation="MPWPWLGALPALVVAAPQIAYQAGHEQVATAYHRAGDADILTSN
                     YGEAGAVARFGPAYGLPAPVSGHNALADLTMPTRDRVLVLGGAWCDLAPAFARCERVG
                     ELDNGVDVDNEEQGEELTLCAGRTPPWADLWPHL"
BASE COUNT         1533 a         3651 c         3335 g         1494 t
ORIGIN      
        1 cccctccgcg cacctcacgt catcaaaccg ctgccccgtg aaagacggag gctaaccgcg
       61 gacctatttc cggagaggag ttgtcggtgt cgcgtcgtag tgtcgtggcg tgcatcccag
      121 ccgagtcggg gtccttctcg tcgacattcc ccaggagtcg tatgccgcag ccgccgcctt
      181 ctggtctgcg gcgcggggtc cgagcgtgag caaggagacg cgccgccggg ggaggatccc
      241 tgcgagatgc tcgccccctt atcgggtagg gaactcttcc aactgcagcg caccggcgcg
      301 ggcacgccac cgcgaatcca cctcgacatc gagaccgacg acgtgccggc cgaggtcgag
      361 cgcgtcctga agcttggggc gagtgtggtc cgagcagcgg cagggatacg ccatcatggc
      421 cgatcccgcc ggccggctct tctgcgtcgt ccccgtgcag accggcgccg acttcgagca
      481 ccacgcgatc acgtggggac ggtgaacggc tgcccggtga gccgctcgca caggtcccac
      541 aacccggcgg cggccgttcg gtcatgcgac gccgcgctcg accccaccgg ccgcggatgg
      601 ccgcgcaact cccggaaact atccggaccg acatacgtgc cgcccggcag gtcggggacg
      661 atcgcggcat acaaggtcgg cagtgcaccc atggccgccg actgggcgat cagcgggatc
      721 ttgttgccca cagccatgat tcggtcctga taggtctgcg tcttcgactg tagattggtc
      781 gcggtgtagc ccgggtgcgc agcgaccgac cgcagccgcg acccggcggc cgtcagccgc
      841 cgctgcagct cgtaggcgaa catcaggtcg gccagcttgc tctggctgta tgccgcccac
      901 ggcgtgtagc ggcgcccctc gaagttcggg tcgcccaggt cgagccggcc ggcccggtgc
      961 accccggagg acaaggtgac gacgcgatcg gtcagccgcg gcaacagcag gttggtcagc
     1021 aggaagtgcc cgagaacatt ggtcgcgaac tgcagttcgt agccctgcgc cgaggtggcc
     1081 cgcggcaccg ccatgatccc cgcgttgttg acgagcgtct cgatccgcca gtctgacacc
     1141 ccgtcggcga aggcccggac cgagtccaga tcggacagat cgagggcgcg agtctcgtgc
     1201 gagcccggca caagagcgcg cgcctcgtcc gccttggcga cattgcggca agcaaagacg
     1261 acatgagcac ccttggcgca cagcgctttt gcggcttcga agccgatccc ggagttggcc
     1321 ccggtcacga cgaacacccg accgctctgg tcggggatgt cctcagcggt ccacttcgcc
     1381 gacggctcgc ccttcgcccc cagccggatc aacccgcgaa ccgatcacgg gcagccacga
     1441 acgccgctgc gcaggctcgc acatcgtcct cgctgtgcgc cgccgacaac tgcacccgga
     1501 tccgtgccgc tcccttgggc accacgggat aggagaaggc gatgacatac acgccgtcgg
     1561 cgagcatcgc gtccgcgatc tgtgtcgcct gccgcgcccc gtcctcgccc cggaacatca
     1621 ccggcacgat cgggtgctca cccggcagca gctcgaaacc cgcctcgccc atcagcgacc
     1681 ggaagagggc tgcgttggcc gtcaaccgga tgcgcccctc cgacgcggtg ctcgcgatcc
     1741 gaagtgccgc aagcgaaccc gccaccacac tcggggccac cgcattggag aacagatacg
     1801 gacgcgagcg ctgacgaagc aggtcgacca cctcacgatg gctggagatg tatccgcccg
     1861 atgcgccgcc gagcgccttg cccagggtgc cgctgaggat gtctacgcgc tcctgcacgc
     1921 cgaagtgctg tggggtccca gcccccgtgg gcccgatgaa ccccacggcg tgggagtcgt
     1981 cgaccatcac cagcgcaccg aactcgtccg ccaagtcaca gatggccggc agcggcgcga
     2041 acgagccgtc catcgagaac acgccgtcgg tgacgatgac gacccgccgc gcgccggact
     2101 gccgagtcgc ctcgagctga gcgcgcaggt cgtccatgtc ggcgttgcgg tagcgatatc
     2161 tcgcggcctt gcagagccgg atgccgtcga tcagcgaggc gtggttcaac tcgtccgaga
     2221 tgatcgcgtc gccctcgccg aagagcacct cgaagatgcc gccattggcg tcgaaacagg
     2281 aggagtagag gatggtgtcc tccgtgccga ggaactccga cagggcgctc tcgagctcct
     2341 tgtgctgcgc ctgggtgccg cagatgaacc ggacgctcgc cattccgaaa ccccactcct
     2401 gcagggcatc ccgggcagcg gcgagcacct cgggatggtc ggccagaccc agatagttgt
     2461 tggcgcagaa gttcaacgcc tccccactcg tcgtcgcgat gtgcgacgac tgggcggtcg
     2521 tcagctgccg ctccgccttg tagagcccgg ccgcccggat ctcctcgagc gtcgccagga
     2581 tctcgtcgcg cactgcgcca tacatccgaa tcagtcctcc cagttcatga cgactttgcc
     2641 gcactgcccc gaccgggcga tgtcgaaggc ttgcgcccac tccgcggccg gcacccggtg
     2701 agtgatgacg gaggacacgg cgcgggtgag ttccggcgag gtctgcagca tcgcgcccat
     2761 ggcgtaccag gactcgaaca tctcacgccc gtagatcccc ttgatcgtga gcatgtgggt
     2821 gatgaccttg ccccagttca ccggatactc ggtcttgggc aggcccagca gggcaacccg
     2881 cccgccgtgg ttcatgttgt cgatcagttc ctggacggcg ctcggtgcac ccgacatctc
     2941 caacgcgatg tcgaagcctt cgcgcatccc gagttcgcgc tgcgcgtcgc ggatccgagt
     3001 atgggcagtg ttgacgacca ggtctgcccc cgcgcctttg gcgaggtcga gacggaaatc
     3061 ggagatgtcg gtggccacga tattgcgtgc gccggcgtgc cgggcgatcg cgaccgcgat
     3121 gaccccgatc gggccggcgc cggtgatcag cacgtcctca ccgaccatgg gccattgcag
     3181 cgcggtgtgc gtcgcgttgc cgtaggggtc gaagatcgcg gcaaggtccg gatccatgcc
     3241 ctcgggttgc acgtacgcgt tcatagcggg gatgacgaca tagtcggcga atgccccgtc
     3301 gcggttgacg ccgacaccga tcgtgttgat acacaagtgc cttcgcccgg cccggcagtt
     3361 ccggcagtgg ccgcacacga tgtggccctc ggcggagatg cgatccccga cgcgcacggc
     3421 ggtctccggc acgtcctctc cgacctcgac gacctcgcca tagaactcgt gccccacgat
     3481 cagcggcggc tcgagtgtcg aggcggccca gtcatcccag tcctggatgt gcagatcggt
     3541 gccacacagg ccggcccgca gcacccggac cttcacatcg aggctcccgc agggtggctc
     3601 gggaacgtcc atcatctcca ggcccgcgtg ggccccggtc ttcacgagcg cgcgcattgc
     3661 atccttcctc gggtggctgt tgtcagcgta tgccgcgggc cgcccgcagg ggtgggagga
     3721 tcggagccat gatcgatcac ttcgggatca actgctccga cttcgaggcc agcaagcgct
     3781 tctacgacgg cgtcctggga attctcgggc actccaggga gatggatttc ggggcggcca
     3841 tcggctatgg ctcggagggc aagccggcgt tctggatcag caaatggcct ggggcagaac
     3901 ccaaccggga gatgcacgta gcctttgcgg cgccggacct cgcgaccgtg cacgccttct
     3961 acgacaaggc gatggagctg ggcgccgaaa gcctgcacgc gccgcgactg tggcccgaat
     4021 accaccctgg ctactacggc gccttcgtgc gcgacccgga cggcaacaac ctcgaggccg
     4081 tctgccacga cgcgccaccc gaggcgaccg ccgctagcta gcccggcgcg ccgccccgat
     4141 cggggcccgc tgcgcgttgg tgacgatggc gtcgacgatc tcgccgatac gcggacgagg
     4201 caggtcgtgc gccatgtccg ggaacatcag cagccgcgag ccggggatcg cccgggccgt
     4261 cgcgatcccg ccgctagggg agacgaggcg atcgaggagg ccgtggatga ccacggtcgg
     4321 cacgtcgagc tgccgcagct gggcggtgcg gtccggggcc gcgaggatgg cgagaatctg
     4381 gcggacccgc ccgatcgggt cggcatcccg ctcgacggac aactcgatca tgtgccgcat
     4441 ctccgtctcg tcgaagtgcg gaccggagat ctgccgccac gcgttgagcg cgaagtcgac
     4501 ggcctgctcc cgcgagctgg ggcgcggggt gaggaagctc ttgcggaact cgttcatgaa
     4561 ggaggccgcc ggcttgccct gcagccggct gccggtgttg gacatgatcg aggccaaggt
     4621 cgccacctgc tccgggtgtc ggatcgcgac ctcttgcgcg atcatgccgc ccatcgacac
     4681 tccgacgatg tgggccttcg gcaggtccag gtgcgcgatg agcgcggcgg tgtcatcggc
     4741 catgtcggcc aggtggtagt cgggccgcgc cagccggggc gaggcggcgc cgcgcagcac
     4801 atcgcgcagg acgggagcgg gtccgtgagt cttaccggac aatccgatgt cgcgattgtc
     4861 gaacgggatc acgcgatacc cccgatcgac cagggcgctg accatctccg gcggccaggc
     4921 gatcatctgg gtcgccagac ccatgatgag cagcatcggc tccccgctgc cgtgctcctc
     4981 gtagcaaatg gtggtcccgt tgacctcggc gttcggcatc cttcgattca atcaccatcg
     5041 tgacgggtgg ggctcgacgc gtcgtgccac tcccgtcaca cgcaacggcc cgacacgcgc
     5101 ccggtcccgc gctcctggca ggcttggccg tatgccgcac ccgccccgtt atcgcgagga
     5161 cgaccccctc ctggcacgcg tgcgcgggat cgcgttggcc atgcccgggg cgaaggagaa
     5221 gatctcgcac ggcacgccga gcttctacac cgtcaagatc ttcgcccagt acggcgcggc
     5281 catcaaaggc gaccacgact cgtgggccct acgccgctcg ctcgtcttcc ttcccgacga
     5341 ccaggagcgg ccggcgctcg aggaggaccc ccgcatccac gtgcccgcgt atgtcggtgc
     5401 ctacggttgg ctggccctcg acctgaccgc gggggagccg gactgggacg aggtccgaga
     5461 actcctggac gcctcatacc gcaacacggc gccggcgagg ctcatccgcg aactcgacgc
     5521 cacctgatcg ccccggcgtg gcatacggtt gcggggtgag cgactcgagg accccgctgt
     5581 ccatactcga cctggcgttc gtgcgccacg gccagccggt cgccgaggcg ctggcgcagt
     5641 cggtcgaact ggcgcagcac gcggaggcga ccgggcgctt cgagcggatc tggtatgccg
     5701 agcaccacaa catgcccgcc atcgcctcct cggccacggc ggttctcatc gcccatgtcg
     5761 cggcccgcac cgaacggatc cgcctcggcg cgggcggggt catgctgccc aaccacgccc
     5821 cgctcatcat cgccgagcag ttcggcaccc tggccgagtt gcatccggga cggatcgacc
     5881 tgggcctcgg gcgcgccccg ggcaccgacc aggtcaccct ccgcgccctg cgtcgcgacc
     5941 cgcgcgcctc cgacgaattc ccttccgaca tcagggaatt gcagggttat ctcgccgggg
     6001 agtccctgat cccgaccgtg tcggcgcacc caggcgcggg cacgcgggtg ccgctctaca
     6061 tcctcgggtc ttcgctgtat ggcgcgcagc tggccgccgc gttcgggttg ccgtatgcct
     6121 tcgcgagcca cttcgcgccc gacgcgttgc acgcggcgat gcgggtctat cgggagcgct
     6181 tccagccctc cgagcagctc gccgagccgt atgccgtcgc cgcgctcaac gtcatcgccg
     6241 cggctgaggg cggggcggcc cgcgagctgg cggatcgggt gctccgctcc cgggtgcgca
     6301 atctcgccga gcgggtcccg gcatacgccg gacgactgga cgacgcggcg gtcgacgcgc
     6361 tgatgacctc ccccgtcgcg gaccaggccc ggcacatgct cacctacacg gcggtggggg
     6421 atcgcgacga ggtggccgat tatctgcgca ccttccgcga gcaatgcggg gccgacgagc
     6481 tgatgatcac caacccggcc cccgacctcg ccgcgcggca tacggtcctc gacatcctcg
     6541 cggccatcga ctgacggtcc gcctcgccgg gggtgtggat aaggcccggg cggtgtcggc
     6601 ccgcggacat aggctggccg gtatgccgaa tctccccgag ctcctgcacg ccgcggtcag
     6661 cgacctcggc ggcacgcagc ggccgggtca gacccagatg gcgcaggcgg tgctcgacgc
     6721 cgtcgacaag gaggagcacc tgctcgtcca ggccggcacc ggcacgggca agtccctcgg
     6781 ctacctcgtc ccggcgatcc tgcacgcgca gcgcagcggc aagcccgcca tcgtcgccac
     6841 ggccaccttg gccctccagg ggcagatcat cgatcgcgac ctgccgcggc tggccaccgc
     6901 cctggccccg atcctcgggc ggcgcccgac ctatgccctg gtcaagggcc gccgcaacta
     6961 tgtctgcctg cacaagctcg agggcggttt ccccgacgag gacgagggcc tgttcgacgt
     7021 gggggaggcc gatgcctccg cgggccggct cggcaaggag gtcgttcgcc tgcgcgaatg
     7081 ggcccaggag accgagtccg gtgaccgcga cgagctcgtt ccgggtgtgt ccgagcgggc
     7141 ctggcgccag gtcagcgtcg ccgccgagga gtgtctcggc tcgcgctgcc cgatggccgg
     7201 cgagtgcttc gtcgaacgct cccgggccgc cgccaaggat gtcgacatcg tcgtcaccaa
     7261 ccactccttc ctcgcgatcg acgccttcga gggccgcttc atgctgcccg agcacgaact
     7321 gctcgtcatc gacgaggggc acgagctcgt cgaccggatc acctccacca tcaccgacga
     7381 gctgaccccc ggcatggtca aacgggccgc caagcgcgcc cgctccgccg actccagcga
     7441 cgtcctcgac gacaccgccc tcgtgctcga aagggcgctg gagcaactgc ccgaggcgcg
     7501 gatcgacgcc gttcccgagc aactcgcgct cgccctggcg acggtccgcg acgccgcccg
     7561 caccgtccag tccgagctga aaccgcaacc gggcgagcag atcgacggcg ccaaacaggt
     7621 ggccatcgcc gccgtcgacg agatcttcga caacagcgcc cgcatgctcg aggagcgcga
     7681 actcgacgtc ctgtggaatc gcgtcgatca gcgccggggc gccgtcctca acgtcgcacc
     7741 gatgagcgtg gcgatgttgg tgcgcgacaa gatctttcaa gaccgcaccg tcatcctcac
     7801 ctcggccaca ctcgagctcg gcggcacctt tgatgccctc gccggcacga tggggctacg
     7861 gggcgagggc gcaccggcct acaccgggct cgatgtcggc agccccttca actaccccga
     7921 gcaggcgatc gcctatgtcg cggcgacgct gccggcgccc ggtcgcgggg gcctcagcga
     7981 ccagatgctc gacgagatcg aggcgcttgt ccgggccgcc ggcgggcgca ccctcggcct
     8041 cttctcctcc atgcgcgccg ctcgcgaggc cgccgaggcc atgcgagatc gcctgggaga
     8101 gagcgagatc agcatcctgt gccaggggga ggaccagatc tccacgttgg tgcgtgactt
     8161 cgctcgcgac ccaacaacct gcctgttcgg caccctcagc ctgtggcagg gcgtcgacgt
     8221 gcccggctcg tcctgccagc tcgtcctcat cgaccgcatc cccttcccac gccccgacga
     8281 tccgctggcg tcggcccgca ccgaggccat cggacggatg ggcggcaacg gcttcatggc
     8341 cgtctccgcc acccacgccg ccctacgcct ggcccagggc gccggccggc tgatccggtc
     8401 cgcgagcgat cgcggtgtgg tcgccttcct cgactcgcgc atgatgaccg cgcggtatgc
     8461 cgggttcctc caacgctcgc tgccgccgtt ctggccgacg accgaccggc aactggtgct
     8521 ctctgcgctg cagcgcctcg accagaccgc cccgccgccg cgcgaggtcc ccaagcccgg
     8581 caagcgcacc ctgcagggcg tcgccgccga ggccgcagag aaggtcgacg ccgacggcgc
     8641 cgcccacgcc gaaccggaga agccgaccgc cccgccgcca ccgccgccgg agaacgcgcg
     8701 cactgccgtc acgagcgggc acccgtggac cgagcaggac gacgaggaac tgcgcgacgg
     8761 cgtcgagatg ggcttgagcc tcgacgagct cgccgaatcc ctcgaactgc ccgaagagat
     8821 catcgccgcc cgccttcagg ggctcggctt ggagatctcg cccgagacgc agctgtcgtt
     8881 cgactgatcc cggttccgat cccggtggtg cggttgacga tcacaaccgg gtgacaaccc
     8941 cgcgaagacc ctcccgcgca gcggccgagc cacccgtatg gtggacgcgt gagccgtggg
     9001 ggaacaccgg tcgcgcgggg gagcatcgcc gcggcggccg cggtgcacgc tctcctcctg
     9061 agcatcggaa gcgccggcta cggctaccac cgcgacgagc tctacttccg gatgctgccg
     9121 ccgcgctggt cctacctcga ccagccgccg ctgaccccct tcctcgcccg gctgaccacc
     9181 cacctcgccg acgaaccctg ggcgctgcgc atcccggcca ccctgcgagc acgctcgccg
     9241 tcgtcctgct cgccctgctg acacaccggc tcggcggcgg catcgcggcc cagcggctgg
     9301 cggcctgggg gtatgccgtg gccgccttcc cgctcatgct cggccacctg ctgctcacct
     9361 cgacgatcga cctgcccatc ctcctcggcg tggtcctcct tgtcgtcaaa gccgttgacg
     9421 ggcaaccgaa atcgtggctg gcggcgggag tgctcgcggg cgcggcggca tacaaccggc
     9481 tcctggtcgt cgccctgctg gcgggtcctc gtcgcggggt tgctcgcgct cggcccgcgc
     9541 gccacgctgc gtatgccgtg gccatggctc ggcgcgctgc ccgccctcgt cgtcgcggct
     9601 ccgcagatcg cgtaccaggc cggccacgag caggtggcca ccgcatacca ccgcgcgggc
     9661 gacgccgaca tcctgacgtc gaactacggt gaggccggcg cggtcgcccg gttcggaccg
     9721 gcatacggcc tgcccgcacc ggtgagcggg cacaatgcgc tcgccgatct gacgatgccg
     9781 acgcgggacc gggtgttggt cctcggaggg gcgtggtgcg acctcgcccc ggccttcgct
     9841 cgctgcgagc gggtgggcga actcgacaac ggcgtggacg tcgacaacga ggagcaggga
     9901 gaagaactca cgctctgtgc cggccgcacc ccaccctggg cggacctctg gccgcacctc
     9961 tagctcacct ggactgaccc ttcgcggctg ggctgtcggc ccggcgtggc agg
//
LOCUS       DAOPJG010000129         9933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7137_length_9933_cov_7.168860, whole genome shotgun sequence.
ACCESSION   DAOPJG010000129 DAOPJG010000000
VERSION     DAOPJG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7137_length_9933_cov_7.168860"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1030)
                     /locus_tag="PKU75_11530"
     CDS             complement(2..1030)
                     /locus_tag="PKU75_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81556.1"
                     /translation="MTQRTGARRLVTSAATTGLIAGAALTSAQAASASGADAASAPAK
                     AAPAYISLLFGRANWQVAGGRDCSITFPGARTLEQNAQDMKARGVTGTAMVVVGRTVD
                     TGHSCFGGYTLQSSWEDLANLRSEYGWQVTSQGMYYENMTLMTTDAQRYAESAATLPI
                     FRDHGFTRAWGSFAFANNKMDLPAQKLVSKYFAFLRKYGDGVTRKAKATTFPYIMTTL
                     SINGGQCNNVNLKCYDFPVANDRRATDLNRLKTLMNPAPDTWSVVQAYRFVEGKQGAM
                     PTQPTPQVFSWDCTSSDWRNRWTSQPEISCRNTYLEMLDARSPSAKVTDPATVARAWG
                     RIPPLTAK"
     gene            complement(1095..2042)
                     /gene="metA"
                     /locus_tag="PKU75_11535"
     CDS             complement(1095..2042)
                     /gene="metA"
                     /locus_tag="PKU75_11535"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HPF81557.1"
                     /translation="MPVTIPSALPARRILEGENVFVMTEGRAGHQDIRPLRIAIVNLM
                     PTKMATETQLLRLLGNTPLQLEITLIRMASHDPRNTAQAHLDAFYTTFEEARGDKFDG
                     LIITGAPIETLPFEAVDYWPELCEIMDWATDHVYSTLYVCWGAQAGLFHHYGIEKYPL
                     AHKMFGVFAHRVLDPRNRVLSGFDEVFPAPHSRHTEIRASDVEATDGLQLLAVSDEAG
                     VYLAASTDGRHLYVTGHPEYERDTLKAEYDRDIGRGLDIAVPRHYYPGDDPTQPPMVT
                     WRSHAFLLYANWLNYCVYQGTPYDLAAISSHTAPPSDED"
     gene            complement(2092..2535)
                     /locus_tag="PKU75_11540"
     CDS             complement(2092..2535)
                     /locus_tag="PKU75_11540"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPF81558.1"
                     /translation="MASLAPGGRIAVVAAALLDDLTAPTQLLSAQRTEPPLLAGGWEI
                     PGGKVDPGEEPLDALHREIHEELGVRIEVGDGIPGPLDGWWPLGERYAMLVWLARVSD
                     GIPQPLEGHSQLRWLPEHALWSVDWLPANRPILAAIAATLWTPEH"
     gene            complement(2535..3968)
                     /locus_tag="PKU75_11545"
     CDS             complement(2535..3968)
                     /locus_tag="PKU75_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HPF81559.1"
                     /translation="MNAASQVVIIGGGPGGYEAALVAAHLGAQVTMIDRDGLGGAAVL
                     TDCVPSKTLIATADYMSMVDVAIDLGVRMHDHEGDVISDASAELAQINRRVLDLAAAQ
                     SEDIERRLDEVGVTIVRGTGRLVDASTVRAELSSGTEGLHRTQDFHADVVLVSTGATP
                     RVMGTAMPDGERILTWQQIYDLTEMPEHLIVVGSGVTGAELAQAYLGLGAQVTLVSSR
                     QHVLPGHDPDAARVIEDVFTKRGMQVLSRSRMQATDVIRDESGQATGVTVTLEDGRVI
                     EGTHALLAVGAIPQTRGLGLEEAGVALSESGHIRVDRVSRTSVRGVYAAGDCTGVLPL
                     ASVAAMQGRIAIAHALGDAVSPLNLDVVSANIFTDPEIATVGLSEKDIDDKALEVATV
                     MLSLSRNPRAKMQGITDGFVKLFAAPGTGTVLGGVVVAPSASELIFPVTLAVSHRLNV
                     DQLASAFTVYPSLTGSIAEAARRLHAT"
     gene            4096..4929
                     /locus_tag="PKU75_11550"
     CDS             4096..4929
                     /locus_tag="PKU75_11550"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPF81560.1"
                     /translation="MDTAVNPLEAPDTDPFAIATVAAAVIADTSGSATHDIALVLGSG
                     WGETAERIGATLATIDNTDVPGFAKAAVVGHSGTMRSVAIGDTGRRALVFGTRTHFYE
                     GRGVRSVVHAVRTAAAAGCRTIVLTNGCGGLNPDWAPGTPVLIRDHINLTAASPLEGA
                     TFVDLTDLYSPRLRALAREIDPGLPEGVYVQFRGPQYETPAEVKMAGILGGDLVGMST
                     TLEAIAARQAGLEVLGISLVTNPAAGISPTPLDHAEVVAAGRAAAARCGDLLAQIVTR
                     I"
     assembly_gap    4933..5032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5054..6715
                     /locus_tag="PKU75_11555"
     CDS             5054..6715
                     /locus_tag="PKU75_11555"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPF81561.1"
                     /translation="MPSAPDLLDAARAWAADDPDPQTRAELEAVIAAASEGDEEAVAD
                     LADRFSGMLEFGTAGLRGALGAGPNRMNRAVVIHAAAGLTAYLKSVAPEPFVVIGFDA
                     RTNSDVFARDTAAVVCGAGGRAAVLPRPLPTPVLAYAIRFLGADAGVMVTASHNPPQD
                     NGYKVYVGDGSQIVPPVDGLIAAAIAGVESVASVPMADDGWETLTDDVLDAYVADIAT
                     IVSPLAPRELTVVHTAMHGVGTETIQRAFAAANFPQPISVAAQAEPDPMFPTVSFPNP
                     EEPGAMDLALDLAEQTGPDLVIANDPDADRCAAAIPGPGGWRMLRGDEVGALLGAHIL
                     DRGVPEGGRFANSIVSSRLLGAMAAKAGVSHEETLTGFKWIGRVPSLAFGYEEALGYC
                     VDAAHVRDKDGISAALLLAELAAERKADGSDLQTVLDDLAIEHGVYATDAFSVRVDDL
                     SLIGAIMARLRATPLTEVGGIPVARQQDLAVGVDGLLPTEGLRYYLGDDSRIIVRPSG
                     TEPKLKIYLEVIEPVDDAADLSQARTRAAGRLSGIRAAMEATTRP"
     gene            complement(6826..8097)
                     /locus_tag="PKU75_11560"
     CDS             complement(6826..8097)
                     /locus_tag="PKU75_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPF81562.1"
                     /translation="MTRALLAVTVAVAGLSACTGPKETDPSAATDQLEVMTWWTSASE
                     AKAFKVFTDAYAGANAGVTLTNASVQGGAGSSARVALAKRLIAGDPPDVWQTFAGAAT
                     TAYEKSAQIRDVSSVFGAGGLAAQVNPTILQSVTIDGKQYAVPTGAHRSNVLFFNKAV
                     LDKAGVAAPAAGYTPAAFRADLEKVKASGVPALCLGAKDRFTTAELLDNILLARLGPD
                     GWAKIQDDKLDWNGTEVKDALGELDDLLAYTSADADTQTWDQAIGQLVEGSCGFLTMN
                     DSAFGELTARGGSDAVGEVVYPGTDGAFLAVTDVFVASRTAPNAKNALAFLSVLADPA
                     ANLEFSKLKGSVPVLKNVDATGLSPYQQEAAKALWAGPVLPSLSHGQAMNPGLTQGYF
                     DAIAAYVRTRQPQSFAQAIAEAVAVYQPAGH"
     gene            complement(8112..9440)
                     /gene="fucP"
                     /locus_tag="PKU75_11565"
     CDS             complement(8112..9440)
                     /gene="fucP"
                     /locus_tag="PKU75_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HPF81563.1"
                     /translation="MSQTPVKEASDARATKDPIVGPGMLVLFVVLVACFTAWGIAADL
                     TTPMVAGFKQIFEMSTFQASLVQLAYFGAYFLLALPAAFINKRFGYKVGLLTGLALAA
                     IGAFAFYPASKIMTYEAFLVALFAMAAGCSILETSANPYVIALGPESTATRRLNFAQS
                     FNPLGTNIGVLLASTLILPKLEAPVGVGALTPAEQQAIRAGQLGAVMGPYLGLGFLLL
                     VIWLVIAIQKSPPIHEEFHPTSEGETEQSVIKTLWGRRRYRFGVVAQFFNVAAQVCCW
                     TYILQYVQQALNGSLELGGYLLQVSLIVFMLSRFLMTWVIGKIRATKVLAALGTLAIV
                     LCLIAVVSPNMVGVAAIVAVSFCLSLMFPTIYGVALQGLGPATKFGAAGLVMAIVGGA
                     IMPLVQGIVIDKTSAAVSFIVPACCFAVVTAFALYDLKAPGGSAELASEA"
BASE COUNT         1531 a         3295 c         3457 g         1550 t
ORIGIN      
        1 cctacttcgc ggtcaacggc gggatgcgtc cccacgcccg cgcgacggtc gccgggtcgg
       61 tgaccttggc gctgggactg cgggcgtcga gcatctccag ataggtgttg cgacacgaga
      121 tctcgggctg gctggtccac cggttgcgcc agtcgctgct cgtgcagtcc caggagaaga
      181 cctgcggcgt cggctgggtg ggcatggcgc cctgcttgcc ctcgacgaac cggtacgcct
      241 ggaccaccga ccaggtgtcg ggcgccgggt tcatcagggt cttgagccgg ttgagatcgg
      301 tcgcgcgccg gtcgttggcc acggggaagt cgtagcactt caggttcacg ttgttgcact
      361 gcccgccgtt gatcgacagc gtcgtcatga tgtaggggaa ggtcgtcgcc ttcgccttgc
      421 gggtgacgcc gtcgccgtac ttgcgaagga aggcgaagta cttcgagacc agcttctgcg
      481 ccggcaggtc catcttgttg ttcgcgaacg cgaacgagcc ccacgcgcgg gtgaatccgt
      541 ggtcgcggaa gatcggcagc gtggccgcgc tttcggcata ccgctgcgcg tcagtcgtca
      601 tcagcgtcat gttctcgtag tacataccct gtgaggtgac ctgccagccg tactcggagc
      661 gcaggttggc caggtcctcc cacgacgact ggagggtgta gccgccgaag caggagtggc
      721 cggtgtcgac cgtgcggccg acgacgacca tggccgtgcc ggtgactccg cgggccttca
      781 tgtcctgggc gttctgctcc agggtgcgcg cgccggggaa ggtgatcgag cagtcccgcc
      841 cgccggcgac ctgccagttg gcgcgcccga agagcagaga gatgtatgcc ggggccgcct
      901 tcgcgggtgc gcttgcggcg tccgcgccgc tcgccgaggc cgcctgtgcg ctggtcaggg
      961 cagcgcccgc gatcagaccg gtcgtggccg cggaagtcac gaggcgacgt gcccccgtac
     1021 gttgagtcat ggatgctagt aaagccgacg acgcagcgaa acgcctagtc ctccggtgtg
     1081 cggacacgcc cgcgtcagtc ttcgtcggac ggcggggcgg tgtgggagga gatggccgcc
     1141 agatcgtatg gcgtgccctg gtagacgcag tagttgagcc agttggcgta gagcaggaag
     1201 gcgtgcgagc gccaggtcac catcggcggc tgcgtcgggt cgtcgccggg gtagtaatgc
     1261 cgcgggacgg cgatgtccag gccccgcccg atgtcgcggt cgtattcggc cttgagggtg
     1321 tcccgctcgt attcggggtg gccggtcacg tagaggtggc gcccgtcggt gctcgccgcg
     1381 agatacaccc ccgcctcgtc gctgaccgcg agcaactgca gcccgtcggt cgcctcgacg
     1441 tcggacgccc ggatctcggt atgccgtgag tgcggtgccg ggaagacctc gtcgaagccg
     1501 ctcagcaccc ggttgcgcgg atcgaggacg cggtgggcga agacgccgaa catcttgtgc
     1561 gccaacggat atttctcgat gccgtagtgg tggaagaggc cggcctgcgc gccccagcag
     1621 acatacaggg tcgagtagac gtgatccgtc gcccagtcca tgatctcgca cagctcgggc
     1681 cagtagtcga cggcctcgaa cggcagcgtc tcgatgggtg cacccgtgat gatgagcccg
     1741 tcgaacttgt cgccgcgggc ctcttcgaag gtcgtataga aggcgtcgag gtgggcctgg
     1801 gcggtgttgc gcggatcgtg gctcgccatc cggatcagag tgatctcgag ttggaggggg
     1861 gtattgccga gcagccgcaa cagctgggtc tcggtcgcca tcttggtcgg catcaggttg
     1921 acgatggcga tgcgcagcgg ccggatgtcc tggtggccgg cgcggccctc ggtcatgacg
     1981 aagacgttct cgccttcgag gatgcgccgg gccgggaggg cactcgggat ggtgacgggc
     2041 acgcgctgaa tccttccgtg atcagccgcc cattgtcgtc gcggggtccg gtcagtgctc
     2101 gggcgtccag agtgtggccg cgatggcggc caggatcggc cggttggcgg ggagccagtc
     2161 gaccgaccac agggcatgct cgggcagcca gcgcagctgc gaatgtcctt ccagcggttg
     2221 gggtatgccg tcgctcaccc gcgccagcca cacgagcatc gcgtagcgct cgccgagggg
     2281 ccaccacccg tcgagcgggc cgggtatgcc gtcccccacc tcgatccgta cgccgagttc
     2341 ttcgtggatc tcccggtgga gcgcatcgag cggctcctcg cccgggtcca ccttcccgcc
     2401 ggggatctcc cagccgcccg ccagcagcgg cggctccgtg cgctgggcgc tgagcaactg
     2461 ggtcggcgcc gtgagatcgt caagaagcgc cgccgcgacg acggcgatcc gcccgccagg
     2521 ggcgaggctc gccatcacgt cgcgtggaga cggcgggccg cctcggcgat cgagccggtc
     2581 aggctcggat agaccgtgaa agcgctggcg agctggtcga cgttgaggcg gtgggagacg
     2641 gcgagggtga cggggaagat cagctccgac gctgacgggg cgacgacgac gccgccgagc
     2701 accgtgccgg tgccgggtgc ggcgaacagc ttgacgaacc cgtcggtgat gccttgcatc
     2761 ttggcgcgcg ggttgcgcga gagcgacagc atgacggtcg cgacctcgag ggccttgtcg
     2821 tcgatgtcct tctccgacaa gccaaccgtc gcgatctcgg ggtcggtgaa gatgttcgcc
     2881 gagacgacgt cgaggttgag cggagacacc gcgtcgccga gagcgtgggc tatggcgatg
     2941 cggccctgca tggctgccac ggaggcgagg ggcagaacgc cggtgcagtc gcccgcggca
     3001 tacacgcccc ggacgctggt gcgcgagacc cggtcgacgc ggatgtgccc gctctcggag
     3061 agcgcgacgc cggcctcctc cagcccgagg ccgcgggtct gcgggatcgc gcccaccgcg
     3121 agcaaggcgt gcgtgccctc gatgacgcgg ccgtcctcga gggtgacggt gacgccggtc
     3181 gcctgccccg actcgtcccg gatcacgtcg gtggcctgca tccgggagcg gctcaggacc
     3241 tgcattccgc gcttggtgaa gacgtcctcg atgacgcgcg ccgcgtccgg gtcatggccc
     3301 gggaggacat gctggcggct cgagacgagg gtgacctgcg cgccgaggcc gaggtaggcc
     3361 tgggcgagct cggcaccggt gacaccggag ccgacgacga tcagatgctc gggcatctcg
     3421 gtgaggtcgt agatctgctg ccaggtgagg atgcgctcac cgtcgggcat cgcggtgccc
     3481 atgacgcggg gcgtggcacc ggtcgagacg agcacgacgt cggcgtggaa gtcctgggtg
     3541 cggtgcaaac cctcagtgcc gctggacaat tcggcgcgca cggtgctggc gtcgacgagg
     3601 cggccggtgc cgcgaacgat ggtgacgccg acctcgtcga ggcgccgctc gatgtcctcg
     3661 ctctgggcgg cggcgaggtc gaggacgcgc cggttgatct gggcgagctc tgcgctggcg
     3721 tcggagatga cgtcgccctc gtggtcgtgc atccgcacac cgaggtcgat ggcgacgtcg
     3781 accatcgaca tgtagtcggc cgtggcgatg agggtcttgg acggcacgca gtcggtgagc
     3841 accgcggcac cgccgaggcc gtcacgatcg atcatggtga cctgggcacc gagatgggcg
     3901 gcgacgagag cggcctcgta gccgccgggc ccgccgccga tgatgacgac ctgacttgct
     3961 gcgttcatac ccgccttgcc ctcgctcgat ccgtttccgg atgcccgttt cgagaggcca
     4021 gtctggccga ggcgccgcct tttcctcgaa tccgtcggcc cgcccggcga ggcgtgccga
     4081 taacctgcgc gggtgatcga caccgccgtg aaccccctcg aggcccccga caccgacccc
     4141 ttcgcgatcg ccacggtcgc cgccgcggtc atcgccgaca cgtccgggtc ggcgacccac
     4201 gacatcgccc tggtcctcgg gtcggggtgg ggcgagacgg cggagcggat cggcgcgacg
     4261 ctcgcgacga tcgacaacac ggatgtgccc ggtttcgcca aggccgcggt cgtcggccac
     4321 tcgggcacga tgcgttcggt cgcgatcggc gacaccggcc gccgcgccct ggtgttcggc
     4381 acccgcaccc acttctacga gggccggggg gtgcgctccg tcgtccacgc cgtccggacc
     4441 gcggcggccg cgggctgccg cacgatcgtg ctgaccaatg gctgcggcgg gctcaacccc
     4501 gactgggcgc ccggcacgcc ggtcctgatc cgcgaccata tcaacctcac ggccgcctcc
     4561 cccttggagg gcgcgacctt cgtcgacctg accgatctct attcgccgcg gctgcgggct
     4621 ctggcccgcg agatcgaccc cggcctcccc gagggcgtct acgtgcagtt ccgcggcccg
     4681 cagtacgaaa ccccggccga ggtgaagatg gccggcatcc tcggcggcga cctggtcggc
     4741 atgtcgacca ccctggaggc catcgccgcc cgtcaggccg gcctggaggt cctcggcatc
     4801 tcgctggtca ccaacccggc cgccggcatc tcccccaccc ccctcgacca cgccgaggtc
     4861 gtcgccgccg gccgcgccgc cgccgcccgt tgcggcgatc tcctcgccca gatcgtcacc
     4921 cgtatctgac ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcagcacc
     5041 caaggagtca ggtatgccgt ccgccccaga tctgctcgac gcggcccgcg cgtgggcggc
     5101 cgacgacccc gacccgcaga cccgcgccga actggaggcg gtgatcgccg cggcatccga
     5161 aggcgacgag gaggcggtgg cggacctggc cgaccgcttc tctgggatgc tggagttcgg
     5221 cacggccggg ctgcgcggcg ccctcggtgc cgggccgaac cggatgaacc gcgcggtggt
     5281 gatccacgcg gcggcggggt tgacggcata cctgaaaagc gttgcgccag aaccctttgt
     5341 cgtcatcggg ttcgacgccc gcaccaactc ggatgtcttc gcgcgcgata ccgcggcagt
     5401 cgtatgcggt gccgggggcc gggccgcggt gctgccccgg ccgctgccga cgccggtgct
     5461 cgcctatgcg atccggttcc tcggcgcgga cgcgggcgtc atggtcaccg cgagccacaa
     5521 cccgcctcag gacaatggct acaaggtgta tgtcggtgac ggctcgcaga tcgtgccacc
     5581 ggtcgacggc ctcatcgcgg cggcgatcgc cggggtcgaa tccgtggcat ccgtgccgat
     5641 ggccgacgac ggctgggaga cgctcaccga cgacgtcctc gacgcctacg tcgccgacat
     5701 cgccaccatc gtctcccccc tggcgccgcg ggaactgacc gtcgtccata cggccatgca
     5761 cggcgtcggc accgagacga tccagcgcgc cttcgccgca gcgaacttcc cccagccgat
     5821 atccgttgcg gcccaggccg aaccagaccc gatgttcccc accgtgtcct ttcccaaccc
     5881 cgaggagccg ggagcgatgg acttggccct cgatctggcc gaacagaccg gacccgacct
     5941 tgtcatcgcc aacgacccgg acgccgaccg ctgtgccgcc gcgatccccg gcccgggggg
     6001 ctggcggatg ttgcgcggcg acgaggtcgg ggcgttgctc ggggcgcaca tcctcgatcg
     6061 cggcgtgccg gagggcgggc gcttcgccaa ttccatcgtc agctcccgcc tcctcggggc
     6121 catggcggcg aaagccggtg tgtcccatga ggaaacactc acgggcttca aatggatcgg
     6181 acgcgtgccc agcctggcat tcgggtatga ggaggccctg ggctattgcg tcgacgcggc
     6241 acacgtgcgc gacaaggacg gcatcagcgc ggccctgctg ctggccgaac tcgcggccga
     6301 gcgcaaggcc gacggctccg acctccagac ggtcctcgac gatctcgcga tcgagcatgg
     6361 cgtctacgcc accgacgcct tctccgtgcg ggtcgacgat ctctccctca tcggcgcgat
     6421 catggcccga ctccgcgcga ccccgctgac cgaggtcggc ggcatacccg ttgcgcgaca
     6481 acaggatctg gccgtcggtg ttgacgggct gctgcccacg gagggactgc gctattacct
     6541 cggcgacgac agccgcatca tcgtgcggcc gtccggcacg gagccgaagc tgaagatcta
     6601 tctcgaggtg atcgagcccg tcgatgacgc ggccgacctg tcgcaggccc gcacccgcgc
     6661 cgccggtcgc ctctccggca tccgtgccgc catggaggcc accacccgcc cgtgacgccg
     6721 ccgccgctcg cgggtcaggg gctcttccat cgagatccca aggacatctt cctgacgagg
     6781 aagacccgcc tcggagggtt cgggcaagac cattgcgcac cccgctcagt ggcccgcggg
     6841 ctggtacacc gcgacggcct ccgcgatggc ctgggcgaag gactgcggtt gccgggtgcg
     6901 cacgtaggcc gcgatggcgt cgaaatagcc ttgtgtgagg ccgggattca tcgcctgacc
     6961 gtggctgagc gagggcagca ccgggccggc ccacagcgcc ttggccgctt cttgctgata
     7021 agggctgagg ccggttgcgt cgacgttctt caggaccggc accgaacctt tcagcttgct
     7081 gaactccaga ttcgccgccg ggtcggcgag gacggagagg aaggcgagcg cgttcttggc
     7141 attgggcgcg gtccggctgg cgacgaagac gtcggtgacg gcgaggaagg cgccgtccgt
     7201 gcccggatag acgacctcac cgaccgcgtc ggagcccccg cgggcggtga gctcaccgaa
     7261 ggccgagtcg ttcatcgtca ggaagccgca cgagccctcg acgagctgac cgatcgcctg
     7321 atcccacgtc tgggtgtcgg cgtccgctga ggtgtatgcc agcaggtcgt cgagttcacc
     7381 gagggcgtcc ttgacctcgg tgccgttcca gtcaagcttg tcgtcctgga ttttggccca
     7441 tccgtccggt ccgagccggg cgagcaggat gttgtcgagg agctcggcgg tggtgaagcg
     7501 atccttggca ccgaggcaca gcgccggcac accggacgcc ttgaccttct cgaggtcggc
     7561 gcggaacgcg gcgggcgtgt agcccgccgc cggcgcggcg acgcccgctt tgtcgagcac
     7621 ggccttgttg aagaacaaca cattgctgcg gtgggcgccg gtgggcacgg catactgctt
     7681 gccgtcgatc gtcaccgact gaaggatcgt cggattgact tgcgccgcaa ggccaccggc
     7741 gccgaacacc gaactgacgt cccggatctg cgccgatttc tcgtatgccg tcgtcgcggc
     7801 gccggcgaag gtctgccaca cgtcgggcgg gtcgccggcg atgagtcgct tggcgagcgc
     7861 gaccctcgcg ctgctgccgg cgcccccctg gacgctcgcg ttggtgaggg tcacgccggc
     7921 attggccccg gcatacgcat cggtgaagac cttgaaggcc ttggcttccg acgccgaggt
     7981 ccaccacgtc atcacttcga gctggtcggt cgcggcagac gggtcggtct ctttcgggcc
     8041 ggtgcaggca ctcaggccgg cgaccgccac ggtgacggcg agcaaggcac gagtcacgtt
     8101 gcgcatgatc atcacgcttc cgaggcgagc tcggcgctgc cgccgggcgc tttgaggtcg
     8161 tacagggcga aggccgtcac gacagcgaaa cagcatgccg ggacgatgaa ggagacggcg
     8221 gccgaggtct tgtcgatgac gatcccctgc acgagcggca tgatcgcgcc gccgacgatc
     8281 gccatcacca ggccggccgc cccgaacttc gtggcgggcc ccaggccttg cagcgcaacc
     8341 ccgtagatcg tcgggaacat cagtgacaga cagaacgaga cggcaacgat ggcggcgacg
     8401 ccgaccatgt tgggcgagac cacggcgatg aggcagagaa cgatcgcgag cgtgccgagg
     8461 gcggcgagca ccttggtcgc gcggatcttg ccgatcaccc aggtcatgag gaagcgcgag
     8521 agcatgaaga cgatgaggct gacctggagc agatacccgc cgagttcgag actgccgttc
     8581 aatgcctgct ggacgtactg gaggatgtag gtccagcagc acacctgcgc tgcgacattg
     8641 aagaactgcg cgacgacgcc gaagcggtag cgacggcgtc cccacagggt cttgatgacg
     8701 ctctgctcgg tctcgccctc cgatgtcggg tggaactcct catggatcgg cggggacttc
     8761 tggatggcga tgaccagcca gatgacgagc agcaggaagc cgaggccgag gtagggcccc
     8821 atcaccgcgc cgagctgacc ggcccggatc gcctgttgct cggcgggagt cagggcgccg
     8881 acaccgacgg gggcctcgag tttcggcagg atcaacgtcg acgcgagcag gacgccgata
     8941 ttcgtgccca gcgggttgaa ggactgcgcg aagttgagcc ggcgggtggc ggtcgactcc
     9001 ggacccaggg cgatgacgta ggggttggcc gaggtctcga ggatggagca tccggccgcc
     9061 atcgcgaaga gcgcaacgag gaaggcctcg taggtcatga tcttgctcgc cggatagaag
     9121 gcgaaggctc cgatggcagc cagcgccaga ccggtcagga gtccgacctt gtaaccgaac
     9181 cgtttgttga tgaacgcggc gggcaacgcg agcaggaagt aggcgccgaa gtaggccagt
     9241 tgcaccaggg atgcctggaa ggtgctcatc tcgaagatct gtttgaaccc cgcgaccatg
     9301 ggagtggtca gatccgccgc gatgccccat gccgtgaagc acgcgacgag cacgacgaaa
     9361 agaaccagca tccccggtcc gacgatcggg tccttggtgg cgcgcgcgtc cgatgcttcc
     9421 ttcaccggtg tctggctcaa ggcaaccccc ttgtcgcgaa tctgaccgaa gaatcttcag
     9481 cacacgcgcc cgcgcgcaca atgcgcaggt cggcccgctg ttcgtgaacc tacgcccgcc
     9541 gagacggcgc ggcaaccgga gagcgggagc ccgctggacg ggctctaggc aacatgtctg
     9601 gtgggtgaca acttggtacg gcaacttgtt tcacttactc acgagtcacc tagcaggcat
     9661 acctttgagt tgaccgttaa acaacggtcg aatgaccggc cgggtgatcc tcggccggga
     9721 tcggaggaaa aaccacacat ggcggccctc agtccgtgac ccccgacgca cgcggggtcg
     9781 atctcccctc ccgcgaggtc accttccaag aagacatcgt ccgtacgtcg gtgttgcgca
     9841 aactcctcgc ctgctgctcg ccctgatgta cctggccgag ttcccgcagg gtcagcctgc
     9901 tgacttccac gccaccaacc ccatcactga ctc
//
LOCUS       DAOPJG010000130         9647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7402_length_9647_cov_7.892515, whole genome shotgun sequence.
ACCESSION   DAOPJG010000130 DAOPJG010000000
VERSION     DAOPJG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7402_length_9647_cov_7.892515"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKU75_11570"
     CDS             <1..717
                     /locus_tag="PKU75_11570"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPF81564.1"
                     /translation="WEPPSRMVISWHPGRDTGEQSEVEVRFEPDAAGTRVIIEHRGWE
                     TFGADAMRRRRGYVGPSAWGYVLDHFADVAEPRDQAPDLGGLAAAYDAFFAEAERGGF
                     GPPPADGEWDADRTLAHVALSDLATIAVSQAIIHQEPARFANVDCQTPDHLAAWIVRC
                     GNTTGLIAEGRAVAQQVMAVLARLSPQQLAQAVPCYLLHDGQVLVDEPRPWGTIAIHG
                     QAGMHLPAHTGQLSNLRPMT"
     gene            781..987
                     /locus_tag="PKU75_11575"
     CDS             781..987
                     /locus_tag="PKU75_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HPF81565.1"
                     /translation="MTKRLIDLDDDLLEAARDALGTSGVSDTVRAALRMAADNAARAR
                     EVEWLRAGALEPLADAEARAAVWR"
     gene            978..1403
                     /locus_tag="PKU75_11580"
     CDS             978..1403
                     /locus_tag="PKU75_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPF81566.1"
                     /translation="MALIARYLIDTSAAARMRHPEVAERLVPLIEGALVATCPTLDAE
                     ALFVARDAPEYAAIRENRQGAYEHLPLENAHWELAFDAQQALAVTGRHRAVGMADLLT
                     AVLAQEHRLTVIHYDADFEIAGEVLHFAQAWVRPRGSLS"
     gene            complement(1437..2720)
                     /locus_tag="PKU75_11585"
     CDS             complement(1437..2720)
                     /locus_tag="PKU75_11585"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPF81567.1"
                     /translation="MSEAFDAVDVIIAKRDKKELSDEQIRWVIDAYTRGAVAEEQMSA
                     LNMAVLLNGMTRREIATWTGAMIASGERMDFSSLSRPTTDKHSTGGVGDKVTLPLAPL
                     VAVFGVAVPQLSGRGLGHTGGTLDKLESVPGWQAALSNEEMMAQLEDVGAVICAAGSG
                     LAPADKKLYALRDVTGTVEAIPLIASSIMSKKIAEGTGALVLDVKVGSGAFMKNLDDA
                     RELARTMVDLGTDAGVKTVAVLTDMATPLGLTAGNALEVRESVEVLAGGGPADVVELT
                     VKLAEEMLEAAGRGGEDVRAALQDGRALDVWKRMIRAQGGDPDAPLPVAKEREQVVAE
                     RDGFLTTMDAYAVGVAAWRLGAGRARKEDPVQAGAGVEIHCKPGDQVRKGDPVLTLHT
                     DTPERFGRAKEALSGGIEIGDAAPEKTSIVLDRID"
     gene            complement(2792..>2943)
                     /locus_tag="PKU75_11590"
     CDS             complement(2792..>2943)
                     /locus_tag="PKU75_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPF81568.1"
                     /translation="LMPCGRCRQLLWENGGPACLLMTPEGVLPMSQILPQAFGPDDLA
                     TRSGH"
     assembly_gap    2944..3043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3044..4284)
                     /locus_tag="PKU75_11595"
     CDS             complement(<3044..4284)
                     /locus_tag="PKU75_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF81569.1"
                     /translation="MSTVVTPTPTPTPGAAPARGSFLANPWARWGMIVLGAIILMSAL
                     RVITGANDLDSSGAIAAAFGLAVPIALAGLGGLWCERAGVVNIGLEGMMILGTWSSAF
                     CAYHWGPWVGILGGALGGLVGGLIHAIATVYFGVDHIVSGVALNIIGLGVAKYLASRF
                     FTGLPGGGPTQSPPLEYAPSLSLPGIGDPLLTLERKHIFFVSDLAAIVRGLTTNVSVL
                     TLITLGLFIATWYVLWRTAFGLRLRSVGESPAAAESLGVNVYKYKFIAVLASGSLAGL
                     GGAFLTLVASNLFRDGQTGGRGYIGLAAMIFGNWRPGGLLAGSALFGYTDAIRLRAGG
                     QSVHAVLLLVAVLLLLIGLWQLFRRGRVVQGVLALVVAGLTAWWYFAADTVPNELAGT
                     LPYVTTLLVLAFAAQRLRMPAA"
     gene            complement(4281..5399)
                     /locus_tag="PKU75_11600"
     CDS             complement(4281..5399)
                     /locus_tag="PKU75_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF81570.1"
                     /translation="MTNANLRRALLSIAAPILALFVAFLLTSLVLMALGDPVKDVWIT
                     MLSKPKPRQITNIVNGAIVLYLSAIAVAVGFKMNLFNIGVDGQYRVGSFAAALFAGQA
                     WLPGVLNILVAIVVAMVAGGFWASIAAYLKVKRGVSEVISTIMLNAIATGVVSWLLLK
                     VAVRTAGSNAIGTKTIPESSQLEGWKLIPGASNRVYSLLLLAILVGLLYWFVLGKTRF
                     GFDLRATGSSETAAVASGVKVKRMVMTSMIASGALAGLIGMPLLFGQDHSYGTTFQAG
                     LGFAGIGIALLGRNHPVGIAFAALLWSYLDTQANGLQIRAGVAPELVYIIQGIILFAV
                     VIAYEGTRRLDIRLEQQRVAGELAAQRAAVRDQEGAVA"
     gene            complement(5396..6961)
                     /locus_tag="PKU75_11605"
     CDS             complement(5396..6961)
                     /locus_tag="PKU75_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81571.1"
                     /translation="MANKDISFAVRRGTVHALVGENGAGKSTLMKILYGVQRQDEGTI
                     KVNGSEVTLHSPSDAIDAGIGMVFQHFMLADNLTVLENVVLGAEKLHGIGGGARAEIR
                     RIAEDFGLDVDPDDLVADLGVGDRQRVEILKVLYRGAKILILDEPTAVLVPQEVDELF
                     DNLRELKAEGLTVIFISHKLDEVLSVADDITVIRRGTTVASVDPSEVNARQLAELMVG
                     SQLPTPETSESTVTDRVVLSMRDITLAGGEGRDLLHDIDLDIHAGEIVGVAGVEGNGQ
                     AELVEVIMGMREPTAGTVHLEGTDISDWEVREIREAGVGYIPEDRHRHGLLLDAPLWE
                     NRILGHQTERPNVKGGLIDARGAKSDTERIVREYDVRTPSIFVTAGSLSGGNQQKLIV
                     GREMSHASKVLVASHPTRGVDVGAQSAIWDLMRQARHEGLGVLLISADLEELIGLSDT
                     IRVILRGRLTGTFDPDTVTSQDLGAAMTGADGSHGDAPAPADAGGDAAYPDSLTGPAL
                     APSPAADDIEDPR"
     gene            complement(7162..8127)
                     /locus_tag="PKU75_11610"
     CDS             complement(7162..8127)
                     /locus_tag="PKU75_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPF81572.1"
                     /translation="MSSSAPAGNSDLKVGMAYDVGGRGDQSFNDAAAAGMDKAKAELG
                     IETKDATAVNGENEAAREERLNQLVDAGYTNIIAVGFAYAVSVGKVAKENPDVHFAII
                     DDASDASKGDNVAQLTFAEQEGSFLVGAAAALTTKEDHIGFVGGVNVPLIVKFEAGYE
                     AGAKAVNPDIKIDSTYLTQPPDMSGFGDPAKGKTAAEGMFQGGADVVYHAAGGSGAGV
                     FTAAKAAKGWAIGVDSDQAKTAEAGVQDVILTSMLKKVDVAVYDYLSKAAAGSPVSGN
                     VIYDLKADGVGYSTTGDHLSADIIAKLDDYKAKIISGEITVPDTK"
     gene            8126..8296
                     /locus_tag="PKU75_11615"
     CDS             8126..8296
                     /locus_tag="PKU75_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81573.1"
                     /translation="MLSLPDADALSDALPLAEALVAPALALLADDSLLVPEPHAARAR
                     LLAETTAATFIS"
     gene            complement(8412..9623)
                     /locus_tag="PKU75_11620"
     CDS             complement(8412..9623)
                     /locus_tag="PKU75_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPF81574.1"
                     /translation="MTPDSFAAVRGAIATRQAQLVTIRRDIHRHPEISWQEQRTTALV
                     CDLLEEGGLKPTRLSKSGLVVDIGAEDAAYRVALRADIDALPVRERTGLDFASVSPGV
                     CHACGHDVHTTALVGAGLALAGQDEALRAADAAVRLVFQPAEEAIPGGAELVVGEGHL
                     EGVDAAYALHCDPSLDVGKVGLREGPITAAADRIDVKLTGRGGHTSRPHLTEDLTYAL
                     AKVVTEVPALLSRRVDPRAGALLVWGQMHAGAAGNVIPSSGEATGTLRILDAEVWHQI
                     GPIVKELVESVAKPYGVGVEVVHSPGVPPVVNDATAIADLRAGAVQMGLDPVATRQSM
                     GGEDFAWFLHHAPGAMARLGTRAPGGRTYDLHQGDLIVDEAAISAGAMLLASTALAAI
                     GRYAANHATSS"
BASE COUNT         1538 a         3290 c         3248 g         1471 t
ORIGIN      
        1 tgggaaccgc cgagcagaat ggtgatctcc tggcatccgg gccgggacac cggcgaacag
       61 tccgaggtcg aggtgaggtt cgaacccgac gcggccggca cccgagtgat catcgaacac
      121 cgcggctggg agaccttcgg ggcagacgcc atgcggcgtc gtcgcggata cgtcggcccc
      181 tcggcgtggg gctatgtcct cgaccacttc gccgatgtcg ccgagccccg cgatcaggcg
      241 ccggatcttg gcggcctcgc cgcggcatac gacgccttct tcgccgaggc ggagcggggc
      301 ggcttcgggc cgccaccggc tgacggcgaa tgggatgccg accggacgct cgctcacgtc
      361 gccttgtccg acctggccac gatcgcggtg agccaagcca tcatccacca ggagccggcc
      421 cggttcgcca atgtggactg ccagacgccg gaccaccttg cagcctggat cgtccgctgc
      481 ggcaacacaa ctggcctgat cgccgaaggt cgggccgtgg cacagcaggt catggctgta
      541 ctcgcgcgtc tctcccccca gcagctggcg caggcggtcc cgtgctatct gctgcacgac
      601 ggccaggtcc tcgtggacga gccgcgcccg tggggcacca tcgccatcca cggacaagcc
      661 ggcatgcacc tacccgcaca caccggccag ctgagcaacc tgcggcccat gacctgacgc
      721 cattctcgtc accgttgcta tatatactcc tgatggtgac gtatatatcg gaggcgaacc
      781 gtgaccaaac gactcatcga ccttgacgac gatctcctcg aggcagcgcg cgacgccctc
      841 gggacgtccg gcgtctccga cacggtgcgt gcggcacttc gtatggcagc ggataacgcg
      901 gcccgtgctc gtgaggtcga gtggttgcgg gccggagcac tcgagcccct cgccgatgcg
      961 gaggcccggg ctgcggtatg gcgctgatag cgcgttacct catcgacacc agcgcagcgg
     1021 cgcggatgcg gcaccccgag gtggccgagc gcctggtccc actcatcgaa ggtgcgctcg
     1081 tcgctacctg cccgacgctc gacgccgagg ctttgttcgt ggcccgggat gctccggagt
     1141 atgcggcgat ccgggagaac cggcaggggg cttacgagca cttgccgctc gagaacgcgc
     1201 actgggaact cgcgttcgac gcacagcagg cgcttgccgt tactggcaga catcgcgccg
     1261 tgggtatggc cgacttgctc accgctgtcc tcgcacagga acaccggctg acagtgattc
     1321 actacgacgc cgacttcgag atcgcggggg aggttctcca cttcgcgcag gcgtgggtcc
     1381 ggcctcgggg ctcgctcagt tgacctgaat ccctgggatt tgaggacgac ctggtgtcag
     1441 tcgatccggt cgaggacgat tgaagtcttc tccggggccg catcgccgat ctcgatgcca
     1501 ccggacaagg cttccttggc gcggccgaat cgctcggggg tgtcggtatg cagtgtcagg
     1561 acagggtcac ccttgcgcac ctggtcccct ggcttgcaat ggatttcgac gccggcgccg
     1621 gcctgcacgg gatcttcctt gcgcgcccgg ccggccccca ggcgccaggc ggcgacgccc
     1681 acggcatacg catccatcgt cgtgaggaat ccatcgcgct cggcgaccac ctgctcgcgc
     1741 tccttggcca ccggcagcgg cgcgtccggg tcgccgccct gggcgcggat catgcgcttc
     1801 cagacatcga gggcacgccc atcctgcagc gccgcccgca cgtcttcgcc accgcgtccc
     1861 gccgcctcca gcatctcctc ggcgagtttg accgtcagct ccacgacgtc ggccggaccg
     1921 ccgccggcca gcacctccac cgactcgcgc acctccaagg cgttgccggc cgtcaacccc
     1981 aacggggtag ccatatccgt cagcaccgca acggttttca ccccagcgtc ggtgccgagg
     2041 tcgaccatgg tgcgggcgag ttcgcgggcg tcgtcgaggt tcttcatgaa ggcgccagac
     2101 ccgaccttga cgtcgagcac cagcgcgccc gtgccctcgg cgatcttctt ggacatgatc
     2161 gaactcgcga tgagcgggat cgcctcgaca gtgccggtga cgtcacgcag cgcatacagc
     2221 ttcttgtccg cgggagcgag gccggagccc gctgcgcaga tgaccgcgcc gacgtcctcg
     2281 agttgcgcca tcatctcctc attcgaaaga gcggcctgcc agcccgggac cgactcgagc
     2341 ttgtcgagcg tgccgcccgt gtgcccgagc ccgcgccccg acaactgcgg cacggccacg
     2401 ccgaagactg ccaccagggg cgccaagggc aaggtgacct tgtcgcccac accaccggtc
     2461 gagtgtttgt cggtcgtggg ccgagagagg gacgagaagt ccatccgctc ccccgaggcg
     2521 atcatcgcgc cggtccacgt cgcgatctcg cgccgcgtca taccgttgag cagcaccgcc
     2581 atgttgagcg cggacatctg ctcctccgcc acggcgccac gcgtgtaggc gtcgatgacc
     2641 cagcggatct gctcgtcact caactccttt ttgtcgcgct tggcgatgat gacgtcgacg
     2701 gcatcgaagg cttcgctcat gcgggcaaac taccagcggc gcgcccgcgg tccccgcgac
     2761 cggcagagtg acatgacgac gggggcgggg gtcagtggcc ggaacgggtc gcgaggtcgt
     2821 cggggccgaa ggcttgcgga aggatctgcg acatcggcag gactccctcc ggggtcatca
     2881 ggagacaggc gggaccaccg ttctcccaca acagttgccg gcaccgcccg cagggcatga
     2941 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcggccg gcatccgcaa
     3061 gcgctgggcg gcgaaggcaa gcacgaggag ggtggtgaca tagggcaggg tgccggcgag
     3121 ttcgttgggg acggtgtcgg ccgcgaagta ccaccaggcg gtcaggccgg cgacgacgag
     3181 cgccaacacg ccctggacga cccggccgcg ccggaacagc tgccagaggc cgatcagcag
     3241 caacagcacc gccacgagga gaagcaccgc gtgcaccgac tgtccaccgg cacgcagccg
     3301 aatggcgtcg gtgtagccga acagcgccga gcccgcgagc agcccaccgg ggcgccagtt
     3361 gccgaagatc atcgcggcca gaccgatata gccgcggcca ccggtctgcc cgtcgcggaa
     3421 gaggttggag gccaccaagg tcaagaaggc accgccgaga ccggcgagcg agccggacgc
     3481 cagcaccgcg atgaacttgt acttgtagac gttgacaccg agcgattcag cggcggccgg
     3541 cgactccccg accgagcgca gccgcaggcc gaaagccgtg cgccacaaca cataccaggt
     3601 cgcgatgaat agcccgaggg tgatcagcgt cagcaccgac acattcgtcg tcaaccctcg
     3661 cacgatggcg gcgaggtcgg agacgaagaa aatgtgttta cgctccagcg tcaacagcgg
     3721 atcgccgatc ccgggcaagg acaaggaggg cgcatactcc agcggcggcg actgggtcgg
     3781 gccaccgccg ggcaggccgg tgaagaagcg cgacgcgagg tacttcgcca cccccaggcc
     3841 gatgatgttc aacgccacac cggagacgat gtggtcgacc ccgaagtaga cggtcgcgat
     3901 ggcgtggatc aggccgccga ccagaccgcc gagcgcgccg ccgaggatgc cgacccacgg
     3961 cccccagtga taggcgcaga aggccgacga ccaggtgccg aggatcatca tcccctcgag
     4021 gccgatgttg accacaccgg cccgctcgca ccacaggccg ccgagaccgg cgagggcgat
     4081 gggcacggcg agcccgaacg ctgccgcgat ggcgccggag gagtccaggt cgttggcgcc
     4141 ggtgatgacg cgcagcgcgg acatcaggat gatcgcgccg agcacgatca tgccccagcg
     4201 cgcccacggg ttggcgagga acgacccgcg ggcaggggcc gccccgggcg tcggcgtcgg
     4261 cgtgggcgtg acgacggtgc tcatgccacc gctccttcct gatcgcggac cgcggctcgc
     4321 tgagcggcca actcccctgc gacacgctgc tgttcgagcc ggatgtcgag gcgacgcgtg
     4381 ccctcgtagg cgatgacgac ggcgaagagg atgatgccct ggatgatgta caccagctcc
     4441 ggagcgacgc ccgcgcggat ctgcaggccg ttggcctgcg tgtcgagata gctccacagc
     4501 agcgcggcga aggcgatgcc gaccgggtgg ttgcgcccga gcagcgcgat gccgatgccg
     4561 gcgaagccga gacccgcctg gaaggtcgtg ccatagctgt ggtcctgccc gaagagcagc
     4621 ggcataccga tcagacccgc gagcgcaccg gaggcgatca tcgacgtcat caccatccgc
     4681 ttgaccttca cgccactcgc gaccgccgct gtctcggacg aaccggtggc gcgcaggtcg
     4741 aacccgaacc gggtcttgcc gagcacgaac cagtagagga gcccgacgag gatcgccagc
     4801 agcagcagac tgtagacccg gttgctcgcg ccggggatga gcttccagcc ctccagttgt
     4861 gaggattcgg ggatcgtctt ggtcccgatc gcgttggagc cggcggtccg caccgccacc
     4921 ttcagcagca gccatgacac caccccggtg gcgatggcgt tgagcatgat cgtcgagatg
     4981 acttcggaga caccgcgttt gactttcagg tatgccgcga tgctcgccca gaacccgccc
     5041 gccaccatcg cgacgacgat cgccacgagg atgttgagaa cgcccggcag ccacgcctgg
     5101 ccggcgaaga gcgcggccgc gaacgacccg acccggtatt gcccgtcgac gccgatattg
     5161 aacaggttca tcttgaaccc cacggcaacc gcgatcgccg acagatagag cacgatcgcg
     5221 ccgttgacga tgttcgtgat ctgccgcggc ttcggcttgc tcagcatcgt gatccacaca
     5281 tccttgaccg ggtcgccgag cgccatgagc accagcgagg tcaggaggaa ggcgacgaag
     5341 agagccagga tcggggccgc gatcgacagg agggcccggc gcaggttggc gttggtcatc
     5401 gggggtcctc gatgtcgtct gcggccggtg acggggcaag cgccggtccg gtcaaggagt
     5461 cagggtatgc cgcgtcgccg ccggcgtcgg ccggcgcagg tgcgtcgccg tgcgagccgt
     5521 cggcgccggt catcgccgcg ccgagatcct ggctggtgac ggtgtcgggg tcgaaggtgc
     5581 cggtcaaccg gccgcgcagg atgacccgga tggtgtcgga caggccgatc aactcctcga
     5641 ggtcggcact gatgagcagc accccgagcc cttcgtgccg ggcctgccgc atcaggtccc
     5701 agatcgccga ctgcgctccg acatcgacgc cgcgggtcgg gtgggaggcg accagaacct
     5761 ttgaggcgtg cgacatttcg cggccgacga tgagcttctg ctgattgccg cccgacaacg
     5821 aaccggccgt gacgaagatc gagggggtgc gcacgtcata ctcccgcacg atgcgctcgg
     5881 tgtccgactt ggcgccgcgg gcgtcgatca gcccgccctt gacgttgggc cgttcggtct
     5941 ggtgaccgag gatccggttc tcccacaagg gggcatccag cagcagcccg tggcggtggc
     6001 ggtcctcggg gatgtatccc actccggcct cgcggatctc gcgcacctcc cagtcggaga
     6061 tgtcggtgcc ttcgagatgc accgtcccgg ccgtgggctc gcgcatcccc atgatgacct
     6121 cgaccagctc ggcctgcccg ttgccctcga cgcccgcgac cccgacgatc tcgcccgcgt
     6181 ggatgtcaag gtcgatgtcg tgcaacaggt ctcggccttc gccgccggcg agggtgatgt
     6241 cccgcatcga cagcacgacc cggtcggtga ccgtcgactc gctcgtctcg ggagtgggca
     6301 gctgcgagcc gaccatcagc tccgcgagct gacgtgcgtt gacctcggac ggatcgaccg
     6361 aggcgaccgt cgtgccgcgc cggatgacag tgatgtcgtc ggcgaccgac aacacctcgt
     6421 cgagcttgtg cgagatgaag atgaccgtca ggccctcggc cttcagttcg cgcaggttgt
     6481 cgaagagttc gtcgacctcc tgcggcacga gcaccgcggt gggctcgtcg aggatgagga
     6541 tcttggcgcc gcgatagagg accttgagga tctcgacccg ttgccggtct cccacaccga
     6601 gatccgcgac aaggtcgtcc gggtcgacat cgagaccgaa gtcctctgcg atccggcgga
     6661 tctccgcgcg cgctcctccg ccgatgccgt gcagcttctc cgcgccgagg acgacgttct
     6721 cgaggacggt gagattgtcg gcgagcatga aatgctggaa aaccatgccg atgccggcgt
     6781 cgatcgcgtc ggacggcgaa tgcagggtga cctcggaccc gttgaccttg atcgtgccct
     6841 cgtcctggcg ctgcaccccg tagaggatct tcatcagcgt cgacttgccg gcgccgttct
     6901 cccccaccag tgcgtgcacg gtgccgcggc ggacggcgaa ggagatgtcc ttgttggcca
     6961 cgacacccgg gaagcgcttc gtgatcccgg tgacttcgac ggcgagatcg cctgtcgggg
     7021 ggctggctac tgcggcgatg acgagacctc catgtcactc gttggccggt atgaccggcc
     7081 accctgccgg gagcgacaag caggggctgg gtgaacgcta cacgtccacc cagcccctgc
     7141 cttgttcgcc gacccggagg gttacttcgt gtccggaacc gtgatctcgc cggagatgat
     7201 cttggccttg tagtcgtcga gcttggcgat gatgtccgcg ctcaggtgat cgccggtggt
     7261 ggagtagccc accccgtcgg ccttgaggtc gtagatgaca ttgccggaca ccgggctgcc
     7321 cgcagccgcc ttgctcaggt agtcgtagac cgcgacgtcg accttcttga gcatcgaggt
     7381 cagaatgacg tcctggacgc cggcctcggc ggtcttcgcc tggtcggagt cgacgccgat
     7441 ggcccagccc ttggcggcct tggcggcggt gaagacaccc gcgcccgacc cgcccgcggc
     7501 gtggtagacg acgtccgcgc caccttggaa catgccctcg gcagcggtct tgcccttggc
     7561 cgggtcaccg aagccgctca tgtccggcgg ctgggtgaga taggtggaat cgatcttgat
     7621 gtcggggttg acggccttcg cgcccgcctc gtagcccgcc tcgaacttca cgatcagcgg
     7681 cacgttgacg ccaccgacga agccgatgtg gtcctctttg gtggtcagcg cggccgcagc
     7741 accgacgagg aaggacccct cctgctcggc gaaggtcagc tgcgcgacgt tgtcgccctt
     7801 cgaggcgtcg gaggcgtcgt cgatgatggc gaagtggacg tccgggttct ctttggccac
     7861 cttgccgacc gacacggcat acgcgaaccc gaccgcgatg atgttggtgt agcccgcgtc
     7921 cacgagctgg ttgaggcgct cctcgcgggc ggcctcgttc tcgccgttga cggcggtcgc
     7981 gtccttggtc tcgatgccga gttcggcctt ggccttgtcc atgcccgcag cggcggcgtc
     8041 gttgaaggac tggtcgccac gaccgcccac gtcatacgcc ataccgacct tgaggtcgga
     8101 gttgcccgcg ggggcggagg acgacatgct ctcgctgccc gacgccgacg cgctctcgga
     8161 cgcgctgccg ctcgccgagg cgctcgtggc gcccgcactg gcgctgctcg ccgacgattc
     8221 cttgctcgtg cccgagccac acgcggccag ggccaggctg ctggccgaga cgaccgcggc
     8281 caccttcatc agttgacgca aggtgctctc ctcatttctt ccagtcgagg gcacgatcgc
     8341 gcccactgaa gacgaaggat aagccgcgcc gcaggaagtc cgtcgtcaca cccgtcccaa
     8401 tgcgggaagt gttacgaact cgtggcgtga tttgcggcat accgcccgat cgcggcgagg
     8461 gcagtggagg ccagcagcat cgcgccggcg gagatcgccg cctcgtcgac gatcaggtca
     8521 ccctggtgca ggtcgtatgt ccgaccgccc ggcgctcgcg tgcccagacg cgccatcgcg
     8581 ccgggagcgt ggtggaggaa ccacgcgaag tcctcgcccc ccatcgactg ccgggtggcg
     8641 accgggtcca ggcccatctg caccgccccg gcccgcaggt ccgcgatcgc cgtcgcgtcg
     8701 ttgacgaccg gcgggacacc gggcgagtga acgacctcga cgcccacgcc atacggcttg
     8761 gcaacggact cgaccagctc cttgacgatc ggcccgatct ggtgccacac ctcggcgtcg
     8821 aggatccgca gcgtgcccgt cgcctcaccg gaggagggga tgacgttgcc ggccgcgccg
     8881 gcgtgcatct gcccccacac cagtagcgcg cccgcccgcg ggtcgacccg gcgcgacagc
     8941 agcgcgggca cctcggtgac caccttcgcg agggcgtagg tgaggtcctc ggtcaggtgc
     9001 ggccgggagg tatggccgcc gcggccggtc agcttgacgt cgatgcggtc ggctgctgcc
     9061 gtgatcggcc cttcgcgcag gcccaccttg ccgacgtcga gactggggtc gcagtgcagg
     9121 gcgtatgccg cgtcgacacc ctccaggtgc ccctcgccga cgacgagttc ggcgccgccc
     9181 gggatggcct cctccgccgg ctggaagacc aggcgcaccg cagcgtcggc tgcgcgcagc
     9241 gcctcgtcct gtcccgcgag ggcgagcccc gcgccgacga gggcggtcgt atgcacgtcg
     9301 tgcccgcacg cgtggcagac gccggggctg acggaggcga agtcgagtcc ggtgcgctcg
     9361 cgcaccggga gggcgtcgat gtcggcgcgc agcgccaccc ggtatgccgc gtcctccgcc
     9421 ccgatgtcga cgaccagtcc gctcttgctc aagcgggtgg gcttcagccc gccctcctcg
     9481 agcaggtcgc acaccagcgc ggtggtgcgc tgctcctgcc agctgatctc ggggtggcgg
     9541 tggatgtcgc gccggatggt caccaattgc gcctggcggg tggcgatggc gccgcggacg
     9601 gcggcgaagg agtcgggtgt catgtcgacg gacaacgtta ccggccc
//
LOCUS       DAOPJG010000131         9594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7464_length_9594_cov_8.347101, whole genome shotgun sequence.
ACCESSION   DAOPJG010000131 DAOPJG010000000
VERSION     DAOPJG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7464_length_9594_cov_8.347101"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..959
                     /locus_tag="PKU75_11625"
     CDS             81..959
                     /locus_tag="PKU75_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81575.1"
                     /translation="MTALRRFAFGPVSLARIAWLRLVIYLYVVWDVLMVVTDPIAHGQ
                     VPVELYHPVWAREWLGFPAPSPVYVQVLRVVILASALVAASGRLPRVAGAVCAVGMFD
                     WITNAFSYSKIDHDHYALMIALLVLPTVRRARFGDVGVVSEAAGWAVRMIQVSAVSIY
                     VLSAWAKMRFGTWGWANGSTLIWALTRRPNGLAPWIGSHPTLTHVLQWIMLVAEFLSP
                     ALLWLRGRALAAGLLFFLSFHATTYYLLRIHFLPLVVCLTAFMPMERIPAWWARRRRV
                     DSAHPPAVPAAPWSGR"
     gene            complement(859..1458)
                     /locus_tag="PKU75_11630"
     CDS             complement(859..1458)
                     /locus_tag="PKU75_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPF81576.1"
                     /translation="MAEADIGAPLPEEVLVEAAGEFAGSADGFERLWTPHRMAYIRGE
                     RPAEDGGEGCPFCAGPDRSDQDGLIVHRGVECFVVMNLFPYNPGHVLVCPYRHVSLYV
                     DLTESETVEFTALTKAAVAALESASAPHGFNIGMNQGAVAGAGVAAHLHQHVVPRWGG
                     DANFFPIIARTKALPALLEDVRSQLAAAWPTTPGSAPSA"
     gene            1581..2930
                     /locus_tag="PKU75_11635"
     CDS             1581..2930
                     /locus_tag="PKU75_11635"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HPF81577.1"
                     /translation="MPQLPQDFLFGVATAAYQIEGAVTEDGRGRSVWDDFCDRPGAIV
                     DGSTGASACDSYHLYKEDVDLMAGLGIDAYRFSISWPRIQPDGFGAVNQAGLDYYDRL
                     VDALLARGIRPAATLFHWDLPSPLEAEGGWLQRETAEAFADYARIVAERLADRVAMWT
                     PVNEPNIVTSLGYSTGMHAPGRTLGLYALPAAHHLLLGHGLAVGALRAAGAKSVGCAQ
                     NHTVVWPTSQEESDVAAADLFDTIFNRMYAEPILHGTYPDGIAQMMPGPVEHDVKIIG
                     APLDFYGFNYYNPTLVGSPHAGERIGAEIAVDLPFSRHEIQGYPMTDFGWPVVPEGLL
                     AVMRQLRERYGDRLPPLFVTENGCAYNTGPDDKGVVMDTERVTFYDGHLAAVSQAIAE
                     GIDVRGYFAWSLMDNFEWAEGFTKRFGLVYTDFETQQRIPKLSYDWYADLIRRTKAG"
     gene            3007..3678
                     /locus_tag="PKU75_11640"
     CDS             3007..3678
                     /locus_tag="PKU75_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF81578.1"
                     /translation="MKATTREAKRPDGRSTRWESHRVIRRAELLQSSIAAIRAQGPNL
                     GMDEFAAAAGTSKTVLYRHFTDRAGLHQAVAEAIEAMIAESVGQALLLGDARPTPRSV
                     VAAAVRAYVALVESEPNLYDFLFRAPLLESPAGRPDLAQAKVTLDLAGRVRVLLDQNA
                     TTTYPNGTTQLWAEALLGLVRTGVDVWKRGDRALSAEQVTELLTELAWHGASTAFHTK
                     GPRPV"
     gene            complement(3849..4067)
                     /locus_tag="PKU75_11645"
                     /pseudo
     CDS             complement(3849..4067)
                     /locus_tag="PKU75_11645"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
     gene            complement(4148..4221)
                     /locus_tag="PKU75_11650"
     tRNA            complement(4148..4221)
                     /locus_tag="PKU75_11650"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4185..4187),aa:Val,seq:cac)
     gene            complement(4284..4748)
                     /locus_tag="PKU75_11655"
     CDS             complement(4284..4748)
                     /locus_tag="PKU75_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02611 family protein"
                     /protein_id="HPF81579.1"
                     /translation="MTQESLDRNEIARHDGNIFLDKNDDDWEWRRKLRANPATAKLYR
                     LGVGLLGLIIVAGGLALVPLPGPGWLIVFAGLAVWASEFEWAQALLDWVRDKVRAWNV
                     WVKGQPLWVQGLAALVTLAAVLTFFWVLFRLSGVPSFMPDAAEDWLHTFAGL"
     gene            complement(4778..5626)
                     /locus_tag="PKU75_11660"
     CDS             complement(4778..5626)
                     /locus_tag="PKU75_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPF81580.1"
                     /translation="MSTVIRAWVNGALHDPAAPAISAVDHGITVGDGIFETCKILDGR
                     VFALSRHKARMARSANGLGLADIDFDRIDEGIAAVLGSGGPIAFGRLRFTVTAGRGPL
                     GSDREDSPLTYVVTAGPQAPPHPPGTLAVTPWTRNERSAVAGLKTTSYAENVVALAFA
                     HERGASEAIFANTRGELCEGTATNIFVVVAGRLHTPPLSSGCLAGITRELTIEWCREA
                     GMEVFEDAMPLGVLRTCEEVFVTSSLRDVYPIERIDDHRLAAPGRVTRTAMAIFAERS
                     AAEVDP"
     gene            complement(5623..6639)
                     /locus_tag="PKU75_11665"
     CDS             complement(5623..6639)
                     /locus_tag="PKU75_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPF81581.1"
                     /translation="MAWARFGAMTAYEPLAVSDDIDELTEGFWAVVVDFESTLTAVRF
                     AHRGRRTMTRPPGYRGWQPLDGTWRTSMDRAAYTAGVREIRERIAAGTVYQVNLCRVL
                     SHDLPADAYLPALARLLRKGNPAPYEALIHAPEFGVDIVCASPERYLRRDGERLVSSP
                     IKGTSRTPEEMLPKDYAENVMIADLVRNDLQQVCRVGTVAVEDLCAMENHPGLVHLVT
                     NVAGRLLPDVSWRDILDATFPPGSVSGAPKSSAVATIADLEPVVRGPYCGAIGWIDAD
                     TGEAELAVGIRTFWASEEQGRRVLHFGAGAGITWASDPEAEWEETQLKARRLIGLASG
                     AVAP"
     gene            6754..7215
                     /locus_tag="PKU75_11670"
     CDS             6754..7215
                     /locus_tag="PKU75_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81582.1"
                     /translation="MFETILGLPLHPLVVHAVVVLGPLAALLLVAYAAVPRWRVGLRI
                     PVLGLALIAAGSAFVADQAGEALMTVRGEPGFDHAEKGELAFISLLVLFLATIAVTVI
                     AKPLPAATNIVLPLVIAVAAAGFALFAVTQAGHSGAQSVWQGTFSLGVIGR"
     gene            7212..8303
                     /locus_tag="PKU75_11675"
     CDS             7212..8303
                     /locus_tag="PKU75_11675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPF81583.1"
                     /translation="MTRRRSPLAAALAVAVLAGCSATAGTGRTSAGASPAGANSVAPP
                     AKDVVLAFGGDIHFESYLAKLARDPAGLAALQKLWADADFRMANLETAITEGGTRVVK
                     AFNFRAPASALTTLAGAGMSAMSLANNHGVDYGLDGLADTLAARANSPFPIVGIGADE
                     ADAFAPKIVDVSGLKVALLGASQVNEETLAQFSAGPGKPGIASSMPPTRLVDAVARVR
                     AQADVVVVFMHWGMDYTDCPDQRSIDTSNALASAGADIVVGGHSHRINGAGWLGKSYI
                     AYGLGNFVWWRATEPDSRTGILRLTVDPALAKQPAAQRKTTVVVDATWQPLLIGRDGI
                     PATPPPADHDRLMDVWVAAGRCSGLTPTP"
     gene            8396..9364
                     /locus_tag="PKU75_11680"
     CDS             8396..9364
                     /locus_tag="PKU75_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPF81584.1"
                     /translation="MLAVAAVTQSTDDPLSGLHVGDIDEPPVPEDWVSIDVVTCALNH
                     HDLWSLKGVGLPGDRLPMILGCDAAGQAPDGAEVLVYPVISSPGFQGDVTNDPRRSLL
                     SEVYPGTLAERVRVPAGNLVPKPEGLSWEQAACLPTAWLTAYRMLFTNAAVHPGDTVL
                     VQGAGGGVATAAIQLAAAAGLRVWATSRDAAKRDLAKDLGATETFESGARLPDRVDAV
                     METVGAATWSHSVNSLRPGGTIVISGATSGDAPAKAELTKIFFKQLRVIGSTMGTRDE
                     LARLARFVADKGIEPQIDSVLPLAQARTGFERMAAGDVGGKIVFRV"
BASE COUNT         1403 a         3367 c         3352 g         1472 t
ORIGIN      
        1 gcccggacgc agaagatcat cgacctggct tcgtgggtgg ttgagaaccc ggcggacccc
       61 caagactggt cgggccggtc atgacggctc tgcggcgctt cgccttcggc ccggtcagcc
      121 tcgcgcggat cgcctggctg cggctggtga tctatctgta tgtcgtgtgg gacgtcctga
      181 tggtcgtcac ggacccgatc gcgcacgggc aggtcccggt ggagctctat cacccggtgt
      241 gggcgcgcga gtggctcggt tttccggcgc cgagcccggt gtatgtgcag gtcttgcggg
      301 tcgtcatcct ggcgtcggcg ctcgtggcgg ccagcggccg gctgccccgg gtcgccggcg
      361 cggtgtgtgc ggtcgggatg ttcgactgga tcacgaatgc gttctcctac agcaagatcg
      421 accacgacca ctatgcgttg atgatcgccc tgctggtgtt gccgacggtc cggcgggcgc
      481 gcttcggcga tgtcggggtg gtgtccgagg ccgccggctg ggcggtgcgg atgatccagg
      541 tctctgcggt gtcgatctac gtcctcagcg cgtgggccaa gatgcggttc ggcacgtggg
      601 ggtgggccaa cgggtcgacg ctgatctggg ccctgacccg ccggcccaac gggctggccc
      661 cgtggatcgg gtcgcacccg accctgacgc acgtgctgca gtggatcatg ctcgtcgcgg
      721 agttcctctc ccccgccctg ctctggctgc gcggccgagc cctggcggcg ggattgctgt
      781 tcttcctcag cttccacgcc acgacctact acttgctgcg catccacttc ctgccgctcg
      841 tggtgtgcct gacggcgttc atgccgatgg agcggatccc ggcgtggtgg gccaggcggc
      901 ggcgagttga ctccgcacat cctccagcag tgccggcagc gccttggtcc gggcgatgat
      961 cgggaagaag ttggcatcgc cgccccaccg gggcaccaca tgctggtgga ggtgggcagc
     1021 gacgcccgcc ccggcgacgg cgccctgatt catgccgata ttgaacccgt gcggagccga
     1081 agcggactcc agcgcggcga cggccgcctt cgtgagggcg gtgaactcga ccgtctcgct
     1141 ctcggtgagg tcgacataga gggacacgtg gcggtagggg cacacgagca cgtgcccggg
     1201 gttgtagggg aagaggttca tcacgacgaa gcactccacg ccccggtgga caatcagccc
     1261 gtcctggtcg gaccgatccg ggccggcgca gaaggggcag ccctcgccgc cgtcctcggc
     1321 cgggcgttcg cctcggatat aggccatccg gtgcggggtc cacagtcgct cgaacccgtc
     1381 cgcggacccg gcgaactcgc ccgccgcctc caccaggacc tcctcgggga gcggtgcccc
     1441 gatgtccgcc tcagccatgt ctcgatccta cgggcgagag gggggtgggc gaccggctcc
     1501 gcatgcgctt agggtgggaa cggcgccgcg tcccacggcg ccgttcccag gtcgctacgc
     1561 actgactcaa aggggccgac gtgccgcaac tcccccagga cttcctgttc ggtgtcgcca
     1621 ccgccgcata ccagatcgaa ggtgcggtga ccgaggacgg acgcggccgc agcgtctggg
     1681 acgacttctg cgaccggccc ggtgccatcg tcgacggcag taccggggcg agcgcctgcg
     1741 acagctacca cctctacaag gaggacgtcg acctgatggc gggcctcggg atcgacgcct
     1801 accggttctc gatctcctgg ccccggatcc agccggacgg gttcggcgcg gtcaaccagg
     1861 cgggcctgga ctactacgac cggctcgtcg acgcgttgct ggcccgcggc atacggcccg
     1921 cggcgaccct cttccactgg gacctgccct cgccgttgga ggccgaaggc ggttggctgc
     1981 agcgtgagac ggcggaggct ttcgccgact acgcccgcat cgtcgccgag cgcctcgccg
     2041 accgggtcgc catgtggacg ccggtcaacg agccgaatat cgtgaccagc cttggctatt
     2101 cgacggggat gcacgccccg ggacgcaccc tcgggctgta tgccctgccc gccgcccatc
     2161 acctgctgct cggccacggc ctcgcggtcg gcgccttgcg ggccgccggc gcgaagtcgg
     2221 tggggtgcgc ccagaaccac accgtggtct ggccgacctc gcaggaggag agcgatgtcg
     2281 cggccgcgga cctgttcgac acgatcttca accgcatgta tgccgagccg atcctgcacg
     2341 gcacctatcc cgacggcatc gcccagatga tgccggggcc ggtcgaacac gacgtgaaga
     2401 tcatcggcgc ccccctggac ttctacggct tcaactatta caacccgacc ctcgtcgggt
     2461 ccccgcacgc gggggagcgg atcggtgccg agatcgctgt cgacctgccg ttctcgcggc
     2521 acgagatcca gggttatccg atgaccgatt tcgggtggcc ggtcgtgccg gaggggctgc
     2581 tcgcggtgat gcgtcagttg cgtgagcggt atggcgatcg gctgccgccg ctcttcgtga
     2641 ccgaaaacgg ctgcgcctac aacaccggcc cggacgacaa gggagtcgtc atggacaccg
     2701 agcgggtcac cttctacgac gggcacctgg cggcggtgtc gcaggcgatc gcggagggta
     2761 tcgacgtccg cggctacttc gcctggtcgc tgatggataa cttcgagtgg gccgaaggct
     2821 tcaccaagcg attcggcctg gtctacaccg atttcgagac acagcaacgg attccgaagc
     2881 tgtcctacga ctggtatgcc gacctgatcc gccggaccaa ggcgggctga ccgcggcgga
     2941 cctgaccgcc ggggccgcgc cgtcgaccgg cccggaggcc agagccggga ctgttaccgt
     3001 gacccggtga aggcaaccac gagggaggcg aagcgcccgg acgggcggag tacgcgttgg
     3061 gagtcgcatc gcgtcatccg gcgggcggag ttgttgcagt cgtccatcgc cgcgatccgg
     3121 gcgcagggcc cgaatctggg tatggacgag ttcgcggcgg ctgcggggac gtccaagacc
     3181 gtgctctatc ggcatttcac cgatcgcgcc gggctgcacc aggctgtggc cgaggcgatc
     3241 gaggcgatga tcgccgagtc cgtcgggcag gccctgctcc tcggcgacgc ccggcccaca
     3301 ccacgatcgg tggtggccgc cgcggtgcgc gcctatgtcg cgctggtgga gtcggagccg
     3361 aacctgtacg acttcctctt ccgggcgccg ctgctcgaga gcccggccgg ccggcccgat
     3421 ctcgcccagg cgaaggtgac gctcgacctt gccggccggg tccgggtgct tctggatcag
     3481 aacgcgacca ccacctatcc gaacggaacc acgcagttgt gggccgaggc gctgctcggg
     3541 ctggtgcgca cgggcgtcga cgtctggaag cggggcgatc gcgcgctctc cgcggagcag
     3601 gtcaccgaac tcctgaccga actcgcctgg cacggcgcct cgacggcgtt ccacaccaag
     3661 gggccgcgtc ccgtgtagcg acggcgggtc ccggtgtggg gtgcgccgac tccgtcgcgt
     3721 cgagggcggc ctcgtcgtag gtgccacacg accagggcca ccacgacaac gagtcccgaa
     3781 ccgatcgccg tgccctctcc cagccggcgg gcgatgcgct cgtagctcgc accggccagg
     3841 tagccgatca ggaccacggt ggtcccccac gtgatgccgc cgacggcatt ccatgtgaga
     3901 aaacgggcat acggcatccg gcacgagccg gcgaaagtcg gcatcagcgc tcgaaggaat
     3961 gccgtccacc ggccggcgaa cacggccagg ccaccacggg tgcggaggaa ctcttgggcg
     4021 cgttcgacgc gtccggcgtg cgcgcgcagc cgccgctgac cgaacacccg cgtcagcaac
     4081 tcgcgaatgg ctccattgtg aggagcgggc tgggagaacc ctgagcttgt ctgtcggtcg
     4141 acgtgggtgg atgtgaaggg actcgaaccc ctgacctctc gcgtgtgaag cgaacgctct
     4201 aaccaactga gctacacatc cgggcgccga agcggcctgc tgaggatacc cgccggcgcg
     4261 ggcgttgcgg aaatcggttc gggtcagagg ccggcgaagg tgtgcaacca gtcctcggcg
     4321 gcgtcgggca tgaagctcgg gacgccggag aggcggaaga gcacccagaa gaacgtcagg
     4381 acggcggcca gggtgacgag ggcggcgagc ccctgcaccc agaggggctg gcccttgacc
     4441 cagacgttcc aggcccggac cttatcgcgc acccagtcca gcagggcctg cgcccactcg
     4501 aactcgctcg cccacacggc caggccggcg aagacgatca gccagcccgg gccgggcagc
     4561 ggcaccagcg ccaggccgcc ggcgacgatg atcaggccga ggagcccgac gccgagccgg
     4621 tagagcttgg ccgtcgcggg gttggcgcgg agcttgcgcc gccactccca gtcgtcgtcg
     4681 ttcttgtcga ggaagatgtt gccgtcgtgg cgggcgatct cgttgcggtc caaggattct
     4741 tgcgtcatct ccggttcccc cctctggtct tggccggtca cggatcgacc tccgccgccg
     4801 agcgttcggc gaagatcgcc atcgccgtgc gggtgacccg gccgggcgcg gcgagccggt
     4861 ggtcgtcgat ccgctcgatc ggatagacat cgcgcaggct ggaggtcacg aagacctcct
     4921 cgcacgtgcg caacacgccg agcggcatgg cgtcctcgaa gacctccatc ccggcctcgc
     4981 ggcaccactc gatcgtcagc tcccgggtga tcccggccag gcagcccgac gacagcggcg
     5041 gggtgtgcag ccggccggcc acgacgacga agatgttggt ggcggtgcct tcgcacagtt
     5101 cgccgcgggt gttggcgaag atcgcctcgc tggccccgcg ctcgtgggcg aaggcgagag
     5161 ccacgacgtt ctcggcgtat gacgtcgtct tcagtcccgc gaccgcggac cgttcgttgc
     5221 gggtccacgg ggtgacggcg agggtgccgg gcgggtgcgg cggggcctgc gggccggccg
     5281 tcacgacata cgtcagcggc gagtcctccc ggtcgctgcc gagcggcccc cgccccgcgg
     5341 tcacggtgaa ccgcagccgt ccgaaggcga tgggcccgcc cgagccgagc acggccgcga
     5401 tgccctcgtc gatgcggtcg aagtcgatgt ccgcgagccc gagaccgttg gcgctgcggg
     5461 ccattcgcgc cttgtgccgg ctcagggcga agacgcgtcc gtcgaggatc ttgcaggtct
     5521 cgaagatgcc gtcaccgacc gtgatcccgt ggtcgaccgc cgagatcgcg ggcgcggcag
     5581 ggtcgtgcaa cgcaccgttg acccaggccc gaatcacggt gctcatggtg ccacggctcc
     5641 cgaagccagt ccgatcagcc ggcgggcctt caactgcgtt tcctcccatt cggcctccgg
     5701 atccgaggcc cacgtgatcc cggccccggc accgaagtgc agcacgcggc gcccctgctc
     5761 ctcgctcgcc cagaacgtcc ggatgcccac ggcgagttcc gcctcacccg tgtcggcgtc
     5821 gatccagccg atcgcaccgc aatacggccc gcgcaccacc ggctccaggt cggcgatcgt
     5881 ggccaccgcc gacgacttcg gcgccccgga gaccgacccg ggtgggaacg tggcgtcgag
     5941 gatgtcgcgc caggagacat ccgggagcag ccgcccggcg acattcgtca cgaggtgcac
     6001 caggccagga tgattctcca tggcgcacag gtcctcgacg gcgacggtgc ccacgcggca
     6061 tacctgctgc aggtcgttgc gcaccaggtc ggcgatcatg acgttctcgg catagtcctt
     6121 gggcagcatc tcctccgggg tgcgcgaggt gcccttgatc ggtgaggaga ccagtcgctc
     6181 cccgtcccgc cgcaggtagc gctccggcga ggcgcacacg atgtcgacac cgaactcggg
     6241 cgcgtggatg agcgcttcgt agggcgccgg attgcccttg cggagcagac gcgccagtgc
     6301 gggcaggtag gcgtccgcgg gcaggtcgtg gctgagtacg cggcagaggt tgacctggta
     6361 gacagtgccg gcggcgatcc gctcgcggat ctcgcgcacc cccgccgtgt atgcagcccg
     6421 gtccatgctc gtgcgccacg tcccgtcgag cggctgccag ccgcgataac cgggcgggcg
     6481 cgtcatcgtc cgccgacccc ggtgggcgaa acgcaccgcg gtgagggtcg actcgaagtc
     6541 gacgaccacg gcccagaacc cctccgtcag ctcgtcgatg tcgtcgctga ccgcgagggg
     6601 ttcgtatgcc gtcatcgcgc cgaagcgtgc ccaggccacg gcgcgatcct atgggcgcgt
     6661 gatcccggcg gcgcgccttc ccgctcgggg cgcgccgccc gcctaccgtg gggagggcgc
     6721 ccacgctgcc caccgcccac gaaggatcgc ctgatgttcg agacaatcct cggtctgccg
     6781 ctgcacccgt tggtggtcca tgccgtcgtc gtgctcggcc cgctcgccgc gctgctgctg
     6841 gttgcgtatg ccgccgtccc ccgctggcgg gtggggctgc gcatccccgt gctggggctg
     6901 gcgctgatcg ctgcggggag cgcgttcgtc gcggatcagg ccggtgaggc gctcatgacc
     6961 gtccggggcg agccgggctt cgaccatgcc gagaaaggtg agctcgcgtt catctcgctg
     7021 ctggtgctgt tcctcgcgac catcgcggtg accgtgatcg ccaagccgtt gccggcggcc
     7081 acgaacatcg tgctgcccct ggtcatcgcc gtcgccgcag ccggcttcgc gctcttcgcg
     7141 gtcacccagg cggggcactc gggggcccag tcggtctggc agggcacctt ctccctcgga
     7201 gtgatcggcc ggtgacccgg cgccgctccc cgctcgccgc cgcccttgcc gtggcggtcc
     7261 tcgccgggtg ctccgcaacg gcgggcacgg ggcgcacgtc ggccggtgcc tcgccggccg
     7321 gggcgaacag cgtggctccc ccggccaagg acgtcgtcct cgcgttcggg ggcgacatcc
     7381 acttcgagag ctacctggcg aagctggcgc gcgacccggc cggactggcc gccctgcaga
     7441 agttgtgggc cgacgccgac ttccggatgg ccaatctcga gacggcgatc accgagggcg
     7501 gcacgcgggt ggtcaaggcg ttcaacttcc gggcgccggc gtcggcgctg acgaccctgg
     7561 ccggggccgg gatgagcgcc atgtcgctgg ccaacaacca cggcgtcgac tacgggctcg
     7621 acggtctcgc cgacaccctt gcggcgcgcg cgaattcgcc gttcccgatc gtggggatcg
     7681 gcgccgacga ggccgacgcg ttcgcgccga agatcgtgga cgtctcaggg ctgaaggtcg
     7741 ccctgctcgg cgcgagtcag gtcaacgagg agaccctcgc ccagttctcc gccgggcccg
     7801 gcaagccggg catcgccagc agtatgccgc ccactcgcct cgtcgatgcc gtcgcccggg
     7861 tgcgcgccca ggccgatgtc gtggtggtgt tcatgcactg gggcatggac tacaccgatt
     7921 gccccgacca gcgatccatc gacacctcca acgccctcgc cagtgccggt gccgacatcg
     7981 tcgtcggcgg tcacagtcac cgcatcaacg gcgccggctg gttgggcaag tcctatatcg
     8041 cctacgggct cggcaacttc gtgtggtggc gcgccaccga acccgactcg cgcaccggca
     8101 tcctgcgcct gacggtcgac cccgccctgg cgaaacagcc tgcagcccaa cgcaagacca
     8161 ccgtcgtcgt cgacgccacc tggcagccgc tgctcatcgg ccgcgacggc ataccagcca
     8221 ctcccccgcc tgccgaccac gaccgcctca tggacgtctg ggtcgccgcc ggccgctgca
     8281 gcggcctcac ccccaccccc tgacccccgt cgagtgaaca ccgaaaccgc gtcgagtgaa
     8341 caccgaaacc gcgtcgagtg aacatgcggt caatttggcc gagtaggttg gcagtatgct
     8401 cgccgtcgcc gccgtcaccc aatccaccga cgatcccctg tccggcctgc acgtcggtga
     8461 tattgacgaa ccgccggtcc ccgaggactg ggtgagcatc gacgtcgtga cgtgcgcgct
     8521 gaaccaccac gacctgtggt cgctgaaagg tgttgggctg ccgggcgatc ggctcccgat
     8581 gatcctcggc tgcgacgcgg ccggccaggc gccggacggg gccgaggtgc tggtctaccc
     8641 ggtgatctcg agtcccggat tccaggggga cgtcaccaac gacccgcgcc gctcgctcct
     8701 gtcggaggtc tatcccggca cccttgccga gcgggtccgc gtgccggccg gcaatctcgt
     8761 gcccaagccc gaggggctga gctgggagca ggcggcgtgc ttgccgacgg cgtggctcac
     8821 ggcataccgg atgctcttca ccaacgccgc cgtgcacccc ggcgacacgg tgctcgtgca
     8881 aggcgccggc ggcggagtcg cgacggccgc gatccagctc gcggcagcag cggggttgcg
     8941 cgtctgggcg acctcgcgcg atgcggccaa gcgtgacctg gcgaaagacc tcggcgcgac
     9001 ggagacgttc gaatccggtg cccgactgcc cgatcgcgtc gatgccgtga tggaaaccgt
     9061 cggcgcggcg acgtggtcgc actcggtcaa ctcgctacgc cccggcggca ccatcgtcat
     9121 ctccggtgcc acgtccggcg atgcccccgc caaggccgaa ctgaccaaga tcttcttcaa
     9181 gcagctgcgg gtgatcggct cgacgatggg cacgcgcgac gaactggcgc gcctcgcgcg
     9241 cttcgtcgcg gacaagggca tcgaaccgca gatcgactcg gtgctgccgc tggcgcaggc
     9301 ccgcaccggc ttcgaacgca tggccgccgg cgatgtcggc ggcaagatcg tcttccgcgt
     9361 gtagccgccg ccggctcaac gacgccgcgg gtctgacggg ccttgccagg gcaagcaacc
     9421 ctaggtagag tacgacctat gcgcacaccg cgaatgacgc aagcgacgca gctggtgctg
     9481 gacggtttcc tgcgagacga ggccgccgag cggcacggct acgagatcgg tgccgaggcg
     9541 gggttgccga gcggcacggt gcatccgatc ctggctcgtc tcgaagcgat cggc
//
LOCUS       DAOPJG010000132         9546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7518_length_9546_cov_7.667053, whole genome shotgun sequence.
ACCESSION   DAOPJG010000132 DAOPJG010000000
VERSION     DAOPJG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7518_length_9546_cov_7.667053"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..491
                     /locus_tag="PKU75_11685"
     CDS             39..491
                     /locus_tag="PKU75_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81585.1"
                     /translation="MTDIVKEDVRRDLIAHLLGGESATDDAIGVQAVAAEWVDDEASS
                     VDDISQRDAAGDLHALMQEHDDQQQALVAKARALDMTATETVRPGAIIGLDGAHYIVG
                     VASDEFTSGAMTYAGISVDAPLYGRLEGAKAGDTIAFNGHSSTIDYVA"
     gene            517..870
                     /locus_tag="PKU75_11690"
     CDS             517..870
                     /locus_tag="PKU75_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF81586.1"
                     /translation="MADFDARMIILSTENLDESIRLYSEVLGMPLKFRDGDHFAALDG
                     GSVTVALATAIDHPLPGQVVVGIKTPDVDAAAKAVEDAGGGIAKAAYDDAHERRSVVY
                     DHFGNGIVFYSPLQR"
     gene            873..1262
                     /locus_tag="PKU75_11695"
     CDS             873..1262
                     /locus_tag="PKU75_11695"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HPF81587.1"
                     /translation="MPERVSYRVYYEDTDSLGVVYYANYFKYFERGRSEFLRAHGHDV
                     ADLNARGFGLVVHAVSATFRKPAALGELVEVVTSFAVTSSYRGRFTQRVERDGELLVD
                     GVVDVVCLDGSGQLIEMPAELTALSGP"
     gene            1259..1987
                     /locus_tag="PKU75_11700"
     CDS             1259..1987
                     /locus_tag="PKU75_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPF81588.1"
                     /translation="MSRTRGRRRDRLWWLALPVAPIQGAWLTRTMVRFTDAEGVAGKV
                     GKGARQLVVVALGDSVTAGYAVAHHRSSIAGQLADRLAQRYAATVEWRVAARSGATAG
                     EAISLARPDVLGDADLVFVSAGVNDLKNLHRAAQFQREFGQLLDAVLAAAPRAQVCWL
                     GIPPLDHFPAFPRPLADVLGWRGRVFDAIGLAAVDARDRCFRIQSDGPLGLEMFGPDG
                     FHPSETLHAAFADAVMAALPPDAF"
     gene            complement(1988..3244)
                     /locus_tag="PKU75_11705"
     CDS             complement(1988..3244)
                     /locus_tag="PKU75_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81589.1"
                     /translation="MVGGLPVDESFPEAIINDVLRAAARELDRVTPTTLMGIDAGAVL
                     RSAADSFVNGVVARTGQEFAHGLRDALDAVSAQTYEGRASLGSLVLAPREHPAVSVDI
                     RFEHETPVTVPSLFRTVLEMSGPGLRLLTDGREVYGLGAINASYDEASEQCFLIDIVG
                     NGAWELRHQDEPLLRVDNGQPSVAVDAMDKGTFADTVRRVFGNQAEAEALWEMAQAWS
                     RQQHGTMLVVHPDAAAEGKRLLPQAFTIMPATLGERAFHTLTKIDGAVLVAPDAQCHA
                     VGVILDGAATGTGDGSRGARYNSAIRYLAGEGKGSMVIIVSEDGTIDLLPKLMRRVRR
                     ETVQAVVDQFAEAVADEEDYEKLARLNRACEKLEFYMTAPQCEAMNDARESIEERRWT
                     EERVRLQVVPVQPHPAMDDSYFVDPV"
     gene            3243..3848
                     /locus_tag="PKU75_11710"
     CDS             3243..3848
                     /locus_tag="PKU75_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81590.1"
                     /translation="MAGGDAWRVRLGIVLQCWRDHSRWRVRDLVRHLAGFYEPWSTPQ
                     HPRPWDTDALLDRVGLGDKAQSRIGDLSGGQRRRLDVAVGLVGRPELMWITALVAYAS
                     MAVSGIFYPITALPDWLAALGKCLPTHWIGLGYRSALLPPEAAALEAGGSWQTGLTAL
                     VLTGWALVGLSVAPRALLRLARRQSGSQVAAARDRIMSRGY"
     gene            3910..4116
                     /locus_tag="PKU75_11715"
     CDS             3910..4116
                     /locus_tag="PKU75_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF81591.1"
                     /translation="MLRAERGTSRRELADALGVHYQTIGYLERGEYSPSLHLALRIAG
                     YFDVPAEVVFSTEPFPRLGSGRAG"
     gene            4208..5908
                     /locus_tag="PKU75_11720"
     CDS             4208..5908
                     /locus_tag="PKU75_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPF81592.1"
                     /translation="MVSVTETYRAARDELLRLRGDHDRAVTEFRWPDLGDRFNWAIDW
                     FDAIARGNNQPALVVLEEDGSRAERTFDELATASDRLAAWLGERGVGKGDPVILMLGN
                     QVELWESMLAIMKLGAVIMPTTTALGLTDLTDRVERGGAKHVLCNAKDAYKFDGVPGE
                     FGRFSVGAVDGWEDITGVGADPVPPAAHPGTTPADPLLLYFTSGTTSRPKLVVHNQVS
                     YPVGHFSTVYWIGLQPGDRHLNISSPGWAKHAWSCFFAPWIAEATVVVVNYARFEAGP
                     LLARIREAGVTTFCAPPTVWRMLINADLSAGPGSLREVLSAGEPLNPEVIDQVEAKWG
                     LTVRDGYGQTETTAQVGNTPGSPVKHGSMGRALPGMPVALVDPLTHERVHPPAEGEVC
                     LDLSASPLALMDGYRGDDGRNAEAMAAGFYHTGDVASIDEEGYITFIGRTDDVFKASD
                     YKISPFELESVLIEHPAVAEAAVVPAPDPVRLAVPKAYIALVDGHAPDEATAKSILGY
                     AREHLAPFQRVRRIEFYELPKTVSGKIRRVELRQRENEGTDAHRGTEWRDDQFPNLLR
                     "
     gene            5940..8324
                     /locus_tag="PKU75_11725"
     CDS             5940..8324
                     /locus_tag="PKU75_11725"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPF81593.1"
                     /translation="MARSSGTPVNIDGHTVTLTNLDKVLYPETGTTKGEVIDYYRAVA
                     PWFIAHNSRRPATRLRFPDGVGTAATSVTPFFAKNVDSGTPSWVPTVTITHQHREADY
                     PLIDDAATLIWMAQIAALEIHVPQWRVGPRGGHHDPDRLVLDLDPGEGAGLAECVRVA
                     FLCKEILDGMSLPSVPVTSGSKGIHLYAGLDGRTSASVASQVAHQLALALEVSHPDLI
                     VSDMKRSRREGKVFIDWSQNNGHKTTIAPYSLRGRSRPYAAAPRAWDELTEDVTNLTF
                     TEVMERLRAGDDPLAVLLPTDAAVADAGDRLATYRSMRDGAKTPEPVPDSPPTATGVT
                     GNSFVIQEHHASRLHWDFRLEHEGVLVSWALPKGPPTDPKKNHLAVMTEDHPLEYGTF
                     EGTIPKGQYGGGEVAIWDAGTYDLEKWIDGREVIATLHGRPDGGLGGSAKFALIHTGK
                     GKDDRQWLIHRMTESHATTDSARATKMAAPQGDALPHIAPMLATAGDASDLRAGEWVY
                     ETKWDGYRAIAEVADGVAHFRSRNDKDFTRTYPELGELAALLDGHRAVLDGEIIALDP
                     SGRSSFGTLQNRGDVKAGIVAHYMAFDVLHLDGESLLRRPYRERRKVLESLGIDGKHI
                     HVPVTFGTDRELALRTSRELKLEGVVAKTVDGIYQPGARARTWIKLKHVYAQEVIVVG
                     WRPGEGRRAGGIGSLLIAVNEGGGLLYVGKVGTGFTEAALAEIRKRLDRIERKTPPLD
                     GVPRADAKDAHWVTPTLVGEVSFTEWTTTDRIRHPSWRGWRPDKDPKDVVRGAG"
     gene            8669..9472
                     /locus_tag="PKU75_11730"
     CDS             8669..9472
                     /locus_tag="PKU75_11730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPF81594.1"
                     /translation="MAGRLSHLPDQTATVRFLTAAAGLAGLAAPMVVAAILISRRPGL
                     WADVRSRLLWKRGTSRWYLLLVFLLMPASLVAAQAISLLFGYSGDQFLLRTEPSFTSG
                     LLPVWVILGLAPIVEEIAWHSYGTDTLITRMRLFWASLLFVVFWALWHLPLAMIKGYY
                     QAELVEEGWLTALNFPVSMIAFVFLMNWLYYRTGRSIAAAIIFHFVANYANEIFQTDP
                     DTKFIQTGLLLILTVVVVARNRELFFSRPARSQRREPVAPAPGPVPVNA"
BASE COUNT         1466 a         3353 c         3194 g         1533 t
ORIGIN      
        1 gcctccacca aggcgtctgc gtcaggcaga atcgggggat gaccgacatc gtcaaggaag
       61 acgtccgccg tgacctcatc gcccacctgc tcggcggcga gagtgccacc gacgacgcga
      121 tcggcgtgca agccgtggcg gcggaatggg tcgatgacga ggcctcctcc gtcgacgaca
      181 tctcccaacg cgatgcggct ggtgacctgc acgcgctgat gcaggagcac gacgaccagc
      241 agcaggcgtt ggtcgccaag gcgcgggcgc tcgacatgac tgccaccgag acggtgcggc
      301 ccggtgccat catcggcctg gacggcgctc actacatcgt cggcgtcgcc agtgacgaat
      361 tcacctctgg cgcaatgaca tacgccggga tctccgtcga tgcccccctc tacggcaggc
      421 tcgagggagc taaggccgga gacaccatcg ccttcaacgg ccactccagc acgatcgact
      481 acgtcgcctg accccctccc ggacgaaagg accaccatgg ccgacttcga cgcccgcatg
      541 atcatcttgt cgacagagaa cctcgacgag tcgatccggc tctattcgga ggtgctgggc
      601 atgccgctga agttccgcga cggcgaccac ttcgccgcgc tcgacggggg ctcggtcacc
      661 gtggctctcg cgaccgccat cgaccatccg ctccccgggc aggtcgtcgt cggcatcaag
      721 acgcccgacg tcgatgccgc ggccaaggca gtcgaggatg ccggtggcgg gatcgccaag
      781 gccgcttacg acgatgccca cgaacgccgc tctgtcgtct acgaccactt cggcaacggc
      841 atcgtcttct attcgcccct gcagcgctga tcgtgcctga acgcgtctcc taccgggtct
      901 actacgagga caccgattcg ctcggtgtcg tctactacgc caactacttc aagtacttcg
      961 agcggggccg cagcgaattc ctccgagccc acgggcacga cgtcgccgat ctcaatgccc
     1021 gggggttcgg cctcgtcgtc cacgcggtca gtgccacgtt ccgcaagccc gcggccctgg
     1081 gtgaactggt cgaggtcgtg acgtccttcg ccgtgacctc gtcctaccgc ggccggttca
     1141 ctcagcgggt cgagcgggac ggtgaactcc tcgtcgacgg ggtcgtcgac gtggtctgcc
     1201 tcgacgggtc cgggcaactc atcgagatgc cggcggagtt gacggctctc agcgggccgt
     1261 gagccgcacc cgcggtcgtc gccgggatcg gctgtggtgg ctcgcgctgc cggtcgcgcc
     1321 gattcaaggc gcgtggctca cccgaacgat ggtgcgcttc accgacgcag agggcgtcgc
     1381 cggcaaggtc ggcaaggggg cgcggcaact ggttgtcgtc gccctgggcg acagtgtcac
     1441 ggcggggtat gccgtggccc accaccgctc gtcgatcgcc ggccaactgg ccgaccggct
     1501 cgcgcagcgg tatgccgcga ccgtcgagtg gcgagtcgcc gctcgctcgg gtgccactgc
     1561 aggagaagcg atttcgctgg cgaggccgga cgtcctcggg gacgccgacc tcgtcttcgt
     1621 ctcggcgggc gtcaacgacc tgaagaacct gcaccgggcg gcgcaattcc agcgggagtt
     1681 cgggcagctg ctcgatgcgg tgctggcggc ggcgcctcgt gcccaggtgt gctggctcgg
     1741 cattccgccg ctcgatcact tcccggcgtt cccgcggccg ctcgcggacg tcctcgggtg
     1801 gcggggtcgc gtcttcgacg cgatcgggct ggcagcggtc gacgcgcgcg accgatgctt
     1861 tcgcatccag tccgacgggc cgctcgggct ggagatgttc ggccccgacg gtttccaccc
     1921 gagcgagacc ctgcacgccg ccttcgccga cgccgtcatg gccgcgctcc cgccggacgc
     1981 cttctgatca aaccgggtcg acgaagtagc tgtcgtccat ggcggggtgc ggctggacgg
     2041 gcacgacctg caggcgcacc cgctcctcgg tccagcgccg ttcctcgatc gactcccggg
     2101 cgtcgttcat ggcctcgcat tgtggtgcgg tcatatagaa ctcgagtttc tcgcaggccc
     2161 ggttgaggcg ggcgagtttc tcgtagtcct cctcgtcggc gaccgcctcg gcgaactggt
     2221 cgacgacggc ctggacggtc tcgcggcgca cgcggcgcat gagtttgggc agcaggtcga
     2281 tcgtgccgtc ttcgctgacg atgatgacca tcgagccctt gccctcgccg gcgagatagc
     2341 ggatggccga gttgtagcgt gcgccccggc tgccgtcgcc ggtgccggtc gccgcgccgt
     2401 cgaggatgac cccgaccgcg tggcactggg catcgggtgc gaccagcacc gccccgtcga
     2461 tcttggtcag cgtgtgaaac gctctctccc ccaacgtggc cggcatgatc gtgaaggctt
     2521 gtggcaacaa ccgcttgcct tcggcggcgg cgtcgggatg cacgacgagc atggtcccgt
     2581 gctgctggcg ggaccacgcc tgcgccatct cccacagggc ctcggcctcg gcctggttgc
     2641 cgaagacgcg gcgcacggtg tcggcgaagg tgcccttgtc catggcgtcg acggccacgg
     2701 acggttggcc gttgtcgacc cgcagcaacg gctcgtcctg gtgacgcagt tcccacgcgc
     2761 cgttgccgac gatgtcgatg aggaagcatt gctcgctggc ctcgtcatac gacgcattga
     2821 tcgcacccag gccatagacc tcccggccgt cggtgagcag gcgcagcccg ggcccggaca
     2881 tctcgagcac tgtgcggaac aggctcggga cggtcaccgg cgtctcgtgt tcgaagcgga
     2941 tgtcgacgct gacggccggg tgttcgcgcg gtgccagcac gagggacccg aggctcgccc
     3001 ggccctcgta ggtctgagcc gagacggcat cgagcgcgtc ccgcaggccg tgcgcgaact
     3061 cctggccggt ccgggcgacc acgccgttga cgaaggagtc cgcggccgac cgcaggacgg
     3121 cgccggcatc gatccccatg agcgtcgtcg gcgtgacccg gtccagttcg cgcgcggcgg
     3181 ctcgcagcac gtcgttgatg atcgcctccg ggaaggactc gtcgaccggc agcccaccga
     3241 ccatggccgg tggtgatgcc tggcgggtcc ggctcggcat cgtgttgcag tgctggcgtg
     3301 accacagccg ctggcgagtg cgtgacctgg tccgccatct cgccgggttc tacgagccgt
     3361 ggagcacgcc gcagcatccg cggccgtggg acaccgacgc cttgttggac cgcgtcggac
     3421 tcggcgacaa agcgcagtct cgcatcggcg acctgtccgg tgggcagcgc cgccgtctcg
     3481 acgtcgcggt cggtctcgtc ggccgcccgg aactgatgtg gatcacggcg cttgtcgcct
     3541 atgcctcgat ggccgtctcc ggcatcttct atccgatcac cgccctcccc gattggcttg
     3601 ccgcactggg caagtgcctg cccacccact ggatcgggct cggctaccgc tccgcgctgc
     3661 tcccgcccga ggccgccgcc ctcgaggccg ggggctcctg gcagacgggc ttgacggcac
     3721 tggtcctgac cggatgggcc ctcgtcggtc tgtcggtcgc accccgcgcc ttgctgcgct
     3781 tggcccgccg ccagtccggc tcgcaggtcg ccgcggcccg cgaccggatt atgtctcgcg
     3841 gctactgacc atgagactga gacctcgaag gaggatccgg tgaccgacca ggtacacaac
     3901 cgcatcgcca tgctgcgtgc cgagcgcggc acctcccgcc gcgaactcgc ggatgccttg
     3961 ggcgtccact accagacgat cggctacctc gaacggggcg aatactcccc gagcctgcac
     4021 ctcgccctgc gcattgccgg ctacttcgac gtgcccgccg aggtcgtctt ctccaccgaa
     4081 cccttccctc gcctcgggtc gggccgcgcg ggctgatccc gccctgcgtg tcggtatgcg
     4141 gtggctctag tctcgaaggg tgccttgggc cacgtcgtgc ggccgccgtt cacccgaagg
     4201 agtgtggatc gtgagtgtca ccgagaccta tcgcgccgcc cgcgacgagt tgctgcggct
     4261 gcgcggtgat cacgaccgtg cggtcaccga gttccggtgg cccgacctcg gggaccggtt
     4321 caactgggcg atcgactggt tcgacgcgat cgcccgcggc aacaaccagc cggccctcgt
     4381 cgtcctcgag gaggacggca gccgcgcgga gcgcaccttc gacgaattgg cgaccgcctc
     4441 cgaccggctc gcggcgtggc tcggtgagcg tggcgtgggg aagggggacc ccgtcatact
     4501 catgctcggc aaccaggtcg agctgtggga gtcgatgctc gcgatcatga aactcggcgc
     4561 cgtcatcatg ccgaccacga cggctctcgg cctgaccgat ctgaccgacc gggtcgagcg
     4621 cggcggcgcc aagcacgtgc tgtgcaacgc caaggatgcc tacaaattcg acggtgtccc
     4681 aggggaattc ggtcgattca gcgtcggcgc ggtggacggg tgggaggaca tcaccggcgt
     4741 cggcgcggac ccggtgccgc cggcggccca ccctggcacg acacccgccg acccgctcct
     4801 gctctacttc acgtccggga ccacctcgcg gccgaaactc gttgtgcaca atcaggtctc
     4861 ctatcccgtc ggacacttca gcacggtgta ctggatcggc ctgcagccgg gcgaccggca
     4921 cctgaacatc tcctcgcccg gttgggccaa gcacgcctgg tcctgcttct tcgccccgtg
     4981 gatcgccgag gcgacggtcg tggtcgtgaa ctacgcgcgg ttcgaggccg gaccgctgct
     5041 cgcgcggatc cgcgaggcgg gtgtgaccac gttctgcgcg ccgccgacgg tctggcggat
     5101 gctcatcaac gccgacctgt ccgccgggcc cggatccttg cgcgaggtgc tctccgcggg
     5161 tgaaccgctc aaccccgagg tgatcgacca ggtggaggcg aagtgggggc tgacggtccg
     5221 cgacggctac gggcagaccg agacgaccgc gcaggtcggc aacacgcccg gctcacccgt
     5281 caagcacggc tccatgggcc gggcgctgcc gggcatgccc gtggccctgg tcgatccgct
     5341 cacgcacgaa cgcgtccacc ccccggccga gggagaggtc tgtctcgacc tgtcggcctc
     5401 gccgctggca ctgatggacg gttaccgggg cgacgacggc cgcaacgccg aggcgatggc
     5461 cgccggcttc taccacaccg gtgacgtcgc gagcatcgac gaggagggct acatcacctt
     5521 catcggccgc accgacgacg tcttcaaggc gagcgactac aagatcagtc ccttcgagct
     5581 ggagtcggtg ctgatcgagc acccggccgt cgccgaggcg gcggtcgtgc ccgctcccga
     5641 cccggtgcgt ctcgccgtgc ccaaggccta tatcgccctc gtcgacgggc acgcgccgga
     5701 cgaagcgacg gccaagtcga tcctcgggta tgcccgtgag caccttgccc ccttccagcg
     5761 ggtgcggcgc atcgagttct acgaattgcc caagaccgtc tcgggcaaga tccggcgggt
     5821 cgagctgcgc cagcgcgaga acgaaggcac cgacgcccat cgcggcacgg agtggcgcga
     5881 cgaccagttc ccgaatctgt tgcggtgatc tcggcgcggt tgtcggcgac actgtcgaga
     5941 tggcgcgcag cagcgggacc ccggtgaaca tcgacgggca taccgtcaca ctcaccaacc
     6001 tcgacaaggt gctctacccc gagaccggga ccaccaaggg cgaggtcatc gactactacc
     6061 gtgccgtcgc gccgtggttc atcgcccaca actcgcggcg gcccgcaacc cgcctgcgct
     6121 ttcccgacgg ggtgggcacc gccgccacgt cggttacgcc cttcttcgcc aagaacgtcg
     6181 actccggcac gccgtcctgg gtgccgaccg tgacgatcac gcatcagcac cgggaggcgg
     6241 actatccgct catcgacgac gcggccacgc tgatctggat ggcgcagatc gcggccctcg
     6301 agatccacgt gccccagtgg cgtgtcggcc cgcgtggggg ccaccacgac ccggaccggc
     6361 tcgtgctcga cctcgacccg ggcgagggtg ccgggctggc cgaatgcgtg cgagttgcct
     6421 tcttgtgcaa ggagattctc gacggtatgt cgctgccctc ggtgcccgtg acgagcggca
     6481 gcaagggcat ccacctgtat gccggtctcg acggccgcac cagcgcgagc gtcgcctcgc
     6541 aggtggcgca ccagctcgcc ctcgctctcg aggtgtcgca tccggacctg atcgtcagcg
     6601 acatgaagcg atccaggcgc gagggcaagg tgttcatcga ctggagccag aacaacggcc
     6661 acaagacgac catcgcgccg tattcgttgc gcgggcgcag ccggccgtat gccgccgccc
     6721 cccgcgcgtg ggacgaactc accgaggacg tcacgaacct gacgttcaca gaggtgatgg
     6781 agcggctgcg cgcgggtgac gacccactgg ccgtgttgtt gccgacagac gccgccgtcg
     6841 cggatgccgg cgaccggctc gcgacctatc gatccatgcg cgatggcgcg aaaacccctg
     6901 agcccgtgcc cgacagcccg ccgacggcga ccggcgtgac cggcaactcc ttcgtcatcc
     6961 aggagcatca cgcgagtcgg ttgcactggg atttccggct cgaacacgaa ggcgttctcg
     7021 tgtcctgggc gctgcccaag ggcccgccga cagatccgaa gaagaaccac ctcgccgtga
     7081 tgacggagga tcacccgctc gaatacggca ccttcgaagg caccatcccc aagggccagt
     7141 acggcggcgg cgaggtggcg atctgggacg ccgggaccta tgacctcgag aagtggatcg
     7201 acggccgcga agtcatcgcg acgctgcacg ggcgacccga cggcggtttg ggcggcagcg
     7261 cgaagttcgc cctcatccat accggcaagg gcaaggacga ccggcagtgg ctgatccacc
     7321 ggatgaccga gagccacgcg acgaccgact cggcgagagc gacgaaaatg gctgcgccac
     7381 aaggtgatgc cctgcctcac atcgcgccca tgctcgccac cgcgggtgac gcctcggacc
     7441 tgcgcgccgg ggagtgggtc tacgagacga aatgggacgg ctatcgggcc atcgcggagg
     7501 tggccgacgg ggtggcgcac ttccgcagcc gcaacgacaa ggatttcacc cgcacctatc
     7561 ccgaactcgg cgagctcgcc gctctgctcg acggacaccg agcagtgctc gacggggaga
     7621 tcatcgccct cgacccgtcc gggcgctcca gcttcgggac gctgcagaat cggggcgacg
     7681 tcaaggccgg catcgtcgcg cactacatgg ccttcgacgt cctccatctc gacggcgagt
     7741 cgctcctgag gcgcccctac cgagaacgcc gaaaagtgtt ggagagcttg gggatcgacg
     7801 gcaaacacat ccacgtcccc gtcaccttcg gcaccgaccg ggaactggcg ctgcggacca
     7861 gccgcgagct caagctcgag ggtgtcgtcg cgaagacggt cgacgggatc taccagcccg
     7921 gagcccgcgc gcgcacctgg atcaagctca agcatgtcta tgcccaggag gtcatcgtcg
     7981 tgggctggcg accgggggaa ggccgccgcg ccggcgggat cggctcgctc ctgatcgccg
     8041 tcaacgaagg aggtggcctg ctgtatgtcg gcaaggtcgg caccgggttc accgaggccg
     8101 cattggccga gatccgaaag cgcctcgacc ggatcgagcg caagacgcca ccactcgacg
     8161 gcgtcccccg cgcggacgcc aaggacgccc actgggtcac gccgacgctg gttggagagg
     8221 tctccttcac cgagtggacc acgaccgacc ggatcaggca cccgtcgtgg cgggggtggc
     8281 gacccgacaa ggacccgaag gacgtcgtcc ggggcgccgg ctgaccccgg tgccgagggc
     8341 tggcgaggcg gtggcactcg gcacagccgt gacacacgtc cggctgcccg ctcgatctcg
     8401 tgccggtggg cctggtgtgg ccgtgaccgg ggtcatcccg ggtctcgcca tggtcggtcc
     8461 ctgagacacg tgccgacgct atttccccag gcgtaggttc gaagcgaacc gttcatcgtc
     8521 gttgggaagg aaaagcagtg agccgcaacc ggtctcatgt ggcacaggtc gagtcgagca
     8581 gcgcgggaat cgacccggtc gcctatcgct acgaccggcc ggtcacctct tcgtcctggc
     8641 gtcggcgatc acctgagcgc tctggctggt ggccggccgt ctcagccacc tccccgacca
     8701 gaccgcgacg gtccgcttcc tgaccgccgc cgccgggctg gccggcctcg cggctccgat
     8761 ggtcgtcgct gcgatcttga tcagccgccg gccaggtctg tgggcggatg tccgttcgcg
     8821 gctgttgtgg aagcgtggga cctcgcgctg gtacctactc ctcgtattcc tccttatgcc
     8881 ggccagcctc gttgcggcgc aagctatttc actgctcttc ggctacagcg gcgatcagtt
     8941 cctgttgcgc accgaaccca gcttcacctc gggtctgctg ccggtgtggg tgatcctcgg
     9001 cctcgccccg atcgtcgagg aaatcgcgtg gcacagctac ggcaccgaca ccctcatcac
     9061 ccggatgcgg ctgttctggg cctcgctcct cttcgtggtg ttctgggcgc tctggcacct
     9121 gccgctggcg atgatcaagg gctattacca ggccgagctc gtcgaggagg gctggctgac
     9181 agcgctgaac ttcccggtga gcatgatcgc tttcgtgttt ctgatgaatt ggttgtacta
     9241 ccgcaccggc cgcagcatcg ccgcggcgat catcttccac ttcgtcgcga actacgccaa
     9301 cgagatcttc cagaccgacc ccgacaccaa gttcatccag accggcctgc tgctgatcct
     9361 gaccgtcgtg gtcgtcgccc ggaaccggga actcttcttc agccgcccgg cacggagtca
     9421 gaggcgtgag ccggtcgcgc cggcgcctgg cccggttccg gtgaacgcct gacagccggg
     9481 ccggcgcgcc cggggaacca ccagttccag cgcccgagca gtcgcatggt cgccgggacc
     9541 agcagg
//
LOCUS       DAOPJG010000133         9531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7533_length_9531_cov_6.613761, whole genome shotgun sequence.
ACCESSION   DAOPJG010000133 DAOPJG010000000
VERSION     DAOPJG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9531
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7533_length_9531_cov_6.613761"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKU75_11735"
     CDS             complement(<1..546)
                     /locus_tag="PKU75_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81595.1"
                     /translation="MNDDAAERESLDDALFVLHGEEGTGTIRRLLYAGYLVLIFGGVY
                     GFNVLRAVLVTSDPQLVRSAVVSVWGVLALLAVLAGLVLAAWRAGRVRGPVVPPLPFI
                     DTVVASALDRWLVLRERGLALLVGGVGGGLLVGALVGGAFAGAGIGGMTGIAGAVLGG
                     GILGLVVALTWLAGQGAHSGAR"
     gene            complement(539..1204)
                     /locus_tag="PKU75_11740"
     CDS             complement(539..1204)
                     /locus_tag="PKU75_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81596.1"
                     /translation="MNGGSAAGPRVVADRVSKTIGVVTLLAPTSVTAEPGRALVVRGR
                     NGIGKTTLLKIVAGTLAATTGTVTIDGRPSDERDKTIRRRVAALLGAPAAYRDLTLRD
                     HLTLIDATWGGDSDTCEERVGDALGLLGIGDLAQRFPHELSSGQGQLFRLALTLFRPS
                     DLLILDEPEQRLDTDKRVLVGDLLAARRDAGTTLVVATHDPPLTERLADEVLDLGWGP
                     ADE"
     gene            complement(1287..1904)
                     /locus_tag="PKU75_11745"
     CDS             complement(1287..1904)
                     /locus_tag="PKU75_11745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF81597.1"
                     /translation="MGSPPRASVHDAAHWDQRYADPARVPFVAPEPVVTDVVSDVVTD
                     VVADVVAGVPGACGTALDIACGEGRHAVWLAEQGWRVTAIDFSAAGIATGRALGAAHG
                     VDVEWVVGDVRTWRPAAAYDLVLTCFVHLEEQTLRSVRSWLAPGGHLVLVSHGAGPGG
                     PGPRFRTTVAAMRERAGDLEVLECREVAGEAERSSRILLHARRPA"
     gene            1974..3539
                     /locus_tag="PKU75_11750"
     CDS             1974..3539
                     /locus_tag="PKU75_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HPF81598.1"
                     /translation="MARVTRTEELRERLLGYRPTDRLAGWLGPLLFAVLGGILRFWAL
                     DRPHQLVFDETYYVKQGVSMLDYGVEMRWNGEGKVVDPLFTHGNTKVFITDNGDMVVH
                     PPVGKWVIAFGEWLFGPTSSWGWRFSVAVLGTLSILMVGRIARRLFRSSFLGTLAAFL
                     TAFEGHHFVHSRTGLLDLILMFFALAGFGAILIDRDQMRERLAQHTGRLSRHTYEAWA
                     PLLGIRAWRWVAGLCLGLAAGTKWSGAFFLVGFGLMSVLWDMGAFRAAGIKHWVASAL
                     VVQGPLAFVAMVGTAAVTYLVSWTGWFLSDHGYKRHWADTHPSESFGWIPGSLRSLWS
                     YHQEAYTFHQGLTAPHDYMANPWSWIIQARPTSFFYESPKRGVDGCTVNECSKAISSL
                     GTVSIWWLAVIGIFFCLYHWIFRRDWRAGALLCGLAAGWLPWMDLQHRTIFQFYSIAF
                     EPYVVLIATFMLGLMLGRRGDPWPRRRRGLLAVGAFCLVTLSLFAFYWPIYTAQVVPY
                     SFWAAHMWFPSWI"
     gene            3710..7396
                     /locus_tag="PKU75_11755"
     CDS             3710..7396
                     /locus_tag="PKU75_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HPF81599.1"
                     /translation="MFILPGETRATISASDLRAAAACEWGLLRAFDVMLGRAEAVPGG
                     DDPVLERTVALGLAHEQRVLRELVRDHPGAVREIPRPPHTREGLEAAVAATRAALASD
                     ASVIYQAAFVAGRFAGYADFVVRDDAGRWQVWDTKLAREETVASLLQLASYADQLRSM
                     GVPVADDAVLVLGNGDHRTFPLDVILPVARSRMTRLLGLLDAHQDSGLPALWASEGVH
                     VCGRCPACEAELRAHDDVLLVAGVYTSQRKRLLAAGVTTMEELAERTDPVPDLPDSVL
                     SRVRAQARLQRRQAATGEVTSEIIDALPIAALPPPSAGDVFFDFEGDPMWSEPGSRSS
                     GLEYLFGVIEAPVGGAPPVFRAFWAHDRAQERQALRDFLDYVAERRKAFPDMHIYHYA
                     AYEKSALLRLAARYGVGEQEVDDLLREGVLVDLYAVVRAAVRVSQESYSIKKLEPLYM
                     GEELRRGPVATGGDSIVAYHEFVQARIEGREEEAAQRLESIRAYNEYDCLSTLRLRDW
                     LLAQADGSDAGVGLVQVDVDAGTSEAGGSEGGTGQAGGSGAGAGGVDAAPGADRPAGT
                     MWARAEALEQQLRELLGDKPAHERTRDEQALALVSAALQYNRREVKPYWWQHFDRLRH
                     PVDEWAGQAEVFLVGQAEVVAEWGKTGNQKVLRRRLEVTGRQGRGSRPARVGDSYQVV
                     YDVPPPPQLRPVSPALRVCETHATIIAVGAASTLAAPDQPGGDQTWILEETLPKKFEP
                     FAAAPLALVPGPPPGTKHLDAAVADIAEQVAAEWPDLPARAGLDILRRTPPRLLGDAE
                     LPGLDGSPEQFVTAITAATLALDHSYLAVQGPPGTGKTYVGAHVIARLVREHGWKVGV
                     VAQSHAAVENVLDAVVRAGLDGARVGKYAPGRVDPPWRHFADRDRDLAEFTTAHAEAG
                     YVVGGTAYDFTNPHRFAPGQLDLLVIDEAGQYSLATTLAASLVADRLLLLGDPQQLGE
                     VSQGQHPEPVARSALGWLIAGRDTLDPAYGYFLASTWRMHPRLAERISELSYAGSLGS
                     QDSVTAARSLDGVDPGVHVLPVAHRDNTVESVEEAQAIAARIGELLGAPWDDPDEPGG
                     PRPLVAGDFRVVTPYNAQVARVREVLDAAGLPEVPVGTVDKFQGQEGVIVFVSMAASA
                     RSDVARGMRFLLDRNRLNVALSRGKWAAYVVHSPELTDFTPSSAHELLLLGAFLRLTR
                     APDVAPATTSADAPTRTYEQVTLW"
     gene            7443..8210
                     /locus_tag="PKU75_11760"
     CDS             7443..8210
                     /locus_tag="PKU75_11760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPF81600.1"
                     /translation="MPSSKQSIRTLVAVALIAGTTAVTAPQASAKPESPASGPTTGAC
                     AFTPLEDQTYSTWTGLPPDPKKAQTHGKAAVVLQTNHGDIPVILDKAGAPCTVASFLY
                     LTKEKFYDGTQCHRLAAYHTAPYALSVLQCGDPLGTGWGDPGYWFKDELNTAKSLPNW
                     PGFEDGSRKNYTRGLLAMANGGPNTNGSQFFLVYEDSRLRPDYTVFGHITEEGMAVLD
                     KIAAAGVAPSDEGYPEDGAPAMTTIIERAKRTGAGGA"
     gene            complement(8421..8621)
                     /locus_tag="PKU75_11765"
     CDS             complement(8421..8621)
                     /locus_tag="PKU75_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81601.1"
                     /translation="MAPSEEDRSPTPEERAVLDALHLRKIDLADRVLIVNPGEYVGES
                     TRRELAYAQAAGKPITFTDPVA"
     gene            8556..>9531
                     /locus_tag="PKU75_11770"
     CDS             8556..>9531
                     /locus_tag="PKU75_11770"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPF81602.1"
                     /translation="MKSVQDGPLLGGGGPVLLAGGQDGPIPDSLTVTTANGETFTLNR
                     QQLTHAATIITVGNGITDVGRPGIKIALMAALTESTLRMLTNTGTYPESANYPNDGNG
                     GDHDSLGLFQMRPQSGWGTVAELMDPNYQARAFFGGPTGPNYPSPRGLLDIPGWQQMD
                     PGEAAQAVEVSAYPDRYRNYAPVADSILAALTNGGSTPVGVGGPAVSSSRVVFPLPEG
                     TWVLSSPFGMRVHPITGERKIHTGTDFAAPDGTPILAAADGTVTIAEFSGGYGGLIVI
                     EHTIDGKTVATAYAHMWQSGIHVRRGDRVSAGQHIGDVGSSGMSTGAHL"
BASE COUNT         1387 a         3508 c         3255 g         1381 t
ORIGIN      
        1 gcgagcgccg gagtgcgcac cctgtcccgc cagccaggtc agggccacca ccagccccag
       61 gatcccgccc ccgaggaccg cgccggcgat tccggtcatc ccgccgatac ctgcgccggc
      121 gaaggcaccc ccgacgagcg ccccgacgag cagcccgccc ccgacgccgc ccacgagcaa
      181 cgccagtccc cgctcgcgca acaccagcca gcggtcgagc gccgaggcga cgacggtgtc
      241 gatgaacggc agcggcggca ccaccggccc gcgcacccgg ccggcccgcc acgcggcgag
      301 caccagcccg gcgagcacgg cgaggagggc gagcacaccc catacggaca cgaccgcgga
      361 gcggaccaac tgcggatcgg acgtgaccag caccgcccgc agcacgttga acccgtagac
      421 cccgccgaag atgagcacga gatagccggc atagagcagc cggcggatcg tcccggtgcc
      481 ctcctctccg tgcaggacga agagggcgtc gtcgagcgat tcacgttctg cggcatcgtc
      541 attcatcggc gggaccccag ccgaggtcga ggacctcgtc ggcgaggcgt tcggtgagcg
      601 gcgggtcgtg ggtggcgacg accagggtcg tgcccgcgtc gcgtcgggcg gcgagcaggt
      661 cgccgaccag cacccgtttg tcggtgtcga gacgctgctc gggttcgtcg aggatcagca
      721 ggtcgctcgg gcggaacagg gtcagggcga gccggaagag ctgtccctgg ccggaggaga
      781 gttcgtgtgg gaagcgttgg gcgaggtcgc cgatgccgag caggccgagc gcgtcgccca
      841 cccgctcctc gcacgtgtcg gagtcgccgc cccaggtggc gtcgatgagc gtcaggtggt
      901 cccgcagcgt caggtcacgg tatgccgccg gcgcacccag cagggccgcc acccgccgcc
      961 ggatggtctt gtcgcgctcg tcgctgggcc ggccgtcgat cgtcaccgtg ccggtggtcg
     1021 ccgcgagcgt gccggcgacg atcttcaaga gggtggtctt gccgatgccg ttgcggccgc
     1081 gcacgacgag ggcccggccg ggttcggccg tcacggacgt cggcgccagc agcgtcacga
     1141 cgccgatcgt cttgctcacc cgatccgcga ccacccgcgg ccctgccgca ctccccccgt
     1201 tcataggggc accttaaggc gaatcgcgca cccgccatcg caccccagca gagaccacag
     1261 cagagagggc gcacgggtca ccgcgctcac gcggggcggc gggcgtgcag gaggatccgg
     1321 ctggaccgct cggcttcgcc ggccacctcc cggcactcga gcacctccag gtcgccggcg
     1381 cgctcccgca tcgccgccac ggtggtgcga aagcgcggcc ccgggccccc aggaccagcg
     1441 ccgtgcgaga cgagcacaag gtggccaccg ggcgcgagcc acgagcgcac gctgcgcagc
     1501 gtctgctcct ccagatggac gaagcacgtc aggaccaggt cgtatgccgc ggcgggccgc
     1561 caggtgcgca catcgccgac cacccactcc acgtcgacgc cgtgggcggc accaagcgcc
     1621 cggccggtcg cgatccccgc cgccgagaag tcgatcgcgg tgacccgcca cccctgttcg
     1681 gccagccaca ccgcgtggcg gccctcgccg caggcgatgt ccagagccgt gccgcacgcg
     1741 cccggcaccc ccgcgaccac gtcggcgacc acgtcggtga ccacgtcgct gaccacgtcg
     1801 gtgaccaccg gctccggcgc gacgaacggc acgcgggccg gatcggcata ccgctgatcc
     1861 cagtgcgcgg catcgtgaac gctggctctc ggcggactcc ccacccgtgc atcatcccca
     1921 ccgccgccac gggatgccag aagcggaccc gtcgtggggt ttcgcgacat acgatggcgc
     1981 gcgtgacccg gaccgaggag ctgcgcgagc gcctgctcgg ataccggccc accgaccggc
     2041 tggccgggtg gctcggcccc ctgctgttcg ccgtcctcgg cgggatcctg cggttctggg
     2101 cgctcgaccg gccccaccaa ctggtcttcg acgagaccta ttacgtcaag cagggcgtct
     2161 ccatgctcga ctacggggtc gagatgcgct ggaacggcga gggcaaggtc gtcgacccac
     2221 tcttcaccca cggcaacacc aaggtcttca tcaccgacaa cggcgacatg gtcgtgcacc
     2281 cgccggtggg caagtgggtc atcgcgttcg gggagtggct cttcgggccg acctcctcgt
     2341 ggggctggcg gttctccgtc gccgtgctcg ggacgctgtc gatcctcatg gtggggcgaa
     2401 tcgcccggcg gctgttccgc tccagcttcc tcggcaccct cgccgccttc ctcacggcat
     2461 tcgaggggca ccacttcgtc cactcccgca cggggctgct cgacctgatc ctgatgttct
     2521 tcgcgctcgc cggcttcggg gcgatcctca tcgatcgcga ccaaatgcgc gaacggctgg
     2581 cgcagcacac cgggcggctg tcgcggcata cgtatgaggc gtgggcgccc ctgctcggca
     2641 tccgcgcctg gcggtgggtc gccgggctgt gcctcggcct ggcggcgggg accaagtggt
     2701 cgggcgcgtt cttcctcgtc ggcttcgggc tgatgagcgt gctgtgggac atgggcgcct
     2761 tccgcgctgc cggcatcaag cactgggtgg cctcggcgct cgtcgtgcag ggaccactcg
     2821 cgttcgtcgc catggtgggc acggcggctg tgacctatct ggtgagctgg accggctggt
     2881 tcctctccga ccacggttac aaacggcatt gggcggacac gcacccgtcc gagagcttcg
     2941 gctggatccc gggatcgcta cggtcgctgt ggagctacca ccaggaggcc tacaccttcc
     3001 accaggggct gacggcgccg cacgactaca tggccaaccc gtggtcgtgg atcatccagg
     3061 cccgcccgac gtccttcttc tacgagagcc ccaagcgcgg cgtggacgga tgcacggtca
     3121 acgagtgctc caaggcgatc tcgtctctcg gtaccgtctc gatctggtgg ctcgcggtta
     3181 tcgggatctt cttctgcctg tatcactgga tcttccgccg cgactggcgc gccggggcgc
     3241 tgctgtgcgg gctcgccgcg ggctggctgc cgtggatgga cctgcagcac cggacgatct
     3301 tccagttcta ctcgatcgcc ttcgagccgt atgtcgtgct catcgcgacc ttcatgctcg
     3361 ggttgatgct cggccggcgc ggtgacccgt ggccacggcg gcgccgcggc ctgctcgccg
     3421 tgggggcctt ctgcctggtc accctaagcc tgttcgcgtt ctactggccg atctacaccg
     3481 cccaggtggt gccctacagc ttctgggccg cccacatgtg gttcccctcc tggatctgat
     3541 ccgtgcgaaa cccgttgggg cccaagtaac ttctgctagc agaggctagt caaatcagca
     3601 cagcttgcta ggtctgctga tttgactaac ctctgctgag ttcgctccgg agcgggccgg
     3661 tccggcctcc ggcgtcggac tgtcggaccc gcgacatatg gtctgaggcg tgttcatcct
     3721 tcccggcgag acgcgcgcga cgatcagcgc ctccgacctg cgcgccgccg cggcgtgcga
     3781 gtgggggttg ctgagggctt tcgacgtcat gctcgggcgg gcggaggcgg tgcccggcgg
     3841 cgacgacccg gtgctggagc gcaccgtcgc cctcggcctg gcgcacgagc agcgggtgct
     3901 gcgcgagctg gtgcgcgacc accccggtgc cgtgcgcgag atcccgcgcc caccgcatac
     3961 ccgcgaaggg ctggaggccg ccgtcgcggc aacccgggct gcgctggcct ccgacgcgag
     4021 cgtgatctat caggccgcct tcgtcgcggg acgcttcgcc gggtatgccg atttcgtcgt
     4081 ccgcgacgac gccggccgct ggcaggtgtg ggacaccaag ctggcgcggg aggagacggt
     4141 cgcgagcctg ctgcagcttg cgtcgtatgc cgatcagctg cggtcgatgg gggtcccggt
     4201 cgccgacgac gccgtcctcg tgctcggcaa cggcgaccac cggaccttcc cgctggacgt
     4261 catcctcccg gtcgcgcgct cccggatgac gcggctgctg gggctgctcg atgcccatca
     4321 ggactccgga ttgccggcgc tgtgggcgtc cgagggcgtg cacgtctgcg ggcggtgccc
     4381 ggcgtgcgag gccgagttgc gcgcccacga cgacgtcctg ctggtcgccg gtgtctatac
     4441 ctcccagcgc aagcgcctcc tcgccgccgg ggtgacgacc atggaggagt tggcggagcg
     4501 caccgacccg gtccccgatc tgcccgactc ggtgctgagc cgcgtgcgcg cgcaggcccg
     4561 tttgcagcgg cggcaggccg ccacgggtga ggtcacgtcc gagatcatcg acgccctccc
     4621 catcgcggcc ctgccgccgc cgagcgccgg cgacgtcttc ttcgacttcg agggcgaccc
     4681 gatgtggtcc gagccgggct cgcgctcgtc gggactcgaa tacctcttcg gggtcatcga
     4741 ggcaccggtc ggcggcgcac cgccggtgtt ccgggccttc tgggcgcacg accgggccca
     4801 ggagcggcag gcgctgcgcg acttcctcga ctatgtggcg gagcggcgaa aagccttccc
     4861 cgacatgcac atctatcact atgccgccta cgagaagtcg gccctgctcc ggctcgcagc
     4921 acgctatggc gtgggcgagc aggaggtcga cgatctcctg cgcgagggag tgctcgtcga
     4981 tctgtatgcc gtggtccgcg ccgcggtgcg tgtctcgcag gagtcatatt cgatcaagaa
     5041 gctcgagccg ctctatatgg gcgaggagct gcggcgcgga cccgtcgcca ccggcggcga
     5101 ctcgatcgtg gcctatcacg agttcgtcca ggcgcggatc gaggggcgcg aggaggaggc
     5161 cgcccagcgg ctggagtcga tccgcgccta caacgaatac gactgccttt cgacgctccg
     5221 gctgcgcgac tggctgctcg cccaggccga cggcagcgac gcgggagtcg gcctggtcca
     5281 ggtcgacgtc gatgccggga cgagcgaggc cggcgggagc gagggcggca cgggccaggc
     5341 aggcggtagt ggggcaggcg cgggcggggt cgacgcggcg ccgggtgccg accggccggc
     5401 cggcaccatg tgggcgcggg ccgaggccct cgagcagcaa ctgcgcgaac tcctcggcga
     5461 caaacctgcg cacgagcgca cccgcgacga gcaggcgctg gcgctggttt cggctgcgct
     5521 gcaatacaac cggcgcgagg tcaagcccta ttggtggcag cacttcgacc ggctgcggca
     5581 cccggtcgac gagtgggccg gccaggccga ggtcttcctc gtcgggcagg ccgaggtcgt
     5641 cgccgagtgg ggcaagacgg gcaaccagaa ggtgctgcgg cgccggttgg aggtgaccgg
     5701 ccggcaggga cgcggcagcc gtcccgcccg cgtgggcgac tcctaccagg tcgtctatga
     5761 cgtgcccccg ccgccacaac tgcgcccggt cagccccgcg ctgcgggtct gcgagaccca
     5821 cgcgacgatc atcgccgtcg gggccgcgtc gacgctggcg gcacccgatc agccgggcgg
     5881 cgaccagacg tggatcctcg aagagaccct gcccaagaag ttcgagccgt tcgccgccgc
     5941 cccgctggcc ctggtgcccg gcccaccacc gggcaccaaa cacctcgacg ctgccgtcgc
     6001 cgacatcgcg gagcaggttg ccgccgagtg gcccgacctg ccggcgcgcg cgggcctcga
     6061 catcctgcgg cggaccccgc cccgtttgct cggcgacgcc gagctgccgg ggctcgacgg
     6121 gtctcccgag cagttcgtga cggcgatcac ggccgcgacg cttgcgctcg accactccta
     6181 tctcgccgtc cagggcccgc ccggcacggg caagacctat gtcggggcgc acgtcatcgc
     6241 ccgcctggtg cgcgagcacg gttggaaggt cggggtcgtc gcccagtcgc atgccgcggt
     6301 cgagaacgtc ctcgacgccg tcgtgcgcgc cgggctcgac ggcgcgcgcg tgggcaagta
     6361 cgcgccgggg cgcgtcgacc cgccgtggcg gcacttcgcc gaccgcgaca gggacctcgc
     6421 ggagttcacg accgcccatg ccgaggccgg ctacgtcgtc ggcggcacgg catacgactt
     6481 caccaatccc caccggttcg cacccggaca gctcgacctg ctcgtcatcg acgaggccgg
     6541 tcaatactcg ctcgccacga cgctggcggc ctcgctcgtc gcggaccggc tgctgctgct
     6601 cggcgacccc cagcagctgg gcgaggtcag ccagggccag catcccgagc cggtcgcgcg
     6661 gagcgcgctg ggctggctga tcgccggccg cgacaccctg gacccggcat acggctactt
     6721 cctggcgagc acgtggcgga tgcacccccg cctggccgag cgcatctccg agctgtcgta
     6781 tgccggttcc ctcgggtcgc aggactccgt gaccgccgcg cggtcgctgg acggggtcga
     6841 tcccggcgtc cacgtgctac cggtcgcgca ccgcgacaac accgtcgaat cggtggagga
     6901 ggcgcaggcc atcgccgcgc gcatcggcga gttgctcggc gcgccgtggg acgatcccga
     6961 cgagccgggt ggtccccgcc ccttggtggc gggcgacttc cgggtcgtca cgccctacaa
     7021 cgcgcaggtc gcgcgggtgc gcgaagtcct cgacgcggcg ggtcttcccg aggtgcccgt
     7081 cggcacggtc gacaagttcc agggccagga gggcgtcatc gtcttcgtgt cgatggccgc
     7141 ctccgcgcgc tcggacgtcg cgcggggtat gcggttcctg ctcgatcgca accgactcaa
     7201 cgtggccctg tcgcggggca agtgggcggc gtatgtcgtg cactcccccg agctgaccga
     7261 cttcaccccc tccagcgccc acgagttgct gctgctcggg gcgttcctgc ggctgacccg
     7321 cgctcccgac gtggcgccgg ccacgacttc ggcggacgct ccgacgcgca catacgagca
     7381 ggtcacccta tggtgaggtg cggggcgacc cggcccctgg cataccaagg gaggcacaac
     7441 ccatgccgag ctcgaagcag tccatccgca ctcttgtcgc ggtagccctg atcgccggca
     7501 ccaccgccgt cacggcaccg caggcatcgg cgaagcccga atcgccggcg tccggcccga
     7561 ccaccggagc gtgcgcgttc acgcccctgg aggaccagac ctactcgacg tggaccggcc
     7621 tgccgccgga ccccaagaag gcccagaccc atggcaaggc ggccgtggtc ctgcagacca
     7681 atcacggcga catccccgtc atcctcgaca aggccggcgc cccgtgcacg gtcgcgagct
     7741 tcctctatct gaccaaggag aagttctacg acggcaccca gtgccaccgc ctcgccgcct
     7801 accacacggc gccctacgcg ctctcggtgc tccagtgcgg tgacccgctc ggcaccggct
     7861 ggggcgaccc gggctactgg ttcaaggacg aactcaacac ggccaagtcg ctccccaact
     7921 ggcccggctt cgaggacggc tcccgcaaga actacacccg cggcctgctc gccatggcca
     7981 acggcggccc caacaccaac ggcagccagt tcttcctcgt ctacgaggac tcgcggctgc
     8041 ggcccgacta caccgtcttc ggccatatca ccgaggaagg catggcagtc ctcgacaaga
     8101 tcgccgccgc cggcgtcgct ccgtcggacg agggctatcc cgaggacggc gcccccgcga
     8161 tgacgaccat catcgagcgc gccaagcgca ccggcgccgg cggcgcttga tcgtccccgg
     8221 ttgccccgcg gcaccggaaa cccctggacc agagggtcca ggggttttcg gtttggtggg
     8281 tggaggtacg tgcggacgtc cgcgtcgacg acctcgtaca ccggacgatg cgcctggccc
     8341 accccacaat ggatcagggc tcaacgcctc ggctccggct ccggcaattc gctcgatccg
     8401 gtagcgcagt gcgcgcacgg tcacgcaacg gggtcggtga acgtgatcgg cttcccagcc
     8461 gcctgggcgt aggccagttc ccttcgcgtc gactcgccga cgtactcgcc tgggttgaca
     8521 atcaataccc ggtcagccaa gtcgatcttg cgcaggtgaa gagcgtccaa gacggccctc
     8581 tcctcggggg tgggggaccg gtcctcctcg ctgggggcca ggacggcccg atcccggact
     8641 cgctcaccgt gaccaccgcg aacggcgaga ccttcaccct gaatcgtcaa cagctcacgc
     8701 acgcggcaac gatcatcacc gtcggcaacg gcatcaccga cgtgggccgc ccgggaatca
     8761 agatcgcgct gatggccgcg ctcaccgagt ccacgctgcg gatgctcacc aacaccggca
     8821 cctacccgga gtcggcgaac tacccgaacg acggcaacgg cggcgaccac gactccctcg
     8881 gcctgttcca gatgcgcccc caatcaggat ggggcaccgt cgcagagttg atggacccga
     8941 actatcaggc gcgggcgttc ttcggcgggc cgaccggacc gaactacccc tcaccgcgcg
     9001 gcctgctcga catccccggc tggcaacaga tggaccccgg cgaagccgcc caagccgtcg
     9061 aggtctccgc ctaccccgac cgctaccgca actacgcgcc cgtcgccgac agcatcctcg
     9121 ccgccctcac caacggcggc agcactccgg ttggcgtggg tggcccggcg gtctcgtcgt
     9181 cgcgggtggt attcccactg cccgagggca cctgggtgct gtcctcgccg ttcgggatgc
     9241 gggtgcaccc gatcacgggc gaacgaaaga tacacaccgg caccgacttc gccgcacccg
     9301 acggcacgcc gatcctcgcc gccgcggacg gcaccgtcac catcgccgag ttctccggcg
     9361 ggtacggcgg cctcatcgtc atcgagcaca ccatcgacgg caagaccgtc gcgaccgcct
     9421 acgcgcacat gtggcagagc ggcatccacg tccgccgcgg agaccgcgtg agcgccgggc
     9481 agcacatcgg cgatgtcggc tcctcgggca tgagcaccgg cgcacatctg c
//
LOCUS       DAOPJG010000134         9470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7603_length_9470_cov_9.071588, whole genome shotgun sequence.
ACCESSION   DAOPJG010000134 DAOPJG010000000
VERSION     DAOPJG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7603_length_9470_cov_9.071588"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="PKU75_11775"
     CDS             <1..1696
                     /locus_tag="PKU75_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF81603.1"
                     /translation="KPIKKFTERELAALLYKEPTKIKVDGINITYEGLIPRIQKSMLS
                     KDREAMQPHIRAFVDRAVVFQVCPECEGTRLNAAARSSKIKGINIAQACQMQISDLAE
                     WVRELEEPSIAPLLSGLQETLDSFVEIGLGYLSLDRPSGTLSGGEAQRTKMIRHLGSA
                     LTDVTYVFDEPTIGLHPHDIDRMNTLLKQLRDKGNTVLVVEHKPETIAIADHVVDLGP
                     LAGTGGGEIVYEGDLAGLRASDTLTGRHLEDRAQLKDALRKPTGALEIRGAHENNLRD
                     VDVDIPLGVLVVLTGVAGSGKSSLIRGSVEKLDSVVTVDQSAIKGSRRSNPATYTGLL
                     EPIRKAFAKANGVKPALFSANSEGACETCNGAGVIYTDLVMMAGVASTCEDCEGKRFH
                     AGVLEYRLGELNIAEVLDLPVADALPYFSQGAAKTSAAQKILERLNDVGLGYVRLGQP
                     LTTLSGGERQRLKLAAQMADTGSVYVLDEPTTGLHLADVENMLAMLDRLVDSGKSVIV
                     IEHHQAVMAHADWIIDLGPGAGHDGGTVVFEGTPADLVADGRTLTGQHLAAYVARR"
     gene            complement(1771..2226)
                     /locus_tag="PKU75_11780"
     CDS             complement(1771..2226)
                     /locus_tag="PKU75_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPF81604.1"
                     /translation="MPEILDALESADDIYLDVIGQVRLATWSAGRVGIIGDAAACASP
                     AAGQGTTLGLVTAYVTAAQLATTAAVPAALTAAEQQLRAFAEGNQALGPANLKRMVLP
                     TERAVRSTLFALKVMNALPFTDLELGAVARKLARASAYDLPAVPDAAAR"
     gene            2237..2575
                     /locus_tag="PKU75_11785"
     CDS             2237..2575
                     /locus_tag="PKU75_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81605.1"
                     /translation="MTGRYRAHPWLGAIAPAGVPLRPSVYAWTEALLLALAGAPTGID
                     EMRLAMLLDAVVRGYAGVGQSDSPPPAWLGPALAERFPRLAGELGRDFSDLDAELDHA
                     VSVILRGVTP"
     gene            2641..4038
                     /locus_tag="PKU75_11790"
     CDS             2641..4038
                     /locus_tag="PKU75_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HPF81606.1"
                     /translation="MLLTLTTTHRPATDLGYLLHKHPDRVQEFAQSFGTATVFYPEAD
                     EARCTAALVLEIDPVRLARAFGRNAPDFTLAQYVNDRPYAASSLLATALADVFSTARS
                     GRCAARQDLADSAIPLQIAVPVVPCRGGPEMARRIFEPLGWQVEADPIPLDPAFPDWG
                     PSRYVALTLTGTLRLSDALSQLHILLPALDESKHYWQGPDEIDKLFRSGEGWLATHPD
                     RELITRRYLGRRGGLMREGLARLAEVGDETPEALEPSAEEETALPAARRPRLNEQRQE
                     AVVAALLASGAISVIDLGCGPGALLSQLVKQPQFRRVAGTDVSVRSLQIAARRLHVET
                     MSERQSDKLQLFQSALTYEDARFAGFDAAVVMEVIEHVDPSRHAAVERVVFGTARPGT
                     VVVTTPNREYNALYDGLTGLRHADHRFEWDRAQFREWAEGVGAAYGYSVAYQGIGDVD
                     PGLGTPTQMAVFTRE"
     gene            4031..6550
                     /locus_tag="PKU75_11795"
     CDS             4031..6550
                     /locus_tag="PKU75_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HPF81607.1"
                     /translation="MSEVAQARTIGVPEMGLVVLVGVSGSGKSSFARRHFLPTEVLSS
                     DFCRGLVADDENDQAASADAFDVLHHIAGVRLRRGRLTVVDATNVQQRSRADLVKLAR
                     EHDVLVDAIVLDVPEAVAQERNRAREERDFGPHVVRGQHRDLMRSLRKLEREGFRRVH
                     LLKGVEEIDKVAIVREKSWNDRRELTGPFDLIGDVHGCARELHTLLRQLGWDLQEEDG
                     RVVGARHPHGRTAVFVGDLVDRGPDTPGVLRLVMGMVEAGTALCVTGNHEAKLVRALR
                     GAKVTVAHGLAESLAQLDTESDEFRTQATKFMDRLIAHYVLDDGRLVVAHAGLKEAYH
                     GRSSKRVRAFALYGDTTGETDEFGLPVRYPWALDYRGRALVVYGHTPVPNAEWVNNTI
                     CLDTGAVFGGALTALRYPERELVSVPAEREWYAPSRPLAPAGVERVPTTLAIEDVTGT
                     RWLETEHAGKVKIPEENAAAALEVMSRFAVDPRWLIYLPPTMSPASASQMDGYLERPE
                     PAFEEFATWGVTRVVCEEKHMGSRAIAVIARDAEAAERRFGVTDGSTGAVYTRTGRSF
                     FDDTTALVDRLRDAVAPLFHELTTDWLALDCELLPWSVKALDMIRAQYAATGAAATAA
                     LPQAISALERAADRGLEVSDLLARTSARLDNARAFRAAYAAYCRPTDGLDGVTIAPFQ
                     ILAAEGRTLALTQSHEWHLAQLGRLDHPLIAPTRHRFVDLGSDTERAAAAQWWEELTG
                     AGGEGMVVKPAGLVAGRIQPGLKVRGREYLRIIYGADYTDSLGLLRQRQLGKKRNLAL
                     REHGLGVDAIDAFVRGEPLWKVHQLVFSVLALESEPVDPRL"
     gene            6856..8148
                     /locus_tag="PKU75_11800"
     CDS             6856..8148
                     /locus_tag="PKU75_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPF81608.1"
                     /translation="MSSLLQHKLMSATEAIDLLGGGQTVVVPTGAGEPPTLLTELSKR
                     RREFHGVTVSQILATCPFDYFDAETADNVRHSAYFLGGAARPGAREGYGDVVPTHFSM
                     IPEMLRRGVLPVDAVFSVCSPPNENDYVYLGLAADYTMAAISRARTVVLEMHPGVPQT
                     NGMCRVHLSEVTGIVESRGDLTEIGPNPIGPTEIAIAEHVAELIPDGATLQIGYGSIP
                     DAIVQQLTHKHDLGVHTEMFGNGLLDLIESGAVTNERKSLHTGQTVLTFAMGSLRLYE
                     YLHRNPKMLILPVDLVNDPYLAGQNDLLHSINGSLQIDLIGQCGSESLGSVPYSGTGG
                     QTDFVRAANRSRGGKSFIVLPSTAKGGTISRIVPVLAPGTHVTTPKNDTDLVVTEFGV
                     ARLRGKNLNQRARELIAIAHPDFRDELLHAATALNLVY"
     gene            complement(8149..9126)
                     /locus_tag="PKU75_11805"
     CDS             complement(8149..9126)
                     /locus_tag="PKU75_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81609.1"
                     /translation="MASTSAAIAWLCDSDPAIAWQTRRDILGQDEHEWHPVQDQVETT
                     GWGARLLAQRGADGLWAGGAFLPSGFTRELWEAEGQPWTATSYALTDLREYGLNPATR
                     VGGDIVRRVGANARWDHDDEPFWEGEAEECINGRTVADGAYFGADVSGIVERLLGQRQ
                     PDGGWNCERAKGSTRSSFHSTINVLEGLMQYAAHEGDSAQLRQARRSGEEFLLQRNLF
                     RRLSTGKPASEEILKITHPSRWHYDVLRGLDYFRAAGRYAGTRPDARLAPALDWLRSR
                     RLPDGRWSVDVRPRGRAWFHADDGEGMPSRWATLRALRVLTWAEESPAI"
     gene            complement(9207..>9470)
                     /locus_tag="PKU75_11810"
     CDS             complement(9207..>9470)
                     /locus_tag="PKU75_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81610.1"
                     /translation="VAGHTWGEKDFGYRTASDADDLLGRLTRLHRIQIEPALARGLCA
                     SVYTQLTDVEDELNGLLTYDRVDKLDRAAVRALLAPMTGHSRG"
BASE COUNT         1497 a         3261 c         3225 g         1487 t
ORIGIN      
        1 caagccgatc aagaagttca ccgagaggga actcgcggcc ctgctctaca aggagccgac
       61 caaaatcaag gtcgacggca tcaatatcac ctacgagggc ctgatcccgc gcatccagaa
      121 gtcgatgctg tccaaggacc gtgaggcgat gcagccgcac atccgggcct tcgtcgaccg
      181 ggcggtcgtc ttccaggtct gcccggagtg cgaggggacg cggctgaacg cggcagctcg
      241 ttcatccaag atcaagggca tcaatatcgc tcaggcctgc cagatgcaga tcagcgacct
      301 ggccgagtgg gtgcgcgaac tcgaggagcc gagcatcgcc ccgttgctga gcgggctgca
      361 ggagacgctc gactccttcg tcgagatcgg tctcggctat ctgtcgctgg accggccgtc
      421 cggcacgttg tcgggcggcg aggcgcagcg gaccaagatg atccggcacc tggggtcggc
      481 gctgacggat gtcacctatg tcttcgacga accgacgatc gggctgcacc cgcacgatat
      541 cgaccgcatg aacacgctgt tgaaacagtt gcgggacaag ggaaacaccg ttctcgtcgt
      601 cgagcacaag cccgagacca tcgccatcgc cgaccatgtc gtcgacctcg gcccgctggc
      661 cggcaccggc ggcggcgaga tcgtctacga aggggatctc gccgggctgc gggccagtga
      721 caccctcacc gggcggcacc tggaggaccg ggcgcagttg aaggacgcgc tgcgcaaacc
      781 cactggcgcg ctggagatcc gaggtgctca cgagaacaac ctgcgcgatg tcgacgtcga
      841 cattccgctg ggtgtccttg tcgtgctgac cggggtcgcg ggctcgggca agagttcgct
      901 catccgtgga tcggtcgaga aactcgacag tgtcgtcacc gtcgaccagt cggcgatcaa
      961 gggctcgcgc cggtcgaacc cggcgaccta caccggcctt ctcgagccga ttcgcaaggc
     1021 gttcgccaag gccaatggtg tcaaacccgc tctcttcagc gcgaattcgg aaggcgcgtg
     1081 cgagacgtgt aatggcgccg gagtcatcta caccgacctg gtcatgatgg ccggcgtcgc
     1141 gagcacctgc gaggactgcg agggcaagcg cttccacgcg ggcgtcctcg aataccggct
     1201 cggcgaactc aatatcgccg aggtgctcga cctgccggtg gcggacgcgc tgccctactt
     1261 cagtcaagga gccgcgaaaa cgtctgcggc acagaagatt ctggagcgcc tcaacgacgt
     1321 cggtctcggc tacgtccgtc tcggccagcc gctgacgacg ctctcgggcg gggagcgcca
     1381 acggctgaag ctcgcggcgc agatggcgga cacggggtcg gtctacgtgc tcgacgagcc
     1441 gaccaccggc ctgcatctcg ccgacgtcga gaacatgctc gcgatgctcg accggctcgt
     1501 cgacagcggc aagtcggtca tcgtcatcga gcaccatcag gccgtgatgg cacacgccga
     1561 ctggatcatc gatctcggcc cgggtgcggg gcatgacggt ggcacggtcg tcttcgaggg
     1621 cacgcccgcc gacctggtcg ccgacgggcg gaccctgaca gggcagcatc tggcggccta
     1681 cgtcgcgcgt cgttgacgca gaccctctac gctcgtgggt agccgtggct ggaggcgccc
     1741 cccgacgtcc ccagccacgg cgccgacggg tcaccgcgca gcagcgtcgg ggacggccgg
     1801 taggtcgtat gccgaggccc tggccagttt gcgggccacc gcgccgagct cgaggtcggt
     1861 gaaggggagc gcgttcatca ccttcaacgc gaagagcgtc gagcgcacgg cgcgttcggt
     1921 gggcagcacc atccgtttga ggtttgccgg gccgagtgcc tggttgccct cggcgaaggc
     1981 acgcagttgc tgctcggcgg cggtgagcgc ggccggaaca gcagcggtcg tggccagttg
     2041 ggcagcggtg acgtacgcgg tgacgagacc gagcgtcgtg ccctggccgg ctgcgggtga
     2101 ggcacaggcc gcagcgtcgc cgatgatgcc gacgcgcccg gccgaccacg tggccagccg
     2161 aacctgtccg atgacgtcca gatagatgtc gtccgccgac tcgagggcat cgaggatctc
     2221 cggcacacgc cagcccttga ccgggcgcta tcgggcgcat ccgtggctcg gcgccatcgc
     2281 gccggccgga gttccgctgc gtcccagtgt ctacgcctgg accgaggccc tcctgctcgc
     2341 cctggccggt gccccgacgg ggatcgacga gatgcggctc gccatgctcc tcgatgcggt
     2401 cgtgcgtggg tatgccggtg tgggccagtc ggattcgccc ccgccggcgt ggctcggccc
     2461 ggctctcgcc gagcgcttcc cgcggctcgc gggagagttg gggcgcgact tctccgacct
     2521 ggacgcagaa ctcgaccacg cggtctcggt catcctgcgg ggagtcacac cctgaaaccc
     2581 gcgtgaagta gctgtcggaa atagtgtgct gacctgaccg gtccaggaga gactgatccg
     2641 gtgctgctca ccctgacgac gacccatcgg ccggcgaccg atctggggta tctgctgcac
     2701 aagcaccccg accgggttca ggagttcgca caatccttcg gcaccgcaac ggttttctat
     2761 cccgaggcgg acgaagcacg gtgcaccgcg gcgctggtgc tggagatcga tccggtccgt
     2821 ctggcgcgcg cgttcgggcg caacgcgccg gatttcacgc tcgcgcagta tgtcaacgac
     2881 cggccgtatg ccgcgtcctc cctcctcgcg accgcgctcg ccgacgtctt cagcacggcg
     2941 cgctcgggcc ggtgcgcggc ccgccaagac ctggcggact cggccatccc gctgcagatc
     3001 gccgtgccgg tcgtgccgtg ccgcggtggc ccggagatgg cccgccggat cttcgaaccg
     3061 ctcggttggc aggtcgaggc ggacccgatt ccgctcgatc cggccttccc tgactggggt
     3121 ccttcgcgtt atgtcgcgct caccctcacc ggcacgctcc gcctgtcgga tgcgttgagc
     3181 cagttgcaca ttctgctccc ggcgctcgat gagtcgaagc actattggca gggcccggac
     3241 gagatcgaca agctgttccg ctcgggtgag ggctggctgg ccacgcaccc ggaccgcgag
     3301 ctgatcactc ggcgttacct cgggcgtcgg ggcgggctga tgcgggaggg gctggcccgg
     3361 ctggcagagg tgggggacga gacgcccgag gccctcgagc ccagcgcgga ggaggagacg
     3421 gcgttgccgg ccgcgcgccg gccacgcctc aacgagcagc gtcaggaggc ggtcgtggca
     3481 gcattgctcg ccagcggcgc gatctcggtc atcgatctcg gctgcggccc tggcgcgctg
     3541 ctgtcacaac tggtcaagca gccccagttc cgccgggtcg ccggcacgga tgtctcggtg
     3601 cgctcgctgc agatcgccgc ccggcgcctg cacgtcgaga cgatgagcga acggcagtcg
     3661 gacaagttgc agctcttcca gagtgccctg acctatgagg atgcccggtt cgcgggtttc
     3721 gatgctgcgg tggtcatgga ggtcatcgag catgtcgacc cctcgcgcca cgctgccgtg
     3781 gaacgcgtcg tgttcggcac ggcgcggccc ggcacggttg tcgtgacgac ccccaaccgc
     3841 gagtacaacg cgctgtatga cggcctgaca ggcctgcggc acgccgacca ccgcttcgag
     3901 tgggaccggg cacagttccg ggagtgggcg gagggcgtgg gcgcggcata cggctattcc
     3961 gttgcgtacc aagggatcgg cgacgtcgat cccggcctcg gcactccgac gcagatggcg
     4021 gtgttcaccc gtgagtgagg ttgcccaggc gcgcaccatc ggggtgcccg agatggggtt
     4081 ggtcgtgctc gtcggggtct cgggcagcgg gaagtcctcc ttcgcgcggc gccacttcct
     4141 gccgaccgag gtgctgtcga gcgacttctg ccggggcctg gtggcggatg acgagaacga
     4201 ccaggcggcg tcggccgacg ccttcgatgt gctgcaccac atcgccggag tgcgcctccg
     4261 gcgcggccgg ctgacggtcg tcgacgcgac gaacgtgcaa caacggtcgc gggcggacct
     4321 ggtgaaactc gcgcgcgagc atgacgtgct cgtcgacgcg atcgtcctcg acgtgccgga
     4381 agccgttgcc caagaacgca accgcgcccg ggaggaacgc gacttcgggc cgcatgtcgt
     4441 gcgaggccaa caccgcgacc tcatgcgctc gctgcggaag ctggagcgtg agggctttcg
     4501 ccgggttcac ctcctcaagg gcgtcgagga gatcgacaag gtcgccatcg tccgggagaa
     4561 gtcgtggaac gaccggcgcg agctgaccgg cccgttcgac ctcatcggcg acgtgcacgg
     4621 gtgcgcgcgc gaactgcata ccctcctgcg acagctcggc tgggatctgc aggaggagga
     4681 cggtcgcgtc gtcggcgccc ggcatccgca tggtcgcacc gccgtcttcg tcggcgacct
     4741 cgtcgaccgt ggccccgaca cccctggcgt gctccgtctc gtcatgggca tggtcgaggc
     4801 ggggaccgcg ctgtgcgtga ccggcaacca cgaagccaaa ctggtccggg ccctccgtgg
     4861 ggcgaaggtc accgtggcgc acggactcgc cgagtccctc gcccagctcg acacggagag
     4921 tgacgaattc cgcacgcagg caacgaaatt catggatcgc ctcatcgccc actatgtgct
     4981 cgacgacggg cgcctggtgg tcgcccacgc cggcctgaag gaggcttacc acgggcgctc
     5041 gtccaagcgg gtgcgggcgt tcgcgctgta cggcgacacc acaggtgaga ccgacgagtt
     5101 cggtctcccc gtgcgctacc cgtgggcgct ggactatcgc ggccgggccc tggtcgtcta
     5161 cgggcacact ccggtgccga acgccgagtg ggtcaacaac acgatctgtc tcgacaccgg
     5221 agccgtgttc ggcggtgccc tgaccgcgct gcgctatccc gaacgcgagc tggtttccgt
     5281 tcccgcagaa cgcgaatggt atgccccgtc ccgcccgctc gcacccgccg gcgtcgagcg
     5341 agtgcccacc acactcgcca tcgaggacgt caccggcacg cgctggctcg agacggagca
     5401 cgccggcaag gtgaagatcc ccgaggagaa tgcggcggct gcgctggagg tgatgagccg
     5461 tttcgccgtc gacccgcgct ggctgatcta cctgccgccg acgatgtcgc cagcctcggc
     5521 atcgcagatg gacggctacc tggagcgccc cgagccggcc ttcgaggagt tcgccacgtg
     5581 gggcgtcacg cgcgtcgtgt gcgaggagaa gcacatgggc tcgcgcgcca tcgccgtcat
     5641 cgcccgcgat gccgaggccg ccgagcgccg attcggcgtc accgacggca gcaccggggc
     5701 cgtctacacc cgcacggggc gcagcttctt cgacgacacc acggcgctcg tcgaccggct
     5761 gcgggacgcc gtcgcgccgc tcttccacga gttgaccacg gactggctcg cgctcgactg
     5821 cgagttgttg ccctggtcgg tgaaggcgct cgacatgatc cgggcgcagt atgccgcgac
     5881 cggtgcggca gcgactgccg ctctgccaca agcgatctcg gcgctcgaac gggctgcgga
     5941 tcgcgggctg gaggtcagcg atctgctcgc gcggaccagc gcgcggctgg acaacgcgcg
     6001 cgcgttccgg gcggcttacg cggcatactg ccggcccacc gacggcctcg acggcgtgac
     6061 catcgcgccc ttccagatcc tcgctgccga aggtcgcaca ctcgcgctga cgcagtccca
     6121 cgagtggcat ctggcccagc tcgggcgtct ggaccacccg ctcatcgccc ctacccggca
     6181 ccgtttcgtc gacctcggct ccgacaccga acgggcagcc gccgcgcagt ggtgggagga
     6241 gctgaccggt gccggcgggg agggcatggt cgtcaagccg gccggcctgg tcgcgggtcg
     6301 catccagccc ggtctgaagg tgcggggccg cgagtatctg cggatcatct acggcgccga
     6361 ctacaccgac tccttgggtt tgttgcgcca gcgtcagctg ggcaagaagc gcaatctcgc
     6421 tctgcgagaa cacggactag gcgtggacgc gatcgacgcc ttcgtccgtg gtgaaccgct
     6481 gtggaaagtc caccagctgg tcttctccgt cctcgcactc gagtccgagc ctgtcgaccc
     6541 gcggctgtag cggcgaccac agggcgagcg aaagtgaccg gtcgcgccag aatgcgaccg
     6601 ctagcagggc gtggatctcg tgttcagcgc caactaccag ggcatccaga cctcgtacgc
     6661 cggtgtcgac gcagctcaca cggccgctgc gggagcctag gtgttggaga cggggcacct
     6721 ggtcatgtcc ggcgatgcat cggagttgtt ggcggacagc cggattcagg agaactatct
     6781 ggggatcgcg tgagttgggc agcatcacgc gggcaagccc tggcgcccgc cggcgcggct
     6841 cgatagtttc ggtgtatgag cagcttgctc cagcacaagc tgatgtcggc gaccgaggcc
     6901 atcgacctgc tcgggggcgg tcagaccgtc gtcgtgccga ccggggcggg ggagccgccc
     6961 accctcctca ccgagttgtc caaacgccgg cgtgagttcc acggcgtcac ggtctcccag
     7021 atcctcgcca cctgtccgtt cgactacttc gacgcggaga cggccgacaa tgtccgccac
     7081 agcgcctact tcctcggagg cgccgcccgg ccgggggcga gagaggggta cggcgacgtc
     7141 gtgccgaccc acttctcgat gatcccggag atgctgcggc ggggagtcct gcccgtggac
     7201 gccgttttca gcgtgtgttc gccccccaac gagaacgact acgtctacct cggtctcgcg
     7261 gcggactaca cgatggcggc catctcccgg gcgcggaccg tcgtcctgga gatgcacccc
     7321 ggcgtgccgc agaccaacgg gatgtgccgg gtgcacctgt cggaggtgac cggcatcgtc
     7381 gagagccggg gcgatctgac cgagatcggc cctaatccca tcgggcccac cgagatcgcg
     7441 atcgccgaac acgtcgccga gctcatcccc gacggcgcga cgctgcagat cggctatggc
     7501 tcgatcccgg acgccatcgt ccagcaactg acgcacaaac acgatctcgg agtgcacacc
     7561 gagatgttcg gcaacggcct gctcgacctc atcgaatcag gtgccgtgac caacgaacgc
     7621 aagtcactgc ataccggcca gaccgtgctg accttcgcca tgggcagcct ccgcctctac
     7681 gagtatctgc accgcaaccc gaagatgctg atcctgcccg tcgatctcgt caacgacccc
     7741 tatctggcgg gacagaacga cctcttgcac tcgatcaatg gatccctcca gatcgacctc
     7801 atcgggcaat gcggctcgga atcccttggc agcgttccct attcgggcac cggcggacag
     7861 accgacttcg tccgtgccgc caatcgctcc cgaggcggca agtccttcat cgtcctgccc
     7921 tcgaccgcca agggcggcac gatctcgcgg atcgtcccgg tgctcgcccc ggggacccac
     7981 gtgacgactc ccaagaacga caccgacttg gtcgtcaccg agttcggggt ggcgcgactg
     8041 cgcggcaaga acctcaacca gcgtgcccgc gagctcatcg ccatcgcgca tcccgatttc
     8101 cgcgacgaac tcctgcacgc ggccacggcg ctgaacctcg tctactgatc agatcgccgg
     8161 gctctcctcg gcccaggtca ggacgcgcag ggcacgcagg gtggcccagc gcgacggcat
     8221 accctcgccg tcgtcggcgt ggaaccacgc gcggccccgc ggccgcacgt ccacggacca
     8281 ccgcccgtcg ggcaggcggc ggctgcgcag ccagtccaag gcgggcgcca gccgggcgtc
     8341 ggggcgggtt ccggcgtagc ggccggccgc ccggaagtag tcgaggccgc gcagcacgtc
     8401 gtagtgccag cggctcgggt gcgtgatctt caggatctcc tcgctggcag gtttgcccgt
     8461 ggaaagacgg cgaaacaggt tgcgctgcaa caggaattcc tcacccgagc ggcgcgcctg
     8521 gcgcagctgc gcggagtcgc cctcatgcgc ggcatactgc atcaaaccct cgaggacatt
     8581 gatcgtgctg tggaaggagg accgggtcga gcccttggcg cgctcgcagt tccagccgcc
     8641 gtcgggttgc cgctggccga gcagtcgctc gacgatgccc gacacgtcgg cgccgaagta
     8701 tgcgccgtcc gccacggtcc ggccgttgat gcactcctcg gcctcgcctt cccagaaggg
     8761 ttcgtcgtcg tgatcccacc gagcgttggc gccgacccgg cgcacgatgt cgccgccgac
     8821 gcgcgtggcg ggattgaggc cgtactcacg caggtccgtc agggcgtagc tcgtcgcggt
     8881 ccacggttgc ccctcggcct cccagagttc tcgggtgaac ccggagggca ggaaagcgcc
     8941 acccgcccac agtccgtccg caccccgctg cgcgagcagg cgagcacccc agccggtcgt
     9001 ctcgacctgg tcctgcaccg gatgccactc gtgttcgtcc tggccgagga tgtcgcgccg
     9061 cgtttgccac gcgatcgccg gatcgctgtc gcacagccag gcgatcgccg cgctcgtgct
     9121 cgccatagac cccacaccac gagggtaggg aacgaaagcg cccaatcgcg gcaggagagt
     9181 cgggcatttt gctcactccg cggcggtcaa ccgcggctgt ggccggtcat cggtgccagg
     9241 agggcgcgga cggcggcgcg gtcgagtttg tcgacgcggt cgtaggtcag caagccgttg
     9301 agttcgtcct cgacatcggt cagctgcgtg tagacagacg cgcacaggcc gcgggcgagc
     9361 gccggctcga tctgtatgcg gtgcagccgc gtcaacctgc ccagcaggtc atcggcatcg
     9421 ctcgccgtgc gatagccgaa gtccttctct ccccacgtgt ggccggccac
//
LOCUS       DAOPJG010000135         9372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7726_length_9372_cov_7.692605, whole genome shotgun sequence.
ACCESSION   DAOPJG010000135 DAOPJG010000000
VERSION     DAOPJG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7726_length_9372_cov_7.692605"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1476
                     /locus_tag="PKU75_11815"
     CDS             193..1476
                     /locus_tag="PKU75_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPF81611.1"
                     /translation="MPLDYPIHERTLDNGLRVVISPDHSVPTVTVNLWVNVGSRHEVA
                     GRTGFAHLFEHLMFQGSRQVASGEHFSLLMGHGGRLNATTWFDRTNYFETIPVGVLDL
                     ALWLEADRHGGLLEAVNQENLDNQRDVVKEEKRQRYDNAPYGNAMRDVYATVFPDGHP
                     YHHPTIGSMADLDAASVEDVHAFFTAHYRPDNTVVTLVGDIEPEDGFARVARYFAGLP
                     ATPGESRPNLPQLPPLAAPARLDVVGDVPNDRLHIAFRLPVESDADFLAADLAIEAFA
                     GLSMSRLVRRLVREQQVANSVQGQAMGFVDGVSLGLIAIDLAEDADPAAVEEVFVEEL
                     ERFRAEGPSAVEMEAALAQAERAWLHALASQEERADLISHYALLHDDAGHINTLLDRY
                     AAVTADDVRRVAGAWLAPSSRAVVAYLTHENAEAA"
     gene            1476..2834
                     /locus_tag="PKU75_11820"
     CDS             1476..2834
                     /locus_tag="PKU75_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPF81612.1"
                     /translation="MTESLPARPDVAPAGPWSFPLPASTTLPNGLRVHTYDLPGQYVV
                     SLRLVVPLSVRAEPRDKEGVASLMSRLLDEGSTRHTTDEFTELLERKGVAFAAGLSDG
                     GMHVDIDAPARNLGAALELLYEAVAEPAFPEDQVRRMVRSRLAELEQERASAPHRAAR
                     ELAATFYDPSERASRPTAGSAETVRGVTRADIADFHAAHIGPTHASLVIAGDLSGIDV
                     PGLLAATLGEWTPLPVPVQPALTAPKQAGDALRIVLVDRPDSVQSEISVACAGPDRHV
                     EPAWAAYPVLGFVVGGSPSARIDAVLREEKGYTYGMRASFRPRRAGGLFVTSGSVRTE
                     VTTAALELVLDILDRAREGFEERERVEGVDFIRGTAPGRFATADAVAEEAAGLVLDGL
                     PMTFTTDNLEAMGALSVGDLDAAYRRFVDGRWCIVVVGDAAAYAEQIRALGRGAVTVV
                     AN"
     gene            complement(2845..3636)
                     /gene="hisN"
                     /locus_tag="PKU75_11825"
     CDS             complement(2845..3636)
                     /gene="hisN"
                     /locus_tag="PKU75_11825"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPF81613.1"
                     /translation="MPAYDDDLRLAHVLADAVERVTMPRFKATDLQVEAKPDLTLVSD
                     ADRTAEEVLRAQLGRTRPRDAVQGEEFPDTGHGPRRWVIDPIDGTHNFVRGVPVWATL
                     IALIDGDTPVLGLVAAPALGRRWWAAQGTGAWTGRSITSAKRIKVSGVRSLADASISV
                     SSVTSWAASGRQTPMLRLMERCWRDRGYGDFWSYMLLAEGAVDIAAEPELALYDMAAL
                     VPIVTEAGGRFTDLAGTDGPFGGNAVATNRLLHEQVLAALTPQAG"
     gene            3914..5599
                     /locus_tag="PKU75_11830"
     CDS             3914..5599
                     /locus_tag="PKU75_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPF81614.1"
                     /translation="MRGTTRTIAVAGVSALALLTAACNGGSSETTSATGGATGSASAS
                     GDTGASGGEITVNGCQPENPLIAGNTSETCGGDVLDTLTAKLVHYNSDTAEPEMDIAE
                     SIETDDNQNFTVKLKDYKFADGTPVTADSFVKAWNYTAYGPNGQGGSYFYGPIEGFDA
                     LQCTGTEEGKECEGTGKPKAEEMTGLKVVDDKTFTIKTTEKVSNLPVRLGYTAFAPQP
                     ESFFSDPEAFGKAPVGAGPFKFESWTEGQSIVVVKNPDYSGTNTAKLDKITFKFMQDM
                     DAAYNELVANQLDLLTAIPVSALIDDKYKTELTDRNAQRETATIQWIGMNPKADPTLA
                     KPELRKALSMAIDRETINKQIFNGTRTVSTGWVSPVVSGYKANACGEACVFDPAKAKA
                     MLEQAGGYDGKLTLTYNTEGDHKTWTTAVCNSITDTLGIECTATPGGEFGEFLTNLTE
                     GKTKGMFRNGWQMDYPSIENYLTPIYAKNADSNYFGPYDNPEFEKLLKEAAAAPSNDE
                     ANVKYQEAEAVLAEDFPTIPLFYGKATMGWSDKLNPVKITPFGTADFASISLK"
     gene            5840..6766
                     /locus_tag="PKU75_11835"
     CDS             5840..6766
                     /locus_tag="PKU75_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF81615.1"
                     /translation="MGKYIIRRLLQMIPVIIGTTFLIFYMVFALPGDPTAGKCGERPC
                     PAAYVAKYRAEHNLDDPMPIRYAKYVAKTATGDLGTNRYGVKVTEDLKKRYVVTGKLA
                     LMAVAVEVVIGIAAGILSGIRKGRFIDNLVLVSTLFVISIPVFVIGALCQYFLGVKWG
                     LFPVTVSAKPTLYELFMPAFVLGSLSIAYVARLTRTNLVENLRADYVRTAIAKGLSRK
                     RAVGLHALRNSMIPVITFVGFDFGGLLGGAIVTERIFNIQGIGGYIFRSIYNRDGVAV
                     VGAVTALVIVYLLVNLLVDLLYGVLDPRISHD"
     gene            6759..7685
                     /locus_tag="PKU75_11840"
     CDS             6759..7685
                     /locus_tag="PKU75_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF81616.1"
                     /translation="MTESATVVQAVEATNAAGDVGEARSLGADAWRALRKKPLFWVSL
                     VLITIFVLMAIWPSLFTNTDPDHAVLRQARERPNANAWFGRDIQGYDIYSRVVYGARA
                     SILVGVLTTTFTVLLGGAIGTLAAYAGGWVDALVSRVTDIFFAVPLLLGAILFMAATQ
                     PRGKPYFVIILLVVTAISILGWPSLARLMRSSVMQVLPNDYVQAARALGASPWRIIRK
                     HIIPNAMGPLIVVSTINLGAYIAAEATLSFLGIGLQPPVISWGVSISDAIVGLRTTPH
                     MLAFPSLFLSLTVLAFIMLGDAIRDAFDPKTR"
     gene            7719..8744
                     /locus_tag="PKU75_11845"
     CDS             7719..8744
                     /locus_tag="PKU75_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81617.1"
                     /translation="MRPANATGKITYTPVDGRLLDVEDLHVEFHTDDGIAKAINGVSF
                     TLDQGESLAILGESGSGKSVTAQAIMGLLDMPPAKIPHGAVKYCGVDLLTMPDDRRRW
                     VRGAEIAMIFQDALSSLNPVFPVGWQIAEMFRQHRGLNKSDAQAQAVRLMERVAIPAA
                     KERVKAYPHQFSGGMRQRIMIAMAIALDPAVLIADEPTTALDVTVQAQIMSLLQELQQ
                     ERQMGLILITHDLGVVADVADRSVVMYAGRLVETANVYDLYRNPAHPYTRGLLESIPR
                     LDQKGQTLAAIGGLPPNLLRIPPGCAFNPRCRMAKDVCRVDLPALREVAPGRHSACHF
                     AEEVIDA"
     gene            8737..>9372
                     /locus_tag="PKU75_11850"
     CDS             8737..>9372
                     /locus_tag="PKU75_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF81618.1"
                     /translation="MPEIILPKASEIGRSTTSEVILSAKDLVKHYPIKTGILRRTTGH
                     VKAVDGVSFDLHKGETLGIVGESGCGKSTLGRLLVRLEAPTDGSVEFEGADMAKASGR
                     ELRALRRNIQIVFQDPYTSLNPRMTVGDIVGEPLDIHPDVTPKGGRRKRVQELLDLVG
                     LNPEHINRYPHQFSGGQRQRIGIARGIALDPKVLICDEPVSALDVSVQAQVV"
BASE COUNT         1519 a         3358 c         3037 g         1458 t
ORIGIN      
        1 agctcgccgg gctcataacc cggaggtcgc aggttcaaat cctgcccccg ctactgcgaa
       61 tcgcgtcagc gtcgaagagc ccgggacgac atgtcgtccc gggctcttcg cttacccgcg
      121 ggtaggtacg acgcctcggc ggttataacc gcccaaccgt cttacctaaa ttcggggctc
      181 tatcgtgtcc ccatgcccct ggactatccg atccacgagc gcaccctgga caacgggctg
      241 cgcgtcgtga tctctcccga ccacagcgtc ccgaccgtca ccgtcaatct ctgggtgaac
      301 gtcgggtccc ggcacgaggt ggcgggtcgc accggtttcg cccacctctt cgaacacctg
      361 atgttccagg ggtcgcgcca ggtcgcgagc ggggagcact tcagcctgct catggggcac
      421 ggcggccggc tcaacgccac cacgtggttc gaccggacca actacttcga gacgatcccc
      481 gtcggcgtcc tcgacctggc cctgtggctc gaggcggacc ggcacggcgg gctcctcgag
      541 gcggtcaacc aggagaacct cgacaaccag cgcgatgtcg tcaaggagga gaagcgccag
      601 cgctacgaca acgcgcccta tggcaacgcc atgcgcgacg tgtacgcgac cgtcttcccc
      661 gacggccacc cctatcacca tccgacgatc ggatccatgg ccgacctcga cgccgcgagc
      721 gtcgaggacg tgcacgcctt cttcaccgcc cactaccgcc cggacaacac cgtcgtgaca
      781 ctcgtcggcg atatcgagcc cgaggacggc ttcgcgcgcg tggcacgcta cttcgccggc
      841 ctgcccgcca cgcccggcga gagccgcccg aacctcccgc agctgccgcc cctcgccgcc
      901 cccgcgcgcc tggacgtcgt cggtgatgtc cccaacgacc ggctgcacat cgccttccgg
      961 ctgcccgtcg agagcgatgc ggacttcctc gcggccgacc tggccatcga ggccttcgcc
     1021 gggttgtcga tgtcccggct ggtgcgccgg ctggtgcgcg aacagcaggt cgccaactcg
     1081 gtgcagggtc aggccatggg attcgtcgac ggcgtctcgc tcgggctgat cgcgatcgac
     1141 ctcgccgagg acgccgaccc ggcagcggtc gaggaggtct tcgtcgagga gttggagcgc
     1201 ttccgggccg aggggccgag cgccgtcgag atggaagccg ccctcgccca ggccgaacgc
     1261 gcctggctgc acgccctcgc ctcgcaggag gaacgcgccg acctgatcag ccactatgcg
     1321 ctgctgcacg acgacgccgg ccatatcaac accctgctcg accggtatgc cgccgtcacc
     1381 gccgacgacg tccgtcgggt cgcgggcgcc tggctcgcgc ccagttcgcg cgccgtcgtc
     1441 gcctatctga cccacgagaa cgccgaggcc gcctgatgac cgagagcctg cccgcccgcc
     1501 cggacgtcgc ccccgccggg ccgtggtcct tcccgttgcc ggcgagcacc accttgccca
     1561 acggcctgcg ggtgcacacc tacgacctgc ccgggcagta tgtggtgtcg ctgcgcctcg
     1621 tcgtgcccct cagcgtccgc gccgaaccgc gggacaagga gggcgtcgcg tcgctcatgt
     1681 cgcgcctgct cgacgagggg agcacacggc ataccaccga cgagttcacc gaactcctcg
     1741 agcgcaaagg ggtggccttc gccgccggcc tgtccgacgg cgggatgcac gtcgacatcg
     1801 acgcgccggc gcgcaatctc ggggccgcgc tggagctgct gtatgaggcg gtggccgagc
     1861 cggcgttccc cgaggaccag gtgcgccgga tggtgcgctc ccggctcgcc gagctcgagc
     1921 aggaacgcgc ctccgcgccg caccgggccg cccgcgagct cgccgcgacc ttctacgacc
     1981 cctccgagcg ggccagccgc cccaccgcgg ggagcgcgga gaccgtgcgc ggggtgacgc
     2041 gtgccgatat cgccgacttc cacgccgccc acatcgggcc gacgcacgcc agcctcgtca
     2101 tcgccggcga cctgagcggc atcgacgtgc cagggctgct ggcggcgacg ctgggggagt
     2161 ggacgcccct gccggtgccg gtgcaacctg ccctgacggc gcccaagcag gccggcgacg
     2221 ccctgcggat cgtgctcgtc gaccggccgg actcggtcca gtcggagatc tccgtcgcct
     2281 gcgccggacc ggaccggcac gtggagccgg cgtgggcggc atacccggtg ctcgggttcg
     2341 tcgtcggcgg ctcgccgagc gcgcggatcg acgccgtgct gcgcgaggag aagggctaca
     2401 cctacgggat gcgcgcctcc ttccggccgc ggcgggccgg cgggctcttc gtcacgagcg
     2461 ggtcggtgcg caccgaggtg accactgccg cactcgaact cgtcctcgac atcctcgacc
     2521 gggcccgtga ggggttcgag gagcgggagc gggtggaggg cgtcgacttc atccgcggca
     2581 ccgccccggg gcggttcgcg acggccgacg ccgtggccga ggaggcggcc ggcctggtgc
     2641 tcgacgggct gccgatgacg ttcacgaccg acaacctcga ggcgatgggt gccctgagcg
     2701 tgggcgatct ggacgcggca taccggcggt tcgtcgacgg ccggtggtgc atcgtcgtcg
     2761 tcggcgacgc cgcggcgtat gccgagcaga tccgcgcgtt ggggcgcggc gcggtgaccg
     2821 tcgtggcgaa ctagcgggcg ggggtcagcc ggcctgaggg gtgagggcgg cgaggacctg
     2881 ctcgtgcagc aggcggttgg tggcgacggc gttgccgccg aacggcccgt cggtgccggc
     2941 gaggtcggtg aaacgcccgc ccgcctcggt gacgatcggc acgagcgcgg ccatgtcata
     3001 gagggcgagt tcgggctcgg ccgcgatgtc gaccgcgccc tccgccagga gcatgtagga
     3061 ccagaaatcg ccatatcccc ggtcgcgcca gcacctctcc atcaggcgca gcatcggcgt
     3121 ctgacgtccg gaagcggccc acgaggtcac gctcgagacc gagatcgagg cgtcggccag
     3181 cgaccggacc ccggagacct tgatccgctt ggccgaggtg atgctgcggc cggtccaggc
     3241 gcccgtgccc tgcgccgccc accagcggcg ccccagcgcg ggcgccgcga cgagaccgag
     3301 gactggcgtg tcgccgtcga tgagggcgat gagcgtggcc cagacgggca ccccgcggac
     3361 gaagttgtgg gtgccgtcga tcgggtcgat cacccaccgg cgcgggccgt gaccggtatc
     3421 ggggaactcc tcgccctgga cggcatcgcg cgggcgggtg cggccgagct gggcgcgcag
     3481 cacctcctcg gccgtgcggt cggcgtcgct gaccagggtg aggtccggct tggcctcgac
     3541 ctgcaggtcg gtggctttga agcggggcat cgtgacccgc tcgacggcgt cggcgaggac
     3601 gtgggccaga cgcagatcgt cgtcgtatgc cggcatgagg gccacggtag tgggcggccc
     3661 agccgattcg cctcactggt cacaattttc ccttgcgcgt cgcgaccgac gccgaaatgt
     3721 cgtgcttgtg gcacggctgg gtcacatttc tgtctcggag gcgtcgtgat ggtcgccatt
     3781 ggcaggtacg tggacttaca taggggagcg cggcgcaccg cggcgcgcgc ggagcctcct
     3841 cgatcggggg acgcaggctc cgggcaaccg ccccgacccg gcggcggctt taaccccatg
     3901 taggaggaaa tccatgcgag gaactactcg cacgattgcg gtcgccggtg tttcggcgct
     3961 cgccctgctg acggcggcct gtaacggcgg ctccagcgaa accaccagcg ccacgggtgg
     4021 cgccacgggc agtgccagcg cgtccggcga caccggcgcc tccggcggcg agatcaccgt
     4081 caacggctgc cagcccgaga acccgctcat cgccggcaac acgagtgaga cctgtggtgg
     4141 tgacgtcctc gacaccctga ccgccaagct ggtccactac aactccgaca ccgccgagcc
     4201 ggagatggac atcgccgagt cgatcgagac cgacgacaac cagaacttca ccgtcaagct
     4261 caaggactac aagttcgccg acggcacccc cgtcacggcc gacagcttcg tcaaggcgtg
     4321 gaactacacc gcatacggcc ccaacggcca gggcggctcc tacttctacg gcccgatcga
     4381 gggcttcgac gccctgcagt gcaccggcac cgaagagggc aaggagtgcg agggcacggg
     4441 caagcccaag gccgaggaga tgaccggcct gaaggtcgtc gacgacaaga ccttcactat
     4501 caagaccacc gagaaggtct ccaacctgcc ggtccgcctg ggctacaccg cctttgcccc
     4561 gcagccggag tcgttcttca gcgaccccga ggccttcggt aaggccccgg tcggcgccgg
     4621 cccgttcaag ttcgagtcct ggaccgaggg ccagagcatc gtcgtggtca agaaccccga
     4681 ctactccggc accaacaccg ccaagctcga caagatcacc ttcaagttca tgcaggacat
     4741 ggacgcggcc tacaacgaac tcgtggccaa ccagctcgac ctgctcacgg ccatcccggt
     4801 ctcggccctg atcgacgaca agtacaagac cgagctgacc gaccgcaacg cccagcgtga
     4861 gaccgcgacc atccagtgga tcggcatgaa cccgaaggcc gacccgacgc tggccaagcc
     4921 ggaactgcgc aaggcgctct cgatggcgat cgaccgcgag acgatcaata agcagatctt
     4981 caacggcacc cgcaccgtct ccaccggctg ggtctccccg gtcgtgtccg gctacaaggc
     5041 caacgcctgt ggcgaggcct gtgtcttcga cccggccaag gccaaggcca tgctcgagca
     5101 ggccggcggc tacgacggca agctgacgct gacctacaac accgagggtg accacaagac
     5161 ctggaccacc gccgtgtgca acagcatcac cgacaccctg ggcatcgagt gcaccgcgac
     5221 cccgggcggt gagttcggtg agttcctcac caacctcacc gagggcaaga ccaagggcat
     5281 gttccgcaac ggctggcaga tggactaccc gtcgatcgag aactacctga cgccgatcta
     5341 cgccaagaac gcggacagca actacttcgg cccctacgac aacccggagt tcgagaagct
     5401 gctcaaggaa gccgccgccg ctccgagcaa cgacgaggcc aacgtgaagt accaggaggc
     5461 cgaggccgtc ctcgccgagg acttcccgac catcccgctg ttctacggca aggcgaccat
     5521 gggctggtcc gacaagctga acccggtgaa gatcaccccg ttcggcaccg ccgacttcgc
     5581 gagcatctcg ctgaagtgac ccgtggcggg ttcgtcacca cgtgactgat cggcctgccg
     5641 gtgattcgtc accggcaggc cgatcggctg cctgccaacg acttcgcggc gcccgaaaag
     5701 aaatcgaggg tcccgggttg gggtatcgga cgcgggtagg cgcgataggg tccggtacgc
     5761 attgactcgc tggagacttc cagctcacga actcgcacga ggccgagatc cggcctcgtc
     5821 acgacctgga ggaggtggca tgggcaagta catcatccgc cggctgctgc agatgatccc
     5881 ggtgatcatc ggcacgacct tcctgatctt ctatatggtc ttcgccctgc ccggcgaccc
     5941 cacggcaggc aagtgcggcg aacgcccctg cccggccgcc tacgtcgcga aataccgcgc
     6001 cgagcacaat ctcgacgacc cgatgcccat ccgctacgcg aaatacgtcg ccaagaccgc
     6061 gaccggcgac cttgggacca accggtatgg cgtgaaggtc accgaagacc tgaagaagcg
     6121 ttacgtcgtc accggcaagc tggccctcat ggccgtcgcg gtggaggtgg tcatcggtat
     6181 cgcggccggc atcctctcgg ggatccgcaa aggccggttc atcgacaatc tggtccttgt
     6241 ctcgacgctg ttcgtgatct ccatacccgt gttcgtcatc ggtgcgctct gccaatactt
     6301 cctgggtgtg aagtggggcc tgttccccgt gaccgtgtcc gccaagccca cgctctacga
     6361 gttgttcatg cccgccttcg tgctgggctc cctctcgatc gcctacgtcg cccggctcac
     6421 gcgcaccaac ctcgtcgaga acctgcgcgc cgactacgtc cgcacggcca tcgccaaggg
     6481 tttgtcgcgc aagcgcgccg tcggcctgca cgccctgcgc aactcgatga ttcccgtcat
     6541 cactttcgtg ggtttcgact tcggaggcct gctcggcggc gccatcgtca ccgagcgcat
     6601 cttcaacatc cagggcatcg gtggctacat cttccgcagc atttacaacc gcgacggcgt
     6661 cgcggtggtc ggtgcggtga ccgccctggt gatcgtctac ctactcgtca acctgctcgt
     6721 ggacctgctc tacggcgtcc tggacccgag gatcagccat gactgaaagt gcgactgttg
     6781 tccaagccgt cgaggcaacc aacgccgccg gcgatgtcgg cgaggcccgt tcgctgggcg
     6841 cggatgcctg gcgggccctc cgcaagaagc cgctcttctg ggtctcgctg gtgctcatca
     6901 ccattttcgt gctcatggcg atctggccca gcctgttcac caacaccgat cccgaccatg
     6961 ccgtcctgcg gcaggctcgc gagcgtccca acgccaatgc ctggttcggg cgggacattc
     7021 agggctacga catctactcc cgggtcgtct acggcgcgcg ggcctcgatc ctcgtcggtg
     7081 tcctgaccac gacgttcacc gtgctgctcg gtggcgcgat cggcacgctc gccgcctacg
     7141 ccggcggatg ggtcgacgcg ctcgtctcgc gcgtcaccga catcttcttc gccgtcccct
     7201 tgctgctcgg cgccatcctg ttcatggccg ccacccagcc gcggggcaag ccctatttcg
     7261 tgatcatcct gctggtcgtg accgccatct ccatcctggg ctggccgtca ttggcccggt
     7321 tgatgagatc gagcgtcatg caggtgcttc ccaatgacta cgtccaggcc gcacgggccc
     7381 tcggcgcaag cccgtggcgg atcatccgca agcacatcat ccccaatgcg atgggcccgt
     7441 tgatcgtcgt ctcgaccatc aacctcggcg cctatatcgc cgccgaggcc acgctgtcct
     7501 tcctcggcat cggcctgcaa ccgccggtca tctcgtgggg tgtctcgatc agcgacgcga
     7561 ttgtcggcct gcgcaccacg ccgcacatgc tggccttccc aagcctgttc ctgagcctga
     7621 ccgtgctcgc gttcatcatg ctcggcgatg ccattcgcga cgccttcgac ccgaagacgc
     7681 gatagggagg ctctgtgacg acaacaaccc agccgggcat ccgcccggcg aatgccaccg
     7741 gcaagatcac ctacaccccg gtggacggcc ggctgctcga cgtggaggac ctgcacgtcg
     7801 agttccacac cgacgacggg atcgccaagg ccatcaacgg cgtcagcttc accctggacc
     7861 aaggcgagtc gctggccatc ctgggtgagt ccggctccgg caagtcggtg acggcccagg
     7921 ccatcatggg cctcctcgac atgccgcctg ccaagatccc gcacggggcc gtgaagtatt
     7981 gcggcgtcga cctgctgacg atgccggacg accggcgccg ctgggtgcgg ggtgcggaga
     8041 tcgcgatgat cttccaggat gctctctcct ccctgaaccc ggtgttcccg gtgggctggc
     8101 agatcgccga gatgttccgg cagcaccgcg gcctgaacaa gtccgatgcc caggcgcagg
     8161 cggtccggct catggagcgc gtcgcgatcc cggcggccaa ggagcgcgtc aaggcctatc
     8221 cgcaccagtt ctccggtggt atgcgccagc gcatcatgat cgcgatggcg atcgcgctcg
     8281 acccggccgt cctcatcgcc gacgagccga cgaccgccct tgacgtgacg gtgcaggccc
     8341 agatcatgtc gttgctgcag gaactgcaac aagagcggca gatggggctc atcctcatca
     8401 cccacgacct cggcgtcgtg gccgacgtgg ccgaccggtc tgtcgtgatg tatgccggtc
     8461 ggctggtcga gaccgccaac gtctacgacc tgtaccggaa cccggcgcac ccctacacgc
     8521 gcggtctgct cgagtccatc ccgcgcctgg accagaaggg ccagactctc gcggccatcg
     8581 gtggcctccc gcccaacctg ttgcggattc cgcccggctg cgcattcaac ccgcgctgcc
     8641 ggatggccaa ggatgtctgc cgggtcgatc tgcccgcgct gcgcgaggtc gcgcccggcc
     8701 ggcactccgc ctgccacttc gccgaggagg tcatcgatgc ctgagatcat tctgccgaag
     8761 gcgtcggaga tcggtcggtc caccacgtcc gaggtcatcc tgagcgccaa ggacctcgtc
     8821 aagcactacc cgatcaagac gggcatcctg cgccgcacca ccggccacgt caaggccgtc
     8881 gacggcgtct ccttcgacct gcacaagggc gagaccctgg gcatcgtcgg ggagtccggg
     8941 tgtggcaagt ccacgctggg ccggctgctg gtgcgcctgg aggctccgac cgacgggtcg
     9001 gtggagttcg agggcgccga catggccaag gcctccggcc gcgagttgcg ggccctgcgg
     9061 cgcaacatcc agatcgtgtt tcaggacccg tacacctcgc tgaacccgcg gatgacggtc
     9121 ggcgacatcg tcggcgagcc cttggacatc caccccgatg tcacccccaa gggcggtcgg
     9181 cgcaagcggg tgcaggaact cctcgatctg gtcggcctca accccgagca catcaaccgt
     9241 tatccgcacc agttctccgg cggccagcgc cagcgcatcg gcatcgcccg ggggatcgcc
     9301 ctcgatccga aggtgctcat ctgcgacgag cccgtctccg cgctcgacgt ctcggtgcag
     9361 gcgcaggtcg tc
//
LOCUS       DAOPJG010000136         9233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_7894_length_9233_cov_6.134234, whole genome shotgun sequence.
ACCESSION   DAOPJG010000136 DAOPJG010000000
VERSION     DAOPJG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7894_length_9233_cov_6.134234"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..636)
                     /locus_tag="PKU75_11855"
     CDS             complement(22..636)
                     /locus_tag="PKU75_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81619.1"
                     /translation="MGAAVLAFVGAAATAFYGYHQVEWSTKNKYIQVFAIFFLYSLCA
                     ATLILLALWVKDQSSGTHQEELPNSPDTQISNSTTSFPNNFTVDFEPPSGTMSAIITG
                     GQTLLADWYSLEPPGCAASKLDLKVLSTPATDFDLKIYVSDASPHQLRVRVSQEGMRD
                     VYVTSTLPQRIRIKQGSAGRSLAFESVGALGAGCTEEIEFGLKY"
     gene            1041..1871
                     /locus_tag="PKU75_11860"
     CDS             1041..1871
                     /locus_tag="PKU75_11860"
                     /inference="COORDINATES: protein motif:HMM:NF017691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HPF81620.1"
                     /translation="MRGEVRQVLETSVPAHAVLDAADEVGAELVVAGLRRRTRWPNSS
                     SAGAPDSAAGRELSGARRQGRGGAADRDPIVGYDARPVHADRDQGRESRHQDQDRDQD
                     DNEDPDGHQNRRRRSTFRRTGGGGSVYYANCDAARAAGAAPVRIGDPGYRAGLDRDGD
                     GVGARTVQRCGTGQRRKEGSARHTVRLVFSRRPHVRIGQFRRPGQECMLWTSAQASSP
                     TGIAQRREGDAVALSTGVSCPLDRTKRPPYRREGEETGGQPTPRLRRHTDRCAPGRAP
                     "
     gene            1902..>4036
                     /locus_tag="PKU75_11865"
     CDS             1902..>4036
                     /locus_tag="PKU75_11865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPF81621.1"
                     /translation="MPTPRTSTPATTTKPKKKRRWLRRTLWTLFTLGLLGAIGLVIAY
                     IVTPIPKPNEAALQQSSTIYYSDGKTVMDRFAEVNRSIVPIDRIPKEVQEAFLAAEDR
                     SFYDNNGISPKGIARSIWVGLRGGPQQGGSTITQQYVKNYYLTQDRTLTRKFKEIMIS
                     VKIDGELSKDDILANYLNTIYFGRGASGIQTASQAYFGKSVNQLTVSEGALLASVIRG
                     PSYYDPRLGAEQKQNAKERWGYVLDGMVDQGWLTQAERDKAKFPATIKIRTNRIATGP
                     EGFLSEMVRAELQSKVGLTEDDLARGGYRITTTIDKRAQEAAKSAIAARMPQGVNDLH
                     VGLAAVAPGDGAIRALYGGAKYGSGDLGYFNTATQAAMQAGSTYKVWTLVAALERGIP
                     LSTRFDARSPMFFEQFKSTEPGATPAAAAGEVVNFGNHGYGVVDMATATGLSANTYYA
                     QLNIEVGPESTRDAAETAGLKAAGAQKELGDNYANVFGTDLVSVLGQANAYATIAAEG
                     RRATPYIISGVRATGQFKINYKADKQVKTVFDKAVMRDTIHAMARVTQAGGTGAAAAA
                     LGRPAAGKTGTTSDNYSAWFDGFTPGQLAAAVGIYKGDGSLVPENQMNAIPGVGELTG
                     GTVPVNIWTDFMRLALEGQPIAPLPPAGNVSGGSPTSVTTAPTTQSSTTTQSSSSSSS
                     SSSSESVTQTQTETTTTESSTPPPTTDTST"
     assembly_gap    4037..4136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4236..5534
                     /locus_tag="PKU75_11870"
     CDS             4236..5534
                     /locus_tag="PKU75_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81622.1"
                     /translation="MRPDVLPPRSTAFLGGPPGRYARERGWWRTIATWLVPLTAIPMA
                     LAVLQRERCIRYGWTGSDQFWRMCFSDLPAQYAIAGLDTGLTGYLRDATLDQPVLAGS
                     VMSLLGGLVPDGSAIDQSRSYFLYWVGLITLLLAACVWCTVHVVPDDLQAAAQVALSP
                     VIVLAAVLSSDALAVALVAGAMLAWHRDKVALTGILLGLGMMTRGYVALVAIALLLAA
                     RREGRLRAAWEAVLFAAATALFVAIFFIIAAPDALLAPYLNWWEGDAGYGSLWLIPSI
                     AGHALPAWAVALLAAAGWVLAVVLGWVFTRDAYRAPTWPQVALVVVGMGMMLGKTVPV
                     QAALWLLPIAAMAGMRWRDHLIWAGAEALHFMAVWLYVGAQTTPNRALPGAWYVVFLL
                     IRTAGITWLVTRVWQAGLFPPARHERHDDYQPVGKTVGRL"
     gene            complement(5552..6646)
                     /locus_tag="PKU75_11875"
     CDS             complement(5552..6646)
                     /locus_tag="PKU75_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81623.1"
                     /translation="MPPPPVPSPPPEGLPRGPWRAAVAHHFAVRHCGLARRADLLGAG
                     FTKAELEADLDAGRLITVGPNTIAVPTAAVPHWLGYGKKYDGHHLPLGVTGMAKDGRR
                     ARQLKATALMAPPRKAGPATVDTAFAPFWWAVWESGTRAALDGASALIAEGMSGFVLD
                     HIEISVPLGARIHPHPGVRITQRRVLGPLRPGLPRVEPATATVRAAQLAVSDRQAALL
                     ICLPVQQRIVRAEDLLARWTTVRRSPRRSFLDAVIKDVCDGAHSLGELDFARFCRERG
                     LPEPVRQQLRRGPKGRIYLDVAFECGLVVEIDGVQHSLGLAPVDDALRANAVTLQNRR
                     VLRIPVLGLRLDPGAFLDQVAHGIRLMTRG"
     gene            complement(6764..7078)
                     /locus_tag="PKU75_11880"
     CDS             complement(6764..7078)
                     /locus_tag="PKU75_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81624.1"
                     /translation="MPSAPVDYHRTSARPAFAALPAAVREAVAALAGASVASARPPVG
                     SGFTGSYAGVLDLADGRRVFAKAAGATMPHVVSGLRREGQVLPILASLSCVPHLVAAG
                     DE"
     gene            7277..7564
                     /gene="rpsF"
                     /locus_tag="PKU75_11885"
     CDS             7277..7564
                     /gene="rpsF"
                     /locus_tag="PKU75_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPF81625.1"
                     /translation="MRQYELMVILDPELDDRQVGPTMEKFLGVVKTDGGTVDNIDVWG
                     RRRLAYPIKKKDEGSYVVTLFTSEPATAKELDRQLGLSELVMRTKLLRADA"
     gene            7592..8218
                     /locus_tag="PKU75_11890"
     CDS             7592..8218
                     /locus_tag="PKU75_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPF81626.1"
                     /translation="MANETTITIIGNLTGDPELRFTPSGAAVANFTVASTPRTFDRNS
                     NEWKDGETLFMRCSVWREVAENVAESLHRGTRVIVSGRLKSRSWDDKETGQKRTVIEM
                     DVDEVGPSLRYATTQVTKTQRGGGNFGGGGDEGGWGQQSGGGQSGGGQPGGGQGGQGG
                     WGQQAPQSGNQGGGWGQQAPQSGQNGQGGQGNSGGWGGGAPSYDEPPF"
     gene            8396..8632
                     /gene="rpsR"
                     /locus_tag="PKU75_11895"
     CDS             8396..8632
                     /gene="rpsR"
                     /locus_tag="PKU75_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPF81627.1"
                     /translation="MAKPVVRRPKKKANPLKSAKIENIDYKDAALLRKFISDRGKIRS
                     RRVTGVSVQEQRLIAQAVKNAREMALLPYSSSSR"
     gene            8659..9105
                     /gene="rplI"
                     /locus_tag="PKU75_11900"
     CDS             8659..9105
                     /gene="rplI"
                     /locus_tag="PKU75_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPF81628.1"
                     /translation="MKVILTHEVTGLGTAGDVVDVKPGYARNFLYKRGLATAWSAGAQ
                     KQIDAIARGREARAVKSLEEAQSIKGNLEASTVQVAAHAGKEGRLFGAVSTADIAAAV
                     KAAGGPDLDRRTIEVPTPIKAVGSHTALVRLHPEVQANLTLDVVAG"
BASE COUNT         1548 a         3211 c         3046 g         1328 t
ORIGIN      
        1 tccgagatcc tttctgcctt atcagtattt caatccgaac tcgatttcct cagtacaccc
       61 agctccaagg gctccgacgg actcgaaagc gagggatcgc cccgcgctcc cttgtttgat
      121 cctgatcctt tgtggcaacg tactagtcac gtaaacgtcg cgcatcccct cctgtgacac
      181 cctgactcgc aactgatgcg gcgacgcgtc ggagacatag atcttgagat caaaatccgt
      241 tgccggagta gatagcactt tcaggtcgag tttggaggcc gcgcatccag gcggttcgag
      301 tgagtaccag tcagctagca gagtttgccc gcctgtaatt atggcactca tcgtcccgga
      361 tggcggctcg aaatccacgg taaagttatt ggggaagctt gtggttgaat tggagatctg
      421 tgtatcggga gagtttggaa gttcctcttg gtgggttcct gaggactggt ctttaaccca
      481 aagggcaagg agaattagtg tagcggcgca tagactatag aggaagaaga tcgcgaatac
      541 ttgaatgtat ttattcttgg tcgaccactc cacctggtgg tacccataga aggccgtcgc
      601 agccgcaccg acgaacgcta ggacagcagc acccatttgc cgtccttctc agccatgcac
      661 cacgtataca ccatgattcc gaccgctgcc acccaggacc cgcttgcagc aagactggat
      721 tgtcagtctg cctccgatca gcctgcaggc gcgtatcgtc cgggtcaggt ggcccgcgcg
      781 tggtggccat gacgccagtc aacgagcccc accccgctgt ggccgagggg cacggccggg
      841 ggatgccgcg cccacctgac ggtcaggtca gcggctcagg cgttgtggtg gcgagtgcgc
      901 cacgtggtga tggcaattcg gaagcggtgt ccgggagtga cgggagggcg gttctttcgg
      961 gcaggacgag gggatcgatt agcccttcgg cgagcaaagc ggagtcgtgc aggatccaag
     1021 ccacattgga cgtggccgga atgcgaggcg aggtgcggca ggtcctcgaa acatctgtgc
     1081 cggcccacgc ggtactcgac gcggccgacg aggtgggcgc ggagctggtc gtggccggcc
     1141 tgcggcggcg gactcggtgg ccaaactcat cctcggcagg ggcgccggac agtgctgctg
     1201 ggcgcgagct atccggtgct cgccgtcaag gccgaggcgg cgccgctgat cgagacccga
     1261 tcgtcgggta cgacgcgcgc cccgtccacg cagaccgtga ccaaggccgc gaaagccgtc
     1321 accaggacca agaccgcgac caagacgaca acgaagaccc ggacggccac caaaaccgtc
     1381 gccgccgcag cacctttcgc cgcaccggtg gtggcggcag tgtgtactac gccaactgcg
     1441 acgcagcccg cgcggccggt gccgccccgg tccggatcgg cgaccccggc taccgggccg
     1501 gcctcgaccg ggacggcgac ggcgtcggtg cgagaactgt ccagcgctgc ggaactggtc
     1561 agcgccgcaa ggagggatcg gcacggcata ccgtccggct cgtgttcagt cgccgaccac
     1621 acgtgaggat cgggcaattc cgtcgcccgg gacaggaatg catgctgtgg accagcgcgc
     1681 aggcatcgtc gccgaccggg atcgcgcagc gacgcgaggg cgacgccgtt gccctgtcga
     1741 cgggggtgag ttgcccgctc gataggacca aacgccctcc ctatcgacga gagggggagg
     1801 aaaccggggg acaaccaact cccaggttgc ggcgtcatac tgataggtgc gcccccggga
     1861 gggcgccgtg accgtcgtcg cctcacccca cgaaagccgc aatgccgacc cctcgcacgt
     1921 ccacgcctgc caccacgacg aaacccaaga agaagcgccg ctggctgcgg cgcacgctgt
     1981 ggaccctgtt caccctcggc ctgctcggcg ccatcggcct ggtcatcgcc tacatcgtga
     2041 cgccgatccc caagcccaac gaggcggcac tgcagcagtc ctccacgatc tactacagcg
     2101 acggcaagac cgtcatggac cggttcgccg aggtcaaccg gtccatcgtg cccatcgacc
     2161 ggatccccaa ggaggtccag gaggccttcc tcgccgccga ggaccgcagc ttctacgaca
     2221 acaacggcat ctcccccaag ggcatcgccc gctccatctg ggtgggcctg cgcggcgggc
     2281 cgcagcaggg tggctcgacg atcacgcagc aatacgtcaa gaactactac ctgacccagg
     2341 accgcaccct gaccaggaag ttcaaggaga tcatgatctc cgtcaagatc gacggtgagc
     2401 tctccaagga cgacatcctc gccaactacc tcaacacgat ctacttcggc cgcggcgcct
     2461 ccggcatcca gaccgcatcg caggcctact tcggcaagag cgtcaaccag ctcaccgtct
     2521 ccgagggcgc cctgctcgcc agcgtcatcc gcggcccgtc ctactacgac ccgcgcctgg
     2581 gggcggagca gaagcagaac gccaaggagc ggtggggcta cgtcctcgac ggcatggtcg
     2641 accagggctg gctgacccag gccgagcggg acaaggcgaa gttccccgcg acgatcaaga
     2701 tccgcaccaa ccgcatcgcc accggcccgg aaggcttcct ctccgagatg gtccgcgccg
     2761 agttgcagag caaggtcggc ctgaccgagg acgacctggc ccgcggcggc taccggatca
     2821 cgacgacgat cgacaagcgt gcccaggagg cggccaagtc cgccatcgcc gcccggatgc
     2881 cgcagggcgt caacgacctg cacgtcgggt tggccgcggt ggcccccggc gacggcgcga
     2941 tccgcgcgct gtatggcggt gccaaatacg gatccggcga cctcggctac ttcaacaccg
     3001 cgacccaggc cgcgatgcag gccggctcga cctacaaggt ctggacgctc gtcgcggcgc
     3061 tggagcgcgg cataccgctg tccacccgct tcgacgcccg ctcgccgatg ttcttcgagc
     3121 agttcaagag caccgagccg ggggcgacgc ccgcggcggc ggccggtgag gtggtcaact
     3181 tcggcaacca cggctacggc gtcgtcgaca tggcgacggc cacggggctg tcggccaaca
     3241 cctactacgc gcaactcaac atcgaggtcg gcccggagag cacccgtgac gccgccgaga
     3301 ccgccggcct caaggccgcg ggcgcccaga aggaactggg cgacaactac gccaacgtct
     3361 tcggcaccga cctggtcagc gtcctcggcc aggccaacgc ctacgcgacg atcgcggccg
     3421 aggggaggcg ggccacgccg tacatcatct ccggcgtgcg ggcgacgggg cagttcaaga
     3481 tcaactacaa ggccgacaag caggtcaaga ccgtcttcga caaggcggtc atgcgcgaca
     3541 ccatccacgc gatggcgcgg gtcacccagg ccggcggcac cggcgccgcc gcggccgccc
     3601 tcggccgtcc cgccgcgggc aagaccggca cgaccagcga caactactcc gcctggttcg
     3661 acgggttcac acccggccag ctcgccgccg ccgtcggcat ctacaagggc gacggctccc
     3721 tggtccccga gaaccagatg aacgccatac ccggcgtcgg tgagctcacc ggcggcaccg
     3781 tgccggtcaa tatctggacc gacttcatgc gactcgcgct cgagggccag ccgatcgcgc
     3841 cgctgccgcc ggccggcaac gtcagcggcg gcagcccgac gagcgtgaca accgcgccga
     3901 cgacacagtc gagcacgacc acgcagtcga gctcgtccag cagcagcagt tcgagcagcg
     3961 agagcgtgac ccagacccag accgagacga cgacgaccga gtcctcgacg cccccgccga
     4021 ccaccgacac gagcacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacga
     4141 cgccgccgcc ggcgcaggag acgggtctgc cgaccagctc ctcctcgggt ccgatacggc
     4201 cgacggacac caccagcgca ccgtaggctg cagccgtgcg ccccgacgtc ctgcctccgc
     4261 ggtcgaccgc gttcctcggt ggcccgcccg gacggtatgc ccgcgaacgc ggctggtggc
     4321 gcaccatcgc cacctggctg gtgccgctca cggccatacc gatggccttg gcggtgttgc
     4381 agcgcgagcg ctgcatccgc tacgggtgga ccgggagcga ccagttctgg cggatgtgct
     4441 tctccgacct gcccgcgcag tacgccatcg ccgggctgga cacgggcctg accgggtatc
     4501 tgcgcgacgc gaccctcgac caacccgtgc ttgccggctc agtgatgagc ctgctcggcg
     4561 ggctcgtccc ggacggcagc gcgatcgacc agtcgcgctc gtacttcctc tactgggtcg
     4621 ggctcatcac cctgctgctc gcggcgtgcg tctggtgcac cgtccacgtc gtgcccgacg
     4681 atctgcaggc cgcggcgcag gtcgcgctct cccctgtcat cgtgctcgct gcggtgttga
     4741 gcagcgacgc gctcgccgta gccctcgtcg ccggggcgat gctcgcctgg caccgcgaca
     4801 aggtggcgct gaccggcata ctgctgggcc tgggcatgat gacccgcggg tatgtcgccc
     4861 tggtcgccat cgccctcctc ctcgccgccc gccgcgaggg ccggctgcgg gccgcatggg
     4921 aggcggtgct cttcgccgcc gcgacggcgc tcttcgtcgc catcttcttc atcatcgccg
     4981 cgccggatgc gctgctcgcg ccctatctga actggtggga gggcgatgcg ggctacggct
     5041 cgctgtggct gatcccgtcg atcgccggtc acgccctgcc cgcctgggcg gtggcgttgc
     5101 tcgccgccgc ggggtgggtg ctggcggtcg tcctcggctg ggtcttcacc cgcgacgcct
     5161 atcgtgcgcc gacgtggccg caggtggcgc tggtcgtcgt ggggatgggg atgatgctgg
     5221 gcaagacggt gccggtgcag gccgcgttgt ggctgctgcc gatcgccgcg atggccggga
     5281 tgcggtggcg cgatcacctc atctgggccg gcgccgaggc gctgcacttc atggccgtat
     5341 ggctgtatgt cggcgcccag accacgccca accgcgccct gccgggggcc tggtatgtcg
     5401 tgttcctcct gatccgtacc gccggcatca cgtggctcgt gacgcgcgtc tggcaggccg
     5461 gcctcttccc gcccgcccgg cacgagcgcc acgatgacta ccaaccggta ggtaagacgg
     5521 ttgggcggtt ataaccgcca aggtgtctta cctacccgcg ggtcatgagt cgtatgccgt
     5581 gggccacctg atcgaggaag gcgccggggt ccaggcgtag cccgagcaca gggatgcgca
     5641 gcacgcgccg gttctggagg gttacggcgt tggcccgcag ggcgtcgtcg accggtgcga
     5701 gcccgaggct gtgctgcacc ccgtcgatct cgacgacaag tccgcactcg aaggcgacat
     5761 cgagatagat ccgccctttc ggcccgcgcc ggagctgttg gcgcaccggc tccgggagcc
     5821 cccgctcgcg gcagaagcgg gcgaagtcca gttcgccgag tgagtgcgcc ccatcgcaca
     5881 cgtccttgat caccgcatcg aggaagctgc ggcggggcga gcgccggacc gtggtccagc
     5941 gcgcgagcag gtcttcggcc cgcacgatcc gctgctggac cgggaggcag atcagcaagg
     6001 ccgcctgccg gtcgctaacg gccaattgtg cggcgcgcac ggtcgccgtc gccggctcga
     6061 cccgcggcag ccccggccgc agcggtccga ggacccgccg ctgggtgatg cggacccctg
     6121 ggtgcgggtg gatgcgcgcc cccaacggca cggagatctc gatgtggtcg aggacgaaac
     6181 ccgacatccc ctcggcgatc agggcgctcg cgccgtcgag ggccgcccgg gtgcccgatt
     6241 cccacacggc ccaccagaaa ggggcgaagg ccgtgtccac ggtggcgggg ccagcctttc
     6301 ggggcggcgc catgagggcg gtggccttca actgccgcgc ccgccggccg tctttggcca
     6361 tcccggtcac gccgaggggg aggtggtgcc cgtcatactt cttcccgtat cccagccagt
     6421 gcggcaccgc cgcggtgggg acggcaatcg tgttggggcc caccgtgatc agccgcccgg
     6481 catcgagatc ggcctcgagt tcggccttgg tgaagcccgc cccgagcaga tcggcacgcc
     6541 gggccagtcc acagtgccgg accgcgaaat ggtgggccac ggcggcgcgc cacggacccc
     6601 ggggcaggcc ctccgggggc ggcgagggga ccggtggtgg cggcatacgg gcagccaacc
     6661 cggtctggtc cgatctgtct ccgcccccgg ccacgtcctg tggacaagtt aggtaagacg
     6721 ctttggcggt tataaccgcc caggtgtctt acctaccggc gggtcactca tcgccagccg
     6781 cgacgagatg cggcacgcag gagagggagg cgaggatcgg caacacctga ccctcgcgcc
     6841 gcagcccact caccacgtgc ggcatcgtcg ccccggcggc cttggcgaac acccggcgcc
     6901 cgtcggccaa gtcgaggacg ccggcatacg acccagtgaa acccgagccc accggcggcc
     6961 ttgcgctcgc cactgacgcg ccggcaagcg ccgcgacggc ctcccggacg gcagccggca
     7021 gggcggcgaa ggcggggcgc gcggaggtgc ggtggtagtc gacgggggcg gacggcatac
     7081 cgccagccaa cccaggcgac ccaggcactc gcaagcggat aacgcgcacc tgtggacaac
     7141 cgattctgtg tatgccgagt cacccgatac ccttgacagg ttgcccccgc cccccgaggc
     7201 ggccgggcgg catgacaacc ctcctgtcac ggaccgaccg tgaccgctat cagaccaaag
     7261 gaggcgggtt ttcagcatgc gtcaatacga actcatggtc atcctcgacc ccgagctcga
     7321 cgaccgccag gtcgggccca ccatggagaa gttcctcggt gtcgtcaaga ccgacggcgg
     7381 aaccgtcgac aacatcgacg tgtggggccg gcgccgcctg gcgtacccca tcaagaagaa
     7441 ggacgaaggc agttacgtcg tcaccctgtt cacctccgag ccggcgacgg ccaaggagtt
     7501 ggaccgccag ctggggctct ccgagctggt catgcgcacc aagctgctgc gcgccgacgc
     7561 ctgatccacg actgactcga ggagccctgc catggccaac gagaccacca tcaccatcat
     7621 cggcaacctg accggtgacc ccgagttgcg tttcaccccc tccggcgctg ccgttgcgaa
     7681 cttcaccgtg gcctccacgc cgcgcacgtt cgatcgcaac agcaacgagt ggaaggacgg
     7741 cgagacgctg ttcatgcggt gctccgtatg gcgtgaggtc gccgagaatg tcgccgagtc
     7801 cctccaccgg ggcacccgcg tcatcgtctc ggggcggctg aagtcgcgct cgtgggatga
     7861 caaggagacc ggccagaagc gcaccgtcat cgagatggac gtcgacgagg tcggcccatc
     7921 cctgcgctat gccaccacgc aggtcaccaa gacccagcgc ggcggcggca acttcggtgg
     7981 tggcggcgac gagggcggct ggggccagca gtccggcggc ggccagtccg gcggcggcca
     8041 gcccggcggc ggccagggtg gccagggcgg gtggggccaa caggctccgc agtccggcaa
     8101 ccagggcggc ggctgggggc agcaggcccc ccagtccggc cagaacggtc aaggcggcca
     8161 gggcaactcc ggcggctggg gcggcggcgc cccgtcctac gacgagcccc cgttctgagt
     8221 cctatgggtt cgattccggt tcgcctcggg cgatctgcga gtcggcttat cgatagggag
     8281 cccggcctgc gccctcacca aatgaggact cacctgatct gcgctcccac cactaatcca
     8341 gtttgatcca tcccgagttc gaagctcggg ctcatccgaa aaggagagca ccacgatggc
     8401 taagcccgtt gtgcgtcggc ccaagaagaa ggccaacccc ctcaagtccg cgaagatcga
     8461 gaacattgac tacaaggacg ccgcgctgct gcgcaagttc atctccgacc gcggcaagat
     8521 ccgttcgcgc cgcgtgaccg gggtgtcggt gcaggagcag cgcctgatcg cccaggccgt
     8581 caagaacgcc cgcgagatgg cgctgctgcc ctacagcagc tcctcccgct gacccctgag
     8641 accgaaggag aacacctgat gaaggtcatc ctgacccacg aggtcaccgg cctcggcacc
     8701 gcgggtgacg tcgtcgacgt caagcccggt tacgcccgca acttcctcta caagcggggc
     8761 ctggccaccg cgtggagcgc cggcgcgcag aagcagatcg acgccatcgc ccgtggccgt
     8821 gaggcccgtg ccgtcaagtc cctcgaagag gcccagtcga tcaagggcaa cctcgaggcc
     8881 agcacggtcc aggtggccgc gcacgccggc aaggagggcc gcctgttcgg cgccgtctcc
     8941 accgccgaca tcgcggccgc cgtcaaggcc gcgggcggtc cggacctcga ccgccgcacg
     9001 atcgaggtgc cgaccccgat caaggccgtc ggcagccaca ccgccctggt gcgtctgcac
     9061 ccggaggtcc aggcgaacct gacgctggac gtcgtcgccg gctgaccgca tacgactgcg
     9121 cagcgccccc gcatccaccc ggatgcgggg gcgtgttgct ttgccgggca ggccaggccg
     9181 ggttcggtgc ggattcatcc atctcgttag atcgcaggtg ggcggcggtt ccc
//
LOCUS       DAOPJG010000137         9138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8001_length_9138_cov_6.099857, whole genome shotgun sequence.
ACCESSION   DAOPJG010000137 DAOPJG010000000
VERSION     DAOPJG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8001_length_9138_cov_6.099857"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKU75_11905"
     CDS             <1..456
                     /locus_tag="PKU75_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPF81629.1"
                     /translation="PALADSGHLDAAYDLLLQRAAPGWLAMLDRGATTMWERWEGIDE
                     DGMPHASLNHYSKGAVIGFLHTHVAGLRRTEPAWRRSRIEPRPGGGVTWARAHHDSPH
                     GRHTVEWSLRDNDFELTTEVPAGCRADLVLPGREPEDVGPGRHTRTCRV"
     gene            449..1651
                     /locus_tag="PKU75_11910"
     CDS             449..1651
                     /locus_tag="PKU75_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HPF81630.1"
                     /translation="MSDAHTPQDHPGRHIAIPLLLTLALLAAFSPMATDLYLPGFPDM
                     VGDLGTDASGVQLTLTTFMLGLSAGQLVWGPLSDRFGRLRPLLLSGLICVIAGALAAA
                     APSLGLLILARVVQGFTGAGGLVIGRAVVTDLTSGRDTARVMTLMMTVGGIAPILAPF
                     AGSLMIGSIGWRGLLWVLAGIGALSLAAVRLAIRESLPPERRLGASVPLRTAFGHVLR
                     ISEYRRQVAVFVSAFGVLMSYIAASPFVYQNIIGLSEIHYGMAFGANAVGMVTTGYAV
                     SHLVHRVGPRRVVLTSVLVQVAATLAFVVLVLTHAPVWTHAVAIFFAIAPMGGILGPT
                     AALVMAQARSSAGTGSALLGTSQHAFGAVVSPLVGLGGAGSALVPAAIMLTCSALALA
                     SVLRTAQD"
     gene            complement(1726..2526)
                     /locus_tag="PKU75_11915"
     CDS             complement(1726..2526)
                     /locus_tag="PKU75_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF81631.1"
                     /translation="MTSTDVTTPALDLSHIDLHYGYVRALADIDFHVMPGEVVGLLGD
                     NGAGKSTLLKVMSGAHRPSEGTIRVHGKEVSFHSPSDASEAGIQMVYQDLALVDAQDI
                     STNLNIGREILRKGPLGWLGFVDTKAMRKRAESELDRLGVRTAPMTRPTEMLSGGQRQ
                     VVAVARSAIRVSGDANGVLLLDEPTAALGYEQTKLVEGLIRRMADQGIAIVIVTHNLP
                     LCHEVADRVVILNRGRKVADVPMRGTDPDHIVGWITGARPSMFEDRAS"
     gene            complement(2532..3614)
                     /locus_tag="PKU75_11920"
     CDS             complement(2532..3614)
                     /locus_tag="PKU75_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF81632.1"
                     /translation="MSTPVSDSASRGDPSSAAAADTVVPTGHDMPRGVAALVFLRDRG
                     IFILWLLLILVFSFWCAPYFFTWSNAMLIANAAALTAIFAAGVGLGVMSGVLDLSLPG
                     TAAFCACLTGWLAVNKGVSLWIAVPIALVCGALVGVVNGLISLQGFNPIIVTIGTLSV
                     LTGLASVITGGLTIPGLDGLTFMGTRRYFGVPAPVYIVAALYLVGTIFLTRTRDGIRF
                     MAVGGNAEAVRRSGIHSDRYKVMGFVISGICAAIGGLVATAITTEANPDASPAIIFSA
                     LTAVALAGVAMSGGRGSLPRVLVGALILATIANGLIIRGVQTYWATVITGVLLLASLG
                     LERFIQKSVSDRLMATSNLSVHQKKV"
     gene            complement(3618..4637)
                     /locus_tag="PKU75_11925"
     CDS             complement(3618..4637)
                     /locus_tag="PKU75_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPF81633.1"
                     /translation="MSHSTAGRVGRMSPAIAAAVGAVSLLAACGGTTQPAGGDSSAAG
                     SSSGSAASAAGGKTIVFSPLALKIPAMKGLSEGVKAYGEGKGFTVDIQDPNLDPQKQI
                     QQLTAVIESGKASGVWMIAVQPTALKSVVQLAIDKKVPMVVNGVPADYGFDALQPGIT
                     FDTIDYTAQGKAIGEQLAKCINEKLSGKAKVLFVDATTGTAGKKEIEEGAMAALTAGA
                     PGAEVVTHVEVTDRAATQTKIGNALQGNPDVTAVQGMNDEGALAALGAFAAAGKELPC
                     VTEAGGNDEVLKAVKDGKIYASVALQFQDDMAQSFDTLVGLIADPTQTGKQLTVPQKI
                     YTKDS"
     gene            4824..5753
                     /locus_tag="PKU75_11930"
     CDS             4824..5753
                     /locus_tag="PKU75_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF81634.1"
                     /translation="MTWAERDTYVEEVTRDVFAFIQPDGSWMVNNCGFVVGSSGRSVL
                     VDTSSTERRTAQFMDAVRAHSSQPPMALVNTHHHPDHTYGNFLAPQGTPVVGHVRCRE
                     EVLAAGLEATKVITTPDYGNLELRPPDFTFTDRLTLHLDETPVELEYVGPAHTSNDVL
                     VWLPEQKVVFAGDLAFAGGQPFLLEGSLAGFPKALARIKELGAEALVPGHGPVVRGAD
                     VNTLLDDLTGYVEFVSRIASEGMADGRTPLQVAEKYKDNPYSGWQETERLVGNLHRAY
                     SELSGNDLFTRLRVPDVWPEMVSFHGGPIGCKA"
     gene            5855..7504
                     /locus_tag="PKU75_11935"
     CDS             5855..7504
                     /locus_tag="PKU75_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPF81635.1"
                     /translation="MPASLHIHALAASFGARPIFSGLDLTLGPGDVTAVVGPNGAGKS
                     TLLRIIAGEHPADTGTVRLAPPDASIGYLPQSPPRPEESIHEYAARRAGVTAAQGEFD
                     SATAALADGAPGAEERYAVALERWMALGAADLDARLGVSLARVGLDVALDRPLGRLSG
                     GQAARAALASILVSQQEVLLLDEPTNNLDASGLAVLTAYVTSLAGPVLIASHDRRFLD
                     EVATSVLELDIHQQTVTRFTGGWSDYRTAKDLARDQADEAYAAYTLQRDALVERARRQ
                     SDWAAKGRAGKADLGPHDGIGKKYIEDKAKRMDQRAARARAAAERLPEVEQRRKEWEL
                     RYTIAEAPPSAEVVITLDQVSVERGAFRIGPFSTTVARGERIAIVGDNGSGKSTLLHA
                     LLGEVPVASGRISWGTRVHTGTLDQARGVIAGPDDLLDAVTAALGSTDRADVRTLLAK
                     FGLGPEMVERECDSLSLGERTRAAMAVLQGRAVNTLILDEPTNHLDVPAIEQIESALA
                     AFGGTLLIVTHDRTLLDAVAPTTRWEVSRNGEVASVRVSMW"
     gene            complement(7510..8079)
                     /locus_tag="PKU75_11940"
     CDS             complement(7510..8079)
                     /locus_tag="PKU75_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81636.1"
                     /translation="MALSPCLDGTAAEWIVRDKRPWTRVAGFGPGGFSAYARLRFLPD
                     PGSPGDREADAPEHELTDLEEMARAAEVLAPHTTSSNRYFGVWDGWGGAFDSPALPRF
                     AIRNRVYYLLSGVPEDLWTFGTSGMATYWPWPAFVWPEDRAWCLAHDVDPHWAGIGAS
                     KEGIKALLADDLLDVVKADPRVPQPFYRG"
     gene            complement(8089..8808)
                     /locus_tag="PKU75_11945"
     CDS             complement(8089..8808)
                     /locus_tag="PKU75_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPF81637.1"
                     /translation="MTWTVLLLLGLAIAAGAFIQSAIGFGINVVAGPIVLLAAPDLMP
                     GALVLGGFILPLIQIAREGLDVAWPPLGWSVLARALTTPIGVWLVLVLSPSQIGIVLG
                     AVILLTVALSIWSLDIRATVPNALVAGALSGISGASAAVGGPFTAILFQHEPPARMRS
                     TLAVSFLLGSALSTIGLAQAGALGWSDLRAALVWAPFMVVGYAASGRLRRRLAESGMR
                     TAVLAFCVIAACVIVIRALAA"
     gene            complement(8824..>9138)
                     /locus_tag="PKU75_11950"
     CDS             complement(8824..>9138)
                     /locus_tag="PKU75_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF81638.1"
                     /translation="EVTYTHEFAPTVNDPGCADVAAAAARAVVGPDAVEADCEPIMPS
                     EDFGVFGRHTSACFILIGNGASGEIGGTPLHSSDYDFNDAIMPTGSQVLAEIVRRELP
                     EA"
BASE COUNT         1496 a         3102 c         3131 g         1409 t
ORIGIN      
        1 cccgctctcg ccgacagcgg gcacctcgac gcggcatacg acctgctcct gcaacgcgcc
       61 gccccgggat ggctggcgat gctcgaccgt ggcgcgacca cgatgtggga gcgttgggaa
      121 ggcatcgacg aggacggcat gccgcacgcg tcgctcaacc actacagcaa gggcgccgtc
      181 atcggattcc tgcacaccca cgtcgcgggc ctgcggcgga ccgagccggc gtggcgccgc
      241 tcgcgcatcg aaccgcggcc tggtggcgga gtcacctggg cccgggccca tcacgattcg
      301 ccgcacggac ggcatacggt cgaatggtcg ttgcgggaca atgatttcga actcaccacc
      361 gaggtgccgg caggatgccg ggccgacctt gtcctccccg gacgtgagcc ggaggacgtc
      421 ggtcccgggc ggcataccag gacctgccgt gtctgacgct cacaccccgc aggatcaccc
      481 cggccgccac atcgcgatcc ccctgttgct gaccctggcg ctgttggcgg cgttctcccc
      541 gatggcgacc gatctctatc tgcccggttt cccggacatg gtcggcgacc tcgggacgga
      601 tgcgagcggc gtgcaactga cgctgacgac gttcatgctc gggctgtccg ccgggcagct
      661 ggtgtggggt ccgttgtcgg atcgcttcgg gcgactccgt ccgctgcttc tcagcgggct
      721 gatctgtgtc atcgccggcg cgctggcggc cgccgcaccc agcctgggcc tgctcatcct
      781 cgcgcgtgtc gtccagggct tcaccggagc cggcggcctg gtcatcggtc gcgccgtcgt
      841 gaccgacctg acgagtggcc gggacaccgc gcgcgtcatg acgctgatga tgacagtcgg
      901 cggtatcgcc ccgatcctcg cgcccttcgc gggcagcctg atgatcggct cgatcggctg
      961 gcggggcctg ctgtgggtgc tcgccggcat cggtgcgctc agtctcgcgg cggtgcggct
     1021 cgcgatccgc gagagcctgc cgcccgagcg gcggctcggt gcctcagtgc cgctacgcac
     1081 cgccttcggt cacgtgctca ggatcagcga ataccggcgc caggtcgcgg ttttcgtcag
     1141 cgcgttcggg gtcttgatgt cctacatcgc cgcctcgccg ttcgtctatc agaacatcat
     1201 cgggctcagc gagatccatt acgggatggc gttcggcgcc aacgccgtcg gcatggtcac
     1261 caccgggtat gccgtcagtc acctcgtcca tcgcgtcggc ccgcggcggg tggtcctgac
     1321 gtcggtgctg gtgcaggtgg ccgcgacgct cgccttcgtg gtgctggtgc tcacgcacgc
     1381 acccgtgtgg acgcatgccg tcgccatctt cttcgccatc gcacccatgg gcggcatcct
     1441 cggacccacg gcggcgctgg tcatggcgca agcccggtcc tcggccggca ccggaagcgc
     1501 gctgctgggg accagccagc acgccttcgg cgcggtcgtc tcgccgctcg tcggcctcgg
     1561 cggggccggc tccgccctgg tccccgccgc catcatgctc acctgctcgg ccttggccct
     1621 ggccagcgtg ctgcgcacgg cccaggactg acaaccacag gcgcggcgaa aaaggtaaga
     1681 cggttgggcg gttataaccg cccaaccgtc ttacctactc gcgggtcatg atgcgcggtc
     1741 ctcgaacatc gagggccggg cgccggtgat ccagccgacg atgtggtcgg ggtcggtgcc
     1801 ccgcatcggg acgtcggcga ccttgcgccc ccggttgagg atgacgacgc ggtcggcgac
     1861 ctcgtggcac agcgggaggt tgtgcgtgac gatgacgatg gcgatgccct gatcggccat
     1921 ccggcggatc aggccctcga cgagcttggt ctgctcgtaa cccagagcag cggtgggttc
     1981 gtcgagcagg agcacaccgt tggcgtcgcc ggagacccgg atcgcgctgc gcgccaccgc
     2041 aaccacctga cgctggcccc cggagagcat ctccgtaggc cgggtcatcg gggcggtgcg
     2101 cacgccgagg cgatccagct cggactcggc acgcttgcgc atggccttgg tgtccacgaa
     2161 ccccagccaa ccgagcggac ccttgcgcag aatctcccgg ccgatgttga ggttggtcga
     2221 aatgtcttgc gcgtcgacga gagccaggtc ctgatagacc atctggatgc ccgcttcgga
     2281 ggcgtcgctc ggggagtgga aggagacctc cttgccgtgg acgcggatgg tgccctcgct
     2341 gggccggtgc gccccggaca tcaccttcag cagggtcgac ttgccggctc cgttgtcgcc
     2401 gagcagtccg acgacctcgc cgggcatgac gtggaagtcg atgtccgcca gggcacgcac
     2461 ataaccgtaa tgcaggtcga tgtgggacag gtcgagggca ggtgtggtca cgtcggtgga
     2521 tgtcatcgtg atcagacctt cttctggtgg accgacagat tggaggtggc catcagccgg
     2581 tcggagacgc tcttttggat aaagcgctcc aggcccagcg aggcgagcag cagcacaccg
     2641 gtgatgaccg ttgcccaata ggtctggacg ccccggatga tcaggccgtt ggcgatcgtg
     2701 gccaggatga gcgcaccgac aagcacgcgg ggcagcgagc cacgaccccc gctcatggcc
     2761 accccagcga gcgccaccgc tgtcagggcg ctgaagatga tggcggggct ggcatccgga
     2821 ttggcctcgg tcgtgatcgc cgtggcgacg agcccgccga tggcggcgca gatgccggag
     2881 atgacgaagc ccatcacctt gtagcgatcg ctgtggatac cgctgcgccg caccgcttcc
     2941 gcattgccac caacggccat gaaccgaatg ccgtctcggg tgcgggtcaa gaagatcgtg
     3001 cccacgagat agagagcggc gacaatgtag acgggtgcgg gaaccccgaa atagcggcga
     3061 gtccccatga aagtgagccc gtcgaggccg gggatcgtca gaccgccggt gatgaccgac
     3121 gccagcccgg tcagcaccga caaggtgccg atcgtgacga tgatcgggtt gaagccctgg
     3181 agggagatca gcccgttgac gacaccgacc aaggcaccgc ataccaacgc gatcggcacc
     3241 gcgatccaga gggagacccc cttgttgacg gccagccagc ctgtcagaca ggcacagaac
     3301 gcggccgtgc ccggcaacga caaatcgaga actcccgaca tgacgcccag cccgacgccg
     3361 gccgcgaaga tcgctgtcag cgccgcggcg ttcgcgatga gcatggcgtt gctccacgtg
     3421 aagaaatacg gagcgcacca gaacgagaag acgaggatca gcagcaacca caggatgaag
     3481 atgcctcggt cgcgtagaaa gacaagtgct gcaacacctc tcggcatgtc atggccggtc
     3541 gggacgaccg tgtcggccgc cgcggcagaa gacgggtctc cccgcgacgc cgagtcactg
     3601 acaggagtgc tcatggctca gctgtccttg gtgtagatct tctgggggac ggtcagctgc
     3661 ttgccggtct gggtcgggtc ggcgatcagg cccacgagag tgtcgaagga ctgggccatg
     3721 tcatcctgga actgcagggc gaccgaggcg tagatcttgc cgtccttgac ggccttgagt
     3781 acctcgtcgt tgccgccggc ctcggtgaca cacggcagct ctttgcccgc cgcagcgaac
     3841 gcaccgagcg ccgcgagcgc accttcgtcg ttcatgccct ggacggccgt gacatccggg
     3901 ttgccctgca gggcgttgcc gatcttggtc tgggtcgcag cccggtcggt gacctccacg
     3961 tgcgtgacga cctccgcgcc cggagcacca gccgtcagcg cggccatagc gccctcctcg
     4021 atctccttct tgccggcggt gccggtcgtc gcgtcgacga agaggacctt ggccttgccg
     4081 gagagcttct cgttgatgca cttggccagc tgctcaccga tcgccttgcc ctgtgcggtg
     4141 tagtcgatgg tgtcgaaggt gatgcccggc tgcagggcgt cgaacccata gtcggcgggg
     4201 acgccgttga cgaccatcgg gaccttcttg tcgatcgcca gctggacgac ggacttcagg
     4261 gcggtcggct ggacggcgat catccacacg cccgaggcct tgccggactc gatgacggcg
     4321 gtgagctgct ggatctgctt ctgggggtcg aggttcgggt cctggatgtc gacggtgaac
     4381 cctttgcctt cgccataggc cttcacgccc tcggacaggc ctttcatcgc ggggatcttc
     4441 agcgcgagcg gggagaagac gatggttttc ccgccggcgg cggaggccgc ggatccggac
     4501 gaactcccag ccgccgagga atccccaccc gcaggttggg tggtcccgcc gcaggcggcg
     4561 agcagagaga ccgcaccgac tgcagcggcg atcgcgggcg acatgcggcc cacgcggccg
     4621 gcggtcgagt gtgacatgag catctcctca ttgagcggtg ccgacggacg tcgccggcgt
     4681 ctgacgagta ggagagcacg cggcatacga ccctcggaag caacgggcgg gaatgcgagc
     4741 catcacgcgc agccatacct agccctctcc ccgcgcgcga ggactggtgc ttgactcgct
     4801 atccccacac gaaggagcga gcaatgacct gggccgagcg cgacacctat gtcgaggaag
     4861 tgacccgcga cgtcttcgcc ttcatccagc ccgacgggtc ctggatggtc aacaactgtg
     4921 ggttcgtcgt gggctccagc gggcgcagcg tcctggtcga cacctcctcg acggagcggc
     4981 gcaccgccca gttcatggac gccgtgcgcg cccactcgag ccagccaccg atggctctgg
     5041 tcaacaccca ccaccatccc gaccacacct acggcaattt ccttgcgccc caggggactc
     5101 cggtcgtcgg tcacgtgcgc tgccgcgagg aagtgctcgc cgccgggttg gaggcgacga
     5161 aggtcatcac gaccccggac tacggcaacc tcgaactgcg gccgccggat ttcaccttca
     5221 ccgaccgcct gaccctgcac ctcgacgaga cgccggtcga gttggagtac gtcggtccgg
     5281 cccacacgag caatgacgtc ctggtatggc tgccggagca gaaggtcgtc ttcgccgggg
     5341 atctcgcgtt cgccggcggg cagccgttcc tgctcgaggg atcgctggcc ggcttcccga
     5401 aagcgttggc gcgcatcaag gaattgggcg ccgaagcgct cgtgcccggc cacggtccgg
     5461 tggtgcgcgg cgcggacgta aacactctcc tcgacgacct gaccggctat gtcgagttcg
     5521 tctcgcgcat cgcgagcgag ggcatggcgg acggtaggac gccgctgcag gtcgccgaga
     5581 agtacaagga caatccctac tccggctggc aggagaccga gcgcctcgtc gggaatctgc
     5641 accgcgccta ctccgagttg tcgggcaacg acctcttcac ccggctgcgg gtgccggacg
     5701 tatggccgga gatggtctcc ttccacggtg gtccgatcgg ctgcaaagcc tgaggccgtg
     5761 aactgacttg acccggcggc ggcccgtcgg gcaccctggc ggcatgtgcg actgacctct
     5821 tttcccttgc ccatcaagcg atcagaggtc tcccatgccc gcatccctgc acatccatgc
     5881 gctcgccgcc agcttcggtg cccgccccat cttctccgga ctcgacctga ctctcgggcc
     5941 gggtgacgtc accgcggtcg tcggccccaa cggcgccggg aagtcgacac tgctgcggat
     6001 cattgcgggt gagcatccgg ccgacaccgg caccgtccgg ctcgccccgc cggatgcgag
     6061 catcggctac ctcccccagt cgcctccccg ccccgaggag tcgatccacg agtatgccgc
     6121 ccgccgcgcc ggcgtcaccg ctgctcaggg cgaattcgac tccgccacag cggcgttggc
     6181 tgatggtgcg ccaggagcag aggagcggta tgccgtggct ctggagcgct ggatggcgct
     6241 gggtgccgct gatctcgacg ctcgtctcgg ggtgtcgttg gcgcgcgtcg gtctggacgt
     6301 cgcgttggat cgtcccctcg gccggctctc cggtggccag gcggcgcgcg cagcgctggc
     6361 ctcgatcctg gtgagccaac aggaggtgct gctgctcgac gagccgacca acaacctcga
     6421 cgcgagcggt cttgccgtcc tcaccgcata cgtcacgagc ctcgcgggac ctgtcctcat
     6481 cgcctctcat gaccgtcgct tcctcgacga ggtggccacc agcgtcctcg aactcgacat
     6541 ccaccagcaa acggtcaccc gcttcaccgg cggctggtcg gactaccgga cggccaagga
     6601 tctggcgcgc gatcaggctg atgaggcgta tgcggcttac accctgcagc gcgacgccct
     6661 cgtcgagcga gcgagacgac agagtgactg ggcggccaag ggccgtgccg gcaaggccga
     6721 cctcggaccg cacgacggga tcggcaagaa gtacatcgag gacaaggcga agcggatgga
     6781 tcagcgcgcg gcccgagccc gggcggcggc cgagcgattg cccgaggtgg agcaacggcg
     6841 caaagaatgg gagctgcgct acacgatcgc cgaggcgccg ccgtcggcgg aggtcgtgat
     6901 caccctcgac caggtgtccg tcgagcgcgg tgccttccgg atcggaccgt tctcgacgac
     6961 agtcgcccgc ggggagcgca tcgcgatcgt cggcgacaac ggaagcggca agtcgaccct
     7021 gctgcacgcg ctcctcgggg aggtgccggt cgcgagcggc cgcatctcat gggggacgcg
     7081 cgtccatacc ggcacgctcg atcaggcgcg gggagtgatc gccggccccg acgacctgct
     7141 cgatgccgtc accgcggcgc tcggcagcac cgaccgcgcc gacgtgcgca cactcctcgc
     7201 gaagttcgga cttgggccag aaatggttga gcgcgaatgt gattcgctgt cgctgggtga
     7261 acgcacccgg gccgccatgg cggtcctgca gggccgcgcc gtcaacacgc tcatcctgga
     7321 cgaaccgacc aaccacctcg acgtgccggc catcgagcag atcgaatcgg cgctcgccgc
     7381 cttcggtggg acgctcctga tcgtcacgca cgaccggacc ctgctggacg ccgtggcgcc
     7441 gacgacccgg tgggaggtct cccgcaatgg cgaggtggcg tcggtgcgag tgtcgatgtg
     7501 gtgacggcgt cagccgcgat agaagggctg aggaacgcgt gggtcggcct tgacgacatc
     7561 gagtaggtca tccgccagca gggccttgat gccctccttg ctggcgccga tgccggccca
     7621 gtgtgggtcg acgtcatgag cgaggcacca cgcgcgatcc tccggccaga cgaacgccgg
     7681 ccatggccaa taggtcgcca tgccacttgt gccgaacgtc cacaagtcct ccggcactcc
     7741 cgacaggagg tagtagacgc gattgcggat ggcgaaccgg ggcagggcgg gcgagtcgaa
     7801 agccccgccc caaccgtccc agacgccgaa gtagcggttg gacgaggtgg tatgcggcgc
     7861 aaggacttcc gccgctcggg ccatctcctc gaggtcggtc agctcatgct ccggcgcgtc
     7921 ggcctcccgg tcaccgggcg accccgggtc gggcaggaag cgcaaccggg cgtacgcgga
     7981 gaagcctcct gggccgaaac cagcgaccct cgtccacggc cgcttgtcgc ggacgatcca
     8041 ttcagctgcg gtgccgtcga ggcacggcga aagtgccaca accaaacgct acgccgccag
     8101 cgcgcggatg actatgacgc aggccgcaat gacgcagaac gccaggaccg ccgtgcgcat
     8161 ccccgactcc gcgagccggc gacgcaggcg accggaggcg gcatacccga ccaccatgaa
     8221 cggagcccag acgagtgcgg cccgcaggtc cgaccagccg agggcgccgg cctgcgcgag
     8281 cccgatcgtc gacagggcgc tgccgagcag gaaggagacg gcgagcgtgg agcgcatgcg
     8341 ggcgggcggc tcgtgctgga agaggatggc ggtgaacggg ccaccgaccg cggccgaggc
     8401 acccgagatc cccgacagcg cgccggcgac cagcgcgttg gggaccgttg cgcggatgtc
     8461 caggctccag atcgacagcg cgacagtgag caggatgacg gcaccgagca cgatgccgat
     8521 ctggctgggg gacaggacaa gaaccagcca gacaccgatg ggtgtcgtga gggcacgagc
     8581 tagcacggac cacccgagcg gcggccaggc gacgtccagc ccctcacgtg cgatctggat
     8641 cagcggcagg atgaagccgc cgagcacgag cgcgccgggc atgaggtccg gagctgccag
     8701 caggacgatc ggtccggcga cgacgttgat cccgaatcca atggcggact ggatgaaggc
     8761 gcctgcggcg atggccaggc cgagcaggag caggacggtc caggtcatca cgtcggtccg
     8821 ggctcacgcc tcgggcagtt cgcgccggac gatctcggcg agcacctgcg agccggtggg
     8881 catgatggca tcgttgaaat cgtagtcact gctgtgcagc ggtgtgccgc cgatctcgcc
     8941 gctcgctcca ttgccgatga ggatgaagca cgcggacgta tgccgcccga acacgccgaa
     9001 gtcctcgctc ggcatgatcg gctcgcagtc cgcctcgaca gcgtccggcc ccacgaccgc
     9061 ccgggcggcg gcagcggcca cgtcggcaca gccgggatcg ttgacggtcg gggcgaactc
     9121 atgcgtgtag gtcacctc
//
LOCUS       DAOPJG010000138         9127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8013_length_9127_cov_7.602954, whole genome shotgun sequence.
ACCESSION   DAOPJG010000138 DAOPJG010000000
VERSION     DAOPJG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8013_length_9127_cov_7.602954"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKU75_11955"
     CDS             complement(<1..534)
                     /locus_tag="PKU75_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF81639.1"
                     /translation="MSRIVLLAGKATGGIGVHVRDLSVGLRDLGHDVRVVTDRLTATT
                     FGLADALLLWPDLRRPDPRAVARLRSILVAADVVHAQGHQATILAGTLLAGVSRTRRP
                     RFAASLHNEFPSAASRSGRAALALERRALRRADLVTGASSDLVTAARTLGARRARLAE
                     VSSPRVLDLLAAGPRERA"
     gene            complement(531..2165)
                     /locus_tag="PKU75_11960"
     CDS             complement(531..2165)
                     /locus_tag="PKU75_11960"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPF81640.1"
                     /translation="MSVGANARLIAVLTAAARLAGFVRMLVFAACVGTTTVGTAYQSA
                     NTVPNVVFEIAAGGVLAAIVVPLVAARLGRGERAEADAIASALLTWAAAVLLPLVLIV
                     AALAPLISRWLLDSAAAVELGTRMLWVFAPQIVLYGIGIVLSGVLNAHERFAAAAVAP
                     LVSSLVVIATYVAYWLVLRGGSPSAAVWVLAGGTSLGVLALVATLVPTARRLGIRLHP
                     TLRFPPGVAAAARRLAGAGLIALLAQQISVLAVLWLANNRAAVGTINGYQYVQAVYLL
                     PYAVLAVPLATAAYPVLAAAEGQGAAAATTVAHTARRIVVLGGLAGAVLIAAADDVGA
                     FFGAIDAGRRGVGAQALAALPATLRAFAPGLIGFGLAALLTRALYVRGSARRGALVMA
                     GGWLVAALLPLILVGGAHDTARTLRLLGVGSSIGMTLAGLGLLALTRRSWGSAALAGV
                     PRALPAAIMAALFAASIGRALRASVPAADSAVTAVLAAGLIGGCTALVYLLWLWVFDG
                     ATLRLLLPGRPARIDDPADEDAEVTQPERTPGEGGR"
     gene            complement(2185..3000)
                     /locus_tag="PKU75_11965"
     CDS             complement(2185..3000)
                     /locus_tag="PKU75_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81641.1"
                     /translation="MTGAVAGAATAVLTRLLRTRPPGGGARWTRTNHAGRPISLLEGP
                     GYVAGSALGLAAAALASPRRQTGMGSVAAGLVACVGAGAAGLLDDLAGDSASKGLRGH
                     LGALRQGRVTTGALKIAGIGASGLVGAALVDRDAGRRLGPATLVGGAVIAGSANLANL
                     LDLRPGRALKATVLGGIPLALLGESAAASAVGAALGALPADLRGETMLGDTGANAAGA
                     LVGTALVARSGPLGRGVALAVLTALTLASERVSFTRVIESTPVLRDLDAWGRP"
     gene            complement(3027..3995)
                     /locus_tag="PKU75_11970"
     CDS             complement(3027..3995)
                     /locus_tag="PKU75_11970"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HPF81642.1"
                     /translation="MIDFRYHLVSLASVLIALAVGIVLGAGPLKEGISDSLDQQVAQL
                     RADKDALRSQLDASEKSVQEQEDFANAQLPAIVSGRLAERSVVVVTLPGAPDSLVVET
                     EDTLSAAGASVSGPVRIEQAWSDSSQEATTRREQLASTLAPTLGLTVDTEDAPDLGDV
                     LGALLAAPATPDGTTAPEAPSAVARAAAWTRLVSDGYVSGSLPATRANLVTVVGATQV
                     ATAQGSSGTTDPLPALAELAAALDDASDGVVIAAAIDPTAADSTSIIAAARARAAVRR
                     EVSSVDDASDPIGQASIVAALVEQEAGRAGQYGRAAGAKSPFPSTN"
     gene            complement(3992..5191)
                     /gene="steA"
                     /locus_tag="PKU75_11975"
     CDS             complement(3992..5191)
                     /gene="steA"
                     /locus_tag="PKU75_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HPF81643.1"
                     /translation="MAVSLSRRSREPVPAGATGMARVDRRTKNLTKRLQPGEIAVIDH
                     EDLDRVSAEALVACRPAAVVNAARSISGRYPNVGPTILLDAGIPLIDDAGPDAMTTIR
                     EGQWLRLDHGQVFAADAVADEAPALVAGVVQDDDSVADALSLARQGLSGQLQAFAQNT
                     MDYLVREKDLLLDGVGVPDIATKIDGRHALIVVRGYHYKEDLQALQHYMREFKPVLIG
                     VDGGADALLEAKRKPDLIVGDMDSVSDKALTCGAEIVVHAYRDGRAPGAERVRGLGVE
                     PVIFPATGTSEDVAMLLADDKGASLIVAVGTHATLVEFLDKGRAGMASTFLTRLRVGS
                     KLIDAKGVNRLYRSRISGVSMLVMLLVGMLVLGLALWATPGGSILLQLLGARWDDLWA
                     AIVGIFT"
     gene            complement(5197..6912)
                     /gene="recN"
                     /locus_tag="PKU75_11980"
     CDS             complement(5197..6912)
                     /gene="recN"
                     /locus_tag="PKU75_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPF81644.1"
                     /translation="MISEIRIGSLGVIQAAHLPLHAGLNVVTGETGAGKTMVVTGLGL
                     LFGARGDAALVRAPAPSLVVEGLIDLPADHPALARAADAGADVEDGLILVRTVAAGGR
                     SRAFAGGRSVPVGTLGELAENLVAVHGQADQWRLRSGEQHRQLLDEFGGDELAAALTH
                     YRDVHTQCRDLAAQVTRLEELVATQTADLDLLRAGVERIEAVDPQPGEDEALRAEAER
                     LAHGEQLRAAGASAAAYLLGDESAPDATGASEHIASAGALIDGVVEHDPALPELAGRL
                     REVGVLVSELGADLFAYADGIDVDDARLAAVHERRAELAGLAKAHGGSLESALTWLAQ
                     ASARLLDVDTAADDLPELREQLAAAEITRAAAARTVSDLRHAAGTRLAGAVTDELAHL
                     AMGSATLEVAVTGGDGEVRYGKDGVDEVEFLLRSGSGMPPRPIAKSASGGELSRVMLA
                     LEVVLGTSATSVPTYVFDEVDAGVGGRAAIDIGARLARLAEHAQVVVVTHLPQVAAFA
                     DQHLVVTKTDDGSVSQSDVRVVEARDREREIARMMAGTDSAAAVRHARELLADARARR
                     TACDD"
     gene            complement(6914..7831)
                     /locus_tag="PKU75_11985"
     CDS             complement(6914..7831)
                     /locus_tag="PKU75_11985"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPF81645.1"
                     /translation="MSSAHRRILLVAHPNRPDAIHLAREVAQRLLGEGIGIVLDPMTA
                     QAVDPGHDLVSIGDGDRPAEGCELVLVLGGDGSILRGAELSRGSDAPLLGINLGHVGF
                     LAESEREDTDATIERILDRDYAITERMTLDVVSRLDGREFARTWALNEATVEKASRER
                     MLELTVEIDGRPLSTWGCDGVVMATPTGSTAYAFSAGGPVVWPDVDAMLVVPISAHAL
                     FARPLVLSPRSRLAVEVVSGNEGQGVLWCDGRRAVDLPAGARVEVCRSAEPVRLARLS
                     EQPFTDRLVEKFDLSVHGWRGPRPNRAGP"
     gene            complement(7828..8640)
                     /locus_tag="PKU75_11990"
     CDS             complement(7828..8640)
                     /locus_tag="PKU75_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPF81646.1"
                     /translation="MSGGERLDQALVARGLARSRGQARELLEAGRVRLNEAPAAKPAT
                     RVGPGDRLAIDGDLNIRVGRGADKLDAAVTAFEPAGLAVAGKRALDVGASTGGFTQIL
                     LERGASEVLALDVGHDQLAEPVRSDPRVHDLSGRSIRGLTPDDIGGSVDLLVADLSFI
                     SLTLVMADLERVSGEAADLILLIKPQFEVGRGRLDKKGIVRDPTDRAGAMRDVLQAGV
                     DAGLCVRGLIASPVVGTHGNREYLGWLAKGGNVSASWQALEEQIDDLVRRDP"
     gene            complement(8637..8948)
                     /locus_tag="PKU75_11995"
     CDS             complement(8637..8948)
                     /locus_tag="PKU75_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81647.1"
                     /translation="MSEPTQLPDASDEAFHSQGIETEPASTEPASTEPANTEPASTEP
                     ANTEPDRTEEASPPHTGDLVIDSALRDLAETSGADLDAVLEQGSALEGVLRARLGDLD
                     G"
BASE COUNT         1242 a         3299 c         3434 g         1152 t
ORIGIN      
        1 tgcccgttct cgggggccgg ccgcgagcag gtccagcacc cgcggggagg agacctcggc
       61 gagccgggcg cgtcgcgccc cgagcgtccg ggctgcggtc acgaggtcgc tgctcgcccc
      121 cgtcaccagg tcggcacgcc gcagcgcacg ccgttcgagc gcgagcgctg cccgaccgga
      181 ccggcttgcc gcggagggga attcgttatg gagactcgcg gcgaaccggg gcctgcgggt
      241 ccgggacacg ccggccagca gggtcccggc gaggatcgtg gcctgatgcc cctgagcgtg
      301 cacgacgtcc gcggccacga ggatcgagcg cagccgagcc acggctcgcg gatccgggcg
      361 ccgcaggtcg ggccacagca ggagggcatc cgccagcccg aacgtcgtgg ccgtgagccg
      421 gtcggtcacc acgcgcacgt catggcccag gtcgcgcagc cccaccgaca ggtcgcgcac
      481 gtgcacaccg atgccgccgg tcgccttgcc ggcgagcagg acgatgcggc tcatcggcca
      541 ccctcccccg gcgtccgctc cggctgcgtc acttcggcgt cctcgtccgc cgggtcgtcg
      601 atccgggccg gccggccggg cagcagcagc cggagggtgg caccgtcgaa cacccagagc
      661 cacagcaggt agaccagggc ggtgcaccca ccgatcaggc ccgcggccag caccgcggtg
      721 actgcgctgt cggcagccgg gaccgaggcg cgcagggcgc ggccgatgct cgccgcgaac
      781 agggcggcca tgatcgcggc cggcaacgcc cgcggcacgc cggcgagcgc ggcggagccc
      841 cacgagcggc gggtgagggc cagcagaccc aggccggcga gcgtcatgcc gatgctcgac
      901 ccgacaccga gcaaccgcag ggtgcgtgcg gtgtcgtggg cgccgccgac gaggatcagc
      961 ggcagcagcg cggcgacgag ccagccgccc gccatgacga gggcgccccg gcgggccgag
     1021 ccacggacat acagcgcccg cgtcagcagc gcggccagcc cgaacccgat caaccccggc
     1081 gcgaaggcac gcagggtggc gggcagcgcg gccagggcct gcgcccccac gccccggcgg
     1141 ccggcgtcga tcgccccgaa gaaggcgccc acatcgtccg cggcggcgat gagcacggcg
     1201 ccggcgagcc cgccgagcac cacgatccgg cgggcggtgt gcgccacggt cgtcgctgcc
     1261 gccgcgccct gcccctccgc ggcggcgagc accggatagg cggcggtggc gagggggacg
     1321 gcgagcacgg catacggcag cagatagacg gcctggacgt attggtagcc gttgatcgtg
     1381 cctacggcgg cccggttatt ggccaaccag agcacagcca gcacggaaat ctgttgcgcg
     1441 agaagggcga tcaggcccgc tcccgcgaga cgccgggccg cggcggccac gcccgggggg
     1501 aagcgcagcg tggggtgcag ccggatcccc agccggcgcg ccgtcggcac cagcgtcgcc
     1561 accagcgcga gcacgcccag cgacgtgccg ccggcgagca cccagacggc ggcgctgggc
     1621 gatccgccgc gcagcacgag ccaataggcg acataggtcg cgatgacgac gaggctcgag
     1681 acgagcggcg ccaccgccgc ggcggcgaag cgttcgtgcg cgttcagcac cccggacagc
     1741 acgattccga tcccgtagag cacgatctgc ggcgcgaaga cccagagcat ccgggtgccg
     1801 agttcgaccg ccgcggcgga gtcgagcagc cagcgggaga tcagcggcgc gagtgcggcg
     1861 acgatcagca ccagaggcag cagcacggcg gcggcccagg tgagcaaggc cgaggcgatg
     1921 gcgtcggcct cggcgcgctc cccgcggccg agccgggcgg cgacgagcgg cacgacgatc
     1981 gccgcgagca cgccgcccgc ggcgatctcg aagacgacat tgggcaccgt gttggcgctc
     2041 tggtaggccg tgccgacggt cgtggtgccg acgcacgccg cgaagacgag catccgcacg
     2101 aacccggcga ggcgcgcggc ggccgtgagc acggcgatca ggcgcgcatt ggccccgacg
     2161 ctcaccgcgc ccgccccgcc ggtctcatgg gcgcccccag gcgtcgaggt cgcgcaggac
     2221 cggggtcgat tcgatcaccc gggtgaagct gacccgctcg gaggcgaggg tcagggccgt
     2281 cagcacggcc agggcgacgc cgcggccgag cggccccgag cgggcgacga gcgcggtgcc
     2341 gacgagggca ccggccgcgt tggcgccggt gtcgccgagc atcgtctcgc cgcgcaggtc
     2401 cgcggggagt gccccgaggg ccgcgccgac ggcgctcgcc gccgcgctct cccccagcag
     2461 ggccagcggt atgccgccca gcaccgtcgc cttgagggcg cggcccggcc gcaggtcgag
     2521 gaggttcgcc aggttggcgc tgcccgcgat gaccgcgccg ccgacgaggg tggcggggcc
     2581 gagccggcgg ccggcgtcac ggtcgacgag ggcggccccg accagtccgc tcgcgccgat
     2641 cccggcgatc ttcagcgccc ccgtcgtgac ccggccctgc cgcaacgccc cgaggtgccc
     2701 gcgcaggccc ttgctcgcac tgtcaccggc gagatcgtcg aggagtccgg ccgcgccggc
     2761 gccgacgcag gcgaccagtc cggcggccac gctccccata ccggtttgac gccggggcga
     2821 ggccagggca gccgcggcga gaccgagagc cgaccccgcg acatacccgg gaccctcgag
     2881 cagcgagatc gggcgaccgg cgtgattggt acgcgtccac cgcgctcccc cgcccggagg
     2941 ccgggtccgc agcagccgcg tcagcacggc ggtggccgcg cccgcgaccg cgcctgtcac
     3001 caacccgtcg ttcaccggtg gctacgtcag ttcgtggaag ggaacgggga cttggcgccg
     3061 gctgcgcggc cgtactgccc ggcgcggccg gcctcctgct cgaccagggc ggcgacgatg
     3121 ctcgcctggc cgatcgggtc gctggcgtcg tcgacgctgg agacctcgcg gcgcaccgcg
     3181 gcgcgggccc gggccgcggc gatgatcgag gtggagtccg ctgcggtcgg gtcgatggcc
     3241 gcggcgatga cgacgccgtc gctggcgtca tcgagggcag cggccagctc cgccagggcg
     3301 ggcagcgggt cggtggtgcc gctcgagccc tgggccgtgg cgacctgggt ggcgccgacg
     3361 acggtgacga ggttggcgcg ggttgccggg agggagcccg agacgtagcc gtcgctgacc
     3421 agccgggtcc aggccgcggc gcgcgcgaca gcgctcgggg cctccggtgc cgtcgtgccg
     3481 tcgggggttg ccggggccgc gagcagggcc ccgaggacgt cgcccagatc gggagcgtcc
     3541 tcggtgtcga cggtcaggcc cagggtcggc gcgagggtgc tggccagttg ctcgcgccgg
     3601 gtggtcgcct cctgcgagga atcgctccac gcctgctcga tccgcaccgg gccggagaca
     3661 ctggcgcctg cggcggagag ggtgtcctcg gtctcgacga cgagggagtc cggtgcaccg
     3721 gggagggtga cgacgacgac ggaccgctca gcgaggcgtc cgctgacgat tgccgggagt
     3781 tgcgcgttgg cgaagtcctc ctgttcctgc accgacttct cggacgcatc gagctggctc
     3841 cgcaaggcat ccttgtccgc gcgcagctgc gccacctgct ggtcgaggga gtcgctgatg
     3901 ccctccttga gcgggccggc gcccaggacg atgccgaccg cgagcgcgat gagcaccgag
     3961 gcgagcgaga cgaggtggta tcggaagtcg atcacgtgaa gatccccacg atcgcggccc
     4021 agaggtcgtc ccagcgggca ccgagcagtt gcaacagaat cgagccgccg ggtgtcgccc
     4081 acagtgccag cccgaggacg agcatgccga ccaggagcat caccagcatg ctgaccccgg
     4141 agatccggga gcgatagagg cggttgacgc ccttggcgtc gatgagcttg gacccgacgc
     4201 gcaggcgggt caggaacgtg ctcgccatgc cggcccggcc tttgtcgagg aattcgacca
     4261 gggtcgcgtg ggtgccgacg gccacgatga gagaggcgcc cttgtcgtcg gcgagcagca
     4321 tggcgacatc ctcgctggtg ccggttgcgg ggaagatcac cggctccacg ccgagcccgc
     4381 ggacccgctc ggcgcctggg gcccggccgt cgcggtaggc gtggacgacg atctcggctc
     4441 cgcaggtcag ggccttgtcg gacaccgagt ccatgtcgcc gacgatcagg tcgggtttgc
     4501 gcttggcctc cagcaacgcg tcggcgccgc cgtcgacccc gatgagcacc ggtttgaact
     4561 cgcgcatgta gtgctggaga gcctgcagat cctccttgta gtggtagccc cggaccacga
     4621 tcagggcgtg ccggccgtcg atcttggtcg cgatgtcggg cacgccgacg ccgtcgagca
     4681 gcagatcctt ctcgcggacc aggtagtcca tggtgttctg ggcgaaggcc tgcagctgac
     4741 cggacaatcc ctggcgcgcc agcgacaggg cgtcggcgac ggagtcgtcg tcctggacca
     4801 cccccgcgac aagggccggg gcctcgtccg cgaccgcatc ggcggcgaac acctggccgt
     4861 ggtcgaggcg cagccattgg ccttcgcgga tcgtcgtcat cgcgtcggga ccggcgtcgt
     4921 cgatgagcgg gatgcccgcg tcgagcagga tggtcggccc tacgttcggg tagcggccgc
     4981 tgatggagcg ggcggcgttg acgacggcgg cgggccggca cgccacgagg gcctcggcgc
     5041 tgacccggtc caggtcctcg tggtcgatga cggcgatctc gccgggctgc aaccgtttgg
     5101 tgaggttctt ggtgcgccgg tcgacgcgag ccatcccggt ggcgccggcg ggcaccggtt
     5161 ctcgggatcg gcgggacagg gaaacggcca tcgaggtcaa tcgtcgcacg cggtgcggcg
     5221 tgctcgcgcg tccgccaaaa gctccctcgc gtgtctgact gccgcagccg aatcggtgcc
     5281 ggccatcatc cgggcgatct cgcgttcgcg gtcgcgggcc tcgacgaccc gcacatcgct
     5341 ctgggagacc gagccgtcgt cggtcttggt gacgacgagg tgttgatcgg cgaaggccgc
     5401 gacctgcggc aagtgcgtca cgacgacgac ctgagcgtgc tcggcgagcc gggccagccg
     5461 ggcgccgatg tcgatcgccg cgcgaccgcc gacgcccgcg tcgacctcgt cgaagacata
     5521 ggtcggcacg ctcgtcgccg aggtgccgag aacgacctcc agcgccagca tcacccggga
     5581 gagctcaccc ccggaggcgg acttcgcgat cgggcgcggc ggcataccgg atccggagcg
     5641 gagcaggaac tcgacctcgt cgacgccgtc cttcccgtaa cggacctctc cgtcaccgcc
     5701 ggtgacggcg acctcgagcg tggccgagcc catcgccaga tgggccagtt cgtccgtgac
     5761 ggcgcccgcg agacgggtgc ccgcggcgtg ccgcaggtcg ctcacggtgc gggcggccgc
     5821 agcccgggtg atctcggcag ccgcgagctg ttcgcgcagc tcgggcaggt cgtcggcggc
     5881 cgtgtcgacg tcgagcaggc gggcgctggc ctgcgcgagc cacgtcaggg ccgactccag
     5941 gctgccaccg tgagccttgg ccagcccggc gagttcggcg cggcgctcgt gcacggcggc
     6001 caggcgggca tcgtccacgt cgatcccgtc ggcgtaggcg aacaggtcgg cgccgagttc
     6061 ggagacgagc accccgacct cgcgcagccg gcccgcgagt tcggggaggg cggggtcgtg
     6121 ttcgacgacg ccgtcgatga gcgcgccggc cgacgcgatg tgttcgctgg cgccggtcgc
     6181 gtcaggagcc gactcgtcgc ccaggaggta tgccgccgcg ctggccccgg cggcccgcaa
     6241 ctgttcgccg tgggcgaggc gttcggcctc ggcgcgcagg gcctcgtcct cgcccggttg
     6301 cgggtcgacg gcttcgatgc gctcgacccc ggcccgcagc aggtcgaggt cggccgtctg
     6361 ggtcgccacg agttcttcga ggcgggtgac ctgggcggcg agatcgcggc attgcgtgtg
     6421 gacgtcccga tagtgggtca gcgcggccgc cagttcgtcc ccgccgaact cgtcgaggag
     6481 ctgccggtgc tgctcccccg accgcagtcg ccactggtcg gcctggccgt ggacggcgac
     6541 gagattctcg gcgagttcac cgagggtgcc gaccggcacc gaacggccgc cggcgaaggc
     6601 gcgggatcgg ccgcccgccg cgacggtgcg cacgaggatc agcccgtcct cgacgtcggc
     6661 gccggcgtcg gcggcacgcg ccagcgcggg atggtcggca ggcaggtcga tcaggccctc
     6721 gacgacgagg ctgggcgcgg gcgcccggac gagagcggcg tcaccgcggg cgccgaagag
     6781 cagccccaga ccggtgacga ccatggtttt gcccgcgccg gtctcgccgg tgacgacgtt
     6841 gagacccgcg tgcaggggca ggtgcgccgc ctggatcacc cccagcgagc cgatccggat
     6901 ctcgctgatc accctaggga cccgcccggt tggggcgcgg accacgccag ccgtgcacgg
     6961 acaggtcgaa cttctccacc aatcggtcgg tgaacggttg ctccgacagg cgagccagtc
     7021 gcaccggctc ggccgagcgg catacctcca cccgggcacc ggccggcagg tcgacggcgc
     7081 gccggccgtc gcaccacagc accccttgcc cctcgttgcc gctgacgacc tcgaccgcca
     7141 gccgcgagcg cggcgaaagc accagcgggc gggcgaagag cgcatgcgcg ctgatcggga
     7201 cgacaagcat cgcgtccacg tcgggccaca ccaccggccc gcccgcggag aacgcgtatg
     7261 ccgtcgaccc cgtcggcgtg gccatcacca caccgtcaca accccaggtc gacagcggcc
     7321 ggccgtcgat ctcgaccgtg agctcgagca tccgctcgcg ggaggccttc tcgaccgtcg
     7381 cctcgttgag cgcccacgtg cgcgcgaact cgcgaccgtc gagccggctg accacatcga
     7441 gggtcatccg ctcggtgatc gcataatccc gatcgaggat ccgctcgatg gtcgcgtcgg
     7501 tgtcctcgcg ttcggactcg gcgaggaagc cgacgtgccc caggttgatg ccgagcagcg
     7561 gggcgtcgga accgcgggac agctcggccc cccgcaggat gctcccgtcg ccgccgagga
     7621 caagcaccag ctcacacccc tcggcgggcc ggtcgccgtc gccgatgctg accaggtcgt
     7681 ggccgggatc gaccgcctgc gcggtcatcg ggtccaagac gatgccgatg ccctcgccca
     7741 gcagccgctg ggcgacctcg cgggccaggt ggatggcgtc gggccggttg gggtgagcca
     7801 ccagcaggat gcggcggtgc gcgctgctca cgggtccctc ctgaccaggt cgtcgatctg
     7861 ctcctccaga gcctgccatg aggcactgac attgccgccc ttggccagcc acccaaggta
     7921 ctcccggttg ccgtgggtgc ccacgaccgg ggaggcgatc agcccacgca cgcacaggcc
     7981 ggcgtcgacc ccggcctgca gcacgtcgcg catggcgccg gcccggtcgg tggggtcgcg
     8041 gacgatgccc ttcttgtcga ggcggccgcg gccgacctcg aactgcggct tgatcagcag
     8101 tatgaggtcc gccgcctccc cgctcacccg ctccaggtcg gccatgacga gggtgaggga
     8161 gatgaaactg aggtccgcga cgagcaggtc gacggagccg ccgatgtcgt cgggcgtcag
     8221 gccgcggatc gagcggccgc tcagatcgtg tacccgcgga tccgagcgca ccggctcggc
     8281 gagctggtcg tgcccgacgt cgagggcgag cacctcgctg gcgccccgct cgaggaggat
     8341 ctgcgtgaag ccgccggtac tcgcccccac atcgagggcg cgcttgccgg cgaccgcgag
     8401 ccccgcgggc tcgaatgccg tgacggcggc gtcgagcttg tcggcgccgc ggcccacgcg
     8461 gatgttcagg tcgccgtcga tcgcgagccg gtcgcccggg ccgacgcgtg tggcgggttt
     8521 ggccgcgggg gcctcgttga gccggactcg cccggcctcc agcagttctc tggcctgccc
     8581 gcgggagcgg gccagaccgc gagccaccag cgcctggtcg agccgctcgc ccccgctcac
     8641 ccgtccaggt cgccgagccg ggcgcggagg acgccctcga gcgcgctgcc ctgctcgagt
     8701 accgcgtcca ggtccgctcc gctggtctcg gcgaggtcgc gcagggcgct gtcgatcacc
     8761 aggtcgccgg tatgcggtgg gctcgcctcc tctgtcctgt ccggctcggt gttggcgggc
     8821 tcagtgctgg ccggctcggt gttggcgggc tcagtgctgg cgggctcagt gctggcgggc
     8881 tccgtctcga tcccctggga gtggaacgcc tcgtccgacg cgtccggcag ctgcgtgggc
     8941 tcactcacga ggtgctcgcc gacccgccgg tccgtcgtcg ggtcgcggcc gcggtgggtg
     9001 ccgccttccg cggcgaggcg gccttcttcg tggtgccctt cgcggcccgc tggcgggtat
     9061 tcgactgcgg cgcagccttt ttcgcgcccg tcgcccgagc ggccgacttc gtggccgttg
     9121 tcttcgc
//
LOCUS       DAOPJG010000139         9087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8061_length_9087_cov_7.708370, whole genome shotgun sequence.
ACCESSION   DAOPJG010000139 DAOPJG010000000
VERSION     DAOPJG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8061_length_9087_cov_7.708370"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /gene="purF"
                     /locus_tag="PKU75_12000"
     CDS             <1..943
                     /gene="purF"
                     /locus_tag="PKU75_12000"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPF81648.1"
                     /translation="PRLRGAFSFVFMDEHTLYAARDPQGIRPLVLGRVERGWVVASET
                     AALDIVGASVIREIEPGELIAIDEDGLRSRIWAAPEPKGCVFEYVYLARPDTTIRGRV
                     VHESRVEMGRTLAREHPVDADLVIPTPESGTPAAVGYAQQSGIPYGQGLVKNAYVGRT
                     FIQPNQTIRQLGIRLKLNPLREVIRGQRLVVVDDSIVRGNTQRALVRMLREAGAAEVH
                     VRISSPPVRWPCFYGIDFATRAELIATGLEVEEVCRSIGADSLGYISEAGMIAATSQA
                     PAQLCTACFTGSYPVQLPDEERLGKGLLEYQLPLDVS"
     gene            996..1382
                     /locus_tag="PKU75_12005"
     CDS             996..1382
                     /locus_tag="PKU75_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HPF81649.1"
                     /translation="MNLEKVVFAFFVVLAATLNFGFWYGDLTDARVHNVYELYAAVIV
                     NIIALVFKFGDRTQIGALHLATSLVAVIQLIAAATVWIWAREATEAGLTVEHMSSVVS
                     LSAGALLANIVSIILLVIETASYQRR"
     gene            1389..3119
                     /locus_tag="PKU75_12010"
     CDS             1389..3119
                     /locus_tag="PKU75_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPF81650.1"
                     /translation="MGNPLLFVRPLLRMRAVLRRLPVDIPTDIPATDAIFLVLRRMRW
                     PLISLIIVFTIAVFGLSAAPGFDADGNPRKLTVFESFYVMSYTATTIGFGEIPQTFTT
                     QQRLWVTFTIYATVIVWAVAIGTMMALVQDEVFRDAVAAQRFGRKVRGIRENFHILAG
                     YGQAGRQVGAGLDWLGRRFVVVERLGARVDALGTDQLAVDTPGVEGDAANPALLGMAG
                     LGSDHCKGVLALTDDDDTNLGVVQAVHLLRPEVPVIARCTDRRIGAYMESFGAEAVIN
                     AYDRFGGYLTLALQKPAVYQLITWLMSPGGTPLPDRGTAVPGGKWLVCAEGQFRDEID
                     RDLLAAGYTVDHVEPDGALDTLDSIVGFVGGTDRDTTNLALAATVRTDAPDVFVCLRQ
                     TKASSAALYEAFAPDSLFVPTELVAREAFARIVTPRTWGFLEYAMTQDDEWAEDLVRR
                     LVDRCGSRSPDPYGLTIDTTSAPALARWLRRNTFTIRDLLSNPDDRDDATGNAILALG
                     RDGQIAYGPDLDTALEFGDELLVVSQRRSFSRLNDVLFNDAAVEYVATGREVPSTWIW
                     RKFTGSRRGA"
     gene            3183..4313
                     /gene="purM"
                     /locus_tag="PKU75_12015"
     CDS             3183..4313
                     /gene="purM"
                     /locus_tag="PKU75_12015"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPF81651.1"
                     /translation="MSEGPITYAAAGVDVEAGDKAVELMKASVARATRPEVLGGLGGF
                     AGMFDASALARMARPVLATSTDGVGTKVAIAQALDIHDTIGFDLVGMVVDDIVVCGAE
                     PLFMTDYIACGKVVPERIAAIVSGIAGACAQARTALVGGETAEHPGLLETDEYDVAGA
                     ATGVVEHAEVLGAHRVLPGDVVLALASSGLHSNGYSLVRRVIAAAGWGLERHVAEFGR
                     TLGEELLTSTRVYAADLLDVIRDERVRDGGGIHALSHVTGGGLAANLARVLPGGVHAT
                     LDRSTWTPPPVFSVIEGLGRVPQPDLERTLNMGVGFVALLPEAAADAAVALLGERGIH
                     AWPIGHVDTEAAAAVEPGIEVVRGAKGVTGGSVQVLGEYARS"
     gene            complement(4418..6139)
                     /locus_tag="PKU75_12020"
     CDS             complement(4418..6139)
                     /locus_tag="PKU75_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81652.1"
                     /translation="MTRSRERDERDLARRERATPSPLADTISNVLDPGWVVLALIGTI
                     AAASTPSWGAAAGWAALAVAFTVVVPYAALAVLVRSGRVDDRHLVRRDQRLVPALVAL
                     AGAAAGVALLWRLGAPRDVLAVMLAMAGGVLVLGLATLVAKVSLHTAVIAGAVAVLVL
                     RFGWWALALAPVAALVGWARWRAGRHTVVQIVTGGLLGALVAGGVFALAREPVRGTLA
                     EPAPTHLRAVEVDLPGPVSALAGGGEGLWIGTYDAAVRPAAGLTWWDGASGTGQRVPV
                     HALSSYAFGGQWRALAVRESALLGIAGQVGGAHGNVRWTIWRGDRSGVTEEEQEFWTF
                     GGYDMGGLSGAGYVGADPLVVGGWKSRSTGLDIATWHASGATWARDDVPAALASSPEV
                     LRAATAVIPRGPESVLLGSTTHLGAGFVTVAPTVWISAGGGARPVWTAQELPLPGGVS
                     GQGRAEAGHCPGHGPCLLAGRVAGRLVAWRGDLPAAVPLQLPGVALADTDRVLGTGVT
                     EHDLWIAVQRAAGPVLLRARDSRAAADLRWSVEAPPAARLTAAAAFDGRFCVATGPPA
                     RLWCTPT"
     gene            complement(6144..6686)
                     /locus_tag="PKU75_12025"
     CDS             complement(6144..6686)
                     /locus_tag="PKU75_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPF81653.1"
                     /translation="MSTVEPLADDWLVLIDPQAIFADPATSQWGSPMFAGALPEMIRL
                     ATAYGPQRTIVTRFVADPGLGGSWATYYEDWAFALVPDADPLYAVDPALAPLAGHVVT
                     EATFGKWGDQLRGLVGPQPRLTLGGVSTDCCIIATALPAADAGATIRVPAAACAGSSL
                     ENHAAALQVMALFGPQIVIC"
     gene            complement(6683..8242)
                     /locus_tag="PKU75_12030"
     CDS             complement(6683..8242)
                     /locus_tag="PKU75_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HPF81654.1"
                     /translation="MTASSGAERVRSLGVETTGIEIIGESERTASPKDLFWPWFAANV
                     SVFGISYAAFVLGFGISFWQAALVTVLGIVISFLLCGIIAIAGKRGSAPTMILSRAPF
                     GVTGQKLPGVISWLVSIGWETFLSIMAVLATATVFERLGWSSGTTVKIIAAAVVAAMI
                     VLASVAGYHIIMKMQSYLTWITGIVTILFVIIAAKEIDWGTVSAIPSGPWQAVVGALV
                     MLMTGFGLGWINIAADWSRYQHRNASGSAIVFWNTFGGAIAPVLLVLFGLLLAGSNEE
                     VRNGIGADPIGSLAQVLPTWFLVPFLIAVILSLVSGAVLGIYSSGLTLLSLGVKIPRP
                     AAALIDGVILTLGTIWVVFFAKNFLGPFQSFLITLGVPLAAWAGIMIADIALRRRDYD
                     DNALFDSRGVYGSVDWISIGTMIVMSILGWGLVVNNFAADASWNNWQGFLLEPLGLGT
                     FVDDPAGAYWEGNWPYANLGVLLALAGSFLITYLLRRPKVRDQEMARGYAGYGPGAAA
                     DGGATAYGPRA"
     gene            complement(8358..8555)
                     /locus_tag="PKU75_12035"
     CDS             complement(8358..8555)
                     /locus_tag="PKU75_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HPF81655.1"
                     /translation="MGRGRAKAKQTKVARDLKYYSPKTDLSALERELHGAAPTAEPLV
                     SDEPDGDDEYEDDYARWASGR"
     gene            complement(8718..>9087)
                     /locus_tag="PKU75_12040"
     CDS             complement(8718..>9087)
                     /locus_tag="PKU75_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81656.1"
                     /translation="VVAVVLVVAVVLVVAVVLVVDGVLVVDGVAEDVAVAPGGVVEPV
                     QVEVAGAVVLDITPGVAVAAPCCEVPAPHPARPTAAAVRTSAQVRRFMMIPPEVGFGG
                     ACALLTSSDVPRRPVVGVNR"
BASE COUNT         1397 a         3353 c         3101 g         1236 t
ORIGIN      
        1 cccgcggctg cggggcgcgt tcagtttcgt cttcatggac gaacacacgc tgtatgccgc
       61 ccgcgacccc caaggcatcc gcccgctcgt cctcggccgg gtcgagcgcg ggtgggtggt
      121 cgcctccgag accgccgccc tcgacatcgt cggcgcctcg gtgatccgcg agatcgagcc
      181 cggcgaactg atcgcgatcg acgaggacgg cctgcgctca cgcatctggg ccgcgcccga
      241 acccaagggc tgcgtcttcg aatacgtcta tctcgcccgc cccgacacga cgatccgggg
      301 ccgggtcgtc cacgagtccc gggtcgagat gggccgaacc ctcgcccgag agcaccccgt
      361 cgacgccgac ctcgtcatcc ccaccccgga gtcgggcacc ccggccgccg tcggctacgc
      421 ccagcagtcc gggatcccct acggtcaggg cctggtgaag aacgcctacg tcggccggac
      481 cttcatccag cccaaccaga ccatccgcca gctcggcatc cgcctcaaac tcaacccgct
      541 gcgcgaggtc atccgcggcc agcggctggt ggtcgtcgac gactcgatcg tgcgcggcaa
      601 cacccagcgc gccctggtgc ggatgctgcg cgaagccggc gccgccgagg tgcacgtgcg
      661 catctcctca ccccccgtgc gctggccctg cttctacggc atcgacttcg ccacccgggc
      721 cgaactgatc gccaccggct tggaggtgga ggaggtatgc cgctccatcg gcgccgactc
      781 cctcggctac atcagcgagg ccggcatgat cgccgccacc tcgcaggccc cggcccagct
      841 ctgcacggcc tgtttcaccg gcagctatcc cgtgcagcta cccgacgagg agcgcctcgg
      901 caagggcctg ctggagtatc aactcccgct cgacgtgagc tgaccggcga acgcgccgca
      961 tacccagacg ctgcgcgctg ggtatcgtca gcctcgtgaa cctcgaaaag gtggtcttcg
     1021 ccttcttcgt cgtcctcgcg gcgacattga atttcggctt ctggtacggc gacttgaccg
     1081 acgcgcgcgt gcacaacgtc tacgagctgt atgccgcggt gatcgtcaac atcatcgccc
     1141 tggtgttcaa gttcggggat cgcacccaga tcggcgccct ccacctggcc accagcctgg
     1201 tcgccgtcat ccagctgatc gccgcagcca cggtgtggat ctgggcgcgc gaggcgaccg
     1261 aagccgggct caccgtcgag cacatgagca gcgtcgtctc cctctccgcc ggcgccctcc
     1321 tggccaatat cgtctcgatc atcctgctcg tcatcgagac cgcgagctac cagcggcgct
     1381 gaccggcctt ggggaacccg ctgctcttcg tccgcccgct gctgcggatg cgggcggtgc
     1441 tgcgtcgcct gccggtcgac atacccaccg acatcccggc gacggatgcc atcttcctcg
     1501 tcctgcgccg catgcgctgg cccctgatca gcctcatcat cgtcttcacg atcgcggtct
     1561 tcggcctgtc cgccgccccg ggcttcgacg ccgacgggaa tccgcgcaag ctcaccgtct
     1621 tcgagtcctt ctacgtcatg agctacaccg ccacgacgat cggattcggc gagatcccgc
     1681 agacgttcac cacccagcaa cggctctggg tcaccttcac catctacgcc accgtcatcg
     1741 tctgggccgt cgcgatcgga acgatgatgg ccttggtgca ggacgaggtc ttccgtgatg
     1801 ccgtcgcggc gcaacgcttc ggccgcaagg tgcggggtat ccgcgagaac ttccacatcc
     1861 tcgcgggcta cgggcaggcc gggcgccagg tcggcgcggg gctcgactgg ctcgggcgcc
     1921 gcttcgtcgt cgtcgagcgt ctcggtgcgc gcgtcgatgc gctcggcacc gaccaactgg
     1981 ccgtcgacac cccgggcgtc gagggcgatg ccgccaatcc ggcgctgctc gggatggccg
     2041 gcctcggcag cgatcactgc aagggtgtct tggccctgac cgacgacgac gacaccaacc
     2101 tcggggtcgt ccaggccgtc cacctgctgc gtccggaggt cccggtcatc gcccggtgca
     2161 ccgaccggcg catcggtgcc tatatggaga gcttcggcgc cgaggccgtc atcaacgcct
     2221 acgaccgatt cggcggctac ctcaccctcg cgctgcagaa gcccgcggtc taccagctga
     2281 tcacgtggct gatgagcccc ggcggcacac cgctgccgga ccgcggcacc gccgtccccg
     2341 gcggcaagtg gctggtgtgc gccgaaggcc agttccgcga cgagatcgac cgcgacctgc
     2401 tcgccgccgg ctacaccgtg gaccacgtcg agcccgacgg cgccctcgac accctcgact
     2461 ccatcgtcgg attcgtcggc gggaccgatc gggatacgac caatctggcg ttggcggcga
     2521 cggtccgcac ggacgccccc gacgtcttcg tctgcctgcg ccagaccaag gccagctccg
     2581 cggcgctgta tgaggcgttc gccccggaca gcctcttcgt ccccaccgaa ctcgtggccc
     2641 gcgaggcctt tgcccggatc gtgacgccgc gcacgtgggg attcctcgag tacgccatga
     2701 cgcaggacga cgagtgggcc gaagacctcg tccgccggct cgtcgatcgc tgcggctcgc
     2761 gatcccccga cccctacggc ctgacgatcg acaccacctc cgctccggcg ctggcccgct
     2821 ggctccggcg gaacacgttc acgatccgcg acctgctgag caaccccgac gaccgcgacg
     2881 acgccaccgg caacgcgatc ctcgcgctcg ggcgggacgg gcagatcgcc tacggccccg
     2941 acctggatac agcactggag ttcggcgacg aactgctcgt cgtgtcccag cggcgctcct
     3001 tctcccggct caatgacgtg ctcttcaacg acgccgcggt ggagtatgtc gccaccggcc
     3061 gcgaggtgcc gagcacctgg atatggcgca agttcaccgg cagccgccgc ggcgcctgac
     3121 gccacccacc gggcgcggac gcggcatacg acccgctgcc ctcggcacgt aggctggccc
     3181 gcgtgagcga aggcccgatc acctatgccg cagccggagt cgacgtcgag gcgggagaca
     3241 aagccgtcga gctgatgaag gcgtccgtcg cgcgggccac ccgtccggag gtgctcggcg
     3301 ggctcggcgg tttcgccggg atgttcgacg cctcggcgct ggcccggatg gcccgcccgg
     3361 tcctcgccac ctctaccgac ggcgtgggca ccaaggtcgc catcgcgcaa gccctcgaca
     3421 tccacgacac catcgggttc gatctggtcg ggatggtcgt cgacgacatc gtcgtctgcg
     3481 gagccgaacc gctgttcatg accgactaca tcgcgtgcgg caaggtggtc cccgaacgga
     3541 tcgccgccat cgtctccggc atcgccggcg cctgcgccca ggcccgcacc gccctcgtcg
     3601 gcggggagac ggccgagcac cccgggttgc tcgagacgga cgagtatgac gtggccggcg
     3661 ccgccacggg tgtcgtcgag catgccgagg ttctgggtgc gcaccgggtg ctccccggcg
     3721 acgtcgtgct cgccctcgcg tcgtcgggcc tgcactccaa cggctattcg ctggtgcgcc
     3781 gcgtcatcgc ggcggccggg tgggggctcg agcggcacgt ggcggagttc gggcgcacgc
     3841 tgggcgagga gctgctcacc tccacccgcg tgtatgccgc cgatctcctc gacgtgatcc
     3901 gcgacgagcg cgtacgcgac gggggcggca tccacgccct ctcgcacgtc accggcggcg
     3961 gccttgccgc caacctcgcc cgcgtgctgc ccgggggcgt gcacgccaca ctcgaccggt
     4021 ccacctggac cccgccgccg gtcttctcgg tcatcgaagg cctgggccgg gtgccccagc
     4081 ccgatctcga acgcaccctc aacatgggcg tcggcttcgt cgccctgctg cccgaggccg
     4141 cggccgacgc cgccgtggcg ctgctcggcg agcgggggat ccacgcgtgg ccgatcgggc
     4201 acgtcgacac cgaagcggcc gccgcggtcg aaccgggcat cgaggtggtc cggggcgcca
     4261 agggcgtcac cggcggctcc gtccaggtgc tcggggagta cgcccgctcc tgaaacccgc
     4321 gctcctcaaa cgcggccgcc cttctcgtgc cgcgccgggt cgtgccggcc ccgagccgag
     4381 gccgaccggc ggtatgccgc gccgtcaggc gggagtgtca ggtgggagtg caccacagcc
     4441 gggccggggg tccggtggcg acgcagaagc ggccgtcgaa ggccgcggcg gcggtcagcc
     4501 gggcggcggg cggggcttcg acgctccacc gcagatcggc tgcggcgcgg gagtcgcggg
     4561 ctcgcaggag gaccgggccc gcggcccgtt gcacggcgat ccagaggtcg tgctcggtga
     4621 cgcccgttcc caggacgcga tcggtgtcgg ccagcgccac gcctggtagc tgcagcggca
     4681 cggccgcggg caggtcgcca cgccaggcga ccaggcgccc cgcgacgcgg ccggccagca
     4741 ggcacggccc gtgacccggg cagtggccgg cctcggcccg gccctgcccg ctgacccctc
     4801 ccggcagcgg caactcctga gccgtccaca ccggccgcgc cccgccgccg gcggagatcc
     4861 acacggtcgg cgcgaccgtc acgaagccgg cgccgaggtg ggtcgtggac cccaacagca
     4921 cactctcagg gccgcgggga atgacagccg tggccgcccg cagcacctcc ggagaactcg
     4981 ccagcgccgc gggcacgtcg tcgcgtgccc acgtggcccc ggatgcgtgc caggtggcga
     5041 tgtccagccc ggtcgagcgg gacttccagc cgccgacgac gagggggtcg gcgccgacat
     5101 accccgcgcc gctcagcccg cccatgtcat agccgccgaa ggtccagaac tcctgctcct
     5161 cctcggtcac gcccgaacgg tcgccgcgcc agatcgtcca gcgcacattg ccgtgcgcac
     5221 cgcccacctg tcccgcgatc cccagcagcg cggactcgcg taccgcgagc gcgcgccact
     5281 gcccgccgaa ggcataactc gacagggcgt ggaccggcac ccgctgcccc gtcccggagg
     5341 cgccgtccca ccacgtcagc ccggcggcgg gacggacggc agcgtcatat gtcccgatcc
     5401 acaggccctc cccgccgccc gccagcgcgc tcaccggccc cggcaggtcg acctcgacag
     5461 cgcgcaaatg ggtcggcgcc ggctcggcca gcgtgccgcg caccggctcg cgggccaggg
     5521 cgaacacgcc gcccgccacc agcgcgccga gcagcccgcc ggtgacgatc tggacgacgg
     5581 tatgccgccc ggcccgccac cgcgcccacc ccaccagggc ggcgaccgga gccagcgcca
     5641 aggcccacca cccgaaacgg aggacgagga ccgccaccgc tcccgcgatg acagccgtat
     5701 gcagtgacac cttggccacg agggtggcca gcccgaggac cagcaccccg cctgccatcg
     5761 cgagcatcac cgcgagcaca tcgcgcggcg cgccgagccg ccacaacagc gccacccccg
     5821 cggccgcgcc ggccagggcg acgagcgcgg gcacgagccg ctggtcccgg cgcaccaggt
     5881 gccggtcgtc gacccggccg cttcgcacca gcacggcgag ggcggcatac ggaaccacga
     5941 cggtgaacgc gacggcgagt gcggcccagc cggcggccgc accccaggac ggtgtcgatg
     6001 cggcggcgat cgtgccgatc agggccagga cgacccagcc ggggtcgagg acgttcgaga
     6061 tcgtgtcggc gaggggggag ggggtcgcgc gctcgcggcg tgcgaggtcg cgctcgtcgc
     6121 gttcgcggga cctcgtcacc gggtcagcag atgacgatct gcgggccgaa cagggccatg
     6181 acctggagag ctgctgcgtg gttctccagg ctcgacccgg cgcaggcggc agccggcacc
     6241 cggatcgtcg cccccgcgtc agcggccggc agggcggtgg cgatgatgca gcagtcggtg
     6301 gagaccccac ccagcgtcag ccgcggctgc ggccccacga ggccccgcaa ctgatcgccc
     6361 cacttgccga aggtcgcctc ggtgaccacg tgcccggcga gcggggcgag ggcgggatcg
     6421 acggcataca gcgggtcggc gtcggggacg agggcgaacg cccagtcttc gtagtacgtc
     6481 gcccacgatc cgccgagccc cgggtcggcg acgaagcggg tgacgatggt gcgctgcggg
     6541 ccgtatgccg tggcgagccg gatcatctcc ggcagcgccc cggcgaacat cggcgaaccc
     6601 cactggctgg tcgccgggtc ggcgaagatc gcctgcgggt cgatcaggac cagccagtcg
     6661 tccgcgagcg gttcgacggt gctcacgcgc gcgggccgta tgccgtcgcc ccgccgtcgg
     6721 ccgcggctcc ggggccgtag ccggcgtagc cgcgggccat ctcctggtcc cggaccttcg
     6781 ggcggcgcag gaggtaggtg atcaggaagg agccggccag ggcaagcagc accccgaggt
     6841 tggcgtaggg ccagttgcct tcccagtagg cgcctgcggg gtcgtcgacg aaggtgccga
     6901 ggccgagcgg ttcgaggagg aagccctgcc agttgttcca gctcgcgtcg gcagcgaagt
     6961 tgttgacgac caggccccac ccgaggatcg acatgacgat catcgtgccg atggagatcc
     7021 agtcgacgga cccgtagacg ccgcgggagt cgaagagggc gttgtcgtcg tagtcgcgcc
     7081 tgcgtagcgc gatgtcggcg atcatgatgc ccgcccaggc cgccaacggg acgccgagcg
     7141 tgatgaggaa cgactggaac ggcccgagga agttcttggc aaagaagacc acccagatgg
     7201 tgccgagggt gaggatgacg ccgtcgatca gcgccgccgc gggccggggg atcttcaccc
     7261 cgaggctgag cagggtcagg ccggaggagt agatgccgag caccgcgcca ctgaccaggg
     7321 acaggatgac ggcgatgagg aagggcacga ggaaccacgt cggcagcacc tgggcgagcg
     7381 atccgatcgg gtcggcgccg atgccgttgc ggacctcctc gttggagccg gccagcagga
     7441 gcccgaacag cacgagcagc accggcgcga tggcgccgcc gaaggtgttc cagaagacga
     7501 tggcgctgcc ggaggcgttg cgatgctgat agcgcgacca gtcggcggcg atattgatcc
     7561 agcccaggcc gaagccggtc atcagcatca cgagggcccc gacgacggcc tgccacggcc
     7621 cgctcgggat ggccgagacg gtgccccagt cgatctcctt ggcggcgatg atgacgaaca
     7681 ggatcgtgac gatgccggtg atccaggtca gataggactg catcttcatg atgatgtggt
     7741 agccggccac cgaggcgagg acgatcatcg cggccacgac ggcggcggcg atgatcttca
     7801 cggtcgtgcc ggaactccag ccgagccgtt cgaacacggt cgcggtcgcg aggacggcca
     7861 tgatcgacaa gaaggtctcc cagccgatcg agaccagcca ggagatgacg cccggcagct
     7921 tctgcccggt gacgccgaag ggcgcccgcg acaggatcat cgtcggggcc gacccccgct
     7981 tgccggcgat ggcgatgatc ccgcacagca ggaaggagat gacgatcccg aggaccgtca
     8041 ccagagccgc ctgccagaag gagatgccga agccgagcac gaatgcggca tacgagatgc
     8101 cgaacacgga cacattggcc gcgaaccacg gccagaagag gtccttgggg gaggcggtcc
     8161 gctcggactc gccgatgatc tcgatgcccg tggtctcgac cccgagcgag cgcacgcgtt
     8221 ccgcgcccga actagcagtc atacgccgaa gcgtgccaga tcggcccccc tgcgtggggg
     8281 atgctgcacg acatcgacca ccgtgcccga acgccgggcc tggtggaggg ggtccctgtc
     8341 agctcaggtg agtcaggtca acgaccggaa gcccagcggg cgtagtcgtc ttcgtactcg
     8401 tcgtcaccgt cgggctcgtc gctgacgagc ggctcggcgg ttggtgctgc tccgtggagc
     8461 tctcgctcaa gcgcgctgag atcggtcttc ggggagtagt acttcaaatc ccgagcaacc
     8521 ttggtctgct tggctttggc ccggccgcgc cccatggcgt gaccccctca tgcgtctagc
     8581 gggacggcat cactccggcc ggtcggtgat cggccttcgc gcagcggtcc cggtgatgtg
     8641 tgttctcgtg gtgctaacgc tacatggtgt ggcgaagatt ccgcgacgga caaagccgtc
     8701 gggacgtgac ggcttcgtta tctgttcacc ccaaccacag gtcggcgtgg gacgtcggaa
     8761 gaagtgagaa gggcgcacgc cccaccgaaa ccgacctcgg gaggaatcat catgaaccgc
     8821 cggacctggg cacttgtcct gaccgctgcc gcggtcggcc tggccggatg cggcgccggc
     8881 acctcgcagc agggcgccgc gaccgccacc cccggcgtga tgtcgagcac gaccgcgccc
     8941 gccacctcaa cctggactgg ctcgaccact cctcccggcg ccaccgcgac gtcctcggcc
     9001 acgccgtcca cgaccagcac gccgtccacg accagcacga cggccacgac cagcacgacg
     9061 gccacgacca gcacgacggc cacgacc
//
LOCUS       DAOPJG010000140         9087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8062_length_9087_cov_6.338020, whole genome shotgun sequence.
ACCESSION   DAOPJG010000140 DAOPJG010000000
VERSION     DAOPJG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8062_length_9087_cov_6.338020"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PKU75_12045"
     CDS             <1..539
                     /locus_tag="PKU75_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPF81657.1"
                     /translation="AGQVADPQRALERIAGLGRTALTELDRLVVHLRDPRQEVAVSAP
                     PRLLDIDELLAVPLRESGVDVKVRIGDGLDLADADVLAVYRMTQEALTNIARHAGARC
                     AWVDLQRTGDRVRLRVSDDGVGPATRAAEPARGLGLVGIAERAAAYGGTAELLSRPGG
                     GSTLDVVLVVPTVSRRAR"
     gene            536..1180
                     /locus_tag="PKU75_12050"
     CDS             536..1180
                     /locus_tag="PKU75_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF81658.1"
                     /translation="MIRVAVVDDQDLVRQGLALILRAQPDLDVAVEAADGQECLDAVR
                     ADPRIDVALVDIRMPGLDGLAATRALAAVPHAPAVIVVTTFDEDSYVLDAIAAGARGF
                     LLKRCSGRELVEAVRRVAAGDSILSPDVTGAVLDRVRALGPATLADLTPFALTERERE
                     ILALLGAGLTNAEMTVRLHLSMSSVKTHVSNVLAKTGSRDRVQAAILAIRAGLS"
     gene            1307..2242
                     /locus_tag="PKU75_12055"
     CDS             1307..2242
                     /locus_tag="PKU75_12055"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HPF81659.1"
                     /translation="MHTPAAVPTQPSWRRTLAAYVALTKPRIIELLLVTTVPTMFLAQ
                     RGVPSLWLVVATLIGGSLSAGSANTYNMVYDRDIDAHMGRTANRPLVTGEVTPRAALI
                     FATALALISTIWFAVFVNGLSAALSLAAIVLYAVGYTMILKRRTAQNIVWGGIAGCMP
                     VLIGWAAVTGTVSWAAVVLFLVIFFWTPPHYWPLSMRFKEDYAQAGVPMLPVVEGNVG
                     VGRQIVAYSWVMVVTSLVLIPVAPMGWLYAVAAVLSGGLFLAEAHRLLKAAKADASLA
                     QLKPMRLFHYSITYVTVLFLAVAIDPLVFHALPWG"
     gene            2446..2634
                     /locus_tag="PKU75_12060"
     CDS             2446..2634
                     /locus_tag="PKU75_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81660.1"
                     /translation="MNIHTGPDFVASELHYRLESATPTTTSPARSHRRRGLRALLRRL
                     TTPEYRPWPDGDGPTQVP"
     gene            2845..3234
                     /locus_tag="PKU75_12065"
     CDS             2845..3234
                     /locus_tag="PKU75_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF81661.1"
                     /translation="MKIRAIMIDCTDHYAQCRWWESVLDDYADAPDDPNYPDDETMTL
                     VGPEAQPWLCFQRVEDSKRTKNRLHFDLHPLGRTRDEEVERLLELGATVVGDHRRPNG
                     SGWVTMADPEGNEFCVERSEAEMAAGQ"
     gene            complement(3278..3556)
                     /locus_tag="PKU75_12070"
     CDS             complement(3278..3556)
                     /locus_tag="PKU75_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPF81662.1"
                     /translation="MKASRTLAESCHEAGVRVPDDIAIAGFDDIPEGRYHTPTLTTIA
                     PDLGALTMSILDLLIRRIEGGAGPAIRVEVPWSLQVRESTAGQTLGPA"
     gene            3486..3671
                     /locus_tag="PKU75_12075"
     CDS             3486..3671
                     /locus_tag="PKU75_12075"
                     /inference="COORDINATES: protein motif:HMM:NF018998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 family protein"
                     /protein_id="HPF81663.1"
                     /translation="MAISSGTRTPASWQDSAKVREAFMSGDSPADFPEEHYEADWTGR
                     FTLEQLNATGRRGMGLD"
     gene            complement(3681..>4182)
                     /locus_tag="PKU75_12080"
     CDS             complement(3681..>4182)
                     /locus_tag="PKU75_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPF81664.1"
                     /translation="TCSVPFADLRLVRVSYWGFDQTPHTGELVLNEDAVDAVVSALGA
                     LYEARFPIRKMVTVEDYGADDEMSMRDDNSSAFNGRFVEGTTSCSQHAYGRAIDINPL
                     ENPMVKDGKVYPATAGDFVDRDQEATGMIHAGDVVVRAFAGVGWSWGGDWHSLKDWQH
                     FSANGR"
     assembly_gap    4183..4282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4283..5199)
                     /locus_tag="PKU75_12085"
     CDS             complement(<4283..5199)
                     /locus_tag="PKU75_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HPF81665.1"
                     /translation="MTTLDHDRPRLTAEHRGTTTPWLRRILLLNVIVEVLIVVTGGLV
                     RLTGSGLGCPSWPQCVPGSYTPVREQAEGFHKYIEFGNRTLTGLVGFVAIAAVLAIWK
                     WTDRRRALLVPALLVLVGVGVQAVLGGITVRTGLNPATVAAHFLVSMGLVAAAMAAFV
                     ESGMPPGPRRHTAPQIVRQLGWATVALAVGVLVLGTVVTGSGPHSGDADEPARLSFDP
                     RSASWLHADSVMLFVGLVVAMVVATRISSHSSHLRDAWRAVLLVTLAQGAVGYTQYFT
                     KLPEALVLTHMLLASLLVVALVAAIKQFKR"
     gene            5280..6950
                     /gene="pgi"
                     /locus_tag="PKU75_12090"
     CDS             5280..6950
                     /gene="pgi"
                     /locus_tag="PKU75_12090"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPF81666.1"
                     /translation="MTIVDPTTTTAWATLTGLKTAYTPDLRAAFAGDPERAARFTRTC
                     ADLYVDLSKNLVDDHILEALVALADEVGLGDRRDAMLSGERINVTENRSVLHTALRRP
                     AGDSLVVDGTDVMPEVHEVLDKIYAFADKVRDGEWVGVTGKPIETVVNIGIGGSDLGP
                     VMVYEALKPYVHERIQCRFISNIDPTDSGEKLSDLDPETTLFIVASKTFTTLETLTNA
                     RMARDWLWRRLIEQGAIEDTEEARKGAVAKHFVAVSTALDKVADFGIDPANAFGFWDW
                     VGGRYSVDSAVGTVVCVAIGKENFADFLGGFHAVDEHFATAPWAQNVPALMGLLNVWY
                     VNFFKAASHAVLPYAQYLHRFAAYLQQLTMESNGKGVRADGEPVTTETGEVFWGEPGT
                     NGQHAFYQLIHQGTQLIPCDFIAMANTAYPTKDGDADVHELFLANFFAQTKALAFGKT
                     AEEVRAEGVAEELVSAKVFEGNRPTTSIMAPALTPSVVGQLIALYEHITFTQGVVWGI
                     DSFDQWGVELGKQLAMQIAPAISKDDAALEQQDGSTRGLIAYYRSQRS"
     gene            complement(7118..8491)
                     /locus_tag="PKU75_12095"
     CDS             complement(7118..8491)
                     /locus_tag="PKU75_12095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPF81667.1"
                     /translation="MSVSTDTTFGTLGVPAKLTAALAQRGITTPTPIQAATLPDSLAG
                     RDVLGRGRTGSGKTYAFLLPLVARLASAPSRRMPKRPRALILAPTRELVAQIEESLAP
                     LAAATGLNSRTVFGGVGQNPQVAALRKGVDIVIACPGRLEDLLGQGHLSLDAVEVTVL
                     DEADHMADLGFLPVVRRLLDRTPREGQRMLFSATLDAGVDVLAKRYLSRPVTHEADSA
                     QSPVAEMDHHVLHVGPAHHLPVLLDLTAAPGRTIVFTRTKHRAKKLARQLNAAGVPAV
                     ELHGNLSQNARTRTMDAFHSGAAGTLVATDIAARGIHVDDVALVIHADPPVEHKAYLH
                     RSGRTARAGNSGTVVTMMLDDQVRDVRDLTRKAGIRPTITRVNPAHPLLRQLAPGTRD
                     FPGGYAATPPAAPSPGRARQPRSGQPSRSAQSPGPGRADRARRESQPAANPGNRARRR
                     PQRQTAR"
     gene            complement(8685..>9087)
                     /locus_tag="PKU75_12100"
     CDS             complement(8685..>9087)
                     /locus_tag="PKU75_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81668.1"
                     /translation="KAGSDAYVDTDIWVIAQYCVYYETDDPSRVIGMGGIGFKAGTQV
                     TVTWSPGPNAPRTSSVGSSGDWEDQPHTVPCAADPMQLAVTGSTLDETSTGVLTAPCH
                     ATRHDGSGASARSVTAAGGAAEIVTAYGRRS"
BASE COUNT         1383 a         2997 c         3229 g         1378 t
ORIGIN      
        1 aggcggggca agtggccgac ccgcagcgtg ctctggaacg gatcgccggc ctgggccgga
       61 ccgccctcac tgagctcgac cggctcgtcg tgcacctgcg cgacccgcgg caggaagtcg
      121 ccgtctcagc gcccccgcgg ctgctcgata tcgatgagtt gctcgctgtc ccccttcgcg
      181 agtccggcgt ggacgtaaag gtgcgcatcg gcgacgggct cgacctcgcg gacgccgacg
      241 tgctcgccgt ctaccggatg acgcaggagg cgctcaccaa tattgcccga cacgccgggg
      301 cccggtgcgc gtgggtcgac ctgcagcgca ccggtgaccg agtgcggctg cgggtcagcg
      361 acgacggcgt cgggcccgcc acccgggcgg ccgagccggc ccggggactc ggactggtcg
      421 ggatcgcgga gcgggccgcg gcatacggcg gcacggccga gttgctctca cggcccggcg
      481 gcggatccac actggacgtc gtcctggtgg tgcccacggt gagcaggaga gcccggtgat
      541 ccgggtcgcc gtcgtcgacg accaggacct ggtccggcag ggcctggccc tgatcctgcg
      601 cgcccagccg gaccttgatg tggcggtgga ggcggccgac gggcaggagt gcctcgacgc
      661 cgtccgcgcc gacccgcgca tcgacgtggc actcgtcgac attcgaatgc cgggcctgga
      721 tgggctcgcg gccacccggg ccctggctgc cgtcccgcac gcgcccgccg tcatcgtcgt
      781 gacgaccttc gacgaggaca gctatgtcct cgacgccatc gccgccgggg ctcgcggctt
      841 cctgctcaaa cggtgctcgg gccgcgaact ggtcgaggcg gtgcgccggg tcgcggccgg
      901 ggactccatc ctcagcccgg acgtcaccgg cgcggtcctg gaccgggtgc gtgcgctcgg
      961 gccggcgacg ttggccgacc tgacaccctt cgcgctgacg gagcgggagc gcgagatcct
     1021 cgcgctcctc ggcgcagggc tgaccaatgc ggagatgacg gttcggctcc acctgtcgat
     1081 gagctcggtc aagacgcacg tgagcaatgt gctggccaag acgggaagcc gcgaccgggt
     1141 ccaggctgcc atcctcgcga tccgggccgg cctgtcgtag gccccgcccc gccgcggccc
     1201 cgtcgcccgt cgccggtgag aggggccggt agacttccag gagaacacgc gccgacctcc
     1261 ggcgcccgca caagtcgaag gccgagggcc cgcttccgtg accacgctgc atacgcccgc
     1321 cgccgtgccg acgcagccgt cgtggcggcg cacgctcgcg gcatacgttg cgctgaccaa
     1381 gccacggatc atcgaactgc tgctcgtgac aacggttccc acgatgttcc tcgcccagcg
     1441 cggggtgccg tcgctgtggc tcgtggtcgc cacgctcatc ggcggctccc tctccgccgg
     1501 ttcggcgaac acctacaaca tggtctacga ccgcgacatc gacgcgcaca tgggtcgcac
     1561 cgcgaaccgt ccgctcgtca ccggcgaggt cacgccgcga gccgccctga tcttcgcgac
     1621 cgcgctcgcg ctcatctcga cgatctggtt cgccgtcttc gtcaacggtc tctcggcggc
     1681 cttgtcgctc gccgcgatcg tgctgtatgc cgtgggctac acgatgatcc tcaagcgccg
     1741 aaccgcacag aacatcgtgt ggggcggcat cgccgggtgc atgccggtgc tcatcggatg
     1801 ggcggccgtc acgggcaccg tgtcgtgggc ggcggtcgtg ctcttcctcg tcatcttctt
     1861 ctggaccccg ccgcactact ggcccctgtc gatgcgattc aaggaggact acgcgcaggc
     1921 cggcgtgccg atgctgccgg tggtggaggg caatgtcggt gtcggccggc agatcgtcgc
     1981 ctattcgtgg gtgatggtgg tgacctcgct ggtgctgatc ccggtcgcgc cgatgggctg
     2041 gctgtatgcc gtggccgccg tcctgtccgg tgggctgttt ctcgcggagg cgcaccgtct
     2101 gctcaaggcg gcgaaggcgg atgcgtcgct ggcgcagttg aagccgatgc gcctgttcca
     2161 ttactccatc acctatgtga cggtgctctt cctcgccgtc gccatcgacc cgctcgtctt
     2221 tcacgccctg ccctggggtt gatgaccctc tgcgtcgcgt ggccgtgggc catgacgttg
     2281 gccacaatga cgcctcgatg gggggttgag ggggatctcg ttacatccgg tggcggataa
     2341 tggaggtggt ggccggaatg gctatatcgc ggggcgttgt ttggacggga tgtccacccg
     2401 tgatcgcgtt cgaggcgagc cggccgccgc gacaggagag aagaaatgaa tatccacacc
     2461 ggcccagact tcgtggcgtc ggaactgcac taccggctcg aaagcgccac cccgacaacg
     2521 acatcgccgg cgcgctccca ccgccggcgc gggctgcggg cgttgctgcg gcgcctcacc
     2581 acccctgagt accggccgtg gccggacggg gacggtccca cccaagttcc ctgatcccgc
     2641 cgacatgtgg tctccctgac ccctcggcgg cagcgcgccg aagaaaaggg tgatgccctc
     2701 gcggcgtcct cgggtagggt gctcccgtct cacctgatcg gcggaagggg gtgcgagatg
     2761 tcgaccacaa catcgatggc gcgcccccgt ctcacctgac cgagacatcg gactggtgcg
     2821 tgcctgcata ccaggagaag caccatgaag atcagagcga tcatgattga ttgcaccgac
     2881 cactatgccc agtgccgttg gtgggaaagc gttctcgacg actacgcgga cgcgcccgac
     2941 gacccgaact atcccgacga cgagacgatg acgctcgtcg ggccggaagc acagccgtgg
     3001 ctgtgcttcc aacgggtcga ggactccaag cgcaccaaga accggctgca cttcgacctg
     3061 catccactgg gccggacccg ggatgaggaa gtcgagcgcc tcctcgagct cggtgccacg
     3121 gtcgtcggcg accatcggcg cccgaacggc tccggctggg tgacgatggc cgacccggag
     3181 ggcaacgagt tctgcgtcga acgcagcgag gcggagatgg ccgccggcca gtagccgcaa
     3241 cggctccttg cgtcccgcgt gattgccgac gtgacactca cgcgggacca agggtctgcc
     3301 ccgccgtgct ttctcgcact tgcagggacc agggcacctc cacccgtatg gccggcccgg
     3361 ccccgccttc gatgcgccgg atcagcaggt cgagaatgct catggtcaag gcgccgagat
     3421 ccggtgcgat cgttgtcagg gtgggcgtgt ggtagcgccc ttcggggatg tcgtcgaagc
     3481 ccgcgatggc gatatcgtcc ggcacccgga ccccggcctc gtggcaggac tccgccaagg
     3541 tgcgcgaggc cttcatgagc ggcgactccc cggccgactt ccccgaggag cactacgagg
     3601 cggactggac cggccggttc accctggagc agctcaacgc cactggtcgc cgggggatgg
     3661 gcctggactg acgcaacagg tcagcggccg ttggcggaga agtgctgcca gtccttcagg
     3721 gaatgccagt cgccgcccca ggaccagccg acgccggcaa aggcccgcac cacgacgtca
     3781 ccggcatgga tcatgcccgt cgcctcctgg tcgcggtcga cgaagtcgcc ggccgtcgcg
     3841 ggatagacct tgccgtcctt gaccatcggg ttctcgagcg ggttgatgtc gatggcgcgg
     3901 ccgtatgcgt gctgggagca cgaggtcgtg ccttcgacga agcggccgtt gaaggcggag
     3961 ctgttgtcgt cccgcatcga catctcatcg tcggcgccgt agtcctcgac ggtgaccatc
     4021 ttgcggatcg ggaaacgcgc ttcgtagagc gcacccagag ccgagacgac ggcgtccacc
     4081 gcgtcctcgt tgaggacaag ttccccggta tgcggcgtct ggtcgaagcc ccagtagctc
     4141 acccgcacca ggcgcaggtc ggcgaacggg accgaacagg tgnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nncgcttgaa ctgcttgatc gcggcgacga gggcgacgac
     4321 caggaggctg gcgagcagca tgtgtgtcag gaccagcgcc tccggcagct tggtgaagta
     4381 ttgcgtgtag ccgacggcgc cctgtgcgag ggtcaccagg agcacggcgc gccaggcgtc
     4441 gcgcaggtgg ctcgagtggg acgagatgcg agtggcgacg accatggcca ccacgaggcc
     4501 gacgaagagc atgacggagt cggcgtgcag ccacgaggcc gagcgcgggt cgaaggacag
     4561 gcgcgccggc tcgtccgcgt caccggagtg cgggccggac cccgtcacga cggtgcccag
     4621 gaccaagacg cccaccgcga gtgccactgt ggcccagccc aattggcgga cgatctgggg
     4681 ggccgtatgc cgccggggac cgggcggcat accgctctcg acgaaagcgg ccatggccgc
     4741 ggccaccagg cccatggaca ccaggaagtg ggcggcgacc gtggcggggt tgaggccggt
     4801 gcggacggtg atgccgccga gcacggcctg gacgccgaca ccgaccagga cgagcagcgc
     4861 cgggacgagc aaggcgcggc ggcggtcggt ccacttccag atcgcgagga cggcggcgat
     4921 ggcgacgaag ccgaccaggc cggtcagggt gcggttgccg aattcgatgt acttgtggaa
     4981 gccctctgcc tgctcccgca cgggggtata cgagccgggc acgcactgcg gccagctcgg
     5041 gcagccgagg ccgctcccgg tgaggcggac caggccgccg gtgacgacga tgagcacctc
     5101 gacgatgacg ttgagcagga ggatgcgccg cagccacggc gtcgtcgtgc cccgatgctc
     5161 agcggtcaag cgcggtcgat cgtggtcgag cgtcgtcacg ggcccagcct acgctcggca
     5221 tacggttggc ccgaacggcc ctgctgccgt cgcgcggacg gatcactagg gtgagtggta
     5281 tgacgatcgt cgacccgacc acaaccaccg cctgggccac gctgacgggg ctgaagaccg
     5341 catacacacc cgatctgcgc gccgcgttcg cgggcgatcc ggagcgcgcc gcccgcttca
     5401 cgcgcacgtg cgccgacctc tacgtcgacc tgtccaagaa cctcgtcgat gaccacatcc
     5461 tcgaggccct cgtcgccctt gccgacgagg tcggcctggg cgaccggcgc gatgcgatgc
     5521 tgagcggcga gcggatcaat gtcaccgaga accggtcggt gctgcatacg gcgctgcgcc
     5581 ggcccgcagg ggactcgctg gtcgtcgacg gcaccgacgt gatgcccgag gtccacgagg
     5641 tgctggacaa gatctacgcg ttcgccgaca aggtccgcga cggggagtgg gtcggcgtca
     5701 cgggcaagcc gatcgagacg gtcgtcaata tcggcatcgg tggctcggac ctcggtccgg
     5761 tgatggtgta tgaggcgctg aaaccctatg tccacgaacg catccaatgt cggttcatct
     5821 ccaacatcga ccccaccgac tcgggggaga agctcagcga tctcgacccg gagaccacgc
     5881 tgttcatcgt cgcgtccaag accttcacga ccctggagac gctgacgaac gctcgcatgg
     5941 cacgcgattg gctgtggcgc cggctcatcg agcagggggc gatcgaggac accgaggagg
     6001 cgcggaaggg cgcggtcgcc aagcacttcg tggcggtctc gaccgcgctg gacaaggtcg
     6061 ccgacttcgg gatcgatccg gcgaacgcgt tcggcttctg ggactgggtg ggcggtcgct
     6121 attcggtcga ctcggcggtc ggcaccgtcg tctgcgtcgc catcggcaag gagaacttcg
     6181 ccgacttcct cggcggcttc cacgccgtcg acgagcactt cgccacggcg ccgtgggcgc
     6241 agaacgtgcc ggcgctgatg ggcttgctca acgtctggta tgtcaacttc ttcaaggccg
     6301 cgagccacgc ggtgctgccg tatgcgcagt accttcaccg cttcgccgcc tatctgcagc
     6361 agctgacgat ggagtccaac ggcaagggcg tgcgcgccga cggcgagccc gtcacgaccg
     6421 agacgggcga ggtcttctgg ggcgagccgg gcaccaacgg gcagcacgcc ttctatcagc
     6481 tgatccatca ggggacccag ctgatcccgt gcgacttcat cgcgatggcg aacacggcat
     6541 acccgacgaa ggacggcgac gccgacgtcc acgaactctt cctcgccaac ttcttcgccc
     6601 agaccaaggc gctcgcgttc ggcaagacgg ccgaggaagt gcgcgccgaa ggggttgcgg
     6661 aggaactggt ctcggccaag gtgttcgagg gcaaccggcc gacgacgtcg atcatggcgc
     6721 ccgccttgac cccgtcagtc gtcggtcagc tcatcgcgct ctacgagcac atcaccttca
     6781 cccagggcgt cgtgtggggc atcgactcct tcgaccaatg gggcgtcgaa ctcggcaagc
     6841 agctggcgat gcagatcgcc ccggcgatct cgaaggacga cgctgccctc gagcagcagg
     6901 acggttcgac gcgcgggctg atcgcgtact accgctccca gcggtcgtag cccccgaagg
     6961 gccggccttc ggatcgtccc ggacctgcca tgtcgcacgt tgggcgatgc gatctgccac
     7021 atcgcccagg ttgcgacctc caagcggaac tcgcgtcaca acctgggcga ttcgggtgcc
     7081 tgcatcgccc aacttgtgac accgcgctgg tgcggcgtca gcgagcggtc tgtcgctgcg
     7141 ggcgccgacg agcgcggttg ccggggttcg cggcgggctg gctctcacgg cgggcccgat
     7201 cggcccggcc cggccccggt gactgcgccg atcgcgacgg ctgaccggag cgcggttgcc
     7261 gggcgcggcc cggggacggt gccgctggcg gcgtggcggc atagccaccg gggaaatccc
     7321 ttgtgccagg ggcgagttgg cgaagcaacg ggtgggccgg gttgacgcgc gtgatggtcg
     7381 ggcggatgcc ggccttgcga gtcagatccc ggacgtcgcg gacctggtca tcgagcatca
     7441 tcgtcacgac cgtgccggag ttgcccgcgc gggcggtgcg gccggagcgg tgcaggtagg
     7501 ccttgtgctc caccggcggg tcggcatgga tgacgagcgc gacatcgtcg acatggatgc
     7561 cgcgggcggc gatgtcggtg gccacgaggg tgccggcagc gccgctgtgg aaggcgtcca
     7621 tcgtgcgggt ccgggcgttc tgcgacaggt tgccgtgcaa ctcgaccgcc gggacaccgg
     7681 ccgcattgag ttgccgcgca agctttttgg cgcggtgctt ggtgcgggtg aagacgatcg
     7741 tgcggccggg cgcggcggtc aggtcgagca ggaccggcag gtggtgggct gggccgacgt
     7801 gcagcacgtg gtggtccatc tcggccaccg ggctctgagc ggagtcggct tcgtgagtga
     7861 ccggccggct cagatagcgc ttggcgagga cgtcgacgcc ggcgtccaag gttgcggaga
     7921 agagcatgcg ctggccctcg cgaggggtgc ggtcgaggag acggcgcacg acgggcagga
     7981 agccgagatc ggccatgtgg tcggcctcgt cgaggaccgt gacctcgacg gcgtcgaggc
     8041 tgaggtggcc ctggccgagg agatcctcga gtcggcccgg gcacgcgatc acgatgtcga
     8101 cgcccttgcg aagggccgcg acctgggggt tctgcccgac gccgccgaag acggtgcggg
     8161 agttcaggcc cgttgccgcg gccagcggcg cgagggactc ctcgatctgc gcgaccagtt
     8221 cacgggtggg ggcgaggatc agcgcgcggg ggcgcttcgg catacggcgc gatggggcgg
     8281 aggcgagtcg ggcgacgagg ggcaggagga acgcgtaggt cttgcccgag cccgtgcggc
     8341 cgcgaccgag gacgtctcgg cccgcgagag agtccgggag cgtcgcggcc tggatgggag
     8401 tgggggtggt gatgccacgc tgcgcgaggg cggcggtcag cttggcgggc acgccgagcg
     8461 tgccgaaagt cgtgtcggtg gacacagaaa tggtgctcca atgagtctcg aagagtcgtt
     8521 ctgcccggcg cgagcgtcgt tggtgtgatg taaccggctc gcagcagcac tggcgaatcg
     8581 acaagcgaag cggcccgcgg cagaactggt cgggcctatc tacccattct accgtgcccg
     8641 gccggccgcc tcttccaagg ggcggccggc ccgatacgtg gccttcagga gcgtcggccg
     8701 tatgccgtga cgatctctgc cgccccgccg gcggcagtga ccgagcgcgc ggaggcgccg
     8761 gagccgtcgt gacgggtcgc gtggcagggg gccgtcagca cgccggtgga cgtctcgtcg
     8821 agggtcgagc ccgtgacggc caactgcatg ggatcggcgg cgcacggcac ggtgtgcggc
     8881 tgatcctccc aatcaccact cgacccgacg gacgaggtcc gtggtgcgtt cgggcccggc
     8941 gaccaggtga ccgtcacctg agtaccggcc ttgaacccga tgccccccat cccgatcacc
     9001 ctgctcgggt cgtcggtctc gtagtagacg cagtactgcg cgatgaccca gatgtcggtg
     9061 tcgacgtagg cgtccgagcc cgccttc
//
LOCUS       DAOPJG010000141         8949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8237_length_8949_cov_8.536879, whole genome shotgun sequence.
ACCESSION   DAOPJG010000141 DAOPJG010000000
VERSION     DAOPJG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8237_length_8949_cov_8.536879"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKU75_12105"
     CDS             <1..408
                     /locus_tag="PKU75_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HPF81669.1"
                     /translation="VTVMVLTETRLVIAHADDHTDDPHGLEDVATATTECIPLRFVRG
                     IMLTHVVPDPRHYVPGTLGRELTLTLGWGTVSRVDLLPATCGDPACDADHGFDGTIAS
                     DDLVLRISADADGNRPLGQAREFAAALSARIGR"
     gene            420..1598
                     /locus_tag="PKU75_12110"
     CDS             420..1598
                     /locus_tag="PKU75_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPF81670.1"
                     /translation="MSGADAPRPPDRPPGLHPPSYRHTSLPQVLPAVAASLGYAVGEG
                     RAGDLTLAPARSAVVVLLDGLGLELLRRRAGHAPFLRSLLPTGTELACGFPATTATSL
                     ASLGTGRAPGEHGLLGYQVAIPETGQIFDHLSWENGPDPVAWQPDPTIFERLAAEDVA
                     VVRIGLPRFDGSGLTTAAQRGGRFVGANSLSAGIRAAVRAVTTNDRALVYLYWGDIDR
                     TGHMLGVGSPEWTAVLEDADAQLRRLAEALPRHTCLTITADHGMIDVPFEERIDLATD
                     PELDAGVRAITHEPRAPHVYCEPGAAAAVAEAWRGRLAGRAVVLTREQAIDEGWFGPV
                     ARRNAGRIGDLVVAMGPRGAIEDSRTSKPILLQLLGMHGSVTRDEVAVPLLHRPAESD
                     "
     gene            1627..2301
                     /locus_tag="PKU75_12115"
     CDS             1627..2301
                     /locus_tag="PKU75_12115"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPF81671.1"
                     /translation="MAELVFFSGTMDCGKSTLALQMDHNHRARGRHGLIFTRLDRAGE
                     AILSSRLGLATPAIEVTPELDLWEVVVTHLMGGQKVDFVICDEAQFYAPEQIEHLARV
                     VDELDIDVFAFGITADFRTQLFPGSRRLIELADRVQVLQVEALCWCGRRATHNARIVD
                     GVMVVEGEQVVVGDTDGGASGLVEYEVLCRRHFMRRMNAKAAQAAALSADVLPLELDA
                     CPIPPC"
     gene            complement(2465..3799)
                     /gene="sepH"
                     /locus_tag="PKU75_12120"
     CDS             complement(2465..3799)
                     /gene="sepH"
                     /locus_tag="PKU75_12120"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HPF81672.1"
                     /translation="MQELRLFGVQEDGRFVLLAAADGTRYRLPLDEDLRVAARRARTP
                     SGASESASLTARDVQALVRAGHDVEEVAERAGWPVSKVNVFAAPVLAERAHVAGLAQG
                     MRPRAGDPELLGSRVEKRLAGRGVDLAGAEWDSSRDDSGAWHVHVDFAAGGRERRASW
                     RFDPATEHLEAEDDEARWLSEDEDHGPVTPQVHRYGGEQVFDVEKTGGVVEEEDENAV
                     KADLPRSDQTDALMTAIRAHSHAGDRRGRRRRATRTPKAPAPAPASVPAPEEDREPEV
                     VREVGFGAPVTQDGPDAAAVEAAAGEPGGGTGTAGGRLQGADELPFAEFGHPEPAAGP
                     AAAGPVASVAAVPTTARAAEAADDPDLVDFPDSDEIPPPARGTHPNDVAAEPEPQPEP
                     EPEPEPEPEPEPAASKPAPPKSGGARRKGRVGVPNWNDVMFGPSRGSTPDSE"
     gene            3932..5026
                     /locus_tag="PKU75_12125"
     CDS             3932..5026
                     /locus_tag="PKU75_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HPF81673.1"
                     /translation="MPAAPDADSPLAPFRSVVVLGFGGPEAPEEVMPFLRRVTAGRGV
                     PDERLAIVAEHYHQFGGRSPINDQNRALGAALAAELADRGAPVAVRLANRNSPPYAAD
                     VLPELAPGPVLVLLTSPWRSYSSCRQYREDLAAAGAGVTLAKLPPYAALPGFRSAVTR
                     CTLEALDAALAADSQGPLALVCIAHSIPTAADAAAGRPGEGGHAYSRELRDLADEVTQ
                     AAARHTGLDLATDLAWCSRSGPPQQPWLEPDILDRLTELAADGIRSVVLAPIGFLSDH
                     MEVVYDLDTEAAAKAAELGIRLHRAPTVGTDPEFVAGLADLALARAAAARGDDAALAA
                     YPPADCPPDCCRGRGPRPALCGPEDGSDAP"
     gene            5016..5870
                     /locus_tag="PKU75_12130"
     CDS             5016..5870
                     /locus_tag="PKU75_12130"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPF81674.1"
                     /translation="MLPDLPIPPLATLVELERVALHVATEAALLIVDRSAATVAATKS
                     SPTDVVTEMDRRSQELVQTLLRAERPQDGFFGEEDGRAAGTSEITWVVDPIDGTVNYV
                     YDIPAYAVSVAAVVGDPQTPGAWRPVAGAVVNPVTGERFWARAGGGAWRQRGRFDPIA
                     VHASGQADPAASLVATGFSYDADTRAWQAAVLSEVLPAVRDIRRGGSAALDLCHVADA
                     TLDAYYEHGLNPWDFGAGWLIVEEAGGAVAGPSGQAPDRPMTIAAGAGFGALSELVRR
                     ALEAADRG"
     gene            6020..6319
                     /locus_tag="PKU75_12135"
     CDS             6020..6319
                     /locus_tag="PKU75_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HPF81675.1"
                     /translation="MATDYDAPRKTDDELNEDSLEELKARRVDKSTSSVDVDETEQAE
                     GFELPGADLSGEELSVRVIPRQADEFTCSRCFLVHHRSQLASDKGGVLVCNECAA"
     gene            6378..6821
                     /gene="dut"
                     /locus_tag="PKU75_12140"
     CDS             6378..6821
                     /gene="dut"
                     /locus_tag="PKU75_12140"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPF81676.1"
                     /translation="MLVRTLDPAATLPAYARDGDAGADLACIEERTLAPGERALVRTG
                     IAVAIPDGWVGLVHPRSGLAARHGITIVNAPGTVDAGYRGEILVNLLNTDPRESFTIT
                     PGDRIAQLVLQEVARAHFTAADSLPESSRGETGHGSTGGFGTSAT"
     gene            6852..7670
                     /locus_tag="PKU75_12145"
     CDS             6852..7670
                     /locus_tag="PKU75_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HPF81677.1"
                     /translation="MSIFRRKSSEPARDESGSPEVVAPDDAALDEMASGEAAAGDLVE
                     EFDDEPEVSDRSQGPWDRSEVDGLGDRLDLGAVWIAPLDGAELRLEVDAATDTVSAIQ
                     LVIPQTSSAAQIQAFAAPRSGGVWRDVRTEIADAITAAGGTAETVDGVFGPELNVRMP
                     QAGPDGRTVFAPARFVGVDGPRWFLRAVLSGQAAVEDDAAAQLIEGIRSLVVVRGSDP
                     MAPREMLALTLPEAPAEPEPEAAVSEEDADGPDERYTNPDLNPFERGPEITEVR"
     gene            7674..8108
                     /locus_tag="PKU75_12150"
     CDS             7674..8108
                     /locus_tag="PKU75_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPF81678.1"
                     /translation="MAGARAGSPHRLGAPGARSGSWGGLLRRLGERMTRPADQRDAEE
                     LKEHARAHGAAAIDTLADRQIATVSGTIRAVTLRPRSTVPALVVELYDGSQTLVLIWL
                     GRRQIRGIEPGAYVRATGRVCIREGVPTIFNPAYELLPRTGR"
     gene            8108..8812
                     /locus_tag="PKU75_12155"
     CDS             8108..8812
                     /locus_tag="PKU75_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HPF81679.1"
                     /translation="MTNAEDAAQEARAATVEEIIRHRLSAAVGGWRGSLETALPTIAF
                     VIGWTVTTDVRTSVIAAGAVAVLLGVARIVQRQSLQYVLSAVFATAIAAFFALRSGKA
                     EDAFLPGIITNAAYAVGTLLSVVTRWPLVGFLVGAGDPLAKEDPLRWRRDPSMVAVCG
                     RLTLIMTAMFALRVAIMYPLYKAGNVAGLGTAKVVLGWPLWVAALALMGALLVRGHTP
                     HTNPEPETQGTSASRG"
BASE COUNT         1180 a         3388 c         3091 g         1290 t
ORIGIN      
        1 gtcacggtca tggtcctcac cgagacccgc ctggtcatcg cccacgccga cgatcacacg
       61 gacgacccgc acggactgga ggatgtcgcg acggcgacca cggagtgcat cccgctgcgc
      121 ttcgtgcgcg gcatcatgct cacgcacgtc gtgcccgacc cacgccacta cgtccccggc
      181 acgctcggtc gcgaactgac cctgaccctc ggctggggca ccgtctcccg cgtcgacctg
      241 ctgcccgcga catgcggcga cccggcctgc gacgccgacc acggcttcga cggcaccatc
      301 gcctccgacg acctggtcct gcgcatctcg gcggacgccg acggcaaccg cccgctcggc
      361 caggcgcggg agttcgccgc ggcactgtct gcgcggatcg ggcgctgacc ctcccgacga
      421 tgtccggagc cgacgccccc cgaccgcccg accgcccccc ggggctgcac ccgccgagct
      481 accggcatac cagcctcccg caggtgcttc cggccgtcgc ggcctcgctg gggtatgccg
      541 tgggggaggg ccgcgccggt gacctcaccc tggcgcctgc gcgcagcgcc gtcgtcgtgc
      601 tcctcgacgg gctcgggctg gaactgctgc ggcgacgtgc ggggcacgcg cccttcctcc
      661 ggtcgctgct gccgaccggg accgaactcg cctgcggctt cccggccacc acggcgacct
      721 ccctcgcctc cctcggcacc ggacgcgcgc ctggcgagca cggcctgctg ggctatcagg
      781 tggcgatccc ggagacgggg cagatctttg accacctctc gtgggagaac gggcctgatc
      841 ctgttgcgtg gcaacctgat ccgacgatct tcgagcggct cgcggcggag gatgtcgcgg
      901 tcgtgcgcat cgggctgccg cggttcgacg gttccgggct gacgacggcc gcccagcgcg
      961 gcggccggtt cgtcggagcg aactcgctgt cggccggcat acgcgccgct gtgcgggcgg
     1021 tgacgacgaa cgatcgcgcc ctggtctatc tgtactgggg cgacatcgac cgcaccggcc
     1081 acatgctcgg agtcggttcg ccggagtgga cggcggtgct cgaggacgcc gacgcccagt
     1141 tgcgccggct tgcggaagcc ctgccgcggc atacctgcct gacgatcacc gcggaccacg
     1201 gcatgatcga cgtgccgttc gaggagcgga tcgatctggc gaccgacccc gaactggacg
     1261 ccggcgtgcg ggccatcacg cacgagcctc gcgcgccgca cgtctactgc gagcccggcg
     1321 ccgccgccgc cgtggccgag gcctggcggg gccggctcgc cgggcgcgct gtcgtgctca
     1381 cgcgcgagca ggcgatcgac gagggctggt tcggtcctgt ggcgcgccgc aatgccgggc
     1441 ggatcggtga cctcgtggtg gccatgggac cgcgcggggc gatcgaggac tcccgcacct
     1501 ccaagccgat cctgttgcag ctgttgggaa tgcacggctc ggtcacgcgg gacgaggtcg
     1561 ccgtgccgtt gctgcaccgg cccgccgagt cggactgaca cgcgcgatct cttaagcttc
     1621 tccctcgtgg ccgaactcgt cttcttctcc ggaaccatgg actgcgggaa gtcgaccctg
     1681 gccctgcaga tggaccacaa ccaccgagcc cgcggccgcc acggactgat cttcacccgc
     1741 ctcgaccgcg ccggcgaagc catcctctcc tcgcgcctcg ggctggccac ccccgccatc
     1801 gaggtcaccc ccgaactcga cctctgggag gtcgtcgtca cccacctcat gggcggccag
     1861 aaggtcgact tcgtcatctg cgacgaggcc cagttctatg cgcccgagca gatcgaacac
     1921 ctcgcccgcg tcgtcgacga actcgacatc gacgtcttcg ccttcggtat caccgccgac
     1981 ttccgcacgc agctcttccc cggttcccgg cgcctgatcg aactggccga ccgggtgcaa
     2041 gtgctgcagg tcgaggccct gtgctggtgc ggccgccgcg ccacccacaa cgcgcgcatc
     2101 gtcgacggcg tcatggtcgt cgaaggcgaa caggtcgtcg tcggcgacac cgacggcggc
     2161 gcctccggcc tcgtcgaata tgaggtcctc tgccggcgcc acttcatgcg ccgcatgaac
     2221 gccaaggccg cccaggccgc cgccctctcc gccgacgtgc tgcccctcga actggacgcc
     2281 tgccccatcc cgccctgctg aacgggcgtc gagatgtcga ttcccgggcg tcgagatgtc
     2341 gatgccgggc gtcgcaactg cgcaccagcc cgccgctact ggtagccacg gggcctaccc
     2401 attggtactg gttgcgcgca cgacgactat cgatgggtaa ccccgtcggt atgccgaggg
     2461 ccggctattc gctgtcgggg gtgctgccgc ggctggggcc gaacatgacg tcgttccagt
     2521 tggggacgcc gacgcggccc ttgcgcctgg cccctcctga cttgggcgga gcgggcttcg
     2581 aggcagcggg ctcgggctcg ggttcgggtt cgggttccgg ctcgggctcc ggctgcggct
     2641 ccggttcggc ggcgacgtcg ttggggtggg tgccacgggc gggcggtggg atctcgtcgc
     2701 tgtcggggaa gtcgacgagg tccggatcgt ccgcggcctc ggcggcgcgt gctgtggtgg
     2761 gcaccgcggc caccgatgcc acggggccgg ctgccgcggg gcccgctgcc ggttcggggt
     2821 ggccgaattc cgcgaagggc agttcgtcag ccccctgcaa ccggccgccg gccgtgccgg
     2881 tgccgccgcc cggttcccct gcggcggctt ccactgcggc ggcgtccggc ccgtcctgcg
     2941 tgaccggcgc cccgaacccg acctcgcgca cgacctcggg ctcgcggtcc tcctcgggtg
     3001 ccggcacgga agccggtgcc ggggcgggag ccttgggggt gcgggtggcg cggcggcgtc
     3061 ggccgcggcg gtcaccggcg tggctgtggg cgcggatggc cgtcatcagg gcgtcggtct
     3121 ggtcgctgcg gggcaggtcc gccttgacgg cgttctcgtc ctcctcctcg accaccccac
     3181 cggtcttctc gacgtcgaag acctgctcgc cgccgtagcg gtgcacctgc ggggtcaccg
     3241 gcccgtggtc ctcgtcctcg gagagccaac gcgcctcgtc gtcctcggcc tccagatgct
     3301 cggtggcggg gtcgaagcgc cacgacgccc gccgctcccg gccaccggca gcgaagtcga
     3361 cgtgcacatg ccaggcgccg gaatcgtctc tgctggaatc ccattcggcc cccgcgaggt
     3421 ccacgccccg cccggccagt cgcttctcca cacgcgaacc cagcagttcg ggatcgccgg
     3481 ctcggggccg cataccctgg gccaacccgg ccacatgcgc gcgctcggcg agcacgggcg
     3541 cggcgaaaac gttgaccttc gataccggcc agccggcccg ctcggcgacc tcctcgacgt
     3601 cgtgcccggc gcgcaccagc gcctggacgt cccgggccgt caaggacgcc gactcgctcg
     3661 ccccgctggg ggtgcgggcg cggcgggccg cgacgcgcag atcctcatcc aggggcaacc
     3721 ggtagcgggt gccgtccgcc gcggcgagca gcacgaaccg gccgtcctcc tggacgccga
     3781 agagacgcag ctcttgcata cggtttgcct ctccaccgaa attcgacgat caatgcccca
     3841 cagatgctgc cacgtcacgc cgctctgccg cggccaggcc acgccgcggg cgacgtcgta
     3901 agtgccccgc cggcgcttgt acggtggtgc ggtgcccgcc gcccccgacg ccgactcacc
     3961 gctcgcgccc ttccgcagcg tcgtggtgct cggcttcggc ggcccggagg ctccggagga
     4021 ggtcatgccc ttcctgcgcc gggtgacggc cggccggggt gtccccgacg agcggctggc
     4081 gatcgtggcc gagcactatc accagttcgg tgggcggagc ccgatcaacg accagaaccg
     4141 cgcgctcggc gccgccctcg ccgccgagct ggccgatcgt ggtgcaccgg tggccgttcg
     4201 gctggccaac cgcaacagcc cgccgtatgc cgcggacgtc ctccccgagc tcgctcccgg
     4261 gcccgtgctg gtgctgctga ccagtccgtg gcggtcctac tcctcgtgcc gccagtaccg
     4321 cgaggatctc gcggccgccg gcgccggggt gaccctcgcc aagcttccgc cgtatgccgc
     4381 cctccccggc ttccgctccg ccgtcacccg ctgcaccctg gaggcgctcg acgccgcgct
     4441 cgccgcggac tcgcaggggc cgctcgccct ggtgtgcatc gcccactcca tccccaccgc
     4501 cgccgacgcg gcggccggaa ggccggggga gggcggccac gcctactccc gggagctgcg
     4561 cgacctggcg gacgaggtga ctcaggcggc ggcgcggcat accggcctcg acctggccac
     4621 cgacctggcc tggtgctcga ggtccggccc gccccagcag ccctggctcg aacccgacat
     4681 cctcgaccgg ctcaccgagc tggcggcaga cggcatacgc agcgtcgtgc tcgcgccgat
     4741 cgggttcctc tccgaccaca tggaggtcgt ctacgacctc gacaccgaag cagcggcgaa
     4801 ggccgctgag ctcggcatcc ggctgcaccg cgccccgacc gtcggcaccg accccgagtt
     4861 cgtcgcgggg ctcgccgacc tcgccctggc ccgggcggcg gcggcgcgcg gcgacgatgc
     4921 ggcgctcgcg gcatacccac ccgccgactg cccacctgat tgttgccggg gccgcggccc
     4981 gcgcccggcc ctgtgcggac ccgaggacgg ctccgatgct ccctgacctg cccatcccgc
     5041 ccttggccac cctcgtcgaa ctggagcgcg tggcgctgca cgtcgcgacg gaggcggcgc
     5101 tgctgatcgt cgaccggtcg gccgccacgg tcgcggccac caagtcctcc ccgaccgacg
     5161 tggtcaccga gatggaccgg cgcagccagg aactcgtcca gaccctgttg cgcgccgaac
     5221 gcccgcagga cggcttcttc ggggaggagg acggccgcgc ggcggggacc agcgagatca
     5281 cgtgggtcgt cgacccgatc gacgggaccg tcaactatgt ctacgacatc ccggcgtatg
     5341 ccgtgtccgt cgccgccgtc gtcggcgacc cgcagacccc gggcgcctgg cggccggtcg
     5401 ccggcgcggt cgtcaatccc gtgacgggag aacggttctg ggcgcgcgcc ggtggcggtg
     5461 cgtggcggca gcgggggcgg ttcgacccga tcgcggtcca cgcctcaggg caggccgatc
     5521 ccgcggcctc cctcgtcgcg acggggttct cgtatgacgc ggacacgcgg gcttggcagg
     5581 cggcagtcct cagcgaggtg ttgccggccg tccgggacat ccggcgcggc ggcagcgccg
     5641 cgctcgacct gtgccatgtc gccgacgcca ccctcgacgc ctactacgaa cacggtctca
     5701 acccgtggga tttcggggcg ggctggctga tcgtcgagga ggccggcgga gcggtcgccg
     5761 ggccctccgg gcaggctccc gaccggccca tgaccattgc tgccggagcg ggattcggcg
     5821 ccctgtccga gctggtgcgc cgggcgctcg aggcggccga ccggggctag ggcggcggct
     5881 gcggaatttc cgcgtgacat tggcgcttaa gtagggaaca atctgcgccc gcgcgggatc
     5941 tttgacgacg ccagctgcgt tccactcgca gcggactgtg taacgactgc gggacggaac
     6001 gggaaaggga cttttaggca tggcaaccga ctacgacgca ccacgcaaga ccgacgacga
     6061 actgaacgag gattcgctcg aggaactcaa ggcccgccgg gtcgacaaga gcacctcgag
     6121 cgtcgatgtc gacgagaccg agcaggccga gggcttcgaa ctgcccgggg ccgacctgtc
     6181 cggtgaagaa ctctccgtgc gggtcatccc gcgacaggcc gacgagttca cctgctcgcg
     6241 ttgcttcctc gtccaccacc gcagccagct cgcctccgac aagggcgggg tgctggtgtg
     6301 caacgagtgc gccgcctagg ccgctcccag cggtgaatgg ccaggcggac gcatccgcac
     6361 gaggcgaccg gccggtcgtg ctcgtgcgga cactcgaccc tgccgcgacc ctcccggcgt
     6421 acgcgcggga cggcgacgcc ggcgccgacc tggcctgtat cgaagagcgc acgctcgccc
     6481 cgggggagcg ggccctggtg cgcaccggca tcgccgtcgc catccccgac ggctgggtgg
     6541 ggctggtcca cccccgctcc ggcctcgccg ccaggcacgg catcaccatc gtcaacgccc
     6601 ccggcaccgt cgatgccggc tatcgcggcg agatcctcgt caacctgctg aacaccgatc
     6661 cgcgcgagtc cttcacgatc accccaggcg atcgcatcgc ccagctcgtg ctccaggagg
     6721 tggcccgcgc gcacttcacg gccgccgatt cgcttccgga gtcgagtcgg ggagagactg
     6781 gacacggatc caccggcggc ttcggcacga gcgccaccta gacacgaccc accacgaagg
     6841 accgacccac cgtgagcatc ttccgccgca aatcgagcga gccggcccgc gacgagagcg
     6901 gctcgcccga agtcgttgcg cccgacgatg ccgcgctcga cgagatggcg tccggcgagg
     6961 cggccgcggg tgacctcgtc gaggagttcg acgacgagcc cgaggtcagc gaccgctccc
     7021 aggggccgtg ggatcgcagc gaggtcgacg gcctcggcga ccggctcgac ctcggcgcgg
     7081 tctggatcgc gcccctcgac ggcgccgaac tgcgcctcga ggtcgacgcg gccaccgaca
     7141 ccgtctcggc catccaactc gtcatcccgc agacctcctc ggccgcccag atccaggcct
     7201 tcgcggcccc gcgctccggt ggggtatggc gcgacgtgcg caccgagatc gccgacgcca
     7261 tcaccgcggc gggcggcacg gccgagaccg tcgacggggt cttcgggccc gagctgaatg
     7321 tccgtatgcc gcaggccggc ccggacggtc gcaccgtgtt cgcgccggcc cggttcgtcg
     7381 gcgtcgatgg gccccgctgg ttcctgcggg cggtgctgtc ggggcaggcc gccgtcgagg
     7441 acgacgccgc agcccaactc atcgagggca tccgctcgct cgtggtggtg cgcggctccg
     7501 acccgatggc gccgcgcgag atgctcgccc tcaccctgcc cgaggccccc gccgagcccg
     7561 aacccgaggc ggccgtctcg gaggaggacg ccgacggtcc cgacgagcgg tacaccaacc
     7621 ctgacctcaa cccgttcgaa cgcggtcccg agatcaccga ggtgcgctga ccaatggccg
     7681 gtgctcgtgc ggggtccccg caccgtctcg gcgcgcccgg ggcgcgcagc ggctcgtggg
     7741 gtggactgct gcgccggctc ggcgagcgca tgacccgtcc cgccgaccag cgcgacgccg
     7801 aagagctcaa ggaacatgcc cgcgcccacg gggccgcggc gatcgacacc ctcgccgacc
     7861 ggcagatcgc gacggtctcc gggaccatcc gcgccgtgac gctgcgtccg cgcagcacgg
     7921 tcccagctct ggtcgtcgag ttgtatgacg gcagccagac cctcgtgctc atctggctcg
     7981 ggcggcgcca gatccgcggg atcgaaccgg gcgcctatgt gcgcgccacc ggccgcgtgt
     8041 gcatccgcga aggggtgccg acgatcttca acccggccta cgagctgctg ccccggacgg
     8101 gacgctgatg acgaacgccg aggacgctgc gcaggaggcg cgtgccgcca ccgtcgagga
     8161 gatcatccgc caccgcctct cggcggccgt gggcggctgg cgcggttcgc tcgaaacggc
     8221 tttgcccaca atcgctttcg tcatcggctg gaccgtcacg acggacgtgc gcacgagcgt
     8281 gatcgcagcc ggcgcggtgg ccgtcctgct cggggtcgcc cgcatcgtgc agcggcagag
     8341 cctgcagtat gtcctctccg ccgtcttcgc caccgccatc gcagcgttct tcgcgctgcg
     8401 cagcggcaag gccgaggacg ccttcctgcc gggcatcatc accaatgctg cgtatgccgt
     8461 cggcaccctc ctttccgtcg tcacccgctg gccgctcgtg ggttttctgg tgggagcggg
     8521 cgacccgctc gcgaaggagg acccgctgcg ctggcgccgc gacccgtcga tggtggcggt
     8581 ctgcggccgg ctcaccctga tcatgaccgc gatgttcgcg ctgcgggtcg ccatcatgta
     8641 ccccctgtac aaggccggca atgtcgctgg cctcggcacg gccaaggtcg tcctcggctg
     8701 gcccctatgg gtcgccgccc tcgccctcat gggcgccctc ctcgtccgcg gccacacccc
     8761 ccacaccaac ccggaaccgg aaacccaagg aacctctgct agcagaggtt agtcaaatca
     8821 gcacaccttg ctaggtctgc tgatttcact aacttctgct agcagaggtt cctcggcggg
     8881 gccgaagtgc cttggggggc agggggttcg gcgagctagg cgagttcccg cgggcgcttg
     8941 atgcgctgg
//
LOCUS       DAOPJG010000142         8945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8247_length_8945_cov_8.283127, whole genome shotgun sequence.
ACCESSION   DAOPJG010000142 DAOPJG010000000
VERSION     DAOPJG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8247_length_8945_cov_8.283127"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="PKU75_12160"
     CDS             <1..1399
                     /locus_tag="PKU75_12160"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPF81680.1"
                     /translation="IAALARGARVDEPWLPDLGVSLTFGADQISGPLLILTAAISVLV
                     VLHGQRHLPPGGPPALYFGLLLIVETGALATFWTGDLVVFFVAFEIVLIPMWVLIHRF
                     GDNHDPAARREAALRFVLFTALGSSLMLAGILGLLAQAGSSSMATVATVTIPSGTSLG
                     LALLLVIGLGVKVPLFPLHSWLPPAHTIAPTGGSVLLAAILLKMGTYGLIRLPVALTP
                     AGFARLAPVLAAFGVAGIVWGGLVCLIERDLKRLIAYSSVAHMGFVALALGTNSALGL
                     QAALLANIAHGVIAALLFFVVGGLKERWGSVDLAVVRPALRETWPGLGFALITGLAGS
                     LALPGLASFWGEFLALTAAWNPAYGTAAPYAVWGPAEPGLVQPGSGWDAGWVAWVPPT
                     GFLRTLTIVAAFGAVLAAAYSLRVARLVWAGDAAPRDRAGDLTWAEWVVATVLMLAIV
                     TLGLVPYVVYPGVGS"
     gene            1396..2889
                     /locus_tag="PKU75_12165"
     CDS             1396..2889
                     /locus_tag="PKU75_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPF81681.1"
                     /translation="MTTSLVSLDWLVLAPVLAPAFGVLLVLLADALAPRARGLHLPIA
                     LVAVGAGLGALPYAATSAVPARSLCRPGPEARCFYETAPWGLALQFLVLAGALLVLLM
                     LGPGTTHLDRTQRGGPAVTVALVLAATTGAAMVPATRDLPTWLIAIELATLPAIALVA
                     LSRGRGAESGALSLLMVSLFSFALLAVGVGLWTLATGDATFGVDTLRVAFADPDRHRV
                     LLLAILFLIAGLGFKLSAVPFHLWTPQAFVVADEPISAFLATVSKAAALGAAIVMLSP
                     LAALNASGDSGATSVRLVIGVLAIASMTLGNLIALRAQDPLRLLAWSTIAQAGWVLAP
                     LAMLTPTAASASVAYLLAYAAGTLAVFAVAAAVHSGTEGYGERSLTAYAGLGRGNPVL
                     AAILALGLLTLAGLPPAVLGVVAKVVALQPVVGAGQWWLVAAAILNIVLGIAVYLRWL
                     LIVVRPAGDAVPQRLRLDPGLAVALALLGAGLIVLSFAPGLVIGLTR"
     gene            3055..3921
                     /gene="htpX"
                     /locus_tag="PKU75_12170"
     CDS             3055..3921
                     /gene="htpX"
                     /locus_tag="PKU75_12170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPF81682.1"
                     /translation="MHNHFNGLKTAGLFGLIWLLLLGLGAVIGSGRYIWLFALIGVAT
                     TFYAYWNSDKIAIRAMKAYPVSEAQAPEMYRIVRELSTKAGKPMPQLFISPTAAPNAF
                     ATGRNPSHAAVCCTEGILQLLDERELRGVLGHELMHVYNRDILTGSVAAAIAGIITSV
                     AQMAMFFGGGSDEDRPNPLALLGMALLAPFAAMLIQMAITRTREYDADEDGAKLTGDP
                     LALASALRKLETGTRRAPLAPTPQVVNASHMMIANPFRAQDVSRFFATHPPMADRIAR
                     LEAMAGGIDRRR"
     gene            3977..6451
                     /gene="pepN"
                     /locus_tag="PKU75_12175"
     CDS             3977..6451
                     /gene="pepN"
                     /locus_tag="PKU75_12175"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HPF81683.1"
                     /translation="MSPLTRTEAQQRATTIRVSRMVVALDLDQGPETFGSRAEIHFEA
                     TAPETFVDLRPVGVARLELNGQDLDPAALVDGRLALTGLEPSNVLIVEATMAYSRDGQ
                     GLHRSVDPADEEAYVYGHLFLDAAPTVFACFDQPDIKAPYAVSVRAPADWRVLGNGAA
                     TRVAEGCWELAETPPLATYFVTVCAGPWASVEREHDGIRLGVHSRRSLSEALERQADS
                     LLNATADFFDYYHELFGIRYPFGDYHQVFCPEFNAGAMENPGCVTIRDTYLFRGSATH
                     DELLTRTNTIAHEMAHMWFGDLVTMTWWDDLWLNESFAEYLAHRACVAASEYADAWVD
                     STMARKLWGHSAERSPSSHPVAGSPAQDAQTALNNFDGISYAKGSAVLRQLIAHIGDD
                     AFVAGLRDHLQRNSFGNADLAGFLAAIERESGSDLSGWSHAWLETAGIDTLHVHQDTG
                     RVTRSTPDRFAKVSRPHTFDIAGFTSGTAVFRVTGTIDRATRKWPELLAADRAEVVIP
                     NASDLTWAVSGLEPESLANLPDALASIPDAQSRAVAWLALIDGMHEARIDPRLLMRTL
                     TMAWPLESNGSILGRVGNHVITRAIPVFLPPTEQPGGEEAMATAARRLYDGATSADHR
                     LIAARLLAATSSDETLLRSWLDGIDLPPELVDDSDFRWFVVRAMSRRGLLDEQGIESF
                     RRRDESLAGQQAALAARALRPTAEAKEWAWSELTGNPARSNYELNALAGAFWQTTDRD
                     LVSPYVERYFTDVPGLAGRLGEDALGTVAALAYPLPLVASRTLDLSRAALARDTLSPA
                     VRRSIVDAQAKLEEGLASRAAFPATG"
     gene            complement(6441..7421)
                     /locus_tag="PKU75_12180"
     CDS             complement(6441..7421)
                     /locus_tag="PKU75_12180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HPF81684.1"
                     /translation="MRAWRIAKAGEAADVYQLADIAPPVPGKGQVRVRVLGAAVNYPD
                     AMMARGHYQVQPPYPYTPGVELCGEVLELGTDTIGISVGDRIVGTADLPAGAFAEEAL
                     MNAALSQQAPGQLDDAEAAALHIAYQTGWFGLHRRAHLQRGDTLLVHAAAGGVGSAAV
                     QLGKAAGATVIGVVGGPEKASIATELGCDLVLDRNTDDLVAEIKKATNGKGVDVAYDP
                     VGGAAYDVCAKTIAFEGRILVVGFASGTVPSPGLNHALIKNYSIVGLHWGLYNAYDPA
                     AITAAATEINALVAGGIRPLISKRVSLEDAPDAVTAVGEGTTTGRVVVIP"
     gene            complement(7462..8262)
                     /locus_tag="PKU75_12185"
     CDS             complement(7462..8262)
                     /locus_tag="PKU75_12185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF81685.1"
                     /translation="MPSAPLPSPLRVVVTGAAGGIGSALAAKLAAEGAEVVVSDLPGP
                     GLEEVAVRTGGYAVPADMATADGVSALVTAAREHLGGIDAYFANAGIIGPLGIGDDDT
                     DWQRVIDVNVLAHVRAARLLLPEWLERRAGRFVVTASAAGLLNMIGAAGYGVTKHAAV
                     AFAEWLSITHGDDGVVVQALCPQGVDTTMVHTSGPVVPLLRDGAVSPEYVADLVWDAL
                     QDNRFLILPHPEVADFYRARAQDTDRWLAGMRRLNAQVFPRHTASPTA"
     gene            complement(8300..8794)
                     /locus_tag="PKU75_12190"
     CDS             complement(8300..8794)
                     /locus_tag="PKU75_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HPF81686.1"
                     /translation="MADASMDIVSKFDKQEVANAVNTAAKEIANRYDFKGVGASVELV
                     GEVVKMQANSEERCKAVLDVLQTWLIKRGVSLKHLDVPEAGPRLSGKEYKLDVPLKEG
                     ISQENAKKINKLIRDEGPKSVKTQVQGDAIRATSKSRDDLQAVQRLLKESDLDIAVSF
                     TNYK"
     gene            8863..8943
                     /locus_tag="PKU75_12195"
     tRNA            8863..8943
                     /locus_tag="PKU75_12195"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8897..8899,aa:Tyr,seq:gta)
BASE COUNT         1186 a         3287 c         2976 g         1496 t
ORIGIN      
        1 catcgccgcc ctggcccgcg gcgcccgcgt cgacgaaccc tggctgcccg acctgggcgt
       61 ctccctcacc ttcggcgccg accagatcag cgggccactc ctcatactga cggcggcgat
      121 cagcgtgctc gtcgtccttc acggacagcg ccacctgccg cccggcggcc cgcccgctct
      181 ctatttcggg ctcctgctga tcgtcgagac cggggcgctc gcgaccttct ggaccggcga
      241 cctcgtcgtc ttcttcgtcg ctttcgagat cgtgctgatc ccgatgtggg tgctcatcca
      301 ccgcttcggc gacaaccacg acccggccgc ccggcgcgag gcggccctgc ggttcgtgct
      361 cttcaccgcc ctcggctcga gcctgatgct cgccgggatc ctgggactgc tcgcccaggc
      421 cgggtcgagc agcatggcaa ccgtcgccac cgtcaccatc cccagcggca cctccctcgg
      481 gctcgccctg ctgctcgtga tcggactcgg ggtcaaggtt ccgctcttcc cgttgcactc
      541 gtggctgccg cccgcgcaca ccatcgcgcc caccggcggg tctgtgctgc ttgccgcgat
      601 cctgctcaaa atgggcacct acggcctcat ccggctcccc gtggccctga cccccgccgg
      661 attcgcgcgg ttggcgcccg tgctcgcggc gttcggtgtc gccggcattg tgtggggagg
      721 cctggtgtgc ctcatcgagc gggatctgaa gcggctgatc gcctattcgt cggtggcgca
      781 catgggtttc gtcgccctcg cgctcggcac caacagcgcc ctcggcctgc aggctgcgct
      841 cctggccaat atcgcccacg gcgtgatcgc cgccttgctc ttcttcgtcg tcggcgggct
      901 caaagagcgc tggggcagtg tcgatctcgc cgtcgtgcgg cccgcgctcc gggagacgtg
      961 gccaggtctc ggcttcgccc tgatcaccgg gctggccggc agcctcgcgc tgcccgggct
     1021 cgcgagcttc tggggtgagt tcctcgccct gaccgccgcc tggaatccgg cctacggcac
     1081 ggccgcccca tatgccgtct gggggccggc ggaacccggg ctcgtccagc ccgggtccgg
     1141 gtgggacgcg ggctgggtcg cctgggtccc accgaccggt ttcctgcgca ccctgacgat
     1201 cgtcgccgcc ttcggggcgg tgctggccgc ggcatacagc ctgcgggtgg cgcgcctggt
     1261 gtgggcgggg gacgcggcgc cgcgtgaccg cgcgggcgac ctgacctggg cggagtgggt
     1321 cgtcgccacc gtcctcatgc tcgcgatcgt caccctcggg ctggtgccgt acgtcgtcta
     1381 tccgggggtg ggctcgtgac cacctctctg gtgtcgctgg actggctcgt cctcgcccct
     1441 gtcctcgcac cggcgttcgg ggtcctgctg gtgctgctcg ccgacgcttt ggcgccgcgg
     1501 gccaggggtc tgcacctgcc gatcgctctc gtggccgtgg gcgccggtct cggcgcgttg
     1561 ccgtatgccg ccacgtccgc cgtacccgcc cgctcgctct gccgccccgg gccggaggcg
     1621 cgctgtttct acgagacggc gccgtggggg ttggccctgc agttcctcgt gctggccggt
     1681 gcgctgctcg tgctgctgat gctcgggccg ggcacgacgc acctcgatcg cacccagcgg
     1741 ggcggcccgg ccgtcaccgt cgccctcgtc ctggcggcga cgaccggtgc ggccatggtc
     1801 cccgccaccc gggacctgcc gacctggctg atcgccatcg aattggcaac cctcccggcc
     1861 atcgccctcg tcgcgctgtc gcgtggccgg ggcgccgagt ccggtgccct gtcgctgctg
     1921 atggtctcgc tcttctcctt cgccctcctc gcggtcggcg tcggcctgtg gacgctcgcc
     1981 accggtgatg cgaccttcgg ggtcgacacc ctgcgcgtcg cgttcgccga cccggaccgg
     2041 caccgggtgc tcctcctcgc gatcctcttc ctcatcgccg ggctcgggtt caagctctcg
     2101 gcggtgccct tccacctgtg gaccccgcag gccttcgtcg ttgcggacga gccaatctcg
     2161 gcgtttctcg cgaccgtgtc caaggcagcg gcactcgggg cggcgatcgt catgctcagc
     2221 cctctggctg cgctcaacgc gtccggcgat tccggggcaa catccgtccg tctcgtcatc
     2281 ggcgtgctcg cgatcgcctc gatgaccctc ggcaacctca tcgccctgcg cgcgcaggac
     2341 cccctgcgac tgctggcctg gtccacgatc gcgcaagccg gctgggtcct cgcgccgctg
     2401 gccatgctca ccccgactgc tgccagcgcc tccgtcgcct acctgctcgc gtatgccgcc
     2461 ggcaccctcg ccgtcttcgc cgtcgccgcg gccgtccaca gcggcaccga ggggtacggc
     2521 gagcggtcgc tcaccgcata cgccgggctc gggcgaggca acccggtgct cgcggccatc
     2581 ctcgcgctcg gcctgctcac cctggccggc ctgcccccgg cggtcctcgg cgttgtcgcc
     2641 aaagtcgttg cgctgcaacc ggtcgtcggg gccgggcagt ggtggctggt ggctgcggcg
     2701 atcctcaata tcgtcctcgg catcgcggtc tatctgcggt ggctgctcat cgtcgtgcgg
     2761 cccgccgggg acgcggtgcc gcagcgtctg cggctcgacc ctggcctggc cgtcgcgctc
     2821 gcgctgttgg gggccggcct gatcgtcctg agcttcgccc ccggtctggt catcggcctc
     2881 acccgctgag tcgcgccccc cacccgaagt cgaaagagca cgacacgccg taatggccat
     2941 gcgattacgg cgtgtcgtgc actttcgacg ggggtgaggc aatcgcaact tcgacgcacc
     3001 gcaacgggta tcgtccgggg aacgaaatcc ccccgagatc gcgttggacg gcatatgcac
     3061 aaccacttca acggtctgaa gaccgctgga ctcttcggct tgatctggct cctgctcctc
     3121 ggcctggggg ccgtgatcgg cagcggtcgc tacatctggc tgttcgccct catcggggtg
     3181 gcgacgacgt tctacgccta ctggaactcc gacaagatcg cgatccgggc gatgaaggcc
     3241 tacccggtca gcgaggcgca ggcgccggag atgtatcgca tcgtgcgcga gctgtccacc
     3301 aaggccggca agccgatgcc gcagctgttc atctccccga cggccgcccc caacgccttc
     3361 gccaccgggc gcaaccccag ccacgccgcc gtctgctgca ccgaggggat cctccaactc
     3421 ctcgacgaac gcgagttgcg cggggtgctc gggcacgagc tgatgcacgt ctacaaccgc
     3481 gacatcctca ccggctccgt cgctgcggcg atcgccggca tcatcacctc ggtcgcgcag
     3541 atggcgatgt tcttcggcgg cgggtccgac gaggaccggc ccaacccgct ggcgctgctc
     3601 ggcatggcgc tgctcgcacc gttcgcggcg atgctcatcc agatggccat cactcgcacc
     3661 cgcgagtacg acgcggacga ggacggtgcc aagctcaccg gcgacccgct cgccttggcg
     3721 tccgcgttgc gcaagctcga gaccggcacc cgccgcgctc cgctcgcgcc gacgccgcag
     3781 gtcgtcaatg cctcgcacat gatgatcgcc aaccccttcc gggcccagga cgtctcccgc
     3841 ttcttcgcca cccacccgcc gatggccgac cggatcgctc gtctggaggc gatggccggc
     3901 ggtatcgacc ggcgccgctg acggcatacc tcccagcggc cccacgaggc ctcgcgacag
     3961 ggcaggatgg cagttgatgt cccctctgac gcgcaccgaa gcccagcagc gagccacgac
     4021 catccgcgtc agccggatgg tcgtggcgct cgacctcgac caggggccgg agaccttcgg
     4081 ctcgcgagcg gagatccatt tcgaggccac cgcacccgag accttcgtcg acctgcgccc
     4141 ggtcggggtg gcccgcctcg aactcaacgg ccaggacctc gatccggcgg cgctcgtcga
     4201 cggccggttg gcgctgaccg ggctggagcc gagcaatgtg ctgatcgtcg aggcgacgat
     4261 ggcctacagc cgcgacgggc aggggctgca ccgcagcgtc gaccccgccg acgaggaggc
     4321 gtacgtctat ggccatctct tcctcgacgc cgccccgacc gtgttcgcgt gtttcgacca
     4381 gccggacatc aaggcgccgt atgccgtgag cgtgcgcgcc cccgccgact ggcgggtgct
     4441 cggcaacggg gccgcgaccc gcgtggccga gggctgctgg gagctggccg agacgccgcc
     4501 gctcgcgacc tacttcgtca ccgtctgcgc gggtccgtgg gcgagcgtcg agcgcgagca
     4561 cgacggcata cggctcggcg tgcactcccg aaggtcgctc tcggaagccc ttgagcggca
     4621 ggcagattcg ctcctgaacg cgaccgctga cttcttcgac tactaccacg agctgttcgg
     4681 gattcgctac ccgttcggcg attatcacca ggtcttctgc ccggagttca acgccggcgc
     4741 gatggagaac cccggctgcg tcacgatccg cgacacctat ctcttccgcg gcagcgccac
     4801 ccacgacgaa ctgctcaccc gcaccaacac gatcgcgcac gaaatggcgc acatgtggtt
     4861 cggcgacctg gtgacgatga cgtggtggga cgacctgtgg ctcaacgagt cgttcgccga
     4921 atacctcgcc caccgcgcgt gcgtggcggc cagtgagtat gccgacgcgt gggtcgactc
     4981 gacgatggcc cgcaagttgt ggggtcactc cgccgagcgt tcgccgtcca gccaccccgt
     5041 cgccgggtcg cccgcccagg acgcacagac ggcgctgaac aacttcgacg gcatctccta
     5101 cgccaagggc agcgccgtgc tgcgtcaact catcgcgcac atcggggacg acgccttcgt
     5161 cgccggactc cgggatcact tgcagcgcaa cagtttcggc aatgcggacc tggccggctt
     5221 tctcgcggcc atcgagcggg agtcaggatc ggacctcagc ggctggagtc acgcgtggct
     5281 cgagacggcc gggatcgata ctctgcacgt ccaccaggac accgggcggg tgacccgcag
     5341 cacgccggac cggttcgcga aggtgagccg gccgcatacc ttcgacatcg ccggattcac
     5401 cagcggcaca gcggttttcc gggtgacggg caccatcgac cgggccacgc gcaagtggcc
     5461 ggagctgctc gccgccgacc gcgccgaggt cgtcataccc aacgcgtccg acctgacgtg
     5521 ggccgtcagc gggctggagc cggaaagcct cgccaacctg cccgacgccc tggcgagcat
     5581 ccccgacgcc caatcccgcg cggtcgcctg gctggccctg atcgacggga tgcacgaggc
     5641 ccgcatcgac ccgcggctgc tgatgcgcac gctcaccatg gcctggccgc tggagagcaa
     5701 cggctcgatc ctcggccgcg tcggcaatca cgtcatcacc cgggccatcc cggtgttcct
     5761 gccgccgacc gaacagcctg gcggcgagga ggcgatggcc accgctgcac gccggttgta
     5821 tgacggtgcg accagtgccg accaccgcct catcgccgcc cgcctgcttg ccgccaccag
     5881 cagcgacgag acgctcttgc ggtcctggct cgacgggatc gacctgccgc cggaactcgt
     5941 cgacgactcc gacttccggt ggttcgtggt ccgggccatg tcacgccgcg ggctcctgga
     6001 cgagcagggg atcgagtcct tccggcggcg ggacgagagt cttgccggcc agcaggcagc
     6061 gcttgccgcc cgggccctgc gaccgacggc cgaggcgaag gagtgggcct ggagcgaact
     6121 gaccggcaac ccggcgcgct cgaactacga actcaacgcc ctcgccggcg ccttctggca
     6181 gacgaccgac cgcgacctcg tatctcctta tgtggagcgc tatttcaccg acgttcccgg
     6241 cctggccggt cgcctcggag aggacgctct cgggaccgtc gcggccctgg cctacccgct
     6301 gccgctcgtc gcatcccgca ccctcgacct gagccgggcg gcgctggcga gggacaccct
     6361 cagccccgcc gtccgccgtt ccatcgtcga cgcccaagcc aagcttgagg agggcctggc
     6421 ctcccgggcc gcgttccccg ctacgggatg actacgactc gtccggtggt cgtgccctca
     6481 ccgacggccg tgacggcgtc gggtgcatcc tcgagagaca cgcgcttgct gatgaggggt
     6541 cgtatgccgc ccgccaccag ggcgttgatc tccgtcgccg ccgccgtgat ggctgcgggg
     6601 tcgtaggcgt tgtagagacc ccagtgcagg cccacgatcg aatagttctt gatgagggcg
     6661 tggttcaggc cggggctggg gaccgtgccg ctggcgaacc cgacgacgag gatgcggccc
     6721 tcgaaggcga tcgtcttggc gcacacgtcg tatgccgcac cgcccaccgg gtcgtaggcg
     6781 acgtccacgc ccttgccatt ggtcgccttc ttgatctcgg cgacgaggtc gtccgtgttg
     6841 cggtccagga cgagatcgca tccgagttcc gttgcgatgg atgccttttc gggcccgccg
     6901 acgacgccga tgaccgtggc gccggcggcc ttgccgagct ggacggccgc actgccgaca
     6961 cctccggctg cggcgtgcac gagcagggtg tcaccgcgct gcaggtgcgc gcgccggtgc
     7021 aggccgaacc acccggtctg gtaggcgatg tgcagcgccg cggcctcggc gtcgtcgagt
     7081 tgaccgggag cctgctggct cagggccgcg ttcatcaacg cctcctcggc gaaggccccc
     7141 gcgggcaggt cggccgtgcc gacgatccgg tcgccgacac tgatgccgat ggtgtcggtg
     7201 cccaactcca gcacctcgcc gcacagctcg acgccggggg tgtagggata gggcggctgc
     7261 acctgatagt gcccgcgcgc catcatcgcg tcgggatagt tcacggccgc gccgagcacc
     7321 ctgacccgca cctggccctt gccggggacc ggcggtgcga tgtccgccag ctgatagacg
     7381 tcggcggcct cgccggcctt cgcgattcgc cacgcgcgca tagttcttcg gtgctccttg
     7441 ggttggttcg gttcgtcagt ctcaggcggt gggtgaggcg gtatgccgcg ggaagacctg
     7501 cgcgttcagc cgccgcatcc cggcgagcca gcggtcggtg tcctgcgccc gcgcgcgata
     7561 gaagtcggcg acctcggggt gggggaggat gaggaacctg ttgtcctgca aggcatccca
     7621 caccaagtcg gcgacgtatt ccgggctgac ggcaccatcg cgcagcaacg ggacgactgg
     7681 gccggaggtg tgcaccatcg tcgtatcgac gccttgcggg cagagggcct ggacgacgac
     7741 cccgtcgtcg ccgtgggtga tgctcagcca ctcggcgaag gcgacggcgg cgtgcttggt
     7801 gacgccgtag ccggccgcac cgatcatgtt cagcaagccg gcggccgagg ccgtgacgac
     7861 gaaccggccg gcccgacgct ccagccattc gggcagcagc agccgcgcgg cccgcacgtg
     7921 cgccaggacg ttgacgtcga tgacccgctg ccagtccgtg tcgtcgtcgc cgatgccgag
     7981 cggcccgatg atcccggcgt tcgcgaaata ggcgtcgatc ccgcccaggt gctcacgcgc
     8041 ggcggtgacc aacgccgaca ccccgtccgc ggtcgccatg tcggcgggca cggcataccc
     8101 acccgttcgc accgcgacct cctccagacc cgggcccggc agatcactga cgaccacctc
     8161 ggcgccctcg gcggccagtt tggccgccag cgccgacccg atgcccccgg ctgctccggt
     8221 cacgacgacg cgcagaggcg aggggagggg ggcggacggc ataacgctca atctaggcga
     8281 gccgacgggc ggtggtggcc tacttgtagt tggtgaagct cacagcgatg tcgaggtcgg
     8341 actccttgag gaggcgctgg acggcctgca ggtcgtcgcg gctcttgctc gtcgcgcgga
     8401 tagcgtcgcc ctggacctgg gtcttgaccg acttgggccc ctcgtcgcgg atcagcttgt
     8461 tgatcttctt ggcgttctcc tggctgatgc cctccttgag gggcacgtcg agcttgtact
     8521 ccttgcccga cagccgcggg ccggcctcgg ggacatcgag gtgcttcagc gacaccccgc
     8581 gcttgatcag ccaggtctgg aggacgtcga ggacggcctt gcaccgctcc tcggagttgg
     8641 cctgcatctt cacgacctcg ccgacgagtt cgacggaggc ccccacgccc ttgaagtcgt
     8701 aacggttggc gatctccttg gccgccgtgt tgacggcgtt ggcgacctcc tgcttgtcga
     8761 acttgctgac gatgtccatg ctggcgtcgg ccacggcata cctctcatcg gatctgatta
     8821 gggatcgagc gatctggctt gctatccttt cacgcgcacc acggcaggtt gtccgagcgg
     8881 ccaatggaag cggactgtaa atccgtcgcg aaagctacga aggttcgaat ccttcacctg
     8941 ccacc
//
LOCUS       DAOPJG010000143         8897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8306_length_8897_cov_6.197354, whole genome shotgun sequence.
ACCESSION   DAOPJG010000143 DAOPJG010000000
VERSION     DAOPJG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8306_length_8897_cov_6.197354"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..2212
                     /locus_tag="PKU75_12200"
     CDS             206..2212
                     /locus_tag="PKU75_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPF81687.1"
                     /translation="MDEPPEQVPELHPERTTHPFADLTQEPGAAPVKRARTKRKLNVP
                     YARAGDPVPVVTPKGDLRRHAAIDYDLNPQSTHWVVLALKSATPGLHFLTHQYRREWL
                     RPDVIAGLAVAAYLIPQVMAYTAIVRVPPVYGLYTALVALVVYALMGSSRILSVGPES
                     TIALLAGTAIAGLAPDPGAIVALSAAMSLLVGGWLLLARLFRLGVMADLLSQPLLVGY
                     LAGAAVLMVVGQLGKLTGTSVDGESIIAQARSFLDVVGATHLTTLYVALGTAAFIFVL
                     HWIKPSWPGALLAVAGATIASALLDLEAKGVAVVGSVPNGLPLPQWPAVTMDQLQALL
                     LPALSVAVLAYSDNMLGARAFPSPEIPGERPSERAVDPQVELVALGGVHVAVGAFGGF
                     PVSSSGSRTALALSAGAKSQVYSLVAAGCVLAVLLVAGPLMAKLPQAALGAVVLYAAT
                     KLVKGGEFRRMWRFRKREVLLALVTLVGTIVLGLIVGIGVAIAISLLEMAQRLARPRD
                     AVLGRVPDLPGMHDTADYPSAETLPGLIIYRYEAPLFFANVGDLRRRVQRVVDKEFAG
                     YPDAPPRWFLFNVEATTEVDVTACDGLRDLYADLAAQGVRLGLVRLRQDLYIPLRKAG
                     VMKLIGDDMVFPTLPVAEEAYVAWARAHPAPPPPTLAPVTEDEE"
     gene            2359..2432
                     /locus_tag="PKU75_12205"
     tRNA            2359..2432
                     /locus_tag="PKU75_12205"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2393..2395,aa:Phe,seq:gaa)
     gene            complement(2660..3427)
                     /locus_tag="PKU75_12210"
     CDS             complement(2660..3427)
                     /locus_tag="PKU75_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPF81688.1"
                     /translation="MGPSASPGADELRLPSAHVHAIARDPGSGNVVVAAHDGLYVYDG
                     ATPKRVGPVIDLMGFTVAGPGHYYASGHPGTATDLPQPVGLIESRDGGNTWTVLSRGG
                     ASDFHALTTAGSTVVGFDGVLRSTADGRTWRDGNLDAQPRTLAGSRDGATVLATTPEG
                     LTHSTDAGLSWRWSEQAPLLLMVAFGDGTTVAGLTPDGQVHTSTDAGQTWRATELLAP
                     DAQALHASGEGEQLEILVANDRTILASTGGQPFQSMR"
     gene            complement(3586..4176)
                     /locus_tag="PKU75_12215"
     CDS             complement(3586..4176)
                     /locus_tag="PKU75_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HPF81689.1"
                     /translation="MRLTSRLAIPAASAVLLLALSACGSGDDSHTTMSGAGTTSSTMA
                     EGTTTTATSGDVEFAQMMIPHHEQAVEMADVALANDTASPEVKALAAQIKAAQDPEIQ
                     TMKGWLAQWGASESAGQMDHGGMMSDEDMSSLMGISGPEFNQMWLTMMIEHHEGAVEM
                     AQDVLDTTSNPEVEQLATAVVQGQEKEITTMKGMLG"
     gene            complement(4240..6693)
                     /locus_tag="PKU75_12220"
     CDS             complement(4240..6693)
                     /locus_tag="PKU75_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPF81690.1"
                     /translation="MSQTTVLHVGGLHWATSEHAIESILSRREGVEAVEANAANQSAT
                     VTYDPDRTSVAELAEWVRECGYHCAGRSVPMHVCEPMDEDPVPEAARAHAHHADQARH
                     PAAHVDATHAGHTAVTTATTAAEHSPHEMMGHGGHGGMSMDDMVRDMRNRFVVAALMT
                     VGIALWSPMGRDMLGFTAPAPFGLRDDVFALLLSLPVVFYSAWIFFDGAYRALRNRTL
                     DMMVLVAVAVAAGWLYSVGITLTGGGEVFYEAAAMLTAFVLLGHWFEMRARGGANEAV
                     RALMDLAPPMATVVRDGEPVEVPTADVLVGDLVLIRPGGKVPVDGTVEDGTSEVDESM
                     VTGESLPVAKTPGSTVIGASINTTGTLRVRATKVGSDTALAQIVALVQQAQNSKAPGQ
                     RLADRAAFWLVLVALFGGLLTFAVWLLSGASVETALLFAITVVVITCPDALGLATPTA
                     IMVGTGLGAQRGVLVKNATALEASARIDTVVMDKTGTLTKGEPEVTDVIVDGIDETEL
                     LALVAALENESEHPLAAAIVGHAAQREVVPERPTEFRNVPGHGAVALVNGRRVVVGNR
                     RLMDDEGIDVSAMTARRDKLAASGRTAVLVAVDGRAVAVIGLADAVRDTSAAAVAVLH
                     DMGVQVVMLSGDNEATARRIAGQLGIDTVLAEVLPGDKAAEVARLQQAGRKVAMVGDG
                     VNDAPALAQADLGIAIGAGTDVAIETADVVLMRSDPLDVPTALRIGRGTLRKMRENLS
                     WAVGYNAIALPIAAGVFASWGLVLRPEMAALSMSGSSVIVAVNALMLKRLRLPRPEAE
                     ATAVSDGQAPSSNLHETTG"
     gene            complement(6993..7271)
                     /locus_tag="PKU75_12225"
     CDS             complement(6993..7271)
                     /locus_tag="PKU75_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81691.1"
                     /translation="MNNHAIDPAHGDVHADLPATGSSLGAQPQPGPEKHERPWRHHLL
                     MMVMCLPMLVIVGALVITGVAGAGAIGYAVLCTAMMAAMMMLMRGDRH"
     gene            complement(7497..7706)
                     /locus_tag="PKU75_12230"
     CDS             complement(7497..7706)
                     /locus_tag="PKU75_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81692.1"
                     /translation="MTRFALRFAATAASVTLGLVMASSASAAPADAAVYGQHVRDCAQ
                     SMGFDQNHNPGMHQGISMWDPAHTC"
     gene            complement(7857..8099)
                     /locus_tag="PKU75_12235"
     CDS             complement(7857..8099)
                     /locus_tag="PKU75_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HPF81693.1"
                     /translation="MMWGNGMGWGGWLLMTLTTVAFWAVVVFGIVALFRGSGGPGPRD
                     GSRDRDARQILDERFARGEIDAEEYRSRQSILSTHG"
     gene            8250..>8897
                     /locus_tag="PKU75_12240"
     CDS             8250..>8897
                     /locus_tag="PKU75_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF81694.1"
                     /translation="MPATDRDPRSAPGDAEPLARVATRALVVEDEVEMAQVIGSYLER
                     EGFEVHLSHDGVDAVARAREVDPDIVVLDLGLPGQDGLEVCRQLRTFSDAYVVMLTAR
                     TDEVDTLIGLSVGADDYLTKPFSPRELVARLRAMLRRPRPIGTPAPDTAGFRVFGPLS
                     IDPTGRDVWLHDEPIALTRTEFDVLAVLSERPRLAYTRRQLIDAVWGQTWVGDEHL"
BASE COUNT         1246 a         3060 c         3124 g         1467 t
ORIGIN      
        1 gtagcgcagt tggttagcgc gccgccctgt cacggcggag gtcgcgggtt cgagccccgt
       61 cggggtcgcg tcagatgcga cacgcgggcg gttcctcctc gggagccgcc cgcttcgcct
      121 ttcccccgcc accgcctctc ccccgccacc cccgggcgcg cgtacgcttc aggtgaacac
      181 caggtgaacg tcaggggaga cgcagatgga cgagccgccc gagcaggtgc cggagcttca
      241 tccagaacgc accacgcacc ccttcgccga tctcacccag gagcccggtg cggccccggt
      301 caagcgggcg cgaacgaagc gcaagctgaa cgtgccgtat gcccgcgcag gtgaccccgt
      361 ccccgtggtc acccccaagg gcgacctgcg gcgccacgcg gccatcgact acgacctcaa
      421 cccccagtcc acgcactggg tggtgctggc cctcaaatcc gcaacaccgg gcctgcactt
      481 cctcacccac caataccggc gcgagtggct gcgaccggac gtgatcgcgg ggctggccgt
      541 cgcggcatac ctgatccctc aggtgatggc ctataccgcc atcgtgcgcg tgccgcccgt
      601 ctacggcctt tacaccgccc tcgtcgccct cgtcgtctac gccctgatgg gcagctcacg
      661 gatcctgtcg gtggggcccg agtcgacgat cgcactgctc gccgggaccg ccatcgcggg
      721 cctggccccc gacccgggcg ccatcgtcgc gctcagcgcc gcgatgagcc tgctggtcgg
      781 cgggtggctg ttgctggcgc gtctgttccg cctcggggtg atggcggacc tgctgtccca
      841 gccactgctg gtgggctatc tcgccggggc ggcggtcctg atggtcgtcg gccaattggg
      901 caagctcacc ggcaccagcg tcgatggcga gtccatcatc gcccaggccc gctcctttct
      961 cgacgtggtc ggcgcgaccc acctgacgac tctctatgtc gcactgggga cggccgcctt
     1021 catcttcgtg ctgcattgga tcaagcccag ctggcccggt gccctgctgg ccgtggccgg
     1081 cgccaccatt gcctcggcac tgctggacct ggaggccaag ggcgttgcgg tcgtcggttc
     1141 ggtcccgaac ggcctgccgc tgccgcagtg gccggcggtc accatggacc aactgcaggc
     1201 cttgctcctg cctgccctca gcgtcgcggt gctcgcctac agcgacaaca tgctgggcgc
     1261 ccgcgccttc ccctcccccg agatccccgg cgaacgcccg agcgagcgcg ccgtcgaccc
     1321 gcaggtcgaa ctcgtggcgc tcgggggcgt ccacgtcgcc gtcggcgcct tcggtggttt
     1381 cccggtctcc tcctccggct cgcgcacagc gctcgccctc tcggccggcg ccaagagtca
     1441 ggtctattcg ctcgtcgcgg ccggctgtgt gctcgccgtc ctgctcgtcg ccgggccgct
     1501 catggcgaag ctgccgcagg ccgccctcgg cgccgtggtg ctgtatgccg ccaccaagct
     1561 cgtcaagggc ggcgaattcc gccggatgtg gcgtttccgc aagcgcgagg tgctgctcgc
     1621 gctggtcact ctcgtcggca cgatcgtgct cggcctgatc gtcggcatcg gggtcgccat
     1681 cgcgatcagc ctcctggaga tggcccagcg gctggcccgc ccgcgcgacg ccgtgctcgg
     1741 ccgcgtgccg gacctgcccg gtatgcacga caccgccgac tatcccagcg ccgagacgct
     1801 gcccgggctg atcatctacc gctacgaggc accgctcttc ttcgccaatg tcggggacct
     1861 gcgccgccgg gtccagcggg tggtggacaa ggagttcgcc ggctatccgg acgcgccacc
     1921 gcgctggttc ctcttcaacg tcgaggcgac gaccgaggtc gacgtcaccg cgtgcgacgg
     1981 gctgcgggat ctgtatgccg atctcgcagc tcaaggggtc cggctcggcc tggtgcgcct
     2041 gcgccaggac ctctacatcc cgctgcgtaa ggccggcgtg atgaagctga tcggcgacga
     2101 catggtcttc cccaccctcc cggtggccga ggaggcgtat gtcgcgtggg cgcgcgccca
     2161 ccccgccccg cctcctccta cgctcgcccc cgtgaccgag gacgaggagt gagcgcggct
     2221 agccgcgggt agggctgccg atcggcaccg catacggtgc caaccagcag acaccactgc
     2281 cacgcggcac atgaggacgg tgaatttcat cgctcgtcag gcggcgggta tgctggctcg
     2341 gccggagcga tccggctcgg ctaggtagct cagttggtac gagcatccga ctgaaaatcg
     2401 gaaggtcgac ggttcgaccc cgtccctagc cacacaaaac cgcaggtcac aggcttgcta
     2461 gggaccagcg acattcagtc gttgctaacg cttgctaaca caacggtggg acgtaacgct
     2521 ttcagccgtc ccggcgattc tggtgaatgc accccggctc ggagccgcag gagggcgcac
     2581 cggagccctg gccctggcct ttgggttcgc ttgacagggg cggagccgtg gggttgtcgc
     2641 acccagctca ttcgctgggt cagcgcatgg actggaacgg ctggccgccg gtggaggcaa
     2701 ggattgtccg gtcgttcgcg acgaggatct ccagctgctc tccttcgcct gatgcgtgca
     2761 gtgcttgcgc gtcgggtgcg aggagttcgg tagcgcgcca ggtctgaccg gcgtcggtgc
     2821 tggtgtggac ctgtccatcg ggtgtgagac cggcgacggt ggtcccgtca ccgaaggcca
     2881 ccatcagtag aaggggcgcc tgctcgctcc agcgccagct gagcccggca tctgtggagt
     2941 gcgtcaggcc ctctggcgtg gtcgcgagaa ccgtggcccc gtcgcgtgag ccggccagcg
     3001 tccggggttg ggcgtcgagg ttcccgtcgc gccaggtccg gccgtcggca gtgctgcgca
     3061 ggacgccgtc gaatccgacg acggtgctgc cggcggtggt cagtgcatgg aagtccgaag
     3121 cgcctccgcg ggacaggacg gtccaggtgt tcccgccatc gcgtgactcg atgagcccga
     3181 ccggttgcgg gaggtcggtc gcggtcccgg ggtgcccgga ggcgtagtag tggcctggtc
     3241 cggccacggt gaaccccatg aggtcgatga ctggcccgac ccgcttcggc gtcgccccgt
     3301 catagacgta cagcccgtcg tgggcggcga caaccacgtt gccgctgccg gggtcacggg
     3361 cgatggcatg gacgtgcgcc gagggaagcc tcagctcgtc ggcgcccggg ctggctgacg
     3421 ggcccacggg ttctggactt ggcgtgccgc cccatgcgtc gctggtggcg gccgcggggg
     3481 cgggtgccga agcaggtgag gcggagcacg ccgccacgag cccgacggag atcaccgcga
     3541 cgagcaggct gagtggtcgt gacggggaga gtcgctcacg catggtcagc cgagcatccc
     3601 cttcatggta gtgatctcct tctcctggcc ttgcacgacc gcagtcgcca gctgctccac
     3661 ctcagggttt gacgtggtgt caagcacgtc ctgagccatc tcgacggcgc cctcgtggtg
     3721 ctcgatcatc atcgtgagcc acatctggtt gaactcgggg ccggagatgc ccatcagtga
     3781 gctcatgtcc tcgtcgctca tcatcccgcc gtgatccatc tggcctgcgc tctccgaggc
     3841 cccccactgg gcgagccacc ccttcatggt ctggatctcg ggatcctgcg cggccttgat
     3901 ctgggcggcc agggctttga cctcgggcga tgcggtgtcg ttcgccaggg caacgtcggc
     3961 catctccacg gcctgttcgt ggtgcgggat catcatctgc gcgaactcca cgtctcccga
     4021 ggtagcggtg gtcgtcgtgc cctcagccat ggtcgaggag gtggtgcctg caccggacat
     4081 cgtggtgtgg gagtcgtcgc cggagccgca ggcgctcaac gccaggagga ggacggcgga
     4141 cgcggcgggg atggcaagtc gagaggtcag acgcacgaga ggtctcctgg aactggggct
     4201 ggagcggaaa ggacgatcct gtccggcgga ccgtagaggt cagccggtgg tttcatgaag
     4261 gttcgatgaa ggtgcctgcc cgtcgctgac agctgtcgcc tctgcctcgg gtcggggcag
     4321 ccggagccgt ttgagcatga gggcgttgac ggcgacgatg acgctggacc cggacatgga
     4381 cagggcggcc atctcggggc gcaggaccag accccagctg gcgaagaccc cggccgcgat
     4441 gggtagggcg atcgcgttgt agcccaccgc ccaggacagg ttctcccgca tcttgcgcag
     4501 cgtgcccctg ccgatgcgca gcgccgtggg tacgtccagg gggtcggagc gcatgaggac
     4561 tacgtcagct gtctcgatgg ctacgtcggt tccggcgccg atggcgatgc cgaggtctgc
     4621 ctgcgccagc gccggggcgt cgttgacgcc atccccgacc atggcgacct tgcggccggc
     4681 ctgctggagc cgggccacct cggcggcctt gtcaccgggg agcacctcag ccaggacggt
     4741 gtcgatgccg agttggccgg cgatgcgtcg cgcggtggcc tcgttgtcgc cgctgagcat
     4801 gacgacctgg actcccatgt cgtgcaggac cgcgacggcg gccgcggagg tgtcgcgcac
     4861 ggcatccgcc agaccgatga ctgcgacggc ccggccgtcg acggcgacca ggaccgcggt
     4921 gcgtccgctg gcggccagct tgtcgcgacg tgcggtcatg gccgacacgt cgatgccctc
     4981 gtcgtccatg agcctgcggt tgcccacgac cacccgccgt ccgttcacga gggcgacggc
     5041 gccgtggccg ggtacgttgc ggaactcggt ggggcgctcg gggacgactt cgcgctgcgc
     5101 ggcatggccg acgatggctg cggccagggg gtgttcggac tcgttctcga gggcggcgac
     5161 cagggcgagc agctcggtct cgtcgatgcc gtcgacgatg acgtcggtga cctccggctc
     5221 gcccttggtc agggtgccgg tcttgtccat gacgacggtg tcgatgcggg ccgaggcctc
     5281 cagcgcggtg gcgttcttga cgagcacgcc gcgttgggcg cccaggccgg tgccgaccat
     5341 gatcgcggtc ggggtggcca gcccgagggc gtcggggcag gtgatgacca cgacggtgat
     5401 ggcgaacagc agagcagtct cgacgctggc accggagagc agccacaccg cgaaggtcag
     5461 caggccgccg aagagcgcga ccaggaccag ccagaacgcg gcccggtcgg ccagtcgctg
     5521 cccgggggcc ttggagttct gcgcctgctg caccagggcg acgatctgcg ccagcgcggt
     5581 gtccgagccg accttggtcg cccgcacccg cagcgtcccg gtcgtgttga tcgaggcgcc
     5641 gatgaccgtc gagcccggcg tcttggcgac gggcaggctc tccccggtca ccatcgactc
     5701 gtccacctct gacgtgccgt cctcgacggt gccgtccacc gggaccttcc cgccgggacg
     5761 gatgaggacc aggtcgccga cgaggacgtc agcggtgggg acctcgaccg gctcaccgtc
     5821 gcgaaccacg gtggccatcg gcggggccag gtccatgagg gcgcgcactg cctcgttcgc
     5881 gcccccgcgg gcacgcatct cgaaccagtg gcccagcagc acgaatgccg tgagcatcgc
     5941 cgcggcctcg tagaacacct cgccgccacc ggtcagagtg atgccgacgc tgtacaacca
     6001 gcccgcagcg accgcgaccg cgacgaggac catcatgtcc agggtgcggt tgcgcagagc
     6061 ccggtaggcg ccgtcgaaga agatccaggc cgagtagaac accacgggca ggctcagcag
     6121 cagcgcgaac acgtcatcgc gcagcccgaa cggtgccggg gccgtgaagc cgagcatgtc
     6181 gcggcccatg ggcgaccaca gggcgatccc caccgtcatc agtgcggcaa cgacgaaccg
     6241 attgcgcatg tcgcggacca tgtcgtccat ggacatgccg ccgtggccgc cgtggcccat
     6301 catctcgtgc ggtgagtgct cagccgcagt cgttgccgtg gtgacagccg tgtgtcccgc
     6361 gtgggtggcg tcgacgtgag cggccgggtg cctcgcctga tcggcgtggt gcgcgtgtgc
     6421 gcgagcagcc tcgggcactg ggtcctcgtc cattggctcg cacacgtgca tggggacgga
     6481 gcggccagcg cagtggtagc cgcactcgcg cacccactcg gccagttcgg ccacggaggt
     6541 tcggtctggg tcgtaggtga ccgtggcgct ctggttggcg gcgttggcct cgaccgcttc
     6601 gacgccttcg cgccgcgaca ggatggactc gatggcgtgt tcactggtgg cccagtgcag
     6661 tccgccgacg tgcagaaccg tggtctgact catggtgatc tacttcgctt tcaactggtt
     6721 cccgaggtgc ttctctcccg tccaacctga taccccctgg gggtattccc ggggaggtgt
     6781 ctccgacctc gtagcggatg gctgcagtgc tcgtgatgcg cccgccggct gtgtgagttg
     6841 ccggctgtcc cgggcacgcc gcccgcagcg cgtgacgctg caccagccgg cacgcaaggg
     6901 tgagattggc ggcgcgatca tgctcgagct gcggacgcgg ccggcaactc agcggagccc
     6961 tcggtgagtc gccctggcac cgaggggtgc gctcagtgcc gatctccccg catcagcatc
     7021 atcatggcgg ccatcatcgc ggtgcagagc acggcgtatc cgattgctcc agcgccggca
     7081 acgccggtga tgaccaaggc gccgacgatc accagcatgg gcaggcacat gaccatcatc
     7141 agcaggtggt ggcgccacgg gcgttcgtgc ttctcagggc ctggctgcgg ttgcgccccg
     7201 aggctgcttc ctgtggcggg taggtcggcg tgcacgtcgc cgtgggcagg gtcgatggca
     7261 tggttgttca tgggggacgt cctttcaggg ttcgcgggtt gtgatcgagc cgcgctacgg
     7321 gcgcgtactc cgttcgagcc ggcggcgccg gaggcgagtg acgggagcgc ggtggggctc
     7381 cggctggatg cgccgaattg tcaccgcccg cccggtgggg ccgcagcttc aggtgagctt
     7441 cgcgagctgc ccgtgagcgc gggtgagttc tcaccggcgg cctcgccacg ttggtgtcag
     7501 caggtgtggg cggggtccca catcgagatg ccttggtgca tgcccgggtt gtggttctgg
     7561 tcgaagccca tggactgggc gcagtcgcgc acgtgctggc cgtacaccgc cgcatctgcg
     7621 ggtgctgccg atgccgagga ggccattacc aggcccaggg tgacggatgc ggctgtggcg
     7681 gcgaacctca gggcgaagcg ggtcatgacg ggttcctcat tctcgtgatg gacgtgcgtg
     7741 aagggtggaa gctgcggcca gcgcgggagc gctgaccacg ggtggcccct gacgtggcag
     7801 gcgcaccgtg gtgggtagat cccggcctgt ccgaggacgt tctttcgatg cccggttcat
     7861 ccgtgagtgg acaggatgct ctgtcgggag cggtactcct cggcgtcgat ctcgccgcgg
     7921 gcgaaccgct cgtccaggat ctgccgggca tccctgtcac gactcccgtc gcgcggaccc
     7981 gggcctccgc taccccggaa gagtgccacg atcccgaaga cgaccacggc ccagaaggcc
     8041 accgtcgtca gcgtcatcag gagccatccg ccccatccca ttccgttgcc ccacatcatg
     8101 gtgctcacct cttcgcgtca ggtcgatggg gccaccctcc tcggccagcc cccgctgcgc
     8161 ggtcgcggtt ccatgaaggt tcgctgaaga tgtgcgccca gaccctgtgg gggcagtgaa
     8221 cggtgcggcg cggctgcgac gatgacggca tgcccgccac tgaccgcgac cccaggtcgg
     8281 cgcccggcga cgccgaaccc ctcgcccggg tcgccaccag ggcgctggtt gtggaggacg
     8341 aggtcgagat ggcccaggtg atcggcagct acctcgaacg tgagggcttc gaggtccacc
     8401 tcagccacga cggtgtggat gccgtggcca gagctcgcga ggtcgacccc gacatcgtcg
     8461 tgctcgacct ggggttgccc ggccaggacg gcctcgaggt ctgtcggcag ctgcggacgt
     8521 tctctgacgc gtacgtggtg atgctcacgg cccgcaccga cgaggtggac accctgatcg
     8581 gcctgtcggt cggcgcggac gactacctca ccaagccgtt cagcccgcgc gagctcgttg
     8641 cccgcctgcg ggccatgctc cgccgccccc gacccatcgg cacccccgcc ccggacacgg
     8701 caggcttcag ggtgttcggc cccttgagca tcgaccccac cgggcgggat gtctggctgc
     8761 acgacgagcc aatcgccctg acccgcaccg agttcgacgt ccttgctgtg ctgtccgagc
     8821 gcccccgact ggcctacacc aggcgccagc tcatcgacgc cgtctggggg cagacctggg
     8881 tcggcgacga gcacctc
//
LOCUS       DAOPJG010000144         8867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8339_length_8867_cov_7.054698, whole genome shotgun sequence.
ACCESSION   DAOPJG010000144 DAOPJG010000000
VERSION     DAOPJG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8339_length_8867_cov_7.054698"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..918
                     /locus_tag="PKU75_12245"
     CDS             184..918
                     /locus_tag="PKU75_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81695.1"
                     /translation="MSDSVFDQNKVVDQNYVIDQDKLPGGQPQQSTSSQAVDEAKSVA
                     ADAASSGKAVAGAAKEEVAQVAGEAKNHAQDLYHQARSEVSSQAATQQDRVAGLLRLL
                     TSELQEMGRSSQSGIASGLVSQAADQTERVAGWLESRDPSDLLEDIRRYARRNPGNFL
                     AGAALAGFLGGRITRSLASQDDDADQQYVQRQRPVTTVPAGAVTTQHIPSAVPAYGGS
                     PTPATAPNPASVRTGTTREAGGELPR"
     gene            915..1421
                     /locus_tag="PKU75_12250"
     CDS             915..1421
                     /locus_tag="PKU75_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPF81696.1"
                     /translation="MSQVGHEGSTAAGPPVREPAEIGEVRPAEQIEGAHDTYDKGEIG
                     SIGALLGDIAGDVTTLMRQEVALAKAEVRQSATDAGKGAGMLAGAGVAGHLALLFLSI
                     ALWWALGSWWDNFGVAALVVGVIWALIAAVLAARGRTELKNVTGVPRTVETVKQVPDA
                     LKGNEDPR"
     gene            1418..>2074
                     /locus_tag="PKU75_12255"
     CDS             1418..>2074
                     /locus_tag="PKU75_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HPF81697.1"
                     /translation="MSNDIPTIDSNDPEQLRRDIEQTRVNLSQNVNALGEAVEPGAVA
                     KRQVEKVQDKVVGAGRGLKEKVMGSDDDYGDSSGLTGKLSDAGDTVGDMAASARAQVE
                     NAPSAARRRTRGNPLAAGLVALGAGWLLGSLLPASEKERQLAATAKDAVEEKGQPLLE
                     EAKSVVQEAAENLKEPAQDAANALKESAQESVEKVKAEGQGAAQTVKESAQESKDAVQ
                     R"
     assembly_gap    2075..2174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2492..4960)
                     /locus_tag="PKU75_12260"
     CDS             complement(2492..4960)
                     /locus_tag="PKU75_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HPF81698.1"
                     /translation="MTEPSSWPNGASSPMTEDEARLLDAWWRAANYLSVGQIYLLDNP
                     LLREPLTRDHVKPRLLGHWGTTPGLTFLYAHFNRAIKQRGLDALYITGPGHGGPGLVA
                     ASYLEGTYTETYPDIPPTVDGVQRLFKQFSFPGGIPSHVAPETPGSIHEGGELGYALS
                     HAYGAIFDNPTQLAFAVVGDGEAETGPLATSWHSNKFVNTQTDGVVLPVLHLNGYKIA
                     NPTVLARIPEEELGALMRGYGHTPLFFTGGFDDEDHIACHLRFAGILDEALDMIAEIK
                     SRPEGTDQERPLWPMIVFRTPKGWTGPKTIDGKRTEDSWRSHQVPLASARDTDEHLQD
                     LEDWLTSYRPEELFTDDGALKPEIAALAPEGTKRMSANPITNGGAVLKDLALPDFRQY
                     AVDVPHPGGSINESTRVLGSWLRDVVKANPDNFRIFGPDETASNRLQDVYEVTSKQWN
                     ADYLPTDAEDDHLAPEGRVMEMLSEHQCQGWLEGYLLTGRHGLMSSYEAFIHIVDSMF
                     NQHAKWLKVTNHIGWRRPVASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKAEIIR
                     VYLPPDTNTLLSTFDHCLRSRQYVNVVVAGKQPNPNWLNIEEAVKHCTRGIGIWHWAG
                     TEDDGDHPDVVLGCAGDIPTIEVLAAAQILREAVPELKVRVVNVVDLMRLQDEREHPH
                     GLSAREFDNYFTSDRPVIFAYHGYPWLIHRLAYRHDVDSHFHVRGFKEEGTTTTPFDM
                     LMRNDLDRFHLVMDVIDRVPSLGARYANLRQYMVDKRTMARAYAYEHGEDLPEVREWQ
                     WGDAGDMADRGQVDAALATGGDNA"
     gene            complement(5283..5495)
                     /locus_tag="PKU75_12265"
                     /pseudo
     CDS             complement(5283..5495)
                     /locus_tag="PKU75_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019311032.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            complement(5580..6134)
                     /locus_tag="PKU75_12270"
     CDS             complement(5580..6134)
                     /locus_tag="PKU75_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81699.1"
                     /translation="MADLRFLRMTPFAVEVRRRSHRSITVAQIHNPAGGWPRGQGRAA
                     LDDFLSGLDLPRAQIVLAGHSSGGHVALAAGADCGGIIALAPWLSGSEPVEHLRGSRV
                     TILHGDRDRVTDPARSAAYVRRLVAAGVSARFQQMAGAGHAMVQRPGQWHRAVADEAI
                     AILLPHMANPNGPTDFPFERPLGP"
     gene            6295..7188
                     /locus_tag="PKU75_12275"
     CDS             6295..7188
                     /locus_tag="PKU75_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPF81700.1"
                     /translation="METCCATKGGTSKVAEGHTIHALAARLNTAFAGQPVRVSSPQHT
                     FAAEAGRLNGHEMRQASAHGKNLLVAFADAPTLHVHLGMFGSFSVRAHKRAIRDGWLR
                     TEPPASGSERLRLLTLTHLGDLRRPMQCRLLDDDGVAALLDRLGPDPLAPEQDPDAAV
                     RVLHRSRREIGLLLVDQSVVAGIGNVYRAELLFRAGLSPWLPGSAVDLNLLRTLWSDA
                     TYLMSIATAAGWIITDTRQVEQAAPYLDRGERVPRWPKVYAVYGRAGQPCRRCGTPID
                     SAAQGRQRIFWCPRCQPFVDD"
     gene            7254..7676
                     /locus_tag="PKU75_12280"
     CDS             7254..7676
                     /locus_tag="PKU75_12280"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPF81701.1"
                     /translation="MSMSLSELSKLLRKLDICMLGTHGARGTLAARPMSNNKDVEYDG
                     TSHFFTWADSRMARDIRRDPGVLLTFTDPKGVFVAVQGTARITTDKKLMAEHWVPDVE
                     RWFADGVETAGICMIIVDANRISYWTYEHGDGEITMRK"
     gene            7681..>8867
                     /locus_tag="PKU75_12285"
     CDS             7681..>8867
                     /locus_tag="PKU75_12285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPF81702.1"
                     /translation="MDADVVVVGAGLAGLQCARTLTAAGLQVVVLEAADAVGGRVRTE
                     CIDGFLCDRGFQVLNPAYPAVRRSVDLEALELQQFAPGVLVRAGDGLRVVADPVRAPQ
                     LLRSTLSSGLVRPGELLALTRWLAPILVAPQRNSRSRDERLTLSLDNAGVTGPLRRVM
                     DRFLAGVLVDSWGTSSANFTRLLLRSFALGAPGLPRDGMSALPQQLASTLADVRLNTA
                     VRSVHPSTTGDGSHTVTTDGGTITGRAVVIASDPASAAHLAGLPPTPMKGLVTWWFNA
                     PQAPDHRPLLVVDGRHGPDDGPPGPVWNTAVVTAAAPSYATGGRVLIQATTLLDRPDG
                     DSDERTVRAHLGDIYQCATGDWEVVVRHRIPRALPASPPPLHHHQPARVAPGLYVCGD
                     HRDT"
BASE COUNT         1409 a         2856 c         3120 g         1382 t
ORIGIN      
        1 gccattgagg cctgtagggg cattttcaca ccccaaaggg tggtttccga gtcattcgct
       61 ggttgggcgg atcctctgcc gcttttcgac cccgcgatgt ccctatggtc gtggcccagg
      121 cccgctggcg cgcagtgtcg attggatgct cccagggggt gccaccgcca gaaaggcagg
      181 cgaatgtcag attccgtgtt cgaccagaac aaagtggttg accagaacta cgtgattgac
      241 caggacaagc tcccgggcgg ccagccccag cagtcgacct cgtcgcaagc cgttgacgag
      301 gccaagagcg ttgccgccga cgcggcgtcc agcggcaagg ccgtcgccgg ggccgccaag
      361 gaggaggttg cgcaggttgc cggggaagcc aagaaccatg ctcaggacct ctatcaccag
      421 gcccgatcgg aggtcagctc gcaggctgcc acgcagcagg accgggtggc ggggctcttg
      481 cggctgctga cctcagagct gcaggaaatg ggccgcagca gtcagagcgg tatcgccagc
      541 ggcctggtga gccaggctgc cgaccaaacc gagcgggtcg ccggatggct ggagtcacgg
      601 gacccgagtg acctgctcga ggacatccgg cggtatgcac gccgcaatcc cggcaacttc
      661 ctcgccggag cagcgcttgc cggattcctc ggcggccgga tcacgcgcag cctggcgagt
      721 caggacgacg acgcggacca gcagtatgtc cagcggcagc ggcccgtgac cacggtgccg
      781 gcaggcgcgg tgacgacaca gcacataccc agtgccgtcc cggcatacgg tggctcgccc
      841 accccggcca cggcgccgaa cccggcgagc gtccgcacgg ggaccacccg agaggccggc
      901 ggggagctgc cccgatgagt caagtcggcc acgaagggtc gaccgcagcc ggcccgccgg
      961 tccgtgagcc ggccgagatc ggtgaggtgc gcccggcaga gcagatcgag ggtgcccatg
     1021 acacctacga caagggcgag atcggctcca tcggcgcatt actgggcgac atcgccggcg
     1081 acgtcacgac cttgatgcgt caagaagtgg cgctggccaa agccgaggtg cgccagtcgg
     1141 ccaccgacgc gggcaagggc gcggggatgc tggcgggcgc gggcgtcgct ggccacctgg
     1201 ctctgctctt cctgtcgatc gccctgtggt gggcgctcgg gtcgtggtgg gacaacttcg
     1261 gcgtcgcggc tttggtcgtc ggcgtcatct gggcgctcat cgccgccgtg ctggcggcgc
     1321 gtggtcgcac cgaactcaag aacgtcactg gagtgccgcg cacagtcgag accgtcaaac
     1381 aggtcccgga tgcgctgaag ggaaacgagg atccgcgatg agcaatgaca tccccacgat
     1441 cgacagcaac gacccggagc aattgcgccg ggacatcgag cagacccgag tcaacctgag
     1501 ccagaacgtc aacgccctgg gcgaggcggt cgaacccggc gccgttgcca agcgccaggt
     1561 cgagaaggtg caggacaagg tggtcggcgc cggccgcggg ctgaaggaga aggtcatggg
     1621 cagcgacgac gactatggag actccagcgg cctgaccggg aaactgagcg acgccggaga
     1681 cacggtcggc gacatggccg cgtcggcacg cgcgcaggtg gagaacgcgc cgtccgccgc
     1741 ccggcgccgc acccgcggca atccgctcgc ggccggcctg gtcgctctcg gggccggttg
     1801 gttgttgggc tcgttgctgc ccgcgtccga gaaggaacgg caactggccg cgaccgccaa
     1861 ggacgccgtc gaggagaagg gccagcctct gctcgaggag gccaagtccg ttgtgcaaga
     1921 ggctgccgag aacctcaagg agccggcgca ggacgccgcg aacgccctga aggagagcgc
     1981 tcaagagagc gtcgaaaagg tcaaggccga gggccagggc gctgcccaaa ccgtcaagga
     2041 aagcgcgcag gagtcgaagg acgcggtgca gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnncgctgc ccaaaccgtc aaggaaagcg cgcaggagtc gaaggacgcg
     2221 gtgcagcgca acagctgacg ccgaggtcgt cgtcggcgtc aacctgggtc gtcacgcagt
     2281 gatccagtgc gtcgccgttt cgccgacgcg aaggtgggcg ctctcgacaa gatggtcgcg
     2341 cggggcggcg gactcggttc accgccccgc gccgcacgct aggagacgca gccctgctca
     2401 cggcatacgg caccgccctg gccgtctatt ccgggtatgc cgcggcgagc ctcgaggcgc
     2461 gggcggaccg gtgccggcgg gacaggtgcc gtcaggcgtt gtccccgccg gttgccaggg
     2521 cagcgtccac ctgaccgcgg tcggccatgt cgcccgcgtc gccccactgc cactcgcgca
     2581 cctcgggcag gtcctcgccg tgctcgtagg cataggcccg agccatcgtg cgcttgtcga
     2641 ccatgtactg gcgcaggttc gcatagcgcg cacccagcga cggcacccgg tcgatgacgt
     2701 ccatcacgag gtggaaacgg tcgaggtcgt tgcgcatgag catgtcgaag ggcgtcgtcg
     2761 tcgtgccctc ctccttgaac ccgcgcacgt ggaagtggga gtcgacgtcg tgccggtagg
     2821 ccagccggtg gatgagccac gggtagccgt gataggcgaa gatcaccggg cggtcgctgg
     2881 tgaagtagtt gtcgaactcg cgcgccgaca ggccgtgcgg gtgctcccgc tcgtcctgca
     2941 accgcatcag gtcgacgacg ttgacgacgc ggaccttcaa ctccgggacc gcttctcgca
     3001 ggatctgggc cgcagcgagc acctcgatcg tcgggatgtc gccggcgcag ccgagcacga
     3061 catcggggtg atcgccgtcg tcctccgtgc ccgcccagtg ccagatgccg atgccgcggg
     3121 tgcagtgctt gacggcctct tctatgttga gccagttggg attcggctgc ttgccggcga
     3181 cgacgacgtt gacatactgc cgagatcgca ggcagtggtc gaaggtcgac agcaaggtgt
     3241 tggtgtccgg cggcagatag acccggatga tctcggcctt cttgttgacc acgtgatcga
     3301 tgaatcccgg gtcctggtgg gagaacccgt tgtggtcctg ccgccacacg tgcgagctga
     3361 gcagatagtt caaggacgcg acggggcgcc gccagccgat gtggttggtg accttgagcc
     3421 acttggcgtg ctgattgaac atcgagtcga cgatgtggat gaacgcctcg tacgaactca
     3481 tcagcccgtg ccggccggtg agcagatagc cctcgagcca gccctggcac tggtgctcgg
     3541 agagcatctc catcacccgg ccctcggggg cgaggtggtc gtcctccgcg tccgtcggca
     3601 ggtagtcggc gttccactgc ttcgacgtga cctcgtagac gtcctgcagc cggttggagg
     3661 cggtctcgtc cggtccgaag atgcggaagt tgtccggatt ggccttgacc acatcgcgca
     3721 gccagctgcc gagcacgcgg gtcgactcgt tgatcgagcc acccgggtgg gggacgtcca
     3781 ccgcatactg ccggaagtcc ggaagggcga ggtccttcag gaccgcgccc ccgttggtga
     3841 tcgggttggc gctcatccgc ttggtgccct cgggggcgag cgcggcgatc tcgggcttga
     3901 gggcaccgtc gtcggtgaag agttcctcgg gccggtatga cgtgagccag tcctcgaggt
     3961 cctgcaggtg ttcgtcggtg tcgcgggcgc tggccagggg cacctggtgg ctgcgccagg
     4021 agtcttcggt gcgcttgccg tcgatggtct tcgggccggt ccagcccttg ggggtccgga
     4081 agacgatcat cggccagagc gggcgctcct gatccgtgcc ctccggccgg gacttgatct
     4141 cggcgatcat gtcgagcgcc tcatcgagga tgccggcgaa gcgcaggtgg caggcgatgt
     4201 ggtcctcgtc gtcgaagccg ccggtgaaga agagcggcgt atgcccgtag ccgcgcatga
     4261 gcgcgccgag ctcctcctcc ggaatacgcg ccagcacagt gggattggcg atcttgtagc
     4321 cgttgaggtg cagcaccggc aggacgacgc cgtcggtctg ggtgttgacg aacttgttgg
     4381 agtgccaact cgtcgcgagc ggcccggtct ccgcctcgcc gtcgccgacc accgcgaaag
     4441 ccagctgggt cgggttgtca aagatcgccc catacgcatg cgagagcgcg tagccgagtt
     4501 caccgccctc gtggatcgag ccgggggtct cgggggcgac gtgcgagggt atgccgccgg
     4561 ggaaggagaa ctgcttgaag agccgttgaa cgccgtcgac ggtcgggggg atgtccgggt
     4621 aggtctcggt gtaggtgccc tcgaggtagc tggcggcgac caggcccggg ccgccgtgcc
     4681 cgggcccggt gatatagagg gcgtccaggc cgcgctgctt gatcgcgcgg ttgaagtgcg
     4741 catagaggaa ggtcaggccg ggcgtggtcc cccagtggcc gagcagccgc ggcttgacgt
     4801 gatcgcgggt cagcggctcg cgcagcagcg gattgtccag caggtagatc tggccgacgg
     4861 acagatagtt ggccgcccgc caccaggcat caagcagccg ggcttcgtcc tcggtcatcg
     4921 ggctgctcgc gccgttgggc cagctgctcg gttcggtcat cagtcctcct gggtcgagtg
     4981 aaaggaatcc cgtccattct cgcccgagcg gccgccgcac gggacgcggg gttgatgaac
     5041 acttggattt cgggtgctcc ccgcccgacg tggcccgctg cgtccgcgcg caccgtcgcc
     5101 gcgggtcatg cggcggacaa gtctcgagac cgctccgggc ggtatgccgt gggatggcgc
     5161 cgtggaggtc gagcgagcgc cgcagatgtg gtcgttgcgc cgcaagcacg gcccagcatc
     5221 cacgagcgcg tgacggccgg tcgaggacta cggcgcccat gtcggccgag acgcaacgcc
     5281 atgctggaaa tgcgctcggc cagcacatcg gccatgcctc gaacaccctt gatctgccgg
     5341 tccagcatct cgtggacccc gatgaaccct gggcccacga aatttcagtg ggcttgcttg
     5401 ggcgctaagg cgaggtcgtt gagcgcatgc gggcgcacct gcagctgggc tgccacctgg
     5461 tgccccaccg aaagtgtccg gccggggacg gtgatcggcg cgtccgcctc ggacgacaca
     5521 gcggtggtgg atcagttggc attgcgggtg gcgcgctgcg tggttgccat cgccgcaggc
     5581 tatggcccga gcggacgctc gaacgggaaa tcggtgggtc cattcgggtt tgccatgtgg
     5641 gggagaagaa tggcaatcgc ttcgtccgcc acggcgcgat gccactggcc cggtctctgc
     5701 accatggcat ggcccgctcc ggccatctgc tgaaagcggg cggagacgcc cgctgccacg
     5761 agccgccgga cgtatgccgc ggaccgggcc ggatcggtga cgcgatcgcg gtcgccgtgc
     5821 aggatggtga cccgagaacc gcgcaggtgc tcgaccggtt cgctgccaga cagccacggg
     5881 gcaagcgcga tgatgccgcc gcagtcagcc ccggctgcca gggccacgtg cccccccgac
     5941 gagtgcccgg caaggacgat ctgcgctcgt gggagatcaa ggccgctcaa gaagtcgtcc
     6001 agggctgctc ggccctggcc acggggccag ccaccagccg ggttgtggat ctgggcgacc
     6061 gtgatcgacc gatggcttcg gcgacggacc tcgaccgcga agggagtcat ccgcaggaag
     6121 cgcaggtcgg ccatggccga ggtgctgggt cgccggccgg ttccaccgtg caggagcacg
     6181 aggaccggcg ccgagccatc tcttgtccca aaccagcgca cgcccaggct catgaggtct
     6241 ccggcttcac cgacgcacgg tatcccggat gatgggggag ttatccgatg cgtcgtggag
     6301 acgtgttgcg cgacgaaggg tggcacgagc aaggtggcgg aagggcatac gatccacgcc
     6361 ctggcggccc gcctcaacac ggcgttcgcc ggacagccgg tccgcgtgag ttcaccgcag
     6421 cacaccttcg ccgcggaagc cgggcggctg aacgggcacg agatgcgcca ggcgagcgct
     6481 cacggcaaga acctcctggt ggcttttgcc gacgcaccga ctctgcacgt gcatctgggc
     6541 atgttcgggt ccttctcggt ccgtgctcac aagcgggcga tcagggacgg atggttgcgc
     6601 accgaaccgc ccgcgtcggg ttccgagcgg ttgcgcctcc tgacgctgac gcacctcggc
     6661 gacctgcgcc ggccgatgca gtgccgcctc ctcgacgacg acggcgttgc cgctctgctt
     6721 gaccgccttg gcccggatcc cctggccccg gagcaggatc cggacgccgc cgtgcgtgtg
     6781 ctgcatcgct cccgccgtga gatcgggctg ctgctggtgg atcagtcggt cgtcgcgggc
     6841 atcgggaacg tctatcgcgc cgaactgctc ttccgggccg ggctgtcgcc gtggttgccg
     6901 gggtccgcag tcgacctcaa cctcctacgc accctgtggt ccgacgccac ctacctgatg
     6961 tcgatcgcga ccgcggcggg gtggatcatc accgacactc gccaggtcga acaagccgcc
     7021 ccttatctag atcgaggcga acgggtcccg aggtggccca aggtgtatgc ggtctacggc
     7081 cgcgccggcc agccctgccg acgctgcggc acaccgatcg attctgcagc gcagggtcgg
     7141 caacgcattt tctggtgtcc tcgctgccag cctttcgtcg atgattagcg actagggtgc
     7201 ggacagactc ggcgtgcagc tcaccgagcg tccccgagag aggcccagca cccatgtcca
     7261 tgtccctcag tgagttgtcg aaactgttgc gcaagctgga tatttgcatg ttgggcaccc
     7321 atggagcccg gggcacgctc gccgcccggc cgatgagcaa caacaaagac gtcgagtatg
     7381 acggcacctc ccacttcttc acctgggcgg actcccggat ggcccgggat atccgccgcg
     7441 atcccggtgt gctcttgacg ttcacggatc cgaagggggt gttcgtggcg gttcaaggga
     7501 ccgcaaggat cacgaccgac aagaagctca tggccgagca ctgggtcccg gatgtcgagc
     7561 gctggttcgc cgatggtgtg gagaccgcgg gcatttgcat gatcatcgtg gacgccaacc
     7621 ggatcagcta ctggacctac gagcacggcg acggcgaaat cacgatgagg aagtgacgtg
     7681 atggacgcag atgtcgtcgt cgtgggggcc ggactcgccg gtctgcagtg cgcgcgtacg
     7741 ctgacggcag ccggcctcca ggtcgtcgtc ctcgaggcgg cggacgcggt cggcggtcgc
     7801 gtgcgcacgg aatgcatcga cggattcctg tgcgatcgcg gtttccaggt gcttaacccg
     7861 gcgtatccgg cagtccgtcg ctcggtggat ctggaggcgc ttgaactgca gcaattcgcg
     7921 ccgggggtct tggtccgtgc gggtgatggg ttgcgagtcg ttgcggaccc cgtgcgggcc
     7981 ccgcagctgc tgcgatccac gttgtccagc gggctcgtca ggcccgggga gctgctcgcg
     8041 ctgacacgct ggttggcgcc gattctggtt gcaccgcagc ggaattcgcg atcccgcgac
     8101 gaacgtctga cgctgtctct agacaacgca ggagtgacgg ggccgctgcg gcgggtgatg
     8161 gatcggttcc tggccggtgt gctggtggac agctggggga cctcgtccgc gaacttcacg
     8221 cggctgctgc tgcgcagctt tgcgctcggt gcaccggggc tgccgcggga cgggatgagc
     8281 gcgttgccac agcagctggc atcgacgctg gccgacgtgc ggctcaacac ggccgtgcgg
     8341 tccgtgcacc cctcgacgac gggagacggc agccacaccg tgacgaccga cggcgggacg
     8401 ataaccggcc gcgctgtggt gatcgccagt gacccggcga gtgccgcgca cctcgcggga
     8461 ctgccaccaa cgccgatgaa gggcctggtc acgtggtggt tcaacgctcc gcaggcgccg
     8521 gaccaccggc cgctgctcgt ggtcgacggc cgccacggac cggacgacgg accgccgggg
     8581 ccggtctgga acaccgcggt cgtcaccgcc gcggccccgt cgtatgcgac cggcgggcgc
     8641 gtgctcattc aagccaccac gctgctcgac cggcccgacg gcgacagcga cgaacggacc
     8701 gtacgagcgc acctgggcga catctaccag tgcgccaccg gtgactggga ggtcgtcgtt
     8761 cgccaccgca taccgcgagc tctgcccgcc tcccctccgc cgctgcacca tcaccagccc
     8821 gcccgggtcg cccccggttt gtatgtctgc ggcgaccacc gcgacac
//
LOCUS       DAOPJG010000145         8701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8566_length_8701_cov_6.716516, whole genome shotgun sequence.
ACCESSION   DAOPJG010000145 DAOPJG010000000
VERSION     DAOPJG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8566_length_8701_cov_6.716516"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKU75_12290"
     CDS             <1..300
                     /locus_tag="PKU75_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HPF81703.1"
                     /translation="RDHGGFTEPHAWESMVRDWHLEGLAVRPVHRMHGHTGFLITTRR
                     LAPGVTPPLRKRRPGRGYDADAAEEWTAEDLGERVPSDKRVRRTARSIGDGPRLP"
     gene            509..2140
                     /gene="arc"
                     /locus_tag="PKU75_12295"
     CDS             509..2140
                     /gene="arc"
                     /locus_tag="PKU75_12295"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HPF81704.1"
                     /translation="MTEDTTLELRRVRSQMATMSAQNERLVRTLKDAREQLVNLKAEV
                     DRLANPPAAYGIILQVHDDGTADILTGGRKMHVAVSPTIPPEDLVGGRDVRLNEALNV
                     VAVCGYERVGEVVLVKEMIEPDRVLVVAHADEERVCRMAESLADVPVRVGDALLLEPR
                     SGFVFERIEKAEVADLVLEEVPDISYEDIGGLRGQIEAIRDSVELPYLHPDLFTEHQL
                     KPPKGVLLYGPPGCGKTMIAKAVAASLARKVAEKEGKPLSKSYFLNIKGPELLNKYVG
                     ETERHIRLIFHRAREKSNDGTPVVVFFDEMDSLFRTRGSGVSSDVETTIVPQLLAEID
                     GVEGLENVIVIGATNREDMIDPAILRPGRLDVKIKIERPDAESARDIFRKYLTADLPL
                     HADDVAENGGSAEATVEAMIEAAVARMYSEDEDNRFLEVTYAGGDKEILYFKDFNSGA
                     MIQNVVDRAKKSAIKDFLTTGQRGIRVEHLLSSCLDEFKENEDLPNTTNPDDWARISG
                     KKGERIIYVRTLVSTKSGTEPGRTIDTVTNTGQYL"
     gene            2563..3360
                     /locus_tag="PKU75_12300"
     CDS             2563..3360
                     /locus_tag="PKU75_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81705.1"
                     /translation="MTLTSKVPLAFARASAAAVLVLPRISPWPTRVQILGDPWNGADN
                     GHRHRGSRAGMSRCIGACDRLMSLHGLLITPVARTVVDLARSDRLDNALAAGDQALRR
                     EWCSRDEIAEEVAAIPRRARGRKQAQLFFNLVDGAAHTAGESLSRLGMFRLNVPKPIL
                     QYPLEDADGAFGFADFGWRIGADLIIGEFDGKVKYAVDPELGPKAAQEALWREKQRED
                     RIRRQADVVRWGMKEATDLRLLAQRLAEHGILPTAHNTWFDPPAHPE"
     gene            complement(3605..4585)
                     /locus_tag="PKU75_12305"
     CDS             complement(3605..4585)
                     /locus_tag="PKU75_12305"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPF81706.1"
                     /translation="MRDIVVFSGTSHRTLAGRICNALGVPLSPSLTTRFSNDCLQVQL
                     QANCRQRDVYLIAPVVPPTQESLMELLLMIDAAKGASAAQITAVIPHFAYARSDKKDA
                     SRISMGGRLVADMIVAAGAHRVLTMTLHAPQVHGFFSIPVDHLTAIGILADHFRAQDL
                     TDTVVVSPDLGNAKTASQFARLLRLPVAAGSKVRTADDKVVIDMIVGDVAGKKAIVLD
                     DEIATGGSIVELMDRLKDFGCTEAAVACTHGLFTGQAVERLANHPMITEMVTTDTVPR
                     PHQSWEALQVRSVAGLFAEAIGRIHAGESISSLFDGVDPTHAPPQATLFP"
     gene            4741..5130
                     /locus_tag="PKU75_12310"
     CDS             4741..5130
                     /locus_tag="PKU75_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81707.1"
                     /translation="MARDTAYDRIRDELGVAQAAPPAQALPHLRAVVDQVTELIDEAL
                     AAAVLSGDESLRSAGAAAGLTENAVGPRLARTPALAPYANAAGRVTADGVRRAMYDAE
                     RGAVPPPSDQGSSSAPMRFKARRRSST"
     gene            5127..5765
                     /locus_tag="PKU75_12315"
     CDS             5127..5765
                     /locus_tag="PKU75_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPF81708.1"
                     /translation="MTDAALTHIYFLLDRSGSMQSIKSDTEGGFAAFVEEQRKTSGRC
                     RVTLAQFDTTYDVVYQDRDLAEVPPLDLQPRGMTALLDAIGRLVTDAGRRLAALPEEQ
                     RPGTVVVAIMTDGMENSSHEWTHPAVKSLIEQQEQGYNWQFLYMGADQDAIEVGNSIG
                     IAAQNSLTYSRGNVDHAMAAAASMVGDLRRARAASPAAKLRGYSDEEREASR"
     gene            5850..7373
                     /gene="dop"
                     /locus_tag="PKU75_12320"
     CDS             5850..7373
                     /gene="dop"
                     /locus_tag="PKU75_12320"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HPF81709.1"
                     /translation="MGVERVMGIETEYGISLTGAPHANPMVLSGQVVTAYAQAHGVRA
                     ARGSWDYADEAPLRDARGFEMGRGVAHPSQLTDEWGSDDDPTLANVVLTNGARLYVDH
                     AHPEYSSPEVTTPRAGLTWDRAGELVMQEAVRRLAASPPGINLYKNNTDGKGSSYGTH
                     ENYLMRRSTPFAQIVTHLTPFFVARQVMCGSGRVGIGVDSRVPGYQIAQRSDFFETEV
                     GLETTLKRPIINTRDEPHATADKYRRLHVIIGDANHCDVAGLLKLGSTSLVLELIEAD
                     AVTDRLSVRHPVATLQAISHDPTLTHVIELEDGRKLTAVQLLWRYYEMVDARLDQTGE
                     GEQPHTAEVMRWWADVLGKLERDPMSAAAEVDWVGKLRVLQAYQEREGLAWNDPKLKA
                     IDIQWSDVRPDKGLFNKLRAAGRFTELIPEEEARRAVTAPPEDTRAYFRGRCLERFPD
                     NVVAASWDSLILEVPGVRALRRVPMMEPLKGTRDAVGDLLDDASDVGELLAALAAAD"
     gene            complement(7457..8005)
                     /locus_tag="PKU75_12325"
     CDS             complement(7457..8005)
                     /locus_tag="PKU75_12325"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPF81710.1"
                     /translation="MTTPKSGSVRTVYRSAGPPVLQVDLTDVTIAGEVQEWHTVTAQH
                     GQPGAVVVALHNNRILVGEHWRLTTGRRELEFPRGFGEPSEGAADTASRELREETGLV
                     AKSVAVLGTVFADTGLLTNPIHVVQAHVDRVDSEKVDRDPEFEDLYWLDAREVRSRIA
                     DGSLRDAISLAAFALWQARQAD"
     gene            complement(8010..>8701)
                     /locus_tag="PKU75_12330"
     CDS             complement(8010..>8701)
                     /locus_tag="PKU75_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81711.1"
                     /translation="WREAADRWAPPLLRDKRSAAIVAELREEPKRTNALRRIAEASLT
                     EQQGRHLIAWYETVYADFVARNNGADWIDMTGGRFSDTPVTRSAGVRARMITLRGAAP
                     IHLGTMPRSQYELLCYRARDAIALWQSEPTPRSADRIAYAIERAQSQDDLDNDRRDLA
                     VGLAATLIVTTLLLGLEAMAGSLPIGIWFVPVVTLALTMALELRSARAPIRAVRRSEL
                     RSTIHLTTGGG"
BASE COUNT         1477 a         2977 c         2941 g         1306 t
ORIGIN      
        1 cgcgatcacg gcggcttcac cgagccgcac gcgtgggagt cgatggtgcg cgactggcac
       61 ctcgaaggac tcgcggtgcg ccccgtgcac cggatgcacg ggcataccgg cttcttgatc
      121 accacccgcc ggctcgcacc cggcgtcacc ccgcccctgc gcaagcggcg ccccggccgc
      181 gggtatgacg cggacgccgc ggaggagtgg accgccgagg acctcgggga gcgggtgccc
      241 agcgacaagc gggtgcgccg caccgcccgg tcgatcggcg acggacctcg cctgccctga
      301 taaccccacg aagcgtcctc gcttcgccgc actacctcgg ggggagccac gaaccgtggt
      361 ctgcagccct gaatccccga atcggtccca gggcgtggga cggtcccgag ccgtgacgaa
      421 ttcctcccac gctttgtcgg atgcgggagt tagagtcgaa cagacgccgc tcccgcgacg
      481 ggggcagcag gatcgggagg aggctgtcgt gaccgaagac acgaccctcg aactgcgccg
      541 tgtgcgctcg cagatggcca ccatgtccgc ccagaacgag cgcctcgtgc gcaccctcaa
      601 ggatgcccga gagcaactcg tcaacctcaa ggccgaggtc gaccggctcg ccaatccgcc
      661 cgcggcatac ggcatcatcc tgcaggtcca cgacgacggc accgccgaca tcctcaccgg
      721 cggccgcaag atgcacgtcg ccgtcagccc gaccatcccg cccgaggatc tggtgggcgg
      781 gcgcgatgtc cgcctcaacg aggccctcaa tgtcgtcgcc gtctgcggtt atgagcgcgt
      841 cggtgaggtc gtcctcgtca aggagatgat cgagccggac cgggtgctcg tggtcgcgca
      901 cgccgacgag gagcgggtat gccgcatggc cgagtccctc gcggacgtgc cggtccgcgt
      961 cggcgacgca ttgctgctgg agccgcgcag cgggttcgtc ttcgagcgca tcgagaaggc
     1021 cgaggtcgcg gacctggtgt tggaggaggt ccccgacatc tcctatgagg acatcggcgg
     1081 cctgcggggt cagatcgagg cgatccgcga ctcggtcgag ctgccctatc tgcatccgga
     1141 tctgttcacc gagcaccagc tcaagccgcc caagggcgtg ctgctctacg gcccgcccgg
     1201 ctgcggcaag acgatgatcg ccaaggcggt tgccgcctcg ctggcgcgca aggtcgccga
     1261 gaaggagggc aagccgctct ccaagagcta cttcctcaac atcaagggcc cggaactgct
     1321 caacaaatat gtcggcgaga ccgagcggca catccggctg atcttccacc gggcgcggga
     1381 gaagtccaac gacggcacgc ccgtggtggt cttcttcgac gagatggact cgctcttccg
     1441 cacccgtggg tccggcgtgt cgagtgacgt cgagacgacg atcgtgccgc agctgctggc
     1501 cgagatcgac ggggtcgagg gtctcgagaa cgtcatcgtc atcggcgcga ccaaccgcga
     1561 ggacatgatc gacccggcga tcctgcggcc cggccggctc gatgtgaaga tcaagatcga
     1621 acgcccggac gcggagtcgg cgcgcgacat cttccgcaaa tacctcaccg ccgacctgcc
     1681 gttgcacgcg gacgacgtcg ccgagaacgg cggctcggcc gaggccacgg tggaggcgat
     1741 gatcgaggcc gcggtggcgc gcatgtattc cgaggacgag gacaaccgtt tcctcgaggt
     1801 cacctacgcc ggcggcgaca aggagatcct ctacttcaag gacttcaact ccggggcgat
     1861 gatccagaac gtcgtcgacc gggccaagaa gtcggcgatc aaggacttcc tgaccaccgg
     1921 ccagcggggc atccgggtcg agcacctgtt gtcctcgtgc ctggatgagt tcaaggagaa
     1981 cgaggacctg cccaacacga ccaaccccga cgactgggcg cggatctcgg gcaagaaggg
     2041 cgagcggatc atctacgtcc gcacactcgt ctccaccaag tccggcaccg agcccggccg
     2101 caccatcgac acggtcacca acaccgggca gtacctctag ttattggtga ctcgggccag
     2161 gttttcgttc tcgggccggc ctatttccag gccccaaaac gaaagccgag cccgagttga
     2221 tggcctggtt gcggtccgcc gggcttgggc ggggtggtgg gggcgtggcc gatggcgcgc
     2281 cgacgtgacg ggctatccac tggctatcca caggcctact ggtggaccca cgacaggacg
     2341 accgttctgc gcgacagtga caacatgttc ggtccaccaa ttcctctgcc gttcgacccc
     2401 agcctgctcc ggctcggacg cgacctgcga gctcgcggca tcgagccatg cgcgctccca
     2461 acagagtggt ttcgggtccg gcgtggcgta tggatcgcga cggtcgtctg gcaggaactc
     2521 accccggagt atcagcacgc tgcacgggtg cacgcagcgg ccatgacctt gacctcgaag
     2581 gtgccgctgg cgttcgcccg cgccagcgct gccgccgtct tggtcctacc ccgcatcagc
     2641 ccttggccaa cgcgcgtcca gatcctgggc gacccgtgga atggcgcgga caacggccac
     2701 cgccaccggg gcagtcgcgc cggcatgtcc cggtgcatcg gcgcctgcga ccggttgatg
     2761 tcgctacatg ggctactgat caccccggtt gcgcgcaccg tggtcgatct cgcccggtcc
     2821 gaccggctcg ataatgcgct cgccgccggg gatcaggcgc tgcgccggga gtggtgctct
     2881 cgggacgaga tcgccgagga ggttgccgcc ataccgcggc gcgcgcgggg gcgcaagcag
     2941 gcgcaactct tcttcaatct cgtcgacggc gccgctcaca cggccggcga gagtctgagc
     3001 cggttgggga tgttccggtt gaacgtgccc aagcccatcc tgcagtaccc cttggaggac
     3061 gccgacggtg cattcggatt cgccgacttc gggtggcgca ttggtgccga cctgatcatc
     3121 ggtgagttcg acggcaaggt gaagtatgcg gtcgaccccg agctcggacc gaaagcggcc
     3181 caggaggcct tgtggcgcga gaagcagcgc gaggaccgca tacgcaggca agcagacgtc
     3241 gtgcgctggg ggatgaagga agccacggac ctgcgtctcc tggcacagcg cctcgctgag
     3301 cacgggattc tcccgacagc gcacaacacc tggtttgatc cccccgcaca cccggaatga
     3361 cactgtcggg cgggtcgggg gacgccggga cccccaccgc ccagccgagg cccgaccgac
     3421 cgccacaaag ccacaaactc gggctcggct ttcgttctcg ggcctggaaa taggctggcc
     3481 cggggacgaa aacctggccc gtgtcaccga caacccggcc cgacaacgac aaccaggccc
     3541 gacaacgaca accaggcccg acaacgacag cccggcccgg cagccaggcg ccccggcggc
     3601 cgcctcaggg aaagagtgtc gcctggggcg gcgcgtgggt cgggtcgacg ccgtcgaaga
     3661 gggaggagat cgactcgccg gcgtggatgc ggccgatggc ctcggcgaag aggccggcga
     3721 ccgagcggac ctggagagcc tcccacgact ggtggggccg ggggacggtg tcggtcgtga
     3781 ccatctcggt gatcatcggg tggttggcga gacgctcgac ggcttggccg gtgaagaggc
     3841 cgtgggtgca ggcaacggcc gcctcggtgc acccgaagtc cttgagccgg tccatcaact
     3901 cgacgatcga gccaccggtc gcgatctcgt cgtcgaggac gatggccttc ttgcccgcga
     3961 cgtcgccgac gatcatgtcg atgacgacct tgtcgtccgc cgtgcgcacc ttcgacccgg
     4021 ccgccacggg cagccggagg agacgcgcga actggctcgc cgtcttggcg ttgccgaggt
     4081 cgggggagac gaccacggtg tcggtcaagt cctgggcgcg gaagtggtcg gcgaggattc
     4141 cgatggccgt caggtggtcg acggggatcg agaagaagcc atggacctgc ggcgcatgca
     4201 gggtcatcgt cagcacccgg tgggcgccgg cggcgacgat catgtcggcg accagccgcc
     4261 cgcccatcga gatgcgggag gcgtccttct tgtcggagcg agcgtaggcg aagtggggga
     4321 tcaccgccgt gatctgtgct gccgatgcgc ccttggccgc gtcgatcatc aagagcaact
     4381 ccatgagcga ctcctgcgtc ggcggcacga ccggcgctat cagatagacg tcgcgctggc
     4441 ggcagttcgc ctgcaactgc acctgcaggc agtcattgct gaatcgtgtt gtgagactag
     4501 gggaaagggg cactccgagc gcattgcaga tgcgcccggc aagggtgcgg tggctcgtcc
     4561 cggagaacac cacgatgtcg cgcatacgct cgccgttcct cgatcgccgg catgggggct
     4621 gccacccagc ctagtgcgcg gcggcccgcc cggacgccgc cggccggctc gagccgggcc
     4681 cgcagaaacc gcggggctgc agatagtgcg gggccgcaat aactgcggta tgatggtttc
     4741 atggcgaggg atacggcata cgaccggatc cgagacgaac tgggcgtcgc gcaggctgca
     4801 ccgccggcgc aggcgctgcc gcacctgcgg gccgtcgtcg atcaggtcac cgagctgatc
     4861 gacgaggcgc tcgccgcggc ggtcctgtcg ggggatgaat ccctgcgctc ggccggggcg
     4921 gcggccggcc tgacggagaa cgccgtcggg ccgcggctcg cgcgcacgcc cgctctcgcg
     4981 ccctatgcca acgcggcagg ccgggtcacc gcggacggcg tgcgccgggc catgtatgac
     5041 gcggaacggg gagccgtgcc ccctccgtca gaccagggga gcagttctgc gccaatgcga
     5101 ttcaaggcac gacgaaggag ttcgacatga ccgatgccgc cctcacccac atctacttcc
     5161 tcctcgaccg ctccgggtcg atgcagtcca tcaagtccga caccgagggc gggttcgcgg
     5221 ccttcgtcga ggagcagcgc aagaccagcg gccgctgccg ggtcacgctc gctcagttcg
     5281 acacgaccta cgacgtcgtc taccaggacc gcgatctggc cgaggtcccg ccgctcgacc
     5341 tgcagccgcg cggcatgacc gccctcctcg acgccatcgg ccggctcgtc accgacgccg
     5401 gccggcgcct ggccgccctc cccgaggagc agcgccccgg caccgtcgtc gtcgcgatca
     5461 tgaccgacgg gatggagaac tccagccacg agtggaccca ccccgcggtc aagagcctca
     5521 tcgagcagca agagcagggc tacaactggc agttcctcta catgggcgcc gaccaggacg
     5581 ccatcgaggt cggcaactcc atcggtatcg ccgcccagaa ctccctgacc tacagccgcg
     5641 gcaatgtcga ccacgccatg gccgccgcgg cgagcatggt cggcgacctg cggcgtgccc
     5701 gggccgcgag cccggccgcc aagctgcgcg gctattccga cgaggagcgg gaggcctctc
     5761 gctgaagccg ggtgacatct cgacgcccgt gaattgacac ctcgacggtg tggaaacgac
     5821 atctcgacgg ggtgcgtagg gttggggcca tgggcgtcga gcgggtcatg gggatcgaga
     5881 ccgagtacgg catctcgctc acgggggcgc cccacgccaa cccgatggtg ctgtcggggc
     5941 aggtggtcac ggcatacgcc caagcgcacg gcgtgcgcgc cgcccggggg tcctgggact
     6001 acgccgacga ggccccgctg cgcgacgcac gcggcttcga gatgggccgc ggcgtcgcac
     6061 acccgtcgca gctgaccgac gagtggggca gcgacgacga cccgacgctg gccaatgtcg
     6121 tgctcaccaa tggcgcccgg ctgtatgtcg atcacgccca ccccgaatac agctcgccgg
     6181 aggtgaccac gccccgtgcg ggtctgacat gggatcgcgc cggtgagctg gtgatgcagg
     6241 aggcggtgcg ccgcctcgca gcatcgccgc cggggatcaa cctctacaag aacaacaccg
     6301 acggcaaggg cagctcttac ggcacgcacg agaactacct catgcgccgc tcgacgccgt
     6361 tcgcgcagat cgtcacgcac ctgacgccgt tcttcgtcgc ccggcaggtc atgtgcgggt
     6421 cggggcgggt gggcatcggc gtcgactcgc gggtcccggg ctatcagatc gcccagcgca
     6481 gcgacttctt cgagaccgag gtcggtctcg agaccacgct caaacggccg atcatcaaca
     6541 cccgggacga gccgcacgcg acggcggaca agtatcgccg gctgcacgtc atcatcggcg
     6601 acgccaacca ctgcgatgtc gccgggctgc tcaagctcgg ctcgacgtcc ctggtgctcg
     6661 aactcatcga agccgacgcc gtcaccgacc ggctcagcgt gcgccacccg gtcgccactc
     6721 tccaggcgat ctcgcacgac cccaccctca cgcacgtgat cgagctcgag gacggccgca
     6781 agctcaccgc cgtccaactg ctctggcgct actacgagat ggtcgacgcc cgcctggacc
     6841 agaccggcga gggggagcaa ccgcataccg ccgaagtcat gcgctggtgg gccgacgtgc
     6901 tcggcaaact cgagcgcgac ccgatgagtg cggccgcgga ggtcgactgg gtcggcaaac
     6961 tccgtgtcct gcaggcgtat caggagcgcg aaggccttgc ttggaacgac ccgaagctga
     7021 aggccatcga catccaatgg tcggacgtgc gccccgacaa gggactcttc aacaagctgc
     7081 gggcggccgg gcggttcacc gagctgatcc cggaggagga ggcgcgccgc gcggtcaccg
     7141 cgccgccgga ggacacgcgc gcctacttcc gcggccgctg cctcgaacgc ttccccgaca
     7201 atgtcgtcgc cgcctcctgg gactcgctca tcctcgaggt ccccggcgtg cgagcgctgc
     7261 gccgggtgcc catgatggag cccctcaagg gcacccggga cgccgtcggc gacctgctgg
     7321 acgatgcctc cgacgtcggc gagctcctcg ccgccctggc cgccgccgac taaggcccgg
     7381 ctgctcgttc cgtcggccgc caatcagggc cgcgatggcc gagcgcggag cttcgcgggc
     7441 gaagactccg tgaaggttag tccgcctgcc gcgcctgcca aagggcaaac gcggcgagac
     7501 tgatcgcgtc tcgcagggag ccgtcggcga tgcgggatcg gacttctcga gcatcgagcc
     7561 agtaaagatc ctcgaactcc gggtcgcggt ctactttctc tgagtcaacc cgatccacgt
     7621 gcgcctggac gacgtggatc gggttggtca agagtccggt gtcggcaaag acagtaccta
     7681 gcacggcaac cgactttgcc accagtcccg tctcctcacg caactcccgc gaggccgtgt
     7741 cagcggcgcc ttcactaggc tccccgaagc cgcgggggaa ctccaactcc ctgcgaccgg
     7801 tcgtcaggcg ccagtgctcg ccgacgagta tgcggttgtt gtgtagggcc acgacgacgg
     7861 cgccgggctg tccgtgttgc gccgtaacag tgtgccattc ctgaacttca cccgcgatgg
     7921 tcacgtcagt caggtcgacc tgaaggactg gtgggcctgc gctgcggtat acagtcctga
     7981 ccgagccgct cttgggagtc gtcacgagtt catcctcccc cggttgtcag gtgaatggtc
     8041 gagcgcaact cacttcgtcg cactgctcga attggtgcgc gcgccgatcg caactccagc
     8101 gccatggtta aagccaacgt gacgaccggg acgaaccaga tgccgatggg gagcgaacca
     8161 gccatggctt cgaggcccag cagcagcgtc gtgacaatca aggtcgccgc caggccaacc
     8221 gcgagatcgc gtcggtcgtt gtcgaggtca tcttgactct gggctcgctc gattgcgtac
     8281 gcgatgcggt ctgcacttcg cggagtcggc tcggactgcc acagggcgat ggcgtccctt
     8341 gcccgataac agagcaactc atactgactc cgcggcatgg ttccgaggtg aatcggtgcg
     8401 gctcccctaa gcgtgatcat gcgggctcgg acgcctgccg acctcgtgac cggagtgtcg
     8461 gaaaagcgcc cgcctgtcat atcgatccag tctgcgccat tgttgcgggc cacgaagtcc
     8521 gcgtacacgg tttcgtacca agcgatcagg tgacgtccct gttgctcagt caagctggcc
     8581 tcagcgatcc tgcggagggc gttggtgcgt ttgggttcct cgcgcaactc ggcgacgatc
     8641 gcggccgagc gcttgtcgcg cagtagcggg ggagcccagc ggtcggcggc ttcgcgccag
     8701 g
//
LOCUS       DAOPJG010000146         8612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8671_length_8612_cov_8.042188, whole genome shotgun sequence.
ACCESSION   DAOPJG010000146 DAOPJG010000000
VERSION     DAOPJG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8671_length_8612_cov_8.042188"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKU75_12335"
     CDS             complement(<1..478)
                     /locus_tag="PKU75_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF81712.1"
                     /translation="MTVIDTEVQDGRARPQRGAKVLDAEQVTMRFGGLVAVNKVDFDV
                     HEGEIVGLIGPNGAGKTTFFNCLTGMYKPTSGTVKFYGEVLPPKPRAVVKAGMARTFQ
                     NIRLFGNMTALENVMVGRYCRTTSGAITSVLHGPKYRREEAESRSRAQELLDFVGLG"
     gene            complement(475..2181)
                     /locus_tag="PKU75_12340"
     CDS             complement(475..2181)
                     /locus_tag="PKU75_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPF81713.1"
                     /translation="MNLPTLPDTLREQHSGKGWPLALVGSLLLLVSGLLSWSFDPSIL
                     GDISIAFWPASLQLFAMILGAVGVLLALMHRGPLHRLGDWFDTALGLRALGTGSLLFV
                     VFSLVAIASEAGGLVNVNPGGWLALAGALLLTVGARFVPQRQLNSFAKAKANPVLEIV
                     VIVLGMAAILFGAAYVLGRDDPWIFVGFLVMAAVAFMALARAGFFTWMSHVAQRHRHV
                     LTLAAFVVAFLFPFTQDGSDANMSIATQVLIFAATAIGLNIVVGLAGLLDLGYIAFLG
                     SGAYVGAMMSNAASATIGWKPPFIVVMLIGACVAATLGLIIGSPTLRVSGDYLAIVTL
                     AFGEIFRLSMFNLDGNNGPDLTNGPNGIPGIPDLNLFGFDFGKPHTVFGIQLGRFSNY
                     YFILLLLIGFIILVFTRLNNSRIGRGWVAIREDERAAEAMGVNTFGLKLLGFAVGAFL
                     AGLAGTIKAHQDTAASPDQYIFLESAFLLAAVVLGGMGTVAGVLIGATILKLLPEKLR
                     FFSEYRLLLFGLLLVIMMRVRPEGLIASKRRQLEFHEDDEELAQEVQADHLIDAAPEA
                     AR"
     gene            complement(2178..3146)
                     /locus_tag="PKU75_12345"
     CDS             complement(2178..3146)
                     /locus_tag="PKU75_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPF81714.1"
                     /translation="MEIFLQQLVNGLTLGSLYALIAVGYTVVYGIVQLINFAHGEVFM
                     IGAFGALATYLLLFQGQTALWILPIMIIGAMIASVGTAVLMERVAYRPLRNAPRLAPL
                     ITAIGISVFLQEFVRLFFDKVPGTDFPSAKAKIPFPQIDVVTGSAFHPMEGVTIQRAA
                     IFTVAMLLLSAVGLWWFVNKTKLGRGMQAVSQDPDTARLMGINVDRIIVVAFAVGAAL
                     AAIAGVAQGLQITNVNFKMGFIAGLKAFTAAVLGGIGNIQGAVVGGLVLGLAEAFITQ
                     YTGMLPDPFGQFGGSSWKDVWAFALLIVILVFRPQGLLGAKVVDRA"
     gene            complement(3363..4601)
                     /locus_tag="PKU75_12350"
     CDS             complement(3363..4601)
                     /locus_tag="PKU75_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HPF81715.1"
                     /translation="MPKRSAVSVGATLLVASLALSACGSRGGSDTSSSAAGSGASAGG
                     ATKTAKIGVIAPLSGDLAALGLGIQNSVDLAIKQANETNAIPGWELELAAEDDQATAD
                     VGKNAATKLAGDKDVIGVVGTLNSSVALQVQPVLSSASIVQVSPANTGVSLTKGEDAA
                     NPARPFKTYFRTCTTDAIQGPFAARFLFESGIKEVATVHDKKAYGQGLVQAFTEEFEK
                     LGGTVVAAETINPDEDKYDAVVSAIKPKSPKALYYGGEYPNAGPLSQQMKSQGLNIPL
                     MGGDGIFDPKFIELAGSTSDGDLATSVGAPTDSTDSGKKFLEEYKAAGYSDPSAAYGG
                     YAYDAANSIINALKTSLANAASVEDARQATVDALGKVSFDGVTGKVAFDEFGDTTTKI
                     LTVYKVTGGEWKTEKTDEFK"
     gene            4817..5260
                     /locus_tag="PKU75_12355"
     CDS             4817..5260
                     /locus_tag="PKU75_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HPF81716.1"
                     /translation="MTHEIAESTRRSMLAASGASAAAVALTACGSGASDGTSSGSTGA
                     PGASGSAAAGGTRVAKADVPVGGGTILADAKVVVTQPSAGDYKAFSAVCTHQGCIVSS
                     VASTIDCACHNSVFSISDGSVVSGPATSALPAKTVTTSGDNLTIS"
     gene            5335..7314
                     /gene="uvrC"
                     /locus_tag="PKU75_12360"
     CDS             5335..7314
                     /gene="uvrC"
                     /locus_tag="PKU75_12360"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPF81717.1"
                     /translation="MADPSSYRPAPGSIPTDPGVYRFRDVHGRVIYVGKAKSLRSRLS
                     SYFQDIAALHPRTASMVRTGASVEWTVVTNEVEALQLEYSWIKEFDPRFNVKYRDDKS
                     YPYLAVTMGEQFPRAMVMRGAKRQGTRYFGPYAHAWAIRETLDLMLRVFPIRTCSAGV
                     FKRAGQVGRPCLLGYIDKCSAPCVGRVDEDRHREIAEDFCDFMAGDTGRFVKRLTAQM
                     KQAAAELEFEQAARLRDDIEAMTKALERSAVVFADATDADVFAIAEDGLEMAVQVFHV
                     RGGRVRGQRGWVAEMDRETVPDLVEQLLMQVYGGESADGIPREILLPVLPPEPLAMAE
                     LLRSVRGAKVAVKIPQRGDKKALLDTVRRNAEQALARHKVARAGDLTARAQALDELAD
                     ALGLDEAPLRIECFDVSHVQGTNVVASMVVFEDGLARKSEYRRFIVKGEQVDDTAAMR
                     EVLTRRFARYRIERTETEDGKAKKFAYPPNLLVVDGGRPQVDAAARVLEELGIVDIAC
                     VGLAKRLEEVWLPGSDFPVILPRGSEGLYLLQRVRDEAHRFAITFHRERRSKAMTTSA
                     LDGIPGLGAQRQKALRRHFGSVAKIRAATPEEIAVVPGFGSALAQAVWSHLQGSAAEP
                     AVNLTTGEILDEEGPGDGPADLDPADVERSDTMVP"
     gene            7341..8243
                     /gene="rapZ"
                     /locus_tag="PKU75_12365"
     CDS             7341..8243
                     /gene="rapZ"
                     /locus_tag="PKU75_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPF81718.1"
                     /translation="MTQPSADPDTDLDVEFLIVTGMSGAGRSTVGHVLEDLGWFVVDN
                     LPPSLLVDLVDLAHRVSRRPGRRHQRVAAIVDVRAREFSDSLSGAIDRLRQSGRTPTV
                     IFLDATDEALVRRFESVRRPHPLQGQGRLLDGIQAERQLLGELRSSASVLIDTSGLNV
                     HQLTRKITPLFSQIEQRTRLAVVSFGFKYGVPLDADFVFDMRFLPNPFWVPELRSLTG
                     TDAPVSEFVFSEPGAEEFVDRVAALMETVAAGYEREGRRYVTLAVGCTGGKHRSVAVA
                     EALGRRLEGDAFATFVVHRDLGRE"
     gene            8240..>8612
                     /locus_tag="PKU75_12370"
     CDS             8240..>8612
                     /locus_tag="PKU75_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HPF81719.1"
                     /translation="MTRVVALGGGHGLAASLSALRHVCDDITAIVTVADDGGSSGRLR
                     QEFGVLPPGDLRMALSALCAESDWGHQWRDVLQHRFSGDGPLGGHALGNLLILALWDL
                     DPEPVYGLDLVGRLLNARGRVL"
BASE COUNT         1432 a         2893 c         2950 g         1337 t
ORIGIN      
        1 tgccgagccc gacgaagtcg agcaattcct gcgcccgcga gcgggattcg gcctcctcgc
       61 ggcggtactt cggcccgtgg agcaccgacg tgatcgcgcc ggaggtggtg cggcagtagc
      121 gccccaccat gacgttctcg agtgccgtca tattgccgaa gagccggatg ttctggaacg
      181 tccgcgccat ccccgccttg accaccgcac ggggcttggg cggcaacacc tcgccgtaga
      241 acttcaccgt tcccgacgtc ggcttgtaca taccggtcag gcagttgaag aacgtcgtct
      301 tgccggcgcc gttgggcccg atcaggccga cgatctcgcc ctcgtggacg tcgaagtcga
      361 ccttgttcac agccaccaga ccgccgaagc gcatggtgac ctgctcggcg tccagcacct
      421 tggcaccgcg ttgggggcgg gctcggccgt cctggacctc ggtgtcgatc acggtcatcg
      481 ggctgcctcc ggtgcggcgt cgatcagatg gtcggcttgg acctcttggg cgagttcctc
      541 gtcgtcctcg tggaactcca gttgccgtcg tttgctggcg atcaggccct cggggcgcac
      601 ccgcatcatg atgacgagga ggagaccgaa gagcagcagc cggtactcgc tgaagaagcg
      661 cagcttctcg ggcagcagtt tcaggatggt ggcgccgatc aggacgccgg caacggtccc
      721 cataccgccg agcacgacgg cggcgagaag gaacgcggac tcgaggaaga tgtactggtc
      781 gggggaggcc gccgtgtcct ggtgcgcctt gatcgtcccg gcgagcccgg cgaggaaagc
      841 accgacggcg aagccgagca gcttcaggcc gaaggtgttg acccccatcg cctccgccgc
      901 acgctcatcc tcgcggatgg ccacccagcc gcgtccgatc cggctgttgt tgagccgggt
      961 gaagaccagg atgatgaacc cgatgagcag caacaggatg aagtagtagt tgctgaaccg
     1021 gccgagctgg atcccgaaga cggtatgcgg cttgccgaag tcgaagccga agaggttcag
     1081 atccgggatg ccgggtatgc cgttggggcc gttggtcagg tccggcccgt tgttgccgtc
     1141 caggttgaac atcgacagcc ggaagatctc accgaaggcc agggtcacga tcgcgaggta
     1201 gtcgcccgac acccgcaggg tcggtgagcc gatgatcaga ccgagagtgg ccgcgacgca
     1261 cgcaccgatc agcatgacga cgatgaacgg cggcttccag ccgatcgtgg ccgaggcggc
     1321 attcgacatc atcgcgccga catacgcccc ggagccgagg aaggcgatat agccgaggtc
     1381 gagaaggccg gcgagaccca cgacgatgtt gaggccgatc gcggtggccg caaagatgag
     1441 cacctgcgtg gcgatcgaca tgttcgcgtc cgagccgtcc tgggtgaacg ggaacaggaa
     1501 cgcgacgacg aaggcggcca gcgtcagcac gtgccggtgc cgttgcgcga catggctcat
     1561 ccaggtgaag aagccggcgc gggcgagggc catgaaggcg acggccgcca tgacgaggaa
     1621 gccgacgaag atccacgggt cgtctcgtcc caggacgtat gccgcaccga agaggatcgc
     1681 ggccatgccg agcacgatga cgacgatctc cagaacgggg ttcgccttgg ccttggcgaa
     1741 gctgttgagc tggcgctgcg gcacgaatcg tgccccgacc gtcagcagga gcgcgccggc
     1801 gagtgccagc cagccgccgg ggttgacgtt gaccaggcca cccgcctcgg aggcgatcgc
     1861 gaccagggag aacacgacga agagcagcga ccccgtgccg agggcgcgca gccccaaggc
     1921 ggtgtcgaac cagtcgccga gccggtgcag cggacccctg tgcatgagcg cgagcaggac
     1981 gccgacggcg ccgaggatca tcgcgaacag ctgcaggctg gcgggccaga aggcgatgga
     2041 gatgtcgccc aggatgctgg ggtcgaaact ccaggagagc aggccgctga ccagcagcag
     2101 cagggaaccc acgagcgcca gcggccagcc tttgccggag tgctgctcgc ggagggtgtc
     2161 ggggagggtc ggcaggttca cgcgcggtcc accaccttcg cgccgagcag gccttggggc
     2221 cggaatacca ggatgacgat gagcaacgcg aaggcccaca cgtccttcca ggaggacccg
     2281 ccgaactgtc cgaagggatc gggcagcatg ccggtgtact gggtgatgaa ggcctcggcg
     2341 agcccgagca ccaagccgcc cacgacggcg ccctggatat tgccgatgcc gcccaggacg
     2401 gcagcggtga acgccttcag gccggcgatg aagcccatct tgaagttgac attcgtgatc
     2461 tgcaggccct gtgccacgcc cgcgatcgcc gccagcgcgg cacccacggc gaaggcgacg
     2521 acgatgatgc ggtcgacgtt gatgcccatc aggcgtgcgg tatcggggtc ttgcgagacc
     2581 gcctgcatgc cgcggcccag cttggtcttg ttgacgaacc accacaggcc cacggcactc
     2641 agcaggagca tcgccacggt gaagatcgcc gcccgctgaa tcgtgacgcc ctccatcggg
     2701 tggaaggcac tcccggtgac gacgtcgatc tgcgggaagg ggatcttggc cttcgcggac
     2761 gggaagtcgg tgccgggcac cttgtcgaag aacaagcgca cgaactcctg gagaaacacc
     2821 gagatgccga tggcggtgat caggggtgcc aggcgagggg cattgcgcaa gggccggtag
     2881 gccacccgct ccatgagcac ggcggtcccc accgacgcga tcatcgcccc gatgatcatg
     2941 atcggcagga tccacagcgc ggtctggcct tggaagagga gcagataggt ggccagggct
     3001 ccgaacgcgc cgatcatgaa cacctcgccg tgggcgaagt tgatcaactg gacgatgccg
     3061 tagacgacgg tgtagcccac ggcgatcagc gcgtagaggg agcccagcgt cagcccgttg
     3121 acgagctgct gcaggaagat ctccacgaag ggtctgctcc ttaggtgtca ggccgcgcgc
     3181 cgggggtgac cggcgtcaca gcgcgagtgc tgggagaaag ctagcgaacg gggccgacat
     3241 cgagataacg cgacgcctcg agaggagcgc accgcattgc gcgcactccg aatgcaatgg
     3301 cgtcgatgac ggacagcgtg ctgcccccca tcgacgccat tgcctcggat cggcctagcc
     3361 gatcacttga attcgtcggt cttctcggtc ttccactctc cgccggtcac cttgtagacc
     3421 gtgaggatct tggtggtggt gtcgccgaac tcgtcgaagg cgaccttgcc cgtgacgccg
     3481 tcgaagctga ccttgccgag ggcgtcgacg gtggcctggc gggcatcctc gacgctggcg
     3541 gcgttcgcca gcgaggtctt cagcgcgttg atgatggagt tcgccgcgtc atacgcatag
     3601 ccgccgtagg ccgcagacgg gtccgagtag ccggctgcct tgtactcctc caggaacttc
     3661 ttgccggagt cggtgctgtc ggtcggggcg cccaccgacg tcgcgaggtc gccatcggac
     3721 gtcgacccgg cgagttcgat gaacttcggg tcgaagatgc cgtcaccgcc catgagcggg
     3781 atgttcaggc cctggctctt catctgctgg ctcagcgggc ccgcgttggg gtactcgccg
     3841 ccgtagtaga gggccttggg gctcttcggc ttgatcgccg agacgacagc gtcgtacttg
     3901 tcctcgtccg ggttgatcgt ctcggccgcg acgacggtgc cgccgagctt ctcgaactct
     3961 tcggtgaagg cttggaccag gccctgaccg taggccttct tgtcgtggac ggtcgcgacc
     4021 tccttgatgc ccgactcgaa gaggaagcgg gcggcgaagg ggccctggat ggcgtcggtg
     4081 gtgcaggtgc ggaagtaggt cttgaacggc cgggccgggt tcgccgcgtc ctcgcccttg
     4141 gtcagggaga caccggtgtt ggcgggggag acctggacga tactcgccga gctgaggacg
     4201 ggctggacct ggagggcaac gctggagttc agggtgccga cgacgccgat gacgtccttg
     4261 tcgcccgcca gcttggtggc ggcgttcttg ccgacatcgg ccgtggcctg gtcgtcctcg
     4321 gccgccaact cgagctccca gcccgggatg gcgttggtct cgttggcctg cttgatggcc
     4381 aggtcgacgg agttctggat gccgaggccc agcgctgcca ggtctccgga cagcggggcg
     4441 atgacgccga tcttggcggt cttggtggca ccgccggcgc tggcaccgga tccggctgcg
     4501 gacgatgagg tgtcgctgcc gccgcgggat ccgcacgcgg acagggcaag cgaggcgacg
     4561 agcaaggttg cgccgacgga gacggcggag cgcttgggca cgagtgttcc tcctgtatta
     4621 cgtgccgacg gttcacgtcc cgtcggaccc ggcggttggc cggagtaggc gcacagtatc
     4681 cgcaaatcgg taaggagcaa cacgcctcgc cggttgtgta gtcgtctcgt taccgcagga
     4741 cccgactttt aacgctcaag aaacatctcg ctcgcctagg gtgggcgccc gtaacgactc
     4801 aacgagaggt atgccgatga cgcacgaaat cgccgaatcc acgcgtcgat ccatgctcgc
     4861 cgcgagcgga gcgagtgccg ccgcggtggc gctgacggcc tgcggatccg gggcgagcga
     4921 tgggacatcg agcgggagca ccggagcgcc cggcgcatcc gggtcggccg cggccggcgg
     4981 cacgcgtgtc gcgaaggcgg atgtccccgt cggtggcggc accatccttg ccgacgccaa
     5041 ggtcgtcgtc acccagccga gcgcggggga ctacaaggcg ttcagcgcgg tctgcaccca
     5101 ccaaggctgc atcgtcagtt cggtcgcgag cacgatcgac tgtgcctgtc acaacagcgt
     5161 cttctccatc agcgacgggt ccgtcgtgtc cggcccggcg acgtccgcgt tacccgcgaa
     5221 gaccgtcacc acgagtggcg acaacctcac catcagctag agcgttagat ccattgttca
     5281 tggcctgacc tgcgcgaatc caccgctgtc ggcccccgag cctaagctgc acgggtggcc
     5341 gatccctcgt cataccgccc tgccccgggg tcgatcccga cggatcccgg ggtctatcgg
     5401 ttccgggatg tccacggccg cgtcatctat gtcggcaagg ccaagtcgct gcgctcgcgg
     5461 ctctcgagct acttccaaga catcgccgcc ctgcatccgc gcacggcatc catggtgcgc
     5521 accggcgcct ccgtcgagtg gaccgtcgtc accaacgagg tggaggcgct ccaactcgaa
     5581 tactcctgga tcaaggagtt cgacccgcgg ttcaacgtca aatatcgcga cgacaagtcc
     5641 tatccctatc tcgccgtgac gatgggggag cagttccccc gcgcgatggt gatgcgcggc
     5701 gccaagcggc agggcacccg atacttcggg ccctatgccc acgcgtgggc gatccgcgag
     5761 accctcgacc tgatgttgcg ggtcttcccg atccgcacct gcagcgccgg cgtcttcaaa
     5821 cgcgccggcc aggtgggccg gccctgcctg ctcggctata tcgacaagtg cagcgccccg
     5881 tgcgtgggcc gggtcgacga ggaccggcac cgcgagatcg ccgaggactt ctgcgacttc
     5941 atggccggcg acaccggccg cttcgtcaag cgcctcacgg cccagatgaa acaggccgcc
     6001 gccgagctgg agttcgagca ggccgcccgg ctgcgcgacg acatcgaggc gatgaccaag
     6061 gcgctcgagc gctcggccgt ggtcttcgcc gacgccaccg acgccgacgt cttcgccatc
     6121 gccgaggacg gcctggagat ggcggtccag gtcttccacg tccgcggcgg gcgagtgcgc
     6181 ggccagcgcg gctgggtcgc cgagatggac cgcgaaaccg tgcccgacct cgtcgagcag
     6241 ctgctcatgc aggtctacgg cggggagtcc gccgacggca taccccgcga gatcctgttg
     6301 ccggtgctgc ctccggaacc gctggccatg gccgagctcc tgcgctcggt gcgcggcgcc
     6361 aaggtcgccg tcaagatccc gcaacgcggc gacaagaagg ccctcctcga caccgtgcgc
     6421 cgcaacgccg agcaagccct cgcccgacac aaggtcgccc gcgccggcga cctgaccgcc
     6481 cgcgcccagg ccctcgacga actcgccgac gccctcggcc tcgacgaggc cccgctgcgg
     6541 atcgagtgct tcgacgtctc ccacgtccag ggcaccaatg tcgtggcctc gatggtcgtc
     6601 ttcgaggacg ggctggcccg caaatccgaa taccgccggt tcatcgtcaa gggcgaacag
     6661 gtcgacgaca ccgccgcgat gcgcgaggtc ctcacgcggc gcttcgcccg ctaccgcatc
     6721 gaacgcaccg agaccgagga cggcaaggcc aagaagttcg cctacccgcc caaccttctc
     6781 gtcgtcgacg gcggccgtcc gcaggtcgac gcggccgctc gcgttctcga agagctgggg
     6841 atcgtcgaca tcgcctgcgt gggtctcgcc aagcgactgg aggaggtatg gctgccgggc
     6901 agcgacttcc cggtgatcct cccgcgcggc agcgagggtc tgtatctgct gcaacgggtt
     6961 cgcgacgaag cgcaccgctt cgccattacc ttccatcgcg aacggcgcag caaggcgatg
     7021 acgacgagcg cactcgacgg catacccggc ctcggcgcgc agcgccagaa agccctgcgc
     7081 cggcatttcg gctcggtcgc gaagatccgc gccgccaccc ccgaggagat cgccgtcgtg
     7141 cccggcttcg ggtccgccct cgcccaggcc gtatggagcc acctgcaggg cagcgccgcc
     7201 gaacccgccg tcaacctcac gacgggcgag atcctcgacg aagaaggccc tggggatggg
     7261 ccggcggatc tcgacccggc agacgtcgaa cgatccgaca caatggtccc gtgagcgagc
     7321 cggccgctga ggtggacccg atgacgcagc cctcggctga ccccgacacc gatctcgacg
     7381 tcgaattcct catcgtcacc ggcatgagtg gcgccggtcg ctcgacggtg ggccacgtcc
     7441 tcgaggacct cggctggttc gtcgtcgaca acctcccccc atcgctgctc gtcgacctcg
     7501 tcgacctcgc ccaccgcgtc tcgcggcgcc ccggccggcg gcatcagcga gtggccgcga
     7561 tcgtcgacgt gcgcgcgaga gagttctccg actcgctgag cggcgcgatc gaccggctac
     7621 gccagagcgg gcgtaccccg accgtgatct tcctcgacgc caccgacgag gcactggtgc
     7681 ggcgcttcga gagcgtccgg cggccgcacc cgctgcaggg acaagggcgg ctgctcgacg
     7741 gcatacaggc ggagcggcag ctcctcggcg aactgcgtag cagcgcaagc gttctgatcg
     7801 acaccagcgg actcaacgtc caccagctga cccgcaagat caccccgctc ttctcgcaga
     7861 tcgagcagcg cacccggctg gcggtcgtct cgttcgggtt caagtacggc gtgcccctcg
     7921 atgccgactt cgtcttcgac atgcgcttcc tgcccaaccc cttctgggtg ccggagctgc
     7981 gcagcctcac cggcaccgac gccccggtct cggagttcgt cttctccgag ccgggcgcgg
     8041 aggagttcgt cgatcgggtc gcggcgctca tggagaccgt cgcggccggg tatgaacgcg
     8101 aaggtcggcg ctatgtcacc ctcgccgtcg gctgcaccgg cggcaagcac cggtccgttg
     8161 ccgtggccga ggcgctcggg cggcgactgg agggtgacgc cttcgccacc ttcgtcgtcc
     8221 atcgtgacct ggggcgcgaa tgacgcgcgt cgtcgccctc ggaggagggc acgggctggc
     8281 cgcctcgctc tcggcgttgc ggcatgtctg cgacgacatc accgcgatcg tcaccgtcgc
     8341 cgacgacggc ggctccagcg gccggctgcg ccaggagttc ggcgtcctgc cgccggggga
     8401 cctccgcatg gcgctgtcgg ccctgtgcgc cgagagcgac tggggtcacc agtggcggga
     8461 tgtgctgcag caccgcttct cgggggacgg cccgctcggc ggccacgccc tgggcaatct
     8521 gctcatcctc gcgttgtggg atctcgatcc ggagccggtc tacggtctcg acctcgtcgg
     8581 gcggctgctc aatgcccgcg gccgggtgct gc
//
LOCUS       DAOPJG010000147         8532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8783_length_8532_cov_8.381857, whole genome shotgun sequence.
ACCESSION   DAOPJG010000147 DAOPJG010000000
VERSION     DAOPJG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8783_length_8532_cov_8.381857"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PKU75_12375"
     CDS             complement(<1..843)
                     /locus_tag="PKU75_12375"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HPF81720.1"
                     /translation="MRTKGTAAIVGPGNIGTDLMFKLMRRSDIIEPRYMIGVDPQSDG
                     LRRAKSVGLEASAEGVDWLLAQEELPDIVFECTSAKAHEANAPRYAEAGILAVDLTPA
                     AVGPYLCPPVNLEFNLDAMNVNMITCGGQATTPMVKAVSSVVPVPYAEIVASISSLSA
                     GPGTRANIDEFTETTSAALEVVGGAERGKAIIILNPVEPPLMMRNSVFCAIPEEAGEP
                     GELQDKILESIHAMVRTVQDYVPGYHLRAEPQFDHARETWQGMARVFVPIEVKGRGDY
                     LPDYA"
     gene            complement(857..1636)
                     /locus_tag="PKU75_12380"
     CDS             complement(857..1636)
                     /locus_tag="PKU75_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HPF81721.1"
                     /translation="MDDNTRAHAAERLLAAYDGGPIPPLTATYEGMALDDAYAVQQLQ
                     ISALLERGRTIKGHKVGLTSAAMQKLLGVSEPDYGHLLDDFFYLEHMPIPSLRFLQAR
                     IEPEVAFVLKKPLQGPGVTVSEAISAVDYVLPALEIVDSRIENWKIGLFDTIADNASS
                     AAVVLGSTPTPLADVDLRLMGAVMHKNGQVAGTGAGGAVLGSPINSLVWLANTVGARG
                     VVLDAGHVILPGAVCAMVPVAAGDTVTATFAGLGSVTARFS"
     gene            complement(1808..2617)
                     /locus_tag="PKU75_12385"
     CDS             complement(1808..2617)
                     /locus_tag="PKU75_12385"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPF81722.1"
                     /translation="MSTLTDDSAWVLTTPKRIRPTDSAAASLKSVGSALDVLECFATD
                     GELGVSDIARRLGCAKSTAHRLLSTLVSRGFVEQDVETGQYRLGIHIYELGQLALQRN
                     QLRYVALPIMRQVAQSTGLTVNLAVPDGADVVFVERLENTDGVRILGHAGRRLPAHCT
                     SSGKIIAAFNDRANAARLAAGFPPRARGTVRSERDWIRVIEQARKDGYAASTSESFDG
                     ASSVAVPIMRGKQAVGSLSAFGPTEAVTPRIDKLVPFLTAAANRIAKSYSG"
     gene            2681..3466
                     /locus_tag="PKU75_12390"
     CDS             2681..3466
                     /locus_tag="PKU75_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPF81723.1"
                     /translation="MRSTDRNTARDARETGNRTVLHRILAVLGAFDTSTTRLGLAELC
                     RRAGLPKTTVHRIAGELVEERLLVRDEQGLYALGMRLFEIGELVPVHRTLSSVALPIM
                     EDLREATRKRVHLAVLDGIDVVYVEILGATSIDVGSRPGGRIPAHATGVGKVILAYSP
                     TATIQARLEAGLPALTPRTITTKDALVADLRKIRSIGMSLDLEESHLGVSCVAAPVFS
                     FNKRIRAGLSITGPIGSMDPGLLGPAVRTAAFTLSRALRDAGL"
     gene            3558..5261
                     /locus_tag="PKU75_12395"
     CDS             3558..5261
                     /locus_tag="PKU75_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPF81724.1"
                     /translation="MGGRHARRSPVPAYAAAAALAVGLTATVGILAMRDDESPASASG
                     TNSTQSRSATGSATTPANTTGGAECAPDAAPLHVLVAPELDAVTQAAAERVTASDPCR
                     TYTVDTAADREGVTAAQADQPPQVWVPDSVTWLDALSSQARDRYRVDAGVAQSPIALA
                     RPAGADGAAPASWADVVRGSEPLKLADPDRDAASRLAFYTAWAQRSGPLDRAAGSRLV
                     LLSRYTKNSTSALLKDYETKPDDASAFPISERAAYAFDRQRPKEPLVLAFPRTGTPVL
                     DYPWLSLKQLSAAQARDMEALRAELDSSESRDALTAAGFRVRGADDAGPKVAGRSARP
                     TIIDNPESATREEATAQWDLLRTDMRMLAVIDVSGSMAWDSGTPGQSRMDVLLGSAGR
                     ALGTLAAGSEIGAWVFSTAQDGPKQDWRQLVTIRRLDAKVNGHTQRDELRRQLARIPS
                     RIQGDTGLYDTTLAAFLRMRDSYDGNFVNSVVLMTDGVNDDTTGGLGLRTLLSRLKDE
                     FDPDRPVRVVTIGMGEADASALKQISAATGGTSYIANRAEDIDRVLVQALLARPLPVA
                     K"
     gene            complement(5274..7685)
                     /locus_tag="PKU75_12400"
     CDS             complement(5274..7685)
                     /locus_tag="PKU75_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HPF81725.1"
                     /translation="MSSTVTPRTPDVDVDELDAYHRRHDRRWWIALACVLIGSFGVLL
                     WMGKAINTHKPPIPAAVTVTGGSQLLTREDIVEGQKIWQSLGGQEIGSVWGHGAYVAP
                     DWTADWLHREATFILDRWASEEGAATYAALDPEPQGALKARLKELLRTNTFDAASQTI
                     TLDPIRQEAFDANAAYYAKLFADGNHEYAIPKGVLTDAADARRMASFFWWTSWASSTN
                     SPDADITYTQNWPHEPLIDNVPPPSNVLWSIISIILLLAGIAGMVWYHTANDNRDREA
                     DEHAAAPHDDPLLGYQSTPSQRATLKYFFIVGLLFVLQIAMGIIAAHYAVEGGALYGI
                     PIDQVLPYAVVRTWHTQLGIFWIATAWLATGLYVAPAVGGREPKFQRLGVNVLCGALV
                     VVVLGSMAGEWASIMGKIGRGDVANFWFGTQGMEYVDLGRIWQIGLTIGLFLWLFLMW
                     RAASPALKRSSEINKLMATSDAPLAAGSQRSLVAMLLVSCLAIAGFYGAAFGIGHETH
                     LSIAEYWRWWVVHLWVEGFFEVFATVVMAFLFSRLGLIRPRTATSATLAATVIFLAGG
                     IIGTAHHLYFTGSPTPVIALGASFSALEVVPLSLMGFEAFRHLRLLKVRKWVAGYKWA
                     IYFFVSVAFWNMLGAGVFGFLINPPISLFYVQGLNLTPLHGHTALFGVYGMLGIGLML
                     FCVRSLMPGREWNDKPIAIGFWCMNIGLFLMAFMSLLPVGLGQAWASINEGLWYARSS
                     EFLSGDTMTLLVWLRVPGDTIFGIGALAIGVFMVGLLTGWSLKKDGGAVEQGSVTAVD
                     ESVDA"
     gene            7767..8531
                     /locus_tag="PKU75_12405"
     CDS             7767..8531
                     /locus_tag="PKU75_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF81726.1"
                     /translation="MGTKRPASDAAAAAAAAQASATRRALGEPTRFAIVQYLHTADRA
                     ARVDELAQAMSVHHNAIRAHLNVLMEVGIVERERAAGLARGRPAWVHRLTPEASAVWR
                     GSAPQEELALMLLDVLTTKRSPREVGRERGAELAAAVADELVADQPGDAGPGDAGPGD
                     AVSGDAGPGGRARRVLEVITRDLGFAPRTDPAAPHVILLRHCPFAAAASQAREIICGL
                     HLGVAEGVCRATGDALSVAALHVADPHVGPCRLELA"
BASE COUNT         1375 a         2881 c         3002 g         1274 t
ORIGIN      
        1 ggcgtagtcg ggcagataat cgccgcggcc cttgacctcg atggggacga agactcgggc
       61 catgccctgc caggtctcgc gtgcgtggtc gaactgcggt tcggccctca ggtgatagcc
      121 ggggacgtag tcctgcacgg tgcggaccat cgcgtggatg gactcgagga tcttgtcctg
      181 gagctcaccg ggctcgcccg cttcttcggg gatggcgcag aacacggagt tgcgcatcat
      241 cagcggcggt tcgacggggt tgaggatgat gatcgccttg ccgcgctccg cgccgccgac
      301 gacttccagg gcggcggagg tggtttcggt gaactcgtcg atattggccc gagtgcccgg
      361 cccggccgac aagctcgaga tcgacgcgac gatctcggca tacggcacgg ggacgacgga
      421 gctgacagcc ttgaccatcg gggtcgtggc ttgcccgccg caggtgatca tgttgacgtt
      481 catcgcgtcg aggttgaact cgaggttgac cggcgggcag aggtaaggtc cgacggctgc
      541 gggggtgagg tcgacggcga ggatgccggc ctcggcatag cgtggcgcgt tggcctcgtg
      601 cgccttggcc gacgtgcact cgaagacgat gtcgggcagc tcctcctgcg cgagcagcca
      661 gtcgacgccc tcggcgctgg cttccagccc aacggatttc gcgcgacgca gcccgtcgct
      721 ctgggggtca acgccgatca tgtagcgcgg ttcgatgatg tcgctgcggc gcatcagctt
      781 gaacatcagg tcggtgccga tattgcccgg cccgacgatg gcggcagtgc ctttggtacg
      841 cacgtgaatc tcctagttat gaaaaccgag cagtgacgga gccgaggccg gcgaaggtcg
      901 ctgtgaccgt gtcaccggcg gcgacaggaa ccatggcgca gaccgcgccg ggcaggatga
      961 cgtggcccgc gtcgaggacg actccgcgtg cgccgaccgt attggcaagc cagacaaggg
     1021 aattgatggg cgagccgagg actgctccgc cggcgcccgt gccggcgacc tggccgttct
     1081 tgtgcatgac ggcacccatc aggcgcaggt cgacgtcggc aagcggcgtc ggcgtcgagc
     1141 cgaggacgac cgcggcggag gaggcgttgt cggcgatggt gtcgaagaga ccgatcttcc
     1201 agttctcgat gcgggagtcg acgatctcca gtgcgggcaa cacatagtcg acggccgaga
     1261 ttgcctccga gacagtgact cccggcccct gcagcggctt cttgagcacg aaagcgacct
     1321 cgggctcgat gcgtgcctgg aggaagcgta aactcgggat aggcatgtgc tcgagataga
     1381 agaagtcgtc gagcaggtgc ccgtagtcgg gctcgctcac cccgagcagt ttctgcatgg
     1441 cggccgaggt cagccccacc ttgtgcccct tgatcgtgcg gccgcgctcc aggagggccg
     1501 agatctgcag ctgctgcacg gcgtacgcat cgtcgagggc catgccctcg taggtcgcgg
     1561 tgagcggtgg gatgggaccg ccgtcgtatg ccgcgagcag gcgctcggcg gcgtgggcgc
     1621 gggtgttgtc gtccacgctt ctcctttcgc cggagacgga tgtctccagt tgtgacgcta
     1681 tgagtgcagg acagggggca gggtgctcgt tccgcctgcg ggaacgagcc gcttcggtgc
     1741 tggcgacgag tagggtgtct tcgtcgtgtc cccgcgaagg agcagagcgg agaacgtcgg
     1801 agctgcctta tcccgagtag gacttggcga tgcgattggc ggcggcggtg aggaagggca
     1861 cgagcttgtc gatccggggg gtgaccgcct ccgtcgggcc gaacgcggac aaggacccga
     1921 ccgcctgctt gccccgcatg atcgggacag ccaccgagga ggcgccgtcg aacgactcgc
     1981 tggtggacgc ggcatacccg tccttgcgag cctgctcgat gacgcggatc cagtcccgct
     2041 ccgagcggac ggtgccgcgc gctcgtggcg ggaagccggc ggccagccgg gcggcgttcg
     2101 cgcgatcgtt gaaggccgcg atgatcttgc ccgacgaggt gcagtgtgcc ggcaggcgcc
     2161 gcccggcatg gccgaggatc cgcacgccgt cggtgttctc cagccgctcg acgaagacga
     2221 cgtcggcgcc gtccggcacc gctaggttga cggtcaggcc cgtcgactgg gcgacctgcc
     2281 gcatgatcgg cagcgcgaca taccgaagtt ggttgcgctg caacgccagt tggcccagct
     2341 cgtagatgtg gatgccgagg cggtactgcc cggtctccac gtcctgctcg acaaacccgc
     2401 ggctgacgag agtcgagagc aagcggtgcg cggtggactt ggcgcagccg aggcgacggg
     2461 cgatgtccga cacacccagc tcgccgtcgg tggcgaagca ttcgaggacg tcgagcgccg
     2521 agccgaccga cttcagactc gccgcggcgg agtcggtggg acggatccgc ttgggcgtcg
     2581 tcagaaccca ggcactgtca tcggtcagcg tgctcaccgc catccctcct cgtcgagtcg
     2641 atgggatgac aatcccggca aactccgatt gaatcaaggg atgcgttcca ctgaccggaa
     2701 caccgcccgc gacgcccgcg agacggggaa ccggaccgtg ctgcaccgca tactcgccgt
     2761 gctcggcgcc ttcgacacga gcaccacccg gctcgggctg gcggagttgt gccggcgcgc
     2821 cgggctgccc aagacgacgg tgcaccggat cgcgggcgag ttggtggagg agcggctgct
     2881 ggtgcgtgac gagcagggac tgtatgcgct gggcatgcgg cttttcgaga tcggtgaact
     2941 cgtcccggtg caccggacgc tgtcgtcggt cgccctgccg atcatggagg acctgcgcga
     3001 ggccacccgc aaacgcgtgc atctggccgt gctcgacggg atcgacgtgg tctacgtcga
     3061 gatcctcggg gccacgtcga tcgacgtcgg ctcgcgaccg ggcggccgga tcccggctca
     3121 cgccaccggg gtggggaaag tcatcctcgc gtactccccc acggcgacga tccaggcgcg
     3181 cctcgaagcc ggcctgcccg ccctgacccc gcgaaccatc accaccaagg acgcgctcgt
     3241 cgccgacctg cgcaagatcc gcagcatcgg catgtccctc gacctcgagg aatcccacct
     3301 cggcgtctcc tgcgtcgcgg ctccggtctt cagcttcaac aagcgcatcc gcgccgggtt
     3361 gtcgatcacc ggcccgatcg gctcgatgga ccccggcctc ctcggcccgg ccgtccgcac
     3421 cgccgccttc acgctgtccc gggcgcttcg ggacgccggt ctctaggccc cgaactggtc
     3481 acgccgcgac gtgccctccg ccggcgggac acggccccgg cgcgcggccg gtgcctcggc
     3541 ccgtatggtt ttccctcatg ggtggacggc acgcacgacg cagcccggtc cctgcgtatg
     3601 ccgcggccgc ggcgctcgcg gtcgggctga cggcgaccgt cggcatcctc gcgatgcggg
     3661 atgacgaatc accggcatcc gcgtcgggaa cgaattcgac gcagtcacgc agcgccaccg
     3721 gctcggcgac aaccccggcg aacacgaccg gcggtgccga gtgcgccccc gacgccgccc
     3781 cgttgcacgt gctcgtcgcg cccgaactcg acgcggtcac gcaggcggcg gccgagcgcg
     3841 taaccgcatc cgacccgtgc cggacctaca ccgtcgacac cgccgcggac cgagagggag
     3901 tgacggctgc gcaggccgac cagcccccgc aggtctgggt gccggactcg gtcacctggc
     3961 tcgacgcgtt gtcctcgcag gccagggatc gctaccgcgt cgacgccggc gtggcgcagt
     4021 cgccgatcgc cctcgcccgg ccggcaggcg ccgacggcgc ggctcccgcc tcgtgggcgg
     4081 atgtcgttcg ggggtctgag ccgttgaagc tcgccgatcc ggaccgcgac gccgcctccc
     4141 gcctggcgtt ctatacggcc tgggcacaac gatccggccc cctcgatcgg gcggccggaa
     4201 gccggctggt gctcttgtcc cggtacacca agaactcgac gtcggccttg ctcaaggact
     4261 acgagaccaa acccgacgac gcctccgcct tcccgatctc ggagcgggcg gcatacgcgt
     4321 tcgatcggca gcggcccaag gagccactcg tccttgcttt cccgaggacg ggcacgccgg
     4381 tcctcgacta tccgtggctc agcctgaagc agttgtcggc ggctcaggcc cgcgacatgg
     4441 aagcgctgcg cgccgagctc gactccagcg aaagccggga tgccttgaca gcagccggtt
     4501 ttcgcgtgcg gggagcggac gacgccggcc cgaaggtcgc gggccggtcg gcccggccga
     4561 cgatcatcga caacccggag agcgccacgc gggaggaggc aacggctcag tgggacctgt
     4621 tgcggacgga catgcgcatg ctcgcggtga tcgacgtttc cggatcgatg gcgtgggaca
     4681 gcggcacccc tggacagagc cgtatggatg tcctcctcgg ctcggccggc cgggccctgg
     4741 gcactcttgc cgctggcagc gagatcggcg cctgggtctt ctcgaccgcg caggacgggc
     4801 cgaagcagga ctggcgtcaa ctcgtcacca tccggcgcct cgacgccaag gtcaacggcc
     4861 atacccagcg cgacgaactg cgccgccagc tggcacggat cccgagccgc atacagggag
     4921 acaccgggct ctacgacacg actctggccg cgttcttgag aatgcgcgat tcttacgacg
     4981 gcaacttcgt caactcggtc gtgctgatga ccgacggcgt caatgacgac accacaggcg
     5041 ggctcggcct ccgaacgttg ctgagccggc tgaaggacga attcgacccg gaccgcccgg
     5101 tgcgcgtggt cacgatcggc atgggcgagg ccgatgcctc cgcgctgaag cagatctcgg
     5161 cggccacggg cggcacctcc tatatcgcca accgggcgga ggacatcgac cgggtcctgg
     5221 tgcaggcgct cctggcccgc ccgctgccgg tcgcgaagta ggccggacgc gggtcaggcg
     5281 tcgaccgact cgtcgacggc cgtcacgctg ccctgctcca ccgccccgcc gtccttcttc
     5341 aacgaccagc ccgtcaacag gccgaccatg aagacgccga tggcgagtgc gccgatgccg
     5401 aagatcgtgt cgcccggcac ccgcagccac accaggaggg tcatggtgtc gccggagagg
     5461 aactcgctgc tgcgggcata ccacaagccc tcgttgatgc tcgcccaggc ctgacccagc
     5521 ccgaccggga gcagggacat gaacgccatg aggaagaggc cgatgttcat gcaccagaag
     5581 ccgatcgcga tgggcttgtc gttccactcc cggcccggca tcaggctgcg gacgcagaag
     5641 agcatcagcc cgatcccgag cattccgtag acgccgaaga gcgccgtatg cccgtgcaac
     5701 ggagtgagat tcaggccctg gacatagaac aaggagatcg gcgggttgat gaggaagccg
     5761 aagacgccgg ctcccagcat gttccagaag gcgaccgaga cgaagaagta gatcgcccac
     5821 ttgtagcccg cgacccactt gcggaccttg agcagccgca agtgccggaa ggcctcgaaa
     5881 cccatcagcg agagcggcac cacctcgagt gcggagaagc tcgcgccgag agcgatgacc
     5941 ggggtcggcg agccggtgaa gtagaggtgg tgagccgtgc cgatgatgcc gccggcgagg
     6001 aagatcaccg tcgcggccag ggtggcgctc gtcgccgtgc gcgggcggat gagacccagc
     6061 cgcgagaaga ggaaggccat gaccaccgtc gcgaagacct cgaagaagcc ctccacccac
     6121 aggtgcacga cccaccaacg ccagtactcg gcgatggaca gatgggtctc gtgcccgatc
     6181 ccgaaggcgg cgccgtagaa accggcgatc gccaggcagg agacgagcag catcgcgacg
     6241 agcgagcgct gcgatcccgc ggcgagcggt gcgtccgagg tggccatgag cttgttgatc
     6301 tcgctcgagc gcttcagcgc cggggatgcg gcgcgccaca tgaggaagag ccagaggaac
     6361 aggccgatgg tcagcccgat ctgccagatc cggcccaggt cgacgtactc cataccctgc
     6421 gtcccgaacc agaagttcgc cacgtccccg cggccgatct tgcccatgat cgaggcccac
     6481 tcgccggcca tcgacccgag cacgacgacg acgagcgcgc cgcacagcac gttgacgccg
     6541 aggcgctgga acttcggttc gcgcccgccg acggcggggg cgacgtacag acccgtggcc
     6601 agccacgcgg tcgcgatcca gaagatgccg agctgcgtat gccaggtgcg caccacggca
     6661 tacggcagca cctggtcgat gggtatgccg tacagcgcgc cgccctcgac tgcgtagtgg
     6721 gcggcgatga tgcccatggc gatctggagg acgaagagca ggccgacgat gaagaagtac
     6781 ttcagcgtcg cccgctgcga gggggtggac tggtagccga gcagcgggtc gtcgtggggc
     6841 gcggcggcgt gttcgtcggc ctcgcggtcg cggttgtcgt tggcggtgtg ataccagacc
     6901 atgccggcga tgccggcgag cagcaggatg atgctgatga tgctccacag cacattgctc
     6961 gggggcggga cattgtcgat cagcggctcg tgcggccagt tctgggtgta ggtgatgtcg
     7021 gcgtccggcg agttcgtcga cgacgcccac gacgtccacc agaagaagga cgccatccgg
     7081 cgcgcgtccg cggcgtcggt gagcacaccc ttggggatgg cgtattcgtg gttgccgtcg
     7141 gcgaagagct tggcgtagta cgcggcattg gcgtcgaacg cctcctggcg gatcggatcc
     7201 agggtgatcg tctggctcgc ggcgtcaaaa gtgttggtgc gcaacagctc cttcaggcgc
     7261 gccttgagcg ccccctgcgg ctcggggtcg agagcggcat acgtcgcggc gccctcttca
     7321 ctcgcccacc ggtcgaggat gaacgtcgcc tcgcgatgca gccagtcggc ggtccagtcg
     7381 ggcgcgacgt aggcgccgtg gccccacacc gagccgatct cctgtccacc gagggactgc
     7441 cagatcttct gcccctcgac gatgtcctcg cgggtgagca gctggctccc gcccgtgacg
     7501 gtcacggccg cggggatggg cggcttgtgc gtgttgatcg ccttgcccat ccacagcaac
     7561 actccgaagg agccgatcag gacgcaggcg agggcgatcc accaacggcg gtcgtgccgg
     7621 cggtggtagg cgtccaactc gtcgacgtcg acgtccggcg tgcgaggcgt gaccgtgctg
     7681 ctcatgaaaa ccctcctggc ggtgaggctt tttccactcg acaacggaaa tagtgccagg
     7741 tcacggccta ggtccacaat ggccccatgg gcaccaaacg ccccgcctcc gacgcggcgg
     7801 ccgcagcggc cgccgcccag gcgagcgcaa cgcgtcgggc tctgggcgag ccgacgcggt
     7861 tcgcgatcgt ccagtacctg cataccgccg accgggccgc gcgcgtcgac gagctggcgc
     7921 aggcaatgtc agtgcaccac aacgcgatcc gcgctcatct caacgtgctc atggaggtcg
     7981 gcatcgtcga gcgggagcgg gcggccgggc tcgcgcgcgg ccggccggcc tgggtgcacc
     8041 gcctgacccc ggaggcgagc gccgtatggc gtggctcggc cccgcaggaa gagctcgcct
     8101 tgatgttgct cgatgtgctg acgaccaagc gctccccgcg ggaggtcggg cgcgagcgcg
     8161 gtgccgagct ggcggccgcc gtcgccgacg agcttgtcgc cgaccagccg ggcgatgccg
     8221 ggccgggcga tgcggggccg ggcgatgcgg tgtcgggcga cgctgggccg ggcgggcggg
     8281 cccggcgcgt gctcgaggtc atcacccgcg acctcggttt cgcgccccgc accgacccgg
     8341 cggcaccaca cgtgatcctg ttgcggcact gtccctttgc cgccgccgcg tcgcaggccc
     8401 gagagatcat ctgcggcctg cacctcggcg tggccgaggg ggtatgccgc gccaccggcg
     8461 acgcgctcag cgtcgccgcc ctgcacgtcg ccgaccccca cgtcggcccc tgccgcctcg
     8521 aactcgcctg ac
//
LOCUS       DAOPJG010000148         8493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8843_length_8493_cov_8.061863, whole genome shotgun sequence.
ACCESSION   DAOPJG010000148 DAOPJG010000000
VERSION     DAOPJG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8843_length_8493_cov_8.061863"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKU75_12410"
     CDS             complement(<1..595)
                     /locus_tag="PKU75_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPF81727.1"
                     /translation="MKNRPVRVGLQLHPQGADYAHIRESVAAAEELGVDVLFNWDHFF
                     PLYGDADGKHFECWTMLAAWAEQTQRVQIGALVTCNSYRNPDLLADMARTVDHISAGR
                     LVLGIGSGWFERDYTEYGFDFGTAGSRLDDLAAALPRIEKRWAALNPPPTRQIPVLIG
                     GGGEKKTLRLVARHADIWHTFAQGEELAHKMEVLRGHC"
     gene            complement(623..1237)
                     /locus_tag="PKU75_12415"
     CDS             complement(623..1237)
                     /locus_tag="PKU75_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPF81728.1"
                     /translation="MSDQPSTPARRDLGLVFLGDSLVTGVGDPKGQGWVTRVVARSQT
                     PDVDLTAYNLGVRGATSSDVLERYRTEVPARFEGRGERRLVISAGTHDIYTGMSVARH
                     RLNLATILDEAASSGVAAFVVSPSPRADAQSNALLDVFVDAQADVCSRRGVPFVDAYR
                     PLLGHDQWESDLALATVEGHPGQAGYGLIAWLVLNGGWREWLDI"
     gene            complement(1248..2201)
                     /locus_tag="PKU75_12420"
     CDS             complement(1248..2201)
                     /locus_tag="PKU75_12420"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPF81729.1"
                     /translation="MESSRGRVVVIGSLNVDQVVRVADHPKPGETITGEFSGLFAGGK
                     GANQAVAAAEAGADVVMVGCVGADDQGRAYVARLAGLGLDVGQIEVSHRQPTGTAFIT
                     VDDAGENTVIVVAGANTDVGAGWPGSFPELTEADVVVLQLEIPLGTVAKAAELAREAG
                     ARVVLNIAPYAELPPDVLALADPIVANEHEAMALADSGAGVGSLLMTLGANGAVWDGV
                     GSPAEAVAPDLIVDTTGAGDAFVGALAAALADGASREEALQQAQIAGAEAVTHWGAQR
                     DGELVLAGRDAEPARGAERGAERAAYGPPGRADSADPPPDV"
     gene            complement(2230..3216)
                     /locus_tag="PKU75_12425"
     CDS             complement(2230..3216)
                     /locus_tag="PKU75_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPF81730.1"
                     /translation="MPALPVILDVDTGVDDACALLLAALHPDLDLRAVTCVGGNAPLA
                     DVVRNTLTVLTTAGRDDVPVAAGADRPLLEDPVDARHVHGQDGMGDLDWPVAARAADD
                     RHAVELLRDHLLAAAATGERVTLIPLAPLTNIALLLRTYPAVAQGIERILFMGGAAAV
                     GNATASAEFNIFHDPEAAAIVFDSCADLGITLGMYGLDVFYEPRITRAQAAELIAADP
                     QGPAGLAGRLVTFQCDRFGGESATIGDAGAVAAVIAPDSLTTSKLPARVELTGRYTRG
                     RTIVDHRDWSGDLTHDPHGQSDVIVDVALAVDSARLSTLWLETMRESAREKV"
     gene            3296..4450
                     /locus_tag="PKU75_12430"
     CDS             3296..4450
                     /locus_tag="PKU75_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPF81731.1"
                     /translation="MRQRLLATGGLIVATLAGGTQPGAGAATSEPGLTARQATCVGQL
                     NKDLSTKARVGQLIWVGMYASDPHGADRYVADYDVGGVVLLGGFHSGVSATRAATGHI
                     ASFADPRVKLVVAADQEGGYVQQLQGSGFSTIPTAYYQGRTWTPSTIQTRTAAWAGQL
                     KAAGVNVNLAPVADTVNKDFMPYNRPIGYYQRNYDYVADRVAIDVRAAVGGMHAAGVA
                     STVKHFPGLGRITNNTDTSSTGITDPSTWPGSAYLRPFAAGIDAGTEFVMVSNAYYAK
                     IDPARQAVFSPTVIEGLLRGQLGYQGVVITDDVGAAVAVSSTPVGSRATKFINAGGDI
                     VLTATPSSVPTMLEAIRAARATSPTFKAKLDAAVLRILRTKTAMGLTSCS"
     gene            complement(4457..5743)
                     /locus_tag="PKU75_12435"
     CDS             complement(4457..5743)
                     /locus_tag="PKU75_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPF81732.1"
                     /translation="MTTSSSGASGRRRRALLAGLITGTSVALGAGACTPSGTPSTGAS
                     STSASRPPATSASTTAGTATAGETTSNSSSSSSASTSADPIGACVDTAVAGLSEEEQI
                     GQLLMVGLDAGAGARGVDSAVTEHHVGGVILLGGWSGAATVTAVTEHLNSIATGPKLF
                     LAADQEGGQVQQLKGAGFSTIPSATQQATLGPAALREAATDWAQELVDVGINVNLAPV
                     ADTVPADIGTANGPIGRYHREYGSTPAAVIPPMTAFLEGMHDGEVMTAVKHFPGIGRI
                     RGNTDSSATGITDPTTTLDDPYLEPFRAGIDAGTDFVMVSSAAYPKIAAAQALFAPEI
                     VTDLLRTRLGYDGVVITDDVGAAAAVAATPIGERATKFIAAGGDIVLTAKASDIPVMA
                     RAVKAELADEEFAAKAGAALERVLTLKARRGLLDCS"
     gene            complement(5740..6783)
                     /gene="tsaD"
                     /locus_tag="PKU75_12440"
     CDS             complement(5740..6783)
                     /gene="tsaD"
                     /locus_tag="PKU75_12440"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPF81733.1"
                     /translation="MPDEPLVLGIETSCDETGIGIVRGETLLVDAIASSVEEHARFGG
                     VVPEVASRAHLEAMVPTIERACARSGVTLRDLDAIAVTSGPGLAGTLMVGVAAAKALA
                     LALGKPLYGVNHLAAHVAVDIVEHGALPEPVLAMLVSGGHSSLLLVPDVTDDVRPLGA
                     TIDDAAGEAFDKVARVLGLPFPGGPHIDRAARDGQIVIDFPRGLTSGRDMERHRFDFS
                     FSGLKTAVSRWVESQRAAGVEIPVADVAASFQEAVVDVLTRKAILACQEHGVGDLQIG
                     GGVAANSRLRAMAQSRADAAGIRLRVPRPALCTDNGAMVAALGAQLVAKDRPASGLDL
                     PADSSLPVTVVHP"
     gene            complement(6776..7267)
                     /gene="rimI"
                     /locus_tag="PKU75_12445"
     CDS             complement(6776..7267)
                     /gene="rimI"
                     /locus_tag="PKU75_12445"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPF81734.1"
                     /translation="MNGIRLRDLTWPDLPEIEAIERAVFPDDAWASASWWHELATRPR
                     RDYIVARDAERVLGYAGTDHGGEVSDVMTIAVSAAARGRGLGRILLQELENRAILRGA
                     TGMMLDVRADNAAALGLYTGAGYDLIQTRRRYYQPGDVDALVLRKFLTTSEPRPDTER
                     AHA"
     gene            complement(7264..7962)
                     /gene="tsaB"
                     /locus_tag="PKU75_12450"
     CDS             complement(7264..7962)
                     /gene="tsaB"
                     /locus_tag="PKU75_12450"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPF81735.1"
                     /translation="MTHGGPALRILAFDTATSAITAAVRDGDQVRAEEAVLDNRRHTE
                     ALAPVIERVLTASGTRMTEVTHIAVGIGPGPFTGLRVGVVTALVLGSALQVPVHGVCS
                     LDALAEALAGRHDGDLLVATDARRTEVYWARYAAAEGHATRVTDPAVTKPADLPPDAR
                     TLPCAGRGPLLYPDILVNAVAPQDVSAGVLAALAASEIHRGVRTEPVPMYLRRPDAQE
                     PAAPKPALPTSGRP"
     gene            complement(7959..8468)
                     /gene="tsaE"
                     /locus_tag="PKU75_12455"
     CDS             complement(7959..8468)
                     /gene="tsaE"
                     /locus_tag="PKU75_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPF81736.1"
                     /translation="MTRFRTGAVTLATPEDTQAFGRALATLLRAGDLVILTGDLGAGK
                     TTLTQGIGAGLNVRGPITSPTFVIARVHPTLAAGPALVHVDAYRLGSFAELDDMDLDA
                     DIADSVTLVEWGEGLAEDLTDSHLAIRMTADPATETRRVEITAVGDRWQTTGSRSALQ
                     ALTSEDDAS"
BASE COUNT         1246 a         2917 c         3100 g         1230 t
ORIGIN      
        1 cacaatgacc gcgcaggacc tccatcttgt gggcgagttc ctccccctgg gcgaaggtgt
       61 gccagatgtc ggcatgccgc gcgacgagcc ggagggtttt cttctcgccg ccgccgccga
      121 tgaggacagg gatctggcgc gtcggcggtg gattcagtgc agcccaacgc ttttcgatgc
      181 gcgggagcgc cgctgccaaa tcgtcgagcc ggctaccggc cgtgccgaaa tcgaagccgt
      241 attcggtgta gtcccgctcg aaccagccgg agccgatgcc caggacgagc cggccggcgc
      301 tgatgtgatc gacggtgcgg gccatgtcgg cgagcaggtc ggggttgcga tagctgttgc
      361 aggtgacgag cgcgccgatc tgcacgcgct gggtctgctc cgcccacgcg gccagcatcg
      421 tccagcactc gaaatgcttg ccgtcggcgt cgccatagag cgggaagaag tggtcccagt
      481 tgaacagcac gtcgacgccc agttcctcgg ccgccgcgac cgactcacgg atgtgggcgt
      541 agtcggcgcc ttgcggatgc agttgcaggc caacgcgaac ggggcggttc ttcacccctc
      601 gaccctcttg gcagggcaag actcagatgt cgagccactc gcgccagccg ccgttgagga
      661 cgagccaggc gatcaatccg tagccggcct ggcccgggtg cccctcgacg gtcgcgagcg
      721 cgaggtcgga ctcccactga tcgtgaccga gcagcgggcg gtaggcgtcg acgaagggca
      781 cgccccggcg ggaacagacg tcggcctgcg cgtccacgaa gacgtcgagc agggcgttcg
      841 actgggcgtc ggcgcgcggc gacgggctga cgacgaaggc cgccacgccg ctgctcgccg
      901 cctcgtcgag aatcgtggcg aggttgagac ggtgacgggc caccgacata ccggtgtaga
      961 tgtcgtgggt gccggccgag atgaccaggc gccgctcccc gcgaccctcg aaccgggccg
     1021 gcacctcggt gcgatagcgc tcgagcacat cactgctcgt cgccccgcgc acgcccaggt
     1081 tgtatgcggt cagatccaca tcgggggtct ggctgcgcgc gaccacccgg gtcacccaac
     1141 cctggccctt ggggtcgccg acgccggtca ccagcgagtc gccgaggaag acgagtccga
     1201 gatcgcggcg ggcgggggtc gagggttggt cactcacggc ggcaactcta aacgtcgggg
     1261 ggaggatccg ccgagtcggc tcggcccggc ggaccgtatg ccgcccgctc cgccccgcgc
     1321 tccgccccgc gcgccggctc ggcgtcgcgg ccggcgagca ccagttcgcc gtcgcgttgg
     1381 gcgccccagt gggtgaccgc ctcagcgccg gcgatctggg cctgctgcag ggcctcctcg
     1441 cgggaggccc cgtccgccag tgcggcggcg agggcgccga cgaaggcgtc gccggcgccg
     1501 gtggtgtcga cgatcaggtc cggggccacg gcctcggcgg gagagccgac cccgtcccac
     1561 acggcgccat tggccccgag ggtcatcaac agcgacccga cgccggcccc ggagtcggcg
     1621 agcgccatcg cctcgtgctc gttggcgacg atcggatcgg cgagggcgag cacatcgggc
     1681 ggcaactcgg catacggcgc gatgttcaac accacgcgcg cccccgcctc ccgggcgagt
     1741 tcggccgcct tcgcgaccgt gcctagcggg atttcgagtt gcaggacaac gacatccgcc
     1801 tcggtcagtt cgggaaatga cccgggccac ccggcgccca cgtcggtgtt ggcgcccgcg
     1861 acgacgatca cggtgttctc gcccgcgtcg tcgacggtga tgaacgcggt gccggtcggc
     1921 tgacggtggc tcacctcgat ctggccgacg tcgagaccga gaccggccag ccgcgcgaca
     1981 tacgcccggc cctggtcgtc ggcgcctacg cagccgacca tgaccacgtc ggcgcccgcc
     2041 tcggcggccg cgacggcctg gttggcgccc ttgccgccgg cgaagagccc cgagaactcg
     2101 ccggtgatcg tctctcccgg tttgggatgg tcggcgacgc gcaccacctg gtcgacattg
     2161 agcgacccga tgacgactac ccgaccccgt gacgactcca cggtgagagc gtaaccgccg
     2221 cggcccgggt cagaccttct cgcgcgcgct ctcgcgcatc gtctccagcc acagcgtgct
     2281 caagcgggcg ctgtcgacgg cgagggccac gtcgacgatg acgtcggact ggccgtgcgg
     2341 gtcgtgggtg aggtcgccgg accagtcccg gtggtcgacg atggtgcgtc cgcgcgtgta
     2401 gcggccggtg agttcgaccc gagccggcag tttggaggtg gtcagggagt cgggggcgat
     2461 gaccgcggcc acggcgccgg cgtcgccgat ggttgcactc tcgccgccga agcggtcgca
     2521 ctggaaggtc acgagccggc cggcgagacc cgccgggccc tgtgggtcgg cggcgatcag
     2581 ctcggccgcc tgagcccggg tgatgcgcgg ctcgtagaag acgtccagcc cgtacatccc
     2641 aagcgtgatc ccgaggtcgg cgcaggagtc gaagacgatg gccgccgcct cggggtcgtg
     2701 gaagatgttg aactcggccg aagcggtggc attgcccaca gcagcggcac cacccatgaa
     2761 gaggatgcgt tcgatcccct gtgccacagc gggatacgtg cgcagcagca gggcgatatt
     2821 ggtcaggggc gccagtggta tgagggtgac ccgctccccc gtcgcggcgg cggcgagcag
     2881 gtggtcacgc aggagttcga cggcgtggcg atcgtcggcc gcccgggccg ctaccggcca
     2941 gtcgaggtca cccatgccgt cctggccgtg cacgtggcgg gcgtcgacgg ggtcctccag
     3001 gagcggccgg tcggctccgg cggcaaccgg gacgtcgtcg cgaccggccg tcgtcaggac
     3061 ggtgagggtg ttgcggacga cgtccgcgag cggagcattg cccccgacac aggtgacggc
     3121 gcgcaggtcg aggtcagggt gcagcgcggc caggagcagg gcgcaggcgt cgtcgacacc
     3181 ggtgtcgacg tcgaggatca caggaagagc aggcacaacc gtcaatggta agtccgcgca
     3241 ggcgcggcac ccgtcgggtc cgagaccgtt cgtcatgact ggctatggtc gatgcatgcg
     3301 tcaacgcctg ctcgccaccg gtggcctcat cgtcgcgacc ctcgccggag ggacccagcc
     3361 cggggccggt gccgcgacct ccgagccggg tctgaccgcc cggcaggcga cgtgcgtcgg
     3421 gcagctcaac aaggacctgt ccaccaaagc ccgcgtggga caactgatct gggtcggcat
     3481 gtacgccagc gacccgcatg gcgccgatcg gtatgtcgcg gactacgacg tcggcggcgt
     3541 cgtcctgctg ggcggcttcc actccggcgt ctcggccacc cgcgccgcga ccgggcatat
     3601 cgcgagcttc gccgacccgc gggtgaaact ggtcgtcgcg gccgatcagg agggcggcta
     3661 tgtccagcag ctgcagggca gcggcttctc gacgatcccg accgcctact accagggacg
     3721 cacctggacc ccgtcgacca tccagacccg gacggccgcg tgggccggtc agctcaaggc
     3781 ggccggcgtc aacgtcaacc tcgccccggt cgccgacacg gtgaacaagg atttcatgcc
     3841 gtacaaccgg ccgatcggct actaccaacg caactatgac tacgtcgccg accgcgtcgc
     3901 gatcgacgtg cgcgcggcgg tcggcggtat gcacgcggcg ggagttgcct cgaccgtcaa
     3961 gcacttcccc ggcttgggcc ggatcaccaa caacaccgac acctcctcga ccggtatcac
     4021 cgatccctcc acctggcccg ggtcggcgta cctgcgcccg ttcgcggccg gcatcgacgc
     4081 cggcaccgag ttcgtgatgg tgagcaatgc ctactacgcc aagatcgacc cggcacgcca
     4141 agcggtgttc tctcccaccg tgatcgaggg tctgctgcgt gggcaactcg gctatcaggg
     4201 tgtcgtgatc accgacgacg tcggcgccgc ggtggccgtg tcctcgacac ccgttgggtc
     4261 gcgcgccacc aagttcatca acgcgggagg cgatatcgtc ctcaccgcga cgccctccag
     4321 tgtgccgacg atgctcgagg ccatccgcgc ggcccgggcg acgagcccga cgttcaaggc
     4381 caagctcgac gcggccgtgc tgcgcatcct gcgcaccaag acggccatgg gattgaccag
     4441 ctgttcgtga cccggttcag gagcagtcga ggagtccgcg gcgggccttg agcgtcagga
     4501 cgcgctcgag tgcggcgccg gccttcgcgg cgaattcttc gtcggccagc tcggccttga
     4561 cggcccgcgc catcaccggg atgtcactcg ccttcgcggt caggacgata tctccgccgg
     4621 cggcgatgaa ctttgtcgcg cgctccccga tgggggtggc ggcaaccgcc gcggccgcgc
     4681 cgacgtcgtc ggtgatgacc acgccgtcat acccaagccg ggtgcgcaac aggtcggtga
     4741 cgatctcggg agcgaaaagt gcctgcgccg cagcgatctt cgggtatgcc gcggaggaga
     4801 ccatgacgaa gtcggtgccc gcgtcgatgc ctgcccggaa gggttcgaga tagggatcgt
     4861 cgagcgtggt cgtcggatcg gtgatgccgg tggcgctgct gtcggtgttg ccccggatgc
     4921 ggccgatgcc ggggaagtgt ttgacagcgg tcatcacctc accgtcgtgc atgccctcga
     4981 ggaaggcggt catcgggggg atgacggcag cgggcgtcga gccatactcg cggtggtaac
     5041 gcccgatcgg gccgttggcg gtgccgatgt cggcgggcac ggtgtcggcg acgggcgcca
     5101 ggttgacgtt gatgccgacg tcgaccagtt cctgcgccca gtccgttgcg gcttcccgta
     5161 gcgccgccgg accgagcgtg gcctgttggg tcgccgacgg gatggtgctg aaacccgccc
     5221 ctttcagttg ctggacctgg ccgccctcct ggtccgcggc gaggaagagc ttcggacccg
     5281 tggcgatcga gttcaggtgc tcggtcacgg ccgtcaccgt cgccgcgccg ctccagccgc
     5341 cgaggaggat gacgccaccg acatggtgct cggtgaccgc actgtcgacg ccccgggcgc
     5401 cggcgccggc gtcgagtccg accatgagca gctgcccgat ctgctcttcc tcgctcaggc
     5461 cggcaacggc cgtgtcgacg caggcgccga tcgggtcggc ggacgtgctg gcagaggaac
     5521 tgctggacga gttcgatgtc gtctcccccg ccgtggcggt cccggctgtc gtgctcgctg
     5581 acgtggcggg cggccgcgac gcagaggtgc tcgatgcgcc ggtcgacggc gtgccgctgg
     5641 gggtgcacgc gccggcgccg agcgcgacgc tcgtgccggt tatcaggccg gcgagcagcg
     5701 cacgccggcg ccggccgctc gctccgctcg aggatgtcgt catgggtgca ccaccgtcac
     5761 aggcagggag gagtcggcgg gtaggtcgag accagacgcg gggcggtcct tggcgaccag
     5821 ctgggcgccc agggcggcga ccatggcgcc attgtcggtg cacagggccg gacggggcac
     5881 ccgcagccgg atgccggcgg cgtcggctcg ggactgcgcc atcgcccgca ggcgcgagtt
     5941 ggccgcaacc ccaccgccga tctgcaagtc gccgacgccg tgctcctggc aggccagaat
     6001 cgccttgcgg gtcaacacat ccacgacggc ttcctggaaa gacgccgcga catcggccac
     6061 ggggatctcc accccggcgg cgcgctggct ctccacccac cgggacacgg cggtcttcaa
     6121 accggagaag gagaagtcga agcgatgacg ctccatgtcg cggccgctcg tcagcccacg
     6181 cgggaagtcg atgacgatct ggccgtcccg ggcggcgcgg tcgatgtgcg gcccgccggg
     6241 gaagggcagc ccgagcactc gggcgacctt gtcgaaggcc tcgccggcgg cgtcgtcgat
     6301 cgtcgcgccg aggggccgca cgtcgtcggt gacatcgggc acgagcagca gcgaggagtg
     6361 cccgccggag acgagcatgg ccagcaccgg ttcgggcaag gcgccgtgct cgacgatgtc
     6421 gacggcgacg tgcgcggcaa gatgattgac cccgtacagc ggcttgccga gcgccaaggc
     6481 gagggctttc gcggccgcga ccccgaccat gagcgtgccg gcgagcccgg ggccgctcgt
     6541 cacggcgatc gcgtccagat cgcgcagcgt cacacccgat cgggcacagg cacgctcgat
     6601 ggtcggcacc atcgcctcga ggtgggcccg gctggccacc tcgggcacga ccccgccgaa
     6661 gcgggcgtgc tcctcgacgc tgctggcgat ggcatcgacc agcagcgtct cgccgcgcac
     6721 gatcccgatg ccggtctcgt cgcaggaggt ctcgatgccg aggaccaggg gttcatcagg
     6781 catgggcacg ctccgtgtcg gggcgaggtt cgctcgtcgt caggaacttg cgcagcacga
     6841 gcgcgtcgac gtcaccgggt tggtaataac ggcgccgggt ctggatcagg tcgtagccgg
     6901 cgccggtgta gaggccgagc gccgcggcgt tgtcggcccg cacgtcgagc atcatgcccg
     6961 tcgcacctct gaggatcgcc cggttttcca actcctgcag caagatccgg cccaacccgc
     7021 ggccgcgcgc ggcggccgac accgcgatcg tcatcacgtc cgagacctcg ccgccatgat
     7081 cggtgccggc gtagccgagc acccgctcgg cgtcgcgggc gacgatgtag tcgcgccgcg
     7141 gccgggtcgc gagttcgtgc caccaggagg cgctggccca ggcatcgtcg ggaaagaccg
     7201 cgcgctcgat cgcctcgatt tcgggcagat cgggccaggt caggtcgcgc agccgtatgc
     7261 cgttcatggc cgcccactcg tcggcagggc gggtttgggc gcggcgggtt cctgggcgtc
     7321 cgggcgccgc aggtacatcg gcaccggctc cgtgcggacc cctcggtgaa tctcgctggc
     7381 cgcgagcgcg gccagcacgc ctgccgaaac gtcttggggt gcaactgcgt tgaccaggat
     7441 gtcggggtag agcagcgggc cgcggccggc gcacggcagg gttcgcgcgt ccggcgggag
     7501 atcggccggc ttggtgacgg cggggtcggt gacgcgcgtg gcgtggccct cggccgcggc
     7561 ataccgtgcc cagtagacct ccgtgcgccg ggcgtcggtg gcgaccaaaa ggtcgccgtc
     7621 gtgccgcccc gcgagggcct cggcgagggc gtcgagggaa cacacgccgt gcaccgggac
     7681 ctgcagcgcg gagccgagca cgagcgcggt gacgacgccg acgcgcaatc cggtgaaggg
     7741 gccggggccg atgccgacgg cgatgtgcgt gacctcggtc atgcgggtgc cggaggccgt
     7801 cagcacccgc tcgatgacgg gcgcgagtgc ttcggtgtgc cgccggttgt cgagcacggc
     7861 ctcctcggcg cggacctggt cgccgtcgcg cacagcagcg gtgatggccg aggtcgcggt
     7921 gtcgaaggca aggatgcgca gggcggggcc accgtgggtc atgatgcgtc gtcctcggac
     7981 gtcaaggcct gcaacgctga ccgggaaccc gttgtctgcc aacgatctcc gaccgcggtg
     8041 atctccacgc ggcgggtctc ggtcgcggga tcggcggtca tccggatcgc gaggtggctg
     8101 tcggtcaggt cctcggccag gccctcgccc cattcgacga gcgtcaccga gtcggcgatg
     8161 tcggcgtcga ggtccatatc gtcgagttcg gcgaacgacc cgaggcgata ggcgtcgacg
     8221 tgcaccagtg cgggaccggc ggccagcgtc gggtgcacgc gagcgatgac gaacgtgggc
     8281 gacgtgatcg ggccacggac gttcaggccc gcgccgatcc cctgggtaag ggtggtcttc
     8341 ccggcaccca ggtctccggt gaggatcacg agatcaccgg cccgcagcag cgtggccagc
     8401 gcccgcccga aggcctgggt gtcctcggga gtggcgagcg tgaccgctcc cgtgcgaaaa
     8461 cgcgtcatac cggatccttg gtccgcgact gtt
//
LOCUS       DAOPJG010000149         8459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_8908_length_8459_cov_8.274750, whole genome shotgun sequence.
ACCESSION   DAOPJG010000149 DAOPJG010000000
VERSION     DAOPJG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8908_length_8459_cov_8.274750"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKU75_12460"
     CDS             complement(<1..190)
                     /locus_tag="PKU75_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPF81737.1"
                     /translation="MNPILIGADLIAITALVFALFLPRHGRRDLIVAYLGVNVGVLAV
                     ASALATSTVSAGLGLGLFG"
     gene            509..805
                     /gene="gatC"
                     /locus_tag="PKU75_12465"
     CDS             509..805
                     /gene="gatC"
                     /locus_tag="PKU75_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPF81738.1"
                     /translation="MSELTRADVAHLAGLARIALSDAELDKMVGELGIILESVATVQN
                     APIADVAPMSHPMPLMNVTRPDEVRPSLTPQAALAGAPEQDEQRFSVPRILDED"
     gene            851..2380
                     /gene="gatA"
                     /locus_tag="PKU75_12470"
     CDS             851..2380
                     /gene="gatA"
                     /locus_tag="PKU75_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPF81739.1"
                     /translation="MTNEIITSSAADLAARLAAGEISSVEVTQAHLDRTSAVDGAVHS
                     YLHVDFDGALATAREIDTRRAAGEQLHPLAGVPIAVKDVMTTKGLPTTCGSRILQGWI
                     PPYDSTVVAHLKAAGMPILGKTNMDEFAMGSSTEHSAYGPSHNPWDLERIPGGSGGGS
                     SSVLASFQAPLATGTDTGGSIRQPAAVTGTVGTKPTYGGVSRYGLVALASSLDQAGPC
                     ARTVLDTAMLHEVMAGYDPMDSTSIDEPVPAIVEAARRADVTGLRIGIVKELTGEGFQ
                     AGVQQRFDESVQHLVDAGAEIVEVSCPSFTYALATYYLILPSEASSNLAKFDAMRYGL
                     RVPPEDIDDPSAEQVMAATRDAGFGDEVKRRIILGTYALSSGYYDAYYGSAQKVRRLI
                     SDEFTAAFEKADVLVSPTAPTTAFKLGDKLDDPMAMYLNDIATIPANLAGIPGMSLPS
                     GLAPEDGLPVGFQILAPAMQDQRLYAVGAALEARLLHAWGAPLLASAPDLTAVAKGTS
                     R"
     gene            2377..3897
                     /gene="gatB"
                     /locus_tag="PKU75_12475"
     CDS             2377..3897
                     /gene="gatB"
                     /locus_tag="PKU75_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPF81740.1"
                     /translation="MTQATTDATLSYDEALAAYDPVMGLEVHVELGTKTKMFCGCATT
                     FGAEPNSQVCPVCLGLPGALPVVNEKGVEGAIRIGLALNCEIAQWCRFARKNYFYPDM
                     PKNFQTSQYDEPISYDGWLDVELEDGTVYRVEIERAHMEEDTGKSLHVGGSTGRIHGA
                     THSLVDYNRAGIPLIEIVTKPMVGAGERAPEIARAYVATLRDLIKALGVSDVKMEQGS
                     MRCDVNLSLMPKGSNTFGTRSETKNVNSLRSVERAVRYEIQRHAAVLQSGGAVTQETR
                     HWHEDTGITTAGREKSDAEDYRYFPEPDLVPIAPSRESVEALRATLPEPPAARRKRLQ
                     SAWGYSDLEMRDVVNAGLVELIDETVTAGASPAGARKWWSGEIARRANGEGTDVPSYA
                     AQLGVTPAHVVELESLVTAGRLNDSMARQVWEGVLGGEGAPTAVADARGLELVQDTGA
                     LDAAVEKVIAANADVAQKVRDGKVQAAGALIGQVMKEMKGQADAAAVRELILAKLT"
     gene            4052..4615
                     /gene="ahpC"
                     /locus_tag="PKU75_12480"
     CDS             4052..4615
                     /gene="ahpC"
                     /locus_tag="PKU75_12480"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HPF81741.1"
                     /translation="MALINTPIKPFTAQAFHNGEYVEVSEKDVLGKWAIFFFYPADFT
                     FVCPTELGDLADQYDELQQLGVEVYSVSTDTHHVHAAWHKESETVGKIKYFMLGDPSG
                     VITNNFENMREGQGLADRATFLVDPDGIIQVMEVTCEGVGRNAAELVRKVKAAQYVRN
                     HPGEVCPAKWEEGADTLKPSIDLVGKI"
     gene            4630..6228
                     /gene="ahpF"
                     /locus_tag="PKU75_12485"
     CDS             4630..6228
                     /gene="ahpF"
                     /locus_tag="PKU75_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HPF81742.1"
                     /translation="MLDPALKDQLRTYLTNVRQPIELVSSLDDSAKSMEMAELLDEIA
                     GLSEQVTHVRNGADARRPSFRIVRAGTDVHVTFAGIPLGHEFTSLVLALLHVGGHPPK
                     VDEDTAEQVKALAKPLHFETYMSLSCQNCPDVVQALNIMSVLNPEHISHTAIEGGLFQ
                     DEVADKGIQAVPTVYLNGELFDSGRMSLEQILGKVDEHADTKAAAKIDAMAPFEVLII
                     GGGPAGASAAIYTARKGIRTGIVAERFGGQLNDTMAIENYPSVSHTEGPRMAADLEAH
                     VRSYGVEVVLGQHAAKLVPAEQPGGYHRVELASGATLQGRTVIIATGARWRTMGCTGE
                     EDYRNKGVTFCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLAGIVGHVTVVEFLDQLRA
                     DDVLVDKLASLPNTEVRLGRRTTAVVGDGAKVTGLTTEPREGGDAESIDLDGVFVQIG
                     LLPNAEWVKGTLEVSDRGEIVVDERCVTSVPGVFAAGDVTVIPFKQITIASGTGATAA
                     LSAFDYLIRTPAPEPTAKEPALAG"
     gene            complement(6299..7078)
                     /locus_tag="PKU75_12490"
     CDS             complement(6299..7078)
                     /locus_tag="PKU75_12490"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HPF81743.1"
                     /translation="MSNAVDDILKSLDMNQLADMVGADPNTVEQAAATALPALFGGLD
                     ANAQDPMGANSILQALGQHSGSLLDGGIDLGQIDPQDGAAITSHIFGNNEEAVYNQLG
                     GYGAGGGLGGSLFKRLLPILAPIVMSYIMKHMTQRTGGAAGGSGGGLGDILGQVLGGG
                     SAGQTQQSSGGGGLGDILGQVLGGSQQSQPQDTNVFPDEVQQPTQQNTQAGSGQQPNL
                     DAILKDVLGGAASSTSNKTQSAGGGSIIGDILGGLLGGGRR"
     gene            7232..7759
                     /locus_tag="PKU75_12495"
     CDS             7232..7759
                     /locus_tag="PKU75_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF81744.1"
                     /translation="MTTAAMDRTLILVRHAATAHQGPGEDDHDRELTPQGHLDARAAG
                     AWLRTHDFGVDQVLCSSAERTRQSTEGMWAGGCCEADVHYDGRIYNASPETLLDVLRE
                     ADDAANVVMLVGHAPGVPALASLLADGEGNDSAHECLAAGFPTTGIAVLRFAGPWRDL
                     AFGGARLERFHLARA"
     gene            7820..>8459
                     /locus_tag="PKU75_12500"
     CDS             7820..>8459
                     /locus_tag="PKU75_12500"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPF81745.1"
                     /translation="MPELRSRTSTHGRTMAGARALWRATGMTDDDFGKPIVAIANSFT
                     QFVPGHVHLKDLGQLVAGAVAAAGGVAKEFNTIAVDDGIAMGHSGMLYSLPSRELIAD
                     AVEYMVNAHCADALVCISNCDKITPGMLLAALRLNIPVVFVSGGPMEAGKTVAVEGIV
                     HEKIDLIDAMIASSNDAVSDEQLDTIERSACPTCGSCSGMFTANSMNCLTEAI"
BASE COUNT         1415 a         2991 c         2786 g         1267 t
ORIGIN      
        1 ccccgaagag ccccagaccc agacctgcgc tgaccgtgga ggtggccagg gccgacgcga
       61 cggcgaggac accgacgttg acgccgaggt aggcgacgat caggtcgcgg cgtccgtggc
      121 ggggcaggaa gagggcgaag acgagggccg tgatggcgat caggtcggcg ccgatgagga
      181 tcgggttcat ggtcgtggct cctggtgtgt ctgttggcgt ttcgtgccgt ggtgacacct
      241 atttcgcccg gccaacccat cgagaccccc agcaacacct gtgccccacc tgtgaactgc
      301 accccacgaa gttctgcgct cctccgtcgc tccgatactt cgcggggaca ccgaaacgtc
      361 accccacgga gcgcaccccc gtcgagtgag cgcgacacgc ccatggtccg gcccggaact
      421 gggcgtgtcg cgctcactcg acagaggtgg tctcgggtgt cgcgcggggg agccggacca
      481 tggcgcggag gcaaatagac tgaccggcat gtccgaactg acccgcgccg acgtggcgca
      541 cctggccggg ctggcccgca tcgcgctgtc cgacgccgaa ctggacaaga tggtgggcga
      601 gctggggatc atcctcgagt ccgtcgcgac ggtgcagaac gccccgatcg ccgacgtggc
      661 gccgatgtcg cacccgatgc cgctgatgaa cgtcacccgc cccgacgagg tccgcccgtc
      721 gctgaccccg caggccgccc tggccggagc gcccgagcag gacgagcaac gcttctccgt
      781 gccccgcatc ctggacgaag actgatctct ggaccaagca gactgagcgg acgaggacga
      841 aaacctaact gtgacaaacg aaatcatcac ttccagcgct gccgacctgg ctgcccgcct
      901 ggccgccggc gagatctcct ccgtcgaggt cacccaggcg cacctggacc gcacctccgc
      961 cgtcgacggc gccgtgcact cctatctgca cgtggacttc gacggcgccc tggcaaccgc
     1021 ccgcgagatc gacacccgcc gcgctgcggg cgagcaattg caccccctgg ccggcgtgcc
     1081 gatcgcggtc aaggacgtca tgaccaccaa gggcctgccg acgacctgcg gctcgcgcat
     1141 cctccagggc tggatcccgc cctacgacag cacggtcgtc gcgcacctca aggccgccgg
     1201 tatgccgatc ctcggcaaga ccaatatgga cgagttcgcg atgggctcct ccaccgagca
     1261 ctcggcatac ggtccctcgc acaacccgtg ggacctcgag cgcatccccg gcggctccgg
     1321 cggcggctcg tcctcggtgc tcgcgagctt ccaggcgccg ctggccaccg gcaccgacac
     1381 gggcggctcg atccgccagc ccgcggccgt caccggcacg gtcggcacca agccgaccta
     1441 cggcggcgtc tcccgctatg gccttgtcgc acttgccagt tcgctcgacc aggccggccc
     1501 atgcgcccgc accgtcctcg acaccgccat gctgcacgag gtcatggccg gctacgaccc
     1561 gatggactcc acctccatcg acgagcccgt cccggccatc gtcgaggcgg cccgccgcgc
     1621 cgacgtcacg gggctgcgca tcggcatcgt caaagaactg accggcgagg gtttccaggc
     1681 cggggtgcag cagcgcttcg acgagtccgt ccagcatctc gtcgacgccg gcgccgagat
     1741 cgtcgaggtc tcctgcccca gcttcaccta cgcgctcgcg acctactacc tcatcctgcc
     1801 ctccgaggcg agcagcaatc tcgcgaagtt cgacgccatg cgctacgggc tgcgcgtgcc
     1861 gccggaggac atcgacgacc cgagcgccga gcaggtcatg gccgccaccc gtgatgccgg
     1921 cttcggcgac gaggtcaagc gccggatcat tctcggcacc tatgcgctct ccagcggcta
     1981 ctacgacgcc tactacggct ccgctcagaa ggtgcgccgc ctgatctccg acgagttcac
     2041 ggccgcgttc gagaaggccg acgtcctcgt cagcccgacg gcgccgacga cggcgttcaa
     2101 gctcggcgac aagctcgatg acccgatggc gatgtacctc aacgacattg ccaccatccc
     2161 ggccaacctg gccggcatcc ccggcatgtc gctgccgagc gggctggcac ccgaggacgg
     2221 cctgccggtc ggcttccaga tcctcgcgcc cgccatgcag gaccagcggt tgtatgccgt
     2281 cggcgccgcc ctcgaggccc gcctcctgca cgcctggggt gccccgctcc ttgcgtccgc
     2341 acccgatctc accgcagtag cgaaggggac ctcccgatga cccaggcgac gacggacgcg
     2401 acgctctcct atgacgaggc gctggcggca tacgacccgg tgatgggtct ggaggtgcac
     2461 gtcgaactcg gcaccaagac caagatgttc tgcgggtgcg cgacaacctt tggcgcagaa
     2521 cccaattcgc aggtctgccc ggtctgtctc ggcctgcccg gcgcgctgcc ggtcgtcaac
     2581 gagaagggcg tcgaaggcgc gatccgcatc ggtctcgccc tcaactgcga gatcgcgcag
     2641 tggtgccggt tcgcccggaa gaactacttc tatccggaca tgcccaagaa cttccagacc
     2701 tcgcaatacg acgagcccat ctcctatgac ggctggctcg acgtcgaact cgaggacggg
     2761 acggtctatc gcgtcgagat cgagcgcgcg cacatggagg aggacaccgg caagtcgttg
     2821 cacgtcggcg gatccaccgg ccgcatccac ggcgccaccc attcgttggt cgactacaac
     2881 cgcgccggca taccgctgat cgagatcgtc accaagccca tggtcggggc gggggaacgg
     2941 gcaccagaga tcgcccgcgc ctatgtcgcg acgctgcgtg acctgatcaa ggcgctcggc
     3001 gtctcggacg tcaagatgga gcagggctcg atgcgctgcg acgtcaatct ctcgctgatg
     3061 cccaagggca gcaacacgtt cggcacgcgc agcgagacca agaatgtcaa ctcgctgcgg
     3121 tcggtcgagc gtgcggtgcg ctacgagatc cagcgtcatg cggccgtcct gcagtccggc
     3181 ggtgcggtca cccaggagac gcgccactgg cacgaggaca ccggcatcac cactgccggg
     3241 cgggagaagt cggacgcgga ggactaccgc tatttccccg agccggacct ggtgccgatc
     3301 gccccgtccc gcgagagcgt cgaggcgttg cgagcgaccc tgcccgagcc gcccgcggcg
     3361 cggcgcaagc ggctccaatc cgcctggggc tacagcgatc tcgagatgcg cgatgtcgtc
     3421 aacgccggtc tggtcgagct catcgacgag acggtgacgg ccggcgcgtc accggccggc
     3481 gcccgcaagt ggtggtcggg tgagatcgcc cggcgcgcca acggcgaggg caccgatgtc
     3541 ccgtcgtatg ccgcgcagct gggcgtcact ccggctcacg tcgtcgagtt ggagtcgctc
     3601 gtcacggccg gccggctcaa cgactcgatg gcccgccagg tctgggaagg cgtgctcggc
     3661 ggcgagggcg ccccgaccgc cgttgccgat gcccgcggac tggagctggt gcaggacacc
     3721 ggcgccctcg acgcggcggt ggagaaggtc atcgccgcga atgccgatgt ggcgcaaaag
     3781 gttcgcgacg gcaaggtgca ggccgcgggc gccctcatcg gccaggtgat gaaggagatg
     3841 aagggccagg ccgacgcggc ggcggtccgc gaactcatcc tcgccaagct cacgtaggca
     3901 tcccacgaag ttctccgctc ctgcgtcgct cccgggcttc gcggaactcc ggagagcgga
     3961 ccccgcgccc ggcgggaagg cttgcatttg gcgcctcctt cggtggtaat ctggaatcac
     4021 tccagaaaaa ccaccgaagg agcttcatcg catggccctc atcaacaccc cgatcaagcc
     4081 gttcacggcg caggcgttcc acaacggcga atacgtcgag gtgagcgaga aggacgtcct
     4141 cggcaagtgg gcgatcttct tcttctaccc ggccgacttc accttcgtct gcccgaccga
     4201 actcggcgac ctcgccgacc agtacgacga gctgcagcag ctcggcgtcg aggtctactc
     4261 cgtctccacc gacacccacc acgtgcacgc cgcgtggcac aaggagtccg agaccgtcgg
     4321 caagatcaag tacttcatgc tcggtgaccc ctccggcgtc atcaccaaca acttcgagaa
     4381 catgcgcgag ggtcagggcc tggccgaccg cgccaccttc ctcgtcgacc ccgacggcat
     4441 catccaggtc atggaggtca cctgcgaggg cgtcggccgc aacgccgccg aactcgtccg
     4501 caaggtcaag gccgcccagt atgtccgcaa ccacccgggc gaggtctgcc ccgccaagtg
     4561 ggaagagggc gcggacaccc tcaagccgtc catcgacctc gtcggcaaga tctgaggctg
     4621 agtagaccca tgctcgaccc ggcactgaaa gaccagctgc ggacctatct gaccaacgtc
     4681 cggcagccga tcgagctcgt cagctcgctc gacgactcgg cgaaatccat ggagatggcc
     4741 gagttgctcg acgagatcgc cgggctgagc gagcaggtca cgcacgtccg gaacggggcc
     4801 gacgcccgtc gtccctcgtt ccggatcgtg cgggccggca ccgacgtgca cgtgaccttc
     4861 gccggcatac cgctcggcca cgagttcacg tcgctcgtcc tggccctgct gcacgtcggc
     4921 ggccacccgc cgaaggtgga cgaggacacg gcagagcagg tcaaggcgct ggccaaacca
     4981 ctgcacttcg agacctatat gtcgttgtcg tgccagaact gcccggacgt ggtgcaggcg
     5041 ctcaacatca tgagcgtcct gaacccggag cacatctcgc acactgcgat cgagggcggc
     5101 ctcttccagg acgaggtcgc cgacaagggc atccaggccg tccccaccgt ctacctcaac
     5161 ggcgaactct tcgactccgg ccggatgtcg ctggagcaga tcctcggcaa ggtcgacgag
     5221 cacgccgaca ccaaggctgc cgccaagatc gacgcgatgg cgcccttcga ggtcctgatc
     5281 atcggcggcg gtcccgccgg cgccagcgcg gcgatctata cggcgcgcaa gggaattcgg
     5341 accggcatcg ttgccgaacg cttcggcggg cagctcaacg acacgatggc gatcgagaac
     5401 tacccgagcg tctcgcatac cgagggtccg cggatggcgg ccgatctcga ggcccacgtg
     5461 cggtcgtatg gcgtggaggt cgtcctcggc cagcacgccg cgaaactcgt cccggccgag
     5521 cagcccgggg gctatcaccg cgtcgaactc gcctctggcg caacgctgca agggcgcacg
     5581 gtcatcatcg cgaccggtgc ccggtggcgg acgatgggct gcaccggcga ggaggactac
     5641 cgcaacaagg gcgtcacctt ctgcccccac tgcgacgggc cgctcttcaa gggcaagcgc
     5701 gtcgccgtca tcggcggcgg caactcgggc atcgaggccg cgatcgacct ggccggcatc
     5761 gtcggccatg tgacggtcgt ggagttcctc gaccagttgc gcgctgacga cgtcctcgtc
     5821 gacaaactcg cgagcctgcc caacaccgag gtccggctgg gccggcgcac cacggccgtc
     5881 gtcggtgacg gcgccaaggt taccggtctg accaccgaac cacgcgaggg cggcgacgcc
     5941 gagagcatcg acctcgacgg cgtcttcgtc cagatcggcc ttctgcccaa cgccgaatgg
     6001 gtcaagggca cgctcgaggt cagcgaccgc ggcgagatcg tcgtcgacga gcgctgcgtc
     6061 acctccgtcc ccggcgtctt cgccgccgga gacgtcacgg tcattccgtt caagcagatc
     6121 acgatcgcca gcggcacggg agcaacggca gccctctcgg ccttcgacta cctcatccgc
     6181 accccggccc cggagccgac ggcgaaggag cccgcactcg ccggctgagt cgagtcacgg
     6241 catacgacga ggggcccgga ccgatcggtc cgggcccctc gcgcgatctt cccggggttc
     6301 agcggcggcc gccgccgagg agaccaccga ggatgtcgcc gatgatggag ccgccaccgg
     6361 cggactgggt cttgttgctt gtgctcgacg cagcgccgcc gaggacgtcc ttgaggatcg
     6421 cgtcgaggtt gggctgctga ccgctgccgg cctgggtgtt ctgctgggtc ggctgctgga
     6481 cctcgtcggg gaagacgttg gtgtcctgcg gctggctctg ctgcgaaccg ccgaggacct
     6541 ggccgaggat gtcaccgagg ccaccgccgc cggagctctg ctgggtctgg ccggccgagc
     6601 cgccgccgag gacctggccg aggatgtcgc cgaggccgcc gccggagcca cccgccgcac
     6661 cgccggtgcg ctgggtcatg tgcttcatga tgtaggacat cacgatcggg gcgaggatcg
     6721 gcaggaggcg cttgaacagg ctgccaccga gaccgccgcc ggcaccgtag ccaccgagct
     6781 ggttgtagac cgcctcttcg ttgttgccga agatgtggct ggtgatggca gcgccgtcct
     6841 gggggtcgat ctgcccgagg tcgatgccgc cgtcaagcag gcttccgctg tgctggccga
     6901 gggcctggag gatggagttg gcgcccatag ggtcctgggc gttggcatcc agtccgccga
     6961 agagcgcggg gagcgcggtt gcggcggcct gttcgacggt gttggggtcg gcccccacca
     7021 tgtcggccag ctggttcatg tccagacttt tcaggatgtc gtcaacggca ttggacacag
     7081 gacgcacctc tcgttcgtgc gtcgttgctg acgccgggcc tcccccgcat cggtgcggtg
     7141 gcgatctccg ttcggatcaa cgtagcaact ggggggcgtc tattcccgct cagagcccgc
     7201 ggaagtgaac ggaaagaggc atgctagagg tatgacgacg gcagccatgg accggacctt
     7261 gatcctggtt cggcacgccg ctacggccca ccagggaccc ggcgaggacg accacgaccg
     7321 tgaactgact ccgcaagggc acctcgacgc ccgcgccgcc ggcgcgtggt tgcgcacgca
     7381 tgacttcggg gtcgatcagg ttttgtgctc cagcgccgag cgcacgcgcc agagcaccga
     7441 gggcatgtgg gccggcgggt gctgcgaggc cgatgtgcat tatgacgggc gcatctacaa
     7501 cgcctccccc gaaaccctgc tcgacgtcct gcgggaggcg gacgacgccg ccaatgtcgt
     7561 gatgctcgtc gggcatgcgc ccggggtgcc ggccctggcg agcctgctcg cggacggcga
     7621 aggcaacgac tcggcgcacg agtgcctcgc cgccggcttc cccacgacgg ggatcgccgt
     7681 attgcgcttc gccgggccgt ggcgcgacct cgccttcggt ggcgcgcgcc tcgagcgttt
     7741 ccacctcgcc cgggcctgac aacaaccagg tcgcggacgc gatctgggcc gtatgccgcg
     7801 cgggtgccag aatcgccgta tgccggaact gcgttcgcga acctccactc acggccggac
     7861 catggcggga gcccgcgccc tgtggcgtgc cacgggcatg accgacgacg acttcggcaa
     7921 gccgatcgtc gccatcgcca acagcttcac gcagttcgtg ccggggcacg tccacctcaa
     7981 ggatctcggg cagcttgtcg ccggcgcggt cgccgcagcc gggggagtgg ccaaggagtt
     8041 caacacgatc gccgtcgacg acggaatcgc catggggcac agcgggatgc tctactcgct
     8101 gccctcgcgc gaattgatcg ccgacgccgt cgagtacatg gtcaacgccc actgcgccga
     8161 cgccctcgtg tgcatctcca attgcgacaa gatcaccccg ggtatgctgc tcgccgccct
     8221 ccgcctgaac atcccggtgg tcttcgtctc cggtgggccg atggaggcgg gcaagacggt
     8281 cgccgtcgag ggcatcgtgc acgagaagat cgacctgatc gatgcgatga tcgcctcgtc
     8341 caatgacgcg gtcagcgacg agcagctcga caccatcgag cgctcggcgt gccccacgtg
     8401 cggatcgtgc tcgggcatgt tcaccgccaa ctcgatgaac tgcctcaccg aggcgatcg
//
LOCUS       DAOPJG010000150         8395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9000_length_8395_cov_7.527458, whole genome shotgun sequence.
ACCESSION   DAOPJG010000150 DAOPJG010000000
VERSION     DAOPJG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9000_length_8395_cov_7.527458"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="PKU75_12505"
     CDS             complement(<1..1469)
                     /locus_tag="PKU75_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF81746.1"
                     /translation="MPSLTSPRNWTLRSKLVASMVALFLTVSIGTGSLIVLATRDYLA
                     RQIDQDLKQTVARSTFGDHDDSHPRGGGPGGPLPGGGAGVLSLVLDADGQVRTDPRSG
                     SPINTAINVDGDQITLDAEQVERLSDVKAGGGPETVDLGSSAGTYRVMAVATSSGTAI
                     TGIPTLPITKTVKQLLGLVAAATLMGLAIVALGGAYLVRRNLEPLSRVAGTARAVSQR
                     SLESGEVALAERVPASDTDPNTEVGQVGLALNSMLDNVDGALRARQESETRVRQFVAD
                     ASHELRTPLASIRGYAELSRKEREPVPPNVTHALGRIESESLRMQGLVEDLLLLARLD
                     AGRPLDHDPVDLTLLAIDAVSDARAAAPDQVWGLDLPEEPIEVPGDRARLHQVLVNLL
                     GNARAHTPPGTRVTTALHRGSTTDPTRTDVVTLSVIDDGPGIPAALQPNVFERFTRGD
                     EARNRAAGSTGLGLSIVAAVAAAHGGRVGLRSRAGETAFV"
     gene            complement(1477..2205)
                     /locus_tag="PKU75_12510"
     CDS             complement(1477..2205)
                     /locus_tag="PKU75_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF81747.1"
                     /translation="MTQPATQKLQRLDGTPVRVLVVDDEANLTELLSMALRYEGWEVR
                     AAGTGTAAVKAARDFQPDAVVLDMMLPDFDGMEVLRRMRADDANVPVLFLTAKDAVED
                     RVAGLTAGGDDYVTKPFSLEEVVARLRALMRRTAVVADESSSVLVVGDLTMDEDSHEV
                     TRAGTEINLTATEFELLRYLMRNPKRVLSKAQILDRVWNYDFGGQANVVELYISYLRK
                     KIDAGRAPMIHTMRGVGYVLKPAE"
     gene            complement(2388..4193)
                     /locus_tag="PKU75_12515"
     CDS             complement(2388..4193)
                     /locus_tag="PKU75_12515"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPF81748.1"
                     /translation="MTDSTGTTNTALQAWVDEVAALTTPDKVVWITGTDEEIEGLNQE
                     LVASGTFVKLNDEKKPNSYWCASDPSDVARVEDRTYIASEKEEDAGPTNNWIDPAELK
                     ATMTELYKGCMKGRTMYVIPFVMGHLDATVPMFGVEITDSAYVVTNMKIMARIGSEVL
                     AKMEELDADFVKGLHSVGAPLADGEKDVPWPCNDTKYIAHFPETREIWSYGSGYGGNA
                     LLGKKCYALRIASAMARDDGWMAEHMLILKLTSPKGSVHYVAGAFPSQCGKTNLAMLE
                     PTIEGWKAEMIGDDIAWMRFGPDGRLYAVNPEFGLFGVAPGTGMNTNPMAMRTIEKGN
                     SIFTNVARTDDGDVWWEGMTPDKPEHLIDWKGNDWTPESDVVSSHPNSRFTTPAKQCP
                     MIAPEYDNPDGVPIDAILFGGRRATTVPLVYESRDWTHGTFVGATLSSETTAAATGAV
                     GVVRRDPMAMLPFIGYHAGDYMNHWLEIGKQADASKLPRIFQVNWFRKDEDGTWLWPG
                     FGDNSRVLKWVVERLDGDADAVETPVGLVPAPGAIDTTGLNMTDEQVAKAIAVNTEDW
                     LKELPLIEEWFAKFGDKLPAPLQAELDGLKERLNA"
     gene            4463..5962
                     /locus_tag="PKU75_12520"
     CDS             4463..5962
                     /locus_tag="PKU75_12520"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPF81749.1"
                     /translation="MTSRPGDGPAPARPVRPRWLQPPTWRGEAPTEPLPVRASLRRTP
                     YRNPRVRRTQEERVAMDLALNVGEVLLRCGASAAQTHTSVVAVSLAAGLERIDVDITL
                     QSLLMQVQTADGEVATRLRVVGPPRWDFGRLAAVHALVDDLVSGEVTVAQAVERMQEI
                     HRAPRTWSSWVVALATGLLAAGVAVVLGAGVLAAVLCIGSAILVDRLVGTLSGLGIPD
                     FYASAAGGFVATAIAYGAYALGYVAFWSITPADFAAMVAGGIIALLPARSIASAMEDV
                     VTGYPVTGTSRIFSVLFHTLGLIIGVGSALPFSVWVATRAGQSVAPPAVERLASTTVG
                     LPVALAGAAAIGIFGAITMQSRRRYVLPAGGLTVVGVLVTRLLTSDVGVGRVTAVGLT
                     AIIIGFLARVVALRMGAPALVLAVPGSFGLLPGLAIFVGLYRLLVTAGGDLSGSLTIQ
                     VGAQELISAVGVLIAIATGNTLGDLLASPLDHRASMRRTEAPDGAGERG"
     gene            6118..7047
                     /locus_tag="PKU75_12525"
     CDS             6118..7047
                     /locus_tag="PKU75_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006213755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81750.1"
                     /translation="MRFEQPTVELLLEAAHMSTVAGVGMRPAEIEREIASGRLQPKWS
                     QVAIDEDESLVGRALWWGRDAQVPLALDVWDARRDHPDTERILIELLEHGHAALGSDG
                     VDIPLPHTMRIPTTWRDIEAVHCDVAVKTNAAAAAGLVRVNERRQFQWDQGARATRRA
                     HRLRFETADDDAFLHLFARATRGSLDVMTRRELATAAAETVAVDEVAYYRSCPGDRGW
                     WRVAYDSRGNVVGMAIPSATPTSRNIGYLAVLPEHRGHGYVDDLLAYITDFHMGDGAS
                     RITATTDAVNTPMIEAFKRAGYQCVETRIDLER"
     gene            complement(7209..7694)
                     /locus_tag="PKU75_12530"
     CDS             complement(7209..7694)
                     /locus_tag="PKU75_12530"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPF81751.1"
                     /translation="MQLILRTVVIFLALWLVLRVAGKRQVAQMDAFDLVTIIVLGGVV
                     TQGILQEDYSLVSALVAIGLVTLLAVGLSVLTWRFPGADKIIEGEPTILLQDGRPDET
                     AMRAERVSMANLLAAAREHGIRDLEEIDLAVLEADGAVSFFTRRTGEDDQPPEHRAKA
                     L"
     gene            complement(7707..8225)
                     /locus_tag="PKU75_12535"
     CDS             complement(7707..8225)
                     /locus_tag="PKU75_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81752.1"
                     /translation="MAIQTLPEAMTGSEATRARNMRRIGLALFAIPVVAAAFGVFGPR
                     ETTGTASGSGYELTVHYPQATRSSIVAPFSFTVTKAGGFGDDPVVVRLDQAFVDRLDF
                     QNWYPVPSAEANDGRQVVYEFDPPVGDSLEVSLDARTGPNQGYSSDDYHLTVLDGDRD
                     AATVTFHTVFWP"
BASE COUNT         1206 a         2775 c         2970 g         1444 t
ORIGIN      
        1 acgaaggcgg tctcgccggc gcgggagcgc aggccgacgc ggccaccatg ggcggcggcg
       61 acggcggcca cgatcgacag gccgaggccc gtgctgccgg cggcccggtt gcgggcctcg
      121 tcgccccggg tgaagcgttc gaagacgttg ggctgcaagg cggccgggat gccggggccg
      181 tcgtcgatga cggacagggt gacgacatcg gtgcgcgttg gatcggtggt gcttccccga
      241 tgcagggcgg tagtcacccg ggtgccgggc ggagtgtggg cgcgggcatt gccgagcagg
      301 ttgaccagca cctggtgcag ccgcgcccga tcgccgggca cttcgatcgg ttcctcgggc
      361 aggtccaggc cccagacctg atcgggggcg gccgcccgcg cgtcgctgac ggcgtcgatg
      421 gccaggaggg tcaggtcgac cggatcgtga tcgagggggc gtccggcgtc gaggcgggcc
      481 agcagcaaca agtcttcgac gaggccctgc atccgcagcg actccgactc gatccggccc
      541 aacgcgtggg tgacattcgg cgggaccggc tcgcgctcct tgcgggacag ctcggcatac
      601 cctcgaatac tcgccagcgg ggtccgcagt tcgtgcgagg catcggcgac gaattgccgc
      661 acccgcgtct ccgactcctg ccgggcccgc agcgcaccgt cgacattgtc cagcatgctg
      721 ttgagggcca acccgacctg cccgacctcg gtgttgggat cggtgtcgct cgccggcacc
      781 cgctccgcca aggcgacctc gcccgattcg agcgagcgct gcgacacggc ccgggccgtg
      841 cccgccaccc ggctgagggg ctcgaggttg cggcgcacga gataggcgcc gccgagggcg
      901 acgatggcca ggcccatcag ggttgcggcg gcgacgagcc cgagcaactg cttgacggtc
      961 ttggtgatcg gcagggtcgg gatgcccgtg atggcggtgc cggaggacgt ggcgacggcc
     1021 atgacgcggt aggtgccggc gctcgagccg aggtcgaccg tctcgggtcc gccgccggcc
     1081 ttgacatcgg agagccgctc gacctgttcg gcgtccagag tgatctgatc cccgtcgaca
     1141 ttgatcgcgg tattgatcgg cgagccgctg cgggggtccg tgcgcacctg gccgtcggcg
     1201 tccaggacga ggctgagcac gcccgcaccg ccgccgggca ggggaccgcc gggcccgccg
     1261 ccacgcggat gactgtcgtc gtgatcgcca aaggtggacc gggcgacggt ctgtttgagg
     1321 tcctggtcga tctggcgcgc caggtagtcc ctcgtggcca gcacgatcaa ggatccggtg
     1381 ccgatgctga cggtcaagaa gagcgccacc atcgacgcga cgagtttgga gcgcaacgtc
     1441 cagttgcggg gggaggtgag cgacggcata cacgactcac tctgcgggct tgaggacata
     1501 gcccacgcca cgcatggtgt ggatcatcgg tgcccggccg gcgtcgatct tcttgcgcaa
     1561 gtaggagata tagagctcga cgacattggc ctgcccgccg aagtcgtagt tccagacgcg
     1621 gtcgaggatc tgtgccttgg acagcacccg cttggggttg cgcatcagat agcgcagcag
     1681 ctcgaactcg gtggcggtta ggttgatctc cgtgcccgcc cgggtgacct catggctgtc
     1741 ctcgtccatc gtcaggtcgc cgacgacgag caccgaggag gattcgtcgg cgacgaccgc
     1801 ggtgcggcgc atcagggccc gcagccgcgc gacgacctct tcgagggaga agggcttggt
     1861 gacatagtcg tcgccgcccg cggtcaggcc ggcgacccgg tcctcgacgg cgtccttggc
     1921 agttagaaag agcaccggga cgttcgcgtc gtcggcgcgc atccggcgca gcacctccat
     1981 gccgtcgaag tcgggcaaca tcatgtcgag cacgacggcg tcgggctgga agtcgcgagc
     2041 cgccttgacc gccgcggtgc cggtgccggc cgcgcggacc tcccagccct catagcgcag
     2101 cgccatcgag agcagttcgg tgagattggc ctcgtcgtcg acgacgagca cccgcaccgg
     2161 ggtgccgtcg aggcgctgca gcttctgggt ggccggctgt gtcatgcctc cagtgtctcc
     2221 cgccaaccct gggtatgtcg tggctcaccc ctgtgggaac actatgggtt tcctgtgtgc
     2281 tgcgccggct ctcgccgagt cgacgtggag ccgccgggtc gcgccgtggc cggagtcagc
     2341 gaacggcccc caccgtggcc atggtggaga ccgttcgctt tcttccctca ggcgttgagg
     2401 cgctctttga gaccgtcgag ttcggcctgc agcggggcgg gaagcttgtc gccgaacttg
     2461 gcgaaccact cctcgatcag cggcagctcc ttgagccagt cctccgtgtt gaccgcgatg
     2521 gccttggcga cctgctcgtc ggtcatgttc aggccggtgg tgtcgatggc gcccggcgca
     2581 gggactagtc cgaccggggt ctcgaccgcg tccgcgtccc cgtcgagacg ctcgacgacc
     2641 cacttcagca cgcggctgtt gtcgccgaag cccggccaca gccaggtgcc gtcctcgtcc
     2701 ttgcggaacc agttgacctg gaagatccgc ggcagcttgc tggcgtcggc ctgcttgccg
     2761 atctccagcc agtggttcat gtagtcgccg gcgtggtagc cgatgaacgg cagcatcgcc
     2821 atcgggtcgc ggcgcacgac gccgaccgcg ccggtcgcgg cggccgtcgt ctccgaggac
     2881 agcgtggcgc cgacgaaggt gccgtgggtc cagtcgcgcg attcgtagac cagcgggacg
     2941 gtcgtggcgc ggcggccacc gaagaggatc gcgtcgatgg gtacaccgtc ggggttgtcg
     3001 tattccggcg cgatcatcgg gcactgcttc gccggggtgg tgaagcggct gttcggatgg
     3061 ctggagacga catcggactc gggcgtccag tcgttgccct tccagtcgat caggtgctcg
     3121 ggcttgtccg gggtcatacc ctcccaccac acatcgccgt cgtcggtgcg ggcgacattg
     3181 gtgaagatcg agttgccctt ctcaatggtg cgcatggcca tcgggttggt gttcatgccg
     3241 gtgccgggcg cgacgccgaa cagaccgaac tcggggttga cggcatacag ccggccgtcg
     3301 gggccgaagc gcatccaggc gatgtcgtcg ccgatcatct cggccttcca gccctcgatc
     3361 gtcggctcga gcatcgcgag gttggtcttg ccgcactggc tcgggaaggc gccggcgaca
     3421 tagtggaccg agcccttggg ggaggtcagc ttgaggatga gcatgtgctc ggccatccag
     3481 ccgtcgtcgc gggccatggc cgaggcgatg cgcagggcgt agcacttctt gcccagcagg
     3541 gcgttgccgc cgtagccact gccatacgac cagatctcgc gggtctcggg gaagtgggcg
     3601 atgtacttgg tgtcattgca cggccacggg acatccttct caccgtccgc gagcggtgcg
     3661 ccgacggagt gcaggccctt gacgaagtcg gcgtccaact cctccatctt cgccagcacc
     3721 tcggagccga tgcgggccat gatcttcatg ttcgtcacga cataggcgga gtcggtgatc
     3781 tcgacgccga acatcgggac ggtcgcgtcc aggtgaccca tgacgaaggg gatgacatac
     3841 atcgtccggc ccttcatgca gcccttgtag agctcggtca tggtggcctt cagctcggca
     3901 gggtcgatcc agttgttggt cgggccggcg tcctcctcct tctcggacgc gatataggtg
     3961 cggtcctcga cacgggcgac atcgctcggg tcgctggcac accagtagga gttgggcttc
     4021 ttctcgtcgt tgagcttgac gaaggtgccg gaggcgacga gctcctggtt gaggccttcg
     4081 atctcctcgt cggtgccggt gatccagacg accttgtcgg gggtcgtcag ggcggccacc
     4141 tcgtcgaccc acgcctgcaa ggcggtattg gtcgttccgg tgctgtcagt catgtgcttc
     4201 ctccgtccgg tgggccccca aggccccagt gcttcgcgcc cgcgcgaccg gttggcagcg
     4261 ctgctgacag gcgcgacaac tgcttccgac aacgctatcg gcctcgtcca ggggggcttc
     4321 tcgtgaaagc cttttcaaac ctgtggaact actcacaggc gttgggtatg ccgcccgctc
     4381 acctcctcgt gttgcaccag gtggtgccgc gtggccgggt gacgacgacg gccgtccggc
     4441 cggatcgcct acggttgagg ccatgacgtc ccggcccggc gacggcccgg caccggctcg
     4501 gccggtccgc ccgcggtggc tgcagccgcc tacgtggcgc ggcgaggcgc cgaccgagcc
     4561 gctgccggtc cgcgcgtccc tgcggcgcac tccctatcgc aatccgcgcg tgcgccggac
     4621 tcaggaggag cgggtcgcga tggacctggc cctcaacgtg ggcgaggtgt tgctgcgttg
     4681 cggggcgagc gcggcgcaga cccacaccag cgtggtcgcg gtgtcgcttg ccgccgggct
     4741 ggaacgaatc gacgtcgaca tcaccctcca gtccttgctg atgcaggtgc agaccgccga
     4801 cggggaggtg gcgacgcggc tgcgcgtcgt cggcccgccg cgctgggact tcgggcgtct
     4861 cgccgcggtc cacgcactcg tcgatgacct tgtctcggga gaggtgaccg tcgcccaggc
     4921 ggtcgagcgc atgcaggaga tccaccgcgc cccacggacc tggtcatcgt gggtcgtcgc
     4981 tctcgccacc ggactccttg cggcgggtgt cgcggtggtg ctcggggccg gggtgctcgc
     5041 cgcggtgctc tgcatcggct cggcgatcct cgtcgaccgc ctcgtcggca ccttgtccgg
     5101 gctcggcatc ccggacttct atgcctctgc cgcaggcggt ttcgtcgcga ccgcgatcgc
     5161 ctatggcgcc tacgcgctgg gctatgtggc gttttggtcg atcaccccgg cggacttcgc
     5221 cgcgatggtc gccggcggca tcatcgcgct gttgcccgcg cgctcgatcg cctcggcgat
     5281 ggaagacgtc gtgaccggat atccggtgac cggcacctcg cggatcttct cggtcctgtt
     5341 tcacaccctg ggcctgatca tcggggtggg ctcggccttg ccgttcagtg tgtgggtcgc
     5401 cacgcgcgcc ggtcagtcgg tcgcgccgcc cgcggtggag cggctggcct cgaccaccgt
     5461 gggcttgccg gtcgcgcttg ccggtgccgc agcgatcggg atcttcggtg ccatcacgat
     5521 gcagagccgc cggcgctacg tgctgccggc gggcggcctc accgtggtcg gcgttctggt
     5581 gacgcgcctg ctcaccagcg atgtcggggt cgggcgggtc acggccgtgg ggctgacggc
     5641 gatcatcatc ggcttcctcg cgcgcgtggt ggcattgcgg atgggggcgc ccgcgctggt
     5701 cctcgccgtg cccgggtcct tcggcctgct gccgggactg gcgatcttcg tcggcctcta
     5761 ccggctgctg gtgacggcgg gcggtgacct cagcggcagt ctgacgatcc aggtcggtgc
     5821 ccaggaactc atctccgcgg tcggtgtcct gatcgcgatc gcgaccggca acaccctcgg
     5881 tgacctgctc gcctcgccgc tcgaccaccg tgcgtcgatg cggcgcaccg aggcccccga
     5941 cggggctggc gagcgggggt agtgtcccta tgctccttgt cgagcggcga ggatggttgg
     6001 ggtggggttc ggtaggctcg tggttgcgga gcatggcgtg gcagctcgag gtgagacacc
     6061 tcggcggtcc cccgcagctg cttgcaattg aatcgacggt gccagaacga tgctgggatg
     6121 agattcgaac agcccacggt cgagctgctc ctggaggccg cgcacatgtc gacagtggca
     6181 ggggtaggta tgaggcccgc cgagatcgag agggagatcg catccgggcg cctacaaccg
     6241 aagtggtccc aggtcgccat cgacgaggac gagtcactcg ttggacgtgc cctttggtgg
     6301 ggtcgagacg ctcaggtccc gctggccctc gacgtctggg atgctcgccg cgaccatccc
     6361 gacaccgaga ggatcctcat cgaactcctg gaacatggtc atgccgcgct ggggtccgac
     6421 ggcgtagaca tcccgctgcc gcacacgatg cgtatcccca ccacctggcg cgacatcgag
     6481 gcggttcact gcgatgtcgc tgtcaagacg aacgccgcag ccgctgcggg cctcgttcgg
     6541 gtcaacgaac gtcgccagtt ccaatgggat caaggcgccc gagctacaag gcgtgcgcac
     6601 cgcctccgct tcgagacggc ggacgacgac gcgttccttc acctatttgc gcgggccacc
     6661 cgcggctcac tggatgtcat gacccgacgc gagctggcta cggccgccgc cgaaacggtg
     6721 gccgttgacg aggtcgctta ctaccggtcc tgtcccggcg accgcggctg gtggcgtgtt
     6781 gcgtacgaca gtcgcggcaa cgtggtcggc atggcgatcc cgtccgccac gccgaccagc
     6841 cgcaatatcg gttacctcgc ggtccttccc gagcaccggg ggcatgggta cgttgatgat
     6901 cttctggcgt acatcactga cttccacatg ggtgatgggg catcccggat caccgccacc
     6961 accgacgccg tgaacacgcc gatgatcgaa gctttcaagc gagccggata tcaatgcgtc
     7021 gagacgcgta tcgacctcga acgctaggca cgttgtctgc cccagaacgc ggatgccggt
     7081 atccggtgag gcgcgcggcg aaggggctcc agcctgcgcg tcggtgatgt tgtcggccct
     7141 gcggactgcc ttctttcagt ggggctgcgg gcgttgaccc ggccgactct caccagagct
     7201 gagcagtcct acagcgcctt ggcgcgatgc tcgggcggct ggtcgtcttc gcccgtgcgg
     7261 cgggtgaaga agctgaccgc gccgtcggcc tccagcaccg ccaggtcgat ctcctcgagg
     7321 tcacggatcc cgtgttcgcg ggcggcggcc agcaggttgg ccatcgagac ccgctcggct
     7381 cgcatggccg tctcgtccgg ccggccgtcc tgaaggagga tcgtcggctc cccctcgatg
     7441 atcttgtcgg cgccggggaa gcgccacgtc agcacggaca atccgaccgc caggagggtc
     7501 acgaggccga ttgcgacgag ggcgctgacg agggagtagt cctcctggag gatgccctgg
     7561 gtgaccacgc cgccgagaac gatgatcgtc accaggtcga acgcgtccat ctgggccacc
     7621 tgccgtttgc cggccacccg cagcacgagc cacagggcga ggaagatgac gacggtgcgg
     7681 agaatcagtt gcatgtcgat cgccgcctaa ggccagaaga cggtgtggaa ggtgacggta
     7741 gcggcatcgc gatccccgtc cagcaccgtc aggtgatagt cgtcgctcga atagccctgg
     7801 ttggggccgg tgcgggcatc gagagacacc tcgaggctgt cacccacggg cgggtcgaat
     7861 tcgtagacca cctgccggcc gtcgttggct tcggcggacg gcaccgggta ccagttctgg
     7921 aagtcgagcc ggtcgacgaa ggcctggtcg agccggacga cgacggggtc gtcaccgaac
     7981 ccgcctgcct tggtcaccgt gaagctgaag ggcgccacga tgctggagcg cgtggcctgc
     8041 ggatagtgga ctgtcagttc ataacccgac ccgctggccg tgccggtcgt ctcccgcggg
     8101 ccgaatacgc cgaatgcggc cgccacgacc gggatcgcga agagcgccag cccgatgcgg
     8161 cgcatgttgc gggcgcgcgt cgcctccgac ccggtcatcg cctcagggag ggtctggatc
     8221 gccacgcggg cagcctaggt cacccggccc ccgcgcgccc ctgcggagcc cgggggcaaa
     8281 ctcaatttcg ccctgccgga ccgagccggt atcgttatcc gtcggtcgcc tccgcggcca
     8341 ctgcgcccgt agctcaacgg atagagcatc tgactacgga tcagaaggtt agggg
//
LOCUS       DAOPJG010000151         8277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9168_length_8277_cov_7.481391, whole genome shotgun sequence.
ACCESSION   DAOPJG010000151 DAOPJG010000000
VERSION     DAOPJG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9168_length_8277_cov_7.481391"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKU75_12540"
     CDS             complement(<1..412)
                     /locus_tag="PKU75_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPF81753.1"
                     /translation="MRLLIVDDERDLAQDLAALLRAEGWVVDIAHDGHTGLAFAQFGE
                     DDVLVLDIMLPGLSGYAILKRVRAQDDRTAIVMLSAKDGEYDQSDAFELGADDYVTKP
                     FSAVVLAARLQAVLRRSGVPRSVALECGDLVVETT"
     gene            487..1005
                     /locus_tag="PKU75_12545"
     CDS             487..1005
                     /locus_tag="PKU75_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81754.1"
                     /translation="MLDTIAGLPVHVLVIHAVVVIAPLTALAAIWYAVRPASRPGLTW
                     PLTAGALVTGVTGLIAGSSGEALERRVTAANAGDAAAIKLVHDHAEAGDLARIFCLGL
                     MVVVLAAIFALLPPAGAPRFGAGLANVTAALLVLAALATTGSVILTGHSGAKAAWADQ
                     VTATNGVSGGGD"
     gene            complement(1042..2130)
                     /gene="amrS"
                     /locus_tag="PKU75_12550"
     CDS             complement(1042..2130)
                     /gene="amrS"
                     /locus_tag="PKU75_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPF81755.1"
                     /translation="MTGGEAGAAYPARYWHRVDDERIQCDLCPRECRLHDGQRGFCFV
                     RMASEGRLVLTTYGRSSGFCIDPIEKKPLAHFYPGTSVLSFGTAGCNLGCRFCQNWDI
                     TKSREMDRLMDQAAPEQLATAALDHGCRSVAFTYNDPVIFAEYALDVAQACRSRGVAT
                     VAVTAGYVNPGAREDFFAGMDAANVDLKGFTEEFYVRQTGAHLATVLDTLTYLKHETD
                     VWFELTTLLIPGHNDSDAEITAMCRWIREELGPDVPQHFSAFHPDHRMRDVDRTPAQT
                     LVRARRIALDEGLHFVYTGNVVNRDGEVTACPTCGTRLIERDWYDLVAYRLTADGRCP
                     GCRTRLPGRFGPTAGNFGRKRIPVRLGS"
     gene            complement(2127..2699)
                     /gene="amrA"
                     /locus_tag="PKU75_12555"
     CDS             complement(2127..2699)
                     /gene="amrA"
                     /locus_tag="PKU75_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPF81756.1"
                     /translation="MLIALARDAIVRELGMAATGAADAVPGGAVDRLPEALERALEEP
                     GACFVTLHTRGRLHGCIGSIEPRRSLREDVESNARSAAFRDRRFPPLTREDLGETTIE
                     VSLLSPMEPVAVSGEAEALRALRPGVDGVLFEAGGHRATFLPQVWASLPDPRAFLQQL
                     RRKAGLPQNYWSDDVILHRYTVTAFDEAAS"
     gene            complement(2816..3607)
                     /gene="amrB"
                     /locus_tag="PKU75_12560"
     CDS             complement(2816..3607)
                     /gene="amrB"
                     /locus_tag="PKU75_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPF81757.1"
                     /translation="MRAPAVAGYFYPAEPEVLRAQVTRFLGDVGPGSGPVPKAVIAPH
                     AGYVYSGSTAALAYAALAPARDRVARVVLLGPTHRVAVDGLALPEAEELATPLGSLHA
                     AVPTADERAALPQIVDRADVHAQEHSLEVHLPFLQVLMPRAEVVPFAVGRARPGTVAD
                     VLERLWGGPETVIVVSSDLSHYLSYDEARRRDAQTIAAITDLRGPIDHEQACGATPVN
                     GLLEVARRRALTARLLGACNSGDTAGDKRRVVGYAAVAFDEPEPS"
     gene            complement(3654..4163)
                     /locus_tag="PKU75_12565"
     CDS             complement(3654..4163)
                     /locus_tag="PKU75_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPF81758.1"
                     /translation="MHTLLLTVIGDDRAGLVKALADTVGEHGGNWERSRLAELAGKFA
                     GIVEVSVPDEQADELRAALAGHAGLLEIDVHEGRAEEADAGPVVRLEVLGNDRPGIVR
                     EVSGALAAHGLSVDELSTETRDAPMAGGQLFEAKITAHAPPGTDLAAVRAALEALAQE
                     IQVDVTVDA"
     gene            4208..4807
                     /locus_tag="PKU75_12570"
     CDS             4208..4807
                     /locus_tag="PKU75_12570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF81759.1"
                     /translation="MAKPTRWVTETDDGHSEWYIERFRTMAAEGVDLVGEARLVDAMV
                     PRGARILDAGCGPGRHAGYLHAVGHHVIGVDADPALIAAAREDYPGPRYEVADLSELD
                     LAALGESEPLDAILVAGNVMVFLAPDTEIEVLRRLGAHLAPDGFVITGFHTTRDLALT
                     DFDAAVAAADLRIEHRFATWDLRAWHGDADFAVTVLRHP"
     gene            complement(5470..5991)
                     /locus_tag="PKU75_12575"
                     /pseudo
     CDS             complement(5470..5991)
                     /locus_tag="PKU75_12575"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
     gene            complement(6463..7128)
                     /locus_tag="PKU75_12580"
     CDS             complement(6463..7128)
                     /locus_tag="PKU75_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPF81760.1"
                     /translation="MGAGSFEGFSRRALDFYAEVERENSREWWLAHKPLYEADVRAPI
                     TALVGALEPDFGVAHVFRPNRDVRFAADKAPYKTHQGAYCATADKAYYYVQIDADGLL
                     VGAGGYRFAADQLGRYRTAVSDDLAGPDLERILSASRADGLEVGGEQLKSRPRGVAAD
                     HPRLELLRHKSIHLSRGIGDPDWVFGPEALPRIRAEWERLRAFTGWLDQHVGATEIDD
                     RFS"
     gene            complement(7128..7787)
                     /locus_tag="PKU75_12585"
     CDS             complement(7128..7787)
                     /locus_tag="PKU75_12585"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HPF81761.1"
                     /translation="MTPARPHVRGELSWITSGPAYCVEVSALEVLEEFLDAHGYTVVA
                     LDGEQMTNRATTFDELRRAFALPPWCGDNWDAVNDCLGEFVHAHDGQLVAIVWCNQDV
                     AAANAPATSAEVGWALLEVVLGRMPSLGPNCPWRIHADLFLTGQGEDYDRPPPKRLPL
                     PGPVECPVCGEMEGVPILRGLPMPEAQEAAREHRAVLRGCIVSPEDDHWFCRACAWEW
                     R"
     gene            complement(7784..>8277)
                     /locus_tag="PKU75_12590"
     CDS             complement(7784..>8277)
                     /locus_tag="PKU75_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPF81762.1"
                     /translation="MTEFERKFVVTDPGPATAWSYEELAQGYLFTRHGYALRVRSATA
                     PDGARVCTMTLKGPRSGLGRPEAEWEIPAADADALLAEATGSVAKRRHTLFVDDLMWT
                     VDIFTGANAPLVLAEVEGPEASVAAAEIPPWCGQEVTTDPRYTNAALSAQPYGTWAAE
                     GPV"
BASE COUNT         1227 a         2933 c         2872 g         1245 t
ORIGIN      
        1 tggtcgtctc gacgaccagg tcgccgcatt ccagggcgac ggatcgcgga acgccgctgc
       61 gccgcaacac cgcctgcagg cgcgcggcga gcaccaccgc cgagaacggc ttggtcacat
      121 agtcgtcggc acccagctcg aaggcatcgg actggtcgta ttcgccgtcc ttggcgctca
      181 gcatgacgat ggccgtgcga tcgtcctgcg cccgcacgcg tttgaggatc gcgtaacccg
      241 acaggcccgg cagcatgatg tcgagcacca gcacgtcgtc ctcaccgaac tgcgcaaagg
      301 ccaaaccggt gtgaccgtcg tgcgcgatgt cgacgaccca gccctcggcg cgcagcaggg
      361 cagcgagatc ctgcgcgaga tcgcgctcgt cgtcgacgat cagcaggcgc acggggtcac
      421 ctctcagggt cctctcagcg cggcggacat acggtcgaag cactctccca cagaagggct
      481 cagctcgtgc tcgacactat cgccggactc cccgtccacg tcctcgtcat ccacgccgtc
      541 gtcgtgatcg cgccgctgac cgcgctggcg gcgatctggt atgccgtgcg cccggcctct
      601 cgtcccggcc tgacgtggcc gttgaccgca ggcgccctcg tcaccggcgt gaccgggctg
      661 atcgccgggt ccagcggcga ggcgctggaa cgccgcgtga ctgcggcgaa tgcgggcgac
      721 gccgccgcca tcaagctcgt tcacgaccac gccgaggccg gtgatctggc gcggatcttc
      781 tgcctcggtc tcatggtcgt cgtgctggcg gccatcttcg ccctgctgcc gccggccggc
      841 gctccccgct tcggcgcggg cctggccaat gtgaccgcgg ccctgctcgt tctcgcggcc
      901 ctggcgacga ccggctcggt catcctcacc gggcacagcg gcgccaaggc cgcctgggcc
      961 gaccaggtca cagcgaccaa tggcgtgtcc ggaggcgggg actgacgccc ttcggcacga
     1021 ccccccggac gccggtccgc ctcagctccc gaggcggacc ggtatgcgtt tgcgaccgaa
     1081 attcccggcg gtcggtccga accggccggg aagccgcgtt cggcatcccg ggcagcggcc
     1141 gtcggctgtc aggcggtagg cgaccaggtc gtaccagtcg cgctcgatca gccgggtgcc
     1201 gcaggtcggg cacgcggtga cctcgccgtc gcggttgacg acattgccgg tgtagacgaa
     1261 gtgcaggcct tcgtcgagcg cgatccgccg tgcccgcacg agggtctgcg cgggggtgcg
     1321 gtcgacatcg cgcatccggt ggtcgggatg gaaagccgag aagtgttgcg gcacatcggg
     1381 tcccagctcc tcacggatcc accggcacat cgcggtgatc tcggcgtcgg agtcgttgtg
     1441 gccggggatg agcaaggtcg tcagctcgaa ccacacgtcc gtctcgtgtt tcagataggt
     1501 caacgtgtcc agcacggtcg cgaggtgggc gccggtctgg cggacgtaga actcctcggt
     1561 gaaacccttg aggtcgacat tggccgcgtc catgccggcg aagaagtcct cccgagcccc
     1621 gggattgacg tacccggcgg tcacggcgac ggtggcgacg ccccgggagc ggcaggcctg
     1681 ggcgacgtcg agggcgtatt cggcgaagat gaccgggtcg ttgtaggtga acgccacact
     1741 gcggcagccg tggtcgagcg ccgccgtcgc caactgctcc ggtgcggcct gatccatcag
     1801 ccggtccatc tcgcgcgact tggtgatatc ccagttctgg cagaagcgac agccgagatt
     1861 gcacccggcg gtgccgaacg acagcacgct ggtcccggga tagaagtggg cgagtggctt
     1921 cttctcgatc gggtcgatac agaagccgga actgcggccg taggtcgtca ggaccagccg
     1981 gccctcggac gccatgcgca cgaagcagaa gccgcgctgc ccgtcgtgga gccggcactc
     2041 acgcgggcac aggtcgcact ggatgcgttc gtcgtcgacg cggtgccagt accgcgccgg
     2101 gtatgccgcg cctgcctcgc cgccggtcat gacgcggcct cgtcgaaggc ggtcaccgtg
     2161 tagcggtgca gtatcacatc gtcactccaa tagttctgcg ggagaccggc tttgcggcgc
     2221 agctgctgca ggaaggcacg cgggtccggc aggctcgccc agacctgcgg caggaaggtc
     2281 gcgcggtggc cgccggcctc gaacagcacg ccgtcgacac cgggccgcag ggcacgcagg
     2341 gcctcggcct caccgctgac cgccaccggc tccatcggcg acagcagcga gacctcgatc
     2401 gtggtctcgc ccaggtcttc tcgggtcagc ggcgggaagc gccggtcccg gaacgccgcg
     2461 gagcgggcgt tgctctcgac gtcctcccga aggctgcggc gcggttcgat ggacccgatg
     2521 cacccgtgca accgcccgcg ggtatgcagc gtgacgaagc acgctcccgg ctcctccagc
     2581 gcccgctcga gcgcctccgg gagccggtcg accgccccgc ccggcaccgc gtccgccgcc
     2641 cccgtcgccg ccatccccaa ctcccgcacg atcgcgtcgc gcgccagcgc gatcagcacc
     2701 tccccccgtc gagatgtcga ttcgcccccg tcgagatgcc gattcgaccc cgtcgagatc
     2761 gcccttcctg cgggtcggga tggcgccgac cggccgtcgc cggaacgtcc gcgcgtcatg
     2821 acggctccgg ttcgtcgaag gcgaccgcgg cataccccac gacccggcgt ttgtcgccgg
     2881 cggtatcgcc ggagttgcac gcgccgagga gccgggcggt cagcgcgcgg cggcgagcga
     2941 cctcgagcag tccgttcacc ggcgtggcgc cgcaggcctg ctcatggtcg atcggtccgc
     3001 gcaggtcggt gatggccgcg atcgtctggg catcgcggcg gcgggcctcg tcatacgaca
     3061 ggtagtgcga gagatcggaa ctgacgacga tgacggtctc cggtccgccc cacagccgct
     3121 cgagaacgtc cgccaccgtt cctggcctgg cccgcccgac ggcgaagggc acgacctcgg
     3181 cccgcggcat gagcacctgc aggaagggca ggtgcacttc cagcgagtgc tcctgggcat
     3241 ggacatcggc gcgatcgacg atctgcggca gggctgcccg ttcgtccgct gtcggcaccg
     3301 ccgcgtgcag ggaccccagc ggcgtcgcca actcctcggc ctccgggagg gcgagaccgt
     3361 cgaccgcgac ccggtgcgtc ggcccgagca gcaccacgcg ggcgacccgg tctcgggcgg
     3421 gcgccaacgc ggcatacgcc agggccgccg tcgatcccga gtagacataa cccgcgtgcg
     3481 gggcgatgac ggccttcggc accggcccgg agcccgggcc tacgtcgccg aggaaccggg
     3541 tgacctgcgc acgcaggacc tccggctccg ccggatagaa gtagcccgcc acggcgggcg
     3601 cccgcactct gcccactcct ccagtctgcc ccggcagcgc cgggatcggg gcgtcaggcg
     3661 tcgacggtca cgtcgacctg gatctcctgg gcgagggctt ccagggcggc gcgcacggcg
     3721 gcgaggtcgg tgccgggggg cgcgtgagcg gtgatcttcg cctcgaagag ctggcccccg
     3781 gccatcgggg cgtcacgggt ttcggtcgac aactcatcga cgctgagtcc gtgtgcggcc
     3841 agcgcaccgg agacctcgcg cacgatgcct gggcggtcgt tgccaagcac ctcgagccgc
     3901 acgaccggac cggcgtccgc ctcctcggct cggccttcgt ggacgtcgat ctcgagcagg
     3961 ccagcgtgcc ccgcgagcgc cgcccgcagt tcgtcggcct gctcgtcggg aacgctgacc
     4021 tcgacgatgc cggcgaactt gcccgccaac tcggcgaggc ggctgcgttc ccagttgccg
     4081 ccgtgttcgc cgaccgtgtc ggccagggcc ttgacgagcc ccgcgcggtc gtcaccgatg
     4141 acggtcaaca ggagagtgtg cacgactggc atcgtaaccg gcgggcgcac ggcataccgt
     4201 ggcgtgcatg gcgaagccga cgcgttgggt gaccgagacc gacgacggac acagtgagtg
     4261 gtacatcgag cgcttccgga ccatggccgc cgagggcgtc gacctcgtcg gcgaggcgcg
     4321 gctggtcgat gcgatggtgc cgcgcggggc gcgcatcctc gacgccgggt gcgggccggg
     4381 ccggcacgcc ggttatctgc atgccgtggg tcatcacgtc atcggggtgg acgccgatcc
     4441 cgcgttgatc gcggcggcgc gtgaggatta ccccgggccg cggtatgagg tggccgacct
     4501 cagcgaactg gacctcgccg cgctcggaga gagcgaacca ctcgacgcga tcctcgtcgc
     4561 cggcaatgtc atggtcttcc tcgccccgga caccgagatc gaggtgctgc ggcggctcgg
     4621 tgcgcatctg gcgccggacg gcttcgtcat caccggattc cacacgacgc gcgatcttgc
     4681 cctgaccgac ttcgacgcgg cggtcgcggc tgccgacctg cgtatcgagc accgcttcgc
     4741 cacctgggac ctacgggcct ggcacggcga cgccgacttc gctgtgacgg tgctccggca
     4801 tccatgacct ctccacggcg gacccctggc gggatcgggt ggaccccgtg gcgggtcgtg
     4861 tcgggcctgg gctttgtcag cctcgccgcg gacatggtct atgaaggcgc tcgctccgtc
     4921 tacggcccgt tgctgggggc tctcggcgca agcgccgtca ccgtgggcct ggtgtccggt
     4981 gccggtgagg cggccgcgct cgtgctccgg ttggtcttcg ggccgctggc cgaccgcact
     5041 cgcggctact ggccactgac cgtcgccggc tatgccctca cagccgtctg cgtgcctttg
     5101 ctggccctgg ctccccgcgt cggtgcggcc ggactggcct ttgccgtcat cctgatcctg
     5161 ttggaacgca ccggcaaggc cgtgcgatcg ccggccaaga gcgcgctgct ggccgatgct
     5221 gccacgtcgg tcagccgcgg cgcgggttcg gcgtccacaa ggcgctcgac cagccgccct
     5281 gcgcgcgtat gcccagcccg ggccgcgcag gaggaacccg aagcggtcgg cccagttgcc
     5341 ggccctggcg atatcgcggg cgatgtcccg ccattcgtgc gtcgcgatcc ggaccgggtt
     5401 gacggtgtcg atattgcgcg tcagcccata gaccgcacgc tctgtttccg gctcgaacgt
     5461 gccgaagagc cggtcccaga tgatgaggat cgacccgtga ttgcgatcca gatagcggcg
     5521 gttcgagccg tgatgcaccc ggtggtgcga cggagtgttc agcaccttct cgagccagcc
     5581 gatcgagcgc accacctcgg tgtggatcca gaactgatag atcaagttga gggcacgggc
     5641 gttctcgatg agtgtcggcc gcaccccggc caacgccagc acgccatacg gcaccgtcca
     5701 cgtcacgcct tcggcgacgg gctgccgcag ggcggtggag aggttgtagt gctcactgct
     5761 gtgatgcacc acgtgcaccg cccacagcca gcggctctcg tgggagagcc ggtggttcca
     5821 gtaatagatg aagtcccagc cgaacaacgc caaaaggctt gccgcccaac cggtcccgag
     5881 gtcgcgtcgg ccgagcgcaa agagacgttt cccgctcgtc tgcgccgccc acgtcgtggc
     5941 agcagcgagt gcggtcgccc cgacggcagc gaccgctgtc acgccggtca tcgtgggatt
     6001 cgcgggcagc gccggcggtg gtgcgctcga cccgtatcga gaggtctcgg agacgacgcc
     6061 cgccgcgggc aatcggccgt atgccgtgcg ccggcgcacg atgtccgccg cggtcgtcgc
     6121 gacgccggca gcgacggcgg tgccgatcag cgccttcccc caccggccga cgccaggtgt
     6181 cagggggtcg agcacgcgct tggcaacgtg cggcgcaacg aggctgccca ggcccatcgt
     6241 gaggctggcg atggtgtcgg acagttcgta atccccagcg cgaggggtgc cctccggggc
     6301 gggatgcctt ctcagccaga catattcggc acccatcgcg ccgatgtagg cagggatcgc
     6361 gaggactgtc aggtcgaagc gagccatcgt ccgtgtccct atctgtggct gtcggttcga
     6421 ggtcctcgcg acatgccgca cagcgaggac acatcaggag gtctaggaga accggtcgtc
     6481 gatctcggtc gcgccgacat gctggtcgag ccagccggtg aaggcccgga gtcgctccca
     6541 ctcggcccgg atccgcggca acgcctcggg accgaacacc cagtccgggt ccccgatccc
     6601 ccgtgataga tgaatgcttt tgtgccgcaa caactccagc cggggatggt cagcggcgac
     6661 gccacgggga cgcgacttca actgctcgcc gcccacctcg aggccgtcag ctcgcgaggc
     6721 agagaggatc cgttccaggt ccggtccggc gagatcgtca ctgaccgccg tgcggtagcg
     6781 cccgagctga tccgccgcga agcggtagcc gccggccccg acgagcagcc cgtccgcgtc
     6841 gatctggacg tagtagtagg ccttgtcggc ggtcgcacaa taggcaccct gatgggtctt
     6901 gtagggcgct ttgtctgccg cgaagcgcac atcacggttc ggccgaaaca catgggccac
     6961 accaaaatcc ggctccagcg caccaacgag tgcggtgatc ggcgccctca cgtccgcctc
     7021 atagaggggc ttgtgcgcca gccaccattc gcgggagttc tccctctcca cctcggcata
     7081 gaagtccagc gcccgccggg aaaaaccctc gaaggatcca gcccccatca acgccactcc
     7141 catgcgcagg cgcggcagaa ccagtggtca tcctcgggtg agacgatgca cccgcgcaag
     7201 acggcgcggt gttctcgggc cgcctcctgc gcctcgggca tcggcaagcc acgcaggatg
     7261 ggcacgccct ccatctcacc acagaccggg cactcgaccg gcccggggag cggaagccgt
     7321 ttcggcggcg gacggtcata gtcctcaccc tgaccggtga ggaacaggtc ggcgtgaatc
     7381 cgccacgggc agttcgggcc gaggctcggc atccggccca gcacgacttc cagcagcgcc
     7441 cacccgactt ccgcgctggt ggcgggcgcg ttggcggccg ccacatcctg gttgcaccac
     7501 acgatcgcca ccaactgccc gtcgtgagcg tgcacgaact cccccaagca gtcgttgacc
     7561 gcatcccagt tgtcgccgca ccacggcgga agggcgaagg cccggcgcag ttcgtcgaag
     7621 gtcgtggcgc ggttcgtcat ctgctctccg tcgagcgcga caacggtata gccgtgggcg
     7681 tccaggaact cctcgagcac ctccagcgcc gagacctcga cgcaataggc cggccccgag
     7741 gtgatccagg acaactcccc gcgcacgtgg gggcgagcgg gcgtcatacc ggaccctcgg
     7801 ctgcccacgt gccgtagggc tgcgccgaca gggcggcgtt ggtgtagcgg ggatcggtgg
     7861 tgacttcctg gccgcaccag ggcgggatct cggcagccgc gacgctggcc tcgggcccct
     7921 cgacctcggc caggacgaga ggcgcgttgg cgccggtgaa gatgtcgacg gtccacatca
     7981 ggtcgtcgac gaagagggta tgccgccgtt tggcaacgct gcccgtcgcc tccgcgagca
     8041 gggcgtcggc gtccgcggcg gggatctccc actccgcctc cggccggccg agcccggagc
     8101 gggggccctt gagcgtcatc gtgcacaccc gtgcgccgtc gggcgcggtg gccgagcgga
     8161 cccgcagggc gtagccgtgg cgggtgaaga gatagccctg ggcgagctct tcgtagctcc
     8221 aggccgtcgc cggacccgga tcggtgacga cgaacttgcg ctcgaactcg gtcatgc
//
LOCUS       DAOPJG010000152         8225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9252_length_8225_cov_7.386169, whole genome shotgun sequence.
ACCESSION   DAOPJG010000152 DAOPJG010000000
VERSION     DAOPJG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9252_length_8225_cov_7.386169"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PKU75_12595"
     CDS             <1..340
                     /locus_tag="PKU75_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPF81763.1"
                     /translation="EFDPETESPRQAAEREWAEETGLPVPQGDWFEMGTVKQSGGKVV
                     HAFAVLVTEPFDVPAGAGNTVTTQWPPRSGTSITFPEIDRTQWCTLQEAGPRVVAAQE
                     AFLERLELPQ"
     gene            351..1232
                     /locus_tag="PKU75_12600"
     CDS             351..1232
                     /locus_tag="PKU75_12600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPF81764.1"
                     /translation="MKMPRRDLDLAAVLHQLDPGLGQAWIEELGEGWDNVAYAVRRPV
                     GDDLLLRISKFADPEERARTLHTDVALLDFVAAHATLATNRVLAADPQTGALLLTPVP
                     GRSADLLSPADPARAARELGVFLARLHTAEPPPGLVEPGRDLGLWRREAASAWGEARA
                     LCAEDVQREVDSFLAAPLPPRPERMVFCHNDLGDEHVIVDPASGQISGVIDWSDAVHD
                     DPARDFALLLFDFGDAFFEGAWAAYGGTDEQLPVRARWYGARAGILGVCDRARRGDPG
                     LTASLARLRSVLARPAT"
     gene            1373..2689
                     /locus_tag="PKU75_12605"
     CDS             1373..2689
                     /locus_tag="PKU75_12605"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HPF81765.1"
                     /translation="MKRSMISATAIVALAGAFVPAGAAQSASAAGQAIHVAVGGSDRA
                     SGTAQAPLATIQAAVDAAVPGTVIKVHAGTYKGEVKIRKSGTPTAPITLTNAGDGEVV
                     VTSDQAPDSCTSHQPSMRRTIKITGGASDWTIRGLTIVHGLLILGEKSTAAFNWHATK
                     VKERNWADRRKVPGRGQRDPSSTHRVIPYLQGVTGATTMASADRIRLYGNKIRSRGIH
                     ASLSNYGVIQGNVISDIICGSGPGVWVMTFSDHWTIADNDISRLSAASTEHYMHEGIR
                     LGTASNYNYVARNSVHDLPGDGRAYNTDVDSSYNLFQFNRANKVAIGFNEQMAGWGNT
                     WEYNAVTNFRVFGYGLRLKDAKLPLPSMDTSTNGVIMRCNRAFKPVGKAKSLGVGAVM
                     NARLNTNAFTTIWFSPASQTYWSRFDNRWNGSTQLPDKDVTPSYCG"
     gene            complement(2716..3735)
                     /locus_tag="PKU75_12610"
     CDS             complement(2716..3735)
                     /locus_tag="PKU75_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81766.1"
                     /translation="MRARARLALVGLAVLIGATSTAVAAPASTALEAVGRGNLLAYTA
                     GIDIDQDGMFAAGTPEEEGTDIWVRDVTGATPPVMLNRPNSVADVTPSFSPDGTKIAW
                     ASRDTPTGTLDILVKTLSTGAVTNLTNSGSVDERWPSWSGDGTRILYNRRTTPTSNLD
                     IWVTNADGTSQRYLAGKIGDGKFYEDCCASFTTDGASIVFASNRTGQFDIYRYDLAGA
                     GSRERAKYLTRLTYAPWYQGTPAVEARGTVVYRNASDHQIYRLDPFRAPAQAPTAVRV
                     PGQVRTPQGTPDGRGLVFGWRPGPRQALDIILTDRFGANPVNLTNTRVITETNPTWQP
                     KPPRP"
     gene            complement(3795..5078)
                     /locus_tag="PKU75_12615"
     CDS             complement(3795..5078)
                     /locus_tag="PKU75_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81767.1"
                     /translation="MPRPYLPALPADSTWDAATGRVVSRHMGRRWLWLLIAAGVCTQT
                     ALNVVRPVLSYKLIALGADGTTIGLVTAAYAVLPLFVALSLGRLTDRTPRLRLLIQVA
                     AVVLGVGGVLAAVSGSVVGVALGSVFLGLGHLVFTISGQAAIARYSRDRDLDLGFGWF
                     TAAYSFGQTIGPLIGGALVGSGSVAQPGRIGAINTALAVGTIVALAAVPVMAIPAVLP
                     ASRSTAGKADSDVRPRASIAQILRVPGVSASMLASLTLLSAMDILIAFLPVVGERAGV
                     SPVVIGWLLAVRGATGILSRSLLPWISARISRQRLLAVSALGAAVGLAISPLVITNWL
                     VSGVALAVGGFFLGLGQPITMTLISTAVPSIWRSQALAVRLMGNRVGQVGFPLVAGLV
                     ATPFGPAGAIWMSCLALVASAAQQLHTGGSGHEPR"
     gene            5077..6312
                     /locus_tag="PKU75_12620"
     CDS             5077..6312
                     /locus_tag="PKU75_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HPF81768.1"
                     /translation="MAHESAPEPTEVPTLDPVEQRVLGSLMEKQVTVPASYPLSLNGL
                     QTACNQATSREPVTDYAASDIETVCRALRDRGLVRIVWAGAGSRVLKYHQRLEEALEL
                     AADEKAVLTVLLLRGEQTAGELKTRTDRLHKFAGREDVEIVLQRLAGRSLPLVQQLPR
                     RPGQHDERWIHLLGPIAGLAPVAAAEPTVDRDVVVALGASARDEKVRAGYDAVASAYA
                     AELGNELAGKPFDRWLLSHVADQAGGGPVADVGCGPGQTTAVLAAAGARVTGFDFSLA
                     MLDEARALHPDVTFKAGDYTRLLRPPAASAWAAITAWYAFVHAAGSELVGILSGLARV
                     LQPGGVLAFCVHVGDEVRHVDELFGAATDLDFVLHDSQEVVDAVSAAGLVDVQWFLRS
                     ALPTEAQTQRLYVVARQPD"
     gene            6351..6917
                     /locus_tag="PKU75_12625"
     CDS             6351..6917
                     /locus_tag="PKU75_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81769.1"
                     /translation="MIAWSLALAVLSLLSNITSTEQLSGAADTWLTIRLTLSEITNSG
                     TAWAGIGILGGWLVRRPGIAAAAGVVATGIAVYAHYGLGHLAGIYDSGIWASNVEWLI
                     APVVVGAPLGLIGALARRRSPWGLLARLIVPLGALAEPWVVSMWPWLSQPLTGWPTRI
                     AETVTAVVLTAFGLVLGTRVLARKPRTR"
     gene            complement(6939..7697)
                     /locus_tag="PKU75_12630"
     CDS             complement(6939..7697)
                     /locus_tag="PKU75_12630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81770.1"
                     /translation="MNARPVTSFGELWAASGEHPMVRAEIPSDQPLDAVVLGNAIAFA
                     RPSHIRGIVLTLLGPEADARILLRDPQLAALLFRERPTGMLSVPARLRADVEEIFRAG
                     PGGDWEWMSTTTPPPAQPAEDDLIEFDATAKEELDTFLAEHNSRTDGQPFRRDGQLWL
                     GARDPAGHVIACGCSEPSAAGIPELSGITVASEQRGHGWGAAVTAALTRRAISEVGMC
                     ALGMYSDNDRAREIYHRLGFVTAQAFSSRVVARR"
     gene            8046..>8225
                     /locus_tag="PKU75_12635"
     CDS             8046..>8225
                     /locus_tag="PKU75_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81771.1"
                     /translation="MTGGVGGRREPGWVPPPDWFDESLFAEPSAYECELAEEQALIEH
                     VSRCADDGIRDYVADP"
BASE COUNT         1306 a         2744 c         2935 g         1240 t
ORIGIN      
        1 cgagttcgac cccgagaccg agtcgccgcg gcaagccgcc gaacgagagt gggctgagga
       61 aacgggactg cctgtgccgc agggtgattg gttcgagatg gggaccgtca aacagtccgg
      121 cggcaaggtc gtccatgcct tcgccgtgct cgtcactgaa cccttcgacg tgcccgccgg
      181 agccggcaac acggtgacga cgcagtggcc gccccggtcc ggcacgagca tcactttccc
      241 ggagatcgac cggacgcaat ggtgcaccct ccaagaagct gggcctcgcg tggtcgccgc
      301 acaggaggca ttcctcgaac ggctggagct gccgcagtga cgaggcatcg atgaagatgc
      361 cgcgccggga cctcgacctg gcggcggtgc ttcaccaact ggaccccggg ctggggcagg
      421 cttggatcga ggagctcggc gagggctggg acaatgttgc gtatgccgtg cgccgccccg
      481 tcggggacga cctactgctg cggatcagca agttcgccga ccccgaggag cgggcacgta
      541 cgctgcacac cgacgtcgcc ctgctcgact tcgtcgcggc gcacgcgaca ctcgccacga
      601 atcgggttct cgccgctgat ccgcagacgg gtgcgctgct gctgacgccg gtgcccggcc
      661 gaagcgccga cctgctgtcg cccgccgacc cggcgcgagc ggcccgtgag ttgggggtct
      721 tcctcgcgcg actgcatacc gccgaaccac ctccggggct ggtcgagccg ggacgcgatc
      781 tggggctttg gcgccgtgaa gcggccagcg cttgggggga ggcccgggcc ttgtgcgcgg
      841 aggatgtgca acgtgaggtc gactccttcc tcgcggcccc tctcccgccg cgccccgagc
      901 ggatggtgtt ctgccacaac gatctcggcg acgagcatgt catcgtcgat cctgccagcg
      961 ggcagatcag cggcgtcatc gactggagcg atgcggtcca cgacgacccg gcccgcgact
     1021 tcgccctgtt gctcttcgac ttcggtgacg ccttcttcga gggggcgtgg gcggcatacg
     1081 gcggcaccga cgagcaactg cccgtgcggg cgcgctggta cggcgcacgc gcggggatcc
     1141 tcggcgtctg cgatcgcgcg cggcggggcg accctggcct gacagcgagt ctcgcgcggc
     1201 tgcgctcagt gctcgcgcgg ccggcgacct gaccggcccg gcggtcacga acccctgacc
     1261 agcgctgact ccgccgctac gaaggcccga accgcaaggg cggatccggt ttgcgtgtaa
     1321 gttcgggttc tgaactgctg actacccccc accagcagca attggagacc tcgtgaagcg
     1381 atcgatgatc tcggcgacgg cgatcgtcgc ccttgccggt gccttcgtcc ccgccggcgc
     1441 cgcccaatcg gcctctgcgg cgggacaagc gatacacgtc gctgtgggtg gttccgatcg
     1501 agcgtccggg acggcacagg cccccctggc cacgatccaa gccgccgtcg acgccgctgt
     1561 gccgggcacg gtgatcaagg tgcacgcagg cacctacaag ggcgaggtga agatccgcaa
     1621 gtccggcacc ccgacggcgc ccatcacctt gaccaacgcc ggcgacgggg aggtcgtcgt
     1681 cacctccgac caggcgccgg attcgtgcac cagccaccag ccgagcatgc ggcgcacgat
     1741 caagatcacg ggcggggcga gcgattggac gattcgcggg ctgacgatcg tccacggcct
     1801 cttgatcctt ggcgagaagt cgaccgccgc cttcaactgg catgcgacga aggtcaagga
     1861 gcgcaactgg gccgaccgtc gcaaggttcc gggccgcggc caacgcgatc cgtcgtcgac
     1921 gcatcgcgtc atcccgtacc tccagggcgt gaccggcgcg acaaccatgg cgtccgccga
     1981 ccggatcagg ttgtacggca acaagatccg aagccgcggc atccacgcct ccctgagcaa
     2041 ctatggcgtg atccagggca atgtcatctc ggacatcatc tgcgggtccg gaccaggggt
     2101 gtgggtcatg acgttcagcg accactggac gatcgcggac aacgacatct cccgactgtc
     2161 cgcagccagc accgagcatt acatgcacga aggaattcgc ctcgggacgg cgagcaacta
     2221 caactacgtg gcacgcaact ctgtccacga cctgccaggt gacgggcggg cctacaacac
     2281 ggacgtcgac tccagctaca acctgttcca gttcaaccgg gccaacaagg tcgccatcgg
     2341 cttcaacgag cagatggccg gatggggcaa cacctgggaa tacaacgcgg tcaccaactt
     2401 ccgcgtcttc ggctacggcc tgcggctgaa ggacgccaag ctgccgctgc cgtcgatgga
     2461 caccagcacc aatggcgtca tcatgcgctg caaccgtgcc ttcaagccgg tcggcaaggc
     2521 gaagtccctc ggtgtgggcg cggtgatgaa cgcccgcctg aacaccaacg cgttcaccac
     2581 catctggttc agcccagcct cacagaccta ctggagccgg ttcgacaacc ggtggaacgg
     2641 cagcactcaa ttgccggaca aggacgtcac tcccagctac tgcgggtgac ccctccgtca
     2701 ccgtggtcga acgcgctacg gccgcggcgg tttcggctgc caggtgggat tggtctccgt
     2761 gatcacgcgc gtattggtca gattgaccgg gttggcgccg aagcggtccg tcaggatgat
     2821 gtccagtgcc tgccggggac cgggtcgcca gccgaacacc agacccctgc catcgggcgt
     2881 gccctgaggc gtgcggacct ggcccggcac gcgcaccgcg gtgggcgcct gcgccggcgc
     2941 gcggaacggg tcgaggcgat agatctgatg gtcggaggca ttgcgataga ccaccgtgcc
     3001 gcgggcctcg acggccgggg tgccctgata ccagggtgcg taggtcaacc gggtcaggta
     3061 cttcgcccgc tccctcgagc ccgctccggc gaggtcgtaa cggtagatgt cgaactggcc
     3121 ggtgcggttg gaggcgaaga cgatcgacgc tccgtcggtg gtgaatgagg cacagcagtc
     3181 ctcgtagaac ttcccgtcgc cgatcttgcc ggcgaggtag cgctggctgg tcccgtcggc
     3241 gttggtgacc cagatgtcca ggttcgacgt cggggtggtg cggcggttgt agaggattcg
     3301 ggtgccgtcg ccggaccacg acggccagcg ctcgtccacc gatccgctgt tcgtcaggtt
     3361 cgtgacggcg ccggtcgaga gggtcttgac caggatgtcg agggtgccgg tcggggtgtc
     3421 ccgcgacgcc cacgcgatct tcgtcccgtc cggggagaag ctcggggtga cgtcggctac
     3481 cgagttcggg cggttgagca tgaccggcgg ggtggccccg gtgacatcgc gcacccagat
     3541 atcggtgccc tcctcctcgg gcgtgccggc cgcgaacatg ccgtcctggt cgatgtcgat
     3601 ccccgcggtg taggcgagca ggttgccgcg gcccaccgct tcgagggcgg tgcttgcggg
     3661 ggccgccacc gcggtgctgg tagccccgat caggacggcg agaccgacca gggcgagacg
     3721 agcacgggca cgcatggtag gcactctaac gcctggtcag agcggcgccg aggcgacgcc
     3781 gaatcccggc acagctagcg gggctcgtgc ccgctgccgc cggtatgcag ttgctgggcc
     3841 gccgaggcga ccagggcgag gcaggacatc cagatcgccc cggccgggcc gaagggagtg
     3901 gcgaccaggc cggccacgag cgggaaaccg acctgaccga cgcgattgcc catcagccgg
     3961 acggccaggg cttgggagcg ccagatgctc ggcaccgccg tcgatatgag cgtcatcgtg
     4021 atgggctggc cgaggccgag gaagaacccg ccgaccgcca gcgcgacacc ggagacgagc
     4081 cagttggtga tcaccagcgg cgagatcgcc agacccacgg cggcgccgag cgcggacacc
     4141 gcgagcaggc gctgacggga gatgcgcgcc gagatccacg gcagcagtga gcgcgacagg
     4201 atacccgtgg cgccccggac cgcgagcagc catccgatga cgaccgggga gacgccggcg
     4261 cgttcgccca ccaccggcaa gaaggcaatg aggatgtcca tcgccgagag cagggtgagg
     4321 gacgcgagca tgctcgccga cactcccggc acacgcagga tctgggcaat ggaagcacgc
     4381 ggccggacat ccgaatccgc ttttcccgca gtcgatctgg acgccggcag cacggccgga
     4441 atcgccatca ccggcacggc cgccagcgcg acaatggtac ccaccgcgag ggcggtgttg
     4501 atggcgccga tccgtcccgg ctgcgcgacc gatcccgaac ccaccagcgc gccgccgatg
     4561 aggggaccga tggtctgccc gaacgagtat gccgccgtga accacccgaa gcccaggtcg
     4621 aggtcgcggt cccgggagta gcgcgcgatg gccgcctgcc cgctgatggt gaagacgagg
     4681 tgtcccaagc caaggaacac cgaacccagc gccacgccca cgaccgagcc ggacaccgcc
     4741 gccagcactc cgccgacacc gagcacgacg gcggccacct ggatcagcag ccgcagccgc
     4801 ggtgtgcggt ccgtcaagcg cccgagggac agcgcaacga aaagggggag cacggcatac
     4861 gccgcggtga ccagaccgat cgtcgtcccg tcggcgccga gcgcgatcag cttgtaggac
     4921 aggaccggcc ggacgacgtt gagagccgtc tgagtgcaga caccggcggc gatcagcaac
     4981 cacagccacc ggcgtcccat gtgccgcgag actacgcggc ccgtcgccgc gtcccacgtg
     5041 gagtcggcgg ggagagcggg caagtagggt cgtggcatgg cccatgagtc cgctccggag
     5101 cccacggagg tgccgaccct cgaccccgtc gagcagcgcg tgctcggctc cttgatggag
     5161 aagcaggtca ccgtgccggc gtcgtatccg ttgtcgctca acggcttgca gacagcctgc
     5221 aatcaggcga cgagccgcga gccggtcacg gactatgccg cctcggacat cgagacagta
     5281 tgccgtgccc tcagggaccg ggggctggtg cggatcgtgt gggcgggcgc gggctcacgg
     5341 gtgctgaagt accaccagcg gctcgaggaa gccctcgaac tggccgcgga cgaaaaggcg
     5401 gtgctgacgg tgctgctgct gcgcggcgag cagacggcgg gtgagctgaa gacgcgcacg
     5461 gaccggctgc acaagttcgc cggccgcgag gatgtcgaga tcgtcctgca gcggctcgcc
     5521 ggccgttccc tgcccctggt gcagcagcta cctcgccgcc cggggcagca cgacgaacgc
     5581 tggatccacc tgctcggccc gatcgccggc ctcgcccccg tggccgcagc ggagccgact
     5641 gtcgatcgtg acgtggtggt ggcgctgggc gcgtcggcgc gcgacgagaa ggtgcgggcc
     5701 ggatacgacg cggtggcgtc ggcatacgca gcagaactcg ggaatgagtt ggcgggcaag
     5761 ccattcgacc gctggctgct gtcgcatgtc gcggatcagg cgggcggtgg cccggtggcg
     5821 gacgtcgggt gcgggccggg acagaccacc gccgtgctgg cggctgccgg ggcgcgggtc
     5881 accggattcg acttctcgct cgccatgctc gacgaggcgc gcgctttgca cccggacgtg
     5941 acattcaagg caggcgatta cacgcgtctg cttcggccac ccgcggcctc ggcgtgggcc
     6001 gcgatcaccg cctggtatgc gttcgtgcac gccgcggggt ccgaattggt cggcattctc
     6061 agcggcctgg cgcgggtcct gcagcccggg ggagtgctgg cgttctgtgt ccacgtgggg
     6121 gacgaggtgc gccatgtcga cgaactcttc ggcgccgcaa cggatctcga cttcgtgttg
     6181 catgacagcc aggaggtcgt cgatgcggtc tccgccgcgg gactcgtcga cgtccagtgg
     6241 ttcctgcgct cggcgttgcc gaccgaggcg cagacccagc ggctgtatgt cgttgcgcgg
     6301 caacccgact gatggccttt gaaggcaccc ggggtcgcgc gtggtgcttg gtgatcgcgt
     6361 ggtctcttgc cctcgccgtc ctctcgctgc tgtccaatat cacgtctacg gagcagctgt
     6421 ccggggcggc agacacctgg ctgaccatcc ggctgacgct gagcgagatc accaattccg
     6481 gcacggcctg ggccggcatc gggatcctcg gtggctggct cgtgcgccga cccgggatcg
     6541 ccgcggccgc gggggtggtt gcgaccggca tcgccgtcta cgcgcactac ggactcgggc
     6601 atctcgcggg catctatgac tccgggatct gggcgagcaa tgtcgagtgg ctcatcgcgc
     6661 cagtcgtcgt cggcgccccg ctcggattga tcggcgccct ggcccgccga cggagtccat
     6721 gggggctgct cgcccgactg atcgtcccgt taggcgcgct ggccgaaccc tgggtggtgt
     6781 cgatgtggcc ctggctcagc cagccgctga cgggctggcc gacacggatc gccgaaaccg
     6841 tgaccgcggt ggtcctgacg gctttcggtc tcgtgctggg cacgcgcgtg ctggcaagga
     6901 agccgcggac acggtgaacc cggcgctgcc gagccggctc atcgccgggc gacgacgcgg
     6961 gagctgaatg cctgcgccgt cacgaagccg agtcggtgat agatctcgcg ggcgcggtcg
     7021 ttgtcggagt acatcccgag cgcgcacatg ccgacctcgg agattgcgcg ccgggtcagg
     7081 gcggcggtca ctgcagcgcc ccagccgtgg ccacgctgct cggaggcgac ggtgatcccg
     7141 gagagttcgg gtatgccggc cgcggagggc tcgctgcacc cgcaggcgat cacgtggccg
     7201 gcggggtcgc gtgcgccgag ccagagctgg ccgtcgcggc ggaacggctg gccgtcggtg
     7261 cgggaattgt gttcggcgag aaaggtatcc aactcctcct tggccgtcgc gtcgaattcg
     7321 atcaggtcgt cttcggcggg ttgggccggc ggtggggtgg tggtggacat ccactcccaa
     7381 tccccgccgg gaccggcccg gaagatctcc tcgacatcgg cgcgcagtct cgctggcacg
     7441 ctgagcatcc cggtcgggcg ctcccgaaag aggagcgcgg ccagctgcgg gtcgcggagc
     7501 aatatccggg catccgcctc cgggccgagg agggtcagca cgatcccgcg tatgtggctc
     7561 ggccgggcga aggcgatcgc gttgccgagc acgacggcgt ccagcggctg atccgatgga
     7621 atctcggcgc gcaccatcgg gtgctcgccg ctggccgccc agagttcgcc gaagctggtc
     7681 accgggcgcg cgttcactcg cccaacgcta tcccggcacc cgtggggcct cgggcctcgg
     7741 cgtcgtcctc gtcgttaacc gtgagctcga cctgggcggc agcgctgctg ctgccggttc
     7801 atgccggttg tcggtgggtc tgcgtatgct gtcttcgggg ccgtcatagg tgttggctta
     7861 tcactcgaat tgcgttgcag cagaatgatt tgcttgtgga aaacgcttgt cggacaggta
     7921 gaactggagt acgatgaggc atggtcgaga tcaccgcatc cacctccgac cgggcagccg
     7981 cccgcgccgg tgggagtggc tgtcttgacg cggctggatg tcttgacgcg gctgggggcg
     8041 tcggtgtgac tgggggcgtc ggcgggcgac gcgagccggg gtgggtgccg ccgccggact
     8101 ggttcgacga gagcctgttc gccgagccgt ccgcctacga gtgcgagctg gccgaggagc
     8161 aggcgctgat cgagcacgtg agccggtgtg ccgatgacgg catacgggac tatgtcgccg
     8221 atccc
//
LOCUS       DAOPJG010000153         8077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9463_length_8077_cov_7.816754, whole genome shotgun sequence.
ACCESSION   DAOPJG010000153 DAOPJG010000000
VERSION     DAOPJG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9463_length_8077_cov_7.816754"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..500)
                     /locus_tag="PKU75_12640"
     CDS             complement(234..500)
                     /locus_tag="PKU75_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPF81772.1"
                     /translation="MSGPLPGEKEGGDERGPTPLERKDAGHPGQTTPPMWDALSYVLA
                     GPLLFGGVAYLLDRWVGTSWLVLPGVLIGMAVSLYTVWVRYGRQ"
     gene            complement(497..967)
                     /locus_tag="PKU75_12645"
     CDS             complement(497..967)
                     /locus_tag="PKU75_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81773.1"
                     /translation="MSVPAAVGPPNPFAAMTRTAFIPTLLVGPIAIAIAFFAGGGRAA
                     GGAALGYAVTIAFFLLGMIVMNRFAANTPPLLFMATALAVFFGQLIFLLLVILFLQNA
                     DWLDGRAFGLTALAVALVWQIFQVVGYLRTRRLVFDEPTTPASSPSEASPRHTA"
     gene            complement(992..2116)
                     /locus_tag="PKU75_12650"
     CDS             complement(992..2116)
                     /locus_tag="PKU75_12650"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPF81774.1"
                     /translation="MREYIFVLIVAMATTYLAVPAAYRLAVRFGAFTQVRDRDVHAVP
                     IPRLGGVAIFAGFAAATFTASKLPFLTQVFDTPELWGVLLGAGVICLLGAIDDLRELD
                     PLTKLGGQIVAAAIMAFFGVQLFSLPVFGVTTVLPAPVLATLTIFAVLVSTNAVNFID
                     GLDGLASGVVAIAAATFFAYSYYISRGFDPPNVFSSASFISAALLGCCLGFLPHNFYP
                     ARLFMGDSGALLLGLLLAAAMISTTGGGSPAQVSGNEVGAALLPLLVPFAIILLPLLD
                     VVLAIVRRTRAGRHPWSPDAQHLHHRMLQIGHGHRRAVLLLWLWAAVASFGCVAFVFL
                     PPLVAGSILIAALALTASLTRWLPRFSAAGHRPKTFAPDV"
     gene            complement(2139..3407)
                     /gene="glyA"
                     /locus_tag="PKU75_12655"
     CDS             complement(2139..3407)
                     /gene="glyA"
                     /locus_tag="PKU75_12655"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPF81775.1"
                     /translation="MTFYGSDYDALEAFDPEIAGVLMSELDRLRGGLQLIASENLTSP
                     EVLTAMGSVLSMKYAEGYPGRRYYGGCAEVDKAENLAIERCKALFGADHANVQPHSGA
                     SANQAVYGAFLKPGETLLAMSLPHGGHLTHGTKVSFSGKWFNAVHYGVDAETEDIDYD
                     QVEALAKEHRPTVILAGGSAIPRLIDFERFRAIADEVGAIFWVDAAHFIGLVAGKAIP
                     SPVPYADVVSFTTHKVLRGPRSGAIVCTEEHKAAIDKAVFPMMQGGPMMHVIAAKAVN
                     FKECGTPEYAAYANQVVDNASVLATTLGEHGIRPTTGGTDTHLSLHDLQGVGVSGKDA
                     EARADAAGIVLNKNAIPNDPAPPSVASGIRVGTPCVTTQGMGTEEMKTIAGLIAKAVT
                     EGDADPGHPVSQEVRAAVTDLVMRFPAYPR"
     gene            complement(3462..4202)
                     /locus_tag="PKU75_12660"
     CDS             complement(3462..4202)
                     /locus_tag="PKU75_12660"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPF81776.1"
                     /translation="MSPVVDASTPESQEIAVEAAVSAVRSGHLVVMPTDTVYGIGCDA
                     FDAGAVQALLDAKDRGRDVPPPVLIGALRTLDGIATDIPPYVRDLVERFWPGALTVVL
                     TAQPSLHWDLGETGGTVAVRMPDDPIALEILEQTGPMAVTSANKHGMPPATSVLDAAT
                     QLGSAVEVYVDGGPRTSQEPSTILDCTGAEPVVLRAGAISEDDLLAVTAPIEAEPEHV
                     NEVHDEAEVHDQTGGAGVDSAGSEPPQQ"
     gene            complement(4245..5375)
                     /locus_tag="PKU75_12665"
     CDS             complement(4245..5375)
                     /locus_tag="PKU75_12665"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HPF81777.1"
                     /translation="MSQAPAAPASTALAEDPAERPSRVGSLDAARGVMLILSVSAEAV
                     LAPRPEAFVHTKWVGLHYYDWIFPLFVTLSGCGMAFAFRNHVRWRRNARRALVLVLAG
                     LAYNYVAAPERGLADLRFTGVLQLYAALVVLVALLHHWLRSARAWIAFTLVWSGLLTL
                     FWFAVSRDCVGGMPQPTCNPTLTVEGAWLGGLDHLYRLGKRGFETEGILGILGALSIA
                     SAGVVVGHLLADRHRQRHTVARLLAWAATVAIAGWAFAQVVPALKWLWTPSYGLITAT
                     AGVLLLTLAYAVVDTGPHRAIGYRLAYPLRALGRNSLLVYFGSHVAMMIMLRLGEPSL
                     PERLSAKLGWTGHPHLAWVIASVLAWWALALVLDRKRIYVRA"
     gene            complement(5394..6242)
                     /locus_tag="PKU75_12670"
     CDS             complement(5394..6242)
                     /locus_tag="PKU75_12670"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPF81778.1"
                     /translation="MRTTHAPSATRNLPITRAGIPAAPAVFVSPHQDDELLFMGAAIR
                     EHVARGRDVRVLLIGRGNKSSVRTRPCLQEMLDRTPCEKEFGAVRDREFEWSVRGLGA
                     TPIIPTWEDRLDENAFTPTDVAAIIRKHAPSGSDLKTVSEYGDEYHSDHFACHDAVAS
                     LFAGGWTDMPPRYYTLCFRKQAVLDAGLRVVREGVSTPIAPEHQEPYKFVNVEESRWG
                     VGYTSVRKFFSAQARDPFSYRVVETEPARLPALTTSPREPVAAPRPAPSPNVPARARG
                     VLVPGA"
     gene            6554..7471
                     /locus_tag="PKU75_12675"
     CDS             6554..7471
                     /locus_tag="PKU75_12675"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPF81779.1"
                     /translation="MTTTEAIAVIANPSKPQWRKAIQAIERECAEPPAVITTTVEDPG
                     AGVARQAIADGAQRLVVCGGDGTVREVAAGTAGSGIPVGVVPIGTANIFARNIHLAPR
                     ALAANAARAVHGEARALDLGWATLHTADGGERRLPFLVLAGIGNDAATVLGTRAIIKS
                     RVGWLAYFESGARHLLHRPIPMEVALDDEPARQVQAWSVLAANCPRVPPGILVFPGAD
                     PTNGRLETLVASVRWPHQWAAVAAQGLRMPVRASALRYAACSRLRVTPSRPVPVQVDG
                     DAVAGVISLTVTIDPASVRIVAGSQRPSA"
     gene            7792..>8077
                     /locus_tag="PKU75_12680"
     CDS             7792..>8077
                     /locus_tag="PKU75_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81780.1"
                     /translation="MSVIELPRSTAREREHQQWWEDATVIAGELNHWHAQLVDLTARV
                     IAADAWGAHGGIRSPAHWLALRAGLSPATAAHIVTLAQARARMPIVTGLFE"
BASE COUNT         1284 a         2689 c         2977 g         1127 t
ORIGIN      
        1 cgacagggcc atcatcagca tgggccgggt gaaggtccaa tcaccactgc cgaagatcgg
       61 ccagtagaac tcttcgggtg acggggcgtg gaagccttcc gactcaccct cgctggcaag
      121 ggctaggcct accaggctca gactcacgtg gtctcctcgg acgatctgga catgcatgac
      181 cccgtgccag agcaccactc accgggggac gcgagcggag gtggtcgggg aaatcactgc
      241 ctaccgtacc ggacccatac cgtgtaaagc gaaacggcca taccgatcag gacacctggc
      301 aagacgagcc acgacgtgcc gacccagcgg tcgagcaagt aggccactcc gccgaacaag
      361 agcggccccg ccaggacata cgagagcgcg tcccacatcg gcggcgtcgt ctgcccgggg
      421 tgccccgcgt ccttgcgctc gagcggggtg ggcccccgct cgtccccgcc ctccttctcg
      481 cccggcagcg ggccgctcat gcggtatgcc gcgggctcgc ctcggacggc gaactcgccg
      541 gtgtcgtggg ttcgtcgaag acgaggcgcc gggtgcgcag gtagccgacg acctggaaga
      601 tctgccacac cagcgccacc gcgagggcgg tcaggccgaa cgcccggccg tcgagccagt
      661 cggcgttctg caagaacaag atcacgagca gcaggaagat cagctgcccg aagaagacgg
      721 ccagcgcggt ggccatgaac agcagcggcg gggtgttggc ggcgaaccgg ttcatgacga
      781 tcatgccgag caggaagaag gcgatcgtca cggcataccc taaagccgcc ccacccgcgg
      841 cgcgcccgcc ccccgcgaag aacgcgatgg cgatcgcgat cggcccgacc agcagggtcg
      901 ggatgaacgc ggtgcgggtc atcgcggcga aggggttcgg cgggccgacc gctgcaggaa
      961 cgctcaccgc gccattatcg ctgcttgcgc gtcatacgtc gggcgcgaac gtcttgggcc
     1021 ggtggcccgc cgcggagaaa cggggcagcc agcgggtcag gctcgccgtg agcgcgaggg
     1081 ccgcgatcaa gatgctgccg gccaccagcg gagggaggaa gacgaaggcg acacagccga
     1141 aggacgcgac ggcggcccag agccacagca gcaggaccgc acgccggtgt ccgtggccga
     1201 tctgcagcat gcggtgatgc aggtgctggg catcggggct ccacgggtgg cggccggcgc
     1261 gggtgcggcg cacgatcgcg aggacgacgt cgagcagcgg caacaggatg atcgcgaacg
     1321 gcaccagcag gggcagcagc gccgcaccga cctcgttgcc ggagacctgg gccgggctgc
     1381 ccccgcccgt cgtggagatc atcgccgccg ccagcagcag cccgagcagg agggcgccgc
     1441 tgtcgcccat gaagagccgg gccggataga agttgtgcgg caggaagccg aggcagcacc
     1501 cgagcagggc ggcagagatg aagctcgccg agctgaacac attgggcggg tcgaacccgc
     1561 gggagatgta gtagctgtag gcgaagaagg tggcggccgc gatcgcgacg acgccggagg
     1621 cgagtccgtc gaggccgtcg atgaagttga cggcgttcgt cgagacgagc acggcgaaga
     1681 tcgtcagggt cgcgagcacc ggcgccggga gcaccgtcgt gacgccgaag accggcaggc
     1741 tgaagagttg aacgccgaag aaggccatga tggcggcggc gacgatctgc ccgccgagtt
     1801 tggtgagcgg gtccagctcg cggaggtcgt cgatggcgcc gagcaggcag atcacacccg
     1861 ccccgagcag cactccccac agctcagggg tgtcgaaaac ctgtgtcagg aaaggcagtt
     1921 tcgacgcggt gaaggttgcc gcggcgaagc cggcgaagat cgccactccc ccgagccgcg
     1981 ggatcggcac ggcgtgcacg tcgcggtcgc gcacctgggt gaaggccccg aatcgcaccg
     2041 ccagccggta tgccgcgggc acggcgaggt aggtcgtcgc catcgcgacg atcaggacga
     2101 agatgtactc gcgcacccgc ggtcaggccg gccgagcgtc agcgcgggta tgccgggaag
     2161 cgcatcacga ggtcggtcac cgcagcccgg acttcctggg agaccgggtg gccggggtcg
     2221 gcgtccccct cggtgacggc cttggcgatc aggcccgcga tggtcttcat ctcctcggtc
     2281 cccatgccct gggtcgtgac gcagggcgtg ccgacgcgga tgccggaggc gaccgacggc
     2341 ggggccgggt cgttggggat ggcgttcttg ttcaagacga tgccggccgc gtcggcgcgg
     2401 gcctcggcgt ccttgccgct gaccccgacg ccctgcaggt cgtgcagcga caggtgggtg
     2461 tcggtgccgc cggtggtggg ccggatgccg tgctcgccca gcgtggtcgc caggaccgag
     2521 gcgttgtcca cgacctggtt ggcgtacgcg gcatactccg gcgtcccgca ctccttgaag
     2581 ttgaccgcct tggccgcgat gacgtgcatc atcggcccgc cctgcatcat cgggaagacc
     2641 gccttgtcga ttgcggcctt gtgctcctcg gtgcagacga tggcgccgga gcgcgggccg
     2701 cgcagcacct tgtgcgtggt gaaggagacg acgtcggcgt agggcacggg actcgggatc
     2761 gccttgccgg cgacgagccc gatgaagtgc gcggcgtcga cccagaagat cgccccgacc
     2821 tcgtcggcga tggcgcggaa gcgttcgaag tcgatcagcc gcgggatcgc cgagccgccg
     2881 gcgaggatca ccgtggggcg gtgctccttg gcgagggcct cgacctggtc gtagtcgatg
     2941 tcctcggtct cggcgtcgac gccgtagtgg acggcgttga accacttgcc ggagaaggag
     3001 accttggtgc cgtgggtgag atgcccgccg tgcggcagcg acatcgcgag gagggtctcg
     3061 cccggcttga ggaaggcacc gtagacggcc tggttggcgc tggcgccgct gtgcggctgg
     3121 acgttggcgt ggtcggcacc gaagagggcc ttgcaccgct cgatggcgag gttctcggcc
     3181 ttgtccacct ccgcgcagcc gccgtagtag cggcggccgg ggtagccctc ggcgtacttc
     3241 atcgacagca ccgaccccat ggcggtgagg acctcgggcg aggtgaggtt ctcgctcgcg
     3301 atcagctgca gaccgccgcg gagccggtcc agctcgctca tcaggacgcc ggcgatctcc
     3361 gggtcgaagg cttcgagggc gtcgtagtcg gaaccgtaga aggtcatcgg gcttcccttg
     3421 tcgtcgggcc ggtcggcggg tgtgatccgg ttccgcccac tctattgctg cggcggctcg
     3481 gagccggcgc tgtccacgcc cgccccgcca gtctgatcgt gcacctcggc ctcgtcgtgc
     3541 acctcgttca cgtgctccgg ctcggcctcg atcggcgccg tcacggccaa caggtcgtcc
     3601 tcgctgatcg ccccggcgcg caacacgacc ggctcggcgc cggtgcagtc gaggatcgtc
     3661 gacggctcct ggctggtgcg cggcccgccg tcgacataca cctccacggc cgagccgagc
     3721 tgggtggcgg cgtccaggac gctggtggcg ggcggcatac cgtgcttgtt cgcgctggtg
     3781 acggccatcg gaccggtctg ctcgaggatc tccagggcga tcgggtcgtc gggcatccgc
     3841 acggccaccg tcccgcccgt ctcccccagg tcccagtgca gggacggctg agccgtcaac
     3901 acgaccgtga gcgcgccggg ccagaaccgc tccacgaggt cacggacata cggcggaatg
     3961 tcggtggcga tcccatccag ggtgcgcaac gcgccgatga gcaccggcgg cggcacgtcc
     4021 cgcccgcggt ccttggcatc gagcagcgcc tgcacggccc ccgcgtcgaa ggcgtcgcac
     4081 ccgatgccgt agacggtgtc ggtcggcatc acgacgaggt gcccgctgcg caccgcgctg
     4141 accgccgcct cgacggcgat ctcctgactc tcgggcgtgc tggcatcgac caccggactc
     4201 atgggcgtga gtctaaagtc tgtacgcgcc gccaccatgc gggttcaggc ccgcacgtag
     4261 atccgcttgc ggtcgagcac cagcgcgagc gcccaccagg ccaggacgga ggcgatcacc
     4321 caggcaaggt gggggtgacc ggtccagccg agtttcgccg agagtcgctc ggggaggctg
     4381 ggctcgccca gtcgcagcat gatcatcatc gccacgtgcg agccgaagta gaccagcagg
     4441 ctgttgcggc ccagggcccg gagcggatag gccagccggt agccgatcgc gcgatggggt
     4501 ccggtgtcga cgacggcgta ggccagggtg agcaggagca cgccggcggt cgccgtgatc
     4561 aggccgtatg acggggtcca caaccacttc agggcgggca cgacctgggc gaaggcccag
     4621 ccggcgatgg cgacggtggc cgcccacgcg agcaaccggg cgacggtatg ccgctgccgg
     4681 tgccggtcgg cgagcaggtg gccgacgacc acgccggccg aggcgatgga cagtgcgccg
     4741 agtatgccga ggatcccctc ggtctcgaag cctcgcttcc cgagccggta gaggtggtcg
     4801 aggccgccca gccacgctcc ctcgacggtg agggtggggt tgcaggtggg ctgcggcata
     4861 ccgccgacgc agtcgcgcga gacggcgaac cagaacaggg tgagcagacc cgaccacacc
     4921 agcgtgaagg cgatccacgc ccgtgccgag cggagccaat ggtggagcag cgcgacgagg
     4981 acgaccagcg cggcatacag ctgcagcact ccggtgaagc gcaggtcggc gagcccacgt
     5041 tcgggcgcgg cgacatagtt gtatgccagc ccggcgagga cgagcacgag ggcgcggcgg
     5101 gcgttgcggc gccagcgcac gtggttgcgg aaggcgaacg ccatgccgca gccggagagg
     5161 gtgacgaaga gcgggaagat ccagtcgtag tagtgcaggc cgacccattt ggtgtggacg
     5221 aaggcctcgg ggcggggcgc gaggacggcc tcggcgctga cgctgaggat gagcatgacc
     5281 ccgcgggcgg cgtcgaggga cccgacacgc gaggggcgct cggccgggtc ctcggccagg
     5341 gcggtcgagg cgggggccgc gggtgcctgg ctcacggcac ccagggtcac atgtcaggca
     5401 cccggaacca agactccacg cgcgcgcgcc gggacgttgg gcgatggtgc gggccgcggc
     5461 gccgccacgg gctcgcgggg cgacgtggtg agcgccggga ggcgagcggg ctcggtctcg
     5521 accacgcgat aggaaaaggg gtcgcgggcc tgcgcgctga agaacttgcg cacggatgtg
     5581 tagccgacac cccagcgcga ttcctcgacg ttgacgaatt tgtagggttc ctggtgttcc
     5641 ggggcaatcg gtgtcgaaac accctcgcgg acgacgcgta gcccggcgtc aaggacggcc
     5701 tgcttgcgga agcacagtgt gtaatagcgc ggcggcatgt cggtccaacc gccggcgaag
     5761 agcgaggcga cggcgtcatg gcacgcgaaa tggtcgctgt ggtattcgtc gccgtattcg
     5821 ctgacggtct tcagatcgct gcccgacggc gcgtgtttgc gaatgatcgc ggccacgtcg
     5881 gtgggggtga aggcgttctc gtcgagccgg tcctcccagg tggggatgat gggcgtggcg
     5941 ccgagaccgc gcaccgacca ctcgaactcc cggtcgcgaa ccgcgccgaa ttccttctca
     6001 cacggcgttc ggtcgagcat ttcctgcagg cacggccgag tccggacgga ggatttgttt
     6061 ccgcggccga tgagcagcac ccgcacatca cgaccccgag ccacgtgttc ccggatcgcc
     6121 gcgcccatga acagcagttc gtcgtcttga tgaggcgaga cgaaaacggc cggggcggcg
     6181 ggaatcccgg cccgggtgat cgggagattt cgggttgccg acggcgcgtg cgtcgtcctc
     6241 atcgctcctc ctcgtgcatt cccgtccgac gtgggatgag aaattagagg aggaccggcg
     6301 cggctcggcg ccgctgcgcg gagtgtcgcg tcatcgcaac agtactgtta cacatgcgac
     6361 tggtgggatg gcagtgattc cagaggcccc cggcttcggg ctcggaggct caccgatatc
     6421 ggacgcacac ccggtggtga cggcgcacga ccggtggtga cacggtcttg tgttgcgtca
     6481 cgtcccagcg gtgcgaggtg gtgccgcgcc cggtgactag ggtggcccgt atgcggatgg
     6541 gccgggccac gcggtgacga cgaccgaagc gatcgccgtc atcgcgaatc cgtcgaaacc
     6601 gcagtggcgc aaggcgatcc aggcgatcga gcgggagtgc gcggagccgc cggccgtgat
     6661 caccaccacc gtcgaggacc ccggcgccgg ggtggcacgg caggcgatcg cggacggcgc
     6721 gcagcgattg gtcgtctgcg gtggcgacgg cacggttcgc gaggtcgccg cggggacggc
     6781 gggcagcggc ataccggtgg gtgtcgtccc gatcgggacc gcgaacatct tcgcccgcaa
     6841 tatccacctc gccccccgcg ccctggcggc gaacgccgcg cgggcggtcc acggggaggc
     6901 gcgcgctctc gacctcggct gggccacgct gcataccgcc gacgggggcg agcggcgcct
     6961 gcccttcctg gtcctcgccg gcatcggcaa cgacgccgcc accgtgctcg ggacgcgcgc
     7021 gatcatcaag tcccgcgtcg gctggctcgc ctacttcgag tccggcgccc gccacctgct
     7081 gcaccgcccc attccgatgg aggtggcgct cgacgacgaa ccggcacggc aggttcaggc
     7141 gtggagcgtg ctcgccgcga actgcccccg cgtcccaccg ggcatcctgg tcttccccgg
     7201 cgccgacccc acgaacggcc gcctggagac gctcgtcgcg agcgtccgct ggccgcacca
     7261 gtgggccgcc gtcgccgcgc aaggcttgcg gatgccggtg cgcgccagcg ctttacggta
     7321 tgccgcgtgc tcccgcctgc gtgtcacgcc cagtcgcccc gtgcccgtgc aggtcgacgg
     7381 cgacgccgtc gcgggcgtca tctcacttac ggtgaccatc gacccggcga gtgtgcggat
     7441 cgtggccggc tcacagcgtc cttctgcctg accttcgcgg gtgggagccc gggcggccgc
     7501 gggccggccg cgtgggccgg tctcgggggc cggccggacg tctgtcggag aggttgtcgg
     7561 accctctcgc tagggtggcg gtaacgcaac ccggggcacc cttcccgtga cgcattcacc
     7621 tgcgaaaaag gttgcgccgc aacacaaccg ggttgcacca ggactagaac tggtgtacga
     7681 cgaacagatc tctcgacacc ggcgtcggga ggcccagacg gctggcacca cgggcggggt
     7741 gcggtcgcca ccggaaactg ggtacgtcga gatgactggg ggtctttcga gatgagcgtt
     7801 atcgagctac cgcgctccac cgcgcgcgaa cgtgaacacc agcagtggtg ggaggacgcc
     7861 acggtcatcg cgggcgagct gaaccactgg catgcgcaac tggtcgacct gaccgcgcgg
     7921 gtgatcgccg ccgacgcgtg gggcgcccac ggcgggatcc gttccccggc acattggctg
     7981 gcgctgcggg ccgggctctc cccggccacc gcagcccaca tcgtgaccct tgcccaagcc
     8041 cgcgcgcgga tgcccatcgt caccggcctg ttcgagt
//
LOCUS       DAOPJG010000154         8071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9469_length_8071_cov_7.771831, whole genome shotgun sequence.
ACCESSION   DAOPJG010000154 DAOPJG010000000
VERSION     DAOPJG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9469_length_8071_cov_7.771831"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..745)
                     /locus_tag="PKU75_12685"
     CDS             complement(104..745)
                     /locus_tag="PKU75_12685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF81781.1"
                     /translation="MPPRRLILLRHGQTQSNLDGIWQGHLDVELTELGRRQADAAAAQ
                     LTAYRPTTIVSSDLKRAAVTAQAVARDLSLTVAFDPRWREFHVGQWTGKTTAEVIAMH
                     PAERLAALRGEEQRRGIDGEGPAEVRARVTPALDELIGRLGEGECAIIVSHGGTIRVL
                     AAVLLGLDMLTATRLLTTPGNCQWADLVQAPAGWRLAGWNLRAPDLGAGEVSG"
     gene            complement(757..1164)
                     /gene="rsfS"
                     /locus_tag="PKU75_12690"
     CDS             complement(757..1164)
                     /gene="rsfS"
                     /locus_tag="PKU75_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPF81782.1"
                     /translation="MPATDQAKDLATVAAHAAADKLASTIVALDVSGQLPLTDIFVIV
                     SAPTERQVAAIVDEVEEKVRLAGTKVKRTEGAREGRWVLLDFGDIVVHIQHEDERQFY
                     DLERLWRDCPQVDVGVVGADQAAATAARSAVQD"
     gene            complement(1151..1762)
                     /gene="nadD"
                     /locus_tag="PKU75_12695"
     CDS             complement(1151..1762)
                     /gene="nadD"
                     /locus_tag="PKU75_12695"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPF81783.1"
                     /translation="MGGTFDPIHHGHLVAASEVQATFGLDEVVFVPTGEPWQKEGRNV
                     SPAEHRYLMTVVATASNPRFTVSRVDIDRPGKTYTIDTLKDLHEQRPDTELYFITGAD
                     ALAQILSWKDVDALFGLAQFVGVTRPGYELSEAGLPGDRVHLQEVPAMAISSTDCRER
                     VARHEPVWYLVPDGVVQYINKHQLYAAAPASGPSAPKETRARD"
     gene            complement(1825..2046)
                     /locus_tag="PKU75_12700"
     CDS             complement(1825..2046)
                     /locus_tag="PKU75_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81784.1"
                     /translation="MSTSVLMLASEGAETMHELPMPAYMYAVIAIALFALGLAVVWFF
                     RGTAAKYGSAHLPDDADGHEHGTTRGSHH"
     gene            2605..3552
                     /locus_tag="PKU75_12705"
                     /pseudo
     CDS             2605..3552
                     /locus_tag="PKU75_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            3549..4352
                     /locus_tag="PKU75_12710"
     CDS             3549..4352
                     /locus_tag="PKU75_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPF81785.1"
                     /translation="MSVRFERDERGGVTVWVHGQPQSYVAPHDPGLLAFEYVEQLGAV
                     VDSIAPEGDRIAVTHVGGAGLTLPRYVEFTRPGSTQIVFEPDAVLTEHVRRELPLPRG
                     HRIRVRPLDGRAGLSAVSDASADLVVVDAFSDGAVPAELTTVEFYREALRVLRPDGLL
                     AANVTDALDRRYLVRVLAGVLEVASACVVLATHDVLKGRRFGNYVIVAGQGPLPLREL
                     SRRVAKTAAPAGMWLPARVSALSRSARPSTDADPARCPPAPDPGSWRVR"
     gene            complement(4413..5105)
                     /locus_tag="PKU75_12715"
     CDS             complement(4413..5105)
                     /locus_tag="PKU75_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF81786.1"
                     /translation="MHDKEVRKLTELEDTHWWYAERRHRLARSIAGMTPGRALDIGAA
                     GGGNSRVLAREGWDVLALEYGAEGAQVGKERGLDVIRADATQLPLDRECVDLVVAYDV
                     LEHIPDHDAAVREVHRILRPDGTFLIAVPCDPRLWSAHDVAVDHVRRYTRGTLSELLL
                     RNRFRIEELWSWNVLLRPTVALRRRTSTGSDLEQLPRPVNLALKGVVTVERYLPVKGL
                     PGVTLMVTARPA"
     gene            complement(5105..6388)
                     /locus_tag="PKU75_12720"
     CDS             complement(5105..6388)
                     /locus_tag="PKU75_12720"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPF81787.1"
                     /translation="MSEISPEATATVAELAQKSRVAARELALLSRARKDEVLRGLADA
                     LDSATARVVAANEEDLERGRAGGMPESLQDRLRLTPERVAAVADALRDIAALPDPVGE
                     IVRGSTLANGLQIRQVRVPMGVVGMIYEARPNVTVDAAGLGLKSGNAVILRGGSAAAS
                     SNRVLVDVMRESLSAQGINPDAIGLLDGGGHDAVRALMTARGLVDLLIPRGGADLIQS
                     VVLNSTVPVIETGIGNCHVYVDRAADLDKALAITVNSKTQRPSVCNAAESLLVHAEVA
                     DEFLPKALTDLAGASVTLHTDERASAIATAAGVPFAVATQQDFATEYHSLEMSVGVVD
                     SIDAALEHVRTFGSGHTEAIVTEDRAAARRWTTEVDAAAVMVNASTRFTDGGEFGFGA
                     EIGISTQKLHARGPMALPELTTTKWIVEGDGHVRI"
     gene            6544..7056
                     /locus_tag="PKU75_12725"
     CDS             6544..7056
                     /locus_tag="PKU75_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81788.1"
                     /translation="MSRRFATALAGVALSAPLAITAPADAAALRTCRGTIANIVVSDS
                     IRVPAGASCTLRNVAARGAVFVESGATLIVTNSQVARSVTSTGHRKVSIENTRVTGAV
                     SLNKGGAISILYSSFGADITLTANTKTGTKVLRGNGIDGRLSCTLNAPAPTGSLNSVR
                     GGKFGQCKRV"
     gene            7088..7942
                     /gene="pdxY"
                     /locus_tag="PKU75_12730"
     CDS             7088..7942
                     /gene="pdxY"
                     /locus_tag="PKU75_12730"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HPF81789.1"
                     /translation="MTTIVSIQSAVAYGHVGNSAAVFPMQRLGVEVWPVYTVNFSNHT
                     GYGAWKGPLIAADDVRAVITGMEERDAFGEVDAVLSGYQGGNEIGGVIIDAVARVKAA
                     NPSAIYACDPVMGNAKSGCFVHPDIPILLREKVVPVADLITPNQFELGFLTHTEPETL
                     EDTLASVDKAREMGPSTVLVTSVLRPDRPEDTIEMLAVHGDEAWLVQTPHIPFKVNGS
                     GDVTAALFTAHLLDSGRADDALARTVSSVYAVLKRTFESGRRELQLVQSQEEIAHPAM
                     EFTPERVR"
BASE COUNT         1166 a         2825 c         2813 g         1267 t
ORIGIN      
        1 ccctgacccc ctccatgcca tggaggtgcg ctaccaactg cgctacagcc ccgtaaccgg
       61 gtgccggccc aggagattca ctcgcgagcg cgaccgacag atcttaccca gagacctcgc
      121 cggcgcccaa atcgggggcg cgcaggttcc acccggccag ccgccagccc gcgggcgcct
      181 gcaccaggtc ggcccactgg cagtttcccg gggtggtcag caggcgcgtc gcggtgagca
      241 tgtcgagacc gaggagcacg gcggcgagga cgcggatggt gccgccgtgg gagacgatga
      301 tcgcgcactc cccttctccc aagcggccga tgagttcgtc gagggccggc gtgacgcggg
      361 cccggacctc ggcggggccc tcaccgtcga tgccgcggcg ttgctcctcg ccgcgcagcg
      421 ccgccagccg ctcggcgggg tgcatcgcga tgacctcggc cgtcgtcttg cccgtccatt
      481 ggcccacgtg gaactcgcgc cagcgggggt cgaatgcgac cgtgagcgac aggtcgcgcg
      541 ccaccgcctg ggcggtcacg gcggcccgtt tcagatccga ggagacgatg gtcgtcgggc
      601 ggtatgccgt cagctgcgcc gcggccgcgt cggcctggcg ccgcccgagc tcggtcagct
      661 ccacgtcgag gtgaccctgc cagatcccgt cgaggttgga ctgcgtctgc ccgtgacgaa
      721 gcaggatgag gcgacgcggc ggcataccgg ggaagctcag tcctggacgg ccgaacgggc
      781 ggccgtcgcc gcggcctggt cggcgccgac gacgcccacg tcgacctgcg gacagtcacg
      841 ccagagccgc tcgaggtcgt agaactgccg ctcgtcctcg tgctggatgt gcacgacgat
      901 gtcgccgaag tcgagcagca cccaccggcc ctcgcgggct ccctcggtcc gcttcacctt
      961 cgtgccggcc agccggacct tctcctcgac ctcgtcgacg atggcggcga cctggcgctc
     1021 cgtcggcgcc gagacgatga cgaagatgtc ggtcagcggc agttgcccgc tgacgtcgag
     1081 agcgacgatg gtcgaggcga gtttgtcggc ggccgcgtgg gcggcgaccg tggcgaggtc
     1141 cttggcctgg tcagtcgcgg gcacgggtct ccttcggggc ggatgggccg ctggcgggtg
     1201 cagcggcata cagctggtgc ttgttgatgt actggacgac gccatcgggc acgaggtacc
     1261 agaccggttc gtggcgggcg acacgctccc ggcagtcggt ggacgagatg gccatggccg
     1321 gcacctcttg caggtgcacc cggtccccgg gcagcccggc ctcggacagc tcgtagccgg
     1381 ggcgggtgac accgacgaac tgtgccaggc cgaagagcgc gtcgacgtcc ttccacgaca
     1441 ggatctgggc gagggcgtcg gcgccggtga tgaagtagag ctcggtgtcc gggcgctgtt
     1501 cgtgcaggtc tttgagggtg tcgatcgtgt aggtcttgcc ggggcggtcg atgtcgacgc
     1561 ggctgacggt gaaccgcgga ttggacgcgg tggccacgac ggtcatcagg tagcgatgct
     1621 cggcggggct gacattgcgc ccctccttct gccacggctc cccggtgggc acgaacacca
     1681 cttcgtcgag accgaaggtg gcctgcacct cgctggctgc gaccaggtgc ccgtggtgga
     1741 tcgggtcgaa ggtgccgccc atcaccccga tgcgtcggcc cgactgcgcc cgggcgcccg
     1801 cggtggatgg gtcgaccggg gcaatcagtg gtggctcccc cgggtggtgc cgtgctcgtg
     1861 gccatcggcg tcatccggca ggtgggccga gccgtacttc gccgcggtcc cccggaagaa
     1921 ccagacgacc gccagaccca aggcgaacag cgcgattgcg atgacggcat acatgtaggc
     1981 cggcatgggc aactcgtgca tcgtctcggc accctcggaa gccagcatca gcacactcgt
     2041 cgacatgcgc caaaccatac caacagcgca gcagccggca tctcccacgg cggttcatcc
     2101 acggatttac ccacagccct gtgcacgatg aggacggcac acgcggcggc tgtggacagc
     2161 cgcgcgtggc gggaagaaca cccccgcgga cggcattgcg ctggagcggc cggagggctt
     2221 aaatggaccc tcgtcacccg acgtcgagca cctgcgaaac gtcaggtgga ggttgcggag
     2281 aaaacgaggc gcggccggtt cgttcacgga cgaccggccc tggatttttc gagatcgaca
     2341 gcctgtgtgc gacggcacgg gggcgccacg agacttcgcc gcctgctgtg tcagtgcatc
     2401 gtcgccgccc tccagggccc tcggtcggcg atggccagcg tcgcctcggc ggtgcggccc
     2461 ccttcccggg ccgcgccctg gcagacccgg cccctcgatc ctcatacatc gaggggccgg
     2521 aagcattttc accccacgca gcgaccgtgc ccacgcatgc cgtcgtgttc ttcggcgttc
     2581 ccggatgctt ctacgatggc ggccatggcc gccccccacc tgagcatcgt ggtgccgatg
     2641 ttcaacgagg aggaggtgct cccgctgttc atcgcccgcc tgcggcccgt cgcggacggc
     2701 ctgggggtgt cgtatgaagt ggtggcggtc gacgacggct cgcacgacca gacggcgatc
     2761 gggttgcagc gggcccggcg cgactggccg cagctgcggg tcatccggct gcgggccaac
     2821 agtggccacc aggccgccat ctcggccggg ctggcccggg cccgcggcgc ctatgtcgtc
     2881 accctcgacg ccgacctgca ggacccgccc gaagtcgtcg cggagatgct ggccgccgcg
     2941 caggagaagc atgtcgacgt cgtctacggc atccgcggtg accgctcgag cgactcggtg
     3001 ttcaaacgcg ccacggcccg gttcttctat cgctcgatgg acaagctcgc cgagaccggc
     3061 gcgcaccacg acgccggcga cttccgcctg atgtcgcgcg cgaccgtgga tgcgatcaac
     3121 gcccttccgg agtatggccg ggtgctgcga ttcgtcgtgc ccgcgctcgg cttcccgtcg
     3181 gcgtcggtga cctaccgccg cgacgagcgc gccgccggca gctcgaagta cccgttcgcc
     3241 aagatgctgc gcctctcgat cgactcggtc accgccttct ccctcgcccc cttgcgtttc
     3301 gccaccttcc tcgggctcgc cggaggtctc ggcgccctcg tcctcctgct ctacgccctg
     3361 tatgcctgag ccgccggcca tgtcgtggcc gggtggacct cgacggtggt catcgtcacc
     3421 gccgtcggtg cggtccagtt gctggctctc gggatcctgg gcgaatacgt cggtcgcctg
     3481 tatgccgcct cgcaggcccg gccgtcgtat tacgtcgcct acgactccga ggaggagcag
     3541 cgagcgccgt gagcgtgcgc ttcgaacgcg acgagcgcgg cggtgtcacc gtgtgggtgc
     3601 acgggcaacc gcagtcgtat gtcgcccctc acgaccccgg gctgctggcc ttcgagtatg
     3661 tcgagcagtt gggagccgtc gtcgattcca ttgcgccgga gggagaccgg atcgccgtga
     3721 cgcacgtggg cggggccggc ctgaccctcc cccggtatgt cgagttcacc cgccccgggt
     3781 cgacccagat cgtcttcgag ccggatgcgg tcctgacgga gcacgtacgc cgcgagttgc
     3841 cgttgccgcg tggtcaccgc atccgggtgc gccccctcga tggtcgcgcc ggcctgtccg
     3901 cggtgtccga tgcgtcagcg gatctcgttg tcgtagatgc cttttcggac ggcgccgtgc
     3961 ctgccgagct gacgacggtc gagttctacc gggaggcact ccgcgtgttg cgtccggacg
     4021 gactgctggc cgcgaatgtc acggacgccc tggatcggcg ctatctggtg cgggtgctcg
     4081 ccggtgtgct ggaggtggcc tcggcgtgtg tcgtcctggc gacccacgac gtcctcaagg
     4141 gccggcgctt cggcaactac gtgatcgtcg ccggccaggg cccgctgccg ctgcgcgagc
     4201 tgagccggcg ggtggcgaaa accgctgcgc cggcgggcat gtggctgccc gcgagggtga
     4261 gcgccctgag ccgctcagcg cgcccgtcga ccgacgccga tccggcgcgc tgcccgcccg
     4321 cgcccgaccc gggctcgtgg cgcgtccgct gacctccccc gttcgtccag tccgccgccg
     4381 ccgccgtcga cgggcgcggc ggcggcgcgt gatcaggccg gccgcgcggt caccatcaag
     4441 gtcacgccgg gcaggccttt caccggcagg tagcgctcca ccgtgaccac gcccttcagt
     4501 gccaggttca ccggtctggg cagttgctcg agatcgctgc cggtgctcgt gcgccggcgc
     4561 agggcgacag tcgggcgcag gaggacattc caggaccaca gctcctcgat cctgaatcgg
     4621 ttgcgcagca gcagttccga gagggtgccc ctggtgtagc gccgcacgtg atcgacagcg
     4681 acgtcgtgag cggaccacag ccgcgggtcg cacggcacgg cgatgaggaa ggtgccgtca
     4741 gggcgcagga tgcggtgcac ctcgcggacc gccgcgtcgt ggtccgggat gtgttcgagc
     4801 acgtcgtagg cgacgaccag atcgacgcat tcgcgatcca ggggcagttg cgtggcgtcg
     4861 gcccggatga cgtcgagtcc tcgctccttg ccgacctggg cgccctcggc gccgtattcg
     4921 agagcgagca catcccagcc ctcccgggcg agcacccggg agttgccgcc accggccgcg
     4981 ccgatgtcga gggcgcgacc gggcgtcata ccggcgatcg agcgagccag gcggtggcgg
     5041 cgctcggcat accaccagtg cgtgtcctcg agttcggtga gcttgcgaac ttccttgtcg
     5101 tgcatcagat tcgcacgtgc ccgtcgcctt cgacgatcca cttcgtcgtc gtcagctccg
     5161 gcaacgccat cgggccgcgg gcgtgcagct tctgcgtcga gatgccgatc tcggcaccga
     5221 acccgaactc cccgccgtcg gtgaagcggg tcgaggcgtt gaccatcacg gcggccgcgt
     5281 cgacctccgt cgtccagcgg cgggccgccg cgcggtcctc ggtcacgatc gcctcggtgt
     5341 gccccgaacc gaaggtgcgc acgtgctcca aggccgcatc gatgctgtcg acgaccccga
     5401 cgctcatctc gagcgagtga tactcggtgg cgaaatcctg ttgtgtcgca acggcaaagg
     5461 ggacgcccgc cgcagtggcg atggcgctgg cgcgctcgtc ggtatgcagc gtgacactgg
     5521 ctccggccag gtcggtcagg gccttgggca ggaattcgtc ggcgacctcg gcgtggacga
     5581 gcagggactc ggccgcattg cagacgctcg gccgctgggt cttgctgttg acggtgatgg
     5641 cgagcgcctt gtcgaggtct gccgccctgt cgacatagac gtggcagttg ccgatgccgg
     5701 tctcgatgac ggggacggtg gagttcagga cgacggactg gatgaggtcg gctcccccgc
     5761 gaggaatcag caggtcgacc aggccacgtg ccgtcatcag ggcccggacc gcgtcatgac
     5821 ctccgccgtc gagcagtccg atcgcgtcgg ggttgatccc ttgtgccgaa agggattcgc
     5881 gcatcacgtc cacgaggacc cggttggacg aggcggccgc cgacccaccg cgaagaatga
     5941 cggcattgcc ggatttcagg ccgaggccgg cggcgtcgac cgtgacattg ggccgggcct
     6001 cgtagatcat cccgaccacg cccatcggca cccgcacctg gcggatctgc aggccgttcg
     6061 ccagagtgga gccgcggacg atctccccga ccgggtcggg cagggcagcg atatcgcgca
     6121 gcgcgtcggc gaccgccgcg acccgctcgg gggtcaggcg cagccggtcc tgcaggctct
     6181 cgggcatccc gcccgcgcgg ccgcgttcga ggtcctcctc gttggccgcg accacgcgcg
     6241 cggtggcgga gtcgagggcg tcggcgaggc cacgcaacac ctcgtccttg cgggcacgtg
     6301 agagcagggc aagctcgcgc gccgcgacgc gcgacttctg cgcaagctcg gcgacggtgg
     6361 ccgtcgcctc gggcgagatt tccgacatgg ctccagggta ggccgttacc gcccggccac
     6421 ctggtgttcg cccacgaggc ggccagatgc ggcatgctct gacgcaaccg ggcgagggga
     6481 tcgcccggtg ccacccaacc tgtacgcatc ccacgtacat cacgttcgga cgaagaggac
     6541 gccatgtcac gacgctttgc caccgctctc gccggtgtcg cgctgagcgc gccgctcgcg
     6601 atcaccgccc cggcggacgc cgccgcactg cgcacctgtc gcggcacgat cgccaatatc
     6661 gtcgtgagcg acagcattcg cgtgcccgcc ggggcgagtt gcaccctgcg caatgtcgcc
     6721 gcacgcgggg ccgtcttcgt cgaatcgggc gcgacgctga tcgtcacgaa cagccaggtc
     6781 gcgcggagcg tgacgagcac cggacatcgc aaggtcagca tcgagaacac tcgcgtcacc
     6841 ggcgccgtca gcctcaacaa gggcggcgcg atctcgatcc tctacagcag cttcggggcg
     6901 gatatcacgt tgaccgcaaa caccaagacc ggcacgaagg tgctgcgggg caatgggatc
     6961 gacgggcgtc tttcgtgcac gctcaacgcg cccgcgccga cgggttcgct caattcggtc
     7021 cggggcggca agttcgggca gtgcaagcgc gtctgactcc gcccgagccg gtggcagaat
     7081 cctgcgtgtg accaccatcg tctcgatcca gtccgcggtc gcctacggcc acgtgggcaa
     7141 ctccgccgcc gtcttcccca tgcagcgcct cggggtggag gtgtggccgg tctatacggt
     7201 caacttctcc aaccacaccg gctacggcgc gtggaagggg ccgctgatcg cggccgacga
     7261 cgtgcgtgcg gtcatcaccg gcatggagga gcgcgacgcc ttcggcgagg tcgacgcggt
     7321 gctgtccggc tatcaaggcg gcaacgagat cggcggggtc atcatcgatg ccgttgcccg
     7381 cgtcaaggcc gccaatccgt cggcgatcta tgcgtgcgac ccggtgatgg gcaacgccaa
     7441 gagcggctgc ttcgtccacc cggacatccc gatcctgttg cgcgagaagg tggtccccgt
     7501 cgccgacctg atcactccga atcagttcga actaggcttc ctcacccaca ccgagcccga
     7561 gacgctcgag gacacgctcg cctccgtcga caaggcccgc gagatgggcc cctcgaccgt
     7621 gctcgtcacc agcgtgctgc ggccggaccg ccccgaagac accatcgaga tgcttgcggt
     7681 gcacggcgac gaggcgtggc tcgtccagac cccgcacatc ccgttcaagg tcaacgggtc
     7741 cggcgacgtc accgccgccc tcttcaccgc ccacctgctc gactccggcc gcgccgacga
     7801 tgccctggcc aggacggtct cctccgtcta tgccgtgttg aagcggacct tcgagtccgg
     7861 ccgccgggag ttgcagctgg tgcagagcca ggaggagatc gcccacccgg cgatggagtt
     7921 cacccccgaa cgcgtgcgct gagtcctcga cgtcacacga cgtcgacccg ccgcgccagt
     7981 gggatggtga tcagcgcggc gagtatgccg gcagccacgg tgatcgtgat cggccaggcg
     8041 gtcaggtggt cggcttcccg ggccacgagc g
//
LOCUS       DAOPJG010000155         8053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9495_length_8053_cov_7.738935, whole genome shotgun sequence.
ACCESSION   DAOPJG010000155 DAOPJG010000000
VERSION     DAOPJG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9495_length_8053_cov_7.738935"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKU75_12735"
     CDS             complement(<1..234)
                     /locus_tag="PKU75_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81790.1"
                     /translation="MPDAVPGSPAPAPFGLVVRFELHPRQEAAFDAHEQTEHTRAFLA
                     ERQRFVASFEVAFLGPGSAASADRDRQQRRPSAG"
     gene            complement(317..390)
                     /locus_tag="PKU75_12740"
     tRNA            complement(317..390)
                     /locus_tag="PKU75_12740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Thr,seq:cgt)
     gene            complement(465..1235)
                     /locus_tag="PKU75_12745"
     CDS             complement(465..1235)
                     /locus_tag="PKU75_12745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF81791.1"
                     /translation="MAQSRPLALITGGGTGIGAATATRLAAAGFDVLLAGRRLEPLTS
                     TAARIGAAARHTVLDVTDPEAVGELIGGLDRLDVVVNNAGGALGLAPVAEADAAQWRT
                     MFEVNVLGTLNVVRAALPLLLRSPRATIVDISSTAGEIVYEGGAGYAVAKHGTSVISE
                     TLRLELSGTHVRVVDIRPGMVATDEFALTRFGGDAAAAAKVYADVDRPLVADDVAACV
                     EFAVQLPQHVNIDTLTLRPVAQAAQHKTYRGPIDWHEG"
     gene            1752..3242
                     /locus_tag="PKU75_12750"
     CDS             1752..3242
                     /locus_tag="PKU75_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPF81792.1"
                     /translation="MPCRPASQPRARIRAAQAIGAFAAVTGVVLGGSYAASAEPTGPS
                     QAEVDGARSAVAAAGTQVAALDASYAAASRRLAAVRERSAALGEAANGARWELEKATA
                     AALDARRKSDAAAHTARGARDELREFAAGLYQQDGALGTLGSLLDAGDPQQLLDRAST
                     LEIVSDQQDARLGEALRLSGTAQTLQAAADEAEKARRSAFTRAEQAERIARAEFDLAD
                     AEASRLQRTRAGLAERLAALRDTSVRLERERLDAIAAEAAAQEAAAQEAAQAANEPPT
                     SAPPASGTATPPVATGTSTRPTSSATRPTATSTSPRPTSTSTPPTSTSSSTRPTSTST
                     STRPTSTSTSTRPTSTSTSTRPTTTSSSTPPPTSTSTSTPPPASGAAAAIAYAQAQLG
                     KPYLWGGSGPDAFDCSGLTMMAWRQAGVYLSHYTGAQYAETARVPIDQLQPGDLVFFG
                     SSGESSHHMGLYVGGGQMIEAPYTGAVIRYASIYRSDLVPYGGRPG"
     gene            complement(3270..4232)
                     /locus_tag="PKU75_12755"
     CDS             complement(3270..4232)
                     /locus_tag="PKU75_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HPF81793.1"
                     /translation="MTTDAERAVEGLVRLLTLREVGTAHIAVSDAPETDTEMGDSEAQ
                     VFIGASEKQPHGRVFGGQVLAQGVMAAGLTLDLTPDATPRPIHSLHAYFMRPGDDTQP
                     IRFAVERMMDGNSFSTRRVHAIQHGRPILAMSASFQERAEGMEHQDPMPSAPDPRGLT
                     ELSVALGDLMDRQGIVDERAEHHLARLRKRAIRQYPVGSVDPREPATRSSRYQLWMRA
                     AASLPDDQLLTSAILAYASDFQMLTPSLRNHGVTFFDPRLRVASLDHAMWFHRDASLS
                     DWLLFDLESPSAQSGRALGRGTVWTEDGRLVASLAQEGMTRLKA"
     gene            complement(4268..5455)
                     /locus_tag="PKU75_12760"
     CDS             complement(4268..5455)
                     /locus_tag="PKU75_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HPF81794.1"
                     /translation="MTVEQVWRTEAPHVLAALTRRYGDFGDCEDAVQEALLAAAAQWP
                     REGMPHDPRAWLITVAQRRLIDQVRSESARRRREERYAADALDGAAPVGVDDSLEVLL
                     LCAHPALTKASQVALILRAVSGLTTAQIAAGFLVPEATMAARITRAKKTLAAAGSRFP
                     APAADLSARVGAVRHALALLHTEGHVRSSGEELSDAGFSGEAIRLARSLWRAVPADPE
                     NAGLLALLLLTHARGPARTDAHGDLVPLEAQDRSRWRRDLIAEGVAILEAVLPVGPVG
                     EFQLQAAIAAVHDEAASWAATDWPQIAELYRMLDLVAPGEAVTIGRAVAVARTEGPAA
                     GLALLDPLAGSRNHRVAATRGHLLAELGRTAQARVALEQAARMTPSIPEQRYLNRLLH
                     EMA"
     gene            complement(5465..5857)
                     /locus_tag="PKU75_12765"
     CDS             complement(5465..5857)
                     /locus_tag="PKU75_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPF81795.1"
                     /translation="MKYVILIHSNPQPWGHPTGDFVAEQRDLPAETREQLNADFESVL
                     EEMSARGELIGGEALADPASARLFRWHDGERLATDGPYAEGKEHLAGFFLIDVESPER
                     AEEITRNFGAPGETIELRPAMWPGGDAD"
     gene            6016..6537
                     /locus_tag="PKU75_12770"
     CDS             6016..6537
                     /locus_tag="PKU75_12770"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPF81796.1"
                     /translation="MTETTPMTTDTGELLAWIGRQHHRLLASTAGLTEEQLRAPVVSS
                     GWSVLGLLGHTRDTTVLWLHHIVAGRPVDGFDEEEEVFDNDPQRPAGEVVQDLREACA
                     AAVEQAREIPADAAPGWWPEGAWGGYRQETVRGCLLHLLTELAAHAGHMDIARELTDG
                     GVWDFAVDGVRVP"
     gene            complement(6645..7289)
                     /locus_tag="PKU75_12775"
     CDS             complement(6645..7289)
                     /locus_tag="PKU75_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPF81797.1"
                     /translation="MEFDVTIEIPKGARNKYEVDHESGRIRLDRLLFTSMAYPADYGY
                     VEDSLGEDGDPLDALVLLDEPTWPGCLVRARPIGMFHMRDEAGGDDKILCIPAGDPRK
                     SGITELEHINEFDRLEIQHFFETYKDLEPGKSVEGAHWTGRAEAEECVRQAIQRAHDA
                     GVSTARWRTPAQGNQAIADFAQSRHGHTAEEMQEASRRAEQELATKEHPLSDDR"
     gene            7375..>8053
                     /locus_tag="PKU75_12780"
     CDS             7375..>8053
                     /locus_tag="PKU75_12780"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HPF81798.1"
                     /translation="MRRSATVLAVAALALGGYGAADVYDVVPGILTRDRPTPLPAPAT
                     PGPTPTPVALPTAAAAIPVLAALADDAPVPSTAALKKVLDAAVADPALGPSVGVSIRD
                     AATGKELYARGADAPRVIASAQKILAAVAISSELDPRARMTTEVRMVAPGELVLVAGG
                     DTLLAREAGDPDATAGRVGLRDLAEQVAAAVGEEPVERWTVRLDLTYAAGPGYPDTPT
                     YPTGWAPG"
BASE COUNT         1136 a         2930 c         2849 g         1138 t
ORIGIN      
        1 cccggccgac ggcctgcgct gctgacggtc ccggtcagcg gatgctgctg accccggccc
       61 caggaacgcg acctcaaagg acgcgacgaa gcgctggcgc tcggcgagga aggcgcgcgt
      121 gtgctcggtc tgctcgtgcg cgtcgaaggc cgcctcctgc cggggatgca attcgaagcg
      181 gacgacgaga ccgaacggcg cgggagcggg agaccccggg acagcatcag gcatgctctc
      241 gccgtagcga gatcgcaccg aaacgagcct cacgggacaa caaacgcccc cgaccggtgt
      301 cgagggcgtt tggcggagcc gcttgtcgga atcgaaccga cgacctattc attacgagtg
      361 aatcgctctg gccgactgag ctaaagcggc gggtgcgccg agcatggctg ggtgcgccca
      421 agcagtatac gtatgccgcg gccacgcacc gaatcgacct cggctcagcc ctcgtgccag
      481 tcgatcggtc cgcggtaggt cttgtgctgg gccgcctggg cgaccgggcg gagggtgagg
      541 gtgtcgatat tgacatgttg cggcagctgg acggcgaact ccacgcacgc ggcgacatcg
      601 tcggccacga gcggccggtc gacatcggca tacaccttcg ccgccgcggc cgcgtcgccc
      661 ccgaaccggg tgagcgcgaa ctcgtccgtg gcgaccatgc cgggccggat gtcgacgacc
      721 cgcacgtggg tgccgctcag ttcgagccgc agcgtctcgg agatcaccga ggtgccgtgc
      781 ttggccacgg catagccggc gccgccctcg tagacgatct cgccggcggt cgaggagatg
      841 tcgacgatcg tcgcccgggg cgagcgcagc agcagcggca acgcggcccg caccacgttc
      901 agcgttccga gcacattgac ctcgaacatc gtgcgccact gcgccgcgtc cgcctcggcg
      961 accggcgcga gcccgagcgc gccgccggcg ttgttgacca cgacgtcgag ccggtccagg
     1021 ccgccgatga gctcgccgac ggcctcgggg tcggtgacgt cgaggacggt atgccgtgcc
     1081 gccgcgccga tccgggccgc agtgctggtc aggggctcga ggcggcggcc ggcgaggagg
     1141 acgtcgaagc cggctgcggc gagtcgggtc gccgtggcgg caccgatgcc ggtgccgcca
     1201 ccggtgatga gggcgagcgg acgggactgg gccatgactg ccatcgtaag ggtggtgtcg
     1261 cgggcctccc attcatgata aagcccggcc acattgtttt ctggacgccc ggtcaatccc
     1321 tgtgcgcgcc agcttttacc aagccttatg gacaaactga ccaaggtcga tgaggtagcg
     1381 tcagaagcac cgccaatgag ccgacgcggg gtgggagcca acctccgggt tgtccccgca
     1441 gtcgccgggc ggtaagacgg gggcgctgat gtcagcatca gcgcccccgt cgtcttcccc
     1501 ctcttccgac cgttcgttat ggattcgtta ggcggcgggg accgcggtgc cactcgggcg
     1561 cgcgacctgc ggaaaaacat ccgtgtcatt cgctggccaa ggccctccgc ggacttgaac
     1621 gggcgcctga ggtgtgacgg atgtgacggt gggaatcggg ctcagtaccc tgaactgcgc
     1681 aaacagcgtt tggccccccg aatcggccac cgtccgccat cggtcgccgg agtttctcag
     1741 gagcgtcgtc catgccgtgc aggccagcct cacagccgcg cgcccggatc cgggcggccc
     1801 aggccatcgg agcgttcgcc gcggtcaccg gggtcgtcct cggcgggtcg tatgccgcca
     1861 gcgccgagcc gaccggtccg agccaggccg aggtggacgg cgcccgcagc gccgtggccg
     1921 cggccgggac ccaggtcgcc gcgctcgatg cgtcgtatgc cgccgcgtcc cgccgcctgg
     1981 ccgccgttcg tgagcgctcc gccgccctcg gcgaagccgc caacggcgcc cgctgggagt
     2041 tggagaaggc gaccgctgcg gcactcgacg cccgccgcaa gtcggacgct gctgcccata
     2101 cggcccgcgg ggcgcgcgac gagttgcgcg agttcgccgc cggtctgtac cagcaggacg
     2161 gggcgctcgg caccctcggt tcgctgctcg acgccggcga cccccagcaa ctcctcgacc
     2221 gcgccagcac gctcgagatc gtctccgacc aacaggacgc ccgcctcggc gaggcgctgc
     2281 ggctgagcgg gaccgctcag accctgcaag ccgccgccga cgaggccgag aaggcccggc
     2341 gctccgcctt cacccgcgcc gaacaagccg aacgcatcgc ccgggccgag ttcgacctgg
     2401 ccgacgccga agccagccgc ctccaacgca cccgcgccgg gctcgccgaa cgacttgccg
     2461 cgctgcgcga cacctccgtg cggctcgagc gcgagcggct cgatgcgatc gccgccgagg
     2521 cggccgcgca ggaagctgcc gcgcaagagg ccgcccaggc cgcgaacgag ccgccgacct
     2581 ccgccccacc ggcgagcggc accgcaaccc caccggtcgc cacgggcacc agcactcggc
     2641 ccacgtcctc cgcgacccgc cccacggcca cgtccacgag cccgcggccg acgtcgacct
     2701 cgaccccgcc cacgtcgacg tcctcctcca cgcgcccgac gtccacctcc acctcgaccc
     2761 gcccgacgtc cacctcgacg tccacccgcc cgacgtcgac ctccacgtcc acccgcccca
     2821 cgacgacctc gagcagcacg ccgccgccga cctccacatc gacctcgacc ccgccgccgg
     2881 ccagcggcgc cgcggccgcc atcgcctatg cgcaggccca actcggcaag ccgtatctgt
     2941 ggggcgggtc gggaccggac gccttcgact gctccgggct gacgatgatg gcctggcgcc
     3001 aggccggggt ctacctctcc cactacaccg gcgcgcagta tgccgagacc gcccgggtcc
     3061 cgatcgacca gttgcagccc ggtgatctgg tcttcttcgg ctccagcggc gagagcagtc
     3121 accacatggg gctgtatgtc ggtggcggcc agatgatcga agctccgtac accggggcgg
     3181 tcatccggta cgcctccatt tatcgcagcg acctggtccc ctacggcggc cgcccgggct
     3241 gacgcgcacc acgggcgcac cgatccgggt cacgccttca ggcgcgtcat cccctcctga
     3301 gcgaggctcg ccacgagccg cccatcctcg gtccagaccg tcccgcgccc cagggcgcga
     3361 ccgctctgtg cggacgggct ctcgaggtcg aagagcagcc agtcgctcag cgaagcgtcc
     3421 cggtggaacc acatcgcgtg atcaaggctg gcaacccgca gccgggggtc gaagaaggtg
     3481 acgccgtggt tgcgcagcga cggcgtgagc atctggaagt cggaggcgta cgcgaggatg
     3541 gcggaggtca ggagctgatc gtccgggagc gacgcggccg cccgcatcca gagctgatag
     3601 cggctcgacc gggtcgccgg ttcgcgcgga tcgaccgagc cgacggggta ttgccggatg
     3661 gctcgcttgc gcagccgcgc gaggtggtgc tccgcgcgtt cgtcgacgat gccctgccgg
     3721 tccatcaggt cgcccaaggc gacgctcaac tcggtcagcc cgcgcgggtc gggtgccgag
     3781 ggcatcggat cctggtgttc catgccctcg gcccgctcct ggaaggaggc ggacatcgcc
     3841 aggatcggcc ggccgtgctg gatcgcatgc acgcgccggg tgctgaagga gttgccgtcc
     3901 atcatccgct cgaccgcgaa gcggatcggc tgggtgtcgt cgccgggtcg catgaaatag
     3961 gcgtgcagcg agtggatggg ccgaggcgtg gcatccgggg tcaggtccag ggtcaggcct
     4021 gccgccatga cgccctgcgc gagcacctgc ccgccgaata cccgcccgtg cggctgcttc
     4081 tcgctggcgc cgatgaaaac ctgggcctcg ctgtcgccca tctcggtgtc cgtttccggg
     4141 gcgtcgctga cggcgatatg agcggtgccg acctccctca gggtcagcag cctgacgagg
     4201 ccctcgacgg cgcgttcggc atcggtggtc acgagcacgg cagcttagcc agtcggggcg
     4261 gcccggttca cgccatctcg tggaggagcc ggttcagata gcgctgctcc gggatcgatg
     4321 gggtcatgcg ggccgcctgc tccaaggcga cgcgcgcctg cgcggtgcga cccaactccg
     4381 cgagcaagtg cccgcgcgtg gcggcgaccc ggtggttgcg ggagccggcg agcgggtcga
     4441 gcagggccag cccggccgca ggtccctcgg tgcgcgccac cgccaccgcc cgaccgatcg
     4501 tcaccgcctc gccgggggcg acgaggtcga gcatccgata gagctcggcg atctgcggcc
     4561 agtccgtggc cgcccacgag gcggcctcgt cgtggaccgc cgcaatggcc gcctgcaatt
     4621 ggaactcacc gaccggaccg accgggagca ccgcctccag gatcgccacg ccctccgcga
     4681 tcaggtcgcg acgccagcgc gaacggtctt gtgcctcgag aggaaccagg tcgccatggg
     4741 cgtcggtgcg cgcgggcccg cgagcgtgcg tgagcagcag cagggcgagc agtccggcat
     4801 tttcggggtc ggcgggcacg gcgcgccaca acgagcgggc gagccggatc gcctcgccgc
     4861 tgaaccccgc gtcgctcaac tcctcaccgg aggagcgcac atgcccctcg gtatgcagca
     4921 gcgccagggc gtgccgcacg gcaccgacgc gtgcggacag gtcggcggcc ggagcgggga
     4981 accggctccc cgccgcggcc agcgtcttct tggcgcgcgt gatccgggcg gccatggtcg
     5041 cctcggggac caggaagccg gcggcgatct gcgccgtcgt cagcccggag accgctcgca
     5101 ggatgagcgc gacctgcgag gccttggtca gcgcggggtg ggcgcagagc aatagcacct
     5161 cgaggctgtc gtcgacaccg actggagcgg cgccgtcgag ggcgtccgcg gcataccgct
     5221 cctcgcgccg ccggcgcgcc gactcgctgc gcacctgatc gatcagccgc cgctgcgcca
     5281 cggtgatcaa ccaggcgcgc ggatcgtgcg gcataccctc ccgcggccac tgggcggcgg
     5341 cggcgagcaa cgcctcctgg accgcgtcct cgcaatcgcc gaagtcgccg tagcggcggg
     5401 tcaacgcggc gagcacgtgt ggcgcctcgg tgcgccacac ctgctcgacc gtcatcgcgc
     5461 gccctcagtc ggcgtcgccg ccaggccaca tggccggccg caactcgatg gtctcgcccg
     5521 gcgcgccgaa gttgcgggtg atctcctcgg cgcgctccgg cgactcgacg tcgatgagga
     5581 agaacccggc caggtgctcc ttgccctcgg catacggccc gtcggtcgcc aggcgctcgc
     5641 cgtcgtgcca gcggaacagg cgggcgctcg cgggatcggc cagggcctca ccgccgatca
     5701 gctcgccgcg ggcgctcatc tcctcaagca ccgactcgaa gtcggcgttc agttgctcac
     5761 gcgtctcggc cggcaggtcc cgctgctccg cgacgaagtc gccggtcggg tggccccaag
     5821 gctgggggtt ggagtggatc aagatgacgt acttcactgc tcgttttctc cttcgactcg
     5881 cgccggcgcg gtgccggtcg ggcgtaggtc gaagccagat ccggcacatc gacacgtgcg
     5941 gtcatgtcga tccggcgttg tcggctccga ccttcgggtg tccagcacca ccgacgacaa
     6001 cgttcggagc acatcatgac cgagaccacc cccatgacca ccgacaccgg cgaactgctg
     6061 gcgtggatcg gccggcaaca ccatcgcctg ctcgcgagca cggccggcct gaccgaggag
     6121 cagctgcgcg cgccggtcgt gtcctcgggc tggtccgtcc tcggcctgct cggccacacc
     6181 cgcgacacca ccgtcttgtg gctgcaccac atcgtcgccg gccgccccgt cgacggcttc
     6241 gacgaggagg aggaggtctt cgacaacgac ccgcagcgcc ccgccggcga ggtggtgcag
     6301 gatctccggg aggcctgcgc ggccgcggtc gagcaggccc gcgagatccc ggcggacgcg
     6361 gcacccgggt ggtggcccga gggcgcttgg ggcggctatc ggcaagagac cgtgcgcggc
     6421 tgcctgctgc acctgctcac ggaactggcc gcgcacgcgg ggcacatgga catcgcccgg
     6481 gaactgaccg acggcggcgt gtgggacttc gccgtcgacg gggtccgggt cccgtgaccg
     6541 cgaggtcagt ccggcggtgc tgaggcccag ccgaccgtca ggttgcacag acggaggcga
     6601 ccgccgcggc atcgcgacgg tcgcctctcg gatcgtgacc ggcttcagcg gtcgtcggac
     6661 agcgggtgct ccttggtcgc cagttcctgc tcggcccggc gggaggcttc ctgcatctcc
     6721 tctgcggtgt ggccgtggcg ggactgggcg aagtcggcga tggcctggtt gccctgcgcc
     6781 ggggtgcgcc agcgggccgt ggacacgccg gcgtcgtggg cgcgctggat ggcctggcgc
     6841 acgcattcct cggcttcggc ccggccggtc cagtgggcgc cctcgaccga cttgcccggt
     6901 tcgaggtcct tgtaggtctc gaagaagtgc tggatctcca gccggtcgaa ctcgttgatg
     6961 tgctccagct cggtgatgcc gctcttgcga gggtcaccgg cggggatgca caggatcttg
     7021 tcgtcgccgc cggcctcgtc gcgcatatgg aacatcccga tcggccgcgc ccggacgagg
     7081 cagccgggcc acgtcggctc gtcgaggagc acgagcgcgt cgagcgggtc gccgtcctcg
     7141 ccgaggctgt cctcgacata gccgtagtcg gccgggtagg ccatggaagt gaacagcagc
     7201 cggtcgagcc ggattcgtcc gctctcgtga tccacctcgt acttgttgcg cgcgcccttg
     7261 gggatctcaa tcgtgacgtc gaactccacc ctgtctctcc tcatcgctcg aatgacctac
     7321 tctgctcgct aagtcttgcg catccggcgc ggtgtggtgt ggagggggta tgccgtgcgt
     7381 cgctcggcga ccgtcctcgc cgtcgccgcg ctggcgctgg gcggctacgg cgccgccgac
     7441 gtctacgacg tcgtgcccgg catcctcact cgtgaccggc cgacgccgct accggccccg
     7501 gccacgcccg gccccacgcc cactccggtc gcgctgccca ctgcagcggc cgccataccg
     7561 gtgcttgcgg ccctcgccga cgacgcgccg gtgccgtcga ctgcggcgct gaagaaggtc
     7621 ctggacgcgg cggtcgccga cccggccctc ggtcccagcg tcggcgtcag catccgggac
     7681 gccgcgaccg gcaaggagct gtatgcccgt ggcgccgacg cgccgcgcgt catcgcctcg
     7741 gcgcagaaga tccttgcggc agtggcgatt tcgtccgagc tcgatccgcg cgcgcggatg
     7801 acgaccgagg tgcggatggt cgcgccgggg gagctggtcc tggtcgccgg gggcgacacc
     7861 ttgctggccc gtgaggccgg tgacccggac gcgactgccg gccgggtggg gttgcgcgat
     7921 ctggccgagc aggtcgcggc ggccgtgggg gaggagccgg tcgagcggtg gacggtgcgc
     7981 ctggacctga cgtatgccgc cgggcccggc taccccgaca cgcccaccta tccgacgggt
     8041 tgggctcccg gtg
//
LOCUS       DAOPJG010000156         8002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9581_length_8002_cov_8.110104, whole genome shotgun sequence.
ACCESSION   DAOPJG010000156 DAOPJG010000000
VERSION     DAOPJG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9581_length_8002_cov_8.110104"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKU75_12785"
     CDS             complement(<1..444)
                     /locus_tag="PKU75_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF81799.1"
                     /translation="MPEFIYVFSKARKAHGDKVILDDVTLSFLPGAKIGVVGPNGAGK
                     STVLKIMAGLEQASNGEARLTPGYSVGILMQEPKLDETKTVLGNVEEGAGEIKKALDR
                     YNEISELMSDPDADFDALMTEMGQLQEKIDSADAWDLDSQLEQAMD"
     gene            658..1344
                     /locus_tag="PKU75_12790"
     CDS             658..1344
                     /locus_tag="PKU75_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPF81800.1"
                     /translation="MQELRVTISGNVVADPELRIGSTGNPFTTFRVAVNITKRTDAGV
                     YQEVRTEYYRVTAFGALGHNAMGSLKKGQGVLVYGRFKTETYPRQDGSEGASIEITAD
                     ALGHDLTFGWADYHKGRKPAEDSSDPRNDPAVRAARQEDYARDQHARDQQRSGWGSGA
                     HNWNSDYVTTDAGTVDPRTGELSSGADGYAADSGTGEAAAPVDLGQGFPPDLDEAEED
                     VESADDKALV"
     gene            complement(1363..3012)
                     /locus_tag="PKU75_12795"
     CDS             complement(1363..3012)
                     /locus_tag="PKU75_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPF81801.1"
                     /translation="MSPLFGRGDKGVSAEDLTKHADALRSAVDAGGAELDPDQVARAR
                     AIATKVSERMGRSGSHTVVAFAGATGSGKSSLFNAVIGAEVAKPGARRPTTSTPTAAI
                     WGDDPAGPLLDWLVVPNRHQVPDSEGSALDGLVLLDLPDFDSREAAHRAEAERVIGLA
                     DVFVWVTDPQKYADARLHDDYVAAMKNYGAVTIVILNQADRLTPDQQRQCLADLERLL
                     HRDGLSEVTVLATSARTGLGVPELRDKLSDAVSGARAARTRLDADVRSAATDLRAGVA
                     ATEADVQGPHRRELVSALSRAAGVPTVLEAVRRDYQRDAVAATGWPFTRWITKFRPDP
                     LKRLRLDPKEREALTGEGRRALGRSSIPVANPAARAAVDLAARRLADAAGAGLPHRWA
                     EAVSAAAQPSQDRLADELDQAVMATPLRSNRPIWWSLLSILQILLAIAVVVGLVWTLV
                     EVFFGAQDLVDLPMWHVGPFGLPLVLLVGGLLLGWLLALLSRWFAGIGARRRAAGTGK
                     RLDEAIGEVADRVIVAPVSDVLARHRQTRIQLETAAAGGKR"
     gene            complement(3009..4712)
                     /locus_tag="PKU75_12800"
     CDS             complement(3009..4712)
                     /locus_tag="PKU75_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPF81802.1"
                     /translation="MTATMDDPAKLLAAVREFREQVAASVLPLDLPSTAEAKDNRAAL
                     LRQLDDYILPRLSSINAPLLAVIGGSTGAGKSTLVNSLLRRQVSRAGVIRPTTTSPVL
                     IHHPDDGHWFTDQRVLPELSRVTGDGDDAEGTVRLAESRELPAGLALLDAPDIDSVVS
                     ANRGLSRQLLSAADLWLFVTTAARYADAVPWDLLRQASDRGTAVAIILDRVPPEVMAE
                     VRPHLVDMLRAQGLGTAPVFPIPETTLDGNGLLPETDVARLRSWLTDLANDATAREVV
                     VRQTLTGALASLDSRSEELVTASRTQQRVTADLTDIVNRAFDDSRRGVENGMSDGQLL
                     RGEVLARWQDFVGTGEFFRKVESGVSKLRDRITGAFRGHANAPADVEEALHVGVAQLM
                     EANAATAAAQVAREWRALPAGAEILHAHPELARPSSDLGPRVDAAVRTWQDEVLDLVR
                     SEGQDRRTNARVAAYGINGIGLFLMLVTFAHTGGLTGAEAGIAGGTSVLAQRVLEAIF
                     GDQAVRTMAAKARAALLRRADEVIEAERTRFLDAVGARTVAADQTERLSRALDAVQAA
                     R"
     gene            complement(4745..4818)
                     /locus_tag="PKU75_12805"
     tRNA            complement(4745..4818)
                     /locus_tag="PKU75_12805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4782..4784),aa:Arg,seq:tct)
     gene            complement(4855..6051)
                     /locus_tag="PKU75_12810"
     CDS             complement(4855..6051)
                     /locus_tag="PKU75_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPF81803.1"
                     /translation="MTTPAVPADLAGDIIALRRDLHRHPELGLHNPATQQRIVDALAG
                     LPLEITRGRDCTSVVAVLRGGAGSGRAVLLRGDMDALPVTEDLPLDFVSQEPGLMHAC
                     GHDLHVAGLVGAARLLCARRAELAGDVIFMFQPGEEGPGGAEVMLREGLLDAAGVPVV
                     AAYALHVAAAGERLGTWFGRPGPLMAAADEAVIRIVGEGGHGSQPHLAKDPVPVACEI
                     VLAMQLMVTRQFNAMEPMVLTVGKIAGGTKDNIIPDDAVLELTLRTFDDEQRRTAHTA
                     IARVAEGIASAHGLTAEVTWLMGYPVTVNDETEYAFLRATVEEIFGAERYVDMPHPEA
                     GAEDFAFVAGRVPSAYVFLSACPADEPGSAPDNHSPRASFDDAVVPDGALLLAELAMR
                     RLADPR"
     gene            complement(6048..7259)
                     /locus_tag="PKU75_12815"
     CDS             complement(6048..7259)
                     /locus_tag="PKU75_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HPF81804.1"
                     /translation="MSWQQAPVAPPSQAPGHLAEPTAYDPGYQPQGGGHTSRRALRGW
                     LLTGLIIAGFTIAALALAAYFQASIGLMTALLALLLAALPLGVVVPTFLWLDRFEAEP
                     TKYLVFAFLWGALVASVLAALFNTGAMLVIQSASDPTSAMATTAVVVAPIVEETLKGL
                     LVLIVWRLRRREFDGLIDGLVYAGITAAGFAFTENIQYLGQGYAAGGGEVLAATFVGR
                     GIMSPFAHPMFTCLTGIGIGIAATARSTGVRIVAPITGWVLAMLAHGLWNLSAVTGGA
                     GLLVGYFLVGVPVFCAYVGLVIWARRREGRLIGRHLSPYADAGWLSPSEVIMLSSMGH
                     RREARVWAKTNAGSTGLKAMRQFQDAASELGLLRQRMYHQAADAHALTAERKLLDELT
                     VARRTFIGSGR"
     gene            7329..7952
                     /gene="orn"
                     /locus_tag="PKU75_12820"
     CDS             7329..7952
                     /gene="orn"
                     /locus_tag="PKU75_12820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HPF81805.1"
                     /translation="MSKTATDRAAKDRIVWIDCEMTGLSLEHDALIEVAALVTDFDLN
                     QLGDGVDLVIKPPQSALEQMNDFVTRMHTDSGLIDELDGGISLQEASARVLDYVRTWV
                     PEARKAPLGGNSVATDRGFLARDLPELDAWLHYRIIDVSSIKELSRRWYPRAYINSPE
                     KKGGHRALADIRESIAELRYYREAVFVPQPGPDTPTAKAIAAHYQLD"
BASE COUNT         1214 a         2775 c         2901 g         1112 t
ORIGIN      
        1 gtccatggcc tgctcgagtt gcgagtcgag atcccaggcg tcggcggagt cgattttctc
       61 ctgcaactgc cccatctcgg tcatgagcgc atcgaagtcg gcatcgggat ccgacatcag
      121 ctcggagatc tcgttatagc gatcgagcgc cttcttgatc tcccccgcgc cctcctcgac
      181 attgcccagg acggtcttgg tctcgtcgag cttcggctcc tgcatgagga tgccgacgct
      241 gtatcccggg gtcagtcggg cctcgccgtt ggaggcctgc tccaggccgg ccatgatctt
      301 caggaccgtc gacttgccgg ccccgttggg tccgacgacg ccgatcttgg cgccgggcag
      361 gaaggacaga gtgacgtcat cgaggatgac tttgtctccg tgcgccttgc gcgccttgga
      421 gaagacatag atgaactcgg gcatgccgcc aaggctaact gcggccctgc cgtgctccga
      481 atcggcgcca ccccgcacgc ctggttgtcc acagccaaga tcgcaacttc ccgtcgtcca
      541 cagggttttc ggcgaccctt ggccgggttc gcgcggcacg gcaggctggc accgaggctg
      601 cgcgcagggc gtggccagac atcatcagca acacacctac accttggggg tgcagcaatg
      661 caggaacttc gcgtcaccat cagcggcaat gtcgtcgccg accccgaatt gcggatcggc
      721 agcacgggaa accccttcac gacattccgg gtcgccgtca acatcaccaa gcgcaccgac
      781 gccggggtct accaggaggt ccggaccgag tactaccgag tgaccgcgtt cggggcgctc
      841 gggcacaacg cgatgggcag cctgaagaag gggcaaggcg tgctcgtcta cggccgcttc
      901 aagaccgaga cctatccgcg acaggacggc tctgaagggg cgagcatcga gatcacggcg
      961 gatgcgctcg ggcacgacct gaccttcggc tgggccgact atcacaaggg gcgcaagccg
     1021 gccgaggact cgagcgaccc gcgcaacgac ccggccgtcc gcgcggctcg gcaggaggac
     1081 tatgcccgcg accagcacgc tcgcgaccag caacgcagcg gctggggcag cggtgcccac
     1141 aactggaact ccgactacgt caccaccgac gccggcaccg tcgatccccg gacgggtgag
     1201 ctcagctccg gagcggacgg ctacgcggcc gattccggca cgggcgaggc ggctgcgccc
     1261 gtggacctgg gccaaggatt cccgccggat ctcgacgaag ccgaggagga cgtcgagtcg
     1321 gcggatgaca aggctctggt ctgagcgtcg cgggcccgtg ggctaccgct tgccgcccgc
     1381 agcggcggtc tcgagctgga tccgggtctg ccggtgccgg gcgagcacgt ccgagaccgg
     1441 cgccacgatg acccggtccg cgacctcccc gatcgcctcg tcgagccgct tgcccgtccc
     1501 ggcagcacgg cgccgggcac cgatgccggc gaaccacctg gacagcagcg cgaggagcca
     1561 gccgagcagg aggcctccga cgagcagcac cagcgggagc ccgaaggggc cgacgtgcca
     1621 catcggaagg tccacgaggt cctgggcgcc gaagaacacc tcgaccaggg tccagaccaa
     1681 cccgacgacc accgcgatgg ccagcaggat ctgcaggatg ctcagcaagc tccaccagat
     1741 cggccggttg ctccgcagcg gagtggccat cacggcttgg tcgagttcgt cggcgaggcg
     1801 gtcctgcgag ggctgcgcgg cggcggagac ggcctcggcc cagcgatggg gcaggccggc
     1861 gccggcagcg tcggcgagcc gccgggcggc caggtcgact gcggcccggg cggcggggtt
     1921 ggccaccggg atcgaggacc gcccaagtgc ccggcggccc tcgccggtga gggcctcacg
     1981 ttccttcggg tccagccgca accgcttgag cgggtcgggc cggaacttgg tgatccaccg
     2041 tgtgaagggc cagccggtgg cggcgacggc gtcacgctgg tagtcgcggc ggacggcctc
     2101 gagcacggtc ggcacgccgg cggcgcgcga aagggcgctg accagttcgc gccggtgcgg
     2161 gccctggacg tcggcctcgg tcgcggcgac gccggcccgc agatcggtgg cggcggagcg
     2221 gacgtcggcg tccaggcggg tgcgagcggc ccgggcacca gagaccgcgt cggagagctt
     2281 gtcgcgcagt tcgggcacgc ccagtccggt gcgggccgag gtggcgagca ccgtgacctc
     2341 ggacaggccg tcgcgatgca gcaggcgctc caggtccgcg aggcactggc gctgctggtc
     2401 gggggtgagc cggtcggctt ggttgaggat gacgatcgtc accgcgccgt agttcttcat
     2461 cgccgcgacg tagtcgtcgt gcaggcgggc gtcggcgtac ttctgcgggt cggtcaccca
     2521 gacgaagaca tcggcgagcc cgatcacgcg ttcggcctcg gcccggtgcg cggcctcgcg
     2581 ggagtcgaag tccggcaggt cgagcagcac gagtccgtcc agggcgctgc cctcggagtc
     2641 cggcacctgg tggcggttgg gcacgacgag ccagtccagc agcggtccgg ccgggtcgtc
     2701 gccccagatc gcggcggtcg gggtcgaggt cgtggggcgg cgggcgccgg gcttggcgac
     2761 ctcggcaccg atgacggcgt tgaagaggct ggatttgccg ctgcccgtgg ccccggcgaa
     2821 ggcgacgacc gtatggctgc cgctgcgccc catccgctcg ctcaccttcg tggcgatggc
     2881 ccgtgcgcgg gcgacctggt ccgggtccag ttcggcgccg ccggcgtcga cggcggagcg
     2941 cagcgcgtcg gcgtgcttgg tgagatcctc ggcgctcacg cccttgtcgc cgcggccgaa
     3001 cagggggctc atcgagcggc ctgcacggcg tccagggccc tggagaggcg ttcggtctga
     3061 tcggcggcga cggtccgggc gcccacggcg tcgaggaagc gggtgcgctc ggcctcgatg
     3121 acctcgtcgg cccgtcgcag gagggcggcg cgggccttgg ccgccatggt ccgcacggcc
     3181 tggtccccga agatggcctc cagcacccgc tgggcaagga ccgaggtgcc gccggcgatg
     3241 ccggcctccg cgccggtcag cccgccggtg tgggcgaagg tgacgagcat caggaacagg
     3301 ccgatgccgt tgatgccgta tgccgcgacc cgcgcgttgg tgcgccggtc ctggccttcg
     3361 ctgcgcacca ggtcgaggac ctcgtcctgc caggtccgca ccgcggcgtc cacccggggg
     3421 ccgaggtcgc tgctcggcct ggccagttcc gggtgggcgt gcaggatctc ggcaccggcc
     3481 ggcagggccc gccattcgcg ggccacttga gcggccgccg tcgcggcgtt ggcctccatc
     3541 agctgggcga ccccgacgtg cagcgcctcc tcgacatccg ccggcgcgtt ggcgtgccca
     3601 cggaacgcgc cggtgatcct gtcgcgcagc ttcgagaccc cggactcgac cttgcggaag
     3661 aactcgccgg tcccgacgaa gtcctgccac cgcgcgagca cctcgccgcg cagcagttgc
     3721 ccgtcgctca tcccgttctc gacacctcgg cgggagtcgt cgaaggcgcg gttgacgata
     3781 tcggtcaggt cagcggtgac gcgttgctgg gtgcggctgg cggtgacgag ttcctcggac
     3841 cgggagtcga gggaggcgag ggccccggtc agggtctggc ggacgacgac ctcccgggcg
     3901 gtcgcgtcat tggccaggtc ggtcagccac gaccgcagcc gcgccacgtc ggtctcgggc
     3961 aacagcccgt tgccgtcgag ggtggtctcc ggaatcggga aaacgggtgc cgtgcccagg
     4021 ccctgggcgc gcagcatgtc caccaggtgc gggcgcacct ccgccatgac ctccggcggc
     4081 acccggtcca ggatgatcgc cacggccgtg ccgcgatcgg atgcctggcg cagcagatcc
     4141 caggggacgg catcggcata ccgtgccgcg gtggtgacga agagccacag gtccgcggcc
     4201 gagagcaact gccgcgacaa cccgcggttg gccgagacga cggagtcgat gtccggcgcg
     4261 tccagcagcg cgagaccggc gggcagttcg cgcgactcgg ccagccgcac ggtcccctcg
     4321 gcgtcgtccc cgtcgccggt gacccggctc agttcgggca gcacccgctg gtcggtgaac
     4381 cagtgcccgt cgtcggggtg gtggatcagc accgggctgg tcgtcgtcgg ccggatcact
     4441 ccggcgcggc tgacctgccg gcgcagcagc gagttcacga gcgtcgactt gcccgcgccc
     4501 gtcgacccgc cgatgacggc gagcagcggc gcgttgatcg aggacaggcg gggcaggatg
     4561 tagtcgtcga gctggcgcag cagcgccgcc cggttgtcct tggcctcggc agtgctcggc
     4621 aggtccagcg gcagcacgct ggccgccacc tgctcccgga attcgcgaac ggcggccagg
     4681 agcttggccg ggtcgtccat ggtcgcggtc acgagggtca accttagacg agaggaatcc
     4741 gcagcgcccc cggcaggact cgaacctgcg acctcgggat tagaaggccc ttgctctatc
     4801 cagctgagct acgggggctg gcgaagaaca agagtagatc agcccgagca gtgactatct
     4861 ggggtcggca aggcggcgca tcgcgagttc cgccagcagc aaggccccgt cgggcaccac
     4921 cgcgtcgtcg aagctggccc gcggggagtg gttgtcgggc gcgctgcccg gttcgtccgc
     4981 cgggcaggcc gagaggaaga cataggccga cggcacccgc cccgcgacga acgcgaagtc
     5041 ctccgcaccc gcctcggggt gcggcatgtc gacataccgc tcggcgccga agatctcctc
     5101 gacggtggcc cgcaggaagg cgtactcggt ctcgtcgttg acggtgaccg gatagcccat
     5161 cagccacgtg acctccgcgg tgaggccgtg ggcggaggcg atgccctcgg ccacccgggc
     5221 gatggcggta tgggcagtgc gccgttgctc gtcgtcgaag gtgcgcagcg tcagttcgag
     5281 gaccgcgtcg tcggggatga tgttgtcctt ggtgccgccg gcgatcttgc cgacggtcag
     5341 caccatcggc tccatcgcgt tgaactggcg ggtgaccatc agctgcatcg cgagcacgat
     5401 ctcgcaggcg acgggcacgg gatccttcgc caggtgcggc tgcgagccgt gtccgccctc
     5461 gccgacgatc cggatcacgg cctcgtcggc cgccgccatg agcgggcccg gccgcccgaa
     5521 ccaggtgccg agccgctccc ccgcggccgc cacgtgcagc gcgtatgccg cgacgaccgg
     5581 cacccccgcg gcgtccagga gcccttcgcg caacatgacc tcggccccgc ccggtccctc
     5641 ctcgccgggc tggaacatga agatgacgtc gccggccagc tccgcccggc gcgcgcacag
     5701 cagtctggcg gcgccgacca agccggcgac atgcaggtcg tgcccgcacg cgtgcatcag
     5761 gcccggctcc tgagatacga agtccagcgg caggtcctcg gtgacgggga gggcgtccat
     5821 gtcgccgcgc agcagcaccg cccgccccga ccccgcgcca ccacgcagca cggccaccac
     5881 ggacgtgcag tcccggccgc gggtgatctc cagcggcaga ccggccagcg cgtcgacgat
     5941 ccgctgctgc gtggccggat tgtgcaggcc gagttcgggg tgccggtgca ggtcccggcg
     6001 taaggcgatg atgtcgccgg cgaggtcggc cggcacggcg ggcgtggtca tcgtccgctc
     6061 ccgatgaagg tgcggcgggc cacggtgagt tcgtcgagca gtttgcgctc ggcggtcagc
     6121 gcgtgcgcgt cggcggcctg gtggtacatc cgttgccgca gcaggcccag ttccgaggcg
     6181 gcatcctgga actggcgcat cgccttcagg ccggtggagc cggcgttggt cttggcccac
     6241 acccgggcct cgcggcggtg acccatcgag gagagcatga tgacctcgct cgggctcagc
     6301 cagccggcat cggcatacgg gctcaggtgg cgcccgatca ggcgcccctc gcgccggcgc
     6361 gcccagatga ccagacccac atacgcgcag aacaccggca ccccgacgag gaagtagccg
     6421 acgagcagcc cggcgccgcc ggtgacggcg gacaggttcc acagcccgtg ggcgagcatc
     6481 gcgagcaccc agccggtgat cggggcgacg atccgcaccc cggtgctgcg ggcggtggcg
     6541 gcgatgccga tcccgatgcc cgtcaggcag gtgaacatcg ggtgcgcgaa cgggctcatg
     6601 atgccgcgcc ccacgaacgt ggcggcgagc acttcgccgc cccccgcggc atacccctgc
     6661 ccgaggtatt ggatgttctc ggtgaacgcg aacccggcgg ccgtgatccc ggcatacacc
     6721 agcccgtcga tcagcccgtc gaactcgcgc cgccgcagcc gccacacgat gaggacgagc
     6781 agccccttga gggtctcctc gacgatcggg gcgacgacga cggctgtcgt ggccatcgcc
     6841 gacgtggggt cgctggccga ctggatgacg agcatcgccc ccgtgttgaa cagggcggcg
     6901 agcaccgatg cgaccagcgc gccccagagg aatgcgaaga cgaggtactt ggtcggctcg
     6961 gcctcgaacc ggtccagcca caggaaggtc ggcacgacga ccccgagcgg cagcgcggcg
     7021 agcaggagcg cgagcagcgc cgtcatcagt ccgatcgagg cttggaagta tgccgcgagc
     7081 gccagggcgg cgatcgtgaa accggcgatg atcaagccgg tgagcagcca gccgcgaagg
     7141 gcgcggcggg aggtgtgccc gcccccttgg ggttgatagc ccgggtcgta tgcggtcggc
     7201 tcggccaggt gcccaggcgc ctgggagggc ggcgcgaccg gcgcctgctg ccatgacatg
     7261 ccccagaggc tagttcacgc gcatgcgtca cgcggtgcgg ccgtaaggtt gtccgcgtga
     7321 cagaccggat atcgaagacg gccaccgacc gagcggcgaa ggatcgcatc gtgtggatcg
     7381 actgcgagat gacgggcctg tccctcgagc acgacgcgct gatcgaggtc gctgcgctcg
     7441 tgaccgactt cgatctcaac cagctcggcg acggcgtgga cctggtcatc aagcccccgc
     7501 agtcggcgct ggagcagatg aacgacttcg tcacccggat gcacaccgac tccggcctga
     7561 tcgacgagct cgacggcggc atcagcctgc aggaggcgag tgcgcgggtg ctggattacg
     7621 tgcgcacctg ggttccggag gcgcgcaagg cgcccctggg cggcaacagc gtcgccaccg
     7681 atcgcgggtt cctcgcccgg gacctgcccg agctggacgc gtggctgcac taccgcatca
     7741 tcgacgtctc ctccatcaag gaactctcgc ggcgctggta cccgcgcgct tacatcaact
     7801 cgcccgagaa gaagggcggc caccgggcgc tggcggacat ccgcgagtcg atcgcggaac
     7861 tgcgctacta ccgcgaagcg gtcttcgtgc cgcaacccgg accggacacc ccgacggcga
     7921 aggccatcgc cgcccactac caactcgact agatgattga ttccaaggtg actggtgtgg
     7981 cgtatgggac tcggggggtg ag
//
LOCUS       DAOPJG010000157         7966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9647_length_7966_cov_8.836430, whole genome shotgun sequence.
ACCESSION   DAOPJG010000157 DAOPJG010000000
VERSION     DAOPJG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9647_length_7966_cov_8.836430"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PKU75_12825"
     CDS             <1..865
                     /locus_tag="PKU75_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81806.1"
                     /translation="LSVPGARVGTGSIGGARVRSYAVCFGMPDGAAPELPAGVLADFL
                     TALQTLLYRLTREAADQAGLGRAVRPVELSSGAHVSWSEEGTLLVKVGDPDALEIDPL
                     ADATDAAFAIVLDAITLNTRPPHLTNPVADAVDGLIRVLLDLGRPVAIVLPDGQVATC
                     DPAEIRRDPWQQTRLEPARIGTVTGRLEMVDLRSRRCRIRDPLGQAYDVSDLGNPSEA
                     ARLVGEQVAVSGVVQEGTGTQHHRIEEAHVIPLPARQPAAQQILRPAADQLDLTDDEW
                     RTFLAAHRDDL"
     gene            complement(911..1738)
                     /locus_tag="PKU75_12830"
     CDS             complement(911..1738)
                     /locus_tag="PKU75_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPF81807.1"
                     /translation="MNTALRDGAAIPEIGFGTSPLKGKDAIRAVGSAIEAGYRLIDTA
                     VNYENESEVGEAIRRSGVERAEFVVQSKIPGRDHAYDRAVASVRGSLSRLGLDQLDVG
                     LIHWPNPSVDLYADAWRALVDLQRDGLVRVIGVSNFHPHHLARIVDVTGVVPAINQVE
                     MHPYFPQVALRAEHERLGIITEAWSPLGKGMNAYGEAPVRDAAAAHGVSPAQAILRWH
                     VQLGSVPLPKSENPQRQRENLDVYGFTLTDDELAAITGLARADGRRFGGDPDTHEEM"
     gene            complement(1906..3717)
                     /locus_tag="PKU75_12835"
     CDS             complement(1906..3717)
                     /locus_tag="PKU75_12835"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPF81808.1"
                     /translation="MNARIYRIVVILIGLLAGLGLALPAHAEDGDRIRSFTIDYSIRE
                     DGLVDVVETIDYEFAGSDRHGIYRALITRQPDGDTDQDVLYDIDDIAVSSPDASADFT
                     TSTHSKGLRTKWTELKIGDADETIDDGRAGYRISYTVDGALRTINGIPELYWNATGNG
                     WDAAIDDVRITVNAPAGVTSVSCYQGPAGSTQQCSGAVSDGAAVAQAQGLDKEEGVTI
                     AVQVPAGSVRNAVPTLEPAQTFWGAARLGVGSIAATVGTLVLSLLAFLGMRRANRDRR
                     FGGVTPGIVEPSAPTVIDTIDKDTIPVRFNPPDVPPALGGTLLNDTSASKAAAATLVE
                     LANAGALTIEATPQDAGKFKKSGGMQRVAVARDLGRAPSPYAAKFARDLFKGAPTIVL
                     DKPEKEEAKRFASAVSALNSGVSKIPKEQGWKAKGGFRGSGVLCALAAFFMIFVIVIS
                     FVLLQIGALAYLGPLVLVMCILGAAAAVRLRGYRTPTGRALTDQVEGFKRYLSTAEAG
                     TLRFEEGQDIFSAFLPWAITFGVAERWQKVCRELAEAGRIPDSPVWYSGPAFYSSFDS
                     GSSFGDSLSTSVSSSTSSGSSGGSSGGGGGGGGGGSW"
     gene            complement(3888..5270)
                     /locus_tag="PKU75_12840"
     CDS             complement(3888..5270)
                     /locus_tag="PKU75_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF81809.1"
                     /translation="MTSIEGQPDPAGEALRAAVADGMPRARLDLARLVGLRSVFDPRI
                     EDPAQCAAAASAVADLLTEVGLEVESIETPDGSTAVIGRSTARPAGTAPARPRVLLYT
                     HHDVVPAGDVAAWSSDPWTLTERDGRWYGRGAADCKGNLVAILTALRALSATSAEWPV
                     DLVVLVEGSEEQSAGGMEALARARPDLVAADVIVMADTGNIALGQPTLTTSLRGTGSV
                     RITVRTMEHAAHSGMYGGAAPDALQALLTALASLRSPSGETTIDGLDATGVWEGAAYD
                     PRRFGADAALLDGVKPLGDGQIGDLLWARPAATVLAVDAPPTARVTAAVQGEASAIVN
                     LRVPAVMDPAAAQRLLIAHLRAHTPYGRVEIEPLTLGRGFAARTDGPAYGAMRAAMRE
                     AFGVDPVTTGQGGSIPLTVALSELHPGAEIMLLGVEEPACRIHATDESVEPRELERTA
                     HALALFLAAL"
     gene            complement(5283..6428)
                     /locus_tag="PKU75_12845"
     CDS             complement(5283..6428)
                     /locus_tag="PKU75_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPF81810.1"
                     /translation="MRRKLAINRLRERADLGPELVAQFVERYRSPIIEGQNATFLFLG
                     HADEVFVRHRVVGLPDPLPMKRIGETDLWAATCDLPAGSRVEYQLETRRGDHYERFND
                     PLNERVAHSPMGSSSVCAGEGYHVPSWAVPDPETRPGDLIEHRMRSKALRREQQMLIY
                     TPARFNPVLRYPLLIVHDGPEYVQFSAMKTVLDNLIHRLDIAPMIVAFVPPHDRLREY
                     ANNAPHARFLARELLPMLEEAYPLVESPLGRCLMGASFGGVASVTTGVRYPGVFGSML
                     IQSASLAFTDIGQDHGGGPAFDPVVKFVNRYRAKPTRFTERLYMSCGIYEPLITPNRS
                     MVSVFREVGITVKYEENRDGHNWDNWRDRLQSGLSWLYPGPQKYVYE"
     gene            complement(6425..7363)
                     /locus_tag="PKU75_12850"
     CDS             complement(6425..7363)
                     /locus_tag="PKU75_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81811.1"
                     /translation="MRTILLGPQRFTVRVTGALRSLETEGPVAVINAGWEEREDDIGE
                     LDAALDNRARNLRLFHRLADVLEKDPGFALQASRFRDRHDELTNVYRVRLQHAMGGVY
                     AVQRLVPSHPSRARNSAAYRALADAVAGIRAIDTWYLAEVRRLYGELEVDGGVNSSDV
                     IAWHRAEVRAAQNDCVAWILPGGNIRTLMSALRLFDVTIPHEVSVIAWSAGAMALTER
                     IALFHDHGPEGSQETELFDAGLGRVHGVVAFPHARRRLRMDDPERLGMLALRFEPAEC
                     VLLDDGAVLDFGATGTIPLNARRVGADGLIRAGDDA"
     gene            complement(7375..>7966)
                     /locus_tag="PKU75_12855"
     CDS             complement(7375..>7966)
                     /locus_tag="PKU75_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPF81812.1"
                     /translation="WTTHYYPNVLEAMRTRWISPQFIATNRMSDSDFYDEVAELGHRV
                     IEAFDIGTSATHMEWFYGPKGLRFSEIGCRPPGVGCWDLYTAGNDVDVYREWAHVMTH
                     GTKEREMSRAYSAGLVALRPDRDGVITGYSGIEEVMGRYGQWVIDTHFPHVGHLTQAV
                     EAGYMANAYVRLKHPDYDVLRGMLDDVGRTIHVYGG"
BASE COUNT         1200 a         2819 c         2735 g         1212 t
ORIGIN      
        1 gttgtcggtg ccgggtgcga gagtgggcac cggatcgatt ggaggtgccc gagtgcggtc
       61 gtatgccgtg tgtttcggta tgccggacgg cgcggccccc gagctgcccg ccggggtgct
      121 ggccgacttc ctcaccgcgt tgcagacctt gctctaccgc ctcacccggg aggcagccga
      181 ccaggccggc ctgggtcggg cggtccggcc ggtggagttg agcagcggcg cgcacgtgtc
      241 gtggagtgag gagggcacgc tcctcgtcaa ggtcggcgac cccgatgccc tggagatcga
      301 cccgctcgcc gacgcgaccg acgccgcgtt cgcgatcgtt ctcgacgcga tcaccctcaa
      361 cacccggccc ccgcacctga ccaaccccgt cgccgatgcg gtcgacgggc tcatccgggt
      421 gctgctcgac ctcgggcgcc ccgtcgcgat cgtgctgccc gacggccagg tggcgacctg
      481 cgacccggcc gagatccgtc gcgacccctg gcagcagacg cgcctggagc ccgcccgcat
      541 cggcaccgtc accgggcggt tggagatggt cgacctgcgc tcccggcgct gccgcatccg
      601 cgatcccttg gggcaggcgt atgacgtgag cgacctgggc aatccgtccg aggccgcgcg
      661 tctggtcggc gagcaggtgg ccgtctcggg tgtggtccaa gagggcaccg gcacccagca
      721 ccaccggatc gaggaagcgc acgtcatacc gctgcccgcg cgccagcccg ccgcccaaca
      781 gatcctgcgc ccggccgcgg accagctcga cctgactgat gacgagtggc gcaccttcct
      841 cgccgcgcac cgcgacgatc tctagtccac cacctgcccc gatccggcac agccgcctct
      901 gccggggcgg ctacatctcc tcgtgagtgt cggggtcgcc gccgaaccgg cgcccgtcgg
      961 cgcgggcgag gccggtgatg gcggcgagtt cgtcgtccgt gagcgtgaag ccgtagacgt
     1021 cgaggttctc ccgctgccgc tgcgggttct ccgacttcgg cagcggcacc gagcccagct
     1081 gcacatgcca gcgcaggatc gcctgggcgg gcgaaacccc gtgggccgca gcggcatcgc
     1141 ggacgggcgc ctcgccatag gcgttcatgc ccttgcccag cggtgaccac gcctcggtga
     1201 tgatgccgag ccgctcgtgc tccgcccgta aggcgacctg cgggaagtac gggtgcatct
     1261 cgacctggtt gatcgccggc acgacgccgg tgacgtcgac gatccgggcc aggtggtgag
     1321 ggtggaagtt cgacaccccg atgacgcgga cgagtccgtc gcgttgcagg tcgacgaggg
     1381 cgcgccaggc gtcggcatac agatccaccg acgggttcgg ccagtggatc agccccacgt
     1441 cgagctggtc cagcccgagc cgggacagcg acccgcgcac cgaggcgacg gcacggtcat
     1501 acgcgtgatc ccgccccggg atcttggact gcacgacgaa ctccgcacgc tccaccccgc
     1561 tgcgccggat cgcctctccc acctccgatt cgttctcgta gttgaccgcg gtgtcgatga
     1621 gccgatagcc cgcctcgatg gccgagccga ccgcgcgaat ggcgtccttg cccttcagcg
     1681 gcgaggtgcc gaaaccgatc tccgggatcg ccgcgccatc acgcaaagct gtgttcatgc
     1741 tccaacgcta cggggaggcg gcgtcgatag cggctcggca gctccgggca ttcgatcttg
     1801 accaaccacg tcagccggcc tcgcagagtg tggcgttatc gctgtcctga cgccgataac
     1861 accccccttc aagccaccga tgccgggatc cgccgaaccg gacggtcacc aggacccgcc
     1921 gcccccgccg ccgccaccac cgccggagga gccgccgctg ctgccgctgg acgtggagct
     1981 gctcaccgac gtgctcaggc tgtcgccgaa cgaggacccg gagtcgaagg aggagtagaa
     2041 ggccgggccg gagtaccaca ccggcgagtc cgggatgcgg cccgcctcgg cgagctcgcg
     2101 gcataccttc tgccagcgct cggcgacccc gaacgtgatc gcccacggca ggaaggcact
     2161 gaagatgtcc tgcccctcct cgaagcgcaa ggtgcccgcc tccgcagtcg agaggtaacg
     2221 cttgaacccc tccacctgat cggtcagggc gcggcccgtc ggtgtgcggt agccgcgcag
     2281 gcgcaccgcc gccgcagcgc cgaggatgca catgacgagc accagcggcc ccagataggc
     2341 cagcgcgccg atctgcaaca gcacgaacga gatcacgatg acgaagatca tgaagaacgc
     2401 tgccagggcg cacaggacgc cgcttccgcg gaagccgccc ttggccttcc agccctgctc
     2461 cttggggatc ttcgagacgc ccgagttgag tgcggagacc gcggacgcga agcgcttcgc
     2521 ctcctccttc tcgggcttgt cgagcacgat cgtcggcgcg cccttgaaga ggtcccgggc
     2581 gaacttcgcg gcatacgggg acggcgcgcg gcccaggtca cgcgcgaccg cgacccgctg
     2641 cataccgccg ctcttcttga acttgcccgc gtcctggggc gtcgcctcga tggtcagcgc
     2701 tccggcgttc gccaactcga ccagcgtggc cgcggcggcc ttgctggccg acgtgtcgtt
     2761 gagcaacgtc ccgccgagcg ccggcgggac gtccggcgga ttgaaccgca ccgggatggt
     2821 gtccttgtcg atcgtgtcga tcaccgtcgg cgcgctcggc tcaacgatgc ccggcgtcac
     2881 gccaccgaag cggcggtccc ggttcgcccg ccgcatcccg aggaaggcga gcagcgaaag
     2941 caccagtgtc cccaccgtcg ccgcgatgct gccgacgccc aatcttgctg cgccccagaa
     3001 cgtctgggcc ggctcgagcg tcgggacggc gttgcgcacc gagccggccg gcacctgcac
     3061 cgcgatcgtc acgccctcct ctttgtcgag tccttgcgcc tgcgcgacgg cggcgccgtc
     3121 gctgaccgcg ccggaacact gctgcgtcga gcccgccggg ccctgatagc agctgacgga
     3181 cgtgacgccg gcgggggcgt tgacggtgat gcggacgtcg tcgatcgccg cgtcccagcc
     3241 gttgccggtc gcgttccaat agagctcggg tatgccgttg atcgtgcgca gcgcgccgtc
     3301 gaccgtgtag ctgatgcggt agccggcgcg gccgtcgtcg atcgtctcgt cggcgtcgcc
     3361 gatcttcagc tcggtccact tcgtgcgcag ccccttggaa tgcgtcgacg tcgtgaaatc
     3421 ggccgacgcg tcgggcgagc tgaccgctat gtcgtcgatg tcgtacagga cgtcctgatc
     3481 ggtgtcgccg tcgggttggc gggtgatcag ggcccggtag atcccgtgcc ggtccgagcc
     3541 ggcgaactcg tagtcgatgg tctcgacgac gtcgacgagg ccgtcctcgc ggatcgagta
     3601 gtcgatcgtg aaactgcgga tccggtcgcc gtcctccgcg tgggccggca gcgcgaggcc
     3661 cagcccggcc agcaggccga tcaggatcac cacgatccgg tatatgcgtg cgttcacttc
     3721 tccccctcgt gccggtggtc ccacgcccag gcgtgctcta ctcaaccacc tcatggacgc
     3781 cttgcccagc cgggccgttc cctgagctgc gggcgacgtg ggcgcgtcga gaggggagcg
     3841 tcggcacgct ttcccgcgaa aaccgtaccg attctcccct ctcgacttca gagggcggcc
     3901 aggaagagag cgagagcgtg ggcggtgcgc tccagctccc tcggttcgac gctctcgtcg
     3961 gtggcgtgga tgcggcaggc gggttcttcg acgccgagca acatgatctc ggcgccggga
     4021 tgcagttcgc tgagggcgac cgtgagcggg atcgagccgc cctgtcccgt ggtcaccgga
     4081 tcgaccccga acgcctcccg catcgcggcc cgcatcgcgc cgtatgccgg gccatccgtc
     4141 cgcgccgcga atccccttcc gagcgtgagg ggttcgatct cgacccggcc gtatggggtg
     4201 tgcgcgcgca agtgggcaat caggagtcgt tgtgccgcag cgggatccat gaccgccggg
     4261 acgcggaggt tgacgatcgc gctcgcctcg ccctggacgg cggcggtcac ccgggccgtc
     4321 ggcggggcgt cgacggcgag cacggtggcg gcgggccggg cccacagcag gtcgccgatc
     4381 tgcccgtccc ccaacggttt cactccgtcg aggagtgccg cgtccgcgcc gaagcgccgc
     4441 gggtcgtatg ccgcgccctc ccacaccccg gtcgcgtcca gcccgtcgat ggtcgtctcg
     4501 ccggacggcg aacgcagcga cgccagggcg gtcagcagcg cttgcagcgc gtcgggggcg
     4561 gcgccgccgt acatgcccga gtgcgcggcg tgctccatcg tgcgcacggt gatgcgcacc
     4621 gagccggtgc cgcgcagcga ggtcgtcagg gtcggctgcc cgagggcgat attgcccgtg
     4681 tcagccatga cgatgacgtc ggcggccacc agatcgggac gcgcccgggc cagcgcctcc
     4741 atcccgccgg ccgactgctc ttccgaacct tccaccagca caacgagatc cacgggccac
     4801 tcggcactcg tggcagacaa cgcccgcagc gccgtgagaa tggcgaccag attgcccttg
     4861 cagtcggcgg cgccgcgccc ataccaccgg ccgtcgcgtt cggtgagagt ccacgggtcg
     4921 ctcgaccacg cggcgacgtc ccccgccggc acgacgtcgt ggtgggtgta gagcagcact
     4981 cgcggccgcg ccggagcggt gccggcaggc cgtgccgtcg atcgtccgat gacggcggtg
     5041 gagccgtccg gggtctcgat cgactcgacc tcgaggccga cctcggtgag gagatcggca
     5101 accgcactcg ccgcggccgc gcactgggcc ggatcctcga tgcgcggatc gaagaccgac
     5161 cgcagaccga ccaagcgggc gaggtcgagg cgggcccgcg gcataccgtc ggcgaccgcg
     5221 gcccggagcg cttcacccgc cggatcgggt tgtccctcaa tcgaagtcat cgtcaggccc
     5281 cgctattcgt agacatactt ctgcggcccg ggatagagcc aggacaggcc cgactggagc
     5341 cggtcgcgcc agttgtccca gttgtgaccg tcgcggttct cctcgtattt caccgtgatg
     5401 ccgacctcgc ggaagaccga gaccatggag cgattgggcg tgatcagggg ctcgtagatg
     5461 ccgcacgaca tgtagaggcg ctcggtgaac cgggtcggtt tggcgcggta gcggttgacg
     5521 aacttcacga cggggtcgaa ggccggcccg ccaccgtggt cctgaccgat gtcggtgaag
     5581 gccagcgacg cggactggat gagcatcgag ccgaagaccc cgggatagcg cacccccgtc
     5641 gtgacggagg cgacgccgcc gaaactcgcg cccatcaggc atcgccccag cggcgactcg
     5701 accaggggat aggcctcctc gagcatgggc agcagctccc gcgccaggaa acgggcgtgc
     5761 ggggcattgt tggcgtattc ccgcagccgg tcgtgcggcg gcacgaaggc gacgatcatc
     5821 ggtgcgatgt cgagccggtg gatcaggttg tccagcaccg ttttcatggc cgagaactgc
     5881 acatactcgg gtccgtcgtg gacgatcagc agtggatagc gcagcaccgg gttgaagcga
     5941 gcgggcgtgt agatgagcat ctgctgctca cgccgcaagg ccttgctccg catccggtgc
     6001 tcgatcaggt cgccgggccg ggtctccgga tcgggaacgg cccacgacgg cacgtgatag
     6061 ccctcgccgg cgcacaccga actcgacccc atcggcgagt gcgcgacgcg ttcgttgagc
     6121 gggtcgttga agcgctcgta gtgatcgccg cgtcgcgtct cgagctgata ctccacccgc
     6181 gacccggccg gcaggtcgca cgtcgcggcc cagagatcgg tctcgccgat ccgcttcatc
     6241 ggcagcggat cgggcaggcc gaccacccgg tgccggacga acacctcgtc cgcgtggccg
     6301 aggaagagga aggtcgcgtt ctggccctcg atgatcggcg agcggtacct ctcgacgaac
     6361 tgcgccacca gctcggggcc gagatcggcg cgttcccgga gccggttgat cgcgagtttc
     6421 cttctcacgc atcatcaccc gcccggatca gcccgtccgc gccgacgcgt cgcgcgttga
     6481 gggggatggt cccggtggcc ccgaagtcga gcaccgcccc gtcgtcgagg agcacgcatt
     6541 cggccggttc gaaccgcaac gcgagcatcc ccagccgctc cgggtcgtcc atccgcagcc
     6601 ggcgccgggc gtgcgggaag gcgacgacgc cgtggacccg ccccaggccg gcgtcgaaga
     6661 gctcggtctc ctgcgacccc tccgggccgt ggtcgtggaa gagcgcgatg cgctcggtca
     6721 gcgccatcgc ccccgccgac cacgcgatca ccgacacctc atgcgggatc gtcacgtcga
     6781 agagccgcag cgcactcatc agtgtccgga tgttgccacc cggcaaaatc catgcgacac
     6841 agtcgttctg agcggcacgc acctccgccc gatgccacgc gatgacgtca ctggagttca
     6901 cgccgccgtc gacctcgagt tcgccgtaga gccggcgcac ctccgcgagg taccacgtgt
     6961 cgatggcgcg gatgccggcg acggcgtctg ccagggcgcg gtaggccgcg gagttgcgtg
     7021 cgcgggacgg gtggctcggg acgagccgct gcacggcata cacgcccccc atggcgtgct
     7081 gcaaccggac gcgatagacg ttcgtcagct cgtcgtgccg gtcccgaaac ctactggcct
     7141 gcaatgcaaa cccggggtcc ttctcgagca cgtccgccaa ccggtgaaag agccgcaggt
     7201 tgcgggcgcg gttgtccagg gcggcgtcca actctccgat gtcgtcctcg cgctcctccc
     7261 acccggcgtt gatcaccgcg accggtccct cggtctcgag agagcgcagg gccccggtca
     7321 cgcggacggt gaagcgctgg ggacccagca agatggtgcg cacggcggaa ctggtcagcc
     7381 tccgtagacg tggatggtgc ggccgacgtc gtcgagcatg ccgcgcagca cgtcatagtc
     7441 cggatgcttc aggcgcacat aggcattggc catgtaaccc gcctcgaccg cctgggtcag
     7501 gtgaccgacg tgcgggaagt gggtgtcgat gacccactgc ccgtagcggc ccatgacctc
     7561 ctcgatgccg ctgtatcccg tgatgacgcc gtcgcggtcg ggccgcagcg cgacgagccc
     7621 tgccgagtag gcgcggctca tctcgcgctc tttcgtgcca tgggtcatca cgtgcgccca
     7681 ctcgcgatag acatcgacgt cattgcccgc ggtgtacagg tcccagcagc cgacacccgg
     7741 tgggcggcac ccgatctcgc tgaagcgcaa cccttttggt ccgtagaacc actccatgtg
     7801 cgtggccgaa gtgccgatgt cgaaggcctc gatgacgcgg tggccgagtt cggcgacctc
     7861 gtcgtagaag tccgagtcgg acatccggtt ggtcgcgatg aactgcgggc tgatccagcg
     7921 ggtgcgcatc gcctccagga cgttggggta gtagtgcgtg gtccag
//
LOCUS       DAOPJG010000158         7947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9686_length_7947_cov_6.706031, whole genome shotgun sequence.
ACCESSION   DAOPJG010000158 DAOPJG010000000
VERSION     DAOPJG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9686_length_7947_cov_6.706031"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(417..587)
                     /locus_tag="PKU75_12860"
     CDS             complement(417..587)
                     /locus_tag="PKU75_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81813.1"
                     /translation="MSATPADACPEIRLLTPREVIERADELVGLQRAAYRIEADLIGD
                     DRIPQLSAAAPD"
     gene            682..945
                     /locus_tag="PKU75_12865"
     CDS             682..945
                     /locus_tag="PKU75_12865"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HPF81814.1"
                     /translation="MRRCAARPRHTGLPPPVNTGIEMPEKKLTELGASKRPKVKVTVN
                     GDYSFVTTVASRGGKYFIAFSKARREENGLSAGHPIQVTLSEA"
     gene            1022..2026
                     /locus_tag="PKU75_12870"
     CDS             1022..2026
                     /locus_tag="PKU75_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPF81815.1"
                     /translation="MRYASVDIGKQVSQIGLGTWQFGSKEWGYGPDYDEREAAAIVRR
                     AVELGVTVFDTAEIYGMGRSERILGAALRAAGADVEGLVIATKLFPVLPIAPVVQQRA
                     LASAHRLGVRRLDLYQVHQPNPLVKDGTTMRGMSALIDVGVVGHAGVSNYSLERWQAA
                     DRALAEASPGSGRVVLSNQVQYSLVQRAPERDLIPFAETNGRMVMAYSPLGQGLLSGR
                     FAPGDRVSGVRRANPHFLPDNLTALAPLLDVLRDVASTRQVKPSQVALAWTIRHRCVV
                     AIPGASSVEQLESNVAAAELELTPDEITAIDETAATYRPTTGLAAAPAVAKAMLRSRT
                     "
     gene            complement(1957..2412)
                     /locus_tag="PKU75_12875"
     CDS             complement(1957..2412)
                     /locus_tag="PKU75_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81816.1"
                     /translation="MKLAHAAASLSWTGTWGVGGVGRAVVVDMEGLSGRGAVGVTTMA
                     AAGRRVLSPRARPDSAERRVCRSSFRVGPAGSGLMTLTPDPEFLAAFAPYAEPLAAAT
                     PPPAGEVQGRREGVEGLLAALISGNLCQVRDLSIAFATAGAAARPVVGR"
     gene            2417..2818
                     /locus_tag="PKU75_12880"
     CDS             2417..2818
                     /locus_tag="PKU75_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HPF81817.1"
                     /translation="MYVYVDVLGLYKPGAVRGILDGRVWEFDITQTFAVSALAASLVP
                     TLMIVLSTVMPPRANRPTNLVVAALLIPWMAFNLAGGEWIYYYGLGFAAELVVLALIL
                     RTAWTWPRSSAATGSFTRRVRDAERVPTLAP"
     gene            2815..3963
                     /locus_tag="PKU75_12885"
     CDS             2815..3963
                     /locus_tag="PKU75_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPF81818.1"
                     /translation="MTAVRRSLWDEPRPPHAPPVSRLDRLLVGVLVVVALVEGVARPG
                     LAGQPLVTALAMALMPVLLWRRSRPLLACLIGWGAAGLLSILQIASGSADSGLLSLMV
                     ILVLLYSLVRWGSGREVTVGLVAVALVATLGMYAASASLAEVVGGGLFLLLFVALGAV
                     FRYRASLWHRQEREVRNDERLALARELHDTVAHHISAIAVSAQAGRVLVRGQPERAAA
                     LLASIEDEASRTLAEMRDMVRLLRDDEGGGAAAYAPQRGVADLTALTRPAGTPAVAVR
                     LAGALTGLPRPVDATLYRLAQEGLTNALRHARGATTVVIEVCRVGEEVHLRVTDDGAS
                     APGPRAEPGLGLTGMAERARLLGGSVTAGPGPEGGWVVDAVMPARITP"
     gene            3960..4607
                     /locus_tag="PKU75_12890"
     CDS             3960..4607
                     /locus_tag="PKU75_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF81819.1"
                     /translation="MSIRVVVADDQDLVRAGLVLILGAYPDIEVVGEAADGIAALDLA
                     TRLRPDVLLADIRMPGLDGVEVTRRVAGPDVANPIAVVVITTFDLDEYVLGALRAGAR
                     GFLLKDAGPELLVQAIRAAATGDALIAPNVTRRLLQAFADRSPQAPTQPVEPLTDREE
                     DVLALVARGRTNAEIAAELFIGLSTVKSHIASLMAKLGVRNRVEIAMWAHHTQRV"
     gene            4803..5819
                     /locus_tag="PKU75_12895"
     CDS             4803..5819
                     /locus_tag="PKU75_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPF81820.1"
                     /translation="MLRTHLAAATPSTTLETIATPTLWALTIAGVIALLALDFLLTRK
                     PHEVSMREAVGWSAFYIALPLAFGLWVWTTHGSQTGLEYYTGYLVEKSLSVDNLFIFI
                     LILSSFAVPRVLQQRALLFGIIGALVMRGIFIALGAALIARFSWMFLIFGAILLVTAW
                     GVLRDARHGGHQVDVDELRIVRFARRFYPVTDDYRGTRMSVREAGRRALTPLGLVMLA
                     ILGTDLVFAIDSVPAVYGITGDPYLVFATNAFALLGLRALYFVLEGALGKLRHLGYGL
                     AAILAFIGVKLVLHWAHGVWTWVPTIPTVASLAVIVAALSVTTVTSVLANRREASVLP
                     SGHR"
     gene            complement(5895..6929)
                     /locus_tag="PKU75_12900"
     CDS             complement(5895..6929)
                     /locus_tag="PKU75_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPF81821.1"
                     /translation="MPERPAGTVEDRAQEQGPGFVMRAVTRPEYGGPEVVTVEDVARP
                     SPGPGEVLVRVGAAGLDRATLHLLTGLPLMARLAFGVRRPKRPVLGQQVAGVVEEVGP
                     GVDGLAVGDRVCGTAAGSFAQYAVAKAGTLAPTPDGVEDRHAATIGVSGVTALAAVVE
                     HARVQRGDRVLVLGASGAVGGYAVQLAAHLGADVTGVCSASKEDLVRAQGAHRTVDYA
                     SVRLPAMGGPFDVIIDIAGNNPVSALRRCLAPGGRLVIVGGEGGGRLLGGIERNLLAT
                     MAGWFTAKDLGWMFSSTTTARCAEVARLITEGAVTPVIDRTVDLDGAADAIAAMASGH
                     LRGHAVLEMQ"
     gene            7014..7661
                     /locus_tag="PKU75_12905"
     CDS             7014..7661
                     /locus_tag="PKU75_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF81822.1"
                     /translation="MPAKKLTPDAVLVTALAIADRHGIEALTMRGLARELGVEAMSLY
                     HHVASKDVLLDVMVDRVYAEIALPAPDGDWREELRRRSASVREVLRRHPWALPLMESR
                     RSPGPANLAYHEANIACLRAAGLTPAQVAHAYAVVDAFVYGFVLQELMLPFDSGPEAA
                     AMIRDEPWGAAVTAHPNMAWFAEHVVLAPGYTFEREFAPGLALVLDGIAESFGLP"
     gene            complement(7689..7841)
                     /locus_tag="PKU75_12910"
     CDS             complement(7689..7841)
                     /locus_tag="PKU75_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81823.1"
                     /translation="MTKRFLGHGVNSTDPWILGLSGPAAFQPNLAGYEVYAAALTAAI
                     SPRSLR"
BASE COUNT         1079 a         2985 c         2707 g         1176 t
ORIGIN      
        1 aagcggccag cccctgtgca atctgatgtg gattcacgcc taccggagga gatgccttgg
       61 tatgccgggt cgctgcggct gattcgcgag tacctggagg gcgacgggac gatcgaggac
      121 gtcctcgctc tcgacgcggg cggcggccac gttgtctcgg cgatgtggca tgggatctgg
      181 agcgggcaga tcccccgtac agccgtcgcg ccggccggtt ggctcggccg gtcgcgacga
      241 cggcaggacc gcgggcagcg cgctccacga gggcgcggcc gagaccgcgt cgcaggtgct
      301 cggggtccac gaccaggcgc tcgatgtcgg ttgtcccgtc ggccagccga ctgaatgcca
      361 gggcgcgacc acctgctcgt cagccaatgc caccaaccac ccgagtccgg ccgcgatcag
      421 tcaggtgccg ccgcgctgag ttgcgggatg cggtcgtcac cgatgagatc ggcctcgatc
      481 cggtatgccg ctcgctgcag tcccaccagc tcatcggcgc gttcgatgac ctctctcggc
      541 gtcagcagcc ggatctccgg gcacgcgtca gcaggagtgg cgctcaccgc cacagtgcac
      601 cacatccagc ccatcgtgcc gacgtccggt gtgtgaagcg agagcgcgcg gacgcgcagg
      661 ggaaccggcc gaccactcga ggtgaggcgc tgcgcagccc gtccgcggca taccggtctg
      721 ccgcccccgg tcaacaccgg gatcgagatg cccgagaaga agctgaccga gctcggcgcg
      781 agcaagcggc cgaaggtgaa ggtcacggtg aacggcgact actccttcgt gaccacggtg
      841 gccagccgcg gcgggaagta cttcatcgcc ttctcgaagg ctcggcgcga ggagaacggc
      901 ctcagcgcgg gccacccgat ccaggtcacc ctgagcgagg cctgatcccg gcagtcggcc
      961 cacggcgccg ggcaccccgg cggaggttgc cgaggcggca gccactgtgt gggatgggtg
     1021 catgaggtac gcgagcgtcg acatcggcaa gcaggtctcc cagatcgggc tggggacgtg
     1081 gcagttcggc tccaaggagt ggggctacgg tcccgactac gacgagcgcg aggcggcggc
     1141 gatcgtgcgg cgcgccgtcg agctcggcgt caccgtcttc gacaccgcgg agatctacgg
     1201 gatgggccgc agcgagcgca tcctcggcgc ggcactgagg gcggcgggcg cggacgtcga
     1261 gggcctcgtc atcgcgacca agctcttccc ggtgctgccc attgcacccg tcgtccagca
     1321 gcgggccttg gccagcgccc accggctcgg cgtgcgccgg ctcgacctct accaggtgca
     1381 ccagccgaac ccgctcgtca aggacggcac gacgatgcgc gggatgagcg cgctcatcga
     1441 cgtcggggtc gtcgggcacg cgggggtcag caactactcg ttggaacgat ggcaggctgc
     1501 cgatcgcgct ctcgcagagg cctccccggg cagcggccgg gtcgtgctga gcaaccaggt
     1561 gcagtacagc ctcgtgcagc gcgcgccgga gcgggacctc atcccgttcg ccgaaaccaa
     1621 cggccggatg gtcatggcct actcgccgct ggggcagggg ctcctctcgg gccggttcgc
     1681 gcccggggac agggtcagcg gggtgcgtcg ggccaacccg cacttcctcc cggacaacct
     1741 gacggcgctc gccccgctcc tcgacgtcct gcgggacgtc gcgtcgaccc gccaggtcaa
     1801 gccctcccag gtggcgctgg cctggaccat ccgccaccgc tgcgtcgtcg ccatccccgg
     1861 ggcctcgagc gtcgagcagc tcgagagcaa cgtcgccgcg gctgagctcg agctgactcc
     1921 agacgagatc accgcgatcg acgagaccgc ggccacctac cgaccgacca ccggcctggc
     1981 cgctgcaccg gccgtcgcga aggcgatgct caggtcgcga acctgacata gattgccgga
     2041 gatgagcgcg gcgagcagtc cctcgacgcc ctcgcgacgg ccctggacct cgccggcggg
     2101 gggtggggtg gcggcggcca ggggctcggc atacggcgcg aacgcggcca ggaactcggg
     2161 gtcaggggtg agcgtcatga gcccgctgcc cgccggaccg acccggaagg acgagcggca
     2221 tacccgcctt tcggccgagt caggtcgggc ccgcgggctg aggactcgac ggccggccgc
     2281 ggccatcgtg gtgacaccaa ccgctcctcg acccgaaagg ccctccatgt caaccacgac
     2341 agcccgcccc acgccgccca ctccccacgt cccggtccag ctcaggctcg ccgcggcgtg
     2401 ggccagcttc atggtcatgt acgtctacgt cgacgtcctc ggcctctaca agcccggcgc
     2461 cgtccggggc atcctcgacg gccgggtatg ggagttcgac atcacccaga ccttcgccgt
     2521 cagcgccctc gccgcctccc tcgtgccgac gctgatgatc gtgctctcca cggtcatgcc
     2581 accccgggcc aaccgcccca ccaacctcgt cgtcgccgcc ctcctcatcc cgtggatggc
     2641 gttcaacctc gcgggcgggg agtggatcta ctactacggc ctcggcttcg ctgccgagct
     2701 cgtcgtgctc gccctcatcc tccggacggc ctggacgtgg ccgcgatcga gcgccgcgac
     2761 cgggtctttc acacgtcgag tgcgcgacgc ggagcgcgtc cctacgctgg cgccgtgacg
     2821 gctgtgcggc gctccctctg ggacgagccg cgcccacccc acgcaccgcc ggtcagccgg
     2881 ctcgaccggc tgctcgtcgg tgtgctggtg gtggtcgcgc tcgtcgaggg cgtcgcccgc
     2941 ccgggcctgg ccgggcagcc cctcgtcacc gccctcgcga tggcgctcat gcccgtcctc
     3001 ctctggcgcc gcagccgccc actcctggcc tgcctcatcg gctggggcgc cgccggcctg
     3061 ctctcgatcc tccagatcgc ttctggcagt gcagattccg gactgctgtc cctgatggtc
     3121 atcctcgtcc tgctctactc cctcgtccgt tgggggtccg gtcgcgaggt caccgtgggg
     3181 ctggtcgccg ttgccctggt cgccaccctc ggcatgtatg ccgcgtcggc gagcctcgcc
     3241 gaggtcgtcg gcgggggcct cttcctgctg ctcttcgtcg cgctcggcgc ggttttccgc
     3301 taccgcgcca gcctctggca ccgccaggag cgcgaggtcc gcaacgacga gcggctggcc
     3361 ctggctcgcg agctccatga caccgtggca caccacatct cggccatcgc ggtctccgcc
     3421 caggccggcc gggtgctcgt gagggggcag cccgagcgcg ctgccgcgct cctcgcctcg
     3481 atcgaggacg aggcgtcccg cacgctcgcc gagatgcgtg acatggtgcg gctgctccgc
     3541 gacgacgagg ggggaggcgc cgcggcatac gcccctcaac gaggcgtcgc cgatctgacg
     3601 gcgctcaccc ggcccgccgg cacgcccgcg gtcgcggtcc ggctggcggg cgccctcacc
     3661 ggcctgcccc gacccgtcga cgccacgctc tatcgcctcg cccaggaggg cctgaccaac
     3721 gccctccgcc atgcgagagg tgcgacgacg gtggtcatcg aggtatgccg cgtgggcgag
     3781 gaggtccacc tccgcgtcac cgatgacggc gcctccgctc ccggtccgcg cgccgagccc
     3841 ggcctcggcc tgaccggcat ggcagagcgt gcgcggctcc tcggcggatc cgtcacggcc
     3901 ggccccgggc ccgagggcgg ctgggtcgtc gacgcggtca tgcctgcgag gattacgccg
     3961 tgagcatccg cgtcgtcgtc gccgatgacc aggacctggt gcgggccggg ctcgtcctca
     4021 tcctcggggc ctacccggac atcgaggtcg tgggggaggc ggccgacggg atcgcggccc
     4081 tcgacctcgc gacccgactg cgacccgatg tcctcctcgc cgacattcgc atgccgggac
     4141 tcgacggcgt ggaggtcact cgtcgggtgg ccgggccgga cgtggcgaac cccatcgccg
     4201 tcgtcgtcat cacgaccttc gacctcgacg agtacgtcct gggcgccctg cgtgccgggg
     4261 cgcgcgggtt cctgctcaag gacgccggcc ccgagctgct cgtccaggcc attcgcgcgg
     4321 ccgcgaccgg ggacgccctc atcgcgccga acgtgacccg ccgcctgctc caggccttcg
     4381 ccgaccggtc acctcaggcg ccgacccagc cagtcgagcc gctcaccgac cgcgaggagg
     4441 acgtgctggc gctggtggcc cggggccgca cgaacgccga gatcgccgcg gagctcttca
     4501 tcggactgag cacggtcaag tcgcacatcg cctcgctcat ggccaagctc ggcgtccgca
     4561 accgggtcga gatcgcgatg tgggcccacc acacccagcg ggtctgagga cggccgcccc
     4621 ctcgtcgagg tggcgggccg cgcagttgcc ggggtgcgca cccgtcacgt atcctcggtc
     4681 gccacgaggg gagtacttcc caagtcggcg ccggtcattt cgcgcggccc atgccgcgcc
     4741 cggcgcgcgc cccacccggg gtgaaggaga cctcgcaccg ttagatcacg atgcgaggac
     4801 tcatgctccg cacccacctg gccgctgcca ccccctcgac cacgctcgag accatcgcca
     4861 cccccaccct gtgggcgctg acgatcgccg gcgtcatcgc cctactcgcc ctggacttcc
     4921 tcctcacccg caagccgcac gaggtgtcga tgcgagaggc cgtcgggtgg tccgccttct
     4981 acatcgcgct cccgctcgcc ttcgggctgt gggtatggac cacgcacggc tcccagaccg
     5041 ggctcgagta ctacaccggc tacctcgtcg agaagtccct gtccgtcgac aacctcttca
     5101 tcttcatcct catcctgtcc tcgttcgccg tgccgagggt gctgcagcag cgggccctgc
     5161 tcttcggcat catcggcgcc ctcgtgatgc gggggatctt catcgccctc ggggccgccc
     5221 tcatcgcccg cttctcctgg atgttcctca tcttcggcgc gatcctgctc gtcaccgcgt
     5281 ggggcgtcct gcgcgacgcc cgccacggcg ggcaccaggt cgacgtcgac gagctgcgca
     5341 tcgtccggtt cgcccgccgg ttctacccgg tcaccgatga ctatcgcggc acccgaatga
     5401 gcgtccgtga ggccggccgc cgcgccctga cccccctggg gctggtcatg ctcgcgatcc
     5461 tcgggacgga cctcgtcttc gccatcgact cggtgcccgc cgtctacggc atcaccggcg
     5521 acccctactt ggtcttcgcc acgaacgcct tcgcgctgct cggcctgcgc gccctctact
     5581 tcgtcctcga gggcgcgctc ggcaagctcc gccacctcgg ctacggcctc gccgcgatcc
     5641 tcgccttcat cggggtcaag ctcgtgctcc actgggcgca cggcgtgtgg acctgggtgc
     5701 cgaccatccc gaccgtcgcc tccttggccg tgatcgtcgc cgccctctcg gtgaccacgg
     5761 tgacgagcgt gctcgccaac cggcgggagg cctcggtgtt gccctccggc caccgctgag
     5821 cacgccgctc gggagggctg gccgagcggc ggtatgccag gcggccaccc agccgtccgg
     5881 cataccgaag gctgtcactg catctcgagc acggcgtgac cgcgaaggtg acctgacgcc
     5941 atggcggcga tcgcgtccgc cgcgccgtcc agatcgaccg tgcggtcgat gacgggcgtg
     6001 accgccccct cggtgatgag gcgggcgacc tcggcgcagc gcgcggtcgt cgtgctcgag
     6061 aacatccatc cgaggtcctt ggcggtgaac cagccggcca tggtcgcaag caggttgcgc
     6121 tcgatcccgc cgaggagccg cccacctcct tctccgccga cgatgacgag gcggccgccc
     6181 ggcgccaagc agcgccgcag ggcggagacc gggttgttgc cggcgatgtc gatgatgacg
     6241 tcgaacggtc cacccatggc agggaggcgc acggacgcgt agtcgaccgt ccgatgcgcc
     6301 ccctgcgcgc ggaccaggtc ctccttgctc gcgctgcaca ccccggtgac gtccgccccg
     6361 aggtgtgccg ccagctgcac ggcataccct ccgaccgcgc ccgacgcccc gaggacgagg
     6421 acccggtctc cgcgctggac acgagcgtgc tcgacgaccg ccgcgagggc ggtgacgccc
     6481 gagaccccga tcgtcgccgc gtgacggtcc tcgacgccgt ccggggtcgg ggcgagggtg
     6541 ccggctttgg cgaccgcata ctgggcgaac gacccggcgg ccgtcccgca cacacgatcc
     6601 ccgacagcga gcccgtcgac gccggggccg acctcctcca cgacgccggc tacctgctgg
     6661 cccaggaccg gccgcttcgg ccgccgcacc ccgaaggcca ggcgcgccat gaggggcagg
     6721 ccggtgagga ggtgcaaggt cgcgcggtcg agcccggcgg caccgacccg gacgagcacc
     6781 tcaccggggc cggggctcgg ccgggcgacg tcctcgacgg tgaccacctc ggggccgccg
     6841 tactcggggc gggtcaccgc ccgcatgacg aacccgggcc cctgctcctg ggctcgatcc
     6901 tcgactgtcc ccgctggccg ttcgggcatc tcgactccta tacatacgtc gtaaggcttg
     6961 aacaccatac attacgacgt aaggtttggc gcaagacctc gggaggatct gcgatgccag
     7021 caaagaagct caccccggac gccgtcctcg tgaccgccct cgccatcgcc gaccgccatg
     7081 gcatcgaggc gctgaccatg cgcggcctgg cccgcgagct cggggtggag gcgatgtcgc
     7141 tctatcacca cgtcgcgagc aaggacgtcc tgctcgacgt gatggttgac cgggtctatg
     7201 ccgagatcgc gctgcccgcg ccggacgggg actggcgcga ggagctgcgc cggcgctcgg
     7261 cgtcggtgcg cgaggtgctg cgccgccacc cgtgggcgct gccgctcatg gagtcgcgac
     7321 gctcacccgg gccggccaac ctcgcctacc acgaagcaaa catcgcctgc ctgcgcgccg
     7381 ccggcctgac gccggcccag gtcgcccatg cgtatgccgt cgtggacgcc ttcgtctacg
     7441 ggttcgtcct ccaggagctc atgctcccgt tcgacagcgg tcccgaggcc gcggccatga
     7501 tccgcgacga gccatggggc gcggcggtga ctgctcaccc caacatggcg tggttcgccg
     7561 agcacgtcgt gctcgcgccg ggctatacct tcgagcgcga gttcgcgccc ggcctggcgc
     7621 tcgtcctgga cgggatcgcg gagtccttcg gcctgccctg accgtgaggg ggccgggcga
     7681 ggccgacctc agcgcaggct gcggggactg atcgcggcgg tgagcgccgc ggcataaacc
     7741 tcgtaaccgg cgaggttcgg ctggaaggcc gccggtcccg aaaggccgag gatccacggg
     7801 tcggtcgagt tgaccccgtg cccgaggaag cgcttggtca cgtcgacgaa ctggaagccg
     7861 tgccgtgccg cggcggccgc gatgacagct tttctggctc atcacaatcc agggtgtagg
     7921 cgggacctga agccaccggt ttcgagc
//
LOCUS       DAOPJG010000159         7874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9803_length_7874_cov_7.891802, whole genome shotgun sequence.
ACCESSION   DAOPJG010000159 DAOPJG010000000
VERSION     DAOPJG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9803_length_7874_cov_7.891802"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..323
                     /locus_tag="PKU75_12915"
     CDS             111..323
                     /locus_tag="PKU75_12915"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HPF81824.1"
                     /translation="MKTTLYLPEDIKGAVEVEARRRGVSEAEVIREALRTALLSQPVA
                     PRGGLFRGQEPIADRVDEVLAEGFGT"
     gene            320..727
                     /locus_tag="PKU75_12920"
     CDS             320..727
                     /locus_tag="PKU75_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPF81825.1"
                     /translation="MIVDTSALLAYFDAGEPQHDAVAAIIEGSRDPLIVSPFVIAELD
                     YLVLTRHGSDAEQAVLSELSGGAWELALMDRERIRAATRLVETYSDIPIGITDASNLV
                     LAQAYQTRDIATLDRRHFQILRFSDGSAPRILP"
     gene            complement(791..1642)
                     /locus_tag="PKU75_12925"
     CDS             complement(791..1642)
                     /locus_tag="PKU75_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HPF81826.1"
                     /translation="MPAAADLCCAKIVHRDNLTPGMVRLVLGGEGLRGWTSNGFSDAY
                     FVVWLPRPGATYAAPFDVERIRAEHPEEWWPVHRHYSVRRWDAASGELTVDFVVHGDE
                     GVAGPWARKAQVGDQVMVGRPGGAYIPDPGADWHLMVGDESALPAIGAALEQLPAGAE
                     AVLIALCDGPEFEVHLGSLATVEAHWLHRCGEPTDARLLVDAVRALPWRVGRVHAFVH
                     GEAGEVRKVRRHLLGERGMPRADLSVSGYWRRTMTDEAWRRVKREWNADVERDLPADT
                     DLAARPG"
     gene            complement(1651..2478)
                     /locus_tag="PKU75_12930"
     CDS             complement(1651..2478)
                     /locus_tag="PKU75_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81827.1"
                     /translation="MTAEHTLVAANVTLGYGDRTVVEDLTWAVSPSRITVIVGANACG
                     KSTVLRSLARLLTPTEGRVLLDGKVLHDQPTKEVARQLGLLPQSPTAPEGIVVGDLVA
                     RGRSPHKRMLSRWSASDDAAVAEALELTDTDDLVDRPVDELSGGQRQRVWIAMALAQR
                     TDILLLDEPTTYLDVSHQIEVLDLLTDLNRERGTTIVMVLHELNLAARYADHLVAMNR
                     GRLVAQGPPEHVLTVETVREVFGMESAVIPDPVSGKPLVLPVGRHHAARHPRVPAFT"
     gene            complement(2475..3485)
                     /locus_tag="PKU75_12935"
     CDS             complement(2475..3485)
                     /locus_tag="PKU75_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPF81828.1"
                     /translation="MRAGRRRRSRRYAVAVAVLTLILLVAFLASLIVGQRHYPLGDVL
                     RVIGGQDVPGASYTVGRLRLPRAALAVLAGAAFGLGGVSFQTMLRNPLASPDIIGITS
                     GASAAAVYALVILHLDPSAASVIAIVAALGTALAIYGLAARGGLHGTKLILIGIGVSA
                     MLHSVVSYVIIRAAQWDLQTAMRWLNGSVNGATWSRVLPLAMAMVAIIPILLSRTRAL
                     TLLEQGDDTATALGVRTGRTRLLTIVGTVVLLAFATAAAGPIAFVAFLSGPIASRLVG
                     TGRSLMVPAMLVGASLTLLADFAGQFAFGARYPVGVITGVLGAPYLVYLLIRTNRTGG
                     SL"
     gene            complement(3545..4522)
                     /locus_tag="PKU75_12940"
     CDS             complement(3545..4522)
                     /locus_tag="PKU75_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPF81829.1"
                     /translation="MGWLLISLVVLLVLVALSVTLGARHVAVSDVVAALGGDTGTVSR
                     AAVSTRIPRTVLALVAGAALGVSGAVMQGLTRNPLADPGMLGITPGASLAVVGCIAFV
                     GMTSPIATVWVAIAGAAVTAVIVYLLGSMGRGGATPLKLSLAGAAVTASISSLISAIL
                     LPRADVMNSFRFWQIGGVGGADFARIGMVAPFLAAGFFLSIAVTRGLNALALGDDVAA
                     GLGEHVGLLRSLAGIGAVLLCGAATAVAGPIAFVGLVVPHSCRLLTGGDHRWLVPFSA
                     VAGGGLLVVADIVGRVAGRPAEIDAGIVTAIIGAPVFIAIVRRQKGRAP"
     gene            complement(4594..5691)
                     /locus_tag="PKU75_12945"
     CDS             complement(4594..5691)
                     /locus_tag="PKU75_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HPF81830.1"
                     /translation="MFPSRPLTRVATLMLVGVLAACGASSENTASTGAAEATSGGAAT
                     APSTTTSDSSSAKSEAAAFPVTIKHAFGETTIAAKPERVATVAWANHEVPLALGVVPA
                     GMAKATWGDDDGDGILPWVEDKLTELGGETPALFDETDGIDFEGVANTNPDVILASYS
                     GLSQDDYDQLSEIAPVVAYPETAWTTSMDEMITMNAEAIGMKGEGEQLVADLTTQVTD
                     SAAEHPALQGKTALFAWVDPKDMSKIGYYTTGDPRAALLEQVGLDTAQVVKDNADATN
                     FFQEISAEEVDKLADVDIIVTYGDDTGELLKTLQGNDLLATIPAVKNGAVVSLVDNTP
                     LAAAANPSPLDIPWGLNDYLDLLDAAAAKAP"
     gene            5810..6640
                     /locus_tag="PKU75_12950"
     CDS             5810..6640
                     /locus_tag="PKU75_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HPF81831.1"
                     /translation="MTRRSAADHRRKPEHSRAIRLTVRSRAAVTPHMSRVTLGGNGLH
                     AWEHMGFDQWFRLFLAPAGASLQHVPDSLTKASYARFMLAPASRRPLIRNYTVRAFRP
                     SGPDGPELDVDLVAHGCADEGTAGPASIWARECAVGDEVAIINEGTMFAAPEGATVRL
                     AADESGLPAAAGILRDLPRGTAGLAVIEVPTEEDRQPLDAPERVVVHWVVRQTPASRP
                     GAAALARLRSLPPPTAPSYAWVAGESDLATGARRHWVSQGIDKGDISFCGYWKAGAAH
                     "
     gene            6652..7683
                     /locus_tag="PKU75_12955"
     CDS             6652..7683
                     /locus_tag="PKU75_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPF81832.1"
                     /translation="MTRLETLDALESAVGKRPATAMLKSISQLDGHCLDVLSLSTFGV
                     LGVLSEARRLSSHTIGGPAGVFHTLEPGRLQLPGLPHAGYGRPAGLVAFVAGWGETLR
                     VNGRLDADTLIVEEAFLHCAKCVLRSRLWSPLVEHEATIERSGTTQSSVLRSGLADPV
                     VSGLLTRAPFATIATADGAGRADISPKGDPSGLVRLLGNGEIGIPDRPGNMRRDTFHN
                     ILERPEVSVLALMPGEETVVEVTGTAVVDDDPDLRAIFEVRGNTPVAVMRVRVESALA
                     QRSSAIGAARLWDPKQHVPQGSLPRASRIWTDHVNATIASAKEAPIPALHEPDLAAGL
                     QADYVQNLY"
BASE COUNT         1291 a         2697 c         2736 g         1150 t
ORIGIN      
        1 gtgcgcgtga tcgacccggc cgaggacacg gccgccggct cgtcgcccgg ctgatgccaa
       61 ccctgacgcc gaagcaccgg ctcaatgcca taacaggtac catatatggc atgaagacga
      121 ccctgtatct cccggaggac atcaagggcg ccgtcgaagt ggaggcccgg cggcgcgggg
      181 tgtccgaggc cgaggtgatc cgtgaggcat tgcggaccgc gctgctgagc caaccggtgg
      241 cgccccgggg cgggctcttc cggggccagg aaccgatagc ggatcgcgtc gatgaggtgc
      301 tcgccgaggg cttcggcacg tgattgtcga taccagcgca ctgctcgcgt acttcgatgc
      361 cggtgaacct cagcatgacg cggtcgccgc catcatcgag ggcagcaggg acccgcttat
      421 cgtgtcaccc ttcgtcatcg ccgaactgga ctatctcgtc ctcacccggc acggatcgga
      481 cgccgagcag gccgtgctgt cggagttgtc gggcggtgcg tgggagctcg ccctcatgga
      541 ccgggagcgg atccgggccg ccacccgtct cgtcgagacc tactccgaca tcccgatcgg
      601 catcaccgac gcgtcgaatc tggtgttggc tcaggcgtat cagacccgcg acatcgccac
      661 cttggaccga aggcacttcc agatcttgcg cttctccgat ggctcggcac cacgcatcct
      721 gccctgagca ggagcagcca cgagatgacg ccggcccggt tcgaccctcg ccgaccgggg
      781 tgggtcggac tcatccgggc ctggcggcga gatcggtgtc ggcggggaga tcccgctcga
      841 cgtcggcgtt ccattcgcgc ttgacccggc gccacgcctc gtcggtcatc gtgcggcgcc
      901 agtagccgga gaccgagagg tcggcccgcg gcataccgcg ctcgccgagc aggtgccgcc
      961 ggactttacg cacctcgccg gcttccccgt ggacgaacgc atgcacccgg ccgacgcgcc
     1021 acggcaacgc gcgcaccgca tcgacgagca atcgcgcgtc ggtgggttcg ccgcagcggt
     1081 gcagccagtg cgcctccacg gtcgccaggc tgccgaggtg gacctcgaac tcgggcccgt
     1141 cacagagggc gatgaggacc gcctcggcac ctgcggggag ctgctcgagg gccgcaccga
     1201 tggcgggcag ggcggattcg tcgccgacca tcaggtgcca gtccgcgccc gggtccggga
     1261 tgtacgcccc gcccggccgt cccaccatga cctggtcgcc gacctgggcc ttgcgtgccc
     1321 agggaccggc caccccctcg tcgccatgga cgacgaagtc gaccgtcagc tcgccggagg
     1381 ccgcgtccca cctgcgcacc gagtagtgcc ggtggaccgg ccaccactcc tcaggatgct
     1441 cggcccggat cctctcgacg tcgaaagggg cggcatacgt cgcaccggga cggggcagcc
     1501 agacaacgaa gtacgcgtcg ctgaacccat tactcgtcca accacgtaga ccctccccgc
     1561 cgagcacgag acgaaccatc cccggcgtga ggttgtcgcg atggacgatc ttggcgcagc
     1621 agaggtcggc ggcagcaggc atcgggactc ctatgtgaag gctgggacac ggggatggcg
     1681 ggcagcgtgg tgccggccca cgggcagcac gagtggcttg ccggagaccg gatccgggat
     1741 gaccgccgac tccattccga agacctcgcg caccgtctcg acagtcagga cgtgctccgg
     1801 cgggccctgt gcgacgagcc ggccgcggtt catggcgacg aggtgatcgg cataccgcgc
     1861 agcgagattc agctcgtgca gcaccatgac gatcgtcgtg ccgcgctcgc ggttgaggtc
     1921 ggtgagcagg tcgagtacct cgatctggtg gctgacgtcg agataggtcg tcggttcgtc
     1981 gagcagcagt atgtcggtgc gctgagccag ggccatcgcg atccagaccc gctggcgctg
     2041 cccgccggag agctcgtcga cgggccggtc gacgaggtcg tcggtgtccg tcagctccag
     2101 agcctcggct accgccgcgt cgtcggaggc ggaccacctg ctcagcatgc gcttatgcgg
     2161 gctgcgcccc cgggcgacca ggtccccgac gacgatgccc tcgggagccg tgggcgactg
     2221 gggaagcaaa cccagttgtc gcgcaacctc tttggtcggc tggtcgtgca gcaccttccc
     2281 atcgagcagc acccggccct cggtcggggt cagcagccgg gccagcgagc gcagcacggt
     2341 ggacttgccg cacgcgttcg cgccgacgat gacggtgatc cgactcggcg acacagccca
     2401 ggtgaggtcc tcgacaacgg tccgatcgcc atacccaaga gtgacattcg cggcgacgag
     2461 cgtgtgttcg gccgtcatag ggagcctccg gtgcggttgg tccgaatcaa gaggtagacc
     2521 aggtagggcg cgccgagcac accggtgatg acaccgacgg gatatcgcgc gccgaatgca
     2581 aactgtccag cgaagtcggc cagcagcgtg agtgacgcac cgacgagcat tgccggcacc
     2641 atcagcgacc ggccggtccc gacgaggcgg gaggcgatcg gtcccgacag gaaggcgacg
     2701 aaggcgatcg gacccgcggc ggccgtcgcg aaggcgagca ggacaacagt gcccacgatg
     2761 gtgagcaggc gcgtgcggcc agtgcggacg ccgagggctg tggcggtgtc gtcgccctgc
     2821 tcgagcagcg tcagcgctcg ggtcctggac agaaggatgg gaatgatcgc caccatcgcc
     2881 atcgcgagcg gcagcacgcg cgaccaggtg gccccgttga cactgccgtt gagccagcgc
     2941 atcgccgtct gcagatccca ttgcgccgca cggatgatca cataggaaac caccgagtgc
     3001 agcatcgccg acacgccgat cccgatgagg atcagcttcg tgccgtgcag cccgcctcgc
     3061 gccgcgaggc cgtagatcgc cagcgcggtc ccgagcgcgg caacgatggc gatgacggag
     3121 gcagccgagg gatccaggtg caggatgacg agggcgtaga ccgctgccgc gctcgccccg
     3181 gaagtgatgc cgatgatgtc cggtgacgcc aacgggttgc gcagcatcgt ctggaagctc
     3241 accccgccca ggccgaaggc cgctccggcg aggacggcca gcgccgcccg cggcaaccgc
     3301 agccggccga ccgtgtagga ggcgcccggt acgtcctgcc cgccgatcac ccgcagcacg
     3361 tcaccgaggg ggtaatggcg ttgtcctact atcagactcg cgaggaaggc gacgagaagg
     3421 atcagggtca aaacggctac ggcaacggca taccggcgac tgcgccgccg gcgacctgcg
     3481 cgcagggcgg cgatcgtcgc accacgatct actgaccgga cgggcgcgac ggacgtggag
     3541 gcagtcacgg cgcacgtccc ttctgccgcc gcacgatggc gatgaagacg ggggccccga
     3601 taatggcggt gacgatgccc gcgtcgatct ccgcgggccg gccggcgacc cgtccgacga
     3661 tgtcggcgac caccagcaag ccgccaccgg caaccgcgga gaagggcacg agccaacggt
     3721 gatcgccgcc ggtcaacagc cggcaggagt gtggcacgac gagtccgacg aaggcgatcg
     3781 gcccggccac cgcggtcgct gctccgcaca gcaggacggc cccgatgccg gcgagcgacc
     3841 ggaggaggcc gacgtgctca ccgagcccgg ccgccacgtc gtctccgagc gcgagggcat
     3901 tcagcccgcg tgtcacggca atggagagga agaatccggc ggccaagaag ggcgcgacca
     3961 tcccgatccg cgcgaaatcc gccccgccga cgccaccgat ctgccagaac cggaaggagt
     4021 tcatcacgtc cgcacgcggc aggaggatgg cgctgatcag cgagctgatg gacgcggtga
     4081 cggcggcacc ggcgagagag agcttcagcg gcgtcgcgcc gccgcgtccc atcgacccga
     4141 ggaggtagac gatcacggcg gtgacggctg ctccggcaat tgcgacccag acagtggcga
     4201 tgggcgaggt catccccacg aaggcgatgc acccgaccac ggccagcgag gcgccggggg
     4261 tgatgccgag catcccgggg tcggcaagcg ggttgcgggt caggccctgc atcaccgcac
     4321 ccgagacacc cagggcggcg ccggccacaa gcgccaggac ggtgcgggga atgcgcgtgg
     4381 aaactgctgc gcgcgagacc gttccggtgt ccccacccaa ggcggccacg acatcgctca
     4441 ccgcgacatg acgtgccccc aaagtcacgg acaaggcaac caggaccagc aggacgacga
     4501 gggagatcag cagccatccc aggcgcgaac cggctgcggg ccgctgcgcg gcgacccgcg
     4561 acgccggcgg gcccggcgcg cagagtgcct gtctcacggg gccttcgcgg cggccgcgtc
     4621 taggaggtcg aggtagtcgt tcagccccca ggggatatcg agcggcgacg ggttggcggc
     4681 ggcggccagc ggggtgttgt cgacgagcga gacgacggcg ccgttcttga ccgccgggat
     4741 cgtggcgagc aggtcgttgc cctgaagcgt cttgagaagc tcgcccgtgt cgtcgccata
     4801 ggtgacgatg atgtcgacgt cggcgagctt gtcgacctct tcggcgctga tttcctggaa
     4861 gaagttcgtc gcatcggcgt tgtccttgac gacctgcgcg gtgtcgaggc ccacctgctc
     4921 gagcagggca gcgcgcgggt cacccgtcgt gtaatagccg atcttgctca tgtccttggg
     4981 atcgacccag gcgaagaggg ccgtcttgcc ttgcagcgca gggtgttccg cggcggaatc
     5041 ggtgacctgg gtcgtcaggt cggcgacgag ctgttcgccc tcgcccttca tgccgatcgc
     5101 ctcggcgttc atggtgatca tctcgtccat ggacgtcgtc caggccgtct cgggataggc
     5161 gacgaccggc gcgatctcgc tgagctggtc gtaatcgtct tgggagagac cggaatacga
     5221 cgccaagata acgtcggggt ttgtgttcgc gacgccttcg aagtcgatgc cgtcggtctc
     5281 gtcgaagagc gccggggtct cgcccccgag ttcggtgagt ttgtcctcga cccacggcag
     5341 tatgccgtcg ccatcgtcgt cgccccacgt ggccttggcc atcccggccg gaacgacgcc
     5401 gagggccaaa ggcacctcat ggttggccca ggcgaccgtc gcgacgcgct cgggctttgc
     5461 cgcaatggtg gtctcgccga aggcgtgctt gatggtgacc gggaaggctg ccgcctcgga
     5521 cttcgccgac gacgagtccg atgtcgtggt cgagggtgcg gtagccgcac ccccgctggt
     5581 ggcttccgcg gcaccagtgg aggcggtgtt ttcggacgag gcgccacacg cggccagcac
     5641 cccaacgagc atcagcgtgg ccacacgagt cagcgggcgg gacgggaaca cgaacgatct
     5701 cctccagtag attaattagg taaggctaac ctaagctgag atgacggtcg gcgccaatgt
     5761 ggcccagcgc cggagtcccc gacacccgcg atatcccgaa tggagcacca tgacccgccg
     5821 cagcgccgcg gatcaccgcc gcaagcccga gcactcccga gcgatccggc tgaccgttcg
     5881 gtcacgtgcc gctgtcactc cgcacatgtc ccgggtcaca ttgggcggca acggacttca
     5941 cgcctgggag cacatgggtt tcgaccagtg gttccggctg ttcctggctc cagccggggc
     6001 gtccctccaa cacgtcccgg attcgctgac gaaggcgagt tatgcccgct tcatgctcgc
     6061 gcccgcgagc cggcggccgc tcatccgcaa ctacaccgtc cgcgcgttcc gaccctccgg
     6121 gccggacggc ccggaactcg atgtcgacct cgttgcccac ggctgcgccg acgagggcac
     6181 cgcaggcccc gcgtcgatct gggcccgaga gtgtgcggtc ggtgacgagg tggcgatcat
     6241 caacgagggc accatgttcg ccgcgccgga gggggcgacg gtcaggcttg cggccgacga
     6301 gtccggcctg ccggccgccg cgggaatcct gcgtgacctg ccgcgcggga cggccggttt
     6361 ggccgtgatc gaggtgccga ccgaggagga ccggcagccg ctggacgctc ccgagcgggt
     6421 ggtggttcat tgggtcgttc ggcagacacc ggccagccgg cccggtgccg ctgcgctcgc
     6481 ccggctgcgc tcccttccgc caccgacagc gccgagctac gcctgggtcg ccggcgaatc
     6541 ggacctggcc accggagctc gccgccactg ggtctcccag ggcatcgaca agggggacat
     6601 cagcttctgt ggctattgga aggcgggtgc ggcgcactag cgtcgattcg tatgacgcgc
     6661 ctggagaccc tcgacgccct cgagtccgcg gtcgggaagc gacctgccac cgcgatgctg
     6721 aaatcgatct cgcagctgga tgggcactgc ctggatgtgt tgtcgctgag caccttcggt
     6781 gtccttggcg tcctctccga agcgcgccgg ctctcgtccc acaccatcgg gggacccgcc
     6841 ggggtcttcc acaccctcga gccgggccgg ctgcagttgc ccggcctgcc gcatgccggg
     6901 tatggccgtc ctgccgggct cgtcgcgttc gtcgccggct ggggcgagac cctgcgcgtc
     6961 aacggccggc tcgacgctga caccctcatc gtcgaggaag ccttcctgca ctgcgccaag
     7021 tgcgttctcc ggtcgcgcct gtggtccccg ctggtcgagc acgaggcgac gattgaacga
     7081 agcggcacaa cacaatccag cgtactgagg tcggggcttg ccgaccccgt cgtgtccggt
     7141 ctcctcaccc gcgcgccctt cgccacgatc gccacggcgg acggcgccgg ccgcgcagac
     7201 atcagcccca agggcgaccc gagcggtctg gtgcgcttgc tcgggaacgg agagatcggc
     7261 ataccggacc gtccgggcaa tatgcgccgc gacaccttcc acaacatcct ggagcgcccc
     7321 gaggtctccg tcctggccct gatgcctggc gaggagacag tcgtcgaggt gacgggaacc
     7381 gccgtcgtcg atgacgatcc ggacctgcgg gcgatattcg aggtgcgcgg caacactccg
     7441 gtcgccgtca tgcgcgttcg ggtcgaatcg gctctcgccc agcggagttc ggcgatcggt
     7501 gccgcgcggc tgtgggatcc gaagcagcac gtgccccagg gatcgttgcc ccgcgcgagc
     7561 cggatctgga ccgaccacgt caacgccacg atcgcgtccg cgaaggaggc acccattccc
     7621 gccctgcacg agcccgacct ggccgcgggg ttgcaggcgg actacgtcca gaacctctac
     7681 tgacggcgct cgcacgtggc agcggcgctc cggtccgtct atggcagcat gactcgacat
     7741 gccggagatt cccttcccac gcaccggtgc cccggcgtta cgcgccctgg agggggccgg
     7801 atactcccat ttgcaggagc tggacggcat accgatgcgg tcggtgttgg cgttgcctgg
     7861 catggacccc aagg
//
LOCUS       DAOPJG010000160         7848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9867_length_7848_cov_6.964840, whole genome shotgun sequence.
ACCESSION   DAOPJG010000160 DAOPJG010000000
VERSION     DAOPJG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9867_length_7848_cov_6.964840"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1022)
                     /locus_tag="PKU75_12960"
     CDS             complement(135..1022)
                     /locus_tag="PKU75_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPF81833.1"
                     /translation="MTTPTFSLNNGVTIPVIGLGVFQTPPEETVAAVRAALETGYRMI
                     DTAAAYGNEREVGEALAQSGIEREDVFLETKVWVTDYGRDETLHAFEKAIGKLGVERL
                     DLLILHQPDIGNFEKTVAAYGALEELLADGRVRAIGISNFTPALLDRLLAETEVVPAI
                     NQIELHPYFTQVASREANAAHGILTQAWSPIGGISAYRGTSAATPTFTNPTLVEIGER
                     HGKTTAQVMLRWHIQRGTQVIPKSTRPQRIAENFDVFDFDLEQDELAMIDGLDTGARG
                     GPDPEQPQPSFHSFIIPEA"
     gene            1115..2101
                     /locus_tag="PKU75_12965"
     CDS             1115..2101
                     /locus_tag="PKU75_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPF81834.1"
                     /translation="MGVVDWVDRLQRRHPVFGFPLAVIYKFFDDAGGYLAALLTYYAF
                     LSFFPVLLLLSTVLSILLRNHPEWQEDVLNSALSQFPVIGDQLGDPRSLSGGAAGVAI
                     GSALAIYGALGAGNALQYTMNTVWAVPRNSRPNPFLARLRSGMLILTAGLAAIATTVV
                     STFAAATTGWGVWTKVATTLLTLVINTGIVMLCFRIGVRGTARRHQVAGAIITTLAWQ
                     LLQTFGVVFVGRVVKGASATNGIFALVIGLLAFIYLISVVVVLSAEVNVVHARRLYPR
                     ALLTPFTDNVDLTEADKQAYAAQARAMRSKGYERIHVEFGRRGDPPDDDLDD"
     gene            complement(2108..2521)
                     /locus_tag="PKU75_12970"
     CDS             complement(2108..2521)
                     /locus_tag="PKU75_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPF81835.1"
                     /translation="MEKRGIPTTTRGPADWFTGDVHVENFATGADASRLVAAHVRFTP
                     GARTNWHSHGLGQTLHCTSGTGFVVTRDGVVQVLRPGETVWTPPGEEHWHGATPDTML
                     CHDALLVAPETGDATTWLEPVTDEQYAAAHRALDS"
     gene            complement(2652..3074)
                     /locus_tag="PKU75_12975"
     CDS             complement(2652..3074)
                     /locus_tag="PKU75_12975"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81836.1"
                     /translation="MGGNARAKALYAPVYAEPSPNLSRHVFLDPAARTFSPDWERMAD
                     INVGTLRLALARYPDDADLIALVDELAAANADFRERWPQHPVMQFGSGTKVVDHLTVR
                     RMALQHEQLRLVADVTLVVNVYSAAPGSIDAQRFAALG"
     gene            3264..>3691
                     /locus_tag="PKU75_12980"
     CDS             3264..>3691
                     /locus_tag="PKU75_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81837.1"
                     /translation="MRTTSDAPRTFPRSRRTFRRLPFGYAAMSRRGIPDDLLHDWFGP
                     ARRDKDVRRDFAKFATGAPDRATLLSWFERMRRFDRPVLVVWAAQDTMMPAAHGPRLA
                     ALYPNSRLEVVEDSATLIPEDQPERLAALIRDFALAPHRHT"
     assembly_gap    3692..3791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3805..4482)
                     /locus_tag="PKU75_12985"
     CDS             complement(3805..4482)
                     /locus_tag="PKU75_12985"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPF81838.1"
                     /translation="MPFARRPPTAQAQVRSGVGELTDAFARLHTGSLRERQRVEMMRR
                     VQLTAMDLFEDKGFDKVTIEDVAAAAEVSPSTIYRHFLTKEGLVLHDEHDDDLLAVVP
                     GLLAEHNLYDAFDIALRAFGDEHFMAGDDLARRRTRLWFQVPSIRAAAGLMADDVADS
                     LARLVADSPHHALSLPQARVVLGAFVGAVFTAIRVWEEGGATEDLRGLLLDTIGLLRD
                     PSATHRS"
     gene            4536..5447
                     /locus_tag="PKU75_12990"
     CDS             4536..5447
                     /locus_tag="PKU75_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81839.1"
                     /translation="MRESLSSSVVTARGIRKNFGAVTALDGLDLDVRAGEVHGFLGPN
                     GAGKSTTIRAILGQLRLTGGQLRVFGRDPMDTVAVHERLAYVPGDTALWPGLSGGECI
                     DLIGQFNGHLDAGRRAELIERFELDPTRKARTYSKGNRQKVALIAALATDVEFYLLDE
                     PTSGLDPLMENRFQETIREVKADGRTVLLSSHILDEVEALCDRVTIIRSGRTVSTGSL
                     EDLRAHTLTTITVTTARPIQGLEAMAGVGAVVGEKCAAGVRTQLSTTPDALPDVLTRL
                     AAERPTAVHVRPPSLDELFLEQYQQAS"
     gene            5444..7015
                     /locus_tag="PKU75_12995"
     CDS             5444..7015
                     /locus_tag="PKU75_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81840.1"
                     /translation="MTGTLRILVLHLRTARRMLLAWALGLSLLVVLVARSLTALYPDL
                     GDRIAYATSSSSSQAAIALNGRWGDLTTLGGITTNEVGFLGFLVFPLAGVLIAVGLTR
                     RQEDFGRVELLTSGRLGRLAPLAGALLAGVLAILAFGVIATAGLIAVDLPAAGALRYA
                     ALLTVFALSWLAIGAVCAEVSQDGRTATGFALLLILAAYVARTVVDAAGWDLGWWTPM
                     GWLPLAAPFGAWRWQPYAALTLLTILAATVAGVVARHRDLGSGLIVPRPGPAFARTGL
                     GTPLGYAWRMTRGITLAWLVGLGVWSVALGALSADMTSIVRANPQLLEVLGIDMLEDF
                     VTSLALVIGALGAAALGVQGIARLSQEEAAGRLGLVLATPVERRRLIGVWGALIAVGS
                     AVVILAQGLLTGLATWASTGSRDALGRSLWAALVLTVPVLLVVAFALALRGLHPAWDA
                     ATWVVVGWGAVVGFLGTALDLPAWARDLSPIEAVGRVPMADPRPWPLGILLLGGLALA
                     VLGVHAAGRRDLAKG"
     gene            7039..7464
                     /locus_tag="PKU75_13000"
     CDS             7039..7464
                     /locus_tag="PKU75_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HPF81841.1"
                     /translation="MSDDALWDLLARPGLRGVLTTLKRDGRPQLSVVFHHYDAASKTL
                     SISVTDTRAKTKNLRRDPRASYLVLGDTPWQYVVAEGIAEVGDVARAPDDAAADALVE
                     LYRAQAGEHDDWDEYRAAMVADQRLVLRIRVERVYGMIA"
BASE COUNT         1112 a         2653 c         2675 g         1308 t
ORIGIN      
        1 ggaacgacgc gacccgctga cgcctttcgc cagataggta cgacggtttg ggtgtcataa
       61 ccgccagagc gtcttaccta cctgccggta acgccaggag tgcaacgacc atcggcgtcg
      121 atccgaggta gtggtcacgc ctccggaatg atgaaggagt ggaagctggg ctgcggctgc
      181 tcggggtcgg gccccccacg tgcgccggtg tcgaggccgt cgatcatcgc gagttcgtcc
      241 tgttccaggt cgaagtcgaa gacgtcgaag ttctccgcaa tgcgttgcgg ccgtgttgat
      301 ttcggaatga cttgcgtccc gcgctggatg tgccagcgca acatcacctg agcggtggtc
      361 ttgccgtggc gctccccgat ctcgacgaga gtcgggttgg tgaacgtcgg cgtcgcagcg
      421 ctggtccccc ggtaggcaga gatcccgccg atgggggacc acgcctgggt gagtatgccg
      481 tgggccgcgt tcgcctcccg gctcgccacc tgggtgaagt aggggtgcag ttcgatctgg
      541 ttgatcgccg gaacgacctc ggtctcggcg agcagccggt cgaggagggc gggggtgaag
      601 ttgctgatgc cgatggcccg cacccggcca tcggcgagca attcctccaa cgcgccgtag
      661 gcggccaccg tcttctcgaa gttgccgatg tcgggttggt gaaggatgag cagatcgagc
      721 cgttcgaccc cgagtttgcc gatggccttc tcgaaggcgt gcagggtctc gtcccggccg
      781 tagtcggtga cccagacctt ggtctcgagg aagacgtcct cgcgctcgat tccgctttgc
      841 gccaaggcct cgccgacctc gcgctcgttg ccgtaggccg cagccgtgtc gatcatccgg
      901 tagcccgtct cgagcgccgc cctgacggcg gcgacggtct cctccggtgg ggtctggaag
      961 acgcccagcc cgatcaccgg gatcgtgacg ccgttgttga gggagaaggt gggggtggtc
     1021 atgatggtcc tctcaggagt cggtgcggca ttgcggaccg ctcgtgtgcg acgcggagtc
     1081 aagactcgaa ccaccctacg ggcatgattg cgccatgggt gtcgtggact gggtggaccg
     1141 cctgcagcgc cggcacccgg tcttcggttt cccgctcgcg gtgatctaca agttcttcga
     1201 cgacgccggc ggctacctgg cggccctgct gacgtactac gcgttcctgt cgttcttccc
     1261 cgtgttgctg ctgctgtcga ccgtgctgtc gatcctgctg cgcaaccacc ccgaatggca
     1321 ggaggacgtc ctcaactccg cgctgagcca gttccccgtg atcggcgacc agctcgggga
     1381 cccgcgctcg ctctccggtg gtgcggccgg tgtggccatc gggtcggcgc tggcgatcta
     1441 cggtgccctg ggcgccggga atgcgttgca gtacaccatg aacacggtct gggcggtccc
     1501 gcgcaacagc cgtccgaatc cgttcctcgc gcggctgagg tcggggatgc tcatcctcac
     1561 ggctggactc gcggccatcg ccacgacagt cgtgtcgacg ttcgccgccg cgacgacggg
     1621 ttggggtgtg tggaccaagg tcgccacgac tttgctcacg ctcgtcatca atacgggcat
     1681 cgtgatgctc tgcttccgca tcggcgtgcg cggcacggcg cgtcgtcatc aggtggcggg
     1741 ggcgatcatc acgacactcg cgtggcagtt gttgcagacg ttcggtgtcg tcttcgtcgg
     1801 ccgggtggtg aaaggcgctt ccgcgacgaa cggcatcttc gccctggtca tcggtctgct
     1861 ggccttcatc tatctgatct ccgtcgtcgt cgtgctcagc gccgaggtca acgtcgtcca
     1921 cgcacgccgg ctctatccgc gggccctgct caccccgttc accgacaatg tcgacctcac
     1981 cgaggccgac aagcaggcgt acgccgcgca ggcgcgggcc atgcgcagca agggctacga
     2041 acgcatccac gtcgagttcg ggcgccgggg cgacccgccg gacgacgacc tcgacgactg
     2101 acacggctca gctgtccaac gcgcggtggg cggcggcata ctgctcgtcg gtcaccggct
     2161 ccaaccacgt tgtggcatcc cccgtttcgg gagcgacgag caaggcgtcg tggcacagca
     2221 tcgtgtccgg agttgcgccg tgccagtgct cctcgccggg aggggtccac acggtctcgc
     2281 cgggtcgcag cacctggacg acgccgtcgc gggtgacgac gaagccggtc cccgacgtgc
     2341 agtgcagggt ctggcccagc ccgtggctgt gccagttggt ccgggcgccc ggcgtgaacc
     2401 ggacgtgggc ggcgacgagg cgcgaggcgt cggcgccggt ggcgaagttc tcgacgtgga
     2461 catcgccggt gaaccagtcg gccgggccgc gggtggtggt gggtatgccg cgtttctcca
     2521 tggccatgag tgaggctcct tcgggtgggg tcagggtcgc tgcagcggta ggccgctcac
     2581 tcaccactgt gactggcgct gcaccttccg ctcaaccccc gtggggtggg ccagtggcac
     2641 cccccgacct gtcagcccag cgcggcgaac cgttgcgcgt cgatgctgcc cggcgccgcg
     2701 ctgtagacgt tgacgaccag cgtgacatcg gccacgagcc gcagctgttc gtgctgcaat
     2761 gccattcgtc tgaccgtgag atgatcgaca acctttgtgc ccgagccgaa ttgcatcaca
     2821 gggtgttgcg gccaccgctc ccggaagtcg gcatttgccg ctgccaattc gtcgaccagc
     2881 gcgatgaggt cggcatcgtc cggatagcgg gcaagggcca ggcgaagcgt gcccacgttg
     2941 atgtccgcca tgcgctccca gtccggtgag aaggtccgag cggccggatc gaggaagacg
     3001 tgcctcgaca gattgggcga cggttcggca tacaccggcg cgtacagggc cttggcgcgg
     3061 gcgttgccgc cgatgacatc atccggtgat tgcgaacgag gaccgtcacc tccgccctgg
     3121 cgtcggtcat ctgctgcagg ccgggcggca tgtcagcgcg ggcaccatgc cgtggcgggc
     3181 gaccggacgc gttgacctgg cgcaggaggt cgagaaagta gccgcgttcg atcgcgctga
     3241 gccgcagcgc gtccatgagc gcggtgagga cgacatcgga tgccccgcgc acatttccgc
     3301 gctccaggcg caccttccgc aggctgccct tcgggtatgc cgcgatgagc cgccgcggca
     3361 tcccggacga cctgctccac gactggttcg gtcccgcccg ccgggacaag gacgtgcgcc
     3421 gggacttcgc gaagttcgcg acgggcgcgc ccgaccgggc caccctgctg tcgtggttcg
     3481 agcggatgcg gcgcttcgac aggccggtcc tggtcgtctg ggccgcccag gacaccatga
     3541 tgcccgccgc ccacggcccc cgtctcgctg cgctctatcc caactcccgc ctcgaggtcg
     3601 tcgaggacag cgccaccctg atccccgagg accaacccga gcggctggcc gccctcatcc
     3661 gcgactttgc actggccccg catcgacaca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nacggggtgg ggcgtcagga tcggtgcgtc gcggagggat cgcgcaacag
     3841 gccgatggtg tcgagtagca accctcgcag gtcctccgtc gcgccgcctt cctcccacac
     3901 gcggatagcg gtgaacaccg cgccaacgaa agcgccgagg acgacccggg cctgcggcag
     3961 tgacagagcg tggtgggggg agtccgcgac cagccgggcc aaggagtcgg cgacgtcgtc
     4021 ggccatcagc ccagcggcgg cgcggatgga cggcacttgg aaccagagcc gcgttcgccg
     4081 tcgcgccagg tcgtcaccgg ccatgaagtg ctcgtcgccg aaggcccgca gcgcgatgtc
     4141 gaaggcgtcg tagaggttgt gctcggccag cagcccgggc acgacggcga ggagatcgtc
     4201 gtcgtgctcg tcgtgcagca cgagcccttc cttggtgagg aagtgccggt agatcgtgct
     4261 cggagacacc tcggcggcgg ctgcgacgtc ctcgatcgtg accttgtcaa aacccttgtc
     4321 ctcgaacaga tccatcgccg tcagctgcac gcgtcgcatc atctccacgc gctggcgttc
     4381 gcgcagcgag ccggtatgca gtcgcgcgaa cgcgtctgtc agctcgccga cgccggagcg
     4441 cacctgtgcc tgcgcggtcg gggggcggcg ggcgaacggc atacggccac tctaattgtt
     4501 gacagtcact ttcacgagaa cataaagtga ctctcatgag agagtcactg tcaagttcag
     4561 tggtgacggc cagggggatt cgcaagaact tcggtgccgt gacggccctc gacggcctcg
     4621 acctggacgt gcgcgccggc gaggtccacg gcttcctcgg tcccaatggg gccggcaagt
     4681 ccacgacgat ccgggccatc ctcggccaac tgcggttgac cggcggtcag ctgcgcgtct
     4741 tcggccggga cccgatggat accgtcgcgg tgcacgaacg gctcgcctac gtacccggcg
     4801 acacggccct gtggccgggc ctgagcggcg gggagtgcat cgacctcatc gggcagttca
     4861 acgggcacct cgacgccggc cgccgggccg agctcatcga gcgcttcgaa ctcgacccca
     4921 cccgcaaggc ccgcacctac tccaagggca accggcagaa ggtcgccctc atcgcggcgc
     4981 tcgccaccga cgtcgagttc tatctcctcg acgagccgac ctccgggctc gatccgttga
     5041 tggagaaccg ttttcaggag acgatccggg aagtgaaggc ggacggccgc actgtcctgc
     5101 tcagcagcca catccttgac gaggtcgagg ccctctgcga ccgcgtgacg atcatccgca
     5161 gcggccgcac cgtatccacg ggcagcctgg aggatctgcg cgcgcacacc ctcacgacga
     5221 tcacggtcac gactgcccgc ccgatacagg ggctggaggc gatggcgggg gtgggcgcgg
     5281 tggtcgggga aaagtgtgcc gcgggggtgc gcacgcagtt gtccacgacg cccgatgccc
     5341 tcccagatgt cctgacccgc cttgccgccg agcgccccac agccgtccac gtgcggccgc
     5401 cctcgctgga cgaactcttc ctcgagcaat accagcaggc atcgtgaccg gcaccctgcg
     5461 cattctggtg ctgcacctgc gcacggcccg ccgcatgctg ctcgcctggg cactcgggtt
     5521 gagcctgctc gtcgtcctgg tcgcccgcag cctcacagct ctctaccccg acctcggcga
     5581 ccgaatcgcc tacgccacaa gcagttccag ctcacaagct gccattgccc tcaatggcag
     5641 atggggcgac ctgaccactc tgggcggcat cacgaccaac gaggtcgggt tcctgggctt
     5701 cctcgtcttc cccctcgccg gcgtcctcat cgccgtcggg ttgacccgtc gccaggagga
     5761 cttcggccgc gtcgagctgc tcacgtcggg ccggctcggc cggctcgctc cgctcgccgg
     5821 tgccctgctc gccggtgtcc tggcgatcct cgctttcggt gtcatcgcga ccgccggcct
     5881 gatcgccgtc gatctgcccg ccgccggggc gctgcggtat gccgcgctcc tcaccgtctt
     5941 tgcgctctcg tggctcgcta tcggggcggt ctgcgccgag gtgagccaag acggccgcac
     6001 cgcaacgggt ttcgcgctgc tcctcatcct cgccgcgtat gtcgcccgaa cggtcgtcga
     6061 cgccgccggc tgggatctgg gttggtggac gccgatgggc tggttgccct tggcggcgcc
     6121 gttcggcgcg tggcgctggc agccgtatgc cgccctcacc ctcctgacga tcctcgccgc
     6181 cacggtcgcc ggtgtcgtcg cccggcatcg cgacctgggt tctggtctca tcgtcccgcg
     6241 tcccggaccg gcttttgcga gaacgggtct cggcaccccc ttggggtatg cgtggcggat
     6301 gacgcggggc atcacgctcg cctggctggt cgggctcggc gtgtggtcgg tggctctcgg
     6361 cgcgctcagc gcagacatga cctctatcgt ccgggccaat ccgcaactgc tggaggtgct
     6421 cggtatcgac atgctggagg attttgtgac cagcctcgcg ctcgtcatcg gcgctctcgg
     6481 cgcggccgcg ctcggcgtgc aaggaatagc ccggctgtca caggaggagg cagccgggcg
     6541 cctcgggctc gtcctcgcca cgccggtcga gcggcgccgg ctgatcgggg tctggggggc
     6601 gctgatcgcc gtcggctcag cggtcgtcat tctcgcccag ggactcctca ccggcctggc
     6661 gacgtgggcc agcacgggat cacgcgacgc tctggggcgg tcgctgtggg ccgcgctcgt
     6721 cctgacggtc ccggtgctgc tcgtcgtggc ttttgcgttg gcgctcaggg gacttcaccc
     6781 agcgtgggac gccgcaacgt gggtcgtcgt cggatggggc gccgtcgtcg gcttcctcgg
     6841 caccgcgctc gacctgcccg cctgggcccg cgatctctcc cccatcgagg ccgtcggccg
     6901 cgtgccgatg gcggacccgc ggccgtggcc actcggcata ctcctcctcg gcggcctggc
     6961 cctggcggtg ctcggcgtcc acgcggcggg ccggcgcgac ctggcgaaag ggtgagcggt
     7021 ccgcatacgg tggaacgcat gagcgatgac gccctctggg acctcctcgc gcggcctggg
     7081 ctgcgcgggg tcctgacgac cctcaagcgc gacggccggc cgcaactgtc ggtcgtcttc
     7141 caccactacg acgcggccag caagaccctc tcgatctccg tcaccgacac ccgagccaag
     7201 accaagaacc tgcgccgcga cccgcgcgcg agctatctgg tgctcggcga cacgccctgg
     7261 cagtatgtcg tcgccgaagg catcgcggag gtgggagacg tggcgcgggc gccggatgac
     7321 gccgcggccg acgcgctcgt cgagctctat cgtgcgcagg cgggagagca cgacgactgg
     7381 gacgaatacc gcgcggcgat ggtcgcggac cagcggctcg tcctgcggat ccgggtcgag
     7441 cgggtctacg ggatgatcgc ctaaaggcgc gaccagcacc gccagcgggc aggatggacg
     7501 gtatgagcga tgccgaggag tcccggctgc ccgagtgggc gcagctgatc gatcagggtg
     7561 ggccgctcta gcggtatgtc gcagcgacgc cgatggggga gttcaccgtc cacacccacg
     7621 gcgggcaggt gacgagctgg gtcggccacg gtctcgagcg gctctggacc tcggaacggc
     7681 cgagtatgcc gcgggcaagg ccatccgtgg cggcatcccg gtgatcttcc cctggttcgc
     7741 gaacggccat gggggagaat gcaaaccctc ccacgggctc gcccgcctgg ccacgtggga
     7801 gctcgcggga tggggcacga ccgacgagcc cgacgacacg atggacct
//
LOCUS       DAOPJG010000161         7835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9890_length_7835_cov_7.192674, whole genome shotgun sequence.
ACCESSION   DAOPJG010000161 DAOPJG010000000
VERSION     DAOPJG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9890_length_7835_cov_7.192674"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKU75_13005"
     CDS             complement(<1..401)
                     /locus_tag="PKU75_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPF81842.1"
                     /translation="MPSPADRLARLTRKPERALGTREEMNRLLDGQLVGTLATVVDGQ
                     PWVVPMLYGRDGDRILLHGSTGAGALRQVASGAPAAFCVTAVDGLVVAESTFESSANY
                     RSVVIRGSLESVTGQTKSDVLQRITARIIAGR"
     gene            complement(438..1739)
                     /locus_tag="PKU75_13010"
     CDS             complement(438..1739)
                     /locus_tag="PKU75_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81843.1"
                     /translation="MTWRRRLAGLRVDLTPARESRDYRLLLIAGTVFYLGHMVSYVAL
                     PFLIYRETGSNAMVGLMGVVELVPLIVFGLWGGALADHVDRRRLLVTCGIAQVVLMAA
                     LAANAFRQRPSIVVIFVIGALLISVGAVQRPSRESLLPRTVRHDQISQANALSSFGMQ
                     AGLFVGPTIAGLLIATVGIGWCFVVDFAGMLFATAMYARMRPYPHRGETTAPSVRGIA
                     EGWTYAVRRRDLLGTYLIDIAAMLLAMPIVLFPAFATDVFERPELVGLLYSAETVGAL
                     VATSLSGWMHRVHHHGRAITIAATAYGCAIALVGLAPTIWLAMLALALAGAADNVSAV
                     FRGTVWNQTIPERMRGRLAGIEMLSFSVGPLGGQARAGLVADAWSVRGAITSGGLACV
                     AGVALTAAALRDFWRYDARTDPHAVAERSARAAAGESVTDS"
     gene            complement(1736..2830)
                     /gene="mnmA"
                     /locus_tag="PKU75_13015"
     CDS             complement(1736..2830)
                     /gene="mnmA"
                     /locus_tag="PKU75_13015"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPF81844.1"
                     /translation="MRVVAAMSGGVDSAVAAARMLDAGHEVVGVHLALSRSAATLRES
                     ARGCCTIEDAGDARRVADRLGIPFYVWDLADRFQRDVVDDFVAEYAAGRTPNPCLRCN
                     EKIKFSALLDKALALGFDAVATGHYAQIVDGPDGRQLHRAVDAAKDQSYVLGVLTPDQ
                     LDRAFFPLGDTTKPRVREEAAERGFFIAKKPDSYDICFIPDGDTRGWLTDRLGEQPGD
                     ILDADGQVVGHHHGAYGFTIGQRKGLGLERSSMDGQARYVTAIDRAANTVTIGTADLL
                     GVDEIRGIRALWCGPAPSGPIAVGAQVRAHGEEIPATAHVEGDEIVVRLGRTIRGVAP
                     GQSVVLYDGTRVVGSATIAEAGRARHTTGA"
     gene            complement(2830..4008)
                     /locus_tag="PKU75_13020"
     CDS             complement(2830..4008)
                     /locus_tag="PKU75_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPF81845.1"
                     /translation="MHYLDHAATTPLVPAARAALIETLELVGNPSAVNAAGRAARRIV
                     EEARERIAAALGAHPSEVIFTSGGTEADNLAVKGTYAARHTAEPARTRVVTSAIEHHA
                     ILDSVDYMTESSGATAVFVEPTVVGAIAPDALTSALGDGADVALVSIMWGNNEIGTIQ
                     PIAQLAAVCRELGIPMHSDAVQAVGHVPVDFSASGLDLLTLTGHKLGGPVGSGALLAR
                     RDAALAPQLHGGGQERKVRSGTLDARGVHALAAAVETAVAGLDDEMTRVIALRDKLIR
                     GALALGLGIEVTGAWTPGSAADRLPGNAHLRVPGADRDALLFVLDAAGISCSAGSACT
                     AGVTRPSHVLTALRIGDADAAGALRFSLGHTSTEEDVDAVLAVLPDAVARSQAARGAA
                     "
     gene            complement(4199..5155)
                     /locus_tag="PKU75_13025"
     CDS             complement(4199..5155)
                     /locus_tag="PKU75_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPF81846.1"
                     /translation="MAEIFVLVDHANGQVRKTTAEMLTIAARLGEPSAVYLGPGAAEA
                     AATLGKFGAGRVYAMDSADLTGYLVVPQAEFLAALVGKLKPGAVLIPSNPAGKEIAAR
                     VAVKTGSGLITDAVDVQPGDGGIETTKSVFAGSYTVKAKVTKGTPIICVKPNSAAPQE
                     KPAAGQVVPVNLTISDAAKTAQITEAKEKQASGRPELTEAAIVVSGGRGTGGDFSPVE
                     TFADSLGAAVGASRAAVDAGWYPHTSQVGQTGKQVSPQLYVACGISGAIQHRAGMQTS
                     KTIVAINKDPEAPIFELVDFGVVGDLFTVLPQATEKVQASKG"
     gene            complement(5187..5969)
                     /locus_tag="PKU75_13030"
     CDS             complement(5187..5969)
                     /locus_tag="PKU75_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPF81847.1"
                     /translation="MNIVVCVKYVPDAQADRGFDEADLTTNRESVDGLLSELDEYAVE
                     EALKIAESGEGAVTVITIGPDQAADAVKKALQMGADKGVHVNDEAIKGSDALATSLIL
                     AEAIKKVGMPDLVLTGMASTDGTMGVVPAMLAERLALPQVTYASELTVDGGTVTARRD
                     GDTASETIVGAVPALISVTDQINEPRYPSFKGIMAAKKKPVETWTLADLGIDAGQVGA
                     AGSSTEVISVAKRPPREQGEIVTDEGDGGTKLAEFLAGRKFI"
     gene            complement(6165..6944)
                     /locus_tag="PKU75_13035"
     CDS             complement(6165..6944)
                     /locus_tag="PKU75_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPF81848.1"
                     /translation="MGEFVTLEVEDGIGTIRLTRPPMNALNLQVQSELGEAAAAASAD
                     PAVAAVIVYGGEKVFAAGADIKQMQQMSYADMATAAGGLQGFTRALAAVPKPTVAAIT
                     GYALGGGCEVALACDFRIAAQDAKLGQPEILLGIIPGAGGTQRLARLVGPARAKEIVY
                     SGRFVDAAEALAIGLVDKVVPADQVYAAARDWMSRFVGGPALALRAAKEAIDKGLDSD
                     LQTGLEIERMLFSGLFATEDRQAGMDSFVEKGPGKATFTGR"
     gene            complement(6952..7515)
                     /locus_tag="PKU75_13040"
     CDS             complement(6952..7515)
                     /locus_tag="PKU75_13040"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPF81849.1"
                     /translation="MTDPTALPNLPGFGTNPAPSETGPAEAPLTERVTAALRELELEP
                     STDSDGDITFTFQDQQLFIRTSEDDAQILRVFGQWRLAEPVPTDPVERLRACNDVNIA
                     FNMIKTSMAEDTLLVTSEHMLPRGADVRSLLMVAIPLVLHGVSLWHQRAIGEEKLAEM
                     QAAETSGEATPGSAFGMNGSGPNGSGN"
     gene            7550..>7835
                     /locus_tag="PKU75_13045"
     CDS             7550..>7835
                     /locus_tag="PKU75_13045"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HPF81850.1"
                     /translation="MSVMSRELPPAPGMTLVPGGAEFAVYAAHATGVTLSLDDGHRER
                     RVELEGPLHGWWCADLPGVGPGTRYTLHADGPVDPGRSLRYDARTPLLDPY"
BASE COUNT         1160 a         2666 c         2867 g         1142 t
ORIGIN      
        1 cggccggcga tgatccgggc cgtgatgcgc tgcaggacat cgctcttcgt ctgtcccgtc
       61 acggattcga ggctgccgcg gatgaccacc gatcgatagt tggccgagga ctcgaaggtg
      121 gactcggcga cgaccaggcc gtcgacggcg gtgacgcaga aggcggccgg cgccccggac
      181 gcgacctgac gaagagctcc cgcgcccgtc gagccgtgca acaggatccg gtcgccgtcg
      241 cggccgtaga gcatcgggac gacccagggc tgcccgtcga ccaccgtggc gagggtgccg
      301 accagttggc cgtcgagcag gcggttcatc tcctcgcgcg tgcccaacgc gcgttcgggc
      361 ttgcgggtca ggcgggcgag acgatcggcg ggcgacggca taccaccatc ccaccacgtc
      421 cgcggcccgg gccggcgtca ggagtccgtg accgactccc ccgccgcagc gcgggccgaa
      481 cgttcggcga cggcatgggg gtcggtgcga gcgtcatacc gccagaaatc gcgcagcgcg
      541 gcggcggtca gcgccacgcc ggcgacgcac gcgagcccgc cgctggtgat ggcgccgcgg
      601 acgctccacg cgtcggcgac gaggccggcg cgagcctgcc cgccgagcgg gccgacggag
      661 aaggacagca tctcgatgcc ggccagacgc ccgcgcatgc gttcggggat ggtctggttc
      721 cagacggtgc cgcggaagac cgcggagaca ttgtccgcag cgcccgccag cgccagtgcc
      781 agcatcgcga gccagatcgt cggagcgaga ccgacgagcg cgatggcgca gccgtatgcc
      841 gtcgccgcga tcgtgatcgc ccggccgtgg tggtgcaccc ggtgcatcca ccccgacagg
      901 ctggtcgcga cgagggcgcc gacggtctcg gccgagtaga ggaggccgac gagttcgggc
      961 cgttcgaaga cgtccgtggc gaacgcgggg aagagcacga tcggcatcgc gagcagcatc
     1021 gccgcgatgt cgatgagata ggtgcccagc agatcccggc ggcgcacggc atacgtccag
     1081 ccctcggcga tgccccgcac cgacggcgcg gtcgtctcgc cgcggtgcgg ataggggcgc
     1141 atccgggcat acatggccgt ggcgaacagc atgccggcga agtccacgac gaagcaccag
     1201 ccgatcccga cagtggcgat gagcaggccg gcgatcgtcg gcccgacgaa gaggccggcc
     1261 tgcatcccga acgacgagag ggcgttggcc tggctgatct ggtcgtggcg aacagtcctg
     1321 ggcagcaacg attctcgcga cggtcgttgc accgcgccga cggagatgag cagtgcgccg
     1381 atgacgaaga tcacgacgat gctcggccgc tgccggaagg cgttggcggc cagggcggcc
     1441 atcaggacga cctgggcgat gccgcacgtg acgagcaggc gacggcgatc gacgtggtcg
     1501 gccagcgccc cgccccagag gccgaagacg atgagcggca cgagctcgac gacgcccatc
     1561 aggccgacca tggcgttgga gccggtctcg cgatagatca ggaacggcag cgccacatag
     1621 gagaccatgt gcccgagata gaagaccgtg cccgcgatga ggagcagccg gtagtcgcgg
     1681 gactcgcggg cgggggtgag gtcgacgcgc aggccggcga gccgtcgtcg ccaggtcatg
     1741 cgccggtcgt atgccgcgcg cgccccgcct cggcgatcgt cgccgagccg acgacgcgcg
     1801 tgccgtcgta gaggacgacg gactggccgg gcgcgacacc gcggatggtg cgtccgaggc
     1861 ggacgacgat ctcgtcgccc tcgacgtgcg cggtggcggg gatctcctcg ccgtgggcgc
     1921 gcacctgggc gcccaccgcg atgggaccac tcggcgccgg gccgcaccag agcgcgcgga
     1981 taccgcggat ctcgtcgacg ccgagcaggt cggcggtgcc gatcgtcaca gtgttggcgg
     2041 cgcggtcgat cgcggtgacg tagcgcgcct gcccgtccat cgagctgcgt tccaggccaa
     2101 ggcctttgcg ctgcccgatg gtgaagccgt aggcgccgtg gtggtgaccg accacctgcc
     2161 cgtcggcgtc gaggatgtcg ccgggctgct cccccagccg gtcggtgagc cacccgcgcg
     2221 tgtcgccgtc ggggatgaag cagatgtcgt aggagtcggg cttcttggcg atgaagaaac
     2281 cccgctcggc tgcctcctcg cggacccggg gtttggtggt atcgccgagg gggaagaacg
     2341 cgcggtcgag ttggtcgggg gtgaggacgc cgaggacgta ggactggtcc ttggcggcgt
     2401 cgacggcgcg gtgcagttgc cgtccgtcgg ggccgtcgac gatctgcgcg tagtgtccgg
     2461 tcgccacggc gtcgaacccg agggcgagcg ccttgtcgag gagcgccgag aacttgatct
     2521 tctcgttgca gcgcaggcac ggattggggg tgcggccggc ggcgtactcc gcgacgaagt
     2581 cgtccacgac gtcgcgctga aagcggtcgg cgagatccca gacgtagaac ggtatgccga
     2641 gccggtcggc cacccggcgg gcgtcgccgg cgtcctcgat ggtgcagcag ccgcgcgcgg
     2701 actcgcgaag cgttgcggca gaacgcgaca gcgccaggtg gaccccgacc acctcgtggc
     2761 cggcatcgag catgcgggcg gcggcgacgg cggagtccac tcccccgctc atggccgcga
     2821 cgacacgcat caggccgccc cgcgcgcggc ctgcgagcgc gccaccgcat cgggcagcac
     2881 ggcgaggacg gcgtcgacgt cctcctcggt gctggtgtgc cccaggctga aacgcagcgc
     2941 gccggcggcg tcggcgtcgc cgatgcggag tgccgtcagg acgtggctcg gcctcgtcac
     3001 gccggccgtg caggccgacc ccgccgagca ggagatcccg gccgcatcga ggacgaagag
     3061 caacgcatcg cgatcggcgc ccggaacccg caggtgcgca ttgccgggca gccggtcggc
     3121 ggcactcccc ggagtccagg cgcccgtgac ctcgatgccg aggccgagcg cgagtgcccc
     3181 gcgaatgagc ttgtcccgca acgcaatgac gcgggtcatc tcgtcgtcca ggccggccac
     3241 cgcggtctcg accgccgccg cgagcgcgtg taccccgcgc gcgtcgagcg tgcctgagcg
     3301 caccttgcgc tcctgtccgc cgccgtgcaa ctgcggcgcg agcgcggcgt cccggcgagc
     3361 gagcagcgcg ccgctgccga ccggtccgcc gagcttgtgt ccggtgagcg tgagcaggtc
     3421 gagcccgctg gcggagaagt cgaccggcac atgcccgacc gcctgcacgg cgtcggagtg
     3481 catcggtatg ccgagctccc ggcatacggc cgccagctgc gcgatgggct ggatcgtccc
     3541 gatctcgttg ttgccccaca tgatgctcac cagcgccacg tccgcgccgt caccgagcgc
     3601 cgacgtgagc gcgtccggcg cgatggcgcc caccaccgtg ggctccacga aaaccgccgt
     3661 ggcgcccgag gactccgtca tgtaatcgac cgagtcgagg atcgcgtggt gctcgatggc
     3721 gctggtgacg actcgggtgc gcgccggctc agcagtatgc cgcgcggcat acgtcccttt
     3781 gaccgccagg ttgtccgcct cggtcccccc ggaggtgaag atgacctccg acggatgcgc
     3841 accgagggcc gccgcgatcc gctcgcgcgc ctcctcgact atccgccgcg ccgcccgacc
     3901 ggcggcgttg accgccgacg gattgccgac gagttcaaga gtctcgatca gggcagcccg
     3961 cgccgccggc accagcggtg tcgtggcagc gtgatcgagg tagtgcaccg agagattcta
     4021 cgagcgagat ctcggcggcc cggaatcgac gtctcgacga aggcaggcgg gacgagaagc
     4081 aaagccgggc gaggcggggg cgagagggcg gttgagaccg aaggtcgctc tgcgaccgag
     4141 tgtcgaatct cccgccccga cgccgccgcc tcgtccggcg agcacaagac gacgcgaact
     4201 agcccttcga ggcctggacc ttctccgtcg cctgcgggag gacggtgaag aggtcgccga
     4261 cgacgccgaa gtcgaccagt tcgaagatcg gcgcctccgg gtccttgttg atcgcgacga
     4321 tggtcttcga cgtctgcatg ccggcgcggt gctggatggc cccggagatg ccgcacgcga
     4381 catacagctg cgggctgacc tgcttgccgg tctggccgac ctgcgaggtg tggggatacc
     4441 agccggcgtc gacggcggcg cgcgaggcgc cgacggcagc gccgagggag tcggcgaagg
     4501 tctcgacggg cgagaagtcg ccgccggtgc cgcggccgcc ggagaccacg atcgcggcct
     4561 cggtcaactc gggacggccg gaggcctgct tctccttggc ctcggtgatc tgcgcggtct
     4621 tcgcggcgtc ggagatagtc aggttgaccg ggacgacctg cccggctgcc ggcttctcct
     4681 gcggcgcagc cgaattcggc ttgacgcaga tgatcggggt gcccttggtg accttggcct
     4741 tgacggtgta ggagccggcg aagacggact tcgtcgtctc gatgccgccg tcaccgggct
     4801 ggacgtcaac ggcgtcggtg atcaggccgg agccggtctt gacggcgacg cgggcggcga
     4861 tctccttgcc ggcggggttg gacgggatga ggacggcgcc gggcttgagc ttgccgacca
     4921 gtgcggcaag gaactcggcc tgcggcacga cgaggtagcc ggtcaggtcg gcgctgtcca
     4981 tcgcgtagac gcggccggca ccgaacttgc ccagggtcgc ggcggcctcg gcggcgcccg
     5041 gaccgaggta gacggcggac ggctcgccca ggcgggcggc gatcgtcagc atctcggcgg
     5101 tcgtcttgcg gacctgcccg ttcgcgtggt ccacgagcac gaagatttca gccattggtt
     5161 actcctggaa atgaaggtct cgtgggtcag atgaacttgc ggccggccag gaactcggcg
     5221 agcttggtgc cgccgtctcc ctcgtccgtg acgatctcgc cctgctcacg cggcgggcgc
     5281 ttggcgacgg agatcacctc ggtcgaggag cccgcggcgc cgacctgtcc ggcgtcgatg
     5341 ccgaggtcgg caagggtcca ggtctcgacc ggcttcttct tggcggccat gatccccttg
     5401 aaggaggggt agcgcggctc gttgatctgg tcagtgacgg agatcagggc cggtacggcg
     5461 ccgacgatgg tctcgctggc ggtgtccccg tcgcggcggg cggtgacggt gccgccgtcg
     5521 accgtcagct ccgaggcata ggtcacctgc ggcaaagcga gccgctcggc gagcatcgcc
     5581 gggaccacgc ccatggtgcc gtcggtcgag gccatgccgg tcagcaccag gtcgggcatg
     5641 ccgaccttct tgatggcctc ggcgaggatg agcgaggtgg ccagggcgtc ggagcccttg
     5701 atggcctcgt cgttgacgtg gacgcccttg tcggcgccca tctgcagggc cttcttgacg
     5761 gcgtcggccg cctgatcggg gccgatggtg atgacggtga ccgcaccctc gccggactcg
     5821 gcgatcttca gcgcctcctc gacggcgtat tcgtcgagtt cggacagcag cccgtccacg
     5881 ctctcgcggt tggtggtcag gtccgcctcg tcgaagcccc ggtccgcctg ggcgtccggc
     5941 acgtacttca cacaaacgac gatgttcatc tcgcgttgca tccttaacgg ttggtctggc
     6001 cggcagatcc cggcgcgggc actgcggttc agtctctcag gcaccgctcg gcgaaccggt
     6061 cccgtcgcgg cgtgattgtc aacacgccca gcgtgtcaag agtgcgcgac gcgccgtaat
     6121 gccggcggaa tgccggcgcg tcgcgcactc tcgacggggg gtgttcagcg gccggtgaag
     6181 gtggccttgc ccgggccctt ctcgacgaag gagtccatgc cggcctgccg gtcctcggtc
     6241 gcgaacagtc cggagaagag catccgctcg atctccaacc cggtctgcag gtcgctgtcc
     6301 agacccttgt cgatggcttc cttggccgcc cgcagcgcca gggcggggcc gccgacgaac
     6361 cgggacatcc agtcgcgggc ggcggcatac acctggtcgg cgggcaccac cttgtcgacg
     6421 aggccgatcg cgagcgcctc ggcggcgtcg acgaaccgcc cgctgtagac gatctccttc
     6481 gcccgcgccg ggccgacgag ccgggccagg cgctgggtgc cgccggcgcc ggggatgatg
     6541 ccgagcagga tctcgggctg cccgagcttg gcgtcctgcg ccgcgatccg gaagtcgcac
     6601 gcgagagcga cctcacatcc cccgcctagc gcatagccgg tgatcgcggc caccgtcggc
     6661 ttcggcaccg cggccagcgc gcgcgtgaag ccctggagcc cacccgccgc ggtggccatg
     6721 tcggcatacg acatctgctg catctgcttg atgtcggcgc cggcggcgaa gaccttctca
     6781 ccgccgtaga cgatcaccgc agctacggcc gggtcggccg acgccgccgc ggcggcctcc
     6841 ccgagctcgg actggacctg caggttcaac gcgttcatcg gtggccgggt caggcggatc
     6901 gtgccgatgc cgtcctcgac ctcgagggtg acgaattcgc ccatgacgca cctagttccc
     6961 gctgccgttg gggccggagc cgttcatccc gaaggccgac ccgggggtgg cctccccaga
     7021 ggtctcggcg gcctgcatct cggcgagctt ctcctccccg atggcccgct gatgccacag
     7081 cgaaaccccg tgcaaaacaa ggggaatggc caccatgagc aggctgcgga cgtcggcccc
     7141 gcgcggcagc atgtgctcgg acgtgacgag caaggtgtcc tcggccatgg aggtcttgat
     7201 catgttgaaa gcgatgttga cgtcattgca cgcccggagc cgctcgactg ggtcggtcgg
     7261 cacgggttcg gcaagacgcc actgaccgaa cacgcgcagg atctgggcgt cgtcctcgct
     7321 cgtgcggatg aacagttgct ggtcctggaa ggtgaaggtg atgtcgccgt cggagtcggt
     7381 gctcggctcc agttcgagtt cgcgcagcgc ggccgtgacg cgttcggtca ggggcgcctc
     7441 ggcggggccg gtctcggagg gtgccgggtt ggtgccgaat ccgggcaaat tgggcaatgc
     7501 ggtgggatca gtcattgctc caacctatcc atgcttaggc tagatgttca tgtcagtcat
     7561 gtcgcgggaa ctgccacccg cccccgggat gacgctggtg cccggaggcg ccgagttcgc
     7621 cgtatatgcc gcacacgcca ccggggtgac cctcagcctc gacgacggcc accgggagcg
     7681 ccgggtggag ctggagggcc cgctgcacgg ctggtggtgc gccgacctgc ccggagtcgg
     7741 accgggcacc cgttacacgc tgcacgccga cggacccgtc gatcccggcc gctcgctgcg
     7801 gtatgacgcc cgcacccctc tcctggaccc ttacg
//
LOCUS       DAOPJG010000162         7820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9924_length_7820_cov_8.722086, whole genome shotgun sequence.
ACCESSION   DAOPJG010000162 DAOPJG010000000
VERSION     DAOPJG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9924_length_7820_cov_8.722086"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1108
                     /gene="tilS"
                     /locus_tag="PKU75_13050"
     CDS             170..1108
                     /gene="tilS"
                     /locus_tag="PKU75_13050"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPF81851.1"
                     /translation="MAALADLAPDDLVLVACSGGTDSLSLVAATAWAAERLSLRAGAV
                     CVDHGLQAGSPQVAARAAEACRRLGLDPVEVVPVEVPADSPDGPEAAARLVRYAALAT
                     CADRLGAVAVLLGHTSDDQAEQVLLGLVRGSGIRSLAGIPPRRGEVRRPFLEISRAQT
                     HASCDELGLVPWQDPHNDDPAYLRVRVRRALIDLTTDLGPGVRAGLVRTAALARQDAD
                     YLDALAADARRALGDLPWEVGDLIRLDPALRTRVWRAALTDAGVPAGALGRNHIAECE
                     RLLTAWAGQGPIPLPGGYAVTRRSGQVSVSGPHTVN"
     gene            1114..1665
                     /gene="hpt"
                     /locus_tag="PKU75_13055"
     CDS             1114..1665
                     /gene="hpt"
                     /locus_tag="PKU75_13055"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPF81852.1"
                     /translation="MDAEHMGADLERVLITEEEIQARLAELAREIWDHYAGEEFLLVG
                     VLKGAVLVMADLMRALPGSAPMDWMAVSSYGSGTKSSGVVRILKDLDTDIMGRHVLIV
                     EDIIDSGLTLSWIRANLESRQPKSVEICTLLRKPEAAKVEIDCMWVGFDIPNDFVVGY
                     GLDYAEKYRNLRVVGTLAPHVYS"
     gene            1919..3976
                     /gene="ftsH"
                     /locus_tag="PKU75_13060"
     CDS             1919..3976
                     /gene="ftsH"
                     /locus_tag="PKU75_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPF81853.1"
                     /translation="MAMLTLMVVMFSLGGDGGYTRIDTSAATALLNEGKVESARFSSE
                     NVLDLTLKQGQTYSGEGVKAADKLRTEFIDARAEGLVTLVNEKVPGGYNDTIEHPSLL
                     SSLLVSILPLIILVGLFWFMMSQMQGGGSRVMQFGKSRAKLASKDTPKVTFADVAGAD
                     EAVEELQEIKEFLAEPAKFLAVGAKIPKGVLLYGPPGTGKTLLARAVAGEAGVPFYSI
                     SGSDFVEMFVGVGASRVRDLFEQAKANAPAIVFVDEIDAVGRHRGAGLGGGHDEREQT
                     LNQLLVEMDGFDVKTNVILIAATNRPDILDPALLRPGRFDRQIAVEAPDMIGRHRILE
                     VHAQGKPMAPGVDLLAVARRTPGFTGADLANVLNEAALLTARSSGHLITDATLDEAID
                     RVMAGPQKRTRIMSAKERKITAYHEGGHALVAAAMNHTDPVNKITILPRGRALGYTMV
                     LPSDDKYSTTRNEILDQLAYALGGRVAEEIIFHDPTTGAANDIEKATAMARKMVTEFG
                     MSERVGAIKLGQSQGEVFLGRDYGHQRDYSEHVAGIVDEEVRRLIEAAHDDAWHALND
                     NREILDNLVLELLERETLNHKDIAEIFRGVRKRPERARWLSAEHRLLSDKPPVLTPAE
                     QAALNGSGAPAGAIPRPGDRATEDAIDRAAEVGASPYAQDTPVTQVIETPESAPVDPD
                     GHV"
     gene            3987..4574
                     /gene="folE"
                     /locus_tag="PKU75_13065"
     CDS             3987..4574
                     /gene="folE"
                     /locus_tag="PKU75_13065"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005504347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPF81854.1"
                     /translation="MTEPADKVDQPRIEAAVREILAAIGEDPGRDGLLDTPARVARAY
                     AEAFGGLHADPAEVLAKKFEVDHQELVVVRDIAMYSTCEHHLLPFHGVAHIGYLPQGG
                     VVTGLSKLARLVDVFARRPQVQERMTGQIADALVEHLGVMGCIVVIEAEHLCMSMRGV
                     RKPGARTITSAVRGSLARNAKTRAEAMSLLYSGQR"
     gene            4589..5377
                     /gene="folP"
                     /locus_tag="PKU75_13070"
     CDS             4589..5377
                     /gene="folP"
                     /locus_tag="PKU75_13070"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPF81855.1"
                     /translation="MGVVNVTPDSFSDGGSWFDPDTAVAHGRLLAAQGADLIDIGGES
                     TRPGAVRPDEAEELRRVVPVVEALAGDGLTVSVDTMRAGVAAAAVAAGARIVNDVSGG
                     LADPAMAAVVADTDAEFVAMHWRGHSGDMYARARYDDVVADVCRELGDRVAALTAAGI
                     APERIILDPGLGFAKVPEHNWALLRALPTLGDLGHRVLVGTSRKRFLALVGRTQETMR
                     GTDERDVATAVTSAYAAQAGVWGIRVHDVVATVDALDVWEAMNG"
     gene            5370..6257
                     /gene="folK"
                     /locus_tag="PKU75_13075"
     CDS             5370..6257
                     /gene="folK"
                     /locus_tag="PKU75_13075"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPF81856.1"
                     /translation="MADSADRITLTGLRATGFHGVFDHEKRDGQEFVVDIVLGTDHRA
                     AGRSDALADTINYATVADLAMARITGPAYDLIEALAEAIAGDCLTLAGVHTVEVTVHK
                     PAAPIPHTFADVSVTVARGLEHPFVVALGSNLGDREATLRAAVQQLGFEPEIRIGRVS
                     GLVETDPVGGPEQSDYLNAVATGTTSLTAPDLLATLHRIEARHGRTRTVRWGERTLDL
                     DLIQFGTPGAPDEVRSSDPALLVPHPRAAERAFVVLPWLDADPRGRIRVGEDVIPLAL
                     LAAQLDPSGVRPLGGGSDD"
     gene            6250..6726
                     /locus_tag="PKU75_13080"
     CDS             6250..6726
                     /locus_tag="PKU75_13080"
                     /inference="COORDINATES: protein motif:HMM:NF022819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HPF81857.1"
                     /translation="MTERGMPWWVPAVFAAFVAAIVTLLTHRYVQDGHFPPSVPVAAV
                     ILTVVLALVVGWFGWGVRRYLGGNKADLNPIRAARTLALAQAGALTGAALFGLYAGQA
                     LGLLPERDLQASQRILWSLLWAILAAALLLGSGLLAQHWCRIPPRDRDAESERSGS"
     gene            complement(6832..7329)
                     /locus_tag="PKU75_13085"
     CDS             complement(6832..7329)
                     /locus_tag="PKU75_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPF81858.1"
                     /translation="MSAFDPETWLAPIDQQLWTFIDDHRAMLASCLDGLTEEEARVSL
                     VPSLTTLLGLVKHATFVERVWFDEAVTCRSRAEIGIPDTVDESFLLTDEDTIAAAQAA
                     YAAALADSRANVAGLGLDDIVTGNRRGPLPLRFVLIHVLRELAQHAGHADILREQVLA
                     ARDEA"
     gene            complement(7326..>7820)
                     /locus_tag="PKU75_13090"
     CDS             complement(7326..>7820)
                     /locus_tag="PKU75_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPF81859.1"
                     /translation="AHAYGVSGPIARASGVDVDLRRDAPYLAYAELFGPGGPGRVVTR
                     ETGDCLARLEVLLEQTHVALDLATACVDTLRTLPQGPINVKLPKVLKVPEGDLYTATE
                     NPLGFNGFYLVSTGEKTPHRLKLRSASFSNVQVLSQILPGCFIADMVAILGSMFFVVG
                     DVDK"
BASE COUNT         1160 a         2860 c         2647 g         1153 t
ORIGIN      
        1 actccctggc acatatctgc cggagtccgg gctacgccag gcagttcgcg gcataccgaa
       61 gagttcgagc gcgataggcg gccgacccgg ggcgtcaggc ctgaccgggt cgcggcatag
      121 gctcggggcc gtgcgggaac cggcgagtat ggcgtgtcgg ggggcggtca tggcagccct
      181 cgccgacctc gcacccgatg acctggtcct ggtcgcctgc tccggcggca ccgactccct
      241 ctcactcgtc gcggccacag cgtgggcggc ggagcggctg agcctgcggg ccggggcggt
      301 gtgcgtcgac cacggcctgc aggccggctc gccgcaggtc gccgcacgtg ccgcggaggc
      361 gtgccgccgg ctcggcctcg acccggtcga ggtcgtcccc gtcgaggtgc cggccgattc
      421 tccggacggg ccggaggccg ctgcgcggct ggtgcggtat gccgccctgg ccacctgcgc
      481 ggaccggctc ggcgccgtcg cggtgctgct cgggcatacc agcgacgacc aggccgagca
      541 ggtgctgctc gggctggtgc gcggctccgg catccgctcg ctcgccggga tcccgccgcg
      601 gcgcggcgag gtgcgccggc ccttcctgga gatcagccgc gcccagacgc acgcctcgtg
      661 cgacgaactc ggcctggtcc cgtggcagga cccgcacaac gacgacccgg cctacctgcg
      721 cgtgcgggtg cgccgcgccc tcatcgacct gaccaccgac ctcggccccg gcgtccgcgc
      781 cggcctggtg cgcaccgccg ccctggcccg ccaggacgcc gattatctgg atgccctcgc
      841 ggcggacgcg cggcgagcgc tcggtgacct cccgtgggag gtgggcgacc tcatacgact
      901 cgatccggcc ctgcgcaccc gcgtctggcg tgccgccctg accgacgcgg gcgtccccgc
      961 cggggccctc ggccgcaacc acatcgccga atgcgagcgg ctcctcaccg cgtgggcagg
     1021 gcaggggccg atcccgctgc ccggagggta tgccgtgacc cggcgcagcg ggcaggtgtc
     1081 ggtgagcggt ccccatacgg ttaactaacg tccgtggacg ctgagcacat gggtgcagac
     1141 ctcgaacgcg tactcatcac cgaggaggag atccaggccc ggctcgccga gctcgcccgg
     1201 gagatctggg accactacgc aggcgaggag ttcctgctcg tcggggtcct caagggtgcg
     1261 gtgctcgtca tggccgatct gatgcgcgcc cttcccggca gcgccccgat ggactggatg
     1321 gcggtcagct cctacggctc gggcaccaag tcgtccggtg tcgtgcggat cctgaaagac
     1381 ctcgacaccg acatcatggg ccgccatgtg ctgatcgtcg aggacatcat cgactccggc
     1441 ctgaccctgt cgtggatccg cgccaacctg gagtcccggc agccgaagtc ggtcgagatc
     1501 tgcaccctgc tgcgcaagcc ggaggcggcc aaggtcgaga tcgactgcat gtgggtcggc
     1561 ttcgacatcc ccaacgactt cgtcgtcggc tacggcctgg actacgccga gaagtaccgc
     1621 aacctgcgcg tcgtgggcac tctggcgccg cacgtctact cctgaccctg ggagcccggc
     1681 ttgccgttgc gtcagttcga ctggcccgga acaagcgggc cgccgtcggt cgttggccct
     1741 gacggatgaa cccgcgtccg tcttccagcg cgttgccggt gtagtttcgt ccccacgaac
     1801 gacccgtccc accggtcgcg ccttccgcgc gcccggccag cgcaaggagc ccggcctctt
     1861 ggccaagaat tcgccctcga acgccaagcg tctcgtccgc aaccccctgt tgtgggtgat
     1921 ggccatgctc accctcatgg tcgtcatgtt ctccctggga ggcgacggcg gctacacgcg
     1981 catcgacacc tcggccgcga cggccctgct gaacgagggc aaggtcgagt cggctcgctt
     2041 ctccagcgag aacgtcctgg acctgactct caagcaggga cagacctact ccggtgaggg
     2101 cgtcaaggcc gccgacaagc tgcggacgga gttcatcgac gcgcgcgccg aggggctggt
     2161 caccctggtc aacgagaagg tgcccggcgg ctacaacgac accatcgagc acccgagcct
     2221 gctcagttcg ctgctcgtct cgatcctgcc gctgatcatc ctcgtcgggt tgttctggtt
     2281 catgatgagc cagatgcaag gcggcggctc ccgcgtcatg cagttcggca agtcccgcgc
     2341 caagctcgcc tccaaggaca ctcccaaggt caccttcgcc gatgtcgccg gcgccgacga
     2401 ggccgtcgag gagttgcagg agatcaagga gttcctcgcc gagccggcga agttcctcgc
     2461 cgtcggcgcc aagatcccca agggggtgct cctctatggc ccgcccggca ccggcaagac
     2521 cctgctcgcc cgggccgtgg ccggcgaggc gggagtgccg ttctactcga tctccgggtc
     2581 ggacttcgtc gagatgttcg tcggtgtcgg cgcctcccgg gtgcgtgacc tcttcgagca
     2641 ggccaaggcc aacgcacccg ccatcgtctt cgtcgacgag atcgacgccg tcggccgcca
     2701 ccgcggcgcg ggcctcggcg gcgggcacga cgagcgcgag cagacgctca accagttgct
     2761 cgtcgagatg gacggtttcg acgtcaagac caatgtcatc ctcatcgcgg ccaccaatcg
     2821 gcccgacatc ctcgacccgg cgctgctgcg cccgggccgt ttcgaccggc agatcgccgt
     2881 cgaggccccc gacatgatcg gtcggcaccg catcctcgag gtgcacgccc agggcaagcc
     2941 gatggccccc ggggtcgacc tgctcgccgt cgcccgccgc accccgggct tcaccggcgc
     3001 cgacctggcc aacgtcctca acgaggccgc cctgctcacc gcccgtagtt ccggccacct
     3061 gatcaccgac gcgaccttgg acgaggcgat cgaccgggtc atggccggcc cgcagaagcg
     3121 cacccgcatc atgagcgcca aggagcgcaa gatcaccgcc taccacgagg gtggtcatgc
     3181 cctcgtcgcc gcggcgatga accacaccga cccggtcaac aagatcacga tcctgccccg
     3241 aggccgtgcc ctcggctaca cgatggtgct gccgagcgac gacaaatact ccacgacccg
     3301 caacgagatc ctcgaccagc tggcctacgc cctgggtggg cgggtggcgg aggagatcat
     3361 cttccacgac ccaaccaccg gtgccgccaa cgacatcgag aaggccaccg cgatggcccg
     3421 caagatggtc accgagttcg ggatgtcgga gcgcgtcggc gcgatcaagc tcggccagtc
     3481 ccagggagag gtcttcctcg gccgcgacta cggccaccag cgcgactact ccgagcatgt
     3541 cgccgggatc gtcgacgaag aggtgcgccg cctcatcgag gcagcgcacg acgacgcctg
     3601 gcacgccctg aacgacaacc gcgagatcct cgacaacctg gtgctggagc tgctcgagcg
     3661 ggagacgctc aaccacaagg acatcgccga gatcttccga ggagtccgaa agcgtcccga
     3721 acgagccagg tggctctcgg ccgagcaccg tctgctgtcg gacaagccgc cggtgctgac
     3781 gccggccgag caggccgcgc tcaacggctc cggcgccccc gccggtgcga ttccccggcc
     3841 cggggaccgc gcgaccgagg acgccatcga ccgtgcggcc gaggtcgggg cctcgcccta
     3901 tgcccaggac acgccggtca cccaggtcat cgagacgccc gagagcgcgc ccgtcgaccc
     3961 cgacggccac gtctgagcgg ccggagatga ccgagcctgc cgacaaggtc gatcagccgc
     4021 gcatcgaggc ggccgtccgc gagatcctcg ccgccatcgg tgaggatccc ggccgcgacg
     4081 gcctgctcga cactcccgcc cgcgtcgccc gggcgtatgc cgaggcgttc ggggggttgc
     4141 acgccgaccc ggccgaggtg ctggcgaaga agttcgaggt cgatcaccag gaactcgtcg
     4201 tcgtgcggga tatcgcgatg tactccacgt gcgagcacca cctgctgccc ttccacggcg
     4261 tcgcccacat cggctatctg ccgcagggcg gtgtcgtgac cggcctgtcc aagctggccc
     4321 ggctcgtcga cgtcttcgcc cgccgcccgc aggtccagga gcggatgacc ggtcagatcg
     4381 ccgacgccct ggtcgagcac ctcggcgtca tgggttgcat cgtcgtcatc gaggccgagc
     4441 acctgtgcat gtcgatgcgc ggggtccgca agccgggcgc ccgcaccatc acctccgccg
     4501 tccgcggcag cctcgcgcgc aatgccaaga cccgcgccga ggcgatgtcg ctgctctaca
     4561 gcggccaacg gtgaaccgtc cgctggtgat gggtgtcgtc aatgtgacgc ccgattcctt
     4621 ctccgacggc ggttcctggt tcgacccgga caccgccgtc gcgcacgggc ggctgctcgc
     4681 ggcgcagggc gccgacctga tcgacatcgg cggcgaatcg acccgtcccg gagcggtgcg
     4741 ccccgacgag gccgaggagt tgcgccgcgt cgtccccgtc gtcgaggcgc tcgccggcga
     4801 cggcctgacc gtctcggtcg acaccatgcg cgccggcgtc gccgcggccg ccgtcgcggc
     4861 gggcgcgcgc atcgtcaacg acgtctcggg cgggctcgcc gaccccgcca tggccgccgt
     4921 cgtcgcggac accgacgccg agttcgtcgc catgcactgg cggggccact ccggagacat
     4981 gtacgcccgg gcgcgttacg acgatgtcgt cgcggacgta tgccgcgaac tgggtgatcg
     5041 cgtggccgcg ctgaccgcgg ccggcattgc gccggagcgg atcatcctcg accccggcct
     5101 gggttttgcc aaagtgccgg aacacaattg ggcgctgttg cgggcactgc cgaccctcgg
     5161 cgacctcggc caccgtgtcc tcgtcggcac ctcacgcaag cgtttcctcg cgctcgtcgg
     5221 ccgcacccag gagaccatgc gcggcaccga tgagcgcgac gtcgccactg ctgtcacgag
     5281 cgcgtacgcc gcgcaggccg gagtgtgggg gatccgcgtc cacgatgtcg tcgccaccgt
     5341 cgacgccctc gacgtgtggg aggccatgaa tggctgactc ggcagaccgc atcacgctga
     5401 ccggcttgcg cgccacgggt tttcacggcg tcttcgacca cgagaagcgc gacgggcagg
     5461 agttcgtcgt cgacatcgtg ctcggcaccg accaccgcgc cgccgggcgc agcgacgccc
     5521 tggccgacac gatcaactac gccaccgtgg ccgaccttgc gatggcgcgg atcaccggcc
     5581 ccgcctacga cctcatcgaa gccctcgccg aggccatcgc gggtgactgc ctgacgctgg
     5641 cgggcgtcca taccgtcgag gtgacggtgc acaaacccgc cgcacccatc ccgcatacct
     5701 tcgccgacgt cagcgtcacc gtagcccgag gcctggagca ccccttcgtg gtcgccctgg
     5761 gcagcaacct gggcgaccgc gaggcgacgc tgcgggctgc ggtgcagcag ttgggtttcg
     5821 agccggagat ccgcatcggc cgcgtgtcgg gtctcgtcga gaccgacccg gtcggcggac
     5881 ccgagcaaag cgactatctc aacgccgtcg cgaccggcac caccagcctc accgcgcccg
     5941 atctgctcgc gaccttgcac cgcatcgagg cccgccacgg ccgcacccgc accgtccggt
     6001 ggggcgagcg caccctcgac ctcgacctga tccagttcgg cacccccggc gcaccggacg
     6061 aggtgcgcag cagcgacccc gccctcctcg tgccgcaccc gcgcgcggcc gagcgtgcct
     6121 tcgtcgtcct gccctggctc gacgccgacc ctcgggggcg catccgggtg ggtgaggacg
     6181 tcataccgtt ggctttgctt gcggcgcaac tggatccgtc cggcgtgcgc cctcttggcg
     6241 ggggcagcga tgactgagcg cggtatgccg tggtgggtgc ccgccgtctt cgccgcgttc
     6301 gtcgcggcga tcgtcacgct gctcacccac cgctacgtcc aggacgggca cttcccaccc
     6361 tcggtgcccg tggcggcggt catcctcacc gtcgtgctcg ctctcgtcgt cggctggttc
     6421 ggctgggggg tgcggcgcta cctgggcggc aacaaggccg acctgaaccc gatccgcgcg
     6481 gcgcgcaccc tggcgctggc gcaggccggc gcgctcaccg gtgccgcgct gttcgggctg
     6541 tatgccggtc aagcactcgg cctgttgccc gagcgcgatc tgcaggccag ccagcggatc
     6601 ctgtggagcc tgctgtgggc gatcctcgcc gccgcgctgc tgctgggctc gggtctgctc
     6661 gcccagcact ggtgccgcat ccccccgcgc gaccgcgacg ccgaatccga acgctccggc
     6721 tcatagggcc gcccgggtcg atccgggcgc gggcgacgac atacgaaata cgagaggggc
     6781 gcagatccga aggccggatc ggtcaaacgg ctcgcttcgt attctgaatg ttcacgcctc
     6841 gtcgcgggcg gcgagcacct gctcgcgcag gatgtcggcg tggccggcgt gctgcgccag
     6901 ttcgcgcaac acgtggatga gcacgaaacg cagggggagc gggccgcggc ggttgccggt
     6961 cacgatgtcg tccaggccga gcccggcgac attggcgcgc gagtcggcga gggcggcggc
     7021 atacgctgcc tgcgccgcgg cgatggtgtc ctcgtcggta aggaggaagg attcgtcgac
     7081 ggtgtcgggt atgccgatct ccgcgcggga gcggcaggtc accgcctcgt cgaaccagac
     7141 cctctcgacg aaggtggcgt gcttgaccag gcccagcagg gtggtcaagg acggcacgag
     7201 cgagaccctg gcctcctcct cggtcaatcc gtcgaggcag gaggcgagca tcgcgcggtg
     7261 atcgtcgatg aaggtccaca gctgctgatc gatcggcgct agccaggtct cgggatcgaa
     7321 ggcgctcact tgtccacatc cccgacgacg aagaacatcg accccaggat ggccaccatg
     7381 tcggcgatga aacagccggg caggatctgc gagagcacct gcacattgct gaacgacgcc
     7441 gagcggagct tgagccggtg cggggtcttc tcacctgtcg agacgagata gaaaccgttg
     7501 aaccccaacg ggttttccgt cgcggtgtag aggtcgccct ccggcacctt gagcaccttg
     7561 ggcagtttga cgttgatcgg cccctgcggc agcgtccgca gcgtgtcgac gcaggccgtc
     7621 gccaggtcca aggcgacgtg ggtctgctcg agcagcactt ccagccgggc caggcagtcg
     7681 cccgtctccc gcgtcaccac ccggccgggc ccaccggggc cgaacagctc ggcatacgcc
     7741 aggtaggggg cgtcgcggcg caggtccacg tcgaccccgc tcgccctggc gatgggtccg
     7801 gacacgccat acgcgtgcgc
//
LOCUS       DAOPJG010000163         7793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_9977_length_7793_cov_7.329672, whole genome shotgun sequence.
ACCESSION   DAOPJG010000163 DAOPJG010000000
VERSION     DAOPJG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9977_length_7793_cov_7.329672"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PKU75_13095"
     CDS             <1..268
                     /locus_tag="PKU75_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81860.1"
                     /translation="ASLELGAETPKDRERLAAELRLQCYRWADAPEPAGGWRLQSPAA
                     GERSPRARAPRATAHRAPAPEPVAVYCPSCFVQLPATGVCDTCA"
     gene            complement(296..1828)
                     /locus_tag="PKU75_13100"
     CDS             complement(296..1828)
                     /locus_tag="PKU75_13100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPF81861.1"
                     /translation="MTAGTSGGDDLVLAVDLGSGGPKIGYVTLTGEPVWWWYERADAV
                     QGANVQDPEDWWRSITGIAAQAISSGAVDGSRVVGVACTGQWASTVPVDADGLPVGEC
                     LMWSDTRGAEHSAKRFGGPVAGYAARPLATWLRRSGGIPNPAGADPVAHMLHLQFDRP
                     EVEARWFLEPVDYLTMRFTGIAAATPASMTGAWLTDNRRPDNAAYDPVLVRMAQVDAD
                     RLPPLVPTGSIVGTVRPDVAALLGIPATARVVTGLPDLQNAAIGSGAVGLHQPHISLG
                     TSAWLSCPVPGKKTDVLRSMAAVPGIGDGNYLLANNQDNAGRALEWFKSAFAPDVPYA
                     ELFARAAQVPAGAGRALFTPWLSGERSPVDDRQARGGFHNLSLDADRDDLTRAVLEGV
                     ALNMRWLLAGCERFAGRRLDPIRLIGGAARNDLWCQILADVCDRSLERIRDPLVAGLR
                     GCGLTFALASGAVTVSEVGDLVPVDRFFDPDPEHREIYDLLYAQFPKLHSRNRTMFVA
                     LN"
     gene            complement(1825..3240)
                     /locus_tag="PKU75_13105"
     CDS             complement(1825..3240)
                     /locus_tag="PKU75_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPF81862.1"
                     /translation="MSDFPYAERFPVQRTLPEQGRPRSELLAELTAMAQEEDAVWESG
                     KISGTMYCGDHEHYAFLSEAFAKFAHVNALQRDMCPSQTKFEGEIIAMVLDLMHADAV
                     TDTTPAGLVTSGGTFSILHAILAYREHASATRGISTAAGRLPNLVKPETAHPAFDKAC
                     HLFGIENRTVPVDPATTLVDPSAMAARIDDNTIAIVGSAGNYPYGTIDPISELGRLAL
                     DRGVGLHVDGCLGGFILPFGEELGYGIPPFDFRVPGVTSISADTHKYGYAFKGSSVVV
                     FRDKAIRNSQYFHQLAWLGGKYMSPGMDGSRSGGLLAATWASMTHLGREGYRRYAGEI
                     FETAARLMDVVRSHPPLRIMGDPTFCFSFTSEDFDIYHVADFMKPRGWRFNGQQYPNA
                     IHLAVTRPQTKPGVVEAFSSDLADAAIYAQEQAAAGRMTRSGAIYGGVVGGLTSEVET
                     VMTTIMDGMLDTQQSLPPEVR"
     gene            complement(3302..4552)
                     /locus_tag="PKU75_13110"
     CDS             complement(3302..4552)
                     /locus_tag="PKU75_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81863.1"
                     /translation="MTVVLIALGSRGDVQPLATLAAEITRRGGSALVLAPDDFAGLCA
                     ELGADFVPLGADSRDAVDMTHARFGKQLFGSMWGQAWLLRRWVRTVAPVAARTAADAV
                     QPGDSVLAGVLARDLAASLAQTRGARPLTVLFTGQLPTAFRESHFGFEFFRESAAYNR
                     AGSRFGWRIANSLGLPLGNAVRRRAGIRTRRARRATSDADRHPVLIAASPLVVPTAPD
                     WPATARQTGYLTGPAPDVRAPAEVVDFLAAGKPPTYVGFGSLGPSGAHNDLGVVVAAS
                     RRSGIRIVTPAVGSARPGLVDEGVLAIDPIAHSWLFPRMAGVVHHGGAGTTWAGLRSG
                     VPSAAIPFGVDQPYHAHRLTSLGVGPDTFPVQQLSPESLAGLLAALTEGRYAARASEL
                     GTLARAEDGLGATLAYLDEAGYLG"
     gene            complement(4572..5471)
                     /locus_tag="PKU75_13115"
     CDS             complement(4572..5471)
                     /locus_tag="PKU75_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HPF81864.1"
                     /translation="MDVSLDQLRTLAAVIDEETFDAASAALGITPSAVSQRIKALESA
                     VGRVLVTRSKPVRVTPPGAAVLRLARVTTRLADDTANEIGIGATAGFATLPIAVNADS
                     LATWFLPALARMPARFRAVFDVHVDDQDRTFELFSSESVLAAVTSGARPVPGCTVTPL
                     GSMRYFACAATDFAEAHFPRGLTAQALATAPVVVFNRKDTLQHRFIADVAGHPLQPPA
                     HYLPASADFVHGIQLGLGWGLVPELQYAALPQRDSLVHLAPERPVDVPLHWQVWSVDS
                     APLRALTGVVTAAAHRSLDPARR"
     gene            5543..6154
                     /locus_tag="PKU75_13120"
     CDS             5543..6154
                     /locus_tag="PKU75_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HPF81865.1"
                     /translation="MDSPWSAALTGMLTGAALIIAIGAQNAYVLRQGIKRSHVLPIVA
                     ICALSDAVLIAAGVGGMGAIVAAAPGLITAIKWVGAVFLVGYGVLAARRALHGEHLDA
                     AGGLGPTSLKAAVLTALAFTWLNPHVYLDTLVFLGSVATSHAERRWWFWAGASAASVI
                     WFSGLGFGARLLGPLFAKPRAWRVLDGVIAALMVTLGVSLAAG"
     gene            complement(6159..7508)
                     /gene="gdhA"
                     /locus_tag="PKU75_13125"
     CDS             complement(6159..7508)
                     /gene="gdhA"
                     /locus_tag="PKU75_13125"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPF81866.1"
                     /translation="MPADLHPNFQSQYDAVIARNPGEPEFHQAIYEVMLSLAPIAGER
                     PEYAQWSILKRICEPERQIIFRVPWVTDDGEVRINRGYRVEFNSALGPYKGGLRFHPS
                     VNLSIIKFLGFEQTFKNSLTGMPIGGGKGGSDFDPKGRSNAEIMRFCQSFMTELYRHI
                     GEYTDVPAGDIGVGGRELGYLFGQYKRITNRYESGALTGKGLTWGGSRVRTEATGYGT
                     VFFAQQMLGTRGQSFDGKRVVVSGSGNVAIYAIEKVHQLGGTVIACSDSDGYVVDEAG
                     IDLDLLKDIKEVRRGRLTEYADQRDSARHVSGGSLWDVPCQVALPCATQNELPAAGAK
                     TLLDNGVELVAEGANMPSTPEAIGLFQEAGILYAPGKASNAGGVATSALEMQQNASRD
                     SWNFEDTEARLEQIMTDIHAVCVATAEEFGHPGDYVVGANLAGYIKVADAMVALGVI"
BASE COUNT         1237 a         2557 c         2802 g         1197 t
ORIGIN      
        1 cgcgtcgctg gagctcggtg cggagacccc caaggatcgc gagcgactgg cggccgaact
       61 gcggctgcag tgctaccgct gggcggatgc acccgagccg gcggggggct ggcggctgca
      121 gtcgcctgct gccggggagc ggtcgccgcg cgcccgcgcg ccccgggcca cggctcaccg
      181 agcacccgcg cccgaaccgg tggccgtcta ctgcccgagt tgcttcgtcc agctccccgc
      241 gacaggggtg tgcgacacct gcgcctgacc catcgcaggg cggacgccga tcggctcagt
      301 tcagtgcgac gaacatcgtg cggttgcgcg agtgcagctt cgggaactgc gcatagagca
      361 ggtcatagat ctcgcgatgc tccggatccg ggtcgaagaa tcggtccacc ggcaccaggt
      421 cgcccacctc gctgaccgtg acggcaccgc tggccagggc gaaggtcaac ccgcaaccgc
      481 gcagcccggc aacgagcgga tcacggatgc gttccaggga acggtcgcac acgtcggcca
      541 ggatctggca ccaaaggtcg ttccgggcgg ccccgccgat cagccgaatc gggtcgagcc
      601 ggcgcccggc gaagcgttcg cagccggcca ggagccatcg catgttgagg gcgaccccct
      661 cgagcacggc gcgcgtcagg tcgtcgcggt cggcgtccaa ggacaggttg tgaaaaccgc
      721 cgcgggcctg gcgatcgtcg acgggagagc gctcacccga gagccacggc gtgaagagcg
      781 cccggccggc gcccgcgggc acctgtgcag cgcgggcgaa caactcggca tacggcacgt
      841 cgggggcgaa agccgacttg aaccattcga gcgcccggcc ggcgttgtcc tggttgttgg
      901 caagcaggta gttgccgtcg ccgatgccgg ggacggccgc catggaccgc aggacatcgg
      961 tcttcttgcc cggcaccggg cacgagagcc acgccgaggt gccgagcgag atgtgcggct
     1021 ggtgcaaccc gaccgcgccg gacccgatgg cggcgttctg cagatcgggc agcccggtga
     1081 cgacgcgcgc ggtcgccgga atacccagca gcgcagcgac atccggacgc actgtgccga
     1141 cgatggagcc ggtcggcacc agcggcggca gccggtcggc atcgacctgc gccatccgca
     1201 ccagcacggg gtcgtatgcc gcattgtccg gcctgcggtt gtcggtcagc cacgccccgg
     1261 tcatcgacgc gggtgtggcg gctgcgatgc cggtgaagcg catcgtcagg tagtcgaccg
     1321 gttcgaggaa ccagcgcgcc tcgacctcgg gccggtcgaa ctggagatga agcatgtgcg
     1381 ccaccgggtc cgccccggca gggttgggta tgccgcccga gcggcgtaac cacgtcgcga
     1441 gggggcgggc ggcatacccc gcaaccggcc cgccgaagcg tttcgccgag tgttcggcgc
     1501 ctcgggtgtc gctccacatg aggcattcgc cgacgggcag tccgtcggcg tcgaccggca
     1561 ccgtgctcgc ccactgcccg gtacaggcaa cgccgacgac gcgggatccg tcgacggcgc
     1621 cggaggagat ggcctgcgcc gcgatgccgg tgatggagcg ccaccagtcc tccgggtcct
     1681 ggacgttcgc gccctggacc gcgtccgcgc gctcgtacca ccaccagacc ggctcacccg
     1741 tgagcgtgac gtagccgatc ttcgggccgc cgctgccgag gtcgacggcc aggacgaggt
     1801 cgtccccgcc ggaagtgccg gcggtcatct cacctcgggc ggcagcgact gctgcgtgtc
     1861 gagcatgccg tccatgatcg tcgtcatgac cgtctccacc tcactcgtca ggccgccgac
     1921 gaccccgccg tagatggcgc ccgaccgggt catccgacca gcggccgctt gctcttgcgc
     1981 atagatcgcc gcgtcggcca agtcggacga aaaagcctcc accacaccgg gtttggtctg
     2041 gggccgggtg acagcgaggt ggatcgcgtt ggggtactgc tggccgttga agcgccaccc
     2101 gcgcggtttc atgaagtcgg cgacgtgata gatgtcgaag tcctcggagg tgaaggagaa
     2161 gcagaaggtc gggtcgccca tgatccgcaa cggtgggtgg ctgcgcacga cgtccatcag
     2221 gcgcgcggcg gtctcgaaga tctcgccggc atagcggcgg taaccctccc ggcccaggtg
     2281 tgtcatcgac gcccaggtcg cggcgagcaa tcctccggaa cgcgatccgt ccatgccggg
     2341 cgacatgtac ttcccgccca accaagcgag ctggtggaag tactgactgt tgcgaatcgc
     2401 cttgtcgcgg aagaccacca ccgacgatcc tttgaaggcg tagccgtatt tgtgagtgtc
     2461 ggccgagatc gaggtcacgc ctggaacccg gaagtcgaag ggcggtatgc cgtaacccaa
     2521 ctcctccccg aaggggagga tgaaaccgcc gaggcagccg tcgacatgca gcccgacacc
     2581 tcgatccagg gccagtcggc cgagttcgga gatcgggtcg atggtgccgt agggatagtt
     2641 gcccgcggac ccgacgatgg cgatggtgtt gtcgtcgatc cgggccgcca tcgcggaggg
     2701 gtcgaccagg gtcgtggcgg gatcgactgg gacagtgcga ttttcgatgc cgaacaggtg
     2761 gcaggccttg tcgaatgccg gatgcgctgt ctcgggtttg acgaggttcg gcaggcgtcc
     2821 ggcagcagtc gagatgccgc gtgtcgcgga tgcgtgttct cgataggcga ggatcgcgtg
     2881 caggatgctg aaggtgccgc cgctggtgac caagccagcc ggggtggtgt cggtcacggc
     2941 gtcggcgtgc atcaagtcga gcaccatggc gatgatctcg ccctcgaact tcgtctgcga
     3001 agggcacata tcgcgttgca gcgcattgac atgcgcgaat ttcgcgaacg cctcggagag
     3061 gaaggcatag tgctcgtgat cgccgcagta catcgtcccg gagatcttgc cgctctccca
     3121 gaccgcgtcc tcttcctggg ccatcgcggt cagctcggca agcaactcgg agcgcggccg
     3181 gccctgctcg ggcagggtgc gctgcacggg gaagcgctcg gcatacggga agtcggacat
     3241 ctggcctcca ccatccgtca acggggtcga gagcgacgtt accagtgggt aagtgcaggg
     3301 gtcagccgag gtagccggcc tcgtcgaggt aggccagcgt ggcacccagc ccgtcctcgg
     3361 cgcgagcaag cgtgccgagc tcggaggcgc gagcggcata ccgcccctcg gtgagcgcgg
     3421 caaggagtcc ggccaagctc tccggcgaaa gttgttgcac cggaaaggta tcgggtccca
     3481 cccccagaga cgtcagacga tgggcgtgat agggctgatc gacgccgaaa gggatcgcgg
     3541 ccgagggcac gcccgagcgc aaccccgccc aggtcgtccc ggcgccgccg tgatggacca
     3601 cgcccgccat gcgcgggaag agccaggaat gggcgatcgg gtcgatcgcg agcactccct
     3661 cgtcgaccaa gccgggccgc gcgctcccga ccgcgggggt gacgatacgg atgccgctgc
     3721 gccggctggc cgccacgacg acgccgagat cgttgtgagc gccgctcggg ccgaggctgc
     3781 cgaagccgac ataggtggga ggcttcccgg cggcgaggaa gtcgaccacc tccgcgggcg
     3841 cgcggacgtc gggggcggga cctgtcaggt agcccgtttg ccgcgccgtc gccggccagt
     3901 ccggcgccgt aggcacgacg agcgggctgg cggcgatgag caccggatgg cggtcggcgt
     3961 cggaagtggc ccggcgcgcg cgtcgagtgc ggatgccggc gcgtcggcga acggcgttgc
     4021 cgaggggcag gccgaggctg ttggcgatgc gccagccgaa acggctgccg gcccggttgt
     4081 atgccgcgga ttcccggaag aactcgaacc caaagtgact ctcgcggaac gctgtcggca
     4141 gctggccggt gaagaggacg gtcagcggac gggccccgcg cgtctgcgcc agactcgccg
     4201 cgagatcccg ggccagcacg cccgccagca cgctgtcgcc cggctgcacg gcatcggcgg
     4261 cggtgcgcgc cgcgaccggg gccacggtgc gaacccaccg gcgcaagagc catgcctgtc
     4321 cccacatgct cccgaagagt tgttttccga accgggcgtg cgtcatgtcg acggcatccc
     4381 ggctgtccgc gccgaggggg acgaagtcgg cgcccagttc ggcacagagc ccggcgaagt
     4441 cgtcgggcgc gaggacgagc gcggagccgc cccggcgcgt gatctcggcg gcgagtgtgg
     4501 ccaggggttg cacgtcgccg cgtgagccga gggcgatcag caccaccgtc atgacgtgat
     4561 tcttccaacc gtcagcggcg tgccgggtcc agggaccggt gcgccgcagc ggtgaccacc
     4621 ccggtcagtg cgcgcaacgg cgcggagtcc acgctccata cctgccaatg aagagggacg
     4681 tcgaccggtc gctccggcgc gaggtgcacc agcgaatctc gctgggggag agcggcatac
     4741 tgcagctcgg ggacgagccc ccagccgagt ccgagttgta tgccgtggac gaagtccgcg
     4801 gacgcgggca gatagtgcgc gggcggttgg agcgggtggc cggcgacgtc cgcgatgaac
     4861 cggtgctgca gcgtgtcctt gcgattgaag accacgaccg gggccgtggc gagtgcctgt
     4921 gccgtcaggc cgcgcgggaa gtgggcctcg gcgaaatccg ttgcggcgca agcaaagtag
     4981 cgcatcgatc ccaacggggt gactgtgcac ccaggcaccg gtcgggcgcc agaggtgacc
     5041 gcggcgagga ccgactcgct gctgaagagt tcgaaggtgc ggtcttgatc atcgacatgg
     5101 acgtcgaaga cggcccgaaa gcgagcgggc atgcgcgcga gcgcggggag gaaccaggtg
     5161 gcgagcgagt cggcgttgac ggcgatcggg agggtggcga aaccggcggt ggcaccgatg
     5221 ccgatttcgt tcgccgtgtc gtcggcgagg cgcgtggtga cccgggccag ccgcaggacc
     5281 gccgcgccgg ggggcgtgac ccgcaccggc ttgctgcgcg tcacgaggac ccgcccgaca
     5341 gcactctcca gggccttgat gcgctggctc accgccgagg gagtgatgcc gagggccgcc
     5401 gacgcggcgt cgaaggtctc ctcgtcgatg acggcggcga gggtgcgcag ctggtccagc
     5461 gacacgtcca tacagcaagt ctaatgatta ataagaaaag tgagttggct taatggtgtg
     5521 aggggctttt agcgttgccg gcatggattc cccctggagc gcagccctga ctgggatgct
     5581 gaccggcgcg gccctgatca tcgcgatcgg cgcgcagaac gcctatgtgc tgcgccaggg
     5641 catcaagcgc agtcatgtgc tgccgatcgt cgcgatctgc gcgctctccg acgccgtgct
     5701 catcgccgcc ggcgtgggcg ggatgggggc gatcgtcgca gcggcccccg gcctgatcac
     5761 ggcgatcaag tgggtgggcg ccgttttcct cgtcggctac ggcgtgctgg cggcccgccg
     5821 ggctctgcac ggcgagcacc tcgatgccgc ggggggcctc ggcccgacct cgttaaaggc
     5881 ggcggtgctg acggcgctcg ccttcacgtg gctcaacccg cacgtctatc tcgacaccct
     5941 ggtcttcctc gggtcggtgg ccacgagcca cgcggagcgg cgttggtggt tctgggccgg
     6001 ggcgtcggcg gcgtcggtga tctggttctc cgggttggga ttcggtgccc ggctgctcgg
     6061 gcccttgttt gccaagcctc gcgcgtggcg cgtcctcgac ggcgtcatcg cggccctgat
     6121 ggtgaccctc ggggtgagcc tggcggccgg gtgagtgcct agatcacccc caaggcgacc
     6181 atggcgtccg cgaccttgat atagccggcg aggttggcgc cgaccacgta gtcgccgggg
     6241 tggccaaact cctctgccgt ggcgacacag accgcgtgga tgtcggtcat gatctgctcg
     6301 aggcgggcct cggtgtcctc gaaattccag gagtcgcggc tggcgttctg ctgcatctcc
     6361 agggcgctgg tggcgacgcc gccggcgttg gatgctttgc ccggcgcata caggatgccc
     6421 gcctcctgga agagcccgat cgcttcggga gtggacggca tgttcgcgcc ctcggcgacc
     6481 agctcgacgc cattgtccag caacgtcttc gcgcctgccg ccggaagttc gttctgggtg
     6541 gcgcagggga gggcgacctg gcacggcaca tcccacaagg acccgccgct gacatgccgg
     6601 gcgctgtcgc gctgatcggc atactcggtc agccggcccc gcctgacctc cttgatgtcc
     6661 ttgagcaggt cgagatcgat gccggcctcg tcgacgacat agccgtcgct gtcactgcac
     6721 gcgatcacgg tgccgccgag ttggtgcacc ttctcgatgg catagatcgc gacattgccc
     6781 gagccggaca cgacgacccg cttgccgtcg aaggactggc cgcgggtgcc gagcatctgt
     6841 tgggcgaaga agaccgtgcc atagcccgtg gcctcggtcc gcacccgcga gccgccccac
     6901 gtcaatccct tgccggtcag cgcgccggac tcgtatcggt tggtgatccg cttgtactga
     6961 ccgaacaggt agcccagctc gcgtccgccc acaccgatgt cgccggctgg gacgtcggtg
     7021 tactcgccga tgtgccggta tagctcggtc atgaacgact ggcagaagcg catgatctcg
     7081 gcattggagc ggcccttggg gtcgaagtcc gacccgccct tgccgccgcc gatcggcata
     7141 ccggtcaacg agttcttgaa ggtctgctcg aacccgagga acttgatgat cgacaggttg
     7201 accgagggat ggaagcgcaa tccgcccttg taggggccga gggccgagtt gaactcgacc
     7261 cgatagccgc ggttgatccg cacctcgccg tcatcggtga cccacggcac ccggaagatg
     7321 atctgccgct ccggctcgca gatgcgtttg aggatcgacc actgcgcata ctccggacgt
     7381 tcgccggcga tgggggcgag gctgagcatg acctcgtaga tcgcctggtg gaactccggc
     7441 tcaccggggt tccgcgcgat gacggcgtcg tactgcgatt ggaagttggg gtgcaggtcg
     7501 gcgggcatgt cctttacctt tccctatgac gcgtgctccc gggagtgtga tctcaccggg
     7561 gcctcacgaa aagcgcgaga ccaccaccag cccgtagccg atgcgcaacc ccagcccgag
     7621 acccgtgacg acgttcgagc agtgaggcgg aactgctgcc gaagtccgcg ctcgagcggg
     7681 tcttcttcga caacactctg gaggcctggt gtcagagcca gccgttctca tccgcgatgc
     7741 gctccgtcac gacctgtacc tgacattcgt cacgggtggt gcgaagtcgg gga
//
LOCUS       DAOPJG010000164         7782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_10002_length_7782_cov_6.444545, whole genome shotgun sequence.
ACCESSION   DAOPJG010000164 DAOPJG010000000
VERSION     DAOPJG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10002_length_7782_cov_6.444545"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PKU75_13130"
     CDS             <1..1228
                     /locus_tag="PKU75_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81867.1"
                     /translation="FYILTVTRWFPVGVIVGIFVLLATGRGLTTTQALTYAAASGIVT
                     FALELPTSGFADAFGRRAVYIAAAVVNVITAYAYVVAHSFWAFVGAATLMGIFRALDS
                     GPLEAWFVDEVHVSEPGKDVDQELSRQGTVLGLSIAAGSLVGGSLIWWHPIKSAPAIN
                     LSVWCFLGLNLIHVLAVVTLLKETPRHPVGARRRQLLAESVTGAPRVIADGLKLLRHN
                     HVLLGLMAAEVCWSIGMIAFESLLPLRLEEMVGTQQRAAALMGPVAAVGWLIFALGAW
                     LSGLTSRRIDIARTAVIGRLLNGLGVVVMGLVLGPVALIAVYFFTYSAHGFNGPPHAA
                     LLHREASAGNRATVLSMNSMMAFLAYGAIAPVAGWLSDRTSIALTTVVVGVVSVLGAI
                     FYVPARRAEREGRVAA"
     gene            complement(1238..1879)
                     /locus_tag="PKU75_13135"
     CDS             complement(1238..1879)
                     /locus_tag="PKU75_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF81868.1"
                     /translation="MRVVVAEDHALLRDGLTRLLTAYGLEVAEALDGTAGLPEALQRN
                     DVDAAILDVRLPPSFTDEGLRAAIEARRTRPGFPVMVLSQYVEHLYARELLASGEGAV
                     GYLPKDRVADAGEFVDSVRRVAAGGTVLDPEVVAALMHRRGDEPVDRLTEREREVLGL
                     MAEGRSNAAIGHALFVTEKAVAKHINSIFTKLDLPVDVDDNRRVLAVLAWLKV"
     gene            complement(1831..3069)
                     /locus_tag="PKU75_13140"
     CDS             complement(1831..3069)
                     /locus_tag="PKU75_13140"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPF81869.1"
                     /translation="MTSTRRSPWLGTGYGLGHVLMVVPLALLVAGASLIVGLSVVGLG
                     VLLVRPFAPALAGFAGVHRRMAASFLGRDLPEARLTRTGAGPWRQVIAWFGQRTFWQL
                     VLWLLFAATGGLALSALAAGLPFGIIALVATAFGLWSSGEAPLWVSLLLFALGIGLAP
                     LWWYYGDVFMRLRTHTEAAILRPDPNAVLEQRVADLTASRADTVDHSAAELRRIERDL
                     HDGAQARLVSLGMNLGMAADLLDRDPELARQLLQEARHATGAALGDLRSVVRGIHPPV
                     LADRGLIGAVQALALDLPLPVTVDAALPGRPAAPVESAAYFAVAECLANVVKHAGAQT
                     AWVVIAAPKNRLRLQVGDDGRGGVRLDRGTGLAGIARRLGAFDGTMDISSPDGGPTVV
                     TMEVPCEWSSPRTTLSSGTG"
     gene            3205..5403
                     /locus_tag="PKU75_13145"
     CDS             3205..5403
                     /locus_tag="PKU75_13145"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPF81870.1"
                     /translation="MSTLTAVPVRTARWSATHPWRAIGAWFAFVALAVALAMLVPTRS
                     TTDTDYGIGDSGRAHAMLADAGIDAPLTESVLITARSGALDPAQATQAAAELTDRMIR
                     APGVASVAPVEWSADRSAALVTVILDKGTDDAAPLRAQTAATGTQFPGLAIDQAGDLT
                     LDDGIFDRVGEDLRTAELISVPMTLLLMLLAFGALIAAGVPVLLAGTSVAATMGLAAP
                     ISHLVPAEPTVNSVIVLIGMAVGVDYSLFYLKREREERAKGARTLDAVEIAAATSGHA
                     ILVSGGAVIASMAGLYVVGAATFTSLATGAIAVVAVAVLGSLTVLPALLVKLGRFVDR
                     PRIPLLWRLNARIGRGGISRRLLAPVLRHPRMSLVASLVATIALAFPAVGMSLHSANL
                     TTLPADIPQVATFSRIVSAFPSQGTTAVVVARITSADQPGIEAALRRLTTDAVATGSF
                     TDLGHDPITVSADRTVTKLTVAIPFEEQDSRTRVAIDSLRESLAPKALGATGAEYAVG
                     GSAAASLDFADRQGTRMPWVIGFVLCLTGLMMIAAFRSLPLALVSAALNLLSIGVAFG
                     VLRLVFQDGHVESALGFTSPGFLIDWVPLFVLTILVGLSMDYHVFVLSRVREYAASGL
                     SAPDAVRHGISDTAGVVTSAAAVMVSVFAIFATLSMLEMKMMGVGLAVAILLDASIIR
                     LVMLPAILVLLGDRAWGRLVPREAESAQRAAERVPELVVAGPPQSAGVAH"
     gene            complement(5305..6183)
                     /locus_tag="PKU75_13150"
     CDS             complement(5305..6183)
                     /locus_tag="PKU75_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPF81871.1"
                     /translation="MTVDVIHRVRRVPEGNAKLFDDGQSISVGGPAANAARTVAALGA
                     RARLVAAYGDSPVTAFLAGTLADEGIQWVDPLAGSPNPTPVSSVLIDIGSGDRAVIAG
                     GTRTQTYPLAPNEWLDGVDAVLIDGHGGDLPAAVARAARQCGIPVVLDGGSYKETMSA
                     YLGDVDLALLSEDFLAPQGDSITWALRAGAWTAGRSAGPGAIDLDGALGRASIPVPSV
                     PVVDTLGAGDVLHGACAAALGALGDRQRILDAVSFASRVATASVGYPGALGWARDDQL
                     RDAFRRTLRRLGLPRH"
     gene            complement(6207..>7782)
                     /locus_tag="PKU75_13155"
     CDS             complement(6207..>7782)
                     /locus_tag="PKU75_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF81872.1"
                     /translation="GMIEIYKSWVDFGIDGFRIDTVKHVDLPFWQRFGPAIMEHAKAK
                     GRDDFFMFGEVYDTSPGTMSLYSTSGRLPGTLDFGFQSTAVDYAMGNSAKQLQELYAG
                     DNAYLDADSNAYSLTTFLGNHDMGRIGSFLVGASDDDVDLLKRDRLAMSLMYLSRGQP
                     VTYYGDEQGFVGWGGDKDSRQDMFTTRVEQYAEDAIIGGTPGARDYYGTDVPVYQHIA
                     ALTKLRADHPALANGIQIERFAADGEGVYAFSRIDRESNVEYLVAVNNAKDPQAVRVA
                     TATPGAAFASVFGSGEGATSGTDGSLEVTVPGREALVLKAGAAIPAALHPPTVTAVKA
                     GNGGPLTGQAKLTADVAPGAPVEVTFAGRPKGTGEWTFLGTDDNPAYGRYLDTSAVVP
                     GDYEVVAVARTLDGKVGYASASTTVAAGAEGGTQVTAPGSYQAKAGCSGDWQPDCTAT
                     TLTDPDGDGTYTLELTGLPAGDYEFKIAIGGTWDENYGGDGKKDGTNISFTATDGQPV
                     TISYDETTHRAAAASP"
BASE COUNT         1119 a         2739 c         2633 g         1291 t
ORIGIN      
        1 cttctacatc ctgacggtga ctcggtggtt ccccgtcggt gtgatcgtcg ggatcttcgt
       61 cctcctggcg actggccgtg gcctgaccac cacgcaggca ctgacgtatg ccgcggcctc
      121 gggcatcgtc accttcgccc tcgaactgcc gacgagcggc ttcgccgacg ccttcgggcg
      181 gcgggccgtg tatatcgccg cggccgtcgt caacgtcatc acggcctacg cctatgtcgt
      241 cgcccattcg ttctgggcct tcgtcggcgc cgcgacgctg atgggcatct tccgcgcgct
      301 cgactccggc ccgctggagg cgtggttcgt cgacgaggtg cacgtgagcg agcccggcaa
      361 ggacgtcgac caggaactct cgcgccaggg caccgtcctc ggcctgtcga tcgccgccgg
      421 ctccctcgtc ggcggcagcc tgatctggtg gcatccgatc aagagcgcac cggccatcaa
      481 cctgtcggtc tggtgcttcc tcggtctcaa cctcatccac gtgctcgccg tggtgaccct
      541 gctgaaggag acgccccggc atcccgtcgg cgcccgccgc cggcagctcc tcgctgagtc
      601 cgtcacaggg gcaccgagag tcatcgcgga cggcctgaag ttgttgcggc acaaccatgt
      661 tctccttggc ctgatggccg cggaggtgtg ctggtcgatc gggatgatcg cattcgaatc
      721 gctcctgccg cttcggctgg aagagatggt cggcacccag cagcgcgcgg ccgccctgat
      781 ggggccggtg gcggccgtgg gctggctgat cttcgccctc ggcgcctggc tgtccgggtt
      841 gacatccagg cggatcgaca tcgcccggac cgcggtcatc gggcgcctcc tcaacgggct
      901 cggtgtcgtc gtcatgggcc tggtcctcgg cccggttgcc ttgatcgccg tctacttctt
      961 cacctactcg gctcacggtt tcaacggccc gccgcacgcg gccctactcc atcgcgaggc
     1021 cagcgcaggc aaccgcgcga cggtgctgtc catgaactcg atgatggctt tcctcgccta
     1081 cggcgcgatc gccccggtgg cggggtggct ctcggaccgg acgtccatcg ccctgacgac
     1141 ggtcgtcgtc ggcgtcgtca gcgtcctcgg cgccatcttc tacgtcccgg cccgccgggc
     1201 cgagcgggag ggtcgagtcg ccgcctgagc gtcgtcgtca cactttcagc caggcgagga
     1261 cggccaggac ccggcggttg tcgtcgacat caacgggcag gtcgagcttg gtgaagatgc
     1321 tgttgatgtg cttggcgacg gccttctcgg tgacgaagag ggcgtgcccg atcgctgcat
     1381 tcgagcgccc ttcggccatc aaccccagca cctcacgctc ccgctccgtc aaccggtcga
     1441 ccggctcgtc gccacgccgg tgcatcagcg ccgcaacgac ttccgggtcc aggacggtgc
     1501 cgccggccgc tacacgccgc acgctgtcga cgaactcccc cgcgtcagcg acccggtcct
     1561 tgggcagata gcccaccgcc ccctcacccg aggcgaggag ctcacgggca tacaggtgct
     1621 cgacgtactg gctcagcacc atgacgggga atcccggccg ggtgcggcgg gcctcgattg
     1681 ccgcgcgcaa cccctcgtcg gtgaaggagg gcgggaggcg gacgtcgagg atcgctgcgt
     1741 cgacatcgtt gcgctgcaac gcttccggca ggccagcggt cccgtccaac gcctcggcga
     1801 cctccagccc gtaggccgtg agcaaccggg tcagcccgtc ccggaggaga gcgtggtcct
     1861 cggcgacgac cactcgcatg gcacctccat cgtcacgacg gtcggcccgc cgtcggggct
     1921 cgatatgtcc atggtgccgt cgaacgcgcc caatcggcgg gcaatccccg cgagtcccgt
     1981 gccacgatcc agtctgacgc cgccgcggcc gtcgtcgccg acctggagcc gcaaccggtt
     2041 cttcggcgcg gcgatgacca cccacgccgt ctgcgcgccg gcgtgcttga cgacattcgc
     2101 caggcactcg gcgaccgcga aataggctgc cgactccacc ggcgccgccg gccggccggg
     2161 cagggctgcg tcgaccgtca ccgggagggg caggtccagc gcgagggcct ggaccgcgcc
     2221 gatgagcccg cggtcggcga gcacgggcgg gtgtatgccg cgtacaaccg agcgcaggtc
     2281 gcccagagcc gcgcccgtgg cgtgacgggc ctcctgcagc agctggcggg cgagttcggg
     2341 atcgcggtcc aagagatccg cagccatccc gaggttcatc ccgagggaga cgagccgggc
     2401 ttgcgccccg tcgtgcaggt cgcgctcgat gcgccgcagt tccgcggcgg agtggtcgac
     2461 cgtgtcggcc cgggaggccg tcagatctgc aacgcgttgc tctagaacgg cgttcgggtc
     2521 cggtcgcagg atggccgcct cggtgtgcgt gcgcagccgc atgaagacat cgccgtagta
     2581 ccaccacagg ggtgcgaggc cgatcccgag cgcgaagagg agcagcgaga cccacagcgg
     2641 tgcctcccca gatgaccaga ggccgaacgc ggtcgcgacg agcgcgatga tcccgaacgg
     2701 caagccggcc gcgagggccg agagggccag cccgccggtc gcggcgaaca acagccacag
     2761 cacgagctgc cagaacgtgc gctgcccgaa ccaggcaatg acctgcctcc acggaccggc
     2821 gccggtccgg gtcagccgcg cctcgggaag gtcccggccg aggaagctgg ctgccatccg
     2881 ccggtgcacg cccgcgaacc cggccaacgc gggtgcaaac ggtcgcacga gaagcacccc
     2941 gagccccacc acggacagtc cgacgatgag gctcgcgccg gcgacgagca gggccaacgg
     3001 caccaccatc aggacatggc ccagcccgta tccggtgccg agccaaggag agcggcgagt
     3061 ggacgtcata gcggacatcc tcacccgtga cgtccggtcc cgtcaggggt gctagcacca
     3121 cttccacatc gggttccagg gctcccgaca tccgagccgt gagcgagcag gctcgacgcg
     3181 tccgcctcga caaccggagg aaatatgtcc accctcaccg ccgtccccgt ccgcaccgca
     3241 cgctggagtg cgacgcaccc gtggcgagcc atcggcgcat ggttcgcatt cgtcgccctt
     3301 gccgtggcgc ttgccatgct cgttcccacc cgatcgacga ccgacaccga ctacggcatc
     3361 ggtgactccg gccgcgcaca cgcgatgctc gccgatgccg gcatcgatgc cccgctgacc
     3421 gagagcgtgc tcatcaccgc gcgctccggg gccttggacc ccgcccaggc cacccaggcc
     3481 gcagccgagc tgaccgaccg catgatccgg gccccaggcg tcgcgtcggt cgctccggtc
     3541 gagtggagcg ccgaccggtc cgcggccttg gtgaccgtga tcctggacaa gggcacggac
     3601 gatgccgccc cgttgcgggc gcagacggcc gcgaccggca cgcagttccc cgggctcgcg
     3661 atcgatcagg ccggtgacct gaccctggac gacgggatct tcgaccgggt gggcgaggat
     3721 ctgcgtacgg ccgaattgat cagcgtccca atgactttgc tgttgatgct gcttgccttc
     3781 ggagctctca tcgccgcggg cgtacccgtc ttgctggcgg gcacctcggt ggccgcgacc
     3841 atggggttgg ccgccccgat ctcgcacctc gtcccggcag aacccaccgt caactccgtc
     3901 atcgtcctga tcggcatggc cgtcggcgtc gactactcac tcttctatct caaacgcgaa
     3961 cgggaggagc gcgccaaggg tgcacgcaca ctcgacgctg tggagatcgc cgctgccacg
     4021 tcgggacacg cgatcctcgt ctctggcggg gccgtcatcg cttcgatggc ggggctgtat
     4081 gtcgtcggtg ccgccacctt cacctcgctg gcgaccggcg ccatcgccgt cgtcgcggtc
     4141 gcggtgctcg gatcgctcac ggtgcttccg gcgctgctcg tcaaactcgg ccgcttcgtc
     4201 gaccggccgc gaattccatt gctgtggcgg ctgaacgcac ggatcggccg cgggggcatc
     4261 agcaggcggc tcttggcacc ggtccttcgt cacccccgga tgtctctcgt ggcgtcgctc
     4321 gtcgcaacca tcgccctggc ctttcccgcg gtcgggatgt ccttgcacag cgcgaacctg
     4381 acgaccttgc cggccgacat accgcaggtc gcgaccttca gccggatcgt ctcggccttc
     4441 ccgtcgcagg gcacgaccgc cgtggtggtc gcccgcatca cgtcggctga ccaaccaggg
     4501 atcgaggccg ctctgcggag gttgacgacg gacgccgtcg cgacaggttc cttcaccgac
     4561 ctcggccacg atcccatcac ggtctccgct gaccgcacgg tcaccaagct gaccgtggcc
     4621 attcccttcg aggagcagga ctcacgcacc cgggtagcca tcgattccct ccgagagtcg
     4681 ttggcaccca aagcactcgg cgccacgggc gcggagtatg ccgtcggcgg aagcgcagcg
     4741 gcatcgctcg actttgcgga tcgacagggc acgcgaatgc cctgggtgat cggcttcgtc
     4801 ttgtgcctga ccggactgat gatgatcgcc gcgttccgca gcctcccgct ggccctggtg
     4861 tcggcggctc tcaacctctt gtcgatcggt gtggccttcg gtgtcctgcg gttggtgttc
     4921 caggacgggc atgtggagtc cgccctcggg ttcaccagcc ccggattcct catcgactgg
     4981 gtcccgctct tcgtgctgac gatcctcgtc gggctctcga tggattatca cgtgttcgtg
     5041 ttgagccgcg tgcgcgagta tgcggcgtcg ggattgagcg ctcccgatgc ggtgcgccac
     5101 ggcatcagcg acaccgccgg agtggtgacc agcgcggccg cggtcatggt gtcggtgttc
     5161 gcgatcttcg ccacgctgtc gatgctcgag atgaagatga tgggcgtcgg gttggccgtt
     5221 gcgattcttt tggacgccag catcattcgt ctcgtcatgc ttcccgccat ccttgtcctg
     5281 ctcggcgatc gggcatgggg tcggctagtg ccgcgggagg ccgagtcggc gcagcgtgcg
     5341 gcggaacgcg tcccggagtt ggtcgtcgcg ggcccacccc agagcgccgg ggtagcccac
     5401 tgatgcggtt gcgacccggc tggcgaaact gacggcgtcg agaatgcgct ggcggtcgcc
     5461 gagcgcaccg agcgcggcgg cacacgcccc gtgcaacacg tcccccgcgc ccagtgtgtc
     5521 gaccaccggc acactgggca cggggatgga cgcgcggccc agcgctccat cgaggtcgat
     5581 ggcgcccggc ccagcagacc ggcctgctgt ccacgcccct gcgcggaggg cccacgtgat
     5641 cgagtccccc tgcggggcaa ggaagtcctc ggacagcagg gcgaggtcga catcgccgag
     5701 gtaggcggac atggtctcct tgtaactgcc gccgtcgagg accactggta tgccgcattg
     5761 ccgagcagcg cgggcgaccg ccgcgggcag gtcaccgccg tgcccgtcga tcagcaccgc
     5821 atccaccccg tcgagccatt cgttaggggc gaggggatag gtctgggtcc gggtgcctcc
     5881 cgcaatcacc gcgcggtcgc cggagccgat gtcgatcagc accgacgaca ccggcgtcgg
     5941 gttgggcgat cccgcgagag ggtcgaccca ctggattccc tcgtcggcca gggtgccggc
     6001 gagaaatgcc gtcaccgggc tgtcgccata ggcggcgacc agccgggccc gtgcaccgag
     6061 ggccgccacc gtgcgggcag cgttcgccgc cggcccaccg acagagatgg attggccgtc
     6121 gtcgaagagt ttcgcgttgc cctcgggcac gcgtcgcacg cggtggatca cgtcgacggt
     6181 cacgagtccg gcgcagagca ctctggtcac ggggatgcgg ccgccgctcg gtgagtggtc
     6241 tcgtcgtagc tgatcgtcac gggctgccca tcggtggcgg tgaaggagat gttcgtcccg
     6301 tcctttttcc catcgccgcc gtagttctcg tcccaggtgc cgccgatggc gatcttgaac
     6361 tcgtagtcgc ctgccggcag ccccgtgagt tccagggtgt aggtgccatc gccgtcgggg
     6421 tcggtcaatg tcgttgccgt gcagtccggt tgccagtccc cggagcagcc ggccttggcc
     6481 tggtagctgc cgggggcggt gacctgcgtg ccaccctcgg caccggccgc gacggtggtg
     6541 gaggccgagg catagccaac cttgccgtcc agggtgcggg cgacggcgac cacctcgtag
     6601 tcgcccggca cgacggccga ggtgtcgagg tagcggccgt aggccgggtt gtcgtccgtc
     6661 ccgaggaacg tccactcccc cgtgcccttt gggcgaccgg caaaggtcac ctccaccggc
     6721 gcacccgggg cgacgtccgc ggtgagcttg gcctgccccg tcaacgggcc cccatttccg
     6781 gccttcaccg ccgtcacggt gggcggatgc agtgcggcgg ggatggccgc tcccgccttg
     6841 agcaccagag cttcccgccc ggggaccgtc acctcgagcg acccgtccgt ccctgacgtc
     6901 gcgccctccc ccgatccgaa gacggacgcg aacgccgcgc caggggtcgc tgtcgcgact
     6961 ctgaccgcct gtggatcctt ggcgttgttg acggcgacga ggtactcgac gttgctctcc
     7021 cggtcgatcc gcgagaacgc atagacgcct tcgccgtccg cggcaaaccg ttcgatctgt
     7081 atgccgtttg ccagggccgg gtggtcggcg cgtagcttcg tcaacgcggc aatgtgttgg
     7141 tagacaggga catcggtgcc gtagtagtcc cgcgcccccg gtgtgccgcc gatgatcgcg
     7201 tcctcggcat attgctcgac gcgagtcgtg aacatgtcct gccgtgagtc cttgtcgccg
     7261 ccccagccga cgaagccctg ctcgtcgccg tagtaggtga cgggctgccc acgcgagagg
     7321 tacatcagcg acatcgcgag ccggtcgcgc ttgagcaggt cgacgtcgtc gtcggaggcg
     7381 ccgacgagga agctgccgat gcggcccatg tcgtggttgc cgaggaaggt cgtcagcgaa
     7441 taggcgttgg agtccgcgtc gaggtaggcg ttgtcgccgg catacagctc ctggagctgc
     7501 tttgccgagt tgcccatcgc gtaatcgact gcggtgcttt ggaatccgaa gtcgagcgtg
     7561 ccgggcagcc ggcctgacgt cgagtagagg gacatggtgc cgggtgaggt gtcgtagacc
     7621 tcgccgaaca tgaagaagtc gtccctgccc ttggccttgg cgtgctccat gatcgccggg
     7681 ccgaaccgct gccagaacgg caggtcgacg tgcttgacgg tgtcgatgcg gaagccgtcg
     7741 atgccgaagt cgacccagct cttgtagatc tcgatcatcc cg
//
LOCUS       DAOPJG010000165         7712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_10106_length_7712_cov_8.002612, whole genome shotgun sequence.
ACCESSION   DAOPJG010000165 DAOPJG010000000
VERSION     DAOPJG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10106_length_7712_cov_8.002612"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="gntA"
                     /locus_tag="PKU75_13160"
     CDS             <1..459
                     /gene="gntA"
                     /locus_tag="PKU75_13160"
                     /inference="COORDINATES: protein motif:HMM:NF041366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis heme-dependent
                     pre-guanitoxin N-hydroxylase GntA"
                     /protein_id="HPF81873.1"
                     /translation="IAVFREPVTPTEKDFEARLWQVLQQLHDEDTHPWADGVAADPEA
                     PQFAFSHAGRAYFIVGLHPRASRIARRAPLPTLVFNLHEQFEKLRAEGGFDRMRTAIR
                     RRDTKVQGSVNPMAADHGEASEARQYSGRRVEPTWQAPFSPKEIGDDRSG"
     gene            complement(540..1391)
                     /locus_tag="PKU75_13165"
     CDS             complement(540..1391)
                     /locus_tag="PKU75_13165"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPF81874.1"
                     /translation="MYTIKAVAEATGIPPATLRAWERRYAVVRPPRSPTGYRLYDESL
                     IQDLRVMRELVAVGHPASEAARIVAGRRQDVAAASPSPPISELIAAARSADPAVISAA
                     VAAGLAAPLPVAVSGWLVPALTAVGEGWVDGTVGIATEHLISHAIHRALATRLHRYTA
                     TARAAPVLVGLPSGSHHELGALAFAALAARDALPVRYLGADLPAAAWVQCARQTRAAA
                     AVIAVPTRVDVATTRRTTKALASVPAIRVFAGGAYQDDICETATPLGHDIVTAVSVLR
                     ARLESRP"
     gene            complement(1401..2666)
                     /locus_tag="PKU75_13170"
     CDS             complement(1401..2666)
                     /locus_tag="PKU75_13170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF81875.1"
                     /translation="MTAAPIARLWAWSRPATQPDDPRRARVRGRAVRLLTRRMADRAG
                     VRITLPDGSGMGPDCGDAPHLLVHDERIFDRIGIDFKIGVGEGFVAGEWGPGPGTDLA
                     DLLTRFAANLGDLVPPVLTRFRRLVEPRQPERHRNDRAGARANISHHYDLSNDAFAVF
                     LDETMTYSAAWFAPEDPAGPAGLATAQRRKVAGILDLANVGAGTEVLEIGSGWGQLAI
                     QAAERGALVHTITLSHEQQSLARERIAAAGFADVVDVELRDYRDIAGRYDAVVSVEMI
                     EAVGYEFWPRYFRSLADAVRPDGQVAIQAITMAHSRMLDSRHAYGWIHKHVFPGGLIP
                     SYAVIAHHASSAGLRVTARRSLRDDYARTLREWRKALLDNPGAVRAAGLDDRFLKVWE
                     FYLAYSEAGFRSGHLDVLQLRLTPRTADG"
     gene            complement(2663..3391)
                     /locus_tag="PKU75_13175"
     CDS             complement(2663..3391)
                     /locus_tag="PKU75_13175"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HPF81876.1"
                     /translation="MMRDTGAQLVRVRITHHRFSPIRHQVRFRGYLWLVDVAAIPRAR
                     WFAAILPRDHFGGQADSIVESLGEFLRTRGEAVRDGERVLMMTNARVAGHVFNPLTTY
                     FGVASDGRVRWVVLEIHNTYGERHAHLLHPDRHGRAVVAKEFYVSPFFPVSGEYAVIA
                     RLERDRVVLSITLRENSIRRFAATLVGDRVPATRARLLTAMATTPLITYQVSTRIRIH
                     GIWLWLRRLPVQIRPAHIPPEGFR"
     gene            complement(3388..4671)
                     /locus_tag="PKU75_13180"
     CDS             complement(3388..4671)
                     /locus_tag="PKU75_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF81877.1"
                     /translation="MNDVDNADRRPSVIVVGAGVSGLTAAHRLARTHRVTLVEADERL
                     GGHAHTHEVPTADGPLRIDSGFIVHNERTYPTFLSLLRDIGIATRPTEMSMSVTCDAC
                     GLSYAGGKGLGGILADPARVADRRFRALLQDVPRFHRAARDVLASGDRETTWGGFLAD
                     HGFSPYFVGHFAGPLVACVWSAGQRVALDYPAHHLFAFLDNHGMLSVTGSPTWRTIVG
                     GSARYVEALAALLPDIRTGAGAVAVRRLDREVEVRLADGSVLAADGLVLAVHADDART
                     ILTDATASEAAALEAIPYSDNHVVLHHDERVLPRRRRARASWNYRQSTCANLTQPARV
                     DYWMNRLHGLDGATQFIVSLNANDVDPFTYLAQMRYRHPVFTRESVAAAARLRDLGGP
                     RLAFAGAHLGWGFHEDGARSGLEAAERIAAATRWA"
     gene            4741..6099
                     /locus_tag="PKU75_13185"
     CDS             4741..6099
                     /locus_tag="PKU75_13185"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPF81878.1"
                     /translation="MTGVVWLRRDLRVRDHPALCAAAAEGPIVPLVVLDPVLTQDSPR
                     FESYLHAVRALREDLDGLVVRVGDPRYVVPAVCAEARARAVHVSAEVTPYGRHRDAAV
                     RLALARAGVEWIATGSPYAVTPGRVRTRAGMPFHVFTPFARAWRDSPVPSPPDPPARV
                     RYTRVVSADLPETGTATHPVPVTEAQARARWQEFLTDEIAGYGRDRDRPDHEATSRLS
                     TALKLGSIHPRTLLSDLRSRAATRSDGVERFITELAWREFYADVLWHHPRSAWHDLKD
                     SLHGMQYDHPETNPLAAQHLSAWQAGRTGYPFVDAGMRQLRAEGWMHNRVRMVTASFL
                     TKDLHIWWPHGARHFLDLLRDGDLASNSHGWQWVAGTGTDAAPYFRIFNPVTQGLRFD
                     PDGEYVRRYVPELAHLPGAAAHEPWKHPGPHVAGYPPRIVDHAAERQEALDRYAARGA
                     NP"
     gene            6096..6857
                     /locus_tag="PKU75_13190"
     CDS             6096..6857
                     /locus_tag="PKU75_13190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HPF81879.1"
                     /translation="MTGPAAAGHRRLRRGPVAELRAFLHAALVAPVTRDHAQTDAAFR
                     RRRVVAAVALLCGSAVLAWALRIPPGDPAFSWATFLLAGIWSAGALASGPLHLGRAHT
                     RAGGPDALPIVQSLALGGLLVVVFALGALVVAQVPALSAQVNAVLDHARVGSLGLIAA
                     IVAVNGIAEELYFRGALYAAIGRRYAVTITTAVYALTTAGTGNPMLVLAAALLGLLTG
                     LQRRVTGGVLGPILTHVIWSSSMLFLLPPILETLA"
     gene            6926..>7712
                     /locus_tag="PKU75_13195"
     CDS             6926..>7712
                     /locus_tag="PKU75_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPF81880.1"
                     /translation="MTGASGYIGGQLVPALLEDFTVRVLTRSPDGVERQAWRDEVDVV
                     VGDASRPEDLRRALDRVDVAYYLIHSMEADTTGFEERDRAAATLFAEEAERAGVQRLV
                     YLGGLHPRDERLSPHLRSRVEIGRIFLDSSVPAACLQAAVILGDGSASFEMLRYLTSR
                     LPAMVTPSWVDNPVQPIAAEDAIRCLRAAADLPSGVNRTFDIAGPDVLSYRQLMQTFA
                     EATGRRRRLILPLPVLTPGLASLWVGLITPVDAALARPLVGSLI"
BASE COUNT         1167 a         2697 c         2669 g         1179 t
ORIGIN      
        1 atcgccgtct tccgcgagcc cgtgacgccc actgagaagg atttcgaggc ccggctgtgg
       61 caggtcctgc aacagctgca cgacgaggac acgcacccgt gggctgacgg cgtcgccgca
      121 gacccggagg ccccgcaatt tgccttcagc cacgccgggc gcgcctactt catcgtggga
      181 ctgcaccccc gggcctcacg catcgcccgg cgagcgccgc tgcccacgct cgtcttcaac
      241 ctgcacgagc agttcgagaa gttgcgcgcc gaagggggtt tcgatcggat gcgcaccgcc
      301 attcgccgcc gcgacaccaa ggtgcaaggg tcggtcaacc cgatggccgc cgatcatggc
      361 gaagcatcgg aagcgcggca atactcagga cgccgcgttg agccgacgtg gcaggccccc
      421 ttctctccca aggagatcgg cgacgatcga tccgggtgac ccgctccggc gggggacacc
      481 gacgccaaca tctcaggcga cggggacttg ttgcgccgga cgagaccccg ggcatcgcgt
      541 cacggacgcg actccaggcg ggcgcgcagg accgagacgg cggtcacgat gtcgtgcccc
      601 aggggtgttg cggtctcgca gatgtcgtcc tggtatgcgc cgcccgcgaa gacgcgaatg
      661 gccgggacgc tcgcgagtgc cttggtcgtc cgccgggtgg tcgccacgtc gacgcgggtg
      721 ggcaccgcga tgacggccgc ggccgcacgt gtctggcgcg cgcattgcac ccaggcggcg
      781 gcgggcaggt cggcgcccag atagcggacc ggcagggcgt cccgggccgc cagcgcggcg
      841 aatgccaatg cccccaattc gtggtggctg ccgctcggca ggcccacaag caccggagca
      901 gcgcgtgcag tggcggtata gcggtgcaag cgggtggcca aagcccgatg gatagcgtgg
      961 gagatcaggt gctcggtcgc gatgcccacg gtgccatcga cccacccctc gccgacggca
     1021 gtcagggccg ggacgagcca gccggaaacg gcgaccggca atggtgcggc cagcccagca
     1081 gccacggcgg cggagatgac agccggatca gcggagcggg ccgccgcgat gagctccgag
     1141 atgggtggtg acggcgacgc tgccgcgaca tcctgtcgcc ttccggcgac gatccgcgcc
     1201 gcctcgctgg ccgggtggcc cacggcgacg agctcccgca tcacccgcag gtcctggatc
     1261 agtgactcgt cgtagaggcg gtagccggtg gggctgcgcg gggggcggac cacggcatac
     1321 cgccgctccc aggcacgcaa ggtagccggc gggatccccg tcgcctccgc gacggccttg
     1381 atcgtgtaca cgggccgacc ctagccatcc gccgtccgcg gggtcaggcg cagctgcagc
     1441 acgtcgaggt gcccggaccg gaagccggcc tccgaatagg cgagatagaa ctcccatacc
     1501 ttcaggaagc ggtcgtcgag acccgcggcg cggacggctc cggggttgtc caacaatgct
     1561 ttacgccact cccgcagcgt gcgtgcgtaa tcgtcgcgca gggagcgacg cgccgtcacc
     1621 cgcagcccgg cgctcgacgc atggtgagcg atcaccgcgt acgaggggat cagcccaccg
     1681 gggaagacgt gcttgtggat ccagccgtag gcgtgccggg agtcgagcat gcgggagtgc
     1741 gccatcgtga tggcttggat cgccacctgc ccgtcggggc gcaccgcgtc agccaggctc
     1801 cggaagtatc gcggccagaa ctcgtagccg acggcctcga tcatctcgac gctgacgacg
     1861 gcgtcgtagc gtcccgcgat atcgcggtaa tcgcgcaatt cgacgtcgac gacatcggcg
     1921 aagcccgcgg cggcgatgcg ctcgcgcgcg aggctctgtt gctcgtgaga gagcgtgatg
     1981 gtgtggacca gcgcgccccg ctctgccgcc tggatcgcca gttgtcccca tccagaaccg
     2041 atttcgagca cctctgtccc cgcgccgaca ttagccaggt cgaggatgcc ggcaaccttg
     2101 cgccgttgtg cggtggctag gccggccggt cccgccgggt cctcgggtgc gaaccaggcc
     2161 gccgaatagg tcatcgtctc gtccagaaac acggcgaagg cgtcattgga caggtcgtag
     2221 tggtgggaga tattggctcg ggctccggcg cggtcattgc gatggcgttc cggttgccgc
     2281 ggctcgacca atctccggaa tctcgtgagg accggtggca ccaggtcgcc aaggttcgcg
     2341 gcgaaccggg tgagcagatc cgcgaggtcg gtgcctgggc cgggtcccca ctccccggcg
     2401 acgaagcctt ccccgacacc gatcttgaag tcgatcccga tgcggtcgaa gatccgctcg
     2461 tcatgcacga gaaggtgcgg cgcgtcaccg cagtcgggtc ccatgccgct gccgtcgggc
     2521 agcgtgatgc gaacgcccgc gcgatcggcc atgcggcggg tcaggaggcg aacggcccgt
     2581 ccccgcacgc gagctcgccg cgggtcgtcg ggttgcgtcg ccgggcggga ccacgcccac
     2641 agtcgggcga tgggtgcggc cgtcatcgga agccttccgg ggggatgtgg gcgggacgga
     2701 tttggaccgg cagccggcgc agccagagcc agatgccgtg gatgcggatg cgggtcgaaa
     2761 cctggtaggt gatcagtggt gtggtggcca tggcggtgag caggcgggcc cgcgtggctg
     2821 gcacgcggtc acccaccaat gttgccgcga atcggcgaat gctgttttcc cgcaatgtta
     2881 tggacaggac aactcggtct cgctcaagtc gtgcgatgac ggcatactcc ccgctgacgg
     2941 gaaagaaggg cgagacgtag aactccttgg cgaccacggc tcgtccgtgc cggtcgggat
     3001 ggaggaggtg cgcatggcgc tcgccgtacg tgttgtggat ctccagcacc acccagcgca
     3061 cgcggccatc ggaagccacg ccgaaatagg tcgtcagggg attgaagaca tgcccggcaa
     3121 cacgcgcgtt cgtcatcatc aggacgcgct caccgtcccg cacggcctcc ccgcgcgtgc
     3181 ggagaaactc cccgagtgac tcgacgatgc tgtcggcctg gccgccgaag tgatcacgcg
     3241 gcaggatggc cgcgaaccag cgagcccggg ggatcgcggc gacgtcgacc agccacaggt
     3301 agccacgaaa gcgcacttgg tgccggatcg gcgagaagcg gtggtgggtg atgcgcacgc
     3361 gcacgagttg cgcgccggtg tcgcgcatca tgcccacctc gtcgctgcgg cgatgcgctc
     3421 cgcggcctcg agccccgacc gggcgccatc ctcatggaaa ccccagccca ggtgagcccc
     3481 ggcgaaggcg aggcgagggc cgcccagatc tcgcaatcgc gccgccgcag caacggactc
     3541 gcgagtgaaa acggggtgtc gatagcgcat ctgggcgaga taggtgaagg gatcgacgtc
     3601 gttcgcattg agcgagacga tgaactgagt agccccatcg aggccgtgca agcggttcat
     3661 ccagtagtcc acccgggccg gttgggtcag attggcacac gtggactgcc ggtagttcca
     3721 gctcgccctg gcccggcgcc gtcgcgggag gacgcgctcg tcgtggtgca gcacaacgtg
     3781 attgtccgag tatggaatcg cttccagcgc agcagcttcg gacgccgtcg cgtcggtcag
     3841 gatcgtgcgg gcgtcgtcgg cgtgcaccgc aaggaccagc ccatcggctg ccagcaccga
     3901 cccgtccgcc agccgcactt cgacctcccg gtcgagtctc ctcacggcga cggcgcccgc
     3961 gcccgtgcgg atgtcgggaa ggagcgcggc cagcgcctcg acgtagcgcg ccgatccgcc
     4021 gacgatcgtt cgccacgtcg gtgaaccggt cacgctgagc atgccgtgat tgtcgagaaa
     4081 ggcgaagagg tgatgcgcgg gatagtccaa cgcgacccgc tgtccggccg accagacgca
     4141 cgcgaccagc ggcccggcga agtggccgac aaagtatggg gaaaatccgt ggtcggccag
     4201 gaagccgccc cacgtcgtct cgcggtctcc cgacgcgagc acgtcccgag cggcccgatg
     4261 gaagcgtggc acgtcctgga ggagcgcgcg gaagcggcga tcggcgacgc gagcggggtc
     4321 ggcgaggatg cctccgagtc ccttgccgcc ggcatacgac aggccgcagg cgtcgcaggt
     4381 caccgacatg ctcatctccg tgggccgggt ggcgatcccg atgtcgcgca agaggctgag
     4441 aaatgtcgga taggtgcgct cgttgtgcac gatgaatccg gaatcgatgc gcagcgggcc
     4501 gtcggccgtc ggcacctcat gggtgtgggc gtgcccgccg agacgctcgt cggcctccac
     4561 gagcgtcacc cggtgggtgc gggcgagccg gtgggcggcg gtgagcccgg acacaccggc
     4621 accgacgacg atcacgctcg gccgccgatc cgcattgtcc acgtcgttca tgtccttcct
     4681 gacccaaaga cgtcgtccgt cgacgaggcg atttgtccaa ggtttgtcta aggttagggc
     4741 atgaccggtg tcgtgtggct acgcagggat ctgcgcgttc gcgaccaccc ggcgctctgc
     4801 gccgcggccg ccgaggggcc gatcgtcccg ttggtcgtgc tcgacccggt cctgacccag
     4861 gacagcccgc ggttcgaatc ctacctccat gccgtccgcg ccctgcgcga ggatctcgac
     4921 ggactcgtcg tgcgagtcgg tgacccgcga tacgtcgttc ctgcggtctg cgctgaggcc
     4981 cgcgcccgcg ccgtgcacgt ctccgccgag gtcacgccct acggccggca ccgggacgct
     5041 gccgtccggc tggccctggc gcgagccggg gtggagtgga tcgcgaccgg ttcgccgtat
     5101 gccgtgacgc ccggcagggt gcgcacgcga gccgggatgc ccttccacgt cttcacgccg
     5161 ttcgctcggg cctggcgcga cagccccgtc ccgagcccgc cggacccgcc ggcccgggtc
     5221 cggtataccc gcgtggtctc ggccgacctt cccgagaccg gcaccgcaac gcaccccgtt
     5281 cccgtgacgg aggcgcaggc gcgggctcgg tggcaggagt tcctcacgga tgagatcgcg
     5341 ggctacggcc gggatcgcga tcgccccgac cacgaggcga cctcacgcct gtcgaccgcc
     5401 ttgaagctcg gatccatcca tccccggacg ctgctgtccg acctgcggtc acgggccgcc
     5461 acccgcagcg acggcgtcga gcgattcatt accgaactgg cgtggcgcga gttctatgcc
     5521 gatgtgctgt ggcaccatcc gcggtccgcg tggcacgacc tcaaggacag ccttcacggc
     5581 atgcagtatg accatcccga gacgaatccc cttgccgccc aacatctctc agcttggcag
     5641 gcaggccgca ccggctatcc tttcgtcgat gccgggatgc gtcagctgcg cgcggagggg
     5701 tggatgcaca accgcgtccg catggtcacc gcctcgttcc tgaccaagga tctgcacatc
     5761 tggtggcccc acggcgcccg ccacttcctc gacctgctcc gcgatggcga tctggcttcc
     5821 aactcccacg ggtggcagtg ggtggccggt accgggactg acgcagcgcc ctacttccgc
     5881 atcttcaacc ccgtcaccca ggggctccgt ttcgatcccg acggggagta tgtccgccgc
     5941 tacgtgcctg agctggcaca cctgccgggg gcggcggcgc acgagccgtg gaagcacccc
     6001 ggcccgcacg tcgcaggcta cccgccacga atcgtcgacc atgcggccga gcgccaagag
     6061 gcgttggacc ggtatgccgc acgaggggcg aacccatgac ggggccggcc gctgccggcc
     6121 accgacggct ccggaggggg ccggtcgccg agttgcgcgc cttcctgcac gccgcgctcg
     6181 tcgcgccggt cacccgcgac cacgcgcaga ccgacgccgc cttccggcgc cgccgcgtgg
     6241 tcgccgcggt cgccctgctc tgcgggtcgg cggtgctcgc ctgggcgctg cggatccccc
     6301 ccggggaccc tgccttctcc tgggcaacct ttttgctcgc gggaatctgg agcgccgggg
     6361 cgttggcgtc cgggccgctc catctcggcc gcgcgcacac ccgcgccggc ggtcccgacg
     6421 cccttccgat cgtgcagagc ctggcgctgg gcgggctgct ggttgtggtc ttcgccctcg
     6481 gcgccctcgt ggtcgcgcag gtgcctgcgc tcagcgcgca ggtcaacgca gtcctcgatc
     6541 acgcccgagt cggctcgctc gggctgatcg ccgccatcgt cgctgtcaac ggcatcgccg
     6601 aagagttgta ctttcgcggc gcgttgtatg ccgccatcgg acggcgctat gccgtcacga
     6661 tcaccactgc cgtctatgcc ctgacgaccg cgggcaccgg caaccccatg ctcgtcctgg
     6721 ccgccgctct gctcgggttg ctcaccgggc tgcaacgccg ggtcaccggt ggagtgctcg
     6781 gccccatcct cacccacgtc atctggtcga gctcgatgct cttcctgcta cccccgatat
     6841 tggagacgct cgcatgagta cgacggattc ccctgccgca caatctactt cgccggcagg
     6901 gcccccgggg aaacgaacgg ttctggtgac cggtgcgtcc ggatacatcg gagggcagct
     6961 ggttcctgct ctcctcgagg acttcaccgt ccgggtcctg acccgttccc ccgacggcgt
     7021 cgaacgtcaa gcctggcgcg acgaggtgga cgtcgtggtg ggggatgcgt cacgaccgga
     7081 ggacctgcgc cgcgcgctgg accgagtcga cgtggcgtac tacctcatcc attcgatgga
     7141 ggcagacacc accggcttcg aggagcggga ccgtgcggcc gcgacgctct tcgccgagga
     7201 ggcggagcga gccggggtgc agcggctggt ctatctcgga ggactgcatc cgcgcgacga
     7261 gcggctctcc ccgcacttgc gatctcgcgt cgagatcggc cgtatcttcc tcgactcctc
     7321 cgtgcccgcc gcgtgcctgc aagcagccgt catcctcggg gacggatcgg cttccttcga
     7381 gatgctgcgc tacctcacca gccggctccc ggccatggtc acgccgtcgt gggtggacaa
     7441 ccctgtgcag cccatcgccg ccgaggacgc cattcgctgc ctacgggcgg cagcggacct
     7501 cccctccggc gtcaatcgca ccttcgatat cgccggaccg gacgtgttga gctaccgcca
     7561 gctcatgcaa accttcgccg aggccacggg tcggcgccgg cggctgatcc tgcccttgcc
     7621 cgtgctcacg ccggggctgg cgagcctgtg ggtcggtctg atcacgcccg tcgatgcggc
     7681 cctggcccgg cccctcgtcg gaagccttat cc
//
LOCUS       DAOPJG010000166         7690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_10152_length_7690_cov_8.136739, whole genome shotgun sequence.
ACCESSION   DAOPJG010000166 DAOPJG010000000
VERSION     DAOPJG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10152_length_7690_cov_8.136739"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKU75_13200"
     CDS             <1..206
                     /locus_tag="PKU75_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HPF81881.1"
                     /translation="YQLYFDERTLDGDLVRDFDGVEVVVDRMSAPYLDGATIDFADTI
                     EKQGFTIDNPNAGSSCACGDSFS"
     gene            453..1073
                     /locus_tag="PKU75_13205"
     CDS             453..1073
                     /locus_tag="PKU75_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81882.1"
                     /translation="MRSTLDKLISWLGLALAALLLIGGGLLLWGANFTKSNVTDQLKQ
                     QDITMPSGSAIEDPLIKPYLEKYAGQEMTNGDQAKAFADHYIKVHMDKSSGGRTYEDV
                     SGEFIALSKDANADKTKLEELGALRQSLFMGNTLRGLLLNAYAFGTMAKIMTIAGITA
                     LVGSLLMALLGLLGLRHAKQVRDEESPLVRRDEATRRDGGATAATV"
     gene            1174..2160
                     /locus_tag="PKU75_13210"
     CDS             1174..2160
                     /locus_tag="PKU75_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPF81883.1"
                     /translation="MKILVTGSIASDHLMTFDGRFADSLVVEQLDKISLSFLANDLEI
                     RRGGIAANIAFGMANLGMSPVLVGAVGEDFDAYRSWLERHGVDCESILVSDTKHTARF
                     VCTTDKDMAQIATFYAGAMSEARRIELGPIHKRVGGADYVLIGANDPEAMLRHTRECR
                     KRGLKFIADPSQQLAFSNGPEIKELIDGATYLITNEYEARLTEQKTGWSATDIAERVT
                     HRVTTHGKDGAIIESPGEDPIHVGVAKVARLADPTGVGDAFRAGFLAGLSVGLPHRRC
                     AEMGSVLAAHVIETVGTQEYELSKRTFLARLADAYGEDSATEIAPHLKCPRP"
     gene            2145..2867
                     /gene="aat"
                     /locus_tag="PKU75_13215"
     CDS             2145..2867
                     /gene="aat"
                     /locus_tag="PKU75_13215"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPF81884.1"
                     /translation="MPTPLAPRPIEPPPTGWSFRRLIAQAPPSDLIGVGGDLEPGTLL
                     AAYREGVFPMGVDLDDEHVLGWWSPTERGVLRPANLHVSRSLRRSARTMQLSVDADFA
                     GVVAGCADPQRSGAWITPEIAAAYQRLHELGWAHSIEVWREGELAGGLYGVAVGGLFA
                     GESKFHRVTDASKLALAGLVDVMRSGAGPALIDVQWRTDHLATLGIEVMSRPDYLAAL
                     PVLITAPGPDWAAWRGRRWARR"
     gene            complement(2864..3145)
                     /locus_tag="PKU75_13220"
     CDS             complement(2864..3145)
                     /locus_tag="PKU75_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPF81885.1"
                     /translation="MTEHSEPGPGDRGAGGLDDGDAVIVDARETRCPMPVIMAAKAAQ
                     DLPDGARLVVLATDPAAAADLPAWCRLRGHTLLDLHLGDEVRADIRLGT"
     gene            complement(3145..4320)
                     /locus_tag="PKU75_13225"
     CDS             complement(3145..4320)
                     /locus_tag="PKU75_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPF81886.1"
                     /translation="MAAFPAQRAFLDAASGPLHPLAAQTMTAAVDAGWADPRRLYREG
                     RQAAALLNQARETIAADLGVRPSELSFHFGGGPAALTAALEGLAWPRRRAGSRLVASA
                     VEHSNVLIAGRYAVAQSGDPLRFAEIGVDRAGRVDLDAFAAALTDPTTAVAALQHANG
                     EVGTMQPVDAAYRACAERGIPLLVDGQASVGRVRAPASYAALVADAATVAGPPLGLLV
                     VPERTAFRRPGPPREAEFGRADAPVWVPLALAAAEAWQQAAAVRDDDAGSARALTDRI
                     RAAAAAIPDVEVAGDDGERLPHIVTCSALYVDGETLVGELDRRDIAVASGSACTASTL
                     EPSHVLAAMGLLTHGNIRITVPWRATAPDRDSGVDRLITALPLVIGEVRARLGAGGL"
     gene            4451..5338
                     /gene="coxB"
                     /locus_tag="PKU75_13230"
     CDS             4451..5338
                     /gene="coxB"
                     /locus_tag="PKU75_13230"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HPF81887.1"
                     /translation="MRLSDTVTRRRSRAGLGLALTAAVLSLGGCGLAGAQGRVQDGYL
                     PQGATEGAERVRSLWIGSWIAALAVGVLVWGLTAWCVVAYKRKKDDPPLPPQLKYNIP
                     LEILYTVVPIMMIGVLFYYTDRDERELTDTKQTPDVTIQVIGKQWSWDFNYLEGNAHE
                     SGTQAQLTGKPGVEETLPSLYVPVGKRIEFQLVANDVIHSFWVPAFLQKLDMIPGRVN
                     TLQVVPTREGTFAGKCAELCGAYHSEMLFNLKVVSQAEYDKHINDLKAQGNEGLLPAS
                     VLREPIIEQDKDKLTTGSN"
     gene            5368..7053
                     /gene="ctaD"
                     /locus_tag="PKU75_13235"
     CDS             5368..7053
                     /gene="ctaD"
                     /locus_tag="PKU75_13235"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HPF81888.1"
                     /translation="MLRTAERVEHKRLAPGTTFVKWVTTTDHKVIGNLYFIASFAWFL
                     IGGLMALIIRLELFEPGIQIVDDKQQFNQLFTMHGTIMLLLFATPLFAGFANALVPLQ
                     IGAPDVAFPRLNMFAFWLYFFGGLIAAFGFLTPMGAAAFGWFAYAPLSNSVFSPGVGG
                     DLWVFGLAMGGFGTILGGVNFITTIVCMRAPGMTMFRMPIFTWTILITSLLIILIFPP
                     LAAALFALGADRRFGATIFDPANGGPILWQHLFWFFGHPEVYVIALPFFGIISEVLPV
                     FSRKPIFGYKTLIFATIAIAALSVSVWAHHMYVTGQVLLPFFAIMTMAIAVPTGVKFF
                     NWIGTMWGGHLVFSTPMIWAIGFLVTFLFGGLTGVILASPALDFSLSDSYFVVAHFHY
                     VVFGTVVFAMFAGFYFWWPKFTGRMLDETLGKIHFVLLFIGFHATFLIQHWVGVDGMP
                     RRYADYLPEDGFTLANQVSTVGAFILGASMLPFMYNVWKTWKYAPLVDTDDPWGYGAS
                     LEWATSCPPPRHNFNSIPRIRSERPAFDLNHPEGAPASLRRANAADALTAGKA"
     gene            7061..7462
                     /locus_tag="PKU75_13240"
     CDS             7061..7462
                     /locus_tag="PKU75_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HPF81889.1"
                     /translation="MKVEAIVFLVVGIFATVMGLIYGIVTDWQELVGAPALFLSGGLG
                     FMVAFYFWLTGKRIGYRPDDNPEGEIREFEGEYGSFSPYSWWPLWLGLTASLMFMGLA
                     VGWWMFAIGAFFGLFALVGWTFEYFKGAHAN"
     gene            complement(7481..>7690)
                     /locus_tag="PKU75_13245"
     CDS             complement(7481..>7690)
                     /locus_tag="PKU75_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81890.1"
                     /translation="SPRGAQWAREAAVRVLQIAGAAPLAETLGVASSYAAFGAGDALL
                     DPAVDERLAAVIGQLLDAARTPAAA"
BASE COUNT         1150 a         2769 c         2507 g         1264 t
ORIGIN      
        1 tctaccagct ctacttcgac gagcgcaccc tcgacggtga cctcgtgcgc gacttcgacg
       61 gtgtcgaggt ggtcgtcgac cggatgagcg ccccctacct ggacggcgcg accatcgact
      121 tcgccgacac catcgagaag cagggcttca ccatcgacaa ccccaacgcc ggcagctcct
      181 gcgcctgcgg agacagcttc agctgaccca ccccaagacc gacggcccgg gcgctgaccc
      241 ctgcgcgccc gggccgtcgc ctgtctgcgc tgcgcgtctg acacgctgtc gtgctcgccg
      301 tcgcgacacg ccgtcgagct gggtacccac tcgtagttga atcataaatt gtatcggcgt
      361 aggctgtcac cagccggtca tcgaagccgg cccggggctg cggagcccga cccgagtggc
      421 tctccccgga gatggaggag accccccact caatgcgtag cactctcgac aaactcatct
      481 cgtggctcgg cctcgcgctg gccgcgttgc tcctgatcgg cggcggcctg ctgctgtggg
      541 gagccaactt caccaagtcc aatgtgaccg accagctcaa gcagcaggac atcaccatgc
      601 cgtccgggtc ggccatcgag gacccgctca tcaagccgta cctggagaag tacgccggcc
      661 aggagatgac caatggcgac caggccaagg ccttcgccga tcactacatc aaggtccaca
      721 tggacaagtc gtccggcggg cgtacctacg aagatgtctc cggcgagttt atcgccctga
      781 gcaaggacgc caacgccgac aagaccaagc tggaggagct cggcgcgctg cgtcagagcc
      841 tgttcatggg caacaccctg cgcgggctgc tgctgaacgc ctacgccttc ggcaccatgg
      901 ccaagatcat gaccatcgcc gggatcaccg ccctcgtggg ttcactgctc atggccctgc
      961 tcgggctcct cggcctgcgg catgccaagc aggtccgtga cgaggagagc cccctcgtcc
     1021 gtcgggacga ggcgacccgc cgcgacggcg gcgccacggc ggcgaccgtc tgaggggtag
     1081 ctgccccaac cgcatacccg caccgcgccg gtcctcgttc gaggaccggc gcgttcgcgt
     1141 cagcggcccg gaggaggcag ataatgtcgg cccgtgaaga tcctcgtgac cggttccatc
     1201 gccagcgacc acctcatgac cttcgacggc cggttcgccg actctctcgt cgtcgagcag
     1261 ctggacaaga tctccctgtc cttcctggcc aacgatctgg agatccggcg gggcgggatc
     1321 gccgccaata tcgccttcgg gatggccaac ctcggcatgt cgccggtgct ggtcggcgcg
     1381 gtgggggagg acttcgacgc ctaccgcagc tggctcgagc ggcacggcgt cgactgcgaa
     1441 tcgatcctgg tctccgacac caagcacacc gcccgcttcg tgtgcaccac cgacaaggac
     1501 atggcccaga tcgcgacctt ctatgccggg gcgatgagcg aggcccggcg catcgaactc
     1561 ggcccgatac acaagcgcgt cggcggcgcc gactacgtgc tcatcggggc caacgacccc
     1621 gaggcgatgc tgcggcatac ccgcgagtgc cgcaaacgcg gcctgaagtt catcgccgac
     1681 ccctcccagc agctcgcgtt cagcaacggc ccggagatca aggaactcat cgacggcgcg
     1741 acctacctga tcaccaacga atacgaagcg cgcctgaccg aacagaagac cggctggagc
     1801 gcgacggaca tcgccgagcg cgtcacacac cgcgtcacca cccacggcaa ggacggcgcg
     1861 atcatcgaat ccccgggcga ggatccgatc cacgtcggcg tcgccaaggt ggcccggctg
     1921 gccgacccca ccggtgtggg ggacgccttc cgcgccggtt tcctcgcggg gctttccgtc
     1981 gggctgccgc accgccgctg cgccgagatg ggttcggtcc tggccgccca cgtcatcgag
     2041 accgtcggca cccaggagta cgagctgtcc aagcgcacct tcctcgcgcg cctggccgac
     2101 gcctacggcg aggactccgc gaccgagatc gccccgcacc tgaagtgccc acgcccctag
     2161 cgcctcggcc catcgaaccc ccgcccaccg gctggtcctt ccggcggctg atcgcgcagg
     2221 cgccgccgag cgatctgatc ggtgtgggcg gggacctgga gccgggcacc ttgctcgcgg
     2281 cctatcggga gggtgtcttc ccgatgggcg tcgacctcga cgacgagcat gtcctcgggt
     2341 ggtggtcacc gacggagcgg ggggtgctgc gtccggcaaa cctgcacgtc agccggtccc
     2401 tgcggcgctc ggcccgcacc atgcaactca gcgtggacgc cgacttcgcc ggggtggtcg
     2461 ccggctgcgc cgacccgcag cggtccggcg cgtggatcac gccggagatc gccgcggcat
     2521 accagcggct gcacgaactc gggtgggcgc actcgatcga ggtgtggcgc gagggggagc
     2581 tggccggcgg gctgtacggt gtggccgtcg gcgggctctt cgccggcgag tcgaagttcc
     2641 accgggtcac cgacgcctcc aagctggcgc tggcgggtct ggtcgacgtg atgcgctccg
     2701 gcgccggtcc cgccctgatc gacgtccaat ggcgcaccga ccacttggcc accctcggca
     2761 tcgaggtcat gtcgcggccg gactatctcg ccgctctccc ggttctgatc accgcccccg
     2821 gccccgattg ggccgcctgg cgtggccgcc gctgggcgag gcgctaggtc ccgaggcgga
     2881 tgtcggcgcg cacctcgtcg ccgaggtgca ggtcgagcag ggtatggccg cgcagccggc
     2941 accacgcagg caggtcggct gccgcggccg gatcggtggc gaggaccacg agccgggcgc
     3001 cgtcgggcag atcctgggcc gccttggccg ccatgatcac cggcatcggg cagcgagtct
     3061 cccgagcgtc cacgatcacc gcatcgccgt catcgaggcc gccggcgccg cggtcgccgg
     3121 ggccgggctc cgagtgctcg gtcatcacaa tcctcctgct ccgagtcgcg cgcgcacctc
     3181 accgatcacc agcggcagcg ccgtgatcag gcggtccacg ccggaatccc ggtccggtgc
     3241 ggtggcacgc cagggcacgg tgatgcggat gttgccgtgc gtgagcagcc ccatcgccgc
     3301 cagcacatgg ctcggctcga gcgtcgaggc ggtgcacgcc gagccggagg ccacggcgat
     3361 atcgcgccgg tcgagctcgc cgaccagggt ctcgccgtcg acgtacaaag cgctgcaggt
     3421 cacgatgtgg gggagacgtt cgccgtcgtc gccggccacc tcgacgtccg gtatggccgc
     3481 ggcggcggcc cggatccgat ccgtcagggc gcgtgccgac ccggcgtcgt cgtcgcgcac
     3541 cgccgccgcc tgctgccacg cctcggccgc cgccagggcc agcggcaccc acacgggggc
     3601 gtcggcgcgg ccgaactcgg cctcccgcgg cggtcccggt cgccggaacg ccgtgcgttc
     3661 cggcacgacg agcaggccga gcggcggtcc ggcgaccgtc gccgcgtcgg cgaccagggc
     3721 ggcatacgac gcaggggcgc ggacccgccc gacgctcgcc tgcccgtcga cgaggagagg
     3781 gatcccgcgc tccgcgcagg cacggtatgc cgcgtccacc ggctgcatcg tgcccacctc
     3841 gccgttggcg tgttggaggg ccgcgacggc cgtggtgggg tcggtcagtg cggcggcgaa
     3901 cgcgtcgagg tcgacccggc ccgcccggtc cacgccgatc tcggcgaacc ggagcggatc
     3961 accggactgg gccacggcat accgtccggc gatcagcacg ttcgagtgct cgacggcgct
     4021 cgcaaccagc cggctgcccg cgcgccggcg cggccaggcc aggccttcca gcgccgccgt
     4081 cagcgcagcc ggcccgccgc cgaagtgaaa actcagttcg ctggggcgaa cgccgaggtc
     4141 cgcggcgatg gtctcacgtg cctgatttag cagcgcggcc gcctggcgtc cttcgcggta
     4201 caggcggcgc gggtcggccc agccggcgtc cacggcggcc gtcatcgtct gcgcggcgag
     4261 ggggtgcagc ggcccgcttg ccgcgtcgag aaaggcccgc tgagcgggga aagcggccac
     4321 gcttcgataa cttagccgac cccttcgttg cgcggctttg agcctgcggt acctttactg
     4381 ccaagcacgt cgctgccagc gggtgcccgc tgcacaaaag tgaccgcatg tcgatccgaa
     4441 gaggtgcccg ttgcgactgt ctgacaccgt cacccgccga cgctcccgcg ccggactcgg
     4501 cctggcgttg acggctgccg tcctcagcct tgggggatgc ggcctggccg gcgcccaggg
     4561 ccgggtccag gacggctatc ttccgcaggg cgccaccgag ggcgccgagc gcgtccgcag
     4621 cctctggatc ggttcgtgga tcgccgcgct ggccgtcggc gtcctggtgt gggggctgac
     4681 cgcgtggtgc gtcgtggcct acaagcgcaa gaaggacgac ccgccccttc cgccgcagct
     4741 gaagtacaac atcccgctgg agatcctcta cacggtcgtc ccgatcatga tgatcggcgt
     4801 gctgttctac tacaccgacc gcgacgagcg ggagctgacg gacaccaagc agacgccgga
     4861 cgtcaccatc caggtcatcg gcaagcagtg gagctgggac ttcaactacc tcgagggcaa
     4921 tgcccacgag tccggcaccc aggcccagtt gacgggcaag cccggggtcg aggagacgct
     4981 gccttcgctc tatgtgcccg tgggcaagcg catcgagttc cagctcgtcg ccaatgacgt
     5041 catccactcc ttctgggtgc cggccttcct gcagaagctc gacatgatcc ccggtcgcgt
     5101 caacaccttg caggtcgtgc cgacccgtga gggcaccttc gccggcaagt gcgccgagtt
     5161 gtgcggcgcc taccactcgg agatgctctt caacctcaag gtcgtctcgc aggccgaata
     5221 cgacaagcac atcaacgatc tgaaggccca gggcaacgag ggtctgctgc cggcgagcgt
     5281 gctgcgtgag ccgatcatcg agcaggacaa ggacaagctc acgacgggga gcaactgacc
     5341 atgagcagca tcacgaacga cgggacgatg ctccgcacgg ccgagcgcgt cgagcacaag
     5401 cgcctcgccc cgggcacgac cttcgtcaag tgggtcacga ccaccgacca caaggtcatc
     5461 ggcaatctct acttcatcgc ctccttcgcc tggttcctca tcggcggcct gatggcgttg
     5521 atcatccgcc tggagctctt cgagccgggc atccagatcg tcgatgacaa gcagcagttc
     5581 aaccagctgt tcacgatgca cggcacgatc atgttgttgc tcttcgcgac acccctgttc
     5641 gccggcttcg ccaacgcact ggtgccgctg cagatcggcg cccccgatgt ggcgttcccg
     5701 cgcctgaaca tgttcgcctt ctggctctac ttcttcggcg gcctgatcgc ggccttcggc
     5761 ttcctcaccc cgatgggtgc ggctgctttc ggctggttcg cctacgcacc gttgtcgaac
     5821 tccgtcttca gccccggcgt cggcggtgac ctgtgggtct tcggcctggc catgggtggt
     5881 ttcggcacca tcctgggtgg cgtcaacttc atcacgacga tcgtgtgcat gcgtgccccc
     5941 ggcatgacga tgttccggat gcccatcttc acgtggacca tcctgatcac ctcgctgctg
     6001 atcatcctca tcttcccgcc gctggccgct gcgctcttcg cgctcggtgc cgaccgtcgc
     6061 ttcggggcca cgatcttcga ccccgccaac gggggcccga tcctgtggca gcacctgttc
     6121 tggttctttg gccaccccga ggtctacgtc atcgcgctgc cgttcttcgg gatcatctcc
     6181 gaggtgctgc cggtgttctc gcgcaagccg atcttcgggt acaagaccct gatcttcgcc
     6241 accatcgcca tcgccgcctt gtcggtcagc gtctgggcgc accacatgta cgtcaccggc
     6301 caagtgctgc tgcccttctt cgccatcatg acgatggcga tcgcagtgcc gaccggcgtg
     6361 aagttcttca actggatcgg cacgatgtgg ggtggccacc tggtcttctc cacgccgatg
     6421 atctgggcga tcggcttcct cgtgaccttc ctcttcggtg gcctgacggg ggtcatcctg
     6481 gccagcccgg cgctcgactt cagcctgtcc gactcctact tcgtggtcgc gcacttccac
     6541 tacgtcgtct tcggcaccgt cgtcttcgcc atgttcgccg gcttctattt ctggtggccg
     6601 aagttcaccg gtcgcatgct cgacgaaacg ctcggcaaga ttcacttcgt gctgctgttc
     6661 atcggcttcc acgccacctt cctcatccag cactgggtgg gcgtggacgg tatgccgcgc
     6721 cgttacgccg actacctccc cgaggacggc ttcacgctcg ccaaccaggt ctccaccgtc
     6781 ggcgcgttca tcctgggcgc ctcgatgctg ccgttcatgt acaacgtgtg gaagacctgg
     6841 aagtacgccc cgcttgtcga caccgacgac ccgtggggct acggcgcctc actggagtgg
     6901 gcgacgtcct gcccgccgcc gcggcacaac ttcaactcca tcccgcgcat ccgctccgag
     6961 cgtccggcct tcgacctgaa ccaccccgaa ggcgcccccg cttcgttgcg ccgcgccaac
     7021 gcggccgacg ccctgaccgc cggaaaggcc tgatgcctgt atgaaggtcg aagccatcgt
     7081 cttcttggtc gtcgggatct ttgccaccgt catgggcctg atctacggca tcgtcaccga
     7141 ctggcaggaa ctcgtcgggg cccccgcgct gttcctcagc ggcggcctgg gcttcatggt
     7201 cgccttctac ttctggctga ccggcaagcg catcggttac cggcccgacg acaaccccga
     7261 gggtgagatc cgggagttcg agggcgagta cggctcgttc tcgccctact cctggtggcc
     7321 gctctggctc ggcctgaccg catccttgat gttcatgggc ctggccgtcg gctggtggat
     7381 gttcgccatc ggcgccttct tcggcctttt cgccctcgtc ggctggacct tcgagtactt
     7441 caagggcgcc cacgccaact gagctgggtc ctggagggcg tcaagcggcg gcgggggtgc
     7501 gtgccgcgtc gaggagttgg ccgatgacgg cggcgagtcg ctcgtccacg gcggggtcca
     7561 ggagcgcgtc gccggcgccg aacgcggcat aggacgacgc aacaccgagc gtctcggcca
     7621 ggggagcggc gccggcgatc tgcaggacgc gcacggcggc ctctcgggcc cactgcgcgc
     7681 cgcgcgggga
//
LOCUS       DAOPJG010000167         7675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_10179_length_7675_cov_7.693963, whole genome shotgun sequence.
ACCESSION   DAOPJG010000167 DAOPJG010000000
VERSION     DAOPJG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10179_length_7675_cov_7.693963"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..671
                     /locus_tag="PKU75_13250"
     CDS             3..671
                     /locus_tag="PKU75_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81891.1"
                     /translation="MTAQPLGGRSPLGPGAFKLNYPMSLGVYDSYHEAQKAVDYLSDN
                     GFPVENVLIVGTDLKQVERVTGRLTRGRVAMGGALSGAWLGAFVGLIFSMFEDGQTLV
                     RILSTVIFGALFGLVWSMVGYALTKGQRDFTSVSQVVATRYEVMTEHKYVQQGRDLIA
                     QMDPMKAAQAQVAEAQARAYAEAQAAAQTPPTTQAQSDAQARPPAQFPASPPQQPPPP
                     TSGF"
     gene            707..1468
                     /locus_tag="PKU75_13255"
     CDS             707..1468
                     /locus_tag="PKU75_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPF81892.1"
                     /translation="MTPPGPPSAPPSGPPELTVLDEAFDCVLCVVAHPDDIEYGTSAA
                     VARWTGAGKTVTYWLATRGEAGIDNLHPDQAGPVREAEQRAAGRAVGVEIVEFGSYRD
                     GIVEYGLDLRRDIAREIRRRRPGLVVTGDYHDRFVAGMLNQADHRAVGQACADAVADA
                     GNRWIFPELLDEGLEPHHVPLLAFAGTATPTHYVDVTATFTAAVASLEEHDTYNRGLP
                     EQFPKPAQLLGMVLGSVKPAPDVDYALLLDVVERR"
     gene            complement(1492..1812)
                     /locus_tag="PKU75_13260"
     CDS             complement(1492..1812)
                     /locus_tag="PKU75_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81893.1"
                     /translation="MSLARLSGRTDADGRCVPLAQQDPATWNRALIDRGRAHLKAAHD
                     ARQLGRFHLEAAIQAVHCSRVDGAEPDPRLLVDLHRGLVAVSPTLGAIAARQHLLSQR
                     ETWT"
     gene            complement(1885..2256)
                     /locus_tag="PKU75_13265"
     CDS             complement(1885..2256)
                     /locus_tag="PKU75_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPF81894.1"
                     /translation="MRYSLLLHYPEPTEGQIDPEMIAQAQRAFQAYAAALDAAGVLIA
                     AEVLQPSAATTTVTRRTGDLSVQDGPFAATVEALAGVFVLDVPHLDAAIGWAEKCPAA
                     QWGVIEVRPAGTSYVDGAWTH"
     gene            complement(2468..3316)
                     /locus_tag="PKU75_13270"
     CDS             complement(2468..3316)
                     /locus_tag="PKU75_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPF81895.1"
                     /translation="MKDGRPRSMFSIISMILLCIIWTLPTLGLLVTSFRSRDDAQSNG
                     WWNALFNPFGTGWTFENYRKVIADADLGNAFLNGIVVALPATILPIMFAAFAAYAFTF
                     LDFPGKDLLFIAIVAVMVVPIQVAFQPMLNLLGPNGLGISGQYIAVWLLHTGFGMPLA
                     IYTLRNYMSTLPYSVVESAKIDGATHYQIFWKLVAPMAMPAMAAFATLQFLWVWNDLL
                     IAKLFLNSGNSTVIVKLQGLLGTQGQGQELLTAGAFISMVLPMIVFFALQRFFVRGLT
                     SGAVKG"
     gene            complement(3379..4527)
                     /locus_tag="PKU75_13275"
     CDS             complement(3379..4527)
                     /locus_tag="PKU75_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010532722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF81896.1"
                     /translation="MSTATDTVVAPQRRDPDDINRANVPKIVIGLFGLILTAWALGNL
                     FLAFGYYPQWFLDNKILIAALALIGGVGGAALLFWFLNMFIEGLPNRLSQGIMPYAFL
                     LPGFGLIALMLIYPTIQTINYSFANANSTEYVGLANYKDIFGSKDFWNATLNNLLWLA
                     IVPAITVAIGIIVAVLADKLSAQGEKVSKSIIFLPMAISFVGASAIWNLVYAYSDKSQ
                     SQVGLLNAIVTKLGMDPKTWLQISDGKLNSLLMMVILIWLQVGFSMILLSSAIKGVPD
                     DTIEAARIDGASELQIFFKVIIPQIRGTIVTVFITVVILVMKVFDIVYVLTNGRDNSD
                     VIANLFFNEMFANSQAGRASAIVVVLLIAVLPILWYQVKTFREEQMNR"
     gene            complement(4527..5882)
                     /locus_tag="PKU75_13280"
     CDS             complement(4527..5882)
                     /locus_tag="PKU75_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPF81897.1"
                     /translation="MAQVHRRRQLVAMGGAILTTTLLSACLQNPNASDGGGAAGLNGY
                     VDNTQKDGDKVVTILGAFGGDEKKAFEASLAEFEKSSGIDIQYTDDNNFTTIVKTRVT
                     AGDAPDIALFPQPGGLIELAEAGKIQPIDSVLDYDTLATTLVPGFLESTRYKGRSYGA
                     PMRMAVKSIVWYPKPAWTEAGGKTEFTSLQDLAKNTDEIKGKGTAPWCMGWGSDAATG
                     WVGTDWIEEMVLRVSGPDVYDQWVSHKIPFNDPQILKAFAEFGKLAKGDGNVFGGAAN
                     IINTKFGDAMTPAFQDPPKCYLMRQGNFATGFLPKDVQADLDNKVGIFVFPPLDGGYG
                     GQPILGGGDLAAQFNGNDPDTIEVMKFLTSDKFGGPWAKVGGWLSPHKTFDATNYPNE
                     LTKQMAEIVSSADVFRFDASDLMPKEVGSGTFWTEMVKWMNGASDEETATAIENSWPK
                     S"
     gene            complement(6141..7628)
                     /locus_tag="PKU75_13285"
     CDS             complement(6141..7628)
                     /locus_tag="PKU75_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPF81898.1"
                     /translation="MSEEQKTADNRARQTTEEFRAFVQTGWAPRADARPRRSPAAPYA
                     AARRATLSAAYPGERLVIPAGGLKVRSNDTDYVFRPHTAFAYLTGLGADREPDAVLVL
                     EPTADGHEAILFFRPLAARDSDEFFGDARYGEFWVGPRPSIEDVEAELDLSGRHIDDF
                     LDAVTKDAAEMIVRVVRDADSALTAQLDERRVSEGREQEQLQEADDELAHTISTSRMV
                     KDAFEIEQMRAAIAATKVGFEAVITDLPHLVAKGRGERWVEGVFGLHARHQGNGVGYD
                     SICAAGDHANTLHWIKNTGDVTEEDLILLDAGVEVDALYTADITRTLPVSGTFTEDQR
                     EVYDAVCAAQAAGIAAVKPGNKFSDVHAAAIRVIAEHLHAWGLLPEGIGVEETLDKDH
                     GQYHRRWMVHGTSHHLGLDVHDCSLATREEYMDAELRPGMILTVEPGLYFKADDDLAP
                     ERFRGIGVRIEDDVLVTEDGCENLSAGMPRSSAEVEAWIHHIQSR"
BASE COUNT         1255 a         2430 c         2681 g         1309 t
ORIGIN      
        1 gtatgacagc gcaaccgctt ggtggccgca gcccgctcgg tccgggcgcc ttcaagctga
       61 actaccccat gtcgctcggt gtctatgaca gctatcacga ggcgcagaag gccgtggact
      121 acctgtccga caacggcttt cccgtcgaga acgtgctgat cgtcggcacc gacctgaagc
      181 aggtcgagcg ggtcaccggg cgcctgaccc gcgggcgcgt ggccatggga ggggcgctct
      241 ccggtgcctg gctgggtgcc ttcgtcggtc tgatcttctc gatgttcgag gacggtcaga
      301 cgctcgtgcg gatcctctcg accgtgatct tcggtgccct cttcgggctg gtgtggtcga
      361 tggtgggcta cgcactcacc aagggccagc gcgacttcac ctccgtcagc caggtcgtcg
      421 ctacgcggta tgaggtgatg accgagcaca agtacgtcca gcagggccgg gacctgatcg
      481 cccagatgga ccccatgaag gccgcccagg cgcaggtcgc cgaggcacag gcccgggcgt
      541 atgccgaggc ccaggcagcc gcgcaaaccc cgcccacgac acaggcgcaa tcagacgcgc
      601 aggcccgacc cccggcgcag tttcccgcat cgcccccgca gcaaccgccg ccacctactt
      661 ccggattctg agcgacgggg gccgatcgag gcgtagcctc ggcgccatga cacctccggg
      721 accaccctca gcgcctcctt ccgggccgcc ggagctgacc gtcctcgacg aggccttcga
      781 ctgcgtcctg tgcgtcgtgg cgcatcccga cgacatcgag tacggcacct cggccgccgt
      841 ggccaggtgg accggtgccg gcaagacggt gacgtactgg ctcgcgaccc gcggggaggc
      901 gggcatcgac aacctgcatc ccgaccaggc cggcccggtg cgggaggcgg agcaacgcgc
      961 agccggtcgg gccgtcggcg tcgagatcgt cgagttcggt tcctatcgag acggcatcgt
     1021 ggagtacggc ctggacctgc gccgcgacat cgcccgcgag atccggcgcc gcaggccggg
     1081 tctcgtcgtc accggcgact accacgatcg gttcgtcgcg ggcatgctca accaggccga
     1141 ccaccgcgcc gtgggtcagg cgtgtgccga cgcagtcgcc gacgccggca accggtggat
     1201 cttcccggaa ctgctcgacg agggcctcga gccacaccac gtgccgctgc tggccttcgc
     1261 cggcaccgcc accccgaccc actatgtcga cgtcaccgcg acgttcaccg ccgcggtggc
     1321 gtcgctcgag gagcacgaca cgtacaaccg cggcctcccg gaacagttcc ccaagcccgc
     1381 ccagttgctc gggatggtcc tgggatcggt gaagccggca ccggacgtcg actacgcgct
     1441 cctgctcgac gtggtcgaac gccgctaggg gggccgccgg tgtgggtcgc gtcaggtcca
     1501 cgtctcgcgc tgtgacagca ggtgctgtcg cgcggcgatg gccccgaggg tgggggagac
     1561 ggcgacgaga ccacgatgga ggtccacgag caggcgtgga tccggttcgg ccccgtcgac
     1621 gcgggagcaa tgtacggcct ggatggcggc ctcgagatgg aagcgcccga gctgtcgcgc
     1681 atcatgtgcc gccttgaggt gggctcgacc gcggtcgatg agggctcggt tccacgtcgc
     1741 tggatcctgt tgcgccaacg ggacgcatcg gccgtcggcg tcggtgcggc cggaaaggcg
     1801 ggcgagcgac atgctgacca gcgcggtgag gccgcggacc tagggctcgc ccgcaccggc
     1861 tccggtgtcg tcaccgcatg gctcctagtg ggtccaggcg ccgtcgacat aggacgtgcc
     1921 ggccggccgg acctcgatga cgccccactg ggcggcgggg cacttctcgg cccacccgat
     1981 ggcagcgtcg aggtggggta cgtcgaggac gaacaccccg gcgagcgcct cgacggtggc
     2041 ggcgaaggga ccgtcctgca cactgaggtc gccggtgcgc cgggtgacgg tcgtggtcgc
     2101 ggcgctcggt tggaggacct cggcggcgat caggacacca gcggcgtcga gggcggcggc
     2161 atacgcctgg aaggcccgct gggcctgcgc gatcatctcg gggtcgatct ggccttcggt
     2221 cggctcgggg tagtgcagca gcagggagta acgcattgtc agatcctctc ggttcgcccg
     2281 gctccgtggt ggccggatac cccatgacga tcgaaggggg ccggattcgt cacccccttt
     2341 gcgtccgcgg agttggtcgc tatagcaacc aactccgcgg acgtaagccc ggggagggcg
     2401 gggtcatggg cacgcgaaag ggcccgcccg gagggagggc gggcccaatc gttggcgcgc
     2461 ggtggcgtca gcccttgacc gcgcccgagg tgaggccgcg gacgaagaag cgctgcaggg
     2521 cgaagaagac gatcatcggc agcaccatcg agatgaaggc gcccgcggtg agcaactcct
     2581 ggccctgacc ctgggtgccg agcaggccct gcaacttcac gatgaccgtg gagttgccgg
     2641 agttcaggaa cagcttggcg atcagcaggt cgttccacac ccagaggaac tgcagcgtgg
     2701 cgaaggcggc catggcgggc atggccatcg gggcgaccag cttccagaag atctggtagt
     2761 gggtggcgcc gtcgatcttc gccgactcga ccacgctgta gggcagcgtg gacatgtagt
     2821 tgcgcagggt gtagatggcc agcggcatac cgaatccggt gtgcaacaac cacaccgcga
     2881 tgtattggcc ggagatgccg agaccgttgg ggccgagcag gttgagcatc ggctggaagg
     2941 ccacctggat cgggacgacc atcaccgcga cgatggcgat gaagagcagg tccttgcccg
     3001 ggaagtccag gaaggtgaac gcgtatgccg cgaaggcggc gaacatgatc ggcaggatgg
     3061 tcgccggcag ggcgacgacg atcccgttga ggaaggcatt gccgaggtcg gcatccgcga
     3121 tgaccttgcg atagttctcg aaggtccagc cggtgccgaa cgggttgaac aacgcattcc
     3181 accagccgtt ggactgtgcg tcgtcgcggc tgcggaagga ggtgacgagc agacccagtg
     3241 tgggcagggt ccagatgatg cacagcagga tcatcgagat gatcgagaac atgctgcgcg
     3301 gccggccgtc cttcatcagc ctgcgctgac gcggcttggg gtccgagacg acgggtgcgg
     3361 gggtcatagc cgtggaactc atcggttcat ctgctcctca cggaaggtct tgacctggta
     3421 ccagaggatc ggcagcacgg cgatgagcag cacgacgacg atcgccgacg cgcggccggc
     3481 ctggctgttg gcgaacatct cgttgaagaa gaggttcgcg atgacatcgg agttgtcgcg
     3541 gccgttggtc aggacgtaga cgatgtcgaa gaccttcatc acgaggatga ccacggtgat
     3601 gaagacggtg acgatggtgc cgcggatctg cgggatgatc accttgaaga agatctgcag
     3661 ctcgctggcg ccatcgatcc gggccgcctc gatggtgtcg tccgggacgc ccttgatggc
     3721 agaggacagc aggatcatcg agaagccgac ctgcaaccag atgaggatga ccatcatcaa
     3781 cagcgagttg agcttgccgt cggatatctg cagccacgtc ttggggtcca tccccagctt
     3841 ggtgacgatc gcgttgagca gaccgacctg gctttgcgac ttgtcgctgt aggcgtagac
     3901 gaggttccag atcgccgaag caccgacgaa gctgatcgcc atggggagga agatgatcga
     3961 cttgctgacc ttctcaccct gcgccgagag cttgtcggcg agcacggcga cgatgatgcc
     4021 gatcgcgacg gtgatcgctg ggacgatcgc caaccacagg aggttgttca gggtggcgtt
     4081 ccagaagtcc ttcgacccaa agatgtcctt gtagttcgcg agcccgacgt attcggtcga
     4141 gttggcgttg gcgaaggagt agttgatcgt ctggatcgtc gggtagatga gcatgagggc
     4201 gatgagcccg aagcccggca gcaggaaggc gtagggcatg atgccctggc tgagccggtt
     4261 gggcaggccc tcgatgaaca tattgaggaa ccagaagagc aacgcggccc caccgacccc
     4321 gccgatcagg gcgagtgcgg cgatgaggat cttgttgtcg aggaaccact gggggtagta
     4381 gccgaacgcg aggaagagat tgcccagcgc ccaggcggtg aggatcaggc cgaagagccc
     4441 gatgacgatc ttggggacat tggcccggtt gatgtcatcg gggtcccgtc gctgcggggc
     4501 gacgacggta tcggttgcgg tgctcatcag gacttcggcc agctgttctc gatcgcggtg
     4561 gcggtctcct cgtcagaggc gccattcatc cacttgacca tctcggtcca gaacgtgccc
     4621 gagccgacct ccttgggcat caggtcggag gcatcgaagc ggaagacatc cgcgctcgac
     4681 acgatctcgg ccatctgctt cgtcagctca ttggggtagt tcgtcgcgtc gaaggtcttg
     4741 tgcggcgaca gccagccgcc gaccttggcc cagggcccgc cgaacttgtc ggaggtgagg
     4801 aacttcatga cctcgatggt gtcggggtcg ttgccgttga actgcgccgc caagtcgcca
     4861 ccaccgagga tgggctggcc gccgtaaccg ccgtcgagcg gcgggaagac gaagatcccg
     4921 accttgttgt cgaggtctgc ctggacgtcc ttggggagga agccggtcgc gaagttgccc
     4981 tgtcgcatca agtagcactt cggcgggtcc tggaacgccg gggtcatcgc gtcaccgaac
     5041 ttggtgttga tgatgttcgc ggcgccaccg aagacattgc cgtcaccctt ggcgagcttg
     5101 ccgaactcgg caaacgcctt gaggatctgc gggtcgttga acgggatctt gtggctgacc
     5161 cactggtcgt agacgtccgg gccgctgact cgcagcacca tctcctcgat ccagtcggtg
     5221 ccgacccagc cggtggctgc gtcgctaccc cagcccatgc accagggcgc ggtgcctttg
     5281 cccttgatct cgtcggtgtt cttcgcgagg tcttgcaggg aggtgaactc ggtcttgccg
     5341 ccggcttcgg tccacgcagg cttgggatac cagacgatgg acttgacggc catgcgcatc
     5401 ggggcgccat aggagcggcc cttgtaacgg gtggactcga ggaagccggg caccaaggtg
     5461 gtggccaggg tgtcgtagtc gaggacggag tcgatgggct ggatcttgcc ggcctcggcc
     5521 agttcgatca gaccaccggg ctgcgggaag agcgcgatgt cgggcgcatc gccggcagtc
     5581 accctggtct tgacgatggt cgtgaagttg ttgtcgtccg tgtactggat gtcgatgccg
     5641 gaggacttct cgaattcggc gagggaggcc tcgaaggcct tcttctcatc gccaccgaag
     5701 gcgccgagga tggtgacgac cttgtcgccg tccttctgcg tgttgtcgac gtagccgttg
     5761 agtccggccg cgccaccgcc gtcggaggcg ttcgggttct ggaggcaggc gctcaacaag
     5821 gtggtggtga ggatcgcacc ccccatcgcg acgagttgcc tgcgccggtg cacctgcgcc
     5881 atgtgatctc cttcgattca cgcgttggcg agggcatacg aagtagccag cgccacgctg
     5941 gttggtcccc ctgttgtaac cccaaccctt gtcaaccgtc caggcaccac gacgggtcga
     6001 taccgcttcg tgacatagtt ggcacgttca aattctcgct tcgcgagatt gacggtatga
     6061 cgtgtggccg gtgtgatccc accagccacc tgggccccgt gggcaccagg taggtgagac
     6121 gctgcgtgcc gcggcgcggc tcagcgcgat tggatatggt ggatccaggc ctcgacctcg
     6181 gcgctgcttc gcggcatacc ggcggagagg ttctcgcatc cgtcttcggt cacgaggacg
     6241 tcgtcctcga tccgcacacc gatgccgcgg aagcgctccg gcgccaggtc gtcgtccgcc
     6301 ttgaagtaca gccccggctc gacggtgagg atcatcccgg ggcgcagttc ggcgtccatg
     6361 tactcctcgc gcgtggccag cgagcagtcg tgcacgtcga ggccgaggtg gtggctcgtg
     6421 ccgtggacca tccagcgccg gtggtactgc ccgtggtcct tgtcgagcgt ctcctcgacg
     6481 ccgatgccct ctggcagcag cccccacgcg tgcaagtgct cggcgatgac ccggatcgcc
     6541 gcagcgtgca cgtcgctgaa cttgtttccc ggcttgaccg cggcgatgcc ggccgcctgc
     6601 gcggcgcaca ccgcgtcata cacctcgcgc tggtcctcgg tgaaggtgcc gctgacgggc
     6661 agggtgcgcg tgatgtccgc ggtgtagagg gcgtcgacct cgacgcccgc gtcgagcagg
     6721 atgagatcct cctcggtcac gtcgccggtg ttcttgatcc agtgcagggt gttcgcgtgg
     6781 tcgccggccg cgcagatcga gtcgtagccg acgccattgc cctggtggcg tgcgtgcagc
     6841 ccgaagacgc cctcgaccca ccgctcaccg cggcccttgg ccaccaggtg cggcaggtcg
     6901 gtgatcactg cctcgaaccc caccttggta gcggcgatgg ccgcgcgcat ctgctcgatc
     6961 tcgaacgcgt ccttgaccat ccgtgacgtc gaaatcgtat gcgccagttc gtcatcggcc
     7021 tcttgcagct gttcttgctc gcgcccttcg gacacccggc gctcgtcgag ctgcgccgtc
     7081 agtgcgctgt cggcgtcgcg caccacacgc acgatcattt ccgcggcgtc cttggtcacg
     7141 gcatcgagga agtcgtcgat gtgccgcccg ctcaggtcga gttcggcctc gacgtcctcg
     7201 atgctcggac gcggcccgac ccagaactcc ccatagcggg cgtcgccgaa gaactcgtcg
     7261 ctgtcgcgcg ctgccagagg ccggaagaag aggatcgcct cgtggccatc ggcggtcggt
     7321 tcgagcacga gcaccgcgtc cggttcgcgg tcggcgccga gcccggtcag ataggcgaag
     7381 gcggtatgcg gtcggaagac gtagtcggtg tcgttgctgc gcaccttcag cccgccggcg
     7441 gggatcacca ggcgttcgcc gggataggcg gcggacaggg tggcacggcg ggcggcggca
     7501 tacggcgcgg cgggggagcg gcgggggcgc gcgtcggcgc gcggcgccca acccgtctgg
     7561 acgaaggcgc ggaactcctc ggtcgtctgc cgggcccgat tgtccgccgt cttctgctcc
     7621 tcgctcatgg cgccatggtg tcacgcaaac cgcgccccta cggggacacc cttag
//
LOCUS       DAOPJG010000168         7652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_10217_length_7652_cov_7.139265, whole genome shotgun sequence.
ACCESSION   DAOPJG010000168 DAOPJG010000000
VERSION     DAOPJG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10217_length_7652_cov_7.139265"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKU75_13290"
     CDS             <1..289
                     /locus_tag="PKU75_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HPF81899.1"
                     /translation="HSNVDSDEVMFYVDGDYEARKGSGIGIGSVSLHPGGHAHGPQPG
                     AMERSLGAEYFDETAVMVDTFKPLELGEGALACDDGRYLLSWHTTNSVPEP"
     gene            322..612
                     /gene="clpS"
                     /locus_tag="PKU75_13295"
     CDS             322..612
                     /gene="clpS"
                     /locus_tag="PKU75_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HPF81900.1"
                     /translation="MSIAPVAEEVTSAGTDEALDVPWITLVWNDPVNLMSYVTYVFQS
                     YFGYTREKSEKLMLDVHTKGKAVVSTGPREKMEIDTEALQGYGLWATFQKDI"
     gene            615..1274
                     /locus_tag="PKU75_13300"
     CDS             615..1274
                     /locus_tag="PKU75_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HPF81901.1"
                     /translation="MARGFVKRGKGAGVRYAARLDEAERALLIELFGQVRDLIVPPGS
                     VAAQPDTPPEGDDPFDAIVAGLGADFGNVTVPDSPADLGGRGFGAEDRDPALDRLFPA
                     ANRTDEEAAKEFRRLTEASLRDRKISGLDAACAALAKSVDDRFELSEDKARSFLVALT
                     DVRLVLGDRLGLREDADAQRLQEIAEIADPADPAAYAIVVYDFLTWLQETLATSMLPK
                     R"
     gene            1296..2117
                     /gene="murI"
                     /locus_tag="PKU75_13305"
     CDS             1296..2117
                     /gene="murI"
                     /locus_tag="PKU75_13305"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPF81902.1"
                     /translation="MDAPIGIFDSGYGGLTVARAVLDQLPHESVAYLGDTARAPYGPR
                     PIAETRQFALECLDRLVDHGVKILVIACNTASAAVLHDARERYDVPTIEVIRPAVRRA
                     ARATRNQRVGVISTKGTHTSRAYIDAFAAAPDIDVFSKPCPRFVEFVESGITGGPELL
                     TVAHEYLDPLAEKDIDTLVLGCTHYPLLTGVISYVMGEGVTLVSSAEETAKDVYRTLA
                     DLDALRPDELPPPTHGFSTTGDPEEFRRLARRFLGPEVGDVFGNLAQAPLAVGVP"
     gene            2114..2878
                     /locus_tag="PKU75_13310"
     CDS             2114..2878
                     /locus_tag="PKU75_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPF81903.1"
                     /translation="MKLTVIGCAGSFAGPASPASCYLVQAEQDGRLWSILLDMGNGSL
                     GALQRHLDPCAVDAVFLTHLHPDHCLDLTGFYVYRKYHPGGPCTHRVPVYAPAGAEDR
                     IHAAYEGIDADGMNAQLDFRVVGEGSVVEVGPFRLTTHAMNHPVETYGYRVEAGGSVL
                     AFTGDTDTCPGLLTLAAHADLLLTDCAFVDGRDPLPDIHLSGSRAAAAAVAAGAKRLM
                     LTHIPAWNDPQVCRAQAAAVWPGEVELAAPDANYVI"
     gene            2902..3648
                     /gene="rph"
                     /locus_tag="PKU75_13315"
     CDS             2902..3648
                     /gene="rph"
                     /locus_tag="PKU75_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPF81904.1"
                     /translation="MTRHDGRAADALREIRITRNWLDHAEGSVLVEFGRTRVLCAASF
                     TEGVPRWLKGKGTGWVTAEYEMLPRSTNTRSDRESRKGKVGGRTHEISRLIGRSLRAI
                     IDTKALGENTIVLDCDVLQADGGTRTAAITGAYVALADAIADAHARGLIPASAHPLTG
                     SVAAVSVGVVEGVPVLDLDYPEDSTAETDMNVVMTGDGRFVEVQGTAEGEPFDRDLLG
                     ALLDLATTGIEELTRLQAAALDAPARERVR"
     gene            3645..4286
                     /gene="rdgB"
                     /locus_tag="PKU75_13320"
     CDS             3645..4286
                     /gene="rdgB"
                     /locus_tag="PKU75_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPF81905.1"
                     /translation="MSRRIVLATRNEHKVHELRQILSAVCRDLGLEIVGAADFPEVPD
                     VPETEVTFVGNARLKAEALAAATGLPALADDSGLAVEVLGGCPGVFSARWSGSHAGAD
                     APRAEKDRANLTLLLEQVADVPEEHRGAAFVCAAVLAIPGQDSATCEGRVTGRLAYGP
                     RGTNGFGYDPVFIPDADPARTLAEFSDTEKNAISHRGNAFRAMEPVLRERLGG"
     gene            complement(4290..4832)
                     /locus_tag="PKU75_13325"
     CDS             complement(4290..4832)
                     /locus_tag="PKU75_13325"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPF81906.1"
                     /translation="MTIPTGSYDLSADNGALLVKTFTDGPAAAMGHNLVIEATSWAAQ
                     VEIGKTPAASRLSMTADLTSLEVRDGTGGAKPLSAGDKSKILDNAAKALNAKKHPALS
                     FTATDISGTWDSGVVRGDMTINDTTAPLDLTLDQPAPGELRLTGTIVQSDFGIKPYST
                     MMGALKLKDAVDVEVSIPFE"
     gene            complement(4902..4983)
                     /locus_tag="PKU75_13330"
     tRNA            complement(4902..4983)
                     /locus_tag="PKU75_13330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4947..4949),aa:Leu,seq:tag)
     gene            5027..5497
                     /gene="bcp"
                     /locus_tag="PKU75_13335"
     CDS             5027..5497
                     /gene="bcp"
                     /locus_tag="PKU75_13335"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPF81907.1"
                     /translation="MSIRLEPGDTAPDFTLTDDTGAPVSLADFRGRKVILYAYPKAMT
                     PGCTTQACDFTESLDRLQGEGYDVVGVSPDSPASLAKFRERDQLTIRLLSDPDKAMLT
                     AYGAFGEKKNYGKVVQGVIRSTFVVDEAGKIANAWYNVKATGHVAKLRRDLGLD"
     gene            5522..5782
                     /locus_tag="PKU75_13340"
     CDS             5522..5782
                     /locus_tag="PKU75_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HPF81908.1"
                     /translation="MSDPSVEKLRADIAASRERLAQTVDELTTKAAPKAIVQRQTESA
                     KARFAAATTTPSGDLRTERIAAVAAALAVVLVVRCILRRRSS"
     gene            5834..6133
                     /locus_tag="PKU75_13345"
     CDS             5834..6133
                     /locus_tag="PKU75_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPF81909.1"
                     /translation="MLHDRLLVSLEGQEGERRSGGGILIPATASVGRRLAWGEVVATG
                     PNARQVEVGDRVLFDPEERGEVELQAKSYTLLRERDLHAIAARRVSGEGHTGLYL"
     gene            complement(6288..6845)
                     /locus_tag="PKU75_13350"
     CDS             complement(6288..6845)
                     /locus_tag="PKU75_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPF81910.1"
                     /translation="MPLPALPGLTSLPLPERLDLVAARAAAALSADPEFAGRLAVVEI
                     DPALADTEALVAAYDLDLAASGNCVVISGSRGGEERIAACVVPADTRADVNNLAKRRL
                     DVRKASFHPMERAVAETGMEYGGITPVGLPAGWRIFVDAALTGQDVVVIGSGLRRSKL
                     LIPGAAFADLPGAEIVDGLGRPVTT"
     gene            6896..7543
                     /locus_tag="PKU75_13355"
     CDS             6896..7543
                     /locus_tag="PKU75_13355"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HPF81911.1"
                     /translation="MPAPRRRIRSALIALLLAVFSVCLAGPAGAHSDLESSSPKAGAT
                     LTELPTEVTLTFNEKVQEGFSQVAVVDGEGLSVTDGDPVSDGATVTQPLMAKLHPGGY
                     VINFKVVSADGHPISDAIPFTIAEGAGSASATESDSPTSAEASASAPAATTPAATGET
                     AATTAATGTSSTGPAGGGDGSGGGPATALLVILGVLVLAGGMGAAAMLRARGPQA"
BASE COUNT         1156 a         2760 c         2599 g         1137 t
ORIGIN      
        1 ccactccaat gtcgactccg acgaggtcat gttctacgtg gacggcgact acgaggcccg
       61 caagggcagt gggatcggca tcggctcggt gtcgctgcac cccggcggcc acgcccacgg
      121 cccgcagccc ggggccatgg agcgctccct cggcgcggag tatttcgacg agaccgcggt
      181 catggtcgac accttcaagc ccctcgaact cggcgagggc gccctcgcct gcgacgacgg
      241 acgctacctg ctctcctggc acacgacgaa ctccgtcccg gagccgtaag cgctcgcgtc
      301 acgagcccct aagctgggcc catgtcgatc gcgcccgtgg cggaggaggt cacctccgcc
      361 ggcaccgacg aggcgctgga cgtcccgtgg atcaccttgg tctggaacga cccggtcaac
      421 ctgatgtcct atgtcaccta cgtcttccag agctacttcg gctacacccg cgagaagtcc
      481 gagaagctca tgctcgatgt ccacaccaaa ggcaaggcgg tcgtgagcac cgggccgcgg
      541 gagaagatgg agatcgacac cgaggccctg cagggctacg gcctgtgggc gaccttccag
      601 aaggacatct gagcgtggcg cgcggattcg tcaaacgtgg caagggcgcc ggggtgcggt
      661 atgccgcccg cctggacgag gcggagcggg ctctgctcat cgagttgttc ggtcaggtcc
      721 gcgatctgat cgtgccgccg ggttccgttg ccgcgcaacc ggatacaccg ccggaggggg
      781 acgatccctt cgacgcgatc gtcgccgggc tcggcgccga cttcggcaat gtcaccgtgc
      841 ccgattcacc ggccgatctc ggcggccgag ggttcggtgc cgaggaccgg gatccggcgc
      901 tcgatcggct cttccccgcc gcgaaccgca ccgacgagga agcagccaag gagttccggc
      961 gcctgaccga ggcgtccctg cgcgaccgca agatcagcgg tctggacgcg gcgtgcgcgg
     1021 ccctggccaa gagcgtcgac gatcgcttcg agttgagtga ggacaaggcg cgttcgtttc
     1081 tcgtcgccct caccgacgtg cgcctcgtcc tcggcgaccg gctcggcctg cgcgaggacg
     1141 ccgacgccca gcgcctccag gagatcgcgg agatcgccga tccggccgac cccgcggcct
     1201 acgccatcgt cgtctacgac ttcctcacct ggctgcagga gacactcgcc acgtcgatgc
     1261 ttccgaagcg ctgacgctgg cttagggtct ccttcgtgga cgcacccatc gggatcttcg
     1321 attccggcta cggcggcttg acggttgccc gagcggttct cgatcagctg ccccacgagt
     1381 cggtggccta tctcggcgac accgcccggg cgccatacgg cccgcggccg atcgccgaga
     1441 cgcgtcagtt cgccctcgaa tgcctcgacc ggctcgtcga tcacggcgtg aagatcctcg
     1501 tcatcgcctg caataccgcc agcgcggccg tgctccacga cgcccgcgag cggtatgacg
     1561 tgccgaccat cgaagtgatc cggccggcgg tgcggcgggc cgcccgcgcc acccgcaacc
     1621 agcgtgtcgg ggtgatctcg acaaagggca cgcacacgtc gagggcctat atcgacgcct
     1681 tcgctgcggc ccccgacatc gacgtcttct ccaagccgtg cccgcgcttc gtcgagttcg
     1741 tcgagtccgg gatcaccggc gggccggagc tgctcacggt ggcgcacgag tacctggatc
     1801 cgttggcgga gaaggacatc gacaccctcg tcctcgggtg cacgcactac cccctgctca
     1861 ccggcgtgat ctcctatgtg atgggggagg gcgtcaccct cgtcagctcg gcggaggaaa
     1921 cggccaagga cgtctaccgc accctggccg acctcgacgc cctccgcccg gacgagttgc
     1981 cgccgcccac gcacgggttc tccaccaccg gagaccccga ggagttccgc cgcctcgccc
     2041 gccggttcct cggcccggag gtcggggacg tcttcggcaa tctcgcccag gccccgctcg
     2101 cggtcggtgt cccatgaagc tgaccgtcat cggctgcgcc gggtccttcg ccggcccggc
     2161 gtccccggcc tcgtgctatc tcgtccaggc cgagcaggac ggccggctct ggtcgatcct
     2221 cctcgacatg ggcaatgggt ccttgggtgc gttgcagcgc cacctcgacc cgtgcgccgt
     2281 ggacgccgtc ttcctcaccc acctgcaccc ggatcactgt ctggacctga ccggtttcta
     2341 cgtctaccgc aagtaccacc cgggggggcc gtgcacacac cgcgtccccg tctacgcccc
     2401 ggcgggtgcc gaggaccgca tccacgcggc atacgaaggc atcgacgccg acgggatgaa
     2461 cgcgcaactc gacttccgcg tcgtcggcga gggcagcgtc gtggaggtcg ggccgttccg
     2521 gctcacgacg cacgcgatga accatcccgt cgagacctac ggctaccgcg tcgaggcggg
     2581 cggctccgtg ctcgccttca ccggcgacac cgacacctgc ccgggcctgc tcacgctcgc
     2641 cgcgcacgcg gacctcctgc tcaccgactg cgcgttcgtc gacggccgcg acccgctccc
     2701 ggacatccac ctcagcggga gccgggcggc ggcggccgcc gtcgccgccg gcgcgaaacg
     2761 gttgatgctc acccacatcc ccgcgtggaa cgacccgcag gtatgccgcg cgcaagccgc
     2821 cgctgtctgg cccggcgagg tcgaactcgc cgcgccggat gcgaactacg tgatctgaga
     2881 acgtccggtt acggtgtcgc catgacccga cacgacggcc gcgccgccga cgctctccgc
     2941 gagatccgca tcacccgcaa ttggctcgac cacgccgagg ggagcgtgct ggtcgagttc
     3001 gggcgcactc gcgtgctgtg cgccgcatcc ttcaccgagg gtgtgccccg ctggctcaaa
     3061 ggcaagggca cgggctgggt cacggcggaa tacgagatgc tgccccggtc gaccaacacc
     3121 cgctccgacc gggagtcccg caagggcaag gtcggcgggc gcacccacga gatctcccgg
     3181 ctcatcggcc ggtccctgcg ggccatcatc gacacgaaag cgttggggga gaacacgatc
     3241 gtgctcgact gtgacgtcct gcaggccgac ggcggcaccc ggacggccgc gatcaccggt
     3301 gcctatgtcg ccctggcgga cgccatcgcc gatgcccacg cccgcggtct catcccggcc
     3361 tcggcgcacc cgctcaccgg gtcggtcgcg gcggtcagcg tgggcgtggt cgagggcgtg
     3421 cccgtgctcg atctcgacta cccggaggac tcgaccgccg agacggacat gaatgtcgtc
     3481 atgactggcg acgggcggtt cgtcgaggtc cagggcacgg ccgaggggga gcccttcgac
     3541 cgggatctgc tcggggcgct gctcgacctc gccaccacag ggatcgagga attgacgcgc
     3601 ctgcaggcag ccgccctcga cgcgccggcc cgggagcggg tgcggtgagc cggcgcatcg
     3661 tgctggcgac gcgcaacgag cacaaggtcc acgagctgcg ccagatcctg tccgccgtct
     3721 gccgcgacct gggcctggag atcgtcggcg ccgccgactt cccagaggtc ccggacgtgc
     3781 cggagaccga ggtcacgttc gtcggcaacg cgcgcctgaa agccgaagct ctcgccgccg
     3841 caacgggttt gcccgccctc gccgacgact ccggccttgc cgtcgaggtg ctgggcgggt
     3901 gtcccggcgt cttcagcgcc cgctggtcgg gcagccacgc cggcgccgac gccccgcgcg
     3961 ccgagaagga ccgggccaat ctcaccctgc tgctggagca ggtcgccgac gtccccgagg
     4021 agcaccgcgg cgccgccttc gtctgcgcgg cggtgctggc gatccccgga caggactcgg
     4081 cgacctgcga gggccgggtg accgggcgac ttgcatacgg cccgcggggc accaacggct
     4141 tcggctacga cccggtcttc atccccgacg ccgacccggc ccgcacactc gcggagttca
     4201 gcgacacgga gaagaacgcg atctcccacc gcggcaacgc cttccgcgcg atggagccgg
     4261 tgctgcgcga acgactcggc ggttgagagt cactcgaacg ggatcgagac ctcgacgtcg
     4321 acggcgtcct tgagcttcag cgcacccatc atcgtcgagt agggcttgat gccgaagtcg
     4381 ctctgcacga tggtgcccgt caggcgcagt tcaccgggtg cgggctggtc gagggtcagg
     4441 tcgagggggg cggtcgtgtc gttgatggtc atgtcgccgc ggaccacgcc ggaatcccac
     4501 gtcccggaga tgtccgtggc ggtgaagctc agcgcggggt gcttcttggc gtttagggct
     4561 ttggcggcgt tgtcgagaat cttgctcttg tcgcccgcgc tgaggggctt ggcgcctccg
     4621 gtgccgtcgc ggacctccag gctggtgagg tcagccgtca tcgacagcct cgacgccgcc
     4681 ggtgtcttgc cgatctcgac ctgggccgcc caggacgtgg cctcgatgac gaggttgtgg
     4741 cccatcgcgg ctgccgggcc gtcggtgaac gtcttgacga gcagggcgcc gttgtcggcg
     4801 gagaggtcgt agctgccggt gggaatcgtc atggtgccag tctgctcctg cagggccggc
     4861 cggggagatc gggggcgtgt gatgtcccga gactcactca gtgcgcgagg cgggacttga
     4921 acccgcacac ccgaaggcac cagaacctaa atctggcgcg tctgccaatt ccgccactcg
     4981 cgcgcgaacg acaactctag atcagcctcg gggtaggttg acgcgtgtga gcattcgtct
     5041 cgaacctggc gacaccgccc cggacttcac gttgaccgac gacaccggtg cgccggtctc
     5101 gctcgccgac ttccgcggcc gcaaggtcat cctctacgcc tacccgaagg cgatgacgcc
     5161 cggctgcacc acccaggcgt gcgacttcac cgaatcgctg gaccggttgc agggcgaggg
     5221 gtatgacgtg gtcggcgtct ccccggacag cccggcgtcg ctggcgaagt tccgcgagcg
     5281 cgaccagctg accatccgcc tgctgtccga tccggacaag gcgatgctca ccgcatacgg
     5341 cgcgttcggc gagaagaaga actacggcaa ggtggtccag ggcgtgattc gctcgacatt
     5401 cgtggtcgac gaggccggca agatcgccaa cgcgtggtac aacgtcaagg ccaccgggca
     5461 cgtcgccaaa ctgcgtcgcg atctcggcct cgactgaccg gcagattgaa ggagaacccc
     5521 catgagtgac ccgtcggtcg agaagctgcg cgccgatatc gccgccagcc gggagcgcct
     5581 cgcgcagacc gtcgacgaac tgaccaccaa ggccgcaccc aaggcgatcg tgcagcgcca
     5641 gaccgagtcg gccaaggctc ggttcgcggc ggcgacgacc acgccgtccg gggacctgcg
     5701 caccgagcgc atcgccgcgg tcgcagcggc cctggccgtc gtcctcgtcg tgcgctgcat
     5761 cctgcgccgc cgctcctcct agacgagcgt ggccaaggcc aagggcgagg cagcccccgg
     5821 gctgcccatc cgtatgctgc acgatcgcct cctcgtctcc ctcgagggtc aggagggcga
     5881 gcggcgctcc ggcggcggca tactcatccc cgcgacggcc agcgtcggcc ggcgcctcgc
     5941 ctggggcgag gtcgtcgcca ccggccccaa cgcccgccag gtcgaggtcg gcgaccgggt
     6001 gctcttcgac cccgaggagc gcggcgaggt cgaactgcag gccaagtcct acaccctcct
     6061 gcgcgaacgc gacctgcacg ccatcgccgc ccgccgcgtg tccggcgagg gccacaccgg
     6121 tctctatctc tgagccccca ccggacgaac tggcccccgc tcgggacagc cagaatacga
     6181 aggacaggtt ttgaccggtc gaagcgccgg tattcggctc tcctcgtatt ccgaatgtca
     6241 ccattccgaa tgtcaccagg tcggtccggc cgacgccgtg cccggcgtta cgtggtgacg
     6301 gggcgcccca ggccgtcgac gatctcggcg cccggcagat cggcgaaggc cgcacccggg
     6361 atgagcagct tgctgcggcg caggccgctg ccgatcacca cgacgtcctg cccggtcagg
     6421 gcggcgtcga cgaagatgcg ccagccggcc ggcagaccga cgggggtgat gccgccatac
     6481 tccatgcccg tttcggcgac ggcgcgctcc atcgggtgga aggacgcctt gcggacgtcg
     6541 aggcggcgtt tggccaggtt gttgacgtcg gcgcgggtgt cggcggggac gacgcaggcg
     6601 gcgatgcgtt cctcgccgcc gcgggagcca gagatgacga cgcaattgcc gctggccgcg
     6661 agatccaggt cgtatgccgc gacgagcgcc tcggtgtccg cgagcgccgg gtcgatctcg
     6721 acgacggcga ggcggcccgc gaactcgggg tcggcgctca gcgccgccgc agcccgtgcg
     6781 gcgacgagat cgagccgctc ggggaggggc agtgaggtca ggccggggag ggcgggcaac
     6841 ggcataccgg aaccctaatc ggacctgggg gtccggttca gggacaatgg cacgcgtgcc
     6901 cgcccctcgc cgacgcatcc ggtccgccct gatcgccctc cttctcgccg tcttctcggt
     6961 gtgtctcgcc gggcccgccg gcgcccactc cgatctggag tccagcagcc ccaaagccgg
     7021 ggcgacgctg accgagttgc ccaccgaggt caccctgacc ttcaacgaga aggtgcaaga
     7081 gggcttcagt caggtcgccg tcgtcgacgg ggagggactg tcggtcaccg acggcgaccc
     7141 ggtcagcgat ggtgcgaccg tgacccagcc gctcatggcg aagctgcacc ccggcggcta
     7201 tgtcatcaac ttcaaggtgg tcagcgccga cgggcacccc atcagcgacg ccatcccgtt
     7261 cacgatcgcc gagggcgccg gctccgcttc ggcgacggag agcgattccc ccacgagcgc
     7321 cgaggcgtcg gcctccgcgc cggccgcgac caccccggcc gccacgggcg agaccgcagc
     7381 caccactgcc gcgaccggca cgtcctcgac cggcccggcc ggcggcggtg acggatcggg
     7441 cggaggaccc gcgacggccc tgctcgtcat cctcggagtg ctcgtcctcg ccggcggaat
     7501 gggtgcggcc gccatgctcc gcgcccgcgg tccacaagcc tgagccgggc ccggggaaac
     7561 cgatttcgtt gggcccggga cggggtgcta gtgtcttccc tcgcgcgcca ttagctcaat
     7621 tggcagagca gctgactctt aatcagcggg tt
//
LOCUS       DAOPJG010000169         7542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_10427_length_7542_cov_7.599573, whole genome shotgun sequence.
ACCESSION   DAOPJG010000169 DAOPJG010000000
VERSION     DAOPJG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10427_length_7542_cov_7.599573"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKU75_13360"
     CDS             complement(<1..392)
                     /locus_tag="PKU75_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81912.1"
                     /translation="MTGRAPGAGLPSILTLNLSRPPVGRAKALLEYLWLRAEEVLVLT
                     ETGWGAGSDLVASVCRGAGYSVQSSLDDGSGRRRRLASGDRAPYGVLVVSRGPELTRA
                     DGLDVPLMPERVLVTEMPGPAGPVRLIAV"
     gene            complement(389..625)
                     /locus_tag="PKU75_13365"
     CDS             complement(389..625)
                     /locus_tag="PKU75_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81913.1"
                     /translation="MSERQPSTPNAVTGGALIGIGGLILLRVVGPARLLSLLFLAAGA
                     FYLNRTDPKRLTAVLIVVGVGLGWVAYRTASQRL"
     gene            693..1196
                     /locus_tag="PKU75_13370"
     CDS             693..1196
                     /locus_tag="PKU75_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPF81914.1"
                     /translation="MTDSTAGEHPVVEQRGLWFEEFEVGTVYRHRPGRTVTEADNVLF
                     TTLTMNTQPLHLDEADSAARPPFHARLVNSMFTLSTLVGLSVSQLTLGTIVANLGFSE
                     IAFPAPVFHGDTLYGETLVVDKRPSKSRPGEGMVTLRHTARNQDGVVVATATRATLVR
                     MRPEEER"
     gene            1193..2017
                     /locus_tag="PKU75_13375"
     CDS             1193..2017
                     /locus_tag="PKU75_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPF81915.1"
                     /translation="MSESTAVWTPGPAWLFCPADRPDRYAKAAERSDVAIIDLEDAVA
                     PQDKPAAREALAAAYGGLDPERTLVRINPAATADHALDVDLVQRAGIRRVMLAKTESA
                     ADLAALGACEVVVLLETPRGVVAAREILEAEPVVGAMWGAEDLVAGLGGTSSRDAAGA
                     YRPVAVQAQCTALLAAKAAGKLAVDAVHLAITDHDGLRVESEAAVASGFDAKALIHPT
                     HVDIVRAAFRPDPAQVAWAERVLEGEKQHGSGVFALDGAMVDGPVLAQARRVLARS"
     gene            complement(2014..2622)
                     /locus_tag="PKU75_13380"
     CDS             complement(2014..2622)
                     /locus_tag="PKU75_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPF81916.1"
                     /translation="MIAMHPEAVAGDPASVRWVVAQAPAGNGPVTAVPEPLAGLVADG
                     TITALQLLPGAVLVTLGPGRSWRAEGARVRSALHAALEQPQAWRAGAAEVSVEEITAV
                     LAGPAGDVVRAHGGAVWVAGVHAGVVDLELSGACDGCPASGLTISTTLAAAIRRAVPG
                     VVDVRLIESRRPAFVSLGALLRRGPQEPDAAGPACRRGVAAL"
     gene            complement(2634..4580)
                     /locus_tag="PKU75_13385"
     CDS             complement(2634..4580)
                     /locus_tag="PKU75_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPF81917.1"
                     /translation="MSAHQHHGQGGGASALSGLRLALIGSPNCGKTTLFNALTGLHGK
                     TGNYPGVTVARYEGPARAHGIPLIVEDLPGTYSLDPISLDEQVVIDALDPEVAASPTP
                     DGLLVLLDVTTLRRSLPLLAQVQVSEQPLCVILTFGDELAARGGHVDVGKLQRALGLR
                     VIPVTAGDPAGLAALKDAAAEIDSWPRPPLPPPVEGPEGAAWIDSVLAAADYRPAGAD
                     RRTQKLDAVLLHPLLGGLIFFAVMFAFFQIIFTVAAPLQGWIEQFFGWLGALAAARLG
                     HGWAGEFVGTALLGGIGGVLVFLPQIALLFLMISLLEGVGYLSRAAFIMDRFMARFGL
                     EGRAFVSLLSSVACAIPGIMATRTLPSARDRLATMMSAPLMTCSARLPVFVLLVSMLI
                     PASEQVGPFGARGVVLFALYVVGALSALLTAALFTRVGRRTGQALPFYMELPPYRIPS
                     LRVVLFSVWDACRSFLRKVTHIILATTLLLFLLLNLPARSADDMRAAGVDPADPAASA
                     AYVLDNSFAADIGRAVGPVFAPLGFDWRINVGVVSSLAAREVFVATMGQVAAAEDPEN
                     PGQALRAMRVADGPRAGERLFTPPTIAALLAFFVYALQCVSTIGVLRRESGSWRWPLL
                     AFGYMFTLAWVAAFLARTITAAVT"
     gene            complement(4577..4864)
                     /locus_tag="PKU75_13390"
     CDS             complement(4577..4864)
                     /locus_tag="PKU75_13390"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPF81918.1"
                     /translation="MASRLGKRRVPVAARVPRTLDSVAIGEAGVTVSGFRADVAPEIG
                     RRLGDLGFVVGAPVTVVRRAPFSGPVIVDIAGYEIALRVGEARRIVVSEAR"
     gene            complement(4977..>5729)
                     /locus_tag="PKU75_13395"
     CDS             complement(4977..>5729)
                     /locus_tag="PKU75_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81919.1"
                     /translation="GPDERPTLAGASALTPSSWEGDPQPEGQGAAYPQRPLPPRPAMA
                     GDPRIGRASRGGVLLALGAALVAGAAGHPALALVVGLGWSVLARTADRSVTSLVLRRH
                     HKGERPSDLAMSVLSSPVHLLIGLVATLAGALIPLAVGLAGVFATVLAARMADVAGPE
                     RLTYAVALAVGMALAVMMSWWGPGGAGLRRGSRSLARGLAPGDVFAQGVTGVLLAAAT
                     GLAVWAGMSADLPLWWPLTGAPDILQSLTTAP"
     assembly_gap    5730..5829
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5830..7022)
                     /locus_tag="PKU75_13400"
     CDS             complement(<5830..7022)
                     /locus_tag="PKU75_13400"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPF81920.1"
                     /translation="MSEQPAGELPEFTRIGPYRLLQQLGEGGMGVVHLALDRAGRAVA
                     IKVLRPHVAADPAARTRLAREVDTLSRIRHPNVAPIIDHDVEGERPYIVTRYIAGPGL
                     DEVVDDAGPLRGAALLQVGRGLAGALHAIHEAGVIHRDVKPGNILLLDGEPILIDFGI
                     AHVADDVRLTRTGLVMGTPGYLSPEIVEGAAVTDSTDWWGWAATLGFAAQGRPPFGRG
                     SMETILSRVVRGEADLAGVDPDLVPLLEAALSPYPEDRPTDTEVLRALERYAAGGQVT
                     DVLRVVTRREAPGTSAAATPVTELLSGPATAVLPPVAPPPPRVVAPAPAPAREPAAQP
                     SYAGGTAYDRGTAYAGGTAYDGSPQPRPAYTRAPAYDPRDDPRAHAAAPAAPPGRSDT
                     PGRPAP"
     gene            complement(7078..7506)
                     /locus_tag="PKU75_13405"
     CDS             complement(7078..7506)
                     /locus_tag="PKU75_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPF81921.1"
                     /translation="MKISEVVRGKGDGVITIAPDASVGDLLALLAEHRIGAVVVSGDG
                     SAVHGIVSERDVVRHLHSAGADILSGPVSAIMTSDVRTAGPDSDLDELESTMTEHRIR
                     HVPILVDGRLAAIVSIGDVVKNRISNLQAEREQLIGYIQS"
BASE COUNT         1113 a         2687 c         2764 g          878 t
ORIGIN      
        1 acagcgatga ggcgcacggg tccagcaggt cccggcatct cggtgacgag cacgcgctcg
       61 ggcatgagcg gcacgtcgag gccgtccgcc cgcgtcagtt ccggaccccg gctgacgacg
      121 agcacgccgt agggcgccct gtctcccgac gccagccggc gacgccggcc cgacccgtca
      181 tcgagggagg attggacgct gtagcccgcc ccgcggcata cggaggcaac gagatcggac
      241 ccggcacccc acccggtctc ggtcaggacg agcacctcct ccgcgcgcag ccacaggtac
      301 tccagcagcg ccttggcgcg ccccaccggg gggcgggaga gattcagcgt gaggatcgag
      361 ggcagcccgg cccccggcgc acgcccggtc aaagccgttg gctcgcagtg cgataggcca
      421 cccacccaag gcccacgccg acgacgatga gcacggccgt gaggcgtttg gggtcggtgc
      481 ggttgaggta gaacgccccg gccgcgagga agaggaggga cagcagccgg gcggggccga
      541 cgacccgcag caggatcaac ccgccgatgc cgataagggc gcccccggtg acggcgttgg
      601 gggtgctcgg ctggcgttcg ctcacggggc ccagcgtaag tccggatcgg cattggatag
      661 cgtgaggagc gagcgcgaag gagcgagaac cgatgacgga cagcaccgct ggtgagcacc
      721 cggtcgtgga gcaacgcggc ctctggttcg aggagttcga ggtcggcacg gtctaccggc
      781 accggcccgg ccgcacggtc accgaggccg acaacgtctt gttcaccacg ttgacgatga
      841 acacccagcc cctgcacctc gacgaggcgg acagcgcggc ccggccgccc ttccacgcgc
      901 gcctcgtgaa ctcgatgttc acgctgtcga cgctcgtcgg gctgtcggtc tcgcagctga
      961 cgctcggcac gattgtcgcc aacctcgggt tcagcgaaat cgctttcccc gcaccggttt
     1021 tccacgggga cacgctctat ggcgagaccc tcgtcgtcga caaacggccc tcgaagtccc
     1081 gtccgggaga ggggatggtg acgctgcggc atacggcccg caaccaggac ggcgtggtgg
     1141 tcgccacggc gacccgcgcc acgctggtgc ggatgcggcc ggaggaggag cgatgagcga
     1201 gtccacggcg gtatggacgc ccggcccggc ctggctcttc tgcccggccg accggcccga
     1261 ccgctatgcg aaggcggcgg aacggtccga tgtggcgatc atcgacctcg aggatgccgt
     1321 tgcgccgcag gacaaaccgg cggcgcggga ggcgctggcg gcggcatacg gcgggctcga
     1381 ccccgagcgg accctggtgc ggatcaaccc ggcggccacg gccgatcatg cgctcgacgt
     1441 cgacctcgtg cagcgggccg gcatccggcg ggtcatgctc gccaagaccg agtccgcagc
     1501 cgacctggcc gccttgggcg cctgcgaggt cgtcgtcctc ctcgagaccc cccgcggcgt
     1561 cgtcgccgcg cgcgagatcc tcgaggccga accggtcgtc ggcgcgatgt ggggggcgga
     1621 ggatctcgtg gccgggctcg gcggcacctc gagccgcgac gcggcgggcg cctaccgtcc
     1681 ggtggcggtg caggcccagt gcaccgccct gctcgcggcc aaggcggccg gcaaactggc
     1741 cgtcgatgcg gtgcatctgg ccatcaccga ccacgacgga ttgcgggtcg agtccgaggc
     1801 ggcggtggcg agcgggttcg acgcgaaggc gctcatccac cccacgcacg tcgacatcgt
     1861 ccgggcagcc ttccggcccg acccggccca ggtggcctgg gcagagcggg tcctcgaggg
     1921 cgagaagcaa catggcagtg gggtgttcgc gctcgacggc gccatggtgg acggccccgt
     1981 gctcgcccag gcccggcgag tcctggcccg gtcctaaagg gccgccacac cgcgccggca
     2041 tgccggccct gctgcgtcgg gctcctgcgg accccggcgc agcagcgccc cgaggctgac
     2101 gaaggccggc cgccgggact cgatcagccg cacgtcgacg acgccgggca ccgcccgccg
     2161 gatggcggca gcgagcgtgg tcgagatggt caagcccgag gcggggcagc cgtcgcaggc
     2221 gccggacaac tcgaggtcga cgacacccgc gtgcaccccg gccacccaca ccgctccccc
     2281 gtgcgcccgc acgacgtcgc cggccgggcc ggccaacacc gcggtgatct cctccaccga
     2341 cacctcggcc gctccggccc gccaggcctg cggctgctcc agtgcggcgt gcaacgcgct
     2401 gcgcacgcgc gccccctcgg cccgccagct gcgtcccggc ccgagcgtga cgagcaccgc
     2461 cccgggtaac agctgcagcg ccgtgatcgt cccgtcggcg acgagcccgg cgagcggctc
     2521 gggcacggcg gtgacggggc cgttgccggc gggggcctgg gcgacgaccc accgcacgct
     2581 ggccggatcg ccggcaacgg cctcgggatg catcgcgatc atggccacca cgctcaggtc
     2641 acggccgcgg tgatggtgcg ggcgaggaat gctgcgaccc aggccagcgt gaacatatag
     2701 ccgaaggcga gcagcggcca gcgccagctg ccgctctcgc gccgcagcac tccgatcgtc
     2761 gagacgcatt gcagcgcata gacgaagaag gccaacaagg cggcgatcgt cggcggggtg
     2821 aagagccgtt cgccggcgcg tgggccgtcg gccactcgca tcgcccgcaa cgcctgcccg
     2881 gggttctccg ggtcctccgc cgccgccacc tgccccatgg tcgcgacgaa cacctcgcgg
     2941 gccgcgaggg aggagacgac gccgacgttg atgcgccagt cgaacccgag cggcgcgaac
     3001 accgggccta cggcgcggcc gatgtccgcc gcgaagctgt tgtccaggac gtatgccgcg
     3061 ctggcggccg ggtcggcggg gtcgaccccg gcggcgcgca tgtcgtccgc gctgcgcgcc
     3121 ggcaggttga gcaggaggaa caggagcaag gtggtggcca ggatgatgtg ggtgaccttg
     3181 cgcaggaacg agcggcaggc gtcccacacg gagaagagca ctactcgcag gctggggatg
     3241 cgatatggcg gcagttccat atagaagggc agcgcctgcc cggtcctgcg gccgacccgc
     3301 gtgaacagcg cggcggtgag cagcgcggag agcgcgccca cgacatacag cgcgaacagc
     3361 accaccccac gcgccccgaa cggccccacc tgctccgacg ccgggatgag catcgacacc
     3421 agcagcacga agaccggcag ccgcgccgaa caggtcatca gcggcgccga catcatcgtc
     3481 gccagccggt cgcgcgccga gggcagggtg cgcgtggcca tgatccccgg gatcgcgcac
     3541 gccaccgacg acagcaggct gacaaaggcc cgaccctcca gcccgaaccg ggccatgaac
     3601 cggtccatga tgaacgccgc ccgagacagg tagccgacgc cttcgagcag cgagatcatc
     3661 aagaacagca gcgcgatctg gggcaggaag accagcaccc cgccgatgcc gccgagcagc
     3721 gccgtgccga cgaactcccc cgcccacccg tgcccgaggc gggccgcggc gagcgcgccg
     3781 agccagccga agaactgctc gatccagccc tgcagcggcg cggcgacggt gaagatgatc
     3841 tggaagaagg cgaacatgac ggcgaagaag atgagcccgc cgagcagcgg gtgcagcagc
     3901 acggcgtcca atttctgggt gcgccggtcc gccccggccg gccggtagtc cgccgcggcc
     3961 agcaccgagt cgatccaggc cgccccctcc gggccttcga ccggcggcgg cagcggtggc
     4021 cgtggccagg agtcgatctc ggcggcggcg tccttgaggg ccgccagccc ggccgggtcg
     4081 ccggccgtca cgggtatgac gcgcaggccc agcgcccgct gcagcttgcc gacgtcgacg
     4141 tgcccgccgc gggcggccag ttcgtcgccg aaggtcaaga taacgcagag cggctgttcg
     4201 ctcacctgca cctgcgcgag cagcggcagg ctgcgccgca gcgtcgtgac gtcgagcagc
     4261 acgagcaggc cgtcgggggt ggggctggcc gccacctccg ggtcgagcgc gtcgatgacc
     4321 acctgctcgt cgaggctgat cgggtcgagg ctgtaggtgc ccggcaggtc ttcgacgatc
     4381 agcggtatgc cgtgcgctcg cgccggcccc tcatagcggg cgaccgtcac gccggggtag
     4441 ttgccggtct tgccgtgcag gcccgtgagc gcgttgaaaa gggtcgtctt gccgcagttg
     4501 gggctgccga tcagggccag tcgcaggcca ctgagggccg aggccccacc gccttgcccg
     4561 tggtgctgat gcgcgctcat cgggcctccg agaccacgat ccggcgggcc tcgccgacgc
     4621 gcagcgcgat ctcgtagccg gcgatgtcga cgatgaccgg cccgctgaag ggtgcgcgcc
     4681 gcaccacggt cacgggggcg ccgacgacga agccgaggtc tccgagccgg cgtccgatct
     4741 cgggcgccac atcggcccgg aacccgctca ccgtcactcc ggcctccccg atcgccacgc
     4801 tgtcgagggt gcggggcacg cgcgcggcga ccggcactcg gcgtttgccg agccgactgg
     4861 ccacccggga caaatccttc acctcatcat cgcgaccgtt gccgatcagg cgaggctacc
     4921 ctgacctgct ggaccccgcc gcaccgcccg ccggctcgat gaccggacgg caacggtcac
     4981 ggcgcggtcg tgagcgactg gagaatgtcg ggggcaccgg tcagcggcca ccacagtggc
     5041 aggtcggcgg acatcccggc ccacaccgcg agcccggtcg ccgccgccag cagcaccccg
     5101 gtgacgcctt gggcgaaaac gtcgccgggg gcgagtccgc gggcgaggct gcggctgccg
     5161 cggcgcagac cggcgccgcc gggcccccac cacgacatca tgacggcgag cgccatcccg
     5221 acggcgaggg caacggcata cgtgagccgc tccgggccgg cgacgtccgc catccgcgcg
     5281 gcgagcaccg tcgcgaacac acccgccagc cccaccgcaa gcgggatgag ggcgcccgcg
     5341 agggtcgcga cgaggccgat cagcaggtgc accgggctgc tgagcaccga catcgccagg
     5401 tcgctcggac gctccccctt gtggtggcgg cgcaggacga gtgaggtgac gctgcggtcc
     5461 gccgtgcgcg cgagcaccga ccaccccagc cccaccacga gggcgagcgc cggatgaccc
     5521 gcggcaccgg cgaccagggc cgcgccgagc gccagcagca ccccgccgcg ggaggcccgc
     5581 ccgatgcgcg ggtcgccggc catcgccggt cgcggcggga gcggacgctg cgggtatgcc
     5641 gccccctgcc cctccggctg cgggtcgccc tcccacgagg acggggtgag cgccgacgcg
     5701 ccggcgaggg tgggccgctc gtccgggccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnng gtgcgggccg gccgggtgtg tcgctgcggc cgggtggcgc cgccggggct
     5881 gcggcgtgag cccgcgggtc gtcccgcggg tcgtatgccg gggctcgggt gtacgccggt
     5941 cgcggctggg gcgagccgtc gtaggccgtc ccgccggcgt atgccgtccc gcggtcgtat
     6001 gccgtcccgc cggcgtagga cggctgggcc gccggttcgc gcgcgggcgc cggggccggc
     6061 gcgacgaccc ggggcggggg cggcgccacg ggcggcaaca cggcggtggc gggtccggac
     6121 agcaactcgg tcacgggcgt tgcggcagcc gaggtgccgg gagcctcccg gcgggtcacg
     6181 acccgcagca cgtccgtgac ctgcccgccc gcggcatacc gctccagggc gcgcagcacc
     6241 tcggtgtcgg tgggccggtc ctccggatag ggcgagaggg cggcctcgag cagcggcacg
     6301 agatcggggt cgacaccggc gagatcggcc tcgccgcgca cgacccgcga caggatcgtc
     6361 tccatcgagc cgcgtccgaa cggcgggcgg ccctgggcgg cgaagcccaa ggtggccgcc
     6421 cagccccacc agtcggtgga gtcggtgacg gcggcgccct cgacgatctc gggtgacagg
     6481 tagccggggg tgcccatgac caggcccgtg cgggtcagac ggacgtcgtc ggcgacgtgg
     6541 gcgatgccga agtcgatgag gatcggctcg ccgtcgagaa gcaggatgtt gccgggtttg
     6601 acgtcgcggt ggatgacgcc ggcctcgtgg atggcgtgca gggcgccggc caggccgcgg
     6661 ccgacctgca acagggccgc cccgcgaagg gggccggcgt cgtcgacgac ctcgtccagg
     6721 cccggcccgg cgatatagcg ggtgacgata taggggcgct ccccctcgac gtcgtggtcg
     6781 atgatcgggg cgacattggg atgacggatg cggctgaggg tgtcgacctc ccgggccagc
     6841 cgcgtgcgtg ccgccgggtc cgccgcgacg tgcggacgca gcaccttgat ggccaccgcg
     6901 cgcccggccc ggtcgagcgc gaggtgcacc acgcccatac cgccctcgcc gagctgttgc
     6961 agcagccgat aaggcccgat ccgcgtgaac tcgggcagct cccccgccgg ctgctcactc
     7021 atacggacag gttaaaggac ggacctggcg cccgcggtca gcgtccccga ggtcgagtca
     7081 ggactggatg tagccgatga gctgctcgcg ctcggcctgc aggttgctga tccggttttt
     7141 gacgacgtcg ccgatgctga cgatggcggc gagccggccg tcgaccagga tcgggacgtg
     7201 ccggatgcgg tgctcggtca tcgtcgactc gagctcgtcc aggtcggagt cggggccggc
     7261 ggtgcgcaca tcggaggtca tgatcgccga gacgggaccg gagaggatgt cggcgccggc
     7321 ggagtgcagg tggcgcacga cgtcacgctc ggagacgatg ccgtggacgg cagacccgtc
     7381 gccgctgacg acgaccgcgc cgatccggtg ctcggccagg agcgccaaga ggtccccgac
     7441 gctcgcatcg ggtgcgatgg tgatgacgcc gtcgcccttg ccacgaacca cttcagagat
     7501 cttcatggca ggaccgtagt cggtccggcc cctccccgct ag
//
LOCUS       DAOPJG010000170         7317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_10826_length_7317_cov_8.528367, whole genome shotgun sequence.
ACCESSION   DAOPJG010000170 DAOPJG010000000
VERSION     DAOPJG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10826_length_7317_cov_8.528367"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..602
                     /locus_tag="PKU75_13410"
     CDS             153..602
                     /locus_tag="PKU75_13410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPF81922.1"
                     /translation="MHPPTEGTSMTGRLPTFDDALAVLAGQNFSALVGAELIAFGTGA
                     AALKIPLRADLLQQHGVAHGGVLAYAADNAITFAAGSLLGADVRTTGVSVAYIRPAAG
                     AYLVATATVVTSTSRSAVCSCEVVAFDEDDQASIVAVAQGTVRRAQS"
     gene            complement(593..2005)
                     /locus_tag="PKU75_13415"
     CDS             complement(593..2005)
                     /locus_tag="PKU75_13415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HPF81923.1"
                     /translation="MTDTAAGGLRGQRSPDLRRNQWTFGIGTLGRDAIYTLVSFYQVV
                     FLTEVLDLPDATMWWVSAVLLGVRIADAFLDPVVGAFVDSTRTRWGQFKPWMLVGGVA
                     SAVMTVLLFTDTGLRGGQLVGVYGLFMLLWGITWSAHDISYWGMLPALSVNPVERERM
                     AAVAKTFASVGQFAAVAGVPVAASAVAGVDGGDAASWRFVAIVIAVVMVALMAVTIAL
                     VRERKDIDLEGERTGIRELLRALFRNDQLLWAAAAYLLFMLGYGTTGSFGFYYFKYVY
                     GDEAVFPVFALFVGLGQIAGLATFPFVRRRWSRGQIYTWSTLAILVTYVVFYVAPLNL
                     VVLGALAFTLFFLASFIMLLMLVFQADTIEYGQWKLGRRNNAVTFALQPFINKTSAAM
                     NTWIVSATIILAGINDADTAADVTDRGVTMVKISMLLIPAVLVVLGYLVWRAKFRIDE
                     ALHAQIVRELEPELRHRPQD"
     gene            complement(2039..3725)
                     /locus_tag="PKU75_13420"
                     /pseudo
     CDS             complement(2039..3725)
                     /locus_tag="PKU75_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006213811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(3751..3921)
                     /locus_tag="PKU75_13425"
     CDS             complement(3751..3921)
                     /locus_tag="PKU75_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81924.1"
                     /translation="MYADDASAQDALTRWRTAASECDGLTVEDEADRADDRQTLAMLA
                     KGMCRFTTEGCS"
     gene            complement(3957..4718)
                     /locus_tag="PKU75_13430"
     CDS             complement(3957..4718)
                     /locus_tag="PKU75_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81925.1"
                     /translation="MSDIDTRLRELAERLRADPDVDGAEVRSIGRRRRVRRQIAMVCA
                     ATVVTVAAAFGGASLWNSPTPPAGPQPAVVPVWAERIADDPQVFRHAPGGSGLAGATA
                     TVEGTLAIAGGCLVLQGPPAESPEGTPRVLVLPVGATWTTDGGGMASLGAEGAKALPL
                     GTKITARVGISERTDRTFSDLCPGSAGYAALATPDAVVSAQPPSAADTTTPATLDTIP
                     LADGLEGAGDGSLDEPSRGLVGQASPAPWMRSPPS"
     gene            complement(4715..5227)
                     /locus_tag="PKU75_13435"
     CDS             complement(4715..5227)
                     /locus_tag="PKU75_13435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HPF81926.1"
                     /translation="MLIVVPEGGLVDAAEFDHWYAAAYPRLVAQLTALVGDRAEAQDC
                     VQEAFVRAWDRKPTLESVGEQDAWIRTTAYRLAVSRWRRLRRGRELTAQGLQTPRDPL
                     ADSETRLDLLDVLQRLPLDQRRVIVLHYFADRSVAQIAAEIGVPEGTVKSRLHRARAG
                     LAGVSEGSLR"
     gene            5425..5631
                     /locus_tag="PKU75_13440"
     CDS             5425..5631
                     /locus_tag="PKU75_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81927.1"
                     /translation="MRHSRPGCGPDAARLRVQKKDSELTLSRLAADLIGRDLSLDTRA
                     CGSAIGLTPRSLDAETVATVAACR"
     gene            complement(5647..5925)
                     /locus_tag="PKU75_13445"
     CDS             complement(5647..5925)
                     /locus_tag="PKU75_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81928.1"
                     /translation="MSEHLLVFPERDDADEVAEELRDEGFTEVRVLRQPLAGEDDAES
                     DEWAVYVREANVDDDSGAVASGLRDRFEATASERGGWYDPEPEPRYES"
     gene            6007..6597
                     /locus_tag="PKU75_13450"
     CDS             6007..6597
                     /locus_tag="PKU75_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81929.1"
                     /translation="MAPTRVAHYRTPEELAEFLPTLDATAKDRGTVKLIVRRPAVGER
                     EVLGVGRLDPAYGLQGDTWIERGSKRTSDGSSHPDMQLNVMSHPMVEFLAQDPALEPL
                     AGDQLYLDLDLSQDNLPEWTLLLFGDPHAPGAVIQVTDQPHTGCKKFVERFGPEAMRF
                     VNGKDGRPRRLRGLNARVTQAGEVRVGDTVTVRRAE"
     gene            complement(6632..>7317)
                     /locus_tag="PKU75_13455"
     CDS             complement(6632..>7317)
                     /locus_tag="PKU75_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81930.1"
                     /translation="AGLVVAALLLRLPPRVDGEPADPARLEFLPRDGIRELRRHPALV
                     LLVWALLPLIVTLESVNAVEVFLLRDVFGASAFQFGLGEAATGAGAVVGAFGAGAVHG
                     TRRRILVIVICLSLLPVAQIGQGLAPDFLVFLALGSIVGLTLGLSNALVFTLLLSILA
                     DTVRARVLAFVGGASRAMGLIALLLGGTLNATVGPRGAYVVAGVAGLVAVAVAAVRLR
                     PHLRAGAPP"
BASE COUNT         1183 a         2578 c         2523 g         1033 t
ORIGIN      
        1 gagggctgtc ctcaccttaa gcatccgagc gctcagccac ccgcccaagc agttgcacaa
       61 ttggcgggcg ccccccatcc gcgacggcac acccgtcgtg aggtttgtcc catccggccc
      121 gacgggccgg cgtggcgctg gaggatgagc acgtgcaccc tccgacggaa ggcacctcca
      181 tgaccggccg actccccaca ttcgacgatg cgttggcggt cctcgccggc cagaacttca
      241 gcgccctcgt cggggcggaa ctgattgcct tcggcaccgg cgctgccgcg ctcaagattc
      301 cgctacgcgc cgatctgctc cagcaacacg gggtcgccca tggcggcgtg ctcgcgtacg
      361 ccgcggacaa cgccatcacc ttcgctgcgg gttcgctgct cggagcggat gtccgcacga
      421 ccggcgtgag cgtggcgtac atccgcccgg ccgccggcgc ctacctggtc gccacggcca
      481 ccgttgtcac gtcgacgagc cggtcggccg tatgcagctg cgaggtggtc gcgttcgacg
      541 aggacgacca ggccagcatc gtcgcggtcg cgcaaggcac cgtgcgccgc gctcaatcct
      601 gaggtcggtg gcgcagctcg ggttcgagtt cgcggacgat ctgcgcgtgc agggcctcgt
      661 cgatgcggaa cttcgcgcgc catacgagat agcccaggac gacgaggacg gccgggatca
      721 agagcatcga gatcttcacc atcgtcacgc cacggtcggt gacgtccgcg gcggtgtccg
      781 cgtcattgat tccggcgaga atgattgtcg cagagacgat ccacgtgttc atggccgccg
      841 aggtcttgtt gatgaagggc tgcagggcga aggtgacggc gttgttgcga cggcccagct
      901 tccactggcc gtattcgatc gtgtcggcct ggaagacgag catcagcagc atgatgaagc
      961 tcgcaaggaa gaagagcgtg aatgccagtg cgcccagcac cacgaggttg agtggcgcga
     1021 cgtagaagac gacataggtg acgaggatcg ccagggtcga ccaggtgtag atctgtccgc
     1081 gcgaccagcg gcggcgcacg aacgggaagg tcgccaggcc ggcgatctgc cccagcccga
     1141 cgaacagcgc gaagacgggg aagaccgcct cgtcgccgta gacgtacttg aagtaataga
     1201 agccgaagga tccggtcgtg ccgtacccga gcatgaacag caggtatgcc gctgcggccc
     1261 agagcaattg gtcgttgcgg aagagggctc gcaggagttc gcgaatgccg gtgcgctcac
     1321 cctccaggtc gatgtccttg cgttcgcgca ccagcgcgat cgtgaccgcc atgagcgcga
     1381 ccatcacgac agcgatgacg atcgccacga agcgccagga ggccgcgtcc ccgccgtcga
     1441 cgccggccac ggccgacgcg gcgaccggca ccccggcgac ggcggcgaac tgcccgaccg
     1501 aggcgaacgt cttggccacc gcggccattc gctcccgctc cacggggttg accgacaggg
     1561 ccggcagcat cccccagtag gagatgtcgt gggcagacca ggtgatgccc cacaacagca
     1621 tgaacagccc gtacacgccg acgagttggc ccccgcgcag gccggtatcg gtgaagagga
     1681 gcaccgtcat gaccgccgaa gccactccgc cgacgagcat ccacggcttg aactgccccc
     1741 accgggtgcg ggtgctgtcg acgaatgcgc cgacgaccgg gtcgaggaag gcgtcggcaa
     1801 tccgcacgcc gagcagcacc gccgagaccc accacatcgt cgcgtcgggc aggtcgagga
     1861 cctcggtgag gaagacgacc tgatagaagc tgacgagcgt gtagatcgcg tcccggccga
     1921 gagtgccgat gccgaacgtc cactggttgc gccgtaaatc cgggctgcgc tgcccgcgca
     1981 acccgccggc ggcggtgtcg gtcatgcggc gaaccttact ggcgggtagc cgccggcgtc
     2041 actcgtcggc ggggcgttcg agataggcgt ggatgagcgg gacgacgctg gcgccctcgc
     2101 cggcggccga cgcgacccgc ttcatcgacc ccgagcggat gtcgccgacg gcgaagatcc
     2161 cgggcacgga cgtcgcgagg ctctccggcg ggcagttgtc gacccaatac tccatcggga
     2221 cgtcccgccc ggtgaggacg aatccccgct cgtcgcggca tacctccggc ggcagccacg
     2281 cgcagtgcgg ttcggcgccg agcaacagga acagtccccg cgcgtcggtc cgcctgcgtt
     2341 cgtgcgtcgc ggtgtcctcg atctcgatcc actccagctg atcctgaccg ccaccgtcga
     2401 cgactgccgc gttgccgcgc accgtgattc gcgggttgtg gtcgatctcg tcgatcagat
     2461 acctcgacat cgtcgagctg agatcgggcc ggcggatcag gatcgtcacc gaccgcgcga
     2521 agcgcgccag atgcacggcg gcctggcccg ccgagttgcc gccgccgacg acgaagacca
     2581 gctggtcctc catctcgcgg gcggcggtca tcgccgcgcc gtagttgacg ccccgcccga
     2641 cgagttcgtc gatcgccggt atgccgatgc gccggtagtc gacgccgctc gcgatgacga
     2701 cggttcgggc gcgcacgtcg ccgccctcgg tgcagacggt atgcggatcg cccccctccc
     2761 ccggtttcag cgccgtcgcc ggccagccgg tgaagaactg cgtgccgaaa cggatcgcct
     2821 gactgcgggc gcgctgcgcg agcttgccgc cggagatccc gcgcggaaag cccaggtagt
     2881 tgcggatcat cgaggacgta cctgcttgtc ccccaatggc ttccgcctcg atcgccaccg
     2941 tgctcagccc ttcgctcgcg ccgtagaccg ccgcggcgag cccgccgggc ccggccccga
     3001 cgatgaggat gtcggcgacg gtgtcgacga ggatgtcgtc cggccggccg gtgatcatcg
     3061 tggccacctc gcgcacgctg ccgggcgcga cgatgtggtc ggtcatgtag gcgcgcatga
     3121 gcgggtaggt cggctccccg tcatacagcg ccacgatctc ccggcccacc tcgctttcgg
     3181 gcgcgtagag ccggttgggc gacccgaccc ggtcgaggta gtcgcggatg gcgagcgtca
     3241 ggccaccacc gttgtcggag acgagttgga ccgagtcgac cagcggcacc ggcaccgtcg
     3301 cgccccactc ggagagcaat tcgcatacgg cagtgtggaa ttcctcgtcc cgcgcccccc
     3361 gcggcatgag gcaggaaagc atcgaacttg cccttctgca ggcccggacg caaggccggc
     3421 ccgtcctcgc ggaaccggtc ccacggcgag acgatcagcc ggcgggcggt cggcacggcc
     3481 gagcgcagca tgctgatgcc gacgagggga tgcatgtcgg gcagcatcga atccgcgacg
     3541 aacatcgcga tgtcgccgcc gcgccgcacg acccgcatcg tcgcggccat cgcgtcatcg
     3601 cccgagcgag cagtgacgat gtcatagtca cgggcatacc ggccgaactc gtcggccagg
     3661 tgttccgcgt gttccgcgga gacgatgatg atcgccggct tgctcccctg tggtgcctcg
     3721 gtcacatggg gatcgtaggc cgccttctcg tcaggagcag ccctccgtcg tgaatcggca
     3781 catgcccttg gccagcatcg cgagggtctg ccggtcgtcg gcgcggtcgg cctcgtcttc
     3841 cacggtgagc ccgtcgcact cgctcgcggc cgtgcgccac ctggtgaggg cgtcctgggc
     3901 tgaggcatcg tccgcgtaca tccgggccac tcgaaagacc tgccccgagg agctggtcag
     3961 gacggcgggg atcgcatcca cggcgccgga ctcgcctggc cgacgaggcc ccgcgacggt
     4021 tcgtcgaggg aaccgtcgcc ggccccttcc agaccgtccg cgagggggat ggtgtcgagg
     4081 gtcgcggggg tcgtggtgtc ggcggcgctc ggcggctggg cggagacgac ggcgtcgggc
     4141 gtcgcgaggg cggcataccc ggcgcttccc gggcaaaggt cggagaaggt cctgtccgtg
     4201 cgctccgaaa taccaactcg cgcagtgatt ttcgtgccca gcgggagggc tttggcgccc
     4261 tcggctccaa gactcgccat ccccccaccg tccgttgtcc aggtggcgcc gacggggagg
     4321 acgaggactc ggggcgtgcc ttcgggggac tccgctggcg ggccctggag gaccaggcac
     4381 ccaccggcga tcgccaatgt gccctccacc gtcgccgtgg cgccggccag tccggacccc
     4441 ccgggggcat gccggaacac ctgcgggtcg tcggcgatgc gctccgccca cacgggcacc
     4501 acggccggct gcgggccggc gggtggcgtg ggcgagttcc acaaggacgc accgccgaag
     4561 gccgcggcga cggtcacgac cgtggcggca cagaccatcg cgatctggcg gcgcacgcga
     4621 cgccgacgac cgatgctgcg cacctctgcg ccgtcgacgt cgggatccgc gcgcagacgc
     4681 tcggccaact cgcgcaagcg ggtgtcgata tcgctcatcg aagactcccc tcggatacgc
     4741 cggcgaggcc ggcgcgagca cggtgcagcc gggacttgac ggtcccttcg ggaacgccga
     4801 tctccgccgc gatctgagcg accgatctgt cggcgaaata gtgcaggacg atgacgcgcc
     4861 gctgatcgag gggcagccgc tgcaggacgt ccagcagatc gagccgagtc tcactgtccg
     4921 ccaagggatc ccgcggggtt tgcagtcctt gtgcggtcag ttcccggccg cgccggagcc
     4981 ggcgccagcg gctgacggcc agccggtatg ccgtggtgcg gatccacgcg tcctgctcgc
     5041 cgacgctctc gagagtgggc ttcctgtccc aggcgcgcac gaaggcctcc tggacacaat
     5101 cttgcgcctc ggcgcgatcg ccgacgagcg cggtgagctg ggcgaccaac cgggggtatg
     5161 ccgcggcgta ccaatgatcg aactcagcag cgtccaccag gcctccctcc ggcaccacga
     5221 tcagcatctg cgatcatcac aagaacgcac ccgggcgacc ggccggttgc atgcgggcgc
     5281 gacttcttcg ccaggtgctc gctgcgagaa tgaccaaccg cccccgcatc tcgaaaggcc
     5341 cccatgcgca tcgtcgtcct cggctcgggc gcgtcatcct cgatgcgctc ggccatccgc
     5401 gccgtccggt ggtgtcgatg cccaatgcgg cactcacggc cgggttgcgg gcccgacgcc
     5461 gcacggctgc gggtgcagaa gaaggactcg gaactgaccc tttcccgact ggccgccgac
     5521 ctcatcgggc gcgacctgag cctggacacg cgcgcctgcg gcagcgcgat cggcctgacc
     5581 cctcgatcgc tggacgcgga gaccgtcgcg acggtcgccg cctgccgctg aaccggcgcg
     5641 ggcgtctcag ctctcgtagc gcggctcggg ctcggggtcg taccagccac cgcgctcgct
     5701 cgcggtcgcc tcgaagcggt cgcgcaggcc ggacgcgacg gcgccgctgt cgtcatcgac
     5761 attggcctcg cggacataga ccgcccactc gtcggactcg gcgtcgtcct caccggccag
     5821 cggttgccga aggacgcgga cctcggtgaa gccctcgtcg cgcagttcct cggcgacctc
     5881 gtcggcgtcg tcacgctcgg ggaagaccag caggtgctcg ctcacgcggc cactgtatga
     5941 cgggagaccg cgcccgaggc gtgaaaccgg ctgcgctgct cgaccgccgg ctgccatgct
     6001 ctggcgatgg ctcccacccg cgtcgctcac taccggaccc cggaggaact ggctgaattc
     6061 ctgcccacgc tcgacgccac cgccaaggat cggggcacgg tgaagctcat cgtgcggcgc
     6121 ccagcggtgg gcgagcgcga ggtgctcggc gtcggaaggc tggacccggc atacggactg
     6181 cagggcgaca cctggatcga acgcggatcg aagcggacca gtgacgggtc ttcccacccc
     6241 gacatgcaac tgaacgtcat gagccacccg atggtggagt tcctcgccca ggatcccgcc
     6301 ctggaaccgc tcgccggcga ccagctctat ctggatctcg atctctccca agacaatctg
     6361 cccgagtgga cgctgctgct gttcggtgat ccgcacgcac caggcgcggt catccaggtg
     6421 acggatcagc cgcataccgg ctgcaagaag ttcgtcgagc ggttcgggcc ggaggccatg
     6481 cgcttcgtca acggcaagga cggccggccc cgccggcttc gcgggctcaa cgcccgggtc
     6541 acccaggccg gagaggtgcg cgtcggcgac acggtgaccg tgcgccgggc cgagtgacac
     6601 ctgtccgtct ggacgacatc gctcagccct cctagggtgg cgcgcccgca cgcaggtgcg
     6661 gacgcagccg gacggcggcg accgccacgg ccaccaggcc ggcgaccccg gccacgacat
     6721 aggcgccgcg gggccccaca gtcgcgttga gcgtgcctcc gagcagcagc gcgatcaggc
     6781 ccatcgcgcg cgatgcaccg ccgacgaagg cgaggacgcg cgcccgcacg gtgtccgcca
     6841 ggatgctcag gagcagggtg aagacgaggg cattgctcag gcccagcgtc aggccgacga
     6901 tcgacccgag ggcaaggaag acgaggaagt cgggcgccag gccttgcccg atctgcgcca
     6961 ccggcaggag cgacaggcat atcacgatca cgaggatccg gcgtctcgtg ccgtgcaccg
     7021 cgccagcccc gaaagccccc acgaccgctc cggccccggt ggccgcctcc cccaggccga
     7081 attgaaaggc gctggcgccg aagacatctc gtaagaggaa gacctcgacg gcgttcacgg
     7141 attccagggt gacgatgagc ggcaacagcg cccacacgag gagcacgagc gccgggtgac
     7201 gacgcagttc gcgaatgccg tcacgtggca ggaactccag ccgggcggga tctgccggct
     7261 ctccgtcgac ccgcggcggc aggcggagaa ggagcgcggc caccacgagg ccggcga
//
LOCUS       DAOPJG010000171         7133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_11174_length_7133_cov_6.423283, whole genome shotgun sequence.
ACCESSION   DAOPJG010000171 DAOPJG010000000
VERSION     DAOPJG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11174_length_7133_cov_6.423283"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PKU75_13460"
     CDS             complement(<1..164)
                     /locus_tag="PKU75_13460"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF81931.1"
                     /translation="MLHLTTGLPGTGKTTLAREIEARDNALRLTPDEWMNPLFGHHDA
                     DGKRDVLEGRL"
     gene            269..1765
                     /locus_tag="PKU75_13465"
     CDS             269..1765
                     /locus_tag="PKU75_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPF81932.1"
                     /translation="MTNLVTDLVSTATKQPDATFIAMNGNHLPYGAVHQLAAKLAGEL
                     RTAGIEPGDRVALILPNVPAFPVAFYATLLAGGIVVPMNPLLKAGEIDFFFSNSGAKI
                     AFVWPDFVEEVTKGAANSGTRVIPCDPMGPVAGSLEPGEPIAHPMERADDDTAVILYT
                     SGTTGRPKGAELTHSNIHLNATRSAVDIAQISPDDVVMGCLPLFHVFGLVVGMHAAVI
                     AGASLALIPRFDPSDAIKTMANEKVTVMLGVPTMYAAILHHPESDSFDASNLRTCCSG
                     GSAMPAEVQRAFEEKFDAQILEGYGLSETSPVASFNMPDRPTKSGTIGVAIPGCEMKL
                     LDEDGKDVGVGGVGEIAIRGDNVMKGYWDNPEATAQAIPDGWFRTGDMATVDDEGYYT
                     IVDRKKDMILRGGMNVYPREVEELIYTHPDVLEVAVVAVPDELLGENVGAAIVLRPGV
                     TTSVEDVQAWTKERVAAYKYPRTVWQVEELPKGPTGKILRREVHPPTA"
     gene            complement(1766..2263)
                     /locus_tag="PKU75_13470"
     CDS             complement(1766..2263)
                     /locus_tag="PKU75_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPF81933.1"
                     /translation="MAMKDYNTIIVGTDGSALAAPTVARAAWLATRDDADLVIICAFS
                     ELPRRVEAKNPATIGGDSRAGQVIGKAAASAAVAAAVAIAQAEGATIAAAQLVDGDPA
                     KALVQAAADRGADLIVLGAVQDRSLADRLLGTTAEEVTKRAACDVLIVRPAGGEAELE
                     VPESG"
     gene            complement(2281..3690)
                     /locus_tag="PKU75_13475"
     CDS             complement(2281..3690)
                     /locus_tag="PKU75_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81934.1"
                     /translation="MSSDAVATQGEGNLQHTTIVRQKPLMTMPQILIMNFGFFGIQYS
                     FGMQQTAVNPIFQKLGAEGHALPLLNLAGPITGLLIQPMIGAMSDRTWSPRWGRRKPF
                     FLIGALGCSICLFLFPFVSAIWMAVLLLWLLDASNNTAMEPYRAFIADKLPPSQLAKG
                     FLAQSFFTGFGITLANISLFVFQKIISGGTDAGIPYWVFGSFMLGAVCSIGSVLISVL
                     RTPEIPPTPEELAAIQAKKAEGGGFGKAIAEIKEAIVEMPTELRKLALVYLFQWYALA
                     CYWQFVTASLAKSVWNTTDTESAAFDEAVGWTGLVNGWYNIVTFSVAFALVAFARRRG
                     AKMVHSACLLLAAIGLVIFPHLDNKYLVFIPIIGFGIAWASIMGVPYIMAVRMIPSTR
                     YGVYMGIINMMIVIPMLIQSLTFGTIYSSVLGDNPNNAIMFAGVFLAIAALVMQWIKE
                     PPIVRDVDDVGAMPMAGGH"
     gene            complement(3687..4178)
                     /locus_tag="PKU75_13480"
     CDS             complement(3687..4178)
                     /locus_tag="PKU75_13480"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPF81935.1"
                     /translation="MTANVMVVVERQFDFPRQEVFRAWTDPQQLKRWRGSPGWHVEDE
                     TVGGDLKLGGRHTHVKIRDNDPTTRVVTDGVYSEYFEPDVFVSRERITGDPGIDPNVP
                     LELRVEFTKYGREGKGTLVRIIQGPYDPDVAGWHGEGWEKELTRLQNFLAGTGEYANG
                     GRA"
     gene            complement(4278..5234)
                     /locus_tag="PKU75_13485"
     CDS             complement(4278..5234)
                     /locus_tag="PKU75_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPF81936.1"
                     /translation="MTQSRIRWQAKFIALALIWGSSFLFMKVGLAALHPIQIAAGRIF
                     AAALILAILLRATGSHLPRERRVWGHLAVSSVFLTALPFTGFVVGETRVSSALAGIGN
                     ATTPIAAVLFALALLPSERLSGRKLAAILVGFVGVVIIAEPWNAAGRPDPLGFLIVVL
                     AGACYGLGWTYNRRFLAHADLGGLSQPAALLLCGSALMVPVTLVWWLIARENFPRPWS
                     LAPGAHTADHPVWLALLCVAALGFVGTGIAYVLQYDVVRAAGAVVSTTVTYLIPVVSV
                     LLGVFVLGEHLGTAQVLGFAVVLACAFVINSAPRRRRPGQAS"
     gene            complement(5231..6220)
                     /locus_tag="PKU75_13490"
     CDS             complement(5231..6220)
                     /locus_tag="PKU75_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPF81937.1"
                     /translation="MTRATGIGSWPGTEAREAVITVRDLLSAADGLGIPYLPETPARG
                     PGADIIGRAAGLLVDLPVDLQPSGWRLVERPGRDAARTAALHRQDLDELAEAYDGYAG
                     PLKIQVAGPWTLAASIELTRGERSIVDPGATRDVISSLAEGISRYAADVRRLVPGADV
                     ILQLDEPSLPAVLAGRLPTASGYGRVRAIEAPVAGQGLRDLIGRQEVPVVIHCCDDQA
                     PVPLLRTVGAAAVAVDVLTASPARWESLAATLEAGVGLYAGCLPADAPAVVATARRLI
                     SDAFERLGLAAERLRDVTITPTCGLASSSVAQARHTHRAAVDVARELTEEEHA"
     gene            complement(6267..6785)
                     /locus_tag="PKU75_13495"
     CDS             complement(6267..6785)
                     /locus_tag="PKU75_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPF81938.1"
                     /translation="MLSEKDFDDLIESANPVMAVVTTVAEGVRAGCVVGFHGQSSVEP
                     RHYCVWLSKANHTYRTALRAEHLAVHLLTRDDMEIATHFGTTTGEDGDKFTTLSVSDG
                     PGGVPLLEALPNRFVGRRRAMLDLGGDHACVELVVDLAQRAGAFDALRLRDVVHLRPG
                     HEAEERAVSPER"
     gene            complement(6826..>7133)
                     /locus_tag="PKU75_13500"
     CDS             complement(6826..>7133)
                     /locus_tag="PKU75_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPF81939.1"
                     /translation="QTKSDVLQRITARIIAGRNDEVRPSTAKEESATIGLAMAITPDN
                     WIMKQRSGDPGRPEGPVDVWCGVVPMTVTAGVPVAAPWSVGPVPPSVTNFVRAHPAP"
BASE COUNT         1184 a         2404 c         2506 g         1039 t
ORIGIN      
        1 agccggcctt cgaggacatc ccgcttgcca tcggcgtcgt ggtggccgaa gagggggttc
       61 atccactcgt ccggggtcag gcgaagagcg ttgtcgcgcg cctcgatctc ccgcgcgagg
      121 gtcgtcttgc cggtcccggg caggccggtc gtcagatgca gcaccggaga acgagacaac
      181 actcacccac cctgccaccg acgcgcgcgg acgttgcaca ataacgccaa ccacctcccg
      241 ggaccggacc ctcgaaagtg agaaaacgat gaccaacctc gtcaccgacc tcgtcagcac
      301 ggcgaccaag cagccggacg cgacattcat cgcgatgaac ggcaaccacc tgccctacgg
      361 cgcggtccac cagctggcgg cgaaactcgc cggcgaactg cgcaccgcag ggatcgagcc
      421 cggcgatcgg gtggcgctga tcctgccgaa cgtgccggcc ttcccggtgg ccttctatgc
      481 cacgctgctc gccggcggga tcgtcgtgcc gatgaacccg ctgctcaagg ccggggagat
      541 cgacttcttc ttcagcaact ccggcgccaa gatcgccttc gtctggccgg atttcgtcga
      601 ggaggtgacc aagggggcgg cgaacagcgg cacgcgcgtc ataccgtgcg accccatggg
      661 gccggtggcc gggtcgctgg agccgggcga gccgatcgcg caccccatgg agcgcgcgga
      721 cgacgacacc gcggtcatcc tctacacctc cggcacgacc ggccggccca agggtgcgga
      781 gctgacgcac agcaatatcc acctcaacgc cacgcgcagc gccgtcgaca tcgcccagat
      841 cagcccggac gacgtggtga tgggctgtct gccgctcttc cacgtcttcg gcctcgtcgt
      901 cggcatgcac gccgcggtca tcgcgggtgc ctcgctcgcg ctgatccccc gcttcgaccc
      961 ctccgacgcg atcaagacga tggccaacga gaaggtcacc gtcatgctcg gcgtgccgac
     1021 gatgtatgcc gcgatcctgc accaccccga gagcgacagc ttcgacgcct ccaacctgcg
     1081 cacctgttgc tccggcggct cggcgatgcc ggcggaggtg cagcgggcgt tcgaggagaa
     1141 gttcgacgcc cagatcctcg agggttacgg cctgtcggag acctccccgg tggcctcctt
     1201 caatatgccg gaccggccca cgaagtccgg caccatcggg gtcgccatcc ccgggtgcga
     1261 gatgaagctc ctcgacgagg acggcaagga cgtcggcgtc ggcggggtcg gcgagatcgc
     1321 gatccgcggc gacaacgtca tgaagggcta ctgggacaac ccggaggcga ccgcgcaggc
     1381 catccccgac ggctggttcc gcaccggcga catggccacc gtggacgacg agggctacta
     1441 cacgatcgtc gaccgcaaga aggacatgat cctgcgcggc gggatgaacg tctatccgcg
     1501 cgaggtcgag gagctcatct acacccaccc cgacgtgctc gaggtcgccg tcgtcgcggt
     1561 gccggacgag ctgctcggcg agaacgtcgg tgccgcgatc gtgctgcgcc ctggcgtcac
     1621 cacgagtgtc gaggacgtcc aggcgtggac caaggagcgc gtcgcggcct acaaataccc
     1681 gcgaaccgtc tggcaggtcg aggaactccc caagggtccc acgggcaaga tcttgcgccg
     1741 cgaggtccac cccccgacgg cctgatcacc cgctctcggg gacctccagc tccgcctcgc
     1801 caccggcggg ccggacgatg aggacatcgc aggcggcccg cttggtgacc tcctcggccg
     1861 tggtgccgag gaggcggtcc gcgagtgagc ggtcctggac ggccccgagc acgatcaggt
     1921 cagcgccgcg gtcggcggcc gcttggacca gtgccttggc cgggtcaccg tcgaccagct
     1981 gggccgcggc gatcgtcgcc ccctccgcct gcgctattgc caccgcggcg gcgacggcag
     2041 cgctggcggc cgctttgccg atgacctgcc cggccctgct gtcaccgccg atcgtggccg
     2101 ggttcttcgc ctcgacccgc cggggcaact cggagaaggc gcagatgatc accaggtcgg
     2161 cgtcatcgcg ggtcgccagc caggcggccc gcgccacggt gggggccgcg agtgccgacc
     2221 cgtcggtgcc gacgatgatc gtgttgtagt ccttcatggc catctggggc tcaactcctc
     2281 tcagtggccg ccggccatcg gcatggcccc gacgtcgtcg acatcacgca cgatcggcgg
     2341 ttccttgatc cactgcatga ccagcgcggc gatggccagg aagacgccgg cgaacatgat
     2401 ggcgttgttg gggttgtcgc cgaggacgct ggaatagatg gtgccgaagg tgagcgactg
     2461 gatgagcatc gggatgacga tcatcatgtt gatgatgccc atgtagacgc cgtaccgcgt
     2521 cgagggaatc atgcgcacgg ccatgatgta gggcacgccc atgatcgagg cccaggcgat
     2581 gccgaacccg atgatcggga tgaacaccag atatttgttg tcgaggtgcg ggaagatcac
     2641 caggccgatc gcggcgagga gcaggcaggc gctgtgcacc atcttcgccc cgcgccggcg
     2701 ggcgaaggcg acgagcgcga aggccacgct gaaggtgacg atgttgtacc agccgttgac
     2761 gaggccggtc cagccgactg cttcgtcgaa ggccgccgat tcggtgtcgg tggtgttcca
     2821 caccgacttg gccagcgagg ccgtgacgaa ctgccaatag caggccagtg cataccactg
     2881 gaagagatag accagggcga gcttgcgcag ctcggtcggc atctcgacga tggcctcctt
     2941 gatctcggcg atggccttgc cgaagccgcc gccctcggcc ttcttggcct ggatggccgc
     3001 caattcctct ggggtgggcg ggatctcggg tgtccgcagc accgatatca acacgctgcc
     3061 gatcgagcag acggcgccca gcatgaacga gccgaagacc cagtagggaa tgccggcgtc
     3121 ggtgccgccg gagatgatct tctggaagac gaagagcgag atgttcgcca gcgtgatgcc
     3181 gaagccggtg aagaaggact gcgcgaggaa gcccttggcg agctggctcg gggggagctt
     3241 gtcggcgatg aacgcgcggt agggctccat cgcggtgttg ttgctcgcgt ccagcagcca
     3301 cagcagcagg acggccatcc agatcgccga gacgaagggg aagaggaaga ggcagatgct
     3361 gcagccgagg gcgccgatga ggaagaacgg cttgcgccgg ccccaccgcg ggctccacgt
     3421 ccggtccgac atggccccga tcatcggctg gatcagcagg ccggtgatcg ggccggccag
     3481 gttgagcagc ggcagcgcgt gcccctcggc cccgagcttc tggaagatcg ggttcaccgc
     3541 ggtctgctgc ataccgaagc tgtactggat gccgaagaag ccgaagttca tgatgaggat
     3601 ctgcggcatc gtcatcagcg gcttctgccg cacgatggtg gtgtgctgca ggttgccctc
     3661 gccctgggtg gcgaccgcgt cggaactcat gcccggcccc cgttcgcgta ctcgccggtg
     3721 ccggcgagga agttctgcag ccgggtcaac tccttctccc agccctcccc gtgccagccg
     3781 gcgacatcgg ggtcgtacgg gccctggatg atgcgcacga gcgtgccttt gccctcgcgg
     3841 ccgtacttgg tgaattcgac acgcaactcg agcgggacgt tggggtcgat gccggggtcg
     3901 ccggtgatcc gttcgcggga gacgaagacg tcgggctcga aatactccga atagacgccg
     3961 tcggtgacga cacgcgtcgt ggggtcgttg tcgcggatct tgacgtgcgt atgccgcccg
     4021 cccagcttga ggtcgccgcc gaccgtctca tcctcgacgt gccagccggg tgagccacgc
     4081 cagcgcttga gctgttgcgg gtcggtccag gcccggaaaa cttcctgtcg tggaaagtcg
     4141 aactgccgct cgacgaccac catgacgttg gctgtcatta gacctcgatc cccatcgact
     4201 gccgtgcacc tgcacggatg gcgtcagcca tcatcgcacg accagcgcgc gaggtctacc
     4261 ggaagattgg cggtcggtca ggacgcttgg cccggacggc gccgacgcgg tgcggagttg
     4321 atgacgaagg cgcaggccag caccactgcg aagccgagaa cctgtgcggt tcctaggtgt
     4381 tcgcccagaa cgaagacgcc gagcagcacc gagacgaccg ggatcagata ggtcacggtc
     4441 gtcgagacca cggcgccggc cgcccgcacg acgtcgtact gcagcacgta ggcgatgccc
     4501 gtgccgacga agccgagagc ggccacgcac agcagcgcca gccagaccgg atggtcggcc
     4561 gtgtgcgccc ccggagcgag ggaccagggc cgcgggaaat tctctcgtgc gatcagccac
     4621 cacacgaggg tgaccgggac catgagcgcc gacccgcaca ggagcagcgc ggcgggctgc
     4681 gagagtccac ccaagtcggc gtgagcgagg aagcgccggt tgtaggtcca gccgagcccg
     4741 tagcacgcgc ccgcgagcac gacgatgagg aagccgagcg ggtccggtcg cccggcggca
     4801 ttccacggct cggcgatgat gacgacgccc acgaacccga cgaggatcgc ggcgagcttg
     4861 cgcccggaca accgctccga cggcagcagc gcgagcgcga agagcacggc cgcgatcggc
     4921 gtcgtcgcat tgccgattcc ggccagagcg gaggagaccc gggtctcgcc cacgacgaat
     4981 ccggtgaacg gcagcgcggt caagaagacc gaggagacag ccaggtgacc ccagacacgc
     5041 cgttccctcg gcaggtggga accggtggcc cgcaacagga tcgccaggat gagcgcggcg
     5101 gcgaagatgc gcccggcggc gatctggatc gggtgcagcg cggccagccc gaccttcatg
     5161 aagaggaagc tcgagcccca gatcagcgcc agggcgatga acttcgcctg ccaccgtatt
     5221 cggctctggg tcacgcgtgt tcctcctcgg tcagttcgcg ggccacgtcc accgcggcgc
     5281 gatgggtatg ccgtgcctgc gccaccgagg aagacgccag gccgcaggtg ggtgtgatgg
     5341 tcacatcgcg aagacgttct gcggccaggc cgagccgttc gaacgcgtcg ctgatcaggc
     5401 ggcgggccgt cgcgacgacc gccggtgcgt cggcggggag gcacccggca tacagcccga
     5461 cacctgcctc gagggtggcc gcgagcgact cccaccgggc cgggctcgcg gtgagcacgt
     5521 cgaccgctac ggcggcggcg ccgacggtgc ggagcaacgg cacgggtgcc tggtcgtcgc
     5581 agcagtggat gacgacgggc acctcttgcc ggccgatgag gtcacgcaat ccctgcccgg
     5641 ccaccggcgc ctcgattgcg cggacccgtc catacccgct ggcggtcgga agccgcccgg
     5701 cgagcaccgc cggcagcgac ggctcgtcga gttgcaggat gacatcggcg ccagggacga
     5761 ggcggcgcac gtccgcggca taccgcgaga tgccctcggc gagagaggaa atgacgtcgc
     5821 gcgtggcgcc ggggtcgacg atggagcgtt cgccgcgggt cagttcgatg gatgcggcga
     5881 gggtccacgg gccggcgacc tggatcttga gcggacccgc ataaccgtcg taggcctcgg
     5941 ccaactcgtc caggtcctgc cggtgcagag cggcggtgcg agcggcgtcc cggcccggac
     6001 gctcgacgag gcgccacccg ctgggctgga gatcgacggg caggtccacg agcaggcctg
     6061 ccgcccggcc gatgatgtcg gccccggggc cgcgcgcggg tgtctcgggt aggtagggga
     6121 tcccgagccc gtcggccgcg gacagcaggt cacgcaccgt gatcaccgcc tcgcgggcct
     6181 cggtgccggg ccaggagccg atgccggtcg cgcgggtcac cgggccatca tccccgagcc
     6241 gcccgcgcgc cgcgaatcgg ctgcccttag cgttcggggc tgaccgctcg ctcttcggcc
     6301 tcgtgtcccg gccgcaggtg caccacgtcc cgcagccgca gggcgtcgaa agcgccggcc
     6361 cgctgggcaa ggtcgacgac gagttcgacg caggcgtggt ccccgcccag gtcgagcatg
     6421 gcgcggcggc gtccgacgaa acggttgggc agcgcctcga ggagcgggac gccgccgggt
     6481 ccgtcgctga cggagagggt ggtgaacttg tcgccgtcct cgcccgtcgt cgtgccgaag
     6541 tgggtcgcga tctccatatc gtcccgggtg agcaggtgga ccgcgaggtg ctcggcccgc
     6601 aacgctgtcc gataggtgtg gttggccttg gacagccaca cgcaatagtg gcgcggctcg
     6661 acgctggact ggccgtggaa gccgacgacg cagcccgcac gcactccctc ggcgaccgtg
     6721 gtgacgacgg ccatgaccgg gttcgccgac tcgatcagat cgtcgaagtc cttctcgctc
     6781 agcatgatcc gcctcgcctt ctctggtata tcgcggccgc cgtggtcacg gtgctgggtg
     6841 ggcgcgcacg aagttcgtga ccgagggcgg gacggggccg acggaccacg gcgcggcgac
     6901 gggcacccct gccgtgacgg tcatcgggac gacaccgcac cagacgtcga cgggaccctc
     6961 cggccggccc gggtcacccg agcgctgttt catgatccag ttgtcgggtg tgatggccat
     7021 ggccaggccg atcgtcgccg actcctcctt ggcggtcgac ggtcggacct cgtcgttgcg
     7081 gccggcgatg atccgggccg tgatgcgctg caggacatcg ctcttcgtct gtc
//
LOCUS       DAOPJG010000172         7053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_11345_length_7053_cov_5.814804, whole genome shotgun sequence.
ACCESSION   DAOPJG010000172 DAOPJG010000000
VERSION     DAOPJG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11345_length_7053_cov_5.814804"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2374)
                     /locus_tag="PKU75_13505"
     CDS             complement(<1..2374)
                     /locus_tag="PKU75_13505"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPF81940.1"
                     /translation="MASVSARCGEEGEVRRPEVDQSRASVAGLALLALVLGYFAAAHP
                     GVKTAELRLNDGGVWVTNTALRLVGHLNYPSRTLDGGLSAFGDEVDVTQEARSILVAD
                     PTSGVAQSVDTARLVYGVKGEVPSGFALAHGGDTVAIVDSAGGRVWVMSLAQIGSFSP
                     EGEPTVEGVRGVRVVVSRSGVVHAVAGDGGLTTVAPATGGWSVADVGRLPDYPGSDGI
                     VLTLVGEEVVATDAAAKRMWTKDRALALPEAGLVLQQPGPPANSVALSGPSSLLAIPL
                     DGSAPVRASVQRGKPAAPVVVGGCTYAAWSGSGAYIRDCAGTTNDLSRTVRRLAKITS
                     LVFRVNRDVVVINDTANGDIVLPNADFTTVTNWKDIEAQIESKDKGDEDSAETIDEVT
                     TGKRSKNNHPPRALDDTFGVRSGRATKLPVLMNDSDPDGDILTAAVPKPGNLGPISTV
                     RGGEALQITTNAESGRETIPYIVSDGRGGEDRASVTIEVHPDTVNEAPEQRRFPSVQV
                     AERHEVSYNVLPDWLDPDGDDAMLVGVAEEPGLQVRTRPDGLITIRDLGIKGPGTISL
                     KVTVGDGKDVADGTVKIVVRSKDKNNPPVANADHARVPVGTDVVVKPLVNDTDAEGDE
                     LRLSAVGAAADARISKDLTAGTFSVSSPKPGTIYVPYEVTDGPDSAQGVVRVDVVEVE
                     KKAKPVTDNDLGLLPDGGAVLVDVLANDDDPNGGVLVLRSAAATPDLGVAVEVIDHHL
                     LRVTAPSGLTRPVSFPYDVSNGTGSATGQVTVIPLPPVAQPEPPAAKDDPA"
     gene            complement(2459..4780)
                     /locus_tag="PKU75_13510"
     CDS             complement(2459..4780)
                     /locus_tag="PKU75_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPF81941.1"
                     /translation="MSSATLTTATRSAAHAPARAWWRAHPFADAIGLSASLAAAAMAW
                     RPAYDSFRYLLMIVVAALVGFGCARIGIIVAGRASAPGRATAQALAATLAAVAAYLAL
                     SPVTVLGVPTDFRRMAPVPVTGWKDMLSSLPPVDVGTTLGGLPVSLGLAAGVIGGLLA
                     HRPRAWMPVTVPITVLGLMAFLGTTNRAWAVSAAVLTAAATACWGAVRRRRVRRVAGT
                     GGARISQAAIAVALLGVSALGTGIAIPHLPGVESRPRLLARTLVEPPFDVSELVSPLV
                     GFRRYGAGLNQLWGQELLTVTGAARDARLRFAVLDSYDGTTWSAGTMTATGGVFHRVG
                     GAFPEPETPGGEPQQISVTIGDAYAAIPELASWVPGLGTPTRAHFAGPHGGDAATSLR
                     FNPATGQLLVPSRLASGTVIEQTTYVVREVPKTGIEPDPLAAIDQAGGAFVAPWVAEW
                     AGDAEQPWARLAAIGRHLETTGAYSDGTRQGEEDYWPGHGQGRLLRLLAGPQPVGSDE
                     QYAAAFALLAQQTGIAARVVMGAIVPGDGHVRGQDVHAWVEVREASGEWLAVPNSLFM
                     PQRSKRPKQTDYEDAPPAEQAVVPAPVGRRPPGSVDSLIEGVPPLQRPRPSGRPTEAN
                     PGLPGLPAESLPGWVTPVARYAGPPLAMVGGTGLLLAGLRAGRRSRRRSRGDASRRLA
                     AGWRDLIDHARDLGHHVEPGHTRLEQAAVIGHADLAGRANRAVFDSHDPSPDAVSSYW
                     ADIAKARKSMSRGASWKARLSRPWHLRGLLARE"
     gene            complement(4777..5991)
                     /locus_tag="PKU75_13515"
     CDS             complement(4777..5991)
                     /locus_tag="PKU75_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPF81942.1"
                     /translation="MSQRRHTVPSLGEHVDPVLDAVSPLGWAVLATAVVAWVVGWVGG
                     WIELSYLAGMLTLLFALSCLLTIGRTQLDVRVRVQPTRVRVGEAAAAEVRVRNDGRLP
                     VPAMPLELGIGESAVAVQLPAIRPTESFDDVVILPTGRRGVYPIGPATTLRGDPYGLV
                     RRAVRRTDPVELIVHPHTVYVDGFTSGILRDLEGRTTEDVSSSDLAFHGLREYVPGDD
                     RRHIHWLSTAKRSSAAGQSDLMVRQFLDTRRTSVGVVADCAATSYADPDHFETVLSAA
                     ASIAQRAAHDKIELSVAAGSFVLRGPRGNAALDLFARARLGPEQLDAATGRLVQFAPT
                     LSSVALVIGEHADLARVARARTSLAPGVNVHVLRFEPGADVRLQHASGMTLVTFGSLE
                     DLPAALAGRRRA"
     gene            complement(5963..6928)
                     /locus_tag="PKU75_13520"
     CDS             complement(5963..6928)
                     /locus_tag="PKU75_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPF81943.1"
                     /translation="MTLTDQDATHFAGAFDQMVANIEKAVRGKTYVTRLALTCLIAGG
                     HLLVEDNPGTGKTQLAKALANTIEGTHSRIQFTPDLLPTDITGVSVYDQSTQRFAFRP
                     GPVFASIVLADEINRASPKTQSALLEVMEEGRVTVDGQPHGVGSPFMVIATQNPIEQA
                     GTYRLPEAQLDRFLMKTSLGYPDHDTTVELLADAENRDRSGAIEPILRADSVRAMSAQ
                     AGLVHVDPSILSFIALIGEESRRHSHVSLGLSVRGGLAFVRSAKTWALSQGRPYVTPD
                     DVKGLAQPVLAHRILLGDEAQFSGVRVGDVIDELIDSVPAPAYRP"
BASE COUNT         1079 a         2382 c         2519 g         1073 t
ORIGIN      
        1 cggccgggtc gtccttcgcg gccggcggtt cgggttgcgc gaccggcgga agcgggatga
       61 cggtcacttg cccggtggca gagcctgtgc cattggacac gtcgtacggg aaactcacgg
      121 ggcgagtcag accggatggg gcggtcacgc gaagcaagtg atggtcgatg acctcgaccg
      181 cgacgcccag atccggcgtg gctgccgcag accggagcac gagcacgccc ccgttggggt
      241 cgtcgtcatt ggccagcaca tccacgagaa ccgcaccgcc gtccggcagc agtccgaggt
      301 cattgtcggt gaccggcttg gctttcttct ccacttcgac gacatcgaca cggacgacgc
      361 cctgggcgct gtcggggccg tccgtcacct cgtacggcac gtagatcgtg cccggtttgg
      421 gggaactgac ggagaacgtc ccggcggtga gatccttgct gatccgcgcg tccgcggcgg
      481 cgccgacggc gctcagacgc aattcgtcgc cttcggcgtc ggtgtcgttg acgagcggtt
      541 tgacgacgac gtccgtgccg acggggaccc gtgcgtgatc ggcattcgcg accggcggat
      601 tgttcttgtc cttgctccgg acgacgatct tgaccgtccc gtcggcgacg tccttgccat
      661 cgccgacggt caccttgagc gaaatggtgc cagggccctt gatgccgagg tcgcggatcg
      721 tgatcagccc atccggccga gttcgaacct gcaggcccgg ctcctcggcc accccgacga
      781 gcatcgcatc gtccccgtcc gggtccagcc agtccggcag gacgttgtag ctcacttcgt
      841 gacgttcggc gacctggacg ctggggaaac gccgctgttc cggggcctcg ttgaccgtgt
      901 ccgggtggac ctcgatggtc accgatgcgc gatcctcccc gccgcgcccg tcgctgacga
      961 tgtagggaat cgtctcgcgt cccgactcgg cgttcgtggt gatctgcagc gcctctccac
     1021 ctcgcaccgt ggagatgggt cccaggttgc cgggcttggg gacggcggcg gtcaagatgt
     1081 ccccgtcggg atcggagtcg ttcatcagga ccgggagctt cgtggcccgg cccgagcgca
     1141 cgccgaaggt gtcgtcgagg gcgcgcggag gatggttgtt cttgctgcgc ttgccggtcg
     1201 tgacttcgtc gatcgtctcg gcgctgtcct cgtcgccctt gtccttggac tcgatctgcg
     1261 cctcgatgtc cttccagttc gtgacggtcg tgaagtcggc gttcggcaag acgatgtcgc
     1321 catttgccgt gtcattgatg accacgacgt cccggttcac gcggaagacc agcgaggtga
     1381 tcttggcgag cctcctcacg gttcgagaca ggtcattcgt agtgcccgcg cagtcccgga
     1441 tgtaagcacc cgagccggac cacgcggcat acgtgcaccc accgacgacg accggcgcgg
     1501 ccggctttcc ccgctggaca ctggcgcgca ccggtgccga tccgtccaag gggatggcca
     1561 gcagcgagga gggtccggac aacgcgaccg agttggcggg cgggcccggt tgttgcagca
     1621 cgagcccggc ttcggggagg gccagggcgc ggtccttggt ccacattcgc ttggccgcag
     1681 cgtcggtggc gaccacctct tctccgacca gagtgaggac gatcccgtca gaaccggggt
     1741 aatcgggtag gcgaccgacg tccgcgacgc tccacccccc cgtggccggg gcgacagtcg
     1801 tgaggccgcc atcgcccgcc accgcgtgga cgacacccga gcgtgagacg acgacgcgca
     1861 cgccgcgcac gccttcgacc gtcggctcgc cctccgggga gaaggagccg atctgggcca
     1921 ggctcatcac ccacacccgt ccgcctgccg agtcgacgat ggcgacggtg tcgccaccgt
     1981 gggccagggc gaagccagac ggcacctcgc ccttgacccc gtacacgagg cgggccgtgt
     2041 caacgctctg ggcgaccccg gaggtggggt cggcaacgag gatgctcctc gcctcctgag
     2101 tcacatcgac ctcgtcgccg aaggcactca ggccaccgtc gagggtccgg gacgggtagt
     2161 tgaggtgccc gacgagccgg agggcggtat tggtgaccca cacgccgccg tcattgagcc
     2221 ggagctccgc ggtcttcacg cccggatgag cggcggcgaa ataccccaga acaagcgcca
     2281 gcagcgccag cccggccacg ctcgcacggg actggtcgac ctcgggccgc cgcacctcgc
     2341 cctcctcacc gcagcgggca ctgacgctcg ccattcacgg caaccttgct agctgtggcg
     2401 ggccaaggcg agtccccctt ttggacgaca tgacatccgg ggtcgtcacg tcggggattc
     2461 actcgcgagc cagcaaccct ctcaggtgcc atggacgtga gagcctggct ttccacgagg
     2521 cacctcgcga catggacttc cgggccttcg cgatgtcagc ccagtaggag gagaccgcgt
     2581 ctggggaagg gtcgtgagaa tcgaagacgg cccggtttgc ccggccggca agatcggcgt
     2641 gaccgatgac agccgcttgt tccaggcgtg tgtgcccggg ctcgacgtgg tggccgagat
     2701 cgcgggcgtg atcgatcagg tcgcgccagc cggcggccag ccgacggctg gcgtcacctc
     2761 gcgaccggcg acggctcctg cgtccggcgc gtaacccggc cagcaacagt ccggtgccgc
     2821 cgaccatggc gagcggtgga ccggcataac gcgccacggg agtgacccat ccggggaggg
     2881 actcggcagg aagacccggc aggccggggt tcgcttcggt aggccggccg gagggccgtg
     2941 gccgctgcag cggcgggaca ccctcgatga gcgagtcgac cgatccgggc gggcgccggc
     3001 cgaccggtgc cggaacgacg gcttgctcgg cgggcggggc gtcctcgtag tccgtctgct
     3061 tgggccgctt cgaccgttgt ggcatgaaga ggctgttggg cacggcgagc cattcccccg
     3121 aagcctcccg cacttccacc caggcatgga cgtcctgccc gcggacatga ccgtccccgg
     3181 gcacgatcgc ccccatcact acccgggccg caatgccggt ctgctgcgcg aggagggcga
     3241 acgcggcggc atactgttcg tcgctgccga ccggctgcgg gcccgcgagc aggcgcagga
     3301 gtcggccttg accgtgtccg ggccagtagt cctcctcccc ctggcgggtc ccgtcactgt
     3361 atgcgccggt ggtttcgagg tgcctgccga tggcggcgag gcgggcccac ggctgctcgg
     3421 cgtcgccggc ccactcggcg acccagggcg cgacgaacgc tcctcccgcc tggtcgatgg
     3481 cggccagcgg gtcgggctcg atcccggtct tggggacctc ccgcacgaca tatgtcgtct
     3541 gctcgatcac ggtgccggag gcgagacggg acggcaccag cagttggccg gtggccggat
     3601 tgaaccgaag gcttgtggcg gcgtccccgc cgtggggtcc ggcgaaatgt gcacgggtcg
     3661 gcgtccccag ccccgggacc caggatgcca actccggaat cgcggcatac gcgtctccga
     3721 tggtgacgga aatctgctgc ggctcaccgc ccggggtttc cggctcgggg aaggcgccac
     3781 ccacgcggtg gaagaccccg ccggtggcgg tcatcgtgcc ggcgctccac gtcgtgccgt
     3841 catacgaatc cagcaccgcg aaccgcaacc gggcatcccg cgcggcaccg gtcacggtga
     3901 ggagttcctg cccccacagc tggttcaggc ccgcgccgta gcggcggaac ccgaccaacg
     3961 gcgagacgag ctcgctgacg tcgaagggcg gctcgacgag cgtgcgggcg agcagccgtg
     4021 gacgggattc gactccgggc agatgcggga tcgcgatgcc ggtccccagc gccgataccc
     4081 cgagcagggc gaccgcgatg gcggcctggc ttattcgcgc tccacccgtc ccggccaccc
     4141 gacgcacgcg acggcgccgc acggcccccc agcacgccgt cgcggcagcg gtcaggacgg
     4201 cagcagacac cgcccacgcg cggttggtcg tgccgaggaa ggccatcaag cccaagacgg
     4261 tgatcggcac cgtgaccggc atccaggcgc ggggacggtg cgccagcaac cccccgatca
     4321 ccccggccgc caggcccagc gatacgggaa gcccacccag ggtcgtaccg acgtccaccg
     4381 gaggcagact gctgagcatg tccttccacc cggtgaccgg gaccggcgcc atacgccgga
     4441 agtcggtcgg cacgccgagg accgtgacgg gcgacagggc caggtaggcc gcgaccgctg
     4501 ccagcgtcgc cgcgagggct tgcgcggtgg cgcgccccgg tgctgacgcg cgtccagcaa
     4561 caatgattcc gattcgggca cagccgaatc cgaccagcgc cgcgacgacg atcatgagca
     4621 gatagcggaa cgagtcgtat gccggacgcc aggccatcgc cgcggccgca agcgatgccg
     4681 acagtccgat cgcgtcggcg aaggggtgtg cccgccacca cgcccgtgcg ggagcgtgcg
     4741 ccgccgagcg ggtggccgtc gtgagcgtgg ccgaactcat gctcgcctcc tccccgccaa
     4801 ggcggccggc agatcctcga gcgatccgaa ggtcaccagc gtcatgccgc tggcgtgctg
     4861 cagtcgcacg tcggcgccgg gttcgaaccg caacacgtgg acgttgaccc ctggcgcgag
     4921 ggacgtccgg gcccgggcca cgcgcgccag gtccgcgtgc tcgccgatga cgagcgcgac
     4981 ggaactcaac gtgggagcga actgcaccag tctgcccgtg gcggcgtcga gctgctcggg
     5041 tccgaggcgg gcgcgggcga agaggtccag cgccgcgttc ccgcgagggc cccgcaggac
     5101 gaaagaacct gctgcgacac tgagctcgat cttgtcgtgg gccgcccgct gggcgatcga
     5161 ggcagccgcg gacaagacgg tctcgaagtg atcgggatcg gcgtatgacg tggcggcgca
     5221 gtctgccacc actccgaccg aggtgcggcg agtgtcgagg aattgccgga ccatcagatc
     5281 actctggcct gcggcgctgc tgcgtttggc ggtcgagagc caatggatgt gccgccgatc
     5341 atcgccgggc acgtactcac gcaggccgtg gaaggcgaga tcgctggacg agacgtcctc
     5401 tgtcgtgcgg ccctctaggt cacgcaagat cccgctggtg aaaccgtcca cgtagacggt
     5461 gtggggatgc acgatgagtt cgaccggatc ggtccggcgt actgcgcgcc gcacgaggcc
     5521 gtacgggtcg ccgcgcaggg tggtcgccgg cccgatcgga tagacgccac gcctgccggt
     5581 cgggaggatc accacgtcgt cgaagctctc ggtgggccgg atcgccggca actggacggc
     5641 aacggctgac tccccgatgc ccagttccag cggcatcgca ggcaccggaa ggcggccgtc
     5701 gttgcggact cggacttcgg cggctgccgc ttcgccgacc cggacccggg tgggctgcac
     5761 ccgcacccgg acgtcgagct gggtgcgtcc gatcgtgagc aggcaactca gcgcgaagag
     5821 cagagtcagc atccccgcga gatagctcag ttcgatccag cctccaaccc agccgacgac
     5881 ccacgccacc acggccgttg ccaacacggc ccagcccagc ggcgagacgg cgtccagcac
     5941 cggatcgacg tgctcaccca ggctagggac ggtatgccgg cgctgggaca ctgtcgatca
     6001 gctcgtcgat gacatccccg acccgcacgc cggagaactg cgcctcgtcg ccgaggagta
     6061 tgcggtgggc aagcaccggc tgcgccaagc ccttcacgtc gtcgggcgtg acgtagggcc
     6121 ggccttggga aagggcccag gtctttgcgg agcgcacgaa ggccagaccg ccgcgcacgc
     6181 tcagtccgag ggagacgtgg ctgtgccggc gcgactcctc gccgatgagg gcgatgaagc
     6241 tgaggatgga ggggtcgacg tggaccaggc cggcctgcgc ggacatggcg cggaccgaat
     6301 cggcgcgcag gataggttcg atggccccgc tgcggtcccg gttctcggcg tccgcgagca
     6361 actccacggt cgtgtcgtgg tcggggtaac cgagcgaggt cttcattaag aagcggtcga
     6421 gttgtgcctc gggtagccgg taggtgccgg cctgttcgat cgggttctgg gtcgcgatca
     6481 ccatgaaggg cgagccgaca ccatgcggct ggccgtcgac ggtcacgcgg ccctcctcca
     6541 tgacctcgag gagggcggat tgcgtcttcg gcgaggcacg gttgatctcg tcggcgagca
     6601 cgatcgaggc gaagaccggt ccgggccgga atgcaaatcg ctgggttgat tggtcataga
     6661 cgctgacacc ggtgatgtcc gtcgggagca agtcgggggt gaactggatg cggctgtggg
     6721 tgccctcgat cgtgtttgcc agggccttgg ccaactgggt cttgccggtg cccgggttgt
     6781 cctcgacgag caggtgtccg cccgcgatca ggcacgtgag cgccagccgc gtgacatatg
     6841 tcttgccgcg caccgccttc tcgatattgg cgaccatctg gtcgaaggcc cccgcgaaat
     6901 gggtggcgtc ctggtcggtg agcgtcatga acctttcttt ctgtgcggtc cggttccggg
     6961 tgtcgaggag cggtggaggg tcggtgctgg caggggtagc gacggcgggt gggtcggtgc
     7021 tggtaggggt ggcgacggcg ggtgggtcgg tgc
//
LOCUS       DAOPJG010000173         7033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_11387_length_7033_cov_8.193322, whole genome shotgun sequence.
ACCESSION   DAOPJG010000173 DAOPJG010000000
VERSION     DAOPJG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11387_length_7033_cov_8.193322"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /gene="glmS"
                     /locus_tag="PKU75_13525"
     CDS             <1..1826
                     /gene="glmS"
                     /locus_tag="PKU75_13525"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPF81944.1"
                     /translation="DEKALTVVMEGLARLEYRGYDSAGVALVTEDGIEAEKRAGKLEN
                     LRSALVAHPLDGSRTAIGHTRWATHGGPTDGNAHPHRGGQDDKLALIHNGIIENFHSL
                     KKGLLAAGAQFQSETDTEVAAKLVGAEFDKTGDLTTAMLNAVQQLEGAFTLLAIHADQ
                     PGVVVGARRNSPLVVGLGDGENFLGSDVAAFIGYTRQALELGQDQIVTITPDGYSVMG
                     FDGRPAEGTHYEVTWDAAAAERGGYATFMEKEINDQPHAVADTLLGRTDESGALVLDE
                     VRISEEQLRAVDKITVVACGTAAYAGMVAKYAIEHWARIPVEVALAHEFRYADPVVDE
                     RTLVVSISQSGETMDTLMAVKHARELGAVTVSICNTHGSTIPRESDAVLYTHAGPEIA
                     VASTKAFLAQITAAYILGLYLAQLRGGSYAEDAQAVMRDLADMPAKIQDLLGRMDRVK
                     EMAEFMADTRSVLFLGRNVGYPVAMEGALKLKELAYIHAEGFAAGELKHGPIALIDAG
                     QPVFVIVPSPDTPHEVHKKVVSNIQEIRARGARTLVIAHDGDEDVEPFASEVIRIPAC
                     PPMLAPLLAIVPLQVFALQLSTAKGLDVDQPRNLAKSVTVE"
     gene            1863..2222
                     /locus_tag="PKU75_13530"
     CDS             1863..2222
                     /locus_tag="PKU75_13530"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPF81945.1"
                     /translation="MIVGVGIDVVDIARFGETLTRTPRLKERLFTSAERELPLDSLAA
                     RFAAKEALAKALGAPVGLDWQDAEVHRGQDRRPHFEVRGTVAARLAELGVATIHLSIS
                     HDAGVASAVVVCEGGAS"
     gene            2219..3697
                     /locus_tag="PKU75_13535"
     CDS             2219..3697
                     /locus_tag="PKU75_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPF81946.1"
                     /translation="MIDAYAVKDVYAAEEVLLAALPEGELMARAVAGLAEVARVRLEQ
                     EGGTRVVALVGPGNNGADAMYAAARLAGRGFASAAIYLADRVHQGALSSAIAAGVVLK
                     PITPDSPADAVDRFLDEADLVIDGIVGIGGRPGLPEFAQPWVAAIPDDAYVLAVDLPS
                     GQDPMGDAADACGVWADETVTFSAAKPVHLSPATEPACGMLTVIDIGLDLAADIGPEQ
                     AAVRRVERADAARLWPVPGPRDDKYSRGVVGVIAGGENYTGAAVLAVTAAANAGAGMV
                     RYVGTPTPDALVRAAVPEAVHGVGQVQAWVVGPGLDVKARHAGAKAQLDAAREALAGD
                     APVVVDAGGLDLISGHRAAPTLLTPHAGECARLLSRLRGTQVTRSDVAHHPLPFAREL
                     ADRTGATVLLKGAWTIVTRADGPAYSQNDAPSWLATAGAGDVLAGLLGTLLAAGLDPL
                     DAGALGALVHGLAADRANPGGPVRARAVADALPAVIADLLRS"
     gene            complement(3714..4580)
                     /locus_tag="PKU75_13540"
     CDS             complement(3714..4580)
                     /locus_tag="PKU75_13540"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HPF81947.1"
                     /translation="MPPLDPSTLVAELLGRPPVAATRLGGGDIADAWLLTLEGGERAV
                     LKTMSGADADFFAAEARGLEWLHTPQGPPVPRVLAAGPAGIVMEYAAPGAPTPDAAAD
                     FGRRLARMHAASPGGFGAPWPGFIGSIPMDNASPDGESWADFFARCRVAPYVRAARDR
                     GSLSATDAHVIESLLPRLADLVPAEPPARLHGDLWSGNVHWGKGSRVWLIDPAAHGGH
                     RESDLAMLALFGAPHLDAIVTAYQEEAPLATGWRDRIPIHQLFPLLVHAVLFGGGYGP
                     RAAAGVAPFAAR"
     gene            4671..5867
                     /gene="alr"
                     /locus_tag="PKU75_13545"
     CDS             4671..5867
                     /gene="alr"
                     /locus_tag="PKU75_13545"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPF81948.1"
                     /translation="MDTTAAPTTPPAHRPTATVPAGVSGCVEIDLSAISDNVRELRRR
                     AGDAEVMAVVKADGYGHGALPAARAALAGGATWLGVAQLAEALTLRGAGVEAPMLTWL
                     YVPGAEVEAALRADIDVTISADWQLRQVLDAARSIGSPARVHLKVDTGLARNGAFGAA
                     WSDLVRGAAAAAAEQAIEVVGVWSHFAYADAPDHPTVRQQQERFVEAVAELEREGVHP
                     QLRHLANSAATLTNPSAHYDLVRPGLAVYGLSPTPDLGSSADYGLREAMRLVARLSLV
                     KDIPQGQGVSYGHSYLTDRDTRLGLVPLGYSDGIPRNASNAGPALLDGRRLTIAGRVC
                     MDQFLLDLGPDATAEPGDEVVLFGAGALGEPTAEDWAAATGTISYEIITRVGARVPRY
                     YVEGQG"
     gene            5867..>7033
                     /locus_tag="PKU75_13550"
     CDS             5867..>7033
                     /locus_tag="PKU75_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF81949.1"
                     /translation="MADRRLTAATIGAAAAAGAVTAAGAMVRRATVRRAGLEGIEALE
                     LGASNPFEWYADRELVVTADDGAQLHVEIDEPQGFEGGPTVVFSHGYCLALGSWIHQR
                     RAVVQAGFRCVAWDQRGHGRSGTGAEGHSTIDQLGSDLERVIAAAAPDGPLVLVGHSM
                     GGMTVMALADRNPALVRDRVDAVGFVSTSAGGDGMISLGFGRMLGATLLRFGPGVLAN
                     LAARQRLWRAARSVGRDVESYLVERFSFASPVSAETVRFAADMLMGTDLKVVADFLPA
                     FEDHDKRDALPVFGRVDALVLNGEGDVLTPPDHSQAIVDVLPNAEHVVITDAGHLVML
                     EHPALVDAQLLALIDRAHVEEAPAPARGRTQITDIARRHAVQSLRQKRTGQKRTG"
BASE COUNT         1017 a         2610 c         2429 g          977 t
ORIGIN      
        1 tcgacgagaa ggccctcact gtcgtcatgg aagggctggc ccggctcgag taccgcggct
       61 acgactcggc gggagtcgcg ctggtcaccg aggacggcat cgaggccgag aagcgggccg
      121 gcaaactcga gaacctccgc agcgccctgg ttgcccaccc cctcgacggt tcccgcaccg
      181 ccatcggtca cacccggtgg gccacccacg gtggcccgac cgacggcaac gcccacccgc
      241 accgcggcgg ccaggacgac aagctcgccc tgatccacaa cggcatcatc gagaacttcc
      301 actccctcaa gaagggtctg ctcgccgccg gtgcccagtt ccagagcgag accgacaccg
      361 aggtggcggc caagctcgtc ggcgccgagt tcgacaagac cggcgacctg acgacagcca
      421 tgctcaacgc cgtccagcag ctcgagggcg ccttcaccct gctggcgatc cacgccgacc
      481 agcccggcgt cgtggtcggt gcccgccgca acagcccgct ggtcgtcggt ctcggcgacg
      541 gcgagaactt cctcggctcc gatgtcgccg ccttcatcgg ctacacccgc caggcgctcg
      601 aactcggcca ggaccagatc gtcaccatca cccccgacgg ctacagcgtg atgggcttcg
      661 acggccggcc cgctgaaggc acgcactacg aggtcacctg ggatgccgcg gccgccgaga
      721 ggggcggcta tgcgaccttc atggagaagg agatcaacga ccagccgcac gccgtcgccg
      781 acaccctcct gggccgcacg gacgagtccg gcgccctcgt cctcgacgag gtgcgcatca
      841 gcgaggagca gttgcgggcg gtcgacaaga tcaccgtcgt cgcctgcggc accgccgcgt
      901 atgccggcat ggtcgccaag tacgccatcg agcactgggc gcgcatcccc gtggaagtcg
      961 ctctggcgca tgagttccgg tatgccgacc cggtcgtcga cgagcgcact ctcgtcgtct
     1021 cgatcagcca gtccggcgag acgatggaca ccctcatggc cgtcaagcac gcccgtgagc
     1081 tcggcgcggt gacggtctcg atctgcaaca cccacgggtc gacgatcccg cgcgagtccg
     1141 acgccgtcct ctatacccac gccgggcccg agatcgccgt cgcgtcgacg aaggccttcc
     1201 tcgcccagat caccgccgcc tatatcctcg ggctctatct ggcccagctg cgcggggggt
     1261 cgtatgccga ggacgcccag gccgtcatgc gcgacctcgc cgacatgccc gcgaagatcc
     1321 aggacctgct cgggcggatg gaccgggtca aggagatggc ggagttcatg gccgacaccc
     1381 gctcggtgct cttcctgggc cgcaatgtcg gctaccccgt ggcgatggag ggcgcgctca
     1441 aactcaagga gcttgcctat atccatgccg aaggcttcgc cgccggcgaa ctgaaacacg
     1501 gcccgatcgc cctcatcgac gccggtcaac cggtgttcgt catcgtgccc agccccgata
     1561 ccccccacga agtgcacaag aaggtcgtct ccaatatcca ggagatccgc gcccggggcg
     1621 cccgcaccct cgtcatcgcc cacgacggcg acgaggacgt cgagcccttc gccagcgaag
     1681 tcatccgcat cccggcgtgc ccgccgatgc tggccccgct gctggccatc gtcccgctgc
     1741 aggtcttcgc attgcagctg tcgacggcca agggcctcga cgtcgaccag ccgcgcaacc
     1801 tcgccaagtc cgtcactgtg gagtagtgcc gccgggtgcg ggtcgcgtgg aatgatcggc
     1861 acatgatcgt cggagtcgga atcgatgtcg tcgatatcgc gcgcttcggc gaaaccctca
     1921 cgcgcacacc gcgtttgaaa gagcgactct tcacttcggc cgagcgggaa ctgccgctcg
     1981 actcccttgc cgcgcgcttc gcggccaagg aggctctcgc gaaggcgctc ggcgctcccg
     2041 tcgggctcga ctggcaggac gccgaggtgc atcgcggcca ggaccgccgg ccgcacttcg
     2101 aggtacgcgg caccgtggcc gcccgcctcg ccgaactcgg ggtggccacc atccatctgt
     2161 ccatctccca cgacgcgggc gtcgcctccg ccgtggtcgt ctgcgaggga ggcgcgtcgt
     2221 gatcgatgcc tacgccgtca aggatgtgta tgccgcggag gaggtcctgc tcgccgcctt
     2281 gccggagggt gagttgatgg cccgcgccgt ggccgggctc gccgaggtgg cgcgggtgcg
     2341 gctggagcag gaaggcggca cccgggtcgt cgccctcgtc ggtccgggca acaacggcgc
     2401 cgatgccatg tatgccgcgg cacgccttgc cggccgcgga ttcgcctccg ccgcaatcta
     2461 tctcgccgac cgcgtgcacc aaggagcctt gagttcggcg atcgccgccg gggtcgtcct
     2521 caaacccatc accccggaca gcccggccga cgccgtcgat cggttcctcg acgaggccga
     2581 cctggtcatc gacgggatcg tcggcatcgg gggccggccc ggcctgccgg agttcgccca
     2641 gccctgggtc gccgccattc ccgacgatgc ctacgtgctg gccgtggacc tgccatcggg
     2701 ccaggacccc atgggagacg cagcggacgc ctgcggagtc tgggccgacg agacggtgac
     2761 cttctccgcg gccaagccgg tgcacctgtc gccggcgacg gaacccgcgt gcgggatgct
     2821 gaccgtcatc gacatcgggt tggacctcgc cgccgacatc ggcccggagc aggcggccgt
     2881 ccgccgcgtc gaacgcgccg atgccgcccg cctgtggccg gtccccggcc ctcgcgacga
     2941 caagtactcc cgcggcgtcg tgggcgtcat tgcggggggc gaaaactaca cgggcgcagc
     3001 ggttctcgcc gtcactgcgg ccgccaacgc cggcgcgggg atggtgcgct atgtcggcac
     3061 cccgacccct gacgcgctcg tgcgggcggc cgttcccgag gccgtccacg gtgtcggtca
     3121 ggtgcaggcc tgggtggtcg ggcccggtct cgatgtcaag gcccggcacg ccggagccaa
     3181 ggcgcaactc gatgccgccc gcgaggccct ggccggggac gccccggtgg tcgtcgatgc
     3241 cgggggcctg gatctcatct ccggccaccg cgccgcaccg acgctcctca ccccgcatgc
     3301 cggggagtgc gcccggctgc tctcccggct gcgcggcaca caggtcaccc gctccgacgt
     3361 cgcccaccac ccgctcccgt tcgcccgcga actcgccgat cgcaccggcg ccacggtgct
     3421 gctcaagggc gcctggacga tcgtcacccg ggccgacgga ccggcataca gccagaacga
     3481 cgcaccctcg tggctcgcga ccgccggcgc gggcgacgtc ctcgcagggc tgctcggcac
     3541 tctcctcgcc gccgggctcg acccgctcga cgccggcgcc ctcggggcac tggtgcacgg
     3601 cctggccgcc gatcgcgcga accccggcgg gccggtgcgc gcccgcgccg tcgccgacgc
     3661 cctcccggcc gtgatcgcgg acctgcttcg cagctgaggg tgccggtcgc gggtcagcgc
     3721 gcggcgaagg gtgccacgcc ggccgcggcc cgggggccgt agccgccgcc gaagagcacc
     3781 gcgtgcacca gcagcgggaa gagctgatgg atgggtatgc ggtcgcgcca gccggtcgcc
     3841 agcggcgcct cctcctggta tgccgtgacg atcgcgtcga ggtgcggcgc accgaagagg
     3901 gcgagcatcg ccaggtcgct ctcgcggtgc ccgccgtggg cggcggggtc gatgagccac
     3961 acccgagagc ccttgcccca gtgcacattt ccgctccaca ggtccccgtg caggcgggcc
     4021 ggaggttcgg cgggcaccag gtccgcgagc cggggcagga gcgactcgat gacatgggca
     4081 tcggtggcgg acaaggaccc gcggtcccgg gcggcccgaa catagggcgc cacccggcac
     4141 cgcgcgaaga agtccgccca ggactcaccg tccgggcttg cgttgtccat cggaatgctg
     4201 ccgatgaacc ccggccacgg tgcaccgaaa ccgccgggcg aggcggcatg catccgggcg
     4261 agccgccggc cgaagtccgc cgcggcatcg ggcgtggggg caccgggcgc ggcatactcc
     4321 atgacgatcc ccgccggtcc ggcggccagc actcgcggga cgggcggccc ctgcggcgta
     4381 tgcaaccact ccagaccccg cgcctccgcg gcgaagaagt ccgcatccgc tccgctcatc
     4441 gtcttcagca cggcgcgctc gccaccctcg agggtgagca gccacgcatc cgcgatgtcg
     4501 ccgccgccga gacgggtcgc cgcgaccggg gggcggccga ggagttcggc gaccagcgtg
     4561 ctggggtcga gcggcggcat acgacccact atgccccgat ccactcgtag gcccgccgac
     4621 ggcgcgcgtg agaggtggcg acgccccgtc gaactagact cggagaccac atggacacca
     4681 cagccgcgcc gaccaccccg cccgcccacc gacccaccgc cacggtgccg gcgggggttt
     4741 cgggctgtgt cgagatcgac ctgagcgcca tctccgacaa tgtgcgcgaa ctgcgccgtc
     4801 gcgcgggaga cgccgaggtc atggccgtgg tcaaggcgga cggctacggg cacggcgccc
     4861 tgccggccgc gcgcgccgcg ctcgccgggg gcgccacctg gctcggagtc gcccaactcg
     4921 ccgaggccct caccctgcgc ggcgcgggcg tcgaggcccc gatgctcacc tggctctacg
     4981 tgccgggcgc ggaggtcgag gcggcactgc gagccgatat cgacgtgacg atctccgccg
     5041 actggcagct gcggcaggtg ctcgacgcgg cccgcagcat cggctcaccc gcacgcgtcc
     5101 acctcaaggt cgacacgggg ctggcccgca acggcgcctt cggcgccgcg tggagcgatc
     5161 tcgtgcgcgg cgcggccgcg gcggcggccg agcaggcgat cgaggtcgtg ggggtctggt
     5221 cgcacttcgc gtatgccgat gcgcccgacc acccgacggt gcgccagcag caggaacgct
     5281 tcgtcgaggc ggtcgcggaa ctggagcgcg agggcgttca cccgcagctg cggcatctgg
     5341 cgaattccgc tgcgacactg acgaacccat cggcgcacta cgacctcgtc aggccgggcc
     5401 tggcggtcta cgggctctcg ccgacgccgg acctgggcag cagcgccgac tacggcttgc
     5461 gcgaggcgat gcgcctggtt gcccggctca gcctggtcaa ggacatcccg cagggtcagg
     5521 gcgtcagcta cggccactcc tacctcaccg accgggacac ccggctcggg ctggtcccgc
     5581 tcggctactc cgacggcatc ccgcgcaatg cctcgaacgc cggaccggcg ctgctcgacg
     5641 gccggcggct gaccatcgcc ggtcgcgtct gcatggatca gttcctcctc gatctcggac
     5701 ccgacgccac ggccgagccc ggcgacgagg tggtgctctt cggggccgga gcgctcgggg
     5761 agccgacggc tgaggactgg gcggcggcga ccgggaccat cagctacgag atcatcacgc
     5821 gcgtcggggc gcgggtgccg cgttactacg tcgaaggtca gggctgatgg ccgatcgccg
     5881 gctgaccgcc gcgacgatcg gtgctgccgc ggccgcgggt gccgtgaccg ccgcgggcgc
     5941 gatggtgcgc cgggccacgg tgcgtcgggc cgggctagaa ggcatcgagg ccctcgaact
     6001 cggcgcgagc aatcccttcg agtggtatgc cgatcgcgaa ctcgtcgtca cggccgacga
     6061 cggtgcgcag ttgcacgtgg agatcgacga accacagggc ttcgagggcg gtcccaccgt
     6121 cgtgttctcg catggctatt gcctggctct cggctcgtgg atccatcagc gacgggccgt
     6181 cgtgcaggcc ggattccgtt gtgtcgcttg ggatcagcgg ggccacggcc ggtccggcac
     6241 gggggcggag ggccattcga ccatcgacca gctgggcagc gatctcgaac gggtgatcgc
     6301 ggcggcggcg cccgacggcc ccctcgtcct cgtcggccac tccatgggcg ggatgaccgt
     6361 gatggcgctg gccgaccgca accccgccct ggtccgggat cgcgtcgacg ccgtgggctt
     6421 cgtctcgacg agcgcgggtg gcgacggcat gatctcgctc ggcttcggcc ggatgctcgg
     6481 cgcgaccctg ttgcgtttcg gcccgggcgt cctggccaat ctcgctgccc ggcaacggct
     6541 gtggcgtgcg gcccgctccg tcggccggga cgtcgagtcc tatctcgtcg aacgcttctc
     6601 cttcgcctcc ccggtctcgg ccgagacggt gcgctttgcc gcggacatgc tgatgggcac
     6661 cgacctgaag gtcgtggccg acttcttgcc ggcgttcgag gatcacgaca aacgcgacgc
     6721 gctgcccgtg ttcggccggg tcgatgccct cgtcctcaac ggggagggcg acgtgctcac
     6781 gcccccggat cacagccagg cgatcgtcga tgtcctgccc aacgccgagc acgtcgtcat
     6841 caccgacgcg ggccacctgg tgatgctcga gcatccggcg ctcgtcgacg cccaacttct
     6901 ggccctcatc gatcgagccc acgtcgagga ggcgccggcc cccgcgcgtg gtcgcacgca
     6961 gatcaccgac attgcccggc gccacgccgt gcaatcgttg cggcagaagc gaaccggaca
     7021 gaagcgaacc gga
//
LOCUS       DAOPJG010000174         6999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_11487_length_6999_cov_6.369816, whole genome shotgun sequence.
ACCESSION   DAOPJG010000174 DAOPJG010000000
VERSION     DAOPJG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11487_length_6999_cov_6.369816"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKU75_13555"
     CDS             complement(<1..714)
                     /locus_tag="PKU75_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPF81950.1"
                     /translation="MIIDAHGHYTTAPPQLEEWRNRQIAAIGDPANAPRPEDLVISDD
                     KIRESIETNQLRLMDERGSDLTVFSPRASFMAHHIGDYAVSETWSRICNDLCHRVATL
                     FPDRFAMGAMLPQSPGVDPATVIPELRRAVEELGAVVVNLNPDPSGGHWDSPPITDTQ
                     WYPIYEVLSELDVPAMVHVSTSCNPAFHTTGAHYLNADTTAFMQLIQGDLFADFPDLR
                     LIIPHGGGAAPYHWGRFRGL"
     gene            complement(763..1374)
                     /locus_tag="PKU75_13560"
     CDS             complement(763..1374)
                     /locus_tag="PKU75_13560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPF81951.1"
                     /translation="MQLRHATSADRDQIASVTRSAFEEGEDGPVARVLEMLERTEAVR
                     ASLVATEDDRIVGHVQLNRSWIDARERLVDVLVLSPLSVAPDRQGQGIGTALVAAALA
                     EAERLGDPAVFLEGGWDYYGERGFRRASEFGFSRPSPRIPDRAFQVALLPSWQAWMVG
                     SLVYCEAFWATDTVGLRDPLLAQIEARSAGSPTEEPPQTPASA"
     gene            complement(1416..2177)
                     /locus_tag="PKU75_13565"
     CDS             complement(1416..2177)
                     /locus_tag="PKU75_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPF81952.1"
                     /translation="MPIPLDDLGAPVPVPERVCRVVSLVPSLTEALAASCPHLLVGAT
                     DWCTHPAGLDVARVRGTKNPDLDAIAALAPDLVVANMEENRELDVRRLRERGVPVWVT
                     RIETVPDAFASMRRLFTEVIGLHQDPPWLSEAEARWPVTVPAAHTRVVVPIWRDPWMI
                     VGTPTFTADLLARAGYAVCSPSPGRYPHADLAEMDDPGLADLALLPDEPYVFSQDDGP
                     EAFSRIPTRLISGRLITWYGPALLEAADELLGNAG"
     gene            2257..2964
                     /locus_tag="PKU75_13570"
     CDS             2257..2964
                     /locus_tag="PKU75_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPF81953.1"
                     /translation="MAVDANHPVLGLSSMATKAVLTALTAPGVLDAPSTRFESGGGVA
                     IAQRIREGEVADIAVLASGAIDKLVAEGLLDAGTIRPVFCSEGVIAVPEGAVAPNISS
                     VATVQEVFAAAEGIGYSTGPSGDALLALIDDWGLRDSIGARLVQAPPGVPVASLIAEH
                     RVAIGVQQRSELADRAGVRIVGGMPPGAEMSTTFSGAVLTASPRPEDADRVLAALAGP
                     TAYETIRRHHLQPATDL"
     gene            complement(2965..3483)
                     /locus_tag="PKU75_13575"
     CDS             complement(2965..3483)
                     /locus_tag="PKU75_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPF81954.1"
                     /translation="MSCSSTGSPTPTPVVNATLIGGRLPMPLSSSGVVLLAHADESVL
                     EDVVRAGWPQPTPHALPDARALRDLLAMVRRRGYAALDGHIHPDSRGVAVPVLGPLGS
                     VYAALSVVVPNDDAPVQRVVEVLKVAAAGIGRELRAADLPDGVVTGDATLRPLISTSR
                     ATLDYFASIDGG"
     gene            complement(3656..3766)
                     /locus_tag="PKU75_13580"
     CDS             complement(3656..3766)
                     /locus_tag="PKU75_13580"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPF81955.1"
                     /translation="MDALDARRPFLTLSEIAQVAGLPLSSTHRLVAELTH"
     gene            3781..4284
                     /locus_tag="PKU75_13585"
     CDS             3781..4284
                     /locus_tag="PKU75_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HPF81956.1"
                     /translation="MAYVDDLAAYPDKVTVNCSDTDGRAALDRWRPDDPRVRVYACGT
                     ARLLDDVSAWGAVTGGFPPKIERFVAATADAGRPRAAFEVRAARSDVTTTVAPHETIV
                     AAPRRAGVEVITSCAQGVCGTCETEVLEGEPDHRDSLLDDSEREIARCLFPCVSRSRS
                     ERLVLDL"
     gene            4312..4875
                     /locus_tag="PKU75_13590"
     CDS             4312..4875
                     /locus_tag="PKU75_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81957.1"
                     /translation="MTTAIAFCESDIDPFDEEFLADPLPRLAQLRDLGPVVYLSRYDA
                     HAVARYADVHRALTDWQSLQSGAGVGLSNFNHEEPWRPPSLLLEADPPRHDAPRHVLQ
                     QILSPRRLRALSDRWRSNAEQVVDDVLGRGDEFDAVTEIADVFPSGSSPMPSVSHAQA
                     PLMVRSLLTAGVDTTVTGIAAEHLARV"
     gene            complement(4953..6170)
                     /locus_tag="PKU75_13595"
     CDS             complement(4953..6170)
                     /locus_tag="PKU75_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HPF81958.1"
                     /translation="MTDASVPTPVDSTETAEVAIVGAGPAGLMLAHWLDRAGIDVVNI
                     DTRTREEIETTHRAGILEADAARDLVETGVSDRILRDGYEHEGTYLRFGGQSHHINFK
                     ELVGQSVWLYPQTDVFIDLADARARDGGRVHFGVTDPAVAGETTERPTMSFTGADGNR
                     TEVQARYVVGADGSRSMCRYLIPEAERRQFFREYPFAWFGLMTEAPMSAPELVYAHSE
                     HGFALISQRTEALQRMYFQCDPEEDINAWSDERIWETLQARVAGGDGFSLKEGPITEK
                     VVLRFRSFVQQPMRWGSLLLAGDAAHTVPPTGARGLNLALHDVKILADVLTRALGSDG
                     EAALADYEPRALGRVWRAQNFSYWMTQMLHAAPGGLEFDLMRQLGELDNVVNTRAGRT
                     YLAEQYTGWPSKS"
     gene            complement(6283..>6999)
                     /locus_tag="PKU75_13600"
     CDS             complement(6283..>6999)
                     /locus_tag="PKU75_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPF81959.1"
                     /translation="GTFTRERPSQSLSQIAKRTGLALSTTHRIVSELAAWGALERTAD
                     GDWHIGLRLWEVGSVCPRGQILREAALPFMQDLYEATHENIQLAVREGTEMVFVERIA
                     GHRSVELRTMVGLRFPLLGTAMGMVLLAYAPSEIRDAVLAGPMDRHTPHTVTDARTLR
                     SMLAQVRRATFAVNDRQFQDSTVGVAAPVRIGERGDVEAALGIIVAGATSAAVRHLRH
                     PVVSAARGVSRTLGERAGTP"
BASE COUNT         1058 a         2399 c         2506 g         1036 t
ORIGIN      
        1 caggccgcgg aagcggcccc agtggtaggg cgcggcgccg ccgccatgcg ggatgatcag
       61 cctcagatcg gggaagtcgg cgaagaggtc gccctggatc agttgcatga aggccgtggt
      121 gtcggcgttg agatagtgcg cgccggtggt atggaaggcc ggattgcacg acgtcgagac
      181 gtggaccatc gcggggacat cgagctcgct gagcacctcg tagatcgggt accactgcgt
      241 gtcggtgatg ggcgggctgt cccagtggcc gccggaggga tcggggttga ggttgacgac
      301 gacggcgccg agctcctcga cggcgcggcg cagctcgggg atcaccgtgg cggggtcgac
      361 gccgggcgac tgcggcagca tcgcgcccat ggcgaagcgg tcggggaaga gcgtcgcgac
      421 gcggtggcac aggtcgttgc agatccggct ccacgtctcg gagacggcgt agtcgccgat
      481 gtggtgggcc atgaagctgg cgcgtgggct gaagaccgtc aggtcgctgc cgcgttcgtc
      541 catcagccgc agctggttgg tctcgatgct ctcgcggatc ttgtcgtcgg agatgacgag
      601 atcctccggc cggggagcgt tggccggatc accgatcgcg gcgatctggc gattgcgcca
      661 ctcctccagc tgcggcgggg cggtggtgta gtggccgtgg gcgtcgatga tcaccggccc
      721 agggtggcag ccaggcctgt tcgccgtcag gaggtctttc cgtcaggcgg aagctggcgt
      781 ctggggcggc tcctcggtgg gcgagccagc cgagcgagcc tcgatctgcg ccaacagcgg
      841 gtcgcgcaat ccgaccgtgt cggtcgccca gaaggcctcg caatagacga gagacccgac
      901 catccacgcc tgccacgagg gcagcagggc gacctggaag gcgcggtcgg gaatccgcgg
      961 cgacggccgc gagaatccga actcggacgc gcgccgaaac ccgcgctcgc cgtagtagtc
     1021 ccagccacct tcgaggaaga cggccggatc tcccaaccgc tccgcttcgg caagggcggc
     1081 cgcgaccagg gcggtgccga tgccctgtcc ctgccggtcg ggggcaacgg acagcgggct
     1141 cagcaccagc acatcgacca ggcgctcgcg ggcgtcgatc cacgaccgat tgagctggac
     1201 gtgaccgacg atgcgatcgt cctcggtggc gacgagcgag gcacggacgg cttcagtgcg
     1261 ttcgagcatc tccaacaccc gcgccaccgg gccgtcctcg ccttcctcga aggccgagcg
     1321 ggtcacggac gcgatctgat cgcgatctgc cgacgtggcg tgacgcagct gcataccagt
     1381 gaccctagcg agcgggttcg cctgtgcgcc aagggttatc cggcgttgcc gaggagttcg
     1441 tcggcagcct cgaggagggc cgggccgtac cacgtgatga gccgcccgga gatcagccgg
     1501 gtcggaatgc gggagaacgc ctcggggccg tcgtcttggc tgaagacata gggctcgtcg
     1561 ggcagcagcg cgagatcggc cagcccgggg tcgtccatct cggcgaggtc cgcgtgcgga
     1621 tagcggccgg gggagggcga gcacacggca tacccggctc gggccaacag gtcggcggtg
     1681 aaggtcggcg tgccgacgat catccacggg tccctccaga tcgggacgac gacccgggta
     1741 tgcgctgccg gcaccgtcac cggccagcgc gcctcagcct cggagagcca tggcggatct
     1801 tggtgcagcc caatgacttc ggtgaacagt cggcgcatcg aagcgaaggc gtccggcacc
     1861 gtctcgatgc gggtgaccca caccggcacc ccgcgctcgc gcagcctccg gacatcgagc
     1921 tcgcggttct cctccatatt ggcgaccacg aggtccggcg cgagcgcggc gatggcgtcg
     1981 aggtcggggt tcttcgtgcc gcgcacgcga gccacatcca ggccggcggg gtgggtgcac
     2041 cagtccgtcg caccgaccag caggtgggga cacgaggccg ccagggcctc ggtgagcgag
     2101 ggcacgaggg agaccacgcg gcatacccgc tccggcaccg gcacgggcgc gccgaggtca
     2161 tcgaggggaa tcggcacgtc ctcacggtat gcggcgtcgc ttccgctccg cggaagggca
     2221 gggcccggcg tggcccacgg ctcatacggt gtgcccatgg cagtggatgc gaatcacccg
     2281 gtcctcgggt tgtcctcgat ggcgacgaag gccgtgctca cggcgctgac cgctccgggc
     2341 gtgctcgacg cgccgtcgac acggttcgag tccggcggtg gcgtcgcgat cgcccagcgg
     2401 attcgtgagg gcgaggtcgc cgatatcgcc gtgctggcgt ccggagccat cgacaaactc
     2461 gttgccgagg ggctgctcga cgccgggacg attcgcccgg tcttctgctc cgaaggcgtg
     2521 atcgcggtgc cggaaggcgc tgtggcacca aacatttcga gcgtggcgac cgtccaggag
     2581 gtcttcgccg cggccgaggg gatcggctac tccaccggcc ccagcggcga cgcgctgctc
     2641 gccctcatcg acgactgggg gctgcgcgac agcatcggcg cgcggctggt gcaggcgcct
     2701 cccggagtgc ccgtcgcaag cctcatcgcc gaacatcggg tggcgatcgg ggtccagcag
     2761 cgcagcgaac tcgcggaccg tgcgggcgtg cgcatcgtgg gcggtatgcc gcccggcgcg
     2821 gagatgtcga ccacgttcag cggggcggtg ctcaccgcct ccccgcgacc cgaggacgcg
     2881 gaccgggtgc tggctgcgct cgccggcccg acggcatacg aaaccatccg tcgccaccac
     2941 ctgcagcccg cgaccgacct gtagtcagcc gccatcgatg ctcgcgaagt agtcgagtgt
     3001 cgctcgggac gtgctgatca gcggccgcag cgtggcgtcg ccggtgacga cgccgtcggg
     3061 cagatcggcg gcgcgcaact cgcgcccgat gcccgcggcg gcgaccttga gcacctcgac
     3121 gacgcgttgc acgggggcgt cgtcgttggg caccacgaca ctgagggcgg catagaccga
     3181 tccgaggggg ccgagcaccg gcaccgcgac gccgcgggag tcgggatgga tatgcccgtc
     3241 gagcgcggca tacccgcgcc tgcgcaccat ggcgagcaga tcgcgcagcg cccgggcatc
     3301 gggcagcgcg tgcggggtcg gctgcggcca gccggcgcgc acgacgtcct cgaggacgct
     3361 ttcgtcggcg tgggcgagca gcacgacgcc actcgagctc agcggcatcg gcaggcgacc
     3421 gccgatcagc gtggcgttga cgacgggcgt cggggtcgga gagccggtcg atgaacagga
     3481 catcggtgcc gctgcggacg ccgagctggg tgtgctggcg cacgcgcgcc tggacggcct
     3541 tcatccacgg gcgggccagc tcacgcaggc cgagcgcacc cggcgtgcga gaagcgaaat
     3601 cccacaaccg aattccgagc cggaagctgc ggtcgggcag tcgctcgagc agtccttagt
     3661 gggtgagttc cgcgaccagg cggtgggtgg aggagagcgg caacccggcg acctgcgcga
     3721 tctcgctgag tgtcaggaag ggccggcgcg catccaaggc gtccacggtg ccgcgccgac
     3781 atggcctatg tcgacgacct cgccgcctat ccggacaagg tcacggtcaa ctgctccgac
     3841 accgacggcc gcgccgccct cgaccgctgg cgtcctgacg acccgcgagt gcgcgtatac
     3901 gcctgcggca cagcgcgatt gctcgacgac gtctcggcgt ggggtgccgt gaccggcggc
     3961 ttcccgccca agatcgagcg cttcgtcgcc gccaccgcag acgccgggcg cccgcgcgca
     4021 gccttcgagg tccgggctgc gcggtccgac gtcacgacca ccgtggctcc tcacgagacg
     4081 atcgtcgccg ccccccgtcg ggccggcgtc gaggtgatca cctcctgcgc ccagggcgtc
     4141 tgcggcacgt gcgagaccga ggtcctcgag ggcgaaccgg accaccgcga ctccctcctc
     4201 gacgacagcg aacgcgagat cgcccgttgc ctcttcccat gcgtctcccg cagccgcagc
     4261 gagcggctcg tcctcgacct ctgacccgcg gcatacccga aagaacccca catgaccacc
     4321 gcgatcgcct tctgcgagag cgatatcgac cccttcgacg aggaattcct cgccgatccg
     4381 ttgccccgcc tggcacagct gcgcgacctc gggccagtcg tctatctgag ccggtatgac
     4441 gcgcatgccg tcgcgcggta tgcggacgtc catcgcgcgc tgaccgactg gcagtcgctg
     4501 cagtcggggg ccggagtcgg cttgtcgaac ttcaaccacg aggagccgtg gcggccaccg
     4561 agcctgctgc tcgaggccga cccgcccagg cacgacgcgc cccggcacgt gctgcagcag
     4621 atcctctccc cccgacgcct gcgggccctc tcagacaggt ggcgctcgaa tgccgagcag
     4681 gtcgtcgacg acgtcctggg ccgaggagat gaattcgacg ccgtgaccga aatcgccgac
     4741 gtcttcccct ccgggtcttc cccgatgccg tcggtctcgc acgcccaagc cccgctcatg
     4801 gtgcgatcgc tgctgaccgc cggtgtggac accacggtca cgggcatcgc cgccgaacac
     4861 cttgcgcggg tatgaggcgc tacccgtccg cgtcgcccgc gcgtgatcct tccccatcca
     4921 ctctcgatgg ggtggggtct cgccccggac actcagctct tgctgggcca gccggtgtac
     4981 tgctcggcca ggtaggtgcg gccggcgcga gtgttcacga cattgtcgag ttcacccagc
     5041 tgccgcatga ggtcgaattc cagcccgccg ggggcggcgt gcagcatctg ggtcatccaa
     5101 taggagaagt tctgcgcccg ccagacgcgg ccgagagcgc gcggctcgta atccgccagc
     5161 gccgcttcac catcggagcc cagggcgcgg gtcagcacgt ccgccaggat cttcacgtcg
     5221 tgcagggcca ggttgagacc gcgtgcgccg gtcgggggca cggtgtgcgc ggcgtcgccg
     5281 gcgaggagga gcgaacccca ccgcatcggc tgctggacga aggagcggaa gcgcaggacg
     5341 accttctcgg tgatggggcc ctccttgagg gagaaaccgt caccgccggc cacgcgcgcc
     5401 tgcagggtct cccagatccg ctcgtcgctc caagcgttga tgtcctcttc ggggtcgcac
     5461 tggaaataca tgcgctgcaa cgcttccgtg cgctgactga tcagggcgaa gccgtgctcg
     5521 gagtgggcat agaccagctc gggggcgctc atcggcgcct cggtcatgag gccgaaccac
     5581 gcgaacgggt attcgcggaa gaactggcgc ctctccgcct cggggatcaa gtagcggcac
     5641 atgctgcggg agccgtcggc tccgacgaca tagcgggcct gcacctcggt gcggttgccg
     5701 tcagcgccgg tgaaggacat ggtcggccgt tcggtggtct cgccggcgac cgcagggtcg
     5761 gtgacgccga agtggacgcg gccgccgtcc cgagcccgcg cgtcggccag gtcgatgaac
     5821 acgtcggtct gcggatagag ccacaccgat tggccgacca gttccttgaa gttgatgtgg
     5881 tggctctggc cgccgaagcg cagataagtg ccctcgtgct cgtagccgtc gcgcaggatc
     5941 cgatcgctca cgcccgtctc gaccaggtcc cgcgccgcgt ccgcctcgag aatgccggcc
     6001 cggtgggtcg tttcgatctc ctcgcgggtg cgcgtgtcga tgttgaccac gtcgataccc
     6061 gcccggtcga gccagtgcgc gagcatcagg ccggctgggc cggcaccgac gatggcgacc
     6121 tccgccgtct cggtgctgtc gacgggagtg gggacgcttg cgtcggtcat gcctggctcc
     6181 tttgccgagg ggatttcctg ccgttgcgac cactgtgccc gcgcggcata cgacccggcg
     6241 acgccactcc cgtcagacgg aatcatgacc agaaggcgtc ggtcagggtg tcccggcccg
     6301 ttcccccagc gtgcgggaca cccctcgcgc cgccgacacg acggggtggc gcagatgacg
     6361 cacggcggcg ctcgtcgccc cggcgacgat gatgccgagg gcggcctcga cgtcgcctct
     6421 ctcgccgatc cgcaccgggg cggcgacccc gacggtgctg tcctggaact gccggtcgtt
     6481 gacggcgaac gtggctcgcc ggacctgggc cagcatggag cgcagagtgc gcgcatcggt
     6541 gacggtatgc ggtgtgtgcc ggtccatcgg tccggccagg accgcgtcgc gaatctcgct
     6601 gggggcgtat gcgagcagca ccatccccat cgcggtgccg aggaggggaa agcgcagccc
     6661 gaccatggtg cgcaactcca ccgaccggtg tccggcgatg cgctcgacga agaccatctc
     6721 ggtgccctca cgcaccgcga gttggatgtt ctcgtgggtc gcctcgtaaa gatcctgcat
     6781 gaacgggagc gccgcctccc gcaggatctg tccacgcggg cagaccgatc ccacttccca
     6841 caatcgcaac ccgatatgcc agtcgccgtc cgcggtccgc tcgagcgcgc cccaggcggc
     6901 gagttcactg acgatgcggt gggtcgtgct cagggccagc cctgtgcgct tggcgatctg
     6961 gctcagggat tggctgggcc gttctcgcgt gaacgtgcc
//
LOCUS       DAOPJG010000175         6883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_11712_length_6883_cov_7.997510, whole genome shotgun sequence.
ACCESSION   DAOPJG010000175 DAOPJG010000000
VERSION     DAOPJG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11712_length_6883_cov_7.997510"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..980)
                     /locus_tag="PKU75_13605"
     CDS             complement(51..980)
                     /locus_tag="PKU75_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPF81960.1"
                     /translation="MSDKAVNPDERYRKLTIGVCPDQWGVWFPQDPVQIDWRTALQEM
                     RTAGFSIMETGPYGYFPTDPIELKDVMDEHGFRVVAGTGWGILHKAEAWDDTEKLFRA
                     IGQTHAEVGAEYVVHLPPMFRDEKTGDYIDDKVLSPEAWQRYIANANRLGKIMKQDYG
                     LTMVLHPHGDSHIETPEDIDRVFEATDPEYVGFCLDTGHIVYGSGDPMELCRKYPERI
                     SYVHIKAMDPLIVKQAHDEDWPFVKAVKAGCAVAPPLGEPDMGLMIEALADLDKDLYV
                     ICEEDMYGCDKSYPLPNAVKTREYLASLGLGLP"
     gene            1137..2207
                     /locus_tag="PKU75_13610"
     CDS             1137..2207
                     /locus_tag="PKU75_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPF81961.1"
                     /translation="MTATPLEQMTAGDFPTVLWNDSADPQELGQSIAWGAVGATCNPV
                     IAVQCIKNDLPRWTARMSEIAQERPEASESEIGWQVVEEISKEAAAQLKPAFDKHGGR
                     NGRLSMQTDPRNHRNAKALADQAKYFSTLAPNIIVKIPATATGLTAIEDAVSRGVSIN
                     VTVSFTVPQAVAAAEAIERGMAKREAAGHDVSNMGHVVTIMVGRLDDWLKDCAKTEGS
                     DIDPEYLEWAGVAAFKRAYSIWQERGYRSRLLSAAFRNTKQWSEFVGGDVVISPPFAW
                     QEKIQAAGAAPENRMDVPVDPAIVEALSAAFPDFRRAYDPDGMSPEEFDTFGATRKTL
                     RQFLGADEELDQMVRDVLVPAP"
     gene            2240..4174
                     /gene="iolD"
                     /locus_tag="PKU75_13615"
     CDS             2240..4174
                     /gene="iolD"
                     /locus_tag="PKU75_13615"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HPF81962.1"
                     /translation="MTMSETIRLTTAQAIVKFLGQQYSERDGAEQRLVTGMFGIFGHG
                     NVCGVGQALLQDQIAFEDAGADDPLAAGHLPYYLIRNEQSGVHAATAYARSRERLQVM
                     AVTTSIGPGATNMITGAALATTNRIPVLLFPSDQFANRMPDPVLQQLESPQTLDTAVT
                     DCFRPVSRFFDRIVRPEQLMPSLLNAMRVLTDPADTGAVTIALPQDVQAEAYDWPAAF
                     FRKRVWHIARPVPERAALERAVEVLRSAKRPLVVAGGGVVYSGASEQLREFASATGIP
                     VSDTHAGKGAINWDHPSAVGAVGSTGTTAANALARTADVVVGIGTRYSDFTTASHTIF
                     AGDGVEGDVRFVNINTLGFDAAKHSATMVLADAREALTALSAALDGWEVEAAYRQEAA
                     DLDADWQRQVDECYHRDHAPLPAQTEVFGALNELMGPEDVVINAAGSMPGDLQCLWRA
                     TTPVQYHVEYAYSCMGYEIPAALGAKLALGDDHEVVAIVGDGTYQMSPMEIATIVSEG
                     LKVIIVLLQNHGFASIGALSESRGSQRFGTKYRMRSGGAGGRLDGAEVPFDLAASAAS
                     WGADVLRCNGIEEFRANYAKAAAADHTTVLYIETDLMGPNPPGSAWWDVPVAEVSDLE
                     STQAAYAEHVENRKAQRHYL"
     assembly_gap    4260..4359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4377..5261)
                     /locus_tag="PKU75_13620"
     CDS             complement(4377..5261)
                     /locus_tag="PKU75_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPF81963.1"
                     /translation="MGRSLRDRVGGAPISWGVCEVPGWGEMLPPERVLPEMKSVGLTA
                     TELGAPGFFPEDSDGLDATLREHEMSLIGGFVPLVLHDPAQRADALDRAESVAAHFAA
                     HGGTHFVTAVVQDYDWSKPVPLDADGMAELGRGLREVDEVCARHGLTQVLHPHVDTLV
                     ETKADVDLALEHTDVKWCLDTGHLYIGGVDPVAFAKEHGSRVGHAHLKDVDAAVAQRL
                     MGGELTLLQAVQAGLFEPLGRGDVPVGEAISALEEQGYQGWYILEQDCALTDGVPAEG
                     EGPIHDVRTSVAYLDALA"
     gene            5526..6185
                     /locus_tag="PKU75_13625"
     CDS             5526..6185
                     /locus_tag="PKU75_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPF81964.1"
                     /translation="MAPRRQHLLDLAVLGLLHEAPMHGYELRKRLNLALGPFRALSFG
                     TLYPALRGLVADGLISESNPVSAPNPVSASNPATGHAPATPASRRPRIVYELTDAGRT
                     RFAELNNRADDTAYDDDGFEVRIAFFARTESAVRLRILEGRRARLEDRLAALRTAPAT
                     PSAGDIWSRALTRHSEDTLERDLRWLGELIAAERSAPARPGTLTDPSHPAFTPRQTNQ
                     K"
     gene            6197..>6883
                     /locus_tag="PKU75_13630"
     CDS             6197..>6883
                     /locus_tag="PKU75_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPF81965.1"
                     /translation="MGSIRVAIVGVGNCASSLVQGVEYYKDADPAGSVPGLMHVKFGD
                     YHVFDVQFVAAFDVDDKKVGKDLAEAINSSENNTIKICEVPTTGVTVQRGHTLDGLGK
                     YYRLTIDESAAEPVDVVQALKDAEVDVLVSYLPVGSEEADKFYAQCAIDAGVAFVNAL
                     PVFIASDPEWAAKFEHAGVPIIGDDIKSQVGATITHRVMAKLFEDRGVVLDRTYQLNV
                     GGNMDFKNMLE"
BASE COUNT         1150 a         2358 c         2206 g         1069 t
ORIGIN      
        1 gcccgagacg acgatcaacc ccacgccatc agcccatcgg tggatcgagg ctaaggcagg
       61 cccagaccga gggacgccag gtattcccgg gtcttgacgg cgttgggcag gggataggac
      121 ttgtcgcagc cgtacatgtc ttcttcacag atgacgtaga ggtccttgtc gaggtcggcg
      181 agggcctcga tcatcaggcc catgtcgggc tcgccgagtg gcggggcgac ggcgcagccg
      241 gccttgacgg ccttgacgaa gggccagtcc tcgtcgtggg cctgcttgac gatgagcgga
      301 tccatcgcct tgatgtgcac ataggagatg cgctcgggat acttgcggca caactccatc
      361 gggtcgccgg agccgtagac gatgtggccg gtgtcgaggc agaagccgac gtattcgggg
      421 tcggtcgcct cgaagacgcg gtcgatgtcc tcgggggtct cgatgtggct gtcgccgtgc
      481 gggtggagca ccatcgtcag gccgtagtcc tgcttcatga tcttgccgag ccggttggca
      541 ttcgcgatgt aacgctgcca ggcctcgggg gagagcacct tgtcgtcgat gtagtcgccg
      601 gtcttctcgt cgcggaacat cgggggcagg tgcacgacat actcggcgcc gacctcggcg
      661 tgcgtctggc cgatggcccg gaagagcttc tcggtgtcgt cccacgcctc ggccttgtgc
      721 aggatccccc agcccgtgcc ggcgacgacc cggaagccgt gctcgtccat cacgtccttg
      781 agctcgatcg ggtcggtggg gaagtagccg taggggccgg tctccatgat ggagaagccg
      841 gcagtccgca tctcctgcaa cgcagttcgc cagtcgatct gaacggggtc ctgcgggaac
      901 cacacgcccc attggtcggg acacacgccg atggtcagct tgcggtagcg ctcgtcgggg
      961 ttgacggcct tgtcggacat gggcacgcga tcctctcgtc acgaaatgat ctgatgtcct
     1021 atcaaaacac ctgggtcacc aattcacaat gcctctcgat tccccgacag ccctagcgct
     1081 cgcgtcgtat ttgtcctaac attaggtcaa ccgccaccga tgacgaggag cagacgatga
     1141 ccgcgacccc cctggagcag atgaccgccg gcgacttccc caccgtgctg tggaacgact
     1201 cggccgatcc gcaggagctg ggtcagtcca tcgcgtgggg cgccgtgggg gcgacgtgca
     1261 atccggtcat cgccgtgcaa tgcatcaaga acgatctgcc ccggtggacg gctcggatga
     1321 gcgagatcgc ccaggagcga cccgaggcga gtgagagcga gatcggttgg caagtcgtcg
     1381 aggagatctc caaggaggcc gccgcacagc tgaagccggc cttcgacaag cacggcggac
     1441 gcaacgggcg cctctcgatg cagaccgacc cgcgcaacca ccgcaacgcg aaagcccttg
     1501 cggaccaggc caaatacttc tcgacgctgg cgcccaacat cattgtcaag atccccgcga
     1561 cggccacggg cctcacggcc atcgaggacg ccgtgagccg tggagtctcc atcaatgtca
     1621 ccgtgtcctt caccgtcccg caggccgtcg ccgccgccga ggcgatcgaa cgcggcatgg
     1681 ccaagcgcga ggctgccgga cacgacgtgt ccaacatggg ccacgtcgtg accatcatgg
     1741 tcggccggct cgacgactgg ctcaaggact gcgccaagac cgagggcagt gacatcgacc
     1801 ccgagtatct cgagtgggcc ggcgtcgctg cgttcaagcg cgcctacagc atctggcagg
     1861 aacgcggtta ccgctcacgc ctgctctcgg ccgccttccg taacaccaag cagtggtcgg
     1921 agttcgtcgg cggcgatgtc gtcatctccc cgcccttcgc gtggcaggag aagatccagg
     1981 ccgccggggc ggcacccgag aaccgtatgg acgtgccagt cgacccagcc atcgtcgagg
     2041 ccctgtccgc ggccttcccc gacttccgcc gcgcctacga cccggacggc atgagcccgg
     2101 aagagttcga taccttcggt gcgacccgga agacgttgcg gcagttcctc ggtgccgacg
     2161 aggagctgga ccagatggtg cgcgacgtcc tggtgccggc gccctgaccg gcacgcacac
     2221 ggcatacagc aaagacggga tgacgatgag cgagaccatc cgcctgacga cggcgcaggc
     2281 gatcgtgaag ttcctcggcc agcagtattc ggagcgcgac ggggccgaac aacgcctcgt
     2341 cacggggatg ttcgggatct tcgggcacgg caatgtatgc ggcgtcggcc aggcgctcct
     2401 gcaggaccag atcgccttcg aggacgcggg cgcggacgac ccgctggcgg ccggacatct
     2461 gccctactac ctcattcgca acgaacagtc gggcgtgcac gcggccacgg catacgcccg
     2521 tagtcgcgag cgccttcagg tgatggcggt gacgacgtcg atcggcccgg gggcgacgaa
     2581 catgatcacg ggcgccgcgc tcgcgacgac caaccgcatc ccggtgctgc tcttcccgtc
     2641 cgaccagttc gccaaccgga tgccggatcc cgttctgcag caactcgaat cgccacagac
     2701 gctggatacg gcggtcaccg actgcttccg tccggtttcg cgcttcttcg accggatcgt
     2761 ccgccccgag cagctgatgc cctcgctgct caacgcgatg cgcgtcctga ccgaccccgc
     2821 cgacaccgga gccgtgacga tcgcgctgcc gcaggatgtg caagccgagg cgtatgactg
     2881 gccggccgcc ttcttccgca agcgcgtgtg gcatatcgct cgcccggtgc cggagcgggc
     2941 ggcgctcgag cgcgccgtcg aggtgcttcg ctccgcaaag cgtccgctcg tcgtggccgg
     3001 tggcggggtc gtctattcgg gcgcgtccga gcagttgcgc gaattcgcct cggccacagg
     3061 cattcccgtg tcggacaccc acgcgggcaa gggcgcgatc aactgggatc acccctccgc
     3121 agtgggcgcg gtcggctcca ccggcaccac ggccgccaat gcgttggcgc gcaccgcgga
     3181 tgtggttgtc ggcatcggaa cccgttacag cgacttcacc accgcctcgc acacgatctt
     3241 cgcgggcgac ggcgtcgagg gtgacgtgcg cttcgtcaac atcaacacac tcggcttcga
     3301 cgcggccaag cacagcgcca cgatggtgct cgccgacgcg cgcgaggctc tgacagcgct
     3361 ctccgcagcg ctggacgggt gggaggtcga ggcggcatac cgtcaggaag cggctgacct
     3421 cgacgcagac tggcagcgcc aggtcgacga gtgctatcac cgcgaccacg cgccacttcc
     3481 ggcgcagacc gaagtcttcg gtgcgctcaa cgagttgatg ggccccgagg atgtcgtcat
     3541 caacgcggcc ggctcgatgc ccggtgacct ccaatgcctt tggcgcgcaa cgactcccgt
     3601 gcagtatcac gttgagtacg cgtactcgtg catgggatac gagatcccgg ctgccttggg
     3661 tgccaagctc gcgctcggtg acgaccacga ggtggtcgcg atcgtcggtg acggcaccta
     3721 tcagatgtcg cccatggaga ttgcgacgat cgtctccgag ggtctgaagg tcatcatcgt
     3781 gctcctgcag aaccacggct tcgcctcgat cggagcgctg tcggagtccc gcggctcaca
     3841 gcggttcggc acgaagtacc gcatgcgctc gggaggtgcc ggcggccgcc tcgacggcgc
     3901 cgaggtgccc ttcgatctcg cggccagcgc cgcctcatgg ggcgcggatg tgttgcgctg
     3961 caacggcatc gaggagttcc gcgcgaacta cgcgaaggcc gccgcggcgg accacacgac
     4021 ggtcctctat atcgagaccg acctcatggg tcccaacccg cccggctcgg cctggtggga
     4081 cgtgcccgtc gccgaggtga gcgacctgga gtcgacgcag gcggcatacg ccgagcatgt
     4141 ggagaaccgg aaggcccagc gccactacct ctgagccgcc gactcgcccc cctccgaagc
     4201 ggggcctggg tcacgtgtga gtcaggccag cgcgtccagg taggcgaccg acgtgcgcan
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctgggtcacg tgtgagtcag
     4381 gccagcgcgt ccaggtaggc gaccgacgtg cgcacgtcgt ggatcgggcc ctcgccctcg
     4441 gcggggacgc cgtccgtgag agcgcagtcc tgctcgagga tgtaccagcc ctggtaaccc
     4501 tgctcctcga gcgccgagat cgcttctccg acagggacat cgcctctgcc gagcggctcg
     4561 aacaaacccg cctggacggc ctggaggagt gtgagctcgc cgcccatcag ccgctgggcg
     4621 accgcggcgt cgacgtcctt gagatgggcg tgcccgaccc gggagccgtg ttccttggcg
     4681 aacgcgaccg ggtcgacgcc accgatgtag aggtgccccg tgtcgaggca ccacttcacg
     4741 tcggtgtgct cgagcgcgag gtcgacgtcg gccttggtct cgacgagggt gtcgacgtgc
     4801 gggtgcagga cctgggtcag gccgtggcgg gcgcacacct cgtcgacctc ccgcaggccc
     4861 cggcccagct cggccatacc gtcggcgtcc aggggcaccg gcttggacca gtcgtagtcc
     4921 tgcacaaccg cagtgacgaa atgcgtgccg ccgtgcgcgg cgaagtgagc ggcgaccgac
     4981 tcggcgcgat cgagcgcgtc ggcgcgctgc gcggggtcat gcagcaccag cggcacgaac
     5041 cccccgatga gcgacatctc atgctcccga agcgtggcgt ccagcccgtc ggagtcctcg
     5101 gggaagaagc ccggtgcccc gagttcggtc gcggtcagcc ccaccgactt catctcgggc
     5161 agcacacgct cgggcggcag catctcgccc cagccgggaa cctcacagac tccccatgag
     5221 atcggcgccc cgccgacccg gtcacgcagc gaacgaccca tcgcactctc ctttgaactg
     5281 actaaggcca caatttgtcc tgacattcta tcagcccgtt gtatgcagtg acgggccggt
     5341 tcgtcccgca gcgcaggcgc ccggccgccg cacccccgag ggggtcgcac tgacctattc
     5401 gacgggtcga cgtgacgcct cgacggggtg ggaaggacat ctcgacgggt gtgtcggagt
     5461 gacaaagacg ggctttgtcc tgttatgatc ccatgtatcg gatagatata tcgaaggagt
     5521 atgcagtggc gccgcggcga cagcacctgc tggacctggc cgtcctcggg ctgctccacg
     5581 aggctccgat gcacggctat gagctgcgca aacggctcaa cctggcactc ggcccgttcc
     5641 gggctctcag cttcggcacg ctctatccgg cgctgcgcgg cctggtcgcc gacggcctga
     5701 tctccgagtc gaacccggtc tccgcaccga accccgtctc cgcctcgaac ccggcgaccg
     5761 ggcacgcccc ggcgacaccc gcctcccgcc ggccgcgcat cgtctacgaa ctgaccgatg
     5821 ccggccgcac ccggttcgcc gagctcaaca accgggccga cgacacggca tacgacgacg
     5881 acgggttcga ggtgcgcatc gccttcttcg cccgcaccga gtccgcagtc cggttgcgga
     5941 tcctcgaggg ccggcgcgcc cgcctcgagg accggctggc cgccctgcgc acggccccgg
     6001 caaccccgag cgccggcgac atctggagcc gcgccctgac ccggcattcc gaggacactc
     6061 tcgaacgcga cctgcgctgg ctcggcgaac tcatcgccgc cgagcgatcc gcacccgccc
     6121 gcccgggcac cctcaccgac ccctcccatc cggcgttcac cccacgtcag accaaccaga
     6181 aataaggagc accaccatgg gttcgatccg cgtcgccatc gtcggcgtcg gcaactgcgc
     6241 gagctcgctc gtgcagggcg tcgagtacta caaggacgcc gacccggccg gcagcgtgcc
     6301 gggcctgatg cacgtcaagt tcggcgacta ccacgtcttt gacgtgcagt tcgtcgccgc
     6361 cttcgacgtc gacgacaaga aggtcggcaa ggaccttgcc gaggccatca actcgagcga
     6421 gaacaacacg atcaagatct gcgaggttcc caccaccggg gtgaccgtcc agcgcggcca
     6481 caccctcgac gggctgggca agtactaccg cctgaccatc gacgaatcgg ccgccgaacc
     6541 ggtcgacgtc gtccaggccc tcaaggacgc cgaggtcgat gttctcgtct cctacctccc
     6601 ggtcggttcc gaggaggcag acaagttcta cgcccagtgc gccatcgacg ccggcgtcgc
     6661 cttcgtcaac gccctcccgg tcttcatcgc ctccgacccc gagtgggccg cgaaattcga
     6721 gcacgccggc gtgccgatca tcggcgacga catcaagtcc caggtcggcg cgaccatcac
     6781 ccaccgcgtc atggccaagc tgttcgagga ccgcggcgtc gtgctggatc gcacctacca
     6841 gctcaacgtc ggcggcaaca tggacttcaa gaacatgctc gag
//
LOCUS       DAOPJG010000176         6875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_11732_length_6875_cov_7.017742, whole genome shotgun sequence.
ACCESSION   DAOPJG010000176 DAOPJG010000000
VERSION     DAOPJG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11732_length_6875_cov_7.017742"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /gene="cutA"
                     /locus_tag="PKU75_13635"
     CDS             <1..1965
                     /gene="cutA"
                     /locus_tag="PKU75_13635"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HPF81966.1"
                     /translation="DVPGNVSAHLLQEVGDLDAAMAGAPHRLSLDLSIERSASIPMEG
                     KGVYAKWDSDAEHLTLWSSTQTSTGVRAAVATRLGLPLAKVDCITPDVGGGFGSKIMH
                     PWPEEVLVPWAAMILGHDVKWTEDRREHFISSTHERGQLQQVEVGFDDEGRVLALDVK
                     FWHDNGAYTPYGIIVPIITATQLLGPYKPGTYRVEFWSLYTNTVLVTPYRGAGRPQGV
                     FAMERTMDAIADYLGIDRAEVRSRNFILPEEMPYDHGLMFQDGRPLKYDSGDFPASLA
                     KLKALVGWDDFAAYREEAEKHGRRVGLGIGCYVEGTGVGPYEGAHIVIETSGRVNVAT
                     GLTTQGQGHQTAFAQIVADELGVPLESVHVTTGDTRKMAYAVGTFASRAAVMSGNAVA
                     LAARNARAKALKIAADALEVSADDLEIEDGVISVKGAPDTRMDIGMTAVLSNPLRYAF
                     DEAAKAATQFAGTFDPDKPPVADDEEPGLEGKDFYSPTQATFANGMHAAVVETDPVTA
                     EIRILRYCVIHDCGTMINPMIVEGQVHGGVAQGVGGALYERMVYDESGQLLNASFMDF
                     LMPYASEVPHVETDHLETPSPLNPLGIKGAGEAGTIPGSAVIASAIEDAEGFPITRMP
                     ISPVELWELRQRHERGEIPSLRRAHSTTTE"
     gene            1975..2640
                     /locus_tag="PKU75_13640"
     CDS             1975..2640
                     /locus_tag="PKU75_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HPF81967.1"
                     /translation="MKISGAHTLQAPVDQVWAAFMDPAVLARCLPGCETLTETGPDAY
                     AMRITAGVAAVKGTYDGTVAIADKNEPGSFTMKAQGAGGPGTVAADVAVTLAPSAAGG
                     TDLTFDADAIVGGMIGGVGQRMLTGVTKKMAGEFFKAVDADIAGVRPAEPAAVGAPAE
                     RAGELVGAAAGPVSYAGRPAAAAGGGGIGLGENGFALGVAVGGLVALAGVALGAAVRG
                     LGR"
     gene            2637..3569
                     /locus_tag="PKU75_13645"
     CDS             2637..3569
                     /locus_tag="PKU75_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPF81968.1"
                     /translation="MKAFTAAAIQIAPVPGPLTRESIAANVAKCVDFTRECVAATDAE
                     LVVLPESATTGFTPDCSPEELWDLVSELPGGIIQPVQDLAKELGVVICVGTYERGPKR
                     GIVYNSSALIDRTGEVLGVYRKTHPFCSEIITGGGWVTPGDTVAVCDTDLGRIGMIIC
                     FDGDYPELSRIQAVQGAELICRPSALLRSADIWELTSRARAYDNHVYVIGANAVGADP
                     AGVLYFGNSHIVTPIAHIVAKAATHEGWVSARLDPDEAMASLTPGSNIGQAFDHLRDR
                     NLDLIKKHLDDLQAPAKVGFPHKHHAFADTAVEE"
     gene            3769..5910
                     /locus_tag="PKU75_13650"
     CDS             3769..5910
                     /locus_tag="PKU75_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HPF81969.1"
                     /translation="MTETAQRRIRVGIDTGGTFTDVVALDEVTGEMVTTKTPSTPSNP
                     ADGFINGMAKVLEQMGASGEDITAVSHGTTVATNKLLEGKVEALGFITTEGYEFILEI
                     ARQAVPDGYGNSYFWVKPPRIVPADFVKTVGGRLDFHGNEIRPFDAEGARQVARWFKD
                     QGITTIGVCFLHAYAEDAHELAMRAILAEEHPEAVVSISSEVLREYREYERSMTTLVD
                     AAVKPNVSRYVKNIHDRLDDFTRASATQSGGDTGGGAPRGIPFYVMKSNGGVLSADEV
                     VHQPITTVLSGPAAGALGAALIAQRAGFGKVLTCDGGGTSTDVSVVLDGEPTLTTEGT
                     VGAYPSKIPMIDVVTVGAGGGSIAWITPEGTLKVGPQSAGADPGPLCYAKGGTEPTIT
                     DAHVVLGRIPPHLLGGEIPLDVDAARAGIEALAGKLGLDFERCATGILEISAWNQANA
                     LRQVSVKRGLDVRDFMLTTFGGSGSLLSCRLVDILDLAGVVVPPNPGNVSAFGLLTVD
                     VKNDYVQTAVARQSTLDYAAIEHTFAALTERAQAALDKEGFAHETHQYVRTVDLRYFG
                     QAYEVRVHAPEGTVDESYAAGVAAAFHDEHRTLYGYDFRDDPTQEVEWVNLRVSGIGP
                     ITRPTIREIASSDGAPELTERAQKSTRPVCFDADQGYVDSPVYWRPDLRAGDSFSGPA
                     IVEEFGSTVPVHPGFTVRVDSFGNLVITKEA"
     gene            5912..>6875
                     /locus_tag="PKU75_13655"
     CDS             5912..>6875
                     /locus_tag="PKU75_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HPF81970.1"
                     /translation="MCEMCNTAATRINHATPDPDAPNPAGLPTAYEHGNRLTPEGTHV
                     DPIVVEIVEGTLASVEMEVETAIARTSRSPMIRDAHDFRAGIHDRQLRKLTGRSYSAL
                     VHPIARDFPLEEMAEGDVFFHNDVYESEGGIGHLPDLCVTVPVFDKGEVVAFVQAFGH
                     HDDIGGAVPGSMPSGATTVFEEGLSVPPIKLWDKGVPNKAALRIMTRNSRMPDSLAAD
                     LDAECSACLMGARRLSELFQRYGRETVEAAFDAILDKTTETYRREILSKIPTGTYVWE
                     DYAEHDGVDEPKLHTQRITLINEETGGPGNGPRLIIDFTGTAPQA"
BASE COUNT         1144 a         2460 c         2256 g         1015 t
ORIGIN      
        1 gacgtgcccg gcaatgtcag cgcccacctg ctgcaggagg tcggcgatct cgacgccgcg
       61 atggctgggg cgccgcaccg gctcagcctg gacctgtcca tcgagcgcag cgcctccatc
      121 ccgatggagg gcaagggtgt ctatgccaag tgggacagcg acgccgagca cctgacgctc
      181 tggtcctcca cccagacctc caccggcgtg cgcgcggccg tcgcgacccg gctcggtctg
      241 ccgctggcga aggtcgactg catcacgccc gacgtcggtg gcggcttcgg gtccaagatc
      301 atgcatccct ggcccgagga agtcctcgtg ccctgggccg caatgattct cgggcacgac
      361 gtgaagtgga cagaggaccg gcgcgagcac ttcatctcct ccacccacga gcgggggcag
      421 ctgcagcagg tcgaggtcgg cttcgacgac gagggccggg tgctcgcgct ggatgtgaag
      481 ttctggcacg acaacggggc ctacacgcca tacggcatca tcgtcccgat catcaccgcc
      541 acccagctgc tggggcccta caagcccggc acctatcggg tggagttctg gtcgctctac
      601 accaataccg tgctcgtgac cccctatcgg ggggccggcc gcccgcaggg cgtctttgcg
      661 atggagcgga cgatggacgc gatcgccgac tatctcggca tcgatcgcgc cgaggtccgc
      721 agccgcaact tcatcctccc ggaggagatg ccctacgacc acggattgat gttccaggac
      781 ggccggccgc tcaaatacga cagcggcgac ttcccggcct cgctggcgaa gctcaaagcg
      841 ctggtcgggt gggacgactt cgctgcctat cgcgaagaag ccgaaaagca cggccgcaga
      901 gtcggtttgg gcatcggctg ctatgtcgag ggcacgggtg tcgggccgta cgaaggggcc
      961 cacatcgtca tcgagacgag cggtcgtgtc aatgtcgcga ccgggctgac cacgcaaggg
     1021 cagggccacc agaccgcgtt cgcccagatc gtcgccgacg agttgggggt gccactggag
     1081 agcgtccacg tcaccaccgg ggacacccgc aagatggcct acgccgtcgg caccttcgcc
     1141 tcgcgcgcgg ccgtgatgag cggcaacgcc gtcgcgctgg ccgcgcgcaa cgcgcgggcc
     1201 aaggcgctga agatcgccgc cgacgccctc gaggtgtccg ccgacgacct cgagatcgag
     1261 gacggcgtca tctccgtcaa gggcgccccg gacacgcgca tggacatcgg catgacggcc
     1321 gtcctgtcca acccgctgcg ctacgccttc gacgaagcgg ccaaggccgc aacgcagttc
     1381 gccggcacct tcgacccgga caagccgccg gtcgccgacg acgaggagcc cggcctggag
     1441 ggcaaggact tctattcgcc gacccaggcg accttcgcca atggcatgca cgcggccgtc
     1501 gtcgagaccg acccggtcac ggccgagatc cgcatcctgc gctactgcgt catccacgac
     1561 tgcggcacga tgatcaaccc gatgatcgtc gagggccagg tgcacggtgg cgtcgcgcaa
     1621 ggtgtgggcg gagccctcta cgagcgcatg gtctacgacg agtccgggca gcttctcaac
     1681 gccagcttca tggacttcct gatgccgtat gcctccgagg ttccgcacgt cgagaccgat
     1741 cacctcgaga cgccgtcgcc gctgaatccg ttgggcatca agggagccgg cgaggcgggg
     1801 accatcccgg gcagcgcggt catcgcctcg gccatcgagg acgccgaagg cttcccgatc
     1861 acgcgcatgc cgatctcgcc cgtcgaactg tgggagctgc gccagcggca cgagcgcggg
     1921 gagatcccgt cgctgcgtcg cgcccactcc accaccaccg aatagggagc agtcatgaaa
     1981 atctccggag cgcacaccct gcaggcaccc gtcgaccagg tgtgggccgc attcatggac
     2041 ccggccgtcc tggcccggtg cctgcccggc tgcgagaccc tgacggagac cggcccggac
     2101 gcctacgcca tgcggatcac cgccggcgtg gccgcggtca agggcaccta tgacgggacc
     2161 gtcgccatcg ccgacaagaa cgaacccggc tccttcacca tgaaggccca gggcgccggc
     2221 gggccgggca cggtggcggc ggacgtggcg gtcacgctcg ccccgtcggc ggccggcggg
     2281 acggacctga ccttcgacgc cgatgcgatc gtcggcggca tgatcggcgg ggtgggccag
     2341 cgcatgctga ccggcgtcac gaagaagatg gccggcgaat tcttcaaggc cgtcgacgcc
     2401 gatatcgccg gagtgcggcc ggcggaaccg gcggctgtgg gcgccccggc ggagcgggct
     2461 ggtgagctgg tcggtgcggc cgcaggtccg gtgtcgtatg cgggccgccc tgccgccgct
     2521 gccggcggag gtgggatcgg gctgggagag aacggttttg cgctcggggt tgccgtcggt
     2581 ggactcgtcg ccctcgccgg tgtggccctg ggagcggcgg tccgcgggct aggccgatga
     2641 aggccttcac cgcggcggcg atccagatcg ccccggtccc ggggccgctg acgcgggagt
     2701 cgatcgcggc gaatgtcgcc aagtgcgtcg acttcacccg cgagtgcgtc gcggcgaccg
     2761 atgcggagct ggtggtcctg ccggagtcgg ccacgacggg cttcactccc gactgctcac
     2821 ccgaagagct gtgggacctg gtcagtgagc tgccgggcgg gatcatccag ccggtgcagg
     2881 atctggccaa ggaactcggc gtcgtcatct gtgtcggcac ctacgagcgc ggccccaagc
     2941 gggggatcgt ctacaactcc tcggccctga tcgaccggac cggcgaggtg ctcggcgtct
     3001 atcgcaagac ccaccccttc tgctccgaga tcatcaccgg cggcggctgg gtgacgccgg
     3061 gcgacaccgt cgccgtctgt gacaccgacc tcggccggat cggcatgatc atctgcttcg
     3121 acggcgacta ccccgagctc tcgcgcatcc aggccgtcca gggcgccgag ctcatctgcc
     3181 gcccctcggc gttgctgcgc tcggcggaca tctgggagtt gacttcgcgc gcccgggcct
     3241 acgacaacca cgtctacgtc atcggcgcca atgccgtcgg cgccgacccg gccggcgtgc
     3301 tctatttcgg caactcccat atcgtcacgc ccatcgccca catcgtcgcc aaggcggcca
     3361 cccacgaagg ctgggtgagc gcccgcctcg acccggacga ggcgatggcc tccctcaccc
     3421 cgggctcgaa tatcggccag gccttcgacc accttcgcga ccgcaatctc gacctgatca
     3481 agaaacacct cgacgacctc caggcgccgg cgaaggtcgg ctttccccac aagcaccacg
     3541 cctttgccga caccgccgtc gaggagtgat ccgccccggc ccgagtcgag agtgcacgac
     3601 acgccgtcat acccggcgca ttgcggcgtg tcgtgcgctg tcgacgtgaa ggagccgcga
     3661 agcgggggca cgggagggct cgaccggtga ggttcgccgg ggatcgcggc gttcgttgtc
     3721 aggttgtgac aaggcgcgtc gtgccccggg gtcgattgaa tcggggacgt gactgagacg
     3781 gcgcagcggc gcatccgcgt aggaattgac accggcggca cattcaccga cgtggtggcc
     3841 ctggacgagg tcaccggcga gatggtgacc accaagaccc ccagcacccc gagcaatccg
     3901 gccgacggat tcatcaacgg gatggcgaag gtcctggagc agatgggcgc gagcggcgag
     3961 gacatcaccg ccgtcagcca cggcaccacg gtcgcgacga acaaactgct cgaaggcaag
     4021 gtcgaggcgc tcgggttcat caccaccgag ggctacgagt tcatcctcga gatcgcccga
     4081 caggcggtgc cggacggcta cggcaactcc tacttctggg tcaagccccc gcgcatcgtc
     4141 ccggccgact tcgtcaagac cgtcggcggc cgcctcgact tccacggcaa cgagatccgc
     4201 cccttcgacg ccgagggcgc ccgtcaggtc gcccggtggt tcaaggacca gggcatcacc
     4261 acgatcggcg tgtgcttcct gcacgcgtat gccgaggacg ctcacgagct ggcgatgcgc
     4321 gccatcctcg cggaggagca ccccgaggcc gtcgtctcga tctcctcgga ggtgctgcgc
     4381 gagtaccgcg agtacgagcg ctcgatgacc accctcgtcg atgccgcggt caagcccaat
     4441 gtctctcgct acgtgaagaa catccacgac cgtcttgacg acttcacgcg ggcgagcgcg
     4501 acgcagtccg ggggggacac ggggggcgga gccccccgtg ggattccgtt ttatgtcatg
     4561 aagtccaatg gcggagtgct ctcggcggat gaggttgtcc accagccgat tacgacggtc
     4621 ctgtcgggtc cggccgctgg tgccctcggg gccgcgctca tcgcccagcg ggccggcttc
     4681 ggcaaggtgc tgacgtgcga cggcggcggc acgtccacgg acgtctcggt cgtcctcgac
     4741 ggcgaaccaa ccctgacgac cgagggcacg gtcggcgcct acccgagcaa gatcccgatg
     4801 atcgatgtcg tcaccgtcgg tgccggcggt ggctcgatcg cctggatcac ccccgaaggc
     4861 actttgaagg tcggccccca gtcggcgggt gccgatcccg gcccgctgtg ttacgccaag
     4921 ggtggcaccg agccgaccat caccgacgcc cacgtcgtgc tgggccgcat acctccgcac
     4981 ctgctcggcg gcgagatccc gctcgatgtc gacgcggccc gcgccggcat cgaggccctt
     5041 gccggcaagc tcggcctgga cttcgagcgt tgtgccacag gcattctcga gatctcggcc
     5101 tggaaccagg ccaacgcctt gcgtcaggtc agcgtcaagc gcggcctcga cgtgcgcgac
     5161 ttcatgctga cgaccttcgg cggctccgga tcactgctct cgtgccgcct ggtcgacatc
     5221 ctcgacctcg ccggtgtcgt cgtcccgccg aaccccggca acgtcagcgc cttcggcctg
     5281 ctgaccgtcg acgtcaagaa cgactacgtc cagacggccg tcgcccgcca gtccacgctg
     5341 gactatgccg ccatcgagca caccttcgcc gccctgaccg agcgcgccca ggccgcgctc
     5401 gacaaggaag gcttcgcgca cgagacccac cagtacgtgc gcaccgtcga cctgcgctac
     5461 ttcgggcagg cgtatgaggt gcgcgtgcac gcccccgagg gcaccgtcga cgagtcgtat
     5521 gccgcgggcg tggccgccgc tttccacgac gagcaccgca cgctgtatgg ctacgacttc
     5581 cgtgacgacc ccacccagga agtcgagtgg gtcaacctgc gcgtctcggg catcggcccg
     5641 atcacccggc cgacgatccg ggagatcgcc tcctcggacg gcgcgccaga gctgactgag
     5701 agggcccaaa aatcaactcg cccagtgtgt ttcgacgccg accaaggtta tgtcgattcc
     5761 cccgtgtatt ggcggccgga cctgagagca ggggacagct tctcgggccc agcgatcgtc
     5821 gaggagttcg gctccaccgt gccggtccac cccggcttca ccgtccgtgt cgacagcttc
     5881 ggcaacctcg tgatcaccaa ggaggcctga catgtgcgag atgtgcaaca ccgccgccac
     5941 ccgcatcaac cacgcgaccc ccgacccgga cgcgccgaac cccgcgggcc tgcccacggc
     6001 atacgagcac ggcaaccggc tcacccccga gggcacccac gtcgacccca tcgtggtcga
     6061 gatcgtcgag gggaccctcg cgagcgtcga gatggaggtc gagaccgcta tcgcgcgcac
     6121 cagccgcagc ccgatgatcc gcgacgccca cgacttccgc gccggcatcc acgaccgcca
     6181 gctgcggaag ctgaccggcc gctcctatag cgcgctcgtc caccccatcg ctcgtgactt
     6241 cccgctcgag gagatggccg agggcgacgt cttcttccac aacgacgtct acgaatccga
     6301 gggcggcatc gggcacctgc ccgacctgtg tgtgacggtc cccgtcttcg acaagggcga
     6361 ggtcgtcgcg ttcgtgcagg ccttcggcca ccacgacgac atcggtggag ccgtgcccgg
     6421 ctcgatgccc tcgggcgcca ccactgtctt cgaagaaggg ctctccgtcc cgccgatcaa
     6481 gttgtgggac aagggagttc ccaacaaggc cgccctgcgg atcatgaccc gcaacagccg
     6541 gatgcccgac agcctcgccg ccgacctcga cgccgagtgc tccgcctgcc tgatgggtgc
     6601 ccgtcgcctg tccgaactct tccagcgtta cggccgcgag accgtcgagg ccgccttcga
     6661 cgcgatcctc gacaagacca ccgagaccta ccgccgcgag atcctctcca agatccccac
     6721 cggcacctac gtctgggagg actacgccga gcacgacggc gtggacgagc ccaaactgca
     6781 tacgcagcgg atcaccctga tcaacgaaga gaccggggga ccgggcaacg gcccgcgcct
     6841 gatcatcgac ttcaccggca ccgcaccgca ggcca
//
LOCUS       DAOPJG010000177         6782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_11929_length_6782_cov_7.671770, whole genome shotgun sequence.
ACCESSION   DAOPJG010000177 DAOPJG010000000
VERSION     DAOPJG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11929_length_6782_cov_7.671770"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PKU75_13660"
     CDS             <1..1004
                     /locus_tag="PKU75_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPF81971.1"
                     /translation="SHMFITGPDVIKTVTGEEVGFEELGGARTHNTKSGNAHYLGTDE
                     ADAIEYVKALLSYLPSNNLEEPPAYDLENDLAVTDLDLELDTLIPDSANQPYDMKTVI
                     EHVLDDGDFLEVQALFAPNMLVGFGRIDGRSVGVVANQPMQFAGTLDIDASEKAARFV
                     RTCDAFNVPVLTFVDVPGFLPGTDQEWNGIIRRGAKLIYAYAEATVPKVTVITRKAYG
                     GAYDVMGSKHLGADINLSWPTGQIAVMGAQGAVNILYRKEIKAAEAAGDDVVALRQQL
                     ITDYEDTLANPYIAAERGYIDTVISPSNTRMNVTRALRALRNKRESLPPKKHGNIPL"
     gene            1016..1264
                     /locus_tag="PKU75_13665"
     CDS             1016..1264
                     /locus_tag="PKU75_13665"
                     /inference="COORDINATES: protein motif:HMM:NF025192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HPF81972.1"
                     /translation="MTENTQSADGASGPDARPVLRIVHGNPDAAQIAALVAVLSAAGG
                     DEEAAGPRRISQWSSPERAVRAPAHPSRGAWRTSAWAR"
     gene            complement(1341..1976)
                     /locus_tag="PKU75_13670"
     CDS             complement(1341..1976)
                     /locus_tag="PKU75_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPF81973.1"
                     /translation="MTARIHSVNIGAPTVSNTRSRQPTGIVKAPTDRIDVRAPGPVKG
                     HSGVVGDFIGSAKHHGGDGQAVYAVAREELDWWGAELGRELPDGMFGENLTTAGLDVD
                     GALVDEVWRIGTAVLQVTGPRIPCSTFAGRMQEKQWVKRFAARGHTGAYLAVLQAGSI
                     RVGDAVEILERPRHDVTVPVSFRAAMGDSDAKERFRASAAGSPGLRDYILA"
     gene            2041..3714
                     /locus_tag="PKU75_13675"
     CDS             2041..3714
                     /locus_tag="PKU75_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HPF81974.1"
                     /translation="MTRTPTDVDAIAEGHFEAEVALSPITATSLGIPGHDDQLDDFGP
                     DGFAAGSALRRETLARLAAATPSDDIDAVTIAAMRERLGVAEEIYAAGLEDSALNVLA
                     SPLQGIRGIFDLMPTDTDREWSTFGARLSAVPAALDQWVTTLRAAAARGQVAPHRQVA
                     ACIGQCAEFVSDDGYFAGLRNGLAAAGGLDDAVRARVGEGIDAAATAYDALGRTLRET
                     LLDKAPEVDACGIDTYRLHSRYFLGSEVDLAETYDWGQEELARIEAQQTEVADQVQAG
                     ATLEEAIDILDADPAYQLHGTEALQVWMQAKADDVIDALADVHFDIPGPVRVIEGCIA
                     PTQTGGIYYTGPSDDFSRPGRMWWSVPRGVTEFTTWRELTTVYHEGVPGHHLQVAQTV
                     YRRELLNSWRRLSAWTSGHGEGWALYAERLMAELGFMDDPGNRLGWLDGQSLRAARVV
                     VDIGVHCGFAAPAEVGGGAWDYDKAWRFLRAHTQMEEAMLRFELDRYLGWPGQAASYK
                     IGERLWLQLREESQQRAGKAFDLKEFHRRALDIGGVGLDTLRAAVLGEL"
     gene            3872..5767
                     /locus_tag="PKU75_13680"
     CDS             3872..5767
                     /locus_tag="PKU75_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HPF81975.1"
                     /translation="MRRVDLVLTLVAVAAAVILFARLSEPLGIPSPLTLLGVGVAASF
                     IPGIPELELSPDVVLFGLLPPLLYAAALATSLVDLRRLITPILGLSVGLVLFTALGVA
                     VVASWLLPIDFALAFALGAIVAPPDAVAATAVARRIGLPRRMTTILEGESLLNDATAL
                     VSLRTALGAAGLAAHGSGSQAGHEEIGFLDVAQDFGIAVFGGIAIGWLVFVVVGIVRR
                     NLTEPPADTALSFVVPFLAYVAAEQIHASGVLAVVTAGLFLAHQAPVLQSASSRLSER
                     VNWASITFLLENAVFLLIGLQMQSIIRDVVDSELTLARSVLIGLAVLAATLLLRPLWV
                     FPFSAIFSRAEFTPSERRRASAVSSWAGMRGVVTLAAALTLPEDTPLRPVLVLIALIV
                     TVGTLVLQGFTLPGLARRLDVRGPDPREDALVEATVVGAATGAGLRALESDENADPDV
                     VARIREQSTSRVNRIWEQLGGDGAETPSEAYRRLRLSMIGAERAELLRMRDADEVDQH
                     VLARVLNGMDAEEAALAYGQQRSGRLRETPLRPPDAVAAACEHLKAQPACVVPDTADG
                     CHRCLEAGMQWVHLRMCTACGHVGCCDSSPGKHASAHFHDTGHPVMRSLEPGEAWRWC
                     FVDEALG"
     gene            complement(5786..6658)
                     /locus_tag="PKU75_13685"
     CDS             complement(5786..6658)
                     /locus_tag="PKU75_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81976.1"
                     /translation="MGSHLQVHELWVGPVVLLTSIAALATLAALSGLVSMATVAITPL
                     GVTNRVTPARLSLLRVGATGLLMAAWVAVSGGMGQVGIGVLAAALVAAVASINLIGPW
                     ALMVFGRMVARLSRRPATMLAARRIVDDPRSAWRTTSTMALGILLATLATVTDGIETE
                     PGDKLPYLAEDMGTGALVTLGIVSVIAATSAGVVQAARVLDQAPQYRALAYAGTDLAT
                     LHRARTLEVGLPLLITMGLTLGFSLLLFASLASFVGTGMVVRFAVAVGLSVTLLFAGL
                     TASRGLLRSAAALD"
BASE COUNT         1028 a         2499 c         2249 g         1006 t
ORIGIN      
        1 cctcgcacat gttcatcacc ggcccggacg tgatcaagac cgtcaccggc gaggaggtcg
       61 gcttcgagga gttgggcggc gcgcgcacgc acaacaccaa gtcgggcaat gcccactacc
      121 tcggcaccga cgaggccgac gcgatcgaat acgtcaaggc cctgctgagc tacctgccgt
      181 cgaacaacct cgaggagccc ccggcctacg acctcgagaa cgacctggcg gtcaccgacc
      241 tcgacctcga actggacacg ctgatcccgg actccgcgaa ccagccctac gacatgaaga
      301 ccgtcatcga gcacgtcctc gacgacggcg atttcctcga ggttcaggca ctcttcgcgc
      361 ccaacatgct cgtcgggttc gggcggatcg acggccgcag cgtcggcgtc gtcgccaacc
      421 agccgatgca gttcgccggc accctcgaca tcgacgcctc cgagaaggcc gcacgtttcg
      481 tgcgcacgtg cgacgccttc aacgtgccgg tgctgacgtt cgtcgacgtg cccggcttcc
      541 tgcccggcac cgaccaggag tggaacggca tcatccgccg cggcgcgaag ttgatctacg
      601 cctacgccga ggccaccgtc cccaaggtca ccgtcatcac ccgcaaggcc tatggcgggg
      661 cctacgacgt catgggatcg aagcacctcg gtgcggacat caacctgtcg tggccgaccg
      721 gccagatcgc cgtcatgggc gcccagggtg cggtgaacat cctctaccgc aaggagatca
      781 aggccgccga ggccgccggt gacgatgtcg tcgcgctgcg tcagcagttg atcaccgact
      841 acgaggacac tctcgccaac ccctatatcg ccgccgagcg cgggtatatc gacaccgtca
      901 tctccccctc caacacgcgg atgaacgtca cgcgagcttt gcgggccctg cgcaacaagc
      961 gcgagtccct gccgcccaag aagcacggca acattcccct gtgatggatc agcagatgac
     1021 cgagaacacc cagagcgcgg acggtgcgtc cggcccggac gcgcggccgg tcctgcggat
     1081 cgtgcacggc aaccccgatg cggcgcagat cgcggcgctc gtggcggtgc tgtccgccgc
     1141 cggtggcgac gaggaagcgg ccgggccgcg gcggatcagc caatggtcca gtcccgagcg
     1201 ggccgtccgg gcgcccgcgc acccgagccg cggtgcctgg cgcaccagcg cgtgggccag
     1261 atgaccccac aaggttcccg ccaccatgtc ccaacggctg gcgccgctgt gcggcgctgt
     1321 ttgacgggct cgaacctgct ctaggccagg atgtagtcgc gcagacccgg cgagccggcg
     1381 gcggaggcgc ggaagcgttc cttggcgtcg ctgtcaccca tggccgcgcg gaagctcacc
     1441 ggcacggtga cgtcgtgacg gggacgctcg aggatctcga cggcgtcacc gacgcggatc
     1501 gaaccagcct gcagcacagc gagataggcc ccggtatggc cgcgcgccgc gaatcgcttc
     1561 acccactgtt tctcctgcat ccggccggcg aaggtcgagc acgggattcg cgggccggtc
     1621 acctgcagca ccgcggtgcc gattcgccac acctcgtcca ccagcgcgcc atcgacgtcc
     1681 agtcccgccg tggtcaggtt ctctccgaac atcccgtcgg gcagctcgcg gcccagctcc
     1741 gcgccccacc agtcgagttc ctcgcgggcc acggcgtaca ccgcctggcc gtcgccgccg
     1801 tgatgcttgg cgctgccgat gaagtctccg acgacaccgc tgtgcccctt gaccggcccg
     1861 ggggcgcgca cgtcgatgcg gtcggtgggc gccttcacga tgccggtcgg ttgccgtgac
     1921 cgagtgttcg agaccgtggg tgccccgata ttgaccgagt ggatgcgcgc cgtcacaggc
     1981 caaccctatg gcagcgattc agcccgcatc cggcagtccg gtcgggtagc ctctcgcccc
     2041 gtgaccagga cacctaccga tgtcgacgcc attgcggagg gacacttcga agccgaggtc
     2101 gccctctccc ccatcacggc cacaagcctg gggatccccg gacacgacga ccaactcgac
     2161 gacttcggtc ccgatggttt cgcggccggc tccgcgttgc ggcgagaaac cctggcccgc
     2221 ctcgccgcgg ccaccccctc cgacgacatc gacgcggtga cgatcgccgc gatgcgcgag
     2281 cgactcggcg tagcggagga gatctacgct gcgggacttg aggattcggc gctcaacgtc
     2341 ctcgcgtcgc ccctgcaggg catccgcggg atcttcgatc tgatgccgac cgacaccgac
     2401 cgcgaatgga gcacgttcgg cgcccgcttg agcgccgtgc cggcggccct cgaccagtgg
     2461 gtcaccacct tgcgggccgc ggccgcgcgg ggacaggtgg cgccgcaccg ccaggttgcg
     2521 gcctgcatcg gtcaatgcgc cgaattcgtt tctgacgacg gctatttcgc cggcttgcgc
     2581 aacggactgg ccgccgccgg cggcctcgac gacgcggtgc gtgcccgcgt cggcgagggc
     2641 atcgacgcgg cggccacggc atacgatgca ctgggccgga cactgcgcga gaccctcctc
     2701 gacaaggcac ccgaggtcga cgcctgcggc atcgacacct accggctgca ctcccgttac
     2761 ttcctcggca gcgaggtcga tctcgccgag acctacgact gggggcagga ggaactcgcc
     2821 cgcatcgagg cgcagcagac cgaggtggcg gaccaggtcc aggccggcgc cacgctcgag
     2881 gaggcgatcg acatcctcga cgccgacccg gcataccaac tgcacggcac cgaggccctg
     2941 caggtatgga tgcaggccaa ggcggacgac gtcatcgacg ccctcgccga cgtgcacttc
     3001 gacattcccg ggccggtgcg cgtcatcgaa ggctgcatcg ccccgaccca gaccggcggc
     3061 atctactaca ccggccccag cgacgacttc agccgtcccg ggcgcatgtg gtggtcggtg
     3121 cctcgcggcg tcaccgagtt caccacctgg cgcgagctga cgaccgtcta tcacgagggc
     3181 gtgcccggcc atcatctgca ggtcgcgcag accgtctatc gccgcgagct gctcaacagc
     3241 tggcgccgcc tgtccgcatg gacctcgggc catggcgagg gctgggctct ctacgccgag
     3301 cgcctcatgg ccgaactcgg cttcatggac gaccccggca accgcctcgg ctggctcgac
     3361 ggtcagtccc tgcgcgccgc ccgcgtggtc gtcgacatcg gcgtccactg cggcttcgcc
     3421 gcgcccgccg aagtcggcgg cggcgcctgg gactacgaca aggcctggcg tttcctgcgc
     3481 gcccacaccc agatggaaga ggcgatgctc cgcttcgaac tggaccgcta cctcggctgg
     3541 cccggccagg ccgcgtccta caagatcggc gagcgcctct ggctccaact gcgcgaagaa
     3601 tcccagcaac gcgccggaaa ggcgttcgac ctcaaggaat tccaccgccg ggccctcgac
     3661 atcggcggcg tcggcctcga taccctgcgc gccgccgtcc tcggggagct ctgactctcc
     3721 ccctgcgtgt tggctccccg ctctccggtc acccaatccg cctctccggt cactcgccca
     3781 ctctctcgga cggaggcgct ttaccgcctg agctcctggg ttgggggctc cggccggctg
     3841 acggacaccg catgcgcccg ggcgggggag aatgcgtcgc gtggatctcg tcctgaccct
     3901 ggtcgccgtc gcggccgccg tcatactctt cgcgcggctg agcgagccgc tcggcatacc
     3961 ctccccactc accctcctcg gtgtcggcgt agccgcctcg ttcatcccgg ggattcccga
     4021 gctggagctc tcgccggatg tggtgctctt cgggttgctg ccgccgctgc tgtatgccgc
     4081 cgccctcgcc acctcgctcg tggacctgcg ccgcctgatc actccgatcc tcgggttgtc
     4141 cgtcggcctg gtgctcttca ccgcgctcgg ggtcgcggtg gtcgccagct ggttgttgcc
     4201 catcgacttc gcgctcgcct tcgcgctcgg tgccatcgtc gcgccgccgg atgccgtggc
     4261 ggcgacggcg gtggcccggc gcatcgggct gccgcggcgg atgacgacca tcctcgaggg
     4321 cgagtcactc ctcaacgacg cgacggccct ggtgtccctg cgcacggcgc tgggcgccgc
     4381 ggggctcgct gcccacggat cgggcagcca ggcaggtcat gaggagatcg gattcctcga
     4441 cgtggcccag gatttcggca tcgccgtctt cggcggcatc gcgatcggct ggctggtctt
     4501 cgtggtcgtg gggatcgtcc ggcgcaatct cacggagccg cccgccgaca cggccctgtc
     4561 cttcgtcgtg cccttcctgg cctatgtggc ggccgagcag atccatgcct ccggagtgct
     4621 cgccgtcgtc acggcgggtc tgtttctcgc ccaccaggcg ccggtcctgc aaagtgcctc
     4681 ttcgcgcctg tccgagcgcg tcaactgggc gagcatcacg ttcctgctcg agaacgccgt
     4741 cttcctcctc atcggcctgc agatgcagtc gatcatccgc gacgtcgtcg acagcgagct
     4801 gaccctggcg cgcagtgtcc tgatcggtct cgccgtcctc gcggcgacgc tcctcctgcg
     4861 cccgctgtgg gtcttcccct tctcggccat cttcagccga gccgagttca ccccctccga
     4921 acgccggcgc gccagcgccg tcagctcgtg ggcgggtatg cgcggcgtgg tcaccctcgc
     4981 cgccgcgctc accttgccgg aggacacgcc gctacgtccc gtgctggtgc tcatcgcgct
     5041 gatcgtcacg gtgggaaccc ttgtcctgca agggttcacc ctgccgggct tggctcgccg
     5101 cctggacgtg cggggccccg atccgcggga ggacgcgctc gtcgaggcca ccgtggtcgg
     5161 cgccgcgacc ggcgccggac tgcgcgccct cgagagcgac gagaacgccg accccgacgt
     5221 ggtcgcccgg atccgcgaac agtcgacgag tcgcgtcaac cgcatctggg aacagctcgg
     5281 cggcgacggc gcggagacgc cgagcgaggc ataccgccgc ctgcgcctgt cgatgatcgg
     5341 cgccgaacgc gccgaactgc tgcgcatgcg tgacgccgac gaggtcgacc agcatgtcct
     5401 cgcgcgggtg ctcaacggga tggacgccga ggaggcggca ctggcatacg ggcagcaacg
     5461 ctcggggcgc ctgcgggaga ctccgctgcg tccgccggac gccgtggcgg ccgcctgcga
     5521 gcacctgaag gcgcagccgg cctgcgtggt gcccgacacc gccgacggct gtcaccgctg
     5581 cctcgaggcg ggtatgcagt gggtccacct gcgcatgtgc accgcgtgcg ggcacgtcgg
     5641 ctgctgcgac tcctcccccg gcaagcacgc cagcgcgcat ttccacgaca ccggccatcc
     5701 cgtcatgcgc tcactcgagc cgggcgaggc gtggcgctgg tgcttcgtcg acgaggcact
     5761 cggctgagaa cttcgtgggt aaaactcagt ccaaggcggc ggcactgcgc agcagccccc
     5821 gcgaagccgt caggccggcg aagaggaggg tcaccgaaag gccgacggcc accgcgaagc
     5881 gcaccaccat gccggttccg acgaaggatg cgagagaggc gaagagcagc aacgagaagc
     5941 ccagggtcag gcccatcgtg atcagcagcg gcaggccgac ctcgagcgtc cgcgcccggt
     6001 gcagggtcgc gagatccgtt ccggcatagg ccagagcgcg gtactgcggt gcctggtcga
     6061 gcacccgggc ggcttggacg acaccggccg aggtcgcggc gatgacggac acgatcccca
     6121 gcgtgacgag ggcaccggtg cccatgtcct cggccagata cggcagtttg tcgccgggct
     6181 ctgtttcgat cccgtcggtg acggtggcca gagtcgcgag gaggatgccc agcgccatcg
     6241 tgctcgtcgt gcgccacgcg ctgcgcgggt cgtcgacgat ccggcgcgcg gccagcatcg
     6301 tcgcggggcg ccgcgacagg cgcgccacca tccggccgaa gaccatcaac gcccatggcc
     6361 cgatcaggtt gatcgaggcg acggccgcga cgagcgcggc cgcgagcacg ccgataccga
     6421 cctggcccat gccgcccgac acggccaccc acgcagccat gaggaggccg gtcgcgccga
     6481 cccgcagcag gctcaatcgg gccggcgtca cccggttcgt caccccgagc ggcgtgatgg
     6541 cgactgtcgc catgctcacc agcccggaga gcgcagccag ggtggcgagg gcggcaatgg
     6601 acgtgaggag gacgaccggc cccacccaga gttcgtggac ctgcaaatgg ctgcccaaga
     6661 acgggattcg agccaacaac ggcaacgagg caacgtatgc cgccgtcccc agtcccgcgc
     6721 ccgcgagcgg agaggctgtg cgcgacgatg tcgtgcatct cccgggcgat ccgcgcgcgc
     6781 tc
//
LOCUS       DAOPJG010000178         6741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_12017_length_6741_cov_7.411008, whole genome shotgun sequence.
ACCESSION   DAOPJG010000178 DAOPJG010000000
VERSION     DAOPJG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12017_length_6741_cov_7.411008"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1769)
                     /locus_tag="PKU75_13690"
     CDS             complement(213..1769)
                     /locus_tag="PKU75_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPF81977.1"
                     /translation="MAQLPSPGAAAIGVGSATFALGAGHLVAAAVAPPASPAVAVAST
                     LVDRAPRAVKEWAISTLGTADKPALVAGVVLVVLALGALAGVLQPRHRLGTWLFVALG
                     VLSAAAALTRPDAGPVSALPTLAAVAAGSLTLRTILPSRSRFDPVEMSIRGRRDVNPP
                     AQPPSDPVRRTLLTAGALTAAGGGLAYLGMRLGAGTAGSATPFALPAPARPLPPLPAG
                     LEATVPGLSALRTPVADFYRVDTALLLPRIDAADWRLRIDGDVDRHIELTFDDLLAMP
                     MVEADITLNCVSNPVGGEYIGSTRWLGVLTRDVLARAGVRATADQILSTSQDGMTIST
                     PVAALTDARGALLAVAMDGKPLTREHGYPVRMVTPGLYGYVGATKWLTRLTATTYAAE
                     KAYWSTRGWAEQAEVKTAARIDVPRRGETVPEGQVVVAGVAWSQAHQGISRVEVRVDE
                     GPWQEAELGPDVGGHYWRQWRWTWEAHTGGRRFTVRATDGEGTIQSGEIAEPAPDGAS
                     GWHGIAVTVR"
     gene            complement(1901..2668)
                     /locus_tag="PKU75_13695"
     CDS             complement(1901..2668)
                     /locus_tag="PKU75_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF81978.1"
                     /translation="MTGPTTTATVPRAAARGIRIRRQAGFEARTMLRNGEQLLVAFLL
                     PAMAMVGLKFASVPDLPAPRIDAIVPGVLALAVVSTAFTSQAIATAFDRRNGVLRLLG
                     TTPLGRDGLLGGKILATFAVLAIHLIALSVLGLALGWRPAGSGIAPALITTVIGAACF
                     VAIAMLVAGRLRAEAVLAMANLLWVLFLGLGLLLPTTTLPNAAGGLAKVLPSGALGDA
                     LRAAFVHGSWPVGQWLVLLAWGGVCGFLATRVFRWSD"
     gene            complement(2665..3375)
                     /locus_tag="PKU75_13700"
     CDS             complement(2665..3375)
                     /locus_tag="PKU75_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF81979.1"
                     /translation="MVPAVEVRDLVKSFGATRALDGVTWSAQSGRVTAVLGPNGAGKS
                     TTIECLQGLQRPDSGTAHVLGEDPWGASAEHRARVGLMLQDGGLPSMSTPPTLLTHLA
                     ALYAAPADPVALAQRLGIDGFARTIIRRLSGGQRQRVALAAALIGRPDVLFLDEPTAG
                     LDPHTRLDVWDIVREQADAGAAVVLATHSFEEADRVADDIVIMDTGRVRAFGGRAEVS
                     GAKSLEDVYFSLTRGGKR"
     gene            3419..4042
                     /locus_tag="PKU75_13705"
     CDS             3419..4042
                     /locus_tag="PKU75_13705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF81980.1"
                     /translation="MCFDERAATWDDDPDKVARSAEVAAAIRRQVDLGDRPRTLEVGA
                     GTGLLSRALRADLGDVVLSDSSNGMIEVAERVIAAERLTGWSTAYANVDRGELPAGPF
                     DLVLSQLALHHMAEPESVLRLIHAALNPGGHLAIADLDHDPDGAFHKAHGDFHGHDGF
                     DRVRLTHWLTELRFADIQISTATTLTKDVDGEPREFPIFLATARKPG"
     gene            4071..5285
                     /locus_tag="PKU75_13710"
     CDS             4071..5285
                     /locus_tag="PKU75_13710"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPF81981.1"
                     /translation="MRRIIRATATLALLTLGTACSGGSGESSGTSAPASGSAASASGT
                     AASATTGGASAAPFQITEVAAYDEPWAMTFLPGTPYAAITQRGGQLIVRDMEAGTDLD
                     VDNEPEVAAGGQGGLGDIIAAPSYEQDQRIYLSWVEPGDNGTRGAVVGNGRLVIDDVS
                     ARIENLNVIWRQVPKVEGEGHFGHRLAMSPDGTYLFVSSGERQQFDPAQDPGTNLGKI
                     LRLNPDGSPAQGNPLSDQEYPSDEIWSWGHRNPLGLAFDAAGNLWESEMGPKGGDEIN
                     LIRAGANYGWPKASNGSHYSGEDIPDHAEGDGYEAPKVFWNPSISPGGLMIYSGSAFP
                     EWVGDAFVPALSGQSLIRVDIDGTSATAADQWSDGPLGERLREVEQGPDGTIWVLQDG
                     GGGKLLHLTPPA"
     gene            5345..6064
                     /locus_tag="PKU75_13715"
     CDS             5345..6064
                     /locus_tag="PKU75_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPF81982.1"
                     /translation="MNISRASLATADAAPESRTRERVLRAVSQQGPITTTALAESLEL
                     SDTAIRRHVDNLVTDGLIEAHDGANPRRGRGRPAKSYVVSEAGHGALTSDYDHLAGQA
                     LRYLAETMGPQAVEDFARQRIADWEVRYATQLAPDLTPSERAEALVAALTRDGFDASL
                     RHVGGPGTPSALHGIQLCQGHCPVRGVAAQFPQLCEAETEAFSRLLGVHVQRLATLAH
                     GDHVCTTFIPTPTIPHQSEAS"
     gene            6061..>6741
                     /locus_tag="PKU75_13720"
     CDS             6061..>6741
                     /locus_tag="PKU75_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPF81983.1"
                     /translation="MTQSIEDLNPGLKGLGHYEYGWADSDSAGASARRGLNTDVVEDI
                     SRRKSEPEWMRELRLKSLRLFDKKPMPTWGSDLTGIDFQNIKYFVKSTEKQATSWEDL
                     PQDIKNTYDRLGIPEAEKQRLVAGVAAQYESEVVYHQIREDLEEKGVIFVDTDTGLRE
                     HEDLFREYFGSVIPPGDNKFASLNTAVWSGGSFIYVPPGVHVDIPLQAYFRINTENMG
                     QFERTLIIA"
BASE COUNT         1126 a         2325 c         2347 g          943 t
ORIGIN      
        1 gccccgagag cagtgccgcc gcggtggctt gccttcttat cctcatacga attcccctgt
       61 ctccgcgtgt atctcgcgct ggtgtcggct gccccccagc cgtgtcggca ccctaagcgc
      121 accggccgac cctgaggaag gcttcggggt gatttggtgg gagagttgag tagaaccgct
      181 catcggccgc gtgcggtgat gtcgcgggcg cctcagcgga cggtgacagc gatgccatgc
      241 caaccgctcg ccccatcagg ggcgggttcg gcgatctcac cggattggat cgtgccctcg
      301 ccgtccgtgg cgcgcaccgt gaatcggcga ccaccggtgt gtgcttccca ggtccagcgc
      361 cactgccgcc agtagtggcc gccgacgtcg ggaccgagct cggcttcctg ccatgggccc
      421 tcgtcgacac ggacctcgac gcgcgaaatc ccttggtgcg cttgggacca tgcgacaccc
      481 gcgaccacga cctggccctc gggcacagtc tcgccgcgcc gtggcacgtc gatgcgggca
      541 gccgtcttga cctccgcctg ttcggcccac ccgcgcgtgc tccagtaggc cttctcggcg
      601 gcatacgtcg tcgcggtgag acgggtgagc cacttggtgg caccgacata cccatacaac
      661 cccggcgtca ccatgcgcac ggggtagccg tgttcgcggg tgagcggctt gccgtccatc
      721 gcgacggcga gcaacgcgcc gcgcgcgtcg gtgagcgcag cgacgggggt ggagatcgtc
      781 ataccgtcct gcgatgtgct gaggatctga tcggcggtgg cgcgaactcc ggcgcgtgcc
      841 aacacatctc gcgtcagcac ccctaaccag cgcgtggacc cgatgtattc gcccccgacc
      901 ggattgctga cgcagttgag ggtgatgtcg gcttcgacca tcggcatcgc gagcaggtcg
      961 tcgaaggtca gctcgatgtg gcggtccacg tcaccgtcga tgcgcagccg ccagtcggcg
     1021 gcgtcgatgc gtggcaacaa gagcgcggtg tcgacgcgat agaagtcggc gaccggggtg
     1081 cgcagcgcgc tgagcccggg gaccgtcgcc tccaagcccg ccggcagggg cgggagtggg
     1141 cgggccggtg ccggcagggc gaatggcgtt gcgctgcctg cggttccagc gccgagccgc
     1201 atcccgaggt atgccagtcc accgcctgcc gcggtcaacg cgccggccgt gaggagggtc
     1261 cgtcgcactg ggtcggaggg tggctgcgcc ggaggattga catctcgacg accgcgaatc
     1321 gacatctcga cggggtcgaa ccgagatctc gacgggagga tggtgcggag ggtgagggag
     1381 ccggcggcga cggcggcgag ggtggggagg gcggagaccg ggccggcgtc cgggcgggtc
     1441 aaggcggccg ctgcggagag gacgcccagg gcgacgaaga gccacgtgcc caggcggtgc
     1501 cggggctgca gcacaccggc gagcgcaccg agggcgagca cgaccaggac gacgccggcg
     1561 acgagcgcgg gcttgtcggc cgtgccgagt gtcgagatcg cccactcctt gacggctcgg
     1621 ggcgcccggt cgaccagggt ggaggccacc gcaacggcgg gcgatgccgg cggcgcgacg
     1681 gcggcggcca ccaggtgacc cgcgcccagg gcgaaagtgg ccgagccgac cccgatggcg
     1741 gctgccccgg gggaggggag ctgcgccatt ggtgcctcct gggagccggc gtggtcggat
     1801 cggttgccga acacggtaaa ggcgatcgga gcgcagcgca gggtttcggg atcttacgga
     1861 gtcatgacga tttgaccgcg cggcgggcgc gcccggcgcg tcagtcggac cagcggaaga
     1921 cacgcgtggc gaggaacccg cagacgccgc cccacgcgag caggaccagc cactgaccaa
     1981 ccggccacga cccgtgcacg aaggccgctc gcagggcatc gccgagggca cccgatggga
     2041 gcaccttggc gaggccacca gcggcgttgg gaagggtcgt cgtgggcagc aggaggccga
     2101 ggccgaggaa gagcacccac agcagattcg ccatggcgag aacggcttcg gcccgcaacc
     2161 ggccggccac cagcatcgcg atcgcgacga agcatgcagc gccgatgacg gtggtgatca
     2221 gggccggcgc gatgccggat cctgccggtc gccagcccag ggccaggccc aggacgctca
     2281 gcgcgatgag gtggatcgcg aggacggcga aggtggcgag aatcttgcca ccgaggagtc
     2341 cgtcgcggcc gagcggcgtc gtgccgagca gacgcagcac cccattgcgg cgatcgaatg
     2401 ccgtggcaat cgcctggctg gtaaaggcgg tggagacgac ggccagggcg agcacaccgg
     2461 gcacgatcgc gtcgatccgt ggagcgggca ggtcagggac ggacgcgaac ttcaggccga
     2521 ccatcgccat ggccggcagc aggaaggcga cgagcagttg ctcgccgttg cgcagcatcg
     2581 tgcgggcctc gaaccccgcc tgccggcgga tgcgtatgcc gcgcgccgcg gcccgcggaa
     2641 ccgtcgccgt ggtcgtcggc ccggtcatcg cttgcctccg cgggtgaggg agaagtacac
     2701 gtcctcgagg gatttcgccc cggacacctc ggcccggcca ccgaacgccc gtacccggcc
     2761 ggtgtccatg atgacgatgt cgtcggcgac cctgtccgcc tcctcgaagg agtgcgtggc
     2821 caagaccaca gcggcgccgg cgtcggcctg ttcgcgcacg atgtcccaca catcgagccg
     2881 ggtatgggga tcgaggccgg cggtcggctc gtccaggaag aggacgtcgg gccgcccgat
     2941 cagggccgcg gccagggcaa cgcgttggcg ttgcccgccc gacagccgcc ggatgatggt
     3001 gcgagcgaag ccgtcgatcc cgagtcgctg agccagcgcg acgggatcgg cgggcgcggc
     3061 atacagcgcg gcaaggtgcg tcaaaagcgt tgggggagtg gacatcgacg gcagaccgcc
     3121 gtcctgcagc atcaacccca cacgggcgcg atgctccgcg gaagcacccc agggatcctc
     3181 gcccagcacg tgcgccgtgc ccgaatccgg ccgctgcaac ccctgcaagc actcgatcgt
     3241 cgtcgacttg cccgcgccat tagggccgag cacagccgtc acccgccccg actgcgccga
     3301 ccaggtgacc ccgtcgagcg ctcgagtggc cccgaaagac ttcaccagat cgcggacctc
     3361 gactgcgggc accaccgcag tctaaagcgc ccccacgggg tgacctaatg tgctgcccat
     3421 gtgtttcgac gaacgcgcgg cgacctggga cgacgacccg gacaaggtgg cgcgtagcgc
     3481 cgaggtggcg gccgcgatcc ggcgccaggt cgatcttggc gaccggccac gcacgctgga
     3541 ggtgggcgcc ggcaccggac tcctctctcg ggccctacgg gccgacctcg gggatgtcgt
     3601 gctctccgac tcctcgaacg gcatgatcga ggtcgccgag cgggtgatcg ccgccgagcg
     3661 cctgaccggg tggagcacgg catacgcgaa cgtcgaccgc ggcgagctgc ccgccggccc
     3721 cttcgacctc gtgctcagtc aactggcgct gcaccatatg gccgagccgg aatcggtgct
     3781 ccggctgatc cacgctgccc tcaaccccgg cggtcatctc gccatcgccg acctcgacca
     3841 cgatcccgat ggcgccttcc acaaggctca cggcgacttc cacggccacg acggcttcga
     3901 ccgggtccgc ctgacccact ggttgaccga actgcgcttc gccgacattc agatcagcac
     3961 cgcgaccacg ctcaccaagg acgtcgacgg cgaaccacgc gagttcccga tcttcctggc
     4021 caccgcccgc aagccgggct gatcccagga tcgctcgcta ccgtcgagcc atgcgacgca
     4081 tcatccgcgc gacggccacg ctggccctcc tcaccctcgg cacggcctgc tcgggcgggt
     4141 ccggagagtc atcggggacg agtgcgccgg catccggctc cgccgcctcg gcttccggga
     4201 cagcggcttc ggcgacgacg gggggtgcga gcgccgcgcc attccagatc acggaggtcg
     4261 cggcatacga cgaaccgtgg gcgatgacgt tcctgccggg cacgccctac gcggcgatca
     4321 cccagcgggg cggccagctg atcgtccgcg acatggaggc cggtaccgat ctcgacgtcg
     4381 acaacgagcc cgaggtcgcg gccggcggcc aaggcggtct cggcgacatc atcgccgctc
     4441 cgtcctacga gcaggaccag cggatctacc tcagctgggt ggagccgggc gacaacggga
     4501 cgcgtggggc ggtcgtcggc aacgggcggc tggtcatcga cgacgtcagc gctcgcatcg
     4561 agaacctcaa tgtcatctgg cggcaggtgc cgaaggtgga gggcgagggc cacttcgggc
     4621 accggctcgc gatgagcccc gacggcacct acctgttcgt ctcctccggg gagcggcagc
     4681 agttcgaccc cgcgcaggac ccgggcacca acctcggcaa gatcctgcgg ctgaatcccg
     4741 atggctctcc cgcacaaggc aatccgctct cggaccagga atacccgtcg gacgagatct
     4801 ggtcgtgggg acaccgcaac ccgctcggtc tcgcgttcga cgccgcgggc aacctctggg
     4861 agtccgagat gggcccgaag ggcggggacg agatcaacct catccgtgcc ggcgccaact
     4921 acggctggcc caaggcgtcc aacggctcgc actacagcgg cgaggacatc ccggatcatg
     4981 cggaggggga cgggtatgaa gcgccgaagg tcttctggaa tccctccatc tcgcccggtg
     5041 ggctgatgat ctattcgggt tcggctttcc cggaatgggt cggcgatgcc ttcgtgcccg
     5101 ccctgtcggg tcagtcgctg atccgggtcg acatcgacgg gacctccgcg acggccgccg
     5161 accagtggtc cgacggcccg ctcggcgagc ggctgcggga ggtcgaacaa ggcccggacg
     5221 gcacgatctg ggtgctccag gacggcggcg gcggcaagct cctgcacctg actccgccgg
     5281 cctgaacctg aagttaggtt taccttactt cgacaggcgc ggaacaatta cctaacatgg
     5341 atgtgtgaat atttcacgcg cctcgctggc gacggccgac gccgcgcccg agtcgcgcac
     5401 gcgcgaacgc gtcctgcgtg ccgtcagcca gcagggcccg atcacgacga cggccctcgc
     5461 cgagtcgctg gagttgtccg acaccgcgat ccggcgccat gtcgacaacc tcgtgaccga
     5521 cgggctgatc gaggcgcatg acggcgcgaa cccgcgccgc gggcggggcc ggccggccaa
     5581 gtcgtatgtc gtttccgaag ccgggcacgg cgccctcacc tccgactacg accacctcgc
     5641 cggtcaggcg ctgcgttatc tcgccgagac gatggggccg caggccgtcg aggacttcgc
     5701 gcggcagcgg atcgccgact gggaggtgcg gtatgccacg cagctcgccc ccgacctcac
     5761 ccccagcgag cgtgccgagg ccctcgtcgc cgcgctgacc cgcgacgggt tcgacgccag
     5821 cctgcgccac gtcggcgggc cgggcacccc gagcgcgctg cacggcatac agctgtgcca
     5881 ggggcactgc ccggtgcgcg gcgtcgcggc gcaattcccg cagctgtgcg aggccgagac
     5941 cgaagccttc tcccgcctgc tcggcgtcca cgtgcaacgc ctggccaccc tcgcgcacgg
     6001 cgatcacgtg tgcacgacct tcatccccac ccccacgatc ccccatcaat cggaggcgtc
     6061 atgacccaga gcatcgaaga cctcaaccca ggtctgaagg gactcggtca ctacgaatac
     6121 ggctgggccg acagcgactc ggccggtgcg tccgcgcgcc gcggcctcaa caccgatgtc
     6181 gtggaagaca tttcgcgccg caagagcgag ccggagtgga tgcgtgagct gcgcctgaag
     6241 tcgctgcgcc tcttcgacaa gaagccgatg ccgacctggg gcagcgacct gacgggcatc
     6301 gacttccaga acatcaaata ctttgtgaag tccaccgaaa agcaggccac ctcctgggaa
     6361 gaccttccgc aggacatcaa gaatacgtac gaccggcttg gaattccgga agcggaaaag
     6421 caaagattgg tcgccggagt ggccgcgcaa tacgaaagtg aagtggttta ccaccagatc
     6481 cgcgaggatt tggaggaaaa gggtgtcatt ttcgtcgaca ccgacacggg attgcgcgag
     6541 cacgaagacc tcttccgcga gtacttcgga tcggtaattc ccccggggga caacaagttc
     6601 gcctcgctca acacggccgt ctggtcgggc gggtcgttca tctatgtccc gccgggtgtg
     6661 cacgtcgaca tcccgctgca ggcctatttc cggatcaaca ccgagaacat gggccagttc
     6721 gagcggacgc tgatcatcgc c
//
LOCUS       DAOPJG010000179         6719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_12076_length_6719_cov_8.063625, whole genome shotgun sequence.
ACCESSION   DAOPJG010000179 DAOPJG010000000
VERSION     DAOPJG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12076_length_6719_cov_8.063625"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PKU75_13725"
     CDS             complement(<1..934)
                     /locus_tag="PKU75_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPF81984.1"
                     /translation="MPAVRPADAVAVAEDYLHERGPTLESTHPLALGPVTAPPHTVDG
                     FVKEFLRELNYGQGVPLSRSSLNDQYMALARTVRGYLTARWLETATRRRQSPHKMVGY
                     LSAEYLLGRQLDNALLATDLSAIAEEAMKVCGLELDALRQAEVEPGLGNGGLGRLAAC
                     FIDSLATMDAPCVGYGIRYEYGIFKQTFVDGRQVEVPDNWLSMGGPWEVAHPEMEVPV
                     HFGGHVERATGDDGRERCTWVPDWSVLGIRYNVMVPGYQNGVVNTLRLWRAIATRAFD
                     LDIFNAGDYASAVRSQTVAENISKVLYPEDSTPQG"
     gene            complement(1053..1997)
                     /locus_tag="PKU75_13730"
     CDS             complement(1053..1997)
                     /locus_tag="PKU75_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HPF81985.1"
                     /translation="MAGGLVALLDDIAALAKLAAASIDDVGLAAGRATSKAAGVVIDD
                     TAVTPRYVDGFTPDRELPVIKKIAIGSLRNKLLFITPAALLLSQFAPWALTPLLMIGG
                     SYLCYEGAEKVWEKFGGGHHAEDETPVVLESAEQEKLMTAGAIRTDFILSSEIMVISL
                     NEVAHEPFLMRTAILIVVAIAMTLLVYGVVALIVKMDDVGLHLAKAGTGAKATIGRGM
                     VAGMPKVLAALSTIGVVAMLWVGGHIVLKGTDDLGWHTLYGWVHHLEEVAHDLGGAVL
                     GWLANTAASAVFGLLWGAVIVAVMTLVHKARSGEPAHH"
     gene            2203..3492
                     /locus_tag="PKU75_13735"
     CDS             2203..3492
                     /locus_tag="PKU75_13735"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF81986.1"
                     /translation="MSSPRSDLLRDRPFALLLTARTLSMLALAFAPVALAFGVLDLPG
                     ASATTLSTVLAAESIALVLFTLVGGVVADRYPREQVLRAAEWANAVLHIVIGVLLLSG
                     HAPVWGLVTLTFLAGTAGALVWPAMTGIVPQVVPAHSLQDGNALIGIGGNTARVAGLV
                     AGGIVVVAIGGGWALIAAGMIFAVAGLCVGRLRLTRPPQDDVPGPGMLRELRVGWSEF
                     RSRTWLWVCVLQFSAFVMVWQAAHLVLGPVVAKTALGGPGAWSTILTVESCGLILGGL
                     VAMRWRPAYPVRAVVLLSFLGAPPYLLLGLHAPLVAIAVASFFLGLAFEMLSIFWQTT
                     MQREIPAESLSRVSSYDALGSLALGPLGIILAGPAADAVGAQRVLLVCAAVMLVTSVS
                     SLSVPSIRSLRARPDVQSLPPAPDSPADLGPAITLPQ"
     gene            complement(3508..4272)
                     /locus_tag="PKU75_13740"
     CDS             complement(3508..4272)
                     /locus_tag="PKU75_13740"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF81987.1"
                     /translation="MGNTGLDAPTPEHLDRIVASYAGSRPGYPAAVFDQLRKAHVVGP
                     GVRVLEIGAGTGEATTDLVATGADVTAVEPRPVLAKTLAERVPQARVVVQRLEDLDLA
                     PGSFDAAIGATSLHWLSLDTALPMLHDALRAQGRLAVLRTTFGDASVKTPFRRRVAEI
                     VKSRVEATADKRGLRPTMRELTESGLFLPVDSWEWPWSIELTAGQIERLFTTFSDWSP
                     AEVAQARAAADDLGGKIVEHYRTWLHVLEKAPGPRA"
     gene            complement(4333..5208)
                     /locus_tag="PKU75_13745"
     CDS             complement(4333..5208)
                     /locus_tag="PKU75_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81988.1"
                     /translation="MAYDAQRQIIRTLARAAREFDRARDALMGEEQTETTVPDSASSA
                     VPPEAAQPARGGVLGAATQVARAGAGAAKAAGRAAAGVHPRHPDWQTAILTDRIAWWV
                     QRFGTAAAALTAVPGLLGKVGRLTGVGDVIGAAAQVLIVNAVAREMGIEDIPRRVAAA
                     ADIVLDRQLSMAEIELALAEEATPATGQNSAERADTGQDDTNQDDTNQDDIRPDDTDQ
                     PGIVRRLGGSAALVGRVARDLWRLNSDMDKRPGAGRVVGFARNLPAVGAVSSFAYERR
                     GIARAAERARSRFGG"
     gene            complement(5223..5462)
                     /locus_tag="PKU75_13750"
     CDS             complement(5223..5462)
                     /locus_tag="PKU75_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81989.1"
                     /translation="MLLRLLVIDGESARQLRSAGLNLDSARTAMAAMAPDGNCRLTLS
                     LRVDREGRHRLLDRADDQTTAIRLNLIAQALAQAS"
     gene            complement(5573..5791)
                     /locus_tag="PKU75_13755"
     CDS             complement(5573..5791)
                     /locus_tag="PKU75_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81990.1"
                     /translation="MERQIEAVAADIADPMLGLRAVVALRRLADQLELRQVQAALDAG
                     HAWPEIAGALGVTRQAVHKKYHRPVTTR"
     gene            5933..>6719
                     /locus_tag="PKU75_13760"
     CDS             5933..>6719
                     /locus_tag="PKU75_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPF81991.1"
                     /translation="MAKATAATTASGADQARAEGQGSARHTAGTPHTADSHDTIRVVG
                     ARENNLKGVDVELPKRRLTVFTGVSGSGKSSLVFATIAAESQRMINETYSTFVQGFMP
                     TLARPDVDLLEGLTTAIIVDQERMGANIRSTVGTVTDVNAMLRILFSRLGQPHIGGSK
                     AFSFNVASASGAGAVTTTKGGMEVKERREFSIQGGMCARCEGVGKVSDFDLTALYDDE
                     KSLREGALTIPGYSMDGWYGRVYMGSGFFDPDKPIKKFTERELA"
BASE COUNT          975 a         2234 c         2409 g         1101 t
ORIGIN      
        1 tgccctgcgg cgtggagtcc tcggggtaga gcaccttgga gatgttctcg gcgacggtct
       61 gcgaacgcac cgccgaggcg tagtcaccgg cgttgaagat gtcgagatcg aaggcgcggg
      121 tcgcgatggc ccgccacagt cgcaaggtgt tgacgacgcc gttctggtag ccggggacca
      181 tcacgttgta acggattccc agcacgctcc agtcgggcac ccaagtgcag cgttcgcgtc
      241 cgtcgtcgcc ggtggcgcgt tcgacgtgcc cgccgaagtg gacagggacc tccatctcgg
      301 ggtgggcgac ctcccacggg ccacccatgg acagccagtt gtcggggacc tcgacctggc
      361 ggccgtcgac gaaggtctgc ttgaagatgc cgtactcgta gcggatgccg tagccgacgc
      421 acggcgcatc catggtcgcg agcgagtcga tgaagcaggc ggcgagccgg ccgagaccgc
      481 cgttgccgag gccgggctcg acctcggcct gccgcagcgc gtccaactcc agaccgcata
      541 ccttcatcgc ctcctcggcg atggcgctca ggtcggtggc caggagggcg ttgtcgagct
      601 ggcgccccag gaggtattcg gccgagaggt agccgaccat cttgtgcggg ctctgccgac
      661 ggcgggtcgc cgtctccagc caccgggccg tcaggtagcc gcggaccgtg cgcgcgagcg
      721 ccatgtactg gtcgttgagc gacgagcgcg acaggggcac gccctggccg tagttgagtt
      781 cgcgcaggaa ctccttgacg aagccgtcga ccgtatgcgg tggggccgtg accggaccca
      841 gcgccagcgg gtgcgtggac tcgagcgtcg gaccccgctc gtggaggtag tcctcggcta
      901 cggcgacggc gtccgcaggg cggacggccg gcatggtgtc agacacgttg ggtcactttc
      961 actgggctcg tgtaagcctt ttcagggcac atgtcacgag cataaggccc cggcgacgct
     1021 gtcgggctgg tctcggaagt ctccgagaag catcagtggt gcgcaggctc gccggagcga
     1081 gccttgtgga ccagggtcat gacggcgacg atgacggctc cccacaggag cccgaagacc
     1141 gccgacgctg cggtgttggc gagccaaccg aggaccgcgc cgccgaggtc gtgggccacc
     1201 tcctccaggt ggtggaccca gccgtagagc gtgtgccacc cgaggtcgtc ggtgcccttg
     1261 aggacgatgt gcccgccgac ccacagcatg gccacgacac cgatcgtcga cagggccgcc
     1321 agcaccttcg gcataccggc gaccattccc cgcccgatgg tggccttggc ccccgtgccc
     1381 gccttggcca ggtgcaggcc gacgtcgtcc atcttgacga tcagcgccac cactccatac
     1441 accagcaggg tcatcgcgat cgcgacaacg atgaggatcg cggtgcgcat gaggaagggc
     1501 tcgtgggcga cctcgttgag cgagatgacc atgatctcgg aggagaggat gaagtcggtc
     1561 cggatggctc cggccgtcat cagtttttcc tgctccgcac tctcgagcac gaccggagtc
     1621 tcgtcctcgg cgtggtggcc gccgccgaac ttctcccaca ccttctccgc gccctcgtag
     1681 cacaggtagc tgccgccgat catgagcagc ggggtcagcg cccacggggc gaactggctg
     1741 agcagcagcg cggccggcgt gatgaagagg agtttgttgc gcagcgagcc gatcgcgatc
     1801 ttcttgatga ccggcagttc gcgatcggga gtgaatccgt cgacatagcg cggcgtcacg
     1861 gcggtgtcgt cgatgacgac cccggcggcc ttcgacgtcg cccggccggc ggccaggccg
     1921 acgtcgtcaa tggaggccgc ggccaacttg gccagcgcgg cgatgtcgtc cagcagggcg
     1981 acgagtccgc cggccatgag caactcctga ggtcgggggt tcggacggga gcactcaagg
     2041 ataacggctg gtgccgccca gatctgaatc agcgcggctg caccccggca tgcatccaat
     2101 cgcgtcacga agcagccgtc gtccgggagc ggggcggcag ggagcggggc ggcaggggga
     2161 atcgagtggc cggcgcgtgc ggcctggccc tacggtggag cggtgtcctc cccacgctcc
     2221 gatctgctgc gcgaccgccc gttcgcgctg ctgctgacgg cgcggacgct ttcgatgctg
     2281 gcgctcgcct tcgcgcccgt ggcgctggcg ttcggggtgc tcgacctgcc gggggcgagc
     2341 gcgacgaccc tctcgacggt cctggccgcg gagtcgatcg cgctggtgct cttcacgctc
     2401 gtcggtggcg tggtcgccga ccgttatccg cgcgagcagg tgctccgcgc ggccgaatgg
     2461 gccaatgcag tgctgcatat cgtcatcggg gtgctgctgt tgagcggaca tgcacccgta
     2521 tggggtctgg tcaccttgac cttcctcgcg ggcaccgcgg gtgccctggt ctggccggcg
     2581 atgacgggga tcgtgccgca ggtcgtgccc gcgcactccc tccaggacgg caacgccctg
     2641 atcgggatcg gcggcaacac cgcccgagtg gccggcctcg tcgccggcgg catcgtcgtc
     2701 gtcgccatcg gtggcggctg ggcgctgatc gccgctggaa tgatcttcgc cgtggccggc
     2761 ttgtgtgtcg ggcggctccg gctcacgcgg cccccgcagg acgacgtgcc cggtcccggg
     2821 atgcttcgtg agctgcgggt cggctggagt gagttccggt cgcggacgtg gctgtgggtg
     2881 tgcgtgctgc agttctccgc tttcgtcatg gtgtggcagg cggcgcatct ggtgctcggg
     2941 ccggtcgtcg cgaagaccgc actcgggggg ccgggagcgt ggtcgacgat cctgaccgtc
     3001 gagtcctgcg ggctgatcct cgggggactc gttgcgatgc ggtggcggcc ggcatacccg
     3061 gtccgggcgg tggtgctgct gagcttcctg ggggcgccgc cctatctgtt gctcggtctg
     3121 cacgcacccc tcgtggcgat cgcggtcgca tcgttcttcc tcgggctcgc cttcgagatg
     3181 ctctcgatct tctggcagac gacgatgcag cgcgagatcc cggccgagtc gctctcgcgc
     3241 gtcagttcgt atgacgcgct gggctccctg gcgcttgggc cgctcggcat catcctcgcc
     3301 gggccggcgg cggatgccgt tggggcacaa cgggttctgc tcgtgtgtgc ggctgtgatg
     3361 ctggtgacca gcgttagctc cctgtccgtc ccgagcattc gcagtctgcg ggcgcgcccg
     3421 gacgtgcagt cgttgccgcc ggcgccggat tcacccgccg acctcggccc cgcaatcacc
     3481 ctgccgcagt agctcggcgc ggggtagcta ggcgcgtggt ccgggcgcct tctccaggac
     3541 gtgcaaccac gtccggtagt gctcgacgat cttgccgccg aggtcgtcgg ccgcggcgcg
     3601 cgcctgggcg acctccgccg gcgaccagtc gctgaaggtc gtgaagagcc gctcgatctg
     3661 gccggcggtc aattcgatcg accacggcca ctcccacgaa tcgacgggga ggaacagccc
     3721 gctctcggtc aactcccgca tcgtcgggcg tagaccgcgt ttgtcggcag tggcctcgac
     3781 ccgggacttg acgatctctg cgacgcggcg gcggaacggg gtcttgaccg acgcgtcccc
     3841 gaaggtggtc cgcagcaccg cgagccgacc ctgggcccgc agcgcgtcgt gcagcatcgg
     3901 cagggcagtg tcgaggctga gccagtgcag tgaagtggca ccgatggcgg catcgaagct
     3961 gcccggcgcg agatccagat cttccagacg ttgcacgacc acccgggcct gcgggacgcg
     4021 ctccgcgagg gtcttcgcga gcaccggccg tggttcgacc gctgtcacgt cggcgccggt
     4081 ggcgaccaga tccgtcgttg cctcacccgt gccggccccg atctccagca cccggacacc
     4141 cggtccgacc acgtgtgcct tgcgcaactg gtcgaagacg gctgccgggt atcccgggcg
     4201 agaaccggca tacgacgcga cgatgcggtc cagatgttcg ggcgtcgggg cgtccaagcc
     4261 tgtgttcccc atggacggca ggctaacccc tcaagatgaa ccgtcgacgt gaacggtcga
     4321 ctccacctgc ggtcatcccc cgaagcggct gcgggcgcgc tcggcggcac gcgcgatgcc
     4381 gcgtcgttcg taggcgaagg aactcacggc gccgaccgcc ggcaggttgc gggcgaaccc
     4441 gacgacgcgg ccggctccgg ggcgcttgtc catatcgctg ttgaggcgcc acaggtcacg
     4501 cgccacccgc ccgacgaggg cggcactgcc gccgaggcgc cgcacgatcc cgggctggtc
     4561 agtgtcgtcc ggtctgatgt cgtcctgatt ggtgtcgtcc tgattggtgt cgtcctgacc
     4621 cgtgtcggcg cgctcggcgg agttctggcc ggtggcggga gtggcctcct cggcgagcgc
     4681 gagttcgatc tcggccatcg acaactgccg gtcgagcacg atgtcggctg ccgcggcgac
     4741 tcgccgggga atgtcctcga tgcccatctc gcgtgcgacg gcgttgacta tcaggacctg
     4801 ggcggcggcg ccgatgacgt cgccgacgcc ggtgagccgg ccgactttgc cgagcaggcc
     4861 ggggaccgcg gtcagggccg cagcggcggt gccgaagcgt tgcacccacc acgcgatgcg
     4921 gtcggtgagg atggccgtct gccagtccgg gtgccgcgga tgcacgcccg ccgccgctcg
     4981 gccggccgcc ttcgctgccc cggccccggc acgcgcaacc tgagtcgccg caccgaggac
     5041 gccgccacga gcgggctggg ctgcttcggg tgggacggcg ctggaggcag agtcaggaac
     5101 ggtcgtctcg gtttgctcct cgcccatcag ggcatcgcgt gccctgtcga actcccgggc
     5161 ggctcgagcc agggtacgga tgatctggcg ctgggcgtca tatgccatgt ctcaagaatg
     5221 actcacgacg cttgggccag cgcttgggcg atcaggttca gccggatcgc ggtcgtctgg
     5281 tcatcggcac ggtccaggag gcgatgccgg ccttcccgat cgactcgcaa cgagagcgtc
     5341 aggcgacagt tgccgtcggg cgccatggcg gccattgcgg tgcgggccga gtccaggttc
     5401 agcccggccg agcgcaactg cctggcgctc tcaccgtcga tgaccaggag cctcagcagc
     5461 acgtggtcgc tgtcgactgc gtgtgcccgg cgcgcagcgc ctcgtcgttg gcagcgatca
     5521 ggagccgcca cacccgctcc aggttcgtca cggtttgctt caacatcttc cctcacctcg
     5581 tggtcacggg tcggtggtac ttcttgtgga cggcctgccg ggtcaccccg agggcccccg
     5641 cgatctccgg ccacgcatgt cccgcgtcca gggcggcttg gacctggcgc agttccagtt
     5701 ggtcggccag ccggcgcagg gcaacgacgg cccgcaggcc cagcatcggg tccgcgatat
     5761 cggccgctac ggcctcgatc tgtcgctcca tacctgtcaa cctaggttgc tcgagcaatt
     5821 gtcaacctga gttgacgggc agcgatggga aatcgcgact ccggctcggc gcggcactag
     5881 ggtgagcggg ccggaggtgc cggctcccat tcctgaaggc gaaggcgaga agatggcgaa
     5941 ggccacggcc gcgacgacgg cgagcggggc ggatcaggcg cgtgcggagg gacaggggag
     6001 cgcgcggcat acggcgggca caccgcatac ggcggactcg cacgacacca tcagggtcgt
     6061 cggggcgcgg gagaacaatc tcaagggggt cgacgtcgaa ctgcccaaac ggcggctgac
     6121 ggtcttcacc ggagtgtccg ggtcaggcaa gagttcgctc gtcttcgcga ccatcgcggc
     6181 cgagagccag cggatgatca acgagaccta ttcgaccttc gtccagggct tcatgccgac
     6241 cctggcgcgg ccggatgtcg atctgctgga agggctgacg acggcgatca tcgtcgatca
     6301 ggagcggatg ggcgccaaca tccggtccac ggtcggcacc gtcaccgacg tcaacgccat
     6361 gctgcggatc ctcttcagcc ggctgggcca accacacatc gggggctcga aagcgttctc
     6421 cttcaacgtc gcctcggcct cgggggcggg ggcggtgacg acgaccaaag gcggcatgga
     6481 ggtcaaggag cggcgcgagt tcagcatcca gggcggcatg tgcgcgcggt gcgagggggt
     6541 cggcaaggtc tccgacttcg acctgaccgc cctctatgac gacgagaaat cgctgcgcga
     6601 gggtgcgctg acgatccccg ggtactcgat ggacgggtgg tacggccggg tctacatggg
     6661 cagtggcttc ttcgatccgg acaagccgat caagaagttc accgagaggg aactcgcgg
//
LOCUS       DAOPJG010000180         6707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_12113_length_6707_cov_8.459110, whole genome shotgun sequence.
ACCESSION   DAOPJG010000180 DAOPJG010000000
VERSION     DAOPJG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12113_length_6707_cov_8.459110"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..673
                     /locus_tag="PKU75_13765"
     CDS             104..673
                     /locus_tag="PKU75_13765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81992.1"
                     /translation="MGPLADASVREARATDAPAVGQVQAQVFIDTYADKLLPEVAAAF
                     DPQAFARGWRDALTDPPPGAHRLLVALAGAQVVGAAAYGPSQDPDTGQATGELTLLAV
                     HPLARRQGHGSRLLNACVDQLRDLGADTVTAWLLADAEETRAFLLGAGLEPDGAYRDR
                     VVSPDGATLREVRVRATLGQDTDDDAQGG"
     gene            677..1408
                     /locus_tag="PKU75_13770"
     CDS             677..1408
                     /locus_tag="PKU75_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HPF81993.1"
                     /translation="MTSDQAVCDARRKEVVRQGISVGVATGLYGVSFGALAVAAGLSV
                     LQTQALSLLMFTGGSQFALVGILAAGGSGASAIATSSLLGIRNGLYALQTSQFLQARG
                     LRRLLAAHITIDESTAVGIAQKERPLQRVGFWVTGLAVLAGWNLMTLVGALVGNALGE
                     PETWGLDAAAAGAFCALLWPRLQTGDGRATAVLAAFIALITIPLVPAGIPVILAALAA
                     VVVGLMAHGRHERGEDGLPGSDPLP"
     gene            1405..1719
                     /locus_tag="PKU75_13775"
     CDS             1405..1719
                     /locus_tag="PKU75_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HPF81994.1"
                     /translation="MNTWVTVLAACALAFLLKLSGYFVPARLMEGPRTSRISALLPAA
                     LLAGLIVTQTVAGSGGRIVLDARLAAVAVAAILLWRKANFLVVVGAAAATAALLRLSG
                     WT"
     gene            complement(1716..2495)
                     /locus_tag="PKU75_13780"
     CDS             complement(1716..2495)
                     /locus_tag="PKU75_13780"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPF81995.1"
                     /translation="MEQDDTARVTVPKALTEIAARSPRVVVLTGAGMSAESGLATFRD
                     PHTVLWERFSPQALASASAWEDDPELVWAWYLWRFERIEHVAPNAGHVAIARWGRGAD
                     VSVVTQNVDDLHERAGSADVVHVHGAIASFRCSVCEAPYAEPIDVPDEPVERLPPPVC
                     RVCGGLVRPGVVWFGEVLPVDAWTRAVALVSEADLVVVVGTSGIVYPAAGLPAIARSE
                     GAVVIEINPDRTDVSDMVHHTWHETAARALPALVGSFLPAG"
     gene            complement(2549..3301)
                     /gene="dapB"
                     /locus_tag="PKU75_13785"
     CDS             complement(2549..3301)
                     /gene="dapB"
                     /locus_tag="PKU75_13785"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPF81996.1"
                     /translation="MSDKSIKVAVIGAAGRMGSTVCDAVDLAPDTELVGRFDVGDDLG
                     GLGGADVVVEFSVPDASLTNVTHCVEQGVHVVVGTTGWTEDKLDKLRKTVAAHKDVGV
                     LIAPNFAIGAILMMRFAEQAARFYESVEVVELHHPNKVDAPSGTAARTADLIGEARKA
                     AGLGPVPDATTQDPEGARGGRFNGIPVHSVRLRGLVAHQEVLFGAEGELLTIRHDSLD
                     RASFMPGVLTGVRQVAGHPGVTVGLENYLGLS"
     gene            3436..4323
                     /locus_tag="PKU75_13790"
     CDS             3436..4323
                     /locus_tag="PKU75_13790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF81997.1"
                     /translation="MKRVVDLEWYDDPRAFESDAQGFLASDALRATVISSMLVRLLAG
                     DQVVAKTAPTPYWFAVQRDASGAPDGLAMRSGDWPPYLLDVRPDAARRLAGEVLARGE
                     RPGGANGTLASAQAFCERLIEEIGGAVHVERATRLFQVTHLVPPTGVPGEGRLARADE
                     VDFCTDWFLAFHDEVGEAPTASRKEQCAEYRAIVADRTAQDCLWLWEDDGRVVSLAGA
                     RPAAFGFARIGPVYTPPENRGHGFAAAVTCLAALTIQGRDATPCLFTDLANPTSNGVY
                     QRIGFEPVEDTVNLRIGAP"
     gene            4320..>5087
                     /locus_tag="PKU75_13795"
     CDS             4320..>5087
                     /locus_tag="PKU75_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF81998.1"
                     /translation="MTTHDWPVIWAGPEWRAEVSAWIGDVLHAKGCRITGPIEQPRIQ
                     FWSTQLVVPSDRGRVWLKQNNAGQSFEAALVARLAELAPGRVVEPIAVDHERGLLLSP
                     DQGPTLRERDGGTAADWTGVLAEFGALQRQLSPYAAEIAATGRPGLRARDTPAYVEQR
                     IDELARLGGGDPRGLSREDAAAATMALPELVDACTELDASGIPDSLQHNDLHDDSAFP
                     SVAGAAEVRRFRRRRVEPPVRRAAHRERVDGASARHDA"
     assembly_gap    5088..5187
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5188..6362)
                     /locus_tag="PKU75_13800"
     CDS             complement(<5188..6362)
                     /locus_tag="PKU75_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPF81999.1"
                     /translation="MSVDVSERHTTSVAEAPEDFFGEEVLSDRDRYYAAWRESAAVVH
                     LTRNDLWALTRYDAIQFALATPAIFSSTKAVFNDMMNDALRGSALASDPPEHAPLRAV
                     LAKYLSPRVVRSRQSDMETYADELVSSAAAQGSFDAIRLAQAYHQRIIGEMIGMRREI
                     QARVVPWGDSALNVFGPFNQRTFKHLPVAGDLVRWAMQVTPDQLGEGSVGRAVFEAAD
                     RGEIPHEACPGLIHQLVAAGIDTAIASLGNLIAHLGDNPAQYAILRAEPGLAGAAFTE
                     SLRLESPIHAMGRRVTEDVEVDGIPIPEGAQVALLFAAGNRDPRHYDDPDTFDIRRNP
                     VDHLSFGHGVHTCAGMHLVRHEAEALLAALVRHVPSYSIGERRPKLSNLVSSWGTLTV
                     "
BASE COUNT          938 a         2400 c         2275 g          994 t
ORIGIN      
        1 cgaccccgag ccccaggatc acgagcgcca ggaccccgag ccccaggatc accagcccca
       61 ggatcaccag ccccaggatc accagcccca ggatcaccgg acattgggac ctctcgcgga
      121 tgcctccgtc cgcgaggccc gggcgaccga tgccccggcc gtgggtcagg tccaggcgca
      181 ggtcttcatc gacacctacg ccgacaagct gctgcccgag gtcgccgcgg cgttcgatcc
      241 ccaggcgttc gcgagaggct ggcgcgatgc cctgaccgac ccgccgccgg gtgcccaccg
      301 gctgcttgtc gcgctggccg gcgcccaagt ggtcggggcc gcggcatacg gcccgagcca
      361 ggatcccgac accggccagg ccaccggcga gctgaccctg ctcgccgtgc atcccctggc
      421 ccggcgccag ggccacggct cgcgactgct caacgcgtgc gtcgaccagt tgcgcgacct
      481 gggcgccgac accgtcaccg cgtggctcct ggccgacgcc gaggagaccc gtgccttcct
      541 gctcggcgcc gggctcgaac cggacggcgc ctaccgtgac cgcgtcgtct cgcccgatgg
      601 cgccaccctg cgcgaagtcc gggtccgtgc gaccctcggc caggacaccg acgacgacgc
      661 gcaggggggg tgacgggtga ccagtgacca agctgtctgc gatgcacggc gcaaagaggt
      721 tgttcgtcaa gggatctcgg tcggcgtcgc tacgggcctc tacggcgtca gcttcggggc
      781 gcttgcggtc gccgcgggtc tgagcgtcct gcagacgcag gcgctctccc tcttgatgtt
      841 caccggcggt tcgcagttcg ccctcgtggg catcctggcc gcgggcggtt ccggcgcctc
      901 cgccatcgcc acgtcctccc tgctcggcat ccgcaacggc ctgtatgccc tgcagacctc
      961 acagttcctg caggcccgcg gcctgagacg actgctcgct gcacacatca cgatcgacga
     1021 atcgaccgct gtcggcatcg cgcagaagga acgtccgctc cagcgggtcg gcttctgggt
     1081 cacggggctc gccgtgctcg ccggctggaa cctcatgacc ctcgtcgggg cgctggtcgg
     1141 caatgcgctc ggcgaacccg agacctgggg gctcgacgcg gctgccgccg gcgccttctg
     1201 cgcgctgctg tggccgcgcc tgcagaccgg tgacgggcgg gccacggcgg tgctcgccgc
     1261 gttcatcgcc ctgatcacca ttccccttgt gccagctggg atcccggtca tcctggcggc
     1321 cctcgccgct gtcgtcgtcg gtctcatggc acacggccgg catgagcgcg gcgaggacgg
     1381 gctccccgga tcggacccgc tgccatgaac acctgggtca cggtcctcgc cgcctgtgcg
     1441 cttgccttcc ttctcaaact ctcgggctat ttcgtgcccg cgcggttgat ggaggggccg
     1501 cgaacatcac gcatcagcgc cctgctgccg gctgcccttc ttgccgggct gatcgtcacc
     1561 cagaccgtgg ccggatccgg cggccgaatc gtgctcgacg cccgcctcgc ggccgtcgcc
     1621 gtcgctgcga tcctgttgtg gcgcaaggcg aacttcctcg tcgtcgttgg agcggccgcc
     1681 gccacagccg ccctgctgcg cctctccggc tggacctaac ccgcgggcaa gaacgacccg
     1741 accagcgccg gtagcgcccg ggcagccgtc tcgtgccacg tgtggtgcac catgtcggag
     1801 acatcggtcc ggtcgggatt gatctcgatg acgacggccc cctcggagcg agcgatagcg
     1861 ggcaggcccg ccgcgggata gacgatgccg ctggtgccga ccacgaccac gaggtcggcc
     1921 tcggacacga gggcaacagc gcgggtccac gcgtcgacgg gcaacacctc gccgaaccac
     1981 acgacgccgg gccggaccag accgccgcag acgcggcata ccggcggggg caggcgctcc
     2041 accggctcgt ccgggacgtc gatcggctcg gcatacggtg cctcgcagac actgcagcgg
     2101 aacgaggcga tcgccccgtg gacgtggacg acgtcggcgc tcccggcgcg ctcatggagg
     2161 tcgtcgacgt tctgggtcac gacggacaca tcggcgccgc gaccccaccg cgcgatcgcg
     2221 acgtgcccgg cgttcggggc cacgtgttcg atgcgctcga aacgccagag ataccacgcc
     2281 cagacgagct ccgggtcatc ctcccacgcg ctggccgacg ccagcgcctg tggcgaaaac
     2341 cgttcccaca gaacggtatg cggatcgcgg aaggtggcca gaccgctttc ggctgacatg
     2401 cccgcgccgg tgaggacgac cacccggggc gaacgggccg cgatctcggt cagggccttg
     2461 ggcacggtga cgcgagccgt gtcgtcctgc tccacgatcc tctcccttca cctgcggatc
     2521 gaccgggtcg gcgcacttca gcgtgccgtc aactcagccc gagatagttc tccagcccga
     2581 ccgtgacgcc agggtggccg gccacctgcc ggaccccggt cagcacgccc ggcatgaacg
     2641 aggcccggtc gagactgtcg tggcggatcg tgagcagttc gccctcggcg ccgaagagca
     2701 cctcctggtg cgcgacgaga ccacgcaggc gcaccgaatg caccggtatg ccgttgaacc
     2761 gccccccgcg ggccccctcc gggtcctggg tggtcgcgtc cgggaccggg cccagtccgg
     2821 cggccttgcg cgcctcgccg atcaggtcgg cagtgcgagc cgccgtgccc gacggggcat
     2881 cgaccttgtt ggggtggtgg agttcgacga cctcgaccga ctcgtagaag cgggccgcct
     2941 gctcggcgaa gcgcatcatc aagatggcac cgatggcgaa gttcggcgcg atcaggacac
     3001 cgacatcctt gtgcgcggca acggttttgc gcagcttgtc gagtttgtcc tcggtccagc
     3061 ccgtcgtgcc gacgacgacg tggacaccct gctcgacaca gtgggtgaca ttcgtcaggg
     3121 acgcgtccgg gaccgagaac tcgaccacga cgtccgctcc gccgagaccc ccgaggtcgt
     3181 cgcccacgtc gaagcgcccg accagctcgg tgtcgggggc caggtcgacg gcgtcgcaca
     3241 cggtcgatcc catgcgtccg gcggcgccga tgacggcgac cttgatgctc ttgtcactca
     3301 ttcccacacc ctagatcgcc gggagcctcg atcggggtcg ccacctcgcg cacagagtcg
     3361 tgggtcctgg gccggtcgtg ccctggccac ggtccggccg aaaaccccgg cgggcagacc
     3421 atctcgtgtc ctaccgtgaa acgcgtggtg gacctggagt ggtatgacga tccgcgagct
     3481 ttcgagtccg acgcgcaggg attcctggcg tcggatgcgt tgcgcgccac cgtgatctcg
     3541 tccatgctgg ttcgcctgct cgcgggcgat caggtcgtcg ccaaaacggc gcccacgccg
     3601 tattggttcg ccgtccagcg cgacgcctcc ggagcgcccg acggtctcgc catgcgcagc
     3661 ggcgactggc cgccctacct tctcgatgtc cgccccgacg ccgccaggcg ccttgcgggc
     3721 gaggtgctcg cccgcggcga gcgaccgggc ggagccaatg gcacgcttgc ttcggctcag
     3781 gccttctgcg agcgtctgat cgaggagatc ggcggggcgg tccacgtcga gcgggcgacc
     3841 cgcctcttcc aggtcaccca cctcgtgcca ccgacgggcg tgcccggtga gggccgcctc
     3901 gctcgggcgg acgaggtgga cttctgcacc gactggttcc tggccttcca tgacgaggtc
     3961 ggcgaggcgc cgactgcttc tcgcaaggag cagtgcgcgg agtatcgcgc gatcgtcgct
     4021 gaccggaccg cccaggactg cctgtggctg tgggaagacg acggacgcgt cgtctcgctg
     4081 gccggggccc gcccggccgc tttcggattc gcgcgtatcg gcccggtcta cacaccgccg
     4141 gagaaccgcg gtcacgggtt cgccgcggcc gtcacctgcc ttgcggcgct gacgattcag
     4201 ggacgcgacg ccacaccgtg cctgttcacc gacctcgcca acccgaccag caacggggtc
     4261 taccaacgga tcgggttcga gccggtcgag gacaccgtca acctccggat cggagcgcca
     4321 tgaccacgca cgattggccg gtcatctggg ccgggccaga gtggcgggcc gaggtgtccg
     4381 cgtggatcgg ggatgtgctg cacgccaaag gatgccggat caccgggccg atcgaacagc
     4441 cgcgcatcca gttctggtcg acgcaactcg tcgtccccag cgaccgcgga cgcgtgtggc
     4501 tcaagcagaa caacgccggg caatccttcg aagcagccct cgtcgcccga ctcgccgagc
     4561 tcgcgcccgg ccgggtggtc gaaccgatcg ccgtggacca tgagcgcggc ttgctgctct
     4621 cccccgacca gggcccgacc cttcgcgaac gcgatggggg caccgcagcg gactggaccg
     4681 gggtgctcgc ggaattcggt gcgctgcaac gacaactctc cccctacgcg gcggagatcg
     4741 ccgcgacggg tcggccggga ctgcgcgccc gcgacacccc ggcatacgtc gagcaacgta
     4801 tcgacgaact ggcccggctc ggcggcggcg atccgcgcgg actcagccgc gaggatgcgg
     4861 cagcggccac catggccttg cccgaactcg tggacgcctg cacggaactc gacgcgagcg
     4921 gcataccgga ctcgttgcag cacaacgatc tgcacgacga cagcgccttc cccagcgtgg
     4981 ccggggccgc tgaagttcgt cgatttcggc gacgccgtgt ggagccaccg gttcgccgtg
     5041 ctgcacatcg cgagcgcgtc gatggcgcga gcgctcggca cgacgccnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnacc gtcagcgtcc cccaactgct gaccaggttg
     5221 ctcagcttgg gccggcgctc gccgatggag tagctcggca cgtgccgcac cagggcggcg
     5281 agcagggctt cggcctcgtg ccgcacgagg tgcatgccgg cgcaggtgtg cactccgtgg
     5341 ccgaaggaga gatggtcgac cgggttgcgg cggatgtcga aggtgtcggg gtcgtcgtag
     5401 tgtcgcgggt cccggttgcc cgccgcgaag agcagcgcca cctgcgcccc ctccgggatc
     5461 ggtatgccgt cgacctccac gtcctcggtg acccggcggc ccatggcgtg gatcggtgac
     5521 tccaaccgga gggactcggt gaaggccgcc cccgccaggc cgggctcggc gcgcaggatc
     5581 gcgtattggg cggggttgtc gcccaggtgg gcgatgaggt tgcccagcga ggcgatcgcc
     5641 gtgtcgatcc ccgccgcgac gagttgatgg atcaggccgg ggcacgcctc gtgcgggatc
     5701 tcgccccggt cggcggcctc gaacaccgcc cggccgacgc tgccctcgcc gagctggtcc
     5761 ggcgtgacct gcatggccca cctgaccagg tcgcccgcaa ccggcaggtg cttgaacgtg
     5821 cgctggttga acggcccgaa gacgttgagc gcggaatccc cccacggcac gacgcgcgcc
     5881 tggatctcgc ggcgcatccc gatcatctcg ccgatgatcc gctggtggta tgcctgcgcc
     5941 agccggatcg cgtcgaagct gccctgtgcc gccgcgctgc tgaccagctc atcggcatac
     6001 gtctccatgt cgctctggcg cgagcgcacc acccgcggcg agaggtactt ggcgagcacg
     6061 gcgcgcagcg gcgcgtgctc gggcgggtcg gaggcgagcg cgctgccgcg cagcgcgtcg
     6121 ttcatcatgt cgttgaagac ggccttggtg gagctgaaga tcgccggggt cgcgagcgcg
     6181 aactggatcg cgtcataccg cgtcagcgcc cagagatcgt tgcgggtcag gtgcacgacg
     6241 gcggcgctct cgcgccacgc ggcgtagtag cggtcgcggt cgctgaggac ctcctccccg
     6301 aagaagtcct ccggcgcctc ggcgacggag gtggtatgcc gctcgctgac gtccacggac
     6361 atgcgcagct ccctgggttg ccggggcctc ccggtgatgc cccttctgcg caagtcttga
     6421 gaccccgtgg cccgccccgc cagcgcaacg accggagcta ggcccgtcca gcgacggggt
     6481 tacgtacccg ctacgccttc gaaccgggca atgcccgtcg ctcgcctgat ctggtgcagc
     6541 tgcgctggtg agcgcggggt gaaatgccgg tgggccggaa cgcgattgcg ttccggccca
     6601 ccggattcgg gcggtctgcc gcgaggatca ctcctcgtcg cggcgcgact cctcgccgtg
     6661 gtgggtgcga cggcggctgc ggctgcggcc accctcgcga cgctcgc
//
LOCUS       DAOPJG010000181         6695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_12149_length_6695_cov_7.083584, whole genome shotgun sequence.
ACCESSION   DAOPJG010000181 DAOPJG010000000
VERSION     DAOPJG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12149_length_6695_cov_7.083584"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1639
                     /gene="secD"
                     /locus_tag="PKU75_13805"
     CDS             35..1639
                     /gene="secD"
                     /locus_tag="PKU75_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPF82000.1"
                     /translation="MPENPSRQQEDNLRRSSALQFRPVLQAQYTATTPQPSATVGSNE
                     TATGSATSSGSATGSASGSATSRSSSSNSSAADDATSRSTQTTASRSKRAATSTSSAS
                     ASGSASESASESASDSGSASESESGSGSATSSESPTPSGKPTSATDLAWITPELTKQF
                     DELDCSKPGVLDNLVDDATKPMVTCSTDGLEKFILGPVVVGGKDISNAVAGYQLAPNG
                     QMTNTVEIQLTFDSAAAKKYGELSIEMLALPSPQNRLASTLDSRVIVAPQFNEAIPGG
                     QASITGGFTFEQAQDLAKQLKFGALPISFDLETRSQISPTLGEEQLRLGLIAGLIGLG
                     LVVLYSLFQYHALGLVTVASIFVAALLTYLVITILGWAQNYRLDMAGVTGLIVAIGVT
                     ADSFIVYFERIRDEVREGRTLRTAVDTGWKRARRTILAADGVNFLGALVLYLLASSSV
                     RGFAFTLGLTTLIDILVVFMFTHPLVALLARRKFFAEGHKASGLDPERLGAKVRYVGR
                     GQFAGPRTATRSAVTTPTAASEGSRA"
     gene            1636..2955
                     /gene="secF"
                     /locus_tag="PKU75_13810"
     CDS             1636..2955
                     /gene="secF"
                     /locus_tag="PKU75_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPF82001.1"
                     /translation="MSKFAQFGNDLYTGKRSIDFIGRRRTWYLISAVGLVLALAGLGL
                     RGLNLGIEFTGGSEFRVSQVAIASDYEQQAQEAVHSVRPGQGVGVTKLGSDTVRVQTD
                     KLDDAQSNAVRDALARSFGTSSDKVSANFIGPSWGESVSKQALKALFVFLGLVMLVMA
                     LYFRTWKMALAALVALLHDMLFTVGIYALTGVEVSPATMIGFLTVLGYSLYDTVVVFD
                     KVRENTTEAFANRRSTFAAAANLAVNQTLVRSINTTVVALLPIAAVLVVAFLKLGPGT
                     LLDLALALFIGMAVGAYSSIFIATPFLVSMRSKDQAVLDLDKHAARVQARTTRGTDSR
                     REAAPARKAAGAAGATAPATQTSAARTSAAKTTAASGATALGATALGAAAPATGANGI
                     PADGTGITDSQDAPVDAPTGTLSGRQVHKYAQSGPRNQPRRQPKSKR"
     gene            2964..3488
                     /locus_tag="PKU75_13815"
     CDS             2964..3488
                     /locus_tag="PKU75_13815"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPF82002.1"
                     /translation="MSEISAVVSAHLRDVPDFPSPGILFKDIGPLIGDAQAFAAVTEH
                     WRLRHQHRVDAVVGIDARGFIFGGALALALGVGFVPVRKAGKLPGPTVEKSYDLEYGS
                     ATLAVQRGALEPGQRVVVIDDVLATGGTARAACELIEEVGGEVTAFEVVLELAALDGR
                     AQLSRWPVEALATG"
     gene            3622..5946
                     /locus_tag="PKU75_13820"
     CDS             3622..5946
                     /locus_tag="PKU75_13820"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPF82003.1"
                     /translation="MSEARSSSGSSTTAPIPTARSRALRRLERLAVPRSAKSNPTLEP
                     LMTLVRANHPRADTAIIERAYEVAEEAHRDQKRKSGEPYITHPLAVASILATLGMPTD
                     TVAAALLHDVVEDTDVPLEEIEREFGREIARMVDGVTKLDKVKFGEAAVSETLRKMVV
                     AMAGDIRVLVIKLADRLHNARTWQYVPPESAQRKAAETLEIYAPLAHRLGMNTIKWEL
                     EDLSFKTLYPKVYDEIKRQVAERAGERAEYLASVQKQIEADLAEARVKATVTGRPKHY
                     YSVYQKMMVGQRAFDDIHDLVALRVLVDTSRDCYAALGALHARWKPITGRFKDYIAMP
                     KFNMYQSLHTTVIGPAGRPVEVQIRTWDMHRRAEYGVAAHWKYKENKSTGRQELADGV
                     TGSLGDMAWLKQLLDWQRETTDPEDFLDSLRYEINTREVYIFTPKGDVMSLPAGSTPV
                     DFAYAVHTEVGHRTVGARVNGRLVPLESVLENGDVVEVLTSKAEGAGPSRDWLQFVRS
                     PRAKNKIRQWFSKSRREEAIDRGKDAIAKAMRKQHLPLQRMMTADSLAAVTTDLRYSD
                     IEALYAAVGENHISAQHVVARLIASFGGELGAAEDVAESANPNRTMRRRADDPGVIVK
                     GMDATDVWIKLKRCCTPVPGDQIMGFVTRGDGIAVHRVDCTNVAGLQAHPERILEVEW
                     APRSGSMFLVQIQVEALDRNHLLADITRVLSDNQVNILGAQVHTTKKRVAYSTFMFEM
                     GDPTHLEHVLNAVRNVQGVFEVTRGQGQGPSFAE"
     gene            6238..6642
                     /locus_tag="PKU75_13825"
     CDS             6238..6642
                     /locus_tag="PKU75_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82004.1"
                     /translation="MTISAKWHCIRKGDTMHARWATPVAIVAVALGGTTAALASTPAA
                     KGCTWFADAPNSNNDAAVGRKGCTNTIKANGYIKENRVGPDDIVGETLNVGAGWFMVY
                     GTCGNGQGKYFAQVTAVNSSGGARSDDVVRCN"
BASE COUNT         1142 a         2373 c         2149 g         1031 t
ORIGIN      
        1 caccacccag ggcgagaaca atatcgtcgt ctcgatcccg gagaacccga gccggcagca
       61 ggaggacaac ctgcgccgct cttcggccct ccagttccgt cccgtgttgc aggcccagta
      121 caccgcgacg acgccgcagc cctccgccac cgtcggctcc aacgaaacgg cgacgggttc
      181 ggctacgtcg tcgggcagcg cgaccggcag cgcctcggga tcggccacct cgaggtcgtc
      241 gtccagcaac agctcggccg cggacgacgc gacctcgcgc tccacccaga ccacggcgtc
      301 ccgctcgaag cgcgccgcca cctcgacctc gtcagcatcg gcctccggtt cggccagcga
      361 gtccgccagc gaatccgcct cagacagcgg gtcggcgtcg gaaagcgagt caggcagcgg
      421 gtcggcgacg agcagcgagt cgcctacccc gtccggcaaa cccaccagcg ccaccgacct
      481 cgcctggatc acgcccgagc tgaccaaaca gttcgacgaa ctcgactgca gcaagcccgg
      541 cgtcctggac aacctcgtgg acgacgcgac caagccgatg gtgacctgtt ccaccgacgg
      601 gttggagaag tttattctcg gcccggtcgt cgtggggggc aaggacatct ccaacgcggt
      661 cgccggctac cagctcgccc cgaacggcca gatgaccaac accgtcgaga tccagctcac
      721 cttcgactcc gccgcggcca agaagtacgg cgagctgtcg atcgagatgc tggctctccc
      781 gagcccgcag aaccggctcg cctcgaccct cgactcccgc gtcatcgtgg ctccgcagtt
      841 caacgaggcc atcccgggcg ggcaggcctc gatcaccggt ggcttcacat tcgagcaggc
      901 gcaagacctg gccaagcagt tgaagttcgg tgccttgccg atctccttcg atctcgagac
      961 ccgcagccag atcagcccca ccctcggtga ggagcaactg cggctcggtc tgatcgccgg
     1021 gctcatcggt ctgggcctgg tcgtgctcta ttcgctgttc cagtaccacg ccctcggcct
     1081 cgtcaccgtc gcctccatct tcgtggctgc gctgctgacc tacctcgtca tcacgatcct
     1141 cggctgggcc cagaactacc gcctcgacat ggccggcgtg acgggtctga tcgtcgccat
     1201 cggtgtcacc gccgactcgt tcatcgtcta cttcgaacgc atccgggacg aggtgcgaga
     1261 gggccgcaca ctgcgtacgg cggtcgatac cggctggaag cgtgcgcgcc gcaccatcct
     1321 ggccgccgac ggcgtcaact tcctcggggc gctcgtcctc tatctgctgg ccagttccag
     1381 cgtccgcggg ttcgccttca cactcggcct gacgaccctc atcgacatcc tcgtggtgtt
     1441 catgttcacc caccccctgg tcgcattgct cgcccgccgc aagttctttg ccgaaggcca
     1501 caaggcgtcg gggctggacc ccgagcggct gggtgcgaag gtgcggtatg tcggccgggg
     1561 ccagttcgct ggtccccgca ccgccacccg ctccgccgtg accaccccga ccgccgcgtc
     1621 cgaagggagc cgggcatgag caagttcgcc cagttcggaa acgacctcta caccggcaag
     1681 cggtccatcg acttcatcgg ccggcggcgc acgtggtatc tcatctccgc cgtcggcctg
     1741 gtcctcgcgc tcgccggtct cggcctgcgc gggctgaacc tcggcatcga attcaccggc
     1801 ggctcggagt tccgggtctc gcaggtcgcc atcgcctccg actacgaaca gcaagcccaa
     1861 gaggcggtcc actcggtccg tcccgggcag ggggtcgggg tcaccaagct cggcagcgac
     1921 acggtgcggg tgcagaccga caagctcgac gacgcccaga gcaatgcggt ccgagacgcc
     1981 ttggccaggt ccttcggcac ctccagcgac aaggtctcgg ccaacttcat cggcccctcg
     2041 tggggggagt cggtcagcaa gcaggccctc aaggccctct tcgtcttcct cgggctcgtc
     2101 atgctggtca tggcgttgta cttccgcacc tggaagatgg cactggccgc gctcgtcgcc
     2161 ttgctgcacg acatgctctt caccgtcggc atctatgccc tcacgggtgt ggaggtctcg
     2221 ccggcgacga tgatcggctt cctgaccgtc ctcggctact cgctctatga cacggtcgtc
     2281 gtcttcgaca aggtccgcga gaacaccacc gaggcattcg ccaaccgccg cagcaccttc
     2341 gccgcggccg ccaacctggc cgtgaaccag accctggtcc gctccatcaa caccaccgtc
     2401 gtcgcgctcc tgccgatcgc cgccgtgctc gtggtcgcct tcctcaagct cggcccgggc
     2461 accctgctcg acctggccct cgcgctcttc atcggtatgg ccgtcggcgc ctactcctcg
     2521 atcttcatcg ccacgccgtt cctcgtgagc atgcgcagca aggaccaggc ggtcctcgac
     2581 ctcgacaagc acgccgcacg cgtgcaggcc aggaccacgc gtggcaccga ctcccgccgc
     2641 gaagctgcac ccgcgcggaa agccgccggc gcggccggcg ccacggcgcc cgcaacccag
     2701 acctcggccg ccaggacctc tgccgccaag accacggcag cgtccggggc cacagcgctc
     2761 ggggccacag cgctcggggc cgcagccccg gcgaccggag cgaacggcat accggcggac
     2821 ggcaccggga tcaccgactc gcaagacgcc ccggtcgacg cgcccacggg aaccctcagc
     2881 ggccggcagg tgcacaagta cgcccagtcc ggcccgcgca accagccccg ccggcagccg
     2941 aaatccaagc gctgaacacc acgatgagcg agatctccgc ggtcgtgagt gcgcatctgc
     3001 gcgacgtccc cgacttcccc agccccggga tcctgttcaa ggacatcggc ccgctgatcg
     3061 gggatgcgca ggccttcgcc gcggtgaccg aacactggcg gctgcgccat cagcaccggg
     3121 tcgacgcggt cgtcgggatc gacgcgcgcg ggttcatctt cggcggcgca ctggctctcg
     3181 cgctcggcgt cgggttcgtc ccagtgcgga aggcgggcaa gctgcccggg ccgacagtcg
     3241 agaagtccta cgacctcgaa tacggcagcg cgaccttggc ggtgcaacgc ggcgccctcg
     3301 agccggggca gcgggtcgtg gtgatcgacg acgtgctggc taccggcggg accgcccggg
     3361 ccgcgtgcga gctgatcgag gaggtgggcg gcgaggtgac ggctttcgag gtcgtcctcg
     3421 aactggccgc gctcgacggc agagcacaac tgtcgcgatg gcccgtggag gccctggcga
     3481 ccggctgacc ccgccgccct gaccccgccg ccctccgtcg agatgtcgat tcggccccgt
     3541 cgagatgtcg attcccgcga tagccaactg cacggcatac ccagattcga gttgacccga
     3601 agggtcgact aacctctgtc catgagcgag gctcgcagct ccagcggatc gagcaccacc
     3661 gcgccgatcc ccactgcacg cagccgcgcc cttcgccgac tcgagcggct cgccgtcccg
     3721 cgcagcgcca agtccaaccc gacactcgaa cccttgatga cgttggtgcg cgccaaccat
     3781 ccgcgcgccg acaccgccat catcgagcgc gcctacgagg tcgccgagga agcccaccgc
     3841 gatcagaagc gcaagtccgg tgagccctat atcacccacc cgctggccgt cgcgtccatc
     3901 ctcgccacgc tgggaatgcc caccgacacg gtcgcggcgg cgctcctgca cgatgtcgtc
     3961 gaggacaccg acgtcccgct cgaggagatc gagcgcgagt tcggccgcga gatcgcgcgg
     4021 atggtcgacg gggtcaccaa gctggacaag gtcaagttcg gcgaggcggc ggtctccgag
     4081 acgttgcgca agatggtcgt cgccatggcg ggcgacatcc gggtcctcgt gatcaagctc
     4141 gccgacaggc tgcacaacgc ccggacctgg cagtacgtcc cgcccgagtc ggcccagcgc
     4201 aaggcggccg aaacgctcga gatctatgcc ccgctggcgc accggctcgg catgaacacg
     4261 atcaagtggg aactggagga cctgtccttc aagaccctct atccgaaggt ctacgacgag
     4321 atcaagcgcc aggtggccga gcgcgcgggg gagcgcgccg aatatctggc gagcgtgcag
     4381 aagcagatcg aggccgacct ggccgaggcc cgggtcaagg ccaccgtgac cggccggccg
     4441 aagcactatt acagcgtcta ccagaagatg atggtcggcc agcgcgcctt cgacgacatc
     4501 cacgacttgg tggcgttgcg ggtgctggtc gacacctccc gcgattgcta tgcggcgctc
     4561 ggggcgctgc acgcccggtg gaagccgatc acggggcggt tcaaggacta catcgcgatg
     4621 ccgaagttca acatgtacca gtcgttgcat acgacggtca tcgggccggc ggggcggccg
     4681 gtggaggtgc agatccgcac ctgggacatg caccggcgcg cggagtacgg cgtggcggcg
     4741 cactggaagt acaaggagaa caagtccacc ggtcgccagg agttggccga cggtgtcacc
     4801 gggtcgctcg gcgacatggc gtggctgaag cagcttctcg actggcagcg ggagacgacc
     4861 gaccccgagg acttcctcga ctcgttgcgc tacgagatca acacccgcga ggtctacatc
     4921 ttcacgccga agggcgacgt catgtcgctg ccggcgggct cgacgccggt ggactttgcg
     4981 tatgccgtgc acaccgaggt cggccaccgc accgtcggcg cccgggtcaa cggccggctg
     5041 gtgccgctgg agtccgtgct cgagaacggt gacgtcgtcg aggtgctcac ctccaaggcc
     5101 gagggcgccg gcccgtcacg ggactggctg cagttcgtcc gctcaccgcg cgcgaagaac
     5161 aagatccgcc agtggttctc caagtcccgc cgcgaggagg ccatcgaccg cggcaaggac
     5221 gcgatcgcca aggcgatgcg caagcagcat ctgccgctgc agcggatgat gacggccgac
     5281 tcgctcgctg cggtgacgac ggatctgcgc tattcggaca tcgaggcgct gtatgccgcg
     5341 gtgggggaga accacatttc cgcccagcac gtcgtggcgc gcctgatcgc cagcttcggt
     5401 ggggagttgg gggccgccga ggacgtcgcc gagtccgcca atcccaaccg gaccatgcgc
     5461 cggcgcgccg acgacccggg cgtcatcgtc aagggcatgg acgccaccga cgtctggatc
     5521 aagctcaagc ggtgctgcac cccggtgccc ggggaccaga tcatgggctt cgtcacgcgc
     5581 ggcgacggca tcgcggtcca ccgggtcgac tgcaccaatg tcgctgggct gcaagcgcat
     5641 ccggagcgga tcctcgaggt cgagtgggcg ccgcggtcgg gctcgatgtt cctcgtgcag
     5701 atccaggtcg aggcgctcga ccgcaaccac ctgctcgccg acatcacccg ggtgctctcg
     5761 gacaaccagg tcaacatcct cggcgcccag gtgcacacga ccaagaagcg cgtcgcctac
     5821 tcgaccttca tgttcgagat gggcgacccg acccacctcg agcacgtcct caacgccgtc
     5881 cgcaacgtcc agggcgtctt cgaggtcacc cgcggccagg ggcagggccc gtccttcgct
     5941 gagtagagtg gcccgatggg tgccagcgcg ggcctgcccg agaagaggcc actatctcac
     6001 cggtctccga ctcaagttgc ccctgctcgc tgagctgccc aagggcgaag aatcactgcg
     6061 cgcggtagtc gtcgcgtgcc gggcggacat ggtgaagcga aacgcgtaag ccggccgctg
     6121 ctggcgtcaa gattctcctt tgcaacgtct gtcgcatgac gctctcggcc tcgcaggttg
     6181 gacgcgtcct agtccgtcgc cgggttcggg ttgcggcctt cccgagaggg gcctagggtg
     6241 acgatcagtg ccaagtggca ctgcatacgg aaaggggaca cgatgcatgc acgttgggcg
     6301 acgcccgttg caatagttgc agtggcgttg ggaggcacta ccgcggcact ggcttccact
     6361 cctgcggcta agggctgcac ttggttcgct gacgcgccga acagcaacaa cgacgccgct
     6421 gtgggtcgta agggctgtac caacaccatc aaggccaacg gctacatcaa ggaaaaccgc
     6481 gtcggccctg atgacatcgt gggtgagaca ctgaatgtcg gagcagggtg gtttatggtt
     6541 tacggcacct gcgggaatgg gcagggaaag tacttcgcac aagtcacagc agtcaatagc
     6601 agcggcggtg ccagatcgga cgacgttgtt cgttgcaact agcataactc gaacagaagt
     6661 gcggtgctgt cgggcatcta gccatggtgt ccgac
//
LOCUS       DAOPJG010000182         6511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_12617_length_6511_cov_5.863693, whole genome shotgun sequence.
ACCESSION   DAOPJG010000182 DAOPJG010000000
VERSION     DAOPJG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12617_length_6511_cov_5.863693"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PKU75_13830"
     CDS             <1..956
                     /locus_tag="PKU75_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPF82005.1"
                     /translation="AGLVSGQVVLVTGAGGSIGSELSRQVHRLQPARLILLDRDESAL
                     HALELSLFGTGLLNRDEMVLCDLRDESALAAVFELHQPDVVFHAAALKHLPMLERFPA
                     EGWKTNVLGTLHVLRCAARFGVRHVVNVSTDKAADPTSVLGRTKRLAERLTAHYAVVT
                     GEPWVSVRFGNVLGSRGSVLETFRAQIDQGGPVTVTHPDVTRYFMTIPEACELVLQAA
                     TLGDRGEVLVLDMGRPVRIVDVAKRLIAESRSDISIEYTGLRHGEKLHEVLLSDLEVA
                     EPSSHERITRVVIEGMDPQIVEGMCPGTQTSLTADREHQTH"
     gene            956..2245
                     /locus_tag="PKU75_13835"
     CDS             956..2245
                     /locus_tag="PKU75_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPF82006.1"
                     /translation="MSAPLPTGDPTVAVIGLGYVGLPLAVAFGRELKVIGFDIDGERV
                     RQLAAGHDRTLEIDAEELAAARHLIFTDRAADLATADVYIVTTPTPVDASNRPDLTPI
                     LSASKTVAAVLSRGDVVIYESTVYPGATEEECVPVLEKGSGLRLDVDFSVGYSPERIN
                     PGDKEHRVTNIVKVTSGSTPEAAIFVDDLYRRVVTVGTHCAPTIKVAEAAKVIENTQR
                     DVNIALMNELAILFARLDIDTEDVLVAAGTKWNFLGFRPGLVGGHCIGVDPYYLTHKA
                     QMVGYHPEVVLAGRRLNDGMGAHVAAQLVKAMTKRGIPVQGARVLVLGLTFKENTPDL
                     RNTRVIDVIRELEEFDVAVDVHDPWVDAESAAEHYGVQMVEVPEPGAYDAVVLAVAHR
                     EFREWGSDHIRALGKPGAHVLYDLKYVLDRADSDLRL"
     gene            2242..3399
                     /locus_tag="PKU75_13840"
     CDS             2242..3399
                     /locus_tag="PKU75_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF82007.1"
                     /translation="MSERIHLSSPDVTEAEERALVRAIRSGWVAPLGPEVDAFEEELA
                     AYCGREHAVALSSGTAALHLGLITLGVGPGDLVLTSTMTFAATANAITYVGAEPVFVD
                     CDATGNMDAGLLEEAFERCAGGGQRVAAVLPVDLLGKVVDHARVDDIASAHGVPVLAD
                     AAESLGATRDGRPAASFGNVAAVSFNGNKVMTTSGGGALLTDDAHLAQRVRYLATQAR
                     QPVVHYEHTEIGYNYRLSNLLAALGRPQLARLDDMVQRRRQHRLGYLELFDGVAGVSA
                     FGEPSGLAGGPTHDNWWLTSVIIDPSVAGFAPEDLRLALSDADIEARPLWKPMHLQPV
                     FAGARAVLDGTSERLFSTGLSLPSGSVLDDGQMQRIHAVITAFLEGRRATR"
     gene            3386..4027
                     /locus_tag="PKU75_13845"
     CDS             3386..4027
                     /locus_tag="PKU75_13845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPF82008.1"
                     /translation="MPHVERRRYDVAKRAIDVTVSAVGLVLSAPVQLVTAAVVLAAHG
                     RPVLFRQPRPGRDGVVFELVKFRTMRRPDAAHVTDADRLTSVGRILRSTSLDELPTLW
                     NVLKGDMSLVGPRPLLVEYLPRYSPEQARRHEVRPGVTGLAQVSGRNGVAWEDKFALD
                     VEYVDTRSLTLDLVILARTVCCVVKRQGISGGGEATMSVFMGSPAVRVGDDGA"
     gene            4014..4688
                     /locus_tag="PKU75_13850"
     CDS             4014..4688
                     /locus_tag="PKU75_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HPF82009.1"
                     /translation="MTAPDGVGLERVVVVGASGFGRECLDVLEAMAAAGSPVEVVGVV
                     DDGPSELNMERLASRGVAYLGTVDEWLAGDAVTRRYVLGIGSPAVRRRLAARLEGTGA
                     RPFTAIHPSATFGARVAKGEGAVVCAGVAVSTNVQLGRHVHLNPNATLGHDAILGDFL
                     SVNPGAVVSGEVTIGAGTLVGASATILQGLRVGEDVVVGAGSVVTRDVPAAAVVKGVP
                     GQWDPT"
     gene            4685..5722
                     /locus_tag="PKU75_13855"
     CDS             4685..5722
                     /locus_tag="PKU75_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82010.1"
                     /translation="MTDFSSHSATRSSPFARRRASGELLLHPVVSPRYYRYYMDGLEA
                     VYGRTPTLSTRGFPELRDPTEGMAVILPDGLRLFIAANDFAVVDPGVVAWADVVGQVN
                     VDPAISYSPKVIPIGPSFGLPWRSRPSLASFVLRSGAMAAPSRVPAMLRDYLRAHGDR
                     APLSEFRTSLSTDSEVFFIAEYWNNAPAANERRLRFVRAVRRRPSLTLSGGFRSREDL
                     PAGFAQFRLETPIPHREYLRRTKESAFVFNTPAVHDCLGWKLGEFLALGKAIISTPLG
                     RTMPGDFRSGEQAHIVDGSEESILAAIDLLCEDRAYRRHLEESARRYWEQYLRPEVVI
                     RRMVHSAGGPG"
BASE COUNT          973 a         2186 c         2219 g         1133 t
ORIGIN      
        1 tcgctggcct ggtgagcggc caggttgtgc tcgtcaccgg tgccggcggt tcgatcggat
       61 cggagctgag ccggcaggtg caccggctcc agcccgctcg cctgatcctg ttggatcgcg
      121 acgaatcagc cctgcatgcc ctcgagctct cgctcttcgg caccggcctg ctcaaccgcg
      181 atgagatggt cctgtgcgac ctgcgtgacg agtcagcact ggcggcggtc ttcgaactcc
      241 accagcccga tgtcgtcttc catgccgcgg cgttgaagca cctccccatg ctggagcgct
      301 tcccggcgga gggatggaag accaatgtgc tcggcaccct gcacgtcctc cggtgcgcag
      361 cccggttcgg cgtgcgacac gtcgtcaacg tctcgaccga caaggccgcc gacccgacga
      421 gcgtgctcgg ccggaccaag cggctcgccg agcgtctgac cgcgcactat gcggtcgtga
      481 cgggggaacc ctgggtctcg gtccgcttcg gcaacgtgct cggctccaga gggtcggtcc
      541 tggagacgtt ccgcgcgcag atcgaccagg gcgggccggt cacggtgacc caccccgacg
      601 tcacgcgcta cttcatgacc attcccgagg cctgcgagct cgtcctgcaa gccgcgacgc
      661 tcggtgaccg tggtgaggtc ctcgtcctcg acatgggccg tccggtgcga atcgtcgacg
      721 tggccaagcg gttgatcgcg gagtcccgca gtgacatctc gatcgagtac accgggcttc
      781 gtcatggcga gaagttgcac gaggtcttgc tcagcgacct cgaggtcgcc gaaccgagca
      841 gtcacgagcg catcacccgg gtcgtcatcg agggcatgga tccgcagatc gtcgagggca
      901 tgtgccccgg aacccagacg tcgctcacgg ctgaccgcga gcaccagacc cactgatgag
      961 cgcaccactc cccaccggtg accccacggt cgccgtcatc ggcctcggtt atgtcggcct
     1021 gccgctcgcg gtcgccttcg gtcgcgagct caaggtcatc ggctttgaca tcgacggcga
     1081 gcgcgtgcgc caactggccg ccggccatga tcgaacattg gagatcgatg ccgaggagct
     1141 cgccgctgct cgtcacctga tcttcaccga tcgcgcggcg gacctggcga ctgcggacgt
     1201 ctacatcgtc acgacgccga cgccggtcga tgcttccaac cgccccgacc tgacgccgat
     1261 cctgtctgcg tccaaaacgg ttgcggccgt gctctcgagg ggcgatgtcg tcatctacga
     1321 gtcgaccgtg taccccggcg cgactgagga ggagtgtgtc ccggtcctcg aaaagggatc
     1381 gggcctgcgc ctcgatgtcg atttctcggt cggttacagc ccggagcgca tcaatcccgg
     1441 ggacaaggag catcgcgtca cgaacatcgt caaggtgacg tccgggtcga ctccggaggc
     1501 tgccatattc gtcgatgacc tctatcgccg ggtcgtcacg gtcggcaccc actgcgctcc
     1561 gaccatcaag gtcgccgagg ccgccaaggt catcgagaac acccagcgcg acgtcaacat
     1621 cgcgctgatg aacgagctcg ccatcctctt cgctcggctg gacatcgaca ccgaggacgt
     1681 cctcgtggcg gccgggacga agtggaactt cctcggcttc cgccccggcc tcgtcggtgg
     1741 ccactgcatc ggcgtcgacc cctactacct gacccacaag gcgcagatgg tgggctacca
     1801 ccccgaggtg gtcctcgccg gccgacggct caacgacggc atgggggccc atgtcgccgc
     1861 ccagctcgtc aaggcgatga ccaagcgcgg catcccggtg cagggcgcgc gcgtgctcgt
     1921 gctcggcctg acgttcaagg agaacacgcc cgacctgcgc aacacccggg tcatcgacgt
     1981 catccgcgag ctcgaggagt tcgacgttgc ggtcgatgtg cacgacccct gggtggacgc
     2041 ggaatcggcg gccgagcact acggtgtgca gatggtcgaa gtccccgagc ccggtgccta
     2101 cgacgcggtg gtcctcgccg tggctcaccg tgagttccgt gagtggggga gcgaccacat
     2161 ccgggcactc ggcaagcccg gcgcccacgt gctctacgac ctcaagtacg tcctggaccg
     2221 ggccgactcg gacctgcgcc tgtgagcgag cgcatccacc tctcgtcccc tgacgtgacc
     2281 gaggccgagg agcgggcctt ggtgcgggcg atccgctcag gctgggtggc accccttggc
     2341 cccgaggtcg acgcgttcga ggaagagctc gcggcctact gcgggcggga gcacgccgtg
     2401 gcgctgtcgt cgggcacggc cgcgttgcac ctgggcctga tcacgctcgg cgtcggtccc
     2461 ggcgatctcg tcctcacgtc gacgatgacg ttcgccgcga ccgcgaacgc aatcacctac
     2521 gtcggcgcgg agccggtctt cgtcgactgc gatgcaaccg gcaatatgga tgccgggctc
     2581 ctcgaggagg cgttcgagcg gtgcgcgggc ggtgggcaac gggtcgcagc ggtgctgccg
     2641 gtcgacctgc tgggcaaggt cgtggaccac gcgcgggtcg acgacatcgc ctcagcccat
     2701 ggggtcccag tgctcgcgga tgccgcggag agcctcggtg cgacccgcga cggccgcccg
     2761 gcagcctcgt tcgggaacgt ggcggccgtg tcgttcaacg gcaacaaggt gatgacgacc
     2821 tccggcgggg gggcgctgct caccgacgac gcgcacctcg cacaacgggt gcgctacctc
     2881 gccacccagg cacgccagcc ggtcgtgcac tacgagcaca ctgagatcgg ctacaactac
     2941 cgactctcga atctcctggc cgccctcggc cgcccccagc tcgcccgcct cgacgacatg
     3001 gtgcagcggc ggcgccagca ccggctgggc tacctcgagc tgtttgacgg ggtggccggg
     3061 gtgagcgcct tcggtgagcc gtcgggtctg gcgggcgggc cgacccacga caattggtgg
     3121 ctgacgtcgg tgatcatcga cccgtcggtg gccgggttcg ctcccgagga cctgcggctg
     3181 gccctcagcg acgccgacat cgaggcgcgt ccgctgtgga agccgatgca cctccagccc
     3241 gtgttcgcgg gtgcccgagc cgtgctcgac ggcacgagcg agcggttgtt ctcgaccggc
     3301 ctgtcgctgc cgagcggctc ggtactggac gatgggcaga tgcagcgcat ccacgcggtc
     3361 atcaccgcct tcctggaggg tcgccgtgcc acacgttgag cggcggcgct acgacgtcgc
     3421 caagcgggcg atcgacgtca cggtcagcgc cgtcgggctc gtcctgagcg ccccggtgca
     3481 gctcgtcacc gccgcagtgg tgctcgcggc ccacgggcgc ccggtgctct tccgccagcc
     3541 ccgcccgggt cgcgacggcg tcgtgttcga gctcgtgaag ttccgcacga tgcggcgtcc
     3601 ggacgccgcc cacgtcacgg atgccgaccg cctcacttcc gtgggtcgga ttctgcgctc
     3661 aaccagcctc gacgagctgc cgacgctgtg gaacgtgctc aagggcgaca tgagcttggt
     3721 cgggccgagg ccgctgctcg tggagtacct gccgcgatac agccccgaac aggcgcgacg
     3781 ccacgaggtg cgtccggggg taactggcct ggcccaggtg tcggggcgca acggtgtggc
     3841 ctgggaggac aagttcgctc tcgacgtgga gtacgtggac acccggagcc tgacgcttga
     3901 tctggtgatt cttgctcgca ctgtttgctg cgtggtgaag cggcagggga tcagcggtgg
     3961 gggcgaggcg acgatgagcg tgttcatggg gtcgccggcg gtacgggtcg gcgatgacgg
     4021 cgcctgacgg ggttggcctt gaacgcgtcg tcgtggtggg ggcatcgggc ttcggtcggg
     4081 agtgcctcga cgtgctggag gccatggcag cggccgggtc acctgtcgag gtggtcgggg
     4141 tggtcgacga cgggccctcc gagctcaaca tggagcggct cgcgtcgcgc ggtgtcgcct
     4201 acctgggcac cgtcgatgag tggctggctg gggatgccgt cacccggcgg tacgtgctcg
     4261 gtatcggcag cccagcggtg aggcggcggt tggccgctcg gctcgagggc accggggcgc
     4321 gacctttcac ggccatccac cccagcgcga cgttcggcgc tcgtgtcgcc aagggggagg
     4381 gggccgtcgt ctgcgcgggg gtggcggtgt cgacgaacgt ccagttgggc cggcacgtcc
     4441 acctcaaccc caacgcgacg ctcggacatg acgctattct cggcgacttc ttgtccgtca
     4501 accccggggc cgtggtctcg ggtgaggtga ccatcggtgc agggacgctc gtcggcgcga
     4561 gcgctacgat cctccagggc ctgcgcgttg gcgaggacgt cgtggtcgga gccggttccg
     4621 tcgtgacgcg tgacgtccca gctgccgcgg tcgtcaaggg tgtgccgggt cagtgggacc
     4681 caacatgact gacttctctt cgcactcggc cacacggtcc tctcccttcg cgcggcggcg
     4741 cgcgtcgggc gaactgctgc tccaccccgt cgtgagccct cggtactacc gctactacat
     4801 ggatggcctc gaggccgtct acggtcggac gccgacactc agtaccagag gatttcccga
     4861 gcttcgtgac cccacggagg gcatggccgt catccttcct gatgggctgc gtctcttcat
     4921 cgcggccaac gactttgcgg tggtcgaccc aggtgtcgtg gcttgggcgg atgtcgtggg
     4981 tcaggtcaac gtcgatcccg caatctcata ctcgcccaaa gtcattccga tcggcccgag
     5041 tttcgggctt ccgtggcgat cccggccgtc cctggcctcc ttcgtccttc ggtcgggcgc
     5101 gatggctgcc ccgtcccgcg tgccggctat gctccgtgac tacctgcggg cccacggaga
     5161 tcgagcaccc ctcagcgaat ttcgtacctc cctgagtact gacagtgagg tcttcttcat
     5221 tgcggagtat tggaacaatg ccccggctgc caacgaacgc aggctgcgct tcgtccgcgc
     5281 tgtgcgacgg cggccaagcc tcaccctcag tggggggttt cggagccggg aggacctccc
     5341 agctggcttc gcccagttcc gccttgagac gcccatcccc caccgggagt atctgcgccg
     5401 cacgaaggag tcggctttcg tcttcaacac gccggccgtc cacgactgcc tgggctggaa
     5461 actgggggag ttcttggctc tcggaaaggc catcatctcc acgcccctcg ggcgcacgat
     5521 gccaggcgat tttcgctcgg gcgagcaggc ccacatcgtc gatgggtcgg aggagtccat
     5581 cctcgcggcc attgacctcc tgtgcgagga tcgagcgtac cgtcgacatc ttgaggagtc
     5641 tgctcggcgc tactgggagc agtacctccg tccggaggtc gtgattcgac ggatggtcca
     5701 ctcggcggga ggccctggat gactcacgag ctcaatcggg gcggtgctcg cgacaacgcg
     5761 cgtctgagcc tgttcgggca gccgcgggcc gggagggaag gcgtggtctt cgaactcgtg
     5821 aggtccacaa ccagtgaaat ccctatgcca cgcatgagac gatctcgacc gccttcctcc
     5881 gtgagtcggt tcccgagcct gtaccagttg tcgaccctgt ggaacgtgct caagggcgac
     5941 agagcatcgg tgcgcccaag cggctgcacg tggagtccca gccgaggtac tgcccgagca
     6001 ggcgacatga ggttggccca ggcgtcgggg agcaaggggc cctcctggga gagaaggatc
     6061 gccctcggcg tggagtgcgt gtccggccgg ggcctccacc ttcgatctgg agatcctctt
     6121 gcgtactatc catcttgtgc tcaggaggca gggaatcagc ggcaagggcg aggcgacgat
     6181 gagcgtgttc acgtcacctc acggtgatcg ggcgctcaat ggcgtcgcct gaacaggtcc
     6241 tcgtgcttgg ggcgcccgat tctatccggg aattactcga gttgctgcag gccatgtccg
     6301 ctgctggatc gcccatcgat gctccctgcg tcgggggcga cgatcagtcc gattcgaacc
     6361 gcgcgcttgc aggacgtgga ctcgcctgct tgggtaccgg ctcgccacgt tcggatcagt
     6421 caacccaagg gctaccgttt tgggccaggt aacgatcggt acgggagctc gtctcggagg
     6481 gagtgccacc ctcttccaga accagttcgt a
//
LOCUS       DAOPJG010000183         6487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_12687_length_6487_cov_7.864428, whole genome shotgun sequence.
ACCESSION   DAOPJG010000183 DAOPJG010000000
VERSION     DAOPJG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12687_length_6487_cov_7.864428"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PKU75_13860"
     CDS             complement(<1..836)
                     /locus_tag="PKU75_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF82011.1"
                     /translation="MTLTEAEVADLARRLPTPRHENFYFVMTDRFANGDKTNDKGGFT
                     GGVAETGFDPTDKGMFHGGDLAGLMSKLDYIKGLGTTAIWLTPSFQNRPTQGPEGELS
                     AGYHGYWITDFTRIDSHLGTNAEMKELIAAAHAKGMKVYFDIVTNHTADVIDYKDPGD
                     YSYISKEDKPYADASGKAFDDAPLAGGDSFPALDEKSFPYTPVFNSKADAKAKTPAWL
                     NDPTLYHNRGNSTFEGESSTYGDFFGLDDLFTENPKVVDGMIEIYKSWVDFGIDGFRI
                     DTV"
     gene            complement(1045..2757)
                     /locus_tag="PKU75_13865"
     CDS             complement(1045..2757)
                     /locus_tag="PKU75_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF82012.1"
                     /translation="MGHVDIAGVEYWLPDGRPLLRDVNLRVGEGSKTALIGPNGTGKT
                     TLIRILAGDLVPDDGTVVRSGGLGVMRQFIGQVRDDSTVRDLLLLAAAEPIRKAAAEI
                     DRTELLMMERDSEADQLAYAQALVDWGDVGGYEIETLWDECTMAALGMPFEKAQWRKV
                     TTLSGGEQKRLVLEALLRGPEEVLVLDEPDNYLDVPGKRWLEERLRTSPKTVLFISHD
                     RELLSQSATRIATLEPNSAGATIWVHPGSFTTYDQARQERNDRLDELLRRWDEEHQKL
                     KDLVQMYKVKAAYNDGLASRYQAAKTRLAKFEEAGPPEKVPLRQNVRMRLAGGRTAKR
                     AVVCERLELQIASPDGGSGTQLMKPFDAELWFGERIAILGSNGSGKSHFLRLLAAGGS
                     DPDIEHRPVGEFAPTPVAHTGIARLGSRVRPGWFAQTHDHSALHGRTLLEILHRGDEH
                     RAGKPREEAAKALDRYGLAMAGERTFEMLSGGQQARFQILLLELSGATMLLLDEPTDN
                     LDLHSAEALEEALDAFEGTVVAVTHDRWFARQFDRFLVFGADGRVYESADPVWDEARV
                     QRRR"
     gene            complement(2814..3215)
                     /locus_tag="PKU75_13870"
     CDS             complement(2814..3215)
                     /locus_tag="PKU75_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF82013.1"
                     /translation="MDKPQLFHCLTFTDVDRAIDFLTAIGFTEVVVVRDPDDPATVVH
                     AQFRWRDHGGLMFGSVRHDDTLGFASRPGVGACNLVVTSDAEVDRVLTAALDHGATLA
                     QEPHHPPHGGRSAAVRDAEGNLWNIDSYPGE"
     gene            3237..3587
                     /locus_tag="PKU75_13875"
     CDS             3237..3587
                     /locus_tag="PKU75_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82014.1"
                     /translation="MSWVLEKWEVSRDAGPDMHMLMCIDMSALERRLHLLLDQDRHDR
                     LVAEAERTGRSVNAVIRAAIDEHFDEQRDQVRRAAAARLLLASAAAPGSEPAETWEQM
                     LAAQDADLQDSLRP"
     gene            3584..3997
                     /locus_tag="PKU75_13880"
     CDS             3584..3997
                     /locus_tag="PKU75_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPF82015.1"
                     /translation="MIDLFVDSPVFLHALGAPVAVTPACQALIRSAQENTVRLHTAVE
                     CVQEIAFHRTRMGDVSRALAQVNAIRDLCVVHPFDDRVLDRGLELMAATTIRGRDAFI
                     AATALLAGFESIVTTDARFVEVPGLRPAGPGSWTA"
     gene            complement(3994..4992)
                     /gene="meaB"
                     /locus_tag="PKU75_13885"
     CDS             complement(3994..4992)
                     /gene="meaB"
                     /locus_tag="PKU75_13885"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPF82016.1"
                     /translation="MREIDVAALTADVLSGSRAAIARAITLVESSREDHRSKASELIG
                     ALTPHTGSSVRVGITGVPGAGKSTFINALGTRLIQRGNRVGVVAVDPSSRRTGGSILG
                     DRTRMAELTTSDHAFVRPSPSGRHLGGVARATRESMLVLEAAGYDVIIVETVGVGQNE
                     IAVSEMVDTFLLLTLAGAGDQLQGIKRGILELADVIAVNKADGDGEGRARVAARDLAT
                     AMRLMLPGKDVRRPPVLTCSAATHSGLDEVWQAVLDHRGHLEAEGSLATRRAQQQQDW
                     MWAMVDAHLEDAVRHTHSVREQRAALESAVRAGSLSAMEAADRILALYAADVRDAN"
     gene            complement(4985..>6487)
                     /gene="scpA"
                     /locus_tag="PKU75_13890"
     CDS             complement(4985..>6487)
                     /gene="scpA"
                     /locus_tag="PKU75_13890"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPF82017.1"
                     /translation="SQHMPKFNSISISGYHMQEAGATQDLELAYTLADGVEYLRAGLG
                     AGLTVDQFAPRLSFFWAIGMNFFMEVAKMRAARLLWAKLVKQFNPKSDKSLSLRTHSQ
                     TSGWSLTAQDVFNNVVRTGIEAMASTQGHTQSLHTNALDEAIALPTDFSARIARNTQL
                     VIQQESGTCRTIDPWAGSAYVEKLTHDLARRAWEHIEEVEAAGGMAKAIEAGIPKMRI
                     EEAAARTQARIDSGQQPVIGVNRYVVEDDEEIDVLKVDNAAVRAQQIAKLERLRAERD
                     QAEADAALAKITEACSKDGGRDLSSNLLALAIDAARAKATVGEISEAMEKSFGRYTAT
                     IRTIGGVYSAEAGDDEGVHAAQQLVEEFEEAEGRRPRILVAKMGQDGHDRGQKVIATA
                     FADLGFDVDVGPLFQTPEEVARQAVEADVHIVGVSSLAAGHLTLVPALRQELDKLGRE
                     DLMIVVGGVIPSQDFAELRAAGADAIYPPGTVIAAAAQDLIRDLRARLDA"
BASE COUNT          977 a         2224 c         2198 g         1088 t
ORIGIN      
        1 acggtgtcga tccggaagcc gtcgatgccg aagtcgaccc agctcttgta gatctcgatc
       61 atcccgtcga ccaccttggg gttctccgtg aagaggtcgt cgagaccgaa gaagtcgcca
      121 taggtcgacg actcgccctc gaaggtcgag ttgccgcggt tgtggtagag cgtcggatcg
      181 ttgagccacg caggcgtttt cgccttggca tccgccttgg agttgaagac gggtgtgtag
      241 gggaaggact tctcgtccag cgccgggaag ctgtcaccac ccgcgagcgg cgcgtcgtcg
      301 aaagccttgc ccgacgcgtc ggcatatggc ttgtcctctt tggagatata ggagtagtcg
      361 cccgggtcct tgtagtcgat gacgtcggcg gtgtggttgg tgacgatgtc gaagtagacc
      421 ttcatcccct tggcgtgagc ggcggcgatg agctccttca tctccgcgtt ggtgcccagg
      481 tgggagtcga tcctcgtgaa gtcggtgatc cagtacccgt gatagccggc gctcagctcg
      541 ccctcggggc cttgcgtggg ccggttctgg aaggacggcg tcagccagat ggccgtcgtc
      601 ccaaggccct tgatgtagtc gagtttggac atcaacccgg cgaggtcgcc gccgtggaac
      661 atgcccttgt ccgtggggtc gaatcccgtc tcggcgacac caccggtgaa accgcccttg
      721 tcatttgtct tgtccccgtt ggcgaaacgg tcggtcatga cgaagtagaa gttctcgtgc
      781 cgcggcgtcg gcaagcggcg ggccaggtcg gcgacttccg cctcggtgag ggtcaccggc
      841 ccgccgccgg aggccacctg ccccgacgcc cctgcggatc ccgaccccga ggcgccgggc
      901 ctggctccgt cgccgccacg ggtggtcacg gcatacgcga cgagcgctcc gaggaggatg
      961 ccgacgaggg cgaagacgag caggcgacgg cggttcacgc ctcgcaggct atcctcccga
     1021 ccaccgccgg ccgggtggaa ccgctcagcg ccggcgctgc acgcgggcct cgtcccagac
     1081 cggatcggcc gactcgtaga cgcgcccgtc ggcgccgaag acgaggaagc ggtcgaactg
     1141 ccgcgcgaac cagcggtcgt gcgtcaccgc gacgacggtg ccttcgaagg cgtccagcgc
     1201 ctcctccagc gcttccgccg agtgcaggtc gaggttgtcc gtcggctcgt ccaacagcag
     1261 catcgtcgcg ccggacaact cgagcaggag gatctggaag cgcgcctgct gtccaccgga
     1321 gagcatctcg aaggtccgct cccccgccat cgcgaggccg tagcggtcca gcgccttggc
     1381 cgcctcctcc cgcggcttgc ccgcgcggtg ctcgtcgccg cggtgcagga tctcgagcag
     1441 ggtgcgcccg tgcagcgccg aatggtcgtg agtctgggcg aaccagcccg gccgcacccg
     1501 ggatccgagc cgcgcgatcc cggtgtgcgc caccggtgtc ggagcgaact cccccacggg
     1561 ccggtgctcg atgtccgggt cgctgccgcc ggcggcgagc aggcgcaaga agtgcgactt
     1621 gcccgatcca ttggagccga gaatggcgat ccgctcgccg aaccacagct cggcgtcgaa
     1681 gggcttcatc agctgggtcc ccgagccgcc gtcgggtgac gcgatctgca actccagccg
     1741 ctcacagacg acggcgcgct tggcggtgcg cccgccggcc aaccgcatac ggacgttctg
     1801 ccgcaagggc accttctccg gcggaccggc ctcttcgaac ttcgcgagcc gggtcttggc
     1861 cgcctgatag cgcgaggcga ggccgtcgtt gtaggcggcc ttgaccttgt acatctgcac
     1921 gaggtccttg agcttctggt gctcctcgtc ccagcggcgc agcagttcat cgagccggtc
     1981 gttgcgttcc tggcgggctt ggtcgtatgt cgtgaacgac cccgggtgca cccagatcgt
     2041 cgcgccggcc gagttaggtt ccagcgtcgc gatgcgagtc gcgctctggg acagcagttc
     2101 tcggtcgtgg ctgatgaaga gcacggtctt gggcgaggtc cgcagtcgtt cctcgagcca
     2161 acgtttgccc ggcacgtcga gatagttgtc gggctcgtcg aggacgagca cttcctcggg
     2221 tccgcgcaac agtgcttcca gcacaaggcg tttctgctcg ccgcccgaga gcgtggtgac
     2281 cttgcgccac tgcgccttct cgaacggcat accgagcgcc gccatcgtgc actcgtccca
     2341 gagggtctcg atctcgtagc cgccgacatc gccccagtcc acgagcgcct gggcgtaggc
     2401 gagctgatcg gcctcggagt cgcgctccat catcagcagc tcggtgcggt cgatctcggc
     2461 ggcggccttg cgaatcggct ccgccgcggc gagcagcagc agatcgcgga ctgtggagtc
     2521 atcgcggacc tggccgatga actgccgcat gaccccgagg ccgccggagc gcacgacggt
     2581 gccgtcgtcg gggacgaggt cgccggcgag gatgcggatc agcgtggtct tcccggtgcc
     2641 attgggtccg atcagcgcgg tcttcgagcc ctcgccgacg cgcagattga catcgcgcag
     2701 cagcggccgc ccgtccggca gccagtactc cactccagcg atgtcgacgt gacccacgag
     2761 agtcaatgat gcggtgtgcc gcccgcctca cccaaccgat ttgcgccggg tcgtcattcg
     2821 cccgggtagg agtcgatatt ccagaggttg ccctcggcgt cgcgcacggc ggcgctgcgg
     2881 ccaccgtgcg gcggatggtg cggctcctgc gcgagcgtgg cgccgtggtc gagggcggcg
     2941 gtgagcacgc ggtcgacctc cgcgtcgctc gtcacgacga gattgcaggc gccgacaccg
     3001 ggccgcgacg cgaatccgag cgtgtcgtcg tggcggaccg acccgaacat cagcccgcca
     3061 tggtcgcgcc agcggaattg cgcgtgtacg acggtcgccg ggtcgtccgg gtcgcggacc
     3121 acgaccacct cggtgaagcc gatcgcggtg aggaagtcga tcgcacgatc gacatcggtg
     3181 aaggtcaggc agtggaagag ttgcggcttg tccatgcctc cagactgctg cacccagtga
     3241 gctgggtctt ggagaaatgg gaagtctctc gagatgccgg accggatatg cacatgttaa
     3301 tgtgcataga catgagcgct ctcgaacgac ggctccacct gcttctcgac caggaccgtc
     3361 acgatcggct cgtggcagaa gccgaacgca cgggccgatc cgtgaatgcg gtgattcgtg
     3421 ccgccatcga cgagcatttc gacgagcagc gtgaccaggt gcgccgcgcc gcggcagcac
     3481 ggctgttgct ggcaagcgcc gcggcaccgg gctccgagcc ggccgagacc tgggagcaga
     3541 tgctggccgc gcaggacgcc gacctccagg actcgctgcg cccgtgatcg acctctttgt
     3601 cgacagtccc gtattcctgc acgcgctggg cgcgccggtc gcggtgacgc cagcctgcca
     3661 ggcactgatc cgttcggctc aggagaacac agtgcgactg catacggcgg tcgaatgcgt
     3721 gcaggagatc gccttccatc gaacgcggat gggcgatgtc tcccgcgcgc tggctcaggt
     3781 caacgcgatc cgtgacctgt gcgtcgtcca ccccttcgac gaccgcgtcc tcgaccgcgg
     3841 ccttgagctc atggcggcga ccacgattcg cgggcgcgat gccttcatcg ccgcgacggc
     3901 gctgcttgcc ggcttcgagt cgatcgtcac gacggatgcg cgattcgtgg aggtgccggg
     3961 gctgcggcct gccgggcccg gcagctggac cgcctagttg gcgtcccgga cgtccgcggc
     4021 atacagggca aggatccggt ctgccgcttc catggctgac agtgacccgg cccgcaccgc
     4081 cgactccagg gccgcgcgct gctcgcgcac cgaatgggta tgccgcacag cgtcttccag
     4141 gtgcgcgtcc accatcgccc acatccagtc ctgctgctgt tgggcgcggc gagtggccag
     4201 cgatccctcg gcttcgaggt ggccgcggtg atcgaggacg gcctgccaca cctcgtcgag
     4261 cccggaatgc gttgcggcgg aacaggtcag caccggtggc cggcgaacat ccttgcccgg
     4321 caacatcaat cgcatcgcgg tggcgaggtc acgggcggca acgcgcgccc gtccttcccc
     4381 gtcgccatcg gccttgttga cggcgatgac gtcggcgagt tcgaggatgc cgcgcttgat
     4441 gccctgcaac tgatcgccgg cgccggccag ggtcaacagc aggaaggtgt cgaccatctc
     4501 cgagacggcg atctcgttct gccccacgcc gaccgtctcg acgatgatca cgtcataccc
     4561 ggcggcctcg agcaccagca tcgactcacg cgtcgcgcgc gccaccccgc ccaggtgccg
     4621 cccgctgggc gacggacgca caaacgcatg atcgctcgtg gtcagctcgg ccatgcgggt
     4681 gcggtcgccg aggatcgacc cgccggtgcg ccgcgacgac gggtcgaccg ccacgacacc
     4741 aacccggttc ccccgctgga tcagccgcgt cccgagcgca ttgatgaagg tcgacttgcc
     4801 cgcgccgggc acgccggtga tgcccacccg caccgaggag ccggtatgcg gcgtgagtgc
     4861 gccgatcaac tcgctcgcct tggaccggtg atcctcgcgc gacgattcga cgagcgtgat
     4921 cgcacgggcg atcgccgccc gggacccgga caggacgtcg gccgtcagcg cggcgacgtc
     4981 gatctcacgc atccaggcgg gcccgcaggt cgcggatgag gtcctgagcc gcggcggcga
     5041 tcaccgtgcc gggcggatag atggcgtccg cgccggcagc ccgcagttcc gcgaagtcct
     5101 ggctagggat gaccccgccg acgacgatca tcagatcctc gcgaccgagc ttgtccagct
     5161 cctggcgcag cgccggcacc agagtcaggt ggccggccgc gagcgaggag acaccgacga
     5221 tgtgcacatc ggcttcgacg gcctgccgcg caacctcttc cggcgtctgg aagagcgggc
     5281 ccacgtcgac gtcgaaaccg aggtcggcga acgcggtcgc gatcaccttc tggccgcgat
     5341 cgtgcccgtc ctgccccatc ttggcgacga ggatgcgcgg ccgccggccc tctgcctcct
     5401 cgaactcctc gaccagctgc tgggcagcgt gcacgccctc gtcgtcgccg gcttctgcgg
     5461 aatacacccc accgatcgta cggatcgtcg cggtgtagcg gccgaaggac ttctccatcg
     5521 cctccgagat ctcgcccacg gtcgccttcg cccgggcggc gtcgatggcc agtgccagga
     5581 gattcgacga cagatcgcgg ccgccgtcct tgctgcacgc ctcggtgatc ttcgccagcg
     5641 ccgcgtcggc ctcggcctgg tcacgctcgg cgcgcagccg ctcgagcttg gcgatctgct
     5701 gcgcccggac cgcggcattg tcgaccttga ggacgtcgat ctcctcgtcg tcctcgacga
     5761 catagcggtt gacgccgatg accggctgct gcccggaatc gatgcgcgcc tgggtgcgcg
     5821 ccgccgcctc ctcgatgcgc atcttcggga tcccggcctc gatggccttc gccatgccgc
     5881 cggccgcctc gacctcttcg atgtgctccc acgcgcggcg cgccagatcg tgggtgagtt
     5941 tctcgacgta tgccgatccg gcccacgggt cgatggtgcg gcaggtgccg gactcctgct
     6001 ggatgaccag ctgggtgttg cgggcgatgc gcgcgctgaa atccgttggg agagcgatgg
     6061 cttcgtcaag ggcgttggta tgcagtgact gcgtgtgccc ctgcgtgctc gccatcgcct
     6121 cgatcccggt gcgcacgaca ttgttgaaga cgtcctgcgc cgtcagcgac cagccggacg
     6181 tctgggagtg ggtgcgcagc gagagcgact tgtcgctctt cgggttgaac tgcttcacca
     6241 gcttggccca cagcaggcgc gccgcccgca tcttcgcgac ttccatgaag aagttcatgc
     6301 cgatcgccca gaagaacgac aggcgcggcg cgaactggtc gaccgtcagc ccggcgccga
     6361 gaccggcccg cagatattcg acgccgtcgg ccagcgtata ggcgagttcg aggtcctgcg
     6421 tcgcgccggc ttcctgcatg tgatagccgg aaatcgagat cgagttgaac ttcggcatgt
     6481 gctgcga
//
LOCUS       DAOPJG010000184         6479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_12707_length_6479_cov_7.390100, whole genome shotgun sequence.
ACCESSION   DAOPJG010000184 DAOPJG010000000
VERSION     DAOPJG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12707_length_6479_cov_7.390100"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..280)
                     /locus_tag="PKU75_13895"
     CDS             complement(83..280)
                     /locus_tag="PKU75_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPF82018.1"
                     /translation="MTWHGTYDLVVAGTGAAAFAAAVTAADEGMTVLMLESTDRWGGS
                     TAMSGGGLAAQQPSDATGSGW"
     gene            complement(388..645)
                     /locus_tag="PKU75_13900"
     CDS             complement(388..645)
                     /locus_tag="PKU75_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82019.1"
                     /translation="MSWADGAVCVESTFAVDVDWARDGMILTPSTVLTGRPAVVAEEP
                     DPPQLIYPHEASGRCGRRLVRTTPPRPPTSSARREAAQLLG"
     gene            complement(748..1005)
                     /locus_tag="PKU75_13905"
     CDS             complement(748..1005)
                     /locus_tag="PKU75_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82020.1"
                     /translation="MSGIEEVADAIANTPESIVDYHVDICFRDRPVRPHVQVTIGTPS
                     EYDAWRRPMPHAVAALLERGPRHAAEQAAAAVRVWFDTIRR"
     gene            1601..2035
                     /locus_tag="PKU75_13910"
     CDS             1601..2035
                     /locus_tag="PKU75_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82021.1"
                     /translation="MGAGTALVVWYAGQVHLVGHDETLARWLQSHFTRHFPEVEGLAW
                     PEPIYHPASVGIDIDLATGMRAHGGGFDIRMSPVLDRRAFATDDVALGDVVHSLSLVF
                     APCGSGSIRMRGRMLPGELQRAGTPTRPWTSAFVSEAEVWRA"
     gene            complement(2093..2431)
                     /locus_tag="PKU75_13915"
     CDS             complement(2093..2431)
                     /locus_tag="PKU75_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPF82022.1"
                     /translation="MDKPREVSLDDLEAARAGGEVALVDCREPAEYRAGHVPGALLIP
                     LGQLVDKRDEIPADGPVYVICQSGNRSLSGADLLRQMGIEAYSVAGGTGGWAASGREI
                     VAGPYPTAQG"
     gene            complement(2441..2794)
                     /gene="trxA"
                     /locus_tag="PKU75_13920"
                     /pseudo
     CDS             complement(2441..2794)
                     /gene="trxA"
                     /locus_tag="PKU75_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            complement(3618..3758)
                     /locus_tag="PKU75_13925"
     CDS             complement(3618..3758)
                     /locus_tag="PKU75_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82023.1"
                     /translation="MNFTGTVTANFMMNSTLGLLIVSQQLLLIGGGMKPLAAGALTLG
                     YR"
     gene            complement(3755..4444)
                     /locus_tag="PKU75_13930"
     CDS             complement(3755..4444)
                     /locus_tag="PKU75_13930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF82024.1"
                     /translation="MNDPKATTVTGGSFRGTDTLLAGLVLSIVSYWLFAVSLGTVAPA
                     VLQALNGPYADPAAKTWAGPLLITGRAIQGLSAACVMPATIAMVKAYWDGADRQRAVS
                     MCSIGSFGGAGLAAFLGGLIATTMGWRWIFVFSIVVSLVASLLVWGTPESRVGADPGH
                     RRFDIAGLAIFLVMLLALMMLLTFGGKTFGWLSGTGLALLAVALVGLAVFIAVERGRQ
                     GHSSTSASSPI"
     gene            4534..5196
                     /locus_tag="PKU75_13935"
     CDS             4534..5196
                     /locus_tag="PKU75_13935"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82025.1"
                     /translation="MGSDATDLLPRLSRISTTSLVDAAPALRILPPGLRPLVPGRPFA
                     GVVRTARADRDLMSVIGALQVCGAGDVLVVDAGGDERAVAGELFATEAQRRGLAGFVI
                     HGRSRDTATLATLSIPVWSTGVAPNAFPAKAIPQVDIPLDLSGVAVAPGDLIVGDDDG
                     LVVGTAAELAAALGGAETIEARERRLQSAMLAGASLFDAMNFDEHLAELRAGRPGGLA
                     LG"
     gene            5193..6383
                     /locus_tag="PKU75_13940"
     CDS             5193..6383
                     /locus_tag="PKU75_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPF82026.1"
                     /translation="MTAFARRARLVQQSAIREFLALAGDPAITSFAGGYPDPTLFPVD
                     ELHKIFDELLTPANAAALQYTASDGIPLLREQVAARLTRDGMPCTPDDILITQGGQQG
                     LELTAKLFLDDGDVVITERPTFLGALIAFNPYEPQYVSVPLDEHGLDVEALEEVLRTT
                     RRVKFVYTVPDYQNPTGRSLSLERRRRLVELAEEFDVMVLEDTPYRELRYEGERLPAI
                     KSLDRTGRVIHVGSFSKILVPGFRLGWVLATPEIREKLVLLKLGADTQNGTLNMHAAA
                     AYLAGFDIEEHIRAMLPVYRHKRDLMLATMAEHFPEGITWTHAQGGLFTWVTFPESLD
                     AAAFQRDVLIPRAGVIIVPGAPFFTEDPAPAHHARMSFSGVPDDRLVAGITAMGALLR
                     EALR"
BASE COUNT          990 a         2217 c         2286 g          986 t
ORIGIN      
        1 ggcttccttg cgatgccggc tcgcgcccgg gccggcatcg ccgacggttg cgtcgaggta
       61 cgtcagcgcc tcctcccgtg agtcaccagc ccgatcccgt tgcatcagag ggttgttggg
      121 cagccagccc gccgcccgac atggctgtgc tgccacccca acgatcggtg ctctcgagca
      181 tgaggacggt cataccctcg tcggccgccg tgacggccgc ggcgaaggcg gccgcgccgg
      241 tgccggcgac gacgaggtcg taggtgccat gccaggtcac ggacctgatg gggctccact
      301 ccttgggtgc gcggcgcctc gcgccgtcac cgacactccg ccagggcgga ccggtcggct
      361 gccggcacat cgctcatgga ctgagcctca cccgagcaac tgggcggcct cgcgtcgtgc
      421 cgaggaggtc ggcggtcgcg gaggtgtggt ccgcacaaga cgtcgaccac aacgccccga
      481 cgcctcgtgc ggatagatca actgcggcgg atcgggctcc tcggcgacga cggccgggcg
      541 gccggtgagc acggtggacg gcgtgaggat catgccgtcg cgggcccagt cgacatcgac
      601 cgcgaaggtg gactcgacgc agacggcacc gtcggcccag gacacccctc gccgagatcc
      661 gcgaccagac ccgcgccccc ccgggtcgcc aattgctctg cgcgataagc catgtcggct
      721 tgcaggacgc cgagcgtggc cggccactca tcggcgaatc gtgtcgaacc acacgcgcac
      781 agcggccgcg gcctgctcgg cggcatggcg tgggccacgc tcgagcaggg cggcgacagc
      841 gtggggcatc ggccgccgcc aggcgtcgta ctcgctgggg gtgccgatgg tcacctgcac
      901 gtgcgggcgc acgggccggt cccgaaagca gatgtccacg tgatagtcga cgatcgactc
      961 cggcgtgttc gcgatcgcgt cggcgacctc ctcgatcccc gacaccgggt gctcgcagac
     1021 gggcgacagg aagtcgggga tagggatggg caggatcgat cagcgcctca agcagagcac
     1081 gctccggatg cgggcaccgg ccggcggcgg ccagccgcgc tcgcacgtgc gggtaggaat
     1141 ggctgcgacc gcgcaggtgg agcgtctcgg tgatctccgg ccccggtgac agggcgaacc
     1201 gcgagcgcgc gaggctgtcg ctcgtcagtg gaatccggaa caccgtctca gggtgccact
     1261 ggcccggtcc gggtgggccg gattcgttcc tgttcgaatt gcttccccac gcccggggca
     1321 cgcgagtgga ctgccgccgt cagcatgtcc atgtgcaggg aaggaaggac gtcatgcagt
     1381 cacggacaga tggattgggt cggcgcgggt tgctgcaggg tgcggcgggg gctgggctcc
     1441 tcgccgtagg cggactggtc gcgcccagca ccgcacgcgc ggcggtgacc gaccccgcga
     1501 cccgcaaggt catcctcgtg ggcgccaacc ccggcatcca gcgcttcgac gtcgagacct
     1561 gcacccctat gcgtcgtgct ggcgggtcga ctggtcggtc atgggagccg gcaccgccct
     1621 ggtggtctgg tatgccgggc aggtgcacct ggtcgggcac gacgagacgc ttgcgcgctg
     1681 gctgcagagc catttcaccc gtcacttccc cgaggtcgag ggccttgcgt ggccggaacc
     1741 gatctatcat ccggcgagcg tcggtatcga catcgatctc gcgaccggca tgcgtgctca
     1801 tggcggcggt ttcgacatcc gcatgagtcc ggtcctcgat cgtcgtgcct tcgccacgga
     1861 cgacgtcgcg ctgggggacg tcgtgcacag tctcagcctg gtcttcgccc cgtgcggcag
     1921 cggttcgatc cggatgcggg gccggatgct gccgggtgaa ctccagaggg cgggcacgcc
     1981 cacgcggccg tggacgtccg ccttcgtcag cgaggccgag gtctggcgcg cataggaccg
     2041 tcgcgccggg tgaccggccc gcctcggcgg actgcactat cgacgaggcg ggtcagccct
     2101 gggccgtggg gtagggcccg gcgacgatct cgcggccgga cgcggcccag ccgccggtgc
     2161 caccggccac cgagtaggcc tcgatgccca tctgccgcaa cagatccgcg ccgctgaggc
     2221 tgcggttgcc cgattggcag atgacgtaga cgggcccgtc cgcggggatc tcgtcgcgct
     2281 tgtccacgag ttgccccagc gggatcagca gcgccccggg cacgtgcccg gcgcggtatt
     2341 cggcgggctc gcggcagtcg acgagcgcca cctcgccgcc tgcccgcgcg gcctcgaggt
     2401 cgtcgaggga gacctcgcgc ggcttgtcca tcaggtcgcg ctgggcgctg acctgggcgt
     2461 gcacggcagc catgtcgagg cccttgacac cctcgatgac ctgctcgagg ccggcggcgg
     2521 ggagggcccc gggctgggag aagacgagga tgccctcgcg gaaggccatc agcgtgggga
     2581 tcgaggtgat attcgccgcg cccgcgatgc ccggctgcgc ctcggtgtcg agcttgccga
     2641 acacgatgtc cggatgtttg tcggacgccg cctcgtagac gggcgcgaat tggcggcacg
     2701 ggccgcacca ggctgcccag aagtcgacga gcacgatgtc gttgctcgag acgacctgct
     2761 cgaagtcggc gtcggtgagt tcccgggtgg tcatcgtcgt cctcactgtg ttaggtctcg
     2821 tgtcgcgtcc cactataccc catggggtat gccgcccggt ccgcttcgcg actgaggcta
     2881 gcccggtcag gccgccgggt cgagcgtcga tcggggacgg ccggccttgg tcagcgcgac
     2941 tcgaggaaag cgctcaccgc cgccacggcc gcggccgtcc cggtggccag ggtcggttgc
     3001 actaccggcc cgaaaccggg attgtggttc ggcactggcg tcgtgccctc gacgaatcca
     3061 ccgaatcccc aatagcagta aggaattccg aaggcgtcgg ggatgacgct gaaatcctcc
     3121 taggccggga tcggggccag ctgcgtgact tggtcgactc cgaattcggc caccagggcc
     3181 gatcgcacgc ggtcggtcac cacggggtcg ttgtcggtca gggggtagac gtcatagggc
     3241 tcgatctcgg gttcgcgagg cgagccggct gcctcgcact ccgcacggat gatccgttcg
     3301 atcgccgcca ggagctattc gcgcaccgcg tcgtcgtagg cgcggaggcc atcttgaaga
     3361 tccccatccc ggcgccggcg cgctcggccg gcagactggt caacgcggcg tcggtcgccg
     3421 gcgtggcgaa gaaggcgaga cccagcccga agcaggcgta tgccgtcacc gcgagccatt
     3481 tgtattgtga gagtaagagg ttggtcggca tgagcaaggc gccgcaaggg ccaccaacag
     3541 cgcgccccag atcatcggct tgcgcggccc atcgcggcgc agcaatcgct cgcccgcgcg
     3601 aatgaagagg acgaccatca gcggtagccc agggtcagtg ccccggcggc cagtggcttc
     3661 atgccaccgc cgatgaggag cagttgctgg gagacgatga gcagtccgag cgtcgaattc
     3721 atcatgaagt tcgccgtgac ggtgccggtg aagttcatat tggagaagag gccgaagtcg
     3781 atgaatgccc ctgccggccg cgctcgacgg cgatgaagac cgccaacccc accagcgcca
     3841 ccgccaatag cgcaaggccc gtcccggaca gccagccgaa ggtcttgccg ccgaaggtca
     3901 gcagcatcat caacgccagc agcatgacga ggaagatggc gaggccggcg atgtcgaagc
     3961 gtcggtgccc ggggtctgcg ccgaccctgc tctcgggtgt gccccagacg agcaggctcg
     4021 ccacgaggga gacgacgatc gagaagacga agatccaccg ccagcccatc gtggtcgcga
     4081 tgagtccgcc gaggaaggcc gcgaggccgg ccccgccgaa tgagccgatg gaacacatcg
     4141 acaccgcccg ctgccggtcc gccccgtccc aataggcctt gaccatcgcg atggtcgcag
     4201 gcatgacaca cgcggccgag aggccttgga tcgcccgacc ggtgatgagg agcgggccgg
     4261 cccacgtctt cgcggcggga tcggcatacg gtccgttgag ggcctggagc acggcggggg
     4321 cgacggtgcc gagcgagacg gcgaagagcc agtaggacac gatgctcagg accagcccgg
     4381 ccagcagcgt gtcggtgcca cggaatgatc cgccggtgac cgtggtggct ttcgggtcgt
     4441 tcatgtcggt cccttgccgc gaactcgtct ttcgatgata gggggggacg gaacccggct
     4501 gcacagggcc ctgctcgcga ttacggtggg cgcatgggga gcgatgccac cgatctgttg
     4561 cctcggctga gccggatttc cacgacaagc ctcgtggacg ccgcaccggc gctgcggatc
     4621 ctgccgcccg ggttgcgccc gctcgtgccg ggccgtccgt tcgcgggcgt ggtgcgcacc
     4681 gcccgggccg atcgagacct catgtcggtg atcggtgcgt tgcaggtatg cggcgccggc
     4741 gatgtgctcg tggtcgacgc cggaggcgat gagcgcgctg tcgcgggcga gctgttcgcc
     4801 accgaggcgc agcgacgtgg gctggccggg ttcgtcatcc acggacgcag ccgtgacacg
     4861 gcgacgctcg cgacgctgag catcccggtc tggtctaccg gcgtggcgcc gaatgccttc
     4921 cccgccaagg cgatcccgca ggtcgatatc ccccttgacc tgagcggcgt cgcggtggcg
     4981 cccggtgatc tgatcgtcgg cgacgacgac ggactggtcg tcgggacggc ggctgagctg
     5041 gccgccgccc tcggcggagc ggagacgatc gaggcgcggg aacggcggct gcagtccgcg
     5101 atgctggccg gcgcttctct tttcgacgcg atgaacttcg atgagcatct cgccgagttg
     5161 cgggccggac ggccgggcgg attggctctc ggatgaccgc cttcgcccgc cgcgcccgct
     5221 tggtgcagca gtccgcgatc cgcgaattcc tcgctctcgc aggggatccg gcgatcacgt
     5281 cgttcgcggg cggttatccg gacccgaccc tgttccccgt cgacgagttg cacaagatct
     5341 tcgacgaact gctgactccg gcgaacgccg cggcgctgca gtacaccgcc tccgacggca
     5401 taccgctgct gcgtgagcag gtcgcggccc gcctgacgcg agacggtatg ccgtgcaccc
     5461 ccgacgacat cctgatcacc cagggcgggc agcagggcct ggaactgacg gccaaactct
     5521 tcctggacga cggcgatgtc gtgatcaccg agcggcccac cttcctcggc gcgctcatcg
     5581 ccttcaaccc ctatgagccg cagtatgtct cggtgccgct ggacgagcac ggcctcgacg
     5641 tcgaagcgct ggaagaggtg ctgcggacga cccgccgggt caagttcgtc tacacggttc
     5701 ccgactatca gaacccgacc ggccgcagcc tcagcctcga gcggcggcgc cggctcgtcg
     5761 aactcgccga agagttcgac gtcatggtcc tcgaggacac gccgtatcgc gagctgcgct
     5821 acgagggcga gcggctgccg gcgatcaaga gcctcgaccg aaccggccgg gtcatccatg
     5881 tcggcagctt ctccaagatc ctggtgcccg gcttccggct cggctgggtg ctcgccacgc
     5941 cggagatccg cgagaagctc gtgctgctca agctcggcgc ggacacgcag aacggcaccc
     6001 tcaatatgca cgcggccgcg gcctacctcg ccggcttcga catcgaagaa cacatccgag
     6061 cgatgctgcc ggtctaccgg cacaagcggg acctgatgct cgccaccatg gccgagcact
     6121 tccccgaggg aatcacgtgg acccacgcgc agggtggtct cttcacgtgg gtcaccttcc
     6181 ccgagtccct cgacgccgcc gccttccaac gcgacgtcct catcccacgg gccggagtca
     6241 ttatcgttcc cggcgcgccc ttcttcaccg aggacccagc cccagcccac cacgcccgga
     6301 tgagcttctc cggtgtcccc gacgaccggc tggtcgccgg gatcactgcc atgggagccc
     6361 tgctgcgaga agcgctccgc tgagccggct cgtccaccag cccgggtcgg tccacggcag
     6421 gagggcgtcg cggcgggcga tggcggcggc ttcgccgcgt gttgcggcgc cgagcttgg
//
LOCUS       DAOPJG010000185         6359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_13073_length_6359_cov_7.167354, whole genome shotgun sequence.
ACCESSION   DAOPJG010000185 DAOPJG010000000
VERSION     DAOPJG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13073_length_6359_cov_7.167354"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKU75_13945"
     CDS             complement(<1..574)
                     /locus_tag="PKU75_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF82027.1"
                     /translation="MASTTTRLRGLVWVVPALALLGIFVYYPIVENIRLSFYSWNAFS
                     PNPVFVGLQNYTDAAADPIFWRSLRNNIAYAVVSLICQVGFSLCLAAVLEEFVHQKLR
                     GVLRVIYFIPAVISITVSGILFSFIYNPQFGLLNRLLGAMGLERFQHAWLGEQKTAIW
                     SIIGMSQWQSIGYTAVLFVVEGRRSDGCIGA"
     gene            complement(668..2011)
                     /locus_tag="PKU75_13950"
     CDS             complement(668..2011)
                     /locus_tag="PKU75_13950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPF82028.1"
                     /translation="MTHRTRTTALIAVTAAGALAACGGPGTATDKTSAAPQLTASATT
                     KEGTLSIITKFADPKYAPYFEKVATAYQSANPGVKVDLQQVGDQPFKDKIRVLSASKQ
                     LPDIYFSWAGDFANKFVRAGLAADLTSVIGPDTDWGKTFSPAALKAFQYDGKSYGVPI
                     NLDAKYMAYNTAIFDKVGVTKPATLEDLLSTCGTLKAAGYTPIAFGNQYGWPAIHFMT
                     QLNAMDVPEATRSTDYNPATGAFTDGGYAKALEQFKQINDACFESGSNGLSHETAQAN
                     FLSGKAAMHFLESVEFSGLTEKGGAPADLANNWDFFRLPPAGDAAGDKGALTGAPDGF
                     MVNSASKNAALAVDFLKFMTSKENGATMTKDIGWLSSVQGTATAENSFPQLETALKDI
                     SSANSFAIWLDTVTNAEVANAYLSGVQGILDGSQTPADVMKGVQSAAEKAKKSVG"
     gene            complement(2023..2886)
                     /locus_tag="PKU75_13955"
     CDS             complement(2023..2886)
                     /locus_tag="PKU75_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPF82029.1"
                     /translation="MAPRLLCLGDNVVDRYVDTGVMYPGGNALNVAVHARRLGVDAAY
                     LGVTGDDAAGDLVRSALISERVDVARVKTRPGMTAYADVRLIEGNRVFGHGDPGVSRF
                     VLGPDELADVGAFDLVHTGECSGVEDQLPAIAQAAARLSFDFSERPWEYIAQFAPIAD
                     IAIASLPSGEHGTAVDYGLRIAALGPRIVVVTGGPQGAWLISDGQVAHAAAGTGPIVD
                     TLGAGDAFIARFLTGLLREEPSAHALAAATAYATDACATFGAFGHPGAPIETPAPGVE
                     HPAWAQQQPRN"
     gene            complement(2888..3934)
                     /locus_tag="PKU75_13960"
     CDS             complement(2888..3934)
                     /locus_tag="PKU75_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPF82030.1"
                     /translation="MTTAATQPDGLLNFDAERYVRIQSGAVARGRELGSALAQRYAAG
                     RVEEVVLLGTGGAGILMEPALRLAESRLSVPVRLRHPAEELAAGGPGLGAGSAVVIPS
                     LSGTTKESVAAQELAHRHGAWTAVLTAHGDSPLAQAADLTLVNEAADDTSSESFYLQS
                     ILGIAGLAAETDDWGAAGSLGAHLESVPQHLLTAKTAYEDRAEQAAHFLAERDYHIIT
                     GAGYAWPEAHYYGMCILEEMQWIRTRPVHAAHFFHGTLELLEAGSSLVVLVGRDETRE
                     LTDRVLDFARTITEHILVIDTADADLGQLPPDVSAAMAPVVAAALLERVSAHLEVLRD
                     HPLTTRRYYRRIDY"
     gene            complement(3934..4932)
                     /locus_tag="PKU75_13965"
     CDS             complement(3934..4932)
                     /locus_tag="PKU75_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPF82031.1"
                     /translation="MTQVLDLKPIDADTEEKLSGAIAERDRIREVVAKAVEDGLTNIW
                     FVGAGGSLICSYPAHYVLQQHASVPTFQLQSDELNCSIPKLMGKGSLVVLASYTGKTK
                     ETVEAAKTASGTGATVIAGAKEGSPLAEAVSIAFSGKSDIFELLVALAVLEATGADLP
                     WDEIETGLAALPAAVKAAVEEADADLASIAEDFKDDPVTYVLGSGPSYGWAYGMAMCF
                     LQEMQWKHAAGFNSGEFFQGAFEVIDNDSAVILWLGEDASRPMGERAQAFLKTYAARA
                     RTIDVAALELPGVPASVRPIVSPLVVGALANRLAQRYEHTRNHSLENRHYMFKVDY"
     gene            5118..6200
                     /locus_tag="PKU75_13970"
     CDS             5118..6200
                     /locus_tag="PKU75_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF82032.1"
                     /translation="MASEVRSRVTVIDVARAAGVSVATAARALGGYGSVSQASRRKVD
                     EAAARLGYTPNMVARSMITGSTKTIGVIVADIENQFFQLVLRGVSRVLSEAGFDLVLA
                     NTEESIEVERRSLRVMTSRRVDGLILSPTEVADVAQIRAVADSGIPVVLLDRRLRGLK
                     VDSVGIRNRAASHEATTYLLDRGHRQIAVVTGGGAEHAEELRSGNVGRLRRMSATTLG
                     TRTAGYLEAIEAAGIAHVPDYVVANGFRQSDARTAARELMRLPQPPTAIMAFDSLLNL
                     GVLQGLGDAGVRCPDQVSLIGFDDAPWAEVVDPPLTVIAQPTEEIGALAARQLLDRLG
                     GESGAPMNHRLPVRLIERQSVARPPG"
BASE COUNT          951 a         2040 c         2298 g         1070 t
ORIGIN      
        1 aggcgccgat gcacccgtcg ctgcgacgac cttcgacgac gaagagcacg gccgtatagc
       61 cgatggactg ccactggctc atcccgatga tgctccagat cgcggtcttc tgctccccga
      121 gccaggcgtg ctggaaacgc tccagcccca tcgcgccgag cagccggttg agcagcccga
      181 actgcgggtt gtagatgaag gagaagagga tgccggacac ggtgatggag atgaccgcgg
      241 ggatgaaata gatgacccgc aggaccccgc gcagcttctg gtgcacgaac tcctcgagaa
      301 cggcggccag gcacagcgag aagccgacct gacagatcag cgacaccacg gcatacgcga
      361 tgttgttgcg aagcgagcgc cagaagatcg ggtcggccgc cgcgtcggtg tagttctgca
      421 acccgacgaa gaccggattg ggggagaagg cgttccagct gtagaagctg agccggatgt
      481 tctcgacgat ggggtagtag acgaatatcc ccaacagggc cagcgcgggg acgacccaca
      541 ccaggccacg tagtcgcgtc gtcgttgacg ccatgcgttg atcctccttc gcccgggggc
      601 gatatggcgg atcggggggc ggccggctat tgcggcgcgg ccaccccccg acgagggggt
      661 atgccggtca gccgaccgac ttcttggcct tctcggccgc gctctggacg cccttcatga
      721 cgtccgccgg ggtctgggat ccgtcgagga tgccctggac gccggagaga taggcgttgg
      781 cgacctcggc gttggtgacg gtgtccagcc agatcgcgaa gctgttcgcg gagctgatgt
      841 ccttcagcgc cgtctcgagc tgcgggaagg agttctcggc ggtcgcggtg ccctggaccg
      901 agctgagcca gccgatgtcc ttggtcatcg tggcgccgtt ctccttgctc gtcatgaact
      961 tcaggaagtc cacggcgagg gcggcgttct tgctggcgga gttgaccatg aacccgtcgg
     1021 gtgcgccggt gagggcgccc ttgtcgcccg cggcgtcacc ggccggcggc agccggaaga
     1081 agtcccagtt gttcgccagg tccgccgggg cgccgccctt ctcggtcagc ccggagaact
     1141 ccaccgactc caggaagtgc atggcggcct tgcccgagag gaagttcgcc tgcgcggtct
     1201 cgtgcgagag gccgttgctt ccggattcga agcaggcgtc gttgatctgt ttgaactgct
     1261 cgagcgcctt ggcgtagccg ccgtcggtga atgcgccggt cgccgggttg tagtcggtgg
     1321 agcgtgtcgc ctcggggacg tccatcgcgt tgagctgggt catgaagtgg atcgccggcc
     1381 agccgtactg gttgccgaag gcgatcgggg tgtagcccgc cgccttcagc gtgccgcagg
     1441 tgctgagcag gtcttcgagc gtggccggct tggtgacccc gaccttgtcg aagatcgccg
     1501 tgttgtaggc catgtatttc gcgtcgaggt tgatcggcac gccataggac ttgccgtcat
     1561 attggaacgc cttgagcgcc gcgggcgaga aggtcttgcc ccagtcggtg tccggcccga
     1621 tcacggaggt caggtcggcg gccaggccgg cgcgcacgaa cttgttcgcg aagtccccgg
     1681 cccaggagaa gtagatgtcg ggcagctgct tggaggcgga caggacgcgg atcttgtcct
     1741 tgaatggctg gtccccgacc tgctgcaggt cgaccttcac gcccgggttg gcgctttggt
     1801 atgccgtggc caccttctcg aagtagggag cgtatttcgg gtcggcgaac ttggtgatga
     1861 tgctgagcgt gccctccttc gtggtcgcgg aggccgtcag ttgtggggcg gccgacgtct
     1921 tgtcggtcgc cgtcccgggg ccaccgcacg cggcgagtgc gccggcggcg gtgacggcga
     1981 tgagggcagt ggtgcgggtg cggtgcgtca tggtttcctc cgtcagttgc ggggctgttg
     2041 ctgggcccac gccgggtgct ccacgcccgg cgcgggggtc tcgatgggag cgcccggatg
     2101 gccgaacgct ccgaaggtgg cgcacgcgtc ggtcgcgtat gccgtggccg cggcgagcgc
     2161 gtgcgcggac ggctcctccc gcagcagacc ggtcaggaag cgggcgatga acgcgtcgcc
     2221 cgcgccgagt gtgtcgacga tcggcccggt gccggcggcc gcgtgcgcga cctgcccgtc
     2281 gctgatcagc catgcaccct gcgggccccc ggtgacgacg acgatccgtg gccccagtgc
     2341 ggcgatccgc aggccgtagt cgacagccgt cccgtgctca cccgagggca gcgatgcgat
     2401 ggcgatatcg gcgatggggg cgaattgcgc gatgtactcc cacggccgct cggagaagtc
     2461 gaaggagagg cgcgccgccg cctgcgcgat ggcgggcagt tgatcctcga cgccggagca
     2521 ctcgccggtg tgcaccaggt cgaatgcgcc gacatccgcg agctcgtccg gcccgaggac
     2581 gaaccgggac accccgggat cgccgtgccc gaagacccgg ttaccctcga tgagtcgcac
     2641 gtccgcgtat gccgtcatac cgggccgggt cttcacgcgt gccacgtcga cccgctctga
     2701 gatgagtgcg gagcgcacga ggtccccggc cgcgtcgtcc ccggtgaccc cgaggtatgc
     2761 cgcatcgacg ccgagccggc gcgcgtgcac cgcgacattg agcgcgttcc cgccgggata
     2821 catcacgccg gtgtcgacat agcggtcgac gacgttgtca ccgaggcaga gcagccgggg
     2881 agccacgtca gtaatcgatc cggcggtagt agcggcgcgt cgtgagcggg tggtcgcgca
     2941 ggacctcgag gtgcgcgctg acacgttcca gcagcgccgc ggcgaccacg ggtgccatgg
     3001 ccgccgagac gtccgggggc agctgcccca gatcggcatc cgcggtgtcg atcaccagga
     3061 tgtgttcggt gatcgtgcgc gcgaagtcga ggacccggtc ggtcagctcc ctggtctcgt
     3121 cgcggccgac gaggaccacg agtgaggagc cggcctcgag cagttcgagg gtgccgtgga
     3181 agaagtgcgc cgcgtggacc ggccgggtgc ggatccactg catctcctcg aggatgcaca
     3241 tcccgtagta gtgcgcctcg ggccaggcgt atccggcccc ggtgatgatg tggtagtcgc
     3301 gctcggcgag gaaatgcgct gcctgctcgg cccggtcttc gtatgccgtc ttggccgtca
     3361 ggaggtgctg cggaaccgat tcgaggtggg cgcccaggga gccggcggcg ccccagtcgt
     3421 cggtttccgc cgccagcccg gcaatgccca ggatcgactg cagatagaag gactcgctcg
     3481 aggtgtcgtc ggcggcctcg ttgaccaggg tgaggtcggc agcctgtgcc aggggcgagt
     3541 ccccgtgtgc ggtgagcacg gcggtccagg cgccgtggcg gtgtgccagc tcctgcgccg
     3601 ccacgctttc cttggtcgtc cccgagagcg aggggatcac gaccgcgctt ccggctccca
     3661 gcccgggacc gcccgcagcc aactcctccg cggggtgccg cagccgaacg ggaaccgaca
     3721 accggctctc ggccaaccgc agggcaggct ccatgagaat gccggcgccg ccggtgccga
     3781 ggaggacgac ctcctcgacc cgtcccgcgg cataccgctg cgcaagcgcg cttccgagct
     3841 cccgtccgcg cgctaccgcg ccggactgga tacgcacata gcgctccgcg tcgaagttga
     3901 gcagcccgtc gggctgcgtc gcggcggtcg tcatcagtag tcgaccttga acatgtagtg
     3961 gcggttctcc aacgagtggt tgcgcgtgtg ctcgtagcgc tgcgccagcc ggttggccag
     4021 ggcaccgacg acgagggggg acacgatcgg tcgaacgctc gcgggaacgc cgggcagctc
     4081 gagggcggcc acgtcgatcg tccgggcccg cgcggcatat gtcttcagga aggcctgggc
     4141 tcgctcgccc atggggcgag aggcgtcctc gcccagccac aggatgacgg cggagtcgtt
     4201 gtcgatgacc tcgaaggcgc cctggaagaa ctcgccgctg ttgaagccgg cggcgtgctt
     4261 ccactgcatc tcctgcagga agcacatcgc catcccatag gcccagccgt agctaggacc
     4321 ggagccgagc acataggtca ccgggtcgtc cttgaagtcc tcggcgatgg aggcgaggtc
     4381 ggcgtcggcc tcttcgacgg ctgccttgac ggctgccggc agggcggcga gaccggtctc
     4441 gatctcgtcc cagggcaggt cggcgccggt cgcctcgaga acagccaggg cgacgaggag
     4501 ttcgaagatg tcgctcttgc cggagaacgc gatggagacg gcctcggcga gcgggctgcc
     4561 ctccttggcg ccggcgatga cggtggcgcc ggtgccggac gccgtcttgg cggcctcaac
     4621 agtctccttc gtcttgccgg tgtaggaggc gagcacgacc agcgatccct tgcccatcag
     4681 cttggggatg ctgcagttga gttcgtcgct ctggagctgg aaggtcggga ccgaggcgtg
     4741 ctgctgcaag acgtagtggg ccgggtagct gcagatcagg gagccgccgg cgccgacgaa
     4801 ccagatattc gtcaggccgt cctcgaccgc cttcgcgacc acctcgcgga tccggtcgcg
     4861 ctcggcgatg gcgccgctga gcttctcctc ggtgtcggca tcaatgggct tcaggtcaag
     4921 cacctgggtc atggggttgt ccttcgtcgt gtaaccgatg tgtggacccg gcacggccgg
     4981 gggagcgttt gtggggcgca ctgataacgt tctcaatgag ccgatgcgat aacattctca
     5041 ccttcgtcga tcggctgtca atacctcgag aaggggaaag ttcgcggggt ttccggcgcc
     5101 gggatagggt cgcgagcatg gcgtcggagg tccgttcgcg tgtgacggtg atcgacgtcg
     5161 cccgagccgc tggggtgtcc gtggccaccg ccgcccgggc gctcgggggc tatggctcgg
     5221 tgagccaggc gagccgccgc aaggtcgatg aagccgccgc acgcctgggg tacaccccga
     5281 acatggtggc gcgcagcatg atcaccgggt cgaccaagac gatcggcgtg atcgtcgccg
     5341 acatcgagaa ccagttcttt caattggtgc tgcgcggtgt cagccgagtg ctctccgagg
     5401 ctggcttcga cctcgtcctg gccaacaccg aggagagcat cgaggtcgag cgccgctccc
     5461 tgcgggttat gacctcccgc cgggtcgacg ggctgatcct ctcgcccacc gaggtggcgg
     5521 acgtcgccca gatccgtgcc gtggcggact ccggcatacc cgttgtcctg ctggatcggc
     5581 ggctgcgtgg gctcaaggtc gactccgtcg ggatccgcaa ccgcgcggcc tcgcacgagg
     5641 cgacgaccta tctgctcgat cgcgggcatc ggcagatcgc ggtggtcacg ggcggtgggg
     5701 ccgagcacgc ggaggagctg cggtccggga acgtggggcg gttgcggcgg atgtcggcga
     5761 ccacccttgg gacacgaacc gccggatatc tcgaggccat cgaggccgcc ggcatcgcgc
     5821 atgtcccgga ctacgtcgtc gccaacggtt tccggcagag tgacgcgcgc acggccgctc
     5881 gagagttgat gcggctcccc cagcccccga cagcgatcat ggccttcgac agcctgctga
     5941 acctgggtgt cctccagggg ctaggtgatg ccggcgtccg atgccctgac caggtctcgc
     6001 tcatcggatt cgacgatgcg ccctgggccg aggttgtcga cccgcctctc acagtcatcg
     6061 cgcagccgac cgaggagatc ggagccctgg cggcacgtca gttgctcgat cgcctcggcg
     6121 gagagagcgg cgccccgatg aaccatcgct tgccggtgcg gttgatcgag cggcagtccg
     6181 tggcccgtcc tcctggctga cggcgggccg atggccgggc cagccgcccg gcaacctggt
     6241 gcagcgcgaa ctggctcttc gaacttaacc gggcatcgcg tggcagatga tggctgtttc
     6301 ccgtcggtgt tggcgggtgc aggcccagcg tatgcggcgt tcctggtttc gggtgttgc
//
LOCUS       DAOPJG010000186         6314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_13215_length_6314_cov_8.337274, whole genome shotgun sequence.
ACCESSION   DAOPJG010000186 DAOPJG010000000
VERSION     DAOPJG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13215_length_6314_cov_8.337274"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKU75_13975"
     CDS             <1..627
                     /locus_tag="PKU75_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPF82033.1"
                     /translation="YWGPELQKYGKQFREPKTIIYNGATQSACGTASNQVGPFYCPLD
                     ESIYIDASFFNILEQNFGSSSGKLAQEYVVAHEYGHHIQNVLGLLGQAQQDPQGATSG
                     AVRIELMADCFGGMWAQAASTTKDAQGNTFLKELTETDIRDALSAASAVGDDNIQAKT
                     QGRVTPENWTHGSAEARQRWFIQGMKSGNDINKCNTFNVQDPNSQVVS"
     gene            complement(708..1352)
                     /locus_tag="PKU75_13980"
     CDS             complement(708..1352)
                     /locus_tag="PKU75_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acVLRF1 family peptidyl-tRNA hydrolase"
                     /protein_id="HPF82034.1"
                     /translation="MVSTSSPSGAQATPRIIEVAPERLSAWLAGFAERHGGEPPRGVP
                     HSVTAPDGVVARFDYFDHDPFGVILVRRGGYAVGRVRNGRLLLHKVGTRYVQGKTAAG
                     GWSQQRYARRRENQADELVTAVADHAARILVDLAHVAEETGEIPVGGIPVTPGLVVGG
                     DRQLVGNVLSDKRLTSLRDLPRRSLYDIGDPNRAVLETAVKRATAVRISLGETP"
     gene            complement(1336..1665)
                     /locus_tag="PKU75_13985"
     CDS             complement(1336..1665)
                     /locus_tag="PKU75_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HPF82035.1"
                     /translation="MTENATLPANVADLEEAMRDVVDPELGINVVDLGLVYGITVDPQ
                     NHAIIDMTLTSAACPLTDVIEDQTAQALDGLVTTHRINWVWMPPWGPDKITDDGREQL
                     RALGFNI"
     gene            complement(1658..2107)
                     /locus_tag="PKU75_13990"
     CDS             complement(1658..2107)
                     /locus_tag="PKU75_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HPF82036.1"
                     /translation="MDLYQELIIDHSKRPIRMGLREPFDAEVHHVNPTCGDEITLRVD
                     RDGETLTDISYDSLGCSISVASASMLAELLTGEDLDTARHTYAAIRSMLTSKGEDPGD
                     EDVIGDAIALAGVAKYPARVKCALLSWMALADALAKTGVNLSEESHD"
     gene            complement(2109..3410)
                     /locus_tag="PKU75_13995"
     CDS             complement(2109..3410)
                     /locus_tag="PKU75_13995"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPF82037.1"
                     /translation="MTVHAPAPIAFGDQERARIRADFPILGRTVRHGHPLIYLDSGAT
                     SQKPYAVIDAERDWYERLNAAPHRGAHALSEEGTEAYEAARATVARFIGARPSEIVFT
                     KNATESINLAAYAFSNSAAGGGGRDDRFRLGPGDEIVVTEMEHHANLIPWQELSKRTG
                     ATLKWFSVDDDGRLDLSAIDDVITERTKVVAFVHASNVLGTINPVEVIVARAREVGAI
                     VVLDACQSVPHLPVDVTDLGVDLLAFSGHKMLGPMGVGVLWGKPEILEQMPVFLTGGS
                     MIETVSMDGATYAPVPQKFEAGVPNAAQAVGLAAACDYLSNLGMDKVSAHEHALTGIL
                     LDGLRERPWTRLIGPDSLENRGGAVSFVVDGVHAHDVGQILDDHGVAVRVGHHCAWPL
                     HRRFRAAATTRASLAPYNTPEEIGAFFTALDTVPGVFGIEV"
     gene            complement(3418..4176)
                     /gene="sufC"
                     /locus_tag="PKU75_14000"
     CDS             complement(3418..4176)
                     /gene="sufC"
                     /locus_tag="PKU75_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPF82038.1"
                     /translation="MSTLEIRDLHVSITADDVEKEILRGVTLTINSGETHAIMGPNGS
                     GKSTLAYSIAGHPKYTVTSGSVTLDGEDVLAMSVDERARAGLFLAMQYPVEVPGVTVS
                     NFLRTAKTAIDGEAPKLRTWVKDVKEAMGNLRMDPAFAERGVNEGFSGGEKKRHEVLQ
                     LELLKPRFAILDETDSGLDVDALRVVSEGVNRAQETNDMGVLLITHYTRILRYIKPQF
                     VHVFVDGVIAEQGGPELADRLEDEGYDRFATAGV"
     gene            complement(4182..4583)
                     /locus_tag="PKU75_14005"
     CDS             complement(4182..4583)
                     /locus_tag="PKU75_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HPF82039.1"
                     /translation="MTPDHATTDHAAADPVTTQAELVPVAVCRLDELPDVGAAKADIY
                     GQPVAIVHTEDGDVYAIDDICSHANVSLSEGELEGCQLECWLHGSRFDVRTGAPSGLP
                     ATEPVATFPVTIEDGEVFVSVPASLIPDSEK"
     gene            complement(4580..5761)
                     /gene="sufD"
                     /locus_tag="PKU75_14010"
     CDS             complement(4580..5761)
                     /gene="sufD"
                     /locus_tag="PKU75_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPF82040.1"
                     /translation="MSLLTEKTQRPGPAPHTDAAAAFVPDQSRAERTRSYAVADFPMP
                     TGREEEWRFTPVDRLRMLLRDEPTGLCLDWDESLPAGVRLGTMSVEDWRASGAPAPGD
                     RAAAIAAAHSGGVAVLDIPGDAVLTEPVRITLSGNDGQLVHGHLLMRAGRFSKATVVL
                     THAGHAAYSEVLTVLAGDGADLTVVTLQEWDDRSQHLAEHDVVVGRDATVKHIAVTLG
                     GGIVRLNTNATYAGPGGTFEGYGVYFADAGQHQEHRLFVDHEAPHCRSNVEYKGALQG
                     EDAHTVWVGDVLIRAAAEGTDTYELNRNLLLTDGARADSVPNLEIETGEIAGAGHASA
                     TGRFDDQQLFYLMARGIPEAEARRLVVRGFFATVVSKIGVPEVIGHLMTAIDQELEGA
                     Q"
     gene            complement(5765..>6314)
                     /locus_tag="PKU75_14015"
     CDS             complement(5765..>6314)
                     /locus_tag="PKU75_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPF82041.1"
                     /translation="MEWIDGNIGSKVTMKYPAVYLLGQHAKGETLSIAFAGEGQHQDA
                     GSKMVHAAPNTSSQIISKSVARGGGRTSYRGLVQVMEGATGSKSSVVCDALLVDTISR
                     SDTYPYVDIREDDVQMGHEASVSKVSEDQMFYLMSRGLSEEEAMAMIVRGFVEPIARE
                     LPMEYALELNRLIELQMEGAVG"
BASE COUNT          972 a         2025 c         2266 g         1051 t
ORIGIN      
        1 tattgggggc ccgagctgca gaaatacggc aagcagttcc gcgaacccaa gacgatcatc
       61 tacaacggcg cgacgcagtc ggcgtgcggc accgcctcca accaggtcgg cccgttctat
      121 tgccccctcg acgagtcgat ctacatcgac gccagcttct tcaacatcct cgagcagaac
      181 ttcggctcct ccagcggcaa gctggcccag gagtacgtcg tggcacacga gtacggccac
      241 cacatccaga acgtcctcgg actgctcggc caggcccagc aggatccgca gggtgcgacc
      301 tctggtgcgg tgcgcatcga gctgatggcc gactgcttcg gcgggatgtg ggcgcaggcc
      361 gcctccacga ccaaggacgc ccagggcaac accttcctga aggagttgac cgagaccgac
      421 atccgcgacg cgctctcggc tgcatcggcg gtcggcgacg acaacatcca ggccaagacc
      481 cagggccggg tcacccccga gaactggacc cacggctcgg ccgaggcccg gcagcgctgg
      541 ttcatccagg gcatgaagtc gggcaacgac atcaacaagt gcaacacctt caacgtccag
      601 gacccgaaca gccaggtcgt cagctgaggg tcactcgaca atggcgaagg cccccaccgg
      661 gaattccggt gggggccttc gtcgtggagg agcggggtga gtgccggtca gggggtctcg
      721 cccagactga tccgcacggc ggtcgcccgc ttgacggcgg tctcgagcac ggcccggttg
      781 ggatcgccga tgtcgtagag gctgcgccga ggcagatcgc gcaacgaggt aagccgcttg
      841 tcgctcagca cgttgccgac gagttgccgg tcaccgccga cgacgaggcc gggtgtcacg
      901 gggatcccgc ccaccgggat ctcgccggtt tcctccgcga catgggcgag gtcgacgagg
      961 atgcgcgccg cgtggtctgc cacggccgtg acaagctcgt cggcctggtt ctcgcgccgt
     1021 cgcgcatagc gttgctgcga ccagccgccg gcagcggtct tgccctgcac gtagcgcgtc
     1081 ccgaccttgt gcaggagcag cctgccgttg cggacgcggc ccacggcata cccgccccgg
     1141 cgcaccagga tcacgccgaa cgggtcgtga tcgaagtagt cgaagcgcgc aacgaccccg
     1201 tcgggtgcgg tgacgctgtg cggcacgccg cgcggaggtt cgccgccgtg gcgctcggcg
     1261 aagccggcga gccatgcgga caggcgctcc ggggcgacct cgatgatgcg aggggtcgcc
     1321 tgggcgccac tcgggctaga tgttgaaacc aagggcacgt agttgctccc ggccgtcgtc
     1381 ggtgatcttg tcgggtcccc atggtggcat ccagacccag ttgatgcgat gcgttgttac
     1441 caaaccgtca agtgcttgtg ccgtctggtc ctcgatgacg tcggtcagcg ggcacgccgc
     1501 ggacgtcagc gtcatgtcga tgatcgcgtg attctgcggg tcgacggtga tgccataaac
     1561 gaggccgagg tcgacgacat tgatcccgag ttcggggtcg acgacatcgc gcatggcttc
     1621 ttccaggtcg gccacattcg ctgggagagt ggcgttttca gtcatgtgac tcctccgaaa
     1681 ggttgactcc ggtcttggcc agcgcgtcgg cgagggccat ccacgacagc agggcgcact
     1741 tgacgcgcgc gggatatttg gcgacgccgg cgagcgcgat cgcgtcgccg atgacgtcct
     1801 cgtcgccggg atcttcgccc ttggacgtca acatcgaccg aatcgccgcg taggtgtgac
     1861 gcgccgtgtc gagatcctcg cccgtgagca gttcggcgag catcgacgcc gaggcgacgg
     1921 agatcgagca gccgaggctg tcgtaggaga tgtcggtgag cgtctcgcca tcgcgatcga
     1981 cgcgcagcgt gatctcgtcg ccgcacgtcg ggttgacgtg gtgcacttcg gcatcgaacg
     2041 gctcgcgcag gcccatgcgg atgggtcgct tcgaatgatc gatgatcagt tcttggtaga
     2101 ggtccacctc agacctcgat cccgaagacg cccggcaccg tgtcgagggc ggtgaagaag
     2161 gcgccgatct cctcgggggt gttgtagggc gccaggctgg cacgcgtcgt cgccgccgcg
     2221 cggaagcgac ggtgcaacgg ccaggcgcag tgatgtccca ctctcactgc aacaccgtgg
     2281 tcgtcgagaa tctgcccgac gtcatgggcg tggacgccgt cgacgacgaa actcactgct
     2341 cccccgcggt tttcgaggct gtccgggccg atcaggcggg tccaggggcg ctcgcgcagg
     2401 ccgtcgagca gtatgccggt cagggcgtgc tcgtgggcgc tcaccttgtc catgccaagg
     2461 ttggagaggt agtcgcaggc cgcggcgagc ccgacggctt gggcggcgtt ggggacgccg
     2521 gcctcgaact tctgcggcac gggcgcatag gtggcgccgt ccatgctgac ggtctcgatc
     2581 atcgacccgc cggtgaggaa gacgggcatc tgctcaagga tctcgggctt gccccacagg
     2641 acaccgacgc ccatggggcc gagcatcttg tggccggaga aggccagcag gtcgaccccg
     2701 aggtcggtga cgtcgacggg caggtgcggc accgactggc acgcgtccag gacgacgatc
     2761 gcgccgacct cccgggcacg cgcgacgatc acctcgacgg ggttgatggt gccgaggaca
     2821 ttggaggcgt ggacgaaggc cacgaccttg gtgcgctcgg tgatgacgtc gtcgatggcc
     2881 gacagatcga gccggccgtc gtcgtcgacc gagaaccact tcagcgtcgc cccggtgcgc
     2941 ttcgaaagct cctgccacgg aatgaggttg gcgtggtgtt ccatttcggt gacgacgatc
     3001 tcgtcgccgg ggccgagccg gaagcgatca tctcggccgc cgccgccggc tgccgagttg
     3061 gagaaggcgt aggccgccag gttgatcgac tcggtggcgt tcttggtgaa gacgatctca
     3121 ctcggccggg ctccgatgaa gcgggcgacc gtggcgcggg ccgcctcata cgcctcggtg
     3181 ccctcctcgg acagcgcgtg cgcgccgcgg tgcggggcag cattgagccg ctcgtaccaa
     3241 tcgcgttcgg cgtcgatgac ggcatacggc ttctgggagg tggcacccga gtcgagatag
     3301 atgagcgggt gcccgtggcg caccgtgcgg cccaggatcg ggaagtcggc gcggatgcgc
     3361 gcgcgttcct gatcgccgaa cgcgatcggt gccggtgcgt ggacggtcat ggccgggtca
     3421 gactccggcg gtggcgaagc ggtcgtagcc ctcgtcctcg aggcggtcgg cgagttcggg
     3481 gccgccctgc tcggcgatga cgccgtcgac gaagacgtgg acgaactgcg gcttgatata
     3541 gcgcaggatc cgcgtgtagt gggtgatgag cagcaccccc atgtcattgg tctcttgggc
     3601 gcggttgacg ccctcggaca ccacgcgcag ggcgtcgacg tcgagaccgg agtcggtctc
     3661 gtccaggatg gcgaagcggg gcttgagcag ttcgagttgc agcacctcgt ggcgcttctt
     3721 ctcgccaccg gagaagccct cgttgacacc acgctcggcg aaggcggggt ccatgcgcag
     3781 gttgcccatg gcctctttga cgtccttgac ccaggtgcgc agcttggggg cctcgccgtc
     3841 gatcgcggtc ttggcggtgc gcaggaagtt gctgaccgtc acgccgggga cctcgacggg
     3901 gtactgcatg gcgaggaaga ggccggcgcg ggcgcgctcg tcgacgctca tcgcgagcac
     3961 atcctcgccg tcgagggtga ccgagccgga cgtgaccgtg tatttggggt gccccgcaat
     4021 ggaataggcg agcgtcgact tgccagagcc gttggggccc atgatcgcgt gggtctcgcc
     4081 ggagttgatg gtcaacgtga ccccgcgcag gatctccttc tcgacgtcgt cggccgtgat
     4141 ggagacgtgc aggtcgcgga tctcaagggt ggacatggtt ttcacttctc gctgtcggga
     4201 atcagggacg cggggacgga gacgaagact tcgccgtcct cgatggtcac ggggaaggtg
     4261 gcaacgggct cggtcgccgg caggccgctg ggcgcgccgg tgcgcacgtc gaatcgggag
     4321 ccgtggagcc agcattcgag ctggcacccc tcgagttcgc cttcgctcag agagacattg
     4381 gcgtgactgc agatgtcgtc gatcgcatac acgtcgccgt cctcggtgtg gacgatggcc
     4441 accggctgcc catagatgtc ggccttggcg gcacccacgt cggggagctc gtcgaggcgg
     4501 cagacggcga cggggaccag ctcggcctgg gtggtcacgg ggtcggcggc tgcgtggtca
     4561 gtggtcgcgt ggtcgggagt cactgggcgc cctccaactc ctggtcgatg gccgtcatca
     4621 ggtggccgat gacctcgggc acgccgatct tcgagacgac ggtcgcgaag aagccgcgca
     4681 cgacgaggcg ccgggcttcc gcctcgggga tgccacgggc catcaggtag aagagctgct
     4741 ggtcgtcgaa acggcctgtc gcagaggcgt gtccggcgcc ggcgatctcg ccggtctcga
     4801 tctcgaggtt gggcacggag tccgcgcggg ctccgtcggt cagcagcagg ttgcggttga
     4861 gttcgtaggt gtcggtgccc tcggcggcgg cgcggatgag gacgtccccg acccagacgg
     4921 tgtgggcgtc ctcgccctgc agcgcgccct tgtactcgac attggagcga cagtgcggcg
     4981 cctcgtggtc gacgaacagc cggtgctcct ggtgctggcc ggcgtcggcg aagtagaccc
     5041 cgtagccctc gaaggtgccg cccggaccgg catacgtcgc gttggtgttc aggcgcacga
     5101 tgccgccgcc gagggtgacg gcgatgtgct tgaccgtggc gtcacggccg acgacgacgt
     5161 cgtgctcggc gaggtgctgg ctgcggtcgt cccactcctg cagggtgacg acggtcaggt
     5221 cggcgccgtc gccggcgagc accgtgagca cttcgctgta tgccgcgtgg cccgcgtgcg
     5281 tgagcaccac cgtggccttg ctgaagcggc cggcgcgcat caggaggtgg ccgtggacga
     5341 gctggccgtc gttgccggac aacgtgattc gcaccggctc ggtcagcacg gcatcgccgg
     5401 ggatgtcgag caccgccact cccccgctgt gcgccgccgc gatggcggcc gcgcggtcgc
     5461 cgggcgccgg cgcgccgctg gcgcgccagt cctcgacgct catcgtgccc aggcgcacac
     5521 ccgccgggag cgactcgtcc caatcgaggc agagcccggt cggctcgtcg cgcagcagca
     5581 tacgaagccg gtccacgggg gtgaaccgcc actcctcctc ccggccggtg ggcatgggga
     5641 agtcggccac ggcatacgac cgggtgcgct cggcgcgcga ctggtcgggc acgaacgcag
     5701 ccgctgcatc ggtatgcggc gccggcccgg gtcgctgggt cttctcagtc aggagcgaca
     5761 tggatcagcc gaccgcgcct tccatttgca gttcgatgag acggttgagt tcgagggcgt
     5821 actccatggg gagttcgcgg gcgatcggct cgacgaagcc gcgcacgatc atcgccatcg
     5881 cctcctcttc ggacaggccg cgggacatca ggtagaacat ctggtcctcc gagactttcg
     5941 agacggacgc ctcgtggccc atctggacgt cgtcctcgcg gatgtcgaca taggggtagg
     6001 tgtcggagcg gctgatcgtg tcgacgagca gggcgtcgca gacgacgctg gacttgctgc
     6061 ccgtcgcgcc ctccatcacc tgcacgagcc cgcggtatga cgtgcggccg ccgccgcgtg
     6121 ccaccgactt gctgatgatc tggctggagg tgttgggcgc ggcgtgcacc atcttggagc
     6181 cggcgtcctg gtgctggccc tcgccggcga aggcgatcga cagggtctcg cccttggcgt
     6241 gctggccgag caggtagacc gcggggtact tcatcgtcac cttggagccg atgttgccgt
     6301 cgatccactc catc
//
LOCUS       DAOPJG010000187         6298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_13264_length_6298_cov_7.487586, whole genome shotgun sequence.
ACCESSION   DAOPJG010000187 DAOPJG010000000
VERSION     DAOPJG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13264_length_6298_cov_7.487586"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..833)
                     /locus_tag="PKU75_14020"
     CDS             complement(69..833)
                     /locus_tag="PKU75_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF82042.1"
                     /translation="MTAVLEARGISRKFGHVQALDSADFDVFPGEVVALIGDNGAGKS
                     TLVKAITGNLEIDAGEVLFDGNPVTIATPQDASELGIEVVHQDLALAPHLGPAQNMYL
                     GREIMRPGLLGKLGFMDTGRMRRGAADAFRDLGSAVRSVVTPVGEMSGGQKQSVAIAR
                     SVAWADRVLFLDEPTAALGVVQTQNVLDMIRRVRDKGLGVVLISHSMPEVLDVADRVQ
                     VLRLGHRVATYQAKETTIEELVGAMTGALDRREDVA"
     gene            complement(830..1906)
                     /locus_tag="PKU75_14025"
     CDS             complement(830..1906)
                     /locus_tag="PKU75_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF82043.1"
                     /translation="MGNGAAGVTIYDVAQAAGVSIKTVSRVINGAPNVRPGTAAAVQR
                     AIDDLGYRPNAAARSLAAGRNDTIGVIVDRLQDPFFGQIVAVAEARALELGMDVLVAS
                     TGVEGHRATAHLARLVQRGVAGMLIAPFGDDDPIWREIPRDRPVVLIDRRCGVQGLDV
                     VRADDEQAAKAAVEHLITHGHKRIAFLGAPLRFSTVSDRLDGYLAALAAHGIPADPTI
                     IETQCADATDSTQLLRPRLDAAAPPTAVFSSTPMIAEGVVRALALAGRRDVAHVAFGD
                     FPLADVTSPPTTVIDQCPETLAHAAMDRLLARISGTELAPEDIVLPTRLIPRGSGELL
                     PPPDAPQTRNDMKRTKVTAMEVRQ"
     gene            2091..2819
                     /locus_tag="PKU75_14030"
     CDS             2091..2819
                     /locus_tag="PKU75_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF82044.1"
                     /translation="MSRFPDGLYLVTGASGGIGAATVRRLVAEGARVAASGRDQAKLQ
                     ELADETGCEALPFDLMDEDSIRAAVSGRDWFGIVNCGGDGGEIATPMDTDIDVFDRVM
                     AVNARGALLVIKYATPSMIEAGRGGSIVNVSSQASLVALEGHISYGSSKAALDNITRV
                     SALELGKYGIRVNSVNPTVIMTPMSEWYWGRPDVGGPFLEQMPMHKWATVEDVSGPIA
                     FLLSDDAAMISGVSLPIDGGFTSR"
     gene            2816..4189
                     /locus_tag="PKU75_14035"
     CDS             2816..4189
                     /locus_tag="PKU75_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPF82045.1"
                     /translation="MSAPLVAGLDIGSTNVKVQITDLDGREIVGADRPTPWRVDANGT
                     ALEQESLRRTIEEVLHEAAGRLEKQEYAGASVGALAVAGMGESGFVLDDAGAALAPAI
                     AWFDARGAEQLDAFPSTLRAEFAARTGLPLGVQVPVIKLLWLRDNGIQLQGQQWQNVP
                     EYAAAALGGRRALERSLTARLGLIDQDAEGPWADLLAHIGIDASFLPPVVDSATDLGG
                     ANADWLPAAFAGARLAVAGHDHLVAATAGGATADGRYYGSFGTAEVLVRVLDTPLSAD
                     ARARLAEFFINHVPHVEAGRYVLVAGVKTGLIMRRILHLAGITDREARDRLDREVMAL
                     PVEGSLPRGAVTVGGARNDDGILALRVAHDGAGPAEVFAAALRHGNDELARLIEAMDR
                     ELPPATGSVLAGGWVGMNSVGRARSLVLPDLHLSTRAQDTAYGAALTAARLLTTNSPT
                     PIEGENP"
     gene            4186..5076
                     /locus_tag="PKU75_14040"
     CDS             4186..5076
                     /locus_tag="PKU75_14040"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82046.1"
                     /translation="MSPMTTIERRALQAISTPNGHMLIVAADQRNSMKAVMTDAPDGP
                     GSVTPDQLIEAKADLVRHLGNNAPAILLDPETALPQVVDDGTLARGSALVVGMDQSGY
                     ETVDGLRFTRVVEGVTPRRVRDLGGDAAKMLWYIRPDKQDANSLVGQEIRELTAACEA
                     EGVLLIVEILTYRLEGETEEDYRAAFPGFIADVARLSVECGAKVLKLPYPGSAEACAA
                     VTEAAAGAPWAVLSAGVDHETFIGQVRTAVANGCAGAMAGRSLWKDSLAVDPQVRTQL
                     LTERALPRLRELAVTVDGAA"
     gene            complement(5189..>6298)
                     /locus_tag="PKU75_14045"
     CDS             complement(5189..>6298)
                     /locus_tag="PKU75_14045"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPF82047.1"
                     /translation="FDITAASEVMVLLTLATSLDDLKARLRRIVVGYAYDGRPVTADD
                     LGAAGAMAVILKDALNPNLLQTIEGTPALIHCGPFGNIATGSSSVVADIVATRRADIV
                     LTEAGFGSDMGAERFFNVKCRVAGLRPDAAVLVVTVRALKAHSGRFKITPGKALPPEL
                     LAENPGDVRAGIANLRKHIQIIEGFGIWPVVAINAFDTDFDSEHQVIRDLCTEMGVKC
                     AVSTHVVNGGAGAEDLARTLVDALRTPSRFRFTYASDAHLTAKIEAVAKQVYGADGVD
                     FAPPAAKALAEYERLGFGGLPVVIAKTHLSISHDPSLLGAPTGWRLPVREVRAAAGAG
                     YVYAICGDMRTMPGLSSHPNAEKIDLVDGEIVGLS"
BASE COUNT          948 a         2096 c         2253 g         1001 t
ORIGIN      
        1 cgaggtcgat tgctcggcta cggggtcggg gctgcgcggc gccggtgcgt tggtcacgtc
       61 gtcgttgctc atgccacgtc ctcccttcgg tccagggcac cggtcatggc accgaccagt
      121 tcttcgatgg tggtctcctt ggcctgatag gtcgcgacgc ggtggccgag ccgcagcacc
      181 tgcacccggt cggcgacgtc gaggacctcg ggcatcgagt gcgagatgag caccacgccc
      241 agccccttgt cgcgcacccg ccggatcatg tcgagcacgt tttgcgtctg gaccacgccc
      301 agcgcggcgg tgggctcgtc gaggaagagg acgcggtcgg cccacgccac ggagcgggcg
      361 atggcgacgc tctgcttctg gccgccggac atctcaccga ccggggtgac gaccgaccgg
      421 accgcggacc cgaggtcgcg gaaggcgtcg gcggcaccac gccgcatacg gcccgtgtcc
      481 atgaaaccga gcttgcccag gagtccgggg cgcatgatct cgcgaccgag atacatgttc
      541 tgagcggggc cgagatgggg cgcgagcgcg aggtcttggt ggacgacctc gatgccgagc
      601 tcggacgcgt cctgtggggt cgcgatggtc accggattgc cgtcgaagag cacttcgccg
      661 gcgtcgatct ccaggttgcc ggtgatcgcc ttcacgagcg tcgacttgcc ggccccgttg
      721 tcgccgatga gggcgacgac ttcgccgggg aagacgtcga agtccgcact gtcgagcgcc
      781 tggacgtgcc cgaacttgcg gctgatgccg cgtgcctcca ggacggcggt cattgtcgta
      841 cctccattgc tgtcaccttc gtgcgcttca tgtcgttcct tgtctgcggt gcgtcggggg
      901 gcggcaggag ttcgccggag ccgcgcggga tcagccgcgt gggcaagacg atgtcctcgg
      961 gggcgagctc ggtgccggag atgcgggcga gcaatcggtc cattgccgcg tgggccaggg
     1021 tttctgggca ctggtcgatg acggtggtcg ggggggaggt gacgtccgcg agcgggaagt
     1081 caccgaaggc cacatgggcc acgtcccgac gcccggccag tgccaaagcg cgaaccacgc
     1141 cttcggcgat catgggcgtg ctggagaaga cggcggtggg gggcgctgcc gcatccaggc
     1201 gggggcgcag tagttgcgtc gagtcggtcg cgtccgcgca ctgcgtctcg atgatggtgg
     1261 gatcggcggg tatgccgtgc gccgccagcg cggcgagata accgtccagg cgatcactga
     1321 ccgtgctgaa gcgcaggggc gcgcccagga aggcaatgcg cttgtgcccg tgcgtgatca
     1381 ggtgctcgac ggcggctttc gccgcttgct cgtcgtcggc ccggacgacg tcgaggccct
     1441 ggacgccgca gcgtcggtcg atgaggacca ctgggcggtc ccgcgggatc tcccgccaga
     1501 tcgggtcgtc gtcgccgaag ggggcgatga gcatgccggc cacaccacgc tgcacgagcc
     1561 gagcgaggtg agcggtcgcc cgatggccct cgactcccgt cgaggcgacg aggacgtcca
     1621 ttccgagctc gagcgcgcgc gcctcggcga cggcgacgat ctggccgaag aaagggtcct
     1681 gcagacggtc gacgatgacg ccgatggtgt cgttgcggcc cgccgcgaga ctgcgtgccg
     1741 cggcgttcgg gcggtatccg aggtcgtcga tcgcgcgctg gacagcggct gcggtcccag
     1801 ggcggacatt gggggcgccg ttgatgacgc gggacacggt cttgatggag acgcctgcgg
     1861 cctgggcgac gtcgtaaatg gtcacccccg cagcaccatt ccccatgtcc aggcctcctt
     1921 gccggtgccg ggaacgctgc tcggtgggtc ggatcccacc gttgccattt cccggattag
     1981 cgctaagcta cgactggatg acagcgctgt caagagtctt gatcggcgtt actcggttgt
     2041 tatgacgagc gcatccgctg ttcgtgccac ccgccaacaa ggagcgtcca atgtctcgat
     2101 tccccgatgg tctctatctc gtcaccggtg ccagcggtgg catcggcgct gcgacggtgc
     2161 gtcgcttggt tgcggagggt gctcgcgtcg ccgcgagtgg ccgcgaccag gccaagctcc
     2221 aggagctcgc cgacgaaacc ggctgtgagg ctttgccttt cgacctgatg gacgaggact
     2281 cgatccgtgc ggccgtgtcc ggtcgcgact ggttcggcat cgtcaactgc ggtggggacg
     2341 gtggcgagat cgcgacgccg atggacaccg acatcgacgt tttcgatcgc gtcatggccg
     2401 tcaacgcccg tggggcgctg ctcgtcatca agtacgccac gccctcgatg atcgaggccg
     2461 gccggggtgg ctcgatcgtc aacgtctcga gccaggcctc cctcgtcgcc ctcgaaggcc
     2521 acatctccta tggctcgtcc aaggccgccc tcgacaacat cacccgggtc tccgcgctcg
     2581 agctgggcaa atacggcatc cgggtcaata gcgtcaatcc gacggtgatc atgacgccga
     2641 tgtcggagtg gtactggggc cggcccgatg tcggcgggcc cttcctggag cagatgccca
     2701 tgcacaagtg ggccaccgtc gaggacgtct cgggccccat cgccttcctc ctcagcgacg
     2761 acgccgcgat gatcagcggc gtcagcctgc cgatcgacgg cggcttcacc tcccggtgag
     2821 cgcgccgctc gtcgccggtc tcgacatcgg cagcaccaac gtcaaggtcc agatcacgga
     2881 tctcgacgga cgcgagatcg tgggcgccga ccggccgacg ccctggcggg tcgacgccaa
     2941 cgggaccgcg ttggagcagg agagcctgcg gcgcaccatc gaggaggtgc tgcacgaggc
     3001 cgccgggcgg ctcgaaaagc aggagtatgc cggtgcctcg gtcggtgcgc tggctgtcgc
     3061 cggcatgggc gagagcggct tcgtcctcga tgatgccggc gccgcgctgg cacccgcgat
     3121 cgcgtggttc gacgcgcgcg gcgccgagca gttggacgcc tttccgtcga ccttgcgcgc
     3181 cgagttcgcc gcccgcaccg ggctgccgct cggagtccag gttcccgtca tcaagctatt
     3241 gtggttgagg gacaacggaa ttcagctgca agggcagcag tggcagaacg tcccggagta
     3301 tgccgccgcc gcgcttgggg gtcgccgcgc cctcgagcgt tccctgacgg cacgcctggg
     3361 cctgatcgat caggacgccg aaggtccgtg ggcagatctg cttgcgcaca tcggcatcga
     3421 cgcctcgttc ctgccgccgg tggtggactc ggcgaccgat ctcggtgggg cgaatgctga
     3481 ctggctgccg gcagcatttg cgggcgctcg actcgccgtt gccgggcacg accacttggt
     3541 cgcggcgacc gccggcggcg ccaccgcgga cggccgctac tacggatcct tcggcaccgc
     3601 cgaggtgctc gtgcgtgtcc tggacacacc cttgagcgcg gacgcccgag ctcggctggc
     3661 cgagttcttc atcaaccatg tcccccacgt cgaggccggg cgctatgtgc tcgtcgccgg
     3721 cgtcaagacc gggctgatca tgcgccgcat actgcacctg gccggcatca ccgaccggga
     3781 ggcgagggac cggctcgacc gcgaggtcat ggcattgccc gtcgaagggt ccctgccgcg
     3841 cggagccgtc acggtcggcg gtgcgcgcaa tgacgacggc atcctcgcgc tgcgggtcgc
     3901 gcacgacggc gcgggaccgg cggaggtctt cgcggccgcg ctccgccacg gcaacgacga
     3961 actcgcccgg ctcatcgagg cgatggaccg cgaactgccc cccgcgaccg gctcggtgct
     4021 ggccggcggg tgggtcggca tgaacagcgt cggccgggcc cggtccctcg tgctgccgga
     4081 cctgcacctt tccacgcggg cgcaggacac cgcatacggc gcggctctga cggccgcgcg
     4141 tctcctcacc acgaactcac caacgcccat cgaaggggaa aacccatgag tcccatgacc
     4201 accatcgaac gccgtgcgct gcaagcgatt tcgactccaa acgggcacat gctcatcgtg
     4261 gcggccgacc agcgcaacag catgaaggcg gtgatgaccg acgcacctga cggccccggc
     4321 tcggtcacgc cggaccagct catcgaggcc aaggccgacc tcgtgcgtca cctcggcaac
     4381 aacgccccgg ccatcttgct cgaccctgag accgccctgc cgcaggtcgt cgacgacggc
     4441 acgctggccc gcgggagtgc gctcgtggtc ggcatggatc agtcgggtta cgagacggtc
     4501 gacggcttgc gtttcacgcg ggtggtcgag ggggtcacgc cccgccgagt gcgcgacctc
     4561 ggcggtgacg ccgccaagat gctctggtac atccggccgg acaagcagga cgcgaattcg
     4621 ctcgtggggc aggagattcg cgaactgacg gccgcgtgcg aggccgaggg cgtgctgttg
     4681 atcgtcgaga tcctgaccta ccgtttggag ggagagacgg aggaggacta ccgcgcggcg
     4741 ttccccggct tcatcgccga cgtcgctcgg ctgagtgtcg agtgcggagc gaaggtgctc
     4801 aagctgccat accccggctc ggccgaggcc tgcgcggcgg tgaccgaggc ggcggccggc
     4861 gccccgtggg ccgtgctgtc cgccggcgtc gatcacgaga ccttcatcgg acaggtccgc
     4921 acggccgtgg ccaatggctg cgcgggcgcg atggccggcc ggtcgctgtg gaaggacagc
     4981 ctcgccgtcg atccgcaggt ccgcacgcaa ctgctcaccg agcgcgccct cccgcgcctg
     5041 cgcgagttgg ctgtcaccgt tgacggcgcc gcctgatccc cgtcgagtga acaccgccac
     5101 gcgatcgagt gaacatgctg tcgggtaacg acccgacagc atgttcactc gatgtgctcg
     5161 cggtgttcac tcgacgcgag gtgggggcct agctcaggcc gacgatctcg ccgtcgacga
     5221 ggtcgatctt ctcggcgttc ggatggctgg acaggccggg catggtgcgc atgtcgccgc
     5281 agatggcata gacgtagccc gcgcccgccg cggcgcgcac ctcgcggacc ggcaggcgcc
     5341 atcccgtcgg ggcgccgagc agcgacgggt cgtgcgagat cgacaggtgg gtcttggcga
     5401 tgacgaccgg caggccaccg aagccgagcc gctcgtactc ggcgagggcc ttggccgccg
     5461 gcggggcgaa gtcgacgccg tcggcgccgt agacctgctt ggccaccgcc tcgatcttcg
     5521 cggtcaggtg ggcgtcggag gcataggtga accggaaccg gctcggtgtg cgcagcgcgt
     5581 cgacgagcgt gcgcgcgaga tcctcggcgc ccgcaccccc attgacgaca tgggtcgaga
     5641 cggcgcactt gacgcccatc tcggtgcaca ggtcgcgaat gacctgatgc tcggagtcga
     5701 agtcggtgtc gaaggcgttg atggcgacga ccggccagat gccgaagccc tcgatgatct
     5761 gaatgtgctt gcgaagattg gcgattccgg cgcgtacgtc gccggggttc tcggcgagca
     5821 gctccggcgg caacgctttg cccggggtga tcttgaagcg tccggagtgc gccttgaggg
     5881 ctcgcacggt cacgacgagc acggcggcat cgggccgcaa ccctgccacc cggcacttga
     5941 cgttgaagaa ccgctcggcg cccatgtcgg agccgaagcc ggcctccgtg agcacgatgt
     6001 cggcccggcg ggtggcgacg atatcggcga cgacggagga ggagccggtg gcgatattgc
     6061 cgaacgggcc gcagtggatg agggccggcg tgccctcgat cgtctggagc agattggggt
     6121 tgagcgcgtc cttgaggatc acggccatcg ccccggcggc gccgaggtcg tcagcggtga
     6181 ccggccggcc gtcatacgca taacccacga cgatgcgccg caggcgcgcc ttcaaatcgt
     6241 ccagcgaggt cgcgagcgtc agcagcacca tgacctccga cgccgctgtg atgtcgaa
//
LOCUS       DAOPJG010000188         6221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_13475_length_6221_cov_8.036977, whole genome shotgun sequence.
ACCESSION   DAOPJG010000188 DAOPJG010000000
VERSION     DAOPJG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13475_length_6221_cov_8.036977"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..283
                     /locus_tag="PKU75_14050"
     CDS             20..283
                     /locus_tag="PKU75_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82048.1"
                     /translation="MRMPASLWGTGSGVTRALLDGAVDYAKSKGAKVIDGFPVDNQGD
                     TIDRTLAYPGMRTIFDDAGFTPLGEVRGNHAGHKRIAMRRTFD"
     gene            complement(382..1944)
                     /locus_tag="PKU75_14055"
     CDS             complement(382..1944)
                     /locus_tag="PKU75_14055"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HPF82049.1"
                     /translation="MAVHPSTPRDPGSGDLGISDVLNLVRDCTAALSSVRQEVGAATE
                     AQLLDLVSELGELALWSDAAEVAVVGDALTRGLPAASQVPLSPADWIATASRRHTRGS
                     ASRVVKIAEALRSPTITRPAGALAPGQDGEVLGEAILGAVVPLPCGQVAMDEMRRLAP
                     DLQPAFVPAAWEAYALVGAVGDPREVRRIRPELYARFGRPDRLNDRDEGARKHAFLST
                     GAVDPVDGLTDYRMSLSAESAAILEAALDRLTKPQPGPDGGADPRSYPTRRADALIEL
                     VRRATTHGVVPTVAPDTGAGLQGDGPGHPAADRPAAERPAAGRPASQVSLIVRLSDLR
                     DEDGCATTITGLDAGRYLSIATARLLSCNTMLAPIIVDEHGTPIIIGHSKRLFTSQQV
                     RAMQLRDDGCSFLHCTRPAGWADAHHLIHWADGGATEIDNGALLCNYHHHVVHSRRLA
                     GWVTTDPPDDTLGRPPGSSETSDAEDLARRRDRGEPAAADRPAGPPRVHWDLTPGSYD
                     RLLAARAGGSAA"
     gene            complement(2169..3179)
                     /locus_tag="PKU75_14060"
     CDS             complement(2169..3179)
                     /locus_tag="PKU75_14060"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF009098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPF82050.1"
                     /translation="MRRPNVTVLGGGSWGSAIASIAARNAPTLLWARDEATVADINER
                     HVNSRYLDDLPLNPALRATNDLEHAIATADVLVLGVPSQACRGVLTEAAEHLRPWVPI
                     VSLVKGLEQGSRLRITEVVRETLPGHPVGVLNGPNIAREIAAGYAAAATLAMPDQQQA
                     ESLQNLLRSTMFRVYTSTDVVGVEVAGALKNIFAIATGFGDGLEAGDNTRAMVITRSL
                     RELSRLGVAMGGHEQTFSGLAGMGDLIATCTSPHSRNRRVGIRLAQGKTLPEIEAELK
                     QVAEGVKSAPVVMELSREHGVEMPIAREVDAVLNHGQTAVRAYRGLLREAPEHEFHGD
                     AW"
     gene            complement(3240..4202)
                     /locus_tag="PKU75_14065"
     CDS             complement(3240..4202)
                     /locus_tag="PKU75_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82051.1"
                     /translation="MTSLQFSSDALDRSRAWLAAHGRPLERVRLAQVLGTATPGQVAE
                     AVSAYANSDGGVGNALESDCRAPESSALGALTALDILRAHRVPGDHPLVGQVCRWLVE
                     SVESDRQGRLVWPFLPPSAQASTHAPWWDQSEPGQLAETFAGYAANPGVALTAHLWRR
                     EVAAPGSVPAEMLAQLTQQVRDVAADGVSAEEVNAHDALAHFAGEAATPADAREAVTA
                     YLRRVLPERVMATEADYATYGIHPLWIAPTPAHPLAAALADTLPMALDRTIASQQPDG
                     SWATFWDWGGWHPDVWPQAEQEWRGSLIVRNIAALLAHGRVVRP"
     gene            4405..4548
                     /locus_tag="PKU75_14070"
     CDS             4405..4548
                     /locus_tag="PKU75_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82052.1"
                     /translation="MSDVMTRAESDVLDQPPPEDFATDLFESPELGTSGFFPHVDPEE
                     SDT"
     gene            4624..>6221
                     /locus_tag="PKU75_14075"
     CDS             4624..>6221
                     /locus_tag="PKU75_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPF82053.1"
                     /translation="MTANTAKYPGQPTVINGNAAVAHVMSQVCGGVIGYPITPSTEIS
                     ELYEAARAEGGVNVWGKHAFFFEPEGEHSAQSGALGAALTGGAFISNASSSQGILYAM
                     ESHFVTVGKKIGGFVLQVAARVVSKHSLNVMAGHDDIYALLSSGYTVLFGANPQEAAD
                     LAAISYRASSLSLIPVANAMDGFSTSHMLSEALMPEPELLREYLGDPAGRIACPTLAQ
                     EMLFGAKGRVFQLEGFLRRHGRDIPADDVAALQAFLAAKADEVERDNDGELVGMTLAW
                     VPEELQGPWRRQWVNAWEKGTRQLVPSLVDVNNPGLTGPVQNQPDFQAGAVDHRTHFA
                     SAVPSLVRQAMAEYSELTGRTYTPVHTYDTDDAEVVMVGLGSVCDDVRAVLPYLREQG
                     IKAGLVSVKMLQPFPEAEVVAALAGKKSVIVLERSDQTALTSLVTAALFKAQQNAESQ
                     RHNGIPALAQSPVLTTAIFGLGGHDLQPRHLIAAFKALDAGTLPPLIYLGSQFFDKNP
                     DPHMAQLQERLRAANAVDFDDLIS"
BASE COUNT          987 a         2108 c         2181 g          945 t
ORIGIN      
        1 ggcgaagagt tcgccgtagg tgcgcatgcc ggccagcctg tgggggactg ggtcgggcgt
       61 cacccgagcg ctgctcgacg gggccgtcga ctacgcaaag agcaagggcg ccaaagtcat
      121 cgacggcttc cccgtcgaca accaggggga cacaatcgac aggacgctgg cctaccccgg
      181 gatgcgcacc atcttcgatg atgccggctt cacgcctctg ggtgaggtgc gcggcaatca
      241 cgcgggccac aaacgcatcg ccatgcgccg gacattcgac tgacagccag atgaatgaca
      301 gccagccgac tgacagggca gcggactgac agccggccga ctgacagggc agcggaaatc
      361 gccgcgacgg gacggcgagt gtcaggcggc gctgccgccg gcgcgggcgg ctagaagccg
      421 gtcgtaactg ccgggggtca agtcccagtg cacgcggggt ggcccggccg gccggtcggc
      481 agctgcgggc tcgccgcggt cccggcggcg cgcgaggtcc tcggcgtcgc tggtctcgct
      541 gctcccggga ggtcgtccca gcgtgtcgtc gggtgggtcg gtggtgaccc agccggcgag
      601 gcgtcgggag tggacgacgt ggtggtggta gttgcacagc aacgcgccgt tgtcgatctc
      661 ggtggcaccg ccgtcggccc aatggatcag gtgatgagca tcggcccagc cggccggcct
      721 ggtgcagtgc aggaaagagc acccgtcatc gcgcagctgc atggctcgca cctgttggct
      781 ggtgaaaagc cgtttgctgt gaccgatgat gatcggcgtg ccgtgctcgt ccacgatgat
      841 gggggcgagc atcgtgttgc acgacagcag ccgggcggtc gcgatcgaca ggtagcgccc
      901 ggcgtccagg ccggtgatgg tggtggcgca accgtcctcg tcgcgcaggt cactcaggcg
      961 cacgataagg ctcacctggc tggccggacg cccggcggct gggcgttcgg cggctgggcg
     1021 atcggcggct gggtggcccg ggccatcccc ctgtagcccg gcgccggtgt cgggcgcgac
     1081 ggtggggacg acgccgtgtg tcgtcgcgcg ccggaccagt tcgatcaagg catcggctcg
     1141 tcgggtgggg taggagcggg ggtcggcgcc cccgtcgggg ccgggttggg gtttggtgag
     1201 acggtcgagc gcggcctcga ggatcgcagc ggattcggcg ctgagagaca tccggtagtc
     1261 agtcaggccg tcgaccgggt cgacggcgcc ggtggagagg aacgcatgct tgcgggctcc
     1321 ctcgtcgcga tcgttcaacc ggtcggggcg gccgaagcgg gcatacagct cgggccggat
     1381 gcgccggacc tctcgtgggt cgccgactgc ccccacgagg gcgtaggcct cccacgctgc
     1441 cggcacgaag gcgggctgca gatctggtgc caggcgccgc atctcgtcca tggcgacctg
     1501 gccgcagggc agcggcacga cggcgccgag gatcgcctcg ccgagaacct cgccgtcctg
     1561 gccgggggcc aacgccccgg cgggtcgggt gatcgtcggc gaccgcaggg cctcggcgat
     1621 cttcaccacg cgggacgccg aaccgcgggt atgccgccgc gaggccgtgg cgatccagtc
     1681 ggcggggctc agaggcacct gagaggcggc gggcaggccg cgggtgagag cgtcgccgac
     1741 gacggcgacc tccgcggcgt cactccacaa ggcgagttcg cccagttcgc tgacgaggtc
     1801 gagaagttgc gcttcggtgg ctgcgccgac ctcctgacgc acggatgaca gcgcggcggt
     1861 gcagtcgcgc acgaggttca gcacgtcgct gatcccgagg tcaccggacc cgggatcgcg
     1921 tggtgtgctg gggtggactg ccatacctca tgctacatct gttcgactga tcgcgcaacc
     1981 cctatttcga gaaatattat tgtctcccaa gggattacgt gtgcgccgag cttatagcac
     2041 tgaccactga cagtggtgca gaatcgactc tgtccaggtc gcgtcggccg atgatcggcg
     2101 gtggttcacc gtgacacggc gcagtcgccg tgagaggtcc ggagcaccgg tcctgacaag
     2161 gggagcccct accaggcatc gccgtggaac tcgtgctccg gcgcctcccg cagcaatccc
     2221 cggtaggcac ggacggcggt ctggccgtgg ttgaggacgg cgtcgacctc gcgggcgatg
     2281 ggcatctcga cgccatgctc tcgggacagc tccatgacga cgggggcgga cttgacgccc
     2341 tcggcgacct gcttcagttc ggcctcgatc tcgggcagcg tcttgccctg agccaagcgg
     2401 atgccgacgc ggcggttgcg gctatggggg gaggtgcaag tggcgatgag atcgcccatc
     2461 ccggcgagtc cggagaaggt ctgctcgtgc ccgcccatcg cgactccgag ccgcgagagt
     2521 tcgcgcagcg accgggtgat gaccatggcc cgggtgttgt caccggcctc caggccgtcg
     2581 ccgaatccgg tcgcgatggc gaagatgttc ttcagcgccc cggccacctc gacacccacg
     2641 acatcggttg aggtgtagac gcgaaacatg gtggagcgca acagattctg cagtgactcc
     2701 gcctgctgtt ggtcaggcat cgccagcgtc gcggcggcgg cgtacccggc cgcgatttcg
     2761 cgcgcgatat taggcccgtt gagcacaccg acgggatggc ccggcagggt ctcgcggacg
     2821 acctccgtga tccgcagccg gctgccctgc tccaagccct tgacgagcga gacgatcggc
     2881 acccacgggc ggaggtgctc ggctgcttca gtgagaactc cccggcaggc ttgtgacggc
     2941 acgccgagca ccagcacgtc tgccgtcgcg atggcgtgct ccagatcgtt cgtcgcccgg
     3001 agcgcaggat tcaaaggcag gtcgtcgagg tagcgcgagt tgacgtgccg ctcgttgatg
     3061 tccgcgaccg tggcctcgtc gcgcgcccac aacagagtcg gcgcatttcg agcggcgatc
     3121 gaggcgatcg cactgcccca cgaccctccg ccgaggacag tgacattggg tcggcgcatg
     3181 agggctcctt cgggaatcgg cgaccggtgt ggcgccgagc gtaatgcccg aacccaccgc
     3241 tacgggcgaa ccacccggcc atgagccagc agggcggcga tattgcggac gatcagcgaa
     3301 ccgcgccatt cctgctcggc ctgtggccag acatccgggt gccagccacc ccagtcccag
     3361 aaggtcgccc acgagccgtc cggctgctgg ctcgcgatcg tccggtcgag agccatcggc
     3421 agcgtgtcgg ccagggcggc ggcgagcggg tgcgccggcg tgggcgcgat ccacagcggg
     3481 tggatcccgt atgtcgcgta gtcggcctcc gtcgccatca cccgctccgg aagcacccga
     3541 cggaggtatg ccgtgaccgc ctcccgcgcg tccgcgggcg tggcggcttc tccggcgaag
     3601 tgcgccaacg catcgtgggc gttcacttcc tccgccgaga caccgtcagc agccacatcg
     3661 cgcacctgct gggtgagttg ggccagcatc tcggcaggca cgctccccgg cgcagccacc
     3721 tcgcgccgcc acaggtgagc ggtcaacgcg acacccgggt tcgcggcata cccggcaaac
     3781 gtctcggcga gctgacccgg ctcgctctgg tcccaccagg gcgcatgcgt actggcctgg
     3841 gccgacgggg gaaggaacgg ccataccaag cgcccttgcc tatcggactc gacactctcg
     3901 acgagccatc ggcacacctg acccaccagc ggatggtcac ccggcacgcg gtgtgcccgg
     3961 aggatgtcga gcgccgtcag cgcaccgagg gcactggatt ccggtgcgcg gcagtcggac
     4021 tcgagtgcgt tgccgacccc gccgtcggag ttggcatagg cggacacggc ctcggcgact
     4081 tgaccggggg tggccgtgcc gagcacctgc gcgagccgca cccgctcgag aggccggcca
     4141 tgagcagcca gccaggcgcg cgagcggtcg agggcatcgg acgagaactg gagcgacgtc
     4201 atacccgcga ctctaggaac gaggaccgac acccgtcgcg gtgtggcgta atccactccg
     4261 ttccatcttg cgttcctccg cgcgaagtga cttgatgaaa aggtccacag cgaggacaag
     4321 cgtcggccac cacgtggtgc gccgcgcaag gggtcgaggt ttgccgcccg ctgccgatga
     4381 gtcacctacg actggaggca ggcgatgagc gacgtgatga cccgagcgga atcggacgtc
     4441 ctcgaccagc ccccgccgga ggacttcgcc acggacctct ttgaatcacc cgaacttggc
     4501 acgtcgggat tcttcccgca tgtcgacccc gaggagtccg acacctagga ctccaaccgc
     4561 gacagggccg cggtggcgcg cggccgacga gaccggatgt ggcaatccgg tgaaggaaac
     4621 gaaatgaccg cgaacacagc gaagtacccg gggcaaccca ccgtcatcaa cggcaacgcc
     4681 gccgtggctc acgtcatgag ccaggtctgc ggcggggtca tcggctaccc catcaccccc
     4741 tcgaccgaga tctccgagct gtatgaagcc gcgcgcgccg agggtggtgt caacgtctgg
     4801 ggcaagcacg cgttcttctt cgagcccgag ggcgagcact ccgcccagtc cggtgcgctc
     4861 ggcgcggccc tgaccggcgg cgcgttcatc tcgaacgcct cctccagcca gggcatcctc
     4921 tacgcgatgg agtcccactt cgtcaccgtc ggcaagaaga tcggcggctt cgtgctgcag
     4981 gtcgccgcgc gtgtggtgtc gaagcactcc ctcaacgtca tggccggcca cgacgacatc
     5041 tacgcgctgc tgtcctccgg ttacaccgtg ctcttcggcg ccaacccgca ggaggccgcc
     5101 gacctcgccg cgatctccta ccgcgcctcc tcgctctcgc tgatccccgt cgccaacgcg
     5161 atggacggct tctcgacgag ccacatgctc tccgaggcgt tgatgccgga gccggagttg
     5221 ctgcgcgaat atctgggtga ccccgccggc cggatcgcct gcccgacgct ggcgcaggag
     5281 atgctgttcg gcgccaaggg ccgggtcttc cagttggagg ggttcctgcg tcgccacggc
     5341 cgggacatcc cggcggatga tgttgcggcg ctgcaggctt tcctcgccgc caaggcggac
     5401 gaggtggaga gggacaacga cggagagctg gtgggtatga cgctcgcctg ggtccccgag
     5461 gaactccagg ggccgtggcg tcgccaatgg gtcaatgcgt gggagaaggg gacgcgccag
     5521 ctggtgccct ccctcgtcga cgtcaacaac cccggcctga ccgggccggt gcagaaccag
     5581 cccgacttcc aggccggcgc cgtggaccac cgcacccact tcgcctccgc cgtgccgtcg
     5641 ctggtgcgcc aggccatggc ggagtattcg gaactgacgg gccgcaccta caccccggtt
     5701 cacacctatg acaccgacga cgccgaggtc gtcatggtcg ggctcggctc ggtctgcgac
     5761 gacgtgcgcg ccgtcctgcc ctacctgcgc gagcagggca tcaaggccgg cctggtctcc
     5821 gtcaagatgc tgcagccctt ccccgaggcc gaggtcgttg ctgcgctcgc gggcaagaag
     5881 tccgtcatcg tcctggagcg ctccgaccag acggcgctga cgtccctcgt cacggcggcc
     5941 ctgttcaagg cgcagcagaa cgcggagtcg cagcggcaca acggaatccc cgcactggct
     6001 cagtcgcccg tgttgaccac ggccatcttc ggcctgggtg gacacgacct gcagccgcgc
     6061 cacctgattg ccgcgttcaa ggcgctggat gcaggcaccc ttcctccgct catctatctc
     6121 gggtcgcagt tcttcgacaa gaaccccgac ccgcacatgg cgcagctcca ggagcggctg
     6181 cgcgcggcga acgcggtgga cttcgacgac ctgatcagcg a
//
LOCUS       DAOPJG010000189         6134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_13765_length_6134_cov_6.767889, whole genome shotgun sequence.
ACCESSION   DAOPJG010000189 DAOPJG010000000
VERSION     DAOPJG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13765_length_6134_cov_6.767889"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..2085)
                     /gene="aspS"
                     /locus_tag="PKU75_14080"
                     /pseudo
     CDS             complement(252..2085)
                     /gene="aspS"
                     /locus_tag="PKU75_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
     gene            complement(2180..3142)
                     /locus_tag="PKU75_14085"
     CDS             complement(2180..3142)
                     /locus_tag="PKU75_14085"
                     /inference="COORDINATES: protein motif:HMM:NF022760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF82054.1"
                     /translation="MDVTTRARRAAGRLKHALAARIAEPEAAAHASVGEDAPPRFYGL
                     TRFSVYSPDSGSWKLTRDQGGSYAEQLYAAERMGPRADIFLELAAPILQVMADKHDYR
                     HFVHYSSVMPDPWQSRLRETARRYPVLRLVDAEKVSAVRVMMRRDLIETGAPDRTVVL
                     FRIDDDDILAASYLDKLSAYAVPGDRGRAVSLCRGAGAIYLDGRIGPLRDQQRVFGAQ
                     GQAYVGSWRAADKTLTMTIGRNHTKVARMMPTIADAREVAWLQLRHPAQDMLRDQSDA
                     VAQVQEALAKLAALPPEFDLAADFPTVVSRIDPALAPPAPGDNS"
     gene            complement(3148..3819)
                     /locus_tag="PKU75_14090"
     CDS             complement(3148..3819)
                     /locus_tag="PKU75_14090"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPF82055.1"
                     /translation="MNPNEIMTPIADEFAPARVVASETMYEGLVWDVRRDSVDLGHEV
                     VQRDLVDHPGAVAVIALDEEDRLVLIQQYRHPIRMREWEVPAGLLDVPDEPALLAAQR
                     ELAEEADLHADSWAVLLDYATSPGFTNETIRIFLARGIRPVPEEERHERTGEERDMPV
                     RWVSLDEARDAVLAGRLHNPHTVIAILAAHAMRERGWRDLRPADEPWALRQALRDAAS
                     RAGSA"
     gene            complement(3859..5541)
                     /locus_tag="PKU75_14095"
     CDS             complement(3859..5541)
                     /locus_tag="PKU75_14095"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPF82056.1"
                     /translation="MATTTKHIFVTGGVASSLGKGLTASSLGNLLRARGLRVTMQKLD
                     PYLNVDPGTMNPFQHGEVFVTEDGAETDLDIGHYERFLDVNLAQNANVTTGQVYSQVI
                     AKERRGEYLGDTVQVIPHITNEIKARMRAQAEGDDAPDVIITEIGGTVGDIESLPFLE
                     AARQVRHDLGRDNSMFLHVSLVPYLAPSGELKTKPTQHSVAAMRQVGLQPDALVLRAD
                     RDIPEGIKRKISLMCDVDMDAVAACVDAPSIYDIPKVLHREGLDAFVIRRLGLNFRDV
                     GWDEWDDLLRRVHHPAHQVEVALVGKYIDLPDAYLSVTEALRAGGFAHDARVSIRWVA
                     SDDCETPSGASKALGGVDAVLVPGGFGVRGIEGKVGALRWARENGIPTLGLCLGLQCM
                     VIEYARNVAGIEGASSSEFDPQTPAPVIATMAEQLAFVEGAGDLGGTMRLGTYPARLL
                     EGSVVAGAYGATEVDERHRHRYEVNNSYRDAIGEAGLVFSGTSPDGNLVEFVELPADV
                     HPYYVATQAHPEFKSRPHRAHPLFAGLIGAAMEQQRSSRLVEVDPPVPAAVP"
     gene            complement(5553..>6134)
                     /locus_tag="PKU75_14100"
     CDS             complement(5553..>6134)
                     /locus_tag="PKU75_14100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPF82057.1"
                     /translation="ERAAARERVAAAYGLTRGEPLILTISRVAPQKDLPTLLDAASTL
                     SVPGAWLVVGGGIPHLLSELRARVEREHLPVRFLGPVSDPGDLLLAADLFVLTSRWEA
                     RALVVQEAMAGGIPVVAPNVGGLTDLLAEGGGRLVSPGSAGQIASAADYFLSDAAASA
                     AAADRGRAVAAGWPSVADTTRAWSAAYDALILG"
BASE COUNT          911 a         2141 c         2151 g          931 t
ORIGIN      
        1 gcttgccgcg gcgactcggt ctcggggtcg aactcgccct tgatgatgct ccaggcccgc
       61 tcgtccttgc gcgcccacaa gggtccaccc atgtgaccga tgagcacacg gaccacaccg
      121 tcggctccga catggatggg caggatgccc gcgctggtgc gcgctctggc cggactcatg
      181 ctgcagccat cctttcgatc gcgtccgccg cccgaccggc gggtggccgg cgggcggcat
      241 acggcggaag ctcaggcctg cggcgccggc ccggacgctg cctcgccggg gccggcctcg
      301 cgcgggttgg cgtcggacgg cttggcgtcg atgccggcct ccttgcgctg ctccggcgtg
      361 atcggcgcgg gcgcgtcggt gagcgggtcg tagccaccgc ccgacttggg gaaggcgatg
      421 acatcgcgga tcgagtcgta accaccgagc agcatcacga tgcggtccca gccgaaggcg
      481 atgccgccgt gcgggggcgc cccgaacttg aacgcatcga gcaggaagcc gaacttctcc
      541 tgcgcctgct ccggcgtgag ccccatgatc gcgaagactc gctcctgaat gtccctgcgg
      601 tggatacgga tcgacccgcc accgatctcg ttgccgttgc agaccatgtc gtaggcatag
      661 gccagcgcgg acccggggtc ggactcgagg gtgtcgagga actgctcctg cggggaggtg
      721 aaggcgtggt gcacggccgt ccaggcaccg ccaccgacgg cgacgtcacc ggcagcaaca
      781 gcctccgagg caggctcgaa gagcggcgcg tcgagcaccc acagcagcga ccacgcgtcc
      841 tcgtcgatga gcccgcaccg cttgccgatc tccagccgcg tggcgccgag caacgaccgc
      901 gacggcttgg cctcgcccgc agcgaagaag atgcagtcgc ccggctcggc cccgacgtgg
      961 ccggccagcc caccggtctc ggcctcggag aggttcttgg cgaccggacc ggtcagcgaa
     1021 ccgtcttctt gcacaaggac atacgccaat cccttggcac cccgctgctt ggcgaactcc
     1081 tgccacgcgt ccagggtctt gcggggctga gacgccccgc cgggcatgac cacggcaccg
     1141 acatacggcg cctggaagac ccggaacgtc gtgtcggcga agaagtcggt gcagtcggtc
     1201 agctcctgac cgaaacgcag gtcgggcttg tccgtgccga agcgagccat cgcctcggca
     1261 tacggcatcc gcaggaaggg tgtctccagc tcgaccccga tgagtgacca gatctccttg
     1321 gcgagcgcct cgccgagttc gatgatgtcg tcctgggtga cgaaggacat ctcgatgtcg
     1381 agctgggtga actccggctg ccggtcggcg gaagtcctcg tcgcggtagc agcgcgcgat
     1441 ctggtagtag cgctccatgc cggccaccat gagcagctgc ttgaacagct gcggcgactg
     1501 cggcagggca taccaggagc cgggcgcgag gcgcgccggc acgaggaagt cgcgcgcccc
     1561 ttcgggggtg gatcgggtca gcgtcggcgt ctcgatctcg acgaagtcgc gctcccccaa
     1621 cacctttcgc gctgccgcgt tgacgcgcga gcgcagccgg agcgcgtgac cggcgctgtg
     1681 ggtgccgggc cggcgcaggt cgaggtagcg gtgcttgagg cgcgcctcct cgccgaccgt
     1741 gacgcgctcg tcgatctgga acggcaggac ctcgctcggg ctgagcacct cgatggccga
     1801 ggcgacgacc tcgatctcgc cagtgggcag gtcggggttg acgttgtgct ccccacgagc
     1861 gatgaccgtg ccggtgacct tgacgcagaa ctcgttgcgc agatcgtgcg cggcacccgt
     1921 gagcacctcg tcgcgcgcca cgacctggac cacgccggag gcgtcgcgca gatcgatgaa
     1981 ggcgactccg ccgtgatcgc gccgcttggc cacccagccg gtgagggtga cggtctggtc
     2041 gatctgctcg gcgcgcagcg cgccggcatg gtgggtgcga agcacagggg ttccttcgtc
     2101 ggtggatggg gtggtatgcc gtggcgtgcg tcatcacgcc gttcggctgg tccagtttac
     2161 ggatccgccc gcgggcggcc taactgttat cgcccggcgc cggtggcgcc agcgcgggat
     2221 cgatccgaga gaccaccgtc gggaagtccg ccgcgaggtc gaactccggt ggcagcgcag
     2281 ccagtttcgc cagcgcttcc tggacctggg cgacggcgtc gctctggtcg cggagcatgt
     2341 cctgggccgg gtgtcgcaac tgcagccagg ccacctcgcg ggcgtcggcg atcgtcggca
     2401 tcatgcgggc gaccttggtg tgattgcgcc cgatcgtcat cgtcaaggtc ttgtccgcgg
     2461 cgcgccagct gcccacatac gcctgcccct gcgctccgaa cacccgttgc tggtcacgca
     2521 gcggcccgat gcgcccgtcg agatagatgg cacccgcgcc acggcacaga ctgacggcgc
     2581 gaccccggtc tcccggcacg gcatacgccg acagtttgtc gagatagctc gcggcgagga
     2641 tgtcgtcgtc gtcgatccgg aagagcacca ccgtgcggtc cggggcaccg gtctcgatca
     2701 ggtcgcggcg catcatcacc cgcaccgcgc tgaccttttc ggcatcgacg agccggagca
     2761 cgggataccg gcgggcggtc tcgcgcagcc ggctctgcca cggatccggc atcacgctcg
     2821 agtagtgcac gaagtggcgg taatcgtgct tgtccgccat gacctgcagg atcggcgcgg
     2881 ccagttccag gaagatgtcc gcgcgcgggc ccatccgctc cgccgcatac agctgctcgg
     2941 cgtagctgcc gccctggtcg cgggtcaact tccacgatcc gctgtcgggg ctgtagacgc
     3001 tgaaccgggt cagcccgtag aagcgcggcg gcgcgtcctc gccgacgctc gcgtgcgccg
     3061 cggcttccgg ctcggcgatg cgggcggcga gggcatgctt gaggcgtccg gccgcgcgcc
     3121 gggcgcgggt agtgacgtcc atggcgctca cgctgagccc gcacgcgagg ctgcgtcgcg
     3181 cagggcctga cgcagcgccc agggttcgtc agcgggccgc aggtcgcgcc agccccgctc
     3241 gcgcatggcg tgcgcggcca aaatggcgat gacggtatgc ggattgtgca gccggccggc
     3301 gagcaccgca tcgcgcgcct cgtccagact cacccaccgc accggcatgt cgcgctcctc
     3361 ccccgtgcgc tcgtgccgct cctcctcggg aaccggccgt atgccgcgcg cgaggaagat
     3421 gcggatcgtc tcgttcgtga agccgggcga ggtcgcatag tcgagcagga ccgcccacga
     3481 gtcggcgtgg aggtcggcct cctccgccag ttcacgttgc gcggcaagga gagccggctc
     3541 gtccggcacg tccaggaggc cggccgggac ctcccactcc cgcatccgga tcgggtgccg
     3601 gtactgctgg atcaggacga gccggtcctc ctcgtcgagg gcgatgaccg cgacagcgcc
     3661 gggatgatcg acgagatcac gctgcaccac ctcgtgcccg aggtcgacgc tgtcgcggcg
     3721 cacatcccag accaggccct catacatcgt ctcggaggcc acgacccggg cgggggcgaa
     3781 ctcgtcggcg atgggcgtca tgatctcgtt cgggttcacg cgggccattg tcccagcccg
     3841 ccgcgtccgg gaacgcgctc aggggaccgc ggccgggacc ggcgggtcga cctcgaccag
     3901 ccgcgaggag cgttgctgct ccatggccgc gccgatgagt ccggcaaaaa gcgggtgggc
     3961 ccggtgcggc cgcgacttga actccggatg cgcctgggtc gcaacgtaat aggggtgcac
     4021 atcggcgggc aactcgacga actccaccag gttgccgtcc ggtgaggtgc cgctgaagac
     4081 gagccccgcc tccccgatcg cgtcgcggta ggagttgttg acctcgtacc ggtgccggtg
     4141 ccgctcgtcg acctcggtcg cgccataggc tcccgccacg acggacccct ccagcaggcg
     4201 cgccggatag gtgcccaggc gcatcgtgcc gccgaggtca ccggcgccct cgacgaaggc
     4261 cagttgttcg gccatcgtcg cgatgaccgg ggccggcgtc tgcgggtcga actcgctcga
     4321 ggacgccccc tcgatgccgg cgacattgcg ggcgtattcg atgaccatgc actgcaaccc
     4381 caggcagagc ccgagagtcg gtatgccgtt ctcccgggcc caccgcaggg cgcccacctt
     4441 gccctcgatg ccgcggacgc cgaagccgcc tggcacgagc accgcgtcga caccgccgag
     4501 cgccttggag gcgccggacg gagtctcgca gtcgtcggac gcgacccacc ggatgctcac
     4561 tcgcgcgtcg tgggcgaagc cgccggcgcg tagcgcctcg gtcaccgaca gataggcgtc
     4621 gggcaggtcg atgtacttgc cgaccagcgc cacctcgacc tggtgggccg gatggtgcac
     4681 gcggcgcagc aggtcgtccc actcgtccca gccgacatcg cggaagttca gcccgagccg
     4741 ccggatgacg aaggcgtcca agccttcgcg gtgcagcacc ttggggatgt catagattga
     4801 gggcgcgtcc acgcatgcgg cgacggcgtc catgtcgaca tcgcacatga gcgagatctt
     4861 gcgcttgatg ccctccggaa tgtcccgatc cgcccgcagc acaagggcgt ccggctgcag
     4921 accgacctgc cgcatcgccg cgacggagtg ctgggtcggc ttcgtcttca gctcaccgct
     4981 cggggccaga taggggacga gcgagacgtg caggaacatc gagttgtcgc ggccgaggtc
     5041 gtggcggacc tgccgggcgg cctcgaggaa gggcagcgac tcgatgtcgc cgacggtgcc
     5101 cccgatctcg gtgatgatca cgtcgggcgc gtcgtcgcct tcggcctgcg cccgcatgcg
     5161 ggccttgatc tcgttggtga tgtgcgggat gacctgcacc gtgtcgccga gatattcgcc
     5221 ccggcgctcc ttggcgatga cctggctgta gacctgcccc gtggtgacat tggcgttctg
     5281 cgcgaggttg acgtcgagga agcgctcgta gtggccgatg tcgaggtcgg tctccgcgcc
     5341 gtcctcggtg acgaagacct cgccgtgctg gaacggattc atcgtcccgg gatcgacgtt
     5401 gagatagggg tcgagcttct gcatcgtcac ccgcaacccg cgtgcgcgga ggaggttgcc
     5461 gaggctcgag gccgtcagcc cctttccgag ggaggaggcc acgcctccgg tcacgaagat
     5521 gtgtttcgtc gtggtcgcca cggggttcta ggttatccca ggatcagggc gtcgtatgcc
     5581 gctgaccacg cccgggtcgt gtcggcgacg ctcggccacc cggccgcgac tgcccgcccg
     5641 cggtccgcgg ccgccgcaga cgccgccgcg tccgacagga aatagtctgc ggcagaagcg
     5701 atttgaccgg cagagccagg tgacacgagc cgaccgccgc cctcggccag gagatcggtc
     5761 aacccgccga cattcggcgc caccaccggt atgccgcccg ccatggcctc ctgcaccacc
     5821 agcgcgcgcg cctcccatcg gctcgtcagg acgaacaggt cagcggccag cagcaggtcg
     5881 cccgggtccg agaccgggcc gaggaagcgc accggcaggt gctcgcgctc cacacgagcg
     5941 cgtaactcgg acagcaggtg cggtatgccg ccgcccacga cgagccaggc ccccggcacg
     6001 ctcagggtgg aagccgcgtc gagcagcgtg ggcaggtcct tctggggggc gacccgagag
     6061 atggtgagga tgagtggctc cccgcgcgtc agcccgtatg ccgcagccac ccgctcccgc
     6121 gccgctgccc gttc
//
LOCUS       DAOPJG010000190         6121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_13805_length_6121_cov_6.617046, whole genome shotgun sequence.
ACCESSION   DAOPJG010000190 DAOPJG010000000
VERSION     DAOPJG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13805_length_6121_cov_6.617046"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKU75_14105"
     CDS             <1..678
                     /locus_tag="PKU75_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HPF82058.1"
                     /translation="VPIAQRYGAWVHVDAAMAGSAMILPEMRHLFAGIDGADSIVWNP
                     HKWLGTILDCSLLYVRDVDHLVACMSTTPSYLRSSADDQVVQYKDWGIPLGRRFRALK
                     LWFHLRLEGVEAIRERLRRDLANAQWLAETVAAEPDWRVLAPVDLQTVCLRHEPAGLD
                     GEALDRHTLAWADALNASGTAFVSPSKLDGRWMVRVSIGAEATERSDVEGLWAALRAN
                     ADAAGTQ"
     gene            complement(791..1840)
                     /locus_tag="PKU75_14110"
     CDS             complement(791..1840)
                     /locus_tag="PKU75_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82059.1"
                     /translation="MHARRLTLGTISAAAALASIAAGGSATAASGSSADRTAAVGHSV
                     SSAEQRATARLDVEAIVKSAKPMQLPAGRGGAARPSSLERGVETKVGPVAVKAGATER
                     AVNGVPDSYAGSLWPLGKNLNPNRQTGKYYFNTGSGWSWCTATVVTAANRSTLVTAGH
                     CVVNPSTKQWYTNAFFRPGYQYGNTLGTWYARNMWTTGNYYYYGSYADDMAAVVVRTN
                     GAGTPIQAITGSQGIWFNGPQGGLMRTTLGYPVTDSRWPGYTANGEDARYCQWTNTYY
                     ATGTWAGQEYIPCYMTGGSSGGGHLSWVNTNWMGYVNSVNSNKGGIGSSWAHVMFSPY
                     FGTNEANVYNAAKAA"
     gene            2029..2343
                     /locus_tag="PKU75_14115"
     CDS             2029..2343
                     /locus_tag="PKU75_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82060.1"
                     /translation="MSIGAASRLATQPSAVVDISGGNDTTPADAAELTSPLLLIGSAD
                     DSSTPMTRLREIEKAATNASSVTVREVPGDAHASAIFDGPSGESVLNDIVSFLDEHAA
                     AN"
     gene            2407..4485
                     /locus_tag="PKU75_14120"
     CDS             2407..4485
                     /locus_tag="PKU75_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPF82061.1"
                     /translation="MQQQAPHRPAWSAALFSVVVMIACLGASAAPMGAAVTPRVSAVV
                     PASGPPRGGATITIQGSGFTGVERVLFGTIAARSVKVLSPRSIQVLTPAHAAGSVHVK
                     VVTGGGSSPATAQDRYAYVGPPAVTGLAPAIGSTAGGTVVQVTGERFLGVRQVLLGEQ
                     PAHSVTVVDSRTLRATAPAAAAGDVDVRITTFSGTSPVVSAGVFRYEDPPAISHLTPA
                     AGPSSGGTEVTITGTGFVGTTAVTFGGIPATWTLTSPTTLVATSPASTRAGTVDVQVQ
                     AGGLISAAGAAASQFTYDWVGPTITGLDPRQGPMAGGTPITLTGTGFTGVTGVLFGEA
                     TASYSVVSDTEISAVTTPGLTPGPVRVQVTTSSGTTPEDPHAVYTYEGGGPAGGNGPL
                     LSYTRGVDSDRDGTFQGGSAEELTTDVFIQNVAEGTAPVMVNPPNREADITPSFSPDG
                     TKIAWASSATNNGTFDIFVKDLSTGVITNLTNTGAGQFERWPNWSVDGAQIVYSKRTR
                     ANNLDIWIMNADGSDQHYVAGQVGPGQTAEDCCASFDLLGGVVFASNRAGNFDVYRYG
                     LEPRSATEDPARLTRLTRSTSYEGTPSVEATGTVVYRHGTEQQVYRVDPAQPGAPPMR
                     VPTPGLIRTPSGTPDGRGLVFGWRERPGPPLDIAWAAADGTGLVMLTDTPTLTETDPA
                     WQPAPVSPSD"
     gene            4610..4879
                     /gene="rpsO"
                     /locus_tag="PKU75_14125"
     CDS             4610..4879
                     /gene="rpsO"
                     /locus_tag="PKU75_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPF82062.1"
                     /translation="MPLSTDVKKQIIAEYGQSESDTGSPEVQIALLTQRIKDLTEHSR
                     THKHDHHSRRGLLLLVGQRRRLLRYLESVDVERYRALIKRLGLRR"
     gene            5060..>6121
                     /locus_tag="PKU75_14130"
     CDS             5060..>6121
                     /locus_tag="PKU75_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPF82063.1"
                     /translation="MEGPEIQFAEAVIDNGSFGTRTVRFETGRLARQAGGSVTAYLDG
                     ETTVLSTTTAGKTPKDQFDFFPLTVDVEERMYALGKIPGSFFRREGRPSTEAILTCRL
                     IDRPLRPSFAKGLRNEVQIVETVLALNPDHQYDVLAINAASASTQISGLPFSGPIGGV
                     RVSLMDGQWVAFPNFSDAERSTFDMVVAGRIVGDDVAIMMVEAEATDATWNLVKNEGK
                     QAPTEEIVAQGLEAAKTFIKALCEAQSQLAAAAAKPVEEFPRFLDYEDDAYAAVEEFV
                     SRDGKLAQILTIAGKQEREDALDELKSAVHLELVGTADVFTGEGEAGPFTGRQKEISA
                     AYRSVQKKLVRERILRDKVR"
BASE COUNT          966 a         2155 c         2085 g          915 t
ORIGIN      
        1 gtgcccatcg cgcagcggta tggcgcgtgg gtgcacgtcg atgccgccat ggcgggctcg
       61 gccatgatcc tgccggagat gcggcacctg ttcgccggca tcgacggcgc cgactccatc
      121 gtgtggaacc cccacaagtg gctcggcacg atcctcgact gctcgctgct ctatgtccga
      181 gacgtcgacc acctcgtcgc ctgcatgtcg acgacgccgt cctatctgcg ctcgtcggcc
      241 gacgaccagg ttgtgcagta caaggactgg ggcatcccgc tcgggcgtcg cttccgggcc
      301 ctcaagctgt ggttccacct gcggctcgag ggcgtcgagg cgatccgcga gcgcctgcgc
      361 cgcgacctcg cgaacgcgca gtggctagcc gaaaccgttg ctgcggagcc ggattggcgg
      421 gtgctggcgc ccgtggacct gcagaccgta tgcctgcgcc acgagcccgc gggcctggac
      481 ggggaggcgt tggaccggca taccctcgcc tgggcggacg cgctcaacgc gagcggcacg
      541 gcgttcgtct ccccgagcaa actcgacggc cggtggatgg tgcgggtctc catcggcgcc
      601 gaggcgaccg aacgatccga cgtcgagggg ctgtgggcgg cgttgcgcgc caacgccgac
      661 gcggccggga cacagtagac acgaccagtg ccggcccacg gattcacctg ggcgttgtcg
      721 gagggggacg tgcggcgccc ccgcagccgg tgatccgagc tgcgggggcg gcaatgggtt
      781 cggctcgccg tcaggcggcc ttggcggcgt tgtagacgtt cgcttcgttg gtgccgaagt
      841 acgggctgaa catcacgtga gcccacgagg agccgatccc gcccttgttg ctgttgacgg
      901 agttgacgta gcccatccag ttggtgttga cccaggacag gtgcccgcca ccgctcgatc
      961 cacccgtcat gtagcagggg atgtactcct ggccggccca ggtgccggtg gcgtagtagg
     1021 tgttggtcca ctggcagtag cgggcgtcct cgccgttggc ggtgtagccg ggccagcgcg
     1081 agtcggtgac cgggtagccg agcgtggtcc gcatgaggcc accctgcggg ccgttgaacc
     1141 agatgccctg ggagccggtg atcgcctgga tcggcgtgcc cgcgccattg gtccggacga
     1201 cgacggcggc catgtcgtcc gcgtaggagc cgtagtagta gtagttcccg gtcgtccaca
     1261 tgttccgcgc gtaccaggtg ccgagggtgt tgccgtactg gtagccgggg cggaagaagg
     1321 cgttggtgta ccactgcttc gtcgacgggt tgacgacaca gtgtcccgcc gtgaccagtg
     1381 tcgaccggtt ggccgcggtg acgaccgtgg cggtgcacca gctccagccg ctgccggtgt
     1441 tgaagtagta cttgccggtc tgccggttcg ggttgaggtt cttgccgagc ggccagagcg
     1501 agccggcgta ggagtcgggg acgccgttga cggcgcgctc ggtcgcgccc gccttgacgg
     1561 ccaccggacc gaccttggtc tcgacgccgc gctcgaggct ggagggccgc gcggcgccgc
     1621 cgcgaccggc gggcaactgc atgggcttgg cggacttgac gatcgcctcg acgtcgaggc
     1681 gcgcggttgc ccgctgttcg gcggatgaca cgctgtggcc gaccgcggcc gtgcggtctg
     1741 cactggatcc ggacgcggcg gtggctgatc caccggcggc gatgctggcc agagcggcgg
     1801 cagcgctgat ggtgccgagc gtgagacggc gtgcgtgcat gacaaaactc cctctgcgag
     1861 atgggttccc gggcatgagg acggcgaccc ggtcagcgag acttcgctga cccaatggag
     1921 tgcaccaggc cccggttgat acaccgtcgg tccggctcca gtcactggag tgactcgggg
     1981 cgcccacgtc ctccgccgcc ctggtccggc ggtagcctcg gtggccgcat gtccatcggc
     2041 gcggcgtcgc ggctcgccac ccaaccctcc gccgtcgtgg acatctccgg aggaaatgac
     2101 acgaccccgg ccgatgccgc cgagctgacc agcccgctgc tcctcatcgg ctccgcggac
     2161 gacagctcga ccccgatgac ccggctgcgg gagatcgaga aggctgcgac gaacgcctcg
     2221 tccgtcacgg tgcgggaagt gcccggcgac gcccatgcca gcgccatttt cgacggcccg
     2281 tccggtgagt cggtcttgaa tgacatcgtc tccttccttg acgagcacgc cgcagcgaac
     2341 tgaccgacgc gtgcgtcccg cgcagtccgt ctggaccggc cggcctgcat cgtcgatcct
     2401 tggggcatgc agcaacaggc cccacaccgg ccggcgtgga gcgccgccct gttctccgtc
     2461 gtggtcatga tcgcctgcct cggcgcgtcc gcagccccga tgggagcggc cgtcaccccg
     2521 cgcgtgtccg cggtcgttcc ggcatccggg ccgccacggg gcggggccac gatcaccatt
     2581 cagggcagcg gcttcaccgg cgtcgaacgc gtgctgttcg ggaccatcgc agccaggtcg
     2641 gtgaaggttc tctcgccgag gtccatccag gtcctcaccc ctgcccacgc tgccggttcg
     2701 gtccacgtca aggtcgtgac aggagggggg tcgtcccccg ccaccgccca ggatcggtac
     2761 gcctacgtcg gaccaccagc cgtgaccggg ttggccccgg cgatcggcag caccgcgggc
     2821 gggaccgtcg tccaggtgac cggcgagcga ttcctcggcg tccggcaggt ccttctcggc
     2881 gagcaaccgg cccactcggt cacggtggtc gactctcgca ccctccgcgc cacggcgccg
     2941 gcggcggccg cgggagatgt cgatgtacga atcaccacct tctccggcac ctcaccagtg
     3001 gtctcggccg gcgtgttccg gtatgaggat ccgccggcga tctcgcacct cacgccggca
     3061 gccggcccgt cctcgggagg gaccgaggta acgatcaccg gcaccggatt cgtggggacg
     3121 acggcggtga ccttcggagg aatccccgcg acctggacgc tgacctcacc gacgacgctg
     3181 gttgccacgt cccccgcgag cacccgcgcc ggaacggtgg acgtccaggt gcaggccggg
     3241 ggtctaatct ccgccgccgg cgcggccgcg agccagttca cctatgactg ggtgggaccg
     3301 acgatcaccg ggctcgatcc ccggcaggga cccatggccg ggggcacccc gatcaccctg
     3361 accggaacgg gcttcacagg agtcaccggc gtgcttttcg gggaggcgac cgcgtcctat
     3421 tcggtggtct cggacaccga gatcagcgcg gtgaccactc cgggcctcac ccccggcccg
     3481 gttcgggtgc aggtgacgac gtcgtcgggc acgacgccgg aggacccgca cgccgtctac
     3541 acctatgagg ggggcggccc cgccggcggc aacgggccgt tgctctccta tacccggggt
     3601 gtcgactcgg accgggacgg gacctttcag ggcggaagcg ccgaagaact gaccacggat
     3661 gtcttcatcc agaacgtcgc cgagggcact gccccggtca tggtgaaccc accgaaccgc
     3721 gaggcggaca tcacccccag tttctctccg gacgggacca agatcgcctg ggcctcgtcc
     3781 gcgacgaaca atggcacctt cgacatcttc gtcaaggatc tcagcacggg cgtcatcacc
     3841 aacctgacca ataccggtgc gggacagttc gaacgctggc ccaactggtc tgtcgacggc
     3901 gcacagatcg tgtacagcaa gcggacgcga gccaacaacc tcgacatctg gatcatgaac
     3961 gccgacggga gcgatcagca ctacgtggcc gggcaggtcg ggcccggcca gaccgcggag
     4021 gactgttgcg cgtccttcga cctgctcggc ggggtggtct tcgcgtccaa ccgcgccgga
     4081 aacttcgacg tgtaccggta tgggctcgag ccacgtagtg ccacggagga cccggctcgc
     4141 ctgaccaggc tcacccggtc caccagctac gaggggaccc cgtcggtcga ggccaccggt
     4201 acggtggtgt accggcacgg caccgagcag caggtctatc gcgtcgatcc ggcccaaccc
     4261 ggcgctccgc cgatgcgcgt gcccacgccc ggcctgatcc gcactccgag cggaaccccc
     4321 gacggccggg gcctggtgtt cggctggcgg gagcgtcccg ggccgccgct cgatatcgcc
     4381 tgggcagccg cggacgggac gggtctggtc atgctcacgg acacgccgac gctgaccgaa
     4441 accgatccgg cctggcagcc agcgccggtc agtcccagcg attaggggac aacccgggcc
     4501 cgctggtagc cttgagcggt tgccgtgaca acggccgcgg acccagagag ccccggtgaa
     4561 ccagcccggg tgcgccgcgc aacgacatac gaatggagac cggacgccca tgccactgag
     4621 caccgacgtc aagaagcaga tcatcgccga gtacggccag tccgagagcg acaccggctc
     4681 tcccgaggtg cagatcgcgc tgctcaccca gcggatcaag gacctcaccg agcacagccg
     4741 cacgcacaag cacgaccacc actcccgccg tggtctgctg ctgctcgtcg gtcagcgccg
     4801 ccgcctgctg cgctacctcg agagcgtcga cgtggagcgc taccgtgcgc tgatcaagcg
     4861 cctcggcctg cgccgttgac ggtgcacagc gccgatgaga attgagaaga ggcgacccgc
     4921 aaaccgggcc gcctcttctc gtatgccgta gccacggcat acccgcagct cgcccgggga
     4981 gccgggcgcg ccgcataccg aaagaacccc gtgcacaacg acgatgcacg aggcgaaagc
     5041 tgagagaaag cgagggccca tggagggtcc agaaatccag ttcgcggaag ccgtcatcga
     5101 caatggcagc ttcggcaccc gcacggtccg gttcgagacc ggacgcctcg cccgccaagc
     5161 gggcggttcc gtcaccgcat acctcgatgg cgagaccaca gtcctgtcga ccaccacggc
     5221 cggcaagacc ccgaaggacc agttcgactt cttcccgctg accgtcgacg tcgaggagcg
     5281 catgtacgcc ctcggcaaga tcccgggcag cttcttccgt cgtgagggcc gcccgtccac
     5341 cgaggccatc ctcacctgcc gcctgatcga ccggccgctg cgcccgtcct tcgccaaggg
     5401 cctgcgcaac gaggtccaga tcgtcgagac ggtcctggcc ctcaaccccg accaccagta
     5461 cgacgtgctc gccatcaacg cggccagcgc ctcgacccag atctccggcc tgcccttctc
     5521 cggccccatc ggcggcgtgc gcgtctcgct gatggacggc cagtgggtgg ccttcccgaa
     5581 cttctccgac gccgagcgct cgaccttcga catggtcgtc gccggccgga tcgtcggcga
     5641 cgacgtcgcg atcatgatgg tcgaggccga ggccaccgac gccacctgga acctcgtcaa
     5701 gaacgagggc aagcaggctc cgaccgagga gatcgtcgcc cagggcctgg aggccgccaa
     5761 gaccttcatc aaggcgctct gcgaggcgca gagccagctc gccgctgcgg cggcgaagcc
     5821 ggtggaggag ttcccgcgct tcctcgacta cgaggacgat gcgtatgccg ccgtcgagga
     5881 gttcgtctcc cgcgacggca agctcgccca gatcctcacg atcgccggca agcaggagcg
     5941 cgaggacgcc ctggacgagt tgaagtccgc cgtccacctc gaactcgtcg gcaccgccga
     6001 cgtcttcacc ggtgagggtg aggccggacc gttcacgggc cggcagaagg agatcagcgc
     6061 ggcctaccgc tcggtgcaga agaagctcgt gcgcgagcgc atcctgcgcg acaaggtccg
     6121 c
//
LOCUS       DAOPJG010000191         6098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_13872_length_6098_cov_6.971703, whole genome shotgun sequence.
ACCESSION   DAOPJG010000191 DAOPJG010000000
VERSION     DAOPJG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13872_length_6098_cov_6.971703"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1009)
                     /locus_tag="PKU75_14135"
     CDS             complement(218..1009)
                     /locus_tag="PKU75_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HPF82064.1"
                     /translation="MKVAVIQLAYDDAEPIEERRERVAELVRMQRGHDLVVLPELWGA
                     TGFDYQKWEASAEPLYGPTVELLAGVARDIGSMVHLGSIIERLPEPGVEGRHLANTSV
                     VLSPAGEPVAVYRKIHRFGFGAGEPKLLEAGGEIVTVGLPGGHTAGLSTCYDLRFPEL
                     YRRQLDAGAEVFLVPAAWPKRRVEHWSLLGRARAVENQCAFIACNTAGTHGGYEMGGH
                     SQVVAATGEVLAEAGLDEAVLSVDLDLGAVARWREEFPVLADRRL"
     gene            complement(1018..3030)
                     /locus_tag="PKU75_14140"
     CDS             complement(1018..3030)
                     /locus_tag="PKU75_14140"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HPF82065.1"
                     /translation="MTSSPPQGLDRRLGLAVLLPALVVGIVGAALSGAVVAYVIGDPG
                     PVMRWTALISRILRDVAATATIGFLLVGAFLAPETRRTSRRAQLTRLAAASGSLWLVT
                     LVVSVVAEFADISGLAPTQPNFWAQFFGLTWELSTTRMTVLAAIFVAILVVICSAPRG
                     TTGLAWAFVLGWLALMPQALAGHASVAGDHMSAVNGLAVHLVAVTTWAGGLLAILVMR
                     RSLHPHLAVVVRRFSTIAVWSYAALALSGLLIAWIGMAGLGDLRSGYGALLLIKVGAL
                     VVLGYVGWLHRRGMIARLEKDDGDSAAFVRLAVGELLLMGVALGAAVALARTPPPSSD
                     VLSPDPTSVYELTGYPDPGPVPDRAWISVWHNDWLWIAVAAVAVFVYLRWVHRLHKRG
                     DRWPLWQSLIWVLGWAIFVYSMCGVTGVYGRIMFSWHMIMHMTVAMLVPLLLVPAAPI
                     TLALRALPARHDKTMGPREFILQLVHSRYLRVVANPVVAAVIFFFSLATFYFTPLFYY
                     ALATHTGHVLMTVHFLASGYLFAWVLVGTDPGPRRWPPLVLLVVLFATISFHAFLGVV
                     ITDSHALLAPEFFTRLGLGWLPDPLEDQHKAGAIAWGIGEAPTLALAIMVTIQWLRQD
                     RRETERWDRQAERDHDAELAAYNARLAKIAEHDRELAARDKTHQRH"
     gene            3211..4956
                     /locus_tag="PKU75_14145"
     CDS             3211..4956
                     /locus_tag="PKU75_14145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPF82066.1"
                     /translation="MSKDFQHLSPAFPGRAPWGTASSLRAWQAAALEQYLREEPRDFL
                     AVATPGAGKTTFALRVARELLDRRVVNKITIVAPTEHLKVQWADAAHKAGINIDPGLG
                     GSRRGRSKQFVGTAVTYAGVAARHFHYRALTENSNSLVILDEIHHAGDARTWGEAVAE
                     CFEPARRRLALTGTPFRSDDNPIPFVRYEELPDGSKRSAADYTYGYADALRDHVVRPV
                     LFMAYGGPMRWRTKAGEELEADLAAPLTKDLTAHAWRTALDPKGEWIQSVLRAADTRL
                     TEVRRHIPDAGGLVIATDQAQARAYARLLHELTGDKPTVVLSDDAGASQKIEEFSESD
                     ARWLVAVRMVSEGVDVPRLAVGVYATATSTPLFFAQAVGRFVRARRRGEVATIFLPTV
                     APLLSHATLLERQRDHILGAAKGEDWSEEDGLLAAAQREESAADDLLGEWEALGSNAH
                     FDHVLFDAHQFGLQSAVGSQEEADYLGLPGLLEPDQVAEVLRERQRKHAARHTRRERR
                     EKVSPEVSLHRALEAQRKLLNSLVSAYAHHRGVPHSHVHAELRRQAGGPSLAKATTEQ
                     VHERIALIRRWHTGK"
     gene            5107..>6098
                     /locus_tag="PKU75_14150"
     CDS             5107..>6098
                     /locus_tag="PKU75_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82067.1"
                     /translation="MNPKKLLATLGAGVLALCAVAPSATAEPAAGSKANGSSTAPDVT
                     TNGRHVVFSSVATDLVTGDTNGRSDIFLRDIAFKKTKIVTKSYTGGPANGNSTEPQVS
                     DDGRYVVYMSDATNLVPGDTNGKTDIFRTDMTTMKTTRISTSQSGAQANGVSKRPRIN
                     SAATVIAYVSTAPNLVPGDTNRIQDIFVRNLNNNSVKRISVSPTGAQLSVMPFSPSVS
                     YDGKIVGFHTDAYANPTVNSYCGRTADALSVIMKWTVSTSKSSITEVVCRQSGVRRQV
                     YEPRANMQNKVGYAVASDWGYTEFFARGLYTLYDSIVEGSSYSTPYWDVARWGGT"
BASE COUNT         1077 a         2164 c         2033 g          824 t
ORIGIN      
        1 ccgcaccgtc tcgcacgtcg accccaacac gggcacgcgc gtggaagagc accgggtcga
       61 cggcctctga cccacccgat ctggaccgcg tcgacgcaat ccgattgccc cacagcgaca
      121 tagcagaggt taatcaaatc agcacagcgt gctaggtgtg ttgatttcgc caacctctgc
      181 taacagaggt tccttggggc ccgcgcgcag cgcggcgtca aaggcgccgg tcggccagga
      241 ccgggaactc ttcgcgccac cgggcgaccg ccccgaggtc gaggtcgacg ctgagcaccg
      301 cttcgtccag gcccgcctcg gcgagcacct cccccgtggc ggcgaccacc tgggagtggc
      361 cacccatctc gtatccgccg tgcgtgccgg cggtgttgca cgcaatgaag gcgcactggt
      421 tctcgacggc ccgggcccga cccagcaggc tccaatgctc gacacgtcgc ttcggccatg
      481 ccgcgggcac gaggaagacc tcggccccgg cgtcgagttg ccggcgatag agctcgggga
      541 agcgcaggtc gtagcaggtc gacaagccgg ccgtatgccc gccgggcagg ccgaccgtca
      601 cgatctcgcc gccggcctcc agcagcttgg gctcaccggc gccgaagccg aagcggtgga
      661 tcttgcggta gacggcaacg ggctcaccgg caggcgagag aacgacggac gtattcgcca
      721 ggtgccggcc ctcgaccccg ggctcgggca gcctctcgat gatcgagccc aggtgcacca
      781 tcgacccgat gtcgcgggcc acccccgcga ggagctcgac cgtcggccca tacagcggct
      841 cggcgctcgc ctcccacttc tggtagtcga aacccgttgc gccccacagc tccgggagca
      901 ccacgaggtc gtggccccgc tgcatacgca ccagctcggc gacccgttca cgccgctcct
      961 caatgggctc ggcgtcgtca taagcgagtt ggatgacggc gaccttcatg gttctcctca
     1021 gtgccgttgg tgggtcttgt cgcgggccgc gagttcgcgg tcgtgctcgg cgatcttggc
     1081 gagccgggcg ttgtatgccg ccagctcggc atcgtggtcg cgctcggcct gccggtccca
     1141 gcgctcggtc tcgcggcggt cctgacgcag ccactggatg gtgaccatga tcgcgagggc
     1201 gagggtgggc gcctcaccga tgccccacgc gatggcaccg gccttgtgct gatcctctag
     1261 tgggtcgggc agccagccca ggccgagccg ggtgaagaac tccggcgcca acagcgcatg
     1321 actgtcggtg atgacgacac cgaggaaggc gtggaaggag atcgtcgcga agagcacgac
     1381 gagcagcacc agcggcggcc agcggcgcgg gcccggatcg gtgccgacga gcacccaggc
     1441 gaagagatag cccgacgcca ggaagtgcac ggtcatcagc acatgcccgg tgtgcgtggc
     1501 caaggcgtaa tagaagagcg gcgtgaaata gaaggtcgcc aggctgaaga agaagatgac
     1561 ggccgcgacg accggattgg ccacgacccg cagatagcgc gagtgcacca gctggaggat
     1621 gaactcgcgt gggcccatcg tcttgtcgtg ccgggccggc agcgcccgca gcgcgagggt
     1681 gatcggtgcg gccgggacga gcagcagcgg gacgagcatc gccacggtca tgtgcatgat
     1741 catgtgccag gagaacatga ttcgcccgta gaccccggtc acaccgcaca tcgagtagac
     1801 gaagatcgcc caccccagca cccagatcag gctctgccac aagggccacc tgtcaccccg
     1861 cttgtgcagg cggtgcaccc agcgcagata gacgaagacg gccaccgccg ccacggcgat
     1921 ccacagccag tcgttgtgcc agacactgat ccaggcccgg tccgggaccg gccccggatc
     1981 gggatagccg gtcagttcgt agacgctggt cggatcgggc gagagcacgt cggacgacgg
     2041 cggtggtgtg cgggccaggg cgacggcggc accgagcgcc acccccatga gcagcagctc
     2101 gcccacggcg agccgcacga aggccgcgct gtcgccgtca tccttctcga gccgcgcgat
     2161 catcccgcgg cgatgcagcc agccgacata gccgaggacc acgagggccc cgaccttgat
     2221 gagcagcaac gcgccatacc cgctgcgcag gtcacccaag ccggccatcc cgatccaggc
     2281 gatcagcaga ccgctcaggg cgagcgcggc atacgaccag acggcgatcg tggagaagcg
     2341 ccgcacgacg acagccaggt gcgggtgcag cgaacggcgc atcaccagga tcgccaacag
     2401 acccccggcc caggtcgtca cggcgaccag gtgcaccgcc aacccgttga cggcgctcat
     2461 gtggtcgccc gcgacggagg cgtggccggc gagcgcctgg ggcatcagcg cgagccagcc
     2521 gaggacgaag gcccaggcca gcccggtcgt gcctcgcggg gcgctgcaga tgacgacgag
     2581 gatcgcgacg aagatcgcgg ccagcacggt cattcgggtc gtcgacagct cccaggtcaa
     2641 cccgaagaac tgggcccaga agttcggttg cgtcggcgcc agcccgctga tgtcggcgaa
     2701 ttccgcgacc acgctgacga cgagcgtgac cagccacaga ctgccggagg cggcggccag
     2761 ccgggtcaac tgcgcccgcc gggacgtccg gcgcgtttcg ggggcgagaa aggccccgac
     2821 cagcaggaat ccgatcgttg ccgtggcggc gacatcccgc aggatgcggc tgatcagggc
     2881 ggtccagcgc atcaccggcc ccggatcgcc gatgacatag gcgacgaccg cccccgacag
     2941 cgccgccccc acgatcccga ccaccagggc ggggaggagc acagccagac cgagtcgccg
     3001 gtccagcccc tggggcgggg aggacgtcat accgtccagc ctagatcgcg gccccgacaa
     3061 cgactgtggc acctcccact gcgatgcgcc ggatgaggca agatagtcag tccgtgcccg
     3121 ccgcccggac caccgaaatg gacagatgag cgctacccca cgaagctctt tccccgcttc
     3181 gccccgccag ctgcttcgcg aggtccccgc gtgagcaagg acttccagca cctctccccc
     3241 gccttcccgg ggcgcgcgcc gtggggcacg gcgtccagcc tgcgcgcctg gcaggccgcg
     3301 gcgctggagc agtacctgcg cgaggagccg agggacttcc tcgccgtcgc gacgcccggc
     3361 gccggcaaga cgaccttcgc cctgcgggtc gcccgcgaac tgctcgaccg gcgggtcgtc
     3421 aacaagatca cgatcgtcgc gcctaccgag catctgaagg tccaatgggc cgacgccgcc
     3481 cacaaggccg gcatcaacat cgaccccggg ctgggaggct cgcgccgggg ccgcagcaag
     3541 cagttcgtgg gcaccgccgt gacgtatgcc ggtgtcgcag cgcgccattt ccactatcgc
     3601 gcgctgaccg agaactccaa ctccctcgtc atcctcgacg agatccacca cgccggcgac
     3661 gcccgcacgt ggggcgaagc agtggccgag tgcttcgaac cggcccggcg gcggctggcg
     3721 ctgacgggca cgcctttccg ctccgacgac aacccgatcc cgttcgtccg gtatgaggag
     3781 ttgccagacg ggagcaagcg ctcggctgcc gactacacct acgggtatgc cgatgccttg
     3841 cgtgatcacg tcgtgcggcc ggtgttgttc atggcctatg gcgggccgat gcgttggcgc
     3901 accaaggccg gcgaggagtt ggaggccgat ctcgccgctc ccctgacgaa ggacctgacc
     3961 gcccacgcct ggcgcaccgc cctcgacccc aagggcgagt ggatccagtc ggtgctgcgc
     4021 gcggccgaca cccggctgac cgaggtgcgc cgccacatcc ccgacgccgg cggtctggtg
     4081 atcgccaccg accaggcgca ggcacgggca tacgcccggc tcctgcacga actgaccggc
     4141 gacaaaccga ccgtcgtgct ctccgacgac gcaggcgcgt cgcagaagat cgaggagttc
     4201 agcgagtccg acgcgcgctg gctcgttgcc gtgcgcatgg tctccgaagg ggtggatgtg
     4261 ccgcggctgg cggtcggggt ctatgcgacg gcgacgtcca cgccattgtt cttcgcgcag
     4321 gcggtgggcc ggttcgtgcg agcgcggcgg cgcggggagg tggcgacgat cttcctgccg
     4381 accgtggcgc cgctgctctc gcacgccacc ttgctggagc gccagcgcga ccacatcctc
     4441 ggcgccgcga agggcgagga ctggtcggag gaggacgggc tgctcgccgc ggcgcagcgc
     4501 gaggagtcgg cggccgacga cctcctcggc gagtgggagg cgctgggctc caatgcccac
     4561 ttcgaccatg tcctcttcga cgcccaccag ttcggcctgc agagcgctgt cgggtcgcag
     4621 gaggaggccg actacctcgg gctgccgggg ctgctcgaac cggaccaggt tgccgaagtg
     4681 ctccgcgagc ggcagcgcaa gcacgccgcg cggcataccc gccgcgagcg acgagagaag
     4741 gtgagccctg aggtctcgct gcaccgcgcg ctcgaggccc agcgcaagct gctcaactcc
     4801 cttgtcagcg cctacgccca ccaccgcggc gtgccgcact cccacgtcca cgcggagttg
     4861 cgccggcagg ccggtggtcc ttcgctggcg aaggcgacca ccgagcaggt ccacgagcgc
     4921 atcgccctca ttcgccgctg gcataccggc aagtaacaac tcgcgacgaa aaaaattgcg
     4981 gcacacggta gttggctggc gaccatcgcg ccactcgagt tggtcctaaa gatgtgggac
     5041 gtgaccgcgg ttggcgcacc cgcctaggtt cgtagcgcac cagtccatcg aatccacgga
     5101 gttgaagtga acccgaagaa gttgctcgcc accctgggcg cgggagtgtt ggcgctgtgc
     5161 gccgtggccc cgtcggctac cgcagagccg gccgcggggt cgaaggccaa tggctcgtcc
     5221 acggctccgg atgtcaccac caacggccgg cacgtcgtct tctcctccgt cgccaccgac
     5281 ctggtcaccg gcgacaccaa cggcaggtcc gacatcttcc tgcgggacat cgccttcaag
     5341 aagaccaaga tcgtcaccaa gagctacacc gggggaccgg ccaacggcaa cagcaccgag
     5401 ccgcaggtca gcgacgacgg ccgctatgtc gtctacatgt ccgacgcgac gaacctggtg
     5461 cccggggaca ccaacggcaa gacggacatc ttccgcaccg acatgaccac gatgaagacg
     5521 acccggatca gcacgtcgca aagcggcgcc caggcgaacg gcgtctccaa gcggccccgc
     5581 atcaacagcg ctgccacggt catcgcgtac gtgagcacgg caccgaatct cgtgccgggc
     5641 gacaccaacc ggattcagga catcttcgtc cgcaacctga acaacaacag cgtcaagcgg
     5701 atctcggtca gcccgacggg cgcgcaactg agcgtcatgc ccttctcgcc gtcggtgtcc
     5761 tatgacggca agatcgtggg cttccacacc gacgcctacg ccaaccccac ggtcaactcc
     5821 tactgcggcc gcaccgccga cgccctctcg gtcatcatga agtggaccgt ctcgacgtcc
     5881 aagtcgagca tcacggaggt cgtctgccgc cagagcgggg tgcgcaggca ggtctacgag
     5941 ccgcgcgcca acatgcagaa caaggtcggc tacgccgtcg cctccgactg gggctacacg
     6001 gaattcttcg cccgcgggct ctacaccctc tacgactcga tcgtcgaggg ctcgtcctac
     6061 tcgactccct actgggatgt ggcgcgttgg ggcggcac
//
LOCUS       DAOPJG010000192         6067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_13978_length_6067_cov_6.904691, whole genome shotgun sequence.
ACCESSION   DAOPJG010000192 DAOPJG010000000
VERSION     DAOPJG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13978_length_6067_cov_6.904691"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..240)
                     /locus_tag="PKU75_14155"
     CDS             complement(109..240)
                     /locus_tag="PKU75_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82068.1"
                     /translation="MRAFLGIFGPGLVTGVADDDPSGVATYSQAGAAAGSACSGQHR"
     gene            complement(493..>1377)
                     /locus_tag="PKU75_14160"
     CDS             complement(493..>1377)
                     /locus_tag="PKU75_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF82069.1"
                     /translation="LPAYDCFEGHQSLVELDHDAEEVVDLVADVMTHWLERGIDGWRL
                     DAAYAVPQHFWARVLPRVRERFPECYILGEVIHGDYPAFVAESGVDAVTQYELWKAIW
                     SSLASANFWELSHALGRHATFLESFVPQTFVGNHDVTRIATQIEDPRHLGHAYAVLLT
                     VGGTPSIYYGDEMGYTGTKEQRVGGDDQVRPVMPATPAELGLGHDTYAMVKSLVAVRR
                     RHPWLHTARATDLTLTNEVYAYDLRGPDGERIVVCLNISAEPATFDDVPATVLAGDAA
                     LATSSVMVAPHSWAVLGD"
     assembly_gap    1378..1477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1680..2378
                     /locus_tag="PKU75_14165"
     CDS             1680..2378
                     /locus_tag="PKU75_14165"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPF82070.1"
                     /translation="MPRAAYALKLAYGALAVADTALAGSRSTRAHRIRRVTKPLLMPV
                     LTGAFAADPRSARSPLRDTTRAGQALGWLGDVALLRPGLPAFAAGVGAFGLGQAAYIR
                     GFRANASPVPLRHTTAGRAAAVMFALSGAPMAIAAARQERVLGPAVLGYAALLTAMAA
                     NAANLDPALPAGARAATAAGGAAFLVSDTILGASQFLLADPPPWVESAVMASYTAAQF
                     LLAEGALRCGGSGS"
     gene            complement(2514..4808)
                     /locus_tag="PKU75_14170"
     CDS             complement(2514..4808)
                     /locus_tag="PKU75_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82071.1"
                     /translation="MPHDASVQQIQTLTRRTFLGGGAAALAAYAAAPAPAAALDLGAG
                     SGYWLGERYWGNRLQDWLSRAGRMECVAEPGMGVGRTVALLTHEIRTGDFDLSVRTGT
                     LALGEGLSGFVLGTGVPGEDARRRALLGSASGVGGGLIAYVDHNGAVGFRDHTPEGAQ
                     LNWPAMSATASGTGSPRSLDEDLTLAVTCRTVDETTVSLRVAVRRTSDDAELTSATIA
                     ALPVTSVRGGIALMSSSRQGSESRYWFTGLSATGSGLTVIPERTLGPVVGTLFTSVAG
                     VLRMTVQCMPVDPRDLPSVSLEVLRSGSWIRLGTNQIGPGYTAQFYASGMDPTTPTRY
                     RIVAGDQTLYGGTIPAEPRGGIRIASLNCLKASHRPLDAPSAWQARLPGSVSLDLYSR
                     RNIYFPHNEIVDSITAQRPDLLVAHGDQLYETSPTRKDTSSAPELDFLYKFLLWHWAM
                     RPLTRRLPTVVLTDDHDVYQPNLYGEGGIATTDFRTGGYVKAASWVNLVQRMSSWHNP
                     DRFDATPVAQGITVGYAAFSYGGTSFAIVEDRKFKTGLIKPGATPQLLGARQEAFLGT
                     WANMHPGQPKVILTGSTWACVQTDADGAPRLDADSNGWPAPGRMRAVKLARAAHALIV
                     SGDQHVGHLVRHGIAGFADGPLQFTPPAGSTSFQRWFQPAQPLPNSAGTPDTGDFTDR
                     FGNRLRVLAAVNPKFTLRQLWDHYPAPGQDFGDRNLKREGFGLIAFDHARRRVRIECW
                     PWNATETGAGQYPGWPVTVPYSAL"
     gene            4973..>6067
                     /gene="eno"
                     /locus_tag="PKU75_14175"
     CDS             4973..>6067
                     /gene="eno"
                     /locus_tag="PKU75_14175"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPF82072.1"
                     /translation="MATIEAIVAREILDSRGNPTVEVEVGLDDGTVARAAVPSGASTG
                     AFEAVERRDGDKKRYLGKGVEQAVDAVMEEIAPKLYGYDAADQRLVDNEMLSLDGTPN
                     KGELGANAILGVSLAVAKAAAESAGLPLFRYVGGPNAHVLPVPMMNILNGGSHADSNV
                     DIQEFMIAPIGAGSFREALRWGAEVYHALKGVLHDKGLATGLGDEGGFAPNLESNAAA
                     LDLILEAIKKAGYKPGTDIALALDVAASEFYDKGSYTFEGTKKSSDEMTAYYVDLVAK
                     YPLVSIEDPLNEDDWDGWAKITEALGDKVQLVGDDLFVTNPERLAKGISTKTANALLV
                     KVNQIGSLTETLEAVDMAHRAGYTCMMSHRS"
BASE COUNT          941 a         2098 c         2047 g          881 t
ORIGIN      
        1 cgcgtgcgct gcggacgaat ttgcgccgca cgagggtgcc gaggctgtcg ccggtgacca
       61 atgcggtgcg atcgcagatc tcctgcacgg catacagcaa cggcaaggtc agcggtgctg
      121 tccagagcag gccgacccgg cggcggcgcc ggcctgggag taggtggcga ctccggaggg
      181 atcgtcgtcc gccacgccgg tcaccagccc tggcccgaag atgccgagga aggcgcgcat
      241 ccgtgggcgg cgatcagccg tggacggcgg cggcggggcg tccaggtctg ggaccggcga
      301 ggtcgtctcg gacgtcctcg ctctccggcg gaacaggctg cggtgccagg ggtgttgccc
      361 cactgaactg ctcacgtgca gatgacatgg cgatccttac gcactccggg ccgggaaccc
      421 atggcgcatc gtcgtcgccg acgctgagat gcggctgaga gatcgccgca aatcacctga
      481 gcagccggag aatcagtccc cgaggacggc ccaggagtgc ggggccacca tgacgctcga
      541 ggtagcgagg gcggcgtcgc ccgccagcac ggtggccggc acgtcatcga aggttgccgg
      601 ttcggcggag atgttgaggc acacgacgat tcgctcgccg tctgggccgc gcaggtcgta
      661 ggcgtagacc tcgttggtca acgtcaggtc ggttgcccgc gccgtatgca gccacgggtg
      721 gcgccggcgc acggcgacga gggacttcac catcgcgtac gtgtcgtggc ccagtccgag
      781 ttcggcaggg gtggcgggca tgacggggcg cacctggtca tcgccgccga cgcgttgctc
      841 cttggtgccg gtgtagccca tctcgtcgcc gtagtagatc gacggcgtgc cgccgacggt
      901 gaggagcacg gcatacgcgt gcccgaggtg gcgcgggtcc tcgatctggg tggcgatgcg
      961 cgtgacatcg tgattgccga caaaggtctg gggcacaaag gattccaaga aggtcgcgtg
     1021 ccgtccgaga gcgtgcgaca actcccagaa gttggcgctc gccaggctcg accagatggc
     1081 cttccacagt tcgtactgtg tgaccgcgtc gaccccggac tcggcgacga aggccggata
     1141 gtcgccgtgg atgacttcgc cgaggatgta acactccggg aaccgctcgc gaacgcgcgg
     1201 cagcacccgc gcccagaagt gttgcggcac agcgtaggcc gcgtccagac gccacccgtc
     1261 gatcccgcgc tcgagccagt gcgtcatcac gtcggcgacg aggtcgacga cctcctcggc
     1321 gtcgtggtcg agctcgacga gcgactggtg accctcgaaa cagtcgtagg ccggcaannn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc agccacgcct cgatgtggcc
     1501 gagacggttc tccggctcca cctccgcgct gccgggcacg tgggcggccc cgacgaaccc
     1561 caagggatag atgtgccacc acaacacgtg ctcgacccag ccggtcatcc gcactccttc
     1621 acgttcgatc atcgccttca ccctgtcata cggcgggctc ccggatacca tccggcggta
     1681 tgccgcgcgc cgcatacgct ctcaagctcg cgtacggcgc gctcgccgtc gccgacacgg
     1741 ccctggccgg ttcgcgctcc acgcgcgccc accgcatacg acgcgtcacc aaacccctgc
     1801 tcatgccggt gctcaccgga gccttcgccg ccgacccgcg atcggcgcgc tcgccgctgc
     1861 gagacacgac ccgcgccggg caggcactgg gctggcttgg ggacgtcgcc ctgttgcgcc
     1921 ccggcctgcc ggccttcgcc gccggggtgg gcgctttcgg gctcgggcag gccgcctata
     1981 tccgcggctt ccgggccaac gcgagcccag ttccgttgcg ccacaccaca gccggtcgcg
     2041 ccgccgcagt gatgttcgca ctctccggcg cgccgatggc catcgcggcc gctcgccaag
     2101 agcgcgtcct cggccccgcc gtcctggggt atgccgcgct cctgacggcc atggccgcca
     2161 acgccgcgaa cctcgacccc gctctcccgg ccggtgcccg cgccgcgacg gccgccggcg
     2221 gtgcggcctt cctggtctct gacaccatcc tcggcgcgag ccagttcctc ctcgccgacc
     2281 cgccgccctg ggtggaatcg gcggtcatgg ccagctacac cgcggctcag ttcctcctcg
     2341 ccgagggcgc gctccgctgc ggcggatctg gcagctgaga gaggattcac tcctgcgagc
     2401 cgccggccca acctggagca ggagcacacc ccacaggccg gtccaccatg ccatcgcggg
     2461 ttcgcccgga tcagcagctc gatcgtgggc tcgcccgaac cgtcgatctc gattcagagc
     2521 gcggaatagg gcaccgtgac cggccagccc gggtactggc cggctccggt ttcggtggcg
     2581 ttccacggcc agcactcgat ccgcaccctg cggcgggcgt ggtcgaaggc gatgaggccg
     2641 aagccctcac gcttgaggtt gcggtcaccg aagtcctgcc cgggcgccgg gtagtggtcc
     2701 cacaactgac gcagcgtgaa cttcgggttc acggccgcca gcactcggag ccggttgccg
     2761 aagcggtccg tgaagtcgcc ggtatccggc gttcccgccg agttgggcag tggctgtgcc
     2821 ggctggaacc agcgttggaa gctcgtcgac ccggcggggg gagtgaattg cagcggcccg
     2881 tcggcgaatc cggcgatgcc gtgccgcacc aagtggccca catgctgatc gccggacacg
     2941 atgagcgcgt gcgctgcacg tgccagcttc acggcccgca tccggccggg ggccggccag
     3001 ccgttgctgt cggcgtccag gcgcggggcc ccgtccgcgt cggtctgcac gcacgcccag
     3061 gtcgatccgg tgaggatcac cttcggctga ccgggatgca tattcgccca cgtgccgagg
     3121 aaggcttcct gccgcgcccc gagcaattgc ggtgtcgcgc ccggcttgat gagtccggtc
     3181 ttgaacttgc ggtcctcgac gatggcgaac gacgtgccgc cgtagctgaa cgcggcatac
     3241 ccgacggtga tgccttgggc tacgggcgtc gcgtcgaaac ggtcggggtt gtgccacgag
     3301 gacatgcgct gcaccaggtt gacccagctt gccgccttga catatccgcc ggtccggaag
     3361 tccgtggtgg cgatgccgcc ttcgccgtag aggttcggct ggtagacgtc gtgatcgtcg
     3421 gtgaggacca cggtcggcag gcgccgggtc agcgggcgca tcgcccagtg ccacagcagg
     3481 aacttgtaga ggaagtccag ttccggtgcc gagctggtgt ccttgcgtgt gggtgacgtc
     3541 tcgtagagct ggtcaccgtg ggcgaccagc agatccggcc gctgggcggt gatgctgtcg
     3601 acgatctcgt tgtgcgggaa gtagatattg cgccggctgt agaggtcaag ggacaccgag
     3661 ccaggcaggc gggcctgcca ggcgctcggc gcatcgagcg gacggtggct cgccttgagg
     3721 cagttcaggc tcgcgatccg tatgccgccg cgcggctcgg ccggaatggt gccgccgtag
     3781 agggtctggt ctccggcgac gatgcggtag cgggtcggcg tggtggggtc catgccggag
     3841 gcatagaact gcgccgtgta gcccggtccg atctggttgg tgcccaagcg aatccagctg
     3901 ccggagcgaa gcacctccag gctcaccgac ggcaggtcac gcgggtcgac cggcatgcat
     3961 tgcacggtca tccgcaagac gcccgccacc gacgtgaaga gggtgcccac caccggaccc
     4021 agcgttcgct ccgggatcac ggtcagcccg gagccggtcg cggacaatcc ggtgaaccag
     4081 tagcgggact ccgacccctg ccggctggag gacatcagcg cgatcccccc gcggacgctc
     4141 gtcacgggga gggcggcgat ggtggcgctg gtcagttcgg cgtcatccga cgtccggcgc
     4201 acggcgaccc gcaaggagac ggttgtctcg tcgaccgtgc gacaggtcac ggccagggtc
     4261 agatcctcat cgaggctccg ggggctgccc gtgccgctcg ccgtggcgga catcgccggc
     4321 cagttgagct gggccccctc cggggtgtgg tcgcggaaac cgaccgcacc attgtgatcg
     4381 acataggcga tcagtccacc ccccaccccg ctcgccgacc cgagcagcgc ccgccgccga
     4441 gcgtcctcgc ccggcacgcc cgtgccgagg acgaaaccgg agaggccctc gccgagagcc
     4501 agggtgccgg tgcgcaccga gaggtcgaag tcgccggtcc ggatctcgtg cgtcagcaac
     4561 gccacggtgc gccccacccc catccccggc tccgcgacgc actccatccg cccggcacgc
     4621 gacagccagt cctgcaggcg gttgccccag tagcgctcgc cgagccaata acccgacccc
     4681 gcgccgaggt ccaacgcggc agcgggggcc ggcgcggcgg catacgcagc gagcgcggca
     4741 gcgcctccgc cgaggaaagt ccgccgggtc aaagtctgaa tctgctggac agaggcatcg
     4801 tggggcatgg ccgggagtct ggcacggggc cgacgcggat gtcgcgtacc aggtagtcgg
     4861 cgcctcatca ccgggagttg gtgggtcggt cacgaatagg caacggagac agggctaggc
     4921 tgggacccga gccaatctgg gccctcatct gccatcacca aggagtgatt ccgtggccac
     4981 catcgaggca atcgtcgcgc gcgagatcct ggactctcgc ggcaacccca ctgtcgaggt
     5041 cgaggtcggc ctcgacgacg gcacggtcgc gcgggcggcc gtccccagcg gcgcctccac
     5101 cggcgccttc gaggccgtcg agcgccggga cggcgacaag aagcgctacc tcggcaaggg
     5161 cgtcgagcag gcggtcgacg cggtcatgga agagattgcc cccaagctct acggctatga
     5221 cgcggccgac cagcggctcg tcgacaacga gatgctgtcc ctggacggca cccccaacaa
     5281 gggcgaactc ggcgccaacg ccatcctcgg cgtctcgctc gccgtcgcca aggcggccgc
     5341 cgagtccgcc gggctgccgc tgttccgcta tgtcggtggc cccaacgccc acgtgctgcc
     5401 cgtcccgatg atgaacatcc tcaacggcgg cagccacgcc gactccaatg tcgacatcca
     5461 ggagttcatg atcgccccca tcggcgccgg ctccttccgc gaggcgctgc gctggggtgc
     5521 ggaggtctac cacgccctca agggcgtcct gcacgacaag ggcctggcca ccggcctcgg
     5581 cgacgagggc ggcttcgccc ccaacctcga gtccaacgcc gccgcgctcg acctgatcct
     5641 cgaggcgatc aagaaggccg ggtacaagcc gggcacggac atcgcgctcg cgctcgacgt
     5701 cgccgccagc gagttctacg acaagggctc ctacaccttc gaaggtacca agaagtccag
     5761 cgacgagatg accgcttact acgtcgacct ggtcgccaaa tacccgctgg tgtccatcga
     5821 ggacccgctc aacgaggacg actgggacgg ctgggccaag atcaccgagg ccctcggcga
     5881 caaggtgcag ttggtcggtg acgacctgtt cgtcaccaac cccgagcggc tcgccaaggg
     5941 catctcgacc aagaccgcca acgcgctgct ggtcaaggtc aaccagatcg gctccctgac
     6001 cgagaccctc gaagcggtcg acatggccca ccgcgccggc tacacctgca tgatgagcca
     6061 ccgctcc
//
LOCUS       DAOPJG010000193         6017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14133_length_6017_cov_7.807615, whole genome shotgun sequence.
ACCESSION   DAOPJG010000193 DAOPJG010000000
VERSION     DAOPJG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14133_length_6017_cov_7.807615"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1265
                     /locus_tag="PKU75_14180"
     CDS             168..1265
                     /locus_tag="PKU75_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPF82073.1"
                     /translation="MRSVAALEASQRVRFCRSADGTRIAYAVHGTGPPLLLDACWLSH
                     LEYDWQSPVWRHYLVELGRVATVVRFDERGHGMSDRDVADFSLERRIEDLEAVTDHAG
                     LDRFALMAMAQGGPVALHYAHRHPERVTRLVCASTYAASSQHVTDDDRALEEAFQAMI
                     RAGWDRKDPLFRRVFTSMMIPDASEEQMVWLDELHHRSASAKTAYESRVERGRADATG
                     VLPDLTVPTLVIHSRHERMNDFEHGRSLAAGITDARLVPLDSRNHILLEDEPAWPVFV
                     REVTAFLAGDASASGDAGLGVGSLSTRELEVLRLVAQGRDNSSIAADLTLSVRTVERH
                     LQRIYAKLGLSGPSARAGAVAILLRATPTER"
     gene            1389..1751
                     /locus_tag="PKU75_14185"
     CDS             1389..1751
                     /locus_tag="PKU75_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPF82074.1"
                     /translation="MNKAAISVTTGLEDPEKVTVALLVAVGAAESGRPTLMFLTKEAV
                     RLAVPGHARAVACDGCPPLDNLMQRYAAAGGTMIACGICLDAKGIDGDDLVDNATKAG
                     SVQLWEWIGDDPATTFSF"
     gene            1770..2363
                     /locus_tag="PKU75_14190"
     CDS             1770..2363
                     /locus_tag="PKU75_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPF82075.1"
                     /translation="MFISTTPTPEAETAFAAYVDSQRDAWGFVPDYTGCFAARPEVAT
                     AWIALASAVRTTMDRRRFELVTIAAARARGSAYCTAAHAKMLRDVCRDEPTLRGLVKD
                     PDGGQLGAVDAAIYRFATQVATAPSSVQQPDVDKLRTLGLGDADIAHIAYAVGVRLFF
                     ATVLDALGAQPDPETLAVLDQELLTFVGASPDIEQGP"
     gene            complement(2549..2926)
                     /locus_tag="PKU75_14195"
     CDS             complement(2549..2926)
                     /locus_tag="PKU75_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82076.1"
                     /translation="MDILTEVEALADRVSIIRLGEIVQTGTLKDLRAQTHVSISAVLE
                     RRPDAAGALEVLDEVRLDEHNRLTAAVAPDQVNAAMAALVPYGMSRLTVSPASLEDLF
                     LREYAEGQGAAETAKSVAAEGNR"
     gene            3095..3556
                     /locus_tag="PKU75_14200"
     CDS             3095..3556
                     /locus_tag="PKU75_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPF82077.1"
                     /translation="MKPLGGEVTLHIDAPPGRVWALVTDVTRIGEFSPETVAGRWTGG
                     STGPEVGATFAGKVIRNGVGPTYWSACQVTRCEPERLFEFSVGTKKVAVNNWGYRLEP
                     SGAGTDVTEYYRLEPMLPVRLYWALLGRGRGRTNENGMRTTLERMKSVLEG"
     gene            complement(3571..4110)
                     /locus_tag="PKU75_14205"
     CDS             complement(3571..4110)
                     /locus_tag="PKU75_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82078.1"
                     /translation="MAEHGSAPTQTGGGLAAGEREQLLYVLESRFAPALEAATAAVRD
                     AERVLAEARSRLARAESAANAPYASDPLIFMRRSVEDEVDGLERKTTERKVRASYRFL
                     LDRSVELAMAEVARFHDDQATLHRELAEGVDACRAAEGRAAEALVEAQRMQERVRASE
                     QTARRGLHLLQEKLGPNRS"
     gene            complement(4154..4471)
                     /locus_tag="PKU75_14210"
     CDS             complement(4154..4471)
                     /locus_tag="PKU75_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82079.1"
                     /translation="MAGAEVYGALWCELATHAMRQEPYAAGLADVMVAAQLRESTRIY
                     ARLTTPAKAARLARLTVAVGQDLIFDLLIDRDRRAADAAVREFSRMVRRELDGGVIRH
                     ECR"
     gene            4475..4678
                     /locus_tag="PKU75_14215"
     CDS             4475..4678
                     /locus_tag="PKU75_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82080.1"
                     /translation="MPPVAFPGQDRVGLVDEGPADLFGLPPGDGDGQVDARATWIALA
                     WCAGITLVCGWWATRTYAAKPAR"
     gene            4787..5317
                     /locus_tag="PKU75_14220"
     CDS             4787..5317
                     /locus_tag="PKU75_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPF82081.1"
                     /translation="MSIERPVAPDPYDLLPATASFSVTSTDVTDGQPLKDDQVAHLGN
                     TSPQLSWEGAPEGTGSYVVTCFDPDAPTPCGFWHWAVVDLPASVTSLDTGAGADGGAA
                     LPAGAFMLRNDGGSASFMGAAPPEGDQVHRYYFVVHAVGPESLGIDADASNAVMSFNL
                     AFKTLGRAIVKGTYQH"
     gene            complement(5468..5776)
                     /locus_tag="PKU75_14225"
     CDS             complement(5468..5776)
                     /locus_tag="PKU75_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82082.1"
                     /translation="MWTQDGGPWRAGPMSLYLKSGFWQLYDLNVYAGGRLGWDPSSDL
                     GAVNADWIAHTFGDDPAVVRAVGEVLNRSRETVLDGLYIRPYAVRQVFARGSGPSGIP
                     "
     gene            complement(5722..>6017)
                     /locus_tag="PKU75_14230"
     CDS             complement(5722..>6017)
                     /locus_tag="PKU75_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82083.1"
                     /translation="LFADFHPKNLVVSTKFTMGDFDSWLPLNPTLGVGEQARLVEMQG
                     RREFEAFNAIPDDMGSIIRSPCSVSSPATPTSTGCGCGPRTAGRGGPDRCRSI"
BASE COUNT          916 a         2155 c         2022 g          924 t
ORIGIN      
        1 atccggctgg gtttccgctc gcgcgcccaa gtggccgcct ggttcgtcac gcaggagccc
       61 tcatcccctc aagtgcggta gatctgcgcc tgactgagtc gcgccgggca cgcgcgagct
      121 gttgtgccca acacgacggg ttgcccgcat ctccctacac tgtctagatg cgcagcgtcg
      181 ctgcacttga ggcgtcccag cgggtgcggt tctgccggtc cgcggacggg acgcggatcg
      241 cgtatgccgt gcacggcacc ggtccaccgc tcctgctcga cgcctgctgg ctcagccacc
      301 tggagtacga ctggcagagc ccggtctggc gccactacct cgtcgagctg ggccgggtcg
      361 ccaccgtcgt gcggttcgac gagcgcgggc acggcatgtc ggatcgtgac gtggccgact
      421 tcagcctgga gcggcgaatc gaggacctcg aggccgtcac cgaccacgcc ggcctggacc
      481 ggttcgcgct catggcgatg gcccagggcg ggccggtcgc gctgcactac gcacatcgtc
      541 accccgaacg ggtgacgagg ctggtctgcg cgagcacata tgccgcgtcg tcgcagcacg
      601 tgacggatga cgaccgggcc ctggaagagg ccttccaagc gatgatccgt gccggctggg
      661 accgcaagga tcccctcttc cggcgcgtgt tcacctcaat gatgatcccc gacgcatcgg
      721 aggagcagat ggtgtggctc gacgagctgc accatcgctc cgcgtccgcg aagacggcat
      781 atgagtcccg ggttgagcgg ggccgggcgg acgccaccgg tgtccttccc gacctcaccg
      841 tgccgaccct cgtcatccac tcgcggcatg agcggatgaa cgacttcgag cacggacgct
      901 ccctcgccgc cggcatcacc gacgcccggc tcgtgcccct cgacagccgc aaccacatcc
      961 tcctcgagga cgagccggcc tggccggtct tcgtccgtga ggtgacggcc ttcctggccg
     1021 gggacgcctc ggcgtcaggt gatgccggcc tgggtgtggg ctccctgtcc acgcgcgagc
     1081 tggaggttct gcgcctggtc gcgcaaggcc gtgacaactc ctcgatcgcc gcggacctga
     1141 ccctgagcgt ccgcacggtc gagcggcacc tccagcggat ctacgcgaag ctgggactgt
     1201 cgggcccgtc cgctcgcgcg ggtgcggtgg cgatcctgtt gcgcgccaca cctactgagc
     1261 gctgaccgac agccggctcc cgggacgacg cggcaggttg agtggtggcg cgctgttgca
     1321 gccgtcagcc gtcggcctag cgtcgtgatc actggccaag gggtcggtga cacggacagg
     1381 agcgcagcat gaacaaggca gccatcagtg tgacgaccgg cctcgaggat ccggagaagg
     1441 tcaccgtggc cctgctcgtc gccgtcggtg ccgccgagtc gggtcggccg accttgatgt
     1501 tcctcaccaa ggaggcggtg cgcctcgcgg tgcccggtca cgcccgcgcc gtcgcgtgcg
     1561 acgggtgccc tccgctggac aacctcatgc agcggtatgc cgcggccgga ggaaccatga
     1621 tcgcctgcgg aatctgcttg gacgccaagg gaatcgacgg cgacgacctg gtcgacaacg
     1681 ccaccaaggc cggctcggtc cagctgtggg aatggatcgg cgacgacccg gcaaccacct
     1741 tcagcttctg acctccgaca gcgactgccg tgttcatctc gacaaccccg accccggagg
     1801 ccgagaccgc cttcgcggcc tacgtcgaca gccaacgcga tgcctggggc ttcgtccccg
     1861 actacaccgg ctgcttcgcc gcccggcccg aggtcgccac ggcgtggatc gccctcgcct
     1921 ccgcggtacg aacgaccatg gaccggcgac gcttcgaact ggtcaccatc gctgccgccc
     1981 gcgcgcgcgg ctcggcctac tgcaccgccg cgcacgcgaa gatgctcagg gacgtctgcc
     2041 gcgacgagcc cacgctccga gggctcgtca aggacccgga cggcggccag cttggcgccg
     2101 tggacgccgc catctaccgg ttcgcgaccc aggtcgcgac tgcccccagc tcagtgcagc
     2161 agcccgacgt ggacaagctt cgcaccctcg gcctgggcga cgcggacatc gcgcacatcg
     2221 cgtatgccgt gggcgtccgc ctgttcttcg cgaccgtgct cgacgcactc ggcgcccaac
     2281 ccgatcccga gaccctggcc gtgctcgacc aggaacttct taccttcgtt ggagcttcac
     2341 cagacatcga acaaggcccc tgacctaccc gttcggccac ttccactgag gagtgtggga
     2401 cgactgcgac ggattatggg actggcgccc tgggctacat cccaatctgg cggttgatcg
     2461 cggtcgctcc gatctcgact gggcgcgata ttgcgggcgt cctgccggat ggacgccgcg
     2521 gtcagggtgc gcgtgccggt gaccgcgctc accggttgcc ctctgcagca acggacttgg
     2581 cagtctcggc agcgccctgg ccctcggcgt attcgcgcag gaagaggtct tcgagggagg
     2641 ccggagagac ggtcagccgg gacatcccgt agggcaccag cgccgccatc gcggcgttga
     2701 cctgatcggg agcaaccgcg gcggtgagcc ggttgtgctc gtcgaggcgc acctcgtcca
     2761 gcacctcaag agcgccggcc gcgtcgggac ggcgctcgag gacggccgag atcgacacgt
     2821 gggtctgagc gcgcaagtcc ttcagcgtgc cggtctggac gatctcgccg aggcggatga
     2881 tgctgacccg gtccgccagg gcctccacct cggtgaggat gtccatttgg tgtgcgtatg
     2941 ccggggccga agcaccagaa cgtcgacccg acgcgcgccg cctcccgacc catcagacat
     3001 cagcgaaggg catcgaaggt cgttgcgcga cggagcgtct acgctggccg tgagccggcc
     3061 gggccccggt cgggcacacg agacggaggc gcggatgaaa ccgctcggcg gggaggtcac
     3121 cctgcacatc gacgctccac cagggcgcgt gtgggcgctg gttaccgacg tgacccggat
     3181 cggggagttc agccccgaga ccgtggccgg ccgctggacc ggtggctcga ccggtcccga
     3241 ggtcggcgcc acgttcgccg ggaaggtgat acgcaatggt gtcggcccga cctattggtc
     3301 ggcgtgccag gtgacccggt gtgagccgga gcggctcttc gagttctccg tcggtaccaa
     3361 gaaggtcgcg gtcaacaatt ggggataccg gttggaaccg tcaggagccg ggaccgacgt
     3421 caccgagtat taccggctcg agccgatgct gccggtccgt ctctactggg cgttgctcgg
     3481 ccgcggccgc ggccgcacca acgagaacgg gatgcggacc acgctggagc ggatgaagtc
     3541 ggtcctcgaa gggtgacccg ggcccgccgg tcaggagcgg ttggggccga gcttctcctg
     3601 cagcaggtgc agcccgcggc gggcggtctg ttcgctcgca cggacgcgtt cctgcatccg
     3661 ctgggcctcc accagagcct cggcggcgcg gccctccgcc gcccggcagg catccacacc
     3721 ctctgcgagc tcccggtgca gcgtggcctg gtcatcgtgg aagcgcgcca cctcggccat
     3781 cgccagttcc accgaccggt cgagaaggaa acggtatgac gccctgacct tcctctccgt
     3841 cgtcttgcgc tccagcccat cgacctcgtc ctcgacggaa cggcgcatga agatcagcgg
     3901 gtccgacgcg tacggcgcgt tcgctgccga ttcggcgcgg gccagacggc tcctggcctc
     3961 ggccagcacg cgctcggcgt cccggaccgc ggcagtggcc gcctccaggg ccggcgcgaa
     4021 ccgggactcc aggacataca gcaactgctc gcgctccccg gcggcgagac ccccaccggt
     4081 ttgcgtcgga gcgctaccgt gctcagccac gtggacagcc ttcgcgagtc gagttcatta
     4141 cgacaccctc tcatcaccgg cactcgtggc gaatcacgcc accgtccagc tcgcgccgca
     4201 ccatccggct gaactcccgc accgccgcgt ccgcggcccg ccggtcacgg tcgatgagca
     4261 ggtcgaagat gagatcctgc cccaccgcga cggtcagccg ggccagccgg gccgcctttg
     4321 ccggcgtggt aagcctcgcg tagatccggg tggactcgcg cagttgcgcg gccaccatga
     4381 cgtcggcgag cccggcggca tacggctcct ggcgcatggc gtgggtcgcg agctcacacc
     4441 acagcgcgcc gtagacctcg gcacccgcca cggtatgccg ccagttgcgt tccccggcca
     4501 ggaccgggtc gggctcgtcg acgaagggcc ggccgatctg ttcggcctgc cccccggcga
     4561 tggcgacggg caggtcgatg cccgggcgac gtggatcgcg ctggcgtggt gcgcaggtat
     4621 cactctcgtc tgcgggtggt gggcgacccg gacgtatgcc gccaagcccg ctcggtgaga
     4681 gccgaccaac tcatctgagc ggtagcgacc gccccgtgtc gctcccctca gatgacggac
     4741 gggcgcccgc tgccggatcg gcccgcgcct ggctatcgtg atttgcatga gcatcgaacg
     4801 tcccgtcgcc ccggatccct acgacctcct cccggccacc gcgtccttca gcgtcaccag
     4861 taccgacgtg accgatgggc agcccctcaa ggacgaccag gtcgcccacc tcggcaacac
     4921 ctccccgcag ctgtcgtggg agggggcgcc agagggcact gggtcgtatg tcgtgacctg
     4981 cttcgacccg gacgcgccga ccccatgtgg gttctggcac tgggccgtcg tcgaccttcc
     5041 cgcgtccgtc acctcgctcg acacgggagc cggcgccgac ggcggggccg cccttcctgc
     5101 gggcgccttc atgctgcgca acgacggcgg gtcggcgtcc ttcatgggcg cggctccacc
     5161 cgagggcgat caggtgcacc gctactactt cgtcgtgcac gccgtcggcc cggagagcct
     5221 gggcatcgac gccgacgcct ccaacgccgt catgagcttc aacctggcct tcaagaccct
     5281 gggccgagcc atcgtcaagg gcacctatca gcactgacac acgtcgtcag gacgagccac
     5341 gcacccaaca aggttcggct ggacgacccc gccgccgggt cccgacgtcg cgtcaccggc
     5401 ttgcagggtg gtgaacaggt caacctcgta gtcgacggat cgcaccagct actcccccag
     5461 cgccgggtca cggtatgccg ctgggcccac tcccgcgagc gaagacctgc cgcacggcat
     5521 acgggcggat gtagagccca tcgaggacgg tctcccgcga gcggttcagc acctcgccca
     5581 cagcgcgcac cacggcgggg tcgtctccga aggtgtgcgc gatccagtcg gcgttcaccg
     5641 cgccgaggtc gctcgacggg tcccagccga gacggccacc ggcatacaca ttgaggtcgt
     5701 acagctgcca gaacccgctc ttcagataga gcgacatcgg tccggcccgc cacggcccgc
     5761 cgtcctgggt ccacatccac agcccgtcga tgtgggggtt gccggcgagg aaacgctgca
     5821 gggcgacctg atgatggagc ccatgtcgtc ggggatcgcg ttgaaggcct cgaactcgcg
     5881 gcggccctgc atctcgacca ggcgggcctg ctccccgacg ccgagcgtcg gattgagcgg
     5941 cagccacgag tcgaagtcgc ccatcgtgaa tttcgttgag acgacaaggt ttttggggtg
     6001 gaagtcggcg aagagcc
//
LOCUS       DAOPJG010000194         6005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14175_length_6005_cov_7.843866, whole genome shotgun sequence.
ACCESSION   DAOPJG010000194 DAOPJG010000000
VERSION     DAOPJG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14175_length_6005_cov_7.843866"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PKU75_14235"
     CDS             complement(<1..802)
                     /locus_tag="PKU75_14235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493254.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPF82084.1"
                     /translation="MTATVTDSAQDTQQGGVGRISRIIGPVVDVEFPADAMPDQYNLL
                     TTEVSLAGEGGGEGTKTLNLEVAQHIGDNMVRAISLQPTDGLVRGTAVQDSGGPITVP
                     VGDVTLGHVFNTTGVCLNLEEGETLDVQERWGIHRPAPAFDQLESKTVMFETGIKVID
                     LLTPYVQGGKIGLFGGAGVGKTVLIQEMIARVARDHGGVSVFAGVGERTREGNDLMVE
                     MEEAGVLGQTALVFGQMDEPPGTRLRVALSALTMAEYFRDVQKQDVLLF"
     gene            complement(814..1722)
                     /locus_tag="PKU75_14240"
     CDS             complement(814..1722)
                     /locus_tag="PKU75_14240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HPF82085.1"
                     /translation="MGAQIRVYRQRIRSVSATKKITRAMELMAASRVVKARTAVLESA
                     PYTHALTTALSALATHSNYDHPMTTEVENPTRAGVLILTSDRGLAGAYASGTMKESER
                     LIGELRADGKEVVPYLVGRKAVSYYKFRGRDYAAEWTGNTEKPQVERAREISERLIED
                     FLRPTEEGGLDEIHVVYTRFNTMVNQEARVLRLLPIEVVDAPEDANHDGVKDILPLYQ
                     FEPNAEEVLHTMLPRYVTARIWTCLLSAATSELAARQRAMKSATDNAENLINTYTRLA
                     NQARQAEITQEISEIVGGASALAESK"
     gene            complement(1767..3401)
                     /gene="atpA"
                     /locus_tag="PKU75_14245"
     CDS             complement(1767..3401)
                     /gene="atpA"
                     /locus_tag="PKU75_14245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPF82086.1"
                     /translation="MTELSIRPEEIRDALDAYVQSYQPGQASREEVGRVIDAGDGIAH
                     VEGLPSAMTNELLQFEDGTLGIALNLDVHEIGVVILGDFSKIEEGQTVKRTGEVLSVP
                     VGDAFLGRVVNPLGQPIDGLGDIADEGRRALELQAPNVVSRKSVHEPLQTGLKAVDAM
                     TPIGRGQRQLIIGDRQTGKTTVAVDTIINQKEFWDTGDPTKQVRCIYVAIGQKGSTIA
                     SVRGTLEDAGALEYTTIVAAPASDSAGFKYLAPYTGSAIGQHWMYQGKHVLIVFDDLS
                     KQAEAYRAVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDDLGAGSMTGLPIIETK
                     AGDVSAYIPTNVISITDGQIYLQSDLFNSNVRPAIDVGVSVSRVGGAAQIKAMKQVSG
                     RLKLDLAQFRALEAFAMFASDLDPASRAQLARGGRLVELLKQKQSNPYPVEEQVVSIW
                     LGTTGKLDDIAVEDVQRFETEFLDYLRRENKGILDTIRETTKFEDDTEKSLEGAVDAF
                     KKQFDGSGSHNVPVGHEPDPGHLEHEIDQEQIVRQR"
     gene            complement(3500..4309)
                     /locus_tag="PKU75_14250"
     CDS             complement(3500..4309)
                     /locus_tag="PKU75_14250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HPF82087.1"
                     /translation="MRGSSRAAAAAGMDALREVVGSVDAADLAEQLFAAGSTIDGNAT
                     LRRALTDPSREGEAKDGLARRLFGGKVSDQATHVLAVLAGQRWADERDLSDTIENFGV
                     AALAESADRAGRLQQVEDELFRFERIVDGTPQLRDALTNRQGNPAGKAELVERLLDGK
                     SAPETVRLAKQAVGKRSDKRLDAILESYLKLIADRRGQLTAVVTSAQEISGEERQRLA
                     GALERLYGRPIQVQSIVDPQIVGGLKVQVGDEVIDGTIATKLEGARRHFGG"
     gene            complement(4309..4821)
                     /locus_tag="PKU75_14255"
     CDS             complement(4309..4821)
                     /locus_tag="PKU75_14255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPF82088.1"
                     /translation="MLPLWGELIFGIIAFAILYWVVAKKVVPNLEKAYAARAAAIEGG
                     MAQAEEAQAAADAAKAQYEAQLKDARTEASKIREDARVQGTQIVNEMRGQAQAEATRI
                     TDAAHRQIEAERSQAAASLRGEVGRLSTDLASKIVGESLHDEARQKGIVDRFLAELES
                     GDLKPQKVES"
     gene            complement(4958..5164)
                     /gene="atpE"
                     /locus_tag="PKU75_14260"
     CDS             complement(4958..5164)
                     /gene="atpE"
                     /locus_tag="PKU75_14260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPF82089.1"
                     /translation="MQGNIAILGYGLSAIGPGIGIGLIFAAFINGVARQPEARGMLQG
                     IAILGFALAEALAIFGLVLAFVFR"
     gene            complement(5288..>6005)
                     /gene="atpB"
                     /locus_tag="PKU75_14265"
     CDS             complement(5288..>6005)
                     /gene="atpB"
                     /locus_tag="PKU75_14265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPF82090.1"
                     /translation="IFGSGDWTFTRPMLMMALSVLILGVLLVTLTKKAAIVPGKGQWM
                     VEQAYDFVRNGIARDIIGSKDFLKYTPLLFSLFTLILLNNLFGIIPPIQYPTMSRIGF
                     PIALTLIVYAVYHIAGMRRKGVGHYLSGMIPKGLPKPIYALIIPLEYATYFIIRPLTL
                     ALRLFGNMFAGHMLLLLFIMGGEYMLLHGGPGLKVVSVGSFLMALIFTAFEALIQFLQ
                     AYIFTLLAATYISDAISEEH"
BASE COUNT          912 a         1894 c         2141 g         1058 t
ORIGIN      
        1 tgaacagcag cacgtcctgc ttctgcacat cgcggaagta ctccgccatc gtcagcgccg
       61 agagggcgac gcgcagacgc gttcccggcg gctcgtccat ctgaccgaag acgagggcgg
      121 tctggccgag aacgccggcc tcctccatct cgaccatcag gtcgttgccc tcacgggtgc
      181 gctcgccgac accggcgaac accgacacac cgccgtggtc acgggcgacc cgggcgatca
      241 tctcctggat gagcacggtc ttgccgacgc cggcaccacc gaacagaccg atcttgccgc
      301 cctgcacata cggggtcagc aggtcgatga ccttgatgcc ggtctcgaac atgacggtct
      361 tggactcgag ctggtcgaag gccggtgccg ggcggtggat gccccaccgc tcctgcacat
      421 cgagggtctc gccctcctcg aggttcaggc acacgcccgt ggtgttgaag acgtggccga
      481 gggtcacatc gccgacgggc acggtgatcg ggccgccgct gtcctgcacg gccgtgccgc
      541 gcaccaggcc gtcggtcggc tgcagggaga tggcgcgcac catgttgtcg ccgatgtgct
      601 gggcgacctc gaggttgagg gtcttggtgc cctcgccccc accctcaccg gcgaggctga
      661 cctcggtggt gagcaggttg tactggtccg gcatggcgtc ggcggggaac tccacgtcga
      721 cgaccgggcc gatgatgcgg gagatgcggc cgacgccacc ctgctgggtg tcctgggccg
      781 aatcggtgac agtcgcggtc atatctggtt tcctcacttg ctctcggcca gggcgctggc
      841 accgccgacg atttcgctga tctcttgggt gatctccgcc tggcgcgctt ggttggccag
      901 ccgggtgtag gtgttgatga ggttctcggc gttgtccgtg gccgacttca tcgcgcgctg
      961 gcgggcggcc agctcggacg tcgcggcgct gagcagacag gtccagatgc gcgcggtgac
     1021 atagcgcggc agcatcgtgt gcagaacctc ttcggcgttg ggctcgaact ggtagagcgg
     1081 caggatgtcc ttgacgccgt cgtggttcgc gtcctcgggg gcgtcgacga cctcgatcgg
     1141 cagcagccgc agcacgcggg cttcctggtt gaccatcgtg ttgaagcgcg tgtagacgac
     1201 gtggatctcg tcgaggccgc cctcctcggt cggccgcagg aagtcctcga tcagccgctc
     1261 ggagatctct cgagcccgct cgacctgggg cttctcggtg ttaccggtcc actccgcggc
     1321 gtagtcgcgg ccgcggaact tgtagtagga caccgccttg cgcccgacca gatagggaac
     1381 gacctctttg ccgtccgcgc gcagctcccc gatgagacgc tcgctctcct tcatcgttcc
     1441 cgaggcgtag gcaccggcca gaccgcggtc gctggtcagg atgagcacac cggcccgcgt
     1501 ggggttctcg acctccgtgg tcatcgggtg gtcgtagttg ctgtgcgtgg cgagtgccga
     1561 cagcgccgtg gtcagcgcgt gcgtgtaggg cgcggactcg aggacggccg tgcgggcctt
     1621 gacgacacgc gaagccgcca tgagttccat tgcccgggtg atcttcttcg tcgccgacac
     1681 cgagcggatg cgctgccggt agacccggat ctgcgctccc ataagttctg cctctctgtg
     1741 gtgggaggtc tgatcgcgtc agcgcgtcag cgctggcgga cgatctgctc ttggtcgatc
     1801 tcgtgctcga ggtggcccgg gtccggctcg tgaccgaccg gcacattgtg cgaaccggaa
     1861 ccgtcgaact gcttcttgaa cgcatcgaca gcaccctcga gggacttctc ggtgtcgtcc
     1921 tcgaacttgg tcgtctcgcg gatcgtgtcg aggatgccct tgttctcgcg gcgcaggtag
     1981 tcgaggaact cggtctcgaa acgctggacg tcctcgaccg cgatgtcgtc gagcttgccc
     2041 gtggtgccga gccagatcga gacgacctgc tcctccaccg ggtaggggtt ggactgcttc
     2101 tgcttgagca gctccaccag gcgaccacca cgcgcgagct gggcacgcga ggccgggtcg
     2161 aggtcggagg cgaacatggc gaaggcctcg agcgcacgga actgggccag gtcgagcttg
     2221 agccgaccgg agacctgctt catggccttg atctgcgcgg caccaccgac acgggagacg
     2281 gagacaccca cgtcgatcgc cgggcggacg ttggagttga acaggtcgga ctgcagatag
     2341 atctgcccgt cggtgatgga gatgacgttg gtcgggatat aggccgagac gtcaccggcc
     2401 ttggtctcga tgatcggcag acccgtcatc gacccggcgc ccaggtcgtc ggacagcttc
     2461 gcgcagcgct cgagcagccg gctgtgcagg tagaagacgt cgccggggta ggcctcgcgg
     2521 cccggcgggc ggcgcagcag cagcgacacg gcgcggtagg cctcggcctg cttggacagg
     2581 tcgtcgaaga cgatcaggac gtgcttgccc tggtacatcc agtgctggcc gatggccgag
     2641 ccggtgtagg gcgcgaggta cttgaagccg gccgagtcgg acgcgggcgc ggcgacgatc
     2701 gtggtgtatt ccagcgcgcc ggcgtcctcg agggtgccac gcacggaggc gatggtcgag
     2761 cccttctggc cgatggcgac gtagatgcag cgcacctgct tggtcgggtc accggtgtcc
     2821 cagaattcct tctggttgat gatggtgtcg accgcgacgg tggtcttgcc ggtctgccgg
     2881 tcaccgatga tcagctgacg ctggccacga ccgatggggg tcatggcgtc gacggccttc
     2941 aggccggtct gcagcggctc gtgcaccgac ttgcgggaga cgacgttggg ggcctgcagc
     3001 tccagggcgc ggcgcccttc gtcggcgata tcgccgaggc cgtcgatcgg ctggccgagc
     3061 gggttgacca cgcggccgag gaaggcgtcg cccaccggca ccgagaggac ctcaccggtg
     3121 cgcttgacgg tctggccttc ttcgatcttg gagaagtcac cgaggatgac gacaccgatc
     3181 tcgtggacgt cgaggttgag cgcgatgccc agggtgccgt cctcgaactg gagcagttcg
     3241 ttcgtcatcg ccgaaggcag gccctcgacg tgtgcgatgc cgtcaccggc gtcgatgacg
     3301 cggccgacct cctcccggga ggcctggccg ggctggtagg actggacata ggcgtccagc
     3361 gcgtcccgga tctcctcggg acggatcgaa agctccgtca tctcttcttc tctcctttgg
     3421 cgtccgcggt ggctgcgggg ctgtctgtat gtcgcttgtg tgactgtggt cggccggggg
     3481 ccgtccgcgg ggtgtggggt cagccgccga agtggcgacg ggccccttcg agcttggtgg
     3541 cgatggtgcc gtcgatgacc tcgtcgccga cctggacctt caggccgccg acgatctggg
     3601 ggtcgacgat cgactggacc tggatcgggc ggccgtagag ccgctcgagc gcgccggcga
     3661 gccgctgccg ctcctcgccg ctgatctcct gggcggaggt gacgacggcg gtcagctggc
     3721 cgcgccggtc ggcgatgagc ttgaggtagg actcgaggat ggcgtcgagg cgcttgtcgc
     3781 tgcgcttgcc gacggcctgc ttggcgaggc gaaccgtctc cggtgcggac ttgccgtcga
     3841 gcagccgctc cacgagttcg gccttgcccg cagggttgcc ctggcggttg gtcaaggcgt
     3901 cccgcagttg cggggtgccg tcgacgatgc gctcgaaccg gaagagctcg tcctcgacct
     3961 gctgcagccg gccggctcgg tcggcactct cggcgagggc cgcgacaccg aagttctcga
     4021 tggtgtcgct caggtcccgc tcgtccgccc agcgctggcc cgccaggacg gcgagcacgt
     4081 gggtggcctg gtcggagacc ttgccgccga agagccggcg cgccaggccg tccttggcct
     4141 cgccctcgcg ggaggggtcg gtcagggcgc ggcgcagggt cgcgttgccg tcgatggtcg
     4201 aaccggccgc gaaaagctgc tcggccaggt cggcggcgtc gacggaaccg acgacctcgc
     4261 gcagggcgtc catccccgcc gcggcggcag cacgagagct gccgcgcatc aggattcgac
     4321 cttctgcggc ttcaggtcgc cggattcgag ttcggcgagg aagcggtcga cgatgccctt
     4381 ctggcgggcc tcgtcgtgga gggactcccc gacgatcttg ctggccaggt cggtcgagag
     4441 gcgacccacc tcaccgcgca agctggcggc ggcctgcgag cgctcggcct cgatctgccg
     4501 gtgggcggcg tcggtgatcc gggtggcctc ggcctgcgcc tggccacgca tctcgttgac
     4561 gatctgcgtg ccctggacgc gagcatcctc gcggatcttg ctcgcctcag tgcgggcgtc
     4621 cttgagctgg gcctcgtact gcgccttggc ggcatcggcg gcagcctgcg cttcctcggc
     4681 ctgggccatg ccaccctcga tggcggccgc gcgggcggca tacgccttct cgaggttggg
     4741 cacgaccttc ttggcgacga cccagtagag gatcgcgaag gcgatgatcc cgaagatcag
     4801 ctccccccac aggggcagga tcggggtccg cccggcgacc gggtcgcctc ccgcggttcc
     4861 ctcggtttcc gcgacgaagg tcaccagcgg tgcgagacct gtcaagtgca cggcgttctc
     4921 ctagtgctcg gtgcgaacga atcggtggtg ctgcggatca gcggaagacg aacgcgagaa
     4981 cgagaccgaa gatggcgagc gcctcggcga gggcgaagcc gaggatggcg atgccctgga
     5041 gcatgccgcg ggcctcgggc tggcgggcga cgccgttgat gaaggcggcg aagatcaggc
     5101 cgatgccgat accggggccg atagccgaca ggccgtagcc gaggatcgcg atattgcctt
     5161 gcatgaaagg tcctcttcca tttctgtgtg atccgacacc ggcactgcgc cgagcggaag
     5221 tctctgcggg ttattcagtt gtgatttgcg gtcagtggga cccgccctcc gaccggacag
     5281 cttggggtca gtgctcctcg gagatcgcgt ccgagatgta ggtcgcggcg aggagggtga
     5341 agatgtaggc ctgcaggaac tggatcaggg cttcgaaggc ggtgaagatc agcgccatca
     5401 ggaacgatcc gacggagacg accttcaggc ccggcccgcc gtggagcaac atgtactcgc
     5461 cacccatgat gaacagcagc agcagcatgt ggccggcgaa catattgccg aagagtcgca
     5521 aggccagggt gagcggccgg atgatgaagt aggtcgcgta ctccagcggg atgatcagcg
     5581 catagatcgg cttgggcagg cccttgggga tcatgccgct gaggtagtgg ccgacgcctt
     5641 ttcgccgcat gccggcgatg tgatacacgg catacacgat gagagtcagg gcgatcggga
     5701 agccgatgcg gctcatcgtc gggtactgga tcggcgggat gatgccgaag aggttgttga
     5761 gcaggatcag cgtgaagagg ctgaagagaa gcggcgtgta cttcaggaag tccttggacc
     5821 cgatgatgtc gcgggcgatc ccgtttcgga cgaagtcgta ggcctgctcg accatccact
     5881 gccccttgcc cgggacgatc gccgccttct tcgtcaaggt caccagcaaa acgcccagga
     5941 tgagcaccga aagggccatc atcagcatgg gccgggtgaa ggtccaatca ccactgccga
     6001 agatc
//
LOCUS       DAOPJG010000195         5995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14207_length_5995_cov_4.711111, whole genome shotgun sequence.
ACCESSION   DAOPJG010000195 DAOPJG010000000
VERSION     DAOPJG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14207_length_5995_cov_4.711111"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..902
                     /locus_tag="PKU75_14270"
     CDS             189..902
                     /locus_tag="PKU75_14270"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPF82091.1"
                     /translation="MHTGGGGMSAPSGRADAAAVLIDLDGPITRLFPSPAHIDLARAV
                     HARVAHDPGAPPTAGEDHVQVLRRIASATPHLLAETERFAARAEMAAARRATPAPGAV
                     EFIRAAAEAGYGVAVVSNNAAPAVAATLTSVGVADWVAVVAARHPGNISRLKPHPDLV
                     DEALHALGVSADHAILLGDTVSDMRAALAAGVRAIGVTADDDRASQLRAGGASMVLRD
                     LGEAVGLLDPSPARVGERP"
     gene            899..2257
                     /gene="ribA"
                     /locus_tag="PKU75_14275"
     CDS             899..2257
                     /gene="ribA"
                     /locus_tag="PKU75_14275"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HPF82092.1"
                     /translation="MSSAGSVGDPMPVMRVAETVLPTSHGSFRMVGYRGAGGDEHVTL
                     SIGVEDGVPPATPPLVRLHSECLTGDALGSQRCDCGRQLDAALARIAGEGIGVLVYVR
                     GHEGRGIGLLEKLKAYRLQDAGADTVDANLLLGHPSDAREYDEAAAILLDLGVTDVRL
                     MSSNPAKDEALRALGIQVAERTGMFVPDVPHSVAYLATKRARMGHDTPPASDGSAWPE
                     LVAGRVPVSVHTAVDRDLVDRYGPLVADGAKQVYAQMAQSLDGFIATRDGDGAQLSGP
                     EDHEHLHRLRALADAVVVGAGTVTADDPALTVREVPGPNPLRVVIDPHSRIPRGARLL
                     VDPEAPTLWLTGADDDAEPAPHVTRIPLSPKEFEPGNLVALLRRRGVSRILVEGGGST
                     VSRFLDAGCLDRLFLTSVPVLLGDGIPGVRGRPATTVRSAPRLASRRFDLGADTCTEI
                     VF"
     gene            complement(2207..3193)
                     /locus_tag="PKU75_14280"
     CDS             complement(2207..3193)
                     /locus_tag="PKU75_14280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPF82093.1"
                     /translation="MSSWPDDRPRVVACPVRPGEPRTASTLRSALRAHRVSIVDGLLG
                     CEHPGELAGAEAEGHRIALLVHMPRPEEPGLQPDDRSRLATLESAAVHAASAVIVPSR
                     WAAHDLETRYGRTDLHVAVPGSERAPLAPAHPIPVMLQLGAIGPLKNQLLTARAALAA
                     PRSSFVVQFVGPVVDESYAAELASLIRPLGRWAGVHPAVTGEDRDRVLARADLMISVS
                     RHESYGLTVTEGLARGIPALVGRGTGAQEALGAGGGLPGACVDTHDPAEMTAALTAFA
                     DDPGTRDRWRAAATVARDRLPSWAPTARRISEVCESLEHDFGARVRAEIEPP"
     gene            complement(3274..3654)
                     /locus_tag="PKU75_14285"
     CDS             complement(3274..3654)
                     /locus_tag="PKU75_14285"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPF82094.1"
                     /translation="MFRLTVRDRLMVAHSLPDPFFGPAQQLHGATFVVEATFERPHLD
                     EHAVVVDIGAAGDHLAEVLAPMNYRNLDDLPQFACILTTTERLAQHIAEQLRDRMDLR
                     GLTAIEILLREHPDAWASHRIELE"
     gene            complement(3654..4652)
                     /locus_tag="PKU75_14290"
     CDS             complement(3654..4652)
                     /locus_tag="PKU75_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HPF82095.1"
                     /translation="MEHGEPPSTARAYWTVAAGRGEIREEPLRSPGPGEVLVRTVMSG
                     VSRGTELLVHRHEVPEAVAELMRAPHQVGDLPGPVKYGYLAVGVVEAGPPDLLGARVF
                     CLHPHQDWFVVPAADIVRVPDGVPSARAVLAGTVETAVNALWDAGPRIGDRIAVIGAG
                     MVGLSIALLLRRHPLARLEVVETDAGRRGLVAGLGLTALDPAQAHDECDLVFHTSASS
                     PGLATALELLGVEGEVIELSWFGERTTEVPLGGPFHVRRLSVRSSQVGRIAPARSARR
                     TFADRLGIALDALQDNAFDALLSDPIPFDRLPAAMTRMADGDPEVICQLVDYHQET"
     gene            complement(4639..5283)
                     /locus_tag="PKU75_14295"
     CDS             complement(4639..5283)
                     /locus_tag="PKU75_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPF82096.1"
                     /translation="MNPANQVTTARAAGALGCAAIVVLDLAGRLGEGSWWLAVVAGPT
                     LLLDLLDGHVARRTRSVTELGTRFDMEVDAAMVAILSASAVAACPPALLVGASRYVLA
                     LGQWVRPAWRGDVAPRRSRRRVAAAVAVLLWTATLPVLPAAAIWACILVALVLLAWSF
                     GTQVIELERGSAAVAARGNASRSPDLRARRVHASTPAPAAPTAPTRREHLRGAW"
     gene            complement(5304..>5995)
                     /locus_tag="PKU75_14300"
     CDS             complement(5304..>5995)
                     /locus_tag="PKU75_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82097.1"
                     /translation="IDPLAGVEALTRELLDAGVRELDAWARETFEVGAPQWSWSAQDY
                     RLTTQVPSIGPIPGTKGGLLVATGCDKWGMANAAAAGHILTGDITGNPPSFVPALRSG
                     LPGVADVVDSAITIAGIGMSWVGARAALAVGGGRVGGGKVAGGNPQTPAHGKGHVEGG
                     PVHPVGVATVDGKTCRVSAVCPHLGGILAWERRRDVVGLPFARFAIRGRWPSCRGSGD
                     QRPDRPRRLPR"
BASE COUNT          858 a         2109 c         2175 g          853 t
ORIGIN      
        1 ttggtgtccg gtgagcgggt cgcggggcag gttctcgccg acgtgggggc gccgcagtag
       61 caagggatgt ggggtgagac ggcccgccat ggctgcgccc tcgccgatcc cgtgatgccg
      121 gcgcccggcg gcaaatcgcc accccattgg atgggtcacc gcacgcctcc ccggcatagg
      181 ctcgaacgat gcacacaggc gggggcggca tgagcgcacc atcgggacgc gccgatgccg
      241 cggcggttct catcgacctg gacggcccga tcacccggct gtttcccagt ccggcccaca
      301 tcgaccttgc ccgcgccgtc cacgcccgcg tcgcgcacga cccaggcgcc ccgccgacgg
      361 ccggcgagga ccacgtccag gtgctgcgcc ggatcgccag tgcgacgccg cacctgctgg
      421 cggagacaga gaggttcgcc gcccgcgcgg agatggcggc cgctcgccga gcgacgccgg
      481 ctccaggggc agtcgagttc atccgggcgg ccgccgaggc cgggtatggc gtggcggtgg
      541 tgtcgaacaa cgccgcgccg gccgtcgccg cgacgctgac gagcgtgggg gttgcggatt
      601 gggtggctgt ggtcgccgcc cgccatcccg gcaatatctc tcgcctcaag ccccacccgg
      661 acctggtcga cgaggcactt cacgccctcg gcgtcagcgc ggaccacgcg atcctcctcg
      721 gcgacacggt cagcgacatg cgcgccgcgc tggccgccgg tgtgcgcgcg atcggcgtca
      781 ccgccgatga cgatcgcgcg tcgcagttgc gagcaggcgg agcgagcatg gtgctgcgag
      841 atctcgggga agcggtcggg ctgctcgatc cgagtccggc gagggttggt gaacggccgt
      901 gagcagcgcc ggttccgtcg gtgacccgat gccggtgatg agggtggccg agaccgtcct
      961 gcccacctcg catggttcct tccgcatggt cggctaccgc ggggccggcg gggatgagca
     1021 cgtcacgctc tcgatcgggg tcgaggacgg cgttcccccg gcaacgccgc cgctggtgcg
     1081 tctgcactcc gaatgtctga ccggggacgc gctcgggtcc cagcggtgtg actgcggccg
     1141 gcagttggat gcggccctgg cccggatcgc gggcgagggg atcggcgtgc tcgtctacgt
     1201 ccgggggcac gaagggcgcg ggatcggtct gctcgagaag ctgaaggcct accggttgca
     1261 ggacgccggt gccgacacgg tggacgccaa cctcctcctg ggccatccga gcgatgcccg
     1321 agagtatgac gaggccgccg cgatcctcct cgacctcggc gtgaccgatg tgcgactcat
     1381 gagttcgaac ccggcgaagg acgaggcctt gcgcgcgttg ggaatccagg tcgcggagcg
     1441 caccggcatg ttcgtccccg acgtgccgca cagcgtcgcc tatctcgcca ccaaacgggc
     1501 ccggatgggc cacgacaccc ctccggcgtc ggacggctcg gcgtggcccg agttggtggc
     1561 ggggcgggtg cccgtctcgg tccacaccgc cgtcgaccgg gacctcgtcg accgctacgg
     1621 tcccctcgtc gccgacggtg ccaagcaggt ctatgcgcag atggcgcaga gtctcgacgg
     1681 tttcatcgcg acccgcgacg gtgacggggc gcagctgtcc ggcccggagg accacgagca
     1741 cctgcaccgg cttcgcgcgc tcgccgatgc cgtcgtcgtg ggggctggca cggtcaccgc
     1801 cgacgacccc gccttgacgg tccgggaggt gccggggccg aatccgctgc gcgtcgtcat
     1861 cgacccgcac tcccggatcc cccgcggcgc ccgcctcctc gtcgacccgg aagctcccac
     1921 gctgtggctg acgggcgctg atgatgacgc cgaaccagcc ccccacgtca ctcggatccc
     1981 tttgtcgccc aaggaattcg aaccgggcaa cctggttgct ctgctgcgtc gtcgtggcgt
     2041 ctcccggatt ctcgtcgaag gcgggggcag cactgtctcc cgcttcctcg acgcgggatg
     2101 cctcgaccgg ctcttcctca ccagcgtgcc ggtgctcctc ggggacggca tccccggagt
     2161 gcgcggccgc ccggcgacca cggtccggtc ggctccacgg ctcgcgtcac ggcggttcga
     2221 tctcggcgcg gacacgtgca ccgaaatcgt gttctagcga ttcgcagacc tccgagatcc
     2281 ttcgggccgt cggcgcccac gacgggagcc ggtcccgcgc caccgtggcc gccgcccgcc
     2341 accggtcccg tgtccccggg tcgtcggcga atgccgtgag agcggcggtc atctcggccg
     2401 ggtcgtgcgt gtcgacgcag gcgccgggca gtccgccgcc ggcgccgaga gcctcttgcg
     2461 cgccggtgcc tctgcccacg agggccggta tgccgcgcgc caacccctcc gtgaccgtca
     2521 gtccgtagct ctcgtgccgg gacacgctga tcatcaggtc ggcccgtgcc aacacccggt
     2581 cgcgatcctc tcccgtgacc gccgggtgca ccccggccca ccggcccagg ggtctgatga
     2641 gcgaagccag ctcggcggca tacgactcgt cgaccacggg accgacgaac tgcacgacga
     2701 aggacgagcg tggcgcggcg agggcggcgc gtgcggtgag cagctggttc ttgagcgggc
     2761 cgatcgcgcc gagctgcagc atgaccggga tgggatgcgc cggcgcgagc ggggcgcgct
     2821 ccgagccggg aaccgcgacg tgcagatcgg ttctgccgta tcgggtttcg agatcatgtg
     2881 ccgcccaccg gctcggcacg atcacggcgg aggcggcgtg gacggcggct gactcgagcg
     2941 tcgccaggcg actcctgtcg tccggctgca gtcccggctc ttcgggacgc ggcatgtgca
     3001 cgaggagggc aatgcggtgt ccctcggctt cggctccggc gagttctccc gggtgttcgc
     3061 acccgagcag accgtcgacg atgctgaccc ggtgggcccg cagcgccgag cgcagggtgc
     3121 ttgcggtgcg gggctcgccc ggtcggaccg ggcacgccac gactctcggg cgatcatcgg
     3181 gccaggagct catgacggag tcgttgtaat ggcttcctcc ggacggccgg cccagctccg
     3241 gcacgacgaa gccgattgcg ttcgcgtcca ccgctattcg agttcgatgc ggtggctggc
     3301 ccacgcatcc gggtgctccc gcaataggat ctcgatcgcg gtgaggccgc gcagatccat
     3361 ccggtcgcgc agctgttcgg cgatgtgttg agcgagccgc tcagtcgtcg tcaggatgca
     3421 ggcgaactgg ggcaggtcgt cgagattgcg atagttcatc ggtgccagga cctccgcgag
     3481 atggtctcct gctgctccga tgtcgacgac gacggcgtgc tcgtcgaggt gcggtcgttc
     3541 gaacgtggcc tcgacgacga aggtcgcacc gtggagttgc tgggccggac cgaagaaggg
     3601 atcgggcagg ctgtgagcga ccatgagccg gtcgcggacg gtcaatcgga acatcaggtc
     3661 tcctggtggt agtcgacgag ttggcagatg acttctgggt ccccgtcggc catgcgggtc
     3721 atggccgcgg gcagtcggtc gaaaggtatg gggtcgctga gtagggcatc gaaggcgttg
     3781 tcctgcaacg catccagggc gataccgagg cgatcggcga acgtgcggcg cgcagaacgc
     3841 gccggcgcga tccgccccac ctggctgctg cgaaccgaca ggcgacgcac gtggaagggg
     3901 ccgccgagcg ggacctccgt ggtgcgctcg ccgaaccagc tcagctcgat cacctcgccc
     3961 tcgacaccga gcaactcgag cgcggtagcc aggccgggtg atgacgccga ggtgtggaag
     4021 acgaggtcgc actcgtcgtg ggcctgagcc gggtcgagcg ccgtcagacc cagaccggcc
     4081 accaggccgc ggcggccggc atcggtctct accacctcca ggcgcgccaa cggatgccgc
     4141 cgcaggagca gggcgatgct cagcccgacc atgccggcgc cgatgaccgc gatccggtcg
     4201 ccgatgcgag gtccggcgtc ccacagggcg ttgacggctg tctcgacggt gccggcgagc
     4261 acggcacggg cgctcgggac cccgtcgggc acgcggacga tgtcggcggc cgggacgacg
     4321 aaccaatcct ggtgggggtg gaggcaaaag acacgagctc cgaggagatc gggcgggccg
     4381 gcctcgacga caccgaccgc cagataaccg tatttgaccg ggccgggcag gtcgcccacc
     4441 tgatgcgggg cgcgcatgag ttcggccacg gcctcgggga cctcatgccg gtggaccagc
     4501 agttcggtgc cccggctgac gccgctcatc acggttcgca ccagcacctc gcctggtccc
     4561 ggggaacgca ggggttcctc acggatctct ccgcggccgg ccgcgacggt ccaatacgcc
     4621 cgggcggtgg atggcggttc accatgctcc acgaaggtgc tcccttctcg tcggtgccgt
     4681 cggtgccgcc ggtgcgggag tcgaggcgtg gacccgacgc gcacggaggt ccggagatcg
     4741 ggacgcgttt ccccgggcgg cgacggcagc gctcccgcgt tcgagttcga tcacctgggt
     4801 gccgaaggac cacgcgagca ggaccagggc cacgaggatg cacgcccaga tcgccgccgc
     4861 cgggagcacc ggcagcgtgg cggtccagag caacaccgcg accgccgcag ccacgcgtcg
     4921 tcggctgcgt cggggcgcga cgtcgccccg ccaggccggg cgcacccact ggccgagcgc
     4981 cagcacgtac cgcgaggcgc cgacgagcag ggcgggcggg caggcggcga cggcgcttgc
     5041 cgagaggatc gccaccatgg cggcgtctac ttccatgtcg aagcgcgttc cgagctcggt
     5101 caccgaccgc gtccggcgag ccacgtgacc gtcgaggagg tcgagcagca aggtcgggcc
     5161 cgcgaccacg gccagccacc acgagccctc cccgagccgg ccagcaaggt ccagcacgac
     5221 gatggcagcg cacccgagcg ccccggcagc gcgcgccgtc gtcacctggt tcgcgggatt
     5281 caccggctca cccggccggc tcctcagcga gggagtcgtc gaggacggtc aggtcgctgg
     5341 tcgccggacc ctcgacaaga gggccatcgg cctcgaatcg cgaaccgtgc aaagggcagt
     5401 cccacgacat ctcggcgtcg ttcccacgcg agaatcccgc ccagatgcgg gcagacggca
     5461 ctgacccggc acgtcttccc gtcgaccgtg gcgaccccga ccggatgcac ggggccgccc
     5521 tcgacgtgtc ccttgccgtg ggccggtgtc tgggggttgc cgcctgcgac cttgccaccg
     5581 ccgaccctgc cgccgccgac cgcgagagcg gctcgggcgc cgacccagga catcccgatg
     5641 ccggcgatcg tgatggcgct gtcgacgaca tcggccaccc ctggcagacc ggaacgcaac
     5701 gccggcacga acgagggcgg gttgccggtg atatcgccgg tgaggatgtg gccggctgcc
     5761 gccgcgttgg ccatccccca tttgtcgcac ccggtggcca cgagcagccc gcccttggtg
     5821 ccgggaatgg gaccgatgct gggcacttgg gtggtgaggc ggtagtcctg ggctgaccag
     5881 gaccattggg gcgcgccgac ctcgaaggtc tcgcgggccc acgcgtcgag ttcgcggacg
     5941 ccggcgtcga gaagttcgcg ggtcagcgcc tcgacgcccg caagcgggtc gatct
//
LOCUS       DAOPJG010000196         5909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14486_length_5909_cov_6.171507, whole genome shotgun sequence.
ACCESSION   DAOPJG010000196 DAOPJG010000000
VERSION     DAOPJG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14486_length_5909_cov_6.171507"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PKU75_14305"
     CDS             <1..355
                     /locus_tag="PKU75_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPF82098.1"
                     /translation="VRITHVPTGVVVSCQNEKSQLQNKESALRVLRARLHQMAMDEAA
                     AAASDARKSQVRTVDRSERIRTYNFPENRISDHRTGYKAYNLEIFLGGEMDPVVEAAM
                     AADEAARLAALGEPT"
     gene            435..1343
                     /gene="prmC"
                     /locus_tag="PKU75_14310"
     CDS             435..1343
                     /gene="prmC"
                     /locus_tag="PKU75_14310"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPF82099.1"
                     /translation="MSGLRQVMRDAAARLAEAGVPSPDADVVELVSFVLRIEPSEVRR
                     RMIVGAELTAAQEDRLGDLIEGRSHRIPLQHLTGRAYFRHLTLQVGPGVFVPRPETEM
                     VAGLAIEAARAAGPAPVVVDLGTGSGAIALAVADEVPAARVYAVELSDLAVAWARRNV
                     EALGSPVELRQGEAAAAFPELEGGVDVVVSNPPYIPAGQVPVDPEVRDHDPELALYGG
                     SADGLTIPLAVAARAARLLRPGGVFVMEHADTQGEALVRVLRRESDSTPSRCHRWVDV
                     VDHLDLTGRPRAVVARRAAGEGQAGC"
     gene            1451..1606
                     /locus_tag="PKU75_14315"
     CDS             1451..1606
                     /locus_tag="PKU75_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82100.1"
                     /translation="MSNFIKNFLKDLRPTKADIELIAAIKAYPARGGSAVVVLPHASR
                     THQDVTR"
     gene            2107..2493
                     /locus_tag="PKU75_14320"
     CDS             2107..2493
                     /locus_tag="PKU75_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82101.1"
                     /translation="MIVNEIRAVDRFGRPQLSGDVAGLDVRRGEELWRVPGGRLQARG
                     TSDDLVLAGPLVEFGGRVQVDRRRYRRHASPRRGRRRVAVDPGGGCLCRWQAPADLHR
                     LVLAAEPDNLDYRADSGPVRIVSLTR"
     assembly_gap    2507..2606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2607..3294)
                     /locus_tag="PKU75_14325"
     CDS             complement(<2607..3294)
                     /locus_tag="PKU75_14325"
                     /inference="COORDINATES: protein motif:HMM:NF025428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HPF82102.1"
                     /translation="MDDPRQLYADAGLALPPPTDRGEVRPGMRVLSLVVPETESTLEV
                     WERLRDLHPHTGYWPIVVGEGLWESTIFEFAGPGSAQPYAAGDGRAWFEAKYAERFGE
                     EGPIRGQAEPVPGTDTWDDLLDVTLGEATEIALVPAAYGWEAPSVLGWSGAVNYDIDE
                     SEHATVLRRWSGQWGLEVVGLSLDILSARIARPATTPEERLARAMEIYLYCPDAVDQG
                     VGSIDALAAGT"
     gene            complement(3402..4646)
                     /locus_tag="PKU75_14330"
     CDS             complement(3402..4646)
                     /locus_tag="PKU75_14330"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HPF82103.1"
                     /translation="MSATHPDPGNPESAEPDPGRPDPAVTLRALIAGVTSAGEEGHVL
                     DLLHSLDDEQLSSAVTGVDAGDLFGSVDDRVFGPDRRTALRELFRSRASGLSVAARAS
                     LLYGLQRGHTSDADEELMRDLFLAVGGRELTEVKNAINMRTDAHDLEGLVFEDIDSDE
                     IRDAILAHFAAEAPAVGIAGTKVLSDIDDTAFSRIHEKRYPKGEVIPGILAVYEALDL
                     GTSEPPFSRGDLAFVTARPDDALSLIEGATRESLAKAGIASVNVIGGSFLALRSKADM
                     AAKKGANIDNYSRLFPEYRIVFIGDSGQGDPLTGETILQQHPESVATVIIHDVVATSP
                     DERAEWAAKGLHFVDTYVGAAPILREMGLICPKQVARIAEETSAALDAIAWESPEQEA
                     AARALVDRDTKAAAPAMVDDRP"
     gene            complement(4685..>5909)
                     /locus_tag="PKU75_14335"
     CDS             complement(4685..>5909)
                     /locus_tag="PKU75_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPF82104.1"
                     /translation="DTTDTTTTADTTSAADTTSAADPFTDDQTANNQDADEQGADYQG
                     AGDAVTGAPGVPGHDETGHDETGHDDTNTNADDDADCGANTNADYHAHDYAGCDADDE
                     ADGRGGVGDPAGALWPGRRVRPVDPHSVRPSEFSMFWDGDRFRMRGSFDAADGALIEN
                     ATREAKDALFTAGNTEATLADAVVEIASRSLGSIDSPSRAGHYRVYVHVDTQGAWVNA
                     RGSLARHLAERLGCTATAQALWHTDAVPVSVGRAQRAIPDRTRRLVEDRDRGCAYPGC
                     TATRFVEIHHLRHWEDGGGTDYDNLLCLCPHHHSAHHAGQFTITPTGNTMVPFTFTAR
                     AGWTIKAETPPGHDNTSHDNTGNDNAGDGDGDGDGDGDTGAGNRHPRSGTTYTPPLGE
                     RADYGWISYRKRDTA"
BASE COUNT          765 a         1932 c         2155 g          957 t
ORIGIN      
        1 ggtgcggatc acccacgtgc ccacgggcgt ggtcgtctcg tgccagaacg agaagtccca
       61 gctgcagaac aaggaatcgg ccctacgcgt gctgcgcgcc cggctccacc agatggcgat
      121 ggacgaggcc gcagcggccg cctccgacgc ccgcaagtcg caggtgcgca ccgtcgaccg
      181 ttcggagcgg atccgcacct acaacttccc cgagaaccgc atcagcgacc accgcaccgg
      241 ttacaaggcc tacaacctcg agatcttcct cggtggcgag atggacccgg tggtcgaggc
      301 cgcaatggcc gccgacgagg ccgcgcggct cgctgccctc ggcgaaccca catgacgtcg
      361 agagtgcacg acgcgccgca atggcgcggc gatgacggcg cgtcgcgcac tgtcgacgtg
      421 gggcgagggc gggggtgagc ggcctgcggc aggtgatgag ggacgctgcc gcccggctcg
      481 ccgaggcggg tgtcccgtcg cccgacgccg atgtcgtcga actggtctcc ttcgttctcc
      541 gcatcgagcc gtcggaggtg cgtcgccgga tgatcgtcgg cgcggaactc actgccgcgc
      601 aggaggatcg actcggcgac ttgatcgagg ggcggagcca ccgcataccg ctgcagcacc
      661 tgaccgggcg cgcctacttc cgtcacctga ccctccaggt ggggccgggg gtcttcgtgc
      721 cgcgccccga gaccgagatg gtcgccgggc tcgcgatcga ggccgcgcgc gccgccgggc
      781 cggcgccggt ggtcgtcgac ctgggcacgg gctcgggggc gatcgcgctc gccgtcgccg
      841 acgaggtgcc ggccgcgcgg gtgtatgccg tcgagttgtc cgatctcgcc gtcgcctggg
      901 cgcggcgcaa tgtcgaggcc ctcgggtcgc ctgtggagtt gcggcagggc gaggcggccg
      961 cggcgttccc cgagttggag gggggcgtcg atgtcgtcgt gagcaacccg ccctatatcc
     1021 ctgccggcca ggtgcccgtc gaccccgagg tccgcgacca cgatcctgag ctggctctct
     1081 atggcggctc cgccgacggg ctcaccatcc cgctggccgt cgccgcccgg gccgcccgat
     1141 tgctgcgtcc cggaggggtt ttcgtgatgg agcatgccga cacccagggc gaggccctcg
     1201 tccgcgtcct ccgtcgagag agcgattcga ccccgtcgag atgtcatcgc tgggtcgacg
     1261 tggtggacca cctcgacctg accggccgcc cccgggcggt tgtcgctcgc cgagcagcgg
     1321 gggaggggca ggcgggctgc tgatcgcgcc gatgcccggg aacatactca ccgatctatg
     1381 cgtggcgctc atgattgtct tgtgtttctg ctattggacg catggctgag cgatgccgca
     1441 ccctggagtc atgagcaatt tcatcaagaa cttcctcaag gaccttcgcc cgacgaaggc
     1501 cgacatcgaa ctcattgcgg ccatcaaggc ctaccccgct cggggcggca gcgccgtcgt
     1561 cgtcctgccg cacgcctcgc gcacccacca ggacgtcacc cgctgatccg tcgcccgtcc
     1621 gtgccgcggg agcggcccgg gaaccgggtc gcgcggactt cgactcggtg acggttgccg
     1681 gagagactgt gctcgtcgcg aggaatcctt cgagcgaatg gatcctggag gcccgagaag
     1741 ccagcaaccc ggcggtgcgc tggacgcatc gcatctgcac ctatccgata cacggctaca
     1801 ccggaggccg cgccgagtcc ggcccggtgg tcgtcgattc gggtggtcga aagctggcgc
     1861 tcatcaagga cttctcgctg tgtgacgatc acggcaactg ctccaggaac tggcaacgcg
     1921 tcggcatcac ggcgttggac gtcagcacgg gcgaggtcgc gtggtccacg atggattccg
     1981 atgcgccaga ggtcatcccg gcaacaccca accgctggtc gaacgtccgg ctgctcggcg
     2041 tcaccgaggg gggtcgctgt cctggcaggc acgggctggg acggcgtcgg agtcggaggt
     2101 ggccgagtga tcgtcaacga gatccgggcc gtcgaccgtt tcggacgccc gcagctgtcc
     2161 ggcgacgtcg cggggctcga tgtccggcgg ggggaagaac tctggcgcgt gccgggagga
     2221 cgtctgcagg cccggggaac cagcgacgat ctggtgctcg ccggccccct ggttgagttc
     2281 ggcgggcggg tccaggttga tcgccgtcgc taccggcgac acgcttcgcc aaggcgaggt
     2341 cggcggcgag tggcggtgga tcccggcggt ggttgcctgt gccgatggca agcacctgcg
     2401 gacctacacc ggctggtgct cgccgccgag cccgacaacc tcgactaccg ggccgacagc
     2461 ggacccgtgc gcatcgtgtc cctgacgcgg tgaataagcc gtgtccnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnaggt gccggcggcg agggcgtcga tcgacccgac
     2641 gccctggtcg accgcatcgg ggcaatagag gtagatctcc atggcgcgcg cgaggcgctc
     2701 ctcaggcgtg gtcgccggcc gggcgattcg ggccgagagg atgtcgagcg agagaccgac
     2761 gacctccagg ccccactggc cggaccaccg acgcagcacg gtcgcgtgct cgctctcgtc
     2821 gatgtcgtag ttcaccgctc ccgaccagcc gagcacggag ggtgcctccc agccgtatgc
     2881 ggcgggcacg agcgcgatct ccgtcgcctc gccgagggtg acgtcgagca ggtcgtccca
     2941 ggtgtccgtg ccgggcacgg gttcggcttg gccgcggatc gggccttctt caccgaaccg
     3001 ctcggcatac ttcgcctcga accacgcccg cccatcgccg gcggcatacg gttgtgcaga
     3061 gcccggcccg gcgaactcga aaatcgtgct ctcccagaga ccttcgccga ccacgatcgg
     3121 ccagtagccg gtgtgagggt gtaggtcacg gagccgttcc cacacctcca gtgtcgactc
     3181 tgtctctggg acaacgagac tcagcacccg catcccgggg cgcacctcgc cacggtcggt
     3241 gggcggtggc agcgcgaggc cggcatcggc atacagctgg cgtggatcgt ccaccggcac
     3301 aacggtatcc gccaccgcga cggggtcggg tgcgaatggt ggcacaggta gcggcgcggg
     3361 cggggacgga gccggctcgc tcttggagcg gcggcggaag atcatggccg gtcgtccacc
     3421 atcgccggtg cggcggcctt ggtgtcgcgg tcgacgagcg cccgcgccgc cgcctcctgc
     3481 tcggggcttt cccacgcgat cgcgtcgagc gcggcggatg tctcctcggc gatgcgcgcg
     3541 acctgcttgg ggcagatcag gcccatctcg cgcaggatgg gggcggcccc gacgtaggtg
     3601 tcgacgaagt gcaacccctt ggccgcccac tccgcccgct cgtcggggga ggtggctacg
     3661 acgtcgtgaa tgatcactgt cgcaacggat tccgggtgct gctgcaggat cgtctcgccg
     3721 gtcagggggt cgccctggcc gctgtcgccg atgaagacga tccggtattc ggggaagagc
     3781 cggctgtagt tgtcgatatt ggcgcccttc ttggcggcca tgtcggcctt gctccgtagc
     3841 gcgaggaagc tgccgccgat gacgttgacg ctggcgatgc cggccttggc caggctttcg
     3901 cgggtcgcgc cctcgatgag gctgagggca tcgtccggac gggcggtgac gaacgcgagg
     3961 tcgccgcggg agaacggcgg ttcactggtg ccgagatcga gcgcctcgta gacggcgagg
     4021 atgccgggga tgacctcgcc cttggggtag cgcttctcgt ggatccggga gaaggctgtg
     4081 tcgtcgatat cggagagcac cttggtgccc gcgatgccga cagccggcgc ctcggcagcg
     4141 aagtgcgcga ggatcgcgtc gcggatctcg tcactgtcga tgtcctcgaa gacgaggccc
     4201 tcgaggtcgt gggcgtcggt gcgcatgttg atggcgttct tgacctcggt cagttcgcgc
     4261 ccgccgacgg cgaggaagag atcgcgcatc agttcctcgt ccgcgtcact ggtgtgcccc
     4321 cgctgcaggc cgtagagcag cgacgcgcgc gccgccaccg acagcccgct cgcgcgactg
     4381 cggaacagct cgcgcagggc ggtgcgccgg tcagggccga acacccggtc atcgacgctc
     4441 ccgaagaggt caccggcgtc cacgcccgtc acggccgagc tcagctgctc gtcgtcgaga
     4501 ctgtgcagca ggtcgaggac gtggccctcc tcgccggcgc tggtgacacc ggcgatcagc
     4561 gcgcgcaggg tgaccgcggg gtcgggtcgg ccggggtcgg gttccgcgga ctccggattg
     4621 ccgggatcag ggtgggtggc gctcacccgc ccatcttgtc agcggccggg tcgcgggttc
     4681 ccggtcaggc ggtgtcgcgt ttgcggtagc tgatccagcc gtagtcggcg cgttcgccca
     4741 ggggtggggt gtaggtggtt ccgctgcggg ggtgccggtt gccggcgccg gtgtcgccgt
     4801 cgccgtcgcc gtcgccgtcg ccgtcgccgg cgttgtcgtt gccggtgttg tcatggctgg
     4861 tgttgtcgtg gccgggtggg gtttcggctt tgatggtcca gccggcgcgg gcggtgaagg
     4921 tgaagggcac catggtgttt ccggtgggcg tgatcgtgaa ctgcccggcg tggtgcgcgc
     4981 tgtggtggtg tgggcacagg cacagcaggt tgtcgtagtc ggtgccgccg ccgtcttccc
     5041 agtgcctcag gtggtggatc tcgacgaacc gggttgcggt gcatccgggg taggcgcagc
     5101 cgcggtcgcg gtcctcgacc agtcgtcggg tgcggtcggg gatggcccgc tgggcacggc
     5161 cgaccgagac ggggacggcg tcggtgtgcc acagggcttg ggcggtcgcg gtgcagccga
     5221 ggcgttcggc caggtggcgg gccaggctgc cgcgggcgtt gacccacgcg ccttgggtgt
     5281 cgacgtggac gtagacgcgg tagtgcccgg cccggctggg gctgtcgatc gagccgaggg
     5341 atcgggaggc gatctcgacc acggcgtcgg cgagggtggc ttcggtgttg ccggcggtga
     5401 acagggcgtc cttggcctcg cgggttgcgt tctcgatcag cgccccgtcg gcggcgtcga
     5461 agctgccccg catgcggaac cggtccccgt cccagaacat cgagaactca ctcgggcgca
     5521 ccgaatgcgg atccaccggc cgcacccgcc gccccggcca cagcgccccg gccgggtcac
     5581 ccacgccgcc gcggccatcc gcctcatcgt ccgcgtcgca gcccgcgtaa tcgtgcgcgt
     5641 gatagtccgc gttggtgttc gcgccgcagt ccgcgtcatc gtccgcgttg gtgttcgtgt
     5701 cgtcgtgccc ggtctcgtcg tgcccggtct cgtcgtggcc gggcacgccc ggcgcgccgg
     5761 taaccgcgtc gccggcgcct tggtagtcgg cgccttgctc gtcggcgtct tggttgttgg
     5821 cggtttggtc gtcggtgaac gggtcagccg cgctggtggt gtcagccgca ctggtggtgt
     5881 cggcggtcgt cgtggtgtcg gtggtgtcg
//
LOCUS       DAOPJG010000197         5904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14502_length_5904_cov_7.130621, whole genome shotgun sequence.
ACCESSION   DAOPJG010000197 DAOPJG010000000
VERSION     DAOPJG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14502_length_5904_cov_7.130621"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1275
                     /locus_tag="PKU75_14340"
     CDS             103..1275
                     /locus_tag="PKU75_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82105.1"
                     /translation="MNGRRMRAAAVLASVTLAGSVVSAAPSTASDQPKERYDVSRHQT
                     TDRAAAAQVRAILDHLPADWQANRDAFLDKAGIEPSPAEDAIIAYLERATCEETELNA
                     YIETLLKGVPDDTLFVLAVLGVLSYPTFDAVIGGTKTDPRYSLETGTVGKVRQSMAAA
                     RQLWDVKNGDIDVMGMHSDMVVDRARVIRVLRVVFGVSPKDAQEAGTQIVRLIKATPA
                     LRGGTNPLFSLNAFAFTGEGDPDFGGISDRIVMGEGIIKALRWTDDIAVGPQMVLSHE
                     FGHHIQFENHAYPATESPANTRKMELMADAYGAYLDAHAKGLNFTPKQIDGTVAVSHL
                     VGDCSVDSLGHHGTPAQRARAARWGADLALKNGSNVLPSKRVQALFLTALPGILRG"
     gene            1373..2587
                     /locus_tag="PKU75_14345"
     CDS             1373..2587
                     /locus_tag="PKU75_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPF82106.1"
                     /translation="MRSYCAIYVDVGYLLASSATRVTGTSLRRGVTVDHESLIRGVIE
                     QAEQDSGMPLLRVNWYDSGGRPGGMPDIHQEHVGLLPRVKLRLGRLSQTGEQKGVDLR
                     MGLDLATYGRNRVVDVIYLISGDDDLTEAVEEAQFHGLQVVIMAVPDQRGRPHAVARN
                     LQREADNVILIDAATIDAAVKPVALPPALIPGGEDKSVESASVAPHEDAPGGGLEPSA
                     EGIPAPAASPDTTDRSAQPGEPAEPAAPHPNGARGTPAAVPSRPGPDTGTDADPGIDA
                     EAPAPPHTAAPKPSVLAGKRPSTILPPKAAPPMWTTSTGGASRGEDIGLVDIEAIDAV
                     VRQVIATWVLSATPEMLIELRKSEPFIPGDLDRTLLLDLSSRTGEYDINDASRHVMRD
                     RFWEQVKRLRLG"
     gene            2657..4012
                     /locus_tag="PKU75_14350"
     CDS             2657..4012
                     /locus_tag="PKU75_14350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPF82107.1"
                     /translation="MSRRGRTIRRIVLGVLVLAVVAVIGGYWYERPLLLTGTGYAAHN
                     ACAVRQIAGRDDPTADLPPNPLVPYLRDSTDGARTTGSLWGRLAIQKAWYTKGYGCTL
                     ADEAPDLGTATAVGVKDNPFSGAPTPTPRKAVAQALANAFGDDLPQDQRAALGTRAVV
                     VAQHGKIVGERYADGFGPSTPQLGWSMSKSVANLLVGRLVLDEVLTVDDAKLRKDWKG
                     DKAKITIDQLMRMSSGLSWDETYALGTPVTTMLYREEDMGAYAAGLDMAHKPGTYQQY
                     SSGSTNIICSVLHERTEVGADLPRQELFAKLGLSSAVWEPDASGTPVCSSYLWATPRD
                     WLTIGQFALQGGQWADQQLLPEDWMAQSTEVVKVDKTEEPGYAASWWTNKDAAGKRIS
                     EILPRDAYWAQGHDGQRLYVVPSADLVVVRLGFSPQVEPADLRADALVADLVAALPVT
                     K"
     gene            4039..4614
                     /locus_tag="PKU75_14355"
     CDS             4039..4614
                     /locus_tag="PKU75_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPF82108.1"
                     /translation="MRAIDLAEQVPTVHKETTGAEAARIVAEFRLNGLVVADDSGVPI
                     AVIPGSQLLSVVLPQYVRDDPHLAHAYDEQAADDLCAKLTTVTLGDLIDAKRLTPAQL
                     PQVEPDATVVEIVSAMSELHYPLIVVADQARFHGVVTMSRVLAAIAQAAGQDSDLIRR
                     RLDKDIIDRGRSGYLSEAGGDTARGAGEDAR"
     gene            4611..5903
                     /locus_tag="PKU75_14360"
     CDS             4611..5903
                     /locus_tag="PKU75_14360"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HPF82109.1"
                     /translation="MTLAAWLAVAVFASAYILIATEKLNRAAVALGGAAAMVAIGATR
                     GHAMFFSEETGIDWNVIFLLFGMMIIVGILKETGVFEFLAIWSAQRAQARPFRMMVML
                     ILITAVASALLDNVTTVLLVAPVTLLLCDRMALPPVPFLLAEAMASNIGGTATLIGDP
                     PNIIIASRAGLSFNDFVVNLAPIVVVLLAVFIGLAWLLWGRRLAHDPKRSSAVMALTP
                     RETITNAALLRRMSLILALVLLGFVTHAVTHIEPSVIALLGAGAAILVSRLEPDTYLE
                     DVEWETLLFFAGLFVMVGSLVHQGVIEQLARSVTSLIDGNYHVAAQGILVGSAVLSGI
                     VDNIPYVATMSPLVHGLVGPAAGLPPEEASLWWALALGADLGGNATAIGASANVVVCG
                     IAKRNGHPISFWEFTKYGLIVTAVTILCTVPYVTLRYF"
BASE COUNT          924 a         2105 c         1960 g          915 t
ORIGIN      
        1 cccgcgaccg ggtcggcgcc ggggacgatt ccgggcggga ccctgaatcg tccccggcgc
       61 cgacccggtc gcggggcgcc gaccactgca gaggggaatg gcatgaacgg acgacggatg
      121 cgcgcggcgg cagtgctcgc gagcgtgacc ctggcgggga gcgtggtctc ggccgccccc
      181 agcaccgcct cggaccagcc gaaggagcgc tacgacgtct cccggcacca gaccaccgac
      241 cgtgccgctg cggcacaggt gcgggcgatc ctggaccacc tgccggccga ctggcaggcc
      301 aaccgggacg ccttcctcga caaggccggc atcgagccca gccccgccga ggacgcgatc
      361 atcgcctatc tggagcgcgc cacctgcgag gagaccgaac tcaacgccta catcgagacc
      421 ctgctcaagg gcgttccgga cgacacgctc ttcgttctcg ccgtgctcgg cgtcctgagt
      481 taccccacct tcgacgccgt catcggcggc accaagaccg acccgcgcta ctcgctcgag
      541 acgggcaccg tcggcaaggt gcgccagtcg atggccgccg cccgccagct ttgggatgtc
      601 aagaacggcg atatcgacgt catgggcatg cacagcgaca tggtcgtcga ccgcgcccgc
      661 gtcatcaggg tgctgcgcgt cgtcttcggc gtcagcccta aggacgcgca ggaagcaggg
      721 acccagatcg tccggctcat caaggccacc ccggcgctgc gcggcggcac gaacccgctg
      781 ttcagtctca acgccttcgc cttcaccggc gaaggggatc cggacttcgg gggcatcagc
      841 gaccggatcg tcatgggcga gggcatcatc aaggcgctgc gctggaccga tgacatcgcc
      901 gtgggtccgc agatggtgct cagtcatgag ttcggccatc acatccagtt cgagaaccat
      961 gcctacccgg ccaccgagag ccccgcgaac acgcgcaaga tggagttgat ggccgatgcc
     1021 tacggggcct acctcgatgc ccacgccaag ggcttgaact tcacgccgaa gcagatcgac
     1081 gggaccgtcg cggtgtccca tctggtcggc gactgctccg tcgattccct cggccaccac
     1141 ggcacgcccg cccagcgcgc ccgggccgcc cgctggggcg ccgaccttgc actgaagaac
     1201 ggctcgaacg tattgccctc caagcgggtt caggcgctct tcctcacggc gctgcccggg
     1261 atcctgcgcg gctgacccgg tcgcgacccc ggcgcggatg gccgcccacc ccggtggacc
     1321 gccatccgcg ccggcgcgtc tcggccccgc cggggatgaa aggatcggcg gtatgcggtc
     1381 ctattgcgcc atctatgtcg acgtcggcta cctgctcgcg tcgtccgcga cgcgcgtgac
     1441 ggggacctcg ctgcgccgtg gggtcacggt cgatcacgag tcgctcatcc gcggggtcat
     1501 cgagcaggcc gagcaggaca gcggtatgcc gctgcttcgg gtcaactggt acgactcggg
     1561 tggtcgtccg ggcgggatgc cggacatcca ccaggagcat gtcgggttgt tgccgcgggt
     1621 gaagctgcgg ctgggccggc tctcgcagac gggcgagcag aagggcgtcg acctgcgcat
     1681 gggcctggac ctggccacct acggccgcaa ccgggtcgtc gatgtcatct atctgatctc
     1741 cggcgacgac gacctgaccg aggccgtcga agaggcgcag ttccacggcc tgcaggtcgt
     1801 catcatggct gtccccgatc agcgcggccg cccgcacgcc gtcgcgcgca acctccaacg
     1861 cgaggccgac aacgtcatcc tcatcgacgc cgcgacgatc gacgccgccg tcaagccggt
     1921 ggcattgccg ccggcgctca ttcccggcgg ggaggacaag tccgtcgagt ccgcctccgt
     1981 cgctccgcac gaggacgcac ccggaggtgg cctggagccg tcggccgagg ggatccccgc
     2041 gccggccgca tcgcccgaca cgacggaccg gtccgcccaa cctggcgagc cggccgagcc
     2101 ggccgcgccg catccgaacg gcgcccgcgg cacccccgcc gccgtgccgt cccggcccgg
     2161 cccggacacc gggaccgacg cggaccccgg aatcgacgcg gaggccccgg ccccaccgca
     2221 taccgcggca ccgaagccct cggtcctcgc cggcaagcga ccctcgacga tcctgccccc
     2281 gaaggccgcg ccgccgatgt ggacgacgag caccggcggg gcgagccgcg gggaggacat
     2341 cggcctcgtc gacatcgaag ccatcgacgc cgtcgtgcgc caggtcatcg cgacctgggt
     2401 gttgtcggcg acgccggaga tgctcatcga gttgcgcaag agcgaaccgt tcatccccgg
     2461 cgacctcgac cgcaccctgc tgctcgacct gtccagccgc accggcgaat acgacatcaa
     2521 cgacgcctcg cggcatgtca tgcgcgaccg cttctgggag caggtcaagc gcctgcggct
     2581 ggggtaacga aagtcgtcgc gtcgtggcgg cgcggggaca ttccgcgcgc cacctccatg
     2641 cactaagttt cgccgcatga gcagacgggg tcgcacgatc cgccggatcg tgctcggcgt
     2701 cctcgtcctg gccgtggtcg ccgtcatcgg tggctactgg tacgagcggc cactgctgct
     2761 gaccggcacc gggtatgccg cacacaacgc gtgcgcggtg cgtcagatcg ccggtcgcga
     2821 cgacccaacg gcggacctgc cgcccaatcc gctcgtgccc tatctgcgcg attcgaccga
     2881 cggcgcccgc acgacagggt cgctgtgggg gcgcctggcg atccaaaagg cttggtacac
     2941 aaagggatac ggatgcacgc tcgcggatga ggcgcccgat ctcgggacgg cgacggcggt
     3001 cggcgtcaag gacaacccct tctcgggcgc cccgacgccg accccccgca aggccgtggc
     3061 ccaggccctc gccaatgctt tcggcgacga cctgccgcag gatcagcggg ccgctctcgg
     3121 gacccgagcg gtcgtggtcg cccagcacgg caagatcgtc ggggagcggt atgccgacgg
     3181 gttcggtccc tccacgccgc agctgggctg gtcgatgtcg aagagcgtcg ccaacctgct
     3241 cgtcggccgg ctggtcctcg acgaggtcct caccgtcgac gacgcaaaac tgcgcaagga
     3301 ctggaagggc gacaaggcca agatcaccat cgatcagctg atgcggatga gcagcgggct
     3361 gtcgtgggac gagacctacg ctctcggcac gccggtgacg acgatgctct atcgggaaga
     3421 ggacatgggc gcctacgcgg ccggcctcga catggcgcac aagcccggca cctatcagca
     3481 atactccagc ggcagcacca acatcatctg ctcggtcctg cacgagcgca ccgaggtcgg
     3541 cgccgacctg ccccggcagg aactgttcgc caagctcggt ctctcctcag ccgtctggga
     3601 gccggacgcg tcggggacgc cggtatgcag ttcctatctc tgggccacgc cacgtgactg
     3661 gctgacgatc ggccagttcg ccctgcaggg cgggcagtgg gccgaccaac agctgctgcc
     3721 cgaggactgg atggcccagt ccaccgaggt cgtcaaggtc gacaagaccg aagagccagg
     3781 gtatgccgcg agctggtgga ccaacaagga cgccgcgggc aagcgcatct ccgaaatcct
     3841 tccgcgagac gcgtattggg cgcaggggca cgacgggcaa cggctgtatg tcgtgccgtc
     3901 agccgacctc gtcgtcgtcc gcctcgggtt cagcccgcag gtcgagccgg ccgatctgcg
     3961 cgccgacgcg ctcgtcgccg acctggtcgc tgccctaccc gtcaccaaat agccccgcgg
     4021 cctggcaggg tagagggtat gcgcgccatc gaccttgccg agcaggtgcc gaccgtccac
     4081 aaggagacca ccggtgccga ggctgcccgg atcgtcgctg agttccggct caacgggctg
     4141 gtcgttgccg atgattccgg ggtgccgatc gcggtcatcc ccggcagcca gctgctctcg
     4201 gtggtgctgc cgcagtatgt ccgcgacgac ccccacctcg cgcacgccta tgacgaacag
     4261 gctgccgacg acctctgcgc gaagctgacg accgtcaccc ttggcgacct gatcgacgcc
     4321 aaacggctga cgccggcaca gctgccgcag gtcgaacctg acgccacggt ggtcgagatc
     4381 gtctccgcca tgagcgagtt gcactatcca ctcatcgtcg tggccgatca ggctagattc
     4441 cacggagtcg tgacgatgtc ccgcgtgctc gccgccatcg cccaggccgc gggccaggac
     4501 agcgacctga tccgccgccg tctcgacaag gacatcatcg atcgggggcg gtccggctac
     4561 ctcagcgaag ccggcgggga caccgctcgc ggcgcaggtg aggacgcccg gtgacgcttg
     4621 ctgcctggct ggcggtggcg gtcttcgcca gcgcctacat cctgatcgcg accgagaagc
     4681 tcaaccgcgc cgccgtcgcg ctcgggggcg cggcggcgat ggtggccatc ggggcgacgc
     4741 gcggccacgc gatgttcttc tccgaggaga cgggcatcga ctggaacgtg atcttcctgc
     4801 tgttcggcat gatgatcatc gtcggaatcc tcaaggagac aggggttttc gagttcctcg
     4861 cgatctggtc ggcgcagcgc gcccaggcgc ggcccttccg gatgatggtg atgctcatcc
     4921 tcatcacggc cgtcgcgtcg gccctgctcg acaatgtgac gacggtgctg ctcgtcgcgc
     4981 cggtcactct cctgctgtgt gaccggatgg ccctgccgcc ggtgccgttc ctgctcgccg
     5041 aggcgatggc gagcaacatc ggcggcaccg cgacgctcat cggcgatcct ccgaacatca
     5101 tcatcgccag ccgggccgga ttgtccttca acgacttcgt cgtcaatctc gcgccgatcg
     5161 tcgtcgtgct gctggccgtc ttcatcggcc tggcctggct gctgtggggc cggcgtctgg
     5221 cccatgaccc gaagcggtcc tcggcagtca tggcgctgac gccgcgcgag acgatcacca
     5281 atgccgcgtt gttgcgccgg atgtcgctca ttctcgcgct ggtgctgctc ggcttcgtca
     5341 cccacgccgt gacgcatatc gagccctccg tcatcgccct gctgggcgcc ggtgccgcga
     5401 tcctcgtctc ccggctcgag cccgacacct atctcgagga cgtcgagtgg gagacgctgc
     5461 tcttcttcgc gggcctgttc gtcatggtcg gctccctggt ccaccagggc gtgatcgagc
     5521 agctggcgcg cagcgtgacc agcctcatcg acggcaacta tcacgttgcg gcccaaggca
     5581 tcctggtcgg ttcggccgtg ctgtcgggga tcgtcgacaa tatcccctat gtcgcgacga
     5641 tgtcgccatt ggtgcatggt ctcgtcggcc cggccgccgg tctgccaccc gaggaggcct
     5701 cactgtggtg ggcgttggct ctcggcgccg atctcggtgg caatgcgacc gcgatcgggg
     5761 ccagtgccaa tgtcgtcgtc tgtggcatcg cgaaacgcaa cggtcacccg atctcgttct
     5821 gggagttcac gaaatatggc ctgatcgtca cggcagtgac gatcctgtgc acggtcccgt
     5881 atgtgacgct gcgctacttc tagg
//
LOCUS       DAOPJG010000198         5902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14507_length_5902_cov_8.306140, whole genome shotgun sequence.
ACCESSION   DAOPJG010000198 DAOPJG010000000
VERSION     DAOPJG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14507_length_5902_cov_8.306140"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..849
                     /locus_tag="PKU75_14365"
     CDS             133..849
                     /locus_tag="PKU75_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPF82110.1"
                     /translation="MARAKRDGIGTPGGSPERPALFFSGPEEFRAWLTANHETAPELW
                     MGLRKRHVADRGLTWAQAVPEALCFGWIDSVAQRIDADTTRQRWTPRRPGSVWSSVNI
                     ALVEQLIATGRMQPAGLAAFERRRPEKSGVYAYEKPGDQELPPEYAAQLAADPVAARW
                     LELATPTYRRLAIHWVLTAKQQATRDRRMAELVADSARGLLVKPQRYGAVPAWVVRNR
                     ATLGLADPGDGTGASGPEER"
     gene            846..2096
                     /locus_tag="PKU75_14370"
     CDS             846..2096
                     /locus_tag="PKU75_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPF82111.1"
                     /translation="MSAAYGPHDRERWVGEDPALKRTDRDDFARDRGRLIHSAALRRL
                     AAKTQVLAPDSDDFVRNRLTHSLEVAQIGREFGAALGCDADLVEVACLAHDLGHPPFG
                     HNGERALDELCDDIGGFEGNAQTLRILTRLEPKRFHADGRPAGLNLTRASLDSSVKYP
                     WERGDRETPKFGAYADDLPVFDWVRAGRGPGERCLEADVMDWSDDVAYSVHDVEDAVA
                     SGRLDPGMLGDPDEIAGVLSVAHGVYAADLSASLVADAWARLVAAGMVPRSYDGSRRD
                     RAALKDLASRLIGRFVLASEAATREVYGAGPLTRYAARLVVPEETRAEVAVLKAVAAH
                     FVMYAPDRLTIQERQREVIKALVAGFEHDPTRLAADFRADHDQAVDDAARRRVVADQV
                     ASLTDPSAWAFARAWGFGEAAHIH"
     gene            2131..4044
                     /gene="dnaG"
                     /locus_tag="PKU75_14375"
     CDS             2131..4044
                     /gene="dnaG"
                     /locus_tag="PKU75_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPF82112.1"
                     /translation="MAGRIRAEDIAAVKDRTSIVDVVRDHVTLRSAGVGSLKGLCPFH
                     DEKTPSFTVRESAGTFHCFGCGEGGDVVAFVQKIDHLSFVEAVERLASRLGMELQREE
                     GGAGGPEGGLGRRSRLVEAHRIAQEFYTDLLITSPDARAARDFLRQRDFNGAQCEPFG
                     VGFSPRGGEDLARLLRGKGFSDEEMTLAGLVGRGARGIYDRFRGRLMWPIRDTSGDTI
                     GFGARRIFDDDRIEAKYLNTTETTIYKKSQVLYGIDLAKRVISQGRQAVLVEGYTDVM
                     AAHLSGVQSAVATCGTAFGVDHIKLLRRLMRDEPGLDPARIVFTFDGDTAGQKAAMKA
                     FAEDQRWTAQSFVAVAADGMDPCDLRIRKGADAVKTLVDDAVPMFEFAVRTLIARCDL
                     GTAEGRVQAMKVVAPVINSVKDPSLRPEYQRTVAGWIGVGVEQLADQMRRAGTLKAPE
                     DGPHRRDADAPEPSAAELAAAYPMPDLRDPLVNAEAQLLQVVLQYPNAVPPDLYDTVP
                     PDAFVAPAHRAVFDAMRSVVVSEGMTLAQWVDGVTQQAPLAAQPLVSALSVATLPTSI
                     DPATGGPDQRYVDSLVARVREVSLTRQIADAMSWMRRLSSDPDADPGQVRQVGTQLQD
                     LQRQLAILREGVN"
     gene            4046..4564
                     /locus_tag="PKU75_14380"
     CDS             4046..4564
                     /locus_tag="PKU75_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82113.1"
                     /translation="MKLLRRKDDTPSLPSEVRARLGLAPGERPLAAARDEGGTWVVVG
                     TAHLYAVPAAGEILVRPWHLVDHGSWDHDNFTLTVTWVDGARPQQWVFRDTMQVLAAF
                     RERVQASVVLVEEVDVGVSGSGRVVIRKNLGDQSLLDQTILGRRVRLEDPGVREAFAD
                     ARARLREQVGLD"
     gene            complement(5282..5354)
                     /locus_tag="PKU75_14385"
     tRNA            complement(5282..5354)
                     /locus_tag="PKU75_14385"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5319..5321),aa:Asn,seq:gtt)
     gene            complement(5540..>5902)
                     /locus_tag="PKU75_14390"
     CDS             complement(5540..>5902)
                     /locus_tag="PKU75_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPF82114.1"
                     /translation="PTSTPAPAPKPTPEPKVATTPDYTKSVPAPEAEAAPAPPKAAPA
                     PRADKPKPDHDRDRDRADRSDRNDDDDYRVYTIKPGDTLSEIGERFGVPYMKIAKLNN
                     ISNPDRIYAGNKIKIPNG"
BASE COUNT          835 a         2019 c         2156 g          892 t
ORIGIN      
        1 caccacgcgc cggtggccgg gtcacctccc caacggtgcc ggatagggcc ggatcccgcg
       61 gatcgggagg cgtggttccg cgacatgccg cgctgaggtg gcctgtcggt cacctctggc
      121 aaggtggtgg ccatggcgag ggcgaagcgc gatggcatcg ggaccccggg aggcagtccc
      181 gagcggcccg cgctgttctt ctccggtcct gaggagttcc gggcgtggct gaccgccaac
      241 cacgagacgg cgccggaact gtggatgggc cttcgcaagc gccatgtcgc cgaccggggg
      301 ctgacctggg cccaggccgt gcccgaggcg ctgtgtttcg gctggatcga ctcggtcgcg
      361 caacggatcg acgcggacac cacccggcag cgctggacgc cccgtcgccc gggcagcgtg
      421 tggagttcgg tgaatatcgc cctcgtggag cagttgatcg ccacgggccg gatgcagccg
      481 gccggcctgg ccgccttcga gcgccgaagg cccgagaagt ccggcgtcta cgcctacgag
      541 aagccgggcg atcaggagtt gccgccggag tatgccgcgc agctggccgc cgatccggtg
      601 gccgcccgct ggctcgagtt ggccaccccc acctatcgcc gcctggcgat ccattgggtg
      661 ctcacggcga agcagcaggc tacccgcgat aggcgcatgg ccgagttggt cgctgacagc
      721 gcccgcgggc tgctggtcaa accgcagcgg tacggcgcgg tgccggcctg ggtggtgcgc
      781 aaccgggcca cgctcggcct tgccgacccc ggcgacggca ccggcgcgag cgggccggag
      841 gagcggtgag cgcggcatac ggcccgcacg accgggagcg gtgggtcggc gaggacccgg
      901 cgctcaagcg caccgaccgc gacgacttcg cccgcgaccg cggacggctc atccactcgg
      961 cggcgctgcg gcggctcgcg gccaagacgc aggtcctcgc gcccgacagc gacgacttcg
     1021 tgcgcaaccg gctcacccac tcgctcgagg tggcgcagat cgggcgcgag ttcggggccg
     1081 cgctcggctg cgatgccgac ctggtcgagg tggcctgcct ggcgcacgac ttgggtcacc
     1141 cgccgttcgg gcacaacggc gaacgcgctc tcgatgagtt gtgtgacgac atcggtggtt
     1201 tcgagggcaa cgcgcagacg ctgcgcatcc tgacgcgctt ggagcccaag cgttttcacg
     1261 ctgacggccg tcctgcgggc ctgaacctca cgcgcgcgag cctggactcc agcgtgaagt
     1321 atccgtggga gcgcggcgat cgcgagacac ccaagttcgg cgcgtatgcc gatgacctgc
     1381 cggtgttcga ttgggtgcgg gcggggcgcg gaccggggga gcggtgcctg gaggccgacg
     1441 tcatggactg gtccgacgac gtggcctatt cggtgcacga cgtcgaggat gcggtggcca
     1501 gcggccggct cgatccgggg atgctgggcg accccgacga gatcgccggc gtgctctccg
     1561 tcgcgcacgg ggtgtatgcc gcggacctga gcgcgagcct cgtcgccgac gcctgggcgc
     1621 ggctcgttgc cgccgggatg gtgccgcgtt cctatgacgg cagccgacgc gaccgggcgg
     1681 cgctgaaaga cctggcgtcg cggctgatcg gccggttcgt cctggcctcc gaagcggcaa
     1741 cgcgcgaggt ctacggtgcg ggcccgctga cccggtatgc cgcccggctc gtcgtcccgg
     1801 aggagacccg ggccgaggtg gccgtcctga aggccgtcgc cgcgcacttc gtcatgtacg
     1861 ccccggaccg gctcacgatc caggagcggc agcgcgaggt gatcaaggcc ctcgtggccg
     1921 gcttcgagca cgacccgacc cgcctcgccg ccgacttccg cgccgaccat gaccaggctg
     1981 tggacgacgc cgcgcggagg cgcgtcgtcg ctgaccaggt ggccagcctg accgacccta
     2041 gcgcctgggc tttcgcccgc gcgtggggat tcggcgaggc ggcccacata cactgacgtc
     2101 gcacctgcgg gcagcgacgg aaggagcggt gtggccgggc ggatccgagc cgaggacatc
     2161 gcggccgtca aggaccgcac ctccatcgtc gacgtcgtgc gcgaccacgt gactctgcgc
     2221 agcgccggag tcggctcgct caaggggttg tgtcccttcc acgacgagaa gaccccctcg
     2281 ttcacggtcc gcgagagtgc cggcaccttc cactgtttcg gctgcggcga gggcggcgac
     2341 gtcgtcgcct tcgtccagaa gatcgaccac ctcagcttcg tcgaagcggt cgagcgcctc
     2401 gcctcccggc tcggcatgga actgcagcgg gaggagggcg gggcgggtgg tccggagggc
     2461 ggcctcgggc ggcgctcccg gctggtcgag gcgcaccgga tcgcgcagga gttctatacc
     2521 gacctgttga tcacgagtcc cgacgcccgg gcagcgcggg acttcctgcg ccagcgcgac
     2581 ttcaacggcg cgcagtgcga gccgttcggg gtcgggttct cgccgcgggg tggggaggat
     2641 ttggcacggt tgttgcgggg caagggtttc agcgacgagg agatgacgct ggccggcctc
     2701 gtcgggcgtg gcgcgcgcgg catctacgac cgcttccgcg gccggctgat gtggcccatc
     2761 cgcgacacct ccggcgacac catcggcttc ggcgcgcgcc ggatcttcga cgacgatcgg
     2821 atcgaggcga agtacctcaa cacgaccgag acgacgatct acaagaagag ccaggtgctc
     2881 tatggcatcg acctcgccaa gcgggtcatt tcccaagggc ggcaggccgt gctggtcgag
     2941 ggctataccg acgtcatggc cgcccacctg tccggtgtcc agtcggccgt cgccacctgc
     3001 ggcacggcct tcggggtcga ccacatcaag ctgctgcgcc ggctgatgcg tgacgagccc
     3061 ggcctcgacc cggcccggat cgtcttcacc ttcgacggcg acaccgccgg gcagaaggcg
     3121 gcgatgaagg ccttcgccga ggaccagcgc tggacagcgc agtccttcgt cgcggtcgcc
     3181 gccgacggga tggacccgtg cgatctgcgc atccggaagg gcgccgacgc cgtcaaaacc
     3241 ctcgtcgacg acgccgtccc gatgttcgag ttcgcggtgc ggacgctgat cgcgcgctgc
     3301 gatctcggca ccgccgaggg ccgggtgcag gcgatgaaag tcgttgcccc ggtgatcaat
     3361 tcggtcaagg accccagttt gcgacccgag taccagcgca cggtcgcggg gtggatcggg
     3421 gttggcgtcg agcagctggc cgaccagatg cggcgcgccg ggaccctcaa ggcgcccgag
     3481 gacgggccgc accgtcgcga cgccgacgct ccggaaccca gcgcggccga gctcgccgcg
     3541 gcatacccca tgcccgacct gcgcgacccc ctcgtcaacg ccgaagccca actcctgcag
     3601 gtggttctgc agtatcccaa cgccgtcccg cccgacctct acgacacggt gccgcccgat
     3661 gcgttcgtcg cgccggcaca ccgggcggtg ttcgacgcga tgcgcagtgt cgttgtctcc
     3721 gagggcatga cgctggcgca gtgggtggac ggcgtgaccc aacaggctcc gctggcggcg
     3781 cagccactgg tctcggcgct gtcggtcgcg accttgccga cgtccatcga tcccgcgacg
     3841 ggcggccccg accagcggta tgtcgattcg ctcgtcgccc gcgtccgcga ggtctccctg
     3901 acccggcaga tcgccgacgc catgtcgtgg atgcgccggc tctcgtccga cccggacgcc
     3961 gaccccggcc aggtgcgcca ggtcggcacc caattgcagg atctgcagcg ccagctggcc
     4021 attcttcgcg aaggagtgaa ctgacgtgaa actgttgcgc cgcaaggacg atacgccctc
     4081 gctgccgtcg gaggtcaggg cccgcctcgg cctcgctccg ggcgaacgcc ccctcgccgc
     4141 ggcccgcgac gaaggtggca cgtgggtggt ggtcgggacc gcgcacctgt atgccgtgcc
     4201 agccgcgggc gagatcctcg tgcggccgtg gcacctggtc gaccacggct cgtgggatca
     4261 cgacaacttc accctcaccg tcacgtgggt ggacggggcc cgcccacagc agtgggtctt
     4321 ccgggacacg atgcaggtgc tggccgcgtt tcgggagcgg gtccaggcct cggtcgtcct
     4381 ggtcgaggag gtggacgtgg gcgtcagcgg ctcgggccgg gtcgtcatcc gcaagaacct
     4441 cggcgatcag tcgttgctgg accagacgat cctcggccgg cgcgtgcggc tggaggatcc
     4501 gggtgtgcgc gaggcattcg ccgacgcccg cgcccgcctt cgcgaacagg tcggtctgga
     4561 ctagccggcg gtttcgcgac ccggggacag gttctcgttg tggggccggc ccagctagag
     4621 gccggcccca caccgagatc ttggtccgag tcgacgaaca agcgggcccc tcaacgaggg
     4681 cctgcccgct gcgctccgct cggtcaggcg ttgctgacgc ggctgcccgg agcgccgtgg
     4741 tgcccggccg gggcgtcggc ctggccgttc gccggggccg ggcgttcgcc gccgccacct
     4801 cggggtccgc cggggcagtc ggtgtcgtcg gtgcccggac gcgggtttcc tttcgaaagc
     4861 aggcgccact gagacaccgg tccgagggaa acgggtcggt gtgttctcgc ggttggtgaa
     4921 ccaccggcgt cggggccggt gctcagtcat cccaggggcg gacggtccgc ccggcggccg
     4981 gcggcggatg aggagttgcg ctcacgccgg ccgatgcggt gtgcatttgt cgcgggttca
     5041 gccccgctga caacgggaac ggctgcaacc cggttcggcc gaagcattgg tggactccaa
     5101 cgcctcaccc gtgctcttct ggcagcccgg caccgcgtcg gactacgcga tcacactcag
     5161 cgaactcagc tcccaagccg acgcgatgga cgccatttcg gcggactacg cgcgctgggt
     5221 caaccgggcg atgaactggg agaaggggcg cgcggcattc ggcgcggcgg ttgaagaaaa
     5281 gctcccccgg ttggactcga accaacaacc cttcggttaa cagccgaatg ctctgccagt
     5341 tgagctacag gggatcgtct ggcacgccct cgcaggcgct cggaaaccat agcaaagagt
     5401 gctcggcaat ccgaaaccga aacagccgag acgtcacgag cggggtggaa ggcccccttc
     5461 caccccgctc gctcaccgac ctctccaccg ccccgggcgc cgtatgtggt ggcagacccg
     5521 tgggacgggg accaagcggt cagccgttcg ggattttgat cttgttgcct gcgtagatcc
     5581 gatccgggtt ggagatgttg ttcagcttgg cgatcttcat gtacgggacg ccgaaccgct
     5641 cgccgatctc ggacagggtg tcgccaggct tgatcgtgta aacgcggtaa tcgtcgtcat
     5701 cgttccggtc ggagcggtcc gcgcggtcgc ggtcccggtc gtggtccggc ttgggcttgt
     5761 ccgcgcgcgg agcaggggca gccttcggcg gcgccggggc ggcctcggcc tcgggagccg
     5821 ggacggactt ggtgtagtcg ggcgtcgtgg cgaccttggg ctcgggcgtc ggcttggggg
     5881 ccggcgccgg ggtggaggtt gg
//
LOCUS       DAOPJG010000199         5899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14521_length_5899_cov_7.698665, whole genome shotgun sequence.
ACCESSION   DAOPJG010000199 DAOPJG010000000
VERSION     DAOPJG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14521_length_5899_cov_7.698665"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKU75_14395"
     CDS             <1..197
                     /locus_tag="PKU75_14395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851607.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPF82115.1"
                     /translation="LSQNTYVAKQFTGIEGSTVPLNETIEAFTKICDGEYDHVGEQAF
                     FMCGGLDDVERQWAEISKNL"
     gene            410..673
                     /locus_tag="PKU75_14400"
     CDS             410..673
                     /locus_tag="PKU75_14400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPF82116.1"
                     /translation="MAPLQVELVAADREVWSGEASMVSARSVEGDMGIMPGHEPTLVV
                     LAEGEVRIQGTDGPRKAHIDGGFLSVDQDKVTIVSEVVEGIDG"
     gene            707..1147
                     /locus_tag="PKU75_14405"
     CDS             707..1147
                     /locus_tag="PKU75_14405"
                     /inference="COORDINATES: protein motif:HMM:NF022199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 family protein"
                     /protein_id="HPF82117.1"
                     /translation="MGLLEITELVLGVLLALLLVCIGFVLVRRHLIADGKPLMLCALR
                     TPAKPEWRLGLLRFGGQSLDWFTIFGPALRPATTWNRSTFQLGPPAPSAEPVPVLGEA
                     LTAPASDQGGDFEIAMQPAALTAMRAWMESSPPGSSTSLPGQFT"
     gene            complement(1135..1821)
                     /locus_tag="PKU75_14410"
     CDS             complement(1135..1821)
                     /locus_tag="PKU75_14410"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPF82118.1"
                     /translation="MVNLTRIYTRTGDDGSTALGDFTRTRKTDARLAAYADTNEANAA
                     IGVAIAAGQLRDDVREVLLRVQNDLFDVGADLATPVRPAYDHEPLRVQAEWVDELEAD
                     CDHFNGRLESLRSFILPGGTAASAYLHLACTIVRRAERATWAAIERYDVTPAPAPAPG
                     GINPLTATYLNRLSDLLFILARVANVDDGGDVLWRPGGGRAAAPVKRTRTIAADDPLT
                     DDDAPRDQVN"
     gene            1986..2297
                     /locus_tag="PKU75_14415"
     CDS             1986..2297
                     /locus_tag="PKU75_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPF82119.1"
                     /translation="MVVADHAEGRLVTITGVIDVHSAADLRGDLHALIDDGRGTPLLL
                     DLGRAEIADATGLGLLLECHRRGRRHGRQMRLVAVSDGSERLFRRLALRRQFAPRPAY
                     R"
     gene            2400..4421
                     /locus_tag="PKU75_14420"
     CDS             2400..4421
                     /locus_tag="PKU75_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HPF82120.1"
                     /translation="MTDRDRPWVMRTYAGHSSAAASNELYRRNLAKGQTGLSVAFDLP
                     TQTGYDPDHALARGEVGKVGVPISHIGDMRALFDGIPLTAMNTSMTINATAMWLLALY
                     QVVAEEQAIAAGEDPDAARHTLAGTTQNDIIKEYLSRGTYVFPPGPSMRLLTDVVTYT
                     VREIPKWNPTNVCSYHLQEAGATPVQEIAFAMSTAIAVLDEVRDSGAVPAEEFGDVVA
                     RISFFVNAGVRFVEEMCKMRALVALWDELTQERYGVTDAKQRRFRYGVQVNSLGLTEA
                     QPENNVQRIVLEMLAVTLSKDARARAVQLPAWNEALGLPRPWDQQWSLRIQQVLAYES
                     DLLEYDDLFAGSPVVEAKVAELVAGAKEEMARIAEMGGAVAAVESGYMKSALVASHAR
                     RRGRIEAGEDIIVGVNKFETTEPNPLTADLTTAIQTVDHDVEVKAAEAVQAWRAARDA
                     DPAKKERAAAALARLKEDAAGTGSLMPATLECARAEVTTGEWAGALREVFGEFRAPTG
                     VSGSVGVAEAGDELMAVRASVADLEKELGEKLRVLVGKPGLDGHSNGAEQIAVRARDA
                     GFEVIYQGIRLTPEQIVAAAVAEDVHLVGVSILSGSHMELVPEILSGLRDAGAGDIPV
                     VVGGIIPESDAVKLTDQGVAAVFTPKDFGLNEIMARFVEIIRASTSVPA"
     gene            4522..5127
                     /locus_tag="PKU75_14425"
     CDS             4522..5127
                     /locus_tag="PKU75_14425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPF82121.1"
                     /translation="MSLHCPATLIVARHAEAEYSHGDLLSDEGGRLTAHGREQAAQLA
                     TRLGEKRIALVYTSPLARAVETGRIAATGLGVGHREMAGLQEFSVGDLVGEPVDDERL
                     TTTYAAWLAGELSHRIPGAESGEEVVTRFREALSAIADLHRGEHVLVISHGGVMSLGL
                     PNVATGGPAGSPPPLANCASVELSAGDDGWTLGPWAPAEAT"
     gene            complement(5124..5819)
                     /gene="nucS"
                     /locus_tag="PKU75_14430"
     CDS             complement(5124..5819)
                     /gene="nucS"
                     /locus_tag="PKU75_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HPF82122.1"
                     /translation="MRVVIAKCSVDYEGRLTAHLPLATRLLMVKADGSVLVHSDGGSY
                     KPLNWMSPPCSMAEAVPDEDEAADGVTLVWVVRHAKSDDALRVRIHEVVSDATYDLGV
                     DPGLVKDGVEAHLQALLAEHIHTLGDGYSLVRREFMTAIGPVDILCRDGDGVTVAVEI
                     KRRGEIDGVEQLTRYLELMNRDPHLAPVTGIFAAQQIKPQARTLAEDRGIRCVVLDYD
                     ELKGIDTSEHRLF"
BASE COUNT          897 a         2023 c         2064 g          915 t
ORIGIN      
        1 tcctgtcgca gaacacctac gtcgccaagc agttcaccgg catcgagggc tcgacagtcc
       61 cgctgaacga gaccatcgag gccttcacga agatctgcga cggcgagtac gaccacgtcg
      121 gcgagcaggc ctttttcatg tgcggtggcc tcgacgacgt cgagcgccag tgggcggaga
      181 tttccaagaa cctctgattt cgctcctcat caccgcagca gggatccccc cggacgtcgt
      241 ccggggggat ccctgcttcg gcgatcgtcg aggctcggcg atcgccgaag gaatcgcgat
      301 caacgcggca tcccggccga cccccgcggg cgaagagcac gctgggcggt cggttagact
      361 tggtggcaat ccttctgtcg aaacccgttg atgccgaaag gttgctctcg tggctccgtt
      421 gcaggttgaa ctggtcgccg ccgaccgcga ggtctggtcc ggcgaggcga gcatggtctc
      481 ggcgcgctcc gtcgagggcg acatgggcat catgcccggg cacgagccga cgctggttgt
      541 gctcgccgag ggcgaggtcc gtatccaggg caccgacggc ccgcgcaagg cccacatcga
      601 cggcggcttc ctgtcggtcg accaggacaa ggtcaccatc gtctccgagg tcgtcgaggg
      661 catcgacggc tgacgccgcg atcgcctcgc agccggagga gggcgcgtgg gactgctcga
      721 gatcaccgag ctggtcctcg gagtgctgct cgcgctcctg ctggtctgta tcggcttcgt
      781 cctggtgcgc cgacacctca tcgcggacgg caagcccctg atgctgtgcg cgctgcgaac
      841 cccagccaag cccgagtggc ggctggggtt gctgcgtttt gggggccaga gcctggactg
      901 gttcaccatc ttcggtcctg cgctgcggcc ggcgaccacg tggaaccgct cgaccttcca
      961 gctcggccct cccgcaccga gcgccgagcc cgtccccgtg ctgggggaag ccctgacggc
     1021 cccggcctcc gatcagggcg gggacttcga gatcgcgatg caaccggccg cgctgaccgc
     1081 catgcgggcc tggatggagt cctcgccacc tggctccagc acgagcctgc cgggtcagtt
     1141 cacctgatcc cggggcgcgt cgtcgtcggt gagcgggtca tccgcagcga tcgtcctcgt
     1201 ccgcttgacc ggtgccgccg cccgcccgcc accgggtcgc cacagcacgt cgcccccgtc
     1261 gtccacattg gcgacccgcg cgaggatgaa caacaggtcc gagagccggt tgagataggt
     1321 cgcggtgagc gggttgatcc cacccggtgc cggcgcgggg gcgggggtca cgtcataccg
     1381 ctcgatcgcc gcccaggtgg cgcgctcggc ccgccgcacg atggtgcacg cgaggtgcag
     1441 gtacgccgag gcggccgtcc cgccgggcag gatgaaggag cgcagcgact cgagccggcc
     1501 gttgaagtgg tcgcaatccg cctccaactc atcgacccat tccgcctgca cccgcagcgg
     1561 ctcatggtcg tatgccggcc gcaccggcgt cgcgagatcc gcaccgacgt cgaagaggtc
     1621 gttctgcacc cgcagcagca cctcgcgcac gtcgtcgcgc agctgcccgg cggcgatcgc
     1681 caccccgatc gccgcgttgg cctcgttggt gtcggcgtag gcagccaggc gggcgtcggt
     1741 cttgcgcgtg cgggtgaagt cgccgagcgc ggtgctgccg tcgtctccgg tgcgggtgta
     1801 gatgcgggtg aggttgacca tgggtccagc ctgccgcatg cgcaacgtca tcacattacg
     1861 gtcacgagat ccgggagtga cgcgtgtcac ccaaccggat ggggccctac tgcgtatata
     1921 acagggtgaa ccggatgcag ggggttggag ttatgggacc ggcgctacgc gagaggaccg
     1981 cccaggtggt ggtcgccgac cacgccgagg gacgcctcgt gacgatcacg ggcgtcatcg
     2041 atgtccactc ggcggccgac ctgcgaggcg atctgcatgc gttgatcgat gacggccggg
     2101 ggaccccgct gctgctcgat ctcggccggg ccgagatcgc cgacgccacc gggctcgggt
     2161 tgctcctcga atgccaccgc cggggccggc gccacgggcg gcagatgcga ctggtcgccg
     2221 tcagcgacgg ttcggagcgg ctgttccgcc ggctggccct gcgccggcag ttcgcgccgc
     2281 gacccgcata ccgctgatcc acacccactg cagcgtcatg ccgggaccgc ccggtctcac
     2341 cggtgtcatt ggtcacgagc ccggcttcga ggcgccggcc gccgggtagc gttcttggca
     2401 tgactgatcg cgatcgtccc tgggtgatgc gcacctatgc cgggcactcg agcgctgcgg
     2461 cgagcaacga gctctaccgg cgcaacctgg ccaagggtca gacgggcctg tccgtcgctt
     2521 tcgacctgcc gacgcaaacc ggttacgacc ccgaccacgc cctcgcccgc ggcgaggtcg
     2581 gcaaggtcgg cgtcccgatc agccacatcg gcgacatgcg cgcgctcttc gacggcatcc
     2641 cgctgacggc gatgaacacc tcgatgacga tcaacgccac cgcgatgtgg ttgctggcgc
     2701 tctaccaagt cgtcgcggag gagcaggcga tcgcggcggg cgaggacccg gacgcggcgc
     2761 ggcataccct cgccggcacc acccagaacg acatcatcaa ggaatacctc tcccgcggca
     2821 cctacgtctt cccgcccggc ccgtcgatgc gcctgctcac cgacgtcgtg acctacacgg
     2881 tgcgggagat cccgaagtgg aacccgacca atgtgtgcag ctatcacctg caggaggccg
     2941 gcgcgacccc agtgcaggag atcgccttcg cgatgtccac ggcgatcgcc gtgctcgacg
     3001 aggtccgcga ctccggggcc gtgccggcgg aggagttcgg cgatgtcgtc gcccgcatct
     3061 ccttcttcgt caacgccggg gtgcgcttcg tcgaggagat gtgcaagatg cgcgcgctgg
     3121 tcgcgttgtg ggacgagctg acccaggagc ggtatggcgt caccgacgcc aagcagcgtc
     3181 gcttccgcta cggggtgcag gtcaactccc tcggcctgac cgaggcgcag ccggagaaca
     3241 acgtccagcg catcgtgctc gagatgctgg ccgtgacctt gtccaaggac gcgcgggcgc
     3301 gcgcggtgca actgccggcg tggaacgagg cgctcggcct gccgcgcccg tgggaccagc
     3361 agtggtcgct gcggatccag caggtgctcg cctacgagtc ggacctgctg gagtatgacg
     3421 acctgttcgc cgggtcgccg gtggtcgagg cgaaggtcgc cgaactcgtc gccggcgcca
     3481 aggaggagat ggcgcggatc gccgagatgg gcggggcggt cgcagccgtc gagagcggct
     3541 atatgaagtc ggccctcgtt gcctcgcatg cccgtcgccg ggggcgtatc gaggcgggtg
     3601 aggacatcat cgtcggtgtc aacaagttcg agaccaccga gcccaacccg ctgacggccg
     3661 acctgacgac cgccatccag accgtcgacc acgacgtcga ggtcaaagcg gccgaagccg
     3721 tgcaggcctg gcgcgcggcg cgcgacgccg accccgccaa gaaggagcga gccgcggccg
     3781 cgctggcccg gctcaaggag gatgccgcgg gcaccggcag cctgatgccc gcgaccctgg
     3841 agtgcgcccg ggccgaggtg accacgggcg agtgggccgg ggcgctgcgt gaggtattcg
     3901 gcgaattccg ggctcccact ggggtttccg ggtccgtcgg cgtggccgag gccggcgacg
     3961 agctgatggc cgtgcgggcg tcggtggccg acctcgagaa ggaactcggc gagaagctgc
     4021 gcgtcctcgt cggcaagccc ggcctcgacg gccacagcaa tggcgccgag cagatcgccg
     4081 tccgggcccg ggacgccggc ttcgaggtga tctatcaggg cattcgcctc acgcccgagc
     4141 agatcgtcgc cgcggctgtc gccgaggacg tccacctcgt cggcgtctcc atcctgtccg
     4201 gctcccatat ggagctcgtg cccgagatct tgagtggcct gcgggacgcg ggggcgggcg
     4261 acatccccgt cgtcgtcggt ggcatcatcc ccgagtccga cgccgtcaaa ctgaccgacc
     4321 agggcgtcgc cgccgtcttc acccccaagg acttcggtct caacgagatc atggcccgtt
     4381 tcgtcgagat catccgggcg tcgacctcgg tccccgcctg acccgaggaa tctctgctag
     4441 caaaggctgg tcgaatcagc acacctagca cggtgtgctg atttgacgac tctctgctga
     4501 gttgcggcta ggtttggccg ggtgagtttg cactgcccgg cgacgctgat cgtcgcgcgc
     4561 cacgcggagg ccgagtattc ccatggggat ctgctctccg acgagggtgg gcgcctgacg
     4621 gcgcacggcc gggagcaggc ggcgcaactg gcaacacggc tgggcgagaa gcggatcgcg
     4681 ctcgtctaca cgagtcccct cgcgcgggcc gtcgagacgg gccggatcgc cgcgacaggc
     4741 ctcggtgtcg ggcaccgtga gatggccggc ttgcaggagt tcagcgtcgg ggacctggtc
     4801 ggcgaacccg tcgacgacga gcgcctgacc acgacgtatg ccgcgtggct ggcgggggag
     4861 ctgagccacc gcatacccgg ggccgagtcg ggcgaggagg tcgtgacccg gttccgtgag
     4921 gccctctcgg cgatcgccga cctgcaccgc ggcgagcacg tgcttgtcat ctcgcacggc
     4981 ggcgtgatgt cgctcgggct cccgaacgtg gccacgggtg gtcccgccgg cagcccaccc
     5041 ccgctcgcga actgcgcgag cgtggaactg agcgccggcg acgacggctg gaccctcggc
     5101 ccgtgggcac ccgcggaggc gacttagaac aaccggtgct cggaggtgtc gatgcccttg
     5161 agttcgtcgt agtcgagcac cacgcatcgt atgccgcggt cctcggccag ggtgcgcgcc
     5221 tgcggcttga tctgttgtgc cgcaaagatg cccgtgaccg gggcgaggtg cgggtcacgg
     5281 ttcatcagtt ccaggtagcg ggtcagctgc tcgacgccgt cgatctcgcc gcggcgcttg
     5341 atctccacgg caaccgtcac cccgtcaccg tcgcggcaga ggatgtcgac cggtccgatg
     5401 gcggtcatga attcgcgccg cacgagcgag tagccgtcgc cgagggtgtg gatgtgttcg
     5461 gcgagcaagg cctgcaggtg ggcctcgacg ccatccttga ccaggcccgg gtcgacgccg
     5521 aggtcgtagg tcgcatccga gaccacctcg tggatccgca cgcgcaaggc atcgtcggac
     5581 ttcgcgtgcc gcacgaccca caccagggtc accccgtcgg ccgcctcgtc ctcgtcgggc
     5641 acggcctcgg ccatcgagca cggcggcgac atccagttca gcggcttgta ggacccgccg
     5701 tcactgtgca cgagcaccga gccgtcggct tttaccatga gcagccgggt ggccagcggg
     5761 aggtgggccg tgagccggcc ctcgtagtcc acactgcact tggcgatcac gacgcgcacg
     5821 aggcagaact ctacgtcgcc cgcccgcaca aagtccgacg tttggcgagt caggacccgc
     5881 caaacgtcgg acttagcgg
//
LOCUS       DAOPJG010000200         5896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14530_length_5896_cov_6.722821, whole genome shotgun sequence.
ACCESSION   DAOPJG010000200 DAOPJG010000000
VERSION     DAOPJG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14530_length_5896_cov_6.722821"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..805
                     /locus_tag="PKU75_14435"
     CDS             164..805
                     /locus_tag="PKU75_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF82123.1"
                     /translation="MVRDRSPRTATARGKLLAAAFDLIRAQGLAATSVDQLCERAGVT
                     KGAFFHHFATKEALAVAAAQEWGAMTGAFFAADGYHQQRDPLERIFAYLDLRASLIEG
                     GPAAYSCVAGTMVQEAFATSPAIRDACGTMFFEHAATLESDIQAAIDQHAPALEVTAR
                     SLAVHILVVLHGAFVVSKAGDDPQIVLDSIEHLRRYLRQLFTAEANHPKEKES"
     gene            802..1296
                     /locus_tag="PKU75_14440"
     CDS             802..1296
                     /locus_tag="PKU75_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF82124.1"
                     /translation="MSTTTNAARYQTCLWFDADGLEAAEHDTSLLPDAEITGVSRYPD
                     DVDKGTPGAVMQVDFRLGDQRYVALNGGPQFPHSETVSLQVYVETQEDLDRIWAGLLA
                     GGGEETMCGWLRDRFGLSWQVIPTRLGELLADPDPERVGRVVRQLQAMTKLDIAPLEA
                     AYNG"
     gene            complement(1360..1707)
                     /locus_tag="PKU75_14445"
     CDS             complement(1360..1707)
                     /locus_tag="PKU75_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HPF82125.1"
                     /translation="MTTPAPRPPRDYPFEAPWQAQVFAMTVALQDQGLVGAEEWAQAL
                     GARIAASAVPDGSDYYECWAAALGDLLAAKELLSREDLLRTTAAWHAAAARTPHGHPI
                     VLDAGGSRAGGVT"
     gene            complement(1704..2342)
                     /gene="nthB"
                     /locus_tag="PKU75_14450"
     CDS             complement(1704..2342)
                     /gene="nthB"
                     /locus_tag="PKU75_14450"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HPF82126.1"
                     /translation="MNGVHDVGGMQCFGAVRPEPDEPVFHAEWEGRVLALTLATGALG
                     AWNIDQSRSARESLPPAVYLSSSYYRIWFEAPEGLLGRSGLLDGGFAARARTWPEMKS
                     ALDASNSFARETDSPPQFAVGDRVRARMIHPAGHTRLPRFVRGQVGTIVLVHGSQVFP
                     DRDAVPIGEPADQTPEWIYAVEFSGTDLWGPDSDPTVTVCVDAWEPYLEAAS"
     gene            complement(2339..2971)
                     /gene="nthA"
                     /locus_tag="PKU75_14455"
     CDS             complement(2339..2971)
                     /gene="nthA"
                     /locus_tag="PKU75_14455"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HPF82127.1"
                     /translation="MADDHDHDHSGGSELSEMDARVRALETLLTERGLVDPAALDGIV
                     ETYEHEIGPHRGAQVVARAWTDPDFREWLVRDATTAVASMGYGGRAGEHLIALANTPE
                     VHNMVVCTLCSCYPWPVLGLPPVWYKTPAYRAKAVRDPRGVLADFGVTLPDSTRIRVW
                     DSTSEQRYLVVPMRPDDTEGWDAERLATLVTRDSMIGTGLASAPSQETVS"
     gene            complement(3024..3497)
                     /locus_tag="PKU75_14460"
     CDS             complement(3024..3497)
                     /locus_tag="PKU75_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPF82128.1"
                     /translation="MKITDNGPEPNVFDIETATKENTTYRTVAWTGKYLQVTLMSIEP
                     GASIGLEAHPETDQFLRLDAGEGVCKMGPAKDDLAETEVSDGWSIQVPAGTWHDVVNT
                     GSEPLRLYAVYAPSHHAQGKVHETAEDAEADEKAGTDEPPEWTVQPGEGAADKHA"
     gene            complement(3654..4616)
                     /locus_tag="PKU75_14465"
     CDS             complement(3654..4616)
                     /locus_tag="PKU75_14465"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF82129.1"
                     /translation="MSNDRHDYESHEDRDDFGGERRHRGPRGPHEHRGHHNGPRGRRP
                     QGDSPWEQIFGADGPFGPRGPFGKGGLFGPQGADMARAIWENATAGAAHPQDASAPPR
                     DWERGPRGRRDWAEDGEFGRPDGDRGKRRGGPDGPRGEGRGRRGHGGPGGRRGRRPKG
                     DVRLAALLLIAEEPRNGYQIIEELASRSSGAWKPSSGAVYPALAQLEDEGLIEAFDND
                     GRKAFRLTDAGQQAVEDNEAPAPWETATADASEAMGGRPGGEMWQAFGQVAMAAKAVS
                     ATRDAATMTEAAKVLEDTRRALYRILADGPQDEPVDAEIVDDES"
     gene            4809..>5896
                     /locus_tag="PKU75_14470"
     CDS             4809..>5896
                     /locus_tag="PKU75_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HPF82130.1"
                     /translation="MRPKPSGIGETFHELSLRAFDLSERAGRAGLDSNRRRVQRWREH
                     LFMMSQIAIAAGLAWFLGQHLLGHQLPFFAAVAAIICLGLSFGQRISRVVQVAVGVFV
                     GVFVGDLFVALVGTGAWQISLVVFVAMSIAIWVGAKILMVNQAGIQAATVVTLFPNPD
                     EGVSRWLDALLGCAIALVFALVAPTSPVQKPRMKAAQVLYEAAETLYAATAALRSGDE
                     NAADEVLARARSTEGKLRALSEASSEGIALVRYSPLMRQHRDHVQAVAEIVAPLDRML
                     RNLRVLARRVSVAAWRQDPVPAPVVALVDQTAGVMEFCASELYARRMPERARPRIVAV
                     ADATSQIDVSDLTLSTAVIIAQTRSILV"
BASE COUNT          835 a         2084 c         2012 g          965 t
ORIGIN      
        1 tgatgcgtga catcggtcct cctgtcgatc ctcggctgtc ggttgggccg tggctgacgt
       61 tgatgttgct accagagttt tctgtatcga taaagctcgc aaggtctgca cttgctttat
      121 tgataaagat cttgcaaagg cttgatacat accaactagt cggttggtca gggacaggag
      181 tccgaggacc gccaccgccc gcggcaagct cctggccgct gcattcgatc tgatccgagc
      241 gcaaggcctc gccgcgacga gcgtcgacca gctctgcgaa cgggccgggg tcaccaaggg
      301 ggcgttcttt catcatttcg ccaccaagga ggcgctcgcc gtggcggcgg cacaggagtg
      361 gggggccatg acaggagcct tcttcgcagc cgacggctat caccagcagc gcgacccgct
      421 cgagcgcatc ttcgcctacc tcgatctacg ggcctctctc atcgagggcg ggccggcggc
      481 atacagctgc gtggccggca cgatggtcca ggaggcgttt gccaccagcc cggccatcag
      541 agacgcctgc ggcacaatgt ttttcgagca cgccgccact ctcgagtccg acattcaggc
      601 cgccatcgac cagcacgcac cggccctgga ggtcaccgcg cggagcctcg cggtccacat
      661 cctcgtcgtc ctgcacggcg ccttcgtcgt cagcaaggcc ggcgacgatc cccagatcgt
      721 gctcgactcc atcgagcacc tccgccgcta cctgcggcaa ctgttcaccg ccgaagcgaa
      781 tcacccgaag gagaaggaat catgagcacg acgaccaatg cagcgcgcta ccagacctgt
      841 ctctggttcg acgccgacgg cctcgaggca gcagagcacg acacgagtct gctgcccgat
      901 gcggagatca ccggcgtctc gcgctatccc gacgacgtcg acaagggcac gcccggcgcg
      961 gtcatgcagg tcgacttccg cctcggcgac cagcgctatg tcgccctgaa cggcggtccg
     1021 cagttccccc actccgagac ggtctcgctc caggtctacg tcgagaccca ggaggacctc
     1081 gacaggatct gggcgggtct gctcgccggc ggcggcgaag agacgatgtg cgggtggctg
     1141 cgagaccggt tcgggctgtc ctggcaggtc atccccactc gtctgggcga gctgctcgcc
     1201 gaccccgatc ccgaacgcgt gggccgggtg gtgcgccaac tgcaggcgat gaccaagctg
     1261 gacatcgccc cgctcgaggc cgcatacaac gggtgaaccg atcccgcgtg gctcctgccc
     1321 gtcaccgtag cgaaaccggg gtcaggaccc cggttctgct caggtgacac caccggcgcg
     1381 gctgcccccc gcgtccagga cgatggggtg accgtgcggg gtgcgagccg ccgccgcgtg
     1441 ccaggcggcc gttgtgcgga gcaggtcctc ccgcgacagc agctccttgg cggccagcag
     1501 atcgccgagc gcggcagccc aacactcgta atagtcggag ccgtcgggca cggcactggc
     1561 ggcgatccgc gcgcccaggg cctgggccca ttcctcggcg ccgacgaggc cttggtcctg
     1621 aagtgcgacc gtcatcgcga aaacctgtgc ctgccaagga gcttcgaacg gataatcgcg
     1681 gggcggccga ggcgccgggg tcgtcacgac gccgcctcga gatagggctc ccacgcgtcc
     1741 acgcagacgg tcacagtggg atcgctgtcc gggccccaca ggtcggtgcc gctgaactcg
     1801 actgcataga tccactcggg cgtctggtcc gccggctctc cgatcggcac ggcgtcacga
     1861 tcgggaaaga cctgcgaacc gtggaccagc acgatcgtgc cgacctggcc gcgcacgaac
     1921 cggggcagcc gcgtgtgccc ggccgggtgg atcatccggg ctcgcacccg atcgccaacg
     1981 gcgaactggg gcggcgaatc cgtctctcgg gcaaaggaat tggaggcgtc gagggcggac
     2041 ttcatctcag gccacgtgcg cgcccgcgcg gcgaacccgc cgtcgagcag gccgctccgg
     2101 ccgagcagcc cctccggtgc ctcgaaccag atccggtaat agctgctgct cagatagacc
     2161 gccggcggca acgattcgcg ggcgctgcgg gactggtcga tgttccaagc ccccaaggca
     2221 ccggtcgcca aggtgagggc caggacgcga ccctcccatt cggcgtggaa gaccggctcg
     2281 tccggttccg ggcggacggc gccgaagcac tgcataccgc ccacgtcgtg cacgccattc
     2341 acgagaccgt ctcctgcgac ggcgcactcg ccagcccagt tccgatcatc gagtcgcgcg
     2401 tgacgagcgt ggcgagccgc tcggcatccc agccctcagt gtcgtccggc cgcatcggca
     2461 cgaccagata gcgctgttcg gaggtggagt cccacacgcg gatgcgcgtc gaatcgggaa
     2521 gtgtcacacc gaaatcggcg agcacgccgc gcgggtcgcg tactgccttg gcccgatagg
     2581 cgggcgtctt gtaccaaacc ggcggcaacc cgagcaccgg ccagggatag cacgagcaca
     2641 gggtgcacac gaccatgttg tgcacctccg gagtgttcgc cagggcgatc aggtgctcgc
     2701 cggcccggcc gccatagccc atcgacgcga ccgccgtggt ggcatcgcgc acgagccatt
     2761 cacggaagtc tgggtcggtc catgcccggg cgacgacctg cgcaccgcgg tgtgggccga
     2821 tctcgtgttc ataggtctcg acgatcccgt cgagggccgc ggggtcgacg aggccacgct
     2881 cggtcagcag ggtctccagt gcccgaacgc gggcgtccat ctcggaaagc tcgctgcccc
     2941 cggagtgatc gtggtcgtgg tcgtcagcca tgagccacga tctcatgaga cctcaggcgg
     3001 atgccggact ctcgatgcgg gagtcaggcg tgcttgtccg cggcgccctc tcccggctgg
     3061 accgtccact ccggcggttc gtcggtgccg gccttctcgt cggcctcggc gtcctcggcc
     3121 gtctcgtgca ccttgccctg cgcgtggtgc gagggggcgt agacggcata cagccgcagc
     3181 ggctcggagc cggtgttgac gacgtcgtgc cacgtgccgg ccggcacctg gatggaccag
     3241 ccgtcgctga cctcggtctc ggcgaggtcg tccttcgccg ggcccatctt gcacacgccc
     3301 tcgccggcat cgagccgcag gaactggtcc gtctcggggt gggcctccag cccgatcgat
     3361 gcgccgggct cgatggacat cagggtcacc tggagatact tcccggtcca cgcgaccgtg
     3421 cgataggtcg tgttctcctt cgtggccgtc tcgatgtcga agacattggg ctcaggaccg
     3481 ttgtcggtga tcttcatggg gctcctctcg ttgcccacca gcctagactc ggccggcccg
     3541 gctcgagcct cccgcgtgcc ggtgaatccg ctggcgcagc gggacgacaa cgcggctggg
     3601 cggcataccg gaaacgcttc tcggtatgcc gcgcagtcag ttttgcgtga gcgtcagctc
     3661 tcgtcgtcca cgatctcggc gtcgacgggc tcgtcctgcg ggccgtcggc caggatccgg
     3721 tagagcgcgc gccgcgtgtc ctcgaggacc ttggcggcct cggtcatcgt tgcggcgtca
     3781 cgcgtggcgg acaccgcctt ggcggccatc gcgacctgcc cgaaggcctg ccacatctcg
     3841 ccgccgggcc ggccgcccat ggcctcgctg gcgtcggcgg tggccgtttc ccacggagcc
     3901 ggtgcctcgt tgtcctcgac ggcctgctgg ccggcgtcgg tgagccggaa ggccttgcgg
     3961 ccgtcgttgt cgaaggcctc gatcaggccc tcgtcctcga gttgggcgag cgcggggtag
     4021 accgcgccgg agctcggctt ccatgcgccg ctggagcgcg aggcgagttc ctcgatgatc
     4081 tggtagccgt tgcgcggctc ctcggcgatg aggagcaggg cggccaggcg gacatcgccc
     4141 ttgggccggc gcccgcggcg gccaccgggg ccaccgtgac cgcggcggcc acgcccctcg
     4201 ccccgcgggc cgtccgggcc gccgcgccgc ttgccgcggt ccccgtcggg ccggccgaac
     4261 tcgccgtcct cggcccagtc acgccggccg cgcggcccgc gctcccagtc gcgaggtggt
     4321 gcggaggcgt cctgcgggtg ggcggcaccg gcggtggcgt tctcccagat ggcgcgggcc
     4381 atgtcggcgc cctgcgggcc gaagagcccg cccttgccga acgggccgcg cggcccgaag
     4441 gggccgtccg cgccgaagat ctgctcccac gggctgtcgc cctgggggcg gcggccgcgg
     4501 gggccgttgt ggtggccgcg gtgctcgtgc gggccgcgcg gtccgcggtg acggcgctcg
     4561 ccgccgaagt cgtcacggtc ctcgtgactt tcgtagtcgt gtcggtcgtt gctcatggtg
     4621 agcatctcct tgctgccatc gctggcggta tgtcgtgggg ccgcctccgg tggggcggct
     4681 cgcgggatcc gggtgggcga tcccctcgcc cgatcatccg gttcgctggc ttgcagcgac
     4741 atgacaaaga tatgtcgtta tgtcgggcgt gtcaagtccg gcgtcgaacg ccagacaatg
     4801 ggggagtgat gcgtccgaag cccagtggga tcggcgagac tttccacgag ttatcgctgc
     4861 gcgccttcga cctcagtgaa cgcgccggtc gcgcaggctt ggactccaac cgccgccggg
     4921 tccaacggtg gcgcgagcac ctgttcatga tgagtcagat cgccatcgcc gccgggctgg
     4981 cgtggttcct cgggcagcat ctcctcggcc atcagctgcc cttcttcgcc gccgtggcgg
     5041 caatcatctg tctcggcctg tccttcggcc agcgcatcag ccgggtggtc caggtcgccg
     5101 tcggcgtctt cgtcggcgtg ttcgtcggtg acctgttcgt cgccctcgtc ggcaccggcg
     5161 cctggcagat cagcctggtc gtgttcgtcg cgatgtcgat cgccatctgg gtcggcgcga
     5221 agatcctcat ggtcaatcag gcgggcatcc aggccgccac cgtggtgacg ctgttcccca
     5281 acccggacga gggcgtgagc cggtggctcg acgccctgct cggctgcgcc atcgccctcg
     5341 tgttcgctct cgtcgcgccg acctctccgg tgcagaagcc gcggatgaag gccgcccagg
     5401 tgctctacga ggccgccgag acgctgtatg ccgccaccgc cgcactccgg tccggtgacg
     5461 agaacgccgc cgacgaggtc ctggcccggg cccgcagcac cgagggcaag ttgcgcgcgc
     5521 tctccgaggc atcctccgaa ggcatcgcgc tggtgcgcta ttcgccgttg atgcgccaac
     5581 accgggacca cgtccaggcc gtcgccgaga tcgtcgcccc gctcgatcgg atgctgcgca
     5641 atctgcgggt tctcgcccgc cgcgtcagtg tggccgcctg gcgccaggac ccggtgcccg
     5701 cgccggtcgt cgcgctcgtc gatcagacgg cgggcgtgat ggagttctgc gccagcgagc
     5761 tgtatgcccg ccggatgccc gagcgcgccc gtccccgcat cgtcgcggtc gccgacgcca
     5821 catcgcagat cgacgtgagc gacctgaccc tgtccaccgc cgtcatcatc gcccagaccc
     5881 gcagcatcct ggtcga
//
LOCUS       DAOPJG010000201         5890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14551_length_5890_cov_7.416967, whole genome shotgun sequence.
ACCESSION   DAOPJG010000201 DAOPJG010000000
VERSION     DAOPJG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14551_length_5890_cov_7.416967"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..730)
                     /locus_tag="PKU75_14475"
     CDS             complement(65..730)
                     /locus_tag="PKU75_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF82131.1"
                     /translation="MSEPIRVVLVDDDPMVRTGLGLILGGDSGIQIVAEAVDGEDALG
                     VVARERPDVVLMDIRMPRRDGLSATETLLAQPNPPRILVLTTFDADELVLKALRAGAA
                     GFLLKDTPPARLVEAVRAVAAGQPMLSPSVTAQLIAAVAGPAADPEDTSAADARSRLD
                     TLTERELEVAQAIGRGLSNQEIAGELYMSVATVKAHVGRLFTKLGVDNRVQIAILVHD
                     AHK"
     gene            complement(727..1950)
                     /locus_tag="PKU75_14480"
     CDS             complement(727..1950)
                     /locus_tag="PKU75_14480"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPF82132.1"
                     /translation="MPARPEPPAPPIGRWGKIWRVAFVLIVGLVAWVSTATSLSSDTM
                     EVRHLWALIGDPVLGVVAGILTVWRRRWPQRIALAVTLIGVVSSSAGGAGILTLASVA
                     ARRQWRELAWLAPLNVAGGLAVERIYPSTPENETPWPVMLILLLLIVAVVIAVGYAVG
                     GRRELAWERVESAELDAQRRAAQAAAAERTRIAREMHDVLAHRISLVAMHAGALNYRT
                     DLSPEQMGDALGTIETNAHQALADLREVLGVLREPRSLDTDAAPEPPQPEISALPALI
                     AEAEALGSRVTFSDETTGAVPSGLGRTAYRVVQEALTNARKHAPGAVVRVRLAGSPIA
                     GLQIEVRNPRSLQDLDHALPKSGLGLLGLQERVALAGGRLDHGQTADGGYAVTAWLPW
                     PNAPTDTEATSRRSA"
     gene            2092..2988
                     /locus_tag="PKU75_14485"
     CDS             2092..2988
                     /locus_tag="PKU75_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF82133.1"
                     /translation="MITVENLTKRYGQFTAVDDISFEVQPGSVVGFLGPNGAGKSTAM
                     RMMTGLTPPTSGRSTILGRDYHALPNPARHVGVMLDAAAQHPGRTGKEVLHLVGMTIG
                     APKSRVDQAMDMVGLTPAEAKRRVRNYSLGMRQRLGLAGALMGDPQVLILDEPANGLD
                     PQGIHWMRQLLRAYADQGGTVLLSSHLLHEVQIIADELVMIGRGRIVAQGSKAELLGA
                     GGTRVHSLDNDALANALQAKGFEVSGDRSGILVQASPEEVGRAALDAQIVLTELHTAG
                     SEGLEQLFLSLTAADSREPQAA"
     gene            3019..3822
                     /locus_tag="PKU75_14490"
     CDS             3019..3822
                     /locus_tag="PKU75_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF82134.1"
                     /translation="MTAVLDRETAHLTHERPALGEVKGIPFTRLVRVELRKMTDTRAG
                     RWMLIVMAAISLLVVSINLIWGSNADQTFGTFLGLTSFPLMILLPIVGIMAATAEWSQ
                     RTGLVTFTLEPRRGRVLAAKNVAGVLLGLGVIAAAFVAAALAQLLAVWFNGVDADWSM
                     PWKVVLGFVVAMTVFVIQGLAFGFALLITPLAIVASILLPTIWSIVAGISERLNSIGA
                     WLDLNRTVEPLMSGTMTGEQWAHLGTSSLVWIGIPMLIGAWRVLTREVK"
     gene            4001..5779
                     /locus_tag="PKU75_14495"
     CDS             4001..5779
                     /locus_tag="PKU75_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HPF82135.1"
                     /translation="MSREFTKVGVVGLGTMGAGIAEVFARNGIDVVAVEIDDDAINRG
                     KEHLTKSTERAVNRGKLAADDAEALHSRISYSTDLADLAECQLVVEAVPEHMDLKKEI
                     LSALDGVVGPDAILATNTSSLPVTEIAVATANPKRVVGMHFFNPAPVLQFVEVIKTVV
                     TADDVFEDVKALAERIDKKPVVVGDRAGFIANALLFGYLNHAVSMYEGKYATREDLDA
                     AMRFGCGYPMGPLALLDLIGLDTAYEILDTMYKQGRDRLHAPRPIIKQMVSAGLLGRK
                     SGRGFYTYAAPASSEVVPDAQTPSGGAPEGVTLRDVKTVGVIGSGTMATGIVEVFAKA
                     GYPVTVIARGQDKIDGIMASIGKSLAKAVERGRMDQITADDTAARVSGATDRSALKDC
                     DLVLEAIAEDLAIKQDLFTDLDAICKPGAILATTTSSLPIIDLAKVTSRPGDVIGMHF
                     FNPAQVMKLVEVVSTVATDADVIATTVELSKKVGKVPVQCGDRSGFIVNALLFPYLND
                     AIKMLEAHYATADEIDVAMKTGCGLPMGPFELLDVVGLDVSLAIERELYLEFREPGFA
                     PAPLLEHMVQAGYLGRKTKKGFRTYA"
BASE COUNT          932 a         2044 c         2009 g          905 t
ORIGIN      
        1 cgcacaaagt ccgacgtttg gcgagtcagg acccgccaaa cgtcggactt agcggcgggg
       61 ggggtcactt gtgggcatcg tggacgagga tcgcgatctg gacccggttg tcgacgccga
      121 gcttggtgaa caggcggccg acatgggcct tgacggtggc caccgacata tagagctcgc
      181 cggcgatctc ctggttggac agcccccggc cgatggcctg ggccacctcc agttcccgct
      241 cggtcagcgt gtccagccgg ctccgggcat ccgcggcgga ggtgtcctcg gggtccgcgg
      301 cggggccggc gacggcggcg atcagctgtg cggtgacact cggggagagc atcggctggc
      361 ccgcggcgac cgcccgaacc gcttcgacga gccgcgccgg gggcgtgtcc ttgagcagga
      421 agccggccgc gccggcgcgc agcgccttga gcaccagctc gtcggcgtcg aaggtcgtca
      481 gcaccaggat gcgcggcggg ttaggttgtg cgagaagggt ttcagtggcc gataacccgt
      541 cgcgccgggg catccggatg tccatcagga cgacgtcggg acgctcgcgg gccacaacgc
      601 cgagggcgtc ctcgccgtcg acggcctcgg cgacgatctg gatccccgaa tcgccgccga
      661 ggatcagtcc gagccccgtg cgcaccatcg ggtcgtcgtc gacgaggacg acgcggatcg
      721 gctcgctcat gcactcctcc tgctggtcgc ctcggtgtcc gtcggcgcgt tgggccacgg
      781 cagccacgcg gtcacggcat acccgccgtc ggcggtctgg ccgtgatcga gccggcctcc
      841 ggccagcgcg acccgctcct gcagtccgag cagtcccagg cccgacttcg gcaacgcatg
      901 atcgaggtct tgcaggctgc gcggattgcg tacctcgatc tggagtccgg cgatcgggct
      961 gccggcgagc cgcactcgca cgaccgcacc gggcgcgtgc ttgcgagcgt tggtcaaggc
     1021 ctcctgcacc acccggtatg ccgtgcgccc cagtccgctc ggaaccgcgc cggtcgtctc
     1081 gtccgagaac gtcacgcggg agccgagggc ctcggcctcg gcgatgaggg cagggagtgc
     1141 agagatctcg ggctggggtg gttcgggggc cgcatccgtg tcgagagagc gcggctcgcg
     1201 cagcacaccg aggacttcgc gcaggtcggc cagcgcctgg tgggcgttgg tctcgatggt
     1261 cccgagcgca tcgcccatct gttcggggct gagatcggtg cgatagttca gcgctcctgc
     1321 gtgcatcgcg accagtgaga tccggtgtgc gagaacgtca tgcatctccc gcgcgatgcg
     1381 ggtgcgctcg gccgccgcgg cttgagccgc ccgccgctgg gcgtccagtt cggccgactc
     1441 gacgcgctcc cacgccaact cccggcggcc ccccacggca tacccgaccg cgatgacgac
     1501 cgccacgatg agcagcaaga ggatcaacat gaccggccag ggcgtctcgt tctccggcgt
     1561 cgagggatag atgcgctcca cggccagccc gcccgcgaca ttcaggggag ccagccaggc
     1621 gagctcgcgc cattgccggc gagccgcgac cgacgccaga gtcaggatgc cggctccgcc
     1681 ggcgctcgac gacacgacgc cgatgagcgt cacggccagg gcgatccgct ggggccagcg
     1741 gcgccgccat accgtcagga tgccggcgac gacgcccagg acggggtcgc cgatcagcgc
     1801 ccagagatga cgcacctcca tggtgtccga cgacagggac gtggcagtgg agacccaggc
     1861 gacgaggccc acgatcagga cgaatgcgac ccgccagatc ttgccccacc gcccgatggg
     1921 gggtgcgggg ggttcggggc gggcgggcat acgggaaagc gtatgtgtcg aggggctggg
     1981 acgggagtga ccagggtcgt acccccgccc gcgggaatcc gaccaaagtc ggatgccgac
     2041 gggaccgtgg gccgatgtgc ccggtgcctc ccggcggcga gagttgaggg catgatcacc
     2101 gttgagaacc tcaccaaacg ttacggccag ttcaccgccg tcgacgacat cagcttcgag
     2161 gtccagccgg gctcggtcgt cggcttcctc ggaccgaacg gcgcgggcaa gtccaccgcc
     2221 atgcggatga tgaccggtct gacgccgccg acgagcgggc gcagcacgat cctcggccgc
     2281 gactatcacg cactgcccaa cccggcccgc cacgtcggcg tcatgctcga cgccgccgcc
     2341 cagcaccctg gccgcacggg caaggaggtg ctccacctcg tcggcatgac gatcggtgcg
     2401 ccgaagtccc gcgtcgatca ggccatggac atggtcggcc tgacgccggc ggaggccaag
     2461 cgccgcgtgc gcaactactc cctcggtatg cggcagcggc tcggcctcgc cggggccctc
     2521 atgggcgacc cgcaggtgct catcctcgac gaacccgcca acggtctcga cccgcaaggg
     2581 atccactgga tgcgccagct gctgcgcgcg tatgccgatc agggcggcac cgtgctgctc
     2641 tccagccatc tgctgcatga ggtgcagatc atcgccgacg agttggtgat gatcggccgg
     2701 gggcggatcg tcgcccaggg cagcaaggcc gaactgctgg gtgccggcgg cacccgcgtc
     2761 cactccctcg acaacgatgc tctcgcgaat gccttgcagg ccaagggttt cgaggtgtcc
     2821 ggcgaccgct ccggcatcct cgtccaggcg agccccgaag aggtgggacg cgcggccctg
     2881 gatgcacaga tcgtgctgac cgaactgcat accgccggat ccgaaggcct ggagcaactc
     2941 ttcctctccc tcaccgccgc cgactcccgc gagccgcagg ccgcctgagc ggcccgccac
     3001 cgaaaggaca caccggacat gactgccgta ctcgaccgcg aaaccgccca cctgactcat
     3061 gaacgccccg ccctcggcga ggtcaagggc atccccttca cgcgcctggt gcgcgtcgaa
     3121 ctgcgcaaga tgaccgacac ccgggcgggt cgttggatgc tcatcgtcat ggcggcgatc
     3181 tcgctgctcg tcgtgagcat caacctgatc tggggcagca acgccgacca gaccttcggc
     3241 accttcctcg gcctgacctc cttccccctg atgatcttgc tgccgatcgt cgggatcatg
     3301 gccgcgacag cggaatggtc ccagcgcacc gggctggtca ccttcaccct cgagccgcgg
     3361 cggggccggg tcctcgccgc caagaacgtc gccggcgtcc tgctcggtct cggcgtgatc
     3421 gccgctgcct tcgtcgccgc cgcgctcgcg caactgctgg ccgtgtggtt caacggtgtc
     3481 gacgcggact ggtcgatgcc gtggaaggtc gtgctcggct ttgtcgtcgc gatgacggtc
     3541 ttcgtcatcc aggggctggc gttcggcttc gcgctcctga tcacgccgtt ggcgatcgtc
     3601 gcctcgatcc tgctgccgac gatctggtcg atcgtcgcgg ggatcagtga gcggctgaac
     3661 tccatcggcg cctggctcga cctgaaccgc accgtcgagc cgctgatgag cggcaccatg
     3721 acgggcgagc agtgggccca cctgggcacc tcgtcgctcg tgtggatcgg catcccgatg
     3781 ctgatcggcg cgtggcgggt gctgacccgc gaggtgaagt agcgggggcc ctcgcggttc
     3841 ctccggggcg gcccgctgca gggggcgggc cgccccggca tactgcttgt cgtccggcgg
     3901 agccgaaagt tgtcgtaccc aggtgtgagg tctccggggt ggcgactgtc acgcggctga
     3961 ggcgtggccg cggcatacgg gacctgacag actcgaaagc atgtcgcgtg agttcaccaa
     4021 agtcggtgtt gtcggtctgg ggaccatggg tgcgggcatt gcggaagtgt tcgcccgcaa
     4081 cggcatcgat gtcgtcgccg tggagatcga cgacgacgcc atcaaccggg gcaaggagca
     4141 tctgaccaag agcaccgagc gggcggtcaa ccgcggcaag ctcgcggccg atgatgccga
     4201 ggccctgcac tcgcggatca gctactcgac cgatctcgcg gacctggccg agtgtcagct
     4261 cgtcgtcgag gccgtgccgg agcacatgga cctcaagaag gagatcctgt ccgcgctgga
     4321 cggcgtcgtc gggccggacg cgatcctcgc gaccaacacc tcctcgctgc ccgtcaccga
     4381 gatcgccgtc gccaccgcca accccaagcg cgtcgtcggc atgcacttct tcaaccccgc
     4441 gccggtgctg cagttcgtcg aggtcatcaa gaccgtcgtc accgcggacg acgtcttcga
     4501 ggacgtcaag gcgctcgccg agcgcatcga caagaagccg gtcgtcgtcg gggaccgggc
     4561 cgggtttatc gccaatgcgc tgctcttcgg ctatctcaat cacgccgtct cgatgtacga
     4621 gggcaagtac gccacccgcg aagacctcga cgccgcaatg cggttcggct gtggctaccc
     4681 gatggggccg ctcgcgctgc tcgacctgat cggtctcgac acggcatacg agatcctcga
     4741 cacgatgtac aagcagggac gcgaccggct gcacgcgccc cgccccatca tcaagcagat
     4801 ggtcagcgcc gggctgctcg ggcgcaagag cggtcgcggg ttctacacgt atgccgcgcc
     4861 cgccagctcc gaggtcgtgc ccgacgcgca gacgccgtcc gggggcgcac ccgagggcgt
     4921 gaccttgcgg gacgtcaaga ccgtcggggt catcggctcc ggcaccatgg caaccggcat
     4981 cgtcgaggtc ttcgccaagg ccggctatcc ggtcaccgtg atcgctcgcg gccaggacaa
     5041 gatcgacggc atcatggcga gcatcggcaa gtcgctggcc aaggccgtcg aacgcgggcg
     5101 gatggaccag atcaccgccg acgacacggc ggcgcgagtg tcgggcgcca ccgaccgttc
     5161 ggcgctgaag gactgcgacc tggtgctcga ggccatcgcc gaagacctcg ccatcaagca
     5221 ggacctcttc acggacctcg acgcgatctg caagccgggg gccatcctcg cgaccaccac
     5281 ctccagcctg ccgatcatcg acctggccaa ggtcacctcc cgtcctggtg acgtgatcgg
     5341 catgcacttc ttcaaccccg cccaggtgat gaagctcgtc gaggtggtca gcacggtggc
     5401 caccgacgcc gatgtcatcg ccacgacggt cgaactgtcc aagaaggtcg gcaaggtgcc
     5461 ggtccagtgc ggtgaccgct ccggcttcat cgtcaacgcg ctgctcttcc cctatctcaa
     5521 cgacgcgatc aagatgctcg aggcgcacta tgcgacggcc gacgagatcg acgtcgcgat
     5581 gaagaccggc tgtgggctgc cgatgggccc gttcgagttg ctcgacgtcg tcgggctcga
     5641 cgtctccctg gcgatcgagc gcgagctcta tctcgagttc cgcgagcccg gattcgcccc
     5701 ggcacccctg ctggagcaca tggtccaggc cggctatctc gggcgcaaga cgaagaaggg
     5761 cttccgcacc tacgcctgag gtcctcgctc acgagcgttc gagcgtcagg tgagtggcag
     5821 cgacgtcccc atgtcgctga ctctcacgtg acggtcagtc gccggtcgcc gggcatgggc
     5881 gaagatgact
//
LOCUS       DAOPJG010000202         5889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14555_length_5889_cov_8.729517, whole genome shotgun sequence.
ACCESSION   DAOPJG010000202 DAOPJG010000000
VERSION     DAOPJG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14555_length_5889_cov_8.729517"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKU75_14500"
     CDS             <1..987
                     /locus_tag="PKU75_14500"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF82136.1"
                     /translation="VLWAAAVRVAAGADALHLYPWISPLVILILSFIAFTILNPRFAT
                     AGSMGLLIQQMAIVAALAIGQTLVILTAGIDLSVGAITVLAMMVMATLAKDGGMPGLL
                     ALIIGVGLATLAGLLNGILVTRMKLPPFIVTLGTLSIFTAIALLYSGGSSIQASHLPA
                     FLNWLGKGFSIGGLRLTYGVLLVILLYAVVGFVLSQTAWGRHVYAVGDEPEAARLSGV
                     PSRRVLLQVYTVAGFIYGIAAWALIGRSGAATPNAIPTANLDSITAVVIGGTSLFGGR
                     GRLIGTLIGAIIVQTFAFGLSLAGVNQQWRLLAIGLLVIIAVSIDQWIRKVK"
     gene            1081..1836
                     /locus_tag="PKU75_14505"
     CDS             1081..1836
                     /locus_tag="PKU75_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF82137.1"
                     /translation="MRIQWGHVVGLDGVDIDLYKGEVLAVIGDNGAGKSTLIKCLTGA
                     YEPTGGTVYLDGQEVHFKRPQDAREAGIETVYQQLAVIPALDIASNLYLAREERRAGP
                     LGSVLRMLDKRGMARRAGESVQNLGIQTIQNMGQAVENLSGGQRQAVAVARAAAFGSK
                     VVVLDEPTAALGVKESGMVLDMIKQLRESGLGIILISHNMPHVWEAADRIHIQRLGGC
                     AGVITPQSHDMGEGVAIMTGAKKLVSDQLDGAA"
     gene            complement(1912..3189)
                     /locus_tag="PKU75_14510"
     CDS             complement(1912..3189)
                     /locus_tag="PKU75_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HPF82138.1"
                     /translation="MALDITAARPDPALLDLPWRLPLEEWPEEILAALPRGISRHVVR
                     FVKLGGRVLAIKEIKPELAIREYHMLRNLGRLGLPCVEPFAVVQGRLARDGTPLDACL
                     VTRHLQFSLPYRALYSQALRPDTAHRLIDALAVLLVRLHLAGFWWGDVSLSNTLFVRD
                     AGAFAAYLVDAETGELRERLSDGQREHDLDIARVNIAGELFDLEAGELLDGEDPIETS
                     ERLVTRYQELWAELTEVEQFTLNERWRVDERIRRLNDIGFDVDELAMTTDIDGTTISI
                     QPKVVDAGHHSRRLLRLTGLDVEEGQARRLLNDLDAYRVATDQVDTDEELAAHDWLTT
                     IYEPITRAIPPNLRGKLEPAEFFHEILEHRWYMAEVAGHDFRIEDAAIDYVANVLPNK
                     PDEKSVLGADTGAIPILATDTAPLPIVTRSRNR"
     gene            3230..4057
                     /locus_tag="PKU75_14515"
     CDS             3230..4057
                     /locus_tag="PKU75_14515"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HPF82139.1"
                     /translation="MPEPLLIARNPDPDSSLPFIVRIPLGAGIVLRTRETWPRTAKVY
                     CHRHDAAWPDDPDIVETIELRSISRRGPAIDIEADRTREARSQFVLTRARGREMIFWQ
                     TARVAKAARPNVDLPTARAGGLEHLEIVVDTRERYAWKFTHQQATTTKRALPVGDYAS
                     FVDDMLVAVVERKSLEDLANSLTSGRLAYRLADLAGMPRASVVVEDRYSSIFKLGHVR
                     PALVADMLGECQVRWPSVPIVFAENRKLAQEWTFRFLGAAVREHLMGEEAHRLLPED"
     gene            complement(4122..5228)
                     /gene="ugpC"
                     /locus_tag="PKU75_14520"
     CDS             complement(4122..5228)
                     /gene="ugpC"
                     /locus_tag="PKU75_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPF82140.1"
                     /translation="MATVKFDQATRVYPGATRPSVDKLDIDIADGEFLVLVGPSGCGK
                     STSLRMLAGLEEVNGGKIWIGDRDVTNLSPKDRDVAMVFQNYALYPHMTVADNMGFAL
                     KIAGVNKSEIDTRVKEAAKILDLTPYLERKPKALSGGQRQRVAMGRAIVRSPQVFLMD
                     EPLSNLDAKLRVQTRTQIASLQRRLGVTTVYVTHDQVEAMTMGDRIALLKDGILQQCA
                     TPREMYDRPANLFVAGFIGSPAMNLVSVPVTGDGQVKFGSHVLPVDRAALASAGKEVM
                     VGVRPEDVEVTTNDDGMPLTVELVEELGADAYVHAVPKDSAMKMYGEEEGVSKPFVAR
                     VDGRRVPQKGETIYVYPNADHVHVFNAESGQRLG"
     gene            5519..>5889
                     /locus_tag="PKU75_14525"
     CDS             5519..>5889
                     /locus_tag="PKU75_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HPF82141.1"
                     /translation="MHKRALGAAALTSTAALVLVGCGSSGSTTSSSASAGNTASASGS
                     ASASESASASQTAAPEEVDAKDPSRADADLVIWADANRAPYLTEYAQQFADENGVTVA
                     VQIAAETRKQFGDATKVGKGP"
BASE COUNT          901 a         1940 c         2034 g         1014 t
ORIGIN      
        1 gttctctggg cggcggcagt ccgcgttgca gcgggtgcag acgccctgca cctctatccg
       61 tggatcagcc cgctcgtcat cctgatcctc tccttcatcg ccttcacgat cctcaacccc
      121 cggttcgcga ccgccggctc gatgggcctg ctgatccagc agatggcgat cgtggccgca
      181 ttggccatcg gccagacgct ggtgatcctg acggccggca tcgatctgtc cgtgggcgcg
      241 atcacggtgc tggcgatgat ggtcatggcc acattggcca aggacggcgg tatgccgggc
      301 ctgcttgcct tgatcatcgg cgtcgggctc gccacgctcg cgggcctgct caacgggatc
      361 ctcgtgaccc ggatgaaact gccaccgttc atcgtgaccc tcggcacctt gtcgatcttc
      421 accgccatcg cgctgctgta ttcgggcggc tcgagcatcc aggcgtcgca cctgccggcc
      481 ttcctcaact ggctgggcaa gggcttttcg atcggtgggc tgcggctgac ctatggcgtg
      541 ctgctggtga tcctgctgta tgccgtggtc ggcttcgttc tgtcccagac cgcctggggc
      601 cggcacgtgt atgccgtggg tgacgagccg gaagcggcgc gcctgtccgg tgtgccgtcg
      661 cgacgggtgc tgctgcaggt ctacaccgtg gccggcttca tctacggcat cgcggcctgg
      721 gccttgatcg gccggtccgg ggcggccacc cccaacgcca tcccgaccgc caacctggac
      781 tcgatcaccg ccgtggtcat cggcggcacc agcctgttcg gcggccgcgg ccggctgatc
      841 ggcaccctga tcggggccat catcgtccag acgttcgcct tcggcctctc gctggcgggc
      901 gtcaaccaac agtggcggct gctggccatc ggcctgctcg tcatcatcgc ggtctccatc
      961 gaccagtgga tcaggaaggt gaagtaaatg gcagtcgcaa gacttccgct gtcgatcgac
     1021 cacatccccg aggagaagcg atcgatctat gacgccgagc cggtgctctc tggccgcggc
     1081 atgcggatcc agtggggtca cgtcgtcggc ttggacggcg tggacatcga cctgtacaag
     1141 ggcgaggtcc tcgcggtcat cggcgacaac ggcgccggca agtccaccct gatcaagtgc
     1201 ctcacgggcg cctatgagcc gaccggcggc acggtctacc tcgacggtca ggaagtgcac
     1261 ttcaagcgcc ctcaggatgc ccgcgaggca ggcatcgaga cggtctatca gcagctggcc
     1321 gtcattcctg ccctcgacat cgcgagcaac ctctacctcg cgcgtgaaga gcgccgggcc
     1381 ggccccttag ggtcggtgct ccggatgctc gacaagaggg gcatggccag gcgggccggc
     1441 gaatcggtgc agaacctcgg catccagacc atccagaaca tgggtcaggc cgtcgagaac
     1501 ctctccggtg gccaacggca ggccgtcgct gtggcccggg ccgccgcctt cgggagcaag
     1561 gtcgtcgtcc tcgacgagcc gaccgccgcc ctcggggtca aggagtccgg catggtgctc
     1621 gacatgatca agcagctgcg cgagagcggc ctgggcatca tcttgatctc ccacaacatg
     1681 ccccacgttt gggaggcggc agaccgcatc cacatccaac gtcttggcgg ctgtgccggg
     1741 gtcatcaccc cgcagtcgca cgacatgggt gagggcgtgg cgatcatgac cggcgccaag
     1801 aaactggtct cggaccagct ggacggtgct gcctgagctg tctggatcca ctcctgaacg
     1861 cttgaggaag cggcggccgc cacccttcgt gggtggcggc cgctgtcgtg ttcagcgatt
     1921 gcgggaacgc gtgacgatcg gcaggggtgc ggtgtcggtc gcgaggatcg ggatggcgcc
     1981 ggtgtcggcg ccgaggaccg atttctcgtc cggcttgttc ggcaggacgt tggcgacgta
     2041 gtcgatcgcc gcgtcctcga tgcggaagtc gtggccggcc acctcggcca tgtaccaccg
     2101 gtgttcgagg atctcgtgga agaactccgc cggctcgagc ttgccgcgca ggttcggcgg
     2161 gatcgcccgc gtgatgggct cgtagatcgt ggtcagccag tcgtgggcgg ccagttcttc
     2221 gtcggtgtcg acctggtcgg tcgcgacgcg ataggcgtcc aggtcgttga gcaggcggcg
     2281 ggcctgtccc tcctccacgt ccagccccgt caggcgcagc aaccggcgcg aatgatgtcc
     2341 cgcgtcgacg accttgggct ggatgctgat ggtcgtgccg tcgatgtcgg tcgtcatcgc
     2401 gagttcgtcg acatcgaagc cgatgtcgtt gagccggcgg atccgctcat cgacgcgcca
     2461 ccgctcgttg agggtgaact gctcgacctc ggtcagctcg gcccacagtt cctgatagcg
     2521 ggtgaccagt cgttcggagg tctcgatcgg gtcctcgccg tcgaggagtt caccggcctc
     2581 caggtcgaag agttcgccgg cgatattgac ccgggcgatg tcgagatcgt gttcgcgttg
     2641 cccgtccgag agccgttcgc gcaactcgcc ggtctcggcg tcgacgaggt atgccgcgaa
     2701 cgccccggcg tcgcggacga acagggtgtt ggagagcgag acatcccccc accagaagcc
     2761 ggccaggtgc agccgcacga gcagcacggc gagtgcgtcg atgagccggt gggcggtgtc
     2821 gggccgcagg gcctggctgt agagcgcccg gtagggcagt gagaactgca gatgacgcgt
     2881 gaccaggcag gcatcgagcg gggtgccgtc ccgggccagg cggccctgga ccaccgcgaa
     2941 cggctccacg cacggcagcc cgagccggcc caggttgcgc agcatgtgat attcgcggat
     3001 ggccagctcg ggcttgatct ccttgatggc aagcacccgc ccgccgagtt tgacgaaccg
     3061 cacgacgtgc cgggagatgc cgcgggggag cgcggccagg atctcctccg gccactcctc
     3121 caggggcagt cgccagggca ggtcgagcag cgccggatcg gggcgtgccg cggtgatgtc
     3181 gagggccacg gccagattct cccccaccga gtccaatacg gtgggatctg tgccggagcc
     3241 gctgctcatc gctcgcaacc ccgatccgga ctccagcctg cccttcatcg tgcggatccc
     3301 cctcggcgcg gggatcgtct tgcggacccg tgagacctgg ccgcggacgg cgaaggtcta
     3361 ttgccaccgc cacgacgcgg cctggcccga cgacccggac atcgtggaaa ccattgagct
     3421 gcggtcgatc tcgcgccgcg gccccgcgat cgacatcgaa gccgaccgca cccgcgaggc
     3481 gcgctcgcag ttcgtgctga cccgggcccg cggccgggag atgatcttct ggcagaccgc
     3541 acgggtggcc aaggccgcgc gcccgaatgt cgacctcccc acggctcgcg ccgggggcct
     3601 ggagcacctc gagatcgtcg tcgacacccg cgagcgctat gcctggaagt tcacccacca
     3661 gcaggccacg acgaccaagc gggcgttgcc ggtgggggac tatgccagct tcgtcgacga
     3721 catgctggtc gcggtggtcg agcgaaaatc gttggaggac ttggcaaatt cgctcacctc
     3781 cggtcgcctc gcctatcggc tcgccgatct tgccggtatg ccgcgtgcct ccgtcgtcgt
     3841 cgaagaccgg tactcgtcga tcttcaagct ggggcacgtg cgcccggctc tcgtcgccga
     3901 catgctcggc gagtgccagg tccgctggcc cagcgtgccg atcgtcttcg ccgagaaccg
     3961 caagctggcc caggagtgga cgttccgctt cctcggggcc gcggtccgcg agcacctcat
     4021 gggggaggag gcacaccgcc tcctccccga ggattgacgg cccctgcggg cagcgaaaag
     4081 cgcggtcctc ggcaagcgag gaccgcgctc ttcaggggct gtcagccgag gcgctgaccg
     4141 gactcggcgt tgaagacgtg gacgtggtcc gcgttggggt agacatagat cgtctcgccc
     4201 ttctgcggga ccctgcggcc gtcgacgcgg gcgacgaagg gcttggagac gccctcctcc
     4261 tcgccgtaca tcttcatggc ggagtccttc gggacggcgt ggacgtaggc gtcggcgccg
     4321 agttcctcga cgagctcgac ggtcagcggc ataccgtcgt cgttggtcgt gacctcgacg
     4381 tcctcgggac ggacaccgac catgacctcc ttgccggccg acgccagggc ggcgcggtcg
     4441 acggggagca cgtggctgcc gaacttgacc tggccgtcac cggtgaccgg caccgagacg
     4501 aggttcatgg cgggggagcc gatgaagccg gcgacgaaga ggttggccgg gcggtcgtac
     4561 atctcgcgcg gggtggcgca ctgctgcagg atgccgtcct tgagcagggc gatccggtcg
     4621 cccatcgtca tggcctcgac ctggtcgtgg gtgacgtaga cggtggtgac gccgaggcgg
     4681 cgctgcagcg aggcgatctg ggtgcgggtc tgcacgcgca gcttggcgtc gaggttggac
     4741 agcggctcgt ccatgaggaa gacctgcggg gaacgcacga tcgcgcggcc catggcgaca
     4801 cgctggcgct ggccaccgga gagggccttc ggcttgcgct cgaggtaggg ggtcaggtcg
     4861 aggatcttgg ccgcttcctt gacgcgggtg tcgatctcgg acttgttgac cccggcgatc
     4921 ttgagggcga agcccatgtt gtcggcgacc gtcatgtgcg ggtagagcgc gtagttctgg
     4981 aagaccatcg cgacgtcgcg gtccttgggg gagaggttgg tgacatcgcg gtcaccgatc
     5041 cagatcttgc cgccgttgac ctcttcgagg ccggccagca tgcgcaggga ggtggacttg
     5101 ccacagcccg aggggccgac gaggacgagg aactcgccgt cggcgatgtc gatgtcgagc
     5161 ttgtcgacgg aggggcgggt ggcgccgggg tagacccggg tggcctggtc gaacttgacg
     5221 gttgccatga ggcagtactc cttcaccggc aggaacgtgc cggacgatcc gttgtaaagg
     5281 tggaacgcca cgggacatcg gtgtcccgga cctgactagt ctgccgtatg aggccccggg
     5341 acgccagcgt ttgtccgttc aaattccgcc ggtatggccg ttacctttcc gatatccttt
     5401 gcgcagagaa agaaacttct tgcagttcat tgccataact gccagacggg tgtaagatgg
     5461 ctccaccatc gtcagggttg catcgtggcg ccctgcagat ttcggtggag atcaccacat
     5521 gcacaagcgt gcgttgggcg cggccgctct gaccagtacc gccgctctcg tcctggtcgg
     5581 ctgcggcagc agcggttcga ccacctcgtc gagcgcctcc gcgggcaaca ccgcgtccgc
     5641 ctcgggttcc gcctctgcgt ccgaatcggc cagcgccagc cagaccgccg ctcccgaaga
     5701 ggtcgacgcc aaggacccgt cccgtgcgga cgccgacctc gtcatctggg ccgacgccaa
     5761 ccgtgcgccc tacctgaccg agtacgccca gcagttcgcc gacgagaacg gcgtcacggt
     5821 cgccgtccag atcgccgccg agacgcgcaa gcagttcggt gacgccacca aggtcggcaa
     5881 gggcccgga
//
LOCUS       DAOPJG010000203         5888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14563_length_5888_cov_6.444883, whole genome shotgun sequence.
ACCESSION   DAOPJG010000203 DAOPJG010000000
VERSION     DAOPJG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14563_length_5888_cov_6.444883"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..>610)
                     /locus_tag="PKU75_14530"
     CDS             complement(205..>610)
                     /locus_tag="PKU75_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPF82142.1"
                     /translation="GGSFTAMTAIYDTMQYIRPDVQTFVIGQAASAAAVLLSAGAPGK
                     RFALPNARVLIHQPALMGGDYGQASDLEIQANEVLRMREWLEETWAKHSGRTAEQVRR
                     DIERDKILSAAEAKEYGLIDEVLASRKASFED"
     assembly_gap    611..620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<621..873)
                     /locus_tag="PKU75_14535"
     CDS             complement(<621..873)
                     /locus_tag="PKU75_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPF82143.1"
                     /translation="MNTTGMTAGGLHVPGPSSRYILPQFEERTSYGMKRLDPYTKLFE
                     DRIIFLGVQVDDASADDIIAQLIVLESQDPDRDILMYINS"
     gene            complement(903..1559)
                     /locus_tag="PKU75_14540"
     CDS             complement(903..1559)
                     /locus_tag="PKU75_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPF82144.1"
                     /translation="MTDSQERTARTETSVTNPSATTPGGQFGLDDHIYNRLLKERIIF
                     LGSDVRDDNANAICAQMLLLAAEDPKSDIWLYINSPGGSVTAGMAIFDTMNWIPNDVA
                     TVAMGMAASMGQFLLSAGTKGKRYATPHARVMMHQPSGGIGGTASDIKIQAEQMLFIK
                     KQMAELIAQHTGQSLEQIEKDSDRDRWFTAEEAKEYGFVDHVFTRAEDAGADDNPVTD
                     "
     gene            complement(1610..2860)
                     /locus_tag="PKU75_14545"
     CDS             complement(1610..2860)
                     /locus_tag="PKU75_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF82145.1"
                     /translation="MTVLTVESSVARAARGGVFGLFALSGVAFAGWASRIPEAKSALD
                     LTAGQLGATLAAMSVGSVVAMPSTGKLIARLGLRRVLVAAMGAASLGLLLVGSGIDLA
                     HSRALVVAGLFFVGFGIGSWDVGMNLEGTDVERQLSRVIMPHFHAAFSGGTVVSALIG
                     AGLSYAKVSVLAHLIGLVLVIVLVGAWSLGRLLPHEATAAGAAATAQRQAGAKSRSAW
                     TEPRTLLIGLVVFVAAFTEGTANDWLAVAFHEGYELPTWAGVLAFATFLTFMTIGRLV
                     GTALLDRHGRVPVLRVCFALAVVGSALVIFGGPALAYIGAAVWGVGAALGFPVGMSAS
                     ADDPARAAARMSTVATIGYVAFIGGPPMLGFLGDHVGVLRSLLVVGAGAVLALLALPA
                     VRETEAQPADAVDAAPPHTAVRCE"
     gene            2935..4032
                     /locus_tag="PKU75_14550"
     CDS             2935..4032
                     /locus_tag="PKU75_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF82146.1"
                     /translation="MALPRQTSSPVKPRVKRPTLKDVAGHAGVSLSTASLVFSGRGPV
                     SPATADKVRAAAAALSYAGPDPLASSLRHGRAGTVAVVVEGRLTLAFSDPFALSVLDG
                     LATELDTIPAGLLLLARVAGDDERLLTQLAAAAYDAVAFPLCAPKDDPAVDFLAGRGI
                     PMIGAGAPVDPRILHVEVDERGLSAAMARHVTQLGHSRIGHVTLPMGAAAQARILCPA
                     DLDQANYPDARERAYGVLDVAPQARIVEAPDPGVSAGEAAARLLLDAPPDRRPTAIVA
                     QSDLLAAGVIRAAREVGLRVPEDLSVTGVDGLDLPWLDQVLTTVVQDGFAKGRMLGSM
                     IADAMAGRTVRPRPFPTALRIGTTTGPAPAG"
     assembly_gap    4157..4256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4257..5526)
                     /gene="tig"
                     /locus_tag="PKU75_14555"
     CDS             complement(<4257..5526)
                     /gene="tig"
                     /locus_tag="PKU75_14555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPF82147.1"
                     /translation="MKSAAENLTPTRVKLTVEVPYEELKPSIDAAYKSIGSQIAIPGF
                     RKGHVPSRIIDQRMGKGAVLQEAVNEAMPGLYAKAIEESAVRPIGQPEVEITEVPMEE
                     GQTLNFTAEMDVIPDFTLPDLDGIAVTVDDVEVTDEDINTQLDALRTRFGTLVPVERA
                     VGEGDFVSIDLSATIGEEQIDEVTGVSYEVGSGNMLPGMDEALLGMTAGETKTFTAAL
                     AGGDRQGEDAECTVTVQSVKERELPELDDDFAQLASEFDSLDELKANVSEVALRGKKF
                     EQGIQARDKILEHLLENVELEVPEGVVEAEVHAHLEGEGRLEDDEHRAEVQESTRRAM
                     KAQFILDKLVEQDDVEVSQEEFVEYLIMQAQQYRMDPNQFAQAIDANNQVPAMVGEVA
                     RRKALAGVLDRAKITDASGNEVDLDELAPDE"
BASE COUNT          848 a         2007 c         1990 g          933 t
ORIGIN      
        1 ctcggactag ccgacgtcct ggccctgatc gagcctggca gtcgtgccgc cctcgaacgt
       61 ctctgcggcg caacccaacc gatgaccgat gacgagttca ccgcccgcgc cgaagcctgg
      121 cggccctacc ggatgtggat gacattcctc gcacgggccc tggggtgaac cacggcgccg
      181 accgtcgccg tgtcgccccg ggaatcagtc ctcgaaggag gccttgcggg aggcgaggac
      241 ctcgtcgatc aggccgtact ccttggcctc ggcggccgag aggatcttgt cgcgctcgat
      301 gtcgcggcgg acctgctcgg cggttcggcc ggagtgcttg gcccaggtct cctcgagcca
      361 ctcgcgcatg cgcagcacct cgttggcctg gatctccagg tcggacgcct ggccgtagtc
      421 accgcccatg agcgccggct gatggatcag cacgcgcgcg ttgggcagcg cgaaccgctt
      481 gcccggcgca ccggccgaca gcagcaccgc tgcggcggag gcggcctgac cgatcacgaa
      541 ggtctgcacg tccgggcgga tgtactgcat cgtgtcgtag atcgccgtca tcgccgtgaa
      601 cgagccaccg nnnnnnnnnn ggctgttgat gtacatgagg atgtcgcggt ccgggtcctg
      661 ggactcgagc acgatcagct gggcgatgat gtcgtccgcg gacgcatcgt cgacctgcac
      721 gccgaggaag atgatgcggt cctcgaagag cttggtgtag gggtcgaggc gcttcatccc
      781 ataggaggtg cgctcctcga actgcgggag gatgtagcgg ctggacgggc cgggcacgtg
      841 gaggccgccc gccgtcatac cagtggtgtt catgtctgtt ctcgtttctc cgtatgccgc
      901 gttcagtcgg tgaccgggtt gtcgtcggcg ccggcgtcct cggcccgggt gaagacgtgg
      961 tcgacgaagc cgtactcctt ggcctcctcg gcggtgaacc aacggtcgcg gtcggaatcc
     1021 ttctcgatct gctcgaggga ctgcccggtg tgctgggcga tgagctcggc catctgcttc
     1081 ttgatgaaga gcatctgctc ggcctggatc ttgatatcgg aggcggtgcc gccgatgccg
     1141 ccggagggct ggtgcatcat cacccgcgcg tgcggggtgg catagcgctt gcccttggtg
     1201 ccggccgaaa gcaggaactg acccatggac gcggccatcc ccatcgcgac ggtcgcgacg
     1261 tcattgggta tccagttcat cgtgtcgaag atcgccatgc cagcggtcac cgacccgccg
     1321 gggctgttga tgtagagcca gatgtcggac ttggggtcct cggccgcgag cagcagcatc
     1381 tgcgcgcaga tggcgttggc gttgtcgtcg cgcacgtcgg agccgaggaa gatgatgcgt
     1441 tccttgagca gccggttgta gatgtggtcg tcgagcccga actgcccacc cggggtagtt
     1501 gcgctggggt tggtcacgct ggtctccgtc ctggcggtgc gctcctgcga atcggtcatg
     1561 gtgaccctaa cgcgcgctat cgccggttca ctcccgaccc ggccggtgtt cactcacagc
     1621 gcacggcggt atgcggtggc gccgcgtcga ccgcgtccgc gggctgcgcc tcggtctcgc
     1681 gcaccgccgg cagcgcgaga agggccagca cggcaccggc gccgaccacg agcaacgagc
     1741 gcaggactcc gacgtggtca ccgaggaagc cgagcatcgg gggcccgccg atgaacgcga
     1801 catacccgat cgtcgcgacg gtggacatgc gggccgcggc gcgcgcggga tcgtccgccg
     1861 acgcgctcat ccccacgggg aatccgagcg ccgcgcccac gccccagaca gccgccccga
     1921 tataggcgag cgcgggtccg ccgaagatca ccagtgccga ccccacgacg gccagcgcga
     1981 agcacacgcg caacaccggc acccggccgt gccggtcgag cagggcggtg ccgaccaggc
     2041 ggccgatggt catgaaggtc aagaaggtgg caaaggccag cacgcccgcc caggtcggca
     2101 gttcatatcc ctcgtggaag gccacggcga gccagtcgtt ggccgtgccc tcggtgaagg
     2161 cggcaacgaa gacgaccagt ccgatgagca aggtgcgcgg ttcggtccag gccgacctgg
     2221 acttggcccc ggcttgacgc tgcgcggttg ccgcggcgcc cgcggccgtc gcctcgtgcg
     2281 gcaggagccg gcccagtgac cacgcgccga ctaagacgat gacgagcacc agcccgatca
     2341 ggtgggcgag gacgctgacc ttcgcatagg acaggccggc accgatcaga gcggagacga
     2401 cggtgccgcc cgagaaggcc gcgtggaagt gcggcatgat cacgcggctg agttggcgct
     2461 ccacgtcggt gccttcgagg ttcatgccga cgtcccacga tccgatgccg aacccgacga
     2521 agaagagccc cgccaccacg agtgccctgg agtgggcaag gtcgatgccg ctgccgacca
     2581 gcagcagccc gagggatgcg gcccccatcg ccgcgacgag cacgcgtcgc aggccgagcc
     2641 gggcaatgag cttccccgtg gacggcatgg ccaccaccga tcccaccgac atcgcggcga
     2701 gcgtggcacc gagttgaccg gccgtcagat cgagcgccga cttggcctcc gggatccgcg
     2761 acgcccagcc cgcaaaggcc accccggaga gcgcgaacag cccgaagacg cccccgcggg
     2821 cggcgcgggc gacggagctc tcgacggtca ggacggtcat gcattcctcc gggggaatcg
     2881 tggaccgacg cggccgggtc gtgtcggtcg aatcgtttcg actagtatcg ggccatggcc
     2941 ctgccacgtc aaaccagctc cccggtcaaa ccgcgggtca aacgcccgac actcaaggat
     3001 gtcgcgggtc acgcgggcgt gtccctttcc accgcctccc tggtcttcag cggccgcggc
     3061 cccgtctccc cggcgacggc cgacaaggtc cgggccgccg cggccgcctt gtcgtatgcg
     3121 ggtcccgacc cgttggcgtc ctccctgcgc cacgggcgcg ccggcacggt ggccgtggtg
     3181 gtcgaaggcc ggctcaccct cgccttctcc gacccgttcg cactgtctgt cctcgacggg
     3241 cttgccaccg aattggacac gatccccgcc gggctgctgc tgctggccag ggtcgccggc
     3301 gacgacgagc ggctcctgac ccagttggcg gccgcggcat acgacgccgt cgccttcccg
     3361 ctgtgcgcgc cgaaggacga tccggccgtc gacttcctcg ccggccgcgg catcccgatg
     3421 atcggagccg gggcgccggt cgatccgcgc atcctccacg tcgaggtcga cgagcgcggg
     3481 ttgagcgcgg ccatggcccg gcatgttacg cagttgggcc actcccggat cgggcatgtg
     3541 accttgccca tgggtgccgc ggcgcaggcc cggatcctgt gcccggcaga cctcgaccag
     3601 gccaactatc ccgatgcgcg cgaacgcgcc tacggcgtcc tcgacgtcgc cccgcaggcc
     3661 cggatcgtcg aagcgcccga ccccggggtg agtgccggcg aggccgctgc ccgactcttg
     3721 ctggacgccc cacccgaccg tcgcccgacc gcgatcgtcg cccagtcgga cctgctcgcc
     3781 gcaggggtca tccgggcggc ccgggaggtg gggctgcggg tgccggagga cctttcggtc
     3841 accggcgtcg acggactcga tctgccctgg ctcgaccagg tgctgaccac ggtcgtccag
     3901 gacggattcg ccaagggccg catgctcgga tcgatgatcg ccgatgccat ggcgggccgc
     3961 accgtccggc cacgcccctt cccgaccgcg ctgcgcatcg gcaccacgac cgggcccgcg
     4021 cccgcgggct gaacctcagg ccttcgcggc cggctcctcg ccggtggcga cgtcgtcctg
     4081 ggccggctcg gcgtcctcgc cggcgtcggc cgactcgatc tcctcgcccg tctcgactgc
     4141 gtcgatgtcc tcgaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctc
     4261 gtcgggagcc agctcgtcga gatcgacctc gttgccggag gcgtcggtga tcttcgcccg
     4321 gtccaggaca ccggcgagcg ccttgcgccg ggccacctca cccaccatgg ccggcacctg
     4381 gttgttggcg tcgatggcct gggcgaactg gttggggtcc atgcggtact gctgcgcctg
     4441 catgatgagg tactcgacga actcctcctg cgagacctcc acgtcatctt gctccacgag
     4501 cttgtcgagg atgaactggg ccttcatcgc gcggcgggtc gactcctgga cctcggcgcg
     4561 gtgttcgtcg tcctccaggc ggccttcgcc ctcgaggtgg gcgtgcacct cggcctcgac
     4621 gacgccctcg ggcacctcga gctcgacgtt ctcgagcagg tgctcgagga ttttgtcgcg
     4681 ggcctggatg ccctgctcga acttcttgcc gcgcagcgcc acctcactga cattcgcctt
     4741 gagctcatcg aggctgtcga actcggaggc gagctgggcg aagtcgtcgt cgagttcggg
     4801 cagctcacgt tccttgaccg actgcacggt cacggtgcac tcggcgtcct cgccctggcg
     4861 gtcgccaccg gcgagcgcgg cggtgaacgt cttggtctcg ccggcggtca tcccgagcag
     4921 ggcctcgtcc atgccgggca gcatgttgcc cgagccgacc tcatacgaga cgccggtgac
     4981 ctcgtcgatc tgctcctcgc cgatcgtggc cgacaggtcg atggagacga agtcgccctc
     5041 gcccacggcg cgctcgacgg gcacgagggt gccgaagcga gtgcgcaggg catcgagctg
     5101 ggtgttgatg tcctcgtcgg tgacctcgac gtcgtcgacg gtcacggcga tgccgtccag
     5161 gtcgggcagc gtgaagtcgg ggatgacgtc catctcggcg gtgaagttga gggtctggcc
     5221 ctcctccatc ggcacctcgg tgatctccac ctcgggctgg ccgatcgggc gcaccgcgct
     5281 ctcctcgatc gccttcgcgt agaggccggg catggcctcg ttgacggcct cctggagcac
     5341 cgcgcccttg cccatgcgct ggtcgatgat gcggctgggc acgtgcccct tgcggaagcc
     5401 ggggatcgcg atctgggagc cgatgctctt gtaggccgcg tcgatgctcg gcttgagctc
     5461 ctcgtagggg acctcgacgg tcagcttgac ccgggtcggg gtgaggttct cggcggcact
     5521 cttcactgcg ggcactccaa aggatggatc ggcgggattg ttcggtggct cggcaggcgc
     5581 gcacgcaggc gcgccgcagc cagggcttgg atcatcgtaa cggtccgatc gagcgcgccc
     5641 aaaaggagat tggtcggggt tctacaaggg tattccagga aggtctacct aactcgatcc
     5701 ctggaggcgc tccgcacctc gtgcgcacga cgacgccctc cgggggcaac ccacgcctca
     5761 catcgtgaac cgcacgtcca cagggtcgac gtgcggttga gtccggagca acggcgaaca
     5821 ctggcaaagg agtacgacgc tggcttgtca tcggaggcgc tggcagctac tcacggcctc
     5881 tcgaagca
//
LOCUS       DAOPJG010000204         5883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14582_length_5883_cov_8.039979, whole genome shotgun sequence.
ACCESSION   DAOPJG010000204 DAOPJG010000000
VERSION     DAOPJG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14582_length_5883_cov_8.039979"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKU75_14560"
     CDS             <1..335
                     /locus_tag="PKU75_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPF82148.1"
                     /translation="IRSVTLLTRVIADAVADGLIQRSQARANAGAEEAGAPAEEPLAE
                     WERELLGADDAAPATAEATEPTTEDAPLDAPVEETADVVAQAPAEDAVPAEETVAVGA
                     ETTEAPTA"
     gene            384..1211
                     /gene="tsf"
                     /locus_tag="PKU75_14565"
     CDS             384..1211
                     /gene="tsf"
                     /locus_tag="PKU75_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPF82149.1"
                     /translation="MANYTAADIKALREATGAGMMDVKKALDEADGDSVKATEILRVK
                     GLKGVTKREGRTTSNGLVAAKAEAGVGTLVEVLCETDFVAKGQKFIDLADKALAQAVA
                     IGAADAQALLDSDLDGQSVQAILDEANATIGEKIEIKRVARVEAPTVVAYLHKTSPDL
                     PAQIGVLVGITGGDEHVARDVAMHVAAFSPTVMTRDEVDAAVVENERRLAEATAREEG
                     KPEAAMPKIIEGRVNGFFKENVLLEQAFAKDPKKTVAKVLEEAGATLTGFARFRVGA"
     gene            1607..2347
                     /gene="pyrH"
                     /locus_tag="PKU75_14570"
     CDS             1607..2347
                     /gene="pyrH"
                     /locus_tag="PKU75_14570"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPF82150.1"
                     /translation="MAPVPEEALHGAYRRVLLKLSGEVFGGGKIGLAPEVVRGIAQQI
                     AEAVRAGVQVAVVIGGGNFFRGAELQQQGMDRTRADYMGMLGTVMNCLALQDFLEKEG
                     IDTRVQTAITMGQVAEPYIPRRAIRHLEKGRVVIFGAGAGMPFFSTDTVSAQRALEIK
                     CDAVLMSKNGVDGVYDSDPRTNPGAKKLDKVTYAEALQQGLKVVDAAAFSLCMDNGLD
                     MIVFGMEGPGNITRAVRGERIGTLVTAH"
     gene            2476..3033
                     /gene="frr"
                     /locus_tag="PKU75_14575"
     CDS             2476..3033
                     /gene="frr"
                     /locus_tag="PKU75_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPF82151.1"
                     /translation="MIDETLFEAEEKMEKAVEVVKEDFAGIRTGRANPAMFHKLLVDY
                     YGAPTPLQQLASFQTPEARTLLISPFDKGAMKNIEKALRDSDFGANPSNDGNVIRIVL
                     PELTQERRKEYIKIAKHKGEEGKVAIRNVRRHAMHDLDKLIKDGEVGEDDGKRGEKEL
                     DALTKKYVDQVEDLTKRKEAELLEV"
     gene            3071..3937
                     /locus_tag="PKU75_14580"
     CDS             3071..3937
                     /locus_tag="PKU75_14580"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPF82152.1"
                     /translation="MNTPPASPVADPGAAPTNPHGRAGRNLGAAIGVGVGLAVVILAS
                     LILAKDSFVVIIAAAMVVGAWELARALRNKGFQVPLVPVGAGAVGMIVAAHQRGPEAL
                     AIAFGLTCAGVLLWRVIDPRPGSARDTAAGIFTALYPCLLGGFAAMMLAPDDGRSRLI
                     TFLLVTVCSDIGGYAAGVLFGKHPMAPRISPKKSWEGFAGSVFTCAVAGAACLFFFFG
                     QPWWQGALLGAVVAAVATIGDLMESVIKRNLGIKDMSNLLPGHGGLMDRLDSLIMVAP
                     LCWAVLHQVVGG"
     gene            3940..5157
                     /gene="rlmN"
                     /locus_tag="PKU75_14585"
     CDS             3940..5157
                     /gene="rlmN"
                     /locus_tag="PKU75_14585"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPF82153.1"
                     /translation="MATDDELPMPTERPTPGQLTFTAPRRGKPPRHLADLDPAERRAA
                     AHASGMPPFRANQLARHYFEHLSIDAATMTDLPAAQRDQWVADLLPPLLTPVRELQAD
                     RGATIKSVWRLHDGALVESVLMRYPKRVTICISSQAGCGMNCPFCATGQGGLTRNMST
                     AEIVDQVRASAARLRLGDLPPVTARTPGAGLGSEAGDVGEGAAYGPTRISNVVFMGMG
                     EALANYRAAVGAIRRLTDKTPDGFGLSARGITMSTVGLVPGIDKLAAEGIPVTLALSL
                     HAPDDDLRDELVPINTRWKVDEAIDAAYRYFQATGRRVSIEYALIRDINDHAWRADLL
                     GEKLNARGRGWVHVNPIPLNPTPGSKWTASRPGVEQQFVERLRAHGIPTTVRDTRGSD
                     IDGACGQLAAATA"
     gene            5229..>5883
                     /locus_tag="PKU75_14590"
     CDS             5229..>5883
                     /locus_tag="PKU75_14590"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPF82154.1"
                     /translation="MAKEISTVDTVVNLCKRRGFVFPCGEIYGGTRSAWDYGPLGVEL
                     KENIKRQWWRAMVTSRDDIVGLDSSIILPPQVWRASGHVDAFVDPLVECLSCHKRFRL
                     DHLQEAVAEKEAKKGKEIDPDSVEPATIACPNCGTRGQWTEPREFNGLLRTHLGVVDD
                     ESGLHYLRPETAQGIFVNYLNVMNAARMKPPFGIAQTGKSFRNEITPGNFIFRTREFE
                     "
BASE COUNT          982 a         2134 c         1940 g          827 t
ORIGIN      
        1 ccatccgctc ggtcaccctg ctgacccgcg tgatcgccga cgccgtcgcc gacgggctca
       61 tccagcgcag ccaggctcgc gccaacgccg gtgccgaaga ggccggcgct cccgccgagg
      121 agccgcttgc cgagtgggag cgcgaactcc tgggtgccga cgacgccgcc ccggccaccg
      181 ccgaggcgac cgagccgacc accgaggacg cgccgctcga cgcacccgtc gaggagacgg
      241 ccgacgtcgt ggcccaggca ccggccgagg acgccgtgcc cgccgaggag accgtggctg
      301 tcggcgccga aaccaccgag gccccgacgg cctgagtgcc gccgccgcgt ccaccgcata
      361 ccgaaagaag gacgaaatac tccatggcga actacaccgc cgctgacatc aaggccctgc
      421 gcgaggccac cggcgcaggg atgatggacg tcaagaaggc gctcgacgag gccgacggcg
      481 actcggtgaa ggccaccgag atcctgcgcg tcaagggcct caagggcgtc accaagcgcg
      541 agggccgcac gacctccaac ggcctggtgg ccgcgaaggc cgaggccggc gtgggcacgc
      601 tcgtcgaggt gctctgcgag accgacttcg tggccaaggg ccagaagttc atcgacctcg
      661 ccgacaaggc gcttgctcag gcggtcgcca tcggcgccgc cgacgcgcag gccctgctcg
      721 actccgacct ggacggccag tccgtgcagg ccatcctcga cgaggccaac gccaccatcg
      781 gcgagaagat cgagatcaag cgcgtcgcgc gcgtcgaggc cccgaccgtc gttgcctacc
      841 tgcacaagac cagccccgac ctgcccgcgc agatcggtgt cctcgtcggc atcaccggcg
      901 gggacgagca cgtcgcccgc gatgtcgcga tgcacgtcgc cgcgttcagc ccgaccgtga
      961 tgacccgtga cgaggtcgac gccgccgtcg tcgagaacga gcgccgcctc gccgaggcca
     1021 ccgcgcgtga ggagggcaag cccgaggccg ccatgcccaa gatcatcgag ggccgcgtca
     1081 acggattctt caaggagaac gtcctgctcg agcaggcctt cgccaaggac ccgaagaaga
     1141 cggtcgccaa ggtgctcgag gaggccggcg ccaccctgac cgggttcgcc cgcttccgcg
     1201 tcggcgcctg atcggctcac cgctgaaacg gccctgcccg agttcgctcg gggcagggcc
     1261 gtttttcgcg ctcgcggtgg ccggagttgg cgcgcggtgg tgcacaccga cgaggagtac
     1321 cgccgctcac gcggtggggt cgggctgttt gcgcaggtca gcggcttgcg aaaagcccgg
     1381 cgcgggacta ctcgtagctc gtgtgtcccg gccggggtcg atgcggccgg tgtgccagcg
     1441 gcgcccgtgc ccataagagt cgtcatgacc agctcacccc ctcgaccgct tcggagcccg
     1501 cggccggtgt gagaaactga cccgaacccg gggcgacgcg atcgccgccc gctgcccaac
     1561 acccaaggag ctcggcgtga ccactgctgc ccccccggcc cccgaactgg cgccggtccc
     1621 cgaggaggcg ctgcacggcg cataccgccg cgtcctgctc aagctctccg gcgaggtctt
     1681 cggcggcggc aagatcgggc tcgctcccga ggtcgtgcgc ggcatcgccc agcagatcgc
     1741 cgaggccgtc cgggccgggg tgcaggtcgc cgtcgtcatc ggcggcggaa acttcttccg
     1801 cggcgccgaa ctgcagcagc agggcatgga ccgcacccga gccgactaca tgggcatgct
     1861 cgggacagtc atgaattgcc ttgcgctgca agacttcctg gagaaggaag gcatcgacac
     1921 gcgggttcag accgccatca cgatgggtca ggttgccgag ccctatatcc cgcgtcgcgc
     1981 gatccggcac ctcgaaaagg ggcgggtcgt catcttcggc gccggcgccg ggatgccctt
     2041 cttctccacc gacaccgtca gcgcccagcg ggccctggag atcaagtgcg atgccgtgct
     2101 gatgagcaag aacggggtcg acggagtcta tgactccgac ccgcgcacca accccggcgc
     2161 caagaagctt gacaaggtca cctacgccga agcgctgcaa cagggcctga aggtcgtcga
     2221 cgccgccgcc ttcagcctgt gcatggacaa cggcctcgac atgatcgtct tcggcatgga
     2281 gggtccgggc aatatcaccc gggctgtccg gggcgaacgg atcgggaccc tcgtcaccgc
     2341 ccactgaccg cccagccgac tcgagcgccc accgaccgcg cattgcgtcc ggggcgcggc
     2401 gaggtgggag actaggtcac cgcaccgcat acgaccgact gtgacccgcg cgccagcgca
     2461 cccgaaggag ccgacatgat cgacgagacc ctcttcgagg ccgaggagaa gatggagaag
     2521 gccgtcgagg tcgtcaagga ggacttcgcg ggcatccgca ccggccgcgc gaacccggcg
     2581 atgttccaca agctcctcgt cgactactac ggcgctccga caccgctgca gcaactcgcc
     2641 tccttccaga cgcccgaggc gcgcaccctg ctcatcagcc cgttcgacaa gggcgcgatg
     2701 aagaacatcg aaaaggccct gcgcgacagt gacttcggcg ccaacccgag caatgacggc
     2761 aatgtcatcc gcatcgtgtt gcccgagctg acgcaggagc ggcgcaagga gtacatcaag
     2821 atcgccaagc acaagggcga ggagggcaag gttgccatcc gcaatgtgcg ccggcatgcg
     2881 atgcacgacc tcgacaagct catcaaggac ggcgaggtcg gcgaggacga cggcaagcgc
     2941 ggtgagaagg aactcgacgc cctgacgaag aagtatgtcg accaggtcga ggacctgacc
     3001 aagcgcaagg aagccgaact cctcgaagtc tgacggcacc atttaccggc cgccgccttt
     3061 cgaacagcag atgaacactc cgcccgcctc gcccgtggcc gacccgggcg ccgcgcccac
     3121 caatccccat gggcgggccg gccgcaacct cggcgcggcc atcggcgtcg gcgtcggcct
     3181 cgcggtcgtg atcctggcga gcctgatcct cgccaaggac tccttcgtcg tcatcattgc
     3241 cgccgccatg gtggtcgggg cctgggaact cgcccgtgct ctgcgcaaca agggttttca
     3301 ggtcccgctc gtgccggtcg gggccggcgc cgtgggcatg atcgtcgccg cgcatcagcg
     3361 cggcccggag gcgctcgcca tcgccttcgg cctcacgtgc gccggcgtcc tcctgtggcg
     3421 agtgatcgat ccgcgcccgg gcagcgcccg cgacacggca gccggcatct tcaccgccct
     3481 ctatccctgc ctgctcggcg ggttcgccgc gatgatgctc gcgcccgacg acggccgatc
     3541 ccggctgatc acgtttctcc tcgtgaccgt ctgctccgac atcggcgggt atgccgctgg
     3601 cgtcctcttc ggcaagcacc cgatggctcc gcggatcagc ccgaagaaat cctgggaagg
     3661 cttcgccgga tcggtgttca cgtgcgccgt cgccggcgcg gcatgcctgt tcttcttctt
     3721 cgggcagccg tggtggcagg gagcactcct cggggccgtc gtcgccgccg tggcaacgat
     3781 cggcgacctc atggaatcgg tcatcaagcg caacctcggc atcaaggaca tgagcaatct
     3841 ccttccggga cacggtggtt tgatggaccg gctcgactcg ctgatcatgg ttgcccccct
     3901 gtgctgggcg gttctgcacc aggtcgtcgg gggctgaaga tggccaccga cgacgagttg
     3961 ccgatgccga ccgagcggcc gaccccgggt caactgacct tcaccgctcc gcgtcgcggc
     4021 aagccaccgc gacatctggc cgacctcgac ccggccgagc gccgcgctgc tgctcacgcg
     4081 tcgggtatgc cgcccttccg cgccaatcag ctcgctcggc actacttcga gcacctgagc
     4141 atcgacgccg cgacgatgac cgatctgcct gcggcacagc gcgatcaatg ggtcgccgat
     4201 ctcctgcccc cgctgctgac acccgtccgc gagttgcagg ccgaccgtgg ggcgacgatc
     4261 aagtcggtat ggcgcctcca cgacggcgcc ctcgtcgagt cggtgctcat gcgctacccg
     4321 aagcgggtga cgatctgcat ctccagccag gccggctgcg gcatgaactg cccgttctgc
     4381 gcgaccggcc agggcgggct cacccgcaat atgtcgacgg ccgagatcgt cgaccaggtg
     4441 cgcgccagcg ccgcgcgcct gcggctcggg gacctgccgc ccgtcaccgc acgcaccccc
     4501 ggcgctggcc tcgggagcga ggcgggcgac gtgggggagg gcgcggcata cggccccacg
     4561 cggatcagca atgtcgtctt catgggcatg ggcgaggccc ttgccaatta ccgggccgcc
     4621 gtcggagcca tccggcgcct gaccgacaag actcccgacg gcttcggcct gagcgcacgc
     4681 ggcatcacga tgtcgaccgt cggcctggtg ccgggcatcg acaagcttgc ggcagaaggc
     4741 attccggtga ccctggcgct ctcgctgcat gccccggacg acgacctgcg ggacgaactg
     4801 gtgccgatca acacccggtg gaaggtcgat gaggcgatcg acgcggcata ccgctatttc
     4861 caggcgacgg ggcgtcgggt gagcatcgag tacgccctca tccgcgacat caacgaccac
     4921 gcctggcgcg cggatctctt gggggagaag ctcaatgccc gcggccgcgg gtgggtgcac
     4981 gtcaacccga tcccgctcaa cccgaccccc gggtcgaagt ggaccgcctc ccggcccggc
     5041 gtcgagcagc agttcgtcga gcggctgcgg gcgcacggca tacccaccac cgtgcgcgac
     5101 acccgcggct ccgatatcga cggcgcctgc ggccagctcg ccgctgccac cgcctgacgg
     5161 ccgggccacg ccccccgtcg gcgggagcga ttggggcggc atacgcccgg gcggttagcc
     5221 tgtcgcccat ggccaaggag atctccaccg tcgacaccgt cgtcaacctc tgcaagcgcc
     5281 ggggtttcgt cttcccgtgc ggcgagatct acggcgggac gcgctcggcg tgggactacg
     5341 gacccctcgg cgtggagctg aaggagaaca tcaagcgcca gtggtggcgg gctatggtca
     5401 ccagccgcga cgacatcgtc ggcctcgact cctcgatcat cctgccgccc caggtgtggc
     5461 gcgcgtcggg gcacgtcgac gccttcgtcg accctctcgt cgagtgcctc agctgtcaca
     5521 agcgtttccg gctcgaccac ctccaggagg ccgtcgccga gaaggaggcc aagaagggca
     5581 aggagattga ccccgactcg gtcgagcccg ccaccatcgc ctgccccaat tgcggcacgc
     5641 gcggccagtg gaccgagccg cgcgagttca acgggctgct gcgcacccac ctcggcgtcg
     5701 tcgacgacga gtccgggctg cactacctgc gcccggagac cgcgcagggc atcttcgtca
     5761 actacctcaa tgtcatgaac gctgcccgga tgaagccgcc cttcggcatc gcccagaccg
     5821 gcaagtcctt ccgcaacgag atcaccccgg gcaacttcat cttccgcacc cgcgagttcg
     5881 agc
//
LOCUS       DAOPJG010000205         5838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14726_length_5838_cov_8.004842, whole genome shotgun sequence.
ACCESSION   DAOPJG010000205 DAOPJG010000000
VERSION     DAOPJG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14726_length_5838_cov_8.004842"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..425
                     /locus_tag="PKU75_14595"
     CDS             102..425
                     /locus_tag="PKU75_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82155.1"
                     /translation="MSWAASSGAAGRYLSSSADVLSYHTGDRADITWDSTFGFYSNGE
                     AAIEQAVADGTSAAIVLPSGPSGHPAQDAGQAVLQRALASSERYEALTVPGNQAGTWL
                     VYARR"
     gene            complement(459..977)
                     /locus_tag="PKU75_14600"
     CDS             complement(459..977)
                     /locus_tag="PKU75_14600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPF82156.1"
                     /translation="MDGDIERVLARVLRMLGGGGRAARRTSAPHRAEAAAYPGDYMGP
                     LPRITYSPNADGRPDPGEVVWAPVPYQEDSSRSKDRPVLLIGRADPWLLALPLTSQDH
                     DRDAAQEASEGRYWIDVGSGAWDHRGRASEAALHRILRLDPHTIRREGAVLDREVFEQ
                     VRAALEQHYGPG"
     gene            complement(981..1937)
                     /locus_tag="PKU75_14605"
     CDS             complement(981..1937)
                     /locus_tag="PKU75_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPF82157.1"
                     /translation="MTIQQPPAMPPAPPPGMVPVAPPFDDARRREIRGVLRHPLENLT
                     LIVAVLVSLIVMSVSIGSVVDALRNGREPGIYSLLFALAPLVLWLMRGQLMARLRVGG
                     VKITPTQYPEAYAMLQEAAAAFGMKKAPDAYVVLGNGMINAAASGHGFRRFIFVYSDL
                     FEVGGRLKNPDVLRFIIGHEVGHIAAGHASYWRWLGIFGSQIVPFVGPVLSRAQEYTA
                     DNFGYAYCARGSAPAMQTLAVGKYLNPTVDINAIADRAEQEKGFFIWLVNALASHPVL
                     AWRAHALRDRSAPGRLVWRPREPKLLGMPPAHPGLPPAPPRG"
     gene            2072..2725
                     /locus_tag="PKU75_14610"
     CDS             2072..2725
                     /locus_tag="PKU75_14610"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF82158.1"
                     /translation="MIEQIARIEVPGAGPEAYAVLGGVPQALVRPGERMILGFGAADP
                     GPARQQLRRRGLEVDGETVLVGGMPVAILALTDQPECDLTLDHVVVHTSDAERAVADF
                     GGRLGLDLRLDRHAAQWSARMLFFRCGPAVVEVVQPTGDAPHDGPDALWGVAWRTPDL
                     DATVRRLAAGGIAASPVRAGRKPGSRVCSLRERRLGLPTLLIEHTDRSSADRPGDGR"
     gene            2808..4664
                     /gene="lepA"
                     /locus_tag="PKU75_14615"
     CDS             2808..4664
                     /gene="lepA"
                     /locus_tag="PKU75_14615"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPF82159.1"
                     /translation="MSPRARAALQPHATPPELIRNFCIIAHIDHGKSTLADRMLQLTG
                     VVDDRAMRAQYLDRMDIERERGITIKSQAVRMPWEIDGTTYALNMIDTPGHVDFTYEV
                     SRSLAACEGAVLLVDAAQGIEAQTLANLYLAMENDLTIIPVLNKIDLPAAQPEKYAAE
                     LAGLIGCDPEDCLQVSGKTGVGVEALLTEIIRQFPAPVGDPDAPARAMIFDSVYDTYR
                     GVVTYVRVVDGNLNPRERIAMMSTKAQHELLEIGVISPEPVPGKGLGVGEVGYLITGV
                     KDVRQSRVGDTVTNASKPAAQALGGYRDPKPMVFSGLYPIDGSDYPDLREALDKLKLN
                     DAALVYEPETSAALGFGFRCGFLGLLHLEIVRERLEREFNLDLISTQPNVVYSVTMED
                     GSVVTVTNPSEFPGGKIAEVREPVVRATILAPSEFIGAIMELCQNKRGALRGMDYLSE
                     ERVEMRYTLPLAEIVFDFFDQLKSRTRGYASLDYEPDGEQVADLVKVDILLQGQTVDA
                     FSAIVHKDRAYGYGTMMAGKLKELIPRQQFEVPIQAAVGARIIARENIRAIRKDVLAK
                     CYGGDITRKRKLLEKQKEGKKRMKNIGTVEVPQEAFIAALTSDGSSAEKGKK"
     gene            4672..5118
                     /locus_tag="PKU75_14620"
     CDS             4672..5118
                     /locus_tag="PKU75_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82160.1"
                     /translation="MNASQPGWYTQPDGTMRYWDGMQWVGPPRPAWPQDAAPRPPAYG
                     EPGHIQPYAAAGYPPPAGYAPPYGGVAPKNPGISLLASFFVPGLGTMINGEVGKGVAM
                     LVLYVVGIPLAFVLIGIPLMFGVWIWGMIDAYTGAQNWNRRHGIWS"
     gene            5146..>5838
                     /locus_tag="PKU75_14625"
     CDS             5146..>5838
                     /locus_tag="PKU75_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82161.1"
                     /translation="MTEPVEEYAEAPLPAALRPFVASMVGYRLAGFPPGVHVGMPSRH
                     LTVILALDSGIRVSGNGIGNEPRRFGALLGGLHTSPAHVHHDGTQFGVQLALTPLGSR
                     ALLRAPAAAFASGLVEMDDALGGVGDRLRELLALSDGWPRRFDLLSRELLALIDDRRS
                     VDAPTAAAWAMLAGSRGRMPVAAVAEAVGWSRRHLTTRFAREFGPTPKLAGRVLRFEH
                     SRELLGRIPLADI"
BASE COUNT          866 a         2051 c         2031 g          890 t
ORIGIN      
        1 ggcgctacgg tcccgtgggg gcagtcctgg tgctgctgtt cgtgaccggt gtcgctcgca
       61 gtgatgcgct cttcaccagt tgaatcgatg cccggccggt cgtgtcctgg gccgcctcat
      121 cgggggccgc ggggcgttac ctctcgtcat cggcggacgt gctctcctac cacaccgggg
      181 accgggccga catcacctgg gacagcacct tcggcttcta ctccaacgga gaggccgcga
      241 tcgagcaggc cgttgccgac gggacctctg ccgccatcgt cctgccgtcc gggccttccg
      301 ggcatcctgc tcaggacgcc gggcaagcag tcctgcaacg ggccctggcg agcagcgagc
      361 ggtatgaggc gctcaccgtc cccggcaatc aagccggcac gtggctggtc tacgcgcggc
      421 gctagttctc ccgcccgtcc ccggaatcct cgccggcctc accccggtcc gtagtgctgc
      481 tccagggcgg cccgcacctg ctcgaagacc tcccggtcca gcaccgcgcc ctcgcgacgg
      541 atggtgtggg ggtcgagacg caggatgcgg tgcagcgccg cctccgaggc acgcccgcgg
      601 tggtcccaag caccggatcc gacgtcgatc cagtaacgcc cctcggacgc ttcctgcgcg
      661 gcatcgcggt cgtggtcctg actggtcagc gggagggcca gcagccaggg gtcggcacgg
      721 ccgatgagca gcaccggcct gtccttgctc cggctgctgt cctcctggta gggcaccggc
      781 gcccatacca cctcgcccgg gtcgggacga ccgtcggcgt tgggggagta ggtgattcga
      841 ggcagcggcc ccatgtaatc gcctgggtat gccgcggcct cggcccggtg tggtgcgctg
      901 gtccgtcgcg ccgcccggcc gccgccgccc agcatgcgca gcacccgggc gagcacccgt
      961 tcgatgtcgc cgtccaccgc tcagccccgc ggaggcgccg gcggcaggcc gggatgcgcg
     1021 ggcggcatac ccagcagttt gggctctcgc ggccgccaga ccagccggcc cggcgccgac
     1081 cggtcccgca gtgcgtgcgc gcgccaggcg agcaccgggt gcgaggcgag ggcgttgacg
     1141 agccagatga agaagccctt ctcctgttcg gcccggtcgg cgatggcatt gatgtcgacg
     1201 gtcgggttga ggtacttgcc gacggcgagg gtctgcatcg ccggggcgct gccgcgcgcg
     1261 caatacgcat aaccgaagtt gtccgctgtg tactcctgtg cgcgggagag gaccggcccg
     1321 acgaagggca cgatctgcga cccgaagatg cccagccagc gccagtagga cgcgtgcccc
     1381 gcggcgatat ggcccacctc gtggccgatg atgaaccgca gcacgtcggg gttcttcagg
     1441 cgtccgccga cctcgaacag gtcgctgtag acgaagatga accgccggaa accgtgcccg
     1501 gaagccgccg cgttgatcat gccgttgccg agcacgacat aggcgtcggg cgccttcttc
     1561 atgccgaagg ccgcggcggc ctcctggagc atggcgtagg cctcggggta ttgcgtggga
     1621 gtgatcttga ccccaccgac ccgcagccgg gccatcagct gcccgcgcat cagccacagc
     1681 acgagcggcg ccagcgcgaa gagcagcgaa tagatccccg gctcccgccc gttgcgcaga
     1741 gcgtcgacga ccgatccgat cgagacgctc atgacgatca gcgagaccag caccgcgacg
     1801 atcagggtga ggttctcgag ggggtggcgc agcactccgc ggatctcccg ccggcgcgcg
     1861 tcgtcgaacg gcggcgccac cggcaccatc cccggcggcg gtgccggcgg catcgcgggg
     1921 ggctgttgga tcgtcatacg gctggctcct cctgctccct ctccccaacg ggcgggcaca
     1981 accgatcatt ccgcatgcgg gccgggcgcc cgcgggcgga ggcggcgttc acggcatacc
     2041 cttcggcggg tatgggagga ggggagcggt gatgatcgaa cagatcgccc ggatcgaggt
     2101 gcctggcgcc ggtcccgagg cgtatgccgt cctgggcggc gtcccgcagg cgctcgtgcg
     2161 cccgggggag cgaatgatcc tcgggttcgg ggcggccgac ccggggccgg cgcggcagca
     2221 gctgcggcgc cgcgggctcg aggtggatgg tgagacggtg ctggtgggtg gtatgccggt
     2281 cgcgatcctc gccctgaccg accagccgga gtgcgacctg acgctcgatc acgtcgtggt
     2341 gcacacctcg gacgccgagc gcgcggtcgc cgacttcggc ggccggctcg ggctggacct
     2401 gcgcctggac cggcatgcgg cgcagtggtc ggcccgcatg ctgttcttcc gctgcggccc
     2461 ggcggtggtc gaggtcgtgc agccgacggg tgatgcaccg catgacggcc ccgatgcgtt
     2521 gtggggcgtg gcttggcgca ccccagacct cgacgccacc gtccgtaggc tggccgccgg
     2581 ggggatcgcg gcctcgccgg tccgggccgg ccggaagccg ggctcgcggg tctgctcgtt
     2641 gcgggagcgc cggctcgggc tgccgacgct gctcatcgag cacacagacc ggtcgagcgc
     2701 cgaccggccc ggtgacggcc ggtgagcgga tgatcctggg cggacccggc ggcatgggag
     2761 actagggagt cagtcttgat cgtcccgcca cgaaggaacc gccagccgtg tcaccccgcg
     2821 cccgcgccgc gctgcagccg cacgccacgc cgccggagtt gatccgcaac ttctgcatca
     2881 tcgcccacat cgaccacggc aagtccacgc tggccgaccg gatgctccag ctgaccggcg
     2941 tcgtggacga ccgggcgatg cgcgcgcaat atctcgaccg gatggacatc gagcgcgagc
     3001 ggggcatcac gatcaagtcg caggcggtgc ggatgccgtg ggagatcgac gggacgacct
     3061 atgccctgaa catgatcgac acccccgggc acgtcgactt cacctatgag gtctcccgct
     3121 ctctcgccgc gtgcgagggc gcggtcctcc tcgtcgacgc ggcccagggc atcgaggccc
     3181 agaccctcgc caatctctat ctggcgatgg agaacgacct gacgatcatc ccggtgctca
     3241 acaagatcga cctgccggcc gcgcagcccg agaagtatgc ggcggagctg gccggtctca
     3301 tcgggtgcga ccccgaggac tgcctgcagg tatccggcaa gacgggggtg ggtgtcgagg
     3361 cgctgctgac cgagatcatc cgtcagttcc ccgcacccgt cggcgatccc gacgccccgg
     3421 cccgggcgat gatcttcgac tccgtctacg acacctatcg cggcgtcgtc acctatgtcc
     3481 gggtggtcga cggcaacctc aacccgcgcg agcggatcgc gatgatgtcg accaaggccc
     3541 agcacgagct gctcgagatc ggggtcatct ccccggagcc ggttcccggc aagggactcg
     3601 gcgtcggcga ggtcggctat ctgatcaccg gcgtgaagga tgtgcgccag tcccgggtcg
     3661 gcgacaccgt caccaatgcg agcaagccgg cagcgcaggc cctcggcggc tatcgcgacc
     3721 cgaagccgat ggtcttctcg ggtctctatc cgatcgacgg gtccgactat cccgatctgc
     3781 gcgaggcgct cgacaagctc aagctcaacg acgccgccct cgtctacgaa ccagagacct
     3841 ccgcggcgct cgggttcggc ttccggtgcg gcttcctcgg cctgctccac ctcgagatcg
     3901 tccgggagcg tctcgagcgc gagttcaacc tcgacctgat ctccacccag cccaatgtcg
     3961 tctattcggt gacgatggag gacgggtcgg tcgtcacggt gaccaacccg agcgagttcc
     4021 cgggcggcaa gatcgccgag gtccgcgagc cggtggtgcg ggcgacgatc ctcgcgccga
     4081 gcgagttcat cggcgccatc atggagctgt gccagaacaa gcgcggagcg ctgcggggga
     4141 tggactatct ctccgaggag cgggtggaga tgcgctacac cctgcccctg gccgagatcg
     4201 tcttcgactt cttcgaccag ctcaagtccc gcacccgcgg ctacgcctcc ctcgactacg
     4261 aacccgacgg ggaacaggtc gccgacctgg tcaaggtcga catcctgctg caggggcaga
     4321 cagtcgacgc cttcagtgcc atcgtgcaca aggaccgggc ctacggctac ggcacgatga
     4381 tggccggcaa gctcaaggag ctcatcccgc gccagcagtt cgaggtgccg atccaggctg
     4441 ctgtcggggc ccggatcatc gcccgcgaaa acatccgcgc catccgcaag gacgtcctgg
     4501 ccaagtgcta cggcggtgac atcacccgca agcgcaagct gctggagaag cagaaggagg
     4561 gcaagaagcg gatgaagaac atcggcaccg tcgaggtgcc tcaggaggcc ttcatcgccg
     4621 ccctcacctc cgacgggtcg tccgcggaaa agggaaagaa gtagggggcc gatgaacgca
     4681 tcccagcctg gctggtacac ccaacccgat gggacgatgc gctattggga cggtatgcag
     4741 tgggtcggcc caccccggcc cgcctggccc caggatgccg ccccgcgccc cccggcatac
     4801 ggcgaaccgg ggcacatcca gccgtatgcc gccgccggct acccgccgcc tgcgggctac
     4861 gcgccgccgt acggcggggt cgccccgaag aaccccggca tctcgctgct cgcgtccttc
     4921 ttcgtccccg ggctgggcac gatgatcaac ggcgaggtcg gcaagggcgt cgccatgctc
     4981 gtcctctacg tcgtcggcat cccactggcc ttcgtgctca tcgggatccc gttgatgttc
     5041 ggtgtatgga tctgggggat gatcgacgcc tacacgggcg cccagaactg gaaccggcgc
     5101 cacggcatct ggagctaggc cgccgcgtct cctacgctgg ccgtcatgac cgagccggtc
     5161 gaggagtatg ccgaggcgcc cctgcccgct gcattgcgcc ccttcgtcgc ctcgatggtc
     5221 ggctatcgcc tggccggctt cccaccggga gtgcatgtcg gtatgccgtc gcgtcacctg
     5281 acggtgatcc tcgccttgga ttcgggaatc cgggtgtccg gcaacggtat cggcaatgaa
     5341 ccgcgccgtt tcggggcgct gctgggtggg ctgcatacca gcccggcgca cgtccaccac
     5401 gacggcaccc agttcggtgt ccagctggcg ctgacgccgc tcgggtcgcg ggccctgctt
     5461 cgcgcgcccg cagcggcctt cgcctcgggc ctggtcgaga tggacgacgc cttgggcggg
     5521 gtgggggacc ggctgcggga actgttggcg ctgagcgacg gatggccgcg ccgcttcgac
     5581 ctgctcagcc gggagttgct cgctctcatc gatgaccgcc gcagcgtgga cgcgccgacc
     5641 gccgcagcct gggcgatgtt ggccggcagc cgcggccgga tgccggtcgc tgccgtcgct
     5701 gaagccgtcg gctggagccg ccgccacctc accacccgct tcgctcggga attcgggccg
     5761 acgcccaagc tcgccgggcg ggtgcttcgc ttcgagcact cccgagagct gctcgggcgg
     5821 atcccgttgg cggacatc
//
LOCUS       DAOPJG010000206         5832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14752_length_5832_cov_7.529167, whole genome shotgun sequence.
ACCESSION   DAOPJG010000206 DAOPJG010000000
VERSION     DAOPJG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14752_length_5832_cov_7.529167"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKU75_14630"
     CDS             <1..247
                     /locus_tag="PKU75_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPF82162.1"
                     /translation="AHGLQRRIEGPSIAGRRVLIVEDTSTTGASPLDAAAAAREIGAE
                     VVAVATIADRATGAAERFAEAGLDYRNAFSLDDLGLA"
     gene            335..1048
                     /locus_tag="PKU75_14635"
     CDS             335..1048
                     /locus_tag="PKU75_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPF82163.1"
                     /translation="MIALLIILGIIVLFALWAVGVYNGLIKQRNLVQEAWRQIDVELK
                     RRHDLIGNLVETVKGYAAHERGTLEDVMKARSAAMAGGQTPAQQAQSEGMLSAALGRL
                     IAVAEAYPDLKANQNFMALQNELTSTEDRIASARRYYNANVRELNTKVETVPSNVVAG
                     MFNIKREEYFEVEGIERDPVKVDFGQGNYNIPPPSGYNSPQDTAPTQIPTTPPPGQLP
                     NTQVPNPQPGQQAAPPTQN"
     assembly_gap    1122..1221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1235..2419)
                     /locus_tag="PKU75_14640"
     CDS             complement(1235..2419)
                     /locus_tag="PKU75_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF82164.1"
                     /translation="MSTQLSISDRLDLAGLEDLFSEDERAIQQSVRAMCERRIDPYVA
                     DWFERGEIDDIRGLTKELGQLGVLGMHLTGYGCAGMSGVEYGLACMELEASDSGVRSL
                     MSVQGSLAMFAIWRWGSEEHKQGWLPDMAAGTAIGCFGLTEPDHGSDPGSMRTHARRD
                     GDDWILNGSKMWITNGSVADVAVVWAQTDEGIRGFVVPTDTPGFSAPMIKHKMSLRAS
                     VTSELVLDDVRLPGDAVFPEVRGLKGPLSCLNEARYGITWGAMGAARSALETALTYAE
                     QRTQFGRPLGGFQLTQQKLAEMYLEYTKGVLLALHLGRRKDAHTLTPNQVSLGKLNNV
                     RAALDICRTTRTILGANGISLEFPVIRHMNNLESVLTYEGTVEMHTLVVGQALTGEAA
                     FR"
     gene            complement(2416..3591)
                     /locus_tag="PKU75_14645"
     CDS             complement(2416..3591)
                     /locus_tag="PKU75_14645"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPF82165.1"
                     /translation="MPGPLTGLLVADFSRVLAGPLATMTLADLGATVVKVERPGTGDD
                     TRAWGPPWTAHASSYFEALNRGKLSVAWDLGAPADLAKARELASRADVLVENFMTGTL
                     SRFGLDYASVAAVNPGIVYASISGFGSGAGASLPGYDFVAQAVGGLMSITGDAGGEPQ
                     KVGVALVDVLTGKDATIGILAALRERTETGRGGRVEVNLLSSVLGALVNQASAFLATG
                     VPPRAMGNQHPSIAPYEPLRCRDTHLAVACGNDGQFARLCAAIGIPHLATDPRFATNA
                     DRVHNRPELIRCLEERLGTETAAHWEHVVRAAGVAAGPVNSLDSAFAEAEDLGLAPLF
                     SVGDDHPRQVRNPITLSGNVFDVSTPPPDLGEHTALVERWLDSSDPLPTLAPSRSHA"
     gene            complement(3576..5075)
                     /locus_tag="PKU75_14650"
     CDS             complement(3576..5075)
                     /locus_tag="PKU75_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPF82166.1"
                     /translation="MSVPTAAANAIATESTFDDVQAAVAAAVAAVSVLAQVSPAERAG
                     WLDAIAAAVEHHADELAAVADTETGLGRVRLDGEVLRCAAQLRFYADVCAEGSWLGVT
                     IDHATHTGPDLRRMNVSLGPVAVFGASNFPFAFGTLGNDTASALAAGAPVIVKGHPAH
                     PQTHAALMELACAALDAAGGPAGTLSAITGLDAGRQLVLAEGIGAVAFTGSEVAGMTL
                     HRLAATRQQPIPVYAEMGTVNAVVVTTDGADAGDDLARGCVNSFTLGLGQFCTKPGLV
                     LVPAGSGVDVAIANHLTAEPPSGVMLTPGIHEAYDHGIGALLAAGAVPVAQTRATDPL
                     GPNAWVLKADPVLLQPGSRLLAECFGPVILVVEYANRYQRDQLIANLPGGLVATIMSG
                     GKGDEEVPELVSALAPGVGRVTVDTWPTGVVTTWAQQHGGPWPATTDPRATSVGAFAL
                     DRFTRPVVFQSTPNAALPTAVQRGNPWNIVRRVNGRIKVPHEVARCQVP"
     gene            complement(5081..>5832)
                     /locus_tag="PKU75_14655"
     CDS             complement(5081..>5832)
                     /locus_tag="PKU75_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPF82167.1"
                     /translation="GPERCATEALASLEDLVLKQIGANNTAAIVIEPIQGEGGFIVPP
                     AAYLKGVADLARAHGIVLIADEVQTGIARTGEMFACEHDGLVPDIIVTAKGLAGGLPL
                     SGVTGRAELMDAVPPGGLGGTFAGNPVACAAALGVFETIESLDLLSRARHIEAVARPM
                     LEEAAAGNGWIGEVRGRGAMIAVEFVIPGTKDPAPDAAAAVMANCHRQGVVTLQCGTY
                     GNVIRLLPPLVMSDELLHDGLSVLIDAWKGL"
BASE COUNT          892 a         1918 c         2072 g          850 t
ORIGIN      
        1 agcccatggg ctgcagcgac gcatcgaagg accgtccatc gccggccgcc gggtgctgat
       61 cgtcgaggac acctccacga ccggcgcctc ccccctcgac gccgccgcag ccgcccgcga
      121 gatcggggcg gaggtcgtgg ccgtcgcgac catcgccgac cgggccacgg gcgcggcaga
      181 gcgcttcgcg gaggccggct tggactaccg caacgccttc tccctcgacg acctcggact
      241 cgcctgatcc cggctccggc gccgcccgcc cgttaggatc ggcagcgaca ggcgaccggc
      301 cgcgttcacg cgcggcatac gaaacggagt ggcaatgatc gcgttgctca tcatcctcgg
      361 catcatcgtg ctcttcgcgc tgtgggcggt cggggtctac aacgggctga tcaagcagcg
      421 caacctcgtc caggaggcct ggcgccagat cgacgtcgaa ctcaagcggc gccacgacct
      481 gatcggcaac ctcgtcgaga cggtcaaggg gtatgccgcc cacgagcggg gcacgctcga
      541 ggacgtcatg aaggcccgca gcgcggcgat ggccggtggt cagaccccgg cgcagcaggc
      601 ccagtccgaa gggatgctgt ccgccgcgct cggccggttg atcgcggtcg ccgaggccta
      661 ccccgacctg aaggccaacc agaacttcat ggcgctgcag aacgaactga cctccacgga
      721 ggaccgcatc gcctccgccc ggcgctacta caacgccaac gtgcgtgaac tcaacaccaa
      781 ggtcgagacc gtcccgagca acgtcgtcgc cggcatgttc aacatcaagc gcgaggagta
      841 cttcgaggtc gagggcattg agcgcgaccc ggtcaaggtc gacttcggcc aaggcaacta
      901 caacatcccc ccgccctcgg gctacaactc gccgcaggac accgcgccga cccagatccc
      961 gacgaccccg cctccgggcc agctcccgaa cacccaggtc cccaacccgc agccggggca
     1021 gcaggcggcg ccgccgaccc agaactgagc gcttagcgca gggctgacgg cggggtggtt
     1081 cagcggaacg cggcctcgcc ggtcagcgcc tgtccgacca cnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nacggcgggg tggttcagcg gaacgcggcc tcgccggtca
     1261 gcgcctgtcc gaccacgagg gtgtgcatct cgacggtgcc ctcgtaggtc agcaccgact
     1321 ccaggttgtt catgtgccgg atgaccggga actccagcga gatcccgttg gcgcccagga
     1381 tggttcgcgt ggtgcggcag atgtcgagcg ctgcccggac gttgttgagc ttgccgaggc
     1441 tgacctggtt cggcgtgagc gtgtgggcgt ccttgcgtcg cccgaggtgc agcgcgagca
     1501 ggactccctt ggtgtattcg aggtacatct cggcgagttt ctgctgggtc aactggaaac
     1561 cgccgagcgg gcgcccgaac tgggtgcgct gttccgcgta ggtcaacgcc gtctccagcg
     1621 ccgaccgggc agcgcccatc gcgccccagg taatcccgta gcgggcctcg ttgaggcacg
     1681 agagcggacc cttcagtccc cgcacctcgg ggaagaccgc gtcgcccgga agccggacgt
     1741 cgtcgagaac gagttcgctc gtgaccgacg cccgcagcga catcttgtgt ttgatcatcg
     1801 gggcggaaaa gccaggtgtg tcggtgggca ccacgaaccc gcggatccct tcgtcggtct
     1861 gtgcccacac gaccgccacg tcggcgaccg agccgttggt gatccacatc ttcgaaccgt
     1921 tgaggatcca gtcgtcgccg tcgcggcggg cgtgagtgcg catgctgccg gggtcgctgc
     1981 catgatcggg ctcggtgaga ccgaagcagc cgatggcggt gccggccgcc atgtccggga
     2041 gccacccttg cttgtgctcc tccgagcccc agcgccagat ggcgaacatg gcgagggatc
     2101 cctgcacgga catcagcgac cgcacgccgg agtcgctggc ctccagttcc atgcacgcca
     2161 ggccgtactc caccccggac attccggcgc agccgtagcc ggtcaggtgc atgccaagga
     2221 cgcccagctg gccgagctcc ttggtcaggc cgcggatgtc gtcgatctcg ccgcgttcga
     2281 accagtcggc gacatagggg tcgatgcggc gctcgcacat cgcgcgcaca ctctgctgga
     2341 tcgcgcgctc atcctcggag aagaggtctt cgaggccggc gagatcgagt cggtcactga
     2401 tgctgagttg ggtgctcacg cgtggctcct gctgggtgcg agggtgggta aagggtcaga
     2461 cgagtcgagc cagcgctcga cgagtgcggt gtgttcaccg aggtcgggtg gcggcgtgga
     2521 tacgtcgaaa acgttgcccg acaacgtgat cgggttgcga acctgtcgcg gatggtcatc
     2581 cccgactgag aaaagcggtg ccagtcccag gtcctcggcc tcggcgaacg ccgagtcgag
     2641 ggagttgacc gggccggcgg cgaccccggc cgcgcgaacg acatgctccc agtgggcggc
     2701 cgtttcggtg ccgaggcgct cctcgaggca gcggatgagt tccgggcggt tgtggacccg
     2761 gtcggcgttg gtcgcgaagc gcgggtcggt tgccaggtgc ggtatgccga tcgccgcgca
     2821 cagccgtgcg aactggccgt cgttgccgca ggccaccgcc aagtgcgtgt cgcggcatcg
     2881 cagcggctcg tagggcgcga tggacggatg ctggttgccc attgcgcgtg gcgggacgcc
     2941 ggtcgcgaga aatgcgctgg cctggttgac gagggcgccg aggacactcg agagcaaatt
     3001 gacctcgacg cgcccgcccc tcccggtctc ggttcgctcg cgaagggcgg cgaggatacc
     3061 gatcgtggcg tccttgccgg tgagcacgtc gacgagggcg acgccgacct tctgcggctc
     3121 gccgccggcg tcgccggtga tgctcatcag accgccgacc gcttgggcga cgaagtcgta
     3181 gccagggagg gatgccccgg cgccggaccc gaaaccgctg atggacgcgt agacgatccc
     3241 cgggttcacc gccgcgacac tcgcgtagtc caggccgaag cgactcaagg tccccgtcat
     3301 gaagttctcg acgagaacgt ctgcacgaga ggcgagttcg cgtgccttcg ccagatcggc
     3361 cggggcgccg aggtcccagg cgacgctgag tttgccgcgg ttgagtgcct cgaaatagga
     3421 gctggcgtga gcggtccatg ggggccccca ggcgcgggtg tcgtctccgg tgcctggccg
     3481 ctccaccttg acgaccgtgg cgccgaggtc ggcgagggtc atcgtggcga gtggcccggc
     3541 caggacgcgg ctgaagtcgg cgacgagcag accggtcagg ggacctggca cctggccacc
     3601 tcgtgcggga ctttgatccg cccgttgacg cgtcggacga tattccacgg gttgccccgc
     3661 tggacggccg tcggcaaggc ggcgttgggg gtgctctgga agaccacggg acgcgtgaaa
     3721 cggtcgagag cgaaggcacc gacggaggtc gcacgggggt ccgtcgtggc cggccacggc
     3781 ccgccgtgct gttgagccca cgtcgtgacg acgccggtgg gccacgtgtc cacggtgacc
     3841 cggccgacgc ccggcgcgag cgctgacacc agctcgggaa cttcctcatc gcccttcccg
     3901 ccggacatga tcgtggcgac gagaccaccg ggcaggttgg cgatcagctg gtcgcgttgg
     3961 tagcgattgg catactcgac gacgaggatg acaggaccga agcactcggc gaggaggcgt
     4021 gagcccggct gcagcagaac gggatcggcc ttgagaaccc acgcattcgg gccgagagga
     4081 tcggtggccc gcgtctgggc gaccggcacg gcgccggcgg cgagcagggc gccgatgccg
     4141 tggtcgtagg cctcgtggat cccgggtgtg agcatcacgc cggagggtgg ctccgccgtg
     4201 aggtggttcg cgatcgcgac atcgacgccg ctgccggccg ggacgagcac caggccgggt
     4261 ttggtgcaga actggcccaa cccgagggtg aaggagttca cgcaccctcg agcgagatcg
     4321 tcgcccgcgt cggcgccatc tgtggtgacc acgacggcgt tgaccgtgcc catctcggca
     4381 tacacgggaa tgggctgctg ccgggtcgcg gccaggcggt gcagcgtcat cccggccacc
     4441 tcggatcccg tgaaggcgac cgctccgatg ccttcggcga ggaccagctg tcggccggcg
     4501 tccagcccgg tgatggcgga taacgtgccg gcggggccgc cggccgcatc cagtgccgca
     4561 caggcgagct ccatcaacgc tgcgtgcgtc tgcgggtggg ccgggtggcc cttgacgatg
     4621 acgggtgcgc ccgcggcgag ggcgctggcc gtgtcattgc cgagagtgcc gaaggcgaag
     4681 gggaagttgc tggcaccgaa gaccgcgacc gggccgagcg agacgttcat gcggcgcagg
     4741 tcggggccgg tatgggttgc gtggtcgatg gtgacgccga gccaggatcc ttcggcgcac
     4801 acgtcggcgt agaagcgcag ctgggccgcg caccgcagta cctccccgtc gaggcggaca
     4861 cggccgagtc cggtctcggt atcggccacg gccgccagtt cgtcggcatg gtgctcgacc
     4921 gcggcagcga tcgcgtccag ccacccggcg cgttcggccg gagagacctg ggcgaggacc
     4981 gagacggcgg cgacggcggc cgccacggcg gcctggacat cgtcgaatgt ggactccgtg
     5041 gcgatggcgt tcgcggcggc ggtgggcacg ctcatcgtca tcacaacccc ttccaggcgt
     5101 cgatgagcac ggacaggccg tcgtggagga gttcgtcgct catcaccagc ggcggcaaca
     5161 gccggatcac gttgccgtag gtgccgcact gcaaggtgac gacgccttgg cgatggcagt
     5221 tggccatcac cgcggcggcg gcgtcggggg ccgggtcctt ggtgccgggg atgacgaact
     5281 ccaccgcgat catggccccg cggccgcgca cctcaccgat ccagccgttt ccggcagccg
     5341 cctcctcgag catgggccgg gcgacagcct cgatgtgccg ggcccgggac agcaggtcga
     5401 ggctctcgat ggtctcgaag acgccgagcg cggcggcgca ggcgaccggg ttgccggcga
     5461 aggtgccgcc gagccctccg ggcgggacgg cgtccatcag ttccgcccgg cccgtcacac
     5521 cggacagggg aagcccgccg gcgagtccct tcgccgtgac gatgatgtcg gggacgagcc
     5581 cgtcgtgctc gcaggcgaac atctcaccgg tgcgggcgat gccggtctgg acctcgtcgg
     5641 cgatcaggac gattccgtgt gcgcgggcca ggtcggccac gcccttgaga taggcggccg
     5701 gcggcacgat gaagccgcct tcgccctgga tcggctcgat gacgatggca gcggtgttgt
     5761 ttgcgccgat ctgcttcagg acgaggtcct ccagggaggc cagggcctcg gtggcgcagc
     5821 gctccggtcc gg
//
LOCUS       DAOPJG010000207         5813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_14832_length_5813_cov_8.003300, whole genome shotgun sequence.
ACCESSION   DAOPJG010000207 DAOPJG010000000
VERSION     DAOPJG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14832_length_5813_cov_8.003300"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2571
                     /gene="mfd"
                     /locus_tag="PKU75_14660"
     CDS             <1..2571
                     /gene="mfd"
                     /locus_tag="PKU75_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPF82168.1"
                     /translation="REHAAAEGIPWWMLGGFEVDAELAEFGGADEPAARIDAVDVPAY
                     RGRTEDAVRDVAGLINAGWLVQIVTEGPGLAGRIGEVLGERGIPSRRAEELSAGVVAI
                     STGSLGPGFRLEPAKLAVLTETDLMGTATVSGSTKDMRRMPSRRRNQVDPLQLKPGDF
                     VVHEQHGVGRFVEMMQRTVAGATREYLVIEYAASKRGQPADRLFVPTDQLDQVTRYVG
                     GEAPTINRLGGSDWQQTKARARKYVRQIAAELIRLYSARMATTGHAFAPDTPWQRELE
                     DAFAYVETPDQLSTIDEVKADMERPVPMDRLICGDVGYGKTEIAVRAAFKAIQDGKQV
                     AVLVPTTLLVSQHLNTFSERYAGFPVVVKALSRFQSDKEAQEIIAGLKEGSVDLVIGT
                     HRLLGSEVKFKDLGLVVVDEEQRFGVEHKEQLKALRTAVDVLSMSATPIPRTLEMAVT
                     GIREMSTLATPPEERHPILTFVGPYDEKQITAAIRRELLRDGQVFYVHNKVSTIERAA
                     SRLRELVPEARIATAHGKMGEHKLEQVVQDFWERRSDVLVCTTIVETGLDISNANTLI
                     LERADQLGLSQLHQLRGRVGRGRERAYAYFLYPPEKPLTETAIERLQTIAANTDLGAG
                     MQVAMKDLEIRGAGNLLGGEQSGHISGVGFDLYVRLVGEAVANFRGEEEETVTELSVE
                     LPVDAHLPHTYIPGERLRLEAYKKLAQVVSEEELAEVEAELIDRYGPLPEPVSNLVAV
                     ARLRVLARRAGVSEIAVQGNHVRFAPVDLRESQELRLTRLYPKSLYKAGVRTMLVPKP
                     MTARVGGKPLRDLAVLAWASDLIRAVLLDEIAVSGQVAAAGAAQQARGTTRTTERRNR
                     "
     gene            2568..3080
                     /locus_tag="PKU75_14665"
     CDS             2568..3080
                     /locus_tag="PKU75_14665"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPF82169.1"
                     /translation="MRTSKRAAALGLALAGALTLGGCGGATTSDIAAKVNGTVITEDA
                     ARTVAQQIREQFGADAGDFQTPSAVTFLILSKFVLPAAEKAGVHVSEQSIRSELTKVA
                     DPAPETIEMLRTNAAAQQLQQQAPEVLSAVLAEVEKADITVNPRFGKLDVASGALSPA
                     VPNWIESAAP"
     gene            3092..3856
                     /locus_tag="PKU75_14670"
     CDS             3092..3856
                     /locus_tag="PKU75_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HPF82170.1"
                     /translation="MSHDQPPGLTLLLTSPRVAPGLLTRDAWRTLEHASTVLAREPGE
                     PVADAVSVSGVQVSYDASPPPQLARRLVAEAASGSVVWLLSADGDESLTDAIASEVTR
                     LETPPAVEILAGSWDIPGSRLLDVVAVMDRLRSPGGCPWDAKQTHESLAPYLLEEAQE
                     AVEAIESGDRVHLAEELGDVLLQVAFHARVGQEDPEAPFDIDDVAAGLVAKLVRRHPH
                     VFADADAATPEAVEAQWAEIKAAEKAEKAARAAQAD"
     gene            3856..4827
                     /locus_tag="PKU75_14675"
     CDS             3856..4827
                     /locus_tag="PKU75_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPF82171.1"
                     /translation="MAAAARTPERVVIFGGANIDIAARAAGPLDPHSSTPGRVHAGDG
                     GVARNIAENLARLGSPVALVTAFGADADGLGLHERCVGAGIDVRYAVFSQHPSSRYVA
                     LLDEDGELVQGINDMRAIETLTPTDIAAALAAIGAGDLIVVDANLPVPVLTVIAAAAA
                     LAGAPVVAEPVSVAKAGRVLTGVLPRPWLVTPNADEVRILAARGDAAGLDPVADAARL
                     LALGMQHVWVRLGPAGSVLVSGDAAEPVVTDLPARDVEVVDVTGGGDALLAGFLHALL
                     GGADLAAAAAYGHDVAALAVTDRHTVRPDLTPALVERFRTDAKATPP"
     gene            4824..5771
                     /locus_tag="PKU75_14680"
     CDS             4824..5771
                     /locus_tag="PKU75_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPF82172.1"
                     /translation="MTLSTTTPVHPLLQLSEEVAGALAAGGPVVALESTIISHGMPYP
                     DNVAMAREVEQIVRDGGATPATIAILDGIPRIGLSPDQLELLASDEGVIKVSVRDIPY
                     VVATRTHGATTVAATMRLAALAGIRVFVTGGLGGVHQGGQDTMDVSADLTELGATDVA
                     VISAGVKSILDIGRTLEVLETLGVPVVGYGTDEFPSFFSRSSGHRVPMRLDTPEQLAA
                     MMRAKWELGLSGGISVANPVPAEDEIPSEEMDGVIAQALADCAARGITGKDITPYLLG
                     RIVEITGGASLRTNIALVRNNARLGAALAVAFAAPSSLC"
BASE COUNT          878 a         2087 c         2015 g          833 t
ORIGIN      
        1 cgcgaacacg ctgcggcaga aggcattccg tggtggatgc tcggcggctt cgaggtcgac
       61 gcggagctgg cggagttcgg tggcgccgac gagccggccg cccgcatcga cgcggtggac
      121 gtgccggcct atcggggccg caccgaggac gccgtgcgcg atgtcgccgg tctgatcaac
      181 gccggctggc tggtccagat cgtcaccgag ggcccgggtc tcgcgggccg gatcggggag
      241 gtgctggggg agcgcggcat accgtcgcgc cgggcggaag agctctcggc gggtgtcgtg
      301 gcgatctcga ccggatcgct cggccccggt ttccggctcg agccggccaa actcgccgtc
      361 ctcaccgaga ccgacctgat gggcaccgcg acggtctccg gctcgaccaa ggacatgcgc
      421 cgcatgccgt cgcgccgacg caaccaggtc gacccactgc aactcaagcc cggcgacttc
      481 gtcgtccacg agcagcacgg cgtcggccgg ttcgtcgaga tgatgcagcg caccgtcgcc
      541 ggcgccaccc gcgaatacct cgtcatcgaa tatgccgcct ccaagcgcgg ccagcccgcc
      601 gaccggctct tcgtgcccac cgaccagctc gaccaggtca cccggtatgt cggcggcgag
      661 gccccgacga tcaaccggct cggcggcagc gattggcagc agaccaaggc gcgggcgcgc
      721 aagtacgtcc ggcagatcgc cgccgagctg atccgcctct acagcgcccg gatggcgacg
      781 accgggcacg cgttcgcgcc ggacacgccg tggcagcgcg agctcgagga cgccttcgcg
      841 tatgtcgaga cgcccgatca gctctcgacc atcgacgagg tcaaggccga catggagcgg
      901 ccggtgccga tggaccggct catctgcggc gacgtcggct acggcaagac cgagatcgcg
      961 gtgcgcgccg ccttcaaggc gatccaggac ggcaagcagg tggccgtgct cgtgccgacg
     1021 acactgctcg tcagccagca cctgaacacc ttcagcgagc ggtatgccgg cttccccgtc
     1081 gtcgtcaagg cgctctcgcg tttccagagc gacaaggagg cccaggagat catcgccggc
     1141 ctgaaggagg ggtcggtcga cctggtcatc gggacccacc gcctgcttgg gtcggaagtg
     1201 aagttcaagg acctcgggct cgtcgtggtc gacgaggagc agcggttcgg cgtcgagcac
     1261 aaggagcagc tcaaggcgct gcgcaccgcg gtggacgtgc tgtcgatgtc cgccaccccg
     1321 atcccgcgca cgctcgagat ggccgtcacc ggaatccgcg aaatgtccac tctcgcaacg
     1381 cctcccgagg agcggcaccc gatcctcacc ttcgtcgggc cgtatgacga gaagcagatc
     1441 accgcggcca tccggcgtga actgttgcgc gacggccagg tcttctacgt ccacaacaag
     1501 gtctcgacca tcgagcgggc ggcgtcgcgg ctgcgggagc tggtgcccga ggcacggatc
     1561 gccaccgcgc acggcaagat gggcgagcac aagctcgagc aggtcgtcca ggacttctgg
     1621 gagcgccgct cggatgtgct ggtctgcacc accatcgtcg agaccgggtt ggacatctcc
     1681 aacgccaaca ccctcatcct cgaacgcgcc gaccagctcg gcctctcgca gttgcaccag
     1741 ctgcgcggcc gcgtcgggcg cggccgggag cgggcctacg cctacttcct ctatccgccg
     1801 gagaagccgc tcaccgagac cgccatcgag cgcctgcaga ccatcgcggc caacaccgac
     1861 ctcggcgccg gcatgcaggt ggcgatgaag gatctggaga ttcgcggcgc cggcaacctc
     1921 ctcgggggcg agcagtccgg ccacatctcc ggcgtcggtt tcgacctcta tgtccgtctc
     1981 gtcggcgagg cggtcgcgaa cttccgcggc gaggaggagg aaaccgtcac cgagctttcg
     2041 gtggagttgc cggttgacgc ccacctgccg catacctata tccccggcga gcggctgcgg
     2101 ctggaggcct acaagaagct cgcccaggtc gtctccgagg aggagctggc cgaggtcgag
     2161 gccgagctga tcgaccgcta tggcccgctg ccggagcccg tctccaacct cgtggctgtc
     2221 gcccggctgc gggtgctggc gcgccgcgcc ggcgtcagcg agatcgccgt gcagggcaat
     2281 cacgtgcgct tcgcgccggt cgatctgcgc gagagccagg aattgcgcct gacacggctc
     2341 tacccgaagt cgctctacaa ggccggagtc cgtacgatgc tggtgccgaa gccgatgacg
     2401 gcgcgcgtgg gtggcaagcc cctgcgtgac ctcgcggtgc tggcctgggc gagcgatctg
     2461 attcgcgcgg tcttgctcga cgagatcgcc gtctcgggtc aggtcgccgc ggccggcgcg
     2521 gcgcagcagg cgcgcgggac cacccgcaca accgagagaa ggaatcgatg aggacgagca
     2581 agcgagcggc agccctgggc ctggcactgg cgggggcctt gacgctcggc ggctgcggtg
     2641 gcgcgacgac gagcgatatc gccgccaagg tcaacggcac ggtcatcacc gaggacgccg
     2701 cgcgcaccgt ggctcagcag attcgtgagc agttcggggc cgatgccggc gacttccaga
     2761 ctccgtccgc cgtgaccttc ctgatcctgt ccaagttcgt gctgcccgcc gctgagaagg
     2821 cgggggtgca tgtgagcgaa cagtccatcc gctcggagct gaccaaggtc gccgaccccg
     2881 cacccgagac catcgagatg cttcggacca atgcggcggc ccagcaattg cagcagcagg
     2941 cgcccgaggt gctgagcgcc gtcctcgccg aggtggagaa ggcggacatc accgtcaacc
     3001 cgcgcttcgg caagctggac gtggcctccg gtgcgttgtc cccggcggtg ccgaactgga
     3061 tcgagagcgc cgcgccctga gcgggggtgc cgtgagccac gaccaaccgc ccggcctgac
     3121 gctgctgctc accagcccgc gcgtggcccc cggcctgctc acccgcgacg cctggcggac
     3181 tctcgagcac gcgagcacgg tcctggcacg cgagccgggc gaaccggtcg ccgatgcggt
     3241 ctcggtgtcc ggcgtacagg tgtcgtatga cgccagcccg cccccgcagc tggcgcgccg
     3301 tctcgtcgcc gaggccgcgt cggggtctgt cgtgtggctc ctcagcgccg atggcgacga
     3361 gagcctgacc gacgccatcg cgagcgaggt gacgcgcctc gagacgccgc ccgccgtgga
     3421 gatcctcgcg ggctcctggg atatccccgg ctcccggctg ctcgacgtgg tcgccgtcat
     3481 ggaccggctg cgcagccctg gcggctgccc gtgggacgcg aagcagaccc acgaatccct
     3541 tgcgccctat cttctcgagg aggcccagga ggccgtcgag gcgatcgagt ccggcgaccg
     3601 ggtccacctg gccgaagaac tcggagacgt gctcctgcag gtcgccttcc acgcccgcgt
     3661 cggacaggag gaccccgaag ccccgttcga catcgacgac gtcgccgccg gcctggtcgc
     3721 caagctcgtg cgccgccacc cgcatgtctt cgccgacgcc gacgccgcga cccccgaagc
     3781 cgtcgaggcc caatgggccg agatcaaggc cgccgagaag gccgagaagg cggcgcgcgc
     3841 cgcccaggcc gactgatggc cgcggcagcg cggacgcccg agcgtgtcgt catcttcggc
     3901 ggcgccaata tcgacatcgc cgccagggcc gcgggtccgc tcgacccgca cagctccacg
     3961 cccggccggg tgcacgccgg cgacggcggt gtcgcgcgca atatcgccga gaacctcgcc
     4021 cgcctcggtt caccggtcgc cctcgtcacg gcattcgggg cggacgcgga cgggctgggc
     4081 ctccacgaac gctgcgtcgg cgccgggatc gacgtgcgct acgccgtctt ctcccaacac
     4141 ccctcgagtc gctatgtcgc gttgctcgac gaggacggtg aactggtcca gggcatcaac
     4201 gacatgcgcg ccatcgagac cctcaccccc accgacatcg ccgcggcttt ggccgcgatc
     4261 ggcgccggtg acctgatcgt cgtcgacgcg aatctgccgg tgccggtcct cacggtcatc
     4321 gccgcggccg ccgcgctcgc cggcgccccg gtcgtcgcgg agccggtcag cgtggcgaag
     4381 gcgggccggg tgctcaccgg agtgctgccg cggccgtggc tggtcacgcc caatgccgac
     4441 gaggtgcgca tcctcgctgc ccggggggac gctgccgggc tcgacccggt cgccgacgcc
     4501 gcccgcctgc tggctctggg tatgcagcac gtctgggtcc gactcggccc cgccgggtcg
     4561 gtgctggtca gcggggatgc ggcggagccg gtcgtcacgg acttgcccgc ccgggatgtc
     4621 gaggtcgtcg atgtcaccgg cggcggcgac gcgctcctcg ccggcttcct gcacgccctt
     4681 ctcggcggcg ccgacctcgc cgccgcggcg gcatacggcc atgacgtcgc ggcgctggcg
     4741 gtgaccgacc ggcataccgt ccgccccgac ctgaccccag cgctcgtcga gcgcttccgc
     4801 accgacgcga aggcaacccc gccatgaccc tctccacgac cacgcccgtg catccgctgc
     4861 tgcagttgag cgaggaggtg gcgggggcgc tcgccgccgg cgggcccgtc gtcgcactgg
     4921 agtcgacgat catcagccac ggaatgccct atcccgacaa tgtcgcgatg gcgcgggagg
     4981 tcgagcagat cgttcgcgac gggggagcga cgccggcgac gatcgccatc ctcgacggca
     5041 taccgcggat cggcctctcg ccggatcaac tggagctgct cgccagcgac gaaggcgtca
     5101 tcaaggtcag cgtccgcgac atcccctatg tcgtcgccac ccgaacgcac ggtgcgacga
     5161 cggtcgcggc gacgatgcgg ctcgccgcgc tcgccggcat ccgggtcttc gtcaccggcg
     5221 gcctcggcgg ggtgcaccaa ggcggccagg acacgatgga cgtctcggct gacctcaccg
     5281 agctgggcgc caccgatgtc gccgtcatca gtgccggcgt gaagtccatc ctcgacatcg
     5341 ggcggacgtt ggaggtgctg gagaccctcg gcgtgccggt cgtcggctac ggcaccgacg
     5401 agttcccgtc gttcttctcg cgcagctcgg gtcaccgcgt gccgatgcgg ctcgacaccc
     5461 cggaacagct ggccgcgatg atgcgagcga agtgggagct tggtctgtcc ggcggcatct
     5521 cggtcgcgaa tcccgttccg gcagaggatg aaatcccgtc agaagagatg gacggggtca
     5581 tcgcgcaggc gctcgccgat tgcgctgcgc ggggcatcac cggcaaggac atcacgccct
     5641 atctgctggg ccggatcgtc gagatcaccg gcggcgcgtc gctgcggacc aatatcgcgc
     5701 tcgtccgcaa caacgcccgc ctcggcgccg ccctggcggt cgccttcgcc gcccccagta
     5761 gcctctgctg atatgtgact ggtttctgtc aagtggcagg gtgaagcggg gtc
//
LOCUS       DAOPJG010000208         5629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_15477_length_5629_cov_8.450305, whole genome shotgun sequence.
ACCESSION   DAOPJG010000208 DAOPJG010000000
VERSION     DAOPJG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15477_length_5629_cov_8.450305"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..220)
                     /locus_tag="PKU75_14685"
     tRNA            complement(148..220)
                     /locus_tag="PKU75_14685"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Ala,seq:tgc)
     gene            complement(325..507)
                     /locus_tag="PKU75_14690"
     CDS             complement(325..507)
                     /locus_tag="PKU75_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DLW-39 family protein"
                     /protein_id="HPF82173.1"
                     /translation="MKKLLLVVATAVGASILKKNMERSRSEKDLWAEATTPSTTASAE
                     SGKDAWASATDTPGDN"
     gene            complement(540..613)
                     /locus_tag="PKU75_14695"
     tRNA            complement(540..613)
                     /locus_tag="PKU75_14695"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(577..579),aa:Ile,seq:gat)
     gene            complement(688..1134)
                     /locus_tag="PKU75_14700"
     CDS             complement(688..1134)
                     /locus_tag="PKU75_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HPF82174.1"
                     /translation="MSTSVTPAPKTGKTVAAAKTPAVAPRPAGRRVRLTVSRVDPWSA
                     MKVSFLVAIGLGIATVVMIAVLWMLLSGMGVFDQINSMIEKIIGDAETKFDIMDFVGF
                     GRVVSLSVVLGVINVLLITALATLGAFLYNVCASLVGGLQLTLTDD"
     gene            complement(1131..3791)
                     /gene="gyrA"
                     /locus_tag="PKU75_14705"
     CDS             complement(1131..3791)
                     /gene="gyrA"
                     /locus_tag="PKU75_14705"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPF82175.1"
                     /translation="MTELPPEGADAVEPIDLNVEMQRSYIEYAMSVIVARALPDVRDG
                     LKPVHRRIIYAMWDGGYRPDRGFNKCSRVVGEVMGKYHPHGDTAIYDTLVRLVQDWAM
                     RYPLVQGQGNFGSPGNDGAAAPRYTECRMAPLSVELVRDIDEDTVDFGPNYDGKESEP
                     TVLPARFPNLLVNGSAGIAVGMATQIPPHNLREVAEAAQWMLANPDAPREEQLTEVMA
                     RISGPDFPTGALILGRKGIEDAYRTGRGLISMRAIVNVEEIQGRTCLVVTQLPYQVNP
                     DALAQKIAEQVKEGRLQGIADLSDETSGKNGQRLVIVLKRDAVAKVVLNNLYKHTALQ
                     TTFGANMLALVDGVPRTLPVDAFIRHWVDHQIDVIQRRTTYRLRKAERDIHLLRGLLK
                     ALDQLDAVIALIRASRTVEEARAGLMELLDIDEDQARAILDLPLRRLAALERQKIVEE
                     HDELQARIEDYEDILAKPARQRTIISEELAEIVKKYGDERRTEIVPFDGDMNMEDLIP
                     EEDVVVTITRGGYAKRTRVTEYRSQRRGGKGVRGAQLRGEDVVEHFFTTTSHHWLLFF
                     TNLGRVYRAKAYELPDAGRDAKGQHVANLLAFQPDEKIAAVLAIRDYATAPYLVLATR
                     NGLVKKTRLAEFDSPRSGGVIAINLREGDELVGASLVSNVDDLLLVSRKGQSARFNAN
                     DTQLRPMGRATSGVTGMKFRAEDSLLSLSVIPAGTDPDVFVVFESGLAKRSKSSEWTA
                     KGRGILGVAVAKLSERGGDLVGALTVDETDEVLVVMERGNIVRSRVAEVRLTGRNTMG
                     VSFATPRKGDAIVAVARNSETAAEDEEELDGGEPDPTTSGGPGAEASGPHTQIGGDEA
                     DAAEAEASEEVPSDVDDSAESDEDETGGRE"
     gene            complement(3885..>5629)
                     /locus_tag="PKU75_14710"
     CDS             complement(3885..>5629)
                     /locus_tag="PKU75_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HPF82176.1"
                     /translation="LHAGGKFGGGGYKVSGGLHGVGSSVVNALSSRLDVCVRQKGHAF
                     RMTFDHGVPTGPLRQEEETDATGTTITFWPNAGIFETVEFDYETIRARFQQMAFLNKG
                     LTITLIDERIPEVDDDDVDLDNLEEDVEVVVDAEAEEAGEDKVTKAKTVTYRYDGGLV
                     DYVSHLVGSKKSEPVHPEIISIEVEDAARSLSLELAMQWTTAYSESVHTYANTINTHE
                     GGTHEEGFRAALTKLINDFARKQNLLKEKDDNLTGDDVREGLTAVISVKLAEPQFEGQ
                     TKTKLGNSEVKGFVQRAMTDEFGAWLERNPNNGKEIVRKAIQASVARLAARKAREATR
                     KRVLESGGLPGKLRDCQAKDPAVSEVFIVEGDSAGGSAVRARESFNQAILPIRGKILN
                     VEKARIDKILANQEVQALISAFGTGIGEDFDIDKARYHKIILMADADVDGMHIRTLLL
                     TLLFRFMQPLIEHGFVYLAQPPLYRIRWTNSAHEFAFTDRERDGLVAEGQSAGKRLPK
                     ENPIQRYKGLGEMDWQELRETTMDPAERTLLQVTLDNAAAADEIFSILMGEDVESRRS
                     FIQRNARDVRFLDI"
BASE COUNT          853 a         1863 c         1915 g          998 t
ORIGIN      
        1 ccgcggttcg gcacggcgca caggacgcac catacccatc actgataact accgcatgtc
       61 taccgacggc gaaggctcca ggatcactcc tcgggaagac cagaggccgc atccctggcg
      121 ggaaacggcc tctgaactgc ggttttgtgg agactagggg attcgaaccc ctgacatccg
      181 ccttgcaaag gcggcgctct accagctgag ctaagtcccc taggtgggga tatgaagttg
      241 tgagagcccc tgcttgcaaa gcaggtgcgc taccaattgc gccatggccc cggtgcgcgc
      301 agagccgtgc gcgcggcggg agtttcagtt gtcgccgggg gtgtccgtcg cggaggccca
      361 ggcgtccttg ccggactcgg ccgaagccgt ggtggacggg gtggtcgcct cggcccacag
      421 gtccttctcg gagcgggagc gctccatgtt cttcttcagg atgcttgcgc cgacagcggt
      481 ggcgacaacc aggagcagct tcttcatgga ggattcccct atcgatcggc tggtgtgggt
      541 gggcctaaca ggacttgaac ctgtgacctc ttccttatca gggaagcgct ctaaccgtct
      601 gagctatagg ccccagccag gcagcgtccg acaaccagac gcgacgcacg aggttacctc
      661 acgcgtgcac gcgccaccaa aacgagatca gtcgtcggtc agcgtcagct gcaggccacc
      721 gacgagcgag gcgcacacgt tgtagaggaa cgcgccgagc gtggcgagcg cggtgatcag
      781 caacacgttg atgacaccga gcaccaccga caacgacacc acgcgcccga acccgacgaa
      841 gtccatgatg tcgaacttcg tctcggcgtc cccgatgatc ttctcgatca tgctgttgat
      901 ctggtcgaag acgcccatcc ccgacagcag catccacagc acggcgatca tgacgacggt
      961 ggcgatgccc aggccgatcg cgacgaggaa ggagaccttc atcgccgacc acgggtcgac
     1021 gcgcgagacg gtcagccgga cgcggcgccc ggcgggccgg ggtgcgacgg cgggggtctt
     1081 ggcagcggcg acggtcttgc ccgtcttcgg cgccggggtg acgctcgtac tcactctcga
     1141 cctccggtct cgtcctcgtc cgactcggcc gagtcgtcca cgtcagacgg tacctcctcg
     1201 ctcgcctccg cctcggctgc gtccgcctcg tcgccaccga tttgggtatg cggaccgctc
     1261 gcctccgcgc cgggaccgcc gctcgtcgtg gggtcgggtt cgcccccgtc gagctcctcc
     1321 tcgtcctcgg ctgcggtctc ggagttgcgt gccaccgcga cgatggcgtc gcccttgcgc
     1381 ggggtggcga aggagacgcc catggtgttg cggccggtca ggcgcacctc ggcgacgcgg
     1441 gagcgaacga tattgccccg ctccatgacc acgagcacct cgtccgtctc gtccacggtg
     1501 agcgcgccga cgaggtcgcc gccgcgctcg gacagcttcg ccacggccac cccgaggatg
     1561 ccgcggccct tggccgtcca ctccgagctc ttcgaccgct tggccagacc cgactcgaag
     1621 accacgaaga cgtccgggtc ggtgccggcc gggatgacgc tcagcgacag cagcgagtcc
     1681 tcggcgcgga acttcatacc cgtgacaccc gaggtggccc ggcccatcgg acgcaactgg
     1741 gtgtcattgg cgttgaagcg cgccgactga cccttgcggg agaccagcag caggtcgtcg
     1801 acattcgaca cgagcgaggc cccgacgagc tcgtcgccct cgcgcaggtt gatggcgatc
     1861 acgccgccgc tgcgcgggga gtcgaactcc gcgagccggg tcttcttgac caggccgttg
     1921 cgggtcgcca acacgaggta gggggccgtc gcatagtcgc ggatcgcgag cacggcagcg
     1981 atcttctcgt ccggctggaa cgccagcagg ttggcgacgt gctggccctt ggcgtcgcgg
     2041 ccggcgtcgg gcaactcata cgccttggcc cggtagaccc ggcccaggtt ggtgaagaac
     2101 agcagccaat ggtggctggt cgtcgtgaag aagtgctcga cgacgtcctc cccgcgcaac
     2161 tgggcaccgc gcacgccctt gccgccccgt cgctgcgagc gatactcggt gacccgggtc
     2221 cgcttggcat acccgccgcg ggtgatggtg acgaccacat cctcctcggg gatgaggtcc
     2281 tccatgttca tgtcgccgtc gaacggcacg atctcggtgc gccgctcgtc gccgtacttc
     2341 ttgacgatct cggccagttc ctcgctgatg atcgtccgct gccgcgccgg cttggcgagg
     2401 atgtcctcgt agtcctcgat gcgcgcctgc aactcgtcgt gctcctcgac gatcttctgc
     2461 cgctccaggg cggccaggcg ccgcagcggc aggtcgagga tcgcccgcgc ctggtcctcg
     2521 tcgatgtcga gcagttccat caacccggcc cgggcttcct cgaccgtgcg cgaggcccgg
     2581 atcaacgcga tgaccgcgtc cagctggtcg agggccttga gcagtccgcg cagcaggtgg
     2641 atgtcgcgtt cggccttgcg caggcggtat gtcgtgcggc gctggatgac gtcgatctgg
     2701 tggtcgaccc agtggcggat gaaggcgtcg accggcagcg tgcgcggcac cccgtcgacc
     2761 agggcgagca tgttggcgcc gaaggtggtc tgcagcgcgg tgtgcttgta gaggttgttg
     2821 aggacgacct tggccaccgc gtcgcgtttg agcacgatga ccagccgctg gccgttcttg
     2881 ccggaggtct cgtcgctgag gtcggcgatg ccctgcagcc ggccctcctt gacctgctcg
     2941 gcgatcttct gcgcaagggc gtccggattg acctggtagg gcagctgggt caccaccagg
     3001 caggtgcgcc cctggatctc ctcgacgttg acgatcgcgc gcatcgagat caggccgcgt
     3061 ccggtgcggt aggcgtcctc gattcccttg cgccccagga tcaacgcacc cgtcgggaag
     3121 tccggcccgg agatccgcgc catgacttcg gtcagctgct cctcgcgggg cgcgtccggg
     3181 ttggcgagca tccactgggc cgcctccgcg acctcgcgca ggttgtgcgg cgggatctgg
     3241 gtcgccatac ccacggcaat gccggccgag ccgttgacga gcaggttggg gaagcgggcc
     3301 ggcagcaccg tcggctcgga ttccttgccg tcgtagttgg gcccgaagtc gacggtgtcc
     3361 tcgtcgatgt cgcgcacgag ttcgaccgac agcggcgcca ttcggcactc ggtgtatcgc
     3421 ggcgcggccg ccccgtcgtt gcccgggctg ccgaagttgc cctgcccctg gacgagcggg
     3481 tagcgcatcg cccagtcctg cacgagccgc acgagggtgt cgtagatcgc cgtgtcaccg
     3541 tgcgggtggt acttgcccat cacctcgccg accacgcgcg aacacttgtt gaacccacgg
     3601 tccggccggt agccgccgtc ccacatcgcg tagatgatcc gccggtgcac cggcttgagc
     3661 ccatcgcgca catccggcag cgcccgcgcg acgatgaccg acatcgcgta ctcgatgtag
     3721 ctgcgctgca tctcgacgtt caggtcgatc ggctcgaccg cgtcggcgcc ctcgggagga
     3781 agttcagtca tgagtgatcc ttgaatgaag catgcgaacg aagaagcgaa aggaaagcgg
     3841 gatcgctaaa cctcggagcg cggaggtgac aacgcggcat accgtcagat atccaggaac
     3901 cgcacatcgc gcgcgttgcg ctggatgaac gagcgacgcg actccacgtc ttcgcccatc
     3961 aggatcgaga agatctcgtc ggccgcggcg gcgttgtcga gggtgacctg caggagcgtc
     4021 cgctcggcgg ggtccatggt cgtctcgcgc agttcctgcc agtccatctc gcccagaccc
     4081 ttgtagcgct ggatcgggtt ctccttgggc agccgcttgc cggcggactg gccctcggcg
     4141 acgaggccgt cgcgctcgcg gtcggtgaag gcgaactcgt gagccgagtt ggtccaccgg
     4201 atccgataga gcggcggctg cgcgaggtag acgaagccgt gctcgatgag cggctgcatg
     4261 aagcggaaga gcagcgtcag cagcagggtg cggatgtgca tgccgtcgac atcggcgtcg
     4321 gccatcagga tgatcttgtg atagcgggcc ttgtcgatgt cgaagtcctc gccgatgccg
     4381 gtgccgaacg ctgagatcag cgcctgcacc tcctggttgg cgaggatctt gtcgatgcgg
     4441 gccttctcga cgttgaggat cttgccgcgg atcggcagga tggcctggtt gaaggattcg
     4501 cgcgcgcgca ccgccgagcc gccggcggag tcaccctcga cgatgaagac ctcggagacg
     4561 gccgggtcct tggcctggca gtcccgcagc ttgccgggca ggccgccgga ttcgaggacg
     4621 cgtttgcggg tggcctcgcg ggccttgcgg gcggccagcc gggccacgga cgcctggatc
     4681 gccttgcgga cgatctcctt gccgttgttg gggttgcgct cgagccaggc gccgaactcg
     4741 tcggtcatgg cccgctggac gaaacccttg acctcggagt tgccgagctt ggtcttggtc
     4801 tgcccctcga actgcggctc ggcgagcttg acggagatga cggcggtcag gccctcgcgg
     4861 acgtcgtcgc cggtgaggtt gtcgtccttc tccttgagga ggttctgctt gcgggcgaag
     4921 tcgttgatga gcttggtcag cgccgcccgg aaaccctcct cgtgcgtgcc gccctcgtgg
     4981 gtgttgatcg tgttggcata ggtgtgcacc gactcgctgt atgccgtggt ccactgcatc
     5041 gccagctcca ggctgaggct gcgcgctgcg tcctccacct cgatgctgat gatctcgggg
     5101 tgcaccggct cggacttctt ggacccgacc aggtgcgaga cgtagtcgac caggcccccg
     5161 tcataccggt aggtcaccgt cttggccttg gtgaccttgt cctcgccggc ttcttcggcc
     5221 tcggcgtcga cgacgacctc gacatcctcc tcgaggttgt ccaggtcgac atcgtcgtcg
     5281 tcgacctcgg ggatgcgctc gtcgatgagc gtgatcgtca ggcccttgtt gaggaaggcc
     5341 atctgctgga agcgggcccg gatcgtctcg tagtcgaact cgacggtctc gaagatgccc
     5401 gcgttgggcc agaaggtgat cgtggtgccg gtggcgtcgg tctcctcctc ctgccgcagc
     5461 ggcccggtgg gcacgccgtg gtcgaaggtc atccggaagg cgtggccctt ctggcgaaca
     5521 cacacatcga ggcgggagga gagggcgttg acgacggagg agccgacgcc gtgcagaccg
     5581 ccggagacct tgtagccgcc gccgccgaac ttgccgccgg cgtgcagct
//
LOCUS       DAOPJG010000209         5589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_15623_length_5589_cov_5.326708, whole genome shotgun sequence.
ACCESSION   DAOPJG010000209 DAOPJG010000000
VERSION     DAOPJG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15623_length_5589_cov_5.326708"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..622
                     /locus_tag="PKU75_14715"
     CDS             65..622
                     /locus_tag="PKU75_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPF82177.1"
                     /translation="MPQVSADVYVPVPPELAFAVSQTTGQVRLRWDPFIRRQFFLDGA
                     TAPGPGVRTKTFARLGPSMVSEYASYRPPTSVGMTMVRGPWFFSRFGGGWRFEREGTG
                     CRAVWKYTFDVRPDWLRPVGHRLGHWLLGREIRRRIHGFAKGCTDPVVVEAARALLAR
                     VDDGALDEEARREQRTLGHDLRYQQ"
     gene            complement(560..1546)
                     /locus_tag="PKU75_14720"
                     /pseudo
     CDS             complement(560..1546)
                     /locus_tag="PKU75_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(1553..2560)
                     /locus_tag="PKU75_14725"
     CDS             complement(1553..2560)
                     /locus_tag="PKU75_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPF82178.1"
                     /translation="MTTTVNSLRQDLDPAPETPQGPTPPKRKVGRRAVLGGGFVALFG
                     GIGATGGWALNRYVVDHVEVENVSQQTSTAIEAVQADTASATSTATSYTSDTAQISIE
                     KVVTGSGSDQVTYFVADVTISDATILKSAFANDQFGNNIIAYPSAIAAQANAVLAING
                     DYYGFRDNGIQIRNGVIYRDNGARQGLAIYKDGSMKLYDETATNAKTLLSEGVWQTLS
                     FGPGLVEGGKILDGIDNVEVDTNFGNHSIQGQQPRTGIGMIADNHFLFVAVDGRSSGY
                     SRGVTMNEMAQIFVDKGAQVAYNLDGGGSTAMIFQGDLVNNPLGKGQERGTSDIVYIA
                     G"
     gene            complement(2682..4286)
                     /locus_tag="PKU75_14730"
     CDS             complement(2682..4286)
                     /locus_tag="PKU75_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPF82179.1"
                     /translation="MQRNRLTTTAVALTAALGLAACGAGNDASTATAGAASTPAATAA
                     ASSGSTPAAAASSTSNASGASTSSALLADNAKAHAEEGDPAYDAEDVVKVTLQDGASS
                     ASTQTGVQIDGDTVTISAPGTYVLSGTLTDGTIVVNSADEGRVRLVLDNAAVTNSQGA
                     ALNIVAADEAVVVLEEGTTNSLADGTGYDTSAEDAPNAALFSMADLTIGGSGTLKVTG
                     NTNDGIASKDGLVILGGTVDVTAADDGIRGKDYLVVQGGTVTVTAGDDGLKSDNEDDD
                     TVGYILLAGGTTTVDAGDDGAHAEGDLAVTGGSVTITKSNEGLEGANVVIAGGTSSVT
                     ASDDGLNATQGSSSGGGGMGGGGMANDGSQLVISGGTLVVDSGGDGLDSNGQTTISGG
                     TTVVYGPTMNGNGSLDSNGGIDVTGGTVLAAGSGGMAEAPDTSSTQGWVQLGFSSNVT
                     AGQTVQIVSGDTVVASFTAKKDYSNLVFVGDDITSGQSYDVYVGSGSLGSDQLATFSL
                     GGSTDGATKVGSVTAGQATGGMGGPR"
     gene            complement(4360..5181)
                     /locus_tag="PKU75_14735"
     CDS             complement(4360..5181)
                     /locus_tag="PKU75_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPF82180.1"
                     /translation="MTTVTVAAYQPFQRMTDLRDLDPISLPELIGAAEMLTRVDRKYV
                     VPRAALGALAGRLATTQAGKRTRALEIDGSREFGYRSTYWDTADLASFRGAGQKRRRR
                     FKVRTRTYLDSGASYLEVKTRGPRGTTVKNRIEHPEILDTRLDPVAVAYVDGLLTDAL
                     VPDVAAAELVPTLITRYRRTTLATTAPGVPATRATIDTDLEWHAPGGTHLTLPDLVIV
                     ETKGTTSPSVVDRALWRMGHRPVRVSKYGTGLRAVRPELPDLKWHRVLHEHFLAA"
     gene            complement(5178..>5589)
                     /locus_tag="PKU75_14740"
     CDS             complement(5178..>5589)
                     /locus_tag="PKU75_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPF82181.1"
                     /translation="GLGLFGVLSIIRLRSEELSNTEVAYYFAALALGLLGGLGSTNVP
                     LVAALMGLLVAVFLIGDHPAVSRKTERQTMVLDRAYGDVTAARAHVERVLGTEVSTVS
                     VVKTDLVNDTTTVEVRYTPATGARALAPAAEVTR"
BASE COUNT          785 a         1782 c         2079 g          943 t
ORIGIN      
        1 cccctccgcg cacctcacgt catcaaaccg ctgccccgtg aaagacggag gctagggttg
       61 tggtatgccg caggtcagtg ccgacgtcta cgtccccgtg cccccggagc tcgccttcgc
      121 cgtttcgcag acgaccggcc aggtccggtt gcggtgggac ccgttcatcc ggcgccagtt
      181 cttcctggac ggtgccactg cgccgggccc aggggtgcgc accaagacct ttgcgcggct
      241 ggggccgagc atggtcagcg agtacgcgtc ataccgtccg ccgacgtcgg tcgggatgac
      301 gatggtgcgc ggaccgtggt tcttttcgcg attcggcggc ggctggcgct tcgagcggga
      361 gggcacgggc tgccgggccg tctggaagta caccttcgac gtgcggccgg attggctgcg
      421 cccggtgggg catcggctcg gtcactggct acttggccgt gagatccgcc gacgcatcca
      481 cggattcgcg aaaggatgca ccgatccggt cgtcgtggag gctgcccgcg cgcttcttgc
      541 acgagtagac gacggcgcgc tggatgagga agctcgccgc gaacagcgta ccctcggtca
      601 cgaccttcgc taccagcagt gatccggttg cggccagaag tgcatgcagc agaagaatat
      661 tcgccacaag tatggtcgct gcgagcgcgg cataccgcag gagcgagggg agcagcgggg
      721 ccttggcgga tccgttgcgg aagacccacc accggttgac cgcatagttg ctgccgccgc
      781 tgaccacccg ggcggcgatg gccgatcccg tgacatttcc ggtcagcgcg acgcccgcga
      841 agagcacgga ggtgtccagc acgaaggcga gcagcgagga tccaacgaag gagatcagct
      901 gccgatagat gagcgccgag tcgtgcagcg gacggaagtg ggacgaggcg ttctcgtcga
      961 ggtagatcgt cgcgatctcg acctcacgaa tgtcctgatc ctccgcggtc gcgcgcagca
     1021 ggagttgcag ctcgtattcg aagcggtcgc cgggcacctc cagcagccac gggatcaacc
     1081 gggccgggta ggcccgcagg ccggtctgag tgtcgatcag cttgcgacgg gtcgccaggg
     1141 cgaacaactg ccgggtgatg ccattgccga ccttgctgcg cagcgggacc cgaccggtga
     1201 agagccgggc gccgaggacc atgtcggggc cgctgtcgtc gagggcggcg gcaacgcgca
     1261 ggatgtcgac gggcgaatgc tggccgtcgc tgtcggcgca gacgaccggt tcaccgggga
     1321 aaacctgtgc gaggtaggcg aatccggtct tgagggcagc acccttgcca cggttgacct
     1381 ggtggcgcag cacggtcgcg ccgcgccgct cgcactcggc gaagatcgcc gcatagtccg
     1441 gcccgctgcc gtcgtcgacg atgacgaccg gcgaggcgcc gccgaggtcg gcgaccagct
     1501 gggggaggcg ctggtccggc tcgtaggacg ggatgagaac gatcatggcc aatcacccgg
     1561 cgatgtagac gatgtcgctc gtcccgcgct cttggccctt gccgagcggg ttgttgacga
     1621 gatcgccctg gaagatcatc gccgtggagc caccgccgtc gaggttgtag gcgacctggg
     1681 cacccttgtc gacgaagatc tgcgccatct cgttcatcgt gacgccgcgg ctgtagccgg
     1741 aactgcggcc gtccacggcg acgaagagga agtggttgtc ggcgatcatc ccgatgcccg
     1801 tgcgcggttg ctggccttgg atcgagtggt tgccgaagtt ggtgtcgacc tcgacattgt
     1861 cgatgccgtc gaggatcttg ccgccctcga ccaggcccgg gccgaacgac agggtctgcc
     1921 agaccccttc ggagagaagg gtcttggcat tggtggcggt ctcgtcatag agcttcatcg
     1981 agccgtcctt gtagatcgcc aggccctgcc gcgcaccatt gtcgcgatag atcacgccat
     2041 tgcggatctg gatgccgttg tcgcggaagc cgtagtagtc gccgttgatc gcgaggacgg
     2101 cgtttgcctg ggcggcgatc gccgacgggt aggcaatgat gttgttgccg aattggtcgt
     2161 tggcgaaggc cgacttgagg atcgttgcgt cgctgatggt gacgtcggcg acgaagtagg
     2221 tgacctggtc gctcccgctg ccggtgacga ccttctcgat cgagatctgc gccgtgtcgc
     2281 tcgtgtaact cgtggcggtg ctcgtggcgc tcgcggtgtc ggcctgcacg gcctcgatgg
     2341 ccgtggacgt ctgctgcgag acgttctcca cctcgacgtg gtccacgacg tagcggttga
     2401 gcgcccagcc accggtggca ccgatgccgc cgaacagcgc gacgaagcca ccgccgagga
     2461 cggcgcgccg gcctaccttc cgcttcgggg gtgtggggcc ctgcggggtt tccggggccg
     2521 ggtcgaggtc ctgccggagg gagttcacgg tggtggtcat gtcacggtcc ttatcgggtt
     2581 gaagcgcaat gggtttgggg caaggggcaa agccgggttc gcccggccgg ccggagccag
     2641 ggtggtcggc cgggctgctt cttggttggg cgcgggggtg gtcatctggg gccacccatg
     2701 ccgcccgtcg cctggcccgc agtgacggag ccgaccttgg tggcgccgtc ggtggagccg
     2761 ccgagggaga aggtggcgag ctggtcggac ccgagagagc cggagccgac gtagacgtca
     2821 tacgactgtc cgctggtgat gtcgtcaccg acgaacacga gattgctgta gtccttcttc
     2881 gcggtgaagc tggcgaccac cgtgtcaccg gagacgatct gaaccgtctg gcccgcagtg
     2941 acattcgagg agaagcccag ctgcacccag ccctgggtgg agctggtgtc gggtgcctcg
     3001 gccatgcccc cggagcctgc ggccagcacg gtgccgcccg tgacgtcgat gccgccgttg
     3061 gagtcgaggg agccgttgcc gttcatcgtc ggcccgtaga cgacggtcgt gccgccgctg
     3121 atcgtggtct ggccgttgga gtccaggccg tcgccgccgc tgtcgacgac gagggtgccg
     3181 ccggagatga ccagctgtga gccgtcgttc gccattccgc cgccgcccat gccgccgcca
     3241 ccggagctcg acccctgcgt ggcattgagg ccgtcgtcgc tcgcggtgac cgaggaggtg
     3301 ccgcccgcga tcacgacatt ggcgccttcg aggccctcgt tcgacttcgt gatcgtcacg
     3361 gacccgccgg tcaccgcgag gtcgccctcg gcgtgagcgc cgtcgtcgcc ggcgtcgacg
     3421 gtggtcgtgc cgccggccag gaggatgtag ccgaccgtgt cgtcgtcctc gttgtccgac
     3481 ttcaggccgt cgtcgccggc ggtgaccgtc acggtcccgc cctggacgac gaggtagtcc
     3541 ttgccgcgga tgccgtcgtc ggccgcggtg acgtcgacgg tgccgccgag gatgaccagg
     3601 ccgtccttgg aggcgatgcc gtcgttggtg ttgccggtca ccttcagggt gccactccca
     3661 ccgatcgtca ggtcggccat cgagaagagg gcggcgttgg gagcgtcctc ggcggacgtg
     3721 tcgtagcccg tgccgtcggc gaggctgttg gttgtgccct cctcgaggac cacgaccgct
     3781 tcgtcggccg ccacgatatt gagggcggcg ccctggctgt tggtgacggc ggcattgtcc
     3841 agcaccagcc ggaccctgcc ctcgtccgcg ctgttcacga cgatggtgcc atcggtcagc
     3901 gtcccggaca ggacataggt gccgggtgcg ctgatcgtca ccgtgtcccc gtcgatctgc
     3961 acgccggtct gggtcgaggc gctgctcgcc ccgtcctgca gcgtgacctt cacgacgtcc
     4021 tccgcgtcgt atgccgggtc gccctcttcc gcatgcgcct tggcgttgtc agcgagcagt
     4081 gcggagctgg tggaggcgcc ggaggcgttg gaggtgctgg aggcggcggc cgcgggcgtc
     4141 gacccgctgc tggcggcggc ggtcgcggcc ggggtggagg ccgcgccggc ggtcgccgtc
     4201 gacgcgtcgt tgccggcgcc gcaggccgcg aggccgaggg cggccgtcaa ggcgacggcg
     4261 gtggtggtga ggcggttgcg ctgcatgggg gtggctcctg ggactgctcg taaagggatt
     4321 gggggagaag gatttccggc ggtcttcccg gtggggtgat caggcggcga ggaagtgctc
     4381 gtgcaggacg cggtgccact tcaggtcggg cagctcgggt cggacggccc gcaggccggt
     4441 gccgtacttg ctcacgcgca ccgggcggtg ccccatccgc cagagcgccc ggtcgacgac
     4501 cgacggagag gtggtgccct tggtctcgac gatgacgagg tccggcaacg tcaggtgcgt
     4561 gccaccgggc gcgtgccact cgaggtcggt gtcgatggtg gcccgggtgg ccggcacccc
     4621 cggcgctgtc gtggccagcg tcgtgcggcg gtagcgggtg atcagcgtcg ggaccagctc
     4681 cgcggcagca acatcgggca cgagcgcgtc ggtgagcagg ccgtcgacat aggccaccgc
     4741 cacggggtcg agccgggtgt cgaggatctc ggggtgttcg atgcggttct tgacagtggt
     4801 gccgcgcggg ccgcgggtct tgacctccag gtaactcgcg ccgctgtcca gataggtccg
     4861 cgtccgcacc ttgaaccgcc gccgccgttt ctggcccgca ccccggaagc tcgccaggtc
     4921 ggcggtgtcc cagtaggtcg atcggtagcc gaactcgcgg ctcccgtcga tctccagggc
     4981 ccgggtgcgc ttgccggcct gggtggtggc cagccggccc gccagcgcgc cgagggcggc
     5041 gcgcgggacg acatacttcc ggtccacccg ggtgagcatc tcggcggcgc cgatcagctc
     5101 cgggagcgag atcgggtcga ggtcacgcag atcggtcatg cgctgaaagg gctggtatgc
     5161 cgcgacggtg acggtggtca tcgggtcacc tcggccgcgg gggcgagcgc ccgggcgccg
     5221 gtcgccggcg tgtagcggac ctcgacggtc gtggtgtcgt tgacgaggtc ggtcttgacg
     5281 acgctgaccg tggacacctc cgtgccgagc acgcgttcga cgtgggcgcg ggcggccgtc
     5341 acgtcgccgt atgcccggtc gagcaccatg gtctggcgct cggtcttgcg cgagacggcc
     5401 gggtggtcgc cgatgaggaa gacggcgacg agcaagccca tcagggccgc gacgagcggg
     5461 acgttcgtgc tacccagccc gccgagcagt ccgagggcga gggcggcgaa gtagtaggcc
     5521 acctcggtgt tgctcagttc ctccgagcgc agccggatga tcgacaagac cccgaagagc
     5581 cccagaccc
//
LOCUS       DAOPJG010000210         5587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_15632_length_5587_cov_7.056399, whole genome shotgun sequence.
ACCESSION   DAOPJG010000210 DAOPJG010000000
VERSION     DAOPJG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15632_length_5587_cov_7.056399"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="rplB"
                     /locus_tag="PKU75_14745"
     CDS             complement(<1..706)
                     /gene="rplB"
                     /locus_tag="PKU75_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPF82182.1"
                     /translation="MGIRKYKPTTPGRRGSSVADFVEVTRSTPEKSLVKPLSKSGGRN
                     ASGRITTRHIGGGHKRAYRVIDFRRHDKDGVPAKVAHIEYDPNRTARIALLHYADGEK
                     RYILAPKGLEQGMAIENGPGADIKPGNSLPLRNIPTGTVVHAVELKPGGGAKIARSAG
                     VRVQLVAKDGPFAQLRMPSGEIRNVDLRCRATIGEVGNAEQSNINWGKAGRMRWKGKR
                     PTVRGVAMNPVDHPHGG"
     gene            complement(723..1022)
                     /gene="rplW"
                     /locus_tag="PKU75_14750"
     CDS             complement(723..1022)
                     /gene="rplW"
                     /locus_tag="PKU75_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPF82183.1"
                     /translation="MSTVHKDPRDILIAPVVSEKSYGLLEEGKYTFIVDPRSNKTEIK
                     IAVEQIFGVKVDSVHTMNRPGKARRTRFGMGKRKDTKRAIVTLREGTIDIFGAGA"
     gene            complement(1019..1633)
                     /gene="rplD"
                     /locus_tag="PKU75_14755"
     CDS             complement(1019..1633)
                     /gene="rplD"
                     /locus_tag="PKU75_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPF82184.1"
                     /translation="MTAKTVTVDLPAEIFDVQTNVPLIHQVVVAQLAAARQGTHATKT
                     RGEVRGGGRKPYRQKGTGRARQGSTRAPQFAGGGTVHGPQPRDYSQRTPKKMKAAALR
                     GALSDRARHDRIHVVEALVTGETPSTKQAAELLAGLTERKNLLVVLDRTDLVSLKSVR
                     NLDNVHVLAVDQLNTYDVLCADDVVFTKAAFDTFVGTGAQEDTK"
     gene            complement(1636..2292)
                     /gene="rplC"
                     /locus_tag="PKU75_14760"
     CDS             complement(1636..2292)
                     /gene="rplC"
                     /locus_tag="PKU75_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPF82185.1"
                     /translation="MTHATTRSVKGVLGEKLGMTQVWDADSRLIPVTVVKAGPCVVTQ
                     VRGAETDGYDAVQIAYGEINPRKVNQPMGGHFDKAGVTPRRHVIELRTSDAADYTVGS
                     EVTAEVFEAGQKVDVSGTTKGKGFAGVMKRHGFKGVSASHGAHRNHRKPGSIGGCSTP
                     GRVFKGMRMAGRMGGVRQTTQNLTVHAVDADKGLLLIKGAVPGPRGGLVLVRSAAKGA
                     "
     gene            complement(2302..2610)
                     /gene="rpsJ"
                     /locus_tag="PKU75_14765"
     CDS             complement(2302..2610)
                     /gene="rpsJ"
                     /locus_tag="PKU75_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPF82186.1"
                     /translation="MAGQKIRIRLKSYDHEVIDSSARKIVDTVTRAGATVVGPVPLPT
                     EKNVFVVIRSPHKYKDSREHFEMRTHKRLIDIIDPTPKAVDSLMRLDLPADVNIEIKL
                     "
     gene            3033..3554
                     /locus_tag="PKU75_14770"
     CDS             3033..3554
                     /locus_tag="PKU75_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPF82187.1"
                     /translation="MDARDVLIDAAGRPVDSARQVLDGLDGDTLHRMPDDRHSSIAWL
                     LWHAARQADVQVAELAGTQEVWRAAGFIGRFALDRPDDAMGFGDSPEDVAKVRVDDPG
                     LLTAYLEAVFEATTTYVRGLTEADLDDIVDRRWDPPVTRGVRLVSVIDDAVAHIAQAQ
                     YLRGLLADWSIGY"
     gene            3556..4074
                     /locus_tag="PKU75_14775"
     CDS             3556..4074
                     /locus_tag="PKU75_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF82188.1"
                     /translation="MSPRHTPRGKPTLTTERLTLRPLTWDDLPELEALDADPVVMEFL
                     DRPRTADEVRERTLARLDPTHDAMGLGYWCGLERSRFVGWWLLTPEGPGLAEIGWRLH
                     PWAWGRGLATEGAREIIRHGFETVGLERIVAETMVVNARSRSVMDKLGMRHTRVEVRP
                     WEDPLPGAEQGE"
     gene            complement(4106..4732)
                     /locus_tag="PKU75_14780"
     CDS             complement(4106..4732)
                     /locus_tag="PKU75_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82189.1"
                     /translation="MTIVTRRCGARMAVVSVFAAVTAAGPVADAAVATWREIPSPDLT
                     ANARYEDVAAVNSTLAWAVGSEGETGAPREVLIRWNGTAWRRTDLPGVIRLTGVSADA
                     TNRAWIVGQAGPGDPWGGSPYAGRLTASGWAPATNGIAPGAVPTAVATRGATTILVGY
                     RPAVYPARAPELDDLAAVRGSNRVWGGGSQPRLHGSLLKNFALVAVNR"
     gene            complement(5002..>5587)
                     /locus_tag="PKU75_14785"
     CDS             complement(5002..>5587)
                     /locus_tag="PKU75_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPF82190.1"
                     /translation="PERETDKPFLMPVEDVFTITGRGTVVTGRVERGILKVNEEVEIV
                     GIHEGPATKTTVTGVEMFRKLLDEARAGENVGLLLRGIKREDVERGQVVVKPGSITPH
                     TDFEANVYILGKEEGGRHTPFYDNYRPQFYFRTTDVTGVVKLPEGKEMVMPGDNTEMN
                     VELIQPIAMEEGLKFAIREGGRTVGAGRVTKINK"
BASE COUNT          772 a         1843 c         2017 g          955 t
ORIGIN      
        1 caccaccgtg cgggtggtcg accgggttca tcgcgacacc gcggacggtc gggcgcttgc
       61 ccttccaccg catacggccg gccttgcccc agttgatgtt ggactgctcg gcgttgccga
      121 cctcaccgat ggtggcgcgg cagcgcaggt ccacattgcg gatctcaccg gacggcatac
      181 gcagctgggc gaaggggccg tccttggcga cgagctggac gcggacgccg gcagagcggg
      241 cgatcttggc gcccccaccg ggcttgagct cgaccgcgtg cacgaccgta ccggtcggga
      301 tattgcgcaa cggcaggctg ttgcccggct tgatgtcggc acccggaccg ttctcgatcg
      361 ccatgccctg ctccaggccc ttgggggcga ggatgtagcg cttctcgccg tcggcgtagt
      421 gcagcagcgc gatgcgagcg gtgcggttgg ggtcgtactc gatgtgcgcg accttggccg
      481 gcacgccgtc cttgtcgtgg cgacggaagt cgatcacgcg gtaggcgcgc ttgtggccac
      541 caccgatgtg ccgggtggtg atgcgcccgg aggcgttgcg cccaccggac ttggagagcg
      601 gcttgaccag cgacttctcc ggcgtgctgc gagtgacctc gacgaagtcg gcgacgctgc
      661 tgccacgacg acccggggtc gtcggcttgt atttgcggat tcccatgtct acctaagctc
      721 ctttaggcgc ccgcaccgaa gatgtcgatc gtgccctcgc ggagggtgac gatcgcacgc
      781 ttggtgtcct tgcgcttgcc catgccgaag cgggtgcggc gggccttgcc cgggcggttc
      841 atcgtgtgga ccgagtcgac cttgacgccg aagatctgct cgacggcgat cttgatctcg
      901 gtcttgttcg agcgcgggtc gacgatgaag gtgtacttgc cctcctcgag gaggccatag
      961 ctcttctcgg agacgaccgg cgcgatcagg atgtcgcggg ggtccttgtg cacggtgctc
     1021 acttggtgtc ctcctgggcg ccggtcccga cgaaggtgtc gaaggcggcc ttggtgaaga
     1081 ccacgtcgtc ggcgcacagc acgtcatagg tgttgagctg gtcgaccgcg aggacgtgga
     1141 cgttgtcgag gttgcgcacc gacttcagcg acaccaggtc ggtgcggtcg agcacgacca
     1201 gcaggttctt gcgctcggtc aggccggcga gcagctcggc ggcctgcttg gtcgaggggg
     1261 tctcgccggt gacgagggcc tcgacgacgt ggatgcggtc gtggcgggcc cggtcggaga
     1321 gggcaccgcg cagggcggcg gccttcatct tcttgggggt gcgctggctg tagtcacgcg
     1381 gctgcgggcc gtggaccgtg ccaccgccgg cgaactgcgg cgcgcgggtc gaaccctggc
     1441 gggcgcggcc ggtgcccttc tgacggtagg gcttgcggcc accaccgcgg acttcgccgc
     1501 gggtcttggt ggcgtgcgtg ccctgacggg cggcggccag ctgcgcgacg acgacctggt
     1561 ggatcagcgg gacgttggtc tggacgtcga agatctcagc gggaaggtcg accgtgacgg
     1621 tcttggcggt catgttcagg ctcccttcgc ggcggagcgg acgaggacga ggccaccgcg
     1681 ggggccgggg acggcgccct tgatgagcag caggcccttg tcggcgtcga cggcgtgcac
     1741 ggtgaggttc tgggtggtct ggcgcacccc gcccatccgg ccggccatac gcatgccctt
     1801 gaagacgcgg cccggggtgg agcacccgcc gatcgaaccc ggcttgcggt ggttgcggtg
     1861 cgcaccgtgg gaggcggaga cacccttgaa gccgtggcgc ttcatgacgc cggcgaagcc
     1921 cttgcccttg gtggtgcccg agacgtcgac cttctggccg gcctcgaaga cctcggcggt
     1981 gacctcggag ccgacggtgt agtcggccgc gtcggaggtg cgcagctcga tgacgtggcg
     2041 gcgcggggtc acgccggcct tgtcgaagtg gccccccatg ggctggttga ccttgcgcgg
     2101 gttgatctcg ccgtaggcga tctggactgc gtcgtagccg tcggtctcgg cgccgcggac
     2161 ctgcgtgacg acgcagggcc cggccttgac gacggtcacc gggatgagcc ggctgtcggc
     2221 gtcccagacc tgggtcatgc cgagcttctc gccgagcaca cccttgacgg agcgcgtggt
     2281 tgcgtgagtc atggcgtcct cttagagctt gatctcgatg ttgacgtccg ccggcaggtc
     2341 gagacgcatc agcgagtcga cggccttggg cgtcgggtcg atgatgtcga tgaggcgctt
     2401 gtgcgtgcgc atctcgaagt gctcgcggga gtccttgtac ttgtgggggc tccggatgac
     2461 cacgaagacg ttcttctccg tgggcagggg caccgggccg acaacggtgg cgcccgcgcg
     2521 ggtgaccgtg tcgacgatct tgcgcgccga gctgtcgatg acctcgtggt catacgactt
     2581 cagccggatg cggatcttct gtcccgccat cttctggtcg tctctctctc gccgtccaac
     2641 taacaaatcc gtgagggccc cgtctggcct ccgacccccg cggtcgggcg tgtcgcccta
     2701 gctccgctcc cgaaggcgcg cagttccctg cgatccttcg ggggaatcga atggcccggc
     2761 cggagccgaa cgcttgcgcg gcgccggtgt gtcgccccgc tcccagagcg gttcgattcg
     2821 ttcgcacctg ctgcggcggg cgacccacag gatcgacgcc tcgcgtcaag caacctgact
     2881 atcttgccat gccgcgcgag ccaggccaaa tccggagccc ttcgcacttt tccgcccagt
     2941 gccacttggt atcggtccgc cgcgggaagt tcgcgatgcg caccgcagga aacctgccca
     3001 aaggctcgcc tcggggtggc aagctcgtcg gtatggatgc tcgtgatgtg ctgatcgacg
     3061 ccgccggtcg gccggtggac tccgctcgcc aggtcctgga cggcctggac ggcgacacgc
     3121 tccatcggat gcccgacgac cggcattcct cgatcgcctg gctgctctgg cacgccgcgc
     3181 ggcaggcgga cgtgcaggtc gccgagctgg ccgggacgca ggaggtatgg cgtgcggccg
     3241 gcttcatcgg ccggttcgcc ctcgaccggc cggacgacgc gatggggttc ggcgactccc
     3301 ctgaggacgt ggcgaaggtg cgagtcgacg acccggggtt gctgacggca tacctggaag
     3361 cggtgttcga ggcgaccacg acatatgtcc gcggcctcac tgaggccgac ctcgacgaca
     3421 tcgtcgaccg gcgctgggac ccgcccgtga cccgcggggt gcggctggtc agcgtcatcg
     3481 acgacgccgt ggcgcacatc gcgcaggcgc aatacctgcg cgggctgctc gcggactggt
     3541 cgatcggtta ctgaggtgag tccgcggcat acgccgcgcg ggaaacccac cctgaccacc
     3601 gagcggctca ccctgcggcc gctgacgtgg gacgacctgc cggagctgga agcgctcgac
     3661 gccgatccgg tggtgatgga attcctcgac cggccgcgca cggcggacga ggtccgcgag
     3721 cgcacccttg cccgactgga cccgacgcac gacgcgatgg gcctcggcta ctggtgcggc
     3781 ctcgagcgca gccgcttcgt cgggtggtgg ctcctgaccc ccgaaggtcc cgggctggcc
     3841 gagatcggct ggcgtctcca cccgtgggcg tggggccgcg gcctggcgac cgagggcgcc
     3901 cgagagatca tccggcacgg cttcgagacc gtcggtctgg agcgcatcgt cgccgagacg
     3961 atggtggtca acgcgcgcag ccgctcggtg atggacaagc tgggtatgcg gcatacccgc
     4021 gtggaggtgc ggccctggga ggacccgctc cccggcgccg agcagggcga gtaactcgct
     4081 gagatcaccc gcgccgactg gatcgctagc ggttgaccgc gaccagggcg aagttcttca
     4141 gcaggctccc gtgcagacgc ggttgagagc cccctcccca gacgcggttc gaaccgcgga
     4201 ccgcggcgag gtcatctagt tccggcgccc gcgcgggata gacggccggg cggtagccca
     4261 ccaggatggt ggtcgctccg cgggtggcaa ctgcggtggg gactgctcct ggggcgatgc
     4321 cgttagtcgc gggggcccat cctgaggcgg tgaggcggcc ggcatacggg ctcccgcccc
     4381 agggatctcc ggggccggcc tgtccgacga tccacgcccg attcgtggcg tccgcgctga
     4441 cgcccgtcaa ccggatgacg cctggcaggt cggtgcgccg ccaggcagtg ccattccagc
     4501 ggatgagcac ctcccgtggg gccccggtct cgccttccga gccaacggcc cacgcgagcg
     4561 tcgagttcac cgcggccacg tcctcatatc gcgcattcgc cgtcaagtcc ggcgacggga
     4621 tctcccgcca ggtcgccacg gcggcgtccg caaccggccc ggccgcggtc acggcggcga
     4681 acacgctcac caccgccatc cgggcgccgc accgacgagt cacgatagtc atgaaccgca
     4741 gtgcagcgtg cccgccccgg ccaagggcaa gggcttgacg ttggccgtaa gaacttcgtc
     4801 agtctggctg gcctctcggc agccgccccg ggcacagaca cggtgtggga tcgctggggg
     4861 cgtggggcac tcggcgcccc gaaacagaag agcgcggcac ccgcgaaacg ggtaccgcgc
     4921 tcttctgttc tttcacgggg cttaccccgt gtgggggccg ctcctcgtgg ggggtccacg
     4981 gggagcgaag ccccccgtgt gttacttgtt gatcttcgtc acgcgaccgg cgccgacggt
     5041 gcggccgcct tcacggatgg cgaacttcag gccctcttcc atggcgatgg gctggatgag
     5101 ctcgacgttc atctcggtgt tgtcgccggg catgaccatt tccttgccct cggggagctt
     5161 gacgacaccc gtcacgtccg tggtgcggaa gtagaactgc ggacggtagt tgtcgtagaa
     5221 cggcgtgtga cggccgccct cctccttgcc gaggatgtag acgttggcct cgaagtcggt
     5281 gtgcggggtg atcgaacccg gcttgacgac gacctggccg cgctcgacgt cctcgcgctt
     5341 gatgccacga agcagcagac cgacgttctc gccggcgcgg gcctcgtcga gcagcttgcg
     5401 gaacatctcg acaccggtga cggtggtctt ggtggccggg ccctcgtgga tgccgacgat
     5461 ctcgacctcc tcgttgacct tgaggatgcc acgctcgaca cggccggtga cgacggtgcc
     5521 acgaccggtg atggtgaaga cgtcctcgac gggcatgagg aacggcttgt ccgtctcacg
     5581 ctcgggc
//
LOCUS       DAOPJG010000211         5543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_15805_length_5543_cov_8.286990, whole genome shotgun sequence.
ACCESSION   DAOPJG010000211 DAOPJG010000000
VERSION     DAOPJG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15805_length_5543_cov_8.286990"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..651
                     /gene="rplU"
                     /locus_tag="PKU75_14790"
     CDS             343..651
                     /gene="rplU"
                     /locus_tag="PKU75_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPF82191.1"
                     /translation="MYAIVKAGGRQEKVAVGDVLLIDKVSGKAGDKLDLTPLLLIDGT
                     TVTSDASKLAKVSVQAEVVGPAKGPKITIMKFKNKTGYRKRQGHRQHLTQVKITAIDA
                     "
     gene            679..933
                     /gene="rpmA"
                     /locus_tag="PKU75_14795"
     CDS             679..933
                     /gene="rpmA"
                     /locus_tag="PKU75_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPF82192.1"
                     /translation="MAHKKGASSTRNGRDSNAQRLGVKRYGGQVVGTGEIIVRQRGTH
                     FHPGDNVGRGGDDTLFALADGKVEFGTKRGRRVVNVVAGE"
     gene            1056..2573
                     /gene="obgE"
                     /locus_tag="PKU75_14800"
     CDS             1056..2573
                     /gene="obgE"
                     /locus_tag="PKU75_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPF82193.1"
                     /translation="MTTFVDRVTLHVTAGDGGHGVASIRREKFKPLGGPDGGNGGNGG
                     NVVITVDPQSTTLLDYHRNPHRRAENGRPGAGDERDGAKGGDLILPVPEGTVVSTRDG
                     EVIADLVGPGASVVIAEGGRGGLGNRALSSTKRKAPGFALLGEPGESVDIVLELKSLA
                     DVALIGFPSAGKSSLVSVISAARPKIADYPFTTLVPNLGVVTAGSMRFTVADVPGLIP
                     GASEGRGLGLEFLRHVERCSVLVHVIDCATLEPGRDPLSDLDAIEAELAAYVPGDLLG
                     GRPLAERVRMVVLNKADVPEARELAEMVEPDLRERGYEVFIVSAVAHVGLKEWIFAMA
                     RAVEAARAEQESEIVPQRVTIRPKAVDDKGFQVVREQDGGETVFRVVGDKTTRWVRQT
                     DFSNDEAVGYLADRLQRAGVEDALLAAGAVPGATVLIGPADNAVVFDWDPTMVGGAEL
                     LGGRGTDGRFDDRSRRTNTERREEFHARKDAQTAAREELAAERKAGHWATPDTDQ"
     gene            2612..3760
                     /gene="proB"
                     /locus_tag="PKU75_14805"
     CDS             2612..3760
                     /gene="proB"
                     /locus_tag="PKU75_14805"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPF82194.1"
                     /translation="MSTDELAPAELAAARARIAGRGRIVVKVGSSSLTTDGGRRLDPA
                     RIGALVAPLADRRLRGEPIVLVSSGAIAAGIGPLGLARRPKDLATQQAAASVGQGALV
                     AAYAQAFAAYGLTVGQVLLTADDVTRRSHYTNARRTLDRLLELGIVPIINENDTVATH
                     EIRFGDNDRLAALVSHLVKAEALVLLTDVDGLYDGPPSEPGTQRISLIRSLADLETVR
                     IGGTGSMVGTGGMATKIEAAAIANSAGITAVLCAAPAVGEALAGADVGTVFLPHSVGK
                     ASRKLWIAHATTPRGVLTVDAGAVAALVERRKSLLPAGIIAVTGAFADGDPVDVAGPD
                     GVVIARGLVSYDAGELPRMMGRSTRELARDLGPEYEREVIHRDNLVVR"
     gene            complement(3864..4769)
                     /locus_tag="PKU75_14810"
     CDS             complement(3864..4769)
                     /locus_tag="PKU75_14810"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF82195.1"
                     /translation="MSAAAEFKASVVEMKTYPVEGTAIEVKKLYEGTYGEVPETLNGW
                     FHRDDWPRTAYVLSRMDDGERALDVGVGAGQFLNMVALSGKYSGVYGADPTRFGKYIE
                     YSDLIHRTNDGIDKLPYPDDHFDVVTCMEVLEHIPEEIFTAGLAELRRVCRGQLLMTV
                     PFEEPEPISKTHVRRFERQDVVDNFPDGRYTILNRWRMPWVLIEERMDGKPFAPLDEP
                     MFDDTPKPKTAAAKPAATASKATASKATGSKATGSKLTVEQRRIEQLEATVARLKSRR
                     SVRTADWAGAQARRVKQAAKRRLGK"
     gene            4934..>5543
                     /locus_tag="PKU75_14815"
     CDS             4934..>5543
                     /locus_tag="PKU75_14815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF82196.1"
                     /translation="MTLISSRPVTRSASILSPEHRWVTIAAIALVTLGAYENRAITTI
                     LPVIADDLDGLRWFGLASAIPAATYLVATTVAGTWTDHRGPRRPLLFSLVLFALAQIA
                     SGLAPSMEVLLAARALSGIAEGGLDIALVVLVSDLMPEALRAKVFAAFATAWIVPSLV
                     GPAVAGGIAQWLGWRAAFLLPLIMLIAVVPALLPALRRATARG"
BASE COUNT          834 a         2009 c         1872 g          828 t
ORIGIN      
        1 ctgctcaagc tgccgacgtc agggaggacg ccgaggagga gacgaaggac gaggtcatgg
       61 acgaggccac ggcccagccg aagcgtggaa cccgccgggg cggccgccgc cgcggccggg
      121 ccaccgcccc cgccggcccg ccgcgcccgg caacggacgc ctgacgaggt cgcggccaag
      181 gggagacaag ggccgagccg cggaagcgcg tttggtcgca acacctgcca ggccttagca
      241 ttgatccttg gtgcgcccac cgcgtcgatg tcccgatccc ggggtagcta ttggccggtc
      301 atccgagagt gcgcgcaaga cgtaagcgaa agtgagatca acgtgtacgc gatcgttaaa
      361 gcgggcggtc gtcaggagaa ggtcgccgtc ggcgacgtcc tcctgatcga caaggtgtcc
      421 ggcaaggccg gcgacaagct ggacctgacc cctcttctgc tgatcgacgg caccaccgtc
      481 acctctgatg ccagcaagct ggccaaggtg tcggtccagg ccgaggtcgt cggccccgcc
      541 aagggtccga agatcacgat catgaagttc aagaacaaga ccggctaccg caagcgccag
      601 ggtcaccgtc agcacctgac ccaggtcaag atcaccgcga tcgacgcctg agccactttc
      661 tcgaaaagga cttaagacat ggcacataaa aagggagcgt cctcgacccg caacggtcgt
      721 gactccaacg ctcagcgcct gggcgtcaag cgctacggcg gtcaggtcgt cgggaccggc
      781 gagatcatcg tccgccagcg cggcacccac ttccacccgg gcgacaacgt cggccgcggc
      841 ggcgatgaca cgctgttcgc cctcgccgac ggcaaggtcg agttcggcac caagcgcggc
      901 cgccgcgtcg tgaacgtcgt cgccggcgag tgacgcacta gctttccgta tgctgcgcgg
      961 cccagcccgc gcgcacctcg cgtgagtggg gcgggcccga gcggcccgcc ccctcgctgt
     1021 ttttcccctc gaccgacgct gagaggccga ttgcgatgac caccttcgtg gaccgggtga
     1081 ccctgcacgt caccgccggc gacggcggcc acggcgtcgc ctccatccgc cgcgagaagt
     1141 tcaagccgct cggcggcccc gacggcggca acggcggcaa cggcggcaac gtcgtcatca
     1201 ccgtcgaccc gcagtcgacc accctgctgg actatcaccg caacccccac cggcgcgccg
     1261 agaacggccg gcccggagcg ggcgacgagc gcgacggcgc gaaggggggc gacctcatac
     1321 tgcccgtccc ggagggcacg gtcgtctcca cccgcgacgg cgaggtgatc gccgacctcg
     1381 tcggcccggg tgcctccgtc gtcatcgccg aaggcggccg cggcggattg ggcaaccggg
     1441 ccctgtcctc gaccaagcgc aaggcgccgg gcttcgcgct gctgggcgag ccgggggaga
     1501 gcgtcgacat cgtgctcgag ctgaagtcgc tcgccgatgt cgccctgatc ggcttcccgt
     1561 ccgcgggcaa atcctctctc gtgtccgtca tcagtgcggc gcggcccaag atcgccgatt
     1621 acccattcac caccctcgtg ccgaacctcg gcgtcgtgac cgccgggtcg atgcgcttca
     1681 cggtcgccga cgtgcccggc ctgatccccg gcgcctccga gggccgcgga ctgggcctgg
     1741 agttcctgcg ccacgtcgag cgctgctcgg tgctcgtcca cgtcatcgac tgcgccaccc
     1801 tggagccggg ccgcgacccg ctgagcgatc tcgacgccat cgaggccgag ttggcggctt
     1861 atgtgcccgg cgacctgctc ggcggacggc cgctggccga gcgggtccgg atggtcgtcc
     1921 tgaacaaggc cgatgtcccg gaggcccgtg agctcgccga gatggtcgag cccgacctgc
     1981 gcgagcgcgg gtatgaggtg ttcatcgtct ccgccgtcgc gcacgtcggt ctgaaggagt
     2041 ggatcttcgc gatggcccgg gccgtcgagg ccgcgcgggc cgagcaggag tccgagatcg
     2101 tgccgcagcg cgtcaccatc cgtccgaaag ccgtggacga caagggattc caggtcgtgc
     2161 gcgagcaaga cggcggcgag accgtcttcc gagtcgtcgg cgacaagacc acccgctggg
     2221 tgcgtcagac cgacttctcc aatgacgagg cggtcggcta tctcgccgac cggctgcagc
     2281 gcgccggcgt cgaggacgcc ctcttggccg cgggcgccgt gcccggcgcg accgtgctca
     2341 tcggcccggc cgacaacgcc gtggtcttcg actgggatcc gaccatggtc ggcggcgccg
     2401 aactgctcgg tggccgcggc accgacggcc gcttcgacga tcgttcgcgc cggaccaaca
     2461 ccgaacgccg cgaggagttc cacgcccgca aggacgccca gaccgccgct cgcgaggagc
     2521 tggctgccga gcgcaaggcc gggcactggg ccacacccga caccgaccaa tgagcaccga
     2581 ccaatgagca ccgaccaatg agcaccgacc aatgagcacc gacgaactcg cgccggccga
     2641 actcgcggct gcccgagcgc gtattgccgg acgcggccgg atcgtcgtca aggtcggctc
     2701 gtcgtcgctg accaccgacg gcggccggcg tctggacccg gcccggatcg gggcgctcgt
     2761 cgcgcccctc gccgaccgcc ggctccgggg cgaacccatc gtcctcgtct cctccggtgc
     2821 catcgcggcc ggcatcggcc cgctcggatt ggcccgccgc ccaaaggatc tcgccaccca
     2881 gcaggcagct gcctcggtgg gccagggcgc actggtggcg gcgtacgccc aggccttcgc
     2941 ggcatacggc ctcacggtcg gccaggtgct gctcaccgcc gacgatgtga cccgacgctc
     3001 gcactacacc aacgcccgac gcaccctcga ccggctgctc gagctcggca tcgtgccgat
     3061 catcaacgag aacgacaccg tcgccaccca cgagatccgg ttcggcgaca acgaccggct
     3121 cgcagcgctg gtctcgcacc tggtcaaggc cgaggcgctc gtgctcctca ccgacgtcga
     3181 cgggctgtat gacggtccgc cgagtgagcc cgggacgcag cggatttcgc tgatccgctc
     3241 cctcgccgac ctggagaccg tgcgcatcgg cggcaccggg tcgatggtcg gcacgggcgg
     3301 catggcgacc aagatcgagg cggccgcgat cgccaactcg gcggggatca ccgccgtgct
     3361 gtgtgccgcg cccgccgtcg gtgaggcgct tgccggtgcg gacgtcggca cggtcttcct
     3421 gccgcactcg gtcggcaagg cgagccgcaa gctgtggatc gcccacgcca cgacacctcg
     3481 aggtgtcctg accgtggatg ccggggccgt cgcggcgctg gtcgagcggc gcaagtcgtt
     3541 gctgccggcc ggaatcatcg ctgtcacagg ggcattcgcc gacggcgacc ctgtcgatgt
     3601 ggcgggcccg gacggcgtcg tgatcgcccg aggcctcgtc agctatgacg cgggggagct
     3661 gcccaggatg atggggcgct ccactcggga actcgcgcgg gacctcggcc ccgaatacga
     3721 gcgcgaggtc attcaccgcg acaacctcgt cgtccgctga actcgccgga tcagccctcc
     3781 ggcagcacct ggacggcgcc gtcgcgggag ccgacgaagc cggtcccgga cgtcggcgta
     3841 aacccgcgct acctgaaccc gggctacttg ccgaggcggc gcttggcagc ctgcttgacg
     3901 cggcgagcct gggcaccggc ccagtcggcc gtgcgcaccg accggcggga cttcaggcgc
     3961 gcgacggtcg cctcgagctg ctcgatgcga cgctgctcga ccgtgagctt cgaccccgtg
     4021 gccttcgacc ccgtggcctt cgacgccgtg gccttcgacg ccgtggccgc cggcttcgct
     4081 gccgccgtct tgggcttggg ggtgtcgtcg aacatcggct cgtcgagcgg ggcgaagggc
     4141 ttgccgtcca tccgctcctc gatcaggacc cacggcatcc gccaccggtt caagatggtg
     4201 tagcggccgt cggggaagtt gtcgacgacg tcctgacgct cgaaacggcg cacatgggtc
     4261 ttcgagatcg gctccggctc ctcgaagggc acggtcatga gcaactgccc ccggcagacg
     4321 cggcgcagtt cggccaggcc ggcggtgaag atctcctcgg gaatgtgctc cagcacctcc
     4381 atgcacgtga cgacgtcgaa gtggtcgtcc ggatagggga gcttgtcgat gccgtcgttg
     4441 gtgcggtgga tcaggtcgga gtactcgatg tacttgccga accgggtcgg gtcggcgccg
     4501 tagacaccgg aatacttgcc ggacagcgcg accatgttga ggaactggcc cgccccgacg
     4561 ccgacgtcga gggcccgctc gccgtcgtcc atgcgggaca ggacgtatgc cgtgcgcggc
     4621 cagtcgtccc ggtggaacca cccgttcagt gtctccggca cctcgccata agtcccctcg
     4681 tagagcttct tgacctcgat ggccgtcccc tcgacggggt aggtcttcat ctcgaccact
     4741 gacgccttga actcggcggc ggcactcatc tgcttcctcg ctgactcggt gattgcgatg
     4801 ctccgcggtg agggtaccgc gccgaccggc aaaccctcat cgtctccagc cgcccacacc
     4861 gccgtgtcgc cgacagaccg gttgggcaac ggcacttgac ttaaagtcga cttcatcttt
     4921 caggatgggc ggcatgacct tgatcagcag ccggcccgtg acccgctcgg cgtccatcct
     4981 cagccccgaa caccggtggg tgaccatcgc cgcgatcgcc ctggtgaccc tcggcgccta
     5041 cgagaaccgc gccatcacca cgatcctgcc ggtgatcgct gacgacctcg acgggttgcg
     5101 ctggttcggc ctggcctcgg cgatccccgc cgcgacctac ttggtggcca ccacggtcgc
     5161 cggcacctgg accgaccaca ggggcccgcg tcggccgctg ttgttctcac tcgtgttgtt
     5221 cgccctcgcg cagatcgcgt ccggactcgc accctcgatg gaggtgctgc tcgccgcccg
     5281 ggcgctctcg ggcatcgcgg agggcggcct cgacatcgcg ctcgtcgtcc tggtgtccga
     5341 cctgatgccg gaagcgctgc gcgccaaggt cttcgcggcc ttcgctacgg cctggatcgt
     5401 gccgtcgctg gtcggtccgg cggtcgccgg cgggatcgcc caatggctcg gctggcgggc
     5461 ggccttcctg ctgcccctga tcatgttgat cgccgtcgtc cccgccctcc tcccggcgct
     5521 gcgacgggca accgcgcgcg ggg
//
LOCUS       DAOPJG010000212         5510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_15963_length_5510_cov_7.822365, whole genome shotgun sequence.
ACCESSION   DAOPJG010000212 DAOPJG010000000
VERSION     DAOPJG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15963_length_5510_cov_7.822365"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKU75_14820"
     CDS             <1..215
                     /locus_tag="PKU75_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF82197.1"
                     /translation="WVARSGELRHPMLIIHSRDDEFVPVGPSLELAAARPDLVTLVPW
                     EVARHCKEWNTDPLRWESEVAAFLRG"
     gene            complement(229..936)
                     /locus_tag="PKU75_14825"
     CDS             complement(229..936)
                     /locus_tag="PKU75_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPF82198.1"
                     /translation="MRLLSSDLHDLDLTETVRDDDLVRLYAFPERGGIRTNFVATLDG
                     AATGADGLTGSINNAADKVVFDLNRQLADVVVVGAATVDTEGYAPAAGKRPLIAVSNS
                     ALLPRGWSAGPQTPGMAVLVVPERADAAAVSRARDILGADNVWQMGADQVDLEELVAR
                     LRRDGLPRILSEGGPRLHTALLGLGLVDEVALTWVPTFAGGPAPRITADQQVTVDFRL
                     RHLLESDGTLLGLWSRR"
     gene            976..2073
                     /locus_tag="PKU75_14830"
     CDS             976..2073
                     /locus_tag="PKU75_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HPF82199.1"
                     /translation="MAKGKKNKDKKSRKEIEIIAKDLAVRKMLRGSKKSKNAKDGIAQ
                     AKVSELVAAVADGKASKSVVKGAPAPGVFEVALRAGDGFVLADVDPASTPGFIGDKAA
                     GEALMAPLAEQVADLQERLYAEAKEGGKRAVLLIIQGMDTSGKGGIMRHVVGAADPQG
                     IRITAFKAPTAEEKARPFLWRIRNALPTPGVIGVFDRSQYEDVLIGKVHELAPRATIG
                     RRYAQINSFEKSVAASGTKIVKVMLHISDEEQQARLAERLANPEKYWKFNPGDLGERA
                     LWGEYMDAYQVAIDKCSTEVAPWYVVPADKKWYARLAVMNLLLAALLDLDPQWPAADF
                     DVAEMQAKLEAMGDVTPDDPTDESSEDADES"
     gene            complement(2077..3720)
                     /locus_tag="PKU75_14835"
     CDS             complement(2077..3720)
                     /locus_tag="PKU75_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF82200.1"
                     /translation="MATHEVFNQPTPYGGDDLFRTDPTLVEAVERWGAPTADVARLGE
                     LAATAQAAEWATQADTMVPVLRSHDAQGRRVDEVVYHPAYHELMTVAVGRGLTAEPWL
                     ATPGSGAHLARAAGFYVWSQVEAGHLCPISMTYAAAPALAGQPDVSATWLRKLTARAY
                     DPRLAPVQDKHGAIVGMGMTEKQGGSDVRANTTAATADAAGPLGEHSHRLRGHKWFCS
                     APMSDAFLVLAQADEGLSCFLLPRVLPDGSRNAFAVQRLKDKLGNRANASSEVEFDGT
                     WAVPVGPLGRGVRSIITMVAATRLDCVLGSAATMRAALVRAAHFTAQRSAFGEVLIEA
                     PLMRNVLADLTLESEAATLLAMRLAHAVDEGEGDFARLAVALGKYWVCKRTAPMVAEA
                     LECLGGLGYVEENGMARLFREAPLNSIWEGSGNVNALDVLRAMTREPASVAALAKEAA
                     LARGVDRRLDAHIDAVESSAMTITGADALLQARALVERLAVTLQASLLVRYAEPVVAD
                     AFIASRVAGERGATFGTLPADLVRSAAPVLLARTPAGRR"
     gene            3779..4345
                     /locus_tag="PKU75_14840"
     CDS             3779..4345
                     /locus_tag="PKU75_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82201.1"
                     /translation="MNSLSAWHGGGMRSSAARGGLATVSILVAAPAGCGNAVTTDVVG
                     MTGVTADAQGRPVVIVAACGAAYDRIAVSDMLDRSTVPATQVNETLLTMTRETPLTGT
                     ETVSLTDPLAPWSASKRFEPTEPDGIDVAAFAEGEDRTTTSVMVTRAEYHQVSADVVL
                     VRQGERWTRAQFDARACDRDSWPEVTIS"
     gene            4415..>5510
                     /locus_tag="PKU75_14845"
     CDS             4415..>5510
                     /locus_tag="PKU75_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPF82202.1"
                     /translation="MVETKGAIKRAIAPIPGAMWLARIIAETVRICMRYRVTGLASEA
                     GFFALLSFPPLILGLFGGVGYIGRWLGEVTIESFTQQIGTYAGRFLTPDVIEQLLLPT
                     VSDVFANGRTDLISLGFVLSLWSGSRALNVFIDTISIMYGQSGVRGIVRTRVLSFTMY
                     VVTLIVGVVMIPLVLIGPTLLGRILPDRLDFLVILYWPVVTVLVVCSLTTLYHIATPK
                     RTPWVRDIPGAVVTLILWAIASYVVRGTISASLDGTSIYGPLSTPIVLLIWLYFIAIA
                     VLIGAAFNAATLRIQPAAREVVPFRERVKRPLTPFPEGKDPVERNIRFGYARLHGLRS
                     LSARTRETSRVPSADVAQLVERNLAKVEVAG"
BASE COUNT          831 a         1954 c         1893 g          832 t
ORIGIN      
        1 actgggtggc ccggtcgggc gaattgcgcc acccgatgct gatcatccac agccgcgacg
       61 acgagttcgt gcccgtcggg ccctccctcg aactggccgc agcccgcccc gatctcgtca
      121 ccctcgtccc gtgggaggtc gcccgccact gcaaggagtg gaacaccgat ccgctgcgct
      181 gggagagcga ggtcgcggcc ttcctgagag gttagggggc ggcgaacctc agcgacgcga
      241 ccacaggccg agcaaggtgc cgtccgactc gagcaaatgc ctcaacctga agtcgaccgt
      301 gacctgctga tcggcggtga tccgcggggc cggaccgccg gcgaaggttg ggacccaggt
      361 cagcgcgacc tcgtcgacga gccccagccc caagagggcg gtatgcagcc gcggcccgcc
      421 ttcgctgagg atccgcggca gaccgtctcg gcgcagccgc gccaccagct cctcgaggtc
      481 gacctggtcg gcacccatct gccagacgtt gtccgcgccg aggatgtcgc gcgcccggga
      541 cacggcggcg gcgtccgccc gctcggggac cacgagcacg gccatacccg gggtctgcgg
      601 accggcgctc cagccgcggg ggagcagcgc cgagttgctc accgcgatca gcggccgttt
      661 gccggccgcc ggggcatagc cctcggtgtc gaccgtcgcg gccccgacga ccacaacatc
      721 cgccagctgc cggttgaggt cgaagaccac cttgtcggcg gcattgttga tcgatccggt
      781 caggccgtcg gctcccgtgg ccgctccgtc cagcgtcgcg acgaagttgg tccggatccc
      841 gccgcgttcg gggaaggcgt atagccggac gaggtcgtca tccctcaccg tctcggtgag
      901 gtcgaggtcg tggaggtcgg aactcaagag ccgcacccgg ccagtgtggc gcatgcaagt
      961 gcaatgatgc cccttatggc aaagggcaag aagaacaagg acaagaagtc tcgcaaggag
     1021 atcgagatca tcgccaagga tctggcggtg cgaaagatgc tgcgcgggtc caagaagtcc
     1081 aagaacgcca aggacggcat cgcgcaggcc aaggtctccg aactcgtcgc cgccgtggcc
     1141 gacggcaagg cgtccaaatc cgtggtcaag ggcgctcccg cgcccggcgt cttcgaggtt
     1201 gccctgcggg ccggcgacgg cttcgtcctc gcggatgtcg accccgcgtc cacgcccggg
     1261 ttcatcggcg acaaggcggc cggtgaggct ctcatggcgc cgctggccga gcaggtggcc
     1321 gacctgcagg agcggctgta tgccgaggcc aaggagggtg gcaagcgcgc ggtgctgctg
     1381 atcatccagg gcatggacac ctccggcaag ggcggcatca tgcgccacgt cgtcggtgcc
     1441 gcggaccccc agggcatccg gatcaccgcg ttcaaggcgc ccaccgcgga ggagaaggcc
     1501 cgcccgttcc tgtggcgcat ccgcaacgcc ctcccgacgc ccggtgtcat cggcgtcttc
     1561 gaccggtcgc agtacgagga tgtcctgatc ggcaaggtcc acgagctcgc gccccgcgcc
     1621 accatcggcc gccgctacgc ccagatcaac tccttcgaga agtcggtcgc cgcctccggc
     1681 accaagatcg tcaaggtcat gctgcacatc tccgacgagg agcagcaggc ccgcctcgcc
     1741 gagcggctcg cgaacccgga gaagtattgg aagttcaacc ccggcgacct cggcgagcgc
     1801 gctctctggg gcgagtacat ggacgcctac caggtggcga tcgacaagtg ctccaccgag
     1861 gtcgcgccct ggtatgtcgt gcccgccgac aagaagtggt acgcccgcct cgccgtgatg
     1921 aacctgctgc tcgcggccct gctcgatctc gacccgcagt ggccggccgc ggacttcgac
     1981 gtcgcggaga tgcaggccaa gctcgaggcc atgggcgatg tcacccccga cgaccccacg
     2041 gacgagtcct ccgaggacgc cgacgagtcc tgactctcag cggcgccccg ccggcgtgcg
     2101 cgccagcagc accggcgcgg cgctgcgcac caggtcggcg gggagcgtgc cgaacgtcgc
     2161 gccacgttcg ccggccaccc gggaggcgat gaaggcgtcg gcgacgaccg gctcggcata
     2221 ccgcacgagc aggctcgcct gcagcgtcac ggccagccgc tccacgaggg cccgcgcctg
     2281 caggagcgca tcggccccgg tgatcgtcat cgcgctggac tcgacggcgt cgatgtgggc
     2341 atccaggcgc cgatcgaccc cgcgcgccag cgcggcctcc ttcgccagcg ccgccacgct
     2401 cgccggctca cgcgtcatcg cccgcagcac gtcgagggcg ttgacattgc ccgagccctc
     2461 ccagatggag ttcagcggcg cctcgcggaa cagccgcgcc ataccgttct cctccacata
     2521 accgagcccg ccgaggcact ccagggcctc ggccaccatc ggcgccgtcc gcttgcacac
     2581 ccagtatttg cccagcgcga cggcgagccg ggcgaagtca ccctcgcctt cgtcgacggc
     2641 gtgcgcgagc cgcatcgcga gcagcgtggc tgcctccgac tcgagcgtca agtcggcgag
     2701 gacgttgcgc atcagcggtg cctcgatgag cacctcgccg aacgccgagc gctgggcggt
     2761 gaagtgcgcc gcgcgcacca gcgccgcccg catcgtcgcc gccgacccga gcacacagtc
     2821 gagccgggtg gccgcgacca tggtgatgat cgagcgcacc ccgcgcccca gcggcccgac
     2881 cggcactgcc cacgtcccgt cgaactccac ctcggaggag gcgttggcgc gatttccgag
     2941 cttgtccttc agccgctgga cggcgaaggc gttgcggctg ccgtcgggca ggacgcgggg
     3001 gagcaggaag cagctgaggc cctcgtccgc ctgcgcgagt acgaggaagg cgtcgctcat
     3061 cggcgccgaa cagaaccact tgtgtccccg gaggcgatgg ctgtgctccc cgagcgggcc
     3121 ggccgcatcg gccgtcgcgg ccgtcgtgtt ggcgcgcacg tccgagccgc cctgtttctc
     3181 cgtcatgccc atgccgacga tggcgccgtg cttgtcctgg acgggcgcga gccgcgggtc
     3241 gtatgcccgc gcggtcagct tccgcagcca ggtcgcgctg acgtcgggtt ggccggcgag
     3301 tgcgggggcg gcggcatacg tcatggagat ggggcacagg tgccccgcct cgacctggct
     3361 ccacacgtag aacccggcgg cgcgggccag gtgggcgccg ctgccggggg tggcgagcca
     3421 gggctcggcg gtcaggccgc ggccgacagc cactgtcatc agctcgtggt atgccgggtg
     3481 gtagacgacc tcgtcgaccc gccggccctg cgcgtcgtgg ctgcgcagca ccgggaccat
     3541 ggtgtcggcc tgcgtcgccc actcggcggc ctgggcggtg gcggccaact cgccgaggcg
     3601 ggccacgtcg gcggtcgggg cgccccaccg ctcgaccgcc tcgacgaggg tggggtcggt
     3661 gcggaagagg tcgtcaccgc catacggcgt gggctggttg aacacctcgt gggtcgccat
     3721 gaccgcagga tagggcgggc gtccgcggct gccggcgcgg gtgagtacaa cgcctcatgt
     3781 gaactccctc tccgcctggc atggtggcgg catgcgctct tcagctgccc gtggcggcct
     3841 ggccaccgtc tcgatcctcg tggcggcgcc ggccggctgc ggcaacgcgg tgacgacgga
     3901 cgtcgtcggg atgaccggcg tgacggccga cgcgcagggc cggccggtgg tcatcgtggc
     3961 ggcctgcggg gcggcatacg accggatcgc ggtcagcgac atgctcgacc ggagcaccgt
     4021 cccggcgacc caggtcaacg agaccctgct gaccatgacg cgcgagacac ccctcaccgg
     4081 caccgagacg gtgtcgctca ccgacccgct cgcgccctgg agcgcgagca aacgcttcga
     4141 gccgaccgag cccgacggga tcgacgttgc tgcgttcgcg gagggggagg accggaccac
     4201 gacctcggtg atggtgacgc gcgcggaata ccatcaggtc agcgccgatg tggtgctggt
     4261 ccgccaaggg gagaggtgga ctcgcgcgca gttcgacgcg cgcgcctgcg accgcgacag
     4321 ctggccggaa gtgaccatct cgtgacccaa aaggtcttgc ggtgtgcgcc gtgtcgtcac
     4381 acggggatca cccgtaacac ctacgcttag cggcgtggtg gagacgaaag gagcaatcaa
     4441 gcgcgccatc gcaccgatcc ccggtgcgat gtggctggcc cggatcatcg ccgaaaccgt
     4501 gcggatctgt atgcgctacc gcgtcaccgg gctcgcctcc gaagccggct tcttcgccct
     4561 gctgtcgttc ccgccgctga tcctcggact gttcggtggc gtcggctaca tcggccggtg
     4621 gctcggcgag gtcacgatcg agtccttcac ccagcagatc ggcacctacg ccggccgctt
     4681 cctgacgccg gacgtcatcg agcagctgct gctgccgacc gtgagcgacg tgttcgccaa
     4741 cggccgcacc gacctgatct cgctcgggtt cgtgctgtcg ctgtggtcgg ggtcgcgggc
     4801 gctgaacgtc ttcatcgaca cgatcagcat catgtatggg cagtccggcg tgcgcgggat
     4861 cgtccggacc cgggtgctga gcttcaccat gtatgtcgtc acgctcatcg tcggtgtcgt
     4921 gatgatcccg ctggtgctga tcggcccgac gctgctgggc cggatcctgc ccgaccggct
     4981 cgacttcctg gtcatcctct attggccggt cgtcaccgtg ctcgtcgtct gctcgctgac
     5041 gacgctttac cacatcgcca cccccaagcg gaccccgtgg gtgcgcgaca tcccgggcgc
     5101 cgtcgtcacc ttgatcctgt gggccatcgc gtcgtatgtc gtgcggggca ccatctccgc
     5161 ctcgctcgac gggacctcca tctacgggcc cttgtcgacc ccgatcgtgc tgctcatctg
     5221 gctctacttc atcgccatcg ccgtgctcat cggtgccgcc ttcaacgctg ccactttgcg
     5281 catccaaccg gccgcgcgtg aggtcgttcc cttccgcgaa cgggtcaagc gaccgctgac
     5341 gccgttcccc gagggcaagg acccagtgga gcgcaacatc cgatttgggt atgcccgctt
     5401 gcatgggtta aggtctctga gcgcacgaac gagggaaacc tcgcgggtgc cgagtgcgga
     5461 tgtagctcag ttggtagagc gcaaccttgc caaggttgag gtcgccggtt
//
LOCUS       DAOPJG010000213         5491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_16020_length_5491_cov_7.718727, whole genome shotgun sequence.
ACCESSION   DAOPJG010000213 DAOPJG010000000
VERSION     DAOPJG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16020_length_5491_cov_7.718727"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PKU75_14850"
     CDS             complement(<1..805)
                     /locus_tag="PKU75_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HPF82203.1"
                     /translation="MTPEEFRAAGHQLIDWVADHRARIPDLPVAARTRPGEISGALPV
                     HAPQEPESLEAVLADLDAIVVRGVTQTQHPGFYGWFPSNASLSSVLGDIASGGLAALG
                     ITWQSAPALTEVEQVVTEWLRELTGLDPAWKGAIQDTASTGTLVAMLAARERASELSE
                     HAGGLQSVAQPLVVYSSAHAHSSVPKAVLLAGFGSDNLRFVDVDPTTYAMDPKALEAA
                     MAADVAAGRKPAAVIAALGTTGTTAFDPVAEIVPIAQRYGAWVHVDAAMA"
     gene            complement(978..1559)
                     /locus_tag="PKU75_14855"
     CDS             complement(978..1559)
                     /locus_tag="PKU75_14855"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPF82204.1"
                     /translation="MADTPPLADDFLALVGARIRARRRSKGVTVQQLADEAGISRRQL
                     TDVEQGHANPTLVTVTRIARALGTDFTELLEPADSGSVVDVIAPQGYTQVWSTEAGSS
                     ATLLVATPGARQADLWRWWLAPDEAYQGQADPAGSYELFSVLSGTLTIETDDGRYAVD
                     AGSSARLRSDRAYAYRNAGEVPVLFVRVVALAP"
     gene            complement(1561..1998)
                     /locus_tag="PKU75_14860"
     CDS             complement(1561..1998)
                     /locus_tag="PKU75_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF82205.1"
                     /translation="MSNDSVIMPPFHLAIPVGDIDEARHFYGEVMGFDRGRSDERWTD
                     WNVEGHQVVTHMVDEYAAPVAGWNHVDGHQVPVPHFGLVLSVERFQALAERMTQAGTD
                     FVIEPYVRFQGEVGEQWTMFFLDPSGNAMEFKAFADPSQLFAV"
     gene            2194..3102
                     /locus_tag="PKU75_14865"
     CDS             2194..3102
                     /locus_tag="PKU75_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPF82206.1"
                     /translation="MPVVTTLTELGGVATFRELARVHTRSQIRAAVSAAEVDLLARGR
                     YALPAVAEHRAAAHRHTAAQSHVSAAQAYGWKVKFPPGQPWLTFPLGRQVQATDIHVR
                     RAELTPQERAAGRTSPLRTVLDCARDLPYDEGLAVADSALRAGAVDPDRLRSTAATLR
                     GPGTAQARRIAGDADGRAANPFESVLRAIALEIPELAMRPQALLTDDGLFAQVDLADE
                     VLRLVLEADSYEHHGTQRGFTKDRRRYTELTAFGYHVLSFVWVDVMHHPAWVRWCIEC
                     WLATQLGRPKPPRPDRVPSARDLDGA"
     gene            3113..3385
                     /locus_tag="PKU75_14870"
     CDS             3113..3385
                     /locus_tag="PKU75_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82207.1"
                     /translation="MTQFPQIPVPEHGEQEAPRDSRRCSWCGASNFEPGFIEDSGQSS
                     RGFARWIVGDLEKGIFGGASRFGRERLDITGVRCSACGHLEFFANA"
     gene            complement(3470..>5491)
                     /gene="acnA"
                     /locus_tag="PKU75_14875"
     CDS             complement(3470..>5491)
                     /gene="acnA"
                     /locus_tag="PKU75_14875"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HPF82208.1"
                     /translation="VLGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEVKPGVTATD
                     VVLTITDLLRKHGVVGKFVEFYGAGVAALPLANRATIGNMSPEFGSTCAIFPIDEVTL
                     DYLRLTGRSEENVALVEAYAKEQGMWLDPAAEPRFSEKLELDLGTVVPSIAGPKRPQD
                     RIALSDAKMQFGIDVKNYGVDGELREEAVSGESGSYGLKDGAVVISSITSCTNTSNPS
                     VMMAAGMLAKNAVEKGLTVKPWVKTSMAPGSKVVTGYYEAAGLWPYLDKLGYNLVGYG
                     CATCIGNSGPLNEEISAAINKGDLAVTAVLSGNRNFEGRINPDVKMNYLASPPLVIAY
                     GLAGSMNFDFDLEPLGQDSAGNDVFLKDIWPSPEDVEATIAQAITRDLFEKDYADVFA
                     GDERWQSLPTPEGNTFEWDADSTYVRKPTYFDGMSMELTPVSDVEGARVLAKLGDSVT
                     TDHISPAGSIKADSPAGRYLADHGIGPKDFNSYGSRRGNHEVMIRGTFANIRLRNQLL
                     TDVEGGFTRDFTQDGAPQTTIYDASAAYQEAGIPLVILAGKEYGSGSSRDWAAKGTAL
                     LGVKAVIAESYERIHRSNLIGMGVLPLQYPQGQNAESLGLSGTETFTITGVTALNEGT
                     TPRTVKVVATREDGSNSEFDAVVRIDTPGEADYYRNGGILQYVLRSLVS"
BASE COUNT          823 a         1844 c         1964 g          860 t
ORIGIN      
        1 ccgccatggc ggcatcgacg tgcacccacg cgccataccg ctgcgcgatg ggcacgatct
       61 cggcgacggg atcgaaagcc gttgtgcccg tagtgccgag cgcggcgatg accgctgccg
      121 gtttgcggcc ggcggcgacg tcggcggcca tggccgcctc gagtgccttc gggtccatgg
      181 cgtaggtggt ggggtcgacg tcgacgaagc gcaggttgtc gctcccgaag cccgcgagca
      241 gcacggcctt gggcaccgag gagtgggcgt gcgccgagga gtagacgacg agaggttggg
      301 cgacactctg caggccgccc gcgtgttcgc tcagctcgga tgcgcgctcg cgggcagcaa
      361 gcatggcgac gagcgtgccg gtggaggcag tgtcctggat ggcgcccttc caggccgggt
      421 ccaggccggt cagctcacga agccactcgg tgacgacctg ctcgacctcg gtcagcgcgg
      481 gcgcggactg ccaggtgatg ccgagcgccg ccaagccgcc cgaggcgata tccccgagca
      541 ccgaggagag cgacgcgttg ctcgggaacc acccgtagaa gccgggatgc tgggtctggg
      601 tgacaccccg cacgacgatg gcgtcgaggt cggcgagaac ggcctcgagc gactccggct
      661 cctgcggcgc gtgcaccgga agggcgccgc tgatctcccc cggccgagtg cgcgccgcga
      721 cgggcaggtc cgggatgcgg gcgcggtgat cggcgaccca gtcgatgagt tggtgaccgg
      781 cggcgcggaa ctcttcgggc gtcataccgc cattgaagcc ctgcggcctg ggcgaggtcg
      841 cacgatctct ccttgtgagc accgacatct agcccctcgt ccgcaccgag cgcgaggctc
      901 tggcccgtga cctcgccgat ctcccgtacg atcccgaggc gttgcgcgcc gtcgcccggc
      961 tggatccccg ggttgactca cggggcgaga gcgacgaccc ggacgaacag cacgggcacc
     1021 tcgccggcgt tgcggtaggc ataggcgcga tcgctgcgca agcgggcgga ggagccggcg
     1081 tccacggcat accgtccatc gtctgtctcg atggtgagcg tgccgctcag caccgagaag
     1141 agttcgtagg agccggcggg gtcggcttgg ccttgatacg cctcgtcggg ggcgagccac
     1201 caccgccaca ggtcggcctg ccgggccccg ggggtggcca cgagcagcgt ggcgctgctg
     1261 ccggcctcgg tggaccacac ctgcgtatac ccctgtgggg caatgacatc cacgacgctg
     1321 ccggagtcag ccggctcgag cagttcggtg aagtcggtgc cgagcgcccg ggcgatgcgg
     1381 gtgacggtga ccagggtcgg gttggcgtgc ccctgttcga catcggtcag ctgccgccgg
     1441 ctgatgccgg cctcgtcggc cagctgctgg accgtgactc ccttgctccg ccgtcgcgcc
     1501 cggatgcggg caccgacgag ggcgaggaag tcgtcggcca gcggcggggt gtcggccacg
     1561 tcagacggcg aagagctggc tcgggtcggc gaaggccttg aactccatcg cattgccgct
     1621 cgggtcgagg aagaacatcg tccactgctc ccccacctcg ccctggaagc ggacatacgg
     1681 ctcgatgacg aagtcggtgc cggcctgcgt catccgctcg gcgagggcct ggaagcgctc
     1741 gacgctgagg accaggccga agtgcggcac cggcacctgg tgcccgtcga cgtgattcca
     1801 cccggctacc ggcgccgcat actcgtcgac catgtgcgtg accacctggt gcccctcgac
     1861 gttccagtcg gtccagcgct cgtcggagcg cccacgatcg aagcccatca cctcgccgta
     1921 gaagtggcgc gcctcatcga tgtcgccgac ggggatcgcc aggtggaagg gcggcatgat
     1981 gacgctgtca ttgctcatag tgagaactat agtgcgcaaa ccgggcgggt gccgaacatt
     2041 caaaatacga ggagccggcc gagagaaccg ctgctgagcg tgcagatgcc cacgagcgcg
     2101 cttcctcgta ttttgaatgt ccgggcggga gttatccaca gcgcgccgcg gcgtcccgag
     2161 acgaatcggc gatgtcgcgg aaggctgacc cacatgcccg tcgtcaccac gctcaccgaa
     2221 ttgggcggtg tggcaacctt tcgcgagttg gccagggtgc acacccgatc gcagatccgg
     2281 gcagcagtgt ccgcggcgga ggtcgacctg ctcgcgcgtg gccggtacgc gctgccggcc
     2341 gtcgccgagc accgggcggc ggcgcaccgg cataccgctg cccagtcgca cgtgagcgcg
     2401 gcgcaggcct acgggtggaa ggtcaagttc ccacccgggc agccctggct gaccttcccc
     2461 ctggggcgcc aagtccaggc cacggacatt cacgtgcgcc gggccgagct cacgccgcag
     2521 gagcgggccg ccggccgcac ctctcccctg cggacggtcc tcgactgcgc ccgcgacctg
     2581 ccctacgacg aaggcctcgc ggtcgccgac tcggccctgc gcgccggagc cgtcgacccg
     2641 gaccgattgc gctccacagc ggcgacactg cgcggacctg gcacggccca agcgcgccgg
     2701 atcgccggtg acgccgacgg gcgggccgcc aaccccttcg agtcggtgct gcgcgcgatc
     2761 gcgctggaga tacccgagtt ggccatgcgg ccgcaggcgc tcctcaccga cgatgggctg
     2821 ttcgcgcagg tggatctggc ggacgaggtc ctgcgcctgg tgctcgaggc ggacagctac
     2881 gaacaccacg ggacccagcg cggtttcacg aaggaccggc ggcgctatac cgaactgacg
     2941 gcgttcggtt atcacgtgct gtcattcgtc tgggtcgacg tgatgcatca cccggcgtgg
     3001 gtccgctggt gcatcgagtg ctggctcgcg acgcaactcg gccggcccaa gccgccccgc
     3061 cccgaccgcg tgccctcggc acgtgacctc gacggggcat agccttcacg gcatgacgca
     3121 gttcccccag atcccggtcc ccgagcacgg cgagcaggag gctccgcgcg acagtcggcg
     3181 ctgttcgtgg tgcggcgcaa gcaatttcga acccggtttc atcgaggaca gcggtcagtc
     3241 ctcgcgcggg ttcgcccgct ggatcgtcgg ggacctggag aagggcatct tcggcggcgc
     3301 ctcccggttc ggccgggagc gcctggacat cacgggcgtg cggtgcagcg cctgcggcca
     3361 cctcgagttc ttcgccaacg cctgacgccg ggagacggcc gacgggccgc acccgggttg
     3421 gggtgcggcc cgtcggtttc ggcggtatgc cgcggtgcgg cgctgcgggt cagctcacca
     3481 gggagcggag cacgtattgc aggatgccgc cgttgcggta gtagtcggcc tcgccgggag
     3541 tgtcgatccg cacgaccgcg tcgaactcgg agttcgagcc gtcctcgcgg gtggcgacga
     3601 ccttgaccgt cctgggagtg gtgccctcgt tgagcgcggt caccccggtg atcgtgaagg
     3661 tctcggtgcc ggacaggccg agggactccg cgttctggcc ctgcgggtac tgcagcggca
     3721 gcacccccat accgatcagg ttcgagcggt ggatgcgctc gtaggactcg gcgatgaccg
     3781 ccttgacccc gagcagcgcg gtgcccttgg ccgcccagtc ccgggaagac ccggagccgt
     3841 attccttgcc cgccaggatg acgagcggga tgccggcttc ctggtatgcc gcggacgcgt
     3901 cgtagatcgt cgtctgcggg gcgccgtcct gggtgaagtc gcgcgtgaac ccgccctcga
     3961 catccgtcag gagctggttg cgcaggcgga tgttggcgaa ggtgccgcga atcatgacct
     4021 cgtggttgcc gcggcgcgag ccgtaggagt tgaagtcctt cgggccgatg ccgtggtcgg
     4081 cgaggtagcg acccgccggc gaatcggcct tgatcgagcc ggccgggctg atgtggtcgg
     4141 tggtgaccga gtcgccgagc ttggccagga cgcgcgcgcc ctcgacgtcg ctgaccgggg
     4201 tcagctccat ggacatgccg tcgaagtagg tcggcttgcg gacataggtc gagtcggcgt
     4261 cccactcgaa ggtgttgccc tccggcgtcg gcagcgactg ccaacgctcg tctcccgcaa
     4321 agacatccgc gtagtccttc tcgaacaggt cacgggtgat cgcctgcgcg atcgtcgcct
     4381 cgacatcctc cggcgaaggc cagatgtcct tgaggaagac gtcattgccg gccgagtcct
     4441 ggcccagcgg ctcgaggtcg aagtcgaagt tcatcgagcc ggcgaggccg taggcgatga
     4501 ccagcggcgg ggaggcgagg tagttcatct tcacgtccgg gttgatccgg ccctcgaagt
     4561 tgcggttgcc cgagaggacc gcggtgacgg cgaggtcgcc cttgttgatc gccgcggaga
     4621 tctcctcatt cagcgggccc gagttgccga tgcaggtggc gcagccgtag ccgacgaggt
     4681 tgtagccgag cttgtccagg tagggccaca ggccggcggc ctcgtagtag ccggtgacga
     4741 ccttggaccc gggagccatg gacgtcttga cccacggctt gaccgtcagg cccttctcga
     4801 cggcgttctt ggccagcatg ccggccgcca tcatcacgct cgggttggag gtgttggtgc
     4861 aggaggtgat cgaggagatg acgacggcgc cgtccttcag cccatacgac ccgctctcac
     4921 ccgagacggc ctcctcgcgc agctcgccgt cgacaccata attcttgacg tcgatgccga
     4981 actgcatctt ggcgtcggag agggcgatcc ggtcctgcgg gcgcttgggc ccggcgatgg
     5041 acgggaccac cgtgccgagg tcgagttcga gcttctcgga gaagcgcggc tcggcggccg
     5101 ggtcgagcca catgccctgt tccttggcgt aggcctcgac gagcgcgaca ttttcctcag
     5161 agcggccggt caggcgcagg tagtcgagcg tgacctcgtc gatcgggaag attgcgcacg
     5221 tcgagccgaa ctccgggctc atgttgccga tggtggcgcg gttggccagc ggcagcgcgg
     5281 ccacgccggc gccgtagaac tcgacgaact tgccgaccac gccgtgcttg cgcaacaggt
     5341 cggtgatcgt caagacgacg tcggtcgcgg tgacgcccgg cttcacctcg ccggtcaact
     5401 tgaatccgac gacccgcggg atgagcatcg agaccggctg gcccagcatg gccgcctcgg
     5461 cctcgatgcc gccgacgccc cagccgagca c
//
LOCUS       DAOPJG010000214         5346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_16613_length_5346_cov_7.398034, whole genome shotgun sequence.
ACCESSION   DAOPJG010000214 DAOPJG010000000
VERSION     DAOPJG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16613_length_5346_cov_7.398034"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKU75_14880"
     CDS             complement(<1..554)
                     /locus_tag="PKU75_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPF82209.1"
                     /translation="MASTNSFGAQGQLQVGEQSYEIFRINTVEGHESLPYSLKVLLEN
                     LLRTEDGANVTADQIRALGGWDENADPDTEIQFTPGRVIMQDFTGVPCVVDLATMREA
                     VAELGGDPARINPLAPAELVIDHSVQIDVFGRADAFERNVEMEYERNGERYKFLRWGQ
                     TAFDDFKVVPPGTGIVHQVNIERLA"
     gene            complement(603..1913)
                     /locus_tag="PKU75_14885"
     CDS             complement(603..1913)
                     /locus_tag="PKU75_14885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPF82210.1"
                     /translation="MGGLAVGDRVEVEVGPVAHGGHCVARYEGQVLFVRHTLPGERVV
                     AEVTGIGSGNRFVYADARAVLTASPDRVVPPCPYAGRCGGCDFQHVALPAQRELKAAV
                     VREQLQRLARLDLPVEVEPVPGDRDGLRWRTRAEFAVSDDRRVGMRPHRSHEVLSIDD
                     CLIATDAVVGTGVLHRDPSTIAEDVGAFDVVAPSVGEPVVVPVPQDEAPDPVPDVAEH
                     VAARMLHRQFAVSARGFWQVHPGAPATFVDAVLEMLAPRPGERALDLYSGVGVFAAAL
                     AEAVGPVGQVIAIESDATATRHAEANLAAYRHVVAVRARVDDAFGLPRQVRGPRRGRT
                     RAGSRHPLLPHTADVVVLDPPRTGAGKDVCCAVAALSPRAIAYVACDPAALARDLATL
                     TGQGYAVTRLRSFDAFPMTHHVECLALLEPAGGRIDRTGPTKIS"
     gene            complement(1913..3871)
                     /locus_tag="PKU75_14890"
     CDS             complement(1913..3871)
                     /locus_tag="PKU75_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPF82211.1"
                     /translation="MRSDKLHETLLPKRIALPVFASDALSSVAYAPDEILLTLGMAGG
                     AMALTHSWQIALGVVFVMVVVVASYRQNVHAYPSGGGDYEVAGENLGPKAGLTVASAL
                     LVDYVLTVAVSVSSGVQNAASALTFVRGHEAIVAVVIVAILTAMNLRGVRESGSAFAI
                     PTYLFMFAVIGMAIVGFVRQAAGNLPYAESAALDVAPEEGFESLGTLALAFLLLRAFS
                     SGCAALTGVEAISNGVPAFQKPKSKNAATTLLMMGAIAVTMLFSMIMLARMTRVHFAE
                     DPGRQLLLNGVPVGDEYVQDTVMGQVSKAVFDGFPVGVVLVSTVTGLILFLAANTAFN
                     GFPVLASILARDGYLPRQLHTRGDRLAFSNGILILAGAACVLIIAYDAEVTRLIQLYI
                     VGVFVSFTLSQTGMVRHWNRLLRTERDPNERARMQRSRIINYIGAIICGLVLVVVLAT
                     KFIHGAGYAIAAMAVLFWIMTRIQNHYRHVSAELAVSEEETDHNRLPSRVHAIVLISR
                     LHKPALRALSYARATRPNRLEAITVNVDNEETAALQADWEKRGIPLPLKALDSPYREI
                     TRPIVDYVKSLRSDNPRDVVVVYVPEYVVGRWWEQILHNQSALRLKTRLRFMPGVMVS
                     SVPWQLRSSAGQEPVLSTSAPGDVRRGE"
     gene            3960..4625
                     /locus_tag="PKU75_14895"
     CDS             3960..4625
                     /locus_tag="PKU75_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPF82212.1"
                     /translation="MHFVIMGCGRVGASLARALERRGHDVAVVDQDEASFSRLGPTFE
                     GRTVTGIGFDRDTLLEAGVEQAYAFAAVSSGDNSNILAARVARETFGVEHVVARIYDP
                     GRAEIYQRLGIPTVATVKWTSDQMLRRLLPQGHLPELTDPSGKIVVAEVAVDPAWIGH
                     RLTSLETATGTRVAFYTRLGEGALPTPETILQEGDLVHLAINRDEIADVERILDSAPV
                     PHE"
     gene            4636..5325
                     /locus_tag="PKU75_14900"
     CDS             4636..5325
                     /locus_tag="PKU75_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPF82213.1"
                     /translation="MRVVIAGAGSVGRSIARELLYNNHSVLLIDKAADEHAIRRVPEA
                     NWLAADACEIDALNEAGLADCDVVVAASGDDKVNLVVSLLAKTEFGVPRTVARVNNPK
                     NEWMFDEAWGVDVAVSTPRLMTALVEEAVTIGDLVRIFEFKRGQATMVEMTLPADSPH
                     VGERVGDVEFPQDTVLVGIIRDQIPIAPSPDDSLEAHDELLFLTTPDSEDDLENMLSP
                     GQRIKRPRELA"
BASE COUNT          861 a         1828 c         1874 g          783 t
ORIGIN      
        1 gcgaggcgct cgatgttgac ctggtggacg atgccggtgc ccggcggcac gaccttgaag
       61 tcgtcgaagg cggtctggcc ccagcgcagg aacttgtagc gttcgccgtt gcgctcgtac
      121 tccatctcga cgttgcgctc gaaggcgtcg gcgcggccga agacgtcgat ctggacggag
      181 tggtcgatga ccaattcggc gggagcgagc gggttgatcc tggccgggtc cccgccgagt
      241 tcggcgacgg cctcgcgcat cgtggccagg tcgacgacgc agggcacgcc ggtgaagtcc
      301 tgcatgatca cgcggcccgg cgtgaactgg atctcggtgt ccgggtcggc gttctcgtcc
      361 cagccgccga gcgcgcggat ctggtcggcc gtgacattgg ccccgtcctc ggtgcgcagc
      421 aggttctcca gcagcacctt caggctgtag ggcagcgact cgtggccctc gacggtgttg
      481 atccggaaga tctcgtagct ctgctcaccg acctgcagct gtccttgtgc cccaaagctg
      541 ttcgtgctcg ccaccattgg ctccttccga cgccgtgcgt cttcgaccga gatatcttga
      601 cgtcaagata tcttagttgg tccggtcctg tcgatcctcc cacccgcggg ctccagcagg
      661 gcaaggcatt ccacgtggtg ggtcatcggg aaggcgtcga acgagcggag gcgggtgacg
      721 gcatacccct gcccggttaa ggtcgccagg tcgcgggcga gcgcggccgg atcgcaggcg
      781 acatacgcga tcgctctggg ggacaacgcg gccaccgcgc agcacacgtc cttgcccgcg
      841 cccgtgcgcg gcgggtcgag cacgaccacg tccgccgtat gcggcagcag cgggtggcgc
      901 gaaccggcgc gggtccggcc gcggcggggc ccgcggacct gccgcggcag gccgaaggcg
      961 tcgtcgacgc gcgcccgcac cgcgacgaca tggcggtatg ccgcgaggtt ggcttccgcg
     1021 tgccgggtcg ccgtcgcgtc ggactcgatc gcgatcacct ggccgaccgg cccgaccgcc
     1081 tcggcgagcg ccgcggcgaa gaccccgacg ccggaataga gatcgagcgc ccgctctccc
     1141 ggccgcggtg ccagcatctc cagcacggca tcgacgaagg tcgccggcgc gccggggtgg
     1201 acctgccaga acccgcgtgc cgagacggcg aactggcgat ggagcattcg ggcggcgaca
     1261 tgctcggcga cgtccgggac cgggtccggc gcctcgtcct gcggcaccgg cacgacgacg
     1321 ggctcgccca cactcggcgc gacgacgtcg aaggcgccga cgtcctcggc aatggtcgac
     1381 ggatcccggt gcagcacacc ggttcccacg acggcgtcgg tggcgatgag gcagtcgtcg
     1441 atcgacaaca cctcgtgcga gcggtgcggc cgcatcccga cgcggcggtc gtcggagacg
     1501 gcgaactcgg cgcgggtgcg ccaccgcaga ccgtccctgt cgccgggcac cggctcgacc
     1561 tcgacgggca ggtcgagccg cgccagccgc tgcagctgct cgcgcaccac cgcggccttc
     1621 aactcccgtt gggccggcag cgcgacatgc tggaagtcgc agccgccgca ccggccggca
     1681 tacgggcagg gcgggaccac gcgatcgggt gacgcggtca ggaccgcacg cgcgtcggca
     1741 tacacgaaac ggttgccgga cccgatgccg gtgacctcgg cgaccacccg ctcccccggc
     1801 agtgtgtgcc gcacgaagag cacctgcccc tcataccggg ccacgcagtg cccgccatgg
     1861 gcgaccgggc cgacctcgac ctcgacgcgg tccccgaccg caagcccgcc gatcactcgc
     1921 cgcgccggac gtcgccgggc gcggacgtcg agagcaccgg ctcttgaccg gcggaggaac
     1981 gcagctgcca gggcacgctg gagaccatga cgccgggcat gaaccgcagc cgggtcttca
     2041 ggcgcagggc ggactgattg tgcaggatct gctcccacca gcggccgacg acatattcgg
     2101 ggacgtagac caccacgaca tcacgcgggt tgtccgaccg cagcgacttg acgtagtcga
     2161 cgatcggccg ggtgatctcc cggtatggac tgtcgagggc cttcaacggc agcggtatgc
     2221 cgcgtttctc ccaatccgct tgcagcgccg cggtttcctc gttgtcgacg ttgacggtga
     2281 tcgcctcgag ccggttgggg cgggtggcgc gggcatacga cagcgcgcgc agcgcgggtt
     2341 tgtgcagccg ggagatcagc acgatcgcgt ggacccgcga cggcagacgg ttgtggtcgg
     2401 tctcctcctc gctcaccgcg agttcggcgc tgacgtgccg gtagtggttc tggatgcggg
     2461 tcatgatcca gaagagcacc gccatggccg cgatcgcgta gcccgccccg tggatgaact
     2521 tggtggccag gacgacgacg agcaccaggc cacagatgat ggcgccgatg tagttgatga
     2581 tccggctgcg ctgcatccgg gcccgctcgt tcgggtcccg ctcggtgcgc agcaaccgat
     2641 tccagtgccg caccatgccc gtctggctga gggtgaagga gacgaagacg ccgacgatgt
     2701 aaagctggat gagccgggtc acctcggcgt cgtaggcgat gatcagcacg cacgccgcgc
     2761 cggcgaggat gagtatgccg ttggagaagg ccagccggtc gccccgggta tgcagttgcc
     2821 tcgggaggta gccgtcgcgc gccaggatcg aggcgaggac cgggaagccg ttgaaggcgg
     2881 tgttggcggc caggaagagg atcagtccgg tcaccgtcga gacgaggacg accccgacgg
     2941 gaaagccgtc gaagaccgcc ttggagacct ggcccatgac ggtgtcctgg acgtattcgt
     3001 cgccgacggg cacgccgttg aggagcagct gtcggcccgg gtcctcggcg aagtgcaccc
     3061 gggtcatccg cgccagcatg atcatcgaga agagcatcgt gacggcgatg gcgcccatca
     3121 tgagcaaggt ggtggcggcg ttcttgctct tgggtttctg gaaggccggc acgccattgc
     3181 tgatggcctc gacgccggtg agggcggcgc agccggagga gaaggcgcgc agcagcagga
     3241 aggcgagcgc cagggtgccg agggattcga agccctcctc gggcgcgaca tccaaggccg
     3301 cgctctcggc atacggcagg ttgccggcgg cctgccggac gaagccgacg atcgccatcc
     3361 cgatgacggc gaacatgaac agataggtgg ggatggcgaa ggcggacccg gactcgcgaa
     3421 cgccgcgcag gttcatggcg gtcaggatcg cgacgatgac gacggcgacg atcgcctcgt
     3481 gcccgcgcac gaaggtcaag gcgctggcgg cgttctgcac acccgaactg accgacaccg
     3541 ccaccgtcag cacgtagtcg acgagcaggg cgctcgcgac ggtcaggccg gccttggggc
     3601 cgaggttctc gcccgccacc tcgtagtcac cgccgccgga ggggtaggcg tgcacattct
     3661 gccggtagga cgcgacgacg acgaccatga cgaacacgac gccgagggcg atctgccacg
     3721 agtgggtcag ggccatcgcg ccgccggcca taccgagggt cagcaggatc tcgtcggggg
     3781 cataggcgac cgaagacagg gcgtcgctgg cgaagacggg tagcgcgatg cgcttgggga
     3841 gcaaggtctc gtgcagcttg tcgctgcgca tcgcctcgcc caggagaatg cgtttgagca
     3901 cgcgcccgat ggccacgacc gtcaactcta atcccccgcg cccggtgtag cgtcggagcg
     3961 tgcatttcgt catcatggga tgcggtcgtg tgggcgcctc gctcgcccgg gcgctggagc
     4021 ggcgcggcca cgatgtcgcg gtcgtcgatc aggatgaggc gtccttcagc cggctggggc
     4081 cgaccttcga aggccgcacc gtcaccggca tcggtttcga ccgggacacc ctcctcgagg
     4141 cgggtgtcga gcaggcctac gccttcgccg cggtctccag cggtgacaac tccaacatcc
     4201 tcgccgcccg cgtcgcgcgc gagacgttcg gcgtcgagca tgtggtggcc cgcatctacg
     4261 acccggggcg cgcggagatc taccagcgcc tcggcatacc caccgtggcg accgtgaagt
     4321 ggacctccga tcagatgctg cgccggctgc tgccgcaggg gcacctgccc gagctgaccg
     4381 atccgtccgg caagatcgtg gtcgccgagg tcgccgtcga cccggcatgg atcgggcacc
     4441 ggttgacctc gctcgagacc gcgacgggga cccgggtggc gttctatacc cggctcggcg
     4501 agggtgccct gcccactccg gaaaccattc tccaagaagg ggatctggtg cacctggcca
     4561 tcaaccgtga cgagatcgcc gatgtcgagc ggatcctcga cagcgctccc gtcccccacg
     4621 agtgagagac cactgatgcg cgtcgtcatc gccggagccg ggagcgtggg ccgctccatc
     4681 gcccgcgaac tgctctacaa caaccactcc gtgctgctga tcgacaaggc ggccgacgag
     4741 catgcgatcc ggcgggtgcc ggaggccaac tggctcgcgg ccgacgcctg cgagatcgac
     4801 gcgctcaacg aggccgggct cgccgactgc gacgtcgtgg tcgccgcatc cggtgacgac
     4861 aaggtcaacc tcgtcgtctc gctcctcgcc aagaccgagt tcggggtgcc ccgcaccgtg
     4921 gcgcgggtga acaaccccaa gaacgagtgg atgttcgacg aagcgtgggg cgtcgatgtc
     4981 gccgtctcca ccccccgcct gatgacggcc ctggtcgagg aggccgtgac gatcggtgac
     5041 ctggtgcgca tcttcgagtt caagcgcggc caggcgacga tggtcgagat gaccctgccg
     5101 gccgactcgc cgcacgtcgg cgaacgcgtc ggcgatgtcg agttcccgca ggacaccgtc
     5161 ctcgtgggca tcatccgcga ccagatcccc atcgctccca gccccgacga cagcctcgag
     5221 gcccacgacg agttgctctt cctcaccacc cccgattccg aggacgacct ggagaacatg
     5281 ctctcccccg gccagcgcat caagcgcccg cgggaactcg cctagctcgc cgaaccccct
     5341 gccccc
//
LOCUS       DAOPJG010000215         5210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_17247_length_5210_cov_7.530747, whole genome shotgun sequence.
ACCESSION   DAOPJG010000215 DAOPJG010000000
VERSION     DAOPJG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17247_length_5210_cov_7.530747"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKU75_14905"
     CDS             <1..729
                     /locus_tag="PKU75_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HPF82214.1"
                     /translation="STWYVNAKHKDAAQIGVTSYRRDLPATATLEEVLATVDELNQEP
                     GCTGFLVQQPTGLDENAILSRVDPAKDVDGLHPTNLGWLVLGKEAPLPCTPKGCIELL
                     RRYDVPLAGARVAIVGRGITVGRPLGLMLTRRSENATVVTCHTGTKDLAGELRRADVI
                     IAAAGVPGIVTADMVQPGAAVLDVGVSRITAEDGHSVVAGDVDKSVWDVAGWVSPNPG
                     GVGPMTRAMLLSNVVEAAERAACA"
     gene            762..1133
                     /locus_tag="PKU75_14910"
     CDS             762..1133
                     /locus_tag="PKU75_14910"
                     /inference="COORDINATES: protein motif:HMM:NF022668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HPF82215.1"
                     /translation="MSLDPEEQPAAGASAAESPWGEGKVRPVQPEPRPPAVTRLGPWW
                     LVALGVAGGIVWAATDHMLRATVTLGSACLLGAGLRLVLPARRAGGLITRSRPFDVLV
                     LLILGAALLAAGFALDLRARV"
     gene            1213..1872
                     /locus_tag="PKU75_14915"
     CDS             1213..1872
                     /locus_tag="PKU75_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF82216.1"
                     /translation="MGGWLRVGLIDDHPAISTGVPAGLGQVIDPAPVFTDAKTVDELV
                     TSEVGADGFDVVILDVRLADESTPEDNVRRLSERGWPVLLYTREPRPAVLARCFAAGA
                     RGAVGKHEDWPVLAEALHAVAAGELYYNAEWACAVEAMSDSHRFPELAPRELEAVQLY
                     AAGIPAKSVARRMGVSETTVKEYLRRAGKKYEDAGRPAATKTGLAQRAMEDGFYFPVK
                     E"
     gene            1869..4145
                     /locus_tag="PKU75_14920"
     CDS             1869..4145
                     /locus_tag="PKU75_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82217.1"
                     /translation="MSVPAEQSPHQIIWVAVSWAGIAIVGLNLPVTVIDVAGSRTAYV
                     PWFAEIVCVLYAALWVSALVLILRRRRARKAMQAIVILSLAVLALHPLTLRTGGSQYP
                     PLLHLVGAGLCLSAVVHLRLATTLIVPAASAAVALIRIPELGVGRAWGEAALLAVSAW
                     LATFAIRIVVRANDSVDDAVAAAARASERELRARRQTYERLRWNGLIHDKVLAALRAA
                     SRGAGDTIPRDAVALAATGLTALTGATQPRPGALAAIWREHAERLGLEVAVDVTGDVP
                     DPEVRLALRDATREALTNVQRHSGQRTAVLHGILTPTRATVTVTDPGRGFRVSETGHR
                     AGIATSIVARMRAVGGHAHVRSTPQHGTEVRLDWSAAGPTAASQDWQLGLFAPIMVVG
                     AIAFLLNVFLAIDQWRLSPAISLTVAAIVVLAGLTVATTLLTPSDWNWVPLAVLAVVT
                     IAALAGTIPQGAPESWRYWFLGALTPAVGAMAFRFRPWAGLALAGAATLVCMVADALA
                     GRSVWQCLVGPVPVLITTAAAGQLVRTAFDSAYVRTAEAMAEDIQARLATAVSEERAL
                     EDDRRTEALQASIGPALQLIAGGGRLTPALRDQLADLESAARDQLAAPDLVDPALAAT
                     LARARARGIRIDIVDTSPTDAVTGGEESLDLCRRLVADLAEGVPPHTRVRINWHAGSG
                     GAGATFSAAARDLLDKTPNLAQALADLAGRQARVSTDSDSILIRCETPAKQARPRERS
                     LATVPTDAKYLGRHARLR"
     gene            complement(4138..5124)
                     /locus_tag="PKU75_14925"
     CDS             complement(4138..5124)
                     /locus_tag="PKU75_14925"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPF82218.1"
                     /translation="MSTPVKVAVTGAAGQIGYSLLFRLASGALLGPDTPIELRLLEIT
                     PALKALEGVVMELDDCAFPTLAGVQIGDDAKKVFDGVNLALLVGARPRGPGMERGDLL
                     EANGGIFAPQGKALNEVAADDIRVTVTGNPANTNALIAMSNAPDIPQSRFSALTRLDH
                     NRAISQLAAKLGVPVTEITKMTIWGNHSATQYPDLFHAEVSGRNAAEAVGDQDWLANT
                     FIPTVAKRGAAIIEARGSSSAASAASATIDHARTWLQGTPEGDWVSMSVVSDGSYGVA
                     EGLISSFPVTVANGEWSIVQGLDIDDFSRGKIDASVAELAEEREAVKELGLI"
BASE COUNT          726 a         1862 c         1865 g          757 t
ORIGIN      
        1 agcacgtggt atgtcaacgc caagcacaag gacgccgccc agatcggcgt gacgagctac
       61 cgccgcgacc tgcccgccac ggccaccctc gaggaagtcc tcgccaccgt cgatgaactc
      121 aaccaagagc ccggctgcac cggcttcctg gtccagcagc cgaccgggct ggacgagaac
      181 gccatcctca gccgcgtcga cccggccaag gacgtcgacg gactgcaccc gaccaacctc
      241 ggctggctgg tcctgggcaa ggaggccccg ctgccgtgca cccccaaggg ctgcatcgag
      301 ttgctccgac ggtatgacgt gccgctggcg ggtgcccgcg tcgccatcgt ggggcgcggg
      361 atcacggttg gccggccgct gggcctgatg ctcacccgcc gctccgagaa cgccacggtc
      421 gtcacatgtc acaccggcac caaggacctc gccggcgagc tgcgccgggc cgacgtgatc
      481 atcgccgccg ccggggtgcc gggcatcgtc accgccgaca tggtccagcc gggagccgcg
      541 gtcctcgacg tcggcgtcag ccggatcacc gccgaggacg gccacagcgt cgtcgccgga
      601 gacgtcgaca agagcgtctg ggacgtcgcg ggctgggtca gccccaaccc cggcggcgtg
      661 ggcccgatga cgcgggcgat gctgctgagc aatgtcgtcg aggccgccga acgcgcggcc
      721 tgcgcctgag taccacgacc cgcgcacacc gcatacccac gatgtcactt gacccggagg
      781 aacagccggc cgcaggagcc agcgcggccg agtcaccgtg gggcgagggc aaggtccgtc
      841 cggtgcaacc ggagccccgg ccgccggcgg tcacccggct cggcccgtgg tggctggtcg
      901 cccttggcgt cgcgggggga atcgtctggg ccgcaacgga tcacatgctc cgggccacgg
      961 tgaccctcgg gtccgcctgc ctcctggggg ccggcttgcg gctggtgttg cccgcgcgcc
     1021 gggcgggtgg actgatcacc cgcagccgcc ccttcgacgt gctcgtgctg ctcatcctgg
     1081 gcgcggcgct gctggccgcc ggattcgctc tggatctgcg ggcccgcgtc tgatggttgt
     1141 cctccagatg gaggacagga gagcatcgtc gttccggacc cgctcgcccc ggcaacagag
     1201 catgatcgac tcatgggagg gtggcttcgg gtgggcttga tcgacgatca tccggcgata
     1261 tcgacggggg ttcctgcggg tctgggccag gtgatcgatc ccgcgccggt cttcaccgac
     1321 gccaagaccg tggacgaact ggtgaccagc gaagtgggcg ccgacggttt cgacgtcgtc
     1381 atcctcgacg tccggctggc cgacgagagc acgcccgagg acaacgtgcg ccggctcagc
     1441 gaacgcggct ggccggtgct gctctacacc cgcgagccgc gcccggccgt tctcgctcgc
     1501 tgcttcgcgg ccggcgcgcg cggcgcggtc ggcaagcacg aggactggcc ggtcctcgcc
     1561 gaggccctgc acgccgtcgc cgcgggggag ttgtactaca acgcggagtg ggcgtgcgcc
     1621 gtcgaggcga tgagcgactc gcaccgcttc cccgagctgg cgccacggga gctggaggcc
     1681 gtgcagctgt atgccgcggg catcccggcc aagtccgtgg cccgccggat gggcgtcagc
     1741 gagacgacgg tcaaggagta tctgcgccgg gccggcaaga agtatgagga cgccggccgg
     1801 ccggccgcga ccaaaaccgg tctcgcgcaa cgagcaatgg aggacggttt ctacttcccg
     1861 gtcaaggaat gagcgtcccc gccgaacagt cgccgcacca gatcatctgg gtcgcggtgt
     1921 cctgggcggg catcgcgatc gtcgggctga acctgcccgt cacggtcatc gacgtggccg
     1981 gctcgcggac cgcgtatgtg ccgtggttcg ccgagatcgt ctgcgtgctg tatgccgcgc
     2041 tgtgggtcag tgcgctggtc ctgatcctgc gccgccgcag ggcccgaaaa gcgatgcagg
     2101 ccatcgttat tctctcactc gccgtgctgg cgctgcaccc gctcacgctg cgcaccggcg
     2161 gctcgcagta ccccccattg ctgcacctcg tcggtgccgg actctgcctg tcggccgtcg
     2221 tccacctgcg actggccacg acgctcatcg tgccggctgc cagcgccgcg gtcgcgttga
     2281 tccgcatccc cgaactgggc gtaggacgcg cttggggcga ggccgcgctc ctcgccgtca
     2341 gcgcttggct cgccaccttc gccatccgga tcgtggtgcg cgccaacgat tccgtcgacg
     2401 acgccgtggc ggccgcggcc cgcgccagtg agcgggagtt gcgggcccgg cggcagacct
     2461 atgagcggtt gcgctggaac gggttgatcc acgacaaggt gctggcggcg ctgcgcgcgg
     2521 ccagccgcgg cgccggcgac accatcccgc gggacgcggt ggcgctggcc gcgacgggac
     2581 tgacggcgtt gacgggcgcg acgcagccgc gcccgggggc cctggccgcg atctggcgcg
     2641 agcacgccga acgtctcggg ctcgaggtgg cggtcgatgt caccggcgac gtccccgacc
     2701 cggaggtgcg gctggctctg cgggacgcaa cccgcgaagc gctgaccaac gtccagcggc
     2761 actccggaca gcgcacggcc gtgctgcacg gcatactcac ccccacccgt gccacggtga
     2821 ccgtgaccga tcccgggcga gggttccgcg tctccgagac gggccatcgg gccggcatcg
     2881 ccaccagcat cgtcgcccgg atgcgggccg tcggtggcca cgcccacgtg cgcagcacgc
     2941 cccagcacgg caccgaggtc cggctcgact ggtcggccgc gggccccacc gccgcctccc
     3001 aggactggca gctcggcctg ttcgccccga tcatggtggt cggcgcaatc gccttcctgc
     3061 tcaacgtctt tctcgccatc gaccagtggc gactgagccc cgctatcagc ctgacggtgg
     3121 ccgccatcgt cgtcctcgcc gggctgacgg tcgccaccac cctgctcacc ccgagcgact
     3181 ggaactgggt gccccttgcc gtcctcgcgg tggtcacgat cgccgcgctc gccggcacga
     3241 tcccgcaggg ggcgccggag tcgtggcgct actggttcct gggcgcgctc accccggctg
     3301 tgggcgcgat ggccttccgg ttccggccct gggccggact ggcgttggcc ggcgcggcga
     3361 cgctggtctg catggtggcg gacgctctgg ccggccgcag cgtctggcag tgcctggtcg
     3421 gtcccgtgcc ggtgctgatc accaccgctg ccgccggcca gttggtgcgc acggcgttcg
     3481 attcggcgta tgtccgcacg gcggaggcga tggccgagga catccaggcg cgtctggcga
     3541 cggcggtgag cgaggagcgt gcgctcgagg acgaccgtcg caccgaggcg ctgcaggcga
     3601 gcatcggtcc ggctctgcag ctcatcgccg gcggcggccg gctgaccccg gcgctgcggg
     3661 atcaactggc cgacctggag tcggccgccc gcgaccaact ggccgcaccc gacctggtcg
     3721 acccggcgct cgccgccacc ctggcgcggg cgcgagcgcg cggcatacgc atcgacatcg
     3781 tcgacaccag ccctaccgac gcggtgacgg gcggcgagga gagtctcgat ctgtgccggc
     3841 gcctggtcgc cgacctcgcc gagggagtgc cgccccacac gcgggtgcgc atcaactggc
     3901 acgctggcag cggcggtgcc ggggcgacct tcagcgctgc cgcgcgcgat ctcctcgaca
     3961 agacgccgaa cctcgctcag gcgttggccg acctcgccgg ccggcaggcc cgggtcagca
     4021 ccgacagcga cagcatcctg atccgctgcg agacgcccgc caaacaggcg cgtccgcggg
     4081 aacggtcgct agcaaccgtt cccacggacg cgaagtacct gggcagacac gccaggctca
     4141 gatgaggccg agctccttga cggcctctcg ctcctcggcc agctcggcca cggaggcgtc
     4201 gatcttgccg cgggagaagt cgtcgatgtc taggccctgc acgatcgacc actcgccgtt
     4261 ggcaaccgtc acggggaagg aggagatgag cccctcggcc acaccatagg agccgtcgga
     4321 caccacggac atcgacaccc agtcgccctc aggcgtgccc tgcagccacg tgcgggcgtg
     4381 gtcgatcgtg gccgacgccg cggacgccgc cgaggaggac ccgcgcgcct cgatgatggc
     4441 cgcgccgcgc ttggcgacgg tcgggatgaa ggtgttggcc agccagtcct ggtcgccgac
     4501 ggcctcggcc gcgttgcgcc cggagacctc ggcgtggaac aggtcggggt actgcgtcgc
     4561 cgagtggttg ccccagatcg tcatctttgt gatctcggtg accggcacac cgagcttggc
     4621 ggcgagctgg gagatggcgc ggttgtggtc gagccgcgtc agggccgaga accgcgactg
     4681 ggggatgtcc ggcgcgttcg acatcgcgat gagcgcgttg gtgttggccg ggtttccggt
     4741 gacggtgacg cgaatgtcgt ctgccgcaac ctcattgagc gccttgccct gcggggcgaa
     4801 gatgccgccg ttggcctcga gcaggtcgcc gcgctccatg cccgggccgc gcgggcgggc
     4861 gccgacgagg agggcgaggt tgaccccgtc gaagaccttc ttggcgtcgt cgccgatctg
     4921 cacgccggcg agggtcggga aggcgcagtc gtcgagttcc atgacgacgc cctcgagcgc
     4981 cttcagcgcg ggggtgatct ccaacaggcg cagttcgatc ggggtgtccg ggccgagcag
     5041 cgcgccgctg gcgagacgga acaggaggct gtagccgatc tggccggcgg cgccggtgac
     5101 ggcgaccttg acgggagtac tcacagtgct tctcaacctt ttcgagtcga cgacgggagt
     5161 atgcagcgca gccctgccgc accgcatacc ggatgctccg actctagtcg
//
LOCUS       DAOPJG010000216         5166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_17449_length_5166_cov_7.880258, whole genome shotgun sequence.
ACCESSION   DAOPJG010000216 DAOPJG010000000
VERSION     DAOPJG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17449_length_5166_cov_7.880258"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..237
                     /locus_tag="PKU75_14930"
     CDS             19..237
                     /locus_tag="PKU75_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82219.1"
                     /translation="MLGPNGAGKTTTADWLARTSGRVRPTCSLRGAWSSPSNGSTRTP
                     RRGSSSSRSSPRRGPCSRDGAPSRSPSW"
     gene            363..1127
                     /locus_tag="PKU75_14935"
     CDS             363..1127
                     /locus_tag="PKU75_14935"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPF82220.1"
                     /translation="MANEASARAKTIDELRAAQAKLAAAERESGVAQERERLSREIHD
                     TLAQGFTSVLTLTRAARAALGRGDVETAIARLDLIEQTAGDNLHEARLMVAELTPGHL
                     QSRSLAEALERLAATVSAESGLRVEMQTRGMPIALGGATDVVLLRTAQEAITNARRHS
                     GARHVTMQLVYGEDVRLIVTDDGRGFDTSTPRRGFGLDGIQSRAQAIGGTVEVRSASD
                     HGTTIDVALPAEPDPVAAEPDPGRTGPPPSLSRSES"
     gene            1124..1771
                     /locus_tag="PKU75_14940"
     CDS             1124..1771
                     /locus_tag="PKU75_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF82221.1"
                     /translation="MTTPAVTRIRLVVADDHPVVRAGLVALLSEEPDFEVVGQAADGA
                     AAIVAVTEHHPDLVLMDLRMPVLDGAAAAAAITALPDGPPVLILTTYDTDADIVRAVE
                     AGARGYLLKDAPPEVLSAAVRSAARGETVLAPAIAGRLAERLRKPAAADLTTRELDVL
                     RLVAEGLSNAEIGSRLFIGEATVKTHLIRAFQKLEVADRTAAVTEAYRRGLLTIE"
     gene            complement(1758..2561)
                     /locus_tag="PKU75_14945"
     CDS             complement(1758..2561)
                     /locus_tag="PKU75_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPF82222.1"
                     /translation="MATIVFFHAHPDDEASQTSGSMARAAAQGDRVVVVYATNGEHGE
                     VPDDLLPGETLVERRRKEATKSAAALGTARIEWLGYSDSGMTGWDQNAHVGAFHTATL
                     TDAGMRLAQILDREDADVIVGYDWHGGYGHPDHVKVHHVLHAAAKLAARPPRVLESTM
                     NRDVTRRLYQEAVAAGATEEQAWDPDQPMDDGNPLGSNEYEITWAVDVHKFLPQKRAA
                     LAAHASQKTDIESLLSMPEEAFTAAFGTEYYIEPALGPGMRDGWPFTRS"
     gene            complement(2566..2988)
                     /locus_tag="PKU75_14950"
     CDS             complement(2566..2988)
                     /locus_tag="PKU75_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPF82223.1"
                     /translation="MPVADEPLDVLLGRALRQLRHQWGAGVGEVDLAPHQARALRIVH
                     EDGPLRLSELARRLRIVPRSATEVADALQERGLVLRSPDPSDRRATLLEMTGHGRSVA
                     TEVGRMRREQAEAFFGRLSANDRASLHRILTELLDQGE"
     gene            3090..4934
                     /locus_tag="PKU75_14955"
     CDS             3090..4934
                     /locus_tag="PKU75_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF82224.1"
                     /translation="MNEDVNLGGRGGGRGPQKLDPADRAQLALSPVDPRRILALFAPH
                     RLALAIVVAIIVASSAIALAQPFLVKAVIDDALPHQNVRLLLLAVGGMLAVTVTTALL
                     GVAQTWISTRVGQEVMHRLRTDVFAHLQKQSLGFFTRTRGGEVQSRIIQDIGGMQSVV
                     TSTATSIASNLTTAVGTAIAMAALSWRLSLLSLIVMPPAIWLTRRVALLRKEITAQLQ
                     RRLADMHVQVDEGLSVSGVLLTKTLGAGAQATERFTSTSTELVDLELRAQLAGRWRMA
                     TMAIIFAIIPALLYLAAGFPATSGGMTIGTLVAFTALQAGLFRPVMGLLNVGVQWVSS
                     MALFSRIFGYLDLVTDVPAPIAPVAVDHHRVRGDVRLDGVSFAYEAGGTPVLHEITLS
                     VPAGSSLALVGDTGSGKSTLASLVARLRDPSAGTVTIDGIDLRTMGEQDLAAIVGVVS
                     QETYLVHDTIRANLLLAKPAAAEAELWQALAAAQVADLVAGLPQGLDTMVGSRGHRFS
                     GGEKQRLAVARTILRNPKVLVLDEATSALDNKTERALQDALDRLAAGRTTITIAHRLS
                     TIRDADQIAVLHQGRVAEIGTHDQLLARGGRYADLLRASEHPAYARAA"
BASE COUNT          769 a         1842 c         1747 g          808 t
ORIGIN      
        1 cagggggaga ccgtcgcgat cctcggcccg aacggcgccg gcaagaccac caccgcggac
       61 tggctcgcga gaacgagtgg cagggtgcgg cctacctgct cattgcgtgg ggcctggtca
      121 tcaccgtcca atggctcaac ccgaacaccg agacgtggat cctcttcttc gcgctcttcc
      181 cccagacgtg ggccctgctc tcgcgatggc gcgccttctc ggtcaccatc ctggtgacac
      241 tggcgatcgg ggtcgtgcag tggtggcagt ccgaccgtag ctcgggggac gccgccggcg
      301 tcatcgtctc gacgacgctg tcgctcgtgc tgtcactggg actcgggctt ttcatcgcca
      361 ccatggccaa tgaggcgtcc gcccgtgcga agacgatcga cgagctccgc gccgcccagg
      421 cgaaactcgc tgcagcagaa cgcgagagcg gcgtagctca ggagcgagag cggctgtcgc
      481 gcgagatcca cgacaccctg gcccagggat tcaccagtgt gctgaccttg acccgcgccg
      541 cccgggcggc cctcgggcga ggggacgtcg agacggcgat cgcgcggctg gacctcatcg
      601 agcagaccgc cggcgacaac ctgcacgagg cccggctcat ggtcgccgaa ctcaccccgg
      661 ggcacctgca gtcgcgatcc ctggccgagg ccctggagcg gctcgccgcg acagtcagcg
      721 ccgagagtgg actccgtgtc gagatgcaga cccgtggcat gcccatcgcg ctcggcggcg
      781 ccacggacgt ggtgctgctg cgcacggccc aggaggcgat caccaatgcc cgccggcact
      841 cgggggcccg gcacgtgacc atgcagctgg tttacgggga ggacgtccgg ttgatcgtca
      901 ccgatgacgg ccgcggtttc gacacctcga ccccgaggcg cggtttcggt ctggacggca
      961 tccagtcccg ggcgcaggcg atcggtggga cggtcgaggt gcgcagcgcc tccgaccacg
     1021 gaacaaccat cgacgttgct cttcccgctg agccggaccc cgttgcggcc gagcccgatc
     1081 cggggagaac cggcccgccg ccttcgttgt cgaggagtga gtcatgacga cacctgcggt
     1141 gacgcgcatc cggctcgtcg tcgccgacga ccatcccgtc gttcgggcgg gcctggtggc
     1201 gctgctgtcg gaggagccgg acttcgaggt ggtcggccag gcggccgacg gcgccgcggc
     1261 catcgtcgcc gtcaccgaac atcatcccga tctggtgctc atggacctgc ggatgcccgt
     1321 gctggacggc gcggcagcgg ccgcggcgat caccgccctc cccgacgggc cgccggtcct
     1381 catcctgacg acctatgaca ccgatgcgga catcgtgcgg gcggttgaag caggagcgcg
     1441 gggctatctg ctcaaagatg ctccgccaga ggtgctttcg gcagcggtgc ggtcagcggc
     1501 gcgcggcgag acggtgctcg cgccggccat cgcgggccgg ctggccgagc gactgcgcaa
     1561 gccagcagct gcggatctca ccacccgcga gctcgacgtc ctgcggctgg tcgccgaagg
     1621 actgagcaat gccgagatcg ggagtcgcct cttcatcggc gaggcgaccg tcaagacgca
     1681 cctgatccgg gccttccaga agctcgaggt agccgatcgc acggctgccg tgacggaggc
     1741 ctatcggcgc ggtctgctca cgatcgagtg aagggccagc cgtcgcgcat gcccggaccg
     1801 agggccggct cgatgtagta ctcggtgccg aacgccgccg tgaacgcctc ctcgggcatc
     1861 gagagcaggc tctcgatgtc ggtcttctgg ctggcatggg cggcaagggc ggcccgcttc
     1921 tgcggcagga acttgtggac gtcgacagcc caggtgatct cgtattcgtt gctgccgagc
     1981 gggtttccgt catccatggg ctggtcgggg tcccacgcct gctcttcggt ggccccggca
     2041 gcgaccgctt cctggtagag ccggcgcgtc acgtctcgat tcatcgtcga ctcgagcacg
     2101 cggggcggcc gcgcggcgag cttggccgct gcatgcagga cgtggtggac cttgacgtga
     2161 tccggatggc cgtagccgcc atgccagtca taaccgacga tcacgtccgc gtcctcgcga
     2221 tcgaggatct gtgccagccg catgccggcg tcggtcaacg tcgcagtgtg gaaggccccg
     2281 acatgggcgt tctgatccca cccggtcatc ccggaatcgc tgtagccgag ccactcgatg
     2341 cgggctgtgc ccagagctgc ggcggacttc gtcgcctcct tgcggcggcg ttcgacgagc
     2401 gtctcgccgg gcagcagatc gtccggcacc tcgccgtgct caccattggt cgcgtaaacc
     2461 acgacgaccc gatcgccttg tgccgcagcg cgagccatag acccggaggt ctgcgatgcc
     2521 tcgtcgtcgg ggtgggcatg gaagaagacg atggtggcca cgcgcctatt ctccttggtc
     2581 gaggagttcg gtcagtatgc ggtgcaaact cgcccggtcg ttcgcgctca gcctgccgaa
     2641 gaaggcctcc gcctgctcgc gccgcatccg ccccacctcg gtggcgacgg agcgtccgtg
     2701 tccggtcatc tccagcaggg tggcccgccg gtcgctcgga tcgggggagc gcaggaccag
     2761 gccgcgctcc tgcagcgcgt cggcgacctc ggtggccgaa cgcggcacga tccgtagccg
     2821 gcgtgccagc tcggacaggc gcagcggccc gtcctcgtgg acgatccgca gggcccgggc
     2881 ctggtgcggc gcgaggtcca cctcaccgac tcccgctccc cactggtggc gcaactggcg
     2941 gagcgcgcga cccagcagca catccagggg ttcgtcggcg accggcatac cgaacagcat
     3001 acggcgattt gcgtggcagc cacaaagtga ggtaacctct acaactagtg agggaacctc
     3061 acgacatacc tccccgaaag gagggcctct tgaacgagga cgtcaacctt ggtggccgtg
     3121 gcgggggccg cggcccgcag aaactcgacc cggcggatcg agcgcaactg gcgctctccc
     3181 cggtcgaccc tcgacgcatc ctggcgctct tcgcgccgca ccggctcgcc ctcgcgatcg
     3241 tcgtggcgat catcgtcgcc tcctcggcca tcgccttggc gcagcccttc ctcgtcaagg
     3301 ccgtcatcga cgacgccttg ccgcaccaga acgtccgtct cctcctgctc gccgtcggcg
     3361 gcatgctcgc cgtcaccgtg acgacggcgc tgctcggcgt cgcgcagaca tggatctcca
     3421 cgcgcgtcgg acaggaggtc atgcaccgcc tgcgcaccga cgtcttcgcg cacctgcaga
     3481 agcagtcgct cggcttcttc acccgcaccc gcggtggcga ggtccagtcg cgcatcatcc
     3541 aggacatcgg cggtatgcag tccgtggtca cctcgacggc gacctccatc gcctccaacc
     3601 tgaccacggc ggtcgggacg gccatcgcga tggccgcctt gtcgtggcgg ctctcactgt
     3661 tgtcgctgat cgtcatgccg ccggccatct ggctgacccg gcgagtcgcc ttgctgcgca
     3721 aggagatcac cgcgcagctg cagcgccgcc tggccgacat gcacgtccag gtcgacgagg
     3781 gcctgtcggt ctccggcgtc ctgctgacca agaccctcgg cgccggcgcg caggccaccg
     3841 agcgcttcac atcgacgtcc accgaactcg tggacctgga gctgcgcgcg cagctcgccg
     3901 ggcgctggcg gatggcgacg atggcaatca tcttcgcgat catccccgcc ctgctctacc
     3961 tcgccgccgg cttcccggcg acgagcggcg gtatgacgat cggcaccctg gtcgccttca
     4021 ccgccctgca ggccggtctc ttccgtccgg tcatggggct gctcaacgtg ggcgtgcagt
     4081 gggtcagctc gatggcgctc ttcagccgga tcttcggcta tctcgacctg gtcaccgacg
     4141 tccctgctcc gatcgccccg gtggcggtcg accaccaccg cgtgcgcggg gacgttcgtc
     4201 tcgacggcgt gagcttcgcc tacgaggccg gtggcacgcc cgtcctccac gagatcaccc
     4261 tgtcggtgcc ggccggctcc agcctcgcgc tcgtgggcga caccggctca ggcaagtcca
     4321 ccctggcctc actggtggcc cggctccggg atccttccgc cggcacggtg acgatcgacg
     4381 ggatcgacct gcgcacgatg ggcgagcagg acctcgcggc catcgtcggt gtcgtcagcc
     4441 aggagaccta cctggtgcac gacacgatca gggcgaacct gctgctcgcc aagccggccg
     4501 ccgcagaggc cgaactatgg caggcgctcg ccgctgccca ggtcgcggac ctggtcgccg
     4561 gcctgccgca gggcctcgac accatggtcg gctcacgcgg ccaccggttc tccggtggcg
     4621 agaagcagcg cctggccgtg gctcgcacca tcctgcgcaa ccccaaggtg ctggtgctgg
     4681 acgaggcgac ctcggccctg gacaacaaga ccgagcgcgc cctgcaggac gcgctggacc
     4741 ggctcgccgc cggacgcacg acgatcacca tcgcccaccg gctctcgacg atccgcgacg
     4801 ccgaccagat cgcggtgctc caccagggtc gggtggccga gatcggcacc cacgaccagc
     4861 tgctcgcccg cggcggtcgg tatgccgacc tgttgcgtgc gagcgagcac ccggcatacg
     4921 cccgggccgc ctgaggcgcg ctcaccgaca acccgcggca gcgtcgaact ccgggcggag
     4981 agatgtcggt gcgcatcgca acatttccga tgcgtaccga cacaaagtgg cactgggcgg
     5041 aaaagtgcag tggcggccgc ggctcaggcg ttcaggccgg ctttggcttc gcggcggtgt
     5101 ttgaggtatt cgaggaccat cgggatcacc gagacggcga cgatcagcag gatcgcggcc
     5161 tcgagg
//
LOCUS       DAOPJG010000217         5059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_17928_length_5059_cov_8.851119, whole genome shotgun sequence.
ACCESSION   DAOPJG010000217 DAOPJG010000000
VERSION     DAOPJG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17928_length_5059_cov_8.851119"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PKU75_14960"
     CDS             <1..875
                     /locus_tag="PKU75_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82225.1"
                     /translation="PSTKGYITITFGRSNWSVATGATCNVIPSGARTLEQNVQDLAAR
                     GLMAQGGVVVDRTHATARECTEGQMLQPSWADLTRLRVNNGFTVVSQGMTYADMTKMT
                     TDAQRYNESGATLPIFNSRGHTRAWGMFNYPNNKMDAAANRVVTKYFAFGRKYGTEIN
                     NRAAVMTYPYLVKTISVMGGRCNNVNLACYTMSVKNNKQMAPPERLSRILRPGADQWG
                     VVQFYRIVEGSYGRMGASVAWDCTSSDWRNRWTSQPEIYCRNSFLEAIDGRSKTAVNA
                     DAVDVAKAWGRRPG"
     gene            complement(965..2218)
                     /locus_tag="PKU75_14965"
     CDS             complement(965..2218)
                     /locus_tag="PKU75_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HPF82226.1"
                     /translation="MSFLEPSPADEARRAGLRRMRIVATSLLAVAFVIFVLTNGRDHG
                     VWGYVHTASEAAMVGALADWFAVTALFKHPLGVPIPHTALVKKRKDELGRSLEEFVSD
                     NFLTEEIARERLAAAQVGVRLGTWLETPAHRERALTEMARIAKAGLGRMRDQDVHDFV
                     VGVLLPRLQQEPVSPIAGSFLKGIVEDKAHYQLVDLGLTEIHDWLAANPKTFTGLMNE
                     RVPWWTPPWVDDRVINWTYQQALAWLQDMRADPDHRAREALDNLLTKLAEDLHDNPEV
                     QERAEALKERVLSHPQVGETVLSLWASVRTSLLAALEDPSSTLWQRGDAWLTEASSML
                     LHDDATRERLEAHLGDIVAFFVTTYGDELANVISHTVDRWNADEASERIELFVGRDLQ
                     FIRINGTIVGALAGVIIHAIAQLLR"
     gene            2324..3142
                     /locus_tag="PKU75_14970"
     CDS             2324..3142
                     /locus_tag="PKU75_14970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPF82227.1"
                     /translation="MNAPADELAAHTALWSRACDDLDSLLGELSETDWAAATDCPGWS
                     VQDVVAHLASIEHALNTGEQPSGIVPVEGRSLASSWTEAGVAERRGMPHTQVVEQWRA
                     EVAARRAHLAAQPPTDPRAPAVNAPGGVPWDNRTLLRNRVLDVWVHEQDIRRAVGRPG
                     GMDSPAGELVAGIFAAAVPYAVARRAAAPAGTRVAVVVDGERRDVLVDDAGHAAFATT
                     QDPADVTLDLNREALTVLGAGRRAPDDLPPTAYAAIAGDPDLADRILRGLNVTP"
     gene            3276..4712
                     /locus_tag="PKU75_14975"
     CDS             3276..4712
                     /locus_tag="PKU75_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HPF82228.1"
                     /translation="MRFLPGPAPAYDLTYDDVFLVPSRSAVTSRFDADLATPDGSGTT
                     IPLVVANMTAIAGRRMAETVARRGGLVVIPQDIPLDVVAEVISWQKSRHLLYDTPITL
                     SADQTVGEAIVLLTKRAHGAAVVVEDDRPVGLVTEADLTGVDRFSQVGSVMSTQLATL
                     PDGVSPEAAFDTLASVRHRLAPVVRPDGTLVGLLTRQGALRATVYRPNTDAHGRLRIA
                     AAIGINGDIPGKARALAEAGADVLVMDTAHGHQEKMIEALTAVRALDLGVPIAAGNVV
                     TADGTRDLIAAGADIVKVGVGPGAMCTTRMMTAVGRPQFSAVLECATAARELGKHVWA
                     DGGIRHPRDVALALAAGASNAMIGSWFAGTYESPGDIFTDETGRSYKESFGMASARAV
                     ANRTSGESSFDRARKALYEEGISHARMFIDPARPSVEDLIDSVTAGVRSSFTYAGAAT
                     VEEFGERAVVGVQSLAGFAEGRPLHTSW"
     gene            complement(4736..>5059)
                     /locus_tag="PKU75_14980"
     CDS             complement(4736..>5059)
                     /locus_tag="PKU75_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF82229.1"
                     /translation="PDEPLVTSAGDEAAIGSAGTGLPPHRLASVNALFVDPTAAGQGV
                     GAALHDVAVAAIREEGLVPVLDVVSRNTAAVRLYERRGWRLIGTGRPDWLPDEWDPVW
                     YMTLD"
BASE COUNT          771 a         1754 c         1803 g          731 t
ORIGIN      
        1 cgccgtcgac caagggctac atcaccatca cgttcgggcg cagcaactgg tcggtcgcga
       61 cgggggccac ctgcaatgtc atccccagcg gcgcgcgcac actcgagcag aacgtccagg
      121 acctcgccgc gcgcggcctg atggcccaag gaggcgtcgt cgtcgatcgc acccacgcca
      181 cggcgcggga atgcaccgag gggcagatgc tgcagccgtc gtgggccgac ctgacgcggc
      241 tgcgcgtcaa caacggcttc acggtcgtca gccagggcat gacctacgcc gacatgacga
      301 agatgaccac ggacgcccag cgctacaacg agagtggcgc gaccttgccg atcttcaaca
      361 gccgcggcca tacccgcgcc tggggcatgt tcaactaccc caacaacaag atggacgcgg
      421 ccgccaaccg ggtggtgacc aagtacttcg cgttcggccg caagtacggc accgagatca
      481 acaaccgcgc cgccgtgatg acctacccct acctggtcaa gaccatttcc gtcatgggcg
      541 ggcgctgcaa caacgtcaac ctcgcgtgct acacgatgtc ggtgaagaac aacaagcaga
      601 tggcgccgcc ggagcggctc tcgcggatcc tgcgcccggg cgccgatcag tggggtgtcg
      661 tgcagttcta tcgcatcgtc gaggggtcgt atggccgtat gggtgcctcc gtcgcctggg
      721 actgcacctc cagcgactgg cgcaaccggt ggaccagcca gccggagatc tactgccgca
      781 acagcttcct cgaggcgatc gacggccggt ccaagacggc cgtgaacgcc gacgcggtcg
      841 acgtggccaa ggcttggggc cgcaggccgg gctgaggcct gctgccagcg ccggctgcgc
      901 gtccggtgcc ccgccgagac cggcgagcct gagccttcgt ggtgtcagga ccttcgtggc
      961 gtggtcagcg cagcagctgg gcgatggcgt ggatgatcac gcccgccagc gcgccgacga
     1021 tcgtgccgtt gatccggatg aactgcaggt cgcgaccgac gaacagttcg atccgctctg
     1081 aggcctcgtc ggcgttccag cggtcgaccg tgtgggagat gacattggcg agttcgtccc
     1141 cgtaggtggt gacgaagaag gcgacgatgt cgccgagatg ggcttcgagg cgctcgcggg
     1201 tcgcgtcgtc gtggagcagc atcgagctgg cctcggtcag ccaggcgtcg ccgcgctgcc
     1261 acagcgtgct cgacggatcc tcgagggcgg cgagcaggct ggtgcgcacg ctggcccaca
     1321 acgacaggac ggtctcgccc acctgcgggt gggacagcac gcgctccttg agcgcctcgg
     1381 cccgctcctg gacctcgggg ttgtcgtgca ggtcctcggc gagcttggtc agcaggttgt
     1441 ccagcgcctc ccgggcccgg tggtcggggt cggcgcgcat gtcctgcaac caggccaggg
     1501 cctgctggta ggtccagttg atcacccggt cgtccaccca cggcggagtc caccacggca
     1561 cgcgctcgtt catcaggccg gtgaaggtct tgggattggc cgcgagccag tcgtggatct
     1621 cggtcaggcc gaggtcgacc agttggtaat gcgccttgtc ctcgacgatc cccttgagga
     1681 aggagccggc gatcgggctg accggttcct gctgcaggcg cgggagcagc actcccacga
     1741 cgaagtcgtg aacgtcctga tcacgcatcc gccccagccc ggccttggcg atgcgggcca
     1801 tctcggtcag cgcgcgctcg cggtgagccg gcgtctccag ccaggtgccc agccggacgc
     1861 cgacctgggc ggccgccagc cgctcccggg cgatctcctc ggtgaggaag ttgtcgctga
     1921 cgaactcctc caggctgcgt cccagctcgt ccttgcgttt cttgaccagc gccgtgtgcg
     1981 ggatcggcac cccgagcggg tgcttgaaga gcgccgtgac ggcgaaccag tcggcgagcg
     2041 cgccgaccat ggcggcctcg gaggcggtat gcacgtagcc ccagaccccg tgatcgcgcc
     2101 cgttggtcag gacgaagatc acgaacgcga cggccagcaa cgacgtcgcg acgatccgca
     2161 tccggcgcag gccggcccgg cgtgcctcat cggctgggct cggctccaag aaactcacgt
     2221 actcatcctt cccgatggca cccccgcatg ccgtagggga tacccccgat tgccaactcc
     2281 cggggagccc gcaggattcc gggtcgcatg cttggatggc gccatgaacg ctcccgccga
     2341 cgaactcgcc gcccacaccg ccctgtggtc acgcgcctgc gacgacctcg actccctgct
     2401 gggtgagctc tcggagacgg actgggcggc ggcaaccgac tgcccggggt ggagcgtgca
     2461 ggatgtcgtc gcgcacctgg cctcgatcga gcacgccctc aacaccggcg agcagccgag
     2521 cggcatcgtg cccgtcgagg gccgctcgct cgcgtcctcg tggaccgagg ccggggtggc
     2581 ggagcggcgc ggtatgccgc atacccaggt cgtggagcag tggcgcgccg aagtcgcggc
     2641 gcggcgcgcg cacctggccg cgcagccgcc gaccgacccg agggcaccgg cggtcaacgc
     2701 gcccggcggg gtgccgtggg acaaccggac gctgctgcgc aaccgggtgc tcgacgtgtg
     2761 ggtgcacgag caggacatcc ggcgggctgt cggccgcccg ggcggtatgg acagtcccgc
     2821 cggcgagctg gtcgcaggga tcttcgccgc cgcggtgccg tatgccgtgg ccaggcgtgc
     2881 cgctgccccc gccgggaccc gcgtggcggt ggtggtcgac ggcgagcgcc gtgatgtcct
     2941 ggtcgacgac gccgggcatg cggcgttcgc gacgacacag gacccggccg acgtcactct
     3001 cgacctgaac cgggaggccc tcaccgtcct cggcgccggc cggcgcgccc cggacgacct
     3061 gccgcccacg gcatacgcag caatcgccgg cgaccccgac ctcgccgacc gcatcctgcg
     3121 cggcctcaac gtcaccccct gacccgacga gacactgacc cgacgagaca ctgacccgtc
     3181 gagacagcga tttcacgggc gtcgagatcg cgcttcgtca cacgcgtcat acggctgcag
     3241 cgccgaggcc ggcgcaggga tgaaaagcta gggggatgcg tttcctgcct gggcccgcgc
     3301 ccgcgtacga cctgacgtat gacgatgtct tcctcgtccc gagccgctcg gcggtgacga
     3361 gccggttcga cgccgacctg gccacgccgg acgggtcggg gacgacgatc ccgctcgtcg
     3421 tggcgaatat gacggcgatc gcggggcggc ggatggccga gacggtggcc cgccgcggcg
     3481 gactggtcgt catcccgcag gacatcccgc tcgacgtcgt ggcggaggtc atctcgtggc
     3541 agaagtcgcg gcacctgctc tatgacacgc cgatcacctt gtcggcggac cagacggtgg
     3601 gcgaggcgat cgtcctgttg accaagcgtg ctcacggggc ggcggtcgtc gtcgaggacg
     3661 accgtccggt gggcctggtg accgaggccg acctgacggg ggtggaccgc ttctcgcaag
     3721 tgggcagcgt gatgtcgacc caactcgcga cgctgcccga cggagtctcc cccgaggcgg
     3781 ccttcgacac cctcgcctcg gtgcgtcacc ggctggcgcc cgtggtgcgg cccgacggga
     3841 cgctggtcgg tctcctgacg cggcaggggg cgttgcgggc aacggtctat cggcccaaca
     3901 ccgatgccca cggccggctg cggatcgccg cggccatcgg catcaacggc gacatccccg
     3961 gcaaggcgcg agcgctggcc gaggcgggcg cggacgtgct ggtcatggac accgcgcatg
     4021 ggcaccagga gaagatgatc gaagccctca ccgcggtgcg ggcgctcgac ctgggcgtgc
     4081 cgatcgcggc gggcaatgtc gtcacggccg acgggacccg ggacctgatc gcggcgggcg
     4141 ccgacatcgt caaggttggg gtcggtccgg gggcgatgtg cacgacccgg atgatgactg
     4201 ccgtcggacg tccgcagttc tcagccgtgc tcgaatgcgc tacggccgca agggaactcg
     4261 ggaagcacgt gtgggcggac ggcggcatac ggcacccgag ggatgtggcg ctggcgctcg
     4321 ccgcgggagc gtctaacgcg atgatcggct cgtggttcgc gggcacctac gagtcgccgg
     4381 gcgacatctt caccgacgag accggccgct catacaagga gtctttcggc atggcctcgg
     4441 cgcgcgccgt cgccaaccgc acgagcggcg agtcctcctt cgaccgggcg cgcaaggcgt
     4501 tgtacgagga gggcataagc cacgcgcgga tgttcatcga ccccgcccgg ccgagcgtgg
     4561 aggacctgat cgactcggtc accgcggggg tgcgctcctc gttcacgtat gccggcgcgg
     4621 ccaccgtgga ggagttcggc gagcgcgcgg tcgtgggcgt gcagtcgctg gccggcttcg
     4681 cggaggggcg gcccctgcat acctcgtggt gacgggcaca cggtcgtgac gactgtcagt
     4741 ccaaggtcat gtaccagacc gggtcccatt cgtcggggag ccagtcggga cgtccggtgc
     4801 cgatcagccg ccagccgcgg cgctcataga gccggacggc cgcggtgttg cggctgacga
     4861 cgtcgaggac cggcaccaat ccctcctccc tgatggcggc gaccgcgaca tcgtgcaggg
     4921 cggcccccac gccctgaccg gcggccgtgg ggtcgacgaa cagggcgttc acgctggcca
     4981 gccgatgagg cggtagcccc gtgccggcgc tcccgatggc cgcctcgtct ccggccgagg
     5041 tgacgagcgg ctcatccgg
//
LOCUS       DAOPJG010000218         5034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_18052_length_5034_cov_8.301064, whole genome shotgun sequence.
ACCESSION   DAOPJG010000218 DAOPJG010000000
VERSION     DAOPJG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18052_length_5034_cov_8.301064"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1058)
                     /locus_tag="PKU75_14985"
     CDS             complement(12..1058)
                     /locus_tag="PKU75_14985"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HPF82230.1"
                     /translation="MSTDTPDTDGRTGVLPAVVASDPGTMAHNGLHGPLRGTPERVAG
                     SIRRTTNLDLIRPEGPDGPIRITGRGRDLRTAADGRGIVRATALIAATVEGDRVTALT
                     THPRPPGIADMVGRRAVVGWRTGLWRHLRGQHDAGTVLHLVLDDLPGGMIIGGFTRRR
                     AIAESAGGPLPQPGRRLDVCAGWAAESRAAITLAQTGIPPAPVTPLAPDLGCDTDSLA
                     WHTLPELPAYGISRRRRIDVLDTGEEVVAEAWFRDSFTDADGAHRVLHEYRVSVTADR
                     GSYAVRHLSAHPRVLPHRECPVAAASAQRMRGIPLGQLRERVSSDLFGPESCTHLNDL
                     LRSLADVPGLLALG"
     gene            1164..3173
                     /locus_tag="PKU75_14990"
     CDS             1164..3173
                     /locus_tag="PKU75_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HPF82231.1"
                     /translation="MNISLGGPESDYDRQATLLGHTFRIIRVGTSGDVEAAIDVARTW
                     SERAAAFAFSGIRETPGGALLDITDADVAALRRACRRVPLTDGHRLADVLEEWSIRAV
                     QTEYPAHFTNARTIVLGGGYKRAVAVLASFTRNIEYVPVKGERPNPVIQAAKSVKGLA
                     DEFAGKAKEAVRDPLAPALWAMGKIAQPSHKGQDVVVGLFDDIVSAGPSELSGITVIS
                     SAISPEREAELAERDVDMIVDTTPQPFRNVTVVTAVLEAMMLHHRGKRKLDVESMLTA
                     IEEAGLQPRMLHPNGPRRKSRFAFVIHPLSTQYFRNVEPLGSLTKVPGMTPVIEKAAA
                     YFPPFVYGRMTGITSPTGAEAEGWLISVGGTPKELLAHSPEFTYDRLLEAAEIAQGLG
                     AQVMGLGAFTKVVGDAGVTVAQRAPLPVTTGNSYSASGALWAAHEAIRRLGIAEIDAD
                     GRLRGKAMVVGATGAIGSVCARLLAMACDELWLVSPETAKLLTIKNSIEREHPRASVH
                     VAAKPDKHLSEMDMIVTATSAAGQKVLDVMRIKPGCVVTDVARPLDLSADEVAKRPDI
                     LVIESGEIELPGDVHMRDIGLPKGVAYACLAETVVLALEGRYERFTVGREIEWAKVKE
                     IYQLGLKHGMKLATISGVNGVFTEADFARVRELALAARAAQEAKK"
     gene            3170..4000
                     /locus_tag="PKU75_14995"
     CDS             3170..4000
                     /locus_tag="PKU75_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF82232.1"
                     /translation="MKDVAGATVLITGAAMGMGRLFAERAIAEGADTVVLVDVNAGAL
                     EQTAAELSGGATRVLAEVVDVRKLTDIRACAKRVANTAGPVQILINNAGVVRGNHYFW
                     ETDPVADTKFTMEVNALAPMYVARVFLPQMIESDADCRLVNLASAAGLTANPRMAVYA
                     ASKWAVVGWSDSVRLELEQAGHHHVKVTTVCPYYVKTGMFDGARSAPLLPLLEPQDVV
                     DSTWHAMVRGDAMLVMPRTVILSETVKGILPVGVRDFVADKVLGIYHTMDDFTGRNPG
                     "
     gene            4128..4874
                     /locus_tag="PKU75_15000"
     CDS             4128..4874
                     /locus_tag="PKU75_15000"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPF82233.1"
                     /translation="MSASTAVSVPRTVGAKQRVAAALGEASAVTALLAVFTWIQNRLG
                     TNTAAAHANADAVARLDGSEILGWEVSANTWLAGIPWLAALSALLYSVVLFAPPAVLA
                     WAWWRDRAAYGRLRTMLVTLTFASLIPFALFPVAPPRLHVPGTVDIVERYRLLGSSTT
                     PTTESAANLFAATPSLHVAWACWVAYAVWVVLRRRGFRPYAVWSFPVVAAADVVVTAN
                     HYLLDVFSGGLLALLTIAGVGAGHRWLARI"
BASE COUNT          793 a         1693 c         1729 g          819 t
ORIGIN      
        1 gaccgagagc gtcagccgag ggcgaggagg ccaggcacgt cggccagtga gcgcagcagg
       61 tcgttgaggt gcgtgcacga ctccgggccg aaaagatctg acgaaacccg ttctcgcagt
      121 tgaccaagcg gtatgccgcg catccgctgt gccgaggctg cggcaacggg gcattcgcga
      181 tggggcagga cgcgcgggtg cgcggagagg tggcgcacgg catacgaccc tcggtcggcg
      241 gtgaccgaaa cgcggtattc gtggagcacg cggtgtgcgc cgtccgcgtc ggtgaacgaa
      301 tcacggaacc aggcctcggc gacgacttcc tccccggtgt cgaggacatc gatccgccgt
      361 cggcgggaga tgccgtatgc cgggagctcc ggaagcgtat gccacgccag ggaatccgta
      421 tcgcagccga ggtcaggggc gagcggagtg accggagcgg gaggtatgcc ggtctgcgcc
      481 aacgtgattg ccgcgcggga ttcggcggcc cagccggcgc acacgtcaag tcgccgaccc
      541 ggttgcggca gtgggccacc ggcggattcg gcgatcgccc gacgacgggt gaagcccccg
      601 atgatcatgc cgccgggcag atcgtccagc accaggtgca acacggtgcc ggcgtcatgc
      661 tgcccgcgca ggtgacgcca cagtccggtg cgccacccga caaccgcacg ccggcccacc
      721 atgtcggcta tccccggggg acgtggatgg gtcgtcagcg cggtgacacg gtcgccctcg
      781 acggtcgcgg cgatcagagc ggtagcgcgg acgatgcccc ggccgtcggc agccgtccgc
      841 aggtcccgtc cccggccggt gatccggatg ggaccatcgg gtccctccgg gcgaatcagg
      901 tccagattcg tcgtcctccg gatcgatccg gcgacgcgtt ccggggtgcc cctcagcggt
      961 ccgtgcagtc cgttgtgagc catggtgccc gggtcactcg cgaccaccgc gggtagcacg
     1021 ccggtgcggc cgtcagtgtc gggtgtatcg gtgctcacac cgacatcctc gcgcacgctc
     1081 agcaagcctc ggtcaggctg gttgccattg gcttatggcc gcccattggt gggaggctta
     1141 ggggcatgac tcgacccgtg atagtgaaca tcagtcttgg cgggccggag tccgactacg
     1201 accggcaggc caccctgctc gggcacacct tccgcatcat ccgggtcggg acgtcgggtg
     1261 acgtcgaagc ggcgatcgac gtggcgcgca cctggtcgga gcgggccgcc gccttcgcct
     1321 tctccggcat ccgcgaaacc cctggtggag ccctcctcga tatcaccgac gctgacgttg
     1381 cggccctgcg ccgcgcctgc cgtcgcgtcc cgctcacgga tggccaccgg ctggcagatg
     1441 tgttggagga gtggagtatt cgcgccgtac agaccgagta ccccgcccac ttcaccaacg
     1501 cgcgcacgat tgttctcggt ggcggataca agcgggccgt cgcggtgctg gctagcttca
     1561 cccgcaacat cgaatatgtg cccgtcaagg gggagcggcc caatccggtc atccaggcgg
     1621 cgaagtccgt gaagggcctg gccgacgagt tcgcgggcaa ggccaaggaa gccgtccgcg
     1681 atccgctcgc gccggcgctg tgggcgatgg gcaagatcgc ccaaccgtcc cacaagggcc
     1741 aggacgtcgt cgtcgggctc ttcgacgaca tcgtctccgc cggtccgtcg gagttgtccg
     1801 ggatcaccgt gatctcctcg gccatctcac ccgagcgcga ggccgaactc gccgaacgcg
     1861 atgtcgacat gatcgtcgac accactccgc agcccttccg caatgtcacg gtcgtgaccg
     1921 ccgtccttga ggcgatgatg ttgcaccacc gcggcaagcg gaagctggac gtcgagtcga
     1981 tgctcaccgc catcgaggag gccgggctgc aaccgcgcat gttgcacccc aacggccctc
     2041 ggcgcaagag ccggttcgcg ttcgtcatcc accccctctc gacccagtac ttccgcaacg
     2101 tcgagcccct cggttcgctg accaaggtgc ccggcatgac cccggtcatc gagaaggccg
     2161 ccgcctactt cccacccttc gtttacggac ggatgaccgg catcacctcg ccgacgggtg
     2221 ccgaggccga agggtggctg atctctgtcg gcggcacacc caaggagttg ctggcgcaca
     2281 gtcccgagtt cacctacgac cggctcctcg aagcggccga gatcgcgcag ggcctcggcg
     2341 cccaggtgat ggggctcggt gccttcacca aggtcgtggg cgatgcgggt gtcacggtgg
     2401 cacaacgggc tccgctgccg gtgacgacgg gcaacagtta cagcgcgtcc ggtgccctgt
     2461 gggcggcgca cgaggccatc cggcgcctcg gcatagccga gatcgacgcc gacgggcggt
     2521 tgcgtggcaa ggcgatggtc gtcggcgcca cgggcgccat cggctcggtg tgcgcgcggc
     2581 tgctcgcgat ggcgtgcgac gaactgtggc tcgtgtcacc ggagacggcc aagctgctca
     2641 cgatcaagaa ctcgatcgag cgggagcacc cgcgcgcgag tgtgcacgtg gccgccaagc
     2701 ccgacaagca cctgtccgag atggacatga tcgtcacggc cacgtcggcg gccggtcaga
     2761 aggtcctcga cgtcatgcgc atcaagcccg gctgtgtcgt caccgatgtc gctcgcccgc
     2821 ttgacctttc ggccgatgag gtcgccaagc gccccgacat tctcgtcatc gaatccggag
     2881 agatcgagct gcccggcgat gtgcacatgc gcgacatcgg tctacccaag ggcgtcgcgt
     2941 atgcctgtct cgccgagacc gtcgtgctcg ccctcgaggg ccggtacgag cggttcaccg
     3001 tcggccggga gatcgagtgg gccaaggtca aggagatcta tcaactcggg ctcaagcacg
     3061 ggatgaagct cgccacgatc tccggagtca acggcgtctt caccgaggcc gacttcgccc
     3121 gcgtccgcga actcgccctg gctgcccgcg ctgctcagga ggccaagaaa tgaaggatgt
     3181 cgccggtgcc acggtcctga tcacgggcgc tgcgatgggg atgggacgcc tcttcgccga
     3241 gcgcgccatc gccgagggag ccgacacggt ggtgctcgtc gacgtcaacg ccggcgccct
     3301 cgaacagacg gcggccgaat tgtccggcgg tgccacccgg gttctcgccg aggtggtcga
     3361 cgtgcgcaag ctgaccgaca tccgggcgtg cgcgaagcgc gtcgccaaca ccgccggccc
     3421 ggtccagatc ctcatcaaca acgccggtgt cgtgcggggc aaccactact tctgggaaac
     3481 cgacccggtg gcggacacca agttcacgat ggaggtcaac gcgctcgctc cgatgtatgt
     3541 cgcgcgggtg ttcttgccgc agatgatcga gagcgacgcc gactgccgcc tggtcaacct
     3601 cgcctccgcc gccggcctga ccgccaatcc ccggatggcc gtgtacgccg cctccaagtg
     3661 ggccgtcgtc ggctggtccg actcggtgcg gctggagttg gagcaggccg gacaccacca
     3721 cgtgaaggtc acgacggtct gcccctacta cgtcaagacc ggcatgttcg acggggcccg
     3781 gtcggcgccg ctgttgccgt tgctcgaacc ccaggacgtc gtcgacagca cctggcatgc
     3841 gatggtgcgc ggggacgcca tgctggtcat gccgcggacg gtgatcctca gcgagacggt
     3901 caaggggatt ctgcctgtgg gagtaaggga tttcgtcgcc gacaaggtgc tcgggatcta
     3961 tcacacgatg gacgacttca ccgggcgtaa ccctggttga tcccccatca tcggtggggg
     4021 tccttgacgc gggtcgaggt tcgcttggca cacttccttg catgacgtcc gtgaggacag
     4081 ctggggggag ttgacgagtg agcgcgacga caacgtccgt gacaccggtg agtgcgtcca
     4141 cggccgtgag tgtgcccagg accgtgggag ccaagcagcg ggtggccgcg gcactcgggg
     4201 aggcgagtgc ggtgactgcg ctgcttgcgg tctttacgtg gatccagaac cggctgggca
     4261 ctaataccgc tgcggcgcac gcgaatgctg acgctgtcgc ccgcctggac ggaagcgaga
     4321 tcctggggtg ggaggtcagt gcgaacacgt ggctcgccgg gatcccttgg ctcgcagcgc
     4381 tttcggcgct gctctacagc gtcgttctct tcgcgccacc ggcggtcctc gcgtgggcct
     4441 ggtggcggga ccgcgcggca tacggccggc tgcgcaccat gctcgtgacg ttgacgttcg
     4501 ccagcctcat cccgttcgcg ctctttcccg tggccccgcc gcggctgcac gtgccgggca
     4561 ccgtcgacat cgtcgagcgc tatcggctgc tcggttcgtc gaccacgccg acgaccgaga
     4621 gtgcggccaa cctcttcgcc gctaccccga gtctccatgt cgcctgggcg tgctgggtcg
     4681 cctacgcggt gtgggtggtg ctgcggcgcc gcggcttccg gccgtatgcc gtctggtcct
     4741 tccccgtcgt cgcggcggct gatgtcgtgg tcaccgcgaa tcactatctc ctcgacgtct
     4801 tttcgggcgg gctgctggct ctgctcacca tcgccggcgt cggcgccggc caccggtggc
     4861 tggcccgcat ctgaggtagc gcagcgggca aaatagggcg tcctgccgag gtgcgggact
     4921 acctcagacg gcgagtctcg tggtgtcgac aacgacgaaa acccgagaca gggagcagtc
     4981 atgagctaca tcacccccga actggtcgag gccgtcaccg cacgccacta cgag
//
LOCUS       DAOPJG010000219         5011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_18160_length_5011_cov_6.216102, whole genome shotgun sequence.
ACCESSION   DAOPJG010000219 DAOPJG010000000
VERSION     DAOPJG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18160_length_5011_cov_6.216102"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1371)
                     /locus_tag="PKU75_15005"
     CDS             complement(169..1371)
                     /locus_tag="PKU75_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPF82234.1"
                     /translation="MRRNTFRRAVTAAIAATTLAFGLTPTLGASATPTTADSPRASRP
                     CSAETRAEYARCMSQFRQENAGRLDSRGTPPGMTAADLRSAYKAPAKGAHTTVAIIIA
                     FDNPNAEKDLAVYRTTMGLPPCTTANGCFTKLNQRGVKGDYPQPDGGWALEGSMDLDM
                     VSAACPTCKIMLVEADDNYMNSMAEATRTATRKGATVLSHSYGGGEGGYMWQLRDAYE
                     KPGTISVASSGDYGFMQASTPAVFRKVLAVGGTTLTKKPGTGRGWDEDVWSGSGSACS
                     AYVMKSPWQKDTHCRMRTVADVSAVADPATGVAVYDTYENPFGLEPGWIIGGGTSASA
                     PYIAGLIGLKGNGSTFTSRTPYDNRSAFFDVVGGANGLCGGDYLCNGLKGYDAPTGVG
                     SPRGLGGM"
     gene            complement(1401..3578)
                     /locus_tag="PKU75_15010"
     CDS             complement(1401..3578)
                     /locus_tag="PKU75_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82235.1"
                     /translation="MKKNLIAAMAGSTVLAAGVGAGTATASSPAPAAGTRPPSRTWTP
                     TAPSSVSTGVAVLPNGDRVGVQRNPGAAPTVTAPADLARAGARSMTIGTTTYVIPRRM
                     SGVAGRVFDKALFDVGTLAARGSGRTAVNVTYASSSAPTAVPGIVITARTGTTATGYV
                     DAASSAALGRALATTDAKTLFAHVRTVAPPAQPAATPRWPMVTVTIRLIGLDGRPLDD
                     LVGFTNVVDPGKGNGMVFTTHGIAKISVPAGTYQLLGSTFTEVAGKPVVAMGAGRETT
                     VGDGDEIVLDLRKATMRLKTSVNKPVVNPFWAQSVNREIQGPTGGVGFSLSALGDDSF
                     SVRVVPTVGTLHGIQSLTKDYLAVSPSGVTPAYRFATHYSIDGRIPDGTIVKHSDASN
                     LMTYKDRFTGVRSLASASFARAVTGHGGGWSLGAPLPGDRLTTYATAGPGLDHSARLD
                     QYISFDPTFTTKGSFASPPAASPKPGTIKSVVWGVGPLHQRYAAPPASAPFKICPACV
                     GDGVFNVEAPPLSTNAQLGSADLVEPDILGRVTIKADDTVLYAGQGQLSGYSALPAGT
                     KSVTTTQVATRSGAVFPRTTVLSTKSSTKMAASFPAPDGWMCLAETGCRVLPFLSVDY
                     RIAGQTALGALPAGKRRLDLTVNQVGRATAKGVTSVKVWVSYDGKTWTAAPVTGSGAQ
                     RAVALTIPEPGSGRTGVNLKVSVADAAGSTYTEQITGAFGLAG"
     gene            complement(3895..5010)
                     /locus_tag="PKU75_15015"
     CDS             complement(3895..5010)
                     /locus_tag="PKU75_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPF82236.1"
                     /translation="MIDLVREITEKELRPRADASEAAAEKSDAFPRDVFRMLGQAGLM
                     SLPYPEEFGGAGQPFEVYLQVLEELSSAWMAAGLGISVHTLTCYPVFTYGTDEQKADL
                     LPDMLSGETLGAYALSETQAGSDIASMTTRARRDGDDYVINGKKAWISHAGHADFYTT
                     FARTSDDGGRGVSCFIVPADTPGLSFGAPERKMGLHGDTVREVIYEDVRVPASRRIGE
                     EGQGVPIALAGLDSGRLGIAAGATGLAQGALDLAALYAVEREQFGRPIASNQGLAFVI
                     ADMAAAIGAARATYVHAARLRDAGRPFSKEAAIAKLVATDAAMKVTTDAVQVLGGAGY
                     TQDFPVERYFREAKVTQIFEGTNQIQRLVISRHVLGR"
BASE COUNT          740 a         1642 c         1813 g          816 t
ORIGIN      
        1 agttgtcggc ggccaaagcc gcccccggaa aggtacggga tcgacccccg gtgtgcaatt
       61 gctgttcacc ggctgtgtcg cgcagatcac cgtatgccgc gagcccagca cccgcgaacg
      121 cgccggtgac cagttgtcac cggcccgccc aacgggtatg ccgagtcgtt acatcccgcc
      181 caacccgcgg ggggagccga cgccggtcgg ggcgtcatac cccttcagcc cgttgcacag
      241 gtagtcgccg ccgcacaggc cgttggcccc gccgacgaca tcgaagaagg cgcttcggtt
      301 gtcgtagggg gtgcggctgg tgaacgtgct gccgttgccc ttcaggccga tgaggccggc
      361 gatgtagggg gcggaggcgc tggtgccacc gccgatgatc cagcccggtt cgagcccgaa
      421 cgggttctca taggtgtcgt agaccgccac cccggtggcg gggtccgcga cggcggagac
      481 gtcggcgacg gtgcgcatcc ggcagtgggt gtccttctgc cacgggctct tcatcacgta
      541 ggccgagcag gcgctgccgc tgccgctcca gacgtcctcg tcccagccgc gcccggtgcc
      601 gggcttcttg gtgagagtcg tgccgccgac ggcgaggacc ttgcggaaca ccgcaggggt
      661 ggaggcttgc atgaacccgt agtcaccgga ggaggccaca gagatcgtcc cgggcttctc
      721 gtaggcgtcc cgcaactgcc acatatagcc gccttcgccc ccgccatagc tgtgggacag
      781 cacggtggcg cccttgcggg tcgcggtgcg agtagcttcg gccatgctgt tcatgtagtt
      841 gtcgtcggcc tcgacgagca tgatcttgca ggtcgggcag gccgccgaga ccatgtcgag
      901 atccatcgac ccctccaggg cccagccgcc gtccggctgc gggtagtcgc ctttgacgcc
      961 gcgctggttc agtttggtga agcagccgtt ggcggtcgtg cacggcggca ggcccatcgt
     1021 cgtgcgatag accgcgaggt ccttctccgc attggggttg tcgaaggcga tgatgatggc
     1081 gaccgtggtg tgtgcgccct tggcgggggc cttgtatgcc gagcggaggt cggccgcagt
     1141 catgccgggc ggggtgcccc tcgagtcgag gcggcccgcg ttctcctgcc ggaactgact
     1201 catgcaccgg gcgtattcgg cgcgtgtctc ggccgaacac ggccggctcg cgcgaggcga
     1261 gtccgctgtg gtcggggtcg cgctggcgcc gagggtgggc gtcaacccga acgccagcgt
     1321 ggtggcggcg atggcggcgg tcacggcccg ccggaaagtg ttgcgacgca tggtttctcg
     1381 ctttctcgtg ccgtccgcga tcagccggcc agcccgaagg cgccggtgat ctgctcggtg
     1441 taggtggaac cggcggcgtc ggcgaccgac accttcaggt tgactccagt gcgcccggac
     1501 ccgggctcgg ggatggtcag cgcaacggcg cgctgggcgc cggacccggt gacgggggca
     1561 gcggtccagg tcttcccgtc atacgacacc cagaccttca cggacgtgac gccctttgcg
     1621 gtggcccggc cgacctggtt gaccgtgagg tcgagtcggc gtttgccggc cggcagcgcg
     1681 ccgagggcgg tctgacccgc gatgcggtag tccacgctga ggaagggcag tacccggcaa
     1741 cccgtctcgg cgaggcacat ccacccgtcg ggtgccggga aggaggccgc catcttggtc
     1801 gacgatttcg tcgacagcac cgtcgtgcgc gggaaaacgg cgccggagcg ggtggcgacc
     1861 tgggtggtcg tcacggactt ggtccccgca ggaagcgcgg agtagccgga caactggccc
     1921 tgaccggcat acagaaccgt gtcgtcggcc ttgatcgtca cgcggccgag gatgtctggc
     1981 tcgacgaggt cggcggagcc caactgggcg ttggtgctca gcggcggcgc ctcgacgttg
     2041 aaaacgccgt cgccgacgca cgccggacag atcttgaacg gcgcgctggc cggcggggcg
     2101 gcataccgct gatgcagggg gccgacaccc cagacgacgc tcttgatggt gcccggcttg
     2161 ggggaggcgg cgggcggtga ggcgaaactg cccttggtgg tgaatgtcgg gtcgaagctg
     2221 atgtattggt ccaggcgagc gctgtggtcg agcccgggcc cagcggtcgc gtaggtggtg
     2281 aggcggtcgc cgggcagtgg cgccccgagc gaccagccgc cgccgtgacc cgtgaccgcc
     2341 cgcgcgaaac tcgccgacgc gagacttcgc actccggtga agcgatcctt gtaggtcatc
     2401 aggttcgagg cgtcgctgtg cttgacgatc gtgccgtcgg ggatgcgccc gtcgatggag
     2461 tagtgggtgg cgaagcggta tgcgggggtg accccgctcg gtgagacggc gaggtagtcc
     2521 ttggtcaacg actggatgcc gtgcaaggtc ccgacggtcg ggacgactcg cacggagaag
     2581 ctgtcgtcac cgagggcgct gagactgaag cccacgccgc cggtcggtcc ctggatctca
     2641 cgattgacgg actgtgccca gaacgggttg acgacgggct tgttgacgct cgtcttgagc
     2701 cgcatggtgg ccttgcgcag gtcgaggacg atttcgtccc cgtcgcccac ggtggtctcg
     2761 cggcccgcgc ccatcgcaac gaccggtttg ccggccacct cggtgaacgt gctccccagc
     2821 agctgatagg tgccggcggg aacgctgatc ttggcgatgc cgtgcgtggt gaaaaccatc
     2881 ccgttgccct tgcccggatc cacgacattg gtgaacccga ccaggtcatc cagcgggcgt
     2941 ccgtcgaggc cgatgagccg gatggtgacg gtgaccatgg gccagcgtgg cgtggccgcc
     3001 ggctgcgcgg gtggcgcgac cgtgcggacg tgggcgaaga gggtcttggc gtccgtcgtc
     3061 gccagcgcgc gaccgagcgc ggcgctgctg gcggcgtcga cataaccggt ggcggtcgtg
     3121 cccgtgcgag cggtgatcac gatgcccggg acggccgtgg gcgccgagga actggcgtag
     3181 gtgacgttga cggcggtgcg gccgctaccg cgcgcggcga gagtgccgac gtcgaagagg
     3241 gccttgtcga agacccggcc tgcaactcct gacatgcgcc gggggatgac ataggtcgtc
     3301 gtcccgatcg tcatgctgcg cgcccccgcc ctggccaggt cagccggcgc agtcacagtg
     3361 ggcgccgcgc ccggattgcg ttgcacgcca acgcgatccc cattcgggag gacggccact
     3421 cctgttgaga cgctggatgg cgccgttggc gtccaggtgc gggagggcgg cctggtgccg
     3481 gctgccgggg cggggctcga ggccgtcgcc gtgccggcac cgacgccggc ggccaggacc
     3541 gtgctgcccg ccatcgccgc gatgaggttc ttcttcatcc gtcacgcctt tcggtgttga
     3601 ggtttggcaa acggtgagtg tccgaagacc gctccggtgt ttcaggcggg tgggatctcg
     3661 agcggggagg acgacatggc gccccaccgg cgcgtgcttc gagacgtggc aacgtgatcc
     3721 atcccctgcg tggcgccgtc cgggctcgtg cagacggtgt ctgcgctcgt cggtgcctga
     3781 cccgatcctc acaccccaca taccgggtat gtcaaggctt cgttgcggcg aaatctctgc
     3841 ggaccgcatc gcgcccgtga cctgctcgcc agcggcggaa agtcgctccg agactcagcg
     3901 gccgaggacg tgccgggaga tgaccaggcg ctggatctgg ttggtgccct cgaagatctg
     3961 cgtgactttg gcctcgcgga agtagcgctc cacggggaaa tcctgggtgt agcccgcgcc
     4021 accgagcacc tgcaccgcgt ccgtggtcac cttcatcgcg gcatcggtgg ccacgagctt
     4081 ggcgatggcg gcctccttgc tgaaggggcg cccggcatcg cgcaggcgag cggcatgcac
     4141 gtaggtggcg cgggcggcgc cgatcgccgc agccatatcc gcgatgacga acgccaaccc
     4201 ctgattcgac gcgatcggcc ggccgaactg ctcgcgctcg acggcataca gtgccgccaa
     4261 gtcgagcgcg ccctgtgcca aacccgttgc gccagcagcg attccgaggc gcccggagtc
     4321 aagcccagct agcgcgatgg gcaccccctg gccctcctcg ccgatccgcc gcgaggcggg
     4381 cacgcgcaca tcctcgtaga tgacctcccg caccgtgtcg ccgtgcagtc ccatcttgcg
     4441 ctccggcgcg ccgaaggaga ggccaggtgt gtcggcggga acgatgaagc acgacacccc
     4501 acgccccccg tcgtcagaag tgcgcgcaaa ggtcgtatag aagtccgcgt gcccggcgtg
     4561 ggagatccac gccttcttgc cgttgatgac gtagtcgtcg ccgtcgcggc gggcgcgcgt
     4621 ggtcatcgag gcgatatcgc tgccggcctg ggtctccgaa agggcgtagg cacccagggt
     4681 ctcgccggag agcatgtcgg gcaggaggtc ggccttctgt tcatcagtgc cataggtgaa
     4741 aaccgggtag cacgtcagcg tatgaaccga gatgcccagc ccggctgcca tccacgcgct
     4801 cgaaagctct tccagcactt gcagatagac ctcgaagggc tgcccggccc caccgaactc
     4861 ctccggatag ggcagcgaca tcaacccggc ctggccgagc atccggaaga cgtcacgcgg
     4921 gaaggcgtcg ctcttctccg cggccgcctc ggaagcatcg gcccgcggcc gcagctcctt
     4981 ctcggtgatc tctcggacca gatcgatcag g
//
LOCUS       DAOPJG010000220         5001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_18224_length_5001_cov_6.196118, whole genome shotgun sequence.
ACCESSION   DAOPJG010000220 DAOPJG010000000
VERSION     DAOPJG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18224_length_5001_cov_6.196118"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(411..728)
                     /locus_tag="PKU75_15020"
     CDS             complement(411..728)
                     /locus_tag="PKU75_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82237.1"
                     /translation="MNKLGRIRPDGKAVVFSSLTSNLVTGDTNGQRDVFIRDLVANTT
                     KRISLTGTGGQVHDAYPGVDYSTDGKKIGWFRNECDTAACSSGTGYLMQWSAAGQVRR
                     RRG"
     gene            complement(864..2111)
                     /locus_tag="PKU75_15025"
     CDS             complement(864..2111)
                     /locus_tag="PKU75_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPF82238.1"
                     /translation="MTPGGPGPHPVLAQVVREDWGRLLGLLIRRHGRPDLAEDALAEA
                     LASAAEQWPRDGIPDNPQGWLTQVASRRLLDAIRREATAAGKAHLLAVPEEAPPRHTH
                     GPGGTEDDRLPLLFMATHPALAPEVRPALALRFVLGVPTADIAALFLVPTTTMAARLT
                     RAKQRLRAIGAGFRVPEEPDLGARVDDVARAIYLAFTAGYAPREGEAVVHVEATSEAV
                     RLAHLTRELLPNTVVLPALCALLTLQHARRDARTDEQGGLVRLADQDRSLWRRDEIDA
                     GLVLLQGLSPTKGYAEELRLQALVAAFHDSAATSAETDWAAIARTYARLERLTGSPIV
                     RLNRAVAVAEVSGPMAGLRLLQAAADQLPGHHRIHVVRAGLLRREGDTEGARAAYDQA
                     LKTCPDGPERRHLLSRLGELPPA"
     gene            complement(2143..2496)
                     /locus_tag="PKU75_15030"
     CDS             complement(2143..2496)
                     /locus_tag="PKU75_15030"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPF82239.1"
                     /translation="MKYLVLLIGDGEEQPWPSLTEAEQGALMQKFGEFDAECQAREGV
                     EILGGEALGGPSDATVMRTRGGEVTLTEGPYAEALEGLGGFYLVESPDLDTLVDCLRK
                     LPRYDMQIAPVLDMS"
     gene            complement(2673..3731)
                     /locus_tag="PKU75_15035"
     CDS             complement(2673..3731)
                     /locus_tag="PKU75_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF82240.1"
                     /translation="MTSFWALTRAQWFGFWRDKQNWFWLLAFPLMFLVLFGFLFRDAG
                     ASKSSLTQIGSVAIIDQLPAEAKAQFDNLFEVTKTDDAAAALEEVRKGDVDGAVEQKG
                     NEVILHYSQADQVKAATVRGTLGAFVDGANVAMSGQPPTLTLKAEAAEDDSLKPIQFI
                     APGLLGWAVAMGATFNAAMPFVTWRTNKLLRRLRLAPMRTESLVSSRLLVSIAVAMIQ
                     MGVFLAVGVGLFDLKLTGSWWMAIPLLLCATIAFMAIGLIAGAISKTAEAASGIANVI
                     ILPMAFLSGSFIPLDQAPSWLQTIAKFLPLGQLNEGLLDTLVRGEGPGAALMPMVYLL
                     GFAVVFGLIAARLFRWED"
     gene            complement(3728..4630)
                     /locus_tag="PKU75_15040"
     CDS             complement(3728..4630)
                     /locus_tag="PKU75_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF82241.1"
                     /translation="MTNVIETRGLVKRYGDLTAVDHVDITVPQGTFFGLLGPNGAGKT
                     TTLEMIEGIREPDEGDVRLFGEKVWPRNRAVLPRIGVQLQASAFFERLSVEEQLRCFA
                     DLYAVDHKQIDRMIEMVGLQEKRKTRTEDLSGGQKQRLSIACSLIHDPEVVFLDEPTA
                     ALDPQARRNLWDVLRAINDEGRTVVLTTHYMDEAEILCDQIAIIDRGQILTTDTPRNL
                     ITGLGSAYRILVSEGVLSDQEAAALPGVDSASLSDGTLAIITHTPSPVLAALAERDAL
                     QGLQVTGATLEDVFLTLTGREYRA"
     gene            complement(4694..>5001)
                     /locus_tag="PKU75_15045"
     CDS             complement(4694..>5001)
                     /locus_tag="PKU75_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82242.1"
                     /translation="AEVWKAARSRPKERLTVSQINRRTLIQASGAAGLTMLTPGLARA
                     ATLPGRHDYGDIRDIEHTDLASYCLTVRASPIPVCSTSQAPRTAGGIPSRHSRDRNR"
BASE COUNT          781 a         1638 c         1784 g          798 t
ORIGIN      
        1 ccgtgcaggc ggcggactgg cccgtcggga ggctgacggt ggtcgcggtg gtgccggccg
       61 tgtcgcacag gcacacctgc tgcgagatcg gggtgttcca gaattcggcg tcgaacgcga
      121 tcttgacgcc gtccggggtg atggcgaccc cgtagacagc catctggctg gcaggcaacg
      181 tctgccacgt cttgcccttg gtgaactttg aggttggtgg attcgcccag cgcgcccacg
      241 gtgccggacg cggcgatgtc gaaggcgcat gtctcgcagt cgaagccata gacttcactg
      301 gtcggggtgg cgatcgagat gtagccgtca tgctcgcccc acgagctgac ctcgacagcg
      361 ttcaggccag cacggatggg cccgatggaa agggagactc cgaccacgac ttagccgcga
      421 cgacgacgaa cttgcccggc cgccgaccat tgcatgaggt atcccgtccc ggagctgcac
      481 gctgcggtgt cgcattcgtt gcggaaccag ccgatcttct tgccgtcggt ggagtagtcg
      541 acaccggggt aggcgtcgtg gacctggcca ccggttccgg tcagcgagat gcgtttggtg
      601 gtgttggcga ccaggtcgcg aatgaagacg tcgcgctggc cgttggtgtc accggtgacc
      661 aggttggacg tgagtgaact gaagacgacc gccttgccgt cgggccggat gcggcccagc
      721 ttgttcatgc cctttccctt cccgagaggt gccccacgaa atgtgcgggc agaccgcggg
      781 acgcgcgtgc tgccgcacga cacatcgtgg cgggcaacga ccgcgcaggt gccggaatga
      841 cgagattcgt gccggcggaa gacctatgcc ggcggcagct cgccgagccg ggagagcagg
      901 tggcggcgct cggggccgtc ggggcaggtc ttcagggctt ggtcgtatgc cgcccgcgca
      961 ccctcggtgt cgccctcgcg gcgcagcagc ccggcgcgca ccacatggat gcggtggtga
     1021 ccgggcagtt ggtcggcggc ggcttggagg aggcgcaggc cggccatcgg gccgctcacc
     1081 tcggcgacgg cgaccgcccg gttgaggcgc acgatcggcg agccggtgag gcgctccaac
     1141 cgggcgtagg tgcgagcgat ggcggcccag tcggtctcgg cggaggtggc ggcgctgtcg
     1201 tggaaggcgg cgaccagcgc ttgtaggcgc aactcctccg catacccctt ggttggcgaa
     1261 agcccctgca ggagaaccaa acccgcgtcg atctcgtcgc gacgccagag cgaccggtcc
     1321 tggtccgcaa gccgcacgag cccgccctgc tcgtcggtgc gcgcgtcacg gcgggcgtgc
     1381 tgcagcgtga ggagtgcaca aagggctggc agcacaacag tattcggcag cagttcgcgg
     1441 gtgaggtggg ccaggcggac cgcctcgctc gtcgcctcga cgtggacgac ggcctccccc
     1501 tcgcgcgggg cgtagccggc cgtgaaggcc aggtagatcg cccgcgcgac atcgtcgaca
     1561 cgggcgccga ggtccggctc ctcgggcacg cggaagccgg cgccgatcgc gcggagccgc
     1621 tgctttgcgc gggtgagccg ggccgccatg gtggtggtgg ggacgagaaa gagcgcggcg
     1681 atgtcggcgg tcgggactcc gaggacgaac cgcagcgcga gcgccgggcg cacctccggc
     1741 gccagcgcgg ggtgggtggc catgaagagg agcggcaatc ggtcgtcctc ggttccgccc
     1801 gggccgtggg tatgccgcgg cggcgcctcc tccggcaccg cgagcaggtg cgccttcccc
     1861 gccgcggtcg cctcccgccg gatggcgtcg aggagacgtc gggacgccac ctgcgtcagc
     1921 cacccctgcg ggttgtccgg tatgccgtcg cgcggccact gctccgcggc gctcgccagg
     1981 gcctcggcca gcgcgtcctc ggcgagatcg gggcggccgt gtcggcggat cagcagcccg
     2041 agcagccgcc cccagtcctc gcgcacgacc tgcgcgagga cggggtgcgg gccgggcccg
     2101 cccggcgtca cggcataccc tcagctgttc gacggggccg actcaggaca tgtcgagcac
     2161 gggcgcgatc tgcatgtcat agcgcggcag cttcctcagg cagtcgacga gggtgtcgag
     2221 atcgggggac tcgacgaggt agaagccgcc gaggccctcg agcgcctcgg catacggccc
     2281 ctcggtgagc gtgacctcgc cgccgcgggt gcgcatgacc gttgcgtcgg acggcccgcc
     2341 gagggcctcc ccgccgagga tctcgacgcc ctcgcgcgcc tgacattcgg catcgaactc
     2401 gccgaacttc tgcatcagcg cgccctgctc cgcctcggtg aggctcggcc acggctgctc
     2461 ctcgccgtcg ccgatcaaca agaccaagta cttcatggtg tgctccttcg caattcgtct
     2521 cgacggcggg cgccgtcacc atggtgtcgc gcgagggcga gggaaatcga cacaactccc
     2581 atgtcgaaag tgcaccccca cgtcgaaagt gcacgacacg ccgtaatgcg acacgcatta
     2641 cggcgtgtcg tgcgctttcg acgtgggcgg ggtcaatcct cccagcggaa gaggcgggcg
     2701 gcgatgaggc cgaagacgac ggcgaagccg aggaggtaca ccatcggcat cagcgcagcg
     2761 ccgggacctt cgccgcggac cagggtgtcg agcagaccct cgttgagctg gccgagcggg
     2821 agaaacttcg cgatcgtctg cagccagctc ggcgcctggt cgagggggat gaaggaaccc
     2881 gacaggaagg ccatcggcaa gatgatgacg ttggcgatgc ccgaggccgc ctcggcggtc
     2941 ttggagatgg cgccggcgat caggccgatg gccatgaagg cgatcgtcgc gcacagcagc
     3001 agcggtatcg ccatccacca cgagccggtc agcttcaggt cgaacagccc gaccccgaca
     3061 gccaggaaga cgcccatctg gatcatggcg acggcgatcg agaccagcag ccgcgaggag
     3121 accagcgact cggtgcgcat cggcgccagc cgcagccgcc gcagcagctt gttggtccgc
     3181 caggtcacga agggcatcgc ggcgttgaag gtcgcgccca tcgccacggc ccagccgagc
     3241 aggccgggcg cgatgaactg gatgggtttg agtgagtcgt cctccgccgc ttcggctttg
     3301 agggttaagg tcgggggctg gccggacatc gcgacattgg cgccgtcgac gaaagcgccg
     3361 agcgtcccgc gcacggtcgc cgccttgacc tggtcggcct ggctgtaatg caggatgacc
     3421 tcgttgccct tctgctccac cgctccgtcg acgtcgccct tgcgcacctc ctccagcgcc
     3481 gctgccgcgt cgtcggtctt ggtgacctcg aagaggttgt cgaactgcgc cttggcctcc
     3541 gccggcaact ggtcgatgat cgcgaccgag ccgatctgcg tcaggctgct cttgctcgct
     3601 ccggcgtccc ggaagaggaa cccgaagagc acgaggaaca tcagcgggaa ggcgagcagc
     3661 cagaaccagt tctgcttgtc gcgccagaac ccgaaccatt gcgcccgcgt cagcgcccag
     3721 aagctggtca tgcgcggtac tcccgtccgg tcagggtcaa gaagacgtcc tcgagtgtcg
     3781 ctccggtgac ctgaagtcct tgcagcgcat cgcgttcggc gagcgctgcg agcacggggg
     3841 agggggtatg ggtaatgatg gccagagtcc cgtcgctcag gcttgcgctg tcgacgcccg
     3901 gcaacgctgc ggcctcctgg tcgctcagga cgccttcgct gacgaggatc cggtatgccg
     3961 atcccagccc ggtgatcaga ttgcgggggg tgtcggtggt caggatctgg ccgcggtcga
     4021 tgatcgcgat ctggtcgcac aggatctcgg cctcgtccat gtagtgggtc gtcaggacga
     4081 ccgtgcggcc ctcgtcgttg atggcgcgca gcacgtccca taggttgcgg cgagcctgcg
     4141 ggtccaacgc agccgtcggc tcgtcgagga agacgacctc cgggtcgtgg atgagtgagc
     4201 aggcgatcga cagccgctgc ttctgcccgc cggagagatc ttcggtccgg gtcttgcgct
     4261 tctcctgcag cccgaccatc tcgatcatgc ggtcgatctg cttgtggtcg acggcataca
     4321 ggtcggcgaa acagcgcaac tgctcctcca cggacagccg ttcgaagaag gccgaggcct
     4381 gcaactgcac gccgatgcgg ggcagcacgg cgcgattacg cggccaaacc ttctcgccga
     4441 agagccgcac gtcgccctcg tcgggttccc ggatgccctc gatcatttcg agggtggtgg
     4501 tcttgccggc cccgtttggt ccgagcaagc cgaagaaggt gccttgcggc accgtgatgt
     4561 cgacatggtc caccgcggtg aggtccccgt acctcttgac cagcccgcgc gtctcgatga
     4621 cgttggtcat gcgccgatcc aaacacgaga gcgaccaccg tcgccaccgg ttgaggatct
     4681 tctttgccgg gaattagcga ttccggtcgc gcgaatgcct gctgggaatg ccaccagccg
     4741 tgcggggtgc ctgcgaggta ctgcataccg ggatcgggga cgcccggacg gtaaggcagt
     4801 acgacgccag atccgtatgt tcgatgtcgc ggatgtcacc gtagtcgtgg cgcccaggca
     4861 gggtcgctgc tcgggcgagg cccggtgtca gcatggtgag gccggctgct ccgctggcct
     4921 ggatgagggt gcggcggttg atctgggaca cggtcaatcg ctccttcgga cgagatctgg
     4981 ccgccttcca cacttctgcg c
//
LOCUS       DAOPJG010000221         4936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_18541_length_4936_cov_7.639828, whole genome shotgun sequence.
ACCESSION   DAOPJG010000221 DAOPJG010000000
VERSION     DAOPJG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18541_length_4936_cov_7.639828"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PKU75_15050"
     CDS             <1..728
                     /locus_tag="PKU75_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPF82243.1"
                     /translation="SLKTPIAQTLHATGYAMGVLRDDAVGTGDPERDTAVIAYFGDGA
                     SAQGDVGEAFVFAGVNNAPVVFFCQNNQWAISEPNERQMRIPIYLRPRGYGFPGIRVD
                     GNDVLATYAVTTACLDAARDGQGPSLIEAFTYRMGAHTTSDDPTKYRVSAEVEVWKLR
                     DPIARLRNLLARTYGVGQEFFDGVDAEAEEVGVDLRTRLLALPDPEPESMFEHVYAAP
                     HPVMDRERAALSDYLASFEGAEA"
     gene            728..1699
                     /locus_tag="PKU75_15055"
     CDS             728..1699
                     /locus_tag="PKU75_15055"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HPF82244.1"
                     /translation="MAQMTMAKALNAGLRAAMDKDPKVVLMGEDIGKLGGVFRITEGL
                     QKDFGEDRVIDTPLAEAGIVATAIGLTLRGYRPVVEIQFDGFVYPAFDHIVSQVAKLH
                     ARSLGHVKLPMVIRIPVGGGIGAVEHHSESNEAYFAHTAGLRVISCSNPSDAYWMIQE
                     AIEFPDPIIFYEPKRRYWDKGEIAEAPERDLFSANVIRPGTDVTILAYGPMVRSCLQA
                     AQAAEEEGYSLEVIDLRSLSPLDTDAVFGSVRKTGRCVVVHEASTFLGMGAELAAQIQ
                     QECFYSLEAPVLRVGGYNVPYPPSRLEEDFLPDLDRVLDAVDRVMAH"
     assembly_gap    1826..1925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1926..3565
                     /locus_tag="PKU75_15060"
     CDS             <1926..3565
                     /locus_tag="PKU75_15060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPF82245.1"
                     /translation="RDFKLPDPGEGLTEADIVTWRVAVGDTVKVHDIVVEVETAKSLV
                     ELPIPYAGTVAALLVAEGDTVEVGTPIITIDDGSSAPAAANDGSATDRPAPASAPSPA
                     VAGGQAGSGEAGGPTALESNSPTGSVDESDADKKPERTAVLVGYGVKQTEAKRRPRRK
                     QNLLQTIAHYPDEEPAVRETTAHADASSWVGELVAAHSTHVLRDRRPLTELTDHSPEA
                     LARARVLAKPPVRKLAKDLGVDLVGLRGSGDDGTITREDVLAASRGGAPTATGGPTGS
                     GAPAAMTAAPAGGGAEAGGPHTTGAAGPYSRERGEERTPIKGVRKVTAAAMVSSAFTA
                     PHVSEWVEVDVTATMELVERLKRDKEFRDVKVTPLLVLAKAMCLAVRRNPGVNATWDE
                     AAQEIVVKHYVNLGIAAATPRGLLVPNIKDADLLSMRDLGAAINALTETAREGRTQPA
                     EMAGGTITITNVGVFGVDAGTPIINPGESAILAFGSITRKPWVVGRGDDERIEPRWVT
                     TLTLSFDHRLVDGELGSRYLADVAAILADPAKGLVWG"
     gene            complement(3626..>4936)
                     /locus_tag="PKU75_15065"
     CDS             complement(3626..>4936)
                     /locus_tag="PKU75_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPF82246.1"
                     /translation="MAGDVLLPGDTAYAGAKQLFAPRYDVLRPLAVVRPATTADVATA
                     VKFARAHDLRVTARAGGHSYTGRSSGNALMVLDLRKLTGIQLAGAGATAVLGAGTTLY
                     NVHAKLVTSGRTITTGTCPTVGTTGLALGGGLGPDDHRHGLTADSVTGATMVLMDGRT
                     VTVSNSQPDLLWALRGGGGQLGIVTQLRMATHAASSRGYLDLAFPGSAAQAVIAAWAA
                     YQPAASAGTWVRAQITRSAAGGARCQLLGVTDAGMQNRVAQDLIDAVGVTPLSRTVKQ
                     LTHLQLVTTLGNGTTTPRRDNDHGSDVLTDVSSTTAAGIVAAMGSSPGTGGGCVVQLS
                     PLGGGIAARTPTGTPFPYRRHIGAVQWVTGLPTGATAADFAAARAWIDAAHAQVSAAS
                     VGGYVNYVEPGQPAARYFGANLTRLRSLKTTYDPEGRLLAAVDL"
BASE COUNT          738 a         1703 c         1678 g          717 t
ORIGIN      
        1 cgtccctcaa gacacccatc gcccagacgc tgcatgccac cggctacgcg atgggtgtct
       61 tgcgggacga cgccgtcggc accggcgacc ccgagcgcga caccgccgtc atcgcctact
      121 tcggcgacgg tgcctccgcg cagggcgatg tgggggaggc gttcgtcttc gcgggcgtca
      181 ataatgcgcc cgtcgtcttc ttttgccaga acaaccagtg ggccatctcc gaaccgaacg
      241 agcgccagat gcgcatcccg atctacttgc gcccgcgcgg atacggattc cccggcatac
      301 gggtggacgg caacgacgtg ctggccacgt atgccgtgac caccgcctgt ctcgacgccg
      361 cccgcgacgg ccagggcccc tcgctcatcg aggccttcac ctatcggatg ggtgcgcaca
      421 cgacgtccga cgacccgacg aaataccgcg tgtcggccga ggtcgaggtc tggaagctgc
      481 gcgacccgat cgcccgcctg cgcaacctgt tggcgcgcac gtatggcgtg gggcaggagt
      541 tcttcgacgg cgtcgacgcc gaggccgagg aggtcggcgt cgacctgcgc acccggctgc
      601 tcgcgctgcc cgacccggag ccggagtcga tgttcgagca tgtgtatgcc gcgccgcacc
      661 ccgtcatgga tcgcgagcgt gccgccttga gcgactacct cgcgagtttc gaaggggctg
      721 aggcgtgatg gcgcagatga ccatggcgaa agccctcaac gccggtctgc gcgcggcgat
      781 ggacaaggac cccaaggtcg tgctcatggg cgaggacatc ggcaagctcg gcggcgtctt
      841 ccggatcacc gagggcctgc agaaggactt cggcgaggac cgggtcatcg acaccccgct
      901 cgccgaggcc ggcatcgtcg ccacggccat cggtctgacg ttgcgcggct accggccggt
      961 ggtggagatc cagttcgacg gcttcgtcta ccccgccttc gatcacattg tctcccaggt
     1021 cgccaagctg cacgcccgct cgctcgggca cgtgaagttg ccgatggtga tccgcatccc
     1081 tgtcggcggc ggcatcgggg cggtggagca ccactcggag tcgaatgagg cctacttcgc
     1141 gcacaccgcc ggactgcggg tgatctcctg ttcgaacccg tcggatgcct actggatgat
     1201 tcaggaggcg atcgagttcc ccgacccgat catcttctac gaacccaagc ggcgctactg
     1261 ggacaagggc gagatcgccg aggccccgga gcgggatctc ttctccgcca atgtgatccg
     1321 gcccggaacg gacgtgacga tcctcgccta cggtccgatg gtgcgctcgt gcctgcaggc
     1381 cgcgcaggcg gccgaggagg aggggtattc gctcgaggtc atcgacttgc gctcactgtc
     1441 cccgctggac accgatgcgg tcttcggctc ggtgcgcaag accggccgct gcgtcgtggt
     1501 ccacgaggcc tcgaccttcc tcggcatggg tgccgagctg gccgcgcaga tccagcagga
     1561 gtgcttctat tcgctggagg cgccggtgct gcgcgtcggc ggctacaacg tgccctatcc
     1621 gccgagccgg ctcgaagagg acttcctgcc cgacctcgac cgcgtgctcg acgccgtcga
     1681 ccgcgtcatg gcgcactaac cccgacgaaa gttgctcgct gcgctcgcct tacttcgcgg
     1741 gcccgaggaa taggagactt ggaaacacaa tgcgagactt caaacttccc gaccccggtg
     1801 aaggtctgac cgaggcggac atcgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnntgcga gacttcaaac ttcccgaccc cggtgaaggt ctgaccgagg cggacatcgt
     1981 cacctggcgc gtcgccgtcg gggacacggt caaggtccac gacatcgtcg tcgaggtcga
     2041 gacggcaaaa tcgttggtgg agttgccgat cccgtatgcc ggcacggtcg ccgctctgtt
     2101 ggtggccgag ggcgacaccg tcgaggtggg caccccgatc atcacgatcg acgacggctc
     2161 cagcgctccg gcggctgcga acgacgggtc cgcaaccgac cggccggcgc ccgcatccgc
     2221 tccgtcgccg gctgtcgccg gcgggcaggc cggctcgggt gaggcgggcg gacctaccgc
     2281 gctggagtcg aattcgccga ctggctcggt ggacgagtcc gatgcggaca agaaacccga
     2341 gcgcaccgcc gtgctcgtcg gctacggcgt caagcagacc gaggccaagc gccgtccccg
     2401 gcgcaagcag aatttgttgc agacgatcgc gcactacccc gacgaagagc ccgcggtccg
     2461 cgagaccacc gcccacgcgg acgcgagcag ctgggtcggc gaactcgtcg ctgcccattc
     2521 cacccacgtg ctgcgcgacc gccggccatt gaccgagctg accgatcact cgccggaggc
     2581 gctcgcgcga gcccgcgtgc tggccaagcc gccggtccgc aaactcgcca aggacctcgg
     2641 tgtcgacctc gtcggcctgc gcggcagcgg tgacgacggc accatcaccc gcgaggacgt
     2701 gctcgccgcc agccgcgggg gcgcacccac cgcgacgggg ggacccacgg gatccggagc
     2761 cccggcggcg atgaccgcgg cccccgcggg cgggggtgcc gaggcgggcg gtccgcatac
     2821 caccggcgcc gcaggcccat actcccgcga gcgcggggag gagcgcaccc cgatcaaggg
     2881 ggtgcgcaag gtcaccgcgg cggcgatggt ctcctcggcg ttcaccgcgc cccacgtcag
     2941 cgagtgggtc gaggtcgacg tcaccgcgac catggaactg gtcgaaaggc tcaagcggga
     3001 caaggagttc cgtgacgtca aggtcacgcc gctgctcgtc ctcgccaagg cgatgtgctt
     3061 ggcggtgcgg cgcaatccgg gcgtcaatgc gacctgggac gaggcggccc aggagatcgt
     3121 cgtcaagcac tacgtcaacc tcggcatcgc ggccgccacc ccgcgcgggc tgctcgtgcc
     3181 gaacatcaag gacgccgacc tgctctcgat gcgcgacctc ggcgcggcga tcaacgcgct
     3241 gacggagacg gcgcgcgagg gcaggacgca gccggcggag atggcaggcg ggacgatcac
     3301 gatcacgaat gtcggtgtct tcggcgtcga tgccggcacc ccgatcatca acccgggcga
     3361 gtcggcgatc ctggcgttcg ggtcgatcac ccgcaagccg tgggtggtcg gccgcggcga
     3421 cgacgagcgg atcgaaccgc gttgggtcac gacgctgacg ctgtccttcg accaccgcct
     3481 cgtcgacggg gaactcggct cccgctacct cgccgacgtg gccgccatcc tcgccgatcc
     3541 cgccaagggc ctcgtctggg gctgaccggc cgagctcatc gcatgggcgg tcttggtcgc
     3601 accggattcc tgggtagtcg tgcgatcaga ggtcgacggc ggcaaggagc cggccttcgg
     3661 ggtcgtaggt ggtcttgagc gagcgcaacc gggtcagatt ggcgccgaag tagcgggcgg
     3721 cgggttgtcc gggctcgacg tagttcacat agccgccgac gctcgcggcg gagacctgcg
     3781 cgtgtgccgc gtcgatccac gcgcgggccg ccgcgaagtc ggcggccgtc gcgccggttg
     3841 gcagcccggt cacccattgc acggccccga tatgccgccg ataggggaag ggcgtgcccg
     3901 tgggcgtgcg cgccgcgatc ccgccaccga gggggctgag ctgcaccacg cagccgccgc
     3961 ccgtcccggg actcgacccc atcgccgcga cgatcccggc agcggtcgtc gacgacacgt
     4021 cggtgaggac gtcgctgccg tggtcgttgt cgcggcgcgg cgtggttgtg ccattgccca
     4081 gggtcgtgac gagttgcagg tgcgtcagct gcttgaccgt ccgggacagc ggagtgaccc
     4141 cgaccgcgtc gatgaggtct tgcgcgacgc ggttttgcat gccggcgtcg gtgaccccga
     4201 gcagctggca acgggcgccg cccgccgcgc tgcgagtgat ttgggcgcgc acccaggtgc
     4261 cggcgctcgc cgcgggctgg tatgccgccc acgccgcgat gaccgcctgc gccgctgatc
     4321 cggggaaggc caggtcgaga tatccgcgcg agctcgcggc atgggtcgcc atccgcagct
     4381 gggtgacgat gcccaactgg ccacccccgc cgcgcagggc ccacagcaga tccggctggc
     4441 tgttcgagac ggtgaccgtc cggccgtcca tgagcaccat cgtggctccg gtgacgctgt
     4501 cggccgtcag gccgtggcga tggtcgtccg gcccgagccc gccgcccagt gccagccccg
     4561 tcgtcccgac cgtgggacag gtccccgtcg tgatcgtgcg cccggacgtg accagcttcg
     4621 cgtgcacgtt gtacagcgtc gtccccgcgc ccagcaccgc ggtggcgccc gcgccggcca
     4681 gctggatccc ggtcagcttg cgcaggtcga gcaccatgag cgcattcccg gaggaccggc
     4741 cggtatacga gtggccgccc gcccgcgcgg tcacgcgcag atcgtgtgct cgcgcgaact
     4801 tcaccgccgt cgcgacgtcg gccgtcgtcg ccggccggac gacggcgagg ggtcgcagca
     4861 cgtcataccg tggagcgaaa agctgcttcg ccccggcgta tgccgtgtcc cccggcaaca
     4921 gcacatctcc cgccat
//
LOCUS       DAOPJG010000222         4874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_18874_length_4874_cov_8.584354, whole genome shotgun sequence.
ACCESSION   DAOPJG010000222 DAOPJG010000000
VERSION     DAOPJG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18874_length_4874_cov_8.584354"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1292)
                     /locus_tag="PKU75_15070"
     CDS             complement(240..1292)
                     /locus_tag="PKU75_15070"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPF82247.1"
                     /translation="MSSAPLPWARVTDHLGTMADDGRFNGILLVMQADATVVEFCRGW
                     ADRAAGVQIHPGTRFAVASMSKMFTAAATLLAVGRGELAVTDRIVDLLPEARRPAILS
                     SEITVHHLLTHTSGIGDYFEEDESLPHYTGDDYGATWAGRNPGRVERPDDYLPLYADR
                     APAAAPGEWHYSNAGYVLLGAVLEEMTGRAYVDVVTEDVLIPAGMADTGYFRSDEPRP
                     DVAQAYIDRSGAAWRTNVFSVPVIGGGDGGALATARDIDRFLRVIADGSLLGPELSAL
                     MRTPHVPAYGDRSQGYGPIVAPGGIFQHGGGDPGVAVFARHHGDEDLSVVVLLNVDGV
                     FDDVWDVVSSGLPTHP"
     gene            complement(1289..2323)
                     /locus_tag="PKU75_15075"
     CDS             complement(1289..2323)
                     /locus_tag="PKU75_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPF82248.1"
                     /translation="MLQLPAARTPDPREAPSLRWGILGTGWIADKFVTALQRNTTQQV
                     HAVGSRSQEGADRFASAVGASAAYGSYEALVADPDVDIIYVATPHNHHHANAVLAIDA
                     GKHVLIEKPIAISEAEAQDIADRAAAAGVFCMEALWTLFLPKYDVLRQLLDDMVVDTV
                     LADMGEYFTPDHRIFRADLAGGPLYDMATYPLSFAMWALGDPERIAAAGTPAPNGING
                     QLAMQLVGADGGVAALHCSIIGETPTTGVIASADRTITIDGPFYQPGGFTVTTRGGES
                     ARYDEEPVAHEGGLHFQAAEVARLITAGARESDVRPLAHSIRLLRVMDAIREQVGIPL
                     PAGVRRVRCR"
     gene            complement(2397..3464)
                     /locus_tag="PKU75_15080"
     CDS             complement(2397..3464)
                     /locus_tag="PKU75_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF82249.1"
                     /translation="MAHGHRIADIATQAGLSVATVDRVLNERPHVSAQARRQVEAAIA
                     ELDRQHTQLRLDGISLVIDVVMQAPKRFSDAVRASLESQLASLRPALVRARFSLRESG
                     TAEELASQLDRIGRGGHVSDGVLLKAPDDPVIAAAIDRLRERGIPVVTLVTDVQHCRR
                     IAYVGLDNAAAGATAAFLVAEWTTGAAGAVLVTMSRSTMIGEQERLTAFTATLARLTP
                     GRDTLTIADADGLDQGMSDLVRAATRKRRDIAAVYSIGGGNRAIATQLRRSRIHPRIH
                     LGHDLDADNDELLRTGALTAVLHHDLRADVRAGCEQILRFHRILPGVPTSVRATPQIV
                     TAYNLPPRLGPAAQPDLGSQT"
     gene            3616..4815
                     /locus_tag="PKU75_15085"
     CDS             3616..4815
                     /locus_tag="PKU75_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPF82250.1"
                     /translation="MSALAQAATRRTPAFPVGTLHAGDCRLADLLEILQDTTDLADYP
                     HASGVEQGVLLYAAADVRAALADGAGEELEAELARAWSQGPGIIVFTGAFESEIIDRV
                     NAAYDRIIKQEKESGGPKGDHFGEPGANDRVWNALEKLAVADPEAFVDYYSNDLVALG
                     ARAWLGPAYQITVQPNVVRPGGKGQTVHRDYHLGFMDSEQAARFPAHVHALSSVLTLQ
                     GAVAHVDMPVESGPTLYLPHSQKYTHGYLAYWIPEFQDYFVANHIQLPLTKGDLVWFN
                     PALMHAAGTNVSADIQRMANLLQVSSAFGRAMESVDRERVVNAVYPALLAALDGGMPR
                     PLVDNAVAASAEGYAFPSNLDRDQPVGGMAPPTQADLVHAALDTRETPDSLRDRLAHQ
                     AWTKLSH"
BASE COUNT          745 a         1687 c         1723 g          719 t
ORIGIN      
        1 agggtcagcg ctgccccgcg gcgggtgagg ccggcgccga aggcccgccc ggggccgccc
       61 acggcgggcg gcgaagtcgg cgacgcactt ctccccgaaa gcctttcgag gcttcggggc
      121 gaacgggtgg tcggctgcgg gcggaggtcg agcggcatac cgtcgaccgt aggaacccgc
      181 gcgccggtga tcgagccgcc cgcatcatcc gttcggatga gttggtcacg gatgattggt
      241 cacggatgag tgggcaagcc cgacgagacc acatcccaca cgtcgtcgaa gacgccgtct
      301 acgttcagca acacgacgac ggagaggtcc tcgtcgccgt gatggcgggc gaacaccgcc
      361 actcccggat cgccgccccc gtgctggaag atgcctcccg gcgcgacgat cgggccgtac
      421 ccctgcgagc ggtcgccgta tgccggcacg tgcggtgtcc gcatcagcgc gctgagctct
      481 ggcccgagga gcgagccgtc cgcgatgacg cgcaggaagc ggtcgatatc gcgggcggtg
      541 gccagggccc cgccgtcgcc gccgccgatc accgggacgg agaagacgtt ggtgcgccat
      601 gccgcgccgc tacggtcgat ataggcctga gcgacgtcgg gccggggttc gtcggaacgg
      661 aagtagccgg tgtcggccat gccggcgggg atgagtacgt cctcggtgac gacatcgacg
      721 tatgcccggc cggtcatctc ctcgagcacc gcaccgagca gcacgtagcc ggcgttgctg
      781 taatgccact ctccgggtgc ggcggcgggc gcgcggtcgg catacaaggg caggtagtcg
      841 tccgggcgct cgacccgtcc cggattccgg ccggcccacg tcgcgccata gtcgtcgccg
      901 gtgtagtggg ggagggattc gtcctcttcg aagtagtcgc cgatccccga ggtgtgggtg
      961 agcaggtggt gcacggtgat ctcggacgag aggatcgcgg ggcggcgagc ctcgggcagc
     1021 agatcgacga tgcggtcggt gacggccagc tccccgcggc cgaccgccag cagcgtcgcc
     1081 gcggcggtga acatcttgga catcgacgcc accgcgaagc gggtgccagg gtgaatctgc
     1141 actcccgcag cgcgatccgc ccagccgcga cagaactcca ccacggtcgc gtcggcctgc
     1201 atcacgagca agatgccgtt gaaccggccg tcgtcggcca tcgtgccgag gtggtcggtc
     1261 acccgggccc acggcaacgg tgcgctgctc atcggcacct cactcgcctc actccggcag
     1321 gcagcggtat gccgacctgc tcgcggatgg cgtccatcac ccgcagcagc cggatcgagt
     1381 gggcgagcgg gcggacgtcg gattcgcgcg ctcctgcggt gatgaggcgg gccacctcgg
     1441 cggcctggaa gtgcagcccg ccttcgtggg cgaccggctc ctcgtcatac cgggcgctct
     1501 cgccgccgcg cgtggtgacg gtgaacccgc ccggttggta gaagggtccg tcgatcgtga
     1561 tcgtccggtc ggccgacgcg atgacacccg tcgtcggcgt ctccccgatg atcgagcagt
     1621 gcagcgcggc gacgccgccg tcggcgccga ccagttgcat cgccagctgg ccgttgatgc
     1681 cgttcggcgc cggggtgccg gccgcggcga tgcgctccgg atcgccgagc gcccacatcg
     1741 cgaaggacaa agggtaggtc gccatgtcat agagcgggcc gccggcgagg tcggcgcgga
     1801 agatgcggtg gtccggtgtg aagtactcgc ccatgtcggc gaggacggtg tccaccacca
     1861 tgtcgtcgag cagctggcgc agcacgtcgt acttcggcag gaagagcgtc cacagcgcct
     1921 ccatacagaa aactcccgct gccgcagcgc gatccgcgat gtcctgggcc tcggcctcgc
     1981 tgatggcgat gggcttctcg atgaggacgt gcttgccggc gtcgatggcg agcaccgcgt
     2041 tggcgtggtg gtggttgtgg ggcgtggcga catagatgat gtcgacgtcc gggtcggcga
     2101 cgagggcctc gtaagagccg tatgccgcgc tcgcccccac cgcgctcgcg aaccggtccg
     2161 cgccctcctg actacgcgac ccgaccgcgt ggacctgctg agtcgtgttg cgctgcaacg
     2221 ccgtcacgaa cttgtcggcg atccagccag tcccgaggat gccccagcgc agggacggtg
     2281 cctcgcgggg atcgggggtc cgggcggcgg gaagctgaag catggggggt ccttccgagc
     2341 gcggtctttg tcagcacatt agagcaagag gggctcagcg ggctaggtct gactccctat
     2401 gtctggctgc ctaggtctgg ctgcgcggcc ggtccgagcc gcggggggag gttgtatgcc
     2461 gtgacgatct gcggcgtggc ccgcactgag gtcgggacgc cgggcagtat gcggtggaac
     2521 cggaggatct gctcgcatcc cgcgcgcacg tcggcgcgca ggtcgtgatg gaggacggcg
     2581 gtgagcgcgc cggtgcgcag cagctcgtcg ttgtcggcgt cgaggtcgtg accgagatgg
     2641 atgcgggggt ggatccggct gcgccgcaac tgggttgcga tggcgcggtt gccgccgccg
     2701 atggagtaga cggcggcgat gtcgcgccgc ttcctcgtcg cggcccgcac cagatcgctc
     2761 atgccctgat ccagcccgtc ggcgtcggcg atcgtgagag tgtcgcggcc gggggtgagc
     2821 cgggcgaggg tcgcggtgaa ggcggtcagg cgttcctgct cgccgatcat cgtcgaccgg
     2881 ctcatcgtca cgaggacggc acccgccgcg ccggtcgtcc actccgccac gagaaaggcc
     2941 gcggtcgctc ccgcggcggc gttgtcgagc ccgacatagg cgatgcgccg gcagtgctgg
     3001 acgtcggtga cgagcgtgac cacgggtatg ccgcgctcgc gcagccggtc gatcgcagca
     3061 gcgatgaccg ggtcgtcggg agccttgagc agcacgccgt cgctgacgtg tccgccacgg
     3121 ccgatccgat cgagctgaga tgcgagttct tcggcagttc cgctctcccg caacgagaag
     3181 cgcgctcgca cgagcgccgg ccgcaagctc gccaactgcg actcgaggga ggctcggacc
     3241 gcatcggaga accgcttcgg cgcctgcatg acgacgtcga tcaccaggga gatgccgtcg
     3301 agccgcagtt gcgtgtgttg tcggtccagc tcggcgatcg ctgcttcgac ctggcggcgg
     3361 gcctgggcgc tgacgtgcgg gcgctcgttg aggacgcgat cgaccgtcgc cacactcagc
     3421 cccgcctggg tggcgatgtc cgcgatccgg tgaccgtgcg ccataccccc tgactctaga
     3481 gagaaaggcc gactccagag agaacggccc gggcgggctg aggggaaatt gaggtgctga
     3541 ggtcaggaca ggctcaccaa accccgccat cctggaagca ggacaccaca gccgcacctg
     3601 tcaggagcct caccgatgag cgcactcgcc caagccgcaa ctcgccgcac gcccgccttc
     3661 cctgtcggaa ccctccacgc cggcgactgc cggctggcgg acctgctcga gatcctccag
     3721 gacaccaccg acctcgccga ctatccccac gcgagcggcg tggagcaggg cgtgctgctg
     3781 tatgccgccg cggacgttcg cgcggccctc gccgacggtg ccggagagga gctcgaggcc
     3841 gaactggcac gcgcctggtc gcaggggccc ggcatcatcg tcttcaccgg cgccttcgag
     3901 agcgagatca tcgaccgggt caacgcggca tacgaccgga tcatcaagca ggagaaggaa
     3961 tccggggggc ccaagggcga ccacttcggc gagcccggcg ccaacgaccg cgtctggaac
     4021 gccctggaga agctggcggt cgccgacccc gaggccttcg tcgactacta cagcaacgac
     4081 ctcgtcgcgc tcggcgcccg cgcgtggctc ggcccggcat accagatcac ggtgcagccc
     4141 aacgtcgttc gtcccggcgg caagggccag acggtgcacc gcgactacca cctcggtttc
     4201 atggattcgg agcaagcagc ccgcttcccg gcgcacgtgc acgccctctc ctccgtgctg
     4261 accctgcagg gcgccgtcgc gcacgtcgac atgcccgtcg agtccgggcc gacgctctac
     4321 ctcccgcaca gccagaaata cacccacggc tacctcgcct actggatccc ggagttccag
     4381 gactatttcg tcgccaacca catccagctg cccctcacca agggcgacct ggtctggttc
     4441 aacccggccc tgatgcacgc cgccggcacc aatgtctcgg cggacatcca gcgcatggcc
     4501 aacctgctgc aggtctcctc ggccttcggc cgcgcgatgg agagcgtcga ccgcgagcgc
     4561 gtggtcaacg ccgtctatcc ggcgctgctc gccgcgctcg acggcggtat gccgcggccc
     4621 ctggtcgaca acgccgtggc cgcgagcgcg gaaggctatg ccttcccgag caacctcgat
     4681 cgcgaccagc cggtcggcgg gatggcaccg cccacccagg ccgatctcgt gcacgccgcc
     4741 ctcgacacgc gcgagacacc cgactccctc cgcgatcggc tcgcccacca ggcatggacc
     4801 aagctcagcc actgactcct gcgccaccgg cacgggcatg ccgatgcttg tcggtcgcga
     4861 gcggcgcggc gagg
//
LOCUS       DAOPJG010000223         4839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_19036_length_4839_cov_8.350334, whole genome shotgun sequence.
ACCESSION   DAOPJG010000223 DAOPJG010000000
VERSION     DAOPJG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19036_length_4839_cov_8.350334"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKU75_15090"
     CDS             complement(<1..586)
                     /locus_tag="PKU75_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPF82251.1"
                     /translation="MRALTKVLAVTAVAGLALTGCGRSDSGSTGDSTSGSAAASGFDK
                     DAVIGISLPQKTSENWVLAEKLFNDGLTSAGFKGDVQFANGGVSEQQNQIQAMVTKGA
                     KVIVVGAIDGSQLGTQLKAAKESGATIIAYDRLLTNTADVDYYIAYDNFKVGQLQGQA
                     LLDGMMAKKPNGPYNIELFAGSPDDNNAKVFFDGA"
     gene            complement(651..1934)
                     /gene="gguB"
                     /locus_tag="PKU75_15095"
     CDS             complement(651..1934)
                     /gene="gguB"
                     /locus_tag="PKU75_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF82252.1"
                     /translation="MNAIKKIFGGDTRQLGMIFALIALIVFFQIKTGLTLDPNNVNNI
                     IQGNAYILVLAIGMVLVIIAGHIDLSVGSIAAFVGIVVAMGIRDWGIPWWLGILLGLV
                     IGALVGAWQGFWVSYLGIPAFIVTLAGMLIFRGANQWVGKSLTVPVPEAFQVIGAGSL
                     PEVGPNTGYNNLTILLGILGCAAIVFGAIRSRARLVKINAEVEEAWVMWTRLALICLV
                     IMGTMLLIASGRKGFPVSGLILVALVLIYGFISSKTVLGRHIYAVGGNRHAAELSGVK
                     SKKINFFVMCNMAVLSALAGMMFVARSTASGPFDGVGWELDAIAAVFIGGAAVTGGVG
                     TVIGSIVGGLVMAVLNNGLQILGVGADATSVIKGLVLLAAVLFDVYNKSQGKPSITGL
                     LMRNRKGSDSAAPAATNNPAADATSSLSETTRTEG"
     gene            complement(1954..3516)
                     /gene="gguA"
                     /locus_tag="PKU75_15100"
     CDS             complement(1954..3516)
                     /gene="gguA"
                     /locus_tag="PKU75_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPF82253.1"
                     /translation="MRNITKEFPGAKALSNVNMLVRRGEIHAICGENGAGKSTLMKVL
                     SGVWPVGSYDGEILFEGHPVTFQNINDSEKAGIVIIHQELALIPELSIAENIFLGNEQ
                     RKRGVIDWVLTQQRATELLARVGLSEDPNTKIKDIGVGKQQLVEIAKALSKDVKLLIL
                     DEPTSALNESDSQHLLSIMDGLRSKGVTCIMISHKLNEIEQISDSITIIRDGKSVETL
                     DVREDGVDENRIIRGMVGRDLESRFPDHTPQIGDVLFEIKDWNVQHATRTDRLVAKNS
                     AFTVRRGEIVGFAGLMGAGRTELMRSIFGRSYGTFLGGTLTLNGKERQLKSVSDAIDA
                     GIAYVTEDRKTLGLNLLDDIKTTTVAAKLKRITHNLVVSETEEFRYAEDYRKEMRTKT
                     PSVEEGVAKLSGGNQQKVVLAKWLFTEPELLILDEPTRGIDVGAKYEIYGIIQRLAEQ
                     GKGVIVVSSELPELLGLSDRIYTICEGAITGCLTKEEADQESLMRLMTKTSGAGGVAL
                     DEELAHDPGLSA"
     gene            complement(3673..>4839)
                     /locus_tag="PKU75_15105"
     CDS             complement(3673..>4839)
                     /locus_tag="PKU75_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPF82254.1"
                     /translation="PKAPVTRAQIAQATGLTRGTVSDLVESLVRGHMVTELAPRAAVG
                     AGRPGVPLAPASGTIVGIGAEVAVDRLGVTAIDLSGRAVNQRVVERDLRGSDPQRTLA
                     RLSAMVREVVGEVTGSGMTPAGACLALPGIVDASGMTRFAPNLGWQEVPALDLVRTGS
                     GLVDLSWSVGNDADLAARAECQARARAAEHAVADEDFVYVAGNVGLGGAVIRHGFLAG
                     GVHGWVGEIGHSPMDPLGPDCACGANGCLEQYAGRHIVMTQAGLAPTARIESLLEALG
                     DVPEGADEPARERHTKAVTAVDVAARAIGLGAATMVNIVDVEVVVLGSMYADLFAWVA
                     PTVQREVDRRVMAARWAPVRVERALVTELASLRGAALSVLEAVAAAPSSWTDRS"
BASE COUNT          736 a         1562 c         1724 g          817 t
ORIGIN      
        1 tggcgccgtc gaagaagacc ttggcgttgt tgtcgtccgg ggaaccggcg aagagctcga
       61 tgttgtaggg gccgttcggc ttcttggcca tcatgccgtc gaggagggcc tggccctgga
      121 gctgaccgac cttgaagttg tcgtaggcga tgtagtagtc gacgtccgcc gtgttggtga
      181 ggaggcggtc gtaggcgatg atcgtcgcgc cggactcctt ggcggccttg agctgggtgc
      241 ccagctgcga gccgtcgatg gcgccgacaa cgatgacctt ggcgcccttg gtgaccatgg
      301 cctggatctg gttctgctgc tccgagacgc cgccgttggc gaactgcacg tcgcccttga
      361 acccggccga ggtcaggccg tcgttgaaga gtttctccgc gaggacccag ttctccgagg
      421 tcttctgggg gagcgagata ccgatgacgg cgtccttgtc gaagccgctg gcggcagccg
      481 agcccgaggt gctgtccccg gttgaaccgc tgtcgctacg accgcagccc gtgagcgcca
      541 gacccgcgac cgccgtgacg gcgaggacct tggtcagtgc acgcatgctt tccttctctc
      601 tttcctgtgc cgtgccgggg tcccggtacg ggcgttggtg ggggagggac tcagccctcg
      661 gtgcgggtgg tctcggacag cgacgacgtc gcatccgcgg ccgggttgtt ggtggcagcc
      721 ggcgcggcgg agtccgagcc cttgcgattg cgcatcagca ggccggtgat cgacggcttg
      781 ccctggctct tgttgtagac gtcgaagagg acggcggcga gcaggaccag gcccttgatg
      841 accgacgtcg cgtcggcgcc cacgccgagg atctgcaggc cgttgttgag gacggccatg
      901 accagaccac ccacgatcga cccgatgacc gtgcccacgc caccggtgac ggccgcgcca
      961 ccgatgaaga cggcggcgat cgcgtcgagc tcccagccga caccgtcgaa ggggccggag
     1021 gcggtggacc gcgcgacgaa catcatgccg gcaagggccg agagaaccgc catgttgcac
     1081 atgacgaaga agttgatctt cttgctcttc acacccgaaa gctccgcggc gtggcggttg
     1141 ccgccgacgg cgtagatgtg gcggccgagg acggtcttgc tcgagatgaa tccgtagatg
     1201 aggacgagcg cgacgaggat caggcccgag acggggaagc cctttcgacc cgaggcgatg
     1261 agcagcatcg tgcccatgat gaccaggcag atgagggcca gccgggtcca catgacccag
     1321 gcctcctcga cctcggcgtt gatcttgacc agtcgcgccc ggctgcggat cgcgccgaac
     1381 acgatggcgg cacaccccag gatgccgagc aggatggtca ggttgttgta gccggtgttg
     1441 gggccgacct cggggaggga gccggcgccg atgacctgga aggcctcggg cacgggcacg
     1501 gtgagggact tgccgaccca ctggttggct ccccggaaga tgagcattcc ggcgagggtg
     1561 acgatgaagg ccgggatgcc gaggtaggag acccagaaac cctgccaggc gccgacgagg
     1621 gcgccgatga ccaggccgag caggatgccg agccaccagg ggatgcccca gtcgcggatc
     1681 cccatggcga cgacgatgcc gacgaaggcg gcgatcgacc cgacggagag gtcgatgtgg
     1741 ccggcgatga tgacgagcac catgccgatg gccagcacca ggatgtaggc gttgccctgg
     1801 atgatgttgt tgacgttgtt ggggtccagg gtcagaccgg tcttgatctg gaagaacacg
     1861 atgagcgcga tgagcgcgaa gatcatgccg agctggcggg tgtcgccgcc gaagatcttc
     1921 ttgatggcat tcatgtcagg tccttcgtcg tggtcaggcc gacaggccgg ggtcgtgggc
     1981 gagttcctcg tcgagggcga cgcccccggc tccggaggtc ttggtcatca gccgcatgag
     2041 cgactcctgg tcggcttcct ccttggtcag gcagccggtg atggctccct cacagatcgt
     2101 gtagatgcgg tccgagagac ccagcagctc cgggagctcg gacgagacga caatgacacc
     2161 cttcccctgc tcggccaggc gctggatgat cccgtagatc tcgtacttgg cccccacgtc
     2221 gatgccgcgg gtgggctcgt cgaggatgag cagctcgggc tcggtgaaga gccacttggc
     2281 caggacgacc ttctgctggt tgccaccgga gagcttggcc acgccttcct cgacgctcgg
     2341 cgtcttggtg cgcatctcct tgcggtaatc ctcggcgtag cggaactcct cggtctcgct
     2401 gacgaccagg ttgtgggtga tccgcttgag cttggcggcc accgtcgtgg tcttgatgtc
     2461 gtcgaggagg ttcagcccaa gggtcttgcg gtcctcggtg acataggcga tgccggcgtc
     2521 gatcgcgtcg gagaccgact tgagctggcg ttccttgccg ttgagcgtga gggtgccgcc
     2581 gaggaaggtg ccataggagc ggccgaagat cgagcgcatg agctcggtgc ggcccgcgcc
     2641 catgaggccg gcgaagccga cgatctcgcc ccgccgcacg gtgaaggcgc tgttcttggc
     2701 caccagcctg tcggtgcggg tggcgtgctg gacgttccag tccttgatct cgaagagcac
     2761 gtccccgatc tgcggggtgt ggtcggggaa gcgcgactcc aggtcgcggc cgaccatgcc
     2821 ccggatgatg cggttctcgt cgaccccgtc ttcacgcacg tcgagggtct cgaccgactt
     2881 gccgtcgcgg atgatggtga tggagtccga gatctgctcg atctcgttga gcttgtggct
     2941 gatcatgatg caggtgacgc ccttgctgcg caggccgtcc atgatcgaga gcaggtgctg
     3001 ggagtcgctc tcgttgagcg ccgaggtggg ctcgtcgagg atgaggagct tgacgtcctt
     3061 ggacagcgcc ttggcgatct cgacgagctg ctgcttgccg accccgatgt ccttgatctt
     3121 ggtgttgggg tcctcgctca ggcccacccg ggcgaggagc tcggtggcgc gctgctgggt
     3181 gaggacccag tcgatgacgc cgcgcttgcg ctgctcgttg cccaggaaga tgttctcggc
     3241 aatggagagc tcggggatga gcgccagctc ctggtgaatg atgacgatcc cggccttctc
     3301 ggagtcgttg atgttctgga aggtcaccgg gtggccctcg aacaggatct cgccgtcata
     3361 gctgccgacc ggccagaccc ccgagaggac cttcatcaag gtcgacttcc ccgcgccgtt
     3421 ctcgccgcag attgcgtgga tctcaccgcg gcggacgagc atgttgacgt tgctgagcgc
     3481 cttggcgccg gggaactcct tggtgatgtt ccgcatctcc aggatgacct gccctgcggg
     3541 cacgcttctc cccacttgct ccgaagccgc caccggcgta cctccccgtc gaggaattgt
     3601 tgcgggggtg aacttattcc gaagtccgcg tgtgcgcaag acgaacacgc tccgtctcgc
     3661 gctacggaat ggtcacgatc tgtccgtcca gctgctgggc gccgcggcga ccgcctcaag
     3721 cacgctcagg gccgcgcccc gcaggctcgc cagctccgtc accagggcgc gctcgacccg
     3781 gaccggggcc cagcgggccg ccatcacgcg ccggtcgacc tcgcgctgca cggtgggcgc
     3841 cacccaggcg aagaggtcgg catacatgct gcccaggacc acgacctcga cgtcgacgat
     3901 gttgaccatc gtcgccgcgc ccaggccgat ggcgcgggcc gcgacgtcga ctgcggtcac
     3961 agccttggta tggcgctccc gcgcgggctc gtcggcgccc tccggcacgt cgcccaatgc
     4021 ctccagcagc gactcgatgc gagccgtggg ggccaggccg gcctgggtca tcacgatgtg
     4081 gcgcccggcg tactgctcga ggcagccgtt ggcgccgcac gcgcagtccg ggccgagcgg
     4141 gtccatgggg gagtggccga tctcgccgac ccaaccgtgg acgccgccgg ccaggaaccc
     4201 atgacggatg accgcgcccc cgagcccgac attgcccgcg acatagacga agtcctcgtc
     4261 ggcgaccgcg tgctccgcgg cgcgggcgcg ggcctggcac tcggcacggg cggcgaggtc
     4321 ggcgtcattg ccgaccgacc aggagaggtc gaccaggccg ctcccggtcc gcaccaggtc
     4381 gagcgcgggc acctcctgcc agcccaggtt gggggcgaag cgggtcatcc cggaggcgtc
     4441 gacgatgccg ggcagggcca ggcaggcgcc tgccggcgtc ataccgctgc cggtcacctc
     4501 gcccaccacc tcacgcacca tggcggagag ccgggcgagg gtgcgctgcg ggtcgctgcc
     4561 gcgcaggtcg cgctccacga cgcgctggtt gaccgcccgt ccgctgaggt cgatggcggt
     4621 caccccgagc cggtcgaccg ccacctccgc cccgatcccg acgatcgtgc cgctcgccgg
     4681 ggccagtggc actccgggcc gcccggcgcc gaccgcggcg cgtggggcga gctcggtgac
     4741 catgtggccg cgcacgaggg actcgacgag gtcggagacg gtgcctcgcg tcaggccggt
     4801 cgcctgggcg atctgggcgc gcgtgacggg cgccttcgg
//
LOCUS       DAOPJG010000224         4837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_19047_length_4837_cov_8.041196, whole genome shotgun sequence.
ACCESSION   DAOPJG010000224 DAOPJG010000000
VERSION     DAOPJG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19047_length_4837_cov_8.041196"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..899)
                     /locus_tag="PKU75_15110"
     CDS             complement(144..899)
                     /locus_tag="PKU75_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF82255.1"
                     /translation="MSSLFDIAGRRALVTGSSRGIGFALARALAEAGAEVILNARDAA
                     ALDAARTRLQQVASAPVHTAPFDVTDEDDVRRAIGDLGAVDILVNNAGMQRRAPFPDF
                     ATTDFDALLATNVRSVFLVSREVSRAMIERGAGKIITICSVQSELARPGIAPYSATKG
                     AVRMLVKGMCADLGPHGIQVNGLAPGYFSTELTQALVADEQFTEWVRTRTPAGRWGQV
                     EDLAGTLLWLASPASDFVNGQTIFVDGGMTAVL"
     gene            complement(947..2044)
                     /locus_tag="PKU75_15115"
     CDS             complement(947..2044)
                     /locus_tag="PKU75_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPF82256.1"
                     /translation="MRIRGAVLTEIGKPGPYAVTRPLVITDLDLAPPGPTEVLVEVDA
                     AGLCHSDLSVVDGNRVRPVPMLLGHEGSGIALEVGRAVEGIAPGDRVVMTFLPRCGDC
                     AGCATDGIRPCVPGSARNGAGEMLTGGRRLSLDGAPVNHHLGVSAFATHAVLDARSLV
                     VVEPDVPPTVAALLGCAVLTGGGALLNAGRPRPGERVAVVGLGGVGMAALLTGAGLDD
                     VEVTGIDLVASKRDLGSDLGAHASMAPEEAPDAGFDVVIEGAGSGRAFETAYRLLAPG
                     GRMVTVGLPAPDATATITPLDLVAGGRTIVGSYLGSAVPARDVPRFAQLWREGRLPVE
                     RLVSHELGLDDVNAGMDRLARGEAVRQIVRF"
     gene            complement(2041..2481)
                     /locus_tag="PKU75_15120"
     CDS             complement(2041..2481)
                     /locus_tag="PKU75_15120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPF82257.1"
                     /translation="MTTVPTPLVRDFPVRWPVQTRWTDNDMFGHLNNAVYYAMWDSAI
                     NAWLAQQTGADPMKDAAFPVVAESSCRYVSELGYPDDLVAGIGVERLGSSSVTLRLAL
                     YCASATDADPVAAVGRWVHVYVDRATRKPTPMPAGERAAYEAIR"
     gene            complement(2583..>2769)
                     /locus_tag="PKU75_15125"
     CDS             complement(2583..>2769)
                     /locus_tag="PKU75_15125"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HPF82258.1"
                     /translation="VGMPRSGRWRVRVNTDAPMFGEGFGSVHAADVDTVPEWADGCPQ
                     RVSLTIGPYAALVLSQD"
     assembly_gap    2770..2869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2870..4507)
                     /locus_tag="PKU75_15130"
     CDS             complement(<2870..4507)
                     /locus_tag="PKU75_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPF82259.1"
                     /translation="MPHDGGVTFRVWAPNARHVAVIGDFCDWQLPHKVPLARDHARAG
                     TWSVFVPHAGPGSEYRFLIRRGGPYVWRIDPCARQVTHSRGNGVVVDPATLGFDGHHF
                     DAPAWDDLVVYETHIPTFAGNASGRGTFEQAIGRLDHLAWLGVSAIEVMPPFEFGTPI
                     SWGYNPSHLFAIESSYGGPHAFARFVREAHARGIAVILDVVHNHLGPTNLDLWRFDGG
                     GSGRYGGIYFYNDRRAHTPWGATRPNFSKKEVRNFLRDSAVQWLETFQVDGLRFDGTN
                     FIRSRLGNVFEPQDQLAPGHAFLAAMTADLRSRQPWKLLIAEDMQRDGIVTRPSADGG
                     LGFHSQWDAGFVHPVRRALECPRDEDRDLEAVAAAVTGFGLGSAYERVIFTESHDEVA
                     NGKSRVPESIDPGAADSWHARKRAALGLALTLTSPGIPMLFQGQDFLEDRWFDDTVVL
                     DWDKAHLHSGFLHLVRELIHLRRNRFGVTAGLTGTNTRVLRLDQHAKVLAFHRWRHGG
                     PRDDTVVVVNLSTHPLHDYGVGMPRSGRWRVRVNTDAP"
     gene            complement(4654..>4837)
                     /locus_tag="PKU75_15135"
     CDS             complement(4654..>4837)
                     /locus_tag="PKU75_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82260.1"
                     /translation="VGARGMGHDRRARRHDGPLVRSAAQPAAAPLVTGIHVFTFNEFT
                     GAEEWRLKLRRDVGLG"
BASE COUNT          727 a         1597 c         1736 g          677 t
ORIGIN      
        1 ttgacgatgt cggtcatccc ccgattctgc ctgacgcaga cgccttggtg gaggcggggc
       61 ggcgtgagcc ggggagagta gcgccacggc gatccgtggg cccgcacgcc gtccgggacc
      121 tcacctcgtg ggcctgatcg ccgtcacagg acggctgtca tccctccgtc gacgaagatc
      181 gtctggccgt tgacgaagtc ggatgccggg ctggcgagcc acagcagggt tcccgcgagg
      241 tcttcgacct gtccccaacg gcctgcgggc gtccgggtgc gcacccattc ggtgaactgt
      301 tcgtcggcga ccagggcctg ggtcagttcg gtcgagaagt agcccggcgc aaggccgttc
      361 acctgtatgc cgtggggccc caggtcggcg cacatgccct tgacgagcat ccgcacagcg
      421 cccttcgtcg cgctgtacgg cgcgatcccg gggcgggcga gctccgactg caccgaacag
      481 atcgtgatga tcttgccggc accgcgctcg atcatggcac ggctcacctc ccgcgagacg
      541 aggaagaccg agcggacgtt ggtggccagg agggcgtcga agtcggtcgt cgcgaagtcg
      601 gggaagggcg cgcgtcgctg catgcctgcg ttgttgacga ggatgtcgac ggcacccagg
      661 tcaccgatgg cccgccgcac gtcgtcctcg tcggtgacat cgaagggcgc cgtatgcacg
      721 ggtgcggagg caacctgctg cagccgggtg cgcgccgcgt cgagggcggc ggcgtcccgg
      781 gcattgagga tcacctccgc gcccgcctcg gccagggcgc gcgccagtgc gaacccgatg
      841 ccccggctcg atccggtgac cagggcgcgg cggccggcga tgtcgaagag tgaactcacc
      901 gcctcatcat ggccgacgcg gcagatccgg cgtgctctcc gaaccctcag aagcggacga
      961 tctggcggac cgcttccccc cgggccagcc ggtccatgcc ggcgttgacg tcgtcgagcc
     1021 ccaactcgtg cgagacgaga cgctcgacgg ggagccggcc ctcgcgccac agctgggcga
     1081 accgcgggac gtcccgcgcc ggcacggctg agccgaggta gctaccgacg atcgtgcggc
     1141 ctcccgccac gagatcgagg ggcgtgatcg tcgccgtcgc gtcgggggcg ggcaggccga
     1201 cagtgaccat ccggccgccg ggggcgagca gacggtatgc cgtctcgaac gcccgcccgc
     1261 tgcccgcccc ctcgatcacg acgtcgaatc cggcgtcggg tgcctcctcc ggtgccatcg
     1321 aggcgtgggc gccgaggtcg ctgcccaggt cgcgcttcga cgcgacgagg tcgatgccgg
     1381 tcacctcgac gtcgtcgagg ccggcgccgg tcagcagggc cgccatgccg acgccgccca
     1441 ggccgaccac ggccacccgc tcacccgggc ggggccggcc cgcattgaga agggcgccgc
     1501 cgccggtgag gaccgcgcag ccgagaaggg ccgccacggt cggcggcacg tcgggctcga
     1561 ccaccacgag cgagcgggcg tcgagcaccg cgtgcgtggc gaatgccgag accccgagat
     1621 gatggttgac cggggcaccg tcaaggctca gtcgccttcc gcccgtgagc atttcgcccg
     1681 caccgttgcg ggccgagccg ggcacgcagg gccggatgcc gtcggtggcg cagccggcac
     1741 agtcaccgca gcggggcagg aaggtcatca cgacccggtc gccgggcgcg atgccctcga
     1801 ccgcccgccc gacctccagc gcgatcccgc tgccctcgtg gccgagcagc atcgggacgg
     1861 gccgcacgcg gttgccgtcg acgaccgaca ggtcggagtg acacaggccg gcggcgtcga
     1921 cctcgacgag cacttccgtc ggccccggcg gcgcgagatc gaggtcggtg atcacgagag
     1981 ggcgggtcac ggcatacggt ccgggcttgc cgatctcggt gaggacagca ccgcggattc
     2041 tcatcgaatc gcctcgtatg ccgcgcgctc gcccgccggc atgggcgtgg gcttgcgggt
     2101 ggcccggtcg acgtagacat ggacccaacg gcccacggcc gcaacgggat cggcgtctgt
     2161 tgcgctggcg caatacagtg ccaggcgcag cgtgacggag gaggagccga ggcgctcgac
     2221 gccgatgccg gcgaccagat cgtccggata gccgagctcg ctcacatagc gacaagaaga
     2281 ctcggcgacg acggggaagg ccgcatcctt catgggatcg gctccggtct gctgcgcgag
     2341 ccaggcgttg atcgcgctgt cccacatggc gtaatagacg gcgttgttga gatggccgaa
     2401 catgtcgttg tcggtccagc gggtctggac cggccagcgc accgggaagt ccctgacgag
     2461 cggggttgga acggtcgtca caggtggatc attccactcg ggtggggtga tggaggcgaa
     2521 tcccggctac ggccgcgatt ggatatcgcc actccggcga cgccgccggt ccggggaacg
     2581 cttcagtcct gcgagaggac cagggcggca tacggcccga tggtcagtga cacccgctgg
     2641 gggcagccgt cggcccactc gggcaccgtg tcgacatcgg cggcgtgcac ggacccgaaa
     2701 ccctcgccga acatcggggc gtcggtgttg acgcgcacgc gccagcggcc ggaccgcggc
     2761 atacccaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggggcgtcgg
     2881 tgttgacgcg cacgcgccag cggccggacc gcggcatacc caccccgtag tcgtgcagtg
     2941 ggtgcgtgga gaggttgacc acgacgacgg tgtcgtcgcg agggccgccg tgccgccacc
     3001 ggtggaaggc gaggaccttc gcgtgctgat cgaggcgcag cacccgggtg ttcgtccccg
     3061 tcaaacccgc cgtgacgccg aacctgttgc ggcgcaggtg gatcaactcg cgcacgaggt
     3121 ggaggaaacc gctgtgtagg tgggccttgt cccaatcgag cacgacagtg tcgtcgaacc
     3181 agcggtcctc gaggaagtcc tgcccctgga agagcatggg gatccccggc gacgtgagag
     3241 tgagggccag tccgagcgcc gcgcgcttgc gggcgtgcca ggagtccgcg gccccgggat
     3301 cgatggactc cggcacccgg gacttcccgt tggccacctc gtcgtggctc tccgtgaaga
     3361 tgacccgctc gtatgccgag ccgagcccga agccggtgac cgctgcggct acggcctcga
     3421 ggtcgcggtc ctcgtcgcgc gggcactcca gtgcccttcg cacagggtgg acgaatccgg
     3481 cgtcccactg actgtgaaac cccaggccac cgtcagcact cgggcgggtg acgatcccat
     3541 cgcgttgcat gtcctcggcg atgaggagtt tccacggctg ccgggagcgc aggtccgccg
     3601 tcatcgccgc gaggaaggca tgcccggggg ccagctgatc ctggggttcg aagacattgc
     3661 ccaaccgcga gcggatgaag ttggtgccgt cgaatcgcag cccgtcgacc tggaaggtct
     3721 ctagccactg cacggcgctg tcgcgcagga agttgcggac ttccttcttg ctgaagttgg
     3781 gtcgcgttgc gccccagggc gtatgggcgc gccggtcgtt gtagaagtag atccccccgt
     3841 agcgcccgct gccgccgccg tcgaaacgcc acaggtcgag attcgtcggc ccgagatggt
     3901 tgtggacgac gtcgaggatg acggcgatgc cgcgcgcgtg agcctcccgc acgaaacggg
     3961 cgaaggcgtg cgggccgccg tagctgctct cgatggcgaa gaggtgagag gggttgtagc
     4021 cccagctgat cggcgtgccg aattcgaagg gcggcatcac ttcgatggca ctgacgccga
     4081 gccaggcgag atggtcgagc cgtccgatcg cttgctcgaa tgtccctcgg ccactggcgt
     4141 ttccggcgaa ggtggggatg tgcgtctcgt agacgacgag gtcgtcccag gcgggtgcgt
     4201 cgaagtggtg gccgtcgaag cccagcgtgg ccgggtcgac gacgacgcca ttgccgcggg
     4261 agtgggtgac ctgcctggcg caggggtcga tgcgccagac ataggggccg ccgcggcgga
     4321 tgaggaagcg gtattcgctg cccggccccg cgtgcgggac gaagaccgac cacgtgccgg
     4381 cgcgggcatg atcgcgcgcc aggggcacct tgtgcggcag ctgccagtcg cagaagtcgc
     4441 cgatgaccgc gacgtggcga gcattcggtg cccagacccg gaaggtcacg ccgccgtcgt
     4501 ggggcacggc gcccattcca gggtgccccg acgctcgagc tctcgcgtgc gtggagcccc
     4561 gcagcgagga cggaccggac cgggcaggca caaggtctta ccctacgtcg ccgcgcgccg
     4621 agcagccgca tgccgcggcg taacccgccg tcgtcagccg aggccgacgt cgcgacgcag
     4681 cttgaggcgc cactcctcgg caccggtgaa ctcgttgaag gtgaagacgt ggatgccggt
     4741 gacgagcggt gcggcggcgg gctgggcggc ggatcgaact aaaggtccat cgtgtcgtcg
     4801 ggctcgtcgg tcgtgcccca tcccgcgagc tcccacg
//
LOCUS       DAOPJG010000225         4713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_19757_length_4713_cov_7.211464, whole genome shotgun sequence.
ACCESSION   DAOPJG010000225 DAOPJG010000000
VERSION     DAOPJG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19757_length_4713_cov_7.211464"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1880
                     /gene="valS"
                     /locus_tag="PKU75_15140"
                     /pseudo
     CDS             1..1880
                     /gene="valS"
                     /locus_tag="PKU75_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884182.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
     gene            1922..2635
                     /locus_tag="PKU75_15145"
     CDS             1922..2635
                     /locus_tag="PKU75_15145"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HPF82261.1"
                     /translation="MDVRAPASRHAPVALVLAVVLGLAMFVAGSGPVFVVGDAYESRL
                     EPSVSTTTPSPTSVVDPAAPTPPPPIPSPPEFATNGWVRLILGVLAGALVLAVLWRLF
                     LMWRGRPREIPAPDVPPVEPVAEAVLDDADEQFAALRSGAPNNAIVTCWQRLEAAIDR
                     AGCPALPWETPAEVTSAVLRRFEIDDDAIAGLADLYREARFSRHALGEADRERAVDAL
                     TRIHEGLAHARVPEAEQAP"
     gene            2632..3150
                     /locus_tag="PKU75_15150"
     CDS             2632..3150
                     /locus_tag="PKU75_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82262.1"
                     /translation="MTRSRIPTWPAGPGIPLISFGVLRLLIPVILVALGIAWYSFTPA
                     PNAVLFCSILLAGWVIGWVVYRLARLGTPQRWQVFRPYPPRHRGRDSRVSALRVLLDE
                     PRRENDEELHRIISAIVSDRLSQRRVHGQAENDPGALLGGELAAYLNAPEGMRRRPIH
                     TIDTMLQRIERL"
     gene            3151..4104
                     /locus_tag="PKU75_15155"
     CDS             3151..4104
                     /locus_tag="PKU75_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPF82263.1"
                     /translation="MDLPTTSRLGGEILDEVERAVVGKRDALTLLLAGVLAGGHVLLE
                     DFPGLGKTLAARSLATALGLDFKRAQFTPDLLPADLTGSFVFDQRTATFEFRHGPIFA
                     GLLLADEINRTPPKTQAALLEAMQEHQVTVEGRTFPLPKPFHVLATANPIEYEGTYPL
                     PEAQLDRFLMRLEFGYPSPTEEWQVLTARMGRRTEEHSLRAVTDPTGLLAMQQAIETV
                     TVDDSVSQYCVRLAGSTRRHAQVLVGASPRGSLALLLCSRAYAVLAGRDYVTPEDVKA
                     VAHPVLDHRVSVRPELWASDVTASAIVEDILRSVPTPASGP"
     gene            4101..>4713
                     /locus_tag="PKU75_15160"
     CDS             4101..>4713
                     /locus_tag="PKU75_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82264.1"
                     /translation="MSGSRWEPTAAHVRALVVALVVGLGAALARRPDLLVLASPFVIA
                     AAWGLAGRPDGLPQARHQVSPAVVPEGGIVHWEAHITVPRGARDLAAVQAPTAYFASL
                     EGAGAVAVAVPDTHLPDTPVALQDNWIAERWGHYDTGSAIVTVTSALGAFRWSPGQLP
                     DTRVVVLPGKDAFTSRTGMPHPTGIVGLNRGTRSGDGTDFAAVR"
BASE COUNT          666 a         1769 c         1598 g          680 t
ORIGIN      
        1 cgacgcgccc cgagctgatc ccgtccgtgg tgccgatgcg cgccccttcc cacgcctagc
       61 ggtatgccgc gcgcttcggc accacggtca cctcgccgtt gttcggggtc gagatccccg
      121 tcgtcgcgca ccccgccgcc gagatggaca agggcgcggg catcgcgatg tgctgcacct
      181 tcggtgacct caccgacgtg ctgtggtggc gcgagctgca gctgcccacc cgctcgctga
      241 tcacccgcac gggccggatg caccacgaga acccggagtg gctcgtcggc acccccggcg
      301 agcagctgta tgccgagcag atcgccggca agaccgcata cggcgcccgc gaggcgatcg
      361 tggcgctgct gcgcgaatcc ggcgacctcg acggcgagcc cacgccgacc cagcgcaagg
      421 cgaacttcta cgagcgcggc gagaagccgc tggagatcgt caccacccgc cagtggtata
      481 tccgcaacgg gggccgcgac gccgccctca acgccgagct cgtggcccgc ggcgacgaga
      541 tcgccttcca cccggccttc atgcgcagcc gttatgccaa ctgggtcgcc ggcctcaacg
      601 gcgactggct ggtgtcgcgg cagcgctttt tcggaccgac catccccgtc tggtatcccc
      661 tcgacgagga cggagaaccg ttgtgggaca actatattct gcccaccgag gagatgctgc
      721 ccatcgatcc gatggccgac gcaccccccg gttacaccga ggaccagcgc ggcaagccgg
      781 gcggtttcgt cggtgacccg gacgtcttcg acacctgggc cacgtcttcg ctcaccccgc
      841 agatcgccct cggctggcgc cgcgacgacg aactcttcgg ccacctcttc ccgatggacc
      901 tgcgcccgca ggcacacgac atcatccgca cctggctctt cgcgaccgtc gtgcgtgcgc
      961 actgggagca cggcacgatc ccgtggaccg acgccgcgat cagcggcttc atcctcgacc
     1021 ccgaccgcaa gaagatgagc aagtccaagg gcaatgtggt cacgcccgag gacatcctca
     1081 aggagcacag caccgacgcg gtgcgctact gggcggccag cggccggctc ggcaccgacg
     1141 cggcatacga cgtgggccag atgaaggtcg ggcgccgcct ggcgatcaag ctgctcaacg
     1201 ccacgaagtt cgcactgtcg ttcggggagg tggaggcgtt cggtgaggtg gagggcgacc
     1261 tgcgcgcggc cgtcaccgag cccctcgacc tcgccatgct cgccggcctg cgcgaggtcg
     1321 tcgagaaggc gaccgcgggc ttcgagtcat gggactacac gcgcagcctg gagatcaccg
     1381 aggcgttctt ctggaccttc tgcgacgact acctggagtt ggtcaaggac cgcgcctacg
     1441 gcggccaggg cgagggtccg gcagcaagcg cccgcgcggc cctgcggctc gggctcgacg
     1501 tgctgctgcg gctgctcgcc ccgatccagc cctatacgac cgaagaggtg tggtcgtggt
     1561 ggcacgaggg gtcgatccac cgggcctcgt ggccgacggc cgaggagttg ccggaaggcg
     1621 gcgacgcgac gatgctcgcc tccgtgggcg ccgcgctcgc cgcggtgcgc aaggcgaagt
     1681 cggaggccaa ggtcggtatg cggtccgaga tcaccgcgtt gaccctcacc gggccggacg
     1741 cggcgttgga gcagatccgc gccgccgcgg acgacctgcg ggcggcgggg cggatcaccg
     1801 ccgaaccgca gtatgccgcg ggcgaggcga tcgccgccgc ggacgtcgag ctcatccccg
     1861 ccgagaagcg cgccggatag tctcgcctcg agtgccatcg gcacgcagag gagggcacgt
     1921 tatggacgtc cgggcacccg cgagccgcca cgcgccagtg gcgctggtgc tcgccgtggt
     1981 gctcggactg gcgatgttcg tcgccgggtc ggggccggtg ttcgtcgtgg gcgatgcata
     2041 cgaaagccgg ctcgagccgt cggtgtccac caccacgccg tccccgacgt cggtcgtcga
     2101 tccggccgca cccaccccgc cgcccccgat cccctcgccg ccggagttcg ccaccaacgg
     2161 ctgggtgcgg ctgatcctcg gggtcctcgc cggcgccctc gtcctcgcgg tgttgtggcg
     2221 actgttcctc atgtggcgcg ggcgaccgcg cgagatcccg gcgcccgatg ttccccccgt
     2281 ggaaccggtg gcggaggcgg tgctcgacga cgccgacgaa cagttcgccg ccctgcgctc
     2341 gggagcgccc aacaacgcca tcgtcacgtg ctggcaacgt ctcgaagccg ccatcgaccg
     2401 ggccggctgt ccggcgttgc cgtgggagac ccccgccgag gtgaccagcg cggtcctgcg
     2461 gcgcttcgag atcgacgatg acgccatcgc tgggctggcc gacctctacc gggaggcacg
     2521 gttcagccgg cacgccctcg gcgaagccga ccgggagcgc gctgtcgacg ccctgacccg
     2581 gatccacgag ggcctcgccc acgcacgtgt cccggaagcg gagcaggcgc cgtgacgcgg
     2641 tcccggatcc ccacctggcc cgccgggccg ggcataccgc tgatctcctt cggggtgctg
     2701 cggttgctca tccccgtgat cctcgtcgcg ctcggcatcg cgtggtactc cttcaccccg
     2761 gcgcccaacg ccgtgctgtt ctgctcgatc ctgttggcgg gctgggtgat cggctgggtc
     2821 gtctatcgcc tggcccggct ggggacgccg caacgctggc aggtcttccg gccctacccc
     2881 ccgcggcacc gtggccggga ctcgcgggtc tcggccctgc gggtgctcct cgacgagccc
     2941 cggcgcgaga acgacgagga actgcaccgc atcatctcgg cgatcgtgag cgaccggctc
     3001 agccagcgcc gggtccacgg ccaggctgag aacgacccgg gcgccctcct cgggggcgag
     3061 ctcgcggcat acctcaatgc gccggaaggt atgcggcgca ggccgatcca caccatcgac
     3121 accatgctcc agcgcatcga gaggctctga atggacctgc ccaccacctc ccgcctgggc
     3181 ggcgagatcc tcgacgaggt cgagcgagcc gtcgtcggca agcgcgacgc cctcaccctt
     3241 ctgctcgccg gcgtcctcgc cggcgggcac gtgttgttgg aggacttccc cggtctcggc
     3301 aagaccctcg ccgcacgcag tctcgcgacc gcgctcgggc tggatttcaa gcgggcacag
     3361 ttcactccgg acctgctgcc ggccgatctg acgggctcct tcgtcttcga tcagcgcacc
     3421 gcgaccttcg agttccggca cggcccgatc ttcgccgggc tcctcctcgc cgacgagatc
     3481 aaccgcaccc ctcccaagac ccaggcggcc ctgctggagg cgatgcagga gcaccaggtc
     3541 acggtcgagg ggcgaacctt cccgctcccc aagccttttc acgtcctcgc gaccgccaac
     3601 cccatcgagt acgaaggcac ctacccactg cccgaggccc agctcgaccg cttcctgatg
     3661 cgcctggagt tcggctatcc gagcccgacc gaggagtggc aggtcctcac cgcgcggatg
     3721 ggccggcgca ccgaggaaca cagtctgcgc gccgtcaccg acccgacggg cctcctcgcc
     3781 atgcagcagg cgatcgagac ggtgacggtg gacgactcgg tgagccagta ctgcgtccgg
     3841 ctcgccggct cgactcgccg gcacgcccag gtcctcgtgg gcgcctcgcc ccgtggctcg
     3901 ctggctctgc tgctgtgctc tcgtgcgtat gccgtgctgg ccggccgcga ctatgtcacc
     3961 cccgaggacg tcaaggccgt ggcgcacccc gtgctcgacc accgcgtgag cgtgcggccg
     4021 gagttgtggg ccagcgatgt caccgcgtcc gcgatcgtcg aggacatcct gcggtcggtc
     4081 ccgaccccgg cgagcggccc atgagcggga gccgctggga gccgacggcg gcacacgtcc
     4141 gggccctcgt cgtcgccctg gtcgtcggtc tcggcgccgc gttggcgcgc cggccggacc
     4201 tgcttgtcct cgcctcgccc ttcgtcatcg ccgccgcgtg gggcctggcg gggcgaccgg
     4261 acggcctccc ccaggcccgg catcaggtgt cgccggccgt cgtgcccgag ggcggcatcg
     4321 tgcactggga ggcacacatc accgtgccgc gcggagcccg cgacctggcc gcggtccagg
     4381 caccgacggc gtacttcgcc agcctcgagg gcgccggcgc ggtcgccgtc gccgtgcccg
     4441 acactcacct gccggacacg ccggtagcgc tgcaggacaa ctggatcgcc gagcggtggg
     4501 gacactacga cacgggttcc gcgatcgtca ccgtcaccag cgcgctcggt gccttccggt
     4561 ggagcccggg ccagttgccc gacacccgcg tcgtggtgct gcccggcaag gacgccttca
     4621 ccagccgcac cggcatgcca caccccaccg gcatcgtggg gctcaaccga ggcacccgaa
     4681 gcggcgacgg caccgacttc gccgccgtgc ggg
//
LOCUS       DAOPJG010000226         4707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_19793_length_4707_cov_7.103396, whole genome shotgun sequence.
ACCESSION   DAOPJG010000226 DAOPJG010000000
VERSION     DAOPJG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19793_length_4707_cov_7.103396"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKU75_15165"
     CDS             complement(<1..628)
                     /locus_tag="PKU75_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPF82265.1"
                     /translation="MTADSTTAGGGGQVLTAATVLTPDAVLSPGWVRVEGDRITEVGD
                     GLPPGPTGSAGRDEWVATDLGAATLAPGFVDVHSHGGGGAAYTDGVEAAGIARAAHLA
                     HGATSTMASLVTDTVDRLVDQVRALAPLVACDDLLGVHLEGPWLAERYKGAHDPGLLR
                     APSPEDVDRLIAAGPVAMVTLAPELPGASEAVRRFVEAGVLVAIGHSDA"
     gene            complement(625..771)
                     /locus_tag="PKU75_15170"
     CDS             complement(625..771)
                     /locus_tag="PKU75_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82266.1"
                     /translation="MAPTPAGLAGVAGFQRTPSDAAYAHPTEGRNRRALEIAIITADL
                     AAPT"
     gene            799..1887
                     /locus_tag="PKU75_15175"
     CDS             799..1887
                     /locus_tag="PKU75_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPF82267.1"
                     /translation="MLVAELERIIPIIVVLLAAAVLAELSDRAGVFDVVGHWLARHAR
                     HRLLVLWLLFSAYAVICTVFLSLDTTAVLLTPVALAIAKQVGVPARPFALTTLWIANT
                     GSMLLPVSNLTNLLAQSKFRALGVDHTAYLRLALVPGLVCIVVTLVVILALHPRVLTT
                     QYTVDPPAEPHDQVLLRVGLVTCLLVGPAFAAGLAPWAVAGVAAGVLLLVTWLRAPHL
                     LRGLAVPWVMAAGFAVLALLVGWAQHAGHLAWLGQLVGQGTGLTDLLRVTGTAALTSN
                     LVNNLPAYLAVEPFAADHPARLMAALVGANAGPLVTPWASLATLLWLQRCRAGGLRWR
                     LGRLALAGLGCALASTVLAVLALALVTG"
     gene            1918..2469
                     /locus_tag="PKU75_15180"
     CDS             1918..2469
                     /locus_tag="PKU75_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPF82268.1"
                     /translation="MAVLSFSMNQSLDGNVAHDAFAPGPLMFAHFIDQVAGLSGSIYG
                     RTMYEVMRYWDEDQPDWVDAEKAYAEVWRRQPKWVVSRTLDSVGPNATLVRGDVASEV
                     ARIRAEHDGRIEVAGPTLAAGLGALGLIDEYVIYLHPVVAGPGPTFFGDDRPRLELVA
                     AEPLAEGVVKLTYAPAGNEPALP"
     gene            2676..3296
                     /locus_tag="PKU75_15185"
     CDS             2676..3296
                     /locus_tag="PKU75_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPF82269.1"
                     /translation="MTSFLIPRPTQGDVVELILDDHRAFEDLLRECRRSDRDRATARA
                     ALAELLVAHAEAEESEVYPTLRTKRAISAHKEEHGEEEHAEIVEALLAFLDAKGTSTK
                     KYDEALEELSKVVSHHNCEEELDILNPARDDVSVAVRHELGVKWARKRNALLKAGCAS
                     RTQVAALLKEAVKDKTLPPEPVREQMDAIKERAKKKADALGEEATS"
     gene            3502..4500
                     /locus_tag="PKU75_15190"
     CDS             3502..4500
                     /locus_tag="PKU75_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPF82270.1"
                     /translation="MLYPATPREVGSTATIADPTLWVLTVAGVLALLALDFLITRRPH
                     EVSMREAVAWSAFYVALPVTFGVWVWQAHGPQTGLEYYTGYLVEKSLSVDNLFIFILI
                     LGAFAVPRALQQRVLLIGIAGALVMRAVFIALGAALITRFAWMFLIFGAILLVTAWGV
                     LRDARHGGHAVDVDELRIVKLARRLYPVTDDYHGTRMSIVRAGQRALTPLALVVLAIL
                     GTDLVFAIDSVPAVYGITGDPYLVFVTNAFALLGLRALYFVLEGALSRLRHLGYGLAA
                     ILAFIGVKLVLHWAHGVWPGVPTIPTLASLGVIVAALGLTTLTSVLANRREVSAAS"
     gene            complement(4484..>4707)
                     /locus_tag="PKU75_15195"
     CDS             complement(4484..>4707)
                     /locus_tag="PKU75_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPF82271.1"
                     /translation="FVLQEATLPFESGDEAAAMIREGGFGEGLADQPHMVWFTEQVIL
                     EPGYSFAREFEPGLDLVLDGIEARFTTPR"
BASE COUNT          602 a         1787 c         1594 g          724 t
ORIGIN      
        1 tggcgtcgga gtgcccgatg gcgacgagca ccccggcctc gacgaaccgc cgcaccgcct
       61 ccgaggcacc cggcagctcc ggcgcgaggg tcaccatggc gacggggccg gcggcgatca
      121 gtcggtcgac gtcctccggg gaaggcgccc gcagcagccc ggggtcgtgt gcccccttgt
      181 agcgctccgc cagccagggc ccctcgaggt gcaccccgag cagatcgtcg caggcgacga
      241 gcggcgccag cgcccggacc tggtcgacga gccggtcgac ggtgtcggtt accaggctcg
      301 ccatcgtcga cgtcgcgccg tgggccaggt gggcggcccg ggcgatgccc gcggcctcga
      361 cgccgtcggt gtaggccgcg ccaccgcccc cgtggctgtg gacgtcgacg aacccgggcg
      421 cgagggtggc cgcacccagg tccgtggcca cccactcgtc ccgacccgcc gagcccgtcg
      481 gcccgggggg cagcccgtcg ccgacctcgg tgatgcggtc gccctcgacc cgcacccacc
      541 ccggcgacag cacggcatcg ggcgtgagaa ccgttgcggc cgtgaggacc tgacccccgc
      601 ccccggcggt cgtcgagtcg gcggtcacgt gggcgcggcc agatctgcgg tgatgatggc
      661 gatctccagg gctcgtcggt tccgtccttc ggtggggtga gcgtatgccg cgtcgctcgg
      721 ggtgcgctgg aaacccgcca ccccggccag ccccgccggg gttggggcca agacgtgtcc
      781 agcggccaga ctgccgggat gctggtggcc gagctggagc ggatcatccc gatcatcgtt
      841 gtcctcctcg ccgccgccgt gctcgccgag ctcagtgacc gcgcaggggt gttcgacgtc
      901 gtcgggcact ggctcgcgcg acatgcccgc caccggctcc tcgttctctg gctgctcttc
      961 agtgcgtatg ccgtgatctg caccgtcttc ctctcgctcg acaccaccgc tgtcctgctc
     1021 accccggtcg ccctggcgat cgccaagcag gtgggcgtgc cggcgcggcc gttcgccctg
     1081 acgacgctct ggatcgccaa caccggctcg atgctgctgc cggtctccaa cctcacgaac
     1141 ctgttggcgc agagcaagtt ccgggcgctc ggggtcgacc acacggcata cctccgtctg
     1201 gcgctcgtgc ccggactggt gtgcatcgtc gtcacgctcg tcgtgatcct cgcgctgcac
     1261 ccgcgggtgc tgacgacgca atacacggtc gacccgccgg ccgagccgca cgaccaggtc
     1321 ctgctgcgcg tcgggctggt cacctgccta ctcgtgggac ccgccttcgc cgccgggctg
     1381 gcgccctggg ccgtcgccgg cgtggccgcg ggggtgctgc tgctcgtcac ctggctgcgc
     1441 gccccgcacc tgctccgcgg cctggccgtg ccgtgggtga tggccgccgg gttcgcggtg
     1501 ctggcgctcc tcgtcggctg ggcccagcac gcggggcacc tggcctggct cggccagctc
     1561 gtcggccagg gcaccggcct gaccgacctg ctgcgcgtca ccgggaccgc cgcgctgacc
     1621 tcgaacctcg tcaacaacct ccccgcctac ctggcggtcg agcccttcgc cgccgaccac
     1681 ccggcgcggc tcatggctgc tctcgtcggc gccaatgccg ggccgctcgt cacgccctgg
     1741 gcctcgctgg ccacgctgct ctggctgcag cggtgccggg ccggtgggct gcggtggcgg
     1801 ctcggtcggc tcgcgctcgc cgggctgggg tgtgctctcg cctcgaccgt cctggccgtg
     1861 ctggcgctgg ccctcgtcac cggctgagcg cccggcgcgg gcgtcatgat ggagctcatg
     1921 gcggtcctgt ccttcagcat gaaccagtcc ctcgacggca atgtcgccca cgacgccttc
     1981 gcgcccgggc cgctgatgtt cgcgcacttc atcgaccagg tggccgggct gtcggggagc
     2041 atctacggcc gcacgatgta tgaggtcatg cgctactggg acgaggacca gcccgactgg
     2101 gtcgatgcgg agaaggcgta tgccgaggtc tggcgccgcc agcccaagtg ggtcgtctcg
     2161 cgcaccctgg actcggtggg cccgaacgcg accctggtgc gcggggacgt cgcgagcgag
     2221 gtcgcccgga tcagggccga gcacgacggg cggatcgagg tggccggccc cactctcgcg
     2281 gccggcctgg gcgcgctcgg tctcatcgac gagtacgtca tctacctgca cccggtcgtc
     2341 gccggtccgg ggcccacgtt cttcggcgac gaccggcccc gtctggagct cgtcgcagcc
     2401 gagccactcg ccgaaggcgt cgtcaagctc acctatgcac cggccggaaa cgaaccggca
     2461 ctcccgtgac gccaccggca ccgggagagg tcgcggggag cctggcgggc gagcgcgtgg
     2521 tgggcaggac tgcggccgcc cggttccacc ccggctggtg accccacccg tagctgagcg
     2581 cgtcgtcgtc gatgcggcgc ggcattcggc catcatcgcc gatccgccgg cgccctcttc
     2641 ccccacggct cgccgcgagg ggcaaggatg agggcatgac ctccttcctg atcccgcgac
     2701 ccacccaggg cgacgtcgtg gagctcatcc tcgacgacca tcgcgccttc gaggacctcc
     2761 tgcgcgagtg ccgccgctcc gaccgtgacc gggccacggc ccgagccgcg ctggccgagc
     2821 tcctcgtcgc ccacgccgag gccgaggaga gcgaggtcta tccgacgctg cggaccaagc
     2881 gggccatctc cgcccacaag gaggagcacg gcgaggagga gcacgccgag atcgtcgagg
     2941 ccctcctggc cttcctcgat gccaagggca cgtcgacgaa gaagtacgac gaagcgctcg
     3001 aggagctgtc aaaggtcgtc tcccaccaca actgtgagga ggagctcgac atcctcaacc
     3061 ccgcgcggga cgacgtctcc gtcgcagtgc ggcacgagct cggcgtgaag tgggcgcgca
     3121 agcgcaacgc gctgctcaag gccggttgcg cctcccgcac ccaggtggcg gccctcctca
     3181 aggaggcggt caaggacaag accctgccgc cggagcccgt gcgcgagcag atggacgcga
     3241 tcaaggagcg ggccaagaag aaggccgacg cgctcggtga ggaagccacc tcctgaccac
     3301 tcgggcggct ggcgggtgac gccggcggca ggttgacact cccgcgcgcg gtcacctatt
     3361 tttgacgccc acgaggggag tacttcccaa gtcgtgtccg gtcagttcgc gaggcccatg
     3421 cctcgcccgg cgcgcgcccg acccgggtga agcagacctc gcaccgctac cgcgtgctgc
     3481 gaggacgaca tgagctccct ggtgctttac cctgcaaccc cccgcgaggt cggctcgacc
     3541 gccaccatcg ccgaccccac tctgtgggtc ctcaccgtgg cgggggtcct cgccctgctg
     3601 gccctcgact tcctcatcac gcgccgaccc cacgaggtgt cgatgcgcga ggccgtggcc
     3661 tggtcggcct tctatgtcgc gctccccgtc accttcggcg tctgggtctg gcaggcccac
     3721 ggcccgcaga ccgggctgga gtactacacc ggctacctcg tcgagaagtc cctctcggtc
     3781 gacaacctct tcatcttcat cctcatcctg ggcgccttcg cggtcccgcg agcgctccag
     3841 cagagggtgc tcctcatcgg catcgcgggc gccctcgtca tgcgggcggt cttcatcgcc
     3901 ctcggggccg ccctcatcac gcgcttcgcc tggatgttcc tcatcttcgg cgcgatcctg
     3961 ctcgtcaccg cctggggcgt cctgcgcgat gcccgtcacg gtggccacgc cgtcgacgtc
     4021 gacgagctgc gcatcgtcaa gctcgcccgc cgcctctacc cggtgaccga cgactaccac
     4081 ggcacgcgga tgagcatcgt ccgcgcgggt cagcgcgcac tcacccccct tgcgctggtg
     4141 gtcctggcga tcctcgggac cgacctcgtc ttcgccatcg actccgtgcc ggcggtctac
     4201 gggatcaccg gcgaccccta cctcgtcttc gtgaccaacg ccttcgcgct tctcggcctg
     4261 cgagccctct acttcgtcct cgagggggcg ctgagcaggc tgcgccacct cggttacgga
     4321 ctggctgcga tcctcgcctt catcggcgtc aagctcgtcc tccactgggc gcacggggtg
     4381 tggcccggcg tgccgacgat cccgaccctg gcctcgctcg gcgtcatcgt cgccgccctc
     4441 gggctgacca cgctgaccag cgtcctggcc aaccggcggg aggtcagcgc ggcgtcgtga
     4501 accgggcctc gatgccgtcg aggaccaggt cgagccccgg ctcgaactcc ctcgcgaacg
     4561 agtagccggg ctcaaggatg acctgctcgg tgaaccacac catgtgcggc tggtcggcga
     4621 gcccctcgcc gaagccgccc tcgcggatca tcgcggccgc ctcgtcgccg ctctcgaagg
     4681 gcagcgtcgc ctcctggagg acgaagc
//
LOCUS       DAOPJG010000227         4686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_19934_length_4686_cov_4.561002, whole genome shotgun sequence.
ACCESSION   DAOPJG010000227 DAOPJG010000000
VERSION     DAOPJG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19934_length_4686_cov_4.561002"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..203)
                     /locus_tag="PKU75_15200"
     CDS             complement(51..203)
                     /locus_tag="PKU75_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82272.1"
                     /translation="MTPHASDPETRLEHNDTKHLLDDIEALRTHLGIDTWIVNGVSWG
                     STLALA"
     gene            complement(233..415)
                     /locus_tag="PKU75_15205"
     CDS             complement(233..415)
                     /locus_tag="PKU75_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82273.1"
                     /translation="MTAQPEPRRAGLLPVAWGDEIYWEEWGSASGLPALYLHGGPGGS
                     LGTSAYRHRFDLDRSA"
     gene            complement(600..1253)
                     /locus_tag="PKU75_15210"
     CDS             complement(600..1253)
                     /locus_tag="PKU75_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF82274.1"
                     /translation="MTIRVIVADDQELVRTGLQVLIDRADDLTVVAEADNGRTALAAC
                     RDHRPDVVLMDIRMPVMDGIEATRAVAADPALAGTRVIVLTTFDTDENIFAALEAGAA
                     GFLTKDAEPDDLRAAVRTVAGGQALLSPSVTTRVVDRALTGRTADPGAARIVADLTER
                     EREVLVEIARGATNDELAATLFISPATARTYVSRLLTKLDARDRVALVLLAHRAGLA"
     gene            complement(1250..2428)
                     /locus_tag="PKU75_15215"
     CDS             complement(1250..2428)
                     /locus_tag="PKU75_15215"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPF82275.1"
                     /translation="MPTPAPSRDDTTVILDRVVPVIALVLTVTALVSAERRVGAAGDV
                     RDWAWLLPAFLGGLLLLRRRAPVAVLVASVAAVIGYYATGQPAIGLELPLAAAFFSTA
                     ERGRTRATIGMALLVLVIAYGYRVGIAKVEFAALLGLDIMTSIVVLSLAIAAGDGVRS
                     RAAERTANAERYQLQTELVEENLRAETDAERRALARDVHDVLGHAMVVVSTQANVALE
                     SIADQETTSAALRNIKHTAQQSLRDIRGSVALLSGGQQSERRPVGTLADLGELAARFE
                     GTGLTIEIRSEGDLATLPAAVSSTAYRVIQEAVTNSVRHGAARRVDITLGRTQDTLQV
                     TVRDDGRGAGAFTMGHGLRGMQDRVAMLGGSVHAGPGPENGFHVQARIPLAPARREAP
                     "
     gene            2560..>4686
                     /locus_tag="PKU75_15220"
     CDS             2560..>4686
                     /locus_tag="PKU75_15220"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPF82276.1"
                     /translation="MRKLTRLCIHHRLLTIAAWVLLLVGLTTAAQLAPGPTGNDYSVP
                     GAPSAKASAFLADHGLDDGEATTAQIVLHDSAGLAAHQGAITSYARHLSAEHPTWEIR
                     PGPGEAGALSEISADGTIGYLDVTVAEDTLGDAAKESAASAITDAAPKAPGLSVEYAG
                     EIFPEPSSGPGESLGMLAAVVILLIAFGSLLAMALPLVIAAFGAGTGVMALGLAGHLI
                     ELPGAAGALAMMLAIGVGIDYSLLVVTRYRERLAAGRAPREAVMESMETAGHSVLVAG
                     ATVVVAILGLLVMNMPMVNGIAVGAALAIAMTMLAALTLLPALLVTVGKRIDKFGLPH
                     RGRARDGHGGAFTRWANWVQRHPWGATIASLVALVALALPALDMRLGFADDGSSPTST
                     TQRRAFDLLSEGFGAGTHGPLVLAIDTAAVTGPAEQDALGAMGSRLEQVPGVDRVEGP
                     FPSADGEAAAAIVVPATGPGDVATEDLVHRLRDDVLPGAALPAEVTGTTAAAVDFADY
                     TRERLPVFVAVVLGLAFLLLARTYRSLVVPAKAVLMNLLSIGAAFGVVVAVVQWGWGA
                     ELLGFENPGPVTAWVPMMLVAVVFGLSMDYEVFLLSRIKEYVDRGHDNTEAVTLGLAR
                     TGRVITAAAATMVCVFGGFVLGHDLELKIFGLGLAAAVLIDATIVRMLLVPAAMALMG
                     SANWWAPRWMRRREPVPAVTSDGTEPE"
BASE COUNT          656 a         1633 c         1661 g          736 t
ORIGIN      
        1 caggcccacg cccgaccccc ggggaggcgc gccttttgtt cagcagtctc ctaggccagc
       61 gccagagtgc ttccccagga gaccccgttc acgatccagg tgtcgatgcc gaggtgggtc
      121 cgcagggcct cgatgtcatc gaggaggtgt ttcgtgtcgt tgtgttcgag gcgggtctcg
      181 gggtcgctcg cgtgtggcgt caacctcccg cagccacgtt gctcgaaccc gatcacgcgg
      241 accggtcgag gtcgaagcgg tgtcgatagg cgctcgtccc gagcgagccg cctggcccgc
      301 catgcagata gagtgcgggc aatccggacg cggatcccca ttcctcccag tagatttcgt
      361 ccccccacgc caccgggagc agccccgcgc gccgcggttc gggttgcgca gtcatgggcg
      421 acaagccaac acgagtaggt cggatgccgc aagcaatgcg cacggcgttc agcgccccga
      481 gccgacgtgg tggctgacag actcggtgct tcggccgacg gcgcacgacg aaggagcgat
      541 acccgacgat gagtgaccga acgcaggtga ttgacgcctt ccagtcgcag gcgtgagggt
      601 caggcgagcc cggcccggtg cgccagcagc accagcgcga cccggtcccg ggcgtccagc
      661 ttggtcagga ggcgactgac ataggtgcgc gcggtcgccg gcgagatgaa gagggtcgct
      721 gccaactcgt cattggtcgc accccgggcg atctcgacga gcacctcgcg ctcccgttcg
      781 gtcaggtcgg cgacgatccg agcggcgccc gggtctgcgg tgcgtccggt cagggcacgg
      841 tcgacgactc gcgttgtgac agagggggat agcagcgcct ggccgccggc caccgtccgc
      901 acggcggcgc gcaggtcgtc cggctccgcg tccttggtca ggaagcccgc ggccccggcc
      961 tccagggccg cgaagatgtt ctcgtcggtg tcgaaggtgg tcaggacgat gacacgggtc
     1021 ccggccaggg ccgggtcggc cgcaacggct cgggtcgcct cgatgccatc catgaccggc
     1081 atccggatgt ccatcaggac gacgtcggga cggtggtcgc ggcacgcggc gagggcggtg
     1141 cgtccgttgt ccgcctcggc gacgacggtg aggtcgtcgg cacggtcgat cagcacctgc
     1201 aggccggtgc ggacgagttc ctgatcgtcg gcgacgatca cgcggattgt cacggcgcct
     1261 cccgacgggc tggggcgagg gggatcctgg cttgcacgtg gaacccgttc tcgggtcccg
     1321 gcccggcgtg cacggagccc ccgagcatgg cgacccggtc ctgcatccca cgcagcccgt
     1381 gtcccatcgt gaacgcaccg gctccgcgtc cgtcgtcgcg gaccgtgacc tgcagggtgt
     1441 cctgtgtccg acccagcgtg atgtcgaccc ggcgggccgc accgtgccgg acggagttgg
     1501 tgacggcctc ttggatcacc cggtatgccg tgctgctcac cgccgcgggt agcgtcgcga
     1561 ggtcgccttc cgacctgatc tcgatggtca gtccggtgcc ttcgaaccgg gccgccaact
     1621 cgcccaagtc ggccagcgtc ccaaccgggc ggcgctccga ctgctgtccg ccggacagca
     1681 gggccactga tccgcggatg tcccgaagtg attgttgcgc agtatgtttg atgttcctga
     1741 gtgccgcgct ggtggtctcc tgatccgcga tcgactccaa ggcgacattg gcctgcgtgg
     1801 agacgacgac catggcgtgg cccaggacgt cgtggacgtc gcgggccagt gcccggcgtt
     1861 cggcgtcggt ctcggcgcgc aggttctcct cgaccagttc ggtctgcagc tggtaccgct
     1921 cggcgttcgc ggtccgttcg gccgcccggg aacgcacgcc gtcgccggcg gcgatcgcga
     1981 gcgagagcac gacgatggag gtcatgatgt ccagcccgag caaggcggcg aactccacct
     2041 tggcgatgcc gacccggtag ccgtaggcga tgaccaggac gaggagcgcc atgccgatgg
     2101 tggctcgggt ccggccgcgc tcggccgtgg agaagaaggc cgccgcgagc ggtagttcca
     2161 ggccgatcgc cggctggcct gtggcgtagt agccgatgac ggctgcgacc gatgcgacca
     2221 gaaccgccac gggggcccgc cgccgaagca gcaacagacc cccgaggaac gcgggaagga
     2281 gccacgccca gtcccgaacg tcgcccgccg cacccacccg gcgctcggca ctgacgagcg
     2341 cggtcacggt cagcacgagg gcgatcacgg gcaccacgcg gtcgaggatc accgtggtgt
     2401 cgtcgcggga cggggcgggc gtcggcaccc tccgacgcta ccgggcccgg cgggactcgg
     2461 ggagtcgacc ggtgtcgaca acgaagtgtc cacacccggc tgattccgtg tcgtcgtgga
     2521 gcggatggca agcagtctcg ggtcccggag atttgaggca tgcgaaaact caccagactc
     2581 tgtatccacc accgtctcct cacgatcgcc gcctgggtcc tcctgctcgt cgggctgacc
     2641 acggcagcac aactggcacc cggccccacc ggcaacgact acagcgtccc cggcgccccc
     2701 tccgcgaagg cctcggcctt cctcgccgat cacggcctgg acgacgggga ggcgaccacc
     2761 gcgcagatcg tcctgcacga ctccgccggc ctcgccgctc accagggcgc catcacttca
     2821 tacgcgcggc atttgagcgc ggagcatccg acctgggaga tcaggcccgg acccggcgag
     2881 gccggggccc tgtcggagat ctccgcggac ggcaccatcg gctacctcga tgtcacggtt
     2941 gccgaagaca ccctcggcga cgcggcgaag gagagcgcgg cgagcgccat caccgacgcc
     3001 gcaccgaagg caccggggct gagcgtcgag tatgccggcg agatcttccc cgagcccagc
     3061 agcgggccag gggaatcgct cggcatgctc gccgccgtgg tcatcttgct catcgccttc
     3121 gggtcgctgt tggcgatggc gttgcccctg gtcatagcgg cgttcggggc cgggaccggt
     3181 gtcatggcgc tcggtctcgc cggccacctc atcgagttgc ccggtgccgc cggtgcgctg
     3241 gcgatgatgc tggccatcgg ggtcggcatc gactactcgc tcctggtcgt cacgcggtac
     3301 cgggaacggc tggccgcggg ccgcgcgcct cgggaagcgg tcatggagtc gatggagacg
     3361 gcggggcact ccgtgctcgt cgccggagcg acggtcgtcg tcgccatcct cggcctgctc
     3421 gtgatgaaca tgccgatggt gaacgggatc gctgtcgggg ccgcactcgc catcgccatg
     3481 accatgctcg ccgccctcac gctcttgccg gcgctgctcg tcacggtcgg gaagcggatc
     3541 gacaagttcg gcctgccgca tcgcggccgc gcgcgcgacg gccatggcgg cgcgttcacc
     3601 cggtgggcga actgggtgca gcgtcacccc tggggcgcca cgatcgccag tctcgtcgcg
     3661 ctggtcgctc tggccttgcc cgcgctggac atgcggctgg gcttcgcgga cgacggcagc
     3721 tcgcccacgt cgacgaccca gcgccgcgcg ttcgacctgc tgagcgaggg gttcggcgcc
     3781 ggcacgcacg gaccgctcgt gctggcgatc gacacggccg cggtcaccgg ccccgccgag
     3841 caggatgcgt tgggcgccat gggttcacgt ctcgaacagg tgcccggtgt cgatcgcgtc
     3901 gaaggtccct tcccgagtgc ggacggcgag gcggcggccg cgatcgtcgt gccggccacc
     3961 ggccccggcg acgtggcgac ggaggatctc gtccaccgtt tgcgcgatga cgtcctcccg
     4021 ggagcggccc ttccggccga ggtgaccgga accactgctg cggctgtcga cttcgccgac
     4081 tacacgcgag agcggctgcc ggtattcgtg gccgtcgtcc tcggcctggc cttcctgttg
     4141 ctggcccgga cctatcgcag cctcgtcgtc ccggccaagg ccgtgctgat gaacctgctg
     4201 tccatcggcg ccgccttcgg cgtcgtcgtc gccgtcgtcc aatggggctg gggcgcagag
     4261 cttctcggct tcgagaaccc cggccccgtg acggcctggg tgccgatgat gctggtcgcc
     4321 gtggtcttcg ggctctcgat ggactacgag gtcttcctcc tgtcccgcat caaggagtac
     4381 gtggaccgcg gtcacgacaa caccgaagcg gtcaccctcg ggctggcgcg caccggccgc
     4441 gtcatcaccg ccgccgcggc gaccatggtc tgcgtcttcg gcgggttcgt cctcggacac
     4501 gatctggagc tgaagatctt cggcctcggg ctggccgccg ccgtgctcat cgatgcgacg
     4561 atcgtgcgga tgctgctggt tccggcggcg atggcgctga tgggttcggc gaactggtgg
     4621 gctccacggt ggatgcgccg ccgcgagccg gtgcccgctg tcacctccga tggcaccgag
     4681 ccggag
//
LOCUS       DAOPJG010000228         4666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_20065_length_4666_cov_4.882889, whole genome shotgun sequence.
ACCESSION   DAOPJG010000228 DAOPJG010000000
VERSION     DAOPJG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20065_length_4666_cov_4.882889"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKU75_15225"
     CDS             <1..325
                     /locus_tag="PKU75_15225"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPF82277.1"
                     /translation="KATETLLNENRSPARKVGQIDNRGSHAYLATYWAQELARQTEDE
                     KLAKTFTSVAEALTAQESAINDELIGVQGHPADIGGYFRPDAEKTSGVMRPSATLNEV
                     IDSIG"
     gene            complement(401..1186)
                     /locus_tag="PKU75_15230"
     CDS             complement(401..1186)
                     /locus_tag="PKU75_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPF82278.1"
                     /translation="MTSRAQYAGLTALLVATLVLVAFALRTPAAPTGSGSSVARPTPT
                     SASPSPSVSEASPSEPVAIGEGAKVAFVGDSFAAGAGASGEQARWTTVLSAAHKWSED
                     NLGRAGTGYLRAGRTENCTPQNCPAYPGLVGQIVADRPALVVITGGGNDLGQDLTQLG
                     AAVTQTVKGIKDGVPGVRVVVTNPWWDMRPYDQRLATYTQAIQKAATDAGAVWVDTAQ
                     PVMGKPDLMTADGMQPNDRGHEAIATSVGRGLEQAGLIAKAAQ"
     gene            complement(1738..2903)
                     /locus_tag="PKU75_15235"
                     /pseudo
     CDS             complement(1738..2903)
                     /locus_tag="PKU75_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            2977..>4666
                     /locus_tag="PKU75_15240"
     CDS             2977..>4666
                     /locus_tag="PKU75_15240"
                     /inference="COORDINATES: protein motif:HMM:NF016903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HPF82279.1"
                     /translation="MPESPSVTRVVCLSLHPTDGAINRTVLPMLDALREVADRLVVVS
                     VGPLDPAVRAELVTSADRVEEPPDGARLFAAVAEESAGGSVEVLLVDDSCVGPFGSFA
                     DVFTRMADTELDAWSLTSRLDPHGGEHLDAYWILLRPSALATPTVREFLERSNRPSSE
                     DPGAPGQPDRRAGPEDHPGQLVDAIHHSPLRRAAAWPQRDYHTTEPALESADLLLDDG
                     APVVPLDAFGRLPMALEERAVIGRRLLEAVERTNYPMEHLWATLARTAEPRHVHTDLG
                     LVEILSGEEADAPSPAPRVAVLAHVFYVDLVPEMMLSVSNIPVPYALHVTTDTAEKRT
                     TILDLLAGHDFDHVDVRIVESNRGRDLAALLITQRDLLLGGDYDLICRVHSKKSPQDS
                     PNVGRLFREHLYDNLLGSRGYVARLLRLFTEHPTLGMVFPPVIHIGYPTLGHAWFANR
                     EPAQELAERLGIGTRFDAHTPLAAYGSMFWARPEALRAIAQAPWRHEDFPADGEYRDG
                     SLTHVIERLLAYAAIDAGYHVRSAMDTTWAAINYAFLEYKLAAIGAFLPGTTRDQ"
BASE COUNT          692 a         1651 c         1596 g          727 t
ORIGIN      
        1 caaggcaacc gagaccctcc tcaacgagaa caggtcaccc gcccgcaagg tcggccagat
       61 cgacaaccgc ggcagccacg cctacctcgc gacctactgg gcccaggagc tggcccggca
      121 gaccgaggac gagaagctgg ccaagacctt cacctcggtc gccgaggccc tcactgcgca
      181 ggagtcggcg atcaacgacg agctcatcgg cgtccagggg catccggccg acatcggcgg
      241 ctacttccgc cccgatgccg agaagacctc cggggtcatg cgcccgtcgg ccaccctcaa
      301 cgaggtcatc gacagcatcg gctgagacaa cccagtgcgc gaatgccgcg tgcctcccga
      361 tcgcgagaac gcggcattcg tcgtgatggg cccgaatggg ctactgcgcg gccttggcga
      421 tgaggccggc ctgctccagc ccgcggccga ccgacgtggc gatcgcctcg tgccccctgt
      481 cgttgggctg catcccgtcg gcggtcatca gatccggctt gcccatgacc ggctgggcgg
      541 tgtcgaccca gacggcaccg gcgtcggtcg cggccttctg gatggcctgg gtgtaggtcg
      601 caagccgctg gtcgtagggg cgcatgtccc accacgggtt cgtcacgacc acccggacgc
      661 cggggacgcc gtccttgatg cccttgaccg tctgggtgac ggccgcaccg agctgagtga
      721 ggtcctgacc gagatcgttg ccgcccccgg tgatgactac gagggcgggc ctgtccgcga
      781 cgatctgccc gaccaggccg gggtaggccg ggcagttctg cggggtgcag ttctccgtgc
      841 gcccggcacg caggtatccg gtccccgccc ggccgagatt gtcctcgctc cacttgtgcg
      901 cggcggagag gaccgtcgtc cagcgcgcct gctccccgct cgccccggca ccggcggcga
      961 acgagtcacc gacgaaggcg accttggcgc cctcaccgat cgccaccggc tcgctgggcg
     1021 aggcctcgct cacactgggg ctcgggctgg cgctcgtcgg ggtcggccgg gccaccgacg
     1081 agccggagcc cgtcggggcg gcgggcgtcc gcagggcgaa ggcgacgagg acaagggtgg
     1141 cgacgagcag ggcggtgagg cctgcgtact gggcacggga agtcatggtg ggcacatcct
     1201 ctcacccaca ccactcccgc accgaacgat acaagtcccg ctcagagtgc catcttgaag
     1261 ccctcgtggc tcgccgcgta cgcgagccgc gtgtagaagc ggtgcgaatc gacccgctgc
     1321 ttgtcgctcg tcagctgcag gacgacgcag ccgcgccggc gggcctcctc ctcggcccac
     1381 gtgagcagtg gctccccgag ccggtgcccg cggctcggtc gccgccgagg tcgcccacca
     1441 cggggatgtc gcgatctgcc gccccatcgc gccgcgcgag aaggcccacc aggaggtggg
     1501 tgagcttgtg cgccgtcgcg gcgggcggtt cgccctggtt cacgtcgcca cgccgctcga
     1561 ggagtgcggg cggcgtgacg ccaaggggct ctaccctggg gagctcgccg ggatcggccc
     1621 tcggctaact caccgaggtc gactgggtca tctgactcgc gctgactcga ttcgggctga
     1681 cggagtcggt gcgtgtgaat acgccgagag tcaccggtga gttcacacac cccgagatca
     1741 gtcgcgcccg cagaggtcgc gcgtgtagac cttgtcggcg acgtcgtcga gggccgaggt
     1801 gcgccggttg gcgatgatca ggtcggcacg ctccttgaac tcggcgaggt cggcgacgac
     1861 ctcggagttg gagaagcggt cctcgtgcag ggtcggctcg tggacgatga cctcgacacc
     1921 cttggccttg agccgcttca tgaccccctg gaccgacgag gtcctgaagt tgtccgatcc
     1981 cgccttcatg atcagccggt ggatgccgac gacccgcggc tcacgcgcga ggatgtcggc
     2041 ggcgatgaag tccttgcggg tcgtgttgct ctcgacgatg gcgcggatga ggttttgcgg
     2101 cacttggtcg tagttggcga ggagctgctt ggtgtccttg ggcaggcagt agccgccgta
     2161 gccgaaggac gggttgttgt agtgcgtgcc gatccgcggg tcgaggccga cgcccccgat
     2221 gatctggcgg ggggtcgagg tcgtggctga tcgcgtagga gtcgagccgt tgaagaaggc
     2281 cacccgcatg ggcaggtaag tgttggcgaa gagcttgatc gcctcggcct ccgtggagcc
     2341 ggtcagcagg acgggcacgt ccggggccag ggagccctcg acgagcagcc gcgcgaaggt
     2401 ctccgcctcc gggccgacgc caccgacgaa gatccggctc gggtggaggt tgtcgtgcag
     2461 ggcccgccct cccgcaggaa ctccggggag aagaggatgc gctggccggg gaattgctcg
     2521 cgcaggctgc gggtgtagcc gacggggatc gtcgacttga tgatgaccgt cgactcgggg
     2581 gccagccggg ccacgtcggc gacacggact caaccgcgct ggggtcgaag aatttgcggt
     2641 ccgggtcgta cttcctgggg gtcgccacga cgacgaagtc cgccggggca taggcgacct
     2701 gtggggtcgg tgatggcgct cagcctcagc tcgcgcgtcg cgaggtgctc ggccaactcc
     2761 gggtcgtcga tcggggaccg gcgttcggtg accagccgga cccgctgctc gtcgatgtcg
     2821 agggccacca cctcgtgctg ttgggccagg acggcggcgt tggacagtcc gacatcgccc
     2881 aggcccgcga cggcgatctt cacaaggcaa cgctagggca tcgggcaccg cgacatcgtt
     2941 ccccgcctgc cgagccgatg aacgtagagt tgggacatgc ccgagtcccc ttccgtcacc
     3001 cgggtcgtgt gtctgtccct gcaccccacc gacggcgcca tcaaccgcac cgtcctcccg
     3061 atgctcgacg ctttgcggga ggtcgccgac cggctcgtgg tcgtcagcgt ggggccgctc
     3121 gaccccgcgg tccgcgcgga actggtgacg tcggcagacc gtgtcgagga gccgcccgat
     3181 ggcgctcggc tcttcgccgc cgtggccgag gagtctgcgg gtgggtcggt ggaggtgctg
     3241 ctcgtcgacg actcatgcgt cggtcccttc gggtccttcg cggacgtctt cacgaggatg
     3301 gccgacaccg aactcgacgc ctggtccctc acgtcgcgcc tcgaccccca cggtggcgag
     3361 caccttgacg cctactggat cctgcttcgt cccagcgccc tggcgacgcc gaccgttcgc
     3421 gagttcctgg agcgctcgaa ccgaccgagc agtgaggacc cgggcgcgcc ggggcagccc
     3481 gaccggcggg cggggcctga agaccacccc ggccagctcg tcgacgccat ccaccattcc
     3541 cccctgcgcc gtgccgccgc gtggccgcaa cgcgactacc acaccacgga gcctgctctc
     3601 gagagcgcgg acctgctcct tgacgacggg gcccccgtgg tgccactcga cgccttcggg
     3661 cggctaccca tggcactgga ggagcgagca gtcatcggcc gccgcctgct cgaggcggtg
     3721 gagaggacca actacccgat ggagcacctc tgggcgacgc tcgcccggac ggcggagccc
     3781 cggcatgtcc acaccgacct cggactggtc gagatcctgt ccggggagga ggccgacgca
     3841 ccctcacctg cgccccgcgt ggccgtcctg gcccacgtgt tctatgtcga cctcgtcccc
     3901 gaaatgatgc tctccgtcag caacatcccg gtgccctacg ccctgcacgt gacgaccgac
     3961 accgcagaga agcggaccac gatcctcgac ctgctggccg gtcacgactt cgaccacgtc
     4021 gacgtccgca tcgtcgaaag caaccggggc cgcgacctcg ccgccctcct catcacccag
     4081 cgcgacctgc tcctcggcgg cgactacgac ctcatctgcc gcgtgcactc caagaagtcg
     4141 ccgcaggatt cccccaatgt gggccgcctt ttccgcgagc acctttacga caacctcttg
     4201 gggtcgcggg gctacgttgc ccgcctgctg cgcctgttca ccgagcaccc caccttgggc
     4261 atggtcttcc cgccggtcat ccacatcggc taccccaccc ttggacacgc ttggttcgcc
     4321 aaccgtgagc ccgcccagga gctggccgag cgcctcggca tcggcacccg tttcgatgcc
     4381 cacaccccgc tggcggccta cgggtcgatg ttctgggcgc gcccggaggc cctgcgagcc
     4441 atcgcgcagg caccgtggcg ccacgaggac ttccccgccg atggcgagta ccgcgacggc
     4501 agcctgaccc acgtcatcga gcgcctgctc gcgtatgccg cgatcgacgc gggctatcac
     4561 gttcgctcgg cgatggacac gacatgggct gcgatcaact atgccttcct cgagtacaag
     4621 ctggcggcca tcggtgcctt cctgccgggg accacacgtg accagg
//
LOCUS       DAOPJG010000229         4598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_20495_length_4598_cov_8.188422, whole genome shotgun sequence.
ACCESSION   DAOPJG010000229 DAOPJG010000000
VERSION     DAOPJG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20495_length_4598_cov_8.188422"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKU75_15245"
     CDS             complement(<1..518)
                     /locus_tag="PKU75_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HPF82280.1"
                     /translation="MTHTDAVNAGRAGANRRDGNEISAEQATALEAALGKALAAKGLR
                     TSSSGRVVSASGAAAFAATTVNVYFHVITDGTKGNLSSTMISKQMTVLNNAYAGTGFS
                     FTLAGTDYTNNASWYNLSNGSTAERAMKTALRQGTMADLNIYTANLGGGLLGWATFPK
                     SSYDAMDGVVLL"
     gene            812..1102
                     /locus_tag="PKU75_15250"
     CDS             812..1102
                     /locus_tag="PKU75_15250"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HPF82281.1"
                     /translation="MARRYDEPVDVQSRDEVMSAFIWRDRLYAVRGVLDRWTERRSWW
                     QDPVGAPRTGERTVWRVEASAGRCAGTGIYDIGRDTDAAAHEPAWTLLRVQD"
     gene            1114..1566
                     /locus_tag="PKU75_15255"
     CDS             1114..1566
                     /locus_tag="PKU75_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV_6107 family HEPN domain-containing protein"
                     /protein_id="HPF82282.1"
                     /translation="MTVRVIAKGERVQATRVASALDLLRGSEASLVEAGHAAHVAQRY
                     AGAHLAALRAAAAVLAVRGPRLAGSRGSGGPRNLWELLPAVAPELHEWADFFALSSAR
                     RARPDEAISSREADDLVRSAQEFHDRVRVLLGLTRRSGPLLLSPIAVH"
     gene            complement(1595..2107)
                     /locus_tag="PKU75_15260"
     CDS             complement(1595..2107)
                     /locus_tag="PKU75_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF82283.1"
                     /translation="MSLSEKEFEQLYRELGPRILAFLMRRVHAGDAPDLLAETFLVVW
                     RRRDTVPGGDVRAAWVFGVARRLELAHHRDGMTRPTPVADPPEPAGPAACVPEAASAD
                     ERSRCVQAALATLGDIDRELVLLTVWDGLTPAQAGAVVGLAPETARARLHRARTRMEA
                     QLSALLPAHC"
     gene            2505..>4598
                     /locus_tag="PKU75_15265"
     CDS             2505..>4598
                     /locus_tag="PKU75_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPF82284.1"
                     /translation="MTEFAHLHLASGFSMRYGTAMPEALVERAAQYGQPAVALTDRDG
                     LYGAVRFVQAAAKAGLDAVLGVDLAMARGILDAPPDPRARRPRTPVRGGAIVDPRLPR
                     ITVLASGIGGVHGPHTGISYAEGWAALCRLVTDTHLRGERGNPIGTPEQVASFADSET
                     GGRSGPEARGTARNPARLVVLLGPDSDVGRAVLRKDLAGAEALLRAWGAILPRDGLVV
                     EIVCHGGPDGTPGSRNHAARMLNLADRCGIPAILAAAVRHIDPVDARIVDVLDAARRL
                     VMLDRRHLDRVSEAGHLATTETMHRIAREVAERAGADPSRAGRLLADTLALAHRCAQH
                     PRHDLGIGAVHLPEPHILGIPEGTDPQRVLDERCHAAILTRYPGASESERRAVEARLR
                     EELDVVAALGYPTYFMTVATVVDLIREMGVRVAARGSGAGSLVNYLLGISGVDPIRYD
                     LLMERFCSPLRAELPDIDIDVESARRTDIYEQILDRFGGERVTCVSMMDTYKVRHAIR
                     DVGSVLGMPPAEIDEIAKAFPHIRARDARQAIADMPELRQRGLDSDQLTTLFDLVEGL
                     DGLPRHIALHPCGVILSNGTLLDRTPVEASWLGFPMSQFDKDDVEAVGLLKLDVLGIR
                     MQSAMAHAVAEVARVDGVTVDLDDPAQVPLDDEATYAMIRTTRTLGCFQIESPGQREL
                     IGKLGPERFEDLVIDI"
BASE COUNT          629 a         1587 c         1688 g          694 t
ORIGIN      
        1 tcgaggagca cgacgccgtc catcgcgtca tacgagctct tggggaaggt cgcccagccg
       61 agcaggccgc caccgaggtt ggcggtgtag atgttcaggt cggccatcgt gccctggcgc
      121 agcgcggtct tcatcgcgcg ctccgcggtg gagccgttgc tgaggttgta ccagctggcg
      181 ttgttcgtgt agtccgtgcc ggcgagggtg aagctgaagc cggtcccggc gtaggcattg
      241 ttgagcacgg tcatctgctt ggagatcatc gtcgagctca ggttgccctt ggtgccgtcg
      301 gtgatgacgt ggaagtagac gttgacggtg gtcgcggcga aggcggcggc gcccgatgcg
      361 ctcacgacac ggcccgacga ggaggtacgc aggcccttgg cggccagggc cttgccgagg
      421 gcggcttcga gggccgtggc ctgctcggcg gagatttcgt tgccatcgcg gcggttggcg
      481 ccggcgcggc cggcgttgac ggcgtcggtg tgggtcacgc acgcggcgcc gggggcgtcg
      541 gtgctgagcg ggcgcgcgga ggcggacggg gcggtggctc cgaaggcggc cagggtgagc
      601 aggccggcac ctgcggcgga ccgccgctgc ctacccggga gtagccatca agttcgacga
      661 atcggcgaac ggtcgcgagg ccggggccgg ccggaaccgg ggcttgactc ccgcgctccg
      721 gcggggagta gagtcctcgt catagaacac ctgttcgatg aacgaatgct cgatctccag
      781 aatactcgcc cccgcgtctc caggagtgcc gatggcccgc cgctacgacg aacccgtcga
      841 cgtccagtcc cgggatgagg tgatgtccgc cttcatctgg cgtgaccggc tgtatgccgt
      901 gcgcggagtc ctcgaccgct ggaccgagcg ccgctcatgg tggcaggacc ccgtgggggc
      961 gccccggacc ggagagcgca ccgtatggcg tgtcgaggcc agcgccgggc gctgcgccgg
     1021 cacggggatc tatgacatcg ggcgagacac ggatgccgcc gcccacgaac cggcgtggac
     1081 cctgctgcgg gtccaggact gatggaggaa cccatgactg tccgcgtcat cgccaagggc
     1141 gagcgggtcc aggcgacccg ggtcgcctcg gcgctcgatc tcctgcgcgg atcggaggcg
     1201 agcctggtcg aggccgggca cgccgcgcac gtcgcgcagc ggtatgccgg ggcgcacctc
     1261 gccgccctgc gcgccgccgc tgcggtgctt gccgtccgcg ggccgcgact ggccgggtcg
     1321 cgggggagcg gcgggccgcg caacctgtgg gagttgctcc ccgccgtcgc cccggaactg
     1381 cacgagtggg ccgacttctt cgccctcagc tcagcccgcc gggcgcgccc ggacgaggcc
     1441 atttcatctc gcgaggccga cgacctggtc cgctctgcgc aggagttcca cgaccgggtc
     1501 cgggtgctcc tcggactgac tcggcgttcc ggcccgctgc tgctgagtcc tattgcggtg
     1561 cactgacatc cgggcctttg cgacgagcgg gtggtcagca gtgcgctggc aggagtgcgg
     1621 acagctgggc ctccatgcgg gtccgtgcgc ggtgcaggcg cgcccgtgcc gtctccgggg
     1681 ccaggccgac gacggcgccc gcctgggccg gcgtgaggcc gtcccagacg gtcagcagga
     1741 ccagctctcg gtcgatgtcg ccgagggtgg ccagggcggc ctgcacacat cgtgagcgct
     1801 cgtcggcgct tgcggcctcg ggcacgcagg ccgccggccc ggccggttcc ggcgggtcgg
     1861 cgaccggggt ggggcgcgtc ataccgtcac gatggtgcgc gagctccagc cgtcgcgcca
     1921 cgccgaagac ccacgccgcg cgaacgtccc cgccgggcac ggtgtcgcgc cggcgccaga
     1981 cgaccaggaa ggtctcggcc aacaggtcgg gcgcgtcgcc cgcgtggacc cggcgcatga
     2041 gaaaggccag aatccgtggg ccgagttccc ggtagagctg ctcgaactcc ttctcggaca
     2101 agctcatttc gcgcgaccgg cgagggcgtc cgcggcgatc cgctgcatgt ccggctcggt
     2161 ccacggcatg gcgcggggtc gagggctgtc acggccgccc aggtcagggc ctcttcgacc
     2221 tgcgagagag cgacggcggc ccgtggcgga tcggaggttc gggagacaac ccaggtgtcc
     2281 gcccacgagc agatcgggcg tgctggtcgc tgccgggtcg gcctggcgca gcaggtgggc
     2341 gaggtcggtc atcgtgggcc tccttggccg tcaggtgaat cgtgccgtgc tttcactgag
     2401 tgggtgcgca ggcgttacac caggtgttgc gcgggccggg aagcaaacct aggattagta
     2461 caaatgttct agtcccgttt gtcgacccga aagttcctgt ccgcatgacc gagttcgcgc
     2521 acctgcacct cgcctcgggc ttctcgatgc gttacggcac ggcgatgccc gaggcgctgg
     2581 tggaacgggc cgcgcaatac ggccagccgg cggtcgcgct caccgaccgg gacgggttgt
     2641 atggcgcggt gcgcttcgtc caggccgctg ccaaggccgg tctcgacgcc gtgctcgggg
     2701 tcgacctggc gatggcccgc ggcatcctcg acgccccgcc cgacccgcgt gcccgccggc
     2761 cgcgaacgcc ggtgcgcggc ggcgcgatcg tcgaccctcg gctgccgcga atcacggtgc
     2821 tggccagtgg aatcgggggc gtccacggac cgcatacggg gatttcgtat gccgagggct
     2881 gggccgccct gtgtcgcctg gtcaccgaca cccacctgcg cggcgagcgc ggcaacccca
     2941 tcgggacgcc cgagcaggtg gcgagcttcg ccgacagcga aacgggaggg agaagcggac
     3001 cggaggcaag ggggacggcg cgcaacccgg cccgtctggt cgtgttgctg gggccggact
     3061 ccgacgtggg gcgggcggtg ctgcgcaagg acctcgccgg tgccgaggca ttgttgcggg
     3121 cctggggggc gatcttgcct cgcgacgggt tggtcgtcga gatcgtctgc cacggcggtc
     3181 ccgacggcac gccgggctcg cgcaatcacg ccgcccggat gctgaatctc gctgaccggt
     3241 gcgggattcc ggcgattctc gccgcggcgg tgcggcatat cgaccccgtc gacgcacgga
     3301 tcgtcgacgt cctcgacgcc gcccgccggc tcgtcatgct cgaccgccgg cacctcgatc
     3361 gggtctccga ggcggggcac ctggcgacga cggagacgat gcaccggatc gctcgcgagg
     3421 tggccgagcg cgccggcgcc gatccgtccc gcgccgggcg gctgctcgcc gacactctcg
     3481 cgctggcgca ccgctgcgcc cagcatccgc gccacgatct cggcatcggc gcggtgcacc
     3541 tgcccgagcc gcacatcctc ggcatacccg agggcactga cccccagcgc gtcctcgacg
     3601 agcgctgcca cgcggcgatc ctcacccgct atccgggagc gagtgagagc gaacgccgcg
     3661 ccgtggaggc gcggctgcga gaagagctgg atgtcgttgc ggccctgggc tatccgacct
     3721 acttcatgac ggtcgcaacc gtcgtcgacc tgatccgcga gatgggggtg cgggtcgccg
     3781 cgcgcggctc cggcgccggc tcgctggtca actatctgct cggcatcagc ggcgtcgacc
     3841 cgatccgcta cgacctgctc atggagcgct tctgctcgcc gctgcgcgcc gaactgcccg
     3901 acatcgacat cgacgtcgag tccgctcgcc gcaccgatat ctacgagcag atcctcgacc
     3961 gcttcggcgg cgagcgggtg acctgcgtgt cgatgatgga cacctacaag gtgcgccatg
     4021 cgatccgcga tgtcggctcg gtgctcggta tgccgccggc cgagatcgac gagatcgcca
     4081 aggccttccc gcacatccgg gcccgcgacg ctcgacaggc catcgccgac atgcccgagc
     4141 tgcggcagcg cggtctcgat tccgaccagc tgacaacgct tttcgacctc gtcgagggtc
     4201 tcgacggcct gccacggcat atcgccctcc acccgtgcgg ggtgatcctc tccaatggca
     4261 cgctgctcga tcgcacgccg gtcgaggcga gctggctggg cttcccgatg agccagttcg
     4321 acaaggacga cgtcgaggcg gtcgggctgc tcaagctcga cgtcctcggc atccggatgc
     4381 agtcggcgat ggcccacgcg gtcgccgagg tggcccgggt cgacggcgtc accgtcgacc
     4441 tcgacgaccc ggcgcaggtg ccgctcgacg acgaggcgac ctacgcgatg atccgcacca
     4501 cccggaccct cggctgcttc cagatcgaga gcccgggcca gcgtgagctc atcggcaagc
     4561 tgggccccga gcgcttcgag gatctggtca tcgacatc
//
LOCUS       DAOPJG010000230         4567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_20673_length_4567_cov_8.306959, whole genome shotgun sequence.
ACCESSION   DAOPJG010000230 DAOPJG010000000
VERSION     DAOPJG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20673_length_4567_cov_8.306959"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PKU75_15270"
     CDS             complement(<1..1231)
                     /locus_tag="PKU75_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HPF82285.1"
                     /translation="MAGPQDVGPILNGIPTGLPDIDPDETQEWLDSLSAVIDEGGRAR
                     ARYLMLRLLEKSREMQVGVPSLTTTDYVNTIPIEHEPWYPGDEKVEKQIRRINRWNAA
                     MLVHRAQRPGIGVGGHISTYASAATLYEVGMNHFWRGAEHPGGGDQIFFQGHASPGMY
                     ARAFLEGRLTETQLDGFRQEHSHVVDGVPVALPSYPHPRLMPDFWQFPTVSMGLGPMN
                     AIYQAQFNKYLHNRGIKDTSQQRVWAFLGDGEMDEVESRGLLQTAANDELDNLTFVIN
                     CNLQRLDGPVRGNGKIMQELEAFFRGAGWNVIKVVWGRGWDPLLAADHDGALVNLMNQ
                     TPDGDYQTFRANDGKYVRDFFFGRDPRTRKLVEKWTDDEIWWNLKRGGHDYHKVYAAY
                     RAATEHEGQPTVILAKTI"
     gene            1392..1880
                     /locus_tag="PKU75_15275"
     CDS             1392..1880
                     /locus_tag="PKU75_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPF82286.1"
                     /translation="MTVALYDDTPGIVWRPVSPRLASARRIATVVTMAPFLLGALALA
                     IFVTRWSWVAVAVLLALLVLQFVLIGRQVSAISYAELPEELVIRRGRLFRRLVSIPYG
                     RLQYADMASGPLERHFDLASVTIHTASTASGGTIPGLPVAEAEDLRERLTARGEAQRA
                     GL"
     gene            1877..3487
                     /locus_tag="PKU75_15280"
     CDS             1877..3487
                     /locus_tag="PKU75_15280"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPF82287.1"
                     /translation="MTEPAIPDPTPPPEEEWQRFHPLTPLLRGGVVFMAIVGYVISTQ
                     TDRLFGSDPEDPTQGHLGIAAALAGVVLLAVIAGSWVIWLASRFRMGRTTLELKHGLV
                     MRQHRQVRYDRIQAVNITRPLLARLAGLSAVKVEAAGGAGSNIELAFLPQARAEDLRI
                     DLMRRAAVASGTVPASAVPGPAADGDPAGGAASGPAAGAPPGGAGAAGGEAAAYPTPA
                     GGLAPGERLVAAIPASRVWVATAMSFNTIFLVVTLPLLISALVLGHFSLVPVLGPMVL
                     ASLGQQVKRFTSWMNFRVEAGPDVVRIRHGLTELSTMSVPLHRIQAVEVSQPAMWRGL
                     GWWRINVNVAGVHREPDAAEESIIPVGTHEEVFAILAAMGPTWSLPEVGEGMYGEGPS
                     GVFVAAPERARRLDPLSWKRIGYAATPSVLLSRGGWLGRSVQIVPHARVQSLGLDQGM
                     IERRLRLANVHLHSTNGSVTPVVRHLDVDAATRLLNEEAARARDARHAEAGEPAADPL
                     GPKAPTDASGALSSLGEGTEGPRMDGWS"
     gene            3492..3914
                     /locus_tag="PKU75_15285"
     CDS             3492..3914
                     /locus_tag="PKU75_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HPF82288.1"
                     /translation="MNTPAAGGPSGLPKLGFSAGQVVQEYGYDDDVDHDFRYAVEDVI
                     GSELEYDDFTGVADGVLLWWRDGDGDLVDALVDVLTNLAEGAGIVVLTPKAGRVGEVD
                     AAEIEESAVTAGLHATANVSIGSDWSASRLLAPKSSRR"
     gene            3962..4456
                     /locus_tag="PKU75_15290"
     CDS             3962..4456
                     /locus_tag="PKU75_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPF82289.1"
                     /translation="MTASTPIAVGAPAPDFTLSTQDGNSRSLAEFRGSKNVLLVFYPF
                     AFSGICTGELTEIRDTLEDFEGDDVQVLCISCDPMFSLRAWADQQGYFFPLLSDFWPH
                     GAVAQAYGVFEESKGMAIRGTFLIDREGIVRWSLVNGPGEGRDFDGYRAALAQLRAGV
                     SRQA"
     gene            4494..4566
                     /locus_tag="PKU75_15295"
     tRNA            4494..4566
                     /locus_tag="PKU75_15295"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4527..4529,aa:Val,seq:tac)
BASE COUNT          636 a         1567 c         1622 g          742 t
ORIGIN      
        1 tgatggtctt ggcgaggatg accgtcggct ggccctcgtg ctcggtggcg gcgcggtatg
       61 cggcatacac cttgtgatag tcgtggccgc cccgcttgag gttccaccag atctcgtcgt
      121 cggtccactt ctcgacgagc ttgcgggtgc gcgggtcccg gccgaagaag aagtcgcgga
      181 cgtacttgcc gtcgttggcg cggaaggtct ggtagtcgcc gtcgggcgtc tggttcatca
      241 gattgaccag ggcgccgtcg tggtcggcgg ccagcagcgg atcccagccc cgcccccaga
      301 cgaccttgat gacattccag ccggcaccgc ggaagaacgc ctcgagctcc tgcatgatct
      361 tgccgttgcc gcggaccggt ccgtccaggc gctgcaggtt gcagttgatg acgaaggtca
      421 ggttgtcgag ttcgtcgttg gccgcggtct gcaacaggcc acgcgattcg acctcgtcca
      481 tctcgccgtc gccgaggaag gcccacaccc gctgctgcga ggtgtccttg atgccgcgat
      541 tgtggaggta tttgttgaac tgggcctgat agatcgcgtt catcgggccc aggcccatcg
      601 acacggtcgg gaactgccag aagtcgggca tcaggcgcgg atgcggatag gacggcagcg
      661 cgacgggcac gccgtcgacg acgtggctgt gctcctgccg gaacccgtcg agctgggtct
      721 ccgtcaggcg gccctcgagg aaggcgcggg catacatgcc gggggaggcg tgaccctgga
      781 agaagatctg gtcgccgccg ccggggtgct ccgcgccccg ccagaagtgg ttcatgccca
      841 cctcgtacag cgtggccgcg gacgcgtagg tggagatgtg gccaccgaca ccgatgccgg
      901 gccgctgcgc gcggtggacg agcatggcgg cgttccaccg gttgatgcgg cggatctgct
      961 tctcgacctt ctcgtcgccc ggataccacg gctcgtgctc gatcgggatc gtgttcacgt
     1021 agtccgtcgt cgtcagcgac gggaccccca cctgcatctc gcgggacttc tcgagcaggc
     1081 gcagcatgag atagcgcgcc cgcgctcgcc cgccctcgtc gatgacggcg gagagcgagt
     1141 cgagccactc ctgggtctcg tcggggtcga tatcgggcag tccggtggga atgccattga
     1201 ggatggggcc tacatcctgc ggtccggcca cggtgtgatc ctcctagatc ggcgtcggcc
     1261 tcggtgggcg tacccgccca tcctccaccc gaacgtggtg ccagtcacaa tccggactcg
     1321 catttttgga tggccggtcg atcccgctcg ccggtgcatc gctcccgcgg accaccaggc
     1381 agacttgggc catgacggtc gccctgtatg acgacacccc aggaatcgtg tggcgtccgg
     1441 tgtcgcctcg gctggcgagc gcgcgccgga tcgccaccgt ggtgacgatg gcgccgttcc
     1501 tcctcggcgc cctggcgctg gcgatcttcg tgacgcgctg gtcgtgggtc gccgtggccg
     1561 tgctcctcgc tctgctcgtg ctccagttcg tgctcatcgg ccggcaggtc agcgccatct
     1621 cgtatgccga gttgccggag gaactggtga tccgccgggg ccgcctcttt cgccggctgg
     1681 tgagcattcc ctacgggcgg ctgcagtatg ccgacatggc ctccggtccg ctggagcgcc
     1741 acttcgacct ggcgagcgtg accatccaca ccgcctcgac ggccagcggc ggcaccatcc
     1801 ccggcctgcc ggtcgccgag gccgaggacc tgcgcgagcg cttgaccgcg cgcggcgagg
     1861 cgcagcgggc gggcctgtga ccgagcccgc gatccccgat ccgacaccgc cgccggagga
     1921 ggagtggcag cggttccatc cgctgacccc gctgctgcgc ggtggcgtcg tcttcatggc
     1981 gatcgtcggt tatgtcatca gcacccagac ggaccgcctc ttcggttccg accccgagga
     2041 ccccacccag ggccacctcg gaatcgccgc cgccctggcc ggcgtcgtgc tcctggcggt
     2101 catcgccggc agttgggtca tctggctggc ctcgcgcttc cggatgggcc gcaccaccct
     2161 ggaactcaag cacggacttg tcatgcgcca gcaccggcag gtgcgctacg accgcatcca
     2221 ggccgtcaat atcacccgcc cgctcttggc ccggctcgcc gggctgtccg cagtcaaggt
     2281 cgaagccgcc ggcggtgccg gctccaatat cgaactcgcc ttcctgcccc aggcgcgcgc
     2341 cgaggatctg cggatcgacc tgatgcgccg cgcggcggtg gcgagcggca ccgtgcccgc
     2401 ctccgccgtc ccgggcccgg ccgccgacgg cgacccagcc ggaggcgcgg cgagcggccc
     2461 agcggcgggc gcgccacccg gaggcgcggg ggcagcggga ggcgaggcgg cggcataccc
     2521 gaccccggcc ggaggcctcg cccccggcga acggctcgtg gcggccatac cggcatcgcg
     2581 ggtctgggtg gcgaccgcga tgagcttcaa cacgatcttc ctcgtcgtca ccctcccact
     2641 gctgatcagc gccctggtgc tgggccactt cagcctcgtg cccgtgctcg ggccgatggt
     2701 gctggcgagc ctcgggcagc aggtcaagcg gttcacgtcg tggatgaact tccgcgtgga
     2761 agccgggccc gacgtggtgc ggatccggca cgggctgacc gagctgagca cgatgagcgt
     2821 gcccctgcac cggatccagg cggtcgaggt cagccagccc gcgatgtggc gcgggctggg
     2881 gtggtggcgg atcaacgtca acgtcgccgg cgtgcaccgc gaacccgatg cggcggagga
     2941 gtcgatcatc cccgtcggca cccacgagga ggtcttcgcg atcctcgccg cgatggggcc
     3001 gacgtggtcg ctgccggagg tgggcgaggg gatgtacgga gaggggccgt cgggcgtctt
     3061 cgtcgcagcg ccggagcggg cacggcggct cgatccgttg tcctggaagc ggatcgggta
     3121 tgccgcgaca cccagtgtcc tgctcagccg gggcggctgg ctgggccggt cggtgcagat
     3181 cgtgccgcac gcccgggtgc agtccctcgg tctcgaccag gggatgatcg aacggcggct
     3241 ccggctggcc aatgtgcacc tgcattcgac caacggctcg gtcaccccgg tcgtgcgaca
     3301 cctcgacgtc gacgcggcca cgcggctgct caacgaggag gcggcccgcg cccgcgacgc
     3361 ccgccacgcc gaggcaggcg aacctgcagc tgatccgctt ggcccgaagg ctcccaccga
     3421 cgcttccggc gcgctatcgt cccttggcga gggcacagaa ggaccccgaa tggatggatg
     3481 gagttaacgg cgtgaacacc cccgccgccg gcggcccgag cgggctgccg aagctcggct
     3541 tcagcgctgg gcaggtcgtg caggaatacg gctatgacga cgatgtcgac cacgatttcc
     3601 gctacgccgt cgaggacgtc atcggcagcg agctggagta tgacgacttc accggggtcg
     3661 ccgacggcgt gctgctgtgg tggcgcgacg gcgatggcga cctggtcgat gcgctcgtcg
     3721 acgtgctgac caatctcgcc gagggcgccg ggatcgtggt gctgacgccg aaggccgggc
     3781 gggtcggcga ggtggacgcg gccgagatcg aggagtccgc cgtcacggcc ggtctgcacg
     3841 ccaccgccaa cgtgagcatc ggcagcgact ggtccgccag ccgcctgctg gcccccaaga
     3901 gcagccggcg ctgacccggg ccggtgggca cgcggcatac cgtgctcggc atacttgagc
     3961 catgaccgcg tccacgccga tcgccgtcgg cgcccccgcg cccgacttca ccctgtccac
     4021 ccaggacggc aacagccgct cgctggcgga gttccggggc tcgaagaatg tgctgctcgt
     4081 gttctatccc ttcgcgttct cggggatctg cacgggcgag ctgaccgaga tccgcgacac
     4141 gctcgaggat ttcgagggcg acgacgtgca ggtgctgtgc atctcgtgcg acccgatgtt
     4201 cagcctgcgg gcgtgggccg accagcaggg atatttcttc ccgttgctgt cggacttctg
     4261 gccccacggg gccgtcgcgc aggcctacgg ggtgttcgag gagtccaagg gaatggcgat
     4321 ccgaggcacc ttcctgatcg accgcgaggg catcgtgcgc tggagcctgg tcaacggtcc
     4381 cggggagggc cgcgacttcg acggctaccg cgcggcgttg gcgcaattgc gtgccggagt
     4441 ttcccgccag gcctgacaca atggggcgcc ggacgccccg cgtggcgccc gaggggcctg
     4501 tagctcagtt ggtagagcgc cacgtttaca ccgtggatgt cgtcggttcg agcccggccg
     4561 ggcccac
//
LOCUS       DAOPJG010000231         4535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_20850_length_4535_cov_6.321205, whole genome shotgun sequence.
ACCESSION   DAOPJG010000231 DAOPJG010000000
VERSION     DAOPJG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20850_length_4535_cov_6.321205"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1642
                     /locus_tag="PKU75_15300"
     CDS             377..1642
                     /locus_tag="PKU75_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPF82290.1"
                     /translation="MRLILRTGMAAWVGLSVAACGVLPSKTTTVVITTGVPSGGEVTE
                     AETQSGSPESEEAEPERPDDTSTASTSAGPQTLQEALELVKPAVAKVNVSSWTSDSMT
                     ETSYCGVRTGTAFAVTPTLLVTAAHVVEGRDRVRVTSGLTTQPGAVVGYDRAKDVALI
                     QVEHELPGSLTLRTDVVAPGDPVATYGYADGKAASYVAGTVNRLDQKAQVGEYFMTRL
                     LEVDFAAQPGNSGGPVFTIEGGVVGLVDAGDDGPGHEYAVSAETIALLVAQWAGNPTR
                     TDEAETSCQYLANFEAGPDTEPTSEAVESAALTLMTYYQAVNDADYSSALAQLANPGS
                     LEAFQDGVETSEISNLEYLSTMEEAGEPVIEVSFTSRQEAGKGPKDRPDETCTQWRIR
                     YQFEPRDNMWLIGKVGPLLWESQADSPCY"
     gene            complement(1692..2768)
                     /locus_tag="PKU75_15305"
     CDS             complement(1692..2768)
                     /locus_tag="PKU75_15305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPF82291.1"
                     /translation="MPDWVLHLDMDQFIAAVEIRRRPELAGLPVIVGGRGDPSERAVV
                     STASYEARAFGARSGMPLRLAVRKCPDAVILPVDGPAYDAASAEVFAAVRALGLTVEV
                     LGWDEAFIAVPDTGDPEVAARRIQAAVLDATALHCSIGIGDNRIRAKNATDFGKPRGT
                     FRLTRDNWMQVMGHRPVIELWGVGTRISTRLGSHGIQTVAELAAADRDDLITEFGPKM
                     GTWYAALGRGEGSAVVDDTPWVARSHSRETTFQENLKTPEEVRAAVEVLTDQVLADCA
                     AEGRPIVRVGLKVRYAPFFTVNRMKKLPEPTHDRAPVLGAVLALVGGMEEGREVRLLG
                     VRGEMAMPEEPDDPVERTPTRGRV"
     gene            2840..3085
                     /locus_tag="PKU75_15310"
     CDS             2840..3085
                     /locus_tag="PKU75_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82292.1"
                     /translation="MYDVTMRTTVDLPPAVHARARELAARRGQSLSAVVADLAIRGLA
                     QLDEPVRLAINPQTGLPVISLGRRITSAEVDAILDDE"
     gene            3082..3474
                     /locus_tag="PKU75_15315"
     CDS             3082..3474
                     /locus_tag="PKU75_15315"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPF82293.1"
                     /translation="MTTFLLDANVLIAVSLAEHEHYARASAWFGAQPRVAICPSVEGA
                     LLRFLIRLGERPSAAHQILVALANHPRIEFWPDATSYSDVDLTGLQGHRQVTDFYLIE
                     VARRRNGLLATLDSRLVAERPDDAVLIP"
     gene            3539..>4535
                     /locus_tag="PKU75_15320"
     CDS             3539..>4535
                     /locus_tag="PKU75_15320"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HPF82294.1"
                     /translation="MAHYTSMGAVPPKRHTQHRDSAGNLYYEELMGEEGFSSDSSLLY
                     HRRIPSEISAAEVWQVPDQTLTPNHPLKPLHLKLRDLFPDGGPGVDAVTGRRLILGNS
                     DVRISYVVAESPSPFYRNAIGDELVYIERGSAQVDTVFGAFEVGRGDYVVIPRATTHR
                     WLPKPPGDDADHEPLRAYCIEAASHITSPKSYRSRFGQLLEHSPYCERDLRLPEGPLL
                     AEDIGADAGDETQVYVKHRGPGPGGIAGTIYTHPFHVLDVVGWDGCLYPYVFNISDFE
                     PITGRVHQPPPAHQVFEGRNVVVCNFVPRKVDYHPLAIPVPYYHSNVDSDEVMFYV"
BASE COUNT          784 a         1548 c         1466 g          737 t
ORIGIN      
        1 cgctgtgtcg agcgggctgt tgccgggcgg gaacacggcg gcgtcgtcga agaggctggc
       61 gaagagcgcg cgtggcagtg acacgcgccc caacctatcc gctgggaacg ggaggcgacg
      121 tgtggtcacc gtcccggtaa acgcaaggct cccaactcgt aaccgttgat tcggtcgggc
      181 gcgcaacgca atcggcaact tggcgagcaa ctttgggtgg gcaaacgtgg gactagcctt
      241 tggcgaggaa ccggcgaagc gctcaggcct ggccgcacgc gggaacgcac agatgagtcc
      301 ctccattgga ggacaagccg cagaacgatc acgtgcgtaa cctgagtcac attcgtcacg
      361 tgcgtgggga gtttgcatgc gtttaatcct gagaaccggc atggccgcct gggtcggact
      421 gagcgtcgcc gcctgtggcg tccttccgag caagaccacc accgtggtca tcaccacggg
      481 cgttccctct gggggtgagg tcacggaggc cgagacgcag agcggttctc cggagtcaga
      541 ggaggccgag ccggagagac ctgatgacac gagcacggcg tcaaccagcg ccggccccca
      601 gacgctgcag gaagcactgg agttggtcaa gcccgcagtc gccaaggtga atgtctcgtc
      661 atggacctcg gactccatga cggagacctc ctactgcggt gtccgcactg gcacggcgtt
      721 cgccgtcact cccaccctgc tggtcaccgc cgcacacgtc gtcgaggggc gtgaccgggt
      781 ccgcgtcacg agcggcctca cgacccaacc cggagccgtg gtcggctacg accgcgccaa
      841 ggacgttgcc ttgatccagg tcgagcacga actgcctggg tcgctgacgc tgcggactga
      901 cgtcgtggcg cctggtgacc cggttgcgac atatgggtat gccgatggca aagcggcctc
      961 gtatgtcgcc gggaccgtca accggctcga ccagaaggcg caggtgggcg aatacttcat
     1021 gacccgcctg cttgaagtcg attttgccgc gcaacccgga aatagcggtg gcccggtgtt
     1081 caccattgag ggtggcgtcg tggggttggt cgatgccggc gacgacggcc ccgggcatga
     1141 gtatgccgtt tcggccgaga caattgctct gctcgtcgcg cagtgggctg ggaatccgac
     1201 gcggacggac gaggccgaga cgtcatgcca gtacctggcc aacttcgagg cagggcccga
     1261 tacggaaccg acatccgagg ccgtggagag tgcggcgctc accctaatga cctactacca
     1321 ggccgtcaac gacgccgact attcatccgc gctcgcgcag ctcgcgaatc ccggttctct
     1381 cgaggcgttc caggacggag tcgagacttc cgagatctcc aacctggagt acctctcgac
     1441 gatggaagag gcaggcgagc cggtgattga ggtgtccttc accagccgcc aggaggcggg
     1501 caaaggcccc aaggatcgcc cggatgagac ctgtactcaa tggcgcatcc gctaccagtt
     1561 cgaaccacgc gacaacatgt ggctgatcgg caaagtcggt cccctcctgt gggagagcca
     1621 ggccgactca ccctgctact gaggctcggt ctgcgccgat ccggagcaca ccgactatga
     1681 gtagtcactg ttcagacgcg gccgcgggtg ggagtgcgct cgacggggtc gtcgggctct
     1741 tcgggcatgg ccatctcgcc gcggacgccg aggaggcgga cctcgcgccc ctcctccatc
     1801 cccccgacca gggccaggac ggcgccgagc accggcgccc gatcgtgcgt cggctcggga
     1861 agcttcttca tccggttcac ggtgaagaag ggcgcgtagc gaaccttcag cccaacccgc
     1921 acgatgggcc ggccctcggc ggcgcaatcg gcgagcactt ggtcggtcaa cacctccacg
     1981 gccgcgcgca cctcttcagg cgtcttgagg ttctcctgga aggtcgtctc ccggctgtgg
     2041 ctgcgcgcca cccagggcgt gtcatcgacg accgcagagc cctcgccgcg gccgagcgcg
     2101 gcataccagg tgcccatctt gggtccgaac tcggtgatga gatcgtcgcg gtcggcggcg
     2161 gcgagctccg ccacggtctg tatgccgtgg ctccccagcc gcgtcgagat ccgcgtgccg
     2221 acaccccaga gttcgatgac ggggcggtgg cccatcacct gcatccagtt atcgcgcgtg
     2281 aggcggaagg tgccgcgcgg cttgccgaag tcggtggcgt tcttggcccg aatccggttg
     2341 tccccgatgc cgatcgagca gtgcagtgcc gtcgcgtcga ggacggctgc ctggatccgg
     2401 cgtgccgcga cctccgggtc gccggtgtcc ggtaccgcga tgaatgcctc gtcccatccg
     2461 agcacctcga cggtcagtcc gagcgcccgg accgcagcga agacctccgc cgacgcggcg
     2521 tcatacgccg gcccgtcgac agggaggatc acggcgtcgg gacacttgcg cacggccagc
     2581 cgcagcggca tacccgaccg ggcgccgaag gcgcgcgcct catagctggc ggtcgagaca
     2641 acggctcgtt cactcgggtc gccgcgcccg ccgacgatga ccggcaggcc ggccagctcg
     2701 gggcgccgcc ggatctcgac agccgcgatg aactggtcca tgtcgagatg gagcacccag
     2761 tccggcatgt ccccatcatc gaggatcggg ccgctggacg agcgcttagg ctggcaaacg
     2821 cacgcatcac ctcagcatca tgtacgatgt gaccatgcgt accacagttg atctgccgcc
     2881 tgccgttcac gcccgtgccc gtgagcttgc tgcccgtcgg ggccaatctc tttcggccgt
     2941 cgttgctgat ctcgcgatcc gagggcttgc gcaactggat gaaccggtga ggctggcgat
     3001 caatccgcaa actggcctac cggtgatttc gctcggccgc cgcatcacca gtgcggaagt
     3061 cgatgcgatc ctggacgacg aatgacgact ttcctcctgg acgcaaacgt cctgatcgcc
     3121 gtatcgctgg cagagcacga gcattacgca cgggcgtcgg cgtggtttgg tgcacaacca
     3181 cgcgtagcca tctgcccgag cgttgagggc gcactcttgc gatttctcat tcgtctaggg
     3241 gaaaggccgt ccgcagccca ccagatcctt gtagcgctgg cgaaccatcc gcgaatcgag
     3301 ttctggccgg atgcgacctc gtactccgac gtggacctaa cgggtctgca aggtcatcgg
     3361 caagtgacgg acttctatct catcgaggtc gcccggcgcc gcaatgggct gttggcgacc
     3421 ttggactcgc gactggtagc agagcgtccg gatgacgcag tcctcatccc ctgacgcgac
     3481 cccggatagg gtggccggac cggggagacc cctcgggaca atcccaacga ggtgagacgt
     3541 ggcgcactac acgagcatgg gcgcggtgcc cccgaagcgg catacgcagc atcgcgactc
     3601 cgccggcaac ctctactacg aagagctgat gggggaggag ggtttctcct ccgactcctc
     3661 gctgctctat caccggcgca tcccgagcga gatcagcgcc gccgaggtgt ggcaggtccc
     3721 cgaccagacc ctcacgccga accatccgct caagccgctg cacctgaagc tgcgcgacct
     3781 cttccccgac ggagggccgg gcgtcgatgc ggtcaccgga cgtcgcctga tcctcggcaa
     3841 cagcgacgtg cggatctcgt atgtcgtggc ggagagcccg agtccgttct accgcaacgc
     3901 gatcggcgac gaactcgtct acatcgagcg ggggagcgcg caggtcgaca cggtcttcgg
     3961 ggccttcgag gtcggccgcg gcgactatgt cgtcatcccg cgcgccacca cccaccgctg
     4021 gctgcccaaa cctcccggcg acgacgccga ccacgagccg ctgcgcgcct actgcatcga
     4081 ggctgccagc cacatcacct cgcccaagag ctaccgaagc cgcttcggcc agctgctcga
     4141 gcactcgcca tactgcgagc gcgacctgcg cctgccggag ggcccgctgc tcgcagagga
     4201 catcggtgcc gacgccggcg acgagaccca ggtctatgtc aagcaccgcg gccccggccc
     4261 gggcggcatc gccggcacga tctataccca ccccttccac gtcctcgacg tcgtcgggtg
     4321 ggacggctgc ctctatccct acgtcttcaa catcagcgac ttcgagccga tcaccggccg
     4381 ggtccaccag ccgccgcccg cccaccaggt cttcgagggc cggaacgtcg tcgtctgcaa
     4441 cttcgtgccc cgcaaggtcg actatcaccc cctcgccatt ccggtgccct actaccactc
     4501 caatgtcgac tccgacgagg tcatgttcta cgtcg
//
LOCUS       DAOPJG010000232         4529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_20883_length_4529_cov_7.836835, whole genome shotgun sequence.
ACCESSION   DAOPJG010000232 DAOPJG010000000
VERSION     DAOPJG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20883_length_4529_cov_7.836835"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..795
                     /locus_tag="PKU75_15325"
     CDS             118..795
                     /locus_tag="PKU75_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF82295.1"
                     /translation="MISDDRRILVALVNDYEVVVKGLGRMFESHSDEVEVVEYNVKTS
                     VSRPVDIALFDTFAQSPDGASSHIDDVLADPDVARVVLYSWNLDPDAVAAGMDRGVHG
                     YVSKTLSTAELIEQLRRVKSGEYVVPDAPAPPGDGDADSPTNGDWPGREAGLTARESE
                     IIALITTGLSNNDIARQTYLSINSVKSYIRSAYRRMGVSSRSQAVLWGVQHGLAPQAP
                     ERQHPAR"
     gene            complement(957..1496)
                     /locus_tag="PKU75_15330"
     CDS             complement(957..1496)
                     /locus_tag="PKU75_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPF82296.1"
                     /translation="MQFGRSYEEFEVGATYKHWPGKTVTEFDDHLFCLLTMNHHPLHM
                     DAHYAEETTQFGKNVVVGNYVYSILLGMSVPDISGKAIANLEVESLRHVAPTFHGDTL
                     YGETTVLDKWESSSKDDRGVVYVESIAYNQDGKVVCIFRRKVMVPKANYLEARGGEQP
                     GRPVPQPDKNWPGPEAAEK"
     gene            complement(1599..2735)
                     /locus_tag="PKU75_15335"
     CDS             complement(1599..2735)
                     /locus_tag="PKU75_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82297.1"
                     /translation="MSTDQTTFDPAVQDQLHTELSYADLPGFDTELLLAQGHRAARRR
                     TMSRVAGATAAVVALAIGGYAVAGNLGLGNGSTPPATGTSTTVDATPVTAKLEPATSL
                     AENGQTIDPGRYGRLAVTWDPAPKTAGPDLTYSTYAEDGTLTTIGGSSTQGLAPNAVS
                     WGTSGPDSHVIVGVLPRSAQRFSVVLPQDPQGGHSSTSAQSALPGSPWQAFGVFFADG
                     GDVDRIVDILWQDTSGRIFDKDGSEVPSVSLGDKAGSRVYLAQRAGVMGIFTADGDHT
                     SSWLDDTDGAARLHPVLDIGRGSAATMDGFFAMLVPAGSSKPQLTPGAKVLGADDPTI
                     TQIPGSGQAVLWAHYTATGTSSAPSFTTISWVGPDGTPARVTKY"
     gene            complement(2732..3244)
                     /locus_tag="PKU75_15340"
     CDS             complement(2732..3244)
                     /locus_tag="PKU75_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HPF82298.1"
                     /translation="MFGPSKATRDAEFTAFVASAQQPLLRMAYALTGEAEAARDLVQE
                     ALTKTYVAWGRIRSDEAMAYTRRIIVNQRIDTWRKTRKETVTGTLPDTAVDSREGVAD
                     RRDALVRALAALPEQQRKVVVLRHYADMSEAHVADTLGISRGAVKSAASRGLAALRQH
                     ASSTEEGLLR"
     gene            complement(3320..>4529)
                     /locus_tag="PKU75_15345"
     CDS             complement(3320..>4529)
                     /locus_tag="PKU75_15345"
                     /inference="COORDINATES: protein motif:HMM:NF020119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPF82299.1"
                     /translation="IDWLAGRGHAVDARGRTVRPDGFRASVGMVGTSYDGTLPNAVAT
                     TGVPELKAIIPISAISDWYDYYRSGGAVVAPQSYQGEDADVLAKFVLTRANRAICAPI
                     IKDLGRRQDRRTGDRNAFWQERNYRARAGRVTAAVYAAHGLSDWNVKPSQAGRWYRAL
                     RAGGVPVKIYWHPGGHGGPPPQREQNRWFTHFLMGVANGVEREPRALVETVDGSLERY
                     ADWPVPGSRPRTTSLSQWRIPGQPQTVTRLSFRDDTAYTLLGGQLPRRGRGLVFTSDP
                     LTEPTRLSGFARADLRIKLGQPNANVTVGIVELPGGAFSPLVTMGWTDPQNRHGEWRT
                     EAVTPGEWMNVDVDLEASEHVFQAGSRIGIVVIQSDHEFTIRPPMAKTMTLDTRSSTI
                     TLPLTRALPR"
BASE COUNT          657 a         1486 c         1601 g          785 t
ORIGIN      
        1 tcgctcatgg cgccatggtg tcacgcaaac cgcgccccta cggggacacc cttaggatgg
       61 attgcaggtt ctcggtccgt tatccgtccc tgtgtgccgg ggcgctacga tcaggaggtg
      121 atatcggacg atcgtcggat ccttgtggcc ttggtcaacg actacgaagt ggtcgtcaag
      181 ggcctcggcc gcatgttcga atcgcactcc gacgaagtcg aggtcgtcga gtacaacgtc
      241 aagaccagcg tgagccgccc cgtcgatatc gcccttttcg acacctttgc ccagtcgccc
      301 gacggggcct cctcgcacat cgacgacgtg ctcgccgatc ccgatgtcgc gcgtgtcgtg
      361 ctctattcgt ggaatctcga tccggatgct gtcgcggcgg gtatggaccg cggcgtgcac
      421 ggttacgtga gcaagacgct ctcgaccgcg gaactgatcg agcagttgcg gagggtgaag
      481 tccggtgagt acgtcgtgcc cgacgccccc gccccgccgg gcgatggcga cgcagactcg
      541 cccacgaacg gggattggcc cggccgcgag gcgggattga ccgcccgcga gtccgagatc
      601 atcgccttga tcacaacggg cttgtccaac aacgacattg cgcgccagac ctacctgtcg
      661 atcaactccg tcaagtccta catccgcagt gcctaccgcc gcatgggcgt cagcagccgc
      721 agccaggcag tcctctgggg cgtccagcac ggattggccc ctcaggcgcc cgagcgtcag
      781 cacccggcgc gctgagcttc tcgggcggcc ccgcgtccgg tcacccctgt gggtgtggca
      841 tcggcacgaa atatgttcat ggccctgctg ctccgcaccc gacggcggtc tcgccccggg
      901 ggtcttgctc cccgtagtcg ggagccaggt gagcggacgc gctggacgca gcagggctat
      961 ttctcggcag cctcggggcc gggccagttc ttgtccggct gcgggaccgg gcggcccggc
     1021 tgctcgccgc cacgggcctc gagatagttc gccttgggca ccatcacctt gcggcggaag
     1081 atgcacacga ccttgccgtc ctggttgtag gcgatggact cgacatagac cacgccccgg
     1141 tcgtccttgg acgaggactc ccacttgtcg agaacggtgg tctccccata gagcgtgtcg
     1201 ccgtggaagg tcggagcgac gtgccgcagc gactcgacct ctaggttggc gatggctttg
     1261 ccggagatgt ccggcacgga catgccgagc aggatcgagt agacgtagtt gccgacgacg
     1321 acgttcttgc cgaattgcgt tgtctcctcg gcgtaatggg cgtccatgtg cagtgggtgg
     1381 tggttcatcg tcagcaggca gaacaggtgg tcgtcgaact cggtgaccgt cttgccgggc
     1441 cagtgcttgt aggtcgcgcc gacctcgaac tcctcgtagc tgcgtccgaa ctgcatgctg
     1501 cgtccactcc ttcgtgcctg ctgtgggtat gccgttccca tcctccccgg tcggcctcgc
     1561 gcgggggagg ccggccggtg gtgggttccc tcacaggctc agtacttcgt cacccgggcc
     1621 ggcgtgccgt ccgggccgac ccagctgatc gtggtgaacg acggtgcgga tgaggtcccg
     1681 gtggcggtgt agtgcgccca caggacggcc tggccgctcc cggggatctg ggtgatcgtc
     1741 ggatcgtcgg cgccgaggac cttggcgccc ggcgtcagct gtggcttgct cgacccggcg
     1801 gggacgagca tggcgaagaa cccgtccatc gtggcggccg agccgcgtcc gatgtcgagg
     1861 accggatgca ggcgcgcagc accgtccgtg tcatccagcc acgaggaggt gtggtcgccg
     1921 tccgcggtga agatccccat gactccggct cgctgcgcga ggtaaacccg cgaaccggcc
     1981 ttgtctccca acgagactga cggcacttcc gatccgtcct tgtcgaaaat ccgcccggac
     2041 gtgtcctgcc acaggatgtc gacgatccga tccacgtcgc cgccgtcggc gaagaagacc
     2101 ccgaacgcct gccacgggga tccgggcagt gcgctctgcg cggaagtcga gctgtgcccg
     2161 ccctgggggt cctgggggag cacgacggag aagcgttgtg ctgaccgtgg cagcacaccg
     2221 acgatcacgt gactgtccgg gcccgaagtt ccccacgaga cggcattcgg agcgagcccc
     2281 tgagtgctcg agccgccgat cgtggtcaga gtgccgtcct ccgcataggt cgagtacgtc
     2341 aggtccggac cggccgtttt cggtgccggg tcccaggtca cggcgagccg gccgtagcgg
     2401 cccggatcga tggtctgtcc gttctccgcg agcgaggtcg ccggctcgag tttggcggtc
     2461 accggcgtcg cgtcgaccgt cgtgcttgtg cctgtcgccg gcggcgtgga accgttgccg
     2521 aggccgaggt tgccggcgac ggcatacccg ccgatcgcga gggcgaccac ggcggcagtg
     2581 gcgccggcca cgcggctcat cgtgcgtcgc cgggcggcgc gatgtccttg cgcgagaagc
     2641 agttcggtgt cgaacccggg gagatctgca tacgacagct cggtgtgcag ctgatcctgg
     2701 acggccgggt cgaaggtggt ctggtcggtg ctcatcgcag cagtccttcc tcggtgctgg
     2761 acgcgtgctg tcggagtgcg gcaagcccgc gagaggccgc ggacttcacg gcgccccggg
     2821 agatgccgag ggtgtcggcg acgtgcgcct cgctcatgtc ggcgtagtgg cgcagcacga
     2881 cgaccttgcg ttgctgctcg ggcaaggcgg ccagggcgcg caccagggcg tcgcgccgat
     2941 cggcgacgcc ctcgcgagag tcgaccgcgg tgtcggggag ggtgccggtc accgtctcct
     3001 tgcgggtctt gcgccaggtg tcgatgcgtt ggttgacgat gatgcgccgg gtgtaggcca
     3061 tggcctcgtc gctgcggatg cgaccccacg ctacatacgt cttggtgagc gcctcctgga
     3121 ccaggtcccg ggcggcttcg gcctcgccgg tcaaggcgta ggccatgcgc agcagcggct
     3181 gctgcgccga ggccacgaac gcggtgaact ccgcatcgcg ggtggccttc gaaggtccga
     3241 acatcgcgct cgcccttcct ttctcatcgg tcaccggcgt acacggaccg cggcgctgaa
     3301 aggttgcctc gtcccggaac tatctcggca gggcgcgggt cagcgggagg gtgatggtgc
     3361 tggagcgcgt gtccagcgtc atggtcttgg ccatcggggg gcggatggtg aactcgtggt
     3421 cgctctggat gacgacgatc ccgatgcggg agccggcctg gaagacgtgc tcgctcgcct
     3481 cgaggtccac gtcgacgttc atccactccc cgggcgtgac ggcctcggtg cgccactcgc
     3541 cgtggcggtt ctgcggatcg gtccagccca tggtgaccag cggcgagaag gcgccgcccg
     3601 ggagttcgac gatgccgacg gtgacattgg cgttcggttg gccgagcttg atccgcaggt
     3661 cggctctcgc gaagccggac agccgggtcg gctcggtcag cgggtcgctc gtgaacacga
     3721 gcccccgccc gcgcctggga agctgtccgc ccagcagggt gtatgccgtg tcgtcgcgga
     3781 acgacaggcg ggtaacggtc tggggttggc cgggaattcg ccactgcgac aacgatgttg
     3841 tgcgcggccg gctgccgggc acgggccagt cggcataccg ctccaggctg ccgtcgacgg
     3901 tttcgacgag cgcgcgcggc tcgcgttcga caccattggc gacacccatg agaaagtggg
     3961 tgaaccagcg attctgctca cgctggggcg gcgggccacc atggccgcca ggatgccagt
     4021 agatcttcac cggcactccg ccggcgcgca gcgcgcggta ccaccggccg gcctggctgg
     4081 gcttgacgtt ccagtcgctc aggccgtgcg cggcatacac ggccgcggtg accctcccgg
     4141 ctcgtgcgcg atagttgcgt tcctgccaga acgcattgcg atcgccggtg cggcggtcct
     4201 gccggcgccc gaggtccttg atgatggggg cgcagatggc ccgattcgcc cgggtgagaa
     4261 cgaatttggc caggacgtcg gcgtcctcgc cctgatagga ctgcggcgcc accactgcgc
     4321 cgccggagcg gtaatagtcg taccagtcgg agatcgcgga gatcgggatg atcgccttga
     4381 gctccggaac ccctgtggtg gcaacggcat tcggcagcgt gccgtcatag gacgtgccga
     4441 ccatgcccac cgaggcgcgg aagccgtccg gccgcacggt gcggccccgg gcatcgacgg
     4501 cgtggccacg cccggcgagc cagtcgatg
//
LOCUS       DAOPJG010000233         4457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_21362_length_4457_cov_8.666970, whole genome shotgun sequence.
ACCESSION   DAOPJG010000233 DAOPJG010000000
VERSION     DAOPJG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21362_length_4457_cov_8.666970"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PKU75_15350"
     CDS             <1..822
                     /locus_tag="PKU75_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPF82300.1"
                     /translation="PKNAYFTAPDGKTLATGWKENVGTKNFKTLLTNETIRAGFLKIF
                     LWNVAFAAISVLSTFLLGMLLALLFNDPKLKGKGFYRSLLILPYAIPGFVTALVWASM
                     FNQEFGLVNALTHLNVDWLGNAWAAKAAILITNLWLGFPYMFLVCTGALQSIPSDVKE
                     AAKIDGAGAFRTLRSITMPLLLVAVGPLLIASFAFNFNNFSLIYLLTQGGPFQSGDTS
                     IGSTDLLITYAFRLAFSGASPNIGLASAVCIFIFFIVALMSYSGFRRTAQLEEVN"
     gene            825..1742
                     /locus_tag="PKU75_15355"
     CDS             825..1742
                     /locus_tag="PKU75_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF82301.1"
                     /translation="MSNVTDAAAVAIPEGRAATSKAPRGVGGSTWWRHLLGVLALIWA
                     LFPIVFLVSAALNPAGSLQTSSLIPKEMSMGNFDTLFNDASRPYTSWYKNSMIISLVG
                     SLGSVFIGACAAYAFSRMRFAGRRPGLLALLLLQMFPAMLTFPALYLTFAKLGDIIPA
                     LGLNSIWALILAYLGGAMGANVWLLKGYFDTIPKELDEAAKVDGASHARIFFTMTLRL
                     VMPILVTVFMLSFVGLFSEFLLASIFLQDVDSQTLGVGLYSMSIGNEKNRLFGQFAAG
                     ALLASLPVVLLYLGFQKFLIGGMTQGSVK"
     gene            1739..3691
                     /locus_tag="PKU75_15360"
     CDS             1739..3691
                     /locus_tag="PKU75_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPF82302.1"
                     /translation="MSPAAHLLDTPHHDGSLRYLSQPHPRLGETVDLYVRVPHAASVT
                     SLHVRTTHDAEPKFTAGVVDRHTASDTWWRVPLVVHNPVAHYRFLLQGGELGYQWLNG
                     TGLHERDVPDAADFRITTYDPPPAWSDDAVVYQIFPDRFARSASADARPTPDWAEPVS
                     WDTPVAGRGRRTGRQLYGGDLEGIAEHLDHIETLGATVVYLTPVFPSRSNHRYDATTF
                     DRVDPILGGDEALTKLAAAVHARGMRILGDFTTNHTGAGHEWFRAAQADPAAPERDYY
                     FFDGDRYVAWLDVKSLPKLNYDSAELIRRVFDDPQGVVRKWLTGPDGLDGWRIDVANM
                     TGRYGAQDRYHDVARRMREAATTARPDALLVAEHCHDTSRDALGDGWHGVMNYAGFTR
                     PLWTWLRHREYAPDFLGLPVIVPRLGGRAVMETMREFAAQMPWVTLTHSFNQVGSHDT
                     TRVRTLVGDDAGQVDVAAGMLLTMPSIPMITYGDEIGMLGEFGEDGRRPMPWDAAPGR
                     GGRGGTDPAAPGGEPPATTPGGAPPATTPAGEPPLWDERLHTVYRDLIAMRRASPALH
                     AGGLRWVYADDDVLVYLREHPQQTALVHLARLAHDPVRLPAAALDGIESGCAAYGPGL
                     VVDGDEIVLSAGGPHVGVWTWSPRAE"
     gene            3670..>4457
                     /locus_tag="PKU75_15365"
     CDS             3670..>4457
                     /locus_tag="PKU75_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF82303.1"
                     /translation="MVPPRGVKLRLAEVARQAEVSESTVSRVLNDRPGVSETTRAAVL
                     TAIDVLGYEVPQRLRPRAGALVGLITPELTNPIFPAFAQTIESGLAVRGFTPVLCTQT
                     PGGVHEDVYVQMLLDRGVAGIICVSGQHADTLESVERYVSLLNHGVPLVLVNGYREGL
                     DAAFISTDDQVAMEESITHLRELGHERIGLAVGQRQYVPVQDKVAAFTRLMGPGAADL
                     VAYGLFTVEGGALTTSALIERGATAIVYGSDIMALGGMRQARRLG"
BASE COUNT          652 a         1628 c         1432 g          745 t
ORIGIN      
        1 ccgaagaacg cctacttcac cgctccggac ggcaagaccc tcgcgaccgg atggaaggag
       61 aacgtcggca ccaagaactt caagaccctg ctgaccaacg agacgatccg cgccgggttc
      121 ttgaagatct tcttgtggaa tgtcgccttc gccgcgatct cggtgctgtc gaccttcctg
      181 ctcggcatgc tgttggcgct gctgttcaac gacccgaagc tgaagggcaa gggcttctat
      241 cgctcgctgc tgatcctgcc ctacgccatc cccggcttcg tcaccgcgct cgtgtgggcc
      301 tcgatgttca accaggagtt cggcctggtc aacgccctca cccacctgaa tgtggactgg
      361 ctcggcaacg cctgggcggc caaggcggcc atcctcatca cgaacctgtg gctgggcttc
      421 ccgtacatgt tcctggtgtg caccggcgcg ctgcagtcga tcccgagcga tgtcaaggag
      481 gccgcgaaga tcgacggtgc gggtgccttc cggaccctgc gctcgatcac gatgccgttg
      541 ctactggtcg cggtcggccc gctgctgatc gcctccttcg cgttcaactt caacaacttc
      601 agcctgatct acctgctcac ccagggcggg ccgttccagt ccggtgacac gagcatcggc
      661 tccaccgacc tgctgatcac ctacgccttc cggctcgcgt tctccggcgc cagcccgaac
      721 atcggcctgg cctccgcggt ctgcatcttc atcttcttca tcgtcgcgct catgagctac
      781 tcgggcttcc ggcgcaccgc gcaactggaa gaggtcaact gaccatgtcg aatgtcaccg
      841 atgccgctgc cgtcgcgatc cccgagggtc gcgccgccac ttccaaggcc ccgcgtggtg
      901 tgggggggag cacctggtgg cgccacctgc tgggcgtcct cgccctgatc tgggcgctct
      961 tcccgatcgt cttcctcgtc tcggcggcgc tcaaccccgc cggttcgctg cagaccagtt
     1021 cgctgatccc caaagagatg tcgatgggca acttcgacac cctgttcaac gacgcctccc
     1081 ggccctacac cagctggtac aagaactcga tgatcatcag cctggtgggg tcgctgggct
     1141 cggtcttcat cggggcctgc gcggcatacg ccttctcgcg gatgcgcttc gccggccgcc
     1201 gtcccggcct gctcgccctg ctgttgctgc agatgttccc ggcgatgctg accttcccgg
     1261 cgctctatct caccttcgcc aagctcggcg acatcatccc ggcgctcggc ctgaactcga
     1321 tctgggcgct catcctcgcc tatctcggcg gggcgatggg tgccaacgtc tggctcctca
     1381 agggctactt cgacacgatc cccaaggaac tcgacgaggc cgccaaggtc gacggcgcca
     1441 gccacgcgcg gatcttcttc acgatgacat tgcgtctggt catgccgatc ctcgtgaccg
     1501 tcttcatgct cagcttcgtc ggcctgttca gcgagttcct cctcgcgagc atcttcttgc
     1561 aggacgtcga ttcccagacg ctgggtgtcg ggctctacag catgtcgatc ggcaacgaga
     1621 agaaccgcct cttcgggcag ttcgccgcgg gcgcgctgct cgcgtcgctg ccggtggtgc
     1681 tgctctacct cggcttccag aagttcctga tcggcggtat gacgcagggt tcggtgaagt
     1741 gagccccgcg gctcacctcc tcgacacccc gcaccacgac ggttcccttc ggtacctctc
     1801 ccagccgcac ccgcggctgg gagagaccgt cgacctctac gtgcgggtgc cgcacgcggc
     1861 gtcggtcacc agcctgcacg tgcggaccac gcacgacgcc gagccgaaat tcactgcggg
     1921 agtggtggat cggcataccg ccagcgacac gtggtggcgg gtgccgctcg tcgtgcacaa
     1981 ccccgtcgcg cactaccgct tcctgctcca aggcggcgaa ctcggctacc agtggctcaa
     2041 cggcaccggc ctgcacgagc gcgacgtgcc cgacgccgcg gacttccgga tcacgactta
     2101 cgatccgccg ccggcgtgga gcgacgacgc cgtcgtctac cagatcttcc ccgatcgctt
     2161 cgcccgctcg gcgtccgccg acgcccggcc cacgcccgac tgggccgaac ccgtctcgtg
     2221 ggacaccccg gtcgcgggcc gcggccgccg caccggacgg cagctgtatg gcggcgatct
     2281 cgaggggatc gccgagcacc tcgaccacat cgagaccctc ggcgccaccg tcgtctatct
     2341 gacgccggtc ttccccagcc gctccaatca ccgttacgac gcaacgactt tcgaccgtgt
     2401 cgacccgatc ctgggcggcg acgaggcact gaccaagctc gcggcggcgg tgcacgcgcg
     2461 cggcatgcgc atcctcggcg acttcacaac caaccacacc ggagccggcc atgagtggtt
     2521 ccgcgccgcc caggccgatc cggctgcgcc ggaacgggat tactacttct tcgacggtga
     2581 ccgctatgtc gcatggctcg acgtcaagtc gcttccgaag ctcaactacg actcggccga
     2641 gctgatccgg cgggtcttcg acgacccgca gggcgtggtc cgcaaatggc tgaccggtcc
     2701 cgacggactc gacggctggc gcatcgacgt cgccaacatg accggccgct acggcgccca
     2761 ggatcgctat cacgacgtgg cgcggcggat gcgggaggcc gcgacgaccg cccgccccga
     2821 cgcgctgctc gtcgccgagc actgccacga cacctcccgc gacgcgctcg gcgacggttg
     2881 gcacggggtg atgaactacg ccgggttcac ccggccgctg tggacgtggc tgcgccaccg
     2941 cgagtatgcg ccagacttcc tcggcctgcc cgtcatcgtg ccccgcctcg gcggccgcgc
     3001 cgtcatggag acgatgcgcg agttcgccgc gcagatgccg tgggtcacgt tgacgcacag
     3061 cttcaaccag gtcggctccc acgacacgac ccgggtgcgc accctcgtcg gtgacgacgc
     3121 cggccaggtc gacgtcgccg cggggatgct gctgacgatg ccgagcatcc cgatgatcac
     3181 ctatggcgac gagatcggga tgctcggtga gttcggcgag gacgggcgcc ggccgatgcc
     3241 ctgggacgcc gcaccgggcc gaggcggccg aggcggcacc gacccagccg cccccggcgg
     3301 tgagccgccc gccaccactc ccggcggtgc gccgcccgcc accacccccg caggcgagcc
     3361 gccgctgtgg gacgagcgac tgcataccgt ctatcgcgac ctgatcgcga tgcggcgcgc
     3421 ctccccggcc ctgcacgccg gcgggctgcg gtgggtgtac gccgacgacg acgttctggt
     3481 ctacctgcgc gaacacccgc agcagacggc cctggtgcac ctggcccggc tcgcccatga
     3541 ccccgtgcgg ctccccgcgg cggcgctgga cggcatcgag agcgggtgcg cggcatacgg
     3601 tccggggctc gtcgtcgacg gcgacgagat cgtcctgtcc gccggcggac cgcacgtggg
     3661 agtgtggaca tggtcccccc gcgcggagtg aagctgcgcc tggcggaggt cgcccgccag
     3721 gcggaggtca gcgagtcgac ggtgagccgg gtcctcaacg accggccggg tgtctccgaa
     3781 acgacccgcg ccgccgtgct caccgccatc gacgtgctcg ggtatgaggt gccgcagcgc
     3841 ctgcgcccgc gcgccggggc gctcgtcggg ctgatcacgc ccgaactgac gaatccgatc
     3901 ttccccgcgt tcgcccagac catcgagtcg gggctggcgg tgcgcgggtt caccccggtg
     3961 ctgtgcacgc agaccccggg cggggtgcac gaggacgtct atgtgcagat gctcctcgac
     4021 cggggcgtcg ccggcatcat ctgcgtctcc ggccagcacg cggacacgct cgagagcgtg
     4081 gaacggtatg tctcgctgct gaaccatggc gtgccgctgg tgctggtcaa cggttatcgc
     4141 gagggcctcg acgccgcctt tatctccacc gacgaccagg tcgcgatgga ggagtcgatc
     4201 acccacctgc gcgagctcgg ccacgaacgc atcggcctgg ccgtgggcca gcggcagtat
     4261 gtgccggtgc aggacaaggt ggcggcgttc acgcggctga tggggcccgg cgccgcggac
     4321 ctggtcgcct acgggctgtt caccgtcgaa ggtggtgccc tgaccacctc ggccctgatc
     4381 gagcggggcg cgacggcgat cgtctacggc tcggacatca tggccctcgg cggtatgcgg
     4441 caggcccgcc ggctcgg
//
LOCUS       DAOPJG010000234         4405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_21673_length_4405_cov_7.180230, whole genome shotgun sequence.
ACCESSION   DAOPJG010000234 DAOPJG010000000
VERSION     DAOPJG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21673_length_4405_cov_7.180230"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PKU75_15370"
     CDS             complement(<1..482)
                     /locus_tag="PKU75_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPF82304.1"
                     /translation="MQVVVLADTHAPRRWKGIPEGLGAPLSRAEVILHAGDVCTPDVL
                     DALAGFAPVHAVLGNNDGPAVAAWGAPESLELVLGGVRMAMIHDSGARQGRGRRMRRR
                     FPDADLVVFGHSHIPFDGVEDGVHLFNPGSPTDRRRQPAGTYGVLQLADGVVVRHEIV
                     P"
     gene            complement(602..2182)
                     /locus_tag="PKU75_15375"
     CDS             complement(602..2182)
                     /locus_tag="PKU75_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPF82305.1"
                     /translation="MDYGHELEFGAFLTPDAAHLDQLLENAQLADVLGLDLVCLQDHP
                     YAAQQAEMWTLLSVIGARTSAVRLAPNVASLPLRPPVVLAKAAATLDRITGGRVEIGL
                     GAGAFWDGIAAAGGPRRTPKDAVDALIEAIEILRAFWTGGTVRHDGTHYRTVGLRAGP
                     TPAHEIPLWLGAYKPRMLRVTGRLADVWIPSMGYADPPALADMNAVIDEATIAAGRAP
                     ESVRRAYNIVGSFGAATGFLQGTPRDWAEQLADLTLTQGMATYILGSDDPRALATFAQ
                     EVAPAVRDLVEAERARAATRSEPGGIPGSVAPQGNPPAEPSEPGEGSPRHTGLGVRPT
                     PDPGERLAREQAWAESDRPAFAMPEREYTAHEEATAAHLVDVHDHLRSELTQIRDIVA
                     QVRSGAMGIGSARSAINTMTLRQNNWTLGAYCAAYCRILTGHHTLEDRSIFPHLRRAE
                     PGLAPVIDRLEREHEVIHDLLERLDEALVAMVTADGMPPALAELSGALDLLTDALLSH
                     LSYEERQLLAPLAAHGFG"
     gene            complement(2219..2479)
                     /locus_tag="PKU75_15380"
     CDS             complement(2219..2479)
                     /locus_tag="PKU75_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82306.1"
                     /translation="MTKQESETRAAVAAVTLAAVHRRDHRHAGGADCMPHCTQVVHLG
                     QRAAMVCHDCATDSGFVDSRAAEELARRHTEATRGREPRPAA"
     gene            complement(2847..>3188)
                     /locus_tag="PKU75_15385"
     CDS             complement(2847..>3188)
                     /locus_tag="PKU75_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82307.1"
                     /translation="ATAPSSVCFFAAAGNFGFTGTYASLVADSLALADVLLDEAGDDE
                     LVGAALVLVTDAVAADVVGPEPPSHPASRAPHTTRTTQAPARALPFRAIPNLPTGGFP
                     GAAHDVSWPPP"
     assembly_gap    3189..3288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3289..3678
                     /locus_tag="PKU75_15390"
     CDS             <3289..3678
                     /locus_tag="PKU75_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82308.1"
                     /translation="TLIRLDNYFAIKWSEQGYEPEEIDSLNPADWFGLTVRIKPQFKS
                     VVYDFGDGTSFGPTTDLGGPYPTGNIVKAYADAGVYNTQIHATLTGQVSLNGSEWIDI
                     PGQADLDGPVTPLTVLTADNRLYASGG"
     gene            complement(3827..>4405)
                     /locus_tag="PKU75_15395"
     CDS             complement(3827..>4405)
                     /locus_tag="PKU75_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82309.1"
                     /translation="GVSAAVTAAAARRLALVRATTDETNSLLPGDELVDRPTIVATRG
                     IDIAAPPEAVWPWVAQLGQERGGFYTYAWLENLVGCRMVNADRINPQWQDVAVGDIVR
                     LHPDLGLRVAVVEPGRTLTLTGDGAVDRNGERWPTRLRLLVDLHGHPDGGGVSPADQG
                     AVPSAHETSSCHDQPGRPAQHDHVRGHAARHP"
BASE COUNT          611 a         1485 c         1587 g          622 t
ORIGIN      
        1 ggcacgatct cgtgccgcac gaccaccccg tcggcaagct gcagcacccc atacgtcccc
       61 gccggctgcc gccggcgatc cgtcggcgac cccgggttga acaggtgcac cccgtcctcg
      121 accccgtcga acgggatgtg cgagtgcccg aacaccacca gatccgcgtc ggggaaccgg
      181 cgccgcatac gacgccccct gccctgccgc gcgcccgagt cgtgaatcat cgccatccgc
      241 actccgccca gcaccagctc gagcgactcg ggcgcacccc acgcggccac cgccggcccg
      301 tcgttgttgc cgagcacggc gtgcaccggc gcgaacccgg cgagcgcgtc cagcacgtcc
      361 ggcgtgcaca catcccccgc gtgcaggatc acctccgccc gcgacagcgg cgcgcccagc
      421 ccctcgggaa tgcccttcca ccgccgcggc gcgtgcgtat cggccaagac cacaacttgc
      481 atgggctcag tctcccccga cgacatggcc cgacccaggg cgcggcgact agctcagcgc
      541 gcacgccgtg ccgcgtgccc tccgcgcgcg ccacacgcac gcgagaggat gccccaggcg
      601 gtcacccgaa cccgtgcgcg gcgagcggcg cgaggagctg ccgctcctca tacgacaaat
      661 gcgacagcag cgcatcggtc agcagatcca gcgcgccgga cagctcggcg agggcgggcg
      721 gcataccgtc cgcggtgacc atcgcgacca gcgcctcatc gagccgctcc agcaggtcgt
      781 ggatgacctc gtgctcgcgt tcgaggcggt cgatgaccgg cgcgaggccg ggctcggcgc
      841 gccgcaggtg cgggaagatg ctgcggtcct cgagggtgtg gtggccggtg aggatccggc
      901 agtacgccgc gcaatacgcc ccgagcgtcc agttgttctg ccgcagcgtc atcgtattga
      961 tcgccgagcg cgccgacccg attcccattg cgccagaacg cacttgggcc acgatgtcgc
     1021 ggatctgggt cagctcgctg cgcaggtggt cgtggacgtc gaccaggtgc gcggcggtcg
     1081 cctcctcgtg cgctgtgtac tcccgctcgg gcatcgcgaa ggccggccga tcgctctccg
     1141 cccaggcctg ttcgcgggcg agccgctccc cggggtcggg ggtgggccgg accccgaggc
     1201 cggtatgccg cgggctcccc tcccccggct cgctcggctc ggccggtggg ttgccttggg
     1261 gggcaacgct tcccggtatg ccgcccggct cgctcctcgt cgcggctcgg gcgcgttcgg
     1321 cctcgacgag gtcgcggacg gcgggggcga cctcctgggc gaaggtcgcg agggcgcggg
     1381 ggtcgtcgct gccgaggatg taggtggcca tgccctgggt gagggtgagg tcggcgagtt
     1441 gctcggccca gtctctcggg gtgccttgga ggaaacccgt tgcggcgccg aaggatccga
     1501 cgatgttgta ggcgcggcgc accgactccg gggcgcggcc ggcggcgatc gtggcctcgt
     1561 cgatgacggc gttcatgtcc gcgagggcgg gcggatcggc ataccccatc gacgggatcc
     1621 agacgtcggc gaggcggccg gtgacgcgca gcatgcgcgg cttgtaggcg ccgagccaga
     1681 gggggatctc gtgcgccggg gtggggccgg cgcgcaggcc gacggtgcgg tagtgcgtgc
     1741 cgtcgtggcg gacggtgccg ccggtccaga acgcgcgcag gatctcgatg gcttcgatga
     1801 gggcgtcgac ggcgtccttg ggggtgcggc gcgggccgcc ggcggcggcg atgccgtccc
     1861 agaaggcgcc ggcgcccagg ccgatctcga cgcggccgcc ggtgatccga tcgagggtcg
     1921 cggcggcctt ggcgaggacg acgggcgggc gcagcgggag ggaggcgacg ttcggggcca
     1981 gccgcacggc gctggtgcgg gccccgatga ccgacagcag ggtccacatc tcggcctgct
     2041 gcgcggcata cggatggtcc tggaggcaga ccaggtcgag gccgaggaca tcggcgagtt
     2101 gcgcgttctc cagcagctgg tcgaggtgcg cggcgtcggg ggtgaggaac gccccgaact
     2161 ccaactcgtg cccgtagtcc atgcagggca ttctcgccga ggtggcgcgg gtgtggggtc
     2221 aggcggcggg gcgcggttcg cggccgcggg tggcctcggt atgccgccgg gccagctcct
     2281 ccgccgcgcg cgagtcgacg aacccggagt cggtggcgca gtcgtggcaa accatcgccg
     2341 cgcgctggcc gagatgcacg acttgggtgc agtgcggcat acagtcggcg ccgccggcgt
     2401 gccggtggtc gcggcggtgg accgccgcga gcgtcacggc cgcgacggcg gcgcgggtct
     2461 cgctctcttg cttggtcatc acaaccccct cattggtgcc tttgcaccat gagacgcccg
     2521 gatgcgccga ttggataccg gtgatccgga aaatgtggat aactcggggg ccactcccgg
     2581 gtcgaccctg atcactggcg ggcgactggg cgtccggggg tggctgaggt ccggcttcgc
     2641 gtggcgatcg tcggacgacg cggtgggcgt cgtcgttgac gctgtcgtcg ttgacccgcg
     2701 gtagtcgcca gcctatgcct gggtgcgggc ggacgcgagg ggtctggggg cgccagcggc
     2761 ggcgtggcgg ggatcacaag tcggtcacat ccggagcgcg gctgtggata agagggcgtt
     2821 ccggtggcga gatcgggcga cctgggtcaa ggtggaggcc aggacacgtc gtgggcggcc
     2881 cccgggaagc cgcccgtcgg gaggttggga atggctcgaa atggcagcgc ccgcgcaggg
     2941 gcttgcgtgg tcctcgtggt gtgtggcgcc ctgctggctg ggtgtgacgg cggctcgggt
     3001 ccgacgacgt cggcggcgac ggcgtcggtg acgagcacga gtgcggcccc gacgagttcg
     3061 tcgtctccgg cctcgtcgag cagcacgtcg gcgagcgcca gcgagtccgc gacgagcgag
     3121 gcgtatgtgc cggtgaagcc gaagttccct gcggcggcga agaagcagac cgacgagggg
     3181 gcagttgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac actgatccgg
     3301 cttgacaact acttcgctat aaagtggagc gagcagggat acgagccgga ggagatcgac
     3361 agcctcaacc cggccgattg gtttgggctg acggtgcgca tcaagccaca gttcaagtcc
     3421 gtcgtctacg acttcggcga cggcacctcg ttcgggccca ccaccgacct tggcgggccg
     3481 tacccgaccg gcaacatcgt gaaggcttac gccgacgcgg gcgtctacaa cacccagatc
     3541 cacgcgacac tcaccggcca ggtctccttg aacggctcgg agtggatcga catccccggg
     3601 caagccgacc tcgacgggcc ggtcacgccg ttgaccgtgc tcaccgcgga caaccggctg
     3661 tacgcctcgg gcggctgact ggcggccgtt cgccatcaca ctcgccgcca cgtcccgcca
     3721 tcagcgtcac gtgagaggta gcgacatggg aacgtcgcta actcttacgt gaggatcgtc
     3781 gaccttcgtc cgatcccacc gggcggatcc ggcacgctgg gcccgatcac ggatgccgcg
     3841 cagcatgccc tcggacatga tcgtgctgag ccggacgccc aggttggtca tgacacgagc
     3901 tggtctcgtg cgcgctcggt accgctccct ggtcagcagg cgacaccccg ccgccgtcgg
     3961 gatgaccgtg aaggtccacg agtagtcgta gccgggtcgg ccaccgctcc ccgttccggt
     4021 cgacggcgcc gtcgcccgtc agggtcaggg tccgaccggg ttcgacaacc gcgacccgca
     4081 gtcccaggtc aggatgcaga cgcacgatgt ccccgaccgc gacgtcttgc cactgggggt
     4141 tgatgcgatc ggcattgacc atccggcatc cgaccaggtt ctcgagccag gcataggtat
     4201 agaagccccc gcgctcctgc cctagctggg ccacccacgg ccacaccgcc tccggcggcg
     4261 cagcgatgtc gatgccccgg gtcgccacga tcgtgggccg gtcgaccaac tcgtcaccgg
     4321 gaaggagcga gttcgtctcg tccgtcgtgg cgcgcaccag cgcgagacgg cgcgccgccg
     4381 cggcagtgac cgccgccgac actcc
//
LOCUS       DAOPJG010000235         4389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_21793_length_4389_cov_8.257730, whole genome shotgun sequence.
ACCESSION   DAOPJG010000235 DAOPJG010000000
VERSION     DAOPJG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21793_length_4389_cov_8.257730"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..2387
                     /locus_tag="PKU75_15400"
     CDS             54..2387
                     /locus_tag="PKU75_15400"
                     /inference="COORDINATES: protein motif:HMM:NF017369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HPF82310.1"
                     /translation="MSATRPQTGSSLHDGSPLLRLLCALVLALAVAGSGVPSSRAAGP
                     ETGQPFPARLVLGISDPSSPHKPEISRRLSIGGVTIEVSPALTQGIPGGEVTAYVTGA
                     AGTDTRAAGAALRAGTASVTRLAPRDDGRTALGLHHVTVVPVYWTAKPTSDAEIVSAV
                     TGSMTKVAAYFDTATQSQIRFTTDRVFGWNKISLTAAEIESCDYEAMERKARAVAPST
                     PKDERHHFVVMLEPTPTCAWGGRGTVGPGELGDLVTWLNYQPYEGPTYAHEFGHNLGL
                     WHTGYLQCWNDTRTANVTLSANCDQRTYNDPWDLMSAPLNWPGMLATINRQRIGVLPV
                     TDNPLIMDSQRVTLKPVAAGSGVRGFQLQAGNVQYFVEYRTPVGLDDWIDDSTYVDPK
                     GVTRTDPGGGVTVRRQDDFFGQFGEIDLLDFHPDANPLSYQWHPGLEAGESYTIADHG
                     ITLSVVSTSSTAAVVDVTITQDDGVVRWDGRDRYAASAAISARTFPANTWLAYVASGE
                     VFSDALSGAPVAGRDGAPLLLTRRDSLPATIAAELQRLKPSTIVVLGGPATVSESVVD
                     GLRAYASETVERRSGADRYAASAAISRAAFLSTVDAAFVASGEIFSDALSGAPVAARA
                     PGPMLLTRPDGIPAPIAAELRRLKPKRIVIVGGPASVSEQVRAQLAEYTAGTVSRWFG
                     SDRYAASAAISQHGYPRPPVETAYVASGSIFTDALSGAPVAGMTAGPLLLVRTDSIPD
                     PIAAELSRLRPTKIVVLGGPASVSPGVEAALATFVRP"
     gene            2460..2972
                     /locus_tag="PKU75_15405"
     CDS             2460..2972
                     /locus_tag="PKU75_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82311.1"
                     /translation="MSESSAQTTIGAWFAERIPAEIYAGALSVTVDAQEIVVIGDVPR
                     TDGDPEPPVERIAAHRERTRAGRIAIAQDAEATFGRKVSWGARADGTERLYTHLGVPV
                     MTRLRQPERLLLDTLVEGGVARSRSEALAWCARLVGERESEWLAELHDALAGVRDARS
                     RNPFDRDTAS"
     gene            complement(2985..3236)
                     /locus_tag="PKU75_15410"
     CDS             complement(2985..3236)
                     /locus_tag="PKU75_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82312.1"
                     /translation="MSKHISRRDDVNPDEGEREHGDVTFADPTNNKYPIDSEEHVRAA
                     WSYINKKANADKYAADEVETIKGRIKRAAKKYDIEISDD"
     gene            complement(3371..4327)
                     /locus_tag="PKU75_15415"
     CDS             complement(3371..4327)
                     /locus_tag="PKU75_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82313.1"
                     /translation="MSLHDDFLVLNRADLDGLNLTWAEVMDVIDDAFVQKARGEVQNP
                     PKPKVAPRSDAFVNAMPAFLAGSDSLGIKWVSGFEQNRTKDLPYIYGALLMNDADTGR
                     PLALIDGGWVTEKRTAAVSGVTLRHVPGSVGTLGIIGAGLQGNRHLEVALEVHPEIEH
                     VRVFDHREAHGHALLAQAGERVGTVVHSALEATQDADLVISSISRRMDPRLDGSNTAP
                     DALILPVDYDDTFGPDVVREAAFFAVDDRGQYDSVLHRAFQGYRDPDGELAQLVSGVM
                     TVPDHGRRVFTNMGLAMEDVALGALILERATATRAGTTITFP"
BASE COUNT          669 a         1554 c         1468 g          698 t
ORIGIN      
        1 cgcggcgcac ctgacggtgg tggccgccgg ccacgctgga acggaggagc gtcatgtcag
       61 ccaccaggcc gcagacaggt tcatctctcc acgacgggtc accgctgctg cggctgctgt
      121 gcgccctcgt gctcgcgctt gcggtggccg ggagcggggt gccgtcgagt cgcgcggcag
      181 ggccggagac cgggcagccc ttccccgcca ggctggtgct cggcatcagc gatcccagct
      241 cgccgcacaa gccggaaatc agccggcgac tgagcatcgg tggcgtgacg atcgaggtga
      301 gtcccgcact cacgcaaggg atcccggggg gcgaggtgac ggcatacgtc accggcgccg
      361 ccggcacgga cacccgagcg gcgggggcag ccctgcgcgc gggcaccgcg tccgtcaccc
      421 gactggcgcc gcgcgacgac ggccggaccg cgctcggcct gcatcacgtg acggtggtgc
      481 cggtctattg gacggccaag cccacgagcg atgccgagat cgtctcggct gtcacgggga
      541 gcatgacgaa ggtcgcggca tacttcgaca cagcgacgca gagccagatc cggttcacca
      601 ccgacagggt gttcggctgg aacaagatca gcttgacggc tgctgagatc gagtcgtgcg
      661 actacgaagc gatggagcgc aaggcgcgcg ccgtcgcgcc gtcgacgccg aaggacgagc
      721 gccaccactt cgtcgtgatg ctggagccga cgccgacctg cgcgtggggt ggccggggca
      781 ccgtcgggcc cggcgaactc ggcgatctgg tcacctggct gaactaccag ccctacgagg
      841 gcccgaccta cgcccacgag ttcggccaca atctcggcct gtggcacacg ggatatctgc
      901 agtgctggaa cgacactcgc accgcgaacg tcaccctgtc ggccaactgc gaccagcgga
      961 cctacaacga tccctgggac ctgatgagcg ccccgctcaa ctggccgggc atgctcgcca
     1021 ccatcaacag gcagcgcatc ggcgtgctgc cggtcacgga caatccgctg atcatggaca
     1081 gtcagcgcgt cacgctcaag cccgtggccg ccggctccgg cgtccgcgga ttccagctgc
     1141 aggcgggcaa cgtgcagtac ttcgtcgaat accgcacccc cgtcggcctc gatgactgga
     1201 tcgacgacag cacctacgtc gatcccaagg gggtgacgcg cacggatccc gggggtggcg
     1261 tgaccgtgcg acgtcaggac gacttcttcg gccagttcgg cgagatcgat ctgctcgact
     1321 tccacccgga cgcgaatccg ctttcatacc aatggcatcc gggtctcgag gcgggtgaga
     1381 gctacacgat cgccgaccac gggatcaccc tctcggtcgt cagcaccagc tccaccgccg
     1441 cggtcgtcga cgtcacgatc acccaggacg acggggtcgt gcgctgggac ggccgggacc
     1501 ggtatgccgc gtccgccgcc atctcggcac gcaccttccc cgccaacacc tggctggcct
     1561 atgtcgcgtc cggcgaggtc ttcagcgacg ccttgtccgg agcacccgtc gccggccgcg
     1621 acggcgctcc gctgctgctg accagacgcg actccctccc cgccacgatc gcggcggagc
     1681 tgcagcggct gaagccgtcg acgatcgtcg tgctcggcgg gccggccacg gtctccgaga
     1741 gcgtcgtcga cgggctgcgg gcgtatgcct cggagacggt ggaacggcgc tcgggagcgg
     1801 accggtatgc cgcgtccgcc gccatctcgc gcgccgcctt cctcagcacg gtggacgcgg
     1861 cgttcgtcgc cagcggtgag atcttcagcg acgccctcag cggcgcaccc gtcgctgccc
     1921 gcgcgcccgg gccgatgctg ctgactcggc cggacggcat acccgccccg atcgccgccg
     1981 agttgcgccg cctgaagccg aagcgcatcg tcatcgtcgg cggaccggcg agcgtgagcg
     2041 agcaggtgcg ggcccagctg gcggagtaca cggcggggac cgtcagccgc tggttcggca
     2101 gcgaccggta tgccgcgtcc gccgcgatct cgcagcacgg ttatccccgg cccccggtcg
     2161 aaactgctta tgtggcaagc ggatccatct tcacggatgc cctgtccgga gccccggtcg
     2221 ccgggatgac cgccggtcct ctgctgctcg tacgcaccga ctcgatcccg gacccgatcg
     2281 ccgccgagtt gagccgcctg cggccgacca agatcgtcgt gctcggcggg cccgcgtccg
     2341 tctcgcccgg cgtcgaggcc gcccttgcga ccttcgtgcg cccctgagac gacccacgac
     2401 gtccggacgg atcacgcgtc gcacttgcgg tgtatgtcac gtaatgatgt aatcacgcta
     2461 tgagtgaaag cagcgcccaa accacgatcg gcgcttggtt cgccgagcga atcccggccg
     2521 agatctacgc cggcgctctc tcggtcacgg tcgacgctca agagatcgtc gtcatcgggg
     2581 acgtcccccg caccgacggt gatccggaac cgcccgtcga gcgcattgcc gcccatcggg
     2641 agcgcacccg cgcggggcgc atcgccatcg cccaggatgc cgaggccacg ttcggacgca
     2701 aggtgtcctg gggtgctcgc gccgacggca ccgagcgcct ctacacccac ctcggcgtcc
     2761 cggtcatgac ccgactccgc cagcccgagc gcctcctgct cgacacgctc gtcgaaggcg
     2821 gagtcgcccg ctcacgctcg gaggccctcg cctggtgcgc gcgcctcgtc ggggagcgcg
     2881 agtcggagtg gctcgccgag ttgcacgacg ccctcgccgg cgtccgagat gcgcgctctc
     2941 gcaacccgtt cgaccgcgac accgcctcgt gaccaacgtc gagatcagtc gtcggagatc
     3001 tcgatgtcgt actttttggc cgcgcgtttg atgcgtccct tgatcgtctc gacctcgtcg
     3061 gcggcgtact tgtccgcgtt cgccttcttg ttgatgtagg accacgccgc ccgcacgtgt
     3121 tcctcggagt cgatggggta cttgttgttg gtcgggtcgg cgaaggtcac atcgccgtgc
     3181 tcccgctcgc cctcgtcggg gttgacatcg tcacgccggg agatgtgctt gctcatgatt
     3241 cgacggtatg gcagttcggt cccggcgatc ggattggcgc ccaccgatga ccgcccagcg
     3301 cccggccgtc atgccttgag catgttctcg atgtattgcg catgcaccgg agcaggtgca
     3361 ccggcgcagg tcacgggaag gtgatcgtcg tgccagcgcg ggtggccgtc gcgcgttcga
     3421 ggatcagggc gccgagggcg acgtcctcca tggcgaggcc catgttcgtg aagacgcgcc
     3481 ggccgtggtc cggaaccgtc atcacccccg acaccagctg cgcgagttcg ccgtcggggt
     3541 cgcggtagcc ctggaacgct ctgtgcagca ccgagtcgta ctggccgcgg tcatcgactg
     3601 cgaagaaggc ggcctcccgc acgacgtcgg gcccgaacgt gtcgtcatag tcgaccggga
     3661 ggatgagcgc gtcgggtgcg gtgttgctgc cgtccagccg cgggtccatc cggcggctga
     3721 tcgaggagat caccaagtcc gcgtcctgcg tggcctccag cgcggaatgc acgaccgtgc
     3781 cgacccgttc gccggcctgg gccaacaggg cgtggccgtg cgcctcgcgg tggtcgaaga
     3841 cacgcacgtg ttcgatctcc ggatgcacct ccagggcgac ctcgagatgc cggttgccct
     3901 gcaggcccgc cccgatgatg cccagtgtcc ccacggaccc gggcacatga cgaagcgtca
     3961 ccccggacac ggccgccgtc cgcttctcgg tcacccatcc gccgtcgatg agggcgagcg
     4021 gtcgcccggt gtcggcgtca ttcatcagga gggccccgta gatatagggc aggtccttgg
     4081 tgcggttctg ctcgaacccc gaaacccatt tgatgccaag cgaatccgag ccggcgagga
     4141 aggccggcat cgcgttgacg aaggcgtccg accgcggcgc caccttgggt ttcggagggt
     4201 tttgcacctc cccacgcgcc ttctggacga aggcgtcgtc gatcacgtcc atgacctcgg
     4261 cccacgtgag gttcagtccg tcgaggtccg cccgattgag tacgaggaag tcgtcgtgaa
     4321 gactcatata tcgacagtag ttcgccggtc acagctgggc catatcgccc ggcgccctat
     4381 cccgctggt
//
LOCUS       DAOPJG010000236         4304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_22381_length_4304_cov_7.479407, whole genome shotgun sequence.
ACCESSION   DAOPJG010000236 DAOPJG010000000
VERSION     DAOPJG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22381_length_4304_cov_7.479407"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..2899)
                     /locus_tag="PKU75_15420"
     CDS             complement(146..2899)
                     /locus_tag="PKU75_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPF82314.1"
                     /translation="MRRHPRARWLLPLVLVLASLLAAGQSLAARDTRPPTAPSGLTAV
                     STTTGVTLTWKASTDNVGVKFYVVTVATQTKRPTAPKAAFANLRPNTSYTARVQAVDA
                     AGNRSTAVRLAFRTKARDTTPPSAPGAITTAPEITGATISWGAASDNIGVTAYVLTVD
                     GRSQTLTARTATVTGLRPNTSYLASVLAKDAAGNTSPAVSATFRTLPEPDVTPPSVPG
                     SPTVTPGSTQATLNWTPSTDNVGVSGYVVAVAGLAEQTVTATTATLDGLTPDTTYTAT
                     IVATDAAGNRSAAAEATFTTLPPPDTTAPSEPTALAVTGQSTTTGVVTWTAATDDVAV
                     TGYLVTAAGRTQEVSGTSATVTDLPVGTTTAVAVVAVDAAGNRSTAGRINVRTLLEPD
                     TQAPSSPEVSVVPGSTRAAVTVAEARDDVGVVRYVVTVAGQSLSITEPGTLTVTGLLP
                     ARDHQLRVTARDASGNVSPVTIVAFPTLPAGATPDGTPPTPPTNLSVRAGSSTLQLTW
                     SPASDNVDVDRYVVTVGDIIREESGHTLTLDGLAPQTPYDVEVVAVDTSGNASAPVTG
                     QAVTRPGPSNAQGLRGTIAWFGKNTSRATMDEALASPLVDGLSVYLRWSDITTDGVTF
                     NWSLFETARQAASAAGKPWNGMIIYGVVGNGMPAYVTDGLPAGQLITIDGETFPTFWS
                     PQAQAAARDLVTAAAQRFGSDPNLVQWRVTGLWSVNAEPRILGGAPGRRTWVDTYRIE
                     HPGATFEDVQAAYNDYEKHIWAEAAAAWPTRITLAQAGGLALADNVNTLPNTDPAKHP
                     QRLATWGEIRARLGARMVGQFNGVDAGSGAGGFGEWLPIAFGPNGFYPGRVGAQSIGG
                     VSQDTRLTADDFREMVRLLTVRGYSYGELYGSDVIYALRGRNAEALHMRATLEMYHDG
                     WTP"
     gene            3042..3554
                     /locus_tag="PKU75_15425"
     CDS             3042..3554
                     /locus_tag="PKU75_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPF82315.1"
                     /translation="MSDERREVAVGEWIAATRAAREEGFDFFDWLSAVDRTSEEPAGF
                     DVVVHLYDVSTPGALRGLLVQTRIVEGEALPSLTGVFAGAAWHERETHEMFGIDVAGF
                     DDGSGLGLRPLLLPDGFEGHPLRKDFYLAARVSKDWPGAKEPGQPHTGGPGRRKMQPP
                     GVPPQEWGPR"
     gene            3551..>4304
                     /locus_tag="PKU75_15430"
     CDS             3551..>4304
                     /locus_tag="PKU75_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPF82316.1"
                     /translation="MSILEIVLRAAAALAMLLVLPLVVGQTEHKVMAHMQGRLGPMYA
                     GAYHGWAQLVADGAKFVQKEDLVPRAADRGVFKLAPALAMIPYLLGLAAIPIHPGYAA
                     IEAPASVLFVLAATSIGVLGTLMAGWASANKYALLGAMRGAAQLISYELPMVLAVASV
                     ALAAGTLSLPGVIEAWTPWWLLWQLPGAFVFLAAAVAELQRPPFDMPIADSEIVFGPY
                     TEYTGLRFALFLLSEYAGIVLFSLLFAVLYLGG"
BASE COUNT          559 a         1376 c         1652 g          717 t
ORIGIN      
        1 tttaggttgg ggaccggcgg gggcggcgcg gtagccgtct tttcggagga ccgtcacgga
       61 ccatgcgcga cgtccgacga atcgcgacct ctgacttgtc agatctcggg cgttgtcggg
      121 gttgggcggg ctcctggtct cgccgtcagg gggtccagcc gtcgtggtac atctccaacg
      181 tcgcccgcat gtgcaacgcc tcggcgttcc ggccccggag ggcgtagatg acgtcactgc
      241 cgtacaactc gccgtaggag tagccgcgca cggtcaacag gcggaccatc tcccggaagt
      301 cgtcggcggt gaggcgggtg tcctgggaaa ccccgccgat cgattgggcg cccacgcgac
      361 cggggtagaa gccgttgggg ccgaacgcaa tgggcagcca ttcgccgaac ccgcccgctc
      421 cagatcccgc gtcgacgcca ttgaactggc cgaccatccg cgcgccgagc cgggcgcgga
      481 tctcgcccca cgtcgccaac cgttgcgggt gtttcgccgg gtcggtgttg gggagggtgt
      541 tgacattgtc ggcgagggcc aggccgccgg cctgcgcgag tgtgatccgc gtcggccacg
      601 ccgccgctgc ctcggcccag atgtgcttct cgtagtcgtt gtacgccgcc tgcacgtcct
      661 cgaaggtggc gcccgggtgt tcgatccgat aggtgtccac ccacgtgcgg cgacccgggg
      721 ccccaccgag aatgcgcggc tccgcgttga cggaccacag gccggtgacc cgccactgga
      781 cgaggttggg gtcggagccg aagcgctgcg cggccgctgt gaccagatcg cgagccgccg
      841 cctgagcctg tggggaccag aaggtcggga aggtctcgcc gtcgatggtg atcagctgcc
      901 cggcgggcag gccgtcggtg acataggccg gcatcccatt gcccacgacg ccgtagatga
      961 tcatcccgtt ccacggcttg ccagccgcgc tggcggcctg acgggcggtc tcgaagaggc
     1021 tccagttgaa ggtgaccccg tccgtggtga tgtcgctcca gcgcaggtag acggacaggc
     1081 cgtccacgag tggagacgcg agggcttcgt ccatggtggc ccggctggtg ttcttgccga
     1141 accacgcgat cgtgccgcgc agcccctggg cgttgctcgg gccgggccgc gtgaccgcct
     1201 gccccgtcac gggtgcggat gcgttgccgg aggtgtcgac cgcgaccacc tcgacgtcgt
     1261 aaggggtctg cggcgcaagg ccgtcgagcg tcagcgtgtg cccactctct tcccgaatga
     1321 tgtcgccgac ggtcacgacg tagcggtcca cgtcgacgtt gtcggaggcg ggggaccagg
     1381 tcagctggag ggtggaggag ccggcccgca cgctgaggtt cgtcggtgga gtcggaggcg
     1441 tgccatcggg cgtggcgccc gcggggaggg tggggaaggc cacgatggtg acaggtgaca
     1501 cattgccaga ggcgtcgcgc gccgtcacgc gcagctgatg gtcgcgcgcc ggcagcaggc
     1561 cggtgacggt cagggtgcct ggttcggtga tcgacagact ctgtccggcg acggtgacga
     1621 cgtatcgcac gacccccaca tcgtctcgcg cctcagcgac ggtgacagcc gcgcgggtgg
     1681 acccggggac gacgctgacc tcgggggagc tcggcgcctg cgtgtcgggt tcgagcagcg
     1741 tgcggacgtt gatccgaccc gcagtcgacc ggttgccggc tgcgtcgacg gcgaccacgg
     1801 cgacggccgt cgtcgtgccg accgggagat cggtcacggt ggccgaggtt ccgctgacct
     1861 cctgggtccg cccggccgcg gtgacgaggt agccggtgac ggcgacatcg tcggtggctg
     1921 cggtccaggt cactaccccc gtggtggtgc tctggccggt gacggcgagt gcggtcggct
     1981 cgctcggggc ggtggtgtcc ggcggcggga gggtggtgaa cgtggcctcc gcggcggctg
     2041 accggttgcc ggcggcgtcg gtggcgacga tggtggcggt gtatgtggtg tcaggcgtca
     2101 ggccgtcgag ggtcgccgtg gtggcggtga cggtctgctc ggcgagcccg gcgaccgcga
     2161 cgacgtatcc ggacacgccg acgttgtcgg tcgagggcgt ccagttcagc gtcgcctgtg
     2221 tgctcccggg tgtgaccgtg ggcgagccgg ggacgctagg cggggtgacg tccggttcgg
     2281 gcagggtgcg gaaggtggcg ctcaccgcgg gcgaggtgtt gccggccgcg tccttggcga
     2341 ggacgctggc cagatagctc gtgttcggcc gcaggcccgt gacggtggcc gtgcgggcgg
     2401 tgagggtctg ggatcgcccg tcaacggtca gcacgtaggc cgtcacgccg atgttgtcgc
     2461 tcgcggcacc ccaactgatg gtggcgccgg tgatctccgg ggcggtggtg atggcgcccg
     2521 gggcgctcgg gggcgtggtg tcccgggcct tggtgcgaaa cgccagcctg accgcggtgg
     2581 agcgattccc ggctgcgtcg acggcctgga cccgcgctgt atagctcgtg ttgggccgca
     2641 agttggcgaa tgccgccttg ggagcagtgg gccgtttggt ctgggtggcc accgtgacga
     2701 cgtagaactt gaccccgacg ttgtcggtcg aggccttcca ggtgagggtg acaccggtgg
     2761 tcgtcgagac cgcagtcaga ccgctcgggg cggtgggcgg cctggtgtcg cgggcggcga
     2821 ggctctggcc ggcggccagc aggctggcga ggacgaggac gaggggcagc agccagcggg
     2881 cacgcgggtg acggcgcatg acggtccttc atggggccga ggtgagactg gggggttaac
     2941 gttcaataat acccgcgagt cacgtctttg acagccccga ccgtgacgcc gtgcggagtg
     3001 gggccgagca ggccggcgcg gtggtgatta gggtgacccg tgtgagcgac gagcggcgcg
     3061 aggtggcggt gggcgagtgg atcgccgcca ccagggccgc gcgcgaggaa ggcttcgact
     3121 tcttcgactg gctgagcgcc gtcgatcgca cgagcgagga gccggccgga ttcgatgtcg
     3181 tggtgcacct gtatgacgtg tccacccccg gtgccctccg tggactgctc gtgcagaccc
     3241 ggatcgtcga gggcgaggcg ttgccgagtc tgaccggcgt gtttgccggc gcggcctggc
     3301 acgagcggga gacccacgag atgttcggca tcgacgtcgc cggcttcgac gacggctccg
     3361 gcctcggcct gcggcccctg ctgctgcccg acggcttcga gggtcacccg ctgcgcaagg
     3421 acttctatct cgcggcccgg gtgagcaagg actggcccgg cgcgaaagag ccgggccaac
     3481 cgcataccgg cgggcctggc cggcgcaaga tgcagccgcc cggagtgcca ccgcaggagt
     3541 ggggtccgcg atgagcatcc tcgagatcgt gctgcgggcc gccgccgcgc tggccatgct
     3601 cctcgtgctg ccgctcgtgg tcggccagac ggagcacaag gtcatggcgc acatgcaggg
     3661 ccggctcggg ccgatgtatg ccggggccta ccacggctgg gcgcagctgg ttgccgacgg
     3721 ggccaagttc gtgcagaagg aagacctggt tccacgggcg gcagaccggg gtgtcttcaa
     3781 gctcgctccc gcgctcgcga tgatccccta tctcctgggg ctggccgcca tcccgatcca
     3841 ccccgggtat gccgcgatcg aggcgcctgc cagcgtgctg ttcgtcctcg cggccacgag
     3901 catcggagtg ctcggcacct tgatggcggg ctgggcgagc gcgaacaagt acgccttgct
     3961 cggtgcgatg cgcggtgcgg cacaactgat ctcgtatgag ctgccgatgg tgctggctgt
     4021 cgcctcggtg gccctggccg cgggcacgct ctcgctcccc ggggtcatcg aggcgtggac
     4081 tccgtggtgg ctgctgtggc agctgccggg cgccttcgtc ttcctcgccg cggccgtcgc
     4141 cgaactgcag cgcccaccct tcgacatgcc gatcgccgat tcggagatcg tcttcgggcc
     4201 ctacaccgaa tacaccggcc tccggttcgc gctgttcctg ctcagcgagt atgccgggat
     4261 cgtcctgttc tccctgctct tcgccgtcct gtatctcggt ggct
//
LOCUS       DAOPJG010000237         4304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_22383_length_4304_cov_5.978819, whole genome shotgun sequence.
ACCESSION   DAOPJG010000237 DAOPJG010000000
VERSION     DAOPJG010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22383_length_4304_cov_5.978819"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKU75_15435"
     CDS             complement(<1..525)
                     /locus_tag="PKU75_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HPF82317.1"
                     /translation="MTAPAPRTEAVSARLSTLDRFLPVWILAAMALGLLLGRLLPSLG
                     DTLSAVEVDGISLPIAIGLLVMMYPVLAKVRYDRLDTVTGDRRLLVSSLVLNWLVGPA
                     LMFALAWLLLPDLPAYRTGLIIVGLARCIAMVIIWNDLACGDREAAAVLVALNSIFQV
                     IAFAALGWFYLSVLP"
     gene            complement(552..926)
                     /locus_tag="PKU75_15440"
     CDS             complement(552..926)
                     /locus_tag="PKU75_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPF82318.1"
                     /translation="MTSIEPTAAPETDICCAGAECDLLPTVEADVLADTFKALADPTR
                     VRLLRYLAESDAGTACACHLPDALGISQPTLSFHMRKLHDAGLVARDKRGRWVHWTVR
                     PEALAGIKTFLDLAPRSADRCC"
     gene            1021..1638
                     /locus_tag="PKU75_15445"
     CDS             1021..1638
                     /locus_tag="PKU75_15445"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPF82319.1"
                     /translation="MEPDRDTVTSQLVDDLTYSYQDIFEPAVIEKAVAEAWDLLAPAS
                     TIRTYLPILVARQAREQLMATAQAEGRVAKKVPEILFVCVHNAGRSQMAAALTEHLSA
                     RRVHVRSAGSAPTGDVLPVAIEALRERGIALGTAYPKPLSDSVVKAADIIVTMGCGDT
                     CPYFPGKRYEDWDVADPAGQPLEVVRDIRDDIQARVTALLREILD"
     gene            1666..2091
                     /locus_tag="PKU75_15450"
     CDS             1666..2091
                     /locus_tag="PKU75_15450"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPF82320.1"
                     /translation="MDTERPSVLFVCIHNAGRSQMGAAYTHHLSGGRVEVRSAGSAPA
                     DSINPAVREALLEDGIDISAEKPKILTTEAVEASDVVITMGCGDTCPYFPGKRYLDWT
                     LDDPAGQGVEAVRPIRDEIKARVRTLLGELGIDPVDDVV"
     gene            2202..>4304
                     /locus_tag="PKU75_15455"
     CDS             2202..>4304
                     /locus_tag="PKU75_15455"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPF82321.1"
                     /translation="MNEFQLSAPADFGPDEPSTNFRATGPQFPPTHHLADQPAVAPAR
                     DVDTFVQEFLEELNFGQGVPLDRSTINHQYLALASTVRSYLMADWLDTAARRRAHPAK
                     MVGYLSAEYLLGRQLGNALLNSDLEGIVEEAMRQLGIDLAAVRAQEVEPGLGNGGLGR
                     LAACFVDSLATMDVSCIGYGIRYEYGIFRQTFVEGRQVEVPDTWLALGAPWEFRNPER
                     AVQVDFGGSVESYADDQGRLRRRWRPDWNVLAVPYNYMVPGYHNGVANTLRLWSAEAT
                     RAFDLQIFNHGDYAEAVRAQTFAENISKVLYPEDSTPQGKELRLQQQYFFVAASIGDY
                     LHGTLPVDFDLARLPEQIIFQLNDTHPVIGIPELMRLLIDVHGMEWDRAWAITSRCFA
                     YTCHTLLPEALEVWPVSLVERLLPRHMEIIFEINDRFLHTVRERFPGDEDRVRRMSII
                     AEDGDRGVRMAHLATVAAVRVNGVAELHSQLLRDKVLVDFAQLWPEKFTNVTNGVTPR
                     RFLVSANPPLTSLINDAIGDGWITDLERLRELEPYAEDPVFRERFRSAKRANKDHLNS
                     TLLAAGTAQLPADHMPDVMVKRLHEYKRQMLKLLHIVSTCEGVLSGRVPADQVTPRTC
                     LFGAKAAPGYATAKETIYLINRVASVINSDPKLDGRLFVAFPPNYNVTLAQRLIPAAD
                     LSEQISLAGKEASGTGNMK"
BASE COUNT          762 a         1528 c         1353 g          661 t
ORIGIN      
        1 gggcaggacc gacaggtaga accagcccag cgcggcgaat gcgatgacct ggaagatcga
       61 gttgagggcg acgagcacgg ctgcggcctc gcggtcgccg caggccagat cgttccagat
      121 gatcaccatg gcgatgcatc gtgcgaggcc gacgatgatc aggccggtgc gataggccgg
      181 cagatccgga agcagcagcc acgcgagcgc gaacatgagg gccgggccga cgagccagtt
      241 caggaccagg ctcgacacga gaagtctgcg atcgcccgtc accgtgtcga gacggtcata
      301 gcggaccttg gccagcaccg ggtacatcat gacgaggagg ccgatggcga tgggcagcga
      361 gatcccgtcg acctccaccg ccgacagcgt gtcaccgagg gatggcagga ggcggcccag
      421 gaggagtcct agcgccatgg cggcaaggat ccacaccggc aggaaccgat ccagggtgga
      481 cagccgcgcg ctgacggcct cggttctcgg ggcgggcgcg gtcacggatt ctcctgtggg
      541 agacaacggg ctcaacaaca gcggtccgcg ctgcgcggcg cgaggtcgag gaaggtcttg
      601 atgccggcca gagcttccgg acggacggtc cagtgcaccc agcggccgcg cttgtcgcgc
      661 gcgacgagac ccgcatcgtg cagcttgcgc atgtggaagg acagcgtcgg ttggctgatc
      721 cccagcgcgt cgggaaggtg acaggcacac gccgtgccgg cgtcggactc ggcgagatag
      781 cgcagcagcc gcacgcgcgt gggatcggcg agggccttga aggtgtccgc caggacgtcg
      841 gcctcgaccg tgggcaacag gtcgcactct gccccggcac agcagatgtc cgtctccggt
      901 gccgcggtcg gctcgatcga ggtcatgacg caaccctagc gcacatattg acaatcatcc
      961 atatatggcc ttcaatcatg catagatgaa tgtcgatacg aagggacacg aggacacgcc
     1021 atggaacccg accgcgacac agtgaccagt caactggtcg acgacctgac ctactcctac
     1081 caggacatct tcgagcccgc cgtcatcgag aaggccgtcg ccgaagcctg ggacctgctc
     1141 gcgcccgcat cgaccatccg cacctacctg ccgatcctcg tcgcccgcca ggcacgtgaa
     1201 caactgatgg ccacggccca ggcggagggg cgggttgcca agaaggtgcc ggagatcctc
     1261 ttcgtgtgcg tccacaacgc cggacggtca cagatggcgg cagccttgac cgagcacttg
     1321 tccgcgcggc gcgtgcacgt gcgctcggcc ggctccgccc ccaccggcga cgtcctcccg
     1381 gtcgccatcg aggccctgcg cgagcgcggc atcgccctcg gcacggcata cccgaaaccg
     1441 ttgtccgaca gcgtggtcaa ggcggccgat atcatcgtga ccatgggctg cggcgacacc
     1501 tgcccctact tcccgggcaa gcgctacgag gactgggacg tggccgaccc cgccggtcag
     1561 ccactcgagg tggtccggga cattcgtgac gacatccaag cccgcgtgac cgccctcctc
     1621 cgcgagatcc tcgactgacc tcctccacga aaggcccgcc cacccatgga caccgagcgc
     1681 cccagcgttc tcttcgtctg catccacaac gccggccgct cccagatggg cgcggcatac
     1741 acccaccacc tctcgggcgg ccgggtcgag gtccgctcgg ccggctcggc gcctgccgac
     1801 tcgatcaacc ccgctgtgcg cgaagcgctc ctcgaggacg gcatcgacat ctccgccgag
     1861 aagcccaaga tcctgaccac cgaggccgtc gaggcctccg atgtcgtcat cacgatgggg
     1921 tgcggcgaca cctgccccta cttccccggc aagcgctacc tcgactggac cctcgatgac
     1981 cccgccgggc agggagtcga ggcggtccgt ccgatccgtg acgagatcaa ggctcgggtg
     2041 cgcaccctgc tcggcgagct cggaatcgac ccggtggacg acgtggtctg accggccggc
     2101 ccggaagttc gtccaggatt cttcgatcct gcctcgacgc tggcgcctcg aacctggaag
     2161 gttgggtact ggacccaccg ccttcgagag gacaagcgac cgtgaacgag ttccagctca
     2221 gcgcgccggc cgatttcgga cctgacgagc cgtcgacgaa cttccgcgcc acgggacccc
     2281 agtttccccc gacgcaccat ctagcggacc agccggcggt cgccccggcc cgcgacgtcg
     2341 acaccttcgt ccaggagttc ctcgaagaac tcaacttcgg tcagggcgtg ccgctggatc
     2401 gctccaccat caaccaccag tacctcgccc tggccagcac cgtccgcagc tacctgatgg
     2461 ccgactggct cgacaccgcg gcccgccgcc gcgcgcaccc ggccaaaatg gtcggctatc
     2521 tctcggccga atacctcctc ggacgccagc tcggtaacgc cctgctgaac agtgatttgg
     2581 agggcatcgt cgaagaggcc atgcggcagc tcggcatcga ccttgccgcg gtccgggccc
     2641 aggaagtcga acccggtctc ggcaatgggg gcctgggacg cctcgccgcc tgtttcgtcg
     2701 actcgctcgc caccatggac gtcagctgca tcggctacgg catccgctac gagtacggca
     2761 tcttccgcca gaccttcgtc gagggccgcc aggtcgaggt gcccgacacc tggctcgccc
     2821 tcggcgcccc ctgggagttc cgcaaccccg agcgggccgt gcaggtcgac ttcggcggca
     2881 gtgtcgagtc gtatgccgac gaccagggcc ggctgcggcg gcgctggcgc cccgattgga
     2941 acgtcctcgc cgtcccctac aactacatgg tccccggcta tcacaatggc gtcgccaaca
     3001 ccttgcgcct ctggagcgcg gaggcaactc gcgccttcga cctccagatc ttcaaccacg
     3061 gcgactatgc cgaggcggtc cgcgcccaga ccttcgcgga gaacatctcc aaggtgctct
     3121 accccgagga ctccaccccg cagggcaagg agttgcgcct gcagcagcag tacttcttcg
     3181 tggccgcctc gatcggtgac tacctgcacg gcaccctgcc ggtggacttc gatctcgccc
     3241 ggctgccgga gcagatcatc ttccagctca acgacaccca cccggtcatc ggcatacctg
     3301 agctgatgcg gctgttgatc gacgtccacg gtatggagtg ggaccgggcc tgggcgatca
     3361 cctcccgatg cttcgcctac acctgccaca ccttgcttcc cgaagccctc gaggtctggc
     3421 cggtcagcct ggtggagcgc ctgctgcccc ggcacatgga gatcatcttc gagatcaacg
     3481 atcgcttcct ccatacggtc cgggagcgct tccccggaga cgaggatcgt gtgcggcgga
     3541 tgtcgatcat cgccgaggac ggcgaccgcg gggtgcggat ggcgcatctg gccacggtcg
     3601 cggcggttcg cgtcaacggg gtcgcggaac tgcactcgca attgttgcgc gacaaggtgc
     3661 tggtcgactt tgcccagctg tggccggaga agttcacgaa tgtcaccaat ggcgtcaccc
     3721 cccgccggtt cctcgtgtcc gccaaccccc cgctcacgag cctgatcaac gacgcgatcg
     3781 gcgatggctg gatcaccgac ctggaacgcc tgcgcgagct ggagccgtat gccgaggacc
     3841 cggttttccg ggaacggttc cggtcggcga agcgcgccaa caaggaccat ctcaactcca
     3901 ccctgctggc cgcgggcacc gcgcagttgc ccgcggacca catgcccgat gtgatggtca
     3961 aacggctgca cgagtacaag cggcaaatgc tgaagctgct gcacatcgtc tcgacctgtg
     4021 aaggggtgct ctcgggtcga gttcccgcgg atcaggtcac gccccgcacc tgtctcttcg
     4081 gagccaaggc cgcgcccggc tatgcgaccg ccaaggagac gatctacctg atcaaccggg
     4141 tcgcgtcggt catcaactcc gatcccaaac tcgacggccg gctcttcgtt gccttcccgc
     4201 ccaactacaa cgtcaccctg gcgcagcggc tgatcccagc cgccgacctc tccgagcaga
     4261 tctcgctggc cggcaaggag gcctccggca ccggcaacat gaag
//
LOCUS       DAOPJG010000238         4249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_22808_length_4249_cov_4.703147, whole genome shotgun sequence.
ACCESSION   DAOPJG010000238 DAOPJG010000000
VERSION     DAOPJG010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22808_length_4249_cov_4.703147"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKU75_15460"
     CDS             <1..325
                     /locus_tag="PKU75_15460"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HPF82322.1"
                     /translation="NEDGTAGKFLWPGFGDNARVLKWVVERLDGTGEGVDTPVGIVPA
                     PGALDVSGLDLDDEDIAKALAVNPDEWETELALIQEWLAKFGDKLPTQMKDELATLKG
                     NLENA"
     gene            complement(398..1183)
                     /locus_tag="PKU75_15465"
     CDS             complement(398..1183)
                     /locus_tag="PKU75_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF82323.1"
                     /translation="MTVTVSPLTPLVAHTRAQVLDLIRTPIAIISTTIFPTLAFLFFV
                     LPQGAITDNAVASLSVVAQLGVFGVMSAYLFGYGIGVAEERANPWTTYLRTLPIGPFP
                     MTVARFLTAALASFLSLLPLVLAVALLTKAPEAFTSGELSWARIPFALAMVLIAATPF
                     LAMGLCIGYLLSSKAAVAVAQVLNFPLAFIGGLMLPPEMFPGWLDVVSLATPARAARD
                     IVIWALTGEQMPGSTLPVLLGWTVALTALAVWANRRDEGRRFR"
     gene            complement(1195..2169)
                     /locus_tag="PKU75_15470"
     CDS             complement(1195..2169)
                     /locus_tag="PKU75_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF82324.1"
                     /translation="MLHTQNQVRPTTDTITDTVADTVVASVESVSKSFGDTVALDDLS
                     MQVHAGESLGLLGPNGAGKSTLISLLTGTRRADTGTVTIFGGDPTSPDSRRRLGVTPQ
                     ATALPVTLRVREVFAFVGAHFGTTQEHLDELAASFDLADLLEKQCGSLSGGQQRRLMV
                     ALALVGDPELVILDEPTTGLDVESRDRLWARLRRYRDEGGTLVITSHYLAEIEALASR
                     VLVVDHGRVIADGTVRQITEQVQLRRILLRTTAPEQQLLALPGTVSVTPESESDPHHV
                     VLATRDADATVRALVADGIDFSDLQVHGASLEEAFLALTTHQSPAHAA"
     gene            complement(2231..2530)
                     /locus_tag="PKU75_15475"
     CDS             complement(2231..2530)
                     /locus_tag="PKU75_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPF82325.1"
                     /translation="MTDALPLDPVIHAEARLRVMTTLAALGPKDQMSFPRLQELLGMT
                     AGNLSTHNRKLEEAGYITVTKTIEGRSPATYLALTDTGRAAFGTYRKALAALLGG"
     gene            complement(2527..3222)
                     /locus_tag="PKU75_15480"
     CDS             complement(2527..3222)
                     /locus_tag="PKU75_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82326.1"
                     /translation="MNANDKPVVDPAADGSDAGFDPAEALRVMQDQDAKVDQQFGNPD
                     RKMYLVWGLAYLIGYLPLALSRGADPIVEIPIGIALAIFGLCIAAGIVAAVVLGVRAT
                     RGTRGESSRRGMLYGFSWWFSFMCIAGLSIQIGRSGADPEIAGLIVNGASMALVAALL
                     MAGGVIWLDVSQFVIGACVALITLIALVVGLPAYWWIMCLGAGGLLLLAALFHRPLRR
                     ILPDGALAQDAAP"
     gene            complement(3365..3604)
                     /locus_tag="PKU75_15485"
     CDS             complement(3365..3604)
                     /locus_tag="PKU75_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82327.1"
                     /translation="METSAAFGNNAGKCERAAYPWRGMPPGGEQGQLDPNGGQGQMDP
                     NGGTGQADPNGSSSSNGSTTPSTPKQGNTLPVPAT"
     gene            3692..4111
                     /locus_tag="PKU75_15490"
     CDS             3692..4111
                     /locus_tag="PKU75_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82328.1"
                     /translation="MWTPDDFPGADNVPWQLLIRARFAHEIDAVIASLIVNQVGAKLG
                     TKRAVQLARAGTVQHSKEAATSEQKVAVLGLLADWDGEICPKNWPFRWPPRKRGFEEL
                     DDPITVIGLGRGLELASLGSAELKDQLGSVLNEIGRG"
BASE COUNT          689 a         1280 c         1657 g          623 t
ORIGIN      
        1 caacgaggac ggcacggccg gcaagttcct gtggcccggc ttcggtgaca acgctcgcgt
       61 cctgaagtgg gtcgtcgagc gcctcgacgg caccggcgag ggcgtcgaca ccccggtcgg
      121 catcgtcccg gccccgggcg ccctcgacgt cagcggtctc gacctggacg acgaggacat
      181 cgccaaggcc ctggccgtca acccggacga gtgggagacc gagctcgcgc tcatccagga
      241 gtggctcgcc aagttcggcg acaagctccc gacccagatg aaggacgagc tggccaccct
      301 caagggcaac ctcgagaacg cctgacggtc gcacccaagg tgagcgggcg gcataccaaa
      361 tcattggtat gccgcccgct cacctttgcg ttgtgactca ccggaagcgg cgaccctcgt
      421 cgcgacggtt ggcccacacg gcgagtgcgg tcagcgcgac ggtccagccg aggaggacgg
      481 ggagggtcga tccgggcatc tgctcacccg tcagtgccca gatgacgatg tcgcgggcgg
      541 ctcgcgccgg ggtggccagg gagacgacat cgagccagcc ggggaacatc tccggtggga
      601 gcatgaggcc accgatgaag gcgagcggga agttgaggac ctgtgcgacg gcgacggccg
      661 ccttgctgct caggaggtag ccgatgcata ggcccatcgc gaggaagggc gtcgccgcga
      721 tgaggaccat cgcgagggcg aacgggatcc gggcccacga gagctcgccg gacgtgaagg
      781 cctccggtgc cttggtcagc agtgcgaccg ccagcacgag cggcagcagg ctgaggaagg
      841 acgcgagcgc ggcggtcagg aagcgagcga cggtcatcgg gaacgggccg atcggcaggg
      901 tgcgcaggta ggtcgtccac gggttggcgc gctcctcggc gacgccgatg ccgtagccga
      961 agaggtaggc ggacatgacg ccgaagacgc cgagttgggc gacgaccgag agggacgcca
     1021 ccgcgttgtc ggtgatcgcg ccctggggca ggacgaagaa gaggaaggcc agggtcggga
     1081 agatcgtcgt cgagatgatc gcgatcgggg tgcggatcag gtcgaggacc tgggcgcggg
     1141 tgtgcgcgac caggggggtg agggggctga cggtgacggt catgtcgagt cctttcaggc
     1201 cgcgtgggcc gggctctggt gggtggtcag ggccaggaag gcctcctcga gggaggctcc
     1261 gtggacctgg aggtcgctga agtcgatgcc gtcggcgacg agggcgcgga cggtggcgtc
     1321 ggcgtcgcgg gtggcgagga cgacgtggtg ggggtcggac tcggactcgg gggtgacgga
     1381 gaccgtgccg gggagggcga gcaactgctg ctcgggggcc gtcgtgcgca ggaggatccg
     1441 acggagctgg acctgctcgg tgatctgacg gacggtgccg tcggcgatga cccggccgtg
     1501 gtcgacgacg agcacgcggg aggcgagggc ctcgatctcg gcgaggtagt gactggtgat
     1561 gacgagagtg ccgccctcgt cgcgatagcg acgcagccgc gcccagaggc ggtcgcggga
     1621 ctcgacgtcg agccctgtcg tcggctcgtc gaggatgacg agctcagggt caccgacgag
     1681 ggcgagcgca accatgagcc gacgctgctg cccaccggac agtgagccgc actgcttctc
     1741 gagcaggtcg gccaggtcga aggacgcggc cagctcgtcg aggtgctcct gggtggtccc
     1801 gaagtgggcg ccgacaaagg cgaagacctc acggacccgg agcgtgacgg gcagggcggt
     1861 ggcctgcggg gtcacaccga ggcggcgacg ggagtcaggg ctggtcgggt cgccgccgaa
     1921 gatcgtgacg gtgcccgtgt ccgctcggcg ggtgccggtg agcaggctga tcagggtgga
     1981 cttgccggct ccgttcgggc cgagcagacc aaggctctcc ccggcatgga cctgcatcga
     2041 gaggtcgtcg agcgcgacgg tgtcgccgaa gctcttggag accgactcga cgctggcgac
     2101 gacggtgtcg gcgacggtgt cagtgatggt gtcagtggtg ggacggacct gattttgggt
     2161 gtgcagcatc gcgattcctc catcgagagg ggtttgaggg gtctggtgtg gcgggtcggt
     2221 attggggtgc tcagccgccg aggagggcag ccagagcctt gcgataggtg ccgaacgcgg
     2281 cacggccggt gtcggtgagg gcgaggtagg tcgcgggcga gcggccctcg atggtcttgg
     2341 tgaccgtgat gtaacccgcc tcctcgagct tgcggttgtg ggtggagagg ttgccggcgg
     2401 tcatgccgag caactcctgg aggcggggga aggacatctg gtccttgggt ccgagcgccg
     2461 cgagcgtggt catgacgcgc agtctggcct cggcatggat gaccgggtcg agcgggaggg
     2521 cgtcggtcat ggtgccgcgt cctgggccag cgcgccgtcg gggaggatgc ggcgcaacgg
     2581 gcgatggaag agcgcggcga gcaggagcag gccaccggcg ccgaggcaca tgatccacca
     2641 gtaggccggc aggccgacga cgagtgcgat gagggtgatc agggctacgc acgccccgat
     2701 gacgaactgc gacacgtcga gccagatgac accgccggcc atgagcaggg ccgcgacgag
     2761 ggccatggag gcgccgttga cgatgaggcc ggcgatctcg gggtcggcgc cggacctgcc
     2821 gatctggatg gagaggccgg cgatgcacat gaacgagaac caccaggaga agccgtagag
     2881 catgccgcgg cggctggact cgccgcgcgt gcctcgggtg gcgcggacac cgaggacgac
     2941 agcggcgacg ataccggcgg cgatgcagag gccgaagatg gccagggcga tgccgatcgg
     3001 gatctcgacg atggggtcgg cgccacggga gagggcgagc ggcaggtagc cgatcaggta
     3061 ggccaggccc cagacgaggt acatcttgcg gtcggggttg ccgaactgct ggtcgacctt
     3121 ggcgtcctgg tcctgcatca cccgcagcgc ctcggccggg tcgaagccgg cgtcgctgcc
     3181 gtcggcagcg gggtcgacga cgggcttgtc gtttgcgttc ataagtactt tgtactgcaa
     3241 accactttgc gtgtcaagag acaggtgggt tcccggtcga ctgcggacgt gacggtgccc
     3301 ttggacgcgt cgacgcgcgt ccaagggcaa agtccgagag aggccagggg ggaagagggt
     3361 gagatcaggt cgccgggacg gggagggtgt tgccctgctt cggggtgctg ggcgtggtcg
     3421 aaccgttcga gctgctcgag ccgttcgggt ccgcctggcc ggtgccgccg ttgggatcca
     3481 tctgtccctg gccaccgttc gggtcgagtt gaccctgctc gccgccgggc ggcatccccc
     3541 tccatgggta tgccgcccgc tcgcatttgc cggcgttgtt cccgaatgcc gcgcttgttt
     3601 ccatggccgc cggtgtatca cggcaccccg atcgccctcc tccttggcag accgccggac
     3661 cgcccggtgg cacgcaccca aggagttcct catgtggaca cccgatgact tccccggcgc
     3721 cgacaacgtt ccgtggcagc tgctcatccg tgcccggttc gcgcacgaga tcgatgccgt
     3781 catcgcctcg ctcatcgtca accaggtcgg cgccaagctc ggcacgaagc gcgccgtgca
     3841 gctggcccga gctggcaccg tccagcacag caaggaggcc gccacgagcg agcagaaggt
     3901 cgccgtgctc ggcctgctgg ccgactggga cggagagatc tgcccgaaga actggccctt
     3961 ccgctggcct ccgcggaagc ggggcttcga ggagctggac gacccgatca ccgtcatcgg
     4021 tctgggccgt ggactcgagc tcgcctcgct cggctccgcg gaactcaagg accaactggg
     4081 ctcggtcctg aatgagatcg gtcgcggctg accaacccgg acaccctggg taacgcccga
     4141 gggcccatgg agatccatgg gcccccgggc gttgctgggt gacgggcgaa ctcagccggc
     4201 ggtgccgggc aggctcgggc cctgttgctg ggtgttgcca ttggagttg
//
LOCUS       DAOPJG010000239         4222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_23013_length_4222_cov_6.347732, whole genome shotgun sequence.
ACCESSION   DAOPJG010000239 DAOPJG010000000
VERSION     DAOPJG010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23013_length_4222_cov_6.347732"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PKU75_15495"
     CDS             <1..988
                     /locus_tag="PKU75_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF82329.1"
                     /translation="GRAIDKALDASIAGGFGEPGYLLRRHLPAWPDDPRPNALVGKHI
                     AVTGASSGIGVAASRQLSDLGAHVHLIVRNPDKATPVAAALPGASTVWMCDLADLDSV
                     ATCAKELDEKGIDLSGLVHNAGVMPARHTLTPQGFEQSMAVHVLGPALLTELLRRRLS
                     GGRVVLVTSGGMYAQRLRIDDLAYREEPYRPAKAYARSKRAQVELLQPLQDRWSDLTV
                     AAMHPGWVDTPGVRESLPGFARLTHPVLRDADAGADTITWLAATETRLPGGRLWHDRQ
                     ARPSNRLGRTAAGHDERDVLLDWVYEALEPWLEPGRNPHPGREEGGLAREVE"
     gene            complement(1034..2218)
                     /locus_tag="PKU75_15500"
     CDS             complement(1034..2218)
                     /locus_tag="PKU75_15500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HPF82330.1"
                     /translation="MKALTWQGMRSVEVREVPDPVIVDPTDAIVRVTSTAICGSDLHL
                     YNVLAPYLQPGDILGHEFMGVVEEVGSAVANIAVGDRVVVPFNISCGHCWMCDRGLFA
                     QCETTQNRDQGKGGSLFGYTALYGSVPGGQAERVRVPHADFGPVKVPDGPADERFLFL
                     SDILPTAWQGVTASGVQPGETLAVLGAGPVGLLAVSAAQHLGIDKVISVDLVPERLAA
                     AAGRGARTVDMGSVTSVGDALRDLTDGRGPDAVVDAVGMEAHGNPVAGALISATARVP
                     KPLARKAIETVGIDRLNALHTAIDAVRRGGCVSLSGVYGGSADPMPLMDMFDKAITMR
                     MGQCNVRHWTDRLLEILCQEDDVLGVEALTTHRAGLDEAAEMYATFDARTDGCIKVVM
                     KP"
     gene            complement(2215..3231)
                     /locus_tag="PKU75_15505"
     CDS             complement(2215..3231)
                     /locus_tag="PKU75_15505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF82331.1"
                     /translation="MTHRIPERRPAAPGLALVVGASRGLGMAIAADLTQRGHPVVICA
                     RTGDDLETAQAQLTARRPGATVHTRVVDIGDEHAVDALVADVEETLGPIDIAIAVAGT
                     IQVGPAETMTTEEFRDAMGTMAWGPIHLAWSVLPAMRRRGHGRIGVITSIGGMVSPPH
                     LLPYATAKFAAVGFSDGLAAALSGTGVTATTVVPGLMRTGSHRRAQFAGDAAAEYAWF
                     APAASLPLLSMDVERAAARIVGGVLRGRPMVILTPLAKVGVRVRGLMPATTTRVMGLA
                     NRLLPESDRAPRELGTIRPRLVEGGEADRRLGSPLVRRLTVLGERAADTWNERRGERK
                     EQLS"
     gene            complement(3716..>4222)
                     /locus_tag="PKU75_15510"
     CDS             complement(3716..>4222)
                     /locus_tag="PKU75_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPF82332.1"
                     /translation="GKRMSTDAITVLKADHKEVARLFAEFEKKATTPARKEQIVRKVI
                     ELLTVHTYIENEILYPEARERVPDLEQHILESYEEHHVVDLLATELDAMTREDERFEA
                     KFTVLIENVRHHVKEEEDEWFPKLRDALGRSQLSEMGARMLELKKTAPTGPSQPSALK
                     KAIDAVVS"
BASE COUNT          675 a         1419 c         1445 g          683 t
ORIGIN      
        1 cggacgcgcc atcgacaagg cgctcgatgc gagcatcgcc gggggattcg gcgagcccgg
       61 ctatctcctc cgccggcatc tgccggcgtg gcccgacgac ccgcgtccga atgccttggt
      121 gggcaagcac atcgcggtca cgggtgcctc gtccggcatc ggcgtggccg cctctcggca
      181 gctttcggac ctaggtgccc acgtccatct catcgtgcgc aatcctgaca aggcgacccc
      241 tgtcgccgct gctctgccag gcgcaagcac cgtatggatg tgcgatctgg ccgatctcga
      301 ctcggtcgcc acgtgtgcga aagagttgga cgagaaggga atcgatctct cggggctcgt
      361 gcacaacgca ggggtgatgc cggcgcggca caccctgaca ccgcaagggt tcgagcagtc
      421 gatggccgtc cacgtgctcg gaccggcgct gctgacggaa ctgctgcggc gtcgcctcag
      481 cggaggccgg gtcgtgttgg tgacctcggg cggcatgtac gcgcagcgcc tgcgtatcga
      541 tgatctcgcc taccgcgagg agccctatcg gccggccaag gcttatgcgc gcagcaagcg
      601 ggctcaggtg gaactgctcc agccgctcca ggacaggtgg tccgatctga cggtggccgc
      661 catgcacccg gggtgggtgg acactcccgg ggtgcgcgaa tccctgcccg gattcgcgcg
      721 gctgacccat cccgtcctgc gcgacgcgga cgcaggggcg gataccatca cgtggctcgc
      781 ggccaccgaa acccggctgc ccggtggccg gctttggcat gaccgccagg cgcgtccgag
      841 caaccgactg ggccgtaccg ctgcgggcca tgacgagcga gacgtccttc tcgactgggt
      901 ctatgaggcc ctcgagccgt ggttggagcc gggtcgaaac ccgcatccgg gccgggagga
      961 aggtggactc gcccgtgaag tcgaatgacg gcaattgcac accgatcgac acgtcgtgag
     1021 ctgaacctgc cggctacggc ttcatgacga ccttgatgca accgtccgtg cgcgcatcga
     1081 aggtggcgta catctccgcg gcctcgtcga gcccggcgcg atgggtggtg agcgcctcca
     1141 caccgagcac gtcgtcctct tgacacagga tctcgagcaa ccggtcggtc cagtggcgga
     1201 cattgcactg gcccatgcgc atggtgatcg ccttgtcgaa catgtccatc agcggcatgg
     1261 ggtccgcact gccgccgtag acgccgctca gcgagacgca gccgccgcgc cgcaccgcgt
     1321 cgatggccgt atgcagcgca ttgagccggt cgatgcctac ggtttcgatt gccttgcgcg
     1381 ccaatggctt cgggactcgt gccgttgcgg agatcagggc gcccgcgacc gggttgccgt
     1441 gggcctccat gccgacagcg tcgacgacgg cgtcggggcc gcgcccgtca gtcagatccc
     1501 gcagggcgtc gccgacggag gtgaccgagc ccatgtcgac cgtgcgcgca ccgcggccgg
     1561 cggcagccgc aagtcgctcc gggaccaggt ccacgctgat caccttatcg atgccgagat
     1621 gttgtgcggc ggacacggcc agcaggccga ccggaccggc gcccagcacc gccaaggtct
     1681 caccgggctg caccccggag gcggtcaccc cctgccaggc cgtcgggagg atgtcggaca
     1741 ggaagaggaa ccgttcgtcg gccggtccgt ccggaacctt gaccggtccg aagtcggcgt
     1801 gcgggacgcg cacccgctcg gcctgaccgc cgggcaccga gccgtacaac gcggtgtagc
     1861 cgaacaggct cccgcccttg ccttggtctc ggttctgggt cgtctcgcac tgggcgaaga
     1921 ggccgcgatc gcacatccag cagtgtccgc aggagatatt gaaggggacg acgacccggt
     1981 ctcccacagc gatattggcg accgcgctgc cgacctcctc gacgacgccc atgaactcat
     2041 gaccgaggat gtcgcccggc tggagatacg gggcgagcac gttgtacagg tgcaggtcgg
     2101 aaccgcagat cgcggtcgag gtcacgcgca cgatcgcgtc cgtcgggtcg acgatgaccg
     2161 gatccgggac ctcccggacc tcgacggagc gcattccttg ccaggtgagg gccttcatga
     2221 cagttgttcc tttcgttcgc cgcggcgttc gttccaggtg tcggcggccc gctcgcccag
     2281 cacggtcaag cggcgcacga gtggcgagcc gagccgccgg tccgcctcgc cgccctcgac
     2341 caggcgcggg cggattgttc cgagttctcg gggggcgcgg tcggactccg gcagcagccg
     2401 gtttgccaga cccatgacgc gcgtcgtcgt tgccggcatc aatccgcgca cgcgcactcc
     2461 gaccttggcc aagggcgtca ggatgaccat cggccggccg cgcagaacgc cgccgacgat
     2521 gcgcgccgcc gcccgctcca cgtccatact caagagcggc agcgatgcgg ccggggcgaa
     2581 ccacgcgtac tcagcggccg catcgccggc gaactgggcc cgccggtgag agccggtccg
     2641 catcaggccg ggcacgaccg tcgtcgccgt cacacctgtc cccgacaggg ctgccgccaa
     2701 cccgtcggag aagccgaccg ccgcgaattt cgctgtggca taaggcagta gatggggcgg
     2761 cgagaccatg ccaccgatcg aggtgatgac gccgatacgg ccatgaccgc ggcggcgcat
     2821 ggcgggcagg accgaccacg cgagatggat cgggccccag gccatggtgc ccatggcgtc
     2881 ccggaactcc tccgtcgtca tcgtctctgc gggcccgacc tggatggtgc cggccacggc
     2941 aatcgcgatg tcgatggggc cgagggtctc ttcgacgtcc gcgacgagtg catcgaccgc
     3001 gtgttcgtcg ccgatgtcga ccacgcgcgt gtgcacggtc gctccgggcc gccgcgccgt
     3061 cagctgggcc tgcgcggtct cgaggtcatc gccggtccgg gcgcagatga ccaccgggtg
     3121 gccgcgctgc gtgaggtcgg cggcgatggc catgcccaag ccccgcgacg cgccgacgac
     3181 cagggcgagc cctggggcag ccggccggcg ttcgggtatg cggtgcgtca tctcattcct
     3241 tcctgcggtt ggcgcttcct gtggtttgcg caatcgcgcc gggccacgac catatgcacg
     3301 gcgggcggca ccgcgataca ccctccggag tggccggggg aaatggcggt cgacctaggg
     3361 ctgggtggct cctccgatcc cccttgctca cgggggcgaa ggaacgccaa ctcgcgacac
     3421 cgccaaagaa gcgctcgagg aggagaagtc ccacccctca tcaacgaagt gaatgaagtt
     3481 ggtcaggaga ccgaactaga cctgaagaaa ccggcgaaag aagcggcctc ggcgttgatg
     3541 gcttcggccc ggggacgcgt ggagagagtt gacgctcgag ggccatgacg cagccacgac
     3601 gtgacgacac cgcccagacc tcccgagccg cgatcaaaaa gagacacgca gcgactcgtc
     3661 ctgaacaacg ctgggtgggc tgcgggcccc tttgactgtg cagccaccga cgctcctatg
     3721 acacgacggc gtcaatggcc ttcttcaacg cggacggttg cgatggccca gttggagcgg
     3781 tctttttcag ctcgagcatc cgggcgccca tttcggatag ctgggagcgg ccgagcgcgt
     3841 cccgcagctt cgggaaccac tcgtcctcct cttccttgac atggtggcgc acgttttcga
     3901 tcaagacggt gaacttggcc tcgaagcgtt cgtcctcgcg cgtcatcgca tccagctcgg
     3961 tcgcgagcag gtcgacgacg tgatgctctt cgtaggactc caggatgtgc tgctcgaggt
     4021 cgggaacccg ttcgcgagct tcgggataga ggatttcgtt ctcgatgtag gtgtgcaccg
     4081 tcaggagctc gatgaccttg cggacgatct gttccttgcg cgcgggggtg gtggctttct
     4141 tctcgaattc tgcgaacagg cgggcgactt ccttgtggtc ggccttcaag acggtgatgg
     4201 cgtcggtgga catccgtttt cc
//
LOCUS       DAOPJG010000240         4201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_23182_length_4201_cov_7.102267, whole genome shotgun sequence.
ACCESSION   DAOPJG010000240 DAOPJG010000000
VERSION     DAOPJG010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23182_length_4201_cov_7.102267"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PKU75_15515"
     CDS             complement(<1..859)
                     /locus_tag="PKU75_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPF82333.1"
                     /translation="MTTAPAQHPALPERPTLDGLEEKWGAVWQQQGTYAFDRERALTL
                     PREQIFAIDTPPPTASGSLHMGHVFSYTHTDCMARYKRMLGHEVFYPIGWDDNGLPTE
                     KRVQNYYGVRGDATLPYVENFEPPHRGTATSTKSAHEVPISRANFLELCEERTVEDEK
                     SFESLFRRLGLSFDWQQTYRTIDDFSRATCQWAFIRNVQRGEAYQSEAPGLWDVTFQT
                     AVAQAELEARDYPGHYHRVAFHAEDGPVYIETTRPELIPSVVALIAHPDDERYAARFG
                     TTVTSPLFGV"
     gene            1156..1989
                     /locus_tag="PKU75_15520"
     CDS             1156..1989
                     /locus_tag="PKU75_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPF82334.1"
                     /translation="MHPHPIGGGSFPSPVVPGTGWPGDPATASTSVARDAGSVEMLAG
                     QCDSLAELAAEVSVCRACPRLVAWREQVAREKRAAYADQPYWGRPAPSFGDPNARILI
                     VGLAPAAHGANRTGRMFTGDRSGDWLYAALHRAEFASQPASTAAGDGMVLRGIRITAA
                     VHCAPPENKPTTDEKRSCAPWLDRELLLSPGVRVVIALGGVGWDATLAAARRLGWEVP
                     RPKPSFGHANSTIVRRPDGSEVLLLGSYHVSQQNTFTGRLTEPMLDAVVMAARRRASG
                     V"
     gene            2267..>4201
                     /locus_tag="PKU75_15525"
     CDS             2267..>4201
                     /locus_tag="PKU75_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82335.1"
                     /translation="MDAAYDSWVADGGLAREAQADHQRLDVEADSPEWHAADEEAWLA
                     ALDARISAGDYDEWLRVELAAPAGDATPGADSGVTPTPASAPGRVGFEGLTVSTVTVQ
                     AFVDWLASVIPHPDTVGDAAVVPADRPAGPSAAPAAPAASELVDLLAMLSRLPGAIAA
                     AQGRVSLELGRTTIRDAVAAGTSAVKAREAVAPQVGLARKCSPARAEQHLALARALDG
                     MPNTHRALTAGLIEIAHAAAVASATNCLSEDHRAEVDALLADRLVGITAKRLGQIAAK
                     YAAELDPQAVVAKHKKARSERAVWTRPAPDGMTYLTIFGPLTDVISALAALRRHAKTV
                     ANGGLDEEDPAEGRTESQIMADAALRWLAGRDRHQAQPVALNLVMHPDSLFGPNGHYT
                     STPCPSGSFAATRTKSGTANAPYAPASARSSTAPHAEPTDAEPTVPERGDTEPGDTEP
                     GDGEVNVPPAGSRPRTAPGETDPTTGAAFGSAHTDLDPSDRPGPIIWLGGGARTGRVN
                     TPAWLVGHGPMPAPLARALLLHGCHPEGQNPDCQDPDCQDPGSPHLGGSTGFDPASRP
                     GHTVDPSGSATPNRAAQVTFRRIFTSPDGRDLVALESTARLFPAGVRRALILRDDHCR
                     TPYCDADIRHADHVHPAARGG"
BASE COUNT          623 a         1448 c         1478 g          652 t
ORIGIN      
        1 cgaccccgaa caacggcgag gtgaccgtgg tgccgaagcg cgcggcatac cgctcgtcgt
       61 cgggatgggc gatcagggcc accaccgacg ggatcagctc ggggcgcgtc gtctcgatgt
      121 agacggggcc gtcctcggcg tggaaggcga cccggtggta gtggccgggg tagtcgcgcg
      181 cctccaactc ggcctgcgcc accgcggtct ggaaggtgac atcccacaac cccggcgcct
      241 cggactggta ggcctcgccg cgctggacat tgcggatgaa cgcccactgg caggtcgccc
      301 gggagaagtc gtcgatggtg cgataggtct gctgccagtc gaaggagagg ccgaggcggc
      361 ggaagagcga ttcgaagctc ttctcgtcct cgacggtgcg ctcctcgcac agctcgagga
      421 agttggcgcg gctgatcggc acttcgtggg ccgacttggt cgatgtggcc gtgccgcggt
      481 ggggcggctc gaagttctcg acatagggca gggtcgcgtc accgcgcacg ccgtagtagt
      541 tctgcacgcg cttctcggtg ggcaggccgt tgtcgtccca gccgatcgga tagaacacct
      601 cgtgccccag catccgcttg taccgcgcca tgcagtcggt gtgcgtgtag ctgaagacgt
      661 gccccatgtg caggctcccg ctcgcggtcg gcgggggagt gtcgatggcg aagatctgct
      721 cccgcggcag ggtgagcgca cgctcccggt cgaaggcgta ggtgccctgc tgctgccata
      781 ccgcgcccca cttctcctcc aggccgtcga gcgtgggccg ctcggggagg gcagggtgct
      841 gcgcgggtgc ggtggtcatg gttgggaatt ctttcatccc gtatgccgtg gtctcgaccg
      901 ggtttcgcgc cggtgtcccg cgcttgggcg atctataggt ggcgcgcttt gctcgcgatc
      961 ctggtggcga tccccggcag tgggacgtcg ggcgaggccg aaagatgcgc ctcgagaact
     1021 gctaggcaat cccacccccg tagcgtctgc gtcaagaggg gtatgcggcc cgccaccagc
     1081 cctggcctgg aatgtcatgc gcggtgcgcg cgaggagacc gcggcggcgc ataggacgat
     1141 agcggcgtgg cctctatgca tccgcacccg atcggcggcg gttcctttcc ctcgccagtg
     1201 gtccctggga cagggtggcc gggggatccg gccaccgcgt cgacatcggt ggcgcgggat
     1261 gccgggtcgg tggaaatgct tgccggtcaa tgcgattcgc tcgccgagtt ggcggccgag
     1321 gtttcggtat gccgcgcctg tccccggctg gttgcgtggc gggagcaggt ggcacgggag
     1381 aagcgggcgg cgtatgcgga ccaaccctat tggggtcggc cggcgccctc cttcggtgac
     1441 ccgaacgctc gtatcctcat cgtcgggctg gctcccgccg cccacggcgc caaccgcact
     1501 gggcggatgt tcaccggcga ccggtcgggg gattggctgt atgccgcgct gcaccgcgcg
     1561 gagttcgcca gccagcccgc gagcactgcc gcgggtgacg gaatggtgtt gcgcggcata
     1621 cggatcacgg cggcggtgca ctgcgctccg ccggagaaca agccgaccac cgacgagaaa
     1681 cgcagttgtg cgccatggct cgatcgggaa ctccttctct cgcccggcgt tcgggtggtg
     1741 atcgctctgg gtggcgtcgg atgggatgcc acgctggccg ccgcccgtcg gctgggctgg
     1801 gaagtgccgc ggccgaaacc gagtttcgga cacgcgaatt cgaccatcgt ccggcgaccc
     1861 gacgggtccg aggtgttgct cctcggctcc tatcacgtca gtcagcagaa caccttcacc
     1921 gggcggctca ccgaaccgat gctggacgcc gtcgtgatgg cggcccggcg ccgcgcatcg
     1981 ggggtttaaa gggcggttgt cggtgggctc tggcagggtc tggtgatcgg gcagtttgcg
     2041 gttcgttatc gagttgctga ctggtcgttg cggtggcgct tgcggaagct aatcacattg
     2101 cgctgcagcg aaattcatca gaataggcct tgcatgactg atagaactgg agtacgataa
     2161 gaccatgacg accacggcac acgatccccg acctggagct tcctgctccc ttggtcgcgc
     2221 gtgccctgac gttgtccctg acttgccgcc gctggagcct gcgcaggtgg acgcggccta
     2281 cgactcctgg gtcgccgacg gcggtctggc ccgcgaagcg caggcggatc accagcgcct
     2341 cgatgtggag gcggacagtc ccgagtggca cgctgccgac gaggaggcct ggctggcagc
     2401 gctcgatgcc cggatcagcg cgggggacta cgacgagtgg ctccgcgtgg aactggccgc
     2461 cccggcgggc gatgccacgc ccggagcgga ctcaggggtg acacccacgc cggcctccgc
     2521 gccaggccgt gtgggcttcg agggtttgac ggtctccacc gtcaccgtgc aggcattcgt
     2581 ggattggctg gcgtcggtga tcccgcatcc ggacactgtt ggcgacgctg ccgtcgtccc
     2641 cgccgatcgg ccggccgggc cgtccgcggc accggcggca ccggcggcga gcgagctcgt
     2701 ggatctgctg gcgatgctct cgcggctgcc gggagcgatc gctgccgctc agggccgggt
     2761 gtcgctggag ttggggcgca ccacgatccg cgacgccgtc gcggcgggca cgtccgcagt
     2821 caaggcccgc gaggcggtgg cgccgcaggt cgggctggcc cgcaagtgct cacccgcgcg
     2881 ggcggagcag cacctggcgt tggcacgggc gttggacggg atgcccaaca cgcatcgcgc
     2941 gttgacggcc gggctgatcg agatcgcgca cgcagccgcc gtcgcgagcg ccaccaactg
     3001 tctcagtgag gatcatcgag ccgaggtcga cgcgctgctc gctgatcggc tcgtggggat
     3061 cacggcgaaa cgcttgggac agatcgccgc caagtacgcc gcggaactgg acccccaagc
     3121 ggtggtggcc aagcacaaga aggcccgctc cgagcgtgcc gtctggacgc ggcccgcccc
     3181 ggatgggatg acctacctga ccatcttcgg acccttgacc gatgtcatct ccgcactcgc
     3241 cgcattgcgc cggcatgcca agaccgttgc caacggcggc ctcgacgagg aggacccggc
     3301 cgaggggcgc accgaaagcc agatcatggc cgacgcagcg ctgcggtggc tcgccggacg
     3361 cgaccgccac caagcccaac cggtcgccct caacttggtc atgcaccccg acagcctctt
     3421 cggccccaac ggtcactaca ccagcacacc gtgcccgtcc ggatcgttcg cagccacccg
     3481 cacaaagtcc gggacggcca acgcgccgta cgcgccggca tcggctcgga gttcgacggc
     3541 cccacacgcc gagccgaccg acgccgagcc cacggtccct gagcgtggcg atactgagcc
     3601 aggtgatacc gagcctggcg acggcgaggt caacgtgccc cctgcgggtt cacgtccgcg
     3661 gacagcacca ggcgaaaccg acccgacaac gggagccgct ttcgggtccg cgcacaccga
     3721 tcttgatccg tccgatcgac ccggcccgat catctggcta ggcgggggcg cccggaccgg
     3781 gcgagtcaac acgcctgcct ggctggtcgg ccacggcccg atgccggcgc ccctggcccg
     3841 cgcactcctg ctgcacggct gccatcccga gggccagaac cccgactgcc aggaccccga
     3901 ctgccaggac cccgggagcc cccacctcgg cggctcgacc ggcttcgatc ctgcaagtcg
     3961 acccggccat accgtcgacc ccagcggttc ggcgaccccg aatcgggctg cccaggtcac
     4021 cttccgacgc atcttcacca gcccggacgg ccgcgacctg gtcgcgctcg agtccaccgc
     4081 gcgcctgttc ccggccggcg tgcgccgcgc cctgatcctg cgcgacgacc actgccgcac
     4141 gccatactgc gacgccgaca tcagacacgc cgaccacgtg cacccggcag cccgcggcgg
     4201 a
//
LOCUS       DAOPJG010000241         4171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_23435_length_4171_cov_7.212342, whole genome shotgun sequence.
ACCESSION   DAOPJG010000241 DAOPJG010000000
VERSION     DAOPJG010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23435_length_4171_cov_7.212342"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(740..1282)
                     /locus_tag="PKU75_15530"
     CDS             complement(740..1282)
                     /locus_tag="PKU75_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82336.1"
                     /translation="MFTDFIRDHAFTIAWFGLMAFVWFGWGQEDPPPAWRSWLGAGSV
                     MGLLLAGLFGYGVFARWSEGSALEGRYPWFGLVVGLEVLLAVVGCLFLHRRGKGRWMA
                     WWVALVVALHFVPLAFFLDDLSLAGLGIIEAAGLAALIPRVGGELVSSRLVAPWMGIT
                     LLVFAGVSALLFVVRTGTPW"
     gene            complement(1285..2388)
                     /gene="ychF"
                     /locus_tag="PKU75_15535"
     CDS             complement(1285..2388)
                     /gene="ychF"
                     /locus_tag="PKU75_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPF82337.1"
                     /translation="MALTIGIVGLPNVGKSTLFNALTKNNVLAANYPFATIEPNVGVV
                     PLPDARLARLAEIFGSERILPATVSFVDIAGIVKGASEGEGLGNKFLANIREADAICQ
                     VVRAFADDDVVHVDGKVSPKDDIEVIETELILADLQTLEKAVPRLEKEVKAKKAEKAL
                     LDTALAAQGVLEEGKTLYAAGAAAGVDPVLAQSLGLLTTKPFIYVFNLDEDQLADAKL
                     ASSLRELVAPADAVFLNAKLEMDLMEMSDEEALELLQSFGAEESGLDQLARTGFHTLG
                     LQTYLTAGPKESRAWTIHQGWTAPQAAGVIHTDFQKGFIKAEVVSFADLDALGSMAAV
                     KSAGKARLEGKDYVMQDGDVVEFRSGLASGGKK"
     gene            complement(2416..>4171)
                     /locus_tag="PKU75_15540"
     CDS             complement(2416..>4171)
                     /locus_tag="PKU75_15540"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPF82338.1"
                     /translation="MHPDGLLEIGATLHHQGSGSYRVDALETSVPLPMDAVELLDLTG
                     RWCRERSPQRLDLGMGTWLRESRHGRTGHDGPLALCAGDPGFSFRRGRVWAIHLAWSG
                     DSRVYAERSPVGRAQLGAGELLGAGEVILKGGESYRAPTLFAAYSERGLDGVSAAFHG
                     YVRSRPSHPTTPRPVLLNTWEAVYFDHRLDVLTALADKAADAGIERFVLDDGWFGSRR
                     HDGQGLGDWVVSEDLWPDGLTPLIDHVRSRGMEFGIWVEPEMANPDSDLVRAHPDWVL
                     KVPGREPPLWRKQQVIDLANPDCYEHILGRLDALLRDHEIAYVKWDHNRDLIDVAHDG
                     HAAVRGQTLAVYALLDELRRRHPGVEFETCASGGGRVDLGILARTDRLWASDTIDAIE
                     RQQINLWTSLVIPPELIGAHIGGPHSHTTGRVSSFAVRAASALFWHLGVEWNVAELRE
                     SEMAQLQRAVAWHKEHRTLLHTGEVVRPDAPNRNALVHGVVAPDRSAAVFSYAQLATD
                     VTEVAGTVRLDGLDPEAVYAVRPAEIGGLPTCQQMTWPEWVDHPGTRIRGSVLMTVGL
                     SLPNVHPEQAILIELERV"
BASE COUNT          713 a         1425 c         1413 g          620 t
ORIGIN      
        1 tcaacggtct ggaccccgag ggtgtcatct gggtcagggg cctgcttcgg gacctggcct
       61 cggaaggcag gtgcgtcctg ctctcctcgc acctgatgcc cgaactcaag cagtgcgtcg
      121 acgacctggt gatcatccag aacggccgac tggtcgcgaa ggggcatctc gctgagatca
      181 tgtccaccca cgagcacaca gtggtcgtgg acagcgcgga caacggtcga ctcatcgaga
      241 agctcgtggc ggcggcgcgc acgccaccgg cgacgggaat caactcgtcg tccgcaacct
      301 cgaccgctct gccgtgggca ggctggccca ccaagcgggg atcccgctgc gactcttcac
      361 gcaagaacaa ggccgcctcg aggaacgcta cctctcactc gtcggcaccc aggaaggacc
      421 agtcgcacga tgacgcagtc aaatgtctct gccaccggcc tcctgaagtc gcaggtcatc
      481 cgagtcaccc gccagcccgg ccaggtgtgg ggtttcgcag gcgcggctct tcttctcgtc
      541 ttggtcgctg cactcgcctc cgtggcgttc gtccaaggca gtcagagcgg cagcatggag
      601 gtcacaccca gagagagatc cgcgagatcc ccggctcgtc gcgtagcgcc tcgcatgaac
      661 tcgacgcgat aggcgcgcgg ttcacttctt accgcttcca gacgttaaag cgcaaaacgg
      721 aactccaccg cgtcgccctt caccaagggg tacccgtccg aaccacgaac agcagggcag
      781 agacaccggc gaagaccagc aaggtgatgc ccatccaggg tgcgaccaga cggctggaga
      841 cgagctcgcc cccgacccga ggaatcagcg cagcgagccc agccgcctcg atgatcccca
      901 ggccggccaa ggacaggtca tcgaggaaga atgccagggg cacgaagtgc agggccacga
      961 cgagagccac ccaccaggcc atccagcggc ccttacccct gcggtgcagg aacaggcagc
     1021 cgaccaccgc cagcagcacc tccaggccga cgaccagccc gaaccacggg tacctgccct
     1081 ccagcgcact gccctcactc caccgggcga acaccccgta accgaacagc cctgccagca
     1141 ggaggcccat caccgatccg gcgccgagcc aggatcgcca cgccggcggc gggtcctcct
     1201 gaccccagcc gaaccagacg aacgccatca gaccgaacca tgcgatggtg aaggcgtggt
     1261 cgcgaatgaa atcggtgaac acgtctactt cttcccgcca gaggcgagcc cgctgcggaa
     1321 ctcgacgacg tcgccgtcct gcatcacgta gtccttgccc tcgaggcgcg ccttaccggc
     1381 ggacttcacg gcggccatcg acccgagcgc gtcgaggtcg gcgaaggaga cgacctcggc
     1441 cttgatgaag cccttctgga agtcggtgtg gatgaccccg gcggcctgcg gggcggtcca
     1501 gccctggtgg atggtccagg cccgcgactc cttggggccg gccgtcagat aggtctgcag
     1561 gccgagggtg tgaaagccgg tgcgcgccaa ctgatccagg cccgactcct cggcaccgaa
     1621 gctctgcaac agctccaggg cctcctcgtc cgacatctcc atcaggtcca tctcgagttt
     1681 ggcgttgagg aagaccgcgt ccgccggcgc gaccagctca cgcagcgagg aggccagctt
     1741 ggcgtcggcg agctggtcct cgtcgaggtt gaagacgtag atgaaaggct tggtcgtcag
     1801 cagcccaagc gactgcgcga gaacgggatc caccccggcg gcggcgcccg cggcatacag
     1861 ggtcttgccc tcctccagca ccccctgcgc ggcgagcgcg gtgtcgagca gcgccttctc
     1921 cgccttcttg gccttgacct ctttctccag ccgcggcacc gccttctcca gcgtctgcag
     1981 gtcggccagg atcagctcgg tctcgatgac ctcgatgtcg tccttgggcg agaccttgcc
     2041 atcgacgtgc accacgtcgt catcagcgaa ggcgcgcacg acctggcaga tcgcgtccgc
     2101 ctcgcggata ttggcgagga acttgttgcc cagcccctcc ccctcgctcg cgcccttgac
     2161 gatgccggcg atgtcgacga aggacacggt cgcgggcagg atccgctcgg agccgaagat
     2221 ctcggcgagc cgggccaggc gcgcatcggg cagcgggacg acgcccacat tcggctcgat
     2281 ggtcgcgaac gggtagttcg cggcgagcac attgttcttg gtcagggcgt tgaagagggt
     2341 ggacttgccg acattcggca gtccgacaat cccgatggtg agagccacaa ggacaggagt
     2401 ttaccggggc gctgctcaga ctctttccag ctcgatgagg atggcctgct cggggtgcac
     2461 attcggcagc gacaggccga cggtcatcag gaccgacccg cggatgcggg tgcccgggtg
     2521 gtccacccac tccggccagg tcatctgctg gcaggtcggc agcccgccga tctcggcggg
     2581 ccggaccgca tacaccgcct cggggtcgag cccgtcgagc cgcaccgtgc cggcgacctc
     2641 ggtgacatcg gtggcgagct gggcatagga gaagacggcg gcgctgcggt cgggggcgac
     2701 gacaccgtgg acgagcgcat tccgattcgg tgcgtcgggg cggacgacct cgccggtatg
     2761 cagcagggtc cggtgctctt tgtgccacgc caccgcgcgc tgcagctgag ccatctcgct
     2821 ctccctgagc tcggcgacgt tccactccac gcccaggtgc cagaagagcg cgctggccgc
     2881 gcgcaccgcg aaggaggaga cccggccggt ggtatggctg tgtggtcccc cgatgtgtgc
     2941 gccgatcagt tcgggcggga tcaccaggga ggtccacagg ttgatctgtt ggcgctcgat
     3001 cgcgtcgatg gtgtcggagg cccagaggcg gtcggtgcgg gcgaggatgc caaggtcgac
     3061 gcggccgccg ccggaggcgc aggtctcgaa ctccacgccc gggtggcgcc ggcgcagttc
     3121 gtcgaggagg gcatagacgg cgagggtctg cccgcgcacc gcagcgtgac cgtcgtgggc
     3181 gacgtcgatg aggtcgcggt tgtggtccca cttgacgtag gcgatctcgt ggtcgcgaag
     3241 gagggcgtcg agccggccga ggatgtgctc gtagcagtcg gggttggcga ggtcgatgac
     3301 ctgttgcttg cgccacagcg gcggctcgcg gccgggcacc ttgaggaccc aatcggggtg
     3361 tgcgcgaacg agatccgagt cagggttggc catctccggc tcgacccaga tgccgaattc
     3421 catcccgcgg gagcggacgt ggtcgatcag gggcgtgagc ccgtcgggcc aaaggtcttc
     3481 ggagacaacc caatccccga gtccctgacc gtcgtgccgg cgggagccga accagccgtc
     3541 gtcgaggacg aagcgctcga tcccggcgtc ggccgccttg tccgccaggg cggtgaggac
     3601 gtcgagccgg tggtcgaaat agacagcctc ccaggtgttg agcagaaccg gccggggtgt
     3661 cgtcgggtga ctcggccggg atcggacata accgtggaag gccgcgctga caccgtccag
     3721 tccgcgctcg ctgtaggcgg cgaaaagcgt tggcgcgcgg tagctttcgc cgcccttgag
     3781 gatcacctcg ccggccccga gcagctcccc cgcgccgagc tgagcgcgcc cgacggggct
     3841 gcgctcggca tacacgcggg agtctcccga ccaggcaaga tggatcgccc acacccggcc
     3901 gcgccggaag gagaagccgg gatcgccggc gcacagagcc agcggcccgt cgtgcccggt
     3961 gcgcccgtgt cgcgactcgc gcagccaggt gcccatgccc aggtcgaggc gctgcggcga
     4021 gcgttcccgg caccagcgtc cggtcaggtc gagcagctcg accgcatcca tgggcagggg
     4081 cacgctggtc tccagcgcgt ccacgcgata ggagccggat ccctggtggt gcagcgtcgc
     4141 gccgatctcc aacaacccgt ccgggtgcat c
//
LOCUS       DAOPJG010000242         4153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_23599_length_4153_cov_4.682284, whole genome shotgun sequence.
ACCESSION   DAOPJG010000242 DAOPJG010000000
VERSION     DAOPJG010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23599_length_4153_cov_4.682284"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..414)
                     /locus_tag="PKU75_15545"
     CDS             complement(7..414)
                     /locus_tag="PKU75_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82339.1"
                     /translation="MSRQLRVLFLSWRDLHHPEAGGAEKYQREAEATRRGDDAMRAAG
                     EKMSANDFGGAVVLLDAAVADAVAAGVRESEASLRGMLSQALLLAEERPRAAEEARKA
                     KAIAKALGAHDAAESFAQIEKLAVGFIAPKADG"
     gene            620..1753
                     /locus_tag="PKU75_15550"
     CDS             620..1753
                     /locus_tag="PKU75_15550"
                     /inference="COORDINATES: protein motif:HMM:NF022715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3068 domain-containing protein"
                     /protein_id="HPF82340.1"
                     /translation="MRKILGPLLLGLGGFLLTTALLVLLWVPGQVKKTPLNIDSLTTL
                     SGDAVYLDSPRGPVKATSHSVVDGKASDGNTAVFQTMSCLMWNKDGKAPTCTSAEGKG
                     SPLLNAGTDAFATDRRTALAVPDQEKYLGNLASAHQGLINKFPFDVEQKTYPFWDGIL
                     DRAVDAVFVAEEKLDGLDTYKFNINVKDEPAEITKGIQGTYSLDKTMWIDKATGSIIK
                     QSEKQLRQLPDGSPALDMNLAFTDEQVAANIKDAKANGGKLSLVGMLPWIAGLLGLIS
                     LLAGLILNRDKDGTAGEVVRTREPAPDATRVPAGATASTAVASDTRPRTDPEASTRPI
                     STTPAAAGSAGTERPAEGWDLGAGRGEGAGQTARRAEPNSATE"
     gene            complement(1757..2512)
                     /locus_tag="PKU75_15555"
     CDS             complement(1757..2512)
                     /locus_tag="PKU75_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPF82341.1"
                     /translation="MARAVALGIDIGGSGIKGAPVDLKTGEFLQPRLKIATPAKSTPQ
                     AVAKIVGQIADEFTDDLPTDAPIGITIPGVVQHGIVRTAANIDKSWIGAEGEKIFSDT
                     IGRTCLLVNDADAAGVAEQRYGAAKGKQGLVILTTLGTGIGIALLHNGILIPNCEMGH
                     IELAGHDAETRAASSAKDREELSYEEWATKRLQPYYSALENLLWPDLFVVGGGVSRKH
                     EKFLPLLKIRTPIVPATLENKAGIVGAAALAAD"
     gene            complement(2696..3529)
                     /locus_tag="PKU75_15560"
     CDS             complement(2696..3529)
                     /locus_tag="PKU75_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF82342.1"
                     /translation="MPESSHVSRAAVSHEASVTAQRAWWDGEAGDYYADHGAFLGDSG
                     FVWGPEGWTEAELSLLSPAGGMPSDPVLEIGAGAAQCSRWLRDHGVSVVASDLSGGML
                     ARARAIETSGGKPAVPLVQCDGARLPFADEAFEVVFTAYGVVPFVADSARVMSEAARV
                     LRPGGRLVFSTTHPIRWAFPDVPGPAGLTAHQSYWDTRPYVEEDGAGKVTYVEHHRTL
                     GERVREIVAAGLTLTDLVEPTWPDRNDQEWGGWSPTRGRVIPGTAIFVCDKPIRPAGE
                     S"
     gene            3685..>4153
                     /locus_tag="PKU75_15565"
     CDS             3685..>4153
                     /locus_tag="PKU75_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPF82343.1"
                     /translation="MTATTVASTAPQVAINDIGTEEELLAAIDQTIKNFNDGDIVVGN
                     VVKVDRDEVLLDIGYKTEGVIPSRELSIKHDIDPNEVVTVGDEIEALVLQKEDKEGRL
                     ILSKKRAQYERAWGTIEKVKEEDGVVKGTVIEVVKGGLILDIGLRGFLPASLVE"
BASE COUNT          685 a         1453 c         1352 g          663 t
ORIGIN      
        1 cctgggctaa ccgtccgcct tcggcgcgat gaagcccacc gcgagcttct cgatctgcgc
       61 gaagctctcc gccgcgtcgt gcgcgcccag cgccttcgcg atagccttcg ccttccgcgc
      121 ctcctcggcc gcccgcgggc gctcctccgc gaggagcagc gcctgcgaga gcatcccgcg
      181 gagcgacgcc tcgctctccc tcacgcccgc cgcgaccgcg tccgcgaccg ccgcgtcgag
      241 gaggaccacg gcgccgccga agtcgttcgc cgacatcttc tcgcccgccg cgcgcatcgc
      301 gtcgtcgccg cgccgcgtcg cctccgcctc gcgctggtac ttctccgccc cgcccgcctc
      361 gggatggtgc aggtcacgcc aactcaggaa gagcactcgc aattgacggg acacttcaca
      421 ccttgcgtga tctggacggg ctgtcgcggg acctccggca gccgatgctg cgggccgttc
      481 ctacctcagg gtaaccgctg ctggcgactg gttggggcag aaaccgggcc ggttccctat
      541 ctgtctactc gctggtagca tctgccgaca ctgccttacg tcatcggatg ccgcccatct
      601 ggaggagata ctcgcaatca tgcggaaaat tctcggccca ctgctgctcg gcttgggcgg
      661 attcctcctc acgacggcgc tcctggtgct gctgtgggtg cccggtcagg tcaagaagac
      721 cccgctcaac atcgacagcc tgaccacctt gagcggcgac gcggtctacc tcgattcccc
      781 ccgggggccg gtcaaggcaa ccagccactc ggtcgtcgac ggcaaagcat ccgatggcaa
      841 caccgccgtt ttccagacca tgtcctgcct gatgtggaac aaggacggca aggcaccgac
      901 ctgtacctcg gccgagggca agggcagccc gctgctcaat gcgggcaccg acgccttcgc
      961 gaccgaccga cggaccgcat tggcggtgcc cgaccaggag aagtacctcg gcaacctcgc
     1021 gtcggcgcat cagggcttga tcaacaagtt cccgttcgac gtcgagcaga agacctatcc
     1081 cttctgggac ggcatcctcg accgggcggt cgatgccgtc ttcgtcgccg aggagaagct
     1141 cgatgggctc gacacgtaca agttcaatat caacgtcaag gacgagccgg ccgagatcac
     1201 caaggggatc cagggcacgt actcgctcga taagacgatg tggatcgaca aggccacggg
     1261 gtcgatcatc aaacagtccg agaaacagct ccgtcagctt cccgacggca gcccagcact
     1321 ggacatgaac ctcgccttca ccgacgagca ggtggccgcg aacatcaagg acgccaaggc
     1381 caacggcggc aaactcagcc tggtcggcat gctgccgtgg atcgccggtc tgttgggcct
     1441 gatcagcctg ctcgcgggcc tgatcctcaa ccgcgacaag gacggcaccg ccggagaggt
     1501 agtgcgcact cgcgagccgg cgcccgacgc cacccgggtg cccgccggcg cgacggccag
     1561 cactgctgtt gcctccgaca cccggccgcg gaccgacccg gaagcgtcca cccgtcccat
     1621 ctccaccacc cctgctgccg ctggcagtgc cggcacggag cgaccggccg agggctggga
     1681 cctgggcgcc gggcgtgggg aaggcgccgg gcagaccgcc cgtcgggcgg aacccaactc
     1741 ggcgaccgag tagcactcag tccgcggcca gggccgcggc acccacgatg ccggccttgt
     1801 tctcgagcgt ggccgggacg atgggggtgc ggatcttcag caacggcagg aacttctcgt
     1861 gcttgcggga gacgccgccg ccgacgacga acaggtcggg ccacagcagg ttctcgaggg
     1921 ccgagtagta gggctgcagc cgcttagtgg cccactcctc gtaggagagt tcttcgcggt
     1981 ccttggcgct cgacgcggcc ctggtctcgg cgtcgtgccc ggcgagctcg atgtgcccca
     2041 tctcgcagtt ggggatgagt atgccgttgt gcagcaacgc gattccgatg ccggtgccga
     2101 gcgtggtgag gatgacgagg ccctgcttgc ccttggccgc gccataacgt tgctcggcga
     2161 cgccggcggc gtcggcgtcg ttgacgagga ggcaggtgcg gccgatggtg tcggagaaga
     2221 tcttctcgcc ctcggcgccg atccagctct tgtcgatatt cgcggcggtg cgcacgatgc
     2281 cgtgctggac gacgccgggg atggtgatcc cgatgggggc gtccgtgggc aggtcgtcgg
     2341 tgaactcgtc ggcgatctga ccgacgatct tggccacggc ttgcggggtg gacttggcgg
     2401 gggtggcgat cttcaggcgc ggctgcagga actcgccggt cttgaggtcg accggtgcgc
     2461 ccttgatgcc cgaaccgccg atgtcgatac ccagtgctac cgcgcgcgcc atcgctcgct
     2521 ccatctcttc tcgtgtgcag tcggcgtcgc tgccggccga cctgtcgcgg gagatcttag
     2581 ccgcgccggc tgggctgacc ctgctgagcg cgcgggccaa ctcagcagag gttggtcaaa
     2641 tcagcacact ttgctaggtg tgctgatttg accaacctct gctagcagca ggttcctagg
     2701 actcaccggc ggggcgaatc ggcttgtcgc acacgaagat tgcggtgcct ggtatgacgc
     2761 ggccccgggt gggggaccag ccgccccact cctggtcgtt gcggtcgggc cacgtgggct
     2821 cgaccaggtc agtgagcgtg aggccggcgg cgacgatctc gcgcacgcgc tcgccgaggg
     2881 tgcggtgatg ctcgacatag gtgaccttgc cggcgccgtc ctcctcgaca tacggccggg
     2941 tgtcccagta gctctggtgt gccgtcaacc cggcgggccc ggggacgtcg gggaaggccc
     3001 accggatcgg gtgggtcgtc gagaacacca accgccctcc cggccgcagc acccgggccg
     3061 cctcgctcat cacgcgggcc gagtccgcca cgaacggcac caccccgtag gccgtgaaca
     3121 ccacctcgaa cgcctcgtcc gcgaacggca gccgcgcccc gtcacactgc accagcggta
     3181 ctgcaggttt tccgcccgag gtctcgatgg cccgcgcccg ggcgagcatc ccgcccgaca
     3241 ggtcactggc caccacggac accccgtggt cgcgcagcca ccggctgcac tgcgccgccc
     3301 cggcaccgat ctcgaggacc ggatcggacg gcataccccc ggccggggag agcagcgaca
     3361 actccgcctc ggtccacccc tccgggcccc agacgaaccc gctgtcaccg aggaaggcgc
     3421 cgtggtcggc gtaatagtcg ccggcttcgc cgtcccacca cgcgcgctgt gcggtcacgg
     3481 aggcctcatg gctcaccgcg gcgcggctga catggctgga ctcgggcatc ggccaccttc
     3541 gggtttgaca ggattggacg gagtttggca ggtctggacc cggccactag actggcatcg
     3601 cacacgtgtg cgcgttttcg cgtgcgcgcg ggcatgtcca ttatccgcac gattcacctg
     3661 tccacatcga aggcccttct acacatgact gcaactacgg tcgcttcgac cgcccctcag
     3721 gtcgccatca acgacatcgg cactgaggaa gagctgctgg ccgccattga ccagaccatc
     3781 aagaacttca acgacggcga catcgtcgtc ggcaatgtcg tcaaggtcga ccgcgacgag
     3841 gtcctcctcg acatcggtta caagaccgag ggcgtcatcc ccagccgcga gctgagcatc
     3901 aagcacgaca tcgaccccaa cgaggtcgtc accgtcggtg acgagatcga ggccctggtt
     3961 ctccagaagg aggacaagga aggccgcctg atcctgtcca agaagcgtgc gcagtacgaa
     4021 cgcgcctggg gcacgatcga gaaggtcaag gaagaggacg gtgtcgtcaa gggcacggtc
     4081 atcgaggtcg tcaagggtgg cctcatcctc gacatcggcc tgcgcggctt cctgcccgcc
     4141 tccctcgtcg aga
//
LOCUS       DAOPJG010000243         4136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_23721_length_4136_cov_8.278853, whole genome shotgun sequence.
ACCESSION   DAOPJG010000243 DAOPJG010000000
VERSION     DAOPJG010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23721_length_4136_cov_8.278853"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1476)
                     /locus_tag="PKU75_15570"
     CDS             complement(124..1476)
                     /locus_tag="PKU75_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPF82344.1"
                     /translation="MVDVATAPSLLPTLDNARTRELDKKHVLHSWSAQNAINPPPVTE
                     ASGVRMTVDGTSYIDFTSQLVNVNIGYQHPKLVAAIQEQAGRLMTVSPAMANDMRSEA
                     ARVIAEVAPDGMNMVFFTNGGAEANENAIRMARLHTGRHKVLAAYRSYHGATHGAITL
                     TGEPRRFGSEPGIPGVVHFFGPYLYRSPFHATTEAEEGDRALQHLREVMVAEGTDKIA
                     AIILETIVGTNGILMPPDGYLRGVRELCDEFGILLIADEVMAGFARTGEWFAINHWDV
                     VPDLISFAKGVNSGYVPLGGVILSDAVAGSFMDTAYPGGLTYSGHPLACASAVASVSI
                     MKEEGIVEHARDLGERVIGPKLRELAQAHRSVGEVRGIGVFWALELVTDPDARTPLTA
                     DQMNQVAAACKGAGMWPFIAGNRVHIVPPCVITEEEFAEGVRALEAGLAVADGFVAGS
                     "
     gene            complement(1509..2117)
                     /locus_tag="PKU75_15575"
     CDS             complement(1509..2117)
                     /locus_tag="PKU75_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82345.1"
                     /translation="MTLRSRWWLPAAVLVLTMIQLAVGAFADLPQFEGKGFGARLWAY
                     PLMMMLVPAIWFAWRSRGTSEASRGARAAGREGIPWLAFALVMLGFLVDVTGNTLDLY
                     DSVVWWDDANHFVNWIFLNAGLGLLLVPSVEKRWARVLLVTGLGAILAIGWELGEWYT
                     FIRHGTELDTAYQDTLGDEALGTLGAFVAGLLVINHRRRPTS"
     gene            complement(2228..2980)
                     /locus_tag="PKU75_15580"
     CDS             complement(2228..2980)
                     /locus_tag="PKU75_15580"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HPF82346.1"
                     /translation="MSTSAPTRPRAPKRRSPGWVPNQHGAWAMLAAPLLVGILASRPA
                     AVLVPLTAFWFAGYFAFFAVGLWLKSRRKARYFPPVRAYAVISAVLGVVTLVLDPGLA
                     RWAPLFLLPLGVGLVASATRTERSLVSGLATATGSCLMAVVAYDAGGGTDWSRIWIIA
                     AVLAAYFGGTVVYVKTMIRERDSRAHYLLSVTWHALSTLAMLVVSWPLVAVFALLTAR
                     AALFPRRAMTPKTVGLIEIAATIAVALGALAA"
     gene            complement(3007..3555)
                     /locus_tag="PKU75_15585"
     CDS             complement(3007..3555)
                     /locus_tag="PKU75_15585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HPF82347.1"
                     /translation="MHIGIILGSIREGRAGEAVANWVLEQAQSRGDAEYELIDLKSFD
                     IPLLTSATVPGAANKQYDDERVTAWSKAIDACDAFVFVTPEYNHSVPGALKNAYDVLG
                     NEWADKAVGFVSYGAESGVRAVEHWRQIVANFRMVGVRQQVSLSSFLEFGDDGFQPNE
                     RRPQELKTLLDQLTTRAGQLAG"
     gene            3838..>4136
                     /locus_tag="PKU75_15590"
     CDS             3838..>4136
                     /locus_tag="PKU75_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82348.1"
                     /translation="MSKTRSASIVLATALAGTVAFAPSANAASSASAVVVTVCSQGSQ
                     AQLSASSSGGSIAVDFSVSGAPAGHTWLAAIANRGTVVYHGAKVSDANGSWSQSG"
BASE COUNT          683 a         1357 c         1478 g          618 t
ORIGIN      
        1 atcgaaccga cgaccttttc cttaccatgg aaacgctctg ccgactgagc taaggaggcc
       61 tggcgcctcg caaggcgcct gggagagatt acacggtgca gcggcctgcg accaaaacgc
      121 aggtcaggac ccggcgacga acccgtcggc cacggccagc ccggcctcga gcgcacgcac
      181 gccctcggcg aactcctcct cggtgatcac gcacggcggc acgatgtgca cccggttgcc
      241 ggcgatgaac ggccacatgc cggcgccctt gcacgctgcg gcgacctggt tcatctggtc
      301 ggcggtcagg ggcgtgcggg cgtcggggtc ggtgaccagt tcgagcgccc agaagacgcc
      361 gatgccgcgc acctcgccga cgctgcggtg ggcctgcgcc agctcgcgca gcttcggccc
      421 gatgacccgc tcccccaggt cccgggcgtg ctcgacgatc ccctcctcct tcatgatcga
      481 gacggacgcg accgcggagg cgcaggccag cggatgcccg gagtaggtca gaccgcccgg
      541 gtatgccgtg tccatgaacg acccagcgac cgcatcggac aggatgaccc cgccgagcgg
      601 gacatacccg gaattgaccc ccttggcgaa ggagatcagg tcgggaacga cgtcccagtg
      661 gttgatggcg aaccactcgc cggtccgcgc gaacccggcc atcacctcat cggcgatgag
      721 caggatgccg aactcgtcgc acagctcgcg cacgccgcgc aggtagccgt cggggggcat
      781 gaggatgccg ttggtgccga cgatggtctc gaggatgatc gcggcgatct tgtccgtccc
      841 ctcggcgacc atgacctcgc gcagatgctg cagcgcccga tcgccttcct ccgcctcggt
      901 ggtggcgtgg aaggggctgc gatagaggta ggggccgaag aagtgcacga ccccgggtat
      961 gccgggttcg gagccgaagc ggcgcggctc accggtcagg gtgatggccc cgtgggtggc
     1021 gccgtggtag ctgcggtagg ccgcaagcac cttgtgccgg ccggtgtgca ggcgcgccat
     1081 gcggatcgcg ttctcgttgg cctccgcccc gccgttggtg aagaagacca tgttcatgcc
     1141 gtcgggagcg acctcggcga tgacgcgggc ggcctcggag cgcatgtcgt tggccatcgc
     1201 cggggagacg gtcatcagcc ggcccgcctg ctcctggatg gcggcgacca gcttcgggtg
     1261 ctggtagccg atgttgacat tgaccagctg gctggtgaag tcgatatagc tcgtgccgtc
     1321 gacggtcatc cgcaccccgc tggcctcggt gaccggcggc gggttgatgg cgttctgcgc
     1381 ggaccaggag tgcaggacgt gcttcttgtc cagttcgcgg gtgcgcgcat tgtcgagggt
     1441 gggcagcagg ctgggtgctg tggcgacgtc gaccatagtg ttgatcgtat gcggaccgcc
     1501 gccgcgcgtc acgaggtagg gcggcggcgg tggttgatga cgagcagtcc cgcgacgaag
     1561 gcgccgagag tcccgagggc ttcgtcgccg agggtgtcct ggtatgccgt gtcgagttcg
     1621 gtgccgtggc ggatgaaggt gtaccactcc cccagttccc acccgatcgc gaggatcgcg
     1681 ccgaggccgg tcacgagcag cactcgggcc caacgcttct cgacgctcgg cacgaggagc
     1741 agcccaagac cggcgttgag gaagatccag ttgacgaagt ggttggcgtc gtcccaccag
     1801 acgacgctgt catagaggtc gagggtgttg cccgtgacgt cgacgaggaa gccgagcatg
     1861 acgagggcga aggcaagcca cggaataccc tctctcccag cggctctcgc gccccgggat
     1921 gcctcggatg tcccgcggct gcgccaggcg aaccagatcg ccgggacgag catcatcatc
     1981 agcggatagg cccacagccg ggccccgaag cctttgccct cgaactgcgg caggtcggcg
     2041 aaggcgccga ccgcgagctg gatcatggtc agcaccagca ccgccgcggg cagccaccag
     2101 cgcgagcgca acgtcatacg gcattgtggc gcgacacggc cccattgggc agcagatccg
     2161 cctgaccggg ggccgaaccg aattcaccgt ccggctgccg ggatccccgc ggcagcccat
     2221 ggctgcttca ggcggcgagc gcgcccagcg cgacggcgat ggtcgcggcg atctcgatca
     2281 ggccgaccgt cttcggggtc atggcccgac gcgggaagag cgcggcgcgc gccgtgagta
     2341 gcgcaaagac ggcgacgagg ggccagctga cgaccagcat ggcgagcgtg ctcagcgcgt
     2401 gccacgtcac ggacagcaga tagtgggcgc gcgaatcacg ttcgcggatc atcgttttca
     2461 catagacgac ggtgccgccg aagtaggcgg cgagcacagc ggcaatgatc cagatccgtg
     2521 accagtcggt gccgccgccg gcgtcgtagg cgacaaccgc catgaggcag gacccggtgg
     2581 cggtcgccag cccactgacg agcgagcgct cggtgcgcgt ggcggacgcg accagcccga
     2641 ccccgagcgg aagcaggaag agcggcgccc accgggccag acccgggtcc agcaccaggg
     2701 tgaccacacc cagcacggcc gagatgacgg catacgcacg cacgggcggg aagtagcgcg
     2761 ccttgcggcg cgacttcagc cacagcccga cggcgaagaa ggcgaagtag ccggcgaacc
     2821 aaaacgccgt cagcggcacg aggacggccg cggggcggct ggccaggatg ccgacgagca
     2881 agggggcggc gagcatcgcc cacgcgccgt gctggttggg cacccacccg ggcgagcgcc
     2941 gcttgggcgc gcgcgggcgg gtaggtgccg aggtgctcac gggtttaggg tatgccgcgt
     3001 cctgcgtcag ccggcgagct gacccgcgcg ggtggtgagc tggtccagga gggtcttcag
     3061 ttcctgcgga cggcgctcgt tgggctggaa gccatcgtcg ccgaactcca ggaagctgga
     3121 gagggagacc tgctggcgca cgccgaccat ccggaagttg gcgacgatct ggcgccagtg
     3181 ctcgaccgcc cggacccccg actcggcgcc gtaggagacg aacccgaccg ccttgtcggc
     3241 ccactcgttg ccgagcacgt cgtaggcgtt cttcaaggca cctgggacgg agtggttgta
     3301 ctcgggtgtg acgaagacga aggcgtcgca cgcgtcgatg gccttgctcc acgcggtgac
     3361 ccgctcgtcg tcatactgct tgtttgcggc gccggggacc gtcgcgctcg tcaggagagg
     3421 gatgtcgaag gacttgagat cgatgagctc gtactcggcg tcaccacgcg actgggcctg
     3481 ctcgagaacc cagttcgcga cggcttcgcc agcgcggcct tcgcggatgg agccgagaat
     3541 gatgccgatg tgcatggggg tgctcctcag agagatgggt tgactgtcct cagcgggaca
     3601 cttgaacaat caaccatagc gtggctatcc cagatcgggg cgcgacgacc agcgcatcgc
     3661 gggcggtatg tacgtgcaga tgggtcgcga tacgtcccga gtgcgtgcgg agcagagcgc
     3721 gacccgcagg tcttggggtg cgcttgggta tgacgtgggg tgttgcccgg ttggcagcct
     3781 cggattctct ggttaccggt tcgtagctag cgggcccacg tgatgaaagg acaccccatg
     3841 tcgaaaaccc gttccgcctc catcgttctt gcgactgccc tggccggcac cgtggccttc
     3901 gcaccgagcg ccaatgccgc ctcgtccgcg tcggcggtcg tcgtcaccgt ctgcagccag
     3961 ggttcgcagg cgcagctgag cgcctcgtcg agcggcggct cgatcgcggt ggacttctcc
     4021 gtctccggcg cccccgccgg ccacacctgg ctcgccgcca tcgccaaccg cggcacggtc
     4081 gtctaccacg gggcgaaggt ctccgacgcc aacggctcat ggagccaatc cggcag
//
LOCUS       DAOPJG010000244         4091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_24076_length_4091_cov_9.018335, whole genome shotgun sequence.
ACCESSION   DAOPJG010000244 DAOPJG010000000
VERSION     DAOPJG010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24076_length_4091_cov_9.018335"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PKU75_15595"
     CDS             <1..936
                     /locus_tag="PKU75_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82349.1"
                     /translation="GAVLGGGILGLVVALTWLAGQCAQSGARGVCGLREALRSIRIED
                     LRTHAVRTTHVGGAVLAGDLRAVRLDVAAPVTRARSVRLRPGGRYTTVVRRDLLGYRR
                     SPYAVALGVLGLAGAFALTAVASTAPAAPVVLGIVAMLVAYFAFGALTEGVRLVADTA
                     GTPPLFGLPFRSEALAHLVAPVVTALTVGLPVTAVVARVLGGRVGVALAACGIVVGIL
                     AGTSLMAAFRGSPPEPAFVPEVGPMMMVYWYARPLVLAAVSGGLFLAGLADLSDRTDP
                     AGLWRLVLLSGAVLAAFSLAYGIYLARKLELGHRV"
     gene            966..1787
                     /gene="rsmI"
                     /locus_tag="PKU75_15600"
     CDS             966..1787
                     /gene="rsmI"
                     /locus_tag="PKU75_15600"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPF82350.1"
                     /translation="MPLILAATPIGDPRDASARLIEELGSADVVAAEDTRRTKRLCAA
                     LGVTPTGRMLSYHEHNEAAVTADLIARLQAGERVLLVTDAGMPSVSDPGYRLVAAAVA
                     AGLPVTCAPGPSAVLMALALSGLPVDRFAFDGFLPRKPGERTRALAELAAERRTMVFF
                     EAPHRTPATLAAMAEAFGADRPAAVCRELTKTYEEVRRDNLGRLAEWAADGVLGEITI
                     VVAGAPPAAADLPSAVAQVQDLVAAGTRLKDASAEVAALTGLSKKALYDAALAAR"
     gene            complement(1815..2513)
                     /locus_tag="PKU75_15605"
     CDS             complement(1815..2513)
                     /locus_tag="PKU75_15605"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HPF82351.1"
                     /translation="MTDDRPRTLARAQTSRGELALRVRDGLQGPVHELIVAGVFAMDD
                     AEMSTEIELARAGLTGCANPRRVLVGGLGLGHTTLAVLADERVVSVTVVEIEDTLVAW
                     AREGLVPTLTRVAADPRVHLVAADVGDHLGAEGAAYDAILLDVDNGPSFLVHQHNAGL
                     YAETSLRAMLRRVAPGGILAIWAAQLEPGLLARLTLLAAGDPGVTVEERVLPIEREGR
                     SFDYAIYVVSRIDQ"
     gene            complement(2630..3592)
                     /locus_tag="PKU75_15610"
     CDS             complement(2630..3592)
                     /locus_tag="PKU75_15610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPF82352.1"
                     /translation="MTRPAIVVAGDSLIDFEPIRLPAGDVAYRPHCGGSCLNVAIGLG
                     RLDVPTAFLGRLSTDHFGRMLRAHLAEAGVGTDLILETDDLTTLAMVHLSEGGQPSYS
                     FHASGAADRGLTRQHLTESSWGGTLPEGAALHIGSIALAMEPQASTLTELFLGEAGRR
                     VLTLDPNVRPSLVTDHETFVSRIEALARVSTIVKCSDEDCAAIAPGAPPGQVMRRWLD
                     EGVSLVVLTEGSKPVRAWTRHASAAYQPPPVPVIDTIGAGDSFMAALLADLHHTGRLS
                     VAAIEALDDAALETILTWAGTAATHTVQQAGANPPWTTDLPAAT"
     gene            complement(3597..>4091)
                     /locus_tag="PKU75_15615"
     CDS             complement(3597..>4091)
                     /locus_tag="PKU75_15615"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPF82353.1"
                     /translation="EGYYETPEGLPVVRRAGSDLTIATYGATLYRAMEAADLLQERHG
                     LSAEVVDLRFLCPLDYEPLLESVRKTHRLLLASDAVQRGSFLQQVAADVQTLAFDVLD
                     APVTVVGSRNHVTPAPELEDIFFPQAEWILDAVHERIVPLPGHTPTSNQTEGELMRRS
                     RLGV"
BASE COUNT          534 a         1427 c         1514 g          616 t
ORIGIN      
        1 ggcgcggtcc tcgggggcgg gatcctcggg ctggtggtgg ccctgacctg gctggcggga
       61 cagtgtgcgc agtccggcgc tcgcggggtg tgtgggttgc gagaggcgtt gcgcagcata
      121 cggatcgagg acctgcgcac ccacgctgtg cgcacgacgc acgtcggcgg ggcggtgctc
      181 gcgggggacc tgcgcgcggt gcgcctcgac gtggcggcgc cggtcacccg ggcccggtcg
      241 gtgcgcctgc gtccgggcgg gcgctacacc acggtggtgc gccgggacct gctcggctat
      301 cgccggtcac cgtatgccgt cgccctgggc gtcctcggtc tcgccggggc gttcgcgctc
      361 accgcggtgg catccacggc gcccgccgcg ccggtcgtct tgggcatcgt ggccatgctg
      421 gtcgcgtatt tcgccttcgg cgccctgacc gagggagtcc ggctggtcgc cgacaccgcg
      481 gggacgccgc cgctgttcgg gttgccgttc cgatccgagg cgctcgcgca cctggtggcg
      541 ccggtggtga cggcgctgac ggtgggtctg ccggtgaccg ccgtggtggc gcgcgtgctg
      601 ggcggccggg tcggggtggc actcgcggcg tgcggcatcg tcgtgggcat ccttgccggg
      661 acttcgctca tggcggcctt tcgcggctcg ccgcccgagc cggccttcgt gccggaggtc
      721 ggcccgatga tgatggtcta ttggtatgcc cgccccctcg tcctggcggc ggtgagcggt
      781 ggcctgttcc tcgccggtct tgccgacctg tccgaccgga ccgacccggc cgggctgtgg
      841 cggctggtgc tcctctctgg cgccgtcctg gccgccttct ccttggccta cgggatctac
      901 ctggcccgca agctcgaact cggccatcgg gtctagggcg ccccgcggcc atgcatgatg
      961 gcgctatgcc tctcatcctg gccgcgaccc ccatcggcga tcctcgcgac gcgagcgccc
     1021 ggctgattga ggaactcggg agcgccgatg tcgttgcggc agaggacacc cggcgcacga
     1081 agcgactgtg cgcggccctc ggagtcacgc ccacagggcg gatgctcagc tatcacgagc
     1141 acaacgaggc cgccgtcacc gccgacctga tcgcccgact ccaggcgggg gagcgggtgc
     1201 tgctcgtgac ggacgccggt atgccgtcgg tgtccgaccc cggctaccgc ctcgtcgccg
     1261 cggcagtcgc cgccggcctg ccggtcacct gcgcgcccgg gccgagcgcg gtcctgatgg
     1321 cgctggcgct ctccgggctg ccggtggacc gcttcgcgtt cgacggcttt ctcccgcgca
     1381 agccggggga gcggacccgg gctctcgccg agctcgccgc cgagcggcgc acgatggtct
     1441 tcttcgaggc gccgcaccgc accccggcga ccctggccgc gatggccgag gccttcggtg
     1501 ccgaccgccc ggcagccgtc tgccgcgagc tgaccaagac gtatgaggag gtgcgccgcg
     1561 acaatctggg ccggctcgcg gagtgggcag ccgacggggt cctcggggag atcaccatcg
     1621 tcgtggccgg cgcgccaccg gcggccgcgg acctgccctc cgccgtcgct caggtccagg
     1681 acctcgttgc ggccggcacc cggctcaagg acgccagcgc cgaggtcgcg gcgctgacgg
     1741 ggttgtccaa gaaggcgttg tacgacgctg ccctggccgc ccggtgaccg gcgcgcagac
     1801 gcacgtccgg gctatcactg gtcgatccgg gacaccacat agatggcgta gtcgaacgag
     1861 cggccctccc gttcgatcgg caagacccgc tcctcgacgg tgacgccggg gtcacccgcg
     1921 gcgaggagtg tgagccgggc cagcaggccg ggttcgagct gagctgccca gatcgcgagg
     1981 atcccgccgg gggcgactcg ccgcagcatc gcgcggaggc ttgtctcggc atacaacccc
     2041 gcattgtgtt ggtgcacaag gaaactgggc ccgttgtcca catcgagcag gatcgcgtcg
     2101 tatgccgcgc cctccgcgcc gagatggtcc ccgacgtcgg cggcgacgag gtggacgcgc
     2161 gggtctgcag cgacccgggt gagcgtcggg accaggccct cgcgggccca cgcgacgagc
     2221 gtgtcctcga tctcgaccac cgtcacggag acgacccgct catccgccag caccgccagc
     2281 gtcgtgtgcc ccagtcccaa gccgccgacg aggacccggc ggggattcgc gcaccccgtc
     2341 agccctgcgc gggccagttc gatctcggtc gacatttcag cgtcgtccat cgcgaacacc
     2401 ccggccacga tgagttcgtg caccggcccc tgcaggccgt cgcgaactcg caacgccagc
     2461 tcgccgcgcg acgtctgcgc gcgcgccagc gtgcggggcc ggtcgtcggt caccgggcga
     2521 tgctcagacg acactggtcg ctcggacact ggtcgctcgg acactggttg ctcggacact
     2581 ggttgctcag acacaggttg ctcagacaca ggatgctgga caccagcggt caggtggcgg
     2641 cgggcaggtc cgtggtccag ggcgggttgg cgcccgcctg ctggaccgta tgcgtcgcag
     2701 ccgtgccggc ccatgtcaag atcgtttcca gcgcagcatc atccagcgcc tcgatcgccg
     2761 cgacgctgag ccggccggtg tggtgcaggt cggccaggag cgcggccatg aacgagtccc
     2821 cggcgccgat ggtgtcaatg acgggcacgg gcggcggctg gtaggccgcc gacgcgtgcc
     2881 gggtccaggc acgaacgggt ttgctccctt cggtcagcac gacgagactg accccctcgt
     2941 cgagccagcg gcgcatcacc tgccccggcg gcgcgccggg ggcgatggcg gcgcagtcct
     3001 catcgctgca cttgacgatc gtgctcacgc gagcgagcgc ctcgatgcgg gagacgaagg
     3061 tttcgtgatc ggtgacgagg gagggccgca cgttggggtc gagggtgagc acccggcgac
     3121 cggcctcgcc gaggaagagc tcggtcaagg tgctcgcctg cggctccatc gccaaggcga
     3181 tggatccgat gtgcagcgct gctccctcgg gaagcgttcc gccccaggag gattcggtca
     3241 ggtgctggcg cgtgaggccg cggtccgcgg cgccggaggc gtggaaggaa taggacggct
     3301 gcccgccctc gctcaggtgc accatggcga gtgtcgtgag gtcgtcggtc tcgaggatca
     3361 ggtcggtgcc gacgccggcc tcggccagat gggcgcgcag catccggccg aagtggtcgg
     3421 tggataaccg cccgaggaat gccgtcggca cgtccaggcg gccgaggccg atggcgacat
     3481 tgagacacga accgccgcag tgcggacgat aggcgacatc gcccgcgggc aaccggatcg
     3541 gttcgaagtc gatcaggctg tcgccggcga cgacgatggc cggacgcgtc atgcggctac
     3601 accccgagcc ggctgcggcg catcagttcg ccctcggtct ggttgctcgt gggcgtatgc
     3661 ccgggcagcg gcacgatccg ctcgtggacg gcgtcgagga tccactcggc ctgcgggaag
     3721 aagatgtcct ccagctctgg ggcgggggtg acgtggttgc gcgagcccac gaccgtgacc
     3781 ggtgcgtcca ggacgtcgaa ggccagggtc tggacatcgg cggcgacctg ctgcaggaac
     3841 gaaccccgtt gcaccgcatc ggaagccagc aacaggcggt gggtcttgcg caccgactcc
     3901 agcagcggct cgtagtcgag cgggcacagg aatcgcaggt cgaccacctc ggccgacagg
     3961 ccgtggcgct cctgcaggag gtcggccgcc tccatcgccc gatagagcgt cgcgccgtat
     4021 gtcgcgatcg tcaggtcgga tccggcccgc cgcacgacgg gcaggccctc gggggtctcg
     4081 tagtagccct c
//
LOCUS       DAOPJG010000245         4071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_24241_length_4071_cov_7.351096, whole genome shotgun sequence.
ACCESSION   DAOPJG010000245 DAOPJG010000000
VERSION     DAOPJG010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24241_length_4071_cov_7.351096"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..2104
                     /gene="typA"
                     /locus_tag="PKU75_15620"
     CDS             209..2104
                     /gene="typA"
                     /locus_tag="PKU75_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPF82354.1"
                     /translation="MPTQSRSDVRNVAIVAHVDHGKTTLVDKMLWQAGAFGAHQHVDE
                     RAMDSGDLEREKGITILAKNTAIRYAGAAAAEAGHPEGVTINIIDTPGHADFGGEVER
                     GLSMVDGVVLLVDASEGPLPQTRFVLRKALAAHMPVVLCINKVDRSDARIAEVEDEVY
                     ELFMDLMEDGGNAEHVLDFPIVYASAKAGRASLTRPADGELPDSEDLEPLFATILSTI
                     PAPQYDDGLPLQAHVTNLDASNFLGRLALLRVHNGRISKGQQVAWCRHDGSVQKVKIT
                     ELLMTEALERKPVDSAGPGDIIAIAGIPEIMIGDTLTDPDDPRPLPLITVDEPAISML
                     IGTNTSPMAGRVRGAKVTARLVKDRLDRELVGNVSLRVLPTERPDAWEVQGRGELALA
                     ILVEQMRREGYELTVGKPQVVTREVDGKVHEPVEQLTIDAPEEFLGTITQVLAQRKGR
                     MTQMTNHGTGWVRMEFLVPSRGLIGFRTEFLTETRGTGIAHHVFHGYEPWAGAIVTRL
                     TGSLVADRSGVATSYAMFNLQERGTLLVEPTTEVYEGMIVGENSRADDMDVNITKEKK
                     LTNVRSSTADVLERLIPPRRLSLEQSLEFCREDECVEVTPEAIRIRKVELDQNARARA
                     AARAKNA"
     gene            2109..2612
                     /locus_tag="PKU75_15625"
     CDS             2109..2612
                     /locus_tag="PKU75_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPF82355.1"
                     /translation="MTAYRTQPAGADDFRAVMSRLVSGVVLLSAHHDGHDHAMTATAF
                     TSVSLDPPLVLACVEQAARFHELVTDSGVWGVSFLGRKQRAAAEWLATPGRPLVGQFD
                     AIPHWRAESGVLLFTGSLATLEARTTAIHQAGDHSIIVGEVIATGLDDDPDDALVHYR
                     SRYGSIA"
     gene            2609..>4071
                     /locus_tag="PKU75_15630"
     CDS             2609..>4071
                     /locus_tag="PKU75_15630"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPF82356.1"
                     /translation="MSTTRPLPTTPAQAPRRRRRWPWVVASLGVLGAAGYAAAALTLG
                     SKTASDTTVGGVQVGGMTHAEALDAVRTGLQDKAKAKVPVAFGEQQANLDPATAGLSV
                     DYAASVDGLTGASFNPVALYDRLTGSVERDPVLAVDDGKLTAALTEIAETIAVEPKNA
                     RLVVKDGKAVVTDAVDGVAADVAKTADAVTTGWLKVGKINGALTAQEADVTTEEAESA
                     KREVADKVLSGPVTISVDGKDFPLAADAIGKTLSFTETNGDLAPSYDEKALVKAVRAA
                     GTKAGVLKEAKDAVVSSANGSFHVEPSVDGLDVDADAVTAAVEKAAVSDDRVATVAAK
                     AAPAKFTTKEAKDSLPTGVISTFTTHFPYAPDRTHNITLAAKRLNGAYVAPGETFSLN
                     AWLGQRTPEKGYRGAPVIYNGRLTKDYGGGISQVSTTLFNAIFFSGAQIDQYTPHSFY
                     ISRYPEGREATISWPNVDNRFTNTTKGGILIQASVGP"
BASE COUNT          695 a         1442 c         1356 g          578 t
ORIGIN      
        1 ccggcgaccg tcgcgcgggc gtggggacgc atcccgccgt tgaccgcgaa gtaggctccg
       61 cgagcgggac gttcgcccaa gaggtcggtc atcggtggaa cgcgacatgg actcgctcat
      121 cgccaccggc gccgcccggc cgacacagtc ctgctgaccc cgtttcgcgg attcgtcggt
      181 atgccgtttg gcctgagaga atcgggtaat gcccacgcag tctcgcagcg acgtccgtaa
      241 tgtcgcgatc gtcgctcacg tcgaccacgg caagaccacc ctcgtcgaca agatgctctg
      301 gcaggcgggc gctttcggcg cccaccagca cgtcgacgag cgagcgatgg actccgggga
      361 cctggagcgc gagaagggca tcacgattct cgccaagaac accgcgatcc ggtatgccgg
      421 tgccgcggcg gcggaggccg gtcaccccga gggcgtgacg atcaacatca tcgacacccc
      481 ggggcacgcc gacttcggcg gcgaggtcga gcgcggcctg tcgatggtcg acggggtggt
      541 gctgctcgtc gatgcgtccg agggcccgct gccacagacc cggttcgtgc tgcgcaaggc
      601 gctggccgcg cacatgccgg tcgtgttgtg catcaataag gtcgaccgct ccgacgcccg
      661 gatcgccgag gtggaggacg aggtctacga gctgttcatg gacctgatgg aggacggcgg
      721 caacgccgag cacgttctcg acttcccgat cgtctacgcc tcggccaagg cgggccgcgc
      781 gtcgctgacc cgcccggcgg acggcgagct gcccgacagc gaggacctcg agccgctgtt
      841 cgcgacgatc ctgtcgacga ttccggcgcc gcagtatgac gacggcttgc cgctgcaggc
      901 ccacgtcacc aacctcgacg cctccaactt cctcggccgg cttgcgctgt tgcgggtcca
      961 caacgggcgg atcagcaagg ggcagcaggt cgcctggtgc cgtcatgacg gctcggtcca
     1021 gaaggtcaag atcaccgaac tgctcatgac cgaggcgctc gaacgcaagc cggtcgactc
     1081 cgccggcccg ggcgacatca tcgccatcgc gggcattccc gagatcatga tcggcgacac
     1141 gctcaccgac cccgacgacc cgcggccgct gccgttgatc accgtcgacg agccggccat
     1201 ctccatgctc atcggcacca acacctcgcc gatggccggc cgggttcgcg gcgccaaggt
     1261 caccgcccgg ctcgtcaagg accggctcga ccgcgaactc gtcggcaatg tgtcacttcg
     1321 ggtgctcccg accgagcggc cggacgcctg ggaggtccag ggccgtggcg aactggcgct
     1381 ggcgatcctc gtcgagcaga tgcgccgcga gggctatgag ttgaccgtcg gcaagccgca
     1441 ggtcgtcacc cgcgaggtcg acggcaaggt gcacgagccc gtcgaacagc tgaccatcga
     1501 cgctcccgag gagttcctcg gcacgatcac ccaggtgctg gctcagcgca agggccggat
     1561 gacgcagatg accaaccacg gcaccggctg ggtgcggatg gaattcctcg tcccctcccg
     1621 cggcctgatc gggttccgca ccgaattcct caccgagaca aggggaaccg gcatcgccca
     1681 ccacgtcttc cacggctacg agccgtgggc cggcgcaatc gtcacccgct tgaccggctc
     1741 cctggtggcc gaccgctcgg gtgtcgccac gtcatacgcc atgttcaacc tgcaggagcg
     1801 cgggacgctc ctggtggagc cgaccaccga ggtctacgag ggcatgatcg tcggcgagaa
     1861 ctcccgcgcc gacgacatgg acgtcaatat caccaaggag aagaagctca ccaacgtgcg
     1921 ctcctccacc gccgacgtgc tcgagcggct gatcccgccg cggcggctca gcctggaaca
     1981 gtcgttggag ttctgccgcg aggacgagtg cgtcgaggtc acccccgagg cgatccgcat
     2041 ccgcaaggtc gaactcgacc agaacgcccg agcgcgcgcc gcggcgaggg ccaagaacgc
     2101 ctgagcggat gacggcatac cgaacgcaac ctgccggcgc ggacgacttc cgtgccgtca
     2161 tgtcgcggct cgtcagcggc gtcgtgctgc tcagcgccca ccatgacggg catgaccacg
     2221 cgatgacggc gacggcgttc acgtccgtct cgctcgaccc gccgctcgtg ctcgcgtgcg
     2281 tcgagcaggc cgcgcgcttc cacgaactgg tcaccgactc cggcgtctgg ggggtctcct
     2341 tcctcggccg caagcagcgg gccgccgcgg agtggctggc cacgcccggc cggccgcttg
     2401 tcggccagtt cgacgcgatt ccgcactggc gcgccgagag tggcgtcctg ctcttcacgg
     2461 gtagcctcgc taccctcgaa gcccggacca cggccataca tcaggccggg gaccactcca
     2521 tcatcgtcgg cgaggtcatc gcgaccggcc tcgacgacga cccggacgac gcgctcgtcc
     2581 actaccgcag ccgctacggg agcattgcat gagcaccaca cgaccccttc cgacgacgcc
     2641 cgcccaggcg ccgcgccgcc ggcgccgctg gccctgggtc gtggcctcgc tcggtgtgct
     2701 cggggcggcc gggtatgccg cggccgcgct cacgctcggg tcgaagacgg ccagcgacac
     2761 cacggtcggt ggggtacagg tcggcggtat gacgcatgcc gaggccctcg acgcagtgcg
     2821 gacagggttg caggacaagg cgaaagccaa agtgcccgtc gcgttcggcg agcagcaggc
     2881 caacctcgat ccggcgacgg cgggcctgtc cgtcgactac gccgcgagcg tcgacggcct
     2941 caccggcgcg agcttcaacc ccgtcgcgct gtatgaccgg ctgaccggct cggtggagcg
     3001 cgacccggtc ctcgccgtgg acgacggcaa gctcacggcc gcgttgaccg agatcgccga
     3061 gacgatcgcg gtggagccca agaacgccag gctcgtcgtc aaggacggca aagcggtcgt
     3121 gacggacgcg gtggacgggg tcgcggctga tgtcgcgaag acggccgacg cggtcacgac
     3181 cggatggctg aaggtcggca agatcaacgg ggcgctgacg gcgcaggagg ccgacgtgac
     3241 gaccgaggag gccgagtcgg ccaagcgcga ggttgccgac aaggtgctct ccggcccggt
     3301 cacgatctcc gtggacggca aggatttccc gctcgctgcc gacgccatcg gcaagacgtt
     3361 gagcttcacc gagaccaacg gtgacctggc gccttcctac gacgagaagg ctctcgtcaa
     3421 ggcagtacgg gcggccggca ccaaggccgg tgtgctcaag gaggccaagg atgccgtcgt
     3481 ctcgtcggcc aacggctcct tccatgtcga accgtccgtc gacgggctgg acgtcgacgc
     3541 cgacgccgtg acggccgccg tggagaaggc cgcggtgagc gatgatcgcg tcgccacggt
     3601 ggcagccaag gccgcgccgg cgaagttcac gacgaaggag gccaaggact cgctgccgac
     3661 cggcgtcatc tccaccttca ccacccactt cccctacgcg ccggaccgga cgcacaacat
     3721 caccctcgcc gcgaagcgac tcaacggcgc ctatgtcgct ccgggagaga ccttttcgct
     3781 gaacgcctgg ctcgggcagc ggaccccgga aaagggctat cgcggcgcgc cggtcatcta
     3841 caacggccgg ttgacgaagg actacggggg tggcatctcg caggtgtcga ccaccttgtt
     3901 caacgcgatc ttcttctccg gcgcgcagat cgaccagtac accccgcaca gcttctatat
     3961 ctcccgctac cccgaaggcc gtgaggcgac catcagctgg ccgaacgtcg acaaccgctt
     4021 caccaacacg accaagggcg gcatcctcat ccaggcctcg gtcggcccgg a
//
LOCUS       DAOPJG010000246         4071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_24242_length_4071_cov_7.139691, whole genome shotgun sequence.
ACCESSION   DAOPJG010000246 DAOPJG010000000
VERSION     DAOPJG010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24242_length_4071_cov_7.139691"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="PKU75_15635"
     CDS             <1..1592
                     /locus_tag="PKU75_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF82357.1"
                     /translation="RFQQVFVGEPSVEDTIAILRGLKERYEAHHKVEIEDSALVAAAD
                     LSDRYISGRQLPDKAIDLVDEAASRLRMEIDSSPVEIDQLRRSVDRMKMEELHLAKES
                     DDASKERLAKLRKDLADATEELNALESRWEAEKSGLNTIGDLKAQLDELRTQADRLQR
                     EGNYEQASKLLYGDIPALEKQLAIANENEIARGATDLMVKERVGPDDIAEVISAWTGI
                     PAGRLLQGETEKLLSMESIIGARLIGQGSAVQAVSDAVRRSRAGLADPDRPTGSFLFL
                     GPTGVGKTELAKALADFLFDDERAMVRIDMSEYSERHAVARLIGSPPGYVGYDEGGQL
                     TEAVRRRPYSVVLFDEIEKAHPETFDILLQVLDDGRLTDGQGRTVDFRNVILVMTSNL
                     GSQFLIDPMLSAEQKKEAVMSTVRQAFKPEFLNRLDDVIIFDPLSQDELARIVELQVR
                     QLASRLHDRRITLEVTDAAREWLAKTGYDPAYGARPLRRLVQREIGDRLARAVLAGQV
                     RDGDTVTVDLAPDGDSLTVAA"
     gene            1711..2397
                     /locus_tag="PKU75_15640"
     CDS             1711..2397
                     /locus_tag="PKU75_15640"
                     /inference="COORDINATES: protein motif:HMM:NF018822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HPF82358.1"
                     /translation="MRRRAVLAAVLLPVAVVACSGEKKAAADPAAALAAAKTTLDGAQ
                     AVTLDLKSDKPIPSGHNGVSAAKGTGLIDPTTPKFQGTVSAVVSGAPVTTDVITIGDT
                     TWMKLFTPTFTKVDLKALNAPNPGSFFKPDTGLSQILAKTSDAAGGTEKRFGKEVLTA
                     YTGKVPGAVVKSLLNLGADTSTYAAEFGIAEGNQLRTATLTGPFYDTGNSSYEIVLTD
                     YGKTVEIAQP"
     gene            2402..>4071
                     /locus_tag="PKU75_15645"
     CDS             2402..>4071
                     /locus_tag="PKU75_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF82359.1"
                     /translation="MTAPAPAARARPRALLGWAAVAVALAAADTYVVVLALTEMMQGV
                     GLGIENLQRAAPIISGFLLGYVAVLPLIGRLADLVDRQRVLMWCLAIFTIGSGLTALA
                     VELPVLVAGRVIQGLGGGGLVPATLAIVAASWPPHERGMPLGLVGAVQELGSVVGPVL
                     GAAVLAVADWRMIFWLNAIAGIVLAAVIRRGGRATAGPGEPPSPRAGGRQGWALPVVW
                     LLALATLWLALAAPDRLATDVTFGVPFVPFGSTISRLTTPVGAAALSLVTLALAWSLR
                     RAWPILHQAGPFGAAMLAGALGSVILTFAAAQPEKEIVGPLGYALLPVAAVCGAAYVL
                     HHRRAENPVIPHGVVRGRTAWALVVSFCVGAALVAVIVNIPLLARVANAADETAAAFV
                     LLRFLLAVPVGALLGGWSLRRLGDGVVSATGLALATLGLGLMTTWGRGVFDTAGAWIV
                     LALSGFGIGLALAPVNNAALAAVREEAHGAASALVVVARMVGMVLGLALLTAIGLHRY
                     FAIVAVLPDPTDKEALKDAAIAQVHWVFAGAAVAAALGALASLRLGLRR"
BASE COUNT          572 a         1540 c         1343 g          616 t
ORIGIN      
        1 gccgcttcca gcaggtcttc gtcggcgagc cgagcgtcga ggacaccatc gccattctcc
       61 gcggcctgaa ggagcgctac gaggcgcacc acaaggtcga gatcgaggac agcgcgctcg
      121 tcgccgcggc cgatctctcc gaccgttaca tcagcggccg ccaactgccc gacaaggcca
      181 tcgacctcgt cgacgaggcg gcctcccgcc tgcgcatgga gatcgactcc agccctgtcg
      241 agatcgacca gctgcgccgc tccgtcgacc ggatgaagat ggaggagctg cacctggcca
      301 aggagagcga cgatgcctcg aaggagcgcc tggccaagct ccgcaaggac ctggccgacg
      361 ccaccgaaga actgaacgcc ctcgaatcac gttgggaggc agagaagtcc ggcctcaaca
      421 cgatcggtga cctgaaggct cagctcgacg agctgcgcac ccaggccgat cggctgcagc
      481 gcgagggcaa ctacgagcag gcctccaagc tgttgtatgg cgacatcccg gcgctcgaga
      541 agcaactcgc gatcgccaac gagaacgaga tcgcccgcgg agccaccgac ctgatggtca
      601 aggagcgcgt cggtcccgac gacatcgccg aggtcatctc ggcgtggacc ggcatccccg
      661 ccggccgact cctgcagggc gagaccgaga agctgctgtc gatggagtcg atcatcgggg
      721 cccgcctgat cggccagggc agtgccgtgc aggccgtctc cgacgccgtg cggcgtagcc
      781 gtgccgggct cgccgacccc gaccggccca ccgggtcctt cctcttcctc ggccccaccg
      841 gtgtcggcaa gaccgaactc gcaaaggcgt tggcggactt cctcttcgac gacgagcggg
      901 ccatggtccg catcgacatg tcggagtact ccgagcgcca cgccgtcgcg cgcctgatcg
      961 gctccccgcc cggctatgtc ggctacgacg agggcggcca gctgaccgag gcggttcgcc
     1021 gccggcccta cagtgtcgtc ctcttcgacg agatcgagaa ggcccacccg gagaccttcg
     1081 acatcctcct gcaggtgctc gacgacggcc gcctgaccga cggccagggc cgcacggtcg
     1141 acttccgaaa tgtcattctc gtgatgacct ccaacctcgg cagccagttc ctcatcgacc
     1201 cgatgctgag cgcggagcag aagaaggagg ccgtcatgtc gacggtgcgc caggccttca
     1261 agcccgagtt cctcaaccgg ctcgacgacg tcatcatctt cgacccgctc agccaggacg
     1321 aactcgcccg catcgtcgag ctccaggtcc gccagctcgc cagtcgcctg cacgaccggc
     1381 ggatcacgct ggaggtcacg gacgccgcgc gggagtggct ggccaagacc ggctacgacc
     1441 cggcatacgg cgcccgccca ctccgccgcc tggtccagcg cgagatcggc gaccggctcg
     1501 ctcgtgccgt cctggccggg caggtgcgcg acggcgacac cgtcacggtg gacctggccc
     1561 ccgacggcga ctccctgacc gtcgccgcct gaccgcccgg ccttccttcc agggggtccg
     1621 tatacgtggg ctcgagcctg cctaccatcg ccgcctggtc gccaccgaag tgaccgggat
     1681 cgatcacccg cagcgggtat cgttcgcctc atgcgtcgtc gtgccgtcct cgcagccgtg
     1741 ctgctgcccg tggccgtggt cgcctgctcg ggggagaaga aggccgccgc cgacccggcg
     1801 gcggccctgg ccgcggccaa gaccaccctc gacggcgccc aggccgtcac cctcgacctg
     1861 aagtccgaca agcccatccc gtccgggcac aacggggtgt cggctgccaa gggcaccgga
     1921 ctgatcgacc cgacgacgcc gaagttccaa ggcaccgtca gcgcggtcgt cagcggcgca
     1981 cccgtgacca ccgacgtcat caccatcggc gacacgacgt ggatgaagct gttcaccccc
     2041 accttcacca aggtcgacct caaggccctc aacgccccca accccggcag cttcttcaag
     2101 cccgacaccg gcctgtcaca gatcctcgcc aagacgagcg acgccgccgg aggcacggag
     2161 aaacgcttcg gcaaggaggt gctcacggct tacaccggga aggtccccgg cgccgtcgtc
     2221 aagagcctgc tcaacctcgg cgccgacacc tccacctacg ctgccgagtt cggcatcgcc
     2281 gagggcaacc agctgcgcac cgccacactg accggcccgt tctacgacac gggcaactcg
     2341 agctacgaga tcgtcctgac cgactacggc aagaccgtcg agatcgccca gccctgacgt
     2401 cgtgactgcc cccgcgcccg ccgcccgggc ccgcccgcgc gcgctgctcg ggtgggccgc
     2461 cgtcgcggtc gcgctcgccg ccgccgacac ctatgtcgtg gtcctcgccc tgacggagat
     2521 gatgcagggc gtcggtctcg gcatcgagaa cctccagcgc gccgccccga tcatcagcgg
     2581 cttcctgctc gggtatgtcg ccgtcctccc gctcatcggg cgtctcgccg atctcgtcga
     2641 tcgccagcgc gtgctcatgt ggtgcctggc gatcttcacg atcggcagcg gcctcaccgc
     2701 cctcgctgtc gaattgcccg tcctcgtcgc cggccgcgtg atccaggggt tgggtggggg
     2761 cgggctggtg ccggcgacgc ttgccatcgt cgccgcgtcc tggccgccgc acgagcgagg
     2821 tatgccgctc ggtctcgtcg gtgccgtcca ggagctcggg tcggtggtcg ggccagtgct
     2881 cggcgctgcc gtcctggccg ttgccgactg gcggatgatc ttctggctca acgcgatcgc
     2941 cggcatcgtg ctggcggcgg tgatccgccg cggcggccgg gcaaccgccg ggccgggcga
     3001 gccgccatcg ccccgcgccg gcgggcgcca gggctgggcg ctgcccgtcg tatggctgct
     3061 cgccctcgcg acactgtggc tagcgctcgc cgccccggac cggctcgcca ccgatgtcac
     3121 gttcggggtg cccttcgtgc ccttcgggtc gacgatctcc cgcctgacga ccccggtggg
     3181 tgccgcagca ctgagcctgg tgacgctcgc gctcgcctgg tcgctccgcc gggcctggcc
     3241 gatcctgcac caggccggtc cattcggggc ggccatgctc gccggcgcgc tcggcagcgt
     3301 catcctcacc ttcgcggccg cgcagccgga gaaggagatc gtcgggccac tcggctatgc
     3361 cctgctgccc gtcgcggcgg tctgcggcgc ggcatacgtg ctgcaccacc ggcgcgcgga
     3421 gaatcccgtg atcccgcacg gcgtggtgcg cggccgtacg gcctgggcgc tggtcgtctc
     3481 cttctgcgtc ggcgcggccc tggtggcggt catcgtcaac atccccctgc tggcgcgggt
     3541 ggccaatgcg gccgacgaga cagcggccgc gttcgtgctg ctgcgcttcc tcctggccgt
     3601 tccggtcggc gcgctgctgg gcggctggtc gctgcgccgt ctcggcgacg gcgtcgtctc
     3661 cgccaccggg ctggcgctcg cgacgctcgg gctgggtctg atgaccacct ggggccgcgg
     3721 cgtcttcgac accgccggag cgtggatcgt gttggcgctg agcggtttcg gtatcggcct
     3781 tgcgctcgcg ccggtcaaca acgctgcgct ggcggccgtg cgcgaggagg cccacggcgc
     3841 ggcgagcgcg ctcgtcgtcg tcgcccggat ggtcggcatg gtcctgggcc tggccctcct
     3901 gacggccatc ggcctgcacc gctacttcgc catcgtcgcg gtccttcccg acccgacgga
     3961 caaggaagcc ctcaaagatg ccgccatcgc ccaggtgcat tgggtcttcg ccggtgccgc
     4021 cgtggccgcc gccctcggcg ctctggcctc cctgcgcctc ggcctgcgcc g
//
LOCUS       DAOPJG010000247         4043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_24470_length_4043_cov_8.491474, whole genome shotgun sequence.
ACCESSION   DAOPJG010000247 DAOPJG010000000
VERSION     DAOPJG010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24470_length_4043_cov_8.491474"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKU75_15650"
     CDS             complement(<1..199)
                     /locus_tag="PKU75_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82360.1"
                     /translation="MLVTGAAVQITVEDHVVADIEMADLAVGDRLPVRVQQGEPGAER
                     GRAGGVGVAPEVFGRRDGRPGH"
     gene            609..1238
                     /gene="tmk"
                     /locus_tag="PKU75_15655"
     CDS             609..1238
                     /gene="tmk"
                     /locus_tag="PKU75_15655"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPF82361.1"
                     /translation="MSDGLFLAFEGGDGAGKSTQVRLLADVVAAGGREVVVTRQPGGT
                     ALGQQLRQLVLYGEAMAPRAEALIFAADKAQHVEEVIRPALARGAVVITDRYTDSAVA
                     YQGAGRELGSDEVHDLQMWAVGGLVPDLTVIIDLPPEEGRRRRGDVHDRLESEADAYH
                     AAVREHFLAIAAAAPERYLVIDGTQPPQALHAQIVGRLAAAGVVPGGAG"
     gene            1235..2374
                     /locus_tag="PKU75_15660"
     CDS             1235..2374
                     /locus_tag="PKU75_15660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPF82362.1"
                     /translation="MTVWREVIGQEPALAILQRAVAHPESMTHAWLFTGPPGSGRSVA
                     ARAFAGALMCPTGGCGECRECRTAVDGTHADVDLVATEGLTIGVGSARELVQLAARRP
                     SVGSWRVIIVEDADRLTEHSGNALLKAIEEPGSRTIWLLCAPSVEDVLITLRSRSRHV
                     RLRTPPVEAVAALLTDRDGVDPAMARYAARAAQCHVGMARRLARDERARIRRRQVVTM
                     PTRITGVGDAVGAAADLMAIAEEEKEHSLTERHTAERGRLLQLLGADPAARTQPPHVR
                     SQLAAMEKEQKTRATRFIRDVLDRTLLDLLSVYRDALVMRTGAPVAIVNEDNLDTVGG
                     LARALTPEGLLRAMEAIGTARERIDANVAPLLALEGMAVSLILPR"
     gene            2451..4013
                     /locus_tag="PKU75_15665"
     CDS             2451..4013
                     /locus_tag="PKU75_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF82363.1"
                     /translation="MSHPAAPRRPRAIGVLLAGSLALTGCSIDNPFSRDAKVTSTPLP
                     STSKVPTGQDALARFYNQTLAWTDCGGPKCAELEVPIDYAKPDGETIKLKVLKTEKRG
                     GGTKAGSILVNPGGPGGSGVEYARYADFIVGTPVRKAYDVVGFDPRGVQDSAPIECVE
                     PPQLDDFLGQDPTPDDAAERHQAVTIATDFGKACAANAGPLLGHVSTVEAAKDMDILR
                     AVVADAKLNYLGKSYGTYLGATYAGLFPDRVGRMVLDGALAPDLTPEETAIGQARGFD
                     TATRAWAADCVKEGNCPLGTSVDEVMTGLSDLLTKLDKTPATVTGDARVKKLTEGWAA
                     YGIAAAMYDQGMWAQLTDALRKVVASDDGTGLMNLADAYSERDASGAYSGNMMQVIYA
                     VNCLDAPDSPDIATYVEREKKAKEQAQLFGRFLAWSGLTCGHWPITSTNKPAKITAAG
                     SAPIVVVGTTRDPATPYEWAVRLADQLEAGVLVTMDGDGHTAYMRANSCIDNALNDFY
                     TKDKVPADGLRC"
BASE COUNT          593 a         1505 c         1381 g          564 t
ORIGIN      
        1 ggtgtcctgg gcgaccatcg cgacgcccga acacctccgg cgcgaccccg acaccgccgg
       61 cacgcccccg ctcggcaccc ggctcgccct gctggacgcg gaccggcagc cggtccccga
      121 cggcgagatc ggccatttct atgtcggcaa cgacatggtc ttcgacggtt atctggacgg
      181 cagcacccgt gaccagcacg ccggggtgat cgcgatcggt gacctcggct ggcgcagaat
      241 gggctgtatt tcgttgcggg acgggccgat cagctcatcg tctccggtgg cgagaatgtc
      301 catccgcagg aactggagga cgcgctgcat accgtgcccg gcgtcgccga ggcggccgcg
      361 ctcgcggagc cccgctgacc cgggtcctcg tgggtcggct cgaacttttc cgcccagtgc
      421 cacttcgtgt cggttcgcct cgcaacattc gcgatgcgaa ccgctacctt cccgcccaaa
      481 gctgtcgctc ccgccgagcc ctgagcggct cccgcccgac ctgacgctcc cgccgagccc
      541 tgacgctctg cggtgggctg cctgcggggc cggtgccggc tgtcataccc cgcggatagg
      601 gttcgcacgt gagtgacggg ctcttcctgg ccttcgaggg cggtgacggc gcgggcaagt
      661 cgacgcaggt gcgactgctc gccgatgtcg tcgcggccgg ggggcgcgag gtggtggtga
      721 cccggcagcc cgggggcacc gctctcgggc agcagttgcg gcaactggtc ctgtacggcg
      781 aggccatggc gccccgggcg gaggcgttga tcttcgccgc cgacaaggcc cagcacgtcg
      841 aggaggtgat ccgcccggcg ctggcgcggg gggcggtggt catcaccgac cgctacaccg
      901 actctgccgt cgcttatcag ggggccgggc gcgagctcgg cagcgatgag gtgcacgacc
      961 tgcagatgtg ggcggtcggc gggctcgtgc ccgacctgac cgtgatcatc gacctgccgc
     1021 ccgaggaggg gcgccggcgc cgcggcgacg tccacgaccg gctcgagtcg gaggccgacg
     1081 cctatcacgc cgccgtgcgc gagcacttcc tcgccatcgc cgccgctgcc cccgagcgct
     1141 atctggtcat cgacggcacg cagccgccgc aggccctgca cgcgcagatc gtgggccgcc
     1201 tggccgccgc cggcgtcgtc ccgggcggtg ccggatgacc gtatggcgtg aggtcatcgg
     1261 ccaggagccg gccctggcga tcctgcagcg ggccgtcgcc caccccgagt cgatgacgca
     1321 cgcgtggttg ttcaccggcc cgccggggtc ggggcgctcc gtcgccgcgc gcgccttcgc
     1381 cggggccttg atgtgtccca ccggcgggtg cggggagtgc cgcgaatgcc gcaccgccgt
     1441 cgacggcacc cacgccgacg tcgacctcgt cgcgaccgaa ggcctgacga tcggggtggg
     1501 ctcggcccgc gagctcgtgc agctggccgc tcgtcggccc tcggtcgggt cgtggcgcgt
     1561 catcatcgtc gaggacgccg accgcctcac cgagcactcc ggcaacgccc tgctcaaagc
     1621 gatcgaagaa cccggctcgc gcacgatctg gctgctctgc gccccgtcgg tcgaggacgt
     1681 gctgatcacc ttgcgctcgc gctcgcggca cgtgcggctg cgcaccccgc ccgtcgaggc
     1741 cgtcgccgcc ctgctcaccg accgcgacgg cgtcgacccc gcgatggcac ggtatgccgc
     1801 ccgcgccgcc caatgccacg tcggcatggc gcggcggctg gcgcgggacg agcgcgcgcg
     1861 gatccggcgc cgccaggtcg tcacgatgcc gacccggatc accggggtcg gcgatgccgt
     1921 cggcgccgcc gccgatctga tggcgatcgc ggaggaggag aaagaacatt ccctgaccga
     1981 gcggcatacg gcggagcggg ggcgactgct ccaactgctc ggcgccgacc cggccgcccg
     2041 cacccagccg ccacacgtgc gcagccagct ggcggcgatg gagaaggaac agaagacccg
     2101 cgccacccgc ttcatccgcg acgtcctcga ccgcaccctg ctcgacctgt tgtcggtcta
     2161 ccgcgacgcc ctggtcatgc ggaccggcgc cccggtcgcc atcgtcaacg aggacaatct
     2221 cgacaccgtc ggcgggctgg ctcgggccct gaccccggag ggtctgttgc gcgcgatgga
     2281 ggccatcggc accgcccgcg aacggatcga cgccaatgtc gccccgttgc tggcgctcga
     2341 gggtatggcc gtctcgctca tcctgccgcg gtgaccccgc gccgggatcg ttatcgcggt
     2401 gcgatgaact cagtgcgcgt cctgtcggat gcgtgagtta gcgtcgagcc atgtcgcatc
     2461 cggccgctcc ccgtcgtccg cgcgcgatcg gggtgttgct cgccggatcg ctcgccctga
     2521 ccggttgctc gatcgacaac cccttctccc gcgacgcgaa ggtcaccagc accccgctgc
     2581 cgagcacgtc caaggtgccg acggggcagg acgcgctggc caggttctac aaccagacgc
     2641 tggcctggac cgactgcggc gggccgaagt gcgccgagct ggaggtgccg atcgactacg
     2701 cgaagccgga cggggagacg atcaagctca aggtcctcaa gaccgagaaa cgcggcggcg
     2761 gcaccaaggc cggttcgatc ctcgtcaacc ccggcgggcc cggcggcagc ggcgtcgagt
     2821 atgcccgtta tgccgacttc atcgtgggca cgccggtccg caaggcctac gacgtcgtcg
     2881 gtttcgaccc gcgcggggtg caggactcgg ccccgatcga gtgcgtcgaa ccgccgcagc
     2941 tcgacgactt cctcggccag gaccccaccc cggacgacgc agcggagcgg catcaggccg
     3001 tgaccatcgc gaccgacttc ggcaaggcgt gcgccgccaa tgcggggccg ctgctcgggc
     3061 acgtctcgac cgtcgaggcg gccaaggaca tggacatcct gcgggcagtc gtcgccgatg
     3121 cgaagctgaa ctacttgggc aagtcctacg gcacctacct cggcgccacc tatgccggcc
     3181 tcttccccga ccgagtcggg cggatggtcc tcgacggcgc cctcgccccc gacctgaccc
     3241 ccgaggagac ggccatcggc caggcccgcg gattcgacac cgccacccgc gcctgggcgg
     3301 ccgattgcgt caaggagggc aactgccccc tcggcacctc ggtcgatgag gtgatgaccg
     3361 ggctcagtga cctgctcacc aagctggaca agacgcccgc gacagtcacc ggcgacgccc
     3421 gcgtcaagaa gctgacggag gggtgggcgg catacggcat cgccgcggcc atgtacgacc
     3481 aaggcatgtg ggcccagctg accgacgccc tgcgcaaggt cgtggcgtcc gacgacggca
     3541 ccggcctgat gaacctcgcc gacgcctact ccgagcggga cgcatcgggc gcctactcgg
     3601 gcaacatgat gcaggtcatc tatgccgtca actgcctcga cgcccccgac agccccgaca
     3661 tcgcgaccta tgtcgagcgc gagaagaagg ccaaggaaca ggcccagctc ttcggccgct
     3721 tcctggcctg gtcgggcctg acctgcggcc actggcccat cacgtcgacg aacaaaccgg
     3781 ccaagatcac cgccgccggc tccgccccga tcgtcgtcgt cggcaccacc cgcgaccccg
     3841 ccactcccta cgagtgggcg gtccggctcg ccgaccagct cgaggccggc gtcctggtga
     3901 ccatggacgg ggacggccac acggcataca tgcgggccaa ctcgtgcatc gacaacgccc
     3961 tcaacgactt ctacaccaag gacaaggtcc cggccgacgg cctgcgctgc tgacggtccc
     4021 ggtcagcgga tgctgctgac ccc
//
LOCUS       DAOPJG010000248         4038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_24506_length_4038_cov_9.140346, whole genome shotgun sequence.
ACCESSION   DAOPJG010000248 DAOPJG010000000
VERSION     DAOPJG010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24506_length_4038_cov_9.140346"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PKU75_15670"
     CDS             complement(<1..142)
                     /locus_tag="PKU75_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HPF82364.1"
                     /translation="METAEIRRRWLSFFESKGHLVVPSASLILNDPNLLFVNAGMVPF
                     KPY"
     gene            complement(322..597)
                     /locus_tag="PKU75_15675"
     CDS             complement(322..597)
                     /locus_tag="PKU75_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82365.1"
                     /translation="MTGRPLTLGLAALAGGAAAYGLYRFGGRYRPSDPVAGLGDFVRQ
                     VRGGMAERERELRDALGYEAAANQPGGARLSDERARDLIRDPAGFDL"
     gene            complement(594..983)
                     /locus_tag="PKU75_15680"
     CDS             complement(594..983)
                     /locus_tag="PKU75_15680"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HPF82366.1"
                     /translation="MFENVSLGDIALLILALAVSFLVYRLGSLIGKAGRILDETQASL
                     KVTTDNVQPTLVKLTDTVSLTNDQLARVDSITANVGSMATNANALTSLFAATVGQPLV
                     KAAAFSYGVRSALAKPAGGRLPQQDES"
     gene            1091..2107
                     /locus_tag="PKU75_15685"
     CDS             1091..2107
                     /locus_tag="PKU75_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPF82367.1"
                     /translation="MIDLATVARPIIGAPMAGGPSTPTLVEAVGAAGGFGMLAAGYLT
                     PAALADAMAATRGPFGVNLFCPPDTEPDLASAQAYRESLAPLAQQYAVELPPVAVAAA
                     ADDDHWAEKVDLLCERPPAAVSFTFGLPDAETVERLHARDCTLLATVASVADARAAAA
                     LGVDAMCVQGPEAGGHRATRRIADEPEDVPLLELIAAVRAAVSVPVIAAGGLMDAAAV
                     EAVLKAGAVAAQVGTALLLADEAGTSATHRAALTDPQFTETAVTRAFTGRPARGLRND
                     FVERYDTGAPAAYPAVHQLTRPIRAAAAAARNPAHVHLWAGTGWHHARPAPAADIITR
                     LTPA"
     gene            complement(2274..3074)
                     /locus_tag="PKU75_15690"
     CDS             complement(2274..3074)
                     /locus_tag="PKU75_15690"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPF82368.1"
                     /translation="MNMQIARRATVVAMAAGLGITAAAPALAETTVPAPKELANAAVG
                     PTAPVPQEARRGESDPVVKRAQQIMAKFGIPAGPADGLTGQQTRQGLCAFRRISGLPV
                     NRYSLNTETYRKLVEYDNKYSRLNQIGVSSFQGKSTFVHVQQACQTMFYVKDKKVQRV
                     LPVSTGVRGHETPFGYYTLANTTRGWYCSTLYPESCRKQTTGEFAYISNFGNMYNPRQ
                     VVGAIFVHGSTSVPTYPASHGCIRVTVNDSDWLYHNVDRMPIFITGKY"
     gene            complement(3192..>4038)
                     /locus_tag="PKU75_15695"
     CDS             complement(3192..>4038)
                     /locus_tag="PKU75_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPF82369.1"
                     /translation="SLSDNEIRDVVEAALTAPEGLGAAYTLDDDAREHIVRVSGGDAR
                     RALTALEAAAGVAEDLRGIRGDDQPTTISLPAVEQAMAHAAVRYDRAGDQHYDVASAL
                     IKSMRGSDVDAALHYLARMLEAGEDPRFIARRIVIAASEDVGMGDPTALQTAVAALHA
                     VAQIGMPEARIILAQAVVHNALAPKSNASYLAINAAIADVRAGKGGAVPAHLRGSGYA
                     GAARLGHGDGYRYAHDEPDGVAEQQYLPDDLQGTTDYYRPTDRGFEARLQERWKWLRS
                     RLGRP"
BASE COUNT          590 a         1345 c         1468 g          635 t
ORIGIN      
        1 agtagggctt gaagggcacc atgccggcgt tgacgaagag caggttgggg tcgttgagga
       61 tcaacgaggc cgacggcacc acgaggtggc ccttgctctc gaagaaggac agccagcggc
      121 gccggatctc agcggtttcc atgggtgatc gacctaggtg ctcgggttcg tgcgggacag
      181 tgcagtctaa ttgccttcac gttcctctcc tcatgcaatc agggcgccct gcgcgttcct
      241 cgcagcgcga cctcctcgca tcgtcgacga accggagtcc ggttcgcgag caggctgact
      301 tggctgccgt ctccccgtgg ctcagaggtc gaatcccgcc gggtcgcgga tcaggtcgcg
      361 tgcccgctcg tcgctcaggc gtgctccccc gggttggttg gccgccgcct cgtagcccaa
      421 ggcgtcgcgc agctcgcgtt cgcgctcggc catgccgccg cgcacctgcc ggacgaagtc
      481 gccgagtccg gcgacgggat cactcggacg ataacggccg ccgaagcgat acaggccgta
      541 ggcggcggcc ccgcccgcga gcgccgcgag gccgagcgtg agtggccggc cggtcacgac
      601 tcgtcctgtt gcgggaggcg cccgccggcc ggcttcgcca acgccgagcg caccccatag
      661 gagaaggcag cggccttgac cagcggctgc ccgaccgtcg cggcgaagag ggaggtgagc
      721 gcgttggcgt tggtggccat cgacccgacg ttggcggtga tggagtcgac ccgcgcgagt
      781 tggtcgttgg tcagcgacac ggtgtcggtg agcttgacca gggtcggctg cacattgtcg
      841 gtcgtcacct tcagcgacgc ctgtgtctcg tcgaggatcc gccccgcctt gccgatgagc
      901 gacccgagcc gatagacaag gaaggagacg gccagcgcca ggatcagcag ggcgatatcg
      961 ccgagcgaga cattctcgaa catgccggca agactaatcc cgccgcgcag cctgccctcg
     1021 cgagcttcgg tgtcgccaga gggccgcatc gtgcttcccg accagagcgg ccgatcccta
     1081 atctggagcg atgatcgacc tcgcgaccgt ggcccggccc atcatcggcg caccgatggc
     1141 gggcggaccg tcgacgccca ccttggtgga ggcggtgggc gcggccggtg ggttcgggat
     1201 gctggccgcg ggttacctga cgcccgccgc gctggcggac gcgatggccg cgacccgcgg
     1261 cccgttcggg gtcaatctct tctgcccgcc ggacacggaa ccggatctgg cttcggcgca
     1321 ggcgtatcgc gagagcctgg ccccgctggc gcagcagtat gccgtcgagc tcccgcccgt
     1381 agctgttgcc gccgcagccg acgacgacca ctgggccgag aaggtcgacc tgctctgcga
     1441 gcgtccaccc gcggcggtct ccttcacgtt cgggctgccc gatgccgaga ccgtcgagcg
     1501 cctgcacgcg cgagactgca ccctcctcgc gacggtggcg tcggtggcgg acgcccgggc
     1561 cgctgcggcg ctgggggtgg atgcgatgtg cgtgcagggt cccgaggccg gtggtcaccg
     1621 ggccacccgg cgcatcgccg acgaacccga ggacgtgccg ttgctcgagc tgatcgcggc
     1681 ggtccgggcc gcggtgtcag tgccggtgat cgcggccggt gggctcatgg acgccgcggc
     1741 cgtcgaggcg gtgctgaaag cgggagccgt tgcggcacaa gtaggtacgg ccctgctcct
     1801 ggcagacgag gccggcacct cggcgaccca tcgggcggcg ctgaccgacc cgcagttcac
     1861 ggagacagcg gtgacgcgcg ccttcaccgg tcgcccggcc cgcggcctgc gcaacgactt
     1921 cgtggagcgt tacgacaccg gtgcgcccgc ggcatacccg gcagtgcacc agctcacccg
     1981 ccccattcgc gccgccgcgg cagccgcgcg aaaccccgcc cacgtccacc tctgggccgg
     2041 caccggctgg caccacgcgc gtccggcgcc ggccgccgac atcatcaccc gcctgacgcc
     2101 ggcgtagtcg ccgaaccgtc gagcgaagcg agttcctagc cttccgggga ctggcagcgg
     2161 gaggcgaggc cagaattccg cgcgagcgga ccggggaacc gtcgagcgca gcgagcttgg
     2221 ttcaccggaa cggaaaacca gcgcgagcgc cagcgagctc ctggccttac gggtcagtac
     2281 ttgccggtga tgaagatcgg catgcggtcg acgttgtggt agagccagtc ggagtcgttg
     2341 acggtgaccc ggatgcagcc gtgcgaggcg gggtaggtcg gcaccgacgt ggagccgtgg
     2401 acgaagatgg ccccgacgac ctggcgtggg ttgtacatgt tcccgaagtt ggagatatag
     2461 gcgaactcgc cggtggtctg cttgcggcac gactcggggt agagcgtgga gcagtaccag
     2521 ccgcgcgtgg tgttggccag cgtgtagtaa ccgaaggggg tctcgtggcc ccggaccccc
     2581 gtcgagacgg gcaggactcg ctggaccttc ttgtccttga cgtagaacat cgtctggcac
     2641 gcctgctgaa cgtgcacgaa ggtcgacttg ccctggaagg aggagacccc gatctggttg
     2701 agccgggagt atttgttgtc gtattcgacg agcttgcgat aggtctcggt gttgaggctg
     2761 tagcggttga cgggcaaccc ggagatgcgc cggaaggcgc acagcccctg gcgggtctgc
     2821 tgaccggtga ggccgtcggc cgggccggcg gggatgccga acttggccat gatctgctgg
     2881 gcacgcttga cgaccgggtc ggattcgccg cggcgcgctt cctggggcac cggcgcggtc
     2941 gggcccacag cggcgttggc caactccttg ggcgcgggga ccgtggtctc ggctaaagcc
     3001 ggtgcggcag cggtgattcc gaggcccgcg gccatggcga caacggtggc gcggcgtgcg
     3061 atctgcatgt tcatggtgat tccctctccc tatggacccg atggcgggtg cagcgagctt
     3121 cagcgtcgct gtcatcacct cagacgcctc agggcactca taggttgtgc aaagacaaga
     3181 ttacggtctg atcacggccg gccgagccgc gagcgcaacc atttccaccg ctcctgcagg
     3241 cgggcctcga atccccggtc ggtgggccgg tagtagtcgg tcgtgccttg caagtcgtcc
     3301 gggaggtact gctgctccgc cacgccgtcg ggctcgtcgt gggcgtagcg atagccgtcg
     3361 ccgtgaccga ggcgggcagc gccggcatac ccgctgccgc gcaggtgcgc gggcaccgcg
     3421 ccgcccttgc ccgcccgcac gtcggcgatc gccgcgttga tcgcgagata ggaggcgttc
     3481 gacttcgggg cgagcgcatt gtgaacgacg gcctgcgcca ggatgattcg cgcttcgggc
     3541 atcccgatct gggcgacggc gtgcagcgcc gcgacggctg tctggagggc ggtggggtcg
     3601 cccatgccga cgtcctcgga ggcggcgatg acgatgcggc gggcgatgaa gcgcggatcc
     3661 tcacccgcct ccagcatgcg cgcgaggtaa tgcagcgctg cgtcgacgtc ggaaccccgc
     3721 atcgacttga tcagggccga ggcgacgtcg tagtgctggt cgccggcgcg gtcgtagcgc
     3781 acggccgcgt gagccatcgc ctgctcgacc gccggcagcg agatcgtggt cggctgatcg
     3841 tcgccccgta tgccgcgcag atcctccgcg acgccggccg cagcttcgag ggcggtcagg
     3901 gcgcggcgcg cgtcaccgcc gctgacgcgc acaatgtgct ctctcgcgtc gtcgtcgagc
     3961 gtgtatgccg cgcccaggcc ttccggcgcg gtgagtgcgg cctcgacgac atcgcgaatc
     4021 tcgttgtccg acagggat
//
LOCUS       DAOPJG010000249         3994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_24867_length_3994_cov_8.735720, whole genome shotgun sequence.
ACCESSION   DAOPJG010000249 DAOPJG010000000
VERSION     DAOPJG010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24867_length_3994_cov_8.735720"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..>1180)
                     /locus_tag="PKU75_15700"
     CDS             complement(23..>1180)
                     /locus_tag="PKU75_15700"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPF82370.1"
                     /translation="MHVVIAPDCFTGTLTATQAAEAMAAGWAQTAPGDVLTLVPLSDG
                     GPGFLDVLERALDGEMIATIVGDPLGREVPAGVLLVGAEGERTAYVESAQAAGLHLLA
                     ASERDPAVTTSYGVGQLLLAAREAGAKRVVVGLGGSGTNDGGAGLLAALGAGPPEHLA
                     RGGLALRDLGPDALAGLDRVQAEWAGIEIVAATDVDNPLLGLQGASAVFGPQKGASPQ
                     LAQDLEGALGHYADRVWRFGPPRTDLLTGTPRRLDKEPGAGAAGGLGFALFVLGAHRR
                     SGVDLVLDAVGFRDLCAAADLVVTGEGSFDWQSLRGKVVTGVAQRALATATPAIVIAG
                     RVLVGRREAMGVGLSGMYAVAADPAQEEAAFADPVGTLRARAARVARTWSP"
     assembly_gap    1181..1280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1325..2518
                     /gene="nadA"
                     /locus_tag="PKU75_15705"
     CDS             1325..2518
                     /gene="nadA"
                     /locus_tag="PKU75_15705"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPF82371.1"
                     /translation="MTTTPLPLLVLGKGRDLHTERGVECPGDLPPASDPALVERARAA
                     KEALGDRVFILGHHYQRDEIIEFADTTGDSFKLAREAAARPEAEYIVFCGVHFMAESA
                     DILTGPDQTVILPDLAAGCSMADMAAISQVEDAWQDLQDAGVADATLPVTYMNSSAAI
                     KAFTGRLGGTICTSSNAKTALTWAFEQRGDGQILGGTGKVLFLPDQHLGRNTYVRELG
                     GSLEECVVFDPHKPMGGLSVEELRAARMILWRGHCSVHGRFSVDAVETARREIPDVNV
                     IVHPECQYEVVELADYVGSTEKIIKTIAAAPDGSSWVVGTELNLVQRLAKQFPGKRIA
                     FLEKNVCYCSTMNRIDLPHLVWALESLVEGRVVNPIRVEPEVAGYARAALDRMLALPG
                     ETHND"
     gene            complement(2710..>3994)
                     /locus_tag="PKU75_15710"
     CDS             complement(2710..>3994)
                     /locus_tag="PKU75_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPF82372.1"
                     /translation="LEALTRIPSVSLDAFDQAEVERSAEATAALFTAEGFDAEIVRAG
                     GRPAVIARKEGPAGAPTVLLYAHHDVQPPGSEELWESSPFEPTERDGRLYGRGAADDK
                     AGIMAHLAAVRAHGDDLPVTVVVFVEGEEEIGSESLPATLAAYGDRLQADAIVLADST
                     NWAIGEPALTTTLRGLIRAVVTVSTLSHGIHSGMFGGAVPDALTALIKLLATLHTDDG
                     EVAVTGLAADSASELPYDEPRLRAESGLLDGVELIGRGPLLERIWAKPSLTTIGIDAP
                     TVDTASNTLVPSARAKISMRIAPSQEPMEAFEILKDFLLDRAPWGAHVEVALDDQGAG
                     FAADATGPMYDAARAAFADAWGHPPVDIGVGGSIPFVAAFAERFPDAALLVTGVEDPD
                     TRAHGANESLHLGEFEKVCLAEAALLARIAALPRP"
BASE COUNT          561 a         1385 c         1429 g          519 t
ORIGIN      
        1 aaccaactcc ggggcaacca cgtcagggcg accaggtccg ggcgacgcgc gccgcccgcg
       61 cccgcagcgt cccgaccggg tcggcgaagg cggcctcctc ctgggcggga tcggcggcga
      121 cggcatacat tccgctcagg cccaccccca tcgcctcgcg ccggccgacg aggacccgtc
      181 cggcgatcac gatcgcgggc gtggcggtgg cgagcgcgcg ctgagcgacc ccggtgacga
      241 ccttgccgcg caggctctgc cagtcgaagg acccctcccc cgtcacgacg aggtcggcgg
      301 cggcgcacag gtcgcggaat ccgacggcgt cgaggacgag gtcgacgccg ctgcggcggt
      361 gtgcaccgag gacgaagagc gcgaatccca gcccgccggc cgcgcccgcc ccgggttcct
      421 tgtccagccg acgcggggta ccggtgagca ggtcggtgcg gggcggcccg aaccgccaga
      481 cccggtcggc gtagtgcccg agggcgccct cgaggtcctg cgcgagttgc ggggaggcgc
      541 ccttctgcgg accgaagacc gcgctcgcgc cctgcagccc gagcaggggg ttgtcgacat
      601 cggtggcggc gacgatctcg atgccggccc actcggcctg cacccggtcc agcccggcca
      661 gcgcgtcggg gccgaggtcg cgaagggcca ggccgccgcg ggccaggtgc tcgggcggac
      721 cggcaccgag ggcggcgagc aggcccgccc cgccgtcgtt ggtcccgctg ccgcccaacc
      781 cgacgacgac ccgcttggcc cccgcctcgc gggcggcgag gaggagctga cccacgccat
      841 acgacgtggt caccgccggg tcccgctcgc tggcggcgag caggtgcagc cccgccgcct
      901 gcgccgattc aacgtatgcc gtccgctccc cctccgcgcc cacgaggagc actcccgccg
      961 gcacctctcg cccgagcgga tcgccgacga tagtcgcgat catctccccg tccagcgcgc
     1021 gctcgaggac gtcgaggaag cccggccccc cgtcggacag cgggaccagc gtcagcacat
     1081 cgcccggcgc cgtctgcgcc cacccggccg ccattgcctc cgcggcctgc gtggccgtca
     1141 gggtgccggt gaagcagtcc ggggcgatga cgacgtgcat nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn gcgacccaac cccgttccgc ggccacccgg cataccatcg
     1321 gcgaatgacg acgacaccgc tgccgctgct agtcctcggg aagggccgcg acctccatac
     1381 cgaacgcggc gtcgagtgtc ccggcgacct gccgccggca tccgaccccg ccctggtgga
     1441 gcgcgcgcgg gcggcgaagg aggcgctcgg ggaccgggtg ttcatcctcg gtcaccacta
     1501 ccagcgcgac gagatcatcg agttcgcgga caccaccggc gactctttca agctcgcgcg
     1561 ggaggccgcg gcccggccgg aggccgaata catcgtcttc tgcggcgtgc acttcatggc
     1621 cgagtcggcc gacatcctga ccggccccga ccagacggtg atcctgcccg acctggcggc
     1681 cggctgctcg atggccgaca tggcggcgat cagccaggtc gaggacgcgt ggcaggacct
     1741 gcaggacgcg ggggtcgcgg acgccacgct gcccgtcacc tatatgaact cctccgccgc
     1801 catcaaggcc ttcaccggtc gcctcggtgg cacgatctgc acctcgtcca acgccaagac
     1861 ggcgctgacg tgggcgttcg agcagcgcgg cgacggccag atcctcggcg gcacgggcaa
     1921 ggtgctcttc ctgcccgacc agcacctggg acgcaatacg tatgtccgtg agctcggagg
     1981 atcgctcgag gagtgtgtgg tgttcgaccc gcacaagccg atgggcggcc tgagcgtcga
     2041 ggagttgcgt gcggcgcgga tgatcctgtg gcgcggccac tgctcggtgc acgggcgctt
     2101 cagtgtcgac gccgtcgaga ccgcgcgccg cgagatcccg gacgtcaacg tcatcgtcca
     2161 tcccgagtgt cagtacgagg tcgtagagct ggccgactac gtcggctcca ccgagaagat
     2221 catcaagacg atcgccgccg cgcccgacgg ctcgtcgtgg gtggtcggca ccgaactgaa
     2281 cctggtgcag cggctggcca agcagttccc cggcaagcgg atcgcgttct tggagaagaa
     2341 cgtctgctat tgctcgacga tgaaccgcat cgacctgccg cacctggtgt gggcgctgga
     2401 gtcgctcgtc gagggccggg tcgtcaaccc gatccgcgtc gaacccgagg tcgccggcta
     2461 tgcccgggcc gccctcgacc gcatgctcgc tcttcccggt gagacccaca acgactgagc
     2521 ccggcgacca ggagcgaccc ggcccgagtg agctggggtc gggtactttt ccgcccagtg
     2581 ccacttcctt gcggttcgcc tcgcaacatt tccgttgcga accgatacga agtggcactg
     2641 ggcggaaaag ttcacgcgcc acgatgagcc ctggcgacca ggagcggccc gctcgaccgg
     2701 gccgagcggc tacggccgcg ggagggcggc gatgcgggcg agcagggcgg cctcggcgag
     2761 gcaaaccttc tcgaactcgc cgaggtgcag cgactcgttg gcgccgtggg cgcgtgtgtc
     2821 cgggtcctcg acaccggtga cgaggagggc ggcatcgggg aaccgctccg cgaaggcggc
     2881 gacgaagggg atggagccgc cgacgccgat gtcgacgggc gggtgacccc acgcgtcggc
     2941 gaaggcggcg cgggcggcgt cgtacatcgg gccggtggcg tcggcggcga agccggcgcc
     3001 ctggtcgtcg agggcgacct cgacgtgcgc accccacggg gcacggtcga ggaggaagtc
     3061 cttgaggatc tcgaaggcct ccatcggctc ttgggagggc gcgatgcgca tggagatctt
     3121 ggcccgggcg ctcggcacga gcgtgttgga cgcggtgtcg acggtcgggg cgtcgatgcc
     3181 gatcgtcgtc agcgacggtt tggcccagat gcgctcgagc agcggcccgc ggccgatcag
     3241 ctcgaccccg tcgagcagcc cggattcggc gcgcaggcgc ggctcgtcat acggcagttc
     3301 gctcgcggag tcggcggcga gcccggtgac ggccacctcg ccgtcgtcgg tatgcagtgt
     3361 ggccagcagc ttgatcagcg cggtcagcgc gtcgggcacc gcgccgccga acatgccgga
     3421 gtgaatcccg tgcgagaggg tcgagacggt gacgacggcg cggatcaggc cgcgcagggt
     3481 cgtggtcagc gcgggttcgc cgatcgccca gttggtcgag tcggcgagga cgatcgcgtc
     3541 ggcctgcaac cggtcgccat atgccgcgag ggtcgccggc agcgactcgg acccgatctc
     3601 ctcctccccc tcgacgaaga cgacgacggt gaccggcagg tcgtcgccgt gggcgcggac
     3661 ggcggccagg tgcgccatga tgccggcctt gtcgtcggca gcgccgcggc cgtagaggcg
     3721 gccgtcgcgt tcggtcggct cgaacggcga actctcccat agctcttcgg agccgggagg
     3781 ctggacgtcg tggtgggcat agagcagcac cgtcggggct ccggccgggc cctccttgcg
     3841 ggcgatgacg gcgggccgcc cgccggcgcg cacgatctcg gcgtcgaagc cctcggccgt
     3901 gaagagcgcc gcggtggcct cggcgctgcg ctcgacctcg gcctggtcga aggcgtcgag
     3961 cgagacgctc gggatgcggg tgagggcctc gagg
//
LOCUS       DAOPJG010000250         3974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_25055_length_3974_cov_7.292166, whole genome shotgun sequence.
ACCESSION   DAOPJG010000250 DAOPJG010000000
VERSION     DAOPJG010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25055_length_3974_cov_7.292166"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKU75_15715"
     CDS             complement(<1..383)
                     /locus_tag="PKU75_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPF82373.1"
                     /translation="MADTSDVTPAPAPSEQPEYDASAITVLEGLEAVRKRPGMYIGST
                     GERGLHHLVWEIVDNAVDEALAGYADTILVTLMADGSVRVKDNGRGIPTDIHPTEGVS
                     AVELVLTQLHAGGKFGGGGYKVSGGL"
     gene            complement(609..1085)
                     /locus_tag="PKU75_15720"
     CDS             complement(609..1085)
                     /locus_tag="PKU75_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82374.1"
                     /translation="MFRKLGYAALAAGSVAGLATAAPAQATTSAPAGNYCVGDIKGGL
                     IGCYDTEREADRVMANKRWIPLVIFFDNTGYSGKRLTLGHARPCTATLSDVNYALPDL
                     RVYRWNNRAGSFVTRNRCDLKGYDGFNYNGDRFRRWVDHDIRLTRWNNDISSFKLS"
     gene            complement(1225..3204)
                     /gene="thrS"
                     /locus_tag="PKU75_15725"
     CDS             complement(1225..3204)
                     /gene="thrS"
                     /locus_tag="PKU75_15725"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPF82375.1"
                     /translation="MSAQITVNIAGSERSVPEQTTAADLFGDDRAVLVARVNGELRDL
                     AHVLADGDTVEPVTAAEQDGLDVLRHSTAHVLAQAVQEVNPQAKLGIGPPIRDGFYYD
                     FDVETPFNPEDLKALEKVMQRIINEGQTFHRREISDADALAELADEPYKCELIGLKGG
                     AADDAAEGAGVEVGGAQLTIYDNLKKDGTRAWGDLCRGPHVPSTRVIGNAFKLMRSAA
                     AYWRGSEKNPSLQRVYGTAWPSKDALKAYLDRLAEAERRDHRKLGAELDLFSFPDELG
                     SGLPVFHPKGGIIKREMEDYVRRRHIEEGFEYVGTPHISKDGLFHTSGHLPYYADTMF
                     PPMEFEGSNYRLKAMNCPMHNLIYKSRGRSYRELPLRLFEFGHVYRYEKSGVIHGLTR
                     LRGFAQDDSHSYVTKEQAAAEIKHLLGFCLGLFRDFGLDDFYLELSTRDDSKKDKFIG
                     SEEQWAEATAILEQVCVESGLELVPDPGGAAYYGPKVSIQARDAIGRTWQMSTIQYDF
                     NQPERFGLEYQAADGTRQQPVMIHSAKFGSIERFLGVLVEHYAGAFPVWLSPVQVLGI
                     PVAEDFNDYLWDVLKQMKEQGIRVELDESDDRFPKKIRNAAKAKVPFVLIAGAEDQAK
                     NAVSFRYRDGSQENGVPIADAIAKVLAAIESKAQV"
     gene            complement(3448..>3974)
                     /locus_tag="PKU75_15730"
     CDS             complement(3448..>3974)
                     /locus_tag="PKU75_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82376.1"
                     /translation="VKSAWAQVGVLGGAESDGEGPVPVVDLPADPGEPVPAPVPDDSG
                     EPSPEWPGVPEEGGVGHHERGPDDMEHEGEVPAGAVVEVTRSGGVAGLTARRSVALDE
                     LPAPQQQQWRSVLGNRTLQTLDAQAAASAARQHPDTYCYGVSCPTPPTEVSIREQDLP
                     DDLKALLEETLREA"
BASE COUNT          565 a         1351 c         1384 g          674 t
ORIGIN      
        1 agaccgccgg agaccttgta gccgccgccg ccgaacttgc cgccggcgtg cagctgggtg
       61 agaaccagct caacggcgct gacgccctcg gtcgggtgga tgtcggtggg gatgccgcgg
      121 ccgttgtcct tgacccgcac cgacccgtcg gccatcagcg tgacgaggat cgtgtcggca
      181 taccccgcga gcgcctcgtc caccgcgttg tcgacgatct cccacaccag gtggtgcagg
      241 ccgcgctcgc cggtggagcc gatgtacatg ccggggcgct tgcgcaccgc ctcgagtcct
      301 tcgaggacgg tgatggcgct ggcgtcatac tccggctgct cgctgggcgc gggcgcaggc
      361 gtgacatcgg acgtgtcggc cacggtgctc cttcgggaga gaatgcgcag gtgccgaccg
      421 tgcgtcacgt cggcgggatc taccccgaaa ttctacccgc tcgcaccgac aatcaccgca
      481 ttcccggccc gtgcgggcct gctagcgcgt cgcactcccc caggtccgtg agggattacg
      541 cggcaccccc cgttctggcc cgtatgccgc cttcccgggg tccgcccgcc ggtcaccctc
      601 cgcgggcgtc agctgagctt gaaggaactg atgtcgttgt tccagcgggt caggcggatg
      661 tcgtggtcga cccagcggcg gaagcggtca ccgttgtagt tgaagccgtc gtagcccttc
      721 aggtcacaac ggttgcgggt gacgaacgaa ccggcgcggt tgttccagcg gtagacgcgc
      781 agatcgggca gggcatagtt cacgtccgac agggtcgcgg tgcagggacg cgcgtggccg
      841 agggtcagtc gcttgccgct gtagccggtg ttgtcgaaga agatgaccag cgggatccag
      901 cgcttgttgg ccatcacccg gtcggcttcc cgctcggtgt cgtagcagcc gatgagcccg
      961 cctttgatgt ccccgacgca gtagttgcct gccggcgccg acgtggttgc ctgcgccgga
     1021 gcggcggtgg cgagaccggc gacggaaccg gcggcgaggg cggcataccc cagtttgcgg
     1081 aacatggtgg gtcgtccttt cgtcgtgtgg cccggacccc cctggcccag gcacgccgcc
     1141 aaacctggca cgcgcacccc ccgtgacctg cggaaagggg ctggttgacc cgtaaactac
     1201 ccgaaagggt ggaccgcggc tcggctacac ctgggccttg gactcgatcg cggcgagcac
     1261 cttggcgatc gcgtccgcga tcgggacgcc gttctcctgg gagccgtcgc gatagcggaa
     1321 ggagaccgca ttcttggcct ggtcctcggc gccggcgatg agcacgaacg gcaccttggc
     1381 cttggcggca ttgcggatct tcttggggaa gcggtcgtcg gattcgtcga gttcgacgcg
     1441 gatcccctgc tccttcatct gcttcaggac atcccacagg tagtcgttga agtcctccgc
     1501 gaccggtatg ccgagcacct ggaccgggga gagccacacc gggaaggccc cggcatagtg
     1561 ctccaccagc accccgagga agcgctcgat cgagccgaac ttcgccgagt ggatcatcac
     1621 cggctgctgc cgcgtgccgt cggccgcctg atactccagg ccgaagcgct cgggctggtt
     1681 gaagtcgtac tggatcgtgc tcatctgcca ggtccggccg atagcgtcgc gggcctggat
     1741 cgagaccttc ggcccgtaat aggctgcgcc acccgggtcg ggcacgagct cgagcccgga
     1801 ctccacgcac acctgctcga ggatggccgt cgcctcggcc cactgctcct cggaaccgat
     1861 gaacttgtcc ttcttggagt catcgcgcgt cgacagctcc agatagaagt cgtccagccc
     1921 gaagtcgcgg aagagaccca ggcagaaacc gagcaggtgc ttgatctccg cggcggcctg
     1981 ctccttggtg acataggagt ggctgtcgtc ctgggcgaag ccgcgcagcc gcgtcaggcc
     2041 gtggatgacc ccggacttct cgtagcggta gacatgcccg aactcgaaga gccgcaacgg
     2101 cagctcgcgg taggagcgac cccgcgactt gtagatcagg ttgtgcatcg ggcagttcat
     2161 cgccttgagg cggtagttgc tgccctcgaa ctccatcggc gggaacatcg tgtccgcgta
     2221 atagggcagg tgcccgctgg tgtggaagag cccgtccttg gagatatgcg gcgtgccgac
     2281 atattcgaag ccctcttcga tatgccggcg gcggacgtag tcctccatct cccgcttgat
     2341 gatgccgccc ttggggtgga agaccggcag gccggagccg agttcgtcgg ggaaggagaa
     2401 gaggtcgagc tcggcgccga gcttgcggtg gtcgcgccgc tcggcctcgg cgagccggtc
     2461 caggtaggcc ttcagggcgt ccttgctcgg ccaggccgtc ccgtagaccc gctgcagtga
     2521 cggattcttc tcgctgccgc gccagtaggc ggcggcgctg cgcatcagct tgaacgcgtt
     2581 gccgatgacc ctcgtcgagg gcacgtgcgg cccgcggcac aggtcgcccc aggcgcgggt
     2641 gccgtccttc ttgaggttgt cgtagatcgt cagctgcgcc ccgccgacct cgacgcccgc
     2701 cccctcggcg gcgtcgtccg cggccccgcc cttgaggccg atcaactcgc acttgtaggg
     2761 ctcgtcggcc agctcggcga gcgcgtcggc gtcggagatc tcgcggcggt ggaaggtctg
     2821 gccctcgttg atgatccgct gcatgacctt ctcgagggcc ttgagatcct cgggattgaa
     2881 cggcgtctcg acgtcgaagt cgtaatagaa gccgtcgcgg atcggtgggc cgatgccgag
     2941 cttggcctgc gggttgacct cctgcaccgc ctgggcgagc acgtgggccg tggagtggcg
     3001 cagcacgtcg aggccgtcct gctcggccgc cgtgaccggt tcgacggtgt cgccgtcggc
     3061 gaggacgtgc gcgaggtcgc gcagctcgcc gttgacgcgg gcgaccagga cggcccggtc
     3121 gtcgccgaac aggtcggcgg cggtggtctg ctccggcacc gatcgctcgc ttccggcgat
     3181 attgacggtg atctgggcag acacgttttc tctcctggta tgcggtggcg ccgggctcgg
     3241 gtgccgagac ggccggttct cgccgcgcgg tgcgcgaccc gccaactcta ccgagcggta
     3301 tgccgccccc tcacccgcgt ttacgccccg cacccccgtc gagatgtcga ctcgacccca
     3361 tcgagatgcc ggccagggtg gtcagggggg ccgggcgcag cggaagccga ggtgctgcag
     3421 ccggccgcaa tcgggtgtca ggacggctca tgcctcacgg agggtctcct cgagcaaggc
     3481 cttgaggtcg tccgggaggt cctgttcgcg gatcgacacc tcggtcgggg gtgtcgggca
     3541 gctgacgcca tagcaatagg tgtccgggtg ctgccgggcc gcgctcgccg cggcctgcgc
     3601 gtcgagagtc tgcagggtgc gattgcccag gacgctgcgc cactgctgct gttgcggggc
     3661 cggcagctcg tcgagagcga ccgagcggcg cgcggtcaac ccggcgacac cgccactgcg
     3721 cgtcacctct acgaccgccc ccgcgggcac ctctccttcg tgctccatgt cgtccgggcc
     3781 gcgctcgtga tggccgactc ccccctcctc cggcacgccc ggccactccg ggctcggctc
     3841 gccggaatcg tcgggcacgg gcgcgggaac cggctcgccc ggatccgcgg gcaggtcgac
     3901 gaccggcacc ggcccctcac cgtcggactc ggcgccgccg agcacaccca cctgcgccca
     3961 cgcggacttg acgg
//
LOCUS       DAOPJG010000251         3908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_25624_length_3908_cov_8.448222, whole genome shotgun sequence.
ACCESSION   DAOPJG010000251 DAOPJG010000000
VERSION     DAOPJG010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25624_length_3908_cov_8.448222"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKU75_15735"
     CDS             complement(<1..233)
                     /locus_tag="PKU75_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HPF82377.1"
                     /translation="MMRSLGSRAPGGNEGVETGAAIADFAMVTGLLMFVFAAVFQLGL
                     TLHIRNTLISCAAEGARVGARVDAAPGDAVARTT"
     gene            complement(233..448)
                     /locus_tag="PKU75_15740"
     CDS             complement(233..448)
                     /locus_tag="PKU75_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82378.1"
                     /translation="MSEKMIKAHIRLVDGLRRALADREGQRGDVPGWVLITIMTAGLV
                     SAIWFIADERLKSLIGDALDEVSPSGN"
     gene            complement(487..1437)
                     /locus_tag="PKU75_15745"
     CDS             complement(487..1437)
                     /locus_tag="PKU75_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPF82379.1"
                     /translation="MNVVERVGIGQWGALTGLFFASGVLLILRHLRAQRRPTLEARLV
                     PYLIDTVGFTRLRDRTVISPAGVLAVLGRPIVRDGARLVERLLGGADSVRSRLVRAGR
                     QPDVDRFRAEQVLAGVAGGLAGGLLGVLLAVGGNGGPLMLLWLTGLGALAGVVAWDQW
                     LTRAVMRREARMLAEFPTVAELLALAVGAGEGAVGALERVCRLSKGELATELGRCLAD
                     ARSGASLPRALGGLAERTGLTSLSRFVDGVVIAVERGTPLADVLRAQAQDVREEGRRQ
                     LMETGGKKEIAMMIPVVFLVLPVTVLFAVFPGFDFLNFSM"
     gene            complement(1434..2306)
                     /locus_tag="PKU75_15750"
     CDS             complement(1434..2306)
                     /locus_tag="PKU75_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPF82380.1"
                     /translation="MPPVLVGLILGLGLFCIWWSFWPTEESAATTTGRGRSRIDRLGD
                     DLVQAGYAAVGPRALLATCALTGVLVFVLFYGMVSVASVALSFGLLASYAPIALVRGR
                     ARRRRAALRDLWPDAVDNITSGVRAGLALPESLVQLAIRGPLPLRPAFQQFAEDYRVS
                     GRFNDSLDRLKANLADPVGDRLVEALRMAREVGGSDLGRLLRTLSGFLRDDAHARSEL
                     ETRQSWTVNAARLAVAAPWIVLALMCTRPEAVQSFSTATGALVLAGGGTCTLIAYRIM
                     VRISRLPEEERVLG"
     gene            complement(2308..3537)
                     /locus_tag="PKU75_15755"
     CDS             complement(2308..3537)
                     /locus_tag="PKU75_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPF82381.1"
                     /translation="MDAAVALIESDVREHIRRSDLDPLADAHEIRRVVRDAVADYDDR
                     SISGGLPRLSDLDAAARAILDSVAGYGPLQQYFDDPAVEEIWINEPWRVFIARNGVSE
                     LTPTMLTDEAVRDLVERMLRTSGRRVDLSSPFVDAVLPDGSRLHVVIPDITRAHWFLN
                     IRKFVAKADKLEDLVRLGTLTPEASRFLEAAVVSGLNVLVAGGTQAGKTTLLNCLAAA
                     VPPRERIVTVEEVFELKIPQRDVAAMQCRQPSLEGSGEVPLRRLVKEALRMRPSRIIV
                     GEVRQAESLDLLIALNSGLPGMATVHANSAREAVIKMCTLPLLAGENVGSRFVVPTVA
                     SSIDIVVHTGLDRDGKRRVREIAAIPGRCEGDVVEMADLFVRRGGELRRADGFPPHAD
                     RFEASGFDLAQLLRSGV"
BASE COUNT          677 a         1339 c         1336 g          556 t
ORIGIN      
        1 gtcgtgcgcg ccacggcatc tcccggtgcc gcatcgacac gtgccccgac gcgtgcgccc
       61 tcggcggcgc acgagatcag ggtgttgcga atgtgcaggg tcagcccgag ctggaagacg
      121 gcggcgaaca cgaacatcag caggccggtc accatggcga agtcggcgat cgccgcgccc
      181 gtttcgactc cctcgttccc gcccggtgcc cgcgagccca ggctgcgcat catcagtttc
      241 cggacgggct gacctcgtca agggcgtcgc cgatcaggga cttgagccgt tcgtcggcga
      301 tgaaccagat cgccgagacc aggcccgccg tcatgatggt gatcaggacc cagccgggca
      361 catcgccgcg ctggccctcg cggtcggcga gggctcggcg caggccgtcg acgaggcgga
      421 tgtgagcttt gatcatcttc tcggacatag cgtttcctct cggttgtgct cgattgagcg
      481 ttggtgtcac atggagaagt tcaggaagtc gaagccgggg aatacggcga acaggacggt
      541 gacgggcagc acgaggaaga cgaccgggat catcatggcg atctctttct tgccgccggt
      601 ctccatcagt tggcggcggc cctcctcacg aacgtcctgg gcttgcgccc gcaggacatc
      661 cgccagcggg gtgccgcgtt cgacggcgat cacgacgccg tcgacgaaac ggctgaggga
      721 cgtcaggccg gtgcgttcgg cgagaccacc cagcgcccgc gggaggctgg cgccggatcg
      781 ggcgtccgca aggcagcggc cgagttcggt ggcgagttcg cccttggaga ggcggcatac
      841 cctttcgagt gccccgacgg caccctcgcc tgcaccgaca gcgagcgcga gaagctctgc
      901 cacagttggg aattcggcga gcatgcgcgc ctcgcgcctc atgaccgcac gcgtgagcca
      961 ttggtcccac gccaccacac cggccagcgc gcccaggccg gtgagccaca gcagcatgag
     1021 cggacccccg ttgccgccga cggcgagcag aacgcccagc aggccgcctg ccaacccgcc
     1081 cgcgacgccg gccaggacct gttcggcgcg gaagcgatcc acatccggct ggcggcccgc
     1141 gcggaccagg cgggagcgca cggaatcggc gccgccgaga aggcgttcga cgagcctggc
     1201 tccgtctcgc acgatcggtc ggcccagcac cgccaagacc ccggcggggg agatgaccgt
     1261 tcgatcgcgt aggcgggtga acccgaccgt gtcgatgagg taggggacca ggcgggcctc
     1321 caaggtgggc cgacgctgcg cgcgcaggtg ccgaaggatc agcagcaccc cggatgcgaa
     1381 gaacaggccg gtcagcgctc cccactggcc gatgccgacg cgttcgacga cgttcatccg
     1441 agcacccgct cttcctcggg cagccgcgag atccgaacca tgatccggta ggcgatcaag
     1501 gtgcacgttc cgccgcccgc gagcaccagg gcaccggtcg cggtcgagaa cgactgcacg
     1561 gcctccgggc gagtacacat gagtgccagc acgatccacg gggcagcgac cgccaggcgg
     1621 gcggcattga ccgtccacga ttggcgggtc tccaactcgc tgcgcgcgtg cgcgtcgtcg
     1681 cgcaggaagc ccgatagcgt gcgcagaagc cgcccgagat ccgagccgcc gacctcgcgc
     1741 gccatgcgca gcgcctcaac cagccggtcg ccgaccgggt cggcgagatt ggctttcaga
     1801 cggtccaggc tgtcgttgaa gcggccgctg acccgatagt cctcggcgaa ctgctggaat
     1861 gccggacgta gggggagcgg gccgcgaatc gccaactgca ccaacgactc cggcaacgcc
     1921 agacccgccc gcacaccgga ggtgatgttg tccacggcat ccggccacag gtcgcgcaga
     1981 gccgcccggc gccggcgagc ccgcccacgc acgagggcga tgggtgcata cgacgccagc
     2041 agcccgaagg acaaggccac cgatgccacc gacaccatcc cgtagaagag gacgaagacg
     2101 agcaccccgg tcagcgcgca ggtggcgagg agggcgcggg gcccgaccgc ggcatacccg
     2161 gcctgcacca agtcgtcccc gaggcgatcg atccggctgc gaccgcgccc tgtggtcgtg
     2221 gccgccgact cctccgtcgg ccaaaaggac caccagatgc aaaaaaggcc aagacccagg
     2281 atcagtccca cgagtaccgg tggcatgtca gacccccgac cgcagcaact gcgcgagatc
     2341 gaacccgctc gcctcgaaac ggtcggcgtg aggcgggaag ccatcggcac gccgcagttc
     2401 gcctccgcgg cgcacgaaga gatcggccat ctcgacgaca tcgccctcgc agcgccccgg
     2461 gatggcggcg atctcgcgca cccggcgctt gccgtcacgg tccaatccgg tgtgcaccac
     2521 gatgtcgatc gatgacgcga cggtcgggac gacgaaccgg ctgccgacgt tctcacccgc
     2581 cagcaacggg agggtgcaca tcttgatgac ggcctcgcgg gcactgttgg cgtgcaccgt
     2641 cgccatgccg ggaaggccgg agttcaacgc gatcaggagg tcgagggact cggcctggcg
     2701 cacctcgccg acgatgatgc gcgatgggcg catccgaagg gcctccttga ccagccgccg
     2761 caacggcacc tcgccgctac cctccagact gggctggcgg cactgcatcg cagcgacgtc
     2821 gcgttggggg atcttcagtt cgaagacctc ttccaccgtg acgatccgct cccgcggtgg
     2881 cacggcggcg gccaggcagt tgagcagggt cgtcttgccc gcctgagtgc cgcccgcgac
     2941 caagacattg aggccggaaa cgacagcggc ttcgaggaaa cgactggcct ctggtgtcaa
     3001 agtgccgagg cggacaagat cttccagctt gtcggctttt gcgacgaatt ttctgatgtt
     3061 caggaaccaa tgagcccgcg taatgtcggg aatgacgacg tgtaggcggc tgccgtccgg
     3121 caggacggcg tcgacgaacg gcgagctgag gtcgacccgg cggccggagg tgcgcagcat
     3181 gcgttcgacc aggtcgcgca ccgcctcgtc ggtgagcatc gtcggggtca gttcggacac
     3241 accgttgcgg gcgatgaaga cgcgccaggg ctcgttgatc cagatctctt cgacggctgg
     3301 gtcgtcgaaa tactgttgca gcggaccgta tcccgcgacc gagtcgagga tcgcgcgggc
     3361 ggcggcgtcg aggtcggaca ggcgcggcag gccgccgctg atcgaccggt cgtcgtagtc
     3421 ggcgaccgcg tcccgcacga cccggcggat ctcgtgggcg tcggccaatg ggtccagatc
     3481 ggaacgccga atgtgctcgc gcacatcgct ctcgatcagc gccaccgcag cgtccatctc
     3541 tttcccccct tccccgagag gtcttcccga ggccccctgc gcctcgggtt tgaccgggac
     3601 cataggcgag gtcggcgaat cccgctcggt gagtgagggg ttatccacag aagctggaga
     3661 aactttgcca agttcttgcg cggtctttac ctttccacgc cctcgacgac ctcgccgacg
     3721 acccgcatag cgcgtgtcac aacctgggcg atccggcgtg cccgattgcc cgactcgaga
     3781 cgaggccggg cggttcgtgt cacgacttgg gcgatggccg gcgacggatc ccccacgtgg
     3841 tgacatccgc cggtgtgggc tcgacgtgcg gtggccggcg gcgagccgac aatcgggtgg
     3901 ttcgacat
//
LOCUS       DAOPJG010000252         3881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_25870_length_3881_cov_9.255619, whole genome shotgun sequence.
ACCESSION   DAOPJG010000252 DAOPJG010000000
VERSION     DAOPJG010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25870_length_3881_cov_9.255619"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..3201)
                     /locus_tag="PKU75_15760"
     CDS             complement(334..3201)
                     /locus_tag="PKU75_15760"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPF82382.1"
                     /translation="MSATLTERPTAPASPPPPVARGGSRMQRWLNSWRVSLKMARRDA
                     LRYKGRSALVILMVALPVALIVGGLTLSATSFTSAAEALPRTLGSAVARVDTIQTTVV
                     QQGVDGSNSYQVSGPSPEDQPPKAKPIPGVDPETGRPSEIAAALGVFLGGTVKAVQSG
                     DFRWHDGDRRPWGDATFFDMKQDFGERAKLTDGRWPTDNSEVVVTPRAVHEGLPTSGT
                     ITLTVGGEDRVVTIVGQADVASAWGGMTMLATTKPWVSEGPSGPASASYLLFRDAPFT
                     WSEVRNANEHGLSITSREVILNPPAASELPADIYQQDVENAKSLRIAAAMLGATLFLL
                     TALLVGPAFAVSAGRQRRSLALAASNGAETRQLRRSVLASAVVLGAIAVLIGAALGAA
                     VALAAAAIWTRMRPWDQMFGPAEIPWLGVLIVVVCAVLAALFAALIPALKLGRLDIVG
                     VMKGQNVSPPINRKLPIVGAVLFALGVVGVFGAVVQESAVMAALGVFGLFLGPICLIP
                     LLLVACGKLASRFSAPVRMASRDAARQRHRSAPTVAAVMAGAALLSTFAVALASDTKF
                     QAKLYTPRNIAGEASTWVDPATMPALLASLQANAPTWRTAEQRYIAADYGTPGAEKKP
                     TEEPFLSVQPPGCTVADTMPNYEGAPSPETEKCAVAGTNGGMSMRAGVMVTPAAEIIR
                     RVGATGAQAQAIRDGAVLVGDPAWTSGGKVTLAYGGQPIPTDANPTGPAQPTWSKSTA
                     YPAIVIDEATYRRGAVESYTGVVLTQELVEKLKLPTTMSGLYIYDPNGPISKDAEDKV
                     NDKLGEDMRIQVERGFQRSDWKIMLIVFGIAGFLLTTVTLISTALALAEQQADMGTLA
                     AVGATKGTRRRFAGAQAATVALVGVALGTLIGLVAGVAIAYPSTSRGWDEALQKEVTL
                     SPTIGIPIWHLLSILIGVPLVAALVAAVSIRRAPHVTRRGN"
     gene            complement(3207..>3881)
                     /locus_tag="PKU75_15765"
     CDS             complement(3207..>3881)
                     /locus_tag="PKU75_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF82383.1"
                     /translation="GETAVHALRGVSLDVFPGELVAVMGPSGSGKSTLLNLAGGLDKA
                     TSGQVLIEGDEVGALGANQLASLRRRRVGFVFQDFNLIPSLTAAENVSLPLELDGMSV
                     KEGRRAAHEILEIVGVAELADRYPDKMSGGQQQRVAIARALVGDRRLVLADEPTGALD
                     STTGEGVLRVLRDRCNAGAAGLLVTHEARHAAWADRVVFLRDGVVVDTTGSPEEAETL
                     LVGSGS"
BASE COUNT          575 a         1278 c         1465 g          563 t
ORIGIN      
        1 ggcccacacg cgtcaccacg ccgaacccgc ctgcccggtt aagttcgaag agcccgcgaa
       61 cccctatgag aacagcaccg tgatgatgac caacgtgatg gccgtcttcg ggcccaaact
      121 gccgctcgag ctgtccgcga actgcctgag ggagcactgg ggcgggctga cggagggcaa
      181 ccacttcccc gacttcgagg gcgaggccaa gacctgcacc gagacgctcg cgaaggggcg
      241 gccctatttc gcggcggggg gcgacgagct aggagcgcgg ccggaccctc ccctgcgagg
      301 cccggccgcg ctcgtcgctg tgacccgtcg gggtcagttg ccccggcggg tgacgtgcgg
      361 tgcccggcgg atgctgacgg cggcgacgag cgctgcgacg agcgggacgc cgatcaggat
      421 ggacagcagg tgccagatcg gtatgccgat cgtcgggctc agcgtgacct ctttctggag
      481 cgcttcgtcc cagccacgcg aggtgctcgg gtaggcgatg gcgacgcccg cgacgaggcc
      541 gatcagtgtg ccgagggcga ctccgaccag agcaacggtg gccgcctgcg ctccggcgaa
      601 ccggcgccgg gtgcccttgg tcgcgccgac cgcggcgagg gtgcccatgt cggcctgctg
      661 ctccgccaac gcgagcgccg tgctgatcaa ggtgacagtg gtcagcagaa agccggcgat
      721 gccgaagacg atgagcatga tcttccagtc gctgcgctgg aagccgcgct cgacctggat
      781 gcgcatgtcc tcgccgagct tgtcgttgac cttgtcctcg gcgtccttgg agatggggcc
      841 gttggggtcg tagatgtaga gcccgctcat cgtggtcggg agcttcagct tctccaccaa
      901 ctcctgggtc aggaccacgc cggtgtagct ctcgacggct ccgcgccgat acgtcgcttc
      961 gtcgatgacg atcgcggggt atgccgtgct cttggaccac gtgggttggg ccggcccggt
     1021 ggggttggca tcggtgggga tgggctgccc gccgtaggcc agcgtcacct tgccgcccga
     1081 ggtccaggcc ggatcgccca cgaggacggc accatcgcgg atggcctggg cctgcgcgcc
     1141 ggtggcgccc acccggcgga tgatctccgc agcgggcgtg accatgacgc cggcgcgcat
     1201 ggacatgccg ccgttggtgc cggccaccgc acacttctcc gtctctggag agggtgcgcc
     1261 ttcgtagttc ggcatcgtgt cggccacggt gcagccggga ggctggacgc tgaggaaggg
     1321 ctcctcagtg ggcttcttct ccgctcccgg cgtgccgtag tcggcggcga tgtagcgctg
     1381 ctcggccgtg cgccacgtcg gggcgttggc ctgtagtgac gccagcagag ccggcatggt
     1441 cgcggggtcc acccacgtcg aggcctcgcc ggcgatattg cgcggggtgt agagcttggc
     1501 ctggaacttc gtatcgctcg cgagggcgac ggcgaaggtc gacaacaagg cggcgccggc
     1561 catcaccgct gcaacggtgg gtgcggagcg gtggcgctgc cgggcggcat cgcgcgacgc
     1621 catgcggacc ggcgcggaga agcgcgaggc gagcttgccg caggccacga gcaggagcgg
     1681 gatcaggcag atcgggccca agaacagacc gaagaccccc agagctgcca tcaccgcgga
     1741 ctcctgcacg acggcgccga agacaccgac cacgccgagc gcgaagagga ccgcgccgac
     1801 gatcggcagc ttgcggttga tcggcggcga gacgttctgc cccttcatca ccccgacgat
     1861 gtcgaggcga ccgagtttga gtgctgggat cagtgcggcg aagagggcgg ccaggacggc
     1921 gcagacgacg acgatgagca cgcccagcca cgggatctcg gcgggaccga acatctgatc
     1981 ccagggccgc atccgggtcc agatcgcggc ggcggcgagg gcaacagcgg cgcccaaggc
     2041 cgccccgatg agcacggcga tcgcgccgag gaccacggcc gaggcgagga cgctgcggcg
     2101 cagttgccgg gtctcggcgc cattgctcgc ggcgagggcg aggctgcgac gctgccggcc
     2161 cgcgctgacc gcgaaggcgg gcccgacgag cagagcggtc aggaggaaga gcgtggcgcc
     2221 gagcatcgcg gccgcgatcc gcaggctctt ggcgttctcg acgtcctgct ggtagatgtc
     2281 cgcgggcaac tcggacgccg ccggcgggtt gaggatgacc tcgcggctgg tgatgctcag
     2341 gccgtgttca ttggcgttgc gcacctcgct ccaggtgaac ggagcgtcgc ggaagaggag
     2401 ataggacgca ctcgccggcc ctgacggccc ttcgctcacc cacggtttcg tggtggcgag
     2461 catggtcatg ccgccccagg cggacgcgac gtccgcctgc ccgacgatcg tgacgacccg
     2521 gtcttcgccg ccgacggtga gggtgatcgt cccgctcgtc ggcagcccct catggaccgc
     2581 gcggggggtg acgacgacct cggaattgtc ggtcggccaa cgcccgtccg tcagcttggc
     2641 gcgctcgccg aagtcctgct tcatgtcgaa gaaggtggca tcgccccagg gccgccggtc
     2701 cccgtcgtgc cagcggaagt cgccgctctg gacggccttg acggtgccgc cgagaaagac
     2761 gccgagggcc gcggcgatct cgctcggccg gcccgtctcg ggatcgacgc ccgggatggg
     2821 tttggccttc ggaggctggt cctcggggct cgggccgctc acctgatagg agttggatcc
     2881 gtcaacgccc tgctgcacca ccgtggtctg gatggtgtcg acgcgggcga cggccgagcc
     2941 gagcgtgcgc ggcagggcct cggcggcgga cgtgaagctg gtggccgaga gcgtcaggcc
     3001 gcccacgatg agtgcgacgg ggagggcgac catgaggatg accagcgcgc ttctgccctt
     3061 gtagcgcaac gcatcccggc gcgccatctt gagggagacc cgccaggagt tgagccagcg
     3121 ctgcattcgg ctgccgccgc gggcgacggg gggcggtggg ctcgcgggcg ccgtggggcg
     3181 ctcggtgaga gtggccgaca tcgtggtcag ctccccgatc cgacgaggag agtctcggcc
     3241 tcttcgggcg aaccggtcgt gtcgacgacg acgccgtcgc gcaggaacac cacccggtcg
     3301 gcccaggcgg cgtgccgggc ttcgtgggtg acgagcagcc cggcggcgcc ggcgttgcag
     3361 cgatcacgca gcaccctcag gacgccttcg ccggtcgtgg aatcgagggc gccggtgggc
     3421 tcgtcggcga ggaccaggcg gcggtcgccg acgagggcgc gcgcgatcgc gacccgttgc
     3481 tgctggccgc cggacatctt gtcgggatag cggtcggcga gttcggcgac gccgacgatc
     3541 tccaggatct cgtgcgctgc gcgacgaccc tccttgaccg acatgccgtc gagttccagt
     3601 gggagagaga cattttcggc ggcggtgaga gaggggatga ggttgaagtc ctggaagacg
     3661 aagccgaccc ggcgccggcg cagcgaggcg agctggttcg cgccgagcgc gcccacctcg
     3721 tcgccctcga tgagcacctg gccgctggtg gccttgtcga ggccgccggc gaggttgagc
     3781 agggtggact tgccggagcc gctgggcccc atgacggcga cgagttcgcc cgggaagacg
     3841 tccaggctga cgccgcgcag ggcgtggacg gcggtctcgc c
//
LOCUS       DAOPJG010000253         3840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_26258_length_3840_cov_5.336328, whole genome shotgun sequence.
ACCESSION   DAOPJG010000253 DAOPJG010000000
VERSION     DAOPJG010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26258_length_3840_cov_5.336328"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKU75_15770"
     CDS             complement(<1..228)
                     /locus_tag="PKU75_15770"
                     /inference="COORDINATES: protein motif:HMM:NF023275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HPF82384.1"
                     /translation="MTTTAAKDEVIVWRTRLIAVCVGLVALTFRQLPGRIVPDTKLDL
                     TVSPWSFLGDALHLWDPQGYFGQLQNQAYGYL"
     gene            complement(225..992)
                     /locus_tag="PKU75_15775"
     CDS             complement(225..992)
                     /locus_tag="PKU75_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF82385.1"
                     /translation="MQPPDPAVRARSAANIGRSIRLFRAFLVEQSDPDRFYSALAEDS
                     VSQAGAWCRLSGARVLDVGGGPGYFAAAFERAGSRYVGVEPDAGELTARGPATGTAVR
                     ASGLALPFPSGAFDLTFSSNVLEHVSTPEEMAQEMVRVTAPGGTVVLSWTPWLSPWGG
                     HETAPWHYLLGGRGAAERYARRHGHRPKNDYGTSLFACSVARMARWLEEARAHGQVRV
                     VRVLPRYHPWWAQWVIRVPGLREVVSWNAMIVLTKVA"
     gene            1100..2365
                     /locus_tag="PKU75_15780"
     CDS             1100..2365
                     /locus_tag="PKU75_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82386.1"
                     /translation="MTQFARSRASLQRRRGALARKAEPETPRNPLARSGAVLAVATVV
                     AGVLGYVYILVLTRALGPVGYGVLGSLLGLSVVLSIASTALQLEATRAVAIDPSAPRG
                     WLHRRGIAISFATGALTLLATPLIDAVLRLPTPTLVFALAAVMLPQTYVGVQLGILLG
                     AGRTGAFSLLLVVSGASRTAAAVLTAVLHQSPAFALWVSAATGVATNVIGALLIRRLP
                     RPHLASDHPPIGWSGFLRAALGAGALIVLLNADLLTARAVLPDKESGWYAFLTVFGRV
                     TFWGTNFVALWVFPHEAARESAAKARAYALTAVAVIATFAVASSWLAGDVITDTLAGP
                     EYAGAAGYAPWFAVAGSLLSVVQLATYVDVARGRRRLSLVVWAASPAMVLAIWLAPHT
                     IAGVISAAVVILTVVAAAGVASMRRSAAR"
     gene            complement(2395..3294)
                     /locus_tag="PKU75_15785"
     CDS             complement(2395..3294)
                     /locus_tag="PKU75_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82387.1"
                     /translation="MRDGRRLNEIDAYCWGANDRGQLGLGTVSEREESPRRLSAMPGA
                     VRSIAAGGAHACLTVRGRQDALFCWGDNRFGQLGIGSVSAYEASPAHVADFFGGGSPL
                     GDDSTCMDDLFGHAYCWGRNDRGQLGDGTRTNRSVPTRVKGLDTVSDVSSGGSHACAL
                     TTSRRVKCWGDNTMGQLGNGGSGVSTRPVAVRGLPRNPSWLSMGQRHSCAVDYDTQKM
                     FCWGDNRSGQLGDGTLTVRRTPVPVQRLGRTYYEAAMGNRFTCVRNPDTTLKCWGANE
                     IGQLGNGTTIPRRWPTSVADPVS"
     gene            complement(3273..3578)
                     /locus_tag="PKU75_15790"
     CDS             complement(3273..3578)
                     /locus_tag="PKU75_15790"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82388.1"
                     /translation="MVGKRKLGGLAVAVAAFAGVGISSAGAADAGPDFWQVAAGARHA
                     CAITDTGELYCWGDDGRGQLGNGSDLGSDTRPRRVDLPGRVWMAALGADFACAMAGG"
BASE COUNT          533 a         1404 c         1292 g          611 t
ORIGIN      
        1 caggtagccg tatgcctggt tctgcaactg tccgaagtag ccctggggat cccacaggtg
       61 cagcgcatcc cccaggaagg accacggcga cacggtcaag tcgagtttgg tgtccgggac
      121 gatccggccg ggcagctggc ggaaggtgag cgcgacgagg ccgacgcaga ccgcgatcag
      181 ccgggtccgc cagacgatga cctcgtcctt ggcagcggtt gtcgtcatgc gaccttggtg
      241 agcacgatca tggcgttcca actcaccact tcgcgcaggc ctggaacgcg gatgacccac
      301 tgcgcccacc atgggtgata gcggggcagc acccggacca cgcggacctg cccgtgcgcc
      361 cgtgcctcct cgagccagcg cgccatgcga gcgaccgagc atgcgaacag actggtgccg
      421 tagtcgttct tcggccggtg accgtgccgg cgggcgtagc gctcggctgc accgcgaccc
      481 ccgaggaggt agtgccaggg tgcggtctca tggccacccc acggggagag ccacggtgtc
      541 cacgagagca cgacggtccc gccgggggca gtgacgcgca ccatctcctg cgccatctcc
      601 tccggggtgg acacgtgctc gagcacattg ctggagaagg tcaggtcgaa cgcccccgac
      661 gggaacggaa gggcgagccc ggaggcgcgc accgctgtcc cggtcgccgg tccacgcgcc
      721 gtgagttcgc cggcgtcggg ctcgacgccg acataccgag acccggcgcg ctcgaaggcg
      781 gccgcgaagt agccaggccc gccgcccacg tcgagcactc gggcgccgct cagccggcac
      841 cacgcgccgg cctgcgacac cgagtcctcg gcgagggcgg agtagaagcg gtcgggatcg
      901 gactgctcga cgaggaaggc cctaaagagc ctgatggagc ggccgatgtt ggctgccgac
      961 cgagcgcgaa cggcggggtc gggtggctgc acgcgggcat tgtagtcagg cctcttcgga
     1021 attgttgcgc cggcgcgacc agcgcccgcc gcggggtgac gtaacctttc ccgagcaagg
     1081 gcaaggcgat aggagcggcg tgacccagtt cgcacgctcg cgcgcctcgc tgcagcgtcg
     1141 tcgaggcgcg ttggcccgga aggccgagcc ggagacccca cgcaatcccc tggcccggtc
     1201 gggcgctgtg ctcgcggtcg cgacagtggt cgccggcgtg ctcggctacg tctacatcct
     1261 cgtcctgacc cgggctctcg gccccgtggg ctatggcgtg ctcgggtcct tgctcggact
     1321 ttcggtggtg ctctcgatcg cctcgacggc cctgcaactc gaggccacgc gggcggtcgc
     1381 gatcgacccg tccgcgccgc ggggatggct ccatcgccgc ggcatcgcca tctcgttcgc
     1441 caccggcgcg ctgacgctcc tcgccactcc gctcattgat gccgtcctac gattacccac
     1501 tcccacactg gttttcgcgc tggccgcggt gatgctgccg cagacctatg tcggcgtgca
     1561 gctcggcata ctgctcggcg cgggccggac gggagcattc agcctgttgc tcgtcgtcag
     1621 cggcgcgagc cgcaccgccg cggccgtgct gaccgcggtc ctccaccagt cccccgcgtt
     1681 cgccctgtgg gtgtccgccg ccaccggcgt ggccacgaac gtcatcgggg cactcctgat
     1741 ccgtcgccta ccccgcccgc acttggcgtc cgaccatccg ccgatcgggt ggtccggatt
     1801 cctgcgggca gccctcgggg ccggcgccct catcgtcctc ctcaacgccg acctgctgac
     1861 ggcccgagca gtcctgcccg acaaggagtc cggttggtac gccttcctga ccgtcttcgg
     1921 gcgggtcacc ttctggggga ccaacttcgt cgccttgtgg gtcttcccgc acgaggcggc
     1981 gcgcgagtcc gcggccaagg cccgggccta tgcgctgact gcggtagcgg tgatcgcgac
     2041 cttcgcggtc gcctcatcat ggctcgccgg cgacgtcatc accgacaccc tcgccgggcc
     2101 ggagtatgcc ggtgccgccg gctatgcgcc ctggttcgcc gtcgccggca gcctgctgtc
     2161 ggtggtgcag ctggcgacct acgtcgacgt cgcccggggc cggcgccggc tctcgctcgt
     2221 ggtgtgggca gcgtcgccgg cgatggtgct cgcgatctgg ttggcaccgc atacgatcgc
     2281 cggcgtcatc tccgccgccg tcgtcatcct caccgtggtc gccgcggcgg gggtggccag
     2341 tatgcggcgc tcggccgcgc gctgagccgc ccgcggccga gcatcgacag ccgctcagct
     2401 caccgggtcg gcgacgcttg tgggccaccg gcgcggaata gtggtgccat tgccgagctg
     2461 cccgatctcg ttggcgcccc agcacttcag cgttgtgtcg ggattgcgca cacacgtgaa
     2521 gcggttgccc atggcggcct cgtagtacgt cctgccgagg cgctgcactg gcaccggggt
     2581 gcggcgtacc gtcagagtcc cgtcaccgag ctgacccgag cgattgtcgc cccagcagaa
     2641 catcttctgg gtgtcgtagt ccacggcgca gctgtgtcgc tgtcccatgc tgagccacga
     2701 gggattgcgc gggagccccc gtacggcaac cggccgggtg ctcacgcccg acccgccgtt
     2761 gcctagctgc cccatcgtgt tatcccccca gcacttgacc cgacggctcg tcgtcagggc
     2821 gcaggcgtgc gaccccccgc tggagacgtc ggacaccgtg tcgagacctt tcacgcgcgt
     2881 cgggacggac cggttggtgc gcgtcccgtc gccgagctga ccgcgatcgt tgcggcccca
     2941 gcagtacgca tgtccgaaca ggtcgtccat gcacgtgctg tcgtccccga gcggagaacc
     3001 accgccgaag aagtccgcga cgtgcgcggg gcttgcctcg taggcagata ccgacccgat
     3061 gcccagttgg ccgaaccggt tgtcacccca gcagaacagc gcgtcctgtc gaccgcgcac
     3121 cgtcaagcag gcgtgcgccc cgccggcggc gatgctccgc accgcacccg gcattgcgga
     3181 caaccggcgc gggctttcct cgcgctcgga gacggtcccc agccccagct gcccgcggtc
     3241 attggcaccc cagcagtagg cgtcgatctc attcagcctc cggccatcgc gcacgcgaag
     3301 tccgccccca aggcggccat ccacacccgt cccgggaggt cgacgcgtcg cggccgcgta
     3361 tcgctcccga ggtcggagcc gttgccgagt tggccgcggc cgtcatcacc ccagcagtac
     3421 agctcgccgg tgtcggtgat ggcgcaggcg tgccgggccc cagccgccac ttgccagaag
     3481 tctggtcccg cgtcggcggc acccgcgctg ctgatcccca cacctgcgaa cgcggccacc
     3541 gccaccgcaa gtccgccgag cttgcgcttg cccaccatgt cattccccct tcgtgtcccc
     3601 cgaaaacccc tacggtcgta gcggctggtc tcgacgctat caaggccgat tcgtccttgt
     3661 gcgagaatcg gaaaccgatg cgaaagctgg tccttcaccc aaggaggcgc ggctcaatgc
     3721 gttcttcggt gacgttgccg tcggcgtcgc gctggacgac atacgcgccc ttgccgggct
     3781 gctcggtgac cgcctcgacc aggtcggtgc cgtggtaaac gatgccgacg ccgtcgtcgg
//
LOCUS       DAOPJG010000254         3836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_26295_length_3836_cov_7.350172, whole genome shotgun sequence.
ACCESSION   DAOPJG010000254 DAOPJG010000000
VERSION     DAOPJG010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26295_length_3836_cov_7.350172"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..903)
                     /locus_tag="PKU75_15795"
     CDS             complement(55..903)
                     /locus_tag="PKU75_15795"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF82389.1"
                     /translation="MTGLYGVIDRIASMAGAALGGRLIAWVGAPGALYADAASFLVCA
                     LILGLTAGSPPLVPVADRDPVPYVQKLRRGWDFLRRDVVLVGMSAIVAATNLLDLAWS
                     AVLMPAWGRNSGHGAAGIGVLFAVFSACAALSSMAAARWAERLPRFRTYVIAYLLTGL
                     PRFAVLALGSPLCVCVAVFVIGGLACGFLNPILGAVVFERIPERLVGRVSALNSALCW
                     SLMPLGGLLGGLLAASLGLAPVLWVVGTAYLLVTLLPLLVPSFREFDSRPAPEPVRAR
                     TSGHSV"
     gene            complement(909..1277)
                     /locus_tag="PKU75_15800"
     CDS             complement(909..1277)
                     /locus_tag="PKU75_15800"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF82390.1"
                     /translation="MANAISLTGTRISMIALPWFVLTTTGSAAHTGLVAAVEMTPLVL
                     FEVLGGPFADRYGGRAVALSCDPASAVVVAAIPLLHGIGGLSFPVLLVLVGVAGALRG
                     PGDGAKAALIPRSRRPRECR"
     gene            complement(1304..1909)
                     /locus_tag="PKU75_15805"
     CDS             complement(1304..1909)
                     /locus_tag="PKU75_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPF82391.1"
                     /translation="MPRQYVIAVMASNSLNAKSTFQTSALAHPARLRMLGLLRLEGPA
                     TATSLARRLGLNTGATSYHLRQLAQHGFIEEDAGRGNGRERWWRAAHQSTVTDSSALS
                     TPEEHETHGAYVQAVGILYAEQLQRAVERHDLLPSPWRDAGTFSDWQLRLTPERAQGL
                     RDRLVAVIEEYAEDADAAAATFVVNLNAYLHPDAVPERGQW"
     gene            complement(2024..2503)
                     /locus_tag="PKU75_15810"
     CDS             complement(2024..2503)
                     /locus_tag="PKU75_15810"
                     /inference="COORDINATES: protein motif:HMM:NF022240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="HPF82392.1"
                     /translation="MRWATRCCRVTAGMLAGAGLLAGCGEEHPTQASGSSVNSPSSAG
                     STATSPRTAPRLTSSSPPGQPDLIAGVRLVEAEGGASYEVTPREALRDHLSAAALDAA
                     WGEVTAAYPEADTDGLHDQFVCHAYFANAKPTWDLEPWRADLTYPETVATACNPPRA"
     gene            2540..3733
                     /locus_tag="PKU75_15815"
     CDS             2540..3733
                     /locus_tag="PKU75_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82393.1"
                     /translation="MPTPPSSPRRRVQGAVEPVISRALGEGRRRGVTQRAGRFLPGRA
                     RPLTEFTGWTTTEYAAYHALPAGAPAPARRASSTIATRVNALSATLPTEAVLLSRAIT
                     DTAPSAGDDLNPTLADLLERYLPESISAYEVSTRRGRNDSAEQLLMTQLRLLHTVALN
                     IDEAETAHNERDLQIQDRFLRERFANLTPGDLDLTERPVTAATVRSIDAPARGRNKTA
                     TPDARAFLDPERQPVVLFKRATGGAWDLTLRLALPKGQATILGVVQETTAGATNFVHR
                     GNRRLLGARRPTGFKAPQVDLTLQVKLLSPKRFFIYAESAAGKDLTDTVLFFRTDNGS
                     QAELPTALTNHARAPLTVIASAYDTPDGLVLRNESVLFPDLRSACTGFGYGNITWLDR
                     HTPIV"
BASE COUNT          621 a         1391 c         1337 g          487 t
ORIGIN      
        1 gctgactgcc aaccagcgcg acttcacgcg gatccccggg ctggacgtgc tcgactacac
       61 ggagtgaccc gacgttcgcg cgcggaccgg ctctggcgcc ggccgggagt cgaactcgcg
      121 gaagctcggg accagcagcg gcagcagggt gacgaggagg tatgccgtcc cgacgaccca
      181 caggacggga gcgagcccga gcgaggcggc cagcaggccg ccaagcagcc cgccgagcgg
      241 catcagcgac cagcacagcg cggagttcag cgcggagacg cggccgacga gccgttcggg
      301 gatccgctcg aagaccaccg cgcccaggat cgggttgagg aatccgcacg ccaggccccc
      361 gattacgaac acggccacac agacgcacag cggtgacccc agcgccagca cggcgaagcg
      421 cgggagaccg gtgaggaggt aggcgatcac gtaggtgcgg aaccggggca ggcgctcggc
      481 ccagcgtgcc gcggccatcg agctcagcgc cgcgcacgcg gaaaagaccg cgaagaggac
      541 accgatgcct gccgcaccat gaccggagtt gcgcccccat gcgggcatca gcactgccga
      601 ccaggccagg tcgagcagat tggtggcggc gacgatggcg ctcatcccca cgagcacgac
      661 gtcacgacgc agaaagtccc agccacggcg cagcttctgc acgtaaggca ccgggtcgcg
      721 gtccgccaca ggcaccagcg gcggcgagcc ggcggtcagt cccaggatca gtgcgcagac
      781 caggaaggac gcggcatcgg catacagcgc gcccggggcg ccgacccagg cgatcaggcg
      841 tccgcccagg gcggcgccgg ccatcgaggc gatccggtcg atcacgccgt agagcccggt
      901 cacccgcttc agcggcactc ccgcggccgt cgcgaccgcg ggatcagggc ggccttggcg
      961 ccgtcgccgg gcccgcggag cgcgccggcg acacccacca ggaccagcag gaccgggaag
     1021 gagagcccgc cgatcccgtg gagcagcgga atcgccgcca ccacgaccgc gctcgccggg
     1081 tcgcaggaga gggcgaccgc ccggccgccg tagcggtcgg cgaacgggcc gccgaggacc
     1141 tcgaacaaca caagcggggt catctcgacc gccgcgacta ggccggtatg ggcggcggac
     1201 ccggtcgtgg tcagcacgaa ccacggcagc gcgatcatcg agatgcgggt cccggtcagc
     1261 gagatcgcgt tggccaccag gaggccgcca aggggccgcc gactcaccac tgcccgcgct
     1321 cgggcacggc gtcggggtgg aggtaggcgt tgaggttcac gacgaaggtg gctgccgcgg
     1381 cgtcggcgtc ctcggcatac tcctcgatca ccgccaccag ccggtcccgc aggccctggg
     1441 cccgttccgg cgtcagccgc agttgccagt cgctgaaggt gccggcatcg cgccatgggg
     1501 acggcagcag gtcgtgtcgc tcgacggcac gctggagctg ctcggcatac aggatcccga
     1561 ccgcctggac atatgcgccg tgcgtctcgt gctcctccgg cgtgctcagc gccgagctgt
     1621 cggtgaccgt cgactggtga gcggcccgcc accaccgctc ccggccgttc ccgcggccgg
     1681 cgtcctcctc gatgaagccg tgctgggcca gctgacgcag gtggtatgac gtcgcgccag
     1741 tattgagacc gagccgccgc gcgagcgagg tggccgtcgc cggaccctcg aggcgcagca
     1801 gcccgagcat ccgcagccgg gccgggtggg cgagcgcact agtttggaaa gtactctttg
     1861 cattcagcga gttggacgcc atcacggcga tgacgtactg ccgcggcacg cacgatgact
     1921 gctgcgtatt ggacaacaac tcccgcgggg gacgagcgag acggcgatga cgtgcccgag
     1981 cggcgccgtg gtgcccggga cctcgcgtgc tggcgtgggt cggctacgcc cgaggcgggt
     2041 tgcacgctgt ggcaacggtt tcgggatagg tgaggtcggc ccgccacggc tcgaggtccc
     2101 acgtcggctt ggcgttcgcg aagtaggcgt ggcagacgaa ctggtcgtgc aggccgtcgg
     2161 tgtccgcttc cgggtatgcc gcggtgacct ccccccaggc cgcgtccagc gccgctgcgc
     2221 tgaggtggtc gcgcagtgct tcgcgcgggg tgacttcgta ggaggcgccg ccttccgcct
     2281 cgacgagccg cacccccgcg atcaggtcgg gttgaccggg cgggctgctc gacgtgagac
     2341 ggggcgcggt ccgaggcgac gtggcggtcg acccagcact gctgggcgag ttcaccgagc
     2401 tgccggaggc ctgggttggg tgctcctcgc cgcatccggc gagcaggccg gccccagcca
     2461 gcatgccggc ggtgacccgg cagcagcggg tggcccatct catgtccaca tgatgccggt
     2521 cgcgggttag cctgacgcgg tgcccacgcc tccatcgagt cctcggcgcc gcgtccaggg
     2581 agccgtcgaa ccggtgatct cgcgcgccct gggcgagggg cgccgccggg gcgtcaccca
     2641 gcgcgccggc cggttcttgc ccggccgggc ccgtcccctg accgagttca ccggctggac
     2701 gacgacggag tatgcggcat accacgccct gccggccggg gcgccggcgc cggcgcggcg
     2761 ggccagcagc acgatcgcga cccgggtcaa cgcgctcagc gccaccctgc ccaccgaggc
     2821 ggtgctgctc agccgggcga tcaccgacac cgccccgagc gctggcgacg atctgaatcc
     2881 gacgctggcc gaccttctgg aacgttattt accggagagc atttcggcgt atgaggtgtc
     2941 cacgcgccgg ggccgcaacg actcggccga gcagttgctg atgacccagt tgcggctgct
     3001 gcacaccgtc gccctcaata tcgacgaggc cgagaccgcg cacaacgaac gcgatctaca
     3061 gatccaggac cgcttcctgc gcgagcggtt cgccaacctg acgccgggcg acctcgacct
     3121 gaccgagcga cccgtgaccg cggcgaccgt gcgcagcatc gacgcgccgg cccgcgggcg
     3181 caacaagacg gccacgcctg acgcccgcgc cttcctggac ccggaacggc aaccggtcgt
     3241 cctgttcaag cgagcgaccg gcggtgcctg ggatctgacg ctgcgcctgg cgctgcccaa
     3301 ggggcaggcg accatcctcg gcgtcgttca agagacgacc gcgggagcga cgaacttcgt
     3361 ccaccggggc aaccgccgcc tcctcggcgc gcgccggccc accggcttca aggcccctca
     3421 ggtcgacctg acactgcagg tgaaattgtt gtcccccaag cggttcttca tctacgccga
     3481 gagcgccgcc ggcaaggacc tcaccgacac cgtcctcttc ttccgcaccg acaacggcag
     3541 tcaggccgag ctgccgaccg ccttgaccaa ccatgcccgc gccccgctga ccgtcatcgc
     3601 gtccgcatac gacacccccg acggcctggt gctgcgcaac gagtcggtcc tcttccccga
     3661 cctgcgcagc gcctgcaccg gattcggcta cggcaatatc acctggctcg accggcatac
     3721 cccgatcgtc taggtccgcc ctagaaggct cgaccggcat acccccatca tctaggtccg
     3781 ccctagaagt agcgcagcgt cacatacggg accgtgcaca ggatcgtcac tgccgt
//
LOCUS       DAOPJG010000255         3781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_26830_length_3781_cov_7.568975, whole genome shotgun sequence.
ACCESSION   DAOPJG010000255 DAOPJG010000000
VERSION     DAOPJG010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26830_length_3781_cov_7.568975"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKU75_15820"
     CDS             <1..281
                     /locus_tag="PKU75_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPF82394.1"
                     /translation="RRPAPLHLTPRQVLAWEHVAGLTVGQDLSWREVQGRGPAPQFAM
                     GKSYPGFSPLGPYVVTVDEFADKDDLAISCALDGEVLQEGRTSDRRPS"
     gene            317..499
                     /locus_tag="PKU75_15825"
     CDS             317..499
                     /locus_tag="PKU75_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82395.1"
                     /translation="MPDEVIFTSALPHTMTGKRMEVPVKRLLQGVPLEQAVNPSAVDD
                     PGTLDQFVALAREGRG"
     gene            complement(567..1589)
                     /locus_tag="PKU75_15830"
     CDS             complement(567..1589)
                     /locus_tag="PKU75_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82396.1"
                     /translation="MPRTPVPVALTLLLLPLAACESDPPATSATTSSASSAPTTSAPA
                     RTAAMTTKEAATFWKAAPKHLKSAYAAAYGKKYDRSALADVFAEPSLSVFTFASRYKQ
                     AVKDTDKPGTIRFAPEVLYAGAPGSGAESALSVGRGSIGWGEQSAAASAAVWLRRTSP
                     DADWRVAASVPVAMDRLPEAPDPGAAEPSDADRAWARKAVDAIADYLETGRAKGFTPG
                     TLLTNIHKSEKDSGFKVRTTATLFGKKTDDPTAPGGPVSVWRAGSQIVAIGVIERHQV
                     VTDPNGGMLTYKDEIRKLYRLPSTQAKEGSLYQAVTVLITGKPSGKPTVVAAQSGALM
                     PPRLRD"
     gene            1776..2153
                     /locus_tag="PKU75_15835"
     CDS             1776..2153
                     /locus_tag="PKU75_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPF82397.1"
                     /translation="MAQYLIAFNDEWVPPHTPEQIRAKGVASWAVIDEMREAGVFVYG
                     NGALDASTVVCNVESRDGEPVFTDGPYAETKEHLGGFTVVDVPDDAAARYWAGRLAVA
                     LEWPQEVHRFPTMDRPRPDDPRK"
     gene            2159..2545
                     /locus_tag="PKU75_15840"
     CDS             2159..2545
                     /locus_tag="PKU75_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPF82398.1"
                     /translation="MPRYLLSVFGPAERTDYGPYPSKEDMLQAFADTGAFNDRLQREG
                     YFVFADGLEPATTATTVDGQGESPVLTDGPYLETKEHIGGFWVIEAPDLDVALALAAD
                     GSKACRGVIEVRPFQTAESVAALLDS"
     gene            2542..>3781
                     /locus_tag="PKU75_15845"
     CDS             2542..>3781
                     /locus_tag="PKU75_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPF82399.1"
                     /translation="MTSPDVDAALVRAHHDEWARIVAGLARRYGDLDLAEDATAEAFA
                     VAVQRWPDDGIPPNPGGWLMTTANRKALDRLRRESQRDAKHLAAQHLVDDAPPEPVGP
                     VEDDRLRLIFVCCHPALSMQARVALTLRLLGGLTVAEIAHAFLVPETTLAQRITRAKG
                     KIKAARIPYAVPAATDVAERLTGVQAVVYLVFNEGYLASGGDEAVRTPLCDEAIRLGR
                     LLHELLPDDGEVTGLLALMLLTDARRNARVSRTGELITLDEQDRGGWDRGLIAEGHAL
                     VRERLAAVAAGGPAPGRYQLLAAINAVHTDAPTARDTDWSQILALYDRMALIDPSPIV
                     RLNRAVAVTELDGPLVGLAEIDRLTDALDGYHAFHAARADVLRRLGRSGDARAAYDRA
                     IALAGNPAERAYLTRRRDQLA"
BASE COUNT          535 a         1334 c         1355 g          557 t
ORIGIN      
        1 ggcgccggcc cgcgcccctg cacctcacgc cacgacaagt cctggcctgg gagcacgtcg
       61 cgggcctgac cgtcggccag gacttgtcgt ggcgtgaggt gcaggggcgc gggccggcgc
      121 cgcagttcgc gatgggcaag tcctatcccg ggttcagccc gctcgggccg tatgtcgtca
      181 cggtcgacga gttcgccgac aaggacgacc tggccatctc ctgcgccctc gacggcgagg
      241 tgctgcagga gggccgcacc agcgaccggc ggccgagctg aagcagctga tccgggccga
      301 ggtctctcca cggcatgtgc cggacgaggt catcttcacc tcggccctgc cccacacgat
      361 gaccggcaag cgcatggagg tgccagtcaa gcggctgctc cagggcgtgc cgctcgagca
      421 ggcggtcaac cccagcgcgg tcgacgaccc cgggacgctc gatcagttcg tggccctcgc
      481 ccgggagggc cggggctgag tcgccccggg tgcggggcgg tacgccgtgt gcgctctggc
      541 gcgtcatcgg gcacacgtgc ctggcgtcag tcgcgcagtc ttgggggcat cagggcgccg
      601 gactgggccg cgacgacggt gggcttgccc gacggcttgc cggtgatcag gacggtgacc
      661 gcttgataca acgacccctc cttcgcctgg gtggacggga ggcgatagag cttgcggatc
      721 tcgtccttgt aggtgagcat cccgccgttc gggtcggtga cgacctgatg ccgctcgatg
      781 acgccgatgg cgacgatttg cgagcctgcg cgccacacgc tgaccggccc acctggggcg
      841 gtcgggtcgt cggtcttctt gccgaacagg gtggccgtcg tgcgcacctt gaatcccgaa
      901 tccttctcgc tcttgtggat attcgtgagc aaggtgcccg gcgtgaagcc cttggcccgc
      961 ccggtctcga gatagtcggc gatggcatcg acggccttgc gcgcccaggc ccggtcggcg
     1021 tcgctgggtt cggcggcgcc ggggtcgggg gcttcgggga ggcggtccat cgcgacgggc
     1081 accgacgccg caacccgcca gtcggcgtcg ggactggtgc ggcgcagcca cacggcggcc
     1141 gaggcggcgg cgctctgttc cccccagccg atcgatcccc ggccgacgct caacgccgac
     1201 tcggccccgg aacccggcgc gccggcatac agcacctcgg gggcgaagcg gatcgtgccc
     1261 ggcttgtcgg tgtccttgac ggcctgcttg taccggctgg cgaaggtgaa cacggacagg
     1321 ctcggctcgg cgaagacgtc cgcgagcgcc gaccggtcgt acttcttccc gtaggccgcg
     1381 gcgtacgccg acttgaggtg cttcggcgcc gccttccaga acgtggcggc ctccttggtc
     1441 gtcatggcgg cggtccgggc cggcgcggag gtggtgggcg cgctcgacgc ggacgaggta
     1501 gtggcggagg tggccggcgg atcgctctcg cacgccgcca gcggcagcag gaggagggtc
     1561 agggcaacgg ggactggggt gcgcggcata cgacgagcct cccccgggat cgcgatcctg
     1621 tcgcggcgag cgcgaccggg ctgtggataa gtcccgctgc cgaaattcgt gcggatgtgg
     1681 tgtcgtatgg cgtcaggtgc gttcgtcgta tgggtgagcg gccggccggg ccgcgaccga
     1741 cgagcgggcg atggcccgcc tgagaggaga cagggatggc gcagtatctg attgccttca
     1801 acgacgagtg ggtgccaccg catacgcccg agcagatccg ggccaagggg gtggccagct
     1861 gggcggtgat cgacgagatg cgcgaggccg gggtgttcgt ctacggcaac ggcgcgctcg
     1921 acgcctccac cgtggtgtgc aacgtcgaat cccgtgacgg ggagccggtt ttcaccgatg
     1981 ggccgtatgc cgagaccaag gagcacctcg gcgggttcac cgtcgtggac gtccccgacg
     2041 acgcggccgc gcgctactgg gcgggccggc tcgcggtcgc cttggagtgg ccgcaggaag
     2101 tgcaccggtt ccccaccatg gaccggcccc gacccgacga cccgaggaag tgaccaccat
     2161 gccccgatac ctgctgtccg tcttcggtcc ggccgagcgc acggactacg gcccctaccc
     2221 ctccaaggag gacatgctcc aggcgttcgc cgacaccggc gccttcaacg accggctgca
     2281 acgcgagggc tacttcgtct tcgccgacgg gctcgagccg gccacgaccg ccaccacggt
     2341 cgacgggcag ggcgagtccc cggtcctgac cgacgggccc tatctggaga ccaaggaaca
     2401 catcggcggg ttctgggtga tcgaggcccc cgacctcgac gtcgccctgg ccctcgccgc
     2461 cgacggctcg aaagcgtgcc gcggcgtcat cgaggtgcga cccttccaga ccgcggaatc
     2521 cgttgcagca ctgctggatt cgtgacttct cccgacgtcg atgcggcgct cgtccgcgct
     2581 caccacgacg agtgggcacg gatcgtcgcg gggctggcgc gtcggtatgg cgatctcgac
     2641 ctcgccgagg acgcgacggc cgaggcgttc gccgtggcgg tgcagcggtg gccggacgac
     2701 ggcataccgc ccaatcccgg aggctggctc atgacgaccg ccaaccgcaa ggcgctcgac
     2761 cggctgcggc gggagagcca gcgcgacgcg aaacaccttg cggcgcaaca tcttgtcgat
     2821 gacgcgcccc cggagccggt cggcccggtc gaggacgacc ggctccgcct gatcttcgta
     2881 tgctgtcacc ccgccttgtc gatgcaggcg cgcgtcgcgc ttaccctccg cctgctcggc
     2941 gggttgacgg tggccgagat cgctcacgct ttcctggtgc cggaaaccac tctggcacaa
     3001 aggatcacgc gcgcgaaggg gaagatcaag gccgcgcgca ttccgtatgc ggtgcccgcc
     3061 gccaccgatg tcgccgagcg gctcacgggg gtccaggcgg tcgtctatct cgtgttcaac
     3121 gagggctatc tcgcgagcgg cggcgacgag gcggtgcgga ctccgttgtg cgacgaggcg
     3181 attcggctgg gacggctgct ccacgaactg ttgcccgacg acggcgaggt caccggcctg
     3241 ctcgcgctga tgctgctgac cgacgcccgc cgcaacgcac gcgtctcccg cacgggcgaa
     3301 ctgatcaccc tcgacgagca ggaccgcggc ggctgggacc gggggctgat cgcggaagga
     3361 catgcgctgg tgcgggagcg gttggccgcc gtcgccgccg gcgggccggc gcccgggcgc
     3421 taccaactgc tcgccgcgat caacgccgtc cacaccgacg cccccaccgc gcgcgacact
     3481 gactggtccc agatcctcgc gctctatgac cggatggccc tgatcgaccc ctccccgatc
     3541 gtgcggctca accgcgccgt ggcggtgacc gaactcgacg gccccctcgt cggtctcgcc
     3601 gagatcgacc ggctcaccga cgccctcgac ggctatcacg cgttccacgc cgcccgggcc
     3661 gacgtgctgc gccggctcgg tcgcagcggc gacgcccgcg cggcatacga ccgggcgata
     3721 gccctcgccg gcaaccccgc cgagcgcgcc tacctcaccc gccgtcgcga ccaactcgcc
     3781 c
//
LOCUS       DAOPJG010000256         3776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_26877_length_3776_cov_4.820747, whole genome shotgun sequence.
ACCESSION   DAOPJG010000256 DAOPJG010000000
VERSION     DAOPJG010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26877_length_3776_cov_4.820747"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKU75_15850"
     CDS             <1..627
                     /locus_tag="PKU75_15850"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPF82400.1"
                     /translation="ERARIAREMHDIVAHSLSVVVVQSDGAAYAAEHASSWDRDQAAH
                     ALKTIGETARSALAETRRLVGVLRQEGDAEDYAPTSSLAQLDELIDGTRQAGLMVELD
                     ERGDRRALPREGDLAAYRIVQESLTNVLRHAGPGAAARVTVDYGEPVRIEVRDNGSGT
                     GSGDGQGNGLIGMRERIHQVGGSFVAGPAPGGGWRVQALIPTGTTGEQ"
     gene            627..1340
                     /locus_tag="PKU75_15855"
     CDS             627..1340
                     /locus_tag="PKU75_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPF82401.1"
                     /translation="MSESVPQPGQSPIRVFLVDDQELVRAGFRMVLDAQDDMTVVGEA
                     GDGQAALDQLASVPADVVLMDIRMPRMDGVAATKALCGKGSGATRVVVLTTFDLDEYA
                     FAALKAGAAGFLLKDAGPADLLAAIRAVHRGDSVIAPSTTRRLLDHFAGALPDSPGAP
                     AAADRSRLAPLTAREVEVLTAVGRGLTNAEIAATLFMAEATVKTHIGRVLHKLDLRDR
                     VQMVLLAYETGLVEVRPRS"
     gene            1422..2186
                     /locus_tag="PKU75_15860"
     CDS             1422..2186
                     /locus_tag="PKU75_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF82402.1"
                     /translation="MTTTTVRHPDAPATTAAVRARQLTKIYGSGSAAVPALRGVTTSF
                     ARAEFTAIMGPSGSGKSTLMHCLAGLDAPTDGQVWLGETELSALGDDALTRLRRESVG
                     FVFQAFNLVPTMTAAQNISLPLRLAGKRPDQGWLGQVVETLGLADRLAHRPSELSGGQ
                     QQRVAVARALLTRPDVLFADEPTGALDSRTSAEMLHFLRASVQDLGQTIVMVTHDPGA
                     AAYADRVVLLADGVVAGEIVSPTADSVLDALRGLGA"
     gene            2191..>3776
                     /locus_tag="PKU75_15865"
     CDS             2191..>3776
                     /locus_tag="PKU75_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPF82403.1"
                     /translation="MLDVALASLRTQARRFIAPGLAILLGVAFLTTSLVLGTTAVKGL
                     SDSIAGAGAGFSAVISPPSREVGIMPASTLAKVDAVPGVQDVHEVRFAAVRPSGDSSR
                     VVFLTTPPAAGSRSHLVAGRLPASDSEVAITSTTRDSKNLRIGDHVDYAGADGPHRFT
                     VVGVIDTAGDFVFSGGGTYAFTTSAAVAAVSGEDDFAELDVTATAGTDPKTLVAALNT
                     ALGDTAMARTGADHAAYLRAQVTGGTDVLRLLLLGFAGIALFVSALVIANTFAILAAR
                     RTRETALLRCVGATRRQLLRAMSLEALVVGFAASLAGVATGAALAWAGMRTLAAFDST
                     EALAGSGLVVRPLDLLGPLLVGTGVTVLASLAPLRRATQVTPLAALRPDLGAPARART
                     SGSRLGIGGTAALAGAGLLAFGGARHQLLAGLIGGAVSFVGVVLLARVFVPALIRGLG
                     SPVQRLAGVPGELAVDNAVRNRSRTANTATALLVGVTLISTMTVGAATLLRSVDTLLD
                     HKMAVDVVASALPDAALPARV"
BASE COUNT          485 a         1373 c         1350 g          568 t
ORIGIN      
        1 gagcgcgcgc ggatcgcccg ggagatgcac gacatcgtcg cgcacagcct ctccgtcgtc
       61 gtcgtgcagt ccgatggtgc tgcgtatgcc gccgagcacg cgtcctcatg ggaccgcgac
      121 caggcggcgc acgccttgaa aaccattggg gagacggcgc gttcggctct cgccgagacc
      181 cggcgactgg tcggagtcct gcgtcaggag ggggatgccg aggactacgc gccgacgagt
      241 tcgctcgcgc agttggacga gttgatcgac gggacgcgac aagccgggtt gatggtcgag
      301 ctcgacgaac gcggcgaccg ccgcgcgttg ccccgcgagg gcgacctggc ggcataccgc
      361 atcgttcagg agtccctgac gaacgtcctg cgtcacgccg ggcccggggc ggccgcgcgc
      421 gtgacggtcg actacggtga gccggtgcgg atagaggtgc gcgacaacgg ttccggcacg
      481 ggctccggcg acggacaggg caacggcctg atcgggatgc gggagcgcat ccaccaggtg
      541 ggcgggagct tcgtcgccgg ccccgcgccg ggtggtggct ggcgggtgca ggcgctcatt
      601 ccgacgggaa cgacagggga gcagtgatga gcgagtccgt gccgcagccg ggccagagcc
      661 cgatccgggt cttcctcgtc gacgaccagg aactcgtccg tgccggtttc cgtatggtgc
      721 tcgacgccca ggacgatatg acggtcgtcg gcgaggcggg agacggtcag gcggccctcg
      781 accagctggc ttccgtcccg gcggacgtcg tcctcatgga catccggatg ccgcggatgg
      841 acggcgtggc tgccacgaag gcgttgtgcg gcaagggaag cggcgccacg cgcgtggtcg
      901 tcctgacgac attcgacctc gatgaatacg ccttcgcggc gctcaaggcc ggtgccgccg
      961 gcttcctgct caaggacgcc ggcccggctg atctgctggc cgcgatccgg gccgtccacc
     1021 ggggggactc ggtcatcgcc ccctcgacga cacgccggct gctcgaccac ttcgccggcg
     1081 cgttgccgga ctcgcccggg gcgcccgccg cggcggatcg ctcccgcctg gcgcccctga
     1141 cggcccgcga ggtcgaggtg ctcacggccg tcgggcgggg gctgacgaat gccgagatcg
     1201 ccgccacgct cttcatggcc gaggcgacgg tgaagaccca catcggccgg gtcctgcaca
     1261 agctcgatct gcgggatcgg gtccagatgg tgctgctcgc ctacgagacg gggctcgtcg
     1321 aggtgcgacc gcggtcgtag tcccgcggtc gcagccgcgc tgcccaggtc cagaccgcgg
     1381 tccgatgcgg cgaacccggt tgtggcggaa tcgttgtggg catgacgaca acaaccgtcc
     1441 gccacccgga tgcgcccgcc acaaccgccg ccgtccgcgc ccggcagctg accaagatct
     1501 acggcagcgg gtccgccgcc gtgcccgcgc tgcgtggtgt caccacctcc ttcgcccgcg
     1561 cggagttcac cgcgatcatg gggccctccg gttcggggaa gtccacgctc atgcactgcc
     1621 tggccggact ggacgcaccg accgatggcc aggtgtggct cggggagacc gaactcagcg
     1681 cgctcggcga cgacgccctc acccggctgc gccgcgagag cgtcgggttc gtcttccagg
     1741 cgttcaacct ggtcccgacc atgaccgcgg cccagaacat ctcgctgccg ctgcggctgg
     1801 ccggcaagcg gccggaccag ggctggctcg ggcaggtggt ggagacgctg ggcctcgcgg
     1861 accgactcgc gcaccggccg agcgaactct ccggtgggca gcagcagcgg gtcgctgtcg
     1921 cccgcgccct gctgacgcgt cccgacgtgc tcttcgccga cgagccgacc ggcgccctcg
     1981 actcgcgcac ctcggcggag atgctgcact tcctgagggc ctccgtgcag gacctcggcc
     2041 agacgatcgt catggtgact cacgaccccg gcgccgcggc atacgccgac cgcgtcgtgc
     2101 tgctggcgga tggtgtcgtc gcgggcgaga tcgtctcgcc cacagcggat tcggtgctcg
     2161 acgcgttgcg cggtctgggg gcctgacgcg atgttggacg tcgccctcgc gtcactgcgc
     2221 acccaggctc gccggttcat cgcccccggg ctcgcgatcc tgctcggagt ggctttcctg
     2281 accaccagcc tggtgctggg aacgacggcc gtcaaaggct tgtccgactc catcgccggc
     2341 gcgggcgcag gcttttccgc cgtgatctcc ccaccgtcac gggaggtcgg gatcatgccc
     2401 gcgtcgaccc tcgccaaggt cgatgcggtc cctggagtcc aggacgtcca cgaggtccgc
     2461 ttcgccgcgg ttcgcccgtc gggcgactcc agccgcgtcg tcttcctcac gacgcctccg
     2521 gccgcggggt cccgctccca cctcgtggcg ggccgcctgc cggcgtcgga ctcggaggtg
     2581 gccatcacca gcacgacccg ggacagcaag aacctccgca tcggagatca cgtggactac
     2641 gccggcgccg acgggccaca ccgcttcacc gtcgtgggcg tcatcgacac cgccggcgac
     2701 ttcgtcttct ccggtggtgg gacctacgcg ttcacgacga gcgcggcggt cgccgccgtc
     2761 agcggggagg acgacttcgc cgaactcgac gtgaccgcaa ccgccgggac ggaccccaaa
     2821 acccttgtcg cggcgctgaa cacggcgctg ggcgacacgg cgatggcccg cactggggcg
     2881 gaccacgcgg catacctgcg ggcgcaggtg accggcggca ccgacgtgct gcggctgctg
     2941 ctcctgggct tcgccggcat tgccctcttc gtctccgcac tggtcatcgc caacaccttt
     3001 gcgatcctgg ccgcccgccg cacccgggag accgccttgt tgcgatgcgt cggcgccacg
     3061 cgccggcaac tcctgcgcgc gatgtcgctc gaggctctcg tcgtcggctt cgcggcctcg
     3121 ctcgcgggcg tggcgacggg ggcggcgctg gcctgggcgg gtatgcggac actggccgcc
     3181 ttcgacagca cggaagcgct cgccgggtcc ggcctggtgg ttcggcctct ggacctgctc
     3241 gggccgctgc tcgtcggcac cggcgtcacg gtgcttgcga gcctggcgcc gctgcgccgc
     3301 gccacccagg tgacgccgtt ggcggcgctg cgccccgacc tcggggcgcc ggctagggca
     3361 cgcaccagcg gctcgcgtct cggcatcggc gggaccgccg ccctcgcggg agcggggctg
     3421 ctggccttcg gcggcgccag gcaccaactc ctcgccgggc tgatcggcgg tgccgtgtcc
     3481 ttcgtgggcg tcgtgctgct cgcgagagtc ttcgtgcccg cgctgatccg cggactcggt
     3541 tcacccgtgc agcgcctggc gggggtgccg ggggaactgg ctgtcgacaa tgcggtccgc
     3601 aatcgctccc ggacggcgaa cacggcgacc gccctgctcg tcggtgtcac gctgatctcc
     3661 accatgaccg tgggggcggc caccctgctc cgctcggtcg acaccctgct cgaccacaag
     3721 atggccgtcg atgtcgtcgc ctcggccctc cctgacgcgg cgctgccggc ccgggt
//
LOCUS       DAOPJG010000257         3733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_27310_length_3733_cov_8.660957, whole genome shotgun sequence.
ACCESSION   DAOPJG010000257 DAOPJG010000000
VERSION     DAOPJG010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27310_length_3733_cov_8.660957"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..862
                     /gene="truA"
                     /locus_tag="PKU75_15870"
     CDS             35..862
                     /gene="truA"
                     /locus_tag="PKU75_15870"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPF82404.1"
                     /translation="MRIRIDLAYDGTDFAGWAAQPGQRTVEGELSTALTTLLRAPEPV
                     RLVVAGRTDAGVHARGQVAHADVDAAAYAALPGRSSRSPEAAAATRLRGILPPDIVVR
                     QVSPAPAGFDARFSATHRRYCYRLCDDPAARDPLRLRDTVLVADPLDAAAMHHAAQGL
                     LGLHDFAAFCKKREGATTTRTLLEYSWTRTAEGVLEARVVADAFCHSMVRALVGVVMP
                     VGLGRAGVSFPREVMIAGIRDPRVKVMPAHGLALEEVGYPPPHLMAARAEEARARRD"
     gene            996..2858
                     /locus_tag="PKU75_15875"
     CDS             996..2858
                     /locus_tag="PKU75_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HPF82405.1"
                     /translation="MSAAESMALAGEFPQQTHEDWQRLVAAVVNKSRAEDAKLTPEDA
                     EASLRTTLSGGLVVDPLYQRSADAAAIGVPGAMPFTRGTGLRPADQPWDVRQLHDDPD
                     ANISRAAVLDDLEHGVTSVWVHVGADGVAPGDLAAVLADVKLDMAPVVVSSWDAQGDA
                     AAALQSVLQGSPDVHGKGNYGLDPLGAAARTGAAADLAGLAGAVESLSGIPGMRAITV
                     DARVYRDAGASAVDEVAYAVATGVAYLRALADAGVDAPTAFGQIDFRVSATADQFLTI
                     AALRALRRLWARVGEVCGVPEPERGARIHAVTSQRMFTRDDPWVNVLRSTLATFGACA
                     GGADAITVLPYDTVAGLPEKFSRRLARNTQIVLADEANVGRVADPAGGSYYVEHLTAD
                     IAHGAWSAFQEIEKAGGMAAALASGSVAARIRSTRDEDGARLATRRLPITGVSMFPLT
                     GEQPVARRQRAALAVSDKALAPQRDSVLYESLRNRAGAYAAAHGSAPGVALVALGARR
                     DFGARETFASNLLAAGGVTATLYEGESAAADAAAAKPEVVVIASSPKGYAAHAAAAVE
                     ALRSAGIPRVLVAGRARELGETSVDGEIYDGMDVVAFLSDTLDRLGAPAEGAAQ"
     gene            2855..>3733
                     /locus_tag="PKU75_15880"
     CDS             2855..>3733
                     /locus_tag="PKU75_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPF82406.1"
                     /translation="MTTIPDFTAIDLGDGASADAPVPQGESWSTPEGIEVAPVYTEAD
                     LQGLDFLDTVPGAPPFLRGPYPTMYVNQPWTIRQYAGFSTAEESNAFYRRNLAAGQKG
                     LSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFSGIPLDQMSVSMTMNGA
                     VIPILALYVVAAEEQGVAPDKLTGTIQNDILKEFMVRNTYIYPPGPSMRIVSDIFAFT
                     SEKMPRFNSISISGYHMQEAGATQDLELAYTLADGVEYIRAGKAVGLDVDTFAPRLSF
                     FWAIGMNFFMEVAKMRA"
BASE COUNT          507 a         1342 c         1306 g          578 t
ORIGIN      
        1 cgagcactga gcccactcgt cgccggtgag tgggctgcgg atccggatcg atctcgcgta
       61 tgacgggacc gacttcgccg ggtgggccgc ccagccggga cagcggacgg tcgagggcga
      121 gctttccact gcgctgacca cgctgctgcg cgcgccggaa ccggtccggc tcgtcgtcgc
      181 cggccgcacc gacgcggggg tgcacgcccg cgggcaggtc gcccacgccg acgtcgatgc
      241 cgcggcgtat gccgccctcc cgggccgctc gtcccgctcg ccggaggccg cagcggcgac
      301 ccggctgcgc ggcatactgc cgccggatat cgttgtgcgc caagtcagtc cggcgcccgc
      361 cggcttcgac gcccgcttct cggccacgca ccgtcgctat tgctaccggc tgtgcgacga
      421 cccggccgcc cgcgacccgc tgcgtctccg ggacaccgtt ctcgtcgccg atcccctgga
      481 cgcggccgcc atgcatcacg cggcgcaggg gctgctgggg ctgcacgact tcgcggcctt
      541 ctgcaagaag cgagaggggg ccacgacgac gcgcacgctc ctggagtatt cgtggacccg
      601 taccgccgag ggcgtcctgg aagcgcgcgt cgtcgccgat gccttctgcc actcgatggt
      661 ccgcgcgctc gtgggcgtcg tcatgccggt cgggctgggg cgggccgggg tgagctttcc
      721 gcgggaggtc atgatcgccg gcatccggga cccacgggtg aaggtcatgc cggcgcacgg
      781 gcttgcgctc gaagaggtcg gctacccgcc gccgcacctg atggcggcgc gcgccgagga
      841 ggcccgggcc cgccgggact gacgctgtgc ggaacctctc gctccaaatg gtgggtagct
      901 tcgcgtcacg tcgataggct ggtcgaagcc cggcgaggcc gccgcacgtg cgccctcgcc
      961 gcttttgtgc aaaccggttc gtcaaaggag cgcgcatgtc cgccgctgag tcgatggccc
     1021 tcgcggggga gttcccgcag cagacgcacg aggattggca gcgcctggtc gcggccgtgg
     1081 tcaacaagtc ccgtgccgag gacgccaagc tcacccccga ggacgccgag gcgtcgctgc
     1141 gcaccacatt gtccggtggc ctggtcgtcg acccgctcta ccagcggtct gcggacgctg
     1201 ctgccatcgg tgtcccgggc gccatgccgt tcacccgcgg caccggcctg cggccggccg
     1261 accagccgtg ggatgtccgg cagttgcatg acgacccgga cgcgaacatc tcgcgggctg
     1321 ccgtcctcga cgacctcgag cacggcgtga cgtccgtgtg ggtgcacgtc ggcgccgatg
     1381 gcgtcgcgcc cggcgacctc gcggcggtcc tcgcggacgt caagctcgac atggctcccg
     1441 tcgtcgtgtc ctcgtgggat gcgcagggcg acgccgccgc agcgctccag agcgtgctgc
     1501 aggggtcccc ggacgtccac ggcaagggca actatggcct cgacccgctc ggcgcggcgg
     1561 cccgcaccgg cgccgcggcc gacctcgcgg gtctggccgg agccgtcgag tcgctgtccg
     1621 gcatccccgg catgcgcgcc atcaccgtcg atgcccgggt ctatcgcgac gccggcgcca
     1681 gtgctgtcga cgaggttgcg tatgccgtcg ccaccggagt cgcgtatctg cgagcgctcg
     1741 cggacgcggg ggtggacgcg ccaacggcgt tcgggcagat cgacttccgc gtctctgcga
     1801 ccgccgatca gttcctcacg atcgccgcgc tgcgggcgct gcgccggctg tgggcgcgcg
     1861 tgggggaggt atgcggcgtc cccgagcccg agcgtggcgc gcggatccac gcggtcacca
     1921 gccagcggat gttcacccgg gacgacccgt gggtcaacgt gctgcgcagc accttggcga
     1981 ccttcggtgc gtgcgccggt ggcgcggacg cgatcacggt gctgccctat gacacggtcg
     2041 ccggactgcc ggagaagttc tcccgccggc tggcccgcaa cacccagatc gtcctcgccg
     2101 acgaggccaa cgtcggccgt gtcgccgacc cggccggcgg ttcctactat gtcgagcacc
     2161 tcaccgcgga catcgcccac ggggcgtggt ctgccttcca ggagatcgag aaggccggcg
     2221 gtatggctgc ggctctcgcg tccggctcgg tcgctgcccg aatccggtcg acccgcgacg
     2281 aggacggcgc acgtctggcc acccggcgcc tgccgatcac cggcgtgagc atgttccccc
     2341 tgaccggcga acagccggtg gcgcgccggc agcgcgccgc tctggctgtt tctgacaaag
     2401 cgcttgcgcc gcaacgggat tctgtcctgt atgagagtct gcggaaccgg gccggagcgt
     2461 atgccgcggc gcacgggtcc gcgccgggcg tcgccctcgt cgcgctgggc gcgcgtcgcg
     2521 acttcggtgc ccgcgagacg ttcgcgtcca acctcttggc ggccggcggc gtgaccgcaa
     2581 ccctctacga gggcgagtcg gcggcggccg atgcggctgc ggccaagccg gaggtggtcg
     2641 tgattgccag ctctcccaag ggttatgcag cgcacgcggc ggcggcggtc gaggccctgc
     2701 gcagcgccgg cataccgcgt gttctcgtcg ccgggcgagc ccgggaactc ggcgagacat
     2761 ccgtcgacgg tgagatctac gacggcatgg acgtcgtcgc cttcctgagc gacacgctcg
     2821 acagactcgg cgccccggcg gaaggagccg cgcaatgacc acgatccccg acttcaccgc
     2881 tatcgatctc ggcgacggcg cgagcgcgga cgcccctgtg ccgcagggtg aatcctggtc
     2941 caccccggag ggcatcgagg tcgcgcccgt ctacaccgag gcagacctcc aggggctcga
     3001 cttcctcgac accgtgccgg gtgcgccgcc gttcctgcgg gggccctatc cgacgatgta
     3061 cgtcaaccag ccgtggacga tccggcagta tgccgggttc tccactgccg aggagtccaa
     3121 cgccttctac cggcgcaatc tcgctgcggg acaaaagggt ttgtcggtcg ccttcgacct
     3181 ggcgacccac cgcggctacg actccgacca cccgcgcgtc gccggcgatg tcgggatggc
     3241 cggtgtcgcg atcgactcga tctatgacat gcgcacgctc ttcagcggga ttccgctgga
     3301 ccagatgtcg gtgtcgatga cgatgaacgg tgcggtcatc ccgatcctcg cgctgtatgt
     3361 cgtcgcggcc gaggagcagg gcgtcgcgcc cgacaagctg accgggacca tccagaacga
     3421 catcctcaag gagttcatgg tccgcaacac ctatatctat ccgccgggcc cgtcgatgcg
     3481 gatcgtctcc gacatcttcg cgttcacgtc ggagaagatg ccgcggttca actcgatctc
     3541 catctccggc tatcacatgc aggaggccgg cgcgacgcag gatctcgagc tcgcctacac
     3601 cctggccgac ggcgtcgaat acatccgggc cggcaaggcc gtcgggctgg acgtcgacac
     3661 gttcgctccg cggctgagct tcttctgggc catcgggatg aacttcttca tggaggtcgc
     3721 caagatgcgc gcg
//
LOCUS       DAOPJG010000258         3708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_27585_length_3708_cov_7.398850, whole genome shotgun sequence.
ACCESSION   DAOPJG010000258 DAOPJG010000000
VERSION     DAOPJG010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27585_length_3708_cov_7.398850"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKU75_15885"
     CDS             complement(<1..322)
                     /locus_tag="PKU75_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPF82407.1"
                     /translation="MATFVFDFSEGNKDQKDLLGGKGANLAEMVNMGLPVPPGFTIST
                     EACKAYLKDGKSPDALRVEVTQALRKTEDQIGRHFGADKEPLLLSVRSGAKFSMPGMM
                     ETVLN"
     gene            563..1558
                     /locus_tag="PKU75_15890"
     CDS             563..1558
                     /locus_tag="PKU75_15890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPF82408.1"
                     /translation="MPRSWTGQEVAEVVAAHPEWLGAPLSAGEPHRVGRGESYEAWLP
                     APAGPLVRFARRPIGELPADPAEEVAGMRLAPQGVGPRPIGADVLWVDDEQYPVVAQE
                     FVPGRVLAPIEWTGALRRVLAAQLATLHTVTYPRPGSVRGVSQALDPEPGRLDFAAEV
                     DGIIDHWAPRLPGAPGEAWAALCGPMQRYAAEVAPEIAALQEFSLIHGDPVLTNIVVD
                     GNRPVLIDWEWTQIGDPARDLGFIGGAVHAGDWYAALTDKQITDLLRAYVSAGGRGEV
                     VALRRRRDAWLAAEGLAVLAYLWWVDADPAQAITPANRSAAADLQRTVAAYLAAW"
     gene            complement(1581..2474)
                     /locus_tag="PKU75_15895"
     CDS             complement(1581..2474)
                     /locus_tag="PKU75_15895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPF82409.1"
                     /translation="MTAARGVVVGYDGSPSSEVALTWAIEEARRSGLPLTLVHTAEVD
                     LAVYGTGVGGGDWAYARSDVVGDSDSVTRAKEALGEDLVTVHRTLGNAARHLVEASHD
                     ANLVVTGTRGHGHLTSVLLGSTAYAVAAHAHCPVVVVRVPPRGPTQPVRPDADHPVVV
                     GVDDLAPSGAALEAAAEVAERSGAPLRVVRAVEYHAFIPTIASDGATVAEYDEALRQE
                     ALTMVADAAAKVREKHPDLVIEEAALDGAPAFVLGRESHKAGLVVVGSRGRGGFAGLL
                     LGSTSHALLHEAACPVMVVRS"
     gene            2644..3222
                     /locus_tag="PKU75_15900"
     CDS             2644..3222
                     /locus_tag="PKU75_15900"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPF82410.1"
                     /translation="MTRPPFALLVTGAPGAGKSTVGAELARRLRAALIDLDPATEALT
                     QVVAGLLTVDDLDDPRLANATRAARYSTIEALAVESLRVGTDVVLVAPFTAERRDPAA
                     WSALRGRLESAGARVVLVWLRITPAEIARRVAQRGAQRDLAKGDPGWIGRLDLTAPVV
                     PHLVADASLQPEQAAESVLAQLDSPQTQRNLC"
     gene            complement(3314..>3708)
                     /locus_tag="PKU75_15905"
     CDS             complement(3314..>3708)
                     /locus_tag="PKU75_15905"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPF82411.1"
                     /translation="LGVQYSHNDPSTGARLVSAALQKDPDIVGIFATNLFAAEGSATG
                     VRQAGKTGITIVGFDAGPNQIKALEEDTVQALVAQQPNTIGMDGVEQANAALTGGTVE
                     KKIQTGFTIITKDNLSGDGGKAAYKSKC"
BASE COUNT          545 a         1262 c         1298 g          603 t
ORIGIN      
        1 cgttgaggac ggtctccatc atgcccggca ttgagaactt cgctcccgag cgcacggaca
       61 ggagcagcgg ctccttgtcg gcgccgaagt ggcgcccgat ctggtcctcg gtcttgcgca
      121 gtgcctgggt gacctcgaca cgcaacgcgt cgggggactt gccgtccttg aggtaggcct
      181 tgcaggcctc cgtggagatg gtgaagccgg gcggaaccgg cagccccatg ttgaccatct
      241 cggcaaggtt ggcacccttg ccgcccaaga gatccttctg gtccttgtta ccttcgctga
      301 aatcaaaaac gaatgtcgcc atgatcgcct ccttgcgtcg actcatgctt acacccctcc
      361 caggccccca ccgaccaaaa gaccagcatg aggaacgcag gtcgacctgc tgacataccc
      421 ccgtccatcc ggcctgccat gcgaaaatga ttgggggagt gcgttatcgc cgtcgcaaca
      481 ggcgatagtg aacaattggt gaccggatcc gatggtgaga cggctcgtga tcaatcagac
      541 aggacgggag ggagccgccg gtatgccgcg ttcctggacc gggcaggagg tcgccgaggt
      601 ggtggcggcc caccccgagt ggctgggagc tcctctttcc gctggggagc cgcatcgcgt
      661 gggcaggggc gagtcatacg aggcgtggct ccccgctcct gcagggccgc tggtgcgttt
      721 cgcccgccgt ccgatcggcg agctccccgc ggatcctgcg gaagaggtcg ccggtatgcg
      781 gctcgcccct caaggcgttg gcccgcgccc gatcggggcg gacgtgctgt gggtcgacga
      841 cgagcagtac ccggtggtcg cccaggagtt cgtgcctggt cgcgtcctcg cgccgatcga
      901 gtggaccggc gcgctccggc gcgtgctggc agcgcaactg gcgacgctgc ataccgtgac
      961 ctatccgcgt cccggttccg tgcgcggggt gtcgcaagcc ctcgatcccg agccgggacg
     1021 gctcgacttc gcggccgagg tcgacgggat catcgaccac tgggctcccc ggctccctgg
     1081 tgcccccggt gaggcgtggg ctgcgctctg cggtcccatg cagcgctatg ccgccgaagt
     1141 cgcccccgaa attgctgccc tgcaagagtt ttcgctcatt cacggtgacc cggtgctcac
     1201 caacatcgtg gtcgatggca atcggccggt gctcatcgac tgggaatgga cccagatcgg
     1261 cgatcccgcc cgcgacctgg ggttcatcgg tggggcggtt cacgcggggg actggtatgc
     1321 ggcgctcacc gacaagcaga tcaccgacct gctgcgggcg tacgtgagcg ccggcgggcg
     1381 cggcgaggtg gtcgccttgc ggcggcgacg cgacgcctgg ctggccgccg aaggcttggc
     1441 agtgcttgcc tatctgtggt gggtcgacgc cgaccctgcc caggcgatca ccccagccaa
     1501 tcgctccgcc gccgccgacc tgcaacggac cgtcgcggca tacctcgccg cctggtgacc
     1561 ggcgagcacg aggctcgggt tcagctgcgg accaccatca cggggcacgc ggcctcgtgc
     1621 aggagcgcgt gcgaggtgga tccgaggagg agcccggcga agccgccccg gccgcgggag
     1681 ccgacgacga ccagcccggc cttgtgcgat tcgcggccga ggacgaacgc cggggcgccg
     1741 tcgagcgccg cctcctcgat cacgagatcg gggtgcttct cgcggacctt ggcggccgcg
     1801 tcggcgacca tcgtgagcgc ctcttggcgt agcgcctcgt catactccgc gaccgtggcc
     1861 ccatcgctgg cgatcgtggg aatgaaggcg tgatattcga cggcccgtac gacgcgcagc
     1921 ggagcgcccg accgctcggc gacctcggca gccgcctcca gtgcggcacc ggacggcgcg
     1981 aggtcgtcga cgccgacgac gaccgggtgg tcggcgtcgg gccggaccgg ctgcgtcggc
     2041 ccccgcggcg ggacccgcac cacgacgacg gggcaatgtg cgtgcgccgc aacggcatac
     2101 gcggtggacc cgagcaggac cgacgtcagg tgcccatgcc cgcgcgtgcc cgtgacgacg
     2161 aggttggcgt catgactggc ctccacgaga tgcctggcgg cattgcccag cgtccggtgc
     2221 accgtgacga ggtcctcgcc cagggcctcc ttggcccggg tcacggagtc tgagtctccg
     2281 acgacatcgg atcgcgcgta ggcccaatca cccccaccca cgcccgtgcc gtagacggcc
     2341 aggtccacct cggcggtatg gaccagtgtc agcggcagac cggatctgcg ggcttcctcg
     2401 atggcccagg tcagggccac ctccgacgac ggcgaaccgt catacccgac gacaactcca
     2461 cgtgctgcgg tcatggcaac tgccctcttt ctgcccttcc accgtacgcc tgacgcaccc
     2521 gacaaaacag gggagagacg tgtctgctgc gtctcccgtc gagatgtcga ttcgacccgt
     2581 cgagatgcca cttcgacggg gaatccggcg gggcgggcgg ggtcgacctg gcaggctagc
     2641 cacatgaccc ggcccccctt cgccctcctg gtcaccgggg cccccggtgc gggcaagtcg
     2701 accgtcggcg cggagctggc gcgccggctg cgggccgcgc tgatcgacct cgacccggcg
     2761 acggaagcgc tcactcaggt cgtggccggc ttgctcaccg tagacgatct cgacgacccg
     2821 cggctggcga atgccactcg ggcagcacgc tattcgacga tcgaggcgct ggcggtcgag
     2881 tcgctgcggg tgggcacgga cgtcgtgctc gtggcgccct tcacggcgga gcggcgcgac
     2941 ccggccgcct ggtcggcgct gcgcgggcgc ctggagtccg cgggcgcgcg ggtggtgctg
     3001 gtatggctgc gcatcacccc cgccgagatc gctcgccggg tcgcgcaacg cggggcgcag
     3061 cgggatctcg ccaagggcga tcccggctgg atcgggcgtc tcgacctcac cgccccagtg
     3121 gtgccgcacc tcgtggcaga tgcttcactg cagcccgagc aggccgcgga aagcgttctg
     3181 gcccagctgg attcgcccca aacacaacgt aacctctgct agcagaggtt agtcgaatct
     3241 gcacacctag cacggtgtgc agattcgacc agggtctgct agcagaggtt aggttgacct
     3301 ggtgggggct aggtcagcac tttgacttgt aggcggcctt gccaccgtca ccggacaggt
     3361 tgtccttggt gatgatggtg aagccggtct ggatcttctt ctcgacggtg ccgccggtca
     3421 acgcagcgtt ggcctgctcg acaccgtcca tgccgatcgt gttgggctgc tgggcgacga
     3481 gggcctggac ggtgtcctcc tccagcgcct tgatctggtt ggggccggcg tcgaaaccga
     3541 cgatggtgat cccggtcttg ccggcctgcc ggacaccggt cgcggagcct tcggcggcga
     3601 agaggttcgt cgcgaagatg cccacgatgt cggggtcctt ctgcagggcg gcgctgacca
     3661 ggcgggcgcc ggtcgagggg tcgttgtggc tgtactggac gccgaggt
//
LOCUS       DAOPJG010000259         3668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_27987_length_3668_cov_7.060614, whole genome shotgun sequence.
ACCESSION   DAOPJG010000259 DAOPJG010000000
VERSION     DAOPJG010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27987_length_3668_cov_7.060614"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..146)
                     /locus_tag="PKU75_15910"
     tRNA            complement(74..146)
                     /locus_tag="PKU75_15910"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Glu,seq:ttc)
     gene            complement(331..1386)
                     /locus_tag="PKU75_15915"
     CDS             complement(331..1386)
                     /locus_tag="PKU75_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPF82412.1"
                     /translation="MVALVPALTAPVDPVTALRRIAFLMERQRAGSYRIEAFRKAAAT
                     ISALPDGELAARHTAGTVQDLPGIGKATAGVIGEALEGELPAYLRSLQDNNTSLLKPG
                     GEALYAALAGDAHTHSNWSDGGSPIEEMVLTAVETGHEWMVLTDHSPQLKVARGLTVE
                     RLTKQIAIVDAITASLAGTFTLLKGIEVDILDDGSLDQTPAMLAKLDLVVASVHSKLR
                     MNAEPMTRRMVAAVRNPRVNILGHCTGRLVTGSRGTRPQSDFDARAVFEACAEHDVAV
                     EINSRPERCDPPDELILLAQEIGCLFSIDSDAHAPGQLDMKAYGAERATRLGIDPDRI
                     VTTWDVDRLREWAGRRA"
     gene            complement(1394..2227)
                     /locus_tag="PKU75_15920"
     CDS             complement(1394..2227)
                     /locus_tag="PKU75_15920"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPF82413.1"
                     /translation="MAMSRNPVLITGCSTGIGRATADLLLKAGYTVYATARRPETLAE
                     LAVAGAQTLALDVTSEESMAAAVGEVERAHGAVGTLINNAGYGEYGPIEEADLDAVRR
                     MFETNVFGLARMIQLVLPGMRTAGGGLIVNIGSMGGRIAFPIGGYYHATKYAVEGITD
                     ALRNEVREFGIDVTLIEPGPIRTQFEETINSSAAVANSDSPYADLVAKVRSVNSGAYA
                     NKLLSVGPDAVAKAILKAVESDPPKSRYLVTAAARAMVHTRRIGGDLVWDAVVNRQFK
                     H"
     gene            complement(2254..3462)
                     /locus_tag="PKU75_15925"
     CDS             complement(2254..3462)
                     /locus_tag="PKU75_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HPF82414.1"
                     /translation="MTYDVAAVRAQFPALEEGAAHFDGPGGTQSPRQVGEAVSRTLTS
                     AVANRGFVTRAERRAEEAVTQCRSALADLLGADPRGVVFGRSMTDLTYDFSRTLAKTW
                     HPGDEVVVTRLDHDANVRPWVQAAEAVGALPRFADFDPTTGELGPDDIAAHLSPTTRL
                     VAVTAASNLIGTMPDIPAIARLVHERSDALVYVDGVHYTAHESVDLAASGADFWACSP
                     YKFLGPHCGVVAADPALLETLRPDKLLPATDEVPERFELGTLPYELMAGTTAAIDFLA
                     GLAEGHAGSRRDRLVAGYAAMHQHEKHLRDLLEPTLRDLPGVQVYSNAGRRTPTILFT
                     IDGWDNQQLRTEMAARWINAPAGNFYAIECSRHLGLGDAGGIRVGLAPYTSEDDIERL
                     LAALREMLLG"
BASE COUNT          537 a         1240 c         1324 g          567 t
ORIGIN      
        1 cgcgacctcc gccgtgacag ggcggcgcgc taaccaactg cgctacgggg ccttgtgtaa
       61 ggcctttact gcgtatcccc aacgggattc gaacccgtgt tgccgccgtg aaagggcgga
      121 gtcctgggcc gctagacgat ggggacccga tctcgccggg accccgatcg catgagcgag
      181 cagggctcga atccgtcgag gactccgaaa tcataagggt cgccgccgca atgtccaaaa
      241 cgaggggtct cccaacgtcg agtgagcgcg acacgcccat gtttcgctcg ggaaccgggc
      301 gtgtcgcgct cactcgactg ggatctggtg tcacgcccgg cgcccggccc actcccggag
      361 ccggtcgacg tcccaggtcg tgacgatccg gtcggggtcg atgcccaggc gggtggcccg
      421 ctcggcgccg taggccttca tgtccagctg cccgggcgcg tgggcgtcgg aatcgatgct
      481 gaacaggcag ccgatctcct gcgccagcag gatcagctcg tccggcgggt cgcaccgctc
      541 gggccgggag ttgatctcga cggcgacgtc gtgctcggcg cacgcctcga agacggcgcg
      601 ggcgtcgaaa tcgctctgcg ggcgggtgcc gcgagacccg gtgaccagcc gcccggtgca
      661 atgcccgagg atgttgaccc gagggttgcg caccgccgcg accatccgcc gcgtcatcgg
      721 ctcggcgttc atccgcagct tcgaatgcac gctcgcgaca acgagatcca gcttggcgag
      781 catggctggg gtctggtcga ggctgccgtc gtcgaggatg tcgacctcga tgcccttgag
      841 cagggtgaag gtgcccgcga gcgaggcggt gatggcgtcg acaatggcga tctgcttggt
      901 caggcgctcg acggtcagcc cgcgggcgac cttcagctgg ggggagtggt cggtcaggac
      961 catccactcg tggccggtct cgaccgcggt gagcaccatc tcctcgatgg ggctgccgcc
     1021 gtcggaccag ttggagtgcg tgtgcgcgtc gccggccagc gcggcataca gtgcctcacc
     1081 gccgggtttc agtaggctgg tgttgttgtc ctgcaacgat ctcaggtatg ccgggagctc
     1141 gccctccagg gcctcgccga tgactcccgc ggtcgccttc ccgatgccgg gcaggtcctg
     1201 gacggtgccg gcggtatgcc gcgcggccag ctccccgtcg ggcagcgcgc tgatcgtcgc
     1261 ggcggccttg cggaaggcct cgatgcgata ggagccggcc cgctggcgct ccatcaggaa
     1321 ggcgatgcgc cgcagcgcgg tgaccggatc gacgggcgcc gtcagcgccg ggacgagggc
     1381 gaccatccgc tgatcagtgc ttgaactgcc ggttgaccac ggcgtcccag accaggtcgc
     1441 ccccgatccg gcgcgtgtgc accatggctc gggcagcggc cgtgacgaga tagcgcgact
     1501 tcggcgggtc ggactcgacg gccttgagga tcgctttggc gaccgcgtcc gggccgacgg
     1561 acagcaactt gttcgcatag gcgccggagt tgaccgaccg caccttggcg accagatcgg
     1621 catacggcga atccgaattg gccacggccg cagaggaatt gatcgtctcc tcgaactggg
     1681 tgcggatggg cccgggctcg atcagggtga cgtcgatgcc gaactcgcgc acctcgttgc
     1741 gcaaggcatc cgtgatgccc tccaccgcat acttcgtcgc gtggtagtag ccaccgatcg
     1801 ggaaggcgat ccgcccgccc atcgacccga tgttgacgat caggccgccg ccggccgtgc
     1861 gcattcccgg caggacgagc tggatcatcc gggccagccc gaagacgttc gtctcgaaca
     1921 tccggcgcac cgcgtcgagg tccgcctcct cgatcgggcc gtactcgcca tacccggcgt
     1981 tgttgatcag cgtcccgacg gccccgtgcg cgcgctcgac ctcgccgacg gccgccgcca
     2041 tcgactcctc gctcgtgaca tcgagggcca gcgtctgggc accggcgacg gcgagttcgg
     2101 cgagggtctc ggggcggcgg gcggtggcat agaccgtgta gccggccttg agcagcaggt
     2161 cggcggtggc gcgcccgatc cccgtcgagc agccggtgat gaggacggga ttgcgggaca
     2221 tcgccatggg gcaactctag gttgcgcgtc cagtcagccc agcaacatct cgcggagcgc
     2281 ggcgagaagt ctctcgatgt cgtcctcgct cgtgtagggc gccaggccga cccgtatccc
     2341 gccggcgtcg ccgaggccga ggtggcgcga gcactcgatc gcatagaagt tgccggcggg
     2401 agcgttgatc cagcgggccg ccatctcggt gcggagttgc tggttgtccc agccgtcgat
     2461 cgtgaacagg atcgtcgggg tgcggcggcc ggcgttcgaa tacacctgca ccccaggcag
     2521 atcgcgcagc gtgggttcga gcagatcccg caggtgcttc tcgtgctggt gcatcgcggc
     2581 atacccggcc accagccggt cgcggcggga tccggcgtgg ccttcggcaa gcccggcgag
     2641 gaagtcgatg gcggcggtcg tgcccgccat gagctcgtat ggcagtgtcc ccagctcgaa
     2701 ccgctccggc acctcgtccg tggcgggcag cagcttgtcc gggcgcaggg tctcgagcag
     2761 ggccgggtcg gcggcgacga cgccgcagtg cgggccgagg aatttgtagg gcgagcaggc
     2821 ccagaagtcg gccccgctcg ccgccaggtc gacgctctcg tgggccgtgt agtggacccc
     2881 gtcgacatag acgagtgcgt cgctgcgctc gtgcaccagc cgggcgatgg ccgggatgtc
     2941 gggcatggtg ccgatgagat tggaagccgc ggtcacggcg acgaggcgcg tcgtggggga
     3001 gaggtgcgcg gcgatgtcgt ccgggcccag ctcgccggtc gtcgggtcga agtcggcgaa
     3061 ccggggcagc gcaccgaccg cctcggcggc ctgcacccac ggccggacgt tggcgtcgtg
     3121 gtcgagccgg gtgacgacga cctcgtcgcc ggggtgccac gtcttggcca aggtgcggga
     3181 gaagtcgtag gtcaggtccg tcatcgaccg gccgaagacg acgccgcgcg gatcggcgcc
     3241 caggagatcg gcgagggccg aacggcactg tgtcacagcc tcttccgcgc gccgctcggc
     3301 ccgggtgacg aacccgcggt tggccaccgc gctcgtcagc gtccggctga ccgcctcacc
     3361 gacctgccgc ggcgactggg tgccgcccgg cccgtcgaag tgcgccgccc cctcctccag
     3421 agcagggaac tgcgcacgca cggctgccac gtcatacgtc atgacccgaa tctagcgagc
     3481 gggctcctgc gtaggctgac cgagtgaatg ctccgacccc gctcttcacc ctgggcgacc
     3541 tcgccgccgc acacgacctg gtggcccgac atgtgccgcg gacgccggca tacgcctggc
     3601 cgttgctggc gcgtgcagtt ggtgcggagg cagtggtcga gcacgagaac cagacgccga
     3661 ccggcgcc
//
LOCUS       DAOPJG010000260         3665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_28016_length_3665_cov_5.382548, whole genome shotgun sequence.
ACCESSION   DAOPJG010000260 DAOPJG010000000
VERSION     DAOPJG010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28016_length_3665_cov_5.382548"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..722)
                     /locus_tag="PKU75_15930"
     CDS             complement(126..722)
                     /locus_tag="PKU75_15930"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPF82415.1"
                     /translation="MSDALIVGADGLARPSWAAGDPLLRDYYDTEWGMPVRDERGVFE
                     RLSLEAFQSGLSWATILRKRPAFREAFDGFDPDLVAAYGDRDRQRLMADAGIVRNRRK
                     IDATITNARATIALREQGGLADFVWSFQPADTPTPTAYDQIPTQSAESLAMSKALKKA
                     GFAHVGPTTMFALMEAIGIVDTHLLGSHRRASSGVWPA"
     gene            complement(749..1258)
                     /locus_tag="PKU75_15935"
     CDS             complement(749..1258)
                     /locus_tag="PKU75_15935"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPF82416.1"
                     /translation="MTATVHTVIDSPVGPLTLTAEGGALTGVYFEVHRRRHKLPDLGQ
                     AIVGEPVLVEAERQLGEYFAGQRREFDLPLAPRGEAFQHAVWAQLREIPYGATRTYGE
                     IARAIGQPGAAQAVGVANGANPISIIVPCHRVIGADGSLTGYAGGLDRKRLLLELEQP
                     PADEAGRLF"
     gene            1356..1670
                     /locus_tag="PKU75_15940"
     CDS             1356..1670
                     /locus_tag="PKU75_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82417.1"
                     /translation="MGLFRRRPRSSAGEPISDSKAARTHLQQFASTRRGVEAYVEPPT
                     NVTAPTIVLVAWDGEWTRRAVRTRDEAFELGNSLGVPTYDVQATGYPSRMREWTSRQR
                     KR"
     gene            complement(1794..2114)
                     /locus_tag="PKU75_15945"
     CDS             complement(1794..2114)
                     /locus_tag="PKU75_15945"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HPF82418.1"
                     /translation="MNESKKGRVTEQIEVAGDQLVGKVKELYADGKTRRIVLHSEDGK
                     ELFSLPMNVGVIGGGVLALASPVIAAVGAVAALATKVRIDVVREGDDDDPQDAPAEDP
                     TNPS"
     gene            complement(2219..2935)
                     /locus_tag="PKU75_15950"
     CDS             complement(2219..2935)
                     /locus_tag="PKU75_15950"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HPF82419.1"
                     /translation="MTGTVAPVLADALRARGDEIVTWDRERVRVDHAPAVRAFVDATA
                     PDGICHLAMGSPDWAAAMAGLCGQRGIPFLFTSSVSVFGPEQRGPLGIEITPTATDDY
                     GRYKRDCEVAVAGANPAALIPLLGWQIGHAPGSNNMVDHLVRAAGGATVRASTDWFPS
                     CAFLDDTAAALVDLLDRGDPGLFHLEGNPGLSYFEIVTQLARTHRLEAHVVATDDVVM
                     DNRMEDPRIHVRPITERLVS"
     gene            3062..>3665
                     /locus_tag="PKU75_15955"
     CDS             3062..>3665
                     /locus_tag="PKU75_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPF82420.1"
                     /translation="MARALDGAAGLEAAAVALAGELIGIDSVNPGLVPGAAGEADIVA
                     HLGERLSRNGFQTEIVEAAVAERPSLVAWPRSGTGAMPMLNGHVDTVGVDGMPDAFTP
                     RVEGDRLLGRGACDMLGGVAGMVVAAEYAVTHGVPAALALVSDEEDRSLGSEAVIAAL
                     PSLGLRPTLCLVGEPTWLAPAASLRGYAVADVTFTGRASHT"
BASE COUNT          531 a         1286 c         1277 g          571 t
ORIGIN      
        1 gcacccgctg atcgactcgg tctcggcggc gcgggagctc ttcgcggact atcgccatgc
       61 ccaccgggag ttgggtgccc tgttctcctg aacgcccgtc atcccgtgga gagcgccgga
      121 agatctcagg ccggccagac gccggagctg gcgcggcggt gggagccgag gaggtgggtg
      181 tcgacgatgc cgatggcctc catgagggcg aacatcgtcg tggggccgac gtgggcgaag
      241 ccggccttct tcagggcctt ggacattgcc agggactccg ccgactgcgt cgggatctgg
      301 tcgtacgccg tgggcgtggg tgtgtcggcc ggctgaaagg accagacgaa gtcggcgagg
      361 ccgccctgct cgcgcagggc gatcgtcgcg cgagcgttcg tgatggtcgc gtcgatcttg
      421 cgccggttgc gcacgatgcc ggcgtcggcc atcagccgct gccggtcccg atcgccgtat
      481 gccgcaacga gatccgggtc gaagccgtcg aacgcctcgc ggaaggcggg gcgcttgcgc
      541 aggatcgtcg cccacgacaa accggactgg aaggcctcca ggctgaggcg ctcgaacacg
      601 ccgcgctcgt cgcgcaccgg cataccccat tcggtgtcgt agtagtcgcg caacagcgga
      661 tcgcccgccg cccacgacgg gcgcgcgagg ccgtccgcac cgacgatcaa tgcatcgctc
      721 acagtcgcac cctagagggc acggtgcctc agaacaggcg gccggcctcg tcggcgggcg
      781 gctgctcgag ttcgagcagc aggcgcttgc ggtccaggcc gccggcatac ccggtcaagg
      841 acccgtcggc gccgatgacc cgatggcacg ggacgatgat gctgatcggg ttggcgccat
      901 tggccacgcc caccgcttgg gcggcgcccg gctgcccgat cgcccgggcg atctcgccgt
      961 aggtgcgggt cgcgccatac gggatctcgc gcagctgcgc ccacacggcg tgctggaagg
     1021 cctcgccacg cggtgccagc ggcaggtcga actcgcgccg ctgccccgcg aagtactccc
     1081 ccagctgccg ctccgcctcg acgagcaccg gctcgccgac gatggcctga cccaggtccg
     1141 gcagcttgtg ccggcggcgg tggacctcga agtacacgcc cgtgagggcg ccaccctcgg
     1201 cggtcagggt gagcggcccg acgggactgt cgatcacggt gtggacggtg gcggtcatga
     1261 gaactgtcat accccatcag tcgcggcagc tctgtcgaat gtgaggcccg gcgcgcattc
     1321 agcaactccc cagccaatct ccataccatc tgctcatggg gctattccgg cgacggccgc
     1381 gcagcagcgc tggagagccc atttccgatt cgaaggcagc gcgcacgcat ctgcagcagt
     1441 tcgcctcgac ccgccgcggc gtcgaggcct atgtcgagcc gccgaccaat gtgaccgctc
     1501 cgacgatcgt gctcgtcgcg tgggacggcg aatggacccg ccgcgcggtg cgcacccggg
     1561 acgaggcatt cgaactcggc aactccctgg gcgtgccgac gtatgacgtg caggcgacgg
     1621 gctatccgtc acgaatgcgg gagtggacct ctcgccagcg caagcgctga cggctgcgaa
     1681 ttccgttcgc ggacgcgttc gccccgcctt cgtcgtcggg aacccggtag gacgaagcga
     1741 tgggcgcccg cgatccggtc gttcggatcg gggcgcccat cggcgaggaa ggctcaggac
     1801 gggttcgtcg ggtcctcggc cggcgcgtcc tggggatcgt cgtcgtcgcc ctcacggacg
     1861 acgtcgatgc ggaccttggt ggccagcgcg gcgacagcgc cgaccgcggc gatcaccgga
     1921 gaggcgaggg caaggacgcc cccgccgatg acgccgacat tcatgggcag gctgaacagc
     1981 tccttaccgt cctcgctgtg cagcacgatg cgacgcgtct tgccatctgc gtacagttcc
     2041 ttgaccttgc ccaccaactg atcgccggcg acctcgatct gctcggtgac cctgcccttt
     2101 ttcgactcgt tcattggctt cgcgccctcc tgcggtgcat gcgttcggat ctgacgttct
     2161 cagcctaggg cgcacgtgca cgctccggcc gtggggcgcc tccctgatcc cgcccgggtc
     2221 aggacaccag ccgctcggtg atcggccgga cgtggatccg cgggtcctcc atccggttgt
     2281 ccatcaccac atcgtccgtc gccacgacat gcgcctccaa ccggtgtgtg cgtgcgagtt
     2341 gggtgacgat ctcgaagtac gacaggccgg ggttgccctc gagatggaag agccccgggt
     2401 cgccacgatc cagcaggtcg acgagtgccg ctgccgtgtc gtccaggaag gcgcaggacg
     2461 ggaaccagtc ggtgctcgct cggaccgtcg ccccgccggc ggcccgcacc aggtggtcga
     2521 ccatattgtt cgacccgggg gcatggccga tctgccaccc gagcaggggg atcagtgccg
     2581 cgggattcgc ccctgcgacc gcgacctcgc agtcacgctt gtagcggccg tagtcgtccg
     2641 tggcggtcgg cgtgatctcg atgcccaacg ggccgcgctg ctccggcccg aacaccgaca
     2701 ccgagctggt gaacaggaac gggatgccgc gctgcccgca caggccggcc atcgcagccg
     2761 cccagtcagg gctgcccatc gcgaggtggc agatcccatc gggcgccgtg gcgtcgacga
     2821 aagcccggac ggccggcgcg tgatcgaccc gcacccgttc gcgatcccac gtcacgatct
     2881 catctcctcg cgctctcagc gcgtccgcga ggacaggtgc gacggtgcca gtcatcccag
     2941 tcacgatcca ccgcatccga atgctccttc gtccgttcgc gggcgggagc accgctcgta
     3001 tgctgatccg gctcacccct catcgagagt gcccgacgag ccgaggtgaa atcatgactg
     3061 catggcgcgc gctctcgacg gggctgccgg gctcgaggcg gcggcggtcg ccctcgcggg
     3121 agagctcatc ggcatcgact cggtcaaccc cggcctcgtc cccggtgcgg cgggcgaggc
     3181 ggacattgtg gctcacctgg gggaacgtct gtctcgcaat ggtttccaga ccgagatcgt
     3241 tgaggcagcg gtggcggagc gaccgagcct ggtggcgtgg ccgaggtccg gcactggggc
     3301 gatgccgatg ctcaacgggc acgtcgacac cgtcggtgtc gacgggatgc ccgacgcctt
     3361 cacgccgcgc gtcgaaggtg accgcctcct cggccgcggg gcgtgcgaca tgctcggcgg
     3421 ggtcgcgggc atggtggtcg ccgcggagta tgccgtgacg cacggcgtcc ccgctgccct
     3481 cgccctggtc tcggacgagg aggaccgtag cctgggcagc gaggccgtca tcgccgcact
     3541 cccatccctg gggctgcggc cgacgctgtg cctcgtgggt gagccgacct ggctcgcccc
     3601 cgccgcgtcg ctgcgcgggt atgccgtcgc cgacgtcacc ttcaccggcc gggcgagtca
     3661 cacct
//
LOCUS       DAOPJG010000261         3660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_28077_length_3660_cov_6.157559, whole genome shotgun sequence.
ACCESSION   DAOPJG010000261 DAOPJG010000000
VERSION     DAOPJG010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28077_length_3660_cov_6.157559"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /gene="uvrA"
                     /locus_tag="PKU75_15960"
     CDS             complement(<1..1770)
                     /gene="uvrA"
                     /locus_tag="PKU75_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPF82421.1"
                     /translation="MGGLSEVPPSIAVVTISTSKRGAGSRLHDKLIVRGAREHNLKDV
                     SVELPRDSLVVFTGLSGSGKSSLAFDTIFAEGQRRYVESLSAYARQFLGQMDKPDVDF
                     IEGLSPAVSIDQKSTNRNPRSTVGTITEVHDYLRLLYARAGRPHCPVCMEPITKQTPQ
                     QIVDRLMELPDGTRFQVLAPVVRARKGEYVELFHELQSKGFARARVDGQVVTLSDHPK
                     LEKQVKHTIDVVVDRLVAKNSDSEAGRSAKRRLTDSVETALSLAGGLLVVDFVDLPAD
                     DPGRERRFSERMACPNEHPLAMDEIEPRSFSFNSPFGACPKCTGIGVELEVDPELIVP
                     DEDLSLAQGAIAPWGQGSGSAEYFQRVLTALAEDLTFSMDTPWKALPERAKTAVLHGK
                     NHQVHVRYRNRYGRERSYSTGFEGAVPFVKRRHAETESDWSRERYEGYMREVPCPACA
                     GTRLKPESLAVLIGGQSIAAVSRLSIKEAAGFLEAVDFTTRERAIAERVIKEINARLG
                     FLLDVGLDYLSLDRPAGTLSGGEAQRIRLATQIGSGLVGVLYVLDEPSIGLHQRDNHR
                     LIETLERLRDLGNTLIVVEHDEDT"
     gene            1841..2557
                     /locus_tag="PKU75_15965"
     CDS             1841..2557
                     /locus_tag="PKU75_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPF82422.1"
                     /translation="MNAAASEPTRGSALADAAGGVLDQVKPKLRGWLHLGAFPLAVVA
                     GIVLVGLAPPGRGRIACAVFALSAAALFGTSALYHRGHWSPRVARLLKRIDHSNIFVI
                     IAGSYTPFTVLLLPPDQARTLLWIVWSGALLGVAFRVFWVGAPRWLYTPAYVALGWTA
                     VFYLPDFLQRGGVAVLTLVLVGGILYTLGGLVYGIKRPNPIPRWFGFHEVFHAFTIAA
                     FVVHMVGVFLAVYTPVAQSG"
     gene            complement(2566..3270)
                     /locus_tag="PKU75_15970"
     CDS             complement(2566..3270)
                     /locus_tag="PKU75_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF82423.1"
                     /translation="MLEVKDLEVSYGKIKAVKGISFTVGQGEVVSLIGTNGAGKTTTL
                     RTISGLLRPSAGSITFQGKRIDDVPAHEIVVLGIAHCPEGRRIFPQMTVEENLLLGAF
                     TREDGEIGKDLQEAYELFPILQERSKQPAGTFSGGEQQMLAMGRAMMSRPKLLMLDEP
                     SMGLSPLMMKRIMTTVRTLQDRGTTILLVEQNAQAALRLANKGYVLEVGKVVLQGTGR
                     ELLTSDDVRKAYLGED"
     gene            complement(3321..>3660)
                     /locus_tag="PKU75_15975"
     CDS             complement(3321..>3660)
                     /locus_tag="PKU75_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPF82424.1"
                     /translation="MPYGDQRRLEIARALATDPKLILLDEPTAGMNPLETQQAMDLIF
                     KIRDQGLAVVVIEHDMRFIFQLCDRVLCLVRGEALVWGTPGEVQSDPRVVEAYIGGSE
                     LEDDEDEVQA"
BASE COUNT          542 a         1251 c         1212 g          655 t
ORIGIN      
        1 ggtgtcctcg tcgtgctcga cgacgatgag ggtgttgccg aggtcgcgca gccgctccag
       61 tgtctcgatg agccggtggt tgtcgcgctg atgcaggccg atcgacggct cgtcgaggac
      121 gtagagcacc cccaccaacc cggagccgat ctgggtcgcg agccggatcc gctgcgcctc
      181 gccgccggac aacgtccccg ccggccggtc cagcgacagg tagtccagtc ccacatcgag
      241 caggaagccc agccgggcgt tgatctcctt gatcacccgc tcggcgatgg cgcgctcgcg
      301 cgtcgtgaag tcgaccgctt cgaggaaacc ggccgcctcc ttgatcgaca gcctcgacac
      361 ggcggcgatc gactgcccgc cgatgagcac cgccagggac tcgggcttca ggcgcgtgcc
      421 cgcacaggcg gggcagggca cctcacgcat gtagccctca taccgctccc ggctccagtc
      481 ggactcggtc tcggcatgac ggcgtttgac gaacggcacc gccccctcga agccggtgct
      541 gtaggagcgc tcacggccgt aacggttgcg gtagcgcaca tggacctgat ggttcttgcc
      601 gtgcaggaca gcggttttcg cgcgctctgg cagtgccttc cacggcgtgt ccatcgagaa
      661 cgtcaggtcc tcggcgagcg ccgtcaagac ccgctggaag tattcggccg agccgctgcc
      721 ctggccccat ggcgcaatgg ctccctgcgc cagggacaag tcctcgtccg ggacgatgag
      781 ttccggatcg acttcgagtt cgacgccgat acccgtgcac ttggggcacg ccccgaaggg
      841 gctgttgaag gagaaggagc gcggctcgat ctcgtccatc gccagcgggt gctcgttggg
      901 gcacgccatc cgctccgaga agcgccgctc ccgcccgggg tcgtcggccg gcaggtcgac
      961 gaagtccacc acgagcagcc caccggccag cgagagcgcc gtctcgaccg agtcggtgag
     1021 ccggcgcttg gccgagcgcc ccgcctcact gtcggagttc ttggccacca accggtcgac
     1081 gacgacgtcg atggtgtgtt tgacctgctt ctccaacttg gggtgatcgg agagggtgac
     1141 cacctgcccg tccacccgcg cccgcgcgaa acccttgctc tgaagctcgt ggaacaactc
     1201 gacatactcg cccttgcgag cccgcaccac cggcgcgagc acctggaagc gcgtgccgtc
     1261 gggcaactcc atcagccggt cgacgatctg ctgcggagtc tgtttggtga tcggctccat
     1321 gcacacgggg cagtgggggc gaccggctcg ggcatacagc agacgcaggt agtcgtggac
     1381 ctcggtgatc gtgcccaccg tcgagcgcgg gttgcggttg gtcgacttct ggtcgatgga
     1441 gacggcgggg gagagccctt cgatgaagtc gacatcgggc ttgtccatct gcccgaggaa
     1501 ctggcgcgca taggccgaca gcgactcgac atagcgccgc tggccctcgg cgaaaatagt
     1561 gtcgaacgcg agggaggact tgcccgaccc ggacagcccg gtgaagacga caaggctgtc
     1621 tcggggcagt tccaccgaga cgtccttgag gttgtgctcc cgtgcgccac gcacgatgag
     1681 cttgtcgtgg agccggcttc cggcgccacg tttcgaggtc gagatcgtca cgacggcaat
     1741 gctaggcggc acctccgaca atccccccat ccgaactccc caaggctcga ttcggaacta
     1801 ccggcaagta acctacggtt gcgtaggtta cgctggaggg gtgaacgcgg cagcgtctga
     1861 accaacgcgt ggctccgccc tggccgatgc ggccggcggg gtgctcgatc aggtcaagcc
     1921 gaagttgcgg ggctggttgc atctgggcgc cttcccgctc gccgtggtgg ccgggatcgt
     1981 cctcgtcggc ctggccccac ccgggcgagg tcgcatcgcc tgcgccgtgt tcgccctcag
     2041 cgctgcggcc ctgttcggca cgagcgcgct ctatcaccgg ggccactggt cccctcgcgt
     2101 cgcgcgcctg ctcaagcgca tcgaccactc caatatcttc gtcatcatcg cgggcagtta
     2161 cacgccgttc accgtgctct tgctcccgcc ggaccaggcc cgcaccctgt tgtggatcgt
     2221 atggagtggc gcgctgctcg gggtcgcctt ccgggtgttc tgggtcggcg ccccccgctg
     2281 gctctacacg cccgcgtatg tcgcgctggg ctggaccgcc gtcttctacc tgcccgactt
     2341 cctgcagcgc ggcggcgtgg ccgtcctgac gctcgtgctc gtcggtggca tcctctatac
     2401 cctcggcggc ctcgtttacg gcatcaagcg tcccaacccg atcccgcggt ggttcggctt
     2461 ccacgaggtg ttccacgcct tcaccatcgc ggccttcgtc gtgcacatgg tcggggtctt
     2521 cctcgccgtg tacacccctg tcgcccagtc cggctagtcg ggcggtcagt cttctcccag
     2581 gtaggccttg cggacgtcgt cggaggtgag cagctcccgg ccggtgcctt ggagcacgac
     2641 cttgccgacc tcgaggacgt agcccttgtt ggccaagcgc agggcggcct gcgcgttctg
     2701 ctcgacgagc aggatggtgg tgccgcggtc ctgcagcgtg cggacggtcg tcatgatgcg
     2761 cttcatcatc agcggcgaca ggcccatcga cggctcgtcc agcatcagca gcttgggtcg
     2821 cgacatcatc gcgcgtccca tggcgagcat ctgctgctca ccgccggaga acgtgccggc
     2881 cggctgcttg gagcgttcct gcaggatcgg gaacagctcg taggcctcct gcaggtcctt
     2941 gccgatctcg ccgtcttcgc gagtgaaggc gccgagaagg aggttctcct cgacggtcat
     3001 ctgcgggaag atgcgccggc cctcggggca gtgcgcgatg ccgagcacca cgatctcgtg
     3061 cgcgggcacg tcgtcgatcc gcttgccctg gaaggtgatc gagcccgcac tcgggcgcag
     3121 caggcccgag atcgtccgca gggtcgtggt cttgccggcg ccattggtgc cgatgagcga
     3181 gacgacctcg ccctggccga ccgtgaacga gatccccttg acggccttga tcttgccgta
     3241 gctgacctcg aggtccttga cctccagcat tgccggggcc gatgcgggtc gggtcaggtc
     3301 ggattcgtga accgcgtgac tcatgcctgc acctcgtcct cgtcgtcctc gagttcgctg
     3361 ccgccgatat aggcctcgac gacgcgcgga tcggattgca cttcaccggg tgtgccccac
     3421 acgagggcct cgccgcgcac gagacacagc acccggtcgc agagctggaa gatgaaccgc
     3481 atgtcgtgct cgatgacgac gacggccagg ccttggtcac ggatcttgaa gatcaggtcc
     3541 atggcctgct gcgtctccag cgggttcatg ccggccgtgg gttcgtcgag caggatcagc
     3601 ttggggtccg tcgccagcgc ccgggcgatc tccaggcgcc gctggtcgcc atacggcata
//
LOCUS       DAOPJG010000262         3639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_28293_length_3639_cov_5.152344, whole genome shotgun sequence.
ACCESSION   DAOPJG010000262 DAOPJG010000000
VERSION     DAOPJG010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28293_length_3639_cov_5.152344"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PKU75_15980"
     CDS             <1..802
                     /locus_tag="PKU75_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPF82425.1"
                     /translation="YAGLGIADAQGTIRVVDKLDKIGPDAVRELLRERGLTGEQADAC
                     LRLAQISAADTSFVGQVRALGVEHPLLDEGLTDLAAVIEVGAAHVPGRVVADLRIARG
                     LDYYTGTVYETQIVGREDWGSVAGGGRYDALASDGRTTYPGVGISIGVTRILGLLFGA
                     GELSASRSTPSVVLVAVNDEAERAASTAVATALRARGIPCEVAPTADKFGKQIRYADR
                     RGIPYVWFSRADGDQVKDIRSGEQVPADPAVWQPPAQDLRPQVTRSGD"
     gene            complement(836..1462)
                     /locus_tag="PKU75_15985"
     CDS             complement(836..1462)
                     /locus_tag="PKU75_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82426.1"
                     /translation="MTVLRGVAHARALGLAAVATAVVWIALTLGWETQFAVEVRFPQQ
                     GRGVGAFEIAAVLLSSLLPPLTPSRYGAVEFLAAPGSRALNAAVSVGLTLVPLTSMPV
                     WLLSAEGRDPFEVDYPSGAGYAGTLWLFAALGQLLFYAGGTLVCLLGTPALWYAVVCA
                     QQAFPTAPFLRDLASGLHWQTGPTVTVVAAVIAVAVAWFGHSIPRSAR"
     gene            complement(1459..2775)
                     /locus_tag="PKU75_15990"
     CDS             complement(1459..2775)
                     /locus_tag="PKU75_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82427.1"
                     /translation="MRRPGGLTWAHALGRPLPFWTYPAMAVLTGLAAFARSRSLLSAP
                     YAQWDITSELWHEGLFPVLAVIAGMATLVVTQVFRRNSVLAGAVRASGANRALATFVF
                     WISAWVVLGLLAGLLPAMWKSARTATWGGLDVRDVLVAVCGVIVAVSLGVGLGAVLRH
                     WVAALLTAVGYGTLMICAMSEAGAPLRLLQPSQHPVFPTPEFSLPWGLVLFQMTFGLV
                     ACVTAVAGGAWFLAHRRGSPLTSGVALTALLALAVTAWAWKPMTRVARPPVEASCAAS
                     PQVCVHPAHDKSLPTAVAVTETLRSVGGSRLFGRTYDDALFTSDAARAGALVFRVDVR
                     PTHGMVTKTLEDAIALDLIEGALPLCGRVDGTLPISAQVTNLTAFEIARRSGISALLE
                     VVHEPQVDDPSLRTRMTSMPTDELIRFVDDNLPALRTCSAGAPEAS"
     gene            complement(2772..3467)
                     /locus_tag="PKU75_15995"
     CDS             complement(2772..3467)
                     /locus_tag="PKU75_15995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPF82428.1"
                     /translation="MALHDISFDLGPGVTALLGPNGSGKTTLLKCVLGLLPTEAGTIT
                     RDERGRTDGVESFGYVPQSPELPGFSTVHDAVAYAGWLYGMSPGKLDAAAHGILELLG
                     LTDLARRRIRTLSGGQRQRVAIAAGMIHDPPLLVLDEPSAGLDPGQRLRVREAVARVT
                     QARATLLSTHLLEDVRHVADRILVLVNGKVGFDGTVDDLYRVIGPSHTGDLGADFEAG
                     YDALIRTLGGDSE"
BASE COUNT          625 a         1284 c         1277 g          453 t
ORIGIN      
        1 ctatgccggc ctcggcatcg cggacgcgca gggcaccatc cgcgtcgtcg acaagctcga
       61 caagatcggc cccgacgccg tgcgcgagct gctgcgcgag cggggcctga ccggggagca
      121 ggcggacgcc tgcctgcggc tggcccagat cagcgccgcc gacacctcct tcgtcgggca
      181 ggtccgcgcc ctgggcgtcg agcacccgct gctcgacgag ggcctgacgg acctcgccgc
      241 ggtcatcgag gtgggcgccg cgcacgtccc cggtcgggtc gtcgccgacc tgcgcatcgc
      301 ccgcggcctc gactactaca ccggcaccgt ctacgagacc cagatcgtcg ggcgcgagga
      361 ctggggcagc gtggccggcg gcgggcgcta cgacgcgctg gcctccgacg gccggacgac
      421 ctatccgggc gtcgggatct ccatcggcgt cacccgcatc ctcgggctgc tcttcggcgc
      481 cggcgagctg agcgcctccc gctcgacccc gagcgtggtg ctcgtcgccg tcaacgacga
      541 ggccgagcgc gccgcgtcga ccgcggtcgc cacggcgctg cgggcgcgcg gcatcccgtg
      601 cgaggtggcc cccacggcgg acaagttcgg caagcagatc cggtatgccg accgccgcgg
      661 catcccctac gtctggttct cgcgcgccga cggcgaccag gtcaaggaca tccgctccgg
      721 cgagcaggtg cccgccgatc ctgccgtatg gcagcccccc gcccaggacc tgcgccccca
      781 ggtcacccgg tcgggcgact gacgtcgccc tgcgccgggc ggcccgcccg cccggtcacc
      841 gcgcgctgcg tgggatcgag tgcccgaacc acgcgaccgc gaccgcgatg acggcggcga
      901 cgacggtcac cgtcgggccg gtctgccagt ggagaccgct cgcgaggtcc cgcaggaatg
      961 gggcggtggg gaaggcctgc tgggcgcaga ccaccgcata ccagagggcc ggggtcccga
     1021 gcaggcacac cagggtgccg cccgcgtaga acagcagctg accgagggcg gcgaagagcc
     1081 agagcgtccc ggcgtacccg gcgccggagg ggtagtcgac ctcgaacggg tcgcgacctt
     1141 ccgccgacag cagccagacc ggcatggacg tcagcggaac gagcgtcaga cccaccgaga
     1201 cggccgcatt cagcgcccgc gaccccggcg ccgcgaggaa ctcgaccgcg ccatacctgc
     1261 tcggtgtcag gggcggcagg agggaggaga gcaggacggc cgcgatctcg aatgcgccca
     1321 caccacggcc ctgctgcggg aaccggacct ccaccgcgaa ctgggtctcc cagccgaggg
     1381 taagtgcgat ccacaccacc gcggtggcca cggcggccag cccgagggcg cgcgcatggg
     1441 ccacgccacg cagcaccgtc atgaggcctc cggggcaccc gcggagcacg tccgaagggc
     1501 cggcaggttg tcatccacga agcggatgag ctcgtcggtc ggcatggacg tcatccgcgt
     1561 ccgcagggaa ggatcgtcga cctgcggctc atggacgacc tcgagcaggg ccgagatccc
     1621 ggaccggcgg gcgatctcga aggccgtcag attcgtcacc tgtgcggata tcggcagtgt
     1681 tccgtcgacc cgcccgcaca ggggcaaggc accctcgatc agatcgagcg caatggcatc
     1741 ctcgagggtc ttcgtcacca tcccatgggt cggccggaca tcgacgcgga agaccagcgc
     1801 gcctgcccgg gctgcgtcgg aggtgaagag ggcgtcgtca taggtgcggc cgaagagtcg
     1861 gctcccgccg acagaccgaa gcgtctcggt gaccgcgacg gccgtcggaa gcgacttgtc
     1921 gtgcgccgga tggacgcaca cctgcgggct cgcggcgcat gacgcctcga cgggcggccg
     1981 cgcgacacgc gtcatcggct tccaggccca ggcggtgacg gccaatgcca ggagggcggt
     2041 caaggccacg cctgatgtca gtggagaacc gcgccggtgc gcgagaaacc aggcgccacc
     2101 cgccaccgcc gtgacgcagg cgacgagacc gaaggtcatc tggaacagca caagccccca
     2161 cgggaggctg aactcgggcg tggggaacac cggatgctgt gacggctgca gcaggcgaag
     2221 cggcgccccc gcctcggaca tggcgcagat catcagcgtt ccgtagccca ccgcggtcag
     2281 cagggcggcc acccagtgac gaagaaccgc ccccaggccg actcccagac tcacggcgac
     2341 gatcacgccg cacacggcga cgaggacatc gcgaacatcc aggccacccc acgtcgcggt
     2401 gcgcgcgctc ttccacatgg cgggcaggag accggcgagg agacccagca ccacccaggc
     2461 cgagatccag aagacgaagg tcgccagcgc tcggttagct cccgaggccc ggaccgcacc
     2521 ggccagcacc gagttccgcc ggaagacctg ggtcacgacc aaggtggcca tgcccgcgat
     2581 gacggccagc acgggaaaga gaccctcgtg ccacagctca gaggtgatgt cccactgcgc
     2641 atacggcgcc gagagcaggg agcgcgagcg cgcgaaggcg gccagtccgg tgagcacggc
     2701 catcgcggga taggtccaga acggaagggg gcgccccagc gcgtgcgccc acgtcaggcc
     2761 accgggacgc ctcattcgga gtcgccgccg agggtgcgga tcaaggcgtc atatcccgcc
     2821 tcgaagtctg caccgaggtc tccggtgtgc gacgggccga tcacgcgata gaggtcatcg
     2881 acggtgccgt cgaagccgac cttcccgttg acgaggacaa ggattcggtc ggcgacatgg
     2941 cggacgtcct cgagaagatg cgtcgacagc agcgtcgcac gtgcctgcgt caccctggcc
     3001 accgcttcgc gcacgcgcaa ccgctgacca gggtcgaggc ctgcgctcgg ttcgtcgagc
     3061 acgagcaacg gcggatcgtg aatcatcccg gcagcgatcg cgacccgttg ccgctgacct
     3121 ccggagagtg tgcggatgcg tcgtcgcgcc aggtcggtca gacccaggag ttcgagaatg
     3181 ccgtgtgcgg ccgcatcgag cttccccggt gacattccgt agagccaacc ggcatacgcc
     3241 accgcgtcgt ggacggtcga gaagccaggc aactcgggcg actgagggac atatccgaag
     3301 gactccaccc cgtccgtgcg accccgttca tcgcgggtga tggtgccggc ctcggtcggc
     3361 agtagtccca ggacgcattt caggagagtg gtcttgcccg agccgttggg ccccaagagc
     3421 gcggtcaccc cgggccccag gtcgaaggag atgtcgtgaa gcgccacgtg tcggcggtag
     3481 gcatagcgca aggaggcgac ccgcactatg ggagcttcag gaggcattgt tgctttcagc
     3541 tggccgctgc ggatagcttt tccatccgag gagtttgcgg tgcagtcgga caccatggct
     3601 agatgcccga cagcaccgca cttctgttcg agttatgct
//
LOCUS       DAOPJG010000263         3635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_28344_length_3635_cov_7.765363, whole genome shotgun sequence.
ACCESSION   DAOPJG010000263 DAOPJG010000000
VERSION     DAOPJG010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28344_length_3635_cov_7.765363"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKU75_16000"
     CDS             complement(<1..405)
                     /locus_tag="PKU75_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPF82429.1"
                     /translation="MELTHDFVVPASLDAAWATFMDLEQVGSCFPGATVESVTDEGFT
                     GTVKVKLGPIALVYTGAGKFIERDDAAHRAVIEAKGKDKRGNGTAGATVTVHLTPADG
                     GEHTRVDVTTDLAITGKPAQFGRGVMQDVSDKL"
     gene            complement(410..1258)
                     /locus_tag="PKU75_16005"
     CDS             complement(410..1258)
                     /locus_tag="PKU75_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPF82430.1"
                     /translation="MIPTAFDYVAPESVADALAALADSDDAKVLAGGQSLLPILRMRL
                     NAPDKVVDLGRIAELRGVREDGDAIVIGAMTSYVDLLADPLVAEHAALLKAAIETVAD
                     PQIRRRGTIGGALVHADPAGDVGAPVLALDGELVITGSAGERTVAAADFFEDLFTTAV
                     GEDELLTAVRIPKHTGWGAAYEKFVRVAHQWSIVAVAATVRMEGDTIAEARVGLTNMG
                     STPLRATATEEALAGKQASAEAIAEACAKAADGTNPPSDLNGDGDYRKHLATVFTRRA
                     VLAAAD"
     gene            complement(1255..>3635)
                     /locus_tag="PKU75_16010"
     CDS             complement(1255..>3635)
                     /locus_tag="PKU75_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPF82431.1"
                     /translation="DNIVLPGMLHLAMVRSPFAHAKITSIDTSGAQGLPNVVAVFTAA
                     DFPDGMGACANAWPITPEQVTPDHLPMIGERVACAGEIVAVVVARSAKEARDAAEQVD
                     VDYEELPAVFDAMEAMKDEILAHPDKGTNKSAFWKLDSKEAGTGGDVDEAIAKARQDG
                     VVIEREFRQQRLIPAFMEPRSTVVDPTGEQVTMWSATQIPHILRFLIAATTGMPESKV
                     RVIAPDVGGGFGGKLQTTPEEFITLAVARRLGKPCKYTETRSESLISGHHGRDQYQKL
                     TLAAEKDGTVTGLKVDLVANLGAYVAIVGGGVPVLGAWMFNSIYKFPAYQFNCQTVLT
                     NTTWVDAYRGAGRPEATFGIERIMDELAAELGRDPLEVREQNWIKHEEFPFTSVAGMT
                     YDSGNYEAATARAKELFGYDALRAEQAERRERKDPVQLGIGVSTFTEMCGLAPSRVLG
                     SLAYGAGGWEHASIRMLPTGKVEVITGTSPHGQGHETAWSQIVADRLGVPFEDVEVLH
                     GDTAVAYKGLDTYGSRSLVVGAEAVVRAADKVIEKAKGFAAHMLEASEDDLEFVGGRY
                     GVKGTDKGVGLTDIALATFASHDYPEHLEPGIDADATFDPDNFSFPHGTHLCAMEVDT
                     ETGQSRMRSYVCVDDIGHIINPLIVEGQIHGGLVQGIAQALWEEAIFDENGTLVTGSF
                     VDYTLPTAADTISFVTENTVSPSTTNTLGAKGVGEAGTIASTPAVVNAIVDALRPMGI
                     DDIVMPCTPQRVWKAIQTAGDHSGDVAAQAQPHFDEGAANADPGVTGEGNGQ"
BASE COUNT          513 a         1186 c         1294 g          642 t
ORIGIN      
        1 cagcttgtcc gagacgtcct gcatcacccc acgcccgaac tgggcgggct tgccggtgat
       61 cgccaggtcc gtggtgacgt cgacgcgggt gtgctcgccg ccatcggccg gggtcaggtg
      121 gacggtgacg gtggcaccgg cagtgccgtt gccgcgcttg tccttgccct tggcctcgat
      181 gaccgcgcga tgggcggcgt cgtcacgctc gatgaacttg cccgcgcccg tgtagaccag
      241 ggcgatcggc ccgagcttga ccttgacggt gccggtgaag ccctcgtcgg tgaccgactc
      301 gaccgtggcg ccggggaagc acgaaccgac ctgctccagg tccatgaagg tcgcccaggc
      361 cgcgtcgagc gaggcgggga cgacgaaatc gtgggtgagt tccattggat cagtctgccg
      421 ccgcgagcac cgcccggcgc gtgaagacgg tcgccaaatg tttgcgatag tcgccgtctc
      481 cgttgaggtc gctcggtggg ttggtgccgt cggcggcctt tgcacacgcc tcggcgatgg
      541 cctcggcact cgcctgcttg ccggcgagcg cctcctcggt tgccgtggcg cgcaatggtg
      601 tcgaccccat gttggtcagg ccgacgcggg cttcggcgat ggtgtcgccc tccatccgaa
      661 ccgttgccgc cacggcgacg atcgaccatt gatgggccac gcggacgaac ttctcgtatg
      721 ccgcgcccca ccctgtgtgt ttcgggatgc ggacggcggt gagcaactcg tcctcaccga
      781 cggcggtcgt gaagaggtct tcgaagaagt ccgccgcggc gacggtgcgc tcaccggcgc
      841 tgccggtgat cacgagttcg ccgtcgagag ccaggaccgg ggcgcccacg tctccggccg
      901 ggtcggcatg caccagcgcc ccgccgatgg tgccgcggcg gcggatctgc gggtcggcga
      961 cggtctcgat ggcggccttg agcagggccg cgtgctccgc gacgagtggg tcggcgagca
     1021 gatcgacgta tgacgtcatc gcgccgatga cgatcgcgtc gccgtcctcg cggacgcccc
     1081 gcaactccgc gatgcggccg aggtcgacga ccttgtcggg tgcgttgaga cgcatccgca
     1141 gaatgggcag cagcgactgg ccgccggcca gcaccttggc gtcatccgaa tcggcaagtg
     1201 ccgctaaggc atccgcgacg gactcggggg cgacatagtc gaatgcggta gggatcactg
     1261 gccgttccct tctcccgtga cgccgggatc ggcgttggcc gcgccctcgt cgaagtgcgg
     1321 ttgggcttgc gcggcgacat cgccggagtg gtccccggcg gtctggatcg ccttccatac
     1381 ccgctgcggc gtgcacggca tgacgatgtc gtcgatgccc atcggccgca gcgcgtcgac
     1441 gatcgcgttg accaccgccg gggtggaggc gatggtgccg gcctcgccga ctcccttggc
     1501 gcccaacgta ttcgtcgtcg acggggagac ggtgttctcg gtgacgaacg agatcgtgtc
     1561 ggccgcggtc ggcagcgtgt agtcgacgaa ggagccggtc accagtgtgc cgttctcgtc
     1621 gaagatcgcc tcctcccaca gcgcctgcgc gattccttgg accaggccgc cgtggatctg
     1681 gccctcgacg atgagcgggt tgatgatgtg gccgatgtcg tcgacgcaga catacgaccg
     1741 catccggctc tgcccggtct cggtgtcgac ttccatcgcg cacaggtgcg tgccgtgcgg
     1801 gaaggagaag ttgtcgggat cgaaggtcgc gtcggcgtcg atgccgggct cgaggtgctc
     1861 cgggtagtcg tgcgaggcga aggtcgccag cgcgatatcg gtgaggccga cgcccttgtc
     1921 ggtccccttg acgccgtagc gaccgcccac gaactcgagg tcgtcctcgc tcgcctcgag
     1981 catgtgcgcg gcgaagccct tggccttctc gatcaccttg tccgctgctc gcacaacggc
     2041 ttccgcgccg acgacgagcg aacgcgaacc ataagtgtcg agacccttgt aggcgacggc
     2101 ggtgtcaccg tgcagcacct cgacatcttc gaagggcaca ccgagccggt cggcgacgat
     2161 ctggctccac gccgtttcgt ggccctggcc gtggggcgag gtgccggtga tgacctccac
     2221 cttgccggtg gggagcatgc ggatcgaggc gtgttcccag cctccggcgc cgtatgccag
     2281 tgacccgagg actcgtgagg gcgcgagacc gcacatctcg gtgaaggtcg agacgccgat
     2341 gcccaactgc acggggtcct tgcgctcgcg gcgctcagcc tgctcggccc gcagcgcgtc
     2401 atacccgaaa agctctttgg cccgcgccgt cgcggcctcg taattcccac tgtcgtaagt
     2461 cattccggcg accgaggtga acgggaactc ctcgtgcttg atccagttct gctcgcggac
     2521 ctcgaggggg tcgcggccga gttcggcggc gagttcgtcc atgatccgct cgatgccgaa
     2581 ggtcgcctcc gggcgtccgg ctccgcggta ggcatcgacc caggtggtgt tggtcaggac
     2641 cgtctggcag ttgaactggt aggcggggaa tttgtagatc gagttgaaca tccacgcccc
     2701 gagcaccggc acgccaccgc cgacgatggc gacataggcg ccgaggttgg cgaccaggtc
     2761 gaccttcagg ccggtgaccg tgccgtcctt ttccgctgcg agagtgagct tctggtattg
     2821 gtcgcggccg tggtggccgg agatcaacga ctcgctgcgg gtctcggtgt acttgcacgg
     2881 cttgcctagc cggcgcgcga cggcgagggt gatgaactcc tccggcgtcg tctgcagctt
     2941 gccgccgaag ccgccaccga cgtcgggggc gatcacgcgg accttgctct ccggcatacc
     3001 ggtcgtggcc gcgatgagga agcgcaggat gtgcgggatc tgcgtcgccg accacatcgt
     3061 gacctgctca cccgtgggat cgacgacggt cgagcgcggc tccatgaacg ccggtatgag
     3121 gcgctgctga cggaactccc gctcgatgac gacgccgtcc tgccgggcct tggcgatggc
     3181 ctcgtcgacg tcgccgccgg tgccggcctc cttggagtcc agcttccaga aggcgctctt
     3241 gttggtgccc ttgtcggggt gggcgaggat ctcgtccttc atcgcctcca tggcgtcgaa
     3301 gacggcaggc agttcctcgt agtcgacgtc gacctgctcg gcggcgtcgc gggcttcttt
     3361 ggcggaccgg gcgacgacga ccgcgacgat ctcgccggcg cacgcgaccc gctcgccgat
     3421 catcggcagg tggtcgggag tgacctgctc gggggtgatg ggccaggcgt tggcgcaggc
     3481 gcccatcccg tcggggaagt cggcggcggt gaagacggcc accacattgg gcagaccctg
     3541 ggcgccgctg gtgtcgatgc tggtgatctt ggcgtgggcg aaggggctgc ggaccatggc
     3601 caggtgcagc atgcccggga ggacgatgtt gtcgg
//
LOCUS       DAOPJG010000264         3606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_28688_length_3606_cov_7.691073, whole genome shotgun sequence.
ACCESSION   DAOPJG010000264 DAOPJG010000000
VERSION     DAOPJG010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28688_length_3606_cov_7.691073"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..802
                     /locus_tag="PKU75_16015"
     CDS             212..802
                     /locus_tag="PKU75_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPF82432.1"
                     /translation="MPHRRHLRAPRLGVVLAAGAGVRFGGPKIAAADGAWLAAATQAL
                     REGGCDEVAVCMGAQIVDLPPGIAGIEVPDWTEGMSASVRAAIEHAGRLGATQLLLHL
                     VDLPDVGADVVARVVTAAAGAGPAGLARAAYGGRPGHPVLVGAMHFAPMLATLRGDAG
                     GRGYLADQRGLVVVECGDLASGADVDRRPGVPDSAE"
     gene            799..1227
                     /gene="msrB"
                     /locus_tag="PKU75_16020"
     CDS             799..1227
                     /gene="msrB"
                     /locus_tag="PKU75_16020"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPF82433.1"
                     /translation="MSGLQSDRNYPVTKSDEEWRATLSPQEYAVLRQAGTERPFVGEY
                     TDTKTVGVYACRACGAELFQSETKFESHCGWPSFYAPLAGDSVEYLEDRSLPGRPRVE
                     VRCAACGSHLGHVFEGEGYQTPTDQRYCINSISLTLKPAG"
     gene            complement(1232..1951)
                     /locus_tag="PKU75_16025"
     CDS             complement(1232..1951)
                     /locus_tag="PKU75_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HPF82434.1"
                     /translation="MTERPTPAKPSPARMREINDTIRYAMWSVFAIETRLGEADRAAY
                     AAEIDELIADLAGQDVVVRGLYDVSGLRADADLMIWWHAPDATALQAAYQRFLRTGLG
                     QHLKPVWSQLALHRPAEFNKSHIPAFLADEEARAFVCVYPFVRSYDWYVIDDAERRDM
                     LREHGQMARDYPDVRANTIASFALGDYEWILAFEADELHRIVDLMRELRASRARLHVR
                     EETPFFTGPRVEVATAVAALR"
     gene            complement(1956..3398)
                     /gene="hemG"
                     /locus_tag="PKU75_16030"
     CDS             complement(1956..3398)
                     /gene="hemG"
                     /locus_tag="PKU75_16030"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HPF82435.1"
                     /translation="MYRIVIVGGGIAGLAAAWELVSSAPDVEVTILEQTDRVGGKLRR
                     EEVGGHLVDVGAEAMLALRPEARHLIEEIGAGADLVTPATVAASVYSYGALHPMPPRS
                     MMGIPQQPGDAAGILSDAEVARLAAPEPLPPLESDASVGDYVAAVLGDAVVDRLVEPL
                     LGGVYAGQARKLSLAATMPQLYARAVDGEPPVAYGTQKHEATSPGPKPPPATPDSEKL
                     SAPSPSPFIGLRGGVGRLPEIVADAVRERGVQIRTGVTVRGIAPQPAGGWRLTCGPTT
                     DVEHVYADAVILTTPAAATRKLLEPVAPEAAELIAGIETASAAVVTFAFPSAQMPDLA
                     GSGFLVPPVEGFTVKASTFSSNKWAWMPEQFPDVTYVRASLGRHGDEAALQRSDDDLA
                     DLALTDLTGILGVTLPAPIGVHVQRWGGGLPQYGVGHLDRIALLRKEITNHAGLQVAG
                     AAYDGVGIPAVIASGRRAAQDLAATAGLRD"
BASE COUNT          495 a         1269 c         1337 g          505 t
ORIGIN      
        1 cagcacccgc cgcgcgaaat aggccgccgc gccgagggag gccgcggccg tgccggcggc
       61 cgcggaaccg ccgaccgcgg cggccccgac ggccgccttc ttcgccgcgg tccttctgct
      121 cacccggtca tcctgtcagc ccggaccggt tgtcgcacga ataacgggtg tcactcctgc
      181 aacagccgcc gggtccggcg acaatggcgg tatgccgcat cgccgccacc tgcgcgcccc
      241 gcgcctgggc gtcgtgctcg ccgccggcgc cggcgtccgc ttcggggggc cgaagatcgc
      301 cgcggccgac ggggcctggc tcgccgccgc gacccaagcg ctgcgcgagg gcggctgcga
      361 cgaagtggcc gtatgcatgg gggcccagat cgtcgacctg ccgccgggga tcgccggcat
      421 cgaggtcccc gactggaccg aggggatgag cgcgagcgtg cgcgccgcga tcgagcacgc
      481 cggccggctc ggcgcgacgc agctgctcct ccacctcgtc gaccttcccg acgtcggcgc
      541 ggacgtggtg gcgcgggtgg tgaccgcggc ggctggcgca ggcccggcgg gactggcgcg
      601 ggcggcatac ggcgggcggc cggggcaccc ggtgctcgtc ggcgcgatgc acttcgcccc
      661 gatgctcgcg acgctgcgcg gcgacgcggg cggacgcggc tacctggccg accagcgggg
      721 tctggtcgtc gtcgagtgcg gcgatctggc gagcggcgcg gacgtcgacc gtcggcccgg
      781 cgtgccagac tctgcggaat gagcggtctg cagagcgatc ggaactatcc cgttaccaag
      841 tccgacgagg agtggcgggc gacgctgagc ccgcaggagt atgccgtcct gcgccaggcc
      901 ggcaccgagc ggcccttcgt cggtgaatac accgacacca agaccgtcgg ggtgtatgcc
      961 tgcagggcct gcggcgccga actcttccag tccgagacca agttcgagag ccactgcggc
     1021 tggccgagct tctatgcccc gctggccggt gactcggtcg aatatctcga ggaccgctcc
     1081 ctgcccgggc gtccccgtgt cgaggtgcgc tgtgccgcct gcggcagcca cctcgggcac
     1141 gtcttcgagg gcgagggcta tcagacgccg accgaccagc gctactgcat caactccatc
     1201 agcctgacgc tcaagccggc cgggtaaccc gctatcggag cgccgcgacg gcagtcgcca
     1261 cctcgacgcg cgggccggtg aagaacggcg tctcctcgcg cacgtgcagg cgggcccgcg
     1321 aggcccgcag ttcgcgcatc aggtcgacga tgcggtgcag ttcgtcggcc tcgaacgcga
     1381 ggatccactc gtagtcgccg agggcgaagc tggcgatcgt gttggcgcgc acgtcggggt
     1441 agtcgcgcgc catctggccg tgctcgcgca gcatgtcgcg ccgctcggcg tcgtcgatga
     1501 cgtaccagtc gtaggagcgc acaaacgggt agacgcagac gaaggcccgc gcctcctcgt
     1561 cggcgaggaa ggccgggatg tggctcttgt tgaactccgc cgggcggtgc agcgcgagtt
     1621 ggctccacac cggcttcagg tgctgcccca gcccggtgcg caggaatcgc tggtatgccg
     1681 cctgcaacgc cgtcgcgtcc ggcgcgtgcc accagatcat caggtcggcg tccgcgcgca
     1741 ggccggagac gtcatagagg ccgcggacga cgacatcctg gccggcgaga tcggcgatga
     1801 gctcgtcgat ctccgctgcg tatgccgcgc ggtcggcctc cccgagccgg gtctcgatcg
     1861 cgaagactga ccacatcgcg tagcggatgg tgtcgttgat ctcgcgcatg cgggcggggc
     1921 tcggcttggc cggcgtgggg cgttcggtca tggggtcagt ctctcaagcc ggccgtcgcc
     1981 gccaggtcct gcgctgcgcg gcggccggag gcgatgaccg cggggatgcc gacgccgtcg
     2041 tatgccgcgc ccgccacctg caggcccgcg tggttggtga tctccttgcg cagcaacgcg
     2101 atccggtcca ggtggccgac tccgtattgc ggcagtcccc cgccccagcg ctgcacgtgc
     2161 accccgatcg gtgcgggcag ggtcacaccg aggatgccgg tcaggtcggt cagggcgagg
     2221 tcggccaggt catcgtcgga gcgttgcagg gcggcctcgt cgccgtgccg gcccagggag
     2281 gcccggacat aggtcacgtc ggggaactgc tccggcatcc acgcccactt gttggagctg
     2341 aacgtgctgg ccttgaccgt gaagccttcg accgggggca cgaggaatcc ggagccggcc
     2401 agatcgggca tctgcgcgga ggggaaggcg aaggtgacga cggccgccga ggcggtctcg
     2461 atgccggcga tcagttcggc cgcctccggt gcgaccggtt cgaggagttt gcgggtcgcg
     2521 gcggcgggcg tggtgaggat gacggcgtcg gcgtagacgt gctcgacatc ggtcgtcgga
     2581 ccgcaggtca gccgccagcc gccggccggc tgcggggcga tgccgcggac cgtcacgccg
     2641 gtgcggatct gcacgccgcg ttcgcgcacg gcgtcggcga cgatttcggg cagccggccg
     2701 acgccgccgc gcaggccgat gaagggcgat ggcgatgggg cggagagctt ttcgctgtcc
     2761 ggagtcgccg gcggcggctt cgggcctggg ctcgtggcct cgtgcttctg ggtgccatag
     2821 gcgaccgggg gctccccgtc gaccgcacgg gcatacagct ggggcatcgt cgcggcgagc
     2881 gacagcttgc gggcctgacc ggcatacacc ccaccgagca ggggctccac gagccggtcc
     2941 acgaccgcgt cgccgaggac ggcggcgaca tagtcgccga cggaggcatc cgactccagc
     3001 ggcggcagcg gctcgggcgc cgcgagacgc gcgacctccg cgtcgctgag gatgccggca
     3061 gcgtcgcccg gctgctgcgg gatgcccatc atcgagcgcg gcggcatcgg atgcagcgcg
     3121 ccataggaat agacggacgc ggcgacggtc gccggcgtga cgaggtcggc gcccgcgccg
     3181 atctcctcga tgaggtggcg ggcctcgggg cgcagggcga gcatcgcctc ggccccgacg
     3241 tcgacgaggt gcccgccgac ctcctcgcgg cgcagcttgc cgcccacgcg gtcggtctgt
     3301 tcgaggatcg tcacctcgac gtcgggtgcg ctgctcacga gttcccaggc tgcggccaga
     3361 cccgcgatgc cgcccccgac gataacgatc cggtacatgc ccacaaacat atgtcgccca
     3421 cgcggaacca tcggacagcg ggtagccgtc gcacccccaa agccgtcgac ctgatgggag
     3481 cccctcatga ccaccagcct gatgacctgg gccaatcccg atgtgcgccg ccgcgtgccc
     3541 gctgcactcg ctgcgatcct caccgcgacg gttctgctcg tcgccgggtg cagcggaagc
     3601 gacagc
//
LOCUS       DAOPJG010000265         3517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_29707_length_3517_cov_6.820335, whole genome shotgun sequence.
ACCESSION   DAOPJG010000265 DAOPJG010000000
VERSION     DAOPJG010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29707_length_3517_cov_6.820335"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..981)
                     /locus_tag="PKU75_16035"
     CDS             complement(130..981)
                     /locus_tag="PKU75_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPF82436.1"
                     /translation="MAVVNTSRSAKIGSLLRYSALILAAALFLAPFYLLFRNALMSEA
                     DLGSPRWHWLPPTWEWGNFSAIFNDPALPMTRSLVNSILISIFQTLGVLIISGLAGYG
                     LARIPYRWSNAVFYLITATLLIPAAVTFVPSFVLVSSLGWISTLRGLIIPGLFQALAT
                     FLFRQFFLSFPKEIEEAAFLDGLGYWGTFWRVVVPNSMGFAAAIGTITFIGAWNAFLW
                     PLVIGREQTTWTVQIALSTFLTAQSVNLSQLFAAALVAIAPLLLMFLFLQRWIVEGVE
                     RTGMGGD"
     gene            complement(981..1952)
                     /locus_tag="PKU75_16040"
     CDS             complement(981..1952)
                     /locus_tag="PKU75_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPF82437.1"
                     /translation="MATTPANVDVSAAAAAPTRPPLGARLRGRQDQNLWFWVFVGPFV
                     LGLIIFIYIPILWSIYLSFFESYNTVTPTKFVGIGNYLDMLKDESFRSSLGTFIVFAL
                     FIVPTTFALSLLTAILVARTRVMQAFFRSVFFLPTACSYVVAALIWKMSIFSGVRSGA
                     ANALLGLIGQDSIPWLSVTKPPWYWLVIVSARLWLQVGFYMILFLAALQRISPTLYEA
                     AAIDGASAWQSFRYITLPQLRPTSAAVLLLLLINAFQAFDEFYNILSSFGTYPPYARP
                     PLVYLYYVALGQGQDFGHGSAGAVILTLVILIAALVFNRFFSFRGDD"
     gene            complement(1956..3281)
                     /locus_tag="PKU75_16045"
     CDS             complement(1956..3281)
                     /locus_tag="PKU75_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPF82438.1"
                     /translation="MSDKTIRAGIDRRHLLTLGGGMAVAGALAACGGNTGRGSSSTSA
                     AGSGSGSGSASGGGAKVTLQQWYHQYGEAGTQQAVEGYAKAYTAADVKVTWKPGDYDQ
                     TTAASLLTAGGPDVFEYGNGPTIDMIKGGQVVDLSGLLGDAESDFNPAMVKRMTYDGK
                     LWAIPQVVDMQLLVYRKSLLEKAGVTPPETIDDLMAAAKKLTSGDVKGLFLGNDGGVG
                     LMGAPMLFSAGADYLNEDGSFGFDSDGAAAALGKLRELFTSDSLLLGAPKDWYDASAL
                     TSGLTAMQFTGLWTFPDLTKGLADDYGCIPWPAMPGGAKSVVVGAYGSCVSAKSTNVD
                     AAKEFVKWLWVDQTDKQLDFATAYGFHIPARASLVPQAKTLQSGPAKDAAAYAQEFGH
                     AQSSVLWTPKCATAFGDMMSKIVKDGADVKAEIAKVKAVAEAEVKRVLG"
BASE COUNT          670 a         1105 c         1210 g          532 t
ORIGIN      
        1 ggcgcttcgg gttcctcgac gacatcgttc ccgctgagca cgagaccgcg tgccggcgcg
       61 ccagcgccac catcgcccgc cgcgcggatg tcgagggact cgcctaggcc accccacgaa
      121 gcggcccggt cagtcgccgc ccatgccggt gcgttcgacg ccttcgacga tccagcgctg
      181 caggaagagg aacatcagca gcagtggcgc gatggcgacc agggcggcgg cgaaaagctg
      241 gctgagattg accgattggg cggtgaggaa ggtcgacagg gcgatctgga cggtccaggt
      301 cgtctgctcg cgcccgatga ccagcggcca gaggaaggcg ttccaggcac cgatgaaggt
      361 gatcgtgccg atggccgcgg cgaaccccat cgagttgggc accacgaccc gccagaacgt
      421 cccccaatag ccgaggccgt cgaggaaggc cgcctcctcg atctccttgg gaaaactcag
      481 gaagaactgg cggaagagga aggtcgccag cgcctggaag agtccaggga tgatcaggcc
      541 ccgcagggtc gagatccagc cgagggagga gacgagcacg aagctcggca cgaatgtcac
      601 cgcggctggt atgaggaggg tcgcggtgat cagatagaag acggcgttgg accaccggta
      661 ggggatgcgt gccaggccgt agccagcgag tcccgaaatg atcaacacgc cgagggtctg
      721 gaagatcgag atgaggatcg agttgaccag gctacgcgtc atcggcagag cggggtcgtt
      781 gaagatggcg ctgaagttgc cccactccca cgtcggcggc agccagtgcc acctcggcga
      841 gccgaggtcc gcctccgaca tcaacgcatt gcggaaaagg aggtagaagg gggcgaggaa
      901 gagcgccgcc gccagaatga gcgccgaata gcgaagcagg gagccgattt tggcgctgcg
      961 cgaggtgttg acgacagcca tcagtcgtca cctcggaagc tgaagaacct gttgaacacg
     1021 agcgccgcga tgaggatgac cagggtgagg atgacagcgc cggcgctgcc gtgaccgaag
     1081 tcctgccctt gcccgagcgc gacgtaatag agatagacca ggggcgggcg ggcatacggc
     1141 ggataggtgc cgaacgagct gaggatgttg tagaactcgt cgaacgcctg gaaggcgttg
     1201 atcagtaaca acagcaggac ggccgccgac gtcggccgca gttggggcaa cgtgatgtag
     1261 cggaaggatt gccaggcgct ggcgccatcg atggctgccg cctcatacag cgtgggtgag
     1321 atccgttgca gcgcagcgag aaacaggatc atgtagaacc cgacctgcag ccacagccga
     1381 gcagagacga tcaccagcca gtaccacggc ggcttggtca ccgacagcca cgggatcgag
     1441 tcctgaccga tcaacccgag cagcgcgttg gccgcgcccg agcgcacacc cgagaagatc
     1501 gacatcttcc agatcagggc cgccacgaca tacgagcacg ccgtcggcag gaagaagacg
     1561 gaccggaaga acgcctgcat cacccgggtg cgcgcgacga ggatcgcggt cagcagcgac
     1621 agcgcgaagg tagtcggcac gatgaagagc gcgaagacga tgaaggttcc gaggctggag
     1681 cggaacgact cgtccttcag catgtcgagg tagttgccga tgcctacgaa cttggtcggg
     1741 gtgacggtgt tgtaggactc gaagaagctg aggtagatgc tccagaggat cgggatgtag
     1801 atgaagatga tcagcccgag gacgaagggg ccgacgaaca cccagaacca gaggttctgg
     1861 tcctgccggc cccgcaggcg cgccccgagc ggcggccgcg tgggggcggc cgccgcggcg
     1921 gagacatcga cgttggcggg cgtcgtcgcc atggactagc ccaggacgcg cttgacctcg
     1981 gcctcggcca ccgccttgac cttggcgatc tcggccttga cgtcggcgcc gtccttgacg
     2041 atcttgctca tcatgtcgcc aaaagcggtg gcgcacttgg gagtccagag cacgctgctc
     2101 tgtgcgtggc cgaactcctg agcgtatgcc gcggcgtcct tcgccgggcc ggactgcagg
     2161 gtcttggcct gtggaacgag cgaggcccgc gccgggatgt ggaagccgta tgcggtcgcg
     2221 aagtcgagtt gcttgtcggt ctggtcgacc cacagccact tgacgaactc cttggccgcg
     2281 tcgacattcg tcgacttcgc cgagacgcac gagccatagg cgccgacgac gacggacttg
     2341 gcgccgcccg gcatggccgg ccatgggatg cagccgtagt cgtccgccag ccccttggtc
     2401 aggtcgggga aggtccacaa accagtgaac tgcatggccg tcagtccgct ggtgagcgcc
     2461 gacgcgtcat accagtcctt cggcgcaccc aggagcaggc tgtcgctggt gaacagctcc
     2521 cgtagcttgc ccagggcggc cgccgccccg tcggagtcga acccgaacga accgtcctcg
     2581 ttgagatagt ccgcaccagc ggagaagagc atgggtgcgc ccatgagccc gacgccgccg
     2641 tcgttgccga ggaagaggcc cttgacgtcg ccgctggtga gcttcttcgc ggccgccatg
     2701 aggtcatcga tggtctccgg cggcgtcaca ccggccttct ccagcaacga cttccgatag
     2761 acgagcagct gcatgtcgac gacctgcggg atcgcccaga gcttgccgtc gtaggtcatg
     2821 cgcttgacca tcgcggggtt gaagtcggac tcagcgtcgc cgagcagacc gctcaggtcg
     2881 acgacctgcc cgcccttgat catgtcgatc gtcggaccgt tgccgtattc gaagacgtcc
     2941 ggtccgcccg cggtcagcag cgaggcggcg gtcgtctggt cgtagtcgcc cggcttccag
     3001 gtcaccttga cgtcggcggc cgtgtaggcc ttggcatagc cttcgacggc ctgttgggtg
     3061 ccggcctcgc cgtattggtg ataccactgc tgcagagtga ctttcgcgcc cccgcccgat
     3121 gccgaacccg atccggaccc cgaccctgcg gcgctggtgg acgagctgcc acggccggta
     3181 ttgccgccgc acgcggcgag cgcccctgcc acggccatac cgcccccgag ggtgagcaga
     3241 tgacgccggt cgatcccagc acgaatggtc ttgtcagaca tgggtcctcc gatcgggacg
     3301 ctaccgccgg ttcggcaacg cgttgaggtc aacctagtcc gcccgaccgc atcgatccgg
     3361 gattgccgtg cgccgtgacg ggatcgcgtc gtggtgctca gcgccagccg gtccccgtgg
     3421 acgccatcat gacgccgtca ttggggccgc tcgaggaacc ttgccgatcg cttcgagacg
     3481 agccaggatc ggatgcaccg tgccgctcgg caacccc
//
LOCUS       DAOPJG010000266         3476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_30251_length_3476_cov_8.515931, whole genome shotgun sequence.
ACCESSION   DAOPJG010000266 DAOPJG010000000
VERSION     DAOPJG010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30251_length_3476_cov_8.515931"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PKU75_16050"
     CDS             <1..882
                     /locus_tag="PKU75_16050"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPF82439.1"
                     /translation="AALLELREQARSGRWELIVVDCAPTAETLRLLAFPENLAWHLRR
                     VLPGTAGMRRAARPAAAAALGIPLPGREIVEILRRWHDDMLAVRHLLASATSSVRLVT
                     TPESVVIAESRRTWTSLSLYGYAVDAVYVNKILPAPALKEGDGAAAQWLRSWNAAQTR
                     GIDEVVQSFVGVPVVRSPYLEREPIGPAALAELHGQLDLGTLAGRDITKPATAEPMQV
                     RRTDAGFDLALRLPLTSAKEVGLQRRADELLLTVGDHRRIVTLPSVLSRCTVADARVR
                     SGHLTIAFVPDPAQWPR"
     gene            879..1211
                     /locus_tag="PKU75_16055"
     CDS             879..1211
                     /locus_tag="PKU75_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82440.1"
                     /translation="MSDTTDPAGSLAEEARRLADALGGPAAAASEGHPATCTWCPLCR
                     GVDSLRAVDPAAIDRLAAAVSALAGALGDISQHLREQFAPGVGGAPAPNAGGDRTAYD
                     IPVSDEED"
     gene            1212..2177
                     /locus_tag="PKU75_16060"
     CDS             1212..2177
                     /locus_tag="PKU75_16060"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HPF82441.1"
                     /translation="MALAIGVDIGGTKVAAGVVDTSGQVLRRARADTPERSVRPQVVE
                     DTIVGVVTELIESCAEPIVAIGIGAAGFVAADRATIVFSPHLSWRNEPLRDRLSDRIG
                     QPIFIDNDANAAMWAEAKYGAVRGETHAMMITLGTGIGGGILMGGEVIRGLFGIAGEF
                     GHMQVVPGGHRCECGNRGCWEQYASGNALVREARGLVAANSPLARDLYERVAGDPARL
                     TGPLVTEVAQAGDRTAIELLAEVGTWLGIGIANLAAAFDPGAFVIGGGVSAAGELLLD
                     PARQAFRKQLTGRGFRPEARIVRAELGNEAGLIGAADLARESVAG"
     gene            2178..2891
                     /locus_tag="PKU75_16065"
     CDS             2178..2891
                     /locus_tag="PKU75_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPF82442.1"
                     /translation="MSRLRIASYNTRDFLDDRAAAARLIRAIDPDVLCLQEVPRRLFA
                     PQRVAAFAAECGLTWSGHHRGSGGTTIFTSLRVQVVESKHFRLRVKLAQRTRGFALAR
                     VAIPGREPIAVASVHLSLDAAERAVHAREILRATGTTDTVLCGDLNEQSDGAAWQLIA
                     TPLRLISPTKPTHPSWGPRRLLDVIFASPGIEPLPHVDIALDPEDMSMASDHRPVWVD
                     VRTGPDDSSPSDRSDHGAA"
     gene            complement(2906..3460)
                     /locus_tag="PKU75_16070"
     CDS             complement(2906..3460)
                     /locus_tag="PKU75_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82443.1"
                     /translation="MPEPDPLPPEDDLDARFEEIVAQLRAEQPSTPGDALTVRDLDDL
                     PGAGDPTAGLPPPADPAPPRDAVNPPPVFRPAPPPPRGLDVPVWRGASGDAAYDELLD
                     QIEAEDHYEPPPPRPLPPQQDLHFWGIIIGLVGGPLILLWLVLFRPDVSDLWGWAGAG
                     MTIGGFVLLVLRGDDHDRGDGAVV"
BASE COUNT          462 a         1270 c         1270 g          474 t
ORIGIN      
        1 gccgccctgc tggagctgcg ggagcaggcc cggtcgggcc gctgggaact gatcgtcgtc
       61 gactgcgcgc cgaccgccga gacgctgcgg ctgctcgcct tccccgagaa cctcgcctgg
      121 cacctgcgcc gggtcctgcc cggaaccgcc ggtatgcggc gcgccgcccg gcccgctgct
      181 gccgctgccc tcggcatccc cctgccgggg cgggagatcg tcgagatcct gcgccgctgg
      241 cacgacgaca tgctcgcggt gcggcacctg ctcgccagcg ccacctcgtc ggtgcgcctc
      301 gtcaccaccc cggagagcgt cgtcatcgcc gagtcccgcc ggacctggac ttcgttgagc
      361 ctctacgggt atgccgtgga cgccgtttac gtgaacaaga tccttcccgc gccggctctg
      421 aaggagggcg acggcgcggc ggcgcagtgg ctgcgctcgt ggaacgctgc ccagacccgg
      481 ggcatcgacg aagtggtcca gtccttcgtc ggcgtaccgg tcgtgcgttc gccctatctg
      541 gagcgggaac cgatcgggcc ggcggccctg gccgagctgc acggccagct ggacctgggg
      601 acgcttgccg gccgcgacat cacgaagccg gccacggccg aaccgatgca ggtgcggcgc
      661 accgacgccg ggttcgatct ggcgctgcgg ttgccgctca cgagcgccaa ggaggtcggc
      721 ctgcagcggc gggccgacga actgctgctc accgtcggcg atcaccggcg catcgtgacg
      781 ctgccgtcgg tgctgagccg atgcacggtg gccgacgcgc gggtccgttc ggggcatttg
      841 acgatcgctt tcgtgcccga ccccgcacaa tggcctcgat gagcgacacc accgacccgg
      901 ccggatccct tgccgaagag gcccgccggc tcgccgacgc cctcgggggc ccggccgcgg
      961 cggcatccga agggcacccg gccacgtgca cgtggtgccc gttgtgccgg ggcgtcgact
     1021 cgttgcgggc ggtggacccc gcggcgatcg accggctggc tgccgcggtg agcgccctcg
     1081 ccggcgctct cggcgacatc tcgcagcacc tgcgcgagca gttcgcgccc ggggtgggcg
     1141 gggcgccggc accgaacgcc gggggcgacc gcacggcata cgacatcccc gtgagcgacg
     1201 aggaggacta gatggcgctg gccattggcg tggacatcgg cggcaccaag gtggccgcag
     1261 gggtggtgga cacctccggg caggtcctgc ggcgtgcgcg agccgacacc cccgaacgtt
     1321 cggtccggcc ccaggtggtc gaggacacga tcgtcggtgt cgtcaccgaa ctcatcgaga
     1381 gttgcgccga accgatcgtc gccatcggga tcggcgccgc gggattcgtg gccgccgacc
     1441 gggccaccat cgtcttctct ccgcacctgt cctggcgcaa cgagcccctg cgcgaccgcc
     1501 tgagcgaccg gatcggccag ccgatcttca tcgacaacga cgccaatgcc gccatgtggg
     1561 ccgaggcgaa gtacggcgcc gtgcggggcg agacccacgc gatgatgatc accctcggca
     1621 ccggcatcgg cggcggcatc ctcatgggcg gcgaggtgat ccgcgggctg ttcgggatcg
     1681 cgggggagtt cggccacatg caggtcgtgc ccggcggcca tcgctgcgag tgcggcaacc
     1741 gcggatgctg ggagcagtat gcctccggca acgcgctcgt gcgcgaggcg cgcgggctcg
     1801 tggccgccaa cagcccgctg gcgcgggacc tgtatgaacg tgtcgcggga gacccggcgc
     1861 gcctgaccgg tccgctggtc accgaggtgg cccaggccgg cgatcgcacc gccatcgagt
     1921 tgctcgccga ggtcggcacc tggctcggca tcggcatcgc gaacctcgcg gcggcgttcg
     1981 acccgggggc cttcgtcatc ggcggcggcg tcagcgccgc cggcgagttg ctgctcgacc
     2041 cggcccggca ggccttccgc aaacagctga ccgggcgcgg cttccggccg gaggcacgga
     2101 tcgtgcgggc cgaactcggc aacgaggcgg ggctgatcgg tgcggccgac ctggcccgcg
     2161 agagcgtggc cggctgagtg tcccggctgc gcatcgcgag ctacaacacc cgcgatttcc
     2221 tcgacgaccg cgcggctgct gcccggctca tccgggccat cgacccggac gtgctgtgcc
     2281 tgcaggaggt gccgcggcgg ctgttcgcgc cgcagcgggt ggccgccttc gctgccgagt
     2341 gcgggctgac ctggtcgggg caccaccgcg gcagcggagg cacgacgatc ttcacctcgc
     2401 tgcgggtgca ggtcgtggag tccaagcact tccggctgcg ggtcaagctg gcgcagcgga
     2461 cgcgcggctt cgcgctcgcc cgggtcgcga tccccgggcg ggaaccgatc gccgtcgcgt
     2521 ctgtgcacct cagcctcgac gccgccgaac gggccgtcca cgcccgcgag atcctgcgcg
     2581 caaccggcac gaccgacacc gtcctgtgcg gtgacctcaa cgaacagtcc gacggcgccg
     2641 cctggcagct gatcgccacc ccgctgcggc tgatctcccc gaccaagccg acccatccct
     2701 cttgggggcc gcggcgcctg ctcgacgtca tcttcgcctc accgggcatc gaaccgctgc
     2761 cgcacgtgga catcgcgctc gaccccgagg acatgtcgat ggccagcgac caccgccccg
     2821 tgtgggtcga cgtccgcacc ggcccggacg actcgtcacc gtcggatcgg agtgaccacg
     2881 gagcggcttg acctcggggg atccgtcaga ccaccgcgcc gtcgccgcgg tcgtggtcgt
     2941 cgccgcgcag gaccagcagc acgaacccgc cgatcgtcat accggcgccg gcccaacccc
     3001 acagatcgga gacgtcgggg cggaacagca cgagccacag caggatgagg gggccgccga
     3061 cgaggccgat gatgatcccc cagaagtgca gatcctgctg cggcggcagt gggcgcgggg
     3121 gcggcggctc gtagtggtcc tccgcctcga tctgatccag cagttcgtcg tatgccgcgt
     3181 caccgctcgc gccgcgccat accgggacgt cgagcccccg cggcggcggg ggagcaggcc
     3241 ggaagaccgg gggcgggttg accgcgtccc gcggcggggc gggatcggcg ggcggcggca
     3301 gacccgccgt cgggtcgccg gcgcccggga ggtcgtcgag gtcgcgcacg gtgagcgcgt
     3361 cgcctggggt gctcggctgc tcggcacgca gttgggccac gatttcctcg aaccgcgcgt
     3421 cgaggtcgtc ctcgggcggc agcggatcgg gctcgggcac gccttatcct cgcacg
//
LOCUS       DAOPJG010000267         3460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_30456_length_3460_cov_5.302790, whole genome shotgun sequence.
ACCESSION   DAOPJG010000267 DAOPJG010000000
VERSION     DAOPJG010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30456_length_3460_cov_5.302790"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..864
                     /locus_tag="PKU75_16075"
     CDS             355..864
                     /locus_tag="PKU75_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82444.1"
                     /translation="MTSPERGILARRDTLLLAPAALLGLATPALAEDDERLSTITGIM
                     ACNRAARWVDADVPYSQTTYYNGYRTDCSGYVSFAWGLARPGLTTWSLRRVGDFIHKP
                     QLRRGDALLDPNRHVVLFDKWADAGHTSYWGYEESSSQGAVHRIIPYPYWPGYGTYKP
                     FHKRGMTPA"
     gene            complement(1401..1787)
                     /locus_tag="PKU75_16080"
     CDS             complement(1401..1787)
                     /locus_tag="PKU75_16080"
                     /inference="COORDINATES: protein motif:HMM:NF022963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HPF82445.1"
                     /translation="MSAYESTGEFTIEMTPAEGLIEGTSRFDFSKLWTGAVDGTSAGV
                     MMSGGDPAVGEAGYVAMERFEGTVDGRAGSVAFQQIGAMSGGEASMSYAVVPGSGTHE
                     LAGMEGEIDLDTAEGHRVTLRYTIPD"
     gene            2143..2826
                     /locus_tag="PKU75_16085"
     CDS             2143..2826
                     /locus_tag="PKU75_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPF82446.1"
                     /translation="MASPRHTRRTVTIVESDGTTTALNEAGMSLPAEELADVLATLRE
                     LLATSAHGSVLVLSGSLPPGTPDHAYADCVAAARDHGIPAIVDCSGPALLAALDAGAA
                     LVKPNRHELLATVGADGVIGAQRLRKRAPDAVVVASDGPAGLLCVGPDGTWRATPARA
                     LVGNPTGAGDALVAALAVGMARGDDLPTMLRAAVGSSGSAVLDPVAGHIDPAVAADLA
                     AQATLSRID"
     gene            3168..>3460
                     /locus_tag="PKU75_16090"
     CDS             3168..>3460
                     /locus_tag="PKU75_16090"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPF82447.1"
                     /translation="MGIPSDLEIASAASLRPLHEIADRLGIPQDFVEPYGRSVAKIDL
                     NVLTADGQPREQRAKYVVVTAVTPTPLGEGKTTTAVGLADGLARIGQTVAVTLR"
BASE COUNT          539 a         1270 c         1135 g          516 t
ORIGIN      
        1 caagccctgg ccgtcaaggg gtgagcccgt tgagctgagc accaggtcgg tggtggccag
       61 cactgggcag atcccatggc cacccccggg cagaactcgt gaccgtcacc gggcaggttt
      121 cacgaccgcc cctgggcaga aaccaatggc ccttgacagg aatgaccggc ccgaacagcg
      181 gggccacgtt ttcgcatgcg ggccgggtta tgacccggcc cgcatgcgag aagccggccc
      241 gggtcgacgg cgccccgacg ttgacagcca tacaggaccg gccgttaccc tcgggtcacc
      301 tcgtcgcggc ggcgaagcac gacgccggcc gcgctgcccc ccggaggatc ccgcatgacc
      361 agccccgaac gcggcatact cgcgcgccgc gacactctcc tgctcgcccc cgccgccctg
      421 ctcgggctgg cgacaccggc cctggccgag gacgacgagc gcctttcgac gatcaccggc
      481 atcatggcct gcaaccgcgc cgcgcggtgg gtggacgccg atgtgccgta cagccagacg
      541 acctattaca acggctaccg caccgattgc tcgggctatg tctccttcgc ctggggtctg
      601 gcccgccccg gcctgacgac gtggtcgctg cgccgggtcg gcgacttcat ccacaagccg
      661 caactgcgcc gcggggacgc cctcctcgac cccaaccggc acgtcgtgct cttcgacaag
      721 tgggccgacg ccgggcacac gtcctactgg gggtatgagg agtcctcctc ccagggcgcc
      781 gtgcaccgga tcatccccta tccctactgg cccggttacg gcacctacaa gcccttccac
      841 aagcggggta tgacgcccgc ctgacggacg agcacaccgc ggaccggtaa gtgccgggct
      901 cctcaccgat acccgcggct acttccaggc gctcacggac tgccgtctcg gtgccgtcga
      961 cccgatcgtc gcctgcgtcg ccgaagccct catcatcggc gtcgatcgcg cacgggccct
     1021 catcgccgat ctcgacaccg tccatgccga gaggcaggac cagctcaaag ggatccgccc
     1081 cctccggggc caggaggctg gccgacgagc cgcttcgctg tccggtcatc cccgccgccg
     1141 aagcgcgcga catcctcggc gccacactgt ccaccggcac ctggacgcat tggtcgcgcg
     1201 aggcatcctg atcccacacc aggatgacaa gagccgcaat acgacctggc gcgcccctga
     1261 cgttctcgcc gcactcgatc gattcgccga cgggggatgc cgcgcgtgat caacgctgtc
     1321 ggcgctactc ccaccgtgca gcactcctcc cacgtcccga tgtgggagta ataccaggag
     1381 gggcgatcgc ggaccgggcg tcagtccggg atggtgtagc gcagcgtcac gcggtggccc
     1441 tcggcggtgt cgagatcgat ctcgccctcc atccctgcca gctcatgggt gccggagccg
     1501 gggaccacgg catacgacat gctcgcctcg cccccggaca tggcaccgat ctgctggaag
     1561 gccaccgacc cggcccgccc gtcgacggtg ccctcgaagc gttccatggc cacgtagccg
     1621 gcctcgccga ccgccgggtc gccgcccgac atcatcacgc cggcgctcgt gccgtcgact
     1681 gcgccggtcc agagcttgct gaaatcgaag cgcgacgtgc cttcgataag cccctcggcc
     1741 ggcgtcatct cgatcgtgaa ctcgcccgtg ctctcgtatg ccgacatggc ggcctcctcc
     1801 cgtgtgcgga caccgtagcg acatcgtgcc cgtcgtgaag ctgaaagggg ccggggcaac
     1861 tgcgacggca tacggttgtc gcgtgacggt cacggaagac ccggcgatcc tgtgcctcgg
     1921 gctcaacccc gccctcgaca tcacctatcg actcgatcgg ctcgatcacg gcgagtcgca
     1981 gaaggtcacg gacgtgcgcg agcgcgcggg agggaaggcg acgaacgtcg cgcgcgtcgc
     2041 cgctcaagtc ggcggggcca gtcatctgct cgcagcgctg ggtggtcccg tcggggcggc
     2101 catcgccgac gaactgtatg ccggtggagc cggttcacgc gggtggcgag cccacggcat
     2161 acccgccgca ccgtcaccat cgtcgagtcc gacggcacga ccaccgcgct caacgaggcg
     2221 ggcatgtccc tgcccgcgga ggaactcgcg gatgtcctgg ccacgctgcg cgaactgctg
     2281 gccacctcag cgcacggctc ggtgctggtg ctcagtggtt ccttgccgcc gggcacaccc
     2341 gatcacgcgt atgcggattg cgttgcggca gcgcgcgatc acggcatacc ggcgatcgtc
     2401 gactgctcgg gaccggccct tttggccgcg ctcgacgcgg gggcggctct ggtgaagccg
     2461 aaccgtcacg aactcctcgc gaccgtgggc gcggacgggg tgatcggcgc gcaacggctc
     2521 cgcaagcgtg cgcccgacgc ggtggtcgtg gcctccgacg ggccggccgg actgctctgc
     2581 gtcgggccgg acggcacgtg gcgagccaca cctgcccgag ccttggtcgg caacccgacc
     2641 ggagctggag acgctctcgt ggccgcgctc gccgtcggca tggcacgggg tgacgacctg
     2701 cccacgatgc tgcgggccgc tgtcggtagc tccggctctg ccgtgttgga tcccgttgcg
     2761 ggacatatcg atccggccgt cgctgccgac ctggccgccc aggcgacact gtcccggatc
     2821 gattgatcgg caccaagagg ccttgcgtaa cctagtagtt acctacccaa taggcacttt
     2881 gcgatccgtg gacgccgacg cctggctcga cgactatcga gccctgtggc agcaccggct
     2941 cgatgcgctg cataccgggc atcatcgagg cctgactgac gcccgaggag cacgggaccc
     3001 ggctcgtggc ggaggagcgc ggcataccgc tggggccgtc ccggcgtatc ggtccgcctg
     3061 gcgggtgcac ctcgacgacc tgggtcgctt gacctctcga cgaccaagaa ctgaccgctc
     3121 gacgggtgag aagtgaccgg tcgacggggg gcggcatact cgctggtatg ggtatcccct
     3181 ccgacctcga gattgccagt gccgccagtc tccgtccgct ccacgagatc gcggaccgac
     3241 tcggcatccc ccaggacttc gtcgagccgt atggccgcag tgtcgccaag atcgacctga
     3301 acgtcctgac cgccgacgga cagccgcgcg aacagcgcgc gaagtatgtc gtggtcaccg
     3361 ccgtcacccc gacgccgctg ggcgagggca agacaaccac cgccgtaggc cttgccgacg
     3421 ggctcgcccg gatcgggcag acggtcgcgg tgacgctgcg
//
LOCUS       DAOPJG010000268         3413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_31007_length_3413_cov_7.119714, whole genome shotgun sequence.
ACCESSION   DAOPJG010000268 DAOPJG010000000
VERSION     DAOPJG010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31007_length_3413_cov_7.119714"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="PKU75_16095"
     CDS             <1..1663
                     /locus_tag="PKU75_16095"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPF82448.1"
                     /translation="ENISKVLYPEDSTPQGKELRLQQQYFFVACSIFDFLKHTQPTDL
                     DVRELPERIIFQLNDTHPVIGIPELMRLLIDDYDLTFEEAWAITKQCFAYTCHTLLPE
                     ALEVWPVSLMERLLPRHMEIIYQINEVFLAEVREQFPGDELRVRRMSIIGDHPERSVR
                     MAHLATVASVKVNGVAELHSQLLRDKVLADFSEMWPEKFTNVTNGVTPRRFVRQANPF
                     LSKLITDTIGVGWVANLERVRELETYAEDPEFRAKFREVKAANKTRIAAVLERRDGIL
                     LPEDHLLDVMVKRLHEYKRQMLKLLHIVTLYEGIISGDLDPATVTPRTVVFGAKAAPG
                     YRMAKEIIFLINSVASVINNDPRVQGKLFIAFPANYNVTLAERLIPAADLSEQISLAG
                     KEASGTGNMKFALNGALTIGTDDGANVEIRKLVGDDNFFLFGMTEPEVADLQARGYRP
                     TEYYESNEKLRRAINLIDSGHFTGGNREAASMVVGDLLYNDRFLVLADYQAYLDAQAE
                     VEAAYADQEAWSRSAILNVARTGFFSSDRSMRDYINRIWHIQTGF"
     gene            complement(1802..2167)
                     /locus_tag="PKU75_16100"
     CDS             complement(1802..2167)
                     /locus_tag="PKU75_16100"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82449.1"
                     /translation="MAKDPAAKIIVAGDVNDFEFSLTTDILVDILEGSRTKALTDLPR
                     TLPAHQRWTYVYEGNSQVLDHILISNSLASLEKFSGPYRRGSGVKPAFVYDIVHTNAP
                     FFDQDSDHDPQVVHLDLKR"
     gene            2166..3329
                     /locus_tag="PKU75_16105"
     CDS             2166..3329
                     /locus_tag="PKU75_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82450.1"
                     /translation="MISSTKPRTHPGLRVAGRLAGAHRLLPAPEQRVIAALGREVHGR
                     HEDGLATELELAHDRLARVVEGRVGRVDPAGADAQRWPPLLLRDDLRVRRRARGAGID
                     EGQVAVEAVEQDVADVAAGLAAVRLVLRVDPLAGVCRSAGSLDGRNELRHRLISRDGA
                     VVGGTAVVLDLLDREDVRAAQVVHDLAGEAGELLVGILRCKVLDVEGGHGQLAVVRLG
                     DGRLARQTARRVRRHGRQFDLEVGEAVVHDAGAQTRKGVSHTGFRHLVSTDDQVVELQ
                     AHGVRPVVAAVDDAVELAADRLDFAAGYHGDLAERGRRTDNLRVADTDVHPLEGLVEV
                     DGVAVGQEGDPVVFDAADLLGLDRLLRGDAVLADDHRGRHGVAARDRSTKSLL"
BASE COUNT          561 a         1130 c         1143 g          579 t
ORIGIN      
        1 cgagaacatc tccaaggtgc tctaccccga ggactccacg ccgcagggca aggagctgcg
       61 gctgcagcag cagtacttct tcgtcgcctg ctcgatcttc gacttcctca agcacaccca
      121 gccgaccgac ctcgacgtgc gggaactgcc cgagcggatc atcttccagc tcaacgacac
      181 ccaccccgtc atcggcattc ccgagctgat gcggctgctc atcgacgact acgacctgac
      241 gttcgaggaa gcgtgggcga tcaccaagca gtgcttcgcc tacacctgcc acacgctgct
      301 tcccgaggcg ctggaggtct ggcccgtctc gctgatggag cggctgctgc cgcggcacat
      361 ggagatcatc taccagatca acgaggtctt cctcgccgag gttcgcgagc agttcccggg
      421 cgatgagctg cgggtgcgcc ggatgtcgat catcggcgac caccccgagc gctcggtgcg
      481 gatggcgcac ctggcgacgg tggcgtcggt caaggtcaac ggcgtggccg aactgcatag
      541 ccagctgctg cgggacaagg tgctcgcgga cttctccgag atgtggccgg agaagttcac
      601 caatgtcacc aacggggtca ccccccggcg gttcgtgcgg caggccaacc cgttcctgtc
      661 caagctgatc accgacacca tcggggtcgg gtgggtggcc aatctcgagc gggtgcgcga
      721 gctcgagacg tatgccgagg acccggagtt ccgcgcgaag ttccgcgagg tcaaagccgc
      781 caacaagacg cggatcgccg ccgtcctcga gcgccgcgac ggcatactgc tgcccgagga
      841 tcacctgctc gacgtgatgg tcaagcggct gcacgagtac aagcgccaga tgctcaagct
      901 gctgcacatc gtcaccctct acgagggcat catctccggt gacctcgacc cggcgaccgt
      961 caccccgcgg accgtcgtct tcggcgccaa ggccgcaccc ggctaccgga tggccaagga
     1021 gatcatcttc ctgatcaact cggtcgccag cgtcatcaac aacgacccgc gcgtgcaggg
     1081 caagctgttc atcgccttcc cggccaacta caacgtcacc ctcgccgagc ggctgatccc
     1141 ggccgccgac ctctcggagc agatctccct ggccggcaag gaggcctccg gcaccggcaa
     1201 catgaagttc gcgctgaacg gggccttgac gatcggcacc gacgacggtg ccaacgtcga
     1261 gatccgcaag ctggtcggcg acgacaactt cttcctcttc gggatgaccg agcccgaggt
     1321 cgcggacctg caggcccggg gctaccggcc gacggagtac tacgagtcca acgagaagtt
     1381 gcgccgcgcg atcaacctga tcgactcggg gcacttcacc ggcggcaacc gcgaggcggc
     1441 cagcatggtc gtcggcgacc tgctctacaa cgaccgtttc ctcgtgctcg ccgactacca
     1501 ggcctatctg gacgcgcagg cggaggtcga ggcggcatac gccgaccagg aggcgtggag
     1561 ccggtcggcc atcctgaacg tcgcccggac cggcttcttc tcgtccgacc gctcgatgcg
     1621 cgactacatc aaccggatct ggcacatcca gaccggcttc tgacgtgggc ggcgtcgggt
     1681 tggcgccaac agccttggtt agcgccaaca accttggtta gcgcgaactg cgccggaaac
     1741 cgcggactcc cccagatatt tctgggggag tccgcggttt ccggggtggt tcgcgcttgg
     1801 ctcagcgctt gaggtccagg tggacgacct gggggtcgtg gtcggagtcc tggtcgaaga
     1861 agggggcgtt ggtgtggacg atgtcgtaga cgaacgccgg cttcacgcca ctcccccggc
     1921 ggtaggggcc ggagaacttc tcgagtgagg ccagtgagtt ggagatcagg atgtggtcga
     1981 ggacctggct gttgccctcg tagacatacg tccagcgctg gtgcgcaggc aaggtccgcg
     2041 gcagatcggt gagggccttg gtgcgcgagc cctccaggat gtccacgagg atgtcggtcg
     2101 tcaggctgaa ctcgaagtcg ttgacgtcgc cggcgacgat gatcttggcg gcgggatcct
     2161 tggccatgat ctcgtcgacg aaaccgcgga cacaccctgg cctgcgcgtg gcgggccggc
     2221 tcgctggagc gcaccggctg ctgccagcgc ccgaacagcg ggtcatcgcc gcccttggac
     2281 gagaagtgca cggccgccac gaagacggtc tggccacgga acttgaactg gcccacgacc
     2341 ggcttgcgcg agtcgtcgaa ggccgggttg gtcgggtcga tccggccggg gctgatgctc
     2401 aacgatggcc gcccctgctt ctccgtgacg accttcgtgt ccgtcgtcgc gctcggggtg
     2461 ccggcattga cgaaggtcag gtcgcggtcg aggcggtaga gcaggacgtt gcggatgttg
     2521 ccgccgggct ggccgccgtc cgccttgtcc tgcgggtcga cccacttgcc ggcgtatgcc
     2581 ggtccgccgg cagcctggat ggccgcaacg agcttcgcca ccgtctgatc agccgcgacg
     2641 gtgccgtcgt cggtgggacc gctgttgtcc tggacctcct cgatcgcgag gatgtccggg
     2701 ctgcgcaggt tgttcacgat ctggccggcg aggcgggcga acttctcgtc gggattctgc
     2761 ggtgcaaggt tctcgacgtt gaaggaggcc acggtcagct ggcggtcgtc cgtcttggcg
     2821 acggccgtct cgcgcgtcaa accgctcggc gcgtgcgccg gcacggccgt caattcgacc
     2881 ttgaagttgg cgaagctgta gtccacgacg ccggtgctca aacccggaag ggtgtctccc
     2941 acactggctt tcggcatctt gtcagcaccg atgatcaggt cgtcgagttg caggcgcatg
     3001 gagttcggcc ggtcgtagcc gccgtagacg acgccgttga actcgctgcg gatcgcctcg
     3061 actttgctgc cgggtaccac ggggatctcg ccgaacgagg ccgtcggacc gacaacctcc
     3121 gcgtcgcgga caccgacgta catcccctcg agggactcgt agaagtcgat ggcgtcgcgg
     3181 tcgggcagga aggcgacccc gtcgtattcg acgctgccga cttgctcggc ctcgatcgtc
     3241 tgctgcgggg cgacgcggtc ctcgccgatg accaccgggg caggcacggc gttgccgctc
     3301 gagaccgctc gacgaagtcg ctcctgtagg gggcgccgga gttgccgccg ccgccatccg
     3361 cctcggcgat gacgacattc tcggaggccg cggaggcgga cggcgcgcag acg
//
LOCUS       DAOPJG010000269         3379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_31466_length_3379_cov_6.657942, whole genome shotgun sequence.
ACCESSION   DAOPJG010000269 DAOPJG010000000
VERSION     DAOPJG010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31466_length_3379_cov_6.657942"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKU75_16110"
     CDS             <1..419
                     /locus_tag="PKU75_16110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF82451.1"
                     /translation="FGSSVDGAEPNEDWLALGDGSPVGLVQCSAVADYEENLADFSAL
                     GEVPPRAITLDYLLGETRGQGLGTDMLRAFVARCWAERPEAPAVLLTVVAANRPSWRA
                     LERVGFRIVGEGDCELDNPDDDPLHYLLRLDRPGVA"
     gene            416..985
                     /locus_tag="PKU75_16115"
     CDS             416..985
                     /locus_tag="PKU75_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82452.1"
                     /translation="MIVLVEGASDAAAVRAGAVGRGISLSRIDIRPMGGVTNVRKALA
                     AAYETTPEAQVIGLCDAGESAVVERALRERGQPVRDAGDLPAYGWFVCVRDLEEELIR
                     ALGPTRTLAVIDDLGLTTKLRTLLGQPQWAGRPLEEALHRFCGVASGRKELLAGALAV
                     ALTAGEEPEPLRLLLDWLGSGDWAMREEG"
     gene            1014..1436
                     /locus_tag="PKU75_16120"
     CDS             1014..1436
                     /locus_tag="PKU75_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HPF82453.1"
                     /translation="MPKTTKSGKPIEDELPSPVQRSSTRAQETFAKTYDSAMEQYDDE
                     GRANATAYASLKRTHERVGDKWVAKKESGPSDERAEEGGLNDKESAGGVDANATKTHL
                     LDVAKRLKIRGRTRMTKDELVEAIRTANDKANEKARER"
     gene            complement(1537..2064)
                     /locus_tag="PKU75_16125"
     CDS             complement(1537..2064)
                     /locus_tag="PKU75_16125"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HPF82454.1"
                     /translation="MTTRIAFLRAVNVGKRRVTSDQLRAVCEDLGYADVGTYINSGNA
                     HFETTGSRASIEQAMGEALEKELGFECTTFVRTLNELKKALSAKPFDVADGDTYFITF
                     LKDPPTAAQKSALEGLSGDIDTLVVLGRDVHWRMHGTSMESALTKRHWEKILGPLSST
                     SRNTTMLTKLAARLG"
     gene            complement(2061..2660)
                     /locus_tag="PKU75_16130"
     CDS             complement(2061..2660)
                     /locus_tag="PKU75_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPF82455.1"
                     /translation="MAGQRTRAYGIPLPPPRERVDRFEECIEVISRLRTGERITFDGK
                     HIQLRDAIVSPTPRATLPLLVGTNGPRMLRITARFADRWNTWGLPDQVGPRSAALTEA
                     CEREGRDPGGIRRSAQALVRLTPDPAERDAFLEEHGGRGFAGSAEELIDAFGRYAEAG
                     IDEFGIHTIAFSDDPDRRHDELDRFADEVLDRVATKAQR"
     gene            complement(2573..2980)
                     /locus_tag="PKU75_16135"
     CDS             complement(2573..2980)
                     /locus_tag="PKU75_16135"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPF82456.1"
                     /translation="MDFSLWAFSGRPFARTLADARWAADRGLRGLWLADHLMANTPDD
                     TSARDPISECWTALAAIAALVPRIELVSHVFPTTWHHPVVLLKRVIEVDQISEGRAVL
                     GLGAGWQVNEHAPTAYRSRHHANASTASRSASR"
     gene            3139..>3379
                     /locus_tag="PKU75_16140"
     CDS             3139..>3379
                     /locus_tag="PKU75_16140"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPF82457.1"
                     /translation="MGMQGTPCWFELDTTDLDASTAFYGQILGWQVADSGMPDADYRL
                     ASAPDGAMVAGLSSTDEQEQASPKWVVYFAVDDADA"
BASE COUNT          506 a         1109 c         1274 g          490 t
ORIGIN      
        1 atttcgggtc gagtgtcgac ggggccgagc cgaacgagga ctggctggcc ctaggcgacg
       61 gctcgccggt cgggctcgtc cagtgctccg cggtggccga ctacgaggag aacctggccg
      121 acttctcggc gctgggggag gtgcctcccc gggcgatcac cctcgactac ctcctcggcg
      181 agacccgcgg ccaggggctc ggcaccgaca tgctgcgagc gttcgtcgcg cgctgctggg
      241 ccgagcgccc ggaggcgccg gccgtcctgc tgaccgtcgt cgccgccaac cggccgtcct
      301 ggcgggcgct ggagcgcgtg ggttttcgca ttgtgggaga aggggattgc gagctcgaca
      361 acccggacga cgacccactc cactacctcc tccgcctcga ccggccaggt gtcgcgtgat
      421 cgtgctcgtc gagggtgcca gcgacgcggc ggccgtgcgc gccggcgcgg tcggccgcgg
      481 gatcagcctc tcccgcatcg acattcgccc catgggtggg gtgaccaacg tgcggaaggc
      541 gcttgcggcg gcatacgaaa cgacgccgga ggcgcaggtg atcggcctgt gcgacgcggg
      601 cgagagcgcc gtcgtcgaac gcgcgctgcg tgagcgcggg cagccggtgc gcgacgccgg
      661 cgacctgccg gcatacggct ggttcgtctg cgtgcgcgac ctggaggagg aactgatccg
      721 cgcgctcggc cccacgcgca ccctcgccgt catcgacgac cttggcctca cgacgaaatt
      781 gcgcacgctg ctcgggcaac cgcagtgggc cggccggccc ctcgaggagg cactgcaccg
      841 gttctgcggg gtggcctccg ggcgcaagga actgctcgcg ggcgcgctgg ctgttgccct
      901 caccgccggg gaggaacccg agccgctgcg gctgctgctc gactggctcg gatcgggcga
      961 ctgggcgatg cgcgaggagg gttagggggc ggctcggcgg ctaccgtcga gatatgccga
     1021 agacgaccaa gagcggcaag cccatcgagg acgagttgcc cagtcccgtc cagcgctcca
     1081 gcacgagggc acaggagacc ttcgccaaga cgtatgactc ggcgatggag caatacgacg
     1141 acgaggggcg ggccaacgcg acggcatacg cctcgctgaa gcgcacccac gagcgcgtcg
     1201 gcgacaagtg ggtggcgaag aaggagtccg gaccctcgga cgagcgggcc gaggagggcg
     1261 gcctcaacga caaggagtcc gcgggcgggg tcgacgcgaa cgcgacgaag acgcacctgc
     1321 tcgacgtggc gaagcggctg aagatccgcg ggcggacccg gatgaccaag gacgagctcg
     1381 tcgaggccat ccgcacggcc aacgacaagg cgaacgagaa ggcgcgcgag cgctgaggcc
     1441 ggcgccgcat cacgcggcac catccgtcca tgggcctgcg gcgacggtat gacgtgctgg
     1501 tcgccggcca ccgtgcgcac ccgcgaggcg cctcgatcag cccaggcgcg cggccaattt
     1561 ggtgagcatc gtggtgttgc ggctggtgct gctgagcggc ccgaggatct tttcccagtg
     1621 ccgtttggtg agcgcggact ccatcgacgt gccgtgcatg cgccagtgga cgtcgcggcc
     1681 cagcacgacg agggtgtcga tgtcgccgga cagtccctcg agggccgact tctgggccgc
     1741 ggtgggcgga tccttgagga aggtgatgaa gtaggtgtcg ccgtcggcga cgtcaaaagg
     1801 cttggcggac aacgctttct tgagttcgtt gagggtgcgc acgaaggtgg tgcactcgaa
     1861 acccagctcc ttctcgagcg cctcccccat ggcctgctcg atcgacgcgc gactcccggt
     1921 ggtctcgaag tgggcgttgc cgctgttgat gtaggtcccc acgtcggcat agccgaggtc
     1981 ctcgcagacg gcccgcagtt ggtcggaggt gacgcgccgc ttgcccacgt tgaccgcccg
     2041 caggaaggcg atccgggtgg tcaccgctgg gccttcgtgg cgacgcggtc gaggacctcg
     2101 tcggcgaacc ggtcgagttc gtcgtggcgg cggtccgggt cgtcgctgaa cgcgatcgtg
     2161 tggatgccga actcgtcgat accggcctcg gcatagcgcc cgaaggcgtc gatcagctcc
     2221 tccgccgacc ccgcgaaccc gcgccccccg tgctcctcga ggaaggcgtc gcgctcggcc
     2281 gggtccggcg tcagccgcac cagcgcctgg gcgctgcgtc gtatgccgcc cgggtcgcgc
     2341 ccctcgcgct cgcaggcctc ggtcagggcc gcgctgcgtg gtcccacctg gtcgggcagg
     2401 ccccaggtgt tccatcggtc ggcgaatcgt gctgtgatgc gcagcatccg cgggccgttg
     2461 gtgccgacga ggagcgggag ggtcgctctc ggggtggggg agacgatggc gtcacgcagc
     2521 tgaatgtgct tgccgtcgaa ggtgattcgc tcgccggtgc gcagccgcga gatcacctcg
     2581 atgcactcct cgaagcggtc gacgcgttcg cgtggtggcg ggagcggtat gccgtaggcg
     2641 cgtgttcgtt gacctgccag ccggccccga ggccgaggac cgcgcggccc tcgctgatct
     2701 ggtcgacctc gatgacccgc ttgagcagga cgaccgggtg atgccaggtg gtggggaaga
     2761 cgtgggagac cagttcgatg cgcggcacca gggcggcgat ggcggcgagc gcggtccagc
     2821 actcgctgat cggatcgcgg gccgaggtgt cgtccggcgt gttggccatc aggtggtcgg
     2881 cgagccacag ccctcttagc ccgcggtcgg cggcccaccg cgcgtccgcg agggtccggg
     2941 cgaacggccg gccggagaag gcccacaggg agaagtccat ggggccgatt cttccgcacc
     3001 cggtccgcat acccgcgggc gcggagcggc ggcggaatcc ggcggcgggt gaacagcgac
     3061 tggaatctcg gacggagcgg gacggctgtc ggtgcccggc gctagtgtcc gaaggaccac
     3121 gaccgagggg atgacacgat gggtatgcaa ggcacgccgt gctggttcga actggacacc
     3181 accgacctcg acgcgagcac cgcgttctac ggccagatcc tcggttggca ggtcgccgac
     3241 tcggggatgc ccgacgcgga ctaccggctc gcatcagcgc ccgatggggc gatggtggcg
     3301 ggtctgtcga gcaccgacga gcaggagcag gcgtcaccga agtgggtggt ctacttcgcg
     3361 gtcgacgacg ccgacgcga
//
LOCUS       DAOPJG010000270         3379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_31467_length_3379_cov_6.517750, whole genome shotgun sequence.
ACCESSION   DAOPJG010000270 DAOPJG010000000
VERSION     DAOPJG010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31467_length_3379_cov_6.517750"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /gene="rpsD"
                     /locus_tag="PKU75_16145"
     CDS             <1..550
                     /gene="rpsD"
                     /locus_tag="PKU75_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPF82458.1"
                     /translation="GGDKNFDLRPFPPGQHGRRRIQEKEYLTQLQEKQRARFTYGVME
                     KQFRRYYEEAANRPGQTGHNLLTILESRLDNVIYRAGLARTRRHARQLVTHGHFEVNG
                     VRVDVPSYRVEQYDIITIRKQSVESFPFQLARETFGERPVPAWMQVVPGTLQILIHQL
                     PVREQIDSQLTEQLIVELYSKN"
     gene            771..1796
                     /locus_tag="PKU75_16150"
     CDS             771..1796
                     /locus_tag="PKU75_16150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPF82459.1"
                     /translation="MLIAQRPLLTEEVVAENRSRFVIEPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSIRIDGVLHEFSTIPGVKEDVTEIILNIKGLVVSSEEDEPVVMYLRKQGP
                     GAVTAADIAPPAGVEIHNPDLHIATLNDKGKLEMELTVERGRGYVSAQQNKSGEQEIG
                     RIPVDSIYSPVLAVTYKVEATRVEQRTDFDKLIVDVETKNSMAPRDAMASAGKTLVEL
                     FGLARELNVEAEGIEMGPSPTDAALAADLALPIEDLELTVRSYNCLKREGIHTVGELV
                     GRSEADLLDIRNFGTKSIDEVKAKLHSMGLGLKDSPPGFDPSALGDRYDSYDEDDDLS
                     FAEDEQL"
     gene            1836..>1973
                     /locus_tag="PKU75_16155"
     CDS             1836..>1973
                     /locus_tag="PKU75_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HPF82460.1"
                     /translation="MPTPKKGPRLGGGPAHERLILANLATALFEHDSITTTEAKAKRL
                     RP"
     assembly_gap    1974..1983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1984..2752
                     /gene="rplQ"
                     /locus_tag="PKU75_16160"
     CDS             <1984..2752
                     /gene="rplQ"
                     /locus_tag="PKU75_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPF82461.1"
                     /translation="PLAERLVTFAKRGDLHARRRVMTVVRDKGVVHRLFVEIAPDMAE
                     RQGGYTRITKIAPRKGDNAPMAVIELVREPVSAKPKRAVVAEAEGATKRAAKEKKAKD
                     APVAAAPVEDVVVEDMVAEDVPVAEAEVQDAPVVEDAPVVEDAPAPVVAEDAAVDSAA
                     PAASQNAPVADLTDAEPVAATQAAALGEVAEAPADGSQPVGFDIKGNKDSMLYHVPGS
                     QWYDATEAEVWFDTADAAEKAGFQPAGGAEKQQVDEA"
     gene            2886..3353
                     /locus_tag="PKU75_16165"
     CDS             2886..3353
                     /locus_tag="PKU75_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82462.1"
                     /translation="MVDVEIAAPRQALEEHGTPLALAIADALGRMAETDLDPDERAGI
                     AAIEARRAALLEDNSFVAGSETLTVHDATRLASKKVREARLLFRLVRAIKPKVAIELG
                     TNMGISGAYQALALKLDGDGRLITLEGHPAGRRSPRARSSPTATPSTEPTRRR"
BASE COUNT          592 a         1224 c         1082 g          471 t
ORIGIN      
        1 cggcggcgac aagaacttcg acctgcgtcc cttcccgccc ggccagcacg gccgccgccg
       61 catccaggag aaggagtacc tcactcagct ccaggagaag cagcgcgccc gcttcaccta
      121 tggcgtcatg gagaagcagt tccgtcgcta ctacgaggag gcggccaacc gccccggtca
      181 gacgggtcac aacctgctga ccatcctcga gtcccgcctg gacaatgtca tctaccgcgc
      241 cggcctggcc cggacccgcc gccacgcgcg tcagctcgtc acgcacggcc acttcgaggt
      301 caacggcgtg cgcgtggacg tcccgtccta ccgcgtcgag cagtacgaca tcatcacgat
      361 ccgcaagcag tcggtcgagt ccttcccgtt ccagctggcg cgggagacct tcggcgagcg
      421 tccggtcccg gcctggatgc aggtcgtccc gggcacgctg cagatcctca tccaccagct
      481 gcccgttcgc gagcagatcg actcccagct caccgagcag ctgatcgtcg agctctactc
      541 caagaactag cccttcgggc tgaggtcaag gaacctctgc tagcagaagt tagtgaaatc
      601 agcacaccta gcacggtctg cagatttgac caacctctgc tagcagaggt tccttggccc
      661 ggccctgggc ggccccgccc agcggtgggg agcggcggcg gacataccga ggtccggcgc
      721 cggcatacga agacatcaaa tagcgggtgt cttcgggaaa gaagaaaaca gtgctgatcg
      781 cacagcgtcc cctcctgacc gaggaggtcg tcgccgaaaa ccgcagccgg ttcgtcatcg
      841 agccgctcga gcccggtttc ggctacaccc tcggcaactc cctgcgccgc acgctcctgt
      901 cgagcatccc cggcgccgcc gtgaccagca tccgcatcga cggggtgctg cacgagttct
      961 ccacgatccc cggggtcaag gaggatgtca ccgagatcat cctcaacatc aagggcctgg
     1021 tcgtctcctc cgaggaggac gagcccgtgg tgatgtacct gcgcaagcag ggtccgggcg
     1081 ccgtcacggc cgccgacatc gccccgcccg ccggtgtgga gatccacaac cccgacctgc
     1141 acatcgcgac cctgaacgac aagggcaagc tcgagatgga gctgaccgtc gagcgcggcc
     1201 gcggctatgt ctcggcgcag cagaacaagt ccggtgagca ggagatcggc cgcatcccgg
     1261 tcgactccat ctactccccg gtgctggccg tcacctataa ggtcgaggcc acccgtgtcg
     1321 agcagcgcac cgacttcgac aagctgatcg tcgacgtcga gaccaagaac tcgatggcgc
     1381 cgcgcgacgc gatggcctct gccggcaaga cgctcgtcga gctgttcggc ctggcccgtg
     1441 agctcaacgt cgaggccgag ggcatcgaga tgggcccgtc gccgacggat gccgctctcg
     1501 ccgccgacct ggcactgccg atcgaggacc tcgagctgac cgtccgctcc tacaactgcc
     1561 tcaagcgcga gggcatccac accgtgggtg agctcgtcgg ccggtccgag gcggacctgc
     1621 tggacatccg caacttcggc accaagtcca tcgacgaggt caaggccaag ctgcatagca
     1681 tgggtctggg cctgaaggac agcccgcccg gattcgaccc gtccgccctc ggcgaccgct
     1741 acgactccta cgacgaggac gacgacctgt ccttcgccga ggacgagcaa ctgtaaaagc
     1801 cggctctgag cgaccacatc acctaggaga aatcaatgcc tacccccaag aagggtcccc
     1861 gcctcggcgg cgggccggct cacgagcggc tgatcctcgc caacctggct accgcccttt
     1921 tcgagcacga ctcgatcacg acgaccgagg ccaaggccaa gcgcctgcgc ccgnnnnnnn
     1981 nnncccgctc gccgagcgcc tcgtcacctt cgccaagcgc ggtgacctgc acgcccgccg
     2041 ccgcgtgatg accgtcgtcc gcgacaaggg cgtcgtccac cgcctcttcg tcgagatcgc
     2101 tcccgacatg gccgagcgcc agggcggcta cacgcgcatc accaagatcg cgccgcgcaa
     2161 gggcgacaac gcccccatgg ccgtgatcga actcgtccgc gagcccgtct cggccaagcc
     2221 gaagcgcgcc gtcgtcgccg aggccgaggg cgcaaccaag cgcgcggcca aggagaagaa
     2281 ggccaaggac gctcccgtgg ccgccgctcc ggtcgaggac gtcgtggtcg aggacatggt
     2341 ggccgaggac gttccggtcg ccgaggccga ggtgcaggac gcccccgtcg tcgaggacgc
     2401 tccggtcgtc gaggacgccc cggctccggt cgtggcggag gacgcagccg tcgattctgc
     2461 tgcccctgcc gccagccaga acgcgccggt cgctgacctg accgacgccg agcccgttgc
     2521 cgccacccag gctgccgcgc tcggcgaggt cgccgaggcg cccgccgacg ggtcgcagcc
     2581 ggtcggcttc gacatcaagg gcaacaagga ctcgatgctc taccacgtcc ccggttcgca
     2641 gtggtatgac gccaccgagg ccgaggtctg gttcgacacc gccgacgccg ccgagaaggc
     2701 gggcttccag cccgccggtg gcgccgagaa gcagcaggtc gacgaggcct gagccacctc
     2761 gttctgctcc gaagccccga cccgccatcg cgcgggccgg ggcttcggcg ttcgctgcgt
     2821 cataccctcc ccggcgcgcc cgtcgctacc gggatcggca cagccgctcg tgtacgttgc
     2881 tgagcgtggt cgacgttgag attgcggccc cgcggcaggc gctggaagag catggcaccc
     2941 ccctcgccct ggcgatcgcc gacgccctcg ggcggatggc cgagacggat ctggatcccg
     3001 acgagcgggc cggaatcgcg gccatcgagg cgcggcgggc agcgctgctc gaggacaaca
     3061 gtttcgtcgc cgggagcgaa accctgaccg tgcacgacgc gacgcggctg gcgagcaaga
     3121 aggtccggga ggcgcggctg ctgttccgcc tggtgcgcgc catcaagccg aaggtggcca
     3181 tcgaactcgg gaccaatatg ggcatcagcg gcgcctatca ggcgctggcc ctgaagctcg
     3241 acggcgatgg ccggctgatc accctcgaag gccacccggc cgggcggcgg tcgcccaggg
     3301 cacgatcgag ccctacggca acaccgagca ctgagcccac tcgtcgccgg tgagtgggct
     3361 gcggatccgg atcgatctc
//
LOCUS       DAOPJG010000271         3360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_31731_length_3360_cov_7.197579, whole genome shotgun sequence.
ACCESSION   DAOPJG010000271 DAOPJG010000000
VERSION     DAOPJG010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31731_length_3360_cov_7.197579"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1185
                     /gene="prfB"
                     /locus_tag="PKU75_16170"
     CDS             70..1185
                     /gene="prfB"
                     /locus_tag="PKU75_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPF82463.1"
                     /translation="MATDFTQEIKDLRSTMTSVREVTDLDALGAKIADLEAKAGAPDL
                     WDDPESAQKVTSSLSRAIAERDRVQELDQRVDDLEVLVELGEEEGDEATLAEAERELE
                     SIRGAVGELEVRTLMSGEYDEREAVVTIRSGAGGVDAADFAEMLLRMYLRWAEKHGYP
                     TKVLDTSYAEEAGLKSATFEVNVPYAYGNLSVEGGTHRLVRISPFDNQGRRQTSFAAV
                     EVIPLIESTDHIEIPENEIKVDVFRSSGPGGQSVNTTDSAVRMTHIPTGIVVSMQDEK
                     SQIQNRAAALRVLQSRLLLLRKAEEAATKKELAGDIKASWGDQMRSYVLQPYQMVKDL
                     RTGYEVGNPQAVFDGDVDGFIEAGIRWRRSGAEASGE"
     gene            complement(1333..>3360)
                     /locus_tag="PKU75_16175"
     CDS             complement(1333..>3360)
                     /locus_tag="PKU75_16175"
                     /inference="COORDINATES: protein motif:HMM:NF016047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HPF82464.1"
                     /translation="RMPPTTPLRPALVIAGVTLAVTGATAIPSAADAGAPGADRDRLV
                     ATATARQAFTPSADALTITTSAGASTYSYDAASGELGDLIATTPGSAGQRWSPGGDVV
                     LDGGATWTTRGRDDAQLGSYARNAKALMPGWLTTGDGFVEQSPGATTPSTAISLTPGE
                     RWRQPVAAGAVNYGPFAVSPYGTEAVVRGIKTGSSNLYLVPVRGPMTLAGQPWTFPTR
                     DYLVSSYRPGDADVAQEPGKGIRDSSARTYIAFLGTEPGSSEASLFVDYQDGGRHLGE
                     PLQPTRVIGAGSRCTTTAPAFSPDRRSIAYVVGVGSSANPCSQMAVRVVSLGSGGSYD
                     AATVRTLHTAPVGAPIRAVSWRAVTAPVQQVRIGGEDRYEVGVNVSRSVYGDAGAGGA
                     VIAGGEAYADALAGSPLSAALKGPLMLTRKDRVDARVVAELNRVLKAGAAIYVLGGPA
                     TVGEGVLTELRAQTGHPVVRIGGASRYDVAVAVAKVLATVRGEQAAAIFVANGQVFPD
                     ALVASPAATVTRGVILLSAEDALPAATKTYIDAHRGVALYGIGGGGGRSLAGYSGVEV
                     ISGADRYEVAKRVADRFFTAEWIGTVVSGLNWPDATTGGALISQWRQPILLARGATLP
                     SWTAERLDSSRAAIDLLAVFGGPASVDSAAMDEAVRRAGSQTLYHGPDSPR"
BASE COUNT          507 a         1127 c         1266 g          460 t
ORIGIN      
        1 cggtgcgggg gcgggcggca gcggcgacgc gagcaggcgt cggtcgccgc tgccgtaggc
       61 ttgctccttg tggctacgga cttcacccag gagatcaagg acctgcgctc gacgatgacg
      121 tcggtgcgcg aggtgaccga tctggacgct ctcggcgcca agatcgccga tctcgaggcg
      181 aaggccggcg ctcctgatct gtgggatgac ccggagagcg cccagaaggt cacctcgagc
      241 ctgtcgcggg ccatcgccga gcgcgaccgc gtgcaggaac tcgaccagcg ggtcgacgac
      301 ctcgaggtcc tcgtcgaact cggggaggag gagggtgacg aggcgacgct ggcggaggcc
      361 gagcgggaac tcgagtcgat ccgtggcgcc gtgggtgagc tcgaggtgcg cacgctgatg
      421 tcgggggagt atgacgagcg cgaagcggtc gtgacgatcc gctccggcgc cggcggcgtc
      481 gatgcggcgg acttcgccga gatgctgctg cggatgtatc tgcgttgggc ggagaagcac
      541 ggctacccga cgaaggttct cgacacctca tacgccgaag aagccggcct gaagtcggcc
      601 accttcgagg tcaacgtccc ttacgcctac ggcaacctct cggtcgaggg cggcacccac
      661 cgcctggtgc ggatcagccc cttcgacaac cagggccgcc ggcaaacctc cttcgccgct
      721 gtggaggtca tcccgctcat cgagtccacc gaccacatcg agatccccga gaacgagatc
      781 aaggtggacg tcttccgctc ctcgggtccc ggcgggcaga gcgtcaacac caccgactcg
      841 gcggtgcgga tgacgcacat cccgaccggc atcgtcgtct ccatgcagga cgagaagtcc
      901 cagatccaga accgcgccgc ggcccttcgc gtgctgcagt cgcgtctgct gctgctgcgc
      961 aaggccgagg aggccgcgac caagaaggaa ctcgccggcg acatcaaggc cagctggggc
     1021 gaccagatgc gctcgtatgt gctgcagcca tatcagatgg tcaaggatct gcgcaccggc
     1081 tacgaggtgg gcaaccccca ggctgtcttc gacggcgacg tcgacggctt catcgaggcc
     1141 ggcatccggt ggcgccgctc gggcgccgag gcgtcgggcg agtagggctc gacagcggct
     1201 ctcgggggtg cttcagcggg gtccccgcga agtatcggag cgacgaagga gcggagaact
     1261 tcggggggtg cgtcagcggg gtccccgcga agtatcggag cgacgaagga gcggagaacg
     1321 tcggggggtg cttcagcggg gggagtcggg gccgtggtag agggtctggc tgcccgcgcg
     1381 gcggaccgcc tcgtccatgg ccgcggagtc gaccgaagcc ggtccgccga agacggcgag
     1441 caggtcgatc gcggcgcgtg aggagtccag gcgctccgcg gtccacgacg gcagggtcgc
     1501 gccccgggcc agcaggatcg gttgccgcca ctggctgatc aaggcaccgc cggtggtggc
     1561 gtcgggccag ttcagcccgg agacgaccgt gccgatccat tcggcggtga agaagcgatc
     1621 ggcgacgcgc ttggcgacct cgtagcggtc ggccccgctg atcacctcga ctccggagta
     1681 gccggcgagg ctgcggccac cgccaccgcc gatgccgtag agcgcgacac cgcggtgggc
     1741 gtcgatatac gtcttcgtcg ctgcaggcag ggcgtcctcg gcgctgagca ggatgacgcc
     1801 gcgggtcacc gtcgcggccg ggctcgccac gagagcgtcg gggaagacct gtccattggc
     1861 gacgaagatg gcggccgcct gctcgccccg cacggtggcg aggactttcg cgacggcgac
     1921 ggcgacgtca taccggctcg ccccgccgat gcgcacgacc gggtgaccgg tctgggcgcg
     1981 cagctcggtc agcaccccct cgccgacggt cgccggaccg ccgaggacgt agatcgcggc
     2041 gccggccttg agcacgcggt tcaactcggc gacgacgcgc gcatcgacgc ggtccttgcg
     2101 ggtcagcatg agcggaccct tcaacgccgc cgagagcggt gagccggcca gcgcatcggc
     2161 atacgcctcg ccgcccgcga tcaccgcgcc accggcgccc gcgtcgccat acacactgcg
     2221 cgagacgttg acaccgacct cgtagcgatc ctcgcccccg atccgcacct gctggaccgg
     2281 ggcggtgacg gcgcgccacg agacagccct gatcggcgct cccacggggg cggtgtgcag
     2341 cgtgcgaacc gtcgctgcgt catacgatcc tccggacccg aggctgacga cgcgcacggc
     2401 catctgcgag cacgggttgg cgctggaccc gacgccgacg acgtaggcga tgctgcgccg
     2461 gtcgggactg aacgcaggag ccgtcgtcgt gcaccgcgac ccggcgccga tgacgcgggt
     2521 gggctgcagc ggttcgccga ggtgtcgccc gccgtcctga tagtcgacga aaagggacgc
     2581 ctcgctcgag cccggctcgg tgccgaggaa ggcgatatag gtgcgggcgc tggagtcgcg
     2641 gatgcccttg ccgggttcct gcgcgacgtc cgcgtccccg ggccggtagc tcgaaaccag
     2701 gtagtcgcgg gtcgggaagg tccacggctg cccggccagc gtcatcgggc cgcgcaccgg
     2761 caccagataa aggttggagg agccggtctt gatgccgcgg accacggcct cggtgccgta
     2821 ggggctgacg gcgaacggcc cgtagttgac ggcgccggcc gcgaccggct gccgccagcg
     2881 ctccccgggc gtcagcgaga tcgccgtcga gggggtggtg gcgccggggc tctgctcgac
     2941 gaagccgtcg ccggtggtga gccaccccgg catgagcgcc ttggcgttgc gggcgtagct
     3001 gccgagttgg gcgtcgtcgc gtccgcgcgt ggtccaggtc gccccgccgt cgagcaccac
     3061 gtccccgccg ggcgaccagc gttgacccgc ggagccgggg gtggtggcga tcaggtcgcc
     3121 gagttcgccg gaggccgcgt cgtaggagta ggtgctcgcg cccgccgagg tcgtgatcgt
     3181 cagggcgtct gcgctcggcg tgaacgcctg cctggccgtg gccgtcgcga cgagccggtc
     3241 acggtcggct cccggcgcac ccgcgtcggc ggccgacggt atggcagtgg ctccggtcac
     3301 cgcgagggtg acaccggcga tgacgagggc gggacgcaac ggcgtggtgg ggggcatgcg
//
LOCUS       DAOPJG010000272         3350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_31867_length_3350_cov_5.211836, whole genome shotgun sequence.
ACCESSION   DAOPJG010000272 DAOPJG010000000
VERSION     DAOPJG010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31867_length_3350_cov_5.211836"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKU75_16180"
     CDS             <1..561
                     /locus_tag="PKU75_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HPF82465.1"
                     /translation="WLGLEQSELDVSAWQIAKSVLIFLGIPLAAGYLSRRIGEQRWGR
                     AAYERSFVPRIGPWALYGLLFTIVILFALQGDQITSRPLDVARIALPLLAYFALMWGG
                     GYLLGKALGLTYERTTTLAFTAAGNNFELAIAVAIATFGVASGQALAGVVGPLIEVPV
                     LVGLVYVSLALRSKYPHQPTALPATR"
     gene            complement(551..1801)
                     /gene="rocD"
                     /locus_tag="PKU75_16185"
     CDS             complement(551..1801)
                     /gene="rocD"
                     /locus_tag="PKU75_16185"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPF82466.1"
                     /translation="MTNAPTPSRTDQVIAATERWAAHNYHPLPVVLATGDGAWVTDVE
                     GRRFLDCLAGYSAVNFGHGNPRLLRVAHEQLDRLTLTSRAFYNDQLGGFAQALTALTG
                     KDMCLPMNSGAEAVETALKVARKWGYRTRGIPEGAATIIVMEGNFHGRTTTIVSFSDD
                     KVAHDEYGPYTPGFVTVPYGDADALAAALQTNEFTVAVLLEPIQGEGGVVIPPKGYLR
                     RVRELCSGANALMMADEIQSGLARTGETFACDHEGVVPDVYILGKALGGGIMPLSAIA
                     CDHEIMDVITPGSHGSTFGGNPFACAIGTEVCAMLETGEFQAAARERGAQLAAGLEPL
                     LGAGLTAYRQRGLWAGLDLDPTVGTGRELCERLVAHGILAKDTHGSTIRLAPPLVATS
                     EDIELLLGAVSAGLADMTGSGVSA"
     gene            complement(1861..2583)
                     /locus_tag="PKU75_16190"
     CDS             complement(1861..2583)
                     /locus_tag="PKU75_16190"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HPF82467.1"
                     /translation="MTLAVVAGLIGLALVDSTSLGTLVMPVWMIAHPRLRPSRVVLYL
                     ATIALFYWALGLALLLGASALTARWEEIGASRTTSWVQLVIGVGMLVASFWPDTPWAR
                     RRARERATGWREAGWRGRVAGADARASTVVTVALLAGLVEAASMVPYLGAIGLLSTSG
                     LTAPASGAVLAVYVAVMCLPALVLLGLRLGVAERVTPLLTRLEAWLTTHTGGAIWWVV
                     GILGFFLAADAVTRLGLWGPGS"
     gene            2676..>3350
                     /locus_tag="PKU75_16195"
     CDS             2676..>3350
                     /locus_tag="PKU75_16195"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPF82468.1"
                     /translation="MPKIVDHAARRSEIAHALWRVIARDGLEGASVRVVAAEAGWSLG
                     AVRHYFATQDELVEFATDLMIDGVRERLLAHFDMEPGMPRAQALLEELLPLDERRVGE
                     VNVWLALLGRARREPRLAQLRLDAWEAERYLCRIAVSDVLGRPTPGPNALLEEDLESV
                     AAQLHTVIDGLTIHGVTVPEKAGAADLCNQLRQALLAIADRPAPAPEPVIEAGCFACG
                     NTAQGDA"
BASE COUNT          526 a         1192 c         1107 g          525 t
ORIGIN      
        1 tggctcggcc tggagcagtc cgagctcgac gtctccgcgt ggcagatcgc caagagcgtc
       61 ctgatcttcc tcggcatccc cctcgccgcc ggctacctgt cccgacggat cggcgaacag
      121 cggtggggga gagcggcata cgagcgctcc ttcgtgccgc gcatcggccc ctgggcgctc
      181 tacggcctgc tgttcacgat cgtcatcctc tttgcgctgc aaggcgacca gatcaccagc
      241 cgtcctctcg acgtcgcccg gatagccctc ccgctgctgg cctacttcgc gctgatgtgg
      301 ggtggcggtt acctcctggg caaggccctc ggcctgacgt acgagcgcac cacgaccctg
      361 gctttcacgg cagcgggcaa caacttcgag ttggccatcg ccgtcgccat cgccaccttc
      421 ggcgtcgcct ccggccaggc cctcgccggc gtcgtgggtc ccctcatcga ggtgcccgtc
      481 ctcgtcggcc tggtctacgt cagtctggcc ctgcgctcga agtacccgca ccagccaacc
      541 gcactcccgg ctacgcgctg acgcccgatc ccgtcatgtc ggccaggccc gccgacacgg
      601 caccgaggag cagctcgatg tcctcggagg tcgccaccag cggcggagcg aggcggatgg
      661 tcgagccgtg cgtgtccttg gccagaatcc catgtgccac aaggcgttcg catagctcgc
      721 gtcccgtgcc gacagtcggg tccagatcga ggccggccca caggccgcgc tgccggtatg
      781 ccgtgaggcc ggcccccagg agcggctcga gtcccgctgc caactgggcg ccacgctcgc
      841 gcgccgctgc ctggaactcg ccggtctcga gcatggcgca gacctcggtg ccgatggcgc
      901 acgcgaatgg attccccccg aaagtcgagc cgtgcgagcc gggcgtgatc acgtccatga
      961 tctcgtgatc gcaggcgatc gcggagagtg gcatgatgcc gccgccgaga gccttgccga
     1021 ggatgtacac atccggcacg accccttcgt ggtcgcatgc aaacgtctct ccggtgcggg
     1081 cgagcccgga ttggatctcg tcggccatca tcaatgcgtt tgcgccggaa cacaattcgc
     1141 gcacgcgccg gagatagccc ttcggtggga tgacgacacc cccttcgccc tggatcggtt
     1201 ccagcaagac ggcgacggtg aactcgttgg tctgcagcgc cgccgccagc gcgtcggcat
     1261 cgccataggg aacggtgacg aaacccggtg tgtagggacc gtattcgtcg tgggcgacct
     1321 tgtcgtcgga gaaagagacg atcgtcgtgg tccggccgtg gaagttgccc tccatgacga
     1381 tgatcgtcgc cgcgccttcg gggatcccgc gcgtgcgata cccccacttg cgagccactt
     1441 tcaacgcggt ctcgacggct tcggccccgg agttcatcgg caggcacatg tccttgccgg
     1501 tcaatgccgt cagcgcctgc gcgaaccccc cgagctggtc gttgtagaac gcccgcgacg
     1561 tcagcgtgag gcggtcgagt tgctcgtgcg cgacccgaag caggcgcggg ttgccgtgcc
     1621 cgaagttcac ggccgaatag ccggccaggc agtcgaggaa gcgccgcccc tcgacgtcgg
     1681 tcacccaggc cccgtcaccg gtggccagca ccaccgggag agggtggtag ttgtgggcgg
     1741 cccagcgttc cgtcgctgcg atgacctgat cggttcggga cggcgtcggc gcgttcgtca
     1801 tcgatggctc cttcgtatcg gctcgtggcc gacccccacg ctagcgaacc gcaccggccc
     1861 tcaggagccc ggaccccaca agcccagtcg cgtgacggcg tctgcggcaa ggaagaagcc
     1921 gagaatgccg accacccacc agatcgcgcc gcccgtatgc gtcgtcagcc atgcttcgag
     1981 ccgggtcagc agcggggtga ctcgttcggc cacgccgagt cgcaacccca gcaggaccag
     2041 cgccggcagg cacatgaccg ccacatacac cgcgagcacg gctcccgatg cgggggctgt
     2101 caagccgctg gtcgacagca gcccgatcgc cccgagatac ggcaccatcg aggccgcctc
     2161 caccagtcct gccaacaggg cgacggtgac cacggtcgag gctcgggcgt ccgcaccggc
     2221 cacccgtccc cgccatccgg cttcgcgcca gcccgtggcc cgctctcggg cccgccgtct
     2281 cgcccacggc gtatcgggcc agaagctggc caccagcatg ccgacaccga tgacgagttg
     2341 cacccaactg gtcgtccggc tggcgccgat ctcctcccat cgagccgtca gcgccgacgc
     2401 gcccagcagc agcgccagcc cgagggccca gtagaagagc gcgatcgtcg cgagatagag
     2461 caccacccgc gacggtcgca ggcgcggatg ggcgatcatc cacaccggca tgacgagcgt
     2521 gccgaggctg gtgctgtcga ccagcgccag cccgatcaga cctgcgacga cagccagagt
     2581 cattcgcgtc ccccttggca ctacgtccgt ttgacaatac gcccgtgctt taaccgaccg
     2641 taggggattc aatacggtcg tgtcaaaata ggtccgtgcc gaagatcgtc gaccacgccg
     2701 cgcgtcgcag cgagatcgct catgccctgt ggcgggtcat cgcccgcgac ggtctcgagg
     2761 gcgccagtgt ccgggtcgtc gccgccgagg cggggtggtc cctcggggca gtgcgccact
     2821 acttcgccac gcaggacgaa ctggtcgagt tcgccacgga cttgatgatc gatggcgtgc
     2881 gcgagcgcct cctcgcgcac ttcgacatgg agccgggtat gccgcgggcg caagctctcc
     2941 tggaggagct gctcccgctg gatgagcggc gggtcggcga ggtcaacgtg tggctggctc
     3001 tcctgggccg ggcgaggcgt gagccgcggc ttgcccagct gcgtctggac gcgtgggaag
     3061 ccgaacgcta tctgtgccga atcgccgtca gcgatgtcct gggcaggccc acgccgggac
     3121 cgaatgctct gctcgaggag gacttggaat cggttgcggc ccaactgcat acggtgatcg
     3181 acgggctgac catccacggg gtgaccgtgc ccgagaaggc gggcgccgcg gacctgtgca
     3241 atcaattgcg ccaggcgttg ctggccattg ccgaccggcc cgcgccggcg cccgagccgg
     3301 ttatcgaagc gggctgcttc gcgtgcggca acaccgccca gggggacgca
//
LOCUS       DAOPJG010000273         3346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_31923_length_3346_cov_7.103008, whole genome shotgun sequence.
ACCESSION   DAOPJG010000273 DAOPJG010000000
VERSION     DAOPJG010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31923_length_3346_cov_7.103008"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKU75_16200"
     CDS             <1..366
                     /locus_tag="PKU75_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dioxygenase subunit beta"
                     /protein_id="HPF82469.1"
                     /translation="SGRWPHIHFEVYPDQASITDSTNAIATSQIAVPKDACDMVYATS
                     GYEDSVTNLAQLSLATDNVFGDDSGATQLATMSGDVDAGYTIALTAAVDTSTEPTGGS
                     APGGDGQPGGAPGGTASTS"
     gene            complement(427..1317)
                     /locus_tag="PKU75_16205"
     CDS             complement(427..1317)
                     /locus_tag="PKU75_16205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPF82470.1"
                     /translation="MSDPLRGHRFEVDVEGGARLIGEVYAPAGHIDADTPTVVLAHGW
                     TLNRTLWSRVIRRLHDRLPVRIVAYDQRSHGESTRGNLAASIGQLSDDLAAVIEVVAG
                     ADRLVLCGHSMGGMTIISYAGRHTEELHDRVDGVALLGTACAEVGRPGWFGKFEAGVM
                     HAARWGPAIPAGAFVTSRHQRFLNFGDDPDPRDVRLVRKAIASTKIRDMGSYFGALEA
                     LDERESLAALAAVPTVIMVGEKDRLTPVHYAQALHEGIDGSALMVLPGKGHMLGYEAT
                     DDVVDVLAGLVQGNPIEVPA"
     gene            complement(1310..2095)
                     /locus_tag="PKU75_16210"
     CDS             complement(1310..2095)
                     /locus_tag="PKU75_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPF82471.1"
                     /translation="MRIATWNVNSIRSRIERVAAWLERSDIDVLAIQETKTRVDQFPF
                     FRFEDLGYEVAHHGFDQWNGVAIASRVGMADVRAGFDGAPTWREATEPEARHVAATCG
                     GIRVNCVYVPNGRHLTDPHMDYKIAWLDALRVAAEGWITDGPSMIMGDWNIAPRDEDV
                     WSMEFYEDKSHVSPRERAAFEAVVASGYADVVRPFLPGPKVYTYWDYQQLRFQKGRGM
                     RIDFLLASPDIASRVTGASIDREERKGKGASDHAPVIVEVADV"
     gene            2160..2927
                     /locus_tag="PKU75_16215"
     CDS             2160..2927
                     /locus_tag="PKU75_16215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPF82472.1"
                     /translation="MTTALITGPTAGIGAEFARQLAAQGHDLVLVARDQPRLEATAEG
                     LRAAYGCAVAILVADLSVRDQLQRVADRLADPARSVDILVNNAGFGLKGGFLDNDLAQ
                     EEELFDVLCRAVLVLSHTAARAMRERGSGRIINVSSVASFIAGSSYSAAKSYVTVLTE
                     ALSVALAGSGVTATALCPGFVHTEFHERAEMGMDYLPDMAWVPVDKLVREALLDSEKG
                     KVISVPGWQYKAATRILKLTPRALARHPRIVGQHRKP"
     gene            2984..>3346
                     /locus_tag="PKU75_16220"
     CDS             2984..>3346
                     /locus_tag="PKU75_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPF82473.1"
                     /translation="MTDIAADRAALLEHIKAKAIVHGRVTLSSGKEADYYVDLRRITL
                     DGEAAPLVGRVMRELVKDLDFDAVGGLTLGADPVATSMLHAAAAAGQRLDAFVVRKSG
                     KAHGLQRRIEGPSIAGRRV"
BASE COUNT          537 a         1162 c         1138 g          509 t
ORIGIN      
        1 tccggccggt ggccgcacat ccacttcgag gtctatccgg atcaggcgag catcaccgac
       61 agcaccaacg ccatcgcgac ctcgcagatc gccgtcccca aggacgcgtg cgacatggtg
      121 tacgccacca gcggctacga ggactcggtc acgaacctcg cccagctgtc gctcgcgacg
      181 gacaacgtct tcggagacga ctccggcgca acgcaattgg ccacgatgag cggcgacgtc
      241 gacgcgggct acacgatcgc tttgacggcc gccgtcgaca cgagcaccga accgaccggc
      301 ggctcggccc ccggcggcga cggccagccc ggtggggcgc cgggaggcac cgcgtccacg
      361 agttgatgga gcgcccgttg ggccggtgga gtcaactgct cagtcggccg gtctcgtgga
      421 cggcggtcag gcgggcacct cgatggggtt gccctggacg agaccggcga gcacatccac
      481 gacgtcgtcc gtcgcctcgt aacccagcat gtggcccttg cccggcaaca ccatcagcgc
      541 cgagccgtcg atgccttcat gcagggcctg ggcatagtgg acgggagtca gtctgtcctt
      601 ctctcccacc atgatcaccg tcggcaccgc ggcgagagcg gccaaggact cgcgctcgtc
      661 gagcgcttcc agggcgccga agtaggagcc catgtcccgg atcttggtgc tcgcgatggc
      721 cttgcggacc aggcgcacgt cgcgcgggtc ggggtcgtca ccgaagttga ggaaacgctg
      781 gtgacgggag gtgacgaagg cgccggcggg gatggccggg ccccaccggg ccgcgtgcat
      841 gacaccggct tcgaacttgc cgaaccaccc cggccggccc acctcggcgc atgcggttcc
      901 gagcagcgcc accccgtcga cccggtcgtg cagttcctcc gtgtgccggc cggcgtagga
      961 gatgatggtc atgccgccca tcgaatgccc gcacaggaca agacgatccg cgcccgccac
     1021 gacctcgatg acggcggcga ggtcgtcgct gagctgcccg atcgaggctg ccaggttccc
     1081 ccgcgtcgac tcgccgtggc tgcgctggtc ataggcgacg atccgcaccg gcagccggtc
     1141 gtggagacgc cggatgaccc ggctccacag cgtgcggttg agcgtccacc cgtgcgcgag
     1201 gacgaccgtg ggagtgtcgg cgtcgatgtg tccggccggc gcgtagacct cgccgatcaa
     1261 ccgggcgcca ccctcgacgt cgacctcgaa ccggtgcccg cgcaagggat cagacatcgg
     1321 ccacctccac gatcacgggc gcatgatcgg atgccccctt gcccttgcgt tcttcgcggt
     1381 cgatcgatgc gccggtgacc cgggaggcga tgtccgggct ggccagcagg aagtcgatcc
     1441 gcatcccacg gcccttctgg aagcgcaact gctgatagtc ccaataggtg taaaccttgg
     1501 gcccggggag gaaggggcgc accacatcgg catacccgga cgcgacgacc gcctcgaatg
     1561 cggcccgctc gcgggggctg acgtgcgact tgtcctcgta gaactccatc gaccagacgt
     1621 cctcgtccct cggtgcgata ttccaatctc ccatgatcat gctcgggccg tctgtgatcc
     1681 agccctctgc cgcgacgcgc aatgcatcga gccaggcgat cttgtagtcc atgtgggggt
     1741 cggtcaggtg ccgcccgttg ggcacgtaga cgcagttgac ccggatgccg ccgcaggtcg
     1801 ccgcaacgtg cctggcctcg ggttctgtgg cctcgcgcca cgtgggagcg ccgtcgaaac
     1861 cggcgcgcac atcggccatt ccgacgcgcg aggcgatggc gacgccattc cactggtcga
     1921 agccgtggtg agccacctca tagcccaagt cctcgaaccg gaagaagggg aactggtcga
     1981 cccgggtctt ggtctcctgg atggcgagca cgtcgatatc ggagcgctcc agccaggcgg
     2041 ccacgcgctc gatccgggag cggatggagt tgacgttcca ggtggcgatc cgcacggcct
     2101 cacagtaggc cagccggccc acgcggggca tcccaggcgg ggtggctagg gtcacgtcca
     2161 tgacgacggc tctgatcacc ggcccgacgg cgggcatcgg cgccgagttc gcgcgccagc
     2221 tcgcggcgca ggggcatgat ctggtcctcg tagcccgcga ccagccccgc ctggaggcga
     2281 ccgcggaggg gttgcgggcg gcatacggct gcgccgtggc gatcctcgtg gccgacctgt
     2341 ccgttcgcga ccagctgcaa cgagttgccg accggctcgc cgacccggcc cggtcggtcg
     2401 acatcctcgt caacaacgcc ggattcggcc tcaagggcgg cttcctcgac aacgatctcg
     2461 cgcaggagga agagctcttc gacgtgctct gccgagcggt gctggtcctc tcgcacacgg
     2521 cggcccgggc catgcgcgag cggggcagcg gccggatcat caacgtctcc tccgtcgcga
     2581 gtttcattgc ggggagcagc tattcggcgg caaagtccta tgtcaccgtg ctcacggagg
     2641 cgctgtcggt cgctctcgca ggctcgggag tgacggcgac ggcgctgtgc ccgggattcg
     2701 tgcacacaga gttccacgag cgcgccgaga tggggatgga ctatctgccg gatatggcct
     2761 gggtgccggt ggacaagctc gttcgtgagg cactcctcga ctccgagaag ggcaaggtca
     2821 tctcggtgcc cgggtggcag tacaaggccg ccacccgcat cctcaagctc accccgcgag
     2881 ccctggcccg ccacccccgc atcgtcggcc agcaccgcaa accctgaccc cgtcgagatg
     2941 tcgattcggc cccatcgaga tgtcactgca tagggtgagc ggtatgacgg acattgccgc
     3001 cgaccgggcc gcgctactgg agcacatcaa ggccaaggcc atcgtgcacg gacgagtgac
     3061 gctgtcctcg ggcaaggagg ccgactacta cgtcgacctg cgccgcatca ccctcgacgg
     3121 ggaggcggct ccattggtgg gccgggtgat gcgcgagctg gtcaaggacc tggacttcga
     3181 cgctgtcggc gggctcacgc tcggcgccga cccggtcgcc acctcgatgc tgcacgccgc
     3241 ggcggcggct gggcagcggc tcgacgcctt cgtcgtccgc aagtccggca aagcccatgg
     3301 gctgcagcga cgcatcgaag gaccgtccat cgccggccgc cgggtg
//
LOCUS       DAOPJG010000274         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_32291_length_3317_cov_6.030043, whole genome shotgun sequence.
ACCESSION   DAOPJG010000274 DAOPJG010000000
VERSION     DAOPJG010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32291_length_3317_cov_6.030043"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKU75_16225"
     CDS             <1..283
                     /locus_tag="PKU75_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82474.1"
                     /translation="TGIAEHIQATAALGVDVFAASLADAPEDMPVQRARAARIARTCQ
                     AHVAFASFAGPTGRGYEQTAGGSAIYSPAGEVLADCGSDSGEIARTGIG"
     gene            complement(378..956)
                     /locus_tag="PKU75_16230"
     CDS             complement(378..956)
                     /locus_tag="PKU75_16230"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPF82475.1"
                     /translation="MADVVLYAFDTMADWEYAYITTVLQMQARGGDDRYRLVVAGATG
                     EPVTTLGGLRVLPERALTEVAKDDIALLLLPGGNTWLDGPHQQVLDLARRLLDARRPV
                     AGICGATRAMARAGLLERHAHVVDADADEPDYPGSASRVHASVAHDGPLVTAIGEAPL
                     EFSRAVFTLLDLDDAAGIDAWYASFKSVPDAR"
     gene            1019..3154
                     /gene="ligA"
                     /locus_tag="PKU75_16235"
     CDS             1019..3154
                     /gene="ligA"
                     /locus_tag="PKU75_16235"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPF82476.1"
                     /translation="MTNQPDLAPDVVPDDVRHEWTELAEQATAAQFAYHVKDAPTISD
                     GEYDALIRRLQQLEEAHPSLRTPESPTQTVGGAVFSTDFVAVDHLERMLSLDNVFSPE
                     EFAAWAARVEREAGGAAYHLLCELKIDGLAVNLLYEKGRLTRALTRGDGRTGEDVTLN
                     VKTIDGIPSVLRGADVPELVEIRGEVFFPIEAFADLNASLVAAGKAPFANPRNAAAGS
                     LRMKDPRVTATRPLHMLVHGIGARRGFDIDRQSAAYDLLEAWGLPVSSHYRVLDTPEQ
                     AQGFIAGFGERRHSVEHEIDGVVVKVDEIAVQRQLGATSRAPRWAIAYKYPPEEVNTK
                     LLDILVNVGRTGRVTPYGVMEPVTVAGSTVTNATLHNASEVERKGILIGDTVVLRKAG
                     DVIPEILGPVVDLRDGSERAFVMPTHCPSCGAELAPEKEGDKDIRCPNARSCPSQLRE
                     RLFGLAGRGAFDIEALGWEGATSLLDSGVLADPQLGGPSESMLFSLGEKEIARVPLYT
                     RAAKKTDPPEAVVDGRVLSANGQRLVANLHSAKEQPLWRVLVALSIRHVGPTAARALA
                     QHFGSMAAIRDADAEALSGVEGVGGVIADAVRDWFDREGNDWHVRIVEQWAADGVRMV
                     DERDESVEQTLAGMTVVVTGSLEGFSRDSAKEAILARGGKAAGSVSKNTDWVVVGPGA
                     GSKEAKARELGLTILDEAGFVRLLETGSA"
BASE COUNT          521 a         1092 c         1231 g          473 t
ORIGIN      
        1 cacgggcata gcggagcaca tccaagccac tgctgcgctg ggggtggacg tgttcgcggc
       61 gtcactggct gatgcgccag aggacatgcc tgtgcaaagg gcacgtgctg cgcgcatcgc
      121 ccgcacctgt caggcgcacg tcgccttcgc cagtttcgcg gggcctaccg ggcgtggtta
      181 tgagcagacg gccggcggct cggccatcta ctccccggcc ggggaagtgc tggcggattg
      241 tggctcggat agtggtgaga tcgccagaac aggcattggc tgatcccctg ctaggtccag
      301 ggcgggtcgc tcatgccgcg aagcgcgcgt cgtgagcgag acgtcgcatg gaattgggag
      361 acacgcccta gcgggggcta gcgggcgtcc gggacggact tgaaggaggc gtaccaggcg
      421 tcgatgccgg cggcgtcgtc cagatcgagg agggtgaaga cggcgcggct gaactcgagc
      481 ggggcctcgc cgatcgcggt gacgaggggg ccgtcgtggg cgacggaggc gtgcacccgc
      541 gaggcgctgc ccgggtagtc gggctcgtcc gcgtcggcgt cgacgacgtg ggcgtgccgc
      601 tccaacaggc ccgcgcgggc catcgcgcgc gtcgccccgc agatgccggc gacggggcgc
      661 ctggcgtcga gcaggcggcg ggccagatcg agtacctgct ggtgagggcc gtcgagccag
      721 gtgttcccgc cagggagcag tagcagcgcg atgtcgtcct tggcgacctc ggtcagggcg
      781 cgttcgggca gcacgcgcag gccgcccagg gtggtgaccg gttcgccggt ggcaccggcg
      841 acgacgagcc ggtagcggtc gtcaccgccg cgcgcctgca tctgcaggac ggtggtgata
      901 taggcgtact cccagtccgc catggtgtcg aaggcataga ggacgacatc ggccatgccc
      961 tcactctagg cccgggggaa acccggccgc tgtcggtggc ctgaggcagg atgtccgcgt
     1021 gaccaaccag ccggacctgg cccccgatgt cgtgcccgac gacgtgcgcc acgagtggac
     1081 cgaactcgcc gagcaggcaa ccgcggcgca gttcgcctat cacgtcaagg acgcgccgac
     1141 gatcagcgac ggggagtatg acgcgctgat ccggcggctg cagcagctcg aggaggccca
     1201 tccttcgctg cgcacgcccg agagcccgac ccagacggtc ggcggggcgg tcttcagcac
     1261 cgacttcgtc gcggtcgacc acctggagcg gatgctcagc ctcgacaacg tcttctcgcc
     1321 ggaggagttc gccgcctggg cggcgcgggt ggagcgagag gcggggggcg cggcatacca
     1381 cctgctctgt gagctgaaga tcgacgggct cgccgtcaac ctcctctacg aaaagggccg
     1441 gctgacccgt gccctcaccc gcggcgacgg ccgcaccggg gaggacgtga cgctgaacgt
     1501 caagacgatc gacggcatac ccagcgtcct gcggggagcg gacgtgcccg agctggtcga
     1561 gatccgcgga gaggtgttct tcccgatcga ggccttcgcc gatctcaacg cgagcctcgt
     1621 cgcggccggc aaggcaccct tcgccaaccc gcgcaacgct gccgcggggt cgctgcggat
     1681 gaaggatccc cgggtgaccg ccacccggcc gctgcacatg ctcgtccacg gcatcggcgc
     1741 ccggcggggg ttcgacatcg accggcagtc cgcggcatac gacctgctcg aggcgtgggg
     1801 gctgccggtg agcagccact atcgggtgct cgacacaccc gaacaggcgc aggggttcat
     1861 cgccgggttc ggtgagcggc ggcacagcgt cgagcacgag atcgacggcg tggtcgtcaa
     1921 agtcgacgag atcgccgtgc aacgtcagct gggcgcgacg agccgggcgc cgcggtgggc
     1981 gatcgcctac aagtaccctc ccgaggaggt caacaccaag ctcctcgaca tcctggtcaa
     2041 cgtcggccgc accgggcgcg tgacaccgta tggcgtgatg gagccggtca cggtcgcggg
     2101 ctcgaccgtc accaatgcca ccctgcacaa cgcctcagag gtcgagcgca aggggatcct
     2161 catcggcgac acggtcgtgt tgcgcaaggc cggcgacgtc attcccgaga tcctcgggcc
     2221 ggtggtcgac ctgcgtgacg gatccgaacg cgctttcgtc atgccgacgc actgcccttc
     2281 gtgcggggcc gaactcgcgc cggagaagga gggcgacaag gacatccgct gtcccaacgc
     2341 ccgttcgtgt ccgagccagc tgagggagcg gttgttcggg ctggccgggc ggggtgcctt
     2401 cgacatcgag gcgctcggct gggagggcgc tacgagcctg ctcgactcgg gtgtgctggc
     2461 ggacccgcag cttggcgggc ccagcgagtc gatgctgttc tcgttggggg agaaggagat
     2521 cgcccgtgtg cccctctata cgcgggcggc caagaagacc gatccaccag aggcggtcgt
     2581 cgacggtcgc gtgctgtcgg ccaatggcca gcgcctcgtc gccaacctgc actcggccaa
     2641 ggagcagccg ctctggcggg tgcttgtcgc gctgtccatc cggcacgtcg ggccgacggc
     2701 cgcccgggcg ctggcgcagc acttcgggtc catggcggca atccgggacg ccgatgccga
     2761 ggccctgtcg ggcgtcgagg gtgtcggcgg cgtcatcgcc gacgcggtcc gcgattggtt
     2821 tgacagggag ggcaacgact ggcacgtgcg gatcgtcgag cagtgggcgg ccgacggggt
     2881 gcggatggtc gacgagcgcg acgaatccgt cgagcagacg ctggccggca tgacagtcgt
     2941 cgtcaccggg tccctcgagg ggttcagccg cgactcggcc aaggaggcga tcctcgcccg
     3001 cggcggcaag gcggccggct cggtgtcgaa gaacaccgac tgggttgtcg tcggcccggg
     3061 cgccggctcc aaggaggcca aggcgcgcga actcgggctg acgatcctcg acgaggccgg
     3121 cttcgtgcgg ctcctcgaaa ccgggtccgc ctgagcgtcg gaccgcccac gtgaaggcaa
     3181 cgccggaccc tcgctcgaag gtccggcgcc gccggtgagt gtgtacggct gcgctcaggc
     3241 gcgcgccatg atccacatga gaagggcgat gatcagcagc acgccgacga tcgtccagat
     3301 cagtcctgaa ccacgca
//
LOCUS       DAOPJG010000275         3295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_32579_length_3295_cov_7.231790, whole genome shotgun sequence.
ACCESSION   DAOPJG010000275 DAOPJG010000000
VERSION     DAOPJG010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32579_length_3295_cov_7.231790"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PKU75_16240"
     CDS             complement(<1..1151)
                     /locus_tag="PKU75_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPF82477.1"
                     /translation="MAASRTAPQSTAKTASGRLSFAKIRETLEVPDLLALQTESFDWL
                     LGNERWQERVADALDAGRKDVPDRSGLEEIFEEISPIEDFSGSMSLSFRDHRFEESKY
                     SIEDCKERDQTYSAPLFVTAEFMNTTTGEIKSQTVFMGDFPLMTERGTFIINGTERVV
                     VSQLVRSPGVYFERTPDKTSDKDIYTARVIPSRGAWLEFEIDKRDMIGVRVDRKRKQS
                     VTVLLKALGMSEAEIREEFAGFESMELTLEKDHIAGQDDALLDIYRKLRPGEPPTREA
                     AQTLLDNLYFNPKRYDLAKVGRYKIGRKLGRDVDLHDSVLTLEDIVATIKYLVALHRG
                     DETITGVRDGETVELAVETDDIDHFGNRRLRSVGELIQNQVRTGLSRMER"
     gene            complement(1584..1979)
                     /gene="rplL"
                     /locus_tag="PKU75_16245"
     CDS             complement(1584..1979)
                     /gene="rplL"
                     /locus_tag="PKU75_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPF82478.1"
                     /translation="MAKLSTDELLEAFKEMTLIELSEFVKQFEDTFGVTAAAPVAAPA
                     AGPAAGGAGGEAAAAEEKDEFDVILAAAGDKKIQVIKEVRSLTSLGLKEAKDLVDGAP
                     KPVLEKVDKATADKAKEALEGAGATVELK"
     gene            complement(2053..2700)
                     /gene="rplJ"
                     /locus_tag="PKU75_16250"
     CDS             complement(2053..2700)
                     /gene="rplJ"
                     /locus_tag="PKU75_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPF82479.1"
                     /translation="MAKTDKSAAIAELTEAFRNSNAAVLTEYRGLTVKQLTELRTNLR
                     GNATYAVVKNTLTDLAAKEAGVTAFEGQLAGPSAIVFVDGDAVEVAKSLRDFAKAHPL
                     LVIKGGVLDGRALTAAEITKLADLESREVLLAKLAGAMNASLSRAVYLFAAPLSQAAR
                     TVDALRAKVEAEGPAAPAAPQDPVEAPADSPQDAPADAPQDAPAADVAEGGDDNA"
     gene            complement(3036..>3295)
                     /locus_tag="PKU75_16255"
     CDS             complement(3036..>3295)
                     /locus_tag="PKU75_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82480.1"
                     /translation="VTAKDGDTTTYSLELDLKKLFEAMDTELPTTMTLPETIPVTYVL
                     DGENRPVKSTINMGTNIVVEYSDWGKKVDIKAPPASEVTTG"
BASE COUNT          487 a         1033 c         1185 g          590 t
ORIGIN      
        1 cgctccatcc gggacaggcc ggtgcggacc tggttctgga tgagctcgcc gacgctgcgc
       61 aggcggcggt tgccgaagtg gtcgatgtcg tcggtctcga cggccagctc gaccgtctcg
      121 ccgtcacgga caccggtgat cgtctcgtcg ccgcggtgca gggccacgag gtacttgatc
      181 gtcgcgacga tgtcctcgag ggtgaggacg gagtcgtgca ggtcgacgtc gcggccgagc
      241 tttcgcccga tcttgtaacg gccgaccttg gccaggtcgt agcgcttggg gttgaagtag
      301 aggttgtcca gcagggtctg ggcggcctcg cgggtcggcg gctcgcccgg gcgcagtttg
      361 cggtagatgt cgagcagagc gtcgtcctgg ccggcgatgt ggtccttctc gagcgtcagc
      421 tccatggact cgaatccggc gaactcctcg cggatctcgg cctcggacat gccgagcgcc
      481 ttgagcagca cggtgaccga ctgcttgcgc ttgcggtcga cgcgcacgcc gatcatgtcg
      541 cgcttgtcga tctcgaactc cagccacgcg ccgcggctcg ggatgacccg ggcggtgtag
      601 atgtccttgt cggacgtctt gtcgggggtg cgctcgaagt agacgcccgg ggagcggacg
      661 agctgggaga cgaccacgcg ctcggtgccg ttgatgatga aggtgccgcg ctcggtcatc
      721 agggggaagt cgcccatgaa gaccgtctgg ctcttgatct caccggtggt ggtgttcatg
      781 aactccgcgg tgacgaacag cggcgcggag taggtctggt cccgctcctt gcagtcctcg
      841 atggagtatt tggactcctc gaagcggtga tcgcggaagc tgaggctcat cgagccggag
      901 aagtcctcga tcggggagat ctcctcgaag atctcctcga ggcccgaacg gtccggcaca
      961 tccttgcggc cggcgtcgag ggcgtcggcg acgcgctcct gccagcgctc gttgcccagc
     1021 agccagtcga aggactcggt ctggagggcg aggaggtcgg ggacttcgag ggtttcgcga
     1081 atcttcgcga acgacaaacg gccggaagcc gtctttgcag ttgactgagg tgcggtgcgc
     1141 gaggcagcca aggaggggtc cttccacagg cctgtctggg tccgctatcg gcccgcgcac
     1201 accacggcgc acgttcagga ctccgagcgt tcggcccggg acatacctgt agcacgtcga
     1261 ggagagtgtg ggaagacagc gcaaagaagc actatacggc atttaggcat ggctgtccaa
     1321 gcctgtccag tccacgtggc cctggcggcg gtcgcactgc gacgcaaaga ggatggcgct
     1381 aagacgccgc ccacgtcaag cgtagcggtc acacggcgcg tcgccgaggg tcgtatgccg
     1441 tcgccccccg gacgccgcac ctgcggcgag cgggcgacat acgagaacga aaacccctcc
     1501 cccgcaacgg ttttcgttgc ggggaagggg tgatcggtag ggggcccgga gaccggctgg
     1561 tgagcctgtc tacagggtca caatcacttc agttcgacgg tggcgccggc gccctcgagg
     1621 gcttccttcg ccttgtcggc ggtggccttg tcgaccttct cgaggacggg cttgggggcg
     1681 ccgtcgacga ggtccttggc ctccttcagg ccgagggagg tcagcgagcg gacctccttg
     1741 atgacctgga tcttcttgtc gccggcagcg gcgaggatga cgtcgaactc gtccttctcc
     1801 tccgcggcgg cggcctcgcc accggcacca ccggcggccg ggccggcggc cggggcggcg
     1861 accggggcgg cggcggtgac gccgaaggtg tcctcgaact gcttgacgaa ctcggagagc
     1921 tcgatcaggg tcatctcctt gaatgcctca aggagctcgt cggtgctgag cttcgccatg
     1981 atgggcgtcc tttctcgtta cttctcgtat gccgtgagtg gcggtttggg tgcctgcgtc
     2041 cgcgggtgcg ggtcaggcgt tgtcgtcgcc accctcggcg acgtcggcgg ccggggcgtc
     2101 ctgaggggcg tccgcaggag cgtcctgcgg ggaatccgcc ggggcctcga ccgggtcctg
     2161 gggagctgcc ggggcagcgg ggccctccgc ctcgaccttg gcgcgcaggg cgtcgacggt
     2221 gcgagcggcc tgcgacaagg gcgcggcgaa gaggtagaca gcgcggctca gcgaggcatt
     2281 catggccccg gcaagcttgg cgagcagaac ctcgcgcgac tcgaggtcgg cgagcttggt
     2341 gatctcggcg gcggtcaggg cgcgcccgtc gaggacacca cccttgatca ccagcagagg
     2401 gtgagccttg gcaaagtcac gcagcgactt ggcaacctcg acggcgtcgc cgtcgacgaa
     2461 gacgatggcg ctggggccgg cgagctgacc ctcgaaagcg gtcaccccgg cttccttggc
     2521 ggccaggtcg gtcagcgtgt tcttgaccac ggcgtaggtg gcgttgccac ggaggttggt
     2581 gcgcaactcg gtgagctgct tcacggtcaa gccgcggtac tccgtgagaa cggccgcgtt
     2641 cgagttgcgg aatgcctccg tcaactcggc aatggcagcc gacttgtcgg tcttcgccat
     2701 gtgggcctcc ttccgtcgtc tcgaccgacg ggtgggcccg aaacgcgaaa acgccccggc
     2761 gcagggcacc gaggcgagca cacgtcatac cgtgtgaagc tgtcgtcctg cgctggtcgc
     2821 ccgcggtgcg ggaccttcga ctggcggcgg tatgccgtca gcgaccggcg gtcattggtc
     2881 ccgtcaaggt tacgtccttc cccccggaac ccccaaatcg cgggggccgc aggatccccg
     2941 cccgatgccg aaaaccccga gtgcgggcca tgagcgtcat ggcccgcact cggggttcgt
     3001 ctccgaccgg cgccgggatc agccggcgcc cgggatcagc cggtggtgac ctcggaggcc
     3061 ggcggggcct tgatgtcgac cttcttgccc cagtcggagt actcgacgac gatattggtg
     3121 cccatgttga tcgtggactt gaccgggcgg ttctcgccgt ccaggacgta ggtgaccggg
     3181 atggtctccg gcagcgtcat ggtcgtcggc agttcggtgt ccatcgcctc gaagagcttc
     3241 ttcaggtcca gttcgaggct gtatgtcgtg gtgtcgccgt ccttggccgt cacct
//
LOCUS       DAOPJG010000276         3232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_33451_length_3232_cov_8.576959, whole genome shotgun sequence.
ACCESSION   DAOPJG010000276 DAOPJG010000000
VERSION     DAOPJG010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33451_length_3232_cov_8.576959"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKU75_16260"
     CDS             complement(<1..516)
                     /locus_tag="PKU75_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPF82481.1"
                     /translation="MMPTLGPDWMDPAWLLDRFGSQFFWVSCAIVFVECGLLFPILPG
                     DSLLFAVGLFIANGALKFNIVTACVILTIAAFAGNVVGYEIGRAIGQPLYERQGRFVN
                     KANFDKTHDFFEKYGAKALVLGRFVPIVRTFITVVAGVGKMDRRHFFVWSAVGAALWA
                     THAAGAAFGATA"
     gene            complement(743..1864)
                     /locus_tag="PKU75_16265"
     CDS             complement(743..1864)
                     /locus_tag="PKU75_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPF82482.1"
                     /translation="MSPDTLNSMLAGVQAVTWQSLVMLTVGCLLIALAVVKEYEPLLL
                     LPIGAGCILANLPLSPLADEGGMLDILYNIGVGNELFPLLIFIGIGAMTDFGPLLENP
                     RMVLLGAAGQLGIFLTLILALALGFSRPEAASIGVIGAIDGPTSIYVSGILAPDLLGP
                     ITVAAYSYMSLVPIIMPPIMRLLTTKRERAIRMPYSQRVISKRTRVLFPVVVTIVVGI
                     LVPFATPLIGMLMLGNLLRESGVVERLTQAASNELANVVTLFLGLAIGSTMVGEDFIK
                     ASTLFILGLGLIAFCLDTAAGVMFGKLLNLFSGGKFNPLIGAAGISAFPMAARVVQQE
                     AQREDYDNFLLMHAMGANAAGQVASVVAGGVLLAFMGAG"
     gene            complement(1861..2403)
                     /locus_tag="PKU75_16270"
     CDS             complement(1861..2403)
                     /locus_tag="PKU75_16270"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82483.1"
                     /translation="MRRYTVTVGDQAYVIDVEERSADTFTVILDGRPLSVRVDGHEDL
                     AQAAIRPQLEIADSPGAAGVPTAAGAAGAASAAGHGTAAARPTGATTPHTPPPSRAPR
                     AAAGTDATTLTAPMPGVILTIVAPVGTVVAQGDPLLVLEAMKMKNTLRAGRDGRVAEV
                     AVGVGDQVKFGDVLVRVEDA"
     gene            complement(2408..2806)
                     /locus_tag="PKU75_16275"
     CDS             complement(2408..2806)
                     /locus_tag="PKU75_16275"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPF82484.1"
                     /translation="MRDIGFGLGMTAAGMGTVFAMLLLLMIIVSLMGRMDRQPSSAEN
                     AGRDGLAGEDLDAMSGIDDAPDTAPRVLADGLTEDQIAAVSVAVLLHAEIRRQQAAPA
                     MRTHQPGSHIFASRWVANGRGLQTQPWRRS"
     gene            complement(2812..>3232)
                     /locus_tag="PKU75_16280"
     CDS             complement(2812..>3232)
                     /locus_tag="PKU75_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPF82485.1"
                     /translation="IRHGAKIIYAYCQATVPKVSVVTRKAIGGSYVAMSSKQMNNDIA
                     FAWPSAEIAVMGPEGAARLLNRQALREAEDPASLEAQFVTDYREKFFNPYRAADLGQI
                     DEVIEPRETRPHLIRSFEVLQTKVQGGLPKKHGLFPV"
BASE COUNT          540 a         1098 c         1106 g          488 t
ORIGIN      
        1 ggcggtcgcc ccgaaggcgg ccccggcggc atgggtggcc cacagggcgg cgccgaccgc
       61 actccatacg aagaagtggc gacgatccat cttgcccacg ccggcgacga cggtgatgaa
      121 ggtgcgcacg atggggacga agcggccgag caccagcgcc ttcgcgccgt acttctcgaa
      181 gaagtcgtgg gtcttgtcga agttggcctt gttgacgaac cgcccctggc gctcatacag
      241 cggctgaccg atcgcccggc cgatctcgta gccgacgaca ttgccggcga acgcggcgat
      301 cgtcaggatc acgcaggcgg tgacgatatt gaacttcagc gcgccattgg cgatgaacag
      361 ccccaccgcg aagagcagcg agtcgcccgg caggatcggg aagagcaggc cgcactcgac
      421 gaacacgatc gcgcagctga cccagaagaa ctggctgccg aatcgatcga gcagccaggc
      481 ggggtccatc cagtcggggc caagggtggg catcatcacg cgtcaagggt aggtgaccaa
      541 ccctgtgagg cagctgtatg ccgcccctgc ccggcctgtc gcatcacctg tcgcatcacc
      601 ggccgagtcg ccgtcgccgg cccactgacc ccaaccccct cggataacac caaccccctg
      661 ttctagcgcg aactccggga catatatccg cggcagttgg cgccttctcc gggagttcgc
      721 gcattttgac gcagttggcc gttcagccgg cgcccatgaa ggcaagcagc acgccgccgg
      781 cgaccacgga ggccacctgg cctgccgcat tggcgcccat ggcgtgcatg aggaggaagt
      841 tgtcgtagtc ctcccgctgc gcctcctgct gcaccacgcg ggcggccatt gggaaggcgg
      901 agatgccggc cgctccgatc agcgggttga acttgccccc gctgaacagg ttgagcagct
      961 tgccgaacat caccccggcg gcggtgtcga gacagaaggc gatcaggccg agcccgagga
     1021 tgaacagcgt cgaggccttg atgaagtcct ccccgaccat cgtcgagccg atggccagac
     1081 cgaggaagag ggtgacgacg ttcgccagct cgttggaggc cgcctgcgtc agccgctcga
     1141 cgactcccga ctcgcgcaac aggttgccga gcatgagcat gccgatgagc ggcgtggcga
     1201 acggcacgag gatgccgacg acgatcgtca cgacgaccgg gaagagcacg cgggtgcgct
     1261 tgctgatcac gcgctgggag tagggcatcc ggatcgcgcg ctctcgcttc gtggtgagca
     1321 ggcgcatgat cggcggcatg atgatcggca cgagcgacat atagctgtat gccgcgacgg
     1381 tgatcggccc gagcaggtcc ggcgccagga tgccgctgac atagatcgac gtcggcccgt
     1441 cgatggcgcc gatgactccg atggacgccg cctccggccg gctgaaaccc agtgcgagag
     1501 cgaggatcag cgtcaagaag atgccgagct ggcctgcggc gccgagcagc accatacggg
     1561 ggttctccag cagcggaccg aagtccgtca tcgcgccgat gccgatgaag atcagcagcg
     1621 ggaagagttc gttgccgacg ccgatgttgt agaggatgtc cagcatcccg ccctcgtcgg
     1681 ccagcggcga gagcggcagg ttcgccagga tgcagccggc gccgatcggc agcagcagca
     1741 acggctcgta ctccttgacc acggccagcg cgatgaggag gcacccgacg gtgagcatga
     1801 cgagcgactg ccacgtgacc gcctgcaccc cggcgagcat ggagttgagg gtgtcgggac
     1861 tcatgcgtcc tcgacccgca ccagcacgtc gccgaacttc acctggtcac ccaccccgac
     1921 cgcgacctcc gcgacacgcc cgtcccggcc ggcgcgcagc gtgttcttca tcttcatcgc
     1981 ctcgagcacg agcagcggat cgccctgggc gacaacggtt ccgacgggtg ccacgatggt
     2041 gaggatgacg ccgggcatgg gcgcggtcag cgtggtcgcg tcggtgccgg ccgcggcgcg
     2101 aggggcccgc gacgggggcg gggtatgcgg tgtggtcgcc cccgtcgggc gcgctgcggc
     2161 cgtcccgtgg ccggcagcgc tcgccgcccc cgccgccccc gccgccgtcg gcacccccgc
     2221 cgcgcccggg gagtcggcga tctccagttg cggacggatg gcggcctggg cgaggtcctc
     2281 gtgcccgtcg acacggaccg acagcggccg cccgtcgagg atcacggtga aggtgtccgc
     2341 gctccgctcc tcgacgtcga tgacataggc ctggtcaccg acggtcaccg tgtatctgcg
     2401 catgccgtca gctcctacgc caaggctggg tctgtagccc gcggccgttg gcgacccaac
     2461 ggctcgcgaa gatgtggctt ccgggttggt gggtgcgcat cgccggggcc gcctgctggc
     2521 ggcgaatctc ggcgtgcagg agcacggcga ccgagacggc ggcgatctgg tcctccgtca
     2581 ggccgtcggc aaggacccgc ggtgcggtgt ccggggcgtc gtcgatgccg ctcatcgcgt
     2641 cgaggtcctc gccggccagc ccgtcgcgtc cggcgttctc ggccgaggag ggttgacggt
     2701 ccattcgtcc catgagcgag acgatgatca tgagcaggag cagcatggcg aagacggtgc
     2761 ccatgccggc cgccgtcatg ccgagtccga aaccgatgtc tctcatggca atcagaccgg
     2821 gaagagtccg tgcttcttcg gcagcccgcc ttgcaccttg gtctgcagca cctcgaagga
     2881 gcggatcagg tgcgggcggg tctcgcgcgg ctcgatgacc tcgtcgatct gccccaggtc
     2941 cgcggcgcga tagggattga agaacttctc gcgatagtcg gtgacgaact gcgcctccag
     3001 gctcgccgga tcctcggcct ccctgagcgc ctgccggttc agcagccgcg ccgccccctc
     3061 cggacccatg acggcgatct ccgcgctcgg ccaggcgaac gcgatgtcgt tgttcatctg
     3121 cttcgaactc atcgcgacat acgacccgcc gatcgccttg cgcgtgacca ccgacacctt
     3181 gggcaccgtc gcctggcagt aggcgtagat gatcttcgcg ccgtgccgga tc
//
LOCUS       DAOPJG010000277         3189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_34058_length_3189_cov_7.401085, whole genome shotgun sequence.
ACCESSION   DAOPJG010000277 DAOPJG010000000
VERSION     DAOPJG010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34058_length_3189_cov_7.401085"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PKU75_16285"
     CDS             complement(<1..1223)
                     /locus_tag="PKU75_16285"
                     /inference="COORDINATES: protein motif:HMM:NF018259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HPF82486.1"
                     /translation="MTVALPVSIAPQDGESIESWLEHLADANGLTTAQLLTATGRGRA
                     GTRYLTLAPSPDTVARLAALARVGEQAVYAATLASFDGTALDLTGLDPTDRLSYRQVA
                     ARGWVPAHGTQICPTCLAETGAWRTTWRLLIVTACTRHRTLLVARCPACRRPFRDQRH
                     SHLRRVGGSLVCGNPLGDGPTRQCQHDLTTTATTPADEAVMLTQRRVDAALDGHDIEV
                     LGQPACPAAYLTDLRHLTTLMLHLAGQPGATDLAHWPATLAHEAEQRTGDRGPRWGLR
                     PPDDPGVRGAALATADAILAAPDVDAAAAALTPWTELPPTTPDGPLGWLADHTVMTPT
                     LTRLVMAARAPHRRLSHHLDTHTGGRMPINLLVIPQVIPHPHYLEHLQGAFDSSEDTV
                     RAFASLSLARLHPDVT"
     gene            complement(1220..2422)
                     /locus_tag="PKU75_16290"
     CDS             complement(1220..2422)
                     /locus_tag="PKU75_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82487.1"
                     /translation="MSIWHVYPTYIRLVDGSHIPLVLMFQTDTRELVGGTVADTHEKV
                     SSWIRDLTAAHGVPEGVIHVDDSSFPGLTDAITVGLAHRGYQLGTELQPNVNDLVWVI
                     DEGRRSIAAINAARPASAIHITHALDGLLTTFAQTHETVVAIRRLDAGAGPMKEAAAG
                     LPPVAVVLDIDGVIAPIGASTQWVDDTMIGDDEGGVHLSPLMCAALDDLHRPGQVGCY
                     WLTDWTHTMRHHLDLLPGRSWAEITAPADTPARPVRPEDRWNPAPWWKQRALDDFLRR
                     HPEIRHVVWIDARLRPDHASPAVGVLEPCAMWTGTGRLANCTNVLLVAPDMRAGLTSD
                     DLSSLADWIATHTATASNASRRRGAEHPRHHLIYGDHPSEELQSHLHDFLRDARAPRS
                     RTPPAGPS"
     gene            complement(2419..2727)
                     /locus_tag="PKU75_16295"
     CDS             complement(2419..2727)
                     /locus_tag="PKU75_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82488.1"
                     /translation="MRGISREVLARDAAWSLADGACKDLTIEWSRWENTFAAALTQAG
                     RLAALAAPAAVCSACPITAECADLAELSGYTGIAAGEGYRNGRLDLARQRHPRQRRTA
                     "
     gene            complement(2727..3104)
                     /locus_tag="PKU75_16300"
     CDS             complement(2727..3104)
                     /locus_tag="PKU75_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82489.1"
                     /translation="MRPIDNPALENLHQRTRAMLTAIDDTPLERIDHGHYQHYYEELR
                     HLVEEWITATSAKPLALPKRTWVITRHGVVVGVCTTDTVTALTAGIPDLTVTPITLCS
                     SAIEDLRAGWSTDPPPARRARER"
BASE COUNT          418 a          924 c         1331 g          516 t
ORIGIN      
        1 gtgacgtccg ggtgcagacg agcaagcgac agggatgcga aggcccggac ggtgtcctcg
       61 ctggagtcga acgcgccttg caggtgttcg agatagtggg ggtgcgggat gacctggggg
      121 atgacgagca ggttgatcgg catacggcct ccggtgtggg tgtcgaggtg gtgggacagg
      181 cgccggtgcg gggcgcgggc cgccatgacc agccgggtga gggtgggggt catgacggtg
      241 tggtcggcga gccagccgag gggcccgtcc ggcgtggtcg gggggagctc ggtccacggc
      301 gtcaacgcgg ccgcggcggc gtcgacgtcg ggggcggcca ggatcgcgtc ggcggtggcc
      361 agggcggcac cgcggacacc ggggtcgtcg ggtggtcgga ggccccaccg tgggccgcgg
      421 tcgccggtcc gctgttctgc ctcgtgggcg agggttgcgg gccagtgggc gaggtcggtg
      481 gcgccgggtt ggccggcgag gtgaagcatc agggtggtca ggtgccgtag gtcggtcagg
      541 taggccgcgg ggcaggcggg ctgtccgagg acctcgatgt cgtggccgtc gagggctgcg
      601 tcgacccgcc gctgcgtcaa catgacagcc tcgtctgccg gggtggtcgc ggtggtggtg
      661 aggtcgtgtt ggcactggcg ggtgggtccg tcgccgagcg ggttcccgca caccaggctc
      721 cccccgacgc ggcggaggtg cgaatgccgt tggtcccgga acgggcggcg gcaggccggg
      781 catcgggcga ccaggagggt gcggtggcgg gtgcaggcgg tgacgatgag caggcgccag
      841 gtggtgcgcc aggccccggt ctccgcgaga caggtcgggc agatctgggt gccatgggcg
      901 gggacccagc cacgggcggc gacctgccgg taggagagcc ggtcggttgg gtcgagcccg
      961 gtgaggtcga gggcggtgcc gtcgaacgag gcgagggtcg cggcatacac ggcctgctcg
     1021 ccgacgcggg cgagggcagc gagtcgggcg acggtgtccg gggagggggc gagggtgagg
     1081 tagcgggtgc cggctcggcc ccggccggta gcggtgagga gttgggcggt ggtcaggccg
     1141 ttggcgtcgg cgaggtgctc cagccaggac tcgatcgact ccccgtcctg aggggcgatc
     1201 gacaccggca gcgcgacggt catgacgggc cggctggtgg tgttcgcgac ctcggggccc
     1261 gggcgtcgcg gaggaagtcg tggaggtgac tttgcagttc ctcgctgggg tggtcgccat
     1321 agatcaggtg atggcgcggg tgctccgccc cgcgccgtcg tgatgcgttg ctggctgtcg
     1381 cggtgtgggt cgcgatccag tcagccaggc tggacaggtc gtcggaggtc aggccggcgc
     1441 gcatgtcggg cgccacgagc agcacgttcg tgcagttggc gagccggccg gtgccggtcc
     1501 acatcgcgca cggctccagg acacctacag cgggtgaggc gtggtcgggg cggagcctgg
     1561 cgtcgatcca cacgacgtgt cggatctcgg ggtgccgtcg tagaaagtcg tcaagggctc
     1621 gctgcttcca ccaaggggcg gggttccacc ggtcctcggg ccgaaccggg cgcgccgggg
     1681 tgtcggcggg ggccgtgatc tcggcccagg accggccggg cagcaggtcg aggtggtggc
     1741 gcattgtgtg ggtccagtcg gtgagccagt agcagccgac ctggcccggg cggtgcagat
     1801 cgtccagggc ggcacacatc aacggcgaca ggtgcacacc gccctcatca tcgccgatca
     1861 tcgtgtcgtc gacccactgg gtcgaggcgc cgatcggtgc gatcaccccg tcgatgtcga
     1921 ggacgaccgc gaccggcggc agcccggccg ctgcttcctt catcgggccc gccccggcgt
     1981 caagcctccg gatggccacg acggtttcgt gcgtttgtgc gaaggtggtc aacagtccgt
     2041 caagggcgtg ggtgatgtga atcgcggacg cgggacgggc ggcattgata gcggcaatgg
     2101 agcggcggcc ttcgtcgatg acccacacga gatcgttgac gttgggttga agctcggtgc
     2161 cgagctggta acccctatgg gcgaggccga cggtgatggc gtcggtcagg ccggggaagc
     2221 tcgagtcgtc gacgtggatc accccttccg gcacaccatg cgctgccgtg agatctcgga
     2281 tccaagagga caccttctcg tgggtgtcgg cgacggtgcc gccgaccagc tcccgggtgt
     2341 cggtttggaa catcaggacg agcgggatgt gggagccgtc gaccaggcgg atatacgtgg
     2401 ggtagacgtg ccagatgctc atgcggtcct ccgctgtctt gggtgtcgtt ggcgggcgag
     2461 gtcgaggcgg ccattgcggt agccctcgcc ggcggcgatg ccggtgtaac cggacagttc
     2521 ggcgaggtcg gcgcactctg cggtgatggg gcaggcggag cagacagcgg ccggtgcggc
     2581 cagggcggcg agacgacccg cctgggtgag ggcggcggca aacgtgttct cccagcggct
     2641 ccactcgatg gtgaggtctt tgcaggcgcc gtcggccagg gaccaggcgg cgtcgcgggc
     2701 gaggacctcg cggctgatcc cccgcatcac cgctcccgtg ccctgcgcgc tggcggcggg
     2761 tcggtggacc agccggcgcg caggtcctcg atcgccgacg agcagagagt gatcggtgtg
     2821 acggtgagat cagggatccc agcggtcagg gccgtcacgg tgtcggttgt gcacacgccc
     2881 accacgaccc catgccgggt gatgacccag gtccgtttcg gcagggctaa gggtttggcg
     2941 ctggtggcag tgatccattc ctcgaccagg tgtcggagct cttcgtagta gtgctggtag
     3001 tggccgtggt cgatgcgctc gagtggggtg tcgtcgatcg ctgtgagcat cgccctggtg
     3061 cgctggtgca ggttctccag ggcggggttg tctatcggtc tcatccgtat ctcctcgtga
     3121 cgggggccca ggtcgcgtgg gtggtgggga tggtggtgac gtcggcgtcg gcgagcagct
     3181 gctgcccgg
//
LOCUS       DAOPJG010000278         3174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_34257_length_3174_cov_7.267393, whole genome shotgun sequence.
ACCESSION   DAOPJG010000278 DAOPJG010000000
VERSION     DAOPJG010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34257_length_3174_cov_7.267393"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="PKU75_16305"
     CDS             <1..1431
                     /locus_tag="PKU75_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82490.1"
                     /translation="DFLDDGSREDDDLIRGALDSLRHDVNDLPLPPLSQVKSRGTRRK
                     QTIWRSAGAAVAAAAVVVAAVGLSGLGRPNGDNTDPATQSTSSITRSTTSSISSTPTS
                     SATESTAPQVDWEQLLAAKPLIQPSAAEWQAAFGLAEEPTVAEPTALDTTPVCDLPVG
                     DGIALDRQEVTAAGAPSAFAHEWTWSYGSDQGAQDAAAAMGAAFDNCSRPSDMTQDFS
                     DEFEEFGKLWRFTGDNGTAWVALANAGRDVTYLEVWDSPDGSPVTKSEMARIAWVAGQ
                     RLQRYAASDAGGAGSGSSGGDLPIAGPPARLPTGTLFVDPAAYSSALLTDGAETEAGP
                     GDFEGSAQVLTACDADTAAEGEFGIVKIKESGADGSYFATQRIRKVESAQTEIDRLTT
                     GFTDCTSRVGQTTTTAYPGPDAGMVKLATVFDDGSTPLVEYAIVTATKTPGYVSTIVV
                     TVSAKEISDEPYYAELRRLGTLAAAR"
     gene            1492..2136
                     /locus_tag="PKU75_16310"
     CDS             1492..2136
                     /locus_tag="PKU75_16310"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPF82491.1"
                     /translation="MEIALALAAAVAGLAGGWLALTRIRTGFHRYDTDGPPRGLTWVV
                     PVSALAAGATAYGLRDWAWPVGVTGAVAAIVGVALAAIDLDVHRLPRVVTWPCYPAVA
                     LLLTVCSAVTGDWPALLRALEAGAALWVLYYVLHRLARRRGLGRGDVTLAGVVGMLLG
                     WFGWAQVAVATYLAFILAGASAALLLVLRRVSRSDRIAFGPAMIAAALVLLALQ"
     gene            2173..>3174
                     /locus_tag="PKU75_16315"
     CDS             2173..>3174
                     /locus_tag="PKU75_16315"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HPF82492.1"
                     /translation="MTTPARPRSRRLLGLDLARGLAVCTMILAHTSPWPIDLPAEYLT
                     APLFATLVGASLHLAWSAAQARGTSGLAFVADNGFRGACLVAIGEVLQSAYQFIDVVL
                     QTLGLLTIVLAPVVVLLGRAARPGRAALAGASALWLISPFVMEASRHWLAEHPTLDPL
                     GRAIGLWLAAGVHYRVVSFAVYGLVGVALAVWFARGGARGRDGLRAVGVCALVTVGAY
                     GGGRLIDQGQPYAGDHAELLGSTAFAAGATIGCVWLLARFGDAHRTDRPGVLAPLVAT
                     GQLALTAYSLQIVLLRVLQELTGPGFDDDRPWVLGLTLAVVVGCSWAWRRTGLTGPME
                     "
BASE COUNT          392 a         1173 c         1145 g          464 t
ORIGIN      
        1 gacttcctcg acgacgggag ccgggaggat gacgacctga tccggggcgc cctcgactcg
       61 ctgcgccacg acgtcaacga cctgcccctg ccgccgctgt cccaggtgaa gtcccgcggc
      121 acccggcgca agcagaccat atggcggtcc gccggcgccg cggtcgccgc cgcggccgtc
      181 gtggtcgcgg ccgtcggact gtccgggctc ggccggccga atggcgacaa caccgatccg
      241 gcgacgcagt ccacctcgag catcacccgt tctacgacct cgtccatctc gtccacgccg
      301 accagctcgg ccaccgaatc gactgcgcca caagtggatt gggagcagct gctcgccgcc
      361 aagcccctga tccagccgtc tgccgcggag tggcaggccg ccttcggtct cgccgaggaa
      421 cccactgtcg cggagccgac ggccctcgac accacgccgg tgtgcgactt gccggtcggc
      481 gacggcatcg cgctggaccg ccaggaggtc accgccgccg gcgccccgtc ggccttcgcc
      541 cacgagtgga cctggagcta cggcagcgat cagggcgccc aggacgccgc cgcggcaatg
      601 ggcgcggcgt tcgacaactg cagccgaccc agcgacatga cgcaggactt ctccgacgag
      661 ttcgaggagt tcggcaagct gtggcgcttc acgggcgaca acgggacggc ctgggtcgcc
      721 ctggccaacg ccgggcgcga tgtgacctac ctcgaggtgt gggactcccc cgacggcagc
      781 cccgtcacca agtcggagat ggcccggatc gcctgggtcg ccggccagcg cctgcagcgc
      841 tacgccgcct cggacgccgg cggcgccggg agcgggtcgt ccggcggcga cctgccgatc
      901 gccggcccac ccgcccgact gccgaccggc accctgttcg tcgaccccgc ggcatactcc
      961 tcggcgttgc tcaccgacgg cgccgagacc gaggcgggcc cgggcgactt cgaagggtcc
     1021 gcccaggtcc tcaccgcctg cgacgccgac accgcggcgg agggggagtt cggcatcgtc
     1081 aagatcaagg agagcggggc cgacgggagc tacttcgcca cccaacggat ccgcaaggtc
     1141 gagtcggcac agaccgagat cgaccgcctg acaacgggtt tcacggactg cacgagccgt
     1201 gtgggacaga ccacgacaac ggcatacccg ggcccggacg cggggatggt caagctcgcg
     1261 accgtcttcg acgacggcag caccccgctg gtggagtacg cgatcgtgac ggcgaccaag
     1321 accccgggat atgtgtccac catcgtcgtg accgtgtcgg cgaaggaaat ctccgacgag
     1381 ccctactacg ccgaactgcg gcgcctcggc acgctcgccg ccgcccgctg accctgtgga
     1441 tgaccgcatc cacgacacgc gcgggcgagg tcatcgccgt agggtcggcg catggagatc
     1501 gccctcgccc tggccgccgc cgtggccggc ctggccggcg ggtggctcgc cctcacgcgc
     1561 atccgcaccg gcttccaccg ctatgacacc gacggtccgc cgcgcgggct gacgtgggtg
     1621 gtcccggtga gcgcgctggc ggcgggggcg acggcatacg gcctgcgcga ctgggcctgg
     1681 ccggtcgggg tgaccggcgc ggtggccgcg atcgtcggcg tcgcgctcgc ggcgatcgac
     1741 ctcgacgtgc accggcttcc gcgggtggtg acctggccgt gctatcccgc cgtcgcgctg
     1801 ttgctcacgg tatgcagcgc cgtcaccggc gactggccgg cgctgctgcg tgcgctggag
     1861 gcgggggcgg cgctgtgggt gctctattac gtcctgcacc ggctggcgcg gcggcgcggg
     1921 ctgggccggg gagacgtcac gctcgcggga gtggtcggga tgttgctcgg ctggttcggc
     1981 tgggcccagg tcgcggtcgc gacctatctc gcgttcatcc tcgccggcgc gtccgcggct
     2041 ctgctgctgg tgttgcggcg ggtctcgcgc agcgaccgga tcgccttcgg gccggccatg
     2101 atcgccgccg cgctggtgct gctcgccctg cagtgacgac acccgcggca caatgaccgc
     2161 accatgtccg ccgtgaccac gcccgcccgg ccccgcagcc gccggctgct cggcctcgac
     2221 ctggctcgcg ggctcgcggt gtgcacgatg atcctggccc acacgtcgcc gtggccgatc
     2281 gacctgcccg ccgaatacct caccgccccg ctcttcgcca ccctcgtcgg cgcatccttg
     2341 cacctggcgt ggtcggccgc gcaggcccgg ggcacctccg ggctcgcctt cgtggcggac
     2401 aacggtttcc gcggcgcgtg cctcgtcgcg atcggagagg tgctgcagtc cgcctaccag
     2461 ttcatcgacg tcgtcctgca gaccctcggc ctgctgacga tcgtgctcgc cccggtggtg
     2521 gtcctgctcg ggcgggccgc gcggcccggg cgggcggcgc tggccggggc gagcgccctc
     2581 tggctgatct cgccgttcgt catggaggcg agccggcatt ggctggccga gcacccgacc
     2641 ctcgacccgc tggggcgcgc gattgggctg tggctcgccg cgggggtgca ctaccgggtg
     2701 gtgtccttcg cggtctacgg cctggtagga gtcgcgctgg cggtctggtt cgcgcgtggc
     2761 ggcgctcgag ggcgggacgg tctgcgggcc gtcggggtgt gcgccctcgt gacggtcggg
     2821 gcgtacggcg gcggccggct catcgaccag ggccagccgt atgccggcga tcacgccgaa
     2881 ctgctcggtt ccacggcgtt cgccgctgga gcgacgatcg ggtgcgtctg gctgctcgcc
     2941 cgcttcggcg acgctcaccg caccgaccgg ccgggcgtgc tcgcgcccct ggtggcgacg
     3001 ggacaactcg cgctcacggc atacagcctg cagatcgttc tcctgcgggt tctccaggaa
     3061 ctgaccggtc caggcttcga cgacgaccgg ccgtgggtgc tcggcctgac gctcgccgtc
     3121 gtcgtcgggt gctcctgggc gtggcggcgc accgggctca ccgggccgat ggag
//
LOCUS       DAOPJG010000279         3094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_35556_length_3094_cov_7.833827, whole genome shotgun sequence.
ACCESSION   DAOPJG010000279 DAOPJG010000000
VERSION     DAOPJG010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35556_length_3094_cov_7.833827"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKU75_16320"
     CDS             <1..528
                     /locus_tag="PKU75_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPF82493.1"
                     /translation="TRGGVKRVRFGGDDTVIGDMGSWRYEDESEAADGMDALVHVDGH
                     QPFSALRLDLGNPHTVVAVPPSVDLDELDLTTAPQVNPNPVAGTNVEFVKPLGRGHIR
                     MRVHERGVGETESCGTGICAAAIATHLWAGDLDGDEPWIVDVPGGRLQVRLLPDNHVE
                     LSGPAVLVADGTVQI"
     gene            complement(525..1133)
                     /locus_tag="PKU75_16325"
     CDS             complement(525..1133)
                     /locus_tag="PKU75_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPF82494.1"
                     /translation="MTGDVEHYFTAQPASPAQLRERSVTLAGKSINVVVASGVFSPDG
                     VDRGTAVLLNEVPDPPGVGTFLDLGCGWGPIALTLALRSPGAIVYGVDVNERALDLLR
                     RNASRHGLSWIRAVAPDAVPADVRFDLIWSNPPIRIGKAALHELLQSWLPRLAPGGTA
                     YLVVAKQLGADSLQKWIAKQWPDFAVDRYASDKGFRVLRVQG"
     gene            complement(1170..2693)
                     /gene="glpK"
                     /locus_tag="PKU75_16330"
     CDS             complement(1170..2693)
                     /gene="glpK"
                     /locus_tag="PKU75_16330"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPF82495.1"
                     /translation="MTEATYILAIDQGTTSTRAILFDRAGSVVAVDQREHEQIFPRPG
                     WVEHDGLEIWENTRMVVGGVLGKANVNRGAVAAVGITNQRETTLVWEKETGRPIANAV
                     VWQDTRTGRLVEELAGDAGPRRYAAQTGLPLATYFAGPKIRWLLDHVDGARERAEAGE
                     LLFGTMDTWILWNLTGGPDGGLHLTDVTNASRTLLMDLRSLQWDPALCAALGVPMGML
                     PQIRSSSEVYGQCHPGILDGVPIAGILGDQQAATFGQGCLERGSAKNTYGTGNFMLLN
                     TGTDIVASDNGLLTTVCYQLGDQPAVYALEGSIAVTGSLIQWLRDNLGLIPSAPEVEG
                     LALSVADNGGTYFVPAFSGLFAPYWRPDARGAIVGLTRFVTKGHIARAALESTAYQTH
                     DVLEAMNADSGVPLRELKVDGGMTANDTLMQFQADILGVDVIRPKVAETTALGAAYAA
                     GLAVGYWGSTGDILANWQEDRRWSAGMEPAERDRLLRGWAKAVHRTLDWVDDDVEQG"
     gene            2768..>3094
                     /locus_tag="PKU75_16335"
     CDS             2768..>3094
                     /locus_tag="PKU75_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPF82496.1"
                     /translation="MATPPRMDPSYRAKAWTRLGQREFDVLVIGGGVTGSGAALDAAT
                     RGLSVALVEQRDFASGTSSRSSKLMHGGVRYLEQMDFQLVREALKERELALTRLAPHL
                     VKPLPFL"
BASE COUNT          474 a         1086 c         1048 g          486 t
ORIGIN      
        1 acccgcggcg gcgtcaagcg ggtgcgcttc ggtggcgatg acaccgtcat cggcgacatg
       61 ggcagctggc gttatgagga cgagtccgag gcggccgacg gcatggacgc cctcgtccac
      121 gtcgacggcc accagccgtt ctccgcgctg cgtctcgatc tcgggaatcc gcatacggtc
      181 gtcgccgtcc cgccgtccgt cgacctcgac gaactcgacc tcacgacggc gccgcaggtc
      241 aacccgaatc cggtggccgg caccaatgtc gagttcgtga aaccgttggg gcgcgggcat
      301 attcggatgc gcgtgcacga gcgtggagtc ggcgagaccg agtcgtgcgg caccggcatc
      361 tgcgccgcgg ccatcgcgac ccacctgtgg gccggagacc tcgacggcga cgagccgtgg
      421 atcgtcgacg tcccgggcgg ccggctccag gtccggctgt tgccggacaa ccacgtcgag
      481 ctctcggggc ctgccgtcct cgtcgccgac ggcaccgtgc agatctagcc ctgcacgcgc
      541 aggacccgga agcccttgtc gctggcgtac cggtccacgg cgaagtccgg ccactgcttc
      601 gcgatccatt tctgcaacga gtccgcgccg agctgtttcg cgacgacgag gtatgcggtg
      661 ccgcccggcg caagcctcgg caaccagctc tggagcagct cgtgcagcgc ggccttgccg
      721 atccggatcg gcgggttgga ccagatcagg tcgaagcgca catctgcggg gaccgcgtcg
      781 ggtgcaaccg cgcgaatcca cgacagcccg tggcgagagg cgttgcggcg cagcagatcc
      841 agcgcgcgct cgttgacgtc gacgccatag acgatcgcgc caggagagcg cagcgcgaga
      901 gtcagggcga tcgggcccca gccgcagccg aggtcgagga aggtgccgac accgggcggg
      961 tccggcacct cgttcagcag gacagccgtg cctcgatcca ccccgtcggg ggagaaaacc
     1021 cctgatgcca caacgacatt gatcgatttg cccgcgagcg tcacggatcg ctcacgcagc
     1081 tgggcggggc tcgccggttg tgcggtgaaa tagtgctcga cgtcgcctgt catgtggatg
     1141 tgcctctgcc tcgagtcctg tgcgcccgat cagccttgct cgacgtcgtc gtcgacccag
     1201 tcgagcgtgc ggtgcacggc cttggcccac ccgcgcagca aacggtcacg ctcggccggc
     1261 tccatgccgg ccgaccagcg ccggtcctcc tgccagttgg cgaggatgtc gccggtcgat
     1321 ccccaatagc ccaccgcgag ccccgcggca taggccgcac ccagggccgt cgtctcggcc
     1381 accttgggcc ggatgacgtc gacgccgagg atgtcggcct ggaactgcat cagggtgtca
     1441 ttggccgtca tcccgccgtc gaccttcaat tctcgcagcg gcacaccgga atcggcgttc
     1501 atcgcctcca gcacgtcatg ggtctggtat gccgtggact ccagggcggc gcgcgcgatg
     1561 tgccccttgg tgacgaaccg ggtcagcccg acgatcgcgc cgcgggcgtc cgggcgccag
     1621 tagggcgcga acaggccgga gaaggccggc acgaagtagg ttccgccgtt gtccgcgacc
     1681 gacagggcaa ggccttcgac ctcgggtgcg gaggggatga ggccgaggtt gtcgcgcagc
     1741 cactggatga gggagccggt gaccgcgatg gacccctcca gcgcatacac ggccggctgg
     1801 tcgcccagct gataacagac ggtcgtcagc agcccgttgt cggaggccac gatgtcggtg
     1861 ccggtgttga gcaacatgaa attgccggtg ccgtaagtgt tcttggcgct gccgcgctcc
     1921 aggcagccct ggccgaaggt ggcggcctgc tggtcgccga ggatgcccgc gatcgggacg
     1981 ccgtcgagga tgccggggtg gcactgccca tacacctcgc tggaggagcg gatctgaggc
     2041 aacatcccca tcgggacgcc gagggccgcg cagagcgccg gatcccattg cagggagcgc
     2101 agatccatca agagggtacg gctggcgttc gtcacgtccg tcaggtgcag accgccgtcg
     2161 ggcccgccgg tgagattcca caggatccag gtgtccatcg tcccgaagag cagttccccg
     2221 gcctcggcgc gctcccgcgc gccgtcgacg tgatcgagca gccagcggat cttgggcccg
     2281 gcgaaatagg tggcgagcgg gagaccggtc tgcgccgcat accgccgtgg gcctgcgtca
     2341 ccggccagct cctccaccag ccgccctgtc cgggtgtcct gccagacaac cgcattcgcg
     2401 atcggccgcc ccgtctcctt ctcccacacc aaggtggtct cgcgttggtt ggtgatgccg
     2461 acggcggcca cggccccccg gttgacgttc gccttgccga gcaccccgcc gaccaccatc
     2521 cgggtgttct cccaaatctc cagtccgtcg tgctcgaccc agcccgggcg ggggaagatc
     2581 tgctcgtgct cgcgctggtc gaccgcgacc acgctgccgg cgcgatcgaa gaggatggca
     2641 cgggtgctcg tcgtcccctg gtcgatcgcc aggatgtatg tggcctcggt cacgagtagg
     2701 ccctccgtcg cctcgatcac gtcgtcttga tccgtcggca ccctaaccgt cttgacacaa
     2761 tgcggccatg gcgacaccac cgcggatgga tccgtcctat cgcgccaagg cctggactcg
     2821 gctcgggcag cgcgagttcg acgtcctcgt gatcggcggc ggggtgaccg gcagcggtgc
     2881 cgccctggac gcggccaccc gagggctctc ggtcgccctg gtcgaacagc gtgacttcgc
     2941 ctccggcacc tcctcccgct ccagcaagct catgcacggc ggcgtgcgct atctggagca
     3001 gatggacttc caactcgtcc gggaggccct caaggagcgc gaactcgccc tgacccgcct
     3061 cgcgccgcac ctggtcaaac cgctgccgtt cctg
//
LOCUS       DAOPJG010000280         3089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_35632_length_3089_cov_6.076796, whole genome shotgun sequence.
ACCESSION   DAOPJG010000280 DAOPJG010000000
VERSION     DAOPJG010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35632_length_3089_cov_6.076796"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /gene="ettA"
                     /locus_tag="PKU75_16340"
     CDS             <1..1318
                     /gene="ettA"
                     /locus_tag="PKU75_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPF82497.1"
                     /translation="EMGQLQEKIDSADAWDLDSQLEQAMDALRCPPPDADVSVLSGGE
                     KRRVALCKLLLEKPDLLLLDEPTNHLDAESVLWLEQHLAAYQGCVVAVTHDRYFMDNV
                     AEWILELDRGRAYPYEGNYSTYLEKKRARLEVQGKKDAKLAKRLQEELEWVRSNAKAK
                     QTKSKARLQRYEEMAAEAERTRKLDFEEIAIPPGPRLGSQVIEVRDLEKGFGDRILID
                     DLSFTLPRNGIVGVIGPNGVGKTTLFKTIVGLEEADEGSVKIGETVKISYVDQSRAGI
                     DPEKNLWEVVSDGLDFIKVGNVEIPSRAYVSQFGFKGPDQQKKAGVLSGGERNRLNLA
                     LTLKQGGNLLLLDEPTNDLDVETLGSLENALLDFPGCAVVISHDRWFLDRVATHILAW
                     EGTDEDPSKWFWFEGNFEAYEKNKVERLGEEAARPHRVTYRKLHRD"
     assembly_gap    1325..1424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1477..3060)
                     /locus_tag="PKU75_16345"
     CDS             complement(1477..3060)
                     /locus_tag="PKU75_16345"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPF82498.1"
                     /translation="MDNFLAEQPILLLFLLLAIGTLLGKISIRGVGLGPAGVLFAALA
                     FSAIHPDWAVPEVVGNLGLALFAYTIGIVAGPSFFSGLRRGARPIGLVAVTLVALGAL
                     TYGIGRGLGFSSGTIAGLYAGSNTNTPGLAAAIIRLDGSPEPTVGYSLSYVGGVLVML
                     LTAAYVVRAGRAAPDPEAAANRPEVETRVIEITHDNVMDISELHRTPYGEVLFSRHRT
                     PDGEMHMASGETVLRPGDLVHLVGSERALNHLTKQLGRVSAAVLTMERDEFDFRRIIL
                     SDKALFGKTIGELHLWDRFMARATRVRRGDQDFLATEHLIVQPGDRIRVTAPADQMGK
                     VAKFLGDSEHAISDINPLGLATGMALGLLLGTVPFVVPGLGTLHLGAAAGPLLVGLVV
                     GRLGRTGRLLWTLPHSGAETLTQLGLLLFLAYAGGRAGSQVVTAITSPLGVKLVLAGL
                     VITAAHAAVLALGARQLALAAPRTAGILAGSQTQPAVLAYANESTGHDPRVTLGYALV
                     YPVAMVVKIMACQILTMLP"
BASE COUNT          543 a          986 c         1048 g          412 t
ORIGIN      
        1 cgagatgggg cagttgcagg agaagatcga ctccgccgac gcctgggatc tcgactcgca
       61 actcgagcag gccatggacg ccctgcgctg cccgccgccg gacgccgacg tctccgtcct
      121 ctccggtggc gagaagcgcc gcgtcgcgtt gtgcaagctg ctgctggaga agcccgacct
      181 gctgctgctc gacgagccca ccaaccacct cgacgccgag tcggtcctgt ggctcgaaca
      241 gcacctcgcg gcataccagg ggtgcgtcgt cgccgtcacc cacgaccgct acttcatgga
      301 caacgtggcc gagtggatcc tcgaactcga ccgcggccgc gcctatccct atgagggcaa
      361 ctactcgaca tacctggaga agaagcgcgc ccgcctggag gtccagggca agaaggacgc
      421 caagctcgcc aagcggctcc aggaggaact ggagtgggtg cgctccaatg ccaaggcgaa
      481 gcagaccaag tccaaggcgc gcttgcagcg gtatgaggag atggcggccg aggcggagcg
      541 gacccgcaag ctcgacttcg aggagatcgc gatcccgccg ggcccgcggc tcggctcaca
      601 agtcatcgag gtccgcgacc tcgagaaggg gttcggcgac cgcatcctga tcgacgacct
      661 gtccttcacc ttgccccgca acggaatcgt cggcgtcatc ggccccaatg gcgtcggcaa
      721 gacgaccctc ttcaagacca tcgtggggct cgaggaggca gacgagggca gcgtcaagat
      781 cggcgagacc gtcaagatct cgtatgtcga ccagtcccgc gccggcatcg acccggagaa
      841 gaatctgtgg gaggtcgtct ccgacgggct cgacttcatc aaggtcggaa acgtcgaaat
      901 cccctcgcgc gcatacgttt cgcagttcgg gttcaaaggt ccggaccagc agaagaaggc
      961 cggcgttctc tccggtggcg agcgcaaccg gctcaacctc gcgttgacct tgaagcaggg
     1021 cggcaacctg ctcctgctcg acgagccgac caacgacctc gacgtcgaaa ccctcggctc
     1081 gctcgagaac gccctgctcg acttccccgg ctgcgccgtg gtcatcagcc acgaccggtg
     1141 gttcctcgac cgggtcgcca cccacatcct cgcgtgggag ggaaccgacg aggatccgtc
     1201 caagtggttc tggttcgagg gcaacttcga ggcctacgag aagaacaagg tcgagcgcct
     1261 cggcgaggaa gcggcccgcc cgcaccgcgt gacctatcgc aagctgcacc gcgactgagc
     1321 ctggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctcccg ccagcggccg
     1441 tcgcgccagc ggccgccgcc catacgacgc ggccggctac ggcaacatcg tcaggatctg
     1501 acaggccatg atcttgacca ccatggcgac ggggtagacc agggcgtacc ccagggtgac
     1561 gcgggggtcg tggcccgtgg actcgttggc gtaggccagc acagccggct gggtctgcga
     1621 acccgccagg atgccggccg tccggggggc ggcgagggcg agctggcggg caccgagggc
     1681 gaggacggcg gcgtgagccg ccgtgatgac caggccggcg agcacgagtt tgacaccgag
     1741 cggcgaggtg atggcggtga ccacctggct accggcgcgg ccaccggcat acgccaggaa
     1801 cagcagaagc cccagctggg tgagggtctc ggcacccgaa tgcggcagcg tccacagcag
     1861 ccggccggtt cgacccagac gcccgacgac gagccccacc agcaagggtc cggccgccgc
     1921 acccaggtgc aaggtcccga ggccgggcac gacgaagggc acggtcccga gcaggaggcc
     1981 gagcgccata cccgtggcca ggcccagcgg gttgatatcg gagatggcgt gctcggaatc
     2041 cccgaggaac ttcgcgacct tgcccatctg gtccgcgggg gcggtgaccc ggatgcggtc
     2101 gcccggctgg acgatgaggt gctcggtggc aaggaagtcc tggtcgccgc ggcgcacgcg
     2161 cgtcgcccgc gccatgaacc ggtcccacag gtgcagctca ccgatcgtct tgccgaacaa
     2221 tgccttgtcg gacaggatga ttcgccggaa gtcgaattcg tcgcgttcca tcgtcaacac
     2281 ggcggcgctc acccggccga gttgtttggt caggtggttc agcgcccgct cggaccccac
     2341 gagatgcacg agatcgccgg ggcggagcac ggtctcgccg gacgccatgt gcatctcgcc
     2401 gtcgggggtg cggtggcggg agaagagcac ttcaccgtat ggggtgcggt gcagttcgct
     2461 gatgtccatc acgttgtcgt gggtgatctc gatgacgcgg gtctcgacct ccggccggtt
     2521 cgccgcggct tcgggatcgg gtgcggcccg gccggcgcgc accacgtatg ccgcggtgag
     2581 cagcatcacc agcaccccac cgacatacga cagtgagtag ccgaccgtcg gctccggtga
     2641 gccgtcgagg cggatgatcg cggcggcgag gccgggggtg ttcgtgttgg acccggcata
     2701 caacccggcg atggtgccgg agctgaagcc gagcccgcga ccgatgccgt aggtcagcgc
     2761 gccgagcgcg acgagggtga cggcgacgag cccgatgggc cgggcgccgc gtcggaggcc
     2821 ggagaagaag gagggtccgg ccacgatccc gatggtgtag gcgaaaagcg cgaggccgag
     2881 gttgccgacc acctctggaa cggcccagtc ggggtggatg gcggagaagg cgagggcggc
     2941 gaagagcacg cccgccggcc cgagaccgac gccccggatg ctgatcttgc cgagtagtgt
     3001 cccgatcgcc agcaacagaa acaggagcag gatcggttgc tcggcgagga agttatccac
     3061 gtgtcaccag aatacaggcc gcgcagggc
//
LOCUS       DAOPJG010000281         3086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_35691_length_3086_cov_10.850214, whole genome shotgun
            sequence.
ACCESSION   DAOPJG010000281 DAOPJG010000000
VERSION     DAOPJG010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35691_length_3086_cov_10.850214"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKU75_16350"
     CDS             complement(<1..376)
                     /locus_tag="PKU75_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPF82499.1"
                     /translation="MAKELEFNDSARKALERGVDALANAVKVTLGPKGRNVVIDKKWG
                     APTITNDGVTIAKEIELEDAYENLGAQLAKEVATKTNDIAGDGTTTATVLAQAMVKEG
                     LRNVAAGAAPSGLKRGMDKAVQA"
     gene            complement(530..826)
                     /gene="groES"
                     /locus_tag="PKU75_16355"
     CDS             complement(530..826)
                     /gene="groES"
                     /locus_tag="PKU75_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPF82500.1"
                     /translation="MSVSIKPLEDRIVIKSLEAEQTTASGLVIPDTAKEKPQEGEVLA
                     VGPGRWNEDGDERIPLDVKVGDRVIYSKYGGTEIKHGGEEYLILSARDVLAIVG"
     gene            complement(1009..1767)
                     /locus_tag="PKU75_16360"
     CDS             complement(1009..1767)
                     /locus_tag="PKU75_16360"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HPF82501.1"
                     /translation="MRWIVTGMNGTVAPRLAAALRARGEKVLAWDRARVPPGDEGAMR
                     AFVDESAAGGICHLAMGAPEWAASMARLCAERGMPYLFTSSVSVFAATQRGPLGVEVV
                     PEASDDYGRYKQECERQVAAAHPEAIIARLGWQIGDAPGSNTMTDYLTRAAADGAGRV
                     EASTRWIPSSCFLDDTAAALVHLLDRGEGGLFQVEGNPDGLSLNEIATRLAGRLEAGW
                     DVIPTDDVVMDNRMLDPRVPVRSLSERLSRLTGS"
     gene            complement(1780..2085)
                     /locus_tag="PKU75_16365"
     CDS             complement(1780..2085)
                     /locus_tag="PKU75_16365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPF82502.1"
                     /translation="MTTRPLSEDEWELYREIRLRALADTPSAFGSTLERERGFTEADW
                     RQRMRGPMVVVEEEGDPVASGGTFEDADGVTCVWGMWTEPTSRGRGHATAILHGLPG"
     gene            2157..>3086
                     /locus_tag="PKU75_16370"
     CDS             2157..>3086
                     /locus_tag="PKU75_16370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPF82503.1"
                     /translation="MDVDLVRWLGSPGGRDLLRSLGSYAEARVPAESTRLRGLGLSAE
                     QVAALLTQQRLRTRAHEKFGDLADDLLFTADGLEQASRPEVAAGHAERFAAAGLERVH
                     DLGCGVGADAIALAIAGVRVEAIDADPVTAAIAGANLRPWPGSTAEVGLAEDFRPPAD
                     RDRIGAWLDPARRTTGAADIRGRTRRVFRLDDICPSWDVVRGIAAEVPATGVKLSPSF
                     PHNEIPPGAESQWVSRAGTVLECAIWWGPLVRSPGRTARILRPGGPPVDVTEEDATDD
                     ASALTGAGGLGPWLYEVDRALVQAGLISAVTAAT"
BASE COUNT          395 a         1101 c         1095 g          495 t
ORIGIN      
        1 cggcctgcac ggccttgtcc atgccccgct tgaggccgga cggagcggca ccggccgcga
       61 cattgcgcag gccctccttg accatcgcct gggcgaggac cgtcgcggtc gtcgtcccgt
      121 cgccggcgat gtcgttggtc ttggtcgcga cctccttggc gagctgggcg ccgaggttct
      181 cgtaggcgtc ctcgagctcg atctccttgg cgatggtcac gccgtcgttg gtgatcgtcg
      241 gggcgcccca cttcttgtcg atgacgacat tgcggccctt ggggccgagc gtcaccttga
      301 cggcgttggc gagggcgtcc acgccccgct cgagcgcctt gcgggcggag tcgttgaact
      361 ccagttcctt ggccattgag cagtcctttt tcgagtggcg ttgcgtccgc gtcgacacgt
      421 gctgggacgt gcgttcacgc ggacgcgacg gaagtgcgga agggcgtatg ccgtgggctc
      481 accgcggggg cgagcccacg gcatacgtgg tggtgctgtg ggtggtgggt cagccgacga
      541 tggcgaggac gtcgcgcgcg gagaggatga ggtactcctc gccgccgtgc ttgatctcgg
      601 tgccgccgta cttgctgtag atgacccggt cgccgacctt gacgtcgagc gggatgcgct
      661 cgtcgccgtc ctcgttccag cggccggggc cgacggcgag aacctcgccc tcctgcggct
      721 tctccttggc cgtgtccggg atgaccaggc cggacgccgt cgtctgctca gcctcgaggg
      781 acttgatgac gatgcggtct tcgagcggct tgatggaaac cgacacgatg actccttcga
      841 tgtctattgc ggatgaatgg tgtgcagttc tggtgtgcac tccggccgcc gtcgcggggg
      901 tcgaccgttg cgtcacattg ggtgtcacat tggcactcgc ctagggagag tgccagtcag
      961 aatctatgcc gcggattagc actcggtcaa ctcgagtgcc agcactcctc aggagcccgt
     1021 gaggcggctc aggcgctcgc tgagggagcg gaccgggacg cgagggtcga gcatccggtt
     1081 gtccataacg acgtcgtcgg tggggatgac gtcccatccc gcctcgaggc ggccggccag
     1141 ccgggtcgcg atctcgttga gggacaagcc atccggattg ccctccacct ggaacagccc
     1201 gccctccccg cggtcgagca ggtgcacgag cgcggcagcg gtgtcgtcga ggaagcagct
     1261 cgacgggatc caccgggtgc tggcttcgac gcggccggca ccgtccgcgg ccgcgcgcgt
     1321 cagatagtcg gtcatcgtgt tggaccccgg cgcgtcgccg atctgccacc cgagtcgcgc
     1381 gatgatcgcc tccggatggg cggcagcgac ctgccgttcg cactcctgct tgtaacgccc
     1441 gtagtcgtcg ctcgcctccg gcacgacctc gactcccagc ggcccgcgct gggtcgcggc
     1501 gaagacggac accgaactgg tgaacagata cggcataccg cgctcggcgc acagccgcgc
     1561 catcgaggcc gcccattcgg gcgcccccat cgcgaggtga cagatcccgc ccgccgcgga
     1621 ctcgtccacg aaggcccgca tggcgccctc gtcgccgggg ggcacccgcg cgcggtccca
     1681 cgcgaggacc ttctcgccac gcgcgcgcaa ggcggccgcc aggcgcgggg cgaccgtgcc
     1741 attcattccc gtcacgatcc accgcatccg gcaatcctct cagcccggca ggccatgcag
     1801 gatcgcggtg gcatggccgc gaccgcgcga cgtgggttcg gtccacatcc cccagacgca
     1861 cgtgaccccg tcggcatcct cgaaggtccc cccgctggcc accggatcgc cctcctcctc
     1921 gacgaccacc atcggcccgc gcatccgctg ccgccagtcc gcctcggtga agccccgctc
     1981 ccgctccagg gtggagccga acgccgaggg ggtgtcggcg agcgctcgca gccggatctc
     2041 gcggtagagc tcccactcgt cctcgctcag cgggcgcgtt gtcatggccg gacgctgcca
     2101 ggcggtatgc cgccgccgca acacgatttc ccgcgcgccg gtgttggatg gagcccgtgg
     2161 acgtggacct ggtgcgctgg ctcgggtcgc ccggcgggcg cgacctgctg cgcagcctcg
     2221 ggtcgtatgc cgaggcgcgc gttcccgctg agtccacccg cctgcggggg ctgggcctga
     2281 gtgccgagca ggttgcggcg ttgttgacgc agcagcggtt gcgcacgcgg gcgcacgaga
     2341 agttcggcga tctcgccgac gatctgctgt tcaccgccga cggcctggag caggccagcc
     2401 ggccggaggt ggccgccggc cacgccgagc gcttcgccgc ggccgggctc gagcgcgtgc
     2461 acgacctcgg gtgcggggtc ggcgccgacg ccatcgcgct cgcaatcgcc ggcgtgcggg
     2521 tcgaggcgat cgatgcggat ccggtgacgg ccgcgatcgc cggcgcgaac ctgcgcccgt
     2581 ggcccggcag caccgccgag gtcggcctgg ccgaggactt ccgtccgccc gcggaccggg
     2641 atcggatcgg cgcctggctc gaccccgcgc ggcgcaccac cggtgccgcg gacatcaggg
     2701 gccgaacccg gcgcgtgttt cgcctcgacg acatctgccc ctcgtgggac gtggttcgcg
     2761 gcatcgctgc cgaggtcccg gcgaccggcg tcaaactgtc accgtcgttc ccgcacaacg
     2821 agattccgcc cggagcggag tcccagtggg tgtcccgggc cggcacggta ctggagtgcg
     2881 cgatctggtg gggaccgctc gtccgctccc ccggccgtac cgctcgcatc ctccgtcccg
     2941 gcggcccgcc ggtggacgtg acggaggagg acgcgaccga tgacgcgtcg gctctcaccg
     3001 gcgccggcgg cctcggtccg tggctgtacg aggtggatcg cgccctcgtc caggcgggcc
     3061 tcatcagcgc cgtgaccgcc gccacg
//
LOCUS       DAOPJG010000282         3047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_36327_length_3047_cov_9.136698, whole genome shotgun sequence.
ACCESSION   DAOPJG010000282 DAOPJG010000000
VERSION     DAOPJG010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36327_length_3047_cov_9.136698"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1269)
                     /gene="dusB"
                     /locus_tag="PKU75_16375"
     CDS             complement(79..1269)
                     /gene="dusB"
                     /locus_tag="PKU75_16375"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPF82504.1"
                     /translation="MTASLLPAPAARPLRLGPLTIASPVVLAPMAGITNRAFRRLCRE
                     YGGAGGSGATSLYVSEMITSRALVERNELTMRLIEHDPDESPRSTQIYSVDPVTTAAA
                     VRILVQEDRADHIDLNFGCPVPKVTRKGGGGALPWKRELFRSIVAAAVREAEPYDVPV
                     TVKMRMGIDDGHLTYLDAGRIAASSGAAAVALHARTVSQAYSGTADWSAIARLKEAVP
                     GIPVLGNGDIWSAEDALRMVAETGCDGVVVGRGCLGRPWLFADLAAAFAGRPERVTPT
                     LGEVGATLRRHAAYLCDFYEDEAKGCRDIRKHIAWYLKGFPAGSTIRHRLALVNTLAD
                     LDDLIADLDPEAPWPGADAEGQRGRSGNPRHVVLPEGWLDSPDLTPEQRALVAQAEDD
                     VSGG"
     gene            1362..2249
                     /locus_tag="PKU75_16380"
     CDS             1362..2249
                     /locus_tag="PKU75_16380"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HPF82505.1"
                     /translation="MTAGAVAALVALAGCSGGSGGGRVRVTYSGSASTASTSTASAST
                     ASASSTPETTTSASSTSAGATEGGDEAPDTREAIPGPEVEVAALSKAPTIDGDQREWE
                     KVPAYSVQTQVGGKGSTKATSSWRLGWDENALYLFARAADPALTQTHVQRPWLIGTGD
                     AIGLEIGTFRDRVSTTKLDAQDTRILIAPNETGGTLRAIAKADGDDFDQGTTWTSGSA
                     VAKRTASGWVVEAAIPWSDLGQKPKAGLTLAGQLLQADAVASGAKRGSLAALLSNNPQ
                     RRGSGLKWRFAWGQLTLQK"
     gene            2295..>3047
                     /locus_tag="PKU75_16385"
     CDS             2295..>3047
                     /locus_tag="PKU75_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPF82506.1"
                     /translation="MTTLITPDGLHAALAGERPPLLLDAQYNLTGTPGRELYAAAHLA
                     GALFVDVDLDLADPPGPGGRHPLPDMTRLQGTLRRLGINDTSPVVVYDQGSGMGSARA
                     WWLLTYVGLADVRVLDGGLAAWTSAGYAVTDAPSPPPVPGGVTVRPGALPVLDAAAAA
                     EVARRGILLDARAAERFAGRTEPIDAVAGHIPGARNAPLDEFTGADGRLLDKERLHAY
                     FASKGVFDSAEVGAYCGSGITAARMALYCGRSA"
BASE COUNT          458 a         1112 c         1079 g          398 t
ORIGIN      
        1 tatgccgacg actcgggcca gatccgcaaa tggacgcaca agaccggcgc gatcgcgctg
       61 atcagcacca ggtgaggctc acccaccgct gacatcgtcc tcggcctgcg ccacgagcgc
      121 tcgttgctcg ggcgtcaggt cggggctgtc cagccagccc tcgggcaaca cgacatgccg
      181 cgggttgccc gagcggccgc gctggccctc ggcgtcggcg cccggccagg gcgcctcggg
      241 gtcgagatcg gcgatcaggt cgtcgagatc ggccagggtg ttgacgagcg ccagccggtg
      301 gcggatcgtc gaaccggccg gaaagccctt gagataccag gcgatgtgct tgcggatgtc
      361 gcggcagccc ttcgcctcgt cctcatagaa gtcgcagagg tatgccgcgt gccgccgcag
      421 cgtggcgccc acctcaccga gggtgggcgt cacgcgctcg ggccggccgg cgaaggccgc
      481 cgccagatcg gcgaaaagcc atgggcggcc gaggcatccg cgcccgacga cgacgccgtc
      541 gcagccggtc tcagcaacca tccgaagcgc atcctcggcc gaccagatgt cgccatttcc
      601 gaggacgggg atgccgggca cggcctcctt gagccgggcg atcgcgctcc agtccgccgt
      661 gccgctgtag gcctgcgaga ccgtgcgcgc gtgcagagcc accgccgccg cccccgacga
      721 ggcggcgatc cgcccggcgt cgagataggt caggtgtccg tcgtcgatgc ccatgcgcat
      781 cttcacggtg accggcacgt catacggctc ggcttcccgc accgccgcgg cgacgatcga
      841 gcggaacagc tcgcgcttcc acggcagcgc tcccccgccg cccttgcgcg tcaccttcgg
      901 cacggggcat ccgaagttga gatcgatgtg atcggcccgg tcttcctgca cgaggatccg
      961 cacggcggcg gccgtcgtca ccgggtcgac gctgtagatc tgggtggagc ggggcgattc
     1021 gtccggatcg tgctcgatga gccgcatcgt caactcgttg cgctccacga gcgcccgcga
     1081 ggtgatcatc tcgctgacat acagcgaggt cgcccccgac ccgccggcgc cgccgtactc
     1141 ccggcacagg cgccggaagg cgcggttggt gatcccggcc atcggcgcga gtacgacggg
     1201 cgaggcgatg gtgagcggcc cgaggcgcag cggccgggct gcgggtgcgg gcaggaggga
     1261 tgcggtcatg accgggaata ttgtcccatt ctcgcgggtg ggctccgggc aagttatcgt
     1321 gaccgcactg gggacaggga gaaatagcga tgagcagacg tatgacggcc ggtgccgtcg
     1381 ccgcgctggt tgcgctcgcc gggtgttccg gtgggtcggg cgggggccgc gtccgagtga
     1441 cctactcggg cagcgcctcg acggcgagca cgtcaaccgc gagcgcgtcg acggcgagcg
     1501 cctcgagcac gccggagacg acgacgtccg cctcgtccac ctcggccggc gccaccgagg
     1561 gcggggacga ggcccctgac acccgcgagg ccatccccgg gcccgaggtc gaggtggcgg
     1621 ccctgtcgaa ggccccgacg atcgacggcg accagcgcga gtgggagaag gtcccggcat
     1681 acagcgtgca gacccaggtc gggggcaagg gcagcaccaa ggcgacctcc tcgtggcgcc
     1741 tcggctggga cgagaacgcc ctctacctct tcgcccgcgc ggccgacccg gcgctgaccc
     1801 agacgcacgt gcaacggccg tggctcatcg gcaccggcga cgcgatcggc ctggagatcg
     1861 ggaccttccg cgaccgcgtc agcaccacca aactcgatgc ccaggacacg cgaatcctga
     1921 tcgcccccaa cgaaaccggc ggcaccttgc gcgcgatcgc caaggccgac ggcgacgact
     1981 tcgaccaggg cacgacctgg acgagcggat cggccgtcgc caaacggacg gcgagcggat
     2041 gggtcgtcga ggcagccata ccgtggtcgg atctgggcca gaaacccaag gccggactca
     2101 ccctcgcggg ccagcttctc caggccgacg cggtcgcgag cggcgccaag cgcggctctc
     2161 tcgccgccct gctgtccaac aacccgcagc ggcgcggcag cggactgaag tggcggttcg
     2221 cgtgggggca gttgactctg cagaagtgac ccgacgaagg ccgcccgccg ggtgaccggt
     2281 gccaggatgg gtgcatgacg acgctcatca ctcccgacgg cctgcacgcc gccctcgcgg
     2341 gtgagcgccc accgctgctg ctcgacgcgc agtacaacct caccggcacg ccgggacggg
     2401 agctgtacgc cgccgcccac ctcgccggtg cgctgttcgt cgatgtcgac ctcgacctcg
     2461 ccgacccgcc cggtcccggc ggtcgccatc ccctgccgga catgacccgt ctgcagggca
     2521 cgctgcgccg cctcggcatc aacgacacca gcccggtcgt cgtctacgac cagggcagcg
     2581 ggatggggtc ggcacgcgcc tggtggctgc tcacgtatgt ggggctggcc gacgtgcgcg
     2641 tcctcgacgg cggccttgcc gcctggacca gcgccgggta tgccgtgacc gacgctccgt
     2701 ccccgccgcc ggttcccggc ggggtcaccg tgcgacccgg ggcgctgccg gtgctcgacg
     2761 ccgcggcggc cgccgaggtg gctcggcgcg gcatactgct cgacgcccgc gccgccgagc
     2821 gcttcgcggg ccgcaccgaa cccatcgacg ccgtcgccgg acacatcccc ggcgcccgca
     2881 atgccccact cgacgagttc accggcgccg acgggcggct gctcgacaag gaacgcctgc
     2941 acgcctactt cgcgagcaag ggcgtcttcg acagcgccga ggtcggcgcc tactgcggca
     3001 gcggcatcac cgcggcgcga atggcgttgt actgcggcag atcggcg
//
LOCUS       DAOPJG010000283         2893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_39088_length_2893_cov_8.243481, whole genome shotgun sequence.
ACCESSION   DAOPJG010000283 DAOPJG010000000
VERSION     DAOPJG010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39088_length_2893_cov_8.243481"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="PKU75_16390"
     CDS             <1..1880
                     /locus_tag="PKU75_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPF82507.1"
                     /translation="NPVLIGEPGVGKTAVVEGLAQDIVRGDVPETLKDKQLYTLDLGS
                     LVAGSRYRGDFEERLKKVLKEIRTRGDIILFIDEIHTLVGAGAAEGAIDAASILKPML
                     ARGELQTIGATTLDEYRKYIEKDSALERRFQPIQVAEPTLAHAIEILKGLRDRYEAHH
                     RVTITDSALVSAATLADRYVNDRFLPDKAIDLIDEAGARLRIRRMTAPPDLREFDEKI
                     ASVRREKESAIDGQDFEKAARLRDDEKKLLSEKAHRESEWKAGDLDVVAEVDEELIAE
                     VLAASTGIPVFKLTEEESSRLLNMEGELHKRIVGMNEAIKALSQAIRRTRAGLKDPRR
                     PGGSFIFAGPTGVGKTELAKALAEFLFGDEDALIQLDMSEFSEKHTVSRLFGSPPGYV
                     GYEEGGQLTEKVRRRPFSVVLFDEVEKAHPEIFNSLLQVLEDGRLTDSQGRMVDFKNT
                     VIIMTTNLGTRDIAKGVSLGFSAGPDSRSDYERMKNKVQDELKNHFRPEFLNRVDEIV
                     VFPQLTQEEIVQIVDLEIAKLDKRLKDKDMGIELTPAAKNLLAKKGYDPVLGARPLRR
                     TIQREIEDVLSEKILYGELKAGEIVAVDADGEGKSATFTFAGTPHAQAIEIPLVIADA
                     E"
     gene            complement(2236..>2893)
                     /locus_tag="PKU75_16395"
     CDS             complement(2236..>2893)
                     /locus_tag="PKU75_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82508.1"
                     /translation="ILQQIAASISMPFAFSHCTAARILGWPVPWVAQVSGDVHVMTRT
                     PDGQIRTTGFVGHRGLESRDVSILHDLPVVAPAHTWVDLGELVGPGLPYFFEDIIGSG
                     DQALNRGCTKAELRALVESRNRPRGKRTLLPALVWMRKGSESMLETQWRLCCQRARLP
                     QADLNRNRYAPGGRFLFRPDLSWLAKRIALECQGAQFHDTPEAAAADGERMRRGSADR
                     "
BASE COUNT          537 a         1005 c          930 g          421 t
ORIGIN      
        1 acaacccggt gctgatcggc gagcccggcg tcggcaagac cgccgtcgtc gagggactgg
       61 cccaggacat cgtgcgcggc gacgtgcccg agacgctcaa ggacaagcag ctctacaccc
      121 tcgacctcgg ctcgctcgtg gccggctcgc gctaccgcgg cgacttcgag gagcggctga
      181 agaaggtcct caaggagatc cgcacccgcg gcgacatcat cctgttcatc gacgagatcc
      241 acacgctcgt cggggccggt gccgccgagg gtgccatcga cgccgcctcg atcctcaagc
      301 ccatgctcgc ccgcggcgag ttgcagacga tcggcgcgac cacgctcgac gagtaccgca
      361 agtacatcga gaaggactcg gcgctggagc gccgcttcca accgatccag gtggccgagc
      421 cgacgctggc gcacgccatc gagatcctta agggcttgcg cgaccgctac gaggcgcacc
      481 accgggtgac gatcaccgac tccgcgctgg tgtccgccgc gacgctcgcc gatcgctatg
      541 tcaacgaccg gttcctgccg gacaaggcca tcgacctgat cgacgaggcc ggggcacgct
      601 tgcgcatccg ccggatgacc gcgccgccgg acctgcgcga gttcgacgag aagatcgcct
      661 cggtgcgccg cgagaaggaa tcggccatcg acgggcagga cttcgagaag gccgcccgcc
      721 tgcgcgacga cgagaagaag ttgctctccg agaaggccca ccgcgagtcg gagtggaagg
      781 ccggcgacct cgacgtcgtc gccgaggtcg acgaggaact gatcgccgag gtgctcgctg
      841 cgtcgaccgg catcccggtc ttcaagctga ccgaggagga gtcctcacgg ctgctcaaca
      901 tggaaggcga actgcacaag cggatcgtcg gcatgaacga ggccatcaag gcgctctcgc
      961 aggccatccg gcgcacgcgc gccgggctga aggacccgcg ccgccccggc ggctcgttca
     1021 tcttcgccgg gcccacgggt gtcggcaaga ccgagctcgc caaggcgctc gccgagttcc
     1081 tcttcggtga cgaggacgcg ctcatccagc tcgacatgtc ggagttctcc gagaagcaca
     1141 ccgtctcgcg gctcttcggc tccccgcccg gctatgtcgg ctacgaagag ggcggccagc
     1201 tcaccgagaa ggtgcgccgc cggccgttct ccgtcgtgct cttcgacgag gtcgagaagg
     1261 cccaccccga gatcttcaac tcgctgttgc aggtcctcga ggacggccgc ctgaccgact
     1321 cccagggccg gatggtcgac ttcaagaaca ccgtcatcat catgacgacc aacctcggca
     1381 cccgtgacat cgccaagggc gtctcgctcg gcttctccgc cgggccggac tcgcgcagcg
     1441 actacgagcg gatgaagaac aaggtccagg acgagctgaa gaaccacttc cgtccggagt
     1501 tcttgaaccg tgtcgacgag atcgtcgtct tcccgcagtt gacgcaggag gagatcgtcc
     1561 agatcgtcga ccttgagatc gcgaagctgg acaagcggct caaggacaag gacatgggca
     1621 tcgagctcac gcccgcggcc aagaacctgc tggccaagaa gggctacgac ccggtcctcg
     1681 gtgcccgccc gctgcgccgg accatccagc gcgagatcga ggacgtgctg tccgagaaga
     1741 tcctctacgg cgagttgaag gccggcgaga tcgtcgcggt cgacgccgac ggcgagggca
     1801 agtcggcgac cttcaccttc gccggcaccc cccacgccca ggcgatcgag atccccttgg
     1861 tgatcgccga cgccgagtaa ccggcgccgc acaaccgccc agcgaaccgt atgccgcgcc
     1921 cccacccggg gggcgcggca tactgctgcc tgcccgtcct cccgccctcc ccccgtcgag
     1981 tgaacaccga aatcacgtcg agtgaacacg gaaacttcat cgagtgggaa gccacagccc
     2041 ttggcagctg aggcgcttcc gccgccggag gttctctcga agggtctcgg cggcctccgg
     2101 ggaatagggc atcggctcgg cgccgacgag atcgaggcgc ctcgcgggga actccaaact
     2161 atcggcgagt tccatcaggg cggtatcacg ggcggacttg tcccagagct gttgtgacct
     2221 gacctcgtga aaggtctacc ggtcggcgga gccacgacgc atacgctccc cgtctgcggc
     2281 cgctgcttcg ggtgtgtcgt ggaactgcgc cccctgacac tcgagcgcaa tgcgcttggc
     2341 cagccacgac aggtccggcc ggaagaggaa ccgtcctcca ggggcatagc ggttccggtt
     2401 gaggtccgcc tgcggcaggc gggcgcgctg acagcacagc cgccactggg tctccaacat
     2461 cgactcagac cccttccgca tccagaccaa ggcgggcaaa agagtgcgct taccgcgcgg
     2521 ccggtttcgc gattcgacga gggcgcgcaa ctccgccttt gtgcagccgc gattgagcgc
     2581 ctggtcgcct gatccgatga tgtcctcgaa aaaatacggc agaccgggac ccaccaactc
     2641 acccaagtcg acccaggtgt gcgccggcgc gacgacgggc agatcgtgga ggatgctgac
     2701 atcacggctc tccaaacccc ggtgcccgac gaagcccgtg gtccggatct gcccatccgg
     2761 agtcctcgtc atgacatgaa cgtccccgct cacctgcgcc acccagggca cgggccagcc
     2821 cagaatgcgc gcggccgtgc agtgggagaa ggcgaaaggc atgctgatcg acgccgcgat
     2881 ctgttgaagg atg
//
LOCUS       DAOPJG010000284         2865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_39630_length_2865_cov_7.937722, whole genome shotgun sequence.
ACCESSION   DAOPJG010000284 DAOPJG010000000
VERSION     DAOPJG010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39630_length_2865_cov_7.937722"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..581
                     /gene="aroQ"
                     /locus_tag="PKU75_16400"
     CDS             138..581
                     /gene="aroQ"
                     /locus_tag="PKU75_16400"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPF82509.1"
                     /translation="MSATIAVLNGPNLNLLGEREPGIYGSETLADVEARCRRTAESLG
                     YAVEFRQSNSEGALIDAVHELRGSAGFVVNLGAYTHTSIALRDALATVSGPIVEVHIS
                     NTHAREAFRHVSYVAPLAAAVIAGCGTLGYEFAIQVIAHRLGAKT"
     gene            940..1143
                     /locus_tag="PKU75_16405"
     CDS             940..1143
                     /locus_tag="PKU75_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPF82510.1"
                     /translation="MAQGTVKWFNAEKGFGFIAQDGGGPDVFVHYSAIDSSGYRTLDE
                     AQRVEFEVTQGPKGPQADRVRAI"
     gene            1300..2808
                     /locus_tag="PKU75_16410"
     CDS             1300..2808
                     /locus_tag="PKU75_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPF82511.1"
                     /translation="MSGPSEDHEAGTPVTRYRSRHRRPRRWRRLVAVLLSAALVVFVA
                     PTVLAYFKLNHNIKRIDISKALGSRPSTTSVSAGVDRPLNVMVMGSDTREGIGTTEYG
                     KDTVEGGAHSDTNLLVHLSADRSRALIVSIPRDSMTKAPKDCNDPKSTVANGEVRQWN
                     YNFNKGGPACTIRTLEGLTGVFVDHFIVVDFRGFQEMVDALGGVEVCTNVDIADSDAQ
                     FTLAAGRHRLTGKEALGYVRVRKTVNDGSDLNRIRRQQAFLSSVVQEATDTKLLLRPD
                     KLFAFLDAATKSMTADADLGATTLAGIANSLRHIGVDKIEFVTVPTEVYPKDPNRVQW
                     KDNASVIWEAIKADRPLPGSSDKPKPTATSTTKERLTVAPDKISVEISNDSGVEGLAT
                     QAGAALSVQGFTVAGYRNGAAGETEGVVIRHSKAQKAAARTVAAAFPGAKLKADENLG
                     SVIVVHLGMGAANPVEVPNRKGKDPLPKMTVSAPPAVPDGLDTRKADQDICS"
BASE COUNT          518 a         1005 c          882 g          460 t
ORIGIN      
        1 ccgcgcaaga tggtcaaagc ggtgaccgcg tcatctccct cgaggatgcg taaggccggc
       61 tccgacagct ccttcggcac aaccgcccgc atgtgcagca cgcctccagt gtgctccacg
      121 agcggcatac tggccgggtg agtgcgacca tcgccgtcct caacggcccc aatctcaatc
      181 tgctcggcga gcgagagccg gggatctacg gctccgagac cctcgccgac gtcgaagcgc
      241 gctgccgtcg caccgccgag agcctggggt atgccgtgga gttccgccag tccaacagcg
      301 agggcgcgct cattgatgcc gtgcacgagc tgcgtgggtc ggccggtttc gtggtcaatc
      361 tcggggcgta tacccacacg tccatcgcgt tgcgtgacgc gctggccacc gtctcagggc
      421 ccattgttga ggtgcacatt tcgaatactc acgctcgcga agccttccgg cacgtttcct
      481 atgtcgcacc cctcgcggct gcggtcatcg ccgggtgcgg gacgctcggc tacgaattcg
      541 ccatccaggt catcgctcac cggctcggtg ccaagactta ggacaaaatt ttacctaccc
      601 gtcggtagtg acttgaggtt ccggattctc tgccaaaagc gttgcgggac aacgattttc
      661 gacggcccca cgccagaacc tctggccacc tgtcaagtcc ggtccccgct gcgggcccag
      721 cacgtggtcg atccgcctgt ccttgggccg tgcggttcgt ctcggcgtgc taccgttcac
      781 gccggttgca caagcagtac tcgcataatt ggccacatcc gccccgccag ggtggcgtcg
      841 gccggggatc agaatgcccc acatgggaga aggcagggca gctacggata ttcgagcagt
      901 tcggatatct acggcaatag aaggaaagag atttacccca tggcacaggg cactgttaag
      961 tggttcaacg ctgaaaaggg cttcggtttc atcgcccagg acggcggcgg ccccgatgtg
     1021 ttcgttcact actccgcgat cgactcctcc ggctaccgca ctctcgacga ggcgcagcgg
     1081 gtcgagttcg aggtcaccca gggcccgaag ggtccgcagg cggaccgcgt tcgcgctatc
     1141 tgagactgac gcgatcggcg ggcccgggag tccgccaccg cactgtcgtc acgaacggcc
     1201 ctgaccgagc attcggtcgg ggccgttcta cgtgcccgct tcgtcttcta gactttcctt
     1261 gagaagacat acctggttcg ttccaaggaa gccccacccg tgtccggtcc atcggaagac
     1321 cacgaggccg gcacgccggt cacgcgatac cgcagtcgcc atcgtcgtcc gcggcgctgg
     1381 cgccgtctgg tcgcggtgtt gctctcggct gccttggtcg tcttcgtcgc ccccacggtt
     1441 ctggcgtact tcaagctcaa ccacaacatc aagcgcatcg acatctccaa ggcgctcggt
     1501 tcgcgcccgt cgacgacctc ggtgtccgcc ggcgtcgatc gacccctgaa cgtcatggtc
     1561 atggggtccg atacgcgcga gggcatcggc accaccgaat acggcaagga cacggtcgag
     1621 ggcggcgcgc actcggacac caacctgctc gtgcacctct ccgcagaccg cagccgggcg
     1681 ctcatcgtct cgatcccgcg ggactcgatg accaaggccc ccaaggactg caacgacccc
     1741 aagagcaccg tggccaatgg ggaggtccgc cagtggaact acaacttcaa caagggcggc
     1801 ccggcctgca cgatccggac cctcgaaggc ctgaccgggg tcttcgtcga ccacttcatc
     1861 gtcgtcgact tccgcggatt ccaggagatg gtcgacgccc tcgggggagt ggaggtgtgc
     1921 accaatgtcg acatcgccga ctcggacgcg cagttcacgc tcgcggcggg ccgacaccgc
     1981 ctgaccggga aggaagccct cggctacgtc cgcgtgcgca agaccgtcaa cgacggcagt
     2041 gacctgaacc ggattcgccg ccagcaggcc ttcttgtcct cggtcgtcca ggaggcgacc
     2101 gacaccaagc tgctgcttcg cccggacaag ctgttcgcct tcctcgacgc cgccaccaaa
     2161 tcgatgacgg ccgacgccga cctcggcgcc accacgctgg cagggatcgc caacagcctg
     2221 cgccacatcg gggtcgacaa gatcgagttc gtcaccgtgc ccaccgaggt ctacccgaag
     2281 gacccgaacc gggtgcagtg gaaggacaac gcatcggtga tctgggaggc catcaaggcc
     2341 gaccggcccc tgccgggttc gagcgacaag ccgaagccga ccgccaccag cacgacgaag
     2401 gagcgcctga cggtcgcccc ggacaagatc tccgtcgaga tcagcaacga ctccggcgtc
     2461 gaaggcctcg ccacgcaggc cggcgccgcg ctcagtgtcc agggcttcac tgttgccggc
     2521 tatcgcaatg gcgcggccgg cgagacggag ggcgtcgtca tccggcattc caaggcccag
     2581 aaggcggccg cccgcaccgt cgcagcggcc tttcccggcg ccaaactcaa ggcggacgaa
     2641 aacctcggct cggtgatcgt ggtgcacctg ggcatgggcg ccgccaaccc cgtcgaagtc
     2701 ccgaaccgca agggcaagga cccgctcccg aagatgaccg tctccgcgcc acccgcggtg
     2761 cccgacggcc tcgacacccg caaagccgac caggacatct gcagttgagc gggcggcata
     2821 ccggcccctg acgagaaccc gacgaagctc ggcgcacgcc gcccg
//
LOCUS       DAOPJG010000285         2775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_41454_length_2775_cov_7.981618, whole genome shotgun sequence.
ACCESSION   DAOPJG010000285 DAOPJG010000000
VERSION     DAOPJG010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41454_length_2775_cov_7.981618"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..827
                     /locus_tag="PKU75_16415"
     CDS             249..827
                     /locus_tag="PKU75_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HPF82512.1"
                     /translation="MSESTNNGTKTIAFLVAAEGIEQVELTDPWQAVVDAGHTAVLVS
                     PKSGTVQMFNHLDKADTQKVDKTVADVSADDFDALVLPGGVANPDALRTDKDAVALVK
                     DFMASGKAVAAICHAPWTLVEADVVRGRKLTSWPSLATDIRNAGGEWVDERSVVDGNL
                     ITSRKPDDLPAFISALLDAVEHGAAATGQESA"
     gene            824..1813
                     /locus_tag="PKU75_16420"
     CDS             824..1813
                     /locus_tag="PKU75_16420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPF82513.1"
                     /translation="MTRFGYTLMTEQSGPKELVAYAEKAEHVGFDFEVMSDHYFPWLS
                     AQGHAPYAWSVLGAVAHVTNRVELYTYVTCPTMRYHPAVVAQKAATIQLLADGRFTLG
                     LGSGENLNEHVIGEGWPAVDARQQMLAEAIEIIRALHTGELVTHDGEYFRVDSARIWD
                     LPDGGIPIGVAAGGPEAIEAFGALGDHLIATEPSADLIAAWNNQEGTKRIGKGAKAIG
                     QIPISWDPSGKKAAVARAHEQFRWFAGGWAVNADLPTTAGFAGASKFVREEDVAESIP
                     CGDDLDAIVEAVRPYWEAGFTDIALVQIGDELQERFLNEAAEPLLEKLRAAAG"
     gene            complement(1830..>2775)
                     /locus_tag="PKU75_16425"
     CDS             complement(1830..>2775)
                     /locus_tag="PKU75_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82514.1"
                     /translation="FDLGDQWTHLCTVEGTGDPETVYGIVPNLPVPVWGWGAIPDQYG
                     RRWADDTGDDDEAPEPRHPNPLTDPWLATTGPTPVVDMGAVCAATDGPALIDALTGVE
                     LDSALQQIGDHLLRTFAVTPSRQRGSLQSVMLSIHGRLMMRGWEGDEALAEELLAQLD
                     GNHRPGRPVPVNLDDLDIALSDHSAEYPGGYLNTDTGEVVPAFMAHGGYVDEPIDVED
                     DAWLYVEPVSSRTGWEDMETFAESVSDPTLRDLLTRAIAGKGAFRRFKDVLHQDSDDA
                     TRTRWRLEEQDRSLGRARALLASHGIRSVARTGPTVVG"
BASE COUNT          453 a          940 c          973 g          409 t
ORIGIN      
        1 gaatccaaga gatttcccaa ggcactccac cgccacgtcg gcgacgatct ggtcaccggg
       61 cccgaacaac cccgagccgc gagggggtga tcggtgtcgc cgttcgtcga agcagccggg
      121 cctgccgagc cggctcgccc gaaagggtgg tttcgatggc catggggccg caatgcgccg
      181 ttggcgcggc ccgcggccta gcgtcgtagg aggacttctc ggcccaccaa caacaacagg
      241 agcacaccgt gagcgaatcg accaacaacg gcaccaagac catcgccttc ctcgtcgccg
      301 ccgaaggcat cgagcaggtg gaactcaccg acccgtggca ggccgtggtc gacgccggac
      361 acacggccgt cctcgtcagc cccaagagcg gcacggtgca gatgttcaac cacctcgaca
      421 aggccgacac ccagaaggtg gacaagacgg tcgccgacgt cagcgccgac gacttcgacg
      481 cgctcgtcct gccggggggc gtggcgaacc ccgacgcgct gcgcaccgac aaggacgcgg
      541 tcgccctcgt gaaggacttc atggcctcgg gcaaggccgt cgccgcgatc tgccacgcgc
      601 cgtggacctt ggtggaggcc gatgtcgtgc gcggccgcaa gctcacctcg tggccgagcc
      661 tggcgaccga catccgcaac gccggcggcg agtgggtgga cgagcgctcc gtcgtcgacg
      721 gcaacctgat caccagccgc aagccggacg acctgcccgc cttcatctcg gcgctgctgg
      781 atgccgtcga gcacggggcc gcggccaccg gccaggagag cgcatgacgc gcttcggcta
      841 caccttgatg accgagcagt ccggtccgaa agagcttgtg gcgtatgccg aaaaggccga
      901 gcatgtcggc ttcgacttcg aggtgatgag cgatcactac ttcccgtggc tgagtgcgca
      961 aggccatgcg ccgtatgcct ggagcgtcct cggcgctgtc gcgcacgtga cgaaccgggt
     1021 cgagctctat acctatgtca cctgtccgac gatgcgctac cacccggcgg tcgtcgcgca
     1081 gaaggcggcg accatccagc tgctcgccga cggccgattc accctcggcc tcggctccgg
     1141 agagaacctc aacgagcacg tgatcggcga gggctggccg gcggtcgatg cccgccagca
     1201 gatgctggcc gaggcgatcg agatcatccg cgcactgcat accggcgagc tggtcaccca
     1261 cgacggggag tacttccgcg tcgactcggc gcggatctgg gatctgcccg acggcggcat
     1321 acccatcggg gtcgccgccg gcgggcccga ggccatcgag gcgttcggcg cgctcggcga
     1381 tcacctgatc gcgaccgagc cgtcggccga cctcatcgcc gcgtggaaca accaagaggg
     1441 tacgaagcga atcggcaagg gcgccaaggc aatcgggcag atcccgatct cgtgggatcc
     1501 gtcgggcaag aaggcggccg tggcccgggc gcacgagcag ttccgctggt tcgccggtgg
     1561 ctgggcggtc aatgccgacc tgccgacgac ggcgggtttc gccggcgcgt cgaagttcgt
     1621 gcgcgaggag gatgtcgccg agtccatccc ttgcggcgac gacctggacg ccatcgtgga
     1681 ggccgtgcgc ccttactggg aggccggctt caccgatatc gcgctcgtcc agatcggcga
     1741 cgagttgcag gagcgcttcc tgaacgaggc ggccgagccg ctgttggaga agctgcgcgc
     1801 cgccgccggc tgaccggtgg ggtcggcggt cagccgacca ctgtgggccc ggtccgcgcc
     1861 accgaccgta tgccgtggct cgccagcagc gcgcgggcgc ggccgaggct gcggtcctgt
     1921 tcttccaggc gccaccgggt gcgggtggca tcgtcggagt cctgatgcag gacgtccttg
     1981 aaacgccgga aggcgccctt gccggcgatc gcgcgggtga gcaggtcgcg cagcgtcggg
     2041 tcggacaccg attcggcgaa ggtctccatg tcctcccagc cggtgcggct gctcaccggc
     2101 tcgacgtaga gccacgcgtc atcctcgacg tcgatgggct cgtcgacata cccaccatga
     2161 gccatgaagg ccgggaccac ctctccagtg tcggtgttga ggtaaccacc cgggtattcc
     2221 gcactgtggt ccgagagagc gatgtcgagg tcgtcgaggt tgaccgggac gggccgacct
     2281 ggacggtgat tgccgtcgag ttgcgcgagg agttcctcgg caagcgcctc gtcgccctcc
     2341 cagcctcgca tcatcaatcg gccgtggatc gagagcatga ccgactgaag gctcccccgc
     2401 tggcgggacg gggtcaccgc gaacgtgcgg agcaggtgat caccgatctg ctgcagcgcg
     2461 gagtcgagtt ccactccggt gagcgcgtcg atcaaggccg gcccgtcggt cgcggcgcag
     2521 acggccccca tgtcgaccac cggcgtcggc ccggtggtgg ccagccacgg atcggtgagg
     2581 gggttggggt gccggggctc gggtgcctcg tcatcgtcgc ccgtgtcgtc ggcccaccgc
     2641 cgaccgtact ggtcggggat ggcgccccaa ccccacacgg ggacagggag attcggcacg
     2701 atgccataga cagtctcggg gtcgccggtg ccctcgacgg tgcacaggtg ggtccactgg
     2761 tcgccgaggt cgaag
//
LOCUS       DAOPJG010000286         2738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_42299_length_2738_cov_6.749161, whole genome shotgun sequence.
ACCESSION   DAOPJG010000286 DAOPJG010000000
VERSION     DAOPJG010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42299_length_2738_cov_6.749161"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1262
                     /locus_tag="PKU75_16430"
     CDS             3..1262
                     /locus_tag="PKU75_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPF82515.1"
                     /translation="MRLDRRLWSTIVLVGLVGQLAWTVENMYLNVFIYETITDNPTVI
                     ATVVAASAITAALTTLFIGPLSDRLGRRRPFISGGYLLWGLATAAFGLIAAGGPLGLS
                     VGAAIVAVIALDCVMTFFGSTANDAAYQAWVTDVTTPAVRGRVDAVLQTLPLIAMLLV
                     FVALDPLTKDGQWLTFFAVVGVIVTVVGVAAHTLVRDVAAPSPPGESYAASVVSGIRP
                     SAVRANPLLYLTLATWALWGISTQVFMPYLLIYLEHYLHLDAYALVLGGALIIASAIT
                     ILSGRLIDRFGKARVLIPTLLLYAVGLLLFAPARGTVPTLLAGAVVLTGLMLTAAALA
                     AITRDRTPADRAGQVQGVRMLLVVMLPMVTGPFLGAAVIAGNDATYTDLGTARTVPTP
                     AIFVAAAAVVALALIPALRLRRALESA"
     gene            1259..>2738
                     /locus_tag="PKU75_16435"
     CDS             1259..>2738
                     /locus_tag="PKU75_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPF82516.1"
                     /translation="MIPLPEYPRPQLVREGWLNLNGSWDCAITDGDPPAAYDGTILVP
                     FSPEAPLSGVDRSLQPHETLWYRRVISRESLPPGPDGSRLLLHFGAVDQQVVVHANGT
                     QVADHVGGYLPVTADLTAIGGDIELVVQVTDASDTGWHTRGKQRIDRGGIWYTPQSGI
                     WQTVWIERVPAGGVERLVTTPYHANGMWGLEVTVESDSATAEVEIVGSGRRTIPCGEA
                     HRIPIPEPRLWSPEHPHLYDLAITCGGETVTSYAGLRTVGIGADDDVPRLLLNDEPYF
                     HAGILDQGYWPDGLYTAPSDEALRADVVAAKEFGFTMIRKHVKVEPLRWYYHCDREGI
                     LVWQDMPNGGRSYRSTVISAPAVTPIRLRDNRYAAFGRQDPAGRTQFRAELREMIEHL
                     RSLTSIVCWVPFNEGWGQFDANEIADEVHRLDPTRHVDHASGWHDQGGGDIRSLHVYF
                     RRFRMPRRTDNRPVALSEYGGYNLRVAGHTWGEKDFGYRTASD"
BASE COUNT          409 a         1019 c          862 g          448 t
ORIGIN      
        1 ccatgcggct cgatcgacgg ctctggagca ccatcgtcct ggtcggtctc gtcgggcagc
       61 tcgcgtggac ggtcgagaac atgtacctca acgtcttcat ctacgagacc atcaccgaca
      121 atcccaccgt catcgcgaca gtcgtggcgg caagcgccat aaccgctgct ctcacaacgc
      181 ttttcatcgg gccgctctcg gatcgactcg gtcgccgccg gccgttcatc tcgggcggtt
      241 acctcctgtg gggcctggcc actgccgcgt tcggcctcat cgccgcgggc ggccccttgg
      301 gcctgtccgt gggggccgcg atcgtcgcgg tgatcgccct cgactgtgtg atgaccttct
      361 tcggctccac cgccaatgac gcggcatacc aagcctgggt cacggacgtg acgacccccg
      421 ccgtgcgcgg ccgggtggac gccgtcctcc agacactgcc gctcatcgcc atgctcctgg
      481 tcttcgtcgc cctggatccg ctcaccaagg acggccagtg gctcaccttc ttcgcagtcg
      541 tcggcgtgat cgtcaccgtg gtcggcgtgg cggcgcacac cctcgtccgg gacgtcgccg
      601 cacccagccc gcccggcgag tcgtatgccg cgagcgtggt gagcggcata cggccgagcg
      661 cggtgcgcgc caatcctctg ctctacctca ccctcgcgac atgggcgctg tgggggatct
      721 cgacccaggt cttcatgccc tacctgttga tctacctcga gcattatctg cacctcgacg
      781 cctacgccct cgtgctcggc ggggcgctga tcatcgcgag cgcgatcacg atcctgtccg
      841 gccgcctgat cgaccggttc ggcaaggccc gcgtgctgat ccccaccttg ctgctgtatg
      901 ccgtgggcct cctcctcttc gcccccgccc ggggaaccgt tcccaccctg ctcgccgggg
      961 ccgtcgtgtt gacgggcctg atgctcaccg ccgcggcctt ggccgcgatc acccgggacc
     1021 ggacgccggc cgatcgggcc ggccaggtcc agggagtgcg gatgctgctg gtcgtcatgc
     1081 tcccgatggt gaccggcccc ttcctcggcg ccgcggtcat cgccggcaac gacgcgacct
     1141 ataccgacct cggcaccgcg cggaccgtgc cgacgccggc catcttcgtc gccgccgcgg
     1201 ccgtcgtcgc tctcgctctc atccccgccc tccgacttcg ccgcgccctg gagtcggcat
     1261 gatcccccta ccggaatacc cacggccgca actggttcga gaaggctggc tcaacctgaa
     1321 cgggtcatgg gactgcgcca tcacggacgg ggacccgccc gcggcctatg acgggacgat
     1381 cctcgtgccc ttctcccctg aggcgccctt gtcgggggtc gaccgcagcc tgcagccgca
     1441 cgagaccctc tggtatcggc gcgtgatcag ccgggagtcc ctgccacccg gtcccgacgg
     1501 cagtcgcctc ctgctgcatt tcggggcggt cgaccagcag gtcgtcgtcc acgccaacgg
     1561 cacgcaggtg gccgaccacg tcggcggcta tctgcccgtc accgcggacc tgacagccat
     1621 cggcggcgac atcgaactcg tcgtccaggt caccgacgcc agcgacaccg gctggcacac
     1681 gcgcggcaag caacggatcg accggggcgg catctggtac accccccagt ccggcatctg
     1741 gcagaccgtc tggatcgaac gcgtcccagc cggcggtgta gagcgcctgg tcaccacgcc
     1801 ctaccacgcg aacggcatgt ggggtcttga ggtgactgtc gaatctgact ctgccacagc
     1861 ggaagtcgaa atcgtcggca gcggtcgacg gacgatcccc tgcggggagg cacaccgcat
     1921 acccatcccc gagccgcggc tctggtcgcc cgagcatccc cacctctacg acctcgcgat
     1981 cacctgcggc ggcgagacgg tgacctccta cgcgggactg cggaccgtcg ggatcggcgc
     2041 cgacgacgat gtaccccgcc tcctgctcaa cgacgagccc tacttccacg cgggcatact
     2101 cgatcagggc tattggccgg atgggctcta cacggcgccg agcgacgagg ccctgcgcgc
     2161 ggacgtggtg gcggccaagg agttcggctt cacgatgatc cgcaagcacg tcaaggtcga
     2221 gccgctgcgc tggtattacc actgcgaccg cgagggcatc ctcgtctggc aggacatgcc
     2281 gaacggcgga cgcagctacc gaagcacggt catcagcgcc cccgctgtga ctccgattcg
     2341 cctgcgcgac aaccggtatg ccgccttcgg ccgccaggat cccgccggaa ggacacagtt
     2401 ccgggccgaa ctgagggaga tgatcgagca cctgcgatcc ctcacctcga tcgtgtgctg
     2461 ggtgccgttc aacgagggct ggggccagtt cgacgcgaac gagatcgccg acgaggtgca
     2521 ccgtctcgac cccacccgcc atgtcgacca cgcgtcgggc tggcacgacc agggcggcgg
     2581 ggacatccgc tccctgcacg tctacttccg gcgtttccgg atgccgcgcc gcaccgacaa
     2641 ccggccggtc gcgctgagcg aatacggcgg ctacaacctg cgcgtggccg gccacacgtg
     2701 gggagagaag gacttcggct atcgcacggc gagcgatg
//
LOCUS       DAOPJG010000287         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_44049_length_2659_cov_8.192012, whole genome shotgun sequence.
ACCESSION   DAOPJG010000287 DAOPJG010000000
VERSION     DAOPJG010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44049_length_2659_cov_8.192012"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKU75_16440"
     CDS             complement(<1..280)
                     /locus_tag="PKU75_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82517.1"
                     /translation="MTTTPDAAAPVVRAAYVQRPQTETFAIFTDQIGAWWPLPTHGVF
                     GEQAGGLEFRDGRLIELAVDGRESTWGEVRAWEPPSRMVISWHPGRDTG"
     gene            complement(277..597)
                     /locus_tag="PKU75_16445"
     CDS             complement(277..597)
                     /locus_tag="PKU75_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPF82518.1"
                     /translation="MAYGSAAMDALGDPTRRRILEVLRDGEHSVRELTDATDVSQPAV
                     SQHLRVLKGAGLVEVRPAGARRLYSVNPGGLADVRAWVDSFWDDALDAFVQHANSTPP
                     GRTP"
     gene            663..1763
                     /locus_tag="PKU75_16450"
     CDS             663..1763
                     /locus_tag="PKU75_16450"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPF82519.1"
                     /translation="MSEFVVSPEVAAAPKALLHDHLDGGLRPQTIIDIADEIGYRALP
                     ATDPADLDRWFRESADSGSLVRYLETFAHTVAVMQTEAGLYRVAKECALDLAADGVVY
                     AESRYAPEQHVDGGLTMEAVVEAVNAGFRDGESEAAAAGNTIQVRALLTAMRHAARAR
                     EIAELTVRYRDAGVVGFDIAGAEAGYPPTRHLDAFEYLQRENSRFTIHAGEAFGLPSI
                     WEAIQWCGADRLGHGVRIVDDIDTSGEVPTLGRLSAYVKDGRIPLEMCPTSNLQTGAA
                     KSYAAHPITLLKDLRFRVTVNTDNRLMSGTTQGREMQHLVDEAGWTLADLRWATINAM
                     KSAFIPYDERLAIINDQVKPGYAALGVVSYVP"
     gene            1878..2270
                     /locus_tag="PKU75_16455"
     CDS             1878..2270
                     /locus_tag="PKU75_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82520.1"
                     /translation="MISLHDETGGLTTPARLAERSGLSHYDVEAALRALAREQPVLFE
                     TQGATTLGRFLKVGNPTGRARRICGAWPTPEQVTQKLLSAIRDAADTEADAQLQAALR
                     LVSAYFHGPGRCRAAAIGAAAIAWDREW"
     gene            complement(2333..>2659)
                     /locus_tag="PKU75_16460"
     CDS             complement(2333..>2659)
                     /locus_tag="PKU75_16460"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HPF82521.1"
                     /translation="GWSVLTNPWQHIVVPVLAVGVWAIWGPRGWVSLRLVPWALLIPV
                     GWIAWVLLRGLAVAAYPYAFVDARTHGYARVFTTIGAILVFALAVAALYWAIDVLLRR
                     RRTPHP"
BASE COUNT          443 a          894 c          934 g          388 t
ORIGIN      
        1 cgccggtgtc ccggcccgga tgccaggaga tcaccattct gctcggcggt tcccacgcac
       61 ggacctcgcc ccacgtgctt tcccggccgt caacggcgag ttcgatcagc cggccgtcgc
      121 ggaactccag cccgccggcc tgctcgccga agacgccatg ggtgggcagc ggccaccatg
      181 caccgatctg gtcggtgaag atggcgaagg tctccgtttg gggacgctgg acgtatgccg
      241 cgcgcaccac cggtgctgcc gcgtcgggag tggtggtcat ggggtcctcc ccggaggtgt
      301 cgaattggcg tgctggacaa aggcatcgag ggcgtcatcc cagaaggagt cgacccaggc
      361 ccggacgtcg gcgaggccgc cggggttgac gctgtagagg cggcgcgcgc ccgcgggtcg
      421 gacctccacc aggcccgcgc ctttgaggac gcgaaggtgc tgggagacgg ccggctgact
      481 cacgtccgtg gcatcggtca gctcgcgcac gctgtgctcg ccgtcgcgca acacctcgag
      541 gatgcgccga cgggtggggt cgcccaaggc atccatcgcc gcgcttccat aagccatggc
      601 taatgataag cagacactta tgcacgagcg caagggcccc gggcacggca taccctgagc
      661 ccgtgagtga attcgtcgtc agcccggaag tcgccgccgc cccgaaggcg ctgctgcacg
      721 atcacctgga cggggggctg cggccgcaga cgatcatcga catcgccgac gagatcggct
      781 atcgcgcgct gcccgccacc gaccccgcgg atctcgaccg gtggttccga gagagtgcag
      841 actccggctc cttggtgcgc tatctcgaga cgttcgccca caccgtggcc gtcatgcaga
      901 ccgaggccgg gctctatcgg gtggccaagg agtgcgccct cgacctcgcg gccgacggcg
      961 tcgtctatgc cgaatcccgt tacgccccag agcaacacgt cgacggtggc ctgacgatgg
     1021 aggcggtggt cgaggccgtc aatgccggct tccgcgacgg ggagtccgag gcggccgcgg
     1081 ccggcaacac catccaggtg cgtgctctcc tcaccgccat gcggcatgcc gcccgcgctc
     1141 gcgagatcgc cgagctgacc gtgcgatacc gcgacgccgg tgtcgtcggg ttcgacatcg
     1201 ccggcgccga ggcgggctac ccgcccaccc gacacctcga cgccttcgag tatctgcagc
     1261 gcgagaactc ccgcttcacc atccacgccg gcgaggcctt cgggctgccg agcatctggg
     1321 aggcgatcca gtggtgcggg gccgaccggc tcggccacgg cgtacgcatc gtcgacgaca
     1381 tcgacacgtc cggtgaggtg ccgacgctcg gccggctctc ggcatacgtc aaggacggcc
     1441 gcatcccgct cgagatgtgc ccgaccagca atctgcagac cggggccgcg aaatcctatg
     1501 cggcacaccc gatcacgctc ctgaaggacc tgcgattccg ggtcacggtc aacacggaca
     1561 accggctgat gagcggcacc acccagggtc gggagatgca gcacctggtc gacgaggccg
     1621 ggtggacgct cgccgacctg cggtgggcca cgatcaatgc gatgaagtcc gcgttcatcc
     1681 cctatgacga gcggctggcc atcatcaacg atcaggtcaa gccggggtat gccgcgctgg
     1741 gcgtcgtctc gtatgtcccg taagggccct ctgcccgctc gtgtcatcga cgtgagggtc
     1801 tggtcaccgg agcgcagacg cgcttgagtg aaagggtgaa agagacctgg gatcgagaca
     1861 tcagtgtttt gcgagcgatg atctcgctgc acgacgagac cggtgggctc acgacgccgg
     1921 ctcgactcgc cgagcggtcc gggttatcgc actacgacgt cgaggcggcg cttcgagcgc
     1981 tggcacgaga acagccggtc ctcttcgaga cccagggcgc gacgacgctc ggacgattcc
     2041 tgaaggtcgg gaaccctacc gggcgggcgc gcaggatctg tggcgcgtgg ccgacgccgg
     2101 agcaggtgac gcagaagctg ctctcggcca tccgcgacgc cgctgacacc gaggccgatg
     2161 cgcagttgca ggcggccctg cggctggtgt ctgcgtattt ccacggtccg ggccgttgtc
     2221 gtgctgccgc gatcggggct gcggctatcg cgtgggatcg tgagtggtga gttctcgaaa
     2281 ctggcccctc gggcgcgggt gagtcagttg cgcagcaggc ggttagcacc gctcaggggt
     2341 gcggggtgcg ccggcgacgc aagagcacat cgatggccca gtacagcgca gcgacggcga
     2401 gcgcgaagac caggatggcg ccgatggtcg tgaagacacg ggcatacccg tgggtgcggg
     2461 cgtcgacgaa ggcatacggg taggcggcga cggcgagtcc gcgcaggagg acccacgcga
     2521 tccagccgac ggggatcagc agtgcccacg ggaccaggcg cagcgagacc caaccgcgcg
     2581 ggccccagat cgcccagacc ccgacggcca ggaccggcac gacgatgtgc tgccacgggt
     2641 tggtcaggac cgaccaccc
//
LOCUS       DAOPJG010000288         2593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_45774_length_2593_cov_3.901497, whole genome shotgun sequence.
ACCESSION   DAOPJG010000288 DAOPJG010000000
VERSION     DAOPJG010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45774_length_2593_cov_3.901497"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..2202)
                     /locus_tag="PKU75_16465"
     CDS             complement(133..2202)
                     /locus_tag="PKU75_16465"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPF82522.1"
                     /translation="MRIRVEHRDDAIGIGERRPRLSWQLPAPDAGRQHAYRLRLDDEL
                     SPWVESDGTVLQPWPFDPLHSGEQHEIAVQVRTDMGDSEFSEPVALEAGLLNVADWQA
                     SWVSPTPREPETPGFRPAYRLRGVTTVDRPVARARLYVTAHGIHETFLNGEPVSDEVL
                     APGYTEYAARLHVSTIDVTTRIRLGDNAVDVLLADGWFRGSVGMPRSTDQFGNRTAYL
                     AQLHLTFEDASTVVTGTGASWRWAPSHVVAADLIEGQQQDNRLRQSGWTATDLDAPSW
                     EPVTIREDIGYAALTASPAPPVRRITELAAVAATRPRPGTYVLDFGQNIGGWTRLDDL
                     TALGPAGTTLTLTHAEAVDAAGDVMTDHLRPDDIPFIQHEVRAGMVDRVTIAGELGES
                     FEPTLTSHGFRYVRLVGVSHDLAPEEVAARARAVVVHTDLPERGSFACSNADLNALHA
                     AAVRSFRGNAISIPTDCPTRERAGWTGDWQIYAPVASFHHDIAGFSLSWLRDLAAAQW
                     DNGIVGNMAPMPPAERSGFLEKLNGSAGWGDAIVLVPWELYAEYGDLRSLAEMWPNMQ
                     RWLAFAAASAATGRHPSRVARSPAPLDHETWLWDTGFHWGEWLVPGEEIDFPAFLAAD
                     KADVATAYLVGQPRPWPPSPRCWADRRTRGTVATSPTRPAPPGSRSSSPRTAPSRPTS
                     RPTSCGR"
     gene            complement(2298..>2593)
                     /locus_tag="PKU75_16470"
     CDS             complement(2298..>2593)
                     /locus_tag="PKU75_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82523.1"
                     /translation="AEAAGIADRLVAVRVTSLLTVPFAIKGTNMCAFVPSRLAHRVLE
                     SLDLAIARTPLTQVQITEAAHWHQRRDNDPAVTWLRHLLYDVAIELEDAAPSE"
BASE COUNT          399 a          834 c          962 g          398 t
ORIGIN      
        1 tgtcggcgag agcgggcagg aggtcacggg tggcaaggaa gcccgtgccg agccgggtgc
       61 cgttgtcgcg gaccaggcga tccaattgcg cggcagcggt tttgcgcagg tgatcgggta
      121 cgaggccgaa cgtcagcgcc cgcacgaggt tggcctgctt gtcgggcgtg atggtgccgt
      181 ccgcggcgac gaactcctgc tgccaggcgg tgcgggcctt gtcggcgagg tcgcgacagt
      241 gccgcgcgtc cgccggtcgg cccagcacct cggcgatggc ggccatggtc tcggctgacc
      301 gaccagatag gcggtggcga cgtcggcctt gtccgcggcg aggaaggcgg ggaagtcgat
      361 ctcctcgccc ggcacaagcc attctcccca atggaaaccg gtgtcccaca gccatgtctc
      421 gtgatcgagc ggcgcggggg agcgggcgac ccgcgacggg tgacgcccgg tcgcggcgct
      481 cgctgccgcg aaggcgagcc agcgctgcat gttcggccac atctcggcga ggcttcgcag
      541 gtcgccgtac tcggcataca gttcccacgg cacgaggacg atggcgtcgc cccagccggc
      601 cgagccgttg agcttctcca ggaagcccga tcgctcggcg gggggcatcg gcgccatatt
      661 gccgacgatg ccgttatccc attgcgccgc agcgagatcg cgcaaccacg acagggagaa
      721 gccggcgatg tcgtggtgga agctggccac gggtgcatag atctgccagt caccggtcca
      781 cccggcccgc tccctggtcg ggcagtcggt cgggatggag atggcgttgc cacggaaact
      841 ccggacggcg gcggcgtgca aggcgttgag gtcggcgttg ctgcacgcga acgacccgcg
      901 ctcgggcagg tccgtatgga cgacgacggc gcgggcccgg gcggccacct cctccggcgc
      961 gagatcgtgg ctgacgccca cgaggcgcac gtagcggaag ccgtggctgg tcagggtcgg
     1021 ctcgaagctc tcacccagtt ccccggcgat ggtgacccga tcgaccatcc cggcgcgcac
     1081 ctcgtgctgg atgaaaggaa tgtcatcggg ccgcaggtgg tcggtcatca cgtcaccggc
     1141 ggcgtcgacg gcctccgcgt gggtcagcgt gagcgtggtg ccggccgggc cgagtgccgt
     1201 gaggtcgtcc agtcgcgtcc atccgccgat gttctgcccg aagtcgagga cgtaggtgcc
     1261 cggccgtggg cgcgtcgcgg cgactgcggc gagttcggtg atgcggcgca ccgggggtgc
     1321 cggcgaggcg gtgagcgcgg catacccgat gtcttcgcgg atcgtgaccg gctcccagga
     1381 aggggcgtcg agatctgttg cagtccaacc cgattgacgg agccggttgt cttgctgctg
     1441 accctcgatg aggtcggccg cgacgacatg tgacggcgcc cagcgccacg aggcgccggt
     1501 gccggtgacg accgtcgaag cgtcctcgaa cgtgaggtga agttgcgcga ggtatgcggt
     1561 gcggttgccg aactgatcgg tcgagcgcgg catacccacc gacccgcgaa accagccgtc
     1621 ggcgagaagg acatcgacgg cattgtcgcc cagtcgaatc cgagtggtga cgtcgatggt
     1681 ggacacgtgc agccgggcgg cgtactccgt atagccaggc gccagcacct cgtcgctcac
     1741 cggctcgccg ttgagaaagg tctcgtggat gccgtgagcg gtgacataca gccgggcgcg
     1801 cgcgaccggc cggtccactg tcgtgacacc gcgcaggcgg taggcgggcc ggaagcccgg
     1861 agtctccggc tcacgtgggg tgggcgacac ccaggacgcc tgccagtcag caacattcag
     1921 cagcccggct tcgagggcga ccggttcgct gaactcgctg tcccccatat cggttcgcac
     1981 ttggaccgcg atctcgtgct gctcccccga atgcaatgga tcgaagggcc acggttgcag
     2041 gacggtgccg tcgctctcga cccatggact cagttcgtcg tcgagacgga ggcggtaggc
     2101 atgctggcgc ccggcatcgg gggcgggtag ttgccaggac agccgcggcc gccgctcccc
     2161 gatcccgatg gcgtcgtcgc gatgctcgac ccggatgcgc atccgcctag ccgaccagga
     2221 gatcgcgggt gggggaaatc gcgcctcggc atggcaccga ttcccctcct cgatggcgag
     2281 gagcggacaa tcgcatgcta ttcgctcggg gcagcgtcct cgagttcgat cgccacgtcg
     2341 tagagcaggt gccgcaacca cgtcaccgct gggtcgttgt cgcgccgctg atgccagtgc
     2401 gccgcctcgg tgatctgcac ctgagtgagc ggggttcggg cgatcgcgag atcgagcgac
     2461 tcgaggacgc ggtgggcgag ccgggagggc acgaaggcgc acatgttcgt gcccttgatg
     2521 gcgaagggaa ccgtcagcag actggtgaca cgcacggcga caaggcggtc ggcgatcccg
     2581 gccgcctcgg cgg
//
LOCUS       DAOPJG010000289         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_46267_length_2572_cov_6.764005, whole genome shotgun sequence.
ACCESSION   DAOPJG010000289 DAOPJG010000000
VERSION     DAOPJG010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46267_length_2572_cov_6.764005"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1259
                     /locus_tag="PKU75_16475"
     CDS             243..1259
                     /locus_tag="PKU75_16475"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPF82524.1"
                     /translation="MTTMAGRATIREVADAAGVSRQTVSNSLIHPERVKGPTLERVRR
                     VIDELGYRPSNAAQSLRSQRTGAVGFELNATGSATRNAVSFPFVLALSERAPAHNGHI
                     VTFGTTSEHPLLSGYEDMVRSQLVDAFILADTHEGDPRPDWLDGARIPYAAFGRLWGR
                     PELTSWADVDGAHGLRIATEHLLAQGYSTVGFLGWPAGSQVGDDRRRGWAAVHEDRSM
                     PLGPEAMAVQDLGAATAAAGPLLDQVGRGGAIACASDMFALGVERAAAARGWRSGTDI
                     GICGFDASVIAEIESLTSVALPLYEIADHLLGLLQDRLGGAPPPAAGLLVEPGLTIRS
                     SSTR"
     gene            1489..2427
                     /locus_tag="PKU75_16480"
     CDS             1489..2427
                     /locus_tag="PKU75_16480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPF82525.1"
                     /translation="MGLRFGFGADRRCPEDAHRLLRRRETAAVKAAADVTYDGKQYCV
                     PKDFSSLALQINTDMWEKAGLTDADIPTTWDQLTEVSKKLTSGKVAGLGIGVGIDRLG
                     AFVVQNGGWWLNADNTAGAAADAKVTDALKYVQDNVKAGNFQMSNDLGAGWGGEAFGK
                     QLAAMVIEGNWIKGAMKNDFPDVKYQTVELPAGAQKGTLMFTQCWGLAADSQNKDAAK
                     DLIKSLTTAEQQIAFADAFGVMPSRTDAAETYKEKYPEDAAFVAGGEYGHGPINLPDM
                     TEVVADLNSQLEKMSTADIAGATTAFDENAASAIGG"
BASE COUNT          424 a          952 c          819 g          377 t
ORIGIN      
        1 gtggcattgc cgtagacggc gatctgccgg tcgtgcagcc agggctggcg gcctttgtcg
       61 ctggtcacag tccctcttct tctacgcgga atcgcggctc acggcttgac aggggcccgg
      121 atggggtccc cagttgccac aacttgatcg ttcaaactca ccctagtcga tcacgatcag
      181 tggtaagaat tgaacgttca cagcagccta gcggttgaga aaagggagac gaggtgaagg
      241 gcatgaccac gatggcggga cgggcgacga tccgcgaagt cgccgatgcg gcaggcgtgt
      301 cacgccagac ggtgagcaac tccctcatcc accccgagcg cgtcaaggga cccacgctgg
      361 agcgcgtgcg ccgcgtcatc gacgaactcg gataccggcc ctcgaatgcc gcgcagagcc
      421 tgcgctcgca gcgcaccggc gccgtgggct tcgaactcaa cgcgaccgga tcggccaccc
      481 gcaatgccgt cagctttccc ttcgtgctcg ccctgagtga gcgcgccccg gcccacaacg
      541 ggcacatcgt gaccttcggg accaccagcg agcacccgct cctgtccggc tacgaggaca
      601 tggtccgcag ccagctcgtc gacgccttca tcctcgccga cacccacgag ggtgacccgc
      661 ggccggactg gctggatggc gcgcgcatcc cgtatgccgc cttcggccgc ctctggggtc
      721 gcccggagct gacctcgtgg gccgacgtgg acggtgcgca cggtttgcgc attgccaccg
      781 aacaccttct cgcccaagga tattcgacag tcggcttcct cggctggccg gcggggtcgc
      841 aggtcggcga cgatcgccgc cgcggatggg ccgccgtcca cgaagaccgg agcatgccgc
      901 tgggccccga agccatggcc gtgcaggatc tgggcgccgc caccgcggct gccggaccgt
      961 tgctcgacca ggtcgggcgc ggcggcgcga tcgcctgcgc ctccgacatg ttcgcgctgg
     1021 gtgtcgagcg tgccgctgcc gcccgcgggt ggcgctccgg cacggacatc ggcatctgcg
     1081 gtttcgacgc ctccgtcatc gcggagatcg agtcgctgac cagcgtggcc ctgcccctgt
     1141 acgagatcgc cgatcacctc ctcggcctcc tgcaggaccg gctcggcggc gctcccccgc
     1201 cggcggccgg gctgctcgtc gaacccggct tgacgatccg ctccagttcc acccgatgac
     1261 cgcacgccac cgctcccacc ccgccactcg gcatacccct agaccggtcg accggccggc
     1321 ccgacaagaa agagaaatca tggcacgcac caccgcccgc cgcggcgtct ccctcgccat
     1381 cgccgcgctt ggctccctca ccctcgctgc ctgcggtggc ggcgggttcg aggagaccac
     1441 gtcctccggc gccggcggct ccgcctccgc ttccgggtcg gcctcggcat cggcctccgc
     1501 ttcggcttcg gtgccgaccg gcgctgccct gaagatgctc atcgcctcct ccggcgacgc
     1561 gagacggccg ccgtcaaggc ggcggcggac gtcacctacg acggcaagca gtactgcgtg
     1621 cccaaggact tctcctccct cgccctccag atcaacaccg acatgtggga gaaggccggt
     1681 ctgaccgacg ccgacatccc caccacctgg gaccagttga ccgaggtgtc caagaagctc
     1741 accagcggca aggtcgccgg gctcggcatc ggcgtcggca tcgaccggct cggggccttc
     1801 gtcgtgcaga acggcgggtg gtggctcaac gccgacaaca ccgccggtgc cgccgcggac
     1861 gccaaggtca ccgacgccct gaagtatgtc caggacaacg tcaaggccgg caacttccag
     1921 atgtccaacg acctcggcgc gggctggggt ggcgaggcct tcggcaagca actggccgcg
     1981 atggtcatcg agggcaactg gatcaagggc gccatgaaga acgacttccc cgacgtcaag
     2041 taccagaccg tcgaactgcc cgccggcgcc cagaagggca ccctgatgtt cacccagtgc
     2101 tggggcctgg ccgccgacag ccagaacaag gacgctgcca aggacctcat caagtccctg
     2161 accaccgccg agcagcagat tgcgttcgcc gacgctttcg gcgtcatgcc gtcgcgtacc
     2221 gacgccgccg agacctacaa ggagaagtac cccgaggatg ccgccttcgt cgccggcggt
     2281 gagtacggcc acggcccgat caacctgccg gacatgaccg aggttgtcgc cgatctcaac
     2341 agtcagctcg agaagatgag cacggccgac atcgcgggcg cgaccacggc attcgacgag
     2401 aacgccgcct cggcgatcgg cggctgacac cgactctgcg ccgggccggc cttcccctgc
     2461 gaccggcccg gcgcagcacc cacctggcac cggacggcgg agcatgcgct tcgccggccc
     2521 atgccgacct gcttgattcc gcttgcagcc caaggacatc accatggcgc gt
//
LOCUS       DAOPJG010000290         2570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_46323_length_2570_cov_8.565805, whole genome shotgun sequence.
ACCESSION   DAOPJG010000290 DAOPJG010000000
VERSION     DAOPJG010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46323_length_2570_cov_8.565805"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKU75_16485"
     CDS             complement(<1..421)
                     /locus_tag="PKU75_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPF82526.1"
                     /translation="MSRTVRFTKGHGTRNDFVLLDDRDGEFALTATMAAALADRRSGI
                     GGDGVIRIAPTSSAREDEVRAQADDAAWFMDYRNADGSVAEMCGNGVRVFALYLRREG
                     LAPAEGFGVATRGGVKRVRFGGDDTVIVDMGSWRYEDE"
     gene            complement(444..1361)
                     /gene="miaA"
                     /locus_tag="PKU75_16490"
     CDS             complement(444..1361)
                     /gene="miaA"
                     /locus_tag="PKU75_16490"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPF82527.1"
                     /translation="MPSAHPVVAVVGATATGKSALALDLAERIGGEIVNADAMQLYRG
                     MDIGTAKLPVGERRGIPHHQLDILDVTDEASVAVYQRESRRDLDGISERGGIPLLVGG
                     SGLYVRAALDALDIPPTDPDVRARWEAYAAAHGAEAAHSELARVDPVAAQQILPTNVR
                     RIVRALEVNELTGGPFVAALPRRTYRRATVMVGLHLERDRLDERIGRRTEQMWADGLI
                     DEVERLVAAGLREGRTARAAIGYQQALAQLDGNLREDEAIAETITATRRLVRRQESWF
                     RPDPRIHWMPADDPGLTDAVLRLLDSAAT"
     gene            complement(1376..1954)
                     /gene="def"
                     /locus_tag="PKU75_16495"
     CDS             complement(1376..1954)
                     /gene="def"
                     /locus_tag="PKU75_16495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPF82528.1"
                     /translation="MSVRPITITGEPVLHRRAEVVTDFGPELQTLIDDMLETQAAAHG
                     AGLAAPQVGVGLRVFTWHLANEDGVPEIGVIVNPFVRATRPPDGDPDKHDDAEGCLSV
                     PGLHFPLTRGDRADVTGLDRNGNELAFTATGWFARCMQHEYDHLNGFLYVDRLDEKWK
                     KKARKAIRGEGWGAAGKTWMPGVDPDPFGHDS"
     gene            2098..2286
                     /locus_tag="PKU75_16500"
     CDS             2098..2286
                     /locus_tag="PKU75_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009374413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPF82529.1"
                     /translation="MGLLDDVKGKVDDLVSQNPDKVEELSDQGIAKATEVADSASGGK
                     FGEQIDAAGQKADEAIGE"
BASE COUNT          404 a          892 c          872 g          402 t
ORIGIN      
        1 actcgtcctc ataacgccag ctgcccatgt cgacgatgac ggtgtcatcg ccaccgaagc
       61 gcacccgctt gacgccgccg cgggtggcca cgccgaagcc ctccgccggg gcgagtccct
      121 cgcgccgcag atagagcgcg aagacccgaa ccccgttgcc gcacatctca gccacgctgc
      181 cgtcggcatt gcgatagtcc atgaaccacg cggcgtcgtc cgcttgggcg cgcacctcgt
      241 cctcacgggc gctgctcgtc ggggcgatcc ggatcacccc gtcaccgccg atgccggatc
      301 gccggtccgc gagggccgct gccatggttg cggtcagggc gaactccccg tcgcggtcgt
      361 cgaggagcac gaagtcgttg cgggtgccat gacccttggt gaagcgcaca gtcctcgaca
      421 tggcgaccat tgtcccgtat cgctcaggtg gccgcggaat ccaggagccg cagcaccgcg
      481 tcggtcaggc cggggtcgtc ggccggcatc cagtggatgc gcggatccgg ccggaaccag
      541 ctctcctggc gacgcaccag ccgccgggtc gccgtgatcg tctcggcgat cgcctcatcc
      601 tcgcgaaggt tcccgtccag ttgcgccagc gcttgttgat agccgatggc cgcgcgcgcg
      661 gtcctgccct cgcgcaggcc ggccgcaacc agccgctcga cctcgtcgat cagtccgtcg
      721 gcccacatct gctcggtccg ccggccgatc cgctcgtcga ggcggtcgcg ctccaggtgt
      781 aggccgacca tgaccgttgc ccggcggtag gttcgccgcg gcagggccgc gacgaatggg
      841 ccgccggtca gctcgttgac ctcgagggcc cggacgatac gccgcacatt ggtcggcagg
      901 atctgctgcg ccgcaacggg atccactctg gccagttcgg aatgcgcggc ctcggcaccg
      961 tgcgccgcgg catacgcctc ccaccgcgcg cgcacgtccg gatccgtggg cgggatgtcg
     1021 agggcgtcga gggcggcgcg gacatacaac ccgctcccac cgacaagcaa gggtatgccg
     1081 ccccgctcgc tgatcccgtc gaggtcgcgt cggctttccc gttggtagac agcgacgctc
     1141 gcctcgtcgg tcacgtcgag gatgtcgagt tggtggtgcg gtatgccgcg ccgctccccc
     1201 accgggagct tggccgtgcc gatgtccatc ccgcgataga gctgcatcgc gtcggcgttg
     1261 acgatctccc cgccgatgcg ctcggcgaga tcgagcgcca gcgcggattt cccggtcgcc
     1321 gtcgccccga ccacggccac gacggggtgc gcggacggca taccgcccgg cgtcatcagc
     1381 tgtcgtgccc gaagggatcg ggatcgacac caggcatcca cgtcttgccg gcggcccccc
     1441 agccctcacc gcgaatcgcc ttgcgcgcct tcttcttcca cttctcgtcg agccggtcga
     1501 catagaggaa accgttgagg tggtcgtatt cgtgctgcat gcaccgggcg aaccagccgg
     1561 ttgccgtgaa ggccagctcg ttgccgttgc ggtcgagacc cgtgacgtcc gcgcggtccc
     1621 cgcgtgtgag ggggaagtgc aggcccggca cggacaggca gccttcggcg tcgtcgtgct
     1681 tgtccggatc gccgtcgggc ggacgggtcg cgcgcacgaa gggattgacg atcacgccga
     1741 tctcgggcac gccgtcctcg ttggccaagt gccacgtgaa gacgcgcagc ccgactccca
     1801 cctgcggggc agcgagcccg gcgccatggg cggcggcctg agtctcgagc atgtcgtcga
     1861 tgagagtctg cagctcaggg ccgaagtcgg tgacgacctc ggcccggcgg tgcagcaccg
     1921 gctctccggt gatggtgatg gggcgcacgg acatggtcac cattgtccgg cacgcgtgcc
     1981 gtgcgaagtc acagaagttt ctccggcccc aggcatggcc aacggtcatg acacgtgtct
     2041 agagtgttcc tacgcgatcg ccggccggca tcgtgagtcg ggcggaagga gtcacgcatg
     2101 ggtttgctgg acgacgtcaa gggcaaggtc gacgacctcg tgagccagaa ccccgacaag
     2161 gtcgaagagc tcagcgacca gggcatcgcg aaggccaccg aagtggccga cagcgcatct
     2221 ggtggcaaat tcggcgagca gatcgacgca gccggccaga aggccgacga ggccatcggc
     2281 gagtagtcgc cccacacgac gccgcgagcg ggccctggtg caccagggcc cgctctctta
     2341 tgcccggaac tgggcacttt gtccggccac ccgaaagggt gagaaacggc ctcctcttaa
     2401 ggtcacgcca aggcgttctg agcgtgtaga catggcgcta ccggggtcgg caccgaggcg
     2461 gaccccatcg aaccaccaag gagttgtgtc gtgggtttcc tcgatgacat gaaggacaag
     2521 gcgagcgaca tgctcgactc cgcgaaggac gccgtgagcg gcgctgccga
//
LOCUS       DAOPJG010000291         2553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_46751_length_2553_cov_7.841873, whole genome shotgun sequence.
ACCESSION   DAOPJG010000291 DAOPJG010000000
VERSION     DAOPJG010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46751_length_2553_cov_7.841873"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKU75_16505"
     CDS             complement(<1..254)
                     /locus_tag="PKU75_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPF82530.1"
                     /translation="MRLTALLDFGSTATLTEGEIPCQSNDPELWFADTPEGVEFAKAL
                     CVSCPMIEACLDGALSRREAAGVWGGQYFVDGVVVARKRPR"
     gene            complement(484..684)
                     /locus_tag="PKU75_16510"
     CDS             complement(484..684)
                     /locus_tag="PKU75_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPF82531.1"
                     /translation="MSNSMIPTWTPLSMTAACAQVGVVVRGRGHVCVSSVDLARSLDQ
                     TAYATKTTFATKTTGSPPGVAP"
     gene            complement(797..>2553)
                     /locus_tag="PKU75_16515"
     CDS             complement(797..>2553)
                     /locus_tag="PKU75_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HPF82532.1"
                     /translation="KAAAVAEAAGRLRLRLGRDELRDAAAEIEWAKVGMHTADSYAAA
                     ATRARRTAPGMDLTAMARLLAAYEEVKGERGVIDFEDVLLVTLGMLRSEPRMAAAVRD
                     QYRHFVVDEYQDVNALQQTLLLAWLGERHEVCVVGDPAQTIYSFTGASADHLRDFTRV
                     HPGAASVELVRNYRSTPQVVTLANGLARGPSGSRRWGGVELLAQRDAGPGPVLTAYDD
                     DAGEAAGVATVIERAIAAGTPAREIAILFRTNGQSEAFETALAEKAIPYLIRGGERFF
                     QRREVREATVLLRGGVRSDDSSVELPELVRSILTGAGWGPVAPVSGGAARERWESLEA
                     LAVLADQFVAGMPAARLADFVRELDERAAAQHAPSVEGVTLASLHAAKGLEWDLVVLA
                     GVSDGLMPIVMAETNAEIDEERRLLYVGVTRARRELHLTWARAKVAGGRGNRRPSRFL
                     GGLLEGAPGADGPPARKRTKERKLATCRTCGTVLESAAERTTGRCATCPATYDEATFE
                     ALRTWRSLVAKAAKWPAYVIFTDATLTAIAEASPEDEASLARISGVGQRKLALYGDAV
                     LAVLGGGDPAQAAAEIKK"
BASE COUNT          365 a          911 c          888 g          389 t
ORIGIN      
        1 cggggccgct tgcgggcgac gacgacacca tcgacgaagt actgaccacc ccagacgccg
       61 gcggcttcgc gccgggagag ggcgccgtcc aggcacgcct cgatcatcgg gcagctgacg
      121 cagagcgcct tggcgaactc gacgccctcc ggggtgtccg cgaaccacag ttcggggtcg
      181 ttggactggc acgggatctc cccctcggtg agagtggccg tggagccgaa gtcgagcagc
      241 gctgtgagcc gcatggggtg ggtcacctcc ttggtcgtcc gccgggtatg gcggtggtgt
      301 ggtgcgccgg gtccggcgcg gtcctgcgtg tgacaggtct ggtcgtgcag ggcggtggtt
      361 ggcatctgcg tcccggattc agttgtgtcg gaaggcccgt cgtctgggaa tgcccaggaa
      421 tgacgaaggc cgcggatcct gtatcggatc cgcggcctgg aggtcagggc ctgtgacgtg
      481 ttgtcacggg gcgactccag gcggagatcc ggtggtcttg gtggcgaagg tggtcttggt
      541 ggcgtatgcg gtctggtcga gggagcgggc gagatcgaca gagctcacgc agacgtgacc
      601 gcgaccccgg acgacgacac cgacctgcgc gcaggcagcc gtcatcgaca gcggggtcca
      661 ggtggggatc atgctgttgc tcattacggt tgcctccttc gatcaggtcg tgagccaagg
      721 gtgagcgccg ggtggcgtcc tcagtgaggg tagacgcacc taggggtggc tgtcatccga
      781 attatcgagt cgttgctcat ttcttgatct cggcagccgc ctgagccggg tcgccgcccc
      841 cgaggactgc cagcaccgca tcgccgtaga gtgccaattt gcgttgtccc acaccggaaa
      901 tccgcgccaa cgacgcttcg tcctcgggcg acgcctcggc gattgccgtc aatgtcgcgt
      961 ccgtgaaaat gacgtacgcc ggccacttgg ctgccttggc caccagactc cgccacgtcc
     1021 ggagcgcctc gaacgtcgcc tcgtcatagg tcgccgggca ggtcgcgcag cgcccggtgg
     1081 tccgttcggc cgcggactcc aggacggtcc cgcacgtgcg acaggtcgcg agcttgcgct
     1141 ctttggtgcg cttgcgggcc ggcggcccgt ccgctcccgg cgccccctcg agcaggccgc
     1201 cgaggaaccg cgagggccga cgattgcccc gccccccggc gaccttggct cgcgcccagg
     1261 tcaggtgcaa ctcgcgccgg gcccgggtca caccgacata cagcagccgg cgctcctcgt
     1321 cgatctcggc attggtctcg gccatgacga tcggcatcag cccgtccgaa acccccgcca
     1381 ggacaacgag atcccattcc aaccctttgg cggcgtgcaa cgacgcgagc gtgaccccct
     1441 cgacggacgg cgcgtgctgc gccgcggccc gctcgtcgag ctcgcgcacg aagtcggcga
     1501 ggcgagcggc cggcataccc gcgacgaact ggtcggcgag caccgcgagc gcctcgagcg
     1561 actcccagcg ctcccgcgcc gcacccccgc tgaccggcgc caccggcccc cagccggcgc
     1621 cggtgaggat cgagcgcacg agctcgggca actcgacgga ggagtcgtcg ctgcgcaccc
     1681 caccccgcag gagcaccgtg gcctcacgca cctcacgccg ctggaagaag cgctcaccgc
     1741 cgcggatcag atagggaatc gccttctccg ccaacgcggt ttcgaacgct tcgctctgcc
     1801 cgttggtgcg gaagaggatg gctatctccc gcgcgggcgt gcctgcggcg atggcgcgct
     1861 cgatcaccgt cgcgacgccc gcggcctcgc ccgcgtcatc gtcgtatgcc gtgagcaccg
     1921 gccccggtcc cgcgtcgcgt tgcgcgagca actcgacccc gccccaccgc cgggacccgc
     1981 tcggcccgcg cgccagccca ttggcgagcg tgacgacctg gggggtggag cggtagttgc
     2041 ggaccagctc gacgctcgcc gcacccgggt ggactcgggt gaagtcgcgc aggtgatcgg
     2101 cgctggcgcc ggtgaaggag tagatggtct gggcgggatc gccgacgacg cacacctcgt
     2161 gccgctcccc cagccaggcg aggagcagcg tctgctggag ggcgttgacg tcctggtatt
     2221 cgtcgacgac gaagtggcga tactggtcgc gcacggcagc agccatccgc ggctcgctcc
     2281 gcagcatccc caaggtgacg agcaggacgt cctcgaagtc gatgaccccc cgctcgccct
     2341 tgacctcctc gtaggccgcc agcagccgcg ccatcgccgt caggtccatg cccggggccg
     2401 tgcggcgggc acgcgtggcg gccgcagcat acgaatcggc ggtgtgcatg cccaccttcg
     2461 cccactcgat ctcggccgcc gcgtcgcgca gctcgtccct gcccaggcgc agccgcagtc
     2521 gcccggcggc ctcggccacc gccgccgcct tgc
//
LOCUS       DAOPJG010000292         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_54934_length_2281_cov_6.736748, whole genome shotgun sequence.
ACCESSION   DAOPJG010000292 DAOPJG010000000
VERSION     DAOPJG010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54934_length_2281_cov_6.736748"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PKU75_16520"
     CDS             <1..791
                     /locus_tag="PKU75_16520"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HPF82533.1"
                     /translation="APGDVAAGFTQTVAMIGLADERPKPYEPSPMDPAASVLDAFVGE
                     LQRAIPDATVTADDIPDLRATPAPTEATVVARGQSAAYRDVLAEALDTSDNALTENLA
                     RQAALQAGRDGSFADNAAFVVDTLKAKGYDLTGTTLLDTSGLTRGQTSTAALLSQVTA
                     EALAGKLPGLQDVIARLPVAGLDGTLFDRFRTEPANAAAGVARAKTGTLTGISSLVGT
                     TVDADERELTFVIVADQVPASSGTLAARAALDRFVATLTACGCR"
     gene            849..1928
                     /locus_tag="PKU75_16525"
     CDS             849..1928
                     /locus_tag="PKU75_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPF82534.1"
                     /translation="MPDAQVVPTDEAPPRFVDWDFAKATGRRLVGAGPEVSAAEAGDI
                     VAKIRAAAAAAQEPVAETSRMHAPGIAPPAQVIDRPTWIDLNLDSMAALMTPVFVKVA
                     RDRTVSANAPGMGVGAKITGAESGALMAFMASKVLGQYDLAPQGTPRLLLVAPNLVAV
                     QRELDVDPDDFRAWVCMHEETHRIQFTAVPWLREHMIERATTLAGELTPTPQELGQRM
                     QEMVKRLPEALGSGGGGLADLFATPEQRAKIAEVTAIMSLLEGHADVVMDDVGPKVIP
                     TVADIRAKFTERRKGAGAADRMLRRLLGLEAKMAQYKDGAGFVRGVMDKVGVDGFNAV
                     WTSPETLPLPAEIHDPQAWVRRVHG"
BASE COUNT          357 a          848 c          760 g          316 t
ORIGIN      
        1 gggctcccgg tgacgtcgcc gccggtttca cccagacggt cgcgatgatc ggcctcgccg
       61 acgagcgccc caagccctac gaaccctccc cgatggaccc tgcggcgtcg gtgctggacg
      121 ctttcgtcgg ggagctgcag cgcgccatac ccgacgccac ggtcacggcc gacgacatcc
      181 cagacctgcg tgccaccccg gcccccacgg aggcgacggt cgtcgcgcgg gggcagtccg
      241 cggcataccg tgacgtgctc gccgaggccc tcgacaccag cgacaacgcc ctcaccgaga
      301 acctcgcgcg ccaggcggca ctccaggccg gccgcgacgg gtcgttcgcc gacaacgccg
      361 cattcgtcgt cgacacgttg aaagccaagg gctatgacct gacgggcacc actttgctcg
      421 acacctccgg cctgaccagg gggcagacga gcacggccgc gttgctctcg caggtgaccg
      481 cggaagcgct cgcgggcaaa ctgcccggcc tccaggacgt catcgcgcgg ctgccggtgg
      541 ccggcctgga cgggaccctg ttcgaccggt tccgcaccga gcccgccaac gccgcggccg
      601 gtgtcgcccg cgccaagacc ggcaccctga cgggcatttc gtcacttgtg ggcactacgg
      661 tcgatgcgga cgagcgcgaa ctgaccttcg tcatcgtggc cgaccaggtg cccgcctcga
      721 gcggaacatt ggcggcccgg gccgcgttgg accggttcgt cgcgaccctc acggcctgtg
      781 gatgtcgata gacgatggac gaaaggcgcc cgcgtatgag tgagcaccct caagacacca
      841 tccccgcgat gccggacgcg caggtcgtgc cgaccgacga ggcgccaccg cgattcgtcg
      901 actgggactt cgccaaggcc accgggcgcc gcctcgtcgg cgccggaccg gaggtcagtg
      961 ccgccgaggc cggcgacatc gtcgccaaga tccgggcagc tgccgcggcc gcccaggagc
     1021 ccgtcgccga gacctcgcgg atgcacgccc ccggcatcgc cccgcccgcg caggtgatcg
     1081 accgccccac gtggatcgac ctcaacctcg actcgatggc cgcgctgatg acgccggtct
     1141 tcgtcaaggt cgcgcgcgac cgcacggtgt cggccaacgc acctgggatg ggtgtcggag
     1201 ccaagatcac cggcgccgag tcgggtgcgc tcatggcgtt catggccagc aaggtgctcg
     1261 gccagtacga cctggccccg caaggcacgc cgcgcctgct cctcgtggcc cccaatctcg
     1321 ttgccgtgca acgcgaattg gatgtcgacc ccgacgactt ccgcgcgtgg gtgtgcatgc
     1381 acgaggagac ccaccgcatc cagttcaccg cggttccgtg gctgcgcgag cacatgatcg
     1441 agcgggcgac gaccttggcc ggcgagctga cccccactcc gcaggagctc gggcagcgta
     1501 tgcaggagat ggtcaagcgt ctccccgagg ccctcggttc gggcggcggc ggcctggccg
     1561 atctgttcgc cacgcccgag cagcgggcca agatcgccga ggtcacggcg atcatgagcc
     1621 tgctcgaggg gcacgccgat gtcgtgatgg acgatgtggg ccccaaggtc atcccgacgg
     1681 tggcggacat ccgcgccaag ttcaccgagc gccgcaaggg tgccggcgcc gccgaccgga
     1741 tgctgcgccg cctcctcggc ctggaggcca agatggcgca gtacaaggac ggcgcgggct
     1801 tcgtccgcgg ggtcatggac aaggtcggcg tcgacggctt caacgcggtc tggacctcac
     1861 ccgagaccct cccgctgccg gccgagatcc acgacccgca ggcctgggtc cgccgcgtcc
     1921 acggctgatc gaggacggga cgcgcgactc caagatctcc atctgtcggg tgctgtccgg
     1981 cgggtcaacc actcggttac ggaaggcagc cccattgagc aacactcggc ggcgcgcgtg
     2041 cttccagctc aggtctgagt gcctctcgga tacgaggtgg tgcgcctgat ctggcgcgaa
     2101 ctcgacgacc ccgtcggcgt catcgctcgg atccgcgacg ccttcgcccg cgccttgacc
     2161 cggcccgctc caccgcctcg acccgccccg gaaccccgaa ccgcctcgga aggcgcgaac
     2221 agcctggttt agcccgaact ccctggcaca tatctgccgg agtccgggct aagccaggca
     2281 g
//
LOCUS       DAOPJG010000293         2174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_58792_length_2174_cov_7.840491, whole genome shotgun sequence.
ACCESSION   DAOPJG010000293 DAOPJG010000000
VERSION     DAOPJG010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58792_length_2174_cov_7.840491"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKU75_16530"
     CDS             complement(<1..369)
                     /locus_tag="PKU75_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HPF82535.1"
                     /translation="MTVRVGINGFGRIGRNFFRAVQASGADIEIVAVNDLTDNKTLAT
                     LLKYDSVLGRLDGDVTFDEESITVNGKSFKVFEDRDPANLDWKSVGADVVIESTGFFT
                     DATKAKAHIDGGAKKVIISAP"
     gene            complement(524..1504)
                     /gene="whiA"
                     /locus_tag="PKU75_16535"
     CDS             complement(524..1504)
                     /gene="whiA"
                     /locus_tag="PKU75_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPF82536.1"
                     /translation="MAMTASVKDELSRLEVTKTCCRKSEVASMLRFAGGLHIIGGKIV
                     VEAELDTASVARRLRKEVYELYGHRSEVVVMAAGGLRKGSRYVVRIVEGGELLARQTG
                     LIDARGRPVRGLPAKVVGGSMCDSVAAWRGAFLAHGSLTEPGRSSALEITCPGPEAGL
                     ALVGAARRLEIPAKARDVRGVDRVVIRDGDAIGAMLTRMGAHDAVIAWEDRRTRREVR
                     ATANRLANFDDANLRRSAQAAVAAGARVQRALEILGDDVPDHLRVAGDLRMAHKQASL
                     EELGALAQPPMTKDAVAGRIRRLLAMADKRAGELGVPDTEAVISADLLDD"
     gene            complement(1584..>2174)
                     /locus_tag="PKU75_16540"
     CDS             complement(1584..>2174)
                     /locus_tag="PKU75_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HPF82537.1"
                     /translation="RLLNARGRVLPMAAVPLDIAAQVALDPDRLDLCTEIRGQDGVAK
                     TSGHVRSVALIPPDPPAHPEAISALAQADWIIFGPGSWFTSVLPHLLVPKLRQALVAS
                     PARRLLTLNLEMNTLETRGFSAVRHIETLREHAPDLRLDIVLADPTIVDDVAALEEAV
                     AGLGARLVIADVSNPRTPGAHDPLRLAAAYRDIFGD"
BASE COUNT          316 a          749 c          777 g          332 t
ORIGIN      
        1 cggggcggag atgatgacct tcttggcgcc gccgtcgatg tgggccttgg ccttggtggc
       61 gtcggtgaag aaaccggtgg actcgatgac gacatcggcg cccacggact tccagtccag
      121 gttggccggg tcgcggtcct cgaagacctt gaaggacttg ccgttgacgg tgatcgactc
      181 ctcgtcgaac gtgacgtcgc cgtcgaggcg gcccaggacc gagtcgtact tcaggagggt
      241 cgcgagcgtc ttgttgtccg tcaggtcgtt gacggccacg atctcgatgt cggcgccgga
      301 cgcctggacg gcgcggaaga agttgcggcc gatccggccg aagccgttga tgccaacgcg
      361 aacagtcatt gctgaaagcc ttgccttccg tagggggtat gcggtgtcgc ctctatgcgg
      421 cacctgaccg atacccagcc tattgcgccc cggttcgcca aggcaccccg cccagccatc
      481 ggacgctttt ctcggctgcg tctcagagcg tgagaggcgc gactcagtcg tcgagcaggt
      541 cggcggagat caccgcctcg gtgtccggaa cgccgagttc acccgcccgt ttgtcggcca
      601 tcgccagcag ccgccgaata cgcccggcga cggcgtcctt ggtcatgggt ggctgcgcca
      661 gcgccccgag ctcctccaga gaagcctgct tgtgcgccat ccgcaggtct ccggcgacgc
      721 gcaggtgatc gggcacgtcg tcgccgagga tctccagcgc ccgctgcacg cgggcgccgg
      781 cggcgacggc cgcctgggcc gagcggcgca ggttggcgtc gtcgaagttc gcgagccggt
      841 tggcggtggc gcgcacctcc cggcgggtgc ggcggtcctc ccaggcaatg acggcgtcat
      901 gcgcgcccat ccgggtcagc atcgccccga tggcgtcgcc gtcccggatg acgacccggt
      961 cgacgccgcg cacatcgcgg gccttggccg ggatctccag ccgccgcgcg gcaccgacga
     1021 gcgccaggcc ggcttccggg ccgggacagg tgatctcgag cgccgacgac cggccaggct
     1081 cggtcagcga gccatgggcg aggaaggcac cacgccaggc ggcgaccgag tcgcacatcg
     1141 agccgccgac caccttggcg ggcagtccgc gcaccgggcg gccgcgcgcg tcgatcagtc
     1201 cggtctgccg ggccaggagt tcaccgccct cgacgatccg cacgacatac cggctgccct
     1261 tgcgcaaccc gccggcggcc atgacgacga cctcgctgcg gtgcccatag agctcgtaga
     1321 cctctttgcg cagccgccgg gccacgctcg cggtgtcgag ttcggcctcg acgacgatct
     1381 tgccgccgat gatgtgcagg ccaccggcga accgcagcat cgaggcaacc tcggacttgc
     1441 gacagcaggt cttggtcacc tcgaggcgac tgagttcgtc cttgacggaa gcggtcatgg
     1501 ccatcgggct atggtgccat ccgccggcgg gtctcgctga acccgggggc cgcgctcttg
     1561 gtcacctcat cccgtccgcc gcttcagtcg ccgaagatgt cccggtatgc cgcggcgagg
     1621 cgcagcgggt cgtgggcgcc gggcgttctg gggttggaaa cgtccgcgat gacgagcctc
     1681 gcgccgaggc cggccacggc ctcctcgagg gcggccacgt cgtcgacgat cgtggggtcg
     1741 gcgaggacga tgtcgagccg caggtcgggg gcgtgctcgc gcagcgtctc gatgtgccgg
     1801 accgcggaga acccccgcgt ctcgagggtg ttcatctcga gattgagggt gagcaggcgg
     1861 cgcgccgggc tcgcgacgag ggcctgccgc agcttcggca ccagcaggtg cggcagcacc
     1921 gaggtgaacc acgaccccgg gccgaagatg atccaatccg cttgtgccag agcggaaatc
     1981 gcctcggggt gggcgggcgg atcgggcggg atcagcgcca cggaccgcac gtgaccactg
     2041 gttttcgcca ccccgtcctg gccgcgaatc tcggtgcaca gatcgagccg gtccggatcc
     2101 agggccactt gcgcggcgat gtcgagcggg accgccgcca tcggcagcac ccggccgcgg
     2161 gcattgagca gccg
//
LOCUS       DAOPJG010000294         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_63313_length_2062_cov_7.810663, whole genome shotgun sequence.
ACCESSION   DAOPJG010000294 DAOPJG010000000
VERSION     DAOPJG010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63313_length_2062_cov_7.810663"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1182
                     /gene="nudC"
                     /locus_tag="PKU75_16545"
     CDS             175..1182
                     /gene="nudC"
                     /locus_tag="PKU75_16545"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HPF82538.1"
                     /translation="MADDSYSDDAKPLGEIPLSAAGLDRRAERRGQAGLVAGLLADPA
                     TLVLPVAAGRAPGRQAVVREQDAGAGSRLVDADGVTHDVLPTPAGALSAAAPRLAWRA
                     PEAGDTAYDAVYLGEADGCSRLALLLPPDQAGDGWFTLRGEGADLEPVDAEALTTGVA
                     LANWHATHTHCPRCGAETEIANAGWTRRCPADGSEHFPRTDPAVIMAVIDADDRLLLA
                     RHPNWPEGRLSVLAGFVEPGESLEMAVAREVFEEVGVVVRDVRYLGDQPWPFPNSLML
                     GFEARADDPTLVLNETEIAEAFWVTREEYHAKVDEGAWGGGTSLSISRRLILRWLHRG
                     G"
     gene            complement(1281..1538)
                     /locus_tag="PKU75_16550"
     CDS             complement(1281..1538)
                     /locus_tag="PKU75_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097573 - glutathione oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HPF82539.1"
                     /translation="MPVETPAAGSVTMFSTTWCGYCTRLKSQMDREGIAYTEVNIEHD
                     PDAEAFVMSANNGNQTVPTLLFPDGSTATNPSLIEVKKKLS"
     gene            1626..>2062
                     /locus_tag="PKU75_16555"
     CDS             1626..>2062
                     /locus_tag="PKU75_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPF82540.1"
                     /translation="MTFPDADAILAALDPEQREVARTPVGPMRVIAGAGTGKTRAITH
                     RIAFGVRSGAYAAPQVLALTFTARAAGEMRTRLRELGVAGVQARTFHAAALRQLHYFY
                     PQVIGGAAPEVMASKAAAVAEAAGRLRLRLGKNELRDAAAEIE"
BASE COUNT          287 a          681 c          774 g          320 t
ORIGIN      
        1 catggtccag gccggctacc tcggacgcaa gacgaagaag ggcttccgca cctacgcctg
       61 aggtcctcgc tcacgagctt ttcgagcgtc aggtgagtgg cagcgacgtc ctcatgtggc
      121 tgactctcac gggacggcag tcgccggtcg ccgggcatgg gcgaagatga ctccgtggct
      181 gatgactcct attccgatga tgccaagccc ctgggcgaga tccccctgag cgcggcgggc
      241 ttggaccggc gggcggaacg acgggggcag gccggcctcg tcgccggatt gctcgccgat
      301 ccggcgacgc tcgtgctgcc ggtggcagcg gggcgggcac cggggcggca ggcggtcgtc
      361 cgagagcagg acgccggggc cgggtcgcgg ctggtcgacg ccgacggcgt cactcacgat
      421 gtcctgccca cgcccgccgg cgctctttca gcagcggcac cccggctggc gtggcgggcg
      481 ccggaggcgg gggacacggc atacgacgcg gtgtatctcg gcgaagccga cgggtgttcc
      541 cggctcgcac tgctgttgcc gccggatcag gccggagacg gctggttcac cctgcgtggc
      601 gaaggggccg acctcgagcc cgtcgacgcc gaggctctca ccaccggcgt cgccctggcc
      661 aattggcatg ccacacatac ccattgcccg cggtgcggcg ccgagaccga gatcgccaac
      721 gccggctgga cccggcgctg cccggccgac gggagcgaac acttcccccg caccgacccg
      781 gcggtcatca tggcggtgat cgacgccgac gaccggctgt tgctggcgcg tcacccgaac
      841 tggcccgagg gccggctctc ggtgctggcc gggttcgtcg agccgggcga atcgctggag
      901 atggctgtgg ctcgcgaggt gttcgaggag gtcggggtcg tcgtgcgtga tgtgcgctat
      961 ctcggcgatc agccctggcc cttccccaac tcgctcatgc tcggcttcga ggcgcgagcc
     1021 gacgacccca cgctggtgct gaacgagacc gagatcgccg aggccttctg ggtgacgcgc
     1081 gaggagtacc acgccaaggt cgacgagggc gcctggggtg gcggcacctc cttgagcatc
     1141 tcgcgccggc tcatcctgcg gtggctgcat cggggcggct gaccgcgccc tggaaggcct
     1201 gggccgatca cgtcagggac gaggcttttc caggcccagg gccgatcatc cggcggacca
     1261 ccggatgatc gaggacgtct tcaggacagc ttcttcttca cctcgatgag ggaggggttg
     1321 gtggccgtgc tgccatcggg gaagagcagg gtggggacgg tctggttgcc gttgttggcc
     1381 gacatgacga aggcctccgc gtcggggtcg tgctcgatgt tgacctcggt gtaggcgatg
     1441 ccctcgcggt ccatctggga cttcaggcgt gtgcagtagc cgcaccaggt cgtcgaaaac
     1501 atggtgacgc ttccggcggc cggggtctcg acgggcatac ggggctcctt gaggtgtggg
     1561 acttacggtg tggataacgc gggactgatt gggacaacgt cggtcggggg tcgtaccttc
     1621 ccatcatgac ctttcccgac gccgacgcga tcctcgcggc gctcgatccc gagcaacgcg
     1681 aggtggcgcg cacgccggtc gggccgatgc gggtgatcgc cggcgcgggc acgggcaaga
     1741 cccgcgcgat aacccaccgc atcgcgttcg gggtccgctc cggggcgtat gccgcgccgc
     1801 aggtcctcgc gctcaccttc accgcgcggg cggccgggga gatgcgcacg cgcctgcgcg
     1861 agctgggcgt ggccggggtg caggcgcgca ccttccacgc cgcggccctg cgtcagctgc
     1921 actacttcta tccgcaggtc atcgggggtg ccgcgcccga ggtgatggcg agcaaggcgg
     1981 cggcggtggc cgaggccgcc gggcgactgc ggctgcgcct gggcaaaaac gagctgcgcg
     2041 acgcggcggc cgagatcgag tg
//
LOCUS       DAOPJG010000295         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_74493_length_1844_cov_6.150922, whole genome shotgun sequence.
ACCESSION   DAOPJG010000295 DAOPJG010000000
VERSION     DAOPJG010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74493_length_1844_cov_6.150922"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..355
                     /locus_tag="PKU75_16560"
     CDS             5..355
                     /locus_tag="PKU75_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPF82541.1"
                     /translation="MPARPGVLATTRKDGRPHAAPIWYDLDDDGTIVFNTGADTLEGR
                     TLKRTRYASLTVQDDRPPFSFVTVAGRVDLIDDLNCARRVSATRRVSAGVLGVSRSLD
                     DRGADSTAVLKGVC"
     gene            complement(381..1346)
                     /locus_tag="PKU75_16565"
     CDS             complement(381..1346)
                     /locus_tag="PKU75_16565"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HPF82542.1"
                     /translation="MNATIAGFEPFVGEHCETTATGNLLHAAGLPLSEPMMFGLGQGL
                     SYGIFTFKGAPAPFIGGRTRPEQVTQDLASRLGLDVDVRTTRSPRRAWANVADFVDQG
                     IPVGVKLNMRLLDYDTTGADFAGHFVAAYGYDGERVYVVDTRGQGGARVTSRASFEAA
                     RLWKGPMSSNALTWTIAAPHPIALRSALPGAIAATARAFLSPPIANFGAKGIRKTARL
                     IRTWPQEYAAADLAGIGHLMEHGGTGGGLFRRMYAAFLREAGGVLGDASLLDVAEQFE
                     IAAGLWSQVAAALEQVAGPGSLGRAAGLLHEIADVEETAMQALAD"
     gene            complement(1343..>1844)
                     /locus_tag="PKU75_16570"
     CDS             complement(1343..>1844)
                     /locus_tag="PKU75_16570"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPF82543.1"
                     /translation="TGYTITQLVDERGLGAWAGVASSSVYNGLSRVEHRALAQSAPDD
                     TKTGRGPRGRCYTLNPAGKRALRAALREALSTTRENDPRFNLALSGIDTLPATTAASC
                     LRERAAYLAAEHTRLSAIRDAQAHEPTSAAGLLFDRILHAVEAERAWSAAAAQQIASR
                     TERTDR"
BASE COUNT          249 a          617 c          708 g          270 t
ORIGIN      
        1 cgctctgccc gcgcggccgg gtgtgctggc gacgacgcgc aaggacggac ggccccacgc
       61 ggcgccgatc tggtacgacc tcgacgacga cggcacgatc gtcttcaaca ccggtgccga
      121 caccctcgag ggccgcacgc tcaaacggac ccggtatgcc agtctcaccg tccaagacga
      181 ccgcccaccc ttctcgttcg tcaccgtcgc cgggcgggtg gatctgatcg atgacctgaa
      241 ctgcgcgcgt cgagtgagcg cgacacgccg agtttcggcg ggcgttctgg gcgtgtcgcg
      301 ctcactcgac gaccgggggg ccgactcgac ggcggttctc aagggggtgt gctgagcacg
      361 cgggtgatgc aggcggcggg tcagtcggcg agggcctgca tggcggtctc ctcgacgtcg
      421 gcgatctcgt ggaggagccc ggcggcgcgg ccgagactgc ccggcccggc cacctgttcg
      481 agcgcggccg cgacctgcga ccacaggccg gcggcgatct cgaactgctc cgccacgtcg
      541 aggagggacg cgtcgccgag gacgccgccc gcctcgcgca ggaaggcggc atacatccgg
      601 cggaagagcc cgccgccggt gccgccgtgc tccatcaggt gaccgatgcc ggcgaggtcg
      661 gcggcggcat actcctgcgg ccaggtgcgg atgaggcgcg cggtcttgcg aatgcccttg
      721 gcgccgaagt tggcgatcgg gggactgagg aaggcccgag ccgtggcggc gatcgcaccg
      781 ggcagggctg atcggagcgc gatcgggtgg ggcgcggcga tggtccaggt cagggcgttg
      841 gacgacatgg gtcctttcca cagccgcgcg gcctcaaagc tcgcccggct cgtcacccgg
      901 gcaccgccct ggccgcgggt atccacgaca tacacgcgct cgccgtcgta gccgtaggcc
      961 gcgacgaagt ggccggcgaa gtccgccccg gtggtgtcgt agtcgagcag ccgcatgttc
     1021 agcttcaccc cgacggggat gccctggtcg acgaagtcgg ccacattggc ccacgcccgc
     1081 cgaggcgagc gcgttgtgcg gacgtcgacg tcgagtccca gccggctggc gaggtcctgg
     1141 gtgacttgct cggggcgggt gcggccgccg atgaagggcg cgggtgcgcc cttgaaggtg
     1201 aagatgccgt agctgagccc ctggccgagc ccgaacatca tcggctcgct gagtgggagg
     1261 ccggcggcgt gcaggagatt gccggtcgcc gtcgtctcgc agtgttcgcc gacgaagggc
     1321 tcgaagccgg cgatggtggc gttcatcggt cagtcctctc tgttcgggac gcgatctgct
     1381 gggcggcggc cgcgctccac gcccgctcgg cctcgacggc gtgcaggatc cgatcgaaaa
     1441 gcaggccggc agcggaggtg ggttcgtggg cctgggcgtc gcggatggcg ctcaggcggg
     1501 tgtgctccgc cgcgaggtat gccgcccgct cccgcaaaca ggaagccgcg gtcgtcgctg
     1561 ggagagtgtc gatgccggag agggcaaggt tgaaccgcgg gtcgttctcg cgcgtcgtgc
     1621 tcagcgcctc gcgcagcgcc gcccgcagtg cgcgcttgcc ggcggggttg agggtgtagc
     1681 accgcccgcg cgggccgcgt ccggtcttgg tgtcgtcggg cgccgactgc gcgagggccc
     1741 ggtgctccac ccgactcagc ccgttgtaga ccgatgagga ggcgacgccg gcccaggcgc
     1801 ccaggccccg ttcgtcgacc agctgggtga tggtgtagcc ggtg
//
LOCUS       DAOPJG010000296         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Nostocoides australiense isolate AMR_MDS_3283
            NODE_82333_length_1723_cov_7.351918, whole genome shotgun sequence.
ACCESSION   DAOPJG010000296 DAOPJG010000000
VERSION     DAOPJG010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865018
            Sequence Read Archive: SRR5007151
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nostocoides australiense (wastewater metagenome)
  ORGANISM  Nostocoides australiense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Nostocoides.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:02:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,289
            Genes (coding)                    :: 3,260
            CDSs (with protein)               :: 3,260
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 9 of 29
            Pseudo Genes (incomplete)         :: 17 of 29
            Pseudo Genes (internal stop)      :: 4 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Nostocoides australiense"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82333_length_1723_cov_7.351918"
                     /isolate="AMR_MDS_3283"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:99480"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1723)
                     /locus_tag="PKU75_16575"
     CDS             complement(<1..>1723)
                     /locus_tag="PKU75_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HPF82544.1"
                     /translation="LIAHNGEINTVKGNRNWMAARESQLVSDLIPGDLSRLSPICPPG
                     GSDSASFDEVLELLHLGGRSLPHAVLMMIPEAWENHEEMDADRRAFYEFHSMTMEPWD
                     GPACVTFTDGTQIGAVLDRNGLRPGRFWVTDDGLVVLASESGVLDIAPEKVIRKGRLQ
                     PGRMFLVDTAQGRIIEDEEIKSKLATELPYAEWLHAGLIHLGDLPEREHVVHTAKSVA
                     RRQQTFGYTQEELRILLTPMARTGGEALGSMGTDTPIAVLSQKPRLLFDYFTQLFAQV
                     TNPPLDAIREELVTSLSTSVGPEGNVLTASPAHCRQLVLDFPVIDNDQLAKIIRINAD
                     GDLPGYATHVVKGLYDHMRDGEGMAARIDEICAEVSEAIARGARFIVLSDRDSGRDYA
                     PIPSLLLTAAVHHHLIREKTRTQVGLVVEAGDVREVHHVALLIGYGAAAVNPYLAMES
                     VEDLVRSGTITGVTGEQAVKNLIKALGKGVLKVMSKMGISTVASYRGAQVFEAIGLSQ
                     ELVDTYFTGTVTQLGGIGLDVIAAECAARHAAAYPPEGVRLPHRTLEVGGEYQWRREG
                     EPHLFDPE"
BASE COUNT          253 a          561 c          631 g          278 t
ORIGIN      
        1 ctcggggtcg aacagatgcg gttcgccttc gcggcgccat tggtattcgc cgccgacctc
       61 cagcgtgcgg tggggcagcc gcaccccctc cggcgggtat gccgcagcgt gcctcgccgc
      121 gcactccgcg gcgatcacgt cgagcccgat acccccgagc tgggtgacgg taccggtgaa
      181 gtaggtgtcg acgagttcct gagagaggcc gatggcctcg aagacctgag cgccgcgata
      241 ggacgccacg gtggagatgc ccatcttgga catcaccttc aggacgccct tgccgagcgc
      301 cttgatgagg ttcttgaccg cctgctcccc cgtgacgccg gtgatggtcc cggagcgcac
      361 gaggtcctcg accgactcca tggccagata ggggttgacc gccgccgcgc cgtagccgat
      421 gagcagggcg acgtggtgga cctcgcggac gtcgccggcc tcgacgacga gaccgacctg
      481 ggtgcgggtc ttctcccgga tgaggtggtg gtgaaccgcg gccgtcagca gcagcgacgg
      541 gatcggcgcg tagtcccggc cggagtcgcg gtcggacagg acgatgaacc gcgccccccg
      601 ggcgatggcc tcggagacct cggcgcagat ctcgtcgata cgcgccgcca ttccctcgcc
      661 gtcgcgcatg tggtcgtaga gacccttgac gacatgagtg gcgtagcccg gcaggtcgcc
      721 gtcggcgttg atgcggatga tcttcgcgag ctggtcgttg tcgatgaccg ggaagtcgag
      781 gaccagctgg cggcagtgcg ccggggaggc ggtcaggaca ttgccctcgg ggccgaccga
      841 ggtgctcagg gaggtgacga gttcctcgcg gatggcgtcc agcggcgggt tggtgacctg
      901 cgcgaacagc tgggtgaagt agtcgaacag tagccgcggc ttctggctga gcaccgcgat
      961 cggggtgtcc gtgcccatcg acccgagagc ctcaccgccg gtgcgggcca tcggggtgag
     1021 caggatccgc agctcctcct gggtgtagcc gaaggtctgc tgccgacgcg cgaccgactt
     1081 ggcggtgtgg acgacgtgct cccgctcggg caggtcgccc aggtggatca ggccggcgtg
     1141 cagccactcg gcatacggca gctcggtggc cagcttgctc ttgatctcct cgtcctcgat
     1201 gatccggccc tgggcggtgt cgacgaggaa catccggccc ggctgcagcc ggcccttgcg
     1261 gatgaccttc tcgggcgcga tgtcgagcac tccggactcg gacgccagca cgaccagccc
     1321 gtcgtcggtg acccagaacc ggcccgggcg caggccgttg cggtccagga ccgccccgat
     1381 ctgggtgccg tcggtgaagg tgacgcaggc cgggccgtcc cacggctcca tcgtcatcga
     1441 gtggaactcg tagaaggcgc gccggtcggc gtccatctcc tcgtggttct cccaggcctc
     1501 cgggatcatc atcagcacgg cgtgcgggag ggagcggccg cccaggtgga gcagttcgag
     1561 cacctcgtcg aaggaggcgg agtcggagcc gccgggcggg cagatcggcg acaagcgcga
     1621 caggtcgccg gggatcaggt cgctgaccag ctgggactcg cgggccgcca tccagttgcg
     1681 gttgcccttg acggtgttga tctcgccgtt gtgggcgatc agc
//
